LOCUS       DATMJB010000001         3664 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_59201, whole genome shotgun sequence.
ACCESSION   DATMJB010000001 DATMJB010000000
VERSION     DATMJB010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59201"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VNU48_00005"
     CDS             complement(<1..943)
                     /locus_tag="VNU48_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWI09690.1"
                     /translation="MQVKLNTIVAAAVVMLGANAYAQDMVVKIGHVGPTSGGIAHLGK
                     DNENGAKMAIDELNAKGVVIGGKKIKLELVPEDDAGDPKQGTAVAQKLVDSKVAGVIG
                     HLNSGTSIPASKIYSDAGIPQISPSATNPKFTRQGFKTTFRVVADDVHLGSTLGKYAV
                     KELKGKAVAVIDDRTAYGQGVAEEFTKAVKASGVPIVDTQFTTDKATDFTAILTQIKA
                     KKPDVIFFGGMDAVAGPMIRQMKQLGITSKFMGGDGICSAELPKLAGGAMADDQVVCA
                     EAGGVDATQQAALDAWKAKYKAKFGIDVQVYAPYVYDA"
     gene            complement(1114..1491)
                     /locus_tag="VNU48_00010"
     CDS             complement(1114..1491)
                     /locus_tag="VNU48_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09691.1"
                     /translation="MSAGPSVPPDRVPTLTEVVAWPEPAPPTAEPGGDAPARALAEAG
                     SGAAPTASPSFNEEQLTQRVLIDLQRQMDLMLDYRLREVLTPLLARAADGIVREARSE
                     LASTLRDVVERAVAQELARHRTR"
     gene            complement(1488..1919)
                     /locus_tag="VNU48_00015"
     CDS             complement(1488..1919)
                     /locus_tag="VNU48_00015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HWI09692.1"
                     /translation="MTEISFHFNVADRTDYTCRLVRKASRLGAAVVLAGPAEALTHFD
                     RALWTFDDLEFLPHVLLRPGQAVAPRQRATKVWLTQDAAQSGQHEVLINLGAQAPAGF
                     ESFEKVIEIVSVDEADRAAARVRWKHYKARGYAIREHGGGA"
     gene            complement(2047..3516)
                     /locus_tag="VNU48_00020"
     CDS             complement(2047..3516)
                     /locus_tag="VNU48_00020"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWI09693.1"
                     /translation="MDFHHQISSPDKLAALSCDALVLVVTGDAVEPGLATPLAAALSD
                     ALAHGDLELKAGKSLYLHRPSGVRAARVVFAAAGASTTKAFKAAVASGINLVKASGAK
                     HVAVGLVGAGALTDAHAEGVVIAAREATYLYRHTKPSAPAASKIDKVTVLCSRADAKA
                     VQQGLARGHAIAAGVNLAREWANRPANHCTPSDLAEQAKKLGKEFALKVEVLDRKACE
                     KLGMGSFLAVAQGSTEPLRFIVARYDGAAKSKVPQAPVVLVGKGITFDTGGISLKPGP
                     EMDEMKFDMGGAASVLGTLRAVAELKPKLNLVCIIPACENMPGGNAVKPGDVVKSMSG
                     QTIEILNTDAEGRLILCDALTYAERFKPAVVVDIATLTGACVIALGHHHSGLFTADDK
                     VAEQMLAASSKGLDPCWRMPLDEEYDAGLKSNFADMANIGGRPGGAITAAMFLRRFTA
                     KYPWAHLDIAGTAWKSGAAKGATGRPVALLTHFVLAQVA"
     gene            3544..>3664
                     /locus_tag="VNU48_00025"
     CDS             3544..>3664
                     /locus_tag="VNU48_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HWI09694.1"
                     /translation="MLFDSTLRRDLARNFGATLVVILTIVLTMMLIRTLGQAAG"
BASE COUNT          533 a         1277 c         1226 g          628 t
ORIGIN      
        1 gcgcgtcata gacgtaaggc gcgtagacct gcacgtcgat gccgaacttg gccttgtact
       61 tggccttcca cgcgtccagg gcagcctgct gggtcgcatc gacaccaccc gcttcggcgc
      121 agaccacctg gtcatcggcc atcgcgccgc cggccagctt gggcagttcg gccgagcaga
      181 tgccgtcgcc gcccatgaac ttggaggtga tgccgagttg cttcatctga cggatcatcg
      241 ggccggccac ggcgtccatg ccgccgaaga agatgacgtc gggcttcttg gccttgatct
      301 gggtcaggat ggcggtgaag tcggtcgcct tgtcggtcgt gaattgcgta tcgacgatgg
      361 gcacacccga ggccttgacg gccttggtga actcttcggc gacgccctgg ccgtaggccg
      421 tgcggtcatc gatcacggcg acggccttgc ccttcagctc cttgacggcg tacttgccga
      481 gcgtgctgcc cagatgcacg tcgtcggcca ccacgcggaa agtggtcttg aaaccttgac
      541 gggtgaactt ggggttggtc gccgacggcg agatctgcgg gatgccggca tcgctgtaga
      601 tcttggacgc ggggatggag gtgcccgagt tcaggtggcc gatcacgccg gcgaccttgg
      661 agtcgaccag cttctgcgcc acggccgtgc cttgcttcgg gtcgcccgcg tcgtcttcgg
      721 ggacgagctc gagcttgatc ttcttgccgc cgatgaccac gcccttggcg ttgagctcgt
      781 cgatggccat cttggcgccg ttctcgttgt ccttgccgag gtgcgcgatg ccgccgctgg
      841 tggggccgac gtgaccgatc ttgacgacca tgtcctgcgc gtacgcgttg gcaccgagca
      901 tcaccacagc cgcagcgacg atcgtgttca gtttgacttg catgcaaaac ctcctgatgg
      961 aatgttcaag gaaatacggt tgatgtctca acgcgcgaca caatacccca aaaaaacgca
     1021 ccctccacag tgaatgcacg ggtcgttcgc cgcaggcccg gctcgcaaca gcaagccggc
     1081 tcgcgcgctc ggggtctaac cacaacgcgc ggatcaacgc gtgcgatgac gcgccagttc
     1141 ctgcgccacc gcgcgctcga ccacgtcacg caaggtcgat gccagttcgc tgcgcgcttc
     1201 gcgcacgatg ccatcggccg cgcgcgcgag cagcggcgtc aacacttcgc gcaggcggta
     1261 gtcgagcatc agatccatct ggcgctgcag gtcgatcagc acgcgctgag tcagctgctc
     1321 ttcgttgaat gagggcgagg cggtgggcgc agcaccgctg cccgcctcgg ccaaagcgcg
     1381 cgcgggcgcg tcgccgcctg gctcggccgt gggcggcgcc ggttcgggcc aggccacgac
     1441 ctcggtcagc gtcggcacgc ggtccggcgg caccgacggc cccgcgctca cgcaccaccc
     1501 ccatgctcgc ggatcgcgta gccgcgggcc ttgtagtgct tccagcgcac acgcgccgcg
     1561 gcgcggtcgg cctcgtcgac cgagacgatc tcgatgacct tctcgaagct ctcgaacccg
     1621 gccggcgcct gcgccccgag gttgatcagc acctcgtgct ggccgctctg ggccgcgtcc
     1681 tgcgtcagcc agaccttggt cgcgcgctgc ctgggtgcga ccgcctggcc cgggcgcagc
     1741 agcacgtgcg gcaggaactc gagatcgtcg aaggtccaca acgcgcggtc gaaatgcgtc
     1801 agcgcctcgg ccgggccggc cagcacgaca gcggcgccga gccggctggc cttgcgcacc
     1861 agccggcagg tgtagtcggt gcggtccgcc acgttgaagt ggaagctgat ctctgtcaat
     1921 ggagcccctc gcccgccgag tacggcggtc gatccccagg ggccactagg ggccccttcg
     1981 tggcccttgg gatgcgggcc ggcttgggag cggcccggcg ctcggcccgc tgaggcctca
     2041 cttggatcat gcgacctgcg ccagcacgaa gtgcgtcagc agcgccaccg gccggccggt
     2101 ggcgcccttg gcagcgcccg acttccacgc cgtgccggcg atgtccaggt gcgcccacgg
     2161 gtacttggcc gtgaagcggc gcaggaacat cgccgccgtg atcgcgccgc ccgggcgccc
     2221 gccgatgttg gccatgtcgg cgaagttgct cttcagcccg gcgtcgtatt cctcgtccag
     2281 cggcatgcgc cagcacgggt ccaggccctt gctgctggcc gcgagcatct gctcggcgac
     2341 cttgtcgtcg gcggtgaaca ggcccgaatg gtggtgcccc agcgcgatca cgcaggcacc
     2401 ggtcagcgtc gcgatatcga ccacgaccgc gggcttgaag cgctcggcgt aggtcagcgc
     2461 atcgcacagg atcaggcgcc cttcggcgtc ggtgttcagg atctcgatgg tctggcccga
     2521 catgctcttg accacgtcgc ccggcttcac ggcgttgccg cccggcatgt tctcgcatgc
     2581 gggaatgatg cagacgaggt tgagcttggg cttcagctcg gcgaccgcgc gcagcgtgcc
     2641 gagcacgctc gcggcaccgc ccatgtcgaa cttcatctcg tccatctcgg gcccgggctt
     2701 cagcgagatg ccaccggtgt cgaaggtgat gcccttgccg accagcacca ccggcgcctg
     2761 cggcaccttc gatttggccg cgccgtcgta gcgcgcgacg atgaagcgca gcggctcggt
     2821 cgagccctgc gcgaccgcga ggaacgagcc catgccgagc ttctcgcagg ccttgcggtc
     2881 cagcacctcg accttcagtg cgaattcctt gcccagcttc ttcgcctgct cggcgaggtc
     2941 actcggcgtg cagtgattgg ccggacggtt cgcccattcg cgcgccaggt tcacgcccgc
     3001 ggcgatcgcg tggccgcgcg ccaggccctg ctgcacggcc ttcgcgtcgg cccggctgca
     3061 cagcaccgtg accttgtcga tcttcgatgc ggcgggcgcg ctcggcttgg tgtggcggta
     3121 caggtaggtg gcctcgcgcg ccgcgatgac cacgccttcc gcgtgcgcat cggtcagcgc
     3181 gccggcgccg accaggccga ccgcgacatg cttcgcgccg ctggccttga ccaggttgat
     3241 gccgctcgcc acggcggcct tgaacgcctt ggtcgtcgac gcaccagcgg ccgcgaacac
     3301 gacgcgcgcg gcgcgcacgc cggacggccg gtgcaggtac aggctcttgc cggccttgag
     3361 ctcgaggtcg ccgtgcgcca gcgcgtcgct caaggccgcg gccagcggcg tggccaggcc
     3421 cggctcgacc gcgtcgccgg tgacgaccag cacgagcgcg tcgcagctca gcgccgccag
     3481 cttgtccggc gaagaaatct gatgatgaaa gtccataatc ggcagtccat tagacagccg
     3541 atcatgttat tcgattccac tttgcgccga gacctggcgc gcaacttcgg tgccacgctg
     3601 gtcgtgatcc tcaccatcgt gctgacgatg atgctgatcc gcaccctcgg ccaggccgcg
     3661 ggcg
//
LOCUS       DATMJB010000002         5147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_650973, whole genome shotgun sequence.
ACCESSION   DATMJB010000002 DATMJB010000000
VERSION     DATMJB010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650973"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VNU48_00030"
     CDS             <1..848
                     /locus_tag="VNU48_00030"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HWI09695.1"
                     /translation="PARSAVGAGMTRILAVCADDFGLAPGLSAGIARLAHAQRLTAVS
                     CITNSPHWGAAAALLANLPDSVDVGLHLNFSEGRPLSARLARRWPRLPALPRLIAQAH
                     LGLLPRSEVRNEVHAQLAAFNEAFGRPPRFIDGHQHVHHLPGLRQIVLDMVEHVQPLP
                     ALRSTARVLGPGFGLKRWLIERTGGRALERELAQRVMARNPALLGAYDFVDPNYQALM
                     RRWLAVLPAEGGLLFCHPGEHRPGDPPDAIGAARERELAYLGSVAFDTDLAAADVRLG
                     RVWRT"
     gene            complement(851..1213)
                     /locus_tag="VNU48_00035"
     CDS             complement(851..1213)
                     /locus_tag="VNU48_00035"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWI09696.1"
                     /translation="MRHFFRYSTVGAFATATHYLVLVAAVEGAGWPAYLGSGVGAVVG
                     AQVAYAGNRWFTFGHRGAMRASWPRFQATALVGALLGMAIVALGVRLGVHYLVAQVAA
                     TLASLLLTFAINRAWTFR"
     gene            complement(1220..2737)
                     /locus_tag="VNU48_00040"
     CDS             complement(1220..2737)
                     /locus_tag="VNU48_00040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWI09697.1"
                     /translation="MPALSNDRRDARLSSAPVLLALLALWLLATLGARPLLLPDEGRY
                     AEVARAMWHGDLWVPTLNGLPFFHKPPLFYWLDIAALQLVGSNVFAGRFGSALGAWLM
                     GASLFLALRRWHGARTARVALGVLATTPFFFVGAQYANHDMLVGGLITAAVLALARAL
                     DAPPRVELRWLVAGWVLCALAMLAKGLIGIVLPALVFGPWLLAQGRWRQMVKLLHPLG
                     LLAFALVGLPWFVAVQWRYPDFFDYFVIEQHFRRFAQSNFNNAHGAWFFIAAMPLLTL
                     PWSAWLPAAVRCVSGPRAAAQAGPPGTIGLYAWWIVAIIGFFSIPSSKLVGYALPALA
                     PWCALIALAIATGTTRIWRRVMVASAIVCVAIVAVVAWKAPNSNRALALTLASNAAPA
                     DRVVMVDEYYYDVPFYARLARPVVIASDWADPSIPTRDNWRKEVFDAGRFDPALAHEL
                     LRPLTELPHMACGASAVWFIVAAGNAARVSGLPSATRVFADRRTELWRAPGRTCL"
     gene            2812..3471
                     /locus_tag="VNU48_00045"
     CDS             2812..3471
                     /locus_tag="VNU48_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWI09698.1"
                     /translation="MDAEAGLSMPLFPLRTVLFPGGLLPLQIFELRYLDMIARRHRAG
                     APFGVVCLTEGSEVRRPAAEPGGDGFAHEAFHPIGTLARIDELQRPRPGLLLVRCTGT
                     QRFRVLRSAQQKFGLWVAEVEPIADDALVPVPDDLGFAREALVALLRELERDADAAEL
                     PMHPPYRWDDCGWLANRWCELLPIDATSKQRFMAIDSPLLRLELVADTLRQMGLARPG
                     D"
     gene            complement(3481..4017)
                     /locus_tag="VNU48_00050"
     CDS             complement(3481..4017)
                     /locus_tag="VNU48_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HWI09699.1"
                     /translation="MDSIDRRRLLLAGTARAQPAWPTKPVRVVVPFAPAGTTDLLARA
                     LSPELSKAFGQTFIIDNKPGAGGNLGADAIAKSAPDGYELLMGTVGRHAINGAVAGFD
                     ASSWFGLFAPAGTPMEIVNRIQQETAKAFHAPAFKERLLSQGAIPGGQPPAEFAKFIA
                     AETKKWAQVVKASGAKVD"
     gene            complement(4227..4385)
                     /locus_tag="VNU48_00055"
     CDS             complement(4227..4385)
                     /locus_tag="VNU48_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09700.1"
                     /translation="MNAPRPLNARTVLPPVRPPVLLAVPRQPFQVLQTGRAPAQLSLF
                     LRPARYAR"
     gene            complement(4382..4591)
                     /locus_tag="VNU48_00060"
     CDS             complement(4382..4591)
                     /locus_tag="VNU48_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09701.1"
                     /translation="MSLTRSAKTFAPRRPRPATPHCRGGASSVLRLTHAGAPRLPNGA
                     DLADWMSRAAPELMPVTGPLAAGAR"
     gene            complement(4774..>5147)
                     /locus_tag="VNU48_00065"
     CDS             complement(4774..>5147)
                     /locus_tag="VNU48_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI09702.1"
                     /translation="DEAAMDKYPHEFSGGQRQRIAIARCLTLDPEVLVLDEAVSALDV
                     SVQAQVLNLLKDLQDELGLAYIFISHDLAVVRFMADEVLVMKDGEVVEQASVAQILDA
                     PQQDYTRRLLGAIPRGYRAAA"
BASE COUNT          766 a         1872 c         1862 g          647 t
ORIGIN      
        1 ggccggcgcg cagcgccgtg ggggccggca tgacgcggat actggccgtc tgcgccgacg
       61 acttcgggct cgcgcccggc ctctcggccg gcatcgcgcg cctcgcgcat gcgcagcggc
      121 tcacggccgt ctcgtgcatc accaacagcc cgcactgggg cgcggccgcg gcgctgctgg
      181 cgaatctgcc cgacagcgtc gacgtcggcc tgcacctgaa cttcagcgaa ggccgcccgc
      241 tcagcgcccg gctggcgcgg cgctggccgc ggctgcctgc gctgccgcgg ctgatcgcgc
      301 aggcgcacct cgggctgctg ccgcgttccg aggtgcgcaa cgaggtccat gcgcagctcg
      361 ccgcgttcaa cgaggcgttc ggccggccgc cgcgcttcat cgatggtcat cagcacgtgc
      421 accacctgcc ggggctgcgc cagatcgtgc tcgacatggt cgagcacgtg cagccgctgc
      481 cggcgctgcg cagcaccgcg cgcgtgctcg ggccgggctt cggcctgaag cgctggctga
      541 tcgagcgcac cggcggccgg gcgctcgaac gcgaactcgc gcaacgcgtg atggcgcgca
      601 acccggcgct gctcggcgcc tacgacttcg tcgacccgaa ctaccaggcg ctgatgcggc
      661 gctggctcgc cgtgctgccg gccgagggcg ggctgctgtt ctgccacccc ggcgagcacc
      721 ggccgggcga cccgccggac gcgatcggcg cggcgcgcga gcgcgaactc gcctacctcg
      781 gcagcgtcgc attcgacacc gacctggccg ccgcggacgt gcggctcggc cgcgtctgga
      841 gaacctagcg tcagcggaag gtccaggccc ggttgatcgc gaacgtcagc agcaggctcg
      901 cgagcgtcgc ggcgacctgc gcgaccaggt agtgcacgcc cagccgcacc cccagcgcga
      961 cgatcgccat gccgagcagc gcgccgacga gcgcggtggc ctggaagcgc ggccacgacg
     1021 cccgcatcgc gccgcggtgg ccgaaggtga accagcggtt gccggcgtac gcgacctgcg
     1081 cgccgacgac cgcgcccacg cccgagccga ggtaggccgg ccagcccgcc ccttcgaccg
     1141 cggccaccag caccaggtag tgcgtcgcgg tcgcgaacgc gccgacggtg ctgtagcgga
     1201 agaaatggcg catcgccggt cagaggcagg tgcggccggg cgcgcgccag agctcggtgc
     1261 gccggtcggc gaacacccgt gtcgcgctcg gcaggcccga gacccgcgcc gcgttgccgg
     1321 ccgccacgat gaaccagacc gcgctcgcgc cgcaggccat gtgcggcagc tcggtcagcg
     1381 gccgcagcag ttcgtgcgcg agcgcggggt cgaagcggcc ggcatcgaag acttccttgc
     1441 gccagttgtc gcgcgtcggg atcgacgggt cggcccagtc gctcgcgatc acgaccggcc
     1501 gcgccagccg cgcgtagaac ggcacgtcgt agtagtactc gtcgaccatc acgacgcggt
     1561 cggccggtgc cgcgttgctg gccagtgtca gcgccagcgc gcggttcgag ttcggcgcct
     1621 tccaggcgac gaccgcgacg atcgcgacac agacgatcgc cgacgccacc atcacgcggc
     1681 gccagatgcg cgtcgtgccg gtcgcgatcg ccagcgcgat cagcgcgcac catggcgcga
     1741 gcgccggcag cgcgtagccg acgagcttgg agctcgggat cgagaagaag ccgatgatcg
     1801 cgacgatcca ccacgcgtac aggccgatcg ttccgggcgg gccggcctgg gcggcggcgc
     1861 gcggcccgct cacgcagcgc acggcggccg gcagccaggc gctccacggc aaggtcagca
     1921 gcggcatcgc ggcgatgaag aaccaggcac cgtgcgcgtt gttgaagttc gactgcgcga
     1981 agcgccggaa atgctgctcg atcacgaaat agtcgaagaa gtccgggtag cgccactgca
     2041 ccgcgacgaa ccacggcagc ccgacgagcg cgaacgcgag cagcccgagc ggatgcagca
     2101 gcttcaccat ctgccgccag cgcccctgcg cgagcagcca cgggccgaac accagcgccg
     2161 gcagcacgat gccgatcagc cccttcgcga gcatcgccag cgcgcacagc acccagcccg
     2221 cgacgagcca gcgcaactcc acacgcggcg gcgcatccag tgcacgcgcc agcgccagca
     2281 ccgccgcggt gatcaggccg ccgaccagca tgtcgtggtt cgcgtactgt gcaccgacga
     2341 agaagaacgg cgtcgttgcg agcacgccca gcgcgacgcg tgcggtgcgc gcgccgtgcc
     2401 agcgccgcag cgccaggaac agcgacgcgc ccatcagcca cgcccccagc gccgagccga
     2461 agcggcccgc gaacacgttg ctgccgacga gctgcagcgc ggcgatgtcg agccagtaga
     2521 acagcggcgg cttgtggaag aacggcaggc cgttcagcgt cggcacccac aggtcgccgt
     2581 gccacatcgc gcgcgcgacc tcggcgtagc ggccctcgtc gggcagcagc agcgggcgcg
     2641 cgccgagcgt cgcgagcagc cacaacgcga gcagcgcgag cagcaccggc gccgagctca
     2701 ggcgggcatc gcggcggtcg ttcgagaggg cgggcatcgg cgggggcgag ggtcggtgtg
     2761 cattagacca tcggccagcg cgccgccgcg cgctgcgctt agcatcgcgc gatggatgcc
     2821 gaggccggcc tgtcgatgcc gctgttcccg ttgcgcaccg tgctgttccc gggcgggctg
     2881 ctgccgttgc agatcttcga gctgcgctac ctcgacatga tcgcgcgccg gcaccgcgcc
     2941 ggcgcgccgt tcggtgtggt gtgcctgacc gaaggcagcg aggtgcggcg ccccgccgcg
     3001 gagccgggcg gcgacggctt cgcgcacgaa gcctttcacc cgatcggcac gctcgcgcgc
     3061 atcgacgagc tgcagcgccc gcggcccggg ctgctgctgg tgcgctgcac cggcacgcag
     3121 cgctttcgcg tgctgcgcag cgcgcagcag aagttcgggc tgtgggtcgc cgaggtcgaa
     3181 ccgatcgccg acgacgcgct cgtgccggtg cccgacgacc tcgggttcgc gcgcgaggcg
     3241 ctggtcgcgc tgctgcgcga gctcgagcgc gacgccgacg ccgccgagct gccgatgcac
     3301 ccgccctacc gctgggacga ttgcggctgg ctggcgaacc gctggtgcga attgctgccg
     3361 atcgacgcga cctcgaagca gcgcttcatg gcgatcgata gcccgctgct gcgcctcgaa
     3421 ctcgtcgccg acaccttgcg tcagatgggg ctggcgcgcc ccggcgattg agagccgagg
     3481 tcagtcgacc ttcgcgcccg aggccttcac gacctgggcc cacttcttcg tttcggcggc
     3541 gatgaacttc gcgaactcgg ccggcggctg gccgcccggg atcgcgccct gcgacagcag
     3601 ccgctccttg aaggccggcg catggaaggc cttcgcggtc tcctgctgga tgcggttgac
     3661 gatctccatc ggcgtgcccg ccggcgcgaa caggccgaac cacgagctcg cgtcgaagcc
     3721 cgccaccgcg ccgttgatgg cgtgcctgcc gaccgtgccc atcagcagct cgtagccgtc
     3781 gggcgcggac ttggcgatcg cgtcggcgcc caggttgcca ccggcgccgg gcttgttgtc
     3841 gatgatgaag gtctggccga aggccttgct cagctcgggc gacagcgcac gcgccagaag
     3901 gtcggtggtg ccggccggcg cgaacggcac gaccacgcgc accggcttcg tcggccatgc
     3961 cggttgggca cgcgccgtgc cggccagcag caggcggcga cgatcgatcg aatccatggg
     4021 gagcccttcg ccagacaagc gctgcgcgag agtgtctcgt gcggggcgca acgaggcaga
     4081 gggcttgccc gcagcggcgc cggacggcgc cgcgaaaaaa gaagggaggc gggacagtgc
     4141 gttgccgagg ttgctctcga atcgcctcgg cctgcccgcc tccctgagga cgtcaggacc
     4201 gtccgtgaaa aaggaaggaa gcaggctcag cgcgcgtagc gcgccggccg caggaacagc
     4261 gacagctgcg ccggcgcgcg gccggtctgc agcacttgaa acggctgacg cggaacggcc
     4321 agcagcacgg gcggccgcac cggcggcaac acggtgcgcg cgttgagcgg acgcggcgcg
     4381 ttcatcgggc ccccgcggcg agcgggccgg tgacgggcat cagctcgggt gcggcgcggc
     4441 tcatccagtc cgccaggtcc gcgccgttcg gcaggcgcgg ggcgccggcg tgcgtcaggc
     4501 gtagcaccga cgaggcgccg ccgcggcaat gcggcgtggc cggccgcggg cggcgcggcg
     4561 cgaaggtctt ggcggagcgg gtcagcgaca tggcaacgac gtcctcggga tgctgtgcac
     4621 ggtcatgcgc aacgtccgtg ccatgcccgc tcggctcatc gagtgacctg cggtggacaa
     4681 atctcgacac ggaatcgaac cagggcctac gaaatctgac gcagtcgtaa gggcggcgtt
     4741 acagcctcga ccggattggt cggcgccgcg cgctcacgcg gccgcccggt accctcgcgg
     4801 aatcgccccc agcagccgcc gcgtgtagtc ctgctgcggc gcgtccagga tctgcgcgac
     4861 gctggcctgc tcgacgacct cgccgtcctt catcaccagc acctcgtcgg ccatgaagcg
     4921 caccacggcc aggtcgtggc tgatgaagat gtaggcgagg ccgagctcgt cctgcaggtc
     4981 cttcagcagg ttcagcacct gggcctgcac cgacacgtcg agcgcgctga ccgcctcgtc
     5041 gagcaccagc acctccgggt ccagcgtcag gcagcgggcg atcgcgatgc gctggcgctg
     5101 gccgccggaa aactcgtgcg ggtatttgtc catcgccgcc tcgtcca
//
LOCUS       DATMJB010000003         3806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_650181, whole genome shotgun sequence.
ACCESSION   DATMJB010000003 DATMJB010000000
VERSION     DATMJB010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650181"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VNU48_00070"
     CDS             <1..191
                     /locus_tag="VNU48_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09703.1"
                     /translation="FDTAQADLQAAQSALDRYFDSSSKRTALAGELIRQVVGQARRVV
                     VPRPDDTLAALTAASGGR"
     gene            202..1449
                     /locus_tag="VNU48_00075"
     CDS             202..1449
                     /locus_tag="VNU48_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HWI09704.1"
                     /translation="MRNVIWFVLLFGVAVLAASALGTNDGLVTFYWRGWRVDVSLNLF
                     LLGLLGSCFVIVTAIQALSALVGLPRRAHEWRVARRDRSAQAALRDALAQYFGGRYSR
                     AQKSAQRALVIQADTPELAQDNEFTVLSHLLAAGSAHRLQDRPARDEQLRHALDLSRR
                     SAAARSAEEGARLLAAEWALDDRDATRALALLGELPQGVARRTHALRLRLQATRLGRQ
                     PQEALKTARLLAKHQGFSKIAAQGLLRSLAFESLDTAHDIDQLKRVWGQFEPVDRRDA
                     FVAARAADRAAALGAFDDARGWLRPFWEQSTELGADERAAVALGLVSAMRGIGLEWLP
                     RLEAAASAYSREGAVALAVGCALAERQLWGKARRLLEQAAADGALANAPRRKAWLMLA
                     ELARQEGDAARAAVCFEAAARLG"
     gene            1483..1559
                     /locus_tag="VNU48_00080"
     tRNA            1483..1559
                     /locus_tag="VNU48_00080"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1517..1519,aa:Arg,seq:acg)
     gene            1898..2539
                     /locus_tag="VNU48_00085"
     CDS             1898..2539
                     /locus_tag="VNU48_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HWI09705.1"
                     /translation="MNRGSSARVEESESGERWGAAVMEPIEELRMDKSMIKGMAVGGV
                     AMVVLGSGAVTGYKAITKPQFAEVVAVKDVVETVVTPQEKCEDVQVRHQAPVKDQHRI
                     AGSVVGGLAGGLLGSVVGGGKGKTIATVAGAAAGGYAGNQVQKNMQEKDVVTTTEHRC
                     KTVNERSQKVVGYNVTYRLDGKDGTARMSFKPGPTLPVKDGKVVTTPIADNKS"
     gene            2835..>3806
                     /locus_tag="VNU48_00090"
     CDS             2835..>3806
                     /locus_tag="VNU48_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HWI09706.1"
                     /translation="MPVDARERLRVVVGAAIGVLLTGLLCRALGAPGAVLPWLAAPIG
                     ASAVLVFGVPGSPMAQPWAVIAGNTVSALAGIVCVLEFGSGPWVAALAVGLAIAAMFA
                     LRCLHPPGGACALLTVLGGVADPHFALSPVLLNSVMLVMAGVAYNGATGRRYPHGQHA
                     APSPDTAVQRFDEVDLDAVIARYNQVLDIGRDDLQNLLGQAELEAYRRKMGATRCADI
                     MSRQLVTAEFGTSLQDAWARLRAKGIKALPVVDRAARVVGIVTLADFLRGAELDLHAG
                     WAGRLRAMIRATTTSHSDKAEVVGQIMTRRVRVASAGRPISELVPMFA"
BASE COUNT          576 a         1294 c         1360 g          576 t
ORIGIN      
        1 agttcgacac cgcacaggcg gatctgcagg ccgcgcagtc ggcactcgac cgctacttcg
       61 acagcagctc gaagcgcacc gcattggctg gcgagctgat tcgccaggtc gtcgggcagg
      121 cgcggcgggt ggtcgtgccg cgacctgacg acaccctggc cgcgctcacc gccgcgtccg
      181 gcggccgctg agcgcgcggt catgcgcaac gtcatctggt tcgtgctgtt gttcggcgtc
      241 gccgtgctgg ccgcgtccgc gctcggcacc aacgacggtc tcgtgacgtt ctactggcgc
      301 ggctggcggg tcgacgtctc gttgaatctg tttctgctcg ggctgctcgg cagctgcttc
      361 gtgatcgtca ccgcgatcca ggcgctgagc gcgctcgtcg ggctgccgcg gcgtgcgcac
      421 gagtggcgcg tcgcgcgccg cgatcgcagc gcacaggcgg cgctgcgcga tgcgctggcg
      481 cagtacttcg gtggccgcta cagccgcgcg cagaaatcgg cgcagcgcgc cctcgtgatc
      541 caggccgaca cgcccgaact cgcgcaggac aacgagttca ccgtgctgag ccacctgctc
      601 gccgccggca gcgcgcaccg cctgcaggac cgcccggccc gcgacgagca gctgcgccac
      661 gcgctcgatc tgtcgcggcg cagcgccgcg gcgcgctcgg ccgaagaggg cgcgcggctg
      721 ctcgccgccg agtgggcgct cgacgaccgc gacgcgacgc gcgcgctcgc gctgctgggc
      781 gagctgccgc agggcgtcgc gcgccgcact catgcgctgc gattgcgcct gcaggccacg
      841 cgcctgggtc gtcagccgca ggaggccttg aagaccgcac gcctgctcgc caagcaccag
      901 ggcttctcga agatcgcggc gcaaggcctg ctgcgctcgc tcgcgttcga gtcgctcgac
      961 accgcccacg acatcgacca gctcaagcgc gtgtgggggc agttcgagcc ggtcgatcgg
     1021 cgtgatgcct tcgttgctgc gcgcgccgcg gaccgagccg ccgcactcgg cgcgttcgac
     1081 gatgcacgcg gctggctgcg cccgttctgg gagcaatcca ccgaactcgg cgccgacgag
     1141 cgcgcggcgg tggcgctcgg gctcgtcagt gcgatgcgcg gcatcggcct cgaatggctg
     1201 ccgcggctcg aggcggcggc gagcgcgtac tcgcgcgagg gcgccgtcgc gctggcggtg
     1261 ggttgtgcac tggccgagcg ccagctctgg ggcaaggcgc ggcggctgct cgagcaggcc
     1321 gccgccgatg gcgcgctcgc gaacgcgccg cgccgcaagg cctggctgat gcttgcggaa
     1381 ctggcccgcc aggaaggcga cgcggcgcgc gcggcggtgt gcttcgaagc ggcggctcga
     1441 ctgggctgac gcggacgcgg cgatgctata ctccatggct gcgcggctgt agctcagctg
     1501 gatagagtac ttggctacga accaaggggt cgtgggttcg attcctgcca gccgcaccat
     1561 aaagcacgag gggtagcacg aaagtaagtg ctaccccttc gtcgttccag agggccgcgg
     1621 ctacctgtcg gccaccagca ataccgcgag ggtcttttgg cgcatgttgt tgtcgtatgc
     1681 gcgatctgca cgcatgccgc agcccagagt ccggtccaag cgtttggaac agccgcagca
     1741 gtccaacaaa aaatgggcca actgtgtgca agcatcgcgc atggctaacc aaccatcggc
     1801 caacggctga attgatcgac gctgcgagat tggtcagcct gaacccagat tcagccggcc
     1861 gatgcgcgca acctcttacg attgatcaca acgtgtcgtg aaccgtggca gcagcgcccg
     1921 cgttgaggag tcagagtcgg gcgagcgctg gggcgccgcc gtcatggaac caatcgagga
     1981 gttgcgcatg gacaaatcaa tgatcaaggg aatggccgtc ggtggcgtcg ccatggtggt
     2041 gctcggctcc ggcgccgtca cgggctacaa ggcgataacc aagccgcagt ttgccgaagt
     2101 cgtcgcggtg aaggatgtcg tcgagacggt ggtcacaccg caggagaaat gcgaagacgt
     2161 ccaggttcga catcaggcgc cggtgaagga tcaacatcgc atcgccggca gcgtggtcgg
     2221 cggactggcg ggcgggctgc tgggaagcgt cgtcggtggc ggcaagggca agacgatcgc
     2281 caccgtcgcc ggagccgccg cgggcggcta tgccggcaac caggtccaga agaacatgca
     2341 agagaaagat gtcgtcacga cgaccgagca ccgctgcaag accgtgaacg aaaggtcgca
     2401 gaaggtggtc ggctacaacg tgacctatcg cctggatggc aaggatggca cggcgcgcat
     2461 gtccttcaag ccggggccga cgctgccagt gaaggatggg aaagtggtca cgacgccgat
     2521 cgccgacaac aagtcgtagt tgcgcaccaa cgtcgaacga tcgtttgggc cgggcacgcg
     2581 gaagacgata cgagaaggcc tggccgcgct gagcgctcgt gttcgaggca cgggacacgc
     2641 gccgtttgtc caatctcgaa gaacttgacc tcggtcaagc gccgttgcgg ccgtgccgac
     2701 gcatgctcgc cccgcgccgc cgccgccgac gccgcgctgc catgtccacc accccgatca
     2761 cgacccccac aacccctgcc gcacccaggc gacgcccgct ctccgcctgg gcccgcgacc
     2821 tgctgcccgc acgcatgccg gtcgacgcgc gcgaacggct gcgcgtcgtc gtcggtgccg
     2881 caatcggcgt gctgctcacc gggctgctgt gtcgcgcgct gggcgcgccc ggcgcggtgc
     2941 tgccgtggct ggccgcgccg atcggcgcga gtgccgtgct cgtgttcggc gtgccgggca
     3001 gcccgatggc gcagccctgg gccgtcatcg ccggcaacac cgtgtcggcg ctggcaggca
     3061 tcgtgtgcgt gctcgagttc ggcagcgggc cgtgggtcgc cgcgctcgcg gtcggtctcg
     3121 cgatcgcggc gatgttcgcg ttgcgttgcc tgcacccgcc gggtggcgcg tgcgcgctgc
     3181 tgaccgtgct cggcggcgtc gccgacccgc atttcgccct ttcgccggtg ctgctgaact
     3241 cggtcatgct ggtgatggcc ggcgtggcgt acaacggcgc caccgggcgg cgctacccgc
     3301 acggccagca cgcggcgcca tcgcccgaca cggcggtgca gcgcttcgac gaggtcgatc
     3361 tggacgccgt gatcgcgcgc tacaaccagg tcctggacat cggccgcgac gacttgcaga
     3421 acttgcttgg gcaggccgag ctggaagcct atcggcgcaa gatgggcgcc acgcgctgtg
     3481 ccgacatcat gtcgcgccag ctcgtgacgg ccgaattcgg cacgtcgctg caggacgcct
     3541 gggcgcggct gcgagccaag ggcatcaagg cgctgccggt ggtcgaccgg gccgcgcgcg
     3601 tcgttggcat cgtgacgctg gccgactttc tcaggggcgc cgaactcgac ctgcatgccg
     3661 gctgggccgg gcgcttgcgc gcgatgatcc gcgcgaccac cacgtcgcat tcggacaagg
     3721 ccgaggtcgt cggccagatc atgacgcgcc gggtgcgcgt cgcgagcgcc ggccgcccga
     3781 tcagcgagct ggtgccgatg tttgcc
//
LOCUS       DATMJB010000004         3559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_355230, whole genome shotgun sequence.
ACCESSION   DATMJB010000004 DATMJB010000000
VERSION     DATMJB010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355230"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /gene="rplD"
                     /locus_tag="VNU48_00095"
     CDS             complement(<1..356)
                     /gene="rplD"
                     /locus_tag="VNU48_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWI09707.1"
                     /translation="MQLELLNEQGQATSKVDAPDTLFGRDYNEALVHQIVVAFQANAR
                     QGTRAQLDRTMVKHSTKKPWRQKGTGRARAGMTSSPLWRGGGRIFPNSPDENFTQKVN
                     KKMYRAGMAAIFSQLAR"
     gene            complement(358..1071)
                     /gene="rplC"
                     /locus_tag="VNU48_00100"
     CDS             complement(358..1071)
                     /gene="rplC"
                     /locus_tag="VNU48_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HWI09708.1"
                     /translation="MTTVNPGHRLGLLGRKVGMMRIFTDDGDAIPVTVLDVSNNRVAQ
                     IKTEQTDGYSAIQVAFGERKASRVSKPEAGHFAKAGVEAGEILQEFRVAAEVAAEYKP
                     GAHVVATGVFAVGQLVDVQGTSLGKGFTGTIKRHNFKSQRASHGNSRSHNVPGSISMA
                     QDPGRVFPGKKMSGQRGNVTKSVQNLDIVRIDEARQLLLVRGAVPGHKNGHVVVSPAI
                     KVKVARGGAAVAKPAKGAN"
     gene            complement(1272..1583)
                     /gene="rpsJ"
                     /locus_tag="VNU48_00105"
     CDS             complement(1272..1583)
                     /gene="rpsJ"
                     /locus_tag="VNU48_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HWI09709.1"
                     /translation="MQKQKIRIRLKAFDYKLIDQSALEIVDTAKRTGAIVKGPVPLPT
                     RMQRFDILRSPHVNKSSRDQFEIRTHQRLMDIVDPTDKTVDALMKLDLPAGVDVEIKL
                     Q"
     gene            complement(1685..2875)
                     /gene="tuf"
                     /locus_tag="VNU48_00110"
     CDS             complement(1685..2875)
                     /gene="tuf"
                     /locus_tag="VNU48_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HWI09710.1"
                     /translation="MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLSKLFGGEA
                     KAYDQIDAAPEEKARGITINTAHVEYETKNRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIIVYMNKCDMVDDAELLELVEMEVRELLDK
                     YEFPGDATPIIHGSAKLAMEGDKGELGEGSILKLADALDSYIPTPERAIDGAFLMPVE
                     DVFSISGRGTVVTGRVERGVIKVGEEIEIVGITPTQKTTCTGVEMFRKLLDQGQAGDN
                     VGILLRGTKREDVQRGQVLCKPGSVKPHTHFTAEIYVLSKEEGGRHTPFFNNYRPQFY
                     FRTTDVTGAVELPKDKEMVMPGDNVSITVKLINPIAMEEGLRFAIREGGRTVGAGVVA
                     KIIE"
     gene            complement(2996..>3559)
                     /gene="fusA"
                     /locus_tag="VNU48_00115"
     CDS             complement(2996..>3559)
                     /gene="fusA"
                     /locus_tag="VNU48_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923501.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWI09711.1"
                     /translation="GQVVLKLEPQAPGKGFEFVDATKGGVVPKEFIPAVKKGIEDSLP
                     AGVLAGYPVVDVKVTLTFGSYHEVDSNENAFKMAASLGFKDGCRKASPVILEPMMAVE
                     VETPEDYAGSVMGDLSSRRGMVQGMDDIPGGGKIIKAEVPLSEMFGYSTTLRSMSQGR
                     ATYTMEFKHYSEAPKNVADAIITARGK"
BASE COUNT          506 a         1120 c         1174 g          759 t
ORIGIN      
        1 cgggccaact gcgagaagat cgcagccatg ccggcgcggt acatcttctt gttgaccttc
       61 tgggtgaagt tttcgtccgg gctgttcggg aaaatccgac cgcctccgcg ccacagcggc
      121 gacgaggtca tgccggcgcg cgcgcggcca gtgcccttct ggcgccacgg cttcttcgtg
      181 ctgtgcttga ccatcgtgcg gtcgagctgg gcgcgggtgc cctggcgtgc gttggcctgg
      241 aaggccacga cgatctggtg caccagcgct tcgttgtagt cgcggccgaa cagcgtgtcc
      301 ggcgcgtcga ccttgctcgt cgcctggcct tgttcgttga ggagttcgag ctgcatgtca
      361 gttcgctccc ttggcaggct tcgcgacggc tgcgccaccg cgggcaacct tgaccttgat
      421 cgccgggctc acgaccacat ggccgttctt gtgcccgggc accgcgccgc gcaccagcag
      481 cagctggcgc gcttcgtcga tgcggacgat gtccaggttc tggacgctct tggtcacgtt
      541 gccgcgctgc ccagacatct tcttgcccgg aaacacgcgg cccggatcct gcgccatcga
      601 gatcgagccc ggcacgttgt gcgaacggct gttgccgtgt gacgcgcgct gcgacttgaa
      661 gttgtggcgc ttgatcgtgc cggtgaagcc cttgccgagc gacgtgcctt gcacatccac
      721 cagctggccc acggcgaaca cgcccgtggc aacgacgtgc gcgccgggct tgtattcggc
      781 cgccacttcg gcggccacgc ggaattcctg gaggatctcg ccggcttcca cacccgcctt
      841 ggcgaagtgg cccgcttcgg gcttggaaac acgggatgcc ttgcgttcgc cgaacgcgac
      901 ctggatggcg ctgtagccgt cggtctgttc ggtcttgatc tgggcaacgc ggttgttcga
      961 cacgtcgagc accgtcacgg gaatggcgtc gccatcgtcc gtgaagatgc gcatcatgcc
     1021 caccttgcgg cccagcaaac cgaggcggtg gcctggattg actgtcgtca tggttcttct
     1081 ccagctccct ggctttgcag ccgcggcagc ctttgttcac gagcccgaca tcgattggtc
     1141 gggttgattg caccccgcca cgagggcggg gttgcctgaa attccgtcac ccgggcgagc
     1201 ccgagcgcgg aaaagcctgc gattgtagca gttgcaggcg aacccgcaac tgccgaccgg
     1261 cgtggcgcgg gttactgcag cttgatttcg acgtccacgc cggcgggcag gtcgagcttc
     1321 atcagcgcgt cgacggtctt gtcggtcggg tcgacgatgt ccatcaggcg ctgatgggtg
     1381 cggatctcga actggtcgcg cgacgacttg ttgacgtgcg gcgagcgcag gatgtcgaaa
     1441 cgttgcatgc gcgtcggcag gggcaccggg cccttgacga tcgcgccggt gcgtttggcg
     1501 gtgtcgacga tctcgagcgc cgactggtcg atcagcttgt agtcgaatgc cttgaggcgg
     1561 atgcggatct tttgcttttg catgacggtt ccttaaagag cgttgcggcc ggccttggaa
     1621 gcctccaggc cgcggtgtgt tgtagaggcc ggcgagcgcc catgcgctcg ccagtcgtca
     1681 cgcttcactc gatgatcttg gccacgaccc cggcgcccac ggtgcgcccg ccctcgcgga
     1741 tggcaaagcg caggccttcc tccatcgcga tcgggttgat cagcttgacg gtgatgctga
     1801 cgttgtcgcc gggcatgacc atctccttgt ccttgggcag ctccaccgca ccggtcacgt
     1861 cggtggtgcg gaagtagaac tgcggccggt agttgttgaa gaacggggtg tggcgcccgc
     1921 cctcttcctt gctcaagacg tagatctcgg cggtgaagtg ggtgtgcggc ttgacgctgc
     1981 cgggcttgca caggacttgc ccgcgctgca cgtcttcgcg cttggtgcct ctgagcagga
     2041 tgcccacgtt gtcgcccgct tggccctggt cgagcagctt cctgaacatc tcgacgccgg
     2101 tgcaggtggt cttttgcgtg ggggtgatgc ccacgatctc gatttcttca ccgaccttga
     2161 tgacgccgcg ctcgactctc ccggtgacca cggtgccgcg cccgctgatg ctgaacacgt
     2221 cttcgacggg catcaggaag gcgccgtcga tggcacgctc gggcgtgggg atgtagctgt
     2281 ccagcgcgtc ggcgagcttc aagatgctgc cttcgccgag ttcgcctttg tcgccttcca
     2341 tggcgagctt ggccgacccg tggatgatgg gggtggcgtc gccggggaat tcgtatttgt
     2401 cgagcagttc gcgcacttcc atctcgacga gctcgaggag ctcggcgtcg tcgaccatgt
     2461 cgcacttgtt catgtagacg atgatgtagg gcacgccgac ctggcgcgcc agcaggatgt
     2521 gctcgcgggt ctggggcatg gggccgtcgg cggcggacac gaccaggatg gcgccgtcca
     2581 tctgggccgc gccggtgatc atgttcttga cgtagtcggc gtggccaggg cagtcgacgt
     2641 gggcgtagtg ccggttcttg gtctcgtatt cgacgtgggc ggtgttgatg gtgatgccgc
     2701 gcgccttttc ttcgggcgcc gcatcgatct ggtcgtaggc cttggcttcg ccgccgaaga
     2761 gcttgctcag cacggtggtg atcgccgccg tcaaggtggt cttgccgtgg tcgacgtggc
     2821 cgatggtccc cacgttcacg tgcggcttgg tccgctcaaa tttgcctttt gccatttgta
     2881 tatctccgtc agagtaaaga gcaatgccag tggtaggttt aaggtttccg tcgaggtgtt
     2941 gattcgcgcg ccactggcaa cgcgcgatac cccgccgaag accggctcgt gtttcttact
     3001 tgccgcgcgc ggtgatgatc gcgtcggcga cgttcttcgg cgcttcgctg tagtgcttga
     3061 attccatcgt gtacgtggcg cggccctgcg acatcgagcg cagcgtggtc gagtagccga
     3121 acatttccga cagcggcact tcggccttga tgatcttgcc gccgcccgga atgtcgtcca
     3181 tgccctgcac catgccgcgg cgtgaggaca ggtcgcccat cacgctgccg gcatagtctt
     3241 ccggcgtctc cacttcgacc gccatcatcg gctcgaggat caccggcgat gccttgcggc
     3301 agccgtcctt gaaaccgagc gacgccgcca tcttgaacgc gttttcgttc gagtcgacct
     3361 cgtggtacga accgaaggtc agcgtgacct tcacgtcgac caccggatag cctgccagca
     3421 cgccggccgg cagcgagtct tcgatgccct tcttgaccgc gggaatgaat tccttcggca
     3481 cgacgccgcc cttggtcgca tcgacgaact cgaagccctt gcccggcgcc tgcggctcta
     3541 gcttgagcac gacttggcc
//
LOCUS       DATMJB010000005         2825 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_413829, whole genome shotgun sequence.
ACCESSION   DATMJB010000005 DATMJB010000000
VERSION     DATMJB010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413829"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="VNU48_00120"
     CDS             complement(<1..516)
                     /locus_tag="VNU48_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWI09712.1"
                     /translation="MNARSNLRAVACAIAATAFLAAGCADMSERQKGTAVGAGVGAGV
                     GAAIGSVTGGKAGTGAVIGGALGAVAGNLWSKRMEDRRNAMEQATRGTNVEVTRTENN
                     ELKLNIPNDISFDTGSAAIKPQLRAVLDPFANSLRDDPSARLMIVGHTDNTGSEAVNN
                     PLSVERAQSVRD"
     gene            complement(651..2441)
                     /locus_tag="VNU48_00125"
     CDS             complement(651..2441)
                     /locus_tag="VNU48_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWI09713.1"
                     /translation="MAAPSRADAPAPGRNVRSLGGLLPFLRPYRTKIALAIVFLVLAA
                     LSTLVFPVAMKSLIDQGFVAADPGTRVMALREHFFALFAVGAALGLFSALRFYMVTWL
                     GERVTADLRNAVYAHVVTQSPEFFETNQTGEVLSRLTTDTTLVQTVVGSSLSMGLRNA
                     VLGLGAMVMLIITNPYVMTQVLGILVIVVLPSLYFGRRVRKLSRASQDRVADSSAIAA
                     EVLNAIPVVQSYTQEAREAKRFDVSTENAFATAVKRTRMRAFLVAFIITATFGALLWG
                     LYQGTQAVERGNISAGHLGQTVVYVIILVGSVAVLSEVYGDLLRAAGATERLMELLNL
                     NSPITSPPRPVPLAPTRGGSAVALRDVTFRYPSRPQLATLSHFDLQVAPGETVALVGP
                     SGAGKSTVFQLLLRFYDVAAGEVTLDGVPVRNVALQALRERIGIVPQDSVIFSADAME
                     NIRYGRPSASDAEVVAAATAAFAHDFISALPEGYQTFLGERGVRLSGGQRQRISIARA
                     MLKNPPLLLLDEATSALDAESERMVQAALESAMKDRTTLVIAHRLATVQRADRIVVME
                     AGEIVETGTHAQLVASGCLYARLAALQFDR"
     gene            2598..>2825
                     /locus_tag="VNU48_00130"
     CDS             2598..>2825
                     /locus_tag="VNU48_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWI09714.1"
                     /translation="MARTAPPADFYRAADYCAEESVGYLMKRIMMSIVSQVDKRLDLH
                     GLTSAQWGPLMRLQTVEGATVAELARWLNIDA"
BASE COUNT          425 a          990 c          992 g          418 t
ORIGIN      
        1 gtcgcgcacg ctctgcgcgc gctcgaccga cagcgggttg ttgacggcct cggagccggt
       61 gttgtcggtg tggccgacga tcatcagccg cgcgctcggg tcgtcgcgca ggctgttcgc
      121 gaacgggtcc agcaccgcgc gaagttgcgg cttgatcgcg gcgctgccgg tgtcgaacga
      181 gatgtcgttc gggatgttga gcttgagctc gttgttctcg gtgcgcgtga cctcgacgtt
      241 ggtgccgcgc gtggcctgct ccatcgcgtt gcgccggtct tccatgcgct tggaccacag
      301 gttgccggcc accgcgccga gcgcgccgcc gatcaccgcg ccggtgccgg ctttcccgcc
      361 agtgaccgag ccgatcgccg caccgacgcc ggcgcccaca ccggcaccga cggccgtgcc
      421 cttctggcgc tcgctcatgt cggcgcagcc ggcggcgagg aaggcggtcg ccgcgattgc
      481 gcaggcgacc gcgcgcagat tcgatcgagc gttcatgtgg tgatcctccg aagatggcgc
      541 ggcgcgcggg ttgcgcgcag ggcctgcgcg gattctcgcg agcgggcgct tccgtcggtg
      601 tcggaggggt gcaacgcgag gtgtcagttg aaacctacag cgccgcgcgc tcagcggtcg
      661 aactgcagcg ccgcgagccg cgcgtagagg cagcccgagg ccaccagctg cgcatgcgtg
      721 ccggtctcga cgatctcgcc cgcctccatc acgacgatgc ggtcggcgcg ctgcacggtc
      781 gccaggcggt gcgcgatcac cagcgtcgtg cggtccttca tcgccgactc gagcgcggcc
      841 tgcaccatgc gctcgctctc ggcgtcgagc gcgctggtgg cctcgtcgag cagcagcagc
      901 ggcgggttct tcagcatcgc gcgcgcgatc gagatgcgct ggcgctgccc gcccgacagg
      961 cgcacgccgc gctcgcccag aaaggtctgg tagccctcgg gcagcgcgct gatgaagtcg
     1021 tgcgcgaacg cagccgtcgc ggccgcgacg acctcggcgt cgctggcgct cgggcgcccg
     1081 tagcggatgt tctccatcgc gtcggccgag aagatcacgc tgtcctgcgg cacgatgccg
     1141 atgcgttcgc gcagcgcctg cagcgccacg ttgcgcaccg gcacgccgtc gagcgtgacc
     1201 tcgccggcgg cgacgtcgta gaagcgcagc agcagctgga acaccgtgct cttgccggca
     1261 ccgctgggcc cgaccagcgc caccgtttcg cccggcgcca cctgcaggtc gaagtgcgac
     1321 agcgtcgcga gctgcggccg cgacgggtag cggaaggtca cgtcgcgcag cgccacggcg
     1381 gagccgcccc gggtcggcgc cagcgggacc ggccgcggcg gcgaggtgat cggcgagttc
     1441 aggttcagca gctccatcag ccgttcggtc gcgccggcgg cgcgcagcag gtcgccgtag
     1501 acctccgaca gcaccgccac gctgccgacc aggatgatca cgtagaccac cgtctggccc
     1561 aggtggccgg cgctgatgtt gccgcgctcc accgcctgcg tgccctggta caggccccac
     1621 agcagcgcac cgaaggtcgc ggtgatgatg aacgcgacca ggaacgcgcg catccgcgtg
     1681 cgcttcaccg cggtggcgaa cgcgttctcg gtcgagacgt cgaagcgctt cgcctcgcgc
     1741 gcttcctgcg tgtagctctg caccacgggg atcgcgttca gcacctcggc ggcgatcgcg
     1801 ctcgaatcgg cgacgcggtc ctggctggcg cggctgagct tgcgcacgcg ccggccgaag
     1861 tacaggctgg gcagcaccac gatgaccagg atgcccagca cctgggtcat tacgtacggg
     1921 ttcgtgatga tcagcatcac catcgcgccg aggcccagca ccgcgttgcg caggcccatc
     1981 gacaggctcg agccgacgac ggtctgcacc agcgtcgtgt cggtggtcag gcgcgacagc
     2041 acctcgccgg tctggttggt ctcgaagaac tccgggcttt gcgtgaccac gtgggcgtag
     2101 accgcgttgc gcaggtcggc ggtgacgcgc tcgccgagcc aggtcaccat gtagaagcgc
     2161 agcgccgaga acagcccgag cgccgcgccg accgcgaaca gcgcgaagaa gtgctcgcgc
     2221 agcgccatca cccgcgtgcc cgggtcggcc gcgacgaagc cctggtcgat cagcgacttc
     2281 atcgccaccg gaaacaccag cgtgctcagc gccgccagca ccaggaagac gatcgccagc
     2341 gcgatcttcg tgcggtacgg ccgcaggaac ggcagcagcc cgccgagcga gcgaacgttg
     2401 cgcccgggtg ccggggcgtc ggcgcgcgac ggagcggcca tcggtgtgga gaggggctgg
     2461 gcagggctgg acatggcggc agtgtagagg gtcggctccg cggcaccgcc gcaaacccat
     2521 gctctgacag ctaaagcctt gacaattatt gcctaggcat atatagtcac atcatctcat
     2581 ccgaagcgaa catcaccgtg gcccgcaccg ctccccctgc cgacttctac cgcgccgccg
     2641 actactgtgc cgaagagagc gtcggttacc tgatgaagcg catcatgatg tcgatcgtct
     2701 cgcaggtgga caagcggctc gacctgcacg gcctgacgag cgcgcaatgg ggcccgctga
     2761 tgcgcctgca gacggtcgag ggcgccaccg tggccgagct ggcgcgctgg ctcaacatcg
     2821 atgcc
//
LOCUS       DATMJB010000006         4764 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_413137, whole genome shotgun sequence.
ACCESSION   DATMJB010000006 DATMJB010000000
VERSION     DATMJB010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413137"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /gene="tyrS"
                     /locus_tag="VNU48_00135"
     CDS             complement(<1..849)
                     /gene="tyrS"
                     /locus_tag="VNU48_00135"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWI09715.1"
                     /translation="MSLPFAQASAAAPGGPAHPVTDRVRQAMDISLRGCDELLPRSDW
                     LAKLAKSEATGVPLRIKLGLDPTAPDLHLGHTVVLNKMRQLQDLGHKVIFLVGDFTSM
                     IGDPSGRNTTRPPLTREQIDVNAQTYFAQAKLVLDESKTEVRYNSEWSDPLGARGMIR
                     LASRYTVARMMERNDFHDRFKAGTPISVHEFLYPLMQGYDSVALKSDLELGGTDQKFN
                     LLVGRALQSEYGQEPQCILTMPLLEGLDGVEKMSKSKNNYVGITEPANTMYAKILSIS
                     DVQMWKW"
     gene            1083..2465
                     /locus_tag="VNU48_00140"
     CDS             1083..2465
                     /locus_tag="VNU48_00140"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWI09716.1"
                     /translation="MESLDRALASAAARTGRFVSAHPRSLSAAVMLSLAGFGVTAFGI
                     APMAPDAADIPKRLVTETVTPDGIQSQLDALAEHELQLYRTDLTRTSDTADSLLKRLN
                     VSDAGAAQFLRTDPTARKLLEGRGGKMVRVQTDENGALTELIARYAPADAEQLGTQFT
                     RLTIRRITGKLLAMVETAPLATQVRMGSGTVSSTLFAATDDARIPDNVASQLADVFAT
                     DLDFHRQLRKGDTFSVIYEALTADGEPITWGPSAAGRVLAADFVNNGRTYSAVWFKDA
                     NSKGAYYGFDGQSKKRAFLASPMEFSRVTSGFAMRLHPILNSWKQHKGVDYGAPLGTA
                     IRAVGDGTVDFAGWQNGYGNVVEIRHSAQRSTLYAHMSRIDVRRGAHVEQGQHIGAVG
                     MTGWATGPHLHFEVKVGGVQQDPMIMAKASETVALAPGQKAAFAQMAGSLKSQLEVAD
                     TVARSGTYAE"
     gene            2538..3665
                     /locus_tag="VNU48_00145"
     CDS             2538..3665
                     /locus_tag="VNU48_00145"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HWI09717.1"
                     /translation="MTELYIGLMSGTSLDGVDGVLVDFAASGPDLLHVVAHAHRPFAP
                     ALAAELLALNDPGGANELHRAALAGNALARGYAEVIEAVLTASGTPRDRVRAVGAHGQ
                     TVRHRPREFDGTGYTLQLNSPALLAELCGIDVVADLRSRDLAAGGQGAPLASGFHRAM
                     FGRAGEAVAVLNLGGIGNLTALRADGSTIGFDCGPANALLDHWCRLHTGRAYDDGGAW
                     AASGRVIDPLLERLLTEPYFALPPPKSTGRDLFNPDWLQARLAASDPGAAPVDVQATL
                     AELSAAACAADVQAHAPGARVLLVCGGGAHNADLMRRLAVRLPGVDVMRTDQRGLPAD
                     QVEACAFAWLARACVRRECANVPRVTGAAGPRVLGALYPAR"
     gene            complement(3724..4104)
                     /gene="erpA"
                     /locus_tag="VNU48_00150"
     CDS             complement(3724..4104)
                     /gene="erpA"
                     /locus_tag="VNU48_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HWI09718.1"
                     /translation="MSAVAQNFQSLATMDEPPVPLVFTDSAANKVKELVDEEGNPELK
                     LRVFVQGGGCSGFQYGFTFDEIVNDDDTQMSKNGVTLLIDAMSLQYLAGAEIDYKDDL
                     QGAQFVIKNPNATTTCGCGSSFSA"
     gene            complement(4425..>4764)
                     /gene="rpsI"
                     /locus_tag="VNU48_00155"
     CDS             complement(4425..>4764)
                     /gene="rpsI"
                     /locus_tag="VNU48_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWI09719.1"
                     /translation="VFMKPGKGEITVNGKSVDVFFSRETGRMIVRQPLELTNHAQTFD
                     IKVNVFGGGESGQAGAVRHGITRALMDYDMQLKPVLSKAGLVTRDAREVERKKVGLHG
                     ARRRKQFSKR"
BASE COUNT          725 a         1633 c         1610 g          796 t
ORIGIN      
        1 ccacttccac atctgcacgt cgctgatcga caggatcttg gcgtacatcg tgttggccgg
       61 ctcggtgatg cccacgtagt tgttcttgga cttggacatc ttctcgacgc cgtccagccc
      121 ttccagcagc ggcatcgtca ggatgcactg cggctcctgc ccgtactcgc tttgcagcgc
      181 ccgcccgacc agcaggttga acttctggtc ggtgccgccg agctcgaggt cgcttttcag
      241 ggcgaccgag tcgtagccct gcatcagcgg gtacagaaat tcgtgcacgc tgatcggcgt
      301 gccggccttg aagcggtcgt gaaagtcgtt gcgctccatc atccgcgcca ccgtgtagcg
      361 cgaggcgagc cggatcatgc cccgcgcccc cagcgggtcg ctccactcgg agttgtagcg
      421 gacctcggtc ttgctctcgt cgagcaccag cttggcctgc gcaaaatagg tctgcgcgtt
      481 gacgtcgatc tgctcgcgcg tcagcggcgg gcgcgtcgtg ttgcggcccg acgggtcgcc
      541 aatcatcgag gtgaagtcgc cgaccaggaa gatcaccttg tggcccaggt cctggagctg
      601 gcgcatcttg ttcagcacga ccgtgtggcc caggtgcagg tcgggcgcgg tcggatcgag
      661 gccgagcttg atccgcagtg gcacgccagt tgcttctgac ttggcgagct tggccagcca
      721 gtcggagcgc ggcagcagtt cgtcgcagcc gcgcagcgag atgtccatgg cctgcctgac
      781 ccgatctgtc accgggtggg cggggccccc cggcgcggcg gcagaagctt gagcaaaagg
      841 cagggacata cggggctatt gcggagaggg gcaggggtgc cgggtcgtta tactgcgcgg
      901 cgcgtcgagg cccgctcatc gagggtggag gcggtctgaa cgcgctccgc gcccctgcac
      961 agacaggcgc cacggattct agtagaggcc ctttcgggcg gtagggataa aacaccgaag
     1021 tgccggccac gctgccgtca ctcgcatcag agcggcagcg ctctcgaatc gaattgaaat
     1081 cgttggaatc acttgatcgc gctctggcct cggccgccgc gcgcaccggc cgcttcgtgt
     1141 ccgcccaccc gcgcagcttg tccgccgcgg tgatgctgtc gctcgccggc ttcggcgtga
     1201 ccgcgttcgg cattgcgccg atggcacccg acgccgctga catcccgaaa cgtctcgtga
     1261 ccgaaaccgt cacgccggac ggaattcagt cacaactcga cgcgctggcc gaacacgagc
     1321 tgcagcttta tcgcaccgac ctcacccgca ccagcgacac cgccgacagc ctgctgaaac
     1381 ggctgaacgt gagcgacgcc ggcgcggcgc agttcctgcg caccgacccg accgcgcgca
     1441 agctgctcga gggccgcggc ggcaagatgg tgcgcgtgca gaccgacgag aacggtgcgc
     1501 tgaccgagtt gatcgcacgc tacgcacctg ccgatgccga gcagctcggt acccaattca
     1561 cgcgtctgac gatccgccgg atcacgggca agctgctcgc gatggtcgag accgcgccgc
     1621 tggccactca ggtgcgcatg ggtagtggca cggtgagctc gacgctgttc gcggcgaccg
     1681 acgacgcccg catccccgac aacgtcgcca gccagctcgc cgacgtgttc gccaccgacc
     1741 tcgacttcca ccgccagctg cgcaagggcg acaccttctc ggtgatctac gaggcgctca
     1801 ccgccgacgg cgagccgatc acctggggcc cgtccgcggc cgggcgtgtg ctcgccgccg
     1861 acttcgtcaa caacgggcgc acctattcgg cggtctggtt caaggacgcg aactccaagg
     1921 gcgcgtacta cggcttcgac ggccagagca agaagcgcgc gttcctggcg agcccgatgg
     1981 agttctcgcg cgtcacctcg ggtttcgcga tgcgcctgca cccgatcctg aacagctgga
     2041 agcagcacaa gggcgtcgac tacggcgctc cgctgggcac cgcgatccgc gcggtcggcg
     2101 acggcacggt cgactttgcc ggctggcaga acggttacgg caacgtcgtc gagatccgcc
     2161 acagcgcaca gcgctcgacc ctgtacgcgc acatgagccg gatcgatgtg aggcgcggcg
     2221 cgcatgtcga gcagggccag cacatcggcg cggtcggcat gaccggctgg gccaccggcc
     2281 cgcacctgca cttcgaggtc aaggtcggcg gcgtgcagca agacccgatg atcatggcca
     2341 aggcctcgga aacggtcgcg ctcgcgccgg gccagaaggc ggcgttcgcg cagatggcgg
     2401 gcagcctgaa gagccagctc gaagtcgccg acacggtggc gcgctcggga acgtacgctg
     2461 agtgaagaag aggtgttctc gaacgttcga agggcctgca ttgcgggccc tttctcgttg
     2521 ctgggcacaa tgcttggatg actgagctct acatcggcct gatgtcgggc acctcgctcg
     2581 acggcgtcga cggcgtgctg gtggatttcg ccgcgtccgg ccccgacctg ttgcacgtgg
     2641 tcgcgcacgc gcaccggccg ttcgcgccgg cgctcgccgc agaattgctg gcgctgaacg
     2701 accccggcgg cgcgaacgaa ctgcaccgcg ccgcgctcgc cggcaacgcg ttggcgcgcg
     2761 gctacgccga ggtcatcgag gccgtgctca ccgccagcgg cacgccacgc gaccgggttc
     2821 gcgcggtcgg cgcgcacggc cagaccgtgc gccaccggcc gcgcgagttc gacggcaccg
     2881 gctacacgct gcagctgaac agcccggcgc tgctggccga gttgtgcggc atcgacgtgg
     2941 ttgccgacct gcgcagccgc gacctcgcgg ccggcgggca gggtgcgcca ttggcgtcgg
     3001 gttttcaccg cgccatgttc ggccgcgccg gcgaggcggt ggcagtgctc aacctcggcg
     3061 gcatcggcaa cctgacggcg ctgcgcgccg atggttcgac gatcggcttc gattgcgggc
     3121 ccgccaacgc actgctggac cattggtgcc gcctgcacac cggccgcgcc tatgacgacg
     3181 gcggcgcctg ggcggcgtcc ggccgcgtga tcgatccgct gctcgaacgg ctgctgaccg
     3241 aaccgtactt cgcgctgccg ccgccgaaga gcaccggccg cgatctgttc aaccccgact
     3301 ggctgcaggc ccgcctcgcg gcatccgacc cgggcgcggc accggtcgac gtgcaggcca
     3361 cgctggccga actcagcgcc gcggcgtgcg ccgccgacgt gcaggcgcat gcgccgggcg
     3421 cgcgcgtgct gctggtgtgc ggtggcggcg cccacaacgc cgatctgatg cggcggctcg
     3481 cggtgcggtt gccgggcgtc gacgtgatgc gcaccgacca gcgcgggctc ccggccgatc
     3541 aggtcgaggc ctgcgcgttc gcatggctgg cgcgcgcctg cgtgcggcgc gagtgcgcca
     3601 acgtgccgag agtgaccggc gccgccggac cgcgcgtgct gggcgccttg tacccggcac
     3661 gctgatcatt ccgcgcgcgc aacaaaaaag ccgcccgaag gcggctttcg tggcagcaac
     3721 tgctcaggcc gagaagctcg aaccgcaacc gcaggtggtc gtggcgttcg gattcttgat
     3781 cacgaactgg gcgccttgca gatcgtcctt gtagtcgatc tcggcgccgg ccaggtactg
     3841 caggctcatc gcgtcgatca gcagcgtgac gccgttcttg ctcatctgcg tgtcgtcgtc
     3901 gttgacgatc tcgtcgaagg tgaatccgta ctggaagccc gagcagccac cgccctgcac
     3961 gaagactcgc agcttcagct cggggttgcc ttcctcgtcg accagctcct tgaccttgtt
     4021 ggcggcgctg tcggtgaaga ccagcggcac gggcggctcg tccatcgtgg ccagcgactg
     4081 aaaattttgg gcgactgcac tcatcgtgaa ctcctgcggc ttgcatgccg atctgtccat
     4141 tatcctggat tcgacgcggc gagtttggcg gcgggccttt cggcgcgctc attggtctcg
     4201 attcttcacc atcaatggtg gcgcccggat gcatttcaag cgcctcgcgc cgctcggcct
     4261 gagcgaatcg tgccgttcgc cgccttgtcg gcgatcaggc gccaggttgt gaccttgccg
     4321 atgacgtggg cttcctcgga ctctgatgag gcagtcatgc caggcgcaca aacgcacgaa
     4381 agccacccga aggtggcttt catcggggcg cgcgcgcggc ccgatcagcg cttgctgaac
     4441 tgcttgcggc ggcgtgcgcc gtgcaggccg accttcttgc gctcgacctc gcgcgcgtcg
     4501 cgcgtgacca gccccgcctt cgacaggacc ggcttgagct gcatgtcgta gtccatcagc
     4561 gcgcgcgtga tgccgtggcg caccgcgccg gcctggccgg actcgccgcc gccgaacacg
     4621 ttcaccttga tgtcgaatgt ctgcgcatgg ttggtgagct cgaggggctg gcgcacgatc
     4681 atgcggccgg tctcgcgcga gaagaacacg tcgacggact tgccgttcac cgtgatctcg
     4741 cccttgccgg gcttcatgaa gacc
//
LOCUS       DATMJB010000007         4157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_886725, whole genome shotgun sequence.
ACCESSION   DATMJB010000007 DATMJB010000000
VERSION     DATMJB010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4157
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886725"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VNU48_00160"
     CDS             complement(<1..817)
                     /locus_tag="VNU48_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HWI09720.1"
                     /translation="MFETVYVGMTGLDSYAKGLTVISNNVANLNTPGFKGSQLQFADL
                     FYKNSNAGLGADNPQEQVGAGVGTGGTFLNFRQGEARSTGNDLDLLIDGAGLFVLRKD
                     GETVYSRAGQFEFDKDGYLVDQTTKARVASLDAGQLGDLSITGLRASPPKATTKLTFS
                     GNLSTADSQHVISDVTVYDSLGKAIKLKLTFDNTNATTPGSWKISAATSDGASVGTGE
                     IRFTSGKPTVGFDSVTLNYAPTDGQALSFALDFSSDTTSYSAGTDSTLAAGVQD"
     gene            complement(826..1227)
                     /locus_tag="VNU48_00165"
     CDS             complement(826..1227)
                     /locus_tag="VNU48_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HWI09721.1"
                     /translation="MAIESIGQTATSTTSQSSLGQEDFLKILTTQLNYQDPLKPMDNQ
                     QFMAQMAQFASLEQTRAMNDNIETLLTIQSATQSVGLLGRTVEVSTDSDAAVGEVTSL
                     RFSNGQPLLTVKTAAGQTLTDLSVSKIVIVR"
     gene            complement(1261..1929)
                     /locus_tag="VNU48_00170"
     CDS             complement(1261..1929)
                     /locus_tag="VNU48_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09722.1"
                     /translation="MTDMRVGGSAALPQKGIASASAAPDRQAAWLLQLERARWQAQPR
                     YQNAENADLGTPGPADQEDAAHQEQVAGTNAAPAAGAEAPAAPAVPQVASAEAPPWPT
                     AALLASREAGATNSFSHTALPIRPGAAAPSPDADAAPGVTPALSEAPAADAALPEWQQ
                     RSVHVFVGEEATTVWVRDARLQPDGVARLLKSLRSLLDRGADGHAPVRLTVNGQRVDD
                     PSRR"
     gene            complement(1926..2393)
                     /locus_tag="VNU48_00175"
     CDS             complement(1926..2393)
                     /locus_tag="VNU48_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09723.1"
                     /translation="MTGAPRFRYPLESVLRKHEWDVEALALEHASASKALRENEAEQA
                     RLRAVLHDTNAGLARLRAAGANLDLARQRLLLDYRAAQEEAALAQQAQVQVAQSLCEQ
                     IAQQLFRARQSVKAFENHRTDLHDAHERDARVLAAKEADDAWLMGRRFEDAAP"
     gene            complement(2390..3706)
                     /locus_tag="VNU48_00180"
     CDS             complement(2390..3706)
                     /locus_tag="VNU48_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliI/YscN family ATPase"
                     /protein_id="HWI09724.1"
                     /translation="MQAYAKAIRASDTVRRIGKVVRLSGLAIEAVGPDCAVGDVCELL
                     SKRSGLRLKAEVVALQQGRVVLMPYGELAGIGLDCDVVVSGTTSSLRVGAGLLGRVID
                     AFGQPLDGRPLPPLAEARALKGEPLNPLGRPPIQQVLETGVRCIDALLTVGRGQRMGI
                     FAGSGVGKSTLLGMVARNMRADVNVIGLIGERGREVREFIDKSLGPAGLARSVVVVAT
                     SDQPAAVRTRAAYAATTIAEHFRDAGLDVMLTMDSVTRFAMARREIGLSVGEPPTARG
                     YTPSVFAELPRLLERCGTSDSGGSITALYTVLVEGDDLNDPIADALRSILDGHLVMSR
                     QLANHGHYPAIDVLHSVSRLLNDLASPQEQQLARAAVALLSAYEKNHQMIEIGAYRAG
                     SNPQLDRAMRLVPELNCWLQQETGKPVARAEALQALTALLGPEKRK"
     gene            complement(3706..>4157)
                     /locus_tag="VNU48_00185"
     CDS             complement(3706..>4157)
                     /locus_tag="VNU48_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HWI09725.1"
                     /translation="AQAAYREKLDHLDGLINAAGQAFATQIEGLEDIAVGIAFESLVK
                     LLGDALVTPAGAHAAVSQVLQRAKDHEKLVVHLSPSDFFLLLQQRAQSPALDRPGIEL
                     VPDERVELGGCLVETGAGTLDGRIEAQMDVLRQVLLQVRSDSVAKGG"
BASE COUNT          594 a         1459 c         1394 g          710 t
ORIGIN      
        1 cgtcctgcac acccgcggcc aaggtcgaat cggtgccggc cgagtacgag gtggtgtcgc
       61 tcgagaagtc gagcgcgaag ctcagcgctt ggccgtccgt tggcgcgtag ttcagcgtca
      121 ccgagtcgaa gcccaccgtc ggcttgccgc tggtgaagcg gatttcgccg gtcccgacgc
      181 ttgcgccgtc gctggtcgct gcgctgatct tccagctgcc tggcgtcgtg gcgttggtat
      241 tgtcgaacgt cagcttcagc ttgattgcct tgcccagcga gtcgtagacc gtcacgtcgc
      301 tgatgacgtg ctggctgtcg gccgtggaca ggtttccgct gaacgtcagc ttcgtcgtcg
      361 ctttgggcgg gctggcgcgc agtccggtga tcgagaggtc gccgagctga ccggcgtcca
      421 ggctggccac gcgcgccttg gtggtctggt cgacgaggta gccgtccttg tcgaactcga
      481 actggccggc gcgcgagtag acggtctcgc cgtccttgcg cagcacgaac aggccggcgc
      541 cgtcgatcag caggtcgagg tcgttgccgg tggagcgggc ttcgccctgg cggaagttga
      601 gaaaggttcc gccggtgccc acgccggcgc cgacctgctc ttgtgggttg tccgctccga
      661 gcccggcgtt gctgttcttg tagaacagat cggcgaactg gagctgcgag cccttgaaac
      721 cgggcgtgtt gagattggcg acgttattgc tgatgacggt caggcccttg gcgtagctgt
      781 ccagccccgt catacccaca tagactgttt cgaacatgcc ggctcctatc gaacgatgac
      841 gatcttgctg acgctcaggt cggtcagcgt ctggcccgct gccgtcttga cggtcagcaa
      901 gggctgcccg ttgctgaagc gcaggctggt cacttcgccg accgccgcgt cggagtcggt
      961 cgacacttcg acggtccgcc cgagcaagcc gaccgactgc gtcgccgact ggattgtcag
     1021 caaggtctcg atgttgtcgt tcatcgcgcg cgtttgctcg agcgacgcga actgcgccat
     1081 ctgggccatg aactgctggt tgtccatggg cttgagcggg tcctggtagt tcagctgggt
     1141 cgtgaggatc ttcaggaagt cctcctgccc cagcgacgac tgcgacgtgg tcgacgtcgc
     1201 cgtctgcccg atcgattcaa tggccatgcc gacttctcct cgagagtgtt gaatgggttt
     1261 tcatcgacgg ctcgggtcgt cgacgcgctg cccgttgacg gtcaaccgga ccggtgcatg
     1321 accgtcggcg cctcggtcca gcagcgaacg cagggacttc agcagccgtg caacaccatc
     1381 gggctgcagt cgcgcatcgc gcacccacac ggtggtcgcc tcttcgccca cgaacacatg
     1441 caccgagcgc tgctgccact cgggcagcgc cgcgtccgca gccggcgcct cagacagcgc
     1501 cggtgtcacg cctggcgcgg catcggcatc cggcgaaggc gccgccgcac caggccgtat
     1561 cggcaaggcc gtgtgcgaga acgaattcgt cgcgccagcc tcgcggctgg ccagcaaggc
     1621 ggcggtcggc caaggcggtg cctcggccga tgcgacctgc ggcaccgccg gcgcggccgg
     1681 cgcttcggcg cccgctgccg gcgctgcgtt cgtaccggca acctgttcct gatgcgctgc
     1741 gtcttcttga tccgccgggc cgggcgttcc aaggtctgcg ttctctgcgt tctgatagcg
     1801 cggctgcgcc tgccagcgcg cgcgctccag ttgcagcagc caggccgctt gccgatcggg
     1861 cgcggccgat gcggacgcga tgcccttctg cggcagcgcc gccgacccgc ccacacgcat
     1921 gtcggtcatg gcgcggcgtc ctcgaaacgc cgccccatca gccaggcgtc gtcggcctcc
     1981 ttggccgcca gcacgcgcgc atcgcgctca tgcgcgtcgt gaagatcggt ccgatggttc
     2041 tcgaacgcct tcaccgattg gcgggcacgg aacagttgct gcgcgatctg ctcgcacagc
     2101 gattgcgcca cctgcacctg cgcctgttgg gccagcgcgg cctcctcctg cgccgcgcgg
     2161 taatcgagca gcaagcgctg gcgcgccagg tcgaggttgg cgcctgccgc gcgcaggcgt
     2221 gccaggccgg catttgtgtc gtgcagcacg gcccgcagcc gcgcctgctc ggcttcgttc
     2281 tcgcgcaagg ccttactggc gctggcatgc tcgagcgcca gcgcttcgac gtcccattcg
     2341 tgcttgcgca acacagactc cagcggatag cggaagcgcg gtgcgccggt catttgcgct
     2401 tctccgggcc cagcagggcc gtcaaggctt gaagcgcctc ggcgcgcgcg accggtttgc
     2461 cggtctcctg ctgcagccag cagttcagtt cgggcaccag gcgcatcgcg cggtcgagct
     2521 gtggattgct gccggcacgg taggcgccga tctcgatcat ctggtggttc ttctcgtagg
     2581 cgctgagcag cgccaccgcc gcgcgcgcca gctgctgctc ctgtggcgat gccaggtcgt
     2641 tcagcaggcg actgacgctg tgcaacacgt cgatggccgg gtagtggccg tggttggcaa
     2701 gctgccgcga catgacgagg tggccgtcca gaatcgaacg cagcgcgtcg gcgatcggat
     2761 cgttgaggtc gtcgccctcc accagcaccg tgtacagcgc ggtgatcgag ccgccgctgt
     2821 cggacgtgcc gcagcgctcc agcaagcgcg gcagttcggc aaagaccgag ggcgtgtagc
     2881 cccgcgccgt cggcggctcg ccgaccgaca ggccgatctc gcgcctggcc atcgcgaaac
     2941 gggtgaccga atccatcgtc agcatcacgt ccaggccagc gtcgcgaaag tgctccgcga
     3001 tcgtcgtcgc cgcataggcg gcgcgggtgc gcaccgccgc cggctggtcg gaggtggcca
     3061 ccacgaccac cgagcgcgcg agaccggcag gcccgaggct cttgtctatg aactcacgca
     3121 cctcgcggcc gcgctcgccg atcagcccga tcacgttgac gtcggcgcgc atgttgcgcg
     3181 cgaccatgcc gagcagcgtg ctcttgccca cgccgctccc ggcgaaaatg cccatgcgct
     3241 ggccgcggcc gacggtcaac agcgcatcga tgcagcgcac gcccgtctcc aagacctgct
     3301 ggatcggcgg ccggccgagc gggttcagcg gctcgccctt cagcgcgcgg gcttcggcga
     3361 gcggcggcag cggccggccg tccagcggct gcccgaaggc gtcgatcacc ctgccgagca
     3421 gcccggcgcc gacgcgcagc gaggacgtcg tgccgctcac gacgacatcg cagtccaggc
     3481 cgatgccggc cagttcgccg taaggcatca gcaccacgcg accttgctgc agcgccacga
     3541 cttcggcctt caggcgcagg ccgctgcgct tcgagagcag ttcgcagaca tcgcccacgg
     3601 cgcaatccgg tccgacggcc tcgatggcga gacccgacag tcgcacgacc ttgccgatgc
     3661 gccgcaccgt gtcgctcgcg cgaatcgcct tggcgtacgc ctgcatcacc cgcccttggc
     3721 cacgctatcg ctgcgcactt gcagcagcac ctggcgcagc acgtccatct gcgcttcgat
     3781 gcggccatcc agcgtgccgg cccccgtctc gaccaggcag ccgcccaact cgacgcgctc
     3841 gtcgggcacc agctcgatgc cgggccggtc cagcgcgggc gactgcgcgc gctgctgcag
     3901 cagcaggaag aagtcggacg gcgacagatg gaccacgagc ttctcatggt ccttggcccg
     3961 ctgcagcact tgcgagaccg ccgcgtgcgc gccggccggc gtgaccaacg cgtcgcccag
     4021 cagcttgacc aacgactcga acgcgatgcc caccgcaatg tcttcgaggc cctcgatctg
     4081 cgtcgcgaac gcctggcccg cggcgttgat caggccatcc aggtggtcga gcttctcgcg
     4141 gtaggcggcc tgcgcct
//
LOCUS       DATMJB010000008         5933 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_57321, whole genome shotgun sequence.
ACCESSION   DATMJB010000008 DATMJB010000000
VERSION     DATMJB010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5933
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57321"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /gene="actP"
                     /locus_tag="VNU48_00190"
     CDS             complement(<1..1061)
                     /gene="actP"
                     /locus_tag="VNU48_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430519.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HWI09726.1"
                     /translation="MSRFTDNTRRALTLAALFGVSAAVWAAGADLGQAVKQPTNWTAI
                     SMFAIFVVFTLFITKWAAAKTKSTADFYTAGGGITGFQNGLAIAGDYMSAASFLGISA
                     AVMATGFDGLIYSIGFLVGWPVITFLMAERLRNLGKFTFADVAAFRFKQAPVRIFAAS
                     GTLVVVAFYLIAQMVGAGQLIKLLFGLEYWIAVVIVGSLMMVYVLFGGMTATTWVQII
                     KACMLLGGATFMALSVLWSFGFSPEAMFAGAVKIKTDLALKDGKTLEAATKIGSSIMG
                     PGNFVKDPISAISFGMALMFGTAGLPHILMRFYTVPDARTARVSVSYATAFIGFFYLL
                     TFILGFGAMALVGQAPIKAV"
     gene            complement(1058..1366)
                     /locus_tag="VNU48_00195"
     CDS             complement(1058..1366)
                     /locus_tag="VNU48_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HWI09727.1"
                     /translation="MEQELAQRIASHPKYKELKAKRTRFGWWLTLAMMVVYYGFILLV
                     AFDKEFLSQRLGAGVMTIGMPIGFGVIVFTIVITAIYVRRANSEFDDLSADIAKAVLK
                     "
     gene            complement(1535..2185)
                     /locus_tag="VNU48_00200"
     CDS             complement(1535..2185)
                     /locus_tag="VNU48_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HWI09728.1"
                     /translation="MTIKHLDFWFDPISPFGYLAFERLPQVLEGLSFDVSYRPVLFAG
                     LLKHWGQKGPAEIEPKRAWTFRHAHWIAAQHGIEMATPAQHPFNPLAHLRLLLACAPA
                     RGTPNRRACEAVLRHIWQGGLDANDGARMAALTRELAPRVAPDDAAIRQALKDSTDAA
                     LALGVFGVPTTVVDGRLFWGVDALPMLAAFLRGDPWFDGGAWAAEGAARPGVRRRA"
     gene            complement(2182..2388)
                     /locus_tag="VNU48_00205"
     CDS             complement(2182..2388)
                     /locus_tag="VNU48_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HWI09729.1"
                     /translation="MPPVDSSVASPCNSVCRMDAATGWCEGCLRTIDEIVAWATLDDA
                     AKLAVWDRLAQRRMEAPFLAPERR"
     gene            complement(2388..2879)
                     /locus_tag="VNU48_00210"
     CDS             complement(2388..2879)
                     /locus_tag="VNU48_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HWI09730.1"
                     /translation="MTTDQPDRPEGFRRVSAVLAQRGHAHVPLFLEVAARTAQEAADA
                     LGVGVGQIAKSVVFRRKSDDAAVLVVTSGDKRVDEKKVAALIGGVGRADAEFVKAKTG
                     FTIGGVAPVGHATEPVLLIDRELFRFDEIWAAAGHPNGVFRLSPAALAQLTGAPVEDV
                     VQI"
     gene            complement(2876..3814)
                     /locus_tag="VNU48_00215"
     CDS             complement(2876..3814)
                     /locus_tag="VNU48_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HWI09731.1"
                     /translation="MTLPSHVTLVEVGPRDGLQNEKLPVDAATKIELVHRLQAAGLRE
                     IEVTSYVSPKWVPQMADNAQVMSGVRRRPGVRYSVLTPNLKGFEAAVKDPLIRPDEIV
                     VFASASEAFSRRNINCSIEESVERFRPVVAAAHAQRIKVRSAVSVALGCPYQGEVTPD
                     EVEHVVRLMKAIGVDHCGIADTIGVGTPRRTQAAMERALKHFPLAEVSGHFHDTYGQA
                     LANIYACLELGIHTFDASVAGLGGCPYAKGATGNVGTEDVLFMLNGLGIETGVDIDQL
                     VDAGAFISGVLGRKPVSRSGNALLAKRAQAAAALPA"
     gene            complement(3885..5888)
                     /locus_tag="VNU48_00220"
     CDS             complement(3885..5888)
                     /locus_tag="VNU48_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HWI09732.1"
                     /translation="MFKKILIANRGEIACRVIKTARRMGIATVAVYSEADANARHVRL
                     ADEAVLIGPAAARESYLVADRILAAARQTGAEAIHPGYGFLSENADFAEACERAGIVF
                     IGPPASAIRAMGSKSAAKALMEKAAVPLTPGYHGDRQAAGFLAVQADRIGYPVLIKAS
                     AGGGGKGMRIVTKSDEFDAALASCKREAINSFGIEAVLIERYVTRPRHIEIQVFGDAH
                     GNCVYLFERDCSVQRRHQKVLEEAPAPGMSEARRAEMGAAAVAAAKAVGYVGAGTVEF
                     IAEPTADGDLNFFFMEMNTRLQVEHPVTEAITGLDLVEWQLRVACGEPLPTQQHELRI
                     HGHAIEARICAENPDSNFLPATGRLEVYRTPAGALSFERGNVRVDAGVREGDAVLPFY
                     DSMIAKLIVWGADRAQALARLDAALAATHIVGLHTNVAFLRRVVASASFANADLDTAL
                     IERERDVLFNAAPLTIEVAAAGVVAHALAAERALESSDPWSRRDGWRLHGSAMRRFDL
                     MAQGTHHVFTLERNAGAQTLVAGARRWPLATRALAPNRYDVSLGERRLHLTVYATGER
                     FSVFAPDGSAVLDEFDPIAHSADGAVEGGGLAAPMPGKVIAFLAKAGDTVTQGQPLAV
                     MEAMKMEHTIAAPRDGTIAELLYAVGDQVAEGGELLRMAPAAA"
BASE COUNT          901 a         2090 c         2057 g          885 t
ORIGIN      
        1 accgccttga tcggcgcctg cccgacgagc gccatcgcac cgaagccgag gatgaaggtc
       61 agcaggtaga agaagccgat gaacgccgtc gcgtagctga ccgagacgcg ggcggtccgc
      121 gcgtcgggca cggtgtagaa gcgcatcagg atgtgcggca gcccggcggt gccgaacatc
      181 agcgccatgc cgaacgagat cgccgagatc gggtccttga cgaagttgcc cgggcccatg
      241 atcgacgagc cgatcttggt cgcggcttcc agcgtcttgc cgtccttcag cgcgaggtcg
      301 gtcttgatct tgaccgcgcc ggcgaacatc gcctcggggc tgaagccgaa ggaccacagc
      361 accgacagcg ccatgaaggt cgcgccgccg agcagcatgc aggccttgat gatctgcacc
      421 caggtggtgg ccgtcatgcc gccgaacagc acgtagacca tcatcagcga gccgacgatc
      481 acgaccgcga tccagtactc caggccgaac agcagcttga tcaactggcc cgcaccgacc
      541 atctgcgcga tcaggtagaa cgccacgacg accagcgtgc ccgaggccgc gaagatgcgc
      601 accggcgcct gcttgaagcg gaacgcggcg acgtcggcga aggtgaactt gcccaggttg
      661 cgcaaccgct cggccatcag gaaggtgatc accggccagc cgaccaggaa gccgatcgaa
      721 tagatcaggc cgtcgaagcc ggtggccatc accgccgccg agatgccgag gaacgaggcc
      781 gccgacatgt agtcgccggc gatcgccagg ccgttctgga acccggtgat gccgccgccc
      841 gcggtgtaga agtcggcggt cgacttggtc ttggccgcgg cccacttggt gatgaacagc
      901 gtgaacacga cgaagatcgc gaacatgctg atcgcggtcc agttggtcgg ctgcttgacc
      961 gcctggccga ggtcggcgcc ggccgcccag accgccgcag aaacgccgaa cagcgcggcc
     1021 agcgtcagcg cgcggcgggt gttgtctgtg aagcggctca tttcagcacc gccttcgcga
     1081 tgtcggccga caggtcatcg aattcgctgt tcgcacgccg cacgtagatc gcggtgatca
     1141 cgatcgtgaa cacgatgacg ccgaagccga tcggcatgcc gatcgtcatc acgccggcgc
     1201 ccaggcgctg cgacaggaac tccttgtcga aggccaccag caggatgaag ccgtagtaca
     1261 cgaccatcat cgccagcgtc agccaccagc cgaagcgcgt tcgcttggcc ttcagctcct
     1321 tgtatttcgg atggctcgcg atgcgctggg ccagttcctg ttccatgatg tctcctggtt
     1381 gttgtgatgg ggtgcgcgca ggcgtggatg gtgcgcgcgg gcgctgacct cggactgacg
     1441 cgaaccttgg cctgcgctta caacgcgggg gcgaaaacgc ttcggaacgc gcgcccgatc
     1501 gtgccgacct ttcgggcgcc ggcgttgcgc tgcgtcaagc tctgcgtcga acgcccgggc
     1561 gcgcagcgcc ctcggccgcc cacgcgccgc cgtcgaacca ggggtcgccg cgcagaaagg
     1621 cggcgagcat cggcaatgca tcgacgcccc agaacagccg accgtcgacg actgtcgtcg
     1681 gcacgccgaa gacaccgagc gcgagcgcgg cgtcggtgct gtccttcagc gcctgcctga
     1741 tcgccgcgtc gtcgggcgcg acacgcggcg cgagttcccg cgtcaacgcg gccatgcgcg
     1801 cgccgtcgtt cgcatccagc ccgccctgcc agatgtggcg cagcaccgcc tcgcaggcgc
     1861 gccggttcgg cgtgccgcgc gcaggcgcgc aggccagcag caggcgcaaa tgcgcgagtg
     1921 ggttgaacgg gtgctgcgcc ggcgtcgcca tctcgatgcc gtgctgcgcg gcgatccagt
     1981 gcgcgtggcg gaaggtccag gcacgcttcg gctcgatctc ggccgggccc ttctggcccc
     2041 agtgcttcag caggccggcg aacagcaccg ggcggtagct cacgtcgaac gagagcccct
     2101 ccagcacctg cggcaggcgc tcgaacgcga ggtacccgaa cggcgatatc gggtcgaacc
     2161 agaagtcgag gtgcttgatc gtcatcggcg ctccggcgcc agaaacgggg cctccatgcg
     2221 gcgttgtgcg agccggtccc agaccgcgag cttcgcggcg tcgtccagcg tggcccaggc
     2281 gacgatctcg tcgatcgtgc gcaggcaccc ttcgcaccag ccggtggcgg cgtccatccg
     2341 gcacacgctg ttgcaaggcg acgcgacgct gctgtcgacc ggcggcatca gatctgaacc
     2401 acgtcttcga ccggtgcgcc ggtcagttgc gccagcgcgg ccgggctgag ccggaacacg
     2461 ccgttcgggt ggccggccgc ggcccagatc tcgtcgaagc ggaacagctc gcggtcgatc
     2521 agcagcaccg gctcggtcgc gtggccgacc ggcgccacac caccgatcgt gaaaccggtc
     2581 ttggccttga cgaactcggc gtcggcgcgg cccacgccgc cgatcagcgc ggcgaccttc
     2641 ttctcgtcga cgcgcttgtc gcccgaggtc acgaccagca ccgccgcgtc gtcgctcttg
     2701 cgccggaaca ccacgctctt cgcgatctgg ccgacgccca cgccgagcgc gtcggcggcc
     2761 tcctgcgcgg tgcgcgcggc gacctcgagg aagaggggca catgcgcgtg gccgcgttgt
     2821 gccagcaccg cgctgacgcg gcgaaagcct tcgggccgat ccggctgatc ggtcgtcatg
     2881 cgggcagcgc ggcggcggcc tgcgcgcgct tggcgagcag cgcattgccc gagcgcgaca
     2941 ccggcttgcg cccgagcacg ccggagatga acgcgcccgc gtcgaccagc tggtcgatgt
     3001 cgacgccggt ctcgatgccg aggccgttca gcatgaacag cacgtcctcg gtgccgacgt
     3061 tgccggtcgc gcccttcgcg tacgggcagc cgccgagacc ggcgacgctg gcatcgaagg
     3121 tgtggatgcc cagctccagg caggcgtaga tgttcgcgag tgcctgcccg taggtgtcgt
     3181 ggaaatggcc gctgacctcg gcgagcggga agtgcttcag cgcgcgttcc atcgccgcct
     3241 gcgtgcgccg cggcgtgccc acgccgatcg tgtcggcgat gccgcagtgg tcgacgccga
     3301 tggccttcat cagccgcacc acgtgctcga cctcgtcggg cgtgacctcg ccctggtacg
     3361 ggcagccgag cgcgaccgag accgcgctgc gcaccttgat tcgctgcgcg tgcgccgccg
     3421 cgacgaccgg ccggaagcgc tcgacgctct cctcgatgct gcagttgatg ttgcgccggc
     3481 tgaaggcctc gctggcggac gcgaacacga cgatctcgtc ggggcggatc aaagggtctt
     3541 tgaccgccgc ctcgaagccc ttcaggttcg gcgtcagcac cgaatagcgc acgccgggcc
     3601 ggcgccgcac gccgctcatc acctgcgcgt tgtcggccat ctgcggcacc cacttcgggc
     3661 tgacataact ggtgacctcg atctcgcgca ggccggccgc ctgcaggcga tgcacgagtt
     3721 cgatcttggt cgccgcgtcg accggcaact tttcgttctg caggccgtcg cgcgggccga
     3781 cctcgacgag cgtgacgtgg gaagggaggg tcatcgcaag cgcctcacga gctggacttc
     3841 gcggcgattt tgcgctgctt cgcggccccg gggcggcgcc ggggtcatgc cgccgccggc
     3901 gccatgcgca acagttcgcc gccctcggcg acctggtcgc ccacggcata gagcagctcg
     3961 gcgatcgtgc cgtcgcgcgg cgcggcgatc gtgtgctcca tcttcatcgc ctccatcacc
     4021 gccagcggct gaccctgtgt gaccgtgtcg ccggccttgg cgaggaacgc gatcaccttg
     4081 cccggcatcg gcgcggccag gccgccgccc tcgaccgcgc catcggccga atgcgcgatc
     4141 gggtcgaact cgtcgagcac cgccgagccg tcgggcgcga agacgctgaa gcgctcgccg
     4201 gtggcgtaca cggtcagatg gaggcgccgt tcgcccaagc tcacgtcgta gcggttcggc
     4261 gccagcgcac gggtcgcgag cggccagcgc cgtgcgcccg cgaccagcgt ctgcgcgccg
     4321 gcgttgcgct cgagcgtgaa gacatggtgc gtgccctgcg ccatcaggtc gaagcggcgc
     4381 atcgcgctgc cgtgcaggcg ccagccgtcg cggcgcgacc aggggtcgct gctttcgagc
     4441 gcgcgctcgg ccgccagcgc gtgcgcgacg acgccggcgg ccgccacctc gatagtcagc
     4501 ggcgcggcgt tgaacagcac gtcgcgttcg cgttcgatca gcgcggtatc gaggtcggcg
     4561 ttcgcgaacg atgcactcgc gacgacgcgg cgcaggaacg cgacattggt gtgcagcccg
     4621 acgatgtgcg tggccgccag cgccgcgtcc aggcgcgcca gcgcctgcgc gcggtcggcg
     4681 ccccagacga tcagcttggc gatcatcgag tcgtagaacg gcagcaccgc gtcgccttcg
     4741 cgcacgccgg cgtcgacgcg cacgttgccg cgctcgaagc tcagcgcgcc cgccggcgtg
     4801 cgatagacct cgagccggcc ggtcgcgggc aggaagttcg agtccgggtt ctcggcgcag
     4861 atgcgcgcct cgatcgcgtg gccgtggatg cgcagctcgt gctgctgcgt cggcagcggc
     4921 tcgccgcaag cgacgcgcag ctgccactcg accaggtcga gcccggtgat cgcctcggtc
     4981 accgggtgct cgacctgcag ccgcgtgttc atctccatga agaagaagtt caggtcgccg
     5041 tccgcagtcg gctcggcgat gaactcgacc gtgccggcgc cgacatagcc caccgccttc
     5101 gccgccgcga ccgccgcggc gcccatctcg gcacgccgcg cctcgctcat gccgggtgcc
     5161 ggcgcttcct cgagcacctt ctggtggcgg cgctgcaccg agcagtcgcg ctcgaacagg
     5221 tagacgcagt tgccgtgcgc gtcgccgaac acctggatct cgatgtggcg cgggcgcgtg
     5281 acgtagcgct cgatcagcac cgcctcaatg ccgaagctgt tgatcgcctc gcgcttgcac
     5341 gaggcgagcg cggcatcgaa ctcgtcgctc ttcgtgacga tgcgcatgcc cttgccgccg
     5401 ccgccggcgc tggccttgat cagcaccggg tagccgatgc gatcggcctg cacggcgagg
     5461 aagccggccg cctgcctgtc gccgtgatag ccgggcgtca gcggcaccgc ggccttctcc
     5521 atcagcgcct tggcggccga cttcgagccc atcgcgcgga tcgccgaagc gggcgggccg
     5581 atgaagacga tgccggcacg ctcgcaggct tcggcgaagt ccgcgttctc cgacaggaag
     5641 ccgtagccgg ggtggatcgc ctcggcaccg gtctggcggg cggcggcgag gatcctgtcg
     5701 gccaccaggt aggactcgcg tgccgccgcc gggccgatca gcaccgcctc gtcggccagg
     5761 cgcacatggc gggcattggc atcggcctcg gaatagacgg cgacggtggc gatgcccatg
     5821 cggcgcgccg tcttgatgac gcggcaggcg atctcgcccc gattggcgat gaggatcttc
     5881 ttaaacatgg agtgcctcca tcggggcgag atcgcctgca tgcgctgcgc gca
//
LOCUS       DATMJB010000009         2963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_530869, whole genome shotgun sequence.
ACCESSION   DATMJB010000009 DATMJB010000000
VERSION     DATMJB010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530869"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VNU48_00225"
     CDS             complement(<1..699)
                     /locus_tag="VNU48_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS domain-containing protein"
                     /protein_id="HWI09733.1"
                     /translation="MGINTTSFANTPQAGDDFFTAAMTGLTEDSTGVTYLAVMANDLG
                     GAAKTLYSIDDGTTSIGVISPTDLLTQDTVRVESTSGDHSAHGANIWITADGKVGYDA
                     ATLSAAFKDQLQHLAAGQFLTDTFTYAIRLGNGTLSWATATVQIVGVNDAPVITGAVT
                     GSALEDGSIVSLNALANASDVDIGAVLSVTNVPGTLPAGVTYDAATHTFALDPSNAAF
                     QHLAAGAHTTVTVSY"
     gene            1233..2156
                     /locus_tag="VNU48_00230"
     CDS             1233..2156
                     /locus_tag="VNU48_00230"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09734.1"
                     /translation="MGTPVDGEMLGFDPVARILTFRSSAIGPVVELPFSRFRRLTLTV
                     PLQPVVPIAGVPIERVPAAAQEREYRLQSHATAPVLTGRTAGRIETADGLYLFTPVEE
                     EASLQRVFVPRWVYTRCEFGPSAEEVAARMWIASPAELLAAIERQQRMPILPLGQSLL
                     KLGLLTQAQLDRALARQSRDKPLGESLVADGLISRADLQTALAHKMGYPLIDLARFPI
                     DPKAVAKLPPRIAVGFRMMPLMLKGDRLIVAVDKPARVIKLGTVHAIAGLTVVPVLAS
                     RMQILLAQERLSHDIWSGHVAERIGFFATTV"
     gene            complement(2193..>2963)
                     /locus_tag="VNU48_00235"
     CDS             complement(2193..>2963)
                     /locus_tag="VNU48_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWI09735.1"
                     /translation="RFGLIGSLGNWVIEDACRQSRAWRDKGLRMRVAINLSAFQMRQD
                     DLVERITTALARHRIDPSLLTCEITESVAMEDTRATQITFKRLGDAGVHLSIDDFGTG
                     YSSLSYLRKLPAEELKIDRSFVMDIETSADARAVVDAVIHLAHALGLKVVAEGVETER
                     QRDVLIQMGCDELQGYLFAKPMSARALLLWASDDREREVAFKPSLFGETRPVSEQDLL
                     AHRRAATSTVPQAHSKPDSAWSRLIGFADTLPSGSSKL"
BASE COUNT          459 a         1019 c          963 g          522 t
ORIGIN      
        1 gtaactcacg gtcacggtcg tgtgagcgcc ggcagcaaga tgctggaagg ctgcgttcga
       61 tgggtcaagg gcaaaggtgt gcgtcgcggc gtcgtacgtc acgccggccg gcaatgtgcc
      121 gggcacattg gtcaccgaca acaccgcgcc gatatcgaca tcggaagcat ttgccagcgc
      181 gttcaagctg acgatcgagc cgtcctcgag cgcgcttccc gtcaccgcgc ccgtgatcac
      241 cggcgcgtca ttgacgccga cgatctgcac cgtcgcggtg gcccagctga gcgttccgtt
      301 gcccagacgg atcgcatacg tgaacgtgtc cgtgaggaac tggcccgcag cgaggtgctg
      361 caactgatcc ttgaacgctg cggaaagcgt cgctgcgtca tagccaactt tgccgtcggc
      421 cgtgatccag atgttggcgc cgtgggcgct gtggtcaccg ctcgtggact cgaccctgac
      481 cgtgtcctgg gtcaaaagat ccgtcggact gatcacgcca atacttgttg tgccatcgtc
      541 gatcgaatac aacgtcttcg cagcgccgcc caaatcgttg gccatcaccg cgagatatgt
      601 gacgccagtc gaatcctccg tcagtccggt catcgccgcg gtgaagaagt catctccggc
      661 ttgcggcgtg ttggcgaacg aagtggtgtt gatgcccatg gtctgcgctt ccctgtctgt
      721 gttgatgact accggtgatg tctgcgagtg accgactgct gttcctcgcc gcgtcgctca
      781 cttgtgaaca aatgctaagg ggagcgccac gcgagatgtc ccccctgact cggggccgca
      841 taacgcctgg accgtgaagc ccagcacgga acattgcatc gtccacatcg ctgagcgact
      901 gctccggtgg tttcgtggat ccccgtgctt cgacgtcatc gccggcgatg ggtggccccg
      961 ctttttcaac cgagcaaatg catggcgatt cgcgcgtttc ttcacggtct gctgcgcgac
     1021 gcctgatgta tcttgggcgc aaccttgcat cattggccac caccacaggg cgcacccgcg
     1081 tcgtcaccgt cgtcgagctc atgtccaccc catttccaac actgatggaa gtagcgccag
     1141 agcgctggtg ctggcccgtg ccgcccttcg cctggcggca ccgccccgcg cccggcaata
     1201 acgagccgca gccgtgccgc atcgagggcc ccgtgggcac gccggtcgac ggcgagatgc
     1261 tgggcttcga tccggtcgcg cgcatcctca ccttccgcag ctcggccatc ggcccggtcg
     1321 tcgaactgcc gttctcgcgc tttcgccggc tcacgctgac cgtaccgctg cagcccgtcg
     1381 tgccgatcgc cggcgtgccg atcgagcggg ttccggccgc cgcgcaggag cgcgagtatc
     1441 gattgcagtc ccacgccacc gcgcccgtgc tcaccgggcg caccgccggg cgcatcgaga
     1501 ccgccgacgg cctgtacctg ttcacgccgg tcgaagagga agcgtcgctg cagcgcgtct
     1561 tcgtgccgcg ctgggtctac acgcgctgcg agttcggccc gtccgccgag gaggtcgcag
     1621 cgcggatgtg gatcgccagc ccggccgagc tgctcgcggc gatcgagcgc cagcaacgca
     1681 tgccgatcct gccgctgggc cagtcgctgc tcaagctcgg cctgctgacc caggcgcagc
     1741 tcgaccgcgc gctggcgcgg cagtcgcgcg acaagccgct gggcgagtcg ctggtcgccg
     1801 acgggctcat ttcgcgcgcc gacctgcaga ccgcgctggc ccacaagatg ggctacccgc
     1861 tgatcgacct ggcgcgtttt ccgatcgacc cgaaggcggt ggccaagctg ccgccgcgca
     1921 tcgccgtcgg ttttcggatg atgcccttga tgctcaaggg cgatcgcctg atcgtggcgg
     1981 tcgacaagcc ggcgcgcgtg atcaagctcg gcacggtcca tgcgatcgcc ggcctcaccg
     2041 tggtgccggt gctggcctcg cggatgcaga tcctgctggc gcaggaacgg ctctcgcacg
     2101 acatctggtc ggggcacgtc gccgagcgca tcggcttctt cgcgaccacc gtctaacggg
     2161 ccgcgcgccc ggttcgggcg cgaacaacaa ccctagagct tggagctgcc gctgggcagc
     2221 gtgtccgcga agccgatcag gcgcgaccac gcgctgtcgg gcttggaatg ggcctgaggc
     2281 accgtcgacg tcgcggctcg ccgatgtgcc agcaggtcct gctcggacac cgggcgcgtc
     2341 tcgccgaaca gcgaaggctt gaacgcgacc tcgcgctcgc ggtcgtcgct ggcccacagc
     2401 agcagcgcgc gcgccgacat cggcttggcg aacaggtagc cctgcaactc gtcgcagccc
     2461 atctggatca gcacgtcgcg ctgacgctcg gtctcgacac cctcggcgac gaccttcagg
     2521 ccgagcgcgt gcgccaggtg aatcaccgcg tcgacgaccg cgcgcgcatc ggcgctggtc
     2581 tcgatgtcca tcacgaagct gcggtcgatc ttcagttcct cggccggcag cttgcgcagg
     2641 tagctcaggc tcgagtaacc ggtgccgaag tcgtcgatcg acagatgcac gccggcgtca
     2701 cccaggcgct tgaaggtgat ctgggtcgct cgcgtgtctt ccatcgcgac cgactcggtg
     2761 atctcgcagg tcagcagcga cgggtcgatg cgatgccgcg cgagcgcggt cgtgatgcgc
     2821 tcgaccaggt cgtcctggcg catctggaac gccgacaggt tgatcgcgac gcgcatgcgc
     2881 agccccttgt cgcgccaggc gcgcgactgg cggcacgcgt cctcgatgac ccagttgccg
     2941 agtgagccta tcaggccgaa acg
//
LOCUS       DATMJB010000010         2129 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_57099, whole genome shotgun sequence.
ACCESSION   DATMJB010000010 DATMJB010000000
VERSION     DATMJB010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57099"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1160)
                     /locus_tag="VNU48_00240"
     CDS             complement(60..1160)
                     /locus_tag="VNU48_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6352 family protein"
                     /protein_id="HWI09736.1"
                     /translation="MAAHFAHSRPDPPMQDFWPASDYAALDPTPRGWLRPTEAWLRSF
                     LALPELALVDESCAAEIALHRSLLDAPARPVSTAELDALQDDDARANYAMFLAFRDAL
                     LAAGTLEAHYLALMRSARVTIPPLFVDRIVQAIARHLLDAGSDAFEARAAEMLFRAQR
                     LTLADGRLLAADLATADLLNETGGFGELGRLLVQGNAPLAAVEMAVLGPDNAADYWRR
                     SARHAFVLDLTHELANDLGHGLSFRLTRAHSGLKALAAVLQRWVAHLLGVQVAITPLQ
                     RIDDAAWRWHIGLDVDSMALLNDLYEDRPVGAERMGRLISLFRLEFANPAEMRADVAG
                     KPVYLGLAMSADGTLRLKPQNLLLNLPLAASM"
     gene            1255..1791
                     /gene="mobB"
                     /locus_tag="VNU48_00245"
     CDS             1255..1791
                     /gene="mobB"
                     /locus_tag="VNU48_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HWI09737.1"
                     /translation="MNVIGFCGYSGSGKTTLVEQLIARLKRAGLRVSVVKHAHHAFDI
                     DHEGKDSWRHRRAGAYEVVIASDRRLAKVREFETRAAPTVHDLIDELDGCDWVLVEGF
                     KRATLPKIEIWRAATGQPAQYPNDAHIVAVCTDSARALPEGTELPVLDLNDADAVTRF
                     LIDSAERFRYVAPARALR"
     gene            complement(1804..>2129)
                     /locus_tag="VNU48_00250"
     CDS             complement(1804..>2129)
                     /locus_tag="VNU48_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HWI09738.1"
                     /translation="GRLDLGLAISAVTLGLVALAHEVAGGPIGEWLAWGLGFFVVGWV
                     LQFVGHYYEGRKPAFVDDMTGLLVGPMFVTAEAMFMLGWNKPLLAEIERRAGPTVLRD
                     MARIA"
BASE COUNT          339 a          747 c          748 g          295 t
ORIGIN      
        1 cgaggtgctg tcgctgcgcc agatcttgcc gtggtggaac taccgcaagc ccaaaccggc
       61 tacatcgacg cggcgagcgg caggttcagc aacaggttct gcggcttgag tcgcagcgtg
      121 ccgtcggcgc tcattgccag gccgaggtag accggcttgc cggcgacgtc ggcacgcatc
      181 tcggccgggt tcgcgaactc gagccggaac aggctgatca atcgccccat gcgttcggcg
      241 ccgaccggcc ggtcttcgta caggtcgttg agcagcgcca tcgaatcgac gtccagcccg
      301 atgtgccagc gccaggccgc atcgtcgatg cgctgcagcg gcgtgatcgc gacctgcacg
      361 cccagcaggt gcgcgaccca gcgctgcagc accgcggcga gcgccttcaa cccggagtgc
      421 gcgcgcgtca gcctgaagct caggccgtgg cccaggtcgt tcgcgagttc gtgcgtcagg
      481 tcgagcacga acgcgtggcg cgcgctgcgg cgccagtagt cggccgcgtt gtccgggccg
      541 agcaccgcca tctccaccgc ggccagcggc gcgttgccct gcaccagcag ccggccgagt
      601 tcgccgaagc cgccggtttc gttcaacagg tcggcggttg ccaaatcggc ggcgagcagg
      661 cggccgtcgg ccagcgtgag ccgctgcgcc cggaacagca tctcggcggc gcgggcctcg
      721 aacgcgtcgc tgccggcgtc gagcagatgg cgcgcgatcg cctgcacgat gcgatcgacg
      781 aacagcggcg ggatcgtcac gcgagcgctg cgcatcagtg cgaggtaatg ggcttcgagc
      841 gtgccggcgg cgagcagcgc atcgcggaac gcgaggaaca tcgcgtagtt ggcgcgcgca
      901 tcgtcatcct gcaatgcgtc gagttcggcg gtcgataccg gacgcgccgg cgcgtcgagc
      961 agcgatcggt gcagcgcgat ctcggcggcg cacgactcgt cgaccagcgc gagttcgggc
     1021 agcgcgagga aggaccgcag ccaggcctcg gtcggccgca gccagccgcg cggcgtgggg
     1081 tcgagcgcgg cgtagtcgct ggcgggccag aagtcctgca tcggtgggtc ggggcgcgag
     1141 tgcgcgaagt gcgccgccat tatcggagtg tggcccgggg cgtcgcgcat acactgccgg
     1201 cgcgcgagcc gtttgccgcg cccgattccc ctctcgaaag acccacgcgc cacgatgaac
     1261 gtcatcggct tctgcggcta ttccggttcc ggcaagacca cgctggtcga gcaactcatc
     1321 gcgcgcctga agcgcgccgg tctgcgcgtc tcggtcgtca agcacgcgca ccacgcgttc
     1381 gacatcgacc acgagggcaa ggactcgtgg cggcatcgcc gcgccggtgc ctacgaggtc
     1441 gtgatcgcgt cggatcggcg tctggcgaag gtgcgcgagt tcgagacccg cgccgcgccg
     1501 accgtgcacg acctgatcga cgaactcgat ggctgcgact gggtgctggt cgaaggcttc
     1561 aagcgcgcga cgctgccgaa gatcgagatc tggcgcgccg ccaccggcca gcctgcgcag
     1621 tatccgaacg acgcgcacat cgtcgcggtc tgcaccgaca gcgcgcgtgc gctgcccgaa
     1681 ggcaccgagc tgccggtgct ggacctgaac gacgccgacg cggtgacgcg cttcctgatc
     1741 gacagcgcgg agcgcttccg ctacgtcgcg ccggcgcgag ccctccgata gcgttaacag
     1801 gccctaggcg atccgcgcca tgtcgcgcag cacggtcggc ccggcgcggc gctcgatctc
     1861 ggccagcagc ggcttgttcc agccgagcat gaacatcgcc tcggcggtca cgaacatcgg
     1921 gccgaccagc agcccggtca tgtcgtcgac gaaggccggc ttgcggccct cgtagtagtg
     1981 gccgacgaac tgaagcaccc acccaacgac gaaaaagccc aacccccagg ccagccactc
     2041 gccgatgggg ccgccggcca cttcatgggc cagcgcgacc agccccagcg tgacggcgct
     2101 gatcgcgagg cccagatcga gccggccgc
//
LOCUS       DATMJB010000011         3850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_767757, whole genome shotgun sequence.
ACCESSION   DATMJB010000011 DATMJB010000000
VERSION     DATMJB010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767757"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1282
                     /locus_tag="VNU48_00255"
     CDS             209..1282
                     /locus_tag="VNU48_00255"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HWI09739.1"
                     /translation="MTPPREPLPDPDSPSATPYADLSPDVVLDALDAVGLRGDGRLIQ
                     LNSYENRVFQVFLEDGRVVVTKFYRPQRWSDAQILEEHAFANELAAAEIPVAPVWPLT
                     IATDSRHADRVGLPGPTLAQFATPLGNYRFSVSARLAGRAPELEDAGTLEWIGRFVGR
                     LHAVGAVKPFAFRQTLDPATFGTANRDWLLAHDIIPPDAEPAWRQVTEDALAAVNEAF
                     GHGMQARRLRLHGDCHLGNVLWASDGPHFVDLDDAVMGPATQDLWMLLSGERSAMTRQ
                     LAAVLDGYESFMDFDWRELRLIEPLRTLRIIHHSAWIARRWRDPAFPIAFPWFESPKY
                     WAEQTTRLRQQLDAMAEPPLGGA"
     gene            complement(1314..3194)
                     /gene="htpG"
                     /locus_tag="VNU48_00260"
     CDS             complement(1314..3194)
                     /gene="htpG"
                     /locus_tag="VNU48_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HWI09740.1"
                     /translation="MDKQTLSFQAEVKQILHLVTHSLYSNKEIFLRELISNASDACDK
                     LRFEALNHPALLEDAPNLDVRVAFDADAKTLTITDNGIGLSDDEAVANLGTIAKSGTR
                     EFMSALEGDQKKDAQLIGQFGVGFYSGFIVADRITVESRRAGLPPDQGVRWSSEGSGD
                     FEVETITRAARGTSVTLHLREGEEEFLSGWKLKAIIGKYSDHISLPILMQKEEWDAEA
                     AKQVTKDEWAPVNKAAALWTRSKNDITPEQYAEFYKQLSYDSEPPLAYTHNRVEGRSE
                     YTQLLYVPAKAPFDLWNRDKRGGVKLYVKRVFIMDDAEALMPVYLRFVKGVIDSADLP
                     LNVSRELLQESRDVKAIREGSTKRVLGMLEVLADSEDEAERAKYAAFWKDFGVVLKEG
                     VGEDHANQERLTKLLRFASTHADEGVSFTAYVGRMKEGQEAIYYVTADSLAAAKSSPQ
                     LEIFRKKGIEVLLLTDRVDEWMLSHLYEFDGKPLQSVAKGSVDLGKLQDEAEKKQAEE
                     TATAFKPVLDRLKEALKERAKDVRVTTRLVDSPACLVVEEGDMSGHLARLLKQAGQAA
                     PKSLPTLEVNAEHALVKRLDASAHFDDLANILFDQALLAEGGQLEDPAAYVRRVNALL
                     VA"
     gene            complement(3311..3658)
                     /locus_tag="VNU48_00265"
     CDS             complement(3311..3658)
                     /locus_tag="VNU48_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09741.1"
                     /translation="MSSTSDKLALVGRTIRWRFDDGPVAGQTFEHTFDSDGSVVWRSV
                     DGKDPTKLNHEKFGAVAPISDDVVVVSYAAASGYTLTVALNLAQGRMVGFASGHDTWA
                     QQKGSFELLPEPA"
     gene            complement(3655..>3850)
                     /locus_tag="VNU48_00270"
     CDS             complement(3655..>3850)
                     /locus_tag="VNU48_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWI09742.1"
                     /translation="LAKQGEGFAIDRIALTMQASVPGIDDARFQELAAAAKRDCPLSK
                     ALASVPEITLQATLRSETGR"
BASE COUNT          534 a         1391 c         1251 g          674 t
ORIGIN      
        1 ggcttcagca acagcgagtt cgccccgctg ccgcgcgcca agccgctgcg tccgtcggcg
       61 cgacgtgccg agccgacgag cttcggcgaa gtcgacgtcg atgtcgacat cgacgcgccg
      121 ggcggcggtg tcgtcgtcga cggcccgaaa ttctcgagcg gctcgcggcg ctgaagagtc
      181 ggcccacggc ggcgcgtacg atgcgcgcat gacacccccg cgcgagcccc tgcccgaccc
      241 ggactcgcct tccgccacac cctacgccga cctgagcccc gacgtcgtcc tcgacgcgct
      301 cgacgccgtc ggtctgcgcg gcgatggccg gctgatccag ctcaactcct acgagaaccg
      361 ggtcttccag gtcttcctcg aagacggccg cgtcgtcgtc accaagttct accggccgca
      421 gcgctggagc gacgcacaga tcctcgagga gcacgcgttc gcgaacgagc tggcggcggc
      481 cgagatcccg gtcgcgccgg tgtggccgtt gacgatcgcc accgattcac gccacgccga
      541 ccgcgtcggc ctgcccggcc cgacgctcgc gcagttcgcc acgccgctcg gcaactaccg
      601 cttcagcgtc agcgcgcgcc tcgccggccg cgcgccggag ctcgaagatg cgggcacgct
      661 ggaatggatc ggccgcttcg tcggccgtct gcatgcggtc ggcgccgtca agcccttcgc
      721 gtttcggcaa accctcgatc cggccacctt cggcaccgcc aaccgcgact ggctgctcgc
      781 gcacgacatc atcccgcccg atgccgagcc ggcctggcgc caagtcaccg aagacgcgct
      841 ggccgcggta aacgaagcct tcggtcatgg catgcaggcg cgccgcctgc gcctgcatgg
      901 cgactgccac ctgggcaacg tgctgtgggc gtcggacggc ccgcatttcg tcgatctcga
      961 cgatgccgtg atgggcccgg cgacccagga cctgtggatg ctgctgtcag gcgagcgcag
     1021 cgcgatgacg cgccagctcg ccgcggtgct cgacggctac gagagcttca tggacttcga
     1081 ctggcgcgag ctgcgcctga tcgagccgct gcgcaccttg cgcatcatcc accacagcgc
     1141 ctggatcgcg cggcgctggc gcgacccggc gttcccgatc gcgtttccgt ggttcgagag
     1201 cccgaagtac tgggccgagc agaccacgcg cttgcgccag cagctcgacg caatggccga
     1261 gccgccgctg ggcggcgcgt agcgtctgcc gccggcgagc accggcgaat cgatcaggcc
     1321 accagcagcg cgttgacccg cctcacgtag gcggccgggt cctcgagctg cccgccctcg
     1381 gccagcagcg cctggtcgaa caggatgttg gccaggtcgt cgaaatgcgc gctcgcatcc
     1441 aggcgcttga ccagcgcatg ctcggcgttc acttccagtg tcggcagcga cttcggcgcg
     1501 gcctgcccgg cctgcttcag cagccgcgcg aggtggccac tcatgtcgcc ttcctcgacc
     1561 accaggcagg cgggcgaatc gaccaggcgg gtcgtcacgc gcacgtcctt ggcgcgctcc
     1621 ttcagcgcct ccttcaggcg gtcgagcacc ggcttgaacg cggtcgcggt ctcctcggcc
     1681 tgcttcttct cggcctcgtc ctgcagcttg ccgaggtcga ccgagccctt cgcgacgctc
     1741 tgcagcggct tgccgtcgaa ctcgtatagg tggctgagca tccactcgtc gacccggtcg
     1801 gtcagcagca gcacctcaat gcccttcttg cggaagatct cgagctgcgg gctgctcttc
     1861 gccgcggcga ggctgtcggc ggtgacgtag tagatcgcct cctgaccttc cttcatgcgg
     1921 cccacgtagg cggtgaacga cacgccctcg tcggcatgcg tcgaggcgaa gcgcagcagc
     1981 ttggtcagac gctcctggtt cgcgtgatcc tcgccgacgc cttccttcag caccacgccg
     2041 aagtccttcc agaacgcggc gtacttcgcg cgctcggcct cgtcctcgct gtcggccagc
     2101 acctcgagca tgccgagcac gcgcttggtc gagccttcgc ggatcgcctt cacgtcacgg
     2161 ctttcctgca gcagttcgcg gctcacgttc agcggcaggt cggcgctgtc gattacgccc
     2221 ttgacgaagc gcaggtacac cggcatcagc gcctcggcgt cgtccatgat gaagacgcgc
     2281 ttgacgtaga gcttgacgcc gccgcgcttg tcgcggttcc acaggtcgaa cggcgccttc
     2341 gccggcacgt acagcagttg cgtgtactcg ctgcgaccct cgacgcggtt gtgcgtgtag
     2401 gccagcggcg gctcgctgtc gtagctgagc tgcttgtaga actctgcgta ctgttcgggg
     2461 gtgatgtcgt tcttgctgcg cgtccacagc gccgcggcct tgttgaccgg cgcccactcg
     2521 tccttggtca cctgcttggc ggcctcggcg tcccactctt ccttctgcat caggatcggc
     2581 agcgagatgt ggtcggagta cttgccgatg atggccttga gcttccagcc actcaggaac
     2641 tcttcctcgc cctcgcgcag gtgcagcgtg acgctggtgc cgcgcgccgc gcgcgtgatc
     2701 gtctcgacct cgaagtcgcc gctgccctcg gagctccagc gcacgccctg atcgggtggc
     2761 aggccggcgc gccgcgactc gacggtgatg cggtcggcga cgatgaagcc gctgtagaag
     2821 cccacaccga actggccgat cagctgcgcg tccttcttct ggtcgccctc cagcgccgac
     2881 atgaactcgc gcgtgccgct cttcgcgatc gtgcccaggt tcgcgaccgc ctcgtcgtcc
     2941 gacaggccga tgccgttgtc ggtgatcgtc agcgtcttcg cgtccgcatc gaacgcgacc
     3001 cgcacgtcca ggttcggcgc gtcctcgagc aaggccgggt ggttcagcgc ctcgaagcgc
     3061 agcttgtcgc acgcgtccga cgcgttcgag atcagctcgc gcaggaagat ctccttgttc
     3121 gagtagagcg agtgcgtgac gaggtgcagg atctgcttga cctcggcctg gaaggaaagc
     3181 gtctgtttgt ccatgatctt ttcggcaagt gctgccggac gcggccccgt gccgcccgga
     3241 aaggcggcat tttgggccac gcgcgctcga attcaagaag tccggccgcg cgctgcgcgc
     3301 cggcggccgg tcaggccggc tccggcaaca gctcgaagct gcccttttgc tgcgcccagg
     3361 tgtcgtgccc ggatgcgaag ccgaccatcc tgccctgcgc gaggttcagc gcgacggtca
     3421 gcgtgtagcc gctggccgcg gcgtacgaca ccacgaccac gtcgtcgctg atcggcgcga
     3481 ccgccccgaa cttctcgtgg ttcagcttgg tcgggtcctt gccgtcgacg ctgcgccaga
     3541 cgacgctgcc gtcgctgtcg aaggtgtgct cgaaggtctg gcccgcgacc ggcccgtcgt
     3601 cgaagcgcca gcgaatcgtc cggccgacca gcgccagttt gtcggaagtg ctgctcatcg
     3661 tcccgtctcc gaacgcagcg tcgcctgcaa cgtgatctcg ggcacgctcg cgagcgcctt
     3721 cgacagcggg cagtcgcgct tcgcggcggc cgcgagttcc tggaacctcg cgtcgtcgat
     3781 gccgggcacg ctcgcctgca tcgtcagcgc gatgcggtcg atcgcgaagc cctcgccttg
     3841 tttcgccagg
//
LOCUS       DATMJB010000012         3529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_175308, whole genome shotgun sequence.
ACCESSION   DATMJB010000012 DATMJB010000000
VERSION     DATMJB010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175308"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VNU48_00275"
     CDS             complement(<1..226)
                     /locus_tag="VNU48_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWI09743.1"
                     /translation="MNSQDAKRVLETALICASQPLPLRDMRVLFADEIGPDTLRSLLD
                     ELTREWDGRGVELVALATGWRLQSRPEMRVY"
     gene            complement(524..1528)
                     /locus_tag="VNU48_00280"
     CDS             complement(524..1528)
                     /locus_tag="VNU48_00280"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWI09744.1"
                     /translation="MVQPPAHRPADPADAAEGGADAEPDAPWATQPEQRAALVPSALH
                     GQRLDKAVVAMAPEFSRTHLQGLIERGHVTVDAVPLRVPSRKVLAGQAIAIDLVPTEE
                     SRAFRPEALSIAIVFEDAHLIVIDKAAGMVVHPAAGNWSGTLLNALLAHHRGAASLPR
                     AGIVHRLDKDTSGLMVVGKTLPALTTLTRAIAAREVHREYLALVHGEVHPASFSVDAP
                     IGRDPQSRVRMAVLASGRPARTDVQAVAVAAGYSALRCMLHTGRTHQIRVHLAARGHP
                     LVADAVYGGKPALGMTRQSLHATRLGFVHPASGAAVSFEASPPPDLASAWRNVVQGCA
                     "
     gene            1586..2386
                     /locus_tag="VNU48_00285"
     CDS             1586..2386
                     /locus_tag="VNU48_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HWI09745.1"
                     /translation="MIQLNALRRPRAFALAMLVAVLAGCGTNPKDPESQWSAERLYAE
                     AKEEASQGNYERAGKLYERLEGRASGTALAQQAQIERAYMLYKSADKAQALSTLERFI
                     KLHPTSAAIDYALYLQGLINFNDNLGLLGAIAQQDLSERDQQAARDSYQSFKQLVDQY
                     PQSLYADDARVRMNYIVNALAAYEVHVARYYFRRGAYVAAANRAQMVVSDFQQSPSAH
                     EALYILSESYDKLGLTELRDDANRVMQKNFPTSLDGLGVAKKRWWQLW"
     gene            complement(2394..>3529)
                     /locus_tag="VNU48_00290"
     CDS             complement(2394..>3529)
                     /locus_tag="VNU48_00290"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HWI09746.1"
                     /translation="CDRAARELRIAAAGRLRDLRGTLKLRWNERSGQDDFTSALDAVR
                     QTCEAAAAGLKVVVEMSPDFAKLGERAAQFAALAQRFAEPAADGNVRWIDVSPHQTRL
                     VESPLDIRDALREQMAAAPKTWVFTSATLGDDERLSWFTESAGLTEAITLRLGSPFDY
                     AAHARLYVPRGFPKPNEPEHAASVAVLAARCARALGGRTFVLTTTLRALQAIGERLRA
                     EFDAQGDALQVLVQGQVPKRQLMQQFLAQPRSVLLGSQSFWEGIDVPGDALQCVLIDK
                     LPFPPPNDPLVEARVKRLESEGRNPFADYFVAEAAVSLKQGAGRLIRSETDRGLLVVC
                     DPRMAGMNYGRRLREALPPMTMVATEAEALDWLATLAGAALPF"
BASE COUNT          524 a         1263 c         1223 g          519 t
ORIGIN      
        1 ggtagacacg catctcgggt cggctttgca ggcgccagcc ggtggccagt gccaccagtt
       61 cgacgccgcg gccatcccac tcgcgcgtca gctcgtcgag cagcgaacgc aaggtgtcgg
      121 ggccgatctc gtcggcgaac agaacgcgca tgtcacgcaa gggcagcggc tggcttgcgc
      181 agatcagggc ggtctcgagg acgcgcttcg catcctggct attcatggac ttcgttcaca
      241 tcctcggccg gatcgggccg gctggtactc gacagacatt cagtgccggt gctgggcttc
      301 gcttgcgctg cgcgcccggc ttgtgggcac gggcactggc gttcgcggcg ggcaggtggg
      361 tgacgcttct cgcgtcgctc cggtatgggg caccttcggt gcgcggctca cttccagatg
      421 ggcttcgtcg ttgaccgggg gcaccgggcg gtgccgccgc gaggatcggc gtcgggaagg
      481 attggccgca ttgtagccgc gaccgccgcg tgtgcgtgcg ccgtcaagcg caaccctgca
      541 cgacgttgcg ccaggccgac gccaggtcgg gcggcggcga tgcctcgaac gacaccgctg
      601 cgcccgacgc gggatggacg aatccgagcc gcgttgcatg cagcgactgg cgcgtcatgc
      661 cgagtgccgg cttgccgccg tagaccgcat cggccaccag cgggtgaccg cgcgcggcga
      721 ggtgcacgcg gatctggtgc gtgcggccgg tgtgcagcat gcagcgcagc gcgctgtagc
      781 cggccgcgac cgcgacggcc tgcacgtcgg tgcgcgccgg ccggcccgat gccagcaccg
      841 ccatgcgtac ccgcgattgc ggatcgcggc cgatcggcgc gtcgacgctg aagctggccg
      901 ggtgcacctc gccgtgcacc agcgcgaggt attcgcggtg cacctcgcgc gccgcgatcg
      961 cgcgcgtcag cgtcgtcagc gcgggcagcg tcttgccgac caccatcagg cccgaggtgt
     1021 ccttgtcgag ccggtgcacg atgccggcac gcggcaggct tgcagcgccg cggtgatgag
     1081 ccaacagtgc gttcaacagc gtgcccgacc agttgcccgc cgcgggatgc acgaccatgc
     1141 cggcggcctt gtcgatcacg atcaggtgcg cgtcctcgaa cacgatcgcg atcgacaagg
     1201 cctccggccg gaacgcccgg ctctcctccg tgggcaccag gtcgatcgcg atcgcctggc
     1261 cggccagcac cttgcgcgac ggcacgcgca gcggcacggc atccacggtg acatggccgc
     1321 gctcgatcag gccctgcagg tgggtgcgcg agaactccgg tgccatcgcg acgaccgcct
     1381 tgtccaggcg ctgtccgtgc agcgccgatg gcaccagggc cgcgcgctgt tccggctgcg
     1441 tcgcccaggg cgcgtcgggc tcggcgtcgg caccgccctc ggcggcatcg gccggatcgg
     1501 cgggcctgtg cgccgggggc tgaaccatat aatcgcgact cgttttctga caggacggcg
     1561 ccgctctcgc gaacgcccca ggccgatgat tcaattgaat gcgctgcgac ggccccgtgc
     1621 cttcgcactg gccatgctgg tggccgtgct cgcgggctgc ggtacgaacc ccaaggaccc
     1681 cgagtcacag tggagcgcgg agcgattgta tgcagaggcc aaggaagagg cctcgcaagg
     1741 caactacgaa cgcgccggca agctttacga gcggctcgag ggccgcgctt ccggcaccgc
     1801 gctggcgcag caggcgcaga tcgagcgtgc ctacatgctc tacaagagcg ccgacaaggc
     1861 gcaggcgctg tccacgctcg agcgcttcat caagctgcac ccgaccagcg ccgcgatcga
     1921 ctacgcgctg tacctgcagg gcctgatcaa tttcaacgac aacctcggcc tgctcggcgc
     1981 gatcgcgcag caggacctct ccgaacgcga ccagcaggcc gcgcgcgatt cataccagtc
     2041 gttcaagcag ctggtcgacc agtacccgca atcgctgtac gccgacgatg cgcgcgtgcg
     2101 catgaactac atcgtcaatg cgctggcggc ctacgaggtg cacgtggcgc gctactactt
     2161 ccggcgcggc gcgtatgtcg cggcggcgaa ccgcgcgcag atggtggtgt cggacttcca
     2221 gcagtcgccc tcggcgcatg aggcgctgta catcctgtcg gagagctatg acaagctcgg
     2281 cctgaccgaa ctgcgcgacg acgccaatcg cgtgatgcag aagaacttcc cgacgtcgct
     2341 cgacggcctc ggcgtggcca agaagcgctg gtggcagctt tggtgagtcg cttctagaac
     2401 ggcagcgcgg cgccggccag cgtcgccagc cagtccagcg cctcggcctc ggtggccacc
     2461 atcgtcatcg gcggcagcgc ctcgcgcagc cgcctgccgt agttcatgcc cgccatgcgc
     2521 gggtcgcaca ccaccagcag gccgcgatcg gtctcgctgc ggatcagccg cccggcgccc
     2581 tgcttcagcg acaccgccgc ctcggccacg aaatagtccg cgaacgggtt gcggccctcg
     2641 ctttcgaggc gcttgacccg ggcctcgacc agtgggtcgt tcggcggcgg gaacggcagc
     2701 ttgtcgatca gcacgcactg cagcgcgtcg ccgggcacgt cgatgccctc ccagaaactc
     2761 tgcgagccga gcagcaccga gcgcggctgc gccaggaact gctgcatcag ctggcgcttc
     2821 ggcacctgac cctgcaccag cacctgcagc gcgtcgccct gcgcatcgaa ctcggcccgc
     2881 agccgttcgc cgatcgcctg cagcgcgcgc aaggtcgtcg tcagcacgaa ggtgcgcccg
     2941 ccgagcgcgc gcgcacagcg cgccgcgagc accgccaccg acgcggcatg ctccggctcg
     3001 ttcggtttcg gaaagccgcg cggcacgtac aggcgcgcgt gcgccgcgta gtcgaacggg
     3061 ctgcccaggc gcagcgtgat cgcctcggtc agcccggccg attcggtgaa ccacgacagg
     3121 cgttcgtcgt cgccgagcgt ggccgaggtg aagacccagg tcttgggcgc cgccgccatc
     3181 tgctcgcgca gcgcgtcgcg gatgtccagc ggcgactcga ccagccgcgt ctggtgcggc
     3241 gagacgtcga tccagcgcac gttgccgtcc gcagccggct cggcgaagcg ctgcgcgagc
     3301 gcggcgaact gcgccgcccg ctcgccgagc ttggcaaagt ccggcgacat ctcgaccacg
     3361 accttcagcc ccgccgcggc cgcctcgcag gtctggcgca cggcgtcgag cgcgctggtg
     3421 aaatcgtcct gtccgctgcg ttcgttccag cgcagcttca gcgtgccgcg cagatcgcgc
     3481 aggcgcccgg cggccgcgat gcgcagctcg cgcgccgcgc gatcgcagg
//
LOCUS       DATMJB010000013         2924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_471303, whole genome shotgun sequence.
ACCESSION   DATMJB010000013 DATMJB010000000
VERSION     DATMJB010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471303"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1988
                     /locus_tag="VNU48_00295"
     CDS             <1..1988
                     /locus_tag="VNU48_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWI09747.1"
                     /translation="RLPGVTLQGRPGRGGNIRLRGLGQGYTQILLDGQRIPPGFSLDS
                     LTPDQVERIEILRAPTAETGARAIAGTINIITREGFNKHVNDLHLTAGLENGRPQPHV
                     AWTGNEPVGPFLVNTSLSLFGVDHDSDSTTTTLDRSLSDGSVSLDQRDVGTVREKRHG
                     VHLSGRLQWRAGSGLDSVTLLPLLLHGLGTTRRSGTLTQTVGAQPPAYGQSATDGDVS
                     YTLLRLNGHWNHRVGAGGRLEGNLGLGQTRVLSASLRREYSAGALSRTLDDHGDTLDR
                     NLLLGGKFVRVLAGDHSLVAGVEGEANRREEQRRTDDPALLANFGDNVQASALRLAGY
                     AQDEWNATPQWALHAGLRWEGITTRGASGENGSTGQVNRSGVWTPLLHAVWKPEPESR
                     DQLRLSLTRSYRSPTLQSLIGRPSINNRNPAPGSNTPTQPDRAGNPALRPELATGIDI
                     AVERYLTGSGLLSVNVFQRQIRNYMRSQTMLEAVSWSPGQPRYVSRPQNIGAAMTQGV
                     ELEAKFRLSELIAEAPKIDLRTNASVFRSRVKAVPGPDNRLDQQPGYTANLGADYRVP
                     GWPLTIGGNLNWTPAYDTRVSEIQTAYQGRKLVIDAYGLWVFNPTWQLRLTASNLNPC
                     DYLTAGSVDDLGAGLRETSTTIAPTYLNLQLRLEIKL"
     gene            2227..>2924
                     /locus_tag="VNU48_00300"
     CDS             2227..>2924
                     /locus_tag="VNU48_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI09748.1"
                     /translation="MPLFHPSKPRRAALAVLSIAATIGASPLALAQGKGEIRIAHVYS
                     KSGPLEAYGKQTQTGLAMGLDYATGGTMMVAGKKLVLIEKDDQGKPDLGKSLLATAYG
                     DDKADIAVGPSSSGVALALLPVAEEYKKILLVEPAVADSITGDKWNRYIFRTGRNSSQ
                     DAISNAVASDFDGVSIGTLAQDYAFGRDGVKAFKNALKKAKIVHEEYLPPNTTDFTAG
                     VQRLVDALKDKPGR"
BASE COUNT          516 a         1048 c          985 g          375 t
ORIGIN      
        1 agcgcctgcc cggggtgacg ctgcagggcc ggcccgggcg tggcggcaac atccggctgc
       61 gcggcctggg acagggctac acgcagatcc tgctcgacgg gcagcgcatc ccgccggggt
      121 tctcgctcga ctcgctgaca ccggaccagg tcgagcgcat cgagatcctg cgcgcgccga
      181 cggcagagac cggcgcacgc gcgatcgccg gcacgatcaa catcatcacg cgcgagggct
      241 tcaacaagca cgtcaacgac ctgcacctca ctgcggggct cgagaacggg cggccgcagc
      301 cgcatgtcgc gtggacgggc aacgagcccg tcgggccgtt cctcgtcaac acctcgctgt
      361 cgctgttcgg cgtcgaccac gacagcgaca gcacgacgac gacgctcgat cgcagcctga
      421 gcgacggcag cgtgagcctg gaccagcgcg acgtcggcac ggtgcgcgag aagcgccacg
      481 gcgtgcacct cagcggccgg ctgcaatggc gcgccgggtc ggggctggac tcggtgacct
      541 tgctgccgct gctgctgcac ggcctcggca ccacgcggcg cagcggcacg ctgacccaga
      601 ccgtcggcgc gcagccgccc gcgtacgggc agagcgcgac cgatggcgac gtgagctaca
      661 cgctgctgcg gctgaacggc cactggaatc atcgcgtcgg ggccgggggg cgcctcgaag
      721 gcaacctcgg cctcggccag acccgcgtgc tcagcgcgag cctgcgccgc gagtacagcg
      781 ccggcgcgct gagccgcacg ctcgatgatc atggcgacac gctggaccgc aacctgctgc
      841 tcggcggcaa gttcgtgcgc gtgctggccg gcgaccacag cctcgtcgcc ggcgtcgagg
      901 gcgaggcgaa ccgccgcgag gaacagcgcc ggaccgacga cccggcgctg ctcgccaact
      961 tcggcgacaa cgtgcaggcg tcggcgctgc gcctggccgg ctacgcgcag gacgaatgga
     1021 acgcgacgcc gcagtgggcg ctgcatgccg ggctgcgctg ggaaggcatc acgacgcgcg
     1081 gcgcgagcgg cgagaacggt tcgaccgggc aggtcaaccg cagcggcgtg tggacgccgc
     1141 tgctgcacgc cgtctggaag cccgagcccg agagccgtga ccagcttcgg ctcagcctga
     1201 cgcgcagcta ccgatcgccg acgctgcaaa gcctgatcgg ccggccgagc atcaacaacc
     1261 gcaatccggc gccgggcagc aacacgccga cccagcccga ccgcgccggc aacccggcgc
     1321 tgcggcccga actcgccacc ggcatcgaca tcgcggtcga gcgctacctg accggcagcg
     1381 ggctgctgag cgtcaacgtg ttccagcgcc agatccgcaa ctacatgcgc agccagacga
     1441 tgctcgaggc cgtgtcctgg tcgccgggcc agccgcgcta tgtctcgcgg ccgcagaaca
     1501 tcggcgcggc gatgacgcag ggcgtcgaac tcgaggccaa gttccgcctc agcgagctca
     1561 tcgccgaggc gccgaagatc gatctgcgca cgaacgccag cgtgttccgc tcgcgcgtga
     1621 aggcggtgcc cggccccgac aaccggctcg accagcaacc gggctacacc gccaacctcg
     1681 gcgccgacta ccgcgtgccg ggctggccgc tgacgatcgg cggcaacctg aactggacac
     1741 cggcctacga cacccgcgtc agcgagatcc agaccgccta ccagggccgc aagctcgtga
     1801 tcgacgcgta cgggctgtgg gtcttcaacc cgacatggca acttcgcctc accgccagca
     1861 acctgaaccc gtgcgactac ctgaccgccg gcagcgtcga cgaccttggc gcgggcttgc
     1921 gggagacctc gacgaccatc gcgccgacct acctgaatct gcagctgcgc ctggagatca
     1981 agctctgaga cgcacctccg gacagtgact gtatttcagg cagttcacgc agaatatgca
     2041 tgaactccgg tcagccgtgg cccccgttgc gtggccgatc cggctctatc accgggcgga
     2101 cgggcggatt actcagggtt tgcactaagt cggtgcgccg tggcacaacg attgcactcc
     2161 caggtcatcc ggcgcctgcg cccggccatt ccgatccagc aacgagcccg ttcaggagac
     2221 atccccatgc cgctgttcca tccctcgaag ccgcgccgcg cggcgctcgc cgtcctgtcg
     2281 atcgccgcca cgatcggtgc ctcgccgctg gcgctggcgc agggcaaggg cgagatcagg
     2341 atcgctcatg tctacagcaa gtcgggcccg ctcgaggcct acggcaagca gactcagacc
     2401 ggcctcgcga tgggcctgga ctacgcgacc ggcgggacga tgatggtcgc cggcaagaag
     2461 ctggtgctga tcgagaaaga cgaccagggc aagcccgatc tgggcaagag cctgctggcc
     2521 accgcctatg gcgacgacaa ggccgacatc gcggtcgggc cgagctcgtc gggcgtggct
     2581 ctggcgctgc tgccggtggc cgaggagtac aagaagatcc tgctggtcga gcccgcggtg
     2641 gccgattcga tcaccggcga caagtggaac aggtacatct tccgcaccgg ccgcaacagc
     2701 tcgcaggacg cgatcagcaa cgcggtcgcg tccgacttcg acggcgtctc gatcggcacg
     2761 ctggcgcagg actacgcgtt cgggcgcgac ggcgtgaagg ccttcaagaa tgcgctgaag
     2821 aaggccaaga tcgtccacga ggaatacctg ccgccgaaca ccaccgactt cactgccggc
     2881 gtccaacgcc tcgtcgacgc gctgaaggac aagccgggcc gcaa
//
LOCUS       DATMJB010000014         6308 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_115566, whole genome shotgun sequence.
ACCESSION   DATMJB010000014 DATMJB010000000
VERSION     DATMJB010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115566"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VNU48_00305"
     CDS             complement(<1..469)
                     /locus_tag="VNU48_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09749.1"
                     /translation="MAQAAVGIGRGARLGPERVDERVVFAREQRVIEGDPDAPVVGKR
                     RRALAGPLRQDRVAGVEVDGRVAPIRWFSLEGSVTWQNPEYANLQNTSGADPSAVNGK
                     QIIREAKIFGNLRPSFDFDLGEQKLSIFGRFEWVGRRYVDLFNRTAMPGYQATG"
     gene            383..1915
                     /locus_tag="VNU48_00310"
     CDS             383..1915
                     /locus_tag="VNU48_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HWI09750.1"
                     /translation="MFAREHNAFVDAFRAQARATPDADSGLRHPLRPDAPIRYREVTA
                     DELFEVGRLVVAAEIAKIHTIEWTPQLLYDEPLYLAMNSNWNGLFDGREIAAAALEKI
                     VRRLGESDDAKKATQLYAAFASGPGIFGLGNHRYADGTTTARNDPSKNDLWSVANPDD
                     VNGGTNHFGSPFNFPEEFVTAYRLHALVPDLIEYREWDRDPNLIRAKVPVVETFRAKA
                     TPALVDAGAANWALSMGRQRLGLLTLQNTPAFLQNLAIPRLQSATGKIDVAALDLIRD
                     REHGVPRFNEFRRQYGLRQLTRFDDFADQRLPAGAPERAQQQRVVALLRQIYGQHRCD
                     ASKIITAAQRNPDGTRIDDCLGLPDGSLVDNIEDVDTVVGWLAEYPRPHGYAISETQF
                     QVFILNASRRLFSDRFFTSSFRPEFYSALGVRWVEDNGPLGKLFEPQPSNGHRVEVAP
                     LKRVLLRTMPELAPELAHVVNSFDPWGRDRGDYYTLRWQPRPGAERDPAFAEGDPAFK
                     AP"
     gene            complement(1902..3428)
                     /gene="malQ"
                     /locus_tag="VNU48_00315"
     CDS             complement(1902..3428)
                     /gene="malQ"
                     /locus_tag="VNU48_00315"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWI09751.1"
                     /translation="MDLNQRSAGVLLHLSSLPGPHGIGDLGPDAFHFVDWLASAGQRV
                     WQVLPTTPIGPGDSPYQGVSAFAGSPLMVALEPLVEQGWLAAPALPPGGFDAARVDYG
                     RVAPWRLAQLRAAAAGFFDRAAPAEREAFAAWCAGQTHWLADYALFMALENAHAGASW
                     WHWPAALREREANALADARRTLAAELRFWQFVQWCFDTQWAALRAHANARGVSIMGDL
                     PIFVAHHSADCWARPDLYELDAEFQPAVVSGVPPDAFSADGQRWGNPLYRWDRMAAEG
                     YAWWVARMRRELAHADAFRIDHFRGFAAHWEVPATSPTAIAGRWVPGPGRALFDALAR
                     ALGPLPIIAEDLGVITPDVTALREACGFPGMRILQFAFGDDARNPYLPHRHTPASVIY
                     TGTHDNDTTLGWWQRLGQAERAFAAIYLGVDRDATGSEVCAALIRAAYTSVANLAVVP
                     MQDVLRLDGTHRMNLPGAATGQWAWRFDWGGVGPAPAHALARLAGATGRGAFAAVTAP
                     "
     gene            3576..6257
                     /locus_tag="VNU48_00320"
     CDS             3576..6257
                     /locus_tag="VNU48_00320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HWI09752.1"
                     /translation="MSVRHLEQLLNPRSVVVIGASDRTGSVGATVWRNLRAGQFTGTV
                     HAVNPRHTMLDEQPVFARVADLPLAPDLALICTPPDSVAGLIDELGQRATRAAVVMTA
                     GLGPGRMQALRDAARPHLLRVLGPNCLGLLTPHLGLNASFAHTDALVGDVAFVSQSGA
                     LVTAVLDWAKGRRIGFSHMVSLGEHADVDFGDLLDHLASDARTRSILLYIESIEAPRK
                     FMSAARAAARNKPVIVVKAGRAGNGVKAAMSHTGALAGSDVVFDAAIRRAGMLRVDTL
                     QDLFMAAETLARFRGTDNRCLTLMTNGGGAGVMAADAAALAGIPLAELGDDLRARLDA
                     ALPPTWSGANPIDIIGDAPVERYTATLQALLAERSSGTVLFLHAPTAIVRSEDIARAC
                     APLVQPAAGRVMSSWLGDAAVADARRIFEDAGIADYETPEEAIRAFGMVRTYRRNQAL
                     LSEAPTASELGTPDLASARAIIRAALADGRELLDELEAKNVLKACGVPVVPTLAVGAS
                     ADAAARGAREIGYPVALKILSPDITHKSDAGGVQLNLHDEAELRQAVAAMLMRVGTAR
                     PEARLDGFTVQAMVRRPMAQELIVGASVDPTFGPVLLFGQGGTAVEVLGDRAIALPPL
                     NRVLARDLISHTRVAKLLAGYRDHPPARLDAIADVLIALSQMLADLPELAELDINPLL
                     ADDQGVIALDARLRVSRSAGAGTECFAIQPYPPELAERVAWQGRDLLLRPIRPEDEAI
                     HRSFVERLSPEDLRLRFFSVRRELPRSELARLVQIDYSREMAFIALAPDAGGALETLG
                     VVRAVADPDNREAEFAVIVRSDLKGHGLGDLLMAKILRYLRGRGTARVVGDVLHENMP
                     MRDLALRHGFRVDEASRDDGALHYVLDLQPGTAEVTD"
BASE COUNT          908 a         2318 c         2220 g          862 t
ORIGIN      
        1 cgccggtcgc ctggtagccc ggcatcgcag tgcggttgaa cagatcgaca tagcgccggc
       61 cgacccattc gaagcgcccg aagatcgaga gcttctgttc gcccaggtcg aagtcgaagc
      121 tcggccgcag attaccgaag atcttcgcct cgcggatgat ctgcttgccg ttcaccgcgc
      181 tcggatcggc gcccgaggtg ttctggagat tggcatattc cgggttctgc caggtcacgg
      241 agccctcgag cgagaaccag cggatcggcg cgacccggcc atccacttcg acgccggcga
      301 cccgatcctg ccgcagtggg ccggccaggg cgcggcggcg tttcccgaca actggagcat
      361 cggggtcgcc ttctatcaca cgctgttcgc gcgcgaacac aacgcgttcg tcgacgcgtt
      421 ccgggcccag gcgcgcgcca cgcccgatgc cgacagcggc ctgcgccacc ctttgcggcc
      481 cgacgcgccg atccgctacc gcgaggtgac cgccgacgag ttgttcgagg tcggccggct
      541 cgtcgtcgcg gccgagatcg cgaagatcca caccatcgag tggacgcctc agttgctcta
      601 cgacgagccg ctctacctgg ccatgaactc gaactggaac ggcctgttcg acggccgcga
      661 gatcgccgcc gccgcgctcg agaagatcgt gcggcgcctg ggcgaatcgg acgacgcgaa
      721 gaaggcgacg caactctatg cggcgttcgc gtccggcccc ggcatcttcg gcctgggcaa
      781 ccaccgctat gccgacggca cgacgaccgc gcgcaacgac ccgtcgaaga acgacttgtg
      841 gagcgtcgcg aacccggacg acgtgaacgg cggcaccaac cacttcggct cgccgttcaa
      901 cttcccggaa gagttcgtca ccgcgtaccg gctgcatgcg ctggtgcccg acctgatcga
      961 gtaccgcgag tgggaccgcg acccgaacct gatccgcgcc aaggtgccgg tggtcgagac
     1021 cttccgcgcc aaggccacgc cggcgctggt cgacgccggt gcggcgaact gggcgctgtc
     1081 gatgggccgc cagcgcctcg gtctgctgac gctgcagaac acgccggcgt tcctgcagaa
     1141 cctggcgatc ccgcggctgc agtcggcgac cggcaagatc gatgtcgcgg cgctcgacct
     1201 gatccgcgac cgcgagcacg gcgtgccgcg cttcaacgag ttccggcgtc agtacgggct
     1261 gcgccagctg acgcgcttcg acgacttcgc cgatcagcgg ctgccggccg gcgcgcccga
     1321 gcgggcgcag cagcagcgcg tggtcgcgct gctgcgccag atctacggcc agcaccgctg
     1381 cgacgcgagc aagatcatca ccgccgcgca gcgcaacccc gacggcacac gcatcgacga
     1441 ctgcctgggc ctgcccgacg gcagcctcgt cgacaacatc gaagacgtcg acaccgtcgt
     1501 cggctggctc gccgagtacc cgcgcccgca cggttatgcg atctcggaga cgcagtttca
     1561 ggtgttcatc ctgaacgcgt cgcggcgcct gttcagcgac cgcttcttca cgtccagctt
     1621 ccggcccgag ttctactcgg cgctcggcgt gcgctgggtc gaggacaacg ggccgctcgg
     1681 caagctgttc gagccgcagc cgtcgaacgg ccaccgggtc gaggtcgcgc cgctcaagcg
     1741 ggtgctgctg cgcacgatgc ccgagctcgc ccccgaactc gcgcacgtgg tcaacagctt
     1801 cgacccctgg ggccgcgacc gcggcgacta ttacacgctg cgctggcagc cgcgccccgg
     1861 agccgaacgc gaccccgcat tcgctgaagg cgacccggcg ttcaaggcgc cgtgacggcg
     1921 gcgaacgcgc cgcggccggt cgcgcccgcg aggcgcgcga gcgcgtgcgc cggcgccggc
     1981 ccgaccccgc cccaatcgaa gcgccaggcc cactggccgg tcgccgcgcc gggcaggttc
     2041 atgcggtgcg tgccgtccag gcgcagcacg tcctgcatcg gcacgaccgc caggttcgcc
     2101 accgaggtgt aggcggcgcg gatcagcgcc gcgcacacct cgctgccggt cgcatcgcga
     2161 tcgacgccga ggtagatcgc cgcgaacgca cgctcggcct ggccgagccg ctgccaccag
     2221 cccagcgtcg tgtcgttgtc gtgcgtgccg gtgtagatca ctgatgccgg cgtgtgccgg
     2281 tgcggcaggt acgggttgcg cgcgtcgtcg ccgaacgcga actgcaggat gcgcatgccc
     2341 ggaaagccgc aggcctcgcg cagcgcggtc acgtcgggcg tgatcacgcc caggtcctcg
     2401 gcgatgatcg gcagcggccc gagcgcgcgc gcgagcgcgt cgaacagtgc ccggccgggg
     2461 ccgggcaccc agcggccggc gatcgcggtc gggctcgtcg ccggcacttc ccaatgcgcc
     2521 gcgaagccgc ggaagtgatc gatgcggaac gcgtcggcat gcgccagctc gcgccgcatg
     2581 cgcgcgaccc accatgcgta gccctcggcc gccatgcggt cccagcggta cagcggattg
     2641 ccccagcgct ggccatcggc gctgaacgcg tccggcggca cgccggagac gaccgccggc
     2701 tggaactcgg cgtcgagctc gtacaggtcc ggccgcgccc agcagtcggc gctgtgatgg
     2761 gcgacgaaga tcggcaggtc gcccatgatg gacacgccgc gcgcgttcgc gtgcgcgcgc
     2821 aacgcggccc actgggtgtc gaagcaccat tgcacgaact gccagaagcg cagctcggcg
     2881 gcgagcgtgc gccgcgcgtc ggccagcgcg ttcgcctcgc gctcgcgcag cgccgccggc
     2941 cagtgccacc acgacgcgcc ggcgtgcgcg ttctccagcg ccatgaacag cgcgtagtcg
     3001 gcgagccagt gcgtctggcc ggcgcaccac gcggcgaagg cctcgcgctc ggccggcgcg
     3061 gcgcgatcga agaagccggc ggccgcggca cgcagctgcg ccaggcgcca gggcgcgacg
     3121 cgcccgtagt cgacccgcgc cgcgtcgaag ccgccgggcg gcagcgcggg cgcggcgagc
     3181 cagccctgct cgacaagcgg ctcgagcgcc accatcagcg ggctgccggc gaaggccgag
     3241 acgccttgat acggcgaatc cccgggcccg atcggcgtcg tcggcagcac ctgccagacg
     3301 cgctggcccg ccgacgcgag ccagtcgacg aagtggaacg cgtcggggcc gaggtcgccg
     3361 atgccgtgcg ggccgggcag cgagctcagg tgcagcagca cgccggcgct gcgttggttg
     3421 aggtccatgg gccggattgt gccgggctcg cgcgcgccgc cgccgatcag gcctgctcgc
     3481 gctcgctgag ttcggccagc ccgcgcaggc gttgcgccgc gtcaagccgg gggctcgacg
     3541 cggccccgat actgcacgga cccagccgag gtcccatgag cgtccgtcat ctcgagcaac
     3601 ttctgaatcc acgctccgtc gtggtcatcg gtgcgtccga tcgcaccggt agcgtcggtg
     3661 cgacggtctg gcgcaacctg cgagccggtc aattcaccgg gaccgtgcac gcggtgaatc
     3721 cacgccacac catgctcgac gagcagccgg tgttcgcgcg ggtcgccgat ctgccgctgg
     3781 cgccggatct ggcgttgatc tgcacgccgc ccgacagcgt cgcgggcctg atcgacgaac
     3841 tcggccagcg cgccacgcgc gccgcggtcg tcatgacggc cgggctgggc cctgggcgca
     3901 tgcaggcgct gcgcgacgcc gcgcggccgc acctgctgcg cgtgctcggc ccgaattgcc
     3961 tggggctgct gaccccgcac ctcgggctga acgcgagctt cgcgcacacc gatgcactgg
     4021 tcggcgacgt cgcgttcgtg tcgcagtccg gcgccctcgt caccgccgtg ctcgactggg
     4081 ccaagggccg ccggatcggc ttctcgcaca tggtctcgct cggggaacac gccgacgtcg
     4141 atttcggcga cctgctcgac cacctggcca gcgacgcccg cacgcgctcg atcctgctgt
     4201 acatcgaatc gatcgaggcg ccgcgcaagt tcatgtcggc ggcgcgcgcg gcggcgcgca
     4261 acaagccggt gatcgtggtc aaggccggtc gcgccggcaa cggtgtcaag gccgcgatgt
     4321 cgcacacggg cgccctggcc ggctcggacg tggtgttcga cgccgcgatc cgccgtgccg
     4381 gcatgctgcg ggtcgacacc ttgcaggatc tgttcatggc cgcggagacg ctggcgcgct
     4441 ttcgcggcac cgacaaccgc tgcctgacgt tgatgacgaa cggtggcggc gccggcgtga
     4501 tggcggccga tgcggccgcg ctcgcgggca tccctctggc cgaactcggc gacgatctgc
     4561 gcgcccgcct cgacgcggcg ctgcccccga cctggtccgg agcgaacccc atcgacatca
     4621 tcggcgacgc gccggtcgaa cgctacaccg cgacgctgca ggccctgctg gccgaacgca
     4681 gcagcggcac cgtgctgttc ctgcacgcgc cgaccgcgat cgtgcgcagc gaagacattg
     4741 cgcgagcctg cgcaccgctg gtgcaacccg ccgccggccg cgtgatgtcg agctggctcg
     4801 gcgatgccgc ggtggccgac gcgcgccgca tcttcgagga cgcggggatc gccgactacg
     4861 agacccccga agaggcgatc cgcgcgttcg gcatggtgcg gacctaccga cgcaatcagg
     4921 ccctgctgag cgaagcgccg accgcgagcg agctcggcac gcccgacctc gccagcgcgc
     4981 gcgcgatcat ccgcgcggcc ctggccgatg gccgcgagct gctcgacgag ctcgaggcga
     5041 agaacgtgct caaggcctgc ggcgtgccgg tcgtgccgac gctcgcggtc ggcgcgtcgg
     5101 ccgacgcggc ggcgcgcggc gcgcgcgaga tcggctaccc ggtcgcgctg aagatcctgt
     5161 cgcccgacat cacgcacaag tccgacgccg gcggtgtgca gctgaacctg cacgacgagg
     5221 ccgagctgcg ccaggccgtc gcggccatgc tgatgcgcgt cggcacggcg cggcccgaag
     5281 cccgcctcga cggcttcacg gttcaggcaa tggtgcggcg gccgatggcg caggagttga
     5341 tcgtcggggc cagcgtcgac ccgaccttcg ggccggtgct gctgttcgga cagggcggca
     5401 ccgcggtcga ggtgctcggc gaccgggcca tcgcgctgcc gccgctgaac cgcgtgctgg
     5461 cgcgcgacct gatctcgcac acgcgcgtgg ccaagctgct ggccggctac cgcgaccacc
     5521 cgccggcgcg cctcgacgcc atcgccgacg tgctgatcgc gctgtcgcag atgctcgccg
     5581 acttgcccga gctcgccgaa ctcgacatca acccgctgct cgccgacgac cagggcgtga
     5641 tcgcgctcga cgcgcgcctg cgcgtcagcc gctcggcggg cgcgggcacc gagtgcttcg
     5701 cgatccagcc ctacccgccg gagctcgcag agcgcgtcgc gtggcagggg cgcgacctgc
     5761 tgctgcgccc gatccgcccc gaagacgagg cgatccaccg aagcttcgtc gagcggctgt
     5821 cacccgagga tctgcggctg cgcttcttca gcgtgcggcg cgaactgccg cgcagcgagc
     5881 tcgcgcggct ggtgcagatc gactattcgc gcgagatggc cttcatcgcg ctggcaccgg
     5941 atgccggggg cgcgctcgag acgctcggcg tggtgcgcgc ggtggccgac ccggacaacc
     6001 gcgaggccga gttcgcggtc atcgtgcgct ccgacctgaa gggccacggc ctgggcgatc
     6061 tgctgatggc caagatcctg cgttacctgc gcggccgcgg taccgcgcgt gtcgtcggcg
     6121 acgtgctgca cgagaacatg ccgatgcgcg acctggccct gcgccacggc tttcgcgtgg
     6181 acgaggcgag ccgcgatgac ggcgcgctgc actacgtgct cgacctgcaa cccggaactg
     6241 ccgaggtgac ggattgaatg ccgtgccgag gctgtgcgcc ttcctccatg tggctagcaa
     6301 ttgctgat
//
LOCUS       DATMJB010000015         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_885735, whole genome shotgun sequence.
ACCESSION   DATMJB010000015 DATMJB010000000
VERSION     DATMJB010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885735"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /gene="hemW"
                     /locus_tag="VNU48_00325"
     CDS             complement(<1..908)
                     /gene="hemW"
                     /locus_tag="VNU48_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HWI09753.1"
                     /translation="MRGGTLSLAALPPLSLYVHLPWCLKKCPYCDFNSHELQGELAEA
                     RYLDALCADLEAALPFIWGRRVQTVFIGGGTPSLFTPAAIDRLIADIRARLPLEAGCE
                     ITLEANPGTFERDRFRGYRAAGVTRLSVGVQSFDDAKLAALGRVHDARQARAAVEEAR
                     DAFETFNLDLMYALPGQTLAQFEADLAQALAFEPPHLSVYHLTLEPNTVFAKFPPRDL
                     PEDDTAFEMLDVICARTTAAGMERYEVSAFARPGHRCEHNLNYWQFGDYLGIGAGAHS
                     KLSFPHRVVRQTRFREPQKYMAQALAG"
     gene            complement(976..1605)
                     /locus_tag="VNU48_00330"
     CDS             complement(976..1605)
                     /locus_tag="VNU48_00330"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HWI09754.1"
                     /translation="MRLVLASNNAKKLVELQALLAPVGVELVAQGALGVGEADEPFHT
                     FIENGLTKARHAARHTGLAALADDSGLCVEALGGAPGVASAHYAALSPRDGDRETQRA
                     RQDAANNALLLERLHGATHRRARFVSTLVAVRSADDPEPLIAVGRWFGEILSAPRGTG
                     GFGYDPLMFIPALGATVAELDADTKNAHSHRALAAAQMLMLMREAWSLD"
     gene            complement(1608..>2182)
                     /gene="rph"
                     /locus_tag="VNU48_00335"
     CDS             complement(1608..>2182)
                     /gene="rph"
                     /locus_tag="VNU48_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HWI09755.1"
                     /translation="PFLRNSGSGWVTAEYGMLPRSTHTRTDREAARGKQSGRTQEIQR
                     LIGRSLRCVVDLAALGERTISLDCDVIQADGGTRTAAITGAFVAAHDAVTWLQAQGKL
                     SASPIREFVAAVSVGIVQGTPLLDLEYTEDSACDTDMNVVMTGAGGFVEVQGTAEGAA
                     FSRDEMGALLGLADQGIRALIAAQRRALGV"
BASE COUNT          325 a          772 c          780 g          305 t
ORIGIN      
        1 ccggcgagcg cctgggccat gtacttctgc ggttcgcgaa agcgcgtctg gcgcacgacg
       61 cggtgcggga acgacagctt gctgtgcgcg ccggcaccga tgcccagata gtcgccgaac
      121 tgccagtagt tcaggttgtg ctcgcaacgg tggcccgggc gcgcgaaggc cgagacctcg
      181 tagcgctcca tgccggcggc cgtggtgcgc gcgcagatca cgtccagcat ctcgaacgcg
      241 gtgtcgtcct cgggcaggtc ccggggcggg aacttcgcga acaccgtgtt cggctccagc
      301 gtcaggtggt agaccgacag gtgcggcggc tcgaaggcga gcgcctgcgc cagatcggcc
      361 tcgaactgcg cgagcgtctg accgggcagc gcgtacatca ggtcgaggtt gaaggtctcg
      421 aacgcgtcgc gtgcctcctc gaccgcggcc cgcgcctggc gggcgtcgtg cacgcggccg
      481 agcgcggcga gcttggcgtc gtcgaagctc tgcacgccga ccgacagccg cgtgacgccg
      541 gccgcgcggt agccgcggaa gcggtcgcgc tcgaaggtgc cggggttcgc ctcgagcgtg
      601 atctcgcaac ccgcctcgag cggaaggcgc gcacgaatgt cggcgatcag gcggtcgatc
      661 gccgcgggcg tgaacaggct gggcgtgccg ccgccgatga acaccgtctg cacgcgccgg
      721 ccccagatga agggcagcgc ggcttcgagg tcggcgcaca gcgcatcgag gtagcgcgcc
      781 tcggccaatt cgccctgcag ctcgtgcgaa ttgaagtcgc agtacgggca cttcttcagg
      841 caccacggca ggtgcacgta cagcgacagc ggcggcagcg cggcgagcga cagcgtgccg
      901 ccgcgcatgt agcgggcgag ccgctcggcg accggttcgg ccgcccgaac gtcgggttcc
      961 gacgcgatcc ggatttcagt caagcgacca agcctcacgc atcaacatca gcatctgcgc
     1021 cgccgcgagc gcgcggtggc tgtgggcgtt cttggtgtcg gcatcgagct cggcgacggt
     1081 cgcaccgagc gccggaatga acatcaacgg gtcgtagccg aaaccgcccg tgccgcgcgg
     1141 cgcgctcagg atctcgccga accagcgccc gaccgcgatc agcggctcgg ggtcgtccgc
     1201 cgagcgcacg gccaccagcg tgctgacgaa gcgcgcacgc cggtgggtcg cgccgtgcag
     1261 gcgttcgagc agcagcgcgt tgttcgccgc gtcctgcctc gcgcgttgcg tctcgcgatc
     1321 accgtcacgc ggcgacaagg ccgcgtagtg cgccgacgcc acgccgggcg cgccacccaa
     1381 cgcctcgacg cacaggccgg agtcgtcggc gagcgccgcc aggccggtgt ggcgcgcggc
     1441 gtggcgcgcc ttcgtcaagc cgttctcgat gaaggtgtgg aacggctcgt cggcctcgcc
     1501 gacaccgagc gcgccctgtg cgaccagctc gacgccgacc ggcgccagca gcgcctgcag
     1561 ttcgaccagc ttcttcgcgt tgttcgacgc cagcaccagg cgcatcgtca gaccccgagc
     1621 gccctgcgtt gcgccgcgat cagcgcgcgg atgccctgat cggccaggcc cagcagcgcc
     1681 cccatctcgt cgcgcgagaa cgccgcgccc tcggcggtgc cctgcacctc gacgaagccg
     1741 cccgcaccgg tcatcacgac gttcatgtcg gtgtcgcagg ccgagtcttc ggtgtattcg
     1801 agatcgagca gcggcgtgcc ctgaacgatg ccgaccgaga ccgcggcgac gaattcgcgg
     1861 atcggcgacg cactgagctt gccctgcgcc tgcagccacg tcaccgcgtc atgcgctgcg
     1921 acgaacgccc cggtgatcgc cgcggtgcgg gtgccgccgt cggcctggat cacgtcgcaa
     1981 tcaagcgaga tcgtgcgctc gccgagggca gccagatcga cgacacagcg cagcgagcgg
     2041 ccgatcaggc gctggatctc ctgcgtgcgg ccgctctgct tgccgcgcgc cgcttcgcgg
     2101 tcggtgcggg tatgggtgga gcggggcagc atgccgtact cggccgtcac ccaaccgctg
     2161 ccagagttgc gcaggaaggg ag
//
LOCUS       DATMJB010000016         2772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_530066, whole genome shotgun sequence.
ACCESSION   DATMJB010000016 DATMJB010000000
VERSION     DATMJB010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530066"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="VNU48_00340"
     CDS             <1..1251
                     /locus_tag="VNU48_00340"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF008453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HWI09756.1"
                     /translation="GVRARARTLELLTQVGIPQPETRLASYPHQLSGGQRQRVMIAMA
                     LANEPDLLIADEPTTALDVTVQAQILALLAEISARLGMSLLFITHDLGIVRRIADVVC
                     VMNSGKIVEQGPVEQVFTAPKHPYTKALLAAEPKPDPAPPRPNSPVVMSTTDLKVWFP
                     VRRGLLRKTVGHIKAVDGVSVSVRKGETLGVVGESGSGKTTLGLALLRLISSDGPIVF
                     LGKDIQGLRFKAMRPFRRDMQIVFQDPFGALSPRMSVGDIIAEGLSVHQATLSPEERE
                     ARVIKALKDVGMDPATRFRYPHEFSGGQRQRISVARAVVLEPNFVVLDEPTSALDMLF
                     QAHMVDLLRELQRKRDLTYMFISHDLRVVSSLASHLIVMRNGKVVEEGPASELFKNPK
                     SDYTRALFAAAFRIEAAPGGAPAT"
     gene            complement(1253..2095)
                     /locus_tag="VNU48_00345"
     CDS             complement(1253..2095)
                     /locus_tag="VNU48_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HWI09757.1"
                     /translation="MDDPRLTPARPDLAAKYLEGKVKAARFVTGEEFEIADAVAPLRE
                     AASSDAILLTQALKGERVTIYDRDSEGFAWGQLKSDGYVGWLPDRALVKPGAEPTHKI
                     TALRTFAFPGPSIKLPPSDTLPMGAAVTITRQDGVFAVTRDGHYLPLSHLGRIDRHAG
                     DFVAIAEQFVGTPYLWGGKSSLGIDCSGLVQIALNAAGTGCPRDSDMQQDGLGRALSP
                     TEFEKLQRGDLIFWKGHVAIVRDTDSIVHANAHHMMTAIEDTKKAIARIKAAGSEITS
                     IKRL"
     gene            complement(2088..>2772)
                     /locus_tag="VNU48_00350"
     CDS             complement(2088..>2772)
                     /locus_tag="VNU48_00350"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HWI09758.1"
                     /translation="TGGLDLKPSTGMLIMKKDMGGAANVLALALMVMDAKLKVRLRVL
                     IPAVENAVAGNAFRPLDIFKSRKGLTVEIGNTDAEGRLVLADALALADEDKPDLLIDL
                     GTLTGAARVALGPDLPPFYTDDETLALEIATCAKAENDPLWRMPLWPAYDAWLDSKVA
                     NINNAPSGAFAGSITCALFLQRFVEAAKSWLHVDIYGWTPSAKPARPEGGECQAARAI
                     YRLLDQRYG"
BASE COUNT          462 a          881 c          903 g          526 t
ORIGIN      
        1 ggcgtgaggg cgcgggcgcg aacgctcgaa ctgctcaccc aggtcggcat tccccagccg
       61 gaaacccggc ttgccagcta tccgcatcaa ttatccggtg gccagcgcca gcgcgtcatg
      121 atcgcgatgg cgctcgccaa cgagcctgac ctgctgatcg cggacgagcc caccaccgcg
      181 ctcgacgtca ccgtgcaggc gcagattctg gccttgctgg cggagataag cgcccggctc
      241 ggcatgagcc tgctgttcat cacccacgat ctcggcatcg ttcgccgtat cgccgacgtg
      301 gtctgtgtca tgaatagcgg caagatcgtg gagcaggggc cggtggagca ggtcttcacc
      361 gcgccaaaac atccttacac aaaagcgctg ctggcggcgg agccgaagcc ggatccggcg
      421 ccgccgcggc caaattcgcc ggtggtgatg tcgaccactg atttgaaagt ctggtttccg
      481 gtcaggcgcg gcctgttgcg caagacggtc ggccatatca aggcggtcga tggcgtcagt
      541 gtttccgtgc gcaagggcga gacgctgggc gtggtcggtg aatccgggtc cggcaagacc
      601 acgctcggac tggcgctgct gaggctgatc tcgtccgacg gcccgatcgt gtttctgggc
      661 aaggatatcc agggcctgcg tttcaaggcg atgcggccgt tccgccgcga catgcagatc
      721 gtgtttcagg atccgttcgg cgcgctgagc ccgcggatgt cggtcggcga tatcatcgcc
      781 gaaggcctca gcgttcatca agccacgctt tcaccggaag agcgcgaagc gcgcgtgatc
      841 aaggcgctga aggatgtcgg catggatccg gcgacccggt tccgttatcc gcatgagttc
      901 tccggcggcc agcgccagcg catcagcgtt gcccgcgcgg tggtgctgga gccgaacttt
      961 gtggtgctgg acgagccgac cagcgcgctc gatatgctgt tccaggcgca tatggtggat
     1021 ctgttgcgcg aactgcagcg caagcgcgat ctgacctaca tgttcatctc gcacgatctg
     1081 cgcgtggtct cctcactcgc cagccatctg atcgtgatgc gcaacggcaa ggtggtcgag
     1141 gaagggccgg cttcggagct gttcaaaaat ccgaagtccg actacacccg cgcgctgttc
     1201 gccgcagcgt tccggatcga ggccgcgccc ggtggcgctc ctgcgacctg actcacagcc
     1261 gcttgatcga cgtgatctcg ctgccagccg ctttgatgcg cgcgatggct tttttggtat
     1321 cttcgatcgc ggtcatcatg tgatgcgcgt tggcgtgaac gatgctgtcg gtatcgcgca
     1381 caatggcgac gtggcccttc cagaaaatca ggtcgccacg ctgcaatttt tcgaattccg
     1441 tcgggctcaa ggccctgccg aggccgtcct gttgcatgtc gctgtcgcgc gggcagccgg
     1501 tgccggcggc gttcaatgcg atctgcacca gaccggagca atcgatcccg agactgctct
     1561 tgccgcccca cagatagggc gtgccgacga attgctcggc gatcgcgacg aaatcgccgg
     1621 catggcggtc gatacggccg agatgcgata gcggcagata atggccatcg cgggtgacgg
     1681 cgaacacgcc gtcctggcgg gtgatcgtta ccgctgcgcc catcggcagc gtatcgctcg
     1741 gcggcagttt gatcgacggt cccggaaagg cgaatgtcct gagcgcggtt atcttgtgcg
     1801 tcggctcagc gccaggtttt accagcgcac gatcgggcag ccagccgaca tagccgtcgc
     1861 ttttgagctg gccccaggca aaaccttcac tgtcgcggtc gtaaatcgtg acgcgttcac
     1921 ccttgagcgc ctgcgtcagc agtatcgcgt cggaggacgc cgcctcgcgc agcggcgcga
     1981 cggcgtcggc gatctcgaat tcctcgccgg tgacaaagcg cgccgccttg accttgcctt
     2041 cgagatattt cgcggcaagg tccggccgtg ccggtgtcag gcgcggatca tccatagcgt
     2101 tggtccagca gcctgtagat cgcgcgcgcg gcctggcatt cgccgccctc gggccgcgcc
     2161 ggctttgccg atggcgtcca gccgtagatg tcgacgtgca gccagctttt cgcggcctcg
     2221 acgaagcgct gcaggaacag cgcgcaggtg atggagccgg cgaacgcgcc tgacggcgca
     2281 ttgttgatgt tggcgacttt cgagtcgagc caggcgtcgt aagccggcca tagcggcatt
     2341 cgccacaacg gatcgttctc cgctttcgcg caagtggcta tttcgagcgc cagcgtctcg
     2401 tcgtcggtgt agaagggcgg caaatccggg cctagcgcga cgcgtgccgc accggtcagg
     2461 gtgccgagat cgatcaggag atcgggcttg tcttcgtcgg ccagcgccag cgcatcggcc
     2521 agcaccagcc ggccctcggc gtcggtgttg ccgatctcga ccgtcaggcc cttgcgcgac
     2581 ttgaagatat cgagcggccg gaaggcattg ccggcgaccg cgttttcgac cgcgggaata
     2641 agaacccgca gccgcacctt cagcttcgcg tccatcacca tcagcgccag cgccagcacg
     2701 ttggccgcac cgcccatgtc cttcttcatg atcagcatgc cggtggacgg ttttagatcg
     2761 agacccccgg tg
//
LOCUS       DATMJB010000017         4237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_411676, whole genome shotgun sequence.
ACCESSION   DATMJB010000017 DATMJB010000000
VERSION     DATMJB010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411676"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VNU48_00355"
     CDS             complement(<1..948)
                     /locus_tag="VNU48_00355"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWI09759.1"
                     /translation="MVWVLGALCIGVLVLALAAYALTLGEIDEVLDDGLRQTALLLAD
                     RDLAGALPTRPVEHLASSADTESKLVAIARRRDGSLLFTSEPEMDLRFEFVAGQSLQT
                     ARDAHWHVFTLIQGERVVQVAQPVSVRRELAAESASQLLVPLLGLIAMIGAMLVLALR
                     RGLQPLNATTDALAQRGAASLAPLELDTVPKELLPMVRTLNDLLLRLGAAFEAQRHFV
                     ADAAHGLRSPVTALQLQVQLLERSGDPAQRLLATQELTAGIARTRRLIEQLLYLSRAA
                     ADESAGEIFTRAPVRLGDVARGVVARCSAEAERRGVDLGA"
     gene            complement(975..1631)
                     /locus_tag="VNU48_00360"
     CDS             complement(975..1631)
                     /locus_tag="VNU48_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI09760.1"
                     /translation="MRVLLIEDDAMIGRAAGRGLRQAGFTVDWVTDGPRGEAASANGT
                     YDAVILDLGLPRLDGMTLLRSLRARHDRVPVLIISARDAVSDRIAGLNAGADDYLLKP
                     FDLDELVARLRALIRRHAGAASGVLAVGALSLDPVGRNVTLDGRELTLTAKEFALLET
                     LLRRPGAVLSRERLEESIYGWGEEVGSNAVEVHLHNLRRKIGPTVICNVRGVGYKIVG
                     "
     gene            2049..2471
                     /locus_tag="VNU48_00365"
     CDS             2049..2471
                     /locus_tag="VNU48_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWI09761.1"
                     /translation="MLDKTSTKFSHVKPADTAWRSDGLRDFFLYKDLGVADATNGAVI
                     AHLVKANMAPEKGTGWHRHEADFQIVIMTKGWARFMYGDQVTLVEAGDCVHQRPGISH
                     YLFDYSPDMEYLEIVGPADFKSIDVPAVCEVPPPTPWA"
     gene            complement(2625..3362)
                     /locus_tag="VNU48_00370"
     CDS             complement(2625..3362)
                     /locus_tag="VNU48_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWI09762.1"
                     /translation="MPVATPSKPKSAPRAAAKPAAKARVARAPAATLTLSSKNYSSWS
                     LRGWLLARFVGLEFSEVMVSPDDADVRKELLLLAPSIRVPCLTHDGAKVWNTLAIAQY
                     LNEIKPDAGLMPDDRIARAHCRSVSGEMNSGFANLRASLPMNLKARFPGHKIWAGAQP
                     DIDRIVEIWTECLATYGGPFLFGQQRGMADAMYAPVATRFLTYDVKLPKPCILYCQTI
                     MAMPEMKEWVAAAKLEPDDIEELDMDF"
     gene            3485..>4237
                     /locus_tag="VNU48_00375"
     CDS             3485..>4237
                     /locus_tag="VNU48_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HWI09763.1"
                     /translation="MMYQAYQAQSDLMWPLRTLAKLSVPMLQDPGFGFAAQPSTRQIA
                     AACKVLELAEVTHRRPPWAIDAVLVKGERVPVVEQIVLTTPFASLLRFHKPGAPAQPK
                     VLVVAPMSGHFATLLRDTVRTVLQDHDVYVTDWYNVRDVPLAAGRFGLDEYTAHLIDF
                     LAAIGPGANVIAVCQPCVAALAAVSLMSEDGHPATPASLTLMAGPIDCRISPTEVNKL
                     ATTKPIGWFEKNLISHVPWRHRGGGRRVYPGFV"
BASE COUNT          664 a         1494 c         1482 g          597 t
ORIGIN      
        1 ggcgccgagg tcgacgccgc gccgctccgc ctcggcgctg cagcgcgcca ctacgccgcg
       61 cgcgacatcg cccaggcgca ccggcgcgcg cgtgaagatc tcgccggcgc tttcgtcggc
      121 cgcggcgcgc gacaggtaca gcagttgctc gatcaggcgg cgcgtgcgcg cgatgccggc
      181 cgtcaactcc tgcgtggcca gcaggcgctg cgccgggtcg ccgctgcgct cgagcagttg
      241 cacctgcagc tgcagcgccg tcaccggcga gcgcagcccg tgcgcggcgt cggcgacgaa
      301 atggcgctgc gcctcgaacg ccgcgcccag gcgcagcagc aggtcgttca gcgtgcgcac
      361 cattggcagc agttccttcg gcaccgtgtc gagctccagc ggcgccagcg acgccgcccc
      421 gcgctgcgcc agcgcgtcgg tggtcgcgtt cagtggctgc aggccgcgcc gcaaggccag
      481 caccagcatc gcgccgatca tcgcgatcag gccgagcagc ggaaccagca gctgcgacgc
      541 cgactccgcg gccagctcgc gccgcaccga caccggctgc gcgacctgca cgacacgctc
      601 gccctggatc agggtgaaca catgccagtg cgcatcgcgc gcggtctgca gcgactggcc
      661 ggcgacgaac tcgaagcgca ggtccatctc gggctccgag gtgaacagca gcgacccgtc
      721 ccggcgccga gcgatcgcaa ccagcttgga ctcggtgtcc gccgacgacg cgagatgctc
      781 caccggacgg gtcggcagcg cgcccgcgag gtcgcggtcg gccagcagca gcgcggtctg
      841 gcgcaggccg tcgtcgagca cctcgtcgat ctcacccagg gtcagcgcgt aggccgccag
      901 cgccagcacc agcacaccga tgcacagcgc acccagcacc cagaccatca ggtgacggcg
      961 tagcgacggc tgcctcatcc gacgatcttg tatccgaccc cacgcacgtt gcagatgacg
     1021 gtggggccga tcttgcgccg caggttgtgc aggtggacct cgaccgcgtt gctgcccacc
     1081 tcctcgcccc agccatagat cgactcctcg agccgctcgc gcgacagcac cgccccgggc
     1141 cggcgcagca gggtctcgag caacgcgaac tccttcgcgg tcagggtcag ctcgcgcccg
     1201 tccagcgtca cgttgcgccc gaccggatcg aggctgagcg cacccacagc caggacaccc
     1261 gacgccgcgc cggcatgacg ccggatcagc gcgcgcagcc tcgcgacgag ttcgtcgaga
     1321 tcgaagggct tgagcaggta gtcgtcggcg ccggcgttga gaccggcgat gcggtcgctc
     1381 accgcgtcgc gcgcgctgat gatgagcacc ggcacgcggt cgtggcgcgc gcgcaggctg
     1441 cgcagcagcg tcatgccgtc cagccgcggc aggccgaggt ccaggatcac cgcgtcgtag
     1501 gtgccgttgg ccgacgccgc ctcgccgcgg gggccgtcgg tgacccagtc cactgtgaag
     1561 ccggcctggc gcaggccacg gccggccgcc cggccgatca tcgcatcgtc ttcgatcagc
     1621 aggacacgca tcgtcgagag gcgcccgcag cgcccggcat gaaggtcgac ccaacgctgg
     1681 ccggccagca ggctcgcgta ggcggcgtcc tcgcgccgcg cgaagctggg caggtcgggc
     1741 gtcaccgagg cgtcagtatg aacgatgccg acgtcgcgcc cgatgatcac gtaggcgagg
     1801 aagcgcccgt cgggtgtcgg ctggggccgg cagcgcagca ggtgatcgcg gtaggtggcc
     1861 tcgaactcgg ccccggcggg ggtctcggtg atccggtcgg gcgcagtggg ggcacaggcg
     1921 gcggcgggcg cgccagcata ggcaggcgaa gttaagcagg cctgaagccg cagcccgccg
     1981 cccttgccgg ctggaacacc ttatgcatgc tgcgcctgca catttcggag tactgcagga
     2041 gtcgccccat gttggacaag acgagcacga agttctccca cgtcaagccg gccgataccg
     2101 cgtggcgcag cgacgggctg cgcgacttct tcctctacaa ggacctcggc gtggccgatg
     2161 cgacgaacgg cgctgtcatt gcgcatctgg tgaaggcaaa catggcaccg gaaaagggca
     2221 ccggctggca ccgccatgag gcggacttcc agatcgtcat catgaccaag ggctgggcca
     2281 ggttcatgta cggcgaccaa gtcacgctgg tcgaggccgg cgactgcgtg caccagcgcc
     2341 cgggcatcag ccactacctg ttcgactact cgcccgacat ggagtacctc gagatcgtcg
     2401 gcccggcaga cttcaagtcg atcgacgtgc ccgcggtgtg cgaggtgccg ccaccgacgc
     2461 cttgggcctg accagcacgc ggcgggccga gcgctgggcc gctccatggg ccacgaagag
     2521 gcccacttcg tggacctcgg cggcccgcat cccagggcca cgaaggggcc cctcgtggcc
     2581 cctggggatc gaccgatgta cccgtcggcc gaggggctcc attcctagaa atccatgtcc
     2641 agttcctcga tgtcgtcggg ctcgagcttc gcggcggcaa cccactcctt catctcgggc
     2701 atcgccatga tggtctggca gtacaggatg cagggcttgg gcagcttcac gtcgtaggtc
     2761 aggaagcggg tggcaaccgg ggcgtacatc gcgtcggcca tgccgcgctg ctggccgaac
     2821 aggaacgggc cgccgtaggt cgccaggcac tcggtccaga tctcgacgat gcggtcgatg
     2881 tcgggctggg cgccggccca gatcttgtgg ccgggaaacc gggccttcag gttcatcggc
     2941 agcgacgcgc gcaggttcgc gaagcccgag ttcatctcgc cgctgaccga gcggcaatgc
     3001 gcccgcgcga tgcggtcgtc cggcatcagg cccgcgtccg gcttgatctc gttcaggtac
     3061 tgcgcgatcg ccagcgtgtt ccagaccttc gcgccgtcgt gcgtcaggca gggcacgcga
     3121 atcgacgggg cgagcagcag cagttccttg cgaacgtcgg cgtcgtcggg cgagaccatc
     3181 acctcgctga attccaggcc cacgaaacgc gccagcagcc agccgcgcaa ggaccacgac
     3241 gaatagttct tgctgctcag cgtcagcgtg gccgcgggtg cgcgtgcgac cctggccttg
     3301 gccgcaggct tcgccgcagc cctgggcgcg ctcttgggct tggaaggggt cgcaacaggc
     3361 atgggggggc tccggcaccg atgggccggc tgtgaacagc aagggatgtg ccacaagccc
     3421 ggcgcggtgg ccgatggcgc ggtagttgca gtgaacagcg cgacgaatca gacccggaga
     3481 actcatgatg taccaggcct accaggcaca gtccgatctg atgtggccgc tgcgcaccct
     3541 ggcgaagctg tcggtgccga tgctgcagga cccgggcttc ggcttcgcgg cgcagccgtc
     3601 gacccggcag atcgccgcgg cctgcaaggt gctcgagttg gccgaggtca cgcaccggcg
     3661 cccgccctgg gccatcgacg cggtgctcgt gaagggcgag cgcgtaccgg tggtcgagca
     3721 gatcgtgctg acgacaccgt tcgcgagcct gctgcgcttt cacaagccgg gcgcgccggc
     3781 gcagccgaag gtgctggtcg tcgcgccgat gagcgggcac ttcgcgacgc tgctgcgcga
     3841 caccgtgcgc accgtgctgc aggaccacga cgtctacgtc accgactggt acaacgtgcg
     3901 cgatgtgccg ctcgccgcgg gccgcttcgg cctggacgag tacaccgcgc acctgatcga
     3961 ttttctggcc gcgatcggcc cgggcgcgaa cgtgatcgcg gtctgccagc cctgcgtcgc
     4021 ggcgctcgcg gcggtgtcgc tgatgtccga ggacgggcat ccggccacgc ccgcgagcct
     4081 gacgctgatg gccggcccga tcgattgccg catcagcccg accgaggtca acaagctggc
     4141 gaccacgaag ccgatcggct ggttcgagaa gaacctgatc agccacgtgc cgtggcgcca
     4201 ccgcggcggc ggccggcgcg tctacccggg cttcgtg
//
LOCUS       DATMJB010000018         2743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_234148, whole genome shotgun sequence.
ACCESSION   DATMJB010000018 DATMJB010000000
VERSION     DATMJB010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234148"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VNU48_00380"
     CDS             <1..233
                     /locus_tag="VNU48_00380"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011493726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWI09764.1"
                     /translation="GDTAGNLVYRKTARNFNPMMATASRMTVAEVEHLVPAGEIDPDH
                     IHTPGIFVKRIVEVGAGNKRIEFRNTRPRPAA"
     gene            334..984
                     /locus_tag="VNU48_00385"
     CDS             334..984
                     /locus_tag="VNU48_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HWI09765.1"
                     /translation="MAWTREQMAARAAKELRDGYYVNLGIGIPTLVSNYIPDGIDVSL
                     QSENGMLGMGPFPYEDEVDADLINAGKQTVSELPSTSYFSSAESFGMVRGGHIDLSIL
                     GAMQVAQNGDLANWMIPGKMVKGMGGAMDLVAGVKRVVVVMEHSAKDGAKLLKKCNLP
                     LTGEKVVDMVVTDLAVFTIDKHGKDGMALSELADGVTLDEVKSKTEAEFRVALKNA"
     gene            1001..1654
                     /locus_tag="VNU48_00390"
     CDS             1001..1654
                     /locus_tag="VNU48_00390"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI09766.1"
                     /translation="MITTMALAIRPGNADDADFIARTILLAQRGPRPRGWFDFALAQD
                     EPAVLAFLAKLAVAKPRSWYHVSQFLIAEVDGKPAAALCAMPSRETRDTMRSAIEEVA
                     VGAGMSASDLASIFARGAYARPCWIQGGDEDWLIEHVATQPSARGRGLVQALIAPALG
                     LGKAAGFSRASITFLIGNDAAERCYAKAGFVFADEKRDPAFEALTGAPGFRRFERGI"
     gene            complement(1679..>2743)
                     /locus_tag="VNU48_00395"
     CDS             complement(1679..>2743)
                     /locus_tag="VNU48_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWI09767.1"
                     /translation="RPALAVGLALVSLETLNDIGASEYLGVRTLTVSIFTTWLNRGSL
                     AGAAQLSCFMLAIVAGLIAIERYGRRNVTSEFSAESPRLTQRTAVTGLRGACAFAACL
                     LPVLLGFFVPLLFLLWQSFKRGLLTNFDVTLWRDAFNSVAFATLATLIALLLGFATIL
                     AWRFRPTAARSLAMNISQTGYTLPGLVLALGLLTPVLAIDNGLNALAGWIGHSLPGLI
                     LVGSGAAVVIAYVIRFLAVPTGFIKAGFERIPRDYDDSARAAGAGQATTMRQIHLPLL
                     RPAMLGAVIVVFVDCLKELPATLLLRPLNVDTLATSIYQYASRGSFEEGALAALLIVA
                     ASIGPVAWLTRFSDVPSGPA"
BASE COUNT          517 a          897 c          906 g          423 t
ORIGIN      
        1 agggcgatac cgccggaaat ctcgtgtacc gcaagaccgc gcgaaacttc aatccgatga
       61 tggcgaccgc ctcaaggatg accgtcgccg aggtcgagca tctggtgccg gcaggggaaa
      121 tcgatcccga ccacatccac acgcccggca tcttcgtcaa gcgcatcgtc gaggtcggcg
      181 ccggcaacaa gcgcatcgaa ttccgcaaca cccgcccgcg tcccgccgcg taagcatgac
      241 ccggacccgg agagccgcgc tcgcgcaaag tgggtagcgt tttttccgat aagatcatgc
      301 gcaaataaaa agcaacccag tcaggagagc cccatggcct ggacccgcga acagatggca
      361 gcgcgcgccg ccaaggaact gcgcgacggc tattacgtca atctcggcat cggcatcccg
      421 acgctggtct cgaactacat tcccgacggc atcgacgtca gtctccagag cgagaacggc
      481 atgctcggca tgggtccgtt cccgtacgag gacgaggtgg atgccgacct catcaacgcc
      541 ggcaagcaga cggtgagcga actgccgtcg accagctatt tttccagtgc cgaatccttc
      601 ggcatggtgc gcggcgggca tatcgacctg tcgatcctcg gggcgatgca ggtggcgcag
      661 aacggcgacc tcgccaactg gatgatcccg ggcaagatgg tcaagggcat gggcggcgcg
      721 atggacctgg tcgccggcgt caagcgcgtg gtggtggtga tggagcactc cgccaaggac
      781 ggcgccaagc tgttgaagaa atgcaacctg ccgctgaccg gcgagaaggt ggtcgacatg
      841 gtggtgacgg atctcgcggt cttcaccatc gacaaacacg gcaaggacgg catggcgctg
      901 agcgagctcg ccgacggcgt cacgctcgac gaggtgaagt cgaagaccga ggccgaattc
      961 cgggtggcgc tgaagaacgc ctgagggcgg atggcgacgg gtgatcacaa ccatggcgct
     1021 ggcgatacgc ccgggaaacg ctgacgacgc cgatttcatc gcccgcacca ttttgctggc
     1081 gcaacgcggt ccccggcctc gcggctggtt tgacttcgcg ctggctcaag acgaacctgc
     1141 ggtgcttgcg tttctcgcaa agcttgcggt cgcaaaaccg cgctcctggt accatgtctc
     1201 gcaattcctg atcgccgagg tcgatggaaa gccggcggcc gcgctctgcg ccatgccgtc
     1261 gcgcgagacg cgcgacacca tgcgttctgc gatcgaggaa gtggcggtcg gcgccggaat
     1321 gagcgcttcg gatctcgcct cgatctttgc gcggggcgcc tatgcccgcc cctgctggat
     1381 ccagggcgga gacgaggact ggctgatcga gcacgtggcg acgcagccgt ccgcgcgcgg
     1441 acgcggcctt gttcaggccc tgatcgcgcc cgcccttggc ctcggcaagg cggcgggttt
     1501 ttcacgggcc tcgatcactt tcctgatcgg caatgacgcc gcggagcgat gctacgccaa
     1561 ggccggtttt gtcttcgccg atgaaaaacg cgatccggcc ttcgaggcgc tgaccggcgc
     1621 gccgggcttt cgccggttcg agcgcgggat ctgaacttcg tgtccgctgt gcgggctctt
     1681 acgccggccc gctcggcacg tcggaaaacc gcgtcagcca cgccaccggg ccgatgctgg
     1741 cggcgacgat caacagcgcg gcgagcgcgc cttcctcgaa actgccgcgg ctggcgtact
     1801 ggtagatcga ggtcgccagc gtgtcgacat tgagcggccg cagcagcagc gtggccggca
     1861 attccttcag gcaatccacg aacacgacga tgacggcgcc gagcatcgcc ggccgcagca
     1921 gcggcaggtg gatctgccgc atcgtggtgg cttgtccggc gccggcggcg cgcgcgctgt
     1981 cgtcgtagtc gcgcgggatc cgctcgaatc ccgccttgat gaacccggtc ggcaccgcca
     2041 ggaaccggat cacataggcg atcacaacgg cagcacccga gccgaccagg atcagtcccg
     2101 gcagcgaatg tccgatccat ccggcgagcg cgttcagtcc gttgtcgatc gccagcaccg
     2161 gcgtcagcag gccgagcgcc agcaccagtc ccggcagcgt gtagccggtt tgcgagatgt
     2221 tcatcgccag cgaccgcgcc gcggtcggcc ggaatcgcca ggccaggatg gtggcaaagc
     2281 cgagcagcag tgcgatcagc gtggcgaggg ttgcgaacgc gaccgagttg aaggcgtcgc
     2341 gccacaacgt cacgtcgaaa ttcgtcagca ggccgcgttt gaaactctgc cacagcagaa
     2401 acagcagcgg cacgaagaac ccgagcagca ccggcaacag gcaggctgcg aaggcgcaag
     2461 cgcccctcaa gcccgtcacc gccgttcgct gcgtcagccg cgggctttcg gctgagaact
     2521 ctgacgtgac attgcggcgg ccgtagcgct cgatcgcgat caggccggcc acgatcgcca
     2581 gcatgaagca ggacaattgc gcggcgcccg caaggctgcc gcggttgagc caggtcgtga
     2641 agatggacac ggtcagcgtc cgcaccccga gatattcgct ggcgccgatg tcattcaacg
     2701 tttccagcga taccagcgcg aggcccaccg ccagcgccgg ccg
//
LOCUS       DATMJB010000019         4216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_115265, whole genome shotgun sequence.
ACCESSION   DATMJB010000019 DATMJB010000000
VERSION     DATMJB010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115265"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VNU48_00400"
     CDS             <1..479
                     /locus_tag="VNU48_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09768.1"
                     /translation="GPVIVDARSGNEPHDIDAVMKAMGFALESATTGPFDEWLAYYRR
                     PVTITNAPPAGMVPTGVVLTEGDAAADMAGEPRPDKPGRSSWAEASAEPAPAEPEAKP
                     PEQAAAEEADRVAETGDTTPSVDPTEPPPNQGEGIHTGQPLLGARRSRAKKSDDAA"
     gene            482..979
                     /locus_tag="VNU48_00405"
     CDS             482..979
                     /locus_tag="VNU48_00405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HWI09769.1"
                     /translation="MALKLHSDLERFSLKCEYVEATGCWQWTGSLDKDGYARRSKVGS
                     RTDGTRREVSPHRWFYEEFVGPIPAGLVTDHLCRNRACVNPWHIEPVTALVNHKRGER
                     ALATVCSKGHLILGANEVKRRGGHRCRICHNAYLAAYHRSTGHRHSRAYRERKRSEKS
                     APATF"
     gene            979..1701
                     /locus_tag="VNU48_00410"
     CDS             979..1701
                     /locus_tag="VNU48_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09770.1"
                     /translation="MATSQVRTLNERTHRPVVVVNRMMKPQWHEVFENNPRIARAVTR
                     NTVQLVNAGGARPYIVSKSATHWKWRPWDIAPGEIYLSAGENEFAAPYAGRILIEPHT
                     KVEGSNKSWVWERWQQLVDRGGDFIQVGPHGTVRLDGVEFVETPSFRMACAVLAVSRA
                     FVGTEGGLGHAAAALGVPAVVLFSAFISPAVTGYQSHRNIYKGAFGLGCGARMPCACC
                     QISMAAISVDEVETNLKEILNG"
     gene            1694..2200
                     /locus_tag="VNU48_00415"
     CDS             1694..2200
                     /locus_tag="VNU48_00415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI09771.1"
                     /translation="MGKPCNAATYRKAAFDLALANSPKTVVEVGVYAGALSRMFAELP
                     TLERQFIVDSWEGGYSGFSQDHMDAIGAEVKAWGATQPKLTIHHMRSADAAALFEDES
                     IDFFHTDGDHSLEGITTDIRLWLPKVKTGSILSGDNYEIPAVAAGVKKMLPQHKLLAN
                     GRLWFAVK"
     gene            2197..2922
                     /locus_tag="VNU48_00420"
     CDS             2197..2922
                     /locus_tag="VNU48_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09772.1"
                     /translation="MNTAFKFAEGAGQVLIGRHELLGGLDCEQVFKDAGLLAIDGIAW
                     DADMSVIRSALVNKKRAFKAPFIEHEPSEAVSDELHRLGDHARRLFAELYPTFTATEV
                     RTSFRPMITGPEPLHFDSYGGESPMVTAYINVSDEPRVYGIGPNFPTLVETQPMFMHS
                     LAKDVGMDLSYALRQRTASGLGPLGPDAPRHRVEFAPGAIWFFNAKTVSHEVIHGTGA
                     FGISWEVPGCGAPMQADLLEKLA"
     gene            2919..3242
                     /locus_tag="VNU48_00425"
     CDS             2919..3242
                     /locus_tag="VNU48_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09773.1"
                     /translation="MSKLTKDDVRILRWACESQIAAIDANPSGDLLYEEEFKLLRALR
                     NLPKVKELPPAPAMPDERTIEKVLLKAQRKRAPITVDYSRGALDLPRGLATAKHLMKK
                     LDMHA"
     gene            3239..3886
                     /locus_tag="VNU48_00430"
     CDS             3239..3886
                     /locus_tag="VNU48_00430"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWI09774.1"
                     /translation="MKLAHGWHFPNGEKHMIDWLAEPKNKLILNGRESYQGKKQVAAI
                     AHCTNRRVAVDIGSHIGLWTHNLAAAFESVQAFEPVAAHRECFTANIHASNVELNACA
                     LGHEMGMVNIWSNPTSSGDSWVSGTGDIPMHTLDSFELECVDLLKIDCEGFELNVLRG
                     AVETIARCKPVICVEQKRDMAVKFGLKPQGAIEFLQSRGYRVAEVLSGDYIMVPA"
     gene            3883..4101
                     /locus_tag="VNU48_00435"
     CDS             3883..4101
                     /locus_tag="VNU48_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09775.1"
                     /translation="MKPLPLIRRIDPDLRIGDAVQLGVDTPVMTVYDRMAGDWICCNY
                     VKGTDMTLIAVPADALQRVERATQGKPA"
BASE COUNT          818 a         1343 c         1384 g          671 t
ORIGIN      
        1 acggcccggt gatcgtcgac gcgcgcagcg gcaacgagcc gcacgacatc gacgcagtga
       61 tgaaggccat gggcttcgcg ctcgagtcgg cgaccacggg cccgttcgat gaatggctgg
      121 cctactaccg tcggccagtc acgatcacca acgcgccgcc ggcaggcatg gtgccgactg
      181 gcgtcgtgct gacggagggc gacgccgctg cagacatggc cggcgagccg cgacctgaca
      241 aaccgggccg ctcgtcgtgg gccgaagcgt cggcagagcc cgcgcccgcc gaacccgaag
      301 ccaagccgcc cgagcaggcc gcagccgaag aggccgaccg cgtcgccgag accggcgaca
      361 ccacgccgag cgtcgacccg accgagccgc cgccgaacca aggcgagggc atccacaccg
      421 gccagccact gctgggtgcg cgccgcagcc gagcgaagaa gtccgacgac gcagcctgat
      481 cgtggcgctg aagctgcact cggaccttga gcgtttctcg ctcaagtgcg agtacgtcga
      541 agcgaccggt tgctggcaat ggaccggcag tctggacaag gacggatacg cgcgccgctc
      601 gaaagtcggt agccgcaccg atggaacgag gcgcgaagtg agtccgcatc gctggttcta
      661 cgaagagttc gtcggaccga tccctgccgg gttggtcacg gaccacctgt gccgcaaccg
      721 tgcctgcgtg aacccttggc acatcgagcc tgtgaccgcg ctggtgaacc acaagcgcgg
      781 cgagcgcgca ctcgccacag tctgctccaa gggccacctc atcctcgggg cgaacgaagt
      841 gaagcgtcgc ggcggacacc gctgcaggat ctgccacaac gcgtacctcg cggcatacca
      901 tcgctccacg ggtcatcggc attccagagc gtatcgggag cgcaagagga gcgagaaatc
      961 ggcgccggcg actttctgat ggcgacctcg caggtgcgca cgctcaacga gcgcactcac
     1021 cgccccgtgg tggtggtcaa ccggatgatg aagcctcagt ggcacgaggt cttcgagaac
     1081 aacccgcgca tcgcgcgcgc cgtgacgcgc aacaccgtgc agttggtcaa cgctggcgga
     1141 gctcgcccgt acatcgtgtc caagagcgcg acgcactgga aatggcgtcc ttgggacatc
     1201 gcacccggcg aaatctacct gagcgccggc gagaatgaat tcgcggcgcc atacgccggc
     1261 cgcatcctga tcgagccgca cacgaaagtg gaggggtcga ataaatcctg ggtgtgggag
     1321 cgctggcagc agctggtcga ccgcggcgga gacttcatcc aggtaggccc gcacggcacc
     1381 gtgcgtctgg acggcgtgga gttcgtcgag acgccgagct tccgcatggc ctgcgcggta
     1441 ctcgcggtga gccgggcgtt tgtcggcacc gagggtggac tcggacacgc ggccgctgca
     1501 ctcggcgtgc cggctgtggt gctcttcagc gccttcatct cgccggcggt gacgggatac
     1561 cagtcccacc gaaacatcta caagggcgcc ttcggtctgg gctgcggagc gcgcatgccg
     1621 tgcgcctgct gccagatctc gatggcggcg atcagtgttg acgaagtgga aaccaacttg
     1681 aaggaaattc tgaatgggta agccatgcaa cgccgccaca taccgcaagg ccgcgtttga
     1741 cctcgcgctg gcgaactcgc cgaagaccgt cgtggaggtc ggcgtctacg ccggcgcgct
     1801 gtcgcgcatg ttcgccgagc tgccgacgct cgagcgtcag ttcatcgtgg attcgtggga
     1861 gggcggctac agcggcttct cgcaggacca catggacgcc atcggcgccg aggtgaaggc
     1921 ctggggcgcg acgcagccga agctcaccat ccatcacatg cgctcggctg atgcggctgc
     1981 gctgttcgaa gacgagtcga tcgacttctt ccacaccgat ggcgatcact cgctcgaggg
     2041 catcacgacc gacatcaggc tgtggctgcc gaaggtcaag accggatcga tcctgtcggg
     2101 tgacaactac gagataccgg ccgtcgcggc tggcgtcaag aagatgttgc cgcagcacaa
     2161 gttgctggcc aacggtcggc tctggttcgc cgtgaaatga acacggcatt caagttcgct
     2221 gaaggcgccg gtcaagttct catcggccgg cacgagttgc tgggcggact cgactgcgag
     2281 caagtgttca aggatgccgg cctgctcgcg atcgacggca tcgcgtggga cgccgacatg
     2341 agcgtgatcc gctcggcgct cgtcaacaaa aagcgggcgt tcaaggcgcc gttcatcgaa
     2401 cacgagccgt ccgaagccgt gagcgatgaa ctgcaccgtc ttggcgatca cgcgcgccgg
     2461 cttttcgccg agctctaccc cacgttcacg gccaccgagg tccgcacgtc gttccggccg
     2521 atgatcacgg gccccgagcc gctgcacttc gacagctatg gcggcgagtc gccgatggtg
     2581 acggcctaca tcaacgtatc cgacgagccg cgtgtctacg gcatcgggcc caacttcccg
     2641 acgctggtgg agacgcagcc gatgttcatg cacagtctcg cgaaagacgt tggaatggac
     2701 ctcagctatg cgctgcgcca gcgcaccgct tcaggcctcg ggccgctggg gcccgacgcg
     2761 ccgcggcacc gcgtcgagtt cgcgccgggc gccatctggt tcttcaacgc gaagaccgtg
     2821 agccacgagg tgatccacgg caccggtgca ttcgggatct cgtgggaggt gccgggctgc
     2881 ggagcgccga tgcaggcgga tcttctggag aagttggcat gagcaagttg accaaggacg
     2941 atgttcgcat cttgcgctgg gcatgcgagt cgcagatcgc ggcgattgac gcgaatccgt
     3001 cgggcgatct tctgtacgaa gaggaattca aactcctgcg ggcgctgcgg aacctgccga
     3061 aggtgaagga gttgccgcca gcgccggcca tgcctgacga gcgcacgatc gagaaggtcc
     3121 tgctcaaggc gcagcgcaag cgcgcgccca tcaccgtcga ctacagccgg ggtgcactcg
     3181 atctcccgag agggctcgcc acggcgaagc atctgatgaa gaaactggac atgcacgcat
     3241 gaagctcgcc cacggttggc actttccaaa tggcgagaag cacatgatcg actggctcgc
     3301 cgagccaaag aacaagttga ttctgaacgg tcgcgaaagc taccaaggga aaaaacaggt
     3361 cgccgcgatc gcgcactgca caaacaggcg cgtggcggtt gacatcggct cccacatcgg
     3421 cctgtggacg cacaacctgg cggcggcatt cgagtccgtg caggccttcg agccggtggc
     3481 cgcacaccgt gagtgtttca cggcgaacat ccacgcgtcg aacgtcgagc tcaacgcatg
     3541 cgcgctcggt cacgagatgg gtatggtcaa catatggtcg aacccgacga gctccggtga
     3601 ttcatgggtc agcggcaccg gcgacatccc gatgcacacg ctggacagct tcgagctcga
     3661 gtgcgtagac ctgctgaaga tcgactgcga aggattcgag ttgaacgtcc tgcgcggcgc
     3721 tgtcgaaacg atcgcgcgct gcaagccggt gatctgcgtc gagcaaaagc gcgacatggc
     3781 cgtcaagttc ggcctcaagc cacaaggcgc catcgagttc ctgcagagcc gtggctaccg
     3841 cgtggccgaa gttctgagcg gcgactacat catggtcccg gcatgaagcc gctaccgctg
     3901 attcgtcgca tcgatcccga cctgcgcatc ggcgacgccg ttcaactcgg cgtcgacacg
     3961 ccggtgatga ctgtctacga ccgcatggcc ggcgattgga tctgctgcaa ctacgtgaag
     4021 ggcaccgaca tgacgctgat cgccgtgcct gccgatgcgt tgcagcgcgt cgagcgcgca
     4081 acgcaaggga agccggcatg aagatcacga tcgaactcga ccacgccgag tgcgtgatgc
     4141 tggcttcggc gctttctttc tacccatatt tcaggagcga agatgaagag gtggccgcat
     4201 gcgttctgag ccgccg
//
LOCUS       DATMJB010000020         2199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_588751, whole genome shotgun sequence.
ACCESSION   DATMJB010000020 DATMJB010000000
VERSION     DATMJB010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588751"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /gene="cysW"
                     /locus_tag="VNU48_00440"
     CDS             <1..676
                     /gene="cysW"
                     /locus_tag="VNU48_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HWI09776.1"
                     /translation="AIWLTLIAAAISVPLNLVFGVAAAWCIAKFDFRGKNVLLTLIDL
                     PFSVSPVIAGLIYVLIFGLQGWLGESLRDHDLKVIFAVPGIVLATIFVTFPFIARELI
                     PLMQAQGQEQEEAARVLGASGWQMFWRVTLPNVKWALLYGVILCNARAMGEFGAVSVV
                     SGHIRGQTNTMPLHIEILYNEYQFAAAFAVASLLALLALVTLVLKYTVEQRVKKQKRS
                     ASDRDE"
     gene            701..1771
                     /locus_tag="VNU48_00445"
     CDS             701..1771
                     /locus_tag="VNU48_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HWI09777.1"
                     /translation="MSIEIRHLNKRFGKTVVCDNLNLDIPSGELVALLGPSGSGKTSL
                     LRIIAGLERPDSGSVLFHGEDATFDDVRDRQVGFVFQHYALFGHMSIFENVAFGLRVR
                     PKATRPSEARITAKVTELLELVQLDWLADRYPHQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRRWLRRLHDEMHITSVFVTHDQDEAMEVADRVVVMNQGRIEQD
                     GAPDEVYDHPATPFVLQFLGDVNLFHGRFGHAPGGGTAPAEEVSYVRPHELEIVAEAQ
                     EGALAVTLSQALTVGPQTRIEFKRLDDGSYVDVEMTRGDYTALRDRLGLRNGSQVHLK
                     PRRITRFSAGASEFVDDPAAMI"
     gene            complement(1862..>2199)
                     /locus_tag="VNU48_00450"
     CDS             complement(1862..>2199)
                     /locus_tag="VNU48_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWI09778.1"
                     /translation="RTHIDEAFDKAGIEIDLVITAVDADVIKTYVGLGLGVGIVASIA
                     FDDERDRDLRAIDARHLFEINLTKLAVRRGSFLRTFVYDFIETFASSLKREVVERAIA
                     TAPGTRIEL"
BASE COUNT          351 a          710 c          763 g          375 t
ORIGIN      
        1 ggcgatctgg ttgacgctga tcgcagcggc gatctcggtg ccgctgaacc tggtgttcgg
       61 cgttgcggcc gcatggtgca tcgccaaatt cgatttccgc ggcaagaacg tgctgctgac
      121 gctgatcgac ctgccgttct cggtctcgcc ggtgatcgcc ggcctgatct acgtcttgat
      181 cttcggcttg cagggctggc tcggcgagtc gctgcgcgac cacgacctga aggtgatctt
      241 cgcggtgccg ggcatcgtgc tcgcgacgat cttcgtgacc ttcccgttca tcgcgcgcga
      301 actgattccg ttgatgcagg cgcaggggca ggagcaggag gaagcggcac gtgtgctcgg
      361 cgcctccggc tggcagatgt tctggcgcgt cacgctgccc aatgtgaaat gggcgctgct
      421 gtacggcgtg atcctgtgca acgcccgcgc gatgggcgag ttcggtgccg tcagcgtggt
      481 ctcgggccac atccgcggcc agaccaacac gatgccgctg cacatcgaga ttctctacaa
      541 cgagtaccag ttcgccgcgg ccttcgcggt ggcgtcgttg ctggcgctgc tggcgctggt
      601 gacgctggtg ttgaagtaca cggtcgagca acgcgtgaag aagcagaagc gcagcgcgtc
      661 ggacagagat gagtgaggaa cctgccatga gcatcgacgt atgagcatcg aaatccgcca
      721 cctgaacaag cgcttcggca agaccgtcgt ctgcgacaac ctgaatctgg acatcccgtc
      781 gggcgagctg gtcgcgctgc tcgggccttc gggctcgggc aagacctcgc tgctgcgcat
      841 catcgcgggg ctcgagcggc cggattccgg gtcggtgctg ttccacggcg aggacgcgac
      901 cttcgacgat gtgcgcgatc gccaggtcgg attcgtgttc cagcactacg cgctgttcgg
      961 tcacatgagc atcttcgaga acgtggcctt cggcctgcgc gtgcgaccga aggcgacgcg
     1021 cccgagtgaa gccaggatca ccgcgaaggt caccgagctg ctcgagctcg tgcagctcga
     1081 ctggctggcg gatcgctacc cgcatcaact gtcgggcggc cagcgccaac gcatcgcgct
     1141 ggcgcgtgcg ctcgcggtcg agcccaaggt gctgctgctc gacgaaccct tcggcgcgct
     1201 cgacgcgaag gtgcgcaagg agttgcgccg ctggcttcgc cgcctgcacg acgagatgca
     1261 catcacgagc gtgttcgtca cccatgacca ggacgaggcg atggaggtcg ccgaccgcgt
     1321 ggtcgtgatg aaccagggcc ggatcgagca ggacggcgcg ccggacgagg tctacgacca
     1381 cccggccaca cccttcgtgc tgcagttcct cggcgacgtg aatctgttcc acggccgctt
     1441 cggccacgcg ccgggtggtg gcacggcgcc tgccgaagaa gtcagctacg tgcgcccgca
     1501 cgaactcgag atcgtcgccg aggcccagga aggcgcgctc gcggtgacct tgtcgcaggc
     1561 gctgacggtg gggccgcaga cgcgcatcga gttcaagcgc ctcgacgacg gcagctacgt
     1621 cgatgtcgag atgacgcgcg gcgactacac cgccttgcgc gatcggctcg ggttgcgcaa
     1681 tggcagccag gtgcacctga agccgcggcg tatcacgcgc ttctcggccg gcgcatcgga
     1741 attcgtcgac gacccggccg cgatgatctg aagggatggt gcccccggtc gcgcgggtac
     1801 gcacgaccac cccccgaggg ggcgccggcc gctgcgcagc ggtcgcgcgc tggccggcgc
     1861 gtcagagctc gatgcgggtg ccgggggcgg tcgcgatcgc gcgctcgacg acctcgcgct
     1921 tcagcgacga tgcgaaggtt tcgatgaagt cgtagacgaa agtgcgcagg aagctgccgc
     1981 ggcgcaccgc cagcttggtc aggttgatct cgaacaggtg gcgcgcatcg atcgcgcgca
     2041 ggtcgcggtc gcgctcgtcg tcgaaggcga tcgaggcgac gatgcccacc cccaggccca
     2101 ggcccacgta ggtcttgatc acgtccgcgt ccaccgcggt gatcaccagg tcgatctcga
     2161 tgccggcctt gtcgaaggcc tcgtcgatgt gcgtgcgcc
//
LOCUS       DATMJB010000021         3655 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_470706, whole genome shotgun sequence.
ACCESSION   DATMJB010000021 DATMJB010000000
VERSION     DATMJB010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470706"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..292)
                     /gene="rpmB"
                     /locus_tag="VNU48_00455"
     CDS             complement(59..292)
                     /gene="rpmB"
                     /locus_tag="VNU48_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HWI09779.1"
                     /translation="MARVCQVTGKRPMVGNNVSHANNKTKRRFLPNLQYRRFWVEAEN
                     RWVRLRISNAALRLIDKVGIEKVLVDLRARGEI"
     gene            complement(399..1349)
                     /gene="trxB"
                     /locus_tag="VNU48_00460"
     CDS             complement(399..1349)
                     /gene="trxB"
                     /locus_tag="VNU48_00460"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWI09780.1"
                     /translation="MSTPTQHARVLILGSGPAGYTAAVYAARANLKPVLITGIAQGGQ
                     LMTTTDVDNWPADVDGVMGPDLMQRFQKHAERFNTQIVFDHINTVDLGQRPFTLTGDS
                     GRYTCDALIIATGASAKYIGLPSEEAFMGRGVSGCATCDGFFYRDQAVCVVGGGNTAV
                     EEALYLSNIASKVHLIHRRDKFRAEPILVDKLHAKVAEGKMELHLWNTLDEVLGDASG
                     VTGVRLKSTRDGGTSELELTGCFIAIGHEPNTGIFKGQLEMKDGYIISKSGLAGMATM
                     TSVPGVFAAGDVQDHVYRQAITSAGTGCMAALDAQRFLEQ"
     gene            1596..2261
                     /locus_tag="VNU48_00465"
     CDS             1596..2261
                     /locus_tag="VNU48_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWI09781.1"
                     /translation="MAMLSNLDLIRRVPLFSLLTNDQAQGIADSVVKRRFRRGEIVVE
                     HGKKSNALFILLTGRARVLTADQRGREVILAVLQPGDYVGEMSLIDNEPHSATVRAEV
                     QTDMLILGRAEFARCLPENSSLSYAIMRGLVYRLRAADRQIESLALLDVYGRVARTLL
                     DMCEMEGEVKLIRNKVSRQDLAKVVGASREMVSRVMKDLEERGMIQTQENGSVIIKEQ
                     LTA"
     gene            2307..>3655
                     /locus_tag="VNU48_00470"
     CDS             2307..>3655
                     /locus_tag="VNU48_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HWI09782.1"
                     /translation="MTFPLGSLREGGGASSSFVPSAAGPKRDGARGAAVPHDPAGWRA
                     QLGLLIGAVLWLLAVLALATHNPADAAFSTSGTATVTLNKAGALGAWFSDLAYFVFGY
                     SVWWAVAVALRAWLGGLARVLRTDRAAPAVPLHAPRPVWLFWVGLALLLAASASLEWT
                     RLYQWEGRVAGGHAGGALGYTLGSASQRLIGFAGSGVLWIAALVAGIALALRFSWLRL
                     AEAIGAWAESLRERRALRTERAEDARVGERALREREVIVNVEHQLHEDHLPIVIEAPV
                     FEVPKSERVVKERQKPLFVELADTRLPQVDLLDASPGRTETVSPETLEMTSRLIEKKL
                     KDFGVEVRVVAASPGPVITRYEIEPATGVKGAQVLNLSKDLARSLSLVSIRVVETIPG
                     KTTMALELPNPKRQTIRLSEILGSTVYNDASSQLTMGLGKDIVGNPVVADLAKMPHIL
                     "
BASE COUNT          565 a         1221 c         1249 g          620 t
ORIGIN      
        1 ccggcgctgg attccagctt gatcttttcg cgtccgcctt ttgccatgat cgttctcctt
       61 agatttcgcc gcgggcgcgc aggtcgacca ggaccttctc gatgcccacc ttgtcgatca
      121 ggcgcaaggc cgcattggaa atgcgcaacc gcacccagcg attctcggcc tcgacccaga
      181 agcggcggta ctgcaagttg ggcaggaagc ggcgcttcgt tttgttgttc gcgtgggaga
      241 cgttgttccc caccatcggg cgcttgcccg tgacctgaca gacgcgtgcc atgacgcttc
      301 tccaatattg tttgacccgc tctcgtgaaa gagccgccgg aatttcggca aagactgcga
      361 ttatagccag attcccgtta ccggaaaagc cgccgcttct attgctccag gaagcgctgc
      421 gcgtccagcg ccgccatgca gccggtgcct gcgctggtga tggcctggcg gtacacgtgg
      481 tcctgcacgt cgccggccgc gaacacgccg ggcacgctgg tcatcgtcgc catgccggcc
      541 agcccgctct tcgagatgat gtagccgtcc ttcatctcga gctggccctt gaagatgccg
      601 gtgttcggct cgtggccgat cgcgatgaag cagccggtca gctcgagctc ggaggtgccg
      661 ccgtcccgcg tgctcttcag ccgcacgccg gtcacgccgg acgcgtcgcc cagcacctcg
      721 tccagcgtgt tccaaagatg cagttccatc ttgccttcgg ccaccttcgc gtgcagcttg
      781 tcgaccagga tcggctcggc gcggaacttg tcgcgtcggt ggatcaggtg caccttgctg
      841 gcgatgttgg acaggtacag cgcctcctcg acggcggtgt tgccgccgcc gacgacgcac
      901 acggcctgat cgcggtagaa gaagccgtcg caggtcgcgc agccgctgac gccgcggccc
      961 atgaaggcct cttccgacgg cagcccgatg tacttggccg aggcgccggt ggcgatgatc
     1021 aatgcgtcgc aggtgtagcg gcctgaatca ccggtcagcg tgaacggccg ctggcccagg
     1081 tcgaccgtgt tgatgtggtc gaacacgatc tgcgtgttga aacgctcggc atgcttctgg
     1141 aagcgctgca tcaggtccgg gcccatcaca ccgtcgacat cggccggcca gttgtcgaca
     1201 tcggtggtgg tcatcagctg gccgccctgc gcgatgccgg tgatcagcac cggcttcagg
     1261 ttggcgcgtg cggcgtagac cgcggcggtg tagccggcgg ggcccgaacc gaggatcagg
     1321 acgcgggcat gttgggtggg tgtgctcatg acgggggcag ttgggcgatg aggggcagac
     1381 gctggcgcgc agcggccttg atgcgtaagt ctgacacggc gttcgatcgg gctgacgcat
     1441 gggccgttaa tggtgacaat agccaaattg taggcagcgt gagaatttgg cagaaccgct
     1501 gccgcaagcg cgagaccgag gcaacaggcg cttgccaggc gtttcgcgag ccccatggca
     1561 gggcttcgtc tgcaacagaa agaagggcaa accgcatggc aatgctttcc aatctcgatc
     1621 tgatccggcg tgtgccgctg ttttcactgc tgaccaacga ccaggcgcag ggcatcgccg
     1681 acagcgtcgt caagcgccgc ttccggcgcg gcgagatcgt ggtggagcat ggcaagaagt
     1741 cgaacgcgct gttcatcctg ctgaccggcc gggcccgcgt gctgacggcc gaccagcgcg
     1801 gccgcgaggt gatcctcgcg gtgttgcagc cgggcgacta cgtcggcgag atgagcctga
     1861 tcgacaacga gccccattcg gccaccgtgc gcgccgaggt ccagaccgac atgctgatcc
     1921 tcggccgcgc cgagttcgcg cgctgcctgc ccgagaattc gagcctgtcg tacgcgatca
     1981 tgcgcggcct cgtgtaccgg ctgcgcgctg ccgaccggca gatcgaatcg ctcgcgctgc
     2041 tcgacgtcta tgggcgcgtc gcccgcaccc tgctcgacat gtgcgagatg gaaggcgagg
     2101 tcaagctgat ccgcaacaag gtctcgcgcc aggacctggc caaggtcgtc ggcgcatcgc
     2161 gcgagatggt cagccgcgtc atgaaagacc tcgaagagcg gggcatgatc cagacccagg
     2221 aaaacggctc cgtgatcatc aaggaacagc tcaccgcctg atcgggcgcc gcgcgaatcg
     2281 ccgtcgcgat cggccacaat ccgccgatga cctttcccct cggttcgctg cgcgaaggcg
     2341 gcggtgcttc cagcagtttc gttccctccg ccgccggccc caagcgagac ggcgcgcgcg
     2401 gcgccgccgt gccgcacgac ccggccggct ggcgcgcgca actcggcctg ctgatcggcg
     2461 cggtgctctg gctgctggca gtgctggcgc tggccacgca caacccggcc gacgcggcgt
     2521 tctcgacctc gggcaccgcc accgtcacgc tgaacaaggc cggcgcactc ggcgcctggt
     2581 tctccgacct cgcgtatttc gtgttcggct attcggtctg gtgggcggtg gcggtcgcgc
     2641 tgcgcgcctg gctgggcggg ctggcgcgcg tgctgcgcac cgaccgcgct gcgccagcag
     2701 tgccactgca cgcgccgcgg ccggtgtggc tgttctgggt cggcctggcg ttgctgctcg
     2761 cggccagcgc gtcgctcgaa tggacccgcc tgtaccagtg ggaagggcgg gtcgccggcg
     2821 gccacgccgg cggcgcgctc ggctacacgc tgggcagcgc gagccagcgc ctgatcggtt
     2881 tcgccggctc cggcgtgctg tggatcgccg cgctggtggc cggcatcgcc ctggcgctgc
     2941 gcttctcgtg gctgcgactg gccgaggcga tcggtgcctg ggccgagtcg ctgcgcgaac
     3001 ggcgcgcgct gcgcaccgag cgcgccgagg acgcgcgcgt cggcgagcgc gcgctgcgcg
     3061 agcgcgaggt gatcgtcaac gtcgagcacc agctccacga ggaccacctg ccgatcgtga
     3121 tcgaggcgcc ggtgttcgag gtgcccaaga gcgagcgcgt cgtcaaggaa cgccagaagc
     3181 cgctgttcgt cgagctcgcc gacaccaggc tgccgcaggt cgacctgctc gacgcgtcgc
     3241 ccggccgcac cgagaccgtg tcgcccgaga cgctggagat gacctcgcgg ctgatcgaga
     3301 agaagctcaa ggatttcggc gtcgaggtgc gcgtggtcgc ggcctcgccg ggcccggtga
     3361 tcacgcgcta cgagatcgag ccggccaccg gcgtcaaggg tgcgcaggtg ctgaacctgt
     3421 cgaaggacct ggcgcgctcg ctgagcctgg tcagcatccg cgtcgtcgag accatccccg
     3481 gcaagacgac gatggcgctg gagctgccga acccgaagcg ccagaccatc cgcttgtccg
     3541 agatcctcgg ctcgaccgtc tacaacgacg ccagctcgca gctgacgatg gggctgggca
     3601 aggacatcgt cggcaacccg gtcgtcgccg acctcgcgaa gatgccgcac atcct
//
LOCUS       DATMJB010000022         3131 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_233227, whole genome shotgun sequence.
ACCESSION   DATMJB010000022 DATMJB010000000
VERSION     DATMJB010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233227"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VNU48_00475"
     CDS             complement(<1..1264)
                     /locus_tag="VNU48_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWI09783.1"
                     /translation="MSSPETTPSAAAPQGNPARKKALTAVAAAVVVAGIAYGAYWALV
                     LNHFESTDNAYVQGNVVQLTPQVGGTVVAINADDTDHVKAGQLLVRLDPADAQVALDQ
                     AEAQLAQTVREVRTLYANNSTLKAQIALREADLARARSDEARAQDDVARRSPLVATGA
                     VGKEEFNHSTAALAAAKSSVVAAQSALLAAREQLSSNQALTDGIAVEQHPNVLRASAR
                     VREAYLALRRADLLAPVDGYVAKRSVQLGQRVQAGAPLMSVIALNQVWVEANFKESQL
                     RRLRLDQPVTLEADVYGKKVEYRGAIEGLGAGTGAAFALLPAQNATGNWIKVVQRVPV
                     RIKLDPKDLIEHPLRVGLSMDAKVDISKLDGRMLADASRESTTSAQTKVFDQDNTAAD
                     AEVRKIIVANGGRASAIAVASGAATKPPA"
     gene            complement(1308..2807)
                     /locus_tag="VNU48_00480"
     CDS             complement(1308..2807)
                     /locus_tag="VNU48_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWI09784.1"
                     /translation="MTEAAAVRRAVQEHAHRAKTLLPALTAIAVAIASSVVLTLSGCA
                     NSAGIVPVAQAVAPASIGLDPSNPATPAVATDWWRAFGDTTLDALVERALTGSPGLRV
                     AQARLARAQAATAGAQSVDGVHVSGELNATRQRFSATSIYPPPLGGGIFTLGTAQIGA
                     SYEFDFFGRNHAAIEAALGTERAAQAEVQAARVLLASNVARTYVQLGRLFEQREVATR
                     SLRSRDEILALIKQRVQGGLDTSVELRQGEGALPESRQQIEQLDEQIALGRHALAALT
                     AQAPDALDTLVVPLRTVQAVPVPAALPADLLGRRADISAARWRIEAAGSDVKSAKASF
                     YPNINLSAFVGLSSIGLDRLVRSASEQYGVGPALTLPIFDAGRLRANLRGKTADLDAA
                     IESYNAAVIDAVHDVADQISSLRSIERQQLQQVGAQDAAESAYDLAAQRYKAGLGTYL
                     TVLNAEASVLNQRRLAADLKARALDVQIALIRALGGGYVADVQTAAAGQ"
     gene            complement(2889..>3131)
                     /locus_tag="VNU48_00485"
     CDS             complement(2889..>3131)
                     /locus_tag="VNU48_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWI09785.1"
                     /translation="RLLDRLEKKGLCKRVRSTEDRRVVRVEMTPDGEAAIAEVPAVLA
                     EVLNAHLAGFSKTEWHALRNYLQRMLEAGEALREAG"
BASE COUNT          391 a         1124 c         1122 g          494 t
ORIGIN      
        1 cggccggcgg cttcgtcgcg gcgccgctcg ccaccgcgat cgcgctcgcc cggccgccat
       61 tggcgacgat gatcttgcgc acctcggcgt ccgccgcggt gttgtcctga tcgaagacct
      121 tggtctgcgc gctcgtcgtc gactcgcgcg acgcatcggc gagcatgcgg ccgtcgagct
      181 tgctgatgtc gaccttcgcg tccatcgaca ggccgacgcg cagcgggtgc tcgatcagat
      241 ccttcggatc gagcttgatg cgcaccggca cgcgctgcac gaccttgatc cagttgccgg
      301 tcgcgttctg tgccggcagc agcgcgaacg ccgcgccggt gcccgcgccc agcccttcga
      361 tcgcgccgcg gtactcgacc ttcttgccgt acacgtcggc ctcgagcgtg accggctggt
      421 ccaggcgcag gcgccgcaac tggctttcct tgaagttcgc ctcgacccag acctggttca
      481 gcgcgatcac cgacatcagc ggcgcgccgg cctgcacgcg ctggccgagc tgcacgctgc
      541 gcttggccac atagccgtcg accggcgcga gcaggtcggc acgacgcagc gcgaggtagg
      601 cctcgcgcac ccgcgccgac gcacgcagca cgttcgggtg ctgctcgacc gcgatgccgt
      661 cggtcagggc ctggttcgag ctcagctgct cgcgcgccgc gagcagcgcc gattgcgccg
      721 cgaccacgct gctcttggcc gccgcgagcg ccgcggtcga gtggttgaac tcttccttgc
      781 cgaccgcacc ggtggccacc agcggcgagc gccgcgcgac gtcgtcctgc gcgcgcgcct
      841 cgtcgctgcg ggcgcgcgcc aggtcggcct cgcgcagtgc gatctgcgct ttcaacgtgc
      901 tgttgttcgc gtacaaggtg cgcacctcgc gcacggtctg cgcgagctgc gcctcggcct
      961 ggtcgagcgc gacctgcgca tcggccgggt cgagccggac cagcaactgg ccggccttca
     1021 cgtggtcggt gtcgtcggcg ttgatcgcga cgacggtgcc gccgacctgc ggcgtgagtt
     1081 gcacgacatt gccctgcaca taggcgttgt cggtcgactc aaagtggttc agcaccagcg
     1141 cccagtacgc gccgtaggcg atgccggcga cgacgaccgc ggcggccacc gcggtcagcg
     1201 ctttcttgcg ggccgggttg ccttgcggcg cggcggcgga gggagtggtt tcaggggagc
     1261 tcatgcggat tctccggatt cgattcgatg ttgcttcagg cgccggctca ctggccggcc
     1321 gccgcggtct ggacatcggc gacatagccg ccgccgagcg cgcggatcag cgcaatctgc
     1381 acgtcgagcg cgcgcgcctt caggtcggcg gccaggcggc gctggttcag caccgacgcc
     1441 tcggcgttca gcacggtcag gtaggtgccc aggccggcct tgtagcgctg cgccgccaga
     1501 tcgtaggccg attcggccgc gtcctgcgcg ccgacctgtt gcagctgctg gcgctcgatc
     1561 gagcgcagcg agctgatctg gtcggcgacg tcgtgcaccg cgtcgatcac cgcggcgttg
     1621 tagctctcga tcgcggcgtc gaggtcggcg gtcttgccac gcaggttcgc gcgcaggcgg
     1681 ccggcatcga agatcggcag cgtcagcgcc gggccgacgc cgtactgctc gctggccgag
     1741 cgcacgagac ggtccagccc gatgctggac aggccgacga acgcgctcag gttgatgttc
     1801 ggatagaagc tcgccttcgc gctcttcacg tcgctgccgg cggcctcgat gcgccagcgc
     1861 gcggcgctga tgtcggcacg gcggccgagc aggtcggccg gcagcgcggc cggcaccggc
     1921 accgcctgca ccgtgcgcag cggcaccacc agcgtgtcga gcgcgtccgg cgcttgcgcg
     1981 gtcagcgcgg ccagcgcgtg gcgcccgagc gcgatctgtt cgtcgagctg ctcgatctgc
     2041 tggcgcgatt cgggcagcgc gccctcgccc tggcgcagct cgacgctggt gtccaggccg
     2101 ccctgcacgc gctgcttgat cagcgcgagg atctcgtcgc gtgatctgag cgagcgcgtc
     2161 gcgacctcgc gctgctcgaa cagccgcccg agctgcacat aggtgcgcgc gacattgctc
     2221 gcgagcagca cgcgcgcggc ctgcacctcg gcctgcgcgg cgcgctcggt gccgagcgcg
     2281 gcctcgatcg ccgcgtggtt gcggccgaag aaatcgaact cgtaggacgc gccgatctgc
     2341 gcggtgccga gcgtgaagat gccgccaccc agcggcggcg ggtagatgct ggtggcgctg
     2401 aagcgctggc gcgtcgcgtt gagctcgccg ctgacgtgca cgccgtcgac cgactgcgca
     2461 ccggcggtcg cggcctgggc gcgcgccagg cgcgcctgcg cgacgcgcag gcccgggctg
     2521 ccggtcagcg cgcgctcgac cagcgcgtcc agcgtggtgt cgccgaacgc gcgccaccag
     2581 tccgtcgcca cggcgggcgt ggcggggttg ctcgggtcca ggccgatgct ggcgggcgcg
     2641 acggcttggg cgaccggcac gatgccggcg ctgttggcgc agccgctgag ggtcagcacg
     2701 acgctggagg cgatcgccac cgcgatcgcg gtgagcgccg gcagcagcgt cttcgcgcga
     2761 tgcgcgtgct cctgcaccgc acgccgaacg gcggcggctt cggtcaatag ttgttgtggc
     2821 ttcatttgct taggcaatca attcgatgac atttggaccc gcaacgcggc gcggcaccgg
     2881 cgccgccgtc agccggcctc gcgcagcgcc tcgccggctt cgagcatgcg ctgcaggtag
     2941 ttgcgcaacg catgccactc ggtcttcgag aaccccgcca ggtgggcgtt caggacctcg
     3001 gccagcacgg caggcacctc ggcgatcgcg gcctcgccgt cgggcgtcat ttcgacgcgc
     3061 acgacgcgcc ggtcctcggt cgagcgaacg cgcttgcaca ggcccttttt ctccaggcga
     3121 tcgagcagcc g
//
LOCUS       DATMJB010000023         4992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_528823, whole genome shotgun sequence.
ACCESSION   DATMJB010000023 DATMJB010000000
VERSION     DATMJB010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528823"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2171
                     /gene="acnA"
                     /locus_tag="VNU48_00490"
     CDS             <1..2171
                     /gene="acnA"
                     /locus_tag="VNU48_00490"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HWI09786.1"
                     /translation="VGGIEAEAAMLGQPVYFLTPDVVGFEFSGRLREGVTATDLVLAV
                     TEILRREKVVGKFVEFFGAGTESLSVPDRATMANMAPEYGATMGFFPVDDKTIEYFRG
                     TGRTRAEIEAFEAYFKAQGLFGAPRAGAVDYTKVVTLDLGSVAPSLAGPKRPQDRIEL
                     GNVKAQFTSLFSKPPGENGFNQPAAQLHTRHLVRAQDAAEKVPAEAPAPAPGAPRQVV
                     EMASEHPTLEAAQSEAKSLQPKVGDMSLGNGDVLIAAITSCTNTSNPSVLLAAGLLAK
                     KAVEAGLKVAPHIKTSLAPGSRIVTEYLEKAGLLPYLEKLGFSVAAYGCTTCIGNAGD
                     LRPEFNEAITKNDLVCAAVLSGNRNFEARIHPNLKANFLASPPLVVAYAIAGTVLKDV
                     MTEPLGKGKGGRDVYLGDIWPSSDEIHALMKYAMNGKAFKANYDKVASEPGPLWTKIK
                     GAGGQVYNWPASTYIAKPPFFDDFTMAPAHLVAGVRGARAMALFGDSITTDHISPAGS
                     IKEASPAGAWLKANGVAKADFNSYGSRRGNHDVMMRGTFANVRIKNLMIPPGADGSRE
                     EGGVTLFQPSGEKMFIYDAAMKYIAQGTPTVIFAGEEYATGSSRDWAAKGSQLLGVKA
                     VVARSFERIHRSNLVGMGVLPLQFRGSDSWESLGIRGDESFDIQLADEIAPQQEATLV
                     IHRDGVAHKVALTLRIDTPIEVDYYKHGGILPFVLRQLLE"
     gene            complement(2292..2645)
                     /locus_tag="VNU48_00495"
     CDS             complement(2292..2645)
                     /locus_tag="VNU48_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HWI09787.1"
                     /translation="MNAAPTLFGIPNCDTVKKARAWLAANGREVHFHDFKKAGVPEDR
                     LDAWLRAAGWERLLNRKGTSWRQLDEAVRAGVTDAAGAKRVMLAQPSTIKRPVVEWPD
                     GAITVGFDAADWSRR"
     gene            complement(2642..3157)
                     /locus_tag="VNU48_00500"
     CDS             complement(2642..3157)
                     /locus_tag="VNU48_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09788.1"
                     /translation="MFDPRFPGRRAARAAAALTVALSGCATLAPGGLAPGTPIATARQ
                     ALFGPSGEYALPGGGTRLEFRQGSFGRQTFMLDFDKAGLLVSSQQVLTEPNFATIAPG
                     MAEHEVLMRLGHPADAFSVQWQKLHVWNYRYFGGDCVWFQVSISDATRQVTEAGIGPD
                     PACDAPEGKGL"
     gene            3254..4243
                     /locus_tag="VNU48_00505"
     CDS             3254..4243
                     /locus_tag="VNU48_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI09789.1"
                     /translation="MSSATLATMPFVQSLAHGIHVIDTGFHRAVFDASYLIVENGRGA
                     FVDTGTNDSVPRLLAALAAAGLAREAVDYVIATHVHLDHAGGVGLLMQQLPDAQLVVH
                     PLGARHLIDPSRLMNSARSVYGDAEVARTYGHVVGVPAERVLRTADGMTLELAGRRLQ
                     FLDTPGHARHHHCIWDARSRGFFTGDAFGLSYREFDTAKGAWLMPTTTPVQFEPEPLR
                     RSIQRMLAYAPECLYLTHYGRVTEVPRLADLLLTQLDAMVALARSLHDDERRHAALVE
                     GLLAIHLASLRAHGCTLSDARVRDLLALDLELNAQGLAVWLDRRPRVNAGDLA"
     gene            4240..>4992
                     /locus_tag="VNU48_00510"
     CDS             4240..>4992
                     /locus_tag="VNU48_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI09790.1"
                     /translation="MTTAVNFHLEDRVAIVTGAAQGIGAACAERLAQDGAAVALWDVD
                     DVRGQALAAELGAQGRRAIYQHCNVADKGEVDAALAATVQAFGRVDALVNNAGIFRAA
                     DFLDITEADWDAVIDVNLKGAFLVGQAVARAMVAAKVPGTIVNMSSVNGILAIPSIAS
                     YNASKGGVNQLTRVMALALADHGIRVNAVAPGTIATELAQRAVLGSEEAKQRIMSRTP
                     MKRLGEPREIADVCAFLISSASSYITGEIVYID"
BASE COUNT          781 a         1777 c         1714 g          720 t
ORIGIN      
        1 gcgtgggcgg gatcgaggcc gaagcggcga tgctcggcca gccggtctac ttcctgacgc
       61 ccgacgtcgt cggcttcgag ttcagcgggc gcctgcgcga aggcgtgacc gcgaccgacc
      121 tcgtcctcgc cgtgaccgag atcctgcgcc gcgagaaggt cgtcggcaag ttcgtcgagt
      181 tcttcggcgc cggcaccgaa agcctctcgg ttcccgaccg cgcgacgatg gccaacatgg
      241 cgcccgagta cggcgcgacg atggggttct tcccggtcga cgacaagacg atcgagtact
      301 tccgcggcac cggccgcacc agggccgaga tcgaggcctt cgaggcctac ttcaaggcgc
      361 agggcctgtt cggcgcgcca cgcgccggcg cggtcgacta caccaaggtc gtcacgctcg
      421 acctcggcag cgtggcgccg agcctggccg ggccgaagcg cccgcaggac cgcatcgagc
      481 tcggcaacgt gaaggcgcag ttcacctcgc tgttcagcaa gccgcccggc gagaacggtt
      541 tcaaccagcc ggccgcgcag ctgcacacgc gccacctggt gcgcgcgcag gacgccgccg
      601 agaaggtgcc cgccgaggcg cccgcacccg cgcccggcgc gccgcgccag gtggtcgaga
      661 tggccagcga gcacccgacg ctggaggccg cgcagtccga agcgaaatcg ctgcagccca
      721 aggtcggcga catgtcgctg ggcaacggcg acgtgctgat cgcggcgatc acgtcgtgca
      781 ccaacacctc gaacccgagc gtgctgctgg cggccgggct gctcgcgaag aaggcggtcg
      841 aggccgggct gaaggtcgcg ccgcacatca agacctcgct ggcgccgggc tcgcgcatcg
      901 tcaccgaata cctcgagaag gcggggctgc tgccgtacct cgagaagctc ggtttcagcg
      961 tcgccgccta cggctgcacg acctgcatcg gcaatgccgg cgacctgcgg cccgagttca
     1021 acgaagcgat cacgaagaat gacctggtgt gtgccgcggt gctgtcgggc aaccgcaact
     1081 tcgaggcgcg catccacccg aacctgaagg cgaacttcct ggccagcccg ccgctggtgg
     1141 tggcctacgc gatcgccggc accgtgctga aggacgtgat gaccgagccg ctcggcaagg
     1201 gcaagggcgg gcgcgatgtg tacctcggcg acatctggcc gagcagcgac gagatccacg
     1261 cgctgatgaa gtacgcgatg aacggcaagg ccttcaaggc gaactacgac aaggtcgcga
     1321 gcgaacccgg cccgctgtgg accaagatca agggagcggg cggccaggtc tacaactggc
     1381 cggcctcgac ctacatcgcg aagccgccgt tcttcgacga cttcacgatg gcgccggccc
     1441 atctggtggc cggcgtgcgc ggtgcgcgcg cgatggcgct gttcggcgac tcgatcacca
     1501 ccgaccacat ctcgccggcc ggctcgatca aggaggcttc gcccgccggc gcctggctga
     1561 aggcgaacgg cgttgccaag gccgacttca actcctacgg ttcgcgccgc ggcaaccacg
     1621 acgtgatgat gcgcggcacc ttcgcgaacg tgcgcatcaa gaacctgatg atcccgcccg
     1681 gcgccgacgg ctcgcgcgag gaaggcggcg tgacgctgtt ccagccctcg ggcgagaaga
     1741 tgttcatcta cgacgccgcg atgaagtaca tcgcgcaagg cacgccgacc gtgatcttcg
     1801 ccggcgagga atacgccacc ggctcgagcc gcgactgggc cgcgaagggc tcgcagctgc
     1861 tcggcgtgaa ggcggtcgtc gcacgcagct tcgagcggat ccaccgcagc aacctcgtgg
     1921 gcatgggcgt gctgccgctg cagttccgcg gcagcgattc atgggagtcg ctgggcatcc
     1981 gcggcgacga gagcttcgac atccagctcg ccgacgagat cgcgccgcag caggaggcca
     2041 cgctggtgat ccaccgcgac ggcgttgcgc acaaggtggc actgaccttg cgcatcgaca
     2101 cgccgatcga ggtggactac tacaagcacg gcggcatcct gccgttcgtg ctgcggcagt
     2161 tgctggagta atggaacccc cggctgcggg tgtgcagcca cccccagggg ccacttgggg
     2221 ccccttcgtg gcccttgggg tgctcgggcc gcttggggcg gcccggcgct ggcccgagcc
     2281 cgccgcgtcg ctcagcgccg cgaccagtcg gcggcgtcga agccgacggt gatcgcaccg
     2341 tcgggccact cgacgaccgg gcgcttgatc gtgctgggct gcgcgagcat cacgcgcttc
     2401 gcgccggcgg cgtcggtgac gcccgcgcgc acggcctcgt cgagctggcg ccagctcgtg
     2461 cctttgcggt tcaagagccg ctcccagccg gccgcgcgca gccatgcgtc gagtcggtct
     2521 tcgggcacgc cggccttctt gaagtcgtgg aagtgcacct cgcgcccgtt cgccgcgagc
     2581 caggcgcgcg ccttcttcac cgtgtcgcag ttcgggatgc cgaacagcgt gggcgcggcg
     2641 ttcacaagcc tttcccttcg ggtgcgtcgc aggccgggtc gggcccgatg ccggcctcgg
     2701 tgacctgccg tgtcgcatcg ctgatcgaga cctggaacca gacgcagtca ccgccgaagt
     2761 agcggtagtt ccagacgtgc agcttctgcc actgcacgct gaacgcgtcg gccgggtggc
     2821 ccaggcgcat cagcacctcg tgttccgcca tgccgggcgc gatggtcgcg aagttcggct
     2881 cggtcagcac ctgctgcgac gagaccagca ggccggcctt gtcgaaatcg agcatgaagg
     2941 tctgccggcc gaacgagccc tgcctgaact cgagccgagt gccaccgccg ggcaatgcgt
     3001 actcgccgga aggaccgaac agcgcctgcc gcgccgttgc gatcggggtg ccgggcgcca
     3061 ggccgcccgg tgccagcgtg gcgcacccgc tcaacgcgac cgtgagcgcc gctgccgccc
     3121 gcgctgcacg ccggcccgga aaccgtgggt cgaacatcat cgtctccgca tgggtgaatc
     3181 tcctgcattc tgacgccggc cgcgcgcgcg agtttcactc attccggacg cgccggctcc
     3241 atagaatcga cgcatgagtt ccgccaccct cgcgaccatg cccttcgtcc agtcgctggc
     3301 ccacggcatc cacgtcatcg acaccggttt tcaccgagcg gtgttcgacg ccagctacct
     3361 gatcgtcgag aacggccgcg gcgccttcgt cgacaccggc accaacgatt cggtgccgcg
     3421 cctgctggcc gcgctggccg ccgccggcct ggcgcgcgag gcggtcgatt acgtgatcgc
     3481 cacgcacgtg cacctcgacc acgccggcgg tgtcgggctt ctgatgcagc agctgcccga
     3541 tgcgcagctc gtcgtgcacc cgctgggcgc gcggcacctg atcgacccga gccggctgat
     3601 gaacagcgcg cgcagcgtct atggcgacgc cgaggtcgcg cgcacctacg gccacgtggt
     3661 cggcgtgccc gccgagcgcg tgctgcgcac tgccgacggc atgaccctcg agctggccgg
     3721 tcgacgcctg cagttcctcg acacgcccgg ccacgcccgc caccaccatt gcatctggga
     3781 cgcacgcagc cgcggcttct tcaccggcga cgccttcggc ctgtcgtacc gcgagttcga
     3841 caccgcgaag ggtgcctggc tgatgccgac gacgacgccg gtgcagttcg agcccgagcc
     3901 gctgcggcgc tcgatccagc gcatgctggc gtacgcgccc gagtgtctgt acctgacgca
     3961 ctacggccgc gtcaccgagg tgccacgcct ggccgatctc ttgctgaccc agctcgatgc
     4021 gatggtcgcg ctggcccgct cgctgcacga cgacgaacgg cgccacgctg ccctggtcga
     4081 aggcctgctg gcgatccacc tggcgagttt gcgcgcgcac ggctgtacgc tgtccgatgc
     4141 gcgcgtgcgc gacctgctcg cgctcgacct cgagctgaac gcccagggcc tcgcggtgtg
     4201 gctggaccgc cgcccgcgcg tgaacgcagg agacctcgca tgacgacggc cgtgaacttc
     4261 cacctcgaag accgcgtcgc gatcgtgacc ggtgccgcgc aaggcatcgg cgcggcctgc
     4321 gccgagcggc tcgcgcagga cggcgcggcc gtcgcgctgt gggatgtcga cgatgtgcgc
     4381 ggccaggcgc tggccgccga attgggtgcg cagggccggc gcgcgatcta ccagcattgc
     4441 aacgtggccg acaagggcga ggtcgacgcg gcgctcgccg ctacggtgca ggctttcggc
     4501 cgcgtcgatg cgctcgtcaa caacgccggc atcttcaggg ccgccgattt cctcgacatc
     4561 accgaggccg actgggacgc cgtcatcgac gtcaacctga agggcgcgtt cctggtcggc
     4621 caggcggtcg cgcgcgcgat ggtcgcggca aaggtgccgg gcacgatcgt caacatgagc
     4681 tcggtgaacg gcatcctggc gatcccgagc atcgcgagct acaacgccag caagggcggc
     4741 gtgaaccagc tgacgcgggt gatggcgctg gcgctggccg accacggcat ccgcgtcaac
     4801 gcggtcgcgc ccggcacgat cgcgaccgag ctcgcgcagc gcgcggtgct cggcagcgaa
     4861 gaggcgaagc agcgcatcat gagccgcacg ccgatgaagc gcctgggcga gccgcgcgag
     4921 atcgccgacg tctgcgcgtt cctgatcagc agcgcctcga gctacatcac cggcgagatc
     4981 gtctacatcg ac
//
LOCUS       DATMJB010000024         8446 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_173675, whole genome shotgun sequence.
ACCESSION   DATMJB010000024 DATMJB010000000
VERSION     DATMJB010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173675"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /gene="frc"
                     /locus_tag="VNU48_00515"
     CDS             <1..972
                     /gene="frc"
                     /locus_tag="VNU48_00515"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007178896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HWI09791.1"
                     /translation="GALDRMGFTWEYIQSLNPGMILASVKGFSEGHHYEDLKVYENVA
                     QCAGGAASTTGWWKGENSEPTISSAALGDSNTGMHLAIGILTAYIGRQKTGKGQKVAV
                     SMQDAVLNLCRVKMRDQQRLERIGYLEEYPQYPHEMEAFADKVTPRGGNAGGGGQPGW
                     ILKCKGWETDPNAYIYFTVQGHAWEPICDALGKPEWKTDPAYTTPRARQPHIMEIFAT
                     IEDYIKDKTKYEAVDIFRKFDIPCAPVLSMKELLVDESLRKSGSIVEVPHKARGSYFT
                     IGSPIKFSDLKPEVTASPLLGEHTDDVLAELGYSAQQIAAMHEVKAV"
     gene            973..1422
                     /locus_tag="VNU48_00520"
     CDS             973..1422
                     /locus_tag="VNU48_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWI09792.1"
                     /translation="MTELSHASVDLEQLVAGAGDAIMVCDAAGAITLWNRAAERIFGF
                     GEAEALGRSLDLIIPERQRGRHWEGYHKTMATAVTKYGADVLRVPALHKDGHTVSIAF
                     TVSMLFSSDRQVTGIVAVVRDETTRFADERKLRARLAEAEAQIKGAS"
     gene            1422..2669
                     /gene="frc"
                     /locus_tag="VNU48_00525"
     CDS             1422..2669
                     /gene="frc"
                     /locus_tag="VNU48_00525"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HWI09793.1"
                     /translation="MSKALEGVRILDFTHVQSGPTCTQLLAWFGADVIKVERAGEGDA
                     TRGQLRDIPGVDSLYFTMLNHNKRSITLDTKNPKGRQVLDTLIKTCDVLVENFAPGAL
                     DRMGITWEHIQKLNPRMVVASVKGFGPGKYEDCKVYENVAQCAGGAASTTGFDDGPPM
                     VTGAQIGDSGTGLHLALGIVAALYQRNTTGRGQKVLAPMQDAVLNLCRVKLRDQQRLE
                     RTHTMHEYPQYPDGQFGDAVPRAGNASGGGQPGSILKCKGWETDPNAYIYFITQSGVW
                     PSVCKVIGEEDWIDDEAFATPAARLLHLKPIFARIEKWTMTKTKFEAMEILNQYDIPC
                     GPILSMKEIAEDESLRQTGTIVEVDHPTRGKYLTVGNPIKMSDSPTEVTRSPLLGEHT
                     DEVLRQLGYSSDDVAMLRAERVI"
     gene            2835..3164
                     /locus_tag="VNU48_00530"
     CDS             2835..3164
                     /locus_tag="VNU48_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09794.1"
                     /translation="MFNDLPIEYRDQELVRNAYNAAFHELGLRWYWDADTYERLTRCS
                     GTPQEQLRHYLESQQPHLLKAYDAEFLIGIVLEKQSLHCERAAQRGPMRCRPFDWSQT
                     LGGELGC"
     gene            3199..4008
                     /locus_tag="VNU48_00535"
     CDS             3199..4008
                     /locus_tag="VNU48_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWI09795.1"
                     /translation="MQRHWVRYEHEGHAGFGTLEGARVQVHAGDMFGDCAPIDAVLAL
                     DEIQLLTPTVPTKVIALWNNFRALGDKLGLAEPAEPLYLLKPPNSFLASGETIAQPVQ
                     DGKVVFEGELGIVIGRACTAVEAAQALDHVFGYTCANDVTLADVLNRDPSFAQWTRAK
                     GFDTFCPMGPVVATGLDASMLTVVTRLNGEERQRYPISDMRFSVAQLVSRISKDMTLH
                     PGDVILCGTSLGVGSMKPGSSVEVEIDGIGVLANRYGERAPPNEFNSGRPA"
     gene            4005..5015
                     /locus_tag="VNU48_00540"
     CDS             4005..5015
                     /locus_tag="VNU48_00540"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWI09796.1"
                     /translation="MKIAVIAAGAIGGLVGAKLALAGNDVSFLVRGANLEAIRRDGIT
                     LIAADGARQVVRNVTATDDYRATGAQDIVILAMKAHQVDAVAEQVPLLFGPDTVVVTM
                     QNGIPYWYFHKHGGPLEGTTVRSVDPDGQAIRAIPVDRVIGCVVYPASELVAPGVVKH
                     IEGDRFPLGELDGSSSERVRRVSECFAAAGFKAPVLDNIRAEIWLKLWGNLSFNPISG
                     LSHSTLLDICEYPPSRALAAAMMTEAQTVAHKLGIEFRVSLEKRIAGAEKVGKHKTSM
                     LQDVEAGRAPEIDALVGAVVELARLTETPTPHISTVYALVKLLARTMHAQRGQVRMCE
                     VA"
     gene            5052..5990
                     /locus_tag="VNU48_00545"
     CDS             5052..5990
                     /locus_tag="VNU48_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWI09797.1"
                     /translation="MKHATLRQLKVFEAVARLLSFSRAAEELHLTQPAVSTQVGKLEE
                     HAGNALFEQFGKKIYLTPAGVELLQISRAIIQQFEAAENAMAHFKGVAGGRLNVGVIS
                     AGDYFFPHLLVAFASRHQGVTLNFTVHNRAELLTHIVDNLTDLAIMVRPPTDLETINQ
                     PFAPHPYVIVAAPGHPLVGHRNIPRAMLKREPFVVREQGSDTWHSMEDGFGGDLADVN
                     VAMEICSTETIKQAVIAGMGISFLSAHTISHELRDGELCVLDVEGFPLMLNWYIVHRR
                     TKRLAPVAQAFKDFLLCEGAALIERVVPYRTLARAA"
     gene            complement(6027..6212)
                     /locus_tag="VNU48_00550"
     CDS             complement(6027..6212)
                     /locus_tag="VNU48_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09798.1"
                     /translation="MSHNLLNSKPQPINDASPTSRAVREFVAAMPIVMLVTAVIGAFR
                     SRPAPARATTSRQFSTR"
     gene            6438..7601
                     /locus_tag="VNU48_00555"
     CDS             6438..7601
                     /locus_tag="VNU48_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWI09799.1"
                     /translation="MSGPLKGVTVIELAGIGPGPFAGMMLADMGADVIRVDRIPSPRG
                     ASALEAALRNDSIVDRGRRSIALDMKNARGIETVLALVARADILIEGFRPGVTERLGV
                     GPEACRARNPRLVYGRMTGWGQHGPLAQAAGHDLNYIALSGALHAIGPKDRPPAPPLN
                     LVGDYGGGGMLLALGVVAALFEARQSGRGQVVDAAMTDGAALLMAPLYGMLAKGSWRD
                     ERESNFIDGAAPFYGSFECADARYVAIGPIEPQFYRELLDKCAIDDPQFAQQWERADW
                     PALKAKLAALFKTRTRDAWCALLEGTDACFAPVLSMREAPLHAHNVARATFVESAGVT
                     QPAPAPRFARTPSELPAAAPPIGAHSRELLRTLGHTDAQIDELLRSGVVFATG"
     gene            complement(7645..8133)
                     /locus_tag="VNU48_00560"
     CDS             complement(7645..8133)
                     /locus_tag="VNU48_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015002954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI09800.1"
                     /translation="MTIQKISPFLWYAKEAEQAAAFYTGIFPDSRVVRVTTLPSASPS
                     GPPGSVKIVDFVLFGQPFVAMSAGPLDPFNHAVSFVVNCDDQAELDRYWNALLEGGSP
                     EQCGWLKDRFGLSWQIVPTALAKMMADPDRTKAKRAADAMLGMVKLDIAALQAAHAGT
                     NR"
BASE COUNT         1480 a         2798 c         2882 g         1286 t
ORIGIN      
        1 ggcgcgctcg accgcatggg cttcacctgg gagtacatcc agtcgctcaa ccccggcatg
       61 atcctggcat cggtcaaggg cttcagcgag gggcaccact acgaggacct gaaggtctac
      121 gaaaacgtcg cccaatgtgc cggcggcgcg gcgtcgacca ccggttggtg gaagggcgag
      181 aactcggagc cgaccatctc ctccgccgcg ctcggcgact ccaacaccgg catgcacctg
      241 gcgatcggaa tcctgacggc ctacatcggc cgccagaaga ccggcaaggg ccagaaggtc
      301 gcggtgtcga tgcaggacgc ggtgctcaat ctgtgccgcg tgaagatgcg cgaccagcag
      361 cgcctggagc gcatcggcta cctcgaggag tacccgcagt atccgcacga gatggaagcg
      421 ttcgccgaca aggtgacgcc gcgtggcggc aatgccggcg gtggtggcca gccgggctgg
      481 atcctgaagt gcaagggctg ggagaccgac ccgaacgcct acatctactt caccgtccag
      541 ggccacgcct gggagccgat ctgcgacgcc ctcggcaagc ccgagtggaa gaccgacccg
      601 gcctacacga cgccgcgggc gcgtcagccg cacatcatgg agatcttcgc gaccatcgag
      661 gactacatca aggacaagac gaagtacgaa gccgtcgaca tcttccgcaa gttcgacatt
      721 ccctgcgcgc cggtgctgtc gatgaaggaa ctgctggtcg acgaatcgct gcgcaagagc
      781 ggctcgatcg tcgaggtgcc acacaaggcg cgcggcagct acttcacgat cggcagcccg
      841 atcaagttct ccgacctgaa gcccgaggtg acggcgtcgc cgctgctcgg cgagcacacc
      901 gacgacgtgc tggccgaact cggctattcg gcgcagcaga tcgcggcgat gcacgaggtc
      961 aaggcggtct agatgactga gctctcccac gcgtcggtcg acctggaaca gctcgtcgcc
     1021 ggggccggcg acgcgatcat ggtctgcgac gccgcgggcg cgatcacgct gtggaaccgc
     1081 gccgccgagc gcatcttcgg cttcggtgaa gccgaggcgc tcggccgctc gctcgacctg
     1141 atcatccccg agcgccagcg cggccggcac tgggagggct atcacaagac gatggcgacg
     1201 gcggtcacca aatacggcgc cgacgtgctg cgcgtgccgg cgctgcacaa ggacgggcac
     1261 acggtatcga tcgccttcac cgtctcgatg ctcttttcgt cggaccgcca agtcacgggc
     1321 atcgtcgccg tcgtgcgcga cgaaaccacc cgcttcgccg acgagcgcaa actgcgcgcg
     1381 cgtctggcgg aagcggaggc acagataaaa ggagcgagtt gatgagcaag gccctggaag
     1441 gcgtccggat cctcgatttc acccacgtgc agtcgggccc cacctgcacc cagctgctgg
     1501 cctggttcgg cgccgacgtg atcaaggtcg aacgcgccgg cgagggcgac gcgacgcgcg
     1561 gccagttgcg cgacatcccc ggtgtggaca gcctgtactt cacgatgctc aaccacaaca
     1621 agcgctcgat cacgctggac acgaagaatc ccaagggcag gcaggttctg gacacgctga
     1681 tcaagacctg cgacgtgctg gtcgagaact tcgcgcccgg cgcgctcgac cgcatgggca
     1741 tcacctggga gcacatccag aagctcaatc cgcgcatggt cgtggcctcg gtcaagggct
     1801 tcggccccgg caagtacgag gactgcaagg tctacgagaa cgtcgcgcaa tgcgccggcg
     1861 gcgccgcctc gacgaccggc ttcgacgacg ggccgccgat ggtcaccggc gcgcagatcg
     1921 gcgacagcgg caccgggctg cacctggcgc tgggcatcgt cgccgcgctc taccagcgca
     1981 acaccaccgg gcgcggtcag aaggtgctgg cgccgatgca ggacgcggtg ttgaacctgt
     2041 gccgcgtcaa gctgcgcgat cagcagcgcc tggagcgcac ccacacgatg cacgagtacc
     2101 cgcaataccc ggacggccaa ttcggcgacg cggtgccgcg cgcgggcaat gcgtcgggcg
     2161 gcggacagcc cggttcgatc ctgaagtgca agggctggga gaccgacccg aacgcctaca
     2221 tctacttcat cacgcagtcc ggcgtctggc cctcggtgtg caaggtgatc ggcgaggaag
     2281 actggatcga cgatgaggcc ttcgcgacgc cggcggcgcg cctgctgcac ctgaagccga
     2341 tcttcgcgcg catcgagaag tggaccatga ccaagaccaa gttcgaggcc atggaaatcc
     2401 tgaaccagta cgacattccg tgcggcccga ttctgtcgat gaaggagatc gcggaggacg
     2461 aatccttgcg ccagaccggc acgatcgtcg aggtcgacca cccgacgcgc ggcaagtacc
     2521 tgacggtggg caacccgatc aagatgtccg acagcccgac cgaggtcacg cgttcgccgc
     2581 tgctgggcga gcacacggac gaggtgctca ggcagctcgg ctacagcagc gacgacgtcg
     2641 cgatgctgcg tgcggaacgc gtgatctgag ccgcggcggt cgcgtctcga accgcctcga
     2701 aacataagcc ctcgaagatg gtttcgataa attagtttga ctgtcgctga tcgaagcagg
     2761 agcctacatt ttgaacaagc cgacgcgaga tgcagcgatg cgaaacgcgg cgattccggt
     2821 tcaaggagaa gctcatgttc aacgatctgc cgattgagta ccgcgaccag gaactggtcc
     2881 gcaatgccta caacgcagcc ttccacgagt tgggcctgcg ctggtactgg gacgccgaca
     2941 cctacgagcg tctgacgcgt tgcagcggca cgccgcaaga gcaactgcgg cactacctcg
     3001 aatcgcagca gccgcatctg ctgaaggcct acgacgccga gttcctgatc ggcatcgtgc
     3061 tggagaagca gtcgctgcac tgcgagcgtg ccgcacagcg cgggccgatg cggtgccggc
     3121 ccttcgattg gtcgcagacg ctgggtggcg aactcggctg ctgagatcgt tcacgacgca
     3181 cacaagggaa tcatcaagat gcagcgccac tgggttcggt atgaacatga aggccacgcc
     3241 ggcttcggca cgctcgaagg cgcccgcgtg caggtgcacg ccggtgacat gttcggtgac
     3301 tgcgcgccta tcgatgcggt gctcgcgctc gacgagatcc agctgctgac gccgaccgtg
     3361 ccgaccaagg tgatcgcgct gtggaacaac ttccgcgcgc tcggcgacaa gctcggcctg
     3421 gccgagccgg cggagccgct gtacctgctg aagccgccga attcgtttct cgccagcggc
     3481 gagacgatcg cccagccggt gcaggacggc aaggtcgtct tcgaaggcga actgggcatc
     3541 gtgatcggcc gtgcttgcac cgcggtcgaa gccgcgcagg cgctggacca cgtgttcggc
     3601 tacacctgcg cgaacgacgt gacgctggcc gacgtgctga accgcgaccc gtcgttcgcg
     3661 cagtggaccc gcgcgaaggg cttcgacacg ttctgcccga tgggcccggt cgtcgcgacc
     3721 ggtctggatg cgtcgatgct gaccgtcgtc acgcggctga acggcgaaga gcggcagcgt
     3781 tatccgatca gcgacatgcg cttcagcgtt gcgcaactcg tcagccggat ctcaaaggac
     3841 atgaccctgc atcccggcga cgtgatcctg tgcggcacct cgctcggcgt cggctcgatg
     3901 aagccgggca gcagcgtcga ggtcgagatc gacggcatcg gcgtgctcgc caaccgctac
     3961 ggcgaacgcg cgccgcccaa cgaattcaac tccggaagac ccgcatgaaa atcgcagtga
     4021 tagcagccgg cgcgatcggc ggcctggtgg gtgccaagct cgcgctcgcc ggcaacgacg
     4081 tgagctttct cgtccgcggt gcgaacctcg aggcgatccg ccgtgacggc atcacgctga
     4141 tcgccgccga cggcgcgcgg caggtagtgc gcaacgtcac cgccaccgac gactaccggg
     4201 ccaccggagc gcaagacatc gtcatcctcg cgatgaaggc gcaccaggtc gacgcggtgg
     4261 cggaacaggt gcccctgctg ttcggccccg acacggtggt cgtgacgatg cagaacggca
     4321 tcccgtactg gtacttccac aagcacggcg ggccgctcga agggaccacg gtgcgcagcg
     4381 tcgatcccga cggccaggcg atccgtgcga tccccgtgga tcgcgtgatc ggctgcgtcg
     4441 tgtacccggc gtccgaactg gtcgcgccag gggtcgtgaa gcacatcgaa ggcgaccgct
     4501 tcccgctcgg cgaactcgac ggctcatcga gcgaacgcgt gcggcgcgtg tccgagtgtt
     4561 tcgccgcggc gggcttcaag gccccggtgc tcgacaacat ccgtgccgag atctggctca
     4621 agctctgggg caacctgagc ttcaacccga tcagcggcct gtctcattcc acgctcttgg
     4681 acatctgcga atacccgccg tcgcgcgcgc tggccgccgc gatgatgacc gaggcgcaga
     4741 cggtcgcgca caagctcggc atcgagtttc gcgtgtcgct ggagaaacgc atcgccggcg
     4801 ccgagaaggt cggcaagcac aagacctcga tgctgcagga cgtggaagcc ggccgcgcgc
     4861 ccgagatcga tgcactcgtc ggcgcggtcg tcgagctcgc ccggctgacc gagacgccga
     4921 cgccgcacat cagcaccgtc tatgcgctgg tgaagctgct ggcgcgcacg atgcacgcac
     4981 agcgcggtca ggtgcggatg tgcgaggtgg cctgacgcgc aatcgtgcgc gtgcctgcgg
     5041 acatcgagac gatgaagcac gccacgctgc ggcagctgaa ggtgttcgag gcggtcgcgc
     5101 ggctgctgag tttttcgcgc gccgccgagg agctgcacct cacgcagccg gccgtctcga
     5161 cccaggtcgg caagctggag gagcacgccg gcaacgcgct gttcgagcag ttcggcaaga
     5221 agatctacct gacgccggcc ggcgtcgaac tgctgcagat cagccgcgcc atcatccagc
     5281 agttcgaggc cgccgagaat gcgatggcgc atttcaaggg cgtcgcgggc ggcaggctca
     5341 acgtcggcgt gatcagtgcc ggcgactact tctttccgca cctgctggtg gcgttcgcga
     5401 gccgtcacca gggcgtcacg ctgaacttca cggtgcacaa ccgcgccgag ttgctgaccc
     5461 acatcgtcga caacctgacc gacctcgcga tcatggtgcg accgccgacc gatctggaga
     5521 cgatcaacca gcccttcgcg ccgcatccct acgtgatcgt cgcggcgccg ggtcatccgt
     5581 tggtcgggca tcgcaacatt cccagggcca tgctgaagcg cgagcccttc gtcgtgcgcg
     5641 agcagggctc ggacacctgg cattccatgg aggacggctt cggcggcgat ctggccgatg
     5701 tcaacgtcgc gatggagatc tgcagcaccg agaccatcaa gcaggcggtg atcgccggca
     5761 tgggcatcag cttcctgtcg gcgcacacga tcagccacga gcttcgcgac ggcgagctgt
     5821 gcgtgctcga cgtcgagggc ttcccgctga tgctgaactg gtacatcgtg caccggcgca
     5881 ccaagcgcct ggcgccggtg gcgcaggcgt tcaaggactt cctgctgtgc gagggcgcgg
     5941 cgctgatcga gcgcgtcgtc ccgtatcgca cgctggcccg ggcagcctag cggcactcgc
     6001 gcgccgcggc cgaccgggtc ggtggctcag cgggtcgaga actggcgcga cgtggtggcg
     6061 cgcgcggggg cgggccgcga cctgaaggcc ccgatcaccg cggtgacgag catcacgatg
     6121 ggcatcgccg cgacgaattc gcgcacggcc cggctcgtcg gcgatgcgtc gttgatcggt
     6181 tgcggcttgg aattcaggag gttgtggctc acgatgtttt ctcgaaaggt ttggaagatc
     6241 gactggaggc gattataggg gttaaccctg tgactgcggt caatgtcctg ttgccccgaa
     6301 ggcgattgcc ggcgcgacgc gcgtccggcc tgtctgcttg agcaccgtgc cgactcccgc
     6361 agctactatc cggccacgca ttgggcgtgc agtttcggcg ccgccgacac gtagcagaca
     6421 ccgcggagtg ccgacacatg agtggacccc tgaagggcgt gacggtcatc gaactggcgg
     6481 gcatcgggcc cgggccgttc gccgggatga tgctggccga catgggcgcc gacgtgatcc
     6541 gcgtcgaccg catcccgagc ccgcgcggcg ccagcgcgct cgaggcggcc ctgcgcaacg
     6601 acagcatcgt cgaccgcggg cggcgctcga tcgcgctcga catgaagaac gcgcgcggca
     6661 tcgagaccgt gctcgcgctg gtcgcgcgcg ccgacatcct gatcgagggc tttcgaccgg
     6721 gggtgaccga gcgtctcggc gtcggccccg aagcctgccg cgcgcgcaac ccgaggctgg
     6781 tctacggtcg catgaccggc tggggccagc acggcccgct ggcgcaggcg gccgggcacg
     6841 acctgaacta catcgcgctg tcgggcgcgc tgcacgcgat cgggccgaag gaccggccgc
     6901 cggcgccgcc gctgaacctg gtcggcgact acggcggcgg cggcatgctg ctcgcgctcg
     6961 gcgtcgtcgc cgcgctgttc gaggcgcgcc agtcggggcg gggccaggtc gtcgacgcgg
     7021 cgatgaccga cggcgcggcg ctgctgatgg cgccgctgta cggcatgctg gccaagggct
     7081 cgtggcgcga tgagcgcgag agcaacttca tcgacggcgc ggcgccgttc tacggcagct
     7141 tcgaatgcgc cgacgccagg tacgtcgcga tcggcccgat cgagccgcag ttctaccgtg
     7201 agttgctcga caagtgcgcg atcgacgacc cgcagttcgc gcagcagtgg gaacgcgctg
     7261 actggccggc gctcaaggcc aagctcgccg cgctgttcaa gacacgcacg cgcgacgcct
     7321 ggtgcgcgct gctggaaggc accgacgcct gcttcgcgcc ggtgctgtcg atgcgcgaag
     7381 cgccgctgca tgcgcacaac gtggcgcgcg cgaccttcgt cgaatcggcc ggcgtgacgc
     7441 agcccgcgcc cgcgccgcgc ttcgcgcgca cgccgagcga actgccggcg gccgcgccgc
     7501 cgatcggcgc acacagccgc gagctgctgc gtacccttgg ccacactgac gcgcagatcg
     7561 acgagctgct gcgcagtggc gtggtcttcg cgacaggctg agacgcgcag cggcagcgcg
     7621 tgccgggcga ttctccgagg gcagtcagcg gttcgtgccg gcgtgggccg cctgcaatgc
     7681 ggcgatgtcg agcttgacca tcccgagcat cgcgtcggcg gcccgcttgg ccttggttcg
     7741 atccgggtcg gccatcatct tcgcgagagc ggtcggcacg atctgccacg acaggccgaa
     7801 gcggtccttc agccagccgc attgctctgg cgatccgccc tcgagcagcg cgttccagta
     7861 gcggtcgagc tcggcctgat cgtcacagtt cacgacgaag gatacggcgt ggttgaacgg
     7921 gtcgagcggc ccggcgctca tcgcgacgaa gggctggccg aacagcacga agtcgacgat
     7981 cttcaccgag cccggcggac cgctgggcga cgcgctgggc agcgtggtga cccggaccac
     8041 gcgagagtcc ggaaagatgc cggtgtagaa cgcggcggcc tgttcggcct ccttcgcgta
     8101 ccagaggaag gggctgattt tctggatcgt catcgcagga gtctcctcga ggtgttgtgc
     8161 ggcgtcgaag ccgcgggtcg gtatggacac gacgaagaag gcgggccggg atcgacacgc
     8221 cgacaaccat cggacaagaa gtgtcgtctt ggtttcgctt tgtcgcaggc cgcggtcgcg
     8281 tgacatccgc acagccgcgc cgaatcggcg gctggcacat tgaatcgcgt ccaccgattc
     8341 atcgcgaggc agcccatgaa gatccgccgt gtcctgttga gtctggccag cgtgtggctg
     8401 gtgtccctgg ccatgcccgc cgcggcgcag acgatgcaag aggccg
//
LOCUS       DATMJB010000025         2160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_587713, whole genome shotgun sequence.
ACCESSION   DATMJB010000025 DATMJB010000000
VERSION     DATMJB010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587713"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VNU48_00565"
     CDS             complement(<1..748)
                     /locus_tag="VNU48_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI09801.1"
                     /translation="MRPWSTWGRSWGGQGYCIEACRQTPLLACLMLARLQRFISISLL
                     LGATGWAIGFAAAGRPAWAAVGALAILFGYALFLAAEFVTLYFVQRADVAPRPSVCQL
                     VTAWRHEALTAPRVFLWRQPFRSGAEPDHLPSAARATRRGVVLVHGFVCNRGLWNPWM
                     RRLRARGVPFVAVNLEPVFGSIDHYPQTIEDAVTRIELATGMPVVLVGHSMGGVAIRA
                     WLARFDADARVHRVLTIGSPHHGTWLARFGH"
     gene            730..1458
                     /locus_tag="VNU48_00570"
     CDS             730..1458
                     /locus_tag="VNU48_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09802.1"
                     /translation="MSTTAATILAHLKTVDAERAKRAEVPGLSDRVVAVKAYQQRRFS
                     HTYADLLGSARYGPASRFFLDELYGPGDFTSRDTQFARVVPALVRLFPGEIVDTVATL
                     AELHALSEILDTVMGNQIADEFITGIDYIGAWQRTDRSAQRQRQIDLTLEVASRLDRF
                     TRRALLRNSLRLMRAPARAAGLTELQRFLEAGFDTFREMHGSREFISIVEARERVLAS
                     ELFGADVSDASSESVQRALRGLPT"
     gene            1455..>2160
                     /locus_tag="VNU48_00575"
     CDS             1455..>2160
                     /locus_tag="VNU48_00575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI09803.1"
                     /translation="MNKIFRAAGLTVLERGWLSSNNVLFDDGGRHESVLIDSGYCTHA
                     QQTVALVRNALGTRRLDRVVNTHLHSDHCGGNHALQAVFDCAVDVPAGEVDKVDRWDE
                     SLLTYRDTGQQCPRFNRTGAIGTGDRIRCGAWLWEVVASPGHDPESMVLYQAELRVLI
                     SADALWENGFGVVFPELEGLQAFQDVRATLNRLGQLQVEWVIPGHGAPFQGLDVALNR
                     AHRRLDGFIADPARHAR"
BASE COUNT          376 a          710 c          730 g          344 t
ORIGIN      
        1 tgtgcccgaa gcgcgccagc caggtgccgt ggtgcgggct gccgatcgtc agcacgcggt
       61 gcactcgcgc atccgcgtcg aagcgggcga gccaggcgcg gatcgcgacg ccacccatgc
      121 tgtggccgac gagaacgacc ggcatgccgg tcgcgagttc gatgcgggtc acggcgtctt
      181 cgatggtctg ggggtagtga tcgatcgagc cgaacaccgg ctccagattc accgcgacga
      241 agggcacgcc gcgcgcacgc agccgccgca tccacgggtt ccacagcccg cggttgcaaa
      301 cgaacccatg caccagcacg acaccacggc gcgtcgcgcg agccgcagag ggcaggtggt
      361 ccggttcagc gcccgagcgg aacggctgtc gccacaggaa gacgcgcggc gcagtgagtg
      421 cttcgtgccg ccaggccgtc accagctggc acacggacgg ccggggagcg acgtccgcgc
      481 gctgcacgaa atagagcgtg acgaactcgg ccgcgagaaa gagcgcgtag ccgaacagga
      541 tcgccaaggc gcccaccgca gcccaggccg gccggccggc cgcggcaaag ccgatggccc
      601 agccggttgc gccgagaagc agcgatatcg agatgaacct ctgcagccga gcgagcatca
      661 ggcatgctag cagcggcgtt tgccggcagg cctcgataca gtagccttgc ccgccccagc
      721 tccgacccca tgtcgaccac ggccgcaacc atcctcgcgc acttgaagac cgtcgacgcc
      781 gaacgcgcga aacgtgcgga ggtccccggc ctgagcgacc gtgtcgttgc cgtgaaggcc
      841 taccagcagc gccgcttttc gcacacctat gccgacctgc tgggcagtgc acgctacggt
      901 ccggcgtcgc gcttcttcct cgatgaactc tacggtccgg gggatttcac aagtcgcgac
      961 acgcaattcg cacgggtggt gccggcgctg gtgcggctgt tcccgggcga gatcgtcgac
     1021 acggtggcca cgctggccga actgcatgcg ttgtcggaga tactcgatac ggtgatggga
     1081 aatcagatcg cggatgagtt catcacaggg atcgactaca tcggcgcgtg gcagcgcacc
     1141 gatcgctccg cgcagcggca gcgccagatc gacctgacac tcgaagtcgc gagccgcctc
     1201 gatcggttca cgcgccgggc gctgctgcgc aacagtctgc gcctgatgcg cgcgccggcg
     1261 cgagccgcag gattgaccga actgcagcga ttcctcgaag ccggcttcga cacgtttcgg
     1321 gagatgcacg gctcacggga gttcatctcc attgtcgagg cgcgtgagcg ggtgttggct
     1381 tcggaactat tcggcgctga tgtttcggat gcgagttcgg agtcggtgca gcgcgccctt
     1441 cggggcctgc caacatgaac aagatcttca gggccgctgg actgaccgtc ctcgagcgcg
     1501 gctggctctc gtcgaacaat gtcctgttcg acgacggcgg gcgccacgaa tcggtcctga
     1561 tcgactcggg gtactgcacg catgcgcagc aaacggtggc gctggtgcgc aacgcgctgg
     1621 gtactcgtcg gcttgaccgc gtcgtgaaca cccacttgca ttcagaccac tgcggcggca
     1681 atcacgccct gcaagccgtg ttcgattgcg ccgtcgacgt acctgccggc gaagtcgaca
     1741 aggtggaccg ctgggacgaa tcgctgctga cctaccgcga cacgggtcag cagtgcccgc
     1801 gcttcaaccg cacaggagcc atcggcaccg gcgatcgaat tcgctgcggc gcctggctct
     1861 gggaggtcgt tgcatcaccc ggccacgatc cggaatcgat ggtcctgtac caagccgagt
     1921 tgcgggtgtt gatttcggcc gatgcgctct gggagaacgg tttcggcgtt gtctttccgg
     1981 aacttgaagg gctgcaggcg tttcaggacg tgcgcgccac gctgaatcgc ttggggcagc
     2041 tgcaggtcga atgggtcatc cccggacacg gcgcgccgtt tcaaggcctg gacgttgcgc
     2101 tgaaccgggc tcatcggcgg ctggacgggt tcatcgccga cccggcgcgg catgcccgac
//
LOCUS       DATMJB010000026         2792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_826346, whole genome shotgun sequence.
ACCESSION   DATMJB010000026 DATMJB010000000
VERSION     DATMJB010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826346"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VNU48_00580"
     CDS             <1..658
                     /locus_tag="VNU48_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWI09804.1"
                     /translation="VQISHRYGMILFIASEVMFFVAWFWAFFNSALFPADAVHATRDA
                     VFGCGAGTAMGACAVPGTWPPKSIETFDPWHLPLLNTLLLLTSGTTVTWAHHALLEGD
                     RKGLKYGLILTCILGALFTCVQAYEYGHAAFSFAGNIYGATFFMATGFHGFHVLVGTV
                     FLLVCLFRAYAGHFTPTQHLGFEFAAWYWHFVDVVWLFLFICIYVWFRGAEAVGHAAH
                     "
     gene            727..1116
                     /locus_tag="VNU48_00585"
     CDS             727..1116
                     /locus_tag="VNU48_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HWI09805.1"
                     /translation="MSDDAHPTVTESALRGLACKCPRCGKGKLYGGFLTLRPKCEACG
                     LDYAFIDTGDGPAVFLIMIAGAVVVGSALIVEIKYQPPFWLHAALWLPLILATTLLPM
                     RAMKSLLIALQFHHKAAEGRLIDRDPK"
     gene            1113..1880
                     /locus_tag="VNU48_00590"
     CDS             1113..1880
                     /locus_tag="VNU48_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HWI09806.1"
                     /translation="MSAVPAPRRGVAGFAIVSLMMVSLCIGLGLWQLQRRAEKHVLIA
                     QLAERLVASPSPLPAPSQWSALTRARDEFRRVSFEAIYAPLPDAMVYSAGSGVRDDIS
                     GPGTWAFIPAQLASGETVAINTGFVQNTMQDRAQQDRAVAWFVTGDPVQLTGYIRFPE
                     PPGSLTPAENLGKRLWFTRDHQAMARALGWGERGKPLAPFYIDLEQPVPASGIPKPGA
                     LSVHLKDDHLQYAITWFGLAGAVVIAFGVWLRGQRRS"
     gene            2027..2386
                     /locus_tag="VNU48_00595"
     CDS             2027..2386
                     /locus_tag="VNU48_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWI09807.1"
                     /translation="MSNVAGDFDPIAIVVDWIDACRMRNLDALLDLYADDAKLECACE
                     GSKHEGRVALASYWRPRLEQVAPTAFGLEQVTPAADGVVLDYISFEGKHVRAFFAFDA
                     AGRIQQTRCGPGPALQT"
BASE COUNT          435 a          947 c          851 g          559 t
ORIGIN      
        1 ggtgcagatc agccaccgct acgggatgat cctgttcatc gcctccgaag tgatgttctt
       61 cgtcgcctgg ttctgggcct tcttcaactc ggcgctgttc ccggccgatg cggtgcatgc
      121 cacccgcgac gccgtcttcg gctgcggcgc cggaaccgcg atgggcgcct gcgcggtgcc
      181 cggcacctgg ccgccaaaga gcatcgagac cttcgatccc tggcatctgc cgctgctcaa
      241 cacgctgctg ctgctgacct cgggcaccac cgtcacctgg gcgcatcacg cgctgctgga
      301 aggcgaccgc aaggggctga aatacggcct gatcctgacc tgcattctcg gcgcgctctt
      361 cacctgcgtg caggcctatg aatacggcca tgccgcgttc tcgttcgccg gcaacatcta
      421 cggcgcgacc ttcttcatgg cgaccggctt ccatggtttc cacgtgctgg tcggcaccgt
      481 gttcctgctg gtctgcctgt tccgcgctta cgccgggcac ttcaccccga cccagcatct
      541 cggcttcgaa tttgccgcct ggtactggca cttcgtcgac gtggtgtggt tgttcctgtt
      601 catctgcatc tatgtctggt tccgcggcgc tgaagccgtc ggacacgccg cgcattgacc
      661 tgacatcatt gtgttacaag ggggcggtcg tgcgaccgcc cctttttcat tttaccgagc
      721 tcaccgatgt ctgatgacgc ccaccccacg gtgaccgaga gcgcgctgcg cggtctcgcc
      781 tgcaaatgtc cgcgttgcgg caagggcaag ctctatggcg gcttcctgac gctgcggccc
      841 aaatgcgagg cctgcgggct cgactatgct ttcatcgaca ccggcgacgg ccccgcggta
      901 ttcctgatca tgatcgccgg cgccgtcgtc gtcggctccg cgctgatcgt cgagataaaa
      961 tatcagccgc cgttctggct gcacgccgcg ctgtggctgc cgctgatcct cgccaccacg
     1021 ctgctgccga tgcgcgcgat gaagtcgctg ttgatagcgc tgcaattcca tcacaaggct
     1081 gccgaaggcc ggctgattga tcgcgatccg aaatgagcgc ggttccggca ccacggcgcg
     1141 gcgtcgccgg ttttgccatt gtctcgctga tgatggtttc gctgtgcatc ggcctcggcc
     1201 tgtggcaatt gcagcgacgg gccgagaagc acgttctgat cgcgcaactc gccgagcggc
     1261 tcgtggcgtc gccttcgccg ctgcctgcgc cctcgcaatg gagcgcgctg acccgcgcgc
     1321 gcgatgaatt ccgccgcgtc agttttgagg cgatctatgc gccgctcccg gatgcgatgg
     1381 tctacagcgc cggatccggg gtccgcgacg atatttccgg tcccggcacc tgggccttca
     1441 tccccgcgca gctcgcatcc ggcgagaccg tcgcgatcaa caccggcttc gtgcaaaaca
     1501 ccatgcagga ccgcgcccag caggaccgcg cggtggcctg gttcgtcacc ggcgatccgg
     1561 tgcaactgac cggctatatc cgtttccccg agccgcccgg ctcgctgacc ccggccgaaa
     1621 atctcggcaa gaggctgtgg ttcacccggg atcatcaggc tatggcgcgc gcactcggtt
     1681 ggggcgagcg cggcaagccg ctggcgccgt tctatatcga cctcgaacaa ccggtgcctg
     1741 ccagcggcat cccgaagccc ggtgcgctct cggttcacct caaggacgac cacctgcaat
     1801 acgccatcac ctggttcggc ctggcgggtg cggtggtgat tgcgttcggc gtgtggctgc
     1861 gcgggcagcg gcgaagctga gtcttgggga gcgcggctcg ggaacagatc gcccctacgc
     1921 aacctttctc actgttaatc gcggccaacg ccccgttcac tggccattca ccacgatcgt
     1981 cctaagccct cgccaatcag gcagcgcagt gcgcaagtga gtcgacatgt ccaatgtagc
     2041 gggtgatttc gatccgattg cgatcgtggt cgactggatc gacgcatgca gaatgcgcaa
     2101 tctcgatgcg cttctcgacc tgtacgccga tgacgcgaag ctcgaatgcg catgcgaggg
     2161 ctcgaagcat gagggccgcg tcgcgcttgc atcctattgg aggccgcgcc tcgaacaagt
     2221 cgcgccgacg gcattcggcc tggaacaggt aacgcccgcc gccgacggtg tcgtgctcga
     2281 ctacatcagt ttcgaaggca agcacgtccg cgcgttcttt gccttcgatg ccgccgggag
     2341 aattcagcag acgcgctgcg ggcccggtcc ggcgcttcaa acctgacagc cgctggcacc
     2401 tgcgtcgcaa catcagttgc atctgggatc gtttcgtcgg gcgccgcagc atactggcgt
     2461 attggcagcc gaagctcgcc gcgccgccgc cgcgtccctt caagctcgaa cagatttggc
     2521 cggacgccga cggggtcgcc ctggttttca gctatcagca gaagccgccg atacgggtgt
     2581 catttcattt cgatgcggag ggcaagatcg cacgttcgcg atgccgtccc gaacctcagc
     2641 tcccggccgc gaccaccgtt cacagtctgc attcgttttg acgccgcaag gcctcgcccg
     2701 gcattacgtt ccgtccgtct ctccgatcgg caggtggcaa cggatatagg tgcggccctc
     2761 ctcgcgctgg aactcgaagg tgccgctcag cg
//
LOCUS       DATMJB010000027         5085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_825115, whole genome shotgun sequence.
ACCESSION   DATMJB010000027 DATMJB010000000
VERSION     DATMJB010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825115"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VNU48_00600"
     CDS             complement(<1..668)
                     /locus_tag="VNU48_00600"
                     /EC_number="3.1.4.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004114 - 3',5'-cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-nucleotide phosphodiesterase"
                     /protein_id="HWI09808.1"
                     /translation="MILNVLGCSGSIAAGCKTTAFLLDGDVLIDAGTGVGDLELDALA
                     RIDHILISHSHLDHVLAIGLLADAVMRVREPQDRGPIKVHALPETIAALRTHIFNGVI
                     WPDFTRLPSAEQPMLELVPFALGDLLELNGKRIEVLSAVHTVPAVGFAVDGGTDEASG
                     QPRGWWVYTGDTGPNPLLWQRLRSMKVAHLVIETAFSDEERQLARISRHLCPTALGHE
                     LAQL"
     gene            complement(665..2923)
                     /locus_tag="VNU48_00605"
     CDS             complement(665..2923)
                     /locus_tag="VNU48_00605"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWI09809.1"
                     /translation="MLAALLVALGHATGLTPLRFVTEIDLAIADARLRAFMPRTPDAR
                     IVVVDIDEKSLAEIGRWPWSRDHVAALTDELFARQHAAVVGFDVVFAEPDTSSGLPTL
                     ERLAAGSAPIAKTIAQALAPLRAELDFDARFAHALADRNAVLGFYLTGDRDGRRSGLL
                     PAPAFDATLLQGRPIAFTRWDGYAANLPLFAEAAPAAGFFNYVPDPDGLVRAVPLIAE
                     LDGRHYEAIALAMYRLYTGGPAVRPGFATERWLAREYDALQSVLLEQATPQGTQRQAI
                     PVDARVQARVPFRGPGGPHGGSFEYVSASDLLRQRVAPDHLKGKLVLVGSSAPGGYDQ
                     RATPVAEVYPGVEVHASLLSGLLDGRLPAQPDWASAFEVLQLLLVAGLLAWALPRLRA
                     VRGAQLALGLVAVLVAANLWAYRANALLLPLASALLLAAALYVGTTVWGYVVEGRSRR
                     SLARLFGSYVPPELVEEMARDPARYDMHAENRELTMMFCDMRDFTHAAEQLSPEDLRA
                     LVNAFFSTMTAAIRAQRGTLDKYIGDAIMAFWGAPLADPAHAAHATGAALAMAQRLAA
                     LNAQRRARGQPEIGMGIGLNTGTVCVGDMGSSMRRSYTVMGDAVNVAARIEGLTRHYG
                     VAILAGEATRAAAGDAAPGDAVPGWRWVEVDRVRVKGKSGSVTLFTPLAAPPVVTPQM
                     QEEARLWQLALAAYRLQHWGDAQAHLQALLSGFADSALAGLYRRLDERSTHYRATPPP
                     AGWDGAHSFDNK"
     gene            complement(3019..3687)
                     /locus_tag="VNU48_00610"
     CDS             complement(3019..3687)
                     /locus_tag="VNU48_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWI09810.1"
                     /translation="MGKLVVSLDGVVIKEVQITKDKTTLGRRPYNDIVIDNLAVSGEH
                     AVLQMVGADVFIEDLNSTNGTYINGKAVKKQLLSHNDTVEIGKYKIKFIVDDGTDYEK
                     TMIMKPGSRPSEVAGQARSSQFGNSGFGSLGTSTAPASIRVLNGAAAGREVALTKVVT
                     TVGKPGVQVASITKRPGGYVLAHVEGASQPSVNGNPLVGETAHLKNGDVIELAGTQMQ
                     FVQA"
     gene            complement(3749..4531)
                     /locus_tag="VNU48_00615"
     CDS             complement(3749..4531)
                     /locus_tag="VNU48_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HWI09811.1"
                     /translation="MTLEFFSATDTGRARSNNEDSVAVDDATALVVLADGMGGYNAGE
                     VASGMATSFIRSELGRWLTEASYTATDTDVRRAMDICVDNANRAIFNAANSNPQYAGM
                     GTTLVVGVFREQRLLIGHVGDSRGYRLRAQRLTQITHDHSLLQEQIDSGLITPEQAAF
                     SANKNLVTRAVGVEDTVLLETHLHDVLPGDTYLLCSDGLSDMLDDESIAQLLIGAETL
                     PEAAGALIDAANDAGGKDNISVVLARVRGSTGSARSWWPFRR"
     gene            complement(4565..>5085)
                     /locus_tag="VNU48_00620"
     CDS             complement(4565..>5085)
                     /locus_tag="VNU48_00620"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWI09812.1"
                     /translation="IDPDSNTVKVTDFGIARITDAARTRTGMVLGTPSFMSPEQLAGR
                     RVDGRSDLYSLGVMLFQLLTGALPHQSESMAELMRSIVNDTAPDVRTLRPELPEALAN
                     VVALALEKRVELRYADGGQMADDLLAVARLMETQPAPALPPAAVNGASPTARFDATAE
                     FSRGDPRHNADH"
BASE COUNT          713 a         1763 c         1857 g          752 t
ORIGIN      
        1 tcgagttgcg ccagctcgtg cccgagcgcc gtggggcaca gatgccggct gatgcgcgcg
       61 agctgccgct cctcgtcgct gaacgcggtc tcgatcacca ggtgcgcgac cttcatgctg
      121 cgcaggcgct gccacagcag cgggttcggg ccggtgtcgc cggtgtagac ccaccagccg
      181 cgcggctggc cgctcgcttc atcggtgccg ccgtcgacgg caaagcccac cgccggcacg
      241 gtgtgcaccg cgctcagcac ctcgatgcgt ttgccattca gctcgagcag gtcgcccagg
      301 gcgaacggca cgagctcgag catcggctgc tcggcactgg gcaggcgcgt gaagtccggc
      361 cagatcacgc cgttgaagat gtgggtgcgc agcgcggcga tggtctcggg cagcgcgtgg
      421 accttgatcg ggccgcggtc ctgcggttcg cgcacgcgca tcacggcgtc ggcgagcagg
      481 ccgatcgcga ggacgtggtc gaggtgcgag tggctgatca ggatgtggtc gatacgcgcc
      541 agcgcgtcga gctcgaggtc gccgacgccg gtgccggcat cgatcagcac gtcgccgtcg
      601 agcaggaagg ccgtggtctt gcagcccgcg gcgatggacc cggagcaccc gagcacgttg
      661 agaatcattt gttgtcgaag ctgtgggcgc cgtcccagcc ggcgggcggg ggcgtggcgc
      721 ggtagtgagt gctgcgttcg tcgagccggc ggtacaggcc ggcgagagcg gaatcggcga
      781 accccgagag cagcgcctgc aggtgcgcct gcgcgtcgcc ccaatgttgc agccggtacg
      841 ccgcgagcgc aagctgccaa agtcgcgctt cctcctgcat ctgcggggtg accacgggcg
      901 gggcggcgag cggcgtgaaa agcgtcacgg acccgctctt gcccttgacg cgcacgcggt
      961 cgacctcgac ccagcgccag ccgggcaccg cgtcgcccgg cgcggcgtcg ccggcggccg
     1021 cgcgcgtcgc ctcgccggcc aggatcgcga cgccgtagtg gcgcgtcagc ccctcgatgc
     1081 gcgcggccac attgacggcg tcgcccatca ccgtgtagct gcgccgcatg ctcgagccca
     1141 tgtcgccgac gcagacggtg ccggtgttca gcccgatgcc catgccgatc tcgggctggc
     1201 cgcgcgcgcg ccgctgcgcg ttcagcgccg ccaagcgctg cgccatcgcc agcgcggcgc
     1261 cggtggcgtg cgcggcatgg gcggggtcgg cgagcggcgc gccccagaac gccatgatcg
     1321 catcgccgat gtacttgtcg agcgtgccgc gctgcgcgcg aatcgccgcg gtcatcgtcg
     1381 agaagaaggc gttgacgagg gcgcgcaggt cttcgggcga aagctgttcg gcggcgtggg
     1441 tgaagtcgcg catgtcgcag aacatcatgg tgagttcgcg gttctcggcg tgcatgtcgt
     1501 agcgcgccgg gtcgcgcgcc atctcctcga ccagctcggg cggcacatag ctgccgaaca
     1561 ggcgcgccag cgagcgccgg ctgcgcccct cgaccacgta gccccagacc gtcgtgccga
     1621 cgtacaaggc cgcggccagc agcagcgccg aggcgagcgg cagcagcagc gcgttggcgc
     1681 ggtacgccca caggttggcc gccaccagca cggcgacgag gccgagcgcg agctgcgcgc
     1741 cgcgcacggc gcgcagacgt ggcagcgccc aggcgagcag cccggcgacc agcagcagct
     1801 gcagcacctc gaaggcgctg gcccagtcgg gctgcgccgg cagacggccg tcgagcagcc
     1861 ccgagagcag gctcgcgtgc acctcgacgc cggggtagac ctcggccacc ggcgtcgcgc
     1921 gctggtcgta gccgccgggc gcgctgctgc ccaccagcac cagcttgcct ttcaggtggt
     1981 cgggggcgac gcgctgccgc agcagatcgc tcgccgacac gtactcgaac gagccgccgt
     2041 gcggcccgcc gggcccgcgg aacggcacgc gcgcctgcac gcgcgcgtcg accggaatcg
     2101 cctggcgctg cgtgccctgc ggcgtcgcct gctcgagcag cacgctttgc agcgcgtcgt
     2161 actcgcgcgc cagccagcgc tcggtcgcga agccggggcg gaccgccggg ccgccggtat
     2221 agaggcggta catcgccagc gcgatcgcct cgtaatggcg cccgtcgagc tcggcgatca
     2281 gcggcacggc gcgcaccagg ccgtcggggt cgggcacgta gttgaagaag ccggcggccg
     2341 gtgccgcctc ggcgaacagc ggcaggttcg cggcgtagcc gtcccagcgc gtgaacgcga
     2401 tcggccgacc ctgcagcagc gtcgcgtcga acgccggtgc gggcagcagc ccgctgcgcc
     2461 ggccgtcgcg gtcaccggtc aggtagaagc cgagcaccgc gttgcggtcg gccagcgcgt
     2521 gtgcgaagcg ggcatcgaaa tcgagctcgg cgcgcagcgg cgcgagcgct tgtgcgatcg
     2581 ttttcgcgat cggcgccgag ccggcggcca gccgctccag cgtcggcagg ccggagctgg
     2641 tgtcgggctc ggcgaacacc acgtcgaagc cgaccaccgc agcatgctgg cgtgcgaaca
     2701 gctcgtcggt cagcgcggca acgtggtcgc ggctccaggg ccagcggccg atctcggcca
     2761 ggctcttctc gtcgatgtcg acgacgacga tgcgcgcatc cggcgtgcgc ggcatgaagg
     2821 cgcgcagccg cgcgtcggcg atggcgaggt cgatctcggt gacgaagcgc agcggcgtca
     2881 ggccggtggc atgaccgagc gcgacgagca gcgcggccaa catcaacagc gcgcggacgg
     2941 cacgccagaa tcctgggccc gcggccagcg cgagccggcg gtccagccgc gcactgcgtg
     3001 tgcgcggcgg cgtcgggttc aagcttgcac gaactgcatc tgcgtgccgg cgagctcgat
     3061 cacgtcgccg ttcttcaggt gcgccgtttc accgaccagc gggttgccgt tgacgctggg
     3121 ctgcgacgcg ccctcgacgt gcgcgagcac gtagccgccc ggccgcttgg tgatcgacgc
     3181 gacctgcacg ccgggcttgc cgaccgtggt gacgaccttg gtcagcgcga cctcgcgacc
     3241 ggccgccgcg ccgttgagca cgcggatcga cgcgggcgcg gtcgaggtgc cgagcgagcc
     3301 gaaacccgag ttgccgaact gcgagctgcg cgcctggccg gccacctcgg acgggcgcga
     3361 acccggtttc atgatcatcg tcttctcgta gtcggtgccg tcgtcgacga tgaacttgat
     3421 cttgtacttg ccgatctcga ccgtgtcgtt gtggctgagc agctgcttct tgacggcctt
     3481 gccgttgatg taggtgccgt tggtgctgtt caggtcttcg atgaagacat cggcgcccac
     3541 catctgcagc acggcgtgct cgccgctgac cgcgaggttg tcgatcacga tgtcgttgta
     3601 ggggcgccgg ccgagcgtcg tcttgtcctt ggtgatctgc acttccttga tcaccacacc
     3661 gtcgagcgat acgaccagtt tgcccatgct cattgacctc ttgtgtccaa agtctctagc
     3721 ggatcagctg cggcccgcgg cggcggcgtc agcggcggaa cggccaccac gaccgcgcgc
     3781 tgccggtgct gccgcgcacg cgcgccagga ccaccgaaat attatccttg ccgccggcat
     3841 cgttggccgc atcgatcagc gcgccggccg cctcgggcag ggtctcggcg ccgatcagca
     3901 gctgcgcgat gctctcgtcg tcgagcatgt cggacaagcc gtccgagcat agcaggtagg
     3961 tgtcgccggg cagcacgtcg tgcaggtgcg tctccaacag cacggtgtcc tcgacgccga
     4021 ccgcccgcgt cacgaggttc ttgttggccg agaacgcggc ctgctcgggc gtgatcaggc
     4081 cggagtcgat ctgctcctgc agcagcgagt ggtcgtgggt gatctgcgtc aggcgctgcg
     4141 cgcgcaaccg gtagccgcgc gagtcgccga catgcccgat cagcagccgc tgttcgcgga
     4201 acacgccgac caccagcgtc gtgcccatgc cggcgtactg cgggttcgaa ttcgcggcgt
     4261 tgaagatcgc gcggttcgcg ttgtcgacgc agatgtccat cgcgcggcgc acgtcggtgt
     4321 cggtcgcggt gtagctcgcc tcggtcagcc agcgcccgag ctcggagcgg atgaacgagg
     4381 tcgccatgcc gctggcgacc tcgccggcgt tgtagccgcc catgccgtcg gccagcacga
     4441 ccagcgcggt ggcgtcgtcc acggccaccg aatcctcgtt gttgctgcgc gcccgcccgg
     4501 tgtcggtggc gctgaagaac tcgagtgtca tggacggggc gtgtgcgggc atgagggact
     4561 ctgatcagtg gtcggcattg tgcctcggat cgccgcgcga aaactccgcc gtggcgtcga
     4621 agcgggccgt cggcgaggct ccgttcacgg ccgccggcgg cagtgccggc gcgggctgcg
     4681 tctccatcag gcgcgccacc gcgagcaggt cgtccgccat ctggccgccg tcggcgtagc
     4741 gcagctcgac gcgcttctcg agcgccagcg cgacgacgtt ggccagcgcc tccggcagct
     4801 ccgggcgcag cgtgcgcacg tcgggcgcgg tgtcgttgac gatgctgcgc atcagctcgg
     4861 ccatcgactc ggactggtgc ggcagcgcgc cggtcagcag ctggaacagc atcacgccga
     4921 gcgaatacag gtcggagcgg ccatcgacgc ggcgcccggc gagctgctcg ggcgacatga
     4981 acgacggcgt gcccagcacc atgccggtcc gcgtgcgcgc cgcatcggtg atgcgggcga
     5041 tgccgaagtc ggtgaccttc acggtgttgg agtccgggtc gatca
//
LOCUS       DATMJB010000028         2843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_351340, whole genome shotgun sequence.
ACCESSION   DATMJB010000028 DATMJB010000000
VERSION     DATMJB010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351340"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VNU48_00625"
     CDS             complement(<1..275)
                     /locus_tag="VNU48_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWI09813.1"
                     /translation="MAAEEHALTAGDYIVHHLTHLQNSKPKSLVDLSVVNWDSVFFSV
                     LLGVLGCWVMWLVARKVTSGVPGRLQAAVEILVEMVENEAKGIVHNA"
     gene            complement(305..772)
                     /locus_tag="VNU48_00630"
     CDS             complement(305..772)
                     /locus_tag="VNU48_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HWI09814.1"
                     /translation="MSTRGAGLDRFDDEEEDLPVRALTRDEAQALRAKNPPVSPWRVI
                     AVQAAIGGMVVAFAWWLSGSPSVMWSALYGAATAVVPGALMARGMTSPLSSVSPGASA
                     VSVMWWSMVKIGVSVFMLMLAPRLVQPVSWPALLAALVLCMQVYWIALLWRGR"
     gene            complement(966..1868)
                     /locus_tag="VNU48_00635"
     CDS             complement(966..1868)
                     /locus_tag="VNU48_00635"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWI09815.1"
                     /translation="MTAGADVRVAVAAAAQLGESPVWHPGEQALYYCDIAGHQLLRFE
                     PNRGELRHWQFDTDVASFAPRLNGQLLLAMRDGLWHFDPVSGQRARVARAPYDPAVER
                     FNDGKCDPQGRLWVGTIYEPRDPPRASLNCFMHGQIEPRVAGLTVCNGLAWSPNGRTI
                     HWSDTKAHTIYAADFDAATGALSRRRVFASFPVKQPGQALDGYGGRPDGAAMDAEGCY
                     WAAMFEGQRLIRLSPDGEVVREVALPVRCPTMPCFGGPDLRTLYITSSRENRPATELI
                     EQPYAGCVLALEVDVPGLPVNFAL"
     gene            complement(1868..2728)
                     /locus_tag="VNU48_00640"
     CDS             complement(1868..2728)
                     /locus_tag="VNU48_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HWI09816.1"
                     /translation="MTGSAKPSFELHAGALRLALRPDLGGCIAGLWHRDAPVLRSTEP
                     EALSGSRASACYPLLPYSNRLGYRKFRWKGHEHTTQPNFDANPHSLHGVGWQRPWEIV
                     SSSVLEVVLRLRHKGDADWPFAFEASQYFTLTPQSMHVEMVFTNSAEIAQPVGLGWHP
                     YFVKRARSRLHIELAERWDSDATQLPVRKVAQPGIDSDVSHLAFDNCFEGWTGSARIR
                     DEKFSLQLSSSLNRLVVFTPQDRDYFCVEPVSHVSNAIHMADPAAHGLRSLAPGESCE
                     ASMTLEVAVL"
BASE COUNT          483 a         1018 c          922 g          420 t
ORIGIN      
        1 tgcgcgttgt gcacgatgcc cttggcttcg ttctcgacca tctcgacgag gatttcgacc
       61 gccgcctgca aacgaccggg cacgcccgag gtcactttgc gcgccaccag ccacatcacc
      121 cagcagccga gcacgcccag cagcaccgag aagaacaccg aatcccaatt gacgaccgac
      181 agatcgacga gcgacttcgg cttgctgttc tgcaggtggg tcaggtggtg aacgatgtag
      241 tcaccagcgg tgagcgcgtg ctcttcggca gccatgtgat cgagtcctgt acggttctat
      301 tttctcaacg gccgcgccac aacagcgcga tccagtacac ctgcatgcac agcacgagcg
      361 ccgccagcaa ggccggccag ctcacgggct gcacgagtct cggcgccagc atcagcatga
      421 acaccgaaac gccaatcttc accatgctcc accacatgac gctgacggcg ctggcgccgg
      481 gcgacacgct ggacaacggg ctcgtcatgc cgcgcgccat caacgcaccc ggaacgactg
      541 ctgtggcggc gccgtacaac gccgaccaca tcacgctcgg actgcccgac agccaccagg
      601 cgaacgccac caccatcccc ccgatcgcgg cttgcaccgc gatcacccgc cagggagaca
      661 ccggcgggtt cttggcccgc agggcctgcg cttcatcacg cgtcagggcc ctgaccggca
      721 gatcttcttc ttcgtcgtca aagcgatcta agcccgcgcc acgggtgctc atgtcagtca
      781 tggggcgggt cacgaacgct ctcaatacag ccttggattg tacgtgaaaa cccgcccctt
      841 tcgacccgac cccaatcccc gcggttgttg ccgcaccact gtccgacgcg acgcgacgcg
      901 tcagtcaggc caatgcaagt tagtgcgcgc cgagcgcctc gaacgcagca ccaccgcgac
      961 acgcgtcaca gcgcgaagtt gaccggcaac cccggcacgt cgacctcgag cgcgagcacg
     1021 cagccggcat aaggctgctc gatcaactcg gtcgcgggcc ggttttcgcg cgaactcgtg
     1081 atgtacagcg tgcgcagatc cggcccgccg aagcagggca tcgtcgggca gcgaactggc
     1141 agcgcgacct cgcgcacgac ctcgccgtcg ggcgacagcc ggatcagccg ctggccttcg
     1201 aacatcgcgg cccagtagca gccctcggcg tccatcgccg cgccgtcggg gcggccgccg
     1261 taaccgtcca gcgcctgacc gggttgcttg accggaaaac tcgcgaacac gcgtcgccgg
     1321 ctcaacgcgc ccgtggccgc gtcgaagtcg gcggcataga tcgtgtgcgc cttggtgtcg
     1381 ctccagtgga tcgtgcggcc gttcgggctc caggccaggc cgttgcacac cgtcaggccg
     1441 gcgacgcgcg gctcgatctg gccgtgcatg aagcagttca gcgacgcgcg cggcgggtcg
     1501 cgcggctcgt agatcgtgcc gacccagagg cgcccttgcg ggtcgcactt gccgtcgttg
     1561 aagcgttcga ccgccgggtc gtagggcgcg cgggcgaccc gcgcgcgttg accgctcacc
     1621 gggtcgaagt gccacagccc gtcgcgcatc gcgaggagca gctgcccatt cagtcgtggg
     1681 gcgaagctcg cgacatcggt gtcgaactgc cagtggcgca actcaccgcg gttcggctcg
     1741 aagcgcagca actggtggcc ggcgatgtcg cagtagtaca gcgcctgctc gcccgggtgc
     1801 cacaccggcg actcgcccag ctgcgcggcc gccgcgacgg cgacgcggac gtcggcgccc
     1861 gcggtcatca gaggaccgcg acctcgagcg tcatcgacgc ctcgcaactc tcgcccggtg
     1921 cgaggctgcg caggccgtgc gcggccgggt cggccatgtg gatcgcgttc gagacatggc
     1981 tcaccggctc gacgcagaag tagtcgcggt cctgcggcgt gaacacgacc aggcggttca
     2041 gcgacgaact cagctgcagc gagaacttct cgtcgcggat gcgcgccgac ccggtccagc
     2101 cctcgaagca gttgtcgaac gcgaggtgcg acacgtcgct gtcgatgccg ggctgcgcga
     2161 ccttgcgcac cggcaattga gtcgcgtccg aatcccagcg ctcggccagc tcgatgtgca
     2221 ggcggctgcg cgcgcgcttg acgaaatacg ggtgccagcc cagaccgacc ggctgcgcga
     2281 tctcggcgct gttggtgaac accatctcga cgtgcatcga ctgcggcgtc agcgtgaagt
     2341 actggctcgc ctcgaacgcg aacggccagt cggcgtcgcc cttgtgtcgc agcctcagca
     2401 cgacctcgag cacgctgctg gagacgatct cccagggccg ctgccagccc acgccgtgca
     2461 gcgaatgcgg gttcgcgtcg aagttgggct gggtggtgtg ctcgtggccc ttccagcgga
     2521 acttgcggta gcccagccgg ttggaatacg gcaagagcgg gtagcaggcc gacgctcgcg
     2581 agccgctcaa cgcctcgggt tcggtggaac gcaggaccgg cgcgtcgcgg tgccacagcc
     2641 ccgcgatgca gccgccgaga tcggggcgca gcgccagccg caacgcgccg gcatgcaatt
     2701 cgaacgatgg cttagcggat ccagtcatgg ccctcccggc gcgttgagat cgcggcgcag
     2761 gacgcgccca gccgcacgcc ccgtcaccgc ggcccatcct acgccggtcc gccggcgcgc
     2821 ccgacagggt tcagcccaga tcg
//
LOCUS       DATMJB010000029         2620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_409568, whole genome shotgun sequence.
ACCESSION   DATMJB010000029 DATMJB010000000
VERSION     DATMJB010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409568"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="VNU48_00645"
     CDS             <1..1441
                     /locus_tag="VNU48_00645"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWI09817.1"
                     /translation="YESATEVQMQAIPPGLEGADLMVSASTGSGKTASFILPALQRVL
                     LARGDTTKRREKGVIYGPRVLVLCPTRELAMQVAKAAATYGRFVPGLRVATVVGGVPY
                     PAQIAALRGPLDILISTPGRLLDHLNTGKAVLENVEMLVLDEADRMLDMGFIDDINTI
                     AEQIPAARQTVMYSATFLGHVGRLAQNLLRDPKRIDVASHTDTHENIEQRLHWADNVN
                     HKNALLDHILTQKEMEQALVFTSTQRDADWLADRLADMGHHVASLHGGHPQGRRNRVL
                     QGLRNGQLKILIATDVAARGIDVPTISHVINYGLPMKAEDYVHRIGRTGRAGRNGLAV
                     TLGERMDVGMIRRIQQFTTQSIPVQVVAGLEPKSPEPKMFAPRPEGAGARFGNKPFGK
                     PGFGHGAPGKSFGKKPFAPRDRAPFDRNAGRFEPRAAEAPRSPFDRGVATGRVDHNPA
                     GFAPRKPGAPAGKGGGFKPQRPRPNGFTR"
     gene            complement(1503..2384)
                     /locus_tag="VNU48_00650"
     CDS             complement(1503..2384)
                     /locus_tag="VNU48_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWI09818.1"
                     /translation="MPVRYEIVHTTVYHYQQPVTFGEHRVMFRPRDSHDLRVLATDLE
                     VSPEAMVRMIQDPHSNSVALVQPLVPAAELKIVCSFMIEHAHTNNLELPLSPSAEVFP
                     FAYSLEERFDLEGYMRPHYDDPGGHLTAWARQFLRTDGLTGTRELLVQMNQAIRDNFR
                     YVERDEEGTQTPQATLKLNSGSCRDFALLMMEAARRLGIATRFVSGYLYDPALDGGED
                     AGTVGAGATHAWLQAYLPGAGWVPFDPTNNLLGGSQLIRVGVARDPSQAAPIAGSWYG
                     EAGDYLGMTVNVAVKRR"
     gene            complement(2384..>2620)
                     /locus_tag="VNU48_00655"
     CDS             complement(2384..>2620)
                     /locus_tag="VNU48_00655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWI09819.1"
                     /translation="ADNKGAKFDSSKDRNDPFDFGLGAGMVIKGWDEGVQGMQVGGTR
                     LLVIPPELGYGARGAGGVIPPNATLMFEVELLGV"
BASE COUNT          413 a          954 c          885 g          368 t
ORIGIN      
        1 ctacgaatcc gccaccgaag tgcagatgca ggcgatcccg ccggggctgg aaggcgccga
       61 cctgatggtc tcggccagca ccggcagcgg caagaccgcg tcgttcatcc tgccggcgct
      121 gcagcgcgtg ctgctcgccc gcggcgacac caccaagcgg cgcgagaagg gcgtgatcta
      181 cggcccgcgc gtgctcgtgc tgtgcccgac gcgcgaactt gcgatgcagg tcgcgaaggc
      241 cgccgcgacc tacggccgct tcgtgccagg gctgcgtgtc gccacggtcg tcggcggcgt
      301 gccgtacccg gcgcagatcg ccgcgctgcg cggcccgctg gacatcctga tctccacccc
      361 gggccgcctg ctcgaccacc tgaacaccgg caaggccgtg ctcgagaacg tcgagatgct
      421 ggtgctcgac gaagccgacc gcatgctcga catgggcttc atcgacgaca tcaacacgat
      481 cgccgagcag atccccgcgg cgcgccagac ggtgatgtac agcgcgacct tcctcggcca
      541 cgtcggccgg ctcgcgcaga acctgctgcg cgatccgaag cgcatcgacg tcgcctcgca
      601 caccgacacg cacgagaaca tcgagcagcg gctgcactgg gccgacaacg tgaaccacaa
      661 gaacgcgctg ctcgaccaca tcctgaccca gaaggagatg gaacaggcgc tggtcttcac
      721 gagcacgcag cgcgatgccg actggctcgc cgaccgcctg gccgacatgg ggcaccatgt
      781 cgcgtcgctg cacggcggcc acccgcaggg acgccgcaac cgcgtgctgc agggcctgcg
      841 caacggccag ctgaagatcc tgatcgccac cgacgtggcc gcgcgcggca tcgacgtgcc
      901 caccatcagc cacgtgatca actacggcct gccgatgaag gccgaggact acgtgcaccg
      961 catcggccgc accggccggg ccggccgcaa cggcctggcg gtcacgttgg gcgagcgcat
     1021 ggacgtgggc atgatccgcc gcatccagca gttcacgacg cagagcatcc cggtgcaggt
     1081 cgtcgcaggt ctcgagccga agagccccga gccgaagatg ttcgcgccgc gccccgaagg
     1141 cgcgggtgca cgcttcggca acaagccctt cggcaagccc ggtttcggcc atggcgcgcc
     1201 gggcaagagc ttcggcaaga agccgttcgc gccgcgcgac cgcgcgccgt tcgaccgcaa
     1261 cgccggccgc ttcgagccgc gcgccgccga ggcgccgcgc agtccgttcg accgcggcgt
     1321 cgccaccggt cgcgtcgacc acaacccggc cggcttcgcg ccgcgcaagc cgggcgcgcc
     1381 ggccggcaag ggcggcggct tcaagccgca gcgtccgcgc ccgaacggct tcacgcgctg
     1441 aagcgtttca ctcgcgaacg aaaggcccgc actgcgggcc tttttcgtgg ggcgcccgat
     1501 cgtcagcgcc gcttcaccgc cacattgacg gtcatgccga ggtagtcgcc ggcctcgccg
     1561 taccagctgc cggcgatcgg tgcggcctgc gaagggtcgc gcgcgacgcc gacccgaatc
     1621 agctggctgc cgccgagcag gttgttggtc gggtcgaacg gcacccagcc ggcgcccggc
     1681 aggtaggcct gcagccacgc gtgcgtggcg cccgcgccga ccgtgccggc gtcctcgccg
     1741 ccatcgagcg ccgggtcgta caggtaaccc gagacgaagc gcgtcgcgat gccaaggcgc
     1801 cgcgcggcct ccatcatcag cagcgcgaag tcgcggcacg agccgctgtt gagcttgagc
     1861 gtggcctgcg gcgtctgcgt gccttcctcg tcgcgttcga catagcggaa gttgtcgcgg
     1921 atcgcctggt tcatctgcac cagcagctcg cgcgtgccgg tcagcccgtc ggtgcgcagg
     1981 aactggcgcg cccaggcggt caggtgaccg cccggatcgt cgtagtgcgg ccgcatgtag
     2041 ccctcgaggt cgaagcgttc ctccaggctg tacgcgaacg ggaacacctc ggcgctgggc
     2101 gacagcggca gctcgaggtt gttggtgtgg gcgtgctcga tcatgaagct gcagacgatc
     2161 ttcagttccg ccgccggcac cagcggctgc accagtgcga ccgagttcga gtgcgggtct
     2221 tggatcatcc gcaccatcgc ctcggggctg acctcgaggt cggtggccag cacgcgcagg
     2281 tcgtggctgt cgcgcgggcg gaacatcacc cggtgctcgc cgaaggtcac cggctgctgg
     2341 tagtggtaga ccgtcgtgtg gacgatctcg tagcgcacgg gcatcagacg cccagcagct
     2401 cgacctcgaa catcagcgtc gcgttcggcg ggatcacgcc gccggcgccg cgcgcgccgt
     2461 aaccgagctc cggcgggatc accagcagcc gcgtgccgcc gacctgcatg ccttgcacgc
     2521 cttcgtccca gcccttgatg accatgccgg cacccagccc gaagtcgaac ggatcgttcc
     2581 ggtccttgct cgaatcgaac ttcgcgccct tgttgtcggc
//
LOCUS       DATMJB010000030         2425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_113297, whole genome shotgun sequence.
ACCESSION   DATMJB010000030 DATMJB010000000
VERSION     DATMJB010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113297"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..272
                     /locus_tag="VNU48_00660"
     CDS             39..272
                     /locus_tag="VNU48_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HWI09820.1"
                     /translation="MSTRHLSIAGALLAGCALLGGCAIEPWVKPYEREQLADPVMKAS
                     RGTLADKHVEHVRDVREAARGATGVQGGGCGCN"
     gene            259..1359
                     /locus_tag="VNU48_00665"
     CDS             259..1359
                     /locus_tag="VNU48_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HWI09821.1"
                     /translation="MAAIRSRLFRRLAALLGGVLAAGGARAVDLPEDRAEALYHSYTG
                     GGVTANGPALLVRKSVLDKVSLQGSYYVDSVSNASIDVVTTASPYRERRTEYGFSADY
                     AYRDSLITLGTSSSKEPDYTANSVSLDISQESFGGMTTTSLGFTRGTDTVGKHNAPEF
                     ADTAKHWQYRFGVTQILTRQWLASANFEAIADDGYLQSPYRVARVFGAAVPERDPRTR
                     SSRALKFRAVGDLGHHDAVHAEYRYFWDTWGIKAHTAEFGYSRNFGDAILADTFVRYY
                     TQQHAIFYSDNAQQETTYVSRNRQLSSFNDIGLGAKLAYTVRKVPGRYELKLNAGYEY
                     VRFKFSDFTDIRTGSPYATNASVLQLFASGTF"
     gene            1371..1901
                     /locus_tag="VNU48_00670"
     CDS             1371..1901
                     /locus_tag="VNU48_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWI09822.1"
                     /translation="MMMKRCTLLMSLLLAAGAWAADAPAPAVPATLDNRVQDAKSDVI
                     KLNRDLLVLEEELLFPANTQVALFVSMDVGKLFELDSVQIKLDDKVVASYLYTPLEVQ
                     ALHRGGVQRVYLGNLKTGTHELVAFFTGKGPHDRDYKRGATVKFEKDTEAKYIELRIQ
                     DSMGKLQPEFDVKLWK"
     gene            1908..>2425
                     /locus_tag="VNU48_00675"
     CDS             1908..>2425
                     /locus_tag="VNU48_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09823.1"
                     /translation="MRVSSHRRFTLRGLAAAVLAACALSAAAAATDVPNHEIKDPHYG
                     DVLFQFFQDRYFSAVTGLMVSQQFGRVEHHADEAEVLRGGMLLSYGLQREAGEIFAKL
                     IERGAEPKVRDRAWFFLAKICYQRGYLAEADAALAQVGDQLPPPLQEDRALLQANLLM
                     ARADYAGAAGVL"
BASE COUNT          420 a          853 c          796 g          356 t
ORIGIN      
        1 gcgtcgtggg tcgcgcaatg cgcggtggag caactgaaat gagcacgcgt cacctatcga
       61 tcgccggcgc gctgctggcc ggctgcgcgc tgctcggtgg ctgcgcgatc gagccctggg
      121 tcaagcccta cgagcgcgaa cagctggccg atccggtcat gaaggcctcg cgcggcacgc
      181 tcgccgacaa gcacgtcgag cacgtgcgcg acgtgcgcga agccgcgcgc ggcgcgaccg
      241 gcgtgcaggg aggtggttgt ggctgcaatt aggtcgcgcc tgttccgtcg cctggccgcg
      301 ctgctcggtg gcgtgctcgc cgccggcggc gcccgcgcgg tcgacctgcc ggaagaccgc
      361 gccgaggcgc tttaccactc ctacaccggc ggcggcgtca ccgcgaacgg cccggcgctg
      421 ctggtgcgca agagcgtgct cgacaaggtc tcgctgcagg gctcgtacta cgtcgactcg
      481 gtcagcaacg cgtcgatcga cgtggtcacc accgccagcc cgtaccgcga gcgccgcacc
      541 gagtacggct tcagcgccga ctatgcctac cgcgattcgc tgatcacgct gggcacctcg
      601 agcagcaagg agcccgacta caccgcgaac agcgtcagcc tcgacatctc gcaggaaagc
      661 ttcggcggca tgacgacgac cagcctgggc ttcacgcgcg gcaccgacac ggtcggcaag
      721 cacaacgcgc cggaattcgc cgacaccgcg aagcactggc agtaccgctt cggcgtgacg
      781 cagatcctga ccaggcagtg gctggccagc gcgaacttcg aggcgatcgc cgacgacggc
      841 tacctgcaaa gcccgtaccg cgtggcgcgc gtgttcggcg ccgcggtgcc ggagcgcgac
      901 ccgcgcacgc gctcgtcgcg cgcgctgaag tttcgcgcgg tcggcgacct cggtcaccac
      961 gacgcggtgc acgccgagta ccgctacttc tgggacacct ggggcatcaa ggcgcacacg
     1021 gccgagttcg gctacagccg caacttcggc gatgcaatcc tggccgacac cttcgtgcgc
     1081 tactacaccc agcagcacgc catcttctac agcgacaacg cacagcagga aacgacctac
     1141 gtgtcgcgca accgccagct gagcagcttc aacgacatcg gcctgggcgc gaagctcgcc
     1201 tacacggtgc gcaaggtgcc gggccgctac gagctcaagc tcaacgccgg ctacgagtac
     1261 gtgcgcttca agttcagcga cttcaccgac atccgcaccg gcagcccgta cgcgaccaac
     1321 gccagcgtgc tgcagctgtt cgcgtcgggc accttctgat gagccacgcc atgatgatga
     1381 aacgctgcac cctcctgatg tcgctgctgc tcgccgccgg cgcgtgggcc gccgacgcgc
     1441 cggcgccggc agtgccggcc acgctagaca accgcgtgca agacgcgaag agcgacgtga
     1501 tcaagctgaa ccgcgacctg ctcgtgctcg aagaggagct gctgttccca gccaacacgc
     1561 aggtcgcgct gttcgtctcg atggacgtcg gcaagctgtt cgaactcgac tcggtgcaga
     1621 tcaagctcga cgacaaggtc gtggccagct acctgtacac gccgctcgag gtgcaggccc
     1681 tgcaccgcgg cggcgtgcag cgcgtctacc tgggcaacct gaagaccggc acgcacgagc
     1741 tggtcgcgtt cttcaccggc aagggcccgc acgaccgcga ctacaagcgc ggcgcgaccg
     1801 tcaagttcga gaaggacacc gaggcgaagt acatcgagct gcgcatccag gattcgatgg
     1861 gcaagctgca gcccgaattc gacgtgaagc tctggaagta gacgccgatg cgcgtgtcgt
     1921 cacatcgtcg cttcacgctg cgcggcctcg ccgccgcggt gctggccgcc tgcgcgttga
     1981 gcgcagctgc ggctgcaacc gatgtgccga accacgagat caaggatcct cattacggcg
     2041 acgtgctgtt ccagttcttc caggatcgct acttcagcgc cgtcaccggc ctgatggtct
     2101 cgcagcagtt cggccgcgtc gaacaccatg ccgacgaggc cgaggtgctg cgcggcggca
     2161 tgctgctgtc gtacggcctg cagcgcgagg ccggcgagat ctttgcaaag ctgatcgaac
     2221 gcggcgccga acccaaggtg cgcgaccgcg cctggttctt cctcgcgaag atctgctacc
     2281 agcgcggcta tctggccgag gccgacgcgg cgctggcgca ggtcggcgac cagctgccgc
     2341 cgccgctgca ggaagaccgc gcgctgctgc aggccaacct gctgatggcc cgcgccgact
     2401 acgccggcgc ggccggcgtg ctgag
//
LOCUS       DATMJB010000031         7805 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_824175, whole genome shotgun sequence.
ACCESSION   DATMJB010000031 DATMJB010000000
VERSION     DATMJB010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7805
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824175"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1000)
                     /locus_tag="VNU48_00680"
     CDS             complement(152..1000)
                     /locus_tag="VNU48_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HWI09824.1"
                     /translation="MSELSAARAGEQDGPAASPAVIDGIAVARLYGEPLFAMPTDLYI
                     PPDALEVFLEAFEGPLDLLLYLIRRQNFNILDIPMASVTRQYLAYVEQIRKTNLELAS
                     EYLLMAAMLIEIKSRMLLPPKKSADGAEPEDPRAELVRRLIEYEQMKLAAARIDQLPL
                     LGRDFLRAQVVIEQSLVPRFPDVAADELRAAWADIVQRARLNQHHHITREQLSVREHM
                     SIVLRALQGRRFALFEDLFDVSRGPQVLVVTFIAMLELARERLLEVTQAEAFAPIYVR
                     LAYAPV"
     gene            complement(1043..1243)
                     /locus_tag="VNU48_00685"
     CDS             complement(1043..1243)
                     /locus_tag="VNU48_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HWI09825.1"
                     /translation="MPLFWKAYKSDVTSFIEAMKANKPTLEDEQRQGRQLLWDKRIDR
                     DAQAEYGAARVPQQPYVYQTKT"
     gene            1379..2896
                     /locus_tag="VNU48_00690"
     CDS             1379..2896
                     /locus_tag="VNU48_00690"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HWI09826.1"
                     /translation="MQEIEIKLQVPAARRAEVEAAVAGRTPGARVRLQAWYHDCADRR
                     LAAGGLALRLRREGRRWVQTLKGAGDDGLTRAEHNVPRGAAAAMPTLDPALHAGTPVG
                     ERLLALLTAAPSATLEPLYRTDIRRRARVRPVRWPGGPSARVELAFDRGRIEAGARVL
                     EVSELEIELLAGAPRAVIETARRWGLRHGLWLDLRSKAERGDLLARGETVAPARSAQP
                     LKLDVAMSAAQAWQAVLRNCADQILANASQIASGAHDAEHVHQLRVGLRRLRSALALF
                     EPADASAALGGGASALFRQLGAARDRVVIEGEFAAALRAAMREAGVANEAPVPAASDT
                     EAAPPELLRLHASQSLLLDLIEAMHPGAGAEAGRADALSPLRDRMAARLNHWHRQVVA
                     DAKHYVELDDAQRHRLRKRAKRLRYATEFCGALFERRAVRRYLKALRDLQERLGAVSD
                     TTMAMCAFAPRADTEPAAMFALGWLAARRAALVAASGPELKRFAKVRRYWKETRG"
     gene            complement(2898..3770)
                     /locus_tag="VNU48_00695"
     CDS             complement(2898..3770)
                     /locus_tag="VNU48_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HWI09827.1"
                     /translation="MNATSVHEFADASWADGPTGGVEAVLEDGAVLTFPHLSFALSDA
                     ERRFLDPRWADGKAKNISLRWPGGEMRGAAGDKADLLALRAMILRYTECSEALALRLF
                     PHYRGHLRRGNTSFRPVDVAGRATSWRQDDTRLHVDAFPSNPMQGTRLLRVFCNVNPA
                     GRPRAWRVGEPFEAHAKRYLPAIRRPLPGSAWLLATTGVTKRRRTEYDHVMLQLHDRA
                     KADAEFQRSSPQIRVDFAPGTTWVVFSDQVLHAAMGGQHMMEQTFYLDVARQQRPEAS
                     PRGVLERLLGRALR"
     gene            complement(3767..5821)
                     /gene="metG"
                     /locus_tag="VNU48_00700"
     CDS             complement(3767..5821)
                     /gene="metG"
                     /locus_tag="VNU48_00700"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWI09828.1"
                     /translation="MTRKLFVTTALPYANGPFHIGHMMEYIQADIWVRFQRMRGVEVH
                     FVCADDAHGAPIMIAAEKAGLTPQDFVAQVAAGRAKYLDGFHIGFDNWHSTDAPENHE
                     LAQDIYRALRKQGLIAERTIEQFYDPVKGMFLPDRYIKGECPRCGTKDQYGDNCDNCG
                     AVYAPTDLKNPYSTLSGATPVMKRSDHYFFQLSAQRCVDFLKDWTATPGRLQPEVLNK
                     IREWFASDDEVGKGGHVGLNDWDISRDAPYFGIEIPDAPGKYFYVWLDAPVGYLASLK
                     NYLGKIGIDYDAYMADPDVEQIHFIGKDIVTFHTLFWPAMLHFSGRKVPSHIFVHGHL
                     HLSGEKMSKSRGTGISPLKYLELGMNPEWLRYYIAAKLNGRVEDLDFNPDDFLARVNA
                     DLVGKYINIASRAAGFLGKRFGGLLSADLGVEGRALLDGLRAQCATIEELYEEREFAK
                     ALREVMLLADRVNEYVDRHKPWELAKQAGQDALLHDVCSVCIEAFRLLTIYLKPVLPA
                     LAAQVEAFLRIEPLRFADATRALGGHTIGAYTHLLQRVDAKQLDALLEPEVAEVPPPG
                     GEALAAEIKVDDFAKIDLRIARIEHCEAVEGSTKLLRLTLDVGEGRMRNVFSGIASAY
                     RPADLVGKFTVMVANLAPRKMKFGISEGMVLAASHADEKVEPGLYILEPWPGAKPGMR
                     VR"
     gene            complement(5898..7484)
                     /locus_tag="VNU48_00705"
     CDS             complement(5898..7484)
                     /locus_tag="VNU48_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein D"
                     /protein_id="HWI09829.1"
                     /translation="MQRRTLLRHAGGAAAFSVTGLLWPTAHAAGLKPLGKPQAFGLAW
                     LKGQARALAAAPYKPAQNKLPAAITGLDWDQYQAIRFKPEHALWADAKLRFQIELFHL
                     GLYFKRPVQVFEVANGQAQQLAYDPAMFDYGKSGLNGARQPADLGFAGFRFKFHLAPQ
                     ADIAAFLGASYFRATSGTRQYGLSARGLAVDTGMARAEEFPEFTHFWFERPAPDSNTL
                     VVHALLDSPSIAGAYRFAITPGDTTLMEIDAALYPRKEIERLGIAPCTSMFQTGENDR
                     RKGNDWRPEIHDSDGLAMHTGSGEWIWRPLRNPRALSFNAFADRSPRGFGLLQRDHDF
                     ANYQDDGVFYERRPSLWVEPKGDWGVGAVDLVEIPTIDETFDNIVAFWNPATKPQPGQ
                     ELLIGYRLYWGREAPAQSPLARVVATRTGLGGVIGKKRGYFSWRFAVDFAGGDFALLD
                     PKTMVEPVISASRGRVEITSARPLGAVNGWRAMFDLVPDANSVEPIALRLFLRADGQP
                     LTETWLYEYAPPPPAERALE"
     gene            7601..>7805
                     /locus_tag="VNU48_00710"
     CDS             7601..>7805
                     /locus_tag="VNU48_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HWI09830.1"
                     /translation="MPAPSEAALRAALETVIDPNTGSDFVSTRQLKNLRVEGGDVAFD
                     VELGYPAKSQIGALRKALIAAARS"
BASE COUNT         1110 a         2706 c         2811 g         1178 t
ORIGIN      
        1 ttccgcgacc ccaacggcta cgtcatcgag ctgtgcgcca agcgccccga gcacgacgcg
       61 ctgatggacc cggcccgcaa cggcgcccgc gccaagctgc agcgctggac ggccgccaag
      121 gccggcgcgt cggtctgacc actgcccggc gtcacacggg cgcgtaggcc agccgcacgt
      181 agatcggcgc gaacgcctcg gcctgcgtga cctcgagcag ccgctcgcgc gcgagctcga
      241 gcatcgcgat gaaggtcacg accagcacct gcgggccgcg gctcacgtcg aacaggtcct
      301 cgaacagcgc gaagcgccgg ccctgcagcg cgcgcagcac gatgctcatg tgctcgcgca
      361 cgctgagctg ctcgcgcgtg atgtggtggt gctggttcag tcgggcgcgc tgcacgatgt
      421 cggcccaggc ggcgcgcagc tcgtcggcgg ccacgtcggg gaaacgcggc accagcgact
      481 gctcgatcac gacctgcgcg cgcaggaagt cgcgccccag cagcggcagc tgatcgatgc
      541 gcgcggccgc cagcttcatc tgctcgtact cgatcagtcg gcgcaccagc tcggcgcgcg
      601 ggtcttcggg ctcggccccg tccgcgctct tcttcggcgg cagcagcatg cgcgacttga
      661 tctcgatcag catcgccgcc atcaagaggt attcgctggc gagctcgaga ttggtcttgc
      721 ggatctgctc gacgtaggcc aggtactggc gcgtcacgct cgccatcggg atgtcgagga
      781 tgttgaagtt ctggcgccgg atcaggtaca gcagcaggtc cagcgggccc tcgaaggcct
      841 ctagaaacac ctccagcgca tccggcggga tgtacaggtc ggtgggcatc gcgaacagcg
      901 gctcgccgta caggcgcgcc accgcgatgc cgtcgatcac cgcggggctg gcggccgggc
      961 cgtcctgctc gcccgcgcgc gcagccgaca gttcagacac ctgcggcggc gccgcatcgt
     1021 cgctcacgac ggatctcgaa gctcaggtct tggtctggta gacgtagggc tgctgcggca
     1081 cgcgggcagc gccgtactcg gcctgggcgt cgcggtcgat gcgcttgtcc cacagcaact
     1141 ggcggccctg gcgctgctcg tcctccagcg tcggcttgtt ggccttcatc gcctcgatga
     1201 acgaggtgac gtcggatttg taggctttcc agaagagtgg catggcagct tggggcgccc
     1261 ccgtcttggg tgctggcgct ggagtggaag tcgcgatttt agcgggggag cgcgggagcg
     1321 tcgtccttcg cacccgggag tcatcaagat cgacgccgcg gcatgtcacg atcccgccat
     1381 gcaagagatc gagatcaagc tccaggtgcc ggcggcgcgc cgtgccgagg tcgaggccgc
     1441 ggtggccggg cgcacgcccg gcgcgcgggt gcgcctgcag gcctggtacc acgactgcgc
     1501 cgaccgccgc ctggccgccg gcggtctggc gctgcgcctg cgacgcgagg gccgccgctg
     1561 ggtgcagacg ctcaagggcg cgggagacga cggcctgacg cgcgccgagc acaacgtgcc
     1621 gcgcggcgcc gcggccgcga tgcccacgct cgatccggcg ctgcatgccg gcacgcccgt
     1681 gggcgagcgg ctgctcgcgc tgctcaccgc agcgccgagc gcaacgctcg agccgctgta
     1741 ccgcaccgac atccgccggc gcgcccgcgt ccgcccggtg cgctggccgg gtgggccaag
     1801 cgcgcgcgtc gagctggcgt tcgaccgcgg ccgcatcgag gccggcgcgc gcgtgctcga
     1861 ggtgagcgag ctcgagatcg aactgctggc cggcgcgccg cgcgcggtga tcgagaccgc
     1921 gcggcgctgg gggctgcgcc atggcctgtg gctggacctg cgcagcaagg ccgagcgcgg
     1981 cgacctgctg gcgcgcggcg agaccgtcgc gccggcgcgc agcgcgcagc cgctgaagct
     2041 cgacgtggcg atgagcgcgg cgcaggcgtg gcaggccgtg ctgcgcaact gtgccgacca
     2101 gatccttgcg aacgcgagcc agatcgccag cggcgcccac gacgccgaac atgtgcatca
     2161 gctgcgcgtc ggcctgcggc gcttgcgcag cgcgctcgcg ctgttcgaac cggccgacgc
     2221 gagcgccgcg ctcggcggcg gggcgagcgc gctgttccgg caactcggtg cagcgcgcga
     2281 ccgggtcgtg atcgaaggcg agttcgccgc tgcgctgcgc gcggcgatgc gcgaggccgg
     2341 cgtggcgaac gaggcaccgg tgcccgccgc gtcggacacc gaggccgcgc cgcccgaact
     2401 gctgcgcctg cacgccagcc agagcctgct gctcgacctg atcgaggcga tgcaccccgg
     2461 cgccggcgcc gaggcaggcc gcgccgacgc gctgtcgccg ctgcgcgacc gaatggccgc
     2521 gcgcctgaac cactggcacc gccaggtcgt cgccgatgcg aagcactacg tcgaactcga
     2581 cgacgcgcaa cgccatcgcc tgcgcaagcg cgcgaagcgg ctgcgctacg cgaccgagtt
     2641 ctgcggcgcg ctgttcgagc ggcgcgcggt gcgccgctac ctgaaagcgt tgcgcgactt
     2701 gcaggagcgc ctcggcgcgg tcagcgacac gacgatggcg atgtgcgcgt tcgcgccgcg
     2761 cgccgacacc gagccggcgg cgatgttcgc gctcggctgg ctcgcggcgc ggcgcgcggc
     2821 gctggtcgcc gcaagcggcc ccgagctgaa gcgcttcgcg aaggtccggc gttactggaa
     2881 ggagacgcgg ggctgaatca gcgcagcgcg cgcccgagca gccgctccag cacgccgcgc
     2941 ggcgacgcct cggggcgctg ctggcgcgcc acgtcgaggt agaaggtctg ctccatcatg
     3001 tgctggccgc ccatcgcggc gtgcagcacc tggtcgctga acacgaccca ggtcgtgccg
     3061 ggcgcgaagt cgacacggat ctgcgggctg ctgcgctgga actcggcgtc ggccttggcg
     3121 cggtcgtgca gctgcagcat gacgtggtcg tactcggtgc ggcggcgctt cgtgacgccg
     3181 gtggtcgcca gcagccaggc cgagcccggc agcggccgcc ggatcgccgg caggtagcgt
     3241 ttcgcatgcg cctcgaacgg ctcgccgacg cgccacgcgc gcggccggcc cgcagggttg
     3301 acgttgcaga acacgcgcag cagccgcgtg ccctgcatcg ggttcgacgg gaacgcgtcg
     3361 acgtgcagcc gcgtgtcgtc ctggcgccag ctggtcgcgc ggccggccac gtcgaccggc
     3421 cggaacgagg tgttgccgcg ccgcaggtga ccgcggtagt gcggaaacag acgcagcgcg
     3481 agcgcctcgc tgcactcggt gtagcgcagg atcatcgcgc gcagcgcgag caggtcggcc
     3541 ttgtcgccgg ccgcgccgcg catctcgccg ccgggccagc gcagcgagat gttcttcgcc
     3601 ttgccgtcgg cccagcgcgg gtcgagaaag cggcgttcgg catccgacag cgcgaacgac
     3661 aagtggggga acgtcaggac cgcgccgtcc tcgagcaccg cctcgacgcc gccagtcggg
     3721 ccgtcggccc agctcgcgtc ggcgaactcg tgcaccgacg tggcgttcat cgcacgcgca
     3781 tccccggctt cgcgccgggc cagggttcga ggatgtacag gcccggctcg accttctcgt
     3841 cggcgtgcga cgccgcgagc accatgcctt cgctgatgcc gaacttcatc ttgcgcggcg
     3901 ccaggttcgc gaccatcacg gtgaacttgc cgaccaggtc ggcgggccgg taggccgatg
     3961 cgatgccgct gaagacgttg cgcatcctgc cttcgccgac gtcgagcgtc aggcgcagca
     4021 gcttggtcga gccctcgacc gcctcgcagt gctcgatgcg tgcgatgcgc aggtcgatct
     4081 tcgcgaagtc gtcgaccttg atttcggcgg cgagcgcctc gccgcccggc ggcggcacct
     4141 cggcgacctc gggctcgagc agcgcgtcga gctgcttcgc gtcgacgcgc tgcagcaggt
     4201 gcgtgtaggc gccgatcgtg tggccaccga gcgcgcgcgt cgcatcggcg aagcgcagcg
     4261 gctcgatccg caggaacgcc tcgacctgtg cggcgagcgc cggcagcacc ggcttcaggt
     4321 agatcgtcag cagccggaat gcctcgatgc agaccgagca gacgtcgtgc agcaacgcgt
     4381 cctgcccggc ctgcttggcc agttcccagg gcttgtgccg gtcgacgtac tcgttgacgc
     4441 ggtcggccag cagcatcacc tcgcgcagcg ccttcgcgaa ctcgcgctcc tcgtacagtt
     4501 cctcgatcgt cgcgcactgc gcgcgcagac cgtcgagcag cgcgcggccc tcgacgccga
     4561 ggtcggccga cagcagcccg ccgaagcgct tgccgaggaa gcccgccgcg cggctggcga
     4621 tgttgatgta cttgccgacc aggtcggcgt tgacgcgtgc caggaagtcg tcggggttga
     4681 agtcgaggtc ctcgacgcgg ccgttcagct tcgcggcgat gtagtagcgc agccactcgg
     4741 ggttcatgcc gagttccagg tacttcagcg gcgagatgcc ggtgccgcgg ctcttgctca
     4801 tcttctcgcc gctgaggtgc aggtggccgt gcacgaagat gtgactcggt accttgcggc
     4861 cgctgaagtg cagcatcgcc ggccagaaca gcgtgtggaa ggtgacgatg tccttgccga
     4921 tgaagtggat ctgctcgaca tccgggtcgg ccatgtaggc gtcgtagtcg atgccgatct
     4981 tgccgaggta gttcttcagc gacgcgaggt agcccaccgg cgcgtccagc cagacataga
     5041 aatacttgcc cggcgcatcc gggatctcga tgccgaagta cggcgcgtcg cggctgatgt
     5101 cccaatcgtt caggccgacg tggccgccct tgcccacttc gtcgtcgctg gcgaaccact
     5161 cccggatctt gttcagcacc tcgggctgca gccggcccgg cgtcgcggtc cagtccttca
     5221 ggaagtcgac gcagcgctgc gccgagagct ggaagaaata gtgatcgctg cgcttcatca
     5281 ccggcgtcgc gccgctcagc gtcgaatacg ggttcttcag atcggtgggc gcgtagacgg
     5341 cgccgcagtt gtcgcagttg tcgccgtact ggtctttggt gccgcacctc gggcactcgc
     5401 ccttgatgta gcggtcgggc aggaacatgc ccttgaccgg gtcgtagaac tgctcgatcg
     5461 tgcgttcggc gatcaggccc tgcttgcgca gcgcgcggta gatgtcctgc gcgagttcgt
     5521 ggttctcggg cgcgtcggtc gagtgccagt tgtcgaagcc gatatggaag ccgtcgagat
     5581 atttcgcgcg gcccgccgcg acctgcgcaa cgaagtcctg cggcgtcagc cccgccttct
     5641 cggccgcgat catgatcggc gcgccgtgcg catcgtcggc gcagacgaaa tgcacctcaa
     5701 cgccccgcat gcgctggaac cgcacccaga tgtcggcctg gatgtactcc atcatgtggc
     5761 cgatgtggaa cggcccgttg gcgtagggca gggcggtggt gacgaacagc ttgcgggtca
     5821 tgcgaaacgc cggcagcgcc ggcgacgaag tgaaccggca attttacggg cgcccggccc
     5881 gcgcgcccgg cgcaggttca ttcgagcgcg cgttcggccg gcggtggcgg cgcgtactcg
     5941 tagagccagg tctcggtcag cggctggccg tcggcgcgca ggaacaggcg cagcgcgatc
     6001 ggctcgacgc tgttcgcatc gggcaccagg tcgaacatcg cgcgccagcc gttcaccgcg
     6061 cccagcggcc gcgccgaggt gatctcgacc cggccgcgcg acgcgctgat gaccggctcg
     6121 accatcgtct tcgggtcgag cagcgcgaag tcgccgcccg cgaagtcgac cgcgaagcgc
     6181 cacgagaagt agccgcgctt cttgccgatc acgccgccca gcccggtgcg cgtggcgacc
     6241 acgcgcgcca gcggcgactg cgccggcgcc tcgcggcccc agtacaggcg gtagccgatc
     6301 agcagttcct ggccgggctg gggcttggtt gcagggttcc agaacgcgac gatgttgtcg
     6361 aaggtctcgt cgatcgtcgg gatctcgacc aggtcgaccg cgccgacgcc ccagtcgccc
     6421 ttgggctcga cccacaggct cgggcggcgc tcgtagaaca cgccgtcgtc ctggtagttc
     6481 gcaaaatcgt ggtcgcgctg cagcaggccg aagccgcgcg ggctgcggtc ggcgaaggcg
     6541 ttgaagctga gtgcgcgcgg gttgcgcagc ggccgccaga tccattcgcc gctgccggtg
     6601 tgcatggcca ggccgtcgga atcgtggatc tccgggcgcc agtcgttgcc tttgcggcgg
     6661 tcgttctcgc cggtctggaa catgctcgtg cacggcgcga tgccgagccg ctcgatctcc
     6721 ttgcgcgggt acagcgcggc gtcgatctcc atcagcgtcg tgtcgccggg cgtgatcgcg
     6781 aaacggtagg cgccggcgat gctgggcgag tcgagcagtg cgtggacgac cagcgtgttc
     6841 gagtcgggcg cgggccgctc gaaccagaag tgcgtgaact cggggaactc ctcggcgcgc
     6901 gccatgccgg tgtcgacggc caggccgcgc gccgacaggc cgtactggcg cgtgccgctg
     6961 gtcgcgcgga agtagctcgc gccgaggaag gccgcgatgt cggcctgcgg cgcgaggtgg
     7021 aacttgaatc gaaagccggc gaagcccagg tccgccggct ggcgcgcgcc gttcaggccg
     7081 ctcttgccgt agtcgaacat cgccgggtcg taggcgagct gctgggcctg accgttcgcg
     7141 acctcgaaca cctgcaccgg ccgcttgaag tacaggccga ggtggaacag ctcgatctgg
     7201 aagcgcagct tcgcatcggc ccacagtgcg tgctcgggct tgaagcggat cgcctggtac
     7261 tggtcccagt cgagccccgt gatggccgcg ggcagcttgt tctgcgccgg cttgtagggc
     7321 gccgcggcga gtgcgcgcgc ctgccccttg agccacgcga ggccgaaggc ctgcggcttg
     7381 ccgagcggct tcaggccggc cgcgtgggcc gtgggccaga gcaggccggt gacgctgaag
     7441 gcggcggcgc cgccagcgtg gcgaaggagg gtgcgtcgtt gcatcgtgcc ggaatatccg
     7501 aagtggcaat tgcctccatt cttgcctgtc tttgggccgg cgccccgcga cgcctgccgg
     7561 cgtaccgcta gacttcacct ccactcacga cattcgcacc atgcccgctc cctctgaggc
     7621 cgcactgcgc gccgcgctcg agaccgtcat cgaccccaac accggcagcg acttcgtttc
     7681 cacacggcag ctgaagaatc tgcgcgtcga gggcggcgac gtggccttcg acgtcgagct
     7741 cggctacccc gcgaagagcc agatcggcgc cttgcgcaag gcactgatcg cggcggcgcg
     7801 cagcg
//
LOCUS       DATMJB010000032         2796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_824153, whole genome shotgun sequence.
ACCESSION   DATMJB010000032 DATMJB010000000
VERSION     DATMJB010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824153"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VNU48_00715"
     CDS             <1..1063
                     /locus_tag="VNU48_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWI09831.1"
                     /translation="VQGGADNERPPSDGSDTPDDGLVSLGSLFYEPVWLFYRTDSAQR
                     LLHTPALTSLSQLPGWRLNVGARGSGTPNLMRKLIEANHSIDATTLTLVRQSQTPAAM
                     ALVAGDIDALVFASAPESLMVQMLLSTPGIALYDFAQADAYSRLFPFLTPVTLPRGIV
                     DLAGDVPPRDVRLLAPTATLLARAGTHPALLQLFIQAAQQVHGGAGWFQHKGDFPKAA
                     GTEQPLAKEAERFYANGTPVLQRYLPFWLANLIDRMWPVLVTIVAIMLPLSRMLPPLY
                     QFRIRSRIFRWYAQLRRVEAGAGKRPVAELLAELDAIEAHVEEVTVPLSYADELYSLR
                     SHIRMVGDRLRAAAPAGIA"
     gene            1060..2505
                     /locus_tag="VNU48_00720"
     CDS             1060..2505
                     /locus_tag="VNU48_00720"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWI09832.1"
                     /translation="MSGRPRVVIIGCGFAGIEAVRNLSKADVEITLVDRTNHHLFQPL
                     LYQVATAGLSAPAISAPIRHTLRREMKRGNLTVLQAEVSGIDAVGRRVLLDGDDKDAL
                     AYDHLIVAAGATHSYFGHDDWAAYAPGLKTLDDAFKIRARVIGAFERAERCTEPAERE
                     PWITFVVIGAGPTGVEMAGTMTEIAQHTLPQEFRRIDSTHTRVVLLEGSERVLGAFVP
                     RLSQRAREQLERLGVDVRTGCKVTGIDAAGVTYERQEGGAMQRFRLPSRTVIWAAGVA
                     GSPLGQALATGAGAILDRAGRVVVRPDLSLTDHPEIAVIGDLASAMSHPGASAPRDAK
                     PVPVPGVSPAAKQMGRAAAANLLRRLRGQATVPFRYIDYGNLATVGRKAAVVDLTVPV
                     FGALRFSGFLAWLFWLFAHIWFLIGFRNRLIVMLDWGWAYWTYERSARVVAEPPTAPS
                     LPSRVVAEPPTAPSLPSRVVAEPPTAPPSHR"
     gene            2618..>2796
                     /locus_tag="VNU48_00725"
     CDS             2618..>2796
                     /locus_tag="VNU48_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HWI09833.1"
                     /translation="MDTRTSPYRLRIEKVRDALKRHGLAAVLVPSSDPHLSEYLPERW
                     QGRQWLSGFTGSMGTL"
BASE COUNT          388 a         1035 c          971 g          402 t
ORIGIN      
        1 cgtgcagggc ggcgccgaca acgaacggcc gccgagcgac ggcagcgaca ctcccgacga
       61 cggcctggtc tcgctcggca gcctgttcta cgagccggtc tggctgttct accgcaccga
      121 ctcggcgcag cggctgctgc acacgccggc gctgaccagc ctgtcgcagc tgccgggctg
      181 gcgcctgaac gtcggcgcca ggggcagcgg cacgcccaac ctgatgcgca agttgatcga
      241 ggcgaaccac agcatcgatg cgacgacgct gacgctggtg cgccagtcgc agacaccggc
      301 cgcgatggcg ctggtggccg gcgacatcga cgcgctggtg ttcgcttcgg cgcccgagtc
      361 gctgatggtg cagatgctgc tgagcacgcc cggcatcgcg ctttacgact tcgcgcaggc
      421 cgacgcgtat tcgcgcctgt tcccgttcct gacgccggtg acgctgccgc gcggcatcgt
      481 cgacctcgcc ggcgacgtgc cgccgcgcga tgtgcgcctg ctggccccca cggcgacgct
      541 gctggcgcgc gccggcacgc atccggcgct gctgcagctg ttcatccagg ccgcccagca
      601 ggtgcacggc ggtgccggct ggttccagca caagggcgac ttcccgaagg cggccggcac
      661 cgagcagccg ctcgcgaagg aggccgagcg cttctacgcc aacggcacgc cggtgctgca
      721 gcgctacctg ccgttctggc tcgcgaacct gatcgaccgg atgtggccgg tgctggtgac
      781 gatcgtcgcg atcatgctgc cgctgtcgcg catgttgccg ccgctgtacc agttccgcat
      841 ccgctcgcgc atcttccgct ggtatgcgca gttgcgccgt gtcgaggccg gcgccggcaa
      901 gcggccggtc gccgaactgc tggccgagct cgacgcgatc gaggcgcatg tcgaagaggt
      961 gaccgtgccg ctgagctatg ccgacgagct gtactcgctg cgcagccaca tccggatggt
     1021 gggcgaccgg ctgcgcgcag cggcaccggc gggaatcgca tgagcgggcg gcctcgtgtc
     1081 gtcatcatcg gctgcggctt cgccggcatc gaggcggtgc gcaacctgtc gaaggcggat
     1141 gtcgagatca ccttggtcga ccgcaccaac caccacctgt tccagccgct gctgtaccag
     1201 gtcgcgaccg ccggcctgag cgcgccggcg atcagtgcgc cgattcgcca cacgctgcgg
     1261 cgcgagatga agcgcggcaa cctgaccgtc ctgcaggccg aggtgagcgg catcgacgcc
     1321 gtcgggcgcc gcgtgctgct cgacggcgac gacaaagacg ccctcgccta cgaccacctg
     1381 atcgtcgccg ccggcgcgac ccacagctac ttcgggcacg acgactgggc cgcctacgca
     1441 ccggggctga agacgctcga cgacgcgttc aagatccgcg cccgcgtgat cggcgcgttc
     1501 gaacgtgccg agcgctgcac cgagccggcc gagcgcgaac cgtggataac tttcgtcgtg
     1561 atcggcgccg gcccgaccgg cgtcgagatg gccggcacga tgaccgagat cgcgcagcac
     1621 acgctgccgc aggagttccg ccgcatcgac tcgacgcaca cgcgggtcgt tctgctcgaa
     1681 ggctccgagc gcgtgctcgg cgcgttcgtg ccgcgcctgt cgcagcgcgc gcgcgagcag
     1741 ctcgagcggc tcggcgtcga cgtgcgcacc ggctgcaagg tgaccggcat cgacgccgcc
     1801 ggcgtgacct acgagcgcca ggagggcggc gcaatgcagc gcttccggct gccgagcagg
     1861 accgtgatct gggccgccgg cgtcgccggc tcaccgctcg gccaggcgct cgcgacgggc
     1921 gccggcgcga tcctcgaccg cgccggccgc gtcgtcgtgc ggcccgacct gagcctgacc
     1981 gaccaccccg agatcgcggt gatcggcgac ctcgcctcgg cgatgagcca cccgggcgcc
     2041 tcggcgccgc gcgacgccaa gcccgtgccg gtgcccggcg tcagcccggc cgcgaagcag
     2101 atgggccgcg ccgccgccgc gaacctgctg cgccggctgc gcgggcaggc caccgtgccg
     2161 ttccgctaca tcgactacgg caacctcgcc acggtcggcc gcaaggccgc ggtcgtcgac
     2221 ctgaccgtgc cggtgttcgg cgcgctgcgc ttctcgggct tcctcgcgtg gctgttctgg
     2281 ctgttcgcgc acatctggtt cctgatcggt tttcgcaacc gcctgatcgt gatgctcgac
     2341 tggggctggg cctactggac ctacgagcgc tcggcgcggg tggtggccga gccaccaacc
     2401 gcgccctcgc tgccttcgcg ggtggtggcc gagccaccga ccgcgccctc gctgccttcg
     2461 cgggtggtgg cggagccgcc gaccgcgccg ccctctcatc gatgacccac aggccgcgcc
     2521 ggaccagctg aaggaactgg aagccgagtt gccgccgggc ccgaactcct agaatcgggc
     2581 cgccggcaca accgacacct ctttccggag ccacacgatg gacacgcgca cctcccccta
     2641 tcgcctgcgc atcgagaagg tccgcgacgc gctgaaacgc cacggcctgg ccgcggtgct
     2701 ggtgccgtcg agcgacccgc acctgagcga gtacctgccc gagcgctggc aggggcggca
     2761 atggctgtct ggcttcaccg gctcgatggg cacgct
//
LOCUS       DATMJB010000033         2612 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_823493, whole genome shotgun sequence.
ACCESSION   DATMJB010000033 DATMJB010000000
VERSION     DATMJB010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823493"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VNU48_00730"
     CDS             complement(<1..838)
                     /locus_tag="VNU48_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWI09834.1"
                     /translation="MKAKTWKAVTAEAKLLLIGIPLFLWTIIPVYHLFLFAISSKDSA
                     TSGRLWPKEPTLQNFSIVFNRQHFYLDHFWVQLGNSVLIAVAVAAITLFVSTAAAFAI
                     SRLKVRGGRTVMNLALFTYFIPAAFLAVPMYKTMGNYGLLNSQWSLILAMVTIASPYC
                     IWVLKQASDKLPWELDEAARMDGASPLQLFRLVYLPLMVPSLVAIFTYALLLAWNEYL
                     YAFLLLSHENTIPLAVGLGHFLASDDSPWEILMATALIYGLPPAAIYYAFRGYMVGGL
                     TSG"
     gene            complement(849..1718)
                     /locus_tag="VNU48_00735"
     CDS             complement(849..1718)
                     /locus_tag="VNU48_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWI09835.1"
                     /translation="MAISATAPASAPPAVRRAPDRWQFWGRMLVAPYMLVFLVFVLYP
                     VGYGLWLARHPSSYVKLFEDPIFFRTVINTIVFLVVAINVKMVIALVLSGFFVQQRWW
                     IKLLSLIFILPWALPSIPTILSVRFMLNPEWGVINTLIFKLTGADGPNWLNDPTLALG
                     FSMLMHVWKSLPFWTLILIAGRLAIPAEQYEAASVDGATNWQKFKFITWPSMRTLYVT
                     SLILSMIWTLGDFNSVYLLTGGGPADLTHVLATIGIRYLRLDQVDLAMASIVVAMPLV
                     LPLVYFMMKRLSK"
     gene            complement(1772..>2612)
                     /locus_tag="VNU48_00740"
     CDS             complement(1772..>2612)
                     /locus_tag="VNU48_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI09836.1"
                     /translation="VQSGYRQKTGTRIYAIGQPMDVDSSDSFYSFLTFMDAYNVKLVN
                     DSGKLLVDDPAVRQGLINALTDYTSVYSKGCSPPSSTSWKDPDNNAAFFNKTTVMTHN
                     ATISIAAKWLDDANNPAFTDEQRATAKKNYAELVRTAGFPSKPDGGKMVYRAAVKTGV
                     VFEQAKNKAGAKQFVSFLLEEANLTPYVEGSLGRWFPVTKTAQQSAFWKADRHRLSVY
                     NQFTSGTVPFEFTKNYKFTVLNNENVWAKAMNRILNEKVPADKAVDEMIARIKTVAGS
                     ATP"
BASE COUNT          476 a          794 c          900 g          442 t
ORIGIN      
        1 cgccggacgt aaggccgccg accatgtagc cgcggaatgc gtagtagatc gccgccgggg
       61 gcaggccgta gatcagcgcg gtggccatca ggatctccca cggcgagtcg tccgaggcga
      121 ggaagtggcc gaggccgacc gcgagcggga tggtgttctc gtgcgagagc agcaggaacg
      181 cgtagagata ctcgttccac gcgagcagca gcgcataggt gaagatcgcg accagcgacg
      241 gcaccatcag cggcaggtag acgagccgga acagttgcag cggcgaggcg ccgtccatgc
      301 gcgcggcctc gtcgagctcc cagggcagct tgtcggacgc ctgcttcaac acccagatgc
      361 agtagggcga ggcgatcgtg accatcgcca ggatcagcga ccactggctg ttcagcaggc
      421 cgtagttgcc catggtcttg tacatcggca cggcgaggaa ggcggccggg atgaagtagg
      481 tgaacagggc caggttcatc accgtgcgcc cgccgcgcac cttgaggcgg ctgatcgcga
      541 acgccgcggc ggtcgagacg aacaaggtga tcgcggcgac cgcgaccgcg atcagcaccg
      601 agttgccgag ctggacccag aagtggtcga ggtagaagtg ctgccggttg aagacgatcg
      661 agaagttctg cagcgtcggc tctttcggcc agaggcgccc cgacgtcgcc gagtccttcg
      721 acgagatcgc gaacaggaac aggtggtaga ccgggatgat ggtccacagg aacaagggga
      781 tgccgatcag cagcagcttg gcctcggccg tgacggcctt ccaggtcttg gccttcatgt
      841 cctgcccttc actttgaaag ccgcttcatc atgaagtaga cgagcggcag caccagcggc
      901 atcgcgacga cgatcgaggc catcgccagg tcgacctggt ccagccgcag gtagcggatg
      961 ccgatggtgg cgagcacatg cgtcaggtcg gccgggccgc cgccggtcag caggtagacg
     1021 ctgttgaagt cgcccagcgt ccagatcatc gacaggatca gcgaggtcac gtagagcgtg
     1081 cgcatcgacg gccaggtgat gaacttgaac ttctgccagt tggtggcgcc gtcgaccgac
     1141 gcggcctcgt actgctcggc gggaatggcc agccgcccgg cgatcaggat cagggtccag
     1201 aacggcagcg acttccagac gtgcatcagc atcgagaagc ccagcgccag ggtcgggtcg
     1261 ttgagccagt tcgggccgtc ggcgccggtg agcttgaaga tcagcgtgtt gatcacgccc
     1321 cactcggggt tcagcatgaa acgcaccgac aggatcgtcg ggatcgacgg cagcgcccac
     1381 ggcaggatga agatcagcga cagcagcttg atccaccagc gctgctgcac gaagaagccc
     1441 gacaacacca gcgcgatgac catcttcacg ttgatcgcga cgaccaggaa gacgatcgtg
     1501 ttgatgacgg tgcggaagaa gatcgggtcc tcgaacagct tgacgtagct ggacgggtgg
     1561 cgcgccagcc acaggccgta gcccaccggg tacagcacga agaccaggaa caccagcatg
     1621 tacggggcca ccagcatgcg gccccagaac tgccagcgat cgggggcgcg gcgcactgcg
     1681 ggcggtgcgg aagcgggtgc ggttgcgctg atggccatgg gtgtgggtgg tcgaggcggc
     1741 ggcgcgggtc ggccgcgccg ccgtcggccg gtcagggtgt cgcgctgccg gccaccgtct
     1801 tgatgcgcgc gatcatctca tcgaccgcct tgtccgccgg caccttctcg ttgaggatgc
     1861 gattcatcgc cttggcccag acgttctcgt tgttcagcac cgtgaacttg tagttcttgg
     1921 tgaattcgaa cggcaccgtg ccgctcgtga actggttgta gaccgagagg cggtggcggt
     1981 cggccttcca gaacgcgctt tgctgtgcgg tcttcgtgac cgggaaccag cggcccagcg
     2041 aaccctcgac gtacggcgtc aggttggcct cctccagcag gaacgagacg aactgcttgg
     2101 ccccggcctt gttcttggcc tgctcgaaca cgacgccggt cttgaccgcg gcgcggtaga
     2161 ccatcttgcc gccgtccggc ttgctcggga agcctgccgt gcgcaccagc tcggcgtagt
     2221 tcttcttcgc ggtggcgcgt tgctcgtcgg tgaaggcggg gttgttggcg tcgtccagcc
     2281 acttcgcggc gatcgagatc gtcgcgttgt gcgtcatcac cgtcgtcttg ttgaagaacg
     2341 cggcgttgtt gtccggatcc ttccagctcg tgctcgacgg cggcgagcag cccttcgagt
     2401 acaccgaggt gtagtcggtg agcgcattga tcagcccctg gcgcaccgcc gggtcgtcga
     2461 ccagcagctt gccgctgtcg ttcaccagct tgacgttgta ggcgtccatg aaggtgagga
     2521 acgaatagaa cgagtcgctg gaatccacgt ccatcggctg gccgatcgcg tagatgcgcg
     2581 tgccggtctt ctggcgatag cccgactgca cc
//
LOCUS       DATMJB010000034        12712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_823271, whole genome shotgun sequence.
ACCESSION   DATMJB010000034 DATMJB010000000
VERSION     DATMJB010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12712
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823271"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..869)
                     /locus_tag="VNU48_00745"
     CDS             complement(87..869)
                     /locus_tag="VNU48_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI09837.1"
                     /translation="MANQAEDFTGQVVLITGGGKGVGRGISLRFLEAGATVVICGRTL
                     PDTLPQAGGRSAAFIMADVRDAEAVDALVSTILEQHGRLDVLVNNAGGTPYVLAAEGS
                     ARFHESMIRLNLLAPLLLAIRANAVMQKQVEGGVVVFIGTVGAFDARPGTATYAAAKA
                     GVVSLARSLAVEWAPRVRVVSVSPGLVMTEQSHQHYGDAAGIAAVSATVPLGRMAEPV
                     DIGDACVFMASRRASYVSGVDLLVHGGGEKPAFLDAANVNKT"
     gene            1042..1866
                     /locus_tag="VNU48_00750"
     CDS             1042..1866
                     /locus_tag="VNU48_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI09838.1"
                     /translation="MPQPIPEQHYVQLPNGLRLHYNCAGGGSRVVVFLHGSGPGASGH
                     SNFKFNYPWLARQGWRCIVPDLPGFGLSSKPADRDYVLDFFVETLHAFLQAIGVTQCV
                     LVGNSLGGAIAMKYTLDHPDNVSHLIVMGPGGLEERETYFRMEGIQRMVSDFAGGALD
                     REGMRGLLKLLVFDPVHITEELLDERVPVVAMQPKEVLATMRVPNLSDRLGEIRVPVL
                     GFWGVNDRFCPASGAMKMLEGCPDVEFVLVNRCGHWVMVEHTEMFNQAVLAFLKRH"
     gene            1875..2666
                     /locus_tag="VNU48_00755"
     CDS             1875..2666
                     /locus_tag="VNU48_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWI09839.1"
                     /translation="MDDSRIDALAAELYAALRERRTLPPLTQREPDITIEDAYRISTT
                     VLDRRMRDGERVVGKKIGVTSKAVQDMLGVFQPDFGFITDRMHYADGATIGLRASGLI
                     QPRAEGEIAFVLRSDLNGPGITMDDVLAATDYVAPCFEIVDSRIADWKIRIQDTVADN
                     ASCGVFVLGTQRLAPRALDLAAVQLDMTRNGQPCGSGLGSAVQGHPAAAVAWLANTLG
                     RFGIPFKKGEIILSGSLAPLVPASPGDHFELTISGMGGASVSFAA"
     gene            2681..3592
                     /locus_tag="VNU48_00760"
     CDS             2681..3592
                     /locus_tag="VNU48_00760"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HWI09840.1"
                     /translation="MKKIKCAVIGPGNIGTDLLYKLRRSPVLEPVWMVGIDASSEGLS
                     RAREFGLKTTSDGVAGLLPHVKADRVQIAFDATSAYVHAENSRKLNELGVLMIDLTPA
                     AIGPFCVPPVNLKAHVGKREMNVNMVTCGGQATIPMVAAVSRVQAVAYGEIVATVSSR
                     SVGPGTRKNIDEFTRTTAGAVEQVGGARKGKAIIIINPAEPPMIMRDTIHCLTELEPD
                     REAITRSVKEMVAEVQKYVPGYRLKNGPVFEGKRVSVFMEVEGLGDYLPKYAGNLDIM
                     TASASRTAEMFAEEILAGRLNLESLIA"
     gene            3610..4641
                     /gene="dmpG"
                     /locus_tag="VNU48_00765"
     CDS             3610..4641
                     /gene="dmpG"
                     /locus_tag="VNU48_00765"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008701 - 4-hydroxy-2-oxovalerate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HWI09841.1"
                     /translation="MNLKGRKITLHDMSLRDGMHPKQHRISLDQMVAVARGLDEAGVP
                     LIEVTHGDGLGGASVNYGFPAHSDEEYLRAVIPQMKRAKVSALLIPGMGTVDHLHLAH
                     DCGVHTIRVATHCTEADVSEQHIGLGRKLDLDTVGFLMMAHMLDGPGLVKQALLMESY
                     GANCLYITDSAGYMLPDDVRERLGAVRQALKPETELGFHGHHNLAMGIANSLAAIEVG
                     ANRIDGSAAGLGAGAGNTPLEVFNAVCNRMGIVTGVDVFKLSDVAEDLVYPMMDHIVR
                     IDRDALALGYAGVYSSFLLFAKRAEAKYKIPARDILVELGRLRMVGGQEDMIEDTAMD
                     MARARGLLQ"
     gene            4676..5824
                     /locus_tag="VNU48_00770"
     CDS             4676..5824
                     /locus_tag="VNU48_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HWI09842.1"
                     /translation="MQATIDPAIEASLLSGRTWDDFCDALKRAGQQVLRPETPANAFD
                     RAEGWRYLSRLTRLGLEMFVEAGTPEFPTFMVPSHETAKIGADNPDMSYMAARISGRY
                     RYRVWGQRGTVASINFSTKRGGYDQGGKLLPGGFIDTNTLQVGPDGQFELTLSSEPAP
                     GNWLKIDPDTVQLLVRQVFLDRRSEKPADLRIERLDAAGETPPPLAPAALHDNLKRAG
                     SFVENTARMFADWAQTFLPHTNQLPPADQAYCQSIGGDPNIFYYHSYWALEDDEALLF
                     ELDRIPPCEYWNLQIDNHWMESLDYRYFRICLNKHSARLRPDGGLRLVLAHRDPGVAN
                     WLQTAGHRRGTMCWRWIGAPNPVHPTTRVVKLIELDKEPHDGRHPSIS"
     gene            5913..6986
                     /locus_tag="VNU48_00775"
     CDS             5913..6986
                     /locus_tag="VNU48_00775"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006497785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWI09843.1"
                     /translation="MLQSIRDEAQMTPAGQLQFAHRVLESLCNRLTLEDYCARHPEIL
                     AERIERPILIVGLPRTGTTMLHRVLARDPRFYTMAWWEARFPSPLSEADAADPTRRID
                     MAKAEVKAMIAAVPEVLAMHPLDAELPDEEVLLMEHAFMSAIDSYGNVPGYMDWLWRQ
                     DQTCAYLYLRKQLQFLQWQKRRRGITAERWLLKAPHHTHLMPTLFKVFPDVKVIQTHR
                     DPLQTIPSMGSFAYTLWRMFSDKADPAQAGRQWSDKFARGMRNAMAFRETMPSDRFMD
                     VWYLDAVAHSMDVVEKVYPFVGMALDDGTRERMRQWMASSSREGRPTHEYSVERMGLS
                     NDQLKQDFAAYRERHVLPHLNAR"
     gene            7041..7520
                     /locus_tag="VNU48_00780"
     CDS             7041..7520
                     /locus_tag="VNU48_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWI09844.1"
                     /translation="MSKAKNIVTSIEVDIAAPASVVWEVLTDLPRYREWNPFTVQIES
                     TLALGDPVDLHIPIPGQAGQEMVVREYLIAFEPDRLLSWEQRPAGDNKDAARRDQYIE
                     SLGPERSRYFTTDIFLGLNADQIMANFGGWVKASFDAVAEGVKKQAEALHAARRAAR"
     gene            7531..8499
                     /locus_tag="VNU48_00785"
     CDS             7531..8499
                     /locus_tag="VNU48_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012467661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HWI09845.1"
                     /translation="MTHAHHYAFNGDADGLCALQQLRLADPAAATLVTGVKRDIQLLE
                     RVVAGRGDHVTVLDISLDSNRDGLMRLLDAGASVRYFDHHHAGTQPVHERFESHIDTA
                     GDVCTSVLVDRHLGGRHRAWAITAAFGDSLPLVGKAMAQASGLGAEATARLERLGIAL
                     NYNAYGEQVCDLHFDPARLADAMLPYVDPASFIAESPVFEQLWAGYESDMAQARSLRP
                     ERQAPGATLLVMPDAAWARRAIGVLANDLMHSLPGSAIAILSPRSGGGYTVSVRVPKE
                     SSTGADEFCRQFPTGGGRRGAAGVNDLPAGEFDRFVARFEASFARS"
     gene            complement(8512..9717)
                     /locus_tag="VNU48_00790"
     CDS             complement(8512..9717)
                     /locus_tag="VNU48_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI09846.1"
                     /translation="MTASTETTSKAGREVGHAQREPAGGVPLSLRQASDLIGTVYQGM
                     IESVPWSSLLWQVRGLLGANWVTLIVRPPSENSQARIINAGPTGVTIAEGHWTSYNIF
                     AIDPMVHLPTGRMVTVEEMIGDTNWLTCEFYKLFVEPHDIRYLMGADICTDDGIDCRF
                     RVCRPASSKPFSDAEKRVGEFLLPHLRRSVELHARLGTLDSERRLFAATVDRMLVGTV
                     ILDAQGAIFTTSRVAQEILAENDGLRLAAGGLQASYAAEDKELQRLIKQAVSGIHSTA
                     QAVGQAMSITRPSGRGRLGIAVRTNPITEWAEGKRWPAVTVFLRDPGRKSQGSVEILR
                     KLYALTPAEALLSMQLVEGLTLDEAADHLNIRKNTARAHLRSIFSKTNVTRQTSLVSL
                     LMSSMGSLE"
     gene            9881..10867
                     /locus_tag="VNU48_00795"
     CDS             9881..10867
                     /locus_tag="VNU48_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HWI09847.1"
                     /translation="MTADRAFDPKEFRKTLGTFTTGVTIITTRAADGTPVGITANSFN
                     SVSLDPPMVLWSLARNARSLAAFDKAQHWAVHILSADQEPLSNRFARSSEDKFSGVPV
                     DTGIGDVPLLSNCTSRLQCRTSFKYEGGDHIIFVGEVLAFDRNDVAPLVFHGGAYAVA
                     ARKTDSSLAGSAPVRDVETSFGEDFMAYLLTRAHFQVYAPLRKLLRSHALSDAEWFVL
                     SSLTAKNGRSFAELNAMYAITGDELPGPVLEVLEKRELLCRSSDAADAAVQLTDAGRD
                     MALRVLAAAKAIEADLTPRLGIHESGALKNLLRQVIQLTDPGLPDMWGRAPG"
     gene            complement(10998..>12712)
                     /locus_tag="VNU48_00800"
     CDS             complement(10998..>12712)
                     /locus_tag="VNU48_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWI09848.1"
                     /translation="GTINTPEFNGLSARVTLGHSQSDGDVKNTAPKQTFAFTGPLGPY
                     SSVTTNERGGDNRTDSGMIAVSYKGVPNLKVDYKFDFSNWKGTLNYRQTGDVVNGVAT
                     GFDYQTSLFVPLEGTAKNDVRGHSLIAEYALNKNLTVKYIGGFRNYDLDVPGNQVFGE
                     AGTTDLGGGNIGTSLFAKRAESQRQSSHELQLIGTVGSVDWLAGLFTFEEKGSANAPV
                     VFGTNYGGAPIGPTNTIDPAFFHYFIGGDIDVKNKSDAAYLHSTWHINNQFDLSGGVR
                     HTRDKRSEHIIAAGLLGATTGSPPVDSEFSGSHTDYDASITYKITPEASVYAKYATGY
                     VSGGTLRGTKFDPEEMKAFEVGFKSELLERRLRLNVALFHQKRKDAQIEFFDGNTGGV
                     LMGKSDTKASGLEVEANAKITRNLSLNGSYGLTEATQSGDLRVTQPKHTLYLGTEYKF
                     DRLGNGIVPSARLDANWHSAYYTMKCPAGTAQDPGKDTCSGTVNAPVDAATKLKSDTL
                     LSARASLSDIKLFGNATGRLSLWGRNLLNKHDPEYNFSLGATTIATTFQRPRTFGVDF
                     AMDF"
BASE COUNT         2226 a         4261 c         4072 g         2153 t
ORIGIN      
        1 gcgataggca cggccgatcg gggtgcgtgc gacggaaacg atgactgctt ctttcatggc
       61 cattccttca tgacgaatac gctggatcaa gtcttgttga cgttggcggc gtcgagaaag
      121 gcgggcttct cgccgccgcc gtgcaccagc aggtccacgc cgctgacata ggaagcgcgc
      181 cgtgacgcca tgaagacgca ggcatcgccg atgtccaccg gctcggccat gcggcccagc
      241 ggcacggtgg ccgagacggc cgcgatgccc gccgcgtcgc cgtagtgctg gtgcgactgc
      301 tcggtcatca ccaacccggg gctgaccgac accacgcgca cccgcggtgc ccattcgacc
      361 gccaacgaac gggccaggct gacgacgccg gcctttgccg cggcgtaggt cgcggtgccg
      421 ggccgcgcat cgaacgcgcc gaccgtgccg atgaagacca ccacgccccc ttcgacctgt
      481 ttctgcatca ccgcgttggc acgaatcgcc agcagcagcg gcgccagcag gttcaggcgg
      541 atcatcgact catgaaagcg cgccgaaccc tcggccgcga gcacataggg cgtgccgccg
      601 gcgttgttga ccagcacgtc gagccgaccg tgttgttcga ggatcgtcga gaccagcgcg
      661 tccaccgcct cggcgtcgcg cacgtcggcc atgatgaacg cggcactgcg gccaccggct
      721 tgcggcagcg tgtcgggcag cgtgcggcca cagatgacga cggtcgcacc cgcctccaga
      781 aagcgcaggc tgatgcctcg gcccacgccc ttgccgccgc cggtgatcag gaccacctgg
      841 ccagtgaaat cctcagcttg gttcgccatg tcgttgcctt ctcctgctgc cgcgaatgct
      901 gggggtgatt cgcgacgcac gcatggtctg aaaggacgat atgaaagggc agaacgctta
      961 gtctgatcag acgatgcctg gcggggcggc caccttgacg attgcgacca cgccgacgcc
     1021 cattcacgcc cggcgcgccg aatgccccaa ccgattccag aacagcacta cgtgcagctt
     1081 ccaaacgggc tgcgcctcca ctacaactgc gccggcggcg gcagccgcgt cgtggtgttt
     1141 ctccacggca gcggccccgg ggccagcggc cacagcaatt tcaagttcaa ctatccgtgg
     1201 ctggctcgcc agggatggcg ctgcatcgtc ccggacctgc cgggtttcgg gctgtcctcg
     1261 aagccggccg atcgcgacta cgtgctcgac ttcttcgtcg agacgctgca cgcgttcctg
     1321 caagccatcg gggtgacgca gtgcgtgctc gtcggcaact cgctcggcgg cgcgatcgcg
     1381 atgaagtaca cgctcgatca ccccgacaac gtcagccacc tgatcgtcat ggggccgggc
     1441 ggcctggaag agcgcgagac ctatttccgc atggaaggca tccagcgcat ggtgtccgac
     1501 ttcgccggcg gcgcgctcga ccgcgaaggc atgcgcggcc tgctgaaact gctggtgttc
     1561 gaccccgtcc acatcaccga agaactgctc gacgagcgcg tgcccgtcgt cgcgatgcag
     1621 ccgaaggaag tgctcgctac gatgcgtgtg cccaacctca gcgatcgtct cggcgagatc
     1681 cgcgtgccgg tgctcggctt ctggggtgtc aacgaccgat tctgtcccgc cagcggcgcc
     1741 atgaagatgc tcgaaggctg ccccgacgtc gagttcgtgc tggtcaatcg ctgcggtcat
     1801 tgggtcatgg tcgagcacac cgagatgttc aaccaggcgg tgctcgcctt tctcaagcgt
     1861 cattgaggca gcccatggac gacagtcgca tcgacgccct cgcggccgag ctttacgccg
     1921 ccctgcgcga acggcgcacc ttgccgccgc tgacgcagcg cgagcccgac atcacgatcg
     1981 aagacgccta ccgaatctcc accaccgtgc tcgaccgccg catgcgcgat ggcgagcgcg
     2041 tcgtcggcaa gaagatcggc gtcacatcga aggcggtcca ggacatgctg ggtgtcttcc
     2101 agcctgattt cggcttcatc accgaccgca tgcattacgc cgacggcgcc acgatcgggc
     2161 tgcgcgcgtc cggcctgatc cagccgcgcg ccgagggcga gattgcgttc gtgctgaggt
     2221 ccgacctcaa tgggcccggc atcacgatgg acgacgttct ggccgccacc gactacgtgg
     2281 cgccgtgctt cgagatcgtc gactcgcgca tcgccgactg gaagatccgg attcaggaca
     2341 cggtcgccga caacgcgtcg tgcggcgtgt tcgtgctcgg cacgcagcgc ctggcccccc
     2401 gtgccctcga cctggccgcc gtacagctcg acatgacgcg caatggccag ccctgcgggt
     2461 ctggcctggg ctcggcggtg cagggccacc cggccgccgc ggtggcctgg ctggcgaaca
     2521 cgctgggccg attcggcatt ccgttcaaga agggcgaaat catcctgtcc ggctcgctcg
     2581 cgccactcgt gcccgccagc ccaggcgatc acttcgaact gaccatcagt ggcatgggcg
     2641 gcgcgtccgt ctcgttcgcc gcatgaatca aggaactgcc atgaagaaga tcaagtgcgc
     2701 cgtgatcggg cccggcaaca tcggcaccga cttgctctac aagctgcggc gcagcccggt
     2761 gctcgagccg gtgtggatgg tcggcatcga cgcaagctcc gagggcctga gccgagcccg
     2821 cgagttcggg ctcaagacga cgtccgacgg cgtggccgga ctgctgccgc acgtgaaggc
     2881 cgaccgcgtg cagatcgcct tcgacgcgac cagcgcctat gtccacgccg aaaacagccg
     2941 caagctgaac gaactgggcg tgctgatgat cgacctgacg ccggcggcca tcggcccttt
     3001 ctgcgtcccg cccgtcaatc tcaaggccca cgtcggcaag cgcgagatga acgtcaacat
     3061 ggtcacctgc ggcggccagg cgaccattcc gatggtcgcc gccgtcagcc gcgttcaggc
     3121 cgtggcctac ggcgagatcg tggccacggt gtcgagccgc tcggtcggcc cgggcacgcg
     3181 caagaacatc gacgaattca cccgcaccac ggcgggcgcg gtcgaacagg tcggcggcgc
     3241 caggaagggc aaggccatca tcatcatcaa cccggccgag ccgccgatga tcatgcgcga
     3301 cacgatccac tgcctgaccg agctggagcc ggatcgcgag gcgatcacgc gctcggtgaa
     3361 ggagatggtg gccgaggtgc agaagtacgt gccgggctac cggctcaaga acggcccggt
     3421 cttcgagggc aagcgcgtgt cggtgttcat ggaggtcgaa ggcctcgggg actacctgcc
     3481 gaagtacgcg ggcaacctcg acatcatgac ggcctccgcg tcccgcaccg ccgagatgtt
     3541 cgccgaggag atcctcgccg ggcggctgaa cctcgaatcg ctgatcgcct gagcgcgctg
     3601 gagagaacga tgaacctcaa aggcaggaag atcaccttgc acgacatgtc gctgcgcgac
     3661 ggcatgcacc ccaaacagca ccgcatcagc ctcgaccaga tggtggcggt ggcgcgcggc
     3721 ctggacgaag ccggggtgcc gctgatcgag gtgacgcacg gcgacgggct cggcggcgcc
     3781 tcggtgaact acggcttccc ggcgcattcc gacgaggaat acctgcgcgc ggtgattccg
     3841 caaatgaagc gcgccaaggt gtcggcgctg ctgatccccg gcatgggcac ggtggaccac
     3901 ctgcacctgg cgcacgactg tggcgtgcac acgatccgcg tggccacgca ttgcaccgag
     3961 gcggacgtgt ccgaacagca catcggcctg ggccgcaagc tcgatcttga cacggtcggg
     4021 ttcctgatga tggcgcacat gctcgacgga ccgggcctgg tcaaacaggc cctgctgatg
     4081 gagagctacg gcgcgaactg cctgtacatc accgattcgg ccggctacat gctgcccgac
     4141 gacgtgcgcg aacgattggg cgccgttcgc caggcattga aacccgagac cgagctcgga
     4201 ttccacggcc accacaacct ggcgatgggg atcgcgaatt cgctcgcggc gatcgaggtc
     4261 ggcgcgaacc gcatcgacgg ctccgccgcc ggcctgggcg ccggcgccgg caacacgccg
     4321 ctggaggtgt tcaacgcggt gtgcaatcgc atgggcatcg tcaccggcgt cgatgtgttc
     4381 aagctctcgg acgtcgccga ggacctggtc tacccgatga tggatcacat cgtgcgcatc
     4441 gatcgcgacg cgctggccct gggctatgcc ggtgtgtact cgtcgttcct gctgttcgcg
     4501 aagcgggccg aagcgaagta caagatcccg gcgcgcgaca tcctcgtcga actcggtcgg
     4561 ctgcgcatgg tcggcgggca ggaggacatg atcgaagaca cggccatgga catggcgcgc
     4621 gcgcgtgggc tcttgcagtg atcgccgcgg ccacgcgtta gtcagacaga ccatcatgca
     4681 agcgaccatc gatccggcca tcgaagccag cctcctgtcg ggtcggacct gggacgactt
     4741 ctgcgacgcg ctcaagcgcg ccggccaaca agtcctgcgc cccgaaacgc ccgcgaatgc
     4801 gttcgaccgg gccgagggct ggcgctacct gagccggctg acacgcctgg gcctggagat
     4861 gttcgtcgag gccggcacgc ccgagttccc gaccttcatg gtgccttcgc acgagaccgc
     4921 caagatcggc gccgacaacc ccgacatgag ctacatggcc gcgcggatca gcgggcgata
     4981 ccgctaccgg gtctggggcc agcgcggcac ggtggcctcg atcaacttca gcaccaagcg
     5041 cggcggatac gaccagggcg gcaagctgct gcccggcggg ttcatcgaca ccaacacgct
     5101 gcaggtcggg ccggacggcc agttcgaact gaccctttcc agcgagcccg cgcccggcaa
     5161 ctggctcaag atcgaccccg acaccgtgca gttgctggtg cgccaggtgt tccttgaccg
     5221 ccgcagtgaa aagcccgcag accttcgcat cgaacgcctc gacgccgcgg gcgaaacgcc
     5281 acccccgcta gcccccgctg cgctgcacga caacctcaag cgcgcaggaa gctttgtcga
     5341 gaacacggcg cgcatgtttg ccgactgggc gcagacattc ctgccccaca ccaatcagtt
     5401 gccacccgcc gaccaggcct actgccagtc gattggcggc gaccccaaca tcttctacta
     5461 ccactcgtac tgggcgctgg aagacgatga agcgctgctg ttcgagctcg accgcatccc
     5521 accgtgcgag tactggaacc tgcagatcga caaccactgg atggagtcgc tggactaccg
     5581 ctacttccgg atctgcctga acaaacactc ggcacgcctg cgacccgacg gcgggctgcg
     5641 cctcgtgctg gcccaccggg accctggcgt tgcgaactgg ctgcaaaccg cagggcaccg
     5701 gcgcggaacg atgtgctggc gctggatcgg cgcgccgaac cccgtgcatc ccaccacccg
     5761 tgtggtgaag ttgatcgaac tcgacaagga gccccatgat ggccggcacc cctctatctc
     5821 ttgattccaa tgccctgatg gcagccgcta cccaacgcac ggggggcctg tccgacttcg
     5881 gcgatccgag ttttcggccg gcgctggacc gcatgctcca gtccatccgc gacgaagcgc
     5941 aaatgacgcc ggccgggcag cttcaatttg cgcaccgcgt gctggaaagc ctgtgcaacc
     6001 gcctgacgct cgaggactac tgcgccaggc acccggagat cctcgccgag cgcatcgagc
     6061 ggccgattct catcgtcggc ctgccgcgca ccggcaccac catgctgcac cgcgtgctgg
     6121 cccgcgaccc gcggttctac acgatggcct ggtgggaggc gcgctttccc tcgccgttgt
     6181 cggaagccga tgcggcggat cccacccgtc gcatcgacat ggccaaggcc gaggtgaagg
     6241 ccatgatcgc cgcggtgccc gaggtgctgg ccatgcaccc gttggacgcc gagctgcccg
     6301 acgaagaggt gctgctgatg gagcacgcct tcatgagcgc catcgactcc tacggcaatg
     6361 tgcccggcta catggattgg ctgtggcggc aagaccagac ctgcgcctac ctctacctgc
     6421 gcaagcagct ccaattcctg cagtggcaaa agcgccgacg cggcatcacc gcggagcgct
     6481 ggctgctgaa ggcgccgcac cacacacacc tgatgcccac gctgttcaag gtgttccccg
     6541 acgtcaaggt gatccagacg caccgcgacc cgttgcagac gattccgtcg atgggcagct
     6601 tcgcctacac gctgtggcgc atgttcagcg acaaggcaga tccggcacag gccggccgcc
     6661 aatggtcgga caagttcgcc cgcggcatgc gcaacgccat ggcatttcgg gagacgatgc
     6721 cgagcgatcg tttcatggat gtctggtacc tcgatgccgt ggcccactcg atggatgtgg
     6781 tcgaaaaggt ctatcccttc gtcggcatgg cgctcgacga cggcacccgc gagcggatgc
     6841 gccagtggat ggccagcagc agccgcgaag gccggccgac ccatgaatat tcggtcgagc
     6901 gcatgggcct cagcaacgac cagttgaaac aagacttcgc ggcctaccgc gagcgccacg
     6961 tgctgccgca cctgaacgcg cgctgacgac atcacagacc ggggcgaagg acaaggtact
     7021 gcgcaccacc aaggacaacc atgagcaaag caaagaacat cgtcacgtcg atcgaggtcg
     7081 atatcgccgc cccggcgtcg gtcgtgtggg aggtgctgac cgacttgccg cgctaccgcg
     7141 agtggaaccc gttcacggtc cagatcgagt cgacgctcgc gcttggcgac cctgttgacc
     7201 tgcacatccc gatcccgggc caggcgggcc aggagatggt ggtgcgcgag tacctgatcg
     7261 ccttcgagcc ggaccgcctg ctgtcgtggg agcagcgccc ggccggcgac aacaaggacg
     7321 ccgcgcgccg cgaccagtac atcgagtcgc tcggccccga gcgcagccgc tatttcacga
     7381 ccgacatttt tctcggcctg aacgccgacc agatcatggc caacttcggc ggctgggtga
     7441 aagccagctt cgatgcggtg gccgaaggcg tgaagaagca ggccgaggcg ctgcacgccg
     7501 cgcgtcgcgc ggcccgatag ggatcacccg atgactcacg ctcaccacta cgccttcaac
     7561 ggcgacgccg acggcctgtg cgcgttgcag cagcttcggc tcgccgatcc ggcggccgcg
     7621 acgctcgtga ccggggtcaa gcgcgacatc cagttgctcg agcgcgtcgt tgccgggcgc
     7681 ggcgaccacg tgacggtgct cgacatctcg ctcgacagca atcgcgacgg cctgatgcgg
     7741 ctgctggacg cgggcgccag cgtgcgctac ttcgatcacc accatgccgg cacccagccc
     7801 gtgcacgagc gcttcgaatc gcacatcgac accgcgggcg atgtctgcac cagcgtgttg
     7861 gtcgatcgtc atctgggtgg gcggcaccgg gcctgggcca tcaccgccgc cttcggcgac
     7921 agcctgcccc tggtcgggaa ggcgatggcg caggccagcg gcctcggcgc cgaggcgacc
     7981 gcgcgcctgg agcgactcgg cattgcactc aactacaacg cctacggtga gcaggtgtgc
     8041 gatctgcact tcgacccggc gcgcctggcc gatgcgatgt tgccctacgt cgacccggcc
     8101 tccttcatcg ccgagtcgcc cgtgttcgaa cagctgtggg cgggctacga gagcgacatg
     8161 gcccaggcgc ggtcgcttcg gccggaacgt caggcgcccg gcgccacctt gctcgtgatg
     8221 cccgacgccg cgtgggcgcg gcgcgcgatc ggcgtgctgg ccaacgacct catgcacagc
     8281 cttcccggca gtgccattgc gattctgtca ccgcgttcgg gcggcggcta caccgtcagc
     8341 gtgcgcgtgc ccaaggagtc gagcaccggt gcagatgaat tctgccgaca attcccgacc
     8401 gggggcggcc gccgcggcgc ggccggcgtc aacgacttgc cggcgggcga attcgaccgc
     8461 ttcgtggcgc gtttcgaagc aagcttcgcg cgcagctgac ggccggccac gtcactccag
     8521 cgaccccatg ctgctcatca acaggctcac cagcgacgtc tgacgtgtca cgttggtctt
     8581 cgagaagatc gatcgaaggt gcgctcgagc ggtattcttt cgaatgttca agtggtctgc
     8641 ggcctcgtca agcgtcaagc cttcgacgag ctgcatcgac agcagcgctt cggcgggtgt
     8701 cagcgcgtac agcttgcgca ggatctccac cgagccttgt gacttgcgac ccgggtcgcg
     8761 aaggaacacc gtcacggccg gccagcgctt gccttcggcc cattcggtga tcgggttggt
     8821 tcgcacggcg atgcccagcc ggcctcgccc ggagggccgc gtaatcgaca tggcctgccc
     8881 taccgcctgg gcagtgctgt ggatgcccga caccgcctgc ttgatcaagc gctgaagttc
     8941 cttgtcttcg gcggcatagc tggcctgcag accgcccgca gcgagtcgca ggccatcgtt
     9001 ctcggccagg atctcctgcg ccacgcgact cgtcgtgaag atggcacctt gtgcgtcgag
     9061 aatcaccgtt ccgaccagca tgcgatcgac ggtggcggca aacaaccgac gctcggagtc
     9121 gagcgttccc agtcgggcat gaagttccac cgaacgacgc aggtgcggca acaggaattc
     9181 accgacgcgt ttttcggcat cgctgaaggg cttgctcgat gccggccgac agacgcggaa
     9241 acgacagtcg atgccgtcgt cggtgcagat gtcggcaccc atcaggtagc gaatgtcgtg
     9301 cggctccacg aagagtttgt agaactcgca ggtcagccag ttggtatcgc cgatcatctc
     9361 ctcgacggtt accattcggc ctgtcgggag atgcaccatc ggatcgatgg cgaagatgtt
     9421 gtaggaggtc cagtgacctt cggcgatggt gacgccggtc ggacctgcgt tgatgattcg
     9481 tgcctgcgag ttctcggacg gcggtcgcac gatcagggtg acccagttgg ccccgagcag
     9541 gccgcgcacc tgccataaca ggctcgacca cggaacactc tcgatcatgc cttggtacac
     9601 ggtgccgatg agatcgctgg cctggcgcaa cgacagcgga acgccaccgg ccggctcgcg
     9661 ctgcgcgtgg ccgacttctc ttccagcctt gcttgttgtc tccgttgatg ccgtcatagc
     9721 cctgtaccgg tgctgacaat ctgtgcatca ttctacgact cgctccctgg cggcggttag
     9781 tccccctgga cgatgcccgt tccccggtga acccggaagc tgttcaccac gctccgcgat
     9841 ccgttcgcca tcgcgagccc atgcccagga aggagcaagc atgaccgctg atcgcgcatt
     9901 cgaccccaag gagtttcgca agacactggg aacattcacg accggggtga ccatcatcac
     9961 gacgcgcgcc gccgacggca ccccggtggg catcaccgca aacagcttca actcggtctc
    10021 actcgacccg ccgatggtct tgtggagcct ggcgcgcaac gcccgaagcc tcgcggcctt
    10081 cgacaaggcg cagcactggg cggtccacat cctgtcggcc gaccaggagc ccctgtcgaa
    10141 tcggttcgct cgcagcagcg aggacaaatt ttccggcgtg cctgtggaca ccggcatcgg
    10201 tgatgtgccg cttctcagca actgcacgtc aaggctgcaa tgtcgaactt cgttcaagta
    10261 cgagggcggt gaccacatca tcttcgtcgg cgaagtgttg gctttcgatc gcaacgacgt
    10321 ggctccgctg gtgtttcacg gcggcgccta cgccgtggca gcgcgcaaga ccgactcgag
    10381 cctggccggc agcgcacccg tgcgtgacgt ggagaccagc ttcggcgagg acttcatggc
    10441 ctacctgctc acgcgcgcgc acttccaggt ctacgcgccg ctgcgcaagc tgctgcgcag
    10501 ccacgcgctg agcgacgcgg agtggttcgt cctgagcagc ctgaccgcga agaacgggcg
    10561 gtcgttcgcc gagctgaatg cgatgtacgc catcaccggc gacgagctcc cgggccccgt
    10621 gctcgaagtt ctggaaaagc gcgaattgtt gtgccgcagc agcgacgccg ccgatgccgc
    10681 cgtgcagctg accgacgcgg gacgcgacat ggccctgcgc gtgttggcgg ccgccaaggc
    10741 gatcgaggcc gacctgaccc cgcgtctggg gattcacgaa tcgggcgcgt tgaagaacct
    10801 gctgcgccag gtgatccagc tcaccgaccc gggcctgccc gacatgtggg gccgcgcacc
    10861 ggggtagaga gggttcgatt cggcaggccg cgcctgcttc acctccgcgg acacccaaaa
    10921 gacacgggcg actggttcac caccagtcgc ccgtgtcgat tcatgtcagc cgccgcgctc
    10981 gcggcggctg agacggatca gaagtccatc gcgaagtcca cgccgaaggt gcggggccgc
    11041 tggaacgtgg tcgcgatcgt cgttgcgccc agggagaagt tgtactccgg gtcatgcttg
    11101 ttcagcagat ttcgtcccca cagcgacaag cggccggtgg cgttgccgaa cagcttgatg
    11161 tcggacaggg acgcccgcgc gctcaacagc gtgtcggact tcagtttcgt cgccgcatcg
    11221 acgggggcgt tgacggttcc agaacaggta tcttttcccg gatcctgcgc agttccggcc
    11281 ggacacttca tcgtgtagta ggccgaatgc caattcgcat cgaggcgtgc gctcggcacg
    11341 ataccgttgc ccaagcggtc gaacttgtat tcggtgccga gatacagggt gtgcttgggc
    11401 tgagtgacac gcagatcacc cgactgggtc gcttcggtca ggccgtacga accgttcagc
    11461 gacagattcc tggtgatctt cgcgttggct tcgacttcaa gcccgctcgc cttcgtatcg
    11521 ctcttgccca tcaaaacgcc gccggtgttg ccgtcgaaga attcgatctg ggcgtccttc
    11581 cgcttctgat gaaacagggc cacgttgagc cgcaatcgac gctcaagcag ctccgacttg
    11641 aagcccactt cgaatgcctt catctcttcc ggatcgaact tggttccgcg cagtgttccg
    11701 ccggagacat agccggtcgc gtatttcgcg tagacgctgg cttcaggcgt gatcttgtag
    11761 gtgatcgacg cgtcgtagtc ggtgtggctt cccgagaact ccgagtccac cggtggactt
    11821 ccggttgtag cgccgagcaa gccggcggcg atgatgtgct cgctgcgctt gtcacgcgta
    11881 tgacgcacgc caccgctcag gtcgaactgg ttgttgatat gccaggtcga gtgaagatag
    11941 gccgcgtccg acttgttctt gacgtcgata tcgccaccaa taaaatagtg gaagaacgcc
    12001 gggtcgattg tgttggtcgg cccgatgggt gcgccaccgt agttggttcc gaacacaacc
    12061 ggggcgttgg cgctgccttt ttcctcgaag gtgaagagac ccgcgagcca gtccacgctg
    12121 ccgacggtgc cgatcagctg caattcgtgg gagctctgcc gctgcgactc ggcacgcttg
    12181 gcgaacagcg aggtgccgat attgccgcct ccgaggtcgg tcgttccggc ctcgccgaat
    12241 acctggttgc ccggcacgtc gaggtcatag ttgcggaagc cgccgatgta cttgaccgtc
    12301 aggttcttgt tcagcgcgta ctcggcaatg agtgagtggc cgcggacgtc attcttcgcc
    12361 gtgccttcga ggggtacaaa cagcgaagtc tggtagtcaa agccggtggc cacgccgttc
    12421 acgacgtcgc ccgtctgacg atagttcaac gtccccttcc agttcgagaa gtcgaacttg
    12481 tagtcgacct tgagattcgg cacgccctta tagctgacgg cgatcatccc ggagtcggtc
    12541 ctgttgtcgc cgccgcgctc gttggtcgtc acggaactgt acggcccgag cggaccggtg
    12601 aacgcgaagg tctgcttggg cgcggtgttc ttgacgtcgc catcgctctg ggaatggccg
    12661 agtgtgacgc gcgcggacag cccgttgaac tccggcgtgt tgatcgtgcc ct
//
LOCUS       DATMJB010000035         3965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_704209, whole genome shotgun sequence.
ACCESSION   DATMJB010000035 DATMJB010000000
VERSION     DATMJB010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704209"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..989
                     /gene="dapF"
                     /locus_tag="VNU48_00805"
     CDS             117..989
                     /gene="dapF"
                     /locus_tag="VNU48_00805"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HWI09849.1"
                     /translation="MSALANHAFAKMNGIGNEIVVVDLRDKPAAVTPEDARAVASPAG
                     VHYDQLMVLQPPRLRGSEAFIRIYNNDGSEAGACGNGMRCVVRRLFEKTGQTSVTFET
                     QAGLLNCWQGPSADLYTVDMGPPKFGWKDIPLAEEFRDTRYIELQVGPIDAPVLHSPS
                     VVSMGNPHAIFWVDDVNGYDLARFGPMLENHPIFPERANITLAHIVDRDHITIRTWER
                     GAGLTQACGSAACATAVAAARLKRANRTVNIALPGGDLAIEWRERDDHVLMTGTATFE
                     YEGQFDPALFANVA"
     gene            991..2259
                     /gene="mtaB"
                     /locus_tag="VNU48_00810"
     CDS             991..2259
                     /gene="mtaB"
                     /locus_tag="VNU48_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HWI09850.1"
                     /translation="MSVDIVTFGCRLNAFESEVMLREAESAGLTDTIVINSCAVTNEA
                     VAQARQSIRRLKRERPTARIVVTGCAAQTQAQMFAGMAEVDRVVGNDDKMRREAWQAT
                     HAAFEAAPFGIADSEKIAVADIMAVKEMAPHLLEGFQKGLPRVFVQVQNGCDHRCTFC
                     IIPYGRGNSRSVPMGAVVDQVRALTERGHAEIVLTGVDLTSYGADLPGTPKLGQLTKQ
                     ILRHVPELKRLRISSIDSVEADRDLLDVIAGDERLMPHLHLSLQSGDDMILKRMKRRH
                     SRADAIEFCAQLRRLRPDIAFGADIIAGFPTETEEMFERSQELVDECDLTFLHVFPYS
                     PRPGTPAARMPQVNGNAIKQRAKRLRATGEVALQKRLTSEAGTVRAILIESATQGRTE
                     HFMPVAISGKTPGTVLSDRIAGNDGTRLTV"
     gene            complement(2314..3012)
                     /locus_tag="VNU48_00815"
     CDS             complement(2314..3012)
                     /locus_tag="VNU48_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HWI09851.1"
                     /translation="MDVPELTAEEIQARVLHRDGLMLVIDKPAGMPVHRGPKGGANLE
                     SSFDALRFGLPRPPVLAHRLDRDTSGCLVLGRHRKATASLGLLFKHGKISKTYWTVVE
                     GGPAEDEGVIDMPLGRLNAERGWWQKPDPEGQKAVTKWKVMGRGDGLCWLAMEPVTGR
                     THQLRVHSASSGWPIVGDNIYGNGPRFGEPTLHLHSREIGIPISRNKDPVRVIAPAPP
                     HLHERLRACGWNGE"
     gene            3065..>3965
                     /gene="ftsY"
                     /locus_tag="VNU48_00820"
     CDS             3065..>3965
                     /gene="ftsY"
                     /locus_tag="VNU48_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWI09852.1"
                     /translation="MNDTTPGTTKLSWWRRLSSGLKRTSNSLGSAVADLVTKRKLDRA
                     MLEEIEDVLLRADLGTEVAGRISAAVGHGRYDKTISADDVKTVVATEVEKVLAPVAKP
                     LEIDASQKPFVILVVGVNGSGKTTTIGKLAAKLSSEGRKVMLAAGDTFRAAAIEQLKI
                     WGERTKSPVIAGAQGSDSASLAFNALTAAREGQLDVLLIDTAGRLQNKAELMNELEKV
                     VRVIKKVDASAPHAVLLVLDATVGQNALSQVDAFLKTAGVTGLVMTKLDGTARGGILV
                     ALSAKYALPVHFIGVGEGIDDLAP"
BASE COUNT          723 a         1253 c         1312 g          677 t
ORIGIN      
        1 ctcgcgttcg tcccatgaca gatgcgtcat gtggcttccc gctgcggtcg gggctgaccg
       61 cactggccat tccgcggtca ttcgccatat aaggcaggca caaattggga aatttgatga
      121 gcgcgctcgc caaccacgca ttcgccaaga tgaacggtat cggcaacgaa atcgtcgtgg
      181 tcgatctgcg cgacaagccg gccgcggtca ccccagagga cgcgagggcg gtggcctcgc
      241 cggcgggcgt gcattacgac cagttgatgg tgctgcagcc gccgcgcctg cggggcagcg
      301 aagccttcat ccgcatctac aacaatgacg gttcggaagc cggcgcctgc ggcaacggca
      361 tgcgctgcgt ggtacgccgg ctgttcgaaa agaccggcca gacgtcagtg acgttcgaga
      421 cccaggccgg gctgctgaac tgctggcagg gtccgtccgc cgatctctac acggtggata
      481 tggggccgcc aaagttcggc tggaaggata ttcctctcgc cgaagaattt cgcgacaccc
      541 gctacatcga attgcaggtc ggaccgatcg atgcgccggt gctgcattcg ccgtcggtgg
      601 tcagcatggg caatccgcac gcgatcttct gggtcgatga cgtcaacggc tacgacctcg
      661 cccgcttcgg gccgatgctg gaaaatcatc cgatctttcc ggagcgcgcc aatatcacgc
      721 tggcgcacat cgtcgatcgc gatcacatca cgatccgcac ctgggaacgc ggcgcggggc
      781 tgacgcaggc ctgcggctcg gccgcctgcg ccacggcggt ggcggcggcg cggctgaagc
      841 gcgccaaccg tactgtcaac atcgcgctgc ccggcggcga cctcgcgatc gaatggcgcg
      901 agcgcgacga tcacgtgctg atgacgggca cggcgacatt cgagtatgaa ggccagttcg
      961 atcccgcatt gtttgccaac gtcgcgtgag atgagcgtcg atatcgtcac cttcggctgc
     1021 cgcctcaacg ccttcgaatc cgaagtgatg ctgcgcgagg cggaaagcgc cgggctcacc
     1081 gataccatcg tcatcaacag ttgcgccgtc accaacgagg cggtggcgca ggcgcggcag
     1141 tcgatccgcc gcctcaagcg cgagcggcct accgcgcgca tcgtcgtcac cggctgcgcg
     1201 gcgcaaacgc aagcccagat gttcgcaggc atggccgagg tcgatcgcgt cgtcggtaat
     1261 gacgacaaga tgcgacgcga agcgtggcag gccacccatg cggccttcga ggccgcgccc
     1321 ttcggcatcg ccgacagcga aaagatcgcc gtcgccgaca tcatggcggt caaggagatg
     1381 gcgccgcatc tgctcgaagg gtttcagaag ggtctgccgc gggtgttcgt gcaggtgcag
     1441 aacggctgcg atcatcgctg cacgttttgc atcatcccgt atggacgcgg caattcgcgc
     1501 tcggtgccga tgggcgcggt ggtcgatcag gtccgcgcgc tcaccgaacg cggccacgcc
     1561 gagatcgtac tgaccggtgt cgatctcacc agctacggcg cggatctgcc ggggacgccg
     1621 aagctcggtc aattgacgaa gcaaatcctg cggcatgtgc cggaactgaa acggctgcgg
     1681 atttcgtcga tcgactccgt cgaggccgac cgcgatctgc tcgacgtgat cgcgggtgac
     1741 gaacgcctga tgccgcatct gcatctgtcg ctgcaatccg gcgacgatat gatcctgaag
     1801 cggatgaagc ggcgtcattc ccgggccgac gcgatcgagt tctgcgcaca gctgcggcgg
     1861 ctgcggccgg atatcgcgtt cggcgccgat atcatcgcgg gtttcccgac cgagaccgag
     1921 gagatgttcg agcgttcgca ggaactggtg gacgaatgcg atcttacctt cctgcatgta
     1981 ttcccctatt cgccgcgccc gggcacgccg gcggcgcgga tgccgcaggt caacggcaat
     2041 gcgatcaaac aacgtgcgaa gcggctgcgg gcgacgggcg aggtcgcctt gcaaaagcgg
     2101 ctgacgtcgg aggccggcac ggtgcgcgcg attctgatcg agagtgccac acagggccgc
     2161 accgagcact ttatgccggt tgcgatcagc ggcaaaacgc ccggtacggt gctgtcggac
     2221 cggattgccg ggaatgacgg gacgcggctg accgtgtgac ctcaacccgt cgtcgcccgg
     2281 gatggcgatg gatggctgcc gcgctcttgg cgcttactcc ccgttccagc cgcacgcccg
     2341 cagccgttcg tgcaaatgcg gcggcgccgg cgcgatcacc cgcactgggt ccttgttcct
     2401 ggaaatcgga atcccgatct cccgcgaatg cagatgcagg gtcggctcgc cgaagcgcgg
     2461 accgttgcca tagatattgt cgccgacgat cggccagccc gacgaagcgc tgtgcacgcg
     2521 caattgatgg gtgcgacccg tgaccggctc catcgcaagc cagcaaaggc cgtcgccgcg
     2581 tcccatcact ttccatttgg tgaccgcctt ctgtccctcc gggtcgggct tctgccacca
     2641 gccgcgctcg gcgttgagac ggccgagcgg catgtcgatc acgccctcgt cctcggccgg
     2701 gccgccctcg accaccgtcc aataggtctt ggaaatcttg ccatgcttga acaacaggcc
     2761 gagcgaggcc gtcgccttgc gatggcgtcc cagcacgaga cagcccgaag tgtcgcgatc
     2821 gaggcgatgg gcgagcactg gcggccgcgg caacccgaat cgcagcgcgt cgaaggagga
     2881 ttccagattg gcgcccccct tggggccccg atgcaccggc atccccgcgg gcttgtcgat
     2941 gaccagcata agcccgtcac gatggagcac gcgggcctgg atttcttcgg ccgtgagttc
     3001 gggaacatcc atctggcggt cgagactttc gtttgcagga tgaaacggct aacacagcgc
     3061 caacatgaac gataccactc ccggaacgac caaattgagc tggtggcggc ggctttccag
     3121 cggcctgaag cgcacctcga actcgcttgg gtcggcggtg gccgatctcg tcaccaagcg
     3181 gaagctcgat cgcgccatgc tcgaggaaat cgaggacgtg ttgctgcggg ccgatctcgg
     3241 aaccgaagtg gcggggcgga tttccgccgc ggtcggccac ggtcgctacg acaagacgat
     3301 ctcggccgac gacgtcaaga ccgtggtcgc gaccgaagtg gaaaaggtgt tggcgccagt
     3361 ggcgaagccg ctggagatcg acgcctcgca aaagccgttc gtcattctcg tggtcggcgt
     3421 caacggttcc ggcaagacca ccaccatcgg caagctggcc gcgaaattga gcagtgaagg
     3481 ccgcaaggtg atgctggcgg ccggcgacac ctttcgcgcg gcggcgatcg agcaactcaa
     3541 gatctggggc gagcgcacca aatcgcctgt gatcgcgggt gcgcagggtt ccgattcggc
     3601 gagccttgcc ttcaacgcgc tgacggcggc acgcgaaggc caactcgacg tgctgctgat
     3661 cgatactgcc ggacggttgc agaacaaggc cgagttgatg aacgaactcg aaaaggtggt
     3721 tcgcgtcatc aagaaggtcg atgcgtcggc gccgcatgcg gtgctgctgg tgctcgacgc
     3781 caccgttggc cagaacgcgt tgtcgcaggt ggatgcattc ctgaagacgg cgggcgtcac
     3841 cgggctcgtc atgaccaagc ttgacggcac ggcacggggc ggcattctgg tggcgctgtc
     3901 ggcgaaatat gcgttgccgg tgcacttcat cggcgtcggc gaagggatcg acgatctcgc
     3961 gccgt
//
LOCUS       DATMJB010000036         8832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_766426, whole genome shotgun sequence.
ACCESSION   DATMJB010000036 DATMJB010000000
VERSION     DATMJB010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8832
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766426"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..927)
                     /locus_tag="VNU48_00825"
     CDS             complement(172..927)
                     /locus_tag="VNU48_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWI09853.1"
                     /translation="MSIKTATINGVATIEIARPEKKNAITGAMYQQMADALDAAMADG
                     AVRAVLITGQPGIFTSGNDIEDFMQHAPGESPAFGFMRALMGFNKPVIAAVTGAAIGI
                     GTTMLLHCDFVYISDEARLAMPFVSLGLVPEFASSLIVPQLMGNARAAEKILLGDPFT
                     GADAVEVGIANAVLPANEVVNHARRIAERFNALPPGAVRDTKKLMRRARSQAALETIG
                     VEGKVFAERLQSPEAKEAFSAFFQKRKPDFSKF"
     gene            complement(962..2191)
                     /locus_tag="VNU48_00830"
     CDS             complement(962..2191)
                     /locus_tag="VNU48_00830"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWI09854.1"
                     /translation="MSNKQVQEAYIVAATRTPIGRSGRGYFKNTRPDDLLVAAIRSAM
                     LQVPSLDPTAIEDAIIGCSFPEGEQGMNMARIAVALAFPNPIGGVTVNRFCASGITAI
                     QMAADRIRVGEADVMIAGGAESMSLVPIGGGKPSVNPAIFERDENIGIAYGMGITAEK
                     VAVQWKISREAQDAFALESHLRAMKAQAAGEFSDEITPIEVVSRFPNLESGDQGVKTR
                     TVSLDEGPRPDTSLEGLAKLKPVFAAGRDPAGKPTGMGSVTAGNSSQTSDGAGALILA
                     SEKAIKQYGLTPLARFVSFAARGVPPEIMGIGPIEAIPAALRYANLKLDDIGWIELNE
                     AFAAQSLAVIGSLGLDRAKVNPMGGAIALGHPLGATGAIRAATVVHALRRHNLKYGMV
                     TMCIGTGQGAAGIFERV"
     gene            complement(2209..4617)
                     /locus_tag="VNU48_00835"
     CDS             complement(2209..4617)
                     /locus_tag="VNU48_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWI09855.1"
                     /translation="MNRFPVRKVAVLGAGVMGAQIAAHLVNCKVPVILFDLPAKEGPK
                     NGIVTKAVAGLGKLKPAPLGVAEDAALIQPANYEEHLDLLKDCDLIIEAIAERMDWKL
                     DLYKRIAPAVAKHAIVASNTSGLSITKLSEVLPDALKPRFCGIHFFNPPRYMALVELI
                     NTPTTQPEILDQLEAFVTTALGKSVVRAMDTPNFIANRVGIAGMLATMKEAETFGLSY
                     DVVDDLTGKKLGRASSGTFRTADVVGLDTMSHVIKTLQDNLKDDPFYSSYATPPVLAR
                     LIEQGALGQKSGAGFYKRVGKDIQRLDPASGSYVAGGGKADEIVARILKKAPAERLKL
                     LRESKNPQAAFLWAILRDSFHYAAVHLADIAESARDVDFAMRWGFGVSEGPFELWQAA
                     GWTTVANWIKEDIAAGKALSSAPLPAWVFDGPPAAHGGVHRAEGSWSAAHSRWVAPST
                     LPVYARQHFRESVFGSGAGSALTSGTEVFKNDEMRVWTLDGEVLIASITSKLHLIGQG
                     VVEGLTQAVELAERGYKGLVIWSPDDVFSAGANLEQLMPVFMKSGGKGILPEIKKLQD
                     AMLRLRYATVPVVAAVRGVALGGGCEIAVYAAKRVAAMESYMGLVEVGVGLIPGGGGL
                     TYIARRAAEMAAGSSSPGSATADIFKFLSDGFTNAAMAKVGTSASESRKLGYLLWSDV
                     VVPNKDELLYVASQQAKAMFESGYRAPARTLFPVAGRNAIATIKGQLANMRDGAFVSK
                     HDFHLSALIADVICGGDVDAGSLVSEEYLMALERKHFCALLDHEKTQERITGMLQTGK
                     PVRN"
     gene            complement(4763..6553)
                     /locus_tag="VNU48_00840"
     CDS             complement(4763..6553)
                     /locus_tag="VNU48_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HWI09856.1"
                     /translation="MPQYTPPLRDMQFVMHELLNVVDELKALPAHADIDVDTINAVVE
                     EGGKFAATVLAPLNRIGDEQGCVLDKTTHEVTAPKGFKQAYAQYVEGGWPALSCDPAY
                     GGQGLPHLVNQCMFEMMNSANQAWTMYPGLSHGAYEALRAHGSDAQKKTYLPKLTSGE
                     WTGTMCLTEAHCGTDLGMLRTKAEPQTDGTYKLTGNKIFISAGEHDLAANIIHLVLAR
                     LPDAPAGSKGISLFVVPKFLVDADGRLGARNPIFCGGLEHKMGIHGNATAQINLDGAV
                     GTLVGEPNKGLAAMFVMMNAARLGVGMQGLGLTEVAYQNAAAYAKERIQMRSLSGPKA
                     PDKPADPIIVHPDVRKMLLTARAYAEGGRALAMWTTLQLDKELASDDPAVRAECADLV
                     ALVTPIVKAFLTDNAWLSTSHCMQVFGGHGYIAESGMEQFVRDARINMIYEGTNTIQS
                     LDLLGRKVLGDNGAKLKKFGKLVARFVEDEGVNEDMQEFVNPLADLGDKVTKLTTEIG
                     MKAMKNPDEAGAAAVDYLRVCGHLVFAYFWARMAKVALAKVGGGDPFYKAKLTTARFY
                     FAKLLPETATLIRTARAGAAPLMAMDEALF"
     gene            complement(6576..7220)
                     /locus_tag="VNU48_00845"
     CDS             complement(6576..7220)
                     /locus_tag="VNU48_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWI09857.1"
                     /translation="MKGRQTRAAILDAALALASQIGLEGLSIGALAEVTQMSKSGVFA
                     HFGSREELQISVVREYHAKFSDEIFEPAMREPRGLPRLRALYDRWFKRVSIEIDAGCI
                     YISGAVEFDDRPGPVRDALVSMVQTWQNALERAIRIAVDEGHLRADTDPQQMLFEIHG
                     LILALHHDARFLRHSGAEARARTGFENVLSHYLAAPSLPASPVAARGRRGPVST"
     gene            7522..8514
                     /locus_tag="VNU48_00850"
     CDS             7522..8514
                     /locus_tag="VNU48_00850"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWI09858.1"
                     /translation="MKIAVVGLGAVGGLIAARLAMAEFEVCALTRGATLAAVRAHGLR
                     LVAGGEEKVARIAASDDARTLGPQDLVIVALKGPALAAAAASLQPLLGSQTIVMPAMN
                     GVPWWFLDAAPADAALGDRHLASIDPDGRIGAALPLARVLGCVVHLTCSSPEPGLVRH
                     GFGDRLIVGEPGGGPSARVDMVRAALGHAGFQAEASTDIRQAIWYKLWGNMTMNPVSA
                     LTGAESHHIIDDPLVLAFMLGTMEEARRIGARIGCVIEQSGEERMAVARELGAFKTSM
                     LQDCLAGRPLELDALVGAVHEIGTRLGVAMPNIGALLGLTRLMARERGLYPPAA"
     gene            8600..>8832
                     /locus_tag="VNU48_00855"
     CDS             8600..>8832
                     /locus_tag="VNU48_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWI09859.1"
                     /translation="MEVLQLDVSGRPQAWISAKEAAILYASDGVAWTLGDTFYVLRGG
                     YQRTGLQSRIEVHPIIAVRGSVPSRAWRQTPALS"
BASE COUNT         1258 a         3008 c         3057 g         1509 t
ORIGIN      
        1 ccggcgatca gcacgcgcag cgcgcccggc ccgtggccaa tctgcccttc gcgcggaccg
       61 gccgcctcag gcagcaccgg cgcacggcgc cgtgtgctga tcgcctgcgc cgccagcagc
      121 ggcgagagga ccagcttcag cgcgaacgac atggccgcct cccggtcgtg gtcagaactt
      181 cgagaagtcc ggcttgcgct tctggaagaa cgcgctgaac gcctccttcg cctcgggact
      241 ctgcagccgc tccgcgaaga ccttgccttc gacgccgatg gtttcgagcg ccgcttgcga
      301 gcgcgcgcgg cgcatcagct tcttggtgtc gcgcacagca cccggcggca gcgcattgaa
      361 acgctcggca atacggcgtg cgtggttgac gacctcgttg gccggcagca ccgcgttcgc
      421 gatcccgacc tccaccgcat cggccccggt gaacgggtcg ccgagcaaga tcttctcggc
      481 tgcgcgcgcg ttgcccatca gctgcggaac gatcaggctc gacgcaaatt cgggcaccag
      541 gccgaggctg acgaacggca tcgcgagccg cgcctcgtcg gagatgtaaa cgaagtcgca
      601 gtgcagcagc atcgtcgtgc cgatgccgat cgccgcgccg gtgacggccg cgatcaccgg
      661 cttgttgaag cccatcagcg cgcgcatgaa cccgaacgcc ggcgactcgc cgggcgcgtg
      721 ttgcatgaag tcctcgatgt cgttgcccga agtgaagatg ccgggctggc cggtgatcag
      781 cacggcgcgc accgcgccgt cggccatggc cgcgtcgagc gcgtctgcca tctgctggta
      841 catcgcgccg gtgatcgcgt tcttcttctc ggggcgcgcg atctcgatcg tggccacgcc
      901 gttgatggtg gcggtcttga tgctcatggg aaaggtctcc ttttcgatga atggtggtgg
      961 ttcagacgcg ctcgaagatg ccggccgcgc cctggccggt gccgatgcac atcgtcacca
     1021 tgccgtactt caggttgtgg cggcgcagcg catgcacgac ggtggcggcg cggatcgcgc
     1081 cggtcgcgcc cagcgggtgg ccgagcgcga tcgcgccacc catcgggttg accttcgccc
     1141 ggtccaggcc gaggctgccg atgacggcga gcgactgcgc cgcgaaggcc tcgttcaact
     1201 cgatccagcc gatgtcgtcg agcttcaggt tggcatagcg caacgccgcg ggaatcgcct
     1261 cgatcgggcc gatgcccatg atctcgggcg gcaccccgcg cgcggcgaag ctgacgaagc
     1321 gagccagcgg cgtcaggccg tactgcttga tcgccttctc gctcgcgagg atcagcgcac
     1381 ccgcgccatc actcgtctgc gaactgttgc cggcggtgac gctgcccatt ccggtcggct
     1441 tgccggccgg gtcccggcca gcggcgaaca ccggcttcag cttggcgagg ccttccaggg
     1501 acgtgtcggg gcgcgggcct tcgtcgaggc tgacggtgcg cgtcttcacg ccctggtcgc
     1561 cagactcgag gttcgggaag cgcgagacga cctcgatcgg cgtgatctcg tcgctgaatt
     1621 cgccggccgc ctgcgccttc atcgcgcgca ggtgcgactc gagcgcgaac gcgtcctgcg
     1681 cttcgcggct gatcttccat tgcaccgcga ccttctcggc cgtgatgccc atgccgtagg
     1741 cgatgccgat gttctcgtcg cgctcgaaga tggccgggtt gaccgagggc ttgccgccgc
     1801 ctatcggcac caggctcatc gactcggcgc cgccggcgat catcacgtcg gcctcaccga
     1861 cgcggatgcg gtcggccgcc atctggatcg cggtgatgcc ggacgcgcag aagcggttca
     1921 ccgtcacacc gccgatcggg ttcgggaacg cgagcgccac cgcgatgcgc gccatgttca
     1981 tgccctgctc gccttcgggg aacgagcagc cgatgatcgc gtcctcgatc gccgtcggat
     2041 cgagcgacgg cacctgcagc atcgcgctcc tgatcgcggc gaccagcagg tcgtcgggtc
     2101 gggtgttctt gaagtagccg cggcccgagc ggccgatcgg cgtgcgcgtc gccgcgacga
     2161 tgtaagcctc ttgcacttgc ttgttgctca tggtctggtc cttcgtgttc agttgcgcac
     2221 gggcttgccg gtctgcagca tgcccgtgat tcgttcttgc gtcttctcgt ggtcgagcag
     2281 cgcgcagaaa tgcttgcgct ccagcgccat caggtattcc tcgctgacca gcgaaccggc
     2341 atcgacatcg ccgccgcaga tcacatcggc gatcagcgcc gagaggtgga agtcgtgctt
     2401 gctgacgaag gcgccgtcgc gcatgttcgc gagctgcccc ttgatcgtcg cgatcgcgtt
     2461 gcggccggcg accgggaaca gcgtgcgcgc cggcgcgcgg tagccgctct cgaacatggc
     2521 cttggcctgc tgcgacgcga cgtacaacag ctcgtccttg ttcggcacga ccacgtcgct
     2581 ccacagcagg tagccgagtt tgcgcgactc gctcgcgctg gttccgacct tggccatcgc
     2641 cgcattcgtg aagccgtcgg acaggaactt gaagatgtcg gcagtcgcgc tgcccggcga
     2701 tgacgaacca gcggccatct cggccgcacg gcgcgcgatg taggtcaggc cgccgccgcc
     2761 cgggatcagc ccgacgccga cctcgaccag gcccatgtag ctctccatcg ccgcgacgcg
     2821 cttggccgca tagaccgcga tctcgcagcc gccgcccagc gcgacgccgc gcaccgccgc
     2881 gacgaccggc acggtcgcgt agcgcaggcg cagcatcgcg tcctgcaact tcttgatctc
     2941 gggcaggatg cccttgccgc cactcttcat gaacaccggc atcagctgtt cgagattcgc
     3001 accggccgag aacacgtcgt cgggcgacca gatcaccagg cccttgtacc cgcgctcggc
     3061 gagctcgacg gcctgggtca agccttcgac cacgccttgg ccgatcaggt gcagcttcga
     3121 ggtgatgctc gctatcaaga cctcgccgtc gagggtccag acgcgcatct cgtcgttctt
     3181 gaacacctcg gttcccgagg tcagcgccga cccggcgccc gaaccgaata ccgattcgcg
     3241 gaagtgctgg cgcgcgtaca cgggcagcgt gctcggcgcg acccagcgcg aatgcgcggc
     3301 gctccacgag ccctcggcac ggtgcacgcc gccgtgcgct gccggcgggc cgtcgaacac
     3361 ccaggccggc agcggcgcgc tgctcagtgc cttgccggcg gctatgtctt ccttgatcca
     3421 gttcgcgacc gtcgtccagc cggccgcctg ccacaactcg aacgggccct cgctgacgcc
     3481 gaaaccccag cgcatcgcga agtccacgtc gcgcgcgctc tcggcgatgt cggcgagatg
     3541 aaccgccgcg tagtggaagc tgtcgcgcag gatcgcccac aggaatgcgg cttgcgggtt
     3601 cttcgattca cgcaacagct tcagccgctc ggccggtgcc ttcttcagga tgcgcgcgac
     3661 gatctcgtcg gccttgccgc cgccggcgac atagctgccg ctggccgggt ccaggcgctg
     3721 gatgtccttg ccgacccgct tgtagaaacc ggcgccgctt ttctgcccca gcgcaccctg
     3781 ctcgatcagc ctggccagca ccggcggcgt cgcgtagctc gaatagaacg ggtcgtcttt
     3841 caggttgtcc tgcagcgtct tgatgacgtg cgacatcgtg tccaggccga ccacgtcggc
     3901 ggtgcggaag gtgccactgc tggcgcggcc gagcttcttg ccggtcaggt cgtccaccac
     3961 gtcgtagctc aggccgaagg tctcggcctc cttcatcgtc gccagcatgc cggcgatgcc
     4021 gacgcggttc gcgatgaagt tcggcgtgtc catcgcgcgc accacgctct tgcccagcgc
     4081 ggtggtcacg aaggcctcga gctggtcgag gatctccggt tgcgtggtcg gtgtgttgat
     4141 cagctcgacc agcgccatgt agcgcggcgg gttgaagaag tggatgccgc agaagcgcgg
     4201 cttgagcgcg tccggcagca cttccgacag cttggtgatc gacaggccgg aggtgttgct
     4261 cgcgacgatc gcgtgcttgg ccaccgccgg cgcgatgcgc ttgtacaggt cgagcttcca
     4321 gtccatgcgc tcggcgatgg cttcgatgat gaggtcgcag tccttcagca ggtcgaggtg
     4381 ctcctcgtag ttcgccggct ggatcaacgc ggcgtcttca gcgacgccca gcggtgcggg
     4441 cttgagcttg ccgaggcccg cgacggcctt cgtgacgatg ccgttcttcg ggccctcctt
     4501 ggcgggcagg tcgaacagga tcacgggcac cttgcagttg acgaggtggg cggcgatctg
     4561 cgcgcccatc acgccggcgc cgagcacggc caccttgcgc acggggaatc ggttcatttt
     4621 ttctccttgg ttgcgaacgc cggcgttcat tcgacgcggg tccaggcctg acggcggtaa
     4681 aacgggccga ggcccgctgc ggtgacgatt tggtgcaggt gtcggatcat ggtcgggtct
     4741 cccggttcgg attcgagcac gctcagaaca gcgcctcgtc catcgccatc agcggcgccg
     4801 cgccggcccg cgcggtgcgg atcagcgtcg cggtctcggg cagcagcttg gcgaagtaga
     4861 agcgcgcggt cgtcagcttg gccttgtaga acgggtcgcc gccgccgacc ttcgcgagcg
     4921 cgaccttggc catgcgggcc cagaaatacg cgaacaccag gtggccgcag acgcgcaggt
     4981 agtcgaccgc cgcggcgccc gcctcgtcgg ggttcttcat cgccttcatg ccgatctcgg
     5041 tcgtcagctt cgtgaccttg tcgcccaggt cggcgagcgg gttgacgaac tcctgcatgt
     5101 cctcgttcac gccttcgtcc tcgacgaacc gcgcgaccag cttgccgaac ttcttcagct
     5161 tcgcgccgtt gtcgccgagc accttgcgcc ccagcaggtc gagcgactgg atcgtgttgg
     5221 tgccctcgta gatcatgttg atgcgcgcgt cgcgcacgaa ctgctccatg cccgactcgg
     5281 cgatgtagcc gtggccgccg aacacctgca tgcagtgcga ggtcgagagc cacgcgttgt
     5341 cggtcaggaa ggccttgacg atgggcgtga ccagcgcgac caggtcggcg cactcggccc
     5401 gcaccgcggg gtcgtcgctg gcgagttcct tgtcgagctg cagcgtcgtc cacatcgcca
     5461 gcgcgcggcc gccttcggca taggcacgcg cggtcagcag catcttgcgc acgtcggggt
     5521 gcacgatgat cgggtcggcc ggcttgtcgg gcgccttcgg gccggacagc gaacgcatct
     5581 ggatgcgctc cttcgcgtag gcagccgcgt tctggtacgc gacctcggtc aggcccaggc
     5641 cctgcatgcc cacgccgagg cgtgccgcgt tcatcatcac gaacatcgcg gccaggccct
     5701 tgttcggctc gcccaccagc gtgccgaccg cgccgtcgag gttgatctgc gcggtggcgt
     5761 tgccgtggat gcccatcttg tgctcgaggc cgccgcagaa gatcgggttg cgcgcgccta
     5821 agcgtccgtc ggcatcgacg aggaacttcg gcacgacgaa cagcgagatg cccttcgagc
     5881 cggccggcgc atcgggcagc cgggcgagca cgaggtggat gatgttggcg gccaggtcgt
     5941 gctcgccggc cgagatgaag atcttgttgc cggtgagctt gtaggtgccg tcggtctgcg
     6001 gttccgcttt cgtgcgcagc atgcccagat cggtaccgca gtgcgcctcg gtcaggcaca
     6061 tcgtgccggt ccactcgccg ctcgtgagct tgggcaggta ggtcttcttc tgcgcgtccg
     6121 agccatgggc gcgcagcgcc tcgtaggcgc cgtgcgacag gcccgggtac atggtccagg
     6181 cctgattcgc cgagttcatc atctcgaaca tgcactggtt caccaggtgc ggcaggccct
     6241 ggccgccgta ggccgggtcg cagctcagcg ccggccagcc gccttcgacg tactgtgcgt
     6301 aggcctgctt gaagcccttc ggcgccgtga cctcgtgggt ggtcttgtcg agcacgcagc
     6361 cctgctcgtc gccgatgcgg ttcagcggcg cgagcacggt ggccgcgaac ttgccgcctt
     6421 cctcgacgac ggcgttgatg gtgtcgacat cgatgtcggc gtgcgccggc agtgccttca
     6481 gctcgtcgac gacgttgagc agctcgtgca tcacgaactg catgtcgcgc agcggcggcg
     6541 tgtattgggg catgggcaat ctcctgaagg cgaaatcaag tcgagaccgg gccgcgcctg
     6601 ccgcgcgcgg cgacgggcga tgcaggcagc gacggcgcgg cgaggtagtg cgacagcacg
     6661 ttctcgaagc cggtccgtgc acgcgcctcg gcgcccgagt ggcgcagaaa gcgcgcgtcg
     6721 tggtgcagcg ccaggatcag gccgtggatc tcgaacagca tctgctgcgg gtcggtatcg
     6781 gcgcgcagat ggccctcgtc gaccgcgatg cggatcgcgc gctcgagcgc gttctgccag
     6841 gtctgcacca tgctgacgag cgcgtcgcgc accgggccgg gccggtcgtc gaattcgacc
     6901 gcaccgctga tgtagatgca gccggcgtcg atctcgatcg agacgcgctt gaaccagcgg
     6961 tcgtagagcg cgcgcagccg cggcaggccg cgcggctcgc gcatcgccgg ctcgaagatc
     7021 tcgtccgaga acttcgcgtg gtactcgcgc accaccgaga tctgcagttc ctcgcgcgag
     7081 ccgaaatgcg cgaacacgcc ggacttgctc atctgcgtga cctcggcgag cgcgccgatc
     7141 gacaggcctt ccagtccgat ctgcgaggcc agcgcgagcg cggcgtcgag gatcgcggcg
     7201 cgcgtctgcc gccccttcat cagcgggcgc ggcgcggcgg tgcgggcggc gcgggtcgcc
     7261 tcgttcaggg tcgtcgtggg gtcggtcacg ggcttgcttg gtcaggtgcg cggcatgaaa
     7321 acgaacggtc gtgctatttt gcgcaggatc gggcggcccg ggccggccga gcgggcggat
     7381 ttcgcatgtt tttctaggcc ttgccgccta gaacagggcg atcactgggc tcgcccaagg
     7441 gtttaccctg agtccgacgc cgggcgccgc gatggctacc atcgtccgct cgccaacagg
     7501 cttggcgcgc cgagggcacg gatgaaaatt gcggtggtgg ggctgggggc cgtcggcggc
     7561 ctgatcgcag cgcggctcgc gatggccgag ttcgaggtct gcgcgctgac gcgcggcgcc
     7621 acgctcgccg ccgtgcgcgc gcacgggctg cggctcgtgg cgggtggcga agagaaggtg
     7681 gcgcgcatcg cggcgagcga cgacgcacgc acgctcggcc cgcaggacct ggtgatcgtc
     7741 gcgctcaagg gcccggccct ggcagccgcg gccgcgtcgc tgcagccgct gctcggttcg
     7801 cagaccatcg tgatgccggc gatgaacggc gtgccgtggt ggttcctcga cgctgcgccg
     7861 gccgatgcgg cactgggcga ccggcacctg gccagcatcg accccgacgg ccggatcggt
     7921 gccgcgctgc cgctcgcgcg cgtgctcggc tgcgtcgtgc acctgacctg ttcgagcccc
     7981 gagccggggt tggtccggca cggcttcggc gaccggctga tcgtcggcga gccgggcggg
     8041 ggcccgtcgg cgcgcgtcga catggtccgc gcggccttgg gccatgccgg cttccaggcc
     8101 gaggccagca ccgacatccg ccaggccatc tggtacaagc tgtggggcaa catgacgatg
     8161 aacccggtgt cggcactgac cggcgccgag agccaccaca tcatcgacga cccgctggtg
     8221 ctggccttca tgctgggcac gatggaagag gcgcggcgca tcggcgcccg catcggctgc
     8281 gtgatcgaac agtcgggcga ggagcggatg gcggtggcac gcgagctcgg tgcgttcaag
     8341 acctcgatgc tgcaggactg cctcgccggg cggccgctcg agctcgatgc actggtcggc
     8401 gcggtgcacg agatcggcac gcgcctcggc gtggcgatgc ccaacatcgg cgcgctgctc
     8461 ggtctgacgc ggctgatggc gcgcgagcgc ggcctgtatc cgccggctgc atgacagccg
     8521 gatgacgcgt cgcgccgccg cagcgctcga gcgcgccggg gttgagggaa tgcagcgtct
     8581 gcattacgct cgaagcctca tggaagtact gcaactcgat gtatcgggcc ggccgcaggc
     8641 ctggatctcg gcgaaggaag cggcgatcct ctacgcgagc gacggcgtcg cctggacgct
     8701 cggcgacacc ttttacgtgt tgcgcggcgg ctatcagcgc accgggctgc agtcgcgcat
     8761 cgaggtgcac ccgatcatcg cggtgcgcgg ctcggtgccg agccgggcct ggcgccagac
     8821 gccggcgctg tc
//
LOCUS       DATMJB010000037         3037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_467356, whole genome shotgun sequence.
ACCESSION   DATMJB010000037 DATMJB010000000
VERSION     DATMJB010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467356"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1084)
                     /locus_tag="VNU48_00860"
     CDS             complement(<1..1084)
                     /locus_tag="VNU48_00860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HWI09860.1"
                     /translation="MHPNALLELATELIHQVLQLKHPADGVVSDFFRQHRTLGSRERH
                     TLAETTYSVLRERLLYQHLAQSGKGEMERRLAVLAWQGNEGFLRAALSETEQQWLAQV
                     GTVDRAALPDKLRHNLPEWLAERLQAAVGDEFWPLVEAMNQGAGLDLRVNTFKAKRDE
                     VQAAFKAENIEALPTPYSPLGLRIDGKPALHKLDVFMRGDVEVQDEGSQLLALLTGAK
                     RSEMVADFCAGAGGKTLALGAEMRNTGRLYAFDTSGHRLAALKPRLARSGLSNVYPIQ
                     IAHERDERIKRLAGKLDRVLVDAPCSGLGTLRRNPDLKWRQTPQAIEELRAKQAAILA
                     SAARLLKPGGRLVYATCSLLRAENEDI"
     gene            1106..1246
                     /locus_tag="VNU48_00865"
     CDS             1106..1246
                     /locus_tag="VNU48_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HWI09861.1"
                     /translation="MSDALPDSDLPALPASGWWVRPHAMFFGPVTIAGRVQPRFSRVG
                     AA"
     gene            1243..1896
                     /locus_tag="VNU48_00870"
     CDS             1243..1896
                     /locus_tag="VNU48_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014900052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HWI09862.1"
                     /translation="MSDFFAHVRSRPRLLIAVLVGLLAALLVPGTDHAVTRSLLGWNV
                     GVWLYLVLAVVLMLRADHEHLRRAAIAQSESAATVLTLVIVAAVASIVAIVIELAAAK
                     QGSQHGWSHVLFALVTVAGSWMLLPTLFAMNYATLYYRGGHGCGLRFPEPDEPGFRPD
                     YADFLYFSFTIAVASQTADVSITSRSMRRLVLLQSVLSFAFNTTILALTINIAAGLF"
     gene            complement(1908..2534)
                     /gene="purN"
                     /locus_tag="VNU48_00875"
     CDS             complement(1908..2534)
                     /gene="purN"
                     /locus_tag="VNU48_00875"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HWI09863.1"
                     /translation="MKRIAILISGRGSNMQAIVRACAAEGWDARVVAVIANRADAQGL
                     AFARQHGIATEVVDHKGFASREAFDAELARTLDGFAPDVVALAGFMRILGDAFVERFA
                     GRLINVHPSLLPAFTGLHTHARAIAAGCKLAGATVHFVTPALDHGPIIAQAVVPVLPD
                     DTPALLAARVLEREHVLYPRAVRWLVQGLLRVDDGVVTHTGGEAQLLR"
     gene            2577..>3037
                     /locus_tag="VNU48_00880"
     CDS             2577..>3037
                     /locus_tag="VNU48_00880"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345774.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWI09864.1"
                     /translation="MKPRVLSGMRPTGALHLGHYHGALKNWVRLQHEHDCYFFVADWH
                     ALTTHYEDHADLERNVYDMVVDWLAAGIDPERATLFIQSRLPEHAELFTLLAMGTPLS
                     WLERVPSYKDQIRSLSGRNLATYGFLGYPLLQAADILIYKAAYVPVGEDQAP"
BASE COUNT          416 a         1106 c         1040 g          475 t
ORIGIN      
        1 cgatgtcctc gttttcggcc cgcagcaggc tgcaggtcgc gtagacgagc cggccgccgg
       61 gcttcagcag gcgcgccgca ctcgcgagga tcgccgcctg cttcgcccgc agctcctcga
      121 tcgcctgcgg cgtctggcgc cacttcaggt cggggttgcg gcgcagcgtg ccgaggccgg
      181 agcaaggcgc gtcgaccagc acccgatcga gcttgccggc caggcgcttg atgcgctcgt
      241 cgcgctcgtg cgcgatctga atcgggtaga cgttcgacag cccgctgcgc gcgagccgcg
      301 gcttcagcgc cgcgagccgg tgaccggagg tgtcgaacgc gtagagccgg ccggtgttgc
      361 gcatctccgc gccgagcgcg agcgtcttgc cgccggcgcc ggcgcagaag tcggcgacca
      421 tctcgctgcg tttggcgccg gtcagcagcg cgagcagctg gctgccttcg tcctgcacct
      481 cgacatcgcc gcgcatgaac acgtcgagct tgtgcagcgc gggcttgccg tcgatgcgca
      541 agcccagcgg cgaatacggc gtcggcaacg cctcgatgtt ctcggccttg aacgcggcct
      601 gcacctcgtc gcgcttggcc ttgaaggtgt tgacacgcag gtcgagcccg gccccctggt
      661 tcatcgcctc gaccagcggc cagaactcgt cgcccaccgc cgcctgcagc cgctcggcca
      721 gccactccgg caggttgtgg cgcagcttgt cgggcagcgc ggcgcgatcg accgtgccga
      781 cctgcgccag ccactgctgc tcggtctcgc tcagcgccgc gcgcaggaag ccctcgttgc
      841 cctgccaggc gagcacggcc aggcgccgct ccatctcgcc cttgcccgac tgcgcgaggt
      901 gctggtacaa cagccgctcg cgcagcaccg aataggtcgt ctcggccagc gtgtggcgct
      961 cgcggctgcc cagcgtgcgg tgctggcgga agaagtcgga caccacgccg tcggccgggt
     1021 gcttcagctg caggacctga tggatcagtt cggtggcgag ttcgagcaag gcgttcgggt
     1081 gcatcggcgg attatccttg gggccatgtc cgacgccctg cccgacagcg acctccccgc
     1141 cctgcccgcc agcggctggt gggtgcggcc gcacgcgatg ttcttcgggc cggtcacgat
     1201 cgccggccgg gtccagccgc gcttcagccg ggtcggcgcc gcgtgagcga cttcttcgcc
     1261 cacgtcaggt cgcgcccgcg cctgttgatc gcggtgctgg tcggcctgct ggcggcgctg
     1321 ctggtgcccg gaaccgacca cgcggtgacg cgctcgctgc tcggctggaa cgtcggcgtc
     1381 tggttgtacc tggtgctggc ggtcgtgctg atgctgcggg ccgatcacga acacctgcgc
     1441 cgcgccgcga tcgcgcagtc cgagagcgcg gcgaccgtat tgacgctggt catcgtcgcg
     1501 gccgtcgcca gcatcgtcgc gatcgtcatc gagctcgcgg ccgccaagca gggcagccag
     1561 cacggctggt cgcacgtgct gttcgcgctg gtgaccgtgg ccggctcgtg gatgctgctg
     1621 ccgacgctgt tcgcgatgaa ctacgcgacg ctgtactacc gcggcgggca cggctgcggt
     1681 ctgcgattcc ccgagcccga cgaaccgggc ttccggcccg actacgccga cttcctgtac
     1741 ttctcgttca cgatcgcggt cgcctcgcag accgccgacg tctcgatcac gagccgctcg
     1801 atgcgccgcc tggtgctgct gcagtcggtg ctgtcgttcg ccttcaacac gacgatcctg
     1861 gcgctgacga tcaacatcgc cgccggcctg ttctgaaccg gcgctcgtca gcgcagcagc
     1921 tgcgcctcgc cgccggtgtg cgtgacgacg ccatcgtcga cgcgcagcag cccctgcacc
     1981 agccagcgca ccgcgcgcgg gtacagcaca tgctcgcgct ccagcacgcg cgcggccagc
     2041 agcgccggcg tgtcgtcggg cagcaccggc acgaccgcct gcgcgatgat cgggccgtgg
     2101 tccagcgccg gcgtgacgaa gtgcaccgtc gcgccggcca gcttgcagcc ggccgcgatc
     2161 gcccgggcat gagtgtgcag cccggtgaac gccggcagca gcgacgggtg gacgttgatc
     2221 agccggcccg cgaagcgttc gacgaaggcg tcgccgagga tgcgcatgaa gccggccagc
     2281 gcgaccacgt cgggcgcgaa gccgtcgagc gtgcgcgcca gttctgcgtc gaaggcctcg
     2341 cgcgacgcga agcccttgtg gtcgaccacc tccgtcgcga tgccgtgctg gcgcgcgaac
     2401 gccaggccct gcgcatcggc gcggttggcg atcaccgcga ccacgcgcgc atcccagccc
     2461 tcggcggcgc aggcgcgcac gatggcctgc atgttggagc cgcggccgga gatgaggatg
     2521 gcgattcgct tcacgggcgc gcagtgtaca gagcacccgg cctacactcg catcgcatga
     2581 agccacgggt tctctccggc atgcgcccga ccggcgcgct gcacctcggc cactaccacg
     2641 gcgcgttgaa gaactgggtg cggctgcagc acgagcacga ctgctatttc ttcgtcgccg
     2701 actggcacgc gctgaccacc cactacgagg accacgccga cctcgagcgc aacgtctacg
     2761 acatggtggt cgactggctc gcggccggca tcgaccccga gcgtgcaacg ctgttcatcc
     2821 agagtcgtct gcccgaacac gccgagctgt tcaccctgct cgcgatgggc acgccgctgt
     2881 cgtggctgga gcgcgtgccg agctacaagg accagatcag gtcgctcagc ggccgcaacc
     2941 tcgcgaccta cggcttcctg ggctacccgc tgctgcaggc ggccgacatc ctgatctaca
     3001 aggccgccta cgtgccggtc ggcgaagacc aggcgcc
//
LOCUS       DATMJB010000038        15686 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_585544, whole genome shotgun sequence.
ACCESSION   DATMJB010000038 DATMJB010000000
VERSION     DATMJB010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 15686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15686
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585544"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="VNU48_00885"
     CDS             complement(<1..264)
                     /locus_tag="VNU48_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HWI09865.1"
                     /translation="MTSLTLFSPAGVLVKAAPLRLAAKRLESYGFDVRIDESALARHQ
                     RFAGDDEVRLAALHRVAQQAPGIALASRGGYGLTRLLDRIDWQL"
     gene            complement(273..842)
                     /gene="tadA"
                     /locus_tag="VNU48_00890"
     CDS             complement(273..842)
                     /gene="tadA"
                     /locus_tag="VNU48_00890"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HWI09866.1"
                     /translation="MSAADEQAMRIALDQAQNAWLVGEVPVGAVIMRAGQVIATGYNR
                     PITEHDPTAHAEIVALRHAAQLLANYRLPECELYVTLEPCAMCAMALLHARVKRVVFG
                     ARDPKTGAAGSVVNLFEQPQLNHHTALEGGVLADECAVVLREFFAERREQYRQRRAVT
                     GTGPDTVPGEIPEIPAGEATEIGTVPPSP"
     gene            complement(847..1176)
                     /locus_tag="VNU48_00895"
     CDS             complement(847..1176)
                     /locus_tag="VNU48_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09867.1"
                     /translation="MRMIFGVLSLLVVLAIMGTVAKKQLQALSTSGVSTRISQMASGA
                     QGSAATGGDRDGATFAIPGGMPGGVAADTGGLTVPQQAQNIQQQFKQSTERALQQGVD
                     RNARSSP"
     gene            complement(1318..2565)
                     /locus_tag="VNU48_00900"
     CDS             complement(1318..2565)
                     /locus_tag="VNU48_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09868.1"
                     /translation="MNRSGTLPLFDDDEPAKSLSLVEVLAGHKPATKAQQTFHKLVLQ
                     IERKREQLAQWQAATLRHDARVAAEMLPLQARLQQRQRDMAVLIDTLLSQPTPGQRRG
                     KVQRAKLRQLLLNLLTGLLEDGDDPTLEALYDKHADVPLAQERQDEMDLAQAMLEDVF
                     GMEVDADHGASTAEELFAHAQRKMHEGARERAHAQQQQREERAAKRGKAGAAKADAAQ
                     ARREQAAAEVGQSVREVYRKLASALHPDREAEPQARQRKTLLMQRVNQAYDANDLLTL
                     LGLQLEIEQIDAEHLASVPAQRLAHFNQVLREQLAELDTELARCMAPFRAGRGYGPTP
                     TPAAIDQEFNVELAQLRAALREIEADLVAFRDPAQLRAALTHYRLERDDDDALDPADL
                     DELLGALDLPTPAPRPSRRRHKS"
     gene            complement(2743..3198)
                     /locus_tag="VNU48_00905"
     CDS             complement(2743..3198)
                     /locus_tag="VNU48_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09869.1"
                     /translation="MVADAFCSTAVFAPCMRRRKVSPLLGLRRKSLIAFAQALQRSLS
                     LCHDLTSELFLVVLDQRTGNRKATTSNARNTVSLTIRTIGLKPIVANSPGAVRRGKRR
                     KGYHEPWQQRRLGGAGALMSLPPATPARSWSIWMGWATWRSVQKLLLYV"
     gene            3231..3362
                     /locus_tag="VNU48_00910"
     CDS             3231..3362
                     /locus_tag="VNU48_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09870.1"
                     /translation="MRQSVEQLRRGSQILERLIETDDLLVVGAENSPETGRVEIFDD"
     gene            3427..3891
                     /locus_tag="VNU48_00915"
     CDS             3427..3891
                     /locus_tag="VNU48_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWI09871.1"
                     /translation="MTISTLSLATLSDWVGKELGTSKWVTVDQDRINQFAQCTGDEQW
                     IHVDVKRAEKESPFGGPVAHGYLTLALLVDLGMDIGVVPKDAKAAFNYGLDKVRFLAP
                     VKSGSRVRARISLVELTEQGGGRKLLKLNNTVEIEGEPKPALIAETLAVLVG"
     gene            4133..5761
                     /locus_tag="VNU48_00920"
     CDS             4133..5761
                     /locus_tag="VNU48_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI09872.1"
                     /translation="MNPLIGIRASDFGGAAQTLFGAAVKQPVNAALHWGAYAKELAKV
                     ASGMSDASHDPKDKRFADPAWQANPLLKRLLQVHAATGKELDRYIDATALNARDKARA
                     HLVASIFVDTIAPSNTLLNPTALKRALETGGVSLLKGVKNLAHDLRYNKGLPASVDKS
                     AFAIGRNLALTPGNVVFRNEVLELIQYRPTADKVHARPLVITPPQINKFYALDLSPEK
                     SLFKFVAAEGLQLFAISWCNPTKAQRDWNMSTYVQALDRAVDVARQITGSPDVNLWGA
                     CSGGMTAAAYLGWLAATGQHKVASVIAPVCVLDAARTMDTTMGLMASEKGFKAIKAAV
                     KRKGVVEGAELARVFAWLRPNDLIWNYWVNNYLLGNNPPPFDILYWNADTTRLPAAFH
                     ADLLDMLENNPFVNAGEMKVLGEPIDMSQVNVEAYVVAGITDHITPWKACYETARVYG
                     PESEFILTNAGHLQSLLSAPGTPKSFFFSAKAGGPEPDAWAQSAARHEGSWWPHWMAW
                     MRQRSGALIPAPKKLGSRKHPAGIAAPGDYVMVP"
     gene            5770..6654
                     /gene="phaZ"
                     /locus_tag="VNU48_00925"
     CDS             5770..6654
                     /gene="phaZ"
                     /locus_tag="VNU48_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006112 - energy reserve metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) depolymerase"
                     /protein_id="HWI09873.1"
                     /translation="MASGPQRSAADRLTEVRIDPGSGLHIGTVQIGRQFLRVGIRPLR
                     PGVVPSGPPLLLFNGIGANMEVTAPLTSALDGVETLIFDLPGAGKSPPPLFPYRLFSM
                     ARLTRKLLDVLGYGEVDVLGVSWGGGLAQQFAIQYPQRVRRLVLAATAMGGATMLPGH
                     PRILLKMINPRRYLDKGYMKQVAPELYGGDMRRNPDAIKLFTDHARGGDARGYRYQLL
                     AMLGWTSLPWLWRIRHPTLVLAGSDDPLVPLINARLHAWLLRDARLHVVDDGHLFLLT
                     RAQETAAAISGFLAEPAT"
     gene            6699..8165
                     /locus_tag="VNU48_00930"
     CDS             6699..8165
                     /locus_tag="VNU48_00930"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HWI09874.1"
                     /translation="MSSAVLADSSDAIRAAPQPAQGRRRGFLSVQQECDGIDLRVQGR
                     LPEWLVGTYVLNGPAQWELPKRSYAHWFDGLAMLHGFRIQPGRVHYRNRFLRSQDWRE
                     SHANGRPQLGGYDTAPAGGLLSRIVHMFNPRRSDNGCVVAMHQDGQWFALTESDRVMR
                     FDGQTLDTQGEVAWTEGGKLPLLAAHPCIDAQGRWWNVGVSLGRECEYVLFSCDVRGH
                     RAVKARIPVKRAGYLHAFAMTATHTVIWECAWRAHPLRFLFAGESYARHFDWMPEGGS
                     RLHTVRLSDGAVRSWDAPPLVLFHASQAFDAGGDVVVDLCLNDEPVVEELHIARLRAG
                     MPVTSMLARHARFVLSPGASTAREEALPGRFELPQVNARVATRGAARYVWGATTADST
                     PGEFFNRTLRLDLQTGEMHQAGRDDAFALEPLFVPNPNGIAEDDGVLLVHTLADDDPG
                     SRIRVLDAATLDERAAIELPHVIPFGFHGAWLAVRPIR"
     gene            8375..8899
                     /locus_tag="VNU48_00935"
     CDS             8375..8899
                     /locus_tag="VNU48_00935"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWI09875.1"
                     /translation="MKVFRSLIALWGVAVLSGAATSAHALDKLPVLSLEAARKMVAGC
                     EAKAKEMKWKMNVAVVDNGANLIIFERMDGAFLGGSDLARRKAETSAKFPFTTRFVEE
                     ISYGRGGKDGLDRKGGPIPGFALAPGLVAFPGGMPIIVGGTHVGGIGVSGGLPDEDEL
                     CAKAGLESAKDMLQ"
     gene            9006..9869
                     /locus_tag="VNU48_00940"
     CDS             9006..9869
                     /locus_tag="VNU48_00940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI09876.1"
                     /translation="MLAAMAQAAEQPLSGRVALVTGANSGIGLVTARELARQGAHVFI
                     ACRSAERAAPALADIRAASGSDRVELLSIDLGDFDSVHRCAQAFLARGLPLHLLINNA
                     GLAGAQGLTKSGFELAFGVNHVGHFLLTQLLLPCIRRSAPARIVTVASKAHYRVRSIP
                     WDELRKPTQAKTGMPEYRVSKLANVLFSAELARRLAGSGVSTYALHPGVVASDVWRSV
                     PWPLRSLIKLLMLSTEEGAATTLHCATSAEVGRESGLYYDKCRPQEPSALAQDPALAA
                     ELWKRSEAWTG"
     gene            complement(10009..12840)
                     /locus_tag="VNU48_00945"
     CDS             complement(10009..12840)
                     /locus_tag="VNU48_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWI09877.1"
                     /translation="MFKTLKGRTALAAALLAGIGIALVTSLQSNLSERSVIASTVLQH
                     EAYTARVAGEIDGRLRLARASLSEFAANVPGSELRDSGTLHYYLTSRVGIQQAFESIA
                     VYDIDGKMIASRPSIPTTSIAAEPWFAASLRKAVVGPPTRSRLSQDTVVELTYRVYDE
                     AGKLRGVAVGTLPINHEQLFSAAARDTSRGHFVLVTRDARVVLHPDATKVGQSIDALG
                     PAAAIIRGGLQAAAHSVAGPDSQGVRSLYAFHPISSADWTLVGVVSNEEAYASLDRLS
                     RQMLLAGGLLALLLIPAMWVLVARMLLPLDELRREMRRLKDGGDVAEPGHKRHGITQE
                     LQQVAEEFAGMAAARHNAEASLQQEKERAEVTLHSIGDAVLATDRAGLVTAMNTAAER
                     LTGWALHEALGRPFADVVVVCDEASAEPLPDLAAAAMRDGEIVSLPQAVLRARSGELL
                     PIDNSAAPIRSASGVVDGAVVVLRNVAVERAATQELNWRANHDAMTGLMNRIAYENAL
                     KQLVEAMQDGDEHSVIMIDLDKFKIVNDTCGHAGGDELLKQLARLFLNLTRKTDLVVR
                     LGGDEFAVLMYQCPSDNAIQLAEKLLRAVAEWRFEWEGQTFRVGASIGVVSVDKSFAD
                     ATAVQKAADMACYMAKRTGRNRICIHSNDNHAVEEVRLQMQQVARIQDAIDAGRLRLY
                     GQSIRAVDERLQLGQHFEVLLRMLDDTGKLVAPGDFLPAAERYGLMDQIDRWVIEHTI
                     ETCAGRFGPDRWGELDTASVNLSALSLRDAGIAGFIIDALRRHGMPARCLCIEITETA
                     MLESLSMVRELLQGLRATGVRIALDDFGVGMTSLSHLRELPVDVLKIDGSFVKGIHRD
                     ALNSDIVDAIQRIARRLDMQTVAECVEEASELEHLRGLGVHFVQGYLLARPAPLGMVI
                     GVAEPQPQRLRVDRQPALASQAIESMSVA"
     gene            13067..14038
                     /locus_tag="VNU48_00950"
     CDS             13067..14038
                     /locus_tag="VNU48_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HWI09878.1"
                     /translation="MSVIRNSFVAGLVLCCANAWADTYPSRTITLVVPFSANGPTDTI
                     AHSVGGAMSKHLKQAVVIENVGGAGGTVGAAQVAQADPDGYTMLIYHVGMATAPALYP
                     TLPFDPLRDFEFIGEVTDVPMTLVARNDFPAHTFKDAVAYVKANRDKVKLAHAGSGSA
                     SHLCGLLLMAAIEVDLKTVAYKGTGPAMDDLLAGKVDLMCDQTTNTTTQIRAGKVKAL
                     GITTTRRIASLPGVATLQEGGLKDFQVAVWHALYVPKGTPPAAVKRLTEALQAALQDS
                     GVKARFNDLGTDPVALEKATPQYVRQHLSTEIKRWEPIIKRSGQYTD"
     gene            complement(14417..14974)
                     /locus_tag="VNU48_00955"
     CDS             complement(14417..14974)
                     /locus_tag="VNU48_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09879.1"
                     /translation="MLAALADKVFTPERLKEMLRELKARLKKAQSGQDDQTKVLQREL
                     VELEQATSRLYEAVEKGFLPMDDTLTARAQKLKSRREALMIDLAGVRRAKEMPASMLT
                     QANVDAFGTALRARLQAGEGGFPKRYLRQFVSDIRYDGKRLTMTGRKDALLIAALEKK
                     MGTAGVPTSSLSWLPDLGSNQGPTD"
     gene            complement(14929..>15686)
                     /locus_tag="VNU48_00960"
     CDS             complement(14929..>15686)
                     /locus_tag="VNU48_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWI09880.1"
                     /translation="VMVRQVFALFDEYQSKENAKHVLRAMKENARQGYFNGSRTSFGF
                     KKEELDLVAAKGKKKRIVIDETEAQTVRRVFDLYLHGLNGNEMGSKQIAAHFNERGPS
                     LRGARWTRTRVHNMLSDTAYMGEYVFNKKESRTLQAKSEDEWVRVPIEAIVPPEVFLA
                     VKAKRHQRSPQVTPARVVNSPTLLTGLLRCANCGAGMTLATGKGGRYRYYKCNTRIGQ
                     SIGSCSTPAMRPLPRWIARCLRHWLTRCSRRSV"
BASE COUNT         2550 a         5303 c         5207 g         2626 t
ORIGIN      
        1 cagctgccag tcgatccggt cgagcaaccg cgtcagcccg tagccgccac gactggcgag
       61 cgcgataccc ggcgcctgct gcgcgacgcg gtgcagcgcg gccagccgca cctcgtcgtc
      121 gcccgcgaag cgctgatgcc gcgccagcgc cgattcgtcg atgcggacat cgaagccgta
      181 ggactcgagc cgcttcgcgg ccagccgcag cggcgccgcc ttgacgagca cgccggccgg
      241 cgagaacagg gtcagcgagg tcatggatgc gctcacggtg acggcgggac ggtcccgatc
      301 tcggtggcct cgccggccgg gatctcgggg atctcgccgg gcaccgtgtc gggcccggtg
      361 ccagtcactg cgcggcgctg ccggtactgc tcgcgccgct cggcgaagaa ctcgcgcagc
      421 accaccgcgc actcgtcggc gagcacgccg ccttcgagcg cggtgtggtg gttcagctgc
      481 ggctgctcga acaggttgac gaccgaaccg gccgcgccgg tcttcgggtc gcgagccccg
      541 aacacgacgc gcttgaccct tgcgtgcagc agcgccatcg cgcacatcgc gcagggctcg
      601 agcgtcacat acagctcgca ctcgggcagg cggtagttcg ctaggagttg cgcggcatgg
      661 cgcagcgcga cgatctcggc atgtgcggtc gggtcgtgct cggtgatcgg gcggttgtag
      721 ccggtggcga tcacctgccc ggcgcgcatg atcaccgcgc cgaccggcac ctccccgacc
      781 agccacgcgt tttgcgcctg gtcgagcgcg atgcgcatcg cctgctcgtc ggcagcgctc
      841 atcaactcac ggcgacgagc gcgcgttgcg atcgacaccc tgctgcagcg cgcgttcggt
      901 gctctgcttg aactgctgct ggatgttctg cgcctgctgt ggcaccgtca ggccgccggt
      961 gtcggcggcc acgccgcccg gcatgccgcc gggaatcgcg aaggtcgcgc cgtcgcggtc
     1021 gccgccggtc gctgcgctgc cctgtgcgcc gctcgccatc tggctgatgc gcgtggacac
     1081 gccgctggtg ctcagcgcct gcagctgctt cttcgcgacc gtgcccatga tcgccagcac
     1141 caccagcagc gacaacactc cgaagatcat tctcatggtc cgactcctgt gggcgcgcgc
     1201 cccgcgatgg ctcgtgccgt attctgcctc gcgggcccag caatcagcgt ggaccggctg
     1261 cctactttgc aacggccatg gcccggccgc gaggtgccgc gacctcgaac ggcgccctca
     1321 cgacttgtgc cgccgcctcg aagggcgcgg cgccggcgtc ggcagatcca gcgcgccgag
     1381 cagttcgtcc aggtcggccg gatcgagcgc gtcgtcatca tcacgctcga ggcggtagtg
     1441 cgtcaacgcg gcgcgcagct gcgccgggtc gcgaaacgcg accaggtcgg cctcgatttc
     1501 gcgcagcgcg gctcgcaact gggcgagttc gacgttgaac tcctgatcga tcgccgccgg
     1561 ggtcggcgtc gggccgtaac ccctgccggc gcgaaacggc gccatgcagc gcgccagttc
     1621 ggtgtcgagt tcggcgagtt gctcgcgcag gacctggttg aaatgcgcca ggcgctgcgc
     1681 cggcacgctg gcgaggtgct cggcatcgat ctgctcgatc tcgagctgca aacccagcag
     1741 cgtcagcagg tcgttggcgt cgtaggcctg gttgacgcgc tgcatcagca aggtcttgcg
     1801 ttgccgtgcc tggggttcgg cctcgcggtc ggggtgcagc gcgctggcga gcttgcgata
     1861 gacctcgcgc accgactggc cgacctcggc ggcggcctgc tcgcgtctgg cctgcgccgc
     1921 atcggccttc gcggcgccgg ccttgccacg cttggcggca cgctcctcgc gctgctgctg
     1981 ctgcgcgtgc gcgcgctccc gggcgccctc gtgcatcttg cgctgcgcat gcgcaaacaa
     2041 ctcctcggcg gtcgatgcgc cgtgatcggc gtcgacctcc atgccgaaca cgtcttccag
     2101 catggcctgg gccagatcca tttcatcctg gcgctcctgc gccagcggca catcggcgtg
     2161 cttgtcgtag agcgcttcga gcgtcggatc gtcgccgtct tcgagcaggc cggtgagcag
     2221 gttcagcagc aactggcgca gcttggcgcg ctgcaccttg ccgcggcgct ggccgggggt
     2281 cggctggctc aacaaggtgt cgatcagcac ggccatgtcg cgctggcgct gctgcagcct
     2341 ggcctgcagc ggcagcatct cggcggccac gcgcgcgtcg tggcgcagcg tcgcggcctg
     2401 ccattgcgcc agttgctcgc gtttgcgctc gatctggagc accagcttgt gaaaagtctg
     2461 ctgcgccttg gtcgcgggct tgtggccggc gagaacctcg acgagcgaca acgacttggc
     2521 cggttcgtcg tcgtcgaaca gcggcagggt gcctgatcga ttcatcgcta ctcccactcg
     2581 attgtcaatc gatggttgcc cggggccacc gaagcgggcg agcaacgata ccgtcgtaaa
     2641 cacgtcattc cgacggcgct cttcgtccga agcgagaaca ctttatccag attcatgcac
     2701 gaatcgtaca ggcctcgcga tcggcctggc gggacacata cctcagacgt acagaagcag
     2761 tttctgtact gaacgccacg tcgcccaccc catccaaatg ctccacgacc tggcaggcgt
     2821 tgcaggcggc aacgacatga gcgctccggc accgccgagc cgccgctgct gccatggttc
     2881 gtggtagcct ttgcgccgct tgccgcggcg aacagcgccg ggggaatttg ccactatggg
     2941 ctttaggccg atcgtgcgga tggtcagcga gaccgtgttc ctggcattgc tggtggtcgc
     3001 tttcctgttc ccggtgcgct gatccaacac gacgaggaag agttcggagg tgaggtcatg
     3061 acagagggac agcgagcgct gcagcgcctg cgcgaaggca atcaacgatt tgcgtcgaag
     3121 gcccaacagc ggtgaaacct tcctgcgccg catgcacggt gcgaagaccg ctgtggagca
     3181 gaaggcgtcg gccaccattc tgggctgctc cgattcgcgt gtgcccggtc gtgcgccagt
     3241 cggtcgagca gctgcgccgc gggtcgcaga tcctcgagcg cctgatcgaa actgacgacc
     3301 tgctcgtagt cggcgcggag aattcgcccg agaccggccg ggtcgagatc tttgatgact
     3361 agcgcagtct gcagtcccat ggaccctttc acgtactggc ctattgacct acaaggagga
     3421 aggcacatga ccatctctac gctctctctc gccacgctct cagactgggt cggcaaggaa
     3481 ctcggcactt ccaagtgggt gaccgtagac caggaccgca tcaatcagtt tgcccaatgc
     3541 acgggggacg agcaatggat ccatgtcgat gtcaaacgcg ccgagaagga gagccccttc
     3601 ggcgggccgg tggcgcatgg ctacctgacg ctggcgctgc tggtcgacct gggcatggac
     3661 atcggcgtcg tgccgaagga cgccaaggcg gctttcaatt acgggctgga caaggtgcgc
     3721 ttcctggcgc cagtcaagtc gggctcgcgc gtacgcgccc gcatcagcct ggtcgaactc
     3781 accgaacagg gcggcgggcg caagctgctc aagctgaaca acacggtgga aatcgagggt
     3841 gaacccaaac ccgcactcat cgctgagaca ctggcggtgc tggtgggctg aaaacggatc
     3901 ggcgaaatac aaactacaaa caaacaaaca ggagacgaca acaatggtca gcaaatcaaa
     3961 gcttccagcc aaaaccgcct cgcgccgcat ggcagccaag acagcgaagc ccgagccagg
     4021 gccgcgcgca aaggttgcgg caaccggtgc tgcgccccgc cttcgcagcg tcgatgccgc
     4081 cgaacccgtg tcggaacgcc tgggcgagag cgcggtacgc aacacactcg cggtgaatcc
     4141 gctgatcggc attcgcgcga gcgacttcgg cggcgcggcg cagacgctgt tcggcgcagc
     4201 agtcaagcag ccggtcaatg cggcgctcca ctggggcgcc tatgccaagg aactcgccaa
     4261 ggtcgcgagc ggaatgtccg acgcttcgca cgacccgaag gacaagcgct tcgccgaccc
     4321 ggcctggcag gccaacccgc tgctcaagcg cctgctgcag gtgcatgccg ccaccggcaa
     4381 ggaactcgac cgctacatcg acgccaccgc gctgaatgca cgcgacaagg cgcgtgccca
     4441 tctggtggct tcgatcttcg tcgacacgat cgcgccgagc aacaccctgc tcaacccgac
     4501 ggcgttgaaa cgcgcgctgg aaaccggcgg tgtcagcctg ctcaagggcg tgaagaacct
     4561 ggcccacgac ctgcgctaca acaaggggct gccggcgtcg gtcgacaagt cggccttcgc
     4621 catcggccgc aacctggccc tgacgccagg caacgtggtc ttccgcaacg aggtgctgga
     4681 actgatccag taccggccca cggcagacaa ggtccacgcg cggccactgg tcatcacgcc
     4741 accgcagatc aacaagttct atgcgctgga cctgtcgccg gagaagagcc tgttcaagtt
     4801 tgtcgcggcc gagggcctgc agttgtttgc catcagctgg tgcaacccga ccaaggcaca
     4861 gcgcgactgg aacatgtcca cttacgtgca ggcgctggac cgggctgtcg acgtggcgcg
     4921 gcagatcacc ggcagccccg acgtcaacct gtggggcgcc tgttccggcg gcatgacggc
     4981 ggcggcctac ctgggctggc tggccgcaac cgggcagcac aaggtggcca gcgtgatcgc
     5041 gccggtgtgc gtgctcgacg cggcgcgcac catggacacc acgatggggc tgatggccag
     5101 cgagaaggga ttcaaggcga tcaaggccgc cgtcaagcgc aagggtgtcg tcgaaggcgc
     5161 cgagttggca cgcgtcttcg cctggctacg gccgaacgac ctgatctgga actactgggt
     5221 caacaactac ctgctcggca acaatccgcc gcccttcgac atcctctact ggaacgcgga
     5281 caccacgcgg ttgccggcgg ccttccacgc cgacctgctc gacatgctcg agaacaaccc
     5341 cttcgtcaat gccggcgaga tgaaagtgct gggcgagccc atcgatatga gtcaggtgaa
     5401 tgtcgaggcc tacgtcgtcg ccggcatcac cgaccacatc acgccctgga aggcgtgtta
     5461 tgaaacggcc cgcgtttacg gacccgagag cgagttcatc ctgaccaatg ccgggcacct
     5521 gcagagcctg ctcagcgcgc ccggcacccc aaagtccttt ttcttctccg ccaaggccgg
     5581 cgggccggag ccggacgcct gggcccaaag cgcagcgcgc cacgaaggca gctggtggcc
     5641 gcactggatg gcctggatgc ggcagcgttc gggcgcgctg attcctgcgc cgaagaagtt
     5701 gggcagtcgc aagcaccctg cgggcattgc cgcgccgggc gactacgtca tggtgccatg
     5761 agctgccgca tggcgagcgg cccgcaacgc tcggccgcgg accggctgac ggaggtgcgc
     5821 atcgaccccg gctcgggtct gcacatcggt acggtgcaga tcggcagaca gtttctgcgc
     5881 gtcggcatcc ggcccctgcg gccgggtgtc gtgccgtccg gcccgccgtt gctgctgttc
     5941 aacggcattg gcgcgaacat ggaagtgaca gcgccgttga cttcggcgct cgatggggtc
     6001 gagacgctga tcttcgacct gccgggtgcc ggcaagtcac cgccgccgct cttcccgtac
     6061 cgcctgtttt cgatggcccg cctgacgcgc aagcttctcg atgtgctcgg ctacggcgag
     6121 gtcgacgtgt tgggcgtgtc gtggggcggc gggctggcgc agcagttcgc gatccagtac
     6181 ccgcagcgcg tgcgccgtct ggtgctcgcc gccacggcga tgggcggggc gaccatgctg
     6241 cccgggcacc cgcgcatact gctgaagatg atcaacccgc gccgctatct ggacaagggc
     6301 tacatgaagc aggtggcgcc cgagctgtac ggcggcgaca tgcgccgcaa cccggacgcc
     6361 atcaagctgt tcaccgacca tgcgcgtggc ggcgacgcca gaggctaccg ataccagctg
     6421 ctggccatgc tcggctggac cagcctgccc tggttgtggc gcatccgcca tccgacgctg
     6481 gtgctggccg gcagcgacga tccgctggtg ccgctgatca atgcccggct gcatgcctgg
     6541 ctgctgcgcg acgcgcggct gcatgtggtc gacgacgggc atttgttcct gttgacccgc
     6601 gcgcaggaga cggctgcggc catctccggg tttctggctg agcccgcaac gtgagcgcac
     6661 aacatgcgtg tgctgactga tcggcgcgcg gagatgccat gagcagcgcc gttctcgccg
     6721 attcatccga tgccatcaga gccgcgccac agcctgcaca gggacgccgc cgcggtttcc
     6781 tgtcggtgca gcaggagtgt gatggcattg atctgcgggt tcagggccga ctgcccgagt
     6841 ggctggtcgg cacctatgtc ctgaacggcc cggcgcagtg ggagttgccc aagcggtcct
     6901 acgcgcactg gttcgacggc ctggcgatgc tgcacggctt tcgcatccag cccggccgtg
     6961 ttcactaccg caaccgcttc ctgcgctcgc aggactggcg cgaatcccac gccaacggcc
     7021 gcccccaact cggcggttac gacaccgcgc cggcgggcgg cctgctgtcg cgcatcgtgc
     7081 acatgttcaa cccgcgccgc agcgacaacg gctgcgtggt ggcgatgcac caggacggcc
     7141 aatggttcgc gttgaccgag tcggaccgcg tgatgcgctt cgacggccag acgctggaca
     7201 cgcagggcga agtggcctgg accgaaggcg gcaagctgcc gctgctggcc gcgcacccct
     7261 gcatcgacgc gcagggccgc tggtggaacg tgggtgtctc gctgggacgc gagtgcgagt
     7321 acgttctgtt cagctgcgac gtccggggac accgcgcggt gaaggcgcgc attccggtca
     7381 agcgcgcggg ctacctgcac gccttcgcga tgaccgccac gcacacggtg atctgggagt
     7441 gtgcgtggcg cgcacatccc ttgcgattcc tgttcgcggg cgagtcgtat gcgcggcact
     7501 tcgactggat gcccgagggc ggttcgcgcc tgcacacggt gcgcctgtcg gacggtgcgg
     7561 tgcgttcgtg ggacgcgccg ccgctggtgt tgtttcatgc cagccaggcc ttcgacgccg
     7621 gcggcgacgt ggtggtggac ctgtgcctga acgacgaacc ggtggtcgaa gaactgcaca
     7681 tcgcccggct gcgcgcgggc atgccggtca cgtccatgct ggcgcgccac gcccgcttcg
     7741 tgctgtcgcc gggcgcatcc accgctcgtg aagaggcctt gccggggcgg ttcgaactgc
     7801 cgcaggtgaa cgcccgcgtc gcgacccggg gcgctgcacg ttatgtgtgg ggcgcgacca
     7861 cggccgacag cacacccggc gagtttttca accgcacgct caggctcgac ctccagaccg
     7921 gcgagatgca ccaggccggg cgcgacgacg cgttcgccct ggagccgctg ttcgttccca
     7981 acccgaatgg catcgcggaa gacgacggcg tgctgctggt ccacaccctg gccgacgacg
     8041 accccggctc acgcatccgc gtgctcgatg ccgcaacgct ggacgagcgc gccgcaatcg
     8101 aactgccgca cgtgattccg ttcggctttc acggcgcgtg gctggcggtc aggccgatac
     8161 gctgagggat gtgccggggc tggtatcgaa tcgccttggc aaggggacca cgcgagacgg
     8221 cctcttgcac atgcgtcgag atcgtcaaag gccgacttga ttcaagtcgc gtcattcgat
     8281 tgtttctcca tatacttcgt tcgccgcgac gcatgacagg agtacgttca ccgatcctgc
     8341 cgacatggga acaacatcca ttcgaggaga caagatgaag gtttttcgtt ctctcatcgc
     8401 gctgtggggc gtggccgtgc tgagcggcgc cgcgacaagc gcccatgcac tcgacaagct
     8461 gccagtgctg tccctcgagg cggcgcgcaa gatggtggcc ggatgcgagg ccaaagccaa
     8521 ggagatgaag tggaagatga acgtcgcggt tgtcgacaac ggcgccaacc tgatcatctt
     8581 cgagcggatg gatggcgcat tcctgggcgg cagtgacttg gcgcggcgca aggcagaaac
     8641 gtcggccaag ttcccgttca cgacgcgctt cgtcgaagag atctcgtacg gcaggggcgg
     8701 caaggacggc ctggaccgca agggcggacc gattcccggg ttcgcactcg cgcccggcct
     8761 cgtggccttc ccgggcggca tgccgatcat cgttggcggc acccacgtgg gcggcatcgg
     8821 agtcagtggc ggcctcccgg acgaagacga actctgcgcc aaggccgggt tggaaagcgc
     8881 gaaagatatg ttgcagtaga ggcttcgttt gggtcgtccg ctgcccgaga ggtgtcgtgc
     8941 gcgagcgcgg ccggccgggt cgccgcttct gaacccgatg tgaacgacgt tggcggactc
     9001 gcgctatgct cgccgccatg gcacaagccg cagaacaacc tctcagcgga cgggtggcgc
     9061 tggtcaccgg tgcgaactcc gggatcgggc tggtcaccgc acgcgagctc gcgcggcaag
     9121 gcgcgcacgt gttcatcgcc tgccgctccg ccgaacgcgc ggcaccggcg ctggccgaca
     9181 ttcgcgcagc gagcgggagc gaccgcgtcg agctgctttc gatcgacctc ggcgacttcg
     9241 attcggtgca ccgatgcgca caggcctttc tcgcgcgcgg gctgccgctg cacctgctga
     9301 tcaacaacgc cggcctggcc ggcgcgcagg gcttgacgaa gtcgggcttc gagctcgcct
     9361 tcggcgtcaa ccacgtcggc cacttcctgc tgacccagct gctgctgcca tgcatccggc
     9421 gatcggcacc ggcacgcatc gtgacggtgg cgagcaaggc ccactaccgc gtccgcagca
     9481 tcccttggga cgagcttcgc aagccgaccc aggcaaagac cgggatgccc gaataccgcg
     9541 tctccaagct ggcgaacgtg ctgttcagtg ccgagctcgc gcgccggctc gcgggcagcg
     9601 gcgtctcgac ctatgcgctg caccccggcg ttgtggcgtc cgacgtctgg cgctcggtgc
     9661 cctggccgct gcgctcgctg atcaagctgc tgatgctctc caccgaagaa ggcgcggcca
     9721 ccacgctgca ctgcgccacc tcggccgagg tgggccgtga gagcgggctg tactacgaca
     9781 agtgccggcc ccaggaaccg agcgcgctgg cgcaggaccc ggcactcgct gccgagttgt
     9841 ggaagcgcag cgaagcctgg accggttgaa cggtggcatt gggccggctc gagaggtccc
     9901 aggagcgggc gcgcgtcgca gcgcccgggc atgtcgatcg gcctggcgat gcgggtggtg
     9961 cgccagcagc gccgcacgag cgctgcgaca agcttcgcgg cttgcagatc aggcgaccga
    10021 catcgactcg atggcttgcg atgccagggc cggctgccga tcgacgcgca ggcgctgcgg
    10081 ctgcggctcg gccacgccga tgaccatgcc cagcggcgcg gggcgcgcca gcaggtagcc
    10141 ctggacgaag tgcacgccga ggccgcgcag gtgctccagc tcgctggctt cctccacgca
    10201 ttcggccacg gtctgcatgt cgagccgccg cgcgatgcgc tggatcgcgt cgacgatgtc
    10261 gctgttgagc gcgtcgcggt ggatgccttt gacgaagctg ccgtcgatct tgagcacgtc
    10321 gaccggcagt tcgcgcagat gtgacaagga cgtcatgccg accccgaaat cgtccagcgc
    10381 gatgcgcacg ccggtggcgc gcaggccttg caaaagttcg cgcaccatgc tcaggctttc
    10441 gagcatggcc gtctcggtga tttcaatgca caggcagcgc gccggcatgc cgtggcgtcg
    10501 cagtgcgtcg atgatgaagc cggcaatgcc cgcatcgcgc aggctcaggg ccgacaggtt
    10561 caccgacgcg gtgtcgagtt cgccccaacg atcgggcccg aagcgcccgg cgcaggtctc
    10621 gatggtgtgc tcgatcaccc agcggtcgat ctggtccatc agcccatagc gttcggcggc
    10681 gggcagaaag tcgcccggcg cgaccagctt gccggtgtcg tcgagcatgc gcagcagcac
    10741 ttcgaagtgt tgccccagtt gcaggcgctc gtcgacggcg cgaatggact ggccgtacag
    10801 gcgcagccgg cccgcgtcga tcgcatcctg aatccgggcg acctgctgca tttgcaggcg
    10861 cacctcttcg accgcatggt tgtcgttgga gtgaatgcag atgcggttgc gcccggtgcg
    10921 cttggccatg tagcaggcca tgtcggcggc tttctggact gccgtggcgt cggcaaaact
    10981 cttgtcgacc gagacgacac cgatgctggc gcccacccgg aaggtctggc cttcccactc
    11041 gaagcgccac tcggcaaccg cgcggagcaa tttctccgcg agctggatcg cgttgtcgct
    11101 cgggcactgg tacatcagca ccgcgaactc gtcaccgccc agtcgcacca ccagatcggt
    11161 cttgcgcgtc aggttcagga acaggcgtgc gagctgcttg agcaactcgt cgccgccagc
    11221 atggccgcag gtgtcgttga cgatcttgaa cttgtccagg tcgatcatga tcaccgagtg
    11281 ctcgtcgccg tcctgcatcg cctcgaccag ttgcttgagc gcgttctcgt aggcgatgcg
    11341 gttcatcagc ccggtcatgg cgtcgtggtt ggcgcgccag ttgagttcct gggtcgcggc
    11401 gcgttccacc gccacgttgc gcagcacaac gacggcgccg tcgaccacac cgctggccga
    11461 gcgtatcggc gccgccgagt tgtcgatcgg cagcagctcg ccactgcggg cgcgcagcac
    11521 ggcctgcggc agcgagacga tttcgccatc gcgcatggcc gcagccgcca ggtcgggcaa
    11581 gggctcggcg ctggcttcgt cgcacaccac caccacgtcg gcgaagggcc gccccagcgc
    11641 ctcgtgcaag gcccagccgg tcaggcgctc ggcggccgtg ttcatggccg tcacgaggcc
    11701 ggcccggtcg gtggccagca cggcgtcgcc gatcgaatgc agcgtcactt cggcgcgctc
    11761 cttctcctgc tgcagcgagg cttccgcgtt gtggcgcgcg gcggccatgc cggcgaactc
    11821 ttcggccact tgctgcagct cctgcgtgat gccgtggcgc ttgtggcccg gctcggcaac
    11881 gtccccaccg tctttcaagc ggcgcatttc gcgccgcagc tcgtcgagcg gaagcagcat
    11941 gcgcgcgacc agcacccaca tggccggaat cagcagcagc gccagcaggc cgccggccag
    12001 cagcatctgt cgcgacaggc ggtcgagcga cgcataggcc tcttcattgc tcaccacacc
    12061 gaccagcgtc caatcggccg aactgatggg gtggaaggca tacaggctgc gcacgccctg
    12121 gctgtcgggc ccggccaccg agtgcgcggc ggcctgcagc ccgccgcgga tgatcgcggc
    12181 ggccgggccg agcgcgtcga tcgactggcc gaccttcgtg gcatcggggt gcagcaccac
    12241 cctggcgtcg cgcgtcacca gcacgaagtg cccgcggctg gtgtcgcgcg cagccgcgct
    12301 gaacaactgt tcgtggttga tgggcaaggt gccgaccgcg acgccgcgca gcttgccggc
    12361 ttcgtcgtag acgcggtagg tcagctcgac caccgtgtcc tggctgagcc ggctgcgagt
    12421 cggcggcccg accacggcct tgcgcaggct ggcggcgaac cagggctcgg ccgcgatcga
    12481 ggtggtcggg atcgatggcc gcgacgcgat catcttgccg tcgatgtcgt agaccgcgat
    12541 gctctcgaag gcctgctgga tgccgacccg cgaggtcagg tagtagtgca gcgtgccgga
    12601 gtcgcgcagc tcgctgccgg gaacgttggc ggcgaactcg ctcagcgagg cccgcgccag
    12661 ccgcaagcgg ccgtcgatct caccggccac gcgggccgtg taggcctcgt gctgcagcac
    12721 cgtcgaggcg atgacgctgc gttcggacag gttcgactgc aggctcgtga ccagcgcaat
    12781 cccgatgccg gccagcagcg cggcggccag agcagttcga cctttcaggg tcttgaacac
    12841 gagtcagtgt ctccattccg acagcacaac gaggcgaacg acccaggggt ttgttgtgcc
    12901 agccccgatc cgcgcgccat ccctctggcc gtcgcggtca aaggggatat cggccgagct
    12961 gggcgtgtcc ttagcgcgtc acgcggggcg agagcggccg cctcagtcaa gttttgccgc
    13021 ggcgtgccga aatcgatggc gtcgccaacc ctgccctgga tccgccatgt ccgtcattcg
    13081 caactcgttc gtggccggcc tggtcttgtg ctgcgccaac gcgtgggccg acacctaccc
    13141 cagccggacc atcaccctgg tcgtgccgtt ttccgccaac ggccccaccg acacgattgc
    13201 acacagtgtg ggcggggcga tgagcaaaca cctgaagcag gcggtcgtca tcgaaaacgt
    13261 cggcggcgcc ggcggcacgg tgggtgccgc gcaggtcgcg caggccgacc cggacggcta
    13321 cacgatgttg atctaccacg tcggcatggc cacggcgccg gcgctgtacc cgaccctgcc
    13381 gttcgatccg ctgcgcgact tcgaattcat cggcgaggtc accgacgtgc cgatgacgct
    13441 cgtggcccga aacgattttc cggcccatac cttcaaggac gcggtcgcct acgtcaaagc
    13501 gaaccgcgac aaggtgaaac tggcgcatgc cggctcgggc tcggcctcac acctgtgcgg
    13561 cttgttgctg atggcggcga tcgaagtcga cctgaagacc gtggcctaca aaggcaccgg
    13621 cccggcgatg gacgatctgc ttgcgggcaa ggtcgacctg atgtgcgacc agaccaccaa
    13681 caccaccacg cagatccggg ccggcaaggt caaggcgctg ggcatcacca ccacgcgccg
    13741 gatcgcgtcg ctgccgggtg tcgcgacctt gcaggaaggc gggctgaagg acttccaggt
    13801 cgccgtgtgg cacgcgctgt acgtgcccaa gggcacgccg cccgccgcgg tcaaacgttt
    13861 gaccgaagcc ctgcaagcgg cgctgcagga ctcgggcgtg aaagcgcgct tcaacgacct
    13921 cggcaccgac ccggtggcgc tggaaaaagc aacaccgcag tacgttcgcc agcacctgag
    13981 cacggagatc aagcgctggg agccgatcat caaacgctcg ggtcagtaca ccgactgaag
    14041 ccgtcgccgc cgccctgccc gagaagtccc caaaacaacc cccctcactc ccactctatt
    14101 gtcaatggtc ggtcgcggct cagagtgaaa gcgcgctgcg tgccggccga gaacaggcac
    14161 gataccgaca gatttaccgt cgcgttcgcg tgtgcctccc ccggctgttt gctctcgatc
    14221 tccctcatcg acggttcgcg acagcgattg gagggcgaaa ttggcatgtc ccgtaacgca
    14281 atgacggtat cgactgctcg ctccagaggg cgaagacggt attcaagagc ggtttgacgg
    14341 tatcacgacc ttccgtcgtc gtgactcgag cggttcacgc cgatcggtgg acacggcgca
    14401 ggcatgatgc tggctgttaa tccgtaggtc cctggttcga gcccaggtcg gggagccaag
    14461 acaggctaga agtgggcacc cctgcggtgc ccattttctt ttccagcgca gcgatcagca
    14521 gcgcgtcctt cctgccggtc atcgtgagcc gcttgccgtc atagcgaatg tcactgacga
    14581 actgccgcag gtagcgcttt gggaagccgc cctctcccgc ctgcaaacgt gcacgcagcg
    14641 ccgtaccgaa ggcatcgacg ttcgcctggg tcagcatcga tgccggcatc tccttggctc
    14701 gacgcacgcc ggccaggtcg atcatcaatg cctcgcgccg cgacttgagc ttttgcgccc
    14761 gagcggtcag cgtgtcatcc atcggcagga accccttttc tacagcctca tagagtcggc
    14821 tggtcgcctg ttccaactcc accagctcgc gctgcaacac cttcgtctga tcgtcctggc
    14881 cggactgcgc cttcttgagc cgcgccttca gttctcgcag catctccttc aaacgctccg
    14941 gcgtgaacac cttgtcagcc agtgccgcaa gcaccgcgcg atccatcttg gcaacgggcg
    15001 catggccggc gtgctgcagg agccaatgct ctggccgatg cgcgtattgc acttgtagta
    15061 gcgatatcga ccgcccttgc ctgtcgccaa ggtcatgccc gcgccgcagt tcgcgcaccg
    15121 cagtaagccc gtcagcaagg tcggcgagtt gacgacccgt gccggcgtga cctgcggcga
    15181 acgctggtgc cgcttggcct tgaccgccag gaacacttcg ggcggcacga tggcctcgat
    15241 cggcacgcgt acccattcat cctccgactt cgcctgcaga gttcgagact ccttcttgtt
    15301 gaagacgtac tcgcccatgt aggcggtgtc cgagagcatg ttgtggacgc gggttcgcgt
    15361 ccacctcgca ccgcgcaggg acggcccgcg ctcgttgaag tgggccgcga tctgcttgga
    15421 gcccatttcg ttgccgttca ggccatgcag gtacagatcg aagacccggc gaacggtctg
    15481 cgcttcggtc tcgtcgatca cgatgcgctt cttcttgccc ttggccgcaa ccaggtcgag
    15541 ttcttccttc ttgaacccga acgaggttcg cgagccgttg aagtagccct ggcgcgcgtt
    15601 ctccttcatc gcccggagga catgcttcgc gttctccttg gactggtatt cgtcaaacag
    15661 cgcaaagacc tgccgcacca tcacgc
//
LOCUS       DATMJB010000039         2245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_354966, whole genome shotgun sequence.
ACCESSION   DATMJB010000039 DATMJB010000000
VERSION     DATMJB010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354966"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="VNU48_00965"
     CDS             <1..1313
                     /locus_tag="VNU48_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWI09881.1"
                     /translation="TIVNVAVPGMSRVFALGQERAQWLSAGFMAAMTLSMLTTPWLLQ
                     RFGYRHTYIGVVALLLTGAVIGGCSPWFELVLAMRVAEGLAAGVLQPIPAIVVMRAFE
                     PAEQGRAMGIFGFGVVLAPAIGPSVGGVLVEWWGWRSIFFVVVPFCLVALVLARRYLP
                     VSAPGGGAVDRDGARLDVVGLLLIAIAVLALLNGMVQLHGASGATGGALLACSATALI
                     GFVFHQKRGARPLMELRLFGHRNFALGGLVAFIYGMALFGSTYLVPVFMQIALRLPPS
                     QAGAALLPAGIVLAATIPLAGRLTDRLPFGRIVAFGLSLLAASFLLMLTVGPASSLWW
                     IVAWAVIGRIGLGFVLPSLNVGSMRGLPDEFISQGASTINFMRQLGGAVGISLVGIVL
                     EWRLQVHAGDSLRAFHETFLLVGLITGAAVLAALRMRPAPPRAA"
     gene            1361..>2245
                     /locus_tag="VNU48_00970"
     CDS             1361..>2245
                     /locus_tag="VNU48_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWI09882.1"
                     /translation="MNLGIESLPGSCDVLVVGAGPAGSAAATLLARGGLDVVLIDQHA
                     FPRDKVCGDGLIPDAHNALRKLGVLDAVMAQAQASGFVGCIGPRAGRIDVPGELAVLP
                     RKQLDDIVCRAAVAAGARMFAPLRFVAPLESGGTVVGAGLQQGQTTREMRARWVLLAT
                     GAVPQALLAAGMSQRHTPSGVALRGYVKNDAMVGRVTALEIVWHRALAPGYGWIFPCR
                     DGVFNIGVGIADSHTEQRNGKLTKGELNLRQVMDDFQRVYAPARELMQGGTLLGAMKG
                     APLRCSLEGARYTRPGLLV"
BASE COUNT          243 a          788 c          851 g          363 t
ORIGIN      
        1 cgaccatcgt caatgtcgcg gtgcccggca tgagccgtgt gttcgcgctc ggccaggagc
       61 gggcgcagtg gctctcggcc ggcttcatgg cggcgatgac gctgtcgatg ctgaccacgc
      121 cgtggctgct gcagcgcttc ggctaccgcc acacctacat cggcgtggtg gcgctgttgc
      181 tcaccggcgc cgtgatcggc ggctgcagcc cgtggttcga gctggtgctg gcgatgcgcg
      241 tcgccgaagg gctggcggcg ggcgtgctgc agccgatccc ggcgatcgtc gtgatgcgcg
      301 cgttcgagcc cgccgagcag ggccgcgcga tgggcatctt cggcttcggc gtggtgctgg
      361 cgccggcgat cggcccgagc gtcggcggcg tgctggtcga gtggtggggc tggcgctcga
      421 tcttcttcgt ggtcgtgccg ttctgccttg tcgcgctggt gctggcgcgg cgctacctgc
      481 cggtcagcgc gcccggcggc ggcgcggtcg accgcgacgg cgcgcggctg gacgtggtcg
      541 gcctgctgct gatcgcgatc gcggtgctgg cgctgctcaa cggcatggtg cagctgcatg
      601 gcgcatcggg cgcgaccggc ggggcgctgc tcgcgtgcag cgccacggcg ctgatcggct
      661 tcgtgttcca ccagaagcgc ggtgcgcgcc cgctgatgga gctgcgcctg ttcggccacc
      721 gcaacttcgc actcggcggc ctggtcgcgt tcatctacgg catggcgctg ttcggctcga
      781 cctacctggt gccggtgttc atgcagatcg cgctgcgcct gccgcccagc caggccggcg
      841 cggcgctgct gccggcgggc atcgtgctgg cggcgacgat cccgctcgcc gggcgcctga
      901 ccgaccggct gccgttcggc cgcatcgtcg cgttcggcct gagcctgctg gccgcgtcgt
      961 tcctgctgat gctgacggtc gggccggcga gctcgctgtg gtggatcgtc gcgtgggcgg
     1021 tgatcgggcg catcgggctg ggcttcgtgc tgccgtcgct gaacgtcggc agcatgcgcg
     1081 gcctgccgga cgaattcatc tcgcagggcg cgagcacgat caacttcatg cgccagctcg
     1141 gcggtgcggt gggcatcagc ctggtcggca tcgtgctcga atggcgcctg caggtgcacg
     1201 ccggcgattc gctgcgcgcg ttccacgaga ccttcctgct ggtcggcctg atcaccggcg
     1261 ccgcggtgct cgcggcgctg cgcatgcggc cggcgccgcc gcgggctgcc tagaatcggc
     1321 gcggcatcct ggcatcgccc ttgcgcccgt gcgcaccctc atgaacctcg gcatcgaatc
     1381 cctccccggc agctgcgacg tgctcgtcgt cggcgccggc ccggccggca gcgccgcggc
     1441 gacgctgctc gcacgcggcg ggctggacgt ggtgctgatc gaccagcacg ccttcccgcg
     1501 cgacaaggtc tgcggcgacg gcctgatccc cgacgcgcac aacgcgttgc gcaagctcgg
     1561 cgtgctcgat gcggtgatgg cgcaggcgca ggccagcggc ttcgtcggct gcatcgggcc
     1621 gcgcgccggg cgcatcgatg tgcccggcga actcgcggtg ctgccgcgca agcagctcga
     1681 cgacatcgtc tgccgcgccg cggttgcggc cggcgcgcgc atgttcgcgc cgctgcgctt
     1741 cgtcgcgccg ctcgagtccg gcggcacggt cgtcggcgcg gggctgcaac aggggcagac
     1801 cacgcgcgag atgcgcgcgc gctgggtgct gctggccacc ggcgcggtgc cgcaggcgct
     1861 gctcgcggcc ggcatgtcgc agcgccacac gcccagcggc gtggcactgc gcggctatgt
     1921 gaagaacgac gcgatggtcg ggcgcgtcac cgcgctcgag atcgtctggc accgcgcgct
     1981 cgcgcccggc tacggctgga tcttcccgtg ccgcgacggc gtgttcaaca tcggcgtggg
     2041 catcgccgac agccacaccg agcagcggaa cggcaagctg acgaagggcg aactgaacct
     2101 gcgccaggtg atggacgact tccagcgcgt ctacgcaccg gcgcgcgagc tgatgcaggg
     2161 cggcacgctg ctcggcgcga tgaagggcgc gccgctgcgc tgctcgctgg aaggcgcacg
     2221 ctacacgcgg cccgggctgc tcgtg
//
LOCUS       DATMJB010000040         5512 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_351001, whole genome shotgun sequence.
ACCESSION   DATMJB010000040 DATMJB010000000
VERSION     DATMJB010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5512
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351001"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1199
                     /locus_tag="VNU48_00975"
     CDS             <1..1199
                     /locus_tag="VNU48_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWI09883.1"
                     /translation="NDSLGHQTGDALLKVVAQRLRESIREADLVSRFGGDEFVVLLNG
                     VTGSGEALQIAQRLRASLRAPYLIDTLELNVSCSIGIAMFPDDAADMDELMRHADTAM
                     YQAKASGRDSVYFFTRDLNDRAQQKLQIELLLRNAVARDELRLVFQPQVGAVGGALVA
                     VEALLRWNTPELGEVAPAVFIPLAEESRLIVPIGEWVIDQACRQLAAWRAHGVEVGVM
                     SVNLSAVQLLDPGLLPALRGSLERHRIPAELLELELTESTLMDSTDERLQQLHALRAL
                     GVQLSIDDFGTGYSSLSYLSRLPMGKLKIDRSLVKDMLTEPKDRAITEAIIALAHRLK
                     MTVVAEGVETAPVQTALADARCDGIQGYHTGRPMAPEALERWLAARTTPVGRNTARAA
                     APALLR"
     gene            complement(1243..2826)
                     /locus_tag="VNU48_00980"
     CDS             complement(1243..2826)
                     /locus_tag="VNU48_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI09884.1"
                     /translation="MKRASSAVLAAAAALALSLVGAGAHAQTLRWSSQGDLQTMDPDS
                     RNEQLTNSLNGQVYETLTGRDKELGIVPMLAVSWQQTGPLQWRVKLRANVKFHDGTPF
                     SADDVVFSAKRAAADGSDIKAYAAAMGEPKKIDDLTVEFNLKEVDPIFLQHANYIFIM
                     SKAWCEKNKVTKPQDFNAGEQSYAALNANGTGPYMLVSRQPDVKTVYKRNPNWWGKFE
                     GNVQDVIYTPIKSNSTRVAALISGELDFVLDPPPADTPRLRNTPNVKIIDGPENRIVF
                     IGMDQGRDELLYSSVKGKNPFKDQRVRQALYQAVDEESIKTKLMRGQAAPTGATMVSP
                     LGTYNDPALEKRLPFDLAAAKKLMTAAGYPDGFEVTLDCPNDRYVNDEEICKTLASMW
                     ARLGLKVRVNAMPRSVYFAKLGKLDTSMYMLGWGGSTTDAETTLTPIYRTRNGAIGQF
                     NYGQVNNPKMDQLAAQSSHEPDPVKREALIKAVIKEHNEQVHHIPLHRQFIPWATRAN
                     VEVVHRADNWLEWAWIKVK"
     gene            complement(3000..3659)
                     /locus_tag="VNU48_00985"
     CDS             complement(3000..3659)
                     /locus_tag="VNU48_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWI09885.1"
                     /translation="MKNPFRSIVALLALLATLGLSLSAAAQDAKAGEKKAAMCIGCHG
                     IPDYKATFPEVYRVPKIAGQNAKYIISALGQYKKGDRKHPTMRGIAASLSDQDIADLA
                     AFYATEAPSEPAPVALTGAVPGDVAELLKKGNCASCHGANFTTPIDPSYPKLAGQHGD
                     YLYIALREYQTDKNALVGRNNAIMMGMARPFTPAEAKILAAYFASLPTELKTVPQSRF
                     R"
     gene            3781..4623
                     /locus_tag="VNU48_00990"
     CDS             3781..4623
                     /locus_tag="VNU48_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWI09886.1"
                     /translation="MKFQGSENYVATPDLMLAVNAASTLKRPLLVKGEPGTGKTMLAE
                     EVALALNMPLLQWHIKSTTKAQQGLYEYDAVSRLRDSQLGDAKVKDIHNYIVKGVLWQ
                     AFTAERPVVLLIDEIDKADIEFPNDLLREIDRMEFYVYETHELVKAVHRPLVFITSNN
                     EKELPDAFLRRCFFHYIKFPDAETMKAIVGVHFPNLKKELLSAALKNFYDVRNLPGLK
                     KKPSTSELLDWLKLLLAEDIPLEVLQSKDDKVAVPPLVGALLKNEQDVSLFEKLVFMQ
                     RNNR"
     gene            4620..5210
                     /locus_tag="VNU48_00995"
     CDS             4620..5210
                     /locus_tag="VNU48_00995"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWI09887.1"
                     /translation="MSWLAPVELQGAHCALRPLSADHHDGLVEATRDGELWKLWYTAI
                     PGPDGMRAEIARRLDLQARGSMLPFTSFDAQGRIAGMTTYMNVDALHQRVEIGSTWTA
                     LSLQRGPFNTECKLLLLGHAFEELDCIAVEFRTHFFNQQSRRAIERLGAKLDGILRNH
                     QRASNGSLRDTCVYSVVQSEWPAVKAHLRWQLDKPR"
     gene            5318..>5512
                     /locus_tag="VNU48_01000"
     CDS             5318..>5512
                     /locus_tag="VNU48_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09888.1"
                     /translation="MLINFFFTLRAAKLKVSVKEYLMLLEAIQAGVIDNTVDEFYYLA
                     RSSLVKDEANFDKFDRAFAAY"
BASE COUNT          873 a         1834 c         1865 g          940 t
ORIGIN      
        1 tcaacgactc gctcggccac cagacgggcg acgcgctgct caaggtagta gcgcagcgcc
       61 tgcgcgaatc gatccgcgag gccgacctcg tcagccgctt cggtggcgac gagttcgtcg
      121 tgctgctgaa cggcgtgacc ggcagcggcg aggccttgca gatcgcgcag cgcctgcggg
      181 cctcgctgcg cgcgccgtac ctgatcgaca cgctcgaatt gaacgtgtcg tgcagcatcg
      241 gcatcgcgat gttccccgac gacgccgccg acatggacga gctgatgcgc catgccgaca
      301 ccgcgatgta ccaggcaaag gcgagcggcc gcgacagcgt ctacttcttc acccgcgacc
      361 tgaacgaccg ggcgcagcag aagctgcaga tcgagctgct gctgcgcaac gcggtcgccc
      421 gcgacgagct gcgcctggtg ttccagccgc aggtgggcgc ggtcggcggc gcgctcgtcg
      481 ccgtcgaggc gctgctgcgc tggaacacgc cggagctcgg cgaggtcgcg ccggccgtct
      541 tcatcccgct ggccgaagag tcgcgcctga tcgtgccgat cggcgaatgg gtcatcgacc
      601 aggcgtgccg ccagctcgcg gcctggcgcg cgcacggcgt cgaggtcggc gtgatgtcgg
      661 tcaacctgtc ggcggtgcag ctgctcgatc cggggctgct gccggcgctg cgcggctcgc
      721 tcgagcggca tcgcattccg gccgaactgc tcgaactcga gctgaccgag tcgacgctga
      781 tggacagcac cgacgagcgg ctgcagcagt tgcacgcgct gagggcgctc ggcgtgcaac
      841 tctcgatcga cgacttcggc accggctact ccagcctgag ctacctgagt cgcctgccga
      901 tgggcaagct gaagatcgac cgctcgctcg tcaaggacat gctgaccgag ccgaaggacc
      961 gcgcgatcac cgaggccatc atcgcgctgg cgcaccggct gaagatgacg gtcgtcgccg
     1021 agggcgtcga gaccgccccg gtgcagacgg cgctggccga tgcgcggtgc gacggcatcc
     1081 agggctacca cacgggccgg ccgatggcac cggaagcgct cgaacgctgg ctggcggcgc
     1141 gcacgacgcc cgtgggacgg aacactgcgc gggccgccgc gccggcgctg ctgcgctgat
     1201 cggggcccgc gtcgcaggcg tcggatcggc gccgggccgg gctcacttga ccttgatcca
     1261 ggcccactcc agccagttgt cggcgcggtg cacgacctcg acgttcgcgc gcgtcgccca
     1321 cgggatgaac tggcggtgca gcgggatgtg gtggacctgc tcgttgtgct ccttgatcac
     1381 ggccttgatc agcgcctcgc gcttgaccgg gtcgggctcg tggctcgact gcgccgcgag
     1441 ctggtccatc ttcgggttgt tgacctggcc gtaattgaac tggccgatcg cgccgtttcg
     1501 agtccggtag atcggcgtga gcgtcgtctc ggcatcggtc gtcgaaccac cccagcccag
     1561 catgtacatc gaggtgtcga gcttgcccag cttggcgaag tagaccgagc gcggcatcgc
     1621 gttgacgcgc accttgaggc ccaagcgtgc ccacatcgag gccagcgtct tgcagatctc
     1681 ctcgtcgttg acgtagcggt cgttcgggca gtcgagcgtg acctcgaagc catcggggta
     1741 gccggcggcg gtcatcagct tcttcgcggc ggccaggtcg aagggcaggc gcttctcgag
     1801 cgccgggtcg ttgtaggtgc ccagcggcga caccatggtc gcgccggtgg gcgcggcctg
     1861 gccgcgcatc agcttggtct tgatgctctc ctcgtcgacc gcctggtaca gcgcctggcg
     1921 cacgcgctgg tccttgaacg ggttcttgcc cttcacgctg ctgtagagca gctcgtcgcg
     1981 gccctggtcc atgccgatga agacgatgcg gttctcgggg ccgtcgatga tcttcacgtt
     2041 cggcgtgttg cgcagccgcg gggtgtcggc gggcggcgga tcgagcacga agtcgagttc
     2101 gcccgagatc agcgcggcca cgcgcgtcga gttgctcttg atcggcgtgt agatcacgtc
     2161 ctgcacgttg ccctcgaact tgccccacca gttcgggttg cgcttgtaga cggtcttcac
     2221 gtcgggctgg cgcgagacca gcatgtacgg gccggtgccg ttggcgttga gcgcggcata
     2281 gctctgctcg ccggcgttga agtcctgcgg cttggtgacc ttgttcttct cgcaccacgc
     2341 cttgctcatg atgaagatgt agttggcgtg ctgcaggaag atcgggtcga cctccttcag
     2401 gttgaactcg accgtcaggt cgtcgatctt cttcggctcg cccatcgcgg ccgcgtaggc
     2461 cttgatgtcc gagccgtcgg cggcggcgcg cttggccgag aacaccacgt cgtcggcgct
     2521 gaacggcgtg ccgtcgtgga acttgacgtt ggcgcgcagc ttcacgcgcc actgcagcgg
     2581 gccggtctgc tgccacgaca ccgcgagcat cggcacgatg ccgagttcct tgtcgcgccc
     2641 ggtcagggtc tcgtagacct ggccgttgag cgagttggtc agctgctcgt tgcgcgaatc
     2701 cgggtccatg gtctgcaggt cgccctgcga ggaccagcgc agggtctgcg cgtgcgcgcc
     2761 ggcgccgacc aggctgagcg ccagcgcggc cgcggcggcc agcaccgctg aggaagcacg
     2821 tttcattctg tcctgctccg gcaaatacga aaccggcagt ttaggcccgg cggctgccgc
     2881 acgtcgggca gggaagacct gcccgcgtcc gccaagggat cgagcaagtt ccaggccggc
     2941 gcggcgcgca gaaaaaagcc gctgacgcgc agcggctcgg gtttcgtgcg ctgtgcggct
     3001 cagcgaaagc gcgactgcgg caccgtcttc agctcggtcg gcagcgacgc gaagtacgcg
     3061 gccaggatct tcgcctcggc cggcgtgaac gggcgcgcca tgcccatcat gatcgcgttg
     3121 ttgcgcccga ccagcgcgtt cttgtcggtc tggtactcgc gcagcgcgat gtagaggtag
     3181 tcgccgtgct ggccggcgag cttcgggtag ctcgggtcga tcggggtcgt gaagttggcg
     3241 ccgtggcacg acgcgcaatt gcccttcttc agcagctcgg cgacgtcgcc cggcaccgcc
     3301 ccggtgagcg cgacgggcgc cggctcggac ggcgcctcgg tggcgtagaa cgccgcgagg
     3361 tcggcgatgt cctggtcgct cagcgacgcg gcgatgccgc gcatcgtcgg gtgcttgcgg
     3421 tcgcccttct tgtactggcc cagcgccgag atgatgtact tcgcgttctg cccggcgatc
     3481 ttgggcacgc ggtagacctc ggggaaggtc gccttgtagt cggggatgcc gtggcagccg
     3541 atgcacatcg ccgccttctt ctcgccggcc ttcgcgtcct gcgccgcggc gctcagcgac
     3601 aggcccagcg tggccagcaa ggccagcagc gcgacgatcg aacggaaggg atttttcatg
     3661 attccgggga gtggtgtttt cggcgcgttt tgcagttcag cgggcgatta tagggggccc
     3721 cttatactga cccgacaacc ccgaaccaca cctgggcggc cacgcatccg cccctccatc
     3781 atgaagttcc aaggctccga aaactatgtc gccacaccgg acctgatgct ggcggtgaac
     3841 gccgcatcga cgctgaagcg gccgttgctc gtgaagggcg aaccgggcac cggcaagacg
     3901 atgctggccg aggaggtcgc actcgcgctg aacatgccgc tgctgcaatg gcacatcaag
     3961 tccaccacca aggcgcagca aggcctgtac gaatacgacg cggtgagccg cctgcgcgac
     4021 agccagctcg gcgacgccaa ggtcaaggac atccacaact acatcgtcaa gggcgtgctg
     4081 tggcaggcct tcaccgccga gcggccggtg gtgctgctga tcgacgagat cgacaaggcc
     4141 gacatcgagt tcccgaacga cctgctgcgc gagatcgacc gcatggagtt ctatgtgtac
     4201 gagacgcacg agctggtgaa ggccgtgcac cggccgctcg tgttcatcac ctcgaacaac
     4261 gagaaggagt tgcccgacgc gtttctgcgc cgctgcttct tccactacat caagttcccc
     4321 gatgccgaaa cgatgaaggc aatcgtcggc gtgcacttcc cgaacctgaa gaaggagctg
     4381 ctgtcggcgg cgctgaagaa cttctacgac gtgcgcaacc tgccggggct gaagaagaag
     4441 ccgtcgacca gcgagctgct cgactggctc aagctgctgc tggccgagga cattccgctc
     4501 gaggtgctgc agagcaagga cgacaaggtc gccgtgccgc cgctggtggg tgcgctgctg
     4561 aagaacgagc aggacgtctc gctgttcgag aagctcgtgt tcatgcagcg caacaacagg
     4621 tgagctggct cgcgcccgtt gagttgcagg gcgcgcactg tgcgctgcgg ccgctgtctg
     4681 ccgatcatca cgacggcctg gtcgaggcca cccgcgacgg cgagctctgg aagctctggt
     4741 acaccgcgat ccccggcccc gacggcatgc gtgccgagat cgcgcgccgc ctcgacctgc
     4801 aagcccgcgg ctcgatgctg ccgttcacca gcttcgacgc gcagggccgc atcgccggca
     4861 tgacgaccta catgaacgtc gacgcgctgc accagcgcgt cgagatcggc tcgacctgga
     4921 ccgcgctgag cctgcagcgc ggcccgttca acaccgagtg caagctgctg ctgctcggcc
     4981 atgccttcga ggaactcgac tgcatcgcgg tcgagttccg cacccacttc ttcaaccagc
     5041 agagccggcg cgcgatcgag cgcctgggcg ccaagctcga cggcatcctg cgcaaccacc
     5101 agcgcgcgag caacggcagc ctgcgcgaca cctgcgtcta cagcgtcgtg caaagcgaat
     5161 ggccggcggt gaaggcgcac ctgcgctggc agctcgacaa gccgcgttga ccatcgggcg
     5221 atcgggctgg acggcatccc ggggtgaatg tcattccact cgcgccgagt atgcccgtgc
     5281 cgtcacaata gccgcatcgc cgccgatggt gcccgccatg ctgatcaact tcttcttcac
     5341 cctgcgcgcc gccaagctga aggtctcggt caaggagtac ctgatgcttc tcgaagccat
     5401 ccaggccggc gtgatcgaca acaccgtcga cgagttctac tacctcgcgc gcagctcgct
     5461 cgtcaaggac gaggcgaatt tcgacaagtt cgaccgcgcg ttcgccgcct ac
//
LOCUS       DATMJB010000041         3872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_289953, whole genome shotgun sequence.
ACCESSION   DATMJB010000041 DATMJB010000000
VERSION     DATMJB010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289953"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VNU48_01005"
     CDS             <1..1404
                     /locus_tag="VNU48_01005"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HWI09889.1"
                     /translation="MPCCDVINRGAALYENLVIFATLDAQLVALDQDSGNVVWKEKLE
                     DYKAGYSATAAPIIANGLVLTGVSGGEFGVVGRVEARSAKTGQLVWTRPTVEGHMGYT
                     YDPDGNPKENGISGTRNQSWPGDLWKTGGAATWLGGTFDAKTGLAYFGTGNPAPWNSH
                     LRKGDNLFSCSTVAIDVKTGQIKWHYQGTPNDGWDFDGVNEFVTFDMDGKRVGGKADR
                     NGFFYVNDASTGQLINAFPFVKKITWASSIDLKTGRPNFIAENRPGDPTAGSDGKKGS
                     SIFSAPSFLGGKNQMPMAYSPDTKLFYVPSNEWGMEIWNEPVAYKKGAAYLGAGFTIK
                     TLNDDYIGALRAVDPKTGKIVWEVKNSAPLWGGVLTTGGNLVFWGTPEGYLKAADAKT
                     GKIVWEFQTGSGVVAPPVTWQQGGEQYVSVVSGWGGAVPLWGGEVAKKVNFLEQGGMV
                     WVFKIPKAAEQKVAKAN"
     gene            1471..1791
                     /locus_tag="VNU48_01010"
     CDS             1471..1791
                     /locus_tag="VNU48_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HWI09890.1"
                     /translation="MKDWTRRIFITRTVAGAYAAFAVPATVHATPSRVEEADETAAAL
                     GYKHDTKQVDAQRYPNHSAAQSCANCSFFQGSAADGWAGCAMFGRKHIAATGWCSAWA
                     KKPG"
     gene            complement(1843..>3872)
                     /locus_tag="VNU48_01015"
     CDS             complement(1843..>3872)
                     /locus_tag="VNU48_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HWI09891.1"
                     /translation="RTGLIEQSHLRCAALGLTRVGHLEFEPMMRSDLSVARERNQRLF
                     SHAAPVMEMLFEQIVNTESMIVLTDAQGTILHSVGDNEFLARADKVALSPGVNWAEQS
                     KGTNAIGTALFEERPTLVHGGEHFIHANSFLTCSAAPIFDPRGNMLGVLDVTGDQRSY
                     HQHTMGLVRMSARMIENHWLSDDYGDRLRLHFHSRAEFIGTLLEGIIVVGADGKILGA
                     NRSALDQLDLSGAALRMQSLSSLFGTTISAVFDHFRAPLPAPMRLSQPNGRKFCVSAR
                     INGPMRSMMGGLAIDEAVSVRRVDGAPAVFDAGRSANLGPSQGTSTSRLSGLHYLKTG
                     DPLVEAMVRKVQRVLNRDIPLLILGETGTGKELLARAVHQDSNRAKQAFVAVNCASIP
                     ESLIEAELFGYEDGAFTGARRKGASGRILQASGGTLFLDEIGDMPLALQARLLRVLQE
                     RCVTPLGSLKSIPVDIAVVAATHRDLRKMIEAGAFREDLYYRLNGLVVRLPALRDRCD
                     LDAVARRILLSECPQRTPEISAPVMALFDRYRWPGNIRQLANVLRTAAVMAAGERQIT
                     EQHLSDDFLEDVQHAPGLPMRQAPQAAALPAPPAAPIACDPGDAPGAVSVAAEVAGDR
                     SDRTNRTLGEAEIDMMRAALAAANGNISEASKRLGISRNTIYRKLRWAKPA"
BASE COUNT          642 a         1306 c         1343 g          581 t
ORIGIN      
        1 atgccgtgct gcgacgtgat caaccgcggc gccgcgctgt acgagaacct ggtgatcttc
       61 gcgacgctcg atgcacagct cgtcgcgctc gaccaggaca gcggcaacgt cgtctggaag
      121 gagaagctcg aagactacaa ggccggctac tcggcgaccg ccgcgccgat catcgccaac
      181 ggcctggtgc tcaccggcgt gtcgggcggc gagttcggcg tggtcggccg ggtcgaggcg
      241 cgcagcgcga agaccgggca actggtctgg acgcgcccga cggtcgaagg ccacatgggc
      301 tacacctacg acccggacgg caacccgaag gagaacggca tctccggcac ccgcaaccag
      361 agctggcccg gcgacctgtg gaagaccggc ggcgcggcaa cctggctcgg cggcaccttc
      421 gatgccaaga ccggcctggc ctacttcggc accggcaacc cggcgccgtg gaacagccac
      481 ctgcgcaagg gcgacaacct gttctcgtgc tcgaccgtcg cgatcgacgt gaagaccggc
      541 cagatcaagt ggcactacca gggcacgccg aacgacggct gggacttcga cggcgtcaac
      601 gagttcgtga ccttcgacat ggacggcaag cgtgtcggcg gcaaggccga ccgcaacggc
      661 ttcttctacg tcaacgatgc gagcaccggc cagctgatca acgccttccc gttcgtcaag
      721 aagatcacct gggcgagcag catcgacctg aagaccggcc gccccaactt catcgccgag
      781 aaccggcccg gcgacccgac cgccggcagc gacggcaaga agggcagctc gatcttctcg
      841 gcaccgagct tcctcggcgg caagaaccag atgccgatgg cctacagccc tgacaccaag
      901 ctgttctacg ttccctccaa cgagtggggc atggagatct ggaacgagcc ggtcgcgtac
      961 aagaagggcg ccgcctacct gggcgcgggc ttcacgatca agacgctcaa cgacgactac
     1021 atcggcgcgt tgcgcgcggt cgacccgaag accggcaaga tcgtctggga agtgaagaac
     1081 agcgcgccgc tgtggggtgg cgtgctgacc accggtggca acctcgtgtt ctggggtacg
     1141 cccgagggct acctgaaggc cgccgatgcg aagaccggca agatcgtctg ggagttccag
     1201 accggctcgg gcgtcgtggc gccgccggtc acctggcagc aaggcggcga gcagtacgtg
     1261 agcgtcgtgt cgggctgggg cggtgcggtg ccgctgtggg gcggcgaggt cgcgaagaag
     1321 gtcaacttcc tcgagcaggg cggcatggtg tgggtgttca agatccccaa ggccgctgaa
     1381 cagaaggtcg cgaaggcgaa ctgacgcact tgcccacggg gcgtcgccct gcgccgagcg
     1441 cggagcgacg cccccctctt ggagactgac gtgaaagact ggacccggcg cattttcatc
     1501 acccgaaccg tggcaggcgc ttacgctgca ttcgccgttc ccgccaccgt gcacgcgacg
     1561 ccatcgcgcg tcgaggaggc cgacgagacg gcggccgcgc tcggctacaa gcacgacacc
     1621 aagcaggtcg acgcgcagcg ctacccgaac cacagcgcgg cgcagagctg cgccaactgc
     1681 tcgttcttcc agggcagcgc ggccgatggc tgggccggtt gcgcgatgtt cggccgcaag
     1741 cacatcgccg cgaccggctg gtgcagcgcc tgggccaaga agccgggctg agagctgacg
     1801 accccgaggt cgcctgcggc gcccggcccc cccaagtggc gctcaggcag gcttggccca
     1861 gcgcaacttg cggtagatcg tgttgcggct gatgcccagg cgcttcgatg cctcggaaat
     1921 gttgccgttc gccgcggcga gtgccgcgcg catcatgtcg atctcagctt cgcccagtgt
     1981 gcggttcgtg cggtcgctgc ggtcacccgc gacctcggcc gcgaccgaca cagcgccggg
     2041 cgcgtcgccc gggtcgcacg cgatcggcgc tgccggcggc gccggcagcg ccgcggcctg
     2101 cggtgcctgg cgcatgggca gccctggcgc gtgctgcacg tcctcgagaa agtcgtccga
     2161 cagatgctgc tcggtgatct gccgctcgcc cgcggccatc accgcggcgg tgcgtagcac
     2221 gttggcgagt tggcgaatgt tgccgggcca cctgtagcga tcgaacagcg ccatcaccgg
     2281 cgcgctgatc tcgggcgttc gctgcggaca ttcggagagc aggatgcgcc gcgcgaccgc
     2341 gtcgagatcg cagcggtcgc gcagtgccgg caggcgcacg acgaggccgt tgagccggta
     2401 gtacaggtct tcgcggaacg cgccggcctc gatcatcttg cgcaggtcgc ggtgcgtggc
     2461 cgcgacgacg gcgatgtcga ccgggatcga cttcagactg ccgagcggcg tcacgcagcg
     2521 ctcctgcaac acgcgcagca accgcgcctg cagcgccagc ggcatgtcgc cgatctcgtc
     2581 gaggaacagc gtgccgccgc tggcctgcag gatgcggccg ctcgcgccct tgcggcgtgc
     2641 gccggtgaag gcgccgtctt cgtagccgaa cagctcggcc tcgatcagcg attcggggat
     2701 cgaggcacag ttgacggcga cgaaggcctg cttcgcgcgg ttcgagtcct ggtgcacggc
     2761 acgggcgagc aactccttgc cggtgccggt ttcgccgagg atcagcagcg ggatgtcgcg
     2821 gttcaggaca cgctgcacct tgcgcaccat cgcctcgacc agcgggtcgc cggtcttcag
     2881 gtagtgcagc cccgagagcc ggctcgtcga tgtgccttgc gaaggcccga ggttcgcgga
     2941 tcggccggcg tcgaacactg cgggcgcgcc gtcgacacgg cgcaccgata ccgcctcgtc
     3001 gatcgcgagg ccgcccatca tcgagcgcat cggcccgttg atgcgcgcgc tgacgcagaa
     3061 cttcctcccg ttcggctggc tcaggcgcat cggcgccggc agcggtgcgc ggaagtgatc
     3121 gaacaccgcc gatatcgtgg tgccgaacag gctcgacagg ctttgcatgc gcaaggccgc
     3181 gccgctcagg tcgagttggt cgagcgcgct gcgattcgcg ccgaggatct tgccgtcggc
     3241 gccgaccaca atgatccctt cgagcagcgt gccgatgaac tcggcgcggc tgtggaagtg
     3301 cagccgcagg cgatcgccgt agtcgtcgga gagccagtgg ttctcgatca tgcgtgccga
     3361 catgcgcacc agccccatcg tgtgctggtg atacgagcgc tggtcaccgg tcacgtcgag
     3421 cacgccgagc atgttgccgc gcggatcgaa gatcggcgcg gccgaacagg tcaggaagct
     3481 gttcgcatgg atgaagtgct cgccgccgtg cacgagcgtg ggccgctcct cgaacagcgc
     3541 ggtgccgatc gcattcgtgc ccttcgactg ttcggcccag ttgacgcccg gcgacagcgc
     3601 caccttgtcg gcgcgcgcga gaaactcgtt gtcgccgacc gagtgcagga tcgttccctg
     3661 cgcatcggtc aggacgatca tgctttcggt gttgacgatc tgctcgaaga gcatctccat
     3721 caccggcgct gcgtggctga acaggcgctg gttgcgctcg cgcgccacgc tgaggtcgct
     3781 gcgcatcatc ggctcgaact cgaggtggcc gacgcgcgtc agcccgagcg cggcgcagcg
     3841 caggtgcgac tgctcgatca ggcccgtccg cg
//
LOCUS       DATMJB010000042         3555 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_349042, whole genome shotgun sequence.
ACCESSION   DATMJB010000042 DATMJB010000000
VERSION     DATMJB010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3555
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349042"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VNU48_01020"
     CDS             complement(<1..554)
                     /locus_tag="VNU48_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09892.1"
                     /translation="MSESCSRSLARLVRAAVAIAALWSAAVGAQAQGQALLGVYYGNQ
                     GFNMDEVRAMEAWQGKRHAVVNLFTDWCNRSKNLDNLFKLQLPNVWANGNVPLVSWEP
                     YLCSPSATPSDVEVRAARGDYDAYLNAWADRLKAFVSGPDGSLGTADDRRVYIRLAHE
                     MNGNWYPWGAAVGGNSAASYTLMW"
     gene            complement(671..1291)
                     /gene="rutR"
                     /locus_tag="VNU48_01025"
     CDS             complement(671..1291)
                     /gene="rutR"
                     /locus_tag="VNU48_01025"
                     /inference="COORDINATES: protein motif:HMM:NF011584.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HWI09893.1"
                     /translation="MTKKKAAAAVPNKREAVLSAALGLFSRFGLHGTSVDQVAARAGV
                     SKSNLLYYFANKEELYVSVLRELLEVWLAPLREFSAEQDPQQVIADYIRRKLAVSRDS
                     PEASRLFCLEMVQGAPLLRDELERELRDIVERKSAVIRAWIGAGRLAPVDPEQLLFMI
                     WAATQHYADFAVQVQALTGQTLADPAFFEKTVANVQALVLRGVAPR"
     gene            1457..2731
                     /locus_tag="VNU48_01030"
     CDS             1457..2731
                     /locus_tag="VNU48_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HWI09894.1"
                     /translation="MADIKINGDRLWASLMDMAKIGATPKGGVCRLTLTDLDKQGRDL
                     FVQWCKDAGCSVKVDQMGNIFARRPGRNNNLAPVGTGSHLDSQPTGGRFDGVYGVLAG
                     LEVIRTLNDHGIETERPVEASVWTNEEGSRFAPAMVGSGVYAGVFSLAYGHSRTDQQG
                     KTIGEELKRIGYLGAEPMGNRPVHAFFETHIEQGPILEHEGQTIGVVTDAQGQRWYEL
                     VLTGQDSHAGPTPMGVRRDALLGAAKVVQLVNEIGLKHAPLAVSTVGMLNVFPNSRNV
                     IPGKTFLTVDFRCPDNDELLKMDAELKEGVAKIAAAGRLEHDLKQIFQYDCVHFDESC
                     VQMVRAGAQRLGYSTRDIVSGAGHDACYMSKVTPTAMVFVPCVGGISHNEIEDAKPEW
                     IAAGGNVLFQAMLAKANELPEVFKSNATVAVA"
     gene            2757..>3555
                     /locus_tag="VNU48_01035"
     CDS             2757..>3555
                     /locus_tag="VNU48_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWI09895.1"
                     /translation="MSSILIRGGTVINAEASVRADVLTQDGLIAAVGANLQAPAGATI
                     VDAGGALVMPGGIDPHTHMELPFMGTVTKDDFYTGTAAGVSGGTTMIIDFVIPDPKQR
                     LMDAWKNWNGWAEKAAADYSFHVAVTWWDQSVHDDMGTLVREHGVNSFKHFMAYKNAI
                     MADDEVLVNSFTRALELGALPTVHAENGELVFQMQKRLVEQGITGPEGHPLSRPPAVE
                     AEAAERAIRIAEVLGTPLYIVHVSSQDSVEAIIRARSHGQRVYGEVLA"
BASE COUNT          599 a         1228 c         1203 g          525 t
ORIGIN      
        1 tgccacatca gggtgtagct ggccgcagag ttgccgccga ccgccgcgcc ccacgggtac
       61 cagttgccgt tcatctcgtg cgccagccgg atgtagacgc gccgatcgtc cgcggtgccg
      121 aggctgccgt cagggccgct cacgaaggcc ttgagccggt cggcccaggc gttcaggtag
      181 gcgtcgtagt cgccgcgcgc cgcgcgcacc tcgacatcgc tcggagtcgc cgatggcgag
      241 cacaggtagg gctcccagct gacgagcggc acgttgccgt tggcccagac attgggcaat
      301 tgcagcttga acaggttgtc caggttcttg ctgcggttgc accagtcggt gaacaggttc
      361 accacggcgt ggcgcttgcc ctgccacgcc tccatggcgc gaacctcatc catgttgaag
      421 ccctggttgc cgtagtagac gcccagcagg gcctgaccct gcgcctgcgc gccgacggcc
      481 gcactccaca gggcggcgat cgccaccgcc gcgcgcacca ggcgcgcgag ggagcgggag
      541 cacgactcgg acatcgcgat tcctttcgtt cccgagcgac gcccggacgc gatccagtct
      601 gccccgggtc gcgtcgcctc cgcaaggccc cggaccgagg gttgacatca gaccgcgctg
      661 cacgccggcg ctatcggggc gccacgccgc gcagcaccag cgcctgcacg ttcgccaccg
      721 tcttctcgaa gaacgccggg tccgcgagcg tctgcccggt cagcgcctgc acctgcaccg
      781 cgaagtcggc gtagtgctgg gtcgcggccc agatcatgaa gagcagctgc tcggggtcga
      841 ccggcgcgag tcgcccggcc ccgatccagg cgcggatcac cgccgacttg cgctcgacga
      901 tgtcgcgcag ctcgcgctcg agctcgtcgc gcagcagcgg cgcgccctgc accatctcca
      961 ggcagaacag gcgcgaggcc tcggggctgt cgcgcgagac cgcgagcttg cggcggatgt
     1021 agtcggcgat cacctgctgc gggtcttgtt ccgcactgaa ctcgcgaaga ggggcgagcc
     1081 agacctcgag cagctcgcgc agcacgctga cgtaaagctc ttccttgttc gcgaagtagt
     1141 acagcaggtt gctcttcgac acgccggccc ttgccgcgac ctggtcgacg ctggtgccgt
     1201 gcagcccgaa gcgcgagaac agcccgagcg cggcactcag caccgcctcg cgcttgttcg
     1261 gcaccgctgc ggcggccttc ttcttcgtca ccggcccctc ctgttgtcgc gacggttggc
     1321 ggcgagtctc tcagccggat cggtgtttgt ccatatggtc aaactggcac gccggttgca
     1381 tgctgacgcc ggcagtggac gcacgccgca gatgggtgca taacgtcgtt tcgttgaacc
     1441 acgcaggagc aatgacatgg ctgacatcaa gatcaacggc gaccgcctct gggccagcct
     1501 gatggacatg gccaagatcg gcgcgacccc caagggcggc gtgtgccgcc tcacgctgac
     1561 cgatctcgac aagcagggtc gcgacctgtt cgtgcagtgg tgcaaggacg ccggctgcag
     1621 cgtcaaggtc gaccagatgg gcaacatctt cgcgcgccgc ccggggcgca acaacaacct
     1681 ggcgccggtc ggcaccggca gccacctcga cagccagccc accggcggcc gcttcgacgg
     1741 cgtctacggc gtgctcgccg gtctggaagt gatccgcacc ttgaacgacc acggcatcga
     1801 gaccgagcgg ccggtcgagg cctcggtctg gaccaacgag gaaggctcgc gcttcgcgcc
     1861 ggcgatggtc ggctcgggtg tctacgccgg tgtgttctcg ctggcctacg gccactcgcg
     1921 caccgaccag cagggcaaga ccatcggcga ggagctgaaa cgcatcggct acctcggcgc
     1981 cgagccgatg ggcaatcgcc cggtgcacgc gttcttcgag acccacatcg aacaaggccc
     2041 gatcctcgag cacgagggtc agacgatcgg cgtggtcacc gacgcgcagg gccagcgctg
     2101 gtacgagctg gtgctgaccg gccaggactc gcatgccggg ccgacgccga tgggcgtgcg
     2161 ccgcgacgcg ctgctgggtg ccgcgaaggt ggtgcagctc gtcaacgaga tcgggctgaa
     2221 gcacgcgccg ctggcggtgt cgaccgtggg catgctcaac gtgttcccga actcgcgcaa
     2281 cgtgatcccg ggcaagacct tcctgaccgt ggacttccgc tgccccgaca acgacgagct
     2341 gctgaagatg gacgccgagc tcaaggaagg cgtggcgaag atcgccgccg ccggccggct
     2401 cgagcacgac ctgaagcaga tcttccagta cgactgcgtg cacttcgacg agagctgcgt
     2461 gcagatggtg cgcgccggtg cgcagcgcct gggctacagc acgcgcgaca tcgtctcggg
     2521 cgccggtcac gacgcctgct acatgagcaa ggtcacgccc accgcgatgg tgttcgtgcc
     2581 ctgcgtcggc ggcatcagcc acaacgagat cgaggacgcc aagcccgagt ggatcgcggc
     2641 cggcggcaac gtgctgttcc aggcgatgct cgcgaaggcc aacgaactgc ccgaggtgtt
     2701 caagagcaac gcgaccgtcg cggtcgcgtg accgccctga cctggagtac ccgaagatga
     2761 gcagcatcct gatccgcggc ggcaccgtca tcaatgccga ggccagcgtg agggccgatg
     2821 tgctgacgca ggacggcctg atcgctgccg tcggcgcgaa tctgcaggcg ccggccggcg
     2881 ccacgatcgt cgacgccggc ggcgcgctgg tgatgcccgg cggcatcgac ccgcacaccc
     2941 acatggagct gcccttcatg ggcaccgtca cgaaggacga cttctacacc ggcaccgcgg
     3001 cgggggtttc gggcggcacg acgatgatca tcgacttcgt gatccccgac ccgaagcaac
     3061 gcctgatgga cgcgtggaag aactggaacg gctgggccga gaaggccgcg gccgactaca
     3121 gcttccacgt cgccgtcacg tggtgggacc agtcggtgca cgacgacatg ggcacgctgg
     3181 tgcgcgagca cggcgtgaac agcttcaagc acttcatggc ctacaagaac gcgatcatgg
     3241 ccgacgacga ggtgctggtg aacagcttca cgcgcgcgct cgaactcggc gcgctgccga
     3301 ccgtgcacgc cgagaacggc gagctcgtgt tccagatgca gaagcgtctg gtcgagcaag
     3361 gcatcaccgg ccccgaaggc cacccgctgt cgcgcccgcc ggcggtcgag gccgaggccg
     3421 ccgaacgcgc gatccgcatc gccgaggtgc tgggcacgcc gctctacatc gtccacgtgt
     3481 cgagccagga ttcggtcgaa gcgatcatcc gcgcgcgcag ccacggccag cgcgtctacg
     3541 gcgaggtgct cgcgg
//
LOCUS       DATMJB010000043         2672 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_703998, whole genome shotgun sequence.
ACCESSION   DATMJB010000043 DATMJB010000000
VERSION     DATMJB010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703998"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VNU48_01040"
     CDS             <1..832
                     /locus_tag="VNU48_01040"
                     /inference="COORDINATES: protein motif:HMM:NF041043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HWI09896.1"
                     /translation="GAPMRLQTVPARQGALWVRQGFNAFFKRPLAFAALFATFMFAVF
                     VLALLPYIGALIMLALLPLVTLGFMIATRVAVDGGFPTARVFVDPLRDPAARRNAMIQ
                     LGLVYAAGTFAVMWLSDLVDRGAFEALMDTMPMAQTSPDAVAAKLAAPGLGLGLLLRF
                     GLAGLLSVPFWHAPALVHWDGHGCAKALFSSTLACWRNRGAFAVYSLLWFAVIIAFGM
                     VGSLLFGLLGQPQLFAVAAVPMSLLLATVFYVSLYFTFADCFVASDGGAIAGPVADAA
                     "
     gene            876..1622
                     /locus_tag="VNU48_01045"
     CDS             876..1622
                     /locus_tag="VNU48_01045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013591686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI09897.1"
                     /translation="MKIAIVTGAGSGIGRATSLALLAAGYEVVLAGRRADALNETRAL
                     AGALAEHALSVPTDATDPAAVRALFETTRERFGRLDLLFNNAGTGAPAVPMDELTVEQ
                     WKAVVDINLTGVFLCTQQAFRLMKGQRPRGGRIINNGSISAYAPRPFSAPYTATKHAI
                     TGLTKATSLDGRAHDIACGQIDIGNAASEMTQRMASGVPQANGTMMVEPRMDLKHVAD
                     AVVHMASLPLEANVQFMTVMATKMPFVGRG"
     gene            1742..>2672
                     /locus_tag="VNU48_01050"
     CDS             1742..>2672
                     /locus_tag="VNU48_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09898.1"
                     /translation="MTDARGATLPPSRASLQDFEPLLPAERLVLRAAAASEIAKIGYR
                     RPGAPAPDQTVRAELLAYLARGGGPGATVAARQLQIMGAWVSGRLDLAGASVPLSLWL
                     YRCGFALTPLFDGAHVLGSLSFADCTLPGLRAEGCRIDGDLALSAGSDVEGEVRLTRT
                     RIGHELNCERLGLRGSRSLFVADGTRIGGDVNLCGGVEAVGELRFIGAHIGGDLRASA
                     ARLTADIDPAGARGVALNLDRACIAGSVALDAGFSAGGAVRLQQAEIRGDLDCSGADF
                     DAVGDASWGAGGAGLVLTRAQVGGSLSLQRLGTP"
BASE COUNT          317 a          982 c          965 g          408 t
ORIGIN      
        1 aggggctcca atgagacttc agaccgttcc ggcccgccag ggtgcgttgt gggtgcgcca
       61 gggcttcaac gcgttcttca agcgcccgct cgcgttcgcc gcgttgttcg cgaccttcat
      121 gttcgcggtg ttcgtgctcg cgctgctgcc gtacatcggc gcgctgatca tgctggcgct
      181 gctgccgctg gtgacgctgg gcttcatgat cgcgacccgc gtcgcggtcg atggcggctt
      241 cccgaccgcg cgcgtgttcg tcgacccgct gcgcgacccc gcggcgcgtc gcaacgcgat
      301 gatccagctc ggcctggtct acgcggccgg gaccttcgcc gtgatgtggc tcagcgacct
      361 cgtcgatcgc ggcgcgttcg aggcgctgat ggacacgatg ccgatggccc agacctcgcc
      421 cgacgcggtc gcggccaagc tcgccgcccc cgggctcggc ctggggctgc tgctgcgctt
      481 cgggctggcg gggctgctgt cggtgccgtt ctggcacgcg ccggcgctgg tgcactggga
      541 cggtcacggc tgcgccaagg cgctgttctc gagcacgctg gcctgctggc gcaaccgcgg
      601 cgcgttcgcg gtctacagcc tgctgtggtt tgccgtcatc atcgcgttcg gcatggtcgg
      661 cagcctgctg ttcgggctgc tcggccagcc gcagctgttc gcggtcgccg cggtgccgat
      721 gtcgctgctg ctcgccacgg tgttctacgt ctcgctgtac ttcacgttcg ccgattgctt
      781 cgtcgccagc gatggcggcg cgatcgccgg gcccgtcgcc gatgcggcct gacgcgccgc
      841 cggcccctgt tcgacagccc acggaggaag ccctcatgaa gatcgccatc gtcaccggtg
      901 cgggcagcgg catcggccgc gccacctcgc tcgcgctcct cgcggccggc tacgaggtcg
      961 tgctcgccgg ccgccgtgcc gacgcgctga acgagacgcg cgcgctggcc ggcgcgctgg
     1021 ccgagcatgc gttgtcggtg ccgaccgacg cgaccgatcc ggccgcggtg cgcgcgctgt
     1081 tcgagaccac gcgcgaacgc ttcgggcgcc tcgacctgct gttcaacaac gccggcaccg
     1141 gcgccccggc ggtgccgatg gacgaactca cggtcgagca gtggaaggcc gtcgtcgaca
     1201 tcaacctgac cggcgtgttc ctgtgcacgc agcaggcgtt caggctgatg aagggccagc
     1261 gcccgcgcgg cgggcgcatc atcaacaacg gctcgatctc ggcctacgcg ccgcggccgt
     1321 tcagcgcgcc atacaccgcg accaagcacg cgatcaccgg cctgaccaag gccacctcgc
     1381 tcgacggccg tgcacacgac atcgcctgcg gccagatcga catcggcaac gccgccagcg
     1441 agatgacgca gcgcatggcg agcggcgtgc cgcaggccaa cggcacgatg atggtcgagc
     1501 cgcgcatgga cctgaagcac gtcgcggatg cggtcgtgca catggcgagc ctgccgctcg
     1561 aggcgaacgt ccagttcatg acggtgatgg cgaccaagat gccgttcgtc ggccgcggct
     1621 gatcccgacc ccgcaagcgc ggggttgtgt cgagttgtaa cgcaagccgc ttcggccgca
     1681 gaatcgcggt catgccgacg ccgcctgccc tgttgtcgcc cctgcccgca ctggtgccgc
     1741 ggtgacggac gcacgcggcg ccacgctgcc cccgtcccgc gcgtcgctgc aggacttcga
     1801 gcccttgctg ccggccgagc gcctcgtgct gcgggccgcg gccgccagcg agatcgcgaa
     1861 gatcggctac cgccgcccgg gcgcaccggc gcccgatcag accgtgcgcg ccgaactgct
     1921 cgcctacctg gcgcgcggcg gcggcccggg cgcgaccgtg gccgcgcgcc agctgcagat
     1981 catgggtgcg tgggtcagcg gccgcctcga tctggccggc gcgagcgtgc cgctgagcct
     2041 gtggctgtac cgctgcggct tcgcgctgac gccgctgttc gacggcgcgc acgtgctggg
     2101 cagcctgagc ttcgccgact gcacgctgcc gggcctgcgc gccgagggct gccgcatcga
     2161 tggcgacctc gcgctcagcg ccggcagcga cgtcgagggc gaggtccggc tgacgcgcac
     2221 gcgcatcggc cacgagctga actgcgagcg cctgggcttg cgcggttcgc gcagcctgtt
     2281 cgttgccgac ggcacgcgga tcggcggcga cgtgaacctg tgcggcggcg tcgaggcggt
     2341 gggcgagctg cgcttcatcg gcgcgcacat cggcggcgac ctgcgcgcca gcgccgcgcg
     2401 cctgaccgcc gacatcgacc ccgccggcgc gcgcggcgtc gcgctgaacc tggaccgcgc
     2461 gtgcatcgcc ggcagcgtcg cgctcgatgc cggcttctcg gcgggcggcg cggtgcggct
     2521 gcagcaggcc gagatccgcg gcgacctcga ctgcagcggc gccgatttcg acgccgtcgg
     2581 cgacgccagc tggggcgccg gcggggccgg cctggtgctg acgcgtgccc aggtcggcgg
     2641 cagcctgtcg ctgcagcgcc tgggcacgcc gc
//
LOCUS       DATMJB010000044         2988 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_289648, whole genome shotgun sequence.
ACCESSION   DATMJB010000044 DATMJB010000000
VERSION     DATMJB010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289648"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /gene="obgE"
                     /locus_tag="VNU48_01055"
     CDS             <1..879
                     /gene="obgE"
                     /locus_tag="VNU48_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWI09899.1"
                     /translation="ARRGENGRGSDQFGAAADDIVLRMPIGTVITDLETNELVAELLV
                     PDERVLIVKGGDGGFGNLHFKTSTNRAPRQKTPGWPGEQKKLRLELKVLADVGLLGMP
                     NAGKSSLITAISNARPKIADYPFTTLHPNLGVVRVGPEQSFVVADIPGLIEGASEGAG
                     LGHLFLRHLQRTHLLLHLVDFAPFDDAIDPVAQAKAIVAELKKYDKALYDKPRWLVLN
                     KLDMVPIEERDKRVKDFVKRFKWKGPVFQISALTHEGCEPLTRAIYKHVAALKNVVPD
                     DPDPRFDLAPAGSATP"
     gene            876..1994
                     /gene="proB"
                     /locus_tag="VNU48_01060"
     CDS             876..1994
                     /gene="proB"
                     /locus_tag="VNU48_01060"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWI09900.1"
                     /translation="MSTALQRARRIVVKVGSSLVTNEGRGVDADAVGNWCRQMAVLAA
                     QGREVVMVSSGAIAEGMKRLGWSMRPKEVHELQAAAAVGQMGLAQIYESKLREHGMGS
                     AQVLLTHADLADRERYLNARSTLLTLLSYKVIPVINENDTVVVDEIKFGDNDTLGALV
                     ANLVEADALVILTDQRGLYSADPRKEPDARFIAEARAGAPELERMAGGVGSSLGRGGM
                     LTKILAAKRAASSGASTVIAYGRETDVLIRLAGGESIGTALIAPTQKLAARKQWMADH
                     LQLRGAVVVDAGAARKLREEGKSLLPIGVTEVQGEFHRGDVIAVRGPAGDELARGLAN
                     YGSGEARLIARRPSNDIEKLLGYIAEPELIHRDNMVLA"
     gene            complement(2093..2686)
                     /locus_tag="VNU48_01065"
     CDS             complement(2093..2686)
                     /locus_tag="VNU48_01065"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HWI09901.1"
                     /translation="MLDREGFRPNVGIVLLNQRNQVFWGKRIRTHSWQFPQGGIKYGE
                     TPEQAMYRELHEEVGLVPEHVRIVARTRDWLRYEVPDHFIRRDARGHYRGQKQIWFLL
                     QLVGRDSDMNLRATDHPEFDAWRWNEYWVPLDLVIEFKRDVYQMALTELARFLPRGTH
                     HNRYLRSGIRPHHRDDAEQPPSIEPGAALPFVGKLPD"
     gene            2857..2988
                     /locus_tag="VNU48_01070"
                     /pseudo
     CDS             2857..2988
                     /locus_tag="VNU48_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897803.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
BASE COUNT          520 a          999 c         1007 g          462 t
ORIGIN      
        1 gcgcgtcgcg gcgagaacgg acgcggctcc gaccagttcg gtgccgcggc cgacgacatc
       61 gtgctgcgca tgccgatcgg caccgtgatc accgatctcg agacgaacga actggtcgcc
      121 gaactgctgg tgcccgacga acgagtgctg atcgtgaaag gtggcgacgg cggcttcggc
      181 aacctgcact tcaagaccag cacgaaccgt gcgccgcgcc agaagacgcc cggttggccg
      241 ggcgagcaga agaagctcag gctcgaactg aaggtgctcg ccgatgtcgg cctgctcggc
      301 atgcccaatg ccggcaagtc ctcgctgatc accgcgatct cgaacgcgcg cccgaagatc
      361 gccgactacc cgttcacgac cctgcacccg aacctgggcg tggtgcgcgt tgggcccgag
      421 cagagcttcg tggtggcgga cattccgggg ctgatcgagg gcgcatccga aggtgcgggc
      481 ctgggccacc tgttcctgcg ccacctgcag cgcacccatc tgctgctgca cctcgtcgac
      541 ttcgccccct tcgacgacgc gatcgacccc gtcgcgcagg ccaaggcaat cgttgccgaa
      601 ctgaagaagt acgacaaggc gctgtacgac aagccgcgtt ggctggtgct caacaagctc
      661 gacatggtgc cgatcgaaga gcgcgacaag cgcgtcaagg acttcgtcaa gcgcttcaag
      721 tggaagggcc cggtgttcca gatttcggcg ctgacccacg agggctgcga gccgctgacc
      781 cgcgcgatct acaagcacgt cgccgcactg aagaacgtcg ttccggacga ccccgatccg
      841 cgcttcgacc ttgcacccgc gggcagcgcc acgccatgag cacggcgctg cagcgtgcgc
      901 gccgcatcgt cgtcaaggtc ggctccagtc tggtgacgaa cgagggccgt ggcgtcgacg
      961 ccgacgcggt cggcaactgg tgccgccaga tggcggtact ggcggcgcag ggtcgcgagg
     1021 tcgtgatggt ctccagcggc gcgatcgccg agggcatgaa acgcctcggg tggtcgatgc
     1081 ggccgaagga agttcacgag cttcaagcgg ccgccgcggt tggccagatg ggcctggcgc
     1141 agatctacga atcgaagctg cgcgagcacg gcatgggcag cgcgcaggtg ctgctcacgc
     1201 atgccgacct cgccgaccgc gagcgttacc tgaatgcgcg ctcgaccctg ctgaccttgc
     1261 tgtcgtacaa ggtgattccg gtgatcaacg agaacgatac cgtcgtcgtc gacgaaatca
     1321 agttcggcga caacgacacg ctcggcgcgc tggtggccaa cctggtcgaa gccgatgcgc
     1381 tcgtgatcct gacggaccag cgcggcctgt attcggccga cccgcgcaag gagccggacg
     1441 cgcgcttcat cgccgaagcc cgtgccggcg cgcccgagct cgaacggatg gccggcggcg
     1501 tcgggtcgag cctggggcgc ggcggcatgc tcacgaagat cctcgccgcg aagcgcgcgg
     1561 ccagcagtgg cgccagcacc gtgatcgcct acgggcgcga aaccgatgtg ctgatccggc
     1621 tggccggcgg tgaatcgatc ggcaccgcgc tgatcgcgcc gacgcagaag ctcgccgcgc
     1681 gcaagcagtg gatggccgat cacctgcagc tgcgtggtgc cgtggtcgtc gacgccgggg
     1741 cggcacgcaa actgcgcgag gaaggcaaga gcctcttgcc aatcggcgtg accgaggtgc
     1801 agggtgagtt ccaccgcggc gacgtgatcg cggtgcgcgg cccggccggc gacgagctgg
     1861 cgcgcgggct tgcaaactac ggcagcggcg aggcgcggct gatcgcgcgc cggccgtcga
     1921 acgacatcga gaagctgctg ggctacatcg ccgagcccga gctgatccac cgcgacaaca
     1981 tggttctggc ctagaaatga cgacccctgg gcgcctttgg caccgtctcc cgtggggaac
     2041 tcaggccgct tggggcggcc ggacgctggc ctgaggaacc gccgcttcga gatcagtcgg
     2101 gcagcttgcc gacgaacggc aacgcggcac ccggctcgat cgacggcggc tgctccgcgt
     2161 cgtcgcggtg gtgcggccgg atgcccgatc gcaggtagcg gttgtggtgc gtgccgcgcg
     2221 gcaggaagcg tgcgagttcg gtcagcgcca tctggtacac gtcgcgcttg aactcgatca
     2281 cgaggtcgag cggcacccag tactcgttcc agcgccaggc gtcgaactcg gggtggtcgg
     2341 tggcgcgcag gttcatgtcg ctgtcgcgcc cgacgagctg caacaggaac cagatctgct
     2401 tctggccgcg gtagtggccg cgcgcgtcgc ggcgaatgaa atgatcgggg acttcgtagc
     2461 gcaaccagtc gcgtgtgcgc gcgacgatgc gcacatgctc gggcacgagg cccacctctt
     2521 cgtggagctc gcggtacatc gcctgctcgg gcgtctcgcc gtatttgatg cccccttgcg
     2581 ggaactgcca ggagtgggtg cggatccgct tgccccaaaa cacctggttt ctctggttga
     2641 gcaggacgat gccgacgttg ggtctgaacc cttcacggtc gagcataatc aaccccaatt
     2701 cattcgttga attcattatt acaccggtgt gacgggatac aacccctgcg gtttcccgca
     2761 gccctcgggc cacgcgaccg gtgccgcgct cagccgcaca cccacacgcg cccttccatg
     2821 aaagcatccc ggttcttcat ctccacgctc aaagaagacg agccgctgtc caacctcgac
     2881 gccaagctgc gcgtcgagat gcgcgcggcc gtctcgcgcc tgcagcagcg cctcggcacg
     2941 accaccgtct acgtcaccca cgaccagacg gaagccatga ccctcggc
//
LOCUS       DATMJB010000045         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_827694, whole genome shotgun sequence.
ACCESSION   DATMJB010000045 DATMJB010000000
VERSION     DATMJB010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827694"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VNU48_01075"
     CDS             <1..1024
                     /locus_tag="VNU48_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWI09902.1"
                     /translation="TGDIPAEWQHRWRTAGVQWRVFSPARGWRLLLPKRWRRMHRKLC
                     VIDGETGFCGGINLLDDYFDPNYGVLEQPRFDFAARVTGPLVADMHETMARLWARLQV
                     AREAQQLDFGAALQAVLAAAQAGTDMGDERMEGEVEDFGAADADEGALAALVLRDNVR
                     FRRSIEGNYRLAIALARSEILIANAYFVPGVQLQRALLRAARRGVRITLLLQGKYEYF
                     MQYHASRAVYAVMLDAGIEIIEYDESFLHAKVAVMDAAGGALAIVGSSNLDPLSLLLA
                     REANVFVRNDAFAGELRAHLVEAIAKGRRVAPDAAAHWGIVSRALNWVAYAMMRAALL
                     VTGKRY"
     gene            complement(1032..1433)
                     /locus_tag="VNU48_01080"
     CDS             complement(1032..1433)
                     /locus_tag="VNU48_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HWI09903.1"
                     /translation="MSLVTAPTAAPPSPFDDPMSTAQILFAFRGRVPRRVFWFYGVLG
                     PLLVSVMAEMLLGIVGVSERGAELLTTALLVWPCAAVGVKRWHDRDKSGWWVLVWLIP
                     LIGWLWTLIGNGALRGSVGPNRFGDDLTGKF"
     gene            1497..>2064
                     /locus_tag="VNU48_01085"
     CDS             1497..>2064
                     /locus_tag="VNU48_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HWI09904.1"
                     /translation="MNLVDDLVVLRPEGLYCPPGDFYIDPWRPVERAVITHAHADHAR
                     TGHAHYLAAAPGEGVLRARLGAVDLQTLAYGAAVDHFGVRVSLHPAGHVLGSAQVRLE
                     HRGQVWVASGDYFVAGAADDPLEANATCTPFEPVRCHCFITESTFGLPIYRWQPQAEL
                     FAAIDDWWRGNAEAGRASLILGYSFGKAQ"
BASE COUNT          303 a          744 c          734 g          283 t
ORIGIN      
        1 cactggcgac atccccgccg aatggcagca ccgctggcga acggccggcg tgcaatggcg
       61 cgtcttcagc ccggcccgcg gctggcgctt gctgctgccc aagcgctggc gccgcatgca
      121 ccgcaagctg tgcgtcatcg acggcgagac cggcttctgc ggcggcatca acctgctcga
      181 cgactacttc gacccgaact acggcgtgct cgagcagccg cgcttcgatt tcgcggcgcg
      241 cgtcaccggc ccgctggtgg ccgacatgca cgagacgatg gcccgcctgt gggcgcgcct
      301 gcaggtcgcg cgcgaggccc agcaactgga tttcggtgcg gcgctgcagg cggtgctggc
      361 cgcggcgcag gccggcaccg acatgggcga cgagcgcatg gaaggcgagg tcgaggactt
      421 cggcgcggcc gacgcggacg aaggcgcgct cgcggcgctg gtgctgcgcg acaacgtgcg
      481 cttccggcgc agcatcgaag gcaactaccg tctcgcgatc gcgctggcgc gcagcgagat
      541 cctgatcgcc aacgcctact tcgtcccggg cgtgcagctg cagcgtgcgc tgctgcgcgc
      601 ggcgcggcgc ggcgtgcgca tcacgctgct gctgcagggc aagtacgagt acttcatgca
      661 gtaccacgcg agccgcgcgg tctacgcggt gatgctcgat gccggcatcg agatcatcga
      721 atacgacgag agcttcctgc acgccaaggt cgcggtgatg gacgccgccg gcggcgccct
      781 ggcgatcgtc ggctcgtcga acctcgaccc gctgagcctg ctgctggcgc gcgaggccaa
      841 cgtcttcgtg cgcaacgacg cgttcgcggg cgagctgcgt gcgcacctgg tcgaggcgat
      901 cgcgaaaggc cggcgcgtcg cgccggacgc ggccgcgcac tgggggatcg tgtcgcgcgc
      961 tctgaactgg gtcgcctacg cgatgatgcg cgcggcgctg ctcgtgaccg gcaagcgcta
     1021 ctagcccggc atcagaactt gccggtcagg tcgtcgccga agcggttcgg gccgacgctg
     1081 ccgcgcagcg cgccgttgcc gatcagcgtc cagagccagc cgatcagcgg gatcagccag
     1141 accagcaccc accagcccga cttgtcgcgg tcgtgccagc gcttcacgcc gaccgccgcg
     1201 cacggccaca cgagcagcgc ggtcgtcagc agttcggcgc cgcgctcgct gacgccgacg
     1261 atgcccagca gcatctccgc catcaccgac accagcagcg gcccgagcac gccgtagaac
     1321 cagaacaccc tgcgcggcac gcggccgcga aaggcgaaca ggatctgcgc cgtcgacatc
     1381 gggtcgtcga agggcgaggg cggggcggcg gtcggtgcgg tgacgaggga catcgggcgg
     1441 gacgcgtgaa tgcgccaggc acgatcgtag gcgatcacgt cgccgtacga tgccgcatga
     1501 acctggtcga cgacctcgtc gtgctgcgcc ccgaaggcct gtactgcccg ccaggggact
     1561 tctacatcga cccgtggcgg ccggtcgagc gcgcggtgat cacgcacgcc catgccgacc
     1621 acgcgcgcac cgggcacgcg cactacctgg ccgccgcacc cggcgaaggc gtgctgcgtg
     1681 cgcgcctggg cgcggtcgac ctgcagacgc ttgcctacgg cgccgcggtc gaccacttcg
     1741 gcgtgcgcgt ctcgctgcac ccggccggtc acgtgctcgg gtcggcgcag gtgcggctcg
     1801 agcaccgcgg gcaggtctgg gtcgcttcgg gcgactactt cgtcgccggc gcggccgacg
     1861 atccgctcga ggccaacgcg acctgcacgc cgttcgagcc ggtgcgctgc cactgcttca
     1921 tcaccgagtc gaccttcggc ctgccgatct accgctggca gccgcaggcc gagctgttcg
     1981 ccgcgatcga cgactggtgg cggggcaacg ccgaggccgg ccgcgccagc ctgatcctcg
     2041 gctacagctt cggcaaggcg cagc
//
LOCUS       DATMJB010000046         4315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_644130, whole genome shotgun sequence.
ACCESSION   DATMJB010000046 DATMJB010000000
VERSION     DATMJB010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644130"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VNU48_01090"
     CDS             <1..354
                     /locus_tag="VNU48_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HWI09905.1"
                     /translation="ASREDHIVPWEGAYRNTQVLTGKTRFVLGASGHIAGVINPPAKG
                     KRSHWIGAGAALPAQAQDWFDNATEHPGSWWTDWTDWLKGHAGKQIAAPKAYGNREHK
                     TIEAAPGRYVKQKAV"
     gene            410..1591
                     /locus_tag="VNU48_01095"
     CDS             410..1591
                     /locus_tag="VNU48_01095"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWI09906.1"
                     /translation="MTTDIVIVAAARTAVGKFGGTLAKTPAPELGAAVVVDLLRRAKL
                     SGDQINELIFGQVLTAGSGQNPARQTVIKSGLPQGVPALTINAVCGSGLKAVMLAAQA
                     IRDGDSEIVIAGGQESMSLSPHVLPGSRDGMRMGEWKMVDSMVTDGLFDVYNKYLMGV
                     TAENVAKKYDITREKQDALALGSQQKAAAAQEAGRFKDEIVPFSIAQKKGDPIVFAAD
                     EYINKKTNAEALAGLRPAFDKAGTVTAGNASGLNDGAAGVVLMTAQKAQALGLKPLAR
                     IASYATVALDPSIMGMGPVPASQKALARAGWKAKDLDLLEINEAFAAQACAVNNEMGW
                     DTSKVNVNGGAIAIGHPIGASGCRILVTLLHEMQRRDAHKGIASLCIGGGMGVALCVE
                     R"
     gene            1721..2458
                     /gene="phbB"
                     /locus_tag="VNU48_01100"
     CDS             1721..2458
                     /gene="phbB"
                     /locus_tag="VNU48_01100"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HWI09907.1"
                     /translation="MNQKIAYITGGMGGIGTTMCQRLHRDGFKVVAGCGPSRDHVKWL
                     GEQAALGYTFYASVGNVGDWDSTVAAFQKVKAEHGPIDVLVNNAGITRDGMFRKMTRA
                     DWDAVIETNLTSLFNVTKQVIDEMVERGWGRIINISSVNGEKGQFGQTNYSAAKAGMH
                     GFTMALAQEVASKGVTVNTVSPGYIATDMVKAIRQEVLDKIIAGIPVKRLGTPGDIAS
                     MVSWVASDESGFATGADFSVNGGLHMG"
     gene            complement(2632..2835)
                     /locus_tag="VNU48_01105"
                     /pseudo
     CDS             complement(2632..2835)
                     /locus_tag="VNU48_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345131.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
     gene            complement(2838..3893)
                     /gene="mnmH"
                     /locus_tag="VNU48_01110"
     CDS             complement(2838..3893)
                     /gene="mnmH"
                     /locus_tag="VNU48_01110"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HWI09908.1"
                     /translation="MLSTINAADALARLDEFGTLIDARSESEFALDRLPGAVNWPSLT
                     DAQRIVVGTEYVQVSPFLAKKRGAALVARNIADHIEREVLDKPKDWRPLVYCWRGGSR
                     SGALATVLDQIGFRVHRLEGGYQAYRRAVIAALETLPARFDYRVICGSTGAGKSRLLQ
                     VLRAQGAQVLDLEALANHRGSVLGLVPGQPQPGQKQFESRVWDALRRFDAARPVYVES
                     ESKKVGDLRVPLLLIERMRAAPCIRLELALDARVRLLLEDYAFFVTDTPAFCARLDAL
                     RTIRGNAVVNAWQEAARAGHTAEVVRDLLVAHYDPIYAQSMQRNFAGASALLLDVAWD
                     GGDASLQDTAAAALRAG"
     gene            complement(3918..>4315)
                     /locus_tag="VNU48_01115"
     CDS             complement(3918..>4315)
                     /locus_tag="VNU48_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWI09909.1"
                     /translation="NSSSYETHYRDLILFVENQLALALADAGPLPEPGRGRISRRVRS
                     FPVTPRITLRPDERAQHWLLGVSASDRSGLLFSIARVLARHHINLQLAKITTLGERVE
                     DTFLIDGPGLQQNKVQLQIETELLDALAP"
BASE COUNT          738 a         1491 c         1509 g          577 t
ORIGIN      
        1 gcctcgcgcg aggaccacat cgtgccgtgg gagggcgcgt accggaatac gcaggtgctc
       61 acgggcaaga cgcgcttcgt gctcggcgcc tcggggcaca tcgccggcgt gatcaacccg
      121 ccggccaagg gcaagcgcag ccactggatc ggcgcgggcg ccgcgttgcc ggcgcaggcc
      181 caggattggt tcgacaacgc gaccgaacac cccggcagct ggtggaccga ctggaccgac
      241 tggctgaagg gccacgccgg caagcagatc gccgcgccga aggcctacgg caaccgcgag
      301 cacaagacca tcgaggcggc gcccggccgc tacgtgaagc agaaggccgt ctgagcgccc
      361 acgtttttcg actacctttt cattttcaac ctcgaacctg gagattccca tgaccaccga
      421 catcgtgatc gtcgccgccg cccgcaccgc cgtgggcaag ttcggcggca ccctggccaa
      481 gacccccgcg cccgaactcg gcgcggccgt cgtcgtcgac ctgctgcgcc gcgcgaagct
      541 cagcggcgac cagatcaacg aactcatctt cggccaggtg ctgaccgccg gctcgggcca
      601 gaacccggcg cgccagaccg tcatcaagag cggcctgccg caaggcgtac cggcactcac
      661 gatcaacgcc gtctgcggct ccggcctgaa ggccgtgatg ctggccgcgc aggcgatccg
      721 cgacggcgac tccgagatcg tcatcgccgg cgggcaagag agcatgagcc tgtccccgca
      781 cgtgctgccg ggttcgcgcg acggcatgcg catgggcgag tggaagatgg tcgactcgat
      841 ggtcaccgac ggcctgttcg acgtgtacaa caagtacctc atgggcgtca ccgccgaaaa
      901 cgtcgcgaag aagtacgaca tcacgcgcga gaagcaggac gcgctcgcgc tcggctcgca
      961 gcagaaggcg gccgccgcgc aagaggccgg caggttcaag gacgagatcg tgccgttcag
     1021 catcgcgcag aagaagggcg accccatcgt cttcgcggcc gacgagtaca tcaacaagaa
     1081 gaccaatgcc gaagcgctgg ccgggctgcg tccggcgttc gacaaggccg gcacggtcac
     1141 cgccggcaac gcctcgggcc tgaacgacgg cgccgccggt gtcgtgctga tgactgcgca
     1201 aaaggcccag gcgctcggcc tgaagccgct tgcgcgcatc gccagctatg ccaccgtcgc
     1261 actcgacccc agcatcatgg gcatgggccc ggtgccggcc tcgcagaagg ccctggcacg
     1321 cgccggctgg aaggccaagg accttgacct gctcgagatc aacgaagcct tcgccgcgca
     1381 ggcctgcgcc gtcaacaacg agatgggctg ggacaccagc aaggtcaacg tcaacggcgg
     1441 cgcgatcgcg atcggtcacc cgattggtgc gtccggctgc cgcatcctgg tgacgctgct
     1501 gcacgaaatg cagcgccgcg acgcgcacaa gggcatcgcc tcgctgtgca tcggcggtgg
     1561 catgggcgtt gcactctgcg tcgagcgctg agcagcgcat cggcgccggg cgggtgaccg
     1621 ccccttgaat caggccgggg cgtgcgcgcc tcggcggacg cacgcgcgag tcggatggcc
     1681 gcgcgtcacg cgtttcggcc agaccacagg gagcaaccaa atgaatcaaa agatcgcata
     1741 catcaccggc ggcatgggcg gcatcggcac gacgatgtgc cagcgcctgc accgggacgg
     1801 cttcaaggtc gtcgccggct gcgggccgag ccgcgaccac gtcaaatggc tgggcgagca
     1861 ggccgcgctc ggctacacct tctacgcctc ggtcggcaac gtcggcgatt gggactcgac
     1921 ggtggcggcg ttccagaagg tcaaggccga gcacgggccg atcgatgtgc tcgtcaacaa
     1981 cgccggcatc acccgcgacg gcatgttccg caagatgacc cgcgccgact gggacgcggt
     2041 gatcgagacc aacctgacga gcctgttcaa cgtcaccaag caggtcatcg acgagatggt
     2101 cgagcgcggc tgggggcgga tcatcaacat cagctcggtg aacggcgaga agggccagtt
     2161 cggccagacc aactactccg ctgcgaaggc cggcatgcac ggcttcacga tggcgctggc
     2221 gcaggaagtc gcgagcaagg gcgtgaccgt caacaccgtg agccccggct acatcgccac
     2281 cgacatggtc aaggcgatcc gtcaggaggt gctcgacaag atcatcgccg gtatcccggt
     2341 caagcggctg ggcacgccgg gcgacatcgc ctcgatggtg tcgtgggttg cgtcggacga
     2401 atcggggttt gcgaccgggg cggacttctc tgtgaacggc gggctgcaca tggggtagag
     2461 cgcgaagcga agggcgaggg gccccgcggg gccacgaagg ggccacttcg tagcccttgg
     2521 ggatcgacca gcgcagcgat cggcgagtga cccgggctcg atgcccgtgt cgcgagagct
     2581 gcgcggcagc gactacatca gcttgccgac gctgaacagc gtcagcaacc ccagcagcgt
     2641 cggcccgagc gcgccggcga cccaggcgcc gaccagcccg gcgctcgcgt gattcgccaa
     2701 cgtggcgacg aggatgccca gcacgatggg caccggccgg cgaaacctga ccgccagcag
     2761 catcgccagc agctgggtct tgtcgccgat ctcgccgagc gcgacgacgc cgaccgagac
     2821 caggaaggcg tccaagctca gccggcccgc agcgccgccg cggccgtgtc ctgcaggctg
     2881 gcgtcgccgc cgtcccacgc gacgtccagc agcagcgccg atgcgccggc gaagttgcgc
     2941 tgcatcgact gcgcgtagat cgggtcgtag tgggccacca gcaggtcgcg cacgacctcg
     3001 gccgtgtgcc cggcgcgcgc cgcctcctgc caggcgttca cgaccgcgtt gccgcggatc
     3061 gtgcgcagcg cgtccaggcg tgcgcaaaag gccggcgtgt cggtgacgaa gaaggcgtag
     3121 tcctcgagca gcaggcgcac gcgcgcgtcc agcgcaagct cgagccggat gcagggcgcg
     3181 gcgcgcatgc gctcgatcag cagcagcggc acgcgtaggt cgccgacctt cttgctctcg
     3241 ctttccacgt agaccgggcg cgccgcatcg aagcgccgca gcgcgtccca gacgcgcgac
     3301 tcgaactgct tttgccccgg ctgcggctgg cccggcacga ggccgagcac cgagccgcgg
     3361 tggttcgcga gcgcctcgag gtcgagcacc tgcgcgccct gcgcgcgcag cacctgcagc
     3421 agccggctct tgccggcgcc ggtgctgccg cagatcacgc ggtagtcgaa gcgcgccggc
     3481 agggtttcga gcgccgcgat caccgcgcgc cgataggcct ggtagccgcc ctcgagccgg
     3541 tgcacgcgaa agccgatctg gtcgagcacg gtcgccagcg cgccgctgcg cgagccgccg
     3601 cgccagcagt agacgagcgg gcgccagtcc ttgggcttgt ccagcacctc gcgttcgatg
     3661 tggtccgcga tgttgcgcgc caccagcgcg gcgccgcgct tcttcgcgag gaagggcgag
     3721 acctgcacgt actcggtgcc gacgacgatg cgctgcgcat cggtcagcga cggccaattg
     3781 accgcgccgg gcagccggtc cagcgcgaat tcgctttcgg agcgcgcgtc gatcagcgtg
     3841 ccgaattcat ccaggcgcgc gagcgcatcg gccgcgttga tcgtcgacag catcgcgtcg
     3901 gcagcgtcgc gcgcggttca gggcgcgagc gcgtccagca gctcggtctc gatctgcagc
     3961 tgcaccttgt tctgctgcag gccggggccg tcgatcagga aggtgtcctc gacgcgctcg
     4021 cccagcgtcg tgatcttcgc cagctgcagg ttgatgtggt ggcgcgcgag cacgcgcgcg
     4081 atcgagaaca gcaggccgga ccggtcgctc gccgagacgc cgagcagcca gtgctgcgcg
     4141 cgctcgtcgg gccgcagggt gatgcgcggc gtgaccggga acgagcgcac gcgccgcgag
     4201 atgcgcccgc gccccggctc gggcagcggg ccggcgtcgg ccagcgccag cgcgagctgg
     4261 ttctcgacga acaggatcag gtcgcggtag tgcgtctcat agctcgaact gttga
//
LOCUS       DATMJB010000047         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_762852, whole genome shotgun sequence.
ACCESSION   DATMJB010000047 DATMJB010000000
VERSION     DATMJB010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762852"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VNU48_01120"
     CDS             complement(<1..223)
                     /locus_tag="VNU48_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWI09910.1"
                     /translation="MKMVTAIVKPFKLDEVREALSAIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAVDDAIVDRVI"
     gene            complement(299..1105)
                     /locus_tag="VNU48_01125"
     CDS             complement(299..1105)
                     /locus_tag="VNU48_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HWI09911.1"
                     /translation="MNKSILLLAITSTLGTAAYAEDPPPAPPFTANISLTTNYKYRGQ
                     DQGSLTPTKAMRPAVQGGFDWTMNGFYVGNWNSSIGWIPDSLGGSSIEMDFYGGYRGE
                     IVKDVGYDVGVLRYQYIGSGFANTTEIYGSVSYGPASLKYSHTVSEDYFGFAGAAFGS
                     GFKGRGTGYLDLSGNFPVMDVLTINAHVGYTRFSSDIRDNIPDTNGGTIPNYYDYKLG
                     VTYDMSKFAGAGVTLAGAVVGASKKESFKYSSDGQSVNKARFIVTLTKTM"
     gene            1348..>2505
                     /locus_tag="VNU48_01130"
     CDS             1348..>2505
                     /locus_tag="VNU48_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HWI09912.1"
                     /translation="MSLAVIHSRALHALEAAEVTVEVHLSNGLPSFTLVGLADTEVKE
                     ARERVRAAIQNSGLEFPHNKRITVNLAPADLPKESGRFDLPIALGILAASGQIDAARL
                     DGFEFAGELSLGGDLRPVRGALAMALGLQRSRARTGRALVLPQASAREAALVEGLAIH
                     GAAHLLDVVGAMRVAADTAPAALPLAVAEARAGTPALPDMRDVKGQAGAKRALEIAAT
                     GGHSVLMLGSPGTGKSMLAQRFAGLLPPLSQADALESAAVLSLTGAFAHARWGQRVLR
                     APHHTASSVALVGGGSPPRPGEISLAHHGVLFLDEFPEFARAALEALREPLETGRIVI
                     SRAARQAEFPARFQLIAAMNPCPCGHLGSALRACRCTPDAVARYQGRISGPL"
BASE COUNT          367 a          839 c          867 g          432 t
ORIGIN      
        1 cgatcacgcg gtcgacgatc gcgtcgtcca ccgcggcttc gatcttcacc ttgggcagga
       61 aatcgacgac gtactccgca ccgcggtaca actcggtgtg gcccttttgg cggccgaagc
      121 ccttgacttc ggtcaccgtg atgccctgca cgccgatggc actgagggct tcgcggactt
      181 cgtcaagctt gaacggcttg acgatggcgg tcaccatctt catggtcgaa cctctttctg
      241 gtctggggtt gatgcacaac ggcggccggg cgcgatgcgc cgcgaccgcc aaccgggatt
      301 acatggtctt ggtcagggtc acgatgaagc gcgccttgtt cacgctttgg ccatcggacg
      361 agtacttgaa cgactccttc ttgctcgcac cgaccaccgc gcccgcaagg gtcacgccgg
      421 cgccggcgaa cttgctcatg tcgtaggtga cgccgagctt gtagtcgtag tagttgggga
      481 tcgtgccgcc gttggtgtcg gggatgttgt cgcgaatgtc gctggagaac cgggtgtagc
      541 ccacgtgcgc gttgatcgtc agcacatcca tcacggggaa gttgcccgac aggtccaggt
      601 agcccgtgcc gcggcccttg aagccgctgc cgaaggcggc gcctgcgaag ccgaagtagt
      661 cctcggaaac ggtgtgcgag tacttgagcg aggccggccc gtagctgacc gagccgtaga
      721 tctcggtggt gttggcgaag ccgctgccga tgtactggta acgcagcacg ccgacgtcgt
      781 agccgacgtc cttgacgatt tcgccgcggt atccgccgta gaagtccatc tcgatgctgg
      841 agccgccgag cgaatcggga atccagccga tgctcgagtt ccagttgccg acgtagaagc
      901 cgttcatcgt ccagtcgaag ccgccctgga ccgccggacg catcgctttc gtcggggtca
      961 acgagccctg gtcctggccg cggtacttgt agttcgtggt cagactgatg ttggcggtga
     1021 agggcggcgc aggcggcggg tcttccgcat aagcggcggt gccgagcgtc gaagtgatcg
     1081 caagaaggag aatcgacttg ttcatcgtgt ggggctccat tgagggggct tttgagggga
     1141 cttcccccgt tttgcacgat gtgtgccagc gcacttgcac gccgttacgg cacgcgggac
     1201 cgaattcccg agcgacggaa tgggtatttc cgcaccattt agatgcatac gatcaatacg
     1261 caccgattga gtgcgctttg acgcgtactt ctgtgccggc acaatgccgt gcggcgctcg
     1321 ccgcatcgat cttcacgggc tcgaaccatg tcgcttgccg tcatccacag ccgcgcactg
     1381 catgcgctcg aggcggccga ggtcacggtc gaagtccatc tgagcaacgg tttgccgagc
     1441 ttcacgctgg tcggtctggc ggacaccgag gtgaaggagg cacgcgaacg cgtgcgcgcg
     1501 gcgatccaga acagcggcct ggagttcccg cacaacaagc gcatcacggt gaacctcgcg
     1561 cccgccgacc tgccgaagga gtcgggccgc ttcgacctgc cgatcgcgct cggcatcctg
     1621 gccgcgagcg gccagatcga cgcggcgcgg ctcgatggtt tcgagttcgc cggcgaactc
     1681 tcgctgggcg gggacctgcg cccggtgcgt ggcgcgctgg cgatggcgct cgggctgcag
     1741 cgcagccgcg cgcgcaccgg gcgggcactg gtgctgccgc aggcgagcgc gcgcgaggcc
     1801 gcgctcgtcg aagggctggc catccatggc gccgcgcacc tgctcgatgt cgtcggtgcg
     1861 atgcgcgtgg ccgcagacac cgcgcccgcg gccttgccgc tcgccgtcgc cgaagcccgc
     1921 gcgggcacgc cggcgctgcc cgacatgcgc gacgtcaagg gccaggccgg ggcgaagcgc
     1981 gcgctcgaga ttgccgccac cggcggccac agcgtgctga tgctgggctc gcccggcacc
     2041 ggcaagtcga tgctggcgca gcgcttcgcc ggccttctgc cgccgctgtc gcaggccgac
     2101 gcgctcgaat ccgccgcggt gctgagcctg accggcgcgt tcgcgcacgc gcgctggggc
     2161 cagcgcgtgc tgcgcgcgcc gcaccacacc gcgtcatcgg tggcgctggt cggcggcggg
     2221 tcgccgccgc ggcccggcga gatctcgctc gcccaccacg gcgtgctgtt cctcgacgaa
     2281 ttccccgagt tcgcgcgcgc cgcgctcgag gcgctgcgcg agccgctcga gaccggccgc
     2341 atcgtgatct cgcgcgcggc gcggcaggcc gagttcccgg cgcgctttca gctcatcgcg
     2401 gcgatgaatc cgtgcccgtg cggccacctc ggcagcgcgc tgcgcgcctg ccgctgcacg
     2461 ccggatgcgg tggcgcgcta ccagggccgc atcagcggcc cgctg
//
LOCUS       DATMJB010000048         3702 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_703097, whole genome shotgun sequence.
ACCESSION   DATMJB010000048 DATMJB010000000
VERSION     DATMJB010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703097"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VNU48_01135"
     CDS             complement(<1..214)
                     /locus_tag="VNU48_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HWI09913.1"
                     /translation="MIKKFIDKLLGKTPKAGANDSGAAPAGKRVEVPKSEHQIDPKLV
                     DERAVKVVSTLAEAGFEAYVVGGAVRD"
     gene            complement(239..1027)
                     /locus_tag="VNU48_01140"
     CDS             complement(239..1027)
                     /locus_tag="VNU48_01140"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWI09914.1"
                     /translation="MTAAAGRAPGRQGPRSGPLRGPRPARIPSEDRPTYLSGEGPSLT
                     LFDLDDTLLPIDSDHAWGEFMTRLGWVDAAEFARRNDAFYAQYKAGALDIHEYIAFAT
                     EPLRTRTAAQAAAAQRRFMAEVIGPQLRPRALELVQAHRARGDLIALVTATNDFITAP
                     IAQAFGIADLIAVRLERGAGGTITGRIAGTPSYRDGKVTRVQEWLAQQGLGWERLGRI
                     SVYSDSVNDLPLLERATDPVATNPSPALRQVAQARGWRILDLFD"
     gene            complement(1024..1695)
                     /gene="hda"
                     /locus_tag="VNU48_01145"
     CDS             complement(1024..1695)
                     /gene="hda"
                     /locus_tag="VNU48_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HWI09915.1"
                     /translation="MRQIPLAIGVEAARTFDNFVAGANALAIAHLRALAPQAAPVYLW
                     GPSGAGKTHLLHALVGRAEAQGLRAGWFSAADAAPWSFDEAWALIAIDDCDALDVDQQ
                     HAAFALFVEAATRGIPIAGAGRVPPVDLAVREDLRTRLGWGHVFAIAPLGEPEVRATL
                     RREADRRGIFLSDEVMDYLLTRFARDLKHLMAQLDRLDEFSLASKRAITVPLLKQMLV
                     EEGGA"
     gene            complement(1775..2836)
                     /locus_tag="VNU48_01150"
     CDS             complement(1775..2836)
                     /locus_tag="VNU48_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWI09916.1"
                     /translation="MTLTPAQRHTVIWIALSLLAAAIVWLLGPVLTPFVVGAVLAYVL
                     HPLVERLARRRVPRTLAVTVVEVVAIVAVLAVLLLIVPILSRQLPLLREQIPLLADRM
                     NTQLSPWLSHIGIDVALDTASVKAFALKYLDANFDDWFATALSSARIGGSILLAIVGN
                     LVLVPMVLFYLLLDWNELVQRANAMIPPRLREPTHRFFDECDATLGQYLRGQLLVMLS
                     LAVYYSVGLTLFGFDLAVPVGVFTGLAVFIPYVGFGLGLTLALISAVLQFTGWHGVIG
                     VAVVYGIGQLLEGFVLVPRLVGERIGMSALTVIFALLAFGHLFGFVGVLIALPVSAVL
                     VVAVRRLREAYVESELFGG"
     gene            complement(2916..>3702)
                     /locus_tag="VNU48_01155"
     CDS             complement(2916..>3702)
                     /locus_tag="VNU48_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI09917.1"
                     /translation="VSIAQDDYPQLDTQAVLAEIDTLAARLKQRFPADAVPVQRLRWL
                     NRFFFQELGFAGNVNHYYDPDNSYLHRVLDTRRGIPITLAILYIELASQIGLTARGVS
                     FPGHFLIKLRMNTGQQRGEVVIDPFTGHSLSRDELDELLAPYKRNRGLQGDFDVPLGL
                     FLQAATPREVVARMLRNLKEIHRSAHDWRRLLQVQERLVVLLPQAWDERRDRGLVYAD
                     LGIAAAAVRDLSDYLDHTADAPDRVQISARLAELGGGGPLRLH"
BASE COUNT          566 a         1298 c         1337 g          501 t
ORIGIN      
        1 cgtcgcgcac cgcgccgccg acgacgtagg cctcgaagcc ggcctcggcc agggtgctga
       61 cgaccttgac ggcgcgctcg tcgacgagct tcgggtcgat ctgatgctcg ctcttcggca
      121 cctcgacgcg cttgccggcg ggcgcggcgc cggagtcgtt cgcgccggcc ttgggcgtct
      181 tgccgagcag cttgtcgatg aatttcttga tcattcgcga ggcttcaatc gttgcggttc
      241 agtcgaacag gtccaggatg cgccagccgc gtgcctgcgc gacctgccgc agcgccgggc
      301 tcgggttggt ggccaccgga tcggtcgcgc gctccagcag cggcaggtcg ttcaccgagt
      361 cgctgtagac gctgatgcgg cccagccgct cccagcccag cccctgctgc gccagccatt
      421 cctgcacccg cgtgaccttg ccatcgcggt agctgggcgt gccggcgata cggcccgtga
      481 tggtaccccc ggcgccgcgc tcgagccgca ccgcgatcag atcggcgatg ccgaaggcct
      541 gcgcgatagg cgcggtgatg aagtcgttgg tggccgtgac gagcgcgatc aggtcgccgc
      601 gcgcgcggtg cgcctgcacc agctccagcg ccctcggccg cagctgcggg ccgatgacct
      661 cggccatgaa gcggcgctgc gccgcggcgg cctgcgccgc cgtgcgcgtg cgcagcggct
      721 cggtggcaaa ggcgatgtat tcgtggatgt cgagcgcgcc cgccttgtac tgcgcgtaga
      781 acgcgtcgtt gcggcgcgcg aactcggccg cgtcgaccca gcccaggcgt gtcatgaact
      841 cgccccacgc gtgatcggag tcgatcggca gcagcgtgtc gtcgagatcg aacagggtga
      901 gcgacggccc ctcgcccgac aggtacgtcg gtcgatcctc cgaggggatg cgggccggcc
      961 gaggtccacg cagtgggcca cttcgtggcc cctggcggcc cggcgctcgg cccgccgccg
     1021 cggtcatgcg ccgccctcct cgaccagcat ctgcttgagc aacggcaccg tgatcgcgcg
     1081 cttgctcgcg agcgagaact cgtcgagccg gtcgagctgg gccatcaggt gcttgaggtc
     1141 gcgcgcgaag cgcgtcagca ggtagtccat cacctcgtcg gacaggaaga tgccgcgccg
     1201 atcggcctcg cggcgcagcg tggcgcgcac ctccggctcg ccgagcggcg cgatcgcgaa
     1261 cacgtggccc cagcccaggc gggtgcgcag gtcctcgcgc acggccaggt cgaccggcgg
     1321 cacgcgcccg gcgccggcga tcgggatgcc gcgcgtcgcg gcctcgacga agagcgcgaa
     1381 cgccgcgtgc tgctggtcga cgtcgagcgc gtcgcaatcg tcgatcgcga tcagcgccca
     1441 ggcctcgtcg aatgaccagg gtgcggcatc ggcggcgctg aaccagccgg cgcgcaggcc
     1501 ttgcgcctcg gcgcgcccga ccagcgcgtg cagcagatgc gtcttgccgg cgccgctcgg
     1561 accccacaga tagaccggcg cggcctgcgg cgcgagggct cgcaggtgcg cgatcgccag
     1621 cgcattcgcg ccggccacga agttgtcgaa cgtgcgcgcc gcctcgacgc cgatggccag
     1681 cgggatctgc ctcacgggtg cccctcgtcc aatgggtaca tcggccggcc ccccgagggg
     1741 gcgagccttg cttggggcgg cccggcgcgg cggctcatcc gccgaacagc tcgctttcga
     1801 cgtaagcctc gcgcagccgc cgcaccgcga ccacgagcac cgcgctgacc ggcagcgcga
     1861 tcagcacgcc gacgaatccg aacaggtggc cgaacgcgag caacgcgaag atcaccgtca
     1921 acgcgctcat gccgatgcgc tcgccgacga gccgcggcac cagcacgaag ccctcgagca
     1981 gctggccgat gccgtacacc acggccacgc cgatcacgcc gtgccagccg gtgaactgca
     2041 gcacggcgga aatcagcgcc agggtcagcc ccaggccgaa gccgacgtac gggatgaaca
     2101 ccgccagccc ggtgaacacg cccaccggca ccgcgaggtc gaagccgaac agcgtcagcc
     2161 cgacgctgta gtagacggcg agcgagagca tcaccagcag ctgaccgcgc aggtactggc
     2221 cgagcgtggc gtcgcactcg tcgaagaagc ggtgggtagg ctcgcgcagc cgcggcggga
     2281 tcatcgcgtt ggcgcgctgc accagctcgt tccagtcgag cagcaggtag aacagcacca
     2341 tcggcaccag caccaggttg ccgacgatcg ccagcaggat gctgccgccg atgcgcgcgg
     2401 agctcagcgc ggtggcgaac cagtcgtcga agttcgcgtc gaggtacttc agcgcgaagg
     2461 ccttgacgct cgcggtgtcg agcgcgacgt cgatgccgat gtgcgacagc cagggcgaga
     2521 gctgggtgtt catgcggtcc gcgagcagcg ggatctgttc gcgcagcagc ggcagctgcc
     2581 gcgacaggat cggcacgatc agcagcagca cggcgagcac cgcaacgatc gccacgacct
     2641 cgacgaccgt caccgcgagc gtgcgtggca cgcgccgccg tgcgagccgc tcgaccagcg
     2701 ggtgcagcac ataggccagc accgcgccga cgacgaacgg ggtcagcacc ggcccgagca
     2761 gccacacgat cgccgcggcg agcaggctca gggcgatcca gatgacggta tggcgttgcg
     2821 cgggggtgag ggtcatggtg atggctggcg cggcagcgcg cccaccatgt caaggtggcg
     2881 gaattctacg gggcccggcg tacggccccg cgcgatcagt gcagccgcag cggcccgccg
     2941 ccgccgagct cggccaggcg cgcgctgatc tgcacgcggt ccggtgcgtc ggcggtgtgg
     3001 tcgaggtagt ccgacagatc gcgcaccgcg gcggcggcaa tgccgaggtc ggcgtacacg
     3061 aggccgcggt cgcgccgttc gtcccaggcc tgcggcagca gcacgaccag gcgctcctgc
     3121 acctgcagca agcggcgcca gtcgtgcgcg ctgcggtgga tctccttcag gttgcgcagc
     3181 atgcgcgcga ccacctcgcg cggcgtcgcg gcctgcagga acaggcccag cggcacgtcg
     3241 aaatcgccct gcaggccgcg gttgcgcttg tagggcgcga gcagctcgtc gagctcgtcg
     3301 cgcgacagcg agtggccggt gaacgggtcg atcacgacct cgccgcgctg ctggcccgtg
     3361 ttcatgcgca gcttgatcag gaagtgcccg gggaacgaga cgccgcgcgc cgtcaggccg
     3421 atctgcgacg cgagctcgat gtacaggatc gccagcgtga tcgggatgcc gcggcgcgtg
     3481 tcgagcacgc ggtgcaggta gctgttgtcg gggtcgtagt agtggttgac gttgccggcg
     3541 aagccgagtt cctgaaagaa gaaccggttc agccagcgca gccgctgcac cggcacggcg
     3601 tcggccggga agcgctgctt caggcgcgcc gcgagcgtgt cgatttcggc cagcacggcc
     3661 tgcgtgtcga gctgcgggta gtcgtcttgc gcgatcgaca cg
//
LOCUS       DATMJB010000049         6700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_51894, whole genome shotgun sequence.
ACCESSION   DATMJB010000049 DATMJB010000000
VERSION     DATMJB010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51894"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..812
                     /locus_tag="VNU48_01160"
     CDS             126..812
                     /locus_tag="VNU48_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWI09918.1"
                     /translation="MATLLPEISRPGSADAGAPPLRESALYEQVAERLRRRILAHTLH
                     PGSWIDEQALATEYGISRTPLREALKVLAAEGLVTMKLRRGAYVTEVSERDLAEVYHL
                     LALLESDAARVVAERASAGQIAELLALHQQLEAEVGDRDRFFAANERFHMTLLAIADN
                     RWRNQLVADLRKVMKLNRHHSLFKQGRLEASLKEHRQIMAALKARNGERAQLLMQRHF
                     ANGKEAASLS"
     gene            complement(830..2896)
                     /locus_tag="VNU48_01165"
     CDS             complement(830..2896)
                     /locus_tag="VNU48_01165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HWI09919.1"
                     /translation="MSSEPARRRTPSSYYPGIEGLRAVAVLAVLLYHLHAPWVPAGFI
                     GVDIFFVISGFVVSLSVAHFETTSLAGFIARFYDRRLKRIAPALLVCLAAVALLSVLF
                     IPRAVLSESNAKTGLAAFFGLSNVVLARIDGNYFSARVDFNPFTHTWSLGVEEQFYLV
                     APLLLYFWVRAAPGTAVRRRAGAALAILGLASLAWCAWSSSRAPTQAFYGLPSRFWEL
                     ACGVGLFFTLDVWKERLRALPAWMAEAGTLVFACGVGLGLWITRSTSFPFPGVLLPAL
                     ATTGLIAWLVASDGTLVARALSLPVMRYFGRISYSLYLWHWPVFVLFRWTVDLEQPVY
                     RLCAATLAVSLAALSYRFVEAPVRTLSWARLLPTGRMALAGAASLALACTASWAVFKS
                     ADSLSLSVTRDASIWRPDGRIRFDDPERCPLRVMWTPLAAGGVTTYRPKDCVLQAAGR
                     LFVVGDSHAGAYSGMLLGYAARTGHETRLMTLGGCPFVGLRRPDGEGPPECRAFSKAV
                     LQILLRDAQPGDVLFLPSLRVNRLKNQWGAVEDVRDVDNPASRGRAIAEGQRLLAPLA
                     QRRVTILFEAPKPVFLAPPFRCSDWFNRANPICAAGPTMPRSDEEKLRRGTLEAMTEL
                     VSRLPGAGIWDPLPVLCPDSTCDSWRDGKPLYFDGNHLSGFGNAALLPSFTLRMNELW
                     VVSAAS"
     gene            3343..3849
                     /locus_tag="VNU48_01170"
     CDS             3343..3849
                     /locus_tag="VNU48_01170"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HWI09920.1"
                     /translation="MSRPALSLIAAVARNGAIGRDNALLWREAEDLKHFRRVTLGCPV
                     IMGRKTWDSLPPRFRPLPGRRNVVITRDASWRAEGVEAVDSLERALALLDGSAKAFVI
                     GGAEIYALALPHADELVLTEIDAELPGDKFFPAWDRARFRAVAREPHVDANGIGYSFV
                     TYKNSAGD"
     gene            3851..4426
                     /locus_tag="VNU48_01175"
     CDS             3851..4426
                     /locus_tag="VNU48_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HWI09921.1"
                     /translation="MSEGGFHAHGPHDHELEHAAQHDPKGLAGQLAVATAIIATVGAM
                     FSYMGGLTQADAGIYKNNAAIKKTEAANQWAYYQAKSNKQNLAELALDLAPAERRPAY
                     AEQVKRYQGEKATIQNDAKKLEDESKDWDRRSEEQMHQHHRWAQATTALQIAIAMAAI
                     ALLTRRKWLEYGVFALSGLGAVLGALAWQHI"
     gene            complement(4479..5069)
                     /locus_tag="VNU48_01180"
     CDS             complement(4479..5069)
                     /locus_tag="VNU48_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWI09922.1"
                     /translation="MDHVAALPTFRLGSGTAIAQAQPKTTSAVTLDSPALEVMTDLTQ
                     VKAATINPATTLRQAEQMMVYQGVRMLFVVTDMPSIEGLVTATDLKGEAQMRAVHERH
                     VRYDDLCVADVMTGLALLEAVDFERMRAATVGDVVATFKRFGRDHLLVVQNATDQTPR
                     RVRGVISRTQIERQLGSPIDVTPIAISFSEIERALF"
     gene            complement(5189..6700)
                     /locus_tag="VNU48_01185"
     CDS             complement(5189..6700)
                     /locus_tag="VNU48_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI09923.1"
                     /translation="MVAAVMGSSMAFIDGTVVNVALPAIQRDLHASVFEAQWVVEAYA
                     LFLAALLLVGGSLGDRFGRRRLFTLGVALFGLASLGCAAARDVQQLIAARAVQGVGAA
                     LLVPGSLALISAAFPEGERGRAIGTWSGFSGITAAFGPVLGGYLVDRFSWPWAFLINL
                     PFAIAVLIVAWARVPESRNPHAPATLDIRGAVLATLGLGGVVYAFIEAPSRHWSAPAV
                     LLALSVGIAALVGFVVTESRSAAPMLPPHYFRNRNFAGANLLTLGLYAALGGGLFFLP
                     LNLIQVQGYSATAAGAALLPFILIMFALSRWAGRLVDRLGPRPPLVTGPLVAAAGFAA
                     FALPTTGGTYWISFFPAIVVLGFGMTITVAPLTTTVMNAVGPEAAGTASGVNNAVSRV
                     AALLAIAIFGSVMAWAFDHRMDEAMRLVGLPDALTALVHSQRSKLAAIELPAGIDAAT
                     AATVHRAVGDAFISGFRWVMGLSAALALASSLAAALLISGGPRPKAGVGSARE"
BASE COUNT         1222 a         2365 c         2278 g          835 t
ORIGIN      
        1 gtcatccgac atggtgtgtt cactcctgcg ccgcgccccg ccgaatatga ccacatcggg
       61 gctggacggc agcgcacacg gcgcacataa ttcaaaattg aattctatcg tgtcgatcgg
      121 cgcgcatggc gactcttctc cccgaaattt ccaggcccgg cagcgcggac gccggcgcgc
      181 cgccgctgcg cgagagcgca ctctacgagc aggtcgccga gcggctgcga cggcgcatcc
      241 tggcgcacac gctgcacccc ggcagctgga tcgacgagca ggccctggcc accgagtacg
      301 gcatcagccg cacgccgctg cgcgaggcgc tgaaggtgct cgcggccgag ggcctggtga
      361 cgatgaagct gcgccgcggc gcctacgtca ccgaggtgtc ggagcgcgac ctcgccgagg
      421 tctaccacct gctcgcgctg ctcgagagcg acgccgcgcg cgtcgtggcc gaacgcgcca
      481 gcgccgggca gatcgccgag ctgctggcgc tgcaccagca gctcgaggcc gaggtcggcg
      541 accgcgaccg cttcttcgcc gcgaacgagc gcttccacat gacgctgctg gcgattgccg
      601 acaaccgctg gcgcaaccag ctcgtcgccg acctgcgcaa ggtgatgaag ctgaaccgcc
      661 accactcgct gttcaagcag ggccggctcg aggcctcgct gaaggagcac cgccagatca
      721 tggcggcgct gaaggcgcgc aacggcgagc gcgcgcagct gctgatgcag cggcacttcg
      781 cgaacggcaa ggaagcggcc tcgctgagct gaccgcgagc ggccgttgct caggaagcgg
      841 cgctgaccac ccagagttca ttcatgcgca gcgtgaagga cggcagcagc gcagcgttgc
      901 cgaagccgct caggtggttg ccgtcgaagt acagcggctt gccgtcgcgc cagctgtcgc
      961 aggtgctgtc ggggcacaga accggcagcg ggtcccagat gcccgcgccc gggagccgcg
     1021 agaccagttc ggtcatcgcc tcgagcgtgc cgcggcgcag cttttcctcg tcgctgcgcg
     1081 gcattgtcgg tccggccgcg cagatcggat tggcccggtt gaaccaatcg gagcaccgga
     1141 acggcggcgc cagaaacacc ggcttcggcg cctcgaacag gatcgtcacg cgccgctgcg
     1201 caaggggcgc caacagccgc tgcccctcgg cgatcgcgcg cccccgcgac gccgggttgt
     1261 cgacatcgcg tacgtcctcg acggcgcccc actggttctt cagccgattg acgcgcagcg
     1321 aaggcaggaa cagcacgtca ccgggttgcg cgtcccgcaa caggatctgc agcaccgctt
     1381 tcgaaaacgc cctgcactct ggcgggccct cgccgtccgg gcgacgcagg ccgacgaacg
     1441 ggcaaccacc caatgtcatc aggcgggtct cgtggccggt tctcgcggca tagccgagca
     1501 gcatgccgga atacgccccg gcgtgcgaat cgccgacaac gaacaggcgc cccgcggcct
     1561 gcagcacgca gtccttcggc cggtaagtgg tgacgccgcc cgcggcgagg ggagtccaca
     1621 tgacccggag cgggcagcgc tccgggtcat cgaagcggat gcggccgtcg gggcgccaaa
     1681 tcgacgcgtc gcgcgtgacg ctcagcgaca gcgaatcggc ggacttgaag accgcccacg
     1741 acgcggtgca cgcgagcgcc agcgacgccg cgccggcgag cgccatgcga ccggtcggca
     1801 acagtcgcgc ccatgacaga gtgcgcaccg gcgcctcgac gaagcggtac gagagcgcgg
     1861 cgagcgatac cgccagcgtc gctgcgcaaa ggcgatagac cggctgctcc aagtccaccg
     1921 tccagcgaaa cagaacgaag accggccagt gccacaggta cagcgagtac gagatccgcc
     1981 cgaaatagcg catcaccggc agcgacaacg cgcgcgcgac caacgtgccg tctgaggcga
     2041 ccagccacgc gatcagcccg gtcgtcgcga gtgccgggag cagcacgccg ggaaacggga
     2101 aggaggtgct gcgcgtgatc cacaggccca acccgacacc acatgcgaac accagcgtcc
     2161 ccgcctcggc catccacgcc ggtagagccc gcagccgctc cttccacacg tcgagcgtga
     2221 agaacaggcc cacgccgcag gccaattccc agaagcgcga aggcaggccg tagaacgcct
     2281 gcgtcggcgc ccgcgacgac gaccaggcgc accaggccag cgaggccagg cccaggatcg
     2341 ccaaggcggc gccggcgcgc cggcgcaccg ccgttccggg cgccgcccgg acccagaagt
     2401 agagcagcag cggcgcgacc agataaaact gttcctcgac cccgagcgac cacgtgtgcg
     2461 taaaggggtt gaagtcgacg cgcgccgaga agtagttgcc gtcgattcgg gccagcacga
     2521 cattgctcag gccgaagaat gccgccagcc cggtcttcgc attcgattcg ctgagcaccg
     2581 cgcgcgggat gaacagcacc gacagcagcg ccaccgccgc cagacacacg agcagcgccg
     2641 gcgcgatgcg cttcagccgc cggtcgtaga agcgggcgat gaagccggcc aggctggtgg
     2701 tctcgaaatg cgccaccgac aggcttacca cgaagcccga gatcacgaag aagatgtcga
     2761 cgccaatgaa gccggccggc acccagggcg catgcaagtg gtacagcagc acggccagga
     2821 ccgcgaccgc gcgaaggcct tcgatgcccg ggtagtagct cgacggcgtg cggcggcggg
     2881 ccggttcgga tgacatggac gttgacgcca gttgcattct gtcgacggcg tgttgggacc
     2941 ctgcgcggtg gcgttgcgat gatgccgaag ctttcgcgcc gcttggcggc gaaatcgaaa
     3001 ctatcggaaa gcggttcggc gaagcatcgc tggggatgca gcagtcgccg acccggccgc
     3061 ttgcgacgac cgtcggtgcg cggacccatc gagacgccct gtcgccgaaa ggcgcaacgc
     3121 tctcccgtga aggtcgtgcc cggcacattt gatgacccta cgacggcgtt cccgatgcca
     3181 aggatcgcgg cgacctgatc gcctacctga agcaggtgaa cgacaccccc cgaacgcgcg
     3241 actaaccgct gacacaagtg gcgcccgccg tgcacaccgg cgcccgcgcc cacgccgagc
     3301 cgagccgaag ccggttgtgc gaggctgcga caatcgcgcc ccatgagccg ccccgccctg
     3361 agcctgatcg ccgccgtcgc gcgcaacggc gcgatcggcc gcgacaacgc gctgctgtgg
     3421 cgcgaggccg aagacctgaa acacttccgg cgcgtcacgc tgggctgccc ggtgatcatg
     3481 ggccgcaaga catgggactc gctgccgccg cgcttccggc ccctgccggg ccggcgcaat
     3541 gtggtgatca cgcgcgatgc gtcctggcgc gccgaaggcg tcgaggccgt cgactcgctc
     3601 gagcgcgcgc tggcgctgct cgacggcagc gcgaaggcct tcgtgatcgg cggcgccgag
     3661 atctacgcgc tcgcgctgcc gcacgccgac gaactggtgc tgaccgagat cgacgccgag
     3721 ctgcccggcg acaagttctt cccggcctgg gatcgtgccc gcttccgcgc cgtcgcgcgc
     3781 gagccgcacg tcgacgcgaa cggcatcggc tacagcttcg tcacttacaa gaactctgca
     3841 ggagattgac atgtcagaag gtggattcca cgcgcacggc ccgcacgatc acgagctcga
     3901 gcatgcggcg cagcacgatc cgaaggggct ggcgggccag ctcgcggtgg ccaccgcgat
     3961 catcgcgacg gtcggggcga tgttctcgta catgggcggt ttgactcagg ccgacgccgg
     4021 catctacaag aacaatgccg cgatcaagaa gaccgaggcc gcgaaccagt gggcctacta
     4081 ccaggccaag agcaacaagc agaacctggc cgagctcgcg ctcgacctcg cgccggccga
     4141 gcgccggccc gcgtatgccg agcaggtcaa gcgctaccag ggcgagaagg ccacgatcca
     4201 gaacgacgcg aagaagctcg aggacgagtc gaaggactgg gaccgccgca gcgaagagca
     4261 gatgcaccag catcaccgct gggcgcaggc gacgaccgcg ctgcagatcg cgatcgcgat
     4321 ggcggcgatc gcgctgctga cgcgccgcaa gtggctcgag tacggcgtgt tcgcactcag
     4381 cgggctgggc gccgtgctgg gcgctctggc ctggcagcac atctgaaccg aaggagcggt
     4441 cgggtcgacg acacggagcg cagcaccgtt tcagcccctc agaacagcgc ccgctcgatc
     4501 tcggaaaagc tgatggcgat cggcgtgacg tcgatcgggc tgccgagttg ccgctcgatc
     4561 tgcgtccggg aaatcacacc ccgcacgcgg cgcggcgtct ggtcggtggc gttctgaacg
     4621 accagcaggt ggtcccggcc gaagcgcttg aacgtcgcga ccacgtcgcc gacggtggcg
     4681 gctctcatgc gttcgaagtc gacggcctcg agcagcgcca atcccgtcat cacatcggcg
     4741 acgcacaggt cgtcgtagcg gacatggcgc tcgtgaacgg cgcgcatctg ggcctcgccc
     4801 ttcaggtcgg tggcggtgac caggccttcg atggacggca tgtccgtcac gacgaacaac
     4861 atgcgcacgc cttgatagac catcatctgc tcggcctggc gcaaggtggt ggcggggttg
     4921 atggtcgcgg ccttgacctg cgtcaggtcc gtcatcacct cgagcgccgg cgagtcgagc
     4981 gtgaccgccg aggtggtctt cggttgggcc tgggcgatgg ccgttcccga tccaaggcga
     5041 aacgtcggca gcgccgcaac gtgatccatg ctcggcctcc catatcacga cagatgccgg
     5101 cgaggttatc ccgatgcaaa tcggctgtcc agacgccctt ccgcggcgct gcgacggccg
     5161 cggccgcacg tgacccggtc ggccccgatc actcgcgcgc actgccgacg ccggccttcg
     5221 gccgcggacc gcccgaaatc agcagcgccg cggccagcga actggccagc gccagcgccg
     5281 cgctgagccc catcacccag cgaaaccccg aaatgaacgc gtcacccacc gcgcgatgca
     5341 ccgtcgccgc ggtcgcggcg tcgatgccgg cgggcaactc gatggcggcg agtttgctgc
     5401 gctgcgaatg caccagcgcg gtcagcgcgt cgggcagtcc gaccagccgc atcgcttcat
     5461 ccatccggtg atcgaatgcc caggccatca cgctgccgaa gatcgcgatc gccagcagcg
     5521 ccgccacccg cgacaccgcg ttgttgacgc ccgacgcggt gccggcggcc tcggggccga
     5581 cggcgttcat gacggtcgtg gtgagcggcg caacggtgat cgtcatgccg aagccgagca
     5641 cgacgatggc aggaaagaag ctgatccagt aggtgccgcc ggtcgtgggc aacgcgaagg
     5701 cggcgaatcc cgccgcggcg acgagcggcc ccgtcaccag gggcggccgt ggtcccaggc
     5761 ggtccacgag ccgcccggcc cagcgcgaca acgcgaacat gatcaggatg aacggcagca
     5821 acgccgcgcc ggccgcggtc gccgagtagc cctgcacctg gatcaggttc agcggcagga
     5881 agaacaggcc accgccgagc gcggcataca gccccagggt cagcagattg gcgccggcaa
     5941 agtttcgatt gcggaagtag tgcggcggca gcatcggcgc ggcgcttcgc gactcggtca
     6001 cgacgaagcc caccagcgcc gcgatgccca ccgacagcgc cagcagcacc gccggcgcgc
     6061 tccagtgccg cgacggcgcc tcgatgaagg cgtagaccac gccaccgagg ccgagcgtgg
     6121 ccagcaccgc gccgcgaata tcgagtgtcg ccggggcgtg cgggttgcga ctctcgggca
     6181 cgcgggccca ggcgacgatc agcacggcga tcgcgaacgg caggttgatc aggaatgccc
     6241 agggccagga gaagcggtcg accaggtagc cgccaagcac cggcccgaag gccgcggtga
     6301 tgccgctgaa gcccgaccag gtgccgatcg cacgaccgcg ctctccttcg ggaaacgccg
     6361 cgctgatcaa tgccaggctg cccggcacga gcagcgcagc gcccacgccc tgcacggcgc
     6421 gggcagcgat caactgctgc acatcgcgcg ccgcggcgca gccgagcgag gccaggccga
     6481 agagcgcgac gccgagcgtg aacagccggc gccgcccgaa gcggtcgccc agcgaccctc
     6541 ccaccagcag cagcgcggcc aggaacagcg catacgcctc gacgacccac tgcgcctcga
     6601 acacgctggc gtgcagatcg cgttgtatcg ccggcagcgc gacgttgacg acggtgccgt
     6661 cgatgaacgc catgctcgac cccatcaccg cggcgaccag
//
LOCUS       DATMJB010000050         3158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_583889, whole genome shotgun sequence.
ACCESSION   DATMJB010000050 DATMJB010000000
VERSION     DATMJB010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583889"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..809
                     /locus_tag="VNU48_01190"
     CDS             90..809
                     /locus_tag="VNU48_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09924.1"
                     /translation="MIFGTHPSESVKAEGELHGPRVLQAVKDALADGPAPGPGQLTAR
                     IRAPLTQLGDAHGADLLAREVAGLLLGFAPTVHGNFLTVMRNWIESGHLWVLQQDFAE
                     RIQAGATPLAAARAVLMRPMLDAMQHEPVPPMVWRRPVVNGAPDPAKPPVVLGLASAI
                     ESLDPVRDQARRDALLFGGDYFADKASGHWGLHACPGSKMGVGVMLAMAAELLDAGTL
                     RPTGSPVLLMLTPKVPVAPTP"
     gene            complement(819..>3158)
                     /locus_tag="VNU48_01195"
     CDS             complement(819..>3158)
                     /locus_tag="VNU48_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWI09925.1"
                     /translation="IEALAEGSASTEARGRPRTPMIGRQAEFARLTQALDTACAGQPA
                     GAEVAGPPGIGKTRLVEEFLHHARQQDCRVLRGNCEANLGGTPLQPFRQMAQSLGVAE
                     PPSGPRAWCEWFERLAAEQPLVLFVDDWQWADDASHQVLAAAQRLAGTRLLTVLTRRV
                     SGAPTAATQDLETIALAPLDEATAARAVAAQLPGADPFVVAQICARAGGNPLFIEELC
                     HSAARGDSGRLAPSSSPAGWLEQLIESRVQHLSPALVDMVRTAAVIGNSVPAWLLARI
                     VGCAADSPLVRELAEQDFLFPGEHVGTLRFKHGLTRDVIYASLGLLGRQVLHLRIGAA
                     LMHHAAAGGEEDAEALALHFGAGGDPALEAHYAERAGDRALAVSALDRARAHYRAALA
                     ALERGELAGESALRWVDIVQRLAMVCMFDPERSQIELAERAVVLAERHGNPLQVARAR
                     HWLGCVNYALGEPRAAVAHGERALLEAEQAGDEPLAVQVVASLGEAHTAAGRYARAAA
                     LLDRAIEVKRSHRTGRRTNVGLAYSLVCRGVLLGDRGSFTSAAEYFDQALDCVVGLTH
                     QIGASIQGWRGAVLLWQGRWAAASEAATESARIAEATRSLWQLSIARAMDGYAHWMLE
                     RRPQALEGIVAVTDWLLPHDSGLFRSLNHGWLAEGLQSLGRPAEARRQAVLALQRGRH
                     GDRIGLAMACRAMARAAHAAGRPVAAQRQLACAYRVARGRDSAHELAVTQLAEAELAI
                     ATGGAASRARALIDEAATAFDTMAMEWHLAQALQLRERL"
BASE COUNT          422 a         1201 c         1125 g          410 t
ORIGIN      
        1 acgtgcacat ggacgtcgcc ggccggtttc cgatgcccga ccccgcgcga aggccggggt
       61 gtccggggca catcatcggc ccctcgcgca tgatcttcgg cacccatccg tccgagtccg
      121 tgaaggccga aggcgagctc cacggcccgc gcgtgctgca ggctgtcaag gacgcgctgg
      181 ccgacgggcc ggcccccgga ccaggccagc tcaccgcccg catccgcgcc ccattgacgc
      241 agctgggtga tgcccacggc gccgacctgc tggcacgcga ggtcgccggc ctgttgctcg
      301 gcttcgcgcc caccgtgcac ggcaacttcc tcaccgtgat gaggaactgg atcgagagcg
      361 ggcatctctg ggtgctgcaa caggacttcg ccgaacggat ccaggccggt gccacgccgc
      421 tggccgccgc acgcgccgtg ctgatgcggc cgatgctgga tgcgatgcag cacgaacccg
      481 tgccgccgat ggtctggcgc cggcccgtgg tgaatggtgc gcccgacccg gcgaagccgc
      541 cggtcgtgct cgggctggcc tccgcgatcg agagcctcga cccggtccga gaccaggctc
      601 ggcgcgacgc gctgctgttc ggcggcgact actttgcaga caaggcatcg ggccactggg
      661 gcctgcacgc ctgccccggc tcgaagatgg gcgttggcgt gatgctcgcg atggccgccg
      721 agctgctcga tgccggcacg ctgcgcccca ccggctcgcc ggtgctgctg atgctgacac
      781 cgaaggtgcc ggtggcgccg acgccctgac tgaaaacgct aaagccgctc gcgaagctgc
      841 agcgcctggg ccaggtgcca ctccatcgcc atcgtgtcga aggccgttgc cgcttcatcg
      901 atcaacgcgc gcgcgcggga cgctgcgccc cccgtggcga tcgcgagttc cgcctcggcg
      961 agttgggtca cggcgagttc gtgcgctgaa tcgcgcccgc gcgccacgcg ataggcacag
     1021 gccagctggc gctgtgccgc gacgggccgc ccggcagcgt gcgcggcgcg cgccattgca
     1081 cggcaggcca tggccagacc gatgcggtcg ccgtggcggc cgcgctgcag cgccagcacg
     1141 gcctgccgac gcgcctcggc cgggcgcccc agcgactgca ggccttcggc cagccagccg
     1201 tggttcagcg aacggaacag cccgctgtcg tgcggcaaca gccagtcggt gaccgcgacg
     1261 atgccctcca gcgcctgcgg cctgcgctcg agcatccagt gcgcgtagcc gtccatcgcc
     1321 cgcgcgatcg acaactgcca caggctgcgc gttgcctcgg cgatgcgcgc cgactcggtg
     1381 gcggcctcgc tggccgcagc ccagcgcccc tgccacagca gcaccgcgcc gcgccagccc
     1441 tggatgctgg cgccgatctg gtgcgtcagg cccacgacgc agtccagcgc ctggtcgaaa
     1501 tactccgctg ccgaagtgaa gctgccgcgg tcgccgagca gcacgccacg gcacaccagc
     1561 gagtacgcca ggccgacgtt cgtgcgccgg ccggtgcggt ggctgcgctt gacttcgatc
     1621 gcgcggtcca gcagcgcggc cgcgcgtgca tagcggccgg ccgcggtgtg cgcctcgccc
     1681 agcgaggcca ccacctgcac ggccagcggt tcgtcgcccg cctgctcggc ctccagcagc
     1741 gcacgttcac cgtgcgcgac cgccgcacgc ggttcaccca gcgcgtagtt cacgcagccc
     1801 agccagtggc gcgcgcgcgc cacctgcagc gggttgccgt ggcgttcggc cagcaccacc
     1861 gcgcgttcgg ccagctcgat ctggctgcgc tcgggatcga acatgcacac catcgccagg
     1921 cgctgcacga tgtccaccca gcgcagcgcc gattcgccgg ccagctcgcc gcgctcgagc
     1981 gcggccagcg ccgcgcggta gtgcgcgcgt gcgcggtcga gcgccgagac ggccagcgcg
     2041 cggtcgccgg cacgttcggc gtagtgcgcc tccagtgcgg ggtcgccgcc ggcaccgaag
     2101 tgcagcgcga gcgcctcggc gtcttcttcg ccgccggcgg cggcatggtg catcaacgcc
     2161 gcaccgatgc gcaggtgcag cacctggcgc ccgagcaggc cgagcgacgc gtagatcacg
     2221 tcgcgcgtca agccgtgctt gaagcgcagc gtgcccacgt gctcaccggg gaacagaaag
     2281 tcctgttcgg ccagctcgcg caccagcggg ctgtcggcgg cgcagccgac gatgcgggcc
     2341 agcagccagg ccggcaccga attgccgatg acggcggccg tgcgcaccat gtcgacgagt
     2401 gccgggctca ggtgctgcac gcgcgactcg atcagctgct cgagccaacc cgcggggctg
     2461 gacgacggcg ccaggcgtcc gctgtcgccg cgcgcggccg agtggcacaa ctcctcgatg
     2521 aacagcgggt tgccgccggc gcgcgcgcag atctgggcca ccacgaacgg atcggctccg
     2581 ggcagctgag ccgccacggc ccgcgcggcc gtggcctcgt ccagcggcgc gagcgcgatc
     2641 gtttcgaggt cctgcgtcgc ggcggtcggc gcgccgctga cgcgccgggt cagcacggtc
     2701 agcaaccgcg tgccggccag gcgctgcgcg gcggccagca cctggtgcga ggcgtcgtcg
     2761 gcccattgcc agtcgtcgac gaagaggacc agcggctgct cggcagccag gcgttcgaac
     2821 cactcgcacc aggcgcgcgg ccccgacggc ggctcggcca cccccagcga ctgcgccatc
     2881 tggcggaaag gctgcagcgg cgtcccgccg agattggctt cgcagttgcc gcgcagcacg
     2941 cggcagtcct gctggcgcgc atggtgcagg aactcctcga ccagccgcgt cttgccgatg
     3001 ccgggcggcc ccgccacctc cgcgccggcc ggctggccgg cgcacgccgt gtcgagcgcc
     3061 tgcgtgagcc gcgcgaactc cgcctggcgg ccgatcatcg gcgtgcgcgg gcggccgcgg
     3121 gcctcggtgc tggcggaccc ttcggcgagc gcctcgat
//
LOCUS       DATMJB010000051         7329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_406296, whole genome shotgun sequence.
ACCESSION   DATMJB010000051 DATMJB010000000
VERSION     DATMJB010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406296"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VNU48_01200"
     CDS             <1..515
                     /locus_tag="VNU48_01200"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HWI09926.1"
                     /translation="LGALTGVCANAQTQAPGLWEHTMKSQDGQMEAAMAQMRQQMESM
                     PPEQRKKIEEMMASRGVKTGAQGTSAKFCLTKEQAAKGPEPHMSGDCSRHDLTRSGNT
                     VKYSFECSKPRPMKGEGEMTFASDKAYSGRSTVSSQVNGQPRQMSMEMSGKWLSADCG
                     DIKPLAMPAR"
     gene            512..1786
                     /locus_tag="VNU48_01205"
     CDS             512..1786
                     /locus_tag="VNU48_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HWI09927.1"
                     /translation="MTGPASASGPGARVTGRWPVALAGAVSLAVAMGIGRFAFTPLLP
                     MMLHDGVIDLHAASWLATANYFGYLAGALLCTFQPWIWARVRHLPTVNGPWLVRAGLV
                     ATGLLTLGMALPLPASWPLLRFAAGVASALVFVYTSGWCLAQLARRDSAALGGVMYTG
                     PGAGIVVSGLFASGMVQWQWRAATGWAIFAVLAFALTAAVWRIFKGGAPPQPAPGAAA
                     PVGRAQGVGHGRAEIGVLALAYGIAGFGYIVTATFLPVIARAALPGSAWLDMFWPIFG
                     AGVMLGALIATRLHIGGDLRRLLAGCYLVQAAGIGIGVWSPSLAGFAIGSLLLGIPFT
                     AITFFAMQEVRRLRPLLAASFMGLLTAMYGIGQIVGPPLVALLLRRSANAGAGFALAL
                     EIAAGALGFGAGLYLWMMRAYPVQHRMRLADV"
     gene            complement(1783..2139)
                     /locus_tag="VNU48_01210"
     CDS             complement(1783..2139)
                     /locus_tag="VNU48_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HWI09928.1"
                     /translation="MKSGGRGGLVYSTDAGRMCPACRQPIAACVCKQQATPLGDGIVR
                     VSRETKGRAGKGVTLVKGLALDAAALAQTARQLKASCGSGGTVKDGVIEIQGDHVERV
                     IDRLKQDGWTVKRAGA"
     gene            complement(2222..3202)
                     /locus_tag="VNU48_01215"
     CDS             complement(2222..3202)
                     /locus_tag="VNU48_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HWI09929.1"
                     /translation="MLLTRRTITLAAALGWLTGAAHAQGQWPSKPISYIVPFPAGGTT
                     DVLGRLIGQKLGTALGQTIVIDNRGGAGGSLGSEIAARAAPDGYTLLGGTISSHAINV
                     SLYPKLGYDPIKSFTPITLIGTNPVVLVVNQASPYKTLADVVAAAKAKKPLSSASAGS
                     GTSQHLALELLKAKAGIDITHIPYKGSGPAIQDVIGGQVDMMFDTTVVAGPHIEGGKL
                     RALAVTSAKRIASMPNVPTVAESGVAGLADYEVTSWQAIFAPAGTPRPIVDRLHVEIA
                     KILKEPEMQERIAKLGMQGADMSTEQVAAFQKAEVAKWAAVIKGANIKLE"
     gene            3318..4907
                     /gene="garD"
                     /locus_tag="VNU48_01220"
     CDS             3318..4907
                     /gene="garD"
                     /locus_tag="VNU48_01220"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HWI09930.1"
                     /translation="MPETAARAPLCIAMHPLDNVAIVANDGGLPAGTVASNLMLVERV
                     PQGHKVALIDIAAGDAVRRYNVVIGRAARAIAKGSWVHERLLELPAARSLDGLPIATV
                     RPAPMPPLEGHTFEGYRNADGSVGTRNILAITTTVQCVAGVVAFAVGRIKAELLPKYP
                     NVDDVVGLEHSYGCGVAIDAPGAEIPIRTLRNITLNPNFGNEVMVVSLGCEKLQPERL
                     LPPGSFAIVANDDAARDASPELDVVCLQDAGHIGFMSMIVSIMTTAESHLARLNARRR
                     ETCPASALVVGVQCGGSDAFSGVTANPAVGFCTDLLVRAGATVMFSETTEVRDGIAQL
                     TARAATSEVADAMIREMAWYDAYLARGRVDRSANTTPGNKQGGLSNIVEKAMGSIVKS
                     GSAPITGVLSPGERLRDRGITGGLVYAATPASDFICGTLQLAAGINLHVFTTGRGTPY
                     GLAEVPVIKVATRSDLARRWHDLMDVNAGRIADGTASIDEVGWELFRLMLEVASGRRK
                     TWAEHWKLHNALVLFNPAPVT"
     gene            4955..5881
                     /locus_tag="VNU48_01225"
     CDS             4955..5881
                     /locus_tag="VNU48_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI09931.1"
                     /translation="MPPIISVADVSKTYASGFRALDHVSLEVQPGEIFALLGPNGAGK
                     TTLIGLICGIVKLGEGSVKVAGLDIVADYRAVRSMIGLVPQELTTDAFETVWATVSFS
                     RGLFGKPVDPAYTEKTLRALSLWDKKDSKIMTLSGGMKRRVMIAKALSHEPRVLFLDE
                     PTAGVDVALRKDMWAMVRSLRESGVTIVLTTHYIEEAEDMADRVGVISHGKIILVEDK
                     VTLMHKLGKKQLVLQLQQPLARLPESLAAHALNLNAQGTELTYVYDPRASRNDVAALL
                     DDLRRTGIGLRDLQTTQSSLEDIFVSLVEGAR"
     gene            5878..6639
                     /locus_tag="VNU48_01230"
     CDS             5878..6639
                     /locus_tag="VNU48_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI09932.1"
                     /translation="MNLHAIRAIYAFEMARTWRTITQSIISPVLSTALYFVVFGSAIG
                     SRIDSIGTVSYGAFIVPGLIMLSVLTQSISNASFGIYFPKFTGTIYELLSAPISHVEI
                     VLSYVGAAATKSVLVGLIILGTAALFVPIHMAHPVWMLLFLVLTAVTFSLFGFIIGIW
                     ADDFEKLQLIPLLIVMPLTFLGGPFYSISMLPPLWQKLSMLNPSVYLISGFRWSFFGV
                     AEMHVGISFSVTLLFFVVALWFATWIMRSGWRLKA"
     gene            6760..7077
                     /locus_tag="VNU48_01235"
     CDS             6760..7077
                     /locus_tag="VNU48_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09933.1"
                     /translation="MKRLKVILIAAMLGATCGAAFAAEPGSDAERRERMDRAYEDYRN
                     QAPGPAARTESSIKRGAHRAGSAIKHGAQKVGHAVGKGVRKTGEAIGHGGEKLEDKST
                     PKP"
     gene            7112..>7329
                     /locus_tag="VNU48_01240"
     CDS             7112..>7329
                     /locus_tag="VNU48_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09934.1"
                     /translation="MKVFAVSKVRLDADGRITHVCWGPVDTRKNDWAAAETVAPVADA
                     VRAIHDGHKVYALFPSTHGHLPDRPFMVV"
BASE COUNT         1076 a         2556 c         2553 g         1144 t
ORIGIN      
        1 tgctgggtgc gctgaccggt gtctgtgcca acgcgcagac ccaggcgcca ggcctctggg
       61 agcacacgat gaagtcacag gacggtcaga tggaggctgc gatggcgcag atgcgccagc
      121 agatggagtc catgccgccc gagcagcgca agaagatcga agagatgatg gccagccgcg
      181 gcgtcaagac gggtgcgcag ggcacgagcg cgaagttctg cctgaccaag gagcaggcgg
      241 cgaaaggccc cgagccgcac atgagcggcg actgcagccg ccacgacctg acccgcagcg
      301 gcaacacggt caagtacagc ttcgaatgca gcaagccgcg tccgatgaag ggcgagggtg
      361 agatgacctt cgccagcgac aaggcctaca gcggcaggtc caccgtgagc tcgcaggtca
      421 acggccagcc gcggcagatg agcatggaga tgagcggcaa gtggctgtcg gccgattgcg
      481 gcgacatcaa gccgctggcg atgcccgccc ggtgaccggc cccgcttcgg ccagcggtcc
      541 cggcgcgcgt gtcaccggcc gctggccggt cgcgctcgcg ggcgcggtct cgctcgcggt
      601 cgcgatgggc atcggccgct tcgcgttcac gccgctgctg ccgatgatgc tgcacgacgg
      661 cgtgatcgac ctgcacgccg ccagctggct cgccaccgcg aactacttcg gctacctcgc
      721 tggcgcgctg ctgtgcacct tccagccgtg gatctgggcc cgggtgcggc acctgccgac
      781 ggtcaacggg ccctggctgg tgcgcgccgg gctggtcgcg accgggctgc tgaccctggg
      841 catggcgctg ccgctgccgg cgagctggcc gctgctgcgc ttcgccgccg gcgtggcgag
      901 cgcgctggtg ttcgtctaca cctcgggctg gtgcctggcg caactcgcgc ggcgcgactc
      961 ggctgcgctg ggcggcgtga tgtacaccgg gccgggcgcg ggcatcgtcg tcagcggcct
     1021 gttcgccagc ggcatggtgc agtggcagtg gcgggccgcg accggctggg ccatcttcgc
     1081 cgtgctggcg ttcgcgctca ccgccgccgt gtggcgcatc ttcaagggtg gcgcgccacc
     1141 gcagccggcg cccggcgcgg ccgcgccggt ggggcgggcg cagggcgtcg gccatggtcg
     1201 cgccgagatc ggtgtgctgg cgctggccta cggcatcgcc ggcttcggct acatcgtcac
     1261 cgcgaccttc ctgccggtga tcgcgcgcgc cgcgctgccc ggctcggcgt ggctcgacat
     1321 gttctggccg atcttcggcg ccggcgtgat gctcggcgcg ctgatcgcca cgcgcctgca
     1381 catcggcggc gacctgcgcc gcctgctcgc cggctgctac ctcgtgcagg cggcgggcat
     1441 cgggatcggc gtctggagcc cgagccttgc cggcttcgcg atcggcagcc tgctgctggg
     1501 catcccgttc accgcgatca ccttcttcgc gatgcaggag gtgcgccggc tgcgcccgct
     1561 gctcgcggcg agtttcatgg ggttgctgac cgcgatgtac ggcatcggcc agatcgtcgg
     1621 gccgccgctg gtcgcgctgc tgctgcggcg cagcgcgaac gccggcgccg gcttcgcgct
     1681 ggcactcgag atcgcggcgg gcgcgctcgg cttcggtgcc ggcctgtacc tgtggatgat
     1741 gcgggcctat ccggtgcagc accggatgcg cctggccgac gtctaagcgc cggcgcgctt
     1801 gaccgtccag ccgtcctgtt tcagccggtc gatgacgcgc tcgacgtgat cgccctggat
     1861 ctcgatcacg ccgtccttca cggtgccgcc gctgccgcag ctcgccttca gctgcctggc
     1921 cgtctgcgcc aacgccgcgg cgtcgagtgc caggcccttc accagcgtca cgcccttgcc
     1981 ggcgcggccc ttggtctcgc gcgagacgcg cacgatgccg tcgccgagcg gcgtcgcctg
     2041 ctgcttgcac acgcacgcgg cgatcggttg ccggcaggcc gggcacatgc gtccggcgtc
     2101 ggtcgagtag accagcccgc cccggccacc gctcttcatg gcgccgcctt gccttcagcc
     2161 gcgttggctg ccgtggccca tcatgccaag gcgccgatga acccgaggcc ttgcatgcgc
     2221 ctcactccag cttgatgttc gcgcccttga tcaccgcggc ccacttcgcg acctcggcct
     2281 tctggaacgc tgcgacctgc tcggtgctca tgtcggcgcc ctgcatgccg agcttggcga
     2341 tgcgctcctg catctcaggc tccttcagga tcttcgcgat ctcgacgtgc agccggtcga
     2401 cgatgggccg gggcgtgccg gccggcgcga agatcgcctg ccacgaagtc acctcgtagt
     2461 cggccaggcc cgcaacgccg ctctcggcga ccgtcggcac gttcggcatg ctggcaatgc
     2521 gcttcgccga ggtcaccgcg agcgcgcgca gcttgccgcc ctcgatgtgc gggccggcga
     2581 ccacggtggt gtcgaacatc atgtcgacct ggccgccgat cacgtcctgg atcgccgggc
     2641 cgctgccctt gtacgggatg tgcgtgatgt cgatgccggc cttggccttc agcagttcga
     2701 gcgcgaggtg ctgcgaggtg ccgctgccgg ccgacgccga gctcagcggc ttcttcgcct
     2761 tcgcggccgc caccacatcg gccagcgtct tgtacgggct cgcctggttc acgaccagca
     2821 ccaccggatt cgtgccgatc agggtgatcg gcgtgaacga cttgatcggg tcgtagccga
     2881 gcttggggta caggctcacg ttgattgcat gcgagctgat cgtgccgccg agcagcgtgt
     2941 agccgtcggg cgcggcgcgc gcggcgatct cggagcccag gctgccgccg gcgccgccgc
     3001 ggttgtcgat gacgatcgtc tggccgagcg cggtgcccag cttctggccg atcagccggc
     3061 cgagcacgtc ggtcgtgccg ccggccggaa acggcacgat gtaactgatc ggcttgctcg
     3121 gccactggcc ttgcgcgtgc gccgcgccgg tgagccagcc gagcgcggcg gcgagagtga
     3181 tggtgcgacg ggtcagcaac atgcgagagg tctccgagga ttcaaaggta tgacgattgc
     3241 cgcaaagcgc cgccgcgtca atcggtgctg atgcccagcc tggcgcgatc gccagcgatt
     3301 taccggagaa tcgcgccatg cccgagaccg ccgcccgcgc cccgctgtgc atcgcgatgc
     3361 acccgctcga caacgtcgcg atcgtcgcga acgacggcgg cctgccggcc ggcacggtcg
     3421 catcgaacct gatgctggtc gagcgcgtgc cgcagggcca caaggttgcg ctgatcgaca
     3481 tcgcggccgg cgatgcggtg cggcgctaca acgtcgtgat cgggcgcgcc gcgcgcgcga
     3541 tcgcgaaggg cagctgggtc cacgaacgcc tgctcgaact gcccgcggcg cgctcgctcg
     3601 atgggctgcc gatcgcgacc gtgcggcccg cgcccatgcc gccgctcgag gggcacacct
     3661 tcgagggcta ccgcaacgcc gacgggtcgg tcggcacgcg caacatcctc gcgatcacga
     3721 cgaccgtgca gtgcgtggcc ggcgtcgtcg cgttcgcggt cgggcgcatc aaggccgagc
     3781 tgctgccgaa atacccgaac gtggacgacg tggtgggcct ggaacacagc tacggctgcg
     3841 gcgtggcgat cgacgcgccg ggcgccgaga tcccgatccg cacgctgcgc aacatcacgc
     3901 tcaacccgaa cttcggcaac gaggtgatgg tcgtgagcct gggctgcgag aagctgcaac
     3961 ccgagcggct gctgccgccg ggcagcttcg cgatcgtcgc gaacgacgac gctgcgcgcg
     4021 acgcatcgcc cgagctcgac gtcgtctgcc tccaggacgc gggccacatc ggcttcatgt
     4081 cgatgatcgt ttccatcatg acgacagccg agagccatct cgcgcgcctg aacgcgcggc
     4141 gccgcgaaac ctgcccggcc agcgcgctcg tcgtcggcgt gcagtgcggc ggcagcgacg
     4201 cgttcagcgg cgtcaccgcg aacccggcgg tcggcttctg caccgacctg ctggtgcgcg
     4261 ccggcgcgac cgtgatgttc agcgagacga cggaagtgcg cgacggcata gcccagctca
     4321 cggcgcgcgc ggccacatcc gaagtcgccg acgcgatgat ccgcgagatg gcctggtacg
     4381 acgcctacct cgcgcgcggc cgggtcgacc gcagcgcgaa cacgacgccg ggcaacaagc
     4441 agggcggcct gagcaacatc gtcgagaagg cgatgggctc gatcgtcaag agcggcagcg
     4501 cgccgatcac gggcgtgctg tcgcccggcg agaggttgcg cgatcgcggc atcaccggcg
     4561 gcctcgtcta cgccgcgacg ccggcgagcg acttcatctg cggcaccctg cagctcgccg
     4621 ccggcatcaa cctgcacgtc ttcacgaccg gccgcggcac gccctacggc ctggccgagg
     4681 tgccggtgat caaggtcgcg acgcgcagcg acctggcgcg ccgctggcac gacctgatgg
     4741 acgtcaacgc cgggcgcatc gccgacggca cggccagcat cgacgaggtc ggctgggagc
     4801 tgtttcggct gatgctcgag gtggccagcg gccgcaggaa gacctgggcc gagcactgga
     4861 agctgcacaa cgcgctggtg ctgttcaacc cggcgccggt gacctgagcg ccacgcggcg
     4921 atactgcaga ccccgcgcga atgccttccg actcatgccg cccatcatct ccgtcgccga
     4981 tgtctccaag acctacgcct ccggtttccg cgcgctcgac catgtcagcc tggaggtcca
     5041 gcccggcgag atcttcgcgc tgctcggccc gaacggcgcc ggcaagacca cgctgatcgg
     5101 cctcatctgc ggcatcgtca agctcggcga aggctcggtg aaggtcgcgg gcctcgacat
     5161 cgtcgccgac taccgcgcgg tgcgctcgat gatcggcctg gtgccgcagg agctcacgac
     5221 cgacgcgttc gagaccgtct gggcgacggt ctcgttcagc cgcgggctgt tcggcaagcc
     5281 ggtcgacccg gcgtacaccg aaaagacctt gcgcgcgctg tcgctgtggg acaagaagga
     5341 cagcaagatc atgacgctgt cgggcggcat gaagcgccgc gtgatgatcg ccaaggcgct
     5401 gtcgcacgag ccgcgcgtgc tgttcctcga cgagccgacc gccggcgtcg acgtggcgct
     5461 gcgcaaggac atgtgggcga tggtgcgctc gctgcgcgag tcgggcgtga cgatcgtgct
     5521 gaccacgcat tacatcgagg aggccgaaga catggccgac cgcgtcggcg tgatcagcca
     5581 cggcaagatc atcctcgtcg aggacaaggt cacgctgatg cacaagctcg gcaagaagca
     5641 gctggtgctg caactgcagc agccgctggc ccgcctgccc gagagcctgg cggcgcacgc
     5701 gctgaacctg aacgcgcagg gcaccgaact gacctacgtg tacgacccgc gggcgtcgcg
     5761 caacgacgtc gcggcgctgc tcgacgatct gcgccgcacc ggcatcgggc tgcgcgacct
     5821 gcagacgacg cagagctcgc tcgaggacat cttcgtcagc ctggtggagg gcgcccgatg
     5881 aacctgcacg cgatccgcgc gatctacgcc ttcgagatgg cgcgcacctg gcgcaccatc
     5941 acgcagagca tcatctcgcc ggtgctgtcg acggcgctgt acttcgtcgt gttcggctcg
     6001 gcgatcggct cgcgcatcga cagcatcggc acggtcagct acggcgcctt catcgtgccg
     6061 gggctgatca tgctgtcggt gctgacgcag agcatctcga acgcatcgtt cggcatctac
     6121 ttcccgaagt tcaccggcac gatctacgaa ctgctgtcgg cgccgatctc gcacgtcgag
     6181 atcgtgctga gctacgtcgg cgcggcggcg accaagtcgg tgctggtcgg gttgatcatc
     6241 ctcgggaccg cggcgctgtt cgtgccgatc cacatggcgc acccggtctg gatgctgctg
     6301 ttcctggtgc tgacggcggt gaccttcagc ctgttcggct tcatcatcgg catctgggcc
     6361 gacgacttcg agaagctgca gctgatcccg ctgctgatcg tgatgccgct gacctttctc
     6421 ggcgggccgt tctactcgat cagcatgctg ccgccgctgt ggcagaagct gtcgatgctg
     6481 aacccctcgg tctacctgat cagcggcttc cgctggagct tcttcggcgt cgcggagatg
     6541 cacgtgggca tcagcttctc ggtgacgctg ctgttcttcg tggtggcgct gtggttcgcc
     6601 acctggatca tgcgttcggg ctggcggctc aaggcctgaa cgcgcgtcca ccgtgcagga
     6661 cgtaggacgc gaccgacaaa cgatcgcgca gcgcgccgct gggccgcgcg catcctccga
     6721 cctatcctgc aacggtctca atcgtcagtg ggtgaagata tgaagcgact caaagtgatc
     6781 ctgatcgccg cgatgctcgg cgccacctgc ggggcagcat tcgccgccga gccgggctcg
     6841 gacgccgagc gccgcgaacg catggaccgc gcctacgagg actaccgcaa ccaggccccc
     6901 ggccccgcgg cacgcaccga gagctcgatc aagcgcggtg cccaccgcgc cggcagcgcg
     6961 atcaagcatg gcgcgcagaa ggtcggccac gcggtcggca agggcgtgcg caagaccggc
     7021 gaggcgatcg gccacggcgg cgaaaaactc gaggacaagt cgacgcccaa gccgtagcat
     7081 gatccgcgtg gcgcaacgcg ggagtcggct catgaaggtg ttcgcggtct cgaaggtgcg
     7141 gctcgatgcc gacgggcgca tcacgcacgt ctgctggggc ccggtcgaca cgcgcaagaa
     7201 cgactgggcc gccgcggaga cggtggcgcc ggtggccgac gcggtgcgcg cgatccacga
     7261 cgggcacaag gtctacgcgc tgtttccgtc gacccacggg cacctgcccg accggccgtt
     7321 catggtcgt
//
LOCUS       DATMJB010000052         2692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_642807, whole genome shotgun sequence.
ACCESSION   DATMJB010000052 DATMJB010000000
VERSION     DATMJB010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642807"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2171)
                     /locus_tag="VNU48_01245"
     CDS             complement(<1..2171)
                     /locus_tag="VNU48_01245"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWI09935.1"
                     /translation="MKAPTRSPAEQLAPGRALTFANVAEGAEGLVVSDLARAVAARPK
                     PSAVSLAVICRDGPRMQQLARALEFFSPDLPVMQFPAWDCQPYDRVSPHGGILAQRLT
                     TLARLSRLTGSDKPLIVLTTVNAIVQRVPARELVAAQALSVAPGHVVPMDSIIAWLEH
                     NGYNRSSTVREPGEYAVRGGILDLFPAGLEQPVRFDFFGDSLESIRTFDAETQRTLLD
                     MRALDLVPISEFQLVTETIRRFRMGYVATFGAPERDDPLYEAVSEGRRHPGMEHWLPL
                     FSERMDTLFDYLDGAPVAIEPQSEDAARERFKQIIDYYDARREALEHPGSGAIYKPLP
                     PDRLYLTEDEWAKRLGDAALARLTPFAMPEGSAGVVDAGARQGRNFAPERTDGSVNVF
                     EAVVAHVGALQAQRKKVVIALWSEGSRDRMGSMLKDHKLSNLTSVNSWRTVQATPRNE
                     AMLAVVGMESGFETDNVAVISEQDILGDRLVRPRKASRKLDNFISEVTGLTTGDLVVH
                     VEHGIGRFVGLQTIEVGGAPHDCLELHYAAETKLFLPVENIELLSRYGSDHANVELDR
                     LGGGGWQARKAKLKNRIREIAGELIKIAAERHLHEAPKMPVQPHVYDEFCARFPYEET
                     EDQLGAINATLKDLEIGRPMDRLICGDVGFGKTEVALRAAFAVALEGKQVAVVVPTTL
                     LARQHTRNFTERFRGFPVNIAQASRLVAPKELSQVKKNLADG"
     gene            complement(2168..2455)
                     /locus_tag="VNU48_01250"
     CDS             complement(2168..2455)
                     /locus_tag="VNU48_01250"
                     /inference="COORDINATES: protein motif:HMM:NF015870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HWI09936.1"
                     /translation="MTGSTRSSDGLDDRRKRLLFRCWHRGTREMDLILGRFADAEIAN
                     LPDAELAQLELLMDVPDPDLYAAFTGDAPLAPEYASGLFDRIKSFRAGFEA"
BASE COUNT          465 a          889 c          871 g          467 t
ORIGIN      
        1 ccgtcggcaa ggttcttctt tacctgcgac agttccttcg gcgcgaccag gcgcgaggcc
       61 tgcgcgatat tgaccgggaa gccgcggaaa cgttcggtga aattcctggt gtgctggcgc
      121 gccaacagcg tggtcgggac cacgaccgcg acctgcttgc cttcgagcgc taccgcgaag
      181 gccgcgcgca gcgccacctc ggtcttgccg aagccgacat cgccgcagat cagccggtcc
      241 atcggccggc cgatttcgag gtccttcagg gtggcgttga tagcgcccag ctgatcttcg
      301 gtttcctcgt aagggaagcg cgcgcagaac tcgtcataga cgtgcggctg caccggcatt
      361 ttcggtgcct catgcagatg ccgctcggcg gcgatcttga tcaattcgcc ggcgatctcg
      421 cgtatccggt tcttgagctt ggccttgcgc gcctgccagc caccgccgcc gagcctgtcg
      481 agttcgacat tggcgtgatc ggaaccgtag cgcgacagca gttcgatatt ctcgacaggc
      541 agaaacagtt ttgtttccgc tgcataatgc agctcgaggc agtcgtgcgg cgcgccgccg
      601 acctcgatcg tctgcaaccc gacgaagcgg ccgatgccgt gctcgacatg gaccacgagg
      661 tcgccggtgg tcagaccagt gacttcggag atgaaattat cgagcttgcg gctggctttg
      721 cgcggacgaa ccagccggtc gccgagaatg tcctgctcgc tgatgacggc gacattgtcg
      781 gtctcgaaac cggattccat gccgaccacg gccagcatcg cctcgttgcg cggcgtggcc
      841 tgcaccgtgc gccagctgtt gacgctggtg agattggata gcttgtggtc tttcagcatg
      901 ctgcccatgc ggtcgcgcga gccttcgctc cacagcgcga tcaccacttt cttgcgctgc
      961 gcctgcaacg cgccgacatg ggccacgacg gcttcgaaca cgttgacgga accgtcggtg
     1021 cgctccggcg cgaaattgcg tccctgccgg gcaccggcat cgaccacgcc ggcgctgccc
     1081 tcgggcatgg cgaacggcgt cagccgcgcc agcgcggcgt caccgaggcg tttggcccac
     1141 tcgtcctcgg tcaggtacag ccggtcgggc ggcagcggct tgtagatcgc gccggagccg
     1201 ggatgctcca gcgcctcacg ccgcgcatcg tagtaatcaa ttatctgctt gaagcgttcg
     1261 cgcgccgcgt cctcgctttg cggttcgatt gcgacaggcg cgccgtcgag atagtcgaac
     1321 agcgtatcca tccgctccga aaacagcggc agccagtgct ccatgccggg atggcggcgg
     1381 ccttcgctga ccgcctcgta caagggatcg tcgcgctccg gcgcgccgaa ggtcgcgaca
     1441 tagcccatcc ggaagcgccg gatggtctcg gtgaccagtt gaaattccga gatcggcacc
     1501 agatcgagcg cgcgcatgtc cagcagcgtg cgctgggttt ccgcatcgaa ggtccggatc
     1561 gattccaggc tatcgccgaa gaagtcgaac cgcaccggct gctcgagccc ggccggaaac
     1621 aggtcgagaa tgccgccgcg cacggcgtat tcgccgggct cgcgcaccgt cgacgagcgg
     1681 ttgtagccat tgtgctccag ccaggcgatg atggagtcca tcggaacgac atggccgggc
     1741 gccaccgaca gcgcctgcgc cgcgacgagc tcgcgcgccg gcacccgctg cacgatggcg
     1801 ttgaccgtcg tcagcacgat cagcggcttg tcgctgcctg tcaggcgcga cagccgcgcc
     1861 agcgtggtca gccgctgcgc caggatgccg ccatggggcg atacccggtc atagggctgg
     1921 cagtcccagg ccgggaactg catgacaggc aaatcgggcg agaagaactc cagtgcgcgg
     1981 gccagttgct gcatgcgcgg gccgtcgcgg cagatcacgg caaggctcac ggccgacggt
     2041 tttggacgcg ccgccaccgc gcgggcgaga tccgagacca caaggccctc ggcgccttcg
     2101 gcgacattgg cgaacgtcag cgcgcgcccg ggcgcaagct gctcggcggg agaccttgtc
     2161 ggcgccttca tgcctcgaaa cctgcgcgaa acgatttgat gcggtcgaac agcccgctgg
     2221 catattccgg cgccagcggc gcatcgccgg tgaacgcggc ataaaggtcc gggtccggca
     2281 cgtccatcag cagttcgagc tgcgccagtt ccgcgtcggg caggttggcg atctcagcgt
     2341 cggcaaaccg gccgaggatc aggtccatct cgcgggtgcc gcgatgccag cagcgaaaca
     2401 acagccgctt gcggcggtcg tcgaggccgt cgctcgatcg tgtcgatccc gtcattgtcc
     2461 caatcccatc cgaacgccaa aagcccggac gtgccgggcg gggttgatat agcgacggat
     2521 cggatgaatg tcagccccaa ctgtcccgtg aatcgccaac accgccggcc atcgacgtct
     2581 tgatttcaaa ccgacgacaa aaaagacgtg gatgcctggc acaaagccgg gcatgacggc
     2641 tgtgaacggt cctaggtttc gaaatgcgcc ctgctctgct caaccccctg tt
//
LOCUS       DATMJB010000053         2559 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_701941, whole genome shotgun sequence.
ACCESSION   DATMJB010000053 DATMJB010000000
VERSION     DATMJB010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2559
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701941"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VNU48_01255"
     CDS             <1..280
                     /locus_tag="VNU48_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09937.1"
                     /translation="KEYRLVAWPHLRPPFDRIAYRRMLSDMSHRHMTLAQLSDCSGIR
                     RHDVRAFLDMLEARHLVVQREAAAADSLFSLRPLGGWLRRALAAAHLD"
     gene            complement(290..985)
                     /gene="kdpE"
                     /locus_tag="VNU48_01260"
     CDS             complement(290..985)
                     /gene="kdpE"
                     /locus_tag="VNU48_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HWI09938.1"
                     /translation="MSEPRPTIVVVEDEPQIRRFVRAALEAEHCQVFEAATLKQGLVE
                     AGTRRPDLVILDLGLPDGDGVDYLRDLRAWSQVAVIVLSARIDEADKIAALDAGADDY
                     LTKPFGVGELLARVRVALRRGQRAGESGEARYSFGDVTVDFAARRVERAGAVVHLTPI
                     EYRLLGVLIANAGRVLTHRALLREVWGPAHAERTHYLRVYMGQLRQKLEADAAQPRRI
                     LTETGVGYRLLAD"
     gene            complement(982..>2559)
                     /locus_tag="VNU48_01265"
     CDS             complement(982..>2559)
                     /locus_tag="VNU48_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HWI09939.1"
                     /translation="PPAERAAPASAFAPRARIGYLWAAAACGLTTLVALPLQPIFDLA
                     NIVMLFLLAVVGVAVRFGRGPAVLAAFLNVAAFDFFFVPPHLSFAVSDVQYLLTFAVM
                     LVVGLVTGQLTAGLRFQARVATYREARSRALFEFARELSSVLLAPQCVEIAERAIGRE
                     FHARAQVFVLDLQERLRMPLSAPADATLDIGTAQWALDHAEAAGLGTDTLPGSAWLYL
                     PLKAPMRARGVLALQPEQARLLLVPEQRQQLETFALLTAIALERVHYVEVAQQATLQI
                     ESERLRNSLLSALSHDLRTPLAALYGLADTLVASTPPLLPQPREIAQALATEVRRLNA
                     MVNNLLDMARLQSGVVQLRRDWLPIEEVVGSALQSVAAVLTSHHVHTALPHDLPLVNF
                     DAVLIERVLANLLENAAKYTPPGSALTIAARVNGADLQVSVSDDGPGIPAGREEALFE
                     KFARGERESATAGVGLGLAICRAIVQAHGGRIWAESAGLPAPRPGARLCFTLPLGEPP
                     LMPAAEEAEDAVASALR"
BASE COUNT          379 a          958 c          895 g          327 t
ORIGIN      
        1 gaaggaatac cgcctcgtcg cgtggccgca cttgcggccc ccgttcgatc gcatcgccta
       61 ccggcgcatg ctgagcgaca tgtcgcaccg ccacatgacg ctggcgcagc tgagcgactg
      121 cagcggcatc cggcgccacg acgtgcgcgc gtttctggac atgctcgagg cgcgccacct
      181 ggtcgtgcag cgcgaagccg cggccgccga ttcgctgttc tcgctgcgcc cgctgggcgg
      241 ctggctgcgc cgggccctgg ccgccgccca cctggactga tcgcgagcct cagtcggcga
      301 gcagccggta gccgacgccg gtctcggtca ggatgcgacg cggttgtgcc gcatccgcct
      361 ccagcttctg gcgcagctgg cccatgtaga cgcgcaggta gtgggtgcgc tcggcatgcg
      421 ccgggcccca gacttcgcgc agcaacgcac ggtgcgtcaa cacccgcccc gcgttcgcga
      481 tcagcacgcc gagcaaccgg tactcgatcg gtgtcagatg caccaccgcg ccggcacgtt
      541 cgacacgacg ggccgcgaag tcgaccgtga cgtcgccgaa gctgtagcgc gcctcgcccg
      601 actcgcccgc gcgctggccg cggcgcaagg ccacgcgcac gcgcgccagc agttcgccga
      661 cgccgaaggg cttggtcagg tagtcgtcgg cgccggcatc gagcgccgcg atcttgtcgg
      721 cctcgtcgat gcgcgccgac agcacgatca ccgccacctg cgaccaggcg cgcaggtcgc
      781 gcaggtagtc gacgccatca ccgtccggca gcccgaggtc caggatcacc aggtccgggc
      841 ggcgcgtgcc ggcctcgacc aggccctgct tcagggtcgc ggcctcgaag acctggcagt
      901 gctcggcctc gagcgcggcg cgcacgaagc gccggatctg cggttcgtcc tcgacgacga
      961 cgatggtggg gcgcggctcg ctcatcgcag cgcagacgcc accgcgtctt ccgcttcctc
     1021 ggccgccggc atcagcggcg gctcgcccag cggcagcgtg aagcacagcc gcgcgcccgg
     1081 tcgcggcgcc ggcagcccgg ccgactcggc ccagatgcgc ccgccgtgcg cctgcacgat
     1141 cgcgcgacag atcgccaggc ccaggccgac ccccgcggtc gccgactcgc gctcgccgcg
     1201 cgcgaacttc tcgaacagcg cctcctcgcg cccggccgga atgcccgggc cgtcgtcgct
     1261 gacgctgacc tgcagatcgg ccccgttcac gcgcgccgcg atcgtcagcg cgctgcccgg
     1321 cggcgtgtac ttcgcggcgt tctcgagcag gttcgcgagc acgcgctcga tcagcaccgc
     1381 atcgaagttg accagcggca ggtcgtgcgg cagcgccgtg tgcacgtggt gagaggtgag
     1441 cacggcggcg accgattgca gcgcactgcc caccacttcc tcgatcggca gccagtcgcg
     1501 cctgagctgc accacgccgc tttgcagccg cgccatgtcg agcaggttgt tgaccatcgc
     1561 gttcaggcgc cgcacctcgg tggcgagcgc ctgggcgatc tcgcgcggct gcggcagcag
     1621 cggcggcgtg ctcgccacca gcgtgtcggc cagaccgtac agcgccgcca gcggcgtgcg
     1681 caggtcgtgc gacagcgccg acagcagcga attgcgcagg cgttccgatt cgatctgcaa
     1741 cgtcgcctgt tgtgccacct cgacatagtg cacgcgctcg agcgcgatcg cggtcagcaa
     1801 tgcgaaggtc tcgagctgct gacgctgctc cggcacgagc agcaggcggg cctgctcggg
     1861 ctgcagcgcg agcacgccgc gcgcgcgcat cggcgccttc agcggcaggt acagccaggc
     1921 gctgccgggc aaggtgtcgg tgcccaggcc ggccgcttcg gcgtggtcca gcgcccactg
     1981 cgcggtgccg atgtccagcg tcgcgtcggc cggggcggac agcggcatgc gcagccgctc
     2041 ctgcaggtcg agcacgaaca cctgcgcgcg ggcgtggaac tcgcggccga tcgcgcgctc
     2101 ggcgatctcg acgcactgtg gcgccagcaa cacgctcgag agttcacgcg cgaactcgaa
     2161 cagcgcgcgc gagcgggctt cgcgataggt tgccacgcgc gcctggaagc gcaggccggc
     2221 ggtgagctgc ccggtgacca ggccgaccac caacatcacc gcgaaggtca gcaggtactg
     2281 cacgtccgag accgcgaacg acaggtgcgg cggcacgaag aagaagtcga acgcggcgac
     2341 gttcaggaac gccgcgagca ccgccggccc gcgcccgaag cgcaccgcca cgccgaccac
     2401 cgcgagcagg aacagcatca cgatgttcgc gagatcgaag atcggctgca gcggtagcgc
     2461 aaccagcgtc gtcaggccgc acgccgccgc cgcccacagg tagccgatgc gggcgcgcgg
     2521 cgcgaacgcg ctcgcgggcg ccgcacgctc ggccggcgg
//
LOCUS       DATMJB010000054         3116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_346875, whole genome shotgun sequence.
ACCESSION   DATMJB010000054 DATMJB010000000
VERSION     DATMJB010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346875"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VNU48_01270"
     CDS             complement(<1..794)
                     /locus_tag="VNU48_01270"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWI09940.1"
                     /translation="MKPIQVGLLGIGTVGGGTFNVLQRNQEEIQRRAGCGIRIAMVAD
                     LDTERARRIVGDSARIVSDAREIIANPEIDIVIELIGGYGVAKVLVMEAIAAGKHVVT
                     ANKALLAVHGTEIFAAARAKGVVVAFEAAVAGGIPIIKALREGLTANRIEWIAGIING
                     TTNFILSEMRSKGLDFDVALKEAQRLGYAEADPSFDIEGVDAAHKATIMSAIAFGIPV
                     QFDKAYVEGITKLNATDIRYAEQLGYRIKLLGITKRRDDAGGIELRV"
     gene            complement(825..2051)
                     /locus_tag="VNU48_01275"
     CDS             complement(825..2051)
                     /locus_tag="VNU48_01275"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWI09941.1"
                     /translation="MRPIVKSSKLANVAYDIRGPVLDKARQMEEEGHKIIKLNIGNVA
                     AFGLEPPDEIVQDMIRNLPHSAGYTDSKGLFAPRKAVVHYTQQKHVAGVTIDDVYLGN
                     GASELIAMGMNALLNDGDEVLIPSPDYPLYTAVVGLSGGTPVHYRCDESTGWLPDIEH
                     MKAMITPATRALVVINPNNPTGALYPPELLREIVEVARTHQLIVFADEIYDKTLYDGN
                     THTSIASLADDVLFVTVNGLSKNYRSCGYRSGWMVVSGEKRHARDYIEGLNMLASLRL
                     CANTPGQLAIQTALGGYQSIDDLVAPGGRLCRQRDLAYQLLSEIPGVSVVKPKAALYM
                     FPRLDPKVYPIADDQQFAYDLLSEQKVLIVQGTGFNWATPDHFRIVFLPNSDDLTEAI
                     GRIARFLEHYRKRHSH"
     gene            2164..2559
                     /locus_tag="VNU48_01280"
     CDS             2164..2559
                     /locus_tag="VNU48_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HWI09942.1"
                     /translation="MKLQADRIEGQNAIARHGSEGVLVNGVEHRRSVIVPWTGAVAPW
                     PVDRFEALTPAHFADLLALAPELVIFGSGAKIRFVRPALLKALIERRIGVETMDTAAA
                     CRTYNVLLAEGRSVVAALLFEPAPAAGQG"
     gene            2638..3114
                     /locus_tag="VNU48_01285"
     CDS             2638..3114
                     /locus_tag="VNU48_01285"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWI09943.1"
                     /translation="MTPALNKPLPEFEALATGGVKFTPQAFAGKIVILYFYPKDNTPG
                     CTTEAMQFRDHHKEFVKAGAVVFGVSRDNMASHDKFKQSLELPFELIADTEEKLCHMF
                     GVVKNKIMYGKKVKGIERSTFLVGADGILKQEWRGLKVPGHVDEVLKAVKLLKKAA"
BASE COUNT          522 a          987 c         1048 g          559 t
ORIGIN      
        1 acgcgcagct cgatgccgcc ggcgtcgtca cgccgcttcg tgatcccgag cagcttgatc
       61 cggtatccga gctgctcggc atagcggatg tcggtcgcat tcagcttcgt gatgccttcg
      121 acgtaggcct tgtcgaactg caccgggatg ccgaacgcga tcgcgctcat gatcgtcgcc
      181 ttgtgcgcgg cgtcgacgcc ctcgatgtcg aagctcgggt cggcctcggc gtagcccaga
      241 cgctgggcct ccttcagcgc gacgtcgaag tcgagcccct tcgatcgcat ctcggaaagg
      301 atgaaattgg tggtgccgtt gatgatgccg gcgatccact cgatgcggtt ggcggtcagg
      361 ccctcgcgca gcgccttgat gatcgggatg ccgccggcca ccgcggcctc gaacgcgacg
      421 accacgccct tcgcacgcgc cgcggcgaag atctcggtgc cgtgcaccgc cagcagcgcc
      481 ttgttcgcgg tgaccacgtg cttgccggcg gcgatcgcct ccatcaccag caccttggca
      541 acgccgtagc cgccgatcag ctcgatgacg atgtcgatct cgggattggc gatgatctcg
      601 cgcgcgtcgc tgacgatccg cgccgagtcg ccgacgatgc gccgcgcccg ttcggtgtcc
      661 agatcggcca ccatcgcgat gcgaatgccg caaccggcgc ggcgctggat ctcttcctga
      721 ttgcgttgca gcacgttgaa ggtgccgccg ccgacggtgc cgatgccgag caggcccact
      781 tgaatcggtt tcatggtgca gtttcgaaaa gcttcggtgg ggagtcagtg ggaatggcgc
      841 ttgcggtagt gctcgaggaa gcgcgcaatg cggccgatcg cctcggtcag gtcgtcggag
      901 ttgggcagga acacgatgcg gaagtggtcc ggcgtggccc agttgaagcc ggtgccctgc
      961 acgatcagca ccttctgctc cgagagcagg tcgtaggcga actgctggtc gtcggcgatc
     1021 gggtagacct tggggtcgag cctggggaac atgtacagcg ccgccttcgg cttgacgacg
     1081 ctgacacccg ggatctcgct cagcagctgg tacgccagat cgcgctgccg gcacagacgc
     1141 ccgccgggcg cgaccaggtc gtcgatgctc tggtagccgc cgagcgccgt ctggatcgcg
     1201 agctggcccg gcgtgttcgc gcacaggcgc agcgaggcca gcatgttcag cccctcgatg
     1261 tagtcgcgcg cgtggcgctt ctcgcccgat acgaccatcc agccggaacg gtagccgcag
     1321 gaacggtagt tcttcgacag gccgttgacg gtgacgaaca gcacgtcgtc ggcgagcgag
     1381 gcgatgctgg tgtgggtgtt gccgtcgtac agggtcttgt cgtagatctc gtcggcgaac
     1441 acgatcagct ggtgcgtgcg ggcgacctcg acgatctcgc gcagcagctc tggcgggtac
     1501 agcgcaccgg tcgggttgtt cgggttgatc acgaccagcg cgcgcgtggc cggcgtgatc
     1561 attgccttca tgtgctcgat gtcgggcagc cagccggtcg attcgtcgca gcggtagtgc
     1621 accggcgtgc cgcccgacag gccgacgacc gccgtgtaca gcggatagtc gggcgacggg
     1681 atcagcacct cgtcgccgtc gttgagcagc gcgttcatgc ccatcgcgat cagctcggag
     1741 gcgccgttgc cgaggtacac gtcgtcgatc gtcacgccgg cgacatgctt ttgctgggtg
     1801 tagtgcacca ccgccttgcg cggcgcgaac aagcccttgc tgtcggtgta gcccgccgag
     1861 tgcggcaggt tgcggatcat gtcctgcacg atctcgtcgg gcggctccag gccgaacgcg
     1921 gcgacgttgc cgatgttcag cttgatgatc ttgtggccct cttcttccat ctggcgcgcc
     1981 ttgtccagca cgggcccgcg gatgtcgtag gccacgttgg cgagcttgct cgacttgacg
     2041 atgggtctca aaaggcctcc tggggagttg ctgcgacgca aaacatagaa tttaaccaca
     2101 gggattcgcg cgtacgaccc atgtacgtgc cctggcaata cccgagaccg tcccgccacc
     2161 gccatgaaac tccaagccga ccgcatcgaa ggccagaacg cgattgcccg ccacggcagc
     2221 gaaggggtgc tcgtcaacgg tgtcgaacat cgtcgcagcg tgatcgtgcc ctggaccggt
     2281 gcggtggcgc catggccggt cgaccgcttc gaggcgctca cgccggcgca tttcgccgat
     2341 ctgctggcgc tggcgccgga actggtgatc ttcggcagcg gtgcgaaaat ccgcttcgtg
     2401 aggccggcct tgctgaaggc gctgatcgag cgccgcattg gcgtcgagac gatggacacc
     2461 gcggcggcct gccgcaccta caacgtgctg ctggccgagg gccgatcggt ggtggcggcg
     2521 ctgctgttcg agccggcgcc ggccgccggc cagggttgag cggcgcgtcg ggcgcgcccc
     2581 ggcaacgttt ataatctggg gctacgcctg caccggcgca accaatcaga atactccatg
     2641 acgccagccc tcaacaaacc cctcccggaa tttgaagcgc tcgcgactgg tggcgtgaaa
     2701 ttcacgccac aagccttcgc gggcaagatc gtcattctgt atttttaccc gaaggacaac
     2761 accccaggct gcaccaccga ggcgatgcag ttccgtgacc accacaaaga gttcgtgaag
     2821 gccggcgcgg tggtgttcgg cgtctcgcgc gacaacatgg cctcgcacga caagttcaag
     2881 cagagcctgg agctgccttt cgagctgatc gcggacaccg aagagaagct gtgccacatg
     2941 ttcggcgtgg tcaagaacaa gatcatgtac ggcaagaagg tgaagggcat cgagcgcagc
     3001 acgttcctgg tgggcgccga cggcatcctc aagcaggaat ggcgcggcct gaaggtgccg
     3061 gggcacgtgg acgaagtgct caaggccgtg aagctgctga agaaggcggc ctgagc
//
LOCUS       DATMJB010000055         4300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_642562, whole genome shotgun sequence.
ACCESSION   DATMJB010000055 DATMJB010000000
VERSION     DATMJB010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642562"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VNU48_01290"
     CDS             <1..1117
                     /locus_tag="VNU48_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWI09944.1"
                     /translation="PAANVTVQPQVSGMLAQVLFKEGQMVRKGELLATIAPEPFQMAL
                     TQATGARLRDEAQLQAARVTLQRYNTLLGQDSIARQDVDTQAALVKQLEGTVTIDRAN
                     EGTARLNLGYTRIVAPVAGRVGLRPVDAGNYVTTGTTNGIALITQVAPIDVTFAIPQD
                     RVPEIQARVAAGVQLPVAAWDRARTRRLDAGTFSTLDNQIDTTTGTVKAKARFANVDG
                     NLFPNQFVNARLLLRTIAGATVVPVMALRHGPNGDFVYVVNEDKTVALRSVTRGEAGV
                     DDVAIATGLELGEQVVTEGGDRLKDGARVQTSVDRPAGAAFGAASGARGRRGNGASGA
                     FDGGGASAAQGASGASGAFGHRRRASDADAAAASAPR"
     gene            1158..4181
                     /locus_tag="VNU48_01295"
                     /pseudo
     CDS             1158..4181
                     /locus_tag="VNU48_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833811.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(4221..4292)
                     /locus_tag="VNU48_01300"
                     /pseudo
     CDS             complement(4221..4292)
                     /locus_tag="VNU48_01300"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          663 a         1496 c         1509 g          632 t
ORIGIN      
        1 gccggccgcg aacgtgacgg tccagccgca ggtctcgggc atgctcgcgc aggtgctgtt
       61 caaggaaggg cagatggtgc gcaagggcga gctgctcgcg acgatcgcgc ccgagccgtt
      121 ccagatggcg ctgacgcagg ccaccggcgc gcgcctgcgc gacgaggcgc agctgcaggc
      181 ggcgcgcgtc acgctgcagc gctacaacac gctgctcggc caggactcga tcgcgcgcca
      241 ggacgtcgac acccaagccg cgctcgtcaa gcagctcgag ggcaccgtca cgatcgatcg
      301 cgccaacgaa ggcacggcgc ggctgaacct gggctacacg cgcatcgtcg cgccggtcgc
      361 cgggcgcgtc ggcctgcggc cggtcgacgc cggcaactac gtcaccaccg gcacgaccaa
      421 cggtattgcg ctgatcacgc aggtcgcgcc gatcgacgtc acgttcgcga tcccgcagga
      481 ccgcgtgccc gagatccagg cgcgtgtggc cgccggcgtg cagctgccgg tcgcggcctg
      541 ggaccgcgca cgcacgcgcc ggctcgacgc cggcacgttc tcgaccttgg acaaccagat
      601 cgacaccacc accggcaccg tgaaggccaa ggcgcgtttc gcgaacgtcg acggcaacct
      661 gttcccgaac cagttcgtca acgcgcggct gctgctgcgc acgatcgccg gcgcgaccgt
      721 ggtgccggtg atggcgctgc gccacggccc gaacggcgat ttcgtctacg tcgtgaacga
      781 ggacaagacc gtcgcgctgc gcagcgtcac gcgcggcgag gccggtgtcg acgatgtcgc
      841 gatcgccacc gggctggagc tcggcgagca ggtcgtcacc gaaggcggcg atcggctgaa
      901 ggacggtgcg cgcgtgcaga ccagcgtcga ccggccggcc ggtgcggcgt tcggtgcggc
      961 ctcgggcgcg cgcgggcggc gcggcaacgg tgcgagcggc gcgttcgacg ggggcggtgc
     1021 cagcgcggcg cagggggcga gcggcgcgtc cggcgcgttc ggccatcgcc gccgcgcttc
     1081 cgacgccgac gcggcggccg cgagcgcgcc gcgttgaagc cgcgggtgag caagcaccag
     1141 gtcgcgaggc tgccgtcatg agcccgtcac gcccgttcat cgagcggccc gtcgcgacct
     1201 cgctgctgat gctggcgatc gtgctcgcgg gcatggtcgg cttcaggttc ctgccgctgt
     1261 cggcgctgcc gcaggtcgat tacccgacga tccaggtcca gacgctctac cccggcggca
     1321 gccccgaggt gatgagcaac accgtcaccg cgccgctgga gcgccagttc ggccagatgg
     1381 cggggctgga ccggatggcc tcgacgagcg cggccggcgt gtcgatcatc accttgcagt
     1441 tcacgctcgg cctgacgctc gatgtggccg agcaggaggt gcaggccgcg atcaacgccg
     1501 gcggctcgct gctgccggcc gacctgcccg caccgccggt ctacgccaag gtcaacccgg
     1561 ccgacgcgcc ggtgctgacg ctggcgatca cgtccgacgc gatgccgctg accgaggtgc
     1621 agaacctggt caacacgcgg ctggcgcaga agatcagcca ggtgtcgggc gtcggcctgg
     1681 tgtcgctgtc gggcgggcag cgtccggcgg tgcggatcca ggccgacacg cagtcgctcg
     1741 cgtcctatgg catcggcctg gacaccttgc gcactgcgat cacgagcgcg aactcgaacg
     1801 ccgcgaaggg cagcttcgac ggcccgaagc gcgcctacac gatcaactcg aacgaccagc
     1861 tcgtgacggt cgacgactac aagaacctga tcgtcgcgta caagaacggc gcgccgatcc
     1921 gcatgatcga cgttgcccgc gtcgtcaaca gcgccgagaa caagcagctc ggcgcctggg
     1981 ccggcgccgg gcagacgctc acgcccgcga tcatcatgaa cgtgcagcgt caaccgggcg
     2041 cgaacgtgat cgccacggtc gacgcgatca agaagcagct gccgagcctg caggccgggc
     2101 tgccggcggc gctgaagatc gaggtgctgt cggaccgcac gaacggcatc cgcgcctcgg
     2161 tcgagcatgt cgagatggag ctgatcctcg cggtcgttct ggtggtgctg gtgatcttcg
     2221 cgttcctcgg ctccttgcgc gccaccgtga tcgcgagcat cgcggtgccg atctcgctga
     2281 tcggcacgtt cggactgatg tacctgctcg gctactcgct caacaacctg agcctgatgg
     2341 cgctgaccat cgcgaccggc ttcgtcgtcg acgacgcgat cgtgatgatc gagaacatcg
     2401 cgcgctacat cgaggcgggc gagccgccga tgaaggcggc gctgaccggg gcgacgcaga
     2461 tcggcttcac gatcatctcg ctgacggtct cgctgatcgc ggtgctgatt ccgctgctgt
     2521 tcatgagcga cgtcgtcggt cgcctgttcc gcgagttcgc ggtgacgctg gcgatcacga
     2581 tcctgatctc ggcgctggtg tcgctcacac tcgtgccgat gatgtcggcg cgctggttgc
     2641 atggggcccc cggccgcgag tacgcggcca ccccccgagg gggtgcgggg ccgcttggga
     2701 gtggcccggc gccggccccg cgccgcgatg acgcgccctc gcgcgtgcag cgcttctaca
     2761 gcggcgtgat gcaccgctac gaccgcgcgc tggtctgggt catcgatcac caggcgatca
     2821 cgatggtcgt ggcgctcgcg accttcgcgc tgacggtgct gctctacgtg atgatcccga
     2881 agggcctgtt cccgacgcag gacaccggcc agctgcaggg gcgcatccag gccgcgcagg
     2941 acgtgtcgta cgagcgcatg gcgcagctgc agcagcaggc cgcggccgcg atcctggccg
     3001 accccgatgt cgagaacctg agttcgttcg tcggcgtcga tgcggccaac aacacgatgc
     3061 tgcatgccgg cagcatgctg atcaacctga agaagggcca cggcaaccag caggcgacga
     3121 tggaccgcct gcgcgagcgg gtgcgccagg tggctggcgt gacgctgtac gtgcagccca
     3181 cgcaggacct gacgatcgat gccgagaccg gcccgacgca gtaccgcgtc tcgctcgagg
     3241 gcgtcgacag cgcgaccatc acgctgtgga tgggcaagct gatcgagcgg ctgcagcagg
     3301 cgccgcaggt gcgcaacgtc agcagcgacg ccggcgcgca ggggctggcg gcctacatcg
     3361 acgtgaaccg cgacaccgcc gcgcgcctgg gcgtgaccgc gagttcggtc gacgatgcgc
     3421 tgtacagcgc gttcggtcag cgcatcgtct cgaccatctt caccgagacc aaccagtacc
     3481 gcgtgatcct cgaggcgcgc cccgggctgg tcgcgaatgc gcccgacctg ggcaagctga
     3541 acctgatcag cggcggcggc gcgccgacgc cgctgtcggc cttcgcgcgc atcaccgagc
     3601 agctggcgcc gctgcagatc acccacgtcg cgcagtaccc ggcgagcacg ttgaacttcg
     3661 acaccgcgag cggggtcgcg ctgggcgcgg cggtcgacgc gatccgccag gccgcgaagg
     3721 acatcgcgtt gcccgcgagc gtgacgatga ccttcctcgg cgcgtccggc gcctacgagc
     3781 gatcgctgtc gaaccagctc tggctgatcc tggccgcggt gatctgcgtc tacatcgtgc
     3841 tcggcgtgct gtacgagagt tacgtgcacc cgctgacgat cctgtcgacc ttgccgtcgg
     3901 ccggcatcgg cgcgctgctc gcgctgatga tcgccggcca tgacctgggc gtgatcggca
     3961 tcatcggcat catcctcttg atcggcatcg tcaagaagaa cgcgatcatg atgatcgact
     4021 tcgcgatcga cgcgcagcgc aacgagggca agagcccgcg cgacgcgatc ttccaggccg
     4081 ccatcctgcg cttccgcccg atcctgatga ccaccctggc ggccctgttc gcggccatcc
     4141 cgctgatgct gagcttcggc gagggcggcg aacagcgccg ccgcggtggt catcaggatg
     4201 ggccgcaggc gcagcagcgc ggcctggtgg atggcctcgc gcgggctctt gccctgctcg
     4261 cgctcggcct cgagcgcgaa gtcgatcatc atgatcgcgt
//
LOCUS       DATMJB010000056         2045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_879739, whole genome shotgun sequence.
ACCESSION   DATMJB010000056 DATMJB010000000
VERSION     DATMJB010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879739"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VNU48_01305"
     CDS             complement(<1..286)
                     /locus_tag="VNU48_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09945.1"
                     /translation="MTATPHDIERLFARQDAHQHRVRATSAEQRIAKLQALRAAVVAH
                     LPAAVAAMAEDFGKPGFEAACEVAALLGEIDHVAAHLARWMQPVEVQPSAS"
     gene            complement(297..1409)
                     /locus_tag="VNU48_01310"
     CDS             complement(297..1409)
                     /locus_tag="VNU48_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWI09946.1"
                     /translation="MQIQAALLRHEHADFSVETVDLGAPQADEVLVDIVATGLCHTDL
                     AVRDGVFPFPAMPNVLGHEGAGVVSAVGSAVLDIDVGDHVVLSFAFCGSCAQCDAGRP
                     AYCDNGMGLNVSGRRPDGSCTHHQHGQPINACFFGQSSFASHALVKARHVVKVARDIP
                     LELLGPLGCGIQTGAGTILNSLKATAGSSVAIFGAGAVGMSAIMAAKIAGCATIIAVD
                     VQPTRLALARELGATHVVNGRVADAVACIKEITGAGADFVVEATGLPDVVAQSIRSCR
                     KLGQVALLGVGKMDAPLPLVLGDFMAGMVIRYVLEGDSVPSVFVPQLIEFYRQGRFPF
                     DRLIKFYDLDAINDAARDADAGGTIKPVIRMPRTSA"
     gene            complement(1445..>2045)
                     /locus_tag="VNU48_01315"
     CDS             complement(1445..>2045)
                     /locus_tag="VNU48_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004552478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI09947.1"
                     /translation="EIEGLDHPSVLDYLAVLRDHQPVGGCVAIIGAGGIGFDVATYLA
                     HSGPGAALDADKFYAEWGVDTRPGPHSGLRAPRVEASPRRIHLLQRKASKVGEGLGVS
                     TGWIHRAALKNRGVDMIAGATYRRIDDAGLHISVAGEDRLLAVDHVIVCAGQLPERSL
                     ASGLGRTPGCVHLIGGADEAAELDAKRAINQAVRLAATL"
BASE COUNT          339 a          689 c          696 g          321 t
ORIGIN      
        1 cagaggcgct gggttgcacc tcgaccggct gcatccatcg cgcgaggtgg gccgcgacgt
       61 ggtcgatttc acccagcagc gcagcgacct cgcacgcggc ctcgaagccc ggcttgccga
      121 agtcctcggc catcgccgcc accgcggccg gcaggtgcgc cacgacggcc gcgcgcagcg
      181 cctgcagctt ggcgatgcgt tgctcggccg aggtcgcgcg cacgcggtgc tggtgcgcgt
      241 cctggcgcgc aaacaggcgt tcgatgtcgt ggggggtggc ggtcatggtc gtctcctcag
      301 gcgctggtac gcggcatgcg gatcacgggc ttgatcgtgc cgccggcatc ggcgtcgcgc
      361 gcggcgtcgt tgatggcgtc caggtcgtaa aacttgatca agcgatcaaa cgggaaacgt
      421 ccctggcggt agaactcgat caattgcgga acgaagacgc tgggcacgct gtcgccttca
      481 agcacgtaac ggatcaccat ccccgccatg aaatcgccga gcaccagggg cagcggtgca
      541 tccattttgc ccacgccgag cagggcgacc tggcccaatt tccgacagga acggatgctc
      601 tgggcaacga catcgggcaa gcccgtggct tccaccacga agtcggcgcc ggcaccggtg
      661 atttccttga tgcatgccac cgcatccgcg acacggccgt tgacgacatg cgtggcgcca
      721 agttcgcgtg ccagggcgag gcgtgtgggt tgcacatcga cggcgatgat ggtggcgcag
      781 ccggcgatct tcgccgccat gatggccgac atgccgacgg cgccggcacc gaagatcgcc
      841 acggaactgc cagccgtcgc cttcaagctg ttgagtatcg tgccggcccc cgtctggatg
      901 ccgcagccca gcggtcccag cagttcgagg ggaatgtcgc gcgcaacctt gaccacgtga
      961 cgcgctttga caagcgcatg ggaagcgaac gacgactgac cgaaaaagca ggcgttgatg
     1021 ggttgcccgt gttgatgatg cgtgcacgat ccgtccggac gtcggccgga cacattcaag
     1081 cccatgccgt tgtcgcagta ggccggcctg cccgcatcgc actgcgcgca ggaaccgcag
     1141 aaggcaaagg acaggacgac gtgatcgccg acatcgatgt ccagcacggc ggagccgact
     1201 gcgctgacca cgccggcccc ttcatggccc aggacgttgg gcatggcagg gaaggggaag
     1261 accccgtcgc gcactgccaa gtcggtatgg caaaggccgg tcgccacgat gtccaccagc
     1321 acttcatccg cctgcggggc accgaggtcc accgtttcga cgctgaaatc cgcgtgctca
     1381 tggcgcaaca gcgctgcttg aatctgcatg ggaccatctc ctgaaaagta tttatggagc
     1441 gtttttaaag tgttgccgcg agcctgacgg cctgattgat ggcccgcttg gcgtccagct
     1501 cggccgcctc gtcggcgccg ccgatcaggt gcacgcaccc cggtgtgcgg cccaggccgc
     1561 tggccagcga gcgctcgggg agctggcccg cgcaaacgat cacgtggtcc accgccagca
     1621 gccggtcctc gccggcaacg ctgatgtgca ggccggcgtc gtcgatgcgc cggtaggtcg
     1681 cgccggcgat catgtcgacg ccgcggttct tcagcgccgc gcggtggatc cacccggtcg
     1741 acacgcccag cccttcgccg accttggagg ccttgcgctg cagcagatgg atgcgccggg
     1801 gcgaggcctc gacgcgcggc gcgcgcaggc cgctgtgcgg gccggggcgc gtgtcgacgc
     1861 cccattcggc gtagaacttg tcggcatcga gcgcggcgcc ggggccgctg tgcgccaggt
     1921 aggtcgccac gtcgaagccg atgccgccgg cgccgatgat ggccacgcac ccgcccaccg
     1981 gctggtggtc gcgcagcacc gccagatagt ccagcacgct cggatggtcc agcccttcga
     2041 tctcg
//
LOCUS       DATMJB010000057         3811 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_464724, whole genome shotgun sequence.
ACCESSION   DATMJB010000057 DATMJB010000000
VERSION     DATMJB010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464724"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VNU48_01320"
     CDS             complement(<1..394)
                     /locus_tag="VNU48_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HWI09948.1"
                     /translation="MQPKLLTIVVSAALASTAANAAGVTDQMIQNEATASGNVLTWGV
                     NTQGQRYSPLKQVNTATVGKLAPVWAFSFGGEKQRGQESQPVIHNGKMFVTASYSRIF
                     ALDATTGKKLWKYEHRLPEGIMPCCDVVN"
     gene            complement(463..1983)
                     /gene="adh"
                     /locus_tag="VNU48_01325"
     CDS             complement(463..1983)
                     /gene="adh"
                     /locus_tag="VNU48_01325"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HWI09949.1"
                     /translation="MLYAAPGTPGAKVSFKSQYDNFINGRFVPPVKGQYFDVVTPIDG
                     SVYTKAARSTAEDIELALDAAHAAADAWGRTSAGERANVLLKIADRLEQNLELLAYAE
                     TVDNGKPIRETLNADIPLTVDHFRYFAGCVRAQEGALSEIDENTIAYHFHEPLGVVGQ
                     IIPWNFPILMAAWKLAPALGAGNCVVLKPAESTPVSIMVLAELIADLLPPGVLNIVNG
                     FGREAGMPLAESKRIGKIAFTGSTATGRVIAQAAAANLIPATLELGGKSPNVFFADVA
                     DHDDAFLDKAIEGLVLFAFNQGEVCTCPSRALIHESIYDKFIAKALKRVQAIKQGSPL
                     DTDTMMGAQASAVQMDKILSYMEIGKQEGAQLLTGGARAELGGDLAGGYYIQPTLFKG
                     NNNMRIFREEIFGPVLAVTTFKTEAEALAIANDTPYGLGAGVWTRDSNTAYRMGRGIK
                     AGRVWTNCYHAYPAHATFGGYKESGIGRETHKMMLDHYQQTKNLLVSYSPNKLGFF"
     gene            complement(2374..>3811)
                     /gene="glmS"
                     /locus_tag="VNU48_01330"
     CDS             complement(2374..>3811)
                     /gene="glmS"
                     /locus_tag="VNU48_01330"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWI09950.1"
                     /translation="YDGDLFDAVQRAVVRLRGAFAIAVFCRDEPHRVVGAREGSPLVL
                     GVGRGERAGETFLASDAMALAGVTDQIVYLEEGDVVDLQLGKHWISQRDANGCYRPVQ
                     RELRTVIAHSGAAELGPYRHYMQKEIFEQPKAIADTLDAVPGITPELFGDGAHRVFKD
                     VDSVLILACGTSYYAGSVAKYWLESIAKIPTSVEVASEYRYRDSVPNPRSLVVTITQS
                     GETADTLAALKHARGLGMAHTLTICNVATSAMVRECRFAYITRAGVEIGVASTKAFST
                     QLVGLFLLTLALAQTRGLLTDAQEAGHLTALRHLPLAVQGVLALEPQVIAWSQEFARK
                     ENALFLGRGLHYPIALEGALKLKEISYIHAEAYPAGELKHGPLALVTAEMPVVTVAPN
                     DALLEKLKSNLQEVRARGGELFVFADADTRIVSSEGLHVIRMPEHYGALSPILHVVPL
                     QLLAYHTACARGTDVDKPRNLAKSVTVE"
BASE COUNT          546 a         1243 c         1338 g          684 t
ORIGIN      
        1 gattgacgac gtcgcagcac ggcatgatgc cttcgggcag gcggtgctcg tacttccaga
       61 gcttcttgcc ggtggtcgcg tcgagcgcga agatgcgcga gtacgacgcg gtcacgaaca
      121 tcttgccgtt gtggatcacc ggctgcgatt cctggccgcg ctgcttctcg ccgccaaacg
      181 agaacgccca caccggcgcg agcttgccga cggtggcggt gttgacctgc ttgagcggcg
      241 agtaacgctg gccctgcgtg ttgacgcccc aggtcagcac gttgccactc gcggtggcct
      301 cgttctggat catctggtcg gtgacgccgg cggcgttggc cgcggtcgat gccaacgcgg
      361 cggacacgac aatggtcaag agcttcggtt gcatgttgtc tcctcgaatt gggaatgcgg
      421 tcgaaagggg gcggcatctg ccgccccgtg gctgtgtcgg tgttagaaga acccgagctt
      481 gttcggcgag tagctcacca gcaggttctt ggtctgctgg tagtggtcga gcatcatctt
      541 gtgggtctcg cggccgatgc ccgactcctt gtagccgccg aaggtggcat gcgccggata
      601 ggcgtggtag cagttggtcc agacgcggcc ggccttgatg ccgcggccca tgcggtaggc
      661 ggtgttgctg tcgcgggtcc agacgccggc gcccaggccg tagggcgtgt cgttggcgat
      721 ggccagcgct tcggcctcgg tcttgaaggt ggtcacggcc agcaccgggc cgaagatctc
      781 ctcgcggaag atgcgcatgt tgttgttgcc cttgaacagc gtcggctgga tgtagtagcc
      841 gccggccagg tcaccgccga gctcggcgcg cgcgccgccg gtcagcaact gcgcgccttc
      901 ctgcttgccg atctccatgt acgacaggat cttgtccatc tgcaccgccg aggcctgcgc
      961 gcccatcatc gtgtcggtgt ccagcgggct gccttgcttg atcgcctgca cgcgcttcag
     1021 cgccttggcg atgaacttgt cgtagatcga ttcgtggatc agcgcgcggc tcgggcaggt
     1081 gcagacctcg ccctggttga acgcgaacag caccaggcct tcgatcgcct tgtcgaggaa
     1141 ggcgtcgtcg tggtcggcga cgtcggcgaa gaacacgttc gggctcttgc cgccgagttc
     1201 cagcgtcgcc gggatcaggt tggcggcggc ggcctgtgcg atcacgcggc cggtggcggt
     1261 gctgccggtg aaggcgatct tgccgatgcg cttgctctct gccagcggca tgccggcctc
     1321 acggccgaaa ccattcacga tgttcagcac ccccggcggc agcaggtcgg cgatcagctc
     1381 ggccagcacc atgatgctga ccggcgtgct ctcggccggc ttcagcacca cgcagttgcc
     1441 cgcgcccagc gccggggcca gcttccaggc cgccatcagg atcgggaagt tccacgggat
     1501 gatctggccg accacgccca gcggctcgtg gaagtggtac gcgatggtgt tctcgtcgat
     1561 ctcgctcagg gcgccctcct gcgcccgcac gcagccggcg aagtagcgaa agtggtcgac
     1621 ggtcagcggg atgtcggcgt tcagcgtctc gcggatcggc ttgccgttgt ccaccgtctc
     1681 ggcgtaggcc agcagctcga ggttctgctc gagccggtcg gcgatcttca gcagcacgtt
     1741 cgcgcgttcg ccggccgatg tgcggcccca ggcatcggcc gcggcgtggg cggcgtcgag
     1801 cgcgagttcg atgtcctcgg cggtggagcg cgcggccttg gtgtagacgc tgccatcgat
     1861 gggcgtgacc acatcgaagt actggccctt caccggcggc acgaacctgc cgttgatgaa
     1921 gttgtcgtat tgggacttga acgaaacctt ggcaccaggg gtaccgggag cggcgtagag
     1981 catgggagtc tcctgtgggt gaatgtggaa cgcggacgac cacagagcgc aaacgccgtg
     2041 ccattgaccg gccacaccga tcggcccccg aaacatcggg tccaacccgc gggttgactg
     2101 cgcattcgat gcttcgccgg cgcttccctc ggcacagttg ttccacgctg gagcaggcgt
     2161 ggagcaggcg agggacgttt gatgccgctg ctttgcgtgc tcggcctgcc gttgaacctg
     2221 gtggcggtgg cggcagcagc tgcggcgctc tctcgcgcct gtgccgccgc cgcgccgagc
     2281 gcgacaagcg cgcgggtcat tcgaccggca cgctcgcccg cggcgcgccg tcgctgcggg
     2341 ccgtccgatc acatgctcgt caagccgatt cgctcattcg accgtgacgg actttgcaag
     2401 gtttctcggc ttgtcgacat cggtgccgcg tgcgcacgcg gtgtggtacg cgagcagctg
     2461 cagcggcacc acgtgcagga tcggcgacag tgcgccgtag tgctcgggca tgcggatcac
     2521 gtgcaggcct tcgctggaga cgatgcgcgt gtcggcatct gcgaacacga acagttcgcc
     2581 gccgcgcgcg cgcacttcct gcaggttgct cttgagtttt tcgagcagcg cgtcgttcgg
     2641 cgcgaccgtg acgaccggca tctcggcggt gacgagcgcg agcggcccat gcttgagctc
     2701 gccggccggg taggcctcgg cgtggatgta gctgatctcc ttcagcttca gcgcgccttc
     2761 gagcgcgatc gggtaatgca ggccgcggcc gaggaacagc gcgttctcct tgcgcgcgaa
     2821 ttcctgtgac cacgcgatca cctgcggctc gagcgcgagc acgccctgca ccgccagcgg
     2881 caggtggcgc agcgccgtca ggtggccggc ttcctgcgcg tcggtgagca ggccgcgcgt
     2941 ctgcgccagc gccaaggtca gcaggaacag cccgacgagt tgcgtgctga aggccttggt
     3001 cgacgcgacg ccgatctcga cgccggcgcg cgtgatgtag gcgaagcggc actcgcgcac
     3061 catcgcgctg gtcgcgacgt tgcagatcgt cagcgtgtgc gccatgccca ggccgcgcgc
     3121 gtgcttcagc gcggcgagcg tgtcggcggt ctcgccgctt tgcgtgatcg tgacgacgag
     3181 ggagcgcgga ttcggcacgc tgtcgcggta gcggtactcg ctcgcgacct cgacgctggt
     3241 cgggatcttc gcgatgctct cgagccagta cttggcgacc gagccggcgt agtagctggt
     3301 gccacaggcc aggatcagca ccgagtcgac gtccttgaac acgcggtgcg cgccgtcgcc
     3361 gaagagttcg ggcgtgatgc cgggcaccgc gtcgagcgtg tcggcgatcg ccttcggctg
     3421 ctcgaagatc tccttctgca tgtagtggcg gtaggggccg agctcggccg cgccactgtg
     3481 cgcgatcacg gtgcgcagct cgcgctgcac cggccgataa cagccattcg cgtcgcgctg
     3541 gctgatccag tgcttgccga gttgcaggtc gacgacgtcg ccctcttcga ggtagacgat
     3601 ctggtcggtc acgccggcca gcgccatcgc gtcggacgcg agaaaggtct cgccggcacg
     3661 ctcgccgcgg cccacgccga gcaccagcgg cgagccctcg cgcgcgccga ccacgcggtg
     3721 cggctcgtcg cggcagaaca ccgcgatcgc gaacgcgccg cgcagccgca cgaccgcgcg
     3781 ctgcaccgcg tcgaacaggt cgccgtcgta g
//
LOCUS       DATMJB010000058         4306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_287491, whole genome shotgun sequence.
ACCESSION   DATMJB010000058 DATMJB010000000
VERSION     DATMJB010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4306
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287491"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1650)
                     /locus_tag="VNU48_01335"
     CDS             complement(100..1650)
                     /locus_tag="VNU48_01335"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HWI09951.1"
                     /translation="MAAAADGAHDTPVRPAEVALLASVFVVAACGLVYELAAGTLASY
                     LLGDSVLQFSTVIGTYLFAMGLGSYLSRFFDRQLVAHFLRIELLVGLVGGLMPLMLFA
                     LQSLASPSFRFALYAMVLAVGVLVGLEIPLVMRILKRQPHGGFKNLVSQVLTFDYLGA
                     LAVAIAFPLLLVPQLGLIRTGALFGLMNAAVAVWALLLFRGELRRLEAHALACALTVA
                     ALIAAFAAADHVTTWAEDRFYADHVLFSESTPYQRVVVTASSQGVRLFLNGNLQFHSR
                     DEYRYHEALVHPAMAGQGAPKNVLVLGGGDGLALREILKYASVEHVTLVELDPRMTRL
                     FSTQPLLRELNADALRSSKLTIVNADAFAWLQQATDSYDVIVIDFPDPTNFAIGKLYT
                     LSFYRLVERHLAASGYAVVQTTSPLIARKSFWTVATTLEAAGLATTPYHAHVPSFGEW
                     GFILASRRPYRMPTTLPPGLRFLSPADLPTLFNFPLDMTRVPTDVNRLSNQVLVQTFE
                     EEWGKVHQ"
     gene            complement(1650..2084)
                     /locus_tag="VNU48_01340"
     CDS             complement(1650..2084)
                     /locus_tag="VNU48_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HWI09952.1"
                     /translation="MIAVDTNVLVYAHRAESTFHDPARRAIAALATSSASWAIPWPCV
                     HEFYGVVTNARAFRPASTTAQASLQLSSWFASPSLVLLHEGANHWSTLHALLVAGRVI
                     GGQMHDARIAALCIEHGVDEFWTADRDFTRFPTLPVRNPLAV"
     gene            complement(2081..2329)
                     /locus_tag="VNU48_01345"
     CDS             complement(2081..2329)
                     /locus_tag="VNU48_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09953.1"
                     /translation="MKTTLDIQTALLDEVRKIAARDGETLRSLVEQGLRKVVAERSAK
                     ARPFRLRDASVGTPGAPSDYESLSWEAKRALMYEGRGG"
     gene            complement(2378..2602)
                     /locus_tag="VNU48_01350"
     CDS             complement(2378..2602)
                     /locus_tag="VNU48_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HWI09954.1"
                     /translation="MMGIEWLKPGVVAGSILYALIGVAIFWICFVLVDKLTPYRLWDE
                     IVEKKNVALAIVVGSMCIAIGMIVAAAVHG"
     gene            complement(2652..4250)
                     /locus_tag="VNU48_01355"
     CDS             complement(2652..4250)
                     /locus_tag="VNU48_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HWI09955.1"
                     /translation="MATSPQRAYRAACPNCGAPVEFRSAASAFAVCSFCKSTVVREGD
                     ALRKIGESAALFDDHSPLQLGAAGKYQGAAFTLVGRLQYRYAEGTWNEWHALFDSGKS
                     GWLSEDNGRYVIAFDAPLEGAAPSAASLRAGAPVVLAGQPWTVASVASATLIAAQGEL
                     PHPPTLQQGFVVSDLRSTRGEVGTLDYGDPATPRWSIGRSVAIGELAMTGLADAAEKT
                     LKGRSVECPSCGAALEVKLATTQSIVCHQCHAVVDVSQGVGGDLQHYAQVNGTEPLIP
                     LGSVGTLALGARQALPWQVVGYAERCEVPASDEDERSFWREYLLYHRSAGFAFIVDAQ
                     DGWSWTAPITGVPSGSGAVVSYQGATYRKLYDYTGMVTYVLGEFYWQVTQGQRTFNTD
                     YQGSGAASARRLNREQTGSGDTQEVVWSAGETLAADAVLKAFRLAPDQRAALQRDALP
                     TALGGGSLLAKLFFWAFVVIVVLMLWRCGSGGGSQNCNDVRNTFGAASSEYQNCLDSN
                     RSGGGYYGGTGGAFGGFSTGGGHK"
BASE COUNT          696 a         1533 c         1483 g          594 t
ORIGIN      
        1 caccgaccca gccgccgcca tagcgcggcg cgtcggcgcg cgtgcagccc gcgagcggcg
       61 ccagcgcggc gccggccagc aacgtgcgcc ggcgcaggtt cactggtgca ccttgcccca
      121 ttcttcctcg aaggtctgca ccagcacctg gttcgacagc cggttcacgt cggtcggcac
      181 gcgcgtcatg tcgagcggga agttgaacag cgtcggcagg tcggccgggc tcaggaagcg
      241 cagccccggc ggcaaggtcg tcggcatccg gtacggccgg cggctcgcga ggatgaagcc
      301 ccattcgccg aagctcggca cgtgcgcgtg gtacggggtg gtcgcgaggc ccgcggcttc
      361 gagcgtggtc gccacggtcc agaagctctt gcgtgcgatc agcggcgagg tcgtctgcac
      421 gaccgcgtag ccgctggccg cgaggtgccg ctcgacgagg cggtagaagc tcagcgtgta
      481 gagcttgccg atcgcgaagt tggtcgggtc gggaaagtcg atcacgatca cgtcgtagga
      541 gtcggtcgct tgctgcagcc acgcgaacgc gtcggcgttg acgatcgtca gcttcgacga
      601 acgcagcgca tcggcattca gctcgcgcag cagcggctgg gtcgagaaca gccgcgtcat
      661 gcgcgggtcg agctcgacca gcgtgacgtg ctcgactgat gcgtacttca ggatctcgcg
      721 cagcgccaga ccgtcgccgc cgccgagcac gagcacgttc ttcggcgcgc cctgcccggc
      781 catcgccggg tgcaccagcg cctcgtggta gcggtactcg tcgcgcgagt ggaactgcag
      841 gttgccgttc aggaacagcc gcacgccctg gctgctggcg gtgacgacga cgcgctgata
      901 cggcgtcgac tcgctgaaca gcacgtgatc ggcatagaag cgatcctcgg cccaggtggt
      961 gacgtgatcg gccgccgcga acgccgcgat cagcgcggcg accgtcagcg cgcaggccag
     1021 cgcatgcgcc tcgaggcgcc gcagctcgcc gcgaaacagc agcagcgccc agaccgcgac
     1081 cgccgcgttc atcagcccga acagcgcgcc ggtgcggatc agcccgagct gcggcaccag
     1141 cagcagcggg aacgcgatcg cgaccgcgag cgcaccgagg tagtcgaagg tcagcacctg
     1201 cgacaccagg ttcttgaagc caccgtgcgg ctggcgcttc aggatgcgca tcaccagcgg
     1261 gatctccagc ccgacgagca cgcccaccgc cagcaccatc gcatacagcg cgaagcggaa
     1321 cgacgggctc gcgagcgact gcagcgcgaa cagcatcagc ggcatcagcc cgccgaccag
     1381 cccgaccagc agctcgatgc gcaggaagtg cgcgaccagc tggcgatcga agaagcgcga
     1441 caggtacgag cccaggccca tcgcgaacag gtaggtgccg atcacggtcg agaactgcag
     1501 caccgagtcg cccagcaggt agctcgccag cgtgccggcc gcgagctcgt agaccagccc
     1561 gcaggccgcg acgacgaaga ccgacgcgag cagcgcgacc tcggccggcc gcaccggcgt
     1621 gtcgtgcgcc ccgtcggcag cggccgccat cagaccgcca gcgggttgcg caccggcagc
     1681 gtcgggaagc gggtgaaatc gcggtcggcg gtccagaact cgtcgacgcc atgctcgatg
     1741 cagagggcgg cgatgcgcgc gtcgtgcatc tgcccgccga tcacccggcc ggccaccagc
     1801 agcgcatgca gggtcgacca gtggttcgcg ccctcgtgca acagcaccag cgaaggcgac
     1861 gcgaaccagc tcgacagttg caggctggcc tgggccgtcg tcgatgcagg ccggaacgcg
     1921 cgggcattcg tcacgacgcc atagaactcg tgcacgcagg gccacgggat cgcccagctg
     1981 gcgctcgacg tggcgagcgc tgcgatcgcg cggcgggccg ggtcgtggaa cgtcgactcc
     2041 gcgcggtggg cgtagacgag cacgttcgtg tcgacggcga tcacccgccg cggccctcgt
     2101 acatcagggc gcgcttggct tcccaggaaa ggctttcgta gtcggacggc gcgccgggcg
     2161 tcccgaccga cgcgtcgcgc aacctgaacg gccgcgcctt ggcgctgcgc tcggccacca
     2221 ccttgcgcag gccctgctcg accagcgagc gcagggtctc gccgtcgcgt gcggcgattt
     2281 tccgcacctc gtcgagcagc gcggtctgga tgtccagtgt cgtcttcatg ggcgcctccg
     2341 atatgggcac ccatatcgta gcaccctgca acccacatca cccgtgcacg gcggccgcga
     2401 cgatcatgcc gatcgcgatg cacatcgagc cgacgacgat cgccagcgcg acgttcttct
     2461 tctcgacgat ctcgtcccac agccggtagg gcgtgagctt gtcgacgagc acgaagcaga
     2521 tccagaagat cgccacgccg atcagcgcgt acaagatcga tccggcaacg acgccgggct
     2581 tgagccactc gattcccatc atcgcgaacc tccagtcgaa ccgttgaaca tgcgaagccc
     2641 ggcgcgcgtc atcacttgtg cccgccgccg gtagagaagc cgccgaacgc gccgccggta
     2701 ccgccgtagt agccgccgcc gctgcgattg ctgtcgaggc aattctggta ctcgctggag
     2761 gcggcgccga aggtgttgcg cacgtcgttg cagttctgcg agcccccgcc gctgccgcag
     2821 cgccacagca tcagcacgac gatcacgacg aaggcccaga agaagagctt cgccagcagc
     2881 gagccgccgc cgagcgcggt cggcagcgcg tcgcgctgca gggccgcgcg ctggtcgggc
     2941 gcgaggcgaa aggccttcag cacggcgtcg gccgcgaggg tctcgccggc cgaccagacg
     3001 acctcctgcg tgtcgccgga gccggtctgc tcgcggttca ggcgccgcgc cgacgccgcg
     3061 ccgctgccct ggtagtcggt gttgaacgtg cgctggccct gcgtcacctg ccagtagaac
     3121 tcgccgagca cgtaggtcac catgccggtg tagtcgtaga gcttgcggta ggtcgcgccc
     3181 tggtagctga cgaccgcgcc gctgcccgag ggcacgccgg tgatgggcgc ggtccagctc
     3241 cagccgtcct gcgcatcgac gatgaacgcg aagcccgcgc tgcggtggta cagcaggtac
     3301 tcgcgccaga agctgcgctc gtcctcgtcg ctcgccggca cctcgcagcg ctcggcgtag
     3361 ccgacgacct gccacggcag cgcctgtcgc gcgccgagcg ccagcgtgcc gacgctgccc
     3421 agcgggatca gcggctccgt gccgttgacc tgcgcgtagt gctgcaaatc gccgccgacg
     3481 ccttgcgaca cgtcgacgac cgcatggcat tgatggcaga cgatcgactg cgtggtcgcg
     3541 agcttgacct cgagtgcggc gccgcagctc gggcattcga cgctgcggcc cttcagcgtc
     3601 ttctccgcgg catcggcgag gccggtcatc gcgagttcgc cgatcgccac cgagcggccg
     3661 atggaccagc gcggcgtggc cgggtcgccg tagtccagcg tgccgacctc gccgcgcgtg
     3721 ctgcgcaggt ccgagacgac gaagccctgc tgcagggtcg gcgggtgcgg cagttcgccc
     3781 tgcgccgcga tcagcgtcgc ggacgcgacc gacgcgaccg tccacggctg cccggccagc
     3841 accaccggcg ccccggcgcg caggctcgcg gcgctcggcg cggcgccttc cagcggcgcg
     3901 tcgaacgcga tcacgtagcg gccgttgtcc tcgctcagcc agcccgactt gccgctgtcg
     3961 aacagcgcat gccactcgtt ccaagtgcct tcggcgtagc gatactgcag ccggccgacc
     4021 agcgtgaagg ccgcaccctg atacttgccg gcggcgccga gctgcagcgg cgagtgatcg
     4081 tcgaacagcg cggcactctc gccgatcttg cgcagcgcgt cgccctcgcg cacgaccgtg
     4141 ctcttgcaga agctgcagac ggcgaatgcc gacgccgccg atcggaactc gaccggcgcg
     4201 ccgcaattcg ggcacgcagc gcgataggcg cgttgcgggg aagtcgccac gcgtcgacta
     4261 gagcgggttc actgcgatca ggtgagcttc ttcagcagct cggcct
//
LOCUS       DATMJB010000059         7336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_228187, whole genome shotgun sequence.
ACCESSION   DATMJB010000059 DATMJB010000000
VERSION     DATMJB010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228187"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1208
                     /gene="glcE"
                     /locus_tag="VNU48_01360"
     CDS             78..1208
                     /gene="glcE"
                     /locus_tag="VNU48_01360"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HWI09956.1"
                     /translation="MSPNDPALQVLIDRVQSARAAGAQLCIRGGGTKDFYGEAPQGEA
                     LDTRVLEGTSSYEPTELVVTTRCGTALAELEALLATKGQCLPFEPPHFAYAPGAASLS
                     ARHRGATVGGMVAAGLSGPSRAAVGSVRDHVLGATMLNGKGEVLTFGGQVMKNVAGYD
                     VSRLLAGSLGTLGVILEVSLKVMPAAPATVTLRLEMDQPRALAQLNEWGGIAVPLNAS
                     AWWDGNLIVRLRGALAAVQAARVRLGGDVVEPEHASPFWEGLRDHTDEFFVAARAAIA
                     TGASLWRLSVPQTAPVLALPGELMIEWGGAQRWLCSAAPAAAVRDATARVGGHATLFA
                     ARDKAPGAFAPLRSPLDRIHRELKKSFDPAGVFNPGRLYPGL"
     gene            1213..2463
                     /gene="glcF"
                     /locus_tag="VNU48_01365"
     CDS             1213..2463
                     /gene="glcF"
                     /locus_tag="VNU48_01365"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HWI09957.1"
                     /translation="MQTHLAPQFKGTPDGDAAEAILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLMKQVLEGHAPTRATQLHLDRCLTCRNCESTCPSGVEYGHLIDIVRPIV
                     DDQVERPTREKATRWLLKEGLTSPLFAPAMKLGQALRPLVPAALQGKVPARPGARAHQ
                     WPTRTHARKVLMLMGCVQPAMMPNINSATARVLDAAGIQTVVADEAGCCGAIRTHLND
                     AEGGRDDMRRNIDAWWPLISGGPGALAGIEAIVMNASGCGVTVKDYGHALARDPAYAD
                     KAARISALTRDLSELLPDLVPLLKAKVRLRKLPTQLAFHPPCTLQHGQQLRGGVESHL
                     NALGFDVRVAPNESHLCCGSAGTYSVLQPELAQRLRDRKLANLAPLDPQIIVSANIGC
                     IQHLQTGTSTPVRHWIEVLDDALV"
     gene            2538..2918
                     /locus_tag="VNU48_01370"
     CDS             2538..2918
                     /locus_tag="VNU48_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09958.1"
                     /translation="MPNISNWFLRLAVLYLIAGIGLGLFMAASNDHSMKPVHAHLNLL
                     GWVTLTLFGLFYRAVPTAAATTLAKLHFWLYVPAHFLQMVLLAMFYRGVVAVEPALGA
                     VSMLVGVAVLLFATVVWRNTPTTA"
     gene            complement(2934..3647)
                     /locus_tag="VNU48_01375"
     CDS             complement(2934..3647)
                     /locus_tag="VNU48_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09959.1"
                     /translation="MLFRRREVWLPTMAGWLLIVAAALLLVGALAVRAYDLLAPQKPA
                     HGHGGEGARTLVVEGWLTRAELQQAAALLREGHYQRVLTTGGPIPAEIDAGGWQTFAQ
                     RAAELLRAEGVAGVPVIAVPAPASMQERSYLSAVMVRDWARRSGAPLVAIDLYSAGTH
                     ARRSWLLYRMALGDAVEVGVLAARPTDHDARRWWTNSEGVKTTIGETLSLTWTRCCFW
                     PGAPGSSAERLVVPVPARP"
     gene            complement(3662..4408)
                     /locus_tag="VNU48_01380"
     CDS             complement(3662..4408)
                     /locus_tag="VNU48_01380"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006398627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HWI09960.1"
                     /translation="MRLEGKVAIVTGGGSGFGEGIAKKFVAEGAKVLIADRDAAGGER
                     VARALGANARSVCVDVAKAADVRAMVDAAQDHFGALNVLVNNAGVSHLPQPLEEVGED
                     DFDRILAINVKAIYLAARVVVPRFKAQKNGVILNIASTAGVSPRPRLSWYNASKGWVI
                     TATRAMAVELAPFGVRVVALNPVAGETPLLKTFMGEDTPEMRAKFLATIPLGRFSTPE
                     DLGNAACFLCSDEASMITGVAMEVDGGRCI"
     gene            complement(4423..5898)
                     /locus_tag="VNU48_01385"
     CDS             complement(4423..5898)
                     /locus_tag="VNU48_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWI09961.1"
                     /translation="MPQDAVVPFAIPFDTEKLCIGGVWRAAADGRTLALDNPSDGRAL
                     ARIARGAAADIDAAVAAAQAALAGDWGRMSAAERGRVLGAIGRKVLDNVELLAMLEAL
                     DVGKPLKQGRADAIALARYLEFYGGAADKVHGETLPFQNGYTALTWREPHGVTGHIVP
                     WNYPMQIVGRSVGAALAMGNACVLKPAEEACLTVLAFARIAMEAGLPAGALNVVPGLG
                     DEAGAALAAHAGVQHLSFTGSVATGLAVQTAAAKNAVPVTLELGGKSPQIVFADADLD
                     AALPYLVNAGLQNAGQTCSAASRILVEQKVFDAVRERMAQRYRALRIGPAVDDLDMGP
                     VISKRQQGIVEHFLALGRDSGLRIAAQGSLPANLPTGGCYVRPTLFDAVPADHALAQQ
                     EIFGPVQVLIAFDGEDEALRIANGTAFGLVAGVWTRDGGRALRMARALKCGQVFVNNY
                     GAGGGVELPFGGVKHSGHGREKGFEALYGFSTLKTVAIHHG"
     gene            5951..6901
                     /locus_tag="VNU48_01390"
     CDS             5951..6901
                     /locus_tag="VNU48_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI09962.1"
                     /translation="MGIFLLKRFATFGATLLAASIVVFAVQGVLPGNAAQVMLGESAT
                     PEAVRSLSQKLGLDQPVAVRYGRWVRGLLTGDLGTSVAYDTPVGELIRERLAVTAPLA
                     LLAMALTAALALTLGIYAAAHHNELGDVGVMAASQVGIAIPNFWFAILLILVFAVKLQ
                     WFSAGGFPGWGDEPWLAFKALILPAVALAVVQAAILARITRSAVLDVLREDFVRTARA
                     KGLNRRQVLWRHVLRNAFVPVLTVMGLQFANLLTGTVVVENVFSLPGLGRLIFQAISN
                     RDLLVVQNVVMLLAAAVIVINFVVDVLYAVIDPRLKVHAA"
     gene            6898..>7336
                     /locus_tag="VNU48_01395"
     CDS             6898..>7336
                     /locus_tag="VNU48_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI09963.1"
                     /translation="MSFFERAVRHKSFFIGALLVALLIGTALLSFVWTPYSATELDIP
                     NKLQPPGAAHWLGTDSLGRDIVSLLIVGARNSIAVGLIAVGIGIGFGVALGLLAAARR
                     GWVEELVMRFSDFTFAFPALLSAIMLTAVYGPGLVTSIVAIGIF"
BASE COUNT         1021 a         2639 c         2572 g         1104 t
ORIGIN      
        1 acggcaagat gcatgtgcgg cgcgggcttt tgccgttcgc cgacattccg cgcttctgag
       61 cgcgcacgac gcaccgcatg agcccgaacg accccgcgct tcaagtcttg atcgatcgcg
      121 tccaatcggc ccgtgcggcc ggcgcgcagc tgtgcatccg cggcggcggc acgaaggact
      181 tctacggcga ggcgccgcag ggcgaggcgc tcgacacccg cgtgctcgaa ggcacctcga
      241 gctacgagcc gaccgagctc gtcgtcacga cgcgctgcgg caccgcgctg gccgagctcg
      301 aggcgctgct cgcgaccaag ggccagtgcc tgccgttcga gccgccgcac ttcgcgtacg
      361 cgccgggcgc cgcgtcactg agcgcacgcc accgcggcgc gaccgtcggc ggcatggtcg
      421 ccgccgggct gtcggggccg tcgcgcgcgg cggtcgggtc ggtgcgcgac cacgtgctcg
      481 gcgcgacgat gctcaacggc aagggcgagg tgctgacctt cggcggccag gtgatgaaga
      541 acgtcgccgg ctacgacgtc tcgcggctgc tcgccggctc actcggcacg ctgggcgtga
      601 tcctcgaggt ctcgctgaag gtgatgccgg ccgcgcccgc gaccgtgacg ttgcggctcg
      661 agatggacca gccgcgcgcg ctcgcgcagc tcaacgagtg gggtggcatt gcggtgccgc
      721 tgaacgccag cgcctggtgg gacggcaacc tgatcgtgcg cctgcgcggc gcgctcgccg
      781 cagtgcaggc ggcgcgcgtg cggctgggcg gcgacgtggt cgagcccgag cacgcgtcgc
      841 cgttctggga gggcttgcgc gaccacaccg acgagttctt cgtcgccgca cgcgccgcga
      901 tcgccaccgg cgcgtcgctg tggcgcctgt cggtgccgca gacggcgccg gtgctcgcgc
      961 tgccgggcga gctgatgatc gaatggggtg gcgcgcagcg ctggctgtgc agcgcggcgc
     1021 cggccgccgc ggtgcgcgac gcgacggcac gcgtcggcgg ccacgcgacg ctgttcgccg
     1081 cgcgcgacaa ggcgcccggc gcattcgcgc cgctgcgctc gccgctcgac cgcatccacc
     1141 gcgagctgaa gaagtcgttc gacccggcgg gcgtgttcaa ccccggccgg ctctacccgg
     1201 gcctttgaga cgatgcagac ccacctcgcc cctcagttca aaggcacgcc cgacggcgac
     1261 gcggccgagg cgatcctgcg caagtgcgtg cactgcggct tctgcaccgc gacctgcccg
     1321 acgtatcaac tgctcggcga cgagctcgac ggcccgcgcg ggcgcatcta cctgatgaag
     1381 caggtgctcg agggccatgc gccgacgcgc gcgacgcagc tgcacctgga ccgctgcctg
     1441 acctgccgca actgcgagag cacctgcccg tcgggtgtcg agtacggcca cctgatcgac
     1501 atcgtgcggc cgatcgtcga cgaccaggtc gagcggccga cgcgcgagaa ggcgacgcgc
     1561 tggctgctga aagagggcct gacctcgcct ctgttcgcgc cggcgatgaa gctcggccag
     1621 gcgctgcgcc cgctggtgcc ggcggcgctg cagggcaagg tgccggcgcg gcccggcgcg
     1681 cgcgcgcacc agtggccgac gcgcacccac gcgcgcaagg tgctgatgct gatgggctgc
     1741 gtgcagccgg cgatgatgcc caacatcaac agcgccaccg cgcgcgtgct cgacgccgcc
     1801 ggcatccaga ccgtcgtcgc cgacgaggcc ggctgctgcg gcgcgatccg cacgcacctg
     1861 aacgacgccg agggcgggcg cgacgacatg cgccgcaaca tcgacgcgtg gtggccgctg
     1921 atctcgggcg ggccgggggc gctcgccggc atcgaggcga tcgtgatgaa cgcgtcgggc
     1981 tgcggcgtga cggtgaagga ctacggccac gcgctcgccc gcgacccggc ctatgccgac
     2041 aaggccgcgc gcatcagcgc gctgacgcgc gacctgagcg agctgctgcc ggacctggtg
     2101 ccgctgctca aggccaaggt gcgactgcgc aagctgccga cccagctcgc gttccacccg
     2161 ccgtgcacct tgcagcacgg tcagcaactg cgcggcggcg tcgagagcca tctgaacgcg
     2221 ctcggcttcg acgtgcgcgt ggcgcccaac gagagccacc tgtgctgcgg ctcggccggc
     2281 acctactcgg tgctgcagcc cgagctcgcg cagcgcctgc gcgatcgcaa gctcgcgaac
     2341 ctcgcgccgc tcgatccgca gatcatcgtc tcggccaaca tcggctgcat ccagcatctg
     2401 cagaccggca cgagcacacc ggtgcggcac tggatcgagg tgctggacga cgcgctcgtc
     2461 tgagcctgag gcacttggag caggcaccga atccgcgtag catcgatacg ttcccttaca
     2521 aacacatcgg agttcatgtg cccaacatca gcaattggtt tctgcgcctg gccgtcctgt
     2581 acctgattgc cggcatcggc ctgggtctgt tcatggccgc cagcaacgat cactcgatga
     2641 agccggtgca cgcgcacctg aacctgctcg gctgggtgac gctgaccctg ttcggcctgt
     2701 tctaccgcgc ggtgccgacc gcggccgcga cgacgctggc caagctgcac ttctggctct
     2761 acgtgccggc gcacttcctg cagatggtgc tgctggcgat gttctaccgt ggggtcgtgg
     2821 cggtcgagcc ggcgctcggc gcggtgtcga tgcttgttgg cgtcgcggtt ctgctgttcg
     2881 ccaccgtcgt gtggcggaac acgccaacga cggcgtagcc gcgcgcgaag tcgctagggt
     2941 cgagcgggca ccggcacaac caggcgctcg gcggacgaac ccggcgcccc gggccagaag
     3001 caacaccggg tccaggtcag gctcagcgtc tcgccgatgg tcgtcttgac gccctcgctg
     3061 ttggtccacc agcggcgcgc gtcgtggtct gtcgggcgcg ccgcgagcac accgacctcg
     3121 accgcatcgc ccagcgccat ccggtagagc agccacgagc ggcgcgcatg cgtgccggcg
     3181 gaatagagat cgatcgccac gagcggtgcg cccgaacgcc gcgcccaatc gcgcaccatc
     3241 accgcgctca gatagctgcg ttcctgcatc gacgccggcg caggcacggc gatgaccggc
     3301 acgcccgcca cgccctcggc gcgcagcagt tcggcggcac gttgcgcgaa ggtctgccag
     3361 ccgccggcat cgatctccgc cgggatcggc ccaccggtcg tcagcacacg ctggtaatgt
     3421 ccctcgcgca gcagggccgc ggcctgttgc aactcggcgc gggtcaacca gccttcgacg
     3481 accagcgtgc gcgcaccctc gccgccatga ccgtgcgccg gcttttgcgg tgcgagcaga
     3541 tcgtaggcgc gcaccgccag cgcgccgacg agcagcagcg cggcggcgac gatcagcaac
     3601 cagcccgcca tcgttggcag ccagacctcg cgacggcgaa acagcacggc gccgcgcccg
     3661 ctcagatgca gcgcccgccg tcgacttcca tcgcgacgcc ggtgatcatc gaggcctcgt
     3721 cgctgcacag gaagcaggcg gcgttgccga ggtcctcggg cgtcgagaag cggccgagcg
     3781 ggatggttgc gaggaacttc gcgcgcattt cgggcgtgtc ctcgcccatg aaggtcttca
     3841 gcagcggcgt ctcgcccgcc accgggttca gcgcgaccac gcgcacgccg aaaggcgcga
     3901 gctcgaccgc catcgcgcgc gtcgcggtga tgacccagcc cttgctcgcg ttgtaccagc
     3961 tcaggcgcgg ccgcgggctg acgccggcgg tgctcgcgat gttcaggatc acgccattct
     4021 tctgcgcctt gaagcgcggc acgacgacgc gcgcggcgag atagatcgcc ttcacattga
     4081 tcgcgaggat gcggtcgaag tcgtcctcgc cgacctcttc cagcggctgc ggcaggtgcg
     4141 agacgccggc gttgttgacg agcacgttca gcgcgccgaa atgatcctgc gccgcgtcga
     4201 ccatcgcgcg cacatcggcc gccttcgcga catcgacgca gacgctgcgc gcgttcgcgc
     4261 ccagcgccct ggcgacgcgc tcgccgcccg ccgcgtcgcg gtcggcgatc agcaccttcg
     4321 cgccctcggc gacgaatttc ttcgcgatgc cctcgccgaa gccggaaccg ccgccggtga
     4381 cgatcgccac cttgccttcg agtctcatca gcgaactcgc cgtcatccgt gatgaatcgc
     4441 gaccgtcttc agggtcgaga acccgtacag cgcctcgaag cccttctcgc gcccgtggcc
     4501 cgaatgcttg acgccgccga acggcagctc gacgccgccg ccggcaccgt agttgttgac
     4561 gaacacctgg ccgcacttga gcgcgcgtgc catgcgcagc gcccgcccgc cgtcgcgcgt
     4621 ccagacgccg gcgaccaggc cgaacgcggt gccgttggcg atccgcagcg cctcgtcctc
     4681 gccgtcgaac gcgatcagca cctgcaccgg gccgaagatc tcctgctgcg cgagcgcgtg
     4741 gtcggcgggc accgcgtcga acagcgtcgg gcgcacgtag cagccaccgg tcggcagatt
     4801 cgccggcaag ctgccctgcg cggcgatgcg cagcccgctg tcgcggccga gcgcgagaaa
     4861 gtgttcgacg atgccctgct ggcgcttcga gatcaccggg cccatgtcga ggtcgtcgac
     4921 agcgggcccg atgcgcaatg cccggtagcg ctgcgccatg cgctcgcgca ccgcgtcgaa
     4981 caccttctgc tcgaccagga tgcgcgacgc cgccgaacag gtctgcccgg cgttctgcaa
     5041 cccggcgttg acgagatagg gcagcgcggc gtcgaggtcg gcatcggcga acacgatctg
     5101 cgggctcttg ccgccgagtt ccagtgtcac cggcaccgcg ttcttcgccg ccgcggtctg
     5161 gacagccaag ccggtggcca ccgagccggt gaacgacagg tgctgcacgc ctgcatgcgc
     5221 cgcaagcgcc gcgccggcct cgtcgcccag gccgggcacg acattcagcg caccggccgg
     5281 cagcccggct tccatcgcga tgcgcgcgaa cgcgagcacc gtcagacagg cttcctcggc
     5341 aggcttgagc acgcaggcgt tgcccatcgc gagcgccgcg ccgacgctgc ggccgacgat
     5401 ctgcatcgga tagttccagg gcacgatgtg cccggtcacg ccgtgcggct cgcgccaggt
     5461 cagcgcggtg tagccgttct ggaacggcag ggtttcgccg tgcaccttgt cggcggcgcc
     5521 gccgtagaac tcgaggtagc gcgcgagtgc gatcgcgtcg gcacggccct gcttcagcgg
     5581 cttgccgaca tcgagcgcct cgagcatcgc gagcagctcg acgttgtcga gcaccttgcg
     5641 cccgatcgca cccagcacgc ggccgcgttc ggcggcgctc atgcggcccc agtcaccggc
     5701 gagcgcggcc tgcgcggcgg cgaccgcggc gtcgatgtcg gccgcagcgc cgcgcgcgat
     5761 gcgggccagc gcgcggccgt ccgacgggtt gtcgagcgcc agcgtgcggc catcggcggc
     5821 ggcgcgccag acgccgccga tgcagagctt ctcggtgtcg aacgggatcg cgaacggaac
     5881 aacagcgtct tgcggcatcg cggcctccag tcgcccgggc gcgggcccgg ggatgcgcca
     5941 tgatattggc atgggcatct tcctcctgaa gcgcttcgcg accttcggcg cgaccctgct
     6001 cgccgcctcg atcgtcgtct tcgcggtgca gggcgtgctg cccggcaatg cggcgcaggt
     6061 gatgctgggc gagtcggcca cgcccgaggc ggtgcgctcg ctgtcgcaga agctcgggct
     6121 ggaccaaccc gtcgcggtgc gctatggccg ctgggtgcgc ggcctgttga ccggcgacct
     6181 cggcaccagc gtggcctacg acacgccggt gggcgagctg atccgcgagc gcctcgcggt
     6241 gaccgcgccg ctggcgctgc tggcgatggc gctgaccgcg gcgctcgcgc tcacgctggg
     6301 catctacgcg gcggcgcacc acaacgagct cggcgatgtc ggcgtgatgg cggcgagcca
     6361 ggtcggcatc gcgatcccga acttctggtt cgcgatcctg ctgatcctgg tgttcgcggt
     6421 gaagctgcag tggttctcgg ccggcggctt tccgggttgg ggcgacgagc cgtggctggc
     6481 cttcaaggcg ctgatcctgc cggccgtcgc gctcgcggtc gtgcaggcgg cgatcctggc
     6541 gcgcatcacg cgctcggcgg tgctcgacgt gctgcgcgag gatttcgtgc gcaccgcgcg
     6601 cgcgaaaggc ctgaaccggc gccaggtgct gtggcgccat gtgctgcgca acgccttcgt
     6661 gccggtgctc acggtgatgg ggctgcagtt cgcgaacctg ctgaccggca ccgtggtggt
     6721 cgagaacgtg ttctcgctgc ccggcctggg ccgactgatc ttccaggcga tctcgaaccg
     6781 cgatctgctg gtggtgcaga acgtcgtgat gctgctggcc gccgcggtga tcgtgatcaa
     6841 cttcgtcgtc gacgtgctgt acgcggtgat cgacccgcgg ctgaaggttc acgccgcatg
     6901 agcttcttcg agcgggccgt gcgccacaag agcttcttca tcggtgctct gctggtcgcg
     6961 ctgctgatcg gcacggcgct gctgtccttc gtctggacgc cgtattcggc caccgagctc
     7021 gacatcccga acaagctgca gccgccgggc gcggcgcact ggctcggcac cgacagcctg
     7081 ggccgcgaca tcgtctcgct gctgatcgtc ggcgcacgca actcgatcgc ggtcgggctg
     7141 atcgcggtcg gcatcggcat cgggttcggc gtcgcgctcg ggctgctcgc ggcggcacgg
     7201 cgcggctggg tcgaggaact ggtgatgcgg ttttcggact tcaccttcgc gttcccggcg
     7261 ctgctctcgg cgatcatgct gaccgcggtg tacgggcccg ggctcgtgac ctcgatcgtc
     7321 gcgatcggca tcttca
//
LOCUS       DATMJB010000060        10882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_227779, whole genome shotgun sequence.
ACCESSION   DATMJB010000060 DATMJB010000000
VERSION     DATMJB010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10882
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227779"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VNU48_01400"
     CDS             <1..904
                     /locus_tag="VNU48_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWI09964.1"
                     /translation="TPVMAVVTNIDADHMDTYGHDFAKLKSAFVEFIHRMPFYGAAIL
                     CADDANVRAIMPMISRPVVTYGFAEDAMVRAVDIEADAGTMRFTVQRRNGVRMPDLRV
                     TLNLPGDHNVLNALAAIAVATELELPDAPVIKGLAEFHGVGRRFQRYGEQPTGDGGTF
                     TLIDDYGHHPVEMAATLAAARGAFPGRRLVLAFQPHRYTRTRDCFEDFVKVIATADRV
                     LLAEVYAAGEAPIVAADGRALARALRVAGKVDPVFVDEIADMPQTIVEQVRDGDVVIA
                     MGAGSIGNVPAQVVELLGAKGATT"
     gene            901..1920
                     /locus_tag="VNU48_01405"
     CDS             901..1920
                     /locus_tag="VNU48_01405"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HWI09965.1"
                     /translation="MSVDVKALGKVAVLMGGSSAEREISLMSGKGVLAALRSRGVDAH
                     AFDPSERELVELERDGFDRCFITLHGRHGEDGSVQGALELLGIPYTGSGVLASAIAMD
                     KVMTKRVWIAEGLPTPRYLWLAPEDRGRARVRSVPDELGLPLIVKPPREGSSIGITKV
                     AGYSQMQGAVELAAKYDPDVLCEEFIEGEEVTCPVLGAGANAHALPVVKIVAPEGAYD
                     YQNKYFTDVVDYRVPSGLPLAEEREIQRLVVQAYRTLGCRGWGRADLMIRARDRKPFL
                     LEMNTSPGMTSHSLVPKSAAAAGLSYEALCVDILARAALDAAPRDPSALDAAPRDPSA
                     PDASH"
     gene            1926..2738
                     /locus_tag="VNU48_01410"
     CDS             1926..2738
                     /locus_tag="VNU48_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HWI09966.1"
                     /translation="MATRASAAPALPADIRLMNATAAVLGAIGVVALVVVAVLWAAHR
                     PVFAVKAIRIDGDLTHNSALTIRANAAPKLNGNFFTLDLANAQRAFQSVPWVRQAIVK
                     RVWPNRLAVQLEEHRPAALWGGAAGSDDAVSDKLVNNHGEVFEANLGDVEDEALPTLR
                     GPEGTSAHLLAMLGRLRPEFAAMDAHIDTLELSGRGSWRVELDTGAEVELGRGSDDEV
                     IARTRSFVATLPEVRNRYQQHPLLSADLRHNAGYAVRLEGVSTSADAAGKKK"
     gene            2752..3981
                     /gene="ftsA"
                     /locus_tag="VNU48_01415"
     CDS             2752..3981
                     /gene="ftsA"
                     /locus_tag="VNU48_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWI09967.1"
                     /translation="MAKEYKDLVVGLDIGTAKIMVVVAEVMADGELRIAGLGSAPTHG
                     LKRGVVVNIDATVQSIQQALKEAEMMADCKITRVYTGITGSHIRGQNSTGMVIVRDKE
                     VTPVDVARVVETAKAINIPNDQRLLLVEPQEFVIDGHEVKEPIGMSGGRLEVKVHIVT
                     GAQSAAENIVKCVRRCGLEVDQLVLNPSASSHSVLTEDERDLGVALVDIGAGTTDVAI
                     FTDGAIRHTAVIPIAGDLITSDIAMALRTPTKDAEEIKVEYGVAKQLLADPAEQLEVP
                     GLGDRAPRMLSRQALAGVIEPRIEEIYSLVHQVIRDSGYEELLSSGIVITGGAAVMPG
                     MVELGEDIFLKPVRKGVPTYHGALHDMVANPRSATVMGLLEEARFARTRGLRAAQQAG
                     SVKTLFGRAKDWFLGNF"
     gene            4157..5368
                     /gene="ftsZ"
                     /locus_tag="VNU48_01420"
     CDS             4157..5368
                     /gene="ftsZ"
                     /locus_tag="VNU48_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWI09968.1"
                     /translation="MAIEMIEEFNMGTQIRVIGVGGGGGNAVDHMIAQGVQGVQFVCA
                     NTDAQALNRSAAHELIQLGTTGLGAGAKPEAGKAAAEEAVDRIRQAIDGSHMLFITAG
                     MGGGTGTGAAPVIARVAKEMGILTVGVVTKPFEFEGNRRMKAADSGLAELEANVDSLI
                     VILNDKLLDVLGDDITQDQAFAHANDVLKNAVGGISDIIHMPGLVNVDFEDVKTVMSE
                     PGKAMMGTAQAGGPDRAIKAAEAAVACPLLEGIDLSGARGVLVLIAANRNTFKLAESR
                     NAMNTIRRYAAEDAHVIYGTAYDESLGDQLRVTVIATGLSPARRQQAPISVVHSMPAQ
                     RTGTHDLPILQSAVNNNAPHGIAPAHDYSALTTPSVWRNGRSAAAKVDALASNGMDEI
                     EIPAFLRKQAD"
     gene            5553..6698
                     /locus_tag="VNU48_01425"
     CDS             5553..6698
                     /locus_tag="VNU48_01425"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI09969.1"
                     /translation="MLIRRALQDDVRAALARSPAVALIGPRQVGKTTLARSLLGADSA
                     NYFDLEDPQVEAQLAAPMTALKDLRGLVVIDEVQHAPELFKVLRVLIDRPGDPARFLV
                     LGSASPALLRQTSESLAGRVEVIETGGFTLDETGADQGAVLWWRGGFPRSYTAADDAV
                     SRVWRREFIRTVIERDLPQLGIGAPAPAMHRFWSMLAHCHGQVWNAADPARSLGVNES
                     TVRRYLDWLTQAFLVRQLPPWHANLGKRQVKAPKVYVRDSGLLHELLGLESAAALSRH
                     PKSGASWEGFALDQVLRIAKPDAAYFWATHAGAELDLLMLKDGRRIGVEFKRSDAPNL
                     TASMRVAMVDLELEALYVVYPGTRRYLLAPQVEAVPLAALVAPEAPT"
     gene            6763..7698
                     /gene="lpxC"
                     /locus_tag="VNU48_01430"
     CDS             6763..7698
                     /gene="lpxC"
                     /locus_tag="VNU48_01430"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HWI09970.1"
                     /translation="MLAQRTLKSMTRAVGVGLHGGQRVELTLRPAPVDTGIVFRRVDL
                     AQPVDIRVNALWITDTRMATTLSAGGDPGAPKVKTIEHLLSACSGLGLDNLIVDINGE
                     EVPILDGSAASFVFLLQSAGIELQDAPKRFVRMLRAVEVREGAGPALKWARLEPFEGY
                     KLTFEIDFDHPAVDQTGQRVSFDMGSGRYKQEIARARTLGFTKDVDMMRARGLALGGS
                     MDNALVIDDYKVLNDGGLRYDDEIVKHKILDAIGDMLVIGHPMIGAYSAFRAGHDLNN
                     KLLRAVLADPASFDLVSFDDATRAPAGLAELAPAW"
     gene            7702..7887
                     /locus_tag="VNU48_01435"
     CDS             7702..7887
                     /locus_tag="VNU48_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09971.1"
                     /translation="MLLVRGLVVLLLVAGAAFLVAYMVTGQKRYQAAGVRIVKWTVIA
                     ALGFFAVLIVEQLATMR"
     gene            7898..8104
                     /locus_tag="VNU48_01440"
     CDS             7898..8104
                     /locus_tag="VNU48_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09972.1"
                     /translation="MTIFRWIIGVLAALLATGALLSLALFLAFDIEVWLKRARTLRHG
                     VYMALLLWFNVEIWGRVLGALIHW"
     gene            complement(8222..8806)
                     /locus_tag="VNU48_01445"
     CDS             complement(8222..8806)
                     /locus_tag="VNU48_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HWI09973.1"
                     /translation="MPSAVTHLLYLHGFRSSPQSTKARLTAAWLQQHRPDVHWWCPQL
                     PASPRDALQLVMDGVADWPAAGIGVIGSSLGGFYATAVAERLGCRAVLLNPAIDPARD
                     LQTYIGETTAWHSDERFFFRAEFIAELRAIAPATLTRLDRYFAVIAKGDELLSWVEMS
                     ARYRGAAIRLLEGGDHALSDFDTHLPEVMAFLGI"
     gene            complement(8816..10234)
                     /gene="mpl"
                     /locus_tag="VNU48_01450"
     CDS             complement(8816..10234)
                     /gene="mpl"
                     /locus_tag="VNU48_01450"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HWI09974.1"
                     /translation="MHIHILGICGTFMGGLAALAREGGHTVTGCDTNVYPPMSDQLRA
                     LGIELVEGYGAEQLAFRPDLFVIGNVIVRGNPLMEAILDAGAPYTSGPQWLADHVLQG
                     RHVLAVAGTHGKTTTTSMLAWILEQAGLAPGFLVGGVPLNFGVSARLGSASPGARLGQ
                     AEPGSAFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFDDLAAIERQFHH
                     LVRTVPAHGRLVVNAREDSLKRVLAMGCWSEVTRFGARKEEPGALRARGEPHAFDVLR
                     GSLKVARVEWPLLGEHNQLNALAAIAAAEHVGVAPDVAGRALASFENVRRRLELRGEA
                     GGVKVYDDFAHHPTAIRTTVNGLRRRVDHEKTAPGAPRQRILAVFEPRSNTMKLGAMK
                     AQLPWSLEEADLAFCHSGGLGWDAAQALAPMGAQAVVCDTIDELVKRVVAAARPGDHV
                     LCMSNGAFSGVHAKLLNALAKR"
     gene            complement(10278..>10882)
                     /locus_tag="VNU48_01455"
     CDS             complement(10278..>10882)
                     /locus_tag="VNU48_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWI09975.1"
                     /translation="AAATLGVPLGDADYLTLIGLNHVDSGARLLRLFGGDAARLAAAG
                     DLVESQLNERFGDRPFDTKPGALALLRRLHAARVPCAVASSTHRGEVQRRLGKAGLLD
                     FFAAVCGGDEVRCGKPAPDLYALAVARLGAAAATALAFEDSGHGVQAALEAGLAVVAV
                     PDLLAPEPAWQARCLAVLPSLEAVGAHGAEWFGFGRVGAA"
BASE COUNT         1698 a         3749 c         3871 g         1564 t
ORIGIN      
        1 gacgccggtg atggcggtgg tcacgaacat cgacgctgac cacatggaca cctacggcca
       61 cgacttcgcg aagctgaagt ccgcgttcgt cgagttcatc caccgcatgc cgttctacgg
      121 cgcagcgatc ctgtgcgccg acgacgcgaa cgtgcgcgcc atcatgccga tgatctcgcg
      181 gcccgtcgtg acctacggct tcgccgagga cgcgatggtg cgcgcggtcg acatcgaggc
      241 cgacgccggc acgatgcgct tcacggtgca gcgccgcaac ggcgtgcgca tgcccgacct
      301 gcgcgtcacg ctcaacctgc cgggcgacca caacgtgctg aacgcgctcg ccgcgatcgc
      361 ggtcgcgacc gagctcgagc tgcccgatgc gccggtgatc aagggattgg ccgagttcca
      421 cggcgtcggc cggcgcttcc agcgctacgg cgagcagccg accggcgacg gcggcacgtt
      481 cacgctgatc gacgactacg gccaccatcc ggtcgagatg gcggccacgc tcgccgccgc
      541 gcgcggcgcg ttcccgggcc ggcgcctggt gctcgcgttc cagccgcacc gctacacgcg
      601 aacgcgcgac tgcttcgagg acttcgtcaa ggtcatcgcc accgccgacc gggtgctgct
      661 ggccgaggtc tacgccgccg gcgaggcgcc gatcgtcgcc gccgacggcc gcgcgctggc
      721 gcgtgcactg cgcgtggccg gcaaggtcga tccggtgttc gtcgacgaga tcgccgacat
      781 gccgcagacc atcgtcgagc aggtgcgcga cggcgacgtc gtgatcgcga tgggcgccgg
      841 ttcgatcggc aacgtgccgg cgcaggtggt ggaactgttg ggcgcgaagg gggcgacaac
      901 atgagcgtcg atgtgaaggc cctcggcaag gtcgcggtgc tgatgggcgg cagttcggcc
      961 gagcgcgaga tctcgctgat gtcgggcaag ggcgtgctcg cggcgctgcg ctcgcgcggc
     1021 gtcgatgcgc acgcgttcga cccgagcgag cgcgagctgg tcgagctcga gcgcgacggc
     1081 ttcgaccgct gcttcatcac gctgcacggc cgccacggcg aagacggcag cgtgcagggt
     1141 gcgctcgaac tgctgggcat cccgtacacc ggctcgggcg tgctcgcgtc ggcgatcgcg
     1201 atggacaagg tcatgaccaa gcgtgtctgg atcgccgaag gcctgccgac gccgcgctac
     1261 ctctggctcg cgcccgagga ccgcggccgc gcgcgcgtgc gcagcgtgcc cgacgaattg
     1321 ggcctgccgc tgatcgtcaa gccgccgcgc gagggctcgt cgatcggcat caccaaggtc
     1381 gcgggctatt cgcagatgca aggcgccgtc gaactcgccg cgaagtacga ccccgacgtg
     1441 ctgtgcgagg agttcatcga gggcgaggag gtgacctgcc cggtgctcgg cgcgggcgcg
     1501 aatgcgcacg cgctgccggt ggtcaagatc gtcgcgcccg aaggcgccta cgactaccag
     1561 aacaagtact tcaccgatgt cgtcgactac cgcgtgccca gcggcctgcc gctggccgag
     1621 gaacgcgaga tccagcgcct cgtcgtgcag gcctaccgca cgctcggctg ccgcggctgg
     1681 ggtcgcgcgg acctgatgat ccgcgcgcgc gaccgcaagc ccttcctgct cgagatgaac
     1741 acctcgccgg gcatgacctc gcattcgctg gtgccgaagt cggccgccgc cgcgggcttg
     1801 agctacgagg cgctgtgcgt cgacatcctc gcccgcgcgg cgctcgacgc cgcgccgcgt
     1861 gacccttcgg cgctcgacgc cgcgccgcgt gatccttcgg cgccggacgc aagtcactga
     1921 acacgatggc cacccgcgcc tccgccgccc ctgcgctgcc ggccgacatc cggctgatga
     1981 acgcgaccgc cgccgtgctc ggcgcgatcg gcgtggtggc actcgtcgtc gtcgccgtgc
     2041 tgtgggccgc gcaccggccg gtgttcgcgg tgaaggcgat ccgcatcgac ggcgacctca
     2101 cgcacaacag cgcgctgacg atccgcgcca atgcggcgcc caagctcaac ggcaacttct
     2161 tcacgctgga cctggcgaac gcgcaacgcg cgttccagtc ggtgccgtgg gtgcgccagg
     2221 cgatcgtcaa gcgcgtctgg ccgaaccggc tggcggtgca gctcgaagag caccggcccg
     2281 cggcgctgtg gggcggcgcg gccggcagcg acgatgcagt ctccgacaag ctggtcaaca
     2341 accacggcga agtgttcgag gcgaatctcg gcgatgtcga ggacgaggcg ctgccgacct
     2401 tgcgcgggcc cgagggcacg tcggcccacc tgctcgcgat gctcggccgg ctgcgccccg
     2461 agtttgcggc gatggacgcg cacatcgaca cgctcgaact gtcgggccgc ggctcgtggc
     2521 gtgtcgagct cgacaccggt gccgaggtcg agctcgggcg cggcagcgac gacgaggtga
     2581 tcgcgcgcac gcgcagcttc gtcgcgacgc tgcccgaggt gcgcaaccgc taccagcagc
     2641 acccgctgct gtcggccgac ctgcgtcaca acgccggtta cgcggtgcgc ctggaaggcg
     2701 tgtcgacctc ggcagacgcc gccggcaaga agaaataggg ggaacagaag aatggccaag
     2761 gaatacaagg acctcgtcgt cggcctggac atcggcaccg ccaagatcat ggtggtggtg
     2821 gccgaggtca tggcggacgg cgagctgcgc atcgcgggcc tgggcagcgc gcccacgcac
     2881 ggcctgaagc gcggcgtggt tgtcaacatc gatgcgacgg tgcagagcat ccagcaggcc
     2941 ctgaaagagg ccgagatgat ggccgactgc aagatcacgc gcgtctacac cggcatcacc
     3001 ggcagccaca tccgcggcca gaactcgacc ggcatggtga tcgtgcgcga caaggaagtg
     3061 acgcctgtcg acgtcgctcg cgtcgtcgaa accgcgaagg cgatcaacat cccgaacgac
     3121 cagcggctgc tgctggtcga gccgcaggag ttcgtgatcg acggtcacga ggtcaaggag
     3181 ccgatcggca tgagcggcgg gcgcctggaa gtgaaagtgc acatcgtcac cggcgcgcag
     3241 agcgcggccg agaacatcgt caagtgcgtg cgccgctgcg ggctcgaagt ggatcagctg
     3301 gtgctgaacc cgagcgcgtc gagccactcg gtgctgaccg aggacgagcg cgacctcggc
     3361 gtcgcgctgg tcgacatcgg tgccggcacg accgacgtgg cgatcttcac cgacggtgcg
     3421 atccgccaca cggcggtgat tccgatcgcc ggcgacctga tcaccagcga catcgcgatg
     3481 gcgctgcgca cgccgaccaa ggacgccgag gagatcaagg tcgagtacgg cgtcgcgaag
     3541 cagctgctgg ccgacccggc cgagcagctc gaggtgccgg ggctgggcga ccgcgcgccg
     3601 cgcatgctga gccgccaggc gctggccggc gtgatcgagc cgcgcatcga ggagatctat
     3661 tcgctggtgc accaggtgat ccgcgacagc ggctacgagg agctgctctc gagcggcatc
     3721 gtgatcaccg gcggggcggc ggtgatgccg ggcatggtcg agctcggcga agacatcttt
     3781 ctgaagccgg tgcgcaaggg cgtgccgacc taccacggcg cgctgcacga catggtcgcg
     3841 aacccgcgct ctgccaccgt gatggggctg ctcgaagagg cgcgcttcgc gcgcacgcgc
     3901 ggcctgcggg cggcgcagca ggcggggtcg gtcaagaccc tgttcggccg cgccaaggac
     3961 tggttcctcg gcaacttttg aagaggcgga aaaacgatga agcacaccca ctcattgctg
     4021 cacgttcatt ccgcccacgg gctggcgccg ccgcgtgccg gacccgaacg acgacgacaa
     4081 caacgattca tgtggtgctg aacaagcact gattttccga agacacggca actgcactta
     4141 aggactggag caagaaatgg ccatcgaaat gatcgaagaa ttcaacatgg gcacgcagat
     4201 cagggtgatc ggcgtcggcg gcggcggcgg caacgccgtc gaccacatga tcgcgcaggg
     4261 tgtgcaaggc gttcagttcg tctgcgccaa caccgacgcg caggcgctga accgctcggc
     4321 cgcgcacgag ctgatccagc tcggcacgac cggcctgggc gcgggcgcca agcccgaggc
     4381 cggcaaggcc gcggccgagg aggcggtgga ccgcatccgc caggccatcg acggctcgca
     4441 catgctgttc atcaccgccg gcatgggcgg cggcaccggc accggcgcgg cgccggtgat
     4501 cgcgcgcgtc gcgaaggaaa tgggcatcct gacggtgggc gtcgtgacca agccgttcga
     4561 gttcgagggc aaccgccgca tgaaggcggc cgactcgggc ttggccgaac tcgaggcgaa
     4621 cgtcgactcg ctgatcgtga tcctgaacga caagctgctc gacgtgctcg gcgacgacat
     4681 cacgcaggat caggccttcg cgcacgccaa cgacgtgctg aagaatgcgg tgggcggcat
     4741 cagcgacatc atccacatgc cgggcctcgt gaacgtcgac ttcgaggacg tgaagacggt
     4801 gatgagcgag cccggcaagg cgatgatggg cacggcgcag gccggcggcc cggaccgcgc
     4861 gatcaaggcg gccgaggcgg cggtggcgtg cccgctgctc gagggcatcg acctgtcggg
     4921 cgcgcgcggc gtgctggtgc tgatcgcggc gaaccgcaac accttcaagc tggccgagag
     4981 ccgcaacgcg atgaacacga tccgtcgcta tgccgccgag gacgcgcacg tgatctacgg
     5041 caccgcctac gacgaaagcc tgggcgacca gctgcgcgtg accgtgatcg ccaccggcct
     5101 gagcccggcg cggcgccagc aggcaccgat cagcgtggtc cacagcatgc cggcgcagcg
     5161 caccggcacg cacgacctgc cgatcctgca gagcgcggtg aacaacaacg cgccgcacgg
     5221 catcgcaccg gcgcacgact acagcgcgct gacgacgccc agcgtgtggc gcaacggccg
     5281 cagcgcggcg gccaaggtcg atgcgctggc gagcaacggg atggacgaga tcgagatccc
     5341 ggcgttcctt cgcaagcaag ccgattgacg ctgcccgaag ctactgcgca ggccgatctg
     5401 cgtccggagg tgctcgccgt acgcaagtac ggctgcgctc ctgcgaacgc acctcggcct
     5461 tgctcgctac gcttctgaca gcatcaatct cgtgcgacgt tgaacattga aattcccgca
     5521 ttcaatgcgg gaatttcaag gtacgatttc tgatgttgat ccgccgagcc ctgcaagacg
     5581 acgtgcgcgc ggcgctggcg cgcagcccgg cggtggcgct gatcggcccg cgtcaggtcg
     5641 gcaagaccac gcttgcccgt tccctgctcg gggccgactc ggccaactac ttcgacctgg
     5701 aagacccgca ggtcgaagcc cagctcgccg cgccgatgac ggcgctgaag gacctgcgcg
     5761 gcctggtcgt gatcgacgag gtgcagcatg cgcccgaact gttcaaggtg ctgcgcgtgc
     5821 tgatcgaccg gccgggcgat ccggcgcgct tcctggtgct gggcagtgca tcgccggcac
     5881 tgttgcgcca gaccagcgag tcgctcgccg gccgcgtcga ggtgatcgag accggcggat
     5941 tcacgctcga cgagaccggt gccgaccaag gcgccgtgct gtggtggcgg ggtggcttcc
     6001 cgcgttcgta caccgccgcg gacgatgcgg tcagccgggt ctggcggcgt gaattcatcc
     6061 gcaccgtcat cgagcgcgac ttgccgcaac tcggcatcgg cgcgcccgcg ccggcgatgc
     6121 accgcttctg gtcgatgctg gcccactgcc acgggcaggt ctggaacgcg gcggatccgg
     6181 cgcggtcgct cggcgtcaac gagagcacgg tgcgacgcta tctggactgg ttgacccagg
     6241 cctttctggt gcgccagttg ccgccctggc acgcgaatct gggcaagcgt caggtcaagg
     6301 cgccaaaggt ctacgtgcgc gattcggggc tcttgcacga gttgctcggt ctcgagagcg
     6361 ccgcggcgct gtcccggcac ccgaagagcg gtgcctcctg ggaaggcttc gccctcgacc
     6421 aggtgctgcg catcgcgaag cccgacgccg cctacttctg ggccacccac gccggcgccg
     6481 aactcgacct gctgatgctg aaggacggcc ggcgcatcgg cgtcgagttc aaacggagcg
     6541 acgcgccgaa cctcacagcg tcgatgcggg tcgcgatggt cgatctcgag ctcgaggcgc
     6601 tgtacgtcgt ctacccgggc acgcgtcgat acctgttggc gccgcaagtc gaggcggtcc
     6661 cgctcgcggc gctcgttgca ccggaggccc cgacgtgacg gtgctgcgcg catcgggccg
     6721 agcggcgacc gcgcgccgcg ttttccatga caatcgccat cgatgctcgc ccaacgcacc
     6781 ctcaaatcga tgacccgcgc ggtcggcgtc ggcctgcacg gcggccagcg cgtcgagctg
     6841 acgctgcggc cggcaccggt cgacaccggc atcgtgttcc ggcgcgtcga cctggcgcag
     6901 ccggtggaca tccgcgtcaa cgcgctgtgg atcaccgaca cgcgcatggc gaccacgctg
     6961 tcggccggcg gcgacccggg cgcgcccaag gtcaagacca tcgagcacct gctgtcggcc
     7021 tgctcgggcc tgggcctgga caacctgatc gtcgacatca acggcgaaga ggtgccgatc
     7081 ctcgacggct cggccgcgag cttcgtgttc ctgctgcaaa gcgccggcat cgagctgcag
     7141 gacgcgccga agcgcttcgt gcgcatgctg agggccgtcg aggtgcgcga gggcgcgggg
     7201 ccggcgctga agtgggcgcg gctcgagccc ttcgagggct acaagctcac cttcgagatc
     7261 gacttcgacc acccggcggt cgaccagacc ggccagcgcg tcagcttcga catgggctcg
     7321 ggccgctaca agcaggagat cgcgcgcgcc cgcacgctcg gcttcacgaa ggacgtggac
     7381 atgatgcgcg cgcgcggcct ggcgctggga ggcagcatgg acaacgcgct cgtgatcgac
     7441 gactacaagg tgctcaacga cggcggcctg cgctacgacg acgagatcgt caagcacaag
     7501 atcctcgacg cgatcggcga catgctcgtg atcggccatc cgatgatcgg cgcctacagc
     7561 gcgttccgcg ccggccacga cctgaacaac aagctgctgc gcgcggtgct ggccgacccc
     7621 gccagcttcg atctcgtcag tttcgacgac gcgacgcgcg cgccggccgg cctggccgag
     7681 ctggcgccgg cctggtgagc gatgctgctg gttcgcgggc tcgtcgtgtt gctgctggtc
     7741 gccggtgcgg cgttcctggt cgcctacatg gtcaccgggc agaagcgtta tcaggccgcc
     7801 ggcgtgcgca tcgtcaagtg gacggtgatc gcggcgctcg gcttcttcgc cgtgctcatc
     7861 gtcgagcagc tggcgacgat gcgctgaggg gccgcgcatg acgatcttcc gctggatcat
     7921 cggcgtgctg gccgcgctgc ttgcgaccgg cgcgctgctc agcctcgcgc tgtttctcgc
     7981 gttcgacatc gaggtctggc tcaagcgcgc ccgcacgctg cgccacggcg tctacatggc
     8041 gctgctgctg tggttcaacg tcgagatctg gggccgggtg ctgggggcgc tcattcactg
     8101 gtgaaatgga gcccctcggc cgacgcgggc gtcggccgat cccccgaggg gatcgcgggc
     8161 cgccgaggtc cacgaggtgg gcctcttcgt ggcccatgga gcggcccggc gctcggcccg
     8221 ctcagattcc caggaaggcc atcacctcgg gcaggtgcgt gtcgaagtcg ctgagtgcgt
     8281 ggtcgccgcc ttcgagcagc ctgatcgcgg cgccgcggta gcgcgcgctc atctcgaccc
     8341 agctcagcag ctcgtcgccc tttgcgatga ccgcgaaata gcggtcgagc ctcgtcagcg
     8401 tcgccggtgc gatcgcgcgc agctcggcga tgaactcggc gcggaagaag aagcgctcgt
     8461 cgctgtgcca ggcggtggtc tccccgatgt aggtctgcag gtcgcgcgcc gggtcgatcg
     8521 ccgggttcag cagcaccgcg cggcagccca ggcgttcggc caccgcggtg gcgtagaagc
     8581 cgccgagcga gctgccgatc acgccgatgc ccgcggccgg ccagtcggcg acgccgtcca
     8641 tcacgagctg cagcgcgtcg cgcggcgagg ccggcagctg cgggcaccac cagtgcacgt
     8701 cgggccggtg ctgctgcagc cacgcggccg tcagccgcgc cttcgtcgat tgcggcgacg
     8761 agcggaagcc gtgcaggtaa agcagatgtg tgacggcgct cggcatcacc ctggctcagc
     8821 gcttggccag cgcgttcagc agcttcgcgt ggacgccgct gaaagccccg ttgctcatgc
     8881 acagcacgtg atcgcccggc cgcgccgcgg cgacgacgcg cttgacgagt tcatcgatgg
     8941 tgtcgcagac cactgcttgc gcgcccatcg gcgcgagcgc ctgggcggcg tcccagccca
     9001 gaccgccgct gtggcagaac gcgaggtcgg cctcttcgag cgaccacggc agctgggcct
     9061 tcatcgcccc gagcttcatc gtgttcgagc gcggctcgaa caccgcgagg atgcgctggc
     9121 gcggcgcgcc cggcgcggtc ttctcgtggt cgaccctgcg gcgcaggccg ttcacggtgg
     9181 tgcggatcgc ggtcgggtgg tgcgcgaagt cgtcgtagac cttgacgccg ccggcctcgc
     9241 cgcgcagctc gaggcggcgc cgcacgttct cgaagctcgc gagcgcgcgc ccggcgacgt
     9301 ccggcgcgac gccgacgtgc tcggccgcgg cgatcgcggc gagcgcattg agctggttgt
     9361 gctcgcccag cagcggccac tcgacgcgcg cgaccttcag gctgccgcgc agcacgtcga
     9421 aggcgtgcgg ctcgccgcgc gcgcgcagcg cgccgggctc ttccttgcgc gcgccgaagc
     9481 gcgtgacctc gctccagcag cccatcgcga gcacgcgctt gaggctgtcc tcgcgcgcgt
     9541 tcacgaccag ccgcccgtgc gccggcacgg tgcgcacgag gtgatggaac tggcgttcga
     9601 tcgcggcgag gtcgtcgaag atgtcggcgt ggtcgaactc gaggttgttc agcaccgcgg
     9661 tgcgcggccg gtagtgcacg aacttgctgc gcttgtcgaa gaacgcggtg tcgtactcgt
     9721 cggcctcgat gacgaacgcc gatcccggct cggcctggcc caggcgtgcc cctgggctcg
     9781 cagagcccag tcgtgcggaa acgccgaagt tcagcggcac gccgccgacc aggaagcccg
     9841 gcgccaggcc ggcctgttcg aggatccagg ccagcatcga cgtggtcgtc gtcttgccgt
     9901 gggtgccggc caccgcgagc acgtggcgac cttgcagcac gtggtcggcg agccactgcg
     9961 ggccgctggt gtacggggcg ccggcgtcga ggatcgcctc catcagcggg ttgccgcgca
    10021 cgatcacgtt gccgatcacg aacaggtcgg gccggaaggc gagctgctcg gcgccgtagc
    10081 cttcgacgag ctcgatgccg agcgcgcgca gctggtcgct catcggcggg tagacgttcg
    10141 tgtcgcagcc ggtgacggtg tggccgcctt cgcgcgcaag cgctgccagc ccacccatga
    10201 aggtgccgca gatgccgagg atgtggatgt gcatggaagg agttccggaa gatgagtcgc
    10261 gccgccgtgc cgctgcgtca cgccgcgccg acgcggccga agccgaacca ttctgcgccg
    10321 tgtgcgccga ccgcttcgag cgagggcaat accgccaagc aacgggcttg ccaggccggc
    10381 tcgggcgcca gcagatccgg caccgcgacg accgcgaggc cggcctcgag cgccgcctgc
    10441 acgccgtgcc cggagtcctc gaacgcgagc gcggtcgccg ccgcggcgcc gaggcgggcc
    10501 actgccagcg cgtacagatc gggcgcgggt ttgccgcagc gaacctcgtc gccgccgcag
    10561 accgccgcga agaagtcgag cagcccggcc ttgcccaggc ggcgctgcac ttcgccgcgg
    10621 tgcgtcgatg acgcgaccgc gcatggcacg cgcgccgcgt gcagcctgcg cagcagcgcg
    10681 agcgcgccgg gcttcgtgtc gaagggacga tcgccgaagc gctcgttcag ctgggattcg
    10741 accaggtcgc cggcggcggc cagccgcgcc gcatcgccgc cgaacagtcg cagcaggcgc
    10801 gcgccgctgt cgacgtggtt caggccgatc agcgtcaggt agtcggcgtc gcccagcggc
    10861 acgccgagcg tcgcggccgc gc
//
LOCUS       DATMJB010000061         4480 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_464257, whole genome shotgun sequence.
ACCESSION   DATMJB010000061 DATMJB010000000
VERSION     DATMJB010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464257"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VNU48_01460"
     CDS             complement(<1..965)
                     /locus_tag="VNU48_01460"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWI09976.1"
                     /translation="MTTPDIFIIAAARTAIGTYGGALKDVPLTQLATTVVREAIVRSG
                     VTAADIGHVVMGNVIPTEPRDAYLARVAAIEAGIPQETPAFNVNRLCGSALQAIISAA
                     QTLMLGDAEIAIGGGAESMSRSPYLMPAARWGARMGDVQTLDYMLGILQDPFHKFHMG
                     ITAENVAAREGITREMQDSLALESQKRAARAIVEGRFTSQIAAVEIATRKGMVVFDTD
                     EHVRGDVTAEKLAKMKPAFKGDGSVTAGNASGVNDGAAALVLATGEAVRSRGLKPMAR
                     LVSYAHVGVDPKLMGLGPIPASRLALQRAGLTVADLDVIESNEAFA"
     gene            complement(962..1312)
                     /locus_tag="VNU48_01465"
     CDS             complement(962..1312)
                     /locus_tag="VNU48_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09977.1"
                     /translation="MGSIGAACRPAAMSRRIIRDWKRSISIGLQPQAEPPAALLAKES
                     ALSLLQRSVSFGHGRLAILRLAIAVQSGADVPAAHWSYCRNAASASGDQTLQTLLERA
                     ESAAQTLVTSQPTP"
     gene            complement(1287..>4480)
                     /gene="icmF"
                     /locus_tag="VNU48_01470"
     CDS             complement(1287..>4480)
                     /gene="icmF"
                     /locus_tag="VNU48_01470"
                     /EC_number="3.6.5.-"
                     /EC_number="5.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0047727 - isobutyryl-CoA mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fused isobutyryl-CoA mutase/GTPase IcmF"
                     /protein_id="HWI09978.1"
                     /translation="INIMRRILQGMGCEVVHLGHDRSVDEVVSAALQEDAHGVAVSSY
                     QGGHVEYFKYMVDLLKSRGGEHIQVFGGGGGVIVGSEIAELQGYGVTRIYSPEDGQRM
                     GLAGMIGEMVMRCDVELSGHAPKAIAAIQGQTEAHWRALAQLITALENGQASDAIKTA
                     LHAEAQKARTPVLGITGTGGAGKSSLTDELIRRLRLDQNDALRIAVISIDPSRRKSGG
                     ALLGDRIRMNAIQPWSQGQRVFMRSLATRDFGSEISQALPDVLAAVRASAGFDLIIVE
                     TSGIGQGDAAIVPLVDVPMYVMTPEFGAASQLEKIDMLDFAEFVAINKFDRKGAADAL
                     RDVAKQVQRNQEAWKTPPDEMPVFGTMASRFNDDGVTALYQALLPRLAQLGMKVGPGA
                     LPIAATRHSTHQVPIVPGARVRYLADIADTVRGYKKHAHAQAALARAIQQLRATAAML
                     HTDKPERHRAVEAVDELARTREAGFDPRAAKLLAMWPQMQRAYAGDEYVVKIRDKELR
                     TALTYTSLSGTKVRKVALPTFEDHGEILKWLMLDNVPGSFPYTAGTFAFKREGEDPTR
                     MFAGEGDAFRTNTRFKLLSSGMAAKRLSTAFDSVTLYGNDPAVRPDIYGKVGNSGVSI
                     ATLDDMKVLYSGFDLTNPATSVSMTINGPAPTILAMFMNTAIDQNLDKFRADNGREPT
                     ADEAQKIVAWVKENVRGTVQADILKEDQGQNTCIFSTEFSLKVMGDIAEYFVHHKVRN
                     FYSVSISGYHIAEAGANPISQLAFTLSNGFTFVEAYLARGMHIDDFAPNLSFFFSNGM
                     DPEYTVLGRVARRIWAVAMRDRYGANERSQKLKYHVQTSGRSLHAQEIQFNDIRTTLQ
                     ALIAIYDNCNSLHTNAFDEAITTPTEDSVRRAMAIQLIINREWGLAKNENPNQGAFII
                     DELTELVEEMVLQEFEKIAERGGVLGAMETGYQRGKIQEESMHYEMLKHTGEYPIVGV
                     NTFRNPHGDPVADTLELARSTDEEKQSQLKRLAEFQQRHAAESPAMLRRLQQAVIENR
                     NVFEVLMEAVRCCSLGQITNALFEVGGQYRRSM"
BASE COUNT          621 a         1517 c         1505 g          837 t
ORIGIN      
        1 gcgaaggctt cgttggattc gatcacgtcg aggtcggcga ccgtcaggcc tgcgcgctgc
       61 agcgcgagcc gcgatgccgg gatcgggccc aggcccatca gcttcggatc gacgccgacg
      121 tgagcgtagc tgaccaggcg cgccatcggc ttcagcccac gggaccggac cgcctcgccc
      181 gtggcgagca ccagcgcagc tgcgccatcg ttgacgcccg atgcgttgcc ggccgtcacc
      241 gagccatcgc ccttgaaggc gggcttcatc ttcgccagtt tctcggccgt gacgtcgccg
      301 cgcacgtgct catcggtgtc gaacaccacc atgcccttgc gcgtcgcgat ctcgaccgcc
      361 gcgatctgcg atgtgaagcg cccctccacg atggcgcgcg cggcgcgctt ctggctttcg
      421 agagcgaggc tgtcctgcat ctcgcgggtg atgccttcgc gtgccgcgac gttctctgcg
      481 gtgatgccca tgtggaactt gtggaacggg tcttgcaaaa taccgagcat gtagtccagc
      541 gtctgcacgt cgcccatgcg cgcgccccaa cgagcggccg gcatgaggta ggggctgcgg
      601 ctcatcgact ctgcaccacc gccgatggcg atttctgcgt cgccgagcat cagcgtctgt
      661 gcggccgaga tgatggcttg gagtgccgaa ccgcacaggc ggttgacgtt gaaggccggc
      721 gtctcctgcg ggatgccggc ttcgatcgcg gcaacgcggg cgaggtaggc gtcacggggt
      781 tccgtgggga tcacatttcc catcacgacg tggccgatgt cggccgcggt cacgccgctg
      841 cggacgatcg cttcacgcac gacggtggtc gcgagctgcg tcagtggcac gtccttgagt
      901 gcaccaccgt aggtgccgat cgcggtgcga gccgcggcga tgatgaagat gtcgggtgtg
      961 gtcatggtgt gggctgagaa gtgacaaggg tctgtgccgc ggactctgcg cgttccagca
     1021 gggtctgcaa ggtctggtcg ccggacgccg aggcggcatt gcgacaatag ctccagtgcg
     1081 ccgccggcac gtcggcgcca ctctgcaccg cgatcgccag gcgcaggatg gcaaggcgcc
     1141 cgtggccgaa gctgacgctg cgctgaagca gcgagagcgc cgattctttg gccagcagtg
     1201 cggccggcgg ctcggcctgc ggctgcaggc cgatggagat ggaacgcttc cagtctcgaa
     1261 tgatccgtcg tgacatggct gccggcctac atgctgcgcc gatactgccc accgacctcg
     1321 aacagcgcat tcgtgatctg ccccagcgag cagcaacgca ccgcctccat cagcacctcg
     1381 aacacgttgc ggttctcgat caccgcctgc tgcagccgcc gcagcatcgc gggcgactcg
     1441 gcggcgtggc gctgctggaa ctccgccagt cgcttcaact gactctgctt ctcttcgtcg
     1501 gtcgagcgcg ccagctccag cgtgtcggcg accgggtcgc cgtgcgggtt gcggaaggtg
     1561 ttcacgccca cgatcggata ctcaccggtg tgcttgagca tctcgtagtg catgctctct
     1621 tcctggatct tgccgcgctg gtagcccgtc tccatcgcgc cgagcacgcc gccgcgctcg
     1681 gcgatcttct cgaactcctg cagcaccatc tcctccacga gttcggtcag ctcgtcgatg
     1741 atgaacgcac cctggttcgg gttctcgttc ttcgccagac cccactcgcg gttgatgatc
     1801 agctggatcg ccatcgcgcg gcgcacgctg tcttcggtcg gcgtcgtgat cgcctcgtcg
     1861 aacgcgttgg tgtgcagcga gttgcagttg tcgtagatcg cgatcagcgc ctgcagcgtg
     1921 gtgcggatgt cgttgaactg gatctcctgc gcgtgcaggc tgcgccccga ggtctgcacg
     1981 tggtacttca gcttctggct gcgctcgttc gcgccgtagc gatcccgcat cgccaccgcc
     2041 cagatgcgcc gcgcgacccg cccgagcacc gtgtactccg ggtccatgcc gttgctgaag
     2101 aagaagctca ggttcggcgc gaagtcgtcg atgtgcatgc cgcgcgcgag atacgcctcg
     2161 acgaaggtga agccgttgct cagcgtgaac gccagctggc tgatcgggtt cgcgccggcc
     2221 tcggcgatgt gatagccgct gatgctcacc gagtagaagt tgcgcacctt gtggtgcacg
     2281 aagtactcgg cgatgtcgcc catcaccttc aggctgaact cggtgctgaa gatgcaggtg
     2341 ttctggccct ggtcttcctt caggatgtcg gcctgcaccg tgccgcgcac gttctccttg
     2401 acccaggcga cgatcttctg cgcttcgtcg gcggtgggct cgcggccgtt gtcggcgcgg
     2461 aacttgtcca ggttctgatc gatcgcggtg ttcatgaaca tcgccaggat cgtcggcgcc
     2521 gggccgttga tcgtcatgct cacgctggtg gccgggttcg tcaggtcgaa gcccgaatac
     2581 agcaccttca tgtcgtcgag cgtggcgatg ctcacgcccg agttgccgac cttgccgtag
     2641 atgtcgggcc gcaccgccgg gtcgttgccg tacagcgtga ccgagtcgaa cgcggtgctc
     2701 aggcgcttcg ccgccatccc cgagctcaag agcttgaagc gcgtgttggt gcggaaggcg
     2761 tcgccctcgc cggcgaacat gcgcgtcggg tcttcgccct cgcgcttgaa cgcgaaggtg
     2821 ccggcggtgt acgggaagct gcccggcacg ttgtccagca tcagccactt caggatctcg
     2881 ccgtggtctt caaaggtcgg cagcgcgacc ttgcggacct tcgttcccga cagcgaggtg
     2941 taggtcagcg cggtgcgcag ctccttgtcg cggatcttca ccacatactc gtcgccggcg
     3001 taggcgcgct gcatctgcgg ccacatcgcc agcagcttgg cggcacgcgg gtcgaacccg
     3061 gcctcgcgtg tgcgggccag ctcgtcgacc gcctcgaccg cgcggtgacg ctccggcttg
     3121 tcggtgtgca gcatggcggc ggtcgcgcgc agctgctgga tcgcgcgcgc cagcgcggcc
     3181 tgcgcgtgcg catgtttctt gtagccgcgc accgtgtcgg cgatgtcggc gaggtagcgc
     3241 acgcgcgcgc ccggcacgat cggcacctgg tgcgtgctgt ggcgtgtcgc ggcgatcggc
     3301 agcgcgccgg ggccgacctt catgccgagc tgcgccagcc gcggcagcag cgcctggtac
     3361 agcgcggtca cgccgtcgtc gttgaagcgg ctggccatcg tgccgaacac cggcatctcg
     3421 tcgggcggcg tcttccaggc ctcctggttg cgctgcacct gcttggccac atcgcgcagc
     3481 gcatcggccg cgcccttgcg gtcgaacttg ttgatcgcga cgaactcggc gaagtccagc
     3541 atgtcgatct tctcgagctg gctggcggcg ccgaactcgg gcgtcatcac gtacatcggc
     3601 acgtccacca gcggcacgat cgccgcgtcg ccctggccga tgcccgaggt ctcgacgatg
     3661 atcaggtcga agcccgcgct ggcgcgcacc gcggccagca cgtcgggcag cgcctggctg
     3721 atctcgctgc cgaagtcgcg cgtggccaac gagcgcatga agactctttg accctggctc
     3781 cagggctgga tcgcgttcat gcggatgcgg tcgccgagca gcgcgccgcc gctcttgcgc
     3841 cggctcgggt cgatgctgat gaccgcgatg cgcagcgcgt cgttctggtc cagccgcagg
     3901 cgccggatca gctcgtcggt caggctcgac ttgccggcgc cgccggtgcc ggtgatgccc
     3961 agcaccgggg tcctggcctt ctgcgcttcg gcatgcagcg cggtcttgat cgcgtcgctg
     4021 gcctggccgt tctcgagcgc ggtgatcagc tgcgcgagtg cgcgccagtg cgcttcggtc
     4081 tggccctgga tcgccgcgat cgccttgggc gcatggcccg acagctccac atcgcaccgc
     4141 atcaccatct cgccgatcat gccggccagg cccatgcgct ggccgtcctc ggggctgtag
     4201 atgcgcgtca cgccgtagcc ctgcagctcg gcgatctcgc tgcccacgat cacgccgccg
     4261 ccgccgccga agacctggat gtgttcgccg ccgcggctct tcagcaggtc gaccatgtac
     4321 ttgaagtact cgacgtggcc gccctggtag ctgctgacgg cgacgccgtg cgcatcctcc
     4381 tgcagcgcgg cgctgacgac ttcgtcgacc gagcggtcgt ggccgaggtg gacgacctcg
     4441 cagcccatgc cctgcaggat gcgccgcatg atgttgattg
//
LOCUS       DATMJB010000062         7833 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_168326, whole genome shotgun sequence.
ACCESSION   DATMJB010000062 DATMJB010000000
VERSION     DATMJB010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168326"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..1206
                     /gene="dnaJ"
                     /locus_tag="VNU48_01475"
     CDS             61..1206
                     /gene="dnaJ"
                     /locus_tag="VNU48_01475"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWI09979.1"
                     /translation="MAKRDYYETLGVPKNASEDDIKKAYRKLAMKHHPDRNQGDDAKK
                     SEEKFKEAKEAYEMLTDAQKRAAYDQYGHAGVDPNMGRGGGPGAEGFGGFAEAFGDIF
                     GDIFGGAAAGGQRRGGQQVFRGSDLSYAMEITLEEAAHGKETQIRIPSYETCETCKGS
                     GAKPGTSPKVCSTCNGQGTVHMRQGFFSIQQTCPHCHGTGKIIPEPCTTCSGQGRVKK
                     NKTLEVKIPAGINEGMRIRSSGNGEPGTNGGPAGDLYIEIRIKQHEIFERDGDDLHCT
                     IPVGLTTAALGGGIEVPTLGGKAEIELPEGTQHGKTFRLRGKGIKGVRSSYPGDLYCH
                     VSVETPIKITEHQRKLLKELDESFRSSGDRHSPNAKTWTDRVKDLFK"
     gene            1481..2320
                     /locus_tag="VNU48_01480"
                     /pseudo
     CDS             1481..2320
                     /locus_tag="VNU48_01480"
                     /inference="COORDINATES: protein motif:HMM:NF019631.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase
                     domain-containing protein"
     gene            2424..2936
                     /locus_tag="VNU48_01485"
     CDS             2424..2936
                     /locus_tag="VNU48_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWI09980.1"
                     /translation="MLLVLHQMGSHGPAYYKRSPPQRKPFMPECTTNVLQQCEHQALV
                     NAYDNSIAYTDHVLAQTIAWLQQQQPQFDTALLYLSDHGESLGENNLYLHGLPYALAP
                     VEQKHVPMLLWLGPQASADGRLPPACLNAQRDAALSHDNLFHTTLGLLGIRAAEYRQN
                     LDAFAACRAP"
     gene            2977..3885
                     /locus_tag="VNU48_01490"
     CDS             2977..3885
                     /locus_tag="VNU48_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWI09981.1"
                     /translation="MAAQADSLKSIFFALGANFAIALAKSAGAVFTGSSSMLAEAIHS
                     FADCANQALLIWGLKEAKREASAEHPLGHGRAIYFWSFIVALMLFSMGGLFSIYEGVH
                     KLHDSEPVRYAWVAVGILAFGVAAESVSLWGALREINKERGAASLWQWFRQSRQSELL
                     VIFGEDLAALGGLALALGFILLSIVTGDPVWDAVGSICIGVLLVLVAILVGIEVKALL
                     VGQSAEPQVLEALRAHLVAQPQVAQLYSLITQQLGSEIMLAVKARMQPQASAVALIEA
                     INAVERGLRAAFPQLRWVFFEPDLRD"
     gene            complement(3895..5034)
                     /locus_tag="VNU48_01495"
     CDS             complement(3895..5034)
                     /locus_tag="VNU48_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HWI09982.1"
                     /translation="MQRPRRIEATERDDPVANARAPLKGRGTVWSIQHRYTQQASEAY
                     DDGWGTLDQQASEEMLPPSTTIIEERVKSILSSNDSPDISFDLSINPYRGCEHGCIYC
                     FARPTHSYLNLSPGLDFETRIIAKVNAAERLREAFASRGYKPLLLNLGSATDAYQPAE
                     RRLRITRSVIEVLAECAHPFSLVSKSCGVERDLDLIAPMAQRGLAAVYMSVTSLDPAL
                     ARILEPRAASPHRRLQTIETLAKAGVPVGVSVSPIIPFINEPELERILEAAAAAGASS
                     AFSIVLRLPWEVNPLFQQWLERHFPQRAARVMARVREMRGGRDYDARFGARMTGEGVW
                     AQLLRQRFEKTAARLGLNRERVELDLTQFRRPLEPRADGQQDLFA"
     gene            5140..5601
                     /locus_tag="VNU48_01500"
     CDS             5140..5601
                     /locus_tag="VNU48_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI09983.1"
                     /translation="MPIQHINHGSTHGYQVRVGPRHAALTRFFAVRKYGGARKALAQA
                     RAAESELQKSAPSVSPREGPRVVAQANNTSGLVGIRPRYVQFSEHPYLYFVASWSLNG
                     KPRSTSFSAERHGMLVALQMAMERREKATGLKYEMTARQALNRMKHLLSAR"
     gene            5802..6311
                     /locus_tag="VNU48_01505"
     CDS             5802..6311
                     /locus_tag="VNU48_01505"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HWI09984.1"
                     /translation="MLIGLRESAPTLLLVLGCSAAAALWLWRHSRAPGLVNDARVAPV
                     RRADREAWREFETLVAEAFRRQGYQVLDSTRGTAESGGELTLRRERETFLVQCKHWKE
                     AKVGVDAVHALQRAMTARGASGGFMLTDGRFAREAIAFANGCNVRLIDGAALQGLLDQ
                     AKGSARGAR"
     gene            complement(6511..>7833)
                     /locus_tag="VNU48_01510"
     CDS             complement(6511..>7833)
                     /locus_tag="VNU48_01510"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWI09985.1"
                     /translation="PFELESAHQGEEALDKVEDSLAAERPYAMAFVDMRMPPGWNGVE
                     TVERLWQADPRLQIVLCTAHSDLPWDEVLARLAVRDRLLILKKPFDPIEVYQLANALC
                     TRWDLSRLEDAVAQRAGELNATNAALRRDIIERKRAEAELRLAASAFHDTMDGVMIVD
                     PAGVVLSVNPAFAAMTGYPVEQMPGRPAHLLRCGRQSARFYRDLWTTLARVGRWQGEL
                     WTRRHDGEVFLAWLNIVTVTGSDGRAERHVCVFHDITTLRRNEDRIRHLAFHDPLTGL
                     PNRALLQDRLDQAIASTRRDRESLGLLFIDLDRFKVVNDSFGHDAGNALLKEVAGRLT
                     GCLRRSDTVARIGGDEFVLLLRGLGMPQSYALVAQAAIDALSVPMVLDGHAMQVGASI
                     GIACFPDDGLDAATLMTHADMAMYAAKAAGRGTYRFFQPSLADTARPL"
BASE COUNT         1244 a         2715 c         2713 g         1161 t
ORIGIN      
        1 gtgacaggct ttgcctgcac gtcgacgcgg cggttccgtt tcgatgcgct gagaaatttc
       61 atggccaaac gcgactacta cgaaacgctc ggcgtcccga agaacgccag cgaagacgac
      121 atcaagaagg cctatcgcaa gctcgcgatg aagcaccacc cggaccgcaa ccagggtgac
      181 gatgcgaaga agtcggaaga gaagttcaag gaggccaagg aggcctacga gatgctcacc
      241 gacgcgcaga agcgcgccgc ctacgatcag tacggccatg cgggcgtcga ccccaacatg
      301 ggccgcggcg gcggccccgg cgccgaaggc ttcggcggct tcgccgaggc cttcggcgac
      361 atcttcggcg acatcttcgg cggcgcggcc gccggcggcc agcgccgcgg cggccagcag
      421 gtgttccgcg gcagcgacct gagctacgcg atggagatca cgctcgaaga agcagcgcac
      481 ggcaaggaga cgcagatccg catcccgtcg tacgagacct gcgagacctg caagggcagc
      541 ggcgccaagc ccggcaccag cccgaaggtc tgcagcacct gcaacggcca gggcacggtg
      601 cacatgcgcc agggcttctt cagcatccag cagacctgcc cgcactgcca cggcacgggc
      661 aagatcatcc ccgagccctg caccacctgc agcggccagg gccgggtcaa gaagaacaag
      721 acgctggagg tgaagatccc cgccggcatc aacgagggca tgcgcatccg ctccagcggc
      781 aacggcgaac ccggcacgaa cggcgggccg gccggcgacc tctacatcga gatccgcatc
      841 aagcagcacg agatcttcga gcgcgacggc gacgacctgc attgcacgat ccccgtcggg
      901 ctcacgaccg ccgcgctggg cggcggcatc gaggtgccca cgctcggcgg caaggccgag
      961 atcgagttgc ccgagggcac gcagcacggc aagaccttcc gcctgcgcgg caagggcatc
     1021 aaaggcgtgc gctcgagcta cccgggcgac ctttactgcc acgtgagcgt cgagaccccg
     1081 atcaagatca ccgagcacca gcgcaagctg ctgaaggagc tggacgagtc gttccgcagc
     1141 tcgggcgacc gccattcgcc gaacgcgaag acctggaccg atcgggtcaa agacctgttc
     1201 aagtgaccgc cgcgcagccg ccctgcccgg caatgcacgg acaatggcgg ccatgacgaa
     1261 gcgcaggacg ttcgccgccg ggctcggcgg cgccgcatcg gccggctgga acccgctgac
     1321 gctggccgtg ctggccggca tatggatcgc ggccttgccg aactggccgc tgtggcgtgc
     1381 gctgctggcg ctgcccgaga ccgcctcggc gcgcggcgcg atgttcgtcg ccggcttcgg
     1441 cctgatggtg gcggcgctga ccgccctgct gctcgcgccg atggcttggc gcgtgacgat
     1501 caagccggcg atcgcggtgc tggtgctggc cgcggctttc ggcgcgcact tcatggggac
     1561 ctacggcgtc gtcatcgaca cgacgatgat gaccaacgtc gtccagaccg atccgcgcga
     1621 ggtgcgcgac ctgctgagcc tgcgcctgct gggcagcgtg ctgctgctcg ccggcgtgcc
     1681 gctgctgctg ctgtggcgcg cgccggtgcg cggcatcggg gcgtggcgcc aggccgggcg
     1741 caatgcgctg ggcatcgtcg cgctcgcgct gctgctcgcg gcactgctgt tcgcgctgtt
     1801 cgccgacctg tcgacgacga tgcgcaacca ccgctcgatg cgctatctga tcaacccggt
     1861 gaattcgctg tacgcgctgg tcgacctcgc ggcgcagtcg caggcccggc ccagcggccc
     1921 gccgctggcg atcggcgccg acgcgcgcat cctgccgcgg ccggcgggcg cgaagccgcc
     1981 gctgctggtg ctcgcgatcg gcgagaccgc gcgcgccgac cacttcgcgc tgaacggcta
     2041 tgcgcggccg acgaacccgg agctggccgc gctgaacgtg atcagctttc gccacgtgat
     2101 gtcctgcggc accaacaccg cggcctccct gccctgcatg ttctcggcgc tgggcaagag
     2161 cgcgttcgag tcgcgcgggc gcgagcacga gaacctgctc gacctggtgc agcgcgccgg
     2221 catggcggtg ctgtggatcg acaaccaggc cggctgcaag ggcctgtgcg agcgcgtgcc
     2281 gcatgccttc gcacgcgagc cggtggccgg tgtgccggcg ctgccggcag cgctgtgcga
     2341 cggcgacgaa tgcctggacg acgcgctgct gcacggcatg gccgcgcgcc tggccgcgct
     2401 gcccgccgcc gcgcgcgcgc ggcgtgctgc tggtgctgca ccagatgggc agccacggcc
     2461 cggcctacta caagcgcagc ccgccgcagc gcaagccgtt catgcccgaa tgcacgacca
     2521 acgtgctgca gcagtgcgag catcaggcgc tcgtcaacgc ctacgacaac tcgatcgcct
     2581 acaccgacca cgtgctggcg cagacgatcg cctggctgca gcaacagcag ccgcagttcg
     2641 acaccgcgct gctgtacctg tcggaccacg gcgagtcgct gggcgagaac aacctgtacc
     2701 tgcacggcct gccgtacgcg ctcgcgccgg tcgagcagaa gcacgtgccg atgctgctct
     2761 ggctgggccc gcaggcatcg gccgacgggc gactgccacc cgcgtgcctg aacgcacagc
     2821 gcgacgcggc gctgagccac gacaacctgt tccacaccac gctcgggctg ctgggcatcc
     2881 gcgccgccga gtaccggcag aatctggacg cgttcgccgc ctgccgcgcg ccctgatctc
     2941 gcgcgccgcc gaacaccaga cctttcaagg agaaccatgg ccgcccaagc cgactcgctc
     3001 aagtcgatct tcttcgcgct cggcgccaac ttcgcgatcg cgctggcgaa gagcgccggc
     3061 gcggtcttca ccggctcgtc gtcgatgctg gccgaggcca tccattcgtt cgccgattgc
     3121 gccaaccagg cattgctgat ctggggcctg aaggaggcca agcgcgaggc gtccgccgag
     3181 cacccgctcg gtcacggtcg cgcgatctac ttctggagct tcatcgtcgc gctgatgctg
     3241 ttcagcatgg gcgggctgtt ctctatctac gaaggtgtgc acaagctgca cgacagcgaa
     3301 ccggtccggt acgcctgggt ggcggtgggc atcctggcgt tcggcgtcgc ggccgagtcg
     3361 gtgtcgttgt ggggcgcgct gcgcgagatc aacaaggagc gcggcgcggc gagcctgtgg
     3421 cagtggttcc gccagtcgcg ccagagcgag ctgctggtga tcttcggcga agacctcgcc
     3481 gcgctcggcg gcctggcgct ggcgctcggt ttcatcctgc tgtcgatcgt caccggcgac
     3541 cccgtgtggg acgccgtcgg ctcgatctgc atcggcgtgc tgctggtgct ggtggcgatc
     3601 ctggtcggca tcgaggtcaa ggcactgttg gtcggccaaa gcgccgaacc gcaggtgctc
     3661 gaggcgctgc gcgcgcacct cgtcgcgcag ccgcaggtcg cgcagctcta cagcctgatc
     3721 acgcagcagc tcggcagcga gatcatgctg gccgtcaagg cacgcatgca gccgcaggcg
     3781 tcggccgtgg cgctgatcga ggcgatcaac gcggtcgagc gcgggctgcg tgcggccttt
     3841 ccgcagctgc gctgggtctt cttcgagccc gacctgcgcg actgagcgcg cgcgtcaggc
     3901 gaacaggtcc tgctggccgt cggcgcgcgg ctcgagcggc cggcggaact gcgtcaggtc
     3961 gagctcgacg cgctcgcggt tcaggccgag gcgcgccgct gtcttctcga aacgctggcg
     4021 cagcagttgc gcccagacgc cctcgcccgt catgcgcgca ccgaagcgcg cgtcgtagtc
     4081 gcggccgccg cgcatctcgc gcacacgcgc catcacgcgc gcggcgcgct gcgggaagtg
     4141 tcgctccagc cattgctgga acaacgggtt cacctcccag ggcaggcgca gcacgatgct
     4201 gaaggccgag ctcgcgccgg cggccgccgc agcttcgagg atgcgctcga gctcgggctc
     4261 gttgatgaaa gggatgattg gcgagacgct cacgcccacc ggcacgcccg ccttggccag
     4321 cgtctctatc gtttgcaggc ggcgatgcgg cgaggccgcg cgcggctcga ggatgcgcgc
     4381 cagcgcgggg tcgagcgaag tcaccgacat gtacaccgcg gcgaggccgc gctgcgccat
     4441 cggcgcgatc aggtcgaggt cgcgctcgac gccgcaggac ttgctcacca gcgagaacgg
     4501 gtgcgcgcat tcggccagca cctcgatcac cgagcgcgtg atgcgcagcc tgcgctcggc
     4561 cggctggtag gcgtcggtgg ccgagcccag gttcagcagc aggggtttgt agccgcggct
     4621 ggcgaacgcc tcgcgcaggc gctcggcggc gttgaccttc gcgatgatgc gggtctcgaa
     4681 gtccagcccc ggcgagaggt tcaggtagct gtgcgtgggc cgcgcgaaac agtagatgca
     4741 gccatgctcg cagccgcggt acgggttgat cgacaggtcg aacgagatgt cgggcgagtc
     4801 gttgctcgac aggatggact tgacgcgttc ctcgatgatc gtggtcgacg gcggcagcat
     4861 ctcctcggaa gcctgctggt cgagcgtgcc ccagccgtcg tcataggcct cgctggcctg
     4921 ctgcgtgtag cggtgctgaa tcgaccacac cgtgccgcgg cccttgagcg gcgcgcgtgc
     4981 attcgcgacg gggtcgtcac gctctgtcgc ctcgattcga cgcggtctct gcatactgtg
     5041 aatatataca gtttcatcta atataccaag gccggcgggt cggcatcatc aatcctgagg
     5101 atgacattgc ccttatcctt cgttccagaa ggagtcctca tgccaataca acacatcaac
     5161 catggctcga cccatggcta ccaagttcgc gttgggccgc ggcacgcggc gttgacccga
     5221 ttctttgccg tgcgcaagta cggcggcgcc cgcaaggcgc tggcgcaggc gcgcgctgcc
     5281 gagagcgaac tgcaaaagtc ggcgccgtcg gtctcgccgc gggaggggcc gcgcgtggtc
     5341 gcgcaggcca acaacaccag cgggctggtc ggcatccggc cgcgctatgt gcagttctcg
     5401 gagcacccgt acctgtactt cgtcgcatcc tggagcctca acggcaaacc ccgctcgacg
     5461 agtttctcgg ccgagcggca cggcatgctg gtcgcgctgc agatggcgat ggagcggcgc
     5521 gaaaaggcca ccggactgaa gtacgagatg accgcgcgcc aggcactgaa ccggatgaaa
     5581 cacctgctca gcgcgcgctg agacgggccg gccgggccgg cgctggagcc cctcgcggcc
     5641 gcgcatcgcg tgacgcactg cgcgcacgcg ctgcgcaccg cagcgtcgcg aaacgctaga
     5701 gtgatcggca acgcattgca gttcgccgac accccgatgg ccccaccttt ccagcacacc
     5761 gccaacggtt cgaccgacct cattcgccac gggctcgcgc gatgctgatc ggcctgcgcg
     5821 agtccgcccc gaccctgctg ctggtgctcg gctgcagcgc tgccgccgcg ctctggctct
     5881 ggcgccattc gcgtgcaccc ggtctggtca acgacgcgcg cgtggcgccg gtgcgccgtg
     5941 ccgatcgcga agcctggcgc gaattcgaga cgctggtcgc cgaggccttt cgccggcagg
     6001 gctaccaggt tctcgacagc acgcgcggca ctgccgagag cggcggcgaa ctgaccctgc
     6061 ggcgcgagcg cgagaccttt ctcgttcagt gcaaacattg gaaggaagcc aaggtcggcg
     6121 tagacgcggt gcacgcgctg caacgcgcga tgacggcgcg cggcgcgagc ggtggcttca
     6181 tgctcaccga cggtcgtttc gcgcgcgagg cgatcgcgtt tgcgaacggc tgcaatgtgc
     6241 gcctgatcga tggcgccgcg ctgcagggcc tgctggatca ggccaagggc agcgcgcgcg
     6301 gcgcgcggta accgcaggca actgcgcccg gcgttctcga caggcgcagc acccgatcgc
     6361 ccacccggat tgatggcgcc tcgcggcgcc cgccaactaa cgcggagctc acgctccccc
     6421 aggggccgtg ggctctccat ctccgcatgg agaaccacaa tggcaaggaa agccacggag
     6481 gcgcgagtac ggagagttct gcatctcgtg ctagaggggc cgcgccgtgt ccgccaagct
     6541 tggctggaaa aagcggtagg taccgcggcc ggcggccttg gcggcgtaca tcgccatgtc
     6601 ggcatgcgtc atcagcgtgg cggcgtcgag cccgtcatcc ggaaagcagg caatgccgat
     6661 cgaggcgccg acctgcatcg catggccgtc gagcaccatc ggtaccgaca gcgcatcgat
     6721 cgcggcctgc gccaccagcg cgtagctctg cggcatcccg aggccgcgca gcagcagcac
     6781 gaactcgtca ccaccgatgc gcgcgacggt gtcggagcgg cgcaggcagc cggtcagccg
     6841 cccggccact tccttcagca gcgcattgcc ggcgtcgtgg ccgaacgagt cgttgacgac
     6901 cttgaagcgg tcgaggtcga tgaacaggag gccgagcgat tcgcggtcgc gccgcgtcga
     6961 ggcgatcgcc tggtccagcc gatcctgcag cagcgcgcgg ttcggcaggc cggtcagcgg
     7021 atcgtggaag gcgagatggc ggatgcggtc ctcgttgcgc cgcaaggtgg tgatgtcgtg
     7081 gaatacgcag acgtggcgtt cggcacggcc gtcgctgccg gtgaccgtca cgatgttcag
     7141 ccaggcgagg aagacctcgc cgtcgtggcg ccgggtccac agctcgccct gccatcggcc
     7201 cacgcgggcc agcgtggtcc acagatcgcg atagaagcgc gcgctctgcc gcccgcagcg
     7261 caacaggtgg gccggccggc ccggcatctg ctcgacgggg tagccggtca tcgcggcgaa
     7321 ggccggattg accgacagga cgacgcccgc cgggtcgacg atcatcacgc cgtccatcgt
     7381 gtcgtggaac gcgctggcgg cgagccggag ctctgcctcg gcgcgcttgc gttcgatgat
     7441 gtcccgccgc agcgcggcgt tggtcgcgtt caactcgccc gcgcgctgcg ccaccgcatc
     7501 ttcgagccgc gacaggtccc accgggtgca tagggcattg gcgagctggt agacctcgat
     7561 cgggtcgaag ggcttcttca ggatcagcag ccggtcgcgc acggccagcc gcgccagcac
     7621 ctcgtcccac gggagatccg aatgggctgt gcagagcacg atctgcagcc gcgggtcggc
     7681 ctgccacagg cgctcgaccg tctccacgcc gttccagccg ggcggcatgc gcatgtcgac
     7741 gaaggccatc gcgtacggcc gctcggcggc cagcgagtcc tccaccttgt ccagcgcttc
     7801 ctcgccctga tgggccgact cgagttcgaa ggg
//
LOCUS       DATMJB010000063         3214 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_345873, whole genome shotgun sequence.
ACCESSION   DATMJB010000063 DATMJB010000000
VERSION     DATMJB010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345873"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1799
                     /locus_tag="VNU48_01515"
     CDS             42..1799
                     /locus_tag="VNU48_01515"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWI09986.1"
                     /translation="MKASRFFISTLKEAPADAEVASHKLMMRAGFIKRLGAGIYTYMP
                     ICLRVIRKVENIIREEMNRAGAVELLMPVVQPAELWQETGRFQTNGPELMRVKDRHDR
                     DFVIQPTSEEVITDIARQELRSYRTLPRNFYHIQTKFRDERRPRFGVMRGREFTMKDA
                     YSFDRDIESASKSYDIMFNAYTRIFERMGLTFRAVIADNGSIGGVRSHEFQVIADTGE
                     DAIIYCPTSDYAANIEMAEAVAPSAPRGAPAAPLTKTPTPGKSTCEDVAELLGVPLQR
                     TVKSLVLATDETSAAGDVVKTTVWLLLVRGDHALNEIKASKVEGLDKGFRFATVAEIE
                     THFGCKPGYLGPIGRRGSEQRSFKVVADRTVAQMSDFICGANDADFHYTGVNWGRDLP
                     EPDLVADIRNVVEGDPSPDGKGRLAIQRGIEVGHVFLLGAYYSEKMNATYLDETGKPQ
                     LMQMGCYGIGVTRLLGAAIEQNFDARGIVWPAAIAPFSVVICPIGYDRSADVKAAADR
                     LHEELQAAGIDVMLDDRGERPGAMFADWELIGVPQRVVLSDRGLKEGQAEVQGRRDAQ
                     ATAIAIANVLPTLKAKLGA"
     gene            1796..2437
                     /locus_tag="VNU48_01520"
     CDS             1796..2437
                     /locus_tag="VNU48_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWI09987.1"
                     /translation="MSGSLSRIGRARRRLVVAAPLAGLLPTPAWAGAQIEEPLANAVR
                     SALAAAVNDTAPPRPSFDTMNARLVYLRWLGEMSGRLRKRESEHLRRVEFLQTAWYES
                     TRAGLEPSLLLGLVQVESGFRKYAVSSAGARGYMQVMPFWARLIGDGDAARLFHMQTN
                     LRFGCVILRHYLDLERGDLYLALGRYNGSRGRPEYPDAVFASQRQWLYKPPLA"
     gene            complement(2442..3017)
                     /locus_tag="VNU48_01525"
     CDS             complement(2442..3017)
                     /locus_tag="VNU48_01525"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HWI09988.1"
                     /translation="MTATPAARVVVGVTGATGAVYALRVLQRLKAAGAQTHLVASPAG
                     VVNAHHELGLDRHALEALADHAYNPADIGAAIASGSFQIDAMVVAPCSMRTLAAIAHG
                     LSDNLLTRAADVMLKERRRLVLMVRETPFNLAHLRNMTTVTEMGGVIFPPLPAFYHRP
                     QSIDEMVADTVERVLALIGIEGAAPKTWTGL"
     gene            complement(3014..>3214)
                     /locus_tag="VNU48_01530"
     CDS             complement(3014..>3214)
                     /locus_tag="VNU48_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003271501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HWI09989.1"
                     /translation="QVLKACGATDITTVNVYDDEEIRQGIKDYAQWPTIPQLYVNGEF
                     VGGSDIMMEMYQAGELQPLLSA"
BASE COUNT          528 a         1102 c         1084 g          500 t
ORIGIN      
        1 accggtgccg cgctcagccg cacacccaca cgcgcccttc catgaaagca tcccggttct
       61 tcatctccac gctcaaagaa gcgcctgccg acgccgaagt cgcgagccac aaattaatga
      121 tgcgcgccgg cttcatcaag cggctcggcg ccggcatcta tacctacatg ccgatctgcc
      181 tgcgcgtgat tcgcaaggtc gagaacatca ttcgggaaga gatgaaccgc gccggcgccg
      241 tcgagttgct gatgccggtg gtgcagccgg ccgaactctg gcaggagacg ggccgcttcc
      301 agaccaacgg ccccgagttg atgcgcgtga aggatcgcca cgaccgcgac ttcgtgatcc
      361 agccgaccag cgaagaggtc attaccgaca tcgcgcggca ggagctgcgc agctaccgca
      421 cgctgccgcg aaatttctat cacatccaga ccaagttccg cgacgagcgc cggccgcgct
      481 tcggcgtgat gcgcgggcgc gagttcacga tgaaggacgc ctactcgttc gaccgcgaca
      541 tcgagtcggc gagcaagagc tacgacatca tgttcaatgc ctacacgcgc atcttcgagc
      601 gcatgggctt gaccttccgc gcggtgatcg ccgacaacgg ctcgatcggc ggcgtgcgct
      661 cgcacgagtt ccaggtcatc gccgacaccg gcgaagacgc gatcatctat tgcccgacat
      721 cggactacgc ggccaacatc gagatggccg aggcggtggc gccgagcgcg ccgcgcggcg
      781 cgcctgcggc gccactgacg aagacgccga cccccggcaa gagcacctgt gaagacgtcg
      841 ccgaactgct cggcgtgccc ttgcaacgca ccgtcaagtc gctggtgctc gcgaccgacg
      901 agaccagcgc cgccggcgac gtcgtcaaga ccaccgtgtg gctgctgctg gtgcgcggcg
      961 atcatgcgct gaacgagatc aaggcgagca aggtcgaggg tctggacaag ggcttccggt
     1021 tcgccacggt ggccgaaatc gaaacccact tcggctgcaa gccgggctac ctcggcccga
     1081 tcggccggcg tggatcagag cagcggtcgt tcaaggtggt cgccgatcgc accgtcgcgc
     1141 agatgagcga cttcatctgc ggcgcgaacg atgccgactt ccactacacc ggggtcaact
     1201 ggggccgcga cctgcccgag cccgacctcg tcgccgacat ccgcaatgtc gtcgagggcg
     1261 atccgtcgcc cgacggcaag gggcgcctgg cgatccagcg cggcatcgag gtcggccatg
     1321 tgttcctgct cggcgcgtac tacagcgaga agatgaacgc gacctacctc gacgagaccg
     1381 gcaagccgca gctgatgcag atgggctgct acggcatcgg cgtgacgcgc ctgctcggcg
     1441 ccgcgatcga gcaaaacttc gacgcgcgcg gcatcgtctg gccggccgcg atcgcgccgt
     1501 tcagcgtcgt gatctgcccg atcggctacg accggtccgc cgacgtgaag gccgccgccg
     1561 accgcctgca cgaggagttg caggcggccg gtatcgacgt gatgctcgac gaccgcggcg
     1621 aacgccccgg cgcgatgttc gccgactggg agctgatcgg cgtgccgcag cgtgtcgtgc
     1681 tgtccgatcg cgggctcaag gaaggtcagg ccgaagttca gggccggcgc gacgcgcagg
     1741 cgaccgcgat cgcgatcgcc aatgtgctgc cgacgctgaa ggccaagctc ggcgcatgag
     1801 cgggtctctc tcccgcatcg ggagagcgcg gcgccgtctt gtcgtcgcgg ccccgctggc
     1861 cggcttgctg cccacaccgg catgggccgg cgcgcagatc gaggagccgc ttgccaacgc
     1921 cgtgcgatcg gcactggcgg cggcggtcaa cgacacggcg ccgccgcggc cgtcgttcga
     1981 tacgatgaac gcgcgcctgg tttacctgcg ctggctcggc gagatgagcg gccggctgcg
     2041 caagcgcgaa tccgagcacc tgcgacgcgt cgaattcctc cagaccgcgt ggtacgagag
     2101 cacgcgcgcc ggcctcgagc cgtcgctgct gctcgggctg gtccaggtcg agagcggctt
     2161 tcgcaagtac gcggtcagca gcgccggcgc gcgcggctac atgcaggtga tgccgttctg
     2221 ggcgcgcctg atcggcgacg gcgatgcggc gcggctgttc cacatgcaga ccaacctgcg
     2281 cttcggctgc gtgatcctgc gccactacct cgatctggag cgcggtgacc tgtacctggc
     2341 gctgggccgc tacaacggca gccgcggtcg ccccgaatac ccggacgcgg tgttcgcgtc
     2401 gcagcgccag tggctctaca agccgccgct ggcctgatcg gctacaggcc cgtccaggtc
     2461 ttgggcgcgg cgccctcgat gccgatcagc gcgagcacgc gctcgaccgt gtcggccacc
     2521 atctcgtcga tgctctgcgg gcggtggtag aacgccggca gcggcgggaa gatcacgccg
     2581 cccatctcgg tcacggtcgt catgttgcgc aggtgggcga ggttgaacgg cgtctcgcgc
     2641 accatcagca cgaggcggcg ccgctccttc agcatcacgt cggccgcgcg cgtcagcagg
     2701 ttgtcggaca ggccgtgggc gattgccgcc agcgtgcgca tcgagcacgg cgcgacgacc
     2761 atcgcgtcga tctggaacga accgctggcg atcgccgcgc cgatgtccgc ggggttgtac
     2821 gcgtggtcgg cgagcgcctc cagcgcgtgg cgatccaggc cgagttcgtg atgggcgttg
     2881 accacgccgg ccgggcttgc gaccagatgc gtctgcgccc ctgccgcctt cagccgctgc
     2941 agcacacgca atgcgtagac cgcacccgtg gcgcccgtga cgccgacgac gacgcgcgcg
     3001 gcgggcgttg ctgtcaagcg ctcagcagtg gttgcagctc gccggcctgg tacatctcca
     3061 tcatgatgtc cgagccgccg acgaactcac cgttgacgta gagctgcggg atcgtcggcc
     3121 actgcgcgta gtccttgatg ccttggcgaa tttcttcgtc gtcgtacacg ttgacggtgg
     3181 tgatgtcggt cgcaccgcag gccttcagca cctg
//
LOCUS       DATMJB010000064         2168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_286731, whole genome shotgun sequence.
ACCESSION   DATMJB010000064 DATMJB010000000
VERSION     DATMJB010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286731"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1033)
                     /locus_tag="VNU48_01535"
     CDS             complement(245..1033)
                     /locus_tag="VNU48_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HWI09990.1"
                     /translation="MRNQPQESGMASLNRLAASLAAALALSAWFGTTPSSAATSCSAR
                     SGAVAPAVVELYTSEGCSSCPPADRWLSRLKNEPGVVALAFHVDYWDRLGWKDRFASP
                     DYTQRQAQQQGVSGARFSYTPQVIVNGIDSPDWPRASIAAAAARPSPVDVQLSRDGTR
                     VSAVVAPLKGAPARLAAYWAVTEQNHVSAVKAGENEGATLTHDDVVREYRRVPAWAVT
                     PDGPHATLQFELGAAADPAHPRQVNLVIIDAASGRPVQAVGLGC"
     gene            1109..>2168
                     /locus_tag="VNU48_01540"
     CDS             1109..>2168
                     /locus_tag="VNU48_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HWI09991.1"
                     /translation="MHSSPKRRVAVIGGGPAGLMAAECLAAGGVQVELFDAMPSVGRK
                     FLLAGKGGLNLTHSEPLAAFLGRFGARRAVLEPMLRAFGPAELRAWAQGLGVTTFVGS
                     SGRVFPSDLKAAPLLRAWLHRLRAAGVVFHMRQRWLGWCEGGSELRFAGPQGEHDVSA
                     DALVLALGGGSWPRLGSDGAWLRLLAARGVDVAPLQASNCGFEVARTGEGGALTAGWS
                     GFFRGKFAGSPMKNVALALDAHDGRTIAQRGEFVVTEHGVEGSLVYALSGPARDTIAA
                     RGHALLRIDLLPARSIEFVRDEVARPRGSRSLATHLKSRLGLDGVKAALLHELLPREA
                     IADPARLAAAIKALPLTLV"
BASE COUNT          274 a          769 c          806 g          319 t
ORIGIN      
        1 aacgcagcga agtaaaggag gagggacggg cgcagcccgt tccgggggac atgagcggag
       61 ttgtccaccc gactctctcg gctcgcgccg accgtcaaag ggcgcacgct cgcttgcgaa
      121 cgaccgcaac gggccgcaag cagacatccc aagccccagc ggcgtttcat gcggtgtgaa
      181 cgacccacca tgcccgctcg ggcacgccgg attcattcac tttcacaccg tctgaccgga
      241 gagttcagca gccgagcccg acggcctgca ccggccggcc gctggcggcg tcgatgatca
      301 ccaggttgac ctggcgcgga tgcgccgggt cggccgccgc gccgagctcg aactgcaggg
      361 tcgcgtgcgg gccgtcgggc gtgaccgccc aggcgggcac gcggcggtac tcgcgcacca
      421 cgtcgtcgtg ggtcaaggtc gcgccttcgt tctcgcccgc cttcaccgcg ctcacgtggt
      481 tctgctcggt gacggcccag tacgcggcca gccgtgccgg tgcacccttc agcggcgcga
      541 cgacggcgct cacgcgcgtg ccgtcgcggc tgagctgcac atcgaccggc gacggccgcg
      601 ccgccgcggc cgcgatcgat gcacgcggcc agtcgggact gtcgatgccg ttgacgatca
      661 cctgcggcgt gtagctgaag cgggcaccgc tgacgccctg ctgctgcgcc tggcgctgcg
      721 tgtagtcggg gctcgcgaag cggtctttcc agcccaggcg atcccagtag tcgacgtgga
      781 acgcgagcgc gacgacaccg ggctcgttct tcagacgcga gagccagcgg tcggccggcg
      841 ggcacgagct gcagccttcg gaggtgtaga gctcgaccac cgccggcgcc accgcgccgc
      901 tgcgcgcgct gcagctcgtc gccgcgctgc tcggggtcgt gccgaaccag gccgacagcg
      961 cgagcgcggc ggccagcgat gcagcaagac ggttgagcga ggccatgccg gactcctggg
     1021 gttggttgcg catcggtcgc gccgtggacg cggttcttac ggcatcgtgt gccacactgc
     1081 gccgccgtcg aacccgtccg ttgccgccat gcattcttcc ccaaagcgcc gcgtcgccgt
     1141 gatcggcggc ggcccggccg gcctgatggc cgccgagtgc ctcgcggccg gcggcgtgca
     1201 ggtcgagctg ttcgacgcga tgccgtcggt gggccgcaag ttcctgctcg ccggcaaggg
     1261 cgggctgaac ctgacccaca gcgagccgct agctgcgttc ctgggccgct tcggcgcgcg
     1321 ccgcgcggtg ctcgagccga tgctgcgcgc gttcggcccg gccgagctgc gcgcctgggc
     1381 gcagggcctg ggggtgacga ccttcgtcgg cagctcgggc cgcgtcttcc cgagcgacct
     1441 gaaagcggcg ccgctgctgc gcgcctggct gcaccggctg cgcgcagccg gcgtcgtgtt
     1501 ccacatgcgg cagcgctggc tgggctggtg cgagggcggc agcgagctgc gtttcgccgg
     1561 tccacagggc gagcatgacg tgtccgccga tgcgctggtg ctcgcgctcg gcggtggcag
     1621 ctggccccgg ctcggctccg acggtgcgtg gttgcggctg ctggcggcgc gtggcgtcga
     1681 cgtggcgccg ctgcaggcgt cgaactgcgg cttcgaagtc gcgcgcaccg gcgagggcgg
     1741 ggcgctgaca gcaggctgga gcggcttctt ccgcggcaag ttcgcgggct ctccgatgaa
     1801 gaacgtcgcg ctcgcgctgg acgcgcacga cggtcgcacg atcgcgcagc gcggcgagtt
     1861 cgtcgtcacc gaacacgggg tcgaaggcag cctggtctac gcgctgtcgg gcccggcacg
     1921 cgacaccatc gcggcgcgcg gtcatgcgct gctgcggatc gacctgctgc cggcccgctc
     1981 gatcgaattc gtccgcgacg aggtggcccg gccgcgcggt tcgcgttcgc tcgcgacgca
     2041 cctgaagagc cggctcggcc tcgacggggt caaggccgcg ctgcttcacg aactgctgcc
     2101 gcgtgaggcg atcgccgacc cggcacggct cgcggcggcg atcaaggcgc tgccgttgac
     2161 gctggtcg
//
LOCUS       DATMJB010000065         2123 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_582407, whole genome shotgun sequence.
ACCESSION   DATMJB010000065 DATMJB010000000
VERSION     DATMJB010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582407"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1640)
                     /gene="sbcB"
                     /locus_tag="VNU48_01545"
     CDS             complement(207..1640)
                     /gene="sbcB"
                     /locus_tag="VNU48_01545"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HWI09992.1"
                     /translation="MADMTFFWHDYETFGRVPRRDRPSQFAGVRTDADLNEIDAPLMQ
                     YCRPAPDFLPEPEACVLTGITPQRCLEFGVPEHAFADAIEQQLARPGTIGVGYNSIRF
                     DDEVTRFLFWRNLIDPYAREWQDECGRWDLLDVVRCTWALRPEGIEWPLHEDGKPSFK
                     LEHLTAANGLAHAAAHDALSDVRATIALARLIKMRQPRLWEFCLRLRSKHEVVREMGV
                     GRPFLHISGMYGTERGCLAPVWPLAPHPSNKNEVIVWDLSVDPAELFTLGVAEIRTRM
                     FTRADELPEGVARLPIKTIHINKSPIVIGNLKTLSAERAAQWGLDIPQALRHAELVAH
                     KANLLAGMWPAVFERPAREGPTDVDEDLYGGFVGNEDRRMLQRLRALAGAELAAKRPA
                     FGDARLGELLFRYRARNFPDTLSEPEQAQWREHCAHRLHDGAGGGLTLEAFLERVDAL
                     GEAADERGQEILGALVDYATQIAPERH"
     gene            1715..>2123
                     /locus_tag="VNU48_01550"
     CDS             1715..>2123
                     /locus_tag="VNU48_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWI09993.1"
                     /translation="MTRVFNFSAGPAALPESVLRQAADEMLDWHGSGMSVMEMSHRGK
                     EFISIYEEAERDFEAWWEKWARELHWFEPWQTTLEWDAPWAKWFQEGKLNASHNCLDR
                     HVEAGNGDRIAYYWEGEDGKRAEITYAELLDMTC"
BASE COUNT          322 a          711 c          768 g          322 t
ORIGIN      
        1 atccagggtc gccattccct gcgggccgcg cgggctcatg ttgctgcagg cagcaagtgt
       61 ggacacgagg acgaaggggg ccagggcgag tcggatgttc atcaaagctc caggtcatcg
      121 aaaagggctt ggaaaatgcc gggccattat gcccagcgcg ccgtcagggg aacggggaac
      181 gggccgctgt cggtgtgcga cgcgggtcag tggcgctccg gcgcgatctg ggtggcgtag
      241 tcgaccagcg cgccgaggat ctcctggccg cgttcgtcgg ccgcttcgcc gagggcgtcg
      301 acccgctcga ggaacgcttc cagcgtcaac ccgcccccgg cgccgtcgtg cagccggtgc
      361 gcgcaatgct cgcgccactg cgcctgctcg ggctcgctca gcgtgtcggg aaagttgcgg
      421 gcgcggtagc ggaacagcag ctcgcccagg cgcgcgtcgc cgaaggccgg gcgcttggcg
      481 gcgagttctg cgccagctag cgcacgcagg cgctgcagca tgcgccggtc ctcgttgccg
      541 acgaagccgc cgtacaggtc ttcgtcgaca tcggtcgggc cttcgcgcgc cggccgctcg
      601 aacaccgcgg gccacatgcc ggccagcagg ttcgccttgt gcgcgacgag ttccgcgtgg
      661 cgcagcgcct gcgggatgtc caggccccac tgcgcggccc gctcggcgct gagcgtcttc
      721 aggttgccga tcacgatcgg cgacttgttg atgtggatgg tcttgatcgg cagccgcgcc
      781 acgccctcgg gcagctcgtc ggcgcgggtg aacatgcggg tgcggatctc ggccacgccg
      841 agcgtgaaca gctcggccgg gtcgaccgac aggtcccaga cgatcacctc gttcttgttg
      901 ctcgggtgcg gcgccagcgg ccacaccggc gcgaggcaac cgcgctcggt gccgtacatg
      961 ccggagatgt gcagaaaggg tctgccgacg cccatctcgc gcacgacctc gtgcttgctg
     1021 cgcagccgca ggcagaactc ccacagccgc ggctggcgca tcttgatcag ccgcgccagc
     1081 gcgatcgtcg cgcgcacgtc ggacagcgcg tcgtgcgccg cggcatgcgc gaggccgttc
     1141 gccgcggtca ggtgctcgag cttgaacgag ggcttgccgt cctcgtgcag cggccattcg
     1201 atgccctcgg ggcgcagcgc ccaggtgcag cgcaccacat cgagcaggtc ccagcggccg
     1261 cactcgtcct gccactcgcg cgcgtacggg tcgatcaggt tgcgccagaa caggaagcgc
     1321 gtgacctcgt cgtcgaagcg gatcgagttg tagccgacgc cgatcgtgcc gggccgcgcg
     1381 agctgctgct cgatcgcatc ggcgaacgcg tgctcgggca cgccgaactc caggcagcgc
     1441 tgcggcgtga tgccggtcag cacgcaggcc tcgggttcgg gcaggaagtc gggcgcgggc
     1501 cggcagtact gcatcagcgg ggcgtcgatc tcgttcaggt cggcatcggt gcggaccccg
     1561 gcgaactgcg acgggcggtc gcgccgcggc acgcggccga aggtttcgta gtcgtgccag
     1621 aagaaggtca tgtcggccat ccagagaatc taacaggggg ccggcttcgg ctaagcttgc
     1681 gctccccttc cctgccgctc ggagacccga tcccatgacc cgtgttttca acttcagcgc
     1741 cggcccggcc gcgctgcccg aatccgtgct gcgtcaggcc gcggacgaga tgctcgactg
     1801 gcacggctcg ggcatgtcgg tgatggagat gagccaccgc ggcaaggagt tcatctccat
     1861 ctacgaggag gccgagcgcg acttcgaggc ctggtgggag aagtgggcgc gcgagctgca
     1921 ctggttcgag ccctggcaga cgacgctcga gtgggacgcg ccgtgggcga agtggttcca
     1981 ggaggggaag ctgaacgcgt cgcacaactg cctcgaccgc cacgtcgagg cgggcaacgg
     2041 cgaccgcatc gcctactact gggagggcga ggacggcaag cgcgccgaga tcacctacgc
     2101 cgagctgctc gacatgacgt gcc
//
LOCUS       DATMJB010000066         6414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_700654, whole genome shotgun sequence.
ACCESSION   DATMJB010000066 DATMJB010000000
VERSION     DATMJB010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6414
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700654"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /gene="recA"
                     /locus_tag="VNU48_01555"
     CDS             complement(<1..290)
                     /gene="recA"
                     /locus_tag="VNU48_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857918.1"
                     /note="catalyzes the hydrolysis of ATP in the presence of
                     single-stranded DNA, the ATP-dependent uptake of
                     single-stranded DNA by duplex DNA, and the ATP-dependent
                     hybridization of homologous single-stranded DNAs; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWI09994.1"
                     /translation="MDAPVKTLNTEKAKALQAALAQIEKQYGKGTIMRLGVGEVIDDI
                     EVVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQVVAQIQKLGGTA"
     gene            471..1142
                     /locus_tag="VNU48_01560"
     CDS             471..1142
                     /locus_tag="VNU48_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI09995.1"
                     /translation="MRILIAEDDQVLADGLLRSLRNAGYAVDQVGSGTEADAALASHE
                     FDMVILDLGLPKLHGLEVLRKLRARGASVPVLILTAADSVEQRVKGLDLGADDYMAKP
                     FSLQELEARVRALTRRGLGSASAIIRHGPLSFDATGRVAYLNDQMVELSARELSLLEV
                     LLQRAGRLVSKDQLVERLCEWGEEVSNNAIEVYIHRLRKKIEQGPVRIATVRGLGYCL
                     EKIPA"
     gene            1214..2746
                     /locus_tag="VNU48_01565"
     CDS             1214..2746
                     /locus_tag="VNU48_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HWI09996.1"
                     /translation="MLAPLLLLWPMSVVLTWLVAQGIANKPFDRELGEMVRVLAKEVS
                     VSAGAGRAQAVFKLPAAAAEILRTDEADDIYYQVLGLRGEYLSGDTALPVPGDEDRAP
                     PGEVRYRDETLNNAPVRVAYLWVNVQERVQERVPARDGAATAPVDAPLVQVAETLGKR
                     SRLATEIIKGVILPQFVILPLAVLLVWLALARGIAPLNELQQRIRKRDANDLSPIDER
                     QAPEEVSPLVRAINDLLARLDQSLRAQKHFLADAAHQLKTPLAGLRTQAELAQRQIDA
                     GQHDPQALKKSLQQIARSSQSAAHMVNQLLAMARAENQQQVAQRQVVNLARLATETVR
                     DFVPRALEKRIDLGYEGPGDAVVRRHPQGPQVLGHALLLRELIRNLVDNALQYTPAGG
                     TVTVRVIDDPFGQVVVLQVEDSGPGIAPAERELVFQPFYRSLGTEVDGSGLGLAIVRE
                     IATQHAAEVTVTDASTRSPPGTLITVRLPASPVTEAEALSSGPAADSSRSTSGSPGPA
                     RA"
     gene            complement(2679..3833)
                     /gene="rodA"
                     /locus_tag="VNU48_01570"
     CDS             complement(2679..3833)
                     /gene="rodA"
                     /locus_tag="VNU48_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HWI09997.1"
                     /translation="MSAVFERASPWQRIKPVFSGFDGPLLLAMLLLGAAGLVTMYSAG
                     FDAGTRFVDHGRNMLLALGVMFIVAQVSPHTLMKLAVPLYTVGVALLVAVAVMGVTKK
                     GATRWLNVGVVIQPSEILKIAMPLMLAWWFQRREGQLRVTDFVFALLLLLAPVGLIVK
                     QPDLGTAILVLSAGLYVIFFAGLSWKLIIPVLVIGAVAITAIVVSEDHICQPEVKWPM
                     MRDYQKNRVCTLLDPTTDPLGKGFHIIQGMIAIGSGGVDGKGFMKGTQTHLEFIPERT
                     TDFIFAAFSEEFGLIGCVGLVLGFLFLIYRGLMIAAEAPTAFTRLLAGAISLSWFTYA
                     FVNMGMVSGILPVVGVPLPFISYGGTAMVTLGLGLGILMSISKSRRLVQS"
     gene            complement(3833..5878)
                     /gene="mrdA"
                     /locus_tag="VNU48_01575"
     CDS             complement(3833..5878)
                     /gene="mrdA"
                     /locus_tag="VNU48_01575"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWI09998.1"
                     /translation="MTELKNVEQEVGRFRMRLAAAAAFVLFAFALLAARLVYLQVYKH
                     DELSTQAENNRITVVPIVPNRGLILDRNGVVLANNYSAYTLEVTPGKVDDLEATIDRL
                     AGVVTIEARDRKRFKRLMEENKSFESLPIRTKLTDEEVARFTAQRFLFSGVDIKARLF
                     RNYPLGQVGSHLIGYIGRINEAEKDKIDDSEDEANYRGTQYIGKLGVEQSYEAELHGT
                     VGFEEMETSASGHPVRRLDSKPATPGNALVMSIDIRLQALVEELFGERRGALVAIDPR
                     NGEVLAFVSKPNFDPNLFVDGIDSDNWKALNESPDKPLLNRALRGTYSPGSTYKPFMA
                     LAALTLGKRTPQQTIYDPGSYTFGGHTFRDDKEGGHGTVDMYKSIVQSCDTYYYMLAN
                     DLGVDAIHDFMAPLGLGQLTGIDMQGELRGVLPSTEWKRNAYRKKELQKWYAGETISL
                     GIGQGQNTFTMLQMAQATAALASGGQRFRPHLVKLVENFETREQRRQIGEALPPMDWK
                     PEHVAFIRHAMYGVTQEGTARGAFLNAPYQAGGKTGTAQVIAIKQGEKYNARNLDERH
                     RDNALFTAFAPVDAPQIAIALVVENAGFGAGAAAPIARRVIDYVLTGMYPSEADIAAT
                     QLGQSTAPIGVPRPAASVPLPGTTVDGGAAAALLTPAPAPAAAAASAALHVALDNKP"
     gene            complement(5875..6396)
                     /gene="mreD"
                     /locus_tag="VNU48_01580"
     CDS             complement(5875..6396)
                     /gene="mreD"
                     /locus_tag="VNU48_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HWI09999.1"
                     /translation="MIMPRANGQLLLPVNPLFVAATLLIAFALNIVPLGRVPLMPDFV
                     ALVLVFWNVHQSRRVGVGLAFFFGVLMDVHDGAVLGQHALAYTLMSYFAVTIHRRLLW
                     FTVPSQAVQILPLFLAAHAVSLVVRMIAGGMFPGWQVLLAPAFEALLWPIATWLLLAP
                     QRRAPDPDQNRPL"
BASE COUNT         1022 a         2203 c         2226 g          963 t
ORIGIN      
        1 gccgtgccgc ccagtttctg gatctgtgcg accacctgca gcgtgagcgt ggtcttgccc
       61 gaggattcgg gaccgtagat ctcgacgacg cggccgcgcg gcaggccgcc gacgccgagc
      121 gcgatgtcga ggccgagcga gccggtcgag acgacctcga tgtcgtcgat cacctcgccg
      181 acgccgagcc gcatgatcgt gcccttgccg tactgcttct cgatctgcgc gagggcggcc
      241 tgcagggcct tggctttttc ggtgttcagg gtcttgaccg gggcatccat aacgtttctc
      301 ctgggaaggt gggcgcatta tcgcaaaagc tggatgtttg tacagtgatg cgaaggcatt
      361 cttattgggg tatctcgcga cgattcgtgc ggcccggccg acggcccgaa tcctagaatc
      421 gcagcagcgc ccgcaaggca agaccaacag tggcgccacg gagacaagcc atgcgcatcc
      481 tgatcgccga agatgatcaa gtgctggccg atggcttgct gcgctcgctg cgcaatgcgg
      541 gctacgcggt cgatcaggtc ggcagcggca ccgaggccga cgcggcgctg gcctcgcacg
      601 aattcgacat ggtgatcctc gacctcggcc tgcccaagct gcacgggctg gaggtgctgc
      661 gcaagctgcg cgcgcgcggc gcgagcgtgc cggtgctgat cctgacggcc gccgacagcg
      721 tcgagcagcg cgtcaagggg ctggacctcg gtgccgacga ctacatggcc aagcccttct
      781 cgctgcagga actggaggcg cgcgtgcgcg cgctcacgcg gcgcggcctg ggctcggcct
      841 cggcgatcat ccgccacggc ccgctgagct tcgacgcgac ggggcgcgtc gcctacctga
      901 acgaccagat ggtcgagctc tcggcgcgcg agctgagcct gcttgaggtg ctgctgcagc
      961 gcgccgggcg gctggtcagc aaggaccagc tggtcgagcg cctgtgcgaa tggggcgagg
     1021 aggtcagcaa caacgcgatc gaggtctaca tccaccgcct gcgcaagaag atcgagcagg
     1081 ggccggtgcg catcgcgacc gtgcgcggcc tgggctactg cctcgagaag atccccgctt
     1141 gaggccggcc gtggccgaag ccgagccacg tccacgcgaa cagcgctcgc tgttcggcga
     1201 gatcctcgac tggatgctcg cgccgctgct gctgctgtgg ccgatgagcg tggtgctgac
     1261 ctggctggtc gcgcagggca tcgccaacaa gcccttcgac cgcgagctcg gcgagatggt
     1321 gcgggtgctg gcgaaggagg tctcggtcag cgccggcgcc gggcgcgccc aggccgtgtt
     1381 caagctgccg gcggcggcgg ccgagatcct gcgcaccgac gaggccgacg acatctacta
     1441 ccaggtgctc gggctgcgcg gcgaatacct cagcggcgac acggcgctgc cggtgcccgg
     1501 cgacgaggac cgcgcaccgc cgggcgaggt gcgctaccgc gacgagacgc tgaacaacgc
     1561 accggtgcgc gtggcctacc tgtgggtgaa tgtgcaagag cgggtgcaag agcgcgtgcc
     1621 cgcacgcgac ggcgccgcga cggcaccggt cgacgcgccg ctggtgcagg tcgccgagac
     1681 gctgggcaag cgctcgcgtc tggccaccga gatcatcaag ggcgtgatcc tgccgcagtt
     1741 cgtgatcctg ccgcttgcgg tgttgctggt gtggctggcg ctggcgcgcg gcatcgcgcc
     1801 gctgaacgag ttgcagcagc gcatccgcaa gcgcgacgcc aatgacctga gcccgatcga
     1861 cgaacgccag gcgcccgaag aggtctcgcc gctggtgcgc gcgatcaacg acctgctcgc
     1921 gcggctggac cagtcgctgc gcgcgcagaa gcacttcctg gccgacgccg cgcaccagct
     1981 gaagacaccg ctggccgggc tgcgcaccca ggccgagctg gcgcagcgcc agatcgacgc
     2041 cggccagcac gacccgcagg cgctgaagaa atcgctgcag cagatcgcac gctcgagcca
     2101 gagcgcggcc cacatggtga accagctgct cgcgatggcg cgggccgaga accagcagca
     2161 ggtcgcgcag cgacaggtcg tcaacctggc gcgccttgcg accgagacgg tgcgcgactt
     2221 cgtgccgcgc gcgctcgaga agcgcatcga tctcggctac gaggggccgg gcgacgcggt
     2281 ggtgcggcgc cacccgcaag gcccgcaggt gctcggccac gcgctgctgc tgcgcgagct
     2341 gatccgcaac ctcgtcgaca acgccttgca gtacacgccg gccggcggca cggtcacggt
     2401 gcgcgtgatc gacgacccgt tcggccaggt cgtcgtgctg caggtcgagg actccgggcc
     2461 gggcatcgcg ccggccgagc gcgagctggt gttccagccc ttctaccgct cgctgggcac
     2521 cgaggtcgac ggctcgggcc tggggctggc gatcgtgcgc gagatcgcga cgcagcacgc
     2581 ggccgaagtc acggtcaccg acgcgtcgac gcgcagcccg ccgggcacgc tgatcacggt
     2641 gcgcctgccg gccagccccg tgaccgaggc cgaggcgctc agctctggac cagccgccga
     2701 ctcttcgaga tcgacatcag gatccccagg cccagcccga gcgtgaccat cgcggtgccg
     2761 ccgtagctga tgaacggcag gggcacgccc accaccggca ggatgccgct gaccatgccc
     2821 atgttgacga acgcgtaggt gaaccagctc agcgagatcg caccggccag cagccgcgtg
     2881 aacgcggtcg gcgcctcggc cgcgatcatc aggccgcggt agatcaggaa caggaagccc
     2941 agcaccagcc cgacgcagcc gatcaggccg aactcttcgg agaacgccgc gaagatgaaa
     3001 tcggtggtgc gctcggggat aaactcgagg tgcgtctgcg tgcccttcat gaagcccttg
     3061 ccgtcgacgc cgccggagcc gatcgcgatc atcccctgga tgatgtggaa gcccttgccg
     3121 agcgggtcgg tggtcgggtc gagcagcgtg cagacgcggt tcttctggta gtcgcgcatc
     3181 atcggccact tgacctcggg ctggcagatg tggtcctccg agacgacgat cgccgtgatc
     3241 gccaccgcgc cgatcaccag caccggaatg atcagcttcc acgacagccc ggcgaagaag
     3301 atcacgtaca gcccggccga cagcaccagg atcgccgtgc ccagatcggg ctgcttgacg
     3361 atcaacccga ccggcgcgag cagcagcagc agcgcgaaga cgaagtcggt cacgcgcagc
     3421 tggccctcgc gacgctggaa ccaccacgcc agcatcagcg gcatcgcgat cttcaggatc
     3481 tcgctgggct ggatcaccac gccgacgttg agccagcgcg tcgcgccctt cttcgtcacg
     3541 cccatcaccg ccaccgccac cagcagcgcc acgcccaccg tgtacagcgg caccgcgagc
     3601 ttcatcagcg tgtgcggcga gacctgcgcg acgatgaaca tcacgcccag cgccagcagc
     3661 atgttgcggc cgtgatcgac gaagcgcgtg ccggcgtcga agccggccga gtacatcgtc
     3721 acgaggccgg ccgcgccgag cagcagcatc gcgagcagca gcgggccgtc gaagccgctg
     3781 aacaccggct tgatgcgctg ccagggcgac gcccgttcga agaccgcact catcagggct
     3841 tgttgtccag cgcgacgtgc agcgccgccg aggcggccgc ggccgccggc gcgggcgccg
     3901 gggtcagcag tgcggcagcc gcgccgccgt ccaccgtcgt gccgggcagc ggcaccgacg
     3961 ccgccggccg cggcacgccg atcggtgcgg tcgactggcc gagctgcgtc gccgcgatgt
     4021 cggcctcgct cgggtacatg ccggtgagca cgtagtcgat cacgcggcgc gcgatcggtg
     4081 ccgcggcgcc ggcgccgaag ccggcgttct cgaccaccag cgcgatcgcg atctgcggcg
     4141 catcgaccgg cgcgaacgcg gtgaacagcg cgttgtcgcg gtgccgctcg tcgagattgc
     4201 gcgcgttgta cttctcaccc tgtttgatgg cgatcacctg cgcggtgccg gtcttgccgc
     4261 cggcctggta cggcgcgttc aggaacgcac cgcgcgccgt gccttcctgc gtcacgccgt
     4321 acatcgcgtg gcggatgaag gccacgtgtt cgggcttcca gtccatcggc ggcaacgcct
     4381 cgccgatctg ccggcgctgc tcgcgcgtct cgaagttctc gaccagcttg accaggtgcg
     4441 gccggaagcg ctggccgccc gacgcgagcg cggcggtggc ctgcgccatc tgcagcatcg
     4501 tgaaggtatt ctggccctgg ccgatgccca gcgagatcgt ctcgcccgcg taccacttct
     4561 gcagctcctt cttgcggtag gcgttgcgct tccactcggt cgacggcagc acgccgcgca
     4621 gctcgccctg catgtcgatg ccggtgagct ggcccagccc cagcggcgcc atgaagtcgt
     4681 ggatcgcgtc gacgcccagg tcgttggcga gcatgtagta gtaggtgtcg caggactgca
     4741 cgatcgactt gtacatgtcg accgtgccgt ggccgccttc cttgtcgtcg cggaaggtgt
     4801 gcccgccgaa ggtgtacgag cccgggtcgt agatcgtctg ctgcggcgtg cgcttgccca
     4861 gcgtcagcgc ggccagtgcc atgaacggct tgtaggtcga gccgggcgag taggtgccgc
     4921 gcagcgcgcg gttcagcagc ggcttgtcgg gcgactcgtt cagcgccttc cagttgtcgc
     4981 tgtcgatgcc gtcgacgaac aggttcgggt cgaagttggg cttgctgacg aacgcgagga
     5041 cctcgccgtt gcgcgggtcg atggcgacga gcgcgccacg ccgctcgccg aacagttctt
     5101 cgaccagcgc ctgcagccgg atgtcgatcg acatcaccag cgcgttgccg ggggtcgccg
     5161 gcttgctgtc gagccgccgc accgggtggc cgctggcgct ggtttccatc tcctcgaagc
     5221 cgaccgtgcc atgcagctcg gcctcgtagc tctgctcgac gccgagcttg ccgatgtact
     5281 gcgtgccgcg gtagttggcc tcgtcctcgg agtcgtcgat cttgtccttc tcggcctcgt
     5341 tgatgcggcc gatgtagccg atcaggtggc tgccgacctg gcccagcggg tagttgcgga
     5401 acagccgcgc cttgatgtcg acgccgctga acaggaagcg ctgcgcggtg aagcgcgcca
     5461 cttcctcgtc cgtcagcttg gtgcggatcg gcagcgactc gaagctcttg ttctcttcca
     5521 tcaggcgctt gaagcgcttg cggtcgcgcg cttcgatcgt gacgacgccg gccagccggt
     5581 cgatcgtcgc ctcgaggtcg tcgaccttgc ccggcgtgac ctcgagcgtg taggccgagt
     5641 agttgttggc cagcacgacg ccgttgcggt ccaggatcag cccgcggttc ggcacgatcg
     5701 gcacgaccgt gatgcggttg ttctcggcct gggtcgagag ctcgtcatgc ttgtagacct
     5761 gcaggtagac cagccgcgcc gcgagcaaag cgaacgcgaa cagcacgaac gcggccgcgg
     5821 ccgcgaggcg catgcgaaag cgcccgacct cctgttcgac gtttttcagc tcggtcacaa
     5881 agggcgattc tgatccggat cgggcgcgcg gcgttgcggc gcgagcagca accacgtcgc
     5941 gatcggccac agcagcgcct cgaacgccgg cgcgagcagc acctgccagc ccgggaacat
     6001 gccgccggcg atcatgcgca cgaccagcga gaccgcgtgc gcggccagga acaacggcag
     6061 aatctgcacc gcctgcgacg gcaccgtgaa ccacagcagg cggcggtgga tcgtcaccgc
     6121 gaagtagctc atcagcgtgt aggccagcgc gtgctggccg agcaccgcgc cgtcgtgcac
     6181 gtccatcagc acaccgaaga agaacgccag cccgacgccg acccggcgcg actggtgcac
     6241 gttccagaac accagcacca gcgcgacgaa gtccggcatc agcggcaccc gcccgagcgg
     6301 cacgatgttc agcgcgaacg cgatcagcag cgtcgccgcg acgaacagcg ggttgacggg
     6361 cagcagcagc tggccattgg cgcgcggcat gatcatgacg cgtgcccctg tggc
//
LOCUS       DATMJB010000067         3455 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_112035, whole genome shotgun sequence.
ACCESSION   DATMJB010000067 DATMJB010000000
VERSION     DATMJB010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112035"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VNU48_01585"
     CDS             <1..492
                     /locus_tag="VNU48_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWI10000.1"
                     /translation="SLVMTRDAIYEAFLDDDVARGFLHSHSYTGNALACRAALAVLDR
                     FEHDDVLRVNRQRALALTLALAPLKTDSRVEHFRQLGMIWAFDVREDVAGARFAERFH
                     LAGRDHELLIRPIGRTVYLLPPYVLDDALSYWLGERVLATLAAVTTAGGSHADPAPEP
                     ATA"
     gene            458..1675
                     /gene="bioF"
                     /locus_tag="VNU48_01590"
     CDS             458..1675
                     /gene="bioF"
                     /locus_tag="VNU48_01590"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HWI10001.1"
                     /translation="MLIQHLSRQLRERETQALRRRRRIVETPCAPHLSVSSDGADARP
                     MLAFCSNDYLGLAHHPVLAAALAEGALRWGAGSGASHLISGHSRAHAALEEELADFLE
                     PCIPRARALSFCTGYMANLALLAALGDARATIFADKLNHASLVDGALLAKAPLRRYAH
                     GRIDVLADRLARCETPIKLIVTDAVFSMDGDLAPLPELLALAERFDAWLVVDDAHGFG
                     VLGEQGRGTLSHFGLRSERFICMGTLGKAAGVAGAFVAADPTIIDWLVQAARPYIYTT
                     AAPPAMAHALRTSLQLIRGDEGAQRRVRLATLITLLRRRLGALIAATPQRGWTLAESA
                     TAIQPLIVGDNEAALALSAALESRGLWVPAIRPPTVPVGSARLRITLSASHTEDDVLR
                     LGDALEHAAKGLA"
     gene            1705..2430
                     /gene="bioD"
                     /locus_tag="VNU48_01595"
     CDS             1705..2430
                     /gene="bioD"
                     /locus_tag="VNU48_01595"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HWI10002.1"
                     /translation="MTGTDTEVGKTRISAALLHWVASRGRVAVGFKPVAAGTTLIGGM
                     RVNEDVRLLRDASSAGLTDAEVGPFQFDAACAPHVAAALEGRAIDRAAILAAGRALRA
                     RGDFLVVEGVGGFCVPLGPDWDSSDIACGLALPVILVVGLRLGCLNHALLTAEAIKAR
                     HLPLAGWIANTIDPAMAYLDHSVSTLRHEFDERRQLPCLGVVPWLDDPHPEAVAAHLD
                     DAGLTLALGLPERVAPVVTLDPP"
     gene            2427..>3455
                     /locus_tag="VNU48_01600"
     CDS             2427..>3455
                     /locus_tag="VNU48_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWI10003.1"
                     /translation="MTSTAAASSFRVAAPRPAPWGRPIVDAAFLADPYPTYAALREAG
                     PIHWSEEFFGGAWLLTRHADVEAVLRDAARFSAQRTGGWVMRSDVARRELRGFQQLFA
                     RAMLFVDAPDHARIRGVLNAGFRSDVLQRFAPAVERRVDALLDAVNATGADAGFDFMQ
                     ALARPLPADVMAMLMDIPAADRGDFIAWSDDLASFIGAPLPSHDRARRAQASLLAMSG
                     YFERLVAQRRDAPGDDLVSRLLRAETAGDIRSGAELLAQCAMLLFAGHETTRNLLGNG
                     LQALLAHPHQWQRLQREPGLLPGAVRELLRFESPVQYTGRRVATDLVLHGQQLRRGDL
                     VVALIGAAN"
BASE COUNT          428 a         1240 c         1244 g          543 t
ORIGIN      
        1 tcgctcgtga tgacacgcga cgccatctat gaagccttcc tcgacgacga cgtggcgcgg
       61 ggcttcctgc attcgcattc gtataccggc aacgcgctgg cctgtcgcgc cgcgctggcg
      121 gtgctcgatc gtttcgaaca cgacgacgtg ctgcgcgtca accggcagcg cgcccttgcg
      181 ctgaccctgg cgctggcgcc gctgaagacc gattcgcgtg tcgagcattt tcggcagctc
      241 ggcatgatct gggccttcga cgtgcgggaa gacgtggccg gggcgcgctt tgccgagcgc
      301 ttccacctcg ccggtcgcga ccacgagttg ctgatccgcc cgatcggccg caccgtctac
      361 ctgctgccgc cctacgtact cgacgacgcg ctgtcgtact ggctcggcga gcgtgtcctc
      421 gcaaccctgg ccgcggtcac gaccgccggc ggttcccatg ctgatccagc acctgagccg
      481 gcaactgcgt gagcgcgaga cgcaggcgct gcgccggcgc cgaaggatcg tggagacgcc
      541 gtgcgcgccg cacctgtcgg tgtcgagcga cggcgccgac gcgcgcccga tgctcgcgtt
      601 ctgcagcaac gactacctcg gcctggcgca ccatcccgtc ctcgccgcgg cgctggccga
      661 aggcgcgttg cgctggggcg ccggcagtgg ggcctcgcat ctgatcagcg gtcactcgcg
      721 cgcgcatgcg gcgctcgaag aggaactcgc cgacttcctc gagccctgca tcccgcgcgc
      781 gcgggcactg agtttctgca ccggctacat ggccaacctc gcgctgctcg cggcattggg
      841 cgacgcccgg gcgacgatct tcgccgacaa gctgaatcat gcctcgctgg tcgacggcgc
      901 cttgctggcc aaggcgccgc tgcggcgcta cgcgcatggc cggatcgatg tgctggcgga
      961 ccggctcgcg cgctgcgaaa cgccgatcaa gctgatcgtg accgatgcgg tgttcagcat
     1021 ggacggcgat ctggcgccgc tgcccgagct gctcgcgctg gccgagcgct tcgacgcgtg
     1081 gctcgtcgtc gacgacgccc acggcttcgg ggtgttgggc gagcagggcc gcggcacgct
     1141 gtcgcacttc ggcttgcgca gcgagcgctt catctgcatg ggcacgctcg gcaaggccgc
     1201 cggcgtcgcc ggcgccttcg tcgcggccga tccgaccatc atcgactggc tggtccaggc
     1261 ggcacgaccc tacatctaca cgaccgccgc gccgcccgcc atggcgcatg cgctgcgtac
     1321 gagcctgcag ttgatccgcg gcgacgaggg ggcgcaacgc cgcgtccggc tcgccacgct
     1381 gatcacgctg ctgcgtcgac ggctcggcgc gctcatcgcc gccacgccgc agcggggctg
     1441 gacgctggcc gagtcggcca ccgcgatcca gcccctgatc gtgggcgaca acgaggccgc
     1501 gctggcgctg tcggcggcgc tcgaatcgcg cgggctgtgg gtgccggcca tccgccctcc
     1561 gaccgtcccg gtcggctcgg cgcggctgcg catcacgctg agcgcgtcgc acaccgagga
     1621 cgatgtgctg cggctgggcg acgcgctcga acacgccgcg aaggggttgg cgtgaacgcg
     1681 cgcaccggct tgcgaggctg cttcgtgaca ggcacggaca cggaggtcgg caagacgcgc
     1741 atcagcgcgg cgctgctgca ctgggtggcc agcaggggac gggtcgccgt cggcttcaag
     1801 ccggtcgccg ccggcacgac gctgatcggc gggatgcggg tcaacgagga cgtgcgcctg
     1861 ctgcgcgacg cgagctcggc cggcctcacg gatgccgagg tcggcccgtt ccagttcgac
     1921 gcggcctgtg ccccgcacgt cgccgccgcg ctcgaaggtc gcgcgatcga ccgcgcggcg
     1981 atcctggcgg cgggccgcgc gctgcgggcg cgcggcgact ttctggtggt cgagggggtg
     2041 ggcggtttct gcgtgccgct cggccccgac tgggacagct ccgacatcgc ctgcgggctc
     2101 gcgctgccgg tcattctggt cgtcggcctg cggctcggct gcctgaacca cgcgctgctg
     2161 acggccgagg cgatcaaggc gcgccacctg ccgctggcgg gctggatcgc gaacaccatc
     2221 gatccggcca tggcgtacct ggatcacagc gtgtccacgc tgcggcatga attcgacgag
     2281 cgccggcagc tgccttgtct gggtgtcgtt ccgtggctcg acgaccccca cccggaggcg
     2341 gttgccgcgc atctggatga cgcgggcttg acgctggccc tcggcctgcc cgaacgcgtc
     2401 gcgcccgtcg tgaccctcga cccgccatga cctcgaccgc cgccgcgtct tcgttccggg
     2461 ttgctgcgcc gcggcccgcg ccatggggac gtccgatcgt cgacgccgcg ttcctggccg
     2521 acccctatcc gacctacgct gcgctgcgcg aggccgggcc gattcactgg agcgaggagt
     2581 tcttcggggg tgcgtggctg ctgacccgcc atgccgatgt cgaagccgtg ctgcgcgatg
     2641 ctgcgcgctt ttcggcgcag cgcaccggcg gctgggtgat gcgaagcgat gtcgcgcgcc
     2701 gcgagctgcg gggcttccag cagctgttcg cgcgcgcgat gctgttcgtc gatgccccgg
     2761 atcacgcacg catccgcggc gtgctgaatg cggggttccg cagcgacgtg ctgcagcggt
     2821 tcgcgccggc cgtcgagcgg cgcgtcgatg ccttgctgga tgcggtgaat gcaacgggcg
     2881 ccgacgcagg cttcgacttc atgcaggcct tggcacggcc gctgccggcc gacgtgatgg
     2941 cgatgctgat ggacatcccg gcggccgacc gcggtgactt catcgcctgg tccgacgacc
     3001 tggcatcgtt catcggcgca cccctgccgt cgcacgatcg ggcgcgccgt gcgcaggcca
     3061 gcctgctggc gatgagcggc tacttcgagc gactcgtggc gcagcgccgc gatgcgccgg
     3121 gcgacgacct ggtgagtcgg ctgctgcgcg ccgagacggc cggcgacatc cgatcggggg
     3181 cggagctgct ggcccagtgc gcgatgctgc tgttcgccgg tcacgagacc acgcgcaacc
     3241 tgctgggcaa cggtctgcag gcgctgcttg cgcatccgca ccagtggcag cgcctgcagc
     3301 gcgagcccgg cctgctgccg ggcgcggtgc gcgaactgct gcgcttcgag agcccggtgc
     3361 agtacaccgg ccggcgcgtc gcgaccgacc tggtgctgca cgggcagcag ctgcgccgcg
     3421 gcgatctggt cgtcgcgctg atcggcgcgg ccaac
//
LOCUS       DATMJB010000068         4490 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_641548, whole genome shotgun sequence.
ACCESSION   DATMJB010000068 DATMJB010000000
VERSION     DATMJB010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641548"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VNU48_01605"
     CDS             <1..620
                     /locus_tag="VNU48_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWI10004.1"
                     /translation="KDLVKWNALENPNIIEVGQVLRVVPPGTDPNAASSKPVTTARVE
                     TRPLDAKAAAAASAASGVAPAPSAGATPLAPMPAAREADDDINWAWPAAGPVIANFDD
                     GKVKGVVIGGKPGDPVLAAADGRVVYAGSGLRGYGNLVILKHNNTYLTAYAHNQTLLV
                     KEDQTVRRGQKIAEMGSTDADAVKLHFEIRKQGKPIDPAKLLPPR"
     gene            647..1732
                     /gene="rpoS"
                     /locus_tag="VNU48_01610"
     CDS             647..1732
                     /gene="rpoS"
                     /locus_tag="VNU48_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HWI10005.1"
                     /translation="MVRKRASLDGHVPDPPVGAQRPQALVASAQVNGDARGVEPPPEA
                     GVEIGVALVAPDDDADSALVAERAELAAGESEIGNTLQTYLREIRRAPLLTPQEEFDT
                     ATLARAGDFAARQVMIEHNLRLVVSIAKNYLGRGLPMTDLIEEGNLGLMHSIGKFEPE
                     RGFRFSTYASWWVRQSIERAIMHQARLVRLPVHIVRELNQVLKARRALEALSASAGHS
                     DRPVRVEEVAQALGRPAKEVDALLKFAEQPTSLDAPMERHAGEGAGDSVLDGVADDGA
                     TDPMSLTLSNEVEVLLHHGLGELNEREREVVAGRYGLGDRDPETLEVLAERLGLTRER
                     IRQIQQEALLKLRRRMARQGVDRDSIF"
     gene            1759..2691
                     /locus_tag="VNU48_01615"
     CDS             1759..2691
                     /locus_tag="VNU48_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI10006.1"
                     /translation="MAALLDNIRRANRPPFYRMRPVDARRAYEVVSEILELPRAPLVR
                     VEDLSIPADDGTPLRARVYANARETLPVLLYLHGGGFTIGNLESHDSLCRQLALRSGA
                     AVVALDYRLAPEQRFPVAVDDAWAAMRWLAAHAATLGLDGARLAVGGDSAGGTLAAVC
                     AIHARDIGLALRLQVLITPGTTAYADTPSHAQFANGYLLEAEAIAWFFDQYIAHAQRR
                     DWRFAPLEAEALDALAPACVVLAECDPLVDEGLAYADRLRAAGVPVELELARGVTHDF
                     IKLGRVLPEAGAAQALIGAALRAAFDDRPEGPPA"
     gene            2688..3545
                     /locus_tag="VNU48_01620"
     CDS             2688..3545
                     /locus_tag="VNU48_01620"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HWI10007.1"
                     /translation="MKPSDFRFHDRLRVRWAEIDAQKIVFNGHYLMYFDTAVAGYWRA
                     LAMPYAQTLERLGGDLFVRKASLDYTGSARYDDLLDIGLRCARVGTSSILFSGAVFRQ
                     GELLVSCELVYVFADPQTQTSKPVPDALRAILEAFEAGETMVDVRVDNWQALELHARA
                     IRTEVFIAEQKIPLEMEWDDADASAVHAVALNRFGQPLATGRLLHDAPGVARIGRMAA
                     IQALRGGGVGRAVLDALLHTAAARGDREVRLHAQTSAQGFYARAGFVPVGEAFEEAGI
                     AHIEMTRPL"
     gene            complement(3565..4440)
                     /locus_tag="VNU48_01625"
     CDS             complement(3565..4440)
                     /locus_tag="VNU48_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HWI10008.1"
                     /translation="MCLVALAIDQSRRFPLVVASNRDEYFKRPSAGLAWWAPRPGDPA
                     ILSGRDLESGGTWMGLTAQGRLALLTNVRGVLPLEAGAPSRGRVVTEWLTGREDTGDF
                     WMRTALSGHNGFNLIAADFRRGECFWASNTEAHPQRLDRGVYGLSNGGLDAPWPKVDA
                     LKTRLQAALEAARSVDALATQLFAALADRRPAPDDALPHTGVPIDVERQLSAAFIGTP
                     DQRYGTRCSTLVITEKTARQPITHVYERTFDAAGAAVSLRRVLLRNWPPRYVEDDAHL
                     PVAQAVVSEHELATA"
BASE COUNT          660 a         1573 c         1641 g          616 t
ORIGIN      
        1 ggaaagacct ggtcaagtgg aacgcgctcg agaacccgaa catcatcgag gtcggccagg
       61 tgctgcgcgt cgtgccgccg ggcaccgacc cgaacgccgc atcgagcaag ccggtcacga
      121 ccgcgcgcgt cgagacgcgc ccgctggacg ccaaggcggc cgccgccgca tcggccgcga
      181 gcggtgtcgc gcctgcgccc tcggccggcg cgacaccgct cgcgccgatg ccggcggcgc
      241 gcgaggccga tgacgacatc aactgggcct ggcccgccgc cggcccggtg atcgcgaact
      301 tcgacgacgg caaggtcaag ggcgtggtga tcggcggcaa gcccggcgac ccggtgctcg
      361 cggcagccga cgggcgcgtg gtctatgccg gctcgggcct gcgcggctac ggcaacctgg
      421 tgatcctgaa gcacaacaac acctacctga cggcctacgc ccacaaccag acgctgctgg
      481 tcaaagaaga ccagacggtg cggcgcggtc agaagatcgc cgagatgggc tccaccgatg
      541 ccgacgccgt gaagctgcac ttcgagattc gcaagcaagg caagccgatc gacccggcga
      601 agctgttgcc gccgcgctga cgcgctgcaa ccccggggat gagagcatgg tcagaaagcg
      661 cgcgtcgctc gatggccatg tgcctgatcc accggtcggc gcgcagcgcc cgcaggcgct
      721 cgtcgcgagc gcgcaggtca acggcgatgc gcgcggtgtc gagccgcccc ccgaggcggg
      781 cgtggagatc ggcgtcgcct tggtggcgcc cgacgacgac gcggacagcg cactcgtggc
      841 cgagcgcgcc gaactcgcgg cgggcgaatc ggagatcggc aacacgctgc agacctacct
      901 gcgcgagatc cgccgcgcgc cgctgctgac accgcaggaa gagttcgaca ccgccacgct
      961 cgcgcgggcc ggcgacttcg cggcgcgcca ggtgatgatc gagcacaacc tgcgcctggt
     1021 cgtcagcatc gcgaagaact acctcggccg cggcctgccg atgaccgacc tgatcgaaga
     1081 gggcaacctc gggctgatgc actcgatcgg caagttcgag ccggagcgcg gttttcgttt
     1141 ctcgacctac gcgtcgtggt gggtgcgcca gagcatcgag cgcgcgatca tgcaccaggc
     1201 ccggctggtg cgactgccgg tgcacatcgt gcgcgagctg aaccaggtgc tcaaggcgcg
     1261 gcgcgcgctc gaggccctgt cggccagtgc cggccacagc gaccggccgg tgcgtgtcga
     1321 ggaggtcgcg caggcgctcg gccgcccggc gaaggaagtc gacgcgttgc tcaagttcgc
     1381 cgagcagccg acctcgctgg acgcgccgat ggaacgccac gccggcgaag gcgccggtga
     1441 ctcggtgctc gacggcgtcg ccgacgatgg tgcgaccgat ccgatgagcc tgacgctgtc
     1501 caacgaagtc gaggtgctgt tgcaccacgg gctcggcgag ctcaacgaac gtgagcgcga
     1561 ggtcgtggcc gggcgctatg gcctgggcga ccgcgacccc gagacgctgg aggtgctggc
     1621 cgagcgtctc ggcctgacgc gcgagcgcat ccgccagatc cagcaggagg cgttgctgaa
     1681 gctgcgccgt cgcatggcgc gtcagggcgt cgaccgcgac tcgatcttct gatgcgcgcg
     1741 ccgccgctga cggcccgcat ggcggcactg ctcgacaaca tccggcgtgc gaaccggccg
     1801 ccgttctaca ggatgcgtcc ggtcgatgcg cggcgtgcct atgaagtggt gtccgagatt
     1861 ctcgagctgc cgcgcgcacc gctggttcgc gtcgaagatc tctcgatccc ggccgacgac
     1921 ggcacgccgc tgcgggcacg cgtctacgca aacgcgcgcg agaccttgcc ggtgctgctg
     1981 tacctgcacg gcggcggctt cacgatcggc aacctcgaga gccacgacag cctgtgccgc
     2041 cagctcgcac tgcgcagcgg cgcggcggtg gtcgcgctcg actaccggct cgcgccggag
     2101 cagcgctttc cggtcgcggt cgacgacgcc tgggccgcga tgcgctggct cgccgcgcac
     2161 gcggcgacgc tcggtctcga cggtgcgcgc ctcgcggtcg gcggcgacag cgccggtggc
     2221 acgctcgccg cggtgtgcgc gatccacgcc cgcgacatcg ggctggcgct gcgcctgcag
     2281 gtgctgatca cgcccggtac gaccgcgtac gccgacacgc cgtcgcatgc gcagttcgcg
     2341 aacggctacc tgctcgaggc cgaggcgatc gcgtggttct tcgaccagta catcgcccat
     2401 gcgcagcgtc gcgactggcg cttcgcgccg ctcgaggcgg aggcgctcga cgcgctcgcg
     2461 ccggcctgcg tggtgctggc cgagtgcgac ccgctggtcg acgagggcct ggcctacgcc
     2521 gaccgcctgc gcgcggccgg cgtgccggtc gagctcgagc tcgcacgcgg cgtgacgcac
     2581 gacttcatca agctcgggcg cgtgctgccc gaggccggcg cggcgcaggc gttgatcggc
     2641 gccgcgctgc gtgccgcgtt cgacgaccga cctgaaggcc cgcccgcatg aagccaagcg
     2701 acttccgatt tcacgatcgg ctgcgcgtgc gctgggccga gatcgacgcg cagaagatcg
     2761 tcttcaacgg ccactacctg atgtacttcg acaccgcggt ggccggctac tggcgtgcgc
     2821 tggcgatgcc gtatgcgcag acgctggaac gcctgggcgg cgacctgttc gtgcgcaagg
     2881 ccagcctcga ctacaccggt tcggcgcgct acgacgacct gctcgacatc ggcctgcgct
     2941 gcgcgcgcgt cggcacctcg tcgatcctgt tcagcggcgc ggtgttccgg cagggcgagc
     3001 tgctcgtgtc ctgcgagctg gtctatgtgt tcgccgatcc gcagacgcag acctcgaagc
     3061 cggtgcccga cgcgctgcgc gcgatcctgg aggccttcga ggcgggcgag acgatggtcg
     3121 acgtgcgcgt ggacaactgg caggcgctcg agctgcacgc gcgcgcgatc cgcaccgagg
     3181 tgttcatcgc cgagcagaag atcccgctcg agatggagtg ggacgacgcc gatgcgagcg
     3241 cggtccacgc ggtcgcgctg aaccgcttcg gccagccgct ggccaccggc cggctgctgc
     3301 acgacgcacc cggtgtcgcg cgcatcggcc gcatggcggc gatccaggcg ctgcgtggcg
     3361 gcggcgtcgg ccgggcggtg ctcgacgcgc tgctgcacac ggcagccgcg cgcggcgatc
     3421 gcgaagtgcg cctgcatgcg cagaccagcg cgcagggttt ctatgcgcgc gccggcttcg
     3481 tgccagtggg cgaggcgttc gaggaagccg gcatcgccca catcgagatg acgcggccgc
     3541 tgtagccggc gcgcgagcgc actgtcaggc agtcgcgagc tcgtgctccg agacgacggc
     3601 ctgcgcgacc ggcaggtgtg cgtcgtcctc gacatagcgc ggcggccagt tgcgcagcag
     3661 cacgcgccgc aggctcacgg ccgcgccggc ggcgtcgaac gtgcgctcgt agacgtgcgt
     3721 gatcggctgg cgcgcggtct tctcggtgat gaccagcgtc gagcagcgcg tgccgtagcg
     3781 ctgatccggc gtgccgatga aggcggccga caactggcgc tcgacgtcga tcggcacgcc
     3841 agtgtggggc agggcgtcgt cgggtgcggg tcgacggtcg gcgagcgccg cgaagagttg
     3901 cgtcgcgagc gcatcgaccg accttgccgc ctccagcgcc gcctgcagcc tggtcttgag
     3961 cgcatcgacc ttgggccacg gcgcatcgag cccgccgttc gacaggccgt agacaccgcg
     4021 gtccaggcgc tgcggatgcg cctcggtgtt ggaggcccag aagcattcgc cgcggcggaa
     4081 atcggcggcg atcaggttga agccgttgtg gcccgacagt gcggtgcgca tccagaagtc
     4141 gccggtgtcc tcgcgccccg tgagccactc ggtcacgacg cggccgcgcg acggtgcgcc
     4201 ggcctccagc gggagcacgc cgcgcacgtt ggtcagcaac gcgagccggc cctgcgcggt
     4261 caggcccatc caggtgccgc cggactcgag atcgcggccg ctcaggatcg ccgggtcacc
     4321 gggccggggc gcccaccagg ccaggccggc gctcgggcgc ttgaaatatt cgtcgcggtt
     4381 cgaggccacg accagcggga agcgccggct ctggtcgatc gcgagggcta cgagacacat
     4441 gcggtgaaca cctcggtgtg gcgcggtccg tcgagcagcg gcgccagcac
//
LOCUS       DATMJB010000069         5089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_108362, whole genome shotgun sequence.
ACCESSION   DATMJB010000069 DATMJB010000000
VERSION     DATMJB010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108362"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1133)
                     /gene="dctP"
                     /locus_tag="VNU48_01630"
     CDS             complement(171..1133)
                     /gene="dctP"
                     /locus_tag="VNU48_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HWI10009.1"
                     /translation="MTGRTAAQAGVLKISHQFPGGTLTEGDFRDRLCRRFAADVEKRS
                     NGALKAAVYPGSSLMKTNSQFAALRKGALDLSLVPLSYAGGEVAETNIGLMPALVPSY
                     EVGAAWKTSEVGKMLAKVLDEKGIVVLSWIWQAGGVASRARPLVAPDDAKGQKVRGGS
                     REMDLMLKAAGASVITLPSNEIYAAMQTGAMDAAMTSSTSFISFKLEEIAKHLTTGRN
                     KAYWFMFEPLLMSKEIFAKLARPQQDIVMAVGAEQEQFATEAARADDRNAADVYTKAG
                     AKVYDIDEPTMKKWQAIARETAWKDFAERNESCKALLTAAQKLL"
     gene            1451..1717
                     /locus_tag="VNU48_01635"
     CDS             1451..1717
                     /locus_tag="VNU48_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10010.1"
                     /translation="MSHQHQDAATTHHSGIDHSSSAPPMAIPPGHVGEFVIPGTGRRV
                     WWTGRVAIGLLYQPQRCREPAAQSSIWIQTLMLCKPQRTSPAFG"
     gene            complement(1735..2229)
                     /locus_tag="VNU48_01640"
     CDS             complement(1735..2229)
                     /locus_tag="VNU48_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10011.1"
                     /translation="MIAARTRAGSQVALRYRGPMKTFVVALLSISGLALGRAALASEP
                     SPRDLHAAQCVAALEVNTQDLAQQVKSGKEASRPVLLDRLVAGTAFVGDTYLHGSSDE
                     QQARALASRALEAQKSLSAPELAARQAACAAEGAKLFASSNGLQQAVVKRLAKKRMDK
                     LLST"
     gene            complement(2226..2483)
                     /locus_tag="VNU48_01645"
     CDS             complement(2226..2483)
                     /locus_tag="VNU48_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10012.1"
                     /translation="MKASHVLAALALSLTAAVSAQAQTPDAPAHGDFASRKSAILAHL
                     DQRMAAMSAMRACVANAAARVDLKTCHEQHRAAMKPNRAPT"
     gene            2759..3988
                     /locus_tag="VNU48_01650"
     CDS             2759..3988
                     /locus_tag="VNU48_01650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWI10013.1"
                     /translation="MHIDIASLLVAMTLCMVTMAVALPAVMGQVNEAARRAQAGVMLQ
                     AAGWAMLLISATVDAGSSLDVACSTLAMVGIASGLALLATSFDLWCGRKAHDRATLFI
                     AIVMPLGYALGFSSYAFRVGWANGLLTLQMGLVAVALARKPAVPVGRWRGLLVFSLLA
                     QMLVTAGRGVLGAFFTESYPTFLAPHPMNLAFALLANATTVLSLVGILLAHRDEAARE
                     LERLATVDGLTNVLNRRAWKTLARTELAVSARYGHPVAVLMIDLDRFKHINDTRGHDA
                     GDRALCFVAAALQTAVRPGDLVGRYGGEEFCVLMSHADLDATVAFDARMRAHLAEAAA
                     TELGFTLSYSAGIATRTSESDTLEAMLQRADANLYAAKHQGRARTLDSNGVSVHDTFT
                     RNSGNYAAIAAALRAPS"
     gene            complement(4033..5022)
                     /locus_tag="VNU48_01655"
     CDS             complement(4033..5022)
                     /locus_tag="VNU48_01655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI10014.1"
                     /translation="MHSGLVAPIVSLHRVYGWIFLIDKLGAEAFAEDDERILAIYAAQ
                     AGRIYENGSLYKRIKDTAEQLEIEIEQRKRVAQDLIVANDGLEQRVKQRTAELRDVIE
                     GLESFNRSVSHDLRGPLGGIAGAARLAQEFMAENRNEKAAQFLQIIATQADTTAQLVD
                     ALLALARASDASLSRQRVDMSALTSEVIESLRQAHPESPMPIVVGPLTDIDADRELVR
                     QVLVNLIGNALKFASEVPHPEIHVGMRGAGHASVFFVGDNGVGFDDTQAQRLFKPFHR
                     AHGTRFKGSGVGLSIVKRIVDRHGGEVWAESAPGRGATFYFSFGGVTPSSASV"
BASE COUNT          769 a         1772 c         1688 g          860 t
ORIGIN      
        1 gctggaggtc agcaccaccg aagcggccag cagcgccacc atgcccagcc ccgtcatcgc
       61 acggttgacg aggcgcagcg cacgcgccag cggctgcagc cagcgcggcg tggccgggtc
      121 gatggcgtcg ctgtcggcgc cggcgccgag gggcgcctcc agggccgcgc tcacagcagc
      181 ttctgggcgg ccgtcagcaa tgccttgcaa gactcattgc gctcggcgaa gtccttccag
      241 gcggtctcgc gggcgatcgc ctgccacttc ttcatggtgg gctcgtcgat gtcgtagacc
      301 ttcgcgccgg ccttcgtgta cacatcagcg gcgttgcggt cgtcggcgcg cgcggcctcg
      361 gtggcgaact gctcttgctc ggcgccgacc gccatgacga tgtcctgctg cggcctggcc
      421 agcttggcga agatctcctt cgacatcagc agcggctcga acatgaacca gtaggccttg
      481 ttgcgaccgg tcgtcaggtg cttggcgatc tcttcgagct tgaacgagat gaagctggtc
      541 gatgaggtca tcgcggcgtc catcgcgccg gtctgcatcg ccgcatagat ctcgttcgac
      601 ggcagcgtga tcaccgaggc gccggcggcc ttgagcatca ggtccatctc gcggctgcca
      661 ccgcgcacct tctggccctt cgcatcgtcg ggcgcgacca aggggcgcgc gcgactcgcg
      721 acaccgccgg cctgccagat ccagctcagc acgacgatgc ctttttcgtc gagaaccttc
      781 gcgagcatct tgccgacctc ggaggtcttc cacgcggcgc cgacctcgta gctcggcacc
      841 agcgcgggca tcaggccgat gttggtctcg gccacttcgc cgccggcgta cgacagcggc
      901 accaggctca ggtcgagcgc gcccttgcgc agcgccgcga actgcgagtt ggtcttcatc
      961 agtgacgagc cggggtagac cgcggccttc agcgcgccgt tgctgcgctt ctcgacgtcg
     1021 gcagcgaagc gccggcacag ccggtcgcgg aagtcgcctt cggtcagcgt cccgcccggg
     1081 aactggtgcg agatcttcag gacaccggcc tgcgcggctg tgcgcccggt catcgatgcg
     1141 acaccgaccg ccgccgcggc ccgaatcaat tcacgcctcg tcatcgccat gtcatcgtct
     1201 cctgtcgcca tcgaaaaagc gtcgaactcc ggcaccgcgc tggccctcgg gtccgagacg
     1261 atgatctttg cacgcatgaa ttcgccccaa accggggaac taccctttgc cgcccgggcg
     1321 aaaacgcgac gctcgcggcc gcgccacccc ctaaatcggg tgagctccga gtgttgcctt
     1381 ccccctcact gaggtggtgt ccgatcccgc gatcggatcg atcatggaag caccacttca
     1441 ggagtcgacc atgtctcacc aacatcagga cgccgcaacg acccaccaca gcggcatcga
     1501 tcacagctcg tcggcaccgc cgatggcgat cccgcccggc catgtcggcg agttcgtgat
     1561 cccggggacc ggccggcgcg tctggtggac cgggcgcgtc gcgatcggcc tgctgtatca
     1621 gccccagcgc tgccgggagc ccgcggcgca atcgtcgatc tggatccaga cgctgatgct
     1681 ctgcaagccg caacgcacga gccccgcgtt cggctgaggg cgcgcccagg cggctcaggt
     1741 gctgagcagc ttgtccatgc gcttcttcgc gagacgcttg acaacggcct gctgcaggcc
     1801 gttgctggac gcgaacagtt tcgcgccttc ggcggcgcac gcggcctgac gcgccgccag
     1861 ttccggcgcc gacaggctct tctgcgcctc gagcgcccgg ctggcgaggg cgcgggcctg
     1921 ctgctcgtcg ctgctgccgt gcagataggt atcgcccacg aacgcggtgc ccgccacgag
     1981 gcggtccagc agcacggggc gagacgcctc cttgcctgac ttgacctgtt gcgcgaggtc
     2041 ctgcgtgttg acctcgagcg ccgcgacgca ttgggccgcg tgcagatcgc gcggcgacgg
     2101 ctcggaggcc agtgcagccc ggccgagcgc gaggccggag atcgacagca gtgcgacgac
     2161 gaaagtcttc atggggccac gatagcgaag tgcgacttgg ctgccggcac gcgtgcgtgc
     2221 ggcgatcaag tcggcgcgcg gttcggcttc atcgcagcgc gatgctgttc gtgacaggtc
     2281 ttcaggtcca cccgggccgc ggcgttggcg acacacgcgc gcatcgcgga catcgccgcc
     2341 atgcgctggt cgaggtgcgc gaggatcgcg gacttgcggg acgcgaagtc gccgtgcgct
     2401 ggggcatcgg gcgtctgggc ctgcgccgag acggccgcgg tgagggacag ggccagggcg
     2461 gcgagaacgt gggatgcttt cacgagaggc tccggtgatg gacagtgact cctccacaac
     2521 gcgcctcacc ggcaggcgtg cacaggcttt gcgcctcttc actttccgca agggcgcttc
     2581 acgtctggcg cgctccctct cgccttcgac ggaaggtcga cgccatcgtt ccagaccgtt
     2641 tcaccgtgcc ggccagcgga ctgcggcacg gcctgccgtc tgttcgcggc tctgaggacg
     2701 ccgccagcca cgacaatctc gcaagactca cttcgtcgcc aacgacaccc ggccacccat
     2761 gcacatcgac atcgccagtc tcctcgtcgc catgaccttg tgcatggtca cgatggcggt
     2821 cgcgcttcct gcggtgatgg gccaggtcaa cgaggcggcc cggcgcgcgc aggccggcgt
     2881 catgctgcag gccgccgggt gggccatgtt gctgatatcc gcaacggtgg acgccggctc
     2941 ctcgctggac gtggcctgct ccacgctggc catggtgggc atcgccagcg ggctggcgct
     3001 tctggcgact tcattcgacc tgtggtgcgg caggaaggcg cacgaccggg cgaccctgtt
     3061 catcgccatc gtcatgccgc tgggctacgc gctgggattc tcgagctacg cgttccgcgt
     3121 gggctgggcc aacgggctgc tgacgctgca gatggggctc gtggcggtcg cgctggctcg
     3181 caagccggcc gttccggtcg gtcgctggcg cgggctgctc gtgttcagct tgctggcgca
     3241 gatgctggtg acggccggcc gcggcgttct cggcgccttc ttcaccgaga gctatccgac
     3301 gttcctggcg cctcacccga tgaatctggc attcgcgctg ctcgccaacg cgaccaccgt
     3361 gctgtccctg gtgggcatcc tgctggcaca tcgagacgaa gccgcacgcg agctggagcg
     3421 actggccacc gtcgacggcc tgaccaacgt gctcaatcgc cgggcctgga agacgctggc
     3481 gcgcaccgaa ctcgcggtca gcgcgcgcta cggccatccg gtggccgtgc tgatgatcga
     3541 cctggaccgc ttcaagcaca tcaacgacac ccggggccat gacgcgggcg accgcgcgct
     3601 gtgcttcgtc gccgcggcgc tgcaaacggc cgtgcgcccc ggcgacctgg tcggccgcta
     3661 cggcggcgag gagttctgcg tgctgatgag ccatgcagac ctcgatgcca ccgtggcctt
     3721 cgacgcgcgc atgcgggcgc acctggccga ggccgcagct actgagctcg gcttcacgct
     3781 cagctacagc gccggcatcg ccacgcgcac ctcggagagc gacacgctcg aagccatgct
     3841 ccagcgtgcg gacgcgaacc tctatgccgc caagcatcag ggccgggccc gcacgctcga
     3901 ttcaaacggc gtcagcgttc acgacacctt cacgagaaac tccggcaact acgcggcgat
     3961 cgcggccgcg ctgcgggcac cttcatagcc acggtcttcc ggacggttcg ttgcgcgcgt
     4021 cgacaggtct tgtcagacgc tcgccgaaga aggcgtgact ccgccgaaac tgaagtagaa
     4081 cgtggcgccg cggcccggtg cgctctcggc ccagacttct ccgccatggc gatcgacgat
     4141 gcgtttgaca atcgacaggc cgacgcccga gcccttgaag cgcgtgccgt gcgcgcgatg
     4201 aaacggcttg aacaggcgct gcgcctgcgt gtcgtcgaag ccaacgccgt tgtcgcccac
     4261 gaagaacacc gacgcgtggc ccgcaccgcg catccccaca tggatctcgg gatgcggcac
     4321 ttcggaggcg aacttcaacg cattgccgat caggttcacc agcacctgtc gcaccagttc
     4381 gcggtcggcg tcgatgtcgg tcagcggacc gacgacgatc ggcatcggtg attcggggtg
     4441 cgcctgccgc aaggactcga tcacttcgga ggtgagcgcg ctcatgtcga cgcgctgtct
     4501 cgacaggctc gcgtcgctcg ctcgagcgag ggccagcaag gcatccacca gctgcgcggt
     4561 ggtgtccgct tgcgtggcga tgatctggag gaactgggcc gccttctcgt tgcggttttc
     4621 cgccatgaac tcctgcgcaa gccgtgccgc accggcgatg ccgccgagcg gcccgcgcag
     4681 atcgtgcgac acgctgcggt tgaagctctc cagcccctcg atcacgtcgc gcaactcggc
     4741 cgtgcgctgc ttgacgcgtt gctcgaggcc gtcgttggcg acgatcaggt cctgcgcgac
     4801 gcgcttgcgt tgttcgatct cgatctccag ctgttccgcg gtgtccttga tccgcttgta
     4861 caggctgccg ttctcgtaga tgcgtccggc ctgggcggca tagatggcca ggatgcgctc
     4921 gtcgtcttcg gcgaacgcct ccgcgcccag cttgtcgatc aggaagatcc acccgtagac
     4981 acggtgcaga gaaacgatcg gtgccacgag gccgctgtgc acggacggaa aatccttcgg
     5041 aaggtccgcg ccgatcgggt caccccctga attcacgaag cgtcgggcg
//
LOCUS       DATMJB010000070         2894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_641133, whole genome shotgun sequence.
ACCESSION   DATMJB010000070 DATMJB010000000
VERSION     DATMJB010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641133"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VNU48_01660"
     CDS             complement(<1..1078)
                     /locus_tag="VNU48_01660"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWI10015.1"
                     /translation="MRDPLMSVEPTPPNPPVAGLAPAEHDALPAGTRFGEFEILRVLG
                     AGGFGIVYLAQDHSLERAVALKEYMPAMLAARGAGATITIRARAFAETYANGLRSFVN
                     EARLLARFDHPSLVKVYRVWEDNGTAYMVMPYLPGRTLRDLRREMIDAPDEAWLRGLL
                     DPLLDALDALHREGVFHRDIAPDNILLPPDGAPILLDFGAARRVIGDAAQSLTAIVKP
                     SYAPIEQYAEMAGLRQGPWTDLYALGAVLHFLLFGAPPAPATARALQPDAEPIGQRRV
                     AGVSPPFMAAVGWMLGVRPSARPQSVAALRAALAGELPIPVPLHDAVTLPADLARAAI
                     DTAARPAAGRWAVDRTQQTTAPAIA"
     gene            1211..2545
                     /gene="argG"
                     /locus_tag="VNU48_01665"
     CDS             1211..2545
                     /gene="argG"
                     /locus_tag="VNU48_01665"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWI10016.1"
                     /translation="MANILQQLPVGQKVGIAFSGGLDTSAALHWMRNKGAIPYAYTAN
                     LGQPDEADYDEIPRKAIEYGAQAARLIDCRAQLVAEGIAALQSGAFHISTGGITYFNT
                     TPLGRAVTGTMLVAAMKDDDVNIWGDGSTYKGNDIERFYRYGLLANPGLKIYKPWLDQ
                     LFIDELGGRAEMSAFMSAAGFKYKMSAEKAYSTDSNMLGATHEAKDLEHLSSGMKIVQ
                     PIMGVAFWRDDVEVKREEVTVRFEEGQPVALNGMRFASAVELLLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGLALLFVAYERLVTGIHNEDTIEQYRDNGRRLGRLLYQG
                     RWFDPQAIMLRETAQRWVASAVTGEVTLELRRGNDYSLLNTESPNLTYKPERLSMEKV
                     EGAFSPQDRIGQLTMRNLDIADTREKLGIYAGTGLLAAGGAADLLRLGKGGG"
     gene            2580..>2894
                     /locus_tag="VNU48_01670"
     CDS             2580..>2894
                     /locus_tag="VNU48_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10017.1"
                     /translation="MRRLPPSLKQLAARGLLALAFLFAQQTAALHWLSHAIEATQAKA
                     DKAGAPVDHCDECLALGALGAAATSGAAPLPLALAQHAQRSALPETPAPAALRLAFRS
                     RAP"
BASE COUNT          444 a         1024 c         1041 g          385 t
ORIGIN      
        1 gcgcgatcgc cggcgccgtg gtctgctgcg tgcggtcgac ggcccagcgc ccggcggcgg
       61 gccgggccgc cgtgtcgatc gccgcacgcg cgagatcggc gggcagcgtg accgcgtcat
      121 gcagcggcac cgggatcggc agctcgcccg cgagcgcagc gcgcaacgcc gcgacgcttt
      181 gcggccgcgc ggacggtcgc acgccgagca tccagccgac cgcagccatg aatggcggcg
      241 agacgccggc cacgcggcgc tgcccgatcg gctcggcgtc gggctgcagc gcgcgtgcgg
      301 tcgccggcgc cggcggcgcg ccgaacagca ggaagtgcag caccgcgccg agcgcgtaca
      361 ggtcggtcca cggaccctgg cgcagcccgg ccatctcggc gtactgctcg atcggtgcgt
      421 agctgggttt cactatcgcg gtcagcgact gcgcggcgtc gccgatgacg cggcgcgccg
      481 cgccgaagtc gagcaggatc ggcgcaccgt cgggcggcag caggatgttg tcgggcgcga
      541 tgtcgcggtg gaacacgcct tcgcggtgca gcgcgtcgag cgcgtcgagc agcgggtcga
      601 gcaggccgcg cagccaggcc tcgtcgggcg cgtcgatcat ctcgcggcgc aggtcgcgca
      661 gggtccggcc gggcaggtag ggcatcacca tgtaggcggt gccgttgtcc tcccagacgc
      721 ggtacacctt gaccagcgac gggtggtcga agcgcgccag cagccgcgcc tcgttgacga
      781 acgagcgcag cccgttcgcg taggtctcgg cgaaggcgcg cgcgcggatc gtgatcgtcg
      841 cgcccgcgcc gcgcgccgcc agcatcgccg gcatgtactc cttcagcgcg accgcacgtt
      901 cgagcgagtg gtcctgcgcg aggtagacga tgccgaagcc gccggcgccg agcacgcgca
      961 ggatctcgaa ctcgccgaag cgcgtgccgg cgggcaaggc gtcgtgctcg gccggtgcga
     1021 gcccggcgac gggcggattc ggtggggtcg gttcgacgct catgagcggg tcgcgcatcg
     1081 ctgcatcgac caccgagctt aacgcagggc cggcggcgcg cgccggatcc gtactgcggc
     1141 ttcgggccac tttcgggctt gcacctaaaa ttgcaggttc tcctggccga acctcacccc
     1201 ccgtcttccc atggcaaaca ttcttcagca gctgcccgtc gggcaaaagg tcggcatcgc
     1261 attctccggc gggctggaca ccagcgcggc gctgcactgg atgcgcaaca agggcgcgat
     1321 cccctacgcc tacaccgcga acctgggcca gcccgacgag gccgactacg acgagatccc
     1381 gcgcaaggcg atcgaatacg gcgcgcaggc cgcgcgcctg atcgactgcc gcgcgcagct
     1441 ggtcgccgag ggcatcgcgg cgctgcaaag cggggcgttc cacatctcga ccggcggcat
     1501 cacgtacttc aacaccacgc cgctggggcg cgccgtcacc ggcacgatgc tggtcgcggc
     1561 gatgaaggac gacgacgtca acatctgggg cgacggcagc acctacaagg gcaacgacat
     1621 cgagcgcttc taccgctacg gcctgctcgc gaatccgggg ctgaagatct acaagccctg
     1681 gctggatcag ctcttcatcg acgagctcgg cgggcgcgcc gagatgtcgg cgttcatgag
     1741 cgcggccggc ttcaagtaca agatgtcggc cgagaaggcc tactcgaccg actcgaacat
     1801 gctgggcgcg acgcacgagg cgaaggatct cgagcacctg tccagcggca tgaagatcgt
     1861 gcagccgatc atgggcgtcg cgttctggcg cgacgacgtc gaggtcaagc gcgaggaagt
     1921 cacggtgcgc ttcgaggagg gccagccggt cgcgctcaac ggcatgcggt ttgccagcgc
     1981 ggtcgagttg ctgctggagg cgaaccgcat cggcgggcgc cacggcctgg gcatgagcga
     2041 ccagatcgag aaccgcatca tcgaggcgaa gagccgcggc atctacgagg cgccgggcct
     2101 ggcgctgctg ttcgtcgcct acgagcggct cgtcaccggc atccacaacg aggacacgat
     2161 cgagcagtac cgcgacaacg gccggcgcct gggccgcctg ctctaccagg gtcgctggtt
     2221 cgacccgcag gcgatcatgc tgcgcgagac cgcgcagcgc tgggtcgcca gcgcggtgac
     2281 cggcgaggtc acgctcgagc tgcgccgcgg caacgactac tcgctgctga acaccgagtc
     2341 gccgaacctg acctacaagc ccgagcggct gagcatggag aaggtcgagg gcgcgttctc
     2401 gccgcaggac cgcatcggcc agctgacgat gcgcaacctc gacatcgccg acacgcgcga
     2461 gaagctcggc atctacgccg gcaccgggct gctcgcggcc ggcggcgcgg ccgacctgct
     2521 gcgcctgggc aagggcggcg gctgacgcgg ttcgcgccgc agtcatagac tctcgcgtca
     2581 tgcgtcgcct cccgccctcg ctgaagcagc tcgccgcgcg cggcctgctc gcgctggcat
     2641 tcctgttcgc gcagcagacc gcggcgctgc attggctgtc gcatgcgatc gaggcgacgc
     2701 aggcgaaggc cgacaaggcc ggtgcgccgg tcgaccattg cgacgagtgc ctggcactcg
     2761 gcgcgctcgg cgccgccgcg accagcggcg ccgcgccgct gccgctcgcg ctggcgcagc
     2821 atgcgcagcg ctccgccctt cccgagacgc ccgcgccggc cgcgctgcgg ctggcgttcc
     2881 gttcgcgcgc ccca
//
LOCUS       DATMJB010000071         5413 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_759903, whole genome shotgun sequence.
ACCESSION   DATMJB010000071 DATMJB010000000
VERSION     DATMJB010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759903"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1091)
                     /gene="murG"
                     /locus_tag="VNU48_01675"
     CDS             complement(18..1091)
                     /gene="murG"
                     /locus_tag="VNU48_01675"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HWI10018.1"
                     /translation="MKHLMVMAAGTGGHVIPGLAVAREMQSRGWSVSWLGTPSGMENK
                     LVPPSGIPIDTIGFSGLRGKGLLHTMTGGLRLLAAFWSCLRIIRRRRTTAVLGMGGYV
                     CFPGGLMASLLGRPLVLVNADASLLMSNRALLPVADAVAFGFDGAAAHKTKHAVVTGN
                     PVRAEIEALPAPAQRFAGRAGALRVLVVGGSLGARALNDCVPQAVALLAAGQRPQVTH
                     QTGEANLASVRSAYAAAQVDAQVLPFIDDMAAQLADCDVILCRAGAVTVSELCAAGVA
                     AVLVPLIVSTTSHQRDNAQWLAGQGAGIHLPQSELSPRSLAELLTGLTRDALLAMATK
                     ARALAKPKAAARVADQIERLALA"
     gene            complement(1240..2481)
                     /gene="ftsW"
                     /locus_tag="VNU48_01680"
     CDS             complement(1240..2481)
                     /gene="ftsW"
                     /locus_tag="VNU48_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HWI10019.1"
                     /translation="MPKRGDKPAEGKRVRAPKPIPVRDWIDVGKSGQPARLLGFDQAL
                     VCVVVALLALGIVMVYSATVALPDSPKFARYAPTYFLSRQAMFVAMSLVAALVTVQIP
                     VAFWEKASPWIFVGSLLLLVVVLIPGIGKGVNGAHRWIPLGLLNFQPSELAKVAIAMY
                     AASYMMRKMDIKERFVRAVAPMAVALAVIGLLLLAEPDMGAFMVIAVIAMGILFLGGV
                     NGRMFFLSIAVLVGAFVLMIISSPWRAERILAFLKPWDPQYAMGKGYQLTHSLIALGR
                     GEIFGQGLGGSVEKLHYLPEAHTDFLLAVIGEELGFVGVAFVIFAFFWIARRLFHIGR
                     QAIALDRVFAGLYAQGIGIWMGGQAFINMGVNLGVLPTKGLTLPLMSYGGSAIVMNIV
                     ALAIVLRIDIENRQLMRGGRA"
     gene            complement(2535..4313)
                     /gene="murD"
                     /locus_tag="VNU48_01685"
     CDS             complement(2535..4313)
                     /gene="murD"
                     /locus_tag="VNU48_01685"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWI10020.1"
                     /translation="MNDLQDTTVLVLGLGESGLAMARWCARGGADVRVWDSREQPANA
                     AALAEQVPAAQWLRGPLTDAAFAGVARVLKSPGLSPQDERIAVPLSRAADRGVPVQGE
                     LDLFARALADLKIERGYAPKVLAITGTNGKTTTTAMTAQLIARAGRRVAVAGNIGPTM
                     LQTLADALAREPAPTDAAANEPDLLDAASDPEDLADLRALDGAPANDGDAAIDSTPAP
                     LSDDDAAPVPLVLAPVRPAAPVFEVLPEVWVLELSSFQLDEVRGFEPSAAALLNISED
                     HLDWHGSMAHYIAAKARVFGRHAVMVVNRDDPLVEALVPAPEIIKGAKGRKPTVVARQ
                     VVRFGLSAPRVPGDFGLVDENGMTWLVRARELDETLKRSKGGKGKGGSEERAEPEELL
                     IQRLMPADALRVRGRHNAANALAALALATAIDCPLAPMLHGLREYAGEPHRVQYLATL
                     DGVDAFDDSKGTNVGATVAALNGLGADKAPARLVVILGGDGKGQDFAPLVDPVRRHVR
                     AVATIGRDAERIERALAPANVPMQRHDTLQAATRWCFERAQPGDAVLLSPACASLDMF
                     RNYGHRAEVFVAEVHAIVADHGGLAA"
     gene            complement(4316..>5413)
                     /gene="mraY"
                     /locus_tag="VNU48_01690"
     CDS             complement(4316..>5413)
                     /gene="mraY"
                     /locus_tag="VNU48_01690"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWI10021.1"
                     /translation="RAVMASLTALLIGLAFGPWVINRLTELKIGQPIREYGVKAHLAK
                     AGTPTMGGVLVLFSIAFATLLWFDLSNRFVWIVLWVTLGFGAIGWVDDWRKVVRKNPE
                     GMPSGEKYFWQSVIGLVAALYLAFSVSETSNLRVLELFIRWVQSGFANELPPKADLMV
                     PFFKTISYPLGVFGFIALTYFVIVGASNAVNLTDGLDGLAIMPVVMVGSALGIFAYIT
                     GSSVYSKYLLFPYIPGAGELLIFCAAMAGAGLAFLWFNTHPAQVFMGDVGALSLGGAL
                     GTIAVITRQEIVLGIMGGVFVAEALSVMVQVMWFKYTKKKYGAGRRILKMAPLHHHFE
                     KSGWKETQVVVRFWIVTMLLCLIGLASLKLR"
BASE COUNT          832 a         1961 c         1868 g          752 t
ORIGIN      
        1 gtgcttgacg gcgtgcttca tgcgagcgcc agcctttcga tttgatccgc gacacgcgcc
       61 gcggccttcg gcttcgccag cgcgcgcgcc ttggtcgcca tggccagcag cgcgtcgcgc
      121 gtcaacccgg tcagcagctc cgcgagcgag cgcggcgaca gctcgctctg cggcaggtgg
      181 atgcccgcgc cctgcccggc cagccattgc gcgttgtcgc gctggtgcga ggtggtgctg
      241 acgatcagcg gcaccagcac cgcggcgacg ccggccgcac acagctcgct gaccgtcacc
      301 gcgccggcgc ggcagaggat gacgtcgcag tcggcgagct gcgcggccat gtcgtcgatg
      361 aagggcagca cctgcgcgtc gacctgcgcc gcggcgtagg cggagcgcac gctcgcgaga
      421 ttcgcctcgc cggtctggtg cgtgacctgc gggcgctgcc cggccgcgag cagcgcgacc
      481 gcctgcggca cgcagtcgtt caacgcgcgc gcgccgaggc tgccgccgac gacgagcacg
      541 cgcagcgcgc cggcgcggcc ggcgaagcgc tgcgccggcg ccggcagcgc ctcgatctcg
      601 gcgcgcaccg ggttgccggt cacgaccgcg tgtttggtct tgtgcgcggc cgcgccgtcg
      661 aagccgaacg cgacggcgtc ggccaccggc agcagcgccc ggttgctcat cagcagagac
      721 gcatcggcgt tgaccagcac cagcggtcgg ccgagcagcg aggccatcag gccgccgggg
      781 aagcagacgt agccgcccat gccgagcacc gcggtcgtgc ggcggcggcg gatgatccgc
      841 aggcaactcc agaaggcggc cagcagccgc aggcccccgg tcatcgtgtg cagcaagccc
      901 ttgccgcgca ggcccgagaa gccgatcgtg tcgatcggga tgccgctcgg cggcacgagc
      961 ttgttttcca tgcccgaagg cgtgccgagc cagctgaccg accagccgcg cgactgcatc
     1021 tcgcgcgcca ctgccaggcc cgggatcacg tgcccgccgg tgcccgcggc catcaccatc
     1081 aggtgcttca cgcacgacct ccgcgcatga gctgtctgtt ttggctgcgg cccgcgcact
     1141 gcgtgcgctt cacatcgctg cgcgatgtga gcctgcgccg gaatcgccgg ccggcgctac
     1201 gcgccgacgc ctcgttcccg ctgcgcgcga acaaggcgtt catgcgcggc ccccgcgcat
     1261 gagctggcga ttctcaatat cgatgcgcag cacgatcgcg agcgcgacga tgttcatcac
     1321 gatcgccgaa ccgccgtagc tcatcagcgg cagcgtcagc cccttggtcg gcagcacgcc
     1381 caggttcacg cccatgttga tgaaggcctg gccgcccatc cagatgccga tgccctgcgc
     1441 gtacaggccg gcgaacacgc ggtcgagcgc gatcgcctgg cggccgatgt ggaacaggcg
     1501 ccgcgcgatc cagaagaagg cgaagatcac gaaggcgacg ccgacgaagc cgagctcttc
     1561 gccgatcacc gcgagcagga agtcggtgtg cgcctcgggc aggtagtgca gcttctcgac
     1621 gctgccgccc agcccttggc cgaagatctc gccgcggccg agcgcgatca gcgagtgcgt
     1681 cagctggtag cccttgccca tcgcgtactg cgggtcccag ggcttcagga acgcgaggat
     1741 gcgctcggcc cgccacgggc tgctgatgat catcagcacg aacgcgccga ccagcaccgc
     1801 gatgctgagg aagaacatgc gcccgttcac gccgcccagg aacaggatgc ccatcgcgat
     1861 caccgcgatc accatgaacg cgcccatgtc gggctcggcc agcagcaaga ggccgatcac
     1921 cgcgagcgcc accgccatcg gtgcgaccgc gcgcacgaag cgctccttga tgtccatctt
     1981 gcgcatcatg tagctcgccg cgtacatcgc gatcgccacc ttcgcgagct cggacggctg
     2041 gaagttcagc aaccccagag ggatccagcg gtgcgcgccg ttcacgccct tgccgatgcc
     2101 cgggatcagc accaccacca gcagcagcag cgagccgacg aagatccacg gcgaggcctt
     2161 ctcccagaag gccacgggga tctgcaccgt gaccagcgcc gcgaccagcg acatcgcgac
     2221 gaacatcgcc tggcgcgaca ggaagtaggt cggcgcgtag cgcgcgaact tcgggctgtc
     2281 gggcagcgcg acggtggcgc tgtagaccat cacgatgccc agcgcgagca gcgcgaccac
     2341 gacgcacacc agcgcctggt cgaagccgag caggcgcgcc ggctggccgc tcttgccgac
     2401 gtcgatccag tcgcgcaccg gaatcggctt cggcgcgcgc acgcgcttgc cctcggccgg
     2461 cttgtcgccg cgctttggca tcaagccggc gacgcgctcg cgcagcgcat cgaaccaggt
     2521 cagcgtcgcg gtgctcatgc cgccaaccct ccgtggtcag cgacgatcgc gtgcacctcg
     2581 gcgacgaaca cctcggcgcg gtggccgtag ttgcggaaca tgtccaggct cgcgcaggcc
     2641 gggctcagca gcacggcgtc gccgggctgc gcgcgctcga agcaccagcg cgtcgcggcc
     2701 tgcagtgtgt cgtggcgctg catcggcaca ttcgcggggg cgagcgcgcg ttcgatgcgt
     2761 tcggcgtcgc ggccgatggt cgcgaccgcg cgcacgtggc ggcgcaccgg gtcgacgagc
     2821 ggtgcgaagt cctgcccctt gccgtcgccg ccgaggatca cgacgagccg cgccggcgcc
     2881 ttgtcggcac ccaggccgtt cagcgccgcg acggtcgcgc cgacgttcgt gcccttgctg
     2941 tcgtcgaacg catcgacacc gtccagcgtc gcgaggtact gcacgcggtg cggctcgccg
     3001 gcgtactcgc gcaggccgtg cagcatcggc gcgagcgggc agtcgatcgc ggtcgcgagc
     3061 gcgagcgcgg ccagcgcgtt cgccgcgttg tggcgcccgc gcacgcgcag cgcgtcggcg
     3121 ggcatcaggc gctggatcag cagctcttcg ggttcggcgc gctcttcgct gccgcccttg
     3181 cccttgccgc ccttgctgcg cttgagcgtc tcgtcgagtt cgcgcgcgcg caccagccag
     3241 gtcatgccgt tctcgtcgac caggccgaag tcgccgggca cgcgcggcgc gctcaggccg
     3301 aagcgcacga cctggcgcgc gaccacggtc ggcttgcgcc ccttcgcgcc cttgatgatt
     3361 tccggcgccg gcaccagcgc ctcgaccagc gggtcgtcgc ggttgaccac catcaccgcg
     3421 tgccggccaa acacgcgcgc cttcgcggcg atgtagtgcg ccatcgagcc gtgccagtcg
     3481 aggtggtctt cgctgatgtt cagcagcgcc gcggcgctgg gctcgaagcc gcgcacctcg
     3541 tcgagctgga agctcgacag ctcgagcacc cagacctcgg gcagcacctc gaacacgggc
     3601 gcggccggcc gcaccggcgc gagaacgagc ggcacgggcg cggcgtcgtc gtcggacagc
     3661 ggcgcgggtg tcgagtcgat cgccgcgtcg ccgtcgttcg ccggcgcgcc atcgagcgcg
     3721 cgcagatcgg ccaggtcctc ggggtctgac gcggcgtcga gcaggtccgg ctcgttcgcg
     3781 gcggcgtccg ttggtgccgg ctcgcgggcc agcgcgtcgg ccagcgtctg cagcatcgtc
     3841 gggccgatgt tgccggccac cgcgacgcgc cggccggcgc gcgcgatcag ctgcgcggtc
     3901 atcgcggtcg tcgtcgtctt gccgttcgtg ccggtgatcg ccagcacctt gggcgcgtag
     3961 ccgcgctcga tcttcaggtc ggccagtgcg cgggcgaaca ggtcgagttc gccctgcacc
     4021 ggcacgccgc ggtcggcggc gcggctcagc ggcacggcga tgcgctcgtc ctgcggcgac
     4081 aggcccgggc tcttcagcac gcgcgcgacg ccggcgaagg ccgcgtcggt cagcggcccg
     4141 cgcagccact gcgcggccgg cacctgctcg gccagcgccg ccgcgttggc gggctgctcg
     4201 cgcgaatccc agacgcgcac gtcggcaccg ccgcgggcgc accagcgcgc catcgcgagg
     4261 cccgattcgc cgaggccgag gaccaggacg gtggtgtctt gcaagtcgtt catcatcagc
     4321 gcaacttcag gctcgccaag ccgatcaggc agagcagcat cgtgacgatc cagaaacgca
     4381 cgacgacctg cgtctccttc cagcccgact tctcgaaatg gtggtgcagc ggcgccatct
     4441 tcaggatgcg ccggccggcg ccgtacttct tcttcgtgta cttgaaccac atgacctgga
     4501 ccatcaccga caaggcttcg gcgacgaaca cgccgcccat gatcccgagc acgatctcct
     4561 ggcgcgtgat caccgcgatc gtgccgagcg cgccgcccag cgacagcgcg ccgacgtcgc
     4621 ccatgaagac ctgcgccgga tgcgtgttga accacaggaa cgccaggccc gcaccggcca
     4681 tcgcggcgca gaagatcagc aactcgccgg cgccggggat gtacgggaac agcaggtact
     4741 tgctgtagac cgagctgccg gtgatgtagg cgaagatgcc cagcgccgag ccgaccatca
     4801 cgaccggcat gatcgccagg ccgtcgagtc cgtcggtgag attgaccgcg ttgctcgcgc
     4861 cgacgatcac gaagtaggtc agcgcgatga agccgaacac gccgagcggg tagctgatgg
     4921 tcttgaagaa cggcaccatc aggtcggcct tgggcggcag ctcgttcgcg aagccgctct
     4981 gcacccagcg gatgaagagc tcgagcacgc gcaggttcga ggtctcgctg acgctgaacg
     5041 cgaggtacag cgccgccacg aggccgatca ccgattgcca gaagtacttc tcgcccgagg
     5101 gcatgccttc cgggttcttc cgcaccacct tgcgccagtc gtcgacccag ccgatggcgc
     5161 cgaagcccag cgtgacccac agcacgatcc agacgaagcg gttcgacagg tcgaaccaca
     5221 ggagcgtcgc gaaggcgatc gagaacagca ccagcacgcc gcccatggtg ggcgtgccgg
     5281 ccttggccag gtgcgccttg acgccgtact cgcggatggg ctggccgatc ttcagctcgg
     5341 tgaggcggtt gatcacccag ggaccgaagg ccaggccgat gagcagcgcg gtgagcgagg
     5401 ccatcaccgc ccg
//
LOCUS       DATMJB010000072         2370 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_527676, whole genome shotgun sequence.
ACCESSION   DATMJB010000072 DATMJB010000000
VERSION     DATMJB010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527676"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VNU48_01695"
     CDS             <1..559
                     /locus_tag="VNU48_01695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWI10022.1"
                     /translation="HDGLTRLPNAEYFRTRVDNALARVRQHEQPVAVLFLDLDDFKAI
                     NDTHGHATGDAVLRIVAARLVGGIRAEDLVSRIGGDEFACLLVGVQDRARLSRLVCQM
                     FDSVAGPLTIGRVGLSVRASIGIAMSPADGATSVALMKNADAAMYRAKRQKRGYVFFD
                     EPDDEPASGDAPDRQSSVFGRLALG"
     gene            complement(639..1055)
                     /locus_tag="VNU48_01700"
     CDS             complement(639..1055)
                     /locus_tag="VNU48_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI10023.1"
                     /translation="MSTLKAFVVEDNAVIRESLVAALEELAPVEVIGTAEDERSAVEW
                     LGRSGDAPVCDLMIVDIFLKAGSGLGVLRAARDRHRPIAMVVLSNYATSDMQRRCLDL
                     GASRVFDKSGELEDLIGYCEQLAGHARDGHAVRPPA"
     gene            complement(1212..2288)
                     /locus_tag="VNU48_01705"
     CDS             complement(1212..2288)
                     /locus_tag="VNU48_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HWI10024.1"
                     /translation="MRVIAVEPLRAAVSELMLGFGSAAREAELVADNLIQANLTGHDS
                     HGIGMLPRYANAFLEGGLKPNVHALTALDTGALLALDGQAGFGQVIGHETMTLGIARA
                     QAHGSCIVALANAHHLGRIGAWAEMAVDQGLVSVHFVNVISRPIVAPWGGRDARFGTN
                     PFAVGIPVPGRAPVILDLATSVVAQGKTRVAHNKGEQLAPGQLLDDRGEPTTDPRFGV
                     VEPYGALRTFGEHKGFGLALVCELLGGALAGGLAAQGAADGRQRVLNGMLTILFDPQR
                     LGDAAVWAGEMRAFLDWVVASPPQADTERVRVAGEPERETRAQRLAHGIPVDATTWAE
                     LLAAAAKLGRDPARVHALAGLASM"
BASE COUNT          320 a          865 c          858 g          327 t
ORIGIN      
        1 gcacgacggc ctgacccggc tgccgaacgc cgaatacttc cgcacccgcg tcgacaacgc
       61 gctggcccgg gtgcgacagc acgaacaacc ggtcgcagtg ctgttcctcg acctcgacga
      121 cttcaaggcg atcaacgaca cccacggcca cgccaccggc gacgcggtgc tgcggatcgt
      181 cgcggcgcgc ctggtcggcg ggatccgcgc cgaggacctg gtgagccgga tcggcggcga
      241 cgaattcgcg tgcctgctcg tcggcgtcca ggaccgcgcg cggctgagcc ggctggtctg
      301 ccagatgttc gactcggtgg ccggcccgct gacgatcggc cgggtcggcc tgagcgtccg
      361 ggccagcatc ggcatcgcga tgtcgcccgc ggacggcgcg acctcggtcg cgctgatgaa
      421 gaatgccgac gccgcgatgt accgggcgaa gcgccagaag cgcggctatg tgttcttcga
      481 cgaacccgac gacgaaccgg cgagcggcga cgcaccggac cgtcagtcat cggtcttcgg
      541 ccgcctggcg ctcggctgag agaaagacgc agcgagcggc ccgccgtcag ccgcagcgac
      601 gtgcacctgt cccggcagat gtgagcgccg tgccgcgctc aggccggcgg tctgaccgcg
      661 tggccgtcgc gggcgtggcc ggcgagctgc tcgcagtagc cgatcaggtc ctcgagctcg
      721 cccgacttgt cgaacacccg gctcgcgccc aggtcgaggc agcggcgctg catgtcggac
      781 gtggcgtagt tgctcagcac caccatcgct atcggtcggt gccggtcgcg cgcggcgcgc
      841 agcacgccca ggcccgagcc ggctttcagg aagatgtcga cgatcatcag atcgcacacc
      901 ggggcatcgc cgctgcggcc cagccattcg accgcgctgc gttcgtcctc ggcggtgccg
      961 atcacctcga ccggtgccag ttcctcgagc gccgcgacca ggctctcccg gatcactgcg
     1021 ttgtcttcga cgacgaatgc tttcaatgtg ctcatggccc cgtatcgcgt cgtgtcggcg
     1081 tgccgacacc gacaagcccc ctcacgcaac cgtagcatcc ggcgcggggt cgcgctgtag
     1141 gacgtggcgc tgcgggccgg tctgaggacg ccgtgcagcg gccgcgtggc cgcgccgcgg
     1201 cgggcgcgcc tctacatcga cgcgagcccc gcgagcgcgt gcacgcgggc cggatcgcgg
     1261 ccgagtttcg cggcggcggc gagcagctcg gcccaggtcg tcgcgtcgac cgggatgccg
     1321 tgcgcgagcc gctgcgcgcg ggtctcgcgc tcgggctcgc cggccacgcg cacgcgttcg
     1381 gtgtccgcct gcggcggcga ggcgacgacc cagtcgagga aggcccgcat ctcgccggcc
     1441 cagaccgccg cgtcgcccag gcgctgcggg tcgaacagga tcgtcagcat gccgttgagc
     1501 acgcgctgcc ggccgtcggc cgcgccctgc gccgccaggc cgccggccag cgcgccgccg
     1561 agcagctcgc acaccagcgc gaggccgaag cccttgtgct cgccgaaggt gcgcagcgcg
     1621 ccgtagggct cgaccacgcc gaagcgcggg tcggtggtcg gctcgccgcg gtcgtcgagc
     1681 agctggccgg gtgcgagctg ctcgcccttg ttgtgcgcga cgcgcgtctt gccctgcgcg
     1741 accacgctgg tggccaggtc caggatcacc ggcgcgcgcc ccggcaccgg gatgccgacc
     1801 gcgaacgggt tcgtgccgaa gcgcgcatcg cggccgcccc agggcgcgac gatcggccgc
     1861 gagatcacgt tgacgaagtg caccgagacc agtccctggt ccaccgccat ctcggcccac
     1921 gcgccgatgc ggcccaggtg gtgcgcgttc gccagcgcga cgatgcagct gccgtgggcc
     1981 tgggcgcgtg cgatgccgag cgtcatggtc tcgtggccga tcacctggcc gaagccggcc
     2041 tggccgtcga gcgcgagcag cgcgccggtg tcgagcgcgg tcagcgcatg aacattcggt
     2101 ttcaggccgc cttcgaggaa cgcgttcgca tagcgcggca gcatgccgat gccgtgcgaa
     2161 tcgtggccgg tcaggttggc ctggatcagg ttgtcggcga cgagttcggc ctcgcgcgcg
     2221 gcgctgccga agccgagcat caactcgctc accgcggcgc gcagcggctc gacggcgatg
     2281 acgcgcatcg cggcgctcac tgacgcccct cgcccgatgg gtacatcggc cgatcccccg
     2341 aggggatgag ccttgcttgg ggcggcccgg
//
LOCUS       DATMJB010000073         3194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_522651, whole genome shotgun sequence.
ACCESSION   DATMJB010000073 DATMJB010000000
VERSION     DATMJB010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522651"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VNU48_01710"
     CDS             <1..923
                     /locus_tag="VNU48_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HWI10025.1"
                     /translation="PVAGAGEALIRVGASGVNRPDVLQRKGHYPVPPGASDLPGLEVA
                     GVIEAGDASALAVAGFKLGDRVCALVAGGGYAQLCVAPIGQMLPVPAGLSDVEAASLP
                     ETFFTVWSNVFDRARLKAGETLLIQGGSSGIGVTAIQMAKAAGATVIATAGSDDKCAA
                     CVQLGADHAINYRQSDFVDEVKRLTDGRGADVILDMVAGAYVARELKCLAEDGRLVFI
                     AVQGGVEAQIDAGVVLRKRLTITGSTLRPRPVAFKAAIAASLRATAWPWIEAGKIKPV
                     IYKVFPAAQAAAAHTLMESNQHIGKLVLEW"
     gene            950..1690
                     /gene="tpiA"
                     /locus_tag="VNU48_01715"
     CDS             950..1690
                     /gene="tpiA"
                     /locus_tag="VNU48_01715"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWI10026.1"
                     /translation="MRRKLVVGNWKMNGNRAANAELLAALKAAGPWVAEVAVCAPSPY
                     LGDVALSLQGEAIGWGAQDCSAFESGAYTGEVSAAMLAEFGCRYVLAGHSERRAYHAE
                     SDQLVADKAKAALAHRITPIVCVGETLAEREAGQTDAVVKRQLSAVIHTLGHCIQQIV
                     VAYEPVWAIGTGKTASPEQAQAVHALLRAQLKAATEHAFEMKILYGGSVKADNASSLF
                     AQADIDGGLIGGASLKAGDFAAICRAAG"
     gene            1735..2148
                     /gene="secG"
                     /locus_tag="VNU48_01720"
     CDS             1735..2148
                     /gene="secG"
                     /locus_tag="VNU48_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWI10027.1"
                     /translation="MNLILNLVVALQILTALVMIGLVLIQHGKGADMGASFGSGASGS
                     LFGATGSANFMSRSTAVCAAIFFACTLGLAYFSNDRARPSSGTSVLDRPVVTAPAASV
                     PLSGAALVPGAAPAASAVAAPPAPQASGAGAIPTK"
     gene            2307..2391
                     /locus_tag="VNU48_01725"
     tRNA            2307..2391
                     /locus_tag="VNU48_01725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2341..2343,aa:Leu,seq:gag)
     gene            2485..2844
                     /locus_tag="VNU48_01730"
     CDS             2485..2844
                     /locus_tag="VNU48_01730"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWI10028.1"
                     /translation="MNLEPYLPVILFILIGAAVGVAPQVLGFLLGPNRPDKAKNSPYE
                     CGFEAFEDARMKFDVRYYLVAILFILFDLEIAFLFPWAVSLKEIGAAGFWAMMLFLAI
                     LVVGFAYEWKKGALDWE"
     gene            2887..>3194
                     /gene="nuoB"
                     /locus_tag="VNU48_01735"
     CDS             2887..>3194
                     /gene="nuoB"
                     /locus_tag="VNU48_01735"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HWI10029.1"
                     /translation="MGIEGVLKEGFVTTSVDTLINWSKTGSLWPMTFGLACCAVEMMH
                     AGAARYDIDRFGMLFRPSPRQSDLMIVAGTLCNKMAPALRKVYDQMPEPRYVISMGSC
                     A"
BASE COUNT          493 a         1057 c         1123 g          521 t
ORIGIN      
        1 acccggtcgc gggtgcgggc gaggcactga tccgcgtcgg cgcctcgggg gtgaaccggc
       61 cggatgtgct gcaacgcaag ggtcactacc cggtgccgcc gggcgcgtcc gacctgccgg
      121 gcctggaggt cgcgggcgtc atcgaggccg gcgacgcgtc ggcgctcgcg gtggctggct
      181 tcaagctcgg cgaccgcgtc tgcgcgctgg tcgccggtgg cggctacgcg caactgtgcg
      241 tcgcgccgat cggccagatg ctgccggtgc cggcgggcct gagcgacgtc gaggccgcga
      301 gcctgcccga gaccttcttc accgtctggt cgaacgtgtt cgaccgtgcg cgcctgaagg
      361 ccggcgaaac gctgctgatc cagggcggca gcagcggcat cggcgtgacg gcgatccaga
      421 tggccaaggc agccggcgcg acggtgatcg ccaccgccgg cagcgacgac aaatgcgccg
      481 cctgcgtgca actcggtgcc gaccacgcga tcaactaccg ccagtccgat ttcgtcgacg
      541 aggtcaagcg cctcaccgac ggccgcggcg ccgacgtgat cctcgacatg gtcgccggcg
      601 cctacgtggc gcgcgagctg aagtgcctgg ccgaagacgg ccgcctggtg ttcatcgcgg
      661 tgcagggcgg cgtcgaggca cagatcgatg ccggtgtcgt gctgcgcaag cgcctgacga
      721 tcaccggctc gacgctgcgg ccgcggccgg tggcgttcaa ggcggcgatc gccgcgtcgc
      781 tgcgcgcgac ggcctggcca tggatcgagg ccggcaagat caagccggtg atctacaagg
      841 tgttcccggc cgcgcaggcc gcggccgcgc acacgctgat ggaatcgaac cagcacatcg
      901 gcaagctggt gctggagtgg tgacgatgaa gatctcggaa acctcggcgg tgcgccgcaa
      961 actcgtcgtc ggcaactgga agatgaacgg caaccgcgcc gcgaatgccg agctgctggc
     1021 ggcgctgaag gcggccggcc cctgggtcgc ggaagtggcg gtgtgcgcgc ccagccccta
     1081 cctcggcgac gtggccctgt cgctgcaggg cgaggccatc ggctggggtg cgcaggactg
     1141 ttcggcgttc gagtcgggcg catacaccgg cgaggtgtcg gcggcgatgc tcgccgagtt
     1201 cggctgccgt tacgtgctgg ccggccactc cgagcgccgc gcgtaccacg ccgagagcga
     1261 tcagctcgtc gccgacaagg cgaaagccgc gctcgcgcac cgcatcacgc cgatcgtgtg
     1321 cgtcggcgaa acgctggccg agcgcgaggc gggccagacc gacgccgtcg tcaagcgcca
     1381 gctgtcggcg gtgatccaca cgctgggcca ctgcatccag cagatcgtcg tcgcctacga
     1441 gcccgtctgg gcgatcggca ccggcaagac cgcctcgccc gagcaggcgc aggcggtgca
     1501 tgcgctgctg cgcgcgcagc tgaaggcggc gaccgaacac gcgttcgaga tgaagatcct
     1561 gtacggcggc agcgtcaagg ccgacaacgc ctcgagcctg ttcgcgcagg ccgacatcga
     1621 cggcggcctg atcggcggtg cttcattgaa ggcgggcgat ttcgccgcca tctgccgcgc
     1681 agccggttga ttcggtcgac cgccgcgcaa cgagagaatt ttggagtccg aagaatgaac
     1741 ctgatattga acctggtcgt ggcgctgcag atcctcaccg cgctggtgat gatcggcctg
     1801 gtgctgatcc agcacggcaa gggcgccgac atgggcgcgt cgttcggcag cggcgcgtcg
     1861 ggcagcctgt tcggtgccac cggcagcgcg aacttcatgt cgcgctcgac cgcggtgtgc
     1921 gccgcgatct tcttcgcctg cacgctcggg ttggcgtact tttcgaacga tcgcgcgcgg
     1981 ccgtccagcg gcaccagcgt gctcgaccgg ccggtcgtca cggcaccggc agccagtgtg
     2041 ccgctcagcg gcgccgcgct ggtgccgggc gccgcaccgg ccgcctccgc ggtcgctgca
     2101 ccgccggcgc cgcaggcatc gggcgcgggc gcgatcccga ccaagtaacg cgaccctcgc
     2161 gagccccggt tgcggggcgt cgcgcacctc gtggcggtgc agccggcgtc aaggaacagc
     2221 aaggctttga ggttagaatg cgcagctgtc cggtgagcca ctcctcttgc gatccggtcc
     2281 ctgcttcaag tgcttttttg tgcttggccg tcgtggtgaa attggtagac acgctatctt
     2341 gagggggtag tggcgaaagc tgtgcgagtt cgagtctcgc cgacggcacc aaaattgctc
     2401 ggcccctttg cgcgatgctc cgcgtcgcgt ttcgaggtcg atccgctcca gctacagcag
     2461 gctctcgcgg gcccgcaatg accgatgaat ctggaaccct acctgcccgt catcctgttc
     2521 atcctgatcg gtgcggcagt cggcgtggca ccccaggtgc tcggattcct gctcggtccg
     2581 aaccggcccg acaaggcgaa gaactcgccc tacgaatgcg gcttcgaggc cttcgaagac
     2641 gcgcgcatga agttcgacgt gcgctactac ctcgtcgcga tcctgttcat cctgttcgac
     2701 ctcgagatcg cgttcctgtt tccgtgggcg gtgtcgctca aggagatcgg cgcggcgggc
     2761 ttctgggcca tgatgctgtt cctggcgatc ctggtcgtgg gcttcgcgta cgagtggaag
     2821 aagggcgcac tcgattggga atgacggtcg atcgaacgcg cacaggcatc accacagagc
     2881 agcaccatgg gcattgaagg cgtactgaaa gagggcttcg tcaccaccag tgtcgacacc
     2941 ctgatcaact ggtcgaagac cggttcgctg tggccgatga ccttcggcct cgcgtgctgc
     3001 gcggtcgaga tgatgcacgc cggtgcggcg cgctacgaca tcgaccgttt cggcatgctg
     3061 ttccggccca gcccgcggca gagcgatctg atgatcgtcg ccggcacgct gtgcaacaag
     3121 atggcgccgg cgctgcgcaa ggtctacgac cagatgccgg agccgcgcta cgtcatctcc
     3181 atggggtcgt gcgc
//
LOCUS       DATMJB010000074         3196 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_581697, whole genome shotgun sequence.
ACCESSION   DATMJB010000074 DATMJB010000000
VERSION     DATMJB010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581697"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VNU48_01740"
     CDS             <1..1274
                     /locus_tag="VNU48_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012434480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI10030.1"
                     /translation="ALKSSPTLHLTDAQIGASASLYLVGAVTGALVFGALADRFGRRR
                     LFSVTLGLYLVATALTAFSHDFFSFALWRMLTGAGIGGEYSAINSAIQELIPARYRGR
                     TDLIVNGSFWIGAALGAGGAVVLLAPGRLPPDWGWRCAFGIGAVLGLAILYLRRFMPE
                     SPRWLMTHGRVAEAELVLARIEGAAGVAAPDGTLTRIRLAPRRVGLVEVARTLLRTYP
                     RRSIHVLVLMASQAFFYNAIFFTYALVLGRFFGVADDRIGLYILPFAAGNFLGPLLLG
                     PAFDVIGRRPMIAITYAASGVLLAGTALLFVGGVLGATGMTVAWSVVFFFASAAASAA
                     YLSAGESFPLEMRALAIAIFYAVGTALGGVAGPWLFGVLIGSGERGAIAWGYAFGALL
                     MLVAAVVTLKLGVAAERRPLEDVARPLSSAS"
     gene            1388..1948
                     /locus_tag="VNU48_01745"
     CDS             1388..1948
                     /locus_tag="VNU48_01745"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWI10031.1"
                     /translation="MTTTRPDPMKLFALEHSRGAYCADFVLYSAAVVVLTAVLALAQP
                     LAQWPALALLGLAGWTLVEYAMHRFVLHGLQPFKAWHARHHERPTARIASPTILSAGL
                     IFTLVFLPAFALAGGWNACALTLGILIGYLGYAITHHATHHWNVDNAWLKRRKRAHAL
                     HHHLARPGYFGVTSTLWDHVFGSARR"
     gene            2014..>3196
                     /locus_tag="VNU48_01750"
     CDS             2014..>3196
                     /locus_tag="VNU48_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HWI10032.1"
                     /translation="MLDPLVRRLAAAAAAALALFALVGCGTLPEIDRDAIASEAIPLS
                     TKTTLGRIAKTSQPAPELSGFRLMPLGLFSLDTRVQLARRAEVSLDVQYYHFENDETG
                     RWLLRALRDAAERGVRVRVLIDDLYTGGQDDYWRAFAAHKNVQVRLFNPFCCARGQGQ
                     TERFIASANEWSRVNHRMHNKLFIADGAMAVIGGRNVANEYFLRGMTENFVDVDAFAV
                     GWVVPPLQFHFDRYWNSAAVYPLEAIAKTALNPAELRARFEEWTGPVRTPPPQELPPN
                     DVLGYGPIADDLDGGRLGLIWGQAFVFADHPDKPFDGSVGGEPLDTSVTYNLFEVVRA
                     AKKEVVMVSPYFVPGELSLQMLRDLRGKGVAVTVMTNSLASTDEPAVHIGYAAHREEL
                     LR"
BASE COUNT          409 a         1144 c         1122 g          521 t
ORIGIN      
        1 gggcgctgaa gtcgagcccg acgctgcacc tcaccgatgc gcagatcgga gcgtcggcaa
       61 gtctgtacct ggtcggtgcc gtcaccggcg cgctggtgtt cggcgcactg gccgaccgtt
      121 tcggccgccg gcgcctgttc agcgtcacgc tcggcctgta cctggtggcg accgcgctga
      181 ccgcgttctc gcacgacttc ttcagcttcg cgctgtggcg catgctgacc ggcgcgggca
      241 tcggcggcga gtattcggcg atcaactcgg cgatccagga gttgatcccg gcgcgctacc
      301 gcggccgcac cgacctgatc gtcaacggca gcttctggat cggcgccgcg ctcggcgccg
      361 gcggcgcggt cgtgctgctg gcaccgggcc ggctgccgcc cgactggggc tggcgctgcg
      421 cgttcggcat cggcgcggtg ctggggctgg ccatcctgta cttgcggcgc ttcatgccgg
      481 agagcccgcg ctggctgatg acccacgggc gcgtcgccga ggccgaactc gtgctggcgc
      541 ggatcgaggg cgccgccggc gtcgcggcgc cggacggaac gctgacgcgc atccgcctcg
      601 cgccgcgacg ggtgggcttg gtcgaggtcg cgcgcacctt gttgcgcacc tacccgcggc
      661 gctcgatcca cgtcctggtg ctgatggcct cgcaggcctt cttctacaac gccatcttct
      721 tcacgtacgc gctcgtgctc gggcgattct tcggtgtcgc cgacgaccgg atcggcctgt
      781 acatcctgcc gttcgccgcc ggcaacttcc tcgggccgct gctgctcggg cccgcgttcg
      841 acgtgatcgg acgccggccg atgatcgcga tcacctacgc ggcctctgga gtgctgctcg
      901 ccggcacggc gctgttgttc gtcggcggcg tgctgggcgc gaccggcatg acggtggcgt
      961 ggtcggtggt gttcttcttc gcatcggcgg cggcgagtgc cgcctacctg agcgccggcg
     1021 agagctttcc gctcgagatg cgcgcgctgg ccatcgccat cttctacgcc gtcggcaccg
     1081 cgcttggcgg cgtcgccggg ccgtggctgt tcggcgtgct gatcggcagc ggcgagcgcg
     1141 gcgcgatcgc ctggggctac gcgttcggcg cgctgctgat gctggtcgct gcggtggtca
     1201 cgctcaagct cggcgtcgcc gccgagcgtc ggccgctcga ggatgtcgcg cgtccgttgt
     1261 cgagcgccag ctagcctcag agcctgagag gttttcgggc gcgcccgagc gggcgcgtgc
     1321 ccacccggca ctgggatatg cacgagccgc ccgaacccga aacggcgaac ccgtgtccaa
     1381 tgcggccatg accaccaccc gacccgaccc gatgaagctt tttgcgctgg aacacagccg
     1441 gggcgcgtac tgtgccgact tcgtgctcta cagtgccgcc gtcgtcgtgc tgaccgccgt
     1501 gctggcgctc gcgcagccac tcgcgcagtg gccggcgctg gcgctgctgg gcctggcggg
     1561 ctggacgctc gtcgagtacg cgatgcaccg cttcgtgctg cacggcctgc agccgttcaa
     1621 ggcctggcat gcgcgccacc acgagcggcc gacggcgcgg atcgcgagcc cgacgatcct
     1681 cagcgccggg ctgatcttca cgctggtgtt cctgccggcg ttcgccctgg ccggcggctg
     1741 gaacgcctgc gccctgacgc tgggcatcct gatcggctac ctcggctatg cgatcaccca
     1801 ccatgccacg caccactgga atgtcgacaa cgcctggctg aagcggcgca agcgcgcgca
     1861 tgcgctgcac catcacctgg cgcggcccgg ctacttcggc gtcaccagca cgctctggga
     1921 ccacgtgttc ggcagcgcgc ggcgttgacc ggtgcctgct tgcgtcgcgt ggcaatatcc
     1981 gcgggtcccg aacgactcac gcagatgacc cggatgctcg accccctcgt gcgccgcctc
     2041 gcggccgcgg ccgccgcggc gctggcgctg ttcgcgctgg tcggctgcgg caccttgccc
     2101 gagatcgacc gcgacgcgat cgcgagcgag gcgatcccgc tgagcacgaa gaccacgctc
     2161 ggccggatcg ccaagacctc gcagcccgcg cccgagctgt ccggcttccg gctgatgccg
     2221 ctggggctgt tctcgctcga cacgcgggtg cagctggcca ggcgcgccga ggtctcgctc
     2281 gacgtgcagt actaccactt cgagaacgac gagaccggcc gctggctgct gcgcgcgctg
     2341 cgcgacgcgg ccgagcgcgg cgtgcgcgtg cgcgtgctga tcgacgacct ctacaccggc
     2401 gggcaggacg actactggcg cgccttcgcg gcccacaaga acgtgcaggt gcggctgttc
     2461 aacccgttct gctgcgcgcg cgggcagggc cagaccgagc gcttcatcgc gtcggcgaac
     2521 gaatggtcgc gcgtcaacca ccgcatgcac aacaagctgt tcatcgccga tggcgcgatg
     2581 gcggtgatcg gcgggcgcaa tgtcgcgaac gagtacttcc tgcgcggcat gaccgagaac
     2641 ttcgtcgatg tcgacgcgtt cgcggtcggc tgggtcgtgc cgccgctgca gttccacttc
     2701 gaccgctact ggaacagcgc cgcggtctac ccgctcgagg cgatcgcgaa gaccgcgctg
     2761 aacccggccg agctgcgcgc gcgcttcgag gaatggaccg ggccggtgcg cacgccgccg
     2821 ccgcaggaac tgccgccgaa cgacgtgctc ggctacgggc ccatcgccga cgacctcgac
     2881 ggcggccggc tcggcctgat ctggggccag gccttcgtgt tcgccgacca ccccgacaag
     2941 cccttcgacg gcagcgtcgg cggcgagccg ctggacacca gcgtgaccta caacctgttc
     3001 gaggtggtcc gcgccgccaa gaaagaggtc gtgatggtct cgccgtattt cgtgcccggc
     3061 gagctgagcc tgcagatgct gcgcgacctg cgcggcaagg gcgtggcggt gacggtgatg
     3121 acgaactcgc tcgcgtcgac cgacgagccg gccgtgcaca tcggctacgc ggcgcaccgc
     3181 gaagagttgc tgcgcc
//
LOCUS       DATMJB010000075         4105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_640955, whole genome shotgun sequence.
ACCESSION   DATMJB010000075 DATMJB010000000
VERSION     DATMJB010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640955"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VNU48_01755"
     CDS             <1..1083
                     /locus_tag="VNU48_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HWI10033.1"
                     /translation="ASLLLVVFSIGIGTGSLLCEMLSKRHVEIGLVPMGAIGMSVFAI
                     DLYFASNGLPPADALALRAFVSAPAHWRVMADLALLSLFAGLYSVPMYALIQLRSQAS
                     HRARIIAANNILNALFMIVSAIGVGALLAAHFTIPQVFLITGLLNAVVAFYIFMLVPE
                     YFLRFVAFIATRLVYRFRVRGDEHIPTEGAAILVCNHVAFVDPVLLMAASPRPIRFIM
                     DHKIFAMPLLGWFFRLAKAIPIAPQREDPVAYEQAFVRARQVLAEGDLLCIFPEGALT
                     RDGELGEFKGGVMKLLETNPVPVVPLALQNLWGSYFSRHGGAAMSRPFRRGWFSRVAL
                     VAGDAVAPGTVTPAGLRERVSHLLAS"
     gene            1111..1791
                     /locus_tag="VNU48_01760"
     CDS             1111..1791
                     /locus_tag="VNU48_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HWI10034.1"
                     /translation="MALQLNRARLARLVPFAVFMLLLAARGAFPEDGSWGIDPRWIYA
                     VAVLVVGALLGWFWRDYGELVPQTWPSGPEIALAVVSGLVVFALWIQLDARWMTIGSP
                     TATFLPLDAQGALIWPLVIARCVGAVLVVPVMEELFWRSFVMRWIQSHQFESVVPQQV
                     GLKAVVLSTFVFTLAHTLWLAAVLAGLVYAWLYLRTGRLWVPVIAHAVTNGALGVWVI
                     ATGNWSFW"
     gene            complement(1760..2656)
                     /locus_tag="VNU48_01765"
     CDS             complement(1760..2656)
                     /locus_tag="VNU48_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWI10035.1"
                     /translation="MLRHPGAFALGTLKAFRANQGLLLAGAVAYYALLSIVPLLILIV
                     IALSHVVDQGALLETLGRYLEWLVPGQSRAIVAELSNFLAHRELVGWVLGVTMLFFSS
                     LAFTVLENAMSVIFLHRVVDWSRHPLISIVMPYLYILSLGIGLLLVTLVAGSLQAIGQ
                     ESVDFLGRRWSLHGVSGALLYLLGVAGEIFVLTSVYLVMPVGRLRISRALLGGVTAAV
                     LWEITRHVLVWYFATLSQVSVVYGSLTTAIVVLLSLEIAATLLLFGAQVIAEYERLGH
                     PHAAPAGAWAAGVTRTTSSPSR"
     gene            complement(2802..3227)
                     /locus_tag="VNU48_01770"
     CDS             complement(2802..3227)
                     /locus_tag="VNU48_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWI10036.1"
                     /translation="MATINDIMTREVSTIQLSDALQTAAQRMRDLNVGVLPVCDGETL
                     VGMVTDRDIAVRGVAAGLNPADALVSDVMTDEVHSCSATDTIDAAMKQMGGAQVRRLA
                     VLDPKRRIVGIVTLGDLATRQSAPTDAALREISESGPTV"
     gene            complement(3367..>4105)
                     /gene="tgt"
                     /locus_tag="VNU48_01775"
     CDS             complement(3367..>4105)
                     /gene="tgt"
                     /locus_tag="VNU48_01775"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWI10037.1"
                     /translation="VLDSDIVMQFDECTPYETKGHVTTEHEARASMELSLRWAQRCLA
                     EHARLENPNALFGIVQGGMFENLREESLGKLVELDLPGYAVGGVSVGEPKPLMQRIVA
                     HTPHRLPAHKPRYLMGVGTPEDLVDGVAAGVDMFDCVMPTRNARNGHLFTRFGDLKIR
                     NARHKLDERPLDPTCGCSTCASFSRAYLHHLDRCGEMLAPMLASVHNLHYYVNLMREL
                     REALDAGTFAEFARRFREDRGRGIALG"
BASE COUNT          582 a         1419 c         1437 g          667 t
ORIGIN      
        1 gcctcgctgc tgctggtggt gttctcgatc ggcatcggca ccggctcgct gctgtgcgag
       61 atgctgtcca agcgccatgt cgagatcggc ctggtgccga tgggcgcgat cggcatgagc
      121 gtgttcgcga tcgacctgta cttcgcctca aatggcctgc cgccggccga cgctctggcg
      181 ctgcgtgcgt tcgtgagcgc gcccgcgcac tggcgtgtga tggccgatct ggcgctgctg
      241 tcgctgttcg cggggctgta cagcgtgccg atgtacgcgc tgatccagct gcgctcgcag
      301 gcgagccacc gtgcccgcat catcgcggcg aacaacatcc tgaatgccct gttcatgatc
      361 gtcagcgcga tcggcgtcgg cgcgctgctg gcggcccatt tcacgatccc gcaggtgttc
      421 ctgatcaccg ggctgctcaa cgcggtggtc gcgttctaca tcttcatgct ggtgccggaa
      481 tacttcctgc gcttcgtcgc gttcatcgcg acaaggctgg tctatcgctt cagggtgcgc
      541 ggcgacgagc acatcccgac cgagggcgcg gcgatcctgg tgtgcaacca cgtggcgttc
      601 gtcgacccgg tgctgctgat ggcggccagc ccgcggccga tccgcttcat catggaccac
      661 aagatcttcg cgatgccgct gctgggctgg ttcttccggc tcgccaaggc gatcccgatc
      721 gcgccgcagc gcgaggaccc ggtcgcctac gaacaggcct tcgtgcgcgc gcgccaggtg
      781 ctcgccgagg gcgacctgct gtgcatcttc cccgagggcg cgctgacccg cgacggcgaa
      841 ctcggcgagt tcaagggcgg cgtgatgaag ctgctcgaaa cgaacccggt gccggtcgtg
      901 ccgctggcgc tgcagaacct gtggggctcg tacttctcgc gccacggcgg ggccgcgatg
      961 agccggccgt tccggcgcgg ctggttcagc cgcgtcgcgc tcgtggccgg cgacgcggtg
     1021 gcgccgggca cggtcacgcc ggccgggctg cgcgagcggg tttcgcatct tctggcatcc
     1081 tgagcgcctt ttgccaatcc cagggaccgc atggccttgc agttgaaccg cgcccgactg
     1141 gcgcgcctcg tgccgttcgc cgtgttcatg ctgctgctcg cggcgcgcgg cgctttcccg
     1201 gaggacggct cgtggggcat cgacccgcgc tggatctacg ccgtggccgt gctcgtggtc
     1261 ggcgccctgc tcggctggtt ctggcgcgac tacggcgagc tcgtcccgca gacctggccg
     1321 agcgggcccg agatcgcgct ggcggtcgtg agcggtctcg tcgtgttcgc gctgtggatc
     1381 cagctcgacg cgcgctggat gacgatcggc agcccgaccg cgaccttcct gccgctggac
     1441 gcgcagggcg cgctgatctg gccgctggtc atcgcccgct gtgtcggcgc ggtgctggtc
     1501 gtgccggtga tggaggagct gttctggcgc tcgttcgtga tgcgctggat ccagagccat
     1561 cagttcgaat cggtcgtgcc gcagcaggtc gggctgaagg cggtggtgct gtcgaccttc
     1621 gtgttcacgc tcgcccacac cctgtggctc gcagcggtgc tcgccggcct cgtctacgcc
     1681 tggctgtacc tgcgcaccgg ccggctgtgg gtgccggtga tcgcgcacgc ggtgacgaac
     1741 ggtgcgctgg gcgtgtgggt catcgcgacg gggaactggt cgttctggtg acgcccgccg
     1801 cccaggcgcc ggccggtgcg gcatgcggat ggcccaggcg ttcgtattcc gcgatcacct
     1861 gcgcgccgaa cagcagcagc gtggcggcga tctccagact cagcagcacg acgatcgcgg
     1921 tggtcagcga gccgtagacc acgctgacct gcgacagggt cgcgaagtac cagaccagca
     1981 cgtggcgcgt gatctcccac aacaccgccg ccgtcacgcc gccgagcagc gcgcgactga
     2041 tgcgcagccg ccccaccggc atcaccaggt agaccgaggt cagaacgaag atctcgccgg
     2101 ccacgccgag caggtacagc agcgcgcccg acacgccgtg cagcgaccag cgccgcccga
     2161 ggaagtcgac gctttcctgg ccgatcgcct gcaggctgcc ggccaccagc gtgaccagca
     2221 gcaggccgat cccgagcgac aggatgtaca gatacggcat cacgatcgag atcagtgggt
     2281 ggcggctcca gtcgacgacg cggtgcagga agatcaccga catcgcgttc tcgagcacgg
     2341 tgaacgcgag cgaactgaag aacagcatcg tcacgcccag cacccagccg accagctcgc
     2401 ggtgcgccag aaaattcgac agctcggcga cgatcgcacg cgactggccc ggcaccagcc
     2461 actcgaggta gcggcccagc gtctccagca gcgcgccctg gtcgacgaca tgcgacagcg
     2521 cgatcacgat caggatcagc agcggcacga tcgacagcag cgcgtagtac gcgaccgcgc
     2581 cggccagcag caggccctgg ttggcgcgaa acgccttcaa ggtgccgagc gcgaacgcgc
     2641 cgggatggcg cagcagttcg cgcgcttgat gacggccccc agcgtcgctt cgctcctgcc
     2701 ccccgagggg gtgctcagcc gccttggggc ggcccggcgg cggctgagaa ggggcgatcg
     2761 gccgcgcgcg ctgcgcgggc ggggccgcgt gtctcacggc atcagaccgt cgggccgctt
     2821 tccgagatct cgcgcagcgc ggcgtcggtc ggcgcgctct ggcgcgttgc caggtcgccg
     2881 agcgtgacga tgccgacgat ccggcgcttt gggtccagca ccgccaggcg acgcacctgc
     2941 gcgccgccca tctgcttcat cgcggcgtcg atcgtgtccg tggcgctgca cgaatgcacc
     3001 tcgtcggtca tcacgtcgga caccagcgcg tcggccgggt tcagccccgc ggcgacgccg
     3061 cgcaccgcga tgtcgcggtc ggtcaccatg cccaccagcg tctcgccgtc gcacaccggc
     3121 agcacgccga cgttcagatc gcgcatgcgc tgtgccgcgg tctgcagcgc atcggagagc
     3181 tggatggtgg acacctcgcg ggtcatgatg tcgttgatcg ttgccatgac ggtgctcctg
     3241 tgaaggcggc ggccgagcga cgactgttgg ccggccccac ccgccacagg ctaggggcgc
     3301 cccacgggac gggccatcgg gagacgacgg cccgcggcgt aagacttctc cgacgccgcc
     3361 ggcgcgctac ccaagcgcga tgccgcgccc gcggtcctcg cggaagcgcc gcgcgaattc
     3421 ggcgaaggtt ccggcgtcga gcgcctcgcg cagctcgcgc atcaggttca cgtagtagtg
     3481 caggttgtgc acgctggcca gcatcggcgc gagcatctcg ccgcagcgat cgaggtggtg
     3541 caggtaggcg cgcgagaagc tcgcgcaggt gctgcagccg caggtcgggt cgagcgggcg
     3601 ctcatccagc ttgtggcgtg cgttgcggat cttcaaatcg ccgaagcgcg tgaacagatg
     3661 gccattgcgc gcgttgcgcg tcggcatcac gcagtcgaac atgtcgacgc cggcggccac
     3721 gccgtcgacc aggtcctcgg gcgtgcccac gcccatcagg tagcgcggct tgtgcgctgg
     3781 caggcggtgc ggcgtgtgcg cgacgatgcg ctgcatcagc ggcttgggtt cgccgacgct
     3841 gacgccgccg accgcataac cgggcaggtc gagttcgacc agcttgccga gcgactcctc
     3901 gcgcaggttc tcgaacatgc cgccctgcac gatgccgaag agagcattcg ggttctcgag
     3961 ccgcgcgtgc tcggccaggc agcgctgcgc ccagcgcagg ctcagctcca tcgacgcgcg
     4021 ggcctcatgc tcggtcgtca catggccctt ggtctcgtag ggcgtgcact cgtcgaactg
     4081 catcacgatg tcggagtcga gcacc
//
LOCUS       DATMJB010000076         2236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_404800, whole genome shotgun sequence.
ACCESSION   DATMJB010000076 DATMJB010000000
VERSION     DATMJB010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404800"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..743)
                     /locus_tag="VNU48_01780"
     CDS             complement(105..743)
                     /locus_tag="VNU48_01780"
                     /inference="COORDINATES: protein motif:HMM:NF038119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHFG family PEP-CTERM protein"
                     /protein_id="HWI10038.1"
                     /translation="MIDACTWNAGADRYTGDVPAAIAAYGLPLATQSALIRAFERREF
                     VDAVVIDREAIRGKTAEYDSRIRAMHFGSRGRICAEVTRTGWAADHVETALVLCADGE
                     CIAWPAVCGNVFRITRHERAVADEIVPAELAADAMSDLAPEAQPLVLREISPEPAAGA
                     TAAPGEPGWMSWPEPMPAPWIRAPHTPPIPEPSTWALFVAGALALALRRVRR"
     gene            complement(740..985)
                     /locus_tag="VNU48_01785"
     CDS             complement(740..985)
                     /locus_tag="VNU48_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10039.1"
                     /translation="MNQTRLGSLIEALFNVAIGLAVSVVANALVFPRFDFHPSLGQNV
                     AISVIYTVISIVRSYCVRRWFNARLQSAAARLARAAS"
     gene            complement(996..1517)
                     /locus_tag="VNU48_01790"
     CDS             complement(996..1517)
                     /locus_tag="VNU48_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10040.1"
                     /translation="MFGQNIREQAAQWPTPAQRDGDSRRGATSPDSDAWKNKVGRGAV
                     NAAGMLSDDLKSSAVAWPTPRARDHKGGGTAVTRPDGKSRMDMLDWRAEAFSRQVLPT
                     PVGQESSPSTPNSPRHLNPVFGAWLMGWPSTWVIAEPHASSALATESWRRALQRQLSS
                     LLGEPACSPLGLP"
     gene            complement(1570..2082)
                     /locus_tag="VNU48_01795"
                     /pseudo
     CDS             complement(1570..2082)
                     /locus_tag="VNU48_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
BASE COUNT          375 a          775 c          733 g          353 t
ORIGIN      
        1 tttagcggct tgaaagtcga acaggaaatc aggcatccag aggggctcga aacccatgtc
       61 tgcacctgtg tgttccttct cgcgcaagaa cgtggtgtag tcgctcatcg acgcaccctc
      121 cgcagcgcaa gggcaagcgc accggcgacg aacagcgccc aggtcgatgg ctcggggata
      181 ggcggcgtgt gcggggcgcg gatccacggc gccggcatgg gctcaggcca cgacatccag
      241 ccgggttcgc cgggcgcggc ggtggcgcct gctgcgggct ccggcgagat ctcgcgcagc
      301 acaagcggct gcgcttccgg cgccaggtcg gacattgcat cagcagccag ctcggccggc
      361 acgatctcgt cagcaactgc gcgctcgtgc ctcgtgatcc ggaacacgtt cccgcacacc
      421 gccggccacg cgatgcactc gccgtcggcg cacagcacca gcgcggtctc gacgtggtcg
      481 gcagcccagc cggtgcgcgt gacttctgcg cagatgcggc cgcgcgagcc gaagtgcatc
      541 gctcggatcc ggctgtcgta ctcggcggtc ttgccgcgga tggcctcgcg gtcaatgacg
      601 acggcgtcga cgaactctcg gcgttcgaac gcgcggatca gcgcgctctg cgtggccagg
      661 ggcaggccgt aggctgcgat cgccgcgggc acgtcaccgg tatacctgtc ggcgccggcg
      721 ttccatgtgc aggcgtcgat cacgatgcag ccctcgcgag gcgcgctgca gctgactgca
      781 gccgcgcgtt gaaccatcga cgcacgcagt acgaacgcac gatgctgatc acggtgtaga
      841 tgacgctgat cgcgacgttc tggcccagcg acgggtggaa gtcgaagcgc gggaagacca
      901 acgcgttcgc aaccacgctg acggccaggc cgatggcgac gttgaacagg gcttcgatca
      961 gcgagccgag gcgggtctgg ttcatgccgt ggctttcagg gcaggccgag cggcgaacag
     1021 gccggctcgc cgagcaagga tgacaactgc cgttgcagcg cacgccgcca cgactccgtt
     1081 gccaacgcac ttgaggcgtg cggctcggct atcacccatg tcgaaggcca gcccatcaac
     1141 cacgcaccga aaaccgggtt caagtgccgg ggcgagttcg gggtgctcgg cgatgattcc
     1201 tgcccaacgg gtgtcggcag gacctggcgc gaaaatgcct ctgctcgcca gtccagcatg
     1261 tccatccggc tcttgccatc cgggcgcgtc acggctgtgc cccccccttt gtggtcgcgc
     1321 gctctgggcg tcggccaggc cactgccgac gatttcagat cgtccgagag catccccgct
     1381 gcgttcaccg cgccgcggcc gaccttgttc ttccaagcat ccgaatccgg gctggtcgca
     1441 ccgcggcgcg agtccccgtc ccgttgcgct ggagtcggcc actgcgccgc ctgttcccgg
     1501 atgttctgcc cgaacacgcc gttgctgtag ctcgaggtgt tgcagtctgg tgtggcccac
     1561 gcgccaggcg agacagaacc atcgggcgcg cccgtgactg gcgcccacgt cgctcgcgga
     1621 aagagtgacc cactccgcat cccagccgag gtcggccagt tctcccacga cgcgggcagc
     1681 cgcgcgctcg tcgagctcgc cttcggcctc gtccacaacg gaggcggtgg cagaagcgat
     1741 gcctgcgacg ttctccagaa gcatgagcca cgcaccgcta tcggttgcga tccgaacgac
     1801 ctcgaagaac aggccgctgc gcttgccgtc gagcccggcg cgtcggccgg cgacgctgag
     1861 gtcttggcac gggaaaccgg caacgatgca atccacgcgg ccatgccacg cgccagcgtc
     1921 gaaggtgcaa acgtcggacc agataggtgc cgcatccatg ctgccttctt ccatgcgcgc
     1981 cgccaggacg ccggctccat gtgcttcccg ctcaacgtgg caaacggtgc gatacgcgac
     2041 acccatatgg gcgagcccgg ctcgcagtcc ttctccaagc atgccgacgc ctgagaaaag
     2101 ctcgatggaa tgaagaccca catcaggcag cgattgcacg gatcacctcg cacggcgtct
     2161 cgttcggccg ccccgccagc cgcagcacct cgtcgacgcc gtcggcatcg cggatcggca
     2221 tcaggcagtt gtcgtt
//
LOCUS       DATMJB010000077         2505 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_403986, whole genome shotgun sequence.
ACCESSION   DATMJB010000077 DATMJB010000000
VERSION     DATMJB010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403986"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1451
                     /gene="cysS"
                     /locus_tag="VNU48_01800"
     CDS             78..1451
                     /gene="cysS"
                     /locus_tag="VNU48_01800"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HWI10041.1"
                     /translation="MTLRIHNTLSRELETFTPIEPNHVRMYVCGITIYDLCHVGHARM
                     MVAFDLIYRWLRVLGYRVTYVRNVTDIDDKIIRRALERQIPIRQLTDEMIIAMHTDLD
                     ALGIERPTHEPRATEYVPQMLAMIGTLEAKGLAYRSGNGDVNYAVRKFAGYGNLSGKS
                     VDELRSGERVAVLDGKDDPLDFVLWKAAKAEEPDDAKYPSGYGAGRPGWHIECSAMAC
                     ALLGEQFDLHGGGADLQFPHHENEIAQSEGATGRPPARLWAHNGLLNVDQVKMSKSLG
                     NFFTIREVLARYDAETIRFFMLRTHYRSPFNFSDANLDDARASLRRLYTALDGLDVPV
                     QPIDWAAPQAAAFRAAMNDDFNTPLAVAVLFELAAEANRTRSLEAAALLKGLAATLGF
                     LQQVPRSYLQGGNTLDEAHIAERIGARNAAKKAGDFALADAIRAELLAQGIVLQDSAG
                     GTTWVKA"
     gene            1451..2143
                     /locus_tag="VNU48_01805"
     CDS             1451..2143
                     /locus_tag="VNU48_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HWI10042.1"
                     /translation="MPARAVQPELPATPGLAAAARITPDYWDDACKHLSRRDRVMRKL
                     IPRLGEGRLQSRGDAFTTLARSIVGQQISVAAAQAVWTRFAALMSSPSTRLAPTEVLA
                     LETSAARAAGLSARKVEYLCDLAAHFESGAVHVDQWQGMDDEAIIDELVAIRGIGRWT
                     AEMFLIFHLMRPNVMPLDDLGLLKGISVNYFSGEPVSRAEAREVGDAWAPFRSVATWY
                     IWRSLDPLPVDY"
     gene            2181..>2505
                     /locus_tag="VNU48_01810"
     CDS             2181..>2505
                     /locus_tag="VNU48_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HWI10043.1"
                     /translation="MSKRHFLEFEQPVSELESKIEELRYVQSESAVDISEEIDRLGKK
                     SLQLTKDIYSSLTPWQVTQIARHPQRPYTLDYIGDLFTEFTELHGDRSYADDLSIVGG
                     LARFNG"
BASE COUNT          434 a          863 c          829 g          379 t
ORIGIN      
        1 cacgacaccc ccgacgtttc ctggttcgca gacccgggca tcatccgatg ccacgggttc
       61 gaaagtgcca tcggctcatg acgctacgca ttcacaacac gctctcgcgc gagctcgaaa
      121 ccttcacgcc gatcgagccg aaccacgttc gcatgtacgt gtgcggcatc accatctacg
      181 acctgtgcca cgtcggccac gcgcgcatga tggtcgcgtt cgacctgatc taccgctggc
      241 tgcgcgtgct cggctaccgc gtgacctacg tgcgcaacgt caccgacatc gacgacaaga
      301 tcatccgccg cgcgctcgag cgccagattc cgatccgcca gctcaccgac gagatgatca
      361 tcgcgatgca caccgacctc gacgcgctcg gcatcgagcg cccgacccac gagccgcgcg
      421 ccaccgagta cgtgccgcag atgctcgcga tgatcggcac gctcgaggcc aaggggctgg
      481 cataccgctc gggcaacggc gacgtgaact acgcggtgcg caagttcgcc ggctacggca
      541 atctgtcggg caagtcggtc gacgaactgc gttccggcga gagagtggcg gtgctcgacg
      601 gcaaggacga cccgctcgac ttcgtgctct ggaaggcggc gaaggccgag gagcccgacg
      661 acgcgaagta cccgtccggc tacggcgcgg gccggcccgg ctggcacatc gagtgctcgg
      721 caatggcctg cgcgctgctc ggcgagcagt tcgacctgca cggcggcggc gccgacctgc
      781 agttcccgca ccacgagaac gagatcgcgc agagcgaggg cgcgaccggc cgaccgcccg
      841 cgcgcctctg ggcccacaac gggctgctga atgtcgatca ggtgaagatg tccaagtcgc
      901 tgggcaactt ctttacgatc cgcgaggtgc tggcgcgcta cgacgccgag acgatccgct
      961 tcttcatgct gcgcacgcac taccgcagcc cgttcaattt cagcgatgcg aacctcgatg
     1021 acgcgcgcgc ttcgctgcgc cgcctctaca ccgcgctcga cggcctcgac gtgccggtgc
     1081 agccaatcga ctgggccgcg ccgcaggccg cggccttccg cgccgcgatg aacgacgact
     1141 tcaacacgcc gctcgcggtg gcggtgctgt tcgaactcgc ggccgaggcc aatcgcacgc
     1201 gctcgctcga agcggccgcg ctgctgaagg gtctggccgc gacgctgggc tttctgcagc
     1261 aggtgccgcg cagctacctg cagggcggca acacgctcga cgaagcccac atcgccgagc
     1321 gcatcggggc gcgcaacgcg gccaagaagg cgggtgattt cgcacttgcc gacgcgatcc
     1381 gcgccgagct gctggcacag ggcatcgtgc tgcaggacag cgccggtggc acgacctggg
     1441 tgaaagcctg atgcccgcac gtgccgtcca gccggagctt ccggcgaccc ccgggctggc
     1501 cgccgcggcg cggatcacac cggactactg ggacgatgcc tgcaagcacc tgagccggcg
     1561 cgaccgggtg atgcgcaagc tcataccgcg gctcggcgag ggccggctgc aaagccgcgg
     1621 cgacgcgttc acgaccctcg cgcgctcgat cgtcgggcag cagatctcgg tcgcggcggc
     1681 gcaggcggtg tggacgcgct ttgcggcgct gatgagcagc ccctcgacgc ggctcgcccc
     1741 gaccgaggtg ctggcgctgg agacgtcggc cgcgcgtgcc gcgggcctgt cggcacgcaa
     1801 ggtcgagtac ctgtgcgacc tggccgcgca cttcgagtcg ggggcggtgc acgtcgacca
     1861 atggcagggc atggacgatg aggcgatcat cgacgagctg gtcgcgatcc gcggcatcgg
     1921 gcgctggacg gccgagatgt tcctgatctt ccacctgatg cgcccgaacg tgatgccgct
     1981 cgacgacctc gggctgctca agggcatcag cgtcaactat ttcagcggcg agccggtgtc
     2041 gcgtgccgag gcgcgcgagg tcggcgatgc ctgggcgccg ttccgctcgg tggccacctg
     2101 gtacatctgg cgcagcctgg atccgctgcc ggtagactac tagggttggg gtagacgcct
     2161 tcaacgcgaa ggacacaaga atgagcaaac gccatttcct cgagttcgag cagccggttt
     2221 ccgagctcga atccaagatc gaagaactgc gctacgtgca gagcgaatcg gcggtcgaca
     2281 tctcggaaga gatcgaccgc ctgggcaaga agagcctgca gctcacgaag gacatctatt
     2341 cgagcctcac gccctggcag gtgacgcaga tcgcgcgcca cccgcagcgg ccctacacgc
     2401 tggactacat cggcgacctg tttaccgagt tcaccgaact gcatggcgac cgttcgtatg
     2461 ccgatgacct gtcgatcgtc ggcggtctgg cccgattcaa cggcc
//
LOCUS       DATMJB010000078         2541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_226580, whole genome shotgun sequence.
ACCESSION   DATMJB010000078 DATMJB010000000
VERSION     DATMJB010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226580"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VNU48_01815"
     CDS             complement(<1..823)
                     /locus_tag="VNU48_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWI10044.1"
                     /translation="MAAKLPGYAELHCRSNFSFLTGASHPGELVERAQQLGYAALAIT
                     DECSLAGVVRAHVAAREAGLPLIVGSEMRLTLPTRVTTTANKNKKKEEGGGAPPPPPS
                     LPHARLVLLAQSRRGYGNLSHWITVARRRAAKGAYLAHPGDLEGKVPNAPTLAGLPEC
                     LALLVPDAAQSFETVFAHAMWLKTWFQERAWLALSLLRRPGDEELVDVARRVAQFTGL
                     RIVATGDVLMHRRSRKPLLDVLAATRLKQPVAECGWQLESNAEQHLRSRARLAALY"
     gene            complement(1000..1983)
                     /locus_tag="VNU48_01820"
                     /pseudo
     CDS             complement(1000..1983)
                     /locus_tag="VNU48_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
     gene            complement(2073..>2541)
                     /locus_tag="VNU48_01825"
     CDS             complement(2073..>2541)
                     /locus_tag="VNU48_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_045243001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HWI10045.1"
                     /translation="VLAVEMLQPKGFGRIRLRRIENDSDACVVPFVQESIEPGAQVRT
                     DGSAAYRSLSKLGYDHQRRVMLGSEVAAHVSMAGVHRVASLVKRWILGTHHGSVQPGH
                     LDAYLDEFVFRFNRRTSSSRGMLFYRLLQQAVVTEPVTYDSVVRPLHKPTQQA"
BASE COUNT          420 a          887 c          843 g          391 t
ORIGIN      
        1 cgtacagcgc ggccagccgc gcacgcgaac gcaggtgctg ctcggcgttc gactcgagct
       61 gccacccgca ctcggccacc ggctgcttca gccgcgtggc ggcgagcacg tccagcagcg
      121 gcttgcgcga gcgccggtgc atcagcacgt cgccggtggc gacgatgcgc agcccggtga
      181 actgcgcgac gcggcgcgca acgtcgacca gctcctcatc gcccggccgg cgcagcagcg
      241 agagcgcaag ccacgcgcgc tcctggaacc aggtcttcag ccacatcgcg tgcgcgaaca
      301 cggtctcgaa ggattgcgcc gcgtcgggca ccagcagcgc caggcactcg ggcaggccgg
      361 cgagcgtggg cgcgttcggc accttgcctt cgaggtcacc ggggtgcgcg aggtacgcgc
      421 ccttggcggc gcggcgccgc gccacggtga tccagtgcga caggttgccg tagccgcgcc
      481 gcgactgcgc gagcagcacc aggcgcgcat gcggcagcga tggtggcggt ggtggcgcgc
      541 cgccgccctc ttccttcttc ttgttcttgt tcgccgttgt tgtcacccgc gtcggcagcg
      601 tgagccgcat ctcgctgccg acgatcagcg gcaacccggc ctcgcgcgcc gccacgtggg
      661 cacgcaccac gccggcgagc gagcactcgt cggtgatcgc cagcgccgcg tagccgagct
      721 gctgtgcgcg ctcgaccagc tcgcccgggt gcgacgcgcc ggtcaggaag ctgaagttgc
      781 tgcggcagtg cagctcggcg taacccggta gcttggcggc catgactcgc gtacctcgtc
      841 gtgcggcggt ggatcgtcgt cgcgctcgaa tcgttctctc tcggcacggc catcgtcgtc
      901 gcgcacgaca cgccaggacg tcgacggcgc cgacgccatc gacgtggcgg ggaacagcac
      961 ccctgcggca gcgccgcccg tcagcgcccc gccgctcatc agggcatcga gcgggctcga
     1021 ggtccctgcc gcggcagatt ggagaacgtg ggtgtagacc atcgtggtgc tcacgtcgga
     1081 atggcccagc agctgctgca ccgtgcggat gtcggtgccg gcctgcagca ggtgcgtcgc
     1141 aaacgaatgg cgcagcgtgt gcaccgtcgc cggcttggcg atgccggagg cgtcgacggc
     1201 ccgcttcaga tcgcgctgca ggcgtttctc gtgcaggtga tggcggcgct tgacctggtg
     1261 ctcgtcccgg ggatcgaccg tgatgacggt cgacgggaac acccagaacc acgcccagct
     1321 ttcggccgcg cgcacgtact tggcggccag cgcgtgcggc aggtagacgc cgccgcgctc
     1381 gtggctgcgg tcgctgagcc agtactgccg cgccgattcg agctggccgc gcagggacga
     1441 ctcgagcgac cgcggcagca tcaccacccg gtccttgccg cccttgccat cgcggacgat
     1501 gatgatccgg cgaacgaaat cgacatcctt gacacgcaga cgcacgcctt cgagcaggcg
     1561 catgccggtc ccgtagagca gttgcccgag caacgccgac accccgctca tgcgcgacag
     1621 cacggcctgc acctcgccat gcgtcagcac caccggcagg cgcttcctcg gcaccggacg
     1681 gcccagctcc tgcatccacg gcatgtcgac gccgagcact tcgcggtaca ggaacagcag
     1741 ggcgctcagt gcctgccgat gggtcgaggc cgagacgtgc cgctcgttcg ccagcatgct
     1801 gaggtagccc tgcacctcgg cgacacccag cccgcgcgga tgccgcatgc cggaccatcg
     1861 gacgaagccc cgaacccaat agacataggc ctgctcggtc cgtaagctgt agtgcatgta
     1921 ccgaagccgc tcgaccaagg tctcgatcag cctgggggca cgctgcgccg agccgctgtc
     1981 catctttacc cgccccgtta atactgtgca tttataaggg gtatccactt agctccagag
     2041 aagccagaag actggatatc catccagtaa tactacgcct gctgcgttgg cttgtgcaag
     2101 ggcctgacga cgctgtcgta ggtcaccggt tcggtgacca cggcttgctg cagaaggcga
     2161 tagaagagca tgccgcgcga gctcgaagta cgccggttga agcggaacac gaactcgtcc
     2221 agataggcat ccaggtggcc cggctggacc gacccatgat gcgttcccag gatccatcgc
     2281 ttgaccagcg aggcgacccg gtgcacgccg gccatggaca catgagcggc gacctccgag
     2341 cccagcatca ccctgcgctg atggtcatag ccgagcttgc tcagtgagcg gtaggccgcc
     2401 gagccatcgg tgcgcacctg ggcgccaggc tcaatggact cttgcacgaa cggcacgaca
     2461 catgcatcgg aatcgttctc gatgcgtcgc aagcgaattc gtccaaaacc cttgggttgc
     2521 agcatttcca ccgcgaggac g
//
LOCUS       DATMJB010000079         4459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_759725, whole genome shotgun sequence.
ACCESSION   DATMJB010000079 DATMJB010000000
VERSION     DATMJB010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4459
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759725"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1519
                     /locus_tag="VNU48_01830"
     CDS             2..1519
                     /locus_tag="VNU48_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HWI10046.1"
                     /translation="MALLAGGAVQASSHREAPLLTKTPKVDGTDFYLFRSYETGRAGY
                     VTLVANYDPLQDAYGGPNYFKLDPNALYEIHVDNVGDAKEHLTFQFRFTNTSKGQTLP
                     IDGTNVAIPLTQFGGVSDTTGANLNVTESYTLSVVRGDRRSGTAAAVSGTPAGGGAAT
                     TTFAKPSDNIGTKTIPNYATYAAKFIYAINIPGCNTPGKVFVGQRKDPFAVNLGVIFD
                     LVNVPSAAFLLDNSNKDLGTDSLQDKNVTSLALEVPTSCLTAGADTVIGGWTTASLRQ
                     GMLRNAAPKSGLDNAAVHGGAWTQVSRLGMPLVNEVVIGLNDKDRFNASKPKDDAQIA
                     TYVKNPTLPKLLEIALGAPGISPTNFPRTDLVTTFLTGIKGVNQQGTVTASEMLRLNT
                     AIAPTPYANQNRLGLLGGLLSGDGDLAGYPNGRRPNDDVVDISLVAVMGGLCVANGDA
                     DTYKLGAPCKPSAVPLGTAVFGLHDGVDQAGAKVKAAGPLLPGFPYLGTPLPGAQ"
     gene            1542..1787
                     /locus_tag="VNU48_01835"
     CDS             1542..1787
                     /locus_tag="VNU48_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10047.1"
                     /translation="MKKSWILALCAAASLAGCGGGGGDHTPAVTESVPAGASASTGGF
                     VGYLMALMASAADTLEPVDMNAVKPPADDASEPTPIN"
     gene            1868..3013
                     /locus_tag="VNU48_01840"
     CDS             1868..3013
                     /locus_tag="VNU48_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10048.1"
                     /translation="MTSALAALLLGVAAGSQATPIAPTSDDEVIDVLPAVRGSRADAR
                     RLREQLAQQPQNAALALAAARRYLDQAHELGDPRFAGMAMSAIAAWPDATSAPDDVLM
                     MRASLQQYLHEFDASAATLKTLLARPGTPRPQAWLTLATVRRVQGRYAESDAACAQVG
                     RAGAVLYANACAAENAALRGDVERARATFAALLADPRQPAVTRAWLTTSVAELEQRAG
                     RPAAAETAFRTALALAPDPYTTLDYADFLIQQQRPAQALALLKGQVRSDAVLLRLAIA
                     GVQARSPGAAADVAEMRERIALANERPDAKVFHGREQAMFALAVDGDATRALVLARGN
                     VVQQREPLDLLVLAQAARASGEASALQEAKRVVDAMGLVDRRIEAAL"
     gene            3013..4245
                     /locus_tag="VNU48_01845"
     CDS             3013..4245
                     /locus_tag="VNU48_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HWI10049.1"
                     /translation="MRLAAALRHTLCGLALLVLGPTTAHAHKASDSYLQIDAGETGTT
                     LRWDIALRDLDAAIDIDTNEDARLTWAEVKTAWPRIEAYALPRLGVAGCVLRPTGHAL
                     ERRNDGAYAVLMLASDCVLAPTPAIGYTLFREVDPTHRGIAKLQRAGQPPTVVMLDPT
                     HAIGSAATTASAAPPEASAPSGALGFLREGVRHILTGYDHVLFLLCLLLPSVMRRTPE
                     PLAPGRPKAGAHPPGGWARSDRGQTHSGWRPVEKLSQAVWPVIGIVSAFTVAHSITLG
                     LAALKIVALPSALIEPAIAVTIILAALDNVWPIFPIRRVVVTFFFGLIHGFGFAGVLA
                     ELNLPTSEFVRALLEFNVGLELGQLMIVIGVTALLFVLRQWPRYRPLVIRGGSYAAIV
                     VGLLWLVERTANVSLLPM"
BASE COUNT          686 a         1584 c         1544 g          645 t
ORIGIN      
        1 cctggccttg ctcgcaggcg gcgccgtcca ggcctcgagc catcgcgaag cgcccctgct
       61 caccaagacg ccgaaagtcg acggcaccga cttctacctg ttccgcagct acgaaaccgg
      121 ccgcgccggc tacgtcacgc tggtggccaa ctacgacccg ctgcaggacg cctacggcgg
      181 cccgaactac ttcaagctcg acccgaacgc gctctacgag atccacgtcg acaacgtcgg
      241 cgacgcgaag gaacacctga ccttccagtt ccgcttcacg aacacgtcca agggccagac
      301 cttgccgatc gacggcacga acgtcgccat cccgttgacg cagttcggcg gcgtgtcgga
      361 cacgaccggc gccaatctca acgtgaccga gagctacacg ctgagcgtcg tgcgtggcga
      421 ccgccgctcc ggcacggccg cggccgtcag cggcacgccg gccggcggtg gcgccgcgac
      481 cacgaccttc gcgaagccgt ccgacaacat cgggaccaag acgattccga actacgcgac
      541 ctacgcggcc aagttcatct acgcgatcaa catcccgggt tgcaacacgc cgggcaaggt
      601 gttcgtcggc cagcgcaagg acccgttcgc cgtcaacctg ggcgtgatct tcgacctggt
      661 caacgtgccg agcgcggcct tcctgctcga caactcgaac aaggacctgg gcaccgactc
      721 gctgcaggac aagaacgtca cctcgctcgc gctcgaagtg cccaccagct gcctgaccgc
      781 cggtgccgat acggtgatcg gcggctggac gacggccagc ctgcgccagg gcatgctgcg
      841 caacgctgcg ccgaagagcg gcctcgacaa tgccgcggtg cacggcggtg cctggacgca
      901 ggtctcgcga ctcggcatgc cgctggtcaa cgaggtcgtg atcggcctga acgacaagga
      961 caggttcaac gcctccaagc cgaaggacga cgcccagatc gccacgtacg tgaagaaccc
     1021 gaccttgccc aagctgctgg agatcgcgct cggcgcaccg ggcatctcgc cgacgaactt
     1081 cccgcgtacc gacctcgtga ccaccttcct caccggcatc aagggcgtga accagcaagg
     1141 cacggtgacc gcgtcggaaa tgctgcgcct gaacaccgcg attgcgccga cgccatatgc
     1201 gaaccagaac cgcctcggcc tgctcggcgg gctgctgtcg ggcgacggcg atctggccgg
     1261 ctacccgaac ggccggcgtc cgaacgacga cgtcgtcgac atctcgctgg tcgcggtgat
     1321 gggcggcctg tgcgtggcca acggcgatgc cgacacctac aagctgggcg cgccctgcaa
     1381 gccttcggcg gtgccgctcg gcacggcggt gttcgggctg cacgacgggg tcgaccaggc
     1441 gggcgcgaag gtcaaggccg ccggcccgtt gctgccgggc ttcccgtacc tcggcacgcc
     1501 gctgccgggc gcgcagtgac gcggcgcaca aggagcatga catgaagaag tcctggatcc
     1561 ttgcgctgtg cgcggccgcg tcgctggccg gttgcggcgg cggtggaggg gaccacacgc
     1621 cagcggtcac cgagtcggtt ccggcgggtg cgagcgcgtc gacgggcggc ttcgtcggct
     1681 acctgatggc attgatggcc tcggccgccg acacgctgga gccggtcgac atgaatgccg
     1741 tcaagccccc ggccgacgac gcgagcgaac cgacgccgat caactgacgg cggcagggcg
     1801 gggcgcgcga ggcgttccgc cctcttgccg attcgattgc cgcaacgtgc aacccttcaa
     1861 gctcttgttg acatcggccc tggccgcgct gctgctcggc gtggcggcgg gctcgcaggc
     1921 gacacccatc gcgccgacca gcgacgacga ggtgatcgac gtgctgcccg cggtgcgcgg
     1981 cagccgtgcc gacgcgcgcc gcctgcgcga gcaactggcg cagcaaccgc agaacgctgc
     2041 gctcgcgctg gcggccgcgc ggcgctacct cgatcaggcg cacgaactgg gcgacccgcg
     2101 ctttgccggc atggcgatgt cggcgatcgc cgcctggccc gacgccacga gcgcgcccga
     2161 cgacgtgctg atgatgcgcg ccagcctgca gcagtacctg cacgaattcg acgcgtcggc
     2221 ggcgacgctg aagaccctgc tcgcacggcc cggcacgccg cggccgcagg cctggttgac
     2281 gctggcgacg gtgcgtcggg tgcagggccg ctatgccgaa tccgacgcag cctgcgcgca
     2341 ggtcgggcgt gccggtgccg tgctgtacgc gaacgcctgc gcggccgaga acgccgcgct
     2401 gcgcggcgac gtcgagcgtg cccgcgcgac cttcgcggcg ctgctggccg acccgcgcca
     2461 gcccgcggtg acgcgcgcgt ggctgaccac cagcgtggcc gaactggagc agcgcgccgg
     2521 tcgccccgcc gcggccgaga ccgcgttccg aacggcgctc gcgctggccc ccgatcccta
     2581 cacgaccttg gactacgcgg atttcctgat ccagcagcag cggcccgcgc aggcgctcgc
     2641 gctgctgaaa gggcaggtgc gcagcgacgc ggtgctgctg cggctggcga tcgcgggcgt
     2701 gcaggcacgc tcgccgggtg ccgcggccga cgtggccgag atgcgcgagc gcatcgcgct
     2761 ggccaacgag cggccggacg cgaaggtctt ccacggccgc gagcaggcga tgttcgctct
     2821 ggccgtcgac ggcgacgcga cccgggcgct cgtgctcgcg cgcggcaacg tcgtgcagca
     2881 gcgcgagccg ctggacctgc tggtgctcgc gcaggccgcg cgggccagcg gcgaggcgag
     2941 cgcgctgcag gaagcgaagc gcgtcgtcga cgcgatgggg ctggtcgacc gtcgtatcga
     3001 ggcggccctg tgatgcgcct ggccgccgcg ctgcggcaca cgctctgcgg cctcgcgttg
     3061 ctggtgctcg ggccgacaac agcgcacgcc cacaaggcca gcgactcgta cctgcagatc
     3121 gacgcgggcg agacgggcac gaccttgcgc tgggacatcg cgctgcgcga tctcgatgcc
     3181 gcgatcgaca tcgacacgaa cgaggacgct cggttgacct gggccgaggt gaagacggcg
     3241 tggccgcgca tcgaagccta cgcgttgccg cgcctgggcg tcgccggctg cgtgctgcga
     3301 ccgaccgggc atgcgctgga acgccgcaac gacggcgcct acgcggtgct catgctcgcc
     3361 tcggactgcg tgctggcgcc gaccccggcg atcggctaca cgctgttccg cgaagtcgac
     3421 ccgacgcacc gcggcatcgc caagctgcag cgcgcgggcc agccgccgac cgtggtcatg
     3481 ctggacccga cgcatgcgat cggctctgcc gcaacgacgg cttcggcggc accaccggag
     3541 gcgagcgcgc cgagcggcgc actcggtttc ctgcgcgagg gcgtccgcca catcctgacc
     3601 ggctacgacc acgtgctgtt cctgctgtgc ctgctgctgc cgtcggtgat gcggcgcaca
     3661 ccggagcccc tggcgccggg ccgccccaag gcaggggctc acccccctgg ggggtgggcg
     3721 cgaagcgacc gggggcaaac acactccggc tggcggccgg tcgagaagct ctcgcaggcg
     3781 gtgtggccgg tgatcggcat cgtctcggcg ttcacggtcg cgcactcgat cacgctgggg
     3841 ctggcggcgc tgaagatcgt ggcgctgccg tcggcgctca tcgagccggc gatcgcggtg
     3901 acgatcatcc tggccgcgct cgacaacgtc tggccgatct tcccgatcag gcgcgtcgtc
     3961 gtgaccttct tcttcgggct gatccacggc ttcgggtttg ccggggtgct ggcggagctg
     4021 aacctgccga cctcggagtt cgtgcgggcg ctgctggagt tcaacgtcgg gctcgaactc
     4081 gggcagctga tgatcgtcat cggcgtgacg gcgctgctgt tcgtgctgcg ccagtggccg
     4141 cgctaccggc cgctggtgat ccgtggcggc tcgtatgcgg cgatcgtcgt cgggctgctg
     4201 tggctggtcg aacgcacggc gaacgtgtcg ctactgccca tgtagaatcg ggccctcaac
     4261 gaaaaaggga cgagaaaggc aatgatggtt atgacaccgc gaactaccac cttcttccag
     4321 aaggtctagt cggccgcgcg tctggcccca ccattcccat tcccttctcg accaaggcgc
     4381 ggctcagtcc gcgccttttt gtttgctgga gcttcacgat gatctcgatt caattgcccg
     4441 acggttcgaa gcgcgaata
//
LOCUS       DATMJB010000080         5684 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_581580, whole genome shotgun sequence.
ACCESSION   DATMJB010000080 DATMJB010000000
VERSION     DATMJB010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581580"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VNU48_01850"
     CDS             complement(<1..924)
                     /locus_tag="VNU48_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWI10050.1"
                     /translation="MNAALARLPGHRFLHSPGPSRVPDEVLHAMSRQPMDLADPRVDA
                     CIAACEAGLKRLLHTEDDVFLYAANGHGAWEAVIANLVAPGAKVLIAGTGHFSESWAL
                     QTEAMGGIVIRTPWVEGLPIDPVEIEAMLRDDPLHEIAAVFAVHTDTASGITSDLAAI
                     RSAIDRAQHPALYVVDVVASLGAAPFAMDTLRCDVALGACQKGLMLPPGLAFVAVNAE
                     AMERSRSNPQPRFYWDWRLRQSPLSYRKFCGTPPQSLLAGLEAALALIEREGEARVLA
                     RHRLLADAVHAAVARWSEAGALSFFGSVPEAR"
     gene            1075..1815
                     /locus_tag="VNU48_01855"
     CDS             1075..1815
                     /locus_tag="VNU48_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004568 - chitinase activity [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HWI10051.1"
                     /translation="MLLTDIQLQQIMPKLAAAKRALYLPHLNDAMQARAIDTALRCAA
                     FVAQLAHESGEFRWMEEIWGPTTAQLRYEPPGDLAQRLGNTAPGDGRRFKGRGPIQIT
                     GRFNYGKYGELLGIDLLTDPTRAAAPEIAFATAALYWQSNGLNELADQRQFVMITRRI
                     NGGTNGLADRQMYYARVQAVLGAGAAPRGRAPALARGYEAIVADQAAPRPARVMPARR
                     KPVTKKTAPKPVPARKLAAKKAAAARRA"
     gene            2072..2557
                     /locus_tag="VNU48_01860"
     CDS             2072..2557
                     /locus_tag="VNU48_01860"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWI10052.1"
                     /translation="MSFFNPKRLSCGVVILNPDRELLLCHVTGQNHWDLPKGGIDPGE
                     TPLQAALRETREEAGLQLDAQALLDLGRLPYTDKKNLHLFALCTPRIDTKLMRCESHY
                     LDHYSGRRMPEMDGYGWFAFERIARLCTPKMALVLGNRIDLNGLLDRLTAPAPLAAAL
                     A"
     gene            complement(2564..3130)
                     /locus_tag="VNU48_01865"
     CDS             complement(2564..3130)
                     /locus_tag="VNU48_01865"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWI10053.1"
                     /translation="MDTDDPPGFTLFDTPIGPCALVWSARGLIGVALPDARAQVRRRF
                     RGAHDRAPPPDVQRAVERVLALLQGAMDDLQDIALDLRGLPAFNTRVYAIARRIPPGA
                     TRSYGEIARELGEPSAARGILPGRAARAVGQALGANPFPIIVPCHRVLAAGHRAGGFS
                     APGGARTKLRLLEIEGAPLGGTPGLFDN"
     gene            complement(3227..3580)
                     /locus_tag="VNU48_01870"
     CDS             complement(3227..3580)
                     /locus_tag="VNU48_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10054.1"
                     /translation="MATRHSAPTRPRSHHYSVYVVELDDRVWNLAGFRKANPGHVLGK
                     PCVYVGMTGLDPDLRFDRHKAGIQANRFVREYGLRLLPQLYAVYNPMPYHGACEMEVE
                     LGIALREAGYGVWQG"
     gene            3603..4796
                     /locus_tag="VNU48_01875"
     CDS             3603..4796
                     /locus_tag="VNU48_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWI10055.1"
                     /translation="MSDDPLATFIARLERALADATFARLVLAKPRTAGGDLVRVTARP
                     LTLKGAPTLSFVHTHRTRDLTKNLAFADGVQQVRALLESTFGHAHLFTASDEIQLLTS
                     KKGKRTLLQAKASHAPAPAAQHDREKHRFLDLDRAFLAELGVTDAQHRLVPLMSRKWK
                     QINKFIEVLDDALDATALKGATRLDVIDFGSGKGYLTFAMHDYLRFSLGLPARVTGVE
                     LRPDMVALCAGAAQRLALDGLHFEQGDVRTVVPEAIDIMIALHACDTATDFALHTGVR
                     AGAAIIMCSPCCHKELRPQLLSPHPLRPILKHGVHLGQQAEMLTDGLRALLLEACGYD
                     TQVFEFVSLEHTNKNKMILAVKRAASKPPDVVWQQIREIKGFYGIREQCLETLLLGDR
                     ESLVS"
     gene            complement(4834..5421)
                     /locus_tag="VNU48_01880"
     CDS             complement(4834..5421)
                     /locus_tag="VNU48_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWI10056.1"
                     /translation="MNDTYHAGMRQLQDRFDTRRLADRLDERLGRSVFTPEDRAFIES
                     RTMFFLATADAQGGPDCSYKGGDAGFVRVTGESELAFPSYDGNGMFRSLGNVLVNAAV
                     ALLFIDFEKPNRLRVNGRATINDDDDLLGAFPGAQLVVRVRAERIFPNCPRYIHRQAT
                     VEPSPYVPREGCSAPVPQWKRMEMFADVLPREGDN"
     gene            complement(5440..>5684)
                     /locus_tag="VNU48_01885"
     CDS             complement(5440..>5684)
                     /locus_tag="VNU48_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HWI10057.1"
                     /translation="ADRSKACAEYNRAQFTRCLGALEARLESREHLLGDFSLVDVAAA
                     SWLLLGNMLGLGLDSHARVAAWTRRCAERPAFKLAR"
BASE COUNT          881 a         1991 c         2002 g          810 t
ORIGIN      
        1 gcgcgcctcc ggcacgctgc cgaagaagct cagcgcgccg gcctcgctcc agcgcgcgac
       61 ggcggcatgc accgcgtcgg cgagcagccg gtggcgcgcg agcacgcgcg cctcgccctc
      121 gcgctcgatc agcgcgagcg cggcttcgag cccggcgagc agactctgcg gcggcgtgcc
      181 gcagaacttg cggtacgaca gcgggctctg gcgcagccgc cagtcccagt agaagcgcgg
      241 ctgcgggttg ctgcgcgagc gctccatcgc ctcggcgttc accgcgacga acgcgagccc
      301 gggcggcagc atcagcccct tctggcacgc gccgagcgcc acgtcgcagc gcagcgtgtc
      361 catcgcgaac ggcgccgcgc cgagcgaggc gacgacgtcg acgacgtaca gcgccgggtg
      421 ctgcgcccgg tcgatcgcgc tgcggatcgc ggcgaggtcg ctggtgatgc cgctggcggt
      481 gtcggtgtgc accgcgaaca ccgcagcgat ctcgtgcagc gggtcgtcgc gcagcatcgc
      541 ctcgatctcg accgggtcga tcggcagccc ttcgacccag ggcgtgcgga tcacgatgcc
      601 gcccatcgcc tcggtctgca acgcccacga ttccgagaaa tgcccggtgc cggcgatcaa
      661 caccttggca cccggtgcga ccagattcgc gatcaccgcc tcccaggcgc cgtggccgtt
      721 cgcggcgtac aggaacacgt cgtcctcggt gtgcagcagg cgcttcaggc cggcctcgca
      781 ggcggcgatg caggcatcga cgcgcgggtc ggccaggtcc atcggctggc ggctcatcgc
      841 gtgcagcacc tcgtcgggca cgcggctcgg gccgggcgaa tgcaggaagc ggtggccagg
      901 caggcgggcg agggcggcat tcatgcggtg gcgacggcgg gtgacggtgg gccgatgcta
      961 aacggcgcgg ccggcgcccg tcgtcagcgc ccgcgagcta gggattcagg tggccggggt
     1021 gccgtgatct gatcgcgtcg cgccggtcgg tcgccgccgg cgaaaggacg cgcgatgctc
     1081 ctcaccgata tccagctcca gcagatcatg cccaagctcg cggcggccaa acgcgcgctg
     1141 tacctgccgc acctgaacga cgcgatgcag gcgcgcgcca tcgacaccgc gctgcgctgc
     1201 gcggcgttcg tcgcgcagct ggcgcacgag tcgggcgagt tccgctggat ggaggagatc
     1261 tgggggccga ccaccgcgca gctgcgctac gagccgcccg gcgaccttgc gcagcgcctg
     1321 ggcaacaccg cacccggcga tggcaggcgc ttcaagggcc gcgggccgat ccagatcacc
     1381 gggcgcttca actacggcaa gtacggcgaa ctgctcggca tcgacctgct caccgacccg
     1441 acgcgcgcgg ccgcgcccga gatcgcgttc gcgaccgcgg cgctgtactg gcagtcgaac
     1501 ggactgaacg agctcgccga ccagcgccag ttcgtcatga tcacgcggcg catcaacggc
     1561 ggcacgaacg gcctggcgga tcggcagatg tactacgcgc gcgtccaggc ggtgctgggc
     1621 gcgggtgccg cgccacgcgg tcgcgcgccg gcgctggcgc gcggctacga ggcgatcgtc
     1681 gcggatcagg ccgcgccgcg cccggcgcgc gtgatgccgg cgcggcgcaa gcccgtcacc
     1741 aagaagaccg cgcccaagcc cgtccctgca cgcaagctcg ccgcgaagaa ggccgccgcc
     1801 gcgcgccgcg cctgagaagg tgtgaacgaa ccccatcatg cccgctcgtg ccgcgcaagc
     1861 gcacccacta gtctctgcaa acgcacgccg tagcccgagc tacggctgtg ctttgcgcct
     1921 tgattgggca cgcctgcgcg gcccgctcgg gcacgccgga ttcattcaca ccttctgagc
     1981 cggtgcgaat tacgcccgtg ttttggcggc tgttcgtcgg atggccggtg cgcgctcgtg
     2041 ccagactcct ggtgtcccat tgattctggc catgtcgttc ttcaacccca aacgtctgag
     2101 ctgcggtgtc gtgatcctga acccggatcg cgaactgctg ttgtgccacg tgacgggaca
     2161 gaaccactgg gacctcccga agggcggcat cgacccgggc gagacgccgc tgcaggcggc
     2221 gctgcgcgag acgcgcgaag aggccggcct gcagctcgac gcgcaggcgc tgctcgacct
     2281 cggccggctg ccatacacgg acaagaagaa cctgcacctg ttcgcgctgt gcacgccgcg
     2341 catcgacacg aagctgatgc gctgcgagag tcactatctc gaccactaca gcggccgccg
     2401 gatgcccgag atggacggct acggctggtt cgcgttcgag cgcatcgccc ggctgtgcac
     2461 gccgaagatg gcgctggtgc tgggcaaccg gatcgatctg aacggcctgc tcgatcgcct
     2521 gacggcgccg gcgccgctcg ccgctgcgct ggcctgaagc gcctcagttg tcgaacagcc
     2581 ccggcgtgcc gccgagcggc gcgccttcga tctcgagcag gcgcagtttg gtgcgcgcac
     2641 cgccgggcgc cgagaagccg ccggcccgat gccccgcagc aagcacgcgg tggcagggca
     2701 cgatgatcgg gaacgggttc gcgcccagcg cctggcccac ggcgcgcgcg gcgcgccccg
     2761 gcaagatccc gcgcgcagcg ctgggctcgc cgagttcgcg cgcgatctcg ccgtagctgc
     2821 gcgtcgcgcc gggcgggatg cggcgcgcga tggcgtagac gcgggtgttg aacgcgggca
     2881 ggccgcgcag gtcgagcgcg atgtcctgca ggtcgtccat cgccccttgc aacagcgcga
     2941 gcacgcgctc gaccgcgcgc tgcacgtcgg gcggcggtgc gcggtcgtgc gcgccgcgga
     3001 agcgccggcg cacctgtgcg cgcgcgtcgg gcagcgccac gccgatcagg ccgcgtgcgc
     3061 tccagaccag cgcgcagggg ccgatcggcg tgtcgaacag cgtgaagccg ggcgggtcgt
     3121 cggtgtccat cacccgcgat gatcgcaggc ctgcgccggg ccgtcccaag cggcctgcgc
     3181 tccccccggg ggacggcgcc aacggcgccc aggggctgtc aatccgtcaa ccctgccaaa
     3241 ccccatagcc cgcctcgcgc agcgcgatgc cgagctcgac ctccatctcg caggcgccgt
     3301 ggtacggcat cgggttgtag acggcgtaga gctgcggcag caggcgcagc ccgtactcgc
     3361 gcacgaagcg gttggcctgg atgccggcct tgtggcggtc gaagcgcagg tccgggtcca
     3421 ggccggtcat gccgacatag acgcaaggtt tgccgagcac atggccggga ttcgccttgc
     3481 gaaagcccgc caggttccag acccggtcgt cgagctcgac gacatagacg ctgtagtggt
     3541 gcgagcgagg gcgggtgggg gcggagtgtc gcgtggccat cggtgcctac actagcaggc
     3601 ccatgtccga cgacccgctc gccaccttca tcgcccggct cgaacgcgcg ctggccgacg
     3661 ccacgttcgc gcggctcgtg ctcgccaagc cgcgcaccgc cggcggcgac ctggtgcgcg
     3721 tgactgcgcg gccgttgacg ctgaagggcg cgccgacgtt gtccttcgtc cacacccacc
     3781 gcacccgcga cctcacgaag aacctggcgt tcgcggatgg cgtgcagcag gtgcgcgcgc
     3841 tgctggagtc cacgttcggc cacgcgcacc tgttcaccgc gagcgacgag atccagctgc
     3901 tgaccagcaa gaagggcaaa cgcacgctgc tgcaggccaa ggccagccac gcgccggcgc
     3961 cggccgcgca gcacgaccgc gagaagcacc gctttctcga cctcgaccgg gcctttctgg
     4021 ccgagctcgg cgtcaccgac gcgcagcatc ggctggtgcc tttgatgtcg cgcaagtgga
     4081 agcagatcaa caagttcatc gaggtgctgg acgacgcgct cgacgccacg gcgttgaaag
     4141 gcgcgacgcg cctggacgtg atcgacttcg gcagcggcaa gggttacctg acctttgcga
     4201 tgcacgacta cctgcgcttc agcctcggcc tgccggcgcg tgtgaccggc gtcgagctgc
     4261 ggcccgacat ggtcgcgctg tgcgcgggcg cggcgcagcg cctcgcgctc gacggcctgc
     4321 acttcgagca gggcgacgtg cgcacggtcg tgcccgaggc gatcgacatc atgatcgcgc
     4381 tgcacgcttg cgacaccgcg accgactttg ctctgcacac gggagtgcgc gccggcgcgg
     4441 cgatcatcat gtgctcgccg tgctgccaca aggagctgcg cccgcagctg ctcagcccgc
     4501 acccgctgcg gccgatcctg aagcacggcg tgcacctcgg gcagcaggcc gagatgttga
     4561 ccgacgggtt gcgcgcgctg ctgctcgaag cgtgcggcta cgacacccag gtgttcgagt
     4621 tcgtgtcgct ggagcacacc aacaagaaca agatgatcct ggcggtgaag cgcgcggcga
     4681 gcaagccgcc cgatgtggtg tggcagcaga tccgggagat caaggggttc tacgggatac
     4741 gcgagcagtg tctggagacg ctgctgctgg gtgaccgcga aagtctcgtg tcctgagatc
     4801 tggatgggtt cgcgaggcgg tcgagaaatc cggtcagttg tcgccttcgc gcggcaacac
     4861 gtcggcgaac atctccatgc gcttccactg cggcaccggc gctgagcacc cctcgcgcgg
     4921 cacgtagggc gacggctcga ctgtcgcctg gcgatgaatg tagcgcgggc agttcgggaa
     4981 gatccgctcg gcgcgcacgc ggaccacgag ttgcgccccg gggaaggcgc caagcaagtc
     5041 atcgtcgtcg ttgatggtcg cccggccatt gacgcgcaag cggttcggct tctcgaagtc
     5101 gatgaaaagc agtgccacgg ccgcgttgac gaggacgttt ccaaggctgc ggaacatgcc
     5161 gttgccatcg tagctgggga acgcgagctc gctctcgccc gtcacgcgca cgaagccggc
     5221 atctccgccc ttgtacgaac agtcagggcc gccctgtgcg tcggcggtag cgagaaaaaa
     5281 catcgtcctg ctctcgatga acgcgcgatc ctcgggggtg aacaccgagc gcccgagccg
     5341 ctcatcgagg cgatcggcaa ggcgtcgggt gtcgaagcgg tcttgcagtt gccgcatgcc
     5401 ggcgtggtag gtgtcgttca tggcagctca gtatcccgct taccgcgcca gcttgaaagc
     5461 cggacgctca gcgcagcgcc gggtccacgc ggcaacccgc gcgtgagagt cgagcccgag
     5521 ccccaacatg tttccgagca gcaaccaact cgccgccgca acatcgacga gggagaagtc
     5581 gcccaagagg tgctcgcgac tttcgagccg tgcttcgagt gcgcccaggc agcgcgtgaa
     5641 ctgcgcgcgg ttgtactcgg cgcacgcctt gctgcgatcc gcgg
//
LOCUS       DATMJB010000081         3290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_699592, whole genome shotgun sequence.
ACCESSION   DATMJB010000081 DATMJB010000000
VERSION     DATMJB010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3290
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699592"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..1305
                     /locus_tag="VNU48_01890"
     CDS             109..1305
                     /locus_tag="VNU48_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006049292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HWI10058.1"
                     /translation="MAIDVAAQIRSANAGRDPQRLQIKYRAMRASPFAFLRGTCALFY
                     ARLPRGGVFKAAPAVWACGDLHLENFGSYKADNRLTYFDINDFDESALAPASWDLVRL
                     LTSLMIGADALGVSNARARRLCQVLVEAYGAALASGRAYWVERDTATGLVQHLLDDLR
                     DRKRPAFLNSRSVVRGTRRMLRADGRKALAASDSERALVADFMRQFAKTQADPDFYAF
                     IDAARRIAGTGSLGVERYVILVQGRGSPDGNHLLDLKLALPSAIAPRLKLAQPRWKTE
                     AQRIVEVQQRAQAAPVAFLQPVFMDERAFVLRGLQPSEDRVGLDHSTAEQLEQTIATM
                     GRIVAWSQLRASGRQGSAIADELIDFGRRKKWKAELLDAAQECAAQVRQDAATYNAAW
                     DDGAFG"
     gene            1482..2525
                     /locus_tag="VNU48_01895"
     CDS             1482..2525
                     /locus_tag="VNU48_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HWI10059.1"
                     /translation="MNTAATSTYGADPSGLIYGFLIDPASGGHALESQDAAAWLGARA
                     ASGEGAPGPAGGAFLWLHFNLAHTGALPWLTRHAGLSVEFFEMLNDGLHSTRVERTEE
                     ALIAVINDVHFDFAFEPSEISTLWINVQRRLVITARRKPLRSVDKLREQVKGGQRLRS
                     SVELLEHLLRAQADVLVDIVRGVTERVDRIEDELLLNRLDHKRAGLGALRRLLVRLQR
                     LLAPEPAALFRLLQHPPAWMAPLDAQELRQSSEEFSVVLRDMSTLQERIKLLQEEIAA
                     QVNEDNNRSLFVLTIVTVLALPINIIAGLLGMNVGGVPLAQHAHGFWIVVLIVLSFTV
                     VAGWVAFRKQRER"
     gene            2661..>3290
                     /gene="modA"
                     /locus_tag="VNU48_01900"
     CDS             2661..>3290
                     /gene="modA"
                     /locus_tag="VNU48_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWI10060.1"
                     /translation="MTHATLRRGVAATLLALMSMTTQAADLTVSAAASLTNAFKELGP
                     AFEAQNPGTTVVFNFAASDALLAQIAKGAPADVFASADQQAMDRADAQKVLVAGSRRN
                     FAGNSLVMIVPGDSALGLGAPADLQRPAVKRVAIGNPASVPVGRYTKASLEAAKLWAA
                     IEPKAVLAQSVRQALDYVARGEVEAGFVYATDAAIMKDKVKVVATVPTDT"
BASE COUNT          505 a         1154 c         1133 g          498 t
ORIGIN      
        1 cgagcctgaa gaccggccgg cccaaggtcg ccgaagcttc tcgggcgccg aagggctccg
       61 gcgcggtttg accgagttcc gtgatcgtga caaacacgga gggacgcggt ggccatcgac
      121 gttgcagcgc agatcaggag cgccaatgcg ggtcgcgatc cgcaacgcct gcagatcaag
      181 taccgcgcga tgcgcgccag cccgttcgcg ttcctgcgcg gaacctgtgc gctgttctac
      241 gcccggctgc cgcgcggcgg cgtcttcaag gcggcaccgg cggtgtgggc ctgcggcgat
      301 ctgcacctcg agaacttcgg cagctacaag gccgacaacc ggctgaccta tttcgacatc
      361 aacgacttcg atgaatcggc gctggcgccg gcgagttggg atctggttcg cctgctgacg
      421 agcctgatga tcggtgccga cgcgctcggc gtgagcaacg cgcgggcacg ccgcctttgc
      481 caggtgctgg tcgaggccta cggcgcagcg ctggcgtcgg gcagggccta ttgggtcgaa
      541 cgtgacacgg ccaccgggct ggtccaacat ctgctggacg acctgcgcga ccgcaagcgg
      601 cccgcctttc tgaactcgcg cagcgtcgtg cgcggtaccc ggcgcatgct gcgcgccgac
      661 ggccggaagg cattggcggc ctccgacagc gagcgcgcgc tggtcgccga cttcatgcgc
      721 cagttcgcga aaacgcaagc cgaccccgac ttctacgcgt tcatcgatgc ggcgcgccgg
      781 atcgccggca ccggcagtct cggcgtcgag cgctatgtga tcctggtcca aggccgcgga
      841 tcgccggatg gcaaccacct gctcgatctc aagctggcgc tgccatcggc gatcgccccg
      901 cgcctcaagc tggcccagcc gcgctggaag accgaggcgc aacggatcgt cgaggtccag
      961 caacgcgcac aggccgcgcc ggtggcgttc ctccagccgg tgttcatgga tgaacgcgcc
     1021 ttcgtgctgc gcggcctgca gccgtcggaa gatcgcgtcg gcctggacca ctcgacggcg
     1081 gagcaactcg aacagacgat cgccaccatg ggccggatcg tcgcgtggtc gcagttgcgc
     1141 gcctcgggtc gccagggatc ggccatcgcc gacgagttga tcgacttcgg ccgacgcaag
     1201 aagtggaagg ccgagctgct cgacgcggcg caggaatgcg ccgcgcaagt gcgccaggac
     1261 gcggcgacct acaacgccgc ctgggacgac ggcgcgttcg gctgacaggt cgccggcgcg
     1321 gcacgccggc ggcccggccg ccctctgagc tcgagccgag agtctgcgcc ggccgccatc
     1381 cggcgcggga ttcgccaacc ggccgcgcgg gagcgtcacc ccgcgtcacg cgcctgtcac
     1441 gcccgtgtcg caaactgccc gatttggcgc gcgcatccga cgtgaacacc gcagcgacct
     1501 ccacttacgg cgccgacccc tcgggcttga tctacggctt cctgatcgac cccgcgtcgg
     1561 gcggtcacgc actcgagtcg caggacgccg cggcctggct cggcgcgcgc gccgcgagcg
     1621 gcgaaggcgc cccgggtcct gccggcggcg ccttcctgtg gctgcacttc aacctggccc
     1681 acacgggcgc gttgccgtgg ttgacgcggc acgccgggct gtcggtcgaa ttcttcgaga
     1741 tgctgaacga cggcctgcac tcgacgcgcg tcgagcgcac cgaagaggca ctgatcgccg
     1801 tcatcaacga cgtgcacttc gacttcgcgt tcgagccctc cgagatatcg accttgtgga
     1861 tcaacgtgca gcgccgcctg gtgatcacgg cgcgccgcaa gccgctgcgc tcggtggaca
     1921 agctgcgcga gcaggtcaag ggcgggcagc ggctgcggtc gtcggtcgag ttgctcgagc
     1981 atctgctgcg cgcgcaggcc gacgtcctcg tcgacatcgt gcgcggcgtg accgaacgcg
     2041 tcgatcgcat cgaggacgag ttgctgctga atcgcctcga ccacaagcgc gccggcctgg
     2101 gcgcactgcg ccggctgctg gtccggctgc agcgcctgct cgcgccggag cccgcggcgc
     2161 tgtttcggct gctccagcat ccgcccgcgt ggatggcccc gctcgatgcc caggaactgc
     2221 gccagtccag cgaggagttc tcggtggtgc tgcgcgacat gtcgacgctc caggaacgca
     2281 tcaagctgct gcaggaagag attgccgccc aggtgaacga agacaacaac cgcagcctgt
     2341 tcgtgctgac gatcgtgacc gtgctcgcgc tgccgatcaa catcatcgcc ggcctgctcg
     2401 gcatgaatgt cggtggcgtg ccgctggccc agcacgccca cgggttctgg atcgtcgtgc
     2461 tgatcgtcct gtcgttcacg gtggtcgccg gctgggtcgc ctttcgaaag cagcgggagc
     2521 gctgagcagc tcggcccagg aggcggcggc ttcacgcgcc gcgcgacggc cggcgtttcc
     2581 ccgtgccgga ttcgctatgt agtgagctat attgcgatga gccggcgcct tccgatcacc
     2641 cgcgaccctc caagacccgc atgacccacg ccacccttcg ccgcggcgtc gccgcgacat
     2701 tgctcgcgtt gatgtcgatg acgacacagg cggccgacct gaccgtctcg gcggcggcca
     2761 gcctgacgaa tgcgttcaag gaactcgggc cggccttcga agcgcagaac ccgggcacga
     2821 cggtggtgtt caacttcgcc gcctccgacg ccttgctggc gcagatcgcc aagggcgcgc
     2881 ccgccgacgt gttcgcgtcg gccgaccagc aggcgatgga ccgcgccgac gcgcagaagg
     2941 tgttggtcgc cggctcgcga cgcaacttcg ccggcaactc gctggtgatg atcgtgccgg
     3001 gcgacagtgc gctcggcctc ggggcccccg ccgacctgca aaggcccgcg gtgaaacgcg
     3061 tcgcgatcgg caacccggcc agcgtgcccg tcgggcgcta caccaaggcc tcgctcgagg
     3121 ccgcaaagct ctgggccgcc attgagccga aggcggtgct ggcgcagagc gtgcggcagg
     3181 cactcgacta cgtggcgcgc ggcgaggtcg aggccggctt cgtctacgcg accgatgcgg
     3241 cgatcatgaa ggacaaggtc aaggtcgtgg cgacggtccc gaccgacacc
//
LOCUS       DATMJB010000082         3396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_344584, whole genome shotgun sequence.
ACCESSION   DATMJB010000082 DATMJB010000000
VERSION     DATMJB010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344584"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /gene="urtD"
                     /locus_tag="VNU48_01905"
     CDS             complement(<1..846)
                     /gene="urtD"
                     /locus_tag="VNU48_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HWI10061.1"
                     /translation="MTPDLMKAGAQARIEHERRVAAAGGQSGGRAASYSRIATGELDV
                     AHGAILYLDDITVSFDGFKALNALSLSISAGEMRCIIGPNGAGKTTMMDVITGKTRPD
                     LGKAYFGSTIDLLRMRENEIASIGIGRKFQKPTVYENLSVFENLELALKADRGVRASM
                     FFRLNGEQLDRIGAMLTLIHLKDSAQRTAGLLSHGQKQWLEIGMLLMQDPKLLLLDEP
                     VAGMTDEETERTAELFLSLEGSHSLVVVEHDMKFIGELTQNGKNGKKVTVLHEGSVLA
                     EGLLSE"
     gene            complement(843..1964)
                     /gene="urtC"
                     /locus_tag="VNU48_01910"
     CDS             complement(843..1964)
                     /gene="urtC"
                     /locus_tag="VNU48_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HWI10062.1"
                     /translation="MSAVLSTPIPIATPSRGLLTGKSWSAVFGAIVVVAVLVPVLNLV
                     VPEGSAFHLSEYAVQLVGKIMCYAICALAMDLIWGYTGILSLGHGVFFALGGYCMGMY
                     LMRQIGSDGQYRLPMPDFMVFLNWKEFPWHWAVSDSFIAQMLLVVLVPGLLAFVFGYF
                     AFRSRIKGVYFSIITQAMTYAAMLLFFRNETGFGGNNGFTDFKRILGVPIATPNMRML
                     LFVITGLTLIGFFLLARWLVGSKFGRVLQAIRDAETRVMFTGYDPLRYKLAIWVISAV
                     MCGIAGALYVPQIGIINPGEMSPAASIEIAIWAAVGGRGTLIGPIVGAFFVNGAKSYF
                     TQAFPEFWLYFLGALFILVTIFLPQGIIGLVRRQKEGSA"
     gene            complement(1961..>3396)
                     /gene="urtB"
                     /locus_tag="VNU48_01915"
     CDS             complement(1961..>3396)
                     /gene="urtB"
                     /locus_tag="VNU48_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HWI10063.1"
                     /translation="DDKTVALIQALSDDAVKITDTAVFVIKDGKGFDPVSGAETPVPE
                     TAEDVVNNNEMRSELDAALASIKLFAPDDKVRAEAVKTLAGGEISEAKLPLIEKAFAQ
                     ENVPAIKDAIGRVRSAMLLGSADKAKRLDAANALGATGTPATKTLLLDRLKVETEPDV
                     KSAVEAALGQIEARLAWGERLGVIFTGISLGSILLLVALGLAITYGLMGVINMAHGEL
                     MMIGAYATYVTQNLFRSWLPGAFEWYLLLAIPASFLAAALVGAVLERSVIRWLYGRPL
                     ETLLATWGISLVLQQSVRSVFGAQNVPVENPAWLSGGVDVMSNLTLPFNRLAIIAFAV
                     LVLAGVSALIARTRLGLFVRGVTQNRQMAACMGVNTARIDTYAFALGSGLAGLAGCAL
                     SQVGNVGPDLGQGYIVDSFMVVVLGGVGQLAGTVYAAVGLGVLNKLLEGWQGAVLAKI
                     MVLVFIVVFIQKRPQGIFAMKGRSAEA"
BASE COUNT          586 a         1176 c         1095 g          539 t
ORIGIN      
        1 ctccgacagc aagccctcgg ccagcacgct gccttcgtgc agcaccgtga ccttcttgcc
       61 gttcttgccg ttctgcgtca gctcgccgat gaacttcatg tcgtgctcga cgacaacgag
      121 cgagtggctg ccttcgagcg acagaaagag ttcggcggtg cgctcggtct cttcgtcggt
      181 catgccggcc accggctcgt cgagcaacag cagcttcggg tcctgcatca gcagcatgcc
      241 gatctccagc cactgcttct ggccatggct gagcagcccg gcggtgcgct gcgcgctgtc
      301 cttcaggtgg atcaaggtca gcatcgcgcc gatgcggtcc agctgctcgc cgttcaggcg
      361 gaagaacatc gacgcgcgca cgccgcggtc ggccttcaat gcgagctcga ggttctcgaa
      421 cacgctcagg ttctcgtaga ccgtcggctt ctggaacttg cggccgatgc cgatcgaggc
      481 aatctcgttc tctctcatgc gcaagaggtc gatggtcgag ccgaagtagg ccttgcccag
      541 gtcgggccgg gtcttgccgg tgatcacgtc catcatcgtc gtcttgccgg cgccgttcgg
      601 gccgatgatg cagcgcatct cgccggcgct gatcgacagg ctcagcgcgt tcagtgcctt
      661 gaagccgtcg aagctgacgg tgatgtcgtc gaggtacagg atcgcgccgt gcgccacgtc
      721 gagctcgccg gtggcgatgc gtgaatagct cgcggcgcgg ccgcccgact ggccgcccgc
      781 cgccgcgacc ctgcgctcgt gctcgatgcg cgcctgcgcg cctgccttca tcagatccgg
      841 tgtcatgcac tgccttcctt ctggcggcgc accagaccga tgatcccctg cggcaggaaa
      901 atcgtcacga ggatgaacaa cgcgcccagg aagtagagcc agaactccgg gaaggcctgc
      961 gtgaagtagc tcttcgcgcc gttgacgaag aaggcgccga cgatcggccc gatcagcgtg
     1021 ccgcgcccgc cgaccgcggc ccagatcgcg atctcgatgc tcgccgccgg gctcatctcg
     1081 cccgggttga tgatgccgat ctgcggcacg tacagcgcac cggcgatgcc gcacatcacc
     1141 gccgagatga cccagatcgc gagcttgtag cgcagcgggt cgtagccggt gaacatcacg
     1201 cgcgtctcgg cgtcgcggat cgcctgcagc acgcggccga acttgctgcc cacgagccag
     1261 cgcgccagca ggaagaagcc gatcagcgtc aggccggtga tcacgaacag cagcatgcgc
     1321 atgttcggcg tcgcgatcgg cacgcccagg atgcgcttga agtcggtgaa gccgttgttg
     1381 ccgccgaagc cggtctcgtt gcggaagaac agcagcatcg ccgcgtaggt catcgcctgc
     1441 gtgatgatcg agaagtacac gcccttgatg cgcgagcgga acgcgaagta gccgaacacg
     1501 aacgcgagca gccccggcac cagcacgacc agcagcatct gcgcgatgaa cgaatcggac
     1561 accgcccaat gccacgggaa ttccttccag ttcaggaaca ccatgaagtc gggcatgggc
     1621 aggcggtact ggccatcgct gccgatctgg cgcatcaggt acatgcccat gcaatagccg
     1681 ccgagcgcga agaacacgcc gtggccgagc gacaggatgc cggtgtagcc ccagatcagg
     1741 tccatcgcca gcgcgcagat cgcgtagcac atgatcttgc cgaccagctg caccgcgtac
     1801 tcggacaggt ggaacgcact gccctcgggc accaccaggt tcagcaccgg cacgagcacg
     1861 gcgaccacga cgatggcacc gaacacggcg ctccagctct tgccggtgag cagaccgcgt
     1921 gacggcgtcg cgatcgggat cggcgtcgaa agaaccgcgc tcatgcttcg gcactccgcc
     1981 ccttcatcgc gaagatgcct tgcggccgct tctggatgaa cacgacgatg aagaccagca
     2041 ccatgatctt ggccagcacc gcgccctgcc agccttcgag cagcttgttc agcacgccca
     2101 ggccgaccgc tgcgtagacg gtgccggcga gctggccgac gccgccgagc acgaccacca
     2161 tgaacgagtc gacgatgtag ccctgcccca ggtcgggccc gacattgccg acctgcgaca
     2221 gcgcgcagcc cgccagcccc gccagccctg agccgagcgc gaacgcgtag gtgtcgatgc
     2281 gcgcggtgtt gacgcccatg cacgccgcca tctggcggtt ctgcgtcacg ccgcgcacga
     2341 acaggccgag ccgcgtgcgc gcgatcagcg ccgacacgcc ggccagcacc agcaccgcga
     2401 acgcgatgat cgcgaggcgg ttgaacggca gggtcaggtt gctcatcacg tcgacaccgc
     2461 ccgacagcca ggccgggttc tcgaccggca cgttctgcgc gccgaagacc gagcgcaccg
     2521 actgctgcag caccaggctg atgccccagg tcgcgagcaa ggtctcgagc gggcggccgt
     2581 agagccagcg gatcacgctg cgctcgagca ccgcaccgac cagcgccgcg gcgaggaacg
     2641 aggccggaat cgcgagcagc agataccact cgaacgcacc gggcagccag ctgcggaaca
     2701 ggttctgggt cacgtaggtc gcgtaggcgc cgatcatcat cagctcgccg tgcgccatgt
     2761 tgatgacgcc catcaggccg taggtgatcg ccaggcccag tgcgaccagc agcaggatcg
     2821 agccgaggct gatgccggtg aagatcacgc ccaggcgctc gccccaggcc aggcgcgcct
     2881 cgatctggcc cagcgccgcc tcgacggcgc tcttcacatc gggttcggtc tcgaccttca
     2941 ggcggtcgag cagcagcgtc ttggtcgccg gcgtgccggt cgcgccgagc gcgttcgcgg
     3001 catcgaggcg cttggccttg tcggcgctgc ccagcagcat cgccgagcgc acgcgcccga
     3061 tcgcgtcctt gatcgccggc acgttctcct gcgcgaaggc cttctcgatc agcggcagct
     3121 tggcttcgct gatctcgccg ccggcgagcg tcttgacggc ctcggcgcgc accttgtcgt
     3181 cgggcgcgaa cagcttgatc gatgccagcg cggcgtcgag ctcgctgcgc atctcgttgt
     3241 tgttgaccac gtcttccgca gtctcgggca ccggcgtctc ggcaccgctg acggggtcga
     3301 agcccttgcc gtccttgatc acgaaaacgg cggtgtcggt gatcttcacc gcgtcgtcgg
     3361 acagggcctg gatcagcgcc acggtcttgt cgtcgg
//
LOCUS       DATMJB010000083         2224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_758929, whole genome shotgun sequence.
ACCESSION   DATMJB010000083 DATMJB010000000
VERSION     DATMJB010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758929"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1119)
                     /gene="earP"
                     /locus_tag="VNU48_01920"
     CDS             complement(52..1119)
                     /gene="earP"
                     /locus_tag="VNU48_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HWI10064.1"
                     /translation="MGSMQWDLFCRVVDNFGDIGVCWRLAADLATRGEQVRLWVDDAS
                     ALRWMAPAGAPGVHVMRWPASDAGVDPGDVVIEAFGCELPMPFVERMARRTRAPVWIN
                     LEYLSAEAFVERAHRLPSPQQGGPGAGLTKHFFYPGFTPRTGGLIREPDLAARQRAFD
                     GSAWLAAHGAAVADGEQVVSLFCYENGALPALLDQLALRPTLLLATTGAAAHQVTALL
                     GPGLKRGALRALTLPPLSQRDYDHLLWASELNFVRGEDSFVRAQWAARPFVWQIYPQH
                     DAAHIAKLDAFLARFLAGADAPWRAGLQRFWHAWNGAGPALPALPARAPWVEHAVRWR
                     DSLRAQTDLVTQLLGFVAETR"
     gene            1171..>2224
                     /gene="uvrC"
                     /locus_tag="VNU48_01925"
     CDS             1171..>2224
                     /gene="uvrC"
                     /locus_tag="VNU48_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HWI10065.1"
                     /translation="MTDDDVPTPGDEPTDATRAQVLADVAALPNLPGVYRYFDARDAV
                     LYVGKARDLKKRVSSYFQKDHGGTRIGHMVTKIARLQTTVVRSEAEALLLENNLIKSL
                     APRYNILFRDDKSYPYLKIASHKFPRVAYYRGAVDQKHRYFGPFPSAWAVKESIQMIQ
                     KVFRLRTCEDTVFNNRTRPCLLYQIKRCSGPCVNFVSAEDYALDVNHAERFLQGEQQE
                     LMGDLQAQMMRHAQALRFEQAAEIRNQIGALSRVLHQQAVEDNTGVTTKDADILAVKV
                     EGGRACVNLAMVRGGRHLGDRPYFPKHVEDVMALGPEDDDDAAPTPASAEVQVLEAFI
                     AQHYFDGAPRAPQAAPA"
BASE COUNT          370 a          773 c          783 g          298 t
ORIGIN      
        1 cgcttcgcgc acgcggggcg tgtggccccg ttgcgcggca agcctatgat tctagcgtgt
       61 ttcggcgacg aagccgagca gctgggtcac cagatcggtt tgcgcccgga gcgagtcgcg
      121 ccaccgcacg gcgtgctcga cccacggcgc tcgcgcgggc agggccggta acgccggacc
      181 tgcgccgttc caggcgtgcc agaagcgctg cagcccggca cgccaaggcg catcggcgcc
      241 ggccagaaag cgcgccagga aggcatccag cttggcgatg tgcgccgcgt cgtgctgcgg
      301 gtagatctgc cagacgaagg gcctggcggc ccattgcgcg cgcacgaacg aatcctcgcc
      361 gcgcacgaag ttcagctcgc tggcccagag cagatggtcg tagtcgcgct gcgacagggg
      421 cggcagcgtc agcgcgcgca gggcaccgcg cttcaggccg gggccgagca acgcggtgac
      481 ctgatgcgcc gcggcgcctg tggtcgccag cagcagcgtc gggcgcagcg cgagctggtc
      541 gagcagcgcg ggcagggcgc cgttctcgta gcagaacagg ctgacgacct gttcgccgtc
      601 ggcgaccgcg gcgccgtgcg ccgccagcca cgcggaccca tcgaaggcac gctgacgtgc
      661 cgcgaggtcg ggttcgcgga tcagcccgcc ggtgcgcggc gtgaagccgg ggtagaagaa
      721 gtgcttggtc agacccgcgc cggggccgcc ctgctgcggc gagggcagcc ggtgcgcgcg
      781 ctcgacgaag gcctcggcgc tgaggtactc gaggttgatc cacaccggcg cgcgcgtgcg
      841 gcgcgccatg cgttcaacga agggcatggg cagttcgcag ccgaaggcct cgatcacgac
      901 atcgccgggg tcgacccccg catcgctcgc gggccagcgc atcacgtgca cacccggcgc
      961 gccggcgggt gccatccagc gcagcgccga cgcatcgtcg acccacagcc gcacctgttc
     1021 gccgcgcgtc gccaggtcgg ccgcgagccg ccagcacacg ccgatgtcgc cgaagttgtc
     1081 gacgacgcgg cagaacagat cccattgcat cgagcccatt attccggagt gggcgcttcg
     1141 cgcgcaggcg cagcggcaca atcgcgctcg atgaccgacg acgatgttcc cacccccggc
     1201 gacgagccga ccgacgcgac ccgcgcgcag gtgctggccg acgtcgccgc gttgccgaac
     1261 ctgcccggcg tgtaccgcta cttcgacgcg cgcgacgcgg tgctctacgt cggcaaggcg
     1321 cgcgacctga agaagcgcgt gtcgagctac ttccagaagg accacggcgg tacgcgcatc
     1381 ggccacatgg tcacgaagat cgcgcggctg cagacgacgg tggttcgctc cgaggccgag
     1441 gcgctgctgc tcgagaacaa cctgatcaag tcgctcgcac cgcgctacaa catcctgttc
     1501 cgcgacgaca agagctaccc ctacctgaag atcgcgtcgc acaagttccc tcgcgtcgcc
     1561 tactaccgcg gcgcggtgga tcagaagcac cgctatttcg gaccgttccc gagcgcctgg
     1621 gcggtgaagg aatcgatcca gatgatccag aaggtgtttc gcctgcgcac ctgcgaggac
     1681 acggtcttca acaaccgcac gcggccgtgt ctgctgtacc agatcaagcg ctgctcgggg
     1741 ccgtgcgtga acttcgtctc ggccgaagac tacgcgctcg acgtgaacca cgccgagcgc
     1801 ttcctgcaag gcgagcagca ggaactgatg ggcgatctgc aggcgcagat gatgaggcac
     1861 gcgcaggcgc tgcgcttcga gcaggccgcc gagatccgca accagatcgg tgcgctgtcg
     1921 cgcgtgctgc atcagcaggc ggtcgaggac aacaccggcg tgacgacgaa agacgccgac
     1981 atccttgcgg tgaaggtcga gggcggccgc gcctgcgtga atctcgcgat ggtgcgtggg
     2041 ggccggcacc tcggcgaccg gccgtacttc ccgaagcacg tcgaggacgt gatggcgctg
     2101 gggccggagg acgacgacga cgcggcgccg acgcccgcct cggccgaggt ccaggtgctc
     2161 gaggccttca tcgcccagca ctactttgat ggcgcgccca gggcaccgca ggcggcgccc
     2221 gccc
//
LOCUS       DATMJB010000084         2335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_591100, whole genome shotgun sequence.
ACCESSION   DATMJB010000084 DATMJB010000000
VERSION     DATMJB010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591100"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VNU48_01930"
     CDS             <1..384
                     /locus_tag="VNU48_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl dehydratase"
                     /protein_id="HWI10066.1"
                     /translation="GDALPTLDIPIGISDIVAMSIATRDFHPVHHDAQTAQRLGHPAL
                     FLNIMTTAGLVERFMRSWCGAQGWLASLKLKLGVPHYAGTTLVMSGQVTALGEDDAGR
                     WVDVAFTGSNSSGRHASGEMRIRWS"
     gene            512..1744
                     /locus_tag="VNU48_01935"
     CDS             512..1744
                     /locus_tag="VNU48_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI10067.1"
                     /translation="MNSGSVRTEMSGQTAAKTFVAVAGPSLISLVPMAVAPALPAMAQ
                     RFAQGGDGALFAQMVMAVPALMIIVAASLSGFLAERLGRRTVLLSGLLLYALAGLACL
                     IVPDAGTLIAARLVLGFAAGAVMTSSLSLVADFPEGASRDRVLGFASAGAAVMAVFAL
                     TLGGLLVEAFGWRGPFALYALALPVFVVALRAVKSVPRAPNDAENSRRRVLPMLWPVY
                     VLALVLTIGLFMPGVQGPFLLQAEGVTNAATQGMILASYSLTAAVVAACYGRIAGLLG
                     MRWQIAAAALGLGLGGIAMALLHGGALTLALGCIITGAGAGLVEPVTVSLVLQRAPAA
                     LQTRAIGLLLSAVFLGQFLNPLVVDPLRSGLGIHGAFIVVGVAFLALSALALLGGLGR
                     LANPGVAGPGATPQSTPR"
     gene            1762..>2335
                     /locus_tag="VNU48_01940"
     CDS             1762..>2335
                     /locus_tag="VNU48_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI10068.1"
                     /translation="MLSSTKAPTAGHGHDFGKACIVVAGPTLVTLIPMAAAPALPAMA
                     AKFAASFGGNGALFAQLVMTVPALMLILAAPLAGVLVERLGRRPVLLASLLLFTLAGA
                     GALVTPDAVSLMICRVLLGIAGGGVLTVCLSMAADFAEGGPRERLLGFAVAGASALAA
                     VALVGGGRLVDELGWRAPFALYLLGIPALLV"
BASE COUNT          275 a          776 c          821 g          463 t
ORIGIN      
        1 ggcgacgcgc tgcccaccct ggacattccg atcggcatct cggacatcgt cgccatgtcg
       61 atcgccacgc gcgactttca cccggtgcac cacgacgccc agaccgccca gcgcctcggg
      121 catccggcgc tgttcctcaa catcatgacc acggccggtc tggtcgagcg cttcatgcgg
      181 tcctggtgtg gggcgcaggg gtggttggcg tcgctcaagc tcaagctggg tgtcccgcac
      241 tatgcgggta cgacgctcgt catgtcgggc caggtcacgg cgcttggtga agacgatgcc
      301 ggacgctggg tggacgtcgc gttcaccggc agcaattcga gcgggcggca cgcgagcggc
      361 gagatgcgga tccgctggtc ctgactctgc ggcggccgcg ctccagcgac gctctgcgac
      421 cgttactcca aacggacgat gtgtcggcgc agcccacacc tttcaatcga gcgactaaaa
      481 acagcgccat ccggcgcgac ggagaccagc gatgaactcg ggttcggtca ggaccgaaat
      541 gtcgggacaa actgccgcga agacgttcgt cgccgtcgcc ggcccgtcat tgatctcgct
      601 tgtgccgatg gcggtcgcgc cggcactgcc cgcgatggcc cagcgctttg cgcagggcgg
      661 agacggcgcg ctgttcgcac agatggtgat ggcggtgccc gcgctcatga tcatcgtcgc
      721 ggcttcgctc tcgggtttcc tcgccgagcg gctgggccgc cgaaccgtcc tgctgtcggg
      781 acttctgctg tacgcgctgg ccggtctggc gtgtctgatc gtgccggacg caggcacgct
      841 gatcgcggcg cggctggtgc tcggctttgc cgcgggtgcg gtcatgacca gcagcctgtc
      901 gctggtggct gattttccgg aaggcgcctc gcgtgatcgc gtgctcggat tcgcgagcgc
      961 cggcgcggcc gtcatggcag tctttgcgct cacgctcggc ggtttgctgg tcgaggcctt
     1021 cggctggcgt gggccgttcg cgctgtatgc gctggcgttg ccggtttttg tggtggcgct
     1081 gcgtgccgtg aagtcggtgc cccgtgcgcc gaacgatgcc gagaacagcc ggcgccgggt
     1141 cctcccgatg ctgtggcccg tgtacgtgct cgcgctggtg ctcacgatcg ggctgttcat
     1201 gccaggcgtc cagggtccct tcctgcttca ggccgaaggc gtcacgaacg cggcaacgca
     1261 aggcatgatc ctcgcgagct actcgctgac cgccgctgtc gtcgccgcgt gttacggccg
     1321 catcgccggc ctgctcggca tgcgttggca gatcgctgcc gccgcgctcg gcctgggtct
     1381 gggcggaatc gccatggccc tgctgcacgg cggcgcgttg acgctcgcgc tgggctgcat
     1441 catcacgggc gcaggcgcgg ggctggtcga gccggtgacc gtttcgcttg tgctgcagcg
     1501 tgcacccgcg gccttgcaga cgcgagcgat cggactgttg ctgagcgcgg tctttctcgg
     1561 acagttcctc aatccgctgg tcgtcgatcc actgcgttcc ggcctcggca tccacggtgc
     1621 attcatcgtg gtcggcgtgg cttttctcgc cctgtccgca cttgccttgc tcggcggcct
     1681 cggtcgtctc gcaaacccgg gggtcgccgg tcctggcgcg accccacaat ccacgccgcg
     1741 ttaggcgagc acgaggtttg aatgttgtca tccacgaaag cgccgacggc gggccacggc
     1801 cacgattttg gcaaggcctg catcgttgtt gccggcccga ccctggtcac gctgattccc
     1861 atggcggcag cgcccgcgtt gccggccatg gcggcgaagt tcgcggcgag cttcggcggc
     1921 aacggagccc tgttcgccca actcgtgatg acggttccgg ccttgatgct cattctggcc
     1981 gcaccgctcg ccggcgtcct ggtcgaaaga ctgggccgac gcccggtgct gctggcctcg
     2041 ttgctgctgt tcacgctggc gggtgcgggt gcgctggtga cccccgacgc cgtgtcactg
     2101 atgatttgtc gcgtcctgct tggcatcgcg gggggcggcg tgttgacggt gtgcctgtcg
     2161 atggcggccg actttgccga aggtggcccg cgcgagcggc tgctcggctt tgcggtggcg
     2221 ggggcttccg ccctcgctgc ggtggccttg gtgggcggcg gtcgcctggt cgatgaactg
     2281 ggctggcgcg cgccgttcgc cctttacctc ttgggcatac ccgccctgct ggtgg
//
LOCUS       DATMJB010000085         4115 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_762483, whole genome shotgun sequence.
ACCESSION   DATMJB010000085 DATMJB010000000
VERSION     DATMJB010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762483"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VNU48_01945"
     CDS             complement(<1..351)
                     /locus_tag="VNU48_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HWI10069.1"
                     /translation="MFNKVLIANRGEIACRVAATCRRLGIRTVAVYSDADANAKHALA
                     CDEAVAIGAAAPRESYLRGERVIAAALTTGAQAVHPGYGFLSENEEFAQACADAGLVF
                     IGPPPSAIKAMGLKA"
     gene            complement(359..982)
                     /locus_tag="VNU48_01950"
     CDS             complement(359..982)
                     /locus_tag="VNU48_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HWI10070.1"
                     /translation="MHALPIDTAGLLAIAAALGWASGLRLYAVVFLTGLAGYLGWVEL
                     PAGLKLLQSPLMLGASGLMLFVEFFADKIPGVDTLWDAIHTLVRIPAGAALAAAVFGA
                     DQSSWAMAAALMGGTLAATSHLAKATTRAALNTSPEPFSNVAMSLLGDGLVPASLWLS
                     WQHPTFFFVALAVALGVMLALIVVFAKFLRRLARRVSGWFGAGQPST"
     gene            complement(989..1780)
                     /locus_tag="VNU48_01955"
     CDS             complement(989..1780)
                     /locus_tag="VNU48_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWI10071.1"
                     /translation="MTPTSTLAIVRRSAHVAEVWLNRPDVRNAFNDGVIAELHAAFTG
                     FAADTELRAVVLGGHGKAFCAGADLAWMRAMAGYDWERNRADAQALADMLWAVWCCPV
                     PVVGRVHGDCYAGGVGLAAVCDVLVAAEGVNFCLSEARLGLLPATISPYVIRAMGEQA
                     ARRYFVSAERFSAARAHAMGFVHELCTADDLDAKVAEIVAALVANGPVATRACKALVQ
                     DMAGRAIDDALRAETARRIADIRAGDEGREGVQSFLNKRKPAWLD"
     gene            complement(1827..2741)
                     /locus_tag="VNU48_01960"
     CDS             complement(1827..2741)
                     /locus_tag="VNU48_01960"
                     /inference="COORDINATES: protein motif:HMM:NF025724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HWI10072.1"
                     /translation="MMQSMNSAVRRAASWAATASLVLLTGCAATVKQDARVDGDVSRL
                     EGVTQVVALLSPDAAKQQADNPQFNRDELATRVRTRLEAKGLTAPAATHRVEIVVTDI
                     RVRGAFAAIMFGFMAGDDHVTGRVRVMDPAGRALRSFEIHASYAFGGVAGGQDGVRMN
                     WMYDKFADLAAAELEKIIVPPRAGAGSIAPASVAAPAPGPVATPAPLVAASAAAIDNA
                     EAVPVSERGRSAYREWLTRRKPRAFVVSDNGYFYAVWGTKPPDPMEPSDPTERAMKRC
                     QDAARPNCTIYAVDDRVVYTRPGPSAAR"
     gene            complement(2910..3311)
                     /locus_tag="VNU48_01965"
     CDS             complement(2910..3311)
                     /locus_tag="VNU48_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HWI10073.1"
                     /translation="MLVVDSSVWIDFFNAADDPATQMLEQLLDHGEVRLVVPDLVLFE
                     VLRGFRHERDHRQARKLMETLSIEAAGGAELALAAAQHYRNLRAQGITVRSSIDVLVA
                     TFCIEHDYALLHRDRDFDAFEELRGLRAWRH"
     gene            complement(3298..3555)
                     /locus_tag="VNU48_01970"
     CDS             complement(3298..3555)
                     /locus_tag="VNU48_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWI10074.1"
                     /translation="MRTNIVIDEKLMADAMKAGPYKTKKDAVEAGLKLLARQAAYREI
                     LKWRGKLKWEGDERIDWTAPAGEPAAAVATEPTPRKYRARR"
     gene            complement(3593..>4115)
                     /locus_tag="VNU48_01975"
     CDS             complement(3593..>4115)
                     /locus_tag="VNU48_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWI10075.1"
                     /translation="CGQRKVPLIFLQNITGFMVGRKYEAEGIAKHGAKMVTAVSTVNV
                     PKFTVIIGGSFGAGNYGMCGRAYSPRFLWMWPNARISVMGGEQAAAVLATVKRDGLEA
                     KGGAWSADEEAAFKQPILDQFEHQAHPYYASARLWDDGVIDPAQTRNVLALAISASLN
                     APIPDAKFGVFRM"
BASE COUNT          604 a         1499 c         1398 g          614 t
ORIGIN      
        1 ggccttcagc cccatcgcct tgatcgccga cggcggcggg ccgatgaaca ccagcccggc
       61 atcggcacag gcctgcgcga actcttcgtt ctcgctcagg aagccgtagc ccgggtgcac
      121 ggcctgcgcg ccggtggtca gcgccgcggc gatcacgcgc tcgccgcgca ggtagctctc
      181 gcgcggcgcc gcggcgccga tggccaccgc ctcgtcgcag gccagcgcgt gcttggcgtt
      241 cgcgtcggca tcggaataga ccgcgaccgt gcggatgccc aggcgccggc acgtcgccgc
      301 gacgcggcag gcgatctcgc ctcggtttgc aatcaagacc ttgttgaaca tgcaatcctc
      361 acgtcgacgg ttgacccgcc ccgaaccagc ccgagacgcg ccgcgcgagg cgacgcagga
      421 atttcgcgaa cacgacgatc agcgccagca tcacgcccag cgcgaccgcg agtgccacga
      481 agaagaaggt cgggtgctgc cacgacagcc acaggctcgc cggcacgagg ccgtcgccga
      541 gcagcgacat cgcgacgttc gagaacggct ccggcgaggt gttgagcgcc gcgcgcgtcg
      601 tcgccttcgc gaggtggctg gtggcggcca gcgtgccgcc catcagcgcc gcggccatcg
      661 cccagctcga ctggtccgca ccgaacaccg ccgcggcgag cgcggcgccg gcggggatgc
      721 gcaccagcgt gtggatcgcg tcccacagcg tgtcgacgcc ggggatcttg tcggcgaaga
      781 actcgacgaa cagcatcagg ccgctggccc cgagcatcag cggcgactgc agcagcttca
      841 ggccggccgg cagttcgacc cagccgaggt agccggccag cccggtcagg aacacgaccg
      901 cgtacaggcg cagcccgctc gcccagccga gcgccgccgc gatcgcgagc agcccggcgg
      961 tgtcgatcgg caacgcgtgc atcgtggatc agtcgagcca ggccggcttg cgcttgttca
     1021 gaaagctctg cacgccctcg cggccctcgt cgccggcgcg gatgtcggcg atgcggcgcg
     1081 cggtctcggc gcgcagcgcg tcgtcgatcg cgcggccggc catgtcctgc accagtgcct
     1141 tgcaggcacg cgtggccacg gggccgttcg cgaccagcgc cgcgacgatc tcggcgacct
     1201 tcgcgtccag gtcgtcggcg gtgcagagct cgtgcacgaa gcccatcgcg tgcgcccgcg
     1261 ccgcgctgaa gcgctcggcg ctgacgaagt agcgccgcgc cgcctgctcg cccatcgcgc
     1321 ggatcacata agggctgatc gtggccggca gcagcccgag ccgggcctcg ctgaggcaga
     1381 agttgacgcc ctcggccgcg accagcacgt cgcacaccgc cgccagtccg acgccgccgg
     1441 cgtagcagtc gccgtgcacg cgcccgacca ccggcaccgg gcagcaccag accgcccaca
     1501 gcatgtcggc cagggcctgt gcgtcggcgc ggttgcgctc ccagtcgtag ccggccatcg
     1561 cgcgcatcca cgccaggtcg gcgccggcgc agaatgcctt gccatggccg ccgagcacga
     1621 cggcacgcaa ctcggtgtcg gccgcgaagc cggtgaaggc cgcgtgcagt tcggcgatca
     1681 cgccgtcgtt gaaggcgttg cgcacgtcgg gccggttcag ccagacctcg gcgacgtggg
     1741 ccgagcggcg aacgatcgcc agcgtcgaag tgggtgtcat cgcaaggtct cctgaacgcc
     1801 gggaccttgc gctgcacgcg ctgcgatcag cgcgctgcgc tcgggcccgg tcgggtgtag
     1861 acgacgcggt cgtcgaccgc gtagatcgtg cagttcggac gcgccgcgtc ctggcagcgc
     1921 ttcatcgccc gctcggtcgg gtcgctgggc tccatcggat cgggcggttt cgtgccccag
     1981 acggcgtaga agtagccatt gtccgagacc acgaatgcgc gcggcttcct gcgcgtgagc
     2041 cactcgcgat aggcgctgcg accgcgctcg ctcaccggca cggcctcggc gttgtcgatc
     2101 gccgcggccg aagcggccac cagcggcgcc ggcgtcgcga ccggcccggg tgccggcgcg
     2161 gcaacgctcg ccggcgcaat cgaccccgcg cctgcgcgcg gcggcacgat gatcttctcc
     2221 agctcggccg cggccaggtc ggcgaatttg tcgtacatcc agttcatgcg aacgccgtcc
     2281 tggccgcccg cgacgccgcc gaacgcatag ctggcgtgga tctcgaagct gcgcagcgcg
     2341 cggcccgccg gatccatcac ccgcacccgg ccggtcacat ggtcgtcgcc ggccatgaac
     2401 ccgaacatga tcgccgcgaa tgcgccgcgc acgcggatgt cggtcaccac gatctcgacg
     2461 cggtgcgtcg ccgccggtgc cgtcaggccc ttggcctcga gccgcgtgcg cacgcgcgtc
     2521 gccagctcgt cgcgattgaa ctgcgggttg tcggcctgct gtttggcggc atcgggcgag
     2581 agcagcgcga cgacctgcgt cacgccctcc agacgcgaga catcgccgtc cacgcgcgcg
     2641 tcctgcttga cggtcgccgc gcatcccgtc agcagcacga gcgacgctgt cgcggcccag
     2701 ctcgccgcgc gtcgaaccgc ggaattcatc gactgcatca ttccctgctc cctggttgtc
     2761 agggagcgca tcgtaggcag cggcgcgcgc cgcgccgctg cgggcggccg cggcgcggcg
     2821 cgttcgtgtg caataggtcg cgcgtttgca cggcgcgcgc agctgctcgt gttcgggggt
     2881 gacgtacggg ctgtccatgc gacaagcgct cagtggcgcc aggcacgcag gccgcgcaac
     2941 tcttcgaacg catcgaagtc gcggtcgcgg tgcagcagtg cgtagtcgtg ttcgatgcag
     3001 aacgtggcca ccagcacgtc gatggaactg cgcaccgtga tgccctgggc ccgcaggttg
     3061 cggtagtgct gcgccgcggc cagcgccagc tcggcgcccc ccgctgcctc gatgctcagc
     3121 gtttccatca gcttgcgcgc ctggcgatgg tcgcgttcgt ggcggaaacc gcgcagcacc
     3181 tcgaacagca cgagatcggg cacgaccagc cggacctcac cgtggtcgag cagctgttcg
     3241 agcatctgcg tggccgggtc gtcggcagcg ttgaagaagt cgatccagac gctcgaatca
     3301 acgacgagca cggtatttcc tcggcgtcgg ctcggtcgcg acggccgcgg cgggctcccc
     3361 ggccggcgcg gtccagtcga tgcgctcgtc gccctcccac ttgagcttgc cgcgccactt
     3421 gagaatctcg cgatacgcag cctgccgggc gagaagcttc aacccggcct cgacggcatc
     3481 cttcttggtc ttgtacggcc cggccttcat ggcgtcggcc atgagctttt cgtcgatcac
     3541 gatgttcgta cgcatgatgt gtataaagtg attactccag gcgcatcgta catcacatcc
     3601 ggaagacccc gaacttcgca tccgggatcg gcgcgttcag gctcgccgag atcgccagcg
     3661 cgagcacgtt gcgcgtctgc gccgggtcga tcacgccgtc gtcccacagg cgcgcgctgg
     3721 cgtagtacgg gtgcgcctgg tgctcgaact ggtcgaggat cggctgcttg aacgcagctt
     3781 cctcgtccgc gctccacgcg ccgcccttcg cctccagccc atcgcgcttg acggtggcga
     3841 gcaccgccgc ggcctgctcg ccgcccatca cgctgatgcg cgcgttcggc cacatccaca
     3901 gaaagcgcgg cgagtacgcg cgcccgcaca tgccgtagtt gccggcgccg aagctgccgc
     3961 cgatgatcac ggtgaacttc ggcacattga cggtcgacac cgccgtcacc atcttggcgc
     4021 cgtgcttggc gatgccttcg gcctcgtact tgcgcccgac catgaagccg gtgatgttct
     4081 gcaaaaagat cagcggcacc ttgcgctggc cgcac
//
LOCUS       DATMJB010000086         4137 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_462294, whole genome shotgun sequence.
ACCESSION   DATMJB010000086 DATMJB010000000
VERSION     DATMJB010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462294"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2395
                     /gene="ileS"
                     /locus_tag="VNU48_01980"
     CDS             <1..2395
                     /gene="ileS"
                     /locus_tag="VNU48_01980"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HWI10076.1"
                     /translation="GNEANEIRVLKRIVERGFVYRGLKPVYWCFDCHSSLAEFEIEYA
                     DKKSQTVDVAFLAAEPARLAAAFGLPALTKDAFAVIWTTTAWTIPANQALNVGPAVEC
                     ALVDTPRGLLVLASALVEKCLARYALEGTVLATTPGARLEGLNFHHPLARVDKGYDRL
                     APVYAADYATADDGTGIVHSAPAYGIEDFNSCRAHGLAVEDILNPVQGNGQYEASLPL
                     FGGLNIWKAAPLIADALRDAGRLLATSSMVHSYPHCWRHKTPVIYRAAAQWFVRMDEA
                     GPGTAGVFATEQPAKTLRQTAIRAIEQTTFYPENGKARLHDMIANRPDWCISRQRSWG
                     VPLPFFLHKDSGELHPDTMALIDRAADIVEQGGVEAWSKLTVDDVLGAEAAQYSKSND
                     ILDVWFDSGSTFFHVLNEKVGSHPGTTGGAGADGGGPEADLYLEGHDQHRGWFHSSLL
                     IACAMEDHAPYRGLLTHGFTVDGSGRKMSKSQGNYISLQDSVQKRGAEIIRLWCAATD
                     YSGDLAIDEKILDRVVDAYRRIRNTLRFLLANTSDFDAAKHALPLAELLEIDRWALAR
                     AKQFQDEVLAHYEVYEFHPVVAKLQVFCSEDLGAFYLDVLKDRLYTTAANSAARRSAQ
                     TALWHLTHAMLRWMAPFLSFTAEEAWKVLAPGRSPSIFVETYSDTSAWGGDELLAKWA
                     RIRAIRDVANKEIEAVRGSGRLGSSLQAELTIGADAADRALLASLGDDLRFVTITSQA
                     RLVAADALTVQVEPSAATKCERCWHYRDDVGVDPAHPAICSRCVGNLFGAGETRRIA"
     gene            2398..2889
                     /gene="lspA"
                     /locus_tag="VNU48_01985"
     CDS             2398..2889
                     /gene="lspA"
                     /locus_tag="VNU48_01985"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWI10077.1"
                     /translation="MGKQASGSALLPWLGIAAIVVLLDQITKAVILRTLAVGDSHTVA
                     SFLNIVHWRNTGAAFSFLHASSGWQRWLFVGLAALAAVFIVWMLRRHGAQRLFAFALA
                     LILGGAIGNVIDRLLHGSVVDFIQLHWHEAAFPAFNVADSAITIGAGLLILDELLRVR
                     RGR"
     gene            2967..3152
                     /locus_tag="VNU48_01990"
     CDS             2967..3152
                     /locus_tag="VNU48_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HWI10078.1"
                     /translation="MRYSFDPAKQASNLKKHGLSLADARQVIESIVTAETKDHIRIIS
                     MRKADRHEQAIYRENLS"
     gene            3118..3420
                     /locus_tag="VNU48_01995"
     CDS             3118..3420
                     /locus_tag="VNU48_01995"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HWI10079.1"
                     /translation="MSKPSTAKTSADARADAPLRRADIRAGKLVLRKRGPGGAMLPNK
                     RRVNIFLDGAVIEHFKTKAGDRGYQTLINEALKQAIQAESIEGIVRKAIREELRRG"
     gene            complement(3430..>4137)
                     /locus_tag="VNU48_02000"
     CDS             complement(3430..>4137)
                     /locus_tag="VNU48_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HWI10080.1"
                     /translation="AYCTLRVMPAKTVVKLPDSISFETGAAMMLKGLTAQYLLRKTLP
                     VEGLQPGDFVLFHAAAGGVGLIACQWAKALGLQLIGTAGSDEKCALAKANGAAFTINY
                     RTEDFAARVKEITGGKGVKVVYDSVGKDTFEKSLDCLRPFGLMASFGNASGVVPPFAP
                     GILGLKGSLYLTRQTLFTHIATREATQAMADDLFAVVGSGQVKIHIDQRYPLADVAQA
                     HRDLEARKTTGCSVLLP"
BASE COUNT          691 a         1434 c         1393 g          619 t
ORIGIN      
        1 cggcaacgag gccaacgaga tccgcgtgct caagcgcatc gtcgagcgcg gcttcgtcta
       61 ccgcgggctg aagccggtgt actggtgctt cgactgccat tcgtcgctgg ccgagttcga
      121 gatcgaatac gcggacaaga agtcgcagac ggtcgacgtg gccttcctcg ccgccgagcc
      181 cgcgcggctc gccgcagcgt tcggcctgcc cgcgctcacg aaggacgcgt tcgcggtgat
      241 ctggaccacc accgcgtgga cgatcccggc gaaccaggcg ctgaacgtcg gccccgcggt
      301 cgagtgcgcg ctggtcgaca cgccgcgtgg cctgctggtg ctggccagcg cgctggtcga
      361 gaagtgtctg gcgcgctacg cgctcgaggg cacggtgctc gcgacgacgc cgggcgcgcg
      421 cctcgaaggc ctgaacttcc accacccgct ggcgcgagtc gacaagggct acgaccgcct
      481 cgcgccggtc tacgccgccg actacgcgac cgccgatgac ggcaccggca tcgtccactc
      541 ggcaccggcc tacggcatcg aggacttcaa ctcctgccgc gcgcacggcc tggcggtcga
      601 ggacatcctg aacccggtgc agggcaacgg ccagtacgaa gcgagcctgc cgctgttcgg
      661 gggcctgaac atctggaagg cggcgccgct gatcgccgat gcgctgcgcg acgctggaag
      721 gctgctcgcg acctcgtcaa tggtccacag ctacccgcac tgctggcgcc acaagacgcc
      781 ggtgatctac cgcgcggcgg cgcagtggtt cgtgcgcatg gacgaggccg gcccgggaac
      841 ggccggcgtg ttcgcgaccg agcagcccgc gaagacgctg cgccagaccg cgatcagagc
      901 gatcgagcag acgaccttct accccgagaa tggcaaggcg cgtctgcacg acatgatcgc
      961 gaaccggccc gactggtgca tcagccgcca gcgcagctgg ggcgtgccgc tgccgttctt
     1021 cctgcacaag gacagcggcg agctgcaccc cgacacgatg gcgctgatcg accgcgccgc
     1081 cgacatcgtc gaacaaggcg gtgtcgaggc ctggtcgaag ctgaccgtcg acgacgtgct
     1141 cggcgcggaa gcggcgcagt acagcaagag caacgacatc ctcgacgtct ggttcgactc
     1201 gggctcgacc ttcttccacg tgctcaacga gaaagtggga agccacccgg gcaccaccgg
     1261 cggtgctggt gccgacggcg gcggcccgga ggccgacctc tacctcgaag gccacgacca
     1321 gcatcgcggc tggttccact cgtcgctgct gatcgcttgc gcgatggaag accacgcgcc
     1381 ctatcgcggg ttgctcacgc atggcttcac ggtcgacggc tcgggccgca aaatgagcaa
     1441 gagccagggc aactacatct cgctgcagga ctcggtgcaa aagcgcggcg ccgagatcat
     1501 ccgcctgtgg tgcgcggcga ccgactactc gggcgacctc gcgatcgacg agaagatcct
     1561 cgaccgcgtc gtcgacgcct accgccgcat ccgcaacacg ctgcgcttcc tgctcgcgaa
     1621 caccagcgac ttcgatgcgg caaagcacgc gctgccgctc gccgaactgc tcgagatcga
     1681 ccgctgggcg ctggcgcgcg cgaagcagtt ccaggacgag gtgctcgcgc actacgaggt
     1741 ctacgagttc cacccggtgg tcgcgaagct gcaggtgttc tgcagcgaag acctgggcgc
     1801 gttctacctc gacgtgctga aggaccgcct gtacaccacc gcggcgaaca gcgcggcgcg
     1861 acgctcggcg cagacggcac tgtggcacct gacgcacgcg atgctgcgct ggatggcgcc
     1921 gttcctgagc ttcaccgccg aggaggcctg gaaggtgctg gcgcccggcc ggtcaccgtc
     1981 gatcttcgtc gagacctaca gcgacaccag cgcgtggggc ggcgacgagt tgctcgcgaa
     2041 atgggcacgc atccgcgcga tccgcgatgt cgcgaacaag gagatcgaag ccgtgcgtgg
     2101 cagcggccgg ctcggctcgt cgctgcaggc ggagctgacg atcggcgccg acgcggccga
     2161 ccgcgcgctg ctggcttcgc tcggcgacga cctgcgcttc gtcacgatca cgtcgcaagc
     2221 gcggctggtc gcggccgatg cgctgaccgt gcaggtcgag ccgtcggccg cgaccaagtg
     2281 cgagcgctgc tggcattacc gcgacgatgt cggcgtcgac cccgcgcacc cggcgatctg
     2341 cagccgctgc gtcggcaacc tgttcggcgc cggcgaaacc cgccgcatcg cctgagcatg
     2401 ggcaagcaag cgtccggcag cgcgctgctg ccctggctcg gcatcgccgc gatcgtggtc
     2461 ctgctcgacc agatcacgaa ggcggtgatc ctgcgcacgc tggcggtcgg tgacagccac
     2521 accgtcgcct cgttcctcaa catcgtgcac tggcgcaaca ccggcgcggc gttctcgttc
     2581 ctgcacgcgt cgtcgggctg gcagcgctgg ctgttcgtcg gcctggcggc gctggccgcc
     2641 gtgttcatcg tctggatgct gcgccgtcac ggcgcgcagc ggctcttcgc cttcgcgctc
     2701 gcgctgatcc tggggggcgc gatcggcaac gtgatcgatc ggctgctgca cggctcggtc
     2761 gtcgacttca tccagctcca ctggcacgag gccgcgttcc ccgcgttcaa cgtggccgac
     2821 agcgcgatca cgatcggcgc cggcctgctg attctcgacg aattgctgcg cgtgcggcgc
     2881 gggcggtaac cgacgcgcga tcgggcccga ggcacgccgg ggcggccatt gacattacac
     2941 atttgtatac acacaatgtg accgccatgc gctacagctt cgatcctgca aagcaggcaa
     3001 gcaacctcaa gaagcacggg ctgagcctgg ctgacgctcg ccaggtgatc gagtcgatcg
     3061 tgacggcgga gaccaaagac cacattcgca tcatttcgat gcgcaaggca gaccgacatg
     3121 agcaagccat ctaccgcgaa aacctcagct gacgcacgcg ccgacgcgcc actgcgccgt
     3181 gccgacatcc gcgccggcaa gctggtgctg cgcaagcgcg gcccgggcgg cgccatgctg
     3241 ccgaacaagc ggcgcgtcaa catcttcctc gacggtgcgg tgatcgaaca cttcaagacc
     3301 aaggccggtg accgcggtta ccagacgctg atcaacgagg ctctcaagca ggcgatccag
     3361 gccgagagca tcgaggggat cgttcgcaag gcgatccgcg aggaactgcg gcgcggttga
     3421 gtcgccggcc tacggcagca gcacgctgca accggtggtc ttgcgtgcct cgagatcgcg
     3481 gtgcgcctgg gccacgtcgg cgagcgggta gcgctggtcg atgtggatct tgacctggcc
     3541 gctgccgacc accgcgaaca ggtcgtcggc catcgcctgc gtggcctcac gcgtcgcgat
     3601 gtgggtgaac agcgtctggc gcgtcaggta gagcgacccc ttcagcccga ggatgcccgg
     3661 cgcgaacggc ggcaccacgc ccgaggcgtt gccgaagctg gccatcaggc cgaacgggcg
     3721 caggcagtcg agcgacttct cgaaggtgtc cttgccgacc gagtcataga ccaccttgac
     3781 gcccttgccg ccggtgatct ccttgacgcg cgcggcgaag tcttcggtcc ggtaattgat
     3841 cgtgaacgcc gcgccgttgg ccttggccag cgcgcacttc tcgtcggagc cggcagtgcc
     3901 gatcaactgc aggccgagcg ccttcgccca ctggcaggcg atcaggccga cgccaccggc
     3961 cgcggcatgg aacagcacga agtcgccggg ctgcagcccc tcgaccggca gcgtcttgcg
     4021 cagcaggtac tgcgcggtca ggcccttcag catcatcgcg gcgccggtct cgaacgagat
     4081 cgaatcgggc agcttgacga ccgtcttggc cggcatcacg cgcaaggtgc agtacgc
//
LOCUS       DATMJB010000087         2396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_284933, whole genome shotgun sequence.
ACCESSION   DATMJB010000087 DATMJB010000000
VERSION     DATMJB010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284933"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1138
                     /locus_tag="VNU48_02005"
     CDS             206..1138
                     /locus_tag="VNU48_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWI10081.1"
                     /translation="MNLLDAMRYLVALEQHRHFGRAASACHITQPALSNALRALEAEL
                     DVAIVRRGRSYEGLTPEGERVLASANRLLREQELLRQDLHSGIGAPRGTLIVGVVPTA
                     IPVAARFASQLQARYRGVKPVVRSLSSPEIETGLESLSLDIGLGYTERGNKFTAFAQY
                     EEQYFLLRRAAVDSGAPLRLGAVLGWREAAAMSLCLLTPEMHNRSIVDAAFRQAGVEV
                     TPAIETNSIVTAVLSVLDGEVCAVLPGALMSIVRGRTGLEALPLRAPEVRTPIGFMVA
                     AGARPSRALEAALTLAQDGAWLRHAALQGGLMTA"
     gene            1293..>2396
                     /locus_tag="VNU48_02010"
     CDS             1293..>2396
                     /locus_tag="VNU48_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWI10082.1"
                     /translation="MQQHSSSSAAATVVASISTDEMRQRIKRKSRLKGRQADDASLAD
                     VRALLGDKPAGGWARDRLIEHLHLLNDRYLGLHDRHLVALARETNVSMAEVYEVATFY
                     HHFEVLREGEEAPALTVRVCDGLSCEMAGAQDLLKRLPALLGKDVNVIAAPCVGRCEQ
                     APVAVVHQCPVPRADAAAVVAAVQGKQTRHSAAAGGSTFAPAALAERSVSERDAAATP
                     DFVDLDTYRAAGGYALAAEVVNGQRQGDDLIKALEDSGLRGLGGAGFPAGRKWRIVRE
                     QTAPRLMAVNIDEGEPGTFKDRTYLERDPHRFLEGLAVAAAVVGIDACYIYLRDEYHG
                     CRVMLQQEIARLQADPPSAGLPRIELRRGAGAYI"
BASE COUNT          344 a          836 c          874 g          342 t
ORIGIN      
        1 gcagcgaagt aaaggaggag ggacgggcgc agcccgttcc gggggacatg agcggagttg
       61 tccacccgac tctctcggct cgcgccgacc gtcaaagggc gcacgctcgc ttgcgaacga
      121 ccgcaacggg ccgaaagcag aaatccgaag ccgctgcagc gtttcatgcg tcgcgggtcg
      181 cgcaacgcgc ggtggagcaa ccgtcatgaa cctgctcgac gcgatgcgct atctggtcgc
      241 gctcgagcag caccgccact tcgggcgcgc cgcgagcgcc tgccacatca cgcagccggc
      301 gctgtcgaac gcgctgcgcg cgctcgaggc cgagctcgac gtcgcgatcg tgcgccgtgg
      361 gcgcagctac gaaggcctga cgcccgaggg tgagcgcgtg ctggcgagcg cgaaccggct
      421 gctgcgcgag caggagctgc tgcgccagga cctgcacagc ggcatcggcg cgccgcgcgg
      481 cacgctgatc gtcggcgtgg tgccgaccgc gatcccggtg gcggcgcgtt tcgcctcgca
      541 gctgcaggcg cgctaccgcg gtgtcaagcc ggtcgtgcgc tcgctgagct cgcccgagat
      601 cgagaccggt ctcgagagcc tgtcgctcga catcggcctg ggctacaccg agcgcggcaa
      661 caagttcacg gccttcgcgc agtacgagga gcagtacttt ctgctgcgcc gcgccgcggt
      721 cgacagcggt gcgccgctgc gcctcggggc cgtgctgggc tggcgcgagg ccgcggccat
      781 gtcgctgtgc ctgctgacac ccgagatgca caaccgcagc atcgtcgacg ccgcgttccg
      841 ccaggcgggc gtcgaggtga cgccggcgat cgagaccaac tccatcgtca ccgcggtgct
      901 cagcgtgctc gacggcgagg tctgcgcggt gctgccgggg gcgctgatgt cgatcgtgcg
      961 cggccgcacc ggcctggagg cgctgccctt gcgcgcgccc gaggtgcgca cgccgatcgg
     1021 cttcatggtc gcggccggcg cacggccgtc gcgcgcgctc gaggccgcgc tgacgctggc
     1081 gcaggacggc gcctggctgc gtcacgcggc actgcagggc gggctgatga ccgcgtaggc
     1141 gcgcactcgg ccgcgtgctc gcaagggttt gcccagggtc tcattcgccc ggcgaatcgt
     1201 ttggtttctc gattcaattg gacgccgcgg ggtgcgccgc ttcatagtgg cgtggtctcg
     1261 ccgcgtgggc ggcgggcgtt ccgatcgaga gcatgcaaca acactccagt tcgtcggccg
     1321 cagcaacggt ggtggcctcc atcagcaccg acgagatgcg ccagcgcatc aagcgcaaga
     1381 gccggctcaa gggccgccag gccgacgacg cgtcgctcgc cgacgtgcgt gcgttgctcg
     1441 gcgacaagcc ggccggcggc tgggcgcggg atcggctgat cgagcacctg cacctgctca
     1501 acgaccgcta cctgggcctg cacgatcggc acctggtggc gctggcgcgc gagaccaacg
     1561 tgtcgatggc cgaggtctac gaggtcgcga ccttctatca ccacttcgag gtgctgcgcg
     1621 agggcgaaga agcccctgcg ctgacggtgc gcgtgtgcga tggcctgagc tgcgagatgg
     1681 ccggcgcgca agatctcttg aagcgtctgc ctgcgctgct cggcaaggac gtgaacgtga
     1741 tcgctgcgcc ctgcgtcggc cgctgcgagc aggcgccggt ggcggtcgtg caccagtgcc
     1801 cggtgccgcg cgccgacgcg gccgcggtgg tcgcggcggt gcagggcaag cagacgcgcc
     1861 actccgctgc agccggcggc tcgacgttcg caccggcggc actcgccgaa cgcagcgtca
     1921 gcgaacgaga cgccgctgcg acgccggact tcgtcgacct cgacacctac cgcgccgccg
     1981 gtggctacgc gctcgcggcc gaggtcgtca acggccagcg ccagggcgac gatctgatca
     2041 aggcgctcga agactccggc ctgcgcggcc tcggcggcgc gggctttccg gccggccgca
     2101 agtggcgcat cgtgcgcgag cagacggcgc cgcggctgat ggcggtgaac atcgacgaag
     2161 gcgagcccgg cacgttcaag gaccgcacct acctcgaacg cgacccgcat cgttttctcg
     2221 aaggcttggc tgttgccgcg gcggtggtcg gcatcgatgc ctgctacatc tacctgcgcg
     2281 acgagtacca cggctgccgc gtcatgctgc agcaggaaat cgcacgcctg caggccgacc
     2341 cgccgtcggc ggggctgccg cgcatcgagc tgcggcgcgg cgccggcgcc tacatc
//
LOCUS       DATMJB010000088         2538 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_764023, whole genome shotgun sequence.
ACCESSION   DATMJB010000088 DATMJB010000000
VERSION     DATMJB010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764023"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VNU48_02015"
     CDS             <1..705
                     /locus_tag="VNU48_02015"
                     /inference="COORDINATES: protein motif:HMM:NF016252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HWI10083.1"
                     /translation="DSDGLSVATGNADWLWRPLVNPKRLLVTSFAAAQLQGFGLMQRD
                     RSSASYEDPEAQYERRPSAWIDPTGNWGPGRVELVQIPTPDETNDNIVAYWVPDKPPD
                     PKKPFDFAYQIRWQMSGQLPLGKAWVVQTRRGRGYVKQPDGDLNFIVDFDGPPLRELK
                     SGAKAEAFVELGGNAQLREKNLFLNPVTGVWRMTVRVRREDAAKPIEMRGQIREGGRV
                     LTETWSYIVPPESDKP"
     gene            702..>2538
                     /gene="mdoH"
                     /locus_tag="VNU48_02020"
     CDS             702..>2538
                     /gene="mdoH"
                     /locus_tag="VNU48_02020"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HWI10084.1"
                     /translation="MSTPGRPKGEYRSAPREGTPVTAPIGDIDARIEPMPRIHRRSMA
                     QQRWAGHPLWRPVRRLLKGRAPQTWESANPDRHLMLRRVLLLVLVTLGAIIGTDYMID
                     IMPQHGRDWAERGLLTLFAILFAWVSAGFWTAVLGAWVMLRGHDRHAVTNVLRGAGGA
                     ATIDPEARTAVIMPICNEDVPTVFAGLRATCESLARTGFGERFDFFVLSDTNDPDARA
                     AEQVAWAQLTDALRLAGEPIRVHYRWRVHRTHRKAGNVADFCRRFGADYRYMIVLDAD
                     SVMSGDCLASLVRMMEAHPDTGIIQTAPRAAGHETIHARILQFCSRVYGPLFTTGLHY
                     WQLGESHYWGHNAILRLAPFIAHCALPPLPGRTSLSGGVMSHDFVEAALMRRAGWKVW
                     IAHDLEGSYEQVPPNLLAELGRDRRWAHGNLQNSRLVFEPGLHPVHRTVFLTGVLAYL
                     SSPFWLAFLLLSTVLFATHSHLVPTYFVEPYQLFPIWPTANFKLMLTLFGLTAVLLLA
                     PKVISLAAIVVRGQAACYGGVAKLLLSALLEFLHSVLLAPVRMLFHTQFVLVALTGLK
                     LDWKSPPRDDTATGWGEALRRHGLHTLLALAWIGAILISAAAFQWW"
BASE COUNT          371 a          890 c          876 g          401 t
ORIGIN      
        1 gattccgatg gcctgtcggt cgccaccggc aacgccgact ggctgtggcg cccgctggtg
       61 aacccgaagc gcctgctcgt gacctcgttc gccgcggctc agctgcaggg cttcggcctg
      121 atgcagcgcg accgctcgtc ggccagctac gaagacccgg aggctcagta cgagcgccgg
      181 ccgagtgcct ggatcgaccc gacgggcaac tgggggccgg ggcgggtcga gctggtgcag
      241 atccccacgc cagacgagac caacgacaac atcgtcgcgt actgggtgcc cgacaagccg
      301 cccgacccga agaagccctt cgacttcgcc taccagatcc gctggcagat gagcggccag
      361 ctgccgctgg gcaaggcctg ggtcgtgcag acgcggcgcg gccgcggcta cgtgaagcag
      421 cccgacggcg acctgaactt catcgtcgac ttcgacggcc cgccgctgcg cgaactcaag
      481 tccggcgcca aggcagaggc cttcgtcgaa ctcggcggca acgcgcagct gcgcgagaag
      541 aacctgttcc tgaacccggt caccggcgtc tggcgcatga cggtgcgcgt caggcgcgag
      601 gatgccgcca agccgatcga gatgcggggt cagatccgcg agggcggtcg tgtgctgacc
      661 gagacctgga gctacatcgt gccgccggag tcggacaagc catgagcacg ccgggccgcc
      721 ccaagggcga ataccggagc gcaccgcgcg aaggtactcc agtgaccgcg ccgatcggtg
      781 acatcgacgc tcgcatcgaa ccgatgccgc gcatccatcg ccgctcgatg gcgcagcagc
      841 gctgggcagg gcacccgctg tggcggccgg tgcgccggct gctgaagggc agggcgcccc
      901 agacctggga gagcgcgaat ccggaccgtc acctgatgct gcgccgcgtg ctgctgctgg
      961 tgctggtcac gctcggcgcc atcatcggca ccgactacat gatcgacatc atgccgcagc
     1021 atggtcgcga ctgggccgag cgcgggctgc tcacgctgtt cgcgatcctg ttcgcctggg
     1081 tgtcggccgg gttctggacc gcggtgctcg gtgcatgggt gatgctgcgc gggcacgacc
     1141 ggcacgcggt gacgaacgtg ctgcgcggcg cgggcggcgc cgcgacgatc gaccccgagg
     1201 cgcgcactgc ggtgatcatg ccgatctgca acgaggacgt tccgacggtg ttcgccggcc
     1261 tgcgcgcaac ctgcgaatcg ctggcgcgca ccggcttcgg cgaacgcttc gacttcttcg
     1321 tgctcagcga caccaacgat ccggatgccc gcgccgccga gcaggtcgca tgggcgcagc
     1381 tgaccgacgc gctgcgcctg gccggcgaac cgattcgcgt gcactaccgc tggcgcgtgc
     1441 accgcaccca ccgcaaggcc ggcaacgtgg ccgacttctg ccggcgcttc ggtgccgact
     1501 accgctacat gatcgtgctc gatgccgaca gcgtgatgag cggcgactgc ctggcctcgc
     1561 tggtgcgcat gatggaggcg catcccgaca ccggcatcat ccagacggcg ccgcgcgcgg
     1621 ccggccacga gacgatccac gcgcgcatcc tgcagttctg ctcgcgcgtg tacggcccgc
     1681 tgttcacgac cgggctgcac tactggcagc tcggcgaatc gcactactgg gggcacaacg
     1741 cgatcctgcg cctggcgccg ttcatcgcgc actgcgcact gccgccgctg ccgggcagga
     1801 cctcgttgtc gggcggcgtg atgtcgcacg atttcgtcga ggccgcgctg atgcgacgcg
     1861 ccggctggaa ggtctggatc gcgcacgatc tcgaaggcag ctacgagcag gtgccgccga
     1921 acctgctggc cgaactcggg cgcgaccggc gctgggccca cggcaacctg cagaactcgc
     1981 ggctggtgtt cgagccgggc ctgcacccgg tgcatcgcac ggtgttcctg accggcgtgc
     2041 tggcctacct ttcgtcgccg ttctggctcg cgttcctgct gctgtcgacg gtgctgttcg
     2101 caacccattc gcacctggtg ccgacctact tcgtcgagcc ctatcagctg ttcccgatct
     2161 ggccgaccgc gaacttcaag ctgatgctga cgctgttcgg cctgaccgcg gtgctgctgc
     2221 ttgcgccgaa ggtgatctcg ctcgcggcca tcgtcgttcg cgggcaggcg gcgtgctacg
     2281 gcggcgtggc gaagctgctg ctcagcgcgc tgctcgagtt cctgcactcg gtgctgctcg
     2341 cgccggtgcg catgctgttc cacacccagt tcgtgctggt cgcgctgacc gggctgaagc
     2401 tggactggaa gtcgccgccg cgcgacgaca ccgccaccgg ctggggcgag gcactgcgcc
     2461 ggcatggcct gcacacgctg ctcgcgctgg cctggatcgg cgcgatcctg atcagcgccg
     2521 ccgccttcca gtggtggt
//
LOCUS       DATMJB010000089         2289 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_580486, whole genome shotgun sequence.
ACCESSION   DATMJB010000089 DATMJB010000000
VERSION     DATMJB010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580486"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VNU48_02025"
     CDS             <1..301
                     /locus_tag="VNU48_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWI10085.1"
                     /translation="GLRLRPNKVLVDGNQLPVLKVAAEAIVQGDAKIKAISAASILAK
                     VYRDRLCQELHLQHPQYGFDDHKGYATPQHLAALKAHGACPHHRRSFAPVRAAVR"
     gene            298..1074
                     /locus_tag="VNU48_02030"
     CDS             298..1074
                     /locus_tag="VNU48_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWI10086.1"
                     /translation="MSSSIKAISSRDNSLLVRLRKLAADPAAYRKLGEVWVEGEHLCS
                     AFAQRGGVAAQAVISEAGWQVPALRALAGTAAAVAVVPEALMPGLSALESPPPLAFAL
                     PWAGPGELDATTPSVVLDRLQDAGNVGNILRSAAAFGFPQVIALKGTAALWSPKVLRA
                     GMGAHFGLRLVEGAEVDALAALELPLLATSSHATLAIHESSLPWPCAWVLGHEGQGVA
                     AALLRRCAQTLRIAQPGGEESLNVAAAAAVCLHESVRQRG"
     gene            complement(1338..2174)
                     /locus_tag="VNU48_02035"
     CDS             complement(1338..2174)
                     /locus_tag="VNU48_02035"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HWI10087.1"
                     /translation="MAQRTVFFVSDGTGITAETFGNSIMSQFAVRSRHVRRPFIDTAD
                     KAHQVLREINHTADSEGQKPIVFITLVNPEILAILKGGSAGVEQKGLVLDMFNTFIEP
                     LEAEFAMTSNHRVGRFADVSKSQEYTDRIEAINFSLAHDDGQSAKNLASADVILVGVS
                     RSGKTPTSLYLAMQHGIKAANYPLIPEDFDRGNIPSTLAPFKKKCFGLTIDPERLSHI
                     RNERRPGSKYAAVENCRYEVREAETMMRREGIAWLSSTHKSIEEIATTILRDLRPDRL
                     MY"
BASE COUNT          324 a          748 c          835 g          382 t
ORIGIN      
        1 ggggctgcga ctgcgaccga acaaggtgct ggtcgatggc aaccagctgc cggtgttgaa
       61 ggtcgcggcc gaagcgattg tccaaggcga tgccaagatc aaggcgatct cggcggcgtc
      121 gattctcgcg aaggtctacc gcgatcggct gtgccaggaa ctgcacctgc agcatccgca
      181 gtatggcttc gacgaccaca agggttacgc gacgccgcaa catcttgccg cgctgaaggc
      241 gcacggggca tgcccgcatc accggcgcag cttcgcaccg gtgcgcgctg ctgtgagatg
      301 agctcatcga tcaaggcgat cagctcgcgc gacaactcgc tgctggtgcg gctgcgcaag
      361 ctcgcggccg acccggccgc gtatcgcaag ctgggcgagg tctgggtcga gggcgaacac
      421 ctgtgctcgg cgttcgcgca gcgcggcggc gtggcggcgc aggcggtgat cagcgaggcg
      481 ggctggcagg ttccggcgct gcgcgcgctg gccggtaccg cggcggccgt cgcggtcgtg
      541 cccgaggctc tgatgcccgg cctgagcgcg ctcgagtcgc cgccgccgct ggcgttcgcg
      601 ctgccgtggg cgggcccggg cgagctcgat gcgacgacgc ccagcgtcgt gctcgaccgg
      661 ctgcaggacg ccggcaacgt cggcaacatc ctgcgcagtg cggcggcctt cggcttccct
      721 caggtgatcg ccctcaaggg cacggccgcg ctgtggtcgc cgaaggtatt gcgcgccggc
      781 atgggcgcgc atttcggcct gcggctggtc gagggcgccg aggtcgatgc gctggcggcg
      841 ctcgagttgc cgttgctggc gacgagctcg cacgcgacgc tggcgatcca cgagtcatcg
      901 ctgccatggc cctgcgcgtg ggtgctgggc cacgaagggc agggcgtggc ggcggcgctg
      961 ctgcggcgtt gcgcgcagac gctgcgcatt gcgcagccgg gcggggagga atcgctgaac
     1021 gtggcggcgg ccgccgccgt gtgcttgcac gaatccgtgc ggcaacgcgg ctagggcctg
     1081 ttaacgctat gggagggctc gcgtgactca gcaaacagcc gccagcaagg cgcacggcgc
     1141 agcccaggct gtacgcctgg gcaagccgtg caacgcggct ggcggctgtt tgctgagatc
     1201 acccgaaggg ccggcgcctg gcaggcccca gggcgtcgtt gctcactcgt tgcgtgccaa
     1261 ggcacgccgc ctcgctcgcg cctagccctg gggcctgcca ggcgccggcg cgagccctcc
     1321 catagcgtta acaggcccta gtacatcaag cgatccggcc gcaggtcgcg cagaatcgtc
     1381 gtcgcgatct cctcgatcga cttgtgcgtc gacgacagcc atgcgatgcc ctcgcgccgc
     1441 atcatcgtct cggcctcgcg gacctcgtag cggcagttct cgaccgcggc gtacttgctg
     1501 cccggccggc gctcgttgcg gatgtggctc aggcgttcgg ggtcgatcgt caggccgaag
     1561 cacttcttct tgaagggcgc cagcgtcgac gggatgttgc cgcggtcgaa gtcttccggg
     1621 atcagcgggt agttcgccgc cttgatgccg tgctgcatcg ccaggtacag cgaggtcggc
     1681 gtcttgccgc tgcggctgac gccgaccagg atcacgtcgg cgctggcgag gttcttcgcc
     1741 gactggccgt cgtcgtgggc aagcgagaag ttgatggcct cgatgcggtc ggtgtactcc
     1801 tggctcttcg acacatcggc gaagcgcccg acgcggtggt tcgaggtcat ggcgaactcg
     1861 gcctccagcg gctcgatgaa ggtgttgaac atatcgagca ccaggccctt ctgttcgacg
     1921 ccggcgctgc cgcccttcag gatcgcgagg atctccgggt tcaccagcgt gatgaagacg
     1981 atcggcttct gcccctcgct gtcggcggtg tggttgatct cgcgcagcac ctggtgcgcc
     2041 ttgtcggcgg tgtcgatgaa ggggcggcgc acgtggcgcg accgcaccgc gaactgcgac
     2101 atgatcgagt tgccgaaggt ctcggcggtg atgccggtgc cgtcggagac gaagaacacg
     2161 gtgcgttggg ccatggcggg cgcgcggggc gcgaagtgga gcttgccggc gatgataggc
     2221 agttcgcccg taaaatcgcc gcaatctcgc ccacgctccc tgccgatgaa cgcccccgcc
     2281 tgctgcgcc
//
LOCUS       DATMJB010000090         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_343655, whole genome shotgun sequence.
ACCESSION   DATMJB010000090 DATMJB010000000
VERSION     DATMJB010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343655"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VNU48_02040"
     CDS             <1..1145
                     /locus_tag="VNU48_02040"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWI10088.1"
                     /translation="SVEVQPSATAAPGATARILREPKGQVLIFGPWNFPFALLLQPLV
                     GAIAAGNVCVLKPSEFTPATSAVCARLLRATFDEAEVAVVEGGPEIATALLERPFDHI
                     FFTGSTQVGKLVMAAAARHLSSVTLELGGKSPVIIDDDVDLAQAAARIAWGKFMNAGQ
                     ICLSPDYILVRAAQRDAFVAEVARCVGASLHTDGALVEADLSQIVNERNLRRLAALIA
                     DAVGRGARIALGGKVLASRRIEPTILVDVPRDAAVMQEEIFGPIMPVLTYETLDDALN
                     FVNGRDKPLALYVFSRRTAFVDAVLARTSSGGVTVNDVIAHVAEPNLPFGGVGPSGLG
                     SYHGYYSFLAFSHERSVYGQAAENPMEAFARPPYAGKLEMLTGMIS"
     gene            complement(1152..>2079)
                     /locus_tag="VNU48_02045"
     CDS             complement(1152..>2079)
                     /locus_tag="VNU48_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWI10089.1"
                     /translation="FLNACTHRAGRLKDGSGKTASLSCPYHAWGFDLKGQLRTVYEEK
                     TFGTIDKSFYNLVRLPVEEKYGMVFAGLAPDVRVSIDEILGEMAPLFASWNLETAKVV
                     DSHEWHMKTNWKLALDTFCEGYHFTPLHKATLGDVSHGNLSLVDYFGPEQRNHRVAFP
                     NTSISKLRDKPESSWGLEIFHEFQLVHFLYPNISLLVSPSAVELFQLYPGKTVDEHMT
                     RYRCYWRTQEGGAGWGVDDPQAHFNFVRDIVTKEDYWVSANVMKSLKGGLRSFNTFGR
                     NEPALHNMHRAFARGVGRALEQAPLVMDGAAR"
BASE COUNT          312 a          709 c          688 g          370 t
ORIGIN      
        1 agtcggtcga ggtgcaaccc agcgccaccg cggcgccggg tgccacggcg cgcatcctgc
       61 gcgagcccaa gggccaggtg ctgatcttcg ggccttggaa ctttcccttt gcgctgttgc
      121 tgcagccgct ggtcggcgcc atcgccgcgg gcaatgtgtg cgtgctgaag ccgtccgaat
      181 tcacgcccgc gaccagtgcc gtctgcgcac gcctgctgcg cgccaccttc gacgaggcgg
      241 aagtcgcggt cgtcgaaggc ggccccgaga tcgccacggc gctgctcgaa cggcccttcg
      301 accacatctt cttcaccggt tccacccagg tgggcaagct ggtgatggcc gcggcggccc
      361 gccacctgtc gtcggtgacg ctggagctcg gcggcaagtc gcccgtcatc atcgatgacg
      421 acgtcgacct ggcgcaagcg gccgcgcgca tcgcctgggg caagttcatg aacgcggggc
      481 agatctgcct gtcgcccgac tacatcctgg tgcgggccgc gcagcgcgat gccttcgtgg
      541 ccgaggtcgc ccgctgcgtg ggtgcgtcgc tgcacaccga tggcgccttg gttgaggcgg
      601 acctgagcca gatcgtcaac gagcgcaacc tgcgccggct cgccgcactg atagccgacg
      661 cggtgggccg cggtgcccgc atcgcgctgg gcggcaaggt gctggcctcg cggcgcatcg
      721 agccgaccat cctggtcgat gtgccgcgcg acgccgccgt catgcaggaa gagatcttcg
      781 gccccatcat gccggtgctc acctatgaga ctctcgacga cgcgctgaac ttcgtcaacg
      841 ggcgcgacaa gccgctggcg ctgtacgtgt tcagccgccg cacggcgttc gtcgacgcag
      901 tgctggcgcg cacgtcgtcg ggaggcgtca ccgtcaacga cgtgattgcc catgtcgcgg
      961 agcccaatct gcccttcggc ggcgtggggc cgagtggcct gggttcgtac cacggctact
     1021 actcgttcct cgccttcagc catgagcgca gcgtctatgg ccaggcggcc gagaacccga
     1081 tggaggcctt tgctcgcccg ccctatgcgg gcaagctcga gatgctgacc ggcatgatca
     1141 gctgacccgc gtcacctcgc cgcgccatcc atcaccaggg gtgcctgttc cagcgcacgg
     1201 cccacgccgc gcgcgaaggc gcggtgcatg ttgtgcagcg ccggctcgtt gcgcccgaag
     1261 gtgttgaacg aacgcagccc gcctttcagg ctcttcatca cgttggcgct gacccagtag
     1321 tcttccttgg tgacgatgtc gcgcacgaag ttgaaatggg cctgcgggtc gtcgacgccc
     1381 cagccggccc cgccttcctg cgtgcgccag tagcagcggt agcgcgtcat gtgctcgtcc
     1441 accgtcttgc ccgggtacaa ctggaacaac tccaccgcgc tcggcgacac gagcagcgag
     1501 atgttcgggt acaggaagtg cacgagctgg aactcgtgga agatctccag cccccagctt
     1561 gattccggct tgtcgcgcag cttcgagatg ctggtgttcg ggaacgccac ccggtggttg
     1621 cgctgctcgg ggccgaagta gtcgaccaac gacaggttgc cgtgcgagac gtcgcccagc
     1681 gtcgccttgt gcagcggcgt gaagtggtag ccctcgcaga aggtgtcgag cgcgagcttc
     1741 cagttggtct tcatgtgcca ttcgtggctg tcgaccacct tggcggtctc caggttccag
     1801 ctcgcgaaca gcggcgccat ctcgcccagg atctcgtcga tcgacacgcg cacgtcgggt
     1861 gccaggccgg cgaagaccat gccgtacttc tcctcgaccg gcaggcgcac gaggttgtag
     1921 aacgacttgt cgatcgtgcc aaaggtcttt tcctcgtaca ccgtgcgcag ctggcccttc
     1981 aggtcgaagc cccaggcgtg gtacgggcag ctcagcgacg ccgtcttgcc gctgccatct
     2041 ttcagccgcc cggcgcggtg cgtgcaggca ttgaggaag
//
LOCUS       DATMJB010000091         2412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_343596, whole genome shotgun sequence.
ACCESSION   DATMJB010000091 DATMJB010000000
VERSION     DATMJB010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343596"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /gene="murB"
                     /locus_tag="VNU48_02050"
     CDS             complement(<1..953)
                     /gene="murB"
                     /locus_tag="VNU48_02050"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HWI10090.1"
                     /translation="MQIERGVSLKPYNSFGLPAVAGTLVRVASDADVRRVVDHPQLGT
                     APKFVLGGGSNIVLTHDMPQLVVKVEVRGIRLVEERADAWVVEAGAGEPWHAFVAHTL
                     AQGWPGLENLALIPGTVGAAPVQNIGAYGVELKDRFESLDVVDLLTGRTSTLRAERCA
                     FGYRDSVFKHALAGRTVITRVRFRLPRPWQPVLGYLDLERKIAETGIRAPSAQQVADW
                     VCAIRRAKLPDPVLLGNAGSFFKNPLVTPEQCRDIIGRDPEIVHYPLPDGSIKLAAGW
                     MIDACGWKGKSVGQAGVYERQALVLVNRGGAIGSEVMLLAGA"
     gene            1120..1608
                     /locus_tag="VNU48_02055"
     CDS             1120..1608
                     /locus_tag="VNU48_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HWI10091.1"
                     /translation="MPSFDAVLEPNLVEVRNAVDQTAKEIGTRFDFKGSSARVELKEK
                     ELTVWADSDFQIGQVLDVLIAKLTKRGVDIRFLDRSAKIEKIGGDKVRQPLTVKSGID
                     SDTAKKIQNFVKQSKLKVQAAIQGDTVRVTGAKRDDLQAAMALIRKEIADAPLSFTNF
                     RD"
     gene            1612..2247
                     /locus_tag="VNU48_02060"
     CDS             1612..2247
                     /locus_tag="VNU48_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HWI10092.1"
                     /translation="MRGVAAVALLLLAAPAFAQSVSLSGSLGSKALLVIDGTPRSVAV
                     GTSVQGVKLVAVSGSDAVVEIKGKRVTLLMGGAQVNLGGAASEGTGSQIVLSADSGGH
                     FFSSGAINGKPAHFLVDTGATFVSMGAAQAQQLGIDFARGQRGISDTANGPMVVYKVK
                     LASVRVGDVLLYDVDGLVSQQPMGAVLLGNSFLARFQMKRENDTMTLTRRY"
BASE COUNT          402 a          831 c          834 g          345 t
ORIGIN      
        1 gcgcccgcga gcagcatcac ctcggaaccg atcgcaccgc cgcggttcac cagcaccagc
       61 gcctgccgct cgtagacgcc ggcctggccg acgctcttgc ccttccagcc gcaggcgtcg
      121 atcatccagc cggccgcgag cttgatgctg ccgtcgggca gcgggtagtg cacgatctcg
      181 gggtcgcggc cgatgatgtc gcggcactgc tcgggcgtca cgagcgggtt cttgaagaag
      241 ctgccggcgt tgccgagcag caccgggtcg ggcagcttgg cgcggcggat cgcgcagacc
      301 cagtcggcca cctgctgcgc gctcggcgcg cggatgccgg tctcggcgat cttgcgttcg
      361 aggtcgaggt agccgagcac cggctgccac ggcctgggca ggcgaaaacg cacgcgcgtg
      421 atcacggtgc ggcccgccag cgcatgcttg aacacgctgt cgcggtagcc gaaggcgcag
      481 cgttcggcgc gcagggtcga cgtgcggccg gtcagcagat cgacgacgtc gagcgactcg
      541 aagcggtcct tcagctcgac cccgtaggcg ccgatgttct gcaccggcgc ggcgccgacc
      601 gtgccgggaa tcagcgcgag gttctccagc cccggccagc cttgcgccag cgtgtgcgcg
      661 acgaacgcgt gccagggctc gccggcgccg gcctcgacga cccaggcatc ggcgcgctcc
      721 tcgaccaggc ggatgccgcg cacctcgacc ttgacgacga gttgcggcat gtcgtgcgtc
      781 aggacgatgt tgctgccacc gccgagcacg aacttcggcg cggtgccgag ctgcgggtgg
      841 tcgacgacgc ggcgcacgtc ggcgtcgctc gcgacgcgca ccagcgtgcc cgccaccgcc
      901 ggcaggccga agctgttgta gggtttgagg ctcacgcccc gctcgatttg catgccacaa
      961 ttttgaccga tggagcccct cgccgagcgt gtacgctcgg cgatcccccg aggggatgcg
     1021 ggccggcttg gctgggccac gaaggagccc ctgcgtggcc cttgcccggc gctcggcccg
     1081 ccaccacctc gaccgtcatc atctttgcga gtcatcgcca tgcccagttt cgacgccgtc
     1141 ctagaaccca acctcgtgga agtgcgcaac gcggtcgacc agaccgcgaa ggagatcggc
     1201 acgcgcttcg atttcaaggg ctcgtcggcg cgcgtcgagc tgaaggaaaa ggagctcacg
     1261 gtctgggccg acagcgactt ccagatcggc caggtgctgg acgtgctgat cgccaagctg
     1321 accaagcgcg gcgtcgacat ccgcttcctc gaccgcagtg cgaagatcga gaagatcggc
     1381 ggcgacaagg tcaggcagcc cctcaccgtg aagagcggca tcgactcgga caccgcgaag
     1441 aagatccaga acttcgtcaa gcagagcaag ctgaaggtgc aggccgcgat ccagggcgac
     1501 acggtgcgcg tgaccggcgc caagcgcgac gacctacagg ccgcgatggc gctgatccgc
     1561 aaggagatcg ccgatgcgcc gctgtcgttc acgaacttcc gcgactgagc gatgcgcggg
     1621 gtcgccgcag tcgcgttgct gctgctggcg gcacccgcct tcgcgcaaag cgtctcgctg
     1681 agcggcagcc tgggcagcaa ggccttgctg gtgatcgacg gcacgccgcg cagcgtcgcg
     1741 gtcggcacca gcgtgcaggg cgtgaagctg gtcgcggtga gcggcagcga cgccgtcgtc
     1801 gagatcaagg gcaagcgcgt gacgctgctc atgggcggcg cgcaggtcaa cctcggcggc
     1861 gcggccagcg agggcacggg ctcgcagatc gtgctcagcg ccgacagcgg cggccacttc
     1921 ttctcgagcg gcgcgatcaa cggcaagccg gcgcacttcc tggtcgacac cggtgcgacc
     1981 ttcgtctcga tgggcgcggc gcaggcgcag cagctcggca tcgacttcgc ccgcggccag
     2041 cgcggcatca gcgacaccgc gaacgggccg atggtcgtct acaaggtcaa gctcgcctcg
     2101 gtgcgcgtcg gcgacgtgct gctctacgac gtcgacgggc tcgtcagcca gcagccgatg
     2161 ggggccgtgc tgctcggcaa cagcttcctg gcccgcttcc agatgaagcg cgagaatgac
     2221 acgatgaccc tgaccagacg ctactgacgc gcgtctgtcg cagcagggac cgcagagaag
     2281 cgcacggtcg tgctaaaacc gcggacgacc tagcaccaac cccgtcgatc agcccggaga
     2341 caagccgcga tggacctcaa tttcacccct gaagagcaag cgttccgcga agagattcgc
     2401 gcctgggtgg cc
//
LOCUS       DATMJB010000092         2316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_758242, whole genome shotgun sequence.
ACCESSION   DATMJB010000092 DATMJB010000000
VERSION     DATMJB010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758242"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /gene="purH"
                     /locus_tag="VNU48_02065"
     CDS             complement(<1..716)
                     /gene="purH"
                     /locus_tag="VNU48_02065"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867393.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWI10093.1"
                     /translation="MTQLTALLSVSDKTGVLDLARALDALGVKLLSTGGTAKLLADAG
                     LPVTEVADHTGFPEMLDGRVKTLHPTIHGGLLARRDLPAHMAALATHGIATIDLLVVN
                     LYPFEATVAKAGCTLEDAIENIDIGGPAMVRSAAKNWKDVAVLTDASQYAPVLAELQA
                     GGVVSAATKFALAVAAFNRISNYDAAISDYLSSLRSDGTRALFPAQSNARFVKLQDLR
                     YGENPHQHAAFYRDLYPAPGS"
     gene            complement(763..1002)
                     /locus_tag="VNU48_02070"
     CDS             complement(763..1002)
                     /locus_tag="VNU48_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI10094.1"
                     /translation="MKKTTHIEECIRESLEIYFKDLRGVEPAAMYEMILGVVEKPLLD
                     VVMKHADGNQSRAADWLGINRNTLRRKLLDHKIIK"
     gene            complement(999..2039)
                     /gene="dusB"
                     /locus_tag="VNU48_02075"
     CDS             complement(999..2039)
                     /gene="dusB"
                     /locus_tag="VNU48_02075"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HWI10095.1"
                     /translation="MQIGHFALANRLFAAPMAGVTDRPFRQLCKRLGAGYAVSEMVTS
                     RRDLWTSLKTSRRADHAGELAPIAVQIAGTDAQMMADAAAYNIDRGAQIIDINMGCPA
                     KKVCNKWAGSALMQDEPLALAIVEAVVAACAPRTSRCAAERNAVRLARAAESAGIAMV
                     TVHGRTREQGYKGLAEYDTVSAVKAALRIPVVANGDIDSPRKARDVLDATGADAIMIG
                     RAAQGRPWIFREVAHFLATGEQLDAPPVTQVKAWLIEHLHDHYGLYGWDDAGAGVRSA
                     RKHIGWAVRALPGGEAFRARMNLLESCETQVRALGEWFDALADTHDLLPRADEAANDS
                     LMIEEESTDKLA"
     gene            2150..>2316
                     /locus_tag="VNU48_02080"
     CDS             2150..>2316
                     /locus_tag="VNU48_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWI10096.1"
                     /translation="MEAWVESMLALLALPKFGLSTVFVIAFVSATLLPLGSEPAVFGL
                     VKLNPEMFWPA"
BASE COUNT          313 a          787 c          818 g          398 t
ORIGIN      
        1 gaacccggcg cggggtacag gtcgcggtag aacgccgcgt gctggtgcgg gttctcgccg
       61 tagcgcaggt cctgcagctt gacgaagcgc gcgttcgact gcgccgggaa cagcgcgcgc
      121 gtgccgtcgg agcgcagcga cgacaggtag tcgctgatcg ccgcgtcgta gttcgagatg
      181 cggttgaacg cggccactgc gagtgcgaac ttcgtcgccg cgctgaccac gccgccggcc
      241 tgcagctcgg ccagcaccgg cgcgtactgc gacgcgtcgg tcagcaccgc gacgtccttc
      301 cagttcttcg ctgccgagcg caccattgcc ggcccgccga tgtcgatgtt ctcgatcgca
      361 tcttcgagcg tgcagccggc cttcgcgacg gtggcctcga acgggtagag gttgaccacc
      421 agcaggtcga tcgtcgcgat cccgtgcgtt gccagcgcag ccatgtgcgc cggcaggtcg
      481 cggcgcgcca gcaggccgcc gtggatcgtc gggtgcagcg tcttgacgcg gccgtcgagc
      541 atctccggga agccggtgtg atcggcgacc tcggtgaccg gcaggccggc gtcggcgagc
      601 agcttcgcgg tgccgccggt cgacagcagc ttcacgccga gcgcatcgag cgcacgggcc
      661 aggtcgagca cgccggtctt gtcggagacc gacagcaatg cggtgagttg tgtcatggga
      721 gccctgtcgc cgatcacgtc ctcgcgcagc gaggcgtatc agtcatttga tgatcttgtg
      781 gtcgagcagc ttgcggcgca gcgtgttgcg gttgatgccc agccagtcgg ccgcgcggct
      841 ctggttgccg tcggcatgct tcatcaccac gtccagcagc ggtttctcga ccacgccgag
      901 gatcatctcg tacatggccg ccggttcgac gccgcgcagg tccttgaagt agatctcgag
      961 actctcgcgg atgcattctt cgatgtgggt cgtcttcttc atgccagctt gtcggttgat
     1021 tcttcttcga tcatcagcga atcgttggcg gcctcgtcgg cgcgcggcag caggtcgtgc
     1081 gtgtcggcga gcgcgtcgaa ccattcgccg agcgcgcgca cctgggtctc gcagctctcg
     1141 agcaggttca tgcgggcacg aaacgcctcg ccgcccggca gcgcgcgcac cgcccagccg
     1201 atgtgcttgc gggcgctgcg cacgccggcg ccggcgtcgt cccagccgta cagaccgtag
     1261 tgatcgtgca gatgctcgat cagccaggcc ttgacctgcg tcacgggcgg cgcatcgagc
     1321 tgctcgccgg tggcgaggaa gtgcgcgacc tcgcgaaaga tccacggccg gccctgcgcg
     1381 gcgcggccga tcatgatcgc gtcggcgccg gtggcgtcga gcacgtcacg cgccttacgc
     1441 ggcgaatcga tgtcgccgtt ggcgaccacc ggaatgcgca gggccgcctt caccgcgctg
     1501 acggtgtcgt attcggccag ccccttgtag ccttgctcgc gcgtgcggcc gtgcaccgtg
     1561 accatcgcga tgccggccga ctctgccgcg cgcgccagcc gcaccgcgtt gcgctcggcc
     1621 gcgcaccggc tggtgcgcgg cgcgcaggcg gcgaccaccg cctcgacgat cgccagcgcc
     1681 agcggctcgt cctgcatcag cgccgagccg gcccacttgt tgcacacctt cttcgccggg
     1741 cagcccatgt tgatgtcgat gatctgcgcg ccgcggtcga tgttgtaggc cgcggcgtcg
     1801 gccatcatct gcgcgtcggt gccggcgatc tgcaccgcga tcggcgcgag ctcgccggcg
     1861 tggtcggcgc gccggctggt cttcaggctg gtccacaggt cgcgccgcga ggtcaccatc
     1921 tcgctgaccg catagcccgc gcccaggcgc ttgcagagct ggcggaacgg ccggtccgtg
     1981 acgccggcca tcggggcggc gaagaggcgg ttcgccagag cgaagtggcc gatctgcatg
     2041 gggcgggggg ctgcgggcgg atgctgaaaa aggaggcaga agtatagcgg cggcgcggcg
     2101 cgcgacatcc cactgcgcgg gccggccgcg ctggccataa tccgctgcga tggaagcatg
     2161 ggtcgaatcg atgttggcgc tgctggccct gccgaagttc ggcctgagca cggtgttcgt
     2221 catcgccttc gtctcggcca cgctgctgcc gctgggttcc gagcccgcgg tgttcggcct
     2281 cgtcaagctg aaccccgaga tgttctggcc ggccat
//
LOCUS       DATMJB010000093         2624 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_816911, whole genome shotgun sequence.
ACCESSION   DATMJB010000093 DATMJB010000000
VERSION     DATMJB010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816911"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(277..2529)
                     /locus_tag="VNU48_02085"
     CDS             complement(277..2529)
                     /locus_tag="VNU48_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWI10097.1"
                     /translation="MPTHTQPATTDQPHPPNPRHAQRLPAQLGKEQFNRIRQAAYTLP
                     QVVKPGDYLDERSAGFEDHLDKLGLVLLQKPVAGARPSRWIEPALMGSALAHLDAVGT
                     GPGVRPLMLERTAALEALGVSRRAGQWTRLISHALQLPDPPWRVPVLFRVPVEADAVW
                     AFYRGGDAAALRAALGAPPAYPHAAELIAHLEALIARSRDYKPAHLDSLLPRLQSEYA
                     VPQALDDSQSLAELKAACSRAQDRWEHQVTELDTLAGLNSELAFQGYPDTFQKARHLQ
                     RSVTLYVGPPNSGKTHAAFERLAQALDGAYLAPLRLLALEGRDRLVARGVPTSLLTGE
                     ENVPADGARVVSSTIEMVGTNTPIDVAVIDEAQMIFDPSRGWAWTQAIVAVPANEVII
                     ICSAYAVPAIENLLGLCGERCTVKHFDRKQHVQLLGAPVPTAALKLGDAIVAFSRRDV
                     LMLRDQIAAGGHPVSVIYGALPPEVRRREAERFAQGESHILVATDAIGMGLNLPIRRV
                     LFSTMSKFDGVGDRPLNESEVHQIAGRAGRYGMHEEGFVGVLKEAEPAAARMLKELLH
                     KTPRAPDDFKAPVAPNGWHVDTIASRLHKTKLREVLGVFVEQLKLDDAHFEVAELDQM
                     LELAEQLDRVAAKLTLKERFIYAQAPVDTRTETQVQAYLDWSASHALTGQAGSPWFLD
                     DVDSHSRLDRMEQALRACTLWLWLDLRFPGVYGHVERVIALRGSLNDGIERQLKGKRP
                     LAMVRRGAKR"
BASE COUNT          348 a          905 c          959 g          412 t
ORIGIN      
        1 cgctgaccca aagccggtca tcaccttggc gtcggggtgc ttgccgcccg cctgtgccag
       61 gtcgatggcg tgcccgaaca cgtctttgac gtcctcgggc atggcatcca ggtcgcgctt
      121 ggcggaaccg acccacttga cgggtttgcg aggccgcttc gacatcaaag gcattatgcc
      181 aattttggca tgcccgcgcg ctttgggcgc aggccgagga gatgcgcaag cccaacgcga
      241 gacgccggat ggccgacccg gctgcgcagg cgccgctcac cgcttcgcgc cgcgccgcac
      301 catcgccagc ggccgcttgc ccttcagctg ccgctcgatg ccgtcgttca ggctgccgcg
      361 cagcgcgatg acgcgctcga cgtggccgta gacgccgggg aagcgcaggt ccagccacag
      421 ccacagcgtg cacgcgcgca gcgcctgctc catgcggtcg agccggctgt ggctgtcgac
      481 atcgtccagg aaccacgggc tgccggcctg gccggtgagc gcgtgactgg cgctccagtc
      541 gaggtaggcc tgcacctgcg tctcggtgcg cgtgtcgacc ggcgcctgcg cgtagatgaa
      601 gcgctccttc aatgtgagtt tcgcggcgac gcggtcgagc tgctcggcga gctcgagcat
      661 ctggtcgagc tcggcgacct cgaagtgcgc gtcgtcgagc ttgagctgct cgacgaacac
      721 gcccagcacc tcgcgcagct tcgtcttgtg cagacgcgat gcgatcgtgt cgacatgcca
      781 gccgttcggc gccaccggcg ccttgaagtc gtcgggcgcg cgcggcgtct tgtgcagcag
      841 ctccttcagc atgcgcgcgg ccgcgggttc ggcctccttc agcacgccga cgaagccctc
      901 ctcgtgcatg ccgtagcggc cggcgcggcc ggcgatctgg tgcacctcgg attcgttcag
      961 cggtcggtcg ccgacgccgt cgaacttgct catcgtgctg aacagcacgc gccggatcgg
     1021 caggttcagc cccatgccga tcgcgtcggt cgcgaccagg atgtgcgact cgccctgcgc
     1081 gaagcgctcg gcctcgcggc gccgcacctc gggcggcagc gcgccgtaga tcaccgagac
     1141 cgggtggccg ccggcggcga tctggtcgcg cagcatcagc acgtcgcggc ggctgaacgc
     1201 gacgatcgcg tcgccgagct tgagcgcggc ggtcggcacc ggcgcgccga gcagctgcac
     1261 gtgctgcttg cggtcgaagt gcttgacggt gcagcgctcg ccgcagaggc caagcaggtt
     1321 ctcgatcgcc ggcaccgcgt aggccgagca gatgatgatc acctcgttgg ccggcaccgc
     1381 gacgatcgcc tgggtccagg cccagccgcg cgacgggtcg aagatcatct gcgcctcgtc
     1441 gatcacggcc acgtcgatcg gcgtgttggt gccgaccatc tcgatcgtgc tgctgacgac
     1501 acgtgcgccg tcggccggca cgttctcctc gccggtcagc agcgaggtcg gcacgccgcg
     1561 cgcgaccagc cggtcgcgcc cttcgagcgc gagcagccgc agcggcgcga ggtaggcgcc
     1621 gtcgagcgcc tgcgccagac gctcgaacgc ggcgtgggtc ttgccgctgt tcggcgggcc
     1681 gacgtacagc gtcacgctgc gctgcagatg gcgcgccttc tggaacgtgt cggggtagcc
     1741 ctggaacgcg agctcgctgt tcaggccggc cagcgtgtcg agctcggtga cctggtgttc
     1801 ccagcggtcc tgcgcgcgac tgcaggcggc cttcagttcg gccaacgact gcgagtcatc
     1861 gagtgcctgc ggcacggcgt attcggattg caggcgcggc agcagcgagt ccaggtgcgc
     1921 aggcttgtag tcgcggctgc gcgcgatcaa cgcctcgagg tgcgcgatca gctcggccgc
     1981 gtgcgggtag gccggtggcg cgccgagcgc ggcgcgcagc gcggccgcgt cgccgccgcg
     2041 gtagaaggcc cagaccgcgt cggcctcgac cggcacgcgg aacagcaccg gcacgcgcca
     2101 gggcgggtcg ggcagctgca gcgcgtggct gatcagccgt gtccactggc cggcgcggcg
     2161 cgacacgccg agcgcctcga gcgcggccgt gcgctcgagc atcagcgggc gcacgcccgg
     2221 gccggtgccg acggcgtcga gatgcgccag cgcgctgccc atcagcgccg gctcgatcca
     2281 gcggctcggg cgcgcgccgg cgacgggttt ctgcagcagc acgaggccga gcttgtcgag
     2341 gtggtcctcg aagccggcgc tgcgttcgtc gaggtagtcg cccggcttca cgacctgggg
     2401 cagcgtgtac gcggcctggc ggatgcggtt gaactgctcc ttgccgagct gcgcgggcag
     2461 gcgctgcgca tggcgcgggt tcggcgggtg gggctggtcg gtggtggcgg gttgtgtatg
     2521 tgttggcatt gccccgattg tcgcggactt gactttgacc gcaccgccca aaggtgggga
     2581 gcatgggttc gcgaagccgg gccccttgca ggagcacacc atgt
//
LOCUS       DATMJB010000094         2778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_283670, whole genome shotgun sequence.
ACCESSION   DATMJB010000094 DATMJB010000000
VERSION     DATMJB010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283670"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VNU48_02090"
     CDS             <1..475
                     /locus_tag="VNU48_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HWI10098.1"
                     /translation="AMPAAMQSVVERIGENCEPSLTTVLFLGGAGGSLRAGVTDNPVR
                     LTRSIKQALVNVTCGGAPAYVWPGGGITVMVDVMRMPDQSFGTVPTPAIVAPIEFSMR
                     LDDYRALGGHMEHVRSLTDVLARGAWHNDGAPTARRYEALPGANPWPLAQPPLLG"
     gene            489..1394
                     /locus_tag="VNU48_02095"
     CDS             489..1394
                     /locus_tag="VNU48_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HWI10099.1"
                     /translation="MQAQRARLPNGRWHFQHGPIDLVIGADGVADAVAAAHEAAWRRF
                     ETALPELVAELRVLRLAVVGGPCPLQGAIARRMWHACRPYRAPFITPMAAVAGAVAQE
                     VLGLYERAGIARAWVNNGGDIALHLAPGASVKVGLVADLASVDPAEGAPRIDADFAVD
                     AALPVRGIATSGWRGRSFSLGIADSVTVLARNAAMADAAATVIANAVDVDDPRIVRRP
                     ANELKDDTDLGAIPVTVDVPPLAAAQVQAALRSGARRARELQGAGLIHAAVLVCQHRA
                     LGVEARVGPAPLAGTAAAELGSAVA"
     gene            1456..2106
                     /locus_tag="VNU48_02100"
     CDS             1456..2106
                     /locus_tag="VNU48_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HWI10100.1"
                     /translation="MIEVRRVFTQVEDIHHEFGPVAATPLRRGAIGVVLTNPFAGRYV
                     ADILPMMDALQPLGIEMAQRLRAAMDVPVGRIEGYGKGAIVGANGELEHGALWHVPGG
                     YAMRELLGWKGDRAAYAQGRVPAAGQAGAQTGNALAIVPSTKKVGPPGAALDVPLTHI
                     NAAYVRSHFDAIEVRVPGSPLADEIVVILAMSTGPRVHARVGGLKAADIAQWNGLR"
     gene            2125..2709
                     /locus_tag="VNU48_02105"
     CDS             2125..2709
                     /locus_tag="VNU48_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HWI10101.1"
                     /translation="MLAKIRKLIVQVDETRIEMGKAVTPPTRRALAMAVIANPCAGRY
                     EEKLDELIAIGEELGGLLGDKCIAALGIAPGQAQSYGKAAIVGEAGELEHAAAILHPK
                     LGAPLRRAVEKGAALVPSAKKRGGLGTAIDVPLGHKDAAFVRSHFDAMEARVSDAPRA
                     NEIVVAVVVTDSGRPLPRIGGLQAHEVKGEDGLR"
BASE COUNT          394 a          983 c         1034 g          367 t
ORIGIN      
        1 cgcgatgccg gcggcgatgc agtcggtcgt cgaacgcatc ggcgagaact gcgagccctc
       61 gctgacgacg gtgctgttcc tcggcggcgc cggcggcagc ctgcgcgccg gcgtcaccga
      121 caacccggtg cggctgacgc gctcgatcaa gcaggcactc gtcaacgtga cctgcggcgg
      181 tgcgccggcc tacgtgtggc cgggcggcgg catcaccgtg atggtcgacg tgatgcgcat
      241 gcccgaccag agcttcggca ccgtgccgac gccggcgatc gtcgcgccga tcgagttcag
      301 catgcggttg gacgattacc gcgcgctggg cggccacatg gagcatgtgc gcagcctcac
      361 cgatgtgctc gctcgcggcg cctggcacaa cgatggcgcg cccaccgcgc gccgatacga
      421 ggcgctgccg ggcgcgaacc cgtggccgct ggcgcagccg ccgctgctcg gctgagcgcg
      481 acgacgccat gcaggcacaa cgcgcgcgtc tgccgaacgg ccgctggcat ttccagcacg
      541 gcccgatcga cctcgtgatc ggcgccgacg gtgttgccga cgcagtcgct gccgcgcacg
      601 aggccgcgtg gcggcgcttc gagaccgcgt tgcccgagct cgtggccgaa ctgcgcgtgt
      661 tgcgcttggc ggtggtgggc ggcccctgcc cgctgcaagg cgcgatcgcg cgccgcatgt
      721 ggcacgcctg ccggccgtac cgcgcaccct tcatcacgcc gatggcggcg gtcgcgggcg
      781 cggtggcgca ggaagtgctc ggcctgtacg agcgcgcggg catcgcccgc gcctgggtca
      841 acaacggcgg cgacatcgcg ctgcacctcg caccgggtgc atcggtgaag gtcggcctgg
      901 tcgccgacct ggcgagtgtc gacccggccg agggcgcgcc gcgcatcgat gccgatttcg
      961 cggtcgacgc ggcgctgccg gtgcgcggca tcgcgaccag cggctggcgc ggccgcagct
     1021 tctcgctggg catcgccgac agcgtcaccg tgctggcccg caacgcggcg atggccgacg
     1081 cggccgcgac cgtgatcgcg aacgccgtcg atgtcgacga cccgcgcatc gtgcgccggc
     1141 ccgcgaacga actgaaggac gacaccgacc tcggcgcgat cccggtcacg gtcgacgtgc
     1201 cgccgctggc cgccgcgcag gtgcaggcgg cgctgcgcag cggggctcgg cgcgcgcgcg
     1261 agctgcaggg cgcgggcctg attcacgccg cggtgctggt gtgccaacat cgcgctctcg
     1321 gcgtcgaggc gcgggtcggg ccggcgccgc tcgcgggcac cgcggccgcc gaacttggtt
     1381 cagcagttgc ttaatgaaaa tcgccatgcc agcaccgatt cgtcacgtcg cccgtcagcg
     1441 ccggcggagc gcgccgtgat cgaggtccgc cgtgtcttca cgcaggtcga ggacatccac
     1501 cacgagttcg gcccggtggc ggccacgccg ctgcggcgcg gcgcgatcgg cgtggtgctg
     1561 acgaacccgt tcgccggccg ctacgtcgcc gatatcctgc cgatgatgga cgcgctgcag
     1621 ccgctcggca tcgagatggc gcagcggctg cgcgcggcga tggacgtgcc ggtcggacgc
     1681 atcgagggct acggcaaggg tgcgatcgtc ggcgcgaacg gcgagctcga gcacggcgcg
     1741 ctgtggcacg tgccgggcgg ctacgcgatg cgcgagctgc tcggctggaa gggcgatcgc
     1801 gccgcgtatg cacagggtcg ggtgcccgcc gccggccagg ccggcgcgca gaccggcaat
     1861 gcgctcgcga tcgtgccgtc gacgaagaag gtcggcccgc cgggcgccgc gctcgacgtg
     1921 ccgctgaccc acatcaacgc cgcctacgtg cgcagccact tcgacgcgat cgaggtgcgc
     1981 gtgcccggca gcccgctggc cgacgagatc gtggtcatcc tcgcgatgag caccgggccg
     2041 cgcgtgcatg cgcgcgtcgg cggcctgaag gccgccgaca tcgcgcagtg gaacggcctg
     2101 cgctgacgag acaaggatag agacatgctc gcgaagatcc gaaaactcat cgtccaggtc
     2161 gacgaaaccc gcatcgagat gggcaaggca gtgacgccgc cgacgcgccg cgcgctcgcg
     2221 atggcggtga tcgcgaaccc gtgcgccggc cgctatgaag agaagctcga tgaattgatt
     2281 gcgatcggcg aagagctcgg cggcctgctc ggcgacaagt gcatcgcggc gctcggcatc
     2341 gcgccgggcc aggcgcagag ctacggcaag gcggcgatcg tcggcgaggc cggcgaactc
     2401 gagcacgccg cggcgatcct gcaccccaag ctcggcgcgc cgctgcgccg cgcggtcgag
     2461 aagggcgcgg cgctggtgcc gtcagcgaag aagcgcggcg gcctgggcac cgcgatcgac
     2521 gtgccgctgg gccacaagga cgcggctttc gtgcgcagcc atttcgatgc gatggaagca
     2581 cgcgtgtccg atgcgccgcg cgccaacgag atcgtcgtcg cggtcgtcgt gaccgacagc
     2641 ggccggccgc tgccgcgcat cggcgggctg caggcgcacg aggtcaaggg cgaggacggg
     2701 ctgcgctgac gcacgcgccg caacagtcgc tgtcgccgta cccgccgttc tattcaccgt
     2761 tgccatactc gccgtcgt
//
LOCUS       DATMJB010000095         3614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_697763, whole genome shotgun sequence.
ACCESSION   DATMJB010000095 DATMJB010000000
VERSION     DATMJB010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697763"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1804
                     /locus_tag="VNU48_02110"
     CDS             137..1804
                     /locus_tag="VNU48_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWI10102.1"
                     /translation="MSQALAPIRDQLVRIALLMALGLAVASLAGTLLARRMLIPITAL
                     RAGARRLGEGDFGHRIEVKTSDELEELANQFNSMAGQLHETYTDLESKVKERTRDLAQ
                     SINELKVLEEVGRAVSSSLDLNAVLPTVASRALDITHADAVLIYGYDAARRQFSLTEA
                     IGIDKSAEGEHRAIDGSASVLGEAAAKGEPIAIPDLDHAADNPLRGLVMTAGFHSVLV
                     VPLVDQTGVLGSLVVLRKEAGEFSPNLIGLMRTFAHQSVLAMRNARLFTEVDHKGREL
                     AVAHSTVQQQAGKLQEQTDQLLNWNKSLEERVEKQLGEIERIRRLERFLAPQVAQLIA
                     SSDGHDSLLDSHRREVTVVFCDLRGFTSFTESTEPEEAMAVLREYHAALGELIFRYEG
                     TLDRYAGDGVMILFNAPIQFEDHTRRAVKMAVEMRDSIGMLTEKWRNRGHSLGFGVGI
                     ALGYATLGQIGFEQRLEYAAIGSVTNLASRLCDEAKANQIVVSRRVYGVVEPWVEARS
                     IDDLTLKGFNHPVLAMEILRWKEDVAAAVSPAQATPPAKERRRKQPS"
     gene            complement(1885..2649)
                     /locus_tag="VNU48_02115"
     CDS             complement(1885..2649)
                     /locus_tag="VNU48_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10103.1"
                     /translation="MASFMRLRQICLVAPQLEPLIGDISAIMGLNVCYRDGTLAKYGL
                     ENALLPVDTILLEVVAPFQPGTAAGRFIEKTGGRGGYMAIFCCDDPDERARHAKAMGV
                     RTANVIDHASYHGVQLHPRDCRAAFIELNHTAGSDNISGPYPPAGPDWQKSIRKDVTQ
                     ALNGVEMESPEPQVLAEHWGKIIGVAASKNQNGEPELKLPNCSFRFVKGPVDIMSGLT
                     FEVADAGKVREAARARGYAVSGDGFAIGGVTFSLVA"
     gene            complement(2652..3440)
                     /locus_tag="VNU48_02120"
     CDS             complement(2652..3440)
                     /locus_tag="VNU48_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWI10104.1"
                     /translation="MNDMVLQKLDAGLLTITMNRPDRRNALNADMTRGLVEAARRAAE
                     DPEVRAVLLKGAGGTFCVGGDVKSMAEGQTPQTFEAKMVQLRRGMEVSRILHQMAKPV
                     VAQIDGAAAGAGLSIALSCDLRVASASAKITTAFAKVGLSGDYGGTYFLTHLLGSAKA
                     RELYLTSPILSAQEAYHLGIVTRVVPDVEIDAAAHDLAMSLAQGPSVTLGYIKKNINN
                     AEQMSLEECFDAEAMHHSRSGETADHKEAAKAFVEKRKPAFQGR"
BASE COUNT          640 a         1160 c         1179 g          635 t
ORIGIN      
        1 gctgccgcag gtcgcggcgg tgatggcgcc cggcggcgtg gcgccggcat cgggcaccga
       61 cagcaacggc gccgcggtgc tgaccacggc gaatgccgtg cccaaactag gctggttcgt
      121 gttcttcgag cagccgatgt cgcaggcgct ggcgccgatc cgggatcaac tggtacggat
      181 tgcgctgctg atggcgctcg ggctcgcggt cgcgagcctc gccggcacgc tgttggcgcg
      241 ccgcatgctg attccgatca cggcgttgcg cgccggtgcg cggcggctcg gcgaaggcga
      301 tttcggccat cgcatcgagg taaagacttc tgacgaactg gaagagctgg cgaaccagtt
      361 caacagcatg gccggacagt tgcacgagac ctataccgac ctcgaatcga aggtaaagga
      421 acggacccgc gatctcgcgc agtcgatcaa cgaactcaag gtgctggagg aagtcggccg
      481 cgcggtgtcg tcctcgctcg atctcaacgc cgtgctgccg acggtggcgt cgcgcgcgct
      541 cgatatcacc cacgccgacg cggtgctgat ctacggctat gacgcggcca ggcgtcaatt
      601 cagcctgacc gaagcgatcg gcatcgacaa atcggccgaa ggcgagcatc gcgccatcga
      661 cggcagcgcc agcgtgctcg gtgaagccgc ggcgaagggc gagccgatcg cgatccccga
      721 tctcgatcat gccgcggaca atccgttgcg gggcctcgtg atgacagccg gtttccattc
      781 ggtgctggtg gtgccgctgg tggaccagac cggagtgctg ggatcgctgg tggtgctgcg
      841 caaggaggcg ggcgaattct cgccgaacct gatcggcttg atgcggactt tcgcgcatca
      901 atcggtgctg gcgatgcgta acgcgcggct gtttaccgaa gtcgaccaca agggccgcga
      961 actggcggtg gcgcattcca ccgtgcagca gcaggccggc aagctgcagg agcagaccga
     1021 ccagttgctc aactggaaca agtcgctgga agagcgggtc gagaagcaac tcggcgagat
     1081 cgagcgcatc cgccgcctcg aacgtttcct ggcgccgcag gtggcgcagc tgatcgcttc
     1141 ttccgacggc cacgattcgc tgctggacag ccaccgacgc gaagtcaccg tggtgttctg
     1201 cgatctcaga ggttttacct cgttcaccga aagcaccgag ccggaagagg cgatggccgt
     1261 gctgcgcgag taccacgccg ccctcggcga actgattttc cgctacgagg gcacgctcga
     1321 ccgctatgcc ggcgacggcg tgatgatcct gttcaacgcg ccgatccagt tcgaggatca
     1381 tacccggcgc gcggtgaaga tggcggtcga gatgcgcgac agcatcggga tgctgacgga
     1441 gaagtggcgc aaccgcggcc acagcctcgg tttcggcgtc ggcatcgcgc tcggctatgc
     1501 cacactgggc cagatcggct tcgagcagcg gctggaatac gccgcgatcg gcagcgtcac
     1561 caacctcgcc tcgcggctgt gcgacgaagc caaggcgaat cagatcgtgg tctcgcggcg
     1621 cgtgtacggc gtggtcgaac catgggtcga ggcgcgctcg atcgacgacc tgacgctgaa
     1681 gggcttcaat catccggtgc tggcgatgga gatcctgcgc tggaaggaag atgtcgcagc
     1741 ggccgtgtca cccgcgcagg cgacgccgcc ggcaaaggaa cggcgacgga agcagccgtc
     1801 gtgagtatcg tttcgcctcg cccgcttacg gcccgcgagc gggagaggca agacccagcc
     1861 atatagcccg gttgaatcgc cggctcacgc caccagacta aacgtcaccc cgccgatcgc
     1921 aaacccgtcg cccgacaccg cgtagcccct ggccctggcg gcctcgcgca cttttcccgc
     1981 gtcggcgacc tcaaacgtca ggccgctcat gatatcgacg gggcccttga cgaagcggaa
     2041 gctgcaattg ggcagcttca gttccggctc gccgttctgg ttcttcgaag cggcaacgcc
     2101 gatgatcttg ccccaatgct cggccagaac ctgcggttcc ggactctcca tttccacacc
     2161 gttcaacgcc tgcgtcacat ccttgcggat cgacttttgc cagtcgggac cggccggcgg
     2221 atagggaccg gaaatgttgt cgctgccggc ggtgtgattg agctcgatga acgccgcgcg
     2281 gcaatcgcgc ggatgcagct gcacgccgtg ataggacgcg tgatcgatga cattggcggt
     2341 gcgcacgccc atcgccttgg catgacgggc tcgctcatcg ggatcgtcac agcagaagat
     2401 cgccatgtag ccgccgcggc cgccggtctt ttcgatgaaa cgtcccgccg cggtgccggg
     2461 ctggaacggc gccaccactt ccagcaggat ggtatcaact ggcagcagcg cgttctccag
     2521 gccgtatttg gccaaagtgc cgtcgcgata gcagacattg agccccatga tcgcggagat
     2581 atcgccgatc agcggctcaa gttgcggcgc caccaggcag atctgccgca gcctcatgaa
     2641 gctggccatc ttcaacggcc ctggaaagcg ggcttgcgct tctcgacgaa cgccttggcg
     2701 gcttccttgt gatcggcggt ctcgccggag cgcgagtgat gcatcgcctc ggcgtcgaag
     2761 cactcctcga gcgacatctg ctccgcgtta ttgatgttct tcttgatgta gccgagcgtc
     2821 accgacggcc cctgcgccag cgacatcgct aggtcatgcg cggcggcatc gatttcgacg
     2881 tcgggtacga ccctggttac gatgccgaga tgataggctt cctgcgcgct caggatcggc
     2941 gaggtcagat aaagctcgcg cgccttggcg ctgccgagca gatgggtcag gaaataggtg
     3001 ccgccgtaat cgcccgacag accgaccttg gcaaaggcgg tggtgatctt ggccgacgcg
     3061 ctggcgacgc ggagatcgca ggacagcgca atcgatagac ccgcgcccgc cgccgcgccg
     3121 tcgatttgcg ccacaaccgg cttcgccatc tggtgcagga tccgcgacac ttccatgccg
     3181 cggcgaagct ggaccatctt ggcttcaaac gtctgcggcg tctgtccctc cgccatcgac
     3241 ttcacatcgc cgccgacaca gaacgtgccg cccgcgccct tgagcagcac ggcgcggacc
     3301 tccggatcct cggccgcgcg ccgcgccgcc tcgaccagcc ctcgcgtcat gtccgcattg
     3361 agcgcattgc gccggtccgg ccggttcatg gtgatggtga gcaggccggc atcgagtttc
     3421 tgaagaacca tgtcgttcat gaggtatcgc ctttgttgtt attggttgcg agtgaatttc
     3481 aattgtcatt ccgggcgcgc gagcgaatcc ggaacgacgc ttcgcgtcac ttctttacca
     3541 gcggacagcg cgacagcgcg agcggctgga acgcctcgtt gccggaatgg gccagcagct
     3601 tcgcattgat tccg
//
LOCUS       DATMJB010000096         3826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_816523, whole genome shotgun sequence.
ACCESSION   DATMJB010000096 DATMJB010000000
VERSION     DATMJB010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3826
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816523"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1075
                     /locus_tag="VNU48_02125"
     CDS             182..1075
                     /locus_tag="VNU48_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWI10105.1"
                     /translation="MRPTLLETLSLLVLVAPGFALAQAGSTLDKVKAAGAITLAYRES
                     SIPFSYLDDKAQPVGFAHEICLKIVDEVKKAVGKADLKVNLQSVTSANRIPLLQNGTI
                     DIECGSTTNNSERGKQVQFATNYFYTGTRFLVKTGTQVKTIDDLKGKTVVSTSGTTNY
                     KILRNLNEEKKLGIDLLAAKDHAESALMVESGRAVAFGMDDILLYGLRASAQNPASLA
                     VVGEPIQVEPYAIMIRLNDPAFKKLVDDTLAGLMKSGEFTKLYTKWFQSPIPPKGINL
                     NAPMSQELIDNMKALSDKPAT"
     gene            1123..1827
                     /locus_tag="VNU48_02130"
     CDS             1123..1827
                     /locus_tag="VNU48_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10106.1"
                     /translation="MTITAAHRRRLREVYRSAGWPCQDALELELLAAGLLERQRSALG
                     HETVRVSDAGIALLAQTLQRNRARRDAHELLVERVAREMTRAGRIAWRGLSLRAKVGE
                     AWAVAMPDVFSIRHTSVEAYLQPVVHEIKVNRADLLSDLRSAAKRAAYLQLSSECWYV
                     IRAGIAAPEEIPIECGVLVALDAALEVARPAPKRSMQMPFGLWMALARATPLEGWRLE
                     DAQGQLGDTSQSSPDL"
     gene            1838..3469
                     /locus_tag="VNU48_02135"
     CDS             1838..3469
                     /locus_tag="VNU48_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWI10107.1"
                     /translation="MFTQPRHALTALALTALLAACSKPAEVAAPAAPQAPVAAPADHA
                     DSGIAWKQASTDADVDAAFAQAKAQTKPVFVYWGAKWCPPCNQVKATLFNRRDFIERS
                     RAFVPVYVDGDSPGAQKLGSRFKVSGYPTMLLFNPQGVEMTRLPGEVEAERYTQVLTL
                     GMNAQRPVRDLLAQVKAGGAGLTANDWRLLAFYSWDTDQQQLVTKDGVPALMKQLADA
                     CPADQTEIATRLRLKALAAVDDKAPARPDAAAQATLLKVLADAASARPYMDVLTNYAT
                     DITRALSARGSPQRAELLAAFDAALKRFEADETLSRADRMTALIAQVDLATIDAPRGD
                     GAKAAKPKLSDALLADIRAQTTRADREITDGYERQSVITAAAYLLERAGLGGESDALL
                     QANLAKSHSPYYLMSELASNAKARGDKTEALRWYEQAFDKSEGPATRLQWGASYVSAL
                     VEMAPHDDARIEKAAAQLLTEAARQPDAFYERSGRALQRVGAKLQRWNKGGAHAATLK
                     RLQAQLDGVCGALDASDPKRANCAGLFKPAPKQAA"
     gene            3536..>3826
                     /locus_tag="VNU48_02140"
     CDS             3536..>3826
                     /locus_tag="VNU48_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI10108.1"
                     /translation="MPKPPAASDDSNFSAARRRALSLAAAALGARALPGFAAARGDDA
                     TGETGPVVGVWTHAYAAFGRPKYPRDYANFEYVNPAAPKGGTLYLRNPDRRSS"
BASE COUNT          644 a         1372 c         1317 g          493 t
ORIGIN      
        1 gatctacagg cggccagcgc gagcagcagc gcggtcttct tcaacaaggt catggcgagt
       61 ggctcctgga caggtcgggt ggttcgggat cgcgcaaatg ctagcgggaa acccgtcttg
      121 cccgaaattt gcttgatgct aatttcacgt tcctgttcca actttcgccg aaggaccctc
      181 catgcgcccg accctcctcg agaccctttc gctgctggtg ctcgtcgcgc ccggcttcgc
      241 actggcacag gccggctcga cgctcgacaa ggtcaaggcc gccggcgcga tcacgctggc
      301 ctaccgcgag tcgtcgatcc cgttctcgta cctggacgac aaggcccagc cggtcggctt
      361 cgcgcacgag atctgcctga agatcgtcga cgaggtgaag aaggccgtcg gcaaggccga
      421 cctgaaggtc aacctgcaat cggtgacctc ggcgaaccgc atcccgctgc tgcagaacgg
      481 cacgatcgac atcgagtgcg gctcgacgac caacaacagc gagcgcggca agcaggtgca
      541 gttcgcgacc aactacttct acaccggcac gcgcttcctg gtgaagaccg gcacccaggt
      601 caagaccatc gacgacctga agggcaagac cgtggtctcg accagcggca ccacgaacta
      661 caagatcctg cgcaacctga acgaggagaa gaagctcggc atcgacctgc tcgccgcgaa
      721 ggaccacgcc gagtcggcgc tgatggtcga gtcgggccgg gcggtcgcgt tcggcatgga
      781 cgacatcctg ctgtacggcc tgcgtgccag cgcgcagaac ccggcgtcgc tggcggtcgt
      841 cggcgagccg atccaggtcg agccctacgc gatcatgatc cggctgaacg acccggcctt
      901 caagaagctg gtcgacgaca cgctggccgg cctgatgaag agcggcgagt tcacgaagct
      961 gtacaccaag tggttccagt cgccgatccc gcccaagggc atcaacctga acgcgccgat
     1021 gagccaggag ctgatcgaca acatgaaggc gctgagcgac aagccggcga cctgaccgct
     1081 ggtgcgccgc ccgcgcggcg ctacgcttcg cggatggcgg acgtgacgat caccgcggcg
     1141 caccggcgcc gccttcgcga ggtctatcgc tcggccggct ggccgtgcca ggacgcgctc
     1201 gaactcgagc tgctcgctgc cgggctgctc gagcgccagc gcagcgcact gggccacgag
     1261 accgtgcgcg tcagcgacgc cggcatcgcg ctgctggcgc aaacgctgca gcgcaaccgc
     1321 gcgcggcgcg acgcgcacga gctgctggtg gaacgcgtgg cgcgcgagat gacgcgtgcc
     1381 gggcgcatcg cctggcgcgg cctgagcctg cgcgcgaagg tcggcgaggc ctgggcggtc
     1441 gcgatgcccg atgtgttctc gatccgccac accagcgtcg aggcctatct gcaaccggtc
     1501 gtgcacgaga tcaaggtgaa ccgcgccgac ctgctgtcgg acctgcgcag cgcagcgaag
     1561 cgcgccgcct acctgcagct cagcagcgaa tgctggtacg tgatccgcgc cggcatcgcc
     1621 gcgccggaag agatcccgat cgagtgcggg gtgctggtcg cgctcgacgc tgcactcgag
     1681 gtcgcgcggc ccgcgccgaa gcgctcgatg cagatgccgt tcgggctgtg gatggcgctg
     1741 gcccgggcca cgccgctcga agggtggcgg ctcgaggacg cgcagggcca actcggcgac
     1801 acttcgcagt cttcgcccga cctctgaccc gcgccccatg ttcacccagc ctcgacacgc
     1861 gctgaccgcc ctcgcgctga cggctctgct cgcggcctgc tcgaagcccg ccgaggtggc
     1921 cgcgcccgcc gcgccgcaag cgccggtcgc ggcgcccgcc gatcacgccg actccggcat
     1981 cgcctggaag caggcgtcca cggacgccga tgtcgacgcc gcgttcgcgc aggccaaggc
     2041 gcagaccaag ccggtgttcg tctactgggg cgcgaaatgg tgcccgccgt gcaaccaggt
     2101 caaggcgacc ttgttcaacc gccgggactt catcgagcgc tcgcgcgcct tcgtgccggt
     2161 ctacgtcgac ggcgacagcc cgggcgcgca gaagcttggg agccgcttca aggtcagcgg
     2221 ctacccgacg atgctgctgt tcaacccgca gggtgtcgag atgacgcggc tgcccggcga
     2281 ggtcgaggcc gagcgctaca cgcaggtgct gaccttgggg atgaacgccc agcgcccggt
     2341 cagggacctg ctcgcgcaag tcaaggccgg cggcgccggg ctcacggcca acgactggcg
     2401 cctgctcgcg ttctactcgt gggacaccga ccagcagcag ctcgtcacca aggacggtgt
     2461 gcccgcgctg atgaagcagc tggccgacgc ctgcccggcc gatcagaccg agatcgcgac
     2521 ccggctgcgc ctgaaggcgc tcgccgcggt cgacgacaag gcgccggccc gccccgacgc
     2581 cgcggcgcag gccacgctgc tgaaggtgct cgccgacgcg gcgagcgcgc gcccttacat
     2641 ggacgtgctg accaactacg cgaccgacat cacgcgcgcg ctcagcgcaa ggggttcgcc
     2701 gcagcgcgcc gagttgctgg ccgcgttcga cgcggcgctg aagcgcttcg aggccgacga
     2761 gaccctgtcg cgcgccgacc gcatgacggc gctgatcgcc caggtcgacc tggcgaccat
     2821 cgacgcgccg cgcggcgatg gcgcgaaggc ggccaagccc aagctgagcg acgcactgct
     2881 cgccgacatc cgagcgcaga ccacgcgtgc cgaccgcgag atcaccgacg gctacgagcg
     2941 ccagtcggtg atcaccgcgg ccgcctacct gctcgagcgt gccgggctgg gcggcgagtc
     3001 ggacgcgctg ctgcaggcca acctcgcgaa gagccactcg ccgtactacc tgatgagcga
     3061 gctcgcgagc aacgccaagg cgcgcggcga caagaccgag gcgctgcgct ggtacgagca
     3121 ggccttcgac aagagcgaag gcccggccac gcggctgcaa tggggcgcga gctacgtcag
     3181 cgcgctggtc gagatggcgc cgcacgacga cgcgcgcatc gagaaggccg cggcgcagct
     3241 gctgaccgag gcggccaggc agcccgacgc gttctacgag cgcagcggcc gcgcgctgca
     3301 gcgcgtcggc gcgaagctgc agcgctggaa caagggcggc gcacacgccg ccacgctgaa
     3361 gcggctgcag gcgcagctcg acggcgtctg cggcgcgctc gacgcgagcg acccgaaacg
     3421 cgcgaactgc gcggggctgt tcaagcccgc gcccaagcag gccgcgtgat cggcccgcgc
     3481 cggagcgggg taaatcagag ccgatcggca cggcgcttgc cttagcatgc gcgccatgcc
     3541 gaagccgcct gcagcgtccg acgattcgaa cttcagcgca gcgcgccgcc gggcgctgtc
     3601 gctggcggcg gcggcgctgg gcgcacgcgc gctgccgggc ttcgcggcag cgcgcggcga
     3661 cgacgccacc ggcgaaaccg gcccggtcgt cggcgtctgg acccacgcct acgcggcgtt
     3721 cggccgcccc aagtacccgc gcgactacgc caacttcgag tacgtcaacc ccgccgcgcc
     3781 aaagggcggc acgctgtacc tgcgcaaccc cgatcggcgc agcagc
//
LOCUS       DATMJB010000097         3898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_875087, whole genome shotgun sequence.
ACCESSION   DATMJB010000097 DATMJB010000000
VERSION     DATMJB010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875087"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VNU48_02145"
     CDS             <1..894
                     /locus_tag="VNU48_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HWI10109.1"
                     /translation="DVKIYEVRAADGSAVGVFLHDSFARATKRSGAWMSSFRRQSRNG
                     GAAGAERALPIVINNNNFAKGAPGEPTLLSFDDASTLFHEFGHGLHGLLSDVTYESVS
                     GTSVLRDFVELPSQIFEHWLAEPEVLKRHARHYRTDEPIPDALIEKLHDARLFNQGYE
                     TVRYTASALVDMAAHALTDAAAPDVVEFERAELARIAAPAVVGMNHRLTHFQHLFSGS
                     GYAAGYYVYLWAEVLDADGYDAFREAGNPFDPAVARRLREFIYATGNSIEPAAAYRAF
                     RGRGPTVEPMLKQRGLVPQGQ"
     gene            complement(860..>3898)
                     /locus_tag="VNU48_02150"
     CDS             complement(860..>3898)
                     /locus_tag="VNU48_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWI10110.1"
                     /translation="AAWLAQSHDLLALTDAAGRIQWCNPAFERSSGIGVAAELASLAP
                     PDWRAGVARRALTAALRSPPAQALELELRAASGAALWVDARVTALAEQRLWTLRDTSA
                     GHELAARAQHLSELLDIAQEFGRLGVWEREIPSGRGRWDRHVFGFWGMEPSDETPDYA
                     RAASHIHPEDRSSVYVESTQRAGRYAQRYRVLQPDGSIRRIHSQWEVKNSPEGMPDRT
                     VGVMVDDTEVYELARSLDSATTQLRIAAELADIVLWRHDLKTGRVHYNRHGFKVLGIP
                     YRSDGLPIDEARAYTHPDDRPKLEASAAQALQTDGPVDAQTRHRWPDGSWRHMLVRRV
                     VERDAAGQALAFVGVSLDLTEQAERSRRAEQLSQRLEAAARAARVGIWVTQVGAMHTE
                     WNAQMFELFDMVGAPRAPTLDEWIARCIHPDDRARVGHATRGYVRAARGALEIEFRSV
                     RRDATVRWIVLRADIERSEPEALRVFGIAVDVTERHDAQAALHAISERAALIARHAGI
                     GTWERGAGGPVLWDEQMFRLRGLEPRPVAPNRAERLALTHPDDRAFVLDGATDVDAGT
                     TPTAYEFRVRLPDGSYRWLASRSATLRDADGKVLRRVGVNWDVTEAKNAELARQQALL
                     AERESQAKSQFLSRMSHELRTPLNAVLGFTQLLQLEAQQLPPNGARAERLEHIRTAGE
                     HLLTLIDDVLDLSSLQAGMLALEPQDVTIAAAVGQSLPLLAELAGRQRVVVHRGALDG
                     TVRADPTRLRQVLLNLLTNAIKYNRAGGQVIVDAAVEAGLVRLRVRDTGRGLRPDQID
                     QLFEPFNRLGAEADGIAGSGIGLTIVKALVEGMGGRVDVSSVPGRGSVFEVTLPAVPV
                     AEVAARAAPPVRATAAKARSGQLLYIEDNSVNVLLVEELVKSLSGLRIASEATGAAGV
                     ARARVLQPDLVLVDMQLPDFDGFEVLRQMRTHPETVGIRCIALSANAMPDDIERGLAA
                     GFDDYWTKPIKFKPFLDALDRLFPASADHITDPAAPGRAA"
BASE COUNT          525 a         1414 c         1405 g          554 t
ORIGIN      
        1 gacgtgaaga tctacgaggt gcgcgcggcc gacggctcgg ccgtcggcgt gttcctgcac
       61 gacagcttcg cgcgcgccac caagcgcagc ggcgcatgga tgagctcgtt ccgccgccag
      121 tcgcgcaatg gcggcgccgc cggcgccgag cgcgcgctgc cgatcgtgat caacaacaac
      181 aacttcgcga agggcgcgcc cggcgagccg acgctgctga gcttcgacga cgcgagcacg
      241 ctgttccacg agttcggtca cggcctgcac gggctgctct cggacgtgac ctacgagagc
      301 gtctcgggca ccagcgtgct gcgcgacttc gtcgagctgc cgtcgcagat cttcgagcac
      361 tggctggccg agcccgaggt gctgaagcgc catgcgcgcc actaccggac cgacgagccg
      421 atccccgacg cgctgatcga gaagctgcac gacgcgcgcc tgttcaacca gggctacgag
      481 acggtgcgct acaccgcgtc ggcgctggtc gacatggccg cgcatgcgct gaccgacgcg
      541 gccgcgcccg acgtggtgga gttcgagcgc gccgagctgg cgcgcatcgc cgcgccggcg
      601 gtggtcggca tgaaccaccg gctgacgcac ttccagcacc tgttctcggg ctcgggctac
      661 gccgccggct actacgtcta cctgtgggcc gaggtgctcg acgccgacgg ctacgacgcg
      721 ttccgcgagg ccggcaaccc gttcgacccg gccgtggcgc ggcgcctgcg cgagttcatc
      781 tacgcgaccg gcaactcgat cgagccggcc gccgcgtacc gcgcgtttcg cggccgcggg
      841 ccgacggtcg agccgatgct caagcagcgc ggcctggtgc cgcagggtca gtgatgtgat
      901 ccgctgaggc cgggaacagg cggtcgagcg cgtccaggaa cggcttgaac ttgatcggct
      961 tggtccagta gtcgtcgaag cccgcggcca ggccgcgctc gatgtcgtcc ggcatcgcgt
     1021 tggccgacag cgcgatgcac cggatgccca ccgtctcggg gtgcgtgcgc atctggcgca
     1081 gcacctcgaa gccgtcgaaa tcgggcagct gcatgtcgac caggaccagg tccggctgca
     1141 gcacgcgggc gcgcgcgacg ccggccgcgc cggtcgcctc ggaggcgatg cgcaggcccg
     1201 acaggctctt cacgagttcc tcgaccagca gcacgttgac gctgttgtcc tcgatgtaga
     1261 gcagctgccc ggagcgcgcc ttcgccgccg tggcgcgcac gggcggcgcc gcgcgcgccg
     1321 cgacctcggc caccggcacc gcgggcagcg tgacctcgaa cacgctgccg cgcccgggca
     1381 cgctggacac gtcgacccgc ccgcccatgc cctcgacgag tgccttgacg atcgtcaggc
     1441 cgatgcccga gcctgcgatg ccgtcggcct cggcgccgag ccggttgaag ggttcgaaca
     1501 actgatcgat ctgatcgggc cgcaggccgc ggccggtgtc gcgcacgcgc agccgcacga
     1561 ggccggcttc gacggcggcg tcgacgatca cctggccgcc cgcccggttg tacttgatcg
     1621 cgttcgtcag caggttcagc agcacctggc gcagccgggt cgggtcggcg cgcaccgtgc
     1681 cgtcgagtgc gccgcggtgc acgaccacgc gctgcctgcc cgcgagctcg gccaacagcg
     1741 gcagcgactg tccgacggcg gcggcgatgg tcacgtcctg cggctcgagc gcgagcatgc
     1801 cggcctgcag gctcgacagg tcgagcacgt cgtcgatcag cgtcagcagg tgctcgcccg
     1861 cggtgcggat gtgctcgagc cgctcggccc gggcgccgtt gggcggcagc tgctgcgcct
     1921 cgagctgcag cagttgcgtg aagccgagca ccgcgttcag cggcgtgcgc agttcatggc
     1981 tcatgcgcga caggaactgc gacttggcct ggctctcgcg ctccgccagc agcgcctgct
     2041 ggcgggcgag ctcggcgttc ttcgcttcgg tgacatccca gttcacgccg acgcgccgga
     2101 gcaccttgcc gtcggcatcg cgcagcgtcg ccgaacgcga ggccagccag cgatagctgc
     2161 cgtcgggcag gcgcacgcgg aactcgtagg ccgtcggcgt ggtgcccgcg tcgacgtccg
     2221 tggcgccgtc gaggacgaat gcgcgatcgt cgggatgcgt gagcgccagg cgctcggcgc
     2281 ggttgggcgc gacggggcgc ggttcgaggc cgcgcagccg gaacatctgc tcgtcccaca
     2341 gcaccggccc cccggcgccg cgttcccagg tgccgatgcc ggcatggcgc gcgatcagcg
     2401 cggcccgttc gctgatcgca tggagcgccg cctgtgcgtc gtgccgctcg gtcacgtcca
     2461 ccgcgatgcc gaacacgcgc agcgcttcgg gctcgctgcg ttcgatgtcg gcacgcagga
     2521 cgatccagcg caccgtcgcg tcgcgcctca cggaacggaa ctcgatctcg agcgcgccgc
     2581 gcgccgcgcg cacgtacccg cgggtggcgt ggccgacgcg cgcccggtcg tcggggtgga
     2641 tgcagcgggc gatccattcg tccagcgtcg gcgcacgcgg cgccccgacc atgtcgaaca
     2701 gctcgaacat ctgcgcgttc cattcggtgt gcatcgcgcc gacctgagtc acccagatgc
     2761 ccacccgcgc ggcacgcgcc gccgcttcga gacgctgcga gagctgctcg gcgcgacgcg
     2821 agcgctcggc ctgttcggtc aggtcgagcg agacgccgac gaaggccagc gcctggcccg
     2881 ccgcgtcgcg ctcgacgacc cggcgcacca gcatgtgccg ccagctgcca tcgggccagc
     2941 ggtggcgggt ctgcgcgtcc accggcccgt cggtctgcag cgcctgcgcc gccgacgcct
     3001 cgagcttcgg gcggtcgtcc gggtgggtgt aggcccgcgc ctcgtcgatc ggcaggccgt
     3061 cgctgcggta ggggatgccc agcaccttga agccgtgccg gttgtagtgc acgcggccgg
     3121 tcttcaggtc gtggcgccag agcacgatgt cggcgagttc ggcggcgatg cgcaactgcg
     3181 tcgtggcgct gtcgagcgag cgcgcgagtt cgtagacctc ggtgtcgtcc accatcacgc
     3241 cgacggtgcg gtcgggcatg ccctcgggcg aattcttcac ctcccattgc gaatggatgc
     3301 gccgaatgct gccgtcgggc tgcagcacgc ggtagcgctg tgcgtagcgg ccggcgcgct
     3361 gcgtcgattc gacgtagacc gaggaccggt cctcggggtg gatgtgcgag gccgcgcgtg
     3421 cgtagtccgg cgtctcgtcg gaaggctcca tgccccagaa gccgaacaca tgccggtccc
     3481 agcgaccccg gccggacggg atctcccgct cccacacgcc caggcgaccg aactcctgcg
     3541 cgatgtcgag caactcggac aggtgctgcg cgcgcgccgc gagttcgtgg ccggcgctgg
     3601 tgtcgcgcaa ggtccacagg cgctgctcag ccagggccgt gacgcgcgca tcgacccaca
     3661 gtgcggcgcc cgatgcggcg cgcaactcga gttcgagcgc ctgcgctggc ggcgatcgaa
     3721 gcgccgcggt cagcgcgcgc cgcgcaacgc cggcgcgcca gtcgggcggc gccaggctcg
     3781 cgagctcggc ggccacgccg atgccgctgc tgcgctcgaa cgccgggttg caccactgga
     3841 tgcgtcccgc cgcgtcggtc agcgcaagca ggtcgtggct ctgtgccagc cacgccgc
//
LOCUS       DATMJB010000098         6354 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_282880, whole genome shotgun sequence.
ACCESSION   DATMJB010000098 DATMJB010000000
VERSION     DATMJB010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282880"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VNU48_02155"
     CDS             complement(<1..643)
                     /locus_tag="VNU48_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase kinase/phosphatase AceK
                     regulatory subunit"
                     /protein_id="HWI10111.1"
                     /translation="MRAASFPTALTDPRAFDIAQAMLDGFNRHYRLFREASADAKRRF
                     EEADWHGQQRAQRERIEFYDKRVDEAAERLQTQFEAAELPKDVWQQVKLHYIGLLIDH
                     HQPELAETFFNSVTTKILHRRYFNNDFIFVRPAVSTEYIENDEPASEPTYRAYYPTKA
                     TMRETFLRIVTNFQLEPEFEDLGRDIDQVLAAVIARLGEFRMRANFQVQVLSSL"
     gene            complement(643..1314)
                     /gene="can"
                     /locus_tag="VNU48_02160"
     CDS             complement(643..1314)
                     /gene="can"
                     /locus_tag="VNU48_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HWI10112.1"
                     /translation="MTTQLQELLDSNRLWAAKTESRSPGFFTRLLTQQAPQYLWIGCA
                     DSRVPANELVDLLPGELFVHRNVANVVVHSDLNCLSVIQFATDLLKVKHIIVVGHSRC
                     AGVVAALDGTRVGLADNWIRHVQDVRNRHQEWLAGLPAEQRIDALGELNVIEQARNVC
                     QTTVVEDAWARGQELVVHGWFYGLNNGLLRDLQITVPNAAAMAAAFDDAIAALHSRHA
                     LLTTP"
     gene            complement(1408..2205)
                     /locus_tag="VNU48_02165"
     CDS             complement(1408..2205)
                     /locus_tag="VNU48_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10113.1"
                     /translation="MPTRRVLVLALAAVAAAGAAACSAPAFATGGLVDVEIVDRGRNE
                     VLPTYRQRGTSWVAGRPGSRYAVRLRNRCGNRVLVVLSVDGVNAISGETASASQTGYV
                     LAPYQSAEITGWRKSMSEAAAFYFTALPDSYAARTGRPDNVGVIGVAVFRERERERVV
                     EVAPRAFDRAAQAQSATRERGDAAPAAPAAKAEGQLRDERLGTGHGEREYAPITHTAF
                     ERASDRPDEIVQLRYDSVENLIALGVLRPTRVPPPEPFPGFVPDPKG"
     gene            2376..2597
                     /locus_tag="VNU48_02170"
     CDS             2376..2597
                     /locus_tag="VNU48_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018915417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWI10114.1"
                     /translation="MSESTVTAKGQTTVPAQVRERVGATPGTRLVWHVMPDGSIIVRA
                     KTKSILDLAGSLRAPKGKRVAIEDMNAWR"
     gene            2597..2998
                     /locus_tag="VNU48_02175"
     CDS             2597..2998
                     /locus_tag="VNU48_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWI10115.1"
                     /translation="MAALDTNVLVRFLVRDDAAQLAAAKRLIRRCIAEGQTLFVPLTV
                     SLELEWVLRSSFGFGKDEVIRALSQLLSAAELSFESEAALEFALSRYQEGAADYADCL
                     HLALAVQAGAQPLWTFDRAASKLAGARLLGA"
     gene            complement(3017..4198)
                     /locus_tag="VNU48_02180"
     CDS             complement(3017..4198)
                     /locus_tag="VNU48_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HWI10116.1"
                     /translation="MNNLPGLNYQLGEDVDALRDAVREFAQAEIAPRAAEIDASDQFP
                     MDLWRKMGSLGVLGITVSEEYGGAGMGYLAHMIAMEEISRASASVGLSYGAHSNLCVN
                     QIKRNGSDAQRARYLPKLISGEHVGALAMSEPGAGSDVISMKLKAQERGDHYLLNGSK
                     MWITNGPDADTLVVYAKTEPELGARGVTAFLIEKGMKGFSIAQKLDKLGMRGSHTGEL
                     VFDNVEVPAENVLGALNGGAKVLMSGLDYERAVLTGGPLGIMQSVMDNVMPYVHDRKQ
                     FGQSIGEFQLVQGKVADMYTVLQAGRAFCYTVGKNLDLLGAEHVRQVRKDCASVILWC
                     AEKATWMAGEGIQIFGGNGYINEYPLGRLWRDAKLYEIGAGTSEIRRMLIGRELFAET
                     L"
     gene            complement(4223..4438)
                     /locus_tag="VNU48_02185"
     CDS             complement(4223..4438)
                     /locus_tag="VNU48_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10117.1"
                     /translation="MPPTPVSNRASTALDSACGCAPRGRVKKAGRTISVVDGEAFEHE
                     AGSHERKRVATMTATIMTITGRDDVRH"
     gene            complement(4531..4926)
                     /locus_tag="VNU48_02190"
     CDS             complement(4531..4926)
                     /locus_tag="VNU48_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWI10118.1"
                     /translation="MNPEFFATWTPRALAALRIVTAFLFLQHGTAKLLHVPHVAMFDT
                     LPLLSLFGVAGVLEIAGSLLVLTGWFTRAAAFVLSGEMAVAYFVAHVPSGHLLSPMLN
                     DGEPAVLFCFVFLLLSVAGPGTWSVDKTA"
     gene            complement(4982..5374)
                     /locus_tag="VNU48_02195"
     CDS             complement(4982..5374)
                     /locus_tag="VNU48_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWI10119.1"
                     /translation="MSATFTIGELAREFDLTTRAIRFYEDCGLLAPQRSGRNRVYTHR
                     DRTRLKLTLRGKRLGLTLAEVRELVDMYESPRDTLPQLKKFLVVLAAHREQLEQQMAD
                     LQATLAEVRSHEREARRLLADGQKKASA"
     gene            5479..5598
                     /locus_tag="VNU48_02200"
                     /pseudo
     CDS             5479..5598
                     /locus_tag="VNU48_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576395.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            complement(5577..5777)
                     /locus_tag="VNU48_02205"
                     /pseudo
     CDS             complement(5577..5777)
                     /locus_tag="VNU48_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
     gene            complement(5699..5881)
                     /locus_tag="VNU48_02210"
     CDS             complement(5699..5881)
                     /locus_tag="VNU48_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10120.1"
                     /translation="MSADPTRVDIVSIGEPMIEFNQLRPGAPERSRWRARLAPAIASP
                     ARCWRGSRPATNWRPR"
     gene            complement(5926..>6354)
                     /locus_tag="VNU48_02215"
     CDS             complement(5926..>6354)
                     /locus_tag="VNU48_02215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HWI10121.1"
                     /translation="VLHTAEALPGVPRLTLHVGLPHTATPEQIRDAVQSWLQRQARRV
                     FEARCAHFAQQLKVRVTRLALSSAQTRWGSASADGSIRLNWRLIHFAMPSIDYVVAHE
                     LAHLREMNHSARFWDVVRSVLPDYERARGSLKDGLVPLLD"
BASE COUNT          902 a         2183 c         2242 g         1027 t
ORIGIN      
        1 acagcgacga cagcacctgc acctgaaagt tggcgcgcat tcggaactcg ccgaggcgcg
       61 cgatcaccgc ggcgagcacc tgatcgatgt cgcgccccag gtcctcgaac tcgggctcga
      121 gctggaagtt cgtgacgatg cgcaggaacg tttcgcgcat cgtcgccttc gtcgggtagt
      181 aggcgcggta ggtgggctcc gaggccggtt cgtcgttttc gatgtattcg gtcgagaccg
      241 ccggacggac gaagatgaag tcgttgttga aatagcgcct gtgcaggatc ttcgtcgtca
      301 ccgagttgaa gaaggtctcg gccaactcgg gctggtggtg gtcgatcaac aggccgatgt
      361 agtgcagctt cacttgctgc cagacgtcct tcggcagctc ggcagcctcg aactgcgtct
      421 gcagccgttc ggccgcttcg tcgacgcgct tgtcgtagaa ctcgatgcgc tcgcgctgtg
      481 cgcgctgctg gccgtgccag tcggcctctt cgaaacggcg cttcgcgtcg gcgctcgcct
      541 cgcggaacag ccggtagtgg cggttgaagc cgtcgagcat cgcctgcgcg atgtcgaacg
      601 cgcgcgggtc ggtcagcgcg gtcgggaagc tcgccgcccg catcacggcg tcgtcagcag
      661 cgcgtggcgc gagtgcagcg ccgcgatcgc gtcatcgaag gccgccgcca tggcggcggc
      721 gttgggcacg gtgatctgca gatcgcgcag caggccgttg ttcaggccgt agaaccagcc
      781 gtgcacgacc agctcctggc cgcgcgccca ggcgtcctcg accacggtgg tctggcagac
      841 attgcgcgcc tgctcgatca cgttgagctc gcccagcgcg tcgatgcgct gctcggccgg
      901 caggccggcc agccactcct ggtggcggtt gcgcacgtcc tgcacatggc ggatccagtt
      961 gtcggccagg ccgacgcgcg tgccgtctag cgccgccacc acgccggcgc agcgcgagtg
     1021 gccgacgacg atgatatgct tgaccttcag caggtcggtc gcgaactgga tcaccgacag
     1081 gcagttcagg tcggagtgca cgaccacgtt ggcgacgttg cggtgcacga acagctcgcc
     1141 cggcagcagg tcgaccagct cgttcgccgg cacccgcgag tcggcgcagc cgatccacag
     1201 gtactgcggc gcctgctgcg tcagcagcct ggtgaagaag ccggggctgc gcgactcggt
     1261 cttcgcggcc cacaggcggt tgctgtcgag aagctcttgc agctgggtgg tcatggtgca
     1321 gtgcggcaga attttcacaa gtgtacgcac cgcaccgacg cccggcctgc tcgcgccggg
     1381 cgccatcggg gccgaccagc cgcttcttca gcctttcggg tcgggcacga agcctgggaa
     1441 aggctcgggc ggcggcacgc gcgtcggtcg cagcacgccg agcgcgatca ggttctcgac
     1501 gctgtcgtag cgcagctgca cgatctcgtc gggccggtcg ctcgcgcgct cgaacgcggt
     1561 gtgcgtgatc ggcgcgtatt cgcgttcgcc gtggccggtg cccaggcgct cgtcacgcaa
     1621 ctggccttcg gccttcgcgg ccggcgcggc cggggccgca tcgccgcgct cgcgcgtcgc
     1681 gctctgggcc tgcgcggcgc ggtcgaacgc acgcggtgcg acctcgacga cgcgctcgcg
     1741 ctcgcgttcg cggaacaccg cgacgccgat cacgccgacg ttgtcgggcc ggccggtgcg
     1801 cgcggcatac gagtcgggca gcgcggtgaa gtagaacgcc gccgcctcgc tcatgctctt
     1861 gcgccagccg gtgatctcgg cgctctggta cggcgcgagc acgtagccgg tctggctcgc
     1921 gctcgcggtc tcgccgctga tcgcgttgac gccgtcgacc gacagcacca ccagcacgcg
     1981 gttgccgcag cggttgcgca ggcgcaccgc gtagcggctg ccggggcgcc cggcgaccca
     2041 gctcgtgccg cgctggcggt aggtcggcag cacctcgttg cggccgcggt cgacgatctc
     2101 gacgtcgacg aggccgccgg tggcgaacgc gggcgcgctg caggccgcgg cgccggcagc
     2161 ggcgacagcg gcgagcgcga ggacaaggac acggcgggtc ggcatgtgaa gtctccggtg
     2221 cggggtttcg acatgcggtt caacggcgca gcgcgcgtga cggggtgaag cgcccacaaa
     2281 ttattctttg cggccgccgc gtcgatcggt cggcgtgctc atcgccgggg ggcgcccgtg
     2341 gcgcattaca atgctggagt atgtaaggag tcacgatgag cgaatccacc gttaccgcca
     2401 agggacagac gaccgttccg gcccaggttc gcgagcgggt cggtgcgacg ccggggaccc
     2461 ggctggtgtg gcatgtgatg cccgacggca gcatcatcgt gcgcgccaag acgaaatcca
     2521 tcctggactt ggcggggtcg ttgcgggcgc cgaagggcaa gcgcgtcgcg atcgaagaca
     2581 tgaacgcctg gcgctgatgg cggcgctgga cacgaatgtg ctggtgcgct tcctcgtccg
     2641 cgacgatgcg gcgcagctcg ccgccgcgaa gcgcctgatc cgcaggtgca tcgccgaagg
     2701 gcagacgctg ttcgtaccgc tgacggtctc gctcgaactc gaatgggtgc tgcgctcgag
     2761 cttcggcttc ggcaaggacg aggtgatccg ggccctgtcg cagctgctgt cggccgcgga
     2821 gctgagtttc gagtcggaag ccgcactcga attcgcactg tcgcgctacc aggagggggc
     2881 ggccgactac gccgactgcc tgcatctggc gctggccgtc caggccggcg cgcaaccgct
     2941 gtggactttc gacagggcgg cttccaagct ggccggcgcc cggctgctgg gcgcgtaggg
     3001 cccgcccgcg cgtgcgctac agggtctcgg cgaacagctc gcggccgatc agcatgcgcc
     3061 ggatctcgct ggtgccggcg ccgatctcgt aaagcttcgc gtcgcgccac agccggccga
     3121 gcgggtactc gttgatgtag ccgttgccgc cgaagatctg gatgccctcg ccggccatcc
     3181 aggtcgcctt ctcggcgcac cacaggatca cgctcgcgca gtccttgcgc acctggcgca
     3241 cgtgctcggc gccgagcagg tcgaggttct tgcccaccgt gtagcagaac gcgcggcctg
     3301 cctgcagcac cgtgtacatg tcggccacct tgccctgcac gagctggaac tcgccgatgc
     3361 tctggccgaa ctgcttgcgg tcgtggacgt acggcatgac gttgtccatc accgactgca
     3421 tgatgccgag cggcccgccg gtcagcaccg cgcgctcgta gtccagcccg ctcatcagca
     3481 ccttcgcgcc gccgttcagc gcaccgagca cgttctcggc gggcacctcg acattgtcga
     3541 agacgagctc gccggtgtgg ctgccgcgca tgccgagctt gtcgagcttc tgcgcgatgg
     3601 agaagccctt catgcccttc tcgatcagga acgcggtgac gccgcgcgcg ccgagctcgg
     3661 gctcggtctt cgcgtacacg accagggtgt cggcgtcggg gccgttggtg atccacatct
     3721 tgctgccgtt gagcaggtag tggtcgccgc gctcctgcgc cttcagcttc atgctgatca
     3781 cgtccgagcc ggcgcccggc tcgctcatcg cgagcgcgcc gacatgctcg ccgctgatca
     3841 gcttcggcag gtacctggcg cgctgcgcgt cgctgccgtt gcgcttgatc tggttcacgc
     3901 acaggttgct gtgcgcgccg tagctcaggc ccaccgacgc gctggcgcgc gagatctctt
     3961 ccatcgcgat catgtgcgcg aggtagccca tgccggcgcc accgtactcc tcgctcaccg
     4021 tgatgccgag cacgccgagc gatcccatct tgcgccacag gtccatcggg aactggtcgc
     4081 tggcgtcgat ctcggcggcg cgcggcgcga tctcggcctg cgcaaattcg cgcaccgcgt
     4141 cgcgcagcgc gtcgacgtct tcgccgagct ggtagttcag gccgggcagg ttattcatga
     4201 gcttgtctcc ttgctcgtcg gtctagtggc gcacgtcgtc gcggccggtg atcgtcatga
     4261 tggtggcggt catcgtcgcc acgcgtttgc gctcgtggct gccggcctcg tgctcgaagg
     4321 cctcgccgtc gacgacgctg atcgtgcgcc cggccttctt gacccgcccc ctcggcgcgc
     4381 agccgcaggc cgagtcgagc gcggtggatg cgcggttcga aaccggggtc ggcggcattc
     4441 atgggcgttc ggcgcgtggg caaccgcagt tgacgtatac gtcaattgta gcccgcgctg
     4501 gggccgccgc cgcgccgagc gcgccgggcc tcaggcggtc ttgtccacgc tccaggtgcc
     4561 ggggccggcc accgacagca gcaggaacac gaagcagaac agcaccgccg gctcgccgtc
     4621 gttcagcatc ggcgacagca aatggccgct cggcacgtgg gccacgaagt acgccaccgc
     4681 catctcgccc gacaggacga aggccgccgc ccgcgtgaac cagccggtca gcaccagcag
     4741 gctgccggcg atctcgagca cgccggcgac gccgaacagc gacagcagcg gcagcgtgtc
     4801 gaacatcgcc acatgcggca cgtgcagcag cttggcggtg ccgtgctgca ggaacaggaa
     4861 cgcggtgacg atgcgcagcg cggcgagcgc gcgcggggtc caggtggcga agaattccgg
     4921 gttcatgcga tgtctccaga agccagacgt gggggcccga ttctggaccg ctgggccacc
     4981 gctaggccga cgccttcttc tgcccgtcgg cgagcaggcg ccgcgcctcg cgctcgtggc
     5041 tgcgcacctc ggccagcgtc gcctgcaggt cggccatctg ctgctcgagc tgctcgcggt
     5101 gcgccgccag cacgaccagg aacttcttca gctgcggcag cgtgtcgcgc ggcgattcgt
     5161 acatgtccac cagttcgcgc acctcggcca gcgtcagccc gagccgcttg ccgcgcaacg
     5221 tgagcttcag ccgggtgcgg tcgcgatggg tgtagacgcg gttgcggccg ctgcgctgcg
     5281 gcgcgagcag gccgcagtct tcgtagaagc ggatcgcgcg ggtcgtgagg tcgaactcgc
     5341 gggcgagctc gccgatcgtg aacgtggcag acatatgggt gacagcggcg gcgcgacgag
     5401 cgctctacac tggctcgatt gacgtttacg taaagttcgt aaagtgtagt tgggaacccc
     5461 ctcgatggcg atgaccacct gcaccagacg accttcgcga tgggcgagtc ggtcgcgcac
     5521 ctgaatgccc tgtggctcgc gggccggctg acgcgccggc tcgacgccga cggcatctac
     5581 cgcttcgccg ccgtctgatc gccggcggcc agcagcgcgc gcaccgccgc cgggtgcggg
     5641 atcggcgcga ccgcgccgaa gccggtggtc gtcagcgcgg ccgccgcgtt ggcgtaggtc
     5701 accgcggcct ccagttcgtc gccggccgcg agccgcgcca gcagcgcgcc ggcgaagcaa
     5761 tcgccggcgc cagtcgcgcg cgccagcgcg atcgctctgg cgcgccgggg cgcagctggt
     5821 tgaactcgat catcggctcg ccgatcgaga cgatgtccac gcgggtcggg tcggcgctca
     5881 tgcgaaggcg ttcgactcgg tgcgacgcgc gcggggcgcc cgggctcagt ccagcaacgg
     5941 caccagccca tccttcaggc tgccgcgcgc acgctcgtag tcgggcagca ccgagcgcac
     6001 cacgtcccag aagcgcgcgc tgtggttcat ctcgcgcagg tgtgcgagtt cgtgcgcgac
     6061 gacgtagtcg atgctcggca tcgcgaaatg gatcagccgc cagttcagcc ggatcgagcc
     6121 atcggcgctg gcgctgcccc agcgcgtctg cgccgaggac agcgccagcc gcgtcacgcg
     6181 caccttcagc tgctgcgcga agtgcgcgca gcgcgcctcg aagacgcgcc gcgcctggcg
     6241 ctgcagccag ctctgcacgg cgtcgcggat ctgctccggc gtcgcggtgt gcggcaagcc
     6301 cacgtgcagc gtgagccgcg gcacgccggg caaggcttcg gccgtgtgca gcac
//
LOCUS       DATMJB010000099         2153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_815383, whole genome shotgun sequence.
ACCESSION   DATMJB010000099 DATMJB010000000
VERSION     DATMJB010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815383"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VNU48_02220"
     CDS             complement(<1..303)
                     /locus_tag="VNU48_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI10122.1"
                     /translation="MSMPAALLRLDRVGVCYGAVTALREVSLQVRRGDFVALVGANGS
                     GKTTLLQALHGLVAHSGTREVAAAAAAQVMVFQRPFVLRLSVWNNLSIALWLAHVPA"
     gene            complement(300..1013)
                     /locus_tag="VNU48_02225"
     CDS             complement(300..1013)
                     /locus_tag="VNU48_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI10123.1"
                     /translation="MSAFAESFRTAVTLVLEADPVLWRTVRLSLAVSLSACAIAAGIG
                     LATGAWLAVAQFPGRRALLALLNTLLALPSVVVGLVVYLLLSRSGPLGDWGILFTPGA
                     MVIAQTILVLPVIAALSRQLVADSLRDGGDQLRSMGARPFTAALLMLLHERWAVATVL
                     LTAFGRAVSEVGAVMIVGGNIDGVTRVITTTIALETSKGDLPLALGLGLVLLALVGVV
                     NAGIALAQRAQGLGLAAAR"
     gene            1077..>2153
                     /locus_tag="VNU48_02230"
     CDS             1077..>2153
                     /locus_tag="VNU48_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWI10124.1"
                     /translation="MHRVQLAYTLADERAADRALHHPLLALLAALHATGSISAAARQL
                     GLSYRHVWGELKRWETELGRPLVRWAKGQPAALAPFGEKLLWAERRALARLAPQIEAL
                     RSELERAFALAFDDSAGVVTLFASFDEALPRLRDRVAPRLHLDIEFTGSVDALAALND
                     GRCLLAGFHALTAAKLRSPTARTYRGMLRPGRHKLIGFARRAQGLIVAAGNPLAIGAI
                     DDLARPGLRFANGPRGTGTRVVLEELLAAARLAPAAIAGFDRFERSHQAAAEAVASGA
                     ADAAFGTLPAAQAKGLHFVPLAQEQYFLVTLAAHLTHPHVLTLLQELQAPRWRDALDA
                     IPGHAAQRCGEVLSLRQVLPWWNYR"
BASE COUNT          307 a          814 c          776 g          256 t
ORIGIN      
        1 cgccggcacg tgcgccagcc acagcgcgat gctcaggttg ttccagaccg acaggcgcag
       61 cacgaacggg cgctggaaca ccatcacctg cgccgccgcg gccgccgcga cctcgcgcgt
      121 gcccgagtgc gcgaccaggc cgtgcagcgc ctgcagcagc gtggtcttgc ccgagccgtt
      181 ggcgccgacc agcgcgacga aatcgccgcg acgcacctgc aggctgacct cgcgcagcgc
      241 ggtcaccgcg ccgtagcaca cgccgacgcg gtccagcctc agcagcgcgg ccggcatgct
      301 catcgcgccg cggccaggcc gaggccctgc gcgcgctgcg cgagcgcgat gccggcgttg
      361 acgacgccga ccagcgcgag cagcacgagg ccgaggccga gcgcaagcgg caggtcgccc
      421 ttgctggtct cgagcgcgat cgtcgtcgtg atgacgcgcg tgacgccgtc gatgttgccg
      481 ccgacgatca tcaccgcgcc gacctcggag acggcgcgcc cgaaggcggt cagcagcacg
      541 gtggccaccg cccagcgctc gtgcagcagc atcagcagcg ccgcggtgaa cgggcgcgcg
      601 cccatcgaac gcagctggtc gccgccgtcg cgcaggctgt cggcgaccag ctggcgcgac
      661 aaggcggcga tcaccggcag caccaggatg gtctgcgcga tcaccatcgc gccgggcgtg
      721 aacaggatgc cccagtcgcc cagcggcccg ctgcgcgaca gcagcaggta gacgaccaga
      781 ccgaccacca ccgagggcag cgcgagcagc gtgttcagca gcgcgagcag cgcgcgccgg
      841 cccgggaact gcgcgaccgc gagccacgcg ccggtggcca ggccgatgcc cgcggcgatc
      901 gcgcaggccg agaggctgac cgcgagcgac aggcgcaccg tgcgccacag caccgggtcg
      961 gcctcgagca cgagcgtgac ggcggtgcga aaactctcgg cgaaggcgct caacgaactt
     1021 ctctcatact ggacgattct ccacggcgcg cgcaagccca cgcgatatga acttccatgc
     1081 atagggtgca gctggcctac acgctcgcgg acgagcgcgc cgcggatcgg gccttgcacc
     1141 atccgctgct ggcgctgctc gcggcgctgc acgcgaccgg ctcgatctcg gccgcggcgc
     1201 gccagctcgg gctgtcgtac cgccatgtct ggggcgaact gaaacgctgg gagaccgagc
     1261 tcggccggcc gctggtgcgc tgggcgaagg gccagccggc cgcgctcgcg ccgttcggcg
     1321 agaagcttct gtgggccgag cgtcgcgcgc tggcgcggct ggcgccgcag atcgaggcgc
     1381 tgcgcagcga actcgaacgc gcgttcgcgc tcgccttcga cgacagcgcc ggcgtggtga
     1441 ccctgttcgc gagcttcgac gaagcgctgc cgcggctgcg cgaccgggtc gcgccgcgcc
     1501 tgcacctgga catcgagttc accggcagcg tcgatgcgct ggccgcgctg aacgatgggc
     1561 gctgcctgct cgccggcttt catgcgctga cggccgccaa gctgcgctcg cccaccgcgc
     1621 gcacctaccg cggcatgctg cggcccggcc gccacaagct gatcggcttt gcgcggcgcg
     1681 cgcagggcct gatcgtcgcg gccggcaacc cgctcgcgat cggggcgatc gacgacctcg
     1741 cgcggcccgg gctgcgcttc gcgaacggcc cgcgcggcac cggaacgcgc gtcgtgctcg
     1801 aggagctgct ggccgcggcc cggctggcgc ccgccgccat cgctggcttc gaccgcttcg
     1861 agcgctcgca tcaggcggcg gccgaagcgg tcgcgagcgg cgccgccgac gcggccttcg
     1921 gcacgctgcc ggcggcgcag gccaagggcc tgcatttcgt gccgctggcg caggagcagt
     1981 acttcctcgt cacgctggcc gcgcacctga cgcacccaca cgtgctcacg ctgctgcagg
     2041 aactgcaggc gccgcgctgg cgcgacgcgc tcgacgcgat ccccggccac gcggcgcagc
     2101 gctgcggcga ggtgctgtcg ctgcgccagg tcttgccgtg gtggaactac cgc
//
LOCUS       DATMJB010000100         3194 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_696483, whole genome shotgun sequence.
ACCESSION   DATMJB010000100 DATMJB010000000
VERSION     DATMJB010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696483"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VNU48_02235"
     CDS             complement(<1..1194)
                     /locus_tag="VNU48_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10125.1"
                     /translation="MKPLFPHRGRPRLGGVAAGVWLLAFVAIAVLQLAADSLTLKSYD
                     EGLILYGAARVAHGAVPYRDFWSMYGPGSFYALAGLDRLFGESVLLGRCFDAAARAAI
                     VVLVFALTWRRSGRFAAGAAAVVAFLLLVGVRDYLFPALPATAFALLAMLALDRALAG
                     SAAAQVDAPGAARAWALPGAAVGMTLLFRHDFGVCTALACLWPLLTREQAGLGRRFAA
                     FAVGTLVTAGPLMVALLVQVPLRDLYDNLLRIPLQVYVANRELPFPSVAQALRDAIER
                     HAVAPVLPLATYLPLLVAAVSVPMVLYRRFAGAPPPRDQWPPRALFDALVLLNALLCL
                     KGAVRVEVLQMLPAMMVSIVLVARAVARPGARGVGAPGAALLLVAFGGVAGAVVAGAV
                     VARAVA"
     gene            complement(1306..2028)
                     /locus_tag="VNU48_02240"
     CDS             complement(1306..2028)
                     /locus_tag="VNU48_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10126.1"
                     /translation="MSEFERVRAETGGSGFARLGHATVLPTTLLADLRRLGGGVAGAE
                     PLEVVAACVRHHEPALLCLQYEDLVWPVTLFPAQMLYHSPRDMALASRASLMRLALLS
                     TEPPGVRPPGHWMHERIAAAQHYRPLQPLLWSLALDGPRSTLLAEIAGTAAYRALRSV
                     EVDGVSPLAGALHSAAQRLRRETVSLREISSWPGMSVERASRLLNGLYLASSLLATRA
                     HPAARAEPRGLRALWRATKPRR"
     gene            2258..>3194
                     /locus_tag="VNU48_02245"
     CDS             2258..>3194
                     /locus_tag="VNU48_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10127.1"
                     /translation="MNDRQTPSFAAVGGLADATLPAGTRLGSFELQRAIARSPSSVVY
                     QATDHALALTVAIQEYLPARLVRRDSGLRLRATDTWHEDVIARGLRAFIDEARLLAHC
                     DHPALVRVNQLFEVNGSAYRVMPFYSGRRLLDLRRAMPEAPDEASLRALLDDLLGALE
                     AIHRGGHAHGGVTPGNILLLGDDRPLLLGPGAAAREIGSDLVDSLMASLETPLAPEPA
                     EVTPTGPIADLYALAEVVRFCITGTPTPVAVPGGSGRESMAEVIAHSFEPAARPRYSA
                     ELLGTLDAALSPLAEDWPLSVAQLRTWLARGAPRAA"
BASE COUNT          492 a         1217 c         1135 g          350 t
ORIGIN      
        1 cgcgacggcg cgcgcgacga ccgcaccggc gacgaccgca ccggcgacgc cgccgaacgc
       61 gacgagaagc agcgccgcac ccggcgcacc cacgccgcgc gcccccgggc gcgccacggc
      121 gcgggccacc agcacgatcg agaccatcat cgccggcagc atctggagca cctcgacgcg
      181 aaccgcgccc ttcaggcaca gcagcgcgtt gagcaggacc agcgcgtcga acagggcgcg
      241 cggcggccat tggtcgcgag gcggcggcgc gcccgcgaag cgccgataca agaccatcgg
      301 cacactcacc gcggcgacca gcagcggcag ataggtcgcc aacggcagca cgggcgcgac
      361 cgcatggcgc tctatcgcgt cgcgcagcgc ctgggccacg gacggaaagg gcagctcgcg
      421 attcgccacg tagacctgca gcggaatccg cagcaggttg tcgtagaggt cacgcagcgg
      481 cacctgcacg agcagcgcaa ccatcagcgg cccggcggtc acgagcgtgc cgacggcgaa
      541 cgcagcgaag cggcgcccga gccccgcttg ctctcgcgtc agcagcggcc acaggcaggc
      601 aagcgcggtg cagacaccga agtcgtgccg gaacagcagc gtcatcccga ccgcggcgcc
      661 cggcagcgcc cacgcgcgcg ccgctcccgg ggcatcgacc tgcgccgccg cggaacctgc
      721 cagcgcgcga tccagcgcga gcatcgccag cagtgcgaac gcggtggcgg gcagcgccgg
      781 aaacaggtag tcgcgcacgc cgaccagcaa caggaaggca acgaccgccg cggcgcccgc
      841 ggcgaaccgg ccggagcggc gccaggtcag cgcgaacacc agcacgacga tcgcggcacg
      901 ggccgccgca tcgaagcaac gcccgagcag caccgactcg ccgaagagcc ggtccaggcc
      961 ggcgagcgcg tagaagctgc cgggcccgta catcgaccag aagtcgcggt acggcacggc
     1021 gccatgggcg acgcgcgccg cgccgtaaag gatcaagccc tcgtcgtacg acttcagcgt
     1081 caggctgtcg gccgcgagtt gcagcacggc gatggccacg aaggcgagca gccagacgcc
     1141 ggccgcgacc ccgccgagcc gcggccggcc gcgatgagga aacaaaggtt tcatgggatg
     1201 ggcgcagagc gtagcagccc gctgtggctc gctgggtacc gcgcatctcg gcctgtccgg
     1261 gccgtggact tacaccatgc gctgggacac gacctggccc tgcgctcagc gccgcggctt
     1321 ggtcgcgcgc cacagcgcgc gcagcccgcg cggttcggcg cgcgcggccg ggtgagcccg
     1381 ggtcgcgagc agcgacgagg ccaggtacag gccgttgagc agccggctcg cgcgctcgac
     1441 gctcatgccc ggccagctgc tgatctcgcg cagcgacacg gtctcgcggc gcagccgctg
     1501 cgcggccgag tgcagcgcgc cggccagcgg cgacaccccg tcgacctcga cgctgcgcag
     1561 cgcccggtac gccgcggtgc cggcgatctc ggccagcagg gtcgatcgcg gcccgtcgag
     1621 cgccagtgac cacagcagcg gctgcagcgg ccggtagtgc tgcgccgccg cgatgcgttc
     1681 gtgcatccag tgccccggcg ggcgcacgcc gggcggttcg gtgctcagca gcgccaggcg
     1741 catcagcgac gcgcgcgagg ccagcgccat gtcgcgcggc gagtggtaca gcatctgggc
     1801 cggaaacagt gtgaccggcc acaccaggtc ttcgtactgc aggcacagca gcgccggttc
     1861 gtggtggcgc acgcatgccg ccacgacctc gagcggctcg gcgccggcga caccgccgcc
     1921 gagccggcgc aaatcggcca gcagcgtggt cggcaacacg gtcgcgtggc ccaggcgcgc
     1981 gaaaccgctg ccgccggttt cggcccggac ccgctcgaac tcgctcacct gccagagcgt
     2041 ggactcttca aaatctcgca agcgttcatc gtaggggtgc gctgcgcggc gcgcgttcgc
     2101 agatgtagcg ccgcgcaaga atcggttgcg cgaccggcct gcgcctacag gccgatcacc
     2161 cgcaggccta caaatcggaa cacccctggt gccgcgtcgg cgctaagctt tgtcacaggc
     2221 ctgtgcgctg ggcgccaccc atttcttcac ctccttgatg aacgaccgcc agaccccctc
     2281 gtttgccgct gtcggcggcc tcgccgacgc gaccctgcct gccggcacgc gcctgggcag
     2341 cttcgagttg cagcgcgcga tcgcccgcag cccgagcagc gtcgtctacc aggccaccga
     2401 ccacgcgctg gcgctgacgg tggcgatcca ggagtacctg ccggcgcggc tggtgcggcg
     2461 cgactcgggc ctgcgcctgc gcgccacgga cacctggcac gaggacgtga tcgcgcgcgg
     2521 cctgcgcgcc ttcatcgacg aggcgcgcct gctcgcgcac tgcgaccacc cggcgctggt
     2581 gcgcgtcaac caactgttcg aggtcaacgg cagcgcctac cgggtgatgc cgttctacag
     2641 cggccggcgc ctgctcgacc tgcgccgcgc gatgcccgaa gcgcccgacg aggcctcgct
     2701 gcgcgcgctg ctcgacgacc tgctcggcgc gctcgaggcg atccaccgcg gcggccacgc
     2761 gcacggcggc gtgacgccgg gcaacatcct gttgctcggc gacgaccgcc cgctgctgct
     2821 cgggcccggc gctgcggcgc gcgagatcgg cagcgacctg gtcgattcgc tgatggcgag
     2881 cctggagacg ccgctcgcgc ccgagccggc cgaggtcaca ccgacggggc cgatcgccga
     2941 cctgtacgcg ctcgccgagg tcgtccgctt ctgcatcacc ggcacgccga cgccggtggc
     3001 cgtgccgggc ggcagcggcc gcgaatcgat ggccgaggtc atcgcgcaca gcttcgagcc
     3061 cgccgcgcgg ccgcgctaca gcgccgaact gctgggcacg ctggacgccg cgctgtcgcc
     3121 gctggccgag gactggccgc tcagcgtggc gcagttgcgc acctggctcg cgcgcggcgc
     3181 gccgcgcgcc gccc
//
LOCUS       DATMJB010000101         6936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_223138, whole genome shotgun sequence.
ACCESSION   DATMJB010000101 DATMJB010000000
VERSION     DATMJB010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223138"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VNU48_02250"
     CDS             <1..548
                     /locus_tag="VNU48_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWI10128.1"
                     /translation="ENAPAAQRERLKQEIDTNVAELDALVEEVLLASRLDAAPTPERN
                     ERVELLGVAAEEAARVDALLDGAPITVSGEERLLRRALRNLLENARRYGGGEVEAFVE
                     SQAGHAVVRVCDRGPGVPEAMRERIFEPFFRLPGHAEQGGGVGLGLSLVKQIAERYGG
                     SVRCEAREGGGSCFALSLPLA"
     gene            complement(562..1008)
                     /locus_tag="VNU48_02255"
     CDS             complement(562..1008)
                     /locus_tag="VNU48_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3717 domain-containing protein"
                     /protein_id="HWI10129.1"
                     /translation="MAALHITDIESAINWWRERSPSPDGITACPEVRALGTVYALMVY
                     YHELECDEVTMPPQAREAWLAWYASMPDAPCIAICSTSQGDDICKGCGRSFDEVQRWP
                     VMTPAEKRRTWRRITMEGSAWRFNKYAERVRETPPAPAADARTSQR"
     gene            1106..1573
                     /locus_tag="VNU48_02260"
     CDS             1106..1573
                     /locus_tag="VNU48_02260"
                     /inference="COORDINATES: protein motif:HMM:NF020950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HWI10130.1"
                     /translation="MEPERMTNHPRAWRMLAPGMALALLFGCSTADKPKPARVQAVIQ
                     GAGKAALAVNDVDNGASIVMERSQELIVRLPVRGSSGLDWSLVDLKPGVLRVVSGPTF
                     ERTLLSVADDPSDGSSIWRLRAEAPGNVALNFELRRPHVLGTTRTLSYPVTVK"
     gene            1599..2078
                     /locus_tag="VNU48_02265"
     CDS             1599..2078
                     /locus_tag="VNU48_02265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI10131.1"
                     /translation="MSDQATLHLATRADAAGIAALSRDRIEQGLGWSWTAPRVLRSIR
                     DPATNVVVASRSGRMCGFGIMKYHDDEAHLLLLAVHARCGRRGIGSALVAWLERSALA
                     AGIGQVYLEARLANGAARAFYARLGYREIQTLPGYYQGVEACVRLAKDLWVAPVAPG"
     gene            complement(2167..2565)
                     /locus_tag="VNU48_02270"
     CDS             complement(2167..2565)
                     /locus_tag="VNU48_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10132.1"
                     /translation="MSSLVPDRSAPHAGPDLETALAAVEHRLSALGVALCVRDTVGID
                     LHATELHRALAIAVDQFALAARRGSVPAELRHRLASTSGQVAAQRESLARATAALDRA
                     IDVLMPRDSTALYSTLGSADRGPRGGSVLA"
     gene            complement(2594..2914)
                     /gene="flgM"
                     /locus_tag="VNU48_02275"
     CDS             complement(2594..2914)
                     /gene="flgM"
                     /locus_tag="VNU48_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HWI10133.1"
                     /translation="MKIGHSADKPAPPAAPAAVAATNGSTTAAVAVGTGAIPAQADAS
                     AKIELSNTASTLLTGGTTAEFDAEKVARISKSIEDGSFKINPEAIADKLISNAQELLT
                     KTQS"
     gene            complement(3022..3795)
                     /gene="flgA"
                     /locus_tag="VNU48_02280"
     CDS             complement(3022..3795)
                     /gene="flgA"
                     /locus_tag="VNU48_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HWI10134.1"
                     /translation="MDTRPTPALAAVVRSIRAWWRGAAALLAALAASAALADTAAPAA
                     SSSGLDTGLEQQVRQLALDGSHTGTPGTPRIEVSVGQLDPRLRLAPCTQVQPYIPDGV
                     RLWGKSRIGLRCLQGATKWNVYLPITVKVFGTALVATSGVASGSVLSAADLTPAEVDL
                     AEDNSAPVASAELAVGRTLARALKPGQSLRQSHLKARQWFAAGESVTVVAQGEGFSVA
                     GEAQALNPGIEGQPVRVRTESGRVLTGQPVGERRVELSL"
     gene            3938..4348
                     /gene="flgB"
                     /locus_tag="VNU48_02285"
     CDS             3938..4348
                     /gene="flgB"
                     /locus_tag="VNU48_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HWI10135.1"
                     /translation="MLNRINDALDFQAQALSLRSERQRLIASNIANADTPGYVAREMN
                     FAQALRDATGNGQPAARLAASQSGHIAGNGATSGATFSTLMYAAPSQTNLDRNTVDMD
                     RERASFADNTVKYEATLRFIGAQVKTTLSAITGQ"
     gene            4360..4776
                     /gene="flgC"
                     /locus_tag="VNU48_02290"
     CDS             4360..4776
                     /gene="flgC"
                     /locus_tag="VNU48_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HWI10136.1"
                     /translation="MSMMNIFNVSGSAASAQSQRLNVVASNLANADTVAGPDGQAYKA
                     RQVTFQTQLVGANANDPTAAGVAVSSITEDQTPGRRVLDPKHPSADANGYVTYSNVNA
                     VEEMVNMISASRSYQNNIEVMNTAKSLLLKTLQIGQ"
     gene            4803..5450
                     /locus_tag="VNU48_02295"
     CDS             4803..5450
                     /locus_tag="VNU48_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HWI10137.1"
                     /translation="MAISAATLSNINGTSTSTTAAADDPGSQDRFLKLLVTQLQNQDP
                     LSPMDNAELTSQIAQINTVTGIATLNTSVQGLSSQFLQMQTLQGASLVGKSVIVPGNK
                     IDIASGVGQGGFELATPADAVKVEVLSPAGQVIDTLNMGAQSAGVHGFDWASGNYDNT
                     GNLSFRVTASSGATPLAATALMRDTVNAVSASGTALQLELARNGTVDYSTVKAIN"
     gene            5497..6750
                     /gene="flgE"
                     /locus_tag="VNU48_02300"
     CDS             5497..6750
                     /gene="flgE"
                     /locus_tag="VNU48_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HWI10138.1"
                     /translation="MSFQQGLSGLNASSKNLEVIGNNVANANTIGAKASRAEFGDMYA
                     SAIGTTSIGIGVNLQAVTQQFTQGNITTTDNPLDLAINGTGFFEVSNGAQTLYTRNGQ
                     FQADKDGFLTTNTGLKLVGYPASKSGVIQPGAAVPLQLPTGGVDPNPTSKVTIEMNLD
                     SRKATTLPAAAPQIDFTDASTYNDATSVTVFDAKGQDVALTYYFQKSATDTWNVYATA
                     NGTSVSVDGAGNPLPITTIAYPSNGGTPTAPLGPVAFDVPSTTNAAGATTLPITGVAL
                     DVTGSTQFGSGFGVTSMAQDGYAAGQLTGVTVENSGIVMATYSNGQAKPAGQLELASF
                     RNPQGLSPLGGNLWARSYASGDPTVGAPGSGNIGALQAGALEESNTDLTAELVDMITA
                     QRVYQANAQTIKTQDQVLQTLVNLR"
     gene            6774..>6936
                     /locus_tag="VNU48_02305"
     CDS             6774..>6936
                     /locus_tag="VNU48_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HWI10139.1"
                     /translation="MDRMIYLSMSGAKATMQRQDTLANNLANVSTPGFRAELQAFRAV
                     PVQGSGASTR"
BASE COUNT         1117 a         2462 c         2411 g          946 t
ORIGIN      
        1 tcgagaacgc accggcggcg cagcgcgaac gcctcaagca ggagatcgac accaacgtcg
       61 ccgagctcga cgcgctggtc gaggaggtgc tgctcgcgag ccgcctcgac gccgcgccga
      121 cgcccgagcg caacgagcgc gtcgagctgc tcggcgtcgc cgccgaggag gccgcgcgcg
      181 tcgatgccct gctcgacggt gcgccgatca ccgtcagcgg cgaggagcgg ctgctgcgcc
      241 gcgcgctgcg caacctgctc gagaacgcgc gccgctacgg gggcggcgag gtcgaggcgt
      301 tcgtcgagtc gcaggccggg cacgccgtcg tgcgcgtctg cgatcgcggc cccggcgtgc
      361 cggaggcgat gcgcgaacgc atcttcgaac ccttcttccg cctgccgggt catgccgagc
      421 agggcggtgg cgtcggcctg gggctgagcc tggtcaagca gatcgccgag cgctacggcg
      481 gcagcgtgcg ctgcgaagcg cgcgagggcg gcggcagctg cttcgcgctg agcttgccgc
      541 tggcctgaag agcggcgagc ttcagcgttg tgacgtgcgg gcgtcggccg ccggtgctgg
      601 cggcgtctcg cgcacccgct cggcgtattt gttgaagcgc caggcgctgc cttccatcgt
      661 gatgcggcgc caggtgcgac gcttctcggc cggcgtcatc accggccagc gctgcacctc
      721 gtcgaagctg cggccgcagc ccttgcagat gtcgtcgccc tggctggtcg agcagatcgc
      781 gatgcagggc gcgtcgggca tgctggcgta ccaggcgagc caggcctcgc gcgcctgcgg
      841 cggcatcgtc acctcgtcgc actcgagctc gtggtagtac accatcaggg cgtacaccgt
      901 gccgagtgcg cgcacctccg ggcaggcggt gatgccgtcg ggcgagggtg agcgttcgcg
      961 ccaccagttg atggccgatt cgatgtcggt gatgtgcagt gcggccatgg ggcgccaagc
     1021 atagcgcgca gcggccgcaa actttgatac cctttgagca tgccccctcg actgcctgcg
     1081 cgcagcccac cgacgatcgc gacccatgga accggaacga atgacgaacc acccgcgcgc
     1141 gtggcgcatg ctggcgccgg gcatggcgct cgcgctgctg ttcggttgct cgaccgccga
     1201 caagcccaag ccggcgcggg tgcaagcggt gatccaaggc gccggcaagg ctgcgctcgc
     1261 ggtgaacgac gtcgacaacg gcgcgtccat cgtgatggag cgctcgcagg aactgatcgt
     1321 tcgcctgccc gtgcggggca gcagcggcct ggactggtcg ctggtcgacc tgaagcccgg
     1381 cgtgctgcgc gtggtgtcgg gcccgacctt cgagcgcacg ctgctcagcg tcgccgacga
     1441 cccctccgac ggcagcagca tctggcgcct gcgcgccgag gcgcccggca atgtcgcgct
     1501 gaacttcgag ctgcgccgac cgcacgtgct cggcaccacg cgcacgctga gttacccggt
     1561 cacggtgaag tagcgcgagc ggaccagggg ctgcggcgat gagcgatcaa gcgaccctgc
     1621 acctggccac gcgcgccgac gcggccggca tcgcggcgct gtcgcgcgac cgcatcgagc
     1681 agggcctggg ctggagctgg accgcgccgc gcgtgctgcg cagcatccgc gacccggcga
     1741 cgaacgtcgt ggtcgccagc cggtccggcc ggatgtgcgg tttcgggatc atgaagtacc
     1801 acgacgacga ggcccacctg ttgctgctcg cggtgcatgc gcgctgcggc cgccgcggca
     1861 tcgggtcggc gctggtggcc tggctggaac gctcggcgct cgcggccggc atcgggcagg
     1921 tgtatctgga ggcgcgcctg gcgaacggcg cggcgcgcgc gttctacgcg cgcctgggct
     1981 accgcgagat ccagaccctg cccggttact accagggcgt cgaggcctgc gtgcggctgg
     2041 cgaaggactt gtgggtggcg ccggtggcgc ccggctgacg cggcgccccc gagcgccgcg
     2101 ggtacgccgc tcgacccccg aggggttgcg ggccggctcc ggagcggccg cgcgctcggc
     2161 ccgcgttcag gcgagcacgg aaccgccgcg cgggccgcgg tcggcgctgc cgagggtcga
     2221 atacagcgcc gtgctgtcgc gcggcatcag cacatcgatg gcgcggtcca gcgccgcggt
     2281 ggcgcgcgcc agcgactcgc gctgcgcggc gacctggccg ctggtgctgg ccaggcgatg
     2341 acgaagttcg gccggcaccg agccgcgccg cgcggccagc gcgaactggt cgacggcgat
     2401 cgcgagtgcg cggtgcagtt cggtggcgtg caggtcgatg cccaccgtgt cgcgcacgca
     2461 cagggccacg cccagcgcgc tcagtcggtg ttcgacggcg gccagggcgg tttcgaggtc
     2521 ggggccggcg tgcggcgcac tgcggtctgg aaccagggag gacatggggt gcgggtccgt
     2581 ggtggatgat cgttcaggac tgggttttgg tcagcagttc ctgggcattc gagatcagct
     2641 tgtcggcaat cgcctcgggg ttgatcttga agctgccatc ctcgatggac ttggagatgc
     2701 gggccacttt ctcggcgtcg aactcggccg tcgtgccgcc ggtcagcagc gtggacgcgg
     2761 tgttcgacaa ctcgatcttc gcgctcgcgt ccgcctgtgc cgggatcgcg cccgtgccga
     2821 cggcgaccgc cgcggtcgtg ctgccgttgg tcgcggcgac tgcagcgggg gctgccggcg
     2881 gggcgggttt gtctgcggaa tggccgatct tcatggtgct ctcccagggt cagggcgggg
     2941 cccggaccga tcaatgtcca tggtgtatcg gctcgacccc gcgcgacttt agagatttat
     3001 cctcggcagt ggaagaggtg ttcacaagct cagctcgacc cggcgttccc ccacgggctg
     3061 gccggtgagc actcgcccac tttcggtacg tacccgcacc ggctggccct cgatgccggg
     3121 gttcagcgcc tgggcttcgc cggccacgct gaagccttct ccctgggcga ccaccgtgac
     3181 cgattcgccc gccgcgaacc actgccgcgc cttcaggtgc gactggcgca ggctctggcc
     3241 cggcttcaat gcgcgcgcca gcgtgcgacc gaccgccagt tcggcgctgg ccaccggggc
     3301 cgaattgtcc tcggccaggt cgacctcggc gggtgtcagg tcggcggcgc tcagcacgct
     3361 gcccgacgcg acgccgctgg tcgcgaccag cgcggtgccg aacaccttca cggtgatcgg
     3421 cagatagacg ttccatttgg tcgcgccctg caggcagcgc aggccgatgc ggctcttgcc
     3481 ccacagccgc acgccatcgg gaatataggg ctgcacctgg gtgcagggcg cgagccgcag
     3541 ccgcgggtcg agctggccga cgctgacttc gatgcgcggc gtgccgggcg tgccggtgtg
     3601 gctgccatcg agcgcgagtt ggcgcacctg ctgctccagg ccggtgtcca ggccgctgct
     3661 gctcgccgcc ggcgctgcgg tatcggccag cgcggcggaa gccgcgagcg cggccagcag
     3721 cgccgccgcg ccacgccacc aggcgcggat cgagcgaaca acagcagcaa gggcgggcgt
     3781 cggccgggtg tccatcaccg cactgtaggt gcgcggctgc cggcgcatgg ccgcgaattg
     3841 gccggtgaac cggtcgctat tcccgcttat gttggcgcag gcgctgtcca caatccgctc
     3901 acatcgcccc cttccgactt cgcgacgagg caaccccatg ctgaaccgaa tcaacgacgc
     3961 gctcgacttc caggcccagg ccctgagctt gcgctcggag cgccagcgcc tgatcgccag
     4021 caacatcgcg aacgccgaca cgcccggcta cgtggcgcgc gaaatgaact tcgcccaggc
     4081 gctgcgcgac gcgaccggca acggccagcc cgcggcccgg ctggccgcca gccagagcgg
     4141 ccacatcgca ggcaacggcg cgaccagcgg tgcgaccttc agcacgctga tgtacgccgc
     4201 gccgagtcag accaacctgg accgcaacac cgtcgacatg gaccgcgagc gcgccagctt
     4261 cgccgacaac accgtgaagt acgaggccac gctgcgcttc atcggcgcgc aggtgaagac
     4321 gaccctgtcc gcgatcaccg gtcaataagg aggcacgtca tgtcgatgat gaatatcttc
     4381 aacgtgtcgg gatcggccgc cagcgcgcag agccagcgcc tgaacgtggt cgcgagcaac
     4441 ctggcgaacg ccgacaccgt cgccggcccc gacggccagg cctacaaggc gcgccaggtc
     4501 acgttccaga cccagctggt cggcgccaac gcgaacgacc cgaccgccgc gggcgtggcg
     4561 gtcagcagca tcaccgagga ccagaccccc ggccggcgcg tgctcgaccc gaagcacccg
     4621 agcgccgacg ccaacggcta cgtgacctac agcaacgtca acgcggtcga ggagatggtc
     4681 aacatgatct cggcctcgcg ctcgtaccag aacaacatcg aggtcatgaa cacggcgaag
     4741 agcctgttgc tcaagacctt gcagatcggc cagtaacccc gcctcgcgcc caaggacacc
     4801 ccatggccat ttccgccgcc acgctcagca acatcaacgg cacgagcacc agcaccaccg
     4861 ccgcggccga cgaccccggc agccaggacc gcttcctcaa gctgctggtc acgcagctgc
     4921 agaaccagga cccgctcagc ccgatggaca acgcggagct gaccagccag atcgcgcaga
     4981 tcaacaccgt gaccggcatc gcgacgctga acaccagcgt gcagggcctg tcgagccagt
     5041 tcctgcagat gcagacgctg cagggcgcgt cgctggtggg caagagcgtg atcgtgccgg
     5101 gcaacaagat cgacatcgcc agcggcgtcg gtcagggcgg cttcgagctg gccacaccgg
     5161 ccgacgcggt caaggtcgag gtgctcagcc ccgcgggcca ggtcatcgac acgctgaaca
     5221 tgggcgcgca gagcgccggc gtgcacggct tcgactgggc ctcgggcaac tacgacaaca
     5281 ccggcaacct gagcttccgc gtcaccgcgt cgagcggcgc gacgccgctg gcggcgaccg
     5341 cgctgatgcg cgacaccgtc aatgcggtca gcgcctcggg caccgcgctg cagctcgagc
     5401 tggcccgcaa cggcacggtc gactacagca cggtcaaggc gatcaactga acgcgcgcca
     5461 acggcgaact ccacgaatcc acgaaggaac attcccatga gcttccagca aggtctttcc
     5521 ggtctgaacg cgtcgagcaa gaacctcgag gtgatcggca acaacgtcgc caacgccaac
     5581 acgatcggcg caaaggcgtc gcgcgccgag ttcggcgaca tgtacgcgag cgcgatcggc
     5641 accaccagca tcggcatcgg cgtgaacctg caggcggtga cgcagcagtt cacgcagggc
     5701 aacatcacga ccaccgacaa cccgctcgac ctcgcgatca acggcaccgg cttcttcgag
     5761 gtcagcaatg gcgcgcagac gctgtacacc cgcaacggcc agttccaggc cgacaaggac
     5821 ggcttcctga cgaccaacac cggcctgaag ctggtcggct acccggcctc gaagagcggc
     5881 gtcatccagc ccggcgccgc ggtgccgctg cagctgccca ccggcggcgt cgacccgaac
     5941 ccgacgagca aggtcacgat cgagatgaac ctggactcgc gcaaggcgac gaccctgccg
     6001 gctgccgcgc cgcagatcga cttcaccgac gcgagcacct acaacgacgc gacctcggtc
     6061 acggtgttcg acgccaaggg ccaggacgtc gcgctgacct actacttcca gaagtcggcc
     6121 accgacacct ggaacgtcta cgcgaccgcg aacggcacgt cggtcagtgt cgacggcgcg
     6181 ggcaacccgc tgccgatcac gaccatcgcc tacccgagca acggcggcac gccgaccgcg
     6241 ccgctcggcc cggtggcgtt cgacgtgccg tcgacgacca acgcggccgg cgcgaccacg
     6301 ctgccgatca ccggcgtcgc gctcgacgtg accggctcga cccagttcgg ctccggcttc
     6361 ggcgtgacca gcatggcgca ggacggctat gccgccggcc agctcaccgg cgtcacggtc
     6421 gagaacagcg gcatcgtgat ggcgacctac tcgaacggcc aggccaagcc ggccggccag
     6481 ctcgaactcg cgagcttccg caacccgcag gggctgagcc cgctgggcgg caacctgtgg
     6541 gcgcgcagct acgcgtcggg tgacccgacc gtgggcgcgc ccggcagcgg caacatcggc
     6601 gcgctgcagg ccggcgcact cgaggaatcg aacaccgacc tgaccgccga actcgtcgac
     6661 atgatcaccg cgcagcgcgt ctaccaggcc aacgcgcaga cgatcaagac gcaggaccag
     6721 gtgctgcaga cgctggtgaa cctgcgctga tcctgattcg ctgagggccc gtcatggacc
     6781 gcatgatcta tctgtcgatg tcgggcgcga aggcgacgat gcagcgccag gacacgctcg
     6841 ccaacaacct ggccaacgtg tcgacgccgg gctttcgcgc cgagctgcag gcctttcggg
     6901 cggtgccggt gcagggcagc ggcgcgagca cgcggg
//
LOCUS       DATMJB010000102         2499 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_637322, whole genome shotgun sequence.
ACCESSION   DATMJB010000102 DATMJB010000000
VERSION     DATMJB010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637322"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VNU48_02310"
     CDS             <1..311
                     /locus_tag="VNU48_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWI10140.1"
                     /translation="DPRYKQALVEHAASDIVYSNLFTGVHGNYLGPSIRAAGLDPDRL
                     SESDPSAMNFGGAAFKAWKDIWGCGQGIGAIKAVQPAAELIGRLRREYQAARERVALS
                     "
     gene            526..>2499
                     /locus_tag="VNU48_02315"
     CDS             526..>2499
                     /locus_tag="VNU48_02315"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HWI10141.1"
                     /translation="MMQESRSNSYLFGGNAPYVEEMYEAYLDNPGSVPDNWRSYFDAL
                     QHVPAVDGSTARDVAHAPVVESFAQRAKAGTFGTKASTTDLAIARKQVHVQSLIAAYR
                     SLGARWADLDPLKRQERPKIPELEPAFYDLSESDMDIVFSATNTFFSTADHMTLREIV
                     QALRETYCGAIGAEYMHITTPTEKRWWQERLESIRSKPTYTADQKKHILDRLTAAEGL
                     ERYLHTKYVGQKRFSLEGGESFIVSMDELVQRAGAKGVQEIVIGMAHRGRLNVLVNTL
                     GKMPKDLFAEFDHTAPEALPSGDVKYHQGFSSDITTAGGPVHLSLAFNPSHLEIVNPV
                     VEGSVKARLDRRGDKEGDSVLPIIVHGDAAFAGQGVVMETLALAQTRGYYTGGTVHVV
                     INNQIGFTTSDPRDSRSTLYCTDVVKMIEAPVLHVNGDDPEAVVLCTQLALDYRQEFN
                     KDVVVDIVCFRKLGHNEQDTPSLTQPLMYKKIAQHPGTRKLYGEKLVVQGVLPADGPD
                     AMAKALRAAMDAGKSTYDPVLTNYKSKYAVDWAPFLGKKWTDAADTALPMSEIKRLAE
                     RITTVPPNFKVHTLVEKVLADRAAMGRGEINVDWGMGEHLAFASLVASGYAVRLSGED
                     SGRGTFTHRHAVLHDQNREKFDEGTYVPLQNVAD"
BASE COUNT          459 a          851 c          825 g          364 t
ORIGIN      
        1 ccgatccccg ctacaagcag gcgctggtcg aacatgccgc aagcgatatc gtctattcga
       61 acctgttcac cggcgtgcac ggcaactatc tcggtccgtc aatccgcgcg gccggcctgg
      121 acccggacag gctgtccgag agcgacccca gcgcgatgaa cttcggcggc gccgcgttca
      181 aggcctggaa ggacatctgg ggctgcggcc agggcatcgg cgcgatcaag gccgtgcagc
      241 ccgcggccga actgatcggc cggctgcgac gcgagtacca ggccgcgcgc gaacgcgtcg
      301 cgctgagctg agatcaccgc gacgcgggtg catgcaaggc cggcgcgcgt ccgtttcgcg
      361 caagggtgcg gtggtaaagt cgcggccttg aacttcagag ctccttcccg tcccctcgac
      421 gcttgtggtc gaggcgggtc gcttctccgc ccagtggtct agccgcgtcg cggaaggttt
      481 ttcaacccac tacagtgccg gaaaccggtg cgaaaggtga gcgaaatgat gcaggaatcc
      541 aggtcgaact cgtacctgtt cggcggcaat gccccgtacg tcgaagagat gtacgaggcc
      601 tacctcgaca acccgggctc ggtgcccgac aactggcgca gctacttcga cgcgctgcag
      661 cacgtgcccg cggtcgacgg ctcgaccgcc cgcgacgtgg cccatgcgcc cgtcgtcgag
      721 tcattcgctc agcgcgccaa ggccggcacc ttcggcacca aggcctcgac caccgacctg
      781 gcgatcgcgc gcaagcaggt ccatgtccag tcgctgatcg cggcctatcg ctcgctcggc
      841 gcgcgctggg ccgatctcga cccgctcaag cgccaggagc ggccgaagat ccccgagctc
      901 gagccggcgt tctacgacct gagcgagtcc gacatggaca tcgtgttcag cgcgaccaac
      961 accttcttct cgaccgccga tcacatgacg ctgcgcgaga tcgtgcaggc gctgcgcgag
     1021 acctattgcg gcgcgatcgg cgccgagtac atgcacatca ccacgccgac cgagaagcgc
     1081 tggtggcagg agcggctcga atcgatccgc tcgaagccga cctacacggc cgaccagaag
     1141 aagcacatcc tcgatcgact gaccgccgcc gaaggcctgg agcgctacct gcacaccaag
     1201 tacgtcggcc agaagcgctt ctcgctcgaa ggcggcgaga gcttcatcgt ctcgatggat
     1261 gaactggtgc agcgcgccgg cgcgaagggc gtgcaggaga tcgtgatcgg catggcgcac
     1321 cgcggccggc tgaacgtgct ggtcaacacg ctgggcaaga tgcccaagga cctgttcgcc
     1381 gagttcgacc acaccgcgcc cgaggccctg ccgtcgggtg acgtgaagta ccaccagggt
     1441 ttctcgtccg acatcacgac ggccggcggc ccggtccacc tgagcctcgc gttcaacccc
     1501 tcgcacctcg agatcgtgaa cccggtcgtc gaaggctcgg tcaaggcgcg cctcgaccgg
     1561 cgcggcgaca aggaaggcga ctcggtgctg ccgatcatcg tccacggcga cgcggctttc
     1621 gccggccagg gtgtcgtgat ggaaacgctg gcgctggcgc agacgcgggg ctactacacc
     1681 ggcggcacgg tgcacgtcgt catcaacaac cagatcggct tcacgaccag cgacccgcgc
     1741 gactcgcgct cgacgctgta ctgcaccgac gtggtcaaga tgatcgaggc gccggtgctg
     1801 cacgtgaacg gcgacgaccc cgaggcggtc gtgctgtgca cgcagctggc gctcgactac
     1861 cggcaggaat tcaacaagga cgtggtcgtc gacatcgtct gcttccgcaa gctgggccac
     1921 aacgagcagg acacgcccag cctgacgcag ccgctgatgt acaagaagat cgcccagcac
     1981 cctggcacgc gcaagctgta cggcgagaag ctggtggtgc agggcgtgct gccggccgac
     2041 ggccccgatg cgatggcgaa ggcgctgcgc gcggcgatgg acgccggcaa gagcacctac
     2101 gacccggtgc tgaccaacta caagagcaag tacgcggtcg actgggcgcc gttcctcggc
     2161 aagaagtgga ccgatgcggc cgacaccgcg ctgccgatgt ccgagatcaa gcgcctggcc
     2221 gagcgcatca cgacggtgcc gccgaacttc aaggtccaca cgctggtcga gaaggtgctc
     2281 gccgaccgcg ccgcgatggg ccgtggcgag atcaatgtcg actggggcat gggcgagcac
     2341 ctcgcgttcg cgtcgctggt ggccagcggc tacgcggtgc ggctgtcggg cgaggactcc
     2401 gggcgcggca cgttcacgca ccgccacgcc gtgctgcacg accagaaccg cgagaagttc
     2461 gacgaaggca cctatgtgcc cttgcagaac gtcgccgac
//
LOCUS       DATMJB010000103         2437 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_103963, whole genome shotgun sequence.
ACCESSION   DATMJB010000103 DATMJB010000000
VERSION     DATMJB010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103963"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VNU48_02320"
     CDS             <1..137
                     /locus_tag="VNU48_02320"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HWI10142.1"
                     /translation="EDPTAMARAILLGVEGGRLAYRAGRIPRRLHAQASTPEEGVAAF
                     "
     gene            404..2176
                     /locus_tag="VNU48_02325"
                     /pseudo
     CDS             404..2176
                     /locus_tag="VNU48_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466854.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2173..>2437
                     /locus_tag="VNU48_02330"
     CDS             2173..>2437
                     /locus_tag="VNU48_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI10143.1"
                     /translation="MSLLKVTGLGKSFGGNRAVDGVGFEVVPGELLALIGPNGAGKST
                     TFNMVNGQLRADSGSIQLDGKELIGRKPRDIWHMGVGRTFQITA"
BASE COUNT          274 a          843 c          896 g          424 t
ORIGIN      
        1 ccgaggaccc gaccgcgatg gcgcgggcta tcctgctggg cgtcgagggc gggcggctcg
       61 cctatcgcgc cgggcgcatc ccgcgccgcc tgcacgcgca ggcgtccacc cccgaggagg
      121 gggtcgcggc gttctgagca ccgcggacga gctgttcgac ggttgggagc gggcctggtc
      181 cgggcgggat cccgcggcct tcgatcccct ctgccacccc gaggtccact acgaggatcc
      241 gctcctgccc gagccgctgg agggcgccga cgcgatcgcc cggcgcgcgg cgaaggtctg
      301 ggaggcgttc cccgacgcgc ggctccagcc gatcgggccc cggctcagcg acggcactca
      361 cgcgagcgcg ccggcgaagc tgctgggcac gcatcgcgga atcgcgcacg gctcgttcta
      421 catgctgggc gtgtacatcg cctacatgct gaccgagcgt ctcggcggcg gcgcgtggtt
      481 ctggcccgcg ctgctgctcg cggcgctcgc ggtcggcgcg atcggtgcgc tggtcgaagt
      541 gctgctgctg cgacgtgtct accgcgcccc ggagctgttc cagctgctcg cgaccttcgc
      601 gctgatgctc gtgatcaagg acgccgcgct gtggctgtgg ggcccggaag acctgctcgg
      661 tccgcgcgcg cccggcctcg cgcatgcggt gccgattctg ggccgcatgt tcccgaccta
      721 cgatttgttc ctgatcgtcg tcggcccgct cgtgctcgga gctctgtggc tgctgctgac
      781 gcgcacgcgc tggggcacgc tggtgcgcgc cgcgacacaa gaccgcgaga tggtcggtgc
      841 cctcggcgtc aaccaggcct ggctgttcac cgcggtgttc gcgctcggca cgatgctcgc
      901 cggcctgggc ggcgcgctgc agttgccgcg cgagccggcc aacctgggcc tggacctgca
      961 gaccatcggc gatgccttcg tcgtcgtcgt ggtcggcggc atggggtcga tccccggcgc
     1021 gtatgtcgcc gcgctgctga tcgccgagat caaggcgatc tgcatcggca tcggcacggt
     1081 cgatgtgctg gggatcagct tctcgttctc gaagctgacg ctggtggtcg agttcctcgt
     1141 gatggccgtc gtgctggtcg tgcggccctg gggcctgatg gggcggccgc aggcggtgag
     1201 tcgcaactcg gcgccggccg aggcgccgct gcgccgggcc gaaacgccgc tgaagatcgc
     1261 cggcgtgctg ctgctgatcg cgctgctggc gtggccggtg gcgaccgcga gctcgccgta
     1321 cctcaccgtg ctcgcgatcg acttgctcac cgcggcgctg ttcgcggcca gcctgcactt
     1381 catcatgggc ccggccggca tgcattcgtt cggccacgcc gcgtacttcg gcctcggtgc
     1441 ctacggcgcg gcgctgctgg tgcgcgcggc gcatctgccg atggagttga cgctggtgct
     1501 gtcgccgttc gtcgccgctg gcggcgcgct ggtgttcggc tggttctgcg tgcggctgtc
     1561 gggcgtgtac ctggcaatgc tgacgctcgc gttttcgcag atcgtctggt cgatcgtgtt
     1621 ccagtgggac gacttcaccg gcggcagcaa cggcctgacc ggcgtctggc cggccgaatg
     1681 gctgagcgac aagcgcagct actactacct gacgctctgc cttgtggtcg gcggcgtgct
     1741 gctgatccgc cgcgcgctgt tcgcgccctt cggctacgcg atgcgcgcgg ggcgcgactc
     1801 gccgctgcgt gccgacgcca tcggcatcga tgtgaagcgc atccagtggg ccgcgttcgt
     1861 gatcgccgcg ctgttcgccg ggctggccgg cgcgctctac gcgttctcga agggcagcat
     1921 ctcgccggac acggtggcgg tcggccgctc cgtcgacggc ctcgtgatgg tgctgctcgg
     1981 cgggctgcag acgctcgcgg gcccgatcgt cggcgcggtc acgttcacct ggctgcagga
     2041 cgccgtggcg cgcaacaccg actactggcg cgcgctgctc ggcggcatca tcctgctgct
     2101 ggtgctggcg ttcccgcaag gcatcgcggg cttcgtcggc gggctgttcg agcggcgcaa
     2161 gggcgcgaac gcatgagcct gctgaaagtc accggcctgg gcaagtcgtt cggcgggaac
     2221 cgcgcggtcg acggcgtggg cttcgaggtc gtgcccggcg agttgctcgc gctgatcggg
     2281 ccgaacggcg ccggcaagtc gaccaccttc aacatggtca acggccagct gcgcgccgac
     2341 tcgggctcga tccagctcga cggcaaggag ctgatcgggc gcaagccgcg cgacatctgg
     2401 cacatgggcg tcggccgcac gttccagatc accgcga
//
LOCUS       DATMJB010000104         5801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_696191, whole genome shotgun sequence.
ACCESSION   DATMJB010000104 DATMJB010000000
VERSION     DATMJB010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5801
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696191"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..917
                     /gene="rsgA"
                     /locus_tag="VNU48_02335"
     CDS             12..917
                     /gene="rsgA"
                     /locus_tag="VNU48_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HWI10144.1"
                     /translation="MSKTVALDLGLVVAGHGRHYIVETPDGERVICHPRGKKSDCVVG
                     DRVRWQASGDEGVIEHVEPRRNLLFRQDEWKTKSFAANLDQLMVLVAAEPVFSESQLA
                     RALIAAESAGIPARIVLNKADLPTIVVARERLAPYAAMGIEIVELALKAQPGAARAAL
                     APLLDGRATLVLGPSGTGKSTLINLLAPDAKAQVGEISQALNSGRHTTTTTQWYWIDG
                     DERRTALIDSPGFQEFGLRQIDAAQLPALMPDLRLHASTCRFYNCTHLHEPGCGVRAA
                     LERGEISESRYRIYGEILAELVQRR"
     gene            complement(914..1903)
                     /locus_tag="VNU48_02340"
     CDS             complement(914..1903)
                     /locus_tag="VNU48_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CobD/CbiB family protein"
                     /protein_id="HWI10145.1"
                     /translation="MSFFAVLFALLIEQLKPLPRSNVVHDSLTGWMRWTGRNFDAGRD
                     HHAWVVWSITALFPALVAWGVHAGLGHVNLLLALVWNVAVLYLTLGFRQFSHYFTDIR
                     DALERGDEAEARRLLSEWRHLDASELPRTELLRHVIEHSLLAAHRHVFGVFFWFVLLS
                     TFGFGPAGAVLYRMAEYASRYWAFKSRTIDAPVNLRLLELSQRLFSALDFVPARLTAF
                     GFAVVGNFEEAVTCWRRDAGLWKHPNEGIILAAAAGAVGVQLGGSAAPGVTPDRSQTF
                     EAGVAPEAVTAEGSTPGLPPVLGHLRSVVGLVWRSVVLWMLLLALLSLANLVG"
     gene            complement(1936..2619)
                     /locus_tag="VNU48_02345"
     CDS             complement(1936..2619)
                     /locus_tag="VNU48_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HWI10146.1"
                     /translation="MPLSFDPQKLPVIGIDDHLPALTPAVLHGDALRQRFLTPPVWTP
                     EIKTEGRFTERAPAHASVLIALVPRNEITVLLTQRTDHLTDHPGQISFPGGRVDAGDA
                     DAAATALREAHEEIGLETPFVDVLGALPTYTTGTGFIVTPVVALVRPGFTVQAEPFEV
                     AEVFEVPLSFLMNPANHRRHAVEVNGTSRQFLSIPWDGLDEAGQPRRYFIWGATAAML
                     RNLYRFLAA"
     gene            complement(2774..3127)
                     /gene="rplS"
                     /locus_tag="VNU48_02350"
     CDS             complement(2774..3127)
                     /gene="rplS"
                     /locus_tag="VNU48_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWI10147.1"
                     /translation="MDLIQTLEREEISRLNKTIPEFAPGDTVIVSVKVVEGTRKRLQA
                     FEGVVIAKRNRSLNSSFIVRKISSGEGVERTFQLYSPLIASIEVKRRGDVRRAKLYYL
                     RSRSGKSARIKEKLA"
     gene            complement(3252..4010)
                     /gene="trmD"
                     /locus_tag="VNU48_02355"
     CDS             complement(3252..4010)
                     /gene="trmD"
                     /locus_tag="VNU48_02355"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWI10148.1"
                     /translation="MRFDVITLFPELFAPHLTQGITRRAFESGQVEVRLWPLRDFADN
                     AYRRVDDRPYGGGPGMVMLVEPLERALAAVRAARGDAVKAPLVHFTPTGRRIDQAVVD
                     EFAKSPGAVLLCGRYEGIDQRFIDRHVDVELSLGDFVLSGGELPALALLDAVARLQEG
                     VLGDAQSHQQDSFSDGLLDCPHYSRPELLGSGDADRSIPEVLLSGHHAEIARWRREQS
                     LALTARRRPDLISAARAAGRLSVADERYLASLVT"
     gene            complement(4023..4628)
                     /gene="rimM"
                     /locus_tag="VNU48_02360"
     CDS             complement(4023..4628)
                     /gene="rimM"
                     /locus_tag="VNU48_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296004.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWI10149.1"
                     /translation="MSAAARPLGADPGWPEDAIEVGRIVDAWGLKGWIKVQPFAADPQ
                     ALFSSRRWFLLPSEKVGPLRPAGVTSIPSLLRITQVKDHGDVVVALAQEVPDRSAAEA
                     LRGARVFIGRASFPSAGPDEFYWVDLIGLAVVNRAGERLGTVAGLIDTGPHSVLRVTP
                     DSVPAGVSAQQAERLIPFVGAYVDEVSLEQRVVTVDWGLDY"
     gene            complement(4663..4911)
                     /gene="rpsP"
                     /locus_tag="VNU48_02365"
     CDS             complement(4663..4911)
                     /gene="rpsP"
                     /locus_tag="VNU48_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWI10150.1"
                     /translation="MVVIRLSRGGAKKRPFFNIVVADSRTRRDGRFIERVGFYNPVAS
                     GGEQPLRVAFDRIEHWTSTGAQLSPTVQRLVAQAKAAA"
     gene            5106..5648
                     /locus_tag="VNU48_02370"
     CDS             5106..5648
                     /locus_tag="VNU48_02370"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI10151.1"
                     /translation="MSSPPLLIRPSTPADLPAITAIYGWNVVHGTGTFELDAPDEAEM
                     SRRRDDVLAKGLPWLVVERAGTVQGYAYANHFRPRRAYRFCVEDSLYLAADARGQGLG
                     RLLLVELMARCEAAGARQMLAVIGDSANLGSVGVHRTLGFEHIGVMKAAGWKFDRWLD
                     VVVMQKALGPGATTAPWDAP"
BASE COUNT          966 a         1992 c         1994 g          849 t
ORIGIN      
        1 tgggggcgcc attgagcaag accgtcgcgc tcgacctcgg gctggtggtc gcggggcacg
       61 gccgccacta catcgtcgag acacccgatg gcgagcgcgt catctgccac ccgcgcggca
      121 agaagagcga ttgcgtcgtc ggtgaccgcg tgcgctggca ggcctcgggc gacgagggcg
      181 tgatcgagca tgtcgagccg cgccgcaacc tgctgttccg gcaggacgaa tggaagacca
      241 agtcgttcgc cgcgaacctc gaccagctga tggtgctggt cgctgccgag ccggtattca
      301 gcgagtcgca actggcgcgc gcgctgatcg cggccgagag cgccggcatc ccggcgcgga
      361 tcgtgctgaa caaggccgac ctgccgacga tcgtcgtcgc ccgtgagcga ctcgcgccgt
      421 atgcggcaat gggcatcgag atcgtcgaac tcgcactgaa ggcgcaaccc ggcgccgccc
      481 gtgccgcgct ggcgccgctg ctcgacggcc gcgcgacgct ggtgctcggg ccgagcggca
      541 ccggcaagag cacgctgatc aacctgctcg cacccgacgc gaaggcgcag gtcggcgaga
      601 tctcgcaggc gctgaactcc ggccggcaca cgacgacgac cacgcagtgg tactggatcg
      661 acggcgacga acgccgcacc gccctgatcg actcgccggg cttccaggaa ttcggtctgc
      721 gccagatcga tgccgcgcag ctgccggcgc tgatgccgga cctgcgcctg cacgcatcga
      781 cctgccgctt ctacaactgc actcacttgc acgaacccgg ctgcggcgtg cgcgctgcgc
      841 tcgagcgggg cgagatcagc gagtcgcgct accggatcta cggcgagatc ttggccgagc
      901 tggtgcagcg ccgctagccg acgaggttcg ccaatgacag cagcgccagc agcagcatcc
      961 acaacaccac cgagcgccag accaggccga cgacgctgcg caggtggccg agcaccggcg
     1021 gcaggccggg cgtcgagccc tcggccgtca ccgcctcggg tgcgaccccg gcctcgaagg
     1081 tctgtgaacg atccggcgtg acacccggtg cggcgctgcc gcccagttgc acgccgaccg
     1141 cgccggcggc ggccgcgagg atgatgcctt cgttcgggtg cttccacagg cccgcatcac
     1201 ggcgccagca ggtcaccgct tcctcgaagt tgccgaccac cgcgaagccg aaggccgtga
     1261 gccgcgccgg cacgaagtcg agcgcgctga acaggcgctg cgacagttcc agcaggcgca
     1321 ggttcacggg ggcgtcgatc gtgcggctct tgaaggccca gtagcggctc gcgtactcgg
     1381 ccatgcggta cagcaccgcg ccggccgggc cgaagccgaa ggtcgacagc agcacgaacc
     1441 agaagaagac cccgaacaca tggcgatgcg cggccagcag cgaatgctcg atcacgtggc
     1501 gcagcagctc ggtgcggggc agttcgctcg cgtcgaggtg gcgccattcg gacagtaggc
     1561 gccgcgcctc ggcttcgtcg ccgcgttcga gcgcgtcgcg gatgtcggtg aaatagtggc
     1621 tgaactggcg aaagcccaac gtgaggtaga gcaccgccac gttccacacc agcgccaaca
     1681 gcaggttgac gtgccccagg cccgcatgca cgccccaggc cacgagggcc gggaacagcg
     1741 cggtgatgct ccagacgacc cacgcgtggt ggtcgcgccc ggcatcgaag ttgcggccgg
     1801 tccagcgcat ccagccggtc aacgagtcgt gcaccacgtt gctgcgcggc agtggtttga
     1861 gttgctcgat cagcagcgcg aacaggacag cgaagaagct catgcgagcc cgatgatagc
     1921 gacgcccctg ctgactcagg ccgcgagaaa gcgatagaga ttgcgcagca tcgccgcggt
     1981 cgcgccccag atgaaatagc gccgcggctg gccggcctcg tccagcccat cccacgggat
     2041 cgacaggaac tggcgcgacg tgccgttcac ttcgaccgcg tggcgccggt ggttcgccgg
     2101 gttcatcagg aacgacagcg gcacctcgaa cacctcggcc acctcgaagg gctcggcttg
     2161 caccgtgaag ccggggcgca ccagcgcgac caccggggtc acgatgaagc cggtgccggt
     2221 ggtgtaggtc ggcagcgcgc cgagcacgtc gacgaacggc gtctcgagac cgatctcttc
     2281 gtgcgcctcg cgcagcgccg tggccgccgc atcggcgtcg cccgcgtcga cgcggccgcc
     2341 cggaaagctg atctgacccg ggtgatcggt caggtgatcg gtgcgctgcg tcagcagcac
     2401 ggtgatctcg ttgcgcggca ccagtgcaat cagcaccgac gcgtgcgccg gtgcacgctc
     2461 ggtgaagcgc ccttcggtct tgatctcggg cgtccagacc ggcggcgtga ggaaacgctg
     2521 gcgcagcgcg tcgccgtgca gcaccgccgg cgtcagcgcc ggcaggtgat cgtcgatgcc
     2581 gatgacgggc agtttctgcg ggtcgaagct cagaggcatg aagaatcggt gaaaggcaaa
     2641 aggccgccca tcgtggcggc ctcgaggcag tgggatctca ggccgacttt tgcagcgtct
     2701 ggccgtgcca gtcgctgcga aagctcgctt cgcactgcgt gcgagcatcg tcttgcgacg
     2761 atgcctgact tcgtcaggcg agcttttcct tgatgcgcgc cgacttgccc gagcggctgc
     2821 gcaggtagta cagcttcgcg cggcgcacat cgccgcggcg cttgacctcg atcgatgcaa
     2881 tcagcgggct gtacagctgg aacgtgcgct ccacgccttc gccgctggag atcttgcgca
     2941 cgatgaagct ggagttcagg ctgcgattgc gcttggcgat cacgacgcct tcgaaggcct
     3001 gcagccgctt gcgcgtgcct tcgacgacct tgacgctgac gatcacggtg tcgcccggcg
     3061 cgaattcggg gatggtcttg ttcaggcgcg agatttcttc gcgctcgagg gtctggatca
     3121 gatccatggt ttctccaaag tgatcatgcc aacgcgttga tggagttggg tggagcggcg
     3181 accgcagtcc gcagattcat ctggcggctc gtctgtgctc acggcggggc agaggatcga
     3241 aaagcctttg attatgtcac aagcgaggcc agataccgct cgtcggccac gctgagccgc
     3301 cccgccgccc gcgctgcgga gatcaggtcg gggcgccggc gggcggtcag tgccagcgac
     3361 tgctcacgcc gccagcgcgc aatctcggcg tgatggccgg acaacagcac ctcgggaatc
     3421 gaccgatcgg cgtcgcccga gcccagcagt tcggggcggc tgtagtgcgg gcagtcgagc
     3481 aggccgtcgg aaaaactgtc ctgctggtgc gactgcgcgt cgcccagcac gccttcctgc
     3541 aggcgtgcga cggcatccag cagcgccagc gccggcaatt cgccgcccga cagcacgaag
     3601 tcgcccaggc tcaattccac atcgacatgg cggtcgatga agcgctggtc gatgccctca
     3661 tagcgcccgc acagcagcac ggcgccgggc gatttcgcga actcgtcgac gacggcctga
     3721 tcgatgcgtc gcccggtcgg cgtgaagtgc accagcggcg ccttcacggc atcgccgcgc
     3781 gccgcacgga cggccgcgag cgcgcgctcg agcggctcga ccagcatcac catgcccggc
     3841 ccgccgccat acgggcgatc gtcgacgcgg cggtaggcgt tgtcggcgaa gtcgcgcagc
     3901 ggccataacc tcacctcgac ctggccggat tcgaacgcgc gccgcgtgat gccctgcgtc
     3961 aggtgcggtg cgaacagttc cgggaacaac gtgatgacgt cgaagcgcat ggtcgtccgc
     4021 gcctagtagt ccaggcccca gtcgaccgtg accacgcgct gctcgaggct gacctcgtcg
     4081 acgtaggcgc cgacgaacgg aatcagccgc tcggcctgct gcgcgctgac gccggccggc
     4141 acgctgtccg gcgtcacgcg cagcacgctg tgcgggccgg tgtcgatcag cccggccacg
     4201 gtgccgagcc gctcgcctgc acggttgacg accgccaggc cgatcaggtc gacccagtag
     4261 aactcgtccg gcccggcgct cgggaagctg gcgcggccga tgaacacgcg cgcaccgcgc
     4321 aaggcttcgg cagcgctgcg atcgggcact tcctgcgcca gcgccacgac gacgtcgccg
     4381 tgatccttga cctgcgtgat gcgcagcaac gagggaatcg aggtgacgcc cgcgggacgc
     4441 aacggcccga ccttctcgga cggcagcaga aaccagcggc gcgacgagaa cagcgcctgc
     4501 gggtcggccg cgaagggctg aaccttgatc cagcccttga ggccccacgc gtcgacgatg
     4561 cgaccgactt cgatcgcgtc ctcgggccag ccgggatcgg cgccgagcgg acgggcagca
     4621 gcgctcacgg cccgcagccc ggcgtacccg tcaggcgacg actcaggccg cggccttggc
     4681 ctgcgcgacc agacgctgaa ccgtcggcga gagttgcgcg ccggtgctgg tccagtgttc
     4741 gatgcggtcg aacgccacgc gcagcggctg ctcgccgccc gaggcgaccg ggttgtagaa
     4801 gccgacgcgc tcgatgaagc ggccgtcgcg gcgggtgcgc gagtcggcga cgacgatgtt
     4861 gaagaacggg cgcttcttgg cgccgccgcg ggagagtcga atcacgacca tgattgttcc
     4921 ttcatgtgta cagagcatcc accgcaccag gacaccggaa cctcgataga acgtcgatga
     4981 acgatcgggg tcacgcaggc attagcagat cgggatccat cgtcgctgta gatacgccgg
     5041 ccactcggcc gaccagcaac gaaaccgcgc attataaact cagcgatcgc cacgtcggac
     5101 aactcatgag cagccctccc ttgctgattc gccccagcac ccccgccgac ctgcccgcca
     5161 tcaccgcgat ctacggctgg aacgtggtgc acggcaccgg caccttcgaa ctcgacgccc
     5221 ccgacgaagc cgaaatgtcg cgccgccgcg acgacgtact ggccaagggc ctgccctggc
     5281 tggtggtcga gcgcgccggc acggtgcagg gctatgccta tgccaaccac ttccggccgc
     5341 ggcgggccta ccgcttctgc gtcgaggact cgctctatct ggccgccgac gcccgaggtc
     5401 agggcctcgg ccggctgctg ctggtggagc tgatggcgcg ctgcgaagcg gccggcgcgc
     5461 gccagatgct cgcggtgatc ggcgattcgg ccaacctcgg ctcggtcggc gtgcaccgca
     5521 cgctgggctt cgagcacatc ggcgtgatga aggcggccgg ctggaagttc gaccgctggc
     5581 tcgacgtcgt cgtgatgcag aaggcgctcg gcccgggcgc cacgaccgcc ccgtgggatg
     5641 cgccatgacg ggcaccgcgt cacgctacaa gtccaagacg ctggcgacct ggttcgcgct
     5701 gctcggcggc acggtcggtc tgcaccgctt ctacctgcac gggtttcgcg atcgctgggg
     5761 ctggctgttc gtgtggccca gcctggtcgg cctgtacggc g
//
LOCUS       DATMJB010000105         6189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_815068, whole genome shotgun sequence.
ACCESSION   DATMJB010000105 DATMJB010000000
VERSION     DATMJB010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815068"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VNU48_02375"
     CDS             <1..389
                     /locus_tag="VNU48_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10152.1"
                     /translation="MWIDGVRPNLARLAETFDPAHMDILLLMAPTTSSIGYGGRGDYA
                     MLPDGALRKRKEHQVVPFVYAGAAIMSPSLFADAPAGEFSLTKLFDRANEQERLFGLR
                     LDGVWMHVGTPDAVHAAEEAFLESVA"
     gene            578..3724
                     /gene="addB"
                     /locus_tag="VNU48_02380"
     CDS             578..3724
                     /gene="addB"
                     /locus_tag="VNU48_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HWI10153.1"
                     /translation="MRVFSVPVSVPFLRSVIAALVDGRLVDGFEARTQPDRLAQATLY
                     LPTRRAGRMAREIFLDELKRDAVLLPRIVALGDIDEDELGFTEDSEQYGGTAPLDIPP
                     KLGELDRRLTLAKLVGAWAKGPVLAPLVVGGPASTLALAADLARLMDDMVTRGVGWDA
                     LDGLVPDQLDEYWKYSLEFLRIARLAWPAHLAEIQKIEPAARRDLLIEAEAARLTSHH
                     HGPVIAAGSTGSMPATAKFLHAVANLSHGAVVLPGLDTDLDDDAWHLIGGVRDALGTF
                     TTHPASNHPQFAMYSLLQRFGIKRADIEILGAPSPRGRDVLVSEAMRPSTATAHWHER
                     LKQPEIVAKIAAGMTDLAVIEAANPEMEALAIAVAMREARHLGKSAALVTPDRALARR
                     VTAALDRWNLEFDDSGGDALMDTSAGIFARLAAEAVTKGLEPPTLLALLKHPLCRMGG
                     AEGAISGAVEILELALLRGTRPQAGSAGLARDFALFRSELAKLRREEESSLHRSEQRT
                     RLSVEDLDRAQSLIAQLQKALSPLEVLSSSKPYDFAELAERHREMLTELSRDPHGAAL
                     AFEGQQGTGLVAAFDDLIGAQTRSGLMVRLGDYPEVFQTAFADRMVRRPESANAQLHI
                     YGQLEARLTQSDRVILGGLVEGVWPPAPRIDPWLSRPMRHELGLDLPERRIGLSAHDF
                     AQLLGSDEVILSHAAKVGGAPAVASRFLHRLEAVAGEDHWKAAKAAGDKYVLFANELD
                     RPAKVVPIPQPRPRPPRAARPLKLSVTAIEDWLRDPYTIYAKYILRLSPLDPVDMPLS
                     AADRGSAIHDALGKFTQSYSTALPDNPALALREIGEKSFAPLMERPEAKALWWPRFQR
                     IAAWFAGWETVRRGDVVSIDAETRGNISIRLDNERTFELSARADRIERRRDGTFAILD
                     YKTGQPPTGKQVRMGLSPQLTLEAAILREGGFKEIAADSSVSELVYVRLSGNNPPGEQ
                     RLLELKINQKEQPQSPDAAADEARLKLEALIRAFEDESQAYTSLNLPMWTSRYGSYDD
                     LARIKEWSAAGGLEIPEW"
     gene            3718..>6189
                     /gene="addA"
                     /locus_tag="VNU48_02385"
     CDS             3718..>6189
                     /gene="addA"
                     /locus_tag="VNU48_02385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HWI10154.1"
                     /translation="MVKSVRQIPDAVRDTQRRASDPTSSAFVSANAGSGKTHVLVQRV
                     IRLLLAGVPPEKILCITFTKAAAANMAERVFTTLGHWITLDDEALDDAISDVGIANPN
                     LKLRMRARELFACALETPGGLKVQTIHALCTRLLQQFPFEANVPARFAVLDDRDQNEM
                     MERANLAVLLEASRNPDSATGRALDVAMASAADVTFKDVVREACLSRDHFMAWTDDAG
                     SAEVAAAQMAAALGVSADDRIEDLEREITDGPYLRRSEWEEIVTVLETGSKSDQDQAA
                     RLRAALLVTGSAQVDAYISLFLTDTERTPRKTVVTKKLADNHPAVARLFEAEFLRIGP
                     LIERRRAVTTRDRTQALLHIATAAAANYRREKQERGLLDYDDLIDKTLAMLDRVSSGW
                     VHYKLDRGVDHVLIDEAQDTSPRQWDIVAHIISEFTSGAGARDGVVRTVFAVGDEKQS
                     IFSFQGAAPREFDLRRRALQRKFEGAGLKFDPVSFTYSFRSGPEILHAVDQVFREQDI
                     FRSIHAVDIGNPIHHALADAGPGQIDLWELAETDDRQDIEGWRAPFDGVAATSPEVKL
                     ARRIQAEIKTLVGSGVMTGSKNDRRPLRYGDMLVLVRRRGNAFDAVIQALKHAGIPVA
                     GADRLKLTEHIAIIDLMNLADALLLPQDDLALAVALKSPLFALTDDDLFKIAWQRKGS
                     LRAALAERAAADGRLRDALWRLEQCERRFASETPFAFYAWLLGGDGGRARILRRLGHE
                     ANDALDEFLELALNHERKAPASLQGFMAWLRTADLEVKRDMEISRDEVRVMTVHGAKG
                     LEASVVIMADTTTSPSDTQRLRLIHL"
BASE COUNT         1096 a         1995 c         2067 g         1031 t
ORIGIN      
        1 ccatgtggat cgacggcgtg cggcccaatc tggcacggct tgccgaaacc ttcgatcccg
       61 cgcacatgga tatcctgctg ctgatggcgc cgaccaccag cagcatcggc tatggcggcc
      121 gcggcgacta cgctatgctg ccggacggcg cgctgcgcaa gcgcaaggaa catcaggtgg
      181 tgccgttcgt ctatgccggc gccgcgatca tgtcgccgtc gctgtttgcc gatgcacccg
      241 ccggcgagtt ctcgctgacc aaactgttcg accgcgccaa tgagcaggaa cggctgttcg
      301 gcctgcggct cgatggcgtc tggatgcatg tcggaacccc cgacgcggtt cacgccgccg
      361 aagaagcgtt cctggaaagc gtcgcgtaac cagcggtcgt ctcgcgtagc ggcatccagg
      421 acgccgcggc ttctcggctc cacgtcgttt tctctgggat acaggatcat ccgctcgcgc
      481 gatgatgatg acagagaacg gtgtttcccg tccccgccgc ccatgaccaa tcgcaaatcg
      541 ctccctatat tggcgctgat ctcgaatcag gaagcccatg cgtgttttca gcgttcccgt
      601 ctccgtgccg ttcctgcgca gcgtcatcgc agcgctggtc gacggcaggc tggtcgacgg
      661 ctttgaggcc cgcacgcagc cggacagact ggcgcaggcg acgctctatc tgccgacccg
      721 acgcgccggc cgcatggcgc gggaaatctt tctcgacgag ctgaagcggg atgcggtgtt
      781 gctgccgcgc attgtcgcgc tcggcgacat cgacgaagac gaactcggct tcaccgagga
      841 cagcgagcaa tatggcggga cggctccgct cgacattcca ccgaagcttg gtgaactcga
      901 tcgcaggctg acgctggcaa aactggtcgg cgcttgggca aaaggtcccg tgctggcgcc
      961 gctggtcgtc ggcggcccgg cctccacgct ggcattggcg gccgacctgg cgcggctgat
     1021 ggacgacatg gtgacgcgag gcgtcggctg ggacgcgctt gacgggctgg tgccggatca
     1081 gctcgacgaa tactggaaat actcgctcga attcctgcgg atcgcgcggc tggcgtggcc
     1141 ggcgcatctc gccgagatcc agaaaatcga gccggcggcg cggcgcgacc tgctgatcga
     1201 ggccgaagcc gcgcgcctca cgtcgcacca ccacggtccg gtgatcgcgg caggctcgac
     1261 gggctcgatg ccggcaacgg cgaagttcct tcatgctgtc gccaacctct cgcatggcgc
     1321 ggtggtgctg ccggggctcg ataccgatct tgacgacgac gcctggcatt tgatcggcgg
     1381 cgtcagggat gcgctgggaa cgttcaccac ccatccggcg tccaatcatc cgcaattcgc
     1441 gatgtattcg ctgctgcaac gcttcgggat caaacgcgcc gacatcgaaa ttctcggcgc
     1501 gccctcgccg cggggccgcg atgtgctggt gtccgaggcg atgcggcctt cgaccgcgac
     1561 cgcgcattgg cacgagcggc tgaagcagcc ggagatcgtc gcgaagatcg cggccggcat
     1621 gacggacctt gccgtgatcg aagccgccaa tccggaaatg gaggcgctgg cgatcgccgt
     1681 cgcgatgcgc gaggcgcggc atctcggcaa gtcggcggcg ctggtaacgc cggaccgggc
     1741 gctggcgcgc cgggtgacgg cggcgctcga ccgctggaac ctcgaattcg acgattcggg
     1801 cggcgacgcg ctgatggata cctccgctgg gattttcgcg cggctcgcgg cggaagccgt
     1861 gaccaaaggg cttgaaccgc ctacgctgct ggcgctgtta aaacacccgc tgtgccgaat
     1921 gggcggcgcg gaaggcgcga tatcaggcgc cgtcgagata ctcgaactgg cgctgttgcg
     1981 tggcacccgg ccgcaggccg gaagcgctgg cctggcgcgg gattttgcgc tttttcgcag
     2041 cgaactggcc aaactcaggc gggaagagga atcatcgcta cacaggtcgg agcagagaac
     2101 caggctttcg gttgaagacc tcgatcgcgc gcagtcgctg atcgcgcagt tgcagaaggc
     2161 gctttcgccg ctggaagttc tctcatcgtc aaaaccgtat gatttcgccg aactcgcgga
     2221 gcgccatcgc gagatgctga ccgagctgtc gcgcgacccg catggcgcgg cgctggcatt
     2281 cgagggacag cagggtacgg ggctcgttgc cgcgttcgac gatctcatcg gcgcgcagac
     2341 ccgttccggc ctgatggtcc gactcggcga ctacccggaa gtattccaga ccgcctttgc
     2401 cgaccgcatg gtgcggcggc cggaatccgc caacgcgcaa ctgcacatct acggccagct
     2461 ggaagcccgg ttgacacaat ccgatcgcgt catcctgggc gggctggtcg aaggcgtatg
     2521 gccgccggcg ccgcgaatcg atccgtggct gagccggccg atgcggcacg aactggggct
     2581 cgatctgccg gagcggcgca tcggcctctc cgcgcatgac tttgcgcaac tgcttggttc
     2641 ggacgaagtc attctaagcc acgccgcgaa ggtcggcggc gcgcccgccg tggcctcgcg
     2701 cttcctgcat cgactcgagg cagtggcggg cgaggaccac tggaaagccg cgaaagcagc
     2761 gggcgacaaa tatgtcctgt tcgccaacga actcgaccgt cccgcaaaag tcgtaccgat
     2821 cccgcagccc aggccaaggc cgccgcgcgc agccaggccg ctaaagctgt cggtaaccgc
     2881 catcgaggac tggctgcgcg atccctatac gatctacgcg aagtacattc tgcggttatc
     2941 tccgctcgat cccgtcgaca tgccgctgtc ggccgccgat cgcggctcgg cgatccacga
     3001 cgcgctcggt aaattcacgc agagctattc cacggcgctg cccgacaatc ccgcgctcgc
     3061 cttgcgcgaa atcggcgaaa agtcttttgc accgctgatg gagcggccgg aggcgaaggc
     3121 gctgtggtgg ccgcgattcc agcgtatcgc ggcatggttc gcaggctggg aaacggtacg
     3181 gcgcggggat gtcgtttcga tcgacgccga gacccgcggc aatatctcga ttcgcctcga
     3241 caatgagcgc accttcgagc tgtcggcgcg cgccgaccgg atcgagcgcc gccgtgacgg
     3301 cacctttgcg atcctcgact acaagaccgg gcagccgccg acgggaaagc aggtccggat
     3361 gggattgtcg ccgcaactga cgctggaggc ggcgatcctg cgcgagggcg gcttcaagga
     3421 gatcgcagcg gacagttcgg tcagcgagct ggtttatgtc agactcagcg gcaacaatcc
     3481 gccgggcgag cagcggctgc tggagctcaa gatcaaccag aaagagcagc cgcagtcgcc
     3541 cgacgccgcg gcggacgagg cccgcttgaa gctggaagcg ctgatccgcg ccttcgaaga
     3601 tgaaagccag gcctatacct cgctcaatct accgatgtgg accagtcgct acggcagcta
     3661 cgacgatctc gcgcgcatca aggaatggtc ggcggccggc gggttggaaa tcccggaatg
     3721 gtgaagtccg tgcgccagat ccccgacgcc gtccgcgaca cccagaggcg tgcgtccgac
     3781 ccgacctctt cggcgttcgt ttccgccaat gccggctcgg gcaagaccca tgtgctggtg
     3841 cagcgggtca ttcgcctgct gctggccggc gtgccgcctg aaaaaatcct ctgcatcacc
     3901 ttcaccaagg ccgccgccgc caatatggcg gagcgggtgt tcaccacgct cggacactgg
     3961 atcacgcttg acgacgaggc gctggatgat gcgatcagcg acgtcggcat cgccaatccc
     4021 aacttgaaac tgcggatgcg ggcccgcgaa ttgttcgcct gcgcgctgga gacgcccggc
     4081 ggcctgaagg tgcagaccat ccacgcgctc tgcacaaggc tgctgcaaca atttccgttc
     4141 gaggccaatg taccggcgcg gttcgcggtg ctcgacgacc gcgaccagaa cgaaatgatg
     4201 gagcgcgcca acctcgcggt gctgctggag gcctcgcgca atcccgacag tgcgacggga
     4261 cgcgcactcg acgtcgccat ggccagtgcg gccgacgtca ccttcaagga cgtggtgcgc
     4321 gaggcgtgcc tgagccgcga ccatttcatg gcatggaccg acgatgccgg cagcgccgag
     4381 gtcgcggcgg cgcagatggc ggcggcgctc ggggtcagcg cggatgaccg tatcgaagac
     4441 cttgagcggg agatcaccga cggtccttat ctcagacgct cagagtggga ggagatcgtc
     4501 accgtcctcg agacgggcag caaatccgat caggatcaag cggcgcggct gcgcgcggca
     4561 ctgctggtca caggtagcgc gcaagtggac gcatacatca gcctgttcct caccgacacc
     4621 gagcgcacgc cgcgcaagac cgtggtgacc aaaaagctcg ccgacaacca tccggcggtt
     4681 gcacgcctgt ttgaagccga attccttcgt atcggcccgc tgatcgagcg tcgccgcgcg
     4741 gtgacaaccc gcgaccgcac acaggccctg ttgcacatcg cgaccgcggc agccgcgaat
     4801 taccggcgcg agaagcagga gcgcggcctg ctcgattacg acgacctgat cgacaagacg
     4861 ctggcgatgc tggatcgggt ttcctccggc tgggtgcact acaagctcga tcgcggcgtc
     4921 gatcacgtac tgatcgacga ggcgcaggac accagcccgc ggcaatggga catcgtcgcg
     4981 cacatcatct ccgaattcac ctcgggcgcc ggcgcgcgcg acggcgtggt gcgaacggtg
     5041 ttcgcggtcg gcgacgagaa gcagtcgatc ttttcgttcc agggcgcggc gccccgcgag
     5101 ttcgatctgc gccgccgcgc gttgcagcga aagttcgagg gcgccggact gaagttcgat
     5161 ccggtttcct tcacctattc gttccgctcg gggccggaga tcctgcacgc ggttgatcag
     5221 gtgtttcgcg agcaggatat ctttcgcagc atccatgcgg tcgatatcgg aaatccgatc
     5281 catcacgcgc tggccgacgc cggcccgggc cagatcgacc tctgggaact cgcggaaacc
     5341 gacgaccggc aggacatcga aggctggcgc gcgccgttcg acggcgtcgc ggcgaccagt
     5401 cccgaagtga agcttgcccg gcgcatccag gccgagatca agacgctggt cggcagcggt
     5461 gtgatgaccg gcagcaagaa tgaccgccgt ccgctgcgct atggcgacat gctggtgctg
     5521 gtgcgccggc gcggcaatgc gttcgatgct gtgatccagg cgctgaagca cgccggtatt
     5581 ccggtcgccg gcgccgaccg cctcaagctg accgagcata ttgcgatcat cgacctgatg
     5641 aacctggccg acgcgctgct gctgccgcag gacgatctgg cgctggcggt ggcgctgaag
     5701 agcccgctgt tcgccctcac cgatgacgac ctgttcaaga tcgcctggca gcgcaaggga
     5761 tcgttgcgcg cggcattggc cgagcgcgcc gcggccgacg gccggctgag ggatgcgctg
     5821 tggaggctcg aacaatgcga gcggcgcttt gcaagcgaaa caccgtttgc gttctatgcc
     5881 tggctgctcg gcggcgatgg tggccgcgcg cgaatcctgc ggcggctcgg acacgaggca
     5941 aacgacgcgc tggatgagtt tctggagctg gcgctaaacc acgaacgcaa ggcgccggcc
     6001 tcgttgcagg gttttatggc gtggctacgt acggctgacc ttgaggtgaa gcgcgacatg
     6061 gaaatttcgc gcgacgaggt gcgggtgatg accgtgcacg gcgccaaggg cctggaagca
     6121 tccgtggtga tcatggctga taccacgacc tcgccttcgg acacgcagcg gctgcggctg
     6181 atccatctg
//
LOCUS       DATMJB010000106         4425 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_873877, whole genome shotgun sequence.
ACCESSION   DATMJB010000106 DATMJB010000000
VERSION     DATMJB010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873877"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1309
                     /locus_tag="VNU48_02390"
     CDS             269..1309
                     /locus_tag="VNU48_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HWI10155.1"
                     /translation="MSTPADRAPGAWTGARERSNLWTLRLMRWIAIRAGRPAARLVLH
                     PITLYFLLVNGRARRASMNYLVRVLARPVRWADAYRHIHHFAATILDRVYFLQDRFDA
                     FEVVSTGVEVLDAPLATGEGVMLVGAHLGSFEALRASAQGRGARVAMLMYEDNARLIN
                     ATLAAVAPTAQLHTIALGRPGAMLALRRWLDGGGLAGLLADRTLPGHSRGSAGPPQAS
                     STPSGGGHGEAVAGGHPSRSRTLRFDFLGQPARFSDGPFRLAAMLRRQVVFMAGLYHG
                     ANRYELRFVELADFRPGAECGNLDVDQRVLAAMRAYVALLESLCRETPYNWFNFFDFW
                     ADDDAAAHPAPH"
     gene            1281..1931
                     /locus_tag="VNU48_02395"
     CDS             1281..1931
                     /locus_tag="VNU48_02395"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HWI10156.1"
                     /translation="MPPLTLPRTDRPTRRDALALLVAGAGALAWPARETRAQGTFDLV
                     QLMRTLAQVKAGEATFVEKRSIAMLERTLQSSGRLSFEAPDTFVRETLKPTQEKVAVI
                     GNAVTMSRGTRSRTVPLDSVPEAAVIMEAIRGTLTGNRAAIERNFSASVSGTAQRWEL
                     ELRPNEPRLRELVISVRLSGAQALVREVVVAMADGDRSVMTIEPVVPGARPASAAS"
     gene            1944..4340
                     /locus_tag="VNU48_02400"
     CDS             1944..4340
                     /locus_tag="VNU48_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HWI10157.1"
                     /translation="MPILFARLRDLARRHALALWLACVVVAASIGARTNYVADLSAFL
                     PSTPSAEQAVLLDQLRGGIAARLLLIGIEGGTAPQRSEASLRLANTLRQSGAFDAVHN
                     GDNSGFEATGKFLFEHRYLLSPAVDAQRFTVAGLRAGIDEATSLLGTPAGSAIKAVIL
                     RDPTGETVRMAESMLPSQAPRSDGRVWVSRSAARAVLIATTHADGADLDAQQRTLQLV
                     RTSFAPFEPQGLRLVLSGSGTFAVASRTQIKAEVERLAVAGGVAIVGLMLLAFGGSLK
                     TLATALLPVGSGVLAGIAAVSLIFGNVHGITLGFGTTLIGEAVDYAIYYLIQARPAAG
                     APAGDGARRWLADNWPTVRLGLWTSVCGFAALLFSGFPGLAQLGVFSIAGLSAAALTT
                     RHVLTLTSPDGAPGMGLRRHLGRVVGACTAVLPRLRWPLAAVALGATLTLASLPSPWR
                     GDLGSLSPVGAEKMAIDAELRADLGATDAGVLVAMSASDEAGALAAAEAAGARLDPLV
                     DAGAIAGYESPARMLPSPALQKLRRDALPDAATLRARLAEATVDGPLPAARLGGFVDD
                     VQAARQQALLTRASLKGTPLYAALDALLVPGNGTRPWRALLSLQAGAKAIDPAALRSA
                     LAGVPGAQVVDIGAELRALYARYLHEATLQALLGALAVCVLLALHLRSPARLWRIAQP
                     VAAAVLIVLAGFSALGVALGILHLVGLLLTVAIGSNYALFFDQIRARQDAAPPGTPWV
                     VGNDTLASLALANLTAVISFCLLAFSSIPALFAIGQVVAPGILLSLLLSAAFIPSQAR
                     "
BASE COUNT          545 a         1626 c         1608 g          646 t
ORIGIN      
        1 aactgacttg tcgccgtctg tttgaacgca gcgaacgcag tgagcgaagt gagtttggcg
       61 acgggccacg agaccgagca gcgcagggca ggcggcgcgc agcgacgacc gccgcagtga
      121 agcggagcag cctgcccggg cgcgcctttg ccgcgccgac ggacgcacac caagttcgag
      181 cgtcggcaac gggccgaaag cagagatccg aagccgcagc agcgtttcat gtgtcgtggg
      241 tcgcgcaatg cgcggtggag caactgtcat gagcacgccg gccgatcgtg cgccgggcgc
      301 ctggacgggc gcgcgcgagc gcagcaacct gtggacgctg cgcctgatgc gctggatcgc
      361 gatccgcgcc ggccgccccg ccgcgcggct cgtgctgcac ccgatcacgc tgtacttcct
      421 gctcgtcaac ggccgcgcac ggcgcgcgtc gatgaactac ctggtgcgcg tgctcgcgcg
      481 cccggtgcgc tgggccgatg cctaccgcca catccaccac ttcgcagcga cgattctcga
      541 ccgcgtctac ttcctgcagg accgcttcga tgcgttcgag gtcgtgagca ccggtgtgga
      601 ggtgctcgac gcaccgctgg ccacgggcga aggcgtgatg ctggtgggcg cgcacctggg
      661 cagcttcgag gcgctgcgcg cgagcgcgca gggccgcggc gcgcgcgtcg cgatgctgat
      721 gtacgaagac aacgcacggc tgatcaacgc gacgctcgcg gcggtcgcgc cgaccgcgca
      781 gctgcacacg atcgcgctcg gccgccccgg cgcgatgctg gcgctgcggc gctggctcga
      841 cggcggcggc ctggccgggc tgctggccga ccgcacgctg ccggggcatt cgcgaggaag
      901 cgccgggccg ccccaagctt cctcgacccc ctctgggggc ggacacggcg aagcagtggc
      961 cggggggcac ccatcacgct cgcgcacctt gcgcttcgat ttcctgggcc agccggcacg
     1021 cttctcggat ggcccgttcc gcctcgcggc catgctgcgc cgtcaggtgg tcttcatggc
     1081 cgggctctac catggcgcga atcgctacga gctgcgcttc gtcgagctcg ccgacttccg
     1141 cccgggggcc gagtgcggca atctcgatgt cgaccagcgc gtgctggcgg cgatgcgggc
     1201 gtacgtcgcg ctgctcgaat cgctgtgccg ggaaacgccg tacaactggt tcaacttctt
     1261 cgacttctgg gccgacgacg atgccgccgc tcaccctgcc ccgcactgac cggccgacgc
     1321 gccgcgacgc gctggcgctg ctggtcgcgg gcgccggtgc gctcgcgtgg ccggcgcgcg
     1381 agacacgggc ccagggcacg ttcgacctgg tgcagctgat gcgcacgctc gcgcaggtga
     1441 aggccggcga ggcgaccttc gtcgagaagc gcagcatcgc gatgctcgag cgcacgctgc
     1501 aatcgtcggg gcgcctgagc ttcgaagcgc ccgacacctt cgtgcgcgaa acattgaagc
     1561 cgacgcagga aaaagtcgcg gtcatcggca acgcggtgac gatgagccgc ggcacgcgca
     1621 gccgcacggt gccactcgat tcggtgcccg aggccgcggt catcatggag gcgatccgcg
     1681 gcacgctgac cggcaaccgc gcggcgatcg agcgcaattt ctcggccagc gtcagcggca
     1741 cggcgcagcg ctgggaactc gaactcaggc cgaacgagcc gcggctgcgc gagctcgtga
     1801 tctcggtgcg cctgtcgggc gcacaggcgc tggtgcgtga ggtcgtcgtc gcgatggccg
     1861 acggcgaccg ttcggtgatg acgatcgagc cggtcgttcc cggcgcgcgg ccggcgagcg
     1921 cggcgagctg atccgccgcc gcgatgccga tccttttcgc ccgcctgcgc gaccttgcgc
     1981 ggcgccacgc gctcgcgctg tggctggcgt gtgtcgtggt cgccgccagc atcggcgcgc
     2041 gcacgaacta cgtcgccgac ctgtcggcct tcctgccgtc gacgccgagc gccgagcagg
     2101 cggtgctgct cgaccagctg cgcggcggca tcgccgcacg gttgctgctg atcggcatcg
     2161 agggcggcac ggcgccgcag cgcagcgagg cgtcgctgcg cctggcgaac acgctgcgcc
     2221 aaagcggcgc gttcgacgcg gtccacaacg gcgacaactc cggcttcgag gcgaccggga
     2281 aattcctgtt cgagcaccgc tacctgctga gccccgccgt ggacgcgcaa cgcttcacgg
     2341 tcgccgggct gcgcgccggc atcgacgagg cgacctcgct gctcggcacg ccggccggca
     2401 gcgcgatcaa ggcggtgatc ctgcgcgacc cgaccggcga gacggtccgc atggccgaga
     2461 gcatgctgcc gtcgcaagcc ccgcgcagcg acgggcgcgt gtgggtctcg cgcagcgccg
     2521 cgcgcgcggt gctgatcgcg accacgcacg ccgacggcgc cgacctcgac gcgcagcagc
     2581 gcacgctgca actcgtgcgc acgagcttcg cgccgttcga gccgcaaggc ttgcgactcg
     2641 tcctgtccgg ctcgggcact ttcgcggtcg cgtcgcgcac gcagatcaag gccgaggtcg
     2701 agcgcctcgc ggtcgccggc ggtgtcgcga tcgtcgggct gatgctgctg gccttcggcg
     2761 gctcgctgaa gacgctggcg accgcactgc tgccggtggg ctccggcgtg ctcgccggca
     2821 tcgccgcggt cagcctgatc ttcggcaacg tccacggcat cacgctgggc ttcggcacga
     2881 cgctgatcgg cgaggccgtc gactacgcga tctactacct gatccaggcg cggccggcgg
     2941 ccggtgcgcc ggccggcgac ggtgcgcggc gctggctcgc cgacaactgg cccaccgtgc
     3001 ggctgggcct gtggacctcg gtctgcggct tcgcggcgct gttgttctcg ggcttcccgg
     3061 gcctggcgca gctcggcgtg ttctcgatcg cgggcctgag tgccgcggcg ttgacgaccc
     3121 gccacgtgct caccctgacc tcgcccgacg gcgcgcccgg catggggttg cgccgccacc
     3181 tcggccgcgt cgtcggcgcg tgcaccgcgg tgctgccgcg gctgcgttgg ccgctcgccg
     3241 cggtcgcgct gggggccacg ctgaccctgg cctcgctgcc gtcgccgtgg cgcggcgacc
     3301 tgggctcgct gagcccggtc ggcgccgaga agatggcgat cgatgccgag ctgcgcgccg
     3361 acctcggcgc gaccgacgcg ggcgtgctcg tcgccatgtc ggcgtcggac gaagccggcg
     3421 cgctcgccgc cgccgaggcg gccggtgcgc ggctggaccc gctcgtcgat gccggcgcaa
     3481 tcgccggcta cgaatcgccg gcccgcatgc tgccgagccc ggcgctgcag aagctgcgcc
     3541 gcgacgcgct gcccgatgcg gcgacgctgc gcgcgcgcct ggccgaggcc acggtcgacg
     3601 gcccgctgcc cgcggcccgc ctgggcggct tcgtcgacga cgtgcaggcg gcgcgtcagc
     3661 aagcgctgct gacgcgtgca tcgctgaagg gcacgccgct gtatgccgcg ctcgatgcgc
     3721 tgctcgtgcc cggcaacggc acccgcccgt ggcgcgcgct gctgagcctt caggccggcg
     3781 cgaaggcgat cgatccggcc gcgctgcgca gcgcgctcgc cggcgtgccc ggcgcgcagg
     3841 tcgtggacat cggtgccgaa ctgcgcgcgc tctacgcacg ctacctgcac gaggcgacgc
     3901 tgcaggcgct gctgggcgcg ctcgcggtgt gcgtgctgct ggcgctgcac ctgcgctcgc
     3961 cggcgcggct gtggcgcatc gcgcagccgg tcgccgcggc ggtgctgatc gtgctcgcgg
     4021 ggttcagcgc gctcggcgtg gcgctgggca tcctgcacct cgtcgggctg ttgctgacgg
     4081 tggcgatcgg ctccaactac gcgctgttct tcgaccagat ccgggcccgg caggacgcgg
     4141 ccccgcccgg cacgccctgg gtcgtcggca acgacacgct ggcctcgttg gcactggcga
     4201 acctgaccgc ggtgatctcg ttctgcctgc tcgccttctc gagcatcccg gcgctgttcg
     4261 cgatcggcca ggtcgtggcg ccgggcatcc tgctgagcct gctgctgtcg gccgcgttca
     4321 tcccgtcaca ggcgcgctga acggcgtgtg agaatccttc ggcccgtgcc tccccacccc
     4381 acacgcgacg ccatgcacgc cccgtcttcc ccggctccca cgccc
//
LOCUS       DATMJB010000107         4292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_459257, whole genome shotgun sequence.
ACCESSION   DATMJB010000107 DATMJB010000000
VERSION     DATMJB010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459257"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /gene="ccoN"
                     /locus_tag="VNU48_02405"
     CDS             complement(<1..1303)
                     /gene="ccoN"
                     /locus_tag="VNU48_02405"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HWI10158.1"
                     /translation="MASSVPAPAAAAGPVYDDSAVRLFTLAAVVWGVVGMLVGVVIAS
                     QLTWPELNFGIPWLSYGRLRPLHTNAVIFAFGGCALMATSFYVVQRTSQVALFLPKLA
                     AFVFWGWQTVIVAAVITLPLGITQGKEYAELEWPIDLLLTVLWVAYAIVFFGTIGIRK
                     VRHIYVANWFFGAFIIAVALLHIVNSAAVPVGLWKSYSAYAGVQDAMVQWWYGHNAVG
                     FFLTAGFLGMMYYFIPKQAERPIYSYRLSIVHFWALIFTYMWAGPHHLHYTALPDWAQ
                     TLGMVFSIMLWMPSWGGMINGLMTLSGAWDKLRTDPGLRFSVTAVAFYGMATFEGPVM
                     SIKSVNGLSHYTDWTIGHVHSGALGWVGFIVFGSLYYLVPVMWGRRRMYSERLIAWHF
                     WIATIGIVLYITSMWVAGIMQGLMWRAYDQLGFLQYSFAETV"
     gene            complement(1358..1528)
                     /gene="ccoS"
                     /locus_tag="VNU48_02410"
     CDS             complement(1358..1528)
                     /gene="ccoS"
                     /locus_tag="VNU48_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HWI10159.1"
                     /translation="MDILFLLVPASVLLVFGLLALFRWALGAGQFDDLEREGERILDD
                     TASPLDAHQGRD"
     gene            complement(1532..3805)
                     /locus_tag="VNU48_02415"
     CDS             complement(1532..3805)
                     /locus_tag="VNU48_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HWI10160.1"
                     /translation="MTAPIASARPIGATLDDPHAQAGFTSWATAADGERVARSHFRLT
                     GLYCAACAGPIEAALRAEPGVIDAEVSYATRRASVRWRPAATRPTLLVAAVARAGYGA
                     APDLAEPAQALRRAEQRAALWRLFVAAFCMMQVMMYAAPAYVAAPGTIAPDLLRLLQW
                     ASWLLSIPVLVFSAAPIFREAWAGLSAGQARMDLPVAIGLAVTFVASSGATFAPGGAF
                     GREVYFDSLTMFVCFLLAGRWLSLRAQHRVAAALEDACARLPSDVRRVADDGSFEMAP
                     LRSVMVGDRLRVFAGEAFAADGLLIDGRTRVDEALLTGESRPLARAPGERVIAGSLNL
                     GAPVTLRVERVGPDTRFEGIVALMRGALTQRPALTRSADRIALPFLWGVLALAALAGA
                     AWSVIDPSRAVWVVVSVLIVTCPCALSLSAPSALLAAAGALARRGVLVRRLDALEALA
                     GIDTLFFDKTGTLTEGGMRLRRVGLLPAAHAAGLDGAVVLRLAGALARHSSHPLSQAL
                     ACEVSVPEAVWSEVTETPGAGVQGAAWDGSPARLGAHAWVDATGLVPADAGAAQVWFG
                     DARGALAWFEFDETLKPDARASLEDLRGDGVALALLSGDAPLRVRAVGLALQLPDAQG
                     GATPELKLERVAAVQRAGHRVGMVGDGLNDAPVVARADVSFVFAQGAPITQSSADFIL
                     LSGRVGDVALARRVARRAMRVLRQNFAWAIAYNAVCVPLALLGWFPPWAAGIGMAASS
                     LVVVLNALRIDARALKG"
     gene            3969..>4292
                     /locus_tag="VNU48_02420"
     CDS             3969..>4292
                     /locus_tag="VNU48_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HWI10161.1"
                     /translation="MDHTPTRKPFYKSLYLQVITAIVIGVLLGHFYPSTGEAMKPLGD
                     AFIKLIKMIIAPIIFCTVVVGIAGMEDMKKVGKTGGLALLYFEIVSSVALIVGLVVIN
                     LVQPGA"
BASE COUNT          740 a         1564 c         1479 g          509 t
ORIGIN      
        1 ccacggtctc ggcgaacgag tattgcagga agccgagctg atcgtaggcg cgccacatca
       61 gcccctgcat gatgccggcg acccacatcg aggtgatgta gagaacgatg ccgatcgtcg
      121 cgatccagaa gtgccacgcg atcagccgct ccgagtacat ccgccgccga ccccacatca
      181 ccggcaccag gtagtagagc gagccgaaga cgatgaagcc gacccagccc agcgcgccgg
      241 aatgcacgtg gccgatcgtc cagtcggtgt agtggctgag gccgttgacc gacttgatcg
      301 acatcaccgg cccctcgaag gtcgccatcc cgtagaacgc gaccgcggtg accgagaagc
      361 gcagacccgg atcggtgcgc agcttgtccc atgcgcccga cagggtcatg agtccgttga
      421 tcatcccgcc ccagctcggc atccacagca tgatcgagaa caccatgccg agcgtctgcg
      481 cccagtcggg cagcgcggtg tagtgcaggt ggtgcgggcc ggcccacatg taggtgaaga
      541 tcagcgccca gaagtgcacg atcgacaacc ggtacgagta gatggggcgc tcggcctgct
      601 tcgggatgaa gtagtacatc atcccgagga agcccgcggt caggaagaag ccgaccgcgt
      661 tgtgaccgta ccaccactgg accatcgcgt cctgcacgcc ggcgtaggcc gagtagctct
      721 tccacaggcc gacgggaacg gccgcgctgt tgacgatgtg cagcagcgcc acggcgatga
      781 tgaacgcgcc gaagaaccag ttggcgacgt agatgtggcg caccttgcgg atgccgatgg
      841 tgccgaagaa cacgatcgcg taggcgaccc agaggacggt gagcagcagg tcgatcggcc
      901 attcgagttc ggcgtactcc ttgccctgcg tgatgcccag cggcagcgtg atcaccgccg
      961 cgacgatcac ggtctgccag ccccagaaca cgaaggccgc cagcttcggc aggaacagcg
     1021 cgacctgcga ggtgcgctgc acgacgtaga agctggtggc catcagcgcg cagccgccga
     1081 acgcgaagat caccgcgttg gtgtgcagcg gccgcagccg cccgtagctc agccacggga
     1141 tgccgaaatt cagttccggc caggtgagtt gcgacgcgat gaccacgccc accagcatgc
     1201 ccacgacgcc ccacaccacc gcggccagcg tgaacagacg caccgcgctg tcgtcgtaga
     1261 ccgggcctgc cgcggccgca ggggctggaa ccgaactcgc catctcgctt ctcctcattg
     1321 accgcaagcg cggcagggag aagtgtcgat tcgttcgtca gtcgcgacct tgatgcgcat
     1381 caagcggcga cgctgtgtcg tccaggatgc gctcgccttc gcgctccagg tcgtcgaact
     1441 gacccgcgcc cagcgcccag cggaacagcg cgagcaggcc gaacacgagc agcacggagg
     1501 ccgggaccag caggaacagg atgtccatcg atcaaccctt cagcgcgcgc gcgtcgatgc
     1561 gcagcgcgtt cagcaccacc accagcgaac tcgccgccat gccgatcccc gcggcccagg
     1621 gcgggaacca ccccagcagc gccagcggca cgcacacggc gttgtaggcg atcgcccagg
     1681 cgaagttctg gcgcagcacg cgcatcgcgc ggcgtgcgac gcggcgtgcc agcgcgacat
     1741 cgccgacgcg ccccgacagc aggatgaagt ccgcgctcga ctgcgtgatc ggcgcgccct
     1801 gcgcgaacac gaacgacacg tcggcgcggg cgacgaccgg cgcgtcgttc aagccgtcgc
     1861 cgaccatgcc gacgcggtgg ccggcgcgct gcaccgcggc cacgcgttcg agcttcagct
     1921 cgggcgtggc gccgccctgc gcgtcgggca gctgcagcgc caggccgacc gcacgcacgc
     1981 gcagcggcgc gtcgcccgac agcagcgcga gcgccacgcc gtcgccgcgc agatcctcga
     2041 gcgaggcgcg cgcgtcgggc ttgagcgtct cgtcgaactc gaaccaggcg agcgcaccac
     2101 gcgcgtcgcc gaaccagacc tgggccgcgc cggcatcggc cggcacgagg ccggtcgcat
     2161 cgacccatgc gtgcgcaccc aggcgcgccg gcgagccgtc ccaggccgcg ccctgcacgc
     2221 cagcgccggg cgtctcggtg acctcgctcc agacggcctc gggcacgctg acctcgcacg
     2281 ccagcgcctg cgacagcggg tgcgacgaat gtcgtgccag cgcgccggcc aggcgcagca
     2341 cgaccgcacc gtccaggccc gcggcgtggg ccgcgggcag cagaccgacg cggcgcaacc
     2401 gcatgccgcc ctcggtcagc gtgccggtct tgtcgaagaa cagcgtgtcg atgccggcca
     2461 gcgcctcgag cgcgtcgaga cgccgcacca gcacgccgcg ccgggccagc gcgccggccg
     2521 cggccagcag cgccgacggc gcggacagcg acagcgcgca cgggcaggtg acgatcagca
     2581 ccgacaccac gacccagacc gcgcgcgacg ggtcgatcac cgaccaggcc gcgccggcca
     2641 gggccgcgag cgcgagcacg ccccacagga acggcaaggc gatccggtcg gcgctgcgcg
     2701 tcagcgccgg gcgctgcgtc agcgcgccgc gcatcagcgc gacgatgcct tcgaagcgcg
     2761 tgtccgggcc gacccgctcg acgcgcagcg tcaccggcgc gcccaggttc aggctgcccg
     2821 cgatgacgcg ctcgccgggc gcgcgcgcca gcgggcgcga ctcgccggtc agcagcgcct
     2881 cgtcgacccg cgtgcgaccg tcgatcagca ggccgtcggc cgcgaaggcc tcgccggcga
     2941 acacccgcaa gcggtcaccg accatcacgc tgcgcagcgg cgccatttcg aacgagccat
     3001 cgtcggcgac acgccgcacg tcggacggca gccgcgcgca ggcgtcctcg agcgccgccg
     3061 cgacgcggtg ctgcgcccgc aggctcaacc agcggccggc gagcaggaag cacacgaaca
     3121 tcgtcagcga gtcgaaatag acctcgcggc cgaacgctcc gcccggcgcg aaggtcgcgc
     3181 cgctgctggc gacgaaggtg acggccaggc cgatggccac cggcaggtcc atgcgcgcct
     3241 ggccggcgct gaggccggcc caggcctcgc ggaagatcgg cgcggccgag aacaccagca
     3301 ccgggatgct gagcagccac gaggcccatt gcagcaggcg cagaaggtcc ggcgcgatgg
     3361 tgccgggcgc ggcgacgtag gccggcgccg cgtacatcat cacctgcatc atgcagaagg
     3421 cggcgacgaa caggcgccac agcgcggcgc gctgctcggc gcgacgcaag gcctgcgccg
     3481 gctcggccag gtcgggcgcg gcgccgtagc cggccctcgc gaccgccgcg accagcagcg
     3541 tcgggcgcgt cgccgccggg cgccagcgca ccgaggcgcg gcgcgtggca tagctgacct
     3601 cggcatcgat cacgcccggc tcggcccgca aggcagcctc gatcgggccg gcgcaggccg
     3661 cacagtacag gccggtcagg cgaaagtgcg agcgcgccac gcgctcgccg tcggcggcgg
     3721 tcgcccacga ggtgaagccg gcctgcgcgt gcgggtcgtc gagcgtggcg ccgatggggc
     3781 gtgcggaggc gatcggcgca gtcatggcgc aatctacgac ggcgccgccg cgcgccgctt
     3841 gcgcttcgtc aagcgcggcg gcgcggcctc aattcgctcg cgtctgtgga atccctcttg
     3901 cgcccggtcg ccggccgcgg taacactgcg gctcgcgtgc cataccaaga atttccgcga
     3961 tcgcgcccat ggaccacacc cccacccgca agccgttcta caagtccttg tacctgcagg
     4021 tgatcaccgc gatcgtcatc ggcgtgttgc tggggcactt ctacccgagc accggcgagg
     4081 cgatgaagcc actcggcgac gccttcatca agctgatcaa gatgatcatc gcgccgatca
     4141 tcttctgcac cgtggtggtg ggcatcgccg gcatggagga catgaagaag gtcggcaaga
     4201 ccggcggcct ggcgcttctc tacttcgaga tcgtcagcag cgtggcgctg atcgtgggcc
     4261 tggtcgtgat caacctggtg cagccgggcg cc
//
LOCUS       DATMJB010000108         4519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_885946, whole genome shotgun sequence.
ACCESSION   DATMJB010000108 DATMJB010000000
VERSION     DATMJB010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885946"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..809
                     /locus_tag="VNU48_02425"
     CDS             147..809
                     /locus_tag="VNU48_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HWI10162.1"
                     /translation="MKKYLIAGLLVWLPLAITIWVLQAALGLLDGVFGWFLNAARAVL
                     PAAAEPLIDLLLKIPGLGVIVMLSALLLTGVFATNMVGQWWLRQGSRALSKIPIVKSI
                     YNSVKQVSDTLFSSSGNAFREAVLVQYPRAGSWTIAFVTGKPGGEAALHLSGDYLSVY
                     VPTTPNPTSGFFLMVPRADVVVLQMSVDEALKYVISMGVVAPPLHVVTNPAVAGVEPA
                     RN"
     gene            949..2742
                     /gene="aspS"
                     /locus_tag="VNU48_02430"
     CDS             949..2742
                     /gene="aspS"
                     /locus_tag="VNU48_02430"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HWI10163.1"
                     /translation="MRTTYCGLVSEALMGQTVTLMGWAHRRRDHGGVIFIDLRDREGI
                     VQVVCDPDRAEMFRVAEEVRNEFCLKIIGKVRARPDGTVNANITSGKIEVLCLEIEVL
                     NPSITPPFQLDDDNLSETTRLTHRVLDLRRPAMQRNMMLRYRVAMEVRKFLDANGFID
                     IETPMLTKSTPEGARDYLVPSRVHDGMFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDIETSFLTEEEIRTMFEGMIRSTFQHAIGVELPAFPVMKYADA
                     MHRYGSDKPDLRVKLEFTELTDVMAGVDFKVFSTPATTQGGRVVALRIPGGGEMSRGE
                     IDAYTEFVKIYGAKGLAWIKVNDVAKGREGLQSPVVKNLHDAALAAILERSGAKSGDL
                     LFFGADKAKVVNDAIGALRVKVGHSEFGKARGLVQGVWEPLWVVDFPMFEYDDGAQRW
                     SAVHHPFTSPKDGHEDLLETDPGAAVSKAYDVVLNGIELGGGSVRIHREEVQSKVFRA
                     LKIDAEEAQLKFGFLLDALQYGAPPHGGIAIGLDRFVMLMTGAESLRDVIAFPKTQRA
                     QDLLTNAPSAVDEKQLRELHIRLRNVQGTGG"
     gene            2824..3288
                     /gene="nudB"
                     /locus_tag="VNU48_02435"
     CDS             2824..3288
                     /gene="nudB"
                     /locus_tag="VNU48_02435"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HWI10164.1"
                     /translation="MTKLPKIPESVLVVIHTVDLDVLLIERADHPGYWQSVTGSKDTP
                     DEPPAVTAQREVFEETGLLIGSPEVPLAGLRDWQLRNVYEVYPVWRHRYAPGVTHNTE
                     FVFGLRVPRGVPVTLSPREHTAHVWLPWREAADRCFSPSNAEAILQLPRFVA"
     gene            3293..4096
                     /locus_tag="VNU48_02440"
     CDS             3293..4096
                     /locus_tag="VNU48_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWI10165.1"
                     /translation="MRASSTTQVHSTQHPSHILRVATYNIHKGVRGVGPRKRLEIHNL
                     ALGIEALDTDLVFLQEVRFLNRAEAKKFPDTLIGWPRMPQADYLAPEGFEVAYRTNAT
                     TTHGEHGNALLSRWPLGDIGHHDVSDHRFEQRGLLHVPVHWNGVLVHAIVAHFGLMHA
                     SRVRQVERLAKFIEAEVPKGELLIVAGDFNDWGAKLDAPMRAFGLQRAGVPGDPPARF
                     NTFPSRLPVLSMDRIYLRGLQCLSTSVPRGAAWARMSDHLPLIAELTLE"
     gene            4093..>4519
                     /locus_tag="VNU48_02445"
     CDS             4093..>4519
                     /locus_tag="VNU48_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWI10166.1"
                     /translation="MNAPIRTRALRPDHNLTLLKGGEALFPALIEAIANARAEVLLET
                     YMFEFARSALTVAQALEAAAQRGVCVRVIVDGVGTGDVPAEWQRRWRAAGAHWRVFNP
                     AQGWRVLLPKRWRRLHRKLCVVDGSIGFCGGINLLDDYFD"
BASE COUNT          723 a         1584 c         1535 g          677 t
ORIGIN      
        1 cgagccggcg aagggcgcgg ctgcggcggc gaccgagaag cccgccggcg ccaagaccga
       61 cgctgcgccg gcacccgcgc cggccgcagc gccggcggcg cccgcttctg gcggcaaatg
      121 agcgtgcggg ttcgccgggg aacgccgtga agaaatacct catcgccggg ctgctggtct
      181 ggctgccgct tgcgatcacg atctgggtgc tgcaggccgc actcggcctg ctcgacggcg
      241 tgttcggctg gtttctgaac gccgcccgcg cggtgctgcc ggcggccgct gagccgctga
      301 tcgacctgct gctgaagatc ccgggcctgg gtgtgatcgt gatgctgtcg gctctgctgc
      361 tgaccggcgt gttcgcgacc aacatggtcg gccagtggtg gctgcgccag ggcagccggg
      421 cgctgagcaa gatcccgatc gtcaagtcga tctacaactc ggtcaagcag gtttccgaca
      481 ccctgttctc gagcagcggc aacgccttcc gcgaagcggt gctggtgcag tacccgcgtg
      541 ccggcagctg gaccatcgcg ttcgtcaccg gcaagcccgg cggcgaggcc gcgctgcacc
      601 tgagcggcga ctacctgagc gtgtacgtgc cgaccacgcc gaacccgacc tcgggcttct
      661 tcctgatggt gccgcgcgcc gacgtggtgg tgctgcagat gagcgtcgac gaggccctga
      721 agtacgtgat ctcgatgggc gtggtggcgc cgccgctgca tgtggtcacc aacccggcgg
      781 tcgccggcgt cgagccggcg cgaaattgac cgggctgggc ggcccggctt ctgcccagcc
      841 cgagcaggcg cgcccagccg cgccgcagag ctcgcgaacc gaacgacgcg cccacgtttc
      901 ggcgcgtcca cttccaaacc aatctgcccc gagcagtgga gtgatgtgat gagaacaaca
      961 tactgcggcc tggtcagcga agcgctgatg ggccagaccg tgacgctgat gggctgggcg
     1021 catcgccgcc gcgaccacgg cggcgtcatc ttcatcgacc tgcgcgaccg cgaaggcatc
     1081 gtccaggtcg tctgcgaccc ggaccgggcc gagatgttca gggtcgccga agaggtgcgc
     1141 aacgagttct gcctgaagat catcggcaag gtgcgcgccc gccccgacgg cacggtcaac
     1201 gccaacatca ccagcggcaa gatcgaggtg ctgtgcctcg agatcgaggt gctgaacccg
     1261 agcatcacgc cgccgttcca gctcgatgac gacaacctgt cggagacgac gcgcctcacg
     1321 caccgcgtgc tcgacctgcg ccgcccggcg atgcagcgca acatgatgct gcgctaccgc
     1381 gtcgcgatgg aggtgcgcaa gttcctcgac gcgaacggct tcatcgacat cgagacgccg
     1441 atgctcacca agagcacgcc cgaaggcgcg cgcgactacc tcgtgccgag ccgcgtgcac
     1501 gacggcatgt tcttcgcact gccgcagagc ccgcagctct tcaagcagct gctgatggtg
     1561 gcgggcttcg accgctacta ccagatcacc aagtgcttcc gcgacgaaga cctgcgcgcc
     1621 gaccgccagc ccgagttcac gcagatcgac atcgagacct cgttcctgac cgaggaagag
     1681 atccgcacga tgttcgaggg catgatccgc agcaccttcc agcacgcgat cggcgtcgag
     1741 ctgccggcgt ttccggtgat gaagtacgcc gacgcgatgc accgctacgg ctccgacaag
     1801 cccgacctgc gcgtcaagct cgagttcacc gagctgaccg acgtgatggc gggcgtcgac
     1861 ttcaaggtct tctcgacccc cgcgacgacc cagggcggcc gcgtggtcgc gctgcgcatc
     1921 cccggcggcg gcgagatgag ccgcggcgag atcgacgcct acaccgagtt cgtgaagatc
     1981 tacggcgcca agggcctggc ctggatcaag gtcaacgacg tcgccaaggg ccgcgaaggc
     2041 ctgcagtcgc cggtcgtcaa gaacctgcac gacgccgcgc tggccgcgat cctcgagcgc
     2101 agcggcgcga agagcggcga cctgctgttc ttcggcgccg acaaggccaa ggtcgtcaac
     2161 gacgcgatcg gcgcgctgcg cgtgaaggtc ggccacagcg agttcggcaa ggcgcgcggc
     2221 ctggtgcagg gcgtgtggga gccgctgtgg gtcgtcgact tcccgatgtt cgagtacgac
     2281 gacggcgcgc agcgctggtc ggccgtgcac cacccgttca cctcgccgaa ggacggtcac
     2341 gaagacctgc tcgagaccga ccccggcgcg gccgtctcga aggcctacga cgtcgtgctc
     2401 aacggcatcg aactcggcgg cggctcggtg cgtatccacc gcgaagaggt gcagagcaag
     2461 gtgttccgcg cgctcaagat cgacgccgag gaggcgcagc tcaagttcgg cttcctgctc
     2521 gatgcgctgc agtacggcgc gccgccgcac ggcggcatcg cgatcggcct ggaccgtttc
     2581 gtgatgctga tgaccggcgc cgaatcgctg cgcgacgtga tcgcattccc gaagacgcag
     2641 cgcgcgcagg acctgctgac gaacgcgccc agcgcggtcg acgagaagca gctgcgcgag
     2701 ttgcacatcc gcttgcgcaa cgtgcagggc acgggcggat gaaggccccg cacgggtgct
     2761 gatgcagaat cgaagggcgc gattttcgcg cccttttttc tgccgcccac gactgccgct
     2821 tccgtgacaa agctgcccaa gattcccgag tcggtgctgg tcgtgatcca cacggtcgac
     2881 ctcgacgtgc tgctgatcga gcgtgccgac caccccggct actggcagag cgtcaccggc
     2941 tcgaaggaca cgccggacga gccgcccgcc gtcaccgcgc agcgcgaggt cttcgaggag
     3001 accggcctgc tgatcggctc gcccgaggta ccgctggccg gcctgcgcga ctggcagctg
     3061 cgcaatgtct acgaggtcta cccggtctgg cgacatcgct atgctcccgg cgtgacccac
     3121 aacaccgagt tcgtcttcgg cctgcgcgtg ccgcgcggcg tgcccgtcac gctgagcccg
     3181 cgcgagcaca ccgcccacgt ctggctgccg tggcgcgagg ccgccgaccg ctgcttctcg
     3241 ccgtcgaacg ccgaggcgat cctgcagctg cctcgttttg tcgcctgagc cgatgcgcgc
     3301 ttcatccacc acccaggtgc acagcacgca gcacccgagc cacatcctgc gggtggcgac
     3361 ctacaacatc cacaagggcg tgcgcggcgt gggcccgaga aagcgcctgg agatccacaa
     3421 cctcgcgctg ggcatcgagg cgctcgacac cgacctggtg ttcctgcagg aagtgcgctt
     3481 cctgaaccgc gccgaggcga agaagttccc ggacacgctg atcggctggc cgcgcatgcc
     3541 gcaggccgac tacctggcgc ccgagggctt cgaggtcgcc tatcgcacga acgcgacgac
     3601 gacgcacggc gagcacggca atgcgctgct gtcgcgctgg ccgctgggcg acatcggcca
     3661 ccacgacgtc tccgaccacc gcttcgagca gcgcggcctg ctgcacgtgc cggtgcactg
     3721 gaacggcgtg ctggtgcatg cgatcgtcgc gcacttcggg ctgatgcatg caagccgcgt
     3781 gcgccaggtc gagcggctcg cgaagttcat cgaggccgag gtgccgaagg gcgaactgct
     3841 gatcgtcgcc ggcgacttca acgactgggg tgccaagctc gacgcgccga tgcgcgcgtt
     3901 cggcctgcag cgcgccggcg tgcccggcga cccaccggcg cgcttcaaca ccttcccgtc
     3961 gcggctgccg gtgctgtcga tggaccgcat ctacctgcgc gggctgcaat gcctgtcgac
     4021 ctcggtgccg cgcggcgccg cctgggcgcg catgtccgac cacctgccgc tgatcgccga
     4081 actgacgctc gaatgaacgc gccgatccgc acgcgcgcgc tgcggcccga ccacaacctg
     4141 acgctgctca agggcggcga agccctgttc cccgcactga tcgaggcgat cgccaacgcg
     4201 cgcgccgagg tgctgctcga gacctacatg ttcgagttcg cgcgctcggc gctgacggtc
     4261 gcgcaggcgc tcgaggccgc ggcgcagcgc ggcgtctgcg tgcgcgtgat cgtcgacggc
     4321 gtgggcaccg gcgacgtgcc ggccgagtgg cagcggcgct ggcgggcggc cggtgcgcac
     4381 tggcgtgtct tcaacccggc gcagggctgg cgcgtgctgc tgcccaaacg ctggcgccgc
     4441 ctgcaccgca agctgtgcgt ggtcgacggc agcatcggct tttgcggcgg catcaacctg
     4501 ctcgacgact atttcgacc
//
LOCUS       DATMJB010000109         2141 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_103669, whole genome shotgun sequence.
ACCESSION   DATMJB010000109 DATMJB010000000
VERSION     DATMJB010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103669"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1263
                     /locus_tag="VNU48_02450"
     CDS             211..1263
                     /locus_tag="VNU48_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI10167.1"
                     /translation="MSTVLLSPQLTPSMTRSTGVWSAAATRFRSDHVGMTSLVIVVLF
                     LLLVLASGVGLVAKDWQVERGVPDAPPTFVGPAAATDSVAVRPPSGPNVDISAVDPLA
                     PRYKEWAEAAKKYQITESVKAQTLPFGGDRLGRDVLAKAIKGAQVSILVGVSSALLAT
                     IIGTLLGAIAGFFGGKAGDLLEWVYNVFTAVPGILLIFAFAAVFGRGIGSVVLILGLA
                     GWTGIYRLVRAEFMKHAVRDYVRSAEAIGASTGSRMFRHILPNVSHVVLVQLSIHVVG
                     FIKSEVILSYLGVGVGVDQVSWGTMLSEAQSELILGHWWQLAAATLFMAVFVTAFSLM
                     TDALRDALDPKLRGLE"
     gene            1260..>2141
                     /locus_tag="VNU48_02455"
     CDS             1260..>2141
                     /locus_tag="VNU48_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI10168.1"
                     /translation="MSTLLSIKDLRVAFRLGKTNGVVQRFEAVKGVSFDVPENSTVAL
                     VGESGSGKSVTAMSILNLLPDNAERSGAITFQGRDMLAAPMSELQKLRGREIACVFQD
                     PMTSLNPVFTVGQQICEPLMKHLGLNARDAMTRAEALLVEVGMPEPKRRLSVYPHQLS
                     GGQQQRVMIAMALACEPKLLIADEPTTALDVTIQRQIMELLAGLKARYRMSMLFISHD
                     LGVVGEIADHVVVMRHGIVREQGAVAGIFAHPQDAYTKALLACRPTLEQRAPRLMVID
                     EHIAAQSQLVAAHTAAGG"
BASE COUNT          324 a          730 c          754 g          333 t
ORIGIN      
        1 gcaacgcagc gaagtaaagg aggagggacg ggcgcagccc gttccggggg acatgagcgg
       61 agttgtccac ccgacgctct cggctcgcgc cgaccgtcaa agggcgcacg ctcgcttgcg
      121 aacgaccgca acgggccgca accagacatc ccaagccacc gcagcgcttc atgcgtcgcg
      181 ggtcgcgcag cgcgcggtgg agcaactgaa atgagcacgg tcctgctcag cccgcagctc
      241 acgccgagca tgacgcgctc gaccggcgtg tggtcggccg cggcgacgcg ctttcgcagc
      301 gaccacgtcg gcatgacttc gctggtcatc gtcgtgctgt tcctgctgct ggtgctggcc
      361 tcgggcgtcg gcctcgtcgc caaagactgg caggtcgagc gcggcgtgcc cgacgcgccg
      421 ccgaccttcg tcggcccggc cgcggccacc gacagcgtcg ctgttcgccc gccgagcggc
      481 ccgaacgtcg acatctcggc ggtcgacccg ctcgcgccgc gctacaagga gtgggccgag
      541 gccgcgaaga agtaccagat caccgaaagc gtgaaggcgc agaccctgcc gttcggcggc
      601 gaccggctcg gccgcgacgt gctcgcgaag gcgatcaagg gtgcgcaggt gtcgatcctg
      661 gtcggcgtct cgtcggcgct gctggccacg atcatcggca ccttgctcgg cgccatcgcc
      721 ggcttcttcg gcggcaaggc cggcgacctg ctcgagtggg tctacaacgt cttcaccgcg
      781 gtgccgggca ttctgctgat cttcgcgttc gcggcggtgt tcgggcgcgg catcggctcg
      841 gtcgtgctga tcctgggcct ggcgggctgg accggcatct accggctggt gcgcgccgag
      901 ttcatgaagc acgcggtgcg cgactacgtg cgctcggccg aggcgatcgg cgcgagcacg
      961 ggttcgcgca tgttccgcca catcctgccc aacgtcagcc acgtggtgct ggtgcagttg
     1021 tcgatccacg tcgtcggctt catcaagtcg gaggtgatcc tgtcgtacct tggcgtcggc
     1081 gtcggcgtcg atcaggtctc gtggggcacg atgctgtcgg aggcgcagag cgagctgatc
     1141 ctcggccact ggtggcagct cgccgcggcg acgctgttca tggcggtgtt cgtcaccgcg
     1201 ttctcgctga tgaccgatgc gttgcgcgat gcgctcgacc cgaaactgcg cggcctggaa
     1261 tgagcacgct gctgtccatc aaagacctgc gggtcgcctt ccggctcggc aagacgaacg
     1321 gcgtggtgca gcgcttcgag gccgtcaagg gcgtgagctt cgacgtgccc gagaacagca
     1381 ccgtcgcgct ggtcggcgaa tcgggctcgg gcaagagcgt gaccgcgatg tcgatcctga
     1441 acctgctgcc cgacaacgcc gaacgctcgg gcgcgatcac gttccagggc cgcgacatgc
     1501 tggccgcacc gatgagcgag ctgcagaagc tgcgcggccg cgagatcgcc tgcgtgtttc
     1561 aggacccgat gacctcgctg aacccggtgt tcacggtcgg ccagcagatc tgcgagccgc
     1621 tgatgaagca cctgggcctg aacgcgcgcg acgcgatgac gcgcgccgag gcgctgctcg
     1681 tcgaagtcgg catgcccgag ccgaagcggc gtctgtcggt ctacccgcac cagctctcgg
     1741 gcggccagca gcagcgcgtg atgatcgcga tggcgctggc ctgcgagccc aagctgctga
     1801 tcgccgacga gccgaccacg gcgctggacg tgacgatcca gcgccagatc atggagctgc
     1861 tcgccgggct gaaggcgcgc taccggatga gcatgctgtt catcagccac gacctcggcg
     1921 tggtcggcga gatcgccgac cacgtggtcg tgatgcgcca cggcatcgtg cgcgaacagg
     1981 gcgcggtcgc cggcatcttc gcgcacccgc aggacgccta cacgaaggcg ctgctcgcct
     2041 gccggccgac gctcgagcag cgcgcgccgc gcctgatggt gatcgacgag cacatcgcgg
     2101 cgcagagcca gctcgtcgcg gcgcacaccg cggccggcgg c
//
LOCUS       DATMJB010000110         4331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_402230, whole genome shotgun sequence.
ACCESSION   DATMJB010000110 DATMJB010000000
VERSION     DATMJB010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402230"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..2061)
                     /locus_tag="VNU48_02460"
     CDS             complement(109..2061)
                     /locus_tag="VNU48_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10169.1"
                     /translation="MRAVSTLLVTGTALWIAAMPAHAVGFGRVVNATQLGQPLDFLAA
                     IRLDADEQLAPECVFAEVQSGENRLAPSLVRVSLEGPVDSNNRGVRVTTGTFIDEPVV
                     TVSVTLGCVAKITRRFVAFVDPPLINAPQTAAAEASPAPPRVEAAVAASAPAAAAGPG
                     AETSIITARPVPAASAARRSARAAASRARSVASAREAITPQAAKPRPISRQPAVARAP
                     TAGPRLHLETAAPIVARGASAAAPAAAPLVADAAASAVKPDPVADPLAAERQRIQVLE
                     EGLAKLTKSAQANQASLIAMQARVQDAESARYANPLVYALAALSALLALAVVWLGWRQ
                     SRARQSGQWWAGPAPQPAPSQTPLAAGAAAAMAKAELEEPPAPELAAAAAPATDVFTV
                     PTVVEDEGSHTSGPARELSVEELIDLEQQVEFFIVLGQDEAAIDLLMSHVRSDGGNSP
                     LPYLKLLEIYRRRGEREAYQRVRERFNRRFNAYAPDWESDPQQGRSLVDYPDTIVRLQ
                     GLWSSPARMMETLEASLFRRNKSDETFDLPAYRELLFLYSIARDLAEHGGALPSAGVD
                     LLLPLDGAGEEPISRLSATSLSADLPNGGLRTTRLDFDLPSDSAAQNAKTSPLGQADR
                     PKLDAPHLIDFNLDAPPEPPKSGTYH"
     gene            2258..3037
                     /locus_tag="VNU48_02465"
     CDS             2258..3037
                     /locus_tag="VNU48_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HWI10170.1"
                     /translation="MSAELLTERRGAALLLTLSDPATRNALSHAVIAAGIEALNMAEA
                     DSELRCVLLRGAGGHFCAGGNLQGLLERRRSGPPAQVRMLEHLHQFIEALRVFPKPVI
                     AAVEGAAAGAGFSLALACDLIVAAEDARFILSHGRIGLSPDAGATWLLTQALPRPLVQ
                     QLVWLGEPVTAQQLHAWGVVASVVDQGHAVDAALRLAEGLAAMAPNALASGKELVAQA
                     AGCSLAQQLNAERDHFVANLFHANGAEGLNAFLEKRPPKFS"
     gene            3183..3887
                     /locus_tag="VNU48_02470"
     CDS             3183..3887
                     /locus_tag="VNU48_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWI10171.1"
                     /translation="MNSPVLTIVERSNIDAGSWFSRLSQPLRHAILSRATVRRLKDGA
                     MMSTRGASADEWCGVAKGAVRVSSVSLSGKQITLTYVEPGTWFGDISLFDGLPRTHDS
                     NAHGETTLLVVRKADFKELLAQHTELYEALLRLNCRRLRLLFDAVEDLNTLPLASRLA
                     KQILLLARSYGVAQGEEIRIGLQLAQEDLAQLLGASRQRVNQELKGFERDGAVRIEPT
                     RLVVLSKEKLLAISGK"
     gene            3921..>4331
                     /locus_tag="VNU48_02475"
     CDS             3921..>4331
                     /locus_tag="VNU48_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWI10172.1"
                     /translation="MEAQTGTKPVAQSHALDAAALQAYLEPRLEGFKGPLAVEQFKGG
                     QSNPTYKLVTPTRAYVMRSKPGPAAKLLASAHAVDREFMVMRALHGSDVPVPTMHVLC
                     EDEAVIGRAFYVMEFVEGRVLWDQALPAIATAQRA"
BASE COUNT          629 a         1514 c         1551 g          637 t
ORIGIN      
        1 ggctacgagc agcgcatcgc gcgcttctgc ttcgcgaaga aggacgagac cctgcagctg
       61 gcgctggcgc gcctggccag gctctgagcg ctgcgcggcg cggcacgctc agtggtaggt
      121 gccggacttg ggcggttccg gtggcgcgtc gaggttgaag tcgatcaggt gcggcgcgtc
      181 gagcttcggc cggtcggctt gacccagcgg cgaggtcttg gcgttctgcg ccgcggagtc
      241 cgagggcagg tcgaaatcca ggcgcgtcgt gcgcagcccg ccattcggca ggtcggccga
      301 cagcgaggtc gcactcagcc gcgaaatcgg ctcttcaccg gcgccatcga gtggcagcag
      361 caggtcgaca ccggcgctgg gcagcgcgcc gccgtgctcg gccaggtcgc gtgcgatcga
      421 gtagaggaac aacagctcgc ggtaggcggg caggtcgaag gtctcgtcgc tcttgttgcg
      481 ccggaacagc gacgcctcca gcgtctccat catccgcgcc ggtgacgacc acagcccctg
      541 cagccgcacg atcgtgtcgg gatagtcgac cagcgaacgg ccctgctgcg ggtccgactc
      601 ccagtcaggt gcataggcgt tgaagcggcg gttgaaacgc tcgcgcacgc gctggtaggc
      661 ctcgcgttcg ccgcggcggc ggtagatctc gagcagcttc aggtacggca acgggctgtt
      721 gccgccatcg ctgcgcacat ggctcatcaa caggtcgatc gcggcttcgt cctgaccgag
      781 gacgatgaag aactcgacct gctgttccag atcgatcagc tcctcgaccg acagttcgcg
      841 ggccggcccg gacgtgtgcg agccttcgtc ctcgaccacc gtgggaacgg taaagacgtc
      901 ggtcgcgggt gcggccgcag ccgcaagctc cggtgccggt ggctcctcga gttcggcctt
      961 cgccatcgcg gccgcagcac cggcggccag cggcgtctgc gacggcgcgg gctgcggcgc
     1021 tgggcccgcc caccactgcc ccgactggcg cgcgcgcgac tggcgccagc cgagccagac
     1081 caccgccagc gccagcagcg ccgacagcgc ggccagcgcg tagaccagcg ggttcgcgta
     1141 gcgcgccgac tcggcgtcct gcacgcgggc ctgcatcgcg atcagcgacg cctggttggc
     1201 ctgcgcactc ttggtcagtt tcgcaagccc ctcctcgagc acctggatgc gttggcgctc
     1261 cgccgccaac gggtcggcga ccggatcggg cttgacggcg gatgcggcag cgtcggcgac
     1321 cagcggcgca gccgctggcg cggcggcgga ggcgccacgc gccacgatcg gcgccgccgt
     1381 ttcgaggtgc agccgcggcc cggccgtcgg cgcgcgtgcc acggcgggtt ggcgcgagat
     1441 cggccgcggc ttcgcggcct gcggtgtgat ggcttcgcgc gcactggcaa ccgaccgtgc
     1501 gcgcgacgcc gccgcacgcg cgctgcggcg cgcggcgctg gcggccggca ccggccgcgc
     1561 ggtgatgatc gacgtctcgg cgcccggccc ggcggccgcc gccggcgcgg acgcggccac
     1621 ggccgcctcg acgcgcggcg gcgccggtga agcctcggcc gccgccgttt gcggggcgtt
     1681 gatcaatggc gggtcgacga acgcgacaaa gcggcgcgtg atcttcgcga cgcagcccag
     1741 cgtcacgctg acggtgacga ccggttcgtc gatgaacgtc ccggtggtga cccgcacacc
     1801 gcggttgttc gaatcgaccg ggccttccag gctgacccgc accagactcg gtgcgagccg
     1861 gttctccccc gactggacct cggcgaagac gcattcaggc gccagctgct cgtcggcgtc
     1921 gagccggatc gcggcgagga agtccagcgg ctgcccgagc tgggtcgcgt tgacgacccg
     1981 cccgaacccg accgcgtggg ccggcatggc agcgatccac agcgcagtgc cggtcacgag
     2041 aagggtcgaa actgcgcgca tgcgggtgga gtggtggtgg tcgctgtgga tggccgaggg
     2101 actctagcat gaagagtccg ggctccagag cgccgccgcg gcgcgataac ggcgccttgc
     2161 gtgacacaat tccggccgcg cgcaccgctg cccgccattc gtgcgcgaaa gccggcgtcc
     2221 gcacctgcac ccgccctcct ccgtctcggg aatcccgatg tctgccgaac tcctgaccga
     2281 gcgccgcggc gccgcgctgc tgctgaccct gagcgacccc gcgacacgca acgcgctgtc
     2341 gcacgccgtg atcgccgccg gcatcgaggc gctgaacatg gccgaggccg attccgagct
     2401 gcgctgcgtg ctgctgcgcg gcgccggcgg ccatttctgc gccggcggca acctgcaggg
     2461 cctgctcgag cgccgccgca gcgggccacc ggcgcaggtg cgcatgctcg agcacctgca
     2521 ccagttcatc gaggcgctgc gcgtgttccc gaagccggtg atcgcggcgg tcgagggcgc
     2581 ggcggccggc gccgggtttt cgcttgcgct ggcctgcgac ctgatcgttg cggcggagga
     2641 cgcgcgcttc atcctgtcgc acggccgcat cggcctgtcg cccgacgccg gtgcgacctg
     2701 gctgctgacg caggcgctgc cgcgcccgct ggtgcagcag ctcgtctggc tcggcgagcc
     2761 ggtgacggcg cagcagttgc acgcctgggg ggtggtcgcc tcggtggtcg atcagggtca
     2821 cgcggtcgac gccgcgttgc ggctggccga gggcctcgcg gcgatggcgc cgaacgcgct
     2881 ggccagcggc aaggaactgg tcgcccaggc cgccggatgc tcgctggcgc agcagctgaa
     2941 cgccgaacgc gaccacttcg tcgcgaacct gttccacgcc aacggcgccg aagggctgaa
     3001 tgccttcctc gagaagcggc cgccgaagtt cagctgagcg ctcgcgcgcg gacctggatc
     3061 gactgccgcg agatcggcag cacgccgccc ttctgcgccg acgaagggcc atccatgcgc
     3121 ctgacacacc atacacaata ggccggcgtg gcggcatgat gctgtcacat cagcgacgaa
     3181 acatgaatag ccccgttctc acaatcgtcg aacgctccaa catcgacgcc ggctcttggt
     3241 tctccaggct ctcccaaccc ctgcgccatg cgatcctgtc gcgcgcgacg gttcggcgcc
     3301 tgaaggacgg cgcgatgatg tccacgcggg gcgcgtcggc cgacgaatgg tgcggcgtgg
     3361 ccaagggcgc ggtgcgcgtc agttcggtgt cgttgtcggg caagcagatc acgctgacct
     3421 acgtcgagcc cggcacctgg ttcggcgaca tctcgctgtt cgacggcctg ccccgcacgc
     3481 acgactcgaa tgcgcatggc gagaccacgc tgctggtggt gcgcaaggcc gacttcaagg
     3541 aactgctggc gcagcacacc gagctctacg aggcgctgct gcgcctgaac tgccggcgcc
     3601 tgcgcctgct gttcgatgcg gtcgaggatc tgaacacgct gccgctcgcg tcgcgcctgg
     3661 ccaagcagat cctgctgctg gcgcgcagct acggtgtggc ccagggcgag gagatccgca
     3721 tcgggctgca gctcgcccag gaagacctgg cgcagttgct cggcgcctcg cgccagcgcg
     3781 tcaaccagga gctcaagggc ttcgaacgcg acggcgcggt gcgcatcgag ccgacgcggc
     3841 tggtggtgct gtcgaaagag aagttgctcg cgatttccgg caagtaacga cgatgatgaa
     3901 ttcactccga ggtcgatgac atggaagccc agaccggcac caagcccgtt gcacagtcgc
     3961 atgcactcga cgcggccgcc ttgcaggcct acctcgagcc gcgtctcgaa ggtttcaaag
     4021 gcccgctcgc ggtcgagcag ttcaaggggg gccagtcgaa cccgacctac aagctggtca
     4081 cgccgacgcg cgcctacgtg atgcgctcca agcccggccc cgcggcgaag ctgctcgcgt
     4141 cggcgcatgc ggtggaccgc gagttcatgg tgatgcgcgc gctgcacggc agcgacgtgc
     4201 cggtgccgac catgcacgtg ctgtgtgaag acgaggccgt gatcggccgg gccttctacg
     4261 tgatggagtt cgtcgagggc cgggtactgt gggatcaggc gctgccggcg attgccaccg
     4321 cccagcgcgc c
//
LOCUS       DATMJB010000111         4207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_399600, whole genome shotgun sequence.
ACCESSION   DATMJB010000111 DATMJB010000000
VERSION     DATMJB010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399600"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VNU48_02480"
     CDS             <1..1045
                     /locus_tag="VNU48_02480"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWI10173.1"
                     /translation="PTTPSVADRMMPVDQYIGGVEHAILHLLYSRFFTRAMKATGHIG
                     MDEPFAGMFTQGMVVHETYSKADGTYVTPAEVKIETGGDGRRASLLTTGEDISIGAIE
                     KMSKSKRNTVDPDDIIATYGADVARWFMLSDSPPDRDVIWSDERVQGASRFVQRLWRL
                     VGESADFAKAAPAARPASFGPDAQAIRKAAHGALDKVSTGIEKLHFNVCLAYIREFSN
                     ALSEALAREGQSAPDLTWSVREAAVILVQLFAPMMPHLAEECWTVLGQSGLVSEANWP
                     KIERDLLVEDTVTLVVQVNGKKRGEVTVGRDAQNPQIEAAVLGLDAVKQALDGKAVRK
                     VIIVPMRIVNVVG"
     gene            1032..1583
                     /gene="lptE"
                     /locus_tag="VNU48_02485"
     CDS             1032..1583
                     /gene="lptE"
                     /locus_tag="VNU48_02485"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HWI10174.1"
                     /translation="MSLARTRIAARLLAVAALAALTAGCFQPMYAERADGAPGLREKL
                     MGVEVPPVDKANASREARIQVEIRNALAFKLYGNATGMPPTHRLVLRFTTSRSSLMLD
                     PATALPSSENYGIDAQYNLIELASNKSVMTGSTFSRVSYDVPGQLQRFARARAFRDAE
                     DRAAQEIAENIQTRLASFFVAGT"
     gene            1591..2619
                     /gene="holA"
                     /locus_tag="VNU48_02490"
     CDS             1591..2619
                     /gene="holA"
                     /locus_tag="VNU48_02490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWI10175.1"
                     /translation="MVALRGKEIDAFLARPDPGRPIILLYGPDAGLVRERADALLASA
                     VDDPNDPFSLVRLDGDELSAEPSRLVDEAMTVPLFGGRRAIRVRAGSRSFAGGIDTLA
                     EMPLKDCRIVIEAGELRPESPLRKACERARTAVAIGCYPDGERDLAKLIDDELKVSNL
                     RIAPDARAALTMLLGGDRQASRNELKKLALYAHGQTEVTLDDVMAVVADASELKIDPI
                     VDGAFAGNSAAVETEFAKAMIAGIYPGMIISAAQRQAAWLHKTSLAVAEGTPISTLLD
                     SGFPRLHFSRKGNVETALRHFNAARLLTIIEQLATAALETRKQASLAAAIAQRALLSI
                     AVNAKRRG"
     gene            complement(2623..3501)
                     /locus_tag="VNU48_02495"
     CDS             complement(2623..3501)
                     /locus_tag="VNU48_02495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWI10176.1"
                     /translation="MADEARSRLGRGLASLIGDVGGEAAHVDRPRAQRKVPIEFLKAN
                     PRNPRRAFSDAELGELSDSIKQHGVIQPIVVRPVKGVQDRYEIIAGERRWRASQIAGL
                     HEVPIVPVDVSDSAALEIAIIENVQREDLNAMEEAQGYHALADEFKRSQEEIAKIVGK
                     SRSHVANTMRLTKLPAEVQGYIASGKLSAGHARALIGVPDPLAAAKRIVEEGLNVRQA
                     EALAHIEGVPERKPQKPRGGKVKDPDTVALEKRVTDALGLKVSVDHRDPGGVVQIRYG
                     DLEQLDEVIRRLAKGG"
     gene            complement(3608..>4207)
                     /locus_tag="VNU48_02500"
     CDS             complement(3608..>4207)
                     /locus_tag="VNU48_02500"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWI10177.1"
                     /translation="EAPLRESVVPTAVPRLHIATSTMDLSGLELELGTTPGRAFRLRD
                     AIAALNNDAAPGSDYTYVLIDCPPSLNLLTVNAMAASDAILVPLQCEFFALEGLSQLL
                     QTVEQVRSTLNPNLSIHGIVLTMFDSRNNLSNQVVADVRQFMGSKVYNTMIPRNVRIS
                     EAPSHGKPVLVYDLKCVGSEAYLKLATEVIQRERELRAH"
BASE COUNT          724 a         1347 c         1367 g          769 t
ORIGIN      
        1 gccgaccacg ccatccgtgg cggatcggat gatgccggtc gatcagtata tcggcggcgt
       61 cgagcacgcg atcctgcatc tgctgtacag ccgtttcttc acccgcgcga tgaaggccac
      121 cggccacatc ggcatggatg agccgttcgc cggtatgttc acgcaaggca tggtggtgca
      181 cgagacctac agcaaagccg acggcactta cgtcacgccg gccgaagtga agatcgagac
      241 tggcggcgat ggccgccgcg ccagcctgct aaccacgggc gaagacatca gcatcggcgc
      301 gatcgagaag atgtcgaagt ccaagcgcaa cacggtcgat cctgacgaca tcatcgcgac
      361 ctacggcgcc gacgtcgcgc gctggttcat gctgtcggat tcgccgccgg accgcgacgt
      421 gatctggagc gacgagcggg tgcagggcgc atcgcgcttc gtgcagcggc tgtggcggct
      481 ggtgggcgaa tcggccgact ttgccaaggc ggccccggcc gcgcggccgg cttcgttcgg
      541 ccccgacgcg caggccatcc gcaaggcggc gcacggcgcg ctcgacaagg tctcgaccgg
      601 catcgaaaaa ctgcatttca acgtctgcct tgcctatatc cgggaatttt cgaatgcgct
      661 gagcgaggcg ctggcgcgcg aggggcagtc ggcgcccgac ctgacctggt cggttcgcga
      721 ggccgcggtc atccttgttc aactgttcgc gcccatgatg cctcatctgg ccgaggagtg
      781 ctggacggtg ctggggcagt cggggctggt ttccgaggcc aattggccca aaatcgaacg
      841 agatttgctg gttgaagaca cggtgaccct ggtggttcag gtcaatggca agaagcgggg
      901 cgaggtcacg gtgggccggg atgctcaaaa tccgcaaatt gaggctgccg ttttgggtct
      961 cgatgcggta aaacaggcct tggacggcaa ggccgtccgc aaggtgatta ttgttcccat
     1021 gaggatcgtg aatgtcgttg gctaggaccc gcatcgccgc tcggcttttg gccgtggccg
     1081 cattggcggc gctgacggcc ggttgctttc agccgatgta tgccgagcgc gccgacggcg
     1141 cgccggggct gcgcgaaaag ctgatgggcg ttgaagtccc gccggtggac aaggccaatg
     1201 cttcgcgcga ggcccgtatc caggtggaga tccgcaatgc gctggcgttc aagctgtatg
     1261 gcaacgccac cggcatgccg ccgacccatc ggctggtgct gcgattcacc accagccgct
     1321 cgtcgctgat gctggatccc gccaccgcgc tgccctcgag cgaaaactat ggcatcgacg
     1381 cccagtacaa tctgatcgag cttgcctcga acaagtcggt gatgaccggc agcacgttct
     1441 cccgcgtgtc ctatgacgtg ccgggccagt tgcagcgctt cgcccgcgcc cgcgccttcc
     1501 gcgacgccga agaccgcgcc gcccaggaga tcgcggaaaa tatccagacc cggctggcgt
     1561 cgttcttcgt cgccggcacc tgaccgacca ttggtcgcgc tccgcggaaa agagatcgac
     1621 gcttttctcg cccggcccga tcccggtcgt cccatcatcc tgctttacgg ccccgatgcc
     1681 ggcctcgtcc gcgagcgcgc cgacgcgttg ctggcctccg cggtcgatga tcccaacgat
     1741 ccgttttcat tggtgcggct ggatggcgac gaattgtccg ccgagccgtc gcggctggtc
     1801 gatgaagcca tgacggtgcc gctgttcggc ggccgccgcg ccatacgcgt tcgcgccggc
     1861 tcgcgcagtt ttgccggcgg catcgacacc ctggccgaga tgccgctgaa ggattgccgc
     1921 atcgtgatcg aggccggcga actgcgtccg gaatcgccgc tgcgcaaggc ctgcgagcgc
     1981 gccaggaccg cggtcgcgat cggctgctat ccggacggcg aacgcgacct tgcgaaactg
     2041 atcgacgacg aattgaaggt gtcaaattta cgcatcgcgc cggacgcgcg cgccgcattg
     2101 acaatgctgc tcggcggcga tcgccaggcc tcgcgtaacg aactcaagaa gcttgcgctg
     2161 tatgcccatg gccaaaccga ggtcacgctc gatgacgtga tggcggtggt cgcggatgct
     2221 tccgagctca agatcgatcc gatcgtggac ggcgcctttg ccggaaattc cgcagcggta
     2281 gagacagaat tcgccaaggc catgatcgcc ggtatctatc caggcatgat tatatccgcg
     2341 gcgcagcgcc aggcggcgtg gctgcacaag accagcctcg cggtggccga gggcacgccg
     2401 atatcaactt tgctcgacag cggatttccg cggctgcact tttcccgaaa gggaaacgtc
     2461 gaaaccgcgc tacgccattt caacgcggcg cggcttctga caatcatcga gcaactggcg
     2521 accgcggcgc tggaaacgcg caagcaggcc tcgctggcgg ccgcgatcgc ccagcgcgcg
     2581 ttgctgtcga ttgcggtgaa tgcgaagcgg cgggggtgac gactagccgc ccttcgccag
     2641 tctccgtatc acctcgtcga gctgttcgag atcgccgtag cggatctgca ccacaccgcc
     2701 gggatcgcgg tgatcgacgc taaccttcag accaagcgcg tcggtgacgc gcttctccag
     2761 cgccaccgtg tcgggatctt ttaccttgcc gccgcgaggc ttctgcggtt tgcgctccgg
     2821 cacgccctcg atatgcgcca gcgcttcggc ctgacgcaca ttgaggcctt cctcgacgat
     2881 gcgtttcgcg gccgccagag gatcgggcac accgatcagg gcgcgcgcgt gacccgccga
     2941 cagtttcccc gacgcgatgt agccctgcac ttcggccggc aatttcgtca gccgcatcgt
     3001 gttggcgaca tggctgcggc tcttgccgac gattttcgcg atctcttcct ggctgcgttt
     3061 gaattcatcg gcgagggcgt gatagccctg cgcctcctcc atcgcattga ggtcctcgcg
     3121 ctgcacattc tcgatgatcg cgatctcgag cgcggcactg tcgctgacgt cgaccggaac
     3181 gatcggcact tcgtgcaggc ccgcgatttg cgacgcgcgc cagcggcgct cgccggcgat
     3241 gatctcgtaa cggtcctgca cgccctttac cggacgcacc acgatcggct ggatcacgcc
     3301 gtgctgcttg atcgagtccg agagctcgcc gagctcggca tcggaaaagg cccggcgcgg
     3361 gttgcgcgga ttggccttga ggaattcaat cggcaccttg cgctgcgcgc gcggccgatc
     3421 gacatgcgcg gcctcgccgc cgacatctcc gatcagactt gcaagaccgc ggcccaatcg
     3481 cgaacgcgct tcgtcggcca tcgccagctc ccttggattc actcgcatcg ctccggaatt
     3541 agacgtttcg gttttcaggt ttgccgagga cgggtcgagc gaagcgaacc catcctgcaa
     3601 ttcagcttca atgtgcgcgc agctcgcgct cgcgctggat cacttcggtg gcgagcttga
     3661 ggtaggcttc gctgccgacg catttgagat cgtacaccag caccggcttg ccgtgagacg
     3721 gcgcttcgga gatgcgcacg ttgcgcggga tcatggtgtt gtagaccttg ctgcccatga
     3781 actgccggac gtcggcgaca acctgattcg ataaattgtt gcgcgagtcg aacatggtca
     3841 gcacgatgcc gtggatcgac aaattgggat taagcgtcga acgcacctgc tccaccgtct
     3901 gcagcaattg cgacaggcct tcgagcgcga agaactcgca ctgcagcggc accaggatgg
     3961 catcggaagc cgccatcgcg ttgacggtga gaagattgag cgacggcggg cagtcgatca
     4021 gcacataggt gtagtcggat ccgggcgccg catcgttgtt cagtgccgcg atggcgtcgc
     4081 gcagccggaa ggcgcggccg ggcgtggtgc cgagctcgag ctcgaggccg gagagatcca
     4141 tggtggaggt cgcgatgtgc agccgcggca ccgcggtggg caccaccgat tcgcgcaagg
     4201 gcgcttc
//
LOCUS       DATMJB010000112         2900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_458577, whole genome shotgun sequence.
ACCESSION   DATMJB010000112 DATMJB010000000
VERSION     DATMJB010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458577"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..748)
                     /locus_tag="VNU48_02505"
     CDS             complement(143..748)
                     /locus_tag="VNU48_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10178.1"
                     /translation="MPVERNTLDAARLNPSAVLPFELRLEDFALAIQDVYDFFFDVNV
                     SLVAKGLERLDDTLRPAIMSGVLSDMLTASLAKHSRALVVNSYFNGHPDLVVKGRYAN
                     NAAKAGEHGVEIKTTRKAGGAVDTHGARDQWMCVFVYEVDNVGEPATARSPMTFTEVY
                     LGQVGIADFRKNARGELGTRTATLHAEGIAKLRSSWIYLAR"
     gene            complement(797..2695)
                     /gene="recQ"
                     /locus_tag="VNU48_02510"
     CDS             complement(797..2695)
                     /gene="recQ"
                     /locus_tag="VNU48_02510"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HWI10179.1"
                     /translation="MTPLAAATSPDTILHDVFGYSAFRGAQAEIVEHVIDGGDALVLM
                     PTGGGKSLCYQVPAIARHRAERGVAVVVSPLIALMHDQVGALEEAGVHASFLNSTLTL
                     DEVQRIEREMMSGRLVLLYVAPERLTTPRMLAQLDSLDERGLLSLFAIDEAHCVSQWG
                     HDFREDYLALNVLHERYPGVPRIALTATADELTRADIIKRLQLEDARVFISSFDRPNI
                     RYAVVEKVEPRQQLLRFLQDEHEGEAGIVYCQSRKKVDETAAWLSSEGITALAYHAGL
                     DSDVRRRHQDRFLREDAVVMVATIAFGMGIDKPDVRFVAHLDLPKNIEAYYQETGRAG
                     RDALPADAWMAYGLADVVNQRRMIDESPAAEEFKRGQRGKLDALLSLAEAHDCRRVRL
                     LAYFGEQSAPCAAYQNACDNCLNPPATWDATEAARKALSCIYRFHQHRGQRFGAGHLI
                     DVLRGKATDKVAQYGHESLSTFGIGADVSEAQWRVVLRQLIALGHLRTEGEYNTLELT
                     ATAREVLRGDVRMLLRQASDAPKRSGRSGKTAKARGGKDKPPRAPGALLPLDAEGLAR
                     FEALKAWRAEVAREHNLPAYIVFHDATLAEMAQAAPDSIDALGEISGVGAKKLQAYGS
                     EILRVLAR"
BASE COUNT          417 a          983 c         1022 g          478 t
ORIGIN      
        1 gtgttgtgcg ccggtcatcg gcctgcacgg tggagcagat cgggccgaaa gcagagatcc
       61 aaagccgcag cagcgtttca ttcgtcgtgg gtcgcgcaat gcgtggtggg gcaactgatc
      121 tgaaggccgg cgccggcagc cgctacctgg ccaggtagat ccagctgctg cgcagtttgg
      181 cgatcccttc ggcgtggagc gtggccgtgc gggttcccag ctcgccgcgg gcgttcttgc
      241 ggaagtccgc gatgccgacc tggccgagat agacctcggt gaatgtcatc ggcgaacgcg
      301 ccgtggcggg ctcgccgacg ttgtcgactt cgtagacgaa gacgcacatc cattgatcgc
      361 gggcaccgtg cgtgtcgacg gcgccgccgg ccttgcgggt ggtcttgatc tcgacgccgt
      421 gctcgccggc cttggccgcg ttgttcgcgt atcgaccttt gacgaccaga tccggatggc
      481 cgttgaagta gctgttcacg accagcgcgc gcgagtgttt cgccaggctc gcggtgagca
      541 tgtctgacag cacgcccgac atgatcgccg gtcgcagcgt gtcgtcgagg cgctccaggc
      601 ccttcgcgac aaggctcaca ttcacgtcga agaaaaagtc gtagacgtcc tggatcgcca
      661 gcgcgaagtc ttcgagccgc agttcgaacg gcaagaccgc gctcgggttc agccgggcgg
      721 catccagcgt gtttctctcg acaggcatcc ggggtttccg actactcgag tggcgtggcg
      781 accaacaccg cgatggtcat cgggccagca cccgcaggat ctcggatccg tacgcctgca
      841 gcttcttcgc ccccacgccg ctgatttcgc cgagcgcgtc gatcgaatcg ggtgcggcct
      901 gcgccatctc ggccagcgtc gcatcgtgga acacgatgta ggccggcagg ttgtgctcgc
      961 gcgcgacctc ggcgcgccag gccttcagcg cctcgaagcg cgccaggccc tcggcgtcca
     1021 gcggcaacag cgcccctggg gcgcgtggcg gcttgtcctt gccgccgcgc gccttcgcgg
     1081 tcttgccgct gcggccgctg cgcttgggcg cgtcgctggc ctggcgcagc agcatgcgca
     1141 cgtcgccgcg cagcacctcg cgcgccgtcg cggtcagctc cagcgtgttg tattcgccct
     1201 cggtgcgaag atggccgagc gcgatcagct ggcgcagcac gacgcgccac tgcgcctcgc
     1261 tcacgtcggc gccgatgccg aaggtcgaca ggctctcatg cccgtattgc gcgaccttgt
     1321 cggtggcctt gccgcgcagc acatcgatca ggtgaccggc accgaaacgc tggccgcggt
     1381 gctggtgaaa gcggtagatg cacgacagcg ccttgcgcgc ggcctcggtc gcgtcccagg
     1441 tggcgggcgg gttcaggcag ttgtcgcagg cgttctggta cgcggcgcag ggtgcgctct
     1501 gttcgccgaa ataggcgagc aggcgcacgc gccggcaatc gtgcgcctcg gccagcgaca
     1561 gcagcgcgtc gagcttgccg cgctggccgc gcttgaattc ctcggccgcc gggctctcgt
     1621 cgatcatgcg gcgctggttc acgacatcgg ccaggccgta ggccatccag gcgtcggccg
     1681 gcagagcgtc gcggccggcg cggccggtct cctggtagta ggcctcgatg ttcttcggca
     1741 ggtccaggtg cgcgacgaag cgcacgtcgg gcttgtcgat gcccatgccg aacgcgatcg
     1801 tcgcgaccat cacgacggcg tcctcgcgca ggaagcggtc ctggtggcgg cgccgcacgt
     1861 cgctgtcgag gccggcgtgg taggccagtg cggtgatgcc ctcgctgctc agccacgcgg
     1921 cggtctcgtc gaccttcttg cgcgactggc agtagacgat gccggcctcg ccctcatgct
     1981 cgtcctgcag gaaacgcagc agctgctggc gcggctcgac cttctcgacc acggcgtagc
     2041 ggatgttcgg ccggtcgaag ctgctgatga agacgcgcgc gtcttcgagc tgcagccgtt
     2101 tgatgatgtc ggcgcgggtc agctcgtcgg cggtggcggt cagcgcgatg cgcggcacgc
     2161 cggggtagcg ctcgtgcagc acgttgagcg cgagatagtc ctcgcggaag tcgtggcccc
     2221 actggctgac gcagtgcgcc tcgtcgatcg cgaacaggct cagcaggccg cgctcgtcca
     2281 gcgagtcgag ctgcgccagc atgcgcggcg tcgtcaggcg ttcgggcgcg acgtacagca
     2341 gcaccaggcg cccactcatc atctcgcgct cgatgcgctg cacctcgtcg agcgtcagcg
     2401 tcgaattgag gaacgacgcg tgcacgccgg cctcctcgag cgcgccgacc tggtcgtgca
     2461 tcagcgcgat cagcgggctc acgacgaccg ccacgccgcg ctcggcgcgg tgccgcgcga
     2521 tcgccggcac ctgatagcac aggctcttgc cgccgccggt gggcatcagc acgagcgcgt
     2581 cgccgccgtc gatcacgtgc tcgacgatct cggcctgcgc gccgcggaag gcgctgtagc
     2641 cgaacacgtc gtgcaggatc gtgtcggggg aggtggcggc ggcgagaggg gtcacggggc
     2701 gggcggggcg gcaaagccgc gatggtacgc ggggcaccgc gtcgacccac tcgcccgttc
     2761 gggggttcga cgggggaacc cccccgttcc cgccaaccgg gggaaggtgc agcgagggcg
     2821 gcgagcgata attcacccaa tcctcgaatt gccccaccag ccccccggag cgcacccatg
     2881 gcctcagtca acaagatcgg
//
LOCUS       DATMJB010000113         4572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_108061, whole genome shotgun sequence.
ACCESSION   DATMJB010000113 DATMJB010000000
VERSION     DATMJB010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108061"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VNU48_02515"
     CDS             <1..402
                     /locus_tag="VNU48_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HWI10180.1"
                     /translation="TAHATSTGGREGTSRSSDGVLDLKLTTPKEMGGSGATGTNPEQL
                     FAAGYSACFIGAMKYVAMTQKITLPADTSIKADVGIGPIPAGFGIQVALNVTIPGMGR
                     AAAEKLVAAAHQVCPYSNATRGNIEVTLTVV"
     gene            complement(454..1794)
                     /locus_tag="VNU48_02520"
     CDS             complement(454..1794)
                     /locus_tag="VNU48_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HWI10181.1"
                     /translation="MSVAMTTAMGPQAPLAHQLAEARRLARRAAMVLIVGVVPVAAWL
                     TLAQLSAAVVASSFVKVDLDRRVVQHAEGGTVREVKVRDGQHVEKGEALLVLGDVSVD
                     ADLNRLDYRVAVERASLARLEAEQLSAAAVHFPADVVEAARTDPRVAEQLAKERMLFS
                     ARRDALVGQSVLLRSQQAKVVQEATALRAQIAQATESLRHQKDELEVNRRLLNDGFIS
                     ATRISQLEATVADYGVKLEEKRTDFARAEQRLLDADLRIKSLEGDYRQQASDQLKVSA
                     AKLAELQQEQRKTVDASSRQVITAPVAGDVINLKFTTPGSVVSPREPIADIVPTNPRL
                     VVEAHIRTEDVSRVQQGQPADIRFTAFKYRTTRMVSGKVFYLAADRTLDHATNQAYYV
                     ALVEADAASLANAGEVKLQAGMPAEVYIKGEERTPLQYLVEPVTQVLRRAGRER"
     gene            complement(1791..3545)
                     /locus_tag="VNU48_02525"
     CDS             complement(1791..3545)
                     /locus_tag="VNU48_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HWI10182.1"
                     /translation="MKPLIELLKRPLLHVAGLSFFVNLLLLVPALFMLQVFDRVLASQ
                     SGETLVVLMIGVGVGLALLFALDYLRARLQGVAGNIVAESLSPVVTKIVIEQGARRSG
                     RSSSESLRDVGSLRALFSAQGLLALIDSPWVFAYVAVIWFAHPLLGIAAACAAVLMLA
                     LAVINDVVTRREIEALQKAAAGATRYLEASLQNAEIAQTLGMTDALVARWRAKNAEVT
                     ALQQPTATKTVAMAALTRTVRQAVQVLMLALGAWLVISGEASAGVMIATTTLLGRALA
                     PVEQVVGSWRILAEGRAAFRRLSELLNAAATEPQRMALPAPSGELVAQNLVFRAPQGE
                     RMLLAGVSLQLEAGESLAVIGASGAGKSTLLRLLTGVWKPSAGTVRLDNADLAQWPRD
                     ELGPWLGYVPQDVELFPGTVAENIARLGEVDSARVVRAAQRAQVHELILALPNGYDTV
                     VDPQGALISPGQRQRIALARALYGEPKLVILDEPNSNLDGAGEAALGEALKALRGEAT
                     VVVVTHRATLIQHVDKVLVLDAGKVQHYGPAAEVMRAMQQKAAPAGGGGQVVTMPARA
                     AHAHEHGVAPAAREEKVS"
     gene            complement(3604..>4572)
                     /locus_tag="VNU48_02530"
     CDS             complement(3604..>4572)
                     /locus_tag="VNU48_02530"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HWI10183.1"
                     /translation="AGVTLDYEAKTSYAVNVNVNDPSVGGNPDATQSFALNVNNLAEG
                     VAATDLQLVVDAIPNANGLPTGAFAHLAIVDTDGGGAHSYSMTTTVNGVLSSALTVDG
                     STGVVSTTGLAAGTVYALDVQATQAGAASYSETLYLTTGTNADNNPLNGSGDDVIYAL
                     NGTDIVLAGSGNDTVFGQGGNDTLNGGDGDDLLYGGAGTNQLTGGAGSDTFVFDASGV
                     SSALDFHATPTATEVDMVMLSGGFNNIANDATGHLSSADFVSLASGGDTVAVGAAQNI
                     IFDRNTGNLYYDSNGGSSASRSLIGHIDVADGTPATFDFNDIKVGP"
BASE COUNT          706 a         1601 c         1523 g          742 t
ORIGIN      
        1 accgcccacg ccacctcgac cggcggccgc gagggcacgt ccaggtcgtc cgacggcgtg
       61 ctcgacctga agctgaccac acccaaggaa atgggcggca gcggcgccac cggcacgaac
      121 cccgagcagc tgttcgcggc gggttactcg gcctgcttca tcggcgcgat gaagtacgtc
      181 gcgatgacgc agaagatcac gctgccggcc gacacctcga tcaaggcgga cgtgggcatc
      241 ggcccgatcc cggccggctt cggcatccag gttgcgctga acgtgacgat cccgggcatg
      301 gggcgcgccg ctgccgagaa gctggtcgcg gccgcgcatc aggtgtgccc gtactcgaac
      361 gcgacgcgcg gcaacatcga ggtgacgctg accgtggtct gagcgcgatc ggctcggcac
      421 ccgcccgggc ggggccgcgg ccatcgagtc ggctcagcgt tcgcggcctg cgcgccgcag
      481 cacctgcgtc accggctcga ccaggtactg cagcggcgtg cgctcctcgc ccttgatgta
      541 gacctcggcc ggcatgccgg cctgcagctt cacctcgccg gcattggcga gcgaggcggc
      601 atcggcctcg accagggcca cgtagtaggc ctggttggtc gcgtgatcga gcgtgcggtc
      661 ggccgccagg tagaacacct tgccggacac catacgcgtc gtgcggtact tgaaggcggt
      721 gaagcggatg tccgccggct gaccctgctg cacccggctc acgtcctcgg tgcgaatgtg
      781 cgcctcgacc accagccgcg gattcgtcgg cacgatgtcg gcgatcggct cgcgcggcga
      841 gaccacgctg cccggcgtcg tgaacttcag gttgatcacg tcgccggcca ccggcgcggt
      901 gatcacctgc cggctcgacg catcgacggt cttgcgctgc tcctgctgca gctcggcgag
      961 cttggccgcg ctgaccttca gctgatcgct ggcctgctgt cggtagtcgc cctcgagcga
     1021 cttgatgcgc aggtcggcat cgagcaggcg ctgctcggcg cgggcgaagt cggtgcgctt
     1081 ctcctcgagc ttgacgccgt agtcggcgac cgtggcttcc agctgcgaga tgcgtgtcgc
     1141 ggagatgaag ccgtcgttca gcaatcggcg gttgacctcg agctcgtcct tctggtgccg
     1201 cagcgactcg gtggcctggg caatctgggc gcgcaacgca gtcgcttcct gcaccacctt
     1261 ggcctgttgc gagcgcagca gcaccgactg gccgacgagc gcgtcgcggc gcgcgctgaa
     1321 cagcatccgc tccttggcca gctgctcggc cacgcgcggg tcggtgcgag ccgcctcgac
     1381 gacgtcggcg ggaaaatgca cggccgcggc cgacaactgc tcggcctcga ggcgcgccag
     1441 gctcgcgcgc tcgacggcca cgcggtagtc gagccggttc aggtccgcgt cgaccgagac
     1501 gtcgccgagc accagcagcg cctcgccctt ctcgacatgc tggccgtcac gcaccttgac
     1561 ctcgcgcacc gtgccgccct cggcgtgctg gacgacccgg cggtccaggt cgaccttgac
     1621 gaacgaactc gcgaccaccg ccgcggagag ctgcgccaac gtcagccatg cggccaccgg
     1681 gaccacgccg acgatgagca ccatggcggc acggcgcgcc agccggcggg cctcggccag
     1741 ttgatgcgcc agcggcgcct gcggacccat cgccgtcgtc atcgcgacgc tcacgagacc
     1801 ttctcctcgc gggccgccgg ggccacgcca tgttcatgcg catgcgcggc gcgcgccggc
     1861 atcgtgacga cctggccacc acctcccgcg ggcgctgcct tctgctgcat cgcgcgcatc
     1921 acctcggcgg ccggcccgta gtgctggact ttgccggcat cgagcacgag caccttgtcg
     1981 acatgttgga tcagcgtcgc ccgatgcgtc acgacgacca cggtcgcctc gccgcgcagt
     2041 gccttcagcg cttcgccgag cgccgcttcg ccggcgccgt cgaggttcga gttcggctcg
     2101 tcgaggatca ccagtttcgg ctcgccgtag agcgcgcggg ccagcgcgat gcgctgacgt
     2161 tgtcccggcg agatcagcgc accctgcggg tcgacgaccg tgtcgtagcc gttcggcagc
     2221 gcgaggatca gttcgtgaac ctgggcgcgc tgggcggcac gcacgacccg cgctgaatcg
     2281 acctcgccca ggcgcgcgat gttctcggcc acagtgcccg gaaacagttc gacgtcctgc
     2341 ggcacgtagc ccagccacgg gccgagttcg tcgcgcggcc actgcgcgag gtcggcgttg
     2401 tcgagccgca cggtgccggc gctcggcttc cacacacccg tgagcaggcg caacagtgtc
     2461 gacttgccgg cgccgctcgc gccgatcacc gcgagcgact cgccggcctc gagctgcagc
     2521 gacacaccgg caagcaacat gcgctcgccc tgcggtgcgc ggaacaccag gttctgtgcg
     2581 accagctcgc cgctcggcgc gggcagcgcc atgcgctgcg gctcggtggc ggccgcgttc
     2641 agcaactcgc tcagacggcg gaatgccgcg cggccttcgg caaggatgcg ccagctgccg
     2701 accacctgct ccaccggcgc aagcgcgcga ccgagcaggg tcgtggtcgc gatcatcaca
     2761 cccgcactgg cctcgccgct gatcacgagc caggcgccga gcgccagcat cagcacctga
     2821 accgcctgac gcacggtgcg cgtcagcgct gccatcgcga cggtcttggt ggcggtgggc
     2881 tgctgcagcg ccgtgacctc ggcattcttc gcccgccagc gcgcgaccag cgcatcggtc
     2941 atgcccagcg tctgcgcgat ctcggcgttc tgcagcgagg cttcgaggta gcgtgtcgca
     3001 cccgccgcgg ccttctgcag cgcttcgatc tcgcgccgag tgacgacatc gttgatcacg
     3061 gccagcgcga gcatcaacac ggccgcgcat gccgcggcga ttccgagcag cggatgggcg
     3121 aaccagatca ccgcgacgta cgcgaagacc cagggcgagt cgatcagcgc gagcaggcct
     3181 tgcgcagaga acagcgcgcg cagcgagccg acgtcgcgca ggctttccga actgctgcgg
     3241 ccgctgcggc gcgcgccctg ttcgatgacg atcttggtca ccaccggcga cagcgactcc
     3301 gcgacgatgt tgccggccac gccttgcaag cgcgcccgca gatagtccaa cgcgaacagc
     3361 agcgcaagtc cgacccccac accgatcatc agcacgacca aggtctcgcc gctctggctg
     3421 gccagcacgc ggtcgaagac ctgcagcatg aacagcgccg ggacgagcag cagcaggttg
     3481 acgaagaagg acaggccggc gacgtgaagg agcgggcgct tgagaagttc gatgagtggt
     3541 ttcatggtcc aacggtattt cggctgctac cacccggcct tgagggccgg gcagtaggtg
     3601 aaatcacgga ccgaccttga tgtcgttgaa gtcgaacgtc gctggcgtgc catctgccac
     3661 atcgatgtgc ccgatcagcg aacggcttgc gctactgcct ccgttgctgt cgtagtagag
     3721 gttgccggtg tttcgatcga agatgatgtt ctgggccgct cccacggcga ccgtgtcacc
     3781 gccactggcg agggatacga agtccgccga actgagatgc cccgtagcgt cgttggcaat
     3841 gttgttgaat ccaccggaca gcatgaccat gtcgacttcg gtcgcggtcg gcgtggcatg
     3901 gaaatccaag gcactcgaga caccggacgc atcgaacacg aacgtgtccg agccggcccc
     3961 gccggtgagt tggttcgtcc cggctcctcc atacaggagg tcgtcgccgt cgccgccgtt
     4021 caaggtgtcg ttgcccccct gcccgaagac cgtgtcgttg ccgctgcctg ccagcacgat
     4081 atccgtgccg ttcagggcat agatgacgtc atcgcccgag ccgttcaacg gattgttgtc
     4141 ggcgttcgtg cccgttgtga ggtacagcgt ttcgctgtat gacgccgcgc ctgcctgtgt
     4201 ggcctgaacg tcgagcgcgt agaccgtgcc tgcggccaat ccagtcgtcg ataccacccc
     4261 cgtcgatccg tccacggtca gcgcgctgga cagcactccg ttcaccgtgg tcgtcatgct
     4321 ataggagtgg gcaccgccgc cgtcggtgtc gacgatcgcc agatgcgcga aggcgccggt
     4381 gggcaatcca ttggcgttgg gaatcgcatc gaccaccagt tgtagatcgg tagccgccac
     4441 gccctcggcc agattgttga cgttcagcgc gaagctttgg gttgcgtctg gattgccccc
     4501 gacactcggg tcattgacgt tgacgttgac cgcatagctc gtctttgctt cgtagtcgag
     4561 cgtgacgcct gc
//
LOCUS       DATMJB010000114         6894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_43347, whole genome shotgun sequence.
ACCESSION   DATMJB010000114 DATMJB010000000
VERSION     DATMJB010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6894
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43347"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VNU48_02535"
     CDS             <1..479
                     /locus_tag="VNU48_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005020082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWI10184.1"
                     /translation="SLGGIEGDIRRINVRATEIQMGDRSTVIVPNSEFITKVVRNVTL
                     ADPLGLVQLKLPVPLGSDAQQVRELLLQAFAADAEVLATPAPSVLLDGVEHGALLFNA
                     TGFVGSPRSAARIRSAVLFDVLKRLADAGIALSSPPTMLISAAAPVAPQANPAAPA"
     gene            complement(480..836)
                     /locus_tag="VNU48_02540"
     CDS             complement(480..836)
                     /locus_tag="VNU48_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10185.1"
                     /translation="MVLGFAVSMVVLAALNIAGLDGLAPMIVVLLVGFGFLGLVVPTT
                     SVMALADHGEIAGAAAALMGMLQLVLGAVVIAVMGVFVDGTAWPMVAGIAVVAVVAWP
                     LTRWTLGSRPRVTAPA"
     gene            850..1017
                     /locus_tag="VNU48_02545"
     CDS             850..1017
                     /locus_tag="VNU48_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10186.1"
                     /translation="MTFSSAPQFRQCSKIQLEHPLEQPSPTQPRKASDGAACHTAHGH
                     RPWQQPEIDLH"
     gene            1093..2142
                     /locus_tag="VNU48_02550"
     CDS             1093..2142
                     /locus_tag="VNU48_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWI10187.1"
                     /translation="MAKRYANILETIGRTPVVRINRLAPPGVNLFVKCESFNPLGSVK
                     DRLAFAVIEAAEQSGELKPGQTVVEATSGNTGIGLAMVCAAKGYPLVLLQAEPFSVER
                     RRLMRFLGARVVITPAAERAVGMINKMEELAVKHGWFMTRQFENEANPDYHSRTTARE
                     IVDDFAGDRLDYWVTGYGTGGTLKGVARVLRRERPETKIVVCEPTDAPLLSSGIEQKR
                     RPDGTPAEPHPSFNPHPMQGWTPDFIPKLTGDAVAMELIDRILQIQGPEAIQASKTLA
                     AKEGIFCGITSGATFAGALRIAAEAPQGANILAMLPDTAERYLSTPLFADIGADMNEE
                     ELAISRSTPGGQLKA"
     gene            2156..3331
                     /locus_tag="VNU48_02555"
     CDS             2156..3331
                     /locus_tag="VNU48_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWI10188.1"
                     /translation="MKIVSERVEAAARVFDAIKDFNFQSRYAERRRQADIADFTFGNP
                     HEMPLPGLVEAIRDGAIPQDMNWFAYKTSEEVPQAFLAGVLARELDLAFEEPPDVALT
                     AGAFAAISVAFRLLLDAGDEVVFCEPGWFCYEPMLLAADAVPRKVPLAKPHFDLDLDA
                     IDAALGARTRLVIVNTPHNPTGRIYGREVLVALAERLERASKRFGRPIFLLSDEPYRR
                     LRFDGRGFVSPAAVYPWTLIAYSYGKVLLAPGQRLGYLAISPRMPADDRQALRDAMFA
                     TQMAIGWCFPNAVMQNALPHIDALSIDVQALTRRRDALSAALAQAGHIVLPPEGTFYL
                     WAKWAGKDPERQWNALADHDVFVMPGRVMNAPDYFRICLTASDAMVAKALPAFAQAA"
     gene            3686..5326
                     /locus_tag="VNU48_02560"
     CDS             3686..5326
                     /locus_tag="VNU48_02560"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HWI10189.1"
                     /translation="MFNWLKQLDRHVPVRYSVWLLCAVGALLCAFTWVAFGIGGWLAL
                     VFMFLVGTGVRDKLQTRHSVLRNYPVLGHVRFLLEFIRPEMRQYFIEGDNEAAPFSRQ
                     QRSLVYQRAKGDADKRPFGTQMDVGAVGYEWINHSLQPTTLATHDFRVLIGAGRAQPY
                     SASLFNISAMSFGALSANAILALNAGAKRGNFAHDTGEGSISRYHRGPDGKGGGGDLI
                     WEIGSGYFGCRHDDGSFSEERFVENATEAQVKMIELKLSQGAKPGHGGVLPGPKVTLE
                     IAAARGVMPWVDCVSPASHAAFRTPIEMMQFIERLRSLSGGKPTGFKLCIGHPWEWFA
                     IAKAMLATGITPDFIVVDGAEGGTGAAPLEFTDHVGAPLQEGLLLVHNTLVGLNLRDK
                     IKLGCAGKVVSAFDIARMLALGADWCNSARGFMFSLGCIQAQSCHTGECPTGVTTQDP
                     QRQKALVVPTKAERVYHFHQQTLMALKELVQAAGLADPGQITAAHIVRRSAEHSVKLL
                     ATLLPFVQPGELLRGEMSQQVFRLYWPVAQASTFGLKE"
     gene            complement(5339..5950)
                     /locus_tag="VNU48_02565"
     CDS             complement(5339..5950)
                     /locus_tag="VNU48_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWI10190.1"
                     /translation="MKLIGSLTSPYVRKVRIVMAEKKLDYQHEIEDVWANDKVMASNP
                     LGKVPCLVLPGGEAIFDSRVIVEYLDTRSPVSRLIPEGSRERIEVRTWEALADGLVDA
                     AILARLEQTWPGRSAEQRSDAWVQRQLHKVEVSINAISTGLADKPWCTGIHLSLADIA
                     VGCALGYLSFRFPQIDWRGRHANLARLADKLDARASFIDTLPP"
     gene            complement(6023..>6894)
                     /locus_tag="VNU48_02570"
     CDS             complement(6023..>6894)
                     /locus_tag="VNU48_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HWI10191.1"
                     /translation="RRVAPYLDLQLVAFPQDGLYRSATAERNLLRALDLGVEVVGGIP
                     HFERTMADGAESVRILCELAAERGLRVDMHCDESDDPLSRHIETLAFHTRRLGLHGRV
                     TGSHLTSMHSMDNYYVSKLLPLMAEAQLHAIANPLINITLQGRHDSYPKRRGMTRVPE
                     LMAAGINVAFGHDCVMDPWYALGSADMLEVAQMGLHVAQMTSQAQMRACFDAVTVNAA
                     RVMGLADYGIEVGHHADFVLLQARDPAEAIRLRASRLIVVRRGAIVARSPAATAALDL
                     PGRPRSVDWTLRR"
BASE COUNT         1096 a         2391 c         2363 g         1044 t
ORIGIN      
        1 tctcgctggg cggcatcgag ggcgacatcc ggcgcatcaa cgtgcgcgcc accgagatcc
       61 agatgggcga ccgctcgacc gtgatcgtgc cgaactccga gttcatcacc aaggtcgtgc
      121 gcaacgtgac gctggccgac ccgctcgggc tggtgcagct caagctgccg gtgccgctgg
      181 gcagcgacgc gcagcaggtg cgcgaactgc tgctgcaggc gttcgccgcc gatgccgagg
      241 tgctcgccac gccggcgccg agcgtgctgc tcgacggcgt cgagcacggc gcgctgctgt
      301 tcaacgcgac cggcttcgtc ggctcgccgc gcagcgccgc gcggatacgc agcgcggtgc
      361 tgttcgatgt gctgaagcgc ctcgccgacg ccggcatcgc cttgtcgagc ccgccgacga
      421 tgctgatcag cgccgccgcg cccgtggcgc cgcaggcgaa cccggccgcg ccggcctgat
      481 caggcgggcg ccgtcacgcg cgggcggctg ccgagcgtcc agcgcgtcag cggccacgcg
      541 acgaccgcga cgaccgcaat gccggccacc atcggccatg ccgtgccgtc gacgaacacg
      601 cccatcaccg cgatcacgac cgcgcccagc acgagctgca gcatgcccat cagggcggcg
      661 gccgcaccgg cgatttcgcc gtggtcggcg agcgccatca ccgaggtcgt cggcacgacc
      721 aggccgagga agccgaagcc gaccagcagc acgacgatca tcggcgcgag gccatcgagg
      781 ccggcgatat tcaatgccgc gagcacgacc atcgagaccg cgaagccgag caccaccagg
      841 atggcggcga tgaccttcag ctcggcgccg cagttccggc agtgctcgaa aatccagctc
      901 gaacacccgc ttgagcagcc gagcccaact cagccgcgca aggcctcgga cggcgctgcg
      961 tgccacaccg ctcacgggca ccggccttgg cagcagcctg aaatagacct gcattgacgc
     1021 cgcccccagg aattcgaata cttccgaaca cgacgtgcga tcccgatcca cctcaaccac
     1081 ccggagagcg ccatggcgaa gcgatatgca aacattctcg agaccatcgg caggacaccg
     1141 gtggtgcgga tcaaccggct ggccccgccg ggggtgaacc tgttcgtcaa gtgcgaatcg
     1201 ttcaacccgc tcggctcggt gaaggaccgc ctcgccttcg cggtgatcga ggcagcggag
     1261 cagtccggcg agctcaagcc cgggcagacc gtggtcgagg cgaccagcgg aaacaccggc
     1321 atcggcctgg ccatggtgtg cgcggcgaag ggttacccgc tggtgctgct ccaggccgag
     1381 ccgttcagcg tcgaacgccg caggctgatg cgcttcctcg gcgcgagggt cgtcatcacc
     1441 ccggctgccg agcgggcggt gggcatgatc aacaagatgg aagagcttgc cgtgaagcac
     1501 ggctggttca tgacgcggca gttcgagaat gaagccaatc cggactacca ctcgcgcacc
     1561 accgcgcgcg agatcgtgga cgacttcgcc ggcgaccggc tggactactg ggtcaccggc
     1621 tacggcaccg gcggcacgct caagggtgtg gcgcgtgtgc ttcggcggga gcggccggaa
     1681 acgaagatcg tcgtttgcga gcccaccgac gcgccgctgc tcagcagcgg catcgagcag
     1741 aagcgcaggc ccgacgggac gcccgccgag ccgcacccga gtttcaaccc gcacccgatg
     1801 caaggctgga cgcccgactt catcccgaag ctcaccggcg acgccgttgc gatggagttg
     1861 atcgatcgca tcttgcagat tcagggcccg gaagcgatcc aggccagcaa gacgctcgcg
     1921 gcgaaggagg gcatcttctg cggcatcacc tctggtgcaa ccttcgccgg cgcgctgcgg
     1981 atcgcggccg aggcgccgca gggcgcgaac atcctggcca tgttgcccga caccgccgag
     2041 cgctacctga gcacgccgct gtttgccgac atcggtgcgg acatgaacga ggaggaactg
     2101 gcgatttcgc gctccacccc aggcggccag ctcaaggcct gaagcgcgca ggcttatgaa
     2161 gatcgtctcc gagcgcgtcg aggcggccgc gcgcgtcttc gacgccatca aggacttcaa
     2221 cttccagtcg cgctacgccg agcggcgcag gcaggcggac atcgcggatt tcaccttcgg
     2281 caacccgcac gagatgccgc tgcccgggct cgtcgaggcc atccgcgatg gcgcgatccc
     2341 gcaggacatg aactggttcg cctacaagac cagcgaagag gtcccgcagg ccttcctggc
     2401 cggggtgctg gcgcgcgaac tcgacctggc cttcgaggag ccgccggacg tggcgttgac
     2461 cgccggcgcg ttcgccgcca tctcggtcgc cttccggttg ctgcttgacg ccggtgacga
     2521 ggtagtcttc tgcgagcccg gttggttctg ctacgaaccg atgctgctcg ccgccgacgc
     2581 cgtgccgcgc aaggtgccgc tggccaagcc gcacttcgat ctcgacctgg acgcgatcga
     2641 cgcggcgctc ggcgcgcgca cgcggctcgt catcgtgaac accccgcaca acccgaccgg
     2701 gcgcatctac gggcgggagg tgctggtggc gctggcggaa cggctcgaac gagcgtccaa
     2761 gcgctttggc cggccgatct ttctgctgtc cgacgaaccc taccggcgcc tgcgcttcga
     2821 cggccgtggc ttcgtgagcc cggcggccgt gtacccgtgg acgctgatcg cgtacagcta
     2881 cggcaaggtg ctgctcgcgc caggccagcg cctgggctat ctcgccatct cgccgcgcat
     2941 gccggcggac gaccggcagg ccttgcgcga cgcgatgttc gccacccaga tggcgatcgg
     3001 ctggtgcttc cccaatgcgg tcatgcagaa cgcgctgccg cacatcgatg cgctgagcat
     3061 cgatgtgcag gcgctgaccc ggcgacgcga cgcgctgagc gccgcgctcg cccaggccgg
     3121 ccatatcgtg ctgccgccgg aggggacctt ctacctctgg gcgaaatggg ccggcaagga
     3181 ccccgagcgg cagtggaacg cgcttgcgga ccacgacgtg ttcgtcatgc cgggccgcgt
     3241 gatgaacgcg cccgactact tccggatctg cctcaccgca tccgatgcga tggtggcgaa
     3301 ggccttgcct gcgttcgcgc aggcggcgtg agcggatgtc gccgaaacgt gttgctcgag
     3361 ctgctgcttc ttgttggtga tctcgtcgac gtcttgcgcc agcgcgctct cggccccagt
     3421 gatcgaaggt tccaaattca ggcacccgaa ccggacgtca tgagcacccg caaatggccg
     3481 attgcgggtc ttcactcaac attcctcctg gcaacctgac cagggcaccg ccggcgatgg
     3541 ctgcgcggcc attgcgccgc ctgcgcacgc tgccggccgg cgtgcactga tccgctgaac
     3601 tggcggggta ttcgggcatt cccgcagcgg acacggtcgc gcggcgggcc tacgatcgcg
     3661 ccaaacacgt gaggcgctga cgttcatgtt caactggctc aagcagctcg atcgtcacgt
     3721 gccggtgcgc tattcggtgt ggctgctgtg cgcggtgggc gcgctgctgt gcgcgttcac
     3781 ctgggtcgcg ttcggcatcg gcggctggct cgcgctggtg ttcatgtttt tggtcggcac
     3841 cggcgtgcgc gacaagctgc agacgcgcca ctcggtgctg cgcaactacc cggtgctcgg
     3901 ccatgtccgc ttcctgctcg aattcatccg ccccgagatg cgccagtatt tcatcgaggg
     3961 ggacaacgag gccgcgccgt tctcgcgcca gcagcgctcg ctggtgtacc agcgcgcgaa
     4021 gggcgacgcc gacaagcggc cgttcggcac gcagatggac gtgggcgcgg tcggctacga
     4081 gtggatcaac cactcgctgc agccgaccac actggccacc cacgactttc gcgtgctgat
     4141 cggcgcgggc cgcgcgcagc cctacagcgc gagcctcttc aacatctcgg cgatgagctt
     4201 cggcgcgctc agcgcgaacg cgatcctggc gttgaacgcc ggcgccaagc gcggcaactt
     4261 cgcgcacgac accggcgagg gctcgatcag ccgctaccac cgcggtcccg atggcaaggg
     4321 cgggggcggc gacctgatct gggagatcgg ctcgggctac ttcggctgcc gccacgacga
     4381 cggctcgttc agcgaggaac gcttcgtcga gaacgcgact gaagcgcagg tcaagatgat
     4441 cgagctcaag ctcagccagg gcgccaagcc cggccacggc ggcgtgctgc cggggcccaa
     4501 ggtcacgctc gagatcgccg cggcgcgcgg cgtgatgccg tgggtcgatt gcgtctcgcc
     4561 ggcgagccac gccgccttca ggacgccgat cgagatgatg cagttcatcg agcggctgcg
     4621 cagcttgtcg ggcggcaagc cgaccggctt caagctgtgc atcggccacc cctgggagtg
     4681 gttcgcgatc gccaaggcga tgctcgccac gggcatcacg cccgacttca tcgtcgtcga
     4741 cggcgccgag ggcggcaccg gcgcggcgcc gctcgaattc accgaccacg tcggcgcgcc
     4801 gctgcaggaa gggctgctgc tggtgcacaa cacgctggtc gggctgaacc tgcgcgacaa
     4861 gatcaaactc ggctgcgccg gcaaggtcgt cagcgcgttc gacatcgcgc gcatgctggc
     4921 gctgggtgcg gactggtgca actcggcgcg cggcttcatg ttctcgctcg gctgcatcca
     4981 ggcacagtcg tgccacaccg gcgagtgccc gaccggcgtg acgacgcagg acccgcagcg
     5041 ccagaaggcg ctggtcgtgc cgaccaaggc cgagcgcgtc taccacttcc atcagcagac
     5101 gctgatggcg ctgaaggagc tggtgcaggc cgcgggcctc gccgacccgg gccagatcac
     5161 cgccgcgcac atcgtgcggc gcagtgccga gcacagcgtg aagctgctcg ccacgctgct
     5221 gccgttcgtg cagccgggcg agctgctgcg cggcgagatg tcgcagcagg tgttccgcct
     5281 ctactggcct gtggcccagg cctcgacctt cggcctcaag gaatgacgac ccgccggatc
     5341 agggcggcag cgtgtcgatg aaactcgcgc gcgcgtcgag cttgtcggcc aggcgggcga
     5401 ggttcgcgtg ccggccgcgc cagtcgatct gcgggaagcg aaagctcagg tagccgagcg
     5461 cgcaacccac ggcgatgtcg gccagcgaca ggtggatgcc ggtgcaccag ggcttgtcgg
     5521 ccagcccggt gctgatcgcg ttgatcgaga cctcgacctt gtgcagctgg cgctggaccc
     5581 acgcgtcgct gcgctgctcg gcgctgcgcc cgggccaggt ctgctcgagc cgcgcgagga
     5641 tcgccgcgtc gacgaggccg tcggccagcg cctcccaggt gcgcacctcg atgcgctcgc
     5701 ggctgccttc ggggatcagc cggctcaccg gcgagcgcgt gtccaggtac tcgacgatca
     5761 cgcgcgaatc gaagatcgcc tcgccgcccg gcagcaccag gcacggcacc ttgccgagcg
     5821 gattcgacgc catgaccttg tcattggccc agacgtcctc gatctcgtgt tggtagtcga
     5881 gcttcttctc ggccatcacg atgcgcacct tgcgtacgta gggactggtg agcgaaccga
     5941 tgagtttcat aatcgttgta ccgtccctga cgccatggcg ctggctgtgg cgattttagc
     6001 ggcgcgcctc gcgcgcagcg gctcagcggc gtagcgtcca gtccacactt cgcggccggc
     6061 cgggcaggtc gagcgccgcg gtcgcggccg gcgagcgcgc gacgatggcg ccgcggcgca
     6121 ccacgatgag gcggctcgcc cgcaggcgga tcgcttcggc cgggtcgcgc gcctgcagca
     6181 gcacgaagtc cgcgtgatgg cccacctcga tgccgtagtc ggccagcccc atcacgcgcg
     6241 ccgcattgac ggtgacggcg tcgaagcagg cccgcatctg cgcctgcgag gtcatctgcg
     6301 cgacatgcag ccccatctgc gcgacttcga gcatgtcggc gctgccgagc gcataccacg
     6361 ggtccatcac gcagtcgtgg ccgaacgcga cgttgatgcc ggccgccatc agctcgggca
     6421 cgcgcgtcat gccgcggcgc ttcggatagc tgtcgtggcg gccctgcaag gtgatgttga
     6481 tcagcgggtt cgcgatcgca tggagctgcg cctcggccat cagcggcagc agctttgaca
     6541 cgtaatagtt gtccatcgaa tgcatcgacg tgaggtgcga gccggtgacg cggccgtgca
     6601 gcccgaggcg ccgggtgtgg aacgcgaggg tctcgatgtg gcgcgacagc gggtcgtcgc
     6661 tctcgtcgca atgcatgtcc acgcgcaggc cgcgctcggc ggcgagttcg cacaggatgc
     6721 gcaccgactc ggcgccatcg gccatcgtgc gctcgaagtg cgggatgccg ccgaccacct
     6781 cgaccccgag gtcgagcgcg cgcagcaggt tccgctcggc ggtggcggag cggtacagcc
     6841 cgtcctgcgg gaaggcgacc aactggaggt cgagataggg cgcgacccgg cgct
//
LOCUS       DATMJB010000115         4920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_872574, whole genome shotgun sequence.
ACCESSION   DATMJB010000115 DATMJB010000000
VERSION     DATMJB010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872574"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2665
                     /locus_tag="VNU48_02575"
     CDS             <1..2665
                     /locus_tag="VNU48_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWI10192.1"
                     /translation="AAIAGAAQPMQRDYVWPYQLHASIGPSCALADWRRDTMTVWTGS
                     QNPHVLRADLALLTGLAETAIDVIRMEASGCYGRNGADDVAADAALLSRAVGAPVRVQ
                     LSREQEHLWEPKGAAQLMQVNGGLAADGRAAAYDFQTSYPSNGAPTLALLLTRKVDPE
                     TTAYEMGDRTAVPPYAYANLRVVANDMVPILRASWLRGVSALPNSFAHESYVDELASA
                     AGVDPVEFRLRHLTDPRAIDLVKATAARAGWRAHTQPQQQRADGAILKGQGFAYARYL
                     HSKFPGFGAAWSAWVADVEVNRVTGEVHVSRVVVGQDAGLTINPAGVQHQTHGNVLQT
                     TSRALKESLPLDPRTNVPVAREWGSYPILNFREVPVVEVLMMPRPGDPPLGVGESSSV
                     PGTAAIANAIFDATGVRFRAPPFTPEVVRAALNPLPAPDAAATTAPLVAGAAPTRPRD
                     AIWPKRRGLWARSAALAAGAIGVGVALLGWRAAIAPIAPGSGNVYTAATIAKGRVLAA
                     AGNCIVCHTAPGGTPNAGGRAMATPFGTVYTTNLTPDPATGIGTWSFSAFQRAMREGI
                     SRDGHHLYPAFPYTAFTRMTDEDLTALYAYLMAQAPVAAAVPDTALAFPFNLRPLMAA
                     WNALYLAPGPSAAVATRSAEWMRGAYLVDGVGHCGACHSARNALGAEQGGSAYLGGAM
                     VDGWEAPPLTSLSHAPVRWTGDELYRYLRFGHSAEHGSASGPMAPVVRQLAQLPEADV
                     RAMAVYLASFNTASAGAPTAAALVAQSGTRAAAALPGPAQRLFTTACGACHHDGAGPQ
                     LLGQNIPLALNTKLHSARPDNLLRVMLEGVREPASRELGFMPAFRDAFDDAQLAQLAA
                     HLRERFAPDKPAWLDLEAASARVRAAPSAPH"
     gene            complement(2742..3377)
                     /locus_tag="VNU48_02580"
     CDS             complement(2742..3377)
                     /locus_tag="VNU48_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWI10193.1"
                     /translation="MKFSRSLLSAAAAIGLSAMALPALALDYTLDNGGSAFATPGDYG
                     TVSLTQDGTSVDFSITLAPTFSFVTTGNANSHVLFGFNATGVVAGDISGITAGNGGTF
                     SVGAPATGSPFGDFTFGIYCTSCKNGGPGATTPPLTFTVANATLADFAFLSTGGNPNA
                     FFTADVISGATTGQVGATGTTPPVPEPGTYALMFAGLGVVGFMARRRKQQA"
     gene            3585..4508
                     /locus_tag="VNU48_02585"
     CDS             3585..4508
                     /locus_tag="VNU48_02585"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HWI10194.1"
                     /translation="MSMADRDGKIWMDGAMVDWRDAKIHVLTHTLHYGCGAFEGVRAY
                     NTVNGTAIFRLREHTERLFNSAKILRMKIPFSFEQVMQAQLDVVRQNRLESCYLRPLT
                     WIGSEKLGVSPKGNQVHLMVAAWPWGAYLGEDGMKRGIRVKISSYTRHHVNITMTQAK
                     TVSNYTNSILANMEATDDGYDEAMLLDANGFVSEGAGENLFVVKNGVIYTPDLSAGAL
                     NGITRNTVFAICADLGLKVVEKRITRDEVYICDEAFFTGTAAEVTPIRELDRIELGSG
                     SRGPITEKIQSAFFDIVNGRNPKYAEWLSKV"
     gene            4514..4723
                     /locus_tag="VNU48_02590"
     CDS             4514..4723
                     /locus_tag="VNU48_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HWI10195.1"
                     /translation="MDSSKASASVEVTAKDLQGPGVVFCPNPKMTLWSTHPRVFIDVV
                     TTGEGKCPYCGTVYRLKAGEKVHAH"
     gene            4728..>4920
                     /locus_tag="VNU48_02595"
     CDS             4728..>4920
                     /locus_tag="VNU48_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HWI10196.1"
                     /translation="MSRSLIVAPQWIGDAVMSEPLLARLAARGEQLAVAALPWVAPVY
                     RAMPQVAEVIELPFAHGRLD"
BASE COUNT          775 a         1697 c         1735 g          713 t
ORIGIN      
        1 cgccgcgatc gccggcgccg cgcagccgat gcagcgcgac tacgtctggc cgtaccagct
       61 gcacgcgtcg atcgggccgt cgtgcgcgct ggccgactgg cgccgtgaca cgatgaccgt
      121 ctggacgggc tcgcagaacc cgcacgtgct gcgcgccgat ctcgcgctgc tgaccggcct
      181 ggccgagacc gcgatcgacg tgatccggat ggaggcttcg ggctgctacg gccgcaacgg
      241 cgccgacgac gtggcggccg acgcggcgct gctgtcgcgc gcggtcggtg cgccggtgcg
      301 ggtgcagctg tcgcgcgagc aggagcatct gtgggagccg aagggcgcgg cgcagctgat
      361 gcaggtcaac ggcggcctgg ccgccgacgg ccgcgccgcg gcctacgact tccagacctc
      421 ctatccgtcg aacggcgcgc cgaccctggc gctgctgctg acgcgcaagg tcgaccccga
      481 aacgaccgcc tacgagatgg gcgaccgcac cgcggtgccg ccgtatgcgt acgcgaacct
      541 gcgcgtggtc gcgaacgaca tggtgccgat cctgcgcgcc tcgtggctgc gtggcgtgtc
      601 ggcgctgccg aactcgttcg cgcacgagtc gtatgtcgac gagctcgcga gcgcggcggg
      661 cgtggacccg gtcgagttcc ggctgcgcca cctgaccgac ccgcgcgcga ttgatctcgt
      721 caaggccacc gccgcgcgcg ccggctggcg cgcacacacg cagccgcagc agcagcgcgc
      781 cgatggggcg atcctgaagg gccagggctt cgcctatgcg cgctacctgc acagcaagtt
      841 ccccggcttc ggcgccgcgt ggtcggcctg ggtcgccgac gtggaggtga atcgcgtgac
      901 cggcgaggtg cacgtgagtc gcgtcgtcgt cggccaggac gcgggcctga cgatcaaccc
      961 ggccggcgtg cagcaccaga cccacggcaa cgtgctgcag accacctcgc gcgcgctgaa
     1021 ggaatcgctg ccgctggacc cgcgcacgaa cgtgccggtg gcgcgcgaat ggggcagcta
     1081 cccgatcctg aatttccgcg aggtgccggt cgtcgaggta ctgatgatgc cgcggccggg
     1141 cgacccgccg ctgggcgtgg gcgagtcgtc gtcggtgccg ggcaccgcgg cgatcgcgaa
     1201 cgcgatcttc gacgcgaccg gcgtgcgctt tcgcgcgccg ccgttcacgc ccgaggtcgt
     1261 gcgcgccgcg ctgaacccgc tgccggcgcc ggatgcggcg gcgacgaccg cgccgctggt
     1321 ggccggcgcg gcgccgacgc gaccacgcga tgcgatctgg ccgaagcggc gggggttgtg
     1381 ggcgcgcagc gccgccctgg cggccggcgc gatcggcgtc ggcgtcgcac tgctcggctg
     1441 gcgcgccgcg atcgcgccga tcgcgcccgg cagcggcaat gtctacaccg ctgccacgat
     1501 tgccaagggc cgcgtgctcg ccgcggccgg caactgcatc gtctgccata ccgcgcctgg
     1561 cggcacgccc aatgccgggg gccgcgcgat ggccacaccg ttcggcacgg tctacacgac
     1621 caacctgacg cccgacccgg ccaccggcat cggcacctgg tcgttcagcg cgttccagcg
     1681 cgcgatgcgc gaaggcatct cacgcgacgg ccaccacctg tacccggcgt tcccgtacac
     1741 cgcgttcacg cgaatgaccg acgaagacct gaccgcgctc tacgcctacc tgatggcgca
     1801 agcgccggtc gccgccgcgg tgcctgacac ggcgctggcg ttcccgttca acctgcggcc
     1861 gctgatggcc gcgtggaacg cgctctacct cgcgccgggc ccgagcgctg ccgtcgcgac
     1921 gcgatcggcc gaatggatgc gcggcgccta cctcgtcgac ggcgttggcc actgcggcgc
     1981 ctgccacagc gcgcgcaacg cgctcggcgc cgaacagggc ggaagcgctt acctcggcgg
     2041 cgcgatggtc gacggttggg aagcgccgcc gctcacatcg ctcagccacg cgccggtgcg
     2101 ctggaccggc gacgagctgt atcgctacct gcgcttcggc cactcggcgg agcatggcag
     2161 cgcgagcggc ccgatggcgc cggtggtgcg gcagctcgct cagctgccgg aggccgacgt
     2221 gcgcgcgatg gccgtctacc tcgcgtcgtt caacaccgcg tcggccgggg caccgaccgc
     2281 tgccgcgctg gtggcgcaaa gcggcacgcg cgccgccgcc gcactgcccg gaccggcgca
     2341 gcgcctgttc acgaccgcct gcggcgcctg ccaccacgac ggcgcgggcc cgcagctgct
     2401 gggccagaac atcccgctcg cgctcaacac caagctgcac agcgcgcgcc ccgacaatct
     2461 gctgcgtgtg atgctcgagg gcgtgcgcga accggcgtca cgcgagctcg gtttcatgcc
     2521 agcgttccgc gatgcgttcg acgatgcgca gctcgcgcag cttgcggcgc atctgcgtga
     2581 acgcttcgcg cccgacaagc cggcgtggct cgacctcgaa gccgcgagtg cgcgtgtgcg
     2641 cgccgcgccg tccgcaccgc actgacttga gaggctctgc cgctgcgccg gacgagaaaa
     2701 agccccgacg agcggggctt gatcgttgag gcgcaggacg ctcaggcttg ctgcttgcgg
     2761 cggcgcgcca tgaagccgac gacacccagg ccggcgaaca tcagcgcgta ggtgccgggc
     2821 tccgggaccg ggggcgtcgt gccggtggcg ccgacctgac cggtggtggc gccagagatg
     2881 acatccgccg tgaagaacgc gttcggattg ccgcccgtgc tcaggaaagc gaagtccgcc
     2941 agcgtggcat tggccacggt gaacgtgagc ggaggggtcg tcgcgcccgg cccgccgttt
     3001 ttgcagctcg tgcagtagat gccgaaggtg aaatcaccga acgggctgcc ggtggcggga
     3061 gcccccaccg agaacgtgcc accgttgccg gccgtgatgc ccgagatgtc gccggcgacc
     3121 acgccggtgg cattgaagcc gaaaaggacg tggctgttcg cgttaccggt cgtcacgaag
     3181 ctgaacgtgg gtgccagcgt gatgctgaag tcgacgctcg tgccgtcttg ggtcagcgag
     3241 accgtgccgt agtcgccggg ggttgcgaac gcggagccgc cgttgtcgag ggtgtagtcg
     3301 agcgcgagcg ctggcagcgc catagccgac agcccgatgg ccgctgcggc gctgagaaga
     3361 ctgcgagaaa acttcatgag gaactcctat gaaaaatgat ggccgatcgc tgcatccgtg
     3421 gatgggtatc gattcgggct gaacgtattc tgcgtctagg cgacggcaca cggtcccccc
     3481 gggtcgcggg gttttcgctg taacgcccgg taaacaagcg cccgccgcgt tgcgcaaaac
     3541 cgggtcgata gaatccgttc tcaacctgca tcatggagag cgccatgtcg atggccgacc
     3601 gcgacggaaa gatctggatg gatggcgcga tggtcgactg gcgcgacgcc aagatccacg
     3661 tgctgaccca cacgctgcat tacggctgtg gcgctttcga aggcgtgcgc gcttacaaca
     3721 cggtgaacgg cacggcgatc tttcgcctcc gcgaacacac cgagcggctg ttcaacagcg
     3781 cgaagatttt gcgcatgaag atcccgttca gcttcgagca ggtgatgcag gcgcagctgg
     3841 acgtggtgcg ccagaacagg ctcgaatcct gttacctgcg cccgctgacc tggatcggct
     3901 ccgagaaact cggcgtgtcg ccgaagggca atcaggtgca cctgatggtc gccgcctggc
     3961 cctggggcgc gtatctcggc gaggacggca tgaagcgcgg gatccgcgtg aagatctcca
     4021 gctacacgcg ccaccacgtc aacatcacga tgacccaggc caagacggtc agcaactaca
     4081 ccaattcgat cctcgccaac atggaagcca ccgacgacgg ctacgacgag gcgatgttgc
     4141 tcgacgcgaa cggcttcgtc agcgaaggtg cgggcgagaa cctgttcgtc gtcaagaacg
     4201 gcgtcatcta cacgcccgac ctgtcggccg gtgcgctcaa cggcatcacc cgcaacaccg
     4261 tgttcgcgat ctgcgccgac ctcgggctga aggtggtcga gaagcgcatc acgcgcgacg
     4321 aggtctacat ctgcgacgag gccttcttca ccggcaccgc cgccgaggtc acgccgatcc
     4381 gcgaactcga ccgcatcgag ctcggctcgg gctcgcgggg gccgatcacc gagaagatcc
     4441 agagcgcgtt cttcgacatc gtgaacggcc gcaatccgaa gtacgccgaa tggctcagca
     4501 aggtgtgatg gcgatggaca gcagcaaagc cagcgcgagc gtcgaagtca cggcgaaaga
     4561 cctgcaaggc ccgggcgtcg tgttctgccc gaacccgaag atgacgctgt ggagcacgca
     4621 cccgcgcgtg ttcatcgatg tggtcacgac cggcgagggc aagtgcccgt attgcggcac
     4681 cgtgtatcgc ctgaaggcgg gcgagaaggt ccacgcccat tgacggtgtg agccgctcgc
     4741 tgatcgttgc gccgcagtgg atcggcgacg cggtgatgtc cgagccgctg ctcgcgcggc
     4801 tcgcggcgcg cggcgagcag ctcgcggtcg cggcattgcc gtgggtcgcg ccggtgtacc
     4861 gggcgatgcc gcaggtcgcc gaggtcatcg agctgccgtt cgcgcacggc cggctcgact
//
LOCUS       DATMJB010000116         2550 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_754002, whole genome shotgun sequence.
ACCESSION   DATMJB010000116 DATMJB010000000
VERSION     DATMJB010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754002"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1398)
                     /locus_tag="VNU48_02600"
     CDS             complement(<1..1398)
                     /locus_tag="VNU48_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HWI10197.1"
                     /translation="MSATPPTFWHRLDRTLARVRMHRRPLSLLIDGLVIALCWNATYL
                     FRLGFERWLDARPSYDVWVMSGIVAVYLGVFVLAGVPRGMWRFSGFGEVKRLTLACLA
                     SGLLCAVVVLMAQLTKVPRAVLALHPLISLIGLCMVRVAYRMLYEHARSQITGSDTEV
                     RRAIVMGAGEAAKLLLASIHQQGWIVLGLLDDDPAKRGARIGGVPVLGRLADVARPEI
                     RAEATHVIVALPGATVAQRRKAIDLAAATELPVLTVPSASELQDGSARVERVRSVEPE
                     DLLGRESVQLDEAGIAELISGKTVLVTGAGGSIGSELCRQIARYGPARLVLYELSEFA
                     LYQIEQQLGELHPHLPLVRLMGDVKNLAHLRATFMAQRPHLILHAAAFKHVPLMEDEN
                     AWAALQNNTLGTWNAATAAAEAGVQRFVLISTDKAVNPTNVMGATKRAAEMVVSRMAR
                     AGHATRYAAVRFGNVL"
     gene            complement(1395..1991)
                     /locus_tag="VNU48_02605"
     CDS             complement(1395..1991)
                     /locus_tag="VNU48_02605"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWI10198.1"
                     /translation="MAKRAFDLVCAALGLVLLAPLLLGIALWIKLDSRGPVFFRQQRV
                     GRRGVPFRIHKFRTMVADAPALGAPITVGADPRITRAGRFLRRAKLDELPQLIDVLVG
                     TMSLVGPRPELPRYVALYPAELRDKVLSVRPGITDPASIEYRDEAALLARAADPERAY
                     VEQVLPAKLRCSVRYVEQRSMRGDLRLIGATLGALWAR"
     gene            complement(2000..>2550)
                     /locus_tag="VNU48_02610"
     CDS             complement(2000..>2550)
                     /locus_tag="VNU48_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWI10199.1"
                     /translation="LLAERMRTMSLHGLSKDAWDRYSIGGTWDYKIVAPGFKYNLTDI
                     AAAMGIHQLRRARAFHERRAQIAQAYDAGFAGLPLTTPPGPAAGDLHAWHLYPLRLSD
                     AAPIDRDVLIERLFAAGIGCSVHYIPLHLQPYWRDRYALRAGQFPHSQHAYERMLSLP
                     IYTRMTDADVRRVVDAVAAALV"
BASE COUNT          380 a          927 c          911 g          332 t
ORIGIN      
        1 cagcacgttg ccgaagcgca ccgccgcgta gcgggtggcg tgcccggcgc gcgccatgcg
       61 cgacaccacc atctcggcgg cccgcttggt cgcgcccatc acgttggtcg ggttcaccgc
      121 cttgtcggtg gagatgagca cgaagcgctg cacgcccgcc tcggccgcgg cggtggccgc
      181 gttccaggtg cccagcgtgt tgttctgcag cgcggcccag gcgttctcgt cctccatcag
      241 cggcacatgc ttgaacgccg cggcatgcag gatcaggtgt gggcgctgcg ccatgaacgt
      301 ggcgcgcaga tgcgcgaggt tcttcacgtc gcccatcagc cgcaccagcg gcaggtgcgg
      361 gtgcagttcg cccagctgct gctcgatctg gtacagcgcg aactcgctca gctcgtacag
      421 caccagccgc gccgggccgt agcgggcgat ctggcggcac agctccgagc cgatcgaccc
      481 gccggcgccg gtcacgagca ccgtcttgcc ggagatcagc tcggcgatgc cggcctcgtc
      541 gagctgcacc gactcgcggc cgagcaggtc ctcgggttcg acgctgcgca cccgctcgac
      601 gcgcgcgctg ccgtcctgca gttcgctcgc ggacggcacc gtgagcaccg gcagttcggt
      661 ggccgcggcc agatcgatgg ccttgcggcg ctgcgcgacc gtggcgccgg gcagcgccac
      721 gatcacgtgg gtggcctcgg cccgaatctc gggtcgggcc acgtcggcca gccggcccag
      781 caccggcacg ccgccgatgc gcgcaccgcg cttggccggg tcgtcgtcga gcaggcccag
      841 cacgatccag ccctgctggt ggatgctggc cagcagcagc ttggccgctt cgcccgcgcc
      901 catcacgatc gcgcgccgca cctcggtgtc gctgccggtg atctgcgagc gcgcgtgctc
      961 gtacagcatg cggtaggcca cgcgcaccat gcacaggccg atcaggctga tcagcgggtg
     1021 cagcgccagc accgcgcgcg gcaccttcgt gagctgcgcc atcagcacca ccaccgcgca
     1081 cagcagcccg ctggccaggc acgcgagcgt caggcgcttg acctcgccga agcccgagaa
     1141 gcgccacatg ccgcgcggca cgccggcgag cacgaacacg cccaggtaga ccgcgacgat
     1201 ccccgacatc acccagacgt cgtagctcgg ccgcgcatcc agccagcgct cgaagccgag
     1261 gcggaacagg taggtcgcgt tccagcacag cgcgatcacc aggccgtcga tcagcagcga
     1321 cagcggccgg cggtgcatgc gcacgcgcgc gagcgtgcga tcgagccggt gccagaaggt
     1381 gggcggcgtg gcgctcatcg tgcccacagt gcgccgagcg tcgcgccgat cagccgcagg
     1441 tcgccgcgca tcgaccgctg ctccacgtag cgcaccgagc aacgcagctt cgccggcagc
     1501 acctgctcga cataggcgcg ctcgggatcg gcggcgcgcg cgagcagcgc ggcctcgtcg
     1561 cggtattcga tcgacgccgg gtcggtgatg ccgggccgca ccgacagcac cttgtcgcgc
     1621 agctcggccg ggtacagcgc cacgtagcgc ggcagctcgg gccgcgggcc gacgaggctc
     1681 atcgtgccga ccagcacgtc gatcagctgc ggcagttcgt cgagcttggc gcgccgcagg
     1741 aagcggccgg cgcgcgtgat gcgcggatcc gcgccgaccg tgatcggcgc gccgagcgcc
     1801 ggcgcgtcgg ccaccatcgt gcggaacttg tggatgcgaa acggcacgcc gcgccggccg
     1861 acgcgctgct gccgaaagaa caccggcccg cgcgaatcga gcttgatcca cagcgcgatg
     1921 cccagcagca gcggcgcgag cagcaccagc ccgagcgcgg cgcagacgag gtcgaacgcg
     1981 cgcttggcca cggccggcat cagacgagtg ccgccgcgac cgcatccacc acgcgccgca
     2041 cgtcggcatc ggtcatgcgc gtgtagatcg gcaggctgag catgcgctcg taggcgtgct
     2101 ggctgtgcgg gaactggccg gcgcgcagcg cgtagcggtc gcgccagtag ggctgcaggt
     2161 gcagcgggat gtagtgcacg ctgcagccga tgccggcggc gaacagccgc tcgatcagca
     2221 catcgcggtc gatcggcgcc gcgtcagaca agcgcagcgg atacagatgc caggcgtgca
     2281 ggtcgccggc cgccggcccc ggcggcgtgg tcagcggcag gccggcaaag cccgcgtcgt
     2341 aggcctgcgc gatctgcgcg cgccgttcgt ggaacgcgcg ggcgcgccgc agttggtgga
     2401 tgcccatcgc cgccgcgatg tcggtcaggt tgtacttgaa gcccggcgcg acgatcttgt
     2461 agtcccaggt gccgccgatg gagtagcggt cccacgcgtc cttcgagagg ccgtgcagcg
     2521 acatcgttcg catccgctcg gcgagaagcg
//
LOCUS       DATMJB010000117         3908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_406985, whole genome shotgun sequence.
ACCESSION   DATMJB010000117 DATMJB010000000
VERSION     DATMJB010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406985"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..98
                     /locus_tag="VNU48_02615"
     CDS             <1..98
                     /locus_tag="VNU48_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWI10200.1"
                     /translation="HEDMILRGRRILADQMEFLLASVPRNSRLHG"
     gene            complement(267..609)
                     /locus_tag="VNU48_02620"
                     /pseudo
     CDS             complement(267..609)
                     /locus_tag="VNU48_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(631..761)
                     /locus_tag="VNU48_02625"
                     /pseudo
     CDS             complement(631..761)
                     /locus_tag="VNU48_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654554.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            complement(785..1948)
                     /locus_tag="VNU48_02630"
     CDS             complement(785..1948)
                     /locus_tag="VNU48_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HWI10201.1"
                     /translation="MPAAKPQEPLLAKAHELARTATQLAGLPLARELRTLLRGMNSYY
                     TNRIEGQHTRPHEIEQALRRDFSHDKNLAARQRLAVAHIEAEAAIEARYADDAGATRL
                     YSVDAVQDLHRELFARLPATDLVTPEGEPIVPGQLRLRDVRVGEHVAPTHASLAAFLQ
                     RWAKFYRGVRRGEAALVALAAAHQRLGWVHPFIDGNGRVMRLHTHAVLSALGYTNGLW
                     SPLRGFARSTERYYALLAAADAPRHGDLDGRGNLSERALVAWVDYVLDVCLDQVRFMT
                     KLLDLGAMERRIAACLAFEEETLRSGVRREALRPLHYLFLTGGELERGEFKRMMGLGD
                     RTAVTALTALVKRGLLKSDTSQGKLRFGIPLHALRFYFPALWPEAEADAHDTA"
     gene            complement(2084..2299)
                     /locus_tag="VNU48_02635"
     CDS             complement(2084..2299)
                     /locus_tag="VNU48_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HWI10202.1"
                     /translation="MTNEQLEQRLTDLEIKASFAEDLVDHLNALVARQQTQIDWLLRE
                     VAALRQQAPAGEGGAPRSLRDELPPHY"
     gene            complement(2439..2693)
                     /locus_tag="VNU48_02640"
     CDS             complement(2439..2693)
                     /locus_tag="VNU48_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10203.1"
                     /translation="MIELHAMMQAFRDYRITGGVPVLRQVESLYYHQTVDSEFKAFLT
                     DHEVGKRVLQALQAAAAIITIALHMPELSHKVTLLLSGPT"
     gene            complement(2809..2886)
                     /locus_tag="VNU48_02645"
     tRNA            complement(2809..2886)
                     /locus_tag="VNU48_02645"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2849..2851),aa:Ile,seq:cat)
     gene            complement(2920..3900)
                     /gene="hrcA"
                     /locus_tag="VNU48_02650"
     CDS             complement(2920..3900)
                     /gene="hrcA"
                     /locus_tag="VNU48_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HWI10204.1"
                     /translation="MLKALVERYIADGQPVGSRTLSRASGLELSPATIRNVMADLEDL
                     GLIASPHTSAGRIPTARGYRLFVDTMLTAQPFAAVAAPQLPPDQPQRVIANAAQLLSN
                     LSHFVGVITAPRKASVFHHIEFMRLSERRVLMILVAPDGDVQNRVIFTAQDYTQSQLI
                     EASNYLTAHYRGLTIEAVRERLKTEVDALRGEIAALMQAAVQAGSEAHADSQEQVVIS
                     GERNLLAVQDFGSDMGSLRKLFDVFEQKTQLMRLLEVSSRAEGVRIYIGGESMVVPFE
                     ELSVVSAPYEVDGQIVGTLGVIGPTRMAYDRMIQIVDITSKLVSNALSTK"
BASE COUNT          630 a         1293 c         1296 g          689 t
ORIGIN      
        1 tgcacgagga catgatcctg cgcggccgcc gcatcctggc cgaccagatg gagttcctgc
       61 tcgcgagcgt gccgcggaac agccgattgc acggatagaa aagcaagcgg ggtcggcgcg
      121 ccagcgccga cgaccggcta ctgcggaatc agccgccaga cgccgcctgt gagctgcatc
      181 aactcgcggc cgcgccggtc gaagccggaa tgatcggccg ccgacatgtt gtagaccccc
      241 tgcgtgccga ccagctcgcg tgtgccttcg agggcgtcgc gcagcgccgc catcgagttc
      301 ggcgagcccg agggcccgat gatcgcgtcg accttgtcga tcagcagctt cttgatcagc
      361 gcgacggtcg gggtcgagtc cgaggcgtcg tcgagcacgg tgtactcgac gtcgagattg
      421 ccgatcttct tcggcattct tcggcagcag cgcggcgctg ttcttctgcg ggatgccgac
      481 cgcggcggtc gggccggtgg ccgacgcgat cacgccgacc ttcatctgag cctgggcggc
      541 gcaggccgcc agcgcggcca gcgccaccag cgccagcgtc acgagtcggg cgggcaactg
      601 caaggtcatc gtcgagtctc cggtcgcacg tcagcgcgcg ccgaactgcg cgttctcgaa
      661 caggtccttg aactcgctcg gcgccagtac tgcggcggcg cctgcacctg ggcacccagc
      721 gccgcggccg cgtgccaggg ccagcgcggg tcgtagagga cctcgtcgac cgatggcacc
      781 tcgctcacgc agtgtcgtgc gcgtcggctt cagcctcggg ccagagcgcc gggaagtaaa
      841 agcgcagcgc gtgcagcggg atgccgaagc gcagcttgcc ttgtgatgta tccgatttca
      901 gcagaccgcg cttgaccagc gcggtcagcg cggtgacggc ggttcgatcg cccaggccca
      961 tcatccgttt gaactctccg cgttcgagtt caccgcccgt gagaaagaga tagtgcaatg
     1021 gccgcagcgc ctcgcggcgc acgccggagc gcaaggtctc ttcctcgaac gcaaggcagg
     1081 ccgcgatgcg gcgctccatc gcgccgagat cgagcaactt cgtcatgaag cggacctgat
     1141 cgaggcagac atcgagcacg tagtcgaccc aggccaccag tgcgcgctcg ctcaggttgc
     1201 cgcggccgtc gagatcgcca tggcgcgggg catcggcagc cgcgagcagc gcgtagtagc
     1261 gctcggtcga acgcgcgaag ccccgcagcg gcgaccacag cccgttggtg tagccgagcg
     1321 cactgagcac cgcatgggtg tgaagccgca tcacgcggcc gttgccgtcg atgaaggggt
     1381 ggacccagcc gagccgctgg tgcgccgccg cgagcgccac caacgcggct tcgccgcgcc
     1441 gcacgccgcg gtagaacttg gcccaacgct gcagaaacgc cgcgaggctc gcatgcgtcg
     1501 gcgcgacgtg ttcgccgacc cgcacgtcgc gcaggcgaag ctggcccggc acgatgggtt
     1561 cgccttcggg cgtgaccagg tctgtcgccg gcaggcgcgc gaacagttcg cggtgcaggt
     1621 cttgaacggc gtccacgctg tacaagcgcg tggcgccggc gtcatcggca tagcgcgcct
     1681 cgatcgcagc ttcggcttcg atgtgggcca cggccagccg ttgccgtgcg gcgagattct
     1741 tgtcgtgcga gaagtctcgg cgcagcgctt gttcgatctc gtgcggccgc gtgtgctggc
     1801 cctcgatgcg gttcgtgtag tacgagttca tgccgcgcag cagcgtgcgc agttcgcggg
     1861 caagcggcaa accggccagt tgcgtggcgg tgcgcgccag ctcgtgcgcc tttgccagca
     1921 gcggttcttg cggcttggcg gcgggcatca acggctcgaa tcgatggggc aaatcttggc
     1981 cggtcattgc caagattatc aatcgttcaa ctcattgatg caattggctt tttcatcaca
     2041 tctgcgatat tatttgccaa cttcgttgcg aggtccggcg agttcaataa tgtggcggca
     2101 gctcatcgcg caagctgcgc ggtgcgccgc cctcgccggc cggcgcctgc tggcgcagcg
     2161 ccgccacctc acgcagcagc cagtcgatct gcgtctgctg ccgcgcgacc agcgcgttca
     2221 ggtgatcgac caggtcttcg gcgaagctcg ccttgatctc gaggtcggtc aggcgctgtt
     2281 cgagctgctc gttcgtcatc gggtaggtac tggcaggccg cctacgggtc gaacacggca
     2341 ccaacagact gtgagcaatt gatccccgaa ccgagcctcg gtctgagcct gaactgaagc
     2401 ggcgttggcc ctgcggaagt gcaacggttg tgcctccact atgtagggcc agaaagtagc
     2461 aacgtcacct tgtgcgagag ttccggcatg tgcaatgcga tcgtgatgat cgctgccgca
     2521 gcttgaaggg cctgcagcac tcgcttccca acctcgtgat cggtcaggaa cgccttgaac
     2581 tcgctgtcaa cagtttggtg gtagtacagc gactcgactt gccgcaacac cggtactcca
     2641 cctgtgattc gatagtcccg aaacgcctgc atcatcgcgt gcagctcgat cacagtgtgc
     2701 cgtcgaagtt caccactgat accctccgcg gccttgactt gctcaatggc ttcgacgagc
     2761 cgggttgtcg tattccgtca tggccgcctt agataagctg cgcttgtttg gtaggccgcc
     2821 cgtgagtcga acacggcacc aacagattat gagtctgctg ctctaaccat catgagctag
     2881 cggcccgaag ctgcgcattg taggtttgcg cagcgcacgc tacttcgtgc tcagcgcatt
     2941 gctgaccagc ttcgacgtga tgtcgacgat ctggatcatg cggtcgtagg ccatgcgcgt
     3001 cggcccgatc acgccgagcg tgccgacgat ctggccgtcg acctcgtacg gcgccgagac
     3061 caccgacagc tcctcgaacg gcacgaccat gctctcgccg ccgatgtaga tgcgcacgcc
     3121 ctcggcgcgg ctgctgacct cgagcagccg catcagctgg gtcttctgct cgaacacgtc
     3181 gaacagcttg cgcagcgaac ccatgtcgct cccgaagtcc tgcaccgcca gcaggttgcg
     3241 ctcgccgctg atcacgacct gctcctggct gtcggcatgc gcctcgctgc cggcctgcac
     3301 cgcggcctgc atcagcgcgg cgatctcgcc gcgcagcgcg tcgacctcgg tcttcagccg
     3361 ttcgcgcacc gcctcgatgg tcaggccgcg gtagtgcgcg gtcaggtagt tgctcgcctc
     3421 gatcagctgc gactgggtgt agtcctgcgc ggtgaagatc acgcggttct gcacgtcgcc
     3481 gtcgggcgcg accaggatca tcagcacgcg ccgctcgctc aggcgcatga actcgatgtg
     3541 gtggaacacg ctggccttgc gcggcgcggt gatcacgccg acgaagtgcg acaggttcga
     3601 cagcagctgc gccgcgttgg cgatcacgcg ctgcggctgg tccggcggca gctgcggtgc
     3661 cgcgaccgcg gcgaacggct gggccgtcag catcgtgtcg acgaacaggc gatagccgcg
     3721 cgccgtcggg atgcgcccgg cgctggtgtg ggggctggcg atcaggccga ggtcttcgag
     3781 gtcggccatc acgttgcgta tcgtggccgg cgaaagctcc agccccgaag cgcgcgacaa
     3841 ggtgcgcgag cccaccggct ggccgtctgc gatatagcgc tcgaccagcg ctttgagcag
     3901 tgttctga
//
LOCUS       DATMJB010000118         3950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_164032, whole genome shotgun sequence.
ACCESSION   DATMJB010000118 DATMJB010000000
VERSION     DATMJB010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164032"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VNU48_02655"
     CDS             <1..440
                     /locus_tag="VNU48_02655"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWI10205.1"
                     /translation="EPASVVGFWFAIEDAHRGNGCLGALVGQHRRGLKEVFRRQSDGR
                     LALEPQHPAPHWDMSPPGRPEGEYRNAQHEGTPMSALEWIEVRQGALVVFDGGLPHLS
                     EANRSAQSRHAYTLHAVDGRAHYPASNWIQRREGVDPPWRGFG"
     gene            539..2785
                     /locus_tag="VNU48_02660"
     CDS             539..2785
                     /locus_tag="VNU48_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWI10206.1"
                     /translation="MPKIARRAAPAPASVACRATLGLIGPLLALALPLARAQNAAPPG
                     TLQTVTVTAERRTENIKDVPSSVSIIAGEKLDVLNSSGEDLRALSGRVPSLNIESSFG
                     RAFPRFYLRGYGNTDFRLNASQPVSLVYDDVVQESPILKGFPAFDLERIEVLAGPQGT
                     LFGRNTPGGVVKFDSVQPSQSQEGYLSATVGRFGTVNLEGAFNLPLSGAWASRISLQT
                     QNRDDWVRNTAPVRQTDEMEGYNDNALRAQLLYKPGSDFSALFNLHGRDLKGSARLFR
                     ANIIKQGSNALVDDFDPYSISIDGKNEQKLQTYGGSARLKWALGSVNLYSITGYETVH
                     AFSRGDVDGGYGASFAPPSGPGLILFPLETADGIRGHGQLTQEFRVESATPGPLKWQA
                     GLYYFHEKYEVDTYDFNTLAGGVQDGFVPTSQKSDSYAVFGSVNYELTPALKLRAGLR
                     YTHDKKDLETQAAPGVIATNGLSQSRSDSKVSWDVSGTYALTPDVNLYARIATGYRGS
                     SIQPPSTFSALTSAAPETVLSYEGGAKADLFDKAARVSLSAYQYDVKDQQLTAVGGAS
                     NATQLVSAEKMTGRGVEATLDAYVTERLFVSVSGSYNHTQIKDPNLAVGVCAQCTVTD
                     PLNGSGNALIDGNPLPNAPRYVANLNLRYGIPTASGGEWYVYTDWSYRSKVNFFLYES
                     VEFTGPATTIGGLRLGYIWGNGKYEAAVFARNVTNQIRVNGGIDFNNLTGFINEPRFW
                     GAQFKAQF"
     gene            2959..3930
                     /locus_tag="VNU48_02665"
     CDS             2959..3930
                     /locus_tag="VNU48_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugE"
                     /protein_id="HWI10207.1"
                     /translation="MHFRHLIAACAASLLAVSGGAWAQAYPNKPVRLVVPFAPGGTTD
                     IVARVLAEKIGGPLGQTLIVENKAGGGGSIGATDTAKSAPDGYSLGVATVSTTASNPA
                     INPKLAYNPLTDFTPIINVAATPNVIAVHPSFPAHDYKGFLAELKKNPGKYSFGSSGT
                     GGIAHLQMELFKNLSGTFITHIPYRGSGPALNDAVAGQIPIIFDNLPSALPFIKDGRL
                     IAIVVAAPQRLAVLPNVPTFKEVGLEPVNRMAFYGIYGPKGLPKEVVDKVSSAVKKTL
                     EDPAVRKRIEDTGSLIVGNSPAEFAAQIAAEYEVYKKVVKLQKLTLE"
BASE COUNT          687 a         1381 c         1307 g          575 t
ORIGIN      
        1 ccgagcccgc ctcggtggtc ggcttctggt tcgcgatcga ggacgcgcac cgcggcaacg
       61 gctgcctggg cgcgctggtc gggcagcacc ggcgcggttt gaaggaggtg ttccggcgcc
      121 agtccgatgg ccgcctcgcg ctcgagccgc agcacccggc gccgcactgg gacatgagcc
      181 cgccgggccg ccccgagggc gaataccgga atgcgcagca cgaaggtact ccaatgagcg
      241 cgctcgaatg gatcgaggtg cgccagggcg cgctggtcgt gttcgacggc ggcctgccgc
      301 acctgtcgga ggcgaaccgt tccgcgcagt cgcgccacgc ctacacgctg cacgcggtcg
      361 acggtcgtgc gcactacccg gcgagcaact ggatccagcg ccgcgaaggc gtcgacccgc
      421 cgtggcgcgg cttcgggtaa atcctcgagc cggcgagcgc gagagcggtg ccgaatgggc
      481 gccgtgctgg cacacggctt gcacaggtcg ggcacacgac aaactcgccg gagggttcat
      541 gcccaagatc gctcgtcgcg ccgcgcctgc gcccgcttca gtcgcctgcc gcgcgacgct
      601 cggtctcatc ggcccgctgc tggcgctcgc gctgccgctg gcgcgggccc agaacgcggc
      661 gccgcccggc acgctgcaga ccgtcaccgt gaccgccgag cgccgcaccg agaacatcaa
      721 ggacgtgccg agctcggtct cgatcatcgc gggcgaaaag ctcgacgtcc tcaactcctc
      781 cggtgaagac ctgcgcgcgc tgtcgggccg ggtgccgagt ctgaacatcg aatcgtcgtt
      841 cggccgcgcg ttcccgcgct tctacctgcg cggctacggc aacaccgact tccgcctgaa
      901 cgcgtcgcag ccggtgtcgc tggtctacga cgacgtggtt caggagagcc cgatcctgaa
      961 gggctttccg gccttcgacc tcgaacgcat cgaggtgctg gccgggccgc agggcacgct
     1021 gttcgggcgc aacacacctg gcggcgtcgt caagttcgac tcggtccagc cctcgcaaag
     1081 tcaagagggc tacctcagcg cgacggtcgg ccgcttcggc accgtcaacc tcgaaggggc
     1141 gttcaacctg ccgctcagcg gcgcctgggc gtcgcgcatc tcgctgcaga cgcagaaccg
     1201 cgacgactgg gtcaggaaca ccgcgccggt gcgccagacc gacgagatgg agggctacaa
     1261 cgacaacgca ttgcgcgccc agcttctgta caagccgggc agcgacttca gcgcgttgtt
     1321 caacctgcat ggccgcgatc tgaagggcag cgcgcgcctg tttcgcgcca acatcatcaa
     1381 gcagggcagc aatgcactgg tcgacgactt cgacccgtac agcatctcga tcgacggcaa
     1441 gaacgaacag aagctgcaga cctacggtgg cagcgcgcgc ctgaagtggg cgctgggcag
     1501 cgtcaacctg tactcgatca ccggctatga aaccgttcac gccttcagcc gcggcgatgt
     1561 ggacggcggc tacggcgcga gtttcgcgcc gccgtcgggg cccgggctga tcttgttccc
     1621 gctcgaaacg gccgacggga ttcgcggcca cggccagctg acgcaggagt tccgggtcga
     1681 gtcggccacg ccggggccgc tcaaatggca ggccggcttg tactacttcc atgaaaagta
     1741 cgaggtcgac acctacgact tcaacacgct cgccggcggc gtgcaggacg gattcgtccc
     1801 cacgagccag aagagcgatt cctacgcggt gttcggctcg gtcaactacg aactcacgcc
     1861 ggcgctcaag ctgcgcgccg ggctgcgcta tacgcacgac aagaaggacc tcgaaacgca
     1921 agccgctccc ggtgtgatcg cgacgaacgg cctgtcccaa agccgcagcg attcgaaggt
     1981 cagctgggac gtctccggca cctatgcgct gacgcccgac gtgaacctgt acgcacgcat
     2041 cgcgaccggc taccgcggct cgagcatcca gccgccctcg acgttctcgg cactgacctc
     2101 ggcggcgccg gagacggtgc tgtcgtacga aggcggcgcc aaggccgacc tgttcgacaa
     2161 ggccgcgcgc gtgtcgctca gcgcgtacca gtacgacgtg aaggaccagc agctcaccgc
     2221 cgtcggtggg gcgagcaacg cgacgcagtt ggtcagcgcg gagaagatga ccggccgcgg
     2281 cgtcgaagcg acgctcgacg cctacgtgac cgagcgcctg ttcgtgtcgg tcagcgggag
     2341 ctacaaccat acgcagatca aggatccgaa cctggccgtc ggggtgtgcg cgcagtgcac
     2401 cgtgaccgac ccgctcaacg gcagcggcaa cgccctcatc gacggcaacc cgttgccgaa
     2461 cgcgccgcgc tacgtggcca acctgaacct gcgctatggc atcccgacgg cgagcggcgg
     2521 cgagtggtat gtctacaccg actggtcgta ccgcagcaag gtcaacttct tcctctacga
     2581 atcggtcgag ttcaccgggc cggccacgac gatcggcggg ctgcgcctgg gctatatctg
     2641 gggcaacggc aaatacgagg ccgcggtgtt cgcgcgcaac gtcacgaacc agatccgggt
     2701 gaacggcggc atcgacttca acaacctgac cggcttcatc aacgagccga ggttctgggg
     2761 cgcgcagttc aaggcgcagt tctagagccg cggggcggcc cgagcgcggc ggcatcgcgc
     2821 ggcggcacgc tccgcgcgat gcaccgggcc atccgggccg gcctcgccgg tggggcaggg
     2881 tgttcgctct gtcgcggcgc acggtcgcgg ccctagactt gcccgtcgtc tcgagcccgc
     2941 tcaccggaga gtccaccgat gcatttcaga catctgatcg ccgcctgcgc cgccagcctg
     3001 ctcgccgtct ccggtggcgc ctgggcccag gcctacccga acaagccggt ccggctggtg
     3061 gtgccgttcg cacccggcgg cacgaccgac atcgtcgcgc gcgtgctggc cgagaagatc
     3121 ggtgggccgc tgggccagac cctgatcgtc gagaacaagg ccggcggcgg cggctcgatc
     3181 ggtgccaccg acaccgccaa gtccgcgcct gacggctact cgctcggcgt ggccacggtg
     3241 tcgacgaccg cgtcgaaccc ggccatcaac ccgaagctgg cgtacaaccc gctcaccgat
     3301 ttcacgccga tcatcaacgt cgccgcgacg ccgaacgtga tcgcggtgca cccgagcttt
     3361 ccggcgcacg actacaaggg cttcctcgcc gagctcaaga agaaccccgg caagtacagc
     3421 ttcggcagtt cgggcaccgg cggcatcgcg cacctgcaga tggagctgtt caagaacctg
     3481 tcgggcacct tcatcacgca cattccgtac cgcggctcgg gcccggcact gaacgacgcg
     3541 gtcgccgggc agatcccgat catcttcgac aacctgccct cggcgctgcc cttcatcaag
     3601 gacggccgcc tgatcgcgat cgtcgtcgcg gcgccgcagc gcctggcggt gctgcccaac
     3661 gtgccgacct tcaaggaagt gggcctcgag ccggtcaacc gcatggcctt ctacggcatc
     3721 tacgggccca agggcctgcc gaaggaagtg gtcgacaagg tcagcagcgc ggtcaagaag
     3781 acgctcgagg acccggccgt caggaagcgc atcgaagaca ccggctcgct gatcgtgggc
     3841 aactcgccgg ccgaattcgc ggcccagatc gcggccgagt acgaggtgta caagaaggtc
     3901 gtgaagctgc agaagctgac gctggagtga gcgtctgacc accccggccc
//
LOCUS       DATMJB010000119         3176 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_101928, whole genome shotgun sequence.
ACCESSION   DATMJB010000119 DATMJB010000000
VERSION     DATMJB010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101928"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="VNU48_02670"
     CDS             <1..1316
                     /locus_tag="VNU48_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWI10208.1"
                     /translation="FEPKTRATSQQELITTLLAHTSLESSAPINLTMIGADGRPTQKS
                     LRQMLAEWLAFRAETVQRRTQHRLARVLARIHVLEGRQLVLLNIDAVIRIIREADEPK
                     AALIARFALSEIQADDILDIRLRQLARLEAIRIEQELKELRVEQGKLEDILNSPASLK
                     RLLIKEIELDAKTHGDARRTLIQAEKRSVAEIRIVDEPVTVVVSLKGWVRGLKGHEIE
                     TASLAFKAGDALYGTFACRSVDALLVFGSNGRVYSTPVAQLPGGRGDGQPITSLIELE
                     SGTQPAHYFAGHLQQTLLLAGTGGFGLLARVGDLLARQRGGKSFLSLEADEKPLPPSL
                     TDGLAQVACLSLAGRLLVFPLDELKLQANGGRGLTLIDLDAKDALVSVAAFGATLQML
                     GSGRGGKPKDELLRAAALTAYAGKRARKGRVQDVMPKAMRVLRAD"
     gene            1432..1956
                     /locus_tag="VNU48_02675"
     CDS             1432..1956
                     /locus_tag="VNU48_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWI10209.1"
                     /translation="MTTTTITDPIQARTTLWSLIKDIRFAMFTTRHVGGQLQARPMTT
                     QNSSLDEDSNLWFFMSRRGDPVAELSAEPGVNVSYSDPDKDRYVSVSGTASVIENTAK
                     KQQLWTKLAEAWFPGGIGDPDLALVRVRIVDASYWDVKESKVVQFYRMAKAAITGKRP
                     TELGEHAEIHMNVN"
     gene            1970..2851
                     /locus_tag="VNU48_02680"
     CDS             1970..2851
                     /locus_tag="VNU48_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWI10210.1"
                     /translation="MSVFFDISHSTHYRYHQPVRLGEHRVLFRPRSSHDLRVLATDLQ
                     VTPEPLDIRLIQDVYSNSVALVQPQSPATELKLECTFSVEHTGTRALDLPLKPSASAY
                     PFAYSDEDRIALQHYLLPYYDDPSGELEAWARGFVHDDGSTGTRELLVEMTQTIRNSM
                     RYVARFDEGVQTPYDTLHLQSGTCRDFATLMIEAVRRLGYAARFVSGYLYTPWLDDGA
                     PEHVGAGATHAWVQVYLPGAGWIPFDPTNNLIGGTDLIRAGVARHAGLASPVSGSWVG
                     RPGDFAGMFVDVQVRRR"
     gene            complement(2858..>3176)
                     /locus_tag="VNU48_02685"
     CDS             complement(2858..>3176)
                     /locus_tag="VNU48_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HWI10211.1"
                     /translation="VWRSAAGQMITGGVALLLALCGVAAALWQHFQAAASASCNLTLA
                     DKIVSGWLGLDSLLPDVFSARASCADAAVKLFGVPYDFWSLALFVLISIALLRVLRRP
                     ARR"
BASE COUNT          535 a         1114 c         1084 g          443 t
ORIGIN      
        1 tgttcgagcc gaagacgcgc gcgacctccc aacaggaact gatcaccacc ttgctcgcgc
       61 acacctcgct cgagagctcg gcgccgatca acctgacgat gatcggtgcc gacggccggc
      121 cgacgcagaa aagcctgcga cagatgctgg ccgagtggct cgcgttccgc gccgagacgg
      181 tgcagcgccg cacgcagcac cggctcgcgc gggtgctcgc gcgcatccat gtgctcgaag
      241 ggcggcagct ggtgctgctc aacatcgacg cggtgatccg catcatccgc gaggccgacg
      301 agccgaaggc cgcgctgatc gcgcgcttcg cgctgagcga gatccaggcc gacgacatcc
      361 tcgacatccg cctgcgccag ctggcacggc tcgaggcgat ccggatcgag caggagctga
      421 aggagttgcg cgtcgagcag ggcaagctcg aagacatcct gaacagcccg gcctcgctga
      481 agcggctgct gatcaaggag atcgagctcg atgcgaagac gcacggcgac gcgcgccgca
      541 ccctgatcca ggccgagaag cgctcggtcg ccgagatcag gatcgtcgac gagccggtca
      601 cggtggtcgt cagcctgaag ggctgggtgc gcggcctgaa gggccacgag atcgagaccg
      661 cgagcctggc gttcaaggcc ggcgacgcgc tgtacggcac cttcgcgtgc cgcagcgtcg
      721 acgcactgct ggtgttcggc agcaacggcc gcgtctattc gacgccggtc gcgcagctgc
      781 ccggcgggcg cggcgacggc cagccgatca cctcgctgat cgagctcgaa tcgggcacgc
      841 agccggcgca ctatttcgcc ggccatctgc aacagaccct gctgctcgcc ggcaccggcg
      901 gcttcggctt gctggcgcgc gtcggcgacc tgctggcgcg ccagcgcggc ggcaagagct
      961 tcctcagcct cgaggccgac gagaagccgc tgccgccgag cctgaccgat gggctggccc
     1021 aggtcgcgtg cctgtcgctg gccggccggc tgctcgtgtt cccgctcgac gagttgaagc
     1081 tgcaggccaa cggcggccgc ggcctgacct tgatcgacct ggacgcgaag gacgcgctcg
     1141 tcagcgtcgc ggccttcggc gcgacgctgc agatgctggg cagcggccgc ggcggcaagc
     1201 cgaaggatga actgctgcga gctgccgcgc tgaccgcata cgccggcaag cgcgcccgca
     1261 agggccgcgt gcaggacgtg atgccgaagg cgatgcgggt gctgcgcgcc gactgagcgg
     1321 ggcaaaccgg acacgtccta cacgccccag gcgtcgcgcc tgatcgcgcc actgcgcggc
     1381 gcttgatgca atgggcgctc cggtttgccg catccggcga aaggagcacg catgaccacc
     1441 acgacgatca ccgatccgat ccaggcccgc acgacgctgt ggagcctgat caaggacatc
     1501 cgcttcgcga tgttcaccac ccgccacgtc ggcggccagc tgcaggcgcg gccgatgacg
     1561 acgcagaact ccagcctcga cgaggattcg aacctgtggt tcttcatgtc gcgccgcggc
     1621 gacccggtgg ccgagctgag tgccgagccc ggcgtcaacg tgagctactc cgaccccgac
     1681 aaggaccgct atgtctcggt gtccggcacc gccagcgtga tcgagaacac ggcgaagaag
     1741 cagcagctgt ggacgaagct cgccgaagcc tggttccctg gcggcatcgg cgaccccgac
     1801 ctcgcgctgg tgcgcgtgcg gatcgtcgac gcgagctatt gggacgtgaa ggagagcaag
     1861 gtcgttcagt tctaccggat ggcgaaggca gcgatcaccg gcaagcggcc gaccgagctg
     1921 ggcgagcacg ccgagatcca tatgaacgtg aactgaaagc cgcgcgcgcg tgtccgtctt
     1981 cttcgacatc agccacagca cgcactaccg ctatcaccag ccggtgcgcc tgggcgaaca
     2041 ccgcgtgctg ttccgcccgc gcagcagtca cgacctgcgc gtgctcgcga ccgatctgca
     2101 ggtgacgccg gagccgctcg acatccggct gatccaggac gtgtactcga actcggtcgc
     2161 gctggtgcag ccgcaaagcc cggcgaccga gctgaagctg gagtgcacgt tctcggtcga
     2221 gcacaccggc acgcgcgcgc tcgacctgcc gctgaagccg agcgcgtcgg cctatccgtt
     2281 cgcctacagc gacgaggacc gcatcgcgct gcagcactac ctgctgccgt actacgacga
     2341 cccgagcggc gagctcgagg cctgggctcg gggcttcgtc cacgacgacg gcagcaccgg
     2401 cacgcgcgaa ttgctggtcg agatgacgca gacgatccgc aattcgatgc gctacgtcgc
     2461 gcgcttcgac gagggcgtgc agacgccgta cgacacgctg cacctgcagt ccggcacctg
     2521 ccgcgatttc gcgaccttga tgatcgaggc ggtgcgtcgg ctcggctacg cggcgcgatt
     2581 cgtttcgggc tacctctaca cgccgtggct cgatgatggc gcccccgagc acgtcggtgc
     2641 cggggcgacc cacgcgtggg tgcaggtcta tctgccgggg gccggctgga ttccgttcga
     2701 cccgaccaac aacctgatcg gcggcaccga cctcatccgc gccggcgtcg cgcgccatgc
     2761 gggcctggcg tcgccggtca gcggctcgtg ggtcggccgg ccgggcgact tcgccggcat
     2821 gttcgtcgat gtgcaggtgc ggcggcgcta gccaatttca gcgacgcgcc ggtcgacgca
     2881 gcacgcgcag cagcgcgatc gagatcagca cgaacagcgc caggctccag aagtcgtagg
     2941 gcacgccgaa cagcttgacc gccgcatcgg cgcagctcgc ccgcgccgag aacacgtcgg
     3001 gcagcaggct gtccagcccg agccagccgc tgacgatctt gtcggccagg gtcagattgc
     3061 acgacgccga cgccgcggcc tggaagtgct gccacagtgc cgccgcgacg ccgcacagcg
     3121 cgagcagcag cgccacgccg ccggtgatca tctgcccggc ggcactgcgc catacc
//
LOCUS       DATMJB010000120         2912 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_279809, whole genome shotgun sequence.
ACCESSION   DATMJB010000120 DATMJB010000000
VERSION     DATMJB010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279809"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VNU48_02690"
     CDS             <1..489
                     /locus_tag="VNU48_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10212.1"
                     /translation="ARQQEINMSDTHDVMMNEIRYAERLCLRTARLYRRAQTIGVFVT
                     IVGGSAAMSALSSSAPKWLSVAGFISLTLFGAAMIAIRPAEKATANEADAKRYGQLRT
                     AANTMDDLTLRAAIDKAREGDGPEIEPLRDVAYNDVVVEVGRPDVAIPLTTKQKFLAA
                     LA"
     gene            696..1598
                     /gene="cysM"
                     /locus_tag="VNU48_02695"
     CDS             696..1598
                     /gene="cysM"
                     /locus_tag="VNU48_02695"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HWI10213.1"
                     /translation="MKYASVEDVIGNTPLVRLARIPGLDNEQRGNIILGKLEGNNPAG
                     SVKDRPAISMIKRAEERGEIKPGDTLIEATSGNTGIALAMAAAIRGYRMLLIMPEDLS
                     IERAQTMKAFGAELILTPRSGGMEYARDLAEQMHKDGKGRVLDQFANADNPRVHYEAT
                     GPEIWRDTKGRVTHFVSAMGTTGTITGVSRYLKEQSPAVRVIGAQPSEGSRIPGIRKW
                     PEAYLPKIYDPTFIDEMVYVGQADAEEMARRLAREEGLFAGISAAGACWVAMQIAKQV
                     ENATIVFIVCDRGDRYLSTGVFPA"
     gene            1604..2152
                     /locus_tag="VNU48_02700"
     CDS             1604..2152
                     /locus_tag="VNU48_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWI10214.1"
                     /translation="MAHQFNYCPNCGTPLELLARLEDGGEKSRLRCPACAFTHWNNPT
                     PVLAAVIEMADRDGQVLLARNAAWPGRMFALITGFMEAGETPEEGVAREVLEETSLKV
                     DALKLIGVYDFQRMNQIIIAYHAIARGEITLSPELAEYRLYRPEDIKCWRAGTGYAVA
                     DWLTARGITPQWVDLPPRPAAT"
     gene            2177..2404
                     /locus_tag="VNU48_02705"
     CDS             2177..2404
                     /locus_tag="VNU48_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HWI10215.1"
                     /translation="MDAQKELDTRGLNCPLPILKAKKALADMRSGEVLKVVSTDPGST
                     RDFQAFARQTGNELVEQSSSNDEFIHFLRRR"
     gene            complement(2420..>2912)
                     /locus_tag="VNU48_02710"
     CDS             complement(2420..>2912)
                     /locus_tag="VNU48_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWI10216.1"
                     /translation="FAVLLADDLMVGSTPILKQMVEQYEEWRVSILAVQEVPAEHTRR
                     YGIVDGTLINDRLMDVTGIVEKPAPEDAPSRLGVAGRYILTPGVFHEIANQARGVGGE
                     IQLTDGIAGLLRREKVFAYRYEGRRYDCGSKEGFLEANVELALAHPTLGPGFRSFLQG
                     LGI"
BASE COUNT          524 a         1000 c          960 g          428 t
ORIGIN      
        1 gcccgccagc aggagataaa catgagcgac acccacgacg tgatgatgaa cgagatccgc
       61 tatgcggagc gcctttgcct gcgcacggcg cgcctttaca ggcgtgcgca aaccatcggt
      121 gtgttcgtga cgatcgtcgg cggcagcgcg gcgatgtcag cgctgtcaag cagcgcgccc
      181 aaatggctca gcgtggcagg gttcatctcg cttactcttt tcggtgcggc gatgattgcg
      241 atccggccgg ccgagaaggc gaccgccaac gaggctgacg cgaagcgcta cgggcagctg
      301 cgcaccgcgg ccaacaccat ggatgacctt acgctgcgcg ctgcgataga caaagcgcgc
      361 gagggtgacg ggccggagat cgaacccctg cgcgacgttg cctacaacga tgtggtcgtc
      421 gaagtggggc ggcccgacgt cgccatccca ttgaccacga agcagaagtt cctcgcggcc
      481 ctggcctgat tgcgttcatc gaagttgggt ttccgtttcg ctacactggt ccggcatctc
      541 gacgacgttc gggctctgcc gacgccggcg cgaggccgcg ccgcaagaga gatgcgaccc
      601 gaggcaccac aagcctgccc agcgctccgt ccgatggcct gcgagaatcg cggcgaggag
      661 agtccatgag cgccaaatcg tcacgcagcc cgccgatcaa atacgccagc gtcgaagacg
      721 tcatcggcaa cacgccgctg gtgcggctgg cacggattcc cggcctggac aacgaacagc
      781 gcggcaacat catcctcggc aagctcgagg gcaacaaccc ggccggctcg gtgaaggacc
      841 ggcccgcgat cagcatgatc aagcgtgccg aggagcgcgg cgagatcaag cccggcgaca
      901 ccctgatcga ggcgacctcg ggcaacaccg gcatcgcgct ggcgatggcg gcggccatcc
      961 gcggctaccg catgctgctg atcatgccgg aggacctgag catcgagcgc gcgcagacga
     1021 tgaaggcctt cggcgccgaa ctgatcctga cgccgcgttc gggcggcatg gagtacgccc
     1081 gcgatctcgc cgagcagatg cacaaggacg gcaagggccg cgtgctcgac cagttcgcga
     1141 acgccgacaa cccgcgtgtg cactacgagg cgaccgggcc cgagatctgg cgcgacacga
     1201 aggggcgtgt cacgcacttc gtcagcgcga tgggcacgac cggcacgatc accggcgtct
     1261 cgcgctacct gaaggagcag agccctgccg tgcgcgtgat cggcgcgcag cccagcgaag
     1321 gctcgcgcat cccgggcatc cgcaagtggc ccgaggccta cctgccgaag atctacgacc
     1381 cgaccttcat cgacgagatg gtctatgtcg gccaggccga tgccgaggag atggcgcgcc
     1441 gcctggcgcg cgaggaaggc ctgttcgccg gcatctcggc ggcgggcgcc tgctgggtcg
     1501 caatgcagat cgccaagcag gtcgagaacg cgacgatcgt gttcatcgtc tgcgaccgcg
     1561 gcgatcgcta cctgtccacc ggcgtgttcc cggcctgatc gcgatggcac accagttcaa
     1621 ctactgtccg aactgcggca cgccgctgga gctgctcgcg cggctcgaag acggcggcga
     1681 gaagtcgcgc ctgcgctgcc cggcctgcgc gttcacgcac tggaacaacc cgaccccggt
     1741 gctcgccgcg gtgatcgaga tggccgatcg cgacggccag gtcctgctgg cgcgcaacgc
     1801 cgcctggccg ggccggatgt tcgcgctgat caccggtttc atggaagccg gtgagacgcc
     1861 cgaggagggc gtcgcgcgcg aggtgctcga ggagacgagc ctcaaggtcg acgcgctgaa
     1921 gctgatcggc gtctacgact tccagcgcat gaaccagatc atcatcgcgt accacgcgat
     1981 cgcgcgcggc gagatcacgc tgtcgcccga actggccgag taccggctct accggcccga
     2041 agacatcaag tgctggcgcg ccggcaccgg ctacgcggtg gccgactggc tgaccgcccg
     2101 cggcatcacg ccgcagtggg tcgacctgcc gccgcgcccg gccgcgacct agaatcgcgg
     2161 cacacgatcg gaaaccatgg acgcccagaa agaactcgac acccgcgggt tgaactgccc
     2221 gctgcccatc ctgaaggcga agaaggcgct ggccgacatg cgcagcggcg aggtgttgaa
     2281 ggtcgtctcg accgacccgg gttcgacccg cgactttcag gctttcgcgc gccagacggg
     2341 caacgaactc gtcgagcagt cgagcagcaa cgacgagttc atccacttcc tgcggcgccg
     2401 ttaggcgccg gcgcgcacct cagatcccga ggccctgcag gaagctgcga aaccccgggc
     2461 cgagcgtcgg gtgcgccagc gccagttcga cattcgcctc cagaaagcct tccttgctgc
     2521 cgcagtcgta gcgcctgcct tcgtagcggt aggcgaagac cttctcgcgc cgcagcagcc
     2581 cggcgatgcc gtcggtgagc tggatctcgc cgcccacgcc gcgcgcctgg ttggcgattt
     2641 cgtgaaatac gcccggcgtc aggatgtagc ggccggccac gcccaggcgt gacggcgcgt
     2701 cctccggcgc aggcttctcg acgatgcctg tcacgtccat cagccgatcg ttgatcagcg
     2761 tgccgtcgac gatgccatag cgccgcgtgt gctcggccgg cacctcctgc accgccagga
     2821 tcgagacgcg ccactcctcg tactgctcga ccatctgttt caggatcggc gtgctgccca
     2881 ccatcaggtc gtctgccagc aacaccgcga at
//
LOCUS       DATMJB010000121         5252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_457345, whole genome shotgun sequence.
ACCESSION   DATMJB010000121 DATMJB010000000
VERSION     DATMJB010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457345"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1055
                     /locus_tag="VNU48_02715"
     CDS             147..1055
                     /locus_tag="VNU48_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWI10217.1"
                     /translation="MPDSVDAVVTLLATENYVCDRRLATALFLSLKLARPLFLEGEPG
                     VGKTELAKTLAAALSTALLRVQCYEGLDIAQTAYEWNVARQMVEIRLAEAAHAIHGEG
                     DRERLAHTLYSRSMLIERPLLQALTQSRSPVLLIDELDRADEPFDAFLLEVLAENQIT
                     IPEFGTVKAAAKPITIITSNRTREIHDALKRRCLYHWVDFPTVERELEIVRLKAPGAA
                     DRLTVQVVEFVQRMRKLDLFKAPGVAETIDWTNALVALNAIRLDPATVHDTLGVLLKY
                     QDDIVKLQSGDTAKLLDELKARALID"
     gene            1064..2254
                     /locus_tag="VNU48_02720"
     CDS             1064..2254
                     /locus_tag="VNU48_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWI10218.1"
                     /translation="MIAENIVHFARVLRTAGMPIGPDRVLAAMAAVEHVGLNRRDDVH
                     AALGAVMLDRHEQQTLFDAAFDAFWRDPKLLEQMMYLLLPKISGRGDKPAPPRANRLA
                     DALAAPKPKEPPNPANATAQEELTFDTTFTFSDRERLQQADFESMTAAEFELAKKLAE
                     QVPLPVQPVRRRRHESAANRQRGGTLDLRQTMQRMARQPHTLLPAFTRARREWPTLVV
                     LLDISGSMERYARLLLHYVHGLTRRYLKVHTLVFGTRLTNITRSLRHRDPDVALQLAD
                     AQVQDWKGGTRIASCLDEFNRVWARRLLGSNAAVLLMTDGLDRDEHGDLARAAAQLRR
                     MAHRIVWLNPLLRFDGFEPRAAGVRALLPHVDRFLPVHNLASLADLGKALRAPSVAPS
                     LLLA"
     gene            2261..2833
                     /locus_tag="VNU48_02725"
     CDS             2261..2833
                     /locus_tag="VNU48_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HWI10219.1"
                     /translation="MELQGERTIPASVDTTWSALNDPQVLKSCIAGCESLERSGPDAM
                     TAVVALKVGPVSARFKGNLSMSNVQAPNSYTINFDGQGGVAGFGKGAADVALTAQGAQ
                     QTLLKYSARATVGGKMAQIGSRLIDATAAKITEDFFKAFEAHLQAGAAPAEGAVVVPP
                     APAESDGSMARLWWLIGAVVVLAIVYFAMR"
     gene            2846..3847
                     /locus_tag="VNU48_02730"
     CDS             2846..3847
                     /locus_tag="VNU48_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HWI10220.1"
                     /translation="MDSLDLQVLSQARDWLAQGHKVWLVTVIETWGSAPRPPGALLCL
                     RDDGQVAGSVSGGCVEDDLIDRLRHGERVATPSLIAYGVTKEEAARFGLPCGGNLRLV
                     QEPLQDAAWIDEVLARTARHELVARRLDLATGAVVVEAASRGDVFAFDGQQLVALFGP
                     RWRLLIIGAGQLSRAVAQMALMLDFEVICCDPREEYNLSWDIPGTTFSKAMPDDLVLE
                     LQLDPHSAVVAVTHDPKLDDLVLLEALKSPAFYIGALGSRGNTAKRRERLELFDLSAA
                     EIDRLHGPIGLDIGSKTPAEIAVSIVAEIVAVKNGVLLKQKKDGSAVPAPRPATSAG"
     gene            complement(3888..5222)
                     /locus_tag="VNU48_02735"
     CDS             complement(3888..5222)
                     /locus_tag="VNU48_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HWI10221.1"
                     /translation="MLLYAVTAHVLQQRRHPTAAIAWVLFIVLAPYVAMPAFLAFGSR
                     KQVRPRNRPLLAPPATDAEGPWAIQTALALGQPEPAAYTGLRIHADGQAALAALWQTI
                     AAAEHSIDLCTFIIGRDAVGYALIERLSAKARAGVKVRVMLDGLGSLMRSRPDLRPLR
                     AAGIEFLLFVPPLHSPLKGRTNLRDHRKMLIADASRATQHLWCGGRNLAGEYFEGRPG
                     QPAWHDLSFDLGGAFVQQASALFAHDWAFAGGAAGDAGPVAPPAGVSDATGAQLIASG
                     PDQADDTVYSLLVTAAYRARRRLLLITPYFVPDAALLTALCLAARRGVQVDLLLPAHS
                     NHRLSDVARRRALRTLGAAGGRIWTVPHMLHAKLAVIDDTLALAGSANLDSRSLFLNY
                     ELMVAFHDRDAIGRFAAWFEHEQAAAGRFTPRRAGLLTDIAEGLVLWLGFQL"
BASE COUNT          800 a         1848 c         1897 g          707 t
ORIGIN      
        1 gggcaaggcg ccagtgccga aggtcggcta cctgatcgcg ttcgacctga tcggcctggc
       61 gctgctggtg ggcgccatct acagcatggg agaattgaaa tgatccgcgc cgtccccgga
      121 acccccgcat gacgacgccg ctcgtgctgc ccgactcggt cgacgccgtc gtcacgctgc
      181 tggccaccga aaactacgtc tgcgaccggc gcctggcgac cgcgctgttc ctgagcctga
      241 aactggcgcg tccgctgttc ctcgagggcg agcccggggt gggcaagacc gagctggcga
      301 agacgctcgc cgcggcgctg tccaccgcgc tgctgcgcgt gcagtgctac gagggcctgg
      361 acatcgcgca gaccgcgtac gagtggaacg tcgcgcgcca gatggtcgag atccgcctgg
      421 ccgaggcggc gcacgcgatc cacggcgagg gcgaccgcga acggctggcg cacacgctgt
      481 actcgcgcag catgctgatc gagcgcccgc tgctgcaggc gctgacgcaa tcgcgctcgc
      541 cggtgctgct gatcgacgag ctcgaccgcg ccgacgaacc cttcgatgcg ttcctgctcg
      601 aggtgctggc agagaaccag atcacgatcc ccgagttcgg caccgtgaag gccgcagcaa
      661 agccgatcac catcatcacc agcaaccgca cgcgcgagat ccacgatgcg ctgaagcgcc
      721 gctgcctgta ccactgggtc gacttcccga cggtcgagcg cgagctcgag atcgtgcggc
      781 tgaaggcgcc gggcgcggcc gaccgcctga cggtccaggt cgtcgagttc gtgcagcgca
      841 tgcgcaagct cgacctgttc aaggcgccgg gcgtggccga gacgatcgac tggaccaacg
      901 cgctggtggc gctgaacgcg atccgcctcg acccggcgac ggtgcacgac acgctcggcg
      961 tgctgctgaa gtaccaggac gacatcgtca agctgcagag cggcgacacc gcgaagctgc
     1021 tcgacgagct gaaggcgcgc gcgctgatcg actgacccgc atcgtgatcg ccgaaaacat
     1081 tgttcatttc gcgcgcgtgc tgcgcacggc cggcatgccg atcgggccgg accgcgtgct
     1141 cgcggcgatg gccgcggtgg aacacgtcgg gctgaaccgc cgcgacgacg tgcacgccgc
     1201 actcggcgcg gtgatgctcg accgccacga gcagcagacg ctgttcgacg ccgcgttcga
     1261 cgcgttctgg cgcgacccga agctgctcga gcagatgatg tacctgctgc tgcccaagat
     1321 cagcgggcgc ggcgacaagc cggcgccccc gcgcgcgaac cggctcgccg atgcgctcgc
     1381 cgcaccgaag ccgaaggagc cgccgaaccc ggccaacgcc accgcgcagg aagagctcac
     1441 gttcgacacg accttcacgt tctcggaccg cgaacggctg cagcaggccg acttcgagag
     1501 catgacggcg gccgagttcg agctcgccaa gaagctcgcc gagcaggtgc cgctgccggt
     1561 gcagccggtg cgccggcgcc gccacgagag cgccgcgaat cgccagcgcg gcggcacgct
     1621 cgacctgcgc cagacgatgc agcgcatggc gcgccagccg cacaccttgc tgccggcgtt
     1681 cacgcgcgcg cggcgcgagt ggccgacgct ggtcgtgctg ctcgacatct ccggctcgat
     1741 ggagcgctac gcgcggctgc tgctgcacta cgtgcacggg ctgacgcgcc gctacctgaa
     1801 agtccacacg ctggtgttcg gcacgcggct gaccaacatc acgcgcagcc tgcggcatcg
     1861 cgacccggac gtcgcgctgc agctcgccga tgcgcaggtg caggactgga agggcggcac
     1921 gcgcatcgca agctgcctgg acgagttcaa ccgcgtctgg gcgcgccgcc tgctcggctc
     1981 gaacgccgcc gtgctgctga tgaccgacgg gctggaccgc gacgagcacg gcgacctggc
     2041 ccgtgccgcc gcgcagctgc gccgcatggc gcaccgcatc gtctggctca atccgctgct
     2101 gcgcttcgac ggtttcgagc cgcgcgcggc cggcgtgcgc gcgctgctgc cgcatgtcga
     2161 tcgcttcctg ccggtccaca atctggccag cctcgccgat ctcggcaagg ccttgcgcgc
     2221 gccgtcggtc gccccttcct tacttctggc ataagcactc atggaactgc aaggcgaacg
     2281 caccatcccg gcctcggtcg acacgacctg gtccgcgctc aacgatccgc aggtgctgaa
     2341 gtcgtgcatc gccggctgcg agtcgctcga gcgctcgggt cccgacgcga tgaccgcggt
     2401 ggtcgcgctg aaggtcggcc cggtcagcgc gcgcttcaag ggcaacctgt cgatgagcaa
     2461 cgtgcaggcg ccgaacagct acacgatcaa cttcgacggc cagggcggcg tcgccggctt
     2521 cggcaagggc gcggccgacg tggcgctgac cgcgcagggc gcgcagcaga ccctgctgaa
     2581 gtacagcgcg cgcgccaccg tcggcggcaa gatggcgcag atcggctcgc gcctgatcga
     2641 cgcgaccgcg gccaagatca ccgaggactt cttcaaggcc ttcgaggccc atctgcaggc
     2701 cggcgcggcg ccggcagagg gcgcagtggt ggtgccgccg gcgccggccg agtcggatgg
     2761 cagtatggcc aggctctggt ggctgatcgg cgccgtggtg gtgctggcga tcgtctattt
     2821 cgcgatgcgt tgaaagaggc aagagatgga cagtctcgat ctgcaggtgc tgagccaggc
     2881 gcgcgactgg ctcgcgcagg gccacaaggt ctggctcgtg acggtgatcg aaacctgggg
     2941 ctcggcgccg cgcccgcccg gcgcgctgct gtgcctgcgc gacgacggtc aggtcgccgg
     3001 ctcggtgtcg ggcggttgcg tcgaggacga cctgatcgat cggctgcgcc acggcgagcg
     3061 cgtcgccacg ccctcgctga tcgcctacgg cgtgaccaag gaagaggcgg cgcgcttcgg
     3121 cctgccgtgc ggcggcaacc tgcggctggt gcaggagccg ctgcaagacg cggcctggat
     3181 cgacgaggtg ctggcccgca ccgcgcgcca tgaactggtc gcgcgccggc tggatctggc
     3241 caccggcgcg gtcgtcgtcg aggcggcgtc gcgcggcgac gtgttcgcgt tcgacgggca
     3301 gcaactggtc gcgctgttcg gcccgcgctg gcggctgctg atcatcggtg ccggccagct
     3361 ctcgcgcgcg gtcgcgcaga tggcgctgat gctcgacttc gaggtgatct gctgcgaccc
     3421 gcgcgaggaa tacaacctga gctgggacat ccccggcacg accttcagca aggccatgcc
     3481 cgacgacctc gtgctcgaac tgcagctcga cccgcacagc gccgtcgtcg cggtcacgca
     3541 cgacccgaag ctcgacgacc tggtgctgct cgaagcgctg aagtcgccgg ccttctacat
     3601 cggcgcgctc ggctcgcgcg gcaacaccgc gaagcggcgt gagcgcctcg agctgttcga
     3661 cctgagcgcg gccgagatcg accggctgca cggcccgatc gggctcgaca tcggcagcaa
     3721 gacgccggcc gagatcgcgg tgtccatcgt cgccgagatc gtcgcggtga agaacggcgt
     3781 gctgctgaag cagaagaagg acggctcggc cgtgccggcg ccgcggccgg cgacctcggc
     3841 cggctgacag cgcagcggcc gacgcgcgcc gcgtcggccg cgccgcgtca gagctggaat
     3901 ccgagccaca gcaccaggcc ttcggcgatg tcggtcagca gcccggcgcg ccgtggcgtg
     3961 aagcgcccgg ccgccgcctg ctcgtgctcg aaccaggcgg cgaaacggcc gatcgcgtcg
     4021 cggtcgtgga acgccaccat cagctcgtag ttcaggaaca ggctgcggct gtcgaggttg
     4081 gccgagccgg ccagcgcgag cgtgtcgtcg atcaccgcga gcttggcgtg cagcatgtgc
     4141 ggcaccgtcc agatgcggcc accggccgcg ccgagcgtgc gcagcgcgcg gcgccgtgcc
     4201 acgtccgaca ggcggtggtt cgagtgcgcc ggcagcagca ggtcgacctg cacgccgcgc
     4261 cgcgccgcca ggcacagcgc ggtcagcagc gccgcgtcgg gcacgaagta gggcgtgatc
     4321 agcagcaggc ggcggcgtgc gcggtaggcg gcagtgacga gcagcgagta gaccgtgtcg
     4381 tcggcctggt cgggccccga ggcgatcagc tgcgcgccgg tggcgtcgga cacgccggcc
     4441 ggcggcgcca cgggcccggc gtcgcccgcg gcaccgccgg cgaacgccca gtcgtgcgcg
     4501 aacagcgcgc tggcctgctg cacgaacgcg ccgcccaggt cgaagctcag gtcgtgccac
     4561 gcgggctggc cgggccggcc ctcgaagtac tcgccggcca ggttgcgacc gccgcaccac
     4621 aggtgctgcg tcgcgcgcga cgcgtcggcg atcagcatct tgcggtgatc gcgcaggttg
     4681 gtgcggccct tcagcggcga gtgcagcggc ggcacgaaca gcaggaactc gatgccggcc
     4741 gcacgcagcg ggcgcaggtc gggccggctg cgcatcaggc tgcccaggcc gtcgagcatc
     4801 acgcgcacct tcacgccggc gcgcgccttc gcgctcaggc gttcgatcag cgcgtagccg
     4861 accgcgtcgc ggccgatgat gaaggtgcac aggtcgatcg agtgctcggc cgcggcgatc
     4921 gtctgccaca gcgcggcgag cgcggcctgg ccgtcggcgt ggatgcgcag ccccgtgtac
     4981 gcggccggct cgggctggcc gagcgcgagc gcggtctgga tcgcccacgg cccttcggca
     5041 tcggtggccg gcggcgcgag cagcggccgg ttgcgcgggc gcacctgctt gcgcgagccg
     5101 aaggccagga acgccggcat cgcgacgtag ggcgcgagca cgatgaacag cacccaggcg
     5161 atcgccgcgg tcgggtggcg gcgctgctgc agcacgtgcg cggtgacggc atagagcagc
     5221 accgcgacga tggtgacgaa accatgaacg gt
//
LOCUS       DATMJB010000122         5361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_457209, whole genome shotgun sequence.
ACCESSION   DATMJB010000122 DATMJB010000000
VERSION     DATMJB010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457209"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VNU48_02740"
     CDS             <1..205
                     /locus_tag="VNU48_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI10222.1"
                     /translation="GKAALDLVHERLAREACRRLTYVAAPISKLAFELGFEDPAYFCR
                     FFKRRTGHSPGAYRRATLAGASA"
     gene            complement(234..923)
                     /locus_tag="VNU48_02745"
     CDS             complement(234..923)
                     /locus_tag="VNU48_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWI10223.1"
                     /translation="MASQIKIISSMATRQVLTELVALYRQTTDQSIATESVGGVDAAR
                     RVRAGEAFDMVVLAGNAIDELIEAGRIVAGSRTDLVRSGVAIAVRSGSARIDIGSEAA
                     VKRAVAGARSISYSTGPSGVHLAKLFERWGIAEAIKDRIVQAPPGVPVGALIAQGKVE
                     LGFQQLSELIHLPGIDVLGPLPHEIQTVTTFAAGLATTSTQPDAVRALLRFMVSPAAA
                     GPKLNNGMEPA"
     gene            1177..2349
                     /gene="pobA"
                     /locus_tag="VNU48_02750"
     CDS             1177..2349
                     /gene="pobA"
                     /locus_tag="VNU48_02750"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HWI10224.1"
                     /translation="MRTQVGIIGGGPSGLLLSQLLHLQGIDTVVLERHSREYVLGRIR
                     AGVLEHGFAALMREARVAERMDREGEIHDGFFIAHDGIKERVDLKGLTGGNSVVVYGQ
                     TELTRDLYEGRDKLGGIVIHNAEDVQPHDLESARPYLTYRQGDEVERIDCDYVIGCDG
                     FHGVSRKSIPGDKITEFERAYPFGWLGVLSRTKPVSPELIYAKHDRGFALCSLRSQTL
                     SRYYIQVPLQDTVEQWSDALFWEELKKRLPHEVADALITGPSIEKSIAPLRSFVVEPM
                     RFGNLFLAGDAAHIVPPTGARGLNSAASDIHYLYHALVDHYKKRDDAGLDSYSQKALA
                     RVWKGQRFSWWMTTLLHRFPDSSAFDRKLQATDLAYLFSSQKALGSLAENYVGLPF"
     gene            complement(2429..2824)
                     /locus_tag="VNU48_02755"
     CDS             complement(2429..2824)
                     /locus_tag="VNU48_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI10225.1"
                     /translation="MLIVDDNAMNIAIAEVVLLAEKFAVETAVDGLDAIQKVASFGPD
                     LILMDIQMPGMNGLELTRQVKADPATRHIRIIAFTAFAMTGDEAKMRAAGCDGYLSKP
                     IDVAKFGAQVLAFLQVTTNDVPGKFRTAG"
     gene            complement(2836..5316)
                     /locus_tag="VNU48_02760"
     CDS             complement(2836..5316)
                     /locus_tag="VNU48_02760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWI10226.1"
                     /translation="MLEQDITIQAVHRVVAISRTGTRTPLEVRAALTRSDIDAEVTGL
                     AVLIRDMSRFAQAGAESSRLAAIVESSNDAIVGKTLDGRITSWNRAAQSIFGYSAKEA
                     IGQPVQMLIPPERQAEEMRILGKIAQGEPVPAFDTVRMTRSGERLDVQIAVSPIKDGA
                     GRVVGASKIARDVSRQRRAEAALRDSEEQLRFTLEAAHIGDWSLDLATGVMQHSGRHD
                     RCFGYTDVQDDWNFDRFIQHVRREDRADVEQTLHAAIAGLRDWRVQCRVVWPDDTVHW
                     ISMHGSVQRQGDKATRMLGIVTEITEQRQTEHARLAAQRLEAENRQFQEASRLKSQFL
                     ANMSHELRTPLNAVIGFADLLHSGLVKPDSPKHQEFLGHIGTSGRHLLQLINDVLDLS
                     KVESGKFQFYPEPLDLAVVLKEVQASLYTETTRKDIVISMQIDAGLADLMLDPARLKQ
                     VFSNYLSNAIKFTASGGNVTVRVVGQGPDHFRVEVEDTGIGISAADLPRLFTEFQQLD
                     AGYSRQHQGTGLGLALTRRLVEAQGGQVGVRSTVGAGSVFYVVLNRTHGLDSERAQAA
                     AQGLREREAERVLVIEDDLRDQALLVEALSQAGFEVDAAADGPYALRRARETAYGALT
                     LDLSLPGQRGLELLDHIRSEGASHAAPVVGVSMPADLEMSATFAIANVLCKPIRSDEI
                     SSAMARFKLPGTQRANVMVIDDKQAALDLMRATLKSIGIDAVCFQDGRVALQEIDRHR
                     PDAIVLDLMMPEFDGFQVLDALHRLPAWREVPVFIWTSMMLTDDEYATLARSARAILI
                     KGGGAMESVLESVRRWRHPAVAGVGVTS"
BASE COUNT          848 a         1797 c         1785 g          931 t
ORIGIN      
        1 cggcaaggcg gcactcgacc tggtccacga gcgcctcgcg cgcgaggcct gccggcgcct
       61 gacctatgtc gccgcgccga tctccaagct cgcgttcgag ctcggcttcg aggacccggc
      121 gtacttttgc cgcttcttca agcgccgcac cgggcacagc ccgggcgcct accggcgcgc
      181 cacgctggcg ggcgcgtcgg cctgaagagc ctgcgggccg cgggcgccgc ccgtcaggcg
      241 ggctccatgc cgttgttcag cttggggccg gcggcggcgg gcgacaccat gaagcgcagc
      301 agcgcgcgca ccgcgtcggg ctgagtcgag gtggtggcca ggcccgccgc gaaggtcgtg
      361 acggtctgga tctcgtgcgg cagcgggccg agcacgtcga tgcccggcag gtggatgagt
      421 tcgctgagtt gctggaagcc gagttcgacc ttgccctggg cgatcagcgc gccgaccgga
      481 acaccgggcg gggcctggac gatgcggtcc ttgatcgcct cggcgatgcc ccagcgctcg
      541 aacagtttcg ccaggtgcac accgctcggc ccggtcgaat agctgatgct tcgcgcgccc
      601 gcgacggcgc gcttgaccgc cgcttcggat ccgatgtcga tccgtgccga cccggagcgc
      661 accgcgatgg caacgccgga ccggaccagg tccgtgcggc tgccggccac gatcctgccg
      721 gcctcgatca gctcgtcgat ggcgttgccg gccagcacca ccatgtcgaa cgcttcgccg
      781 gcgcgcacgc gcctggccgc gtcgacgccg ccgacggatt cggtcgcgat cgactggtcg
      841 gtggtctggc ggtacagcgc gacgagttcg gtcagcacct gccgcgtcgc catcgacgag
      901 atgatcttga tctgcgacgc catgttctcg cctctccggg gtttgggtgg gtaatgtcga
      961 gtgccggtgc ccgacgcggg ctcagggccg gcgcggcgtt gtcgaacccg gcgccagcga
     1021 cactgtaaac ccgccgggtc gccattccgg gcgcgtcgca aaagtgcaag tctgcgcgga
     1081 catccgtcca actcggcgcg cgcgcttcgc cgcagaatcc ggttcagcgc cggtcgcgat
     1141 gcgagcgggc cgaatcgaac gacaggagac acggcgatga gaacccaggt cggaatcatt
     1201 ggcggcggcc cgtccggcct gctgctgtcg caactgctgc atctgcaggg catcgacacc
     1261 gtggtgctcg agcggcacag tcgtgaatac gtgcttgggc ggattcgcgc cggagtgctc
     1321 gagcacggct tcgccgcgct gatgcgcgag gcccgagtgg ccgagcggat ggaccgcgag
     1381 ggcgagatcc acgacgggtt cttcatcgcc cacgacggca tcaaggagcg ggtcgatctg
     1441 aagggcctga ccggcggcaa ctcggtcgtc gtctacggcc agaccgaact gacccgcgac
     1501 ctctacgaag gtcgggacaa gctcggcggc atcgtcatcc acaacgccga ggacgtgcag
     1561 ccgcacgacc tggagagcgc ccgcccgtac ctcacctacc gccagggtga cgaggtcgag
     1621 cgcatcgact gcgactacgt catcggctgc gacggcttcc acggcgtgag ccgcaagtcg
     1681 atacccgggg acaagatcac cgagttcgag cgtgcctacc cgttcggctg gctgggtgtc
     1741 ctgtcgcgca cgaagccggt gtcgcccgaa ctcatctacg ccaagcacga tcgcggcttt
     1801 gccctgtgct cgctgcgctc gcagacgctg agccgctact acatccaggt gccgctgcag
     1861 gacaccgtcg agcaatggtc ggacgcgttg ttctgggagg agctgaagaa gcggctgccg
     1921 cacgaggtgg ccgacgcgct gatcaccggc ccttccatcg aaaagagcat cgcgccgctg
     1981 cgctcgttcg tggtcgaacc gatgcgcttc ggcaatctgt tcctggccgg tgacgcggcc
     2041 cacatcgttc cgccgacggg cgcacgcggc ttgaacagcg cggcatccga catccactac
     2101 ctgtaccacg cgctggtcga ccactacaag aagcgcgacg acgccggtct ggacagctat
     2161 tcgcaaaagg cgctggcgcg ggtctggaag gggcagcgct tctcgtggtg gatgacgacg
     2221 ctgctgcatc gctttccgga cagcagcgcg ttcgatcgca agctgcaggc caccgacctg
     2281 gcctatctct tctcctcgca aaaggcgctc gggtcgctgg ccgagaacta cgtcggactg
     2341 cccttctagc gctaacggac ctgaatccgt ctaggtcgat aaacagcacc gccaacttgt
     2401 gctgactttg ggctgggact gtcgcatctc agccggcagt cctgaacttg ccaggaacgt
     2461 cattggttgt cacctgcaaa aaggcgagta cctgtgcgcc gaacttcgcc acatcgatcg
     2521 gcttggacag gtacccgtca catccggcgg cgcgcatctt ggcctcgtcg cccgtcatcg
     2581 cgaaggcggt gaaggcaata atgcggatgt gccgagtggc cgggtcggcc ttcacctgcc
     2641 gggtcagttc gagaccgttc atgccgggca tctggatgtc catgaggatc aggtccggcc
     2701 caaacgacgc caccttctga atcgcatcca ggccgtcaac ggcggtctcg accgcgaact
     2761 tctcggcaag cagcacgacc tcggcgatgg cgatgttcat cgcgttgtcg tcgacgatca
     2821 gcacccgggg cgcattcacg acgtcacccc gacgcccgcc acggcagggt ggcgccagcg
     2881 gcgcacgctc tccagcacgg attccatggc gccgccgcct ttgatcagga tggcgcgggc
     2941 cgatcgggcc agggtcgcgt attcgtcgtc ggtcagcatc atgctggtcc agatgaatac
     3001 cggtacctcg cgccaagcag gcaggcggtg caaggcgtcc agaacctgga agccatcgaa
     3061 ctcgggcatc atcaggtcca gcacgatggc gtcgggccgg tggcggtcga tttcctgcaa
     3121 ggcgacgcgg ccatcctgga aacagacggc gtcgatgccg atgctcttca gcgtcgcgcg
     3181 catcagatcg agcgctgcct gtttgtcgtc gatcaccatg acattggcgc gctgcgtgcc
     3241 cggcagcttg aagcgagcca tcgcggacga gatttcgtcg ctgcggatgg gtttgcacaa
     3301 gacgttggca atggcgaatg tggcgctcat ctccaagtcg gccggcatgc tgacgccgac
     3361 caccggtgct gcgtggctgg cgccctcgct ccgtatgtgg tcaagcagtt cgagcccgcg
     3421 ctggcccggc aggctcaggt cgagcgtcag cgccccgtac gcggtttcac gtgcgcgccg
     3481 cagcgcatac gggccgtcag cggccgcatc gacctcgaag cctgcttgcg agagggcctc
     3541 gacaagcagc gcctgatcgc gcagatcgtc ttcgatcacc agaacgcgct cggcctcgcg
     3601 ttcgcgcagg ccctgcgccg cggcctgcgc gcgctcactg tccaggccgt gggttcggtt
     3661 cagcacgaca taaaacacgc tgcccgcgcc caccgtgctg cgcaccccga cctggccacc
     3721 ttgtgcctcc accaaccggc gcgtcagcgc aaggccgagc cccgtgccct ggtgctgcct
     3781 gctgtatccc gcgtccagtt gctggaactc ggtgaacagg cgcggcaggt cggccgccga
     3841 gatgccgatg cccgtgtctt cgacctcgac ccggaagtgg tcggggcctt ggcccacgac
     3901 ccgtaccgtg acgttcccgc ccgatgccgt gaacttgatg gcgttcgaga ggtagttgga
     3961 gaacacctgc ttcagccgcg ccgggtcgag catgaggtcg gcgaggccgg catcgatttg
     4021 catcgatatc acgatgtcct tgcgagtggt ctccgtgtac aggctggctt gcacttcctt
     4081 caggacgacg gccaggtcca ggggctcggg gtagaactgg aatttgccgg actcgacctt
     4141 ggacaggtcg agcacatcgt tgatcaactg cagcaggtga cgcccgctgg taccgatgtg
     4201 gccgaggaat tcctgatgct tgggcgagtc tggcttgacc aagcccgaat gcagcaggtc
     4261 cgcgaacccg atcactgcgt tgagcggcgt gcgcagctcg tgggacatgt tggccaggaa
     4321 ctggctcttg agccggctgg cctcctgaaa ctgccggttc tcggcttcga gccgctgggc
     4381 ggccagccgc gcgtgctcgg tctgccgctg ctcggtgatc tcggtgacga tgccgagcat
     4441 gcgggtggcc ttgtcgcctt ggcgctgcac actgccatgc atgctgatcc agtgaaccgt
     4501 gtcatcaggc cagaccaccc ggcactggac gcgccagtcg cgcaacccgg cgatggccgc
     4561 gtggagcgtc tgctcgacgt cggcacggtc ttcccgacgc acgtgctgga tgaacctgtc
     4621 gaagttccag tcgtcttgca cgtcggtgta accgaagcat cggtcgtggc gcccggagtg
     4681 ctgcatgacg ccggtggcga ggtccaggct ccagtcgccg atgtgagccg cctcgagcgt
     4741 gaaccgcagc tgctcctcgc tgtcgcgcaa ggcggcttcg gcccgacgct ggcgcgagac
     4801 atcgcgtgcg atcttggagg ccccgacgac gcggcctgcg ccgtccttga tcggcgagac
     4861 agcgatctgc acgtccagcc tttcaccgct gcgcgtcatc cgcacggtgt cgaaggccgg
     4921 tacgggttcg ccctgcgcga ttttgccgag gatgcgcatc tcttcggcct gccgctccgg
     4981 cgggatcaac atctgcaccg gctgcccgat tgcttccttg gcgctgtagc cgaagatcga
     5041 ttgcgcggcg cggttccagc tggtgatgcg cccgtccagc gtcttgccga cgatcgcgtc
     5101 gttggacgat tcgacgatgg cggcgagccg gctcgattcg gccccggcct gggcgaagcg
     5161 cgacatgtcc cggatcaaga cggccagacc ggtgacctct gcgtcgatgt cactgcgcgt
     5221 cagggccgcc ctcacttcca gcggggtgcg cgttccggtg cgcgaaatgg ccacaacccg
     5281 gtgaactgcc tggatggtga tgtcctgttc cagcatcaca tccacagggc tcttcgagag
     5341 gtaggctacc ggccgtcctt c
//
LOCUS       DATMJB010000123         6948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_101600, whole genome shotgun sequence.
ACCESSION   DATMJB010000123 DATMJB010000000
VERSION     DATMJB010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6948
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101600"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VNU48_02765"
     CDS             <1..256
                     /locus_tag="VNU48_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10227.1"
                     /translation="GDDADPGHRTMVMTTLFNLKGLALRTGAGPLYALSDALRGNYAD
                     ERAGKPVAPRGPLVADLLRECERTRAALLACGASPDTFGG"
     gene            311..556
                     /locus_tag="VNU48_02770"
     CDS             311..556
                     /locus_tag="VNU48_02770"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HWI10228.1"
                     /translation="MKMVEQPPVTCSFCRRTSHDTGVDVMVTLQDHSAAICGECVEIA
                     RDILGFVRHGHAVETVAHPALPTAPFKVVTSGNGRQQ"
     gene            complement(566..802)
                     /locus_tag="VNU48_02775"
     CDS             complement(566..802)
                     /locus_tag="VNU48_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10229.1"
                     /translation="MQPPLTTEIAPAPKRMKRLAVIARVAAPKLALAFAAAGLTLTII
                     NPPGIGVVLMFTGVVAYLILADMRLRTRSKAGDN"
     gene            complement(1057..1392)
                     /gene="fliE"
                     /locus_tag="VNU48_02780"
     CDS             complement(1057..1392)
                     /gene="fliE"
                     /locus_tag="VNU48_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HWI10230.1"
                     /translation="MTITMNVTLKPFDFAQAVARAGLASNGQPLQKNAAVEGAGFQQA
                     LTQALGAVSKSQNDVTAMQREVQLDNPNVSIEQTMVAMQKAQIGFQAALQVRNKMVQA
                     YTDIMQMQV"
     gene            1530..3200
                     /gene="fliF"
                     /locus_tag="VNU48_02785"
     CDS             1530..3200
                     /gene="fliF"
                     /locus_tag="VNU48_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HWI10231.1"
                     /translation="MNDAVAINPALPLVEPASFAGRMAALPLRSKLSAAIGVAALAGV
                     VLAMTLWSSKGDFKVLYANLSDKDGGAVIAQLSQMNVPYRMSEGGSAILVPALQVHDL
                     RLKLATAGLPKGSVSGYELMDTARFGQTQFQERLTAQRGLEGELTRSITSLAAVANAR
                     VHLALPNQNGFFREQQKPSASVLLSLHPGRTLDRAQVAGIVHLVSSSVPELDPKAVSV
                     LDQSGALLTSSGDGQPSAGLDAQQLQYVNQIETGYGKRIVELLEPIVGRDNLRATVAA
                     DIDFSQTEATSEEFKPNQGAQAQVSIRSQQVSEQNGSTGAQPSGVPGAASNQPPVPAT
                     APLVGAAQPLQTAQAGGGAGTSGRRDATTNYEVDKTVRVTRNASGNIKRLNAAVVVNN
                     RSVTDAKGKTTQVPLSGEELDKLTALVRESIGFNKERGDSVKVINAPFKVEPITNVEL
                     PLWKQPGTIDLLRSLAVPAGLALMGLLVFFGLVRPALKAALAPPPAPAPGAKLSAVVD
                     DSQELPALPAPQSFQHLAGARALAKDNPAAVAGIVRGWVNGEAVPAKA"
     gene            3240..4238
                     /gene="fliG"
                     /locus_tag="VNU48_02790"
     CDS             3240..4238
                     /gene="fliG"
                     /locus_tag="VNU48_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HWI10232.1"
                     /translation="MDDQGLENAAILLMSLGEEEAASVFKHLSPKEVQGLGETIQKMK
                     SIPRERVDKVLALFHNVAAEQSNLVSDTDEYVKSVLRKALGDDKANLLIDRIMTGGGD
                     TAGIESLKWMDPASVGELLRIEHPQIVAAILVHLDFDQAAEVLKTFTERQRNEVLVRV
                     ATLDGIQPSALKDLNEVMGRVLAGGERTRKTALGGVKAAAEMINLMGTSVETAVLDYI
                     READNDLAQKIMDNMFTFDDLEKIDDKGIQALLKEVQSESLVIALKGATPEMREKIFK
                     NMSTRAAETLREDLESRGPVRVSEVEAEQKELLKVVRRLADEGQIVLGGGGDDDFL"
     gene            4225..4971
                     /locus_tag="VNU48_02795"
     CDS             4225..4971
                     /locus_tag="VNU48_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HWI10233.1"
                     /translation="MTSSEPRKNIPSPNEAARGAYARFIPREELGGFAAWQPGDLSGG
                     PARQANVQRAPDEMPKADAAAELAAQLKAARQSGYQDGYRDGLVALDGFKQSFAAQTT
                     AQIGTLLRALDDELGGLQQDMARSLALAATQLARQIVRAELASQPVLITKIAQEAIDT
                     LLLSAKHITLRVHPDDQPFVAQGAGEVLAARGARLLADTSMSRGGCVVESDIGVIDAS
                     LETRWRRAAASLGCDDAWDANAAADTDAPA"
     gene            4968..6392
                     /gene="fliI"
                     /locus_tag="VNU48_02800"
     CDS             4968..6392
                     /gene="fliI"
                     /locus_tag="VNU48_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HWI10234.1"
                     /translation="MIRTRQETLPLANAEKWRRYLADMRSFSADQVALETQGLLVRVT
                     GLVLEAAGIRVPVGSVCEVRMEGQTPVIAEVVGFSGDRAYLMPTGDVAGLMSGARVVP
                     RPSPVVPMKLGAANHPWRRNEDRTLHLPVGDGLLGRVVDPHGHPMDRRGPIDHVHTEP
                     LTRRPINAMDRDPVRTPLDTGVRAINAMLTVGRGQRIGLFAGTGVGKSVLLGMMARYT
                     AADVIVVGLIGERGREVKEFIEDILGVEGMRRSVVVAAPADAPPLVRMQGANYATVIA
                     EHFRDRGLNVLLLMDSLTRYAMAQREIALAIGEPPATKGYPPSCFAKLPQLVERSGNG
                     VAGGGSITAIYTVLSEGDDQQDPIADAARGILDGHIVLSRELAEAGHYPAIDIEKSVS
                     RVMTSVATGEHVESARRFRQLHARHNKARDLIQLGAYAPGHDNELDTAVRLHDPMTAL
                     LQQGMHESAALDASVRQLRAVIAH"
     gene            6501..>6948
                     /gene="fliJ"
                     /locus_tag="VNU48_02805"
     CDS             6501..>6948
                     /gene="fliJ"
                     /locus_tag="VNU48_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HWI10235.1"
                     /translation="MTDLQPLTILLGQSERQRDAALAEHQRAQLASDAAAAQAEQLRT
                     YRREYEQRWSAQFARRGQIELVHCYQSFMERLTQAVEQQARMAELAAQRLAHALAALR
                     SAEMRCASVRKMIERRVLEQRAATERREQKQTDEAATRAAWVRIGAT"
BASE COUNT         1164 a         2465 c         2384 g          935 t
ORIGIN      
        1 gggcgacgac gcagacccgg gccacagaac gatggtgatg acgaccctgt tcaacctcaa
       61 gggcctcgca ttgcggacgg gcgcaggccc gctctacgca ctgtccgacg ccctgcgcgg
      121 caactacgcg gacgagagag ccggcaagcc cgtagcaccc cgcgggcctc tggtggccga
      181 cttgttgcgc gaatgcgagc gcacccgggc ggcgctgctg gcttgcggag cgtcgcccga
      241 taccttcggc gggtagggca tagccactct ggcacaattt gttacttggg ctccgcacaa
      301 aggtgtgtcg atgaaaatgg tcgagcagcc gcccgtgacc tgcagctttt gccgccgcac
      361 gagccacgac accggcgttg atgtgatggt caccctgcaa gaccacagcg ccgcgatctg
      421 cggcgagtgc gtcgagatcg cccgcgacat tctcggtttc gtgcgacatg gacacgccgt
      481 cgaaaccgtc gcacaccccg cgttgccgac cgccccgttc aaggtcgtta cgagcgggaa
      541 tggccggcag caatagcccg gagagtcagt tgtcgccggc cttgcttcgc gtgcgcaggc
      601 gcatgtcggc caagatcaag taagcaacta cccctgtgaa catcagaacc acgccgattc
      661 cgggtgggtt gatgatcgtc agggtgagtc cggccgcggc gaaggcaagt gcgagcttgg
      721 gcgccgcaac ccgggctatc accgccagcc gtttcatgcg ctttggcgct ggcgcgatct
      781 cggtcgtgag tggtggttgc atagagtgcc tcgcgggtgt gacgcgatgc actcagcata
      841 gtcctcgttt ggcgtcggcc tctgttcggt agcgaactta gcgtgcaaag tcgtgtaagg
      901 cattggtcga ccgccaccac cgccctgaga acgtcaggcg cggcctgcgt gatagtggcg
      961 acacactgcc tcacgaattc gagctcgagc gcgctttgcg cggcggcttc cgtagcgatc
     1021 accctacgcc catggcgccg aggcttgccc ggccggctac acctgcatct gcatgatgtc
     1081 ggtataggcc tggaccatct tgttgcgcac ctgcagcgcc gcctgaaagc cgatctgcgc
     1141 cttctgcatc gccaccatcg tctgctcgat gctgacgttc gggttgtcga gctgcacctc
     1201 gcgctgcatc gcggtcacgt cgttctgcga cttgctgacc gcgccgagcg cctgcgtcag
     1261 cgcctgctgg aagcccgcgc cctcgaccgc cgcgttcttc tgcagcggct ggccattgct
     1321 ggccagcccg gcgcgtgcca cggcctgggc gaagtcgaag ggtttgaggg tcacgttcat
     1381 cgtgatcgtc atgctaagca tccgggcgcg ccgcgtttgc gcgaactgac gccctattga
     1441 cgccctgttc gcagcatccg gcgcacccga gttcccgaac aatcgctttc aatcgaggcc
     1501 cacccggccc ggacggaacc cgacgaccca tgaacgacgc agtcgccatc aaccccgccc
     1561 ttcccctggt cgaacccgcc agcttcgccg gccgcatggc cgcgctgccg ctgcgcagca
     1621 agctcagcgc cgcgatcggt gtcgcggcgc tggccggtgt cgtgctggcg atgacgctgt
     1681 ggagcagcaa gggcgacttc aaggtgctgt acgcgaacct gtccgacaag gacggcggcg
     1741 ccgtgatcgc ccagctctcg cagatgaacg tgccctaccg catgagcgaa ggcggcagcg
     1801 cgatcctggt gccggcgctg caggtgcacg acctgcgcct gaagctcgcc accgccgggc
     1861 tgccgaaggg atcggtctcg ggctacgagc tgatggacac ggcgcgcttc ggccagaccc
     1921 agttccagga gcgcctgacc gcgcagcgcg gcctcgaggg cgagctgacg cgctcgatca
     1981 cctcgctggc cgcggtcgcg aatgcccgcg tgcacctcgc gctgccgaac cagaacggct
     2041 tcttccgcga gcagcagaag cccagcgcct cggtgctgct gagcctgcac ccgggccgca
     2101 cgctggaccg cgcccaggtc gccggcatcg tgcacctggt ctccagcagc gtgcccgagc
     2161 tcgacccgaa ggccgtgagc gtgctcgacc agagcggcgc gctgctcacc agcagcggcg
     2221 acggccagcc ctcggcgggc ctggacgcgc agcagctcca gtacgtgaac cagatcgaga
     2281 ccggttacgg caagcgcatc gtcgagctgc tcgagccgat cgtcgggcgc gacaacctgc
     2341 gcgccacggt cgccgccgac atcgacttct cgcagaccga ggcgacctcg gaagagttca
     2401 agccgaacca gggcgcgcag gcccaggtct cgatccgcag ccagcaggtc tctgagcaga
     2461 acgggtccac cggcgcgcag cccagcggcg tgccgggcgc ggcctcgaac cagccgccgg
     2521 tgccggcgac cgcgccgctg gtcggcgccg cgcaaccctt gcagaccgcg caggcgggcg
     2581 gcggcgccgg caccagcggg cgccgcgacg cgaccaccaa ctacgaggtc gacaagaccg
     2641 tgcgcgtgac gcgcaacgcc agcggcaaca tcaagcggct gaacgccgcg gtcgtcgtca
     2701 acaaccgctc ggtgaccgac gcgaagggca agaccacgca ggtgccgctg tccggcgagg
     2761 agctcgacaa gctgaccgcg ctggtgcgcg agagcatcgg cttcaacaag gagcgcggcg
     2821 actcggtcaa ggtgatcaac gcgccgttca aggtcgagcc gatcacgaac gtcgagctgc
     2881 cgctgtggaa gcagccgggc acgatcgacc tgctgcgcag cctggccgtg ccggccggcc
     2941 tggcgctgat ggggctgctg gtgttcttcg gccttgtccg cccggccctg aaggccgcgc
     3001 tggcgccgcc gccggcaccg gcgcccggcg cgaagctgag cgccgtcgtc gacgactccc
     3061 aggagctgcc ggcgctgccg gcgccgcaga gcttccagca cctggccggc gcgcgcgcgc
     3121 tggccaagga caacccggcc gcggtggcgg gcatcgtgcg cggctgggtc aatggcgagg
     3181 ccgtgccggc caaggcctga cgcgcacggc ggcagaacaa gaaatccaag gagcagtacg
     3241 tggacgacca aggcctggag aacgcagcga tcctgctgat gtcgctcggc gaagaggaag
     3301 ccgccagcgt cttcaagcac ctcagcccga aggaggtgca gggcctgggc gagacgatcc
     3361 agaagatgaa gtcgatcccg cgcgagcgcg tcgacaaggt gctggcgctg ttccacaacg
     3421 tcgccgccga gcagagcaac ctcgtctccg acaccgacga gtacgtcaag agcgtgctgc
     3481 gcaaggccct gggcgacgac aaggcgaacc tgctgatcga ccgcatcatg accggcggcg
     3541 gcgacaccgc cggcatcgag agcctgaagt ggatggaccc ggcctcggtc ggcgagctgc
     3601 tgcgcatcga gcacccgcag atcgtcgcgg cgatcctcgt gcacctcgac ttcgaccagg
     3661 ccgccgaggt gctgaagact ttcaccgaac gccagcgcaa cgaggtgctg gtgcgcgtgg
     3721 ccacgctcga cggcatccag ccctcggcgc tgaaggacct gaacgaggtg atgggccgcg
     3781 tgctggccgg cggcgagcgc acgcgcaaga cggcgctggg cggcgtcaag gcggcggccg
     3841 agatgatcaa cctgatgggc acgagcgtcg agaccgcggt gctcgactac atccgcgaag
     3901 ccgacaacga cctggcgcag aagatcatgg acaacatgtt caccttcgac gacctggaga
     3961 agatcgacga caagggcatc caggcactgc tcaaggaagt gcagtccgaa tcgctggtga
     4021 tcgcgctgaa gggcgcgacg ccggagatgc gcgagaagat cttcaagaac atgtccacgc
     4081 gcgccgccga gacgttgcgc gaagacctgg aatcgcgcgg cccggtgcgc gtctccgagg
     4141 tcgaggccga gcagaaggaa ctgctgaagg tggtgcgccg cctggccgac gaaggccaga
     4201 tcgtcctggg cggcggaggc gacgatgact tcctctgagc cgcgcaagaa catcccgtcg
     4261 ccgaacgagg ccgcacgcgg cgcctacgcg cgcttcatcc cgcgcgagga actcggcggc
     4321 ttcgccgcct ggcagcccgg cgacctgagc ggcggcccgg cgcggcaggc caatgtgcag
     4381 cgcgcacccg acgaaatgcc caaggccgac gccgccgccg aactggccgc gcagctcaag
     4441 gccgcacgcc agtccggata tcaagacggc taccgcgacg gcctggttgc gctcgacggc
     4501 ttcaagcaga gcttcgcggc ccagaccacg gcccagatcg gcacactgct gcgcgcgctc
     4561 gacgacgagc tcggcggcct gcagcaggac atggcgcgct cgctggcgct cgccgccacg
     4621 cagctggcgc gccagatcgt gcgcgccgaa ctcgcgagcc agccggtgct gatcaccaag
     4681 atcgcgcagg aggcgatcga cacgctgctg ctcagcgcga agcacatcac gctgcgcgtt
     4741 caccccgacg accagccctt cgtcgcgcag ggcgcgggcg aggtgctggc cgcacgcggc
     4801 gcacggctgc tcgccgacac ctcgatgtcg cgcggcggct gcgtcgtcga atcggacatc
     4861 ggcgtgatcg acgccagcct cgagacacgc tggcgccgcg ccgccgcgtc gctgggttgc
     4921 gacgacgcct gggacgcgaa cgcggcggcc gacacggacg cgccggcatg atccgcacgc
     4981 gccaggaaac actgccgctg gcgaacgccg agaaatggcg tcgctacctc gccgacatgc
     5041 gcagcttctc ggccgaccag gtcgcgctcg agacgcaggg cctgctggta cgcgtgaccg
     5101 gcctcgtgct cgaggcggcc ggcatccgcg tgccggtcgg ctcggtctgc gaggtgcgca
     5161 tggaaggcca gacgccggtc atcgccgagg tcgtcggctt ctcgggcgac cgcgcctacc
     5221 tgatgcccac cggcgacgtt gccggcctga tgagcggcgc gcgcgtcgtg ccgcgaccgt
     5281 cgccggtcgt gccgatgaag ctcggcgccg cgaaccaccc gtggcgccgc aacgaggacc
     5341 gcacgctgca cctgccggtc ggcgacggcc tgctcggccg cgtcgtcgat ccgcacggcc
     5401 acccgatgga ccggcgcggc ccgatcgacc acgtgcacac cgagccgctg acgcgccgcc
     5461 cgatcaacgc gatggaccgc gacccggtgc gcacgccgct ggacaccggc gtgcgtgcga
     5521 tcaacgcgat gctcaccgtc gggcgcggcc agcgcatcgg cctgttcgcc ggcaccggcg
     5581 tgggcaagtc ggtgctgctc gggatgatgg cgcgctacac cgcggccgac gtgatcgtcg
     5641 tcgggctgat cggcgagcgc ggccgcgagg tcaaggaatt catcgaggac atcctcggcg
     5701 tcgagggcat gcgccgctcc gtcgtcgtcg ccgcgcccgc cgacgcaccg ccgctggtgc
     5761 gcatgcaggg cgcgaactac gccaccgtga tcgccgagca ctttcgcgac cgcgggctga
     5821 acgtgctgct gctgatggac tcgctgacgc gctacgcgat ggcgcaacgc gagatcgcgc
     5881 tggcgatcgg cgagccgccc gcgaccaagg gctacccgcc gagctgcttc gcgaagctgc
     5941 cgcagctggt cgagcgcagc ggcaacggcg tggctggcgg cggctcgatc accgcgatct
     6001 acaccgtgct gtccgagggc gacgaccagc aggacccgat cgccgatgcc gcgcgcggca
     6061 tcctcgacgg ccacatcgtg ctgtcgcgcg aactcgccga ggccggccac tacccggcga
     6121 tcgacatcga gaagtcggtg tcgcgggtga tgaccagcgt cgccaccggc gagcacgtcg
     6181 agtcggcgcg gcgcttccgc cagctccacg cacgccacaa caaggcacgc gacctgatcc
     6241 agctcggcgc ctacgcaccc ggccacgaca acgaactcga caccgcggtg cgcctgcacg
     6301 acccgatgac ggcgctgctg cagcagggca tgcacgagag cgcggcgctc gacgccagcg
     6361 tgcgccagct gcgcgcggtg atcgcgcatt gaggaaccaa atgcctacga cttcttgccg
     6421 ggccgagcgc cgggccgctc ccaagccggc ccgcatcccc tcgggggatc gaccgatgta
     6481 ctcatcgggc gaggggctcc atgactgatc tgcaaccgtt gacgatcctg ctgggccaga
     6541 gcgaacgcca gcgggacgcg gcgctcgccg agcaccagcg cgcgcagctc gccagcgacg
     6601 ccgccgccgc ccaggccgag cagctgcgca cctaccggcg cgaatacgag cagcgctgga
     6661 gcgcgcagtt cgcgcgccgc ggccagatcg aactcgtgca ctgctaccag agcttcatgg
     6721 aacggctgac ccaggcggtc gaacagcagg cgcgcatggc cgaactcgcg gcgcagcggc
     6781 tcgcccacgc gctggccgcg ctgcgctcgg ccgagatgcg ctgtgcctcg gtgcgcaaga
     6841 tgatcgagcg gcgcgtgctc gagcagcgtg ccgccaccga gcgccgcgag cagaaacaga
     6901 ccgacgaggc cgccacgcgc gcggcctggg tgcgcatcgg cgccacgc
//
LOCUS       DATMJB010000124         9678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_457031, whole genome shotgun sequence.
ACCESSION   DATMJB010000124 DATMJB010000000
VERSION     DATMJB010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9678
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457031"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2251
                     /locus_tag="VNU48_02810"
     CDS             <1..2251
                     /locus_tag="VNU48_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HWI10236.1"
                     /translation="VAMYIGSNAAEAHPVSMLHMLNAKENGCKMIVVDPRFTRTAAKA
                     DEYVRIRSGTDIAFLFGVLYHVFKNGWEDKKYIADRVYGMDKVREDVLAKWAPDKVLE
                     ACGVDEATCYQAAKTMAENRPSTIVWCMGQTQHTIGNAIVRASCILQLALGNIGVSGG
                     GANIFRGHDNVQGATDVGPNPDSLPGYYGLAEGAWKHFAKVWDVDFEWIKKQYAPGML
                     TKPGMTVSRWIDGVLEKNELIDQDSNLRGLFFWGHAPNSQTRGLEMKKAMDKLDLLVV
                     IDPYPSATAAMAAMPGKPEDLNPNRAVYLLPAASQFETSGSVTASNRSLQWREKVIEP
                     LWDSRTDHMIMYQLAQKLGFDKEFTKSHKLVAGKGGMMEPEPESVLREINRGMWTIGY
                     TGQSPERLKAHMRNMNVFDVKTLKAKGGVDKETGYQLDGDYFGLPWPCYGTPEIKHPG
                     SPNLYDTSKSMMEGGGNFRANFGVEKDGVNLLAEDGSHSKDAQITTGYPEFDHILLKK
                     LGWWNDLTDAEKKAAEGKNWKTDLSGGIQRVTMARGCHPWGNAKARAVVWNFPDPIPQ
                     HREALYSTRPDLVAKYPTHDDKKAFWRLPTLFKSVQEKNKDIGKTFPLIMSSGRLVEY
                     EGGGEETRSNPWLAELQQEMFVEINPATANDRGIRDGENVWVTTPTGAKITVKALVTE
                     RVDRETVWLPFHFSGRWQGADMLAYYPEGAHPVIRGEAVNTATTYGYDSVTMMQETKT
                     TVCQVQRAT"
     gene            2372..3004
                     /gene="fdh3B"
                     /locus_tag="VNU48_02815"
     CDS             2372..3004
                     /gene="fdh3B"
                     /locus_tag="VNU48_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HWI10237.1"
                     /translation="MARMKFICDAERCIECNGCVTACKAEHNVPWGVNRRRVVTLNDG
                     VAGEKSISVACMHCSDAPCMAVCPVDCFYRTEEGVVLHDKDVCIGCGYCSYACPFGAP
                     QFPSNGEFGLRGKMDKCTFCAGGPEANGSEAENLKYGRNRLSEGKLPACAEMCSTKAL
                     LGGDGDVVADIFRTRVLNRGKGSEVWGWGTAYGKPVQAGSAASPASEVKK"
     gene            3001..3240
                     /locus_tag="VNU48_02820"
     CDS             3001..3240
                     /locus_tag="VNU48_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10238.1"
                     /translation="MIARALALVAATALTLSLAACGEKPQRMDASARKADAAPWTVSD
                     AANPAFKAPGWKDGDQAAWEQQIRQRNQAQNDYAR"
     gene            3261..4421
                     /locus_tag="VNU48_02825"
     CDS             3261..4421
                     /locus_tag="VNU48_02825"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HWI10239.1"
                     /translation="MRSPLFRLSSFRCTPRVLAGLLAGLAVAFAGAQTAPGAAASATA
                     GAPAGFVAPAEPKADETNAQRAKSQPGNNAPFWRSVHDSGNRPGSVNNMVSTDRGVLI
                     QEFVQYPGSRVTNAGEAWRQVRNGWIIPWGGALVVIMVLAIALFYFAKGPLGGHVPYT
                     GRKIERFTYFERAAHWSNAIAFCVLAISGLVMAFGKFWLLPVIGATLFGWLTYALKTA
                     HNFAGPLFAVSLVIVFVTFLRDNFPSRADWQWLRRGGGLFGGEEPASARFNAGEKLIF
                     WGGVFVLGIVVVASGLVLDKLVPGLGYLRGDMQIANMVHGVATVLMMAMFLGHIYIGT
                     IGMKDAYGAMKTGYVDEGWAREHHALWYEDIKAGRITAQRTAPAEGRPAAQA"
     gene            4444..4815
                     /locus_tag="VNU48_02830"
     CDS             4444..4815
                     /locus_tag="VNU48_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10240.1"
                     /translation="MRLAIPALIVGTALALPAFAKLPAPSDDAKAKAAAAAAKTAWTD
                     KVGAYQLCRAMERTAEYYRKSENGKQAPAAIDTPPCTDPGPFVAPPPLEAAGAHSPAT
                     MATSPPSSTATAAEQKGQPKK"
     gene            4886..5746
                     /locus_tag="VNU48_02835"
     CDS             4886..5746
                     /locus_tag="VNU48_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWI10241.1"
                     /translation="MSIAPRLTTATAALMHEVDVLDEFGATRRISIPAERALTVFVDN
                     RELVTLMTLGAAPELLVLGYLRNQRLVESVDDIDSITVDWDVQAAAVKTRAGIERFDE
                     KTAKRVVTTGCGQGTVFGSLMDDLGAIRLPAADEPAARISQHALYGLLNAMRLQESTY
                     KSAGSVHGCALFRQDELLMFVEDVGRHNAIDTIAGWMWLNFVAGGDKIFYTTGRLTSE
                     MVIKSAQMGVPIIVSRSGITQMGHQLAHQLGLALFGRATNRHFICYSGFDRFDSQPEP
                     ARGVAGNVGA"
     gene            complement(5667..6347)
                     /locus_tag="VNU48_02840"
     CDS             complement(5667..6347)
                     /locus_tag="VNU48_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWI10242.1"
                     /translation="MDKRRFLAAAALGAALPLGARAAPAAGRTGPGLLTVAGAITHSN
                     RGPLDPALDQLMVKHGTQFGKAREFDAAMLARLPSVTIQPTLEYDAKVHKLSGPLLGS
                     VLAAAGVAADAHVMVALRALDGYNVALSLADAHRYRMIVATALDGAPLSIGGLGPLWA
                     VYDADRLPDFKDKPLKERFGLCPWGLYFIEVKTVCEVGHHATRPRCLRRRAPARAANR
                     SDRSRCSR"
     gene            6406..6768
                     /locus_tag="VNU48_02845"
     CDS             6406..6768
                     /locus_tag="VNU48_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10243.1"
                     /translation="MHARRGTKLATRLVRHRDGRQTGPRRAFYQPNVSILWTEMVRAF
                     QKFQMRVRAVAEVLVGAMLGMIPALLPAGAFVALADISKDSESFMLAMLYAGSVGGCA
                     VGIAFGKRRAAKRHAAHD"
     gene            6752..7525
                     /locus_tag="VNU48_02850"
     CDS             6752..7525
                     /locus_tag="VNU48_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWI10244.1"
                     /translation="MPRTTDRPVRILLVSDLHYTLPQLDWVVRSAPSFDLVVLAGDQL
                     DISSTVSLDAQSVVMLRYLALLQAAGRVVVSSGNHDLTGPDANGEQSALWLGKARAAG
                     VPTDGDSLRVGDTLITICPWWDGPQGRLALEAQLAADARRRPARWLWVYHWPPLGTKT
                     CWTGRRDYGDPDLVGWIAQHRPEVVLTGHVHESAFKPTGCWADRVGDTWIFNAGRQIG
                     PVPTHIELDLDAGSASWRSMLGVEDMRLSDTHAPARTVF"
     gene            complement(7537..8019)
                     /locus_tag="VNU48_02855"
     CDS             complement(7537..8019)
                     /locus_tag="VNU48_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWI10245.1"
                     /translation="MNDMLALSEGLPEVAFARGETVVREGGAAGGIWVLVSGDLQVRK
                     GDSVVNTVSRPGALVGEVSVLLDTVYSATVEATEPSVLRYAADGRALLTRDPAITLLV
                     AVGLAERLNFVTTYLADLKHQYGDAPGLAMVSDVMRELAQLQGPSARAGSARDPEPEY
                     "
     gene            complement(8100..9488)
                     /locus_tag="VNU48_02860"
     CDS             complement(8100..9488)
                     /locus_tag="VNU48_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HWI10246.1"
                     /translation="MNFPKTLCSSATLALCALFAAPADAIDLIAIGQLPGTVFDHSGQ
                     SALLENGVRGDLLGGIGSGLAWAGGETFLALPDRGPNAVAWNAAVNDTTGYVPRFHTL
                     QLSLQPGPDAASGLPFTLTPTLVATTLLFSREPLNYGASVAGYRAEPAANTASRHYLT
                     GRSDNFDPATSSADTRDARLDPEGIRVSANGRFVYISDEYGPYLYQFDRASGARVRAI
                     KLPANLAIAHKSPMGAVEISGNTSGRVANKGMEGLAISPDGQRLFGFVQSPLIQDGGD
                     GGRANRIVTIDLESDEVHQYAYDNFLADKAKAFNSSEVLALNEHELLVLERDGKGLGD
                     DSKAVVKRIYKIDLAGATDVSALSGEAALLSHAVGKSLFLDIAAKLTAAGLTAEQIPA
                     KLEGMAFGADVVIDGVVKHTLYIGNDNDFLAVTPGGRTNPNQWFVFAFTEADLGGSAF
                     VNQQWSGAAAGR"
BASE COUNT         1559 a         3309 c         3389 g         1421 t
ORIGIN      
        1 ggtcgcgatg tacatcggct ccaacgcggc cgaggcgcat ccggtgagca tgctgcacat
       61 gctgaatgcc aaggaaaacg gctgcaagat gatcgtggtc gatccgcgct tcacccgcac
      121 ggcggccaag gccgacgagt acgtgcgcat ccgctccggc actgacatcg ccttcctgtt
      181 cggcgtgctg taccacgtgt tcaagaacgg ctgggaagac aagaagtaca tcgccgaccg
      241 cgtctacggc atggacaagg tgcgcgagga cgtgctcgcg aagtgggcgc ccgacaaggt
      301 gctcgaggcc tgcggcgtcg acgaggccac ctgctaccag gccgcgaaga cgatggccga
      361 gaaccggccc agcacgatcg tctggtgcat gggccagacc cagcacacga tcggcaacgc
      421 gatcgtgcgc gcctcgtgca tcctgcagct cgcgctcggc aacatcggcg tcagcggcgg
      481 cggcgccaac atcttccgcg gccacgacaa cgtgcagggc gcgaccgacg tcggcccgaa
      541 ccccgattcg ctgcccggct actacggcct ggccgagggc gcctggaagc acttcgcgaa
      601 ggtctgggac gtcgacttcg agtggatcaa gaagcagtac gcgcccggca tgctgaccaa
      661 gcccggcatg acggtgtcgc gctggatcga cggcgtgctc gagaagaacg agctgatcga
      721 ccaggactcg aacctgcgcg gcctgttctt ctggggccac gcgccgaact cgcagacgcg
      781 cggtctcgag atgaagaagg ccatggacaa gctcgacctg ctggtggtga tcgacccgta
      841 cccgtcggcg accgcggcga tggcggcgat gcctggcaag cccgaagacc tgaacccgaa
      901 ccgcgcggtc tacctgctgc cggcggcaag ccagttcgag acctcgggct cggtgaccgc
      961 gtcgaaccgc tcgctgcaat ggcgcgagaa ggtgatcgag ccgctctggg actcgcgcac
     1021 cgaccacatg atcatgtacc aactcgcgca aaagctgggc ttcgacaagg agttcacgaa
     1081 gagccacaag ctggtggcgg gcaagggcgg gatgatggaa cccgagcccg agtcggtgct
     1141 gcgcgagatc aaccgcggca tgtggacgat cggctacacc ggccagagcc ccgagcggct
     1201 gaaggcgcac atgcgcaaca tgaatgtctt cgacgtgaag acgctcaagg ccaagggcgg
     1261 cgtcgacaag gaaaccggct accaactcga cggcgactat ttcggcctgc cgtggccgtg
     1321 ctacggcacg cccgagatca agcatccggg ctcgccgaac ctgtacgaca cctcgaagtc
     1381 gatgatggaa ggcggcggca acttccgcgc caacttcggg gtcgagaagg acggcgtcaa
     1441 cctgctggcc gaagacggtt cgcattcgaa ggacgcgcag atcaccaccg gctaccccga
     1501 gttcgaccac atcctgctga agaagctcgg ctggtggaac gacctgaccg atgccgagaa
     1561 gaaggcggcc gagggcaaga actggaagac cgatctgtcg ggcggcatcc agcgcgtgac
     1621 gatggcgcgc ggctgtcacc cctggggcaa tgcgaaggcg cgcgcggtgg tctggaactt
     1681 ccccgacccg atcccgcagc accgcgaggc gctgtactcg acccggcccg acctcgtcgc
     1741 caagtacccg acccacgacg acaagaaggc gttctggcgc ctgcccacgc tgttcaagag
     1801 cgtgcaggag aagaacaagg acatcggcaa gaccttcccg ctgatcatga gttcggggcg
     1861 tctggtcgag tacgaaggcg gcggcgagga aacgcgctcg aacccgtggc tggccgaact
     1921 gcagcaggag atgttcgtcg agatcaaccc ggcgaccgcg aacgatcgcg gcatccgcga
     1981 cggcgagaac gtctgggtca cgacgcccac cggcgcgaag atcaccgtca aggcgctggt
     2041 gaccgagcgc gtcgaccgcg agaccgtgtg gctgccgttc cacttctcgg gccgctggca
     2101 gggtgccgac atgctcgcgt actaccccga gggcgcgcac ccggtgatcc gcggcgaggc
     2161 ggtcaacacc gcgaccacct acggctacga cagcgtgacg atgatgcagg aaacgaagac
     2221 caccgtgtgc caggtccagc gggcgacatg acagcccctc gtccgacgaa tacatcggcc
     2281 gatcccccga ggggatgccg agccgccttg ggagcggccc ggcgccgtct cggtggcctg
     2341 accgcctcgc actgaattcc aggagaacac gatggccaga atgaaattca tctgcgacgc
     2401 cgagcgctgc atcgaatgca acggctgcgt caccgcctgc aaggccgagc acaacgtgcc
     2461 ctggggcgtg aaccggcgcc gcgtcgtcac gctgaacgac ggcgtggccg gcgagaagag
     2521 catctcggtc gcctgcatgc actgcagcga tgcgccctgc atggcggtgt gcccggtcga
     2581 ctgcttctac cgcaccgagg agggcgtggt gctgcacgac aaggacgtct gcatcggctg
     2641 cggctactgc tcgtacgcct gtccgttcgg cgcgccgcag tttccgagca acggcgagtt
     2701 cggcctgcgc ggcaagatgg acaagtgcac cttctgcgcc ggcggccccg aggccaacgg
     2761 cagcgaggcc gagaacctga agtacgggcg caaccgcctg tccgagggca agctgccggc
     2821 ctgtgccgag atgtgctcga ccaaggcact gctcggcggc gacggcgacg tggtcgccga
     2881 catcttccgc acccgcgtgc tcaaccgcgg caagggcagc gaggtctggg gctggggcac
     2941 ggcctacggc aagccggtgc aggcgggttc ggccgcatcg cccgccagcg aggtgaagaa
     3001 gtgatcgccc gtgcgctggc cttggtcgcc gcgacggcgc tgacgctgtc gctcgcggcc
     3061 tgcggcgaga agccgcaacg catggacgcc tcggcgcgca aggccgacgc cgcgccctgg
     3121 accgtgtccg acgccgcgaa cccggccttc aaggccccgg gctggaagga cggcgaccag
     3181 gccgcctggg agcagcagat ccgccagcgc aatcaggcgc agaacgacta cgcacgctga
     3241 acgcacgagg agacgacgcg atgcgaagcc cgctctttcg gctgtcctct tttcgatgca
     3301 cgccacgggt gctggcgggc ctgctggccg gcctggccgt cgcgttcgcg ggcgcgcaga
     3361 cggcgcccgg tgcggccgcc tcggcgaccg ccggcgcacc ggcgggcttc gtcgccccgg
     3421 ccgagcccaa ggccgacgag accaacgcgc agcgcgccaa gagccagccc ggcaacaacg
     3481 cgccgttctg gcgctcggtg cacgattccg gcaatcgccc aggcagcgtc aacaacatgg
     3541 tcagcaccga ccggggcgtg ctgatccagg agttcgtgca gtacccgggc tcgcgcgtca
     3601 ccaacgccgg cgaggcctgg cgccaggtgc gcaacggctg gatcattccc tggggcggcg
     3661 cgctggtcgt catcatggtg ctggcgatcg cgctgttcta tttcgcgaag gggccgctgg
     3721 gcgggcacgt gccctacacc ggccgcaaga tcgagcgctt cacgtatttc gaacgcgccg
     3781 cgcactggag caacgcgatc gcgttctgcg tgctcgcgat ctcgggcctg gtgatggcgt
     3841 tcggcaagtt ctggctgctg ccggtgatcg gcgccacctt gttcggctgg ctcacgtatg
     3901 cgctgaagac cgcgcacaac ttcgccgggc cgctgttcgc ggtgtcactg gtgatcgtgt
     3961 tcgtcacctt cctgcgcgac aactttccga gccgcgccga ctggcagtgg ctcaggcgcg
     4021 gcggcggcct gttcggcggc gaggagccgg cgtcggcgcg cttcaatgcc ggcgagaagc
     4081 tgatcttctg gggcggcgtg ttcgtgctcg gcatcgtcgt ggtcgcctcg gggctggtgc
     4141 tcgacaagct cgtgcccggg ctgggctacc tgcgcggcga catgcagatc gcgaacatgg
     4201 tgcatggcgt cgcgaccgtg ctgatgatgg cgatgttcct cggccacatc tacatcggca
     4261 cgatcggcat gaaggacgcc tacggcgcga tgaagaccgg ctacgtcgac gagggctggg
     4321 cccgcgagca ccatgcgctc tggtacgagg acatcaaggc cggcaggatc acggcgcagc
     4381 gcacggcgcc ggccgaaggc cgaccggccg cacaggcctg acacacgaca aggaccgccc
     4441 atcatgcgac tcgccatccc cgcgctgatc gtcggcaccg cactcgcctt gccggcgttc
     4501 gcgaagctgc ccgcaccgag cgacgacgcg aaggccaagg ccgccgcggc cgcggccaag
     4561 accgcgtgga ccgacaaggt cggcgcctac cagctgtgcc gtgcgatgga gcgcacggcc
     4621 gagtactacc gcaagtcgga gaacggcaag caggcgccgg cggcaatcga cacgccgccc
     4681 tgcaccgacc ccggcccgtt cgtcgcgccg ccgccgctgg aagccgcggg cgcgcactcg
     4741 cccgcgacga tggcgacctc gccgccgagc tcgaccgcga ccgccgcgga gcagaagggc
     4801 cagccgaaga agtgagccgc tcccgcatcc ccgcggggga cgggcgccgc atggcggcgt
     4861 ccttggggcg ctatacagtc gccgcatgag catcgcgccc cgcctgacca ccgccaccgc
     4921 agcgctgatg cacgaggtcg atgtgctcga cgaattcggc gcgacgcgcc gcatctcgat
     4981 cccggccgaa cgcgcgctca cggtgttcgt cgacaaccgc gaactggtca cgctgatgac
     5041 gctgggtgcc gcgcccgagc tgctggtgct gggctacctg cgcaaccagc gcctggtcga
     5101 gtcggtcgac gacatcgact cgatcaccgt cgactgggac gtgcaggccg ccgcggtgaa
     5161 gacgcgggcc ggcatcgagc gtttcgacga gaagaccgcg aagcgtgtcg tgacgaccgg
     5221 ctgcggccag ggcaccgtgt tcggcagcct gatggacgac ctcggcgcga tccgcctgcc
     5281 tgctgccgac gagcccgcgg cgcgcatcag ccagcacgcg ttgtacggcc tgctgaacgc
     5341 gatgcggctt caggagagca cctacaagtc ggccggctcg gtgcacggct gtgcactgtt
     5401 caggcaggac gagctgctga tgttcgtcga ggacgtcggc cgccacaacg cgatcgacac
     5461 gatcgccggc tggatgtggc tgaactttgt tgccggcggc gacaagatct tctacacgac
     5521 cgggcgcctg accagcgaga tggtgatcaa gtcggcgcag atgggcgtgc cgatcatcgt
     5581 ctcgcgcagc ggcatcacgc agatgggcca ccagctcgcc caccagctcg gcctcgcgct
     5641 gttcggccgc gcgacgaacc ggcacttcat ctgctacagc ggcttcgatc gcttcgattc
     5701 gcagcccgag ccggcgcgcg gcgtcgcagg caacgtgggc gcgtagcgtg atgccccacc
     5761 tcgcagacgg tcttcacctc gatgaagtac aggccccagg ggcacaggcc gaagcgctcc
     5821 ttgaggggct tgtccttgaa gtccggcagc cggtcggcgt cgtagacggc ccagagcggg
     5881 cccaggccgc cgatgctcag cggcgcgccg tcgagcgcgg tggccacgat catgcggtag
     5941 cgatgcgcgt ccgccaggct cagcgcgacg ttgtagccgt ccagcgcacg cagcgccacc
     6001 atcacgtgcg cgtcggcggc gacgccggcc gcggcgagca ccgagccgag cagggggccg
     6061 ctcagcttgt ggaccttggc gtcgtattcg agcgtgggct ggatcgtcac gctcggcagc
     6121 cgggccagca tcgcggcatc gaactcgcgg gccttgccga actgggttcc gtgcttgacc
     6181 atcagctggt ccagggccgg gtcgagcggg ccccggttgc tgtgggtgat cgcgccggcc
     6241 accgtcagca ggcccgggcc cgtgcgtccc gccgcgggcg ccgcacgtgc gcccaggggc
     6301 aacgcggcac cgagtgccgc tgccgcgaga aaccgtcgtt tgtccattcg atcacctccg
     6361 gtgttgagcg cgaatgatgc accgtccgcg gcgctggcgg ggtgcgtgca cgcccgccgc
     6421 ggcacgaagc ttgcgacgag gttggtgcgg caccgcgacg gtcggcagac agggccgcgg
     6481 cgggcttttt atcagccgaa tgtatcgatc ctctggaccg aaatggtgcg tgcgttccaa
     6541 aagtttcaaa tgcgggtcag ggccgtggcc gaggtgctgg tcggcgcgat gctcggcatg
     6601 atcccggcac ttctgccggc cggcgccttt gtcgcgctgg ccgacatctc gaaggacagc
     6661 gagagcttca tgctggcgat gctgtacgcc ggaagcgtcg gtggctgcgc cgtgggcatc
     6721 gcgttcggca agcgccgcgc cgcgaagcgc catgccgcgc acgactgacc ggcccgtgcg
     6781 catcctgctc gtttccgatc tgcactacac cttgccccaa ctcgattggg tcgtgcgcag
     6841 cgcgccgtcg ttcgatctcg tcgtgctggc cggtgatcag ctggacatca gctcgacggt
     6901 ctcgctcgat gcgcagtccg tcgtgatgct gcgctacctg gcgctgctgc aggcggccgg
     6961 gcgcgtggtg gtgagctcgg gcaaccacga cctgaccggg ccggacgcca atggcgagca
     7021 gtcggccctg tggctgggca aggcgcgcgc cgccggcgtg ccgaccgacg gcgattcgct
     7081 gcgcgtcggc gacacgctga tcacgatctg cccgtggtgg gacggccccc agggccgcct
     7141 cgcgctcgaa gcccagctcg ccgccgatgc ccggcggcga ccggcgcgct ggctctgggt
     7201 gtaccactgg ccgccgctgg ggacgaagac ctgctggacc gggcgccgcg actacggcga
     7261 tccggacctc gtcggttgga tcgcgcagca ccggcccgag gtggtgctga ccgggcacgt
     7321 gcacgagtcg gcgttcaagc cgaccggttg ctgggccgat cgcgtcggcg acacctggat
     7381 cttcaacgcc ggccgtcaga tcggcccggt gccgacccac atcgagctcg acctcgacgc
     7441 cggctcggcc tcatggcggt ccatgctcgg cgtcgaggac atgcgcctgt ccgacacgca
     7501 cgcgccggcg cggacggtct tctgacgcgc gctcgatcag tactcgggtt ccgggtcgcg
     7561 cgccgagccc gcgcgggcgg aagggccctg cagctgcgcg agttcgcgca tcacgtcgga
     7621 caccatcgcc aggcccggcg cgtcgccgta ctggtgcttc aggtcagcca ggtaggtcgt
     7681 gacgaagttg agccgctccg ccaggcccac cgcgaccagc agcgtgatcg cggggtcgcg
     7741 ggtcagcagc gcacggccgt cggccgcgta gcgcagcacg ctgggttcgg tggcttcgac
     7801 ggtcgcgctg tagaccgtgt cgagcagcac cgagacctcg ccgaccagcg cgccggggcg
     7861 gctgacggtg ttgaccaccg aatcgccctt gcgcacctgc aggtcgcccg agacgagcac
     7921 ccagatgccg ccggccgcac cgccctcgcg aacgaccgtc tcgccgcgcg cgaacgccac
     7981 ttcgggcagg ccttccgaga gcgcgagcat gtcgttcatg gcgccatcgt agcccgggat
     8041 tgctgcgccg cacaagtgtg cgcagagaac cgcccgcgaa cgcggcgccc gatgaacggc
     8101 taacgacccg cggcggcgcc gctccactgc tggttgacga acgccgagcc gccgaggtcg
     8161 gcctcggtga acgcgaacac gaaccactgg ttcgggttcg tcctgccgcc cggcgtgacc
     8221 gcgagaaagt cgttgtcgtt gccgatgtac agcgtgtgct tgacgacgcc atcgatcacg
     8281 acatcggcgc cgaaggccat gccttcgagc ttggccggga tctgctcggc ggtgaggccg
     8341 gcggccgtca gcttggcggc gatgtccagg aacagcgact tgccgaccgc atgcgacaac
     8401 agcgcggcct cgccgctcag cgccgacacg tcggtcgcgc ccgcgaggtc gatcttgtag
     8461 atgcgcttga cgaccgcctt gctgtcgtcg cccaggccct tgccgtcgcg ctcgagcacg
     8521 agcagctcat gctcgttcaa cgccagcacc tcgctgctgt tgaaggcctt ggccttgtcg
     8581 gcgaggaagt tgtcgtaggc gtactggtgc acttcgtccg attccaggtc gatcgtcacg
     8641 atgcggttcg cccggccgcc atcgccgccg tcctggatca gcgggctctg cacgaagccg
     8701 aacaggcgct ggccatcggg cgagatcgcc agaccttcca tgcccttgtt ggcgacgcga
     8761 ccgctcgtgt tgcccgagat ctcgacggcg cccatcggcg acttgtgcgc gatggccaga
     8821 ttcgccggca gcttgatcgc gcgcacgcgc gcgccgctgg cgcggtcgaa ctggtagagg
     8881 taggggccgt actcgtcgct gatgtagacg aaccggccgt tcgcggagac gcggatgcct
     8941 tccgggtcca ggcgtgcgtc gcgcgtgtcg gccgacgagg tcgccgggtc gaagttgtcg
     9001 gagcggccgg tgaggtagtg gcgcgacgcc gtgttcgcgg ccggttcggc gcggtagccc
     9061 gcgacgctgg cgccgtagtt cagcggctcg cggctgaaga gcagcgtcgt cgcgacgagc
     9121 gtcggcgtca gcgtgaacgg caggccgctg gcggcatcgg ggcccggctg caggctcagc
     9181 tgcagcgtgt gaaagcgcgg cacgtagccg gtcgtgtcgt tgaccgcggc gttccacgcc
     9241 acggcgttcg ggccgcggtc gggcagcgcg aggaaggtct cgccgcccgc ccaggccagg
     9301 cccgagccga tgccgccgag caggtcgccg cgcacgccgt tctcgagcag cgcgctctgg
     9361 ccggagtggt cgaacacggt gccgggcaat tggccgatgg cgatcaggtc gatcgcatcg
     9421 gcgggggcgg cgaacagggc gcacagcgcc agcgtggcgc tgctgcacag cgtcttcggg
     9481 aaattcatgg tcggtcctcg ggttcacaca gcgatgctgc tcaacgcggc atcgtcgccg
     9541 gccgatgtga ccggcccatg acacctgcgc gtggcgtcgc gggtcagcgc gtgtccgcct
     9601 tcgggtcggg cgccgcggcc cccggcttca cgctgccgtc gggcagcggt accgcgatcg
     9661 gtgccacctc caccgtgg
//
LOCUS       DATMJB010000125         2350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_826948, whole genome shotgun sequence.
ACCESSION   DATMJB010000125 DATMJB010000000
VERSION     DATMJB010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826948"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VNU48_02865"
     CDS             <1..346
                     /locus_tag="VNU48_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWI10247.1"
                     /translation="VHYQKHPEFITLLNTENLHRGKHIAKSLRAREYSSPAVSVLGEV
                     LARGVRQKLFRADVAARDVYLMIASMGYFYLSNRFTLSAFLGEQLDSAEALAHWEVFL
                     VDAVLRTVRRDA"
     gene            424..2271
                     /gene="atzF"
                     /locus_tag="VNU48_02870"
     CDS             424..2271
                     /gene="atzF"
                     /locus_tag="VNU48_02870"
                     /EC_number="3.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004039 - allophanate hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase"
                     /protein_id="HWI10248.1"
                     /translation="MNPSPLLTLPDWQRAYRDGASPRPLLEDLRARLLRDSPPGAWIT
                     RSGPAEIDASVTALEQRAAQHPHRAAALRAMPLFGVPFAVKDNIDVAGLPTTAACPAF
                     ARTPAAHAHAVARLIAAGAVCMGKTNLDQFATGLVGARSPYGRPSSTFAAERISGGSS
                     SGSAVVVSRGDVPFALGTDTAGSGRVPAGFNNIVGLKPTPGRVGNSGVVPACRSIDCV
                     SVLALTVADAAAVLALIEGPDPHDAYSAFRLGAAQPRSALRIGVPIAPVPGADPGYAP
                     CFERALAQVRALGHTAVPIDFEPLLAVAKLLYGGPWVAERHTVVQDLLARDPAALDAT
                     VRAVIEAAHGFSATDTFRAQYLLREAQRDSAALWQQFDLLMVPTAPAHPTHADIDADP
                     LGANALLGTYTNFVNLLGWCALALPAGFTEAGLPFGVTFIAPAAADAALARFGQHWAA
                     SVALPLGATGRAFAADAPEPPATWPASQATLQIAVVGAHLSGLPLNGQLVERGATLRE
                     ATQTAPHYRLFALPDTVPPKPGLMRVGGDASGAAIAVEVWDMPLAEVGAFLALIPPPL
                     GLGSLELADGRWVHGFVCEAHALAGARDVTAYGGWRAFVQVGGNAAPPR"
BASE COUNT          295 a          888 c          837 g          330 t
ORIGIN      
        1 ggtgcactat cagaagcacc ccgagttcat cacgctgctg aacaccgaga acctgcaccg
       61 cggcaagcac atcgcgaagt cgctgcgggc gcgcgaatac tcgtcgccgg cggtcagcgt
      121 gctcggcgag gtgctggcac gcggcgtgcg acagaagctg tttcgtgccg acgtcgcggc
      181 acgcgacgtg tacctgatga tcgcgtcgat gggctacttc tacctgtcga accgcttcac
      241 gctgtcggcc tttctcggcg agcaactcga cagcgccgag gcgctcgcgc actgggaggt
      301 gttcctcgtc gacgccgtgc tgcgaaccgt ccggcgcgac gcgtaggccg caggcgccgc
      361 cgccgcgggc acacttcgtg ctgtgcactt gctggtatac caacgagtgc accattgcat
      421 cgcatgaacc cgagcccgct cctgacgctg ccggactggc agcgcgccta ccgcgatggc
      481 gcttcgccgc gcccgctgct cgaagacctg cgcgcgcggc tgctccggga cagcccgccg
      541 ggggcctgga tcacgcggtc cgggccggcc gagatcgacg ccagcgtcac ggcgctcgag
      601 caacgcgcgg cgcagcaccc gcaccgcgcc gcggccttgc gcgcgatgcc gctgttcggc
      661 gtgccgttcg cggtgaagga caacatcgat gtcgccggtc tgccgacgac cgccgcgtgc
      721 ccggcattcg cgcgcacgcc ggcggcgcac gcgcacgcgg tggcgcggct gatcgcggcc
      781 ggtgcggtct gcatgggcaa gaccaacctc gaccagttcg cgaccggcct ggtcggcgcg
      841 cgctcgccgt acggccgccc gagcagcacc ttcgcggccg agcgcatcag cggcgggtcg
      901 agctccggct cggcggtcgt ggtctcgcgc ggcgacgtgc cgttcgcgct cggcaccgac
      961 accgccgggt cggggcgcgt gccggccggt ttcaacaaca tcgtcgggct gaagccgacg
     1021 ccggggcgtg tcggcaacag cggcgtggtg ccggcctgtc gcagcatcga ttgcgtctcg
     1081 gtgctggcgt tgaccgtcgc cgacgccgcc gcggtgctgg cgctgatcga agggccggac
     1141 ccgcacgacg cctacagcgc gtttcgtctc ggcgcggcgc agcccaggtc ggccttgcgc
     1201 atcggcgttc cgatcgcccc cgtccctggc gcagaccctg gctacgcgcc ctgcttcgaa
     1261 cgggcgctgg cgcaagtgcg cgcgctcggg cacaccgccg tgccgatcga tttcgagccg
     1321 ctgctggccg tcgcgaagct gctgtacggc ggcccgtggg tggccgagcg ccacaccgtg
     1381 gtgcaggacc tgctggcgcg cgacccggcc gcgctcgatg ccaccgtgcg cgccgtgatc
     1441 gaggccgcgc acggcttcag cgcgaccgac accttccgcg cccagtacct gctgcgtgag
     1501 gcccagcgcg acagcgcggc gctgtggcag cagttcgacc tgctgatggt gccgacggcg
     1561 ccggcgcatc cgacgcacgc cgacatcgac gccgacccgc tgggcgcgaa cgcgctgctg
     1621 ggcacctaca cgaacttcgt caacctgctc ggctggtgcg cgctcgcgct gccggccggc
     1681 ttcaccgaag ccgggctgcc gttcggcgtg accttcatcg cgcccgcggc cgcggacgcg
     1741 gccctcgcac gcttcggcca gcactgggcg gccagcgtcg cactgccgct gggcgcgacc
     1801 ggccgggcgt tcgccgcgga cgcccccgag ccgcccgcca cgtggccggc gagccaggcg
     1861 acgctgcaga tcgccgtggt cggcgcgcat ctcagcggcc tgccgctgaa cggccagctc
     1921 gtcgaacgag gcgcgaccct gcgcgaggcc acgcagacgg cgccgcacta ccgcctgttc
     1981 gcgctgcccg acaccgtgcc gcccaagccc ggcctgatgc gggtcggcgg cgacgcgagc
     2041 ggcgcggcca tcgcggtcga ggtctgggac atgccgctgg cagaggtcgg cgcgttcctt
     2101 gcattgatcc cgccgccgct cgggctgggc agcctggagc tggccgacgg ccgctgggtg
     2161 cacggcttcg tctgcgaggc ccacgcgctc gccggcgcgc gcgacgtgac cgcgtacggc
     2221 ggctggcgcg cgttcgtgca ggtcggcggg aacgccgcgc cgccgcgctg agacaccgat
     2281 tcgcctcgtc gtccattcgc caccgggaga tccgcatgag catgaatcac gcagtaccct
     2341 tcgcccgccc
//
LOCUS       DATMJB010000126         3479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_812659, whole genome shotgun sequence.
ACCESSION   DATMJB010000126 DATMJB010000000
VERSION     DATMJB010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812659"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..595
                     /gene="yjgA"
                     /locus_tag="VNU48_02875"
     CDS             59..595
                     /gene="yjgA"
                     /locus_tag="VNU48_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HWI10249.1"
                     /translation="MPMPDDEPTAPAPVFSFDDKPSKTRQKQVSHELQDLGEAAVALP
                     DARLDGLAIDETLLDAIRQFKKTKTFEGRRRQMQYIGKLMRRHDVEPIRQAVTDMQLG
                     RAKDSLALHQAERWRAELIADDDAATRWIAAHPETDVQQLRSLVRAARKDAALVPEKR
                     SGRAFRELFQFIKEHPVE"
     gene            592..1185
                     /gene="mog"
                     /locus_tag="VNU48_02880"
     CDS             592..1185
                     /gene="mog"
                     /locus_tag="VNU48_02880"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HWI10250.1"
                     /translation="MSTLDPVRIGLVSISDRASSGVYADLGIPALQSWLGAAVRNPVH
                     WETRLIADEQAVISAALIELVDTQRCDLVLTTGGTGPAPRDVTPEATLAIADKVMPGF
                     GEQMRQISLVFVPTAILSRQVAVIRGKALVINLPGQRLGDRLRLAGLPTAQPAVHGIF
                     AAVPYCVDLIGGPYIDTEPALCRAFRPKSAQRPAPAA"
     gene            1196..2881
                     /locus_tag="VNU48_02885"
     CDS             1196..2881
                     /locus_tag="VNU48_02885"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HWI10251.1"
                     /translation="MTRPLRHPALTRALFAAALAASLAAARAEDTMVEFVVVRADTLI
                     GLSNSVLVSPTAWREVAKINRLPNPNRILPGQHLRIPTRLLRADAVSATLVSVHGEVQ
                     AGDAPAAPGAAVAEGQSLRTGPDGSAVLALADGSRVRVPPSSLAQVAASRNYGARVPS
                     AAAASADAAAAANAPSHWFAGTLRVLRGSVEVLAAKVLRAKPLEVTTPTAVVGVRGTQ
                     YRVGVDADANGRTHAEVVEGTVHVAATAAAAGADVAAGFGAAVDAGGKIASVAPLLAA
                     PDLSALPERFERPIVRFALPGAGTPLRVQVAADAGFERIVSDQRVAAGAELRIAGLAD
                     AQWQLRARRIDAQGIEGFDATHAFVLKARPEPPAYRTPRASSKQTVGSVEFAWAPNAD
                     APRARLQLASDAAFTRLVQDRDALDGDSLRAELATPGAYYWRLASIKADGDHGPFGDA
                     QRFELRPTPEPAAVAPSADGNALLFRWSGRAEDRQQVEFARDRAFTQIVASAEVSGSE
                     WTLPRPASGGRYYFRYRTVESDGFVSPYGETLQVDVPRDWSGLGLLLPLLLMF"
     gene            complement(2897..>3479)
                     /gene="epsC"
                     /locus_tag="VNU48_02890"
     CDS             complement(2897..>3479)
                     /gene="epsC"
                     /locus_tag="VNU48_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HWI10252.1"
                     /translation="EIHPGATIGRRVFIDHGLGVVIGETAEIGDDCTIYQGVTLGGTS
                     LGRGTKRHPTLGRGVIVGANSQVLGGFTVGDGARVGSNAVVLKEVPPGATAVGNPARV
                     LQKGADAVREAAAAKMGFSAYGITQGDDPLAHAMKGLIDNASGHEHQIALLWQAIEKL
                     SSRSRELPTPDCVPDDAHTTERFDAERFNQLVK"
BASE COUNT          467 a         1298 c         1249 g          465 t
ORIGIN      
        1 tcgcaccaga gtttcgccat cccttcaggc cccgtcccag ctctcggaac caaccccgat
       61 gcccatgccc gacgacgagc ccaccgcacc ggcgcccgtc ttctcgttcg acgacaagcc
      121 gagcaagacg cgccagaaac aggtctcgca cgaactgcag gacctcggcg aggccgcggt
      181 cgcgctgccc gatgcgcgcc tggacggtct cgcgatcgac gagacgctgc tcgacgcgat
      241 ccgccagttc aagaagacca agaccttcga gggccggcgc cgccagatgc agtacatcgg
      301 caagctgatg cggcgccacg atgtcgagcc gatccgccag gccgtgaccg acatgcagct
      361 cggccgcgcc aaggattcgc tcgcgctgca ccaggccgag cgctggcgcg ccgagctgat
      421 cgccgacgac gacgccgcga cgcgctggat cgccgcgcac cccgagaccg acgtgcagca
      481 gctgcgcagc ctggtgcgcg cggcccgcaa ggacgcggcg ctggtgccgg agaagcgcag
      541 cggtcgcgcg ttccgcgaac tgttccagtt catcaaggag caccccgtcg aatgagcacg
      601 ctcgatccgg tgcgcatcgg cctggtctcg atcagcgacc gcgcctccag cggtgtctac
      661 gccgacctgg gcatcccggc gctgcagtcg tggctggggg cggccgtgcg caacccggtc
      721 cactgggaaa cgcgcctgat cgccgacgag caggcggtga tcagcgcggc gctgatcgag
      781 ctcgtcgaca cgcagcgctg cgacctcgtg ctgaccaccg gcggcaccgg gccggcaccg
      841 cgcgacgtga cgcccgaagc cacgctcgcg atcgccgaca aggtcatgcc cggcttcggc
      901 gagcagatgc gccagatcag cctggtcttc gtgccgaccg cgatcctgtc gcgccaggtg
      961 gcggtgatcc gcggcaaggc gctggtgatc aacctgcccg gccagcgtct cggcgatcgc
     1021 cttcggctgg ccggcctgcc gacggcgcag ccggcggtgc acggcatctt cgcggcggtg
     1081 ccgtactgcg tcgacctgat cggcgggccg tacatcgaca ccgagcccgc gctgtgcagg
     1141 gcgttccggc ccaagtcggc gcaacggccg gcgccggccg cctgaggaga gagcgatgac
     1201 ccgcccgctt cgacacccgg ccctgacccg cgctctgttc gccgcggcgc tcgcggcgag
     1261 cctcgcggcg gcgcgagccg aggacacgat ggtcgagttc gtcgtggtgc gcgccgacac
     1321 gctgatcggg ctgtcgaaca gcgtgctggt ctcgcccacg gcatggcgcg aggtcgcgaa
     1381 gatcaaccgg ctgccgaacc cgaaccgcat ccttccggga cagcaccttc gcatcccgac
     1441 acggctgctg cgcgccgatg ccgtcagcgc gacgctggtc agcgtccatg gcgaggtgca
     1501 ggccggcgat gcacccgccg cgcccggtgc cgcggtcgcc gaaggccagt cgctgcggac
     1561 cgggccggac ggatcggcgg tgctggcgct ggccgacggg tcgcgcgtgc gcgtgccgcc
     1621 gtcgagcctg gcgcaggtcg ccgcgagccg caactatggc gcgcgcgtgc ccagcgccgc
     1681 cgcggccagc gccgatgccg cggccgccgc gaacgcgccc agccactggt tcgccggcac
     1741 gctgcgcgtg ctgcgcggtt cggtcgaggt gctcgcggcc aaggtgctgc gcgccaagcc
     1801 gctcgaggtc acgacgccga ccgcggtggt cggcgtgcgc ggcacgcagt accgcgtcgg
     1861 cgtcgatgcc gacgcgaacg gtcgcacgca tgccgaggtc gtcgaaggca cggtgcatgt
     1921 cgccgccacg gcggcggcgg ccggggccga cgtcgccgcc ggtttcggag cggccgtcga
     1981 tgccggcggc aagatcgcga gcgtggcgcc gctgctggcc gcacccgacc tgtcggccct
     2041 gcccgagcgt ttcgagcgcc cgatcgtgcg cttcgcgctg ccgggtgcgg gcacgccgct
     2101 gcgcgtgcag gtggccgccg acgcaggctt cgagcgcatc gtcagcgacc agcgcgtcgc
     2161 cgccggtgcc gagctgcgca tcgccggcct ggccgacgcg cagtggcagc tgcgcgcgcg
     2221 gcgcatcgat gcgcagggca tcgagggctt cgatgcgacg cacgcgttcg tgctgaaggc
     2281 ccggcccgag ccgccggcct accgcacgcc gcgcgccagc tcgaagcaga cggtgggcag
     2341 cgtcgagttc gcgtgggcac cgaacgccga cgcaccgcgg gcgcgactgc agctggccag
     2401 cgacgctgcg ttcacacgcc tcgtgcagga ccgcgacgcg ctcgatggcg acagcctgcg
     2461 cgccgagctg gcgacgccgg gcgcgtacta ctggcgcctg gccagcatca aggccgatgg
     2521 cgatcacggc ccgttcggcg acgcgcagcg cttcgagctg cggcccacgc ccgagccggc
     2581 cgcggtcgcg ccatccgccg acggcaacgc gctgctgttc cgctggagcg gccgcgccga
     2641 ggaccggcag caggtcgagt tcgcgcgcga ccgcgccttc acgcagatcg tcgcgagcgc
     2701 cgaggtgtcg ggctccgagt ggaccctgcc gcgcccggcc agcggcgggc gctactactt
     2761 ccgctaccgc acggtcgagt ccgacggttt cgtcagcccg tacggcgaga cgctgcaggt
     2821 cgacgtgccg cgcgactgga gcggcctggg gctgctgctg ccgctgttgc tgatgttctg
     2881 agccggcgcg ggccgctcac ttcaccagtt ggttgaagcg ctcggcgtcg aaccgctcgg
     2941 tcgtgtgtgc gtcgtcgggc acgcagtcgg gcgtgggcag ttcgcgcgag cgcgacgaga
     3001 gcttctcgat cgcctgccac agcagcgcga tctggtgctc gtggcccgac gcattgtcga
     3061 tcagcccctt catcgcatgc gccagcgggt cgtcgccctg cgtgatgccg taggccgaga
     3121 agcccatctt cgccgcagcg gcctcgcgca ccgcgtcggc ccctttttgc agcacacgcg
     3181 ccgggttgcc gaccgcggtc gcgccgggcg gcacttcctt cagcaccacc gcgttcgagc
     3241 cgacgcgcgc gccgtcgccg accgtgaagc cgcccagcac ctgcgagttg gcgccgacga
     3301 tcacgccgcg gcccagcgtc gggtgccgct tcgtgccgcg gccgagcgag gtgccgccga
     3361 gcgtgacgcc ctggtagatc gtgcagtcgt cgccgatctc ggcggtctcg ccgatcacca
     3421 cgcccaggcc gtggtcgatg aagacgcgcc ggccgatcgt cgcgcccggg tggatctcg
//
LOCUS       DATMJB010000127         2167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_515971, whole genome shotgun sequence.
ACCESSION   DATMJB010000127 DATMJB010000000
VERSION     DATMJB010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515971"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VNU48_02895"
     CDS             complement(<1..261)
                     /locus_tag="VNU48_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWI10253.1"
                     /translation="MARDLGPKCRLCRREGIKLFLKGERCLTEKCAVERRSYPPGEHG
                     RGRIKQSQYLLQLREKQKARRYYGLLEKQFRTTYEKATRGLGA"
     gene            complement(261..665)
                     /gene="rpsK"
                     /locus_tag="VNU48_02900"
     CDS             complement(261..665)
                     /gene="rpsK"
                     /locus_tag="VNU48_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015237686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWI10254.1"
                     /translation="MAPPRRQAGRGRTRRRVRKNIAVGQAHIKTSFNNTIVTLTDKDG
                     AVIVWESAGSAGFKGSRKSTPFAAQVTADACARKGMEHGLQKVEVFVKGPGSGRETAI
                     RSLQAAGLEILGVKDVTPQAHNGVRQRKRRRV"
     gene            complement(665..1042)
                     /gene="rpsM"
                     /locus_tag="VNU48_02905"
     CDS             complement(665..1042)
                     /gene="rpsM"
                     /locus_tag="VNU48_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWI10255.1"
                     /translation="MARIAGINIPPHKHAEIGLTSIYGIGRTTAQKICSNSGVPFDRK
                     VKDLTDADLEKIREEIGRLTIEGDLRREMSINIKRLMDLGCYRGFRHRRGLPVRGQRT
                     KTNARTRKGPKKTVGRGKRAAVK"
     gene            complement(1070..1183)
                     /gene="rpmJ"
                     /locus_tag="VNU48_02910"
     CDS             complement(1070..1183)
                     /gene="rpmJ"
                     /locus_tag="VNU48_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HWI10256.1"
                     /translation="MKVAASVKKMCRNCKVIRRKGVVRVICSDPRHKQRQG"
     gene            complement(1225..1443)
                     /gene="infA"
                     /locus_tag="VNU48_02915"
     CDS             complement(1225..1443)
                     /gene="infA"
                     /locus_tag="VNU48_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HWI10257.1"
                     /translation="MAKDDVIQMQGEILENLPNATFRVKLENGHVVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLSRARIVFRAK"
     gene            complement(1452..>2167)
                     /locus_tag="VNU48_02920"
     CDS             complement(1452..>2167)
                     /locus_tag="VNU48_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWI10258.1"
                     /translation="QFATLVGQGSMSIVAALFIAALVVAVTFAVVFVERGQRKILVNY
                     AKRQVGNKVYGGQSSHLPLKLNMSGVIPPIFASSIILLPATAVGWFATGESFRWLKDV
                     ADLLRPGQPIYVMLYAAAIVFFCFFYTALVFNSRETADNLKKSGAFIPGIRPGDQTAR
                     HIDRILSRLTLAGAIYITLVCLLPEFLVLKYNVPFNFGGTSLLIIVVVTMDFWAQVQS
                     YVMSQQYESLLKKANFKAS"
BASE COUNT          354 a          700 c          690 g          423 t
ORIGIN      
        1 agcgccgagg ccgcgcgtcg ccttctcgta ggtcgtgcgg aactgcttct cgaggagccc
       61 gtagtagcgg cgcgccttct gcttctctcg cagctggagc agatactgac tctgcttgat
      121 gcgcccccgc ccgtgctcgc ccggcggata gctgcggcgc tcgaccgcgc acttctccgt
      181 gaggcagcgc tcgcctttca ggaagagctt gatgccctcg cggcggcaga gccggcactt
      241 ggggccgagg tcgcgggcca tcagacgcgc ctccgcttgc gctggcggac gccgttgtgc
      301 gcctgcggcg taacgtcctt gacgccgagg atctcgaggc cggcggcctg caaggagcgg
      361 atcgccgtct cgcgccccga gcccgggccc ttgacgaaga cctcgacctt ctggaggccg
      421 tgctccatgc ccttccgggc gcaggcgtca gccgtcacct gcgcagcgaa cggcgtcgac
      481 ttgcgcgacc ccttgaaccc ggccgagccg gcggattccc acacgatcac cgcgccgtcc
      541 ttgtccgtga gcgtgacgat cgtgttgttg aacgacgtct tgatgtgcgc ctggcccacg
      601 gcgatgttct tgcggacgcg gcggcgcgtc cggccacgac cggcttgtcg gcgaggaggc
      661 gccattactt gaccgccgcc ctcttgccgc gcccgacggt cttcttcggg cccttgcggg
      721 tgcgggcgtt cgtcttcgtg cgctggccgc gcacgggcag gccgcgacga tggcggaaac
      781 cacggtagca gccgagatcc atcaggcgct tgatgttgat cgacatctcg cgacgcaggt
      841 cgccttcgat cgtcaggcgg ccgatttctt cgcggatctt ctccaggtcg gcgtcggtca
      901 ggtccttgac cttgcgatcg aacggcacac cgctgttcga gcagatcttc tgggccgtcg
      961 tgcgaccgat cccatagatc gaggtcagcc cgatctcggc gtgcttgtgg ggcggaatgt
     1021 tgatgccagc aatacgtgcc atgtttgttt ctctcttgac ccgagggcct tagccttgac
     1081 gctgcttgtg gcgcggatcg ctgcagatca cgcgcacgac gcccttgcgc cgaatcacct
     1141 tgcaattgcg gcacatcttc ttgaccgatg ctgcgacttt catgctcttc tctcctcggc
     1201 cccgtcagag ggccttcaaa atttttactt cgcccggaac acgatccgag cacggctcag
     1261 atcgtagggc gtcaactcga cagtgacctt gtcgcccggc aggatgcgga tgtagtgcat
     1321 gcgcatcttg ccggagatgt ggccgagcac gacgtgcccg ttttccagct tcactcggaa
     1381 cgttgcattg ggcagattct cgagaatctc gccctgcatc tgaatgacat cgtctttggc
     1441 catgtactgt ttcagctcgc tttgaagttg gccttcttga gcagggactc gtactgctgg
     1501 ctcatcacat aactctgcac ctgggcccag aaatccatcg tcacgacgac gatgatcagc
     1561 agcgaggtgc cgccgaaatt gaagggcacg ttgtacttca gcaccaggaa ctcgggcagc
     1621 aggcacacca gcgtgatgta gatcgcgccg gccagcgtga ggcgcgacag gatgcggtcg
     1681 atgtggcgtg ccgtctggtc gcccgggcgg atgcccggaa tgaacgcacc gctcttcttc
     1741 aggttgtcgg cggtctcgcg gctgttgaac acgagcgcgg tgtagaagaa gcagaagaac
     1801 acgatcgccg cggcgtacag catcacgtag atcggctggc ccgggcgcag cagatcggcc
     1861 acgtccttga gccaacggaa gctctcgccg gtcgcgaacc agcccaccgc ggtggccggc
     1921 agcaggatga tgctcgaggc aaagatcggc gggatcacgc ccgacatgtt cagcttcagc
     1981 ggcaggtgcg aggactgacc accgtagacc ttgttgccga cctggcgctt cgcgtagttc
     2041 acgagaatct tgcgctggcc gcgttcgacg aacacgaccg caaaggtcac cgcgacgacc
     2101 agtgcggcga tgaacagcgc cgcgacgatg ctcatcgagc cctggccgac gagggtggcg
     2161 aactgtc
//
LOCUS       DATMJB010000128         2394 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_634308, whole genome shotgun sequence.
ACCESSION   DATMJB010000128 DATMJB010000000
VERSION     DATMJB010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634308"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VNU48_02925"
     CDS             <1..230
                     /locus_tag="VNU48_02925"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10259.1"
                     /translation="DGERTEQRMSLYLKDLGSQEAHDALGKPFVERFVYEVEQDLKVL
                     DFKKHVLQPKLCRGDGPIYPYREYAARYLPN"
     gene            290..1027
                     /locus_tag="VNU48_02930"
     CDS             290..1027
                     /locus_tag="VNU48_02930"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI10260.1"
                     /translation="MDGRVVIITGAASGLGEASSRLFAEHGARVVMADINAERGRAIA
                     REIGARFVETDVSHSVAVDRLVSETVEREGRLDVMFNNAGVATPFGPLIDTDDKVFDL
                     VSRVNFSGMFYGIRAAGRVMREQRRGVIISTASTGGIVVAPGMTAYCATKAAVIALTK
                     GCALELAQFGVRVNALCPGAMFTGMTAGISDEQRRWMNNISPMGWAAQPIEMAKGALY
                     LASDDAAYVSGHELVVDGASIAGFRYQ"
     gene            complement(976..1242)
                     /locus_tag="VNU48_02935"
     CDS             complement(976..1242)
                     /locus_tag="VNU48_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10261.1"
                     /translation="MDKAIAASTGGVGTRAMSNIATASVTLCATVNAVMVFRSVPRPA
                     INVVQRDVEDRIHDVVLRGEQRPMAASIHWYRNPAIEAPSTTSS"
     gene            complement(1280..2263)
                     /locus_tag="VNU48_02940"
     CDS             complement(1280..2263)
                     /locus_tag="VNU48_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI10262.1"
                     /translation="MSTRHLVDPELLPLLEVFPSFEFSLEQLPLIREQAKAMGAASAP
                     ISRRGVTVCERHVPGPADGPAVRVLIYAPAAAATARPALLHIHGGGYVVGAPEMDDGR
                     NKALAAELDCVVVSVDYRLPPEFPFPAPIEDCHAVLTWLFRQASELGVDPKRIGVYGE
                     SAGGGLAAALALLARDRESVPIAFQLLIYPMLDDRTCVVDPHPLVGEFIWPRPANAFG
                     WRCLLGQAPGAKGVSAYASAARASSLAGLPPTFIGVGALDLFLDENIDYARRLTQAGV
                     PTELHVYPGAYHGFEMLAPEARVSRQFERDLRHALRKALVAAPEASSGEPA"
BASE COUNT          443 a          768 c          794 g          389 t
ORIGIN      
        1 tggacggcga gcgcaccgag cagcgcatga gcctgtacct gaaggatctc gggtcacagg
       61 aagcgcatga cgcgttgggc aagccgttcg tcgagcgctt cgtctacgaa gtcgaacagg
      121 acctgaaagt gctggacttc aagaagcacg tgctgcagcc caagctgtgc cgtggcgacg
      181 ggccgatcta cccgtatcgc gagtacgcgg ccaggtacct gccgaattga agatcgtcgc
      241 tcccatcgca accagatcac agactcgagg agaccccatg gagaagagat tggacggacg
      301 cgtcgtcatc atcactggcg cggcgtcggg gctcggcgag gcatcgtcga ggctgtttgc
      361 agaacacggt gctcgtgtcg tgatggcgga catcaacgct gaacgcggcc gcgcgattgc
      421 gcgcgagatc ggcgcgcgct tcgttgaaac cgacgtcagc cattcggtag ctgtcgatcg
      481 gctggtgagc gaaaccgttg agcgcgaggg ccggcttgac gtgatgttca acaacgccgg
      541 ggtggcgacg ccgttcggcc cgctgatcga caccgatgac aaggtgttcg atctcgtgtc
      601 gcgagtgaac ttcagcggca tgttctacgg cattcgcgcc gccggtcgcg tcatgcgcga
      661 acaacggcgc ggcgtcatca tcagcaccgc atcgaccggc ggcattgtcg tggcgccggg
      721 aatgaccgcc tactgcgcga ccaaggcagc ggtgatcgca ctgaccaagg gctgcgcgct
      781 cgagttggcg cagttcggcg tacgggtcaa tgcgctgtgc ccgggcgcaa tgttcacagg
      841 catgaccgcc ggcatcagcg acgagcagcg gcgttggatg aacaacatct cgcccatggg
      901 ctgggcggcg cagcccatcg aaatggccaa gggtgcgctt tatctggcat ctgacgacgc
      961 ggcctacgtc agcggtcacg aactagtggt cgacggcgcc tcgatcgccg ggttccgata
     1021 ccaatgaatg gaagcggcca tcggccgttg ctcgccgcga aggaccacgt catgaattcg
     1081 atcttcgaca tcccgctgca ccacattgat ggccggtcgc ggcacgctcc tgaagaccat
     1141 cacggcgttc acggtcgcac acagtgtcac gctggccgtc gcgatgttcg acatcgcgcg
     1201 cgtgccgacg ccaccggtcg aagcggcgat cgccttgtcc atcctgttcc tcgcaggcaa
     1261 cgaacgactg cgtgggcgct caagccggct cgccgctgct tgcctcgggc gccgcaacca
     1321 gggccttgcg caacgcatgg cgcaggtcgc gctcgaactg tcgggagacg cgggcttcgg
     1381 gcgcgagcat ctcgaagccg tgataggcac ccgggtacac gtggagttcg gtcgggacgc
     1441 cggcttgtgt caatcggcgc gcatagtcga tgttttcgtc gagaaacaga tccagcgcgc
     1501 ccacgccaat aaaggtgggc ggcaggccgg cgagcgagct cgcgcgcgcg gccgaggcgt
     1561 aagctgagac gcccttcgcg ccgggtgcct gacccagcag gcaacgccaa ccgaaggcat
     1621 tggcgggccg gggccagatg aactcaccga ccaagggatg cgggtcgacg acgcaagttc
     1681 gatcgtcgag catcgggtag atcaacaatt gaaaggcgat gggtaccgac tcccggtcgc
     1741 gcgccagcaa ggcgagtgca gccgccaggc ctcctccagc gctctcgcca tacaccccga
     1801 tgcgcttcgg gtcgacgccc agttcagacg cctgccggaa caaccacgtc agcacggcgt
     1861 gacagtcctc gatcggtgca ggaaacggaa actccggcgg cagccggtag tccaccgaca
     1921 cgaccacgca gtccagctcg gccgccaagg ccttgttgcg tccatcgtcc atctccggcg
     1981 cgccgacgac gtaaccgccc ccgtggatgt gcagcagcgc aggccgggct gtcgcggcgg
     2041 cggcaggggc gtatatcagc acccggacgg ccgggccatc cgccgggccc gggacgtgac
     2101 gctcgcacac cgtcacgccc cgccgcgaga tgggggcgct ggccgcgccc atggccttgg
     2161 cttgctcccg aatcagcggc aactgctcga gcgaaaactc gaaactgggg aacacctcca
     2221 gcaatggcaa gagttcgggg tcgacaagat gccgcgtgga catggctcgc tccttcctgg
     2281 gtgggcttga aagggtgtgg ggcgagagtg attttccagg tccactggcg ttctctcccg
     2341 gattcgttca cccttcccaa tttcgcctgg accggcacgg attgcatcgc ccgt
//
LOCUS       DATMJB010000129         4654 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_753094, whole genome shotgun sequence.
ACCESSION   DATMJB010000129 DATMJB010000000
VERSION     DATMJB010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753094"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /gene="lptD"
                     /locus_tag="VNU48_02945"
     CDS             <1..719
                     /gene="lptD"
                     /locus_tag="VNU48_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HWI10263.1"
                     /translation="GVDRVSDAHQVTAGVTTRFLDPDSGAERLRLGMAQRYLLRDQLV
                     TPDGQPLTRRFSDLLLLASTTVIPSWTLDSSVQYSPDSNRVSRSVVSARYSPGPFRTI
                     STTYRLTRGLSEQVELGWQWPLYGPTPNEPRPAGAGSGCQGSWYGVGRVNYSTLERRL
                     VDAIVGVEYDAGCWIGRIVATRQSTGLSEATTRLLFQLELVGLSRLGSNPLQVLKDNI
                     PGYKLLREGRAVAPASSAYD"
     gene            712..2058
                     /locus_tag="VNU48_02950"
     CDS             712..2058
                     /locus_tag="VNU48_02950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWI10264.1"
                     /translation="MTESLFFRRSWVGALGRISCLFALGLAAPLAPAQTRASAAPNTA
                     DFIIAVVNQELVTNGELQGRIARIREDAARSNTALPAPAELRKKVLDALIDERVQVTN
                     ARETGPKIDEAELDRAVGNVAVQNQMTMAQLRARLQQQGLAYSAFRNNVKDQLLVERV
                     REREVNQRIRVTDAEIDALLEQRRASSGKAVQLDIAQILVTVPEGASAEQIAQRRARA
                     EGALARVKGGEDFAAVAREVSEDANKLQGGAIGLRLADRLPDVFVDTVRNLKPGEVSP
                     TLLKSGAGFHVLKLLTRTEGSAFSVQQTRARHILLRVSAQLSAEAAQRRLADFRRQIL
                     AGTKSFEAIARENSEDGSAAIGGDLGWTSPGTFVPEFEETMNALPINGLSDPVVSRFG
                     VHLIQVLERREVMLDAKQQREQARNVLREQKFERAYLDWVRDLRGLAYIEMREAPQ"
     gene            2064..2849
                     /gene="rsmA"
                     /locus_tag="VNU48_02955"
     CDS             2064..2849
                     /gene="rsmA"
                     /locus_tag="VNU48_02955"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWI10265.1"
                     /translation="MKHIARKRFGQHFLTDGAVIDAIVDAIDPQAGDALVEIGPGLGA
                     MTDPLVARGAKLTVIELDRDLAERLRRRAELDVIEADVLQVDFALMAQRRGQRLRVVG
                     NLPYNISTPILFHLLGAVEHVVDQHFMLQKEVVDRMAAAPGSKDYGRLSVMLQWRYAI
                     ESVLDVPPTAFDPPPRVESAVVRMQPLPPLPTVDAALLEQLVRVAFSQRRKLLRNTLG
                     RWLDERRHAGGFDLQRRAEEVPVGEYLALAAALGPEQTQLTPR"
     gene            2846..2998
                     /locus_tag="VNU48_02960"
     CDS             2846..2998
                     /locus_tag="VNU48_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10266.1"
                     /translation="MSHIAVSKALLISGMFMPKLAFAALFAAGLTGLSATLGVTSVAR
                     SQDARS"
     gene            complement(3006..3401)
                     /locus_tag="VNU48_02965"
     CDS             complement(3006..3401)
                     /locus_tag="VNU48_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HWI10267.1"
                     /translation="MLLQTVRPNIVQSIRAFRVEDLMQAAQGAEQHFLYANLTTAQSK
                     QDVLEAIAQAFMFPAHFGKNLDALYDCMTDLVHKSGAQPGFVVVLEQLPDNPRFDREA
                     REQLLDVFRDAADFWAERKIPFRCFYSFQ"
     gene            complement(3458..3781)
                     /locus_tag="VNU48_02970"
     CDS             complement(3458..3781)
                     /locus_tag="VNU48_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HWI10268.1"
                     /translation="MAGTVQARGSLPETVALAQLPAEVQATHRLIRAGGPFPHHKDGS
                     VFGNRERALPARPRGYYHEYTVDTPGARDRGARRIVCGGRPPTQPDACFYTADHYASF
                     RQIAP"
     gene            complement(4104..>4654)
                     /locus_tag="VNU48_02975"
     CDS             complement(4104..>4654)
                     /locus_tag="VNU48_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HWI10269.1"
                     /translation="AKTYACMNGLILPGRQVMLVDTHVNYDPSAEQLAEITIMAAEEM
                     MRFGLQPKAALLSHSNFGSSNQPSAVKMRNTLALLQQRSPWLEVDGEMHGDTALDAAY
                     RKTIMPNSALNGEANLLVLPNIDAANISYNLLKTAAGGGIAIGPVLLGAARPVHILTP
                     SATVRRIVNMTALTVADANAAR"
BASE COUNT          759 a         1572 c         1589 g          734 t
ORIGIN      
        1 cgggcgtgga ccgggtctcc gacgcgcacc aggtcaccgc cggcgtgacc acccgcttcc
       61 tcgaccccga cagcggtgcc gaacggttgc gcctgggcat ggcgcagcgc tacctgctgc
      121 gcgaccagct cgtcacgccc gacggccagc cgctgacgcg gcgcttctcg gacctgctgc
      181 tgctggcgtc gacgaccgtg attccgtcgt ggacgctcga ctcgtcggtg cagtacagcc
      241 ccgacagcaa ccgggtgtcg cgctcggtcg tcagcgcacg ctattcgccg gggccgttcc
      301 gcacgatcag caccacctac cggctgacac gcggcctgag cgaacaggtc gagctcggct
      361 ggcagtggcc gctgtacggc cccacgccga acgagccgcg ccccgccggc gcgggcagtg
      421 gctgccaggg cagctggtac ggtgtgggtc gcgtcaacta cagcacgctg gagcgccggc
      481 tcgtcgatgc gatcgtcggc gtcgagtacg acgccggctg ctggatcggc cgcatcgtcg
      541 cgacgcgcca gtccaccggc ctgagcgagg ccaccacccg attgctgttc cagctggaac
      601 tcgtcggcct gtccagactc ggaagcaacc cgctccaggt cttgaaggac aatatccccg
      661 gatacaagct gctgcgcgaa ggacgtgcag tggccccagc gtcgagtgcc tatgactgaa
      721 tcgttgttct tccgccgctc ttgggtcggc gctctcggcc gcatctcgtg cctgttcgcg
      781 ctggggctgg ccgcgccgct cgcgcccgcg cagacgcgcg cgtcggccgc ccccaacacc
      841 gccgacttca tcatcgcggt ggtcaaccag gaactcgtca cgaacggcga attgcagggg
      901 cggatcgcgc gcatccgcga agacgcggcg cgcagcaaca cggcactgcc ggcgccggcc
      961 gaattgcgca agaaggtgct ggacgcgctg atcgacgagc gcgtgcaggt caccaacgcg
     1021 cgcgagaccg ggccgaagat cgacgaggcc gaactcgacc gcgcggtcgg caacgtcgcg
     1081 gtgcagaacc agatgacgat ggcgcagctg cgcgcgcggc tgcagcagca gggcctggcg
     1141 tacagcgcgt ttcgcaacaa cgtgaaggac cagctgctgg tcgagcgggt gcgcgagcgc
     1201 gaggtcaacc agcgcatccg cgtcaccgac gccgagatcg acgcgctgct cgagcagcgc
     1261 cgcgcatcga gcggcaaggc ggtgcagctc gacatcgcgc agatcctcgt caccgtgccc
     1321 gagggcgcga gcgccgagca gatcgcgcag cgccgcgccc gcgccgaagg cgcactggcg
     1381 cgcgtcaagg gcggcgagga tttcgcggcc gtggcacgcg aagtgtccga ggacgccaac
     1441 aagctgcaag gcggggcgat cgggctgcgc ctggccgatc gcctgccgga cgtgttcgtc
     1501 gacaccgtgc gcaacctcaa acccggcgag gtctcgccga cgctgctcaa gagcggcgcc
     1561 ggcttccacg tgctgaagct gctgacgcgc accgaaggct ctgcgttctc ggtgcagcag
     1621 acgcgcgcac gccacatcct gttgcgcgtc tcggcccagc tgtccgccga agcggcccag
     1681 cggcggctgg cggacttcag gcgccagatc ctggccggca ccaagtcgtt cgaggcgatc
     1741 gcgcgcgaga actccgaaga cgggagcgcg gcgatcggcg gcgatctcgg ctggacctcg
     1801 cccggcacct tcgtgcccga gttcgaggag acgatgaacg cgttgccgat caacggcctg
     1861 tcggacccgg tggtgtcgcg cttcggcgtg cacctgattc aggtgctcga gcgccgcgag
     1921 gtcatgctcg acgccaagca gcagcgcgaa caggcgcgca acgtgctgcg cgagcagaag
     1981 ttcgaacgcg cctacctcga ctgggtgcgt gacctgcgcg gcctggccta catcgagatg
     2041 cgcgaggcgc cgcagtaggc gtcgtgaagc acatcgcgcg caagcgcttc ggtcagcatt
     2101 tcctgaccga tggcgcggtc atcgacgcga tcgtcgacgc gatcgacccg caagccggcg
     2161 atgcgctggt cgagatcggc cccgggctgg gggcgatgac cgacccgctg gtggcgcgcg
     2221 gcgcgaagct caccgtgatc gagctcgatc gcgacctcgc cgagcggctg cgccgccgcg
     2281 ccgagctcga cgtgatcgag gccgatgtgc tgcaggtcga tttcgcgctg atggcgcagc
     2341 gccgtggcca gcggctgcgg gtcgtcggca acctgccgta caacatctcg acgccgatcc
     2401 tgttccacct gctcggcgcc gtcgagcacg tcgtcgacca gcacttcatg ctgcagaagg
     2461 aagtggtcga ccgcatggcc gcggcgcccg gcagcaagga ctacggtcgt ctcagcgtga
     2521 tgctgcagtg gcgctacgcg atcgaatcgg tgctcgacgt gccgcccacg gccttcgacc
     2581 cgccgccgcg cgtcgagtcg gcggtcgtgc gcatgcagcc gctgccgccg ctgccgacgg
     2641 tcgatgcggc gctgctcgaa caactggtgc gcgtcgcgtt ctcgcagcgc cgcaagctgc
     2701 tgcgcaacac gctcggtcgc tggctcgacg agcgccgcca cgccggtggg ttcgacctgc
     2761 aacggcgcgc cgaggaagtg ccggtcggcg aatacctggc actggcggcc gcgctcgggc
     2821 cggaacagac gcagctcacg ccgcgttgag ccacatcgcc gtgtcgaaag cgctgctcat
     2881 cagcggcatg ttcatgccga agctcgcgtt cgccgcgctc ttcgcggccg gcttgaccgg
     2941 cttgtccgca acgctggggg ttacttcggt agcccgctcc caagacgcga ggtcttgaga
     3001 gcgggctact gaaaagaata gaagcacctg aaggggattt tccgttccgc ccagaagtcg
     3061 gcggcgtctc ggaacacgtc gagcaactgc tcgcgcgcct cgcgatcgaa gcgcgggttg
     3121 tcgggcaact gctcgagcac gaccacgaac ccgggttgcg cgcctgactt gtggaccaga
     3181 tcggtcatgc agtcgtacag cgcgtccagg ttcttgccga aatgcgccgg gaacatgaaa
     3241 gcctgcgcga ttgcctcgag aacgtcctgc ttggactggg ccgtcgtcag attcgcgtac
     3301 aggaaatgct gctcggcgcc ttgcgccgcc tgcatcagat cctccacacg aaatgcgcgg
     3361 atcgattgaa caatgttcgg acggactgtc tgcaacagca tggtcacgtt cctcctcgtg
     3421 tcaaaaaaag atctcgaacc aaaactcgtg tgcccggtca cggcgcgatc tgtcggaacg
     3481 acgcgtagtg atcggcggta tagaaacagg cgtccggttg cgtgggcggc ctgccaccgc
     3541 agacgatgcg ccgcgcgccg cgatcgcgcg cgccgggcgt gtcgaccgtg tactcgtggt
     3601 agtagccgcg cggccgggcc ggcagcgcac gttcgcggtt gccgaatacg ctgccgtcct
     3661 tgtggtgcgg gaacgggcca ccggcgcgga tcaggcggtg cgtcgcctgc acttcggcag
     3721 gcaactgagc cagtgcgacg gtttccggca gcgaacctcg ggcctgaacc gtaccggcca
     3781 tcaagaccgc gcctgccaag gtgacaacca gccccgtctt gtgcagagct tgccaccacg
     3841 gtggccgacc ggagaaaaac acggggaaat ccctgaattc agaagcgccg atgttaccga
     3901 atcgggccgg aaatctcaag cctctgcctc aaaatggggc aaatgagcca gtttgtttgt
     3961 gtgcacccac ttgtcagatg tgggtcggaa acattgcgac cgacgcgctt gccatcgtcc
     4021 gaaaacaagc tgcgcgcgcg atggcgacgg ccgtggatat ccttcgggtt tccccggata
     4081 tcgcccgccg gcgctcgacg agatcagcgt gccgcgttgg cgtcggccac ggtcaaggcg
     4141 gtcatgttga cgatgcggcg cacggtcgcc gagggcgtca ggatgtgcac cggccgggcc
     4201 gcgccgagca gcaccgggcc gatcgcgatg ccgccgccgg cggctgtctt cagcaggttg
     4261 tagctgatgt tcgcggcgtc gatgttcggc agcacgagca ggttggcttc gccgttcagc
     4321 gcactgttgg gcatgatggt cttgcggtag gcggcgtcga gcgcggtgtc gccgtgcatc
     4381 tcgccatcca cttcgagcca cggcgagcgc tgctgcagca gcgccagcgt gttgcgcatc
     4441 ttgaccgccg agggctggtt gctcgagccg aaattgctgt gcgacaacag cgccgccttc
     4501 ggctgcagcc cgaatcgcat catctcctcg gccgccatga tcgtgatctc ggcgagctgc
     4561 tccgcgctcg ggtcgtagtt gacatgggtg tcgaccagca tcacctggcg ccccggcagg
     4621 atcagcccgt tcatgcaggc gtaggtcttg gcgc
//
LOCUS       DATMJB010000130         2297 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_397154, whole genome shotgun sequence.
ACCESSION   DATMJB010000130 DATMJB010000000
VERSION     DATMJB010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397154"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..487)
                     /gene="hpxZ"
                     /locus_tag="VNU48_02980"
     CDS             complement(89..487)
                     /gene="hpxZ"
                     /locus_tag="VNU48_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalurate catabolism protein HpxZ"
                     /protein_id="HWI10270.1"
                     /translation="MEVDLPDVLAEVTEQFARYEKALVSNDVAVLDELFRPDSRTLRY
                     GAGENLYGYEAIMAFRAARSPVGLGRRTDKTVITTYGRDTAVASTLFYREALPGRVGR
                     QMQTWVRFPEGWRIVAAHVSVIDEPRDQKA"
     gene            complement(488..1885)
                     /locus_tag="VNU48_02985"
     CDS             complement(488..1885)
                     /locus_tag="VNU48_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtzE family amidohydrolase"
                     /protein_id="HWI10271.1"
                     /translation="MSANLDGQSASEIAQAISGKKLSALGVTEAALARIAKHNPVLNA
                     FTDVTADRARARARTVDAAIAAGKNAGPLAGVPFAVKNLFDVEDLPTRAGSRINRDLA
                     ASKRDATLIERMEAAGAVLVGALNMGEYAYDFTGENAHDGPSRNPHDVTRMTGGSSGG
                     SGAAVGGGLVPIALGSDTNGSIRVPSSFCGIFGLKPTYGRLSRARSFPFVASFDHLGP
                     FARNVGDLALAYDAMQGPDADDAACAPRAAEPVTALLAQDIGGLRVAIAGGYFQKNVF
                     PEAVEAVARVAKGLGATRVVELPEAARARAAAYVISTAEGASLHLDRLRQRAHDFDPA
                     VRDRLIAGAMVPAVMVDRAQKFRRWYRARMMELFKSVDVVIAPATPCTAPQLGQATFM
                     LDGVELPVRANIGIHTQPISFIGLPVVAVPVPLQPMPIGVQIIAAPWREDIALRVAHA
                     LERDGVVSAPEPRGL"
     gene            complement(1893..2075)
                     /locus_tag="VNU48_02990"
     CDS             complement(1893..2075)
                     /locus_tag="VNU48_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4089 domain-containing protein"
                     /protein_id="HWI10272.1"
                     /translation="MAADPLDDYIDAVSKALALPVEDAWRPAVKANLEVSLRLARLVD
                     EFALPDETEPASVFTA"
     gene            complement(2108..>2297)
                     /locus_tag="VNU48_02995"
     CDS             complement(2108..>2297)
                     /locus_tag="VNU48_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI10273.1"
                     /translation="GMSYLFVSHDLNVVRLLCDRVIVMRSGRIVEQGLSERVLGDPQD
                     AYTKELLTAIPHPPLPAH"
BASE COUNT          417 a          789 c          733 g          358 t
ORIGIN      
        1 tagcgcgaag cgcgatcgac gcgccgaacc ggcgtgtcgg tctcgccggc cccgcgctgc
       61 gtcgggctgc tcggaagatc gtccaaactc atgccttttg atctcgcggt tcgtcgatga
      121 cgctgacatg ggcggcgacg attctccaac cctcggggaa acgcacccag gtctgcattt
      181 gccgccccac ccggccgggc aaggcctccc ggtagaacag cgtggaggcc acggcggtgt
      241 cacggccata ggtcgtgatc accgtcttgt cggtgcgacg gccgaggcca accggcgaac
      301 gcgcggcgcg aaacgccatg atcgcctcat agccataaag attttcaccg gccccgtagc
      361 gcagcgtgcg ggaatccggc cgaaacaact catcgagcac ggcgacgtcg ttggagacca
      421 gcgccttttc gtaacgcgcg aactgttcgg tcacctcggc gagcacatcc ggaagatcga
      481 cttccattca caatcctctc ggctcgggcg cggaaaccac gccgtcgcgc tccagcgcgt
      541 gggcgacgcg caacgcgata tcttcccgcc agggtgcggc gatgatctgc acgccgatcg
      601 gcatcggctg cagcggcacc ggcaccgcga ccaccggcaa cccgatgaag gaaatcggct
      661 gggtatggat accgatattg gcgcgcaccg gcagttcgac gccatcgagc atgaaggtcg
      721 cctgccccag ttggggggcc gtgcaaggcg tcgccggcgc gatgaccacg tcgaccgact
      781 tgaacagctc catcatccgg gcgcgatacc agcggcggaa tttctgcgcg cgatcgacca
      841 tgacagccgg aaccatcgcc cccgcaatca accggtcgcg caccgcggga tcgaaatcgt
      901 gcgctcgctg gcgcaggcgg tcgagatgca gcgatgcgcc ctcggcggtg gagatgacgt
      961 aggcggcggc gcgggcgcgc gcggcttcgg ggagttcgac gacccgcgtc gcgccgaggc
     1021 cctttgccac gcgcgccacc gcctcgacgg cttcgggaaa aacgtttttc tggaaatatc
     1081 cgccggcaat cgcaacccga aggccgccga tgtcctgcgc cagcagcgcc gttaccggct
     1141 cagccgcccg cggggcgcag gcggcatcgt cggcatccgg tccctgcatc gcgtcatagg
     1201 ccagcgcgag atcgccgacg ttgcgcgcga acgggccgag atgatcgaag ctggcgacga
     1261 agggaaacga acgcgcgcgc gacagccggc cataggtcgg cttcaatcca aagattccgc
     1321 agaacgacga cggcacccgg atcgagccgt tggtatcgga ccccagcgcg atcggcacca
     1381 gaccgccgcc gaccgcggcg ccggagccgc ccgacgaacc gccggtcatc cgtgtcacat
     1441 cgtgcggatt gcgcgagggg ccgtcatgcg cattctcgcc ggtgaagtcg taggcgtatt
     1501 cgcccatgtt gagcgcgccg accagcacgg cgccggccgc ttccattcgc tcgatcaagg
     1561 tggcgtcgcg cttggaggcc gcaaggtcgc gattgatcct ggatccggcc cgggtcggca
     1621 ggtcctccac atcgaacagg ttcttcaccg cgaacggcac gccggcgagc ggcccggcat
     1681 tcttgccggc ggcaatcgcg gcgtccactg tgcgcgcccg ggcgcgggcg cggtcggcgg
     1741 tcacgtccgt gaacgcgttc aaaaccgggt tatgcttcgc gatccgcgcc agcgcggctt
     1801 cggtcacacc gagcgccgaa agcttcttgc ccgagatggc ctgcgcgatt tccgaggccg
     1861 actgtccgtc gaggtttgca ctcatcgcgg tatcaggccg taaagacact ggcgggctcg
     1921 gtctcgtccg gcagcgcgaa ttcatcgacc agccgagcga gcctcaggga gacctcgaga
     1981 ttggccttga ctgccggccg ccacgcgtct tccaccggca gcgccagcgc cttcgagacg
     2041 gcgtcgatat aatcgtccag gggatcggca gccatttcac tcccactcgt ttatccggtg
     2101 aacagagcta gtgagccggc aacggcggat gcggaatcgc cgtcagcagt tcctttgtat
     2161 aggcgtcctg cggatcgccc aacacgcgct ccgacagacc ctgttcaacg attcgtcccg
     2221 accgcatcac aatgacacga tcgcacagca accgcaccac attcaaatcg tgcgacacga
     2281 acaaatagct catgccc
//
LOCUS       DATMJB010000131         2892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_693293, whole genome shotgun sequence.
ACCESSION   DATMJB010000131 DATMJB010000000
VERSION     DATMJB010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693293"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VNU48_03000"
     CDS             <1..231
                     /locus_tag="VNU48_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10274.1"
                     /translation="GDVITTGRAESWASDYVARVALHRDQIRAETNKLDWLFSTHTTS
                     RSAAELLLFLHGGMMVSKGSARAASVKVGRGA"
     gene            228..>2892
                     /locus_tag="VNU48_03005"
     CDS             228..>2892
                     /locus_tag="VNU48_03005"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HWI10275.1"
                     /translation="MIAGLPLTFAEPFLLLGLLSLPVLWWLLRVMPPRPRRIEFPPTR
                     LLFDIAPKEETPSRTPWWLTALRLLAAALVILAAAGPIWNPKTGVGGSSAPLAILIDD
                     GWSAASSWDTRIKAADELIANADNDRRGVALVPLSEPTRDITIMPAGTARVALRQLAP
                     KPYSIERIETLGIFERFLKTTGDCEIAWLTDGVDTGRGPEFLDNLAKTIGDRALTVYE
                     GAPSPLALAAAENAAARMTVKVMRGNGGVAAGVVRALDSKGSPIGESRYTFGPNDRET
                     EAAFNLPVELRNDIARLEISGERSAGAVQLLDKRWRRRAIGVVTGATNDTAQPLLAST
                     FYLTRALAPFADVRLGDRGSPQQAITQFLDQKLPMIVLADVGTLSPEIRERINAWIEQ
                     GGVLVRFAGPRLAQADDDLVPVKLRRGGRSLGGSLTWEKPQHLAAFAADGPFAGVAVP
                     KDVTVNRQVLAEPNAALSARSWASLEDGTPLVTGERRGKGMVSLFHVSADMRWSDLPM
                     SGAFVEMLRRVVDMSGYTSTPGAGPAGETNAETVAPLRTLDGFGAFGPPPSTAKPLPA
                     DFRDRATAEHPPGFYGPADGPLAVNALAAADRIAPLDTSGLRARHATYTNAEPRDLRG
                     MLLSSALALFLIDAIIVAMLGAGIAALLRRRAATAVLAFAFALTAVLATPSPLRADNN
                     DDFAIKSVAQTRLAYVVTGNADVDSIVKAGMAGLTLFLAQRTALEAGDPVGIDPARDE
                     LAFFPLVYWPVVPGASKPPQDAINRIDAYMKQGGTVVFDTRDAIEAPPGDNGASQTPG
                     MLALRNILSSLDVPELEPVPREHVLTKTFYLLRDFPGRFNSGQTWVETLPREDDDEAA
                     SRPARGGDGVSPIIITSNDLAGAWAMRADGQP"
BASE COUNT          453 a         1004 c          962 g          473 t
ORIGIN      
        1 ggcgacgtca tcaccaccgg gcgcgccgag agctgggcca gcgactacgt cgcgcgcgtc
       61 gccctgcatc gcgaccagat tcgcgccgag accaacaagc tcgactggct gttctcgacc
      121 cacaccacca gccgctcggc cgccgaactg ctgctgttcc tgcacggcgg catgatggtc
      181 agcaagggaa gcgcgcgtgc cgcgagcgtc aaggtggggc gcggcgcatg atcgcaggcc
      241 tcccgcttac gttcgccgaa ccgtttctgt tgctgggact gctgagtctg ccggtattgt
      301 ggtggctgtt gcgggtgatg ccgccgcggc cgcgtcggat cgagtttccc ccgacccggc
      361 tgctgttcga catcgcgcca aaggaagaaa cgccgtcgcg cacgccatgg tggctgaccg
      421 ccctgcgcct gctggcggcc gcgctggtaa ttttggccgc cgccggcccg atctggaatc
      481 caaagaccgg cgttggtggc agcagcgcgc cgctggcgat cctgattgat gatggctgga
      541 gcgcggcctc gagctgggac acccggatca aggccgccga tgaattgata gccaatgccg
      601 acaacgaccg ccgcggcgtc gcgctggtgc cattgtccga gccgacgcgc gatatcacga
      661 tcatgccggc aggcaccgcg cgggtggcgt tgcgccagct cgctcccaaa ccctattcga
      721 tcgagcggat tgaaaccctt ggcatcttcg agcgcttttt gaagaccacc ggcgattgcg
      781 aaatcgcctg gctgaccgat ggcgtcgata ccgggcgcgg accggaattt ctcgacaatc
      841 tcgccaagac tatcggcgat cgcgccttga cggtctacga gggcgcgccc tctccgctgg
      901 cgctggccgc cgccgaaaat gccgcggcca ggatgacggt gaaagtgatg cgcggcaatg
      961 gcggcgtcgc cgccggcgtc gtgcgcgcgc tcgactccaa gggatcgccg attggcgagt
     1021 cgcgctatac ttttgggccg aacgatcgcg agaccgaagc cgcgttcaac cttccggtcg
     1081 aattgcgcaa cgatatcgcg cggcttgaaa tatccggcga gcgatccgcg ggcgcggtgc
     1141 aactgctcga caagcgctgg cgtcgccgcg cgatcggcgt cgtcaccggt gcgaccaacg
     1201 acaccgcgca gccgctgctg gcctcgacct tctatctcac ccgcgcgctg gcgccgttcg
     1261 ccgatgtcag gttaggggat cgcggctcgc cgcaacaggc gatcacgcaa ttcctcgacc
     1321 agaagctgcc gatgatcgtg ctggcggatg tcggcacgct gtcgcccgaa attcgcgaac
     1381 gcatcaacgc atggatcgaa cagggtggcg tgctggtacg ctttgccgga ccccggctgg
     1441 cgcaggccga tgacgatctg gtgccggtca aattgcgccg cggcggacgc agccttggcg
     1501 gcagcctcac ctgggagaag ccgcagcatc tcgcggcctt cgcggccgac ggaccgttcg
     1561 ccggcgtcgc ggtgccaaag gatgtcaccg tgaaccggca ggtactggcc gaacccaacg
     1621 cggcgctgtc ggccaggagt tgggcctcgc tcgaggatgg cacgccgctg gtgaccggcg
     1681 aacgtcgcgg caagggcatg gtcagcctgt tccatgtcag cgctgacatg cgctggtccg
     1741 atttgccgat gtccggagct ttcgtcgaga tgctccggcg cgtcgtcgat atgtccggtt
     1801 acacttccac gcccggcgcg ggtccggcgg gtgaaaccaa tgccgagacg gtggcgccgc
     1861 tgcgcacgct cgacggcttc ggcgcgttcg gcccgccgcc ctcaaccgca aaaccgctgc
     1921 ccgccgactt ccgcgaccgc gcgacggcag agcatccgcc cggcttctat ggcccggccg
     1981 acggcccgct cgccgtaaac gcgctggcgg cggcggaccg catcgcgccg ctcgacacct
     2041 ccggcctgcg cgcgcggcac gcgacctata ccaacgccga gccgcgcgat ctccggggta
     2101 tgctgctgtc gtcggcattg gcgctgttcc tgatcgacgc catcatcgtc gccatgctcg
     2161 gcgccggcat cgcggcattg ctgcggcggc gcgcggccac ggccgtgttg gcgttcgcat
     2221 tcgcgctgac ggcggtgctg gcgacgccgt cgccgctgcg cgccgacaac aacgacgatt
     2281 tcgccatcaa gtcggtggcg caaacccggc tggcttacgt cgtcaccggc aatgcggatg
     2341 tggactccat cgtcaaggcg ggcatggccg gcctgacatt gttcctggcg cagcgcaccg
     2401 cgctggaagc cggcgatccc gtcggcatcg atccggcgcg cgacgaactc gccttcttcc
     2461 cgctggtcta ctggccggtg gtacccggcg cgtcgaagcc gccccaggac gccatcaacc
     2521 gcatcgacgc ctacatgaag cagggcggca cggtcgtgtt cgacacccgc gacgccatcg
     2581 aggcgccgcc cggcgacaat ggcgcctcgc agacgccggg catgctggcg ctgcggaata
     2641 tcctttcctc gctcgatgtt cccgagcttg agccggtgcc gcgcgaacac gtgctgacga
     2701 aaacctttta tctgctgcgc gacttcccgg gccgcttcaa ttccggccag acctgggtcg
     2761 agaccctgcc gcgcgaggac gacgacgaag cagcatcccg gccggcgcgc ggcggcgacg
     2821 gcgtctcgcc gatcatcatc acctcgaacg atctcgccgg cgcatgggca atgcgggcgg
     2881 atggccagcc ga
//
LOCUS       DATMJB010000132         2177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_752790, whole genome shotgun sequence.
ACCESSION   DATMJB010000132 DATMJB010000000
VERSION     DATMJB010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752790"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VNU48_03010"
     CDS             complement(<1..360)
                     /locus_tag="VNU48_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HWI10276.1"
                     /translation="MSTTTTTLAAQPAVHRAVAPRTALAATAEPAWVRRTLIGVALAF
                     LAFFLFVPLAAVFFEAFKKGWGVYLAAITEPDAVSAIWLTLIAAAISVPLNLVFGIAA
                     AWCIAKFDFRGKNVLLTL"
     gene            complement(368..1210)
                     /gene="cysT"
                     /locus_tag="VNU48_03015"
     CDS             complement(368..1210)
                     /gene="cysT"
                     /locus_tag="VNU48_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HWI10277.1"
                     /translation="MFLSRTLLRRHSVLPGFDLALGFTLLYLGLIVLVPLSAAFLKTF
                     TMTWPAFWDAVTSPRVLASYRLTFGASLAAALLNSVFGLVVAWVLVRYEFPFKRLIDA
                     LVDLPFALPTAVAGIALTSLYAANGWIGQYLALVGIKVSYTPLGVFVALTFIGLPFVV
                     RTVQPVLEDLQKELEEAAATLGATRAQTFTRVILPVLTPALLTGFALAFARALGEYGS
                     VIFIAGNMPMISEITPLLIITKLEQYDYQGATAIAVVMLVAAFALLLAINLLQAWTRK
                     RQGR"
     gene            1380..1657
                     /locus_tag="VNU48_03020"
                     /pseudo
     CDS             1380..1657
                     /locus_tag="VNU48_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184450.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1779..>2177
                     /locus_tag="VNU48_03025"
     CDS             1779..>2177
                     /locus_tag="VNU48_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018432960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10278.1"
                     /translation="MRSVFVLAFAAAISTSVLAQSDKDHAAHHPGGASAPAAKSTPKA
                     APAAPAQMDMQMKSMQEMHEKMMAAKTPEERQALMTEHMKSMQGGMAMMGQMKAPDPK
                     GGGKSMSPEMMGRRMDMMEMMMQMMMDREAA"
BASE COUNT          421 a          691 c          739 g          326 t
ORIGIN      
        1 cagcgtcagc agcacgttct tgccgcggaa gtcgaacttg gcgatgcacc atgccgcggc
       61 gatgccgaac accaggttga gcggcaccga gatcgccgcg gcgatcagcg tcagccagat
      121 cgccgacacc gcgtcgggct cggtgatcgc cgcgaggtaa acgccccagc ctttcttgaa
      181 ggcctcgaag aacaccgcgg cgagcggcac gaacagaaag aacgcgagga acgcgagcgc
      241 cacgccgatc agcgtgcggc gtacccaagc cggttcggcg gtggcggcca gcgccgttcg
      301 cggcgcgaca gcccggtgca cggcgggctg cgccgccagg gtggtggtgg tcgtcgacat
      361 gccggtttca gcgcccctgg cgcttgcgcg tccaggcctg cagcaggttg atcgcgagca
      421 gcagcgcgaa cgcggcgacc agcatcacca ccgcaatggc ggtcgcgccc tggtagtcgt
      481 attgctccag cttggtgatg atcagcagcg gcgtgatctc cgagatcatc ggcatgttgc
      541 ccgcgatgaa gatcaccgag ccgtactcgc ccagtgcacg tgcgaacgcg agcgcgaagc
      601 cggtcagcag ggctggcgtc agcaccggga ggatcacgcg ggtgaaggtc tgagcgcgcg
      661 tcgcgcccag cgtggccgcg gcttcttcaa gctccttctg caggtcttcg aggaccggtt
      721 gcaccgtgcg cacgacgaac ggcaggccga tgaaggtcag cgccacgaac acacccagcg
      781 gcgtgtacga gaccttgatg ccgaccagcg caaggtactg gccgatccag ccgttggcag
      841 cgtacagcga ggtcagcgcg atgcccgcca ccgcggtggg cagcgcgaac ggcaggtcca
      901 ccagcgcatc gatgaggcgc ttgaacggga actcgtagcg caccagcacc caggccacga
      961 ccaggccgaa caccgagttc agcagcgccg ccgcgagcga ggcgccgaag gtcagccggt
     1021 acgacgcgag gactcgcggc gaggtgaccg cgtcccagaa cgccggccag gtcatcgtga
     1081 acgtcttcag gaacgccgcc gacagcggga ccaggacgat cagtccgagg tacagcagcg
     1141 tgaagcccag cgcgaggtcg aaaccgggca gcacgctgtg gcgtctgagc agggtgcggg
     1201 acaggaacac ggtgttcttc agcgctttcg acgtgaacgg caacgagatc ggtgcgcgag
     1261 catcgcgggc atcgcgcgac gcaagggcga aacgccggat gctacggacg cggtcgttta
     1321 caggaaacga ctgaaattca acttgcttat gcgcaaatca attaagcgcg ctctttctcg
     1381 agtgcacacg ccgcggcgtg ccgccgccga ccgtcagcct gttcggttcg cagttcctta
     1441 cctggcgcgg cattccgctg atccctcccg tcccttctga caaggtgccg gtggccgacg
     1501 gcaagaccaa ggtcatctgc tgcgcgccag cgacaagcgt cagggtgtgg tcggcctgta
     1561 ccagccgggg ctggcgggcg cgcagagccc ggggctgtcg gtgcgcttca cgggcatcaa
     1621 ccgcaatgcg atcgcgtcgt acctgatctc gttgtactga ccttgtacgg gtctggtcgg
     1681 gtgccgactt ccaggcgtgg cgcggcttgc tgccttgacg cacatcaagg cgcaagtcga
     1741 aatgcgtgcg aagcttatcc ggaactctgg agtctcctat gcgttccgtc ttcgttctcg
     1801 ccttcgccgc ggccatttcc accagcgtgc tggcgcaatc cgacaaggac catgctgcgc
     1861 accaccctgg aggtgcctcg gcgccggctg ccaagtcaac gcccaaggct gcccccgcgg
     1921 ctcctgcgca gatggacatg cagatgaagt cgatgcagga gatgcacgaa aagatgatgg
     1981 ccgcgaagac gcccgaggag cgtcaggcgc tgatgaccga gcacatgaag tccatgcagg
     2041 gcggcatggc gatgatgggt cagatgaagg cacccgaccc caaaggcggc ggcaagtcca
     2101 tgagccccga gatgatgggc aggcgcatgg acatgatgga aatgatgatg cagatgatga
     2161 tggaccgcga ggcggcc
//
LOCUS       DATMJB010000133         3088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_396699, whole genome shotgun sequence.
ACCESSION   DATMJB010000133 DATMJB010000000
VERSION     DATMJB010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396699"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="VNU48_03030"
     CDS             <1..1401
                     /locus_tag="VNU48_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWI10279.1"
                     /translation="GFDIIFFWVARMIMMTLHFTGKVPFRDVYIHGLVYDAHGHKMSK
                     SEGNVIDPVDLIEGVDLDTLIRKSTVGLRRPETAPKVAARIAKEFPQGMPAYGADALR
                     FTMASYASLGRQINFDTKRCEGYRNFCNKLWNATKFVLMNCQGQDCGLKEHTKAECAP
                     GAPFHGYMRFSPADKWISSELQRVEAAVEQGFADYRLDNVANAIYAFVWDEYCDWYIE
                     IAKTQLAVAKDSGDEGAQRATRRTLIRVLETVLRLMHPIMPFITAELWESVAPVAGRG
                     RVAAESRGESADGAGIVTAPYPKAQLDRVDADADAWVAKLKSVVGTCRALRSEMSLAP
                     SARVPLYVIGDDAFVGQAEPLLKALAKLSEVKRFASDPAFADATRSAAVAVAGDARLA
                     LHVEVDIGAETERLGKEIARLQGEIAKARAKLGNEGFVARAPAAVVAQEQQRVADFTA
                     NVARLQDQLGRLASSP"
     gene            complement(1343..2320)
                     /locus_tag="VNU48_03035"
     CDS             complement(1343..2320)
                     /locus_tag="VNU48_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI10280.1"
                     /translation="MDLLIIEPLEAEVMQWLESRYAIRYAPELAGDPRAFRQALYNVR
                     ALVVPPSVTIDAQALHYAPVLRAVGRVSAGAENIDLDACARARVEVVRGLTATAQAEA
                     EFMIGALLSLLRRVPVVGSDGMLVGRELGAATVGLIGMAPAARTMTQMLAGFGSTMVG
                     YDPALHASDSVFERWKVKPLGLRELLEQSDAVCVQLSYFSRYHGLLGERFLPFCKPNQ
                     VIVSIAHSALFDEASLAEALTGGRLAAAWLDSLEPGALDEGRPLHGIDNLQITPRVAS
                     TTRESRLRSAWAVARRIDEMLSAPGGSARGFKATMPGDPADLAAEPHSP"
     gene            2423..>3088
                     /locus_tag="VNU48_03040"
     CDS             2423..>3088
                     /locus_tag="VNU48_03040"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HWI10281.1"
                     /translation="MLVTKAIFPVAGLGTRFLPATKAQPKEMLPVVDKPLIQYAVEEA
                     YAAGVREMIFVTGRHKRPIEDHFDMTFELEVALEQAGKQELLEVVRGVKPDDMECIYV
                     RQAQALGLGHAVLCGQRLVGNNPFAVLLADDLMVGTKPILKQMVEQYEEWRVSILAVQ
                     EVPAEHTRRYGIVDGTPVNDHLMDVSRIVEKPAPEDAPSRLGVAGRYILTPGVFHEIA
                     NQQR"
BASE COUNT          528 a         1014 c         1078 g          468 t
ORIGIN      
        1 ggcttcgaca tcatcttctt ctgggtcgcc cggatgatca tgatgacctt gcacttcacc
       61 ggcaaggtgc cgttccgcga tgtctacatc catggcctgg tgtacgacgc gcacggccac
      121 aagatgagca agagcgaagg caacgtgatc gacccggtcg acctgatcga gggcgtcgat
      181 ctcgacacgc tgatccggaa gtcgaccgtc gggctgcgcc ggcccgagac ggcgccgaag
      241 gtcgcggcgc gcatcgcgaa ggagttcccg cagggcatgc cggcctacgg cgccgatgcg
      301 ctgcgcttca cgatggcgtc gtatgcgagc ctgggccgcc agatcaactt cgacaccaag
      361 cgctgcgagg gctaccgcaa cttctgcaac aagctctgga acgcaaccaa gttcgtgctg
      421 atgaattgcc aggggcagga ttgcgggctg aaggagcaca ccaaggccga gtgcgcgccg
      481 ggtgcgccgt tccacggcta catgcgcttc agcccggccg acaagtggat cagcagcgag
      541 ctgcagcgcg tcgaggcggc ggtcgagcag ggctttgccg actaccggct cgacaacgtc
      601 gccaacgcga tctacgcctt cgtctgggac gagtactgcg actggtacat cgagatcgcg
      661 aagacgcagc tcgccgtcgc gaaggacagc ggcgacgaag gcgcgcagcg cgcgacgcgc
      721 cgcacgctga tccgcgtgct cgagacggtg ctgcggctga tgcacccgat catgccgttc
      781 atcaccgccg agctctggga atcggtggcg ccggtggcgg gccgcgggcg ggtcgctgcc
      841 gaatcacgcg gcgaaagcgc cgacggcgcg ggcatcgtca cggcgcccta cccgaaggcg
      901 caactcgatc gcgtcgatgc cgacgccgat gcctgggtcg ccaagctgaa gagcgtggtc
      961 ggcacctgcc gcgcgctgcg cagcgagatg agcctggcac ccagcgcgcg cgtgccgctc
     1021 tacgtgatcg gcgacgacgc gttcgtcggg caggccgagc cgctgctgaa ggcgctcgcg
     1081 aagctgtcgg aggtgaagcg ctttgccagc gaccccgcgt tcgccgacgc gacgcgcagc
     1141 gccgcggtcg cggtggccgg cgatgcgcgc ctggcgctgc atgtcgaggt cgacatcggt
     1201 gccgagaccg agcgcctggg caaggagatc gcgcggctgc agggcgagat cgcgaaggcg
     1261 cgggccaagc tcggcaacga gggcttcgtc gcgcgcgccc ccgcggcggt ggtggctcag
     1321 gagcagcagc gcgtcgccga tttcacggcg aatgtggctc ggctgcaaga tcagctgggt
     1381 cgcctggcat cgtcgccttg aagccgcgcg cgctgccgcc cggcgcgctc agcatctcgt
     1441 cgatgcgcct ggccacggcc caagcgctgc gcaggcgcga ctcgcgcgtc gtgctcgcca
     1501 cgcgcggcgt gatttgcagg ttgtcgatgc cgtgcagcgg ccggccctca tcgagtgcgc
     1561 cgggctccag gctgtcgagc caggccgccg cgaggcgccc gccggtcagc gcctcggcca
     1621 gcgaggcctc gtcgaacagc gccgagtgcg cgatgctgac gatcacctgg ttcggcttgc
     1681 agaacggcag gaagcgttcg ccgagcaggc cgtgatagcg gctgaaatag ctgagctgca
     1741 cacacaccgc gtcggactgt tcgagcagtt cgcgcaggcc cagcggcttg accttccagc
     1801 gttcgaagac gctgtcgctg gcatgcagcg ccgggtcgta gccgaccatc gtcgagccga
     1861 agccggcgag catctgcgtc atcgtgcggg cggccggcgc catgccgatc aggccgaccg
     1921 tggcggcacc gagctcgcgc cccaccagca tgccgtccga gccgaccacc ggcacgcgcc
     1981 gcagcagcga cagcaacgcg ccgatcatga actcggcctc ggcctgcgcg gtcgcggtca
     2041 ggccgcgcac gacctcgacc cgggctcgcg cgcacgcatc gaggtcgatg ttctcggcgc
     2101 cggcgctgac gcggccgacc gcgcgcagca ccggcgcgta atgcagcgcc tgcgcgtcga
     2161 tcgtcaccga cggcggcacg accagcgccc gcacgttgta cagcgcctgg cggaacgcac
     2221 gcgggtcgcc ggccagctcg ggcgcatagc ggatcgcgta gcgcgattcg agccactgca
     2281 tgacctcggc ttccagcggt tcgatgatca gcagatccat gcgcttgagg gcatttctga
     2341 ggctttgtcc gacgaaccct caacggcacg catgccagaa tgaaggcaca ccgtcagctc
     2401 gggcgaaggg gattgtaagt aaatgttagt caccaaagcg atttttccag tggccggtct
     2461 aggcactcgt ttcttgccgg ccaccaaggc gcagcccaag gagatgctgc cggtggtgga
     2521 caagcccttg atccaatatg cggtcgagga ggcttatgcc gccggcgtgc gggagatgat
     2581 cttcgtcacg gggcgccaca agcgccccat cgaagatcac tttgacatga ctttcgagtt
     2641 ggaggtagcc ctcgaacagg cgggtaagca agagttactg gaggtcgtgc gcggcgtcaa
     2701 gcccgacgac atggagtgca tttatgtgcg ccaagcccag gcgctgggcc ttggtcacgc
     2761 ggtgctgtgt gggcagcggc tggtgggcaa caacccgttc gcggtgttgc tggccgacga
     2821 cttgatggtg ggtacgaaac cgatcctgaa gcagatggtc gagcagtacg aggagtggcg
     2881 ggtgtcgatt ctggcggtgc aggaagtgcc ggccgagcac acccgccgct acggcatcgt
     2941 cgacggcacg ccggtcaacg accacctgat ggacgtgagc cgcattgtcg agaagccggc
     3001 ccccgaagac gcgccgtcac gcttgggcgt ggccggccgc tacatcctga cgccgggcgt
     3061 atttcacgaa atcgcgaacc agcagcgt
//
LOCUS       DATMJB010000134         4515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_811701, whole genome shotgun sequence.
ACCESSION   DATMJB010000134 DATMJB010000000
VERSION     DATMJB010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811701"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /locus_tag="VNU48_03045"
     CDS             complement(<1..665)
                     /locus_tag="VNU48_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HWI10282.1"
                     /translation="MITGANAITRRALVTAALVLAAAAAQAGRSCENRAPTVDAIQRS
                     MLLAEHTARALDQSGAQVVVLARAGQNLSEYHLRYSHLGFAYRDAEQHWRVVHKLNQC
                     GSANGSLYRQGLGEFFLDDLYEYEAAIVVPRPDVQARLLPALQDNARVAQLNTPAYSI
                     LAYPWAQTYQQSNQWAIETLAMTQDPAAQTRVRAQAWLQLQGYQPTTLHIGPLKRLGG
                     RLTA"
     gene            complement(662..1084)
                     /locus_tag="VNU48_03050"
     CDS             complement(662..1084)
                     /locus_tag="VNU48_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10283.1"
                     /translation="MSTHSFSIRRCAVTALLAASMAAAQAQSGLSEASALSALPIAVS
                     VAAPVVVLSAGATLTVVAVEASAVGSVWVLERASDGARASVRLGSEAAGALSVAAGTV
                     VVVTAVSTGWVLSTASRAIAFVPNEIGAALLYNERLTR"
     gene            complement(1116..1538)
                     /locus_tag="VNU48_03055"
     CDS             complement(1116..1538)
                     /locus_tag="VNU48_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HWI10284.1"
                     /translation="MHPTPIRQDTRAWKLQVWVSFGLAALLCGTGLAYLPGTDLDRAF
                     MVMGYMFCLSTAFALAKYVRDNEAATTDTPLWGLVVWGGFGLAMALTAWGLWRMDVNP
                     TYKAFLGVSWLFLISTVFTLAKTLRDAHEALLAAREIG"
     gene            1675..2421
                     /locus_tag="VNU48_03060"
     CDS             1675..2421
                     /locus_tag="VNU48_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI10285.1"
                     /translation="MSTTHDLVSALKAELKAAGITYAELATQLGMAESSIKRIFAKAD
                     MPLSRIDEVLRVLKMDFAELARQVVDAQPLRRELTLEQETAVVADRKLMLLAICCVSQ
                     WTFEQMVATYAITDAECVRYLLQLDKLGFIELRPLNRYRLKVAKGFRWRPHGPVMAYF
                     REHVADDYYSGGFDGEGETLMLVHGHISPAQAVTFVERLQRVGQDFAQQHLADQKLSA
                     EHKRPYTLVVGMRSWLFAPFRDLKRMPAAR"
     gene            complement(2396..3586)
                     /locus_tag="VNU48_03065"
     CDS             complement(2396..3586)
                     /locus_tag="VNU48_03065"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWI10286.1"
                     /translation="MDAQSAVSRGPLAGLKVVELGQLIAGPFAAKTLADFGAEVIKVE
                     PPGGGDPLRQWRLLHDGTSVWWQVQSRNKQSVALDLKDEAARAIVRGLIGEADVLIEN
                     FKPGVMEAWGLDYARLSAANPGLIMLRISGYGQTGPYRDRPGFGVVAEAMGGLRHLTA
                     EPGRVPVRVGVSIGDTLASLHGVIGILMALQHRHASVSTDAPKGRGQVVDVALYEAVF
                     NCMESLLPEYSAFGAVRAAAGSALPGIAPSNAYPCADGWVLVAGNGDSIFKRLMSAIG
                     RDDLGADAALAGNAGRVARVAEIDSAIGAWTAPRSVAEVLDALNAAAVPAGRIYTAKD
                     IAEDPHYRARGMLQRITTADGLALDVPGIVPKLSLTPGAITRRAPRLGEDNDAVLSAR
                     PASF"
     gene            complement(3673..>4515)
                     /gene="rlmJ"
                     /locus_tag="VNU48_03070"
     CDS             complement(3673..>4515)
                     /gene="rlmJ"
                     /locus_tag="VNU48_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HWI10287.1"
                     /translation="MLAYRHAFHAGNHADVLKHVVLTRVLRYLNGKDKGYRFIDTHAG
                     AGGYSLEGRFAQKKGEFEQGIARLWDRDDLPPAVADYVQLVRRFNPDGRLEQYPGSPA
                     FAQMLLRAQDQLRMFELHPTDHRILQSYIGALKGAEVYDTDGFDGLKGQVPPSSRRGV
                     VLMDPSYEGHKDYSRVIASLREALARFAEGVYLVWYPQVSKLEAAQLPKRLEALAPKG
                     WLHARLTVQQPDQQGFGLAGSGMFVINPPFTLHDELLKVLPYLTDVLGQYDGANYLLE
                     QRPA"
BASE COUNT          687 a         1579 c         1579 g          670 t
ORIGIN      
        1 gcggtcagcc ggccgcccag gcgcttcagc ggcccgatgt gcagcgtcgt cggctggtag
       61 ccctgcagct gcagccaggc ctgggcccgc acccgcgtct gcgcggccgg gtcctgcgtc
      121 atcgccagcg tctcgatcgc ccactggttc gactgctggt aggtctgcgc ccacgggtac
      181 gcgaggatgc tgtaggccgg cgtgttcagc tgcgcgacgc gcgcgttgtc ctgcagcgcc
      241 ggcagcagcc gcgcctgcac atcggggcgc ggcacgacga tcgcggcctc gtactcgtac
      301 aggtcgtcga ggaagaactc gcccagcccc tggcggtaca gcgagccgtt ggcgctgccg
      361 cactggttca gcttgtggac gacgcgccag tgctgttcgg cgtcgcggta cgcgaagccg
      421 aggtgcgagt agcgcaggtg gtactcgctc aggttctggc cggcacgcgc cagcacgacg
      481 acctgcgcgc cgctctggtc gagcgcacgc gcggtgtgct cggccagcag catcgagcgc
      541 tggatcgcgt cgacggtcgg cgcgcggttc tcgcagctgc ggccggcctg cgccgcggcc
      601 gcggcgagca ccagcgcggc ggtgacgagc gcgcggcgtg tgatcgcgtt cgcgccggtg
      661 atcatcgcgt caaccgctcg ttgtacagca gcgccgcgcc gatctcgttc ggcacgaagg
      721 cgatcgcgcg gctcgcggtc gacagcaccc agccggtgct gaccgcggtg acaacgacca
      781 ccgtgccggc cgccaccgac aacgcgccgg ccgcttccga gccgagccgc acgctggcgc
      841 gggcgccgtc ggaagcgcgc tccagcaccc agaccgaacc gaccgccgag gcctcgaccg
      901 cgaccacggt cagcgtcgcg ccggccgaca gcacgaccac cggtgccgcg accgagaccg
      961 cgatcggcag cgccgacaag gcgctcgctt cggacaggcc cgactgtgcc tgcgcggccg
     1021 ccatcgaggc ggcgagcagc gcggtcacgg cgcaacgacg gatggaaaag gaatgcgtac
     1081 tcatgtgaag ctcctgagag ggaatgggat gcctgtcagc cgatctcgcg cgcggccagc
     1141 agcgcctcgt gcgcatcgcg cagcgtcttg gccagcgtga acacggtcga gatcaggaac
     1201 agccagctga cgccgaggaa ggccttgtag gtcgggttca cgtccatgcg ccacaggccc
     1261 caggccgtca acgccatcgc caggccgaac ccaccccaga ccaccaggcc ccacagcggc
     1321 gtgtcggtgg tcgcggcttc gttgtcgcgc acgtacttgg cgagcgcgaa cgcggtggag
     1381 aggcagaaca tgtagcccat caccatgaac gcgcggtcca ggtcggtgcc cggcaggtag
     1441 gccaggccgg tgccgcacag cagcgcggcg aggccgaacg agacccagac ctgcagcttc
     1501 caggcgcgtg tgtcctggcg gatcggggtg gggtgcatgg ggtgctcttt cgtgcagtgg
     1561 ttgaacgtgg actcatcttc ggtaacgcca ccgtgggtat caatcacccg aaggagggtt
     1621 gcactcgtct gcccctctgg tatcttctga cgctactttt cgaggaggtt cgccgtgagc
     1681 accacgcacg acctggtcag cgcgctgaag gccgagctca aggccgccgg catcacctac
     1741 gccgaactcg cgacgcagct cggcatggcc gagtccagca tcaaacgcat cttcgcgaag
     1801 gccgacatgc cgctgtcgcg catcgacgag gtgctgcgcg tgctgaagat ggacttcgcc
     1861 gagctcgcgc gccaggtcgt cgatgcgcaa ccgctgcgcc gcgagctgac gctcgagcag
     1921 gagacggccg tggtcgcaga tcgcaagctg atgctgctgg cgatctgctg cgtcagccag
     1981 tggaccttcg agcagatggt cgcgacctac gcgatcaccg acgccgaatg cgtgcgctac
     2041 ctgctgcagc tcgacaagct cggcttcatc gaactgcggc cgctgaaccg ctaccggctg
     2101 aaggtggcga agggtttccg ctggcggccg cacgggccgg tgatggccta cttccgcgag
     2161 cacgtcgccg acgactacta cagcggcggc ttcgacggcg agggcgagac gctgatgctg
     2221 gtgcacggcc acatcagccc ggcacaggcg gtcacctttg tcgagcggct gcagcgcgtc
     2281 gggcaggact tcgcgcagca acacctggcc gaccagaaac ttagcgccga gcacaagcgg
     2341 ccgtacacgc tggtcgtcgg gatgcgctcg tggctgttcg caccgttccg cgacctcaaa
     2401 aggatgccgg ccgcgcgctg agcaccgcgt cgttgtcttc gcccaggcgc ggcgcgcgcc
     2461 gcgtgatcgc gcccggcgtc aggctcagct tcggcacgat gccgggcacg tcgagcgcga
     2521 ggccgtcggc ggtggtgatg cgctggagca tgccgcgcgc gcggtagtgc gggtcttcgg
     2581 cgatgtcctt cgcggtgtag atgcggcccg ccggcaccgc ggccgcgttg agcgcgtcca
     2641 gcacctcggc cacgctgcgt ggcgcggtcc aggcgccgat cgccgaatcg atctcggcga
     2701 cgcgcgcgac gcggccggcg ttgcccgcga gcgccgcgtc ggcacccagg tcatcgcggc
     2761 cgatcgcgct catcaggcgc ttgaagatcg agtcgccgtt gcccgccacc agcacccagc
     2821 cgtcggcaca tgggtaggcg ttgctcggcg cgatgccagg cagcgccgaa cccgccgccg
     2881 cgcgcaccgc gccgaacgcg ctgtactcgg gcagcaggct ttccatgcag ttgaacaccg
     2941 cctcgtacaa ggcgacgtcg acgacctgcc cgcgcccctt cggcgcatct gtgctgacgc
     3001 tcgcatgccg gtgctgcagc gccatcagga tgccgatcac gccgtgcaac gacgccagcg
     3061 tgtcgccgat gctcacgccc acgcgaaccg gcacgcggcc cggctcggcg gtcaggtggc
     3121 gcagcccgcc catcgcctcg gcgacgacgc cgaagcccgg ccgatcgcgg tacggcccgg
     3181 tctggccgta gccgctgatg cgcaacatga tcaggcccgg gttggcggcg ctcaggcgcg
     3241 cgtagtccag gccccaggcc tccatcacgc cgggcttgaa gttctcgatc aggacgtcgg
     3301 cctcgccgat cagcccgcgc acgatcgcgc gtgcggcctc gtccttcagg tcgagcgcga
     3361 cgctctgctt gttgcgcgac tgcacctgcc accacacgct ggtgccgtcg tgcagcagcc
     3421 gccattggcg cagtgggtcg ccgccaccgg gcggctcgac cttgatgacc tcggcaccga
     3481 agtcggccaa cgtcttcgcg gcgaacggcc ccgcgatcag ctggccgagc tcgaccactt
     3541 tcaggccggc gagcggcccg cgggaaacgg cagattgagc gtccatgacc gcgatggtag
     3601 tgtcgcggca cggccgaggt cgacgcgcgc gccgtcgcct ggcgggccga aatcgacggc
     3661 cagtcgtcgc gctcaggccg ggcgctgttc gagcaggtag ttcgcgccgt cgtactggcc
     3721 gagcacgtct gtcaggtaag gcaagacctt cagcagctcg tcgtgcaagg tgaacggcgg
     3781 gttgatcacg aacatgccgc tgccggccag gccgaaaccc tgctggtcgg gctgctgcac
     3841 cgtcagccgc gcatgcagcc agcccttggg cgcgagcgcc tcgaggcgct tcggcagctg
     3901 cgccgcctcg agcttgctga cctgcgggta ccagaccagg tagacgcctt cggcgaagcg
     3961 cgcgagcgcc tcgcgcagcg acgcgatcac gcggctgtag tccttgtggc cctcgtagct
     4021 cgggtccatc agcacgacgc cgcgccgcga cgatggcggc acctggccct tcaagccgtc
     4081 gaagccgtcg gtgtcgtaga cctcggcgcc cttgagcgcg ccgatgtacg actgcaggat
     4141 gcggtggtcg gtggggtgca gctcgaacat gcgcagctgg tcctgcgcgc gcagcagcat
     4201 ctgcgcgaac gccggcgagc cggggtactg ttcgagccgg ccgtcggggt tgaagcggcg
     4261 cacgagctgc acgtagtcgg cgacggccgg aggcagatcg tcgcggtccc acaaccgcgc
     4321 gatgccctgt tcgaactcgc ccttcttctg cgcgaagcgc ccttcgagcg aatagccgcc
     4381 ggcgccggcg tgcgtgtcga tgaagcggta acccttgtcc ttgccgttga gataacgcag
     4441 cacgcgggtc agcacgacgt gcttgagcac atcggcgtga ttgccggcgt ggaatgcgtg
     4501 gcggtaggca agcat
//
LOCUS       DATMJB010000135         4688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_396511, whole genome shotgun sequence.
ACCESSION   DATMJB010000135 DATMJB010000000
VERSION     DATMJB010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396511"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="VNU48_03075"
     CDS             complement(<1..1534)
                     /locus_tag="VNU48_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWI10288.1"
                     /translation="MRLNSIKLSGFKSFAEPTNFQLPGQLVGVVGPNGCGKSNIMDAV
                     RWVLGESKASELRGESMQDVIFNGSGSRKPASRASVELVFSNEDGRAGGQWNRFTEIA
                     VKRVLTRDGTSSYYINNQPVRRRDVQDVFLGTGLGPRAYAIIGQGTISRIIESKPEEL
                     RLFLEEAAGVSKYKERRRETETRLKDTRENLTRVEDILRELNSNLDKLEKQAEVASQY
                     RALQASGELKLHQLWFLKHRDAASEEARVKKAVAEAVNALEARTAELRHGEAELETIR
                     QAHYAASDELHAHQGALAEAALEVSRLEERIRYVVEGRARVEQRLLELKAQSEQWALR
                     QAEGRAEIERIAAQIAAADEQGELLAVQAEDLSGQLPNVEDALRAAQNASNEQRGKVS
                     AVQQQIQVLAAESRSVDDQKRQFNARRDKLAAERNQLAAPDVERLGELKQQHALADQA
                     FGVADARLHELGEQVPALDAARRAVQDSVNAESAKQADLSARRDALRALQEKVQTEGK
                     LKP"
     gene            1660..2133
                     /gene="queF"
                     /locus_tag="VNU48_03080"
     CDS             1660..2133
                     /gene="queF"
                     /locus_tag="VNU48_03080"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HWI10289.1"
                     /translation="MAKSATTKAAATQRRKPARPPSAPSRELQVFPNPAPRRDYVIQF
                     QIPEFTCHCPLTGQPDFAHFTIDMVADKLCVELKSLKMYMWSYRDEGAFHEKVTNDIV
                     DDIVRATHPRFIRLTAKWYVRGGIYTNVVAEHRQKGWKAQPAVELPAHAQATGLL"
     gene            2155..3354
                     /gene="dapC"
                     /locus_tag="VNU48_03085"
     CDS             2155..3354
                     /gene="dapC"
                     /locus_tag="VNU48_03085"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HWI10290.1"
                     /translation="MNPLLARLHPYPFERLRALTRDITPNPAFAPIGLGIGEPRHATP
                     ELIKQAVVANLKGLASYPATAGEPALREAIAAWLARRYGVAVDPLTQVLPVGGSREAL
                     FALAQTVIDPTRPGATVVCPNPFYQIYEGAALLAGAQVAFVNSDPARNFAADWASVPD
                     AVWARTQLLYVCSPGNPTGAVMPLAEWQRLFELSERHGFVIASDECYSEIYFHDEPPL
                     GSLEAATKLGRGFDRLIAFTSLSKRSNVPGMRSGFVAGDAAILKAFLLYRTYHGSAMG
                     PVVQQASIAAWNDEAHVVENRALYRTKFAQVTPLLAAVLPVALPDAGFYLWADVSRVA
                     PGGDDVTFALGLLAKYNVAVLPGSLLARESNGVNPGAGRIRLALVAPAEDCLAAARRI
                     VSFVQSH"
     gene            3365..4195
                     /gene="dapD"
                     /locus_tag="VNU48_03090"
     CDS             3365..4195
                     /gene="dapD"
                     /locus_tag="VNU48_03090"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HWI10291.1"
                     /translation="MTRPTHELESIIDLAWESRASINATNSPQIYEAVEAVIGELNAG
                     RLRVAQREGVGQWRVNQWVKKAVLLSFRLNDNKVMRAGDLGFFDKVSPKFAHLSEDEM
                     RATGVRVVPPAVARRGSYVAKNVVLMPSYVNIGAYVDEGTMVDTWSTVGSCAQIGKNV
                     HLSGGVGIGGVLEPLQANPTIIEDNCFVGARSEVVEGVIVEENSVISMGVYISQSTKI
                     YNRMTGEISYGRIPSGSVVVSGSLPANDGSHSLYCAVIVKRVTAETRAKTSINELLRS
                     "
     gene            4290..>4688
                     /locus_tag="VNU48_03095"
     CDS             4290..>4688
                     /locus_tag="VNU48_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HWI10292.1"
                     /translation="MSTGNMERVLRLMSEKAASDVYLSASSPILIKISGQLLPLSDQP
                     LTHTQPRQLLAELLTPSQLEELEDTGELNVGVGIPGVGSFRLSAFKQRGSIAAVFRCI
                     PFVIPSLESLNVPPALNTLVLEKRGLILMVG"
BASE COUNT          692 a         1649 c         1585 g          762 t
ORIGIN      
        1 agggcttgag cttgccctcg gtctggacct tctcctgcag cgcgcgcagc gcgtcgcggc
       61 gcgcgctcag gtcggcctgt ttcgcgctct cggcgttgac gctgtcctgc acggcgcggc
      121 gcgcggcgtc gagcgccggc acctgctcgc cgagctcgtg cagccgcgcg tcggcgacac
      181 cgaaggcctg gtcggccagc gcatgctgct gcttcaactc gcccaggcgt tcgacatcgg
      241 gcgcggcaag ctggttgcgc tcggcggcga gtttgtcgcg gcgcgcgttg aactggcgct
      301 tctggtcgtc gacgctgcgg ctctcggccg ccagcacctg gatctgctgc tgcaccgcgc
      361 tgaccttgcc gcgctgctcg ttgctggcgt tctgcgccgc gcgcagcgcg tcctcgacat
      421 tcggcaactg gcccgacagg tcttcggcct gcacggcaag cagctcgccc tgttcgtcgg
      481 ccgcggcgat ctgtgcggcg atgcgctcga tctcggcccg gccttcggcc tggcgcagcg
      541 cccattgctc gctctgcgcc ttcagctcga gcaggcgctg ctcgacgcgc gcgcggccct
      601 cgaccacata gcggatgcgc tcctcgagcc ggctcacttc gagcgcggcc tcggcgagcg
      661 cgccctggtg cgcgtgcagt tcgtcgctcg cggcgtaatg cgcctggcgg atcgtctcga
      721 gctcggcctc gccgtgacgc agctcggcgg tgcgcgcctc gagcgcgttg acggcctcgg
      781 ccacggcctt cttgacgcgc gcctcctcgc tcgccgcgtc gcggtgcttc aggaaccaca
      841 gctggtgcag cttgagctcg ccgctggcct gcagcgcacg gtactggctc gcgacctcgg
      901 cctgcttctc gagcttgtcg aggttgctgt tcagctcacg caggatgtct tcgacgcgcg
      961 tgaggttctc gcgtgtgtcc ttcaggcgcg tctcggtctc gcggcggcgt tccttgtact
     1021 tggatacgcc ggcggcctct tcgaggaaca ggcgcagctc ctcgggcttg ctctcgatga
     1081 tgcgcgagat cgtgccctgc ccgatgatcg cgtaggcgcg cgggccgaga ccggtgccga
     1141 ggaacacgtc ctgcacgtcg cggcgccgca ccggctggtt gttgatgtag tagctgctgg
     1201 tgccgtcgcg cgtcagcacg cgcttgacgg cgatctcggt gaagcggttc cactgcccgc
     1261 cggcgcggcc gtcctcgttg ctgaagacga gctcgacgct ggcgcgcgac gccggcttgc
     1321 gcgagcccga gccgttgaag atcacgtcct gcatcgactc gccgcgcaac tcgctggcct
     1381 tgctctcgcc cagcacccag cggaccgcgt ccatgatgtt ggacttgccg cagccgttcg
     1441 gcccgacgac gccgaccagt tgccccggca gctggaagtt ggtgggttcg gcgaaggact
     1501 tgaaaccgga gagcttgatc gaattcaggc gcattggcgg tgacaggctg gcgcctaagt
     1561 gcttgatttg tttgaataaa acccgcaagg tgcgggcctc ggagcagcgt cgatgatagc
     1621 atccgggcct tcgccgaaag cacccggaag ccctgcacca tggccaagtc agccaccacc
     1681 aaagccgctg cgacgcagcg ccggaaacct gcccgcccgc cgagcgcacc gagccgggaa
     1741 ctgcaggtgt tcccgaaccc ggcgccgcgg cgcgactacg tgatccagtt ccagatcccc
     1801 gagttcacct gccactgccc gctgaccggc cagcccgact tcgcgcattt cacgatcgac
     1861 atggtcgccg acaagctctg cgtcgagctg aagagcctga agatgtacat gtggagctac
     1921 cgcgacgagg gcgcgttcca cgagaaggtc acgaacgaca tcgtcgacga catcgtgcgc
     1981 gccacccatc cgcgcttcat ccgcctcact gcgaagtggt acgtgcgcgg cggcatctac
     2041 accaacgtcg tcgccgaaca ccgccagaaa ggctggaagg cgcagccggc ggtcgagttg
     2101 cctgcgcatg cgcaggccac cgggctgctc tgaggctgcg tcgataatcg gtcgatgaat
     2161 ccgctgctgg cgcgcctgca cccctacccg ttcgaacgcc tgcgtgcgct gacccgcgac
     2221 atcacgccga acccggcctt cgcgccgatc gggctgggca tcggcgagcc gcgccacgcg
     2281 acgccggaac tgatcaagca ggccgtcgtg gcgaacctga aaggcctggc gagctacccg
     2341 gccaccgccg gtgagccggc actgcgcgag gcgatcgccg cgtggctcgc gcggcgctac
     2401 ggcgtcgccg tcgacccgct gacgcaggtg ctgccggtcg gcggctcgcg cgaggcgctg
     2461 ttcgcgctgg cgcagaccgt gatcgacccg acgcggcccg gcgcgaccgt ggtctgcccg
     2521 aatccgttct atcagatcta cgagggcgcg gcgctgctcg ccggggcgca ggtcgcgttc
     2581 gtcaacagcg atcccgcgcg caacttcgcg gccgactggg ccagcgtgcc cgacgcggtc
     2641 tgggcgcgca cgcagctgct ctacgtctgc tcgcccggca accccaccgg cgcggtgatg
     2701 ccgctggccg aatggcagcg cctgttcgag ctcagcgaac ggcacggctt cgtgatcgcg
     2761 tcggacgagt gctattccga gatctacttc cacgacgagc cgccgctcgg ctcgctcgag
     2821 gcggcgacca agctcggccg cggcttcgat cgcctgatcg cgttcacgag cctgtcgaag
     2881 cgctcgaacg tgcccggcat gcgctcgggt ttcgtcgccg gcgacgcggc gatcctgaag
     2941 gcttttctgc tctaccgcac ctaccacggc agcgcgatgg gcccggtcgt tcagcaggcg
     3001 agcatcgcgg cctggaacga cgaggcccac gtggtcgaga accgcgcgct gtaccgcacg
     3061 aagttcgcgc aggtcacgcc gctgctcgcc gcggtgttgc cggtggcgct gcccgacgcc
     3121 ggcttctacc tctgggccga cgtgtcgcgt gtcgcgccgg gcggcgacga cgtcaccttc
     3181 gcgctcggcc tgctcgcaaa atacaatgtc gcggtgctgc cgggcagcct gctggcgcgc
     3241 gaatcgaacg gcgtcaaccc gggtgccggc cgcatccgcc tcgcgctggt cgcgccggcc
     3301 gaggactgcc tggccgccgc gcggcgcatc gtctcctttg tccaatccca ctgagcccac
     3361 acccatgacc cgacccaccc acgagctgga aagcatcatc gacctcgcct gggagagccg
     3421 cgcgtcgatc aacgccacca acagcccgca gatctacgag gccgtcgagg ccgtgatcgg
     3481 cgagttgaac gccgggcgcc tgcgcgtcgc gcagcgcgaa ggcgtgggcc agtggcgcgt
     3541 gaaccagtgg gtcaagaagg cggtgctgct gagcttccgc ctgaacgaca acaaggtgat
     3601 gcgcgccggc gacctcggtt tcttcgacaa ggtcagcccc aagttcgcgc acctgagcga
     3661 ggacgagatg cgcgccaccg gcgtgcgcgt cgtgccgccg gccgtggcgc ggcgcggctc
     3721 gtacgtggcg aagaacgtcg tgctgatgcc cagctacgtg aacatcggcg cgtatgtgga
     3781 cgaaggcacg atggtggaca cctggtccac cgtcggctcg tgcgcgcaga tcggcaagaa
     3841 cgtgcacctg tcgggcggcg tcggcatcgg cggggtgctc gaaccgctgc aggcgaaccc
     3901 caccatcatc gaggacaact gcttcgtcgg cgcgcgcagc gaggtcgtcg aaggcgtgat
     3961 cgtcgaggag aactcggtga tctcgatggg cgtgtacatc agccagagca cgaagatcta
     4021 caaccgcatg accggcgaga tcagctacgg ccgcatcccg tcgggctcgg tggtggtgag
     4081 cggctcgctg ccggcgaacg acggcagcca cagcctgtac tgcgcggtga tcgtcaagcg
     4141 cgtcacggcc gagacgcgcg ccaagaccag catcaacgag ctgctgcgca gctgatcacg
     4201 gccagcggcg ttgcgggcgc gccgcaccgc gcgcaccggc cccgaagcgt ggaatgatcg
     4261 acccgtctgc cagccttgaa aggatcgcga tgagtaccgg aaacatggaa cgcgtgctgc
     4321 gactgatgtc ggagaaggcg gcgtcggacg tgtacctgtc ggcgagctcg ccgatcctga
     4381 tcaagatcag cggccagctg ctgccgctgt cggaccagcc gctgacccac acgcagccgc
     4441 gccagctgct ggccgagctg ctgaccccca gccagctcga ggagctggag gacaccggcg
     4501 agctcaacgt cggcgtcggc attcccggag tcggcagctt ccggctctcg gcgttcaagc
     4561 agcgcggctc gatcgccgcg gtgttccgct gcatcccgtt cgtgatcccg tcgctcgaga
     4621 gcctgaacgt gccgcccgcg ctgaacacgc tggtgctcga aaagcgcggc ctgatcctga
     4681 tggtcggc
//
LOCUS       DATMJB010000136         2417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_871147, whole genome shotgun sequence.
ACCESSION   DATMJB010000136 DATMJB010000000
VERSION     DATMJB010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871147"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /gene="rfbA"
                     /locus_tag="VNU48_03100"
     CDS             <1..732
                     /gene="rfbA"
                     /locus_tag="VNU48_03100"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HWI10293.1"
                     /translation="ILLISTPQDTPRFESLLGDGRRWGLDIRYCVQPSPDGLAQAFIL
                     GRDFVGSQPSALVLGDNIFYGHDLQRILHAAAAREQGASVFAYHVTDPQRYGVVAFDA
                     QQRALSIEEKPRAPQSNYAVTGLYFYDEQVCDIAAHIKPSARGELEITDLNAIYLTQG
                     QLNVEIMGRGYAWLDTGTHDSLLEASQFIATLEKRQGLKVACPEEIAFRAGWISAEQL
                     EALALPMLKNGYGQYLQRVLVDRVY"
     gene            1248..>2417
                     /locus_tag="VNU48_03105"
     CDS             1248..>2417
                     /locus_tag="VNU48_03105"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HWI10294.1"
                     /translation="MINKRGTKSSAIPTTLCALFAATLSACGGGDAGSSTGTDSLAMG
                     LSDTSSMAQLQAAGGNGRGKGKGADLTAAAPAPVSAPAPAPAPAPAPAPAPALAPAPT
                     SLMPYIDVSKIPAAGTSYATARVMAANVADSSQVPAPADIGAFREPCNFSHMSFDDPI
                     VHPGQPGVSHLHTFFGNASVDAYSTAQSLSTSGNSSCAGGTLNRTAYWVPTLIDTKDG
                     TPVVPAGSAIYYKTGYLGVAPATVKPMPAGLRMVAGNPNATAPQNGVGRFACVGATAD
                     NTWRGYISSCPAGSQLVAEVAFPQCWDGVNLDSPDHKSHMAYASNGCPSTHPVPLPLV
                     SFEIHYNVTDSTQTARWRLSSDNYDPSLPGGYSSHGDWFMGWDPSIMSTFVAKCLD"
BASE COUNT          415 a          893 c          723 g          386 t
ORIGIN      
        1 atcctgctga tcagcacgcc gcaggacacg ccgcgcttcg agtcactgct cggcgacggc
       61 cgccgctggg gcctggacat ccgctactgc gtacaaccca gcccggacgg cctggcgcag
      121 gcgttcatcc tgggtcgcga cttcgtcggt tcccaaccca gcgcgctggt gctgggcgac
      181 aacatcttct acggccacga cctgcagcgc atcctccatg cggcggcggc ccgcgagcaa
      241 ggcgcctcgg tgttcgcgta tcacgtgacc gacccgcagc gctacggcgt ggtcgcgttc
      301 gacgcacagc agcgcgccct cagcatcgag gagaagccgc gcgcgccgca gagcaactac
      361 gccgtcacgg gcctgtactt ctacgacgaa caggtctgcg acatcgccgc ccacatcaag
      421 ccctcggcgc gcggcgagct cgagatcacc gacctgaatg cgatctacct gacgcaaggc
      481 cagctcaacg tcgagatcat ggggcgcggt tatgcctggc tcgacaccgg cacgcacgac
      541 agcctgctcg aggcgagcca gttcatcgcg acgctcgaga aacgccaggg cctgaaggtt
      601 gcatgccccg aagagatcgc cttccgcgcg ggctggatca gcgccgagca gctcgaggcc
      661 ctcgcgctgc cgatgctgaa gaacggctat ggccagtacc tgcaacgggt gctggtcgac
      721 cgcgtctact gagcgtcggc gccgcatgct ggcgccgcgt agccggaggt aaaccgcgtt
      781 tcgggatgcc gcggccggcg ccaacccgcg tcggtcatgt aagggcgcgc tacggcgcgg
      841 cccaagcgcg gcaccgaagt cgacaggctt gggcactttg gctgcggaac cggccgggca
      901 tgcgatctgc actccgccgc gcgtctccca ccgcttcatg ccggatctgc cggccttccc
      961 gacagcatgt ccccgctccc tgttcccgca ctcacgcccg tccgatcctt acgtcacgtt
     1021 acctgcgaaa catttgcagc aaaagaagcc tctcaagcac cggcgcctcg cgccgatacc
     1081 gatagcaacc cagcactgaa cctgtcttgc aggccggtgc tgatcagtgc ctcgcgaccc
     1141 ccgcgctccc agcgccatac cgacttccgt cggtccactc gtatcgccgc accgcagcgc
     1201 cgtcgatcca catcgacgga tgaaccgaca cacgagtgag acacgacatg atcaacaagc
     1261 gcggcaccaa gtcctccgcc attccgacca cgctgtgcgc cctgttcgca gcaactcttt
     1321 ctgcctgcgg cggcggcgat gccggctcgt ccaccggcac cgattcgctg gcgatgggcc
     1381 tgagcgacac gtcctcgatg gcccagctcc aggcggccgg cggcaatggg cggggcaagg
     1441 gcaagggggc cgatctcacg gctgctgcgc cggctccggt ttccgcacct gcacctgcac
     1501 ctgcacctgc acctgcacct gcacctgcac ccgcactcgc gccggctccg acctcgctga
     1561 tgccctacat cgacgtctcg aagattcccg ctgccggcac gtcgtatgcg acggcccgcg
     1621 tgatggccgc gaacgtcgcc gactcgagcc aggtgccggc accggcagac atcggcgcat
     1681 tccgcgagcc ctgcaatttc tcgcacatga gttttgacga tccgatcgtg cacccaggtc
     1741 agcccggggt atcccacctc cacaccttct tcggcaacgc atcggtcgac gcctattcaa
     1801 cggcccagtc gctctccacc tcgggcaact ccagctgcgc cggtggcacg ctcaaccgca
     1861 ccgcctattg ggtgccgacg ctcatcgaca cgaaggacgg aacgccggtc gtgccggcgg
     1921 gttcggcgat ctactacaag accggatatc tcggcgtggc ccccgcaacc gtgaagccga
     1981 tgcccgccgg cttgcggatg gtcgcaggca atccgaacgc gactgcgccg cagaacggcg
     2041 tcggacgttt cgcctgcgtc ggcgcgacgg ccgacaacac atggcggggc tacatctctt
     2101 catgcccggc cggctcacag ctggtggcgg aggtcgcgtt tccgcaatgc tgggacggtg
     2161 tgaacctcga ctcgccggat cacaagtcac acatggccta cgcgagcaac ggttgcccca
     2221 gcacccatcc ggtgccgctg cccctggtca gcttcgagat tcactacaac gtgaccgact
     2281 ccacccagac cgcgcgctgg cgtctgtcat cggacaacta cgaccccagc ctacccggcg
     2341 gctactcgtc gcacggcgac tggttcatgg gctgggatcc ttcgatcatg agcacgttcg
     2401 tcgcgaagtg cctggac
//
LOCUS       DATMJB010000137         3482 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_514903, whole genome shotgun sequence.
ACCESSION   DATMJB010000137 DATMJB010000000
VERSION     DATMJB010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514903"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="VNU48_03110"
     CDS             complement(<1..1136)
                     /locus_tag="VNU48_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWI10295.1"
                     /translation="MPAYKFEALDAAGKSTTGLLDAENAKAARSQLRAQALVPMAVTL
                     VASAGTQAEGLRFTRRVFNTTSLTVWTRQLAGLVGAGLPLERALTALSEEAEDPRQRE
                     LVAHLKSEVNGGSPFARALGSAPREFDEVYRGVVAAGEQSGALGKVLERLADDLEERQ
                     ELRSKLIGATLYPAIVSLIAIVIVIFLVTYVVPQVASVFSSSKRALPALTVAMLGLSA
                     FLRNWGWAVLLLIAAGVGLLLAMLRNEVFRQSFDAGWLELPLIGKLARGYNAARFAGT
                     LAMLAGAGVPILKALQAAAETLSNRAMRADAMEALVQVREGAPLASALAGKKRFPGLL
                     AMFSRLGEQTGQLPQMLDRAARQLGTEVQRRALQMATILEPLLIV"
     gene            complement(1142..2563)
                     /gene="gspE"
                     /locus_tag="VNU48_03115"
     CDS             complement(1142..2563)
                     /gene="gspE"
                     /locus_tag="VNU48_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HWI10296.1"
                     /translation="MGARHPLPYAYAKAHTLLLEDDGARLVLWAPESVGLPALGEVLR
                     LYDVDALEREAASSLANRIAVAYAGSESSAATVIGEVESAVDLSRMMQELPAIEDLLE
                     ASNDAPIIRMLNALLTQAAKDGASDIHIEPYERSSSVRFRVDGTLREVVQPNKALHAA
                     LISRLKIMAELDIAERRLPQDGRISLRIGGRAVDVRVSTLPSSHGERAVLRLLDKGES
                     KFTLEGLGMSGAVLQRFDKLVQQPHGIVLVTGPTGSGKTTTLYASLGRIDTATTNVLT
                     VEDPVEYELAGIGQTQVNPKIDLTFAKALRAILRQDPDVIMIGEIRDYETAQIAIQAS
                     LTGHLVLATVHTNDAPSTVTRMIDMGVEPFLLSSSLIGVLAQRLVRKLCPACKKIDAR
                     GRYHPVGCVECGNTGYKGRTGVYELMAVDDQVRALIHGRAAESQIFVAAEAAGLRSMR
                     EDGERLVAEGVTSPEEVMRVTRE"
     gene            complement(2577..>3482)
                     /locus_tag="VNU48_03120"
     CDS             complement(2577..>3482)
                     /locus_tag="VNU48_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HWI10297.1"
                     /translation="GSAALATGSSGVISALNSGLPNAGFNIGLAHKIGNFYTLGALAQ
                     ALSSTTGTNILSTPNLVTLDNEEARIVVGQNVPFVTGSFTSTGTGTTSPFQTIERKDV
                     GITLKIKPQIGENGTVRMTIYQESSSVASTTAVGTDNAGPTTNKRSIESTITVDDGQI
                     IVLGGLIEDSYNTTRSKVPLLGDIPYLGALFRSENRTRKRTNLMVFLRPVVMRDQAGS
                     NKISLDRYDYIRAQQQENQPPQSTILRINESPVLPQIRPGDVGPTGAPRPLGDTAAPP
                     PPLAPSIPASGAAPEPVPASAPKTN"
BASE COUNT          484 a         1199 c         1227 g          572 t
ORIGIN      
        1 acgatcagca gcggctcgag gatggtcgcc atctgcagcg cacggcgctg gacctcggtg
       61 ccgagctggc gcgcagcacg gtccagcatc tgcgggagct ggccggtctg ctcgcccagg
      121 cgcgagaaca tcgcgagcag cccggggaag cgcttcttgc cagccagggc cgatgcgagc
      181 ggagcgcctt cgcgcacctg caccagtgcc tccatcgcat cggcgcgcat cgcgcggttc
      241 gacagcgtct cggccgcggc ctgcaaggcc ttcaggatcg gcacgccggc gccggcgagc
      301 atcgccagcg tgccggcaaa gcgcgccgcg ttgtagccgc gcgcgagctt gccgatcagc
      361 ggcagttcga gccagccggc gtcgaagctc tggcggaaca cctcgttgcg cagcatcgcc
      421 agcagcaggc cgacgccggc ggcgatcaac agcagcacgg cccagcccca attgcgcagg
      481 aacgcgctga ggcccagcat cgccaccgtc agcgcgggca gcgcgcgctt cgaactgctg
      541 aacaccgacg cgacctgcgg cacgacgtag gtgacgagaa agatcacgat cacgatcgcg
      601 atcagcgaga cgatcgccgg gtacagcgtc gcgccgatca gcttgctgcg cagctcctgg
      661 cgttcctcga ggtcgtcggc gaggcgttcg agcactttgc ccagcgcgcc gctctgctcg
      721 cccgcggcga ccacgccgcg atagacctcg tcgaactcgc gcggtgcgct gcccagcgcg
      781 cgtgcgaacg gcgagccgcc gttgacctcg ctcttcaggt gcgcgacgag ctcgcgctgg
      841 cgcgggtctt cggcctcctc gctgagcgcg gtcagcgcgc gttccagcgg cagaccggcg
      901 ccgaccaggc cggcgagctg gcgcgtccag accgtcaggc tggtggtgtt gaagacgcgc
      961 cgcgtgaacc gcaagccttc ggcctgcgtg ccggccgacg ccaccagcgt gaccgccatc
     1021 ggcaccagcg cctgcgcgcg caactggctg cgtgcggcct tggcgttctc ggcgtcgagc
     1081 aggccggtgg tcgacttgcc ggccgcgtcg agcgcctcga atttgtacgc cggcatgggg
     1141 gctattcgcg cgtgacgcgc atcacttctt cgggcgatgt gacgccttcg gcgacgagcc
     1201 gctcgccgtc ctcgcgcatc gagcgcagcc cggccgcttc ggcggcgacg aagatctgcg
     1261 actcggcggc gcggccgtgg atcaacgccc tgacctggtc gtccaccgcc atcaactcgt
     1321 agacgccggt gcggcccttg tagccggtgt tgccgcactc gacgcagccg accgggtggt
     1381 agcgcccgcg tgcgtcgatc ttcttgcacg ccgggcagag cttgcgcacc agccgctgcg
     1441 cgagcacgcc gatcaggctg gagctcagca ggaacggctc gacgcccatg tcgatcatcc
     1501 gcgtcaccgt gctcggcgcg tcgttggtgt ggacggtggc gagcaccagg tggccggtca
     1561 gcgacgcctg gatcgcgatc tgcgcggtct cgtagtcgcg gatctcgccg atcatgatga
     1621 cgtccgggtc ctggcgcagg atcgcgcgca gcgccttcgc gaacgtcagg tcgatcttcg
     1681 gattcacctg ggtctggccg atgcccgcga gttcgtactc gaccgggtcc tcgaccgtca
     1741 gcacgttggt cgtggcggtg tcgatgcggc ccagcgacgc gtacagcgtc gtcgtcttgc
     1801 ccgagccggt cggcccggtc acgagcacga tgccgtgcgg ctgctgcacc agcttgtcga
     1861 agcgctgaag caccgcgccg ctcatgccca ggccttcgag cgtgaacttc gactcgccct
     1921 tgtcgagcag gcgcagcacc gcacgctcgc cgtgcgacga cggcagggtc gagacccgca
     1981 catcgaccgc gcggccgccg atgcgcagcg agatgcggcc gtcctgcggc agacgccgtt
     2041 ccgcaatatc cagctcggcc atgatcttca ggcgcgagat cagcgcggcg tgcagcgcct
     2101 tgttcggctg aaccacttcg cgcaaggtgc cgtcgacgcg gaagcgcacc gagctgctgc
     2161 gctcgtaggg ttcgatgtgg atgtcggagg cgccgtcctt cgcggcctgc gtcaacagcg
     2221 cgttgagcat gcgaatgatc ggcgcgtcgt tgctcgcctc gagcaggtcc tcgatcgccg
     2281 gcagctcctg catcatgcga ctcaggtcga ccgcgctctc gacctcgccg atcacggtgg
     2341 cggcgctcga ttcgctgccc gcgtaggcga ccgcgatgcg gttcgccagc gacgaagccg
     2401 cctcgcgttc gagcgcgtcg acgtcgtaca ggcgcagcac ctcgccgagc gccggcaggc
     2461 cgaccgattc gggcgcccac agcaccagcc gcgcgccgtc gtcctcgagc agcagcgtgt
     2521 gcgccttcgc gtaggcgtag ggcagggggt ggcgggcgcc catggtgctt cgctcctcag
     2581 ttcgtcttcg gcgcggacgc gggcaccggt tctggcgccg cgcccgacgc cggaatcgaa
     2641 ggcgccagcg gcggcggtgg cgccgccgtg tcgcccagcg gccgcggtgc gcccgtcggg
     2701 ccgacatcgc ccggccggat ctgcggcagc accggcgatt cgttgatgcg caggatcgtg
     2761 ctctgcggcg gctggttctc ctgctgctgg gcgcggatgt agtcgtagcg gtcgagcgag
     2821 atcttgttgg acccggcctg atcgcgcatc accaccgggc gcaggaacac catcaggttg
     2881 gtgcgcttgc gggtgcggtt ctcgctgcgg aacagcgcgc cgaggtaggg gatgtcgccg
     2941 agcagcggca ccttcgagcg cgtcgtgttg tagctgtctt cgatcagccc gccgagcacg
     3001 atgatctggc cgtcgtcgac cgtgatggtc gattcgatcg atcgcttgtt ggtcgtcggg
     3061 ccggcgttgt cggtgcccac cgcggtggtc gacgcgaccg acgacgattc ctggtagatc
     3121 gtcatgcgca cggtaccgtt ctcgccgatc tgcggcttga tcttcagcgt gatgccgacg
     3181 tccttgcgct cgatggtctg gaacgggctg gtcgtgccgg tgccggtgct ggtgaacgag
     3241 ccggtgacga acggcacgtt ctggccgacg acgatgcgcg cctcttcgtt gtcgagcgtc
     3301 accaggttcg gtgtcgagag gatgttcgtg ccggtggtcg aactgagggc ttgggccagc
     3361 gcgccgagcg tgtagaagtt gccgatcttg tgcgccaggc cgatgttgaa gcccgcgttc
     3421 ggcaaaccgc tgttcaaggc cgagatcacg ccgctggagc cggtggccag cgccgcgctg
     3481 cc
//
LOCUS       DATMJB010000138         2560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_277638, whole genome shotgun sequence.
ACCESSION   DATMJB010000138 DATMJB010000000
VERSION     DATMJB010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277638"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /gene="lplT"
                     /locus_tag="VNU48_03125"
     CDS             complement(<1..799)
                     /gene="lplT"
                     /locus_tag="VNU48_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HWI10298.1"
                     /translation="MKKGFYTIMSAQFFSSLADNALLVAAIEMLKTTNAPSWNIPALG
                     PMFALFYVILAPFLGAFADAVPKGKVMFLSNGIKVGGCLLMLFGGHPLVAYAIVGLGA
                     AAYSPAKYGILTELLPNSQLVKANGWIEGLTIMSIILGIALGGQLIGVVVSRHLLAVD
                     LPLIDTGISTAPESAISVIVFLYLIAAMFNLRIPRTEAPLQPIAHGMLGLMRDFSSCN
                     ARLWSDKLGQISLATTTLFWGVSGNLRIIVFAWATAALGYTTTQATSL"
     gene            complement(938..2212)
                     /gene="lplT"
                     /locus_tag="VNU48_03130"
     CDS             complement(938..2212)
                     /gene="lplT"
                     /locus_tag="VNU48_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HWI10299.1"
                     /translation="MKKGFATIMAAQFFSALADTALGVVALELLRAENMASRVAWLAP
                     MLALFYVLLAPFLGAFADALPKGKVMLAANGVKMLGCLLMLAGVNPLLAYAVVGLGAA
                     AYSPAKYGIVIELLPASQLVKANGWIEGLTIVSILLGLGCGGALVGPTVSRHLLAFDL
                     PFFDTGLTTAPDSALALLMAVYLIAAMLNLRIPRTDAPLQPIGNDVGALLRDFVDCNA
                     RLWSDKLGQISLATTTLFWGASANLKNIVFAWAAVALGYTTSQTSALVVVLALGTAAG
                     AVAASMRVRLERATDVIPLGLVMGMLVMGLSLIDSPWAAAPFLLVLGGIGGYLVVPMN
                     ALLYHRGANLMGAGRSLAVQNFNEQACILGFAAFYIGTAKLGLSVVGAVALFGLLVTA
                     TMALVVRWHRSNRVRHRAEVDQLLAVARSGSH"
     gene            2354..>2560
                     /locus_tag="VNU48_03135"
     CDS             2354..>2560
                     /locus_tag="VNU48_03135"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWI10300.1"
                     /translation="MPRPVEALIHTEALAHNLARARAAAPDAKVWAVVKANAYGHGIE
                     HAYAGLKGADGFALLDLREAERIRA"
BASE COUNT          451 a          915 c          859 g          335 t
ORIGIN      
        1 ccagcgaggt cgcctgcgtc gtcgtgtagc cgagcgccgc ggtcgcccac gcgaacacga
       61 tgatgcgcag gttgcccgag acgccccaga acagcgtcgt cgtcgccagc gagatctggc
      121 cgagcttgtc ggaccacagc cgcgcgttgc agctcgagaa gtcgcgcatc aggcccagca
      181 tgccgtgggc gatcggctgc agcggcgcct cggtgcgcgg gatgcgcagg ttgaacatcg
      241 ccgcgatcaa gtacaggaac acgatcaccg agatcgcgga ctcgggcgcg gtcgagatgc
      301 cggtgtcgat cagcggcagg tcgaccgcca gcaggtggcg cgacaccacc acgccgatca
      361 gctggccgcc gagcgcgatg cccaggatga tcgacatgat cgtcaggccc tcgatccagc
      421 cgttcgcctt gaccagctgc gagttcggca gcagctcggt caggatgccg tacttcgcgg
      481 gcgagtacgc cgcggcgccg aggccgacga tcgcataggc caccagcggg tggccgccga
      541 acagcatcag caggcagccc cccaccttga tgccgttcga caggaacatc accttgccct
      601 tcggcaccgc gtcggcgaag gcgccgagaa agggcgcgag gatcacgtag aacagcgcga
      661 acatcggccc caatgccggg atgttccacg acggcgcgtt cgtcgtcttc agcatctcga
      721 tcgcggccac cagcagcgcg ttgtcggcca gcgagctgaa gaactgcgcc gacatgatcg
      781 tgtagaagcc cttcttcatg cgcgcagcaa ctcggcttca aactgggggg gtcggcacac
      841 catggcggca ttgtcgccgc agccgcggca cgtttgggcc tgcagccgga cgccgggtta
      901 gcccttagtc ggccgcgcgc agcggcgcgt gtttttctca gtgcgagcca ctgcgcgcga
      961 cggcgagcag ctggtcgact tcggcgcggt gccgcacgcg gttcgagcgg tgccagcgga
     1021 ccaccagcgc catcgtcgcc gtcaccagca acccgaacag cgccacggcg ccgaccaccg
     1081 acaggccgag cttcgccgtg ccgatgtaga acgccgcgaa gccgaggatg caggcctgct
     1141 cgttgaagtt ctgcaccgcg agcgaccggc ccgcgcccat caggttcgcg ccccggtggt
     1201 agagcagcgc gttcatcggc acgaccaggt agccgccgat gccgccgagc acgagcagga
     1261 acggcgccgc cgcccagggg ctgtcgatca ggctcaggcc catcaccagc atacccatca
     1321 cgaggcccag cggaatcacg tcggtggcgc gctccagccg cacccgcatc gacgcggcga
     1381 ccgcgcccgc cgcggtgccc agcgccagca cgaccaccag cgccgaggtc tgcgaggtcg
     1441 tgtagcccag cgccaccgcg gcccaggcga acacgatgtt cttcaggttc gccgatgcgc
     1501 cccagaacag cgtcgtcgtc gccagcgaga tctggccgag cttgtcggac cacaggcgcg
     1561 cgttgcagtc gacgaaatcg cgcagcagcg cgccgacgtc gttgccgatc ggctgcagcg
     1621 gcgcgtcggt gcgcggaatg cgcaggttca gcatcgcggc gatcaggtac acggccatca
     1681 gcagcgccag cgccgagtcc ggcgcggtgg tcagcccggt gtcgaagaac ggcagatcga
     1741 acgcgagcaa atggcgcgac accgtcggcc cgaccagcgc gccgccgcag cccaggccga
     1801 gcaggatcga gacgatcgtc agcccttcga tccagccgtt cgccttgacc agctgcgagg
     1861 cgggcagcag ctcgatcacg atgccgtact tcgcgggcga gtaggccgcc gcgccgaggc
     1921 cgaccaccgc gtaggcgagc agcgggttga cgccggccag catcagcagg cagccgagca
     1981 tcttgacgcc gttcgccgcc agcatcacct tgcccttcgg cagcgcatcg gcgaaggcac
     2041 ccaggaacgg cgcgagcagc acgtagaaca gcgccagcat cggcgccagc caggccacgc
     2101 gcgaggccat gttctcggcg cgcagcaact ccagcgcgac cacgcccagc gcggtgtcgg
     2161 ccaaagcgct gaagaactgc gccgccatga tcgtggcgaa tcccttcttc atcggcccgt
     2221 ccgcgtggcg ccgctcgccc ggtgtttgcg catgagtctc cttggcgaac ggcgttatag
     2281 cacgcaggtt tgacgccgcc gcgactggca gaatcgggcc atgccgcgcg atcccacctg
     2341 aaggacacgg acgatgccgc gccccgtaga agccctgatc cacaccgaag cgctggccca
     2401 caacctggca cgtgcgcgcg ccgcggcgcc cgacgccaag gtctgggcgg tcgtgaaggc
     2461 caacgcctac ggccacggca tcgagcacgc ctatgccggg ctgaagggcg ccgacggttt
     2521 cgcgctgctc gacctgcgcg aggccgagcg catccgcgcg
//
LOCUS       DATMJB010000139         5541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_691829, whole genome shotgun sequence.
ACCESSION   DATMJB010000139 DATMJB010000000
VERSION     DATMJB010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691829"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VNU48_03140"
     CDS             complement(<1..194)
                     /locus_tag="VNU48_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HWI10301.1"
                     /translation="MNEDERMHSPAAERNRGPILGELQRLLPAHGIALEVASGTGQHA
                     AHFAAGLPAWIWQPSDTRPQA"
     gene            complement(191..2119)
                     /locus_tag="VNU48_03145"
     CDS             complement(191..2119)
                     /locus_tag="VNU48_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWI10302.1"
                     /translation="MPVSPTLAPLRVLSIIPPMTQLNTPYPSTAYLTGFLRSRGVAAV
                     QEDLALALVLTLFSADGLQQLRAGIAALPQPTPLVRAFVAQFERYLATITPTIAFLQG
                     RDPTLGHRIAGRAFLPEGARFESLEVYVDDEGGDPLAWAFGALGVQDRARHLATLYLN
                     DIADVLRDAVDPRFEFVRYAESLAQSQPTFEPLAAALAAPLNLVDRTLVALTLDALRR
                     HAPRVVLVSVPFPGSVYAAFRIAQAIKAHDPDIVTVLGGGFVNTELRELTEPRVFDHF
                     DYVTLDAGERPLLALLDHLQGRRSRQRLVRTFVRDAESGAVRYVNLVEPDIAFAEVGT
                     PTWDGLPLERYLSLLDMLNPMHRLWSDGRWNKLTVAQGCYWKKCSFCDVSLDYISRYE
                     GASASLLVDRIEQIVAETGQTGFHFVDEAAPPKALKTLAAELLRRQRSISWWGNIRFE
                     KSFSQQLCEQLAASGCIAISGGLEVASDRLLTLMKKGVSVDQVARVTKAFADAGILVH
                     AYLMYGFPTQTVQDTVDALEYVRQLFEAGCIQSGFFHRFACTVHSPVGQHPADYGVTL
                     MPLPQLPAHTAFAKNDIGFHDPTGTDHDALGAALKKALYNYMHGIGLDEDVRQWFDFH
                     VPKTRVPRHKIERALETP"
     gene            2173..2793
                     /locus_tag="VNU48_03150"
     CDS             2173..2793
                     /locus_tag="VNU48_03150"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWI10303.1"
                     /translation="MSDPTHFYEPRLGHGLPHDPFNAIVGPRPIGWISSIDAQGRLNL
                     APYSFFNGFNYTPPIIGFASVGAKDTLRNVQQTREFAWNLATRPLAEQMNQTCATVPP
                     EVDEFALAGLTPAPSRVIAVPRVAESPVSFECRVTQIVQLQGADGTKVETWLTLGEVV
                     AVHIAKRLLKAGVYDTAAAEPILRGGGPAEYFGIDAAQRFAMRRPR"
     gene            complement(2819..3895)
                     /locus_tag="VNU48_03155"
     CDS             complement(2819..3895)
                     /locus_tag="VNU48_03155"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HWI10304.1"
                     /translation="MSRFRNYWHLHDWRDYRKRRDGELLLRYIGWAGHRNVGDDALYL
                     AFKRQLCGRALLLPIDDDSPLSMRSRHWRSSTVVLGGGTLINVDSYLAPLEHLVAQGE
                     RFVVFGTGVADLDFWSRHPGQGRGQAGRWLRVLRSARHIGVRGPRSLRWLAENGIDHA
                     EVIGDPALSLTTPTEAASARGGAGRVGINLGSHDPVNGGQDRTLQAALALGRHLLSRG
                     LQIRYVSLSDGDHRLGRMMAAQLDHAGFELLPFSIDVDRSLRDIAGCDWLVGQRLHAT
                     VLASALGVPNLSLSYQPKCLDFLESIGCEELSVDTAQINGHTLVERFEDLVAECHHWR
                     TRLLERGGQYRRLQQQRAQAFGWA"
     gene            4030..5076
                     /locus_tag="VNU48_03160"
     CDS             4030..5076
                     /locus_tag="VNU48_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWI10305.1"
                     /translation="MHETSWHDGHAGASAWLATALLIAAVLAGCAAGPPRVVPDRDGP
                     EANPPADLARVPDAEPALEPIRPGGPNKPYDMLGKTYVPITQDAPFTERGLASWYGRK
                     FHGRRTASGEVYNMYAMTAAHPTLPIPSYARIRNPANGREVVVRINDRGPFHSTRIVD
                     LSYTAALKLDLLRGVAPVELQRITSDDIRTGAWRRAPEGDAAQLATRGAIEPAAIAPP
                     LPAASGAATPELLPVSTAGVASATTAATADTPLAAEAEAIADAPAARAFTTPARGFWV
                     QLGAFKRRDGAEGFHQRVAAELDWLSPLLAVFDDAAMFRLQAGPYASREQARSVAQRV
                     RGGLQLVPVIVERR"
     gene            5200..>5541
                     /locus_tag="VNU48_03165"
     CDS             5200..>5541
                     /locus_tag="VNU48_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10306.1"
                     /translation="MSNTAPRAVAIAAVQEVQTADDGAAIIVKAHAQDGDARSPLEIA
                     ITTELAAAVAISLLATTARSRRSRDSMEPALECFAAGVEDSSLHQDRVRLQLLFEGGT
                     VLPVEMGRDAGE"
BASE COUNT          867 a         1948 c         1938 g          788 t
ORIGIN      
        1 gcttgcggac gcgtgtcgct cggctgccag atccaggccg gcaatccagc ggcgaagtgc
       61 gccgcatgct ggccggtgcc gctggcgact tccagcgcga tgccgtgcgc cggcagcagg
      121 cgctgcagtt cgcccaggat cggcccgcgg ttgcgctcgg ccgccgggct gtgcatgcgt
      181 tcgtcttcat tcatggcgtt tccagggcgc gttcgatctt gtgccgcggc acgcgcgtct
      241 tcggcacgtg gaagtcgaac cactggcgca cgtcctcgtc cagcccgatg ccgtgcatgt
      301 agttgtacag cgccttcttc agtgccgcgc cgagcgcgtc gtggtcggtg ccggtcgggt
      361 cgtggaagcc gatgtcgttc ttcgcgaacg cggtgtgcgc aggcagctgc ggcagcggca
      421 tcagggtcac gccgtagtcg gccgggtgtt ggccgaccgg cgaatgcacg gtgcaggcga
      481 aacggtggaa gaagcccgac tggatgcagc cggcctcgaa cagctggcgc acgtactcga
      541 gcgcgtcgac cgtgtcctgc accgtctgcg tcggaaagcc gtacatcagg taggcgtgga
      601 ccaggatgcc ggcgtccgcg aacgccttcg tcacgcgcgc cacctgatcg accgagacgc
      661 ccttcttcat cagcgtcagc aggcggtccg aggcgacctc cagcccgccc gagatcgcga
      721 tgcagccgct cgccgcgagc tgctcgcaca gctgctggct gaacgatttc tcgaaacgga
      781 tgttgcccca ccacgagatc gagcgctggc gtctgagcag ctcggccgcg agcgtcttca
      841 gcgccttcgg cggcgcggcc tcgtcgacga aatgaaagcc ggtctggccg gtctcggcca
      901 cgatctgctc gatccggtcg accagcaacg acgccgacgc accttcgtag cgcgagatgt
      961 agtccaggct cacgtcgcag aagctgcact tcttccagta gcagccctgc gcgacggtca
     1021 gcttgttcca gcgcccgtcg gaccacagcc ggtgcatcgg gttgagcatg tcgagcagcg
     1081 acaggtagcg ctccagcggc aagccgtccc aggtcggcgt gccgacctcg gcgaacgcga
     1141 tgtcgggctc gacgaggttg acgtagcgca ccgcgccact ctcggcatcg cgcacgaagg
     1201 tgcgcaccag gcgctggcgc gagcgccggc cctgcaggtg gtcgagcagc gcgagcagcg
     1261 ggcgctcgcc ggcgtcgagc gtgacgtagt cgaagtggtc gaacacgcgc ggctcggtca
     1321 gctcgcgcag ctcggtgttg acgaagccgc cgccgagcac ggtgacgatg tcggggtcgt
     1381 gggccttgat cgcctgcgcg atccggaacg cggcgtagac cgagccgggg aacggcaccg
     1441 acaccagcac gacccgcggc gcgtggcggc gcagcgcgtc cagcgtcagc gcgaccagcg
     1501 tgcggtcgac caggttcagc ggcgccgcga gcgcggcggc cagcggctcg aaggtcggct
     1561 ggctctgcgc cagcgattcg gcgtagcgca cgaactcgaa ccgggggtcc accgcgtcgc
     1621 gcagcacgtc ggcgatgtcg ttcaggtaca gcgtcgcgag atggcgcgcg cggtcctgca
     1681 cgccgagcgc gccgaaggcc caggcgagcg ggtcgccgcc ttcgtcgtcg acgtagacct
     1741 cgagcgactc gaagcgcgcg ccctcgggca ggaaggcgcg gccggcgatg cggtggccca
     1801 gcgtcgggtc gcggccctgc aggaacgcaa tcgtcggcgt gatcgtcgcg aggtagcgct
     1861 cgaactgcgc gacgaaggcc cgcaccagcg gcgtgggctg cggcaacgcc gcgatgccgg
     1921 cgcgcagctg ctgcaggccg tcggcggaga acaaggtcag cacgagcgcg agcgcgaggt
     1981 cctcctgcac cgcggcgacg ccgcgcgagc gcaggaagcc ggtcaggtac gcggtcgacg
     2041 ggtagggtgt gttcagctgc gtcatcggcg ggatgatcga cagcacacgc agcggggcga
     2101 gcgttgggga aaccggcatg cggcgagtct gccatgcacg gactgcgcgg cccacgtaca
     2161 gtgcgcaaca ccatgagcga cccgacgcac ttctacgaac cgcgcctggg ccacggcctg
     2221 ccgcacgacc cgttcaatgc gatcgtcggc ccgcgcccga tcggctggat ctcatcgatc
     2281 gacgcgcagg ggcgcctgaa cctcgcgccg tacagcttct tcaacggttt caactacacg
     2341 ccgccgatca tcggtttcgc cagcgtcggc gcgaaggaca cgctgcgcaa cgtgcagcag
     2401 acccgcgagt tcgcgtggaa cctggcgacc cggccgctgg ccgagcagat gaaccagacc
     2461 tgcgcgaccg tgccgcccga ggtcgacgag ttcgcgctcg ccggcctgac gcccgcgccc
     2521 tcgcgcgtga tcgcggtgcc gcgcgtggcc gagagtccgg tgtcgttcga atgccgggtg
     2581 acgcagatcg ttcagctgca gggcgccgac ggcaccaagg tcgagacctg gctgacactg
     2641 ggcgaggtcg tcgcggtcca catcgccaaa cgactgctga aggccggcgt ctacgacacc
     2701 gcggcggccg agccgatcct gcgtggcggc ggcccggcgg agtacttcgg catcgacgcg
     2761 gcgcagcgct tcgcgatgcg tcgcccgcgc tgagcgccgt gccgccgcgc gcgcagcatc
     2821 aagcccaacc gaacgcctgc gcccgctgct gctgcaggcg ccggtactgg ccgccgcgct
     2881 cgagcagtcg cgtgcgccag tggtggcact cggccacgag gtcttcgaaa cgctcgacca
     2941 gcgtgtgccc gttgatctgt gcggtgtcga ccgacagctc ctcgcagccg atcgattcga
     3001 gaaagtccag gcacttgggc tgatagctca gcgagaggtt cggcacccca agggcgctgg
     3061 cgagcacggt ggcgtgcagg cgctggccca ccagccaatc gcagccggcg atgtcgcgca
     3121 agctgcggtc gacatcgatc gagaacggca gcagctcgaa gccggcatga tccagttgcg
     3181 ccgccatcat gcggcccagc cgatgatcgc cgtcgctcaa cgacacatag cgaatctgca
     3241 gcccacgcga cagcaggtgg cggccgaggg cgagcgcggc ttgcagggtt cgatcctggc
     3301 caccgttcac gggatcgtgg ctgcccaggt tgatgccgac ccggccggcg ccgccacgcg
     3361 ccgacgccgc ctcggtcggt gtcgtcagcg acagtgccgg atcaccgatg acctcggcgt
     3421 ggtcgatgcc gttctcggcg agccatcgca gcgagcgcgg cccccgcaca ccgatgtgac
     3481 gggccgagcg caaaacgcgc agccaccgac ccgcctggcc gcggccctga cccggatggc
     3541 gcgaccagaa gtcgaggtct gcgacaccgg tgccgaagac cacgaagcgc tcgccctggg
     3601 ccaccaggtg ctccagcggc gcgaggtagg agtcgacgtt gatcagcgtg ccaccgccca
     3661 gcacgaccgt gctcgaccgc caatggcgcg aacgcatcga caacggcgag tcatcgtcga
     3721 tcggcaataa cagtgcacgc ccgcagagct ggcgcttgaa tgccaggtac agcgcgtcgt
     3781 cgccgacatt gcgatgcccg gcccagccga tgtagcgcag cagcaactcg ccatcacggc
     3841 gtttgcggta gtcgcgccag tcgtgcagat gccagtagtt ccggaatcgg ctcaatcgcc
     3901 gccgaatgga cgactccatc gcgaccctct ctgtcttgtg tggtgcatca tcatgcacga
     3961 taggcaaagt tgtcttgtcg agttgtaaac ggactggtcg tatgcatcag tcgtgtatgg
     4021 tcgcgcttca tgcatgagac ctcatggcac gacggccatg ccggcgcgtc ggcgtggctc
     4081 gcaacggccc tgctgatcgc tgcggtgctg gcgggctgcg ccgcaggacc gccgcgggtg
     4141 gtgcccgacc gcgacggccc cgaggcgaac ccgccggccg atctggcgcg cgtgcccgat
     4201 gccgaacccg cgctcgagcc gatccgcccc ggcggcccga acaagcccta cgacatgctc
     4261 ggcaagacct atgtgccgat cacgcaggac gcgccgttca ccgagcgcgg cctggcctcg
     4321 tggtacggcc gcaagttcca cggccggcgc accgccagcg gcgaggtcta caacatgtac
     4381 gcgatgacgg ccgcgcaccc gaccttgccg atcccgagct atgcgcgcat ccgcaacccg
     4441 gcgaacggcc gcgaggtcgt cgtgcgcatc aacgaccgcg ggccgttcca ttcgacccgc
     4501 atcgtcgacc tgagctacac cgcggcgctc aagctcgacc tgctgcgggg cgtcgcgccg
     4561 gtggagctgc agcgcatcac ctctgacgac atccgcaccg gcgcatggcg gcgcgcgccc
     4621 gagggcgacg cggcgcaact cgcgacgcgc ggcgcgatcg agccggccgc gatcgctcct
     4681 ccgctgcctg cggcgtcggg cgcggcaacg cccgagctgc tgccggtgtc gaccgcgggc
     4741 gtggcgtccg cgaccacggc ggccacggcg gacacgccgc tcgcggccga ggccgaggcc
     4801 atcgccgacg cgccggccgc gcgcgcgttc accacgccgg cgcgcggctt ctgggtccag
     4861 ctcggcgcgt tcaagcggcg cgacggcgcc gaaggtttcc accagcgcgt cgcggccgaa
     4921 ctcgactggc tgtcgccgct gctcgcggtc ttcgacgatg ccgcgatgtt ccgtctgcag
     4981 gccggcccgt atgccagccg cgagcaggcg cgcagcgtcg cccagcgggt gcgcggcggc
     5041 ctgcagctgg tgccggtgat tgtcgagcgg cgttgagctc accgtcagac atcccctacc
     5101 gcgcgatgcg ccggccgcgc cttgccggcg gcacgccact tgcgaagaat tcatcaagcg
     5161 ctgccattgg cggcagcccc gaaccgaaca acgaacgtca tgagcaacac cgccccaagg
     5221 gctgtcgcca tcgcggcagt ccaggaagtc cagaccgccg acgacggcgc cgcgatcatc
     5281 gtcaaggcgc atgcgcagga tggtgacgcg cgctcgccgc tcgagatcgc gatcaccacc
     5341 gaactggcgg cggcggtggc gatctcgctg ctcgccacca ccgcgcgttc gcggcgctcg
     5401 cgcgattcga tggagccggc gctcgagtgc ttcgccgcag gcgtcgaaga cagctcgctg
     5461 caccaggacc gggtgcgcct gcaattgctg ttcgagggcg gcaccgtgtt gccggtcgag
     5521 atgggtcgcg atgcgggcga g
//
LOCUS       DATMJB010000140         2506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_218275, whole genome shotgun sequence.
ACCESSION   DATMJB010000140 DATMJB010000000
VERSION     DATMJB010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218275"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VNU48_03170"
     CDS             <1..1198
                     /locus_tag="VNU48_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI10307.1"
                     /translation="AALGLPGAMLRPLSKEFGWSTDQLSSVFAVRFALFGLLGPFAAI
                     FIARFGLRRIMVTAASFIAISMLLATQVTQLWQLFLLWGLVLGCGTGLTALVLGAMVA
                     SRWFTERRGLVVGLFAASTATGQLIFLPVAAWMIEHLGWRYAVIPIFVACALVATLAT
                     LFMHDHPRDVGLRSYGEPEGTPQDVIPATPAPLNFLGPFQVLAQASRNRTFWILAGSF
                     FICGLSTNGLVQTHFISLCGDNGLSAVPAASVLSMMGLFDLFGTTLSGYLSDRYDNRK
                     LLFWYYGLRGLSLFWLPYSTFTLFGLSTFAMFYGLDWVATIPPTVKLAAQEFGKERAG
                     MIFGWIFAAHQLGAAVAAYGAGLTRTLLLTYNPALFAAGAACLLAAVMVLAIRRQAQP
                     VKVAVT"
     gene            complement(1200..1823)
                     /gene="eda"
                     /locus_tag="VNU48_03175"
     CDS             complement(1200..1823)
                     /gene="eda"
                     /locus_tag="VNU48_03175"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - 4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0009084 - glutamine family amino acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HWI10308.1"
                     /translation="MNTLDLVSHGPVIPVIVINRLADAVPMARALVDGGVRVLEVTLR
                     TPIALQCMELIAREVPDAILGAGTVRSAADAQAAKDVGCTFAVSPGYTSAIGQACTEI
                     GLPLLPGTATGSEVMQANADGYFFLKFFPAMQAGGIAMLKALAGPFTDVVFCPTGGIT
                     LASAPEFLALPNVKVCGGSWLTPADAVAAQDWARITQLAREASALRP"
     gene            complement(1874..>2506)
                     /locus_tag="VNU48_03180"
     CDS             complement(1874..>2506)
                     /locus_tag="VNU48_03180"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWI10309.1"
                     /translation="PAPETALDETMLRPVASPFLPDGGMRLVQGNLGRGTFKTSAVDR
                     ERWTIEAPARVFDDQDDVVKAFKAGELERDVVVVVRFQGPRANGMPELHKLTPPLGVL
                     QGRGFKVALVTDGRMSGASGKVPAAIHVSPEAMHGGPLGKVRDGDLLRLDAVAGTLAA
                     LVDATAWSAREQAVLPDAQADVNGHGLGRELFAGMRRNVLSAEQGACTWL"
BASE COUNT          316 a          908 c          864 g          418 t
ORIGIN      
        1 ggccgccctc ggcctgcccg gcgcgatgct gcggccgctg agcaaggaat tcggctggag
       61 caccgaccag ctctcgtcgg tgttcgcggt gcgtttcgcg ctgttcggcc tgctcgggcc
      121 gttcgcggcg atcttcatcg cgcgctttgg cctgcgccgc atcatggtca ccgcggcgag
      181 cttcatcgcg atctcgatgc tgctggccac gcaggtcacg cagctgtggc agctgttcct
      241 gctgtggggc ctggtgctcg gctgcggcac cggcctcacg gcgctggtgc tgggcgcgat
      301 ggtcgcgagc cgctggttca ccgagcgccg cggcctggtc gtcggcctgt tcgcggcgag
      361 cacggccacc gggcagctga tcttcctgcc ggtcgcggcc tggatgatcg agcacctggg
      421 ctggcgctat gcggtgatcc cgatcttcgt cgcctgcgcg ctggtcgcga cactggcgac
      481 cttgttcatg cacgaccacc cgcgcgacgt cggcctgcgc tcctatggcg agcccgaggg
      541 cacgccgcag gacgtgatcc ctgccacgcc cgcgccgctg aacttcctcg gtccgttcca
      601 ggtgctcgcg caggcctcgc gcaaccgcac gttctggatc ctcgccggca gcttcttcat
      661 ctgcgggctg tcgaccaacg ggctggtgca gacgcacttc atctcgctgt gcggcgacaa
      721 cggtctttcg gcggtgccgg cggcgtcggt gctgtcgatg atggggctgt tcgacctgtt
      781 cggcaccacg ctgtcgggct acctctcgga ccgctacgac aaccgcaagc tgctgttctg
      841 gtactacggg ctgcgcgggc tgtcgctgtt ctggctgccg tactcgacct tcacgctgtt
      901 cggcctgtcg accttcgcga tgttctacgg cctggactgg gtcgccacga ttccgcccac
      961 ggtcaagctc gccgcccagg agttcggcaa ggagcgcgcc ggcatgatct tcggctggat
     1021 cttcgccgcc caccagctcg gcgccgcggt cgctgcctat ggcgcgggcc tgacgcgcac
     1081 gctgctgctg acctacaacc cggcgctgtt cgccgccggc gccgcgtgcc tgctggccgc
     1141 ggtgatggtg ctggcgatcc ggcgtcaggc gcagccggtc aaggtcgcgg tcacctgact
     1201 caggggcgca gcgcgctcgc ctcgcgcgcg agttgcgtga tgcgcgccca gtcctgcgcc
     1261 gcgacggcgt cggccggcgt cagccacgag ccgccgcaga ccttcacgtt gggcagcgcg
     1321 agaaactccg gcgcgctggc cagcgtgatg ccgccggtgg ggcagaacac gacatcggtg
     1381 aacggcccgg ccagcgcctt cagcatcgcg atgccgccgg cctgcatcgc cgggaagaac
     1441 ttcaggaaga agtagccgtc ggcattggcc tgcatcacct cgctgccggt cgcggtgccg
     1501 ggcagcagcg gcaggccgat ctcggtgcag gcctggccga tcgcgctcgt gtagccgggg
     1561 ctgaccgcga acgtgcagcc cacgtccttc gcggcctgcg catcggccgc gctgcgcacg
     1621 gtgccggcgc cgaggatcgc gtcgggcacc tcgcgcgcga tgagttccat gcactgcagc
     1681 gcgatcggcg tgcgcagggt cacctcgagc acgcgcacgc cgccgtcgac cagcgcacgc
     1741 gccatcggca ccgcgtcggc gagccggttg atcacgatca ccgggatcac cgggccgtgg
     1801 gagacgaggt ccagggtgtt catcatgaag ccggtgtcct gtgaagtttc atgcgggtta
     1861 cggcgcggcg aaatcaaagc caggtgcagg cgccctgctc ggcgctcagc acgttgcggc
     1921 gcatgccggc gaacagctcg cggccgaggc cgtggccgtt gacatcggcc tgcgcatcgg
     1981 gcaacaccgc ctgttcgcgc gcggaccacg ccgtcgcgtc gaccaatgca gcgagcgtgc
     2041 cagccaccgc gtcgaggcgc agcaggtcgc cgtcgcggac cttgccgagc ggccctccgt
     2101 gcatcgcctc cgggctgacg tggatcgccg ccggcacctt gcccgacgcg ccgctcatgc
     2161 gcccgtcggt gaccagcgcg accttgaagc cgcgcccctg cagcacgccc agcggcggcg
     2221 tcagcttgtg cagctcgggc atgccgttgg cgcgcggccc ctggaagcgg acgacgacga
     2281 ccacgtcgcg ctccagttcg cccgccttga acgccttcac cacatcgtcc tgatcgtcga
     2341 acacgcgcgc gggcgcctcg atcgtccagc gctcgcgatc gaccgcgctc gtcttgaacg
     2401 tgccgcggcc gagattgccc tgcaccagcc gcatgccgcc gtcaggcagg aacgggctcg
     2461 cgaccggccg caacatcgtt tcgtcgagcg cggtctcggg tgccgg
//
LOCUS       DATMJB010000141         2515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_751253, whole genome shotgun sequence.
ACCESSION   DATMJB010000141 DATMJB010000000
VERSION     DATMJB010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751253"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2515
                     /locus_tag="VNU48_03185"
     CDS             <1..>2515
                     /locus_tag="VNU48_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10310.1"
                     /translation="LNERIDRITGEFEQRSSTIVDGISDRTEQVHDSLKNSSDSLLLE
                     LELRSGDLVSKIDDAGNRLANRILSSGDKASDALDVTVNTLVAKVISQTENAHDSLAL
                     QMSAFDELVKNQGGELAEKFARDSGTLGALITRHISEFDRTVKTFGGEIVERMGARTQ
                     EISDSLKNYVDTFDTRLVANSGEITASLDQRLLQFETTLGTRVNHLDTSLDSKLRSFD
                     ETIDGRLKSLETTFDTRAKSVTETIDNRLGTLTGSLTDGAAQAIQSIDQRLTLLTSSL
                     TEGTAQAIAAVDHRIANVTETIDGRSAYLTDTIQARFQEIHQGLETRVGNIASGIEIR
                     VGDLASGIETRVGAIAGDIDTRVAQFEDLLGSRIEAVAGRIESSGRQASEDLMSRAEM
                     LSSGIKSHVEDAERSLTNLVVNTAETIQTGARTAQQALLTVSSDVGAQLKLTSAEVEA
                     ALIAVGTGAASSIVTSAQEAQTTLVSASSEAALQIKSLAADVERTLSASGDATAASVL
                     AGAREAQTTLVTASADAANHVKSLAADVERSLSIAGTATAEAVTAGAREAQSTLVAAS
                     TEAATQVRSLSADVQRSLSIAGTATAEAITAGAREAQSTLMSASADASSQVKSLAADV
                     ERSLSMAGNATAESITTGAREAQNTLITASTEAASHVKSLAIDVERTLTAVGAATATS
                     ILGTAREAQSSLSATSADAANQIKAIAADMERSLGVVTASTTDTIQTTAQNAQSALIA
                     ATNEVSTKVKSTSADIERSVLAASSNFGSTMTGKTDEIVTYVQQQTDRLAQIVDSRRG
                     SLVEALSTKATQLTTDIDRVTADALKSIESRGQAFSQSMS"
BASE COUNT          498 a          929 c          748 g          340 t
ORIGIN      
        1 tgctgaacga acgcatcgac cgcatcaccg gcgaattcga gcagcgctcc tcgaccatcg
       61 tcgacggcat ctccgaccgc accgagcagg tccacgactc cctgaagaat tcctccgact
      121 cgctgctgct cgaactcgaa ctgcggtcgg gcgatctcgt cagcaagatc gacgatgccg
      181 gaaaccgcct ggcaaaccgt atcctcagct ccggcgacaa ggccagcgac gcgctcgacg
      241 tgaccgtgaa cacgctggtc gcgaaggtga tcagccagac cgaaaacgcg catgacagcc
      301 tcgcgctgca gatgagcgcc ttcgacgagt tggtgaagaa ccagggcggc gagcttgcgg
      361 aaaaattcgc ccgcgacagc ggcacgctcg gcgcgctgat cacgcgccat atctccgagt
      421 tcgatcgcac cgtcaaaacc ttcggcggcg agatcgtcga gcgcatgggc gcgcgcaccc
      481 aggagatcag cgacagcctg aagaattacg tcgacacttt cgacacccgc ctggtcgcga
      541 acagcggcga gatcaccgct tcgctcgatc agcgcctgct gcagttcgaa accacgctcg
      601 gcacccgcgt caatcacctc gacacttcgc tcgacagcaa gctcaggtcg ttcgacgaga
      661 cgatcgacgg ccgcctgaaa tcgctggaga cgaccttcga tacccgcgcc aaatccgtca
      721 ccgaaaccat cgacaaccgc ctcggcacgc tgacgggttc gctgaccgac ggcgcggcgc
      781 aggcgatcca gtcgatcgac cagcggctta ccctcctcac ctcttcgctg accgagggca
      841 ccgcgcaggc gattgcagcg gtcgaccatc gtattgccaa cgtcaccgaa accatcgacg
      901 gccgcagcgc ctatctgacc gacaccatcc aggcgcggtt ccaggaaatt caccagggcc
      961 ttgagacgcg ggtcggcaac atcgcaagtg gaatcgagat ccgcgtcggc gacctcgcca
     1021 gcggcatcga gacccgggtc ggcgccatcg ccggcgacat cgacacccgc gtggcgcagt
     1081 tcgaggatct gctcggctcg cgcatcgagg ccgtggccgg ccggatcgaa agcagcggcc
     1141 gccaggctag cgaagacctg atgtcgcgcg ccgagatgct gtcctccggc atcaagtcgc
     1201 atgtcgagga cgccgagcgt tcgctgacca atcttgtcgt caacaccgca gagaccatcc
     1261 agaccggcgc gcgcaccgcg cagcaggcgc tgctgacggt ttcctccgat gtcggcgcac
     1321 aactcaagct gacctcggcc gaggtcgaag ccgccctcat cgcggtcggc accggcgcgg
     1381 caagctcgat cgtgaccagc gcccaggagg cccagaccac gctggtgtcg gcctcctccg
     1441 aggcagccct gcagatcaaa tctctcgctg ccgacgtcga gcgcaccctc tcggcctccg
     1501 gcgacgccac ggccgcgtcg gttctcgccg gcgcgcgcga agcgcagacc acgctggtga
     1561 ccgcatccgc agacgcggca aaccacgtca agtcgctggc cgccgacgtc gagcgttcgc
     1621 tgtcgatcgc aggaacagca accgccgagg cggtcaccgc cggcgcccgc gaggcacaga
     1681 gcacgctggt cgccgcctct accgaggccg ccactcaggt caggtcgctc tctgccgacg
     1741 ttcagcgttc gctctcgatc gccggaacgg cgaccgccga ggcgatcacg gcaggcgccc
     1801 gcgaggccca gagcaccctc atgagcgcgt ccgccgacgc atcgagccag gtcaagtcgc
     1861 tcgcggccga cgtggaacgc tcgctctcga tggccggcaa tgccaccgcc gagtcgatca
     1921 cgacgggcgc gcgcgaagcc cagaacaccc tgatcacggc gtcgaccgaa gccgccagcc
     1981 acgtcaagtc gctggcgatc gacgtcgaac gcacgctgac cgcggtcggc gcggccaccg
     2041 ccacctccat cctcggcacc gcgcgcgaag cccagagctc gctctctgcg acctccgccg
     2101 acgccgcgaa ccagatcaag gcaatcgccg ccgacatgga gcgttcgctc ggcgtcgtca
     2161 ccgccagcac gaccgatacc atccagacca ccgcgcagaa cgcacagagc gccctgatcg
     2221 ccgccaccaa cgaggtcagc accaaggtca aatcgacctc ggccgacatc gagcgctcgg
     2281 tgctcgccgc gagcagcaat ttcggctcga cgatgaccgg caagaccgac gaaatcgtca
     2341 cctacgttca gcagcagacc gaccgtctgg cgcagatcgt cgacagccgc cgcggttcgc
     2401 tggtcgaggc gctcagcacc aaggccacgc aactgacgac cgatatcgac cgcgtcaccg
     2461 ccgacgccct gaaatcgatc gagagccgcg gccaggcctt ctcgcaatcg atgtc
//
LOCUS       DATMJB010000142         3662 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_217987, whole genome shotgun sequence.
ACCESSION   DATMJB010000142 DATMJB010000000
VERSION     DATMJB010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217987"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="VNU48_03190"
     CDS             complement(<1..1167)
                     /locus_tag="VNU48_03190"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HWI10311.1"
                     /translation="MSSVGHVIPQAMHFAQPLPLRSGARLRDYTLAYETYGTLNAARS
                     NAVLVCHALNASHHVAGTYADPDGATIKGSEGWWDNLIGPGKPLDTNRFFVIGVNNLG
                     SCFGSTGPMQTSPDTGRPYGADFPVVTVEDWVDAQARLLDALGVEQLAAAIGGSLGGM
                     QALSWSLRYPARLRHCIAVATAPNLSAQNIAFNEVARRAIVTDPEFYGGHFYEHGTLP
                     RRGLRVARMIGHITYLSDDAMEAKFGRELKNGTLGYSTQAVEFEIESYLRHQGDKFSE
                     YFDANTYLLITRALDYFDPAREFGGDLTAAFAAAACKFQIISFTTDWRFAPARSREIV
                     KALVDHKRDVSYAEIDAPHGHDAFLLDDPRYHGILRAYFERVALEVGALRRNHDH"
     regulatory      complement(1216..1290)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            1423..>3662
                     /locus_tag="VNU48_03195"
     CDS             1423..>3662
                     /locus_tag="VNU48_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3772 domain-containing protein"
                     /protein_id="HWI10312.1"
                     /translation="MPIADLHCASSARRARRWLLCAALALPYFAHAQTDDADAALDRI
                     RQQIDTAQQALKTNSDALSDAELQALRSGALAAQSQSSDIAGRLAPQLAGVDARIVEL
                     GTPTPGAKEDADVTAQRALLAKNHTALDGQLKLARLLAVEGEQLAAQAWALRRSQFQA
                     RLGERTNSILAAPFWSELRDDLPRDAQRLAALGSELDRAARATPAAVWLITPFALAAA
                     GWVLWWLQRRAMGWLTTRAPAGRARRSLHALLLVLQWTASFGVAAIALQAVLDWNGTL
                     AAATAALLDRFAGMLWFGGFLFGLANALVCAGRPSWRLLPLPDAVATALRWFPLLLTL
                     VSLASWLAERLAALVNASLSLTVALNCVVALATGLTVVLALMRGERVWRLELAKEGAH
                     ARPLWLTVVAALNWLVLAAAVVCLLAGYVAFGSFAVKQVVWAGLVLGTAYLLAALIDD
                     LLTAWFVTSHAPEKADAPTAARASAQIAVLCSGALRLALLLLAVMLLLAPFGEGPADL
                     LRRSGQWQQGLAVGEFRLQPTAALQALLMLMLGLGAVRLLRRWLAERYLPTTALDPGM
                     RTSATSLFGYAGVVVAVALALSALGIGLERIAWVASALSVGIGFGLQAVVQNFVSGLI
                     LLAERPVKVGDWVSLGGIEGDIRRINVRATEIQMGDRSTVIVPNSEFITKVVRNVTMA
                     EPLGLVQIKLPMPIATDAEQVRTLMLEAYRAHEDVLDTPAPNVLLDGIDGANLLFNAT
                     GFVSSPR"
BASE COUNT          466 a         1276 c         1355 g          565 t
ORIGIN      
        1 gtggtcgtga tttcggcgca aggcgcctac ttcgagcgcg acgcgctcga agtaggcgcg
       61 caagatgccg tgatagcgcg ggtcgtcgag caggaacgcg tcgtggccgt gcggcgcgtc
      121 gatctcggcg tagctcacgt cgcgcttgtg gtcgaccagc gccttgacga tctcgcgcga
      181 gcgcgccggg gcgaagcgcc agtcggtcgt gaagctgatg atctggaact tgcatgccgc
      241 ggcggcgaac gcggccgtca ggtcgccgcc gaactcgcgc gccgggtcga agtagtcgag
      301 cgcgcgcgtg atcagcagat aggtgttggc gtcgaagtac tcgctgaact tgtcgccctg
      361 gtggcgcagg tagctctcga tctcgaactc gaccgcctgg gtgctgtagc cgagcgtgcc
      421 gttcttcagc tcgcggccga acttggcctc catcgcgtcg tcgctcaggt aggtgatgtg
      481 gccgatcatc cgcgccacgc gcaggccgcg gcgcggcagc gtgccgtgct cgtagaagtg
      541 gccgccgtaa aactcggggt cggtgacgat cgcgcgccgt gccacttcgt tgaacgcgat
      601 gttctgcgcc gacaggttcg gcgcggtcgc caccgcgatg cagtggcgca gccgcgccgg
      661 gtagcgcagc gaccagctca gcgcctgcat gccgcccagg ctgccgccga tcgccgcggc
      721 gagctgctcg actcccagcg catcgagcag gcgggcctgc gcgtcgaccc agtcctcgac
      781 cgtgacgacc ggaaaatcgg cgccgtaggg ccggccggtg tcggggctgg tctgcatcgg
      841 cccggtcgag ccgaagcacg agccgaggtt gttgacgccg atcacgaaga agcggttcgt
      901 gtcgagcggc ttgccggggc cgatcaggtt gtcccaccag ccttcgctgc ccttgatcgt
      961 ggccccgtcc ggatcggcgt aggtgccggc gacgtggtgg ctggcgttca gcgcgtggca
     1021 caccagcacc gcgttgctgc gcgcggcgtt cagcgtgccg taggtttcgt aggccagcgt
     1081 gtagtcgcgc agccgcgcgc cgctgcgcag cggcaagggc tgcgcgaagt gcatcgcttg
     1141 gggaatgacg tgaccgaccg aactcatgcg cgcccaacaa aggaaaaccc ggcgccgcca
     1201 aacaagcggg acaccgggtc gtcgggtcca actctttagc ggaatttata gagcgcccgc
     1261 aagccggaac aaatcagcgc tggccggcag tatagcggcc cggcgcggct cgccgcggct
     1321 gcggcagctg cctcgagcca tcgtccgaca gcagggcccg cgcattggca ggtataaccg
     1381 gtcgcatcgc gactcgcccg ccgtgcaggc accgtccccg ccatgccgat cgccgacctt
     1441 cattgcgctt cgtccgcgcg ccgcgcgcgc cggtggctgc tgtgcgcagc gctcgcgctg
     1501 ccgtatttcg cgcacgcgca gaccgatgac gccgatgccg cgctcgaccg gatccgccag
     1561 cagatcgaca ccgcgcagca ggcgctgaag acgaacagcg acgcgctcag cgacgccgag
     1621 ctgcaggccc tgcgcagcgg ggcgctggcc gcgcagtcgc agtcgtccga catcgccggg
     1681 cggctcgcgc cgcagctcgc cggcgtcgac gcgcgcatcg tcgagctcgg cacgccgaca
     1741 ccgggcgcga aggaagacgc ggacgtcacc gcgcaacgcg cgctgctggc gaagaaccac
     1801 accgcgctcg atggccagct caagctcgcg cggctgctcg cggtcgaggg cgaacagctc
     1861 gcggcgcagg cctgggcgct gcggcgctcg cagttccagg cgcggctggg cgagcgcacg
     1921 aactcgatcc tcgcggcgcc gttctggtcg gagctgcgcg acgacctgcc gcgcgacgca
     1981 cagcgcctgg ccgcgctcgg cagcgagctc gatcgcgcgg cgcgcgcgac cccggccgcg
     2041 gtctggctga tcacgccgtt cgcgctcgcc gccgccggct gggtgctgtg gtggctgcag
     2101 cggcgcgcga tgggctggct cacgacgcgc gcgccggccg ggcgagcgcg tcgctcgttg
     2161 cacgcgctgc tgctggtgct gcagtggacc gcgagcttcg gcgtcgccgc gatcgcgctg
     2221 caggccgtgc tcgactggaa cggcacgctg gccgcggcga ccgcggcgct gctcgaccgg
     2281 ttcgccggca tgctctggtt cggcggcttc ctgttcggcc tggcgaacgc gctggtctgc
     2341 gcgggccggc cgtcgtggcg gctgctgccg ctgcccgatg cggtcgcaac ggcgctgcgc
     2401 tggttcccgc tgctgctgac gctggtcagc ctggcgagct ggctggccga gcggctcgcg
     2461 gcgctggtca acgcgagcct gtcgctgacg gtggcgctga actgcgtggt cgcgctcgcg
     2521 accggcctga cggtcgtgct ggcgctgatg cgcggcgagc gcgtctggcg cctcgaactc
     2581 gcgaaggaag gggcgcacgc gcgcccgctg tggctcacgg tcgtcgccgc gctgaactgg
     2641 ctcgtgctgg ccgccgcggt cgtgtgcctg ctcgccggct atgtcgcgtt cggcagcttc
     2701 gcggtcaagc aggtggtctg ggccggcctc gtgctcggca ccgcctacct gctcgcggcc
     2761 ttgatcgacg acctgctgac ggcctggttc gtcaccagcc acgcgcccga gaaggccgac
     2821 gcgccgacgg ccgcccgcgc ctcggcgcag atcgcggtgc tgtgctcggg cgcgctgcgg
     2881 ctggcgctgc tgctgctcgc cgtgatgctg ctgctcgcac cgttcggcga gggcccggcc
     2941 gacctgctgc ggcgcagcgg ccagtggcag cagggcctgg cggtcggcga gttccggctg
     3001 cagccgaccg ccgcgctgca ggcgctgctg atgctcatgc tcgggctcgg cgcggtgcgc
     3061 ctgctgcggc gctggctcgc cgagcgctac ctgccgacga ccgcgctcga ccccggcatg
     3121 cgcacgtcgg cgacctcgct gttcggctat gccggcgtcg tcgtcgcggt cgcgctggcg
     3181 ctgtcggcgc tgggcatcgg gctggagcgc atcgcctggg tcgccagcgc gctgtcggtg
     3241 ggcatcggct tcgggctgca ggcggtggtg cagaacttcg tctcggggct gatcctgctg
     3301 gccgagcggc cggtcaaggt cggcgactgg gtctcgctgg gcggcatcga gggcgacatc
     3361 cggcgcatca acgtgcgcgc caccgagatc cagatgggcg accgctcgac cgtgatcgtg
     3421 ccgaactcgg agttcatcac gaaggtcgtg cgcaacgtga cgatggccga gccgctgggc
     3481 ctggtgcaga tcaagctgcc gatgccgatc gcgaccgatg ccgagcaggt gcgcacgctg
     3541 atgctcgagg cctaccgggc tcacgaggac gtgctcgaca cgccggcgcc gaacgtgctg
     3601 ctagacggca tcgacggcgc gaacctgctg ttcaacgcga ccggcttcgt gagttcgccg
     3661 cg
//
LOCUS       DATMJB010000143         2691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_276966, whole genome shotgun sequence.
ACCESSION   DATMJB010000143 DATMJB010000000
VERSION     DATMJB010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276966"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="VNU48_03200"
     CDS             <1..704
                     /locus_tag="VNU48_03200"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI10313.1"
                     /translation="SEFSSDLAHDLRTPINNLLGEAQVALAKPRCADEYRAVIESAIE
                     DYERISRLIENMLFLARADDPQAATNRRWIDLQPMLERGRGYFEMLAEEREVTLAIEM
                     QGAEPAWQQVWADETMLIRALGNLISNALRYAPRGGRVVVTTTPHDGGGCTLEVTNDG
                     PPIAPEHQQRIFERSFRVDESRAGSATGSGLGLAIVRSIMDLHGGTVGVISGEGRPTM
                     FRLWFPGPVAPGVRV"
     gene            745..1137
                     /locus_tag="VNU48_03205"
     CDS             745..1137
                     /locus_tag="VNU48_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10314.1"
                     /translation="MIQQPADKVEVTVEEVRDSNPSTSAGDASVVIETASLVDGDRRE
                     TSIEVRETDAPAVAAALLNADAATPPAAADLPVAVQCMAAGVVHAASEGRVRLHLQFE
                     SGQVLPIEMPHDAAAALARTLAMHTGVR"
     gene            complement(1159..>2691)
                     /gene="ureC"
                     /locus_tag="VNU48_03210"
     CDS             complement(1159..>2691)
                     /gene="ureC"
                     /locus_tag="VNU48_03210"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HWI10315.1"
                     /translation="GGRAGAEVADTVITNALVIDHWGIVKADIGLKGGLIARLGKAGN
                     PDVQPGVDIVIGPGTEIIAGEGAIVTAGGIDSHIHFICPQQIEEALMNGITTMLGGGT
                     GPATGTFATTCTPGPWHIHAMLKAAESFPMNLGFQGKGNASLPRALEEQVEAGAYGLK
                     LHEDWGTTPAAIDCCLGVADAHDVQVTIHTDTLNESGFVEDTIAAFKGRTIATFHTEG
                     AGGGHAPDIIKCAGLPNVLPSSTNPTMPYTVNTVDEHLDMLMVCHHLDPAIAEDLAFA
                     ESRIRRETIAAEDILHDLGVFSMMSSDSQAMGRVGEVVIRTWQTAHKMKSQRGTLPED
                     NARHDHFRVKRYIAKYTINPAITQGISHVVGSVEVGKLADLVLWKPAFFGVKPSLILK
                     GGMIAAAAMGDPNASIPTPQPVHYRPMFGAFGAAMDNTCLTFISKAAVDLGVPARLGL
                     RRRTEAVKNTRAIGKKDMVHNAGTPHIEVDAQTYEVRADGVLLSCEPATELPMAQRYF
                     LF"
BASE COUNT          379 a          919 c          961 g          432 t
ORIGIN      
        1 tcagcgagtt ctcgtccgac ctggcgcacg acctgcgcac gccgatcaac aacctgctcg
       61 gcgaagcgca ggtcgcgctc gccaagccgc gctgcgccga cgagtaccgc gcggtgatcg
      121 agtcggcgat cgaggactac gagcgcatct cgcggctgat cgagaacatg ctgttcctcg
      181 cccgcgccga cgacccgcag gccgcgacga accgccgctg gatcgacctg cagccgatgc
      241 tcgaacgcgg gcgcggctac ttcgagatgc tggccgagga gcgcgaggtg acgctggcga
      301 tcgagatgca gggcgccgag ccggcgtggc agcaggtctg ggccgacgag acgatgttga
      361 tccgcgcgct cggcaacctg atctcgaacg cgttgcgcta tgcgccgcgc ggcggtcgcg
      421 tcgtcgtcac gacgacgccg cacgacggcg gcggctgcac actcgaggtc acgaacgacg
      481 ggccgccgat cgcgcccgag caccagcagc gcatcttcga acgcagcttt cgcgtcgacg
      541 agtcgcgcgc cgggtcggcc accggttccg gactcggcct ggcgatcgtc cgctccatca
      601 tggacctgca cggcggtacc gtgggcgtga tcagcggcga gggtcggccc accatgttcc
      661 ggctctggtt cccggggccc gtggcgccgg gcgtgcgcgt ctagaatcgg cgcggttcgc
      721 acgccttcgc caaaggacgc cgtcatgatt cagcagcccg ccgacaaggt cgaggtgacc
      781 gtcgaggaag tgagggactc gaatccgagc acgagcgcgg gcgacgccag cgtcgtgatc
      841 gagaccgcat cgctggtcga cggcgaccgg cgcgagacct cgatcgaggt gcgcgagacc
      901 gatgcgccgg cggtcgccgc ggcgctgctg aacgccgacg ccgcgacacc gccggccgcc
      961 gccgatctgc cggtcgcggt gcagtgcatg gccgccggcg tcgtccacgc cgccagcgag
     1021 ggccgcgtgc gcctgcacct gcagttcgaa tcgggtcagg tgctgccgat cgagatgccg
     1081 cacgacgcgg ccgccgcgct cgcgcgcacg ctggcgatgc acaccggcgt gcgctgaccg
     1141 gcgcggcctc agcgcgggtc agaacaggaa gtagcgctgc gccatcggca gttcggtcgc
     1201 cggctcgcag ctgagcagca cgccgtcggc gcgcacctcg taggtctgcg cgtcgacctc
     1261 gatgtgcggc gtgccggcgt tgtggaccat gtccttcttg ccgatggcgc gcgtgttctt
     1321 cacagcctcg gtgcggcggc gcaggccgag ccgggccggc acgccgaggt cgaccgcggc
     1381 cttcgagatg aaggtcaggc aggtgttgtc catcgctgcg ccgaaggcgc cgaacatcgg
     1441 ccggtagtgc accggctgcg gcgtcgggat gctcgcgttc gggtcaccca tcgcggccgc
     1501 ggcgatcatg ccgcccttca ggatcaggct cggcttgacg ccgaagaacg ccggcttcca
     1561 gagcaccagg tcggcgagct tgccgacctc gaccgagccg acgacatgcg agatgccctg
     1621 cgtgatcgcc gggttgatcg tgtacttcgc gatgtagcgc ttgacgcgga agtggtcgtg
     1681 gcgcgcgttg tcttccggga gcgtgccgcg ctgtgacttc atcttgtgcg cggtctgcca
     1741 ggtgcggatg acgacctcgc cgacgcggcc catcgcctgg ctgtccgacg acatcatcga
     1801 gaacacgccc aggtcgtgca ggatgtcctc ggccgcgatc gtctcgcggc ggatgcggct
     1861 ctcggcgaac gcgaggtctt cggcgatcgc cgggtcgagg tggtggcaca ccatcagcat
     1921 gtcgaggtgc tcgtccaccg tgttcacggt gtacggcatc gtcgggttcg tcgacgacgg
     1981 cagcacattg ggcaggccgg cgcacttgat gatgtcgggc gcgtggccgc cgcccgcgcc
     2041 ctcggtgtgg aaggtcgcga tcgttcggcc cttgaacgcc gcgatcgtgt cttcgacgaa
     2101 gccggattcg ttcagcgtgt cggtgtggat cgtgacctgc acgtcatgcg cgtcggccac
     2161 gccgaggcag caatcgatcg ccgcgggcgt cgtgccccag tcctcgtgca gcttcaggcc
     2221 gtaggcgccg gcctcgacct gttcttccaa ggcgcgcggc agcgaggcgt tgcccttgcc
     2281 ctgaaagccc aggttcatcg ggaagctctc ggcggccttg agcattgcat ggatgtgcca
     2341 cggccccggc gtgcaggtgg tcgcgaaggt gccggtggcc gggccggtgc cgccgcccag
     2401 catcgtcgtg atgccgttca tcagcgcctc ttcgatctgc tgcgggcaga tgaagtggat
     2461 gtggctgtcg atgccgccgg cggtcacgat cgcgccctcg ccggcgatga tctcggtgcc
     2521 cgggccgatc acgatgtcca cgcccggctg cacgtcgggg ttgccggcct tgccgaggcg
     2581 cgcgatcagg ccacccttca agccgatgtc ggccttcaca atgccccagt ggtcgatgac
     2641 gagcgcgttc gtgatcacgg tgtcggcgac ctcggcgccg gcccggccac c
//
LOCUS       DATMJB010000144         5818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_632105, whole genome shotgun sequence.
ACCESSION   DATMJB010000144 DATMJB010000000
VERSION     DATMJB010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632105"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VNU48_03215"
     CDS             <1..618
                     /locus_tag="VNU48_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWI10316.1"
                     /translation="LVEPLDLAVVIKTIQRLAPADSILTNGAGNYSGWLHRYYRYPSL
                     QHGGRTQLAPTAGAMGYGVPAAVAAALLHPDRTVINLAGDGDFLMTGQELATATGYGA
                     GTGPGRLIVIVVDNGSYGTIRMHQERVYPGRVSGSDLFNPDFAALARAHRWNAAVVDA
                     TAQFEPALAAALSSDRPTLIHVKLDPDISTSRTTLTAIRRVARER"
     gene            664..1167
                     /gene="rraA"
                     /locus_tag="VNU48_03220"
     CDS             664..1167
                     /gene="rraA"
                     /locus_tag="VNU48_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HWI10317.1"
                     /translation="MSTDSRLVTGDLCDAHKADSDGGFRVLPPVFRDYGQRVRFAGTV
                     STVQCFEDNSFVKAALEAPGQGRVLVVDGGASLRRALVGGNIAAAAARNGWAGVVIDG
                     CVRDSAELAQCDLGIRALALVPMPTERRNAGQRDVPLQIRGVRVQPGDWLVADEDGIV
                     VMPAAPV"
     gene            complement(1211..2953)
                     /locus_tag="VNU48_03225"
     CDS             complement(1211..2953)
                     /locus_tag="VNU48_03225"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWI10318.1"
                     /translation="MQRKLAAILSADAAGFSRLMGDDELATVRALTLCRSLVSDIIVA
                     HHGRVVDSPGDNMLAEFGSAVDAVEAACAMQIQLRSCNADAPEHRRMNFRIGVNLGDV
                     LAEDGRIYGDGVNIAARLEALADAGGICVSGKVHDEVCRKLDCHFADMGLHELHNIAA
                     PVRVYKIVDAAAGAAAAAQAAGAASPRPTGASIIVLPFVNMSGVAEQEFFVDGLTEDI
                     LTELSRFRELFVISRNTSFKFKGQAVDVKKVARDLGVQYVLEGSVRRAGERIRITVQL
                     IDAEADRHVWAERYDRQLEDIFAIQDEMTSAIVAVLPGRVEAATRERAARKPTESMAA
                     YECVVTAKVLHHRSTLLDNTEALRLITRATELDPNYAHAHAWRGCILGQAWTYGWCTD
                     LAAQQVEIHRELTLALGLDDNDSDVHRILAAVFLIQDNFDNAVYHQQRALSLNPNDDL
                     IVVQQGEILTWLGRADEGVEWILKAMRLNPFHPDRFWSHLARAHFVARRYAQAVECLQ
                     HMTKLGDVQHAFLAACLARVGDAQAAALHAGEALRLRPALRVDADYLPMLHYKRADDL
                     AHHRDSLLAAGLPA"
     gene            complement(3030..3743)
                     /gene="serB"
                     /locus_tag="VNU48_03230"
     CDS             complement(3030..3743)
                     /gene="serB"
                     /locus_tag="VNU48_03230"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HWI10319.1"
                     /translation="MTAHESAPGLFIRGFAPPLRLTDYKLVAFDMDSTLINIECVDEI
                     AAVAGRKAEVAAITEAAMRGEIADYKDSLRRRVALLAGVPESALHEVYANHLQLNPGV
                     EPFVQACRAAGLKTLLVSGGFTFFSERVRARLGLDFARANELEIEAGALTGRLVDRPW
                     GDIVDGAEKKRVLLEVCELMGVEPRQAIAVGDGANDLPMMGVAGLSIAYHPKPAVQAQ
                     AMVAILQGGMDRALELFGL"
     gene            complement(3828..>5818)
                     /gene="mfd"
                     /locus_tag="VNU48_03235"
     CDS             complement(3828..>5818)
                     /gene="mfd"
                     /locus_tag="VNU48_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWI10320.1"
                     /translation="RYVGLRNIDLGNDEGGPSEFLHLEYADKATLYVPVAQLHLIARY
                     TGVSADEAPLHRLGSGQWDKAKRKAAEQVRDTAAELLNLYARRAAREGFAFRFQPHDY
                     EAFAASFGFEETPDQQAAIHAVIQDMVSPQPMDRLICGDVGFGKTEVALRAAFIAVTG
                     GKQVAILAPTTLLAEQHYQNISDRFGKWPVRVAEISRFRSSKEVKTTLEGLADGTVDI
                     VVGTHALIGDKVRFRRLGLLVIDEEHRFGVRHKEAIKAMRAEVDVLTLTATPIPRTLG
                     MALEGLRDLSVIATAPQRRLAIKTFVRTETSGTIREAVLRELKRGGQVYFLHNEVETI
                     QARHDKLVELLPEARIAIAHGQMPERELERVMRDFVAQRHNLLLCSTIIETGIDVPSA
                     NTIVMSRADKFGLAQLHQLRGRVGRSHHQAYAYLMVPDVEGLTKAASMRLEAIQSMEE
                     LGSGFYLAMHDLEIRGAGEVLGDHQSGNMMEVGFQLYNEMLAEAVRSLKMGVEPDLLS
                     PLSATTEINLHAPALLPDAYCGDVHTRLSLYKRLASANTSEQIDAMLEEVTDRFGKLP
                     PQGQTLFDVHRLRVLAKPFGVTKIDAAPLLTVISFRPNPPVDALRIIELVQKNRHIKL
                     AGNDKLRIERDTPEPKDRAQLVRDVLRALGTPKQALTA"
BASE COUNT          826 a         1982 c         2076 g          934 t
ORIGIN      
        1 ttggtcgagc cgctggacct cgcggtggtg atcaagacca tccagcgcct cgcgccggcc
       61 gacagcatcc tgaccaacgg cgccggcaac tacagcggct ggctgcaccg ctactaccgc
      121 tacccgtcgc tgcagcacgg cggccgcacc cagctcgcgc cgaccgccgg cgcgatgggc
      181 tacggcgtgc cggcggcggt ggccgccgcg ctgctgcacc cggaccgcac cgtgatcaac
      241 ctcgccggcg acggcgactt cctgatgacg ggccaggaac tcgcgaccgc gaccggctac
      301 ggtgccggca ccgggccggg ccggctgatc gtgatcgtcg tcgacaacgg cagctacggc
      361 acgatccgca tgcaccagga gcgcgtgtac ccgggccgcg tcagcggcag cgacctgttc
      421 aaccctgact tcgcggcgct ggcccgcgcg caccgctgga acgccgcggt cgtcgacgcc
      481 acggcgcagt tcgagccggc cttggccgct gcgctgagca gcgaccggcc gacgctgatc
      541 cacgtcaagc tcgatcccga catcagcacc tcgcgcacga ccttgacggc gatccggcgc
      601 gtcgcgcgcg aacgctgaaa cggcccaccc cgaacccatg acccgcagaa aggcgaagaa
      661 gcgatgagca ccgattcaag actcgtgacc ggcgacctgt gcgacgcaca caaggccgac
      721 agcgacggcg gcttccgcgt gctgccgccg gtgttccgcg actacggcca gcgggttcgc
      781 ttcgccggta cggtcagcac ggtccaatgc ttcgaggaca actcgttcgt caaggccgcg
      841 ctcgaggcgc cggggcaggg ccgggtgctg gtggtcgacg gcggcgcatc gctgcggcgc
      901 gcgctggtcg gcggcaacat cgcggccgcg gcggcgcgca acggctgggc cggcgtcgtg
      961 atcgacggct gcgtgcgcga cagcgccgaa ctcgcgcagt gcgacctcgg catccgtgcg
     1021 ctggcgctgg tgccgatgcc gaccgagcgc cgcaatgccg ggcagcgcga tgtgccgttg
     1081 cagatccgcg gcgtccgggt gcagccgggc gactggctgg tggccgacga agacggcatc
     1141 gtcgtgatgc cggcggcgcc ggtctgacgc cagcaggatc gcgcgcctgc gcgcctggtc
     1201 ggcgctcagc tcaggcgggc aggccggccg ccagcagact gtcgcgatgg tgcgcgagat
     1261 cgtcggcgcg cttgtagtgc agcatcggca ggtagtcggc gtcgacgcgc agtgcggggc
     1321 gcagcctcaa ggcctcgccg gcgtgcaggg ccgcggcctg cgcatccccg acgcgcgcca
     1381 ggcaggccgc caggaaggcg tgctgcacgt cgcccagctt ggtcatgtgt tgcaggcact
     1441 cgaccgcctg ggcgtagcgc cgggcgacga agtgggcgcg cgcgagatgg ctccagaacc
     1501 ggtccgggtg gaacgggttc aggcgcatcg ccttcaggat ccattcgacg ccttcgtcgg
     1561 cgcggcccag ccaggtcagg atctcgccct gctgcacgac gatcaggtcg tcgttggggt
     1621 tcaggctgag cgcgcgctgc tggtggtaga cggcattgtc gaaattgtcc tggatcagga
     1681 agacggccgc gaggatgcgg tgcacgtcgc tgtcgttgtc gtccaggccg agcgccagcg
     1741 tcagctcgcg atggatctcg acctgctgcg cggcgaggtc cgtgcaccag ccgtaggtcc
     1801 aggcctggcc gaggatgcag ccgcgccagg cgtgcgcatg cgcgtagttc gggtcgagct
     1861 cggtcgcgcg cgtgatcagg cgcagcgcct cggtgttgtc gagcagcgtg gagcggtgat
     1921 gcagcacctt ggcggtcacg acgcattcgt aggccgccat gctttcggtc ggcttgcgtg
     1981 cggcacgctc gcgcgtcgcg gcttcgacgc ggcccggcag cacggcgacg atcgcgctcg
     2041 tcatctcgtc ctggatcgcg aagatgtcct cgagctggcg gtcgtagcgc tcggcccaga
     2101 catggcgatc ggcctcggcg tcgatcagct gcaccgtgat gcggatgcgc tcgccggcgc
     2161 gccgcacgct gccttcgagc acgtactgca cgcccaggtc gcgcgcgacc ttcttcacgt
     2221 cgaccgcctg gcccttgaac ttgaacgagg tgttgcgcga gatcacgaac agctcgcgaa
     2281 agcgcgacag ctcggtcagg atgtcctcgg tcagaccgtc gacgaagaac tcctgctcgg
     2341 ccacgccgct catgttgacg aagggcagca cgatgatcga cgcgccggtc gggcgcggcg
     2401 atgccgcgcc ggctgcttgg gccgcggcag cggccccggc cgcagcgtcg acgatcttgt
     2461 agacgcgtac cggcgcggcg atgttgtgca gctcgtgcag gcccatgtcg gcgaagtggc
     2521 agtcgagctt gcggcagacc tcgtcgtgga ccttgccgga gacgcagatc ccgccggcgt
     2581 cggccagtgc ctccaggcgc gccgcgatgt tgacgccgtc gccgtagatg cggccgtctt
     2641 cggcgagcac gtcgccgaga ttgacaccga tgcggaagtt catccgccgg tgctcgggcg
     2701 cgtcggcatt gcagctgcgc agctggatct gcatcgcgca ggcggcttcg accgcgtcga
     2761 ccgcgctgcc gaattcggcg agcatgttgt cgccggggct gtcgacgacg cggccgtggt
     2821 gggcgacgat gatgtcgctg acgaggctgc ggcacagcgt cagcgcgcgc acggtggcca
     2881 actcgtcgtc gcccatcagc cggctgaagc cggcggcgtc ggcgctcagg atggcggcga
     2941 gcttgcgctg cacgaatgct ccttgcgagt ggtcgggacc ctgcggtgcg ccgcgccggg
     3001 acggatcgcg gcgtcgcgat cgattctatt caaagcccga agagttcgag cgcgcggtcc
     3061 atgccgcctt gcaggatagc gaccatcgcc tgcgcctgca ccgccggttt ggggtggtag
     3121 gcgatgctca gcccggccac gcccatcatc ggcaggtcgt tggcgccgtc gccgaccgcg
     3181 atcgcctgcc tcggctcgac gcccatcagc tcgcagacct cgagcagcac gcgcttcttc
     3241 tcggcgccgt cgacgatgtc gccccagggc cggtcgacga gccggccggt gagggcgccg
     3301 gcctcgatct cgagctcgtt ggcgcgcgcg aaatccagcc cgagccgcgc gcgcacgcgc
     3361 tcgctgaaga acgtgaagcc gcccgacacg agcagggtct tcaggccggc ggcgcgacac
     3421 gcctgcacga aaggctcgac gccggggttg agctgcaggt gatttgcgta gacctcgtgc
     3481 agcgccgact cgggcacgcc cgcgagcagc gcgacgcgcc ggcgcaggct gtccttgtag
     3541 tcggcaatct cgccgcgcat cgccgcctcg gtgatggccg ccacctcggc cttgcgcccg
     3601 gcaacggcgg cgatctcgtc gacgcactcg atgttgatca gcgtggagtc catgtcgaac
     3661 gcgaccagct tgtagtcggt cagacgcagc ggcggggcga agccgcggat gaacaggccg
     3721 ggggcggatt cgtgggcggt catggcgtca ccccacgctc accttgagcg tgatgaggtc
     3781 gcgcttttcg gcgtcggtga gttcgatggt ggattcgtgc catgagttca ggcggtcaat
     3841 gcttgcttgg gcgtgccgag cgcgcgcagc acgtcgcgga ccaactgcgc gcggtccttg
     3901 ggctcgggcg tgtcgcgctc gatgcgcagc ttgtcgttgc cggccagctt gatgtgccgg
     3961 ttcttctgca ccagctcgat gatgcgcagc gcatcgaccg gcgggttcgg ccggaaactg
     4021 atcactgtga gaagcggcgc cgcgtcgatc ttggtcacgc cgaagggctt ggccagcacg
     4081 cgcaggcggt gcacgtcgaa cagcgtctgc ccctgcggcg ggagcttgcc gaagcggtcg
     4141 gtgacttcct cgagcatcgc gtcgatctgc tccgaggtgt tcgccgacgc gagccgcttg
     4201 tacagcgaca gccgcgtgtg cacatcgccg cagtacgcgt cgggcagcag cgccggcgcg
     4261 tgcaggttga tctcggtcgt ggccgacagc gggctcagca agtcgggttc gacacccatc
     4321 ttcagcgaac gcaccgcctc ggccagcatt tcgttgtaga gctggaagcc gacctccatc
     4381 atgttgccgc tctggtggtc gccgagcacc tcgccggcgc cgcggatctc gaggtcgtgc
     4441 atcgccaggt agaagcccga gccgagctcc tccatgctct ggatcgcttc gagccgcatc
     4501 gaggctgctt tcgtcaggcc ctcgacatcc ggcaccatca ggtaggcata ggcctggtgg
     4561 tgggagcgcc cgacgcgccc gcgcagctgg tgcagctgcg ccaggccgaa cttgtcggcg
     4621 cgcgacatga cgatggtgtt ggcgctcggc acgtcgatgc cggtctcgat gatggtcgag
     4681 cacagcagga ggttgtgccg ctgcgcgacg aagtcgcgca tcacgcgttc gagttcgcgc
     4741 tcgggcatct ggccgtgcgc gatcgcgatg cgcgcctcgg gcagcagctc gaccagcttg
     4801 tcgtggcgcg cctggatcgt ctcgacctcg ttgtgcagga agtagacctg cccgccacgc
     4861 ttgagctcgc gcagcaccgc ctcgcggatc gtgccgctgg tctcggtgcg cacgaaggtc
     4921 ttgatcgcca ggcgccgctg cggcgccgtc gcgatcaccg agagatcgcg caggccttcg
     4981 agcgccatgc ccagcgtgcg cgggatcggc gtggcggtca gcgtcagcac gtcgacctcg
     5041 gcgcgcattg ccttgatcgc ctctttgtga cgcacgccga agcggtgttc ctcgtcgatc
     5101 acgagcagcc cgaggcgcct gaacctgacc ttgtcgccga tcagcgcgtg ggtgccgacg
     5161 acgatgtcga ccgtgccgtc ggccaggcct tccagcgtgg tcttcacttc cttgctggag
     5221 cgaaagcgcg agatctcggc gacgcgcacc ggccacttgc cgaagcggtc cgagatgttc
     5281 tggtagtgct gttccgcgag cagcgtcgtc ggcgcgagga tcgcgacctg cttgccgccg
     5341 gtcaccgcga tgaacgcggc gcgcagcgcg acctcggtct tgccgaagcc gacgtcgccg
     5401 cagatcagcc ggtccatcgg ctgcggcgag accatgtcct ggatcaccgc atggatcgcg
     5461 gcctgctggt cgggcgtctc ctcgaaaccg aagctcgcgg cgaaggcctc gtagtcgtgc
     5521 ggctggaagc ggaacgcgaa accctcgcgc gcggcgcggc gcgcgtacag gttgagcagt
     5581 tcggcggcgg tgtcgcgcac ctgctcggcg gccttgcgtt tggccttgtc ccactggccc
     5641 gagccgagcc ggtgcagcgg cgcctcgtcg gcgctgacgc cggtgtagcg cgcgatcagg
     5701 tgcagctgcg cgaccggcac gtacagcgtc gccttgtcgg cgtactcgag gtgcaggaac
     5761 tcgctcggtc ccccttcgtc gttcccgagg tcgatgttgc gcagccccac gtagcggc
//
LOCUS       DATMJB010000145         2122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_750863, whole genome shotgun sequence.
ACCESSION   DATMJB010000145 DATMJB010000000
VERSION     DATMJB010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750863"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="VNU48_03240"
     CDS             complement(<1..97)
                     /locus_tag="VNU48_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867166.1"
                     /note="member of the SPOUT superfamily of RNA
                     methyltransferases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HWI10321.1"
                     /translation="MFNIVLVQPEIPPNTGNVIRLAANTGCALHLI"
     gene            complement(118..825)
                     /locus_tag="VNU48_03245"
     CDS             complement(118..825)
                     /locus_tag="VNU48_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HWI10322.1"
                     /translation="MLFSRLLDTVPSLCAICHGWGAQRVCAACVQRYGAALPRCRRCA
                     LQVPDGSAVCGACLTDPPPYASTIAAVDYDHPWDGLIAQFKFHAALDLTPALAAQMLA
                     ALQRSEAPAPTLLLPVPLSDARLRERGYNQAWELARRLGGALRCATDARLLLRVRDTP
                     HQLAFPPEKRAGNVRAAFAVEPRRRAELQGRSVTLVDDVMTTGATIAEAARTLLQAGA
                     AEVGVWVIARTPRHDAA"
     gene            846..1766
                     /locus_tag="VNU48_03250"
     CDS             846..1766
                     /locus_tag="VNU48_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HWI10323.1"
                     /translation="MPAAPDTHARRLDPIAVRAALRRMARAPQPPWLHAEVARRMAEK
                     LELILLQPERVIDWWSTLGAGAALLEQAYPKAQQLRVEPDARWAERGRAQAQRPWWTA
                     SRWTGGELQVALADDEIAPGAGLVWANMMLHAVVDPVALIERWHGLLRVDGFVMFSCL
                     GPGTLRELRTLYVRLGWPAPTPGFVDMHDLGDMLVHAGFADPVMDQETLTLRWNSPQA
                     LLAELRTLGGNTAPDRVAGLRTPRWRARLERELEKLAAPDGTLGLSFEVAYGHAFKAA
                     PRVRAGQPTTVSLDDMRAMVHSSHGRGKAP"
     gene            1851..>2122
                     /locus_tag="VNU48_03255"
     CDS             1851..>2122
                     /locus_tag="VNU48_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HWI10324.1"
                     /translation="MSATLSSHPSTALAATARPLRFGAEAGAGDHALQWLLERNCSTT
                     PRQMMGFYASLCLVSLAIGVFFWMQGASLVMPFASLEILAVGVALLV"
BASE COUNT          277 a          750 c          783 g          312 t
ORIGIN      
        1 cgatcaggtg cagcgcgcag ccggtgttgg cggccaggcg gatcacgttg ccggtgttcg
       61 gcgggatctc gggctgcacg aggacgatgt tgaacatggc gcgcgattgt ccctgcgtca
      121 cgcggcgtcg tggcgcggcg tgcgtgcgat cacccacacg ccgacctcgg cggcgccggc
      181 ctgcagcagc gtgcgcgcgg cctcggcgat ggttgcgccg gtggtcatca cgtcgtcgac
      241 cagcgtgacg ctgcgcccct gcagttcggc gcggcggcgc ggctcgaccg cgaacgcggc
      301 gcgcacattg ccggcgcgct tctcgggcgg gaaggcgagc tggtgcggcg tgtcgcgcac
      361 gcgcagcagc agccgcgcat cggtcgcgca acgcagcgcg ccgccgagcc ggcgcgcgag
      421 ctcccaggcc tggttgtagc cgcgctcgcg cagccgcgcg tcgctgagcg ggaccggcag
      481 caacagcgtc ggcgccggcg cctcgctgcg ctgcagcgcg gccagcatct gcgcggccag
      541 cgccggcgtc aggtcgagcg cggcgtggaa cttgaactgc gcgatcaggc cgtcccacgg
      601 gtggtcgtag tcgaccgcgg cgatcgtgct cgcgtagggt ggtgggtcgg tcaggcacgc
      661 cccacagacg gcgctgccat cgggcacctg cagcgcgcag cggcgacagc gcggcagcgc
      721 tgcgccgtag cgctgcacgc atgctgcgca cacgcgctgc gcgccccagc catggcagat
      781 cgcgcacagg ctcggcacgg tgtccagaag gcgggagaac agcacggctc aatatactgg
      841 cccgcatgcc tgccgcgccc gacacccacg cccgtcgcct cgacccgatc gcggtgcgcg
      901 cggcactgcg gcgcatggcg cgcgcgccgc agccgccgtg gctgcatgcc gaagtggcac
      961 gccgcatggc cgagaaactc gaactgatcc tgctgcaacc cgagcgcgtg atcgactggt
     1021 ggagcactct cggcgccggc gcggcgctgc tcgagcaggc ctacccgaag gcgcaacagt
     1081 tgcgtgtcga gcccgacgcg cgctgggccg agcgcggccg cgcgcaggcg cagcgcccgt
     1141 ggtggacggc gtcgcgctgg accggcggcg agctgcaggt cgcgcttgcg gacgacgaga
     1201 tcgcgcccgg cgccgggctg gtctgggcca acatgatgct gcacgcggtc gtcgacccgg
     1261 tcgcgctgat cgagcgctgg cacggcctgt tgcgcgtcga cggcttcgtg atgttctcgt
     1321 gcctgggccc cggcaccttg cgcgagctgc gcacgctgta cgtgcgcctg ggctggccgg
     1381 cgccgacgcc aggcttcgtc gacatgcacg acctcggcga catgctggtc cacgccggtt
     1441 tcgccgatcc ggtgatggac caggagacgc tgaccctgcg ctggaacagc ccgcaggcct
     1501 tgctggccga gctgcgtacg ctgggcggca acaccgcgcc cgaccgcgtg gccgggttgc
     1561 gcacgccgcg ctggcgcgcg cggctggagc gcgagctcga gaaactggcc gcgcccgacg
     1621 gcacgctcgg actgagcttt gaggtcgcct acggtcatgc gttcaaggcc gcgccgcgcg
     1681 tgcgcgcggg tcagccgaca acggtgtcgc tcgacgacat gcgcgcgatg gtgcattcca
     1741 gccacggccg gggcaaggca ccgtaggctc tgcgctaaac tctcgtggtt gagctcggcc
     1801 tgttccgcgc tcggtactct aggcagtcag ctggaatagg cacgcgcggg atgtcagcca
     1861 ccctttcttc tcacccctcc accgccctgg cggcaacggc acgcccgttg cgattcggcg
     1921 ccgaggcggg cgccggcgat cacgcactgc aatggctgct cgagcgcaat tgctcgacga
     1981 caccgcgcca gatgatgggc ttctacgcct cgctgtgcct ggtgtcgctg gcgatcggcg
     2041 tgttcttctg gatgcagggt gccagtctcg tgatgccgtt cgcatcgctc gagatcctgg
     2101 cggtcggcgt cgcgctgctg gt
//
LOCUS       DATMJB010000146         3146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_811704, whole genome shotgun sequence.
ACCESSION   DATMJB010000146 DATMJB010000000
VERSION     DATMJB010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811704"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1853)
                     /gene="tkt"
                     /locus_tag="VNU48_03260"
     CDS             complement(<1..1853)
                     /gene="tkt"
                     /locus_tag="VNU48_03260"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWI10325.1"
                     /translation="MANSIRALAMDAVQQANSGHPGAPMGMADMAVALWGRHLRHNPG
                     NPHWANRDRFVLSNGHASMILYALLHLTGYDLPMSELRNFRQLHSKTPGHPEHGLTPG
                     VETTTGPLGQGLANAVGMALAEKLLGQEFNRDGHAIVDHHTYVFLGDGCMMEGISHEA
                     SALAAAWKLGKLIALYDDNGISIDGHVTPWFIDNTPERFRAYGWNVIADVDGHDADAV
                     DDAIKQAIAQGSGSAPGGGKPTLICCKTTIGQGSPNRAGTAKAHGEALGAEEVRLTRE
                     ALGWTSEPFVIPEGAYTLWDARRQGLADEAAWVDAYAAYAKAYPELAVEFARRIDNEL
                     PKNWSQCVVDAAVAAQAKAESVATRKASQLALEVFTKELPEMLGGSADLTGSTLTNTS
                     SATPFRLEDDGRWNGGRHISYGVREFGMAAIMNGMALHGGLMPYGATFLTFSDYSRNA
                     IRMAALMKQRVIHVFTHDSIGLGEDGPTHQSIEHAASLRLIPGLDVWRPCDTAETVIA
                     WACAIESTARPSALLLSRQSVPYAPKKSLDGISKGAYVLAEPSEVGLKKKAQAVIIAS
                     GSEVQLALHAQQDLAKSGIAVRVVSMPSTTVFDRQPEAYKLGVLPKGLPRIA"
     gene            1986..2378
                     /locus_tag="VNU48_03265"
     CDS             1986..2378
                     /locus_tag="VNU48_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HWI10326.1"
                     /translation="MHGLHLTADLHDCPVARAAMTEPDALRAVCLAAVHAAGLLPVGE
                     LFHRFAPAPGASADAAVGITGVVLLAESHLAVHTWPEIAAVTLDAYVCNFGADNSARA
                     RALLEALIAAFEPKHIERHVLHRGAVRA"
     gene            2375..3082
                     /locus_tag="VNU48_03270"
     CDS             2375..3082
                     /locus_tag="VNU48_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWI10327.1"
                     /translation="MKAIILAAGRGERMRPLTDHTPKPLLAVRGKPLIEWHVQALARA
                     GVREIVVNTAWLEDRIVAALGDGARFGVAIRYSMEGRDFGGALETAGGIATALPWLVD
                     GPDDPFWVVSGDIFAPGFDFATAAVQRFADSAAQAHLWLVPNPPFHARGDFGLAADGH
                     GLADGPGPDGRTWTYANLALLRASLVAGIAPGTRAALGPLLFDGMRRRRITAEVFHGA
                     WENVGTPAQLEALNRGL"
BASE COUNT          395 a         1112 c         1128 g          511 t
ORIGIN      
        1 gcgatgcgcg gcaatccctt gggcagcacg ccgagcttgt aggcctcggg ctggcgatcg
       61 aacacggtcg tcgacggcat cgagaccacg cgcaccgcga tgccgctctt cgcgaggtcc
      121 tgctgcgcgt gcagcgcgag ctgcacctcc gagccgctgg cgatgatcac cgcctgggcc
      181 ttcttcttca ggccgacctc gctcggctcg gccagcacgt aggcgccctt cgagatgccg
      241 tcgagcgact tcttcggtgc gtacggcacg ctctggcgcg acagcagcag cgcgctcggg
      301 cgcgcggtgc tctcgatcgc gcaggcccac gcgatcacgg tctcggcggt gtcgcagggg
      361 cgccagacgt ccagccccgg gatcagccgc aacgaggcgg cgtgctcgat gctctggtgt
      421 gtcggcccgt cctcgccgag gccgatgctg tcgtgtgtga agacgtggat cacgcgctgc
      481 ttcatcagcg ccgccatgcg gatcgcgttg cggctgtagt cgctgaaggt caggaaggtg
      541 gcgccgtacg gcatcagccc gccgtgcagc gccatgccgt tcatgatcgc ggccatgccg
      601 aactcgcgca cgccgtagct gatgtggcgc ccgccattcc agcggccgtc gtcttcgagg
      661 cggaagggcg tggcgctgct ggtgttggtc agggtcgagc cggtcaggtc ggcgctgccg
      721 ccgagcatct cgggcagttc cttcgtgaag acttcgagcg caagctggct cgccttgcgg
      781 gtggcaacgc tttcggcctt ggcctgcgcc gcgaccgcgg catcgaccac gcactgcgac
      841 cagttcttcg gcagctcgtt gtcgatgcgg cgcgcgaact cgaccgcgag ttcggggtag
      901 gccttcgcgt acgcggcgta ggcgtcgacc caggcggcct cgtcggccag cccctggcgg
      961 cgcgcgtccc acagcgtgta ggccccttcg gggatcacga agggctcgct cgtccagccc
     1021 agcgcttcgc gcgtcagcct gacctcctcg gcgccgagcg cctcgccgtg cgccttcgcg
     1081 gtgccggcgc ggttcggcga gccctggccg atcgtggtct tgcagcagat cagcgtcggc
     1141 ttgccgccgc ccggggcgct gccgctgccc tgcgcgatgg cctgcttgat cgcgtcgtcg
     1201 acggcatcgg cgtcgtggcc gtcgacatcg gcgatcacgt tccagccgta ggcgcgaaag
     1261 cgctccggcg tgttgtcgat gaaccagggc gtgacgtggc cgtcgatcga gatgccgttg
     1321 tcgtcgtaca gcgcgatcag cttgccgagc ttccaggcgg cggccagcgc gctggcctcg
     1381 tggctgatgc cttccatcat gcagccgtcg ccgaggaaca cataggtgtg gtggtcgacg
     1441 atcgcgtgac cgtcgcgatt gaactcctgg cccagcagct tctcggccag cgccatgccg
     1501 accgcgttcg ccaggccctg gccgagcggg ccggtggtcg tctcgacgcc gggtgtcagg
     1561 ccgtgctcgg ggtggcccgg tgtcttgctg tgcagctggc ggaagttgcg cagctcgctc
     1621 atcggcaggt cgtagccggt caggtgcagc agcgcgtaca ggatcatcga cgcgtggccg
     1681 ttgctcagca cgaagcggtc gcgattcgcc cagtgcggat tgcccgggtt gtggcgcagg
     1741 tgccggcccc acagcgccac cgccatgtcg gccatgccca tgggcgcgcc ggggtggccg
     1801 gaattggcct gctgcaccgc gtccatcgcc agcgcgcgga tcgagttggc catcagggtc
     1861 gcgttcgtgg gagagagagt ggtggccatg cgggtccggt gggcggtggg caggaaagcc
     1921 cgccatttta ggggtggtcg cgcgctgcac cctgacccgg cgcgatgccc cggataattc
     1981 gcgcgatgca cggattgcac ctgaccgccg acctgcacga ctgtccggtc gcgcgcgccg
     2041 cgatgaccga gcccgacgcg ctgcgcgcgg tctgcctggc cgcggtgcac gcggccggcc
     2101 tgctgccggt cggcgaactc ttccaccgct tcgcgccggc gccgggcgcc agcgccgacg
     2161 ctgcggtggg catcaccggc gtcgtgctgc tcgccgagtc gcacctggcg gtgcacacct
     2221 ggcccgagat cgccgcggtg acgctcgacg cctacgtctg caacttcgga gccgacaact
     2281 cggcgcgcgc gcgtgcgctg ctcgaagcgc tgatcgccgc gttcgagccg aagcacatcg
     2341 agcggcacgt gcttcatcgc ggcgcggtcc gggcatgaag gccatcatcc tcgcggccgg
     2401 ccgcggcgag cgcatgcgcc cgctgaccga ccacacgccc aagccgctgc tggcggtgcg
     2461 cggcaagccg ctgatcgagt ggcatgtgca ggcgctggcg cgcgccggcg tgcgcgagat
     2521 cgtcgtcaac accgcgtggc tggaagaccg gatcgtcgcg gcgctcggcg acggcgcgcg
     2581 cttcggcgtc gcgatccgct attcgatgga aggccgcgac ttcggcggcg cgctcgagac
     2641 cgccggcggc atcgccaccg cgctgccctg gctggtcgat ggccccgacg acccgttctg
     2701 ggtcgtctcg ggcgacatct tcgcgcccgg cttcgacttc gccaccgccg cagtgcagcg
     2761 cttcgccgac agcgccgcgc aggcgcacct gtggctggtg ccgaacccgc cgttccacgc
     2821 gcgcggcgac ttcggcctgg ccgccgacgg ccacggcctg gccgacggcc cgggccccga
     2881 cggccgcacc tggacctacg cgaatctcgc gctgctgcgc gcgtcgctgg tcgccggcat
     2941 cgcgccgggc acgcgcgccg cgctcggccc gttgctgttc gatggcatgc gtcggcgccg
     3001 catcaccgcc gaggtcttcc acggcgcctg ggaaaacgtg gggacaccgg cccagttgga
     3061 agcgctgaat cgcggactat gatgttcatg aggacagccg ccccgatgag cacacgagag
     3121 tgcggcatgc cgatcgttgg ggccct
//
LOCUS       DATMJB010000147         5547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_395015, whole genome shotgun sequence.
ACCESSION   DATMJB010000147 DATMJB010000000
VERSION     DATMJB010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395015"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..499)
                     /locus_tag="VNU48_03275"
     CDS             complement(68..499)
                     /locus_tag="VNU48_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWI10328.1"
                     /translation="MVTKTKAPAAKRGAPRKRVAAAVEPVAETTVEAAKPNLIRAGLQ
                     ALDKARQDVAQRQSRVFEAILGFEPAQGLSGLVGREGGAAKVAAEAFGLRKFEAVFDQ
                     RVAHALERMGTPSADALNALIDEVAALKEEVRSLKAATRRR"
     gene            complement(604..1152)
                     /locus_tag="VNU48_03280"
     CDS             complement(604..1152)
                     /locus_tag="VNU48_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10329.1"
                     /translation="MSTQSLSAVASHIVGQYSQASKHVVDAYRYGAQRLVIGANTRYV
                     TFLKSGAVPLVSDGVKARIFKVQTRLTSLVENGINNGSDRAEKVIDFVAKGADGGIQR
                     VAAVAGRVEEALATQAITKVGNVAILPVAKISLTLATRTAEGTKRLSARIAGVESDVV
                     APVAAKAKRVVKKAVRGVKARA"
     gene            complement(1236..2828)
                     /locus_tag="VNU48_03285"
     CDS             complement(1236..2828)
                     /locus_tag="VNU48_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI10330.1"
                     /translation="MSRTHVLADRPHLWAFLAGCVAVTIGVLMHLPMFWMGRDNGFHL
                     ADMPMDAAMVWGMGLIVVGIAIAGFGLLPRQPSADAVAAQRLTVSAPEDAPLARAHWR
                     LMLVLTVALVIDVMKPASLGFVVPGMKVEYGVSKAMVAWLPFAALCGTVVGSVVWGLL
                     ADVYGRRASILLSAVMFVGTAICGAMPSFWWNVGMCFMMGAAAGGMLPVTYALLAETM
                     PSKHRGWVLVLVGGLGAVGGYLAASGCAAWLEPVLSWRVMWFLNLPTGLLLIFLNVFI
                     PESPKFLLAMGRHDEAHDTLRDFGCTVHEGGDAPVASTPAFERDEDFDIAQGMAARTW
                     ALSTTALSWGLINFGLLLWMPAHLVERGYSMLLSSELLAASALIAIPTVFVAALLYSR
                     WSSKGALLAAIVVTAAGLLGLIQLELATPGQISPVWAIALLIIGINGIIAMILPYAAE
                     SYPLSIRGRATGWIAACTKGGGLIAQLLSLLGLVPALFVAALLILVPVLLSLGLVWRC
                     CIETRGRDLRDLENVPILGQPY"
     gene            complement(2963..3667)
                     /locus_tag="VNU48_03290"
     CDS             complement(2963..3667)
                     /locus_tag="VNU48_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HWI10331.1"
                     /translation="MPVTTRRIPVRATEWRPSYRLIPSRYPTVGLYDRVADPADLDAV
                     FAIEALTNPRIRDDTGQLQLVPPAERVTGAGSTMVMAAFTHLNPEGSRFSDGGYGVYY
                     AAQTLATAIAEVGHHRAVFLARTHEPAIDVDLRTITASIDAELHDLRGLGKRGAGLFD
                     PNDYAAPQALGRQLRDAGSWGVQFPSVRDPGGLCVGVFRPRAIRQARSTTHIALHWDG
                     HRITHWYEKRAPHPLT"
     gene            complement(3667..4080)
                     /locus_tag="VNU48_03295"
     CDS             complement(3667..4080)
                     /locus_tag="VNU48_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HWI10332.1"
                     /translation="MNAVLQPSAAPVDLNSPAAAQAALRTFWRIADAWRLDPAEQATL
                     LGVGRTTLYQWKQGKVSAALDRHALERLSYLFGIYAALQILLPVAQQADAWVRQPNAA
                     PLFGGRSALERMLGGQVADLYVVRHYLDAQRGGKA"
     gene            complement(4204..5436)
                     /locus_tag="VNU48_03300"
     CDS             complement(4204..5436)
                     /locus_tag="VNU48_03300"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HWI10333.1"
                     /translation="MNSYIVGGAVRDELLKRPVVDRDWVVIGSSPQAMQEAGYVAVGA
                     DFPVFLHPVTKEEYALARTERKTAPGYRGFSFDAAPHVTLEEDLQRRDLTINAMARAE
                     DGSLIDPWGGQRDLADRVLRHVSPAFAEDPVRILRLARFAARFPEFSVAPETLALAAQ
                     MVGSGEVDALVPERVWQELARGLMEARPSRMFEVLRACGALGRLLPEVDRLWGVPQRA
                     DYHPEIDTGVHLMMVLDMAARLNGSLAVRFACLGHDLGKGTTPAEVLPRHLGHEQRSV
                     ELVRAVCDRLRVPVACRELAEAVAREHGNVHRSAEFGAAAILRLFERCDAVRRPDRFE
                     ELLLACECDARGRLGLSEAPYPQRERLTRALVIALGVDSAAVAAQAGARGLQGAAVGD
                     AIRAAREQAVAAGLAAAG"
BASE COUNT          862 a         1956 c         1946 g          783 t
ORIGIN      
        1 gaacagcgcg aggctggcct gcaggatgcg gtcacgggtt cgcgtggcga cgaccgtcat
       61 gcaggggtca acgtcgccgc gtggccgcct tcaggctgcg cacttcttcc ttcagcgccg
      121 cgacctcgtc gatcagcgcg ttcagcgcat cggccgacgg cgtacccatg cgctcgagcg
      181 cgtgcgcgac gcgctggtcg aacacggcct cgaacttgcg cagcccgaag gcttcggccg
      241 cgaccttggc ggccccgccc tcgcgcccga cgaggccgct caggccctgt gccggctcga
      301 acccgaggat cgcctcgaac acgcggctct gacgctgtgc cacgtcctgg cgcgctttgt
      361 cgagcgcctg caggccggcg cggatcaggt tcggtttcgc ggcctcgacc gtcgtctcgg
      421 cgaccggttc aacggcggcc gcgacacgct tgcgcggcgc accgcgcttg gcggcgggag
      481 ctttcgtctt cgtgaccatc aggtgcttcc ttcgcgaaca tgatgcccga caaacttcgg
      541 ctcgcgcatg cgcaaaggcc ctcggtttcc cgagggcctg cggttcatgc gggcgagcgc
      601 aggtcaggca cgggccttga cgccgcgcac ggccttcttg acgacgcgct tggccttggc
      661 ggccaccggc gcgaccacgt ccgactcgac gccggcgatg cgcgccgaca ggcgcttcgt
      721 gccttcggcg gtgcgggtgg ccagcgtcag cgagatcttc gcgaccggca ggatcgcgac
      781 gttgccgacc ttggtgatcg cctgggtggc cagggcttcc tcgacgcgac cggccaccgc
      841 agcgacgcgc tggatgccgc cgtccgcgcc cttggcgacg aagtcgatga ccttctcggc
      901 acggtcggaa ccgttgttga tgccgttctc gacgaggctg gtgagccgcg tctggacctt
      961 gaagatgcgc gcctttacgc cgtcgctgac cagcggcacg gcgccgctct tcaggaacgt
     1021 gacatagcgg gtgttcgcgc cgatgaccag gcgctgcgcg ccgtagcggt acgcgtcgac
     1081 cacgtgcttg ctggcttgcg agtactggcc gacgatgtgg ctggcgactg cgctgaggga
     1141 ctgggtgctc atgtgaaacg ctccgtggag ggttggggga tgaatgaatc atccgcgaca
     1201 agccctagtc ggcgaaagct agaagcgttc gaaacctagt acggttggcc taggatcggc
     1261 acgttctcca gatcgcgcag atcgcgcccc cgcgtctcga tgcagcagcg ccagaccagc
     1321 ccgagcgaca gcagcaccgg caccaggatc agcagcgccg cgacgaacag cgccggcacc
     1381 agcccgagca ggctcagcag ctgcgcgatc aggccgccac ccttggtgca ggccgcgatc
     1441 cagccagtcg cgcggccgcg gatggagagc gggtagctct cggccgcgta gggcaggatc
     1501 atcgcgatga tgccgttgat gccgatgatg agcagcgcga tcgcccagac cgggctgatc
     1561 tgcccgggtg tcgcgagctc gagctggatc agcccgagca ggccggcggc ggtgaccacg
     1621 atcgccgcga gcagcgcgcc cttgctgctc cagcggctgt acagcagcgc cgcgacgaac
     1681 acggtcggga tcgcgatcag cgccgaggcc gcgagcagct cgctcgacag cagcatgctg
     1741 tagcccctct cgaccaggtg cgcgggcatc cacagcagca gcccgaagtt gatcagcccc
     1801 cacgacagcg cggtggtgct cagcgcccag gtgcgcgcgg ccatgccctg cgcgatgtcg
     1861 aagtcttcgt cgcgctcgaa cgccggtgtc gacgcgacgg gggcgtcgcc gccctcgtgc
     1921 accgtgcagc cgaagtcgcg caaggtgtcg tgcgcctcgt cgtggcggcc catcgcgagc
     1981 aggaacttcg gcgactcggg aatgaacacg ttcaggaaga tcagcagcaa gccggtcggc
     2041 aggttcagga accacatgac gcgccagctc agcacgggct ccagccacgc cgcgcagccg
     2101 ctcgccgcga ggtagccgcc gaccgcgccg agcccgccga ccagcaccag cacccagccg
     2161 cggtgcttgg acggcatcgt ctcggccagc agcgcatagg tcaccggcag catgccgccg
     2221 gcggccgcgc ccatcatgaa gcacatgccg acgttccacc agaacgacgg catcgcgccg
     2281 cagatcgcgg tgccgacgaa catcaccgcc gacagcagga tcgatgcacg ccgcccgtag
     2341 acgtcggcca gcagccccca caccaccgag ccgacgaccg tgccgcacag cgcggcgaac
     2401 ggcagccagg cgaccatcgc cttcgacacg ccgtactcga ccttcatgcc gggcacgacg
     2461 aagcccaggc tggccggctt catgacgtcg atcacgagcg cgaccgtcag caccagcatc
     2521 aggcgccagt gggcgcgcgc cagcggtgcg tcttcgggcg ccgagaccgt caggcgctgc
     2581 gcggccaccg cgtccgccga cggctggcgc ggcaacagac cgaagcccgc gatcgcgatg
     2641 ccgacgacga tcaggcccat gccccagacc atcgccgcgt ccatcggcat gtcggcgaga
     2701 tggaagccat tgtcgcggcc catccagaac atcggcaggt gcatcagcac gccgatggtc
     2761 acggcgacgc agccggcgag gaaggcccac aggtgcgggc ggtcggcgag gacgtgggtg
     2821 cggctcattg acggcgacga tagcgcacgc cggtcggcgc agctgcgcgg gcgctgccga
     2881 ataccccggg tggcaggcac cggccgttgc gcacactgtg cttcgatcgg aaccggcgga
     2941 gacggggatg aatgagtgag ggtcaggtca acgggtgcgg ggcgcgcttc tcgtaccagt
     3001 gcgtgatgcg atggccgtcc caatgcagcg cgatgtgcgt ggtggagcgg gcctggcgaa
     3061 tcgcccgcgg ccggaacacg ccgacgcaca gaccgcccgg gtcgcgcacg ctcggaaact
     3121 gcacgcccca gctgccggcg tcgcgcagct gccggcccag cgcctgcggg gcggcgtagt
     3181 cgttcgggtc gaacaggccc gcgccgcgct tgcccaggcc gcgcaggtcg tgcagctcgg
     3241 cgtcgatcga cgcggtgatc gtgcgcaggt cgacgtcgat cgcgggctcg tgggtgcgcg
     3301 ccaggaacac ggcgcggtga tggccgacct cggcgatcgc cgtggccagc gtctgcgccg
     3361 cgtaatagac gccatagccg ccgtcggaga agcgcgagcc ctccgggttc aggtgcgtga
     3421 acgcggccat caccatcgtg ctgccggcgc cggtgacgcg ctcggccggc ggcaccagct
     3481 gcagctggcc ggtgtcgtcg cggatgcgcg ggttggtcag cgcctcgatc gcaaacaccg
     3541 cgtcgaggtc ggccgggtcg gcgacgcggt cgtacaggcc gacggtcggg tagcgcgacg
     3601 ggatcaggcg gtagctcggg cgccattcgg tggcgcgtac ggggatgcgg cgcgtcgtta
     3661 ccggcatcag gccttgccgc cgcgctgtgc gtcgaggtag tgacgcacga cgtagagatc
     3721 ggcgacctgc ccgccgagca tgcgctcgag cgcggatcgg cccccgaaca gcggcgcggc
     3781 gttcggctgg cgcacccagg cgtcggcctg ctgcgccacc ggcagcagga tctgcagcgc
     3841 cgcatagatg ccgaacaggt acgacaggcg ctccagcgca tggcggtcga gcgcggcact
     3901 caccttgccc tgcttccatt ggtacagggt cgtccggccg acgccgagca gcgtggcctg
     3961 ctcggccggg tcgagccgcc aggcgtcggc gatgcgccag aaggtgcgca gcgccgcttg
     4021 ggcggcggcc ggggagttca ggtcgaccgg cgcagcgctc ggttgaagga cggcgttcat
     4081 gcggcaagca tagttcagaa acggacgcaa ctcaataatt ggtccggtga tgaacacatg
     4141 tcttcagagt gtggatcggt gtcgatgtgc gcaggcgcgc cgcgcagacg ctgtcaccgg
     4201 gcgtcagccc gccgcggcca gcccagccgc caccgcttgc tcgcgcgccg cgcggatcgc
     4261 atcgccgacg gccgctcctt gcagcccgcg cgcgccggcc tgcgctgcga ccgccgcgct
     4321 gtcgacaccg agcgcgatca ccagcgcacg cgtcagccgc tcgcgctgcg ggtagggtgc
     4381 ctcgctcagg ccgagtcggc cgcgggcatc gcattcgcag gccagcagca gttcttcgaa
     4441 ccggtccggg cggcggaccg cgtcgcagcg ctcgaacagc cgcaggatcg ctgcggcgcc
     4501 gaactcggcg ctgcggtgca cgttgccgtg ctcgcgcgcg accgcttcgg ccagttcgcg
     4561 gcaggccacc ggcacgcgca gccgatcgca gaccgcgcgc acgagttcga cgctgcgctg
     4621 ctcgtggccg aggtggcggg gcaacacctc ggccggtgtc gtgcccttgc cgaggtcgtg
     4681 gcccaggcac gcgaagcgca cggccaggct gccgttcagg cgcgccgcca tgtcgagcac
     4741 catcatcagg tgcacgccgg tgtcgatctc ggggtggtag tccgcacgct gcggcacccc
     4801 ccacagccga tcgacctcgg gcaacaggcg ccccagcgca ccgcaggcgc gcagcacctc
     4861 gaacatccgt gacggtcgtg cctccatcaa gccgcgcgcc agctcctgcc agacgcgctc
     4921 gggcaccagc gcgtcgacct cgccgctgcc gaccatctgc gccgccagcg cgagggtttc
     4981 cggggccacg ctgaactcgg gaaaacgcgc cgcgaagcgc gccagccgca ggatccgcac
     5041 cggatcctcg gcgaacgccg gcgacacgtg ccgcaggacc ctgtcggcca ggtcgcgctg
     5101 gccgccccag gggtcgatga gcgagccgtc ttcggcgcgc gccatcgcgt tgatggtcag
     5161 gtcgcggcgc tgcaggtctt cctcgagcgt cacgtgcggg gccgcatcga agctgaaccc
     5221 gcggtagccc ggcgcggtct tgcgttcggt gcgggccagc gcatattctt ccttcgtgac
     5281 ggggtgcagg aagacgggga agtccgcgcc gacggcgacg tagcccgcct cttgcatcgc
     5341 ctgcgggctc gatccgatca cgacccagtc gcgatcgacc accggacgtt tcagaagctc
     5401 gtctcgaacc gcgccgccca caatgtagga attcatgaac ctcacggcag gaattgaacc
     5461 agttacatct tgatgcagat cgccccccca gtcatggggg accgatttgg agccggcttg
     5521 tttataaacc caacctgtgc ggtctgc
//
LOCUS       DATMJB010000148         2478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_276555, whole genome shotgun sequence.
ACCESSION   DATMJB010000148 DATMJB010000000
VERSION     DATMJB010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276555"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VNU48_03305"
     CDS             complement(<1..254)
                     /locus_tag="VNU48_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI10334.1"
                     /translation="MLRVDGVTVRFGGITALDKVSFAVAGGDIVGLIGPNGAGKTTLF
                     NCLSRLYACNEGTILFEGRPLVATPAHGIAALGIGRTFQNL"
     gene            complement(513..995)
                     /locus_tag="VNU48_03310"
     CDS             complement(513..995)
                     /locus_tag="VNU48_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10335.1"
                     /translation="MNRTERPGAYGVFKPVGHVIAAFPTDAAMQAAKTALMRRGFAAA
                     DVVAYSPEQMKRQADIDIEQAGVLAGIGQELNLVKSHRELAQQGHSFLVVHAPKETQA
                     RGVADIARRFHAARVQRYGHLMIEEMIEVGSGEKQVAESPARGLDAQTRSGREVGVGR
                     "
     gene            complement(1169..1954)
                     /locus_tag="VNU48_03315"
     CDS             complement(1169..1954)
                     /locus_tag="VNU48_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI10336.1"
                     /translation="MREATTTAAAAHIAAPSHLLQLLELRAAWELAAGIAALPWLRLA
                     PRGDGHAVLVLPGLVVSDASTKILRDFLTRRGYDVHGWGLGRNYGPRPGVEAAMLATI
                     DRLHAQSGRKLSLIGQSLGGAYARLLAAQRPDAVRSVITLGSPVGGHPRASNAWRVYE
                     FTSGKSSVDPSRWQQVTRAPQVPTTSIYSRSDGVVAWRTSIEPAARPQTENIEVVSSH
                     IGMAVHPAVLFAVADRLAQPEGRWQPFERRGWRAAVFPKPAAG"
     gene            complement(1987..>2478)
                     /locus_tag="VNU48_03320"
     CDS             complement(1987..>2478)
                     /locus_tag="VNU48_03320"
                     /inference="COORDINATES: protein motif:HMM:NF025338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWI10337.1"
                     /translation="KQLIVATLFRHFEERISACMEAAAEVDAIDDVWLSLHLIFETIN
                     AFRFVYRDVDYLLNEYPDLETRAQALTARNLLAARSLCSGLQRAGVIEAGAEEIEMLA
                     LQIVFAITCWFSFKRLTPRRSSAAAHAEPALAAYYALTFLSPYVVGEARDYLNYLRSK
                     YLR"
BASE COUNT          355 a          871 c          876 g          376 t
ORIGIN      
        1 aggttctgga aggtgcggcc gatgcccagc gcggcgatgc cgtgcgccgg tgtggccacc
       61 agcggccgac cctcgaacag gatcgtgcct tcgttgcacg cgtacagccg cgacaggcag
      121 ttgaacaagg tcgtcttgcc cgcgccgttc gggccgatca gcccgacgat gtcgccgccc
      181 gcgaccgcga acgacacctt gtcgagcgcg gtgatgccac cgaagcgcac ggtgacgccg
      241 tcgacacgca gcaactcggc gcgagagtcg gacgatgcga tggtcggctc cttgctcgga
      301 tcggacagcg gtcgctgcgg gcccgaagat accgaggcgg cggcgatttc aaacgaggct
      361 ttacccttgc actcgcgtct gcaggacaca gcgtccggcg cgcctgcaga gcatcgccga
      421 actgccgcgc agcacgatcg gcaaggtgct caagcgcgaa ctgcgcgagc gccacgttgc
      481 gagcggcctc gcgccgcgtt gacgcggagc ttctaacggc cgacgccgac ctcgcgcccg
      541 gaacgcgtct gcgcgtccag cccgcgcgcc ggcgactcgg cgacctgctt ctcgccggag
      601 ccgacctcga tcatctcctc gatcatcagg tgaccgtagc gttgcacgcg cgcggcgtga
      661 aagcgccgcg cgatgtcggc aacgccgcgg gcctgcgtct ccttcggcgc gtgcacgacc
      721 agaaagctgt ggccctgctg tgcgagttcg cggtgcgact tcaccaggtt cagctcctgg
      781 ccgatgccgg ccagcacgcc ggcctgctcg atgtcgatgt cggcctggcg cttcatctgc
      841 tcgggcgagt aggccaccac gtcggcggcc gcaaagccgc gccgcatcag cgctgtcttc
      901 gcggcctgca tggcggcgtc ggtcgggaac gccgcgatga cgtggccgac gggcttgaag
      961 acgccatagg cgccgggtcg ttcggttcgg ttcatctgca actcctttgc gcacggtggc
     1021 gagcccgcag gcccgatgca accaatcgtg tcaccgcggc gcggcggcgt cattcggccg
     1081 atcgagcatt cgcgcgtcgg cgctcgccta cagaaggcct gggcggttcc gtcccggcct
     1141 atgagggcac cggcaccgta ctcgccgatc agcccgcggc gggcttcggg aacaccgcgg
     1201 cgcgccagcc gcggcgctcg aacggctgcc accggccttc gggctgcgcg aggcggtccg
     1261 ccaccgcgaa cagcacggcc gggtgcaccg ccatgccgat gtggctgctg acgacctcga
     1321 tgttctcggt ctgcggtcgc gccgccggct cgatgctggt ccgccacgcg accacgccgt
     1381 cgctgcggct gtagatcgac gtggtcggca cctgcggcgc gcgcgtcacc tgctgccagc
     1441 gcgacgggtc gaccgagctc ttgccgctgg tgaactcgta gacgcgccag gcattgctgg
     1501 cgcgcggatg accgcccacc gggctgccca gtgtgatcac gctgcgcacc gcgtcgggcc
     1561 gctgcgccgc gagcagccgc gcgtaggcgc cgcccaggct ctggccgatc aggctgagct
     1621 tgcggccgct ttgcgcgtgc agccggtcga tcgtcgccag catcgcggcc tcgacaccgg
     1681 ggcgcgggcc gtagttgcgg cccaggcccc agccgtgcac gtcgtagccg cggcgcgtca
     1741 gaaagtcgcg caggatcttc gtcgacgcgt cgctcacgac cagccccggc agcaccagca
     1801 ccgcatggcc gtcgccgcgc ggcgcgagcc gcagccaggg cagcgcggcg atgccggcgg
     1861 cgagctccca ggccgcgcgc agctccagca gctgcaacag gtgcgatggc gcggcgatgt
     1921 gcgcggcggc ggcagtggtg gtggcttcgc gcatgcgatt gtgtctcctt gtcgtgatgt
     1981 ggggccctaa cgcaggtatt tcgagcgcag atagttcagg tagtcgcgcg cctcgccgac
     2041 gacgtagggc gacaggaagg tcagcgcgta gtacgccgcc aacgccggct cggcgtgcgc
     2101 cgccgcgctg ctgcgcctgg gcgtcaggcg cttgaagctg aaccagcagg tgatcgcgaa
     2161 cacgatctgc agcgcgagca tctcgatctc ttcggcgccg gcctcgatca cgccggcgcg
     2221 ctgcagcccg ctgcacaggc tgcgcgcggc cagcaggttg cgtgcggtca gcgcctgcgc
     2281 gcgcgtctcg aggtcggggt attcgttgag caggtagtcg acgtcgcggt agacgaagcg
     2341 gaacgcgttg atcgtctcga agatcaggtg cagcgacagc cacacgtcgt cgatcgcgtc
     2401 gacctcggcg gcggcttcca tgcaggccga gatgcgctcc tcgaagtggc ggaacagcgt
     2461 cgcgacgatc agctgctt
//
LOCUS       DATMJB010000149         4103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_647478, whole genome shotgun sequence.
ACCESSION   DATMJB010000149 DATMJB010000000
VERSION     DATMJB010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647478"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /gene="accD"
                     /locus_tag="VNU48_03325"
     CDS             <1..669
                     /gene="accD"
                     /locus_tag="VNU48_03325"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HWI10338.1"
                     /translation="LDAEGRYEIGHEVLPVDALKFKDSKKYPERLKDALDATGETDAL
                     VVVGGAVHSVPLVAACFEFDFMGGSMGSVVGERFVRGVESAIEQKVPFICFTATGGAR
                     MQEGLLSLMQMAKTNAALTQLAKARLPYISVLTDPTMGGVSASFAFLGDVVIAEPQAL
                     IGFAGPRVIENTVREKLPEGFQRAEFLLQTGAIDTIVDRRELRGRVARAIAMLQRQSA
                     DAVA"
     gene            656..1981
                     /gene="folC"
                     /locus_tag="VNU48_03330"
     CDS             656..1981
                     /gene="folC"
                     /locus_tag="VNU48_03330"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HWI10339.1"
                     /translation="MPSPETATSPLPTTLADWLARCERLHPKEIDMTLARTRRVKERL
                     GLQLSVPVIEVAGTNGKGSTCAMLEAIALQAGYRVGLYSKPHLVHFEERCRINGRMVD
                     GAALLPHFEAVEHARGDVSLTYFEFTLLAIARLLAETPLDLVILEVGMGGRLDAVNAF
                     DADCAIITSIAIDHAEYLGPDRETIAIEKAGVMRAGKPVIVSDPLPPQSLLDEAARVG
                     ADLWRFGRDFNYAGDQQQWRWAGRTRRFNALAYPALRGANQLLNASGALAAFETLRAQ
                     LPITAQAVRQGFATVELPGRFQIVAGQPTLVLDVAHNPHAVATLAQNLDQMGFFPRTH
                     AVFGAMADKDIAAILTRMAPLVDHWHFTDLAVPRAARADDLAAIHASLALAGPGPVTV
                     QRHAGPMQALDAALSAADPADRIVVFGSFHTVGGVLKDGTPRLAAKHAA"
     gene            2070..2873
                     /locus_tag="VNU48_03335"
     CDS             2070..2873
                     /locus_tag="VNU48_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWI10340.1"
                     /translation="MGLLSIFKRRADAPSPAGTAAETADAVLQARTRARQRLIGAAVL
                     VVIGIIGFPLVFETQPRPIPVDIPIELPRKDGAVPLVMPPARAPGTTSTASAVSAARP
                     AAPAPAPATGEIITESREDAGRAVNAASAPVAAVTPAPAKPDAAPAAKPATVAATEAP
                     PKPAESKPAPAVDGARARALLEGKPGAAAAESGRFVVQVGAFADANAAHETRLKVEKL
                     GLKTYTQVASTAAGNRIRVRVGPFGTRDEADKAMAKARGAGLSAVVLTL"
     gene            2882..3382
                     /locus_tag="VNU48_03340"
     CDS             2882..3382
                     /locus_tag="VNU48_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HWI10341.1"
                     /translation="MAVDPGWVDIGFAAFLVLSVLVGLMRGFVFELLSLAGWFAAYFV
                     GLWWTPSFQSHIHVGAPGSTLNYGATFACVFIVTLIVWSLGARLVRALIRATPLSPID
                     RLLGAGFGFARGLVVLLVVATVVGISPWGRAPAWQRSQGVAWLNGVLHELKPMFVNDV
                     SQPRSA"
     gene            3415..>4103
                     /locus_tag="VNU48_03345"
     CDS             3415..>4103
                     /locus_tag="VNU48_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWI10342.1"
                     /translation="MCGIVGVISKSPVNQLIYDALLLLQHRGQDAAGIVTMQGTKCFM
                     HKARGMVRDVFRTRNMRGLLGTVGLGQVRYPTAGNAYSEEEAQPFYVNAPFGIVLVHN
                     GNLTNAPALKQELVDIDRRHINTTSDTEVLINVVAHELALATRDQPLTPDLVFAAVRA
                     VHKRVKGSYAVVALIAGHGLLAFRDPFGIRPLCIGEGTGAEGREVMVASESVAIEGTG
                     HTVIRDVAPGEA"
BASE COUNT          593 a         1451 c         1462 g          597 t
ORIGIN      
        1 ctcgacgccg aagggcgcta cgagatcggg cacgaggtgc tgccggtcga tgcgctgaag
       61 ttcaaggaca gcaagaagta ccccgagcgg ctgaaggacg ccctcgacgc caccggcgaa
      121 accgatgcgc tggtggtcgt cggcggcgcg gtgcacagcg tgccgctggt ggccgcctgt
      181 ttcgagttcg acttcatggg cggctcgatg ggctcggtgg tgggcgagcg cttcgtgcgc
      241 ggcgtcgagt ccgcgatcga gcagaaggtg ccgttcatct gcttcaccgc caccggcggc
      301 gcgcgcatgc aggaaggctt gctgagcctg atgcagatgg ccaagacgaa cgccgcgctg
      361 acgcagctcg cgaaggcgcg cctgccgtac atcagcgtgc tgaccgatcc gacgatgggc
      421 ggcgtctcgg ccagcttcgc cttcctgggc gacgtggtga tcgccgagcc gcaggcgctg
      481 atcggcttcg ccggcccgcg cgtgatcgaa aacaccgtgc gcgagaaact gcccgagggc
      541 ttccagcgtg ccgagttcct gttgcagacc ggtgcgatcg acaccatcgt cgaccggcgc
      601 gagctgcgtg ggcgtgtcgc ccgcgccatc gcgatgctgc agaggcagag cgccgatgcc
      661 gtcgcctgaa acggcgacct ccccgctgcc cacgaccctg gccgactggc tcgcgcgctg
      721 cgagcgactg cacccgaaag agatcgacat gacgctggcg cgcacgcgcc gcgtcaagga
      781 gcggctcggg ctgcagctca gcgtgccggt gatcgaggtc gccggaacca acggcaaggg
      841 ctcgacctgc gcgatgctgg aggccatcgc gctgcaggcc ggctatcgcg tcggccttta
      901 ctccaagccg catctggtgc acttcgagga gcgctgccgc atcaacggcc ggatggtgga
      961 cggcgcggcg ctgctgccgc acttcgaggc ggtcgaacac gcgcgcggcg acgtgagcct
     1021 cacctacttc gagttcacgc tgctggcgat cgcgcggctg ctggccgaga cgccgctgga
     1081 cctggtcatt ctcgaggtcg gcatgggcgg gcggctggac gcggtgaacg cgttcgacgc
     1141 cgactgcgcg atcatcacca gcatagccat cgaccacgcc gagtacctcg gccccgaccg
     1201 cgagaccatc gcgatcgaga aggcgggcgt catgcgcgcc ggcaagccgg tgatcgtcag
     1261 cgacccgctg ccgccgcaaa gcctgctcga cgaggccgcg cgcgtcggtg ccgatctgtg
     1321 gcgcttcggg cgcgacttca actacgccgg cgaccagcag cagtggcgct gggccggccg
     1381 cacccggcgc ttcaacgcgc tggcctaccc ggcgctgcgc ggtgcgaacc agctgctcaa
     1441 cgccagcggc gctctggcgg cgttcgagac gctgcgtgcg caattgccga tcaccgcgca
     1501 ggcggtgcgc cagggcttcg cgacggtgga actgccgggc cgcttccaga tcgtcgccgg
     1561 ccagccgacc ctggtgctgg acgtcgcgca caacccgcac gcggtcgcca cgttggcgca
     1621 gaacctcgac cagatgggct tcttcccgcg cacgcacgcg gtgttcggtg cgatggccga
     1681 caaggacatc gcggcgatcc tgacgcgcat ggcgccgctg gtcgaccact ggcacttcac
     1741 cgacttggcc gtgccgcgcg cggcgcgtgc cgacgacctg gcggcgatac atgcgtcgct
     1801 ggcgctcgcc ggccccgggc cggtcacggt gcagcgtcac gccggcccga tgcaggcgct
     1861 ggatgcggcc ctgagtgccg ccgaccccgc tgatagaatt gtcgtcttcg gatcgttcca
     1921 caccgtcggt ggcgtgctga aggacggcac gcctcgactg gccgccaaac acgcggcctg
     1981 agggcctgcc gagcgcgcgt cgccgaggcc gcggcagatc gagaatccga caccacgccc
     2041 acctctcgtc gcaccggaat ccgcaccgca tgggtctgct gtccatcttc aagcgtcgcg
     2101 ccgacgcgcc gtccccggcg ggcacggcgg ccgagaccgc cgacgcggtg ctgcaggccc
     2161 gcacccgcgc gcggcaacgc ttgatcggcg ccgcggtgct ggtggtgatc ggcatcatcg
     2221 gcttcccgct ggtgttcgag acgcagccgc ggccgatccc ggtcgacatc ccgatcgagt
     2281 tgccgcgcaa ggacggcgcg gtccccctgg tgatgccacc ggcgcgtgca ccgggcacga
     2341 cctcgacggc ctcggcggtg tcggccgctc gaccggctgc tcctgcaccc gctccggcga
     2401 ccggcgagat catcaccgag tcacgcgagg acgcgggccg cgccgtgaac gcggcgtcgg
     2461 cacctgttgc ggccgtgact ccggcccccg ccaagcccga cgccgcgccg gccgcgaagc
     2521 ccgcaacggt ggcagccacc gaagcaccgc ccaaaccggc cgagtcgaag cccgcgcccg
     2581 ccgtcgacgg cgcgcgcgcc cgcgcgctgc tcgagggcaa gccgggcgcg gcggccgccg
     2641 agtcgggccg gttcgtcgtg caggtcggcg cgttcgccga cgcgaacgcc gcgcacgaga
     2701 cgcgcctgaa ggtcgagaag ctgggactga agacctacac gcaggtcgcg agcacggcgg
     2761 ccggcaaccg catccgcgtg cgcgtcggcc cgttcggcac gcgcgacgaa gccgacaagg
     2821 cgatggccaa ggcgcgcggc gccgggctga gcgcggtcgt gctgacgctg tgaggccggc
     2881 gatggcagtc gaccccggct gggtggacat cggcttcgcg gccttcctcg tgctctcggt
     2941 gctcgtcggc ctgatgcgcg gcttcgtgtt cgaattgctg tcgctggccg gctggttcgc
     3001 tgcgtacttc gtcggcctgt ggtggacgcc gtcgttccag tcgcacattc acgtcggtgc
     3061 gccgggctcg accttgaact acggcgcgac gttcgcatgt gtgttcatcg tcacgctgat
     3121 cgtctggagc ctcggtgcgc ggctggtgcg cgcgctgatc cgtgccacgc cgctgagccc
     3181 gatcgaccgg ctgctcggcg ccggcttcgg cttcgcgcgc ggcctggtcg tgctgctggt
     3241 ggtcgccacg gtcgtcggca tctcgccctg ggggcgagcg ccggcgtggc agcgctcgca
     3301 gggcgtggcc tggctgaacg gcgtgctgca cgagttgaag ccgatgttcg tcaacgacgt
     3361 ttcccaaccc cgctcggcgt gacgcgccgg ccgggctgaa tcgaggcaaa tcaaatgtgc
     3421 ggcatcgtgg gcgtgatctc caagagcccg gtcaatcagc tgatctacga cgcgctgctg
     3481 ctgttgcagc accgcggcca ggacgccgcc ggcatcgtca cgatgcaggg cacgaagtgc
     3541 ttcatgcaca aggcgcgcgg catggtgcgc gacgtgttcc gcacccgcaa catgcgcggc
     3601 ctgctcggca ccgtgggcct gggccaggtg cgctacccga ccgccggcaa cgcctacagc
     3661 gaggaagagg cgcagccgtt ctatgtgaac gcgccgttcg gcatcgtgct ggtgcacaac
     3721 ggcaacctga cgaacgcgcc ggcgctgaag caggaactgg tcgacatcga tcgccgccac
     3781 atcaacacca ccagcgacac cgaggtgctg atcaacgtcg tcgcccacga gctggcgctg
     3841 gcgacgcgcg accagccgct gacgccggac ctggtgttcg ccgcggtgcg cgccgtgcac
     3901 aagcgcgtca agggctcgta cgccgtggtc gcgctgatcg cggggcacgg gctgctcgcg
     3961 ttccgcgacc cgttcggcat ccgcccgctg tgcatcggcg aaggtaccgg cgcggaaggc
     4021 cgcgaagtga tggtcgcgag cgagtcggtc gcgatcgagg gcaccggcca caccgtgatc
     4081 cgcgacgtgg cgccgggcga ggc
//
LOCUS       DATMJB010000150         2002 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_39163, whole genome shotgun sequence.
ACCESSION   DATMJB010000150 DATMJB010000000
VERSION     DATMJB010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39163"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VNU48_03350"
     CDS             complement(<1..654)
                     /locus_tag="VNU48_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HWI10343.1"
                     /translation="MNAPVPLSQITTAASAAQHASRLREIPYNYTSFSDREIVIRLLG
                     ARAWEVLSRLRDERRTGRSARMLYEVLGDIWVVQRNPYLQDDLLDNPRRRKLLIEALH
                     HRLGEVEKRRTPTVDAERDAAVGELLQLASASVTTFAEQFEAVAELRRRTAKVLGRVT
                     AKDNIKFDGLSRVSHVTDATDWRVEYPFVVLMPDTEAEMAGLVKGCVELGLTIIPRGG
                     "
     gene            873..>2002
                     /gene="ugpB"
                     /locus_tag="VNU48_03355"
     CDS             873..>2002
                     /gene="ugpB"
                     /locus_tag="VNU48_03355"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HWI10344.1"
                     /translation="MKIQSLALAAGALALGLSGPAQAQVEVQWWHSMTGGLNDWVNDL
                     AKDFNASQKDYKVVPSYKGTYAESMTATMAAFRAGNAPHIVQVFEVGTATMMNAKGAV
                     KPVGEVMTQSGVAFDPKGYVPAVSAYYTAPNGQMMSFPFNSSTTIFYYNKDAFKAAGL
                     DPNKAPGTWPEVVAAAAKLKANGSKCPYTTSWMSWVHLESFSLWHNTLFATQNNGFGG
                     PGARLAFNSPLHLRHFENLSNMAKQGLFVYKGRDSVAVQSFVAGDCAMLTGSSGSYAD
                     VAKSGKFAYGLAPLPYYPDVAGAPQNTAIGGASLWVMAGKKPAEYKGVAEFFKYLSSP
                     EVQSASHKRTGYLPITMAAFQLTEKSGFYKDRPGTDVAVTQM"
BASE COUNT          354 a          667 c          665 g          316 t
ORIGIN      
        1 gccgccgcgc gggatgatcg tcaggccgag ctcgacgcag cccttcacca ggccggccat
       61 ctcggcctcg gtgtcgggca tcagcacgac gaacgggtac tcgacgcgcc agtcggtcgc
      121 gtcggtcacg tgcgagacgc gcgagaggcc gtcgaacttg atgttgtcct tggccgtgac
      181 gcggcccagc accttggccg tgcggcggcg cagttcggcc accgcctcga actgttcggc
      241 gaaagtcgtc accgacgcgc tggccagctg cagcagttcg ccgaccgccg cgtcgcgctc
      301 ggcgtcgacc gtcggcgtgc gccgcttctc gacctcgccg agacgatggt gcaaggcctc
      361 gatcaggagc ttgcggcggc gcgggttgtc gagcaaatcg tcctgcaaat acgggttgcg
      421 ctgcaccacc cagatgtcgc cgagcacctc gtagagcatg cgcgccgagc ggccggtgcg
      481 gcgctcgtcg cgcagccgcg agagcacctc ccaggcgcgt gcgcccagca ggcgaatcac
      541 gatctcgcgg tcggagaacg aggtgtagtt gtacgggatc tcgcgcaggc gcgacgcgtg
      601 ctgcgctgcc gatgcggcgg tcgtgatctg gctcagcgga acgggtgcgt tcataggggc
      661 cgggtgctct gcggcacccg cgttggcact tgagggtaca tgttaccgca ggcatgcggc
      721 actgcagcat gggccggcgg ccgcccctcg gtcccttgag gttgatcccg attgctgcga
      781 gttgccatca cagcgataac gcgactgtca caaacgaagc ccagactgcc gggctggcca
      841 cgagccgccc gcatttcttt cacggagttg tcatgaagat ccaatctctc gcgctggccg
      901 ccggtgcact ggcgctgggc ctgtcggggc cggcgcaggc ccaggtcgaa gtccagtggt
      961 ggcattcgat gactggcggc ctgaacgact gggtcaacga cctggccaag gatttcaacg
     1021 cgagccagaa agactacaag gtcgtgccca gctacaaggg cacctacgcc gaatcgatga
     1081 cggcgacgat ggccgccttt cgtgccggca atgcgccgca catcgtgcag gtgttcgagg
     1141 tcggcaccgc cacgatgatg aatgcgaagg gcgcagtcaa accggtcggc gaggtgatga
     1201 cgcaaagcgg cgtcgcgttc gacccgaagg gctatgtgcc cgcggtctcg gcctactaca
     1261 ccgcaccgaa cggccagatg atgtcgttcc cgttcaacag ctcgacgacg atcttctact
     1321 acaacaagga cgccttcaag gccgcgggcc tcgacccgaa caaggcgccg ggcacctggc
     1381 cggaagtggt cgccgccgcc gcgaagctca aggccaacgg cagcaagtgc ccctacacca
     1441 cgagctggat gagctgggtg cacctggaga gcttctcgct ctggcacaac acgctgttcg
     1501 cgacgcagaa caacggcttc ggcggcccgg gcgcgcgcct cgccttcaac tcgccgctgc
     1561 acctgcggca tttcgagaac ctgtcgaaca tggccaagca ggggctgttc gtctacaagg
     1621 ggcgagacag cgttgccgtg caatccttcg tcgccggcga ttgcgccatg ctgaccggct
     1681 cgtcgggctc ctatgccgac gtcgccaaga gcggcaaatt cgcctacggc ctggcgccgc
     1741 tgccctacta ccccgacgtg gccggcgcac cgcagaacac cgcgatcggc ggcgccagcc
     1801 tgtgggtgat ggcaggcaag aagccggccg agtacaaggg cgtggccgag ttcttcaagt
     1861 acctgtccag ccccgaggtg caatcggcca gccacaagcg caccggctac ctgccgatca
     1921 cgatggcggc gttccagctc accgagaagt cgggcttcta caaggacagg ccgggcaccg
     1981 acgtagccgt gacgcagatg at
//
LOCUS       DATMJB010000151         2172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_708481, whole genome shotgun sequence.
ACCESSION   DATMJB010000151 DATMJB010000000
VERSION     DATMJB010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708481"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1428
                     /locus_tag="VNU48_03360"
     CDS             199..1428
                     /locus_tag="VNU48_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWI10345.1"
                     /translation="MNAPDRLPASPEPHVMKTYGRLPVALSHGRGCWVWDTEGKKYLD
                     GLGGIAVNTLGHAHPKLVPALTEQIGKLIHTSNYYLVPLQEQLAAKLCELSGLTNVFF
                     CSTGLEANEAALKIARKYGHDKGIDRPEIVVYEAAFHGRSIATLSATGNAKVQAGFGP
                     LVEGFVRVPLNDVAAIEKVAATNKNVVAVFLEVIQGEGGVKPTTIEYLKAVRRLCDAN
                     GWLLMLDEVQCGMGRTGKWFAHQWAGIQPDVMPLAKGLGSGVPVGAIVCGPKAANVFG
                     PGNHGTTFGGNPLAMRAGVETIRIMEEDGLLANAATVGAALKAGLERELAGVEGVTDI
                     RGQGLMLGIELDRPCGELLGRAASAGLMISVTADKVVRLLPALILSADEAARIVAILC
                     PLIKDFLAAPAAAQPAS"
     gene            1446..>2172
                     /locus_tag="VNU48_03365"
     CDS             1446..>2172
                     /locus_tag="VNU48_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWI10346.1"
                     /translation="MKHYLQFKDFRAEEYAYLFERAATIKKRFKNYEKYTPLVDRTLA
                     MIFEKASTRTRVSFEAGMYQMGGSVVNLTTGDSQLGRAEPIEDSARVISRMVDIVMIR
                     TFEQEKIDAFAANSRVPVINGLTNEYHPCQILADIFTFIEHRGDIKGKVVAWVGDGNN
                     MANTWLQAADLLGFTVHVSTPGGYEVDEKLAFGGKPHRAGCYKVFADPMDACRGADLV
                     TTDVWTSMGYEAENEARLKAFADW"
BASE COUNT          384 a          730 c          738 g          320 t
ORIGIN      
        1 ggccgacgcc ccagacgcac cattgtgccg cgaatgtgtt gcagtgcagc acgatcggtg
       61 gcgacggggt tcacttgcat ccgtcgttca tcggcatttc acagcgcgga tttagaatcg
      121 ccttcgagcg ggatgcgcgc cgacgccagc aaggcgcgca cacccgctcg ttcatttctt
      181 ccctcggagt gaccgacgat gaatgccccg gacagactgc ccgcctcgcc cgaaccccat
      241 gtgatgaaga cctacgggcg tctgcccgtg gcgttgtcgc atggccgagg ctgctgggtg
      301 tgggacaccg agggcaagaa gtatctcgat ggcctgggcg gcatcgccgt caacacgctc
      361 gggcacgcgc acccgaagct ggtgcccgcg ctcaccgagc agatcggcaa gctgatccac
      421 acgtcgaact actacctcgt gccgctgcag gaacagctcg cggccaagct gtgcgagctg
      481 tccggcctga cgaacgtgtt cttctgcagc accggcctcg aggccaacga ggcggcgctg
      541 aagatcgcgc gcaagtacgg ccacgacaaa ggcatcgatc ggcccgagat cgtggtctac
      601 gaggccgcgt tccacggccg ctcgatcgcc acgctgtcgg ccaccggcaa cgccaaggtc
      661 caggccggct tcggcccgct ggtcgagggc ttcgtgcgcg tgccgctgaa cgatgtcgcg
      721 gcgatcgaga aggtcgcggc gacgaacaag aacgtggtcg cggtgttcct cgaggtgatc
      781 cagggcgaag gcggcgtgaa gccgacgacg atcgagtacc tgaaggcggt gcggcgcctg
      841 tgcgacgcga acggctggct gctgatgctc gacgaagtgc agtgcggcat gggccgcacc
      901 ggcaaatggt tcgcgcacca gtgggccggc atccagcccg acgtgatgcc gctggccaag
      961 ggcctgggct cgggcgtgcc ggtcggcgcg atcgtctgcg ggccgaaggc ggccaacgtg
     1021 ttcggcccgg gcaaccacgg caccaccttc ggcggcaacc cgctggcgat gcgcgccggc
     1081 gtcgagacca tccgcatcat ggaagaggac ggtctgctcg cgaacgctgc cacggtgggc
     1141 gcggcgttga aggccgggct cgagcgcgaa ctggccggcg tcgagggcgt gacggacatc
     1201 cgcggccagg gcctgatgct cggcatcgag ctcgaccgcc cgtgcggcga gctgctcggc
     1261 cgcgcagcca gcgccgggct gatgatcagc gtcaccgccg acaaggtcgt gcgcctgctg
     1321 cccgcgctga tcctcagcgc cgacgaggcg gcccggatcg tcgcgatcct gtgcccgctg
     1381 atcaaggact tcctcgccgc gcccgccgcc gcacaacccg cgtcatgaag cccggcagca
     1441 gcctgatgaa gcactacctg cagttcaagg acttccgcgc cgaggaatac gcctacctgt
     1501 tcgagcgcgc cgcgaccatc aagaagcgct tcaagaacta cgagaagtac acgccgctgg
     1561 tcgaccgcac cttggcgatg atcttcgaga aggccagcac gcgcacccgc gtgagcttcg
     1621 aggccggcat gtaccagatg ggcggctcgg tcgtgaacct gacgaccggc gacagccagc
     1681 tcggccgcgc cgagccgatc gaggacagcg cgcgcgtgat cagccgcatg gtcgacatcg
     1741 tgatgatccg caccttcgag caggagaaga tcgacgcgtt cgcggcgaac tcgcgcgtgc
     1801 cggtgatcaa cgggctgacg aacgagtacc acccgtgcca gatcctggcc gacatcttca
     1861 cgttcatcga gcaccgcggc gacatcaagg gcaaggtggt cgcgtgggtc ggcgacggca
     1921 acaacatggc caacacctgg ctgcaggcgg ccgatctgct gggcttcacc gtgcacgtga
     1981 gcacccccgg cggctacgag gtggacgaga agctcgcctt cggcggcaag ccccaccgcg
     2041 cgggctgcta caaggtcttt gccgatccca tggatgcctg ccgcggcgcc gacctggtga
     2101 ccaccgacgt gtggaccagc atgggctacg aggccgagaa cgaggcccgc ctgaaggcct
     2161 ttgccgactg gt
//
LOCUS       DATMJB010000152         4329 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_631318, whole genome shotgun sequence.
ACCESSION   DATMJB010000152 DATMJB010000000
VERSION     DATMJB010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631318"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..852)
                     /gene="ccmB"
                     /locus_tag="VNU48_03370"
     CDS             complement(184..852)
                     /gene="ccmB"
                     /locus_tag="VNU48_03370"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HWI10347.1"
                     /translation="MRALFAALFMRDLRLALRRPGEALLPIGFFVVAVALFPLGIGPE
                     PQTLRTIAPGLMWVCALLATMLSVSQLYAADHADGTLDQLMISGQSALVVAAARSAAH
                     WVLTGLPLVIAAPLFGAIFDLAPASIAMLAFTLLLGTPVLSLLGSVGAALTLGLRGAG
                     VLVILIVLPLTVPALIFGTGAVAAVEGGQSAAAHCSLLGALLIFSVLTAPLASAAALR
                     LAVE"
     gene            complement(852..1505)
                     /gene="ccmA"
                     /locus_tag="VNU48_03375"
     CDS             complement(852..1505)
                     /gene="ccmA"
                     /locus_tag="VNU48_03375"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HWI10348.1"
                     /translation="MHAASPASVTAPPLSALGLACERGSRRLFSDLAFELHAGEMLWV
                     RGRNGRGKTSLLRLVAGLGSAAQGQLCFDGRAARPARRRVVYIGHANALKEDASVLES
                     LAFLLRIHGGRNDAAALAAALAHWGLLAQQHALVRTLSQGQRRRVALARLAIERQPAL
                     WVLDEPYDALDANGIDCLNDLLGAHLQRGGSVLITGHQAALAAGLPRRELDLDDCAA"
     gene            2022..2921
                     /locus_tag="VNU48_03380"
     CDS             2022..2921
                     /locus_tag="VNU48_03380"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HWI10349.1"
                     /translation="MGTAIGVELWSEDRRAGVAAIDAVMDEMHRIDRVMSPHKPDSEL
                     SRINRDAAAAAVPLGAEMARLIERAQHFARLSGGAFDISYAAVGQLYDYRQRIRPSAA
                     ALAQARQCVGYRGLLLDTAAGTLRFARPGMRIDLGGFAKGHAVDNAAAILREHGIGHA
                     IVSAGGDSRVIGDRRGRPWTIGIRDPRRPGEMVALLPLEDVSISTSGDYERYFEDDGE
                     RFHHLIDPSTGRSPRGVHSVTVLAEDGLTTEALSKSVFVLGVDKGLRLIESQRGVDAV
                     VVDAGGALHYSAGLLAPTVPAPH"
     gene            2951..>4329
                     /locus_tag="VNU48_03385"
     CDS             2951..>4329
                     /locus_tag="VNU48_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10350.1"
                     /translation="MNKLWTAGGCAIAAALLAGCSGGSTSASSTVTVAGDVPIAYAMR
                     VNSIGANPTNGAPTAPGGDLFIREKSSASAVEHNITAQFTQGKGDASGPETSYDGKKI
                     VFSMHCPTSNTSTIGGQPACTGRWNIWEYDMTPSDKSAGGLTGGTFRRITASTDADDV
                     DATYLPAGRAFVFTSNRQTKSSINQALGHTYFALDEYERERVFNLHTMDADGGAITQI
                     SFNQSHDRSPVVRPNGDIMFSRWDHVGGRNHFKIFRAKPDGTDLFVLYGAHSEGNSFL
                     HPRDMDPKGAFAGSLTSDLMPLSRTHEGGGLVQIDVANYSEQNTPASPAASATGGQKQ
                     MTAQALNIGGGVSAYGRVTTPYPLWDGSDRVLLSYTPCEVSRRGVVVACATLSAAEMT
                     RLGDDNRLVADAQADELQNNVPPSYAVYMFDPAKQTFLIVAAPPTGFMYTHPVAIQAR
                     TEPNATDPTA"
BASE COUNT          735 a         1571 c         1462 g          561 t
ORIGIN      
        1 gggacgggcg cagcccgttc cgggggacat gagcggagtt gtccacccga ctctctcggc
       61 tcgcgccgac cgtcaaaggg cgcacgctcg cttgcgaacg accgcaacgg gccgcaagca
      121 gacatccgcc atgcgcgccg acgcccacga ctggccgcgt ggtttgcgac tggagtcttc
      181 atctcattcc accgcgagcc gcagggcggc cgcgctcgcg agcggtgccg tcagcacgct
      241 gaagatcagc agcgcgccga gcagcgaaca atgcgcggcc gccgactgcc cgccttcgac
      301 cgcagcgacc gcgccggtgc cgaagatcag cgccggcacc gtcaaaggca gcacgatcag
      361 gatcaccagc acgccggcgc cgcgcaggcc cagcgtcaat gccgcaccga ccgagccgag
      421 caggctcagg accggcgtgc ccagcagcag cgtgaaggcc agcatcgcga tcgatgcggg
      481 cgcgaggtcg aatatcgcac cgaacagcgg cgcggcgatc accagcggca aaccggtcag
      541 cacccagtgc gcggcgctgc gcgcggcggc gaccacgagc gcgctctggc cgctgatcat
      601 caactgatcg agcgtgccgt cggcatggtc cgcggcgtag agctggctca ccgagagcat
      661 cgtggccagc agcgcacaca cccacatcag gccgggcgcg atcgtgcgca aggtctgcgg
      721 ctcgggcccg atgcccagcg ggaacagcgc gaccgcgacg acgaagaagc cgatcggcag
      781 cagcgcctcg ccggggcggc gcagcgccag gcgcaggtcg cgcatgaaca gggcggcgaa
      841 gagcgcacgc atcaggccgc acagtcgtcc agatcgagtt cgcgccgcgg caggcccgcg
      901 gccagcgcgg cctggtggcc ggtgatcagc acgctgccgc cgcgttgcag atgggcgccg
      961 agcaggtcgt tcaggcagtc gatgccattc gcgtcgagcg cgtcgtaagg ttcgtcgagc
     1021 acccacagcg cgggctggcg ctcgatcgcg agccgcgcca gcgcgacgcg tcggcgctgc
     1081 ccttgcgaca gcgtgcgcac cagcgcgtgt tgttgcgcga gcagacccca gtgcgcgagg
     1141 gccgcggcca gcgcagcggc atcgttgcgg ccaccatgga tgcgcagcag gaacgcgagt
     1201 gactcgagga cgctcgcgtc ctccttcagc gcgttcgcgt gaccgatgta cacgacgcgc
     1261 cgccgcgccg gccgcgccgc tctgccgtcg aagcacagct gcccttgcgc cgcgctgccc
     1321 aggccggcga ccaggcgcag caggctggtc ttgccgcgac cgttgcggcc gcgcacccac
     1381 agcatctcgc cggcgtgcaa ctcgaacgcc aggtcgctga acaggcgtcg cgatccgcgc
     1441 tcgcacgcca ggccgagggc cgacagcggc ggggcggtga cagacgctgg cgaggctgcg
     1501 tgcaaggcgt ggttcgatga ctgcggaacc ggttggattg caagtcccgg gccgcgacag
     1561 cgcgcgctct cgcgcgctca tgcacgcgcg cacggtctca ttcagcagcc gcactcgggg
     1621 cctgcggcgg gcggcacaac gtgatcgcta cgtcctgata ccgacagccc gaatttgggt
     1681 cgggccagct cgttgcgctc actcacatct gaagcaactc aacgcacgcg cgctcaaaaa
     1741 tcgtcgttgc cgaccggatc gcgtggggtc ggattcgagc aagttaacaa atcccgacgc
     1801 tttttcacgc actcgatgcg atcgcgccac aggcacatga actgcgttgc gaaacggcga
     1861 ccggaagttg tgtcgagtcg acaccggcgc attcgtcacg agggcttcgc acgcacatgt
     1921 tcggcagttc cagacaagct tctcgcgcca tcatcgacct gccgccgcgt ctcttgcggg
     1981 ccgccgcgag tggcacctgg ctcaggcgcg aagaggccat catgggcacg gccatcggcg
     2041 tcgaactgtg gagcgaagac cgccgtgccg gcgtcgccgc gatcgacgcg gtgatggacg
     2101 agatgcaccg catcgatcgg gtgatgagcc cgcacaagcc cgactccgaa ctctcgcgca
     2161 tcaaccgcga cgcggccgcc gccgcagtgc cgctcggcgc cgagatggcg cgactgatcg
     2221 agcgcgccca gcacttcgcc aggctctcgg gcggtgcctt cgacatcagc tacgccgcgg
     2281 tcggccagct ctatgactac cgccaacgca ttcgcccgag cgcggcggca ctcgcgcagg
     2341 cacgccagtg cgtcggctac cgcgggctgc tgctcgacac ggccgcgggc acgctgcgct
     2401 tcgcgcgccc cggcatgcgc atcgacctcg gcgggttcgc caaaggccat gcggtcgaca
     2461 acgccgcggc gatcctgcgc gagcacggca tcggtcacgc gatcgtcagc gccggcggcg
     2521 acagccgcgt catcggcgac cggcgcggcc ggccctggac catcggcatc cgcgatccgc
     2581 gccggcccgg cgagatggtg gcgctgctgc cgctcgaaga cgtgtcgatc tcgacctcgg
     2641 gcgactacga gcgctacttc gaggacgacg gcgagcgctt ccaccacctg atcgatccgt
     2701 ccaccggccg ctcgccgcgc ggtgtgcaca gcgtcaccgt actcgccgag gacggcctga
     2761 cgaccgaggc gctgtccaag agcgtgttcg tgctgggcgt cgacaagggg ctgcgcctga
     2821 tcgagtcgca gcgcggggtc gacgccgtcg tggtcgacgc cggcggggcg ctgcactact
     2881 cggccggcct gctggcaccg acggtgccgg cgccgcactg aacatcacaa caacgatccg
     2941 gagggcgtcg atgaacaagc tttggaccgc gggcggctgc gcgatcgccg ccgctttgct
     3001 cgccggctgc agcggcggca gcacgagcgc ctcgagcacc gtgacggtcg ccggcgatgt
     3061 gccgatcgcg tatgcgatgc gcgtcaacag cataggcgcg aacccgacca acggcgcacc
     3121 gaccgcgccg ggcggtgacc tgttcatccg cgagaagtcg tcggccagtg cggtcgagca
     3181 caacatcact gcgcagttca cacaaggcaa gggcgacgcc tccgggcccg agacctcgta
     3241 cgacggcaag aagatcgtgt tctcgatgca ctgcccgacg tcgaacacct cgacgatcgg
     3301 cggccagccg gcctgcaccg gccgctggaa catctgggaa tacgacatga cgcccagcga
     3361 caagagcgcg ggcggcctga ccggcggcac gttccggcgg atcaccgcgt cgaccgatgc
     3421 cgacgatgtc gatgcgacct acctgccggc cggccgcgcc ttcgtgttca cctcgaaccg
     3481 ccagaccaag tcgagcatca accaggcgct cggccacacc tacttcgcgc tcgacgaata
     3541 cgagcgcgag cgcgtcttca acctgcacac gatggacgcc gacggtggcg cgatcacgca
     3601 gatctcgttc aaccagagcc acgaccgcag cccggtcgtg cggccgaacg gcgacatcat
     3661 gttctcgcgc tgggaccacg tcggcgggcg caaccacttc aagatcttcc gcgccaagcc
     3721 cgacggcacc gacctgttcg tgctgtacgg cgcgcacagc gaaggcaaca gcttcctgca
     3781 cccgcgcgac atggacccga agggcgcgtt cgccggctcg ctcacctccg acctgatgcc
     3841 gttgtcgcgc acccacgagg gcggcggcct ggtgcagatc gacgtggcga actactccga
     3901 gcagaacacg ccggcgagcc cggcagcatc ggccaccggc ggccagaagc agatgaccgc
     3961 gcaggcgctc aacatcggcg gcggcgtctc ggcctacggc cgcgtcacca cgccctaccc
     4021 gctgtgggac ggcagcgacc gcgtgctgct gtcgtacacg ccgtgcgagg tgagccgcag
     4081 gggcgtggtc gtggcctgcg cgaccctgag cgccgccgag atgacgcgcc tgggcgacga
     4141 caaccgcctc gtcgccgatg cccaggccga cgagctgcag aacaacgtgc cgccgtcgta
     4201 cgcggtctac atgttcgacc cggcgaagca gaccttcctg atcgtcgccg cgccgccgac
     4261 cggcttcatg tacacgcacc cggtcgcgat ccaggcgcgc accgagccga acgcgaccga
     4321 cccgaccgc
//
LOCUS       DATMJB010000153         3400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_631240, whole genome shotgun sequence.
ACCESSION   DATMJB010000153 DATMJB010000000
VERSION     DATMJB010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631240"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1794
                     /locus_tag="VNU48_03390"
     CDS             187..1794
                     /locus_tag="VNU48_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI10351.1"
                     /translation="MTAPLDPKPPGTGALTLETVELTKRFGSFTAMDSVSMTVAAGSV
                     HALLGENGAGKSTLVKCVAGSQRPEGGACLIDGREHDIATPVVARTLGIGMVYQHFTL
                     APGMNVAENLLLAGGRTPALIDWKKERAALQAFLQTTPFKLDLDATPAGLAAGEKQKL
                     ELLKQLYLKPRLLILDEPTSVLTPQEADEVLAYVRAVAQRGECTVLMITHKFREVMAY
                     ADEVTVLRRGKRVHGAQVARTTPEQLAQAMIGDSAASASAIVAEGSAAPRAATAADAV
                     VALRVEGLSAMGDRGTLAVQGLSLQVKRGEILGIAGVSGNGQRELVEALVGQRPRSAG
                     TVQVVGVPYGGRRAENRVLQLRALPEEPLRNACVGDLSVAQNMALRDFDEAPLKRGGW
                     LRFPAWRERARAWITEYGVKTRGENAPIASLSGGNVQRAVLARELAGQINVLIAANPV
                     FGLDFAAVAEIHARLLQVRAKGGAVLLISEDLDELLELSDRIAVMSEGRIVFETPAAG
                     ADRRVIGAHMGGAHHEDAHGHALPEAA"
     gene            1794..2471
                     /locus_tag="VNU48_03395"
     CDS             1794..2471
                     /locus_tag="VNU48_03395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWI10352.1"
                     /translation="MSAPIEIAATPFPYPFAPDHTALVVIDMQRDFVEPGGFGASLGN
                     DVSLLHAAIGPIAALLAAWRARGWPIVHTREAHLADLSDCPPAKRLRGSASLRIGDPG
                     AMGRLLIRGEPGTSIIAALAPQRGELVIDKPGKGMFWATGLHEMLKALEVTHLVFTGV
                     TTEVCVQTSMREANDRGYTCLLASDGTESYFPEFKAATLAMIAAQGAIVGWHAPSAAL
                     LGALPEA"
     gene            complement(2563..3045)
                     /locus_tag="VNU48_03400"
     CDS             complement(2563..3045)
                     /locus_tag="VNU48_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10353.1"
                     /translation="MFKRQHLVRSTLAVAMLSAAVLVQPAHAGLIGGLGGGGGLSGGF
                     GPRSIDFGGTGNAAGQISRSGAALPRADKAVQKAAGATEQAGTAAQPSAAPSGGATAG
                     QGAVGASSTPTRSGTTALDASAQAAHGRSATALDASAQAARGHGTTAADASAQGSVSR
                     "
     gene            complement(3167..>3400)
                     /locus_tag="VNU48_03405"
     CDS             complement(3167..>3400)
                     /locus_tag="VNU48_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWI10354.1"
                     /translation="AGGYVVARGTSFAAPLVAGLLAAALPAPDPALAPAALQRVAEQA
                     IDLGAPGRDPVFGIGLVGESARVAPDRVQARAR"
BASE COUNT          496 a         1216 c         1265 g          423 t
ORIGIN      
        1 gggacgggcg cagcccgttc cgggggacat gagcggagtt gtccacccga ctctctcggc
       61 tcgcgccgac cgtcaaaggg cgcacgctcg cttgcgaacg accgcaatgg gccgaaagca
      121 gagatccaaa gccgcagcag cgtttcacgc gtcgtgggtc gcgcaatgcg cggtggagca
      181 actgaaatga ccgcaccgct cgatccgaaa ccgcccggca ccggcgccct gacgctcgag
      241 accgtcgagc tgaccaagcg cttcggcagc ttcaccgcga tggattccgt cagcatgacg
      301 gtggccgccg gttcggtgca tgcgctgctg ggcgagaacg gcgccggcaa gagcacgctg
      361 gtgaagtgcg tggccggctc gcagcggccc gagggcggcg cgtgtctgat cgacggccgc
      421 gagcacgaca tcgccacacc ggtggtcgcg cgcacgctgg gcatcggcat ggtctaccag
      481 cacttcacgc tcgcgcccgg catgaacgtg gccgagaacc tgctgctcgc cggcggccgc
      541 acgccggcgc tgatcgactg gaagaaggaa cgcgccgcgc tgcaggcctt tctgcagacc
      601 acgccgttca agctcgacct cgacgccacg ccggccggcc tggcggccgg cgagaagcag
      661 aagctcgaac tgctcaagca gctctacctg aagccgcgcc tgctgatcct ggacgagccg
      721 acctcggtgc tcacgcccca ggaggccgac gaggtgctcg cctacgtgcg cgcggtcgcg
      781 cagcgcggcg agtgcacggt gctgatgatc acgcacaagt tccgcgaggt gatggcgtat
      841 gccgacgagg tgacggtgct gcgccgcggc aagcgcgtgc acggcgcgca ggtcgcgcgc
      901 accacgcccg agcagctcgc gcaggcgatg atcggcgact cggcggcgag cgcgtcggca
      961 atcgtcgccg aaggcagcgc cgcgccgcgc gccgcaaccg cggccgatgc ggtggtcgca
     1021 ttgcgggtcg agggcctgag cgcgatgggc gaccgcggca cattggccgt gcagggcctg
     1081 agcctgcagg tcaagcgcgg cgagatcctg ggcatcgcgg gcgtgtcggg caacggccag
     1141 cgcgaactcg tcgaggcgct cgtcggccag cgcccgcgca gcgccggcac ggtgcaggtg
     1201 gtcggcgtgc cgtacggggg gcggcgcgcc gagaaccgcg tgctgcagct gcgcgcgctg
     1261 cccgaggagc cgctgcgcaa tgcctgcgtc ggcgacctct cagtggcgca gaacatggcc
     1321 ctgcgcgact tcgacgaagc gccgctgaag cgcggcggct ggttgcgctt tccggcctgg
     1381 cgcgaacgcg cgcgcgcctg gatcaccgag tacggcgtga agacgcgcgg cgagaacgcg
     1441 ccgatcgcga gcctgtcggg cggcaacgtg cagcgcgcgg tgctggcgcg cgaactcgcc
     1501 ggccagatca acgtgctgat cgccgcgaac ccggtgttcg ggctggactt cgcggccgtc
     1561 gccgagatcc acgcgcgcct gctgcaggtg cgcgcgaagg gcggcgcggt gctgttgatc
     1621 agcgaagacc tcgacgagct gctcgagctc agcgaccgca tcgcggtgat gagcgaaggc
     1681 cgcatcgtgt tcgagacgcc cgccgcgggc gccgaccggc gcgtgatcgg cgcgcacatg
     1741 ggcggcgctc atcacgaaga cgcgcacggg cacgcgctgc cggaggccgc ctgatgagcg
     1801 caccgatcga gatcgcggcc acaccgttcc cgtacccgtt cgcgccagac cacacggcgc
     1861 tggtcgtgat cgacatgcag cgcgacttcg tcgagcccgg cggcttcggc gcgtcgctcg
     1921 gcaacgacgt gagcctgctg cacgccgcga tcgggccgat cgcagcgctg ctcgcggcgt
     1981 ggcgcgcgcg cggctggccg atcgtgcaca cgcgcgaggc gcacctggcc gacctgtcgg
     2041 actgcccacc cgcgaagcgc ctgcgcgggt cggcgagcct gcgcatcggc gacccgggcg
     2101 cgatggggcg gctgctgatc cgcggcgaac ccggcacctc catcatcgcg gcgctcgcgc
     2161 cgcagcgtgg cgaactcgtg atcgacaaac ccggcaaggg catgttctgg gcgaccggat
     2221 tgcacgagat gctgaaggcg ctggaggtga cgcacctggt gttcaccggc gtgaccacag
     2281 aggtctgcgt gcagacctcg atgcgcgagg cgaacgaccg aggctacacc tgcctgctcg
     2341 cctccgacgg caccgagagc tacttccccg agttcaaggc cgcgacgctg gcgatgatcg
     2401 cggcgcaggg cgcgatcgtc ggctggcacg cgccgagcgc ggcgctgctg ggggcgctgc
     2461 ccgaggcctg atatggcgcg acagccgcga cgggccctct ggttcagatc gcggcggcga
     2521 acagccggaa ccggccttcg ccggcccggc gggcccgggg catcagcgcg acaccgaccc
     2581 ctgcgccgaa gcatcggccg cggtcgtgcc gtggccgcgc gcggcttgcg cggaggcatc
     2641 gagcgcggtt gcgctgcggc cgtgcgcagc ctgcgccgag gcatcgagcg cggtcgtgcc
     2701 ggaacgggtg ggcgtcgacg acgcgccgac ggcgccctgg ccggcggtgg caccgccgct
     2761 cggcgccgcc gacggctgcg ccgcggtacc ggcctgctcg gtcgcgccag cggccttctg
     2821 caccgccttg tcggcacgcg gcagcgccgc gcccgagcgg ctgatctggc cggcggcgtt
     2881 gccggtgccg ccgaagtcga tgcttcgggg cccgaagccg ccgctcagac cgccgccgcc
     2941 gcccaggccg ccgatcaggc cggcgtgggc gggctgcacc agcacggccg ccgacagcat
     3001 cgcgacggcc agcgtggaac gaacgaggtg ttgacgcttg aacatgatga gttccttggg
     3061 ttcgaagggc cggccgatcc ggcccctacg acactcacaa cgggccgcgg cgacgctttc
     3121 ttccgcgcaa tcttgtaagg attcgtcagc gccggcgatc ggccgttcag cgggctcgcg
     3181 cctggacccg atccggcgcg acgcgcgcgc tctcgccgac gaggccgatg ccgaagaccg
     3241 ggtcgcgacc cggcgcgccg agatcgatcg cctgctcggc cacgcgctgc agcgccgccg
     3301 gcgccagcgc cgggtcgggc gccggcagcg ctgcggccag cagaccggcc accagcggcg
     3361 ccgcgaacga agtgccgcgc gcgacgacat agccgccggc
//
LOCUS       DATMJB010000154         2245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_38811, whole genome shotgun sequence.
ACCESSION   DATMJB010000154 DATMJB010000000
VERSION     DATMJB010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38811"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VNU48_03410"
     CDS             complement(<1..690)
                     /locus_tag="VNU48_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWI10355.1"
                     /translation="MSGCTLASQRRCAQRGLSLVEFLVGVAIALFIAAAAATLFAGNL
                     RENRQLLLEARLMQDLRTAADIATRDLRRAGYWADSTAGVWAAGASSVAPNPYVALAP
                     SGAASDAVSLRYSRDATENNLVDSNEQFGFRLKNGAIELQLGAGNWQALTDAGTLTIT
                     AFSVTPTLQDIDLGTFCATPCPAGSATCPPHQQVRSLALLISGRLVADASVTRSLRSQ
                     IRLRNDPVIGAC"
     gene            complement(690..1139)
                     /locus_tag="VNU48_03415"
     CDS             complement(690..1139)
                     /locus_tag="VNU48_03415"
                     /inference="COORDINATES: protein motif:HMM:NF028042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HWI10356.1"
                     /translation="MHRSNPARAQRGFTLIELMITVSIAGILSSVALPSFESQLQKAR
                     RSDVLLAAMQVQAAQERFRSNAASYGSLAEIGVPATTTAHHYTLQIAAYNADGYELLA
                     AATGAQARDAACRHMKLTATGMNLSYASGPDTALANPADANRKCWNL"
     gene            complement(1157..1702)
                     /locus_tag="VNU48_03420"
     CDS             complement(1157..1702)
                     /locus_tag="VNU48_03420"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HWI10357.1"
                     /translation="MKITLIRSGPQRGITLIEAAIVLAVTAIVAGSAAPSLQRVIDGR
                     RLEGAATQLATDIQLIRTEAVARNEALRLSFHAATGGSCYVIHTGAANECSCAASGPA
                     QCSGGAREIKTVTVAAADHVALQANVGSLLFDPLHGTSSPTGTLRLIGANDRAIHHVV
                     NIMGHVRSCSPQAAVPGYRAC"
     gene            complement(1834..>2245)
                     /gene="nrdR"
                     /locus_tag="VNU48_03425"
     CDS             complement(1834..>2245)
                     /gene="nrdR"
                     /locus_tag="VNU48_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWI10358.1"
                     /translation="QVVETRDSDEGDVVRRRRRCHSCDKRFTTYERAEIALPSVVKKD
                     GTRAEFDASKIRASMTLALRKRPVSVEQIDAALERIQDKLLNSGAKEVPSTRLGELVM
                     RELKRIDKVAYVRFASVYRSFEDVDEFRQLIRDI"
BASE COUNT          289 a          738 c          854 g          364 t
ORIGIN      
        1 gcaggcaccg atgaccgggt cgttgcgcag ccggatctgg ctgcgcaggc tgcgcgtcac
       61 gctcgcgtcg gcgacgagcc ggccgctgat cagcagcgcc aggctgcgca cctgttggtg
      121 cggtgggcag gtcgcgctgc cggccgggca cggcgtggcg cagaaggtgc cgaggtcgat
      181 gtcctgcagc gtcggggtga cgctgaaggc ggtgatggtc agcgtgccgg cgtcggtcag
      241 cgcctgccag ttgccggcgc cgagctgcag ctcgatcgca ccgttcttca ggcgaaagcc
      301 gaactgctcg ttgctgtcga cgaggttgtt ctcggtcgcg tcgcgggagt aacgcaagct
      361 caccgcgtcc gatgccgcgc cgctcggcgc gagcgcgaca taggggttcg gggcgacgct
      421 gctcgcgccg gcggcccaga cgccggccgt cgagtcggcc caatagccgg cgcggcgcag
      481 gtcgcgtgtg gcgatgtcgg ccgccgtgcg caggtcctgc atcagccgcg cttcgagcag
      541 cagctggcgg ttttcgcgca ggttgccggc gaacagcgtg gccgccgccg cggcgatgaa
      601 cagcgcgatg gcgaccccga ccaggaattc gaccagcgac aggccgcgct gcgcgcaacg
      661 gcgctgagag gccagcgtgc agccgctcat cacaggttcc agcacttgcg gttcgcgtcc
      721 gccgggttcg cgagcgccgt gtccgggccg gaggcgtagc tgaggttcat gccggtggcg
      781 gtcagcttca tgtggcggca ggcggcgtcg cgcgcctggg cgccggtcgc ggcggcgagc
      841 agctcgtagc cgtcggcgtt gtaggcggcg atctgcagcg tgtagtggtg ggcggtggtc
      901 gtggccggca cgccgatctc ggcgaggctg ccgtagctgg ccgcgttgct gcggaagcgc
      961 tcctgcgcgg cctgcacctg catcgccgcg agcagcacgt cgctgcggcg cgccttctgc
     1021 aattggcttt cgaagctggg cagcgcgacg ctggacagga tgccggcgat cgagaccgtg
     1081 atcatcagtt cgatcagcgt gaagccgcgc tgggcgcggg cggggttgga cctgtgcatg
     1141 tggttctcct tgggtttcag caggcgcggt agccgggcac cgcggcctgc ggcgagcagg
     1201 agcgcacgtg ccccatgatg ttgacgacgt ggtggatggc gcggtcgttg gcgccgatca
     1261 gccgcagcgt gccggtcggc gagctcgtgc cgtgcagcgg gtcgaacagc agcgagccga
     1321 cgttggcttg cagggcgacg tggtcggcgg cggccacggt cacggtcttg atctcgcgcg
     1381 cgccgccgct gcactgcgcc gggccgctgg ccgcgcagct gcactcgttc gcggcaccgg
     1441 tgtggatcac gtagcagctg ccgccggtgg ccgcgtgaaa gctcaggcgc agcgcttcgt
     1501 tgcgggccac ggcctcggta cggatcagct ggatgtcggt cgcgagctgg gtcgcggcgc
     1561 cctcgagccg acggccatcg atcacgcgct gcaggctcgg tgccgcgctg ccggcgacga
     1621 tcgccgtcac ggcgagcacg atggcggcct cgatcagcgt gatgccgcgc tgcgggcctg
     1681 aacggatgag ggtgattttc atctgcgctc cggggtgtga accgatgcgc agactttagg
     1741 aagcgcggcc gcagcgcgcg acccgcagag gtgggaggac gccggccccc tcgcgggctc
     1801 ccccgcgccg tcaccctcga tggggggatg cgttcagatg tcccggatca gctggcggaa
     1861 ctcgtccacg tcctcgaagc tgcggtacac cgaggcgaag cgcacatagg cgaccttgtc
     1921 gatgcgcttg agctcgcgca tcaccagctc gcccaggcgt gtggacggca cctccttagc
     1981 gccgctgttc agcagtttgt cctggatgcg ttccagcgcg gcgtcgatct gctcgacgct
     2041 gaccggccgc ttgcgcagcg cgagcgtcat cgacgcgcgg atcttggacg cgtcgaactc
     2101 ggcgcgcgtg ccgtccttct tgaccaccga cggcagcgcg atctcggcgc gctcgtaggt
     2161 cgtgaagcgc ttgtcgcagc tgtggcagcg ccggcgccgg cgcacgacgt cgccttcatc
     2221 ggaatcccgg gtctcgacga cctgg
//
LOCUS       DATMJB010000155         7152 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_157507, whole genome shotgun sequence.
ACCESSION   DATMJB010000155 DATMJB010000000
VERSION     DATMJB010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157507"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..311
                     /gene="acpP"
                     /locus_tag="VNU48_03430"
     CDS             69..311
                     /gene="acpP"
                     /locus_tag="VNU48_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWI10359.1"
                     /translation="MSDIEARVKKIIAEQLGVPEADVINDKAFVADLGADSLDTVELV
                     MALEDEFGIEIPDEEAEKITTVQLAIDYAVKHQKAA"
     gene            349..1596
                     /gene="fabF"
                     /locus_tag="VNU48_03435"
     CDS             349..1596
                     /gene="fabF"
                     /locus_tag="VNU48_03435"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWI10360.1"
                     /translation="MSSRRVVVTGLGLISPVGNTVEEGWANIIAGRSGIANITKFDAS
                     NFACKFAGEVKGFEVEDYFPAKDARHMDTFIHYGLAASIQAVKDSGLATGDELSEEQA
                     ERIGVLVGSGIGGLPLIEETHGELMAKGPRRITPFFVPASIINMISGHVSIRYGFKGP
                     NLAIVTACTTGLHSIGMAARLIEHGDADAMVAGGAESTVSPLGLGGFTAARALSTRND
                     DPATASRPWDKDRDGFVLGEGAGVLVLEEYEHAKARGAKIYAEIVGFGMGADAYHMTA
                     PDVDGPKRSMKAALRSAGLNADQVQYLSAHGTSTPLGDLNETNAIKLAFGAHAKDKLV
                     ISSTKSMTGHLLGGAGGIESVFTVLAVNRQIAPPTTNIFNQDPECDLDYCANTAREMK
                     IEHAVKNNFGFGGTNGTLVFKRV"
     gene            1633..2094
                     /locus_tag="VNU48_03440"
     CDS             1633..2094
                     /locus_tag="VNU48_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10361.1"
                     /translation="MRAAPAFQVSLRRFGVWRGAVLTVAILAMTTMAAWLSTQERPIG
                     IVAWLATGSSIAVAAALAARAARVPPVDLGWDGQTWSVRRNDEPAPGELHVAIDLGRW
                     MLLRFIPAAPAGAPAIWLPVQRSGLESQWHALRCAVYAPRPIPGADATGEA"
     gene            2105..2716
                     /gene="rpoE"
                     /locus_tag="VNU48_03445"
     CDS             2105..2716
                     /gene="rpoE"
                     /locus_tag="VNU48_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HWI10362.1"
                     /translation="MTKPDADAPLLERVKAGDVKAFEMLVVKYQRRIERLIGRMVRDV
                     DLVPDIAQETFIRAYRAIPQFRGDSAFYTWLYRIAVNTAKKALMELKRDPLVSESARG
                     ARDDDDDNASRVENELSNGETPDAVLASKQIASAVNFAIEALSDDLRQAITLREIEGL
                     SYEEIAELMNCPIGTVRSRIFRAREAIALRLRPLLDTRDGERW"
     gene            2735..3415
                     /locus_tag="VNU48_03450"
     CDS             2735..3415
                     /locus_tag="VNU48_03450"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HWI10363.1"
                     /translation="MTTNSETDAAARERLSALVDGELEDRAVAQACAHWRESPESRAT
                     WHSYQLIGDVLRSDDLAADAAREARFLVALRERLATEPVVLAPQPVAASRPGIHAEAA
                     RAASASTRRWSWMAPTAVAAGFVAVAGVLTLTRTSDVMAPARPAAASLAQATPALADP
                     VLARSTLAGPSLSSVPELQTLVANGDLIRDARLDRYLAAHKQFAGSTALGVPSGFLRN
                     ATAEAVGR"
     gene            3448..4476
                     /locus_tag="VNU48_03455"
     CDS             3448..4476
                     /locus_tag="VNU48_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HWI10364.1"
                     /translation="MLFRPLSALALAGAACIAAPASWAQQASAAGGPAQAREVRAWLL
                     RIHDAANHRNFQGTFVVSGGGAVSSARISHYCQGPNQYERSEALDGQARQVYRHNDDV
                     TTLWPATKVAMVEQRDLLAQFPALLQAGDDHIAEFYDVSPQGAERVAGREANVLVVRP
                     RDAFRYGYRLWADQASGLLLRADVIGERHEVLESSAFSDVAIDVKPQPESVLQPMRRL
                     DGYRVVRPELTPTRLEAEGWTMRQSAPGFRQVSCVKRPMEGTGAPDKTANRPQVLQSI
                     FSDGLTYVSVFIEPFDPRRHTRPMLASVGPTQTLMQQQGDWWVTVVGDVPPATLRVFA
                     KGLERSSK"
     gene            4620..6020
                     /locus_tag="VNU48_03460"
     CDS             4620..6020
                     /locus_tag="VNU48_03460"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWI10365.1"
                     /translation="MTGALAAPPVGAQGLPDFTELVEKVGPAVVGIRTTERNRAVRGG
                     NGEIDEDMLEFFRRFGIPIPNQRPNPRAPQQPQQPQPDPEPQTRGVGSGFILSADGYV
                     MTNAHVVDGAEEVVVTLTDSREFKAKIIGADKRSDVALVKIEAAGLPVVRIGDVNRLK
                     VGEWVIAIGSPFGLESTVTAGIVSAKARDTGDLVKLIQTDVAINPGNSGGPLINMRGE
                     VVGINSQIYSRSGGYMGISFAIPIDEATRVSDSLRANGRVIRGRIGVAIGPVTKEVAE
                     SIGLGKPIGALVSNVEAGGPADKAGVEAGDIITKVDGRAVEKFGDLPRMVGMTKPGAK
                     TALQIFRRGAYRDLSVTVVEFDAERGASGVPGREAAKPPAAAGMLGLTVAELSDAQRR
                     ELKIKGGVRVEGVEGAAARAGVREGDVLLSIDNTEVGSVKQFEAALAKLDKARAVTVL
                     VRRGDTVNFLIIRPQR"
     gene            complement(6243..6629)
                     /locus_tag="VNU48_03465"
     CDS             complement(6243..6629)
                     /locus_tag="VNU48_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10366.1"
                     /translation="MNQSIGERALAMVDVGNDRKVSDVIHACCDVLLVELVARTKRRS
                     RFSESSQSQSDVKARSAVLNIKKRHVEWMTRLPTKRMATACWDFIVFKRGPLQTAPLL
                     DFSPLQAANTKNNQQVIHNCLWMSWG"
     gene            6552..>7152
                     /locus_tag="VNU48_03470"
     CDS             6552..>7152
                     /locus_tag="VNU48_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014897649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWI10367.1"
                     /translation="MDHIRNFSIIAHIDHGKSTLADRLIQRCGGLSDREMEAQVLDSM
                     DIERERGITIKAQTAALEYTARDGRVYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTLANVYQAINNNHEIIPVLNKVDLPAAEPQRIRQQIEDVIGIDASDAVE
                     ISAKTGLGVPDVLEAIVHRLPAPKGDREAPLKALLIDSWY"
BASE COUNT         1219 a         2372 c         2445 g         1116 t
ORIGIN      
        1 tcttcgagaa gacaccccgg aggccgtaga atcgcgggct gtttctgtaa acactcccgg
       61 aggagttcat gagcgatatc gaagcacgtg tcaagaaaat cattgccgaa caactcggtg
      121 tccccgaggc cgacgtcatc aacgacaagg ccttcgtcgc cgatctcggc gccgattccc
      181 tcgacacggt ggaactcgtg atggcgctcg aagacgagtt cggcatcgag atccccgatg
      241 aagaggccga aaagatcacc accgtgcagt tggcgatcga ctacgccgtg aagcatcaga
      301 aggccgcctg agcgggcctt gccgatcaga actcagccgc agcagcccat gagcagtcgt
      361 cgtgtcgtcg tgacgggtct gggcctcatc agccccgtgg gcaacacggt ggaagagggc
      421 tgggccaaca tcatcgccgg gcgctccggc atcgccaaca tcaccaagtt cgacgcgtcg
      481 aacttcgcct gcaagttcgc gggcgaggtg aagggcttcg aggtcgagga ttacttcccg
      541 gccaaggatg cccgtcacat ggacaccttc atccactacg gtctggcggc atcgatccag
      601 gcggtgaagg attcgggcct ggccaccggc gacgaactca gcgaagagca ggccgagcgc
      661 atcggcgtgc tggtgggctc gggcatcggc ggcctgccgc tgatcgagga gacgcacggc
      721 gaactgatgg cgaagggccc gcggcgcatc acgccgttct tcgtgccggc ctcgatcatc
      781 aacatgatct cgggccacgt gtcgattcgc tacggcttca aggggccgaa cctggcgatc
      841 gtcacggcct gcacgaccgg cctgcacagc atcggcatgg cggcgcgcct gatcgagcac
      901 ggcgacgccg acgcgatggt cgccggcggc gccgagtcga ccgtctcgcc gctgggcctg
      961 ggcggcttca ccgcggcgcg cgcgctgtcg acccgcaatg acgatcccgc caccgcctcg
     1021 cgcccgtggg acaaggaccg cgacggcttc gtgctcggcg aaggcgccgg cgtgctggtg
     1081 ctcgaagagt acgaacacgc gaaggcgcgc ggcgcgaaga tctacgccga gatcgtcggc
     1141 ttcggcatgg gcgccgacgc gtaccacatg accgcgcccg acgtcgacgg cccgaagcgc
     1201 tcgatgaagg ccgcgctgcg cagcgccggg ctgaacgccg accaggtgca gtacctcagc
     1261 gcccacggca cgtccacgcc gctgggcgac ctgaacgaga ccaacgcgat caagctcgcg
     1321 ttcggcgcgc acgccaagga caagctcgtc atcagttcca ccaagtcgat gaccggccac
     1381 ctgctgggtg gcgccggggg catcgagtcg gtgttcaccg tgctcgcggt gaaccggcag
     1441 atcgcgccgc cgacgaccaa catcttcaac caggacccgg agtgcgacct ggactactgc
     1501 gccaacaccg cgcgcgagat gaagatcgag cacgccgtga agaacaactt cggcttcggc
     1561 ggcacgaacg gcacgctcgt cttcaagcgc gtttgacgcc gacgctgcca gcgctcatcg
     1621 attcccgcgc ccatgcgcgc agccccggca ttccaggtct cgctgcgtcg tttcggcgtg
     1681 tggcggggcg cggtgctgac cgtggccatc ctcgcgatga ccacgatggc ggcgtggctg
     1741 tcgacgcagg agcgtccgat cgggatcgtc gcctggctgg cgacgggatc gtcgatcgcc
     1801 gtggccgccg cgctggccgc gcgggccgcg cgcgtcccgc cggtcgacct gggctgggac
     1861 ggtcagactt ggtccgtccg ccgcaacgac gagccagcac ccggcgagtt gcacgtcgcg
     1921 atcgacctgg gccgctggat gctgctgcgc ttcattccgg ccgcgccggc cggtgcgccc
     1981 gcgatctggc tgccggtgca gcgctcgggg ctggaatcgc agtggcacgc gctgcgctgc
     2041 gcggtctatg cgccgcgccc gataccgggc gccgatgcga cgggcgaagc ctgacatgac
     2101 cggcatgacc aagcccgacg ccgacgcgcc gctgctcgag cgcgtcaagg ccggcgacgt
     2161 caaggccttc gagatgctgg tggtcaagta ccagcgccgc atcgagcggc tgatcggccg
     2221 gatggtgcgc gatgtcgacc tcgtgcccga catcgcccag gagaccttca tccgcgccta
     2281 ccgggcgatc ccgcagttcc gcggcgacag cgcgttctac acctggctct accgcatcgc
     2341 ggtgaacacc gcgaagaagg cgctgatgga actcaagcgc gacccgctgg tctcggaatc
     2401 ggcccgcggc gcgcgcgatg acgacgacga caatgcctcg cgggtcgaaa acgaactcag
     2461 caacggggaa acccctgacg ccgtgctggc gagcaagcaa attgcttccg cagtgaactt
     2521 tgcgatcgaa gcgctgtccg acgaccttcg ccaggcgatc acgctgcgcg agatcgaagg
     2581 tctcagctac gaagagatcg ccgagctgat gaattgcccg atcggcacgg tgcgttcgcg
     2641 gatcttccgc gcgcgtgagg cgatcgcgtt gcgactgcgt ccgctgctgg acacgcggga
     2701 cggggagcgg tggtgagctt tgacggagtg gcccatgacg acgaattctg aaaccgatgc
     2761 ggccgcgcgc gagcgcctgt cggcgctggt cgacggcgag ctcgaggacc gcgccgtcgc
     2821 gcaggcctgc gcgcactggc gcgaaagccc cgaatcgcgc gccacctggc attcctatca
     2881 attgatcggg gacgtgctgc gctcggacga cctggcggcc gatgcggcgc gcgaggcgcg
     2941 cttcctggtc gcgctgcgcg agcgcctggc gaccgagccg gtcgtgctgg cgccgcagcc
     3001 ggttgccgcg tcgcggcctg gcattcacgc cgaagcggcc cgggccgcga gtgcttccac
     3061 gcgccgctgg tcgtggatgg cgcccaccgc ggtggcggcc ggtttcgtcg cggtggcggg
     3121 cgtgctgacg ctgacgcgca ccagcgacgt gatggcgccg gctcggccgg cggccgccag
     3181 cctcgcgcag gcgacgccgg cgctggcaga cccggtgctg gcgcgatcga cgctggccgg
     3241 gccgtcgttg tcatcggtgc ccgagctgca gaccttggtc gccaatgggg acctgatccg
     3301 tgacgcgcgg ctcgatcgct acctcgcggc gcacaagcag ttcgccggca gcaccgcgct
     3361 cggcgtaccg tcgggctttc tgcgcaatgc gaccgccgaa gcggtcggtc gctgagcgtt
     3421 ttcgtgctgc gccactgccg ttcgcgaatg ctgtttcggc cgctgtccgc gctggcgctg
     3481 gcgggcgcgg cctgcatcgc ggcccctgcg agctgggctc agcaggcgag cgccgcgggc
     3541 ggcccggcgc aggcgcgtga agtgcgcgcc tggttgctgc gcatccatga cgcggcgaat
     3601 catcgcaact ttcagggcac cttcgtcgtc agcggcggcg gcgcggtgtc gagcgcgcgc
     3661 atttcgcact actgccaggg tccgaaccag tacgagcgca gcgaggcgct ggacgggcag
     3721 gcacgccagg tctatcggca caacgacgac gtgacgacac tttggccggc caccaaggtg
     3781 gcgatggtcg agcagcgcga cctgctggcg cagttcccgg cgctgctgca ggccggcgac
     3841 gaccacatcg ccgagttcta cgacgtgagc ccgcagggcg ccgagcgcgt cgcgggccgc
     3901 gaggccaacg tgctggtggt caggccgcgc gacgcgtttc gctacggcta tcgcctgtgg
     3961 gcggaccagg cctcggggct gctgctgcgc gccgacgtga tcggcgagcg ccacgaggtg
     4021 ctcgagtctt cggccttctc cgacgtggcc atcgacgtga agccccagcc cgagagcgtg
     4081 ctgcagccga tgagaaggct cgacggctac cgtgtcgtgc gacccgagct gacgccgacg
     4141 cgcctcgagg ccgaaggctg gacgatgcgc cagtcggcgc cgggctttcg ccaggtgagc
     4201 tgcgtgaagc gcccgatgga aggcacgggt gcacccgaca agaccgccaa ccggccgcag
     4261 gtgctgcaga gcatcttctc cgatggcctg acctatgtct cggtcttcat cgagcccttc
     4321 gatccgcggc gccacacccg gccgatgctc gcctcggtcg ggccgacgca aaccctgatg
     4381 caacaacagg gcgactggtg ggtgaccgtc gtcggcgacg tgccgcctgc gacactgcgt
     4441 gtcttcgcga aggggctgga gcgcagcagc aagtgaatgg cgccggccgc ccgttcgggc
     4501 cacggcaaac cagatccgca acgcctgggg aggcgatctt gatccaagca tctctgcatt
     4561 ccgttcaatc cgtgctgcgc cggcgcttgt gcctgggcgt cttggcgctc ggggtgggca
     4621 tgaccggcgc gctcgcggcg ccaccggtgg gcgcgcaagg cctgcccgac ttcaccgagt
     4681 tggtcgagaa ggtcggcccg gcggtggtcg gcatccgcac caccgagcgc aatcgggccg
     4741 tgcgtggcgg caacggcgag atcgacgagg acatgctcga gttcttccgc cgcttcggca
     4801 ttccgatacc gaaccagcgg ccgaacccgc gtgctccgca gcagccgcaa cagccgcagc
     4861 ccgaccccga gccgcagaca cgcggcgtgg gctccggctt catcctaagc gccgacggct
     4921 acgtgatgac gaacgcccac gtcgtcgacg gtgccgagga agtggtcgtc acgctgaccg
     4981 actcgcgcga gttcaaggcc aagatcatcg gcgccgacaa gcgctccgat gtcgcgctgg
     5041 tgaagatcga ggccgccggg ttgccggtgg tgcgcatcgg cgacgtgaac cggctcaagg
     5101 tcggcgaatg ggtgatcgcg atcggctcgc cgtttggcct ggaaagcacc gtcaccgccg
     5161 gcatcgtcag cgcgaaggcg cgcgacaccg gcgacctcgt caagctgatc cagaccgacg
     5221 tggcgatcaa cccgggcaat tcgggcggcc cgctgatcaa catgcgcggc gaagtcgtcg
     5281 gcatcaactc gcagatctac agccgctcgg gcggctacat gggcatctcg ttcgcgatcc
     5341 cgatcgacga ggcgacgcgc gtgtccgaca gcctgcgcgc gaacggccgc gtgatccgcg
     5401 ggcgcatcgg cgtggcaatc gggccggtca ccaaggaggt cgccgaatcg atcgggctgg
     5461 gcaagccgat cggcgcgctg gtgagcaatg tcgaggccgg cggcccggcc gacaaggccg
     5521 gcgtcgaggc cggcgacatc atcaccaagg tcgacggtcg cgccgtcgag aagttcggcg
     5581 atctgccgcg catggtcggc atgacgaagc cgggcgccaa gaccgcgctg cagatctttc
     5641 gacgtggcgc gtaccgcgac ctgagcgtca cggtcgtcga gttcgacgcg gagcgcggcg
     5701 ccagcggcgt gccgggtcgc gaggcggcga agccaccggc cgcggccggg atgctgggcc
     5761 tgacggtcgc cgagttgagc gacgcgcagc gtcgcgagtt gaagatcaag ggcggtgtgc
     5821 gcgtcgaagg cgtcgaaggg gcggcggccc gcgccggcgt tcgcgaaggc gatgtgctgc
     5881 tgtcgatcga caacaccgag gtgggcagcg tcaagcagtt cgaggccgcg ctcgccaagc
     5941 tcgacaaggc acgtgccgtc acggtgctgg tgcggcgtgg cgataccgtc aacttcctga
     6001 tcatccggcc gcagcgctga cgcgaacccg gtggaatcgc cgggtctttc catcaggtgg
     6061 gagcggtgac gaccgttaca tattcgccgt gacgatgggt agcggcgtgt tttgattcga
     6121 acgaaagtca gtgaacgctc acatcaataa gccaagctcg gattgttccc ggcgagggct
     6181 agaatcgcgg ctcgacgacc gggcacttta ctgattcgag caacgaaaac gacaggccga
     6241 aactatcccc atgacatcca caagcaattg tggataacct gttgattatt tttcgtgttg
     6301 gcggcctgca acggcgagaa atcgagcaac ggcgcggttt gcagcgggcc ccttttgaat
     6361 acaatgaagt cccaacaagc agttgccata cgttttgttg gaaggcgcgt catccattcg
     6421 acgtgccttt tttttatgtt cagcacggcg cttctcgcct tcacatcgct ttgcgattga
     6481 gagctttcgc tgaaacggct tcgccgtttt gtccgcgcga cgagctccac taaaagaaca
     6541 tcgcagcaag catggatcac atcagaaact tttcgatcat tgcccacatc gaccatggca
     6601 agagcacgct cgccgatcga ttgattcaac gttgtggtgg cctgtccgac cgcgagatgg
     6661 aggcgcaggt gctcgactcg atggacatcg agcgcgaacg cggcatcacg atcaaggcgc
     6721 agaccgccgc actcgagtac accgcgcgcg atggccgcgt ctacaacctg aacctgatcg
     6781 acacgccggg tcacgtcgac ttctcgtacg aagtcagccg ctcgctgtcg gcgtgcgaag
     6841 gggcgctgct cgtcgtcgat gcgagccagg gtgtcgaagc ccagacgctc gccaatgtgt
     6901 atcaggcgat caacaacaat catgagatca tcccggtcct caacaaggtg gacctgccgg
     6961 cggccgaacc ccaacgcatc cgccagcaga tcgaggatgt gatcggcatc gatgcctccg
     7021 atgcggtcga gatctctgcc aagaccggcc tcggggtgcc cgatgtgctc gaggccatcg
     7081 tccatcgcct gccggcaccc aaaggcgacc gagaggcccc gctcaaggcg ctcctcatcg
     7141 acagctggta tg
//
LOCUS       DATMJB010000156         2727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_532338, whole genome shotgun sequence.
ACCESSION   DATMJB010000156 DATMJB010000000
VERSION     DATMJB010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532338"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..887
                     /locus_tag="VNU48_03475"
     CDS             345..887
                     /locus_tag="VNU48_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10368.1"
                     /translation="MPQTYNVGARSLFVTATAWVFIILAALASVSALVQNAAVASLIP
                     GLQAATDRAPLPLLTGLLVGYLPWVVGMGLAMSLATLASAIGLLMRLDWARRTFIALL
                     VVAIFANLFGLWLQQEVLQSVVSSTLSSAPIPQQALGVFGGFVTAARVMAVLMTLVGC
                     ALLGWIIRRLMSAGVRQEFA"
     gene            complement(1063..2001)
                     /locus_tag="VNU48_03480"
     CDS             complement(1063..2001)
                     /locus_tag="VNU48_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HWI10369.1"
                     /translation="METRIATIRRFNRFYTGRIGVLHEGLLDSSFTLTESRLLWELAH
                     RERLTASELARTLDLDPGYLSRLLSGFKARGLIKRTRSKNDARHQHLSLTAAGKRAFA
                     PLDARSQAEVSTLLAPLQEVQQQQLLASMTSIERLLGAPVPRAAPFELRAHRAGDIGW
                     VIARHGALYAEEYGWDMGFEALVARIAADFIERFDPAREACWIAERDAAPIGSVFLVQ
                     ARDEITHAAIDGSAQLRLLLVEQSARGLGLGVRLVDECTRFARRVGYRKIVLWTNASL
                     LAARGIYRKAGYVLTHSEPHRSFGHDLIGETWELRL"
     gene            2049..>2727
                     /gene="imuA"
                     /locus_tag="VNU48_03485"
     CDS             2049..>2727
                     /gene="imuA"
                     /locus_tag="VNU48_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HWI10370.1"
                     /translation="MPEATMFQNLGACVDNCINVQSFVMDLLPASASPSPPVAASVAR
                     ALYRRADVPTPPEPAAAVSRRLPIRPESLHPTLWLGHQLARHADAAVATGFAALDAQL
                     PGAGWPRRALTELLLPHPGVGEIRLLAPSLVAAQRAGRLVMLFDPPAQLSAAALERLG
                     FAVEELLIINTRTRVIPGADSLWALEQALKSGHVGAVLAWLPPRLRAERLRRLQLAAH
                     AHDGPAFV"
BASE COUNT          375 a          951 c          964 g          437 t
ORIGIN      
        1 catcgccggg cggcgctgcc acgagcgcgt ctgcctcgac gacgcgctgc ggcccgacag
       61 ccgcgtgcat gtgatgggca gcaccaccgg caacctgtgg gcgagcgacg acggcgggga
      121 ccgctgggtg atggtgccgg tgaacctgcc gccgatccac gccgtacgct tcgattgacg
      181 ggcgcgcgtc ggccgcacca catcccgctg acaaaactgt ggcaaacatc gcacatcgag
      241 tcccaagaac atgggggcga cccccaggcg agggatgtgt tgtcacacgg agcggctgaa
      301 aatcgcgaca tgggcttgca agcccgttgt ttcgaggatc caccatgccg cagacctaca
      361 acgttggagc ccgttcactg ttcgttaccg ccacggcgtg ggtgttcatc atcctggcgg
      421 ccttggcgag cgtatcggcg ctggtccaga acgccgctgt cgcgtccctg atccccgggc
      481 tgcaggccgc caccgatcgc gcgccgctgc cgttgctgac cgggttgctc gtgggctact
      541 tgccgtgggt ggtcggcatg gggctcgcga tgtcgctggc gacgctggcg tcggcgatcg
      601 ggctgctgat gcgcctggac tgggcccgcc gcaccttcat cgcgctgctc gtcgtggcga
      661 tcttcgcgaa cctgttcggc ctgtggctgc agcaggaggt gctgcagtcg gtggtcagca
      721 gcacgctcag cagcgcgccg atcccgcagc aggcgctcgg cgtgttcggc ggcttcgtga
      781 ccgccgcccg cgtgatggcg gtgctgatga cgctggtggg ctgcgcgctg ctgggctgga
      841 tcatccggcg cctgatgtcg gccggcgtgc gccaggaatt cgcctgaact cacgcatcgt
      901 caacgcgcgt caccgtccag gtagaaccag cgctcgccct cgcgcacgaa gcggctgagt
      961 tcgtgcaggc cctcggcgcg ggcggatgca aggcaccgac gcggtagcgg ccgcagcagg
     1021 cctcgtaggg caaggcgtcg gcgggcgacc ggcaccggca gatcacaggc gcagttccca
     1081 ggtctcgccg atcaggtcgt ggccgaagct gcggtgcggc tcgctgtgcg tcaacacata
     1141 gcctgccttt cgatagatgc cgcgtgccgc gagcagactg gcattcgtcc acagcacgat
     1201 cttgcggtag ccgacgcggc gcgcgaaccg cgtgcattcg tcgaccagcc gcacgcccag
     1261 gcccaggccg cgcgccgact gctcgaccag cagcaggcgc agctgcgcgc tgccgtcgat
     1321 cgcggcgtgg gtgatctcgt cgcgcgcctg cacgaggaac acgctgccga tcggtgcagc
     1381 gtcgcgctcg gcgatccagc aggcctcgcg cgccgggtcg aagcgctcga tgaagtcggc
     1441 ggcgatgcgc gcgaccagcg cctcgaagcc catgtcccat ccgtactctt cggcatagag
     1501 cgcgccgtgg cgcgcgatca cccagccgat gtcgccggcg cggtgcgcgc gcagttcgaa
     1561 cggtgccgcg cgcggcaccg gcgcgccgag caggcgctcg atgctggtca tcgacgccag
     1621 cagttgctgc tgctgcacct cttgcagcgg ggcgagtaac gtgctcacct cggcctgcga
     1681 gcgcgcgtcg agcggcgcga acgcgcgctt gccggccgcg gtcaggctca ggtgctggtg
     1741 gcgcgcgtcg ttcttcgagc gtgtgcgctt gatcaacccg cgcgccttga agccgctcag
     1801 caggcggctc aggtagccag gatcgagatc gagcgtgcgc gccagttcgc tggcggtgag
     1861 gcgctcgcgg tgcgccagtt cccacagcag gcgcgactcg gtgagcgtga agctcgagtc
     1921 cagcaggcct tcgtgcagca cgccgatgcg gccggtgtag aagcgattga agcgacggat
     1981 ggtggcgatg cgggtttcca tcgggaggcg ccgagagcaa ggatgaatct cgattctggc
     2041 gatattagtt gccagaagca accatgtttc aaaacctcgg ggcttgcgtc gataactgta
     2101 taaacgtaca gtctttcgtc atggacctgc tgcccgcatc cgcctcgccg tcgccgcccg
     2161 ttgcggcgag cgtcgcgcgg gcgctgtacc ggcgcgcgga cgtgccgacc ccgccggagc
     2221 ctgccgcggc agtgtcgcgc cggctcccga tccgccccga gagcctgcac cccacgctct
     2281 ggctgggtca tcagctggcg cgccatgccg atgccgcggt cgccaccggc ttcgccgcgc
     2341 tcgatgccca attgcccggc gccggctggc cgcgccgcgc gctcaccgag ctgctgctgc
     2401 cgcatccggg cgtgggcgag atccggctgc tcgcccccag cctcgtcgcg gcccagcggg
     2461 cgggccggct ggtgatgttg ttcgacccgc cggcgcagct gtcggccgcg gcgctcgagc
     2521 ggctcggctt cgcggtcgag gaactgctga tcatcaacac ccgcacccgc gtgatccccg
     2581 gtgccgacag tctctgggcg ctcgagcagg cgctcaagag cggccatgtc ggtgcggtgc
     2641 tcgcctggct gccgccgcgc ctgcgcgccg agcggctgcg ccggctgcag ctcgccgccc
     2701 atgcccacga cgggccggcc ttcgtgc
//
LOCUS       DATMJB010000157         2264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_216261, whole genome shotgun sequence.
ACCESSION   DATMJB010000157 DATMJB010000000
VERSION     DATMJB010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216261"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..265)
                     /locus_tag="VNU48_03490"
     CDS             complement(74..265)
                     /locus_tag="VNU48_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10371.1"
                     /translation="MNSNRITTSTGIVIGGAYCEPPPRQSADADDIQRALLTRCRINR
                     FARAVGAALLATFLLRGKA"
     gene            complement(262..1722)
                     /locus_tag="VNU48_03495"
     CDS             complement(262..1722)
                     /locus_tag="VNU48_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HWI10372.1"
                     /translation="MGERRAADREDRCAGRRLAQVSEQGACNMTVKAFMSVRKNLVAP
                     LAHEATAMHIAETAAPAQAWPGAAEFLIGASLRDRGLHSATVPLTPGTAISTTATSTT
                     TTAAPKAVRWPSADSDLFILLAQAYIDCRRTKRNSPSALAFEAHAESNLFDLYRELLA
                     GTYRPGRSICFVVTHPRPREVWAAEFRDRIVHWLLYNAIAPRFHASFVADSCACIPGR
                     GTLYAARRLEHQVRSITRNWSAPASYLKCDLANFFVSIDKTVLLGQLRRRVTEPWWMA
                     LATTILMHDPREDVELRAGRRELALVPPHKSLFNAPDDHGLPIGNLSSQFFANVLLDE
                     LDQFVKHRIGAPHYVRYVDDFVLLHESATWLSGARVQIERKLGDLHLQLNPRKTILQP
                     IARGIDFVGFVVKPWRRTTRPRTLRAALQRLHEMPPDEVHQAANSYLGLLRQASHSHH
                     DRARFAGAALARGHCVNGAFTKVFRAHQSNPKARQE"
     gene            complement(1635..1997)
                     /locus_tag="VNU48_03500"
     CDS             complement(1635..1997)
                     /locus_tag="VNU48_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HWI10373.1"
                     /translation="MALHTDLPIYRTGTELLAMGYVIQRDMPRGFKRSLGEKIVMSCT
                     DMLELMALANATRGADRAANIERLLVLVRTATVTLRVAHDLRAIPTKQWASAVLLIEK
                     IGAQAGGWLKSANRAPAT"
     gene            complement(2028..>2264)
                     /locus_tag="VNU48_03505"
     CDS             complement(2028..>2264)
                     /locus_tag="VNU48_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10374.1"
                     /translation="DAAQAMAADAGLSAPTFQEARLIVAHRYGRLAGMSWFWLCEPHS
                     NAAYAWYCLLINGTVNYGNRSAEGGAVAVRRVNP"
BASE COUNT          370 a          708 c          802 g          384 t
ORIGIN      
        1 gagtggtatg cctcgttcgg cacgtcgtga tagacgcccg gcgcgatctg cgacaagtcg
       61 agcacggcgg cggtcatgcc ttcccccgaa gcaggaaggt ggccagcagc gcagcaccga
      121 cggcgcgcgc gaagcgattg atgcggcagc gggtgagcag cgcgcgctgg atgtcgtcgg
      181 cgtcggcgct ctggcgcggc ggcggttcgc agtaggcgcc gccgatcacg atcccagtag
      241 acgtggtgat gcggttgctg ttcattcctg cctcgctttc ggatttgatt ggtgcgcgcg
      301 gaacaccttg gtgaacgcgc cgttgacgca gtggccgcgc gccagcgccg cgccggcgaa
      361 gcgcgcgcga tcgtggtggc tgtgagacgc ttggcgcagc aggccgaggt agctgttcgc
      421 ggcctggtgg acctcgtcgg gaggcatctc gtgcaggcgc tgcagggcgg cgcgcaacgt
      481 gcgcggccgc gtggtccggc gccacggctt gacgacgaac ccgacgaagt cgatgccgcg
      541 cgcgatcggc tgcaggatcg tcttgcgcgg gttcagctgc aggtgaagat cgcccagctt
      601 gcgctcgatc tggacgcgcg ctccgctgag ccatgtcgcg ctctcgtgca gcagcacgaa
      661 gtcatcgacg taccgaacgt agtgcggcgc gccgatccgg tgcttgacga actggtcgag
      721 ctcgtcgagc agcacgttcg cgaagaactg cgacgacaga ttgccgatcg gcagaccgtg
      781 atcgtcgggt gcgttgaaca ggctcttgtg cggtggcacc agggcgagct cgcggcggcc
      841 cgcgcgcagt tcgacatcct cgcgcgggtc gtgcatcagg atcgtcgtcg ccagagccat
      901 ccaccacggc tccgtcacgc ggcgccggag ctggccgagc aacaccgtct tgtcgatgct
      961 gacgaaaaag ttcgccaggt cgcacttcag atagctcgcc ggcgccgacc agttccgggt
     1021 gatgctgcgc acctggtgct cgagccggcg cgcggcgtaa agcgtgccgc ggccgggaat
     1081 gcaggcgcat gagtcggcca cgaacgaagc gtggaaacgc ggtgcgatcg cgttgtacag
     1141 cagccagtgc acgatcctgt cgcggaactc ggccgcccac acctcgcgcg gtcgcgggtg
     1201 ggtaacgacg aagcagatgc tccggccggg ccggtaggtg ccggccaaca gttcgcggta
     1261 caggtcgaac agattgctct cggcgtgagc ctcgaatgcc agcgccgaag ggctgttgcg
     1321 cttcgtgcgc cggcaatcga tgtaggcctg cgccagcagg atgaacaggt ctgaatctgc
     1381 ggacggccac cgcaccgcct tcggcgctgc ggttgttgtt gttgacgttg ccgttgttga
     1441 gattgcagtt ccaggcgtaa gcggaactgt cgcgctatga aggccgcgat cccgaaggct
     1501 tgcgccgatc aggaactcgg ctgcaccggg ccaagcctgc gcaggcgcag cggtctccgc
     1561 aatgtgcatg gccgtggcct cgtgagccag gggcgcgacc agattcttgc gcacggacat
     1621 gaaggccttg accgtcatgt tgcaggcgcc ctgttcgctg acttgagcca gccgccggcc
     1681 tgcgcaccta tcttctcgat cagcagcacg gcgctcgccc attgcttcgt cgggatcgcg
     1741 cgcagatcgt gcgcgacacg aagcgtcacg gtcgcggttc gcaccagcac gagcaatcgc
     1801 tcgatgtttg cagcgcgatc ggcgccgcgc gtcgcgttag caagagccat cagctccagc
     1861 atgtcggtac agctcatcac gatcttctcg ccgagggatc gcttgaagcc gcgcggcatg
     1921 tcgcgctgga tgacgtagcc catcgcgagc agctcagtgc ccgtgcggta gatcggcagg
     1981 tcggtgtgca gggccatggc gtggctcagt tcaaaggatc aaagggttca aggattgact
     2041 ctgcggacgg ccaccgcacc gccttcggcg ctgcggttgc cgtagttgac ggtgccgttg
     2101 atgagaaggc agtaccaggc gtaagcggcg ttcgagtgcg gctcgcagag ccagaaccac
     2161 gacatgccgg cgaggcggcc gtaccgatgc gccacgatga ggcgcgcctc ttgaaacgtc
     2221 ggcgcggaga gcccggcatc ggccgccatg gcctgggcgg cgtc
//
LOCUS       DATMJB010000158         2441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_97485, whole genome shotgun sequence.
ACCESSION   DATMJB010000158 DATMJB010000000
VERSION     DATMJB010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97485"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..842
                     /gene="modB"
                     /locus_tag="VNU48_03510"
     CDS             171..842
                     /gene="modB"
                     /locus_tag="VNU48_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HWI10375.1"
                     /translation="MDSSLWTTLALTLKVAAWATAINLVLGVAVGWLLARRRFIGRKF
                     VDAVLTLPLVLPPTVMGYYLLVVIGKRGWLGDWLQTTFGINLIFTWQGAVIAATIVAF
                     PLVLKSARSAFETVDPQLESAARVLGLNEWAVFFRVTIPMAWPGILAGVLLVFARSLG
                     EFGATLMVAGSIPGRTQTLSIAVYEAVQAGQDDSANFLVAVTSITCIAVLLAAGRLAP
                     AGRTR"
     gene            852..1577
                     /locus_tag="VNU48_03515"
     CDS             852..1577
                     /locus_tag="VNU48_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI10376.1"
                     /translation="MGFDVDIRKTVHSARRSFDLRVSFKTRARRTVIFGPSGAGKSLT
                     LQAIAGLLRPDAGRIAFGDDVLFDAAAGIDVPARRRRFGYLFQDYALFPKLNVRQNIA
                     FGLSPGLRNPSSHANGEAVDRWLRSFELLDVAAQRPSELSGGQRQRTALARALVHSPR
                     ALLLDEPFSALDPELRERTRAELDELLRRIDIPVLMITHDPEDLEWFGDEALYLREGA
                     IAERPAPARPRDSMKPWPLAPTH"
     gene            1553..2365
                     /locus_tag="VNU48_03520"
     CDS             1553..2365
                     /locus_tag="VNU48_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HWI10377.1"
                     /translation="MAARAHPLTLGAELWLSLDGHNLAGARRVALLAEIARVGSITQA
                     AKTVGFSYKGAWNAIEDMSNLAGEPLLDRVVGGKGGGHTRLTQRGEKLVHNFELIRRE
                     HARFVDRLNRQANGLTEDYSWVGSIALKTSARNQFAGTVRAIRDGATNDEIELEVIGG
                     LRIVATVTRESRAELGLDVGAKAFALVKASSIMLMTDAGDVRLSARNQLAGTVTRVVP
                     GAVNTEVVLELPGGGTVAAIVTNASARSLGIGDGSPATAVFKASSVIVGVAA"
BASE COUNT          360 a          826 c          873 g          382 t
ORIGIN      
        1 caaggtcgtg gcgacggtcc cgaacgacac cccgatcacc tacccggtcg ccgccgtcag
       61 cggcagcccc aaccccgagg ccgcgcgcag gttcctcgac ttcctggtcg cccctgccgc
      121 gcaggccgtg ctggcacgcc acggcttcgg caagccgtag cggcggtcgg atggattcgt
      181 cgttgtggac cacgctggcg ctgacgctga aggtcgccgc gtgggcgacg gcgatcaacc
      241 tggtgctcgg cgtggcggtc ggctggctgc tcgcgcgccg ccgcttcatc ggtcgcaaat
      301 tcgtcgacgc ggtgctgacg cttccgctgg tcctgccgcc gaccgtgatg ggctactacc
      361 tgctggtggt gatcggcaag cgcggctggc tcggcgactg gcttcagacg acgttcggga
      421 tcaacctgat cttcacctgg caaggcgcgg taatcgcggc cacgatcgtg gcattcccgc
      481 tggtgctgaa gtcggcgcgc agcgcgttcg agacggtcga tccgcaactc gaaagcgccg
      541 cgcgcgtgct cggcctgaac gaatgggccg tcttctttcg cgtgacgatc ccgatggcct
      601 ggccgggcat cctggccggc gtgctgctgg tgttcgcccg ctcgctcggc gagttcggcg
      661 cgacgctgat ggtggcgggc agcatccccg gtcgcacgca gacgctctcg atcgcggtct
      721 acgaggcggt ccaggccggg caggacgaca gcgccaactt tctggtggcc gtcacgtcga
      781 tcacctgcat cgccgtgctg ctggccgccg ggcggttggc gcctgcaggg cgcacgcgct
      841 gaggcatgcg catgggtttc gacgtcgaca tccgcaagac cgtgcacagc gcgcggcgca
      901 gtttcgatct gcgggtcagc ttcaagacgc gcgcgcgccg caccgtcatc ttcggcccgt
      961 cgggcgccgg caagagcctg acgctgcagg ccatcgccgg gctgctgcgc cccgacgcgg
     1021 gccggatcgc cttcggcgac gacgtgctgt tcgacgccgc tgcaggcatc gacgtgcccg
     1081 cccgccggcg ccgattcggc tacctgttcc aggactacgc gctgtttccc aagttgaacg
     1141 tgcgccagaa catcgcgttc ggcctgtcgc cggggctgcg caacccgtcg tcgcatgcga
     1201 acggcgaagc ggtggatcgc tggctgcgca gcttcgaact gctcgatgtc gcggcgcagc
     1261 ggccgagcga gctgtcgggt ggccagcggc agcgaacggc gctggcgcgc gcgctggtcc
     1321 attcaccccg ggcgctgctg ctggacgagc cgttttcggc gctcgacccc gagctgcgcg
     1381 agcgcacgcg cgccgaactc gacgagctgc tccggcgcat cgacatcccg gtgctgatga
     1441 tcacgcacga cccggaagac ctcgagtggt tcggcgacga agcgctgtac ctgcgcgagg
     1501 gcgcgatcgc cgagcggccc gcaccagccc ggccgcgaga ctcgatgaaa ccatggccgc
     1561 tcgcgcccac ccactgaccc tcggcgcgga gctctggctg agcctggacg gccacaacct
     1621 cgccggggcc aggcgtgtcg cgctgctggc cgagatcgcg cgcgtcggct cgatcacgca
     1681 ggccgcgaag acggtcgggt tcagctacaa aggtgcgtgg aacgcgatcg aggacatgag
     1741 caacctggcc ggcgagccct tgctcgaccg ggtggtgggc ggcaagggcg gcggccacac
     1801 ccggctcacg cagcggggcg agaagctcgt ccacaatttc gagctgatcc ggcgggagca
     1861 tgctcgattc gtcgaccgct tgaaccggca ggcgaatggc ctgacggaag actactcatg
     1921 ggtgggcagc atcgcgttga agacaagcgc cagaaaccag tttgcaggga ccgtccgcgc
     1981 catccgcgac ggcgcgacga atgacgagat cgagctggaa gtgatcggtg gcctgcgcat
     2041 cgtcgcgacg gtgacgcgcg agagccgcgc cgagctgggg ctcgatgtcg gcgcgaaggc
     2101 cttcgcgctg gtgaaagcct cgtcgatcat gttgatgacc gacgcgggcg atgtgcgcct
     2161 gtccgcgcgc aaccagctcg cggggacggt gacccgcgtc gtgcccggcg ccgtcaacac
     2221 cgaggtcgtg ctcgaactgc ccggcggcgg gaccgtggcc gcgatcgtca ccaacgcgag
     2281 cgcccggtca ttgggcatcg gcgacggcag cccggccacc gcggtgttca aggcgtcgag
     2341 cgtgattgtc ggcgtggctg cgtgacgcag acttcgtgca ggccggactg gagcgggtga
     2401 ggggaatcga acccctgtat gaagcttggg aagctgccgt t
//
LOCUS       DATMJB010000159         3668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_216004, whole genome shotgun sequence.
ACCESSION   DATMJB010000159 DATMJB010000000
VERSION     DATMJB010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216004"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VNU48_03525"
     CDS             complement(<1..645)
                     /locus_tag="VNU48_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWI10378.1"
                     /translation="MPLSAPLVVGASFAYLLLLFAVASWGDARARAGRSVIGNGWVYA
                     LSIAVYCTAWTYFGSVGRAAGAGVWFLPIYLGPTLAMVLAWSVLRKMVRIAKTYRITS
                     IADFIASRYGKSPLLAGVVTLITVVGIVPYIALQLKAVASGYALMTAPPGMLLPAPLT
                     WWRDSTFYFALGLAGFTMVFGARHLDSAEKHEGMVAAIAFESVVKLLAFLVVGAY"
     gene            complement(661..858)
                     /locus_tag="VNU48_03530"
     CDS             complement(661..858)
                     /locus_tag="VNU48_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10379.1"
                     /translation="MHKGLAHQRLVALFGAGVLLLNFPLLALWDRDATVLGLPLFPAA
                     LFVLWGGLIAVLGWIAERLPD"
     gene            complement(888..2717)
                     /locus_tag="VNU48_03535"
     CDS             complement(888..2717)
                     /locus_tag="VNU48_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HWI10380.1"
                     /translation="MLPPLSFATAPFDGLTPAEQAQLRASAEPVRFARDAVLLTPQMR
                     AEHAWLLVEGHVQLVEAGELVASYAPGDVCAARAVLAGRASGVLSALDAVVAWQIPRA
                     TLQALIAGNAGFSAWLFGDVSRRLAASSRGREQREFLGLMMARVRDAYVRKPFYVDGA
                     LDLVSVCRLMAEQGLSNALVRDGERIGMFTTTDLRDALLRRQPPSELAVREVARFDLI
                     SIAPDAEVFEALLLMLRHRVHRVLVRERRADGSDGAILGVLGQLDLMSFVSNHSHLIA
                     LQIQQAGDVTALRAAARQMDGLVALLQGGGIRIEIICSLVRELNRQLFARLWAMLAPP
                     ELVANSCLIVMGSEGRGEQVLKTDQDNALLLRDGFELPGLAALTERFSAALIDFGYPR
                     CPGNIMLSNPLWCQPLAAFRQTLREWLYGGHADGAMNLAIFLDAAAVAGDAGLLQQAR
                     RFVDEALADNDAYLARFAGAADQFAEPAGWWARLAGLGGRDEPAFDLKKLGTFPIVHG
                     VRALALQHHVGELGTVARLRALVAGGHLAGPLARDLTDALHFLMALKLDHQLRQRGAG
                     EALDNLVTPSALGTLEGDRLKDALAIVRRFRQFLRQHYRLDAL"
     gene            complement(2827..>3668)
                     /gene="actP"
                     /locus_tag="VNU48_03540"
     CDS             complement(2827..>3668)
                     /gene="actP"
                     /locus_tag="VNU48_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059048.1"
                     /note="member of the sodium:solute symporter family;
                     cotranscribed with the acs gene which encodes acetyl
                     coenzyme A synthase; mutations affect acetate uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HWI10381.1"
                     /translation="GTAGLPHILMRFFTVPSAKEARKSVMWATGWIGYFYLLTFIIGF
                     GAITFVLTNPQFLDAKGGLLGGGNMAAIHLANAVGGNVFLGFISAVAFATILAVVAGL
                     TLSGASAVSHDLYATVIKGGRADGASELKVSRVTTVMLGILAVVLGIVFEKQNIAFMV
                     SLAFAIAASANFPVLFMSVLWKDCTTKGAVIGGFLGLISSVGLTIVSPSVWEATLGNP
                     KGSAWFPYTSPALFSMAIGFVGIWLFSMLDRSQQAGIDRGGFLAQQVRSETGIGASTA
                     SGH"
BASE COUNT          616 a         1304 c         1308 g          440 t
ORIGIN      
        1 gtaggcgccg acgaccagga acgcgagcag cttgacgacc gactcgaacg cgatcgcggc
       61 gaccatgcct tcgtgcttct cggcgctgtc gaggtggcgc gcgccgaaca ccatcgtgaa
      121 gccggccagg ccgagcgcga agtagaacgt gctgtcgcgc caccaggtca gcggcgccgg
      181 caggagcatg ccgggcggcg cggtcatcag cgcatagccg ctggccaccg ccttcagctg
      241 cagcgcgatg tagggcacga tgccgacgac ggtgatcagc gtcaccacgc cggcgagcag
      301 cgggctcttg ccgtagcggc tggcgatgaa gtcggcgatc gaggtgatgc gataggtctt
      361 ggcgatgcgc accatcttgc gcagcaccga ccacgccagc accatcgcca gcgtcgggcc
      421 gaggtagatc ggcaggaacc agacgccggc gccggcggcg cgaccgacgc tgccgaagta
      481 ggtccaggcg gtgcagtaga ccgcgatcga cagcgcgtag acccagccgt tgccgatcac
      541 cgagcggccg gcgcgcgcac gcgcatcgcc ccagctcgcg accgcgaaca gcagcagcag
      601 gtaggcgaac gacgcgccga cgaccagcgg ggcggacagc ggcatcgcag cgcccaaggc
      661 tcagtcgggc agccgctcgg cgatccagcc gagcaccgcg atcaggccac cccacagcac
      721 gaacagcgcg gccgggaaca gcggcaggcc gagcaccgtg gcgtcccggt cccacagcgc
      781 gagcagcggg aaattcagca gcagcacccc cgcgccgaac agggccacga gacgctgatg
      841 ggccaggcct ttgtgcatga gcgtgttccg cgttgagcgt cggcgcttca gagcgcgtcg
      901 agccggtagt gctggcgcag gaactggcgg aagcgccgga cgatcgccag tgcgtccttc
      961 agccgatcgc cttcgagcgt tcccagcgca gacggcgtca ccaggttgtc gagtgcctcg
     1021 ccggcgccgc gctggcgcag ctggtgatcg agcttcagcg ccatcaggaa gtgcagcgcg
     1081 tcggtcaggt cgcgcgccag cggcccggcc aggtgaccgc ccgcgaccag cgcacgcagc
     1141 cgcgcgaccg tgccgagctc gccgacgtgg tgctgcagcg cgagcgcgcg cacgccgtgg
     1201 acgatcggaa aggtgccgag cttcttcagg tcgaaggccg gttcgtcccg cccgcccagg
     1261 ccggcgagcc gcgcccacca gccggccggt tcggcgaact ggtcggccgc gcccgcgaag
     1321 cgcgcgaggt aggcgtcgtt gtcggccagc gcctcgtcga cgaagcgccg cgcctgctgc
     1381 agcaacccgg cgtcgccggc gaccgcggcg gcatcgagaa agatcgccag gttcatcgcg
     1441 ccgtcggcgt ggccgccgta cagccactcg cgcagcgtct ggcggaacgc cgccagcggc
     1501 tggcaccaca gcgggttgct gagcatgatg ttgcccgggc agcgcgggta gccgaagtcg
     1561 atcagcgcgg cgctgaagcg ctcggtcagc gcggccaggc cgggcagctc gaagccgtcg
     1621 cgcagcagca gcgcgttgtc ctggtcggtc ttcagcacct gctcgccgcg gccttcgctg
     1681 cccatcacga tcaggcagct gttggccacc agctcgggcg gcgccagcat cgcccacagc
     1741 cgcgcgaaca gctgccggtt gagttcgcgc accaggctgc agatgatctc gatgcggatg
     1801 ccgccaccct gcagcagcgc gaccagcccg tccatctgcc gcgccgcggc acgcagcgcg
     1861 gtcacgtcac cggcctgctg gatctgcagc gcgatcaggt gcgagtggtt cgagacgaag
     1921 ctcatcaggt cgagttggcc gagcacgccg aggatcgcgc cgtcggaacc atcggcacgg
     1981 cgctcgcgca ccagcacgcg gtgcacgcga tggcgcagca tcaacagcag cgcctcgaag
     2041 acctcggcat cgggcgcgat gctgatcagg tcgaagcgcg cgacctcgcg caccgcgagc
     2101 tcgctcggcg gctgccgccg cagcagcgcg tcgcgcaggt cggtggtggt gaacatgccg
     2161 atgcgctcgc cgtcgcgcac cagcgcgttg ctcaggccct gctcggccat cagccggcag
     2221 accgagacga ggtcgagcgc gccgtcgaca tagaacggct tgcgcacata ggcatcgcgc
     2281 acgcgggcca tcatcaggcc gaggaattcg cgctgctcgc gcccgcgcga cgaggccgcg
     2341 agccggcgcg agacatcgcc gaacagccag gcgctgaagc cggcgttgcc ggcgatcagc
     2401 gcctgcaacg tggcgcgcgg gatctgccag gcgacgaccg cgtcgagcgc gctcagcacg
     2461 ccgctggcgc ggccggcgag caccgcgcgc gcggcgcaca catcgcccgg cgcgtagctc
     2521 gcgacgagct cgccggcctc gaccagctgg acgtggccct cgaccagcag ccaggcgtgc
     2581 tcggcccgca tctgcggcgt cagcagcacc gcgtcgcggg cgaagcgcac cggctcggcg
     2641 ctggcacgca gctgcgcctg ctcggccggc gtcaggccgt cgaacggtgc ggtggcgaag
     2701 ctcagtggcg gcagcacggc gggaggggtt cacgtggcga cgggactgcc gacagtgtgc
     2761 ccggaggcgc cgcccgcaat cccgtcagtg caacgggcgg cacggcggct gccgcaccgc
     2821 gcgggctcag tgccccgacg ccgtcgaggc gccgatgccg gtctccgaac gcacctgctg
     2881 ggccaggaag ccgccgcggt cgatgccggc ctgctggctg cggtcgagca tcgagaacag
     2941 ccagatgccg acgaaaccga tcgccatcga gaatagcgcg ggcgaggtgt acgggaacca
     3001 cgccgagccc ttcggattgc ccagcgtcgc ctcccacacc gacggcgaga cgatcgtcag
     3061 gccgaccgac gagatcagcc cgaggaagcc gccgatcacc gcgcccttgg tggtgcagtc
     3121 cttccacagc acgctcatga agagcaccgg gaagttcgcc gaggccgcga tcgcgaaggc
     3181 cagcgacacc atgaaggcga tgttctgctt ctcgaacacg atgccgagca cgaccgcgag
     3241 gatgcccagc atgaccgtcg tgacgcgcga gaccttcagc tccgaagcgc cgtcggcgcg
     3301 gccgcccttg atcacggtcg cgtacaggtc gtgcgagacc gccgacgcgc cggacagcgt
     3361 caggccggcc accaccgcga ggatggtcgc gaacgccact gccgagatga agccgaggaa
     3421 gacgttgccg ccgaccgcgt tggcgaggtg gatcgccgcc atgttgccgc cgccgagcag
     3481 cccgcccttg gcgtcgagga actgcgggtt ggtcagcacg aaggtgatcg cgccgaagcc
     3541 gatgatgaag gtcagcaggt agaagtagcc gatccagccg gtcgcccaca tcaccgactt
     3601 gcgcgcctcc ttggcgctgg gcaccgtgaa gaagcgcatc aggatgtgcg gcagcccggc
     3661 ggtgccga
//
LOCUS       DATMJB010000160         5560 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_756756, whole genome shotgun sequence.
ACCESSION   DATMJB010000160 DATMJB010000000
VERSION     DATMJB010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756756"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VNU48_03545"
     CDS             <1..309
                     /locus_tag="VNU48_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HWI10382.1"
                     /translation="GGDDSVTRFFAAKTRAPLSVTFLPIVTRATEKVALADKYNAVAG
                     KAASLGLMKKDDANVQQYVTGKTLDGLYLMIGEEERKIRQNPVATGSAILSKVFGSLK
                     "
     gene            complement(332..1480)
                     /locus_tag="VNU48_03550"
     CDS             complement(332..1480)
                     /locus_tag="VNU48_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10383.1"
                     /translation="MSDTGRNPAAHGVAHTLPGDGSESEHQRWHELVSQIGAEIAAPL
                     TAALERINSLTSTGRIDRQGLRALREEVEAARQVGMIGQQLTRFASGRLRQSHERMQL
                     ADVLNGVIAHRMRQTTARGISLKPSLKPAEVIVDGSLLFSVLNSTLDWALANAHSQID
                     FTIDIKSWPAHALLTCRFAHRPVDQMTDGAGATVPHGLDSLTWRLLEQTAWTMGLVVE
                     RHDEAHLTTLTLEFPRTASSEIEGVSTMEIDDGFAPSNNSQPLAGSHVLVVAARRELR
                     AQIRDALRDMSLIIDFVNSVDEAAAFCREGLPHAVIIESIQTGERFAQLREEIGAEVP
                     DFVFIEIVEEGTIFEMSGFSDSTMARVGRGALASSLPSALMFELSKGL"
     gene            complement(1757..3037)
                     /locus_tag="VNU48_03555"
     CDS             complement(1757..3037)
                     /locus_tag="VNU48_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWI10384.1"
                     /translation="MASSATPSPQALSGVRVLDLSRVLAGPWCTQTLADLGADVIKVE
                     RPGSIAADGRRSGGDDTRGWGPPFLKDRAGADTLEAAYYVGTNRNKRSITVDIARPEG
                     QALIRRIALWSDVFVENYKVGDMARYGLDFARLSALNPRLVYCSITGFGQTGPYCERA
                     GYDYAIQGMGGLMSVTGPSRAEIADDASGGGPQKVGVAVADLFTGMYATTAILAALRH
                     RDLTGEGQAIDMALLDTQVAMLANLGANYLTTGIAPQRMGNAHQNIVPYQVFEVADGH
                     MIVAVGNDGQFAKFCEVAGRPEVANDARFARNADRVRHRAVLVPLLAAWMKTRDRADW
                     LEALEAAKVPCGPINDLADVFADPQVRQREMTVQLAHPLAGSVRLVASPMKFSGTPVQ
                     YRRPPLLGEHTAELLREFGLDDAEIAALRAAHAI"
     gene            3074..3601
                     /locus_tag="VNU48_03560"
     CDS             3074..3601
                     /locus_tag="VNU48_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10385.1"
                     /translation="MTKSAFRTAALAAAAIGAACAPTLDWREFVPEGSDISVRFPCRP
                     DRSARSVTIAGATVQMQMLACSAGNATFALAFVEVADPARIGATLAALRDTAVANVHA
                     GEPRSAALQIQGMTPNEQSGRLAVTGRLPDGAAVQEHAVFFTRGLRVYQAAVIGAAPA
                     PEAVETFIGGLKFPG"
     gene            3852..4289
                     /locus_tag="VNU48_03565"
     CDS             3852..4289
                     /locus_tag="VNU48_03565"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HWI10386.1"
                     /translation="MIIAHAPLASSLKSVAEHTFPDCSRLLEALDVAPDMPIEEVEAR
                     AREMLARVRNPEALIFTDVFGATPCNVAQRLGDGAQVRVIAGVNVPMLWRSLCYATEP
                     LEAVVMRAMAGATQGVMQVATSKPQNQTLKPGGNDQAKHHHQQ"
     gene            4258..4527
                     /locus_tag="VNU48_03570"
     CDS             4258..4527
                     /locus_tag="VNU48_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010101417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HWI10387.1"
                     /translation="MIKQSITISNKLGLHARASAKLTKLAGSFHSDVFMSRNERRVNA
                     KSIMGVMMLAAGVGSVIDIEVTGPDEQAAMAALIALINDKFGEGQ"
     gene            4631..>5560
                     /locus_tag="VNU48_03575"
     CDS             4631..>5560
                     /locus_tag="VNU48_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HWI10388.1"
                     /translation="MSFQVFGLPVSRGVAIGRAVLVASSRVDVAHYFIDAERVEAEVA
                     RLRSARDAVANELSTLQRELPAEAPAELSALLDVHLMLLHDETLTGAASQWIHERHYN
                     AEWALSAQLEVLARQFDEMEDEYLRERKADLEQVVERLLRAMARGPASSFAPAPGVRA
                     RDFAGEDPLVLVANDIAPADMLQFKRSVFTGFITDVGGRTSHTAIVARSMDIPAVVGA
                     REASRIIRQDDWIVIDGDAGIVIVDPSPIVLEEYRFRQRQIELGRDRLARLRHTPAVT
                     TDGERVELLANIEMPSDAVAALEAGAVGVGLFRS"
BASE COUNT          828 a         1847 c         1994 g          891 t
ORIGIN      
        1 ggtggcgacg actcggtgac gcgattcttc gcggcaaaga cgcgcgcgcc gctgtcggtg
       61 accttcctgc ctatcgtcac ccgggcgacc gagaaggtgg cgttggccga caagtacaac
      121 gcggtcgccg gcaaggccgc ctcgctcggc ctgatgaaga aggacgacgc caatgtccag
      181 cagtacgtca caggcaagac gcttgacggc ctgtacctga tgatcggcga ggaagaacgc
      241 aagatccgcc agaacccggt ggccaccggc agcgcgatcc tgtcgaaggt gttcggctcg
      301 ctgaagtagt gcgcgagatc gggggcgccg ctcagagccc cttcgacagc tcgaacatca
      361 gcgccgacgg cagcgaactc gcgagcgccc cgcgcccgac gcgcgccatc gtgctgtcgc
      421 tgaagcccga catctcgaag atcgtgccct cttcgacgat ctcgatgaag acgaaatcgg
      481 gcacctcggc gccgatctcc tcgcgcagct gcgcgaagcg ctcgccggtc tggatcgatt
      541 cgatgatcac ggcgtgcggc aggccttcgc gacagaacgc ggcggcctcg tcgaccgagt
      601 tgacgaagtc gatgatcagg ctcatgtcgc gcagcgcatc gcggatctgc gcccgcagtt
      661 cgcgtcgcgc cgcgaccacg agcacgtggc tgccggccag cggctgggag ttgttcgacg
      721 gcgcgaagcc gtcgtcgatc tccatcgtgc tgacgccttc gatctcgctg ctcgcggtgc
      781 gcgggaactc cagcgtcagc gtcgtcaggt gcgcctcgtc gtggcgttcg acaaccaggc
      841 ccatggtcca cgcggtctgc tcgagcagcc gccacgtgag cgagtcgagg ccatgcggca
      901 cggttgcgcc ggcgccgtcg gtcatctggt cgaccgggcg gtgtgcgaaa cggcaggtca
      961 gcagcgcgtg cgccggccag ctcttgatgt cgatcgtgaa atcgatctgc gagtgggcgt
     1021 tggccagcgc ccagtcgagc gtcgaattca gcacgctgaa cagcagcgag ccatcgacga
     1081 tcacctcggc cggtttcagc gacggcttca ggctgatgcc gcgtgccgtc gtctggcgca
     1141 tccggtgcgc gatcacgccg ttcagcacgt cggccaattg catccgctcg tgcgactggc
     1201 gcagccgccc ggaggcgaag cgcgtcagct gctggccgat catgccgacc tggcgtgcgg
     1261 cctcgacctc ttcgcgcagc gcgcgcaggc cctggcggtc gatccgaccg gtgctggtca
     1321 ggctgttgat gcgctcgagc gcggcggtca gcggcgcggc gatctcggcg ccgatctgcg
     1381 acaccagctc gtgccagcgt tggtgttcgc tttccgaccc gtcaccgggc aaggtgtgcg
     1441 caaccccgtg cgcggccggg ttgcgaccgg tgtcagacat gtccacgctc acttcctctt
     1501 cttgtgttcg cgccgcggcg cggagtgcgg gccatcgggg cgcccgtatg ccgtccgtgc
     1561 gtcgaacggg gtgccgtcgg ccatgcgagc gagtgtcggc tgcggcggcg ccaggcgcca
     1621 gggcattgag ccgcccgtcc ccgcacatgg ggggtgaaat ggtcacgttc cggggcaccg
     1681 gatcacgggg tgcgcgagcg cgtcgccgtc gagatcgcgc gagggcccga acgcggcgag
     1741 cagcggctgc ggcgcgtcag atcgcgtggg ccgcgcgcag cgccgcgatc tcggcgtcgt
     1801 cgaggccgaa ttcgcgcagc agttcggccg tgtgttcgcc cagcagcggc ggccgccggt
     1861 actgcaccgg cgtgccggag aacttcattg ggctggccac gaggcgcacg ctgccggcca
     1921 gcgggtgcgc cagctgcacg gtcatctcgc gctgccgcac ctgcgggtcg gcgaacacgt
     1981 ctgccaggtc gttgatcggg ccgcacggca ccttcgcggc ttccagcgct tcgagccagt
     2041 cggcgcgatc gcgcgtcttc atccacgcgg ccagcagcgg caccagcacg gcacggtggc
     2101 gcacgcgatc ggcgttgcgg gcgaagcgcg cgtcgttcgc gacttcggga cggccggcga
     2161 cctcgcagaa cttcgcgaac tggccgtcgt tgccgaccgc gacgatcatg tgcccgtcgg
     2221 cgacctcgaa tacctggtag ggcacgatgt tctggtgcgc attgcccatg cgctgcggtg
     2281 cgatgcccgt ggtcaggtag ttcgcgccca ggttcgcgag catcgcgacc tgggtgtcca
     2341 gcagcgccat gtcgatggcc tggccttcgc cggtgagatc gcgatggcgc agcgcggcca
     2401 ggatcgcggt ggtcgcgtac atgccggtga acaggtcggc gaccgcgacg ccgaccttct
     2461 gcgggccgcc gcccgacgcg tcgtcggcga tctcggcccg cgacgggccg gtcacgctca
     2521 tcaggccgcc catgccctgg atcgcgtagt cgtagccggc gcgttcgcag tacggcccag
     2581 tctgaccgaa gccggtgatc gagcagtaga ccaggcgggg gttcagcgcg ctcaggcggg
     2641 cgaaatcgag gccgtagcgc gccatgtcgc cgaccttgta gttctcgacg aaaacgtcgc
     2701 tccacagagc gatgcggcgg atcagcgcct ggccttcggg ccgcgcgatg tcgaccgtga
     2761 tcgagcgctt gttgcggttc gtgccgacgt agtaggcggc ctcgagggtg tcggcgccgg
     2821 cccggtcctt caggaacggc gggccccagc cgcgggtgtc gtcgccgccg ctgcgacggc
     2881 cgtcggcggc gatgctgccc gggcgctcga ccttgatcac gtcggcaccg aggtcggcca
     2941 gcgtctgcgt gcaccacggg ccggcgagca cgcgcgacag gtcgagcacg cgcacgcccg
     3001 acagcgcttg tggcgaaggg gtcgcgctgc ttgccatccg gtcattgtga agcagcgccg
     3061 ggataatgcg gcgatgacca agtcggcctt tcggacggcg gcgcttgccg cggccgcgat
     3121 cggcgccgcg tgcgcgccga cactcgactg gcgcgaattc gtgcccgaag gcagcgacat
     3181 cagcgtgcgc tttccgtgcc gaccggaccg cagcgcccga tcggtgacga tcgccggcgc
     3241 aacggtgcag atgcagatgc tggcgtgcag tgcgggcaac gcgaccttcg cgctcgcgtt
     3301 cgtcgaggtc gccgacccgg cgcgcatcgg cgccacgctg gccgcgctgc gcgacactgc
     3361 cgtcgccaat gtgcacgcag gcgagccgcg cagcgcggcg ctgcagatcc agggcatgac
     3421 gccgaacgag cagtcggggc gcctggcggt gacgggccgg ctgccggacg gcgcggcggt
     3481 gcaggagcat gccgtctttt tcacccgcgg cctgcgcgtc taccaggccg cggtgatcgg
     3541 cgccgctccg gcgccggaag cagtcgagac cttcatcggc gggctgaagt ttcccggatg
     3601 agcgcggtcg cgcttccacc acggcggtct gatcggcgcg ttctgcctgc aatgggcatc
     3661 ggcctgtcga tcgacctgtt gcgcgcatgg ggatggggca cgttgtcggc ctgccgaggt
     3721 ggttttgcgt tgttgtgggg ctgccgtctg ttttcgtatg tgtggttttt gtgtcgggtc
     3781 gagcccgcga agcctgcgaa actgaactcg agcgcatgaa ctgtcgataa tttgttgcca
     3841 tgcccggatt gatgatcatt gcccacgcgc cgctcgcgtc ttcgctgaaa tcggtcgccg
     3901 agcacacgtt ccccgactgc tcgcggctgc tggaagcgct cgacgtggcg cccgacatgc
     3961 cgatcgagga ggtcgaggcg cgggcccgcg agatgctcgc ccgtgtgcgc aaccccgagg
     4021 cgctgatctt cacggatgtc ttcggcgcga cgccctgcaa tgtggcgcag cgtctcggcg
     4081 atggtgcgca ggtacgcgtg atcgccggcg tcaacgtgcc gatgctgtgg cgctcgctat
     4141 gctatgcaac cgaaccgctc gaagcggtcg tgatgcgggc gatggccggt gcgacccagg
     4201 gcgtgatgca ggtggcgacc tcgaagccgc agaatcagac cctcaagccg ggcggaaatg
     4261 atcaagcaaa gcatcaccat cagcaataag ctgggtcttc atgcccgcgc atcggcgaag
     4321 ctgacgaagc tggccggcag ttttcacagc gacgtcttca tgagccgcaa cgaacgccgc
     4381 gtcaacgcca agagcatcat gggcgtgatg atgctggcag cgggggtcgg cagcgtgatc
     4441 gatatcgagg tgacgggccc cgacgagcag gccgcgatgg ccgcgctgat cgcgctgatc
     4501 aacgacaagt tcggcgaagg ccagtaggcg cccagcgctg cgcgcccggg aagtggcgac
     4561 ggtggcgcgc cgccaggtcg tcgaagcgca gcagcatcgc tagggtgccg gcgctgctag
     4621 catcggacgc atgagcttcc aggtcttcgg cttgccggtg tcacgcggcg tcgcgatcgg
     4681 gcgggcggtg ctcgtcgcgt cgagccgcgt ggatgtcgcg cactacttca tcgacgcgga
     4741 gcgcgtcgag gccgaagtgg cgcgcctgcg cagcgcgcgc gatgcggtcg cgaacgaact
     4801 cagcaccttg cagcgtgagc tgccggccga ggcgccggcg gaactctcgg cactgctcga
     4861 cgtccacctg atgctgctgc acgacgagac actcaccggt gccgccagcc agtggattca
     4921 cgagcggcac tacaacgccg aatgggcgct gtcggcgcag ctcgaggtgc tggcgcgtca
     4981 gttcgacgag atggaggacg agtacctgcg cgagcgcaag gccgatctag agcaggtcgt
     5041 cgagcggctg ctgcgggcga tggcgcgtgg gccggcgtct tcgttcgcac ccgcgccggg
     5101 ggttcgcgcg cgcgatttcg ccggcgagga tccgctggtg ctggtcgcga acgacatcgc
     5161 gccggccgac atgctgcagt tcaagcgcag cgtgttcacc ggcttcatca ccgacgtcgg
     5221 cggcagaacg tcgcacaccg cgatcgtcgc gcgcagcatg gacattcccg cggtggtggg
     5281 cgcgcgcgag gcgagccgca tcattcgcca ggacgactgg atcgtgatcg acggcgacgc
     5341 cggtatcgtc atcgtcgacc cctcgccgat cgtgctggag gaataccgct tccgccagcg
     5401 ccagatagag ctcggacgcg accgcctggc gcggctgcgc cacacgccgg ccgtcacgac
     5461 tgatggcgag cgggtcgaac tgctcgcaaa catcgagatg ccgagcgatg ccgtcgccgc
     5521 gctggaggcg ggcgccgtcg gcgtcgggct gttccgcagc
//
LOCUS       DATMJB010000161         3151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_156865, whole genome shotgun sequence.
ACCESSION   DATMJB010000161 DATMJB010000000
VERSION     DATMJB010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156865"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VNU48_03580"
     CDS             <1..202
                     /locus_tag="VNU48_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWI10389.1"
                     /translation="ALEPAAFAARVAEAARQDPRTEVQLRADTGVPYGRVAELIGVVQ
                     KAGLNRIGFVTEPGAAASGSHP"
     gene            complement(297..1265)
                     /locus_tag="VNU48_03585"
     CDS             complement(297..1265)
                     /locus_tag="VNU48_03585"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HWI10390.1"
                     /translation="MLQFADIHAAAARLKGVAHRTPVLTSRTLDAMLGMQVFMKAENL
                     QRMGAFKFRGGYNAVSVLSDAQRARGVVAFSSGNHAQAVALAAQLHGCRATIVMPHDA
                     PALKLAATRGYGAEVVLYDRYQEDRAAIATRLVEQRGAALIPPFDHLPVMAGQGTAAL
                     ELIEDVGALDALIVCAGGGGFLSGCAVAARQLLPQVRLYGAEPERGNDMQQSLRAGRI
                     VAIEVPRTICDGQQTQAVGAHPFEVIRALVTDVLTATDPAVVDAMRFAFERMKVVLEP
                     SGACALAALMTHAEQFKGQRVGITLSGGNIGLDRFVALVSGAEKVD"
     gene            1435..1725
                     /locus_tag="VNU48_03590"
     CDS             1435..1725
                     /locus_tag="VNU48_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HWI10391.1"
                     /translation="MATYITLANFTDQGARAIKGSPDRFEAFKALAESSGVSVKSAHW
                     TLGAYDLVLVAEGTEEAVAALNMKTAALGNVRTQTLRGFTPAEMRKLVAGLG"
     gene            complement(1682..2323)
                     /locus_tag="VNU48_03595"
     CDS             complement(1682..2323)
                     /locus_tag="VNU48_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HWI10392.1"
                     /translation="MKPSTSAPPPQAEALIRHTRAWVERAVVGLNLCPFAKAPLVKGL
                     VHFSVCESDDPRVLLEVLCAEMTALVQADPQQRETTLLIHPNVLEDFDAFNDFLDAAD
                     AALEELGLEGVLQVASFHPRYRFADATADAIGNATNRAPYPTLHLLREASVERAVAAF
                     PAAETIFEANVATLEALGAAGWAALQAQCRADAAGAGPGVSPGPPPACASRPV"
     gene            complement(2333..>3151)
                     /locus_tag="VNU48_03600"
     CDS             complement(2333..>3151)
                     /locus_tag="VNU48_03600"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006592.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HWI10393.1"
                     /translation="LTLLAATAPWPAAHAAAPALPLLGVWSDVLDPARYLVSEKYDGV
                     RGVWDGMTLRFRSGRVVPAPAWFTAQLPRVPLDGELWLGRGRFDEVSGIVRKAVPVDA
                     EWRRLRYMVFELPGAPGSFAERATRIEAIVGRSAAPALVAVRQTTLGDRAALQRALAA
                     TIAQGGEGLVLHLASAPASIGRSDAILKLKPSLDAEATVIGHRAGKGKYEGLLGALEL
                     RTPQGRRFLIGSGLSDALRREPPAIGSVVTYRYRDPTGTGLPRFASFMRVHDAL"
BASE COUNT          453 a         1139 c         1136 g          423 t
ORIGIN      
        1 ggcgctcgag cccgcggcct tcgccgcccg cgtcgccgag gcggcgcgcc aggacccgcg
       61 gaccgaggtc cagctgcgcg ccgacaccgg cgtgccctac ggccgcgtcg ccgagctgat
      121 cggcgtggtg cagaaggccg ggctgaaccg gatcggcttc gtcaccgaac ccggcgcggc
      181 cgccagcggc agccacccct gaaacgttcg gggcggcgcg cgcgttccaa gaacatgccg
      241 ttgcggaggt cggcctcaac accgtcaacg tgctcgacgc cttcgacgcc gcatagtcag
      301 tcgaccttct cggcgcccga gacgagcgcg acgaagcggt ccaggccgat gttgccgccc
      361 gacagcgtga tgccgacgcg ctgacccttg aactgctcgg catgcgtcat cagcgcggcg
      421 agcgcgcagg cgcccgaggg ttccagcacg accttcatgc gttcgaaggc gaagcgcatc
      481 gcgtcgacga cggcggggtc ggtggccgtc agcacgtcgg tgacgagcgc acggatcacc
      541 tcgaacggat gcgcgccgac ggcctgcgtc tgctggccgt cgcagatcgt gcgcggcacc
      601 tcgatcgcga cgatgcgccc ggcgcgcagc gactgctgca tgtcgttgcc gcgctcgggc
      661 tcggcaccgt acaggcggac ctgcggcagc agctgcctgg ccgcgacggc gcagcccgac
      721 aggaagccgc cgccgccggc gcagacgatc agcgcgtcga gtgcgcccac gtcctcgatc
      781 agttcgagcg ccgcggtgcc ctgcccggcc atcaccggca ggtggtcgaa cggcgggatc
      841 agcgcggcgc cgcgctgctc gaccagccgg gtcgcgatcg cggcgcggtc ctcctggtag
      901 cggtcgtaga gcacgacctc ggcgccgtag ccgcgcgtcg ccgcgagctt cagcgccggc
      961 gcgtcgtgcg gcatcacgat ggtcgcgcgg caaccgtgca gctgcgccgc gagcgcgacc
     1021 gcctgcgcgt ggttgcccga cgagaacgcg accacgccgc gcgcgcgctg cgcgtcgctg
     1081 agcacgctca ccgcgttgta gccgccgcgg aacttgaacg cgcccatgcg ctgcaggttc
     1141 tccgccttca tgaagacctg catgcccagc atcgcgtcga gcgtgcgcga ggtcagcacc
     1201 ggggtgcggt gggcgacgcc cttcaggcgg gcggccgcgg cgtggatgtc ggcgaattgc
     1261 agcatgggat cgagaatacg ctgccttgat attggcgggc cgcggcctag actttggcct
     1321 gtgcgcaggg ccgcatccct tgcgcgatgg cgccgagacc cgacggccgg tctcgatccg
     1381 catgcagccc gtggccgtcg gttcacccac ccgaacgcat cgaggagact tgcgatggcc
     1441 acctacatca cgctggcgaa cttcaccgat cagggcgcac gcgccatcaa ggggtcgccc
     1501 gaccgtttcg aggccttcaa ggcgctggcc gaatccagcg gcgtgtccgt caagagcgcg
     1561 cactggacgc tcggcgccta tgacctcgtg ctggtcgccg aaggcaccga agaagccgtg
     1621 gccgccctga acatgaagac ggccgcactc ggcaacgtgc gcacgcagac cctgcgtggc
     1681 ttcacaccgg ccgagatgcg caagctggtg gcgggcctgg gctgaccccc ggcccggcgc
     1741 cggcggcatc ggcccggcat tgcgcctgca gcgccgccca gccggcggcg cccagcgctt
     1801 ccagcgtcgc gacattggct tcgaagatcg tctcggccgc ggggaacgcg gccaccgcgc
     1861 gctcgacact ggcttcgcgc agcaggtgca gcgtcgggta gggcgcgcgg ttggtcgcat
     1921 tgccgatggc gtcggccgtg gcgtcggcga agcgatagcg cgggtgaaag ctcgcgacct
     1981 gcagcacgcc ctcgaggccg agttcttcga gcgcggcgtc agcggcgtcc aggaaatcgt
     2041 tgaacgcgtc gaagtcctcg agcacgttcg ggtggatcag cagcgtcgtc tcgcgctgct
     2101 gcgggtcagc ctgcaccagc gcggtcattt cggcgcacag cacctcgagc agcacgcgcg
     2161 gatcgtcgct ctcgcagacc gagaaatgga ccagcccttt caccagcggc gccttcgcga
     2221 acgggcacag gttcaggccg acgaccgcgc gctcgaccca ggcgcgcgtg tggcggatca
     2281 gcgcctcggc ctggggcggc ggcgcagacg tcgagggctt catggcagcc ggttatagcg
     2341 catcgtgcac gcgcatgaag ctcgcgaagc gcggcagccc ggtgcccgtc gggtcgcggt
     2401 agcggtaggt gacgacgctg ccgatcgccg gcggctcgcg ccgcaacgcg tcgctcaggc
     2461 cgctgccgat caggaagcgc cggccttgcg gcgtgcgcag ttcgagcgcg ccgagcagcc
     2521 cttcgtactt gcccttgccg gcacgatgac cgatcaccgt ggcctcggcg tcgaggctgg
     2581 gcttgagctt gaggatcgcg tcgctgcggc cgatgctcgc gggcgcggag gcgaggtgca
     2641 gcaccaggcc ttcgccacct tgagcgatcg ttgcggccag tgcgcgctgc agcgccgcgc
     2701 ggtcgcccag cgtcgtttgc cggaccgcga ccagcgccgg cgccgcgctg cggccgacga
     2761 tcgcctcgat gcgcgtcgcg cgctcggcga agctgcccgg cgcgcccggc agttcgaaga
     2821 ccatgtagcg cagccggcgc cattcggcat cgaccggcac ggccttgcgc acgatgcccg
     2881 agacctcgtc gaagcggccg cgcccgagcc acagctcgcc gtcgagcggc acgcgcggca
     2941 gctgggcggt gaaccaggcc ggcgccggca cgacgcggcc gctgcgaaag cgcagggtca
     3001 tgccgtccca cacgccgcgc acgccgtcgt acttctcgct gacgaggtag cgcgccgggt
     3061 cgagcacgtc gctccagaca ccgagcagcg gcagcgccgg tgcggccgca tgcgccgccg
     3121 gccagggcgc cgtcgcggcg agcagggtca a
//
LOCUS       DATMJB010000162         2941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_97155, whole genome shotgun sequence.
ACCESSION   DATMJB010000162 DATMJB010000000
VERSION     DATMJB010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97155"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..605
                     /locus_tag="VNU48_03605"
                     /pseudo
     CDS             303..605
                     /locus_tag="VNU48_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
     gene            753..1895
                     /locus_tag="VNU48_03610"
     CDS             753..1895
                     /locus_tag="VNU48_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI10394.1"
                     /translation="MAAHLVGKFEVLRHRGGAGDEEAYPALRKEWELELARGGWTCVG
                     WPREVGGRGLSIAHQIIFHEEYARAGGPGHLSHLSETLLGPTVIAYGTDDQKRRFLPG
                     IVAGTDYWCQGYSEPGAGSDLANVQTRARFDEAAQEWIIDGQKVWTSLAHECQYIFVV
                     ARSEPGSRLHKGLIFLLVDLRQPGVEVRPIRQITGTCEFNEVFFDGARCPAANVIGAP
                     GEGWKIAMALLGFERGLSWIGMQQHFEHQLELVTKAARETGASRNPALRERIGRAWMG
                     LKVMHYITLRTLAGGDDGSLSREGYIVKHYWSNWHRDLGELAMDVLGVEGDIQIDDFA
                     RTRLQQVWLFSRSDTIVAGTNEIQLNIIAERALGMPKDPRPDLKAA"
     gene            1892..2683
                     /locus_tag="VNU48_03615"
     CDS             1892..2683
                     /locus_tag="VNU48_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI10395.1"
                     /translation="MNPQPIPPYVGGHRLLAGKSALVTACAGGGIGFATAKRFAEEGV
                     RALFMSDIHARRLDEAVARIKAETGFETIHCRVCDVTQQDQVDALVNEADDVLSGTDI
                     LFNNAGLGGQKALVEMTDGEWLRVIDVSLNGTFRMTRAMMKKMIPRGKGAIINNSSVL
                     GWRAQKEQAHYAAAKAGVNALTRCAALEAAEHGIRINAVSPSIAMHDFLKKTADEGLL
                     ARLAEREAFGRAAEVWEMSNIVVFLASDYASYLVGEIIAASSQHA"
     gene            2700..>2941
                     /locus_tag="VNU48_03620"
     CDS             2700..>2941
                     /locus_tag="VNU48_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HWI10396.1"
                     /translation="MATVISSATQLAGLVGQELGVTDWLEVTQERVNQFADATGDHQW
                     IHVDPARAAGGPFGGCVAHGYLSLALAGSYFLERIV"
BASE COUNT          484 a         1003 c         1018 g          436 t
ORIGIN      
        1 tgcccgaggg cgctcacgtc gatctgctgc tcgtgcccgc gcgtttggac ggtgcggcgg
       61 gcgatgccgc tctggccttg ttcgccgtgc cggggacggc cgccggggtg cgccggcaca
      121 atcatgccgg cttcgacggt acccgccgcg tggccgaggt gagcctgacg gcggtgcgcc
      181 tggagcccgc tgccttgctt gccagcggcg cccgcctggc cgatggcctg gaacgcactg
      241 ccgcgctcgc cgcgatcgcg ctgtcggccg aacaactggg tggcgcgcag caatgcctgg
      301 acatgtcgtt ggcctatgcc gccgaccgcg tgcagttcgg gcgcaagatc aaccagtacc
      361 aggcggtcaa gcaccgctgc gccgagatga tgttgcgcat cgaagccgcc gcctcggcgc
      421 tggccggtgc ggtgcgcttg attgcgctga agcccgacac cgccacgctg cggcgcgagg
      481 cggcctgcgt caagaccttc gcgtcggaca ccttccgcta ctgttcaggc gaagccatcc
      541 agctgcacgg cggcgtgggg ttcacctggg agtacgaccc gcacctgtac ttcaagcgcg
      601 cgcaggccgg ctcgcagtgg ctgggcgcct ccagcgtgct gcgcgaacgc atcgcgcggg
      661 caattttcga cggcccgccc ggcagcgaac cgagtgccca gccacctgcc ggctcgggcg
      721 acgaagcctt tcgcgccgac gtgcggcgct ggatggcggc gcacctggtc ggcaagttcg
      781 aggtgctgcg ccaccgcggc ggcgcgggag acgaagaggc ctacccggca ctgcgcaagg
      841 agtgggaact cgaactcgcg cgcggcggct ggacctgcgt gggctggccc agggaagtgg
      901 gcgggcgcgg cctctcgatc gcgcaccaga tcatcttcca tgaggaatac gcacgcgccg
      961 gcggccccgg acacctgagc cacctcagcg aaaccctgct cgggcccacg gtgattgcgt
     1021 atggcaccga cgaccagaag cggcgctttc tgcccggcat cgtggccggc accgactact
     1081 ggtgccaggg ctacagcgag ccgggtgccg gttccgacct ggccaatgtg cagacccgtg
     1141 cacgcttcga cgaagccgcg caagagtgga tcatcgacgg ccagaaggtc tggacctcgc
     1201 tcgcgcacga gtgccagtac atcttcgtcg tggcgcgcag cgagcccggc tccaggctgc
     1261 acaagggcct gatcttcctg cttgtcgatc tgcgccagcc cggtgtcgaa gtgcgcccga
     1321 tccgccagat caccgggacc tgcgaattca acgaggtgtt cttcgatggc gcacgctgcc
     1381 cggccgccaa tgtgatcggg gcgccgggtg aaggctggaa gatcgcgatg gcgctgctcg
     1441 gcttcgagcg cgggctgtcg tggatcggca tgcagcaaca cttcgagcac cagctcgaac
     1501 tggtcacgaa agctgcgcgc gagaccggtg ccagccgcaa cccggcgctg cgcgagcgca
     1561 tcggccgggc ctggatgggc ttgaaggtga tgcactacat caccctgcgc acgctggccg
     1621 gcggcgacga cggcagcctt tcgcgcgagg gctacatcgt caagcactac tggtcgaact
     1681 ggcaccggga tctgggcgag ctggcgatgg acgtgctcgg tgtcgaaggc gacatccaga
     1741 tcgacgactt cgcgcgcacg cggctgcagc aggtctggct gttcagccgc tccgacacca
     1801 tcgtggccgg caccaacgag atccagctca acatcatcgc cgagcgcgcg ctgggcatgc
     1861 ccaaagaccc ccgtcccgac ctgaaggccg catgaaccct caacccattc ccccctacgt
     1921 gggcggccac cggctgctcg ccggcaagtc ggccctggtc accgcatgcg cgggtggcgg
     1981 catcggattc gccaccgcca agcgattcgc ggaagagggc gtgcgcgccc tgttcatgtc
     2041 cgacatccac gcgaggcggc tcgacgaggc ggtggccagg atcaaggccg agaccgggtt
     2101 cgagaccatt cactgccgcg tgtgcgacgt gacccagcaa gaccaggtgg acgcgctggt
     2161 gaacgaggcc gatgacgtgc tcagcggcac cgacatcctg ttcaacaacg ccggcctggg
     2221 cggccagaag gcgctggtgg agatgaccga cggcgaatgg ctcagggtca tcgacgtgtc
     2281 gctcaatggc accttccgca tgacgcgggc gatgatgaag aagatgatcc cgcgcggcaa
     2341 gggcgcgatc atcaacaact cgtcggtgct gggttggcgc gcccagaagg agcaggcgca
     2401 ctacgcggcg gccaaggccg gcgtcaacgc gctgacccgc tgcgccgcgc tcgaggcggc
     2461 cgaacacggc atccgcatca acgcggtgtc gccgagcatc gcgatgcacg actttctgaa
     2521 gaagaccgcg gacgaaggcc tgctggcccg cctggccgag cgcgaagcct tcggacgtgc
     2581 ggccgaagtg tgggagatgt cgaacatcgt cgtcttcctg gccagcgact atgcgagcta
     2641 cctggtcggc gagatcatcg cggccagcag ccagcacgcc tgagacggac gaggcaccga
     2701 tggccaccgt gatcagcagt gcgacccagc tcgccggcct cgtcggccag gagttgggtg
     2761 tgaccgattg gctcgaggtc acgcaagagc gcgtgaacca gttcgccgac gccaccggcg
     2821 accaccagtg gatccacgtc gacccggcgc gcgccgcggg cgggcccttc ggcggctgcg
     2881 tcgcgcacgg ctatctttcc ctcgccctgg cgggcagcta cttcctggag cgcatcgtca
     2941 a
//
LOCUS       DATMJB010000163         2837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_689247, whole genome shotgun sequence.
ACCESSION   DATMJB010000163 DATMJB010000000
VERSION     DATMJB010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689247"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VNU48_03625"
     CDS             complement(<1..143)
                     /locus_tag="VNU48_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliQ"
                     /protein_id="HWI10397.1"
                     /translation="MDSQQVFTAGQQGLYMLLMVSAPLLLAILVVGLAVSIFQAATQI
                     HEAT"
     gene            complement(151..903)
                     /gene="fliP"
                     /locus_tag="VNU48_03630"
     CDS             complement(151..903)
                     /gene="fliP"
                     /locus_tag="VNU48_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059128.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HWI10398.1"
                     /translation="MRRLLALAALTVLSAVPAAAVAQATGTATLPLVIGQGAGGTSYS
                     VPIQTLLFFTALSFLPAVLLLMTGFTRIVIVLSLLRQAMGTQAAPPNQVVIGLSLFLT
                     FFVMGPTIDKVYADAYQPYSENKIAFDEALKRGEAPMRQFMLKQTRQADVMLFAKLAK
                     IDPAVKTADVPFKVLVPAFVTSELKSAFQIGFLIFIPFLIIDMIVASVLMSLGMMMLS
                     PVLIALPFKLMLFVLADGWNLLLGSLAASFAT"
     gene            complement(926..1279)
                     /locus_tag="VNU48_03635"
     CDS             complement(926..1279)
                     /locus_tag="VNU48_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HWI10399.1"
                     /translation="MISNGFNSLLWFIAIIAMIPVALWLLKRTPMGNGGGNGVMRSIA
                     TLPLSANQRVVMVEVGQGEARRCLVLGVTAQSITALYSLGPQDEVPVAAPAAVPAFAQ
                     LLGRLRQDKGTVGEG"
     gene            complement(1287..1724)
                     /gene="fliN"
                     /locus_tag="VNU48_03640"
     CDS             complement(1287..1724)
                     /gene="fliN"
                     /locus_tag="VNU48_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HWI10400.1"
                     /translation="MSAENTAPADDADAMAAEWAAALAESKPETASEVHAAADQVAPA
                     AFQNFAPTGATSAGNDINMILDIPVQLTVELGRTRIPIKHILQLAQGSVVELEAMAGE
                     PMDVLVNGYLIAQGEVVVVNDKFGIRLTDIVTPSERMRRLSRG"
     gene            complement(1714..2718)
                     /gene="fliM"
                     /locus_tag="VNU48_03645"
     CDS             complement(1714..2718)
                     /gene="fliM"
                     /locus_tag="VNU48_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HWI10401.1"
                     /translation="MNQQILSQDEVDALLQGITGESQKLDEAPVDNGAVRNYDLASQE
                     RIVRGRMPTMEIINERFARNIRIGLFNFIRKSPEIAIGSIKVQKYSAFLREIVVPTNF
                     NIMSVRPLRGSGLIVCDPTLVFAVIDALFGGAGKYHTRIEGRDFSPTEQRIIGRLVDV
                     ITAEYKKAWTGIYPLELDYQRSEMQPQFANIATPSEIVVATSFTLEIGDTSGTIHFCI
                     PYATLEPIRDVLYSTIQGDAAEPDRRWVNLMKQQIQSAEVELIAELAQAPATVEQLLS
                     FKVGDFIELDLQQAIQAKVVGVPVFDCHYGTSNGKYALKVHQLLTNAEQGWLGESHVS
                     "
BASE COUNT          462 a          924 c          952 g          499 t
ORIGIN      
        1 gtcgcttcgt ggatctgcgt cgcggcctgg aagatgctga ccgcgagccc gacgaccagg
       61 atcgcgagca gcagcggcgc cgagaccatc agcagcatgt acaggccctg ttggccggcg
      121 gtgaaaacct gttgtgagtc catggcgtga tcaggtggcg aaacttgcgg cgagcgaacc
      181 gagcagcagg ttccagccgt cggcgagcac gaacagcatc agcttgaacg gcaacgcgat
      241 cagcacgggg gagagcatca tcatgccgag cgacatcaac acgctggcga cgatcatgtc
      301 gatgatcagg aacgggatga agatcagaaa gccgatctgg aacgcgctct tcagctcgct
      361 cgtcacgaag gccggcacca gcaccttgaa cggcacgtcg gcggtcttca cggccgggtc
      421 gatcttcgcg agcttggcga acagcatcac gtcggcctgg cgcgtctgct tcagcatgaa
      481 ctggcgcatc ggcgcctcgc cgcgcttgag cgcctcgtcg aaggcgatct tgttctccga
      541 gtacggctgg tacgcatcgg cgtagacctt gtcgatcgtc gggcccatca cgaagaaggt
      601 caggaacagg ctcaggccga tcacgacctg gttcggcggc gccgcctgcg tgcccatcgc
      661 ctggcgcagc aggctcagca cgatcacgat gcgcgtgaag ccggtcatca gcagcagcac
      721 cgcgggcagg aacgacagcg cggtgaagaa cagcagggtc tggatcggca ccgagtagct
      781 ggtgccgccg gccccctggc cgatcaccag cggcagggtc gcggtgcccg tggcttgcgc
      841 gacagcggcg gccggaacgg ccgacaacac cgtcaaggcc gcgagcgcga gcagtcggcg
      901 caggcgcgcc gaaagaacag ggcgctcagc cctcaccgac cgtccccttg tcctggcgca
      961 gccggcccag cagctgcgcg aacgccggca ccgcggccgg cgcggcgacc ggcacctcgt
     1021 cctgcggccc cagcgagtac agggccgtga tgctctgcgc ggtcacgccc agcaccaggc
     1081 agcggcgcgc ttcgccctgg ccgacctcga ccatgacgac gcgctggttc gccgacagcg
     1141 gcagcgtcgc gatgctgcgc atcacgccgt tgccgccgcc attgcccatc ggcgtgcgtt
     1201 tcagcagcca cagcgccacc ggaatcattg cgatgatcgc gatgaaccac agcagcgagt
     1261 tgaatccgtt cgagatcatt gcgggttcag ccccggctca ggcggcgcat gcgctcgctc
     1321 ggcgtgacga tgtccgtcag gcggatgccg aacttgtcgt tcacgaccac gacttcgccc
     1381 tgcgcgatca ggtagccgtt gacgagcacg tccatcggct cgccggccat cgcctcgagc
     1441 tcgaccaccg agccctgcgc gagctgcagg atgtgcttga tcgggatgcg cgtgcggccc
     1501 agttccacgg tgagctgcac cgggatgtcc aggatcatgt tgatgtcgtt gccggcactg
     1561 gtcgcgccgg tgggcgcgaa gttctggaac gcggccggcg cgacctggtc ggcggcggcg
     1621 tgcacctccg aggccgtttc gggtttcgat tcggcgagcg cggcggccca ttcggcggcc
     1681 atcgcgtctg cgtcatcggc gggagcggtg ttttcagctg acatgtgatt ctcccagcca
     1741 accctgttcg gcgttggtca gcagttggtg gactttcaat gcgtacttgc cgttggacgt
     1801 gccgtagtgg cagtcgaaca ccggcacccc gaccaccttg gcctggatcg cctgctgcag
     1861 gtcgagctcg atgaaatcgc cgaccttgaa cgacagcagc tgctcgaccg tggccggcgc
     1921 ctgcgccagc tcggcgatca gctcgacctc ggccgactgg atctgctgct tcatcaggtt
     1981 gacccagcgc cggtcgggct cggcggcgtc gccctggatc gtcgaataca gcacgtcgcg
     2041 gatcggctcg agcgtcgcgt acgggatgca gaagtggatc gtgccgctgg tgtcgccgat
     2101 ctccagcgtg aagctggtcg cgacgacgat ctcgctcggc gtggcgatgt tcgcgaactg
     2161 cggctgcatc tccgagcgct ggtagtcgag ctcgagcggg tagatgccgg tccaggcctt
     2221 cttgtattcg gccgtgatga cgtcgaccag gcgcccgatg atgcgttgct cggtgggcga
     2281 gaagtcgcgc ccctcgatgc gcgtgtggta cttgccggcg ccgccgaaca gcgcatcgat
     2341 caccgcgaac accagcgtcg ggtcgcacac gatcaggccc gagccgcgca gcggccgcac
     2401 gctcatgatg ttgaagttgg tcggcaccac gatctcgcgc aggaaggcgc tgtacttctg
     2461 caccttgatg ctgccaatcg cgatctccgg gctcttgcgg atgaagttga acaggccgat
     2521 gcggatgttg cgcgcgaacc gctcgttgat gatctccatc gtgggcatgc gcccgcgcac
     2581 gatgcgctcc tggctcgcca ggtcgtagtt gcgcaccgcg ccgttgtcga ccggcgcctc
     2641 gtcgagcttc tggctctcgc cggtgatgcc ctgcagcagc gcgtcgactt cgtcttgcga
     2701 aagaatctgt tgattcattg gatgatgaag ctggagaagt gcacgtggcg cacggggctc
     2761 ttgtctagcg gcgtcttcgc ggacttgtcc tcgtcggtcg aggccggctt ggcgtcctcg
     2821 ttctcgtcct cgagctc
//
LOCUS       DATMJB010000164        10188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_570847, whole genome shotgun sequence.
ACCESSION   DATMJB010000164 DATMJB010000000
VERSION     DATMJB010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570847"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="VNU48_03650"
     CDS             <1..539
                     /locus_tag="VNU48_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI10402.1"
                     /translation="ALASGIPFNGMFLYVLSAPVFLGDHLHLAPGQFFWFFLITIAGI
                     MGGAWFSGRLAGRIKPQHQIRHGFLIMLLVSIVNLALNLLFTPQVWWALIPLGIYAFG
                     WALMVPVVTLMVLDLVPERRGMASSFQACIGSLANGLVAGLIAPLVMHSTIWLATASL
                     LMMSIGVMAWIWVKPRVT"
     gene            616..1668
                     /locus_tag="VNU48_03655"
     CDS             616..1668
                     /locus_tag="VNU48_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HWI10403.1"
                     /translation="MPAAPLSLFRRRRVVALFALSAACATALAAEHAPTPAPAAASAA
                     PASATADVRGPRDPMDLLRERLARKLGAARAPDAANPNVLRVTSKASAPAAVAERDAD
                     PAALAPTPPRRAPHVHAAAAEPAHADHWDYAGNGGPQSWGRMKPEFSKCSTGTRQSPI
                     DIRDGIRVELDPVQFDYKPSAFRVVDNGHTVQVNVAAGNSIEVMGRRYELVQFHFHRP
                     SEERIDGKQFDMVVHLVHKDLEGRLAVVAVLLERGSAQAVVQSVWNNLPLERGDEVAA
                     RSPLNLNELLPTERRYFTYMGSLTTPPCSEGVLWMVMKQPVPVSAEQIGIFARLYPMN
                     ARPIQSAAGRLIKESN"
     gene            1700..2272
                     /locus_tag="VNU48_03660"
     CDS             1700..2272
                     /locus_tag="VNU48_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HWI10404.1"
                     /translation="MALFLIGLVLFLGIHSVAIVAPAWRDAQAARHERVWKGVYALLS
                     IAGFALLIYGYGVARQTPAVLWTPPAAMRHIALLLMLPVFPLLLAAYLPGRIKTATKH
                     PMLLAVKLWATAHLLANGTLVDVILFGAFLVWAVLDRIAVKRRAVPRAIPGAPPSALN
                     DAIALVGGLAIYAAFLAGVHRWMIGVSPLG"
     gene            2432..2683
                     /locus_tag="VNU48_03665"
     CDS             2432..2683
                     /locus_tag="VNU48_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HWI10405.1"
                     /translation="MNKDQVKGRVEQAKGSVKEATGKVVGNKKLEAEGKIDKAVGKTQ
                     ATYGDLKEDAKDVMKHQEEVRHDREDLRRDEDDIRRNQP"
     gene            complement(2688..4790)
                     /locus_tag="VNU48_03670"
     CDS             complement(2688..4790)
                     /locus_tag="VNU48_03670"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HWI10406.1"
                     /translation="MFADSSALPRIPDPFALLNAQQRSAVEHGLEGAAPHTRAGPMLV
                     IAGAGTGKTMTLAARVARLVLAGADPQRILLLTFSRRAAQEMQRRVGRMLHQALGFAS
                     TQGAPQLHWAGTFHGIGARLLREYAPQIGLAENFTIQDRADSEDVMGLARHELKLADA
                     KARFPLKATCASIYSRVLNSQQPLAEVLRAAYPWCETWEDELRQLFRAYVAEKHAQCV
                     LDYDDLLLYWEQMLAEPALAAHVSARFDHVLVDEYQDTNRLQAAILHALRPDGRGLTV
                     VGDDAQSIYSFRAAELRNILDFPDRFTPPARIVTLERNYRSTQPILEASNEVIALAPQ
                     RFGKRLWSDAASTQRPSLVTVQDESAQATWVADQVLRHREQGTSLKSQAVLFRSAQHS
                     AALELELTRRNIPFVKFGGLKFLEAAHVKDLLSVLRWAQNPRSRMAGLRVAQLVPGIG
                     TASAKRLLDAMGNAPDPVAALRAFSPPAAAAAEWPAWVEAFAALQRAAWPLALELATR
                     WYVPQLRRLHDDAALREGDLAQLGRLAAGFGSCQNFLAELTLDPPEATSDESGPPLRD
                     EDYLILSTIHSAKGQEWKAVFVLNAVDGCIPSDMATGNAIEIEEERRLLYVAMTRARH
                     HLHLLVPQRFYVTQQARHGDRHLYGSLTRFIPPALEPCFDIEPAHEKAGAADDGISPA
                     PLPGLAIDLTARVRSLWS"
     gene            4835..5731
                     /locus_tag="VNU48_03675"
     CDS             4835..5731
                     /locus_tag="VNU48_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWI10407.1"
                     /translation="MSVLVGTASWSSKTLVDSGRFYPPGVSSAEARLRYYASRFPLVE
                     VDSSYYAMPSAATAQLWAERTPDDFVMNVKAFRLFTGHQTSPTVLHKDIRSALPASIK
                     SSLYYRDLPAEIVAELWRRFIEALAPLRASGRLGLVHFQFAPWLLRDAAAHAHVRLCV
                     ERMAGHTLSVEFRQRSWFDGEHATASTLAFERELGVVHTVVDGPQGFANSVPAVWEAT
                     HPTYALLRLHGRNADTWKIKGARDSSERFNYDYNDAELAALAQQVKRLAARAATTHVV
                     FNNNMEDQGQRNAAALAQFLRG"
     gene            complement(5818..6741)
                     /locus_tag="VNU48_03680"
     CDS             complement(5818..6741)
                     /locus_tag="VNU48_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWI10408.1"
                     /translation="MDTLIRLIAEYGLLAVFVNILVQQLGAPVPGYPLLMVTGALAAR
                     GELDVLALLGIAVVACVIADSVWYAIGRRAGRRVLRALCRISLSPDGCVRQTESIYTR
                     FGPPSLLVAKFVPGFASVATALAGAMRIRRGAFLLFDGLGALLWVGIGLGLGWLFAPA
                     IEQVIGTLAQFGKWGLMLVALAVALFVAAKWWQRYRFNVQLRMARVSVDALGELLDRG
                     ERPLIVDVRSQIARDDGRIPGAIASLGDELAPELAAHPKEALVVVYCACPNDASAVLA
                     ARKLLERGFRNVRPLAGGIDAWTAAGRALEA"
     gene            6834..7268
                     /locus_tag="VNU48_03685"
     CDS             6834..7268
                     /locus_tag="VNU48_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWI10409.1"
                     /translation="MRILLPVDGSEGALEAVHHALRLVRDGLRASFVLANVQEPASLY
                     EVVVAHNAELIEQVSEAAAAHSLAPAQALLRAAGLEFETEIGHGDPGHLLVDIAERCE
                     CDLVIVGARGQDVSDSGALGPVATALLQHAAAPVMVVRRGEA"
     gene            7302..7958
                     /locus_tag="VNU48_03690"
     CDS             7302..7958
                     /locus_tag="VNU48_03690"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HWI10410.1"
                     /translation="MPDYRHQRRRMVAMQIARRGVRDPRVLAAMRTVPRECFVDAALA
                     EFAYDDAPLPIDAGQTISQPYVVAAMIEAADLGPRDTVLEVGAGSGYAAAVLGRIVAR
                     VCAIERHRVLVDAAAARLAALGYRNIELRLGDGSTGWPEAAPFDAILVAAAGPDVPSA
                     LKAQLALGGRLVMPVGGASWQRLLKITRSGDADFREQELCGVSFVPLVGAQGWPEPGP
                     "
     gene            complement(8088..8942)
                     /locus_tag="VNU48_03695"
     CDS             complement(8088..8942)
                     /locus_tag="VNU48_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10411.1"
                     /translation="MKRFLHPAIFVIAVVIVLEECLWDGLKRGMRKLSALPIVAAAEQ
                     RLRKLGPWASLAVLMLPATILLPCKLAAVWALSHHHPMLGLTVLLAAKLAGTSMAAYL
                     FDIVRDSARQLVWFDRCCSAVIHALDRTKTWLARQPTYRAAVTAVATARARTARLVRR
                     LATRSRLRRKLHAAKVLAYAIPVAALRATQEPSFTVSEQLLGVPSANDEDEPDVWARW
                     PDGSMCAVGIDLCRALRRPGRSSDYQLVHVIEYAADGIPSEWVIRTPPESPPVSFSRA
                     ARRGSRYG"
     gene            complement(9083..10036)
                     /locus_tag="VNU48_03700"
     CDS             complement(9083..10036)
                     /locus_tag="VNU48_03700"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI10412.1"
                     /translation="MKPGRLATLRQATGPFIPLAVLLSLLGVIGVVNPPFLSLGNLGV
                     LADESSVILLLATAQTLVILLGCIDLSMAALAGLASVLIALAVPTMGGLGVLGVLALS
                     TLAGAFQGFVHARAQLPSVVVTLAGLGMWSGIALTIAHATIPVDAGYSAVAWLDGTYF
                     GLPGSFAFAAGALVVLAAALRWLPLGRHVHAIGLSPKVAVLSGIRVDRVKVLAFAVAG
                     LFAGLAGMTMVARTSSGNPTIADSLLLPCIAAVVMGGTAMTGGVGGLGRTLIGALTVT
                     LLRAGITAAGVDPAFEPIAYGILLVLAVALTADRRRAAVTK"
BASE COUNT         1593 a         3562 c         3580 g         1453 t
ORIGIN      
        1 tggcgctggc cagcggcatc ccgttcaacg gcatgttcct gtacgtgctg tcggcaccgg
       61 tgttcctcgg cgaccacctg cacctcgcgc cgggccagtt tttctggttc ttcctgatca
      121 cgatcgccgg gatcatgggc ggcgcgtggt tctcggggcg cctggccggg cgcatcaaac
      181 cgcagcacca gatccgccac ggtttcctga tcatgctgct ggtgtcgatc gtcaacctgg
      241 cgctgaacct gctgttcacg ccgcaggtct ggtgggcgct gatcccgctg ggcatctacg
      301 cgttcggctg ggcgctgatg gtgccggtcg tcacgctgat ggtgctcgac ctcgtgcccg
      361 agcggcgcgg catggcctcg tcgttccagg cctgcatcgg cagcctcgcg aacggcctgg
      421 tcgcggggct gatcgcgccg ctggtgatgc actcgacgat ctggctcgcg accgcctcgc
      481 tgctgatgat gagcatcggc gtgatggcgt ggatctgggt caagccgcgc gtcacctgag
      541 cggcaatctc aagttcgcca cgcggccggt cgataagcag gcgagaggcg ccgttgtcgc
      601 gccggagatc cgaccatgcc cgccgccccg ctatcgctgt tccgtcgccg acgcgtcgtc
      661 gcgctgttcg cgctgagcgc cgcgtgcgct accgcgctgg ccgccgagca cgcgccgaca
      721 ccggcccctg cggcggcgtc ggcggcgccg gccagcgcaa cggccgacgt gcgcggaccg
      781 cgcgatccga tggacctgct gcgcgagcgc ctggcgcgca agctcggcgc cgcacgcgcg
      841 cccgatgccg cgaatccgaa cgtgctgcgc gtgacctcga aggcgagcgc gccggccgcg
      901 gtggccgaac gcgatgccga cccggcagcg ctggcgccga caccgccgag acgcgcgccg
      961 cacgtgcacg cggcggccgc cgagccggcc catgccgacc actgggacta cgccggcaat
     1021 ggcggcccgc agagctgggg tcgcatgaag cccgaattct cgaagtgctc gaccggcacg
     1081 cgccagagcc cgatcgacat ccgcgacggc atccgggtcg agctcgaccc ggtgcagttc
     1141 gactacaagc cgagcgcgtt tcgcgtcgtc gacaatggcc acacggtgca ggtcaacgtg
     1201 gccgcgggca actcgatcga ggtgatgggc cggcgctatg agctggtgca gttccacttt
     1261 caccggccgt ccgaagagcg catcgacggc aagcagttcg acatggtcgt gcacctggtg
     1321 cacaaggacc tcgaaggccg gctcgcggtc gtcgcggtgc tgctcgaacg cggcagcgcg
     1381 caggcggtcg tgcagagcgt gtggaacaac ctgccgctcg aaagaggcga cgaggtcgcg
     1441 gcgcgcagcc cgctgaacct gaacgaactg ctgcccaccg aacgccgcta cttcacctac
     1501 atgggttcgc tgacgacgcc gccctgcagc gaaggcgtgc tgtggatggt gatgaagcaa
     1561 ccggtgccgg tctcggccga gcagatcggc atcttcgcgc gcctgtaccc gatgaacgcg
     1621 cggccgatcc agtcggccgc cgggcggctg atcaaggagt cgaactagac tacgcgttcc
     1681 ttcatcgcgg gagcgggaga tggcgctgtt tctgatcggc ctggtgctgt tcctcggcat
     1741 ccactcggtg gcgatcgtcg cgccggcctg gcgcgacgcg caggccgcgc gccacgagcg
     1801 cgtgtggaag ggcgtgtatg cgctgctctc gatcgccggt ttcgcgctgc tgatctacgg
     1861 ctacggcgtc gcacgccaga caccggccgt gctctggacg ccacccgccg cgatgcgcca
     1921 catcgccttg ctgctgatgc tgccggtgtt tccgctgctg ctggcggcct acctgcccgg
     1981 gcgcatcaag accgcgacga aacacccgat gctgctggcc gtcaagctgt gggccaccgc
     2041 ccacctgctg gccaacggca cgctggtcga tgtgatcctg ttcggcgcct tcctggtctg
     2101 ggccgtgctc gaccgcatcg cggtgaagcg ccgtgccgtg ccgcgcgcga ttcccggcgc
     2161 gccgccatcg gcgctgaacg acgcgatcgc gctggtcggc ggcctggcga tctacgccgc
     2221 attcctggcc ggtgttcacc ggtggatgat cggggtctcc ccgctggggt gaggccatgt
     2281 aacgggcgtg cgacacgtcc tacaggcgat tcgtgcccag cccgacagac cccggttcgc
     2341 gcgacggcca cagtggatgc atcgatccag cgggcatgac tgatcgcccg atgcaggtcg
     2401 atgtcactct ctccattgca aaggaagcag catgaacaag gaccaggtga agggccgcgt
     2461 cgagcaggcc aagggcagcg tcaaggaagc caccggcaag gtcgtgggca acaagaaact
     2521 cgaggccgag ggcaagatcg acaaggccgt cggcaagacc caggcgacct atggcgatct
     2581 gaaggaagac gccaaggacg tgatgaagca ccaggaagag gtcaggcacg atcgggaaga
     2641 tctgcgccgc gacgaggacg acatccgccg caaccagccc tgagcgatca ggaccacagc
     2701 gacctgacgc gcgccgtcag gtcgatcgcg aggccgggca gcggcgcggg cgaaatcccg
     2761 tcgtcggctg cgccggcctt ttcgtgtgcc ggctcgatgt cgaaacaggg ctccaacgcc
     2821 ggcggaatga aacgcgtcag cgagccgtag agatgccggt cgccgtggcg cgcctgctgc
     2881 gtcacgtaga agcgctgcgg caccagcaga tgcaggtggt ggcgcgcgcg cgtcatcgcc
     2941 acgtagagca ggcggcgctc ttcctcgatc tcgatcgcgt tgccggtcgc catgtccgat
     3001 ggaatgcagc cgtcgacggc gttcagcacg aacaccgcct tccactcctg gcccttcgcg
     3061 gaatggatcg tcgacaggat caggtagtcc tcgtcgcgca gcggcgggcc cgattcgtcg
     3121 ctggtcgctt ccggcggatc gagcgtcagc tcggccagaa agttctggca cgacccgaag
     3181 ccggcggcca agcgcccgag ctgcgccagg tcgccctcgc gcagcgcggc gtcgtcgtgc
     3241 aggcgccgca gctgcggcac gtaccaccgg gtcgcgagct ccagcgcgag cggccacgcc
     3301 gcgcgctgca gcgccgcgaa agcctcgacc cacgccggcc attcggcggc ggcagcagca
     3361 ggcgggctga acgcgcgcaa tgccgcgacg gggtcgggcg cgttgcccat cgcatcgagc
     3421 agccgcttcg cgctggccgt gccgatgccc ggcacgagtt gcgcgacgcg caggccggcc
     3481 atccgactgc gcgggttctg ggcccagcgc aacaccgaca gcagatcctt cacatgcgcg
     3541 gcttccagaa acttcagccc gccgaacttg acgaacggga tgttgcgtcg cgtcagctcg
     3601 agttcgagcg cggcgctgtg ctgcgcgctg cggaacagca ccgcctggct cttcagtgac
     3661 gtgccctgtt cgcgatgccg cagcacctgg tcggcgaccc acgtggcctg cgcagattcg
     3721 tcctgcaccg tgaccagcga cggccgctgc gtcgacgccg catcgctcca cagccgcttg
     3781 ccgaagcgct gcggcgccaa cgcgatgacc tcgttcgagg cctcgaggat cggttgcgtc
     3841 gaacggtagt tgcgctccag cgtgacgatg cgcgccggcg gcgtgaagcg atccgggaag
     3901 tcgaggatgt tgcgcagttc ggcggcacgg aacgagtaga tcgattgcgc gtcgtcgccg
     3961 accacggtga ggccgcggcc gtcgggcctg agcgcatgca ggatcgcggc ctgcaagcgg
     4021 ttcgtgtcct ggtattcgtc gaccagcacg tgatcgaagc gcgccgacac atgcgctgcc
     4081 agcgccggct cggccagcat ctgctcccag tacagcagca ggtcgtcgta gtcgagcacg
     4141 cactgcgcgt gcttctcggc cacataggcg cggaacagct gcctcagctc gtcttcccac
     4201 gtctcgcacc aggggtaggc cgcgcgcagc acctcggcca ggggctgctg gctgttgagc
     4261 acgcgcgagt agatcgacgc acaggtggcc ttcagcggga agcgggcctt cgcgtccgcg
     4321 agcttcagct cgtggcgcgc cagacccatc acgtcctcgg agtcggcgcg gtcctggatc
     4381 gtgaagttct cggccaggcc gatctgcggc gcgtactcgc gcagcagccg cgcgccgatg
     4441 ccgtgaaagg tgccggccca atgcagctgc ggcgcgcctt gcgtggatgc gaagccgagc
     4501 gcctggtgca gcatgcggcc gacgcggcgc tgcatctctt gcgcggcgcg gcgcgagaag
     4561 gtcagcagca ggatgcgctg cgggtcggcg ccggccagca ccagccgcgc gacgcgcgcg
     4621 gccagcgtca tcgtcttgcc agtgccggcg ccggcgatca ccagcatcgg gccggcccgc
     4681 gtgtgcggcg cggcgccctc caacccgtgc tcgaccgcgc tgcgctgctg tgcgttgagc
     4741 agtgcgaacg ggtcggggat gcggggcagg gcagaagaat cggcaaacat cggcatactg
     4801 taattgcatc cagccgcagc ttcgaagaat caccatgtcc gttctcgtcg gcaccgcgtc
     4861 gtggtccagc aagaccctgg tcgacagcgg tcgcttctat ccgcccggcg tgagcagcgc
     4921 cgaggcgcgc ttgcgctact acgcatcgcg cttcccgctc gtcgaggtcg attcgagcta
     4981 ctacgcgatg cccagcgccg cgacggcgca gctctgggcc gagcgcacgc cggacgactt
     5041 cgtgatgaat gtcaaggcgt tccggctgtt caccggccac cagacttcac cgaccgtgct
     5101 gcacaaggac atccgttcgg ccttgcctgc ctcgatcaag tcaagcctgt actaccgcga
     5161 tctgccggcc gagatcgtcg ccgaactctg gcgccgcttc atcgaggcgc tcgcgccgct
     5221 gcgggcgtcg ggccggctcg ggctggtgca cttccagttc gcgccctggt tgctgcgcga
     5281 cgccgccgcc cacgcgcacg tgcggctgtg cgtcgagcgc atggccggcc acacgctcag
     5341 tgtcgagttc cgccagcgca gctggttcga cggcgagcat gcgaccgcgt cgacgctcgc
     5401 gttcgagcgc gaactcggcg tcgtgcacac cgtggtcgac ggcccgcaag gcttcgcgaa
     5461 cagcgtgccg gccgtgtggg aagccaccca tccgacgtac gcgctgctgc ggctgcacgg
     5521 ccgcaatgcc gacacctgga agatcaaggg cgcgcgcgat tcgtcggagc gtttcaacta
     5581 cgactacaac gacgccgaac tggcggcgct ggcgcagcag gtcaagcggc tcgcggcgcg
     5641 ggccgcgacc acgcacgtcg tcttcaacaa caacatggaa gaccagggcc agcgcaacgc
     5701 ggccgcgctg gcgcagttcc tgcgcggctg agcagcctca ggcgagcgcc ggggccgccc
     5761 caagccgcct gagttccccg cgggggacga cgccgaaggc gtccaggggt cgtcatccta
     5821 ggcctcgagc gcgcggccgg cggccgtcca cgcgtcgatg ccgccggcga gcgggcgcac
     5881 attgcgaaag ccccgctcga gcagcttgcg cgccgcgagc acggccgagg catcgttcgg
     5941 gcaggcgcag tagacgacga ccaacgcttc cttcgggtgg gcagccaact ccggcgcgag
     6001 ttcgtcaccg aggctcgcga tcgcgcccgg gatgcgcccg tcgtcgcgcg cgatctgcga
     6061 gcgcacgtcg acgatcagcg ggcgctcgcc gcggtcgagc agctcgccca gcgcatcgac
     6121 ggagacgcgc gccatgcgca gctgcacatt gaagcggtag cgctgccacc acttcgcggc
     6181 gacgaacagc gccaccgcca gcgcgaccag catcagcccc catttgccga actgcgccag
     6241 cgtgccgatg acctgctcga tcgccggcgc gaacagccag cccaggccca gcccgatgcc
     6301 gacccacagc agcgcgccga gcccgtcgaa cagcaggaac gcgccgcgcc ggatgcgcat
     6361 cgcgccggcc agcgcggtcg ccaccgacgc gaagcccggc acgaacttcg ccaccagcag
     6421 cgacggcggg ccgaagcgcg tgtagatcga ttcggtctgg cgcacgcagc cgtcgggcga
     6481 cagcgagatg cggcacaagg cgcgcagcac gcgccggccg gcgcgccggc cgatcgcgta
     6541 ccagacgctg tcggcgatca cgcaggccac gaccgcgatg ccgagcagcg cgagcacgtc
     6601 gagctcgccg cgcgccgcca gcgcgccggt gaccatcagc aaggggtagc ccggcaccgg
     6661 cgcgccgagc tgctggacga gaatgttgac gaacacggcg agcaggccgt actcggcgat
     6721 caggcggatc agggtgtcca aggcgttgtg cgtggtgagg gctgccagtg tcgcccggat
     6781 cggcgccgcc cgcaggacgt aagctcggcc attcgccgcc agaggagaga cgcatgagaa
     6841 ttctgttgcc cgtggacgga tcggaggggg cgctcgaggc cgttcaccac gcgctgcggc
     6901 tggtgcgcga cgggctgcgc gcgagcttcg tgctcgccaa cgtccaggag ccggcctcgc
     6961 tgtacgaggt cgtcgtggcc cacaacgccg agctgatcga gcaggtcagc gaagccgcgg
     7021 ccgcgcactc gctggcgccg gcgcaggcat tgctgcgcgc cgccggcctc gagttcgaga
     7081 ccgagatcgg ccacggcgac cccggccacc tgctggtcga catcgccgag cgctgcgagt
     7141 gcgatctggt gatcgtcggt gcgcgcggcc aggacgtgtc ggacagcggc gcgctcggcc
     7201 cggtggccac cgcgctgctg cagcacgcgg ccgcgccggt gatggtggtc aggcgcggcg
     7261 aggcctgaac cgtcggtggc gcgcgcgaga atcgcgtcgt catgcccgac taccgacacc
     7321 agcgccggcg gatggtggcg atgcagatcg cgcggcgcgg cgttcgcgac ccgcgcgtcc
     7381 tggccgcgat gcgcacggtg ccgcgcgagt gcttcgtgga cgccgcgctg gccgagttcg
     7441 cctacgacga cgccccgctg ccgatcgacg ccggccagac catctcgcag ccctatgtcg
     7501 tcgcggcgat gatcgaagcc gcggacctcg gtccgcgcga cacggtgctc gaggtcggcg
     7561 ccggctcggg ctatgcggcg gcggtgctcg ggcgcatcgt cgcccgggtc tgcgccatcg
     7621 agcggcaccg cgtgctggtc gacgccgccg cggctcgcct ggccgcgctc ggctaccgca
     7681 acatcgagct gcgcctcggc gatggcagca ccggctggcc cgaggcggca ccgttcgacg
     7741 cgatcctggt ggcggcggcc gggccggacg tgccgagcgc cctgaaggcg cagcttgcgc
     7801 tcggcgggcg cctggtcatg ccggtcggcg gcgcgtcgtg gcagcggctg ctgaagatca
     7861 cgcgttcggg cgacgccgac tttcgcgagc aagaactctg cggggtgtcg ttcgtgcccc
     7921 tggtcggcgc ccaggggtgg cccgagccgg gaccgtgagt ggggagggtc gagtgacgac
     7981 cctcagcgca ggaggcgctt cagcctcacg accgtcgccc gcaagtcaag cggcttggtc
     8041 catggctccc ggccggcgtc ataaaggtcg ctccctcgcc tcgctaacta accgtaccgt
     8101 gagcctcgac gcgccgcgcg actgaacgac acgggcggcg actccggcgg tgttcggatg
     8161 acccactcgg atgggatccc atctgcggcg tattcgatga cgtggaccag ctggtagtcg
     8221 ctcgacctgc ccggccgacg cagcgcccgg cacaggtcga tgccgaccgc gcacatcgac
     8281 ccatcgggcc atcgcgccca gacatcgggc tcgtcttcgt cattggcgga gggcactccc
     8341 agcaattgct ccgacacggt gaagctcggt tcctgtgtcg ccctcagcgc ggcaacgggg
     8401 atcgcgtagg ccagcacctt ggccgcgtgc agcttcctgc gcagacgcga acgcgtcgcg
     8461 aggcggcgca ccaggcgcgc cgtacgcgcg cgtgccgtcg cgaccgccgt gaccgccgcc
     8521 cgatacgtcg gctgtcgggc gagccaggtc ttggtgcgat cgagcgcgtg gatcacggct
     8581 gaacagcaac ggtcgaacca caccagctgc cgggcggagt cgcgcacgat gtcgaagagg
     8641 taggccgcca tcgaggtgcc ggcgagcttc gccgcgagga gcacggtcag ccccagcatg
     8701 gggtgatggt gcgaaagcgc ccagaccgct gccagtttgc atggaaggag gatggttgcc
     8761 ggcagcatga gcaccgccag cgaagcccat ggccccagct tgcgcaatcg ctgctcggcg
     8821 gccgccacga tcggtagcgc cgagagtttt cgcattccgc gcttcagccc gtcccagagg
     8881 cattcttcga gaacgatgac gacggcgatc acgaagattg cggggtgaag gaagcgcttc
     8941 atgcaatggc gtcgacggtg cggcaagggt gcggcgcgag catacccacc cgtctgcggt
     9001 ttgggcgggc gacggcatca agacgatgcg gcatgttccc gtgccccccg tcgttttgtc
     9061 tgggcgatga caggcgggcc ggtcatttcg ttaccgcggc ccggcgccgg tcggcggtca
     9121 gggccacggc caacaccagc aggatgccgt aggcgatggg ctcgaacgcg gggtcgacgc
     9181 cagccgccgt gatgccggcg cgaagcaggg tgacggtcaa cgcgccgatc agggtgcgcc
     9241 cgagcccgcc caccccgccc gtcatggccg tgccgcccat caccacggcg gcgatgcacg
     9301 gcagcagcag gctgtctgcg atcgtcgggt tgccgctgga cgtgcgcgcg accatcgtca
     9361 tgcccgccag cccggcgaac aacccggcca ccgcgaaggc gagcaccttg acccgatcga
     9421 cacgaatgcc gctcaacacc gccaccttcg gactcagacc gatcgcgtgg acatggcggc
     9481 ccagcgggag ccagcgcagg gccgccgcca gtaccacgag tgccccggcc gcaaaggcga
     9541 acgaaccggg cagcccgaag tacgtgccgt cgagccaggc caccgccgag tagcctgcgt
     9601 cgacgggaat ggtcgcgtgg gcgatcgtca gcgcgatgcc cgaccacatg cccagcccgg
     9661 cgagcgtcac caccacggaa ggcaactggg cgcgggcatg aacgaagccc tggaacgcgc
     9721 cggcgagggt gctcagcgcc agcacgccca gcaccccgag cccccccatc gtcggcactg
     9781 ccagcgcgat cagcaccgac gccagcccgg ccagcgcggc catcgacagg tcgatgcagc
     9841 cgagcaggat caccagcgtc tgtgcggtcg ccaagagcag gatcacgctc gattcgtcgg
     9901 ccagcacgcc gaggttgccg agcgacagga agggaggatt gaccaccccg atgacgccca
     9961 ggagcgacag cagcaccgcc agcggaatga acgggccggt ggcctgtcgg agggtcgcaa
    10021 ggcggccggg tttcacgtca gttgctccac cgcgcgcgct gcgcgacccg cgacgcatga
    10081 agcgctgcgg tggcttggga tgtctgcttg cggcccgttg cggtcgttcg caagcgagcg
    10141 tgcgcccttt gacggtcggc gcgagccgag agagtcgggt ggacaact
//
LOCUS       DATMJB010000165         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_882849, whole genome shotgun sequence.
ACCESSION   DATMJB010000165 DATMJB010000000
VERSION     DATMJB010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882849"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VNU48_03705"
     CDS             <1..1080
                     /locus_tag="VNU48_03705"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HWI10413.1"
                     /translation="GVSGYKGFFYHFLDMKTGLRAPKSELSTVDTALLIGGMLHAQAF
                     FDREHADEVAIRDAVDQIYWRIDWKWAQVRGQLISMGWSPEQQFIPHDWNGYNEAMLV
                     VLLALGSPTHPVDEGAWSAWTESYRGTWGRFMGYEHLSFAPLFGHQYSHVWIDFRGLQ
                     DATMRQRGIDYFENTRRAVYAQRAYAIANPNGWFEYGANVWGFTACDGPGKMREVDRS
                     GRRRSFLDYSARGAGRAHTVDDGTIAPTAAVSSLPFAPEIVIPAVEEMHARYGFYIYS
                     KYGYLDSFNRSFTATDVPLADGRVDHGFGWVAKDYLGIDQGPILAMICNYRNELVWDD
                     MRKCVPIRRGLERAGFTGGWLTKTG"
     gene            1109..>2323
                     /locus_tag="VNU48_03710"
     CDS             1109..>2323
                     /locus_tag="VNU48_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWI10414.1"
                     /translation="MTMWRRGALKGLGALALGGAGALAGCGRRDDPRGPLNFWAMGRE
                     AEVVAQLLPEFGARHPGVQVRVQQLPWTAAHEKLLTAYAGDSLPDLCQLGNTWLPEFV
                     ALDALEPLDARVAASSVRRDDYFAGILDTNLMPTPQGTQLFGLPWYVDTRLLFYRSDL
                     LRAAGFERAPQSWAEWLDAMRAVTRRGAGRGFGALLPLNEPDPLFAFALQQGALLADD
                     DTRGAFARPAFRRALDFYAGIFHEGLAPLATNTQISNVWDEFARGLFTFYVSGPWNIG
                     EFRRRLPRDRQNDWATAPLPGPDGPGVSTAGGSSLVMFKRSPRQDAAWKLIEFLSEPA
                     TMQRFHALTGDLPPRRSAWRAPALANDVPSQAFAQQLERVRPAPKIPEWERIFQEMQL
                     MAERVVQRTQAAD"
BASE COUNT          335 a          828 c          815 g          345 t
ORIGIN      
        1 ggcgtgagcg gctacaaggg attcttctat cacttcctcg atatgaagac cgggctgcgc
       61 gccccgaaga gcgagctctc gacggtcgac accgcgctgc tgatcggcgg catgctgcac
      121 gcgcaggcct tcttcgaccg cgagcacgcc gacgaggtcg cgatccgcga cgccgtcgac
      181 cagatctact ggcgcatcga ctggaagtgg gcgcaggtgc gcggccagtt gatcagcatg
      241 ggctggagcc ccgagcagca gttcatcccg cacgactgga acggctacaa cgaggcgatg
      301 ctggtcgtgc tgctcgcgct gggctcgccg acgcacccgg tcgacgaagg cgcgtggagc
      361 gcctggaccg aaagctaccg cggcacctgg ggtcgcttca tgggctacga gcacctgagc
      421 ttcgcgccgc tgttcgggca ccagtactcg cacgtctgga tcgacttccg cggcctgcag
      481 gacgcgacga tgcgccagcg cggcatcgac tacttcgaga acacgcgccg cgccgtctat
      541 gcgcagcgcg cctacgcgat cgcgaacccg aacggctggt tcgagtacgg cgccaacgtc
      601 tggggcttca ccgcctgcga cggcccgggc aagatgcgcg aggtcgaccg cagcgggcgc
      661 cggcgcagct ttctcgacta ctcggcgcgc ggcgccgggc gtgcgcacac cgtcgacgac
      721 ggcacgatcg cgccgaccgc cgcggtgtcg tcgctgccgt tcgcgcccga gatcgtgatc
      781 ccggcggtcg aggagatgca tgcgcgctac ggcttctaca tctactcgaa atacggctac
      841 ctcgactcgt tcaaccgcag cttcaccgcg accgacgtcc cgctcgccga tggccgcgtc
      901 gaccacggct tcggctgggt cgcgaaggac tacctcggca tcgaccaggg cccgatcctg
      961 gcgatgatct gcaactaccg caacgagctc gtctgggacg acatgcgcaa atgcgtgccg
     1021 atccggcgcg gcctcgagcg cgccggcttc accggcggct ggctcacgaa gaccggctga
     1081 tgcgcgcgcg gcgcggtctg gtgcgctcat gacgatgtgg cgacgcggcg cgctgaaagg
     1141 actgggcgcg ctcgcgctgg gcggcgcggg tgcgcttgcc ggctgcggcc ggcgcgacga
     1201 tccgcgcggc ccgttgaact tctgggccat gggccgcgag gccgaggtcg tcgcccaatt
     1261 gctgcccgag ttcggcgcgc gccaccccgg cgtgcaggtg cgcgtgcagc aactgccctg
     1321 gaccgcggcg cacgagaagc tgctcaccgc ctacgccggc gattcgctgc ccgacctgtg
     1381 ccagctcggc aacacctggc tgcccgagtt cgtcgcgctc gatgcgctgg agccgctcga
     1441 tgcgcgggtc gccgcatcga gcgtgcggcg cgacgactac ttcgccggca tcctcgacac
     1501 caacctgatg cccacgccgc agggcacgca gctgttcggg ctgccctggt acgtggacac
     1561 gcggctgctg ttctatcgca gcgacctgct gcgcgcggcc ggcttcgagc gtgcgccgca
     1621 atcgtgggcc gagtggctgg acgcgatgcg tgcggtgacg cggcgcggtg ccgggcgcgg
     1681 cttcggcgcg ctgctgccgt tgaacgaacc cgacccgctg ttcgcgttcg cgctgcagca
     1741 gggcgcgctg ctcgccgacg acgacacgcg cggcgcgttc gcgcgccccg cgttccggcg
     1801 cgcgctcgac ttctatgccg gcatcttcca cgagggcctc gcgccgctgg cgaccaacac
     1861 gcagatctcg aacgtctggg acgagttcgc gcgcggcctg ttcaccttct acgtcagcgg
     1921 gccgtggaac atcggcgaat tccggcggcg cctgccgcgc gatcgccaga acgactgggc
     1981 gaccgcgcca ctgcccgggc ccgacgggcc gggcgtctcg accgcgggcg ggtcgagcct
     2041 ggtgatgttc aagcgctcgc cgcgccagga cgcggcctgg aagctgatcg agttcctcag
     2101 cgagcctgcg acgatgcagc gcttccatgc gctgaccggc gacctgccgc cgcggcgcag
     2161 cgcgtggcgc gcaccggcgc tggcgaacga cgtgccgtca caggccttcg cgcagcagct
     2221 cgagcgcgtg cgcccggcgc cgaagattcc cgaatgggag cgcatcttcc aggagatgca
     2281 gctgatggcc gagcgcgtcg tgcagcgaac gcaggccgcg gac
//
LOCUS       DATMJB010000166        11600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_393107, whole genome shotgun sequence.
ACCESSION   DATMJB010000166 DATMJB010000000
VERSION     DATMJB010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11600
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393107"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VNU48_03715"
     CDS             complement(<1..933)
                     /locus_tag="VNU48_03715"
                     /inference="COORDINATES: protein motif:HMM:NF039286.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HWI10415.1"
                     /translation="MPLQHVPMCQRAPHTGFAKGWYRVADSAALQADTLMPVYWLDQQ
                     LIVYRTRAGAAQVSDAYCPHLGAHLASHDGRIENGEIVCPFHKWRWDGESGRCAGIPY
                     ASTLPPGTVRLTLHPTREVDGQVLVWHDPQGGEPGFEPFASPAFQRDRWVKYDQRSYV
                     TTCPFPDILENLFDGAHIVQLHHAARMPQLTNMRDTPHGMYLEYAIDAQAEDQPIEQI
                     AMNLSGISLLNQHYVARGWEALFFIALTPLDGERTEQSMSLYLKDLGSQEAHDALGKP
                     FVERFVYEVEQDLKVLDFKKHVLQPKLCRGDGPIY"
     gene            complement(939..1910)
                     /locus_tag="VNU48_03720"
     CDS             complement(939..1910)
                     /locus_tag="VNU48_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI10416.1"
                     /translation="MAATTSVEFHGQVAIVTGAGRGLGRAYAQLLARRGCKVVVNDLA
                     PALSGSPAQAVVDEIRAGGGTAQADLHDVAARPEAIVRSALDAFGRIDIVINNAGVTG
                     GGRIDEMPAADFDRLLDVNLRGAVGVLRAAWPHFVKQGYGRVVNTSSGSVLGLPGCFA
                     YQTSKAALIGLTRALALDGAPHGIKVNAINPIAYTRMTADIPDPAFRNFLETHFQPAL
                     AAPFVVALVCRDLPCSGELFSVGGGIAARVTLGYVPGRVLGADATPEDWLAQFDGVLA
                     TEGLRVAPHAMDEVAYRAQQIGAVLANANGAATDWSRPVHPPSGDPH"
     gene            complement(1914..2738)
                     /locus_tag="VNU48_03725"
     CDS             complement(1914..2738)
                     /locus_tag="VNU48_03725"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWI10417.1"
                     /translation="MFAVSDELAPRIKALGLERNVKEMAEFGCTVIEDAASPEFFERL
                     RSAIKRCCAQDKGQYFNITEKGESADLLLDRDPVFAEAVLNEKLLAMMEYMCGRNPLL
                     SQLTGSVRYQGAKAMALHCDQDWMPAPLPEHNAMMTGCWYCDDMTGESGATKVIPATH
                     LLRRQPSPEEVAAEKGAVPLKAPKYSIGLWDGRLWHSNYGRTLPGERVVLHATYCRLA
                     YRPLEDYGNLGKDFIDQYGPQMESLLGRNLWYGNRAIGNGGVDMSKYMTTWEAARR"
     gene            complement(2871..3470)
                     /locus_tag="VNU48_03730"
     CDS             complement(2871..3470)
                     /locus_tag="VNU48_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI10418.1"
                     /translation="MTAHATVDARAAQKSDTRARLLTAARRLFGQLGPALATVDQIAR
                     EAGTSRANFYLHFGGKDDVLHALRREMWRMALDFYEGFAELPDTSQETLHHWLQGVAQ
                     AWAQDGGLTRIVLGATPREVEQEYQEHVTQYIDALTRDAGKWRGFSKAQARQRAYLLI
                     VQLERCMNDQDHIDLPVERTSLLRTLAAVWAATLASPHR"
     gene            3584..4486
                     /locus_tag="VNU48_03735"
     CDS             3584..4486
                     /locus_tag="VNU48_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006481531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI10419.1"
                     /translation="MIDVKALGYIVAESTEPARWHDFGTQVLGAMAFDEADGGVRLKI
                     DERPYRILVQKGQADRYLASGWEVAHEQSFDKAFAELEAAGAHPRRATPAEKLSRRVS
                     GLLFFDDPSGNRHELFWGLVSDFARFHSPQDVPGYVTGDLGLGHTVLPAPKFDATWAF
                     WRDVMGFGLSDIYKHRAGPGAPVQRLHFTHCNPRQHSLALFEADSPFGCIHIMLEVDS
                     MTEVGRAMDRRAAHGAKLMASLGQHVNDNMTSFYMLTPSNFALEYGYGGLVVDWDKHA
                     VFEATTISQWGHDWSLGLATGDTK"
     gene            4495..5370
                     /locus_tag="VNU48_03740"
     CDS             4495..5370
                     /locus_tag="VNU48_03740"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWI10420.1"
                     /translation="MNKEISAADAVAQIRSGMTVGIGGWGPRRKPMALVRELLRSDVN
                     DLTVVAYGGPEVGMLCAAGKVKKLVYGFVTMDAIPLEPHFRKAREAGAIEVMELDEGM
                     FQWGLRAAGMRMSFLPTRCGLATDVLARNPQLKTVQSPYGDAEVYIAMPALKLDVALL
                     HVNEADRLGNTLTRSADPMFDDLFARAAERCFVSTETLVDRLSLDEHSARFNQFERYL
                     VTGVVHAACGAHPTYNGPDYGWDLEHLKRYNASASEEGGWQAYVDEFVAIGEAAYLAK
                     VGGAERIATLQRPVF"
     gene            5383..6183
                     /locus_tag="VNU48_03745"
     CDS             5383..6183
                     /locus_tag="VNU48_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacid CoA transferase"
                     /protein_id="HWI10421.1"
                     /translation="MTQNIDITLAELMAVACSEAWRGDGELLASGIGILPRLGASLAK
                     LTHSPELLMTDSETFLVEEPIPLGPRGDYVPKYSGYLSFSRVFDCVWGGRRHAMIGPT
                     QIDRWGQTNLSSVGDYAKPKTAILGVRGLPGNSINHPNSLFVPNHGPRVFVAGEVDMV
                     SGVGYNPARWAAGMKQDYVDLRLIVTDLCVMDFGGPDHAIRVTSLHPGVSFEQVQTAT
                     GFPLLKAPGLAVTPLPSDAQLAVIRRLDPHNLRATVIKNNPPAARHAQ"
     gene            6211..7110
                     /locus_tag="VNU48_03750"
     CDS             6211..7110
                     /locus_tag="VNU48_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWI10422.1"
                     /translation="MNQGLVDPKQVDIRYETDTPVRYAVEGPVAWITMDRTAFNNAQN
                     SQMTYALDDAFRRAVDDDAVKVIVLAGAGKHFSAGHDIGTPGRDVHKSWERKHLWYDH
                     TNKPGAEFLYTREQEVYLGMCRRWREAPKPTIAMVQGACIAGGLMLAWVCDLIVASDD
                     AFFQDPVVRMGIPGVEYFAHAFELPPRIAKEFLFMGQRMSAQRAEQFGMVNRVVPRSE
                     LEKTTREMAVSISEMPRLGLSLTKQAINHVEDLRGKRTAMDAVFHMHHFAHAQNDLVS
                     GNSLAGLDGKAMADANKKQAGKA"
     gene            7123..7875
                     /locus_tag="VNU48_03755"
     CDS             7123..7875
                     /locus_tag="VNU48_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HWI10423.1"
                     /translation="MSTPYTVEIDAGIAELVINQPPVNALDSAGWHALAARIDELGRD
                     PAVRVIVIRAEGRGFCAGVDIKELAAHPERIVAVNAGNYETFRAVHRNPVPVIAAVHG
                     FVLGGGIGITGAADIVVASECARFGVPEVDRGAMGGAAHLQRLFPVQKVRAMYFTGDM
                     IDAAEAYRLGAVEKLVPREQLRETALAIARKIAAKSPAMVRLAKESLNGIEDGDLEAK
                     YRWEQGFTLQAYMSPDSNETRQAFVTKREARF"
     gene            7895..9058
                     /locus_tag="VNU48_03760"
     CDS             7895..9058
                     /locus_tag="VNU48_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011355041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI10424.1"
                     /translation="MQLEYTDQQRAFRLEVRTWLQARVPRQPLQSFDTEQGFEQHRQW
                     EATLNSGRFAMVTWPKELGGRGCDLIEWLIFEEEYWRAGAPLRVNQNGIFLLGPTLMD
                     YGTPEQKARFLPRMAAGQDVWAQGWSEPNAGSDMAAIRCKATRNNGHYILNGQKIWST
                     RAVWADWLFGLFRSDPDSQRHHGLTFLLVPLKTPGITVRPIRQLNGLPGFAEIFFDDV
                     KVPVENRLHAEGEGWHVAMATAGFERGLMLRSPARFQSTAAALVGLYNKHRESADRDP
                     AVREAVLQAWMQAEAYALATYGTASRLMKGGAIGAESSTNKIFWSTLDQRMHETAMHI
                     LGARAELLPHAPAAGDVGNWLDGFLFAQAGPIYAGTNEIQRNIIAERMLGMPR"
     gene            9071..10138
                     /locus_tag="VNU48_03765"
     CDS             9071..10138
                     /locus_tag="VNU48_03765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI10425.1"
                     /translation="MDFTFTNDQSLFRDAIRSYLMVEATPELLRSVWETPSGRSDPAF
                     EKFAEQGLTGLSIPEAEGGLGCNELDWVLMAQELGYYGVPDSLLSTACLAVGLLTALP
                     GDAPVRKAWLPRILAGDARIAVGHPISALVADAHVADLLLLHHAGEVHAVPRSEVDLV
                     LNPSVDPARRLHTVAWKPSGRTRIADADAGRVLWDDMFERGALVTAAQSIGLTLRMMD
                     LAVDYSVQRKQFGKPIGSFQAVKHLMADVAVAVEFAKPVVYRAAHALAHRLPHRAVHV
                     SHAKLAADEAAWLAARNSIQVHGAMGYTWEVDLQIFMKRAWSLGASWGDRGLHKTRVS
                     DFVLADGAPLGPGNTFISTNH"
     gene            10147..11298
                     /locus_tag="VNU48_03770"
     CDS             10147..11298
                     /locus_tag="VNU48_03770"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWI10426.1"
                     /translation="MAEAYIVDALRTPTGKRGGALAPVHAADLGAFVLKTLMERNAVP
                     PGDVDDVIFGCVDAVGPNAGVISRTSWLAAGLPDHVPGTVIDRACGSSQQAVHFADQA
                     VMSGTQDVVVAGGVQNMSQVPIAYAMVAAQPLGFTDPFSGSKGWKERFGDKPVNQFYA
                     AQIIADKWGCSRDDMEAFAAESHTRALRAQAEGRFAREIVALNGLSHDETARAPNREK
                     MATLQPVDAKYPSITAAVSSSTCDAAAALLIVSEAALKRYGLKPRARIHHLSVLAADP
                     IWHLTAPIPATAAALKKAGMKIDDIDLVEINEAFASVVMAWLKETGYAHARTNVNGGA
                     LALGHPLGASGARLMTTLLHELERTGGRYGLQTMCEGGGQANVTIIERL"
     gene            11327..>11600
                     /locus_tag="VNU48_03775"
     CDS             11327..>11600
                     /locus_tag="VNU48_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI10427.1"
                     /translation="MGICDKRSVIVTGAGGGLGRAHALALAQAGASVLVNDIRREAAE
                     AVVVEIVAAGGKGIANSDDIASIAGAQRIIDAAVADFGDVQAVVNNA"
BASE COUNT         1879 a         3910 c         3964 g         1847 t
ORIGIN      
        1 gtagatcggc ccgtcgccac ggcacagctt gggctgcagc acgtgcttct tgaagtccag
       61 caccttcagg tcctgttcga cctcgtagac gaagcgctcg acgaagggct tgcccaatgc
      121 gtcatgcgct tcctgtgagc ccagatcctt caggtacagg ctcatgctct gctcggtgcg
      181 ctcgccgtcc agcggcgtca gtgcgatgaa gaacagggct tcccaaccgc gcgccacgta
      241 gtgctggttc agcagcgaga tgccgctcag gttcatcgcg atctgctcga tcggctggtc
      301 ctcggcctgc gcgtcgatcg cgtactcgag gtacatgccg tgcggcgtgt cgcgcatgtt
      361 ggtgagctgc ggcatcctgg cggcatggtg cagctgcacg atgtgcgcgc cgtcgaacag
      421 gttctcgagg atgtcgggga acgggcaggt ggtgacgtag ctgcgctggt cgtacttgac
      481 ccagcggtcg cgctggaagg ccgggctcgc gaacggctcg aagcccggtt cgccgccttg
      541 cgggtcgtgc cagacgagga cctggccgtc gacctcgcgc gtcgggtgca gcgtcagtcg
      601 aacggtgccc ggcggcaatg tcgaggcata cggaatgccg gcgcagcgcc cgctttcgcc
      661 gtcccagcgc cacttgtgga aggggcagac gatctcgccg ttctcgatgc ggccgtcatg
      721 cgatgcgagg tgggcgccca ggtgcgggca ataggcatcg ctcacttgcg ccgcgcccgc
      781 ccgggttcgg tagacgatca gttgctggtc gagccagtac accggcatca aggtgtcggc
      841 ttgcagcgcc gcgctgtcgg caacgcggta ccagcccttc gcgaaaccgg tgtgcggcgc
      901 gcgctggcac atcggaacgt gttgcagcgg cacggagctc aatgcgggtc tccagaaggt
      961 gggtgaacag ggcggctcca gtcggtcgcc gcgccgttgg cattggccag gaccgcgccg
     1021 atctgctgcg cgcggtaggc cacctcgtcc atcgcgtgcg gggccacgcg cagcccttcg
     1081 gtggccagca cgccgtcgaa ttgcgccagc cagtcttcgg gcgtggcgtc ggcgcccagc
     1141 acgcggccgg gcacgtagcc gagcgtcacg cgggcggcga tgccacctcc gaccgagaac
     1201 aactcgccac tgcagggcag gtcgcggcac accagggcga cgacgaaggg cgcggcgagt
     1261 gccggctgga aatgcgtttc gagaaagttg cggaaggccg ggtccggaat gtcggccgtc
     1321 atgcgcgtgt aggcaatcgg gttgatcgcg ttgaccttga tgccgtgcgg cgcgccgtcg
     1381 agtgcgagcg cgcgtgtcag gccgatcagc gcggccttgc tggtctggta ggcgaagcag
     1441 ccgggcaggc cgagcaccga gcctgaggac gtattgacga cgcgcccgta gccctgcttc
     1501 acgaagtgcg gccaggccgc gcgcagcacg cccacggcgc cgcgcaggtt cacgtcgagc
     1561 aggcgatcga agtcggcagc cggcatctcg tcgatccggc cgccgccggt gacgccggcg
     1621 ttgttgatga cgatgtcgat gcggccgaag gcgtcgagcg cgctgcgcac gatcgcctcc
     1681 ggccgcgcgg cgacatcgtg aaggtcggcc tgcgcggttc cgccgccggc ccgaatctcg
     1741 tcgaccacgg cttgtgccgg gctgccgctc agcgccggcg ccagatcgtt gacgacgacc
     1801 ttgcagccgc gccgtgcgag cagctgcgcg taggcccgcc ccagcccgcg gccggcgccc
     1861 gtgacgatgg ccacctggcc gtggaattcg acgcttgtcg tcgctgccat ctgtcagcgg
     1921 cgcgccgctt cccaggtcgt catgtacttg ctcatgtcca caccaccgtt gccgatggcg
     1981 cgattgccgt accagaggtt gcgcccgagc agcgactcca tctgcggacc gtactggtca
     2041 atgaagtcct tgcccaggtt gccgtagtct tccagcggcc ggtaggcgag gcggcagtag
     2101 gtggcatgca gcacgacgcg ctcgccgggc agcgtgcggc cgtagttgct gtgccagagc
     2161 cgcccgtccc acaggccgat cgagtacttg ggcgccttca gtggcacggc ccccttttcg
     2221 gccgcgactt cctccggcga gggctggcgg cgcagcaggt gcgttgcggg gatcaccttg
     2281 gtggcgcccg actcgcctgt catgtcgtcg cagtaccagc agccggtcat catcgcgttg
     2341 tgttcgggca gcggcgccgg catccagtcc tggtcgcagt gcagcgccat cgccttggca
     2401 ccctggtagc gcacgctgcc ggtgagctgg ctgagcaacg ggttgcggcc gcacatgtac
     2461 tccatcatcg cgagcagctt ctcgttgagc accgcttcgg cgaacaccgg gtcgcggtca
     2521 agcagcagat cggccgattc acccttctcg gtgatgttga agtattggcc cttgtcctgc
     2581 gcgcagcagc gcttgatggc gctgcgcaga cgctcgaaga actcgggcga ggctgcatcc
     2641 tcgatcacgg tgcagccgaa ctcggccatt tccttcacgt tgcgctccag gcccagggcc
     2701 ttgatgcgtg gcgccagttc gtcggagacg gcgaacatcg ggagggactc gtggggtggc
     2761 aatttgctca tctcggctcc gttcgtgtgg tgccgacagt ctggaagtcg cgacctcgac
     2821 ggccatcgtc cgatcagact agacgcgtgt ccagtgagcg cgctgacggc tcagcggtgc
     2881 ggtgatgcga gcgtcgccgc ccagacggcg gccagcgtgc gcaggagcga tgtgcgctcg
     2941 accggcaggt cgatgtggtc ctggtcgttc atgcagcgct ccagctgcac gatcagcagg
     3001 taggcgcgct ggcgggcctg ggctttcgaa aagccgcgcc atttgccggc atcgcgggtc
     3061 agcgcatcga tgtactgcgt cacgtgctcc tggtactcct gctcgacctc gcgaggcgtg
     3121 gcgcccagca cgatgcgggt cagcccgccg tcctgcgccc atgcctgggc gaccccttgc
     3181 agccagtggt gcagcgtctc ttgcgacgta tcgggcagtt cggcgaaacc ctcatagaaa
     3241 tccagtgcca tgcgccacat ctcgcgccgc agcgcgtgca gcacgtcgtc cttgccgccg
     3301 aaatgcaagt agaagttggc acgcgaggtg cccgcctcgc gcgcgatctg atcgacggtg
     3361 gccagggccg gaccgagctg gccgaacagg cgccgggctg cggtcagcag tcgtgcgcgc
     3421 gtgtcggact tctgcgcggc gcgtgcgtcg acggtggcat gcgcagtcat gtcgccatga
     3481 taggccgcgc acccgttctg ccttagtccc attggacgat gctgtgcacg ggctggtacg
     3541 gagaatccat tcgcactgaa acgcactccg ggagcgacaa ccgatgatcg acgtcaaggc
     3601 cctggggtac atcgtggccg agtccaccga gccggcgcgc tggcacgact tcggcaccca
     3661 agtgctgggc gcgatggcgt tcgacgaagc cgacggtggt gtgcgcctga agatcgacga
     3721 gcgcccttat cgcatcctgg tgcaaaaggg ccaagccgat cgctacctgg cctcgggctg
     3781 ggaggtggcg cacgagcagt cgttcgacaa ggcctttgcc gagctcgaag cggcgggtgc
     3841 ccacccgcgc cgcgcgaccc cggcggaaaa gctctcgcgc cgcgtcagcg gcctgctgtt
     3901 cttcgacgac ccgtccggca atcgacacga gctgttctgg ggtttggtgt ccgacttcgc
     3961 gcgcttccac tccccccaag acgtgcccgg ctacgtgacc ggcgacctgg gcctggggca
     4021 cacggtgctg ccggcgccga agttcgacgc cacctgggcg ttctggcgcg acgtgatggg
     4081 cttcggcctg tcggacatct acaagcaccg cgccggcccc ggcgcgccgg tgcagcgcct
     4141 gcacttcacg cactgcaacc cgcgccagca cagcctggcg ctgttcgagg cggacagccc
     4201 gttcggctgc atccacatca tgctcgaggt cgattcgatg accgaggtcg gccgcgcgat
     4261 ggaccgccgc gcggcgcacg gcgccaagct gatggccagc ctcggccagc acgtgaacga
     4321 caacatgacc tcgttctaca tgctcacgcc ttccaacttc gcgctcgagt acggctacgg
     4381 cggcctcgtc gtcgactggg acaagcacgc ggtgttcgag gccaccacca tcagccaatg
     4441 ggggcacgac tggagcctcg gcctggccac cggggacacg aagtgaaggc accgatgaac
     4501 aaggaaattt cggccgccga tgcggttgcg cagatccgca gcggcatgac cgtcggcatc
     4561 ggcggctggg gcccgcggcg caagccgatg gcactggtgc gcgagctgct gcgctccgat
     4621 gtgaacgacc tcaccgtggt ggcctacggc ggccccgagg tcggcatgct gtgcgcggcc
     4681 ggcaaggtga agaagctggt ctacggcttc gtcacgatgg acgcgattcc gctcgagccg
     4741 cacttccgca aggcgcgcga agccggcgcc attgaggtga tggagctcga cgaaggcatg
     4801 ttccagtggg gcttgcgcgc ggccggcatg cggatgtcct tcctgcccac gcgctgtggc
     4861 ctggccaccg atgtattggc gcgcaacccg cagctgaaga cggtgcagtc gccctatggc
     4921 gacgccgagg tctacatcgc gatgccggca ctgaagctcg acgtcgcgct gctgcacgtc
     4981 aacgaggccg accggctcgg caacacgctc acgcgcagcg ccgacccgat gttcgacgac
     5041 ctgttcgcgc gtgccgccga gcgctgcttc gtcagcaccg agacgctggt cgaccggctg
     5101 tcgctcgacg agcacagcgc gcgcttcaac cagttcgagc gctatctcgt gacgggggtc
     5161 gtgcatgccg cgtgcggtgc ccacccgacc tacaacgggc cggactacgg ctgggatctc
     5221 gagcacctga agcgctacaa cgccagcgcc tcggaagaag gcggctggca agcgtatgtc
     5281 gacgagttcg tcgccatcgg ggaggccgcg tatctggcca aggtcggtgg cgccgagcgc
     5341 atcgccacgc tgcagcggcc ggtgttctga cagggatacg acatgacaca gaacatcgac
     5401 atcaccctgg ccgagctgat ggccgtggcc tgctccgagg cctggcgcgg cgacggcgaa
     5461 ctgctggcgt ccggcatcgg catcctgccg cgcctgggcg cgagtctggc caagctgacg
     5521 cacagccctg aactgctgat gaccgacagc gagaccttcc tggtcgaaga gccgattccg
     5581 ctcgggccac gcggcgacta cgtgcccaaa tactcgggct acctgtcgtt ctcgcgcgtc
     5641 ttcgactgcg tctggggcgg acgccgccac gcgatgatcg ggccgacgca gatcgatcgc
     5701 tgggggcaga cgaacctgtc gtccgtcggc gactacgcca agccgaagac cgccatcctc
     5761 ggtgtgcgcg ggcttcccgg caacagcatc aaccacccga actcgctgtt cgtgccgaac
     5821 catggaccgc gggtgttcgt cgccggcgag gtcgacatgg tctcgggcgt gggctacaac
     5881 ccggcgcgct gggccgcagg gatgaagcag gactacgtcg acctgcgcct gatcgtcacc
     5941 gacctgtgcg tgatggactt cggcgggccc gaccacgcga tccgggtgac ctcgctgcat
     6001 ccgggcgtga gcttcgagca ggtccagacc gccaccggat tcccgctgct gaaggcgccg
     6061 ggccttgccg tcacgccgct tccgagcgac gcgcagctcg ccgtcattcg ccgcctcgac
     6121 ccgcacaacc tgcgcgccac ggtcatcaag aacaacccgc cggccgcgcg ccatgcgcag
     6181 tagcggcagc agcggcacga ggagcccggc atgaaccaag gtctggtcga cccgaagcag
     6241 gtcgacatcc gctacgaaac cgacacgccc gttcgctatg cggtcgaggg tccggtggcg
     6301 tggatcacga tggaccgcac cgccttcaac aacgcgcaga actcgcagat gacgtatgca
     6361 ctggacgatg cgttccgtcg cgccgtcgac gacgatgccg tcaaggtgat cgtgctcgcg
     6421 ggtgccggca agcacttctc ggccggccac gacatcggca cgccgggccg cgacgtgcac
     6481 aagagctggg agcgcaagca cctctggtac gaccacacca acaagcccgg ggccgagttc
     6541 ctctacaccc gcgagcagga ggtctacctc ggcatgtgcc ggcgctggcg tgaagcgccg
     6601 aagccgacga tcgcgatggt gcagggcgcg tgcatcgccg gcggcctgat gctcgcctgg
     6661 gtgtgcgacc tcatcgttgc ctcggacgac gcgttcttcc aggacccggt ggtgcgcatg
     6721 ggcatcccgg gcgtcgagta tttcgcccac gcgttcgagt tgccgccgcg catcgcgaag
     6781 gaattcctct tcatgggcca gcgcatgtcg gcgcagcggg ccgagcagtt cggcatggtc
     6841 aaccgcgtcg tgccgcgcag cgagctcgag aagacgaccc gcgagatggc cgtgtcgatc
     6901 agcgagatgc cgcgtctggg tctgtctctg acgaagcagg cgatcaacca cgtcgaggat
     6961 ctgcgcggca agcgcaccgc gatggatgcg gtgttccaca tgcaccactt cgcgcatgcg
     7021 cagaacgacc tggtttccgg caacagcctg gcgggcctcg acggcaaggc catggcggac
     7081 gccaacaaga agcaggccgg caaggcctga gggcacatcc cgatgagtac tccctacacg
     7141 gtagagatcg atgccggcat cgccgagctg gtcatcaacc agccgcccgt caacgcactc
     7201 gacagcgccg gctggcacgc gctggcggcg cgcatcgatg aactcggccg cgaccccgcg
     7261 gtccgcgtca tcgtgatccg cgccgagggg cgtggcttct gcgccggcgt ggacatcaag
     7321 gagctcgctg cgcatccgga gcgcatcgtt gcggtgaacg cgggcaacta cgagacgttt
     7381 cgcgcggtgc accgcaaccc ggtgccggtc atcgcggccg tgcacggctt cgtgctcggc
     7441 ggcggcatcg gcatcaccgg cgcggccgac atcgtggtcg cctccgagtg cgctcgcttc
     7501 ggcgtgcccg aggtggaccg cggcgcgatg ggcggtgcag cgcacctgca gcggctcttc
     7561 ccggtgcaga aggtgcgggc gatgtatttc accggcgaca tgatcgacgc ggccgaggcc
     7621 taccgcctcg gcgcggtgga gaagctggtg ccgcgcgagc agttgcgcga gacggcgctg
     7681 gccatcgccc gcaagatcgc cgcgaagagt ccggcgatgg ttcgcctcgc gaaggagtcg
     7741 ctcaacggca tcgaggacgg cgatctcgaa gccaagtacc gctgggagca gggcttcacg
     7801 ctgcaggcct acatgagccc ggattccaac gagacgcggc aggcattcgt caccaagcgc
     7861 gaagccaggt tctgagaagg cggaggagcg acacgtgcaa ctcgagtaca ccgaccagca
     7921 gcgcgccttt cggctcgagg tccgcacgtg gctgcaggcc cgtgtgccca ggcagccgct
     7981 gcagagcttc gacaccgagc agggcttcga gcagcaccgg caatgggaag cgacgctgaa
     8041 ctccggccgc ttcgcgatgg tcacctggcc caaggaactg ggcggccgcg gctgcgacct
     8101 catcgagtgg ctgatcttcg aggaagagta ctggcgcgcc ggggcaccgc tgcgcgtcaa
     8161 ccagaacggc atcttcctgc tcggccccac actcatggac tacggcacgc cggaacagaa
     8221 ggcgcgcttc ctgccgcgca tggcggctgg gcaggacgtc tgggcgcagg gctggtcgga
     8281 gccgaacgca ggctccgaca tggcagcgat ccgctgcaag gcgacccgca acaacgggca
     8341 ctacatcctg aacggccaga agatctggtc cacccgcgcc gtgtgggccg actggttgtt
     8401 cggcttgttc cgcagcgacc ccgattcgca gcgtcaccac gggctgacct ttctgctcgt
     8461 gccgctgaag acgcccggca tcacggtgcg gccgatccgc cagctcaacg gcctgccggg
     8521 gttcgccgag atcttcttcg acgacgtcaa ggtgccggtc gaaaaccgcc tgcacgccga
     8581 gggcgaaggc tggcacgtgg caatggccac agccggcttc gaacgcgggc tcatgctgcg
     8641 ttcgccggcg cgcttccagt ccaccgcggc cgcgctggtc ggcctctaca acaagcatcg
     8701 tgaatcggcg gatcgcgacc cggccgtgcg cgaggccgtg ctgcaggcct ggatgcaggc
     8761 ggaagcctat gcgctcgcaa cctacggaac cgccagtcgc ctgatgaagg gcggcgcgat
     8821 cggcgccgaa tcgagcacga acaagatctt ctggtcgacg ctcgaccagc gcatgcacga
     8881 gacggcgatg cacatcctgg gtgcccgtgc cgaactgctg ccgcacgcgc cggccgccgg
     8941 cgatgtcggc aactggctcg acggcttcct gttcgcgcag gcgggcccga tctatgccgg
     9001 caccaacgag atccagcgca acatcatcgc cgagcggatg cttggcatgc cgcgctgaag
     9061 gaagcacccg atggacttca ccttcaccaa cgaccagtcg ctgtttcgcg atgccattcg
     9121 cagctacctg atggtcgagg cgaccccgga actgctgcgc tcggtctggg agacgccgtc
     9181 cggccgctcc gacccggcgt tcgaaaagtt cgccgagcag gggctcaccg gcttgtcgat
     9241 ccccgaggcc gagggaggcc tcggctgcaa cgaactcgac tgggtgctga tggcccagga
     9301 actgggctac tacggcgtgc ccgattcgct gctgtcgacc gcttgcctgg ccgtcgggct
     9361 gttgacggcc ttgccgggcg atgcgccggt ccgcaaggcg tggttgccgc gcatcctcgc
     9421 gggcgatgcc cggatcgcgg tcggccatcc gatcagcgca ctcgtggcgg acgcccatgt
     9481 ggccgacctg ctgttgctgc accatgccgg cgaagtgcac gccgtgccgc gctcagaggt
     9541 cgatctggtc ctgaatccga gcgtcgatcc ggcgcggcgt ctgcacacgg tggcgtggaa
     9601 gccatccggg cgcacgcgca tcgccgacgc cgacgccggg cgcgtgctgt gggacgacat
     9661 gttcgagcgt ggcgccctcg tcacggcggc gcaatccatc ggtctgaccc ttcgcatgat
     9721 ggatctggcg gtggactaca gcgtgcagcg caagcagttc ggcaaaccga tcggatcgtt
     9781 ccaggcggtc aagcatctga tggcggatgt cgccgtcgcg gtcgagttcg ccaagccggt
     9841 cgtgtaccgg gccgcccatg cgctggcgca tcgcctgccc catcgtgcag tccatgtgtc
     9901 gcacgccaag ctggccgccg atgaagcggc gtggctcgca gcacgcaaca gcattcaggt
     9961 gcatggggcg atgggctaca cctgggaggt cgacctgcag attttcatga agcgcgcctg
    10021 gtcgctcggt gcgagctggg gcgaccgcgg cctgcacaag acccgcgtgt ccgacttcgt
    10081 gctcgcggac ggggctcccc tgggccccgg caacaccttc atttccacga accattgagg
    10141 caacccatgg ctgaagccta tatcgtcgac gcgttgcgca cgcccaccgg caaacgcggt
    10201 ggagcgctcg cccccgtgca tgccgccgac ctcggcgcct tcgtattgaa gaccctgatg
    10261 gagcgcaacg cggtgccccc cggtgatgtc gacgacgtca tcttcggctg cgtcgatgcg
    10321 gtgggcccga acgccggcgt catcagccgc acctcctggc tggcggccgg gcttccggac
    10381 cacgtgcccg gcacggtgat cgaccgcgca tgcggttcgt cacagcaggc ggttcacttc
    10441 gcggaccagg ccgtgatgag cggcacccag gacgtggtgg tggccggtgg tgtgcagaac
    10501 atgagccagg tgcccatcgc ctatgccatg gtggcggcac agccgctggg gttcaccgac
    10561 ccgttctcgg gcagcaaagg ctggaaggaa cgcttcggcg acaagccggt gaaccagttc
    10621 tatgccgcgc agatcattgc cgacaagtgg ggctgctcgc gcgatgacat ggaagccttt
    10681 gccgccgaga gccacacccg cgcgttgcgc gcgcaggccg aggggcgctt cgcgcgcgag
    10741 atcgtggcgc tgaacggctt gtcgcacgac gaaaccgcgc gcgcgccgaa ccgcgagaag
    10801 atggccacgc tgcagccggt ggatgcgaag taccccagca tcacggccgc cgtgtccagc
    10861 tcgacctgcg acgccgcggc cgccttgctg atcgtgtcgg aagcggccct caagcgctac
    10921 ggcctgaagc cgcgcgcgcg catccatcac ctgagcgtgc tcgccgctga cccgatctgg
    10981 catctgaccg cgccgatccc cgccaccgcc gcggccctga agaaggccgg aatgaagatc
    11041 gatgacatcg accttgtcga gattaacgaa gccttcgcgt cggtcgtgat ggcctggctg
    11101 aaggagaccg gctacgcgca tgccaggacc aacgtcaacg gcggcgcgct cgcgctcggc
    11161 catccgctgg gcgcttccgg cgcccggctc atgacgacgc tgctgcacga actcgaacgc
    11221 accggcggcc gctacggcct gcagacgatg tgcgaaggcg gtggccaggc caacgtcacg
    11281 atcatcgagc ggctctgatc ctcaccttgc actgaagaca cacaccatgg gaatttgcga
    11341 caaacgcagc gtcatcgtca ccggcgcggg gggcggcctc ggccgcgccc atgcgctggc
    11401 gttggcgcaa gccggcgcca gcgtgctggt caacgacatc cgccgtgagg cggccgaagc
    11461 cgtggtggtc gaaatcgtgg ccgcgggcgg caagggcatc gccaacagcg acgacatcgc
    11521 cagcatcgcg ggcgcccagc gcatcatcga tgcagcggtg gctgatttcg gcgatgtgca
    11581 ggcggtggtc aacaacgccg
//
LOCUS       DATMJB010000167         3259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_411723, whole genome shotgun sequence.
ACCESSION   DATMJB010000167 DATMJB010000000
VERSION     DATMJB010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411723"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="VNU48_03780"
     CDS             <1..1301
                     /locus_tag="VNU48_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HWI10428.1"
                     /translation="TGMPKLFASQIVIHDRDTGASIPATVKVNEPAFHRGVAIYQSSF
                     DDGGSSLKLRGIPMAAGGRPFDIDGVVGGNTQISNGDAKLTLEFTGLRVINVENLGGN
                     AGAGGATDVRKVDLVSSFTDHLGSGAKGDNKKTLRNVGPSISYKLRDAAGQAREFNNY
                     MLPVELDGQRVFLAGARDKPDQEMRYLRIPADDQDSIAGWARLRAGLLDPALRAKAAA
                     RYVALATPRDKPEMAAQLTATTNSALAMFAGADPARPKDNADPGKPRGGLQAISGFIE
                     GSVPEAQRGRISEVLLRILNGSLFELLNLTREDAGLTALQPDDKTQAFMTQSVLSLSD
                     SFFYPAPVLFELADFKQVQASVFQVARAPGQKLVYLGAVLLIVGVFAMLYIRERRLWI
                     WLVPAGAGTKITTAMSTTRRTLDADAEFERLKRQVLKEAP"
     gene            1298..2668
                     /gene="ccsB"
                     /locus_tag="VNU48_03785"
     CDS             1298..2668
                     /gene="ccsB"
                     /locus_tag="VNU48_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HWI10429.1"
                     /translation="MNTTTLKTMTLGRPAGPIGFFSGRSLFDWLFAALVLAGGAYAFS
                     RYAAAMDVYEKGILLGALPVAVLLGWFWGSLRVLMLGVAAAALFAVSLYNRQMDGFGA
                     DLAQGDKVFMLKYFLSSQSAIMWMSVLFFMSTTFYWIGFAAKTENNTAVRIGSGLTWA
                     AVFMALTGTVVRWFESHQIGPDIGHIPVSNLYEVFVLFCWLTSLFYLYYEQHYKIKSL
                     GAFVMLVVSSAVGFLIWYTVARGASEIQPLVPALQSWWMKLHVPANFIGYGSFALSAM
                     VALAYVLKNSTTRALWIGLLGVPGVVIALPLLGFALFGGLSPEGLGSVAKAGRMVVGT
                     MGFFGFTILARQWINARTPSAEIADDLMYKSIAVGFAFFTIATILGAFWAAEAWGGYW
                     SWDPKETWALIVWLNYAAWLHMRLMKGLRGNVSAWWALTGLVVTSFAFLGVNMFLSGL
                     HSYGEL"
     gene            2761..3259
                     /locus_tag="VNU48_03790"
                     /pseudo
     CDS             2761..3259
                     /locus_tag="VNU48_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229000.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
BASE COUNT          508 a         1101 c         1124 g          526 t
ORIGIN      
        1 acaccggcat gcccaagttg ttcgcgagcc agatcgtgat ccacgaccgc gacaccggcg
       61 cctcgatacc ggccacggtc aaggtcaacg aaccggcatt ccaccgcggc gtcgcgatct
      121 accagagcag cttcgacgac ggcggctcgt cgctgaagct gcgcggcatc ccgatggccg
      181 ccggcggccg tccgttcgac atcgacggcg tcgtcggcgg caacacgcag atcagcaacg
      241 gcgacgcgaa gctgacgctc gagttcaccg ggctgcgcgt catcaacgtc gagaacctcg
      301 gcggcaatgc gggcgcgggc ggcgccaccg acgtgcgcaa ggtcgacctc gtcagctcct
      361 tcacggacca cctcggctcg ggcgccaagg gcgacaacaa gaagacgctg cgcaacgtcg
      421 ggccgtcgat cagctacaag ctgcgcgacg ccgccggcca ggcgcgcgag ttcaacaact
      481 acatgctgcc agtcgagctc gacggccagc gcgtcttcct ggccggcgca cgcgacaagc
      541 cggatcagga gatgcgttac ctgcgcatcc ccgccgacga ccaagacagc atcgccggct
      601 gggcccggct gcgcgcgggc ctgctcgacc cggccctgcg cgcgaaagcc gcggcgcgct
      661 acgtcgcgct ggccacgccg cgcgacaagc ccgagatggc cgcgcaactc acggccacca
      721 ccaactcggc gctggcgatg ttcgccggcg ccgacccggc gcgcccgaag gacaatgccg
      781 acccgggcaa gccgcgcggc ggcctgcaag ccatctcggg tttcatcgaa ggcagcgtgc
      841 ccgaagccca gcgcgggcgc atctcggaag tgctgctgcg catcctgaac ggcagcctgt
      901 tcgagctgct gaacctgacg cgtgaagacg caggcctgac ggcactgcag cccgacgaca
      961 agacgcaggc cttcatgacc caatcggtgc tgtcgctgtc cgacagcttc ttctacccgg
     1021 cgccggtgct gttcgagctg gccgacttca agcaggtgca ggccagcgtg ttccaggtcg
     1081 cgcgcgcgcc gggccagaag ctcgtctacc tcggcgcagt gctgctgatc gtgggcgtat
     1141 tcgcaatgct ctacattcgc gagcggcgcc tgtggatctg gctcgtgccg gcgggtgccg
     1201 gaaccaagat caccaccgcg atgtccacca cgcgccgcac gctggacgcc gatgccgagt
     1261 tcgagcgact gaagcggcag gttctgaagg aagcgccatg aacaccacca cgctgaagac
     1321 gatgacgctg ggccgcccgg cgggcccgat cggcttcttc tcgggtcgct cgctgttcga
     1381 ctggctgttc gccgcgctcg tgctggccgg tggcgcctac gcgttctcgc gctatgccgc
     1441 ggcgatggac gtgtacgaga aaggcatcct gctcggcgcg ctgccggtcg cggtgctgct
     1501 gggctggttc tggggctcgc tgcgcgtgct gatgctgggc gtggccgcgg ccgcgctgtt
     1561 cgcggtcagc ctgtacaacc gccagatgga cggcttcggc gccgacctcg cgcagggcga
     1621 caaggtcttc atgctgaagt acttcctgtc gagccagagc gcgatcatgt ggatgagcgt
     1681 gctgttcttc atgagcacca cgttctactg gatcggcttc gcggcgaaga ccgagaacaa
     1741 caccgcggtg cgcatcggct cgggcctgac ctgggcggcc gtgttcatgg cgttgaccgg
     1801 caccgtggtg cgctggttcg agagccacca gatcggcccc gacatcggcc acattccagt
     1861 gtccaacctg tacgaagtgt tcgtgctgtt ctgctggctg acctcgctgt tctacctgta
     1921 ctacgagcag cactacaaga tcaagtcgct gggcgcgttc gtgatgctgg tggtcagcag
     1981 cgcggtcggc ttcctgatct ggtacacggt ggcgcgcggc gcgagcgaga tccagccgct
     2041 ggtgccggcg ctgcagagct ggtggatgaa gctgcacgtg ccggccaact tcatcggcta
     2101 cggcagcttc gcgctgtcgg cgatggtggc gctggcctac gtgctgaaga actcgacgac
     2161 gcgcgcgctg tggatcggcc tgctcggcgt gcccggtgtc gtgatcgcgc tgccgctgct
     2221 gggcttcgcg ctgttcggcg gcctgtcgcc ggagggcctg ggctcggtcg cgaaggccgg
     2281 gcgcatggtg gtcggcacga tgggcttctt cgggttcacg atcctggcgc gccagtggat
     2341 caacgcgcgc acgccgagcg ccgagatcgc cgacgacctg atgtacaagt cgatcgccgt
     2401 gggcttcgcg ttcttcacga tcgccaccat cctgggtgcg ttctgggccg ccgaagcctg
     2461 gggcggctac tggagctggg accccaagga aacctgggcg ctgatcgtct ggctcaacta
     2521 cgcggcctgg ctgcacatgc ggctgatgaa ggggctgcgc ggcaacgtct cggcctggtg
     2581 ggcgctgacc gggctggtcg tcaccagctt cgcgttcctc ggagtgaaca tgttcctgtc
     2641 ggggctgcac tcgtacggcg aactgtgagc tgacctttcg gtggcgccgg ccccggcgtc
     2701 cccggatgtc agaagcccgt cgtgcggcga cggaccggca agacccgagc gagcaccgcc
     2761 atgcacttcc tgcgcgacca aggattcgac cacgcccacg cctgcgagat cacgccgcgg
     2821 gcgacctacg agcgccggcg cgaactgctg aagctgctcg gcagcggcgt ggccggcgcg
     2881 gccctggcga gttgggcggc gcgcgaggcg caggcgcagg cgcaggccgc acgcccagcc
     2941 aggctcgccg cgctgcccgg ggcgcgcagc gcggtctccg gcgccgcgag ccgaaggtct
     3001 acgacatcga cgaactgctc agggtcgcgc cgatggagga gcgcatctac cgcctgcggt
     3061 gtgtcgaagg ctggtcgatg gtgatcccgt gggtcggcta ttcgctggcc gaactgatca
     3121 gaagagtcga gccgaccggc aacgcgaagt acgtcgagtt cgtgaccctg gccgacccga
     3181 agaccatgcc ctacgtcggc ggctcacgcg tgctcgactg gccttatgtc gaaggcctgc
     3241 gcctggacga ggcgatgca
//
LOCUS       DATMJB010000168         6640 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_215346, whole genome shotgun sequence.
ACCESSION   DATMJB010000168 DATMJB010000000
VERSION     DATMJB010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6640
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215346"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VNU48_03795"
     CDS             <1..927
                     /locus_tag="VNU48_03795"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWI10430.1"
                     /translation="GMDAVGTAARAIKSGEAQLMLAGGVESMSRAPFVMPKAESAFSR
                     ANAVYDTTIGWRFVNKRMKELYGVDSMPETAENVATDFKIERAAQDRMALASQLKAVA
                     AQKSGFFDAEITPVSVAQKKGDPISVTKDEHPRETSLEALAKLKGVVKPDGTVTAGNA
                     SGVNDGACALLIAGEAAAARHGLTPKARVVGMATAGVAPRIMGFGPAPATRKVLELTG
                     LALAQMDVIELNEAFAAQGLAVLRDLGVADDDARVNPNGGAIALGHPLGASGARLVTT
                     AVNQLQRINGRYALCTMCIGVGQGIALIVERV"
     gene            complement(946..1236)
                     /locus_tag="VNU48_03800"
     CDS             complement(946..1236)
                     /locus_tag="VNU48_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10431.1"
                     /translation="MSEDYGMESPGAHRHTQHQAPARYLIVIDAAGSMVARLFQATRE
                     QVAEFDAGSEEVAVMLKGLAPARGADGADWDRALQGHSAAERRAADVYTLDL"
     gene            1546..5952
                     /locus_tag="VNU48_03805"
     CDS             1546..5952
                     /locus_tag="VNU48_03805"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA14 domain-containing protein"
                     /protein_id="HWI10432.1"
                     /translation="MALLLSCGGGGEDRAPDAAPAARVGGDRKKIQSFVPPTTIPPEA
                     NIKGMWSPVYPWPIIAVHSVLLPDGRVLTYGSDPTGLQTAHANVDVWDSSAAPDTNHL
                     LLPNGTGTDLFCSSQLVLPQTGSVFIAGGDVWNGTSVNNGPNNNSNLFDSASTSLARG
                     PNMNRARWYSSSTTLISGETYIQGGSGGGDRPEIRGVDGSFRLLTGIDTSSIGTSFPR
                     NFVAPDGRIFGYDPGNGRMYFVNPTGSGAIAFSTSFNAAYSGGWYSSTAMYRPGRILQ
                     IGGNSNGAYTIDITGGTPVVAPTQSMSSTRAWVNATILADGKVVATSGSAVAGSATGY
                     NNIAETWDPATGQWLQGAVAQKMRLYHSNALLLPDGSVLVAGGGAISPSPPSGDPNTN
                     NLNAEIYYPPYLFSAGGVRAARPAIATAPSWIDIGKTFSIDVTDAASISRITLVKTGS
                     SSHSFNLDQRFLDVTFKANGLHVAVQAPTRAGDAPPGYYMMFVFNEVGVPSVAKIVRI
                     GIAADPNPAINPVLGNPGAQTSAVGNAVQLNLVASDPNGDVLTYAATGLPPGLSIDTA
                     SGRISGTVTTAGNFNAVVSATDGVNTASAGFVWTVQASAPLTLDVPPAPVFVVIGGVA
                     TYTASAVGGPNLRYQWNFGDGTATTSWSTSPTTTHTYAAPGVYSVTVSVTDDSQGVQS
                     RSFSQAVYLPTTAKSPGASSNLLVEKPPSGNPRLWVVNQDNDSVSAFDAVTYAKLGEV
                     AVGTAPRAIALAQNGLIWVTNKRAASISVIDPATRTVTRTLTLPRGSQPFGIVTASVG
                     NAAYVVLEGGGQLLKFDTSTYAQLGSATIGLNARHVSLSADGANLYVSRFITPPLPGE
                     STAVVTTTASTGGEVIQLNATTLAIVRTIVLQHSEKPDAENQGRGIPNYLGAAVISPD
                     GSQAWVPSKQDNIKRGGLRDGTGLNFQSTVRAVSSRIDLATNAEDLAARIDHDNASLA
                     SAAVYDPRGVYLFVALETSREVAVVNAFSRAEMFRISVGRAPQGLAVSADGTALFVSN
                     FMDRTVSVRSLTPLLVNGQYDLPLAATLSSVGIEKLGASVLLGKQHFYDARDTRLARD
                     GYMSCASCHNDGGHDGRVWDLTSLGEGLRNTVALRGRGGAQGFLHWSNNFDEVQDFEG
                     QIRMLAGGTGLMSNALFNAGTRNTPLGDRKAGLSADLDALAAYVASLNAFDPAPARPS
                     AGALSTSAAAGKAVFSAMNCAACHSGAAFTGSGANTLVDIGTLKQPSSGSRLYGPLTG
                     IDVPTLRDVWATAPYLHDGSAPTLDIAVRAHAGVVISDPDLAALTQYLREIGGDEPAA
                     AAPAGNGTGLKGSYFNNLTLSGTAVLTRIEAVNFDWGGHSPGGGVSSNNFSARWTGTL
                     IAPSTGTYRFQTYSDAGARLWVNGVQLVNNWVDHKPTTDTSVGMNLVAGQRVPIQVDY
                     YERSGSAVMQLRWLAPGDAAYVPIPASRLASP"
     gene            complement(5993..6523)
                     /locus_tag="VNU48_03810"
     CDS             complement(5993..6523)
                     /locus_tag="VNU48_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWI10433.1"
                     /translation="MLKTIAVVVAVLIAGVLIVASMKPDSFSVQRSATIKAPPDKVFA
                     VLNDFHRWTEWSPWEKLDPLMKRTLGGPASGKGATYAWEGNSKAGAGRMEIIESVPAR
                     RIGMQLDFIKPFEGHNVAEFTLTPQGDATQVNWQMRGPAPFIAKLMQVFVSMDTMIGK
                     DFEEGLANLKAVAEKS"
BASE COUNT         1093 a         2360 c         2193 g          994 t
ORIGIN      
        1 ggcatggacg cggtcggcac ggcggcgcgc gcgatcaaga gcggcgaggc ccagctgatg
       61 ctggccggcg gcgtcgagag catgagccgc gcgcccttcg tgatgccgaa ggccgagagc
      121 gcgttctcgc gcgccaacgc ggtctacgac accaccatcg gctggcgctt cgtcaacaag
      181 cgcatgaagg aactctacgg cgtcgactcg atgcccgaga ccgccgagaa cgtcgcgacc
      241 gatttcaaga tcgagcgcgc ggcgcaggac cgcatggcgc tggcctcgca gctgaaggcg
      301 gtggcggccc agaagagcgg cttcttcgat gccgagatca cgccggtcag cgtcgcgcaa
      361 aagaagggcg accccatcag cgtcacgaag gacgagcatc ctcgcgaaac cagcctcgag
      421 gcgctcgcga agctcaaggg tgtcgtcaag cccgacggca ccgtgacggc cgggaatgcc
      481 tcgggcgtga acgacggcgc gtgcgcgctg ctgatcgccg gcgaggccgc tgccgcgagg
      541 cacggcctga cgccgaaggc gcgcgtggtc ggcatggcca ccgccggcgt ggcgccgcgc
      601 atcatgggct tcggcccggc tccggcgacg cgcaaggtgc tcgagctgac cggcctggcg
      661 ctcgcgcaga tggacgtgat cgaactcaac gaggccttcg ccgcgcaggg cctggcggtg
      721 ctgcgcgacc tcggcgtggc cgacgacgac gcgcgcgtga acccgaacgg cggcgcgatc
      781 gcgctcggcc acccgctcgg cgcgagcggc gcgcggctcg tgacgaccgc ggtcaaccag
      841 ctgcagcgga tcaacggccg ctatgcgctg tgcacgatgt gcatcggcgt gggccagggc
      901 atcgcgctga tcgtcgaacg ggtctgatgc gcggcgccgg gccggctaca ggtcgagcgt
      961 gtagacgtcc gccgcgcgcc gctcggccgc gctgtggcct tgcagcgcgc gatcccagtc
     1021 ggcgccgtcg gccccgcgcg cgggcgccag gcccttgagc atcaccgcga cttcctcgct
     1081 gccggcgtcg aactcggcga cctgctcgcg ggtcgcctgg aacagccggg ccaccatgct
     1141 gccggccgca tcgatcacga tcaggtagcg cgccggcgcc tggtgctgcg tgtggcggtg
     1201 cgcgccgggg gactccatgc cgtagtcttc gctcattcga tcatcttgcc acgtgcatgg
     1261 tgcgggcttc gtcaatcgaa agcggccggc ccatgaaatc actcggcaaa cgcgctgacg
     1321 gtgcacgcct ggagcgcatg cgccgcgcgc gtgccgcggg caccggcgac caacagcatc
     1381 tccccgcgga cgcagcagtc caccaaatgt gggattgccc gcggcgcaga agcggctaca
     1441 tttccttacg tgtggcagcg caacgccgcc gcgagaagac aacaaggagg ccagagcatg
     1501 tgtgaacgca cgccgcgccg tcgcattctg gtctcgttgc ccctcgtggc gcttctgctg
     1561 tcctgcggcg gcggcggtga agaccgcgcc ccggacgccg ccccggcggc gcgtgtcggc
     1621 ggcgaccgga agaagatcca gtccttcgtc ccgccgacga ccatcccccc ggaggccaac
     1681 atcaaaggca tgtggtcgcc ggtctacccc tggcccatca ttgcggtgca ctcggtgctg
     1741 ctgccggatg gccgcgttct gacctacgga tcggacccca ccggcctgca aactgcccat
     1801 gcgaacgtcg atgtctggga cagcagcgcc gcgcccgata ccaaccacct gctgctgccc
     1861 aatggcaccg gcaccgatct cttctgcagc tcgcagctcg tgctgccaca gacgggcagc
     1921 gtgttcatcg ccggcggcga cgtctggaac ggcaccagcg tcaacaatgg tccgaacaac
     1981 aacagcaatc tgttcgactc ggccagcacg tccttggcgc gcggtccgaa catgaaccgg
     2041 gcgcgctggt attcatcgtc gacgacgctg atcagcggcg agacctacat ccagggcgga
     2101 tccggcggcg gagatcgacc cgagatccgc ggcgtcgacg gcagcttccg gctgctgacc
     2161 ggcattgaca cgagcagtat cggcacctcg ttcccacgca acttcgtcgc ccccgacggc
     2221 cgcatcttcg gctacgaccc gggcaacggc cgcatgtatt tcgtcaaccc gacgggcagc
     2281 ggcgctatcg cgttctcgac cagcttcaac gcggcctaca gcgggggatg gtactcgagc
     2341 acggcgatgt accggccggg gcgcatcctg cagatcggcg gcaattccaa cggcgcctac
     2401 acgatcgaca tcacgggcgg cacgccggtc gtcgccccga cgcagtcgat gtcgagcacg
     2461 cgcgcctggg tgaacgcgac gatcctcgcc gacggcaagg tggtcgccac cagcggcagt
     2521 gccgtcgccg ggtcggccac gggctacaac aacattgccg agacctggga cccggcgacc
     2581 ggccagtggc tgcagggcgc agtcgcgcag aagatgcgcc tgtaccactc gaacgcgctg
     2641 ctgctgcccg acggcagcgt gctcgtcgcc ggcggtggcg cgatctcacc ttcgccaccc
     2701 agtggcgacc cgaacaccaa caacctgaat gccgagattt actacccacc gtacctgttc
     2761 tccgcgggcg gcgtgcgcgc cgcacgtccg gcgatcgcga ccgcgccgtc ctggatcgat
     2821 atcggcaaaa ccttcagcat cgacgtcaca gacgccgcga gcatcagccg catcacgctc
     2881 gtcaagaccg gctcgtcgtc gcacagcttc aacctcgacc agcgcttcct cgacgtgacc
     2941 ttcaaggcga acggcctgca cgtcgcggtc caggcgccga cgcgcgccgg cgacgctcca
     3001 cccggctact acatgatgtt cgtcttcaac gaggtcggcg tgccctccgt ggccaagatc
     3061 gtccgcatcg gcatcgcggc ggacccgaat cccgcgatca accccgtgct tggcaacccg
     3121 ggtgcgcaga ccagtgcggt cggcaacgcg gtccagctca acttggtcgc gagcgacccg
     3181 aatggcgatg tgctgaccta cgccgccacc gggttgccgc cgggcctgtc gatagacacg
     3241 gcgagcggcc gcatcagcgg tacggtgacg acagccggca acttcaacgc cgtcgtgtcg
     3301 gcgaccgatg gcgtcaacac cgcgtcggcg ggcttcgtgt ggaccgtgca ggcttctgcc
     3361 ccgctgacgc tcgacgttcc gccggcgccg gtgttcgtgg tgatcggtgg cgtggcgacc
     3421 tacaccgcca gcgcggtcgg cgggcccaac ctgcgctacc aatggaactt cggcgacggc
     3481 accgcgacca cgtcctggtc gacctcgcca acaacgacgc acacctatgc ggcaccgggc
     3541 gtctacagcg tgacggtcag cgtcaccgac gacagccagg gggtgcaaag ccgcagcttc
     3601 tcccaggcgg tgtacctgcc gaccacggcc aagtcgccgg gtgcttcgag caacctgctg
     3661 gtcgagaaac cgcccagcgg caacccgcgc ctgtgggtgg tgaaccagga caacgacagc
     3721 gtcagcgcgt tcgacgccgt gacgtacgcc aaactcggtg aagtcgccgt gggcacggcg
     3781 ccgcgcgcga tcgcgctggc gcagaacggg ctgatctggg tgaccaacaa gcgtgcggcg
     3841 tcgatcagcg tgatcgaccc ggcgacgcgc acggtcacgc gcacgctgac gctgccgcgc
     3901 ggttcacagc cattcggcat cgtcacggcg tcggtcggca acgcggccta tgtcgtgctc
     3961 gagggcggcg gccagctgct caagttcgac acgtcgacct acgcgcagct cggcagtgcg
     4021 accatcgggc tgaacgcgcg gcatgtgtcg ctgtcggccg acggtgccaa cctgtacgtg
     4081 tcgcgcttca tcacgccacc gctgcccggc gagagcacgg ccgtggtcac caccaccgcg
     4141 agcaccggtg gcgaggtcat ccagctgaat gccacgaccc tcgccatcgt gcgcaccatc
     4201 gtgcttcagc acagcgagaa acccgacgcg gagaaccagg gtcgcggcat tcccaactac
     4261 ctcggcgcgg cggtcatctc gcctgacggc agccaggcct gggtgccgtc caagcaagac
     4321 aacatcaagc gcggtggtct gcgcgacggg accgggctca atttccagag caccgtgcgc
     4381 gccgtcagct cgcgcatcga cttggccacc aacgctgaag acctcgccgc ccgcatcgat
     4441 cacgacaacg ccagtcttgc cagcgccgcg gtctacgacc cgcgcggcgt ctacctgttc
     4501 gtcgcgctcg agaccagccg cgaggtggcg gtcgtcaacg cgttctctcg tgccgagatg
     4561 ttccggatca gtgtcggccg cgcgccgcaa ggcctggcgg tctcggccga cggcaccgcg
     4621 ctgttcgtca gcaacttcat ggatcgaacc gtcagcgtgc gcagtctcac gccactgctg
     4681 gtcaatgggc agtacgacct cccactcgcg gccacgttga gttccgtggg catcgagaag
     4741 ctcggcgcca gcgtgttgct cggcaagcag cacttctatg acgcgcgcga cacacgcctt
     4801 gcccgcgacg gctacatgag ctgcgcgagc tgccacaacg acggcggcca cgacggccgc
     4861 gtctgggact tgaccagcct gggcgagggc cttcgcaaca ccgttgcgct gcggggtcga
     4921 ggcggcgcac aaggcttcct gcactggagc aacaatttcg acgaggtgca ggacttcgag
     4981 ggccagatcc ggatgctcgc aggcggcacc ggcctgatgt ccaacgcgct gttcaatgcg
     5041 ggcacgcgca acacgccgct gggcgatcgc aaggccggcc tgtcggccga tctcgatgcg
     5101 ctggccgcgt acgtcgcctc gctgaacgcc ttcgatccgg cgccggcgcg gcccagcgca
     5161 ggcgcgctgt cgaccagcgc ggccgccggc aaggcggtgt tcagcgccat gaactgcgcg
     5221 gcgtgccaca gcggcgcggc cttcaccggc agcggcgcca acacgctggt cgatatcggg
     5281 acactgaagc agccctcgag cggatcccgc ctttacggcc cgttgaccgg catcgacgtg
     5341 ccgacccttc gcgacgtgtg ggcgaccgcg ccgtatctgc acgatggctc ggcgccgacg
     5401 ctggacatcg cggtccgcgc ccatgccggc gtcgtcatca gcgaccccga tctcgcggcg
     5461 ctgacccaat acctgcgcga gatcggtggc gacgagcccg ctgcggcggc acccgccggc
     5521 aacggcaccg gcctgaaagg ctcctacttc aacaacctca ccctctccgg cacggccgtg
     5581 ctgacacgca tcgaggcggt caatttcgac tggggcgggc actcacctgg cggcggtgtc
     5641 agctcgaaca acttctcggc gcgctggacc ggcacgctca tcgcgccgag caccggcacc
     5701 tatcggttcc agacctattc cgacgccggt gcgcgtctgt gggtcaacgg cgtacagctc
     5761 gtcaacaact gggtcgacca caagcccacc accgacacca gcgtcggcat gaacctcgtc
     5821 gccggccagc gcgtgccgat ccaggtggac tactacgagc gcagcggcag cgcggtgatg
     5881 caattgcgct ggctcgctcc gggcgatgcg gcctacgtcc cgatcccggc gagtcggctt
     5941 gcctcgccgt agggggcctc gggtcggcgg ccgagcagaa agaccggcag ggctagcttt
     6001 tttcggcgac ggccttcagg ttcgccaagc cctcttcaaa gtccttgccg atcatcgtgt
     6061 ccatgctgac gaacacctgc atcagcttcg cgatgaacgg cgccggcccg cgcatctgcc
     6121 agttcacctg ggtcgcgtcg ccttgcggcg tgagcgtgaa ttcggcgacg ttgtggcctt
     6181 cgaagggctt gatgaaatcg agctgcatgc cgatcctgcg cgccggtacc gattcgatga
     6241 tctccatgcg gcccgcgccg gccttgctgt tgccttccca ggcataggtc gcgcccttgc
     6301 cgctggcggg gccgccgagc gtgcgcttca tcaaagggtc gagcttctcc cacggcgacc
     6361 attcggtcca gcggtggaag tcgttcagca ccgcgaagac cttgtccggc ggcgccttga
     6421 tcgtcgccga gcgctgcacg ctgaaactgt cgggcttcat cgatgcgacg atcagcacgc
     6481 cggcgatcag cacggcgaca acgacggcga tggtcttgag catggtgtgt tcctccctgg
     6541 aagccgaaaa cgtagcggat gcagccggcg ctgtccaccg acggcgccgg cgcaccgtca
     6601 ggcgggcgtg agcgcgagcg cgcgtgcgat ccggtgccgc
//
LOCUS       DATMJB010000169         2962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_866737, whole genome shotgun sequence.
ACCESSION   DATMJB010000169 DATMJB010000000
VERSION     DATMJB010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866737"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /gene="pyrG"
                     /locus_tag="VNU48_03815"
     CDS             complement(<1..193)
                     /gene="pyrG"
                     /locus_tag="VNU48_03815"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484090.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HWI10434.1"
                     /translation="MTKYVFVTGGVVSSLGKGIASASLGAILESRGLKVTLIKLDPYL
                     NVDPGTMSPFQHGEVFVTDD"
     gene            complement(241..1056)
                     /locus_tag="VNU48_03820"
     CDS             complement(241..1056)
                     /locus_tag="VNU48_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI10435.1"
                     /translation="MKTIVFSHANGFPAGTYRLIFEAWRAAGFEVHAIEKYGHDPRFP
                     VTSNWPHLRAQLIDFIEHAAGGPAYLVGHSLGGYLSLMVASRRPDLASGLVMLDSPLL
                     SGWKARAVQVAMATGIGERFSPGHVSKRRRQQWDSADAAFEHFAAKPMFARWAPGMLR
                     DYIASGTEPHGTLHRLSFHRDIETAIYNTLPHHLARVLRAHPLQCPAAFVRGSASEEV
                     RQFGLRATERLVRGRIRTIEGSHLFPLERPAQTAAEVLQLIESMRAGAESTHL"
     gene            complement(1053..1877)
                     /locus_tag="VNU48_03825"
     CDS             complement(1053..1877)
                     /locus_tag="VNU48_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HWI10436.1"
                     /translation="MTYCVGIKLDAGLVFMSDSRTNAGLDQISTYRKMMVYEKPGDRF
                     MVMLSAGNLSISQSVREILQVEKIEDRDGAPVTIWNATSMFDAVRVLGAAVRRVYEQD
                     GASLAAAGVDFNASMIFGGQIKGEAMRLFLVYSAGNFIEATRETCYFQVGESKYGKPV
                     LDRMITPATPLDEAAKCALVSMDSTLKSNLSVGLPLDLLVYSAHALQTDRIVCVDDHN
                     PYFQMIRGNWGLKLREVFESLEDPQWGTGPTTVPLCVPSPRYEPMKKITHPDEKIV"
     gene            complement(1874..>2962)
                     /locus_tag="VNU48_03830"
     CDS             complement(1874..>2962)
                     /locus_tag="VNU48_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830425.1"
                     /note="This protein performs the mismatch recognition step
                     during the DNA repair process; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWI10437.1"
                     /translation="ERFITPELKTFEDKALSAQDRALAREKLLYEQLLDQLQAHIEPL
                     SALARALASVDALAALAERATTLSWCRPEFVREPCIRIEAGRHPVVAARLMETGAGNF
                     MANDCKLDAAQKMLVITGPNMGGKSTFMRQVALIALLAAMGSFVPARACRLGPIDAIH
                     TRIGAADDLANAQSTFMLEMVEAAAIIHGATEHSLVLMDEIGRGTSTFDGLALASAIA
                     SHLHDRTKAFTLFATHYFELTELPVKHERALNMHVSAAESGDDIVFLHELQPGPASRS
                     YGVQVARLAGMPASLLRQARATLEALEASKHAADAQIDLFAAPPAPVSAEPSRLDVAV
                     AALEPDMLSPKEALAALYELKNLQGKSQ"
BASE COUNT          458 a          971 c         1038 g          495 t
ORIGIN      
        1 cgtcgtcggt gacgaagact tcgccgtgct ggaacggcga catcgtgccc gggtccacgt
       61 tgaggtacgg gtcgagcttg atcagggtga ctttgaggcc gcgcgattcg aggatcgcac
      121 ccagagacgc agaagcgatg cccttgccca gcgaggacac cacaccgccg gtgacaaaga
      181 cgtacttggt cattgtgttc ggcatctgct tgacacaaat gccgtggtgg taaagcgcga
      241 ttataggtgt gtcgattcgg cgccggcccg catggattcg atcagttgca agacctcggc
      301 cgcggtctgc gccgggcgct cgagcgggaa caggtgcgag ccttcgatcg tgcggatgcg
      361 gccgcgcacc agccgttcgg tggcgcgcaa cccgaactgg cgaacctctt ccgacgcgct
      421 gccgcgcacg aacgcggccg ggcattgcag cgggtgtgcg cgcagcacgc gcgcgaggtg
      481 gtgcggcagc gtgttgtaga tcgcggtctc gatgtcgcga tgaaagctca ggcggtgcag
      541 cgtgccgtgc ggctcggtgc cgctggcgat gtagtcgcgc agcatgccgg gcgcccagcg
      601 cgcgaacatc ggcttggcgg cgaagtgctc gaacgcggcg tcggcactgt cccactgctg
      661 gcgccggcgc ttcgacacat ggccgggcga gaagcgctcg ccgatgccgg tcgccatggc
      721 gacctgcacc gcgcgcgcct tccagcccga cagcagcggc gagtcgagca tcaccagacc
      781 gctggccaga tcgggccggc gcgacgccac catcaggctc aggtagccgc cgagcgaatg
      841 gccgaccaga taggccggcc cgccggccgc gtgctcgatg aagtcgatca actgcgcgcg
      901 caggtgcggc cagttgctgg tgaccgggaa gcgcgggtcg tggccgtact tctcgatcgc
      961 gtggacctcg aagccggccg cgcgccaggc ctcgaagatc agccgatagg tgccggccgg
     1021 gaagccgttc gcatgcgaga agacgatggt cttcaaacga tcttctcgtc cggatgcgtg
     1081 atcttcttca tcggctcgta gcgcggcgac ggtacgcaca gcggcacggt cgtcggcccg
     1141 gtgccccact gcgggtcttc gagcgactcg aacacctcgc gcagcttcag gccccagttg
     1201 ccgcggatca tctgaaagta ggggttgtga tcgtcgacgc agactattct gtcggtctgc
     1261 agcgcgtgcg cggagtacac cagcaggtcc agcggcaacc cgaccgacag gttcgacttc
     1321 agggtcgagt ccatcgacac cagcgcgcat ttcgctgcct cgtcgagcgg cgtggcgggc
     1381 gtgatcatgc ggtcgagcac cggcttgccg tacttcgact cgccgacctg gaagtagcag
     1441 gtctcgcgcg tcgcctcgat gaagttgccg gccgagtaga ccaggaacag ccgcatcgcc
     1501 tcgcccttga tctggccgcc gaagatcatc gaggcgttga agtcgacgcc ggcggcggcg
     1561 agcgaggcgc cgtcctgctc gtagacgcgg cgcaccgcgg cgccgagcac gcgcaccgcg
     1621 tcgaacatgc tggtggcgtt ccagatcgtg acgggcgcgc cgtcgcggtc ctcgatcttc
     1681 tcgacctgca ggatctcgcg caccgactgg ctgatcgaca ggttgccggc cgacagcatc
     1741 accatgaagc gatcgcccgg cttctcgtac accatcatct tgcggtaggt gctgatctgg
     1801 tcgaggccgg cgttggtgcg cgagtcggac atgaacacca gtccggcgtc gagcttgatg
     1861 cctacgcagt aggtcattgc gatttccctt ggaggttctt gagttcgtac agcgccgcga
     1921 gcgcttcctt gggcgagagc atatcgggct cgagcgcggc gaccgcgacg tcgagtcgcg
     1981 atggttcggc ggacacgggc gccggcggcg cggcgaacag atcgatctgc gcgtcggccg
     2041 cgtgcttgct cgcctccagc gcctcgagcg tcgcgcgcgc ctggcgcagc aggctcgccg
     2101 gcatgcccgc caggcgcgcg acctgcacgc cgtagctgcg gctcgccggg ccgggctgca
     2161 gctcgtgcag gaacacgatg tcgtcgccgc tctcggccgc cgacacatgc atgttcagcg
     2221 cgcgctcgtg cttcaccggc agctcggtga gctcgaagta gtgggtcgcg aacagcgtga
     2281 aggccttcgt gcggtcatgc aggtggctcg cgatcgcgct ggccagcgcg aggccgtcga
     2341 aggtcgaggt gccgcgcccg atctcgtcca tcagcaccag cgagtgctcg gtcgcgccgt
     2401 ggatgatcgc ggcggcttcc accatctcga gcatgaaggt cgactgcgcg ttcgccaggt
     2461 cgtcggccgc gccgatgcgg gtgtggatcg cgtcgatcgg gcccaggcgg cacgcccgcg
     2521 cgggcacgaa cgagcccatc gcggcgagca gcgcgatcag cgcgacctgg cgcatgaagg
     2581 tgctcttgcc gcccatgttc gggccggtga tcacgagcat cttctgcgcc gcgtcgagct
     2641 tgcagtcgtt ggccatgaag ttgccggcgc cggtttccat caggcgcgcc gcgacaaccg
     2701 ggtgccggcc ggcctcgatg cggatgcagg gttcgcgcac gaactcgggc cgacaccagc
     2761 tcagcgtggt cgcgcgttcg gccagcgcgg cgagtgcgtc gacgctcgcg agcgcgcggg
     2821 ccagcgccga caacggctcg atgtgcgctt gcagctgatc aagcaattgt tcgtacagca
     2881 gcttctcgcg cgccagcgca cggtcttgcg ccgacagcgc cttgtcctcg aaggtcttca
     2941 gctccggggt gatgaagcgt tc
//
LOCUS       DATMJB010000170         2412 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_96520, whole genome shotgun sequence.
ACCESSION   DATMJB010000170 DATMJB010000000
VERSION     DATMJB010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96520"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..216
                     /gene="rpmG"
                     /locus_tag="VNU48_03835"
     CDS             49..216
                     /gene="rpmG"
                     /locus_tag="VNU48_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HWI10438.1"
                     /translation="MAKGGREKIKLESSAGTGHFYTTDKNKKTTPEKLEMIKFDPKAR
                     KHVTYKETKLK"
     gene            complement(322..1533)
                     /locus_tag="VNU48_03840"
     CDS             complement(322..1533)
                     /locus_tag="VNU48_03840"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWI10439.1"
                     /translation="MLVWGALINWLADGLVGAVWWQVLLFVLVATHITIASVTIFLHR
                     AQAHRALELHAIPSHFFRLWLWLTTGMVTKEWVAIHRKHHAKCETEDDPHSPQTRGIK
                     AVLLTGSEMYRAEAKNQETLAKFGHNTPDDWIERNLYTRYSWQGVGVMLLIDLMLFGA
                     IGAAVWALQMAWIPITAAGIINGIGHWWGYRNFEAPDASTNISPWGIIIGGEELHNNH
                     HTYPTSAKLSVKPYEFDLGWAYIRGLEMLGLAKVRKTVPLLKLGSVKPVADDKTLEAI
                     IANRYEVMAKYARDLRHASRVELVRLKAEGFASAAKLENMRLANRWLHRDEDKIPTDV
                     KARVALAVEHSPGLAKLVTMREELRSLWTRTNVSAEQLVADLQAWCKKAEESGIAALQ
                     EFALKLRAAHA"
     gene            complement(1670..>2412)
                     /locus_tag="VNU48_03845"
     CDS             complement(1670..>2412)
                     /locus_tag="VNU48_03845"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HWI10440.1"
                     /translation="ATPHSPVGLRVEGKPSLHKLDVFNDGWIEVQDEGSQLLALVTGA
                     KRGEMVVDFCAGAGGKTLALGAEMRNTGRLYAFDTSGHRLASLKPRLARSGLSNVYPV
                     QIAHERDERIKRLAGKLDRVLVDAPCSGLGTLRRNPDLKWRQTPQSVDELRVKQAAIL
                     ASASRLLKPGGRLVYATCSLLEVENEEIAQAFGAERERDFKAVPALEALTKAHVAQAD
                     TLVRGDYLRLWPHRHGTDGFFAAVWERR"
BASE COUNT          398 a          810 c          798 g          406 t
ORIGIN      
        1 aggtcgtcgc cgacctgcgc gcccgcggcg aaatctgagg agctgatcat ggcaaaaggt
       61 ggacgcgaaa agatcaagct cgagtcgagc gccggcaccg gtcatttcta cacgaccgac
      121 aagaacaaga agaccacgcc ggagaagctc gagatgatca agttcgaccc gaaggcacgc
      181 aagcacgtga cctacaagga aacgaagctg aagtaggcgc cgacgccagg cgtagaaaag
      241 ccgcccgaga ggcggctttt ttcatgcccg acaaaaggaa ggggtcgcca aggcgacccc
      301 gcgatcgtcc ttcgccgcag gctacgcgtg cgccgcgcgc agcttcagcg cgaactcctg
      361 cagcgcggcg atgccgctct cctcggcctt cttgcaccag gcctgcaggt cggcgacgag
      421 ctgctcggcc gagacgttgg tgcgcgtcca tagcgagcgc agttcctcgc gcatcgtcac
      481 cagcttggcc aggcccgggc tgtgttcgac ggccagcgcc acgcgcgcct tcacgtcggt
      541 ggggatcttg tcctcgtcgc ggtgcagcca gcggttcgcc agccgcatgt tctcgagctt
      601 ggccgcgctg gcgaagccct ccgccttcag gcgcacgagc tcgacacggc tggcatggcg
      661 caggtcgcgc gcgtacttgg ccatgacctc gtagcggttc gcgatgatcg cctcgagcgt
      721 cttgtcgtcg gcaaccggct tgacgctgcc gagcttcagc agcggcacgg tcttgcgcac
      781 cttggccagg cccagcatct cgaggccgcg gatgtaagcc cagccgaggt cgaactcgta
      841 gggcttgacc gacagcttgg ccgaggtcgg gtaggtgtgg tggttgttgt gcagctcctc
      901 gccgccgatg atgatgcccc acggcgaaat gttcgtcgac gcgtcgggcg cctcgaaatt
      961 tcggtagccc caccagtggc cgatgccgtt gatgatgccg gcggcggtga tcggaatcca
     1021 cgccatctgc agcgcccaca cggcggcgcc gatcgcgccg aacagcatca ggtcgatcag
     1081 cagcatcacg cccacgccct gccagctgta gcgggtgtac aggttgcgtt cgatccagtc
     1141 gtccggcgtg ttgtggccga acttggcgag cgtttcctgg ttcttcgcct cggcgcggta
     1201 catctcgctg ccggtcagca gcaccgcctt gatgccgcgc gtctgcgggc tgtgcgggtc
     1261 gtcttcggtc tcgcacttgg cgtggtgctt gcggtggatc gcgacccatt ccttggtcac
     1321 catgccggtg gtcagccaca gccagaggcg gaagaagtgc gacggaatcg cgtgcagttc
     1381 gagcgcgcgg tgcgcctgcg cgcggtgcag gaagatcgtc accgaagcga tcgtgatgtg
     1441 cgtggccacc agcacaaaca gcagcacctg ccaccacacg gcgccgacca ggccatcggc
     1501 cagccagttg atcagcgcac cccaaacaag catcaacgaa tccatttgta cctttagcct
     1561 gcttgacgcg caggtgggca attgtaaagg actgcccccg ggcaatcccc ttcgatttga
     1621 cgggtatgcg ggcccctgtt tgccctatgt caaggcaggc ccgtgaaact caacggcgct
     1681 cccacaccgc cgcgaagaag ccgtcggtgc cgtggcgatg cggccacagc cgcaggtagt
     1741 cgccgcggac cagcgtgtcg gcctgcgcga cgtgggcctt ggtgagcgcc tcgagcgccg
     1801 gcacggcctt gaagtcgcgc tcgcgctcgg cgccgaaggc ttgcgcgatc tcctcgttct
     1861 cgacctcgag caggctgcag gtcgcataga ccagtcgacc gccgggcttg agcaggcgcg
     1921 acgcgctcgc caggatcgcc gcctgcttga cgcgcaactc gtcgaccgac tgcggcgtct
     1981 ggcgccattt caggtccggg ttgcggcgca gcgtgcccag gcccgagcac ggcgcgtcga
     2041 ccagcacgcg gtcgagcttg ccggccagcc gcttaatgcg ctcgtcgcgc tcgtgcgcga
     2101 tctgcaccgg gtagacgttc gacaggccgc tgcgcgccag acgcggcttc agcgacgcga
     2161 gccggtggcc cgaggtgtcg aatgcataga gccgcccggt gttgcgcatc tcggcgccga
     2221 gcgcgagcgt cttgccgccg gcgccggcgc agaagtcgac caccatctcg ccgcgtttcg
     2281 cgccggtgac gagggcgagc agctggctgc cttcgtcctg cacctcgatc cagccgtcgt
     2341 tgaagacgtc gagcttatgc agcgagggct tgccttcgac ccgcaacccg accggcgaat
     2401 gcggcgtcgc ct
//
LOCUS       DATMJB010000171         2008 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_156055, whole genome shotgun sequence.
ACCESSION   DATMJB010000171 DATMJB010000000
VERSION     DATMJB010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156055"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1745
                     /locus_tag="VNU48_03850"
     CDS             132..1745
                     /locus_tag="VNU48_03850"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HWI10441.1"
                     /translation="MTKRLLPLIPAGLTVVQVLSTPDIITIVASSDASTAACPSCGRL
                     SRCVHSTYQRRLQDLPWQGRAVVIQVRARRFRCPDPACARRTFVEPLCGTAVRRAWRT
                     CRLGDLQRHIGLAAGGEAGSRLARRLAMPVSPDTLLRLVARALVSTTSAPKVLAVDEW
                     AWRKGRRYGTVLVDLERNRVVDLLPDRQAQTLATWLRAHPGIEVVARDRAGAFADGAA
                     QGAPEAVQVADRWHLLRNVGDALRHAVERHHAALRRIGREIAAELATERATDAPTLRP
                     EPVPTALERRRTEARARRQALYDEAVRLRQGGATLKSIAVALGTPSRTLAYWFATGHA
                     PLCDRRPSGSILDPFRGYLEQRYAEGCRNARQLWRELREQDFQGRPSIVRVWIGRWNK
                     AASGGVPARGSSGPGWKLPSVHAVTRLLTSPRDGAPDGDQRLCARLLEEVPELAAAAD
                     AARRLGRVLRKESAEPLIDVLTATKQTLLNRLAISLEGDAAAVQAALDTPWTTSPAEG
                     QINKLKTLKRAMYGRAGFPLLRARVLQVA"
BASE COUNT          322 a          694 c          673 g          319 t
ORIGIN      
        1 tgtagccgca atatagagat gacacccctc tgcaaagttg aggctctccc gcacgaagcg
       61 tgctgggcta ggcgggaggg ccgggcgtga gcacatagga ggaacatttt actcttcacc
      121 aagcaggttc tttgaccaaa cgccttctgc ctctgatccc agcaggtctc accgtcgttc
      181 aggttctatc cacacccgac atcatcacca tcgtcgccag ctcagacgcg agcacggccg
      241 catgcccgag ctgtggccgc ctgtcgcggt gcgtgcacag cacctaccag cgccggctgc
      301 aggacctgcc gtggcagggc cgtgccgtcg tgatccaagt tcgcgcgcgg cggttccgct
      361 gcccagaccc ggcctgtgcc cgtcggacct tcgtcgagcc gctgtgcgga accgctgtgc
      421 gccgggcttg gcggacctgc cggctcggcg atctgcagcg gcacatcggc ctggccgcgg
      481 gtggagaggc cggctcgcgc ctggcccggc gcttggcgat gccggtgagc ccggacacgc
      541 tgctcaggct ggttgcgcgc gctctcgtgt cgacgacgtc ggcgcccaaa gtgctcgctg
      601 tcgatgagtg ggcctggcgc aagggacgcc gctacggcac ggtgctggtc gacctcgagc
      661 gcaaccgggt cgtggacctg ctacctgacc ggcaggccca gacgctcgcg acctggctgc
      721 gcgcgcatcc cggtatcgag gtcgtcgcgc gcgatcgcgc cggcgccttc gcggacggcg
      781 ctgcccaggg cgcacccgaa gcggtccagg tcgccgaccg ctggcacctg ctgcgcaacg
      841 tcggcgacgc gctgcgccac gcggttgagc gccaccatgc cgccttacgc cggatcggcc
      901 gcgagatcgc cgcggaactc gccaccgagc gcgccacgga cgcccccaca ctcaggccgg
      961 agccggtgcc cacggccctg gaacgccgcc gaaccgaggc gcgggcacgg cggcaggcgc
     1021 tctacgacga ggcggtgcgc ttgcgtcagg gcggcgcgac gctcaagtcc attgccgtgg
     1081 cactcggcac gccgtcacgc acgctcgcat actggtttgc gaccggtcat gcaccgctgt
     1141 gcgaccgcag gccgtcgggc agcatcctcg acccgttccg gggctacctt gagcagcgct
     1201 atgccgaggg ctgccgcaac gcgcgccagc tctggcgcga actgcgtgag caagacttcc
     1261 aaggtcggcc cagtatcgtg cgggtctgga tcggacgctg gaacaaggca gcgtcaggcg
     1321 gcgtccctgc tcgcgggtca tcgggcccgg gctggaagct gccctcagtt cacgccgtca
     1381 cgcgccttct gacgtctcct cgcgacggtg cgccggacgg cgaccagcgg ctgtgcgcgc
     1441 gattgctgga ggaggtgcca gagcttgctg ccgcggccga tgccgcgcgc cggctcggcc
     1501 gggtgttgcg caaggagagc gccgagccgc tcatcgatgt gctgaccgca acgaagcaga
     1561 cgctcctgaa tcggctggcc atcagcctgg aaggcgatgc agcggccgtg caggccgcac
     1621 tcgacacgcc gtggacgact agtcctgccg aaggccagat caataagctg aagacgctga
     1681 agcgggcgat gtatggccgc gctggtttcc cactcctgcg cgcacgcgtc ctacaagtcg
     1741 catgataccc gttgtccttg gtcctgaccc attcaacgcc tgcgtcgaag gcgcataagg
     1801 cggcactccg ccccgcagta gcaggtcatt tttgaggata accggtatga caccagacaa
     1861 cagcacgctt cgtgcgggag agcccctatc tggcagagga gtgacatctc tattcggcgg
     1921 agacaccgct atgcgcggct ctcgacggtg agacacaaaa tgttgtgata cggcccgtga
     1981 tcgcattgca cctgagtgcc aaggcatc
//
LOCUS       DATMJB010000172         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_757413, whole genome shotgun sequence.
ACCESSION   DATMJB010000172 DATMJB010000000
VERSION     DATMJB010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757413"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1977)
                     /gene="thrS"
                     /locus_tag="VNU48_03855"
     CDS             complement(58..1977)
                     /gene="thrS"
                     /locus_tag="VNU48_03855"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWI10442.1"
                     /translation="MISIQLPDGSKRDYPGPVTVAEVAASIGTGLAKAALAGRVGQGD
                     QARVVDTSFLIDHDTPLAIVTDKDAAGLEVIRHSTAHLLAYAVKELFPDAQVTIGPVI
                     ENGFFYDFSYKRPFTPEDLAAIEAKMTELSRRDEKVERRVLPRDAAVAYFKSLGEHYK
                     AEIIASIPAGEDVSLYREGAFEDLCRGPHVPSTGKLKHFKLMKVAGAYWRGDHRNEML
                     QRIYGTAWATKDELQQHLHMLEEAEKRDHRKLGRELDLFHLDEFAPGLVFWHPKGWAL
                     WQQVEQYMRQVYRDCGYLEVKGPQILDKSLWEKTGHWDNYRDNMFTTESEKREYALKP
                     MNCPGHVLIFKSHMRSYRELPIRYGEFGQCHRNEPSGAMHGIMRVRGFTQDDGHIFCT
                     EDHILEECVAYTTQLQAVYRDFGFTEIIYKVATRPEPRIGSDALWDKAEQALMESLRR
                     SGCEFAIAPGEGAFYGPKIEYTLKDALGRHWQCGTMQVDFNMAERLGGEYVTESSGRA
                     HPVMLHRAIIGSFERFIGMLIEHHAGALPAWLAPLQVSVLNISEGQSDYAREVVKTLQ
                     KQGLRVVSDLRNEKITYKIREHSLQKVPYILVVGDKEKANGAVAVRARGNQDLGVMPL
                     ADFAEKIAADIAQRT"
BASE COUNT          331 a          682 c          656 g          395 t
ORIGIN      
        1 ccggttcaca ggcttgcaac gccaccgaag tggcgtcgtc gcgttttcaa gcgcggttca
       61 agtcctttgg gcgatgtcag cggcgatctt ctcagcgaag tcggccagtg gcatcacacc
      121 gaggtcctgg ttgccccggg cgcgcaccgc aacagccccg tttgccttct ccttgtcacc
      181 cacgacaagg atgtacggaa ccttttgcaa ggaatgctcg cggattttat aggttatttt
      241 ctcgttgcgc aaatcggaca ccaccctaag tccttgtttt tgcagcgttt tgaccacttc
      301 ccgggcgtag tcactctggc cctcgctgat gttgagcacg ctgacctgca acggcgccag
      361 ccaggcgggc agcgcgccgg cgtggtgttc gatcagcatg ccgatgaaac gctcgaagct
      421 gccgatgatc gcccggtgca gcatcaccgg gtgggcgcgg ccgctggact cggtcacgta
      481 ctcgccgccc agacgctcgg ccatgttgaa gtcgacctgc atcgtgccgc actgccagtg
      541 gcggccgagc gcatccttca gcgtgtactc gatcttcggg ccgtagaacg cgccctcgcc
      601 gggcgcgatc gcgaactcgc agccggagcg gcgcagcgac tccatcagcg cctgctcggc
      661 cttgtcccac agcgcgtcgg aaccgatgcg cggctcgggc cttgtggcga ccttgtagat
      721 gatctcggtg aagccgaagt cgcgatagac cgcctgcagc tgcgtcgtgt aggccacgca
      781 ctcctcgagg atgtggtcct cggtgcagaa gatgtggccg tcgtcctgcg taaagccgcg
      841 cacccgcatg atgccgtgca tcgcgccgct gggttcgttg cggtggcact ggccgaactc
      901 gccgtagcgg atcggcagct cgcggtagct gcgcatgtgc gacttgaaga tcagcacgtg
      961 gcccgggcag ttcatcggct tgagcgcgta ctcgcgcttt tccgactcgg tcgtgaacat
     1021 gttgtcgcgg tagttgtccc agtggccggt cttctcccac aggctcttgt ccaggatctg
     1081 cgggcccttg acctcgaggt agccgcagtc gcggtagacc tgccgcatgt actgctcgac
     1141 ctgctgccac agcgcccagc ccttcgggtg ccagaacacc aggcccggcg cgaactcgtc
     1201 gaggtggaac aggtcgagct cgcgcccgag cttgcggtgg tcgcgcttct cggcctcctc
     1261 gagcatgtgc aggtgctgct gcagctcgtc cttggtcgcc caggccgtgc cgtagatgcg
     1321 ctgcagcatc tcgttgcgat gatcgccgcg ccagtaggcg ccggccacct tcatcagctt
     1381 gaagtgtttc aacttgccgg tgctgggcac gtgcgggccg cggcacaggt cctcgaaggc
     1441 gccttcgcgg tacagcgaca catcttcacc agccggaatg ctcgcgatga tctcggcctt
     1501 gtagtgctcg cccaggctct tgaagtacgc caccgccgcg tcgcgtggca gcacgcgacg
     1561 ctcgaccttc tcatcgcgcc tgctcagctc ggtcatcttc gcctcgatcg ccgccaggtc
     1621 ctcgggcgtg aacggacgct tgtacgagaa gtcgtagaag aagccgttct cgatcaccgg
     1681 gccgatcgtg acctgggcgt cggggaacag ctccttcact gcataagcga gcaggtgcgc
     1741 cgtcgagtgg cggatcacct cgaggccagc ggcgtccttg tcggtgacga tcgccagcgg
     1801 cgtgtcgtga tcgatcagga agctcgtgtc gaccacccgg gcctggtcgc cctgccccac
     1861 gcgccccgcg agcgccgcct tggccaggcc ggtgccgatg gacgccgcca cctcggccac
     1921 cgtcaccggg ccggggtagt cgcgtttcga gccgtcgggc aactgaatcg agatcatcga
     1981 agactccaaa agcaaaaagg cgcggaccga gccgcgcctt gtatcaacga aaagggaaat
     2041 gaacgggcag gtcgagacgc ggcc
//
LOCUS       DATMJB010000173         2091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_748076, whole genome shotgun sequence.
ACCESSION   DATMJB010000173 DATMJB010000000
VERSION     DATMJB010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748076"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /gene="dnaE"
                     /locus_tag="VNU48_03860"
     CDS             complement(<1..1425)
                     /gene="dnaE"
                     /locus_tag="VNU48_03860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWI10443.1"
                     /translation="MAFVHLRTHTEFSIADGTLRIDDAVACAAADGQAALAITDLSNL
                     FGAVKLYGAARKKGVKPIIGADLWLEPEGGEKAPSRLVVLVQDKIGYRNLCELLSRAW
                     LQHEQRSQACVKWAWLAELNTGLIALAGAEMGAVGQALVAGDPMRADALAQRLAAIFA
                     NRFYIELQRAGLPGNEAHVRAAAQLAARLQLPVVATHPVQFASADDFDAHEARVCVAE
                     GEMLTNPRRVKRFSREQYFKTQAQMQALFADLPSAIANTTEIAKRCSLSLVLGKPQLP
                     DFPTPLVDGRPLAMAEYFSILSHQGLVERLKQRYPIEAEREQQRPRYVERLDFEIATI
                     LKMGFPGYFLIVADFIRWAQHNGCPVGPGRGSGAGSLVAYSLNITGLDPLQYNLLFER
                     FLNPERVSMPDFDIDFCQGNRDRVIDYVKQKYGRDAVSQIATFGTMAAKAALRDVGRV
                     LGMGYGHVDSIAKLIPAPPGKSVTL"
     gene            1467..>2091
                     /locus_tag="VNU48_03865"
     CDS             1467..>2091
                     /locus_tag="VNU48_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWI10444.1"
                     /translation="MKSILNISAYKFVGLRDTAQWRESIRLQAQAGALKGTVLLADEG
                     INLVLAGAPAGVRGFLDWLSAHAAFADLAVKQSWSDTVPFGRLLVKVKAEIIRMNHPT
                     IRPHAARAPAVDAPTLARWLDAGVDDAGRELVTLDTRNAFEVDQGRFRGAHDWRLRRF
                     SDFPQALREHRAQLEGKTVVSYCTGGIRCEKAALLMREAGLANVLQLD"
BASE COUNT          322 a          706 c          732 g          331 t
ORIGIN      
        1 gagcgtgacc gacttgccgg gcggcgccgg gatcagcttg gcgatggaat cgacgtggcc
       61 gtagcccatg cccagcacgc ggccgacgtc gcgcagcgcg gccttggccg ccatcgtgcc
      121 gaaggtggcg atctggctga ccgcatcgcg cccgtatttt tgcttgacat agtcaatcac
      181 ccggtcgcga ttgccctggc agaagtcgat gtcgaagtcg ggcatcgaca cgcgttccgg
      241 gttcaggaag cgctcgaaca gcaggttgta ctgcagcggg tccagcccgg tgatgttgag
      301 cgaatacgcg accagcgacc cggcccccga gccgcgcccc ggcccgaccg ggcagccgtt
      361 gtgctgcgcc cagcggatga agtcggcgac gatcaggaag tagcccggaa agcccatctt
      421 caggatcgtc gcgatctcga agtcgagccg ctcgacgtag cgcgggcgct gctgctcgcg
      481 ctcggcttca atcggatagc gctgcttcag ccgctcgacc aggccctggt gcgacaggat
      541 gctgaagtac tctgccatcg cgagcggccg gccgtcgacg agcggcgtcg ggaagtccgg
      601 caactgtggt ttgccgagca ccaggctgag gctgcagcgc ttcgcgatct cggtggtgtt
      661 cgcgatcgcg ctcggcaggt cggcgaacag ggcctgcatc tgcgcctggg tcttgaagta
      721 ctgctcgcgg ctgaagcgct tgacgcgcct cgggttggtg agcatctcgc cctcggcgac
      781 gcacacgcgc gcctcgtgcg cgtcgaaatc gtcggcgctg gcgaactgca ccgggtgcgt
      841 cgcgaccacc ggcagttgca gccgcgccgc gagttgcgcc gcggcgcgca catgcgcctc
      901 gttgccgggc aggccagcgc gctgcaactc gatgtagaag cggttcgcga agatcgccgc
      961 gagccgctgc gccagcgcgt cggcgcgcat gggatcgccg gcgaccagcg cctggccgac
     1021 cgcgcccatc tcggcgccag ccagcgcgat caggcccgtg ttcagctccg cgagccaggc
     1081 ccatttgacg caggcctgcg aacgctgctc gtgctgcagc caggcgcgcg acagcagctc
     1141 gcacaggttg cgataaccga tcttgtcttg caccagcacg acgagccggc tgggcgcctt
     1201 ctcgccgcct tcgggctcga gccagaggtc ggcgccgatg atcggcttga cgcccttctt
     1261 gcgcgccgcg ccgtacagct tgaccgcgcc gaacaggttc gacaggtcgg tgatcgcgag
     1321 cgccgcctgg ccgtccgctg ccgcgcacgc caccgcgtcg tcgatccgca aggtgccgtc
     1381 ggcgatggaa aactcggtgt gggtgcgcag gtgaacgaaa gccatgcggt cattttaggg
     1441 agcgcgcgcc tcgtaaaatt tgcgtggtga aatcaattct gaacatctcg gcctacaagt
     1501 tcgtgggtct gcgcgacacg gcgcagtggc gcgaaagcat ccgcttacag gcgcaggccg
     1561 gcgcgctcaa gggcacggtg ctgctggccg acgagggcat caacctggtc ctcgccggcg
     1621 caccggccgg cgttcgcggc tttctcgact ggctgagcgc gcacgcggcg tttgccgatc
     1681 tggcggtgaa gcagagctgg tccgacacgg tgccgttcgg ccggctgctg gtcaaggtca
     1741 aggccgagat catccgcatg aaccacccga cgatccggcc gcacgccgcg cgcgccccgg
     1801 cggtcgacgc gccgacgctc gcgcgctggc tggacgccgg cgtcgacgac gcgggccgcg
     1861 agctcgtcac gctcgacacg cgcaatgcgt tcgaggtcga tcagggccgc ttccgcggtg
     1921 cacacgattg gcggctgcgc aggttcagcg actttccgca ggcgctgcgc gagcaccgcg
     1981 cccagctcga gggcaagacc gtggtgagct actgcaccgg cggcatccgc tgcgaaaagg
     2041 cggcgttgct gatgcgcgag gcgggcctgg caaacgtgct gcagctcgac g
//
LOCUS       DATMJB010000174         3716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_333172, whole genome shotgun sequence.
ACCESSION   DATMJB010000174 DATMJB010000000
VERSION     DATMJB010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333172"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /gene="tilS"
                     /locus_tag="VNU48_03870"
     CDS             <1..1028
                     /gene="tilS"
                     /locus_tag="VNU48_03870"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWI10445.1"
                     /translation="TALARMAREAGASAILLAHHRRDQAETVLLQALRGAGVAGLAGM
                     PRSVQRDGITWLRPWLDRPRAEIEAYVRRHRLAYVDDASNADPRFARNRLRLQVWPVL
                     EGAFPQAETALASTAEWAQQASALLTEIAALDLAQIGSTQGPLDVRAWSTLSAARRGN
                     ALRLWLQQAHGAAAPASLVVRLLGELPTARNARWPLGDAELRLYRGRLGLVPNAVAAP
                     VAVPRAATLSVRRAGRCVLAGWQGALQIERVEQGGVPLAWLAQLELRPRAGGEQFQAG
                     AGRPPRSLKKQYQEAGVPTWERGGPLIYSGGQLVFVPGLGLDARVIGLPGQPLVTLRW
                     LPLVGKG"
     gene            1131..2399
                     /locus_tag="VNU48_03875"
     CDS             1131..2399
                     /locus_tag="VNU48_03875"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWI10446.1"
                     /translation="MALTVHKYGGTSMGSPDRIRSVAKRVAKWARAGHQMVVVPSAMS
                     GETNRLLGLAKELSPERVTPELQRELDMIASTGEQVSVGLLAIALQAEGLASVSYAGW
                     QVPIRTDSAYTKARIQSIDDQRVRADLAAGKVVIITGFQGVDEHGHITTLGRGGSDTS
                     AVAVAAAMKADECLIYTDVDGVYTTDPRIVADARRLKTISFEEMLEMASLGSKVLQIR
                     SVEFAGKYRVPLRVLSSFTPWDIPIDEEAQSGTLITFEEDEKMEQAVVSGIAFNRDEA
                     KISLLGVTDKPGIAYLILGPVAEANIDVDVIIQNVSHDGRTDFSFTVHRNDYARTMDL
                     LKAKVLPVTGATDLIGDAKICKVSIVGIGMRSHVGVASRMFSVLSQEGINIQMITTSE
                     IKTSVVIDEKYMELAVRALHKASDLEQAAA"
     gene            2438..2530
                     /locus_tag="VNU48_03880"
     tRNA            2438..2530
                     /locus_tag="VNU48_03880"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2472..2474,aa:Ser,seq:gct)
     gene            2652..>3716
                     /locus_tag="VNU48_03885"
     CDS             2652..>3716
                     /locus_tag="VNU48_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWI10447.1"
                     /translation="MQYLWHRVRHQALAWAALIACAPALHAQAAPVTNLPSVEVVSTS
                     PLPGTGVPRDQVPSNVQTADGKRLRQLQSLNLPEFMASQLASVNINETQGNPFQMDLN
                     YRGFTASPLLGTPQGLSVYLDGVRVNEPFGDVVNWDMIPSTALASVTLLPGSNPLFGL
                     NTLGGALALKTKSGDTYPGGELEVRGGSFGRLSTEFSDGRSIGEHGHLFAALGSFDED
                     GWRDASPSRVRQLFLKGGERSVDHSWDLSLLHGWSRLVGNGPLPEDMLAQRRSQVYTL
                     PDPTDNRVTMLTLNATREFAHGIELATTLYTRRVNAATVNGDLNDAYDPPAVPESAIE
                     HRTRTRERGQGLALQLSQTLG"
BASE COUNT          593 a         1286 c         1291 g          546 t
ORIGIN      
        1 ggaccgcgtt ggcgcgcatg gcgcgcgagg ccggcgcgag cgcgattctg ctggcgcatc
       61 accgccgcga ccaggccgag accgtgctgc tgcaggcgct gcgcggtgcc ggcgtggccg
      121 gtctggcggg catgccgcgc agcgtgcaac gcgacggcat cacctggctg cggccctggc
      181 tggaccggcc gcgggccgag atcgaggcct acgtgcgccg ccatcgcctg gcctacgtcg
      241 acgacgccag caatgccgat ccgcgcttcg cgcgcaaccg gttgcgcctg caggtctggc
      301 cggtactgga aggcgcgttc ccgcaagcgg aaacggcgct cgcgagcacg gccgagtggg
      361 cacagcaggc gagcgcgctg ctcaccgaga tcgctgcgct ggacctggcg cagatcggca
      421 gcactcaggg gccgctggat gtccgggcct ggtcgacgct gtcggccgcg cgccgcggca
      481 atgcattgcg cctctggttg cagcaagcgc atggagcggc tgcaccggcg agcctggtcg
      541 tgcgattgct tggcgaactg ccgacggcgc gcaacgcacg ctggccgctc ggcgacgccg
      601 aactgcggct gtaccgcggt cgcctcggcc tggtgccgaa tgcggtcgcg gcgcccgtcg
      661 ctgttccgcg cgccgccacg ctcagcgtgc gtcgcgccgg ccgctgcgtg ctggccggct
      721 ggcagggcgc gctccagatc gagcgcgtcg agcagggcgg cgtgccgctg gcgtggctgg
      781 cgcaactcga actgcgccca cgtgccggcg gcgagcagtt ccaggccggt gccggccggc
      841 cgccgcgcag cctgaagaag cagtaccagg aggccggcgt gccgacgtgg gagcgcggcg
      901 gcccgctgat ctacagcggc ggccagctcg tgttcgtgcc cggccttggg ctggacgcgc
      961 gcgtgatcgg cctgccgggc cagccgctcg tgacgctgcg ctggctgccg ctcgtcggca
     1021 agggctgagg tgctcggtcg ccaacgttag aatcgcgggc tcgcccgggc gcgatacgcc
     1081 cttccaccag cgctgcgggc tcgtctttcg ttcgtgacct gactcactcc atggccctta
     1141 cagtccacaa gtacggcggc acctcgatgg gttcgccgga tcgaatccgc agcgttgcca
     1201 agcgcgtcgc caaatgggcc cgcgccggcc accagatggt ggtcgtgccg tcggcgatga
     1261 gtggcgagac gaatcgcctg ctcgggctgg cgaaggagct ttcgccggaa cgcgtcacgc
     1321 ccgaactgca gcgcgaactc gacatgatcg cctccaccgg cgagcaggtc tcggtcgggc
     1381 tgctggcgat cgcgctgcag gccgaagggc tggcgtcggt cagctatgcc ggctggcagg
     1441 tgccgatccg caccgattcc gcctacacca aggcgcgcat ccagagcatc gacgaccagc
     1501 gcgtgcgcgc cgacctcgcg gccggcaagg tcgtgatcat caccggcttc cagggcgtcg
     1561 acgagcacgg ccacatcacg accttggggc ggggcggctc cgacacgtcg gcggtggcgg
     1621 tggcggcggc gatgaaggcc gacgagtgcc tgatctacac cgacgtcgac ggcgtctaca
     1681 ccaccgatcc gcgcatcgtc gccgacgcgc ggcgcctgaa gacgatcagc ttcgaagaga
     1741 tgctcgagat ggcgagcctg ggttcgaagg tgctgcagat ccgctcggtc gaattcgccg
     1801 gcaagtaccg cgtgccgctg cgcgtgctgt cgagcttcac gccctgggac attccgatcg
     1861 acgaagaggc gcaatcgggt acgttgatca cattcgagga agacgaaaaa atggaacaag
     1921 ccgttgtctc gggcatcgcg ttcaatcgcg acgaagccaa gatcagcctg ctgggcgtga
     1981 ccgataagcc gggcatcgca tacctgatcc tcgggccggt ggccgaggcc aacatcgatg
     2041 tcgacgtgat catccagaac gtctcgcacg acggccgcac cgacttctcg ttcaccgtgc
     2101 atcgcaacga ctacgcgcgc acgatggacc tgctgaaggc gaaggtgctg ccggtgaccg
     2161 gcgcgaccga cctgatcggc gacgcgaaga tctgcaaggt cagcatcgtg ggcatcggca
     2221 tgcgctcgca cgtcggcgtg gccagccgca tgttcagcgt gctgagccag gagggcatca
     2281 acatccagat gatcacgacc agcgagatca agaccagcgt cgtgatcgac gagaagtaca
     2341 tggaactcgc ggtgcgcgcg ctgcacaagg cctccgacct ggagcaggcc gcggcctgag
     2401 cgcgtgctgc ttcgcggtta gaatgcgcgc tgttcacgga gctgtgaccg agtggccgaa
     2461 ggtgctcccc tgctaaggga gtaagcgggc aaaacctgct tcgagggttc gaatccctcc
     2521 ggctccgcca gagaacaagc aaatacgggc cttggcggcc cgttttgcgt ttctacctcg
     2581 tcgctgctcg cgctgctgcc acgcctttct ttgatagctt cggcgcagac cagcagactc
     2641 aggagactcg aatgcagtac ctgtggcacc gcgtgcgcca ccaagcgctg gcgtgggccg
     2701 cgctgatcgc ctgcgcgccg gcgctgcacg cgcaagccgc gccggtcacg aacttgccgt
     2761 cggtcgaggt cgtcagcacc tcgccgctgc ccggcaccgg cgtgcccagg gaccaggtgc
     2821 cgtcgaatgt gcagaccgcc gacggcaagc gtctgcgtca gctgcagagc ctgaacctgc
     2881 ccgagttcat ggccagccag ctcgccagcg tcaacatcaa cgagacgcag ggcaacccgt
     2941 tccagatgga cctgaactac cgcggcttca ccgccagccc gctgctgggc acgccgcagg
     3001 gcctgtcggt gtacctcgac ggcgtgcgcg tcaacgagcc cttcggcgac gtcgtcaact
     3061 gggacatgat cccgagcacc gcgctcgcca gcgtgacgct gctgcccggc tcgaacccgc
     3121 tgttcggcct gaacacgctc ggcggcgcgc tggcgctgaa gaccaagagc ggcgacacct
     3181 acccgggcgg tgagctcgag gtgcgcggcg gctcgttcgg ccgcctgagc accgagttca
     3241 gcgacgggcg ctcgatcggc gagcacggcc acctgttcgc cgcgctgggc agcttcgatg
     3301 aagacggctg gcgcgacgcc tcaccgtcgc gggtgcgtca gctgttcctg aagggcggcg
     3361 aacgcagcgt cgaccattcg tgggacctga gcctgctgca cggctggagc cgactggtcg
     3421 gcaacggccc gctgcccgag gacatgctgg cgcagcgccg ctcgcaggtc tacacgctcc
     3481 cggacccgac cgacaaccgg gtgacgatgc tgaccctgaa cgcgacgcgc gagttcgcgc
     3541 acgggatcga gctggccacc acgctctaca cccgccgcgt caacgcggcc accgtcaacg
     3601 gcgacctcaa cgatgcctac gacccgccgg ccgtgcccga gtcggcgatc gagcaccgca
     3661 cccgcacgcg cgagcgcggc cagggcctgg ccctgcagct gagccagacg ctgggg
//
LOCUS       DATMJB010000175         4823 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_510905, whole genome shotgun sequence.
ACCESSION   DATMJB010000175 DATMJB010000000
VERSION     DATMJB010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510905"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VNU48_03890"
     CDS             <1..734
                     /locus_tag="VNU48_03890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWI10448.1"
                     /translation="GGDVSGVLNGVDGAVWDPSNDAGIAMRYSATELGGKARCKATLQ
                     AELGLTVRADAPLFGVVSRLTSQKGLDLVLGALPGLLKRGGQLALQGAGDAALEAAFV
                     AAAKAHPGEVAVRIGYDEAFAHRLIAGADAILVPSRFEPCGLTQLYGLRYGTLPVVRR
                     TGGLADTVVDANDEALQADRATGFAFDAATPAALDAALQRAGTLYAQPERWRRLMARA
                     MAQDFSWGGAAQKYIALYRDLIRPK"
     gene            795..1052
                     /locus_tag="VNU48_03895"
     CDS             795..1052
                     /locus_tag="VNU48_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10449.1"
                     /translation="MRTIRLSDPFPRGKPAKKLEWGSSAWAADSCLVEWSRFEREWLE
                     PTPIKRAAPEPVWKWSTSALPLNVWLGLGLLGAALIWVTWG"
     gene            1311..2318
                     /gene="bioB"
                     /locus_tag="VNU48_03900"
     CDS             1311..2318
                     /gene="bioB"
                     /locus_tag="VNU48_03900"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HWI10450.1"
                     /translation="MTTTIATIPLSAVRATASRVCAPAATSWRVADIEALFDLPFMDL
                     LFRAQQAHREQFDASEVQLSTLLSIKTGGCPEDCGYCPQSAHFDTGVEASKLMPLQEV
                     VDAAQAAKAQGATRFCMGAAWRSPKARDIERVSEMVREVKALGLETCMTLGMLDADQA
                     QALDAAGLDYYNHNLDSAPEFYGQIIGTRTYQDRLDTLAHVRQAGIKVCSGGIVGMGE
                     SRAQRAGLIAQLANLDPYPESVPINNLVQVEGTPLAGTAPLDPFEFVRTIAVARITMP
                     KTMVRLSAGREQMDDALQALCFAAGANSIFYGDRLLTTANPQAARDRALFERLGLRAM
                     T"
     gene            2315..3124
                     /gene="panB"
                     /locus_tag="VNU48_03905"
     CDS             2315..3124
                     /gene="panB"
                     /locus_tag="VNU48_03905"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWI10451.1"
                     /translation="MSDVRKPMTLHRLRAMHTQGEKIAMLTCYDASFARLLDAAGVDV
                     LLVGDSLGMVLQGHDSTVPVSLDEMAYHTQCVARGNAHAWIIGDLPFGSYHASFDQAM
                     HGAARLMQSGAHMVKLEGGGWTAETVRLLVERGIPVCAHLGLTPQSVHALGGYRVQGR
                     DEQAAQTLRRHARELAEAGASMLVLELMPSALAGELTAALPIPVIGIGAGAACSGQVL
                     VLHDMLGITQGTLPRFVRDFMAGSAGIEQALRRYVAAVKDGSFPVESIHTY"
     gene            3148..3984
                     /gene="panC"
                     /locus_tag="VNU48_03910"
     CDS             3148..3984
                     /gene="panC"
                     /locus_tag="VNU48_03910"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HWI10452.1"
                     /translation="MRVIHTIADLRQALAGIDGVAFVPTMGNLHAGHLSLIERAKQAG
                     GPVVASIFVNRLQFAPHEDFDTYPRTFERDCELLRGAGCNFVFAPDEAELYPEPQAYT
                     VQPPAALADILEGHFRPGFFTGVCTVVLKLFNCVQPRVAVFGKKDYQQLMVISRMVRQ
                     LALPIEIVAGETTRAEDGLALSSRNGYLDAAQRAEAVTLSRILKAIADDVRRGRRDWP
                     ALEDTAMRSLTARGWQPDYVAVRRRADLALPTTGDALVVVAAARLGSTRLIDNLELDV
                     PA"
     gene            4104..>4823
                     /locus_tag="VNU48_03915"
     CDS             4104..>4823
                     /locus_tag="VNU48_03915"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWI10453.1"
                     /translation="MAQLIEHLADLTGYRDRDQLDVTVVRAIKDVLRPRCVSIYRCIG
                     EPGAQRWLTRARMGPGDATAMADPLSAEISALPHLDDVPARRDCVTRAEVVTVGAGPQ
                     LNDFPIATEREVIGVLELETAEALTASDRAMIRTILRIYGNFHDLLDHSERDPLTGLL
                     NRQTFDAAFMTRAASSHPGDEAAVSGRRAAAVSESAVWLGVVDIDHFKHVNDTYGHLI
                     GDEVLLLLSRLLRSTFRHHDRL"
BASE COUNT          727 a         1709 c         1677 g          710 t
ORIGIN      
        1 gcggcggcga cgtgagcggt gtgctcaatg gcgtggacgg cgcggtctgg gatccgtcga
       61 acgatgccgg catcgccatg cgctacagcg cgaccgaact cggcggcaag gcgcgctgca
      121 aggcgacgct gcaggccgaa ctcgggttga ccgtgcgcgc cgacgcgcca ctgttcggcg
      181 tcgtcagccg cctgacctcg cagaaggggc tggacctcgt gctcggcgcc ttgcccggcc
      241 tgctcaagcg cggcggccag ctcgcgctgc agggcgcggg cgacgcggcg ctcgaggcgg
      301 cgttcgtcgc cgccgccaag gcccacccgg gcgaggtcgc ggtgcgcatc ggctacgacg
      361 aggccttcgc gcaccggctg atcgccggcg ccgacgcgat cctcgtgccc tcgcgcttcg
      421 agccctgcgg cctgacccag ctctacggcc tgcgctacgg cacgctgccg gtcgtgcgcc
      481 gcacgggcgg cctggccgac accgtggtcg atgcgaacga cgaggcgctg caggccgatc
      541 gcgccaccgg cttcgccttc gacgccgcga cgccggccgc gctcgacgcc gccttgcagc
      601 gcgccggcac gctctacgcg cagcccgagc gctggcgccg cctgatggcg cgcgcgatgg
      661 cgcaggactt ctcgtggggt ggtgcggcgc agaaatacat cgcgctgtac cgcgatctga
      721 tccggccgaa atagagggtg ctgccggtgc tgttcgcccg atcgggccgg cgcggaccga
      781 acgatcatgg gcgcatgcga acgattcgac tctcggatcc cttcccgcgc ggcaagcccg
      841 cgaagaagct cgaatggggc agcagcgcct gggccgccga ctcgtgcctg gtcgagtggt
      901 cgcgcttcga gcgtgaatgg ctggagccga cgccgatcaa gcgggccgcg cccgaaccgg
      961 tctggaagtg gagcaccagc gcgctgccgc tgaacgtgtg gctgggcctg ggcctgctcg
     1021 gcgcagcgct gatctgggtg acctggggct gacccgcccc ggtgcctcga cagcgcggga
     1081 cgggtccccg tcagtttcgg cgacacacct ggccaggact gcctgccgcg ggcagcataa
     1141 tctcgccatt tcctgtccga catgggtccg caccacgcgt gctccaccgt gtcgcgcccc
     1201 cgtgtcgcag cgcccctcac ccgcgagtcg ttgcgtcgat gggcatcgga cgccggctgc
     1261 ccggaagggc ggtgcggcct gctctccaac ctctcgcgca acgagcccgc atgaccacca
     1321 cgattgccac gattcccttg tccgccgtgc gcgccacggc ttcccgcgtg tgcgctcccg
     1381 ctgcaacgag ctggcgcgtc gccgacatcg aagcgctctt cgacctgccc ttcatggacc
     1441 tgctgttccg cgcccagcag gcgcaccgcg agcaattcga tgcgagcgag gtgcaactct
     1501 ccaccctgct gtcgatcaag accggcggct gccccgaaga ctgcggctac tgcccgcagt
     1561 cggcccactt cgacaccggc gtcgaggcga gcaagctgat gccgctgcaa gaggtggtcg
     1621 acgcggcgca ggccgccaag gcacagggtg cgacgcgctt ctgcatgggc gccgcgtggc
     1681 gcagccccaa agcccgcgac atcgaacgcg tcagcgaaat ggtgcgcgag gtcaaggcgc
     1741 tcgggctgga gacctgcatg acgctgggca tgctcgacgc cgatcaggcg caggccctcg
     1801 acgccgccgg cctggactac tacaaccaca acctcgacag cgcgcccgag ttctacggcc
     1861 agatcatcgg cacccgcacg taccaggatc ggctcgacac cctcgcccac gtgcgccagg
     1921 ccggcatcaa ggtgtgcagc ggcggcatcg tcggcatggg cgagagccgg gcgcagcgcg
     1981 ccgggctgat cgcgcagttg gccaacctcg atccctaccc cgagtcggtg ccgatcaaca
     2041 acctcgtgca ggtcgaaggc acgccgctgg cgggcaccgc gccgctcgac ccgttcgagt
     2101 tcgtgcgcac gatcgcggtg gcgcgcatca cgatgcccaa gacgatggtg cgcctgtcgg
     2161 ccgggcgcga gcagatggac gacgcgctgc aggcgctgtg cttcgccgcg ggtgccaact
     2221 cgatcttcta cggcgatcgg ctgctgacca cggccaaccc gcaggccgcg cgcgaccggg
     2281 cgctgttcga gcggctcggc ttgcgagcga tgacatgagc gacgtgcgca agccgatgac
     2341 cctgcatcgc ctgcgtgcga tgcacaccca aggcgagaag atcgcgatgc tgacctgcta
     2401 cgacgccagc ttcgcgcggc tgctcgacgc ggccggcgtc gacgtgctgc tggtcggcga
     2461 ttcgctcggc atggtgctgc agggccatga cagcacggtg ccggtgtcgc tcgacgagat
     2521 ggcgtatcac acgcaatgcg tggcacgcgg caacgcgcat gcgtggatca tcggcgacct
     2581 gccgttcggc agctaccacg cctcgttcga ccaggccatg cacggcgctg cgcgcctgat
     2641 gcaaagcggc gcgcacatgg tcaagctcga aggcggcggc tggaccgccg agaccgtgcg
     2701 cttgctggtc gagcgcggca tcccggtctg cgcgcacctg ggcctcacgc cacagtcggt
     2761 gcacgccctc ggcggctacc gcgtgcaggg gcgcgacgag caggccgcgc agaccctgcg
     2821 gcgccacgcg cgcgagctcg ccgaggccgg cgcgtcgatg ctggtgctcg aactgatgcc
     2881 gagcgcgctc gccggcgaac tcacagcggc gctgccgatt cccgtgatcg gcatcggcgc
     2941 cggcgcggcc tgcagcggcc aggtgctggt gctgcacgac atgctgggca tcacgcaggg
     3001 cacgctgccg cgcttcgtgc gcgacttcat ggccgggagc gccggcatcg agcaggcatt
     3061 gcgtcggtac gtcgcggccg tcaaggacgg cagcttcccc gtcgaatcca tccacaccta
     3121 ctgagtcgct ctcgacacac attcagcatg cgcgtcatcc acaccatcgc cgacttacgc
     3181 caagccctcg ccggcatcga cggcgtcgcg ttcgtgccca cgatgggcaa cctgcacgcc
     3241 ggccacctct cgctgatcga acgcgccaag caggccggcg ggccggtcgt cgcgagcatc
     3301 ttcgtcaacc ggctgcagtt cgcgccgcac gaggacttcg acacctaccc gcgaaccttc
     3361 gagcgcgact gcgaactgct gcgcggcgcc ggctgcaact tcgtgttcgc gcccgatgaa
     3421 gccgagctct atcccgagcc tcaggcctac accgtgcagc cgccagccgc gctcgccgac
     3481 atcctcgaag ggcacttccg ccccggcttc ttcaccggcg tgtgcaccgt cgtgttgaag
     3541 ctgttcaact gcgtgcagcc gcgcgtggcg gtgttcggca agaaggacta ccagcagctg
     3601 atggtgatca gccggatggt gcggcagttg gcgttgccga tcgagatcgt tgccggcgag
     3661 acgacgcgtg ccgaagacgg cctggcgctg tcctcgcgca acggctatct cgacgcggca
     3721 cagcgggccg aggcggtgac cttgtcgcgc atcctgaagg ccatcgccga cgacgtgcgg
     3781 cgcgggcggc gcgactggcc ggcgctggag gacaccgcga tgcggtcgct caccgcgcga
     3841 ggctggcagc cggactatgt cgcggtccgc cggcgcgccg acctggcgct gcccacgacc
     3901 ggtgacgcgc tggtcgtggt ggcggctgcc aggctcggat cgacaaggct gatcgacaac
     3961 ctcgaactcg acgtgccggc gtagagcgac gcggacgcgc ctaggcatcg ccatggcgaa
     4021 gcggtccgcg gccgccctga agcgtgacaa tgtgggtgac ggccagacgc ttccgcggaa
     4081 acggccgatc agttggaggc ggcatggcgc agcttatcga gcatctcgcg gacctgacgg
     4141 gatatcgcga ccgggatcaa ctggacgtga cggtcgtgcg ggcgatcaag gatgtgctgc
     4201 gcccgcgctg cgtcagcatc tatcgctgca tcggagagcc gggcgcgcag cgctggctga
     4261 cgcgcgcgcg catgggcccg ggggatgcca cggccatggc agatccgttg tcggccgaaa
     4321 tctcggcgct gccgcacctg gacgacgtgc cggcgcggcg cgactgcgtg acgcgggccg
     4381 aggtcgtcac ggtgggtgcg gggccgcagc tcaacgactt tccgatcgcg acggagcggg
     4441 aggtcatcgg cgtcctcgag ctcgagaccg ccgaggcgct gacggccagc gaccgggcga
     4501 tgatccgtac gatcctgcgc atctatggca acttccacga cctgctcgat cacagcgagc
     4561 gcgacccgct gaccgggctg ctgaaccggc agaccttcga tgcggcgttc atgactcgcg
     4621 ccgcaagctc gcaccccggg gacgaggcgg ccgtgtcggg ccgacgcgcc gcagcagtgt
     4681 cggagtcggc ggtctggctg ggggtcgtcg acatcgacca cttcaagcat gtcaacgaca
     4741 cctacggcca cctgatcggc gacgaagtgc tgttgctgct gtcccgcttg ttgcggtcga
     4801 cgtttcgaca ccatgaccgc ctg
//
LOCUS       DATMJB010000176         3535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_688427, whole genome shotgun sequence.
ACCESSION   DATMJB010000176 DATMJB010000000
VERSION     DATMJB010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688427"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VNU48_03920"
     CDS             <1..487
                     /locus_tag="VNU48_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWI10454.1"
                     /translation="LRVFIETPLGRAQRCVAMYDAGLDRMVGAADILHPDDNPYLERL
                     QRDGCVAIGQVAADPISAGPLQSYLEAAKVVSLMDMSFSVNGALFGTFSCEQVGTPVE
                     WAQRQQQTLRKIASRASLTLMHVVNAAIDTTPGALWETSTPNRLATMPMPLDLLPPKA
                     E"
     gene            complement(503..1270)
                     /locus_tag="VNU48_03925"
     CDS             complement(503..1270)
                     /locus_tag="VNU48_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI10455.1"
                     /translation="MSGLRVEGVSVALGSTRALREVSLAFAPGWTAIVGPNGAGKSTL
                     LRVLAGLQPVDAGAVRFDGRPLAALRPRERATRIAWLAQQGEASGELTVREVVHLGRL
                     ARLGLFTAPGAIDEARVDAAMQEAECGAWQQRRLHELSGGERQRVLLARALAVDAPWL
                     LLDEPTTHLDPPHQVALVRLVHRQLNAGTTVVSVLHDLSLALLADTLVVMQQGRIRAQ
                     GARDDPQLHAALREVFDDAIRIERFGARWIAIPHVEP"
     gene            complement(1267..2226)
                     /locus_tag="VNU48_03930"
     CDS             complement(1267..2226)
                     /locus_tag="VNU48_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWI10456.1"
                     /translation="MALLVAGLAAGSEGWSLPDLARTWAGPDGVLIVGQIRAPRTLGA
                     WLTGALLGLAGAIAQGLFRNPLADPYLLGSASGASLGVVVLLAASASGGHAIGLATAD
                     TLARIGLVGAAFGGALLGVLLTLTLARGAQQTTRLLLAGVVVGVMLAAASDLITSAVP
                     EALRGKQAFLLGSTGYLGWGSCAVLAIGLALTLPPSWRLARALDALTLGADTATSLGV
                     ALPRVRVALVAAIALATGLAVSQAGLIAFVGLVAPHLVRRFAPARHGFALLASAAAGG
                     TLLLLADVLARSLIAPKEVPVGVLTALLGGGYLVWLLHRRRVA"
     gene            complement(2283..3170)
                     /locus_tag="VNU48_03935"
     CDS             complement(2283..3170)
                     /locus_tag="VNU48_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HWI10457.1"
                     /translation="MTRRLAALLLGLAAVAAPAQPEVRDDRGATLRLAAPPQRIVSLL
                     PSLTESVCALGACARLVGVDRFSNWPGSVRGLPKLGGIDDAQIERIVALRPDVVLVSS
                     SARIVDRLEALGLKVLVLDSRDHADVRRSLIAIAQLLDTPREAERVWAGIERETQAAV
                     ERVPPALRGRRVYFEVDTTPFAAGAGSFIGETLARLGLANAVPVELGPFPQLNPEYVL
                     RAQPDIVMAVRPALAEMPRRPGWAALRALRAGRTCGFASDRYELLVRPGPRMGEAAGV
                     IADCLVAIAASDAAAKGAR"
     gene            complement(3209..>3535)
                     /locus_tag="VNU48_03940"
     CDS             complement(3209..>3535)
                     /locus_tag="VNU48_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWI10458.1"
                     /translation="GNDVPLTPRRTVALRADWSPAAGHRIDAGVNVVSAQRPDFENAC
                     RMPSYTTADLRYAYQWRLAELALGVSNLADAKYYTQAFACVGGTVESIYPEAGRAVTV
                     SMRLHF"
BASE COUNT          520 a         1358 c         1262 g          395 t
ORIGIN      
        1 cctgcgtgtc ttcatcgaaa ccccgctggg tcgcgcgcag cgctgcgtgg cgatgtacga
       61 cgccggcctg gaccgcatgg tcggcgccgc cgacatcctg caccccgacg acaaccccta
      121 cctggagcgg ctgcaacgcg acggctgcgt cgcgatcggc caggtggccg ccgacccgat
      181 cagtgccggc ccgctgcaga gttacctcga ggctgcgaag gtcgtctcgc tgatggacat
      241 gagcttctcg gtcaacggtg cgctgttcgg caccttcagt tgcgagcagg tcggcacgcc
      301 cgtcgaatgg gcccagcgcc agcagcagac gctgcgcaag atcgcctcgc gtgccagcct
      361 gacgctgatg cacgtcgtca acgcagcgat cgacaccacg ccgggggcgc tctgggagac
      421 cagcaccccg aaccggctgg cgacgatgcc gatgccgctg gacctgctgc cgcccaaggc
      481 cgaatagaag ccgtcgcgcc ggtcacggct cgacgtgcgg gatcgcgatc cagcgcgcac
      541 cgaagcgctc gatccggatc gcgtcgtcga acacctcgcg cagcgccgca tgcagttgcg
      601 ggtcgtcgcg cgcgccctgc gcgcggatgc ggccctgctg catcacgacc aatgtgtcgg
      661 caagcagcgc cagcgacaag tcgtgcagca cgctgaccac ggtcgtgccg gcgttcagct
      721 ggcggtgcac caggcgcacc agcgcgacct gatgcggcgg gtccaggtgc gtggtcggct
      781 cgtcgagcag cagccacggc gcgtcgaccg cgagcgcgcg cgccagcagc acgcgctggc
      841 gctcgccgcc cgacaactcg tgcaggcggc gctgctgcca ggcgccgcat tcggcctcct
      901 gcatcgcggc atcgacgcgc gcttcgtcga tcgcgccggg cgcggtgaac aggcccagcc
      961 gcgccagccg gccgaggtgc acgacctcgc gcaccgtgag ctcgccgctg gcctcgccct
     1021 gctgggccag ccacgcgatc cgcgtggcgc gttcgcgcgg gcgcagcgcg gcgaggggcc
     1081 ggccgtcgaa ccgtaccgca ccggcgtcga ccggttgcag cccggccagc acgcgcagca
     1141 gcgtggactt gccggcgccg ttcgggccga cgatcgcggt ccagccaggc gcgaacgcga
     1201 ggctcacctc acgcagggcc cgcgtgctgc cgagcgcgac gctgacgccc tcgacgcgca
     1261 agccgctcat gccacgcgcc gccggtgcag caaccagacc aggtagccgc cgccgagcaa
     1321 tgccgtgagc acgccgaccg gcacttcctt cggcgcgatc aggctgcgcg cgagcacgtc
     1381 ggccagcaac agcaaggtgc caccggccgc ggcgctggcg agcagcgcaa agccgtggcg
     1441 cgcgggcgcg aagcggcgca ccaggtgcgg cgccaccagg ccgacgaacg cgatcagccc
     1501 ggcctgcgac accgccaggc cggtggccag cgcgatcgcc gcgaccagcg cgacgcgcac
     1561 cctcggcaag gccacgccga ggctggtcgc ggtgtcggcg ccgagcgtca gcgcatcgag
     1621 cgcacgcgcc aggcgccagc tcggcggcaa ggtcagcgcg aggccgatcg ccagcaccgc
     1681 gcaactgccc cagccgaggt agccggtgct gccgagcaga aaggcctgct tgccgcgcag
     1741 cgcctcaggg accgcgctgg tgatgaggtc gctggcagcc gcgagcatga caccgacgac
     1801 gacgccggcc agcagcaggc gtgtcgtctg ctgcgcgccg cgcgccagcg tcagggtcag
     1861 cagcacgccg agcaaggcac cgccgaacgc cgcaccgacc aggccgatgc gcgccagcgt
     1921 gtcggctgtg gcgaggccga tcgcgtgccc gccgctggcg ctcgctgcca gcagcacaac
     1981 cacgccgagc gacgcgcccg aggccgaacc gagcaggtac gggtcggcca gcggattgcg
     2041 gaacagcccc tgcgcgatcg cgcccgccag gcccagcagt gcgccggtga gccaggcgcc
     2101 gagcgtgcgc ggggcgcgta tctggccgac gatcagcacg ccgtcggggc cggcccaggt
     2161 ccgggccagg tccggcaacg accagccctc gctgccggcc gccagcccgg ccaccagcaa
     2221 cgccatggtc gccagcgtga gcccgagcgc gaggcggcga cggcgctgcg catcggtcgc
     2281 cgtcaccgcg cccccttcgc cgcagcgtcg ctcgcggcga tcgcgaccag gcagtcggcg
     2341 atcaccccgg ccgcctcgcc catgcgcggc cccgggcgca ccagcagctc gtagcgatcg
     2401 ctcgcgaagc cgcaggtccg acccgcgcgc agcgcgcgca gcgcggccca gccggggcgc
     2461 ctgggcatct cggcgagcgc cggccgcacc gccatcacga tgtcgggctg ggcgcgcagc
     2521 acgtactccg ggttcagctg cggaaacggc ccgagttcga ccggcaccgc gttcgccaga
     2581 ccgagccgcg ccagcgtctc gccgatgaac gagccggcac cggccgcgaa cggcgtggtg
     2641 tcgacctcga agtagacgcg ccggccacgc agcgccggcg gcacgcgctc gaccgccgcc
     2701 tgcgtctcgc gctcgatgcc ggcccagacg cgctcggcct ccctcggcgt gtccagcagc
     2761 tgcgcgatcg cgatcaggct gcgccgcacg tcggcgtggt cgcgcgagtc cagcaccagc
     2821 accttcaggc ccaacgcctc gagccggtcg acgatgcgcg ccgagctcga caccagcacc
     2881 acgtcgggcc gcagcgcgac gatgcgctcg atctgcgcgt cgtcgatgcc gccgagctta
     2941 ggcagcccac gcacgctgcc cggccagttc gagaaacgat cgacgccgac cagccgcgcg
     3001 caggcaccga gggcgcagac gctttcggtc agcgacggca gcaggctgac gatgcgctgc
     3061 ggcggtgcgg ccaggcgcag cgtcgcgccg cgatcgtcgc gcacctccgg ctgcgccggc
     3121 gccgccaccg ccgccagacc gagcagcagt gccgcgaggc ggcgcgtcat cggcgcgttc
     3181 gttggcctgc gcaccacgtc gctcccggct agaagtgcag ccgcatcgac acggtgacgg
     3241 cgcgccccgc ctccgggtag atcgactcga ccgtgccgcc gacgcacgcg aacgcctgcg
     3301 tgtagtactt ggcgtcggcc agattcgata cgccgagcgc cagctccgcg aggcgccatt
     3361 gataggcgta gcgcaggtcg gcggtggtgt agctcggcat gcggcaggcg ttctcgaagt
     3421 ccgggcgctg cgcggacacg acgttgacgc cggcgtcgat ccggtggccg gcggccggcg
     3481 accagtcggc gcgcagcgcc accgtgcggc gcggcgtcag cggtacgtcg ttgcc
//
LOCUS       DATMJB010000177         3751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_332783, whole genome shotgun sequence.
ACCESSION   DATMJB010000177 DATMJB010000000
VERSION     DATMJB010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332783"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="VNU48_03945"
     CDS             <1..614
                     /locus_tag="VNU48_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWI10459.1"
                     /translation="AQALRALSAQVWVTEIDPINALQAAMEGYRVVTMEYAADKADIF
                     VTTTGNKSIITHAHMTAMKDQAIVCNIGHFDNEIDIASIEKYEWEEIKPQVDHVIFPD
                     GKKIILLAKGRLVNLGCGTGHPSFVMSSSFANQTIAQIELFTHSDYYEVGKVYVLPKH
                     LDEKVARLHLKKVGAMLTELTDEQAAYIGVPKQGPYKPDSYRY"
     gene            619..1374
                     /locus_tag="VNU48_03950"
     CDS             619..1374
                     /locus_tag="VNU48_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWI10460.1"
                     /translation="MRADQLLVQRALAPTRSAAQRLIDAGAVRWLGPKGWAVPRKAGE
                     DLPEACRLEVTDDAELRFASRGGLKLDAALTHCGVDVDAANCLDVGQSTGGFTDALLQ
                     RGAARVVGLDVGRGQLAATLAGDARVRSFEGLNARDVAGSEFEQAEPAHSFDLVVADL
                     SFISLTLVLGTIARYLAPTGNALLLVKPQFELQPADIGKRGLVKQVGAYARVEQRTRE
                     ACAAQALAVRDYFASAVVGGDGNQEFFVWARPA"
     gene            1388..2224
                     /gene="metF"
                     /locus_tag="VNU48_03955"
     CDS             1388..2224
                     /gene="metF"
                     /locus_tag="VNU48_03955"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HWI10461.1"
                     /translation="MSKTIPPISLEFFPPKTPEGVVKLAAVRRQLYALKPQFCSVTYG
                     AGGSTQDGTFAAVRAILDEGCEAAPHFSCIGASRESIRARLAQFSAHGIRRIVALRGD
                     LPSGYGGSNGEFHYASDLVAFIRAETGDRFHIEVAAYPEMHPQSRSPQADLQAYVVKV
                     KAGASSAITQMFFNADAYFRFVDEAHALGATIPVVPGIMPITNANGIIRFADSCGADM
                     PRWIRLRLQGFGDDTDSIKAFGLDVISTLCEQLRAGGAPGLHFYTMNQSTATLDLMRR
                     LG"
     gene            complement(2234..2815)
                     /locus_tag="VNU48_03960"
     CDS             complement(2234..2815)
                     /locus_tag="VNU48_03960"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HWI10462.1"
                     /translation="MQQPAPDRAALRAKLIAARLALTDRLERAVQLQGALRVWLVGRR
                     ETTIGAYWPIKGEFDPLPALYRWAEGAPDGIVRRIGLPVANRETGQLRFHVWYPGCPM
                     EHDAYDIPKPKDTEEFAPAVLIVPCLGFGPGGVRLGYGGGFFDRTLAALRPEPVTVGV
                     GFTQGFLPLLRAGPLDVQLDAMLTEDGLMWQRP"
     gene            2866..3285
                     /locus_tag="VNU48_03965"
     CDS             2866..3285
                     /locus_tag="VNU48_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HWI10463.1"
                     /translation="MSAVADEFVAHCTELLASAGTVRSRRMFGGRGFYIDELFVALIA
                     YDRLYLKADAQTRPAFEAAGCTPFMYDRPGDGRSVTVGYFSAPDDAMESPVLMQPWAR
                     LALAAALRARAAKPAARRPARKATPRSGSAKTAPTKL"
     gene            3390..3590
                     /locus_tag="VNU48_03970"
     CDS             3390..3590
                     /locus_tag="VNU48_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10464.1"
                     /translation="MIAQVVRTRQPDAAVEHRQLAAQRPQTRGIGMELEGSAMKQLEL
                     RIHHQSPALGVAMIGHCAFGIQ"
     gene            3622..>3751
                     /locus_tag="VNU48_03975"
     CDS             3622..>3751
                     /locus_tag="VNU48_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWI10465.1"
                     /translation="MSSGSRRPLSLGNLRAFEAVARRLSFSEAAEELFVTQSAISRQ"
BASE COUNT          603 a         1297 c         1290 g          561 t
ORIGIN      
        1 cggcccaggc cctgcgcgcg ctgagcgccc aggtgtgggt gaccgagatc gacccgatca
       61 acgcactgca ggccgcgatg gaaggctacc gcgtcgtgac catggagtac gcagccgaca
      121 aggccgacat cttcgtcacc accaccggca acaagtcgat catcacgcac gcccacatga
      181 cggcgatgaa ggaccaggcg atcgtctgca acatcggcca cttcgacaac gagatcgaca
      241 tcgcctcgat cgagaagtac gaatgggaag agatcaagcc gcaggtcgac cacgtgatct
      301 tccccgacgg caagaagatc atcctgctcg ccaagggacg cctggtgaac ctgggctgcg
      361 gcaccggtca cccgagcttc gtgatgtcga gctcgttcgc gaaccagacg atcgcgcaga
      421 tcgagctgtt cacgcacagc gactactacg aagtcggcaa ggtctacgtg ctgcccaagc
      481 acctggacga gaaggtcgcg cggctgcacc tgaagaaggt cggcgcgatg ctgaccgagc
      541 tcaccgacga gcaggctgcc tacatcggcg ttccgaagca gggcccgtac aagcccgatt
      601 cgtaccgcta ctgatccggt gcgcgccgat caactgctgg tgcagcgcgc cctcgcgccc
      661 acgcgttcgg ccgcccagcg gctgatcgat gcgggcgccg tgcgctggct cggcccgaag
      721 ggctgggccg tgccgcgcaa ggcgggcgaa gacctgcccg aggcgtgccg gctcgaggtc
      781 accgacgacg ccgagctgcg cttcgcatcg cgcggcggcc tgaagctgga cgcggcgctg
      841 acgcattgcg gcgtcgacgt cgatgcggcg aactgcctcg atgtcggcca gagcaccggc
      901 ggcttcaccg atgcgctgct gcagcgcggc gcggcgcgtg tcgtcggcct cgacgtgggc
      961 cgtggccaac tggcagcgac gctggccggc gacgcgcgcg tgcgcagctt cgagggcctg
     1021 aatgcgcgcg acgtggcagg ctccgaattc gagcaggccg agccggcgca ttcgttcgat
     1081 ctggtcgttg cggacctgtc gttcatctcg ttgacgctgg tgctgggcac gatcgcgcgc
     1141 taccttgcgc cgaccggcaa cgcgctgttg ctggtcaaac cgcagttcga actgcagccg
     1201 gccgacatcg gcaagcgcgg gctggtgaag caggtcggcg cgtacgcgcg cgtcgaacag
     1261 cgcacccgcg aggcgtgcgc ggcgcaggcg ctggccgtgc gcgactactt cgccagcgcg
     1321 gtcgtcggcg gcgacggcaa tcaggaattc ttcgtgtggg cacggcccgc atgacaagga
     1381 agtcgatgtg agcaagacga ttcccccgat cagcctggag ttcttcccgc ccaagacgcc
     1441 cgagggcgtg gtcaagctcg ccgcggtgcg ccggcagctc tacgcgctga agccgcagtt
     1501 ctgctcggtg acctacggcg ccggcggttc gacgcaggac ggcaccttcg ccgcggtgcg
     1561 ggcgatcctc gacgagggct gcgaggccgc gccgcacttc tcgtgcatcg gcgcgagccg
     1621 cgagtcgatc cgcgcgcggc tggcgcagtt cagcgcgcac ggcatccgcc gcatcgtcgc
     1681 cttgcgcggc gatctgccga gcggctatgg cggcagcaac ggcgaattcc actacgccag
     1741 cgacctggtc gcgttcatcc gcgccgaaac cggcgaccgc ttccacatcg aggtcgccgc
     1801 gtaccccgag atgcacccgc agtcgcgttc gccgcaggcc gacctgcagg cctatgtcgt
     1861 gaaagtgaag gccggggcga gttcggcgat cacgcagatg ttcttcaacg ccgatgcgta
     1921 cttccgcttc gtcgacgagg cgcacgcgct gggcgcgacg atcccggtcg tgccgggcat
     1981 catgccgatc acgaacgcga acggcatcat ccgcttcgcc gacagctgcg gcgccgacat
     2041 gccgcgctgg atccggctgc gcctgcaggg cttcggcgac gacaccgact cgatcaaggc
     2101 cttcgggctg gacgtgatct cgacgctgtg cgagcagctg cgtgccggcg gcgcgccggg
     2161 gctgcacttc tacacgatga accagagcac cgcgacgctg gacctgatgc gccgcctcgg
     2221 ctaggacact cggctagggc cgctgccaca tcaggccgtc ttcggtgagc attgcgtcca
     2281 gctgcacgtc gagcgggccc gcgcgcagca gcggcaggaa gccctgcgtg aagccaaccc
     2341 ccaccgtcac cggctcgggt cgcagcgccg cgagcgtgcg gtcgaagaag ccgccgccgt
     2401 agcccagccg cacgccgccg gggccgaagc cgaggcaggg cacgatcagg accgcggggg
     2461 cgaactcctc ggtgtccttg ggcttcggga tgtcgtaggc atcgtgctcc atcgggcagc
     2521 ccgggtacca gacatggaag cgcagctgcc cggtctcgcg gttcgcgacc ggcaggccga
     2581 tgcgccgcac gatcccgtcg ggtgcgccct cggcccagcg gtacagcgcc ggcaacggat
     2641 cgaactcgcc cttgatcggc cagtaggcgc cgatcgtcgt ctcgcggcgc cccaccagcc
     2701 agacgcgcag cgcaccttgc agctgcaccg cgcgctcgag ccggtcagtc agcgcgagac
     2761 gcgccgcgat cagtttcgcg cgcaaggcgg cgcggtcggg cgcaggttgc tgcatgcagt
     2821 ctccatccgg ttgaggttcg cgctgcgatt gtgcaagact gcgccatgag cgccgtcgcg
     2881 gacgaatttg ttgcccactg caccgagctg ctggcatcgg cgggcacggt ccgctcgcgc
     2941 cggatgttcg gcgggcgcgg cttctacatc gacgagttgt tcgtcgcgct gatcgcctac
     3001 gaccgcctgt acctgaaagc cgatgcgcag acgcgcccgg ccttcgaggc ggccggctgc
     3061 acgccgttca tgtacgaccg acccggcgac ggcaggtcgg tgacggtcgg ctacttcagc
     3121 gcgcccgacg acgccatgga gtcgccggtg ctgatgcagc cctgggcccg gttggcgctc
     3181 gccgccgcgc tgcgcgccag ggccgcgaag ccggcggcga ggcggcccgc caggaaggcc
     3241 acgccgcgca gcggctcggc caagaccgcg ccgacaaagc tctgacgccg cgggttccag
     3301 cgcaccgtca cgctggcgcc ggtacgcgcg ggttcgatca ctgcgttgtc gtcggcggcc
     3361 aggcgcacac gctcgccggc ctcgacgaaa tgatcgccca ggtcgttcgc acgcgtcagc
     3421 cagacgcggc cgtcgagcac cgtcagctcg ccgcccagcg gccccagacg cgcggcatcg
     3481 gaatggagct ggaaggttcc gccatgaaac agctggaatt gcgtattcat catcaatctc
     3541 ctgcacttgg tgtcgctatg atcggccatt gcgcctttgg aatccaatga aactcgggtc
     3601 ggccattgat gcggatttga tatgagcagc ggctcgcgtc gcccgctctc gctgggcaat
     3661 ctgcgcgcct tcgaggcggt ggcgcggcgg ctgagcttca gcgaggccgc cgaggagctg
     3721 ttcgtgacgc agtcggcgat cagccggcag a
//
LOCUS       DATMJB010000178         2397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_95632, whole genome shotgun sequence.
ACCESSION   DATMJB010000178 DATMJB010000000
VERSION     DATMJB010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95632"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VNU48_03980"
     CDS             <1..209
                     /locus_tag="VNU48_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016724589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI10466.1"
                     /translation="PEFVTVKRPTLVIWGEDDIALPVALVDGLDAFVPEMRLVRVPGA
                     THWIVHERPQFIAAEIERHLADTQ"
     gene            complement(324..1973)
                     /gene="groL"
                     /locus_tag="VNU48_03985"
     CDS             complement(324..1973)
                     /gene="groL"
                     /locus_tag="VNU48_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345520.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HWI10467.1"
                     /translation="MAAKDVIFGGEARARMVEGVNILANAVKVTLGPKGRNVVLERSF
                     GGPTVTKDGVSVAKEIELKDKLQNMGAQMVKEVASKTSDIAGDGTTTATVLAQAIVRE
                     GMKYVAAGMNPMDLKRGIDKAVTALVEQLKKQSKATTTSKEIAQVGTISANSDEEVGK
                     IIADAMDKVGKEGVITVEDGKSLNSELEVVEGMQFDRGYLSPYFINNPEKQTAILDNP
                     FVLLFDKKISNIRDLLPTLEQVAKSGRPLLIVAEEVEGEALATLVVNTIRGILKVVAV
                     KAPGFGDRRKAMLEDIAILTGGKVIAEEVGLTLEKVTLADLGQAKRVEVGKENTTIID
                     GSGAAGDIEARVKQVRIQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALLRAKQAAGVIKGDNPDQEAGIKLVLKAIESP
                     LREIVFNAGGEASVVVAAVLAGKGNYGFNAANDTYGDMIEMGILDPTKVTRTALQNAA
                     SVASLMLTTEAMIAEAPKEESAGGGMPGGMGGMGGMGGMDM"
     gene            complement(2001..2291)
                     /locus_tag="VNU48_03990"
     CDS             complement(2001..2291)
                     /locus_tag="VNU48_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWI10468.1"
                     /translation="MKLRPLHDRVIVKRLEQETKTASGIVIPDNAAEKPDQGEVLAVG
                     PGKRSEKGDFIVLNIKVGDRVLFGKYSGQTVKVDGEELLVMKEDDLFAVVEK"
BASE COUNT          340 a          798 c          728 g          531 t
ORIGIN      
        1 cgcccgagtt cgtgacggtc aagcggccca cgctggtgat ctggggcgag gacgacatcg
       61 cgctgccggt cgcgttggtc gacgggctcg acgcgttcgt gcccgagatg cggctcgtgc
      121 gcgtgcccgg cgcgacgcac tggatcgtgc atgagcggcc acagttcatc gcggccgaaa
      181 tcgagcgcca tctcgcggac acgcaatgaa aagagggcgc cgaagcgccc tctttgacct
      241 gccgggaagt tgagcaccgg tcgcaaccga ccgatgttcg aacgccccga gttattgcgg
      301 cagcgaagct gtccgcaatg acatcacatg tccatgccgc ccatgccacc catgccgccc
      361 atgccacccg gcatgccgcc gccggccgac tcttccttcg gcgcttcggc gatcatcgct
      421 tcggtggtca gcatcagcga agcgaccgag gctgcgttct gcagcgccgt gcgcgtcacc
      481 ttggtcgggt ccaggatgcc catttcgatc atgtcgccgt aggtgtcgtt ggcggcattg
      541 aagccgtagt tgcccttgcc ggccagcacc gcagcgacga ccacgctggc ctcgccgccg
      601 gcgttgaaga cgatctcgcg cagcggcgat tcgatggcct tcagcaccag cttgatgccg
      661 gcttcctgat cggggttgtc acccttgatc acgcctgccg cctgcttggc gcgcagcagc
      721 gccacgccac cgccggccac gatgccttct tcgacggcgg cacgggtcgc gtgcagcgca
      781 tcttcgacgc gggccttctt ttccttcatc tcgacttcgg tggcggcgcc aaccttgatc
      841 accgccacac cgccggccag cttggccacg cgctcttgca gcttctcgcg gtcgtagtcg
      901 ctggtggctt cttcgatctg gatgcgcact tgcttgacgc gcgcttcgat gtcgccagcg
      961 gcgcccgaac cgtcgatgat ggtggtgttt tccttgccca cttcgacgcg cttggcctgg
     1021 cccaggtcgg ccagcgtgac cttctcgagc gtcaggccga cttcttcggc gatgaccttg
     1081 ccgccggtca ggatcgcgat gtcttcgagc atcgccttgc gacggtcacc gaagcccggc
     1141 gccttgacgg cgacgacctt caggatgccg cggatcgtgt tcacgaccag cgtcgcgagc
     1201 gcttcgcctt cgacttcttc ggccacgatc agcagcggac ggccgctctt ggccacttgc
     1261 tccagcgtcg gcagcaggtc gcggatgttg ctgatcttct tgtcgaacag cagcacgaac
     1321 gggttgtcca gaatcgcggt ttgcttttcc gggttgttga tgaagtaggg cgacaggtag
     1381 ccgcggtcga actgcatgcc ttcgacgact tcgagttcgc tgttcaggct cttgccgtct
     1441 tcgacggtga tgacgccttc cttgccgacc ttgtccatcg cgtcggcgat gatcttgccg
     1501 acttcttcgt cgctgttggc cgagatcgtg ccgacctgcg cgatttcctt gctggtcgtc
     1561 gttgccttcg actgcttctt cagctgctcg accaaggccg tcacggcctt gtcgatgccg
     1621 cgcttcaggt ccatcgggtt catgcccgcg gccacgtact tcatgccttc gcgaacgatg
     1681 gcctgggcca gcacggtcgc ggtggtggtg ccgtcgccgg cgatgtcgct ggtcttggaa
     1741 gcaacttcct tgaccatctg cgcgcccatg ttctggagct tgtccttcag ctcgatttcc
     1801 ttcgcgaccg aaacgccgtc cttcgtgacg gtcgggccgc cgaacgaacg ctcgagcacc
     1861 acgttgcggc ccttcgggcc cagggtcacc ttgacggcgt tggccaggat gttcacaccc
     1921 tcgaccatgc gtgcgcgggc ttcgccgccg aaaattacgt cttttgctgc catatcaaat
     1981 tctcctgagt gcttgctgga ttacttctcg acgactgcga acaggtcgtc ttccttcatc
     2041 accagcagtt cttcgccatc gaccttgacg gtctggccgc tgtacttgcc gaacaacacg
     2101 cggtcgccga ccttgatgtt caggacgatg aaatcgccct tttcgctgcg cttgccgggg
     2161 ccgaccgcca gcacttcgcc ctgatcgggc ttctcggcgg cgttgtcggg gatcacgatg
     2221 cccgaggcgg tcttggtttc ttgttccagg cgcttgacga tcacgcggtc gtgcaacgga
     2281 cgaagtttca tgagatctcc ttggttcgaa tcgatcggcg gtcgcggctc ggggtcggca
     2341 tgggcggcct cggccccttg tccagtatta gcactcctct cggtcgagtg ctagcag
//
LOCUS       DATMJB010000179         5853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_12443, whole genome shotgun sequence.
ACCESSION   DATMJB010000179 DATMJB010000000
VERSION     DATMJB010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12443"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..861
                     /locus_tag="VNU48_03995"
     CDS             127..861
                     /locus_tag="VNU48_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWI10469.1"
                     /translation="MAQIDAAMRKSIEEKPLPSPVSAAYDAVLPSVVRVVGLMTEGDD
                     GSDRDEDKSDKGDKSAKERGPVERGIGTGVVIIDNGTILTNLHVINGAKRVKVTFMDG
                     TESDAVVVSTQPDNDLAVLRAKTIPDDLEAATLRNTADLAPGDQVMAVGFPFGIGPSV
                     SAGVVSGLKREFRSPEGRQTMTNLIQFDAAANPGNSGGPLVTMDGEVVGIVTAILNPS
                     RQRTFIGIGFAVPIENAAAAVGMPPF"
     gene            898..1902
                     /locus_tag="VNU48_04000"
     CDS             898..1902
                     /locus_tag="VNU48_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWI10470.1"
                     /translation="MDNTSVNSSSAHAATLMEQVLYEVKRVVVGQDHFLERVMVAVLA
                     QGHLLVEGVPGLAKTLTVKTLAATLRGSFKRIQFTPDLVPADLIGTRIFNPRTGEFST
                     SLGPVFTHLLLADEINRAPAKVQSALLEVMQERQVTIAGETHRVPEPFLVMATQNPIE
                     TEGTYPLPEAQVDRFMMKVLVDYPSEEEEFVIAQRVTGPATVVSAVASMGQLAELQQE
                     CRKVYVDPSLMQYAVKLVSATRRPERYDLADIAKYLSYGASPRATIGLIEGARALAFM
                     RGRGYALPEDMTVLVGDVLRHRLVLSYEALADGLTADGVVQRVMKKIPVPDKPLAPPA
                     "
     gene            1910..2884
                     /locus_tag="VNU48_04005"
     CDS             1910..2884
                     /locus_tag="VNU48_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWI10471.1"
                     /translation="MATSTPPSAEALLKRLEWTVIRRLDGLLQGDYRTLWRGAGLDLA
                     DLREYQHHDDVRHIDWNVTARLQQPYVRQFTEDRELTAWFLVDLSASVDFGSNELTKR
                     TIAREFVGVLARLLTRHGNRVGALLYGTKVDTVLPAGSGRKHVLNLLQRMAQRPEQSA
                     PGATDLRELLVAALGVLKRRSSVFVISDFISAPGWDEALARLAMRHDVTAVRLFDPLE
                     MELPDIGLVTMRDAESGEQLVVDTHDRGFRARFAQAAQRRETALLDGLTRAGVDTLEL
                     ATDAALTDAILRFADLRKQRSRLANWSGRPGRANMGAHLSSARKGVAA"
     gene            2920..3948
                     /locus_tag="VNU48_04010"
     CDS             2920..3948
                     /locus_tag="VNU48_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWI10472.1"
                     /translation="MSFLWPEMLWGLLALPLLVMLYVWLLRRRKKSVLRFASLSLVKE
                     AMGKGLAWRRHLPPALLLTAIAALLVAAARPAAVITLPQAEKTIVLAIDVSGSMRATD
                     VLPDRMTAAKEAAKAFVADLPRTVRIAVVQFAGTAAVVQAPTLSREDVVAAIDRFQLQ
                     RGTAIGSGIVLSLATLFPDAGIDLSQITGQREMPPAPGDKPRPPFTPVAPGSYGSAAI
                     ILLTDGQRTTGPDPLEAAKMAADRGVRVYTVGVGTKEGETIGFEGWSMRVRLDEETLK
                     GISNMTRADYFYAGTAADLKQVYQGLSTRLIVEKKETEISALFAAVGALLVMLAAGLS
                     VWWFGRVA"
     gene            complement(3964..5496)
                     /locus_tag="VNU48_04015"
     CDS             complement(3964..5496)
                     /locus_tag="VNU48_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HWI10473.1"
                     /translation="MLWIALHLPLLSLESFAATLPSAPGARPLALMDAHAILHVNAAA
                     RALGVRPGFKRATALALAPQLTFGQADRERDAQALAAVAHAALAFTPGVSIQPPSAAT
                     PDDTPHTVLLEVQASLRYFGGLAALRQRLQDALEPLGHRVHIVSARTALGAALLARVH
                     ARLDCADLGSTCAALDRVPVWLLGPGRAHWEALQGMGLRTIADLRGMPRDGLARRFGE
                     VVLDELDRASGTRPDPREPLALPASFDSRLELFARADTAEQLMHGAAALLARLVAWLS
                     AQHAFARRFTLRMEHEQRSRRDGPGAVTALDIALAEPSRDPAHLRVLLRERLAQLQLP
                     APTLELRLQATDIARRAPPNAELFPTPASEHEGLTRLIERLQARLGREQVLRLVPRHD
                     HRPERATAWRSADAALRPGRARGAADSAAVAFHAMRPVWLLQHAEPLHERDSTPLVDG
                     QALQLLSGPERIESGWWDAELVARDYFIAQLPGGALVWIYRARVPAVATGPSWFLQGR
                     FG"
     gene            complement(5569..>5853)
                     /locus_tag="VNU48_04020"
     CDS             complement(5569..>5853)
                     /locus_tag="VNU48_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10474.1"
                     /translation="PAFVLCELSAAQRPTAAPLRLALRPAGADALAVRVLKRRGPPLE
                     AALQLALPPVLSEAARQRAQAGIEGPALGHVPTPPPGSLRAATFVNLAAG"
BASE COUNT          846 a         2088 c         2136 g          783 t
ORIGIN      
        1 gcccgcctgc gggccgcgct gctgcgccgc gagcgggcgc tctgggcggc tgcggtgctg
       61 gtgctcctgg tggtcggcag cctggcgctg cgcggcgtga tcgcgccgcc gacaccggcg
      121 ctgacgatgg cgcagatcga cgccgcgatg cgcaagtcca tcgaggagaa gccgctgccc
      181 tcgccggtgt ccgcggccta cgacgcggtg ctgccctcgg tggtgcgtgt ggtcgggctg
      241 atgaccgagg gcgacgacgg cagcgaccgc gacgaggaca agagcgacaa gggcgacaag
      301 tccgccaagg aacgcggccc ggtcgagcgc ggcatcggca ccggcgtggt catcatcgac
      361 aacggcacga tcctgacgaa cctgcacgtg atcaacggcg ccaagcgcgt caaggtcacg
      421 ttcatggacg gcaccgagtc cgatgcggtg gtggtcagca cgcagcccga caacgacctc
      481 gcggtgctgc gcgccaagac catccccgac gacctcgagg ccgcgaccct gcgcaacacg
      541 gccgacctgg cgccgggcga ccaggtgatg gcggtgggct tcccgttcgg catcgggccc
      601 tcggtgtcgg ccggtgtggt gtcgggcctg aagcgcgagt tccgctcgcc cgagggccgg
      661 cagacgatga ccaacctgat ccagttcgac gccgcggcca accccggcaa ctcgggcggt
      721 ccgctggtga cgatggacgg cgaggtcgtc ggcatcgtca ccgcgatcct gaacccgtcg
      781 cgccagcgca ccttcatcgg catcggtttc gcggtgccga tcgagaacgc tgccgccgcc
      841 gtcggcatgc cgccgttctg acttcacccg gctccacgac accacgaaag cccgcgcatg
      901 gacaacacca gcgtcaacag cagcagcgcc cacgccgcga ccttgatgga gcaggtcctg
      961 tacgaggtca agcgtgtcgt cgtcggccag gaccactttc tggaacgcgt gatggtggcg
     1021 gtgctggcgc agggccacct gctggtcgag ggcgtgcccg ggctggcgaa gacgctgacc
     1081 gtgaagacgc tcgcggcgac gctgcgcggc agcttcaagc gcatccagtt cacgcccgac
     1141 ctggtgccgg ccgacctgat cggcacgcgc atcttcaacc cgcgcaccgg cgagttctca
     1201 acctcgctcg gcccggtgtt cacgcacctg ctgctggccg acgagatcaa ccgtgcgccc
     1261 gccaaggtgc agagcgcgct gctcgaagtg atgcaggagc gccaggtgac gatcgccggc
     1321 gagacgcacc gcgtgcccga gccgttcctc gtgatggcca cgcagaaccc gatcgagacc
     1381 gaaggcacct acccgctgcc cgaggcgcag gtcgaccgct tcatgatgaa ggtgctggtc
     1441 gactacccca gcgaggaaga ggagttcgtg atcgcgcagc gtgtcaccgg gcccgccacc
     1501 gtggtctcgg cggtcgcgag catgggccag ctcgccgagc tgcagcagga gtgccgcaag
     1561 gtctacgtcg acccgtcgct gatgcagtat gcggtcaagc tcgtcagcgc gacgcgccgg
     1621 cccgagcgct acgacctcgc cgacatcgcc aagtacctga gctacggcgc gagcccgcgc
     1681 gccacgatcg ggctgatcga gggcgcgcgt gcgctggctt tcatgcgcgg acgcggctac
     1741 gcgctgcccg aggacatgac ggtgctcgtc ggcgatgtgc tgcggcatcg cctcgtgctg
     1801 tcgtacgagg cgctcgccga tggcttgacg gccgacggtg tggtgcagcg cgtgatgaag
     1861 aagatcccgg tgcccgacaa gccgttggcg cccccagcct gattcgatca tggccacctc
     1921 cactccgccg tcggccgaag cgctgctcaa gcgcctcgag tggacggtca tccgccgtct
     1981 cgacggcctg ctgcagggcg actaccgcac gctgtggcgc ggcgccgggc tggacctggc
     2041 cgatctgcgc gagtaccagc accacgacga tgtgcgccac atcgactgga acgtgaccgc
     2101 acggctgcag caaccctatg tgcgccagtt caccgaagac cgcgagctca ccgcgtggtt
     2161 ccttgtcgac ctgagcgcga gcgtcgactt cggctcgaac gaactcacca agcggaccat
     2221 cgcgcgcgaa ttcgtcggcg tgctcgcgcg gctgctgacg cgccacggca accgcgtcgg
     2281 cgccctgctc tacggcacga aggtcgacac cgtgctgccg gccggcagcg ggcgcaagca
     2341 cgtgctgaac ctgctgcagc gcatggcgca gcggcccgag cagagcgcgc ccggcgcgac
     2401 cgacctgcgc gagctgctgg tcgccgcgct cggcgtgctg aagcgccgca gcagcgtgtt
     2461 cgtgatctcc gacttcatca gcgcgccggg ctgggacgag gccctggcgc ggctcgcgat
     2521 gcgccacgac gtgaccgcgg tgcggctgtt cgacccgctc gagatggagc tgcccgacat
     2581 cggcctggtg acgatgcgcg atgccgagag cggcgagcag ctggtggtcg acacccacga
     2641 ccgcggcttt cgcgcgcgct tcgcgcaggc cgcgcaacgg cgcgagaccg cgctgctcga
     2701 cggcctgacg cgcgccggcg tcgacacgct cgaactcgcc acggacgccg cgctgacgga
     2761 tgcgatcctg cgcttcgccg atctgcgcaa gcagcgcagc cggctcgcca actggagcgg
     2821 tcggcccggc cgcgcgaaca tgggtgcgca cctgtcgtcg gcgcgcaagg gagtggcggc
     2881 atgatcgatc tgccgttcgg cctgtcgttc cgctcgccgc tgagctttct gtggcccgag
     2941 atgctgtggg gcctgctggc gctgccgctg ctggtgatgc tgtatgtctg gctgctgcgc
     3001 cggcgcaaga agagcgtgct gcgcttcgcg agcctgagcc tggtcaagga ggcgatgggc
     3061 aagggcctgg cgtggcgccg ccacctgccg ccggctttgt tgctgaccgc gatcgccgcg
     3121 ctgctggtgg ccgcggcgcg gccggccgcg gtgatcacgc tgccgcaggc ggagaagacc
     3181 atcgtgctgg caatcgacgt ctccggcagc atgcgcgcca ccgatgtgct gccggaccgc
     3241 atgaccgccg ccaaggaggc cgcgaaggcc ttcgtcgccg acctgccgcg cacggtgcgc
     3301 atcgcggtcg tgcagttcgc cggcaccgcc gcggtggtgc aggccccgac gctgagccgc
     3361 gaagacgtgg tcgccgcgat cgatcgcttc cagctgcagc gcggcaccgc gatcggcagc
     3421 ggcatcgtgc tgtcgctcgc cacgctgttc cccgatgccg gcatcgacct gtcgcagatc
     3481 accggccagc gcgagatgcc gcccgcgccc ggcgacaagc cgcggccgcc gttcacgccg
     3541 gtggcgccgg gctcgtacgg ctcggccgcg atcatcctgc tgaccgacgg ccagcgcacc
     3601 accggccccg acccgctcga ggccgcgaag atggccgccg accgcggcgt gcgcgtctac
     3661 accgtgggcg tgggcaccaa ggagggtgag accatcggct tcgaaggctg gtcgatgcgc
     3721 gtgcgcctcg acgaggagac gctgaagggc atctccaaca tgacgcgtgc cgactacttc
     3781 tacgccggca ccgccgccga cctgaagcag gtctaccagg gcctgtcgac gcggctgatc
     3841 gtcgagaaga aggagaccga gatcagcgcg ctgttcgcgg ccgtcggcgc gctgctggtg
     3901 atgctggcgg cgggcctgtc ggtgtggtgg ttcgggcggg tggcctgatc catttcggtc
     3961 atctcaaccg aacctgccct gcagaaacca gctcggcccc gtggccaccg ccggcactcg
     4021 cgcgcggtag atccacacca gcgccccgcc cggcagctgg gcgatgaagt agtcgcgcgc
     4081 gacgagctcg gcgtcccacc agccggattc gatgcgctcg gggccgctga gcagctgcaa
     4141 ggcctggcca tcgaccagcg gcgtgctgtc gcgctcgtgc aagggctcgg cgtgctgcag
     4201 caaccacacc ggacgcatcg cgtgaaaagc caccgctgcg ctgtccgccg caccgcgcgc
     4261 acgccccggc cgcagtgccg catcggcgct gcgccaggcg gtcgcgcgct cggggcggtg
     4321 gtcgtgccgc ggcaccagtc gcaacacctg ctcgcggccg agccgtgcct gcagacgctc
     4381 gatcagacgc gtcaggcctt cgtgctcgga cgccggcgtc gggaacagct cggcattggg
     4441 cggcgcgcgc cgcgcgatgt cggtggcctg caggcgcagc tcgagcgtcg gcgcgggcag
     4501 ctgcagctgc gccaggcgtt cgcgcagcag cacgcgcagg tgggcggggt cgcgcgaggg
     4561 ctcggccagc gcgatgtcga gggcggtgac ggcgcccggt ccatcgcgac gcgagcgctg
     4621 ctcgtgctcc atccgcagcg tgaagcggcg cgcgaacgca tgctgcgccg acagccaggc
     4681 caccagccgc gccagcagcg ccgcggcgcc gtgcatcagt tgctcggcgg tgtcggcgcg
     4741 ggcgaagagt tcgagccggc tgtcgaagct ggccggcagc gcgagcggct cgcgcgggtc
     4801 gggccgggtg ccgctggcgc gatcgagctc gtcgagcacg acctcgccga agcgccgcgc
     4861 cagcccgtcg cgcggcatgc cgcgcagatc ggcgatcgtg cgcagcccca tgccctgcag
     4921 cgcctcccag tgcgcgcggc cggggccgag cagccagacc ggcacgcgat cgagtgcggc
     4981 gcacgtgctg ccgagatcgg cgcagtccag acgggcgtgc acgcgcgcga gcagcgccgc
     5041 gccgagcgcc gtgcgggcgc tgacgatgtg cacgcgatgg cccagcggtt cgagcgcgtc
     5101 ctgcaggcgc tgccgcaggg ccgccaggcc gccgaaatag cgcaggctgg cctgcacctc
     5161 gagcagcacg gtgtggggcg tgtcgtcggg ggtcgcggcg ctgggcggct ggatcgagac
     5221 gccgggcgtg aaggccagcg ccgcgtgcgc gacggcggcc agggcctgcg cgtcacgctc
     5281 gcggtcggcc tgcccgaacg tgagctgcgg cgccagcgcc agcgcggtcg cgcgcttgaa
     5341 gccgggtctg acaccgagcg cgcgcgccgc cgcgttgacg tgcaggatcg catgcgcatc
     5401 catcagcgcc agcggccgcg cccccggcgc ggacggcagc gtggccgcga acgattcgag
     5461 cgacagcagc ggcaggtgca gggcgatcca gagcacgacg aaggcgatcc acggggacga
     5521 tgtgcgcgac gcgccggttc ggcgaacgtt cggctgctgg cgacgcgctc aaccggccgc
     5581 gaggttgacg aaggtcgcgg cgcgcaggct gcctggcggt ggcgttggaa cgtgcccgag
     5641 cgccgggcct tcgatgccgg cctgcgcgcg ctgccgcgct gcctcggaca gcaccggcgg
     5701 caaggcaagc tgcaacgctg cttccagcgg cgggccgcgg cgcttcagca cacgcaccgc
     5761 gagcgcatcg gcgccggccg ggcgcagcgc caggcgcagc ggcgccgccg tcgggcgctg
     5821 cgccgcgctc aactcgcaca gcacgaaggc cgg
//
LOCUS       DATMJB010000180         7232 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_118091, whole genome shotgun sequence.
ACCESSION   DATMJB010000180 DATMJB010000000
VERSION     DATMJB010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118091"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="VNU48_04025"
     CDS             complement(<1..1462)
                     /locus_tag="VNU48_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWI10475.1"
                     /translation="MTELPAPAPRPASWAALAWRQLLRDLRAGELRLLVVAVTLAVAA
                     LSAVGFFADRIEGGLARDARQLLGGDAIVSSDRPTPAAFAAKARELGLTTATTAGFPS
                     MGRAGEAQGGASRLVSVKAVSAGYPLRGQVTLAGAPGSPEIRRTGAPERGSAWVDPAL
                     LVALNLKVGDTLLLGDTGLRIAQTIVNEPDRGTGFASFAPRVILNEADLPATGLVQPA
                     SRVSYRLAVAATGASEQPVQQFVEWAEAQVKAANIRGLRIESLATGRPEMRQTLDRAS
                     KFLNLVALLAALLAAVAVGIASRDFASRHLDDCAMLRVLGLPQRRIALQYIAEFGAVG
                     LIASLAGVLLGFAVHYVFVLLLAGLVEASLPAPSLWPALFGVGVGFTLLMGFGLPPVL
                     QLARVPPLRVIRRDVGALKPASIAVLGAGAMGFVALLLAVSSDLKLGLIAVGGFAGAA
                     LFFALLSWLAVIVLKRAVPESRAPRWLVLATRQIAAR"
     gene            1577..2449
                     /locus_tag="VNU48_04030"
     CDS             1577..2449
                     /locus_tag="VNU48_04030"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HWI10476.1"
                     /translation="MKNTAALPPIFVSHGSPMIALEPGAAGAFMRTLGWGLDAEFGRP
                     KAILAISAHTAARAPVLLAAARHEAVYDFGNFDPRLNQLRYDAPGAPELATRVAALLE
                     AAQLAVHTLPEGGLDHGIWTALRYVYPSADVPILPLAFVPSQSPAQQFALGAALSALT
                     LEGVLVLGTGSITHNLQRVFAGGLRAAEGRAEIPESAAFREWLRARAAALDWDALFDY
                     RRRAPHAVDMHPTDEHLLPWYVAAGAGGRTDAPVRLHASVELGSLGMDAYAFGPNAQR
                     LARRLEAARAHVAP"
     gene            2463..3077
                     /locus_tag="VNU48_04035"
     CDS             2463..3077
                     /locus_tag="VNU48_04035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI10477.1"
                     /translation="MENATPTLVVAAPLLLTARLRLVAADEPLAGALADFHERNRAHL
                     APWDPPTEPGFFTAAVQARRLRDAAAAFAAGTCFRYLLQPIGDPSRVIGSIDFSNVVR
                     SAFQSCHLGYALDQEYEGQALMTEALRCAIGEMFSPRINLHRIQAVYRPENWRSAEVL
                     KRLNFQDEGVCPDYLFIDGLWRVHRLVALLNRGFVVPAAWQTTA"
     gene            complement(3090..4193)
                     /locus_tag="VNU48_04040"
     CDS             complement(3090..4193)
                     /locus_tag="VNU48_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWI10478.1"
                     /translation="MFKNLARASVSTLAAILALAAPVIHAQAPSAPPKVAFVYVSPIG
                     EAGWTFQHEQGRLAMQRALGGSVTSTVVEAVAEGADSERVIRDLAAQGNRLIFATSFG
                     YLEPTLRVAADFPDVKFEHAGGYKTAANVNTYNARYYEARYLAGLLAGKTSKSGIAGY
                     VAGFPVPEVVQGINAFAIGMREANPSAQVRVLWLNTWFDPAREREAAQTLINQGADVL
                     TNHSGSPAVPQTAQASFKTKGVRAIGYQSDMHAFAPDAQLAAITLHWGGYYTQVARAV
                     LNGTWKPQPVWGGMKDGLVQLGAIDASVPAPVRALVDMRRRAIVAGSLRPFDAPLVDN
                     EGHTRLAQGTLDDPAIATMNWFVRGVVGSVPRP"
     gene            complement(4329..5369)
                     /locus_tag="VNU48_04045"
     CDS             complement(4329..5369)
                     /locus_tag="VNU48_04045"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HWI10479.1"
                     /translation="MTDFDAIARDRLPALLCAMPKAELHIHIEGSLEPELIFALGARN
                     GVALPYANVEALRAAYAFTDLQSFLDIYYAGASVLLKEEDFFDMAMAYFKRAAADHVV
                     HAELFFDPQTHTDRGVPFATVINGLSRAVEVARRELGVSALLIMCFLRHLSEEAAFAT
                     LEQALPYRDRFVGVGLDSGERGNPPEKFARVFARCRELGLHLVAHAGEEGPPAYIWSA
                     LDVLHVERIDHGVRCVEDPALVARLAAARVPLTVCPLSNVKLCVYPTLAAHQLPALLE
                     AGLCATVNSDDPAYFGGYMNRNFTETFAALPQLTARHAHRLAANSFEASFADAAAKRR
                     WSDALDKVFADA"
     gene            complement(5464..6639)
                     /locus_tag="VNU48_04050"
     CDS             complement(5464..6639)
                     /locus_tag="VNU48_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWI10480.1"
                     /translation="MTLHSKRSLIRIAGWSSLAAAAALVGCGKKEEAPAAAAPASAAP
                     MAAASAAPAGPLKIAFAYVGPVGDGGWTFAHDNARKALEKEFGDKIVTSFVEKVPEAA
                     DAERVFRDMAGQGNTLIFGTTFGYMEPMLKVATDLKDVKFEHATGYKTAPNMRTYDSR
                     TYEGAYMAGVIAGGMTKTNTVGVVGSIPIPEVIRNINSFTLGAQSVNPKVKTKVVWVN
                     EWFNPPKETEAAQSLMNGGADVLMQNTDSSAVLQTAEKAGKYAFGWDSDMTAYAPKAH
                     LASAVINWAPYYIKATKDALGGTWKGDERAWWGVKQGAIDLVSISDKVPAELKAKAEA
                     AKAGLKDGSLVIWKGPITSNDGKEVLAKDAAADDKFLGGINFFVKGVEGKVPGSDKK"
     gene            complement(6696..>7232)
                     /locus_tag="VNU48_04055"
     CDS             complement(6696..>7232)
                     /locus_tag="VNU48_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI10481.1"
                     /translation="IPVIGPALFKQHPMVYVAIALTIALAWFLYRSRAGLVLRAVGES
                     PESAHALGYPVRRIRLLAVVVGGALCGISGAYLSVIYTPLWVEGMVAGKGWIALALTT
                     FATWRPARVLLGAYLFGGVTMLQFALQGEGVQIASQFLTMMPYLATILVLVLISRNAT
                     FIRVNMPASIGKPFFPGS"
BASE COUNT         1050 a         2605 c         2537 g         1040 t
ORIGIN      
        1 ggcgggcggc gatctggcgc gtcgccagca ccagccagcg cggcgcgcgc gactcgggca
       61 ccgcgcgctt cagcacgatc accgccagcc agctgagcag cgcgaagaac agcgccgcgc
      121 cggcaaagcc gccgaccgcg atcagcccca gcttcaggtc cgacgagacc gccagcagca
      181 gcgccacgaa acccatcgca ccggcgccga gcacggcgat cgaggccggc ttcagcgcgc
      241 ccacatcgcg ccggatcacg cgcagcggcg gcacgcgcgc cagctgcagc accggcggca
      301 ggccgaagcc catcagcagc gtgaagccga cgccgacgcc gaacagcgcc ggccacaggc
      361 tgggcgccgg caggctcgct tcgacgagcc cggccagcag cagcacgaac acgtagtgca
      421 ccgcgaagcc gagcagcacg ccggccaggc tcgcgatcag cccgaccgcg ccgaactcgg
      481 cgatgtactg cagcgcgatg cggcgctgcg gcaggccgag cacgcgcagc atcgcgcagt
      541 cgtcgaggtg ccggctcgcg aaatcgcgcg acgcgatgcc caccgccacc gccgccagca
      601 gcgccgcgag cagcgccacc aggttcagga acttcgacgc gcggtccagc gtctggcgca
      661 tctcgggccg gccggtggcg agcgactcga tgcgcaggcc gcggatgttc gcagccttga
      721 cctgggcctc ggcccactcg acgaactgct gcaccggctg ctcgctcgcg ccggtggccg
      781 cgaccgcgag ccggtagctg acgcggctgg ccggctggac caggccggtc gcgggcaggt
      841 cggcctcgtt caggatcacg cgcggcgcga acgacgcgaa gccggtgccg cggtcgggct
      901 cgttgacgat ggtctgcgcg atgcgcaggc cggtgtcgcc gagcagcagc gtgtcaccga
      961 ccttcaggtt cagcgcgacc agcagcgccg ggtcgaccca ggcgctgccg cgctcgggtg
     1021 ctccggtgcg ccggatctcc gggctgcccg gcgcgccggc gagcgtcacc tggccgcgca
     1081 gcgggtagcc ggcgctgacc gccttgacac tgaccagccg cgacgcgccg ccctgcgcct
     1141 cgccggcgcg gcccatgctc gggaagccgg cggtcgtcgc ggtggtcagg ccgagctcgc
     1201 gggccttggc ggcgaacgcg gcgggtgtcg gtcgatccga cgacacgatc gcgtcgccgc
     1261 cgagcaactg gcgggcgtcg cgcgccagcc cgccctcgat tcgatcggcg aagaagccga
     1321 ccgcgctcag cgccgccacc gcgagcgtga cggcgaccac cagcaggcgc agttcgccgg
     1381 cgcgcaagtc gcgcagcagc tggcgccagg ccagcgcggc ccacgacgcg gggcgtggcg
     1441 ccggtgcagg gagttcggtc atcgggcgga acttacacga gtccgcgccg ctcccatgcg
     1501 cccaaggcct ttgcgttgaa tgctgcgtgc aagcgcctcg aacgcgcgcc gcacaccgcg
     1561 atgctgtaat cctcgcatga agaacactgc cgccctgcct ccgatcttcg tctcccacgg
     1621 ctcaccgatg atcgcgctgg agcccggcgc tgccggcgcc ttcatgcgca cgctcggctg
     1681 gggcctcgac gccgaattcg gccggccgaa ggcgatcctg gcgatctcgg cgcacaccgc
     1741 ggcgcgcgcg ccggtgctgc tggccgccgc gcgccacgag gcggtctacg acttcggcaa
     1801 cttcgatccg cgcctgaacc agctgcgcta cgacgcgccc ggcgcacccg aactcgccac
     1861 gcgcgtcgcg gcgctgctcg aggccgcgca gctcgcggtg cacacgctgc ccgagggcgg
     1921 cctggaccac ggcatctgga ccgcgctgcg ctatgtctac ccgagcgccg acgtgccgat
     1981 cctgccgctg gccttcgtgc cgagccagag cccggcgcag cagttcgcgc tcggcgcggc
     2041 gctcagcgcc ctgaccctcg aaggcgtgct ggtgctcggc accggcagca tcacgcacaa
     2101 cctgcagcgc gtgttcgccg gcgggctgcg cgcggccgag gggcgcgccg agatccccga
     2161 gagcgcggcg ttccgcgaat ggctgcgcgc gcgcgccgcg gcgctcgact gggacgcgct
     2221 gttcgactac cgccggcgtg cgccgcacgc ggtcgacatg cacccgaccg acgagcacct
     2281 gctgccgtgg tacgtggccg ccggcgcggg cgggcgcacc gacgcgccgg tgcggctgca
     2341 cgcgagcgtc gagctcggca gcctggggat ggacgcctac gccttcggcc cgaacgcgca
     2401 acgcctggcg cgccgcctcg aggcggcgcg cgctcacgtc gcgccctgaa gcgctcgccc
     2461 ggatggagaa cgccaccccg accctcgtcg tcgccgcgcc gctcctgctg accgcgcggc
     2521 tgcggctggt cgcggccgac gagccgctgg ccggtgcgct ggccgacttc cacgaacgca
     2581 accgcgccca cctcgcgccc tgggacccgc cgaccgagcc gggcttcttc accgcggcgg
     2641 tgcaggcgcg gcgcctgcgc gacgccgcgg cggcgttcgc ggccggcacc tgctttcgct
     2701 acctgctgca gccgatcggc gacccgtcgc gggtgatcgg cagcatcgac ttctcgaacg
     2761 tcgtgcgcag tgcgttccag agctgccacc tcggctacgc actcgaccag gaatacgaag
     2821 gccaggccct gatgaccgag gcgctgcgct gcgcgatcgg cgagatgttc tcgccgcgca
     2881 tcaacctgca ccgcatccag gccgtctacc ggcccgagaa ctggcgcagc gccgaggtgc
     2941 tgaagcgcct gaacttccag gacgagggcg tgtgcccgga ctacctgttc atcgacggcc
     3001 tgtggcgggt gcaccggctg gtggcgctgc tgaacagggg cttcgtcgtg cccgcggcct
     3061 ggcagacgac cgcctgagcg agcgcgcggt cagggcctcg gcaccgatcc cacgaccccg
     3121 cgcacgaacc agttcatcgt cgcgatcgcg gggtcgtcca gcgtgccctg cgccaggcgt
     3181 gtgtgccctt cgttgtcgac cagcggcgcg tcgaagggtc gcaggctgcc ggcgacgatc
     3241 gcccggcggc gcatgtcgac cagcgcgcgc accggcgccg gcacgctcgc gtcgatcgcg
     3301 ccgagctgca ccagcccgtc cttcatgccg ccccacaccg gctgcggctt ccaggtgccg
     3361 ttcagcaccg cgcgcgcgac ctgcgtgtag tagccgcccc agtgcaaggt gatcgcggcg
     3421 agctgtgcat cgggtgcgaa tgcgtgcatg tcgctctggt agccgatcgc gcgcacgcct
     3481 ttcgtcttga agctggcctg cgccgtctgc ggcaccgcgg gcgaaccgct gtggttggtc
     3541 agcacgtcgg cgccctggtt gatcagcgtc tgcgcggcct cgcgctcgcg cgccgggtcg
     3601 aaccaggtgt tcagccacag cacccgcacc tgcgcgctcg ggttggcctc gcgcatgccg
     3661 atcgcgaacg cgttgatgcc ctgcacgacc tcgggcaccg ggaagccagc aacatagccg
     3721 gcgatcccgc tcttgctggt cttgccggcg agcagcccgg ccaggtagcg cgcctcgtag
     3781 tagcgcgcgt tgtaggtgtt gacgttggcc gcggtcttgt agccgccggc gtgctcgaac
     3841 ttcacgtcgg gaaagtcggc cgcgacgcgc aaggtcggct cgaggtagcc gaagctggtc
     3901 gcgaagatca gccggttgcc ctgcgcggcg agatcgcgga tcacgcgctc cgagtcggca
     3961 ccttcggcga ccgcttcgac cacggtgctc gtgacgctgc cgccgagcgc gcgctgcatc
     4021 gccaggcgcc cctgctcgtg ctggaaggtc cagccggcct cgccgatcgg gctgacgtag
     4081 acgaaggcga ccttcggcgg cgccgacggc gcctgggcgt gaatcacggg tgccgcaagg
     4141 gcgaggatcg cagccagcgt ggacacgctg gcccgggcga ggtttttgaa catggtgttc
     4201 ctctacatgg ccccgattcg gcgaacgagc cggccgcggg ggcacgcggc gggtctcgcg
     4261 ccgattgtcg cagagcctcg ccgcaccacg ggtgcgttcc agtgaccgcg tgcgacggtc
     4321 cggtcgcttc aggcgtcggc gaagaccttg tcgagcgcgt cggaccagcg gcgcttcgcg
     4381 gcagcgtcgg cgaagctcgc ctcgaagctg ttggcggcga gccggtgcgc gtgccgcgcc
     4441 gtcaactgcg gcagcgccgc gaaggtctcg gtgaagttgc gattcatgta gccgccgaaa
     4501 taggccgggt cgtcggagtt gacggtcgcg cacaggccgg cctcgagcag cgcgggcaac
     4561 tggtgcgcgg ccagcgtcgg gtagacgcac agcttgacgt tcgacagcgg gcacaccgtc
     4621 agcggcacgc gagcggctgc gaggcgcgcc accagcgccg ggtcttcgac gcagcgcacg
     4681 ccgtggtcga tgcgctcgac atgcagcacg tcgagcgcgc tccagatgta ggccggcggc
     4741 ccttcctcgc cggcatgcgc gaccaggtgc aggccgagct cgcggcagcg tgcgaacacg
     4801 cgcgcgaact tctccggcgg gttgccgcgc tcgccgctgt ccaggccgac gccgacgaag
     4861 cggtcgcgat acggcagcgc ctgctcgagc gtcgcgaacg cggcttcctc gctcagatgc
     4921 cgcaggaagc acatgatgag cagcgcgctc acgccgagtt cgcgccgcgc gacctcgacg
     4981 gcgcgcgaca gcccattgat cacggtggcg aacggcacgc cacggtcggt gtgggtctgc
     5041 gggtcgaaga agagttcggc gtggacgacg tggtcggccg cggcgcgctt gaaatacgcc
     5101 atggccatgt cgaagaaatc ctcttccttc aacagcacac tcgcgccggc gtagtagatg
     5161 tcgaggaagc tctgcaggtc ggtgaacgca taggccgcgc gcagcgcctc gacgttcgcg
     5221 tacggcagcg cgacgccatt gcgcgcgccg agcgcgaaga tcagttcggg ctcgagcgag
     5281 ccctcgatgt ggatgtgcag ctcggccttg ggcatcgcgc acaggagtgc gggcaggcgg
     5341 tcgcgggcga tggcgtcgaa gtcggtcatg actgtcccga gagaaacgaa gaggccgcca
     5401 gggtcacgaa ggggctcctt cgtggccctc ggcggcctca tcatcatacg aactgagcgc
     5461 ggactacttc ttgtcgctgc ccggcacctt gccttcgacg cccttgacga agaagttgat
     5521 gccgccgagg aacttgtcgt cggccgcggc gtccttggcc agcacttcct tgccgtcgtt
     5581 gctcgtgatc gggcccttcc agatcaccag gctgccgtcc ttcaggccgg ccttcgcggc
     5641 ctcggccttg gccttcagct cggccggcac cttgtcggag atcgagacca ggtcgatcgc
     5701 gccttgcttg acgccccacc acgcgcgctc gtcacccttc caggtgccgc ccagcgcgtc
     5761 cttggtggcc ttgatgtagt acggggccca gttgatcacg gccgacgcga ggtgcgcctt
     5821 cggtgcgtag gcggtcatgt cgctgtccca gccgaacgcg tacttgccgg ccttctcggc
     5881 ggtctgcagc accgcgctcg aatcggtgtt ctgcatcagc acgtcggcgc cgccgttcat
     5941 cagcgactgc gcggcctcgg tctccttcgg cgggttgaac cactcgttga cccagaccac
     6001 cttggtcttg accttggggt tcaccgactg cgcgccgagc gtgaagctgt tgatgttgcg
     6061 gatcacttcc gggatcggga tcgagccgac cacgccgacg gtgttggtct tggtcatgcc
     6121 gccggcgatc acgccggcca tgtacgcgcc ctcgtaggta cgcgaatcgt aggtgcgcat
     6181 gttcggcgcg gtcttgtagc cggtggcgtg ctcgaacttc acgtccttca ggtcggtcgc
     6241 gaccttcagc atcggctcca tgtagccgaa ggtggtgccg aagatcaggg tgttgccctg
     6301 gccggccatg tcgcggaaca cgcgctcggc gtcggccgcc tcgggcacct tctcgacgaa
     6361 gctggtgacg atcttgtcgc cgaattcctt ctcgagcgcc ttgcgcgcgt tgtcgtgcgc
     6421 gaaggtccac ccgccgtcgc cgaccgggcc gacataggcg aacgcgatct tcaacgggcc
     6481 ggccggcgcg gccgaggcag ccgccatcgg tgcggccgat gcgggtgccg cagcggcggg
     6541 cgcctcttcc ttcttgccgc agcccacgag cgcggcggcc gccgcgagag agctccagcc
     6601 ggctattctg atcaatgaac gcttggaatg cagggtcatg tcttctccag gttgaacgcg
     6661 ggtgggggtg cggtgcagga attatgtgac aggccctagc tgccggggaa gaacggcttg
     6721 ccgatcgagg ccggcatgtt gacgcggatg aaggtcgcgt tgcgcgagat cagcaccagc
     6781 accaggatgg tcgcgagata cggcatcatc gtcaggaact ggctggcgat ctgcacgccc
     6841 tcgccctgca gcgcgaactg cagcatcgtg acgccgccga acaggtaggc accgagcagc
     6901 acgcgcgccg gtcgccaggt cgcgaaggtc gtcagcgcca gcgcgatcca gcccttgccc
     6961 gccaccatgc cctcgaccca cagcggcgtg tagatcaccg acaggtacgc gcccgagatg
     7021 ccgcacagcg cgccgccgac cacgaccgcg agcagccgga tgcgccgcac cggataaccc
     7081 agcgcatgcg ccgactcggg cgattcgccg accgcgcgca gcaccagccc ggcgcgcgag
     7141 cggtacagga accaggcgag cgcgatcgtc aacgcgatcg cgacgtacac catcgggtgc
     7201 tgcttgaaga gcgccgggcc gatcaccgga at
//
LOCUS       DATMJB010000181         4543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_781910, whole genome shotgun sequence.
ACCESSION   DATMJB010000181 DATMJB010000000
VERSION     DATMJB010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781910"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3626
                     /gene="hrpA"
                     /locus_tag="VNU48_04060"
     CDS             <1..3626
                     /gene="hrpA"
                     /locus_tag="VNU48_04060"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HWI10482.1"
                     /translation="GERGLIGHTQPRRIAARAVATRIAHELGQPVGEAVGYKVRFTDH
                     TRPDAYIKLMTDGILLAETQTDPLLHAYDTIIIDEAHERSLNIDFLLGHLRQILPRRP
                     DLKVIVTSATIDADRFARHFAAGDVPAPVIQVSGRLFPVEQRWRPFEESREFDLNDAI
                     GAAVDELWREGAGDVLVFLPGEREIREAADHLRKHHPPGVEVVPLFARLSQQEQDHIF
                     EPHSARRVVLATNVAETSLTVPGIQFVVDAGTARVKRYSYRSKVEQLLVETISQAAAN
                     QRAGRCGRVSDGICIRLYDEKDFAGRPPFSDPEILRSSLAGVILRMKSLSLGTVEDFP
                     FLEPPPKRAIADGYQLLAELGAVDEFNELTPIGVELARLPLDPRVGRMILEARTRDAL
                     TEVLIIASAFSVQEVRDRPLEHQQAADAAHKKFDDEKSEFIGDLKLWKWLEDARGGQG
                     EHKVSQRKQEQLLRDNFISPRRVREWRDIHSQLHTVVAEHKWRLNGSPATYEQIHLSM
                     LAGLLGNIGCKSEDEDWYLGARGIRFYKHPGANLTKKPGRWIVAAELVETTRLFGRSI
                     AAIEPQWLERIAGHVIKTQLLEPHWEKKAAEVIALERATLYGIVIYSNRRVNFGNVDA
                     AAAREIFIREALVAGEWETKLPFVNANRKLIAQVEELEHKSRRQDVLVDDELIHAFYD
                     AQLPADVCSGHSLERWYRDEVKRQPRLLMLTREELMRHEAAGITSSAFPKTIRLGGID
                     CAASYLHAPGDAKDGVTVTVPIYALNQVGEDRCEWLVPGMLKDKVLALVKSLHQRPRS
                     RLVPLPEYAQAFVEAVEFGHGSLLDALLKSVRDKTQLDIKRADFKLEQLPPHLLMNFR
                     INDEHGRQLGTGRHLAALKAELGGQARSAFQALAALKLDAAAAAPVPQANNVAVIVKP
                     ARAAATSVAAEKYTSWTFGELPELMEIRQAGQSLIGFPALIDKDAHVEIEVFDEPEVA
                     ARKHRAGLRRLIALQIRDALKYLEKNIPDLQKMSVAYLLVGNGSADELREQIIDVALD
                     RAFLADALPTDAAGFARCVEGGRGRLTLIANEVARSAGAILIEHSAALRKLKDARAPK
                     EVADDIAAQLARLVTKRFVAATPWAQLAHLPRYLRAIVMRLDKLRSDPARDAQRLAEL
                     RPIEQRYWRHLAERKGVADARLDEFRWLLEELRVSLFAQELRTPQPVSVKRLEKVWAQ
                     ITA"
     gene            3754..4011
                     /locus_tag="VNU48_04065"
     CDS             3754..4011
                     /locus_tag="VNU48_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWI10483.1"
                     /translation="MHYVWMAVIGFIVGLVARAILPGTQSLGIILTAVLGIAGSFLAE
                     FVGRAMGWYAEGANAGFIASVIGAIVLLFVVAKLKGPSGTS"
     gene            4019..>4543
                     /locus_tag="VNU48_04070"
     CDS             4019..>4543
                     /locus_tag="VNU48_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10484.1"
                     /translation="MPTKAVPAGPSDWVVVPGTATQIEAVARQCRRLVTKRALLAAGV
                     AIVPIPGVDWVTDIAVLMKLIPEISHAFGLTPEQVERLAPDRRLVVYKAISAGGGLLV
                     GRLVTRELIVRLLRLVGVRLTTQQAAKYVPIAGQAVSAALTFSSLKFVCEQHIQQCVA
                     VSRQLMLPAPAAATA"
BASE COUNT          756 a         1552 c         1590 g          645 t
ORIGIN      
        1 gcggtgagcg cgggctgatc gggcatacgc agccacggcg aattgccgcg cgtgcggtgg
       61 ccacgcgcat cgcgcacgag ctcgggcaac cggtcggcga ggcggtcggt tacaaggtgc
      121 gcttcaccga ccacacgcgc ccggacgcgt acatcaagct gatgaccgac ggcatcctgc
      181 tcgccgagac ccagaccgac ccgctgctgc acgcctacga cacgatcatc atcgacgagg
      241 cgcacgagcg cagcctgaac atcgacttcc tgctcgggca cctgcggcag atcctgccgc
      301 gccggcccga tctgaaggtc atcgtcacgt cggcgacgat cgacgccgat cgcttcgcgc
      361 gccacttcgc ggcgggcgac gtgccggcgc cggtcattca agtctcgggc cgcctgttcc
      421 cggtcgagca gcgctggcgc ccgttcgagg agagccgcga gttcgatctg aacgacgcga
      481 tcggcgcggc ggtcgacgag ctctggcgcg aaggcgcggg cgacgtgctc gtgttcctgc
      541 ccggcgaacg cgagatccgc gaggccgccg atcatctgcg caagcaccat ccacccggcg
      601 tggaagtcgt gccgctgttc gcacgcttaa gccagcagga gcaggaccac atcttcgagc
      661 cgcacagcgc gcgccgagtc gtgctcgcga cgaacgtcgc cgagacctcg ctgacggtgc
      721 cgggcatcca gttcgtcgtc gacgccggca ccgcgcgcgt caagcgctac agctaccgca
      781 gcaaggtcga gcagctgctg gtcgagacta tcagccaggc cgcggcgaac cagcgcgccg
      841 ggcgctgcgg gcgcgtcagt gacggcatct gcatccgcct ctacgacgag aaggacttcg
      901 ccgggcggcc gccgttcagc gaccccgaga tcctgcgctc gtcgctggcc ggcgtgatcc
      961 tgcgcatgaa gtcgctgagc ctgggcaccg tcgaggactt cccgttcctc gagccgccgc
     1021 ccaagcgcgc gatcgccgac ggctaccagc tgctggccga gctcggggcg gtcgacgagt
     1081 tcaacgagct gacgcccatc ggcgtcgaac tcgcgcgcct gccgctggac ccgcgcgtcg
     1141 gccgcatgat cctggaagcg cgaacccgcg acgcactgac cgaggtgttg atcatcgcca
     1201 gcgcgttcag cgtgcaggag gtgcgcgacc ggcccctcga acaccagcag gcggccgacg
     1261 cggcgcacaa gaagttcgac gacgagaaga gcgagttcat cggcgacctg aagctgtgga
     1321 agtggctgga ggacgcgaga ggcgggcagg gtgaacataa ggtgtcgcaa cgcaagcagg
     1381 aacagctgct gcgcgacaac ttcatctcgc cgcgccgcgt gcgcgagtgg cgcgacatcc
     1441 actcgcagct gcacaccgtc gtcgccgaac acaagtggcg gctcaacgga tcgccggcga
     1501 cctacgaaca gatccacctg tcgatgctcg ccgggctgct cggcaacatc ggctgcaaga
     1561 gcgaggacga ggactggtac ctgggcgcgc gcggcatccg cttctacaag cacccgggcg
     1621 cgaacctgac caagaagccg gggcgctgga tcgtcgccgc cgaactcgtc gagaccacgc
     1681 gcctgttcgg ccgcagcatc gcggcgatcg agccgcaatg gctggagcgc atcgccggtc
     1741 acgtcatcaa gacccagctt ctcgagccgc actgggagaa gaaggccgcg gaagtgatcg
     1801 cgctggagcg tgccactctc tacggcatcg tcatctacag caaccggcgc gtgaacttcg
     1861 gcaacgtcga cgcagccgcg gcgcgcgaga tcttcatccg cgaggccttg gtcgccggcg
     1921 agtgggagac caagctgccc ttcgtgaacg cgaaccgcaa gctgatcgcg caggtcgagg
     1981 agctcgagca caagtcgcgg cgccaggacg tgctggtcga cgacgagctg atccacgcgt
     2041 tctacgacgc gcagctgccc gccgacgtgt gcagcggcca cagcctggag cgctggtacc
     2101 gcgacgaggt gaagcgccag ccgcgcctgc tgatgctgac gcgcgaggag ctgatgcgcc
     2161 acgaggcggc cggcatcacc agcagcgcgt tcccgaagac gatccggctc ggcggcatcg
     2221 actgcgccgc gagctacctg cacgcgcccg gcgacgcgaa ggacggcgtc accgtgacgg
     2281 tgccgatcta tgcgctgaac caggtcggcg aagaccgctg cgagtggctg gtgccgggca
     2341 tgctgaagga caaggtgctc gcgctcgtca agagcctgca ccagcgcccg cgctcgcgcc
     2401 tggtgccgtt gcccgagtac gcgcaggcct tcgtcgaggc ggtcgaattc ggccacggca
     2461 gcctgctcga cgcgctgctg aaaagcgtgc gcgacaagac gcagctcgac atcaagcgcg
     2521 ccgacttcaa gctcgagcag ctgccgccgc acctgctgat gaacttccgc atcaacgacg
     2581 agcacggccg ccagctcggc acaggccgcc acctcgcggc gctgaaagcc gagctcggcg
     2641 gccaggcgcg ttcggcgttc caggcactgg ccgcgctgaa gctcgatgcg gcggccgccg
     2701 cgcccgtgcc gcaggcgaac aacgtcgccg tgatcgtcaa gccggcgcgg gccgcggcga
     2761 ccagcgtcgc ggccgagaag tacacgagct ggaccttcgg cgagctgccc gagctgatgg
     2821 agatccgcca ggccggccag tcgctgatcg gctttccggc gctgatcgac aaggacgcgc
     2881 atgtcgagat cgaagtcttc gacgagcccg aggtcgcggc gcgcaagcac cgcgcgggcc
     2941 tgcgccggct gatcgcgctg cagatccgcg acgcgttgaa atacctcgag aagaacatcc
     3001 ccgacctgca gaagatgagc gtggcctacc tgctggtggg caacggcagc gccgacgaac
     3061 tgcgcgagca gatcatcgac gtggcgctcg atcgcgcgtt cctggccgat gcgctgccca
     3121 ccgacgcggc gggctttgcg cgctgcgtcg aaggcgggcg agggcgcctg accctgatcg
     3181 ccaacgaggt ggcgcgcagt gccggcgcga tcctgatcga acacagcgcg gcgctgcgca
     3241 agctgaagga tgcgcgcgca ccgaaggagg tcgccgacga catcgccgcg cagctcgcgc
     3301 gcctggtgac caagcgcttc gtcgccgcca cgccctgggc ccagctggcg cacctgccgc
     3361 gctatctgcg cgcgatcgtg atgcggctgg acaagctgcg cagcgacccg gcgcgcgacg
     3421 cgcagcgcct ggccgaactg cgcccgatcg agcagcgcta ctggcgccac ctggccgagc
     3481 gcaagggcgt ggccgatgcg cgcctcgatg aattccgctg gctgctcgag gagctgcgcg
     3541 tcagcctgtt cgcgcaggag ctgcgcacgc cgcagccggt cagcgtgaag cggctcgaga
     3601 aggtctgggc ccagatcacc gcctgacgaa cgcgggcgct gccgtaaggc gcttcgtcgt
     3661 ccgcgatgca tgcgctacat tggcgcagcg ttcgacaggc ctgcgcaaga agcgcacggg
     3721 ggccgaggcg cacccaccca caggagtcga aacatgcact acgtctggat ggcagtgatc
     3781 gggttcatcg tcggtctggt ggcgcgcgcg atcctgccgg ggacccagag cctggggatc
     3841 atcctgacgg cggtgctcgg catcgccggc tcgttcctcg cggaattcgt cggtcgggcg
     3901 atgggctggt acgccgaagg tgcgaacgct ggcttcatcg cctcggtgat cggggccatt
     3961 gtgctgttgt tcgtcgtcgc caagttgaag gggccctcgg gcacgagttg aatcggccat
     4021 gccgacgaag gcggtccctg ccggcccgag cgactgggtc gtcgtgcctg gcacggcgac
     4081 gcagatcgag gcggtcgcgc gccagtgccg acgcctggtc acgaagcgcg cgttgctggc
     4141 cgcgggtgtg gccatcgtgc cgattcccgg tgtcgactgg gtcaccgaca tcgcggtgct
     4201 gatgaagctg attcccgaga tcagccacgc gttcgggctg acgccggagc aggtcgagcg
     4261 gctggcgccg gaccggcgcc tggtggtcta caaggccatt tcggcgggcg gcgggttatt
     4321 ggtcgggcgg ctcgtgacgc gcgagctgat cgtgcggctg ctcagactcg tcggcgtgcg
     4381 gctgaccaca cagcaggcgg ccaagtacgt gccgatcgcc ggccaggcgg tctcggccgc
     4441 gctgaccttc tcgtcgttga agttcgtttg cgaacagcac attcagcagt gcgtcgcggt
     4501 gtcgcggcag ctgatgctgc cggcacccgc ggccgccacg gcg
//
LOCUS       DATMJB010000182         3466 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_291293, whole genome shotgun sequence.
ACCESSION   DATMJB010000182 DATMJB010000000
VERSION     DATMJB010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3466
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291293"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1150
                     /gene="egtD"
                     /locus_tag="VNU48_04075"
     CDS             188..1150
                     /gene="egtD"
                     /locus_tag="VNU48_04075"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HWI10485.1"
                     /translation="MTPLRTPRFIQFHVTDAAAERADIEAGLLAEPARAAPKFFYDRL
                     GSHLFEAITELPEYYPTRTEAAIFAAHGAAIASRIGAGATLIDLGAGNCAKASKLFPL
                     LEPKRYVAVDISVDFLHDALKRVQREHPALDVVGLGQDFSAALALPDDLIDGRAVFFY
                     PGSSIGNFAAGDALAFLRRIRAQADGGGLLIGVDLVKDSATLQAAYDDALGVTAAFNL
                     NLLRQLNRLIGSDFRPEDWRHIGLYNEAESRIEMHLEARRDLLVRWPGAQRAFRVGER
                     LHTENSCKYTLERFSALLRDAGFAPPVHWTDGRGWFGVFWAPAS"
     gene            complement(1210..>3466)
                     /gene="alaS"
                     /locus_tag="VNU48_04080"
     CDS             complement(1210..>3466)
                     /gene="alaS"
                     /locus_tag="VNU48_04080"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWI10486.1"
                     /translation="YAEDDEAFNIWTKEIGLAPERVIRIGDNKGARYASDNFWMMGDT
                     GPCGPCTEIFYDHGPEIAGGPPGSPDEDGDRYIEIWNNVFMQFNRDEAGVMHKLPKPS
                     VDTGMGLERLAAVLQHVHSNYEIDTFVNLLAAAKRVVDAAGAGDCDIESPSLKVIADH
                     IRACTFTVVDGVIPSNAGRGYVLRRIARRAIRHGYKLGARQPFFHALVPALAAEMGDA
                     YPELRRDEKRALEVLKQEEEGFFRTIANGMEILEKALASLDKAGTKQLDGDTAFKLHD
                     TFGFPVDLTADVCRERGVTVDEARFNVLLDEQKKRAREAGKFKMAQGLEYGGAATQFH
                     GYDELVREGVRVTAVYVDGSAVASAKAGDDAVIVLDHTPFYAEAGGQVGDTGELRNAS
                     SRVIVDDTIKVQAAVFGHHGRVVEGTVAVGDVFSARVDAEKRLRTVRNHSATHLMHKA
                     LREVLGEHVQQKGSLVTPERTRFDFAHNAPMTEAQIARVEALVNAEVLANAATQARVM
                     PIDAAQKSGAMMLFGEKYGDEVRVLDIGSSRELCGGTHVQRTGDIGLFKVVGESGVAA
                     GVRRVEAITGDNALAYLQSLEGTVQQLAGALKAAPAEVPARLIQVLDQVRALEKELAA
                     LKGKLASSQGDELIASAVDVNGIKVLAAMLPGADAKALRETMDKLKDKLKTAAIVLAS
                     VDGGKVQIAAGITNDSLGKLKAGELVNFVAQQVGGKGGGKADMAMAGGTEPGKLDAAL
                     ASVRGWVAERL"
BASE COUNT          467 a         1270 c         1156 g          573 t
ORIGIN      
        1 acgcgccgcg ccaggccagg cgaacgccgc ggccgcggcc agcgcggcgg cgagcgcctg
       61 acggcgcgtt gtcctaccgc agcggtgggc gagcaagctc tgcattcgga gtccagccgg
      121 tgtaaggtcg ggcagcgttc atcgacaccc tcggacctgg tcggccacga ttgcaccaca
      181 agctgccatg accccattgc gcacgccccg attcatccag ttccatgtga ccgacgcggc
      241 cgccgagcgc gccgacatcg aagccggcct gctcgccgag ccggcgcgtg ccgcccccaa
      301 gttcttctac gaccggctcg gctcgcacct gttcgaggcc atcaccgagc tgcccgagta
      361 ctacccgacg cgcaccgagg cggcgatctt cgccgcgcac ggcgccgcca tcgcgagccg
      421 gatcggtgcc ggcgcgacgc tgatcgacct gggcgccggc aattgcgcga aggcctcgaa
      481 actgttcccg ctgctcgagc cgaagcgcta cgtggcggtc gacatctcgg tcgacttcct
      541 gcacgacgcg ctcaagcgcg tgcagcgcga gcacccggcg ctcgatgtgg tcggcctcgg
      601 gcaggacttc tcggccgcgc ttgcgctgcc cgacgacctg atcgacggcc gcgcggtgtt
      661 cttctacccc ggctccagca tcggcaactt cgcagcgggc gacgcgctcg cgttcctgcg
      721 gcgcatccgc gcgcaggccg acgggggcgg gctgctgatc ggcgtcgacc tggtcaagga
      781 cagcgcgacc ctgcaggccg cctacgacga cgcgctgggc gtcacggccg cgttcaacct
      841 gaacctgctg cgccagctga accggctcat cggcagcgac ttccggcccg aggactggcg
      901 ccacatcggg ctgtacaacg aggccgaatc gcgcatcgag atgcacctcg aggcacgccg
      961 cgacctgctc gtgcgctggc ccggcgcgca gcgcgcgttc cgtgtcggcg agcggctgca
     1021 caccgagaac tcgtgcaagt acacgctcga gcgtttcagc gcactgctgc gggacgccgg
     1081 cttcgcgccg cccgtgcact ggaccgacgg gcgcggctgg ttcggcgtgt tctgggcgcc
     1141 ggccagctga gggaaagcat agcccttcgc ggtgccctcg gtcagcgcgt catgcacgcg
     1201 ccctcgccat cagaggcgct cggcgaccca gccgcgcacc gacgcgagcg ccgcgtcgag
     1261 cttgcccggc tcggtgccgc cggccatcgc catgtcggcc ttgccgccgc ccttgccgcc
     1321 gacctgctgg gccacgaagt tgacgagctc gccggccttc agcttgccca ggctgtcgtt
     1381 ggtgatgccg gccgcgatct gcaccttgcc gccgtcgacg ctggcgagca cgatcgccgc
     1441 ggtcttgagc ttgtccttca gcttgtccat ggtctcgcgc aaggccttcg cgtcggcacc
     1501 cgggagcatc gcggccagca ccttgatgcc gttcacgtcg acggccgagg cgatcagctc
     1561 gtcgccctgc gacgaggcga gcttgccctt cagcgcggca agttccttct cgagcgcacg
     1621 cacttggtcc agcacctgga tcagccgcgc cggcacctcg gccggcgcgg ccttgagcgc
     1681 gccggcgagc tgctgcaccg tgccttccag cgactgcaga taggccagcg cgttgtcgcc
     1741 ggtgatcgcc tcgacgcggc gcacgccggc cgccacgccg ctttcgccga cgaccttgaa
     1801 caggccgatg tcgccggtgc gctgcacgtg cgtgccgccg cacagttcgc ggctgctgcc
     1861 gatgtcgagc acgcgcacct cgtcgccgta cttctcgccg aacagcatca tcgcgcccga
     1921 cttctgcgcc gcgtcgatgg gcatcacgcg cgcctgggtc gcggcgttcg cgagcacctc
     1981 ggcgttcacc agcgcctcga cccgggcgat ctgcgcctcg gtcatcgggg cgttgtgcgc
     2041 gaagtcgaag cgcgtgcgct caggcgtaac cagcgagccc ttctgctgca cgtgctcgcc
     2101 gagcacctcg cgcagcgcct tgtgcatcag gtgcgtggcc gaatggttgc gcaccgtcct
     2161 caatcgcttc tcggcatcga cgcgcgcgct gaacacatcg ccgaccgcga ccgtgccctc
     2221 gacgacgcgg ccgtggtggc cgaacaccgc cgcctggacc ttgatcgtgt cgtcgacgat
     2281 cacgcgcgag ctcgcgttgc gcagttcgcc ggtgtcgccg acctggccgc cggcctcggc
     2341 atagaacggc gtgtggtcga gcacgatcac cgcgtcgtcg ccggccttcg cgctcgcgac
     2401 cgcgctgccg tcgacataca ccgcggtgac cctgacgccc tcgcgcacga gctcgtcgta
     2461 gccgtggaac tgcgtggccg cgccgccgta ctccagcccc tgcgccatct tgaacttgcc
     2521 ggcctcgcgg gcgcgtttct tctgctcgtc cagcagcacg ttgaagcgcg cctcgtcgac
     2581 cgtgacgccg cgctcgcggc agacgtcggc ggtcaggtcg accgggaagc cgaaggtgtc
     2641 gtgcagcttg aacgcggtgt cgccgtcgag ctgcttcgtg cccgccttgt ccaatgaggc
     2701 gagcgccttc tcgagaatct ccatgccgtt cgcgatggtg cggaagaagc cctcctcttc
     2761 ctgcttcagc acctcgagcg cgcgcttctc gtcgcgccgc agttcggggt aggcatcgcc
     2821 catctcggcg gcgagcgccg gcaccagcgc gtggaagaac ggttggcgcg cgcccagctt
     2881 gtagccgtgg cggatcgcgc gccgcgcgat gcggcgcagc acgtagccgc ggccggcgtt
     2941 gctcgggatc acgccgtcga ccaccgtgaa ggtgcaggcg cggatgtggt cggcgatcac
     3001 cttcagcgac ggcgactcga tgtcgcagtc accggcgccg gccgcgtcga ccacacgctt
     3061 ggctgccgcc aacaggttga cgaaggtatc gatctcgtag ttgctgtgca cgtgctgcag
     3121 caccgccgcg agccgctcga ggcccatgcc ggtgtcgacg ctgggcttcg gaagcttgtg
     3181 catcacgccc gcttcgtcgc ggttgaactg catgaacacg ttgttccaga tctcgatgta
     3241 gcggtcgccg tcctcgtcgg gcgagcccgg cgggccgccg gcgatctcgg ggccgtggtc
     3301 gtagaagatc tcggtgcacg ggccgcacgg gccggtgtcg cccatcatcc agaagttgtc
     3361 ggacgcgtag cgcgcgccct tgttgtcgcc gatgcggatc acgcgctcgg gtgctaggcc
     3421 gatttccttg gtccagatgt tgaaggcttc gtcatcctct gcatac
//
LOCUS       DATMJB010000183         2153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_449581, whole genome shotgun sequence.
ACCESSION   DATMJB010000183 DATMJB010000000
VERSION     DATMJB010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449581"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VNU48_04085"
     CDS             <1..276
                     /locus_tag="VNU48_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI10487.1"
                     /translation="KGGVVGMTLPIARDLARNGVRNMTIAPGIFGTPMMFTMPKEVQE
                     SLAASVPFPSRLGTPADYAKLVHQIVTNEMLNGEVIRLDGAIRLAPK"
     gene            complement(342..1544)
                     /locus_tag="VNU48_04090"
     CDS             complement(342..1544)
                     /locus_tag="VNU48_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10488.1"
                     /translation="MNRLIVAVGATLVFGAAHGQSVRPDVGKPLQAAEALIKSGKFRE
                     ALAKVREADAVGGKTAAESTMVERMRLAAASGAGDADTAARSFDAIGSGIAGADRLRT
                     IESIAGSYYRAKDYAKAQQWYARYFKEGGTSSANRTLMIQTQYLSGDLAGASRELMAE
                     IQGAERSGAAPAEDRINLLMNAAVRQKDVNAETFALERLVTYYPKKEYWVSLLSRMQR
                     KSSFSDRLSLDAYRLSLATGSMSAAADYSEMAQLALQAGSGAEAKQVIDKGFAAGVLG
                     TGPEAERHKRLRDLVDKRIAEAKKTGTADEKEALAAKDGNELVTIGMNQVYEGQKAQG
                     VQLMQQGIAKGGLKRPEDAKLHLAIAQLVAGENAKAQATLKTVSGSDGTADLARLWAL
                     YARRAKAS"
     gene            complement(1561..1989)
                     /locus_tag="VNU48_04095"
     CDS             complement(1561..1989)
                     /locus_tag="VNU48_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWI10489.1"
                     /translation="MNVGTSSSSSGEPEAMMDINTTPLIDVMLVLIIMLIITIPVQLH
                     SVNLNMPAGNPPPPVKEPVVVTIDVDFDGTILWNGEAVPTRQALEVKLRDAAAQPDQP
                     EVHVRPNKLVEYKYVAEVLAESQRLGVTKLGMVGNEQFVK"
     gene            complement(2001..>2153)
                     /locus_tag="VNU48_04100"
     CDS             complement(2001..>2153)
                     /locus_tag="VNU48_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWI10490.1"
                     /translation="KGIAVMNPQPEIHIRGDQNARYESIGRVVFACQRAAIQKISFVT
                     EPPPKG"
BASE COUNT          290 a          766 c          695 g          402 t
ORIGIN      
        1 aagggcggcg tggtcggcat gaccctgccg atcgcgcgcg acctcgcgcg caacggtgtc
       61 cgcaacatga cgatcgcgcc tggcatcttc ggcacgccga tgatgttcac gatgcccaag
      121 gaggtacagg aatcgctggc cgcgagcgtg ccgttcccga gccgcctggg cacgcccgcc
      181 gactacgcga agctggtgca ccagatcgtc acgaacgaga tgctcaatgg cgaggtgatc
      241 cgcctcgacg gggcgatccg cctggcgccc aagtgatcag cgccgcttga gtgcaaaacg
      301 cggcccgagg gccgcgtttc ttcgtgtgcg aggccttctc ctcaggacgc cttcgcgcgc
      361 cgcgcgtaca gcgcccacag gcgcgcgaga tcggcggtgc cgtcgctgcc cgacaccgtc
      421 ttcaaggtcg cctgcgcctt cgcgttctcg ccggccacga gctgcgcgat cgccaggtgc
      481 agcttcgcgt cctcgggccg cttcagcccg cccttggcaa tgccctgctg catcagctgg
      541 acgccttgcg ccttctgacc ttcgtagacc tggttcatgc cgatcgtcac gagttcgttg
      601 ccgtccttgg ccgccagcgc ttccttctcg tccgcggtgc cggtcttctt cgcctcggcg
      661 atgcgcttgt cgaccaggtc gcgcaggcgc ttgtggcgct cggcctccgg gccggtgccg
      721 agcacgccgg cagcgaaacc cttgtcgatc acctgcttgg cctcggcgcc cgagccggcc
      781 tgcagcgcga gctgcgccat ctcggagtaa tcggcggcgg cgctcatgct gccggtcgcc
      841 agcgacaggc gataggcgtc cagcgacagc cggtccgaga agctcgactt gcgctgcatc
      901 cggctcagca ggctgaccca gtactccttc ttcgggtagt acgtcaccag gcgctcgagc
      961 gcgaaggtct cggcgttcac gtccttctgc cggaccgcgg cgttcatcag caggttgatg
     1021 cggtcctcgg ccggcgcggc gccactgcgc tcggcgccct ggatctcggc catcagttcg
     1081 cgcgacgcgc cggccaggtc gcccgacagg tattgcgtct ggatcatcag cgtgcgattg
     1141 gcgctgctgg tgccgccctc cttgaagtag cgcgcgtacc actgctgggc tttcgcgtaa
     1201 tccttggcgc ggtagtagct gccggcgatc gactcgatcg tgcgcagccg gtcggcgccg
     1261 gcgatgccgc tgccgatcgc atcgaaggag cgcgccgcgg tgtcggcgtc gccggcaccc
     1321 gaggccgctg ccaggcgcat gcgctcgacc atcgtcgatt cggccgcggt cttgccgccc
     1381 accgcatcgg cctctcgcac cttggcgagc gcctcgcgga acttgcccga cttgatcagc
     1441 gcctcggcgg cctgcaaggg cttgcccacg tcggggcgca cgctctgccc gtgcgccgcg
     1501 ccgaacacga gcgtcgcgcc gaccgcgacg atcagccgat tcatctgtct gttcatacgc
     1561 tcatttcacg aactgctcgt tgcccaccat gccgagcttg gtcacgccca ggcgctgcga
     1621 ttcggccagc acctcggcga catacttgta ctcgacgagc ttgttcggcc gcacgtgaac
     1681 ctcgggctgg tcgggctgcg ccgcggcgtc gcgcagcttc acttccagcg cctggcgcgt
     1741 cggcacggcc tcgccgttcc acaggatcgt gccgtcgaag tcgacgtcga tcgtgacgac
     1801 caccggctcc ttcaccggcg gcggcgggtt gccggccggc atgttcaggt tcaccgagtg
     1861 cagctgcacc gggatcgtga tgatcagcat gatgatcagc accagcatca cgtcgatcaa
     1921 cggcgtggta ttgatgtcca tcatcgcttc gggctcgccg gaactgctgc tgctcgtgcc
     1981 tacgttcatg gccatgctgc ttaccccttc ggcggcggtt cggtgacgaa gctgatcttc
     2041 tggatcgccg cgcgctggca cgcgaagacc acccggccga tcgactcgta gcgtgcgttc
     2101 tgatcgccgc ggatgtggat ctcgggctgc gggttcatca ccgcgatgcc ctt
//
LOCUS       DATMJB010000184         3003 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_710594, whole genome shotgun sequence.
ACCESSION   DATMJB010000184 DATMJB010000000
VERSION     DATMJB010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710594"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VNU48_04105"
     CDS             complement(<1..996)
                     /locus_tag="VNU48_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HWI10491.1"
                     /translation="MASNGTLTDVAGLRVGHFTDARRPTGCTVILTPEGAVAGVDVRG
                     AAPGTRETELLSPLNAVEAVHALLLAGGSAFGLDAAGGVMRWLDERGFGVPVGPAQGH
                     ARIGRPIRVPIVPAAILFDLWVGDPSIRPDAAAGYAACEAASDAAVAQGNVGAGAGCS
                     VGKLFGMARAMRGGLGSASVVVDGITVAALVAVNAIGDVVDPATGRVIAGARTPDGSA
                     LLGTMQALLRGELPAPLQVGAATTLGIVATDAVLTKAQANKMAQMAHDGFARAINPVH
                     TMTDGDTIFALATGAAGRTANVTLLGALAAEVTATAIVRAVRAANALPGLPAAADI"
     gene            complement(1018..2157)
                     /locus_tag="VNU48_04110"
     CDS             complement(1018..2157)
                     /locus_tag="VNU48_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI10492.1"
                     /translation="MDFDFSDDQEMLRDTVRKWATKAYTFERRRAIAKAGGFSPEAWK
                     ELAELGLLGLYVSERNGGMGFGPIDAMVVMEELGRAIVMEPFAAVALVATSLLNVGDA
                     PSAALWLQGIAEGKERVVLAHQERAARYRLAHVETSAANVGGTWQLTGTKTVVPAGAH
                     AGAFIVPARVSGAVDDPAGIALFLVSKGEKGVAVRGYPTQDGACAADLLLNDADAIEL
                     LPPGQAFAALEHAIDIGIAALCAEAVGAMDQLVAVTAEYMNTRKQFGVAIGTFQALRH
                     RIADVKMQLELARSMSYFASLKLNEPAPARRRALAQAKLQLGQSMRFVGQQCIQLHGG
                     IGVTDEYIASHYFKRLTVMEMSYGDTLHQLGEVSARMKDTAGVFA"
     gene            complement(2270..>3003)
                     /locus_tag="VNU48_04115"
     CDS             complement(2270..>3003)
                     /locus_tag="VNU48_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI10493.1"
                     /translation="QYIVNGQKTWTTLGQYGDWIFCLVRTSNEGKQTGISFLLIDMKS
                     PGVSVRPIITMDGEHEVNEVWFDNVEVPADQLIGEENKGWTYAKHLLAHERTNIADVN
                     RAKRELERLKRVAKAEGVYEDTRFRDAIALLEVEVVALEMMVLRVLSAEKSGKAALDV
                     AGLLKIRGSEIQQRYSELMMLAAGPASVPLIREAMEAGWQGDQASGAAFVAPLAGTYF
                     NLRKTTIYGGSNEVQRNIVAQTVLG"
BASE COUNT          386 a         1084 c         1055 g          478 t
ORIGIN      
        1 gatgtcggcg gccgccggca gccccggcag cgcgttcgcg gcgcgtaccg cgcgcacgat
       61 cgcggtcgcc gtcacctcgg cggcgagcgc gccgagcagc gtgacgttgg ccgtgcgccc
      121 ggccgcgccg gtggcgagcg cgaagatcgt gtcgccgtcg gtcatcgtgt gcaccgggtt
      181 gatcgcgcgc gcgaagccgt cgtgcgccat ctgcgccatc ttgttggcct gcgccttggt
      241 cagcaccgcg tcggtcgcga cgatgccgag cgtggtcgcg gcgccgacct gcaacggcgc
      301 gggaagctcg ccgcgcagca gcgcctgcat cgtgccgagc agcgcgctgc catcgggtgt
      361 gcgcgcaccg gcgatcacgc gcccggtggc cggatcgacg acgtcgccga tcgcattgac
      421 cgcgaccagc gccgcgaccg tgatgccgtc gaccacgacc gacgcgctgc ccagcccgcc
      481 gcgcatcgcg cgggccatgc cgaacagctt gccgaccgag cagcccgcgc cggcgccgac
      541 gttgccctgc gccaccgccg cgtcgctcgc cgcctcgcag gcggcgtagc cggccgcggc
      601 gtcggggcgg atcgacgggt cgccgaccca caggtcgaac aggatcgcgg cgggaacgat
      661 gggcacccga atcggcctgc cgattcgcgc gtggccctgt gcggggccga ccggcacgcc
      721 gaagccgcgc tcgtcgagcc agcgcatcac gccgccggcg gcgtccaggc cgaacgcgct
      781 gccgccggcg agcagcagcg cgtgcacggc ctcgaccgcg ttcagcggcg acagcagctc
      841 ggtctcgcgc gtgccgggtg cggcgccgcg cacgtcgacg ccggcgaccg caccttcggg
      901 cgtcaggatc acggtgcagc cggtcggccg ccgcgcgtcg gtgaagtggc cgacgcgcag
      961 cccggcgacg tcggtgagcg tgccgttgct tgccacgtcg aggcggcccg gctcagatca
     1021 cgcgaacacg ccggcggtgt ccttcatgcg cgccgacacc tcgccgagct ggtgcagcgt
     1081 gtcgccgtag ctcatctcca tcaccgtcag gcgcttgaag tagtggcttg cgatgtattc
     1141 gtcggtcacg ccgatgccgc cgtgcagctg gatgcactgc tggccgacga agcgcatcga
     1201 ttgcccgagc tgcagcttgg cctgcgcgag cgcgcggcgc cgcgcgggcg ccggctcgtt
     1261 cagcttgagg ctggcgaagt agctcatcga gcgcgcgagt tcgagctgca tcttgacgtc
     1321 ggcgatgcga tgccgcagcg cctggaacgt gccgatcgcc acgccgaact gcttgcgcgt
     1381 gttcatgtac tcggccgtga cggcgaccag ctggtccatc gcgccgacgg cctcggcgca
     1441 cagcgcggcg atgccgatgt cgatcgcgtg ctcgagcgcc gcgaaggcct ggcccggcgg
     1501 cagcagttcg atcgcgtcgg cgtcgttcag cagcaggtcg gccgcgcagg cgccgtcctg
     1561 cgtcgggtag ccgcgcaccg cgacgccctt ctcgcccttc gagacgagga acagcgcgat
     1621 gcccgccggg tcgtcgaccg cgccgctgac gcgtgccggc acgatgaacg cgcccgcatg
     1681 cgcgccggcc ggcacgacgg tcttcgtgcc cgtcagctgc caggtgccgc cgacgttcgc
     1741 agcgctggtc tcgacatgcg cgaggcggta gcgtgccgcg cgctcctggt gcgcgagcac
     1801 gacccgttcc ttgccctcgg cgatgccctg cagccacagc gccgccgacg gcgcatcgcc
     1861 gacgttgagc aggctggtgg cgaccagcgc gaccgcggcg aacggttcca tcacgatggc
     1921 gcggccgagc tcttccatca cgaccatcgc atcgatcggg ccgaagccca tgccgccgtt
     1981 gcgctcggac acgtacagcc cgagcaggcc gagttcggcc agttccttcc aggcctcggg
     2041 cgagaagccg ccggccttgg cgatcgcgcg gcggcgctcg aaggtgtagg ccttggtcgc
     2101 ccacttgcgc accgtgtcgc gcagcatttc ctggtcgtcg ctgaaatcga agtccatctc
     2161 tcggtcctct gtcttgtccg tctgactgga gccccttcct cctcgggggg aaggggctgg
     2221 ggaagggagc agccctgttg cgtctgcgct cgcgcagacg gccctcggct cagccgagga
     2281 ccgtctgggc gacgatgttt cgctgcactt cgttgctgcc gccgtagatc gtcgtcttgc
     2341 gcaggttgaa gtaggtgccg gccagcggcg cgacgaacgc ggcgccgctg gcctggtcgc
     2401 cctgccagcc ggcttccatc gcctcgcgga tcaagggcac gctggccggg ccggccgcca
     2461 gcatcatcag ctcgctgtag cgctgctgga tctcgctgcc gcggatcttc agcaggccgg
     2521 cgacatcgag cgcggccttg ccgctcttct cggcgctgag cacgcgcagc accatcatct
     2581 cgagcgcgac gacctcgact tccagcaacg cgatcgcgtc gcggaaacgc gtgtcctcgt
     2641 acacgccctc ggccttcgcg acgcgcttca gccgctcgag ctcgcgcttg gcgcggttca
     2701 cgtcggcgat gttggtgcgc tcgtgcgcga gcaggtgctt tgcgtaggtc cagcccttgt
     2761 tctcttcgcc gatgagctgg tcggccggca cctcgacgtt gtcgaaccag acctcgttga
     2821 cctcgtgctc gccgtccatc gtgatgatcg ggcgcaccga gacgccgggg ctcttcatgt
     2881 cgatcagcag gaagctgatg ccggtctgct tgccttcgtt gctggtgcgc acgaggcaga
     2941 agatccagtc gccgtactgg ccgagcgtgg tccaggtttt ctggccgttg acgatgtact
     3001 ggt
//
LOCUS       DATMJB010000185         2211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_788284, whole genome shotgun sequence.
ACCESSION   DATMJB010000185 DATMJB010000000
VERSION     DATMJB010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788284"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VNU48_04120"
     CDS             complement(<1..288)
                     /locus_tag="VNU48_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HWI10494.1"
                     /translation="MFPQAQAVLALADGTVFRGISIGAAGHTVGEVVFNTALTGYQEI
                     LTDPSYCRQIVTLTYPHIGNYGVNAEDVEATKVHAAGLIIKDLALRESNFRS"
     gene            complement(563..970)
                     /locus_tag="VNU48_04125"
     CDS             complement(563..970)
                     /locus_tag="VNU48_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWI10495.1"
                     /translation="MNHKPFLTLDEVKRIAAAAEAEATTNGWRVTIAIVDDGGHLLWL
                     QRLDGAAPVSAHIAPAKARTAALGRRESKTYEDMINQGRTSFLSVPEIEGLLEGGVPV
                     MVDGHCAGAVGVSGVKSDEDAQIAKAGIAALGA"
     gene            1148..1849
                     /locus_tag="VNU48_04130"
     CDS             1148..1849
                     /locus_tag="VNU48_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HWI10496.1"
                     /translation="MDQPLQATGYAGYTGEVTSQEQRNRVLRNTYWLLALSMVPTVLG
                     AWIGVATGITRAMGPGMSAIVFLVGAFGLIFAIEKTKNSAAGVPILLGFTFFMGLMLS
                     RLIGLVLGMNNGAGLIMTAFAGTGVIFLGMATLSTVIKRDLSSMGKFLFIGLIMLIVA
                     GIANIFLQSSALMITLSVLAIGIFSAFIIYDLKRVRDGEETNYISATLAIYLDLYNIF
                     QSLLSILGIFGGSRD"
     gene            complement(1948..>2211)
                     /locus_tag="VNU48_04135"
     CDS             complement(1948..>2211)
                     /locus_tag="VNU48_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase"
                     /protein_id="HWI10497.1"
                     /translation="DKWLVDPPREGAFALAKALAEVHADASIAPGYQAPARIVYVSCN
                     PATLARDAGLLVHQAGYRCSAAGAVNMFPHTAHVESIAVFDKA"
BASE COUNT          335 a          733 c          736 g          407 t
ORIGIN      
        1 gctgcgaaag ttcgattcgc gcagcgccag gtccttgatg atcaggccgg ccgcgtgcac
       61 cttcgtcgcc tcgacgtctt cggcgttgac gccgtagttg ccgatgtgcg ggtaggtcag
      121 cgtgacgatc tggcggcagt agctcgggtc ggtgaggatt tcctggtagc cggtcagcgc
      181 ggtgttgaac accacctcgc cgaccgtgtg gccggcggcg ccgatcgaga tgccgcggaa
      241 caccgtgccg tctgcgagtg ccaggaccgc ctgggcttgt ggaaacagca gttgcgcggg
      301 caccggattc tccgttctgt gcgcgcccag ccctgccgtt cgagcggatg ctcgtcgagc
      361 ctggaacagt cgagaaaacc gaaggagttt tcgtcgcgag caccggcgcg ttccagctct
      421 ggacggcgcg gttttcgctg ggcggatggg ttaagggtaa acctgcaaat tatagaccat
      481 gccactcgcg cccgactcgg cacgaaggcc ccctcatagc gcggccggcg cggcatcggc
      541 tgccgcggcg ccgctgattt cttcaggcgc cgagcgcggc gatgccagcc ttcgcgatct
      601 gcgcatcctc gtccgacttg acgccgctga cgccgaccgc gcccgcgcag tgaccatcga
      661 ccatcaccgg cacgccgccc tcgagcaggc cctcgatctc gggcacgctg aggaacgagg
      721 tgcggccctg gttgatcatg tcctcgtagg tcttgctctc gcgccggccc agcgccgcgg
      781 tgcgtgcctt cgccggcgcg atgtgggccg ataccggcgc ggcgccgtcc agccgctgca
      841 gccacagcag gtggccgccg tcatcgacga tcgcgatcgt gacgcgccag ccgttggtgg
      901 tggcttcggc ctcggcggcc gcggcgatgc gcttgacctc gtccagcgtc aggaagggct
      961 tgtggttcat ggtgtgctcg tggcgttgga atcagccgcg aactgtatgc aagaacctgg
     1021 ccgagttcga cgcgggtcgt gcgcggaacc atttcgccac ggcccacaag gacatctggc
     1081 ggggcgcgat cagggaacta gaatccgcgc aacgggtcac agcccgggta ttcatcaagg
     1141 aggctccatg gatcaacccc tgcaagcgac cggctacgcc ggctacaccg gcgaggtcac
     1201 ctcacaggag cagcgcaacc gcgtgctgcg caacacttac tggctgctcg cgctgtcgat
     1261 ggtgccgacg gtgctcggcg cctggatcgg cgtggccacc ggcatcacgc gggccatggg
     1321 ccccggcatg agcgcgatcg ttttcctggt cggcgcgttc ggcctgatct tcgcgatcga
     1381 aaagaccaag aactcggcgg cgggcgtgcc gatcctgctc ggcttcacgt tcttcatggg
     1441 cctgatgctg tcgcgcctga tcggcctggt gctgggcatg aacaacggcg ccggcctgat
     1501 catgacggcg ttcgccggca ccggcgtgat cttcctcggc atggccacgc tgtcgacggt
     1561 gatcaagcgc gacctgtcgt cgatgggcaa gttcctgttc atcggcctga tcatgctgat
     1621 cgtcgcgggc atcgcgaaca tcttcctgca gtcgagcgcg ctgatgatca cgctgtcggt
     1681 gctggcgatc ggcatcttct cggccttcat catctacgac ctgaagcgcg tgcgcgacgg
     1741 cgaggagacg aactacatca gcgccacgct ggcgatctac ctggacctgt acaacatctt
     1801 ccagtcgctg ctgtcgatcc tcgggatctt cggtggcagc cgcgactagt tcgacgcttc
     1861 gaaaatcgaa cgcttcgctg gatctacaag gccccgtcgg ggccttgttc tttttggctt
     1921 cgccgaagcg acagggtcgc gtcgccttca ggccttgtcg aagaccgcga tgctctcgac
     1981 gtgcgcggtg tgcgggaaca tgttcaccgc gccggccgcg ctgcagcggt agccggcctg
     2041 gtgcaccagc agcccggcat cgcgcgccag cgtcgccggg ttgcagctga cgtagacgat
     2101 gcgcgccggc gcctggtacc cgggcgcgat cgacgcatcg gcatgcacct cggccagcgc
     2161 cttcgcgagc gcgaacgccc cttcgcgcgg cgggtcgacc agccacttgt c
//
LOCUS       DATMJB010000186         9203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_100702, whole genome shotgun sequence.
ACCESSION   DATMJB010000186 DATMJB010000000
VERSION     DATMJB010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100702"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..253)
                     /gene="ffs"
                     /locus_tag="VNU48_04140"
     ncRNA           complement(155..253)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VNU48_04140"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(303..431)
                     /locus_tag="VNU48_04145"
     CDS             complement(303..431)
                     /locus_tag="VNU48_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10498.1"
                     /translation="MHAVERGAAVRQSGQIVARGQRTDALERVGEFGALLLETTVL"
     gene            426..896
                     /locus_tag="VNU48_04150"
     CDS             426..896
                     /locus_tag="VNU48_04150"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWI10499.1"
                     /translation="MHDELAVRGRPEPLMSLALMDIDRFKLINDGHGHAVGDIVLQRF
                     GQCAKTAVRQGDMLARWGGEEFLFVMPATASTQAIAAMERVREALRHVSFDDVAPGLV
                     VTFSAGVAECAGERDLEAAIARADAAMYEAKRAGRDRVTAAAPRPHCESAKVAA"
     gene            complement(1143..3575)
                     /gene="lnt"
                     /locus_tag="VNU48_04155"
     CDS             complement(1143..3575)
                     /gene="lnt"
                     /locus_tag="VNU48_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWI10500.1"
                     /translation="MLAGILASAVLSCLYARGGSGWLLGFVALVPWLRTLDANRTLAA
                     ALLGACAMSVAYTAAVFAWFGAAIGDYTRLGAATGLAVLLLAAPLFQPQFFAFAVVRH
                     LAGRRHGPVLRALAGAAAWVATEWLVPRLLGDTFGYGLYPSRLLRQAADLGGAAGLTV
                     LLLLANEAIAAALARRGERVRAIAKPLALAALVPLLLAGYGLAKLAAAPAAAGKPLRV
                     ALVQSNIVDYERLRRERGAAAVVREVLDTHYAMSYDAVEHRHADAVLWSETVYPTTFG
                     HPKSEAGAELDREILGIVDAARVPFVFGTYDRDAAGEYNAAAFVVPGTGLLGLYRKTR
                     VFPLTEYVPAWLDGPLLRRWLPWAGSWQPGSGARVFPLRLADGREIPALPLICLDDVD
                     AGQAIDGARLGAQAILTMSNDSWFTEQPQGAELHLAAAAFRSIETRLPQFRVTTNGYS
                     AVIDATGSVIAASRIGERTLVIGDVPVPEPPRTLMVIWGDWVGPAAAGFLVLLAATVA
                     LRAWRGRGVDEASASTGAAAVPADVAVLPPAARLAAGLLRAFARASLLAMAAAAALGE
                     GALQANTLVQIRTFAALFLVPEAAAWCVLRAFAARATIENGTLVLARGARRLELALRD
                     IIAVEPWRVPVPGPGASLRLASGARWRYGLAHADPMGLARALAAAGGAPVQQSTPTRA
                     TAYAQARLAIRRGRLDRPLAKFVLFPLALAIPAFRLHQHIAYGSAFGEYYSFGLAAYL
                     TTFALWWAAWAIGVTLCAAALRAAIEAGTLLAVLLRPAQAIAARRWLERLGLAALYLG
                     LPAWLLLRVLAN"
     gene            complement(3611..4342)
                     /locus_tag="VNU48_04160"
     CDS             complement(3611..4342)
                     /locus_tag="VNU48_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10501.1"
                     /translation="MDGPRRSGPRPTDPAGASRAWRPSLGYLAIALGIVAAGLVYGLM
                     VSATPEPVAAGTAAPPVLAGSASDSASAAPMRAMEASGSNPNAAPGAATAANGASAAA
                     AQRIARSRDPNGDLTPDISDYINNGEKPTMGEVIDRLHQAGVHTGLGAFSPPGTRPPL
                     VGLAVPEDFALPEGYVRHYQATDDGQRIEPILMYAPDHQFFDAAHQPIAIPENRVVPP
                     ELAPPGLPIRRVVIPAAVEPGRPGR"
     gene            complement(4361..5476)
                     /locus_tag="VNU48_04165"
     CDS             complement(4361..5476)
                     /locus_tag="VNU48_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWI10502.1"
                     /translation="MTTCIRAPRGSQLRSAASRMLLLALALLGCGAPAAANTLTQNVS
                     WTIDRAGTATKYRLVAYGDSIYAGYNGSITSAAKYAAPTVDAEYLSAQWNSDIESVRR
                     AKSGAVASDVYNNKIVAEKSYMQASTTRVVTFEMCGNDGLQARSSFKSQTGTCNYAGM
                     DAAVNSCRTYVAAAMDFINANAYAGTKAKIVSSLHYPGYAADNVQSSCRDAATGATVN
                     LQSIFLPRLASMNWAMCDFARQKGFQCADNFAQYMGADYDSNGDGQIDSVGLAYVLGE
                     TEASYVTRITSTLRSTLRDANTHFVSASTSYDYIQSDNTHPTYTGGTVNAGLFGGTTG
                     SGAPRYSSITGGKNPIWNQYGHERMGWAVSTSNPAAP"
     gene            complement(5653..6054)
                     /locus_tag="VNU48_04170"
     CDS             complement(5653..6054)
                     /locus_tag="VNU48_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10503.1"
                     /translation="MMGGIAAFLVAMAVGTSGYFTDDQLPPDGPVAATSAASALPVVQ
                     ESAETSPPAADAADATVVARLDPSADEAGACAGSCSDSEAGYAWAEAHRVGLQEDCAG
                     ASEPFNEGCRLYVEIQTALADHDDEKPSPGE"
     gene            6229..6378
                     /locus_tag="VNU48_04175"
     CDS             6229..6378
                     /locus_tag="VNU48_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HWI10504.1"
                     /translation="MTAALILIVLGVLFLLSNLGWLPRLGPLFAQWWPLILIIVGVSL
                     LIRRI"
     gene            6435..7607
                     /locus_tag="VNU48_04180"
     CDS             6435..7607
                     /locus_tag="VNU48_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWI10505.1"
                     /translation="MQAPVPATNTSDRVPLADVRDLLKVGEPLPFRVLDALERLLLNA
                     GQVLADEAQFAGLIERGAWAERHLVEAERATRAAGGGARAPQALSLFDRWERTLWQFD
                     KLSRALVRRQAKGAEVSALFATLCELVDVDADVALFLCVRQDDRRFALYPLTHALHCA
                     VLVLLTGRYLGWSQARVGALGCSALTMNLAILELQATMAEQDDPPTPRQLKDIRAHPQ
                     ASVKLLQDAGVVDEAWLAAIAEHHEQPGGGGYPHDLTEVCEAAQLLRAADVLMAKISP
                     RARRPAMAPQAAVRALFQQRPGDPLAMALIRTLGVYPPGSLLKLRSGEVAVAIRRPAA
                     GTHPLVATLSDTNGRPSAQTHRRDSAQPEFNVLGALVDAPGFARVLPERVYGIVGA"
     gene            7678..7872
                     /locus_tag="VNU48_04185"
     CDS             7678..7872
                     /locus_tag="VNU48_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10506.1"
                     /translation="MDLEQLMGRYFRLKQELAIAYKAQPWQSGRIDRLADDLAATERD
                     IAAMQPADEQYSDPTMSFAR"
     gene            7981..9168
                     /locus_tag="VNU48_04190"
     CDS             7981..9168
                     /locus_tag="VNU48_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI10507.1"
                     /translation="MPALALAAFGSGLSLRLTDALLPLFARQFGIGLGQAASVVTAFS
                     VAYGLSQLFFGPLGDRFGKYRVIAWACAACALTASLCGLAQGFTQLVLARLLAGATAA
                     AIIPLSMAWIGDVVPYEDRQPVLARFLIGQILGLSAGVFTGGFAADHLSWRVPFFAIG
                     AGFAAISAMLFAFDRRLPAQARALRRADGHVLARMVDEFSQVLATPWARVVLVTVFLE
                     GGFVYGSFAFIATHLHRTFGIALASAGSTVMLFGFGGLLFALASAALVRRLGEVGLTG
                     VGGVVLASALLAVGLAPAWWWAMPGCFVAGLGFYMLHNTLQINATQMAPQRRGAAVSA
                     FASCFFLGQAAGVAAAGVLVAKIGTGSVIAFGALGALAVALCFSRLRSRRRAADGGLD
                     AAA"
BASE COUNT         1317 a         3218 c         3284 g         1384 t
ORIGIN      
        1 catcaaaagt ctgggggcgc catttgcgtg cgattacctg gtaggtcata aggacgaagg
       61 atgagggatg aaggatgagg gatgaagtaa aagcgttacc gactggtcaa agctttcatc
      121 cttcatcctt ccgccttcac cccttgattt ggccagactc cggacttacc cgcaacacac
      181 gcgcgcctgg ctaccgttgc tccgttcccg gcctggcggg gttcaccggc gctacgttgt
      241 gcggagcccg aagtctgata acttgtcaaa gcacctgcga ttcgccatgc gaatcggcgc
      301 cgctagagta cagtggtctc gagcagcagc gcgccgaact cgccgacgcg ctcgagcgca
      361 tccgtgcgct ggccacgcgc gacgatctga ccggactggc gaaccgccgc gccgcgctcg
      421 accgcatgca cgacgagctc gccgtgcgcg ggcgccccga gcccctgatg agcctggcgc
      481 tgatggacat cgatcggttc aagctgatca acgatggcca cggccacgcc gtcggcgaca
      541 tcgtgttgca acgcttcggc cagtgcgcca agacggcggt gcggcagggc gacatgctgg
      601 cgcgctgggg cggcgaggag ttcctgttcg tgatgccggc caccgcgtcg acgcaggcca
      661 tcgcggcgat ggaacgggtg cgcgaggcgc tgcgccacgt gtctttcgac gacgtcgccc
      721 ccggcctggt ggtcacgttt tcggccggcg ttgccgagtg cgccggagag cgcgacctgg
      781 aggccgccat cgcccgcgcc gatgcggcca tgtacgaagc gaagcgcgcc ggccgcgacc
      841 gcgtcacggc ggccgctccg cgcccgcatt gcgagtcagc caaggtcgct gcctgagcgc
      901 agcgatggag acggcgtcgt cacgtgtcac tggcgatctg attggtgagg gtgccaattc
      961 gcctcaccgc cgtctgatga aggtatcgga tttcgccgcg ttccacttga gcgcgctcgt
     1021 tcacgagact cgagaactaa gggcgcttcc cggcggtcgg gtgacgcaag gcgtgtgccg
     1081 tcagggacgc ggcaccacct gaacgcccga gaccgggcca agcaccggcg ctgctgccga
     1141 cctcaattcg cgagcacccg gagcagcagc cacgccggca agccgaggta gagcgccgcg
     1201 aggccgaggc gctcgagcca gcggcgcgcg gcgatggcct gtgcggggcg cagcagcacc
     1261 gccagcagcg tgccggcttc gatcgcggcg cgcagcgccg cggcgcacag cgtcacgccg
     1321 atcgcccagg cggcccacca gagcgcgaag gtcgtcaggt acgccgccag gccgaagctg
     1381 tagtactcgc caaaggcgct gccgtaggcg atgtgctggt gcagcctgaa cgccggaatc
     1441 gcgagcgcga gcgggaacag gacgaacttc gccagtgggc ggtcgagccg cccgcgccgg
     1501 atggccaggc gcgcctgcgc atacgcggtc gcgcgcgtgg gtgtgctctg ttgaaccggt
     1561 gcgccaccgg ccgcggccag cgcgcgggcc aggcccatgg gatcggcgtg cgcgagcccg
     1621 tagcgccagc gcgcgcccga ggccagccgc aacgaggcgc cgggaccggg gaccggcacg
     1681 cgccacggct cgacggcgat gatgtcgcgc agcgcgagtt cgaggcgccg tgcgccgcgt
     1741 gccagcacga gcgttccgtt ctcgatggtg gcgcgcgcag cgaacgcgcg cagcacacac
     1801 cacgcggcgg cctcggggac gagaaagagc gcggcgaacg tgcggatctg cacgagcgtg
     1861 ttcgcctgca aggcgccctc acccagcgcg gccgcggcgg ccatcgcgag caggctggcg
     1921 cgcgcgaagg cccgcagcag gccggcggcg aggcgcgccg cgggcggcag cacggcgaca
     1981 tcggcgggga cggccgccgc accagtcgat gccgacgctt cgtcgacgcc tcggccgcgc
     2041 catgcgcgca aggccacggt cgcggccagc agcacgagga agcccgccgc cgcgggcccg
     2101 acccaatcgc cccagatcac catcaacgtg cgcggcggct caggtaccgg cacgtcgccg
     2161 atcaccagtg tgcgttcgcc gatgcgcgac gcggcgatca cgctgccggt cgcgtcgatc
     2221 accgcgctgt agccgttcgt cgtgacgcgg aactggggca gacgcgtttc gatgctgcga
     2281 aacgccgccg ccgccaggtg cagttcagca ccctgtggtt gctcggtgaa ccaagagtcg
     2341 ttcgacatcg tcaggatcgc ctgcgcgccc agccgcgcgc cgtcgatggc ctggcccgca
     2401 tccacgtcgt cgagacagat cagcggcagc gccgggatct cacggccgtc ggccaggcgc
     2461 agcgggaaca cgcgcgcgcc gctgcccggt tgccagctgc cggcccacgg cagccaacgg
     2521 cgcagcagcg gcccgtcgag ccacgccggc acgtactcgg tgagcgggaa gacgcgcgtc
     2581 ttgcgataca aaccgagcag tcccgtgccg ggcaccacga acgcggccgc gttgtattcg
     2641 ccggcggcgt cgcgatcgta ggtgccgaac acgaacggca cccgggcggc gtcgacgatg
     2701 ccgaggatct cgcggtcgag ctcggcgccg gcctcgctct tcggatgccc gaaggtcgtc
     2761 gggtacaccg tctccgacca cagcaccgcg tcggcgtgcc ggtgctcgac ggcgtcgtag
     2821 ctcatcgcgt aatgcgtgtc gagcacctcg cgaacgaccg cggctgcgcc ccgctcgcgg
     2881 cgcaggcgct catagtccac gatgttcgat tggacaagcg cgacgcgcaa tggcttgccg
     2941 gccgccgcgg gcgcggccgc gagcttcgcg aggccgtagc ccgccagcag cagcggcacc
     3001 aacgcggcaa gcgccaatgg cttcgcgatc gcgcgcacgc gctcgccccg gcgcgcgagc
     3061 gccgccgcga tggcttcatt cgccagcagc agcaggaccg tgaggcccgc ggccccgccg
     3121 agatcggcgg cctggcgcag caggcgcgag gggtagagcc cgtagccgaa cgtgtcgccg
     3181 aggagcctgg gcacgagcca ctcggtcgcc acccaggccg cggcgcccgc gagcgcgcgc
     3241 aggaccgggc cgtgccggcg gccggcgaga tgacgcacga ccgcgaaggc gaagaactgc
     3301 ggctggaaca gcggcgcggc aaggagcagc acggccagcc cggtggcggc gccgagccgc
     3361 gtgtagtcgc cgatcgccgc gccgaaccag gcgaagaccg ccgccgtgta cgccaccgac
     3421 atcgcgcagg cgccgagcag cgcggccgcg agcgttcggt tcgcatcgag cgtgcgcaac
     3481 cacggcacca gggccacgaa accgagcagc cagccgctgc cgccgcgcgc gtacaggcac
     3541 gacagcaccg cgctggcgag gatgccagcc agcatgcggg cgctggaccg gggcgaaggc
     3601 gcggcggtgt tcagcgcccg ggtctccccg gttcgaccgc cgccgggatc acgacgcgtc
     3661 ggatcggcaa ccccggcggc gcgagttccg gcggtacgac gcggttctcc gggatcgcga
     3721 tcggctggtg ggccgcgtcg aagaactggt ggtccggcgc gtacatcagg atcggctcga
     3781 tgcgctggcc gtcgtcggtc gcctggtagt ggcggacgta gccctcggga agcgcgaagt
     3841 cctcgggcac cgcgagcccg accagcggcg gccgggtgcc gggcggcgag aaggcgccga
     3901 ggccggtatg cacgccggcc tggtgcaggc ggtcgatcac ctcgcccatc gtcggtttct
     3961 cgccgttgtt gatgtagtcc gagatgtcag gcgtcagatc gccgttcgga tcgcgcgagc
     4021 gtgcaatgcg ttgcgccgcg gcggcagacg cgccgttcgc cgcggtcgct gcgcctggcg
     4081 ccgcgttggg gttggagccg ctcgcttcca ttgcgcgcat gggggccgcg ctggccgagt
     4141 cgctcgccga accggccagc accggagggg ccgccgtgcc ggcggccaca ggctccggcg
     4201 tcgcggacac catcaacccg tagacgaggc ctgccgcgac gatccccagc gcgatcgcga
     4261 gatagcccag cgacgggcgc cacgcgcgac tggcgccggc cggatcggtc ggcctcgggc
     4321 cagaacgacg cggcccgtcc atcgggacgc ccgccgagcc tcagggcgcc gccgggttgg
     4381 aggtcgacac cgcccagccc atgcgctcgt ggccgtactg gttccagatc gggttcttgc
     4441 cgccggtgat cgagctgtag cgcggcgcgc ccgagccggt ggtgccgccg aacaggccgg
     4501 cgttcacggt gccgccggtg taagtcgggt gcgtgttgtc ggactggatg tagtcgtagc
     4561 tggtgcttgc ggacacgaaa tgcgtgttcg cgtcgcgcag cgtcgaacgc agcgtcgacg
     4621 tgatgcgggt gacgtagctc gcctcggtct cgcccagcac gtaggcaagc ccaaccgagt
     4681 cgatctgccc atcgccgttg ctgtcgtagt ctgcacccat gtactgggcg aagttgtccg
     4741 cgcactggaa gcccttctgc cgggcgaagt cgcacatcgc ccagttcatc gaggcgagcc
     4801 ggggcaggaa gatgctctgc aggttcaccg tcgcgccggt cgcggcgtcc ctgcacgagc
     4861 tctgcacgtt gtcggcggca taacccgggt agtgcaggct cgagacgatc ttcgccttgg
     4921 tgcccgcata ggcattggcg ttgatgaagt ccatcgcggc cgcgacgtag gtccggcagc
     4981 tgttgaccgc cgcgtccatc ccggcatagt tgcaggtgcc ggtctggctc ttgaagctcg
     5041 aacgcgcctg cagcccgtcg ttgccgcaca tctcgaaggt gaccacgcgg gtcgtgctgg
     5101 cctgcatgta cgacttctcg gccacgatct tgttgttgta gacatccgag gcgaccgcgc
     5161 cggacttcgc gcggcgcacg ctctcgatgt cgctgttcca ctgcgccgag aggtactccg
     5221 cgtccacggt gggcgccgcg tacttcgcgg cgctggtgat ggagccgttg tagcccgcgt
     5281 agatcgaatc cccgtaggcc acgaggcggt acttggtcgc ggtacccgcc cggtctatgg
     5341 tccacgagac attctgggtc agcgtgttgg ccgctgccgg cgcaccgcag cccagcaggg
     5401 ccagcgcgag caacagcatg cgcgaggcgg cgctgcgcag ctgtgatcca cgcggcgcgc
     5461 gaatacaagt cgtcatctgc gtctccccgg tctcgtgatc tgctttggga accgagctcc
     5521 cgaatccgtg cggattgatg ggtgcacggc cagtgtggac caaaaccggg tgcgcgccaa
     5581 agggggcgcc gtccggagta tccctaaccg gtcgcagaac cggggacgat atcgggcggc
     5641 accggcccga actcactcgc ccggcgaagg cttctcgtcg tcgtgatcgg ccagtgcggt
     5701 ttgaatctcg acgtacagcc tgcagccctc gttgaacggc tcggaggcgc cggcgcaatc
     5761 ctcctgcagg ccgaccctgt gcgcctcggc ccacgcgtaa ccggcttcac tgtccgaaca
     5821 gctgccggcg caagccccgg cctcgtctgc cgacgggtcg agcctggcaa cgacagtggc
     5881 atccgcggca tccgcggccg gcggggaagt ctcggccgac tcctggacga cgggcagcgc
     5941 cgacgccgcc gaggtcgcag caaccgggcc gtccggcggg agctggtcgt cggtgaagta
     6001 gcccgaggtg ccgacggcca tcgccacgag gaatgccgcg atgccgccca tcatgatcca
     6061 aggagatcgc atagagcctc cgacgattga gtttgtgcag gcgagaagat cttgcggatt
     6121 atgcggaggt cgtcgacggg ttcatcgcgg gcggcaggtc gagccacgcc gtgtggcatg
     6181 ctttggcatc cgggatgcaa caggaaactt cgcatgcgcc acagatcgat gaccgccgcc
     6241 ctgatcctga tcgtgctggg tgttctgttt ctgctgtcca atctcggctg gctgccgcgc
     6301 cttggcccgc tgttcgccca atggtggccg ctcatcctga tcatcgtcgg cgtttccctg
     6361 ctcattcggc gcatctagtg ttccccgaag gcaagcactg acggaccaag atgggcctcc
     6421 ccgggggaac cgcaatgcaa gcacctgtcc ctgcgaccaa cacgtcggat cgggttcccc
     6481 tggccgacgt tcgagacctg ctcaaggtcg gcgagccctt gccgttccgc gtcctggacg
     6541 cactcgagcg actgttgctg aacgcgggcc aggtgctcgc cgacgaggcc cagttcgccg
     6601 gcctgatcga gcgcggtgcg tgggccgagc gtcaccttgt cgaagccgag cgggcgacgc
     6661 gcgccgcggg cggtggcgca cgcgcgcccc aggcgctgtc gctgttcgac cgctgggagc
     6721 gcacgctctg gcagttcgac aagctcagcc gcgcgctggt gcgccgccag gccaagggcg
     6781 ccgaggtgtc cgcgctgttc gcgacgctgt gcgagctggt cgacgtcgat gccgacgtgg
     6841 ccctgttcct gtgcgtgcgg caggacgacc gccgcttcgc cctgtacccg ttgacgcacg
     6901 cgctgcattg cgccgtgctg gtgctgttga ccggccgcta cctcggctgg tcgcaggcgc
     6961 gtgtcggcgc gctgggctgc agcgcgctga cgatgaacct ggcgatcctc gagctgcagg
     7021 ccacgatggc cgagcaggac gatccgccga ccccgcggca gttgaaggac attcgcgccc
     7081 acccgcaggc gtcggtgaag ctgctgcagg acgcaggtgt cgtcgatgaa gcctggctgg
     7141 cggcgattgc cgagcaccac gagcagcccg gcggtggcgg gtatccgcac gacctgaccg
     7201 aggtctgcga ggccgctcag ctgctgcgcg cggccgatgt gctgatggcc aagatcagcc
     7261 cgcgcgcgcg gcgcccggcg atggcgccgc aggccgcggt gcgcgcgctg ttccagcaac
     7321 ggcccggtga cccgcttgcg atggcgctga tccgcacgct cggcgtctac ccgcccggca
     7381 gcctgctgaa gctgcgctcg ggtgaagtcg ccgtcgcgat ccgccggcct gccgccggca
     7441 cgcacccgct ggtcgccaca ttgagcgaca cgaacggtcg gcccagcgcg cagacgcatc
     7501 gccgcgactc ggcgcaaccg gagttcaacg ttctaggcgc actcgtcgat gcgccgggtt
     7561 tcgcgcgcgt gctgccggag cgcgtctacg gcatcgtcgg cgcttgacga cggggcattg
     7621 tccgacaccg gccgccgtgc ggattgttca tactgcaccg cagcaaagga gcgctgtatg
     7681 gacctcgaac aattgatggg gcggtacttt cggttgaagc aggaactggc gatcgcctac
     7741 aaggcgcagc catggcagag cggccgcatc gaccgccttg ccgacgatct cgccgcgacc
     7801 gagcgcgaca tcgccgcaat gcaacctgcc gacgagcagt acagcgatcc gacgatgagc
     7861 ttcgcgcgct gacgcggctc acatcgctca cgcgctgcgc cttgcgcgcc gagccgtgac
     7921 aatggccgga tgccttcggc cgacgcgccc gcttccacaa gcctcatctc ccgtttcgcc
     7981 gtgccggccc tggcactggc ggccttcggc agcggcctgt cgctgcgcct gaccgacgcg
     8041 ctgctgccgc tgttcgcacg ccagttcggc atcggcctgg gccaagcggc gagcgtcgtg
     8101 acggcgttct cggtcgccta cgggttgtcg cagctgttct tcggcccgct cggtgaccgc
     8161 ttcggcaagt accgcgtgat cgcctgggcc tgcgcggcct gcgcgctgac cgcgtcgctg
     8221 tgcggcctgg cgcagggctt cacgcaactc gtgctcgcgc ggctgctcgc cggcgccacc
     8281 gccgcggcga tcatcccgtt gtcgatggcc tggatcggcg acgtcgtgcc ctacgaagac
     8341 cgccagccgg tcctcgcacg cttcctgatc gggcagatcc tgggtctgtc ggccggcgtg
     8401 ttcaccggcg gcttcgcggc cgaccacctg agctggcgcg tgccgttctt cgcgatcggc
     8461 gccggcttcg cggcgatcag cgcgatgctg ttcgcgttcg accgccgcct gcccgcccag
     8521 gcgcgcgcgc tgcggcgcgc cgacggccac gtgctggcgc gcatggtcga cgagttctcg
     8581 caggtgctgg ccacaccgtg ggcgcgcgtc gtgctggtca cggtgttcct cgaaggcggc
     8641 ttcgtctacg gctcgttcgc gttcatcgcg acccatctgc atcgcacctt cggcattgcg
     8701 ctggcgagcg ccggctcgac ggtgatgctg ttcggcttcg gcggtctgct gttcgcgctg
     8761 gcgtcggcag cgctggtgcg gcgcctcggc gaggtcggcc tgaccggcgt cggcggtgtc
     8821 gtgctcgcca gcgcactgct cgcggtcggc ctggcgccgg catggtggtg ggcgatgccg
     8881 ggctgcttcg tcgcgggcct gggcttctac atgctgcaca acacgctgca gatcaacgcc
     8941 acgcagatgg cgccgcagcg gcgcggcgcc gcggtgtcgg cgttcgcgtc gtgcttcttt
     9001 ctcggccagg ccgccggggt cgcggcggcc ggcgtgctgg tcgcgaagat cggcaccggc
     9061 agcgtgatcg cgttcggcgc gctcggcgcg ctcgcggtcg cgctgtgctt cagccgcttg
     9121 cgctcacggc ggcgcgcggc ggacggcggc ctcgacgccg cggcctgacc gcggcgggcc
     9181 ccgcgcgcgc cgagagcttc agc
//
LOCUS       DATMJB010000187         7411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_136600, whole genome shotgun sequence.
ACCESSION   DATMJB010000187 DATMJB010000000
VERSION     DATMJB010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136600"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VNU48_04195"
     CDS             <1..526
                     /locus_tag="VNU48_04195"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HWI10508.1"
                     /translation="APLVVVMGVSGCGKTTLGRLLAQRLNAEFLEGDDLHPPRNVERM
                     AAGIALTDADRRDWLLQIAQQLADAHASNHGLVVSCSALKRRYRDVLRAASAGLAFVH
                     LNADRALLEARMDARSDHFMPASLLDSQLQTLEPPTPAERAASFDAALPAAQIAEQAA
                     AWLAKLPPAPKARR"
     gene            564..947
                     /locus_tag="VNU48_04200"
     CDS             564..947
                     /locus_tag="VNU48_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10509.1"
                     /translation="MTTFTQVTLFTDTDGRARFREQTLALPEGTPSAMLSALMPSGGL
                     QLRHSPVGFRSSFHVTTTPQWVFILSGRMEINLQDGSARVFGPGEHFYSADTLPPGAS
                     FDPTLHGHWSRQLGDEPLVTLFVRA"
     gene            955..1719
                     /locus_tag="VNU48_04205"
     CDS             955..1719
                     /locus_tag="VNU48_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWI10510.1"
                     /translation="MIKNVHGGTVDLLGEAIVTGRYPVGSTLPPEPALCDELGVSRTV
                     IREAVKSLIAKGLLVTGPKLGTRVLAEEQWNWFDPDVVAWQSKAGLSREFLRDLQELR
                     RLVEPAAVRLAAERATAADIAEIEAAYAGMKLSIEEGGDYVSSDLRFHQGLLRAAHNR
                     MVAQMSKALGALLRTSFEISTSRPDGPAQSLPLHRAVLDAVIARSPQKAERAIIVLID
                     GANEDIEEVLASSRRKLPSLGVPAPRLKARAKAAMR"
     gene            1829..2581
                     /locus_tag="VNU48_04210"
     CDS             1829..2581
                     /locus_tag="VNU48_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI10511.1"
                     /translation="MNQIDLQGRVAVVTGGAQGIGYAAAERMLASGAAVVLWDIDAAR
                     LAQAENALATLGTVSSSIVELTLAADIDAAAAAAVKAHGRIDILVNNAGITGGNAPTW
                     ELSPDAWRRVIDVNLVGPFLTCRAVVPLMLKQGYGRIVNIASVAGKEGNPNASHYSAS
                     KAGLIALTKSLAKELATKGVLVNAVSPGAAKTAMFDQMTQAHIALMLGKIPMARFLEV
                     GEAAAMIAWLASEECSFSTGAVFDLSGGRATY"
     gene            2741..3238
                     /locus_tag="VNU48_04215"
     CDS             2741..3238
                     /locus_tag="VNU48_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HWI10512.1"
                     /translation="MNHQLPTFFRASLVAAALGSALVIAGCGSMSMGSSSSSPAAAAT
                     PMMATQTFRATLSSAAEVPANASAATGTLEASLDKASSVLRWKVTYSGLSGAATMAHF
                     HGPAMPGSNAGVVVPFPSAASPAEGSATLTPAQVADLMAGKWYVNVHTAQNPGGEIRG
                     QVLAN"
     gene            complement(3399..3830)
                     /locus_tag="VNU48_04220"
     CDS             complement(3399..3830)
                     /locus_tag="VNU48_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWI10513.1"
                     /translation="MNNLDDTDRELLALLRHDARLPVATLAAKLKVARGTVQNRMKRL
                     ERDGVIVGYTVRVKPQAEAHRIRALMTIVVEGNRGAEVLKALRGHPNVTGLHSTNGRW
                     DLIAELRADSLEAFDRALGNIRLIAGIASTETSLLLSTHKV"
     gene            3943..5028
                     /locus_tag="VNU48_04225"
     CDS             3943..5028
                     /locus_tag="VNU48_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HWI10514.1"
                     /translation="MTRFLDVPAMSRLVCEVGVARFLTELAQAIRADYLRWNEFDKSA
                     RLACHSPVGVIELMPVSDAQRFAFKYVNGHPRNTGRGLFTVMAFGVLAEVDTGYPLLL
                     SELTLTTALRTAATSALAARALANPGCRRMALIGNGAQSEFQALAFHTLLGIDEVRVF
                     DVDPAATDKLVRNLAATKGLRVIRAASTREAVRGAQIVTTVTADKTRATILTPEMIEP
                     GMHINAVGGDCPGKTELHADVLRRARVIVEYEPQTRIEGDIQQLPPDFPVTELWRVLS
                     GAAAGREHAGQVTVFDSVGFALEDYSALRYVHELALARGAGDDVALVPPGANPKDLFG
                     RLAEPADAAARDALASVLGALRRGQPA"
     gene            complement(5084..6178)
                     /locus_tag="VNU48_04230"
     CDS             complement(5084..6178)
                     /locus_tag="VNU48_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI10515.1"
                     /translation="MVDNGNGLQMHVLEAGDPSRPGVLLLHGFPELAYSWRKLLLPLA
                     QAGFHVFAPDQRGYGRSTGWDADYDGDLASFRLLNLATDALGLVAAMGHASLAAVVGH
                     DFGSPVAACCALARPDVFRAVVLMSAPFGGPPAFPVDTANAPPAAPVAPAADWRAELA
                     RLPRPRKHYQWYYSERGADAEMRHCSQGLHDFLRAYYHFKSADWSGNRPFALSARSAA
                     EFAKLPDYYVMDLHKGMSETVAPQMPTPAQVAACRWLPDAELAVYAAEFARTGFQGGL
                     QWYRCQTDGTSSAGLRLFAGRHIDVPSRFIAGRSDWGPWQWPGALQSMQDQACTRMRG
                     CDWVEGAGHWVQQEQPAEVARLLLQFLREV"
     gene            complement(6226..6462)
                     /locus_tag="VNU48_04235"
     CDS             complement(6226..6462)
                     /locus_tag="VNU48_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10516.1"
                     /translation="MKKRERLEEIAVPTLVVWGTPDFAPLGARMREIVQRIPQVASVV
                     MEGVAHLPGLEKPVEFNRAPLAFLESLPPEAAAP"
     gene            complement(6459..>7411)
                     /locus_tag="VNU48_04240"
     CDS             complement(6459..>7411)
                     /locus_tag="VNU48_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI10517.1"
                     /translation="ASAETVSLAVVPMFHITGMLYYVLGNIAIGCTSVILPRWDRELA
                     GRLISKYKVTHWTCIPTMIIDLFGSPNYKSFDLTSLTYLSGGGAAMPQAVAQRLLDEY
                     GLTFCEGYGLTETAAPTHSNPPERAKLQCLGIPVFGVDSRVVDPLTMKELPVGEVGEI
                     ISRGPMVFKGYWGQPEATREAFVEFEGQTFFRTGDLGRMDEEGYFFMTDRLKRMINAS
                     GYKIWPAEVEMLLYKHPAVQEACIIAARDAYRGETVKAVVVLRAEAKGKTTAEDITNW
                     ARENMAAYKVPKLVQFVDALPRSGSGKVMWRLLQDQEAAA"
BASE COUNT         1078 a         2670 c         2595 g         1068 t
ORIGIN      
        1 cgccccgctg gtggtcgtga tgggcgtcag cggctgcggc aagaccacgc tcgggcggct
       61 gctggcgcaa cgcctgaacg ccgagttcct cgagggcgac gacctgcacc cgccgcgcaa
      121 tgtcgagcgc atggccgcgg gcattgcgct gaccgatgcc gatcgccgcg actggctgct
      181 ccagatcgcg cagcagctcg ccgatgcgca tgccagcaac cacggcctgg tggtctcgtg
      241 ctcggcactg aagcgccgct accgcgacgt gctgcgcgcc gcgtccgccg ggctcgcgtt
      301 cgtgcacctg aatgccgatc gcgcgctgct cgaggcgcgc atggatgcgc gcagcgatca
      361 cttcatgccc gcgtcgctgc tggacagcca gttgcagacg ctggaaccgc caaccccggc
      421 cgagcgcgcc gccagcttcg acgcggcgct gcccgcggcg cagatcgctg agcaggcggc
      481 ggcgtggctg gcgaagctgc cgccggcacc gaaagccagg cgatgatcga cgccgccgct
      541 ctgaccacga cacagggttt tcgatgacca ccttcaccca agtcacgctg ttcaccgaca
      601 ccgacggccg cgcgcgtttt cgcgaacaga cactcgcgtt gcccgagggc acgccgagcg
      661 cgatgctgtc ggcgctgatg cccagcggcg gcctgcagct gcgccacagc ccggtcggct
      721 ttcgcagcag cttccacgtc acgaccacac cgcagtgggt gttcatcctg agcggccgca
      781 tggagatcaa cctgcaggac ggcagcgcgc gcgtcttcgg ccccggcgag cacttctact
      841 ccgccgacac gctgccgccc ggcgccagct tcgaccccac gctgcacggc cactggagcc
      901 gccagctcgg cgacgagccg ctggtcacct tgttcgtccg ggcctgacgc ggcgatgatc
      961 aagaacgtcc acggcggcac cgtcgacctg ctcggcgagg ccatcgtcac cggccgctac
     1021 ccggtgggca gcacgctgcc gcccgagccg gcgctgtgcg acgaactcgg cgtcagccgc
     1081 acggtgatcc gcgaggcggt caagtcgctg atcgcgaagg gcctgctcgt caccgggccg
     1141 aagctcggca cccgcgtgct ggccgaggag cagtggaact ggttcgaccc cgacgtcgtc
     1201 gcgtggcaat cgaaagccgg gctgtcgcgc gagttcctgc gcgacctgca ggagctgcgc
     1261 cgcctcgtcg agcccgccgc ggtgcgcctg gccgccgagc gcgcgaccgc cgccgacatc
     1321 gccgagatcg aggccgccta cgccggcatg aagctgtcga tcgaagaggg cggcgactac
     1381 gtcagcagcg acctgcgctt tcaccagggc ctgctgcgcg cagcccacaa ccgcatggtc
     1441 gcgcagatga gcaaggcgct gggcgcgttg ctgcgcacca gcttcgagat ctcgacctcg
     1501 cggcccgacg ggccggcgca gtcgctgcca ctgcaccgcg cggtgctcga tgcggtgatc
     1561 gcgcgctcgc cgcagaaggc cgagcgcgcg atcatcgtgc tgatcgacgg cgcgaacgaa
     1621 gacatcgaag aggtgctcgc gagctcgcgg cgcaagctgc cgtcgctggg cgtgccggcg
     1681 ccgcgcctga aggcgcgggc gaaggccgcc atgcgctgag cgccgcctgc ttcaccggat
     1741 cggcgcagag aatcgcttgc gccagacccc ggcggcgctg ctgcgacgcc gcgccgaacg
     1801 ccgaccagac gctgaagaga ctcgcacgat gaaccagatc gacctgcagg gccgcgtcgc
     1861 ggtcgtcacc ggcggcgcgc agggcatcgg ctacgccgcg gccgagcgca tgctcgcgtc
     1921 gggcgccgcc gtggtgctgt gggacatcga cgccgcgcgc ctcgcgcagg ccgagaatgc
     1981 gctcgccacc ctcggcaccg tcagcagttc gatcgtcgag ctgacgctgg cggccgacat
     2041 cgacgccgcc gcggccgccg cggtcaaggc gcacggccgc atcgacatcc tcgtcaacaa
     2101 cgccggcatc accggcggca atgcgccgac ctgggaactc tcgcccgacg cgtggcgtcg
     2161 cgtgatcgat gtcaacctcg tcggcccgtt cctgacctgc cgcgcggtcg tgccgctgat
     2221 gctcaagcag ggctacggcc gcatcgtcaa catcgcgtcg gtggcgggca aggaaggcaa
     2281 cccgaacgcc tcgcactaca gcgcgtccaa ggccgggctg attgcgctga ccaagtcgct
     2341 cgcgaaggag ctggcgacga agggcgtgct cgtcaacgcg gtctcgccgg gcgccgcgaa
     2401 gaccgcgatg ttcgaccaga tgacgcaagc gcacatcgcg ttgatgctcg gcaagatccc
     2461 gatggcgcgc ttcctcgagg tcggcgaggc cgcggcgatg atcgcctggc tcgcgtcgga
     2521 ggaatgctcg ttcagcaccg gcgcggtgtt cgacctgtcg ggcggtcgcg cgacctactg
     2581 aatacgccgg ccgacgcagc accgacatgc gccgtcgaat gacagcgcga cggcgcgtcg
     2641 tccgacaggc cggtgccgac gcccggttca tcctgcccgg tgcgccgcca cgcgaaacgc
     2701 gcgcagaatt gctgccccac ccgaaccctg gagactccgc atgaaccacc aactgcccac
     2761 cttcttccgc gccagcctgg tcgccgccgc gctcggcagc gcgctcgtga tcgccggctg
     2821 cggctcgatg tcgatgggtt cgagcagcag cagccccgcc gccgccgcga cgccgatgat
     2881 ggccacacag accttccgcg cgacgctgtc cagcgccgcc gaggtgccgg cgaacgccag
     2941 cgcggccacc ggcacgctcg aggcgagctt ggacaaggcc agcagcgtgc tgcgctggaa
     3001 ggtcacctac agcggcctga gcggtgcggc gacgatggcg cacttccacg gcccggcgat
     3061 gccgggctcg aatgccggcg tggtggtgcc gttcccgagc gcggccagcc ccgccgaagg
     3121 cagcgcgacg ctgaccccgg cgcaggtcgc cgacctgatg gccggcaagt ggtacgtcaa
     3181 cgtgcacacc gcgcagaacc cgggcggcga gatccgcggt caggtgttgg cgaactgatg
     3241 aagcacccgg cctcgcgggg tcgcgaggcc acctgcgcgg cgggtcacgg cttcagtccg
     3301 gcttccacct cccgccgtcg accttgacgc gcgtcggtgc gcgcgcgccg atcgcgcggc
     3361 gcggcgccgg tgatataccc cggccggcgc gcggcgcctc agaccttgtg cgtcgacagc
     3421 agcaggctcg tctcggtgct cgcgatgccg gcgatcagcc ggatgttgcc cagcgcccgg
     3481 tcgaaggcct cgagcgaatc ggcgcgcagc tcggcgatca ggtcccagcg gccgttggtg
     3541 ctgtgcagcc cggtcacgtt cgggtggccg cgcagcgcct tcagcacctc ggcgccgcgg
     3601 ttgccttcga ccacgatcgt catcagcgcg cggatgcggt gcgcctcggc ctgcggcttc
     3661 acgcgcaccg tgtagccgac gatcacgccg tcgcgctcga gccgcttcat gcggttctgc
     3721 accgtgccgc gcgcgacctt cagcttcgcg gccagcgtgg ccaccggcag gcgcgcgtcg
     3781 tggcgcagca gcgcgagcag ttcgcggtcg gtgtcgtcga gattgttcat attgctctcg
     3841 gcccctgaac aaattgactc aagagccgat catatcgaac agtctgctgt cttttcgttg
     3901 gctgcgaagc gccggaagat gctcccgata ccggagactt tcatgacccg ctttctcgat
     3961 gttcccgcca tgtcgcgcct ggtgtgcgag gtcggcgtgg cgcgcttcct gaccgagctg
     4021 gcgcaggcga tccgcgccga ctacctgcgc tggaacgagt tcgacaagtc cgcgcgcctg
     4081 gcctgccatt cgccggtcgg cgtgatcgag ctgatgccgg tttccgacgc gcagcgcttc
     4141 gcgttcaagt acgtcaacgg ccacccgcgc aacaccggcc gcggcctgtt cacggtgatg
     4201 gccttcggcg tgctcgccga ggtcgatacc ggctacccgc tgctgctgtc ggagctgacg
     4261 ctgacgaccg cgctgcgcac cgccgcgacc tcggcgctgg ccgcgcgcgc gctcgccaac
     4321 ccgggctgcc gccgcatggc gctgatcggc aacggcgcgc agagcgagtt ccaggcgctg
     4381 gccttccaca cgctgctcgg catcgacgag gtccgcgtgt tcgacgtcga cccggccgcc
     4441 accgacaagc tcgtgcgcaa cctcgccgca acgaaggggc tgcgcgtgat ccgcgcggcg
     4501 tcgacgcgcg aggcggtgcg cggcgcgcag atcgtcacga ccgtcactgc cgacaagacg
     4561 cgggcgacga tcctgacgcc ggagatgatc gaacccggca tgcacatcaa cgcggtcggc
     4621 ggcgattgcc ccggcaagac cgagctgcac gccgacgtgc tgcggcgcgc gcgcgtgatc
     4681 gtcgagtacg agccgcagac gcgcatcgag ggcgacatcc agcagctgcc gcccgacttc
     4741 ccggtgaccg agctgtggcg cgtgctgtcg ggcgcggccg cggggcgcga gcatgccggc
     4801 caggtgacgg tgttcgactc ggtcggcttc gcgctcgagg actactcggc gctgcgctac
     4861 gtgcacgaac tcgcgctggc ccgcggcgcc ggcgacgacg tcgcgctggt gccgccgggc
     4921 gcgaacccga aggacctgtt cggccgcctg gccgaacccg ccgacgccgc cgcgcgcgac
     4981 gcgctggcct cggtcctggg ggcgctgcgc cgcggccagc cggcatgagg tcgatgaagg
     5041 cgccgccgcc ggcaccgatg gttcgcggcg cctgatcggg ccgtcagacc tcgcgcagga
     5101 actgcagcag cagccgtgcg acctcggccg gctgctcctg ctgcacccag tggccggcgc
     5161 cttcgaccca gtcgcagccg cgcatccgcg tgcaggcctg gtcctgcatg ctctgaagcg
     5221 cgccgggcca ttgccacggg ccccagtcgc tgcggccggc gatgaagcgc gagggcacgt
     5281 cgatgtggcg ccccgcgaac aggcgcaggc cggcactgga cgtgccgtcg gtctggcagc
     5341 ggtaccactg cagcccgccc tggaagccgg tgcgcgcgaa ctcggcggcg tagaccgcga
     5401 gttcggcgtc gggcagccag cggcaggccg cgacctgcgc cggcgtcggc atctgcggcg
     5461 cgaccgtctc gctcatgccc ttgtgcaggt ccatcacgta gtagtcgggc agcttcgcga
     5521 actcggcggc gctgcgcgcg ctcagcgcga acggtcggtt gccgctccag tcggcgctct
     5581 tgaaatggta gtaggcgcgc aggaagtcgt gcaggccctg cgagcagtga cgcatctcgg
     5641 cgtcggcacc gcgctcggag tagtaccact ggtagtgctt gcgcggccgt ggcagccggg
     5701 ccagttcggc gcgccagtcg gctgcaggcg cgaccggcgc ggcgggcggt gcattcgcgg
     5761 tgtcgaccgg gaacgcgggc gggccgccga acggcgcgct catcagcacg acggcgcgga
     5821 acacgtcggg ccgcgccagc gcgcagcagg ccgccaccgg tgagccgaag tcgtggccga
     5881 cgaccgcggc gagcgatgcg tggcccatgg ccgcgacgag cccgagcgcg tcggtcgcca
     5941 ggttcagcag gcggaacgac gccaggtcgc cgtcgtagtc ggcgtcccac ccggtgctgc
     6001 ggccgtagcc gcgctggtcg ggcgcgaaca catggaagcc ggcctgcgcg agcggcagca
     6061 gcagcttgcg ccagctgtag gcgagctcgg gaaagccgtg cagcagcagc acgccgggcc
     6121 gcgacggatc gccggcctcg agcacatgca tctgcaggcc gttgccgttg tcgacgatgc
     6181 gcgcgcgaat gccgggcgcc agcggaagat cgggcagcgg gccgctcacg gcgcggcggc
     6241 ttcgggcggc aacgactcga gaaaggcgag cggcgcgcgg ttgaactcca ccggcttctc
     6301 cagccccggc aggtgcgcga cgccttccat cacgaccgag gcgacctgcg ggatgcgctg
     6361 cacgatctcg cgcatgcgcg cgcccagcgg cgcgaagtcc ggcgtgcccc acacgaccag
     6421 cgtcggcacg gcgatctctt cgagccgctc ccttttcttc atgcggcggc ctcctggtcc
     6481 tgcagcagcc gccacatcac cttgcccgag ccggacctgg gcagcgcgtc gacgaactgc
     6541 acgagcttgg gcaccttgta cgcggccatg ttctcgcgcg cccagttcgt gatgtcctcg
     6601 gcggtggtct tgcccttcgc ctcggcgcgc agcacgacca cggccttgac ggtctcgccg
     6661 cggtaggcgt cgcgcgcggc gatgatgcag gcctcctgca ccgccgggtg cttgtacagc
     6721 agcatctcga cctcggccgg ccagatcttg tagccgctcg cgttgatcat gcgcttgagg
     6781 cggtcggtca tgaagaagta gccttcctcg tccatgcggc cgaggtcgcc ggtgcggaag
     6841 aaggtctggc cctcgaactc gacgaaggcc tctctcgtcg cctcgggctg gccccagtag
     6901 cccttgaaca ccatcggccc gcggctgatg atctcgccga cctcgcccac cggcaattcc
     6961 ttcattgtga gtggatccac gacgcgcgaa tcgacgccga agaccgggat gcccaggcac
     7021 tgcagcttcg cgcgttcggg cgggttcgag tgcgtcggtg cggcggtctc ggtcaggccg
     7081 tagccctcgc agaaggtcag gccgtattcg tcgagcaggc gctgcgccac ggcctgcggc
     7141 atcgccgcgc cgccgccgct gaggtaggtc aggctggtca ggtcgaagct cttgtagttc
     7201 gggctgccga acaggtcgat gatcatcgtc gggatgcagg tccagtgcgt gaccttgtat
     7261 ttcgagatca accggcccgc gagttcgcgg tcccagcgcg gcaggatcac gctggtgcag
     7321 ccgatcgcga tgttgccaag cacgtagtag agcatgccgg tgatgtggaa catcggcacg
     7381 accgcgaggc tgacagtctc ggcgctcgcg t
//
LOCUS       DATMJB010000188         5548 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_211749, whole genome shotgun sequence.
ACCESSION   DATMJB010000188 DATMJB010000000
VERSION     DATMJB010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211749"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /gene="hpnC"
                     /locus_tag="VNU48_04245"
     CDS             complement(<1..613)
                     /gene="hpnC"
                     /locus_tag="VNU48_04245"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HWI10518.1"
                     /translation="MSVDHYENFPVASILCPPALRPAVTAIYAFARTADDLADEGDAT
                     PQQRLAELAAYRADLDAACRGAALSARWPQVFGALRPVLARHRLPPDLLTDLLSAFEQ
                     DVVKHAYADRAELLDYCRRSANPVGRLLLQLYGVDGPAALRQSDAICSALQLANFWQD
                     LGVDTRRGRLYAPAGDCTRHGVDPQSLLAGQDSPTVRALVADLV"
     gene            791..1933
                     /locus_tag="VNU48_04250"
     CDS             791..1933
                     /locus_tag="VNU48_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWI10519.1"
                     /translation="MRHALLITLTSVAILGACSKTEPAPEPVRAVRTVTISPQTAGGA
                     YEYAGEVKARTESRLSFRVSGKMVKRLVDLGDTVKAGQMLAQLDASDLRLGQDAARAT
                     MAAAQANYDQSAADFKRYKDLADKGFIGPAELDRREMAAKTARAQLDQARAQASVQGN
                     QAGYASLVADASGVITGVELEPGMVASAGTPVLRLAHEGPRDVVFSVPEDKVATVKAL
                     AGQPGRFSVRLWGSSDAPLPATIREISAAADPTTRTFLVKADIGPIAAGGVRLGQTAT
                     VLMELPQTVGVTKLPLSALREEQGRSAVWVVDRASMTVKSQEVKLAGANGNEAVVASG
                     LAPGQIVVTAGVHVLSPGQKVKFYIDPGMPSASSASAPLVTPVGVK"
     gene            1945..5091
                     /locus_tag="VNU48_04255"
     CDS             1945..5091
                     /locus_tag="VNU48_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWI10520.1"
                     /translation="MGTSPRAADTASRFNISKWALDHSALTRYLLVVLLLVGAAAYFQ
                     LGQDEDPPFTFRAMALRVFWPGATAQQVAEQVTDKLERTLQEVPFADRIRSYSKPGEA
                     LIIFQVRDSSPPKEMQQIWYTVRKKIGDMRATLPGGVIGPYFNDEFGDVYGSIYALSA
                     DGFTYQELKDQADRVRQRLLKVQDVNKVAIFGAQDEKLFVEISQKRLAQLGLDFTQVL
                     AQIGQQNAVESAGTITAPTDFVQVRVGGQFNSVDELRQFPIRAAGASFKLGDIAEIRR
                     GYVDPPQVKVRHQGHEVIALGISMAKGGDIVSMGKALTVAIAQIRAELPAGIELHQFQ
                     NQSKVVTTSVNEFVGTLIEAVVIVLGVSFISLGLHFKPRSFKFTLDWRPGMVVGITIP
                     LVLAITFVTMYYWGVGLHKISLGSLIIALGLLVDDAIISVEMMVRKLEEGYDKVRAAT
                     FAYDATAMPMLTGTLITAVGFLPIGLAQSAVGEYTFAIFAVTAAALLISWFVSVYFVP
                     YLGVRLLKTPPHAAGEQPHELFDTPFYARFRATVNWCVQHRWITIAATIGTLMLGVAG
                     MGQVQEQFFPDSSRPEIIVDLWYPEGTSYAANEAVTKRVEARIMKLDGVDNVTTWVGS
                     GVERFILVLDQVFPQSNVSQMVVMPKSPAARERLRKLLPVLLATEFPEVRGRAKLLPN
                     GPPVAYPVQFRVVGPDPAKVRAYADEVKAIMRKNPNLRGINDNWNESIKTLRLDVDQD
                     KARALGVTSQSIAQAARTSNTGTTIGQYREDDKLVDIVLRQPLEERNQITDLANAYVP
                     TSAGRNIPLGQIARANFTWEPGVLWREGRDYAATVQGDIVDGLQGATVTAQLDPLFAP
                     VRARMLDGYRIEVAGAVEESSKGQGSIAVGVPLMLFFMFTLLMLQLHSFSRSVLVFLT
                     APLGLAGIAAALLVLNRPFGFVALLGVIALMGMIMRNSVILIDQIEQDRAKGVPTWNA
                     IVEAAVRRFRPIMLTAAAAVLEMIPLSRSVFWGPMAVAIMGGLIVATVLTLLSLPAMY
                     AAWFRVKPPGGEKAAATS"
     gene            5170..5256
                     /locus_tag="VNU48_04260"
     tRNA            5170..5256
                     /locus_tag="VNU48_04260"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5204..5206,aa:Leu,seq:tag)
     gene            5286..>5548
                     /locus_tag="VNU48_04265"
     CDS             5286..>5548
                     /locus_tag="VNU48_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HWI10521.1"
                     /translation="MTVQVETLDKLERRITLLLPTATISSEVESRLKKLSRTVKADGF
                     RPGKVPMSVVTQRYGYSVHYEVMNDKVGQVFSEAVNEAKLRVAG"
BASE COUNT          901 a         1826 c         1961 g          860 t
ORIGIN      
        1 cgacgaggtc ggcgaccagc gcgcgcacgg tcggcgagtc ctggcccgcc agcagggact
       61 gcggatcgac gccgtggcgc gtgcagtcgc cggccggcgc gtagagccgg ccgcgccgcg
      121 tgtcgacgcc gaggtcctgc cagaagttcg cgagctgaag cgcgctgcag atcgcatccg
      181 actggcgcaa cgcggccggg ccgtcgacgc cgtacagctg cagcagcaag cgcccgacgg
      241 ggttcgccga gcggcggcag tagtcgagca actcggcgcg gtcggcataa gcgtgcttga
      301 ccacatcctg ctcgaacgca ctgagcaggt cggtcagcaa gtcgggcggc agccggtgtc
      361 gcgcgagcac cgggcgcagc gctccgaaga cctgcggcca gcgcgccgac agggctgcgc
      421 cccggcatgc cgcgtcgaga tcggcgcggt aggcggcgag ctcggcgagc cgctgctggg
      481 gcgtcgcatc gccttcgtcg gcgaggtcgt ccgcggtgcg cgcgaaggcg taaattgccg
      541 tcaccgccgg tcgcaacgcg ggcgggcaca gaatggatgc gacggggaag ttttcgtagt
      601 ggtcgacgct cacggagggc gattgtccac gttgttcggg catgcgccgg gcacgcaggg
      661 tttgacacgg gggagcgccg cgaatatatt actgaccagt cagtcatttg cagtatgcgg
      721 gcttgtgcgg cgcccgccac ggctcggcga cgccctgcgg ccgtcgccgg aacgcacgtc
      781 aaggaacgcc atgcgccatg cgcttctcat caccctgacc agcgtcgcga ttctcggcgc
      841 ctgctcgaag accgaacccg cgccggagcc ggtgcgcgcc gtgcgcaccg tgacgatctc
      901 gccgcagacc gccggtggtg cctacgaata cgccggcgag gtcaaggcac gcaccgagtc
      961 gcgactgagc tttcgggtca gcggcaagat ggtcaagcgc ctggtcgacc tgggcgacac
     1021 cgtgaaggcc ggccagatgc ttgcacagct cgacgccagc gacctgcggc tcgggcagga
     1081 tgcggcgcgc gcgacgatgg cggccgcgca ggccaactac gaccagagcg cggccgactt
     1141 caagcgctac aaggacctgg ccgacaaggg cttcatcggc ccggccgaac tcgaccgccg
     1201 cgagatggcg gcgaagacgg cgcgcgccca gctcgatcag gcgcgggcgc aggccagcgt
     1261 gcagggcaat caggccggct acgcgtcgct ggtcgccgat gcgagcggtg tgatcactgg
     1321 cgtcgaactg gagccgggca tggtcgcctc ggccggcacg ccggtgctgc gcctggcgca
     1381 tgaggggccg cgcgacgtgg tgttctcggt gcccgaggac aaggtcgcga cggtcaaggc
     1441 actcgcaggg cagccgggcc gcttcagcgt gcgcctgtgg ggcagctcgg acgcaccgtt
     1501 gccggcgacg atccgcgaga tttccgcggc ggccgacccg acgacgcgca ccttcctcgt
     1561 caaggccgac atcggcccga tcgcggccgg cggcgtgcgc ctggggcaga ccgccacggt
     1621 gctgatggaa ctgccgcaga ccgtgggcgt gaccaagctg ccgctgtcgg ccttgcgcga
     1681 ggagcagggc cgcagcgcgg tctgggtggt cgatcgcgcc agcatgacgg tgaaatcgca
     1741 ggaggtgaag ctggccggcg ccaacggcaa cgaggcggtg gtcgcgtccg gcctcgcgcc
     1801 gggtcagatc gtcgtgacgg ccggcgtgca cgtgctcagc ccggggcaga aggtgaagtt
     1861 ctacatcgac cccggcatgc catcggcctc gagcgcttcg gcgccgctgg tcacgccggt
     1921 cggcgtcaag tgagcggccc gatcgtgggg acgtcgccgc gcgcggccga cacggcgtcg
     1981 cgcttcaaca tctcgaagtg ggcactcgac cattcggcgc tgacgcgcta cctgctggtc
     2041 gtgctgctgc tggtcggggc cgccgcgtac ttccagctcg gccaggacga ggacccgccg
     2101 ttcaccttcc gcgcgatggc cttgcgcgtg ttctggcccg gcgccactgc gcagcaggtc
     2161 gccgagcagg tcaccgacaa gctcgagcgc acgctgcaag aggtgccctt cgccgacagg
     2221 atccgcagct actccaagcc gggtgaggcg ctgatcattt ttcaggtgcg cgactcgtcg
     2281 ccgccgaagg agatgcagca gatctggtac acggtgcgca agaagatcgg cgacatgcgc
     2341 gcgaccttgc ccggtggcgt gatcgggccg tatttcaacg acgagttcgg cgatgtctac
     2401 ggctcgatct acgcgctctc ggccgacggt ttcacctacc aggaactgaa ggatcaggcc
     2461 gaccgcgtgc gccagcgcct gctgaaggtg caggacgtca acaaggtcgc gatcttcggc
     2521 gcgcaggacg agaagctgtt cgtcgagatc tcgcaaaagc gcctggcgca actcggcctg
     2581 gacttcacgc aggtgctggc gcagatcggc cagcagaacg cagtcgagtc ggccggcacg
     2641 atcaccgcgc cgaccgattt cgtgcaggtg cgcgtcggcg ggcagttcaa ttcggtggat
     2701 gaactgcgcc agtttccgat ccgtgccgca ggggcgagtt tcaagctcgg cgacatcgcg
     2761 gagatccggc gcggctacgt cgacccgccg caggtcaagg tgcgccacca gggccacgag
     2821 gtcatcgcgc tgggcatctc gatggccaag ggcggcgaca tcgtcagcat gggcaaggcg
     2881 ctgacggtgg cgatagccca gatccgcgcc gagctgccgg ccggcatcga gttgcatcag
     2941 ttccaaaacc agtccaaggt cgtgacgacg tcggtcaacg agttcgtcgg cacgctgatc
     3001 gaggcggtcg tgatcgtgct cggcgtgagc ttcatcagcc tcgggctgca cttcaagccg
     3061 cgctctttca agttcacgct cgactggcgg ccgggcatgg tcgttggcat cacgatcccg
     3121 ctggtgctgg cgatcacctt cgtgacgatg tactactggg gcgtgggcct gcacaagatc
     3181 tcgctgggct cgctgatcat cgcgctcggc ctgctggtcg acgacgcgat catctcggtc
     3241 gaaatgatgg tgcgcaagct cgaggagggc tacgacaagg tgcgcgctgc gaccttcgcc
     3301 tacgacgcga ccgcgatgcc gatgctgacc ggcaccctga tcaccgcggt gggcttcctg
     3361 ccgatcgggc tggcgcagtc ggccgtgggc gaatacacct tcgcgatctt cgcggtgact
     3421 gcggcggcgc tgctgatctc gtggttcgtg tcggtctact tcgtgccgta cctcggcgtc
     3481 cggctgctga agaccccgcc ccatgccgcc ggcgagcagc cgcacgagct gttcgacacg
     3541 ccgttctatg cgcgctttcg cgccaccgtg aactggtgcg tgcagcaccg ctggatcacg
     3601 atcgccgcga caatcggcac cctgatgctc ggcgtcgccg gcatggggca ggtgcaagaa
     3661 cagtttttcc cggactcgag ccgccccgag atcatcgtcg acctgtggta tccggagggc
     3721 acctcctacg ccgccaacga ggctgtgacc aagcgcgtcg aggcgcgcat catgaagctc
     3781 gacggcgtcg acaacgtgac gacctgggtc ggcagcggcg tcgagcgctt catcctcgtg
     3841 ctcgaccagg tgttcccgca aagcaatgtc agccagatgg tcgtgatgcc gaagtcgccg
     3901 gcagcacgcg agcggctgcg caagctgctg cccgtgctgc tcgcgaccga gttccccgag
     3961 gttcgcggtc gcgccaagct gctgccgaac gggccgccgg tggcgtatcc ggtgcagttc
     4021 cgcgtcgtcg ggccggaccc ggcgaaggtg cgcgcctacg ccgacgaggt gaaggccatc
     4081 atgcgcaaga acccgaacct gcgcggcatc aacgacaact ggaacgagtc gatcaagacg
     4141 ctgcgcctgg acgtcgacca ggacaaggcg cgcgcgctgg gcgtgaccag ccagtcgatc
     4201 gcgcaggcgg cgcgcaccag caacaccggc acgaccatcg gccagtaccg cgaggatgac
     4261 aagctggtcg acatcgtgct gcgccagccg ctcgaagagc gcaaccagat caccgacctc
     4321 gcgaacgcct acgtgccgac cagcgccggc cgcaacatcc cgctgggaca gatcgcgcgc
     4381 gccaacttca cctgggagcc gggcgtgctg tggcgcgaag ggcgcgacta cgccgcgacg
     4441 gtgcagggcg acatcgtcga cggcctgcaa ggtgcgacgg tgacggcgca actcgacccg
     4501 ctgttcgccc cggtgcgggc gcggatgctc gacggttacc gcatcgaggt cgccggcgcg
     4561 gtggaggaga gcagcaaggg ccaaggctcg atcgcggtcg gtgtgccgct gatgctgttc
     4621 ttcatgttca ccttgctgat gcttcagctg cacagcttca gccgctcggt gctggtgttc
     4681 ctgaccgcgc cgttggggct ggccgggatc gccgcggcgc tgctggtgct gaaccggccc
     4741 ttcggcttcg tcgcattgct cggcgtgatc gccctgatgg gcatgatcat gcgcaactcg
     4801 gtgatcctga tcgaccagat cgagcaggac cgggccaagg gtgtgccgac ctggaacgcg
     4861 atcgtcgagg cggcggtgcg gcgcttccgg ccgatcatgc tgacggccgc cgccgcggtg
     4921 ctggagatga tcccgttgtc gcgcagcgtg ttctgggggc cgatggcggt ggcgatcatg
     4981 ggcgggctga tcgtcgcgac ggtgctgacg ctgctgtcgc tgccggcgat gtacgcggcc
     5041 tggttccgcg tcaaaccacc cggtggcgag aaggctgccg cgaccagcta aaatccgcgg
     5101 ttcgccgaga aagccagggt ggcggggccg cgcggcggcc tcggcatctt gtcggccgga
     5161 aggccgtagg cgcgggtggc gaaattggta gacgcaccag gtttaggtcc tgacgccagc
     5221 aatggtgtgc gggttcgagt cccgccccgc gcaccagcat tgaaaatcca gagagtcctt
     5281 acatcatgac cgtccaagtt gaaaccctcg acaagctgga gcgccgcatc acgctgctgc
     5341 tgcccaccgc caccatcagc tcggaagtcg agtcgcgcct gaagaagctg tcgcgcaccg
     5401 tcaaggccga tggctttcgc cccggcaagg tgcccatgag cgtggtcacg cagcgctacg
     5461 gctactcggt gcattacgag gtcatgaacg acaaggtcgg ccaggtgttc agcgaggccg
     5521 tcaacgaggc caagctgcgc gtcgccgg
//
LOCUS       DATMJB010000189         3101 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_609476, whole genome shotgun sequence.
ACCESSION   DATMJB010000189 DATMJB010000000
VERSION     DATMJB010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609476"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..1056)
                     /gene="tolQ"
                     /locus_tag="VNU48_04270"
     CDS             complement(373..1056)
                     /gene="tolQ"
                     /locus_tag="VNU48_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HWI10522.1"
                     /translation="MNNDFSIFTLVTQASFVVQLVMAGLMIASLASWTVIFTKLFGLK
                     RIRNSNEDFEREFWAGKNINDLYTDAVKRTDGSPMERIFASGMREFMKLRERRVVDAG
                     ALLDGARRAMRASFQRELDTIEAHLSFLATVGSVSPYVGLFGTVWGIMHAFVGLANMT
                     QVTLATVAPGIAEALVATAIGLFAAIPAVIAYNRFARDIDRTAIQLETFIEEFSNILQ
                     RNAGAPAAH"
     gene            complement(1056..1505)
                     /gene="ybgC"
                     /locus_tag="VNU48_04275"
     CDS             complement(1056..1505)
                     /gene="ybgC"
                     /locus_tag="VNU48_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HWI10523.1"
                     /translation="MHASDPLPFRFQLRVYWEDTDAGGVVFYANYLKFFERARTEWLR
                     SFGHEQERMRTETGAVFIVTDTTLRYLSPARLDDLLAVTVQVKHAGRAQMTIAQQAWR
                     RSAQGETLLAEGTIRIGCVDAGTFRPQRIPNSIVETLSSSLTGTSLP"
     gene            complement(1515..1907)
                     /locus_tag="VNU48_04280"
     CDS             complement(1515..1907)
                     /locus_tag="VNU48_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HWI10524.1"
                     /translation="MKLFLRPSMTLALTVALGGCALPPPAPPGLMDVAERPAEKALLA
                     GMRAYDDAQYAQAEQALTQALRAGLASPRDRAAAHKHLAFIYCSSKRLPACEAEFRAA
                     RADDPGFALSKAEAGHPLWGPVWQRVRQ"
     gene            complement(1910..>3101)
                     /locus_tag="VNU48_04285"
     CDS             complement(1910..>3101)
                     /locus_tag="VNU48_04285"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWI10525.1"
                     /translation="AYIAMELLKGRDLRQLRQEGWRATPAQAALIVRRVADALGYAHS
                     KGVVHRDVKPANIFMIGRTQPKVLDFGIARIAHQHDAPHSQGGDIAAGSPYYMAPEQV
                     RHEPVDRRADVFSLGVVLYELLTDLKPFRGNTLAEITDAVLEHDPPFAHLVDPNVPEA
                     LAVICARALSKNPEHRFRSARAFSRELRHWLDENTVASEGGDVRVGAAAPRGRPLWLA
                     LAGFVTLALGTTAWLLLGAQRAAAPTAPITPPATLAATPVLAVAAAVVDAGASSVPSV
                     NAMPASAAALPQAPPLVREAKDSSKERRAREAREREARLAVAAPQPRPAAPAATGIVR
                     VAISPWGEVEVDGKPAGTSPPLTELSLSAGRHQIVVRNADLPSYSSAVNISADQPVTV
                     KHKF"
BASE COUNT          441 a         1049 c         1141 g          470 t
ORIGIN      
        1 ccgtgcacgt attggcgcgg gatcggaccg gcggggcatg ctgctgcgct catgtccccc
       61 ggggtcggcc tgcggccaac ccctcctcct ttacttcgct ccgcagcacg ccccaccggc
      121 ccgatctgct ccaccgtgca gcccgatgac cggcgcacaa cacctcggtc tgctgccgag
      181 agagtcgggt gggcaacgca gcgaagtaaa ggaggagggg cgggcgcagc ccgttccggg
      241 ggacatgagc ggagttgtcc acccgactct ctcggctcgc gccgaccgtc aaagggcgca
      301 cgctcgcttg cgaacgaccg caacgggccg caagccgcca caccgccaca actccatgtt
      361 gccaagttca tttcagtggg cggccggcgc gccggcgttg cgctgcagga tgttcgagaa
      421 ctcctcgatg aaggtctcga gctggatcgc ggtgcggtcg atgtcgcgcg cgaagcggtt
      481 gtaggcgatc accgcgggga tcgcggcgaa caggccgatc gcggtggcca ccagcgcctc
      541 ggcgataccg ggcgccaccg tcgcgagcgt cacctgcgtc atgttggcca ggccgacgaa
      601 ggcatgcatg atcccccaca ccgtgccgaa caggccgacg tagggcgaga ccgagccgac
      661 cgtggcgagg aacgacaggt gcgcctcgat cgtgtcgagc tcgcgctgga agctcgcgcg
      721 catcgcgcgg cgcgcgccgt cgagcagcgc gccggcatcg acgacgcggc gctcgcgcag
      781 cttcatgaac tcgcgcatgc ccgaggcgaa gatgcgctcc atcggcgagc cgtcggtgcg
      841 cttcaccgca tcggtgtaca ggtcgttgat gttcttgccg gcccagaact cgcgctcgaa
      901 gtcttcgttc gagttgcgga tgcgcttcag gccgaacagc ttggtgaaga tcacggtcca
      961 gctcgcgagc gaggcgatca tcaacccggc catcaccagc tggaccacga agctcgcctg
     1021 ggtgaccagc gtgaagatcg agaagtcgtt attcatcagg ggaggctggt gccggtgagg
     1081 gaagaagaga gcgtttcgac gatcgaattc ggaatgcgct gcggcctgaa agttccggcg
     1141 tccacgcagc cgatgcggat cgtgccttcg gcgagcaagg tctcgccttg tgcggatcgg
     1201 cgccaggcct gctgcgcgat cgtcatctgc gcgcggccgg catgcttgac ctggaccgtg
     1261 accgcgagca ggtcatcgag ccgcgccggg ctcaggtagc gcagcgtcgt gtcggtgacg
     1321 atgaagaccg cgccggtctc ggtgcgcatg cgttcctgtt cgtggccgaa cgagcgcagc
     1381 cactcggtgc gcgcgcgctc gaagaacttc aggtagttgg cgtagaagac cacgccgccg
     1441 gcgtcggtgt cttcccagta gacgcgcagc tgaaatcgga agggcagtgg gtcgcttgca
     1501 tgcatcggcc ccgattattg ccgcacccgc tgccacaccg gcccccacag cggatgcccg
     1561 gcctcggcct tgctcagcgc gaagcccggg tcgtcggcgc gcgcggcgcg gaactcggct
     1621 tcgcaggccg gcagccgctt gctgctgcag tagatgaacg cgaggtgctt gtgcgcggcg
     1681 gcgcggtcgc gcggcgaggc caggccggcg cgcagcgctt gcgtcagcgc ctgttcggcc
     1741 tgcgcgtact gggcgtcgtc gtaggcgcgc atgccggcga gcagggcctt ctcggccggc
     1801 cgttcggcga cgtccatcag gcccggcggt gccggtggcg gcaatgcgca gccgccgagc
     1861 gccacggtga gcgcgagcgt catcgaaggg cgcagaaaga gtttcatggt cagaacttgt
     1921 gcttgacggt gacgggctgg tcggcgctga tgttgaccgc gctgctgtag ctcggcaggt
     1981 cggcgttgcg cacgacgatc tggtgccggc ccgcgctcag gctgagttcg gtcagcggcg
     2041 gcgaggtgcc cgcgggcttg ccgtcgacct cgacctcgcc ccaggggctg atcgcgaccc
     2101 gcacgatgcc ggtggccgcg ggcgctgcgg gccggggctg cggcgcggcg acggcgagcc
     2161 gggcctcgcg ttcgcgtgcc tcgcgggcac gccgttcctt ggacgaatcc ttcgcctcgc
     2221 gcaccagcgg cggcgcctgg ggcagcgctg cggccgaggc cggcatggcg ttgacgctcg
     2281 gcacgctcga cgcgcccgcg tcgaccaccg ccgctgccac cgcgagcacg ggtgttgccg
     2341 cgagcgtggc cggtggcgtg atcggcgctg tcggcgcggc cgcgcgctgc gccccgagca
     2401 gcagccaggc cgtcgtgccg agggccagcg tgacgaagcc ggcgagcgcg agccacagcg
     2461 gccggccgcg gggtgcggcg gcgccgaccc gcacgtcgcc gccctccgac gcgacggtgt
     2521 tctcgtcgag ccagtggcgc agttcgcgcg agaacgcgcg cgccgagcgg aaccggtgct
     2581 ccgggttctt cgacagcgct cgggcgcaga tcaccgccag cgcctcgggc acgtttggat
     2641 cgacgaggtg cgcgaacggc gggtcgtgct cgagcaccgc gtcggtgatt tcggcgagtg
     2701 tgttgccgcg gaacggcttc aggtcggtca gcagctcgta cagcacgacg ccgagcgaga
     2761 acacgtcggc acgccggtcg accggctcgt ggcgcacctg ctcgggcgcc atgtagtagg
     2821 gcgagccggc cgcgatgtcg ccgccctggc tgtgcggcgc gtcgtgctgg tgggcgatgc
     2881 gcgcgatgcc gaagtccagc accttgggct gggtgcgacc gatcatgaag atgttggccg
     2941 gcttcacatc gcggtgcacc acgcccttgc tgtgcgcgta gccgagcgcg tcggcgacgc
     3001 ggcgcacgat cagcgcggcc tgcgccggcg tggcgcgcca gccctcctgg cgcagctggc
     3061 gcaggtcgcg gcccttcagc agctccatcg cgatgtaggc g
//
LOCUS       DATMJB010000190         3725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_790840, whole genome shotgun sequence.
ACCESSION   DATMJB010000190 DATMJB010000000
VERSION     DATMJB010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790840"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VNU48_04290"
     CDS             complement(<1..993)
                     /locus_tag="VNU48_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWI10526.1"
                     /translation="MLDASSPPHPAPLSREADTTLTEQLAGRFKDRIQQRLLAPGARL
                     PSVRECARRHRVSPHTVVAAYDQLLALGLVEARKQRGFFVRDGAAARPTAPGAPATDR
                     ASAHPLPVSATSLIRGMFQPPGAWPMPGLGTLPADWLDLPLLAGALRKVSSGAQLGAV
                     SLQYGEPAGEGRLRRALAAKLTDFGVRASPEQIITTIGATQALDVVTRTLVRAGDSVL
                     VDEPGWSVEYARLAALGLRVLPVPRGEDGPDLAVMQRLIEAQKPAQRPRLYVTVSVLN
                     NPTGASLSLQAAHRVLQLAQANDLHIVEDDTYAHLAPAHLPRMAALDALERTIYI"
     gene            1091..1243
                     /locus_tag="VNU48_04295"
                     /pseudo
     CDS             1091..1243
                     /locus_tag="VNU48_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
     gene            1330..2589
                     /locus_tag="VNU48_04300"
     CDS             1330..2589
                     /locus_tag="VNU48_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWI10527.1"
                     /translation="MTARLVNAAPWQPARRAERMNPSVIREILKVTERPGIVSLAGGL
                     PSPDSFPVDAMREATARVLRDHPREALQYAASEGFAPLREWVAQQMATQGLAVDASQV
                     LITTGSQQGLDLTAKILIDAGSTVAVESPTYLGALQAFAPYEPEVVTVPCDDDGPLSE
                     GLGIAKEGPATAAPSATSGRGARFGEARPKPRFLYMLPNFQNPSGRCMSERRRAALVD
                     AARASGLPIVEDNPYGDLWFDAPPPPSLASRWPEGTVYLGSFSKVLAPGLRLGYVIAP
                     PALCPKLLQAKQAADLHTPGFNQRVVYEVIRDGFLRDHVPTIRARYKAQRDAMRAALE
                     KHLPAGCHWNVPSGGMFFWVELPEGVDATALLPKAVELGMAYVPGAAFFAADPKPNTL
                     RLSFVTVAPAEIERGVALLAQALKDAS"
     gene            2709..3497
                     /locus_tag="VNU48_04305"
     CDS             2709..3497
                     /locus_tag="VNU48_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HWI10528.1"
                     /translation="MLGNPLAVVHDAQDLSDTQMAAFARWTNLSETTFLLPPTDPTAD
                     YRVRIFTPHRELPFAGHPTLGSCHAWLEAGGVPRGSDEVVQQCAVGLIRLRRSHARLA
                     FAAPPCVRSGPLEPALLTRIVAALGLRADAVLDHQWVDNGPGWCAIRLASAEAVLALR
                     PDWAALAGIKLGVVGAHRADHDAQFEVRALIGEGPGYEDPVTGSLNASLAQWLIGSGV
                     APAAYVAAQGARLARRGRVFIEQIDGQIWVGGYSVSCVRGEVRL"
     gene            complement(3527..>3725)
                     /locus_tag="VNU48_04310"
     CDS             complement(3527..>3725)
                     /locus_tag="VNU48_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10529.1"
                     /translation="VDIALPPDHKSKPSVALVAISATLLGLLLSSAFVVMRRYAAWSR
                     EQFPQDAAARSALARAWRWRG"
BASE COUNT          493 a         1356 c         1373 g          503 t
ORIGIN      
        1 gatgtagatc gtgcgctcca gcgcatcgag cgcggccatg cgtggcaggt gcgcgggcgc
       61 gaggtgcgcg taggtgtcgt cctcgacgat gtgcaggtcg ttcgcctggg cgagttgcag
      121 cacgcggtgc gcggcctgca gcgacaacga cgcgccggtc gggttgttga gcaccgagac
      181 cgtgacgtac aggcgcggcc gctgcgccgg cttctgcgcc tcgatcaggc gctgcatcac
      241 ggccaggtcg gggccgtcct cgccgcgcgg caccggcagc acgcgcaggc ccagcgccgc
      301 cagccgcgcg tactcgaccg accagccggg ctcgtcgacc agcacgctgt cgccggcgcg
      361 caccagggtg cgcgtcacga catcgagcgc ctgggtcgcg ccgatcgtcg tgatgatctg
      421 ctcgggcgac gcgcgcacgc cgaagtcagt cagcttggcc gcgagcgcgc ggcgcagccg
      481 gccttcgccc gcgggctcgc cgtactgcag cgacaccgcg ccgagctgcg cgccgctcga
      541 gaccttgcgc agcgcgccgg ccagcagcgg caggtcgagc cagtcggccg gcaaggtgcc
      601 caggcccggc ataggccacg cgccaggggg ttggaacatg ccgcggatca gcgaggtggc
      661 gctgaccggc agcggatgcg cgctggcgcg gtcggtggcc ggtgcccccg gcgcggtggg
      721 gcgcgccgcc gcgccgtcgc gcacgaagaa gccgcgctgc ttgcgcgcct cgaccagccc
      781 gagcgcgagc agctggtcgt aggccgcgac cacggtgtgc gggctgaccc ggtggcggcg
      841 cgcgcactcg cgcaccgacg gcagccgtgc gccgggcgcc agcaggcgct gctggatgcg
      901 gtccttgaag cggccggcaa gctgctcggt cagcgtggtg tcggcctcgc gcgacagcgg
      961 ggccgggtga ggcggggaag acgcgtccag catggtgctc gttgtactgc caagatgacc
     1021 gatacagagg cgccggtcgt gtcgtcaagt gtactgtctc tgtgctggtg ttgaggcata
     1081 gactgcgggc atgacgaaga cgaacccgcg cgacgaaacc cgtggcctgt ggctcgggct
     1141 gctgggcgtc gtgatcttcg cgatgaccct gccgatgacg cgcctcgcgg tcggcccggc
     1201 cagcgacccg cagctggcgc cgctgttcgt caccgccggg cgcttctccc gccagcggcc
     1261 gtcgaacggc ttctgggcct gcgccgtgct cggctgcgcg atggccattc cccaacctcg
     1321 gaacccgtga tgaccgctcg ccttgtcaac gccgcgccct ggcagcccgc gcgccgcgcc
     1381 gagcgcatga acccgtcggt gatccgcgag atcctgaagg tcaccgagcg gcccggcatc
     1441 gtctcgctgg ccggcgggct gccgtcgccg gacagcttcc ccgtcgacgc gatgcgcgag
     1501 gccaccgcgc gcgtgctgcg cgaccacccg cgcgaggcgc tgcaatacgc cgccagcgaa
     1561 ggcttcgcgc cgctgcgcga atgggtcgcg cagcagatgg cgacgcaggg cctggcggtc
     1621 gacgcgtcgc aggtgctgat cacgaccggc tcgcagcagg ggctggacct gaccgccaag
     1681 atcctgatcg acgccggcag cacggtcgcg gtcgaatcgc ccacctacct gggcgcgctg
     1741 caggccttcg cgccctacga gcccgaggtc gtcaccgtgc cctgcgacga cgacggcccg
     1801 ctgtccgagg gcctgggcat cgccaaggaa gggccagcga ctgctgcccc ttccgcgacc
     1861 tccggtcgcg gcgcgcgctt tggcgaagct cggccgaagc ctcgctttct gtacatgctg
     1921 ccgaacttcc agaacccgag cgggcgctgc atgagcgagc gccggcgcgc tgcgctggtc
     1981 gacgcggcgc gcgcatcggg attgccgatc gtcgaggaca acccgtacgg cgacctgtgg
     2041 ttcgacgcac ccccgccgcc gtcgctggcg tcgcgctggc ccgagggcac ggtgtacctc
     2101 ggctcgttct ccaaggtgct ggcgccgggg ctgcgcctgg gctacgtgat cgcgccgccg
     2161 gcgctgtgcc cgaagctgct gcaggccaag caggccgccg acctgcacac gccgggcttc
     2221 aaccagcgcg tcgtctacga ggtgatccgc gacggcttcc tgcgcgacca cgtgccgacg
     2281 atccgcgcgc gctacaaggc gcagcgcgat gcgatgcgcg cggcactcga gaagcacctg
     2341 ccggcgggct gccactggaa cgtgccgagc ggcggcatgt tcttctgggt cgagctgccc
     2401 gagggcgtgg acgccaccgc gctgctgccg aaggcggtcg aactcggcat ggcctacgtg
     2461 cccggcgccg cgttcttcgc cgccgacccg aagccgaaca cgctgcgcct gtcgttcgtg
     2521 acggtggcgc cggccgagat cgagcgcggc gtcgccttgc tggcgcaggc gttgaaggac
     2581 gcgagttgac gcgggccgag cgccgggccg ctcccaagcc ggcccgcatc ccctcggggg
     2641 atcgaccgac gtacccgtcg ggcgaggggc gaacattcac gcaggtcgac gtttttaccg
     2701 acacgccgct gctgggcaac ccgctcgccg tggtgcacga tgcgcaggac ctgagcgata
     2761 cgcagatggc ggcattcgcg cgctggacca acctcagcga gacgaccttt ctgctgccac
     2821 cgaccgaccc gactgccgac taccgcgtgc gcatcttcac gccgcaccgc gagttgccgt
     2881 tcgccggcca cccgaccctg ggcagctgcc acgcctggct cgaggccggc ggcgtgccgc
     2941 gcggcagcga cgaggtggtg cagcagtgcg cggtcggact gatccggctg cgccgcagcc
     3001 acgcacgcct cgcgttcgcg gcgccgccct gcgtgcgcag cgggccgctc gagcccgcgc
     3061 tgctgacgcg catcgtcgcg gcgctgggac tgcgcgccga cgccgtgctc gatcaccagt
     3121 gggtcgacaa cggcccgggc tggtgcgcga tccgcctcgc gtcggccgag gccgtgctgg
     3181 cgctgcgccc cgactgggcc gcgctggccg gcatcaagct cggcgtcgtc ggcgcgcatc
     3241 gcgcggacca cgacgcgcag ttcgaggtgc gcgcgctgat cggcgaaggc ccgggttacg
     3301 aagatcccgt gaccggcagc ctgaacgcca gcctcgcgca atggctgatc ggcagcggcg
     3361 tcgcgccggc cgcgtacgtc gccgcgcagg gcgcgcgcct ggcgcgtcgc ggtcgtgtgt
     3421 tcatcgaaca gatcgacggc cagatctggg tcggcgggta cagcgtcagc tgcgtgcgcg
     3481 gcgaggtccg cctctgaagg gtggacgctg cagcgcggcg gcggtgtcag ccgcgccagc
     3541 gccaggcccg cgccagcgcg ctgcgcgccg cggcgtcctg cgggaactgc tcgcgcgacc
     3601 acgcggcgta gcggcgcatc acgacgaacg cgctcgacag cagcagcccc agcagcgtgg
     3661 ccgagatcgc gaccagcgcg accgacggct tggacttgtg gtccggcggc agcgcgatgt
     3721 cgacc
//
LOCUS       DATMJB010000191         2788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_728372, whole genome shotgun sequence.
ACCESSION   DATMJB010000191 DATMJB010000000
VERSION     DATMJB010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728372"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VNU48_04315"
     CDS             complement(<1..877)
                     /locus_tag="VNU48_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HWI10530.1"
                     /translation="MRVLHVAAEIFPLVKTGGLADVLGALPQALIGQGADVRLLLPGL
                     PAIADAVLHQKIVGEIGPSFGAARITLRLGQMPYSRVPAYVIDAPFLYRRPGSPYQAS
                     DGSEWPDNAQRFALLGWVGAHLASGELDPSWQPELLHAHDWHAAMACAYLAAHPPASA
                     ASVFTVHNLAYQGLFPSGDFPLLGLSSRFMASTGLEYHQQVSFMKAGLKFAQRITTVS
                     PTYAREIGTHEFGFGLDGVIRGRGGDVSGVLNGVDGAVWDPATDTGIVARYSTAQLAG
                     KARCKQALQAELGLAP"
     gene            complement(874..2145)
                     /gene="glgC"
                     /locus_tag="VNU48_04320"
     CDS             complement(874..2145)
                     /gene="glgC"
                     /locus_tag="VNU48_04320"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HWI10531.1"
                     /translation="MTPDELALSLDLPKRAVALVLAGGRGSRLKSLTDRRAKPAVYFG
                     GKFRIVDFALSNCLNSGVRRIGVITQYKSHSLLRHLQRGWGFLKSEMNEFVDLLPAQQ
                     RTDDESWYRGTADAVYQNQDILASYGADYIVVLAGDHIYKMNYALMLADHVAKGRDCT
                     VGCIAVPRMEATAFGVMAVDENDLILDFVEKPVDPPAMPGDPDRALASMGIYIFNARF
                     LYKELERDINDPGSSHDFGKDIIPRAVKNGNAAAHPFALSAVGTRVNEEPYWRDVGTI
                     DAYWDANIDLTATDPLLNLYDTRWPIWTYQAQLPPAKFVHDQPDRRGMAIESVVSGGC
                     IVSGNVHRSVLFSSVRVHSYSTVSWSVLLPGAQVGRRARLTRAVIDRGCTIPDDMVIG
                     EDAALDAQRFHRSENGITLVTREMLDALIQA"
     gene            2357..>2788
                     /locus_tag="VNU48_04325"
     CDS             2357..>2788
                     /locus_tag="VNU48_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HWI10532.1"
                     /translation="MLNPNLQPSARSVATGIDEELLDHVGVMPADASPNDLMQAVSQV
                     ARHRLSQRWVRTQAEQADAKARRVYYLSMEFLMGRTLANALAALELRGEVAQAMDAHA
                     RKLEDVADQEPDAALGNGGLGRLAACFLDSMATLGLPSFGYG"
BASE COUNT          418 a          954 c          979 g          437 t
ORIGIN      
        1 gtggcgcgag gccgagttcg gcctgcagtg cctgtttgca gcgcgccttg ccggcgagct
       61 gggccgtgct gtagcgcgcc acgatgccgg tgtcggtggc ggggtcccag accgcgccgt
      121 cgacgccgtt cagcaccccg ctcacgtccc caccccggcc gcggatcacg ccgtccaggc
      181 cgaagccgaa ctcgtgcgtg ccgatctcgc gcgcgtaggt cgggctgacg gtggtgatgc
      241 gctgcgcgaa cttcaaaccg gccttcatga atgagacctg ctggtggtat tcgagcccgg
      301 tcgacgccat gaagcgcgac gacagcccca gcaacgggaa gtccccgctc gggaacagcc
      361 cttgatacgc gaggttgtgc acggtgaaca cgctcgcggc gctggccggc ggatgcgccg
      421 cgaggtaggc gcaggccatc gccgcatgcc agtcgtgggc gtgcagcagc tcgggctgcc
      481 aggacgggtc gagctcgccc gacgcgaggt gcgcgccgac ccagcccagc agcgcgaagc
      541 gctgcgcgtt gtcgggccac tcgctgccgt cgctggcctg gtacgggctg cccgggcggc
      601 ggtacaggaa cggcgcgtcg atcacatagg ccggcacacg gctgtacggc atctggccga
      661 gccgcagcgt gattcgcgcg gcgccgaagc tcgggccgat ctcgccgacg atcttctggt
      721 gcagcaccgc atcggcgatc gccggcagcc cgggcagcag caggcgcacg tcggcgccct
      781 gcccgatcag cgcctgcggc agcgcgccca gcacgtcggc caggccaccg gtcttgacga
      841 gcgggaagat ctcggccgca acgtgcagga ccctcatgct tggatcaagg cgtccagcat
      901 ctcgcgcgtc accagcgtga tgccgttctc cgagcggtgg aagcgctgcg cgtcgagcgc
      961 cgcgtcctcg ccgatcacca tgtcgtcggg aatggtgcag ccgcggtcga tcaccgcgcg
     1021 cgtcaggcgc gcccggcggc cgacctgcgc gcccggcagc agcaccgacc agctcaccgt
     1081 cgaatacgag tgcacgcgca cgctcgagaa cagcaccgag cggtgcacgt tgccagagac
     1141 gatgcaaccg cccgacacga ccgactcgat cgccatgccg cgccggtcgg gctggtcgtg
     1201 cacgaacttg gccggcggca attgcgcctg gtaggtccag atcggccagc gcgtgtcgta
     1261 caggttgagc agcgggtcgg tcgcggtcag gtcgatgttg gcgtcccagt aggcgtcgat
     1321 cgtgccgacg tcgcgccagt acggttcttc gttgacgcgc gtgcccaccg cgctgagcgc
     1381 gaacgggtgc gccgcggcgt tgccgttctt gaccgcgcgc ggaatgatgt ccttgccgaa
     1441 atcgtggctc gacccggggt cgttgatgtc gcgctcgagt tccttgtaga ggaagcgtgc
     1501 attgaagatg tagatgccca tgctcgccag cgcccggtcg gggtcgccgg gcatagccgg
     1561 cgggtcgacc ggcttctcga cgaagtccag gatcaggtcg ttctcgtcga ccgccatcac
     1621 gccgaatgcg gtcgcctcca tgcgcggcac cgcgatgcag ccgaccgtgc agtcgcgccc
     1681 cttggcaacg tggtcggcca gcatcagcgc gtagttcatc ttgtagatgt ggtcgccggc
     1741 gagcacgacg atgtagtcgg cgccatagct cgcgaggatg tcctggttct ggtagaccgc
     1801 gtcggcggtg ccgcggtacc agctctcgtc gtcggtgcgc tgctgcgcgg gcagcaggtc
     1861 gacgaactcg ttcatctcgc tcttcaggaa accccagccg cgctgcaagt ggcgcagcag
     1921 gctgtggctc ttgtactgcg tgatcacgcc gatgcggcgc acgcccgagt tcaggcagtt
     1981 cgacagcgcg aaatcgacga tccggaactt gccgccgaag tacaccgccg gcttggcgcg
     2041 ccggtcggtc aggctcttca ggcggctgcc gcggccgccg gcgagcacca gcgcgaccgc
     2101 gcgtttcgga aggtccagac tcagggcgag ttcgtcgggc gtcatcttgt cgtctcctcg
     2161 gttgaccatt gtggcggctc cgcacgcagc gcccagtgtg gcgaagttcg ggcccgcgcc
     2221 atactcgcgg gcttcccgaa tccgcgtcgg cacacgccgc gggatccggg cgcaaaaatt
     2281 gcgagcccgt gcgtgccggc cggtcgcacg atgcgacact gatcgcgacg gcgattttca
     2341 gagggtggat gatccaatgc tgaaccccaa tttacagcct tcggcgcgca gcgtcgccac
     2401 cggcatcgac gaagaactgc tcgaccatgt cggcgtgatg ccggccgacg cgagcccgaa
     2461 cgacctgatg caggcggtgt cgcaagtcgc gcgccaccgc ctgtcgcagc gctgggtgcg
     2521 cacccaggcc gagcaggccg acgccaaggc gcgccgcgtg tactacctgt cgatggagtt
     2581 cctgatgggg cgcacgctgg ccaacgcgct ggccgcgctc gagctgcgcg gcgaggtggc
     2641 gcaggcgatg gacgcgcacg cgcgaaagct cgaggacgtc gccgaccagg aacccgacgc
     2701 cgccctgggc aacggtggcc tcggtcggct ggcggcgtgc ttcctcgact cgatggccac
     2761 gctcgggctg ccctcgttcg gctacggc
//
LOCUS       DATMJB010000192         4255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_728141, whole genome shotgun sequence.
ACCESSION   DATMJB010000192 DATMJB010000000
VERSION     DATMJB010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728141"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VNU48_04330"
     CDS             <1..531
                     /locus_tag="VNU48_04330"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWI10533.1"
                     /translation="APIGFKNGTDGNIRIAIDAIQAAARPHHFLSVHKNGQVAIVETR
                     GNKDCHVILRGGKTPNYDAAHVAAACKEIEAAKLDCTLMVDCSHANSSKQHERQADVA
                     RDVAAQLKAGSHCIFGVMVESHLHAGAQKFSAGKDDPSKLEYGKSITDACIGWEHSLE
                     VLEVLSDAVTARKASR"
     gene            615..911
                     /locus_tag="VNU48_04335"
     CDS             615..911
                     /locus_tag="VNU48_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HWI10534.1"
                     /translation="MPHTLVSPLQRGFVAAALAALCLACAALPAPAELSAVEREAIEA
                     AFARADTNGDGKLSREEAQRLPEIAARFDELDRDHDGFLSFAEFVVGATPPEKQ"
     gene            complement(940..1515)
                     /locus_tag="VNU48_04340"
     CDS             complement(940..1515)
                     /locus_tag="VNU48_04340"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HWI10535.1"
                     /translation="MSNRLTQIATRTGDDGSTGLGDGTRVPKDHLRVQAMGDVDELNS
                     QLGVLLAEPLPDDVRELLVVIQHELFNLGGELSIPGYALLQEDAVLRLDEALAHYNAT
                     LPRLKEFILPAGTRSAALAHVGRTIARRAERAVVALTAAEAVRAEPRHFLNRLSDLLF
                     VLARVLNRANLDGLGGDDVYWHSERLARDAD"
     gene            1583..3004
                     /locus_tag="VNU48_04345"
     CDS             1583..3004
                     /locus_tag="VNU48_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWI10536.1"
                     /translation="MNAPLPAHLLPHLEPRPMPAAMLETLRVQFGTRCSTALAVREQH
                     GRDESPIDAPPPEAVVYCESTEDVAAVVRLADRYAVPVIPFGVGSSLEGHLLAVQGGV
                     SIDLSRMNAIVAVQPEDLTVTVQAGVTRTQLNNEIRHTGLFFPIDPGADASLGGMSAT
                     RASGTNAVRYGTMRENVLGLTVVTASGEVIHTGTRARKSSAGYDLTRLMVGSEGTLGV
                     MTEITLRLYPLPEAISAATCTFPSIDAAVRTTIQIIQMGIPIARCELLDVHAVRAVNR
                     HDKLTLTEAPMLLMEFHGSAASVAEQAATVQEIATEFGGSGFQWATTPEERTRLWAAR
                     HHAYFAGLQLKAGCRTVTTDTCVPISRLAECVTQASDEATAAGMMHYIVGHVGDGNFH
                     IAYLVDPAIPSERETAERLNNEVVQRAIRAQGTCSGEHGIGLHKMDFLVTEAGAGAVA
                     MMRTLKRALDPKNIMNPGKIFVL"
     gene            complement(3020..>4255)
                     /locus_tag="VNU48_04350"
     CDS             complement(3020..>4255)
                     /locus_tag="VNU48_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HWI10537.1"
                     /translation="NSDPCISYLMEENTTAMQALVIAHAAYGHNSFFKGNYLFKLWTD
                     ASSIIDYLVYSKNYIAECEERHGLGPVEELLDSCHALQNYGVDRYRRPSKLSFAEELV
                     RRKERLAHAQSQVNDIWRTLPKKPERTEDSAETKRFPAELQENILYFVEKNAPLLEPW
                     QREIVRIVRKIAQYFYPQRQTQVMNEGWATFWHHHLLNTMYDDGYLTDGVMIEWLKSH
                     TNVIYQPPVGHRAYSGINPYALGFAMYTDIKRICDNPTDEDREWFPNFAGQPWLPTLD
                     HAMRNFKDESFVGQYLSPKLMRDMRLFAVLDDENAEKLQVSAIHDPSGFRRLREALSR
                     QYDLSSREPDIQVWNVNLRGDRSLTLRHTRHNNRPLHDNAQEVLKHAARLWGFGVHLE
                     SVDAAGAVAQRLSVPAPTH"
BASE COUNT          679 a         1409 c         1492 g          675 t
ORIGIN      
        1 gcgccgatcg gcttcaagaa cggcaccgac ggcaacatca ggatcgcgat cgacgcgatc
       61 caggccgcgg cgcggccgca ccatttcctg tcggtccaca agaacggcca ggtcgcgatc
      121 gtcgaaaccc gtggcaacaa ggattgccac gtgatcctgc gcggcggcaa gacaccgaat
      181 tacgacgccg cgcacgtcgc cgccgcctgc aaggagatcg aggccgcgaa gctcgactgc
      241 acgctgatgg tcgactgcag ccacgccaac agcagcaagc agcacgagcg ccaggccgac
      301 gtggcccgcg acgtggccgc gcagctgaag gcaggcagcc actgcatctt cggcgtgatg
      361 gtcgaaagcc atctgcatgc cggcgcgcag aagttcagcg ccggcaagga cgacccatcg
      421 aagctggagt acggcaagag catcaccgat gcctgcatcg gctgggagca ctcgctcgag
      481 gtgctcgagg tgctgagcga cgcggtgacc gcgcgcaagg cttcgaggta accggcgtaa
      541 gggggcgcaa gagagctggt cggccgctgc gcgagtgggt caaatgccgg aggtctctgc
      601 ccggaaattc ctcgatgccc cacacgcttg tctcgccgct acagcgcggg ttcgttgccg
      661 ccgcgctggc ggcgctttgt ctggcctgcg ccgcgttgcc ggcgccggcc gaactgtcgg
      721 cggtcgagcg cgaggcgatc gaggccgcgt tcgcgcgtgc cgacacgaac ggcgacggca
      781 agctgtcgcg cgaggaggcg cagcgcctgc ccgaaatcgc cgcccgcttc gacgagctcg
      841 atcgcgacca cgacggcttc ctgtcgttcg ccgagttcgt ggtcggcgcg acgccgcccg
      901 aaaagcagtg acgtgatgtg atgcggcgtg cggcgcgggt cagtcggcgt cgcgcgcgag
      961 gcgttcgctg tgccagtaca cgtcgtcgcc gcccaggccg tcgaggttgg cgcggttgag
     1021 cacgcgggcc agcacgaaca gcaggtcgga caggcggttc aggaagtggc gcggttcggc
     1081 gcgcaccgcc tcggccgcgg tcagtgcgac caccgcgcgc tcggcgcgtc gcgcgatcgt
     1141 ccgcccgacg tgcgcgagcg cggcgctgcg ggtgccggcg ggcaggatga actccttcag
     1201 gcgtggcagc gtcgcgttgt agtgcgcgag cgcttcgtcc aggcgcagca cggcgtcttc
     1261 ctggagcagc gcgtagcccg ggatcgagag ctcgccgccg aggttgaaca gctcgtgctg
     1321 gatcaccacc agcaactcgc gcacatcgtc gggcaagggc tcggccagca acacaccgag
     1381 ctgcgagttc agctcgtcga catcgcccat cgcctgcacg cgcagatggt ctttggggac
     1441 acgtgtgccg tcgccgaggc cggtgctgcc gtcgtcgccg gtgcgggtgg cgatctgggt
     1501 caatcgattg gacatggagc ggatgctagc gccttggcac aatgaacgcc ggccacgccc
     1561 tgcgtggcca aggggacttg ccatgaatgc accgctgcct gcccacctgc tgccgcacct
     1621 cgagccgcgc ccgatgccgg ccgcgatgct cgaaacgctg cgggtgcagt tcggcacgcg
     1681 ctgctcgacc gcgctggcgg tgcgcgagca gcacggccgc gacgagtcgc cgatcgacgc
     1741 gccgccgccc gaggccgtgg tctactgcga aagcaccgaa gacgtggccg cggtcgtcag
     1801 gctggccgat cgttacgcgg tgccggtgat cccgttcggc gtcggttcct cgctcgaggg
     1861 ccatttgctg gcggtgcagg gcggcgtcag catcgacctg tcgcgcatga acgcaatcgt
     1921 cgcggtgcag cccgaggacc tgaccgtgac agtgcaggcc ggcgtgacgc gcacccagct
     1981 gaacaacgag atccgccaca ccgggctgtt cttcccgatc gacccgggag ccgacgcgag
     2041 cctgggcggc atgagcgcga cgcgtgcgag cggcacgaac gcggtgcgct acggcacgat
     2101 gcgcgagaac gtgctcgggt tgaccgtggt gaccgccagc ggcgaggtga tccacaccgg
     2161 cacgcgcgca cgcaagtcgt cggccggcta cgacctgacg cggctgatgg tcggcagcga
     2221 gggaacgctc ggcgtgatga ccgagatcac gctgcggctg tacccgctgc ccgaggccat
     2281 ctcggcggcg acctgcacct tcccgagcat cgacgcggcg gtgcgcacga cgatccagat
     2341 catccagatg ggcatcccga tcgcgcgttg cgaactgctc gacgtccacg cggtgcgcgc
     2401 cgtgaaccgg cacgacaagc tcacgctgac cgaggcaccg atgctgctga tggagttcca
     2461 cggcagcgcg gccagcgtcg ccgagcaggc cgcgacggtg caggagatcg cgaccgaatt
     2521 cggcggctcg ggcttccagt gggcgaccac gcccgaggag cgcacccggc tgtgggcggc
     2581 gcgccaccac gcctatttcg ccggcctgca gctcaaggcc ggttgccgca ccgtgacgac
     2641 cgacacctgc gtgccgatct cgcgcctggc cgaatgtgtg acccaggcct ccgacgaggc
     2701 caccgccgcg gggatgatgc actacatcgt cggccatgtc ggcgacggca acttccacat
     2761 cgcctatttg gtggacccgg ccattcccag cgagcgcgag accgcggaac ggctgaacaa
     2821 cgaggtcgtg caacgcgcga ttcgcgccca gggcacctgc agcggcgagc acggcatcgg
     2881 gctgcacaag atggacttcc tcgtcaccga ggccggcgcc ggcgcggtcg cgatgatgcg
     2941 cacgctcaag cgcgcgctcg acccgaagaa catcatgaac ccggggaaga tcttcgtgct
     3001 ctgagcgctg ggctcgtctt cagtgcgtcg gcgcgggcac cgagagccgc tgcgcgaccg
     3061 cgccggccgc gtccacgctt tccaggtgca ccccgaagcc ccacaaccgg gcggcgtgct
     3121 tcagcacctc ttgcgcgttg tcgtgcagcg gccggttgtt gtggcgcgtg tgacgcaggg
     3181 tcagcgaccg gtcgccgcgc aggttcacgt tccagacctg gatgtcgggc tcgcgcgagc
     3241 tcaggtcgta ctggcgcgac agcgcctcgc gcagccgccg gaagcccgac ggatcgtgga
     3301 tcgccgagac ctgcagcttc tcggcgttct cgtcgtccag caccgcgaac aggcgcatgt
     3361 cgcgcatcag cttcggtgaa aggtactggc cgacgaagct ctcgtccttg aagttgcgca
     3421 tggcgtggtc cagcgtcggc agccacggtt ggccggcgaa gttggggaac cactcgcggt
     3481 cttcgtcggt cgggttgtcg cagatgcgct tgatgtcggt gtacatcgcg aagcccagcg
     3541 catacgggtt gatgccactg tacgcgcggt gcccgaccgg cggctggtag atcacgttgg
     3601 tgtgcgactt cagccactcg atcatcacgc cgtcggtgag gtagccgtcg tcgtacatcg
     3661 tgttcagcag atggtgatgc cagaacgtgg cccagccttc gttcatcacc tgggtctggc
     3721 gctgcgggta gaaatactgc gcgatcttgc gcacgatgcg cacgatctcg cgctgccagg
     3781 gctcgagcag cggcgcgttc ttctcgacga agtacaggat gttctcttgc agctcggcgg
     3841 ggaagcgctt ggtctcggcg ctgtcctcgg tgcgctcggg cttcttcggc agcgtgcgcc
     3901 agatgtcgtt gacctgcgat tgcgcgtgcg ccagccgctc cttgcggcgc accagctcct
     3961 cggcgaacga gagcttgctc gggcgccggt agcggtcgac gccgtagttc tgcaaggcgt
     4021 ggcagctgtc gagcagttcc tcgaccgggc cgaggccgtg acgctcttcg cactcggcga
     4081 tgtagttctt cgagtagacg aggtagtcga tgatcgacga cgcatcggtc cagagcttga
     4141 agaggtagtt gcccttgaag aagctgttgt ggccgtaggc ggcgtgcgcg atcacgagcg
     4201 cctgcatcgc ggtcgtgttc tcctccatca ggtagctgat gcaggggtcg gagtt
//
LOCUS       DATMJB010000193         4063 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_37871, whole genome shotgun sequence.
ACCESSION   DATMJB010000193 DATMJB010000000
VERSION     DATMJB010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37871"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="VNU48_04355"
     CDS             complement(<1..1236)
                     /locus_tag="VNU48_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWI10538.1"
                     /translation="MSAIAAAAADQTPAPPPEHDPAFAAVVEVVQSTYAYMPAALAGY
                     LAGVGVVTMLYWPIASAALLAPWIGAFVAMCAVRAVVIQRFRRAAPHTRDEWLRWRQR
                     SSVGTVVAAALWGATGWIFYGLGNEPQQATGLIIVIYTFTVAGIPVLSTQPRLYLAYA
                     SLCLLPLVARIALVGDVYSYQLAGELVLIISLTTVLANSYRQALQRAIDLKVQADELA
                     AQLRVETLAAEGARREAEIANRAKTQFFTAASHDLRQPLHAMGLFAEALRQRVREPAG
                     PPQGRTPEGEARRVNNDLEVAQLVNSINESVDALEGLFSELLDITRIDSGVIEVHPQH
                     FEVGDILRKLRLHFEPGAFEKGLALRLRGGGRVVFADPLLVERILRNLVSNAIRYTND
                     GSVLVGCRQHGDRVRLQVWD"
     gene            complement(1238..1966)
                     /locus_tag="VNU48_04360"
     CDS             complement(1238..1966)
                     /locus_tag="VNU48_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI10539.1"
                     /translation="MKVLLIDDHPLILSALQSVIEGLGSDTTVTGANSARAARATLKA
                     DSDFDLVLLDLHLGDADGFEVLVELRAAYPALPVVVLSASDRSSDVIRAIDAGAMGFV
                     PKRASNETLFEALHMVMSGGIYVPPMTMGSEPAAKPEGDTVPSVLRVVREQAQDSDFP
                     TNTVPLADLGLTPRQTDVLALLLKGQPNKLIARELNVSVETVKDHVAAVLRTLGVSSR
                     TQAVLAVSQMSGQRHLPAWRNSGR"
     gene            complement(2038..2490)
                     /locus_tag="VNU48_04365"
     CDS             complement(2038..2490)
                     /locus_tag="VNU48_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWI10540.1"
                     /translation="MRSFEHLSDLQALVGQTIGVSDWITVTQQRIQLFADATGDHQWI
                     HLDVERAAKGPYGATIAHGFLTLSLLPEMAASAFEVLDTRMGVNYGLGKVRFPAPVPS
                     GSRLRGHFKLTRYEPLEGGAQLTVEVTMEREGSDKPVCVAESLSRRYT"
     gene            complement(2525..3142)
                     /locus_tag="VNU48_04370"
     CDS             complement(2525..3142)
                     /locus_tag="VNU48_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWI10541.1"
                     /translation="MPVIVVANPKGGVGKTTLATNIAGYFAARGHKVMLGDVDRQQSA
                     RTWLALRPAGLAPIAAWEVAHDEVVRPPKGTTHVVLDTPAGLHGKRLDEVMKLADKVL
                     IPLQPSIFDIHATHEFVRQLLAHKRSERVQFAIVGMRTREGTISTDQLRGFLDGLKLP
                     LLGFLRDTQNYVHLAAHGLTLWDVGSVRLGRDIEQWQPLAQWLDA"
     gene            3259..4005
                     /locus_tag="VNU48_04375"
     CDS             3259..4005
                     /locus_tag="VNU48_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWI10542.1"
                     /translation="MAESPIAVIDTAAAPSPGPDVVVITAHPQMEHSRINRRLMRAAR
                     RLPASADSPLIVVRDLYALYPDYLIDTAQEQALLVSAKLIVWQHPIHWYSMPPLMKLW
                     LDDVLAFGWAYGPGGTALRGKDLWLVATTGGPEESYHPNSYNRYFFEDFLPPYEQTAN
                     LCGLRFLPPLLLHGAHRADEAAVEAHLEVYAQRLASYPRWPELDELLYGLPAQVPEDA
                     RPGDDADDASDPPLPLSAPNSGASAARKAA"
BASE COUNT          587 a         1435 c         1425 g          616 t
ORIGIN      
        1 gtcccagacc tgcagccgca cccgatcgcc gtgctggcgg cagcccacca gcacgctgcc
       61 gtcgttggtg tagcggatcg cgttcgacac caggttgcgc aggatgcgct cgaccagcag
      121 cgggtcggcg aacacgaccc gcccgccgcc gcgcaggcgc agcgccaggc ccttctcgaa
      181 cgcgcccggc tcgaagtgca ggcgcagctt gcgcagaatg tcgccgacct cgaagtgctg
      241 cggatggacc tcgatcacgc cgctgtcgat gcgggtgatg tcgagcagct cggaaaacag
      301 gccttcgagc gcgtcgaccg attcgttgat gctgttgacg agctgcgcga cctcgaggtc
      361 attgttgacc cttcgagctt cgccctccgg ggttcgccct tggggcggcc cggcgggctc
      421 acgcacacgc tggcgcaagg cctccgcgaa caggcccatc gcgtgcagcg gctggcgcag
      481 gtcgtggctc gcggcggtga agaactgcgt cttcgcgcgg ttcgcgatct cggcctcgcg
      541 gcgcgcgcct tcggccgcca gcgtctcgac gcgcaactgc gcggcgagct cgtcggcctg
      601 caccttcagg tcgatcgcgc gctgcagggc ctggcggtag ctgttcgcca gcaccgtggt
      661 caggctgatg atcaggacca gctcgccggc gagctggtag ctgtagacgt cgcccaccag
      721 cgcgatgcgt gccaccagcg gcagcaggca caggcttgcg taggcgaggt acaggcgcgg
      781 ctgcgtcgac agcaccggga tgccggccac cgtgaaggtg tagatcacga tgatcaggcc
      841 ggtggcctgc tgcggctcgt tgcccagccc gtagaagatc cagccggtcg cgccccacag
      901 cgccgcggcc accacggtgc cgacactcga gcgctggcgc cagcgcagcc actcgtcgcg
      961 cgtgtgcggc gcggcgcggc gaaagcgctg gatcacgacc gcacgcaccg cacacatcgc
     1021 gacgaacgcg ccgatccacg gcgcgagcaa cgcggccgac gcgatcggcc agtacagcat
     1081 cgtgaccacg ccgacgccgg cgaggtagcc ggccagcgcg gccggcatgt aggcgtaggt
     1141 gctctggacg acctcgacga cggcggcgaa ggccgggtcg tgttcaggcg gcggcgcggg
     1201 ggtctggtcg gcagcggccg ccgcgatggc gctcaactca gcgtccggag ttgcgccacg
     1261 ccggcaggtg gcgctgcccc gacatctggc tcaccgccag cacggcctgg gtgcgcgagc
     1321 tgacgcccag cgtgcgcagc accgccgcga cgtggtcctt gacggtctcg accgagacgt
     1381 tcagctcgcg cgcgatcagc ttgttcggct ggcccttcag cagcagcgcg agcacgtcgg
     1441 tctggcgcgg cgtcaggccg agatcggcca gcggcacggt gttcgtgggg aagtcgctgt
     1501 cctgcgcctg ctcgcgcacc acgcgcagca cgctgggcac ggtgtcgccc tcgggcttgg
     1561 cggcgggctc ggagcccatc gtcatcggcg gcacgtagat gccgccggac atcaccatgt
     1621 gcagcgcctc gaacagggtc tcgttgctgg cgcgcttggg cacgaagccc atcgcgccgg
     1681 catcgatcgc gcggatcacg tcgctgctgc ggtcggaggc cgacagcacg acgaccggca
     1741 gcgccgggta ggcggcgcgc aattcgacca gcacctcgaa gccgtcggcg tcgcccaggt
     1801 gcaggtcgag cagcacgagg tcgaagtcgg agtcggcctt cagcgtcgcg cgcgcggccc
     1861 gcgcgctgtt cgcgccggtc acggtggtgt cgctgcccag gccctcgatc acggattgca
     1921 acgccgacag gatcagcggg tggtcgtcga tcagcagtac cttcatgcgg ggggcctccg
     1981 gcgagagcgc atcgtaaacc accgggtcgc ggggcgtctt ccccaagggc ggggagttca
     2041 ggtgtagcgg cgcgacagcg actcggccac gcagaccggc ttgtccgaac cctcgcgctc
     2101 catcgtgacc tcgacggtga gctgcgcgcc gccctcgagc ggctcgtacc gggtcagctt
     2161 gaagtgcccg cgcagccggc tgcccgacgg caccggcgcc gggaagcgta ccttgcccag
     2221 gccgtagttc acgcccatgc gggtgtcgag cacctcgaac gccgacgcgg ccatctcggg
     2281 cagcagcgac agggtcagga agccgtgcgc gatcgtggcg ccgtacggcc ccttggcagc
     2341 gcgctcgacg tcgaggtgga tccactgatg gtcgccggtc gcatcggcga acagctggat
     2401 gcgctgctgc gtgacggtga tccagtcgct gacgccgatg gtctggccga ccagcgcctg
     2461 caggtcgctc aggtgctcga aacttcgcat gggtgtcctt tcgtcgatga ggtggtgaag
     2521 cggttcaggc gtccagccac tgcgcgagcg gctgccattg ctcgatgtcg cgcccgagac
     2581 ggaccgagcc cacgtcccag agcgtcaggc cgtgcgcggc gaggtgcacg tagttctgcg
     2641 tgtcgcgcag gaagcccagc agcggcagct tcaggccgtc gaggaagccg cgcagttggt
     2701 cggtcgagat cgtgccctcg cgggtgcgca tgccgacgat cgcgaattgc acccgttcgc
     2761 tgcgcttgtg cgcgagcagc tggcgcacga attcgtgggt cgcgtggatg tcgaagatgc
     2821 tgggctgcag cgggatcagc accttgtcgg cgagcttcat cacctcgtcc aggcgcttgc
     2881 cgtgcaggcc ggccggcgta tcgagcacga cgtgcgtcgt gcccttgggc gggcgcacga
     2941 cctcgtcgtg cgcgacttcc caggccgcga tcggcgccaa gcccgccggc cgcagcgcga
     3001 gccaggtgcg ggccgattgc tggcggtcca cgtcgccgag catcaccttg tggccgcgcg
     3061 ccgcgaagta gccggcgatg ttggtcgcga gcgtggtctt gcccacgccg cccttcggat
     3121 tggccaccac gatcaccggc atgcaacgcc cccgtcctgt tctgatccgc tttcaggtta
     3181 tgttagcggg gctttcgaac gtgtcgccat cgagactaac cagcgacctg ccgcttcaac
     3241 cttgcccgtg cgaacctcat ggccgaatcc ccgatcgccg tcatcgacac cgctgccgcg
     3301 ccctcgcccg gccccgacgt ggtcgtcatc accgcgcacc cgcagatgga gcactcgcgc
     3361 atcaaccgcc ggctgatgcg cgcggcacgc cggctgccgg cgtcggccga ttcgccgctg
     3421 atcgtggtgc gcgacctgta cgcgctgtac cccgattacc tgatcgacac ggcgcaggag
     3481 caggcgctgc tcgtgtcggc gaagctgatc gtctggcagc acccgatcca ctggtacagc
     3541 atgccgccgc tgatgaagct gtggctcgac gacgtgctcg cgttcggctg ggcctacggg
     3601 cccggcggca ccgcgctgcg cggcaaggac ctgtggctgg tcgcgaccac cggcggcccc
     3661 gaggagtcgt accacccgaa cagctacaac cgctatttct tcgaagactt tctgccgccc
     3721 tacgagcaga ccgccaacct gtgcgggctg cgcttcctgc cgccgctgct gctgcacggc
     3781 gcgcaccgcg ccgacgaggc cgccgtcgag gcgcatctcg aggtctatgc gcagcgcctg
     3841 gccagctacc cgcgctggcc cgagctcgac gagctgctct acggcctgcc cgcgcaggtg
     3901 cccgaggacg cgcggcccgg cgacgacgcg gacgatgcgt ccgacccacc cttgccgctg
     3961 tcggccccga acagcggcgc gagtgccgcg cgcaaggcgg cgtgatggaa cacggctggc
     4021 tcaccggcac gctggtctac ctgggcgccg cggtgctcgc ggt
//
LOCUS       DATMJB010000194         2182 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_627068, whole genome shotgun sequence.
ACCESSION   DATMJB010000194 DATMJB010000000
VERSION     DATMJB010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627068"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VNU48_04380"
     CDS             <1..463
                     /locus_tag="VNU48_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI10543.1"
                     /translation="VFLVSHDRRFLDNVVTSTIVWEGDESPGRWREYEGGYEDWVVQR
                     DRARALREQAARAAGKAREAPAAKPAAAAAPVSPTKPRKLSYKEQRELDELPRRIEAL
                     EGEQKALGEFLARPESYTKEADKAMQAQVRVAAIDDELLAAMERWEQLGAR"
     gene            complement(485..1237)
                     /locus_tag="VNU48_04385"
     CDS             complement(485..1237)
                     /locus_tag="VNU48_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10544.1"
                     /translation="MKGELFDSTIPFGEPELLKASAYRRYLDELEADTVSGRTSAHLS
                     QLSPSLQADLWRHEQRGGGYEIVEAIAACIRHSSRVTIHVQCAERVVPLTVFPQERLV
                     HCPMDLNELIERHLSQLHVMHVEPAVLRPPGDAARALVGEARLYHPLTPLLWALAMRG
                     PRRELLPEIAGPAVYRVSPALDLATLPISGTLRAAIHRLHRQAISLSGLASWPAFDRE
                     RAVRLLNALYLQAGLIVSRSHPDAVRESWFGI"
     gene            complement(1283..>2182)
                     /locus_tag="VNU48_04390"
     CDS             complement(1283..>2182)
                     /locus_tag="VNU48_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HWI10545.1"
                     /translation="VGATNALDIVTRTLLRAGDSVLVDEPGWSVEYARLAALGLRVLP
                     VPRGEDGPDLAEMQRLIEAQKPAQRPRLYVTVSVLSNPTGASLSLQAAHRVLQLAQAH
                     DLHVVEDDTYAHLAPAHLPRMAALDALERTIYISGFSKILVPNWRVGFIAAPRSLVER
                     LVDTKLLTTLTTPGLTELALAHCLEQGLLRRHAERVMDRLAQARERTVKLAEAHGGRF
                     AAPPRGLFGWVDVGVDTERLAQLMLDEWLIAPGALFHATHRPTTLMRINFATSQDARF
                     WRALDRARASLRGAAAERRTGIT"
BASE COUNT          333 a          757 c          801 g          291 t
ORIGIN      
        1 ggtctttctc gtcagccacg accggcgctt tctcgacaat gtcgtgacca gcacgatcgt
       61 ctgggagggc gacgaatcgc cgggccgctg gcgcgagtac gaaggcggct acgaggactg
      121 ggtggtgcag cgcgaccgcg cccgcgcgct gcgcgagcag gccgcgcgtg cggcgggcaa
      181 ggccagggaa gcgccggcag cgaagccggc cgcggcggcg gcgccggttt ctccgaccaa
      241 gccgcgcaag ctcagctaca aggagcagcg cgaactcgac gaactgccca ggcgcatcga
      301 ggccctcgag ggcgagcaga aggcactcgg cgagttcctc gcccggcccg agtcgtacac
      361 gaaggaagcc gacaaggcga tgcaggcgca ggtccgggtc gcggcgatcg acgacgagct
      421 gctcgccgcg atggagcgct gggaacagct cggcgcgcgc tgaccgtggc gccggcgccg
      481 tggatcagat gccgaaccag ctttcccgca ccgcgtccgg gtgcgagcgg ctgacgatca
      541 gcccggcctg caggtacagc gcgttcagca accgcaccgc gcgctcgcgg tcgaaggctg
      601 gccaggacgc caggccgctc agcgagatcg cctgacggtg cagccggtgg atcgcggcgc
      661 gcagcgtgcc gctgatcggc agtgtcgcca gatcgagcgc cggcgacacc cgatagaccg
      721 ccgggccggc gatctcgggc agcagctcgc ggcgcgggcc gcgcatcgcc agcgcccaca
      781 gcaagggtgt cagcgggtgg tacagccgcg cctcgccgac cagcgcgcgc gccgcgtcgc
      841 cgggcgggcg cagaacggcc ggctcgacgt gcatcacatg caattgcgac aggtggcgct
      901 cgatcagctc gttcaggtcc atcgggcagt gcaccaggcg ctcttgcggg aagacggtca
      961 gcggcacgac gcgttccgcg cattgcacgt gaatcgtcac ccgcgacgaa tgccggatgc
     1021 acgccgcgat cgcctcgacg atctcgtagc cgccgccgcg ctgctcgtgg cgccacagat
     1081 cggcctgcag cgaggggctg agctgcgaca ggtgcgcgct cgtgcggccc gacacggtgt
     1141 cggcctcgag ttcgtcgagg taacgccggt acgcgctcgc cttcagcagc tcgggctcgc
     1201 cgaaggggat ggtggagtcg aacagctcgc ctttcatgga acgcccattt tcgcctgtaa
     1261 ctcgcgctta ccacaccccg ggtcaggtta tccctgttcg ccgttcggcc gcggcgcccc
     1321 gcaggctggc gcgcgcacga tcgagcgcgc gccagaagcg ggcgtcctgc gaggtcgcga
     1381 agttgatgcg catcagcgtg gtcggccggt gggtggcatg gaacagcgca cccggggcga
     1441 tcaaccactc gtcgagcatc agctgcgcga ggcgctcggt gtcgacaccc acgtcgaccc
     1501 agccgaacag cccgcgcggc ggcgccgcga agcgcccgcc gtgggcctcg gcgagcttca
     1561 cggtgcgttc gcgcgcctgc gcgaggcggt ccatcacgcg ctcggcgtgg cgtcgcagca
     1621 agccctgttc cagacagtgc gccaaggcca gctcggtcag gccgggcgtg gtcagcgtgg
     1681 tcagcagctt ggtgtcgacc agccgttcga ccagcgagcg cggcgccgcg atgaagccga
     1741 cgcgccagtt cggcaccagg atcttcgaga aacccgagat gtagatcgtg cgttccagcg
     1801 cgtccagcgc ggccatgcgc ggcaggtgcg ccggtgcgag gtgggcgtag gtgtcgtcct
     1861 cgacgacgtg caggtcgtgc gcctgggcca gctgcagcac gcggtgcgcg gcctgcagcg
     1921 acagcgaggc gccggtcggg ttgctcagca ccgagacggt cacgtacagg cgcggtcgct
     1981 gcgccggttt ctgggcctcg atcaggcgct gcatctcggc gagatcgggc ccatcttcgc
     2041 cgcgcggcac cggcagcacg cgcaggccga gtgcggccag gcgcgcgtac tcgaccgacc
     2101 agcccggctc gtcgaccagc acgctgtcgc cggcacgcag cagcgtgcgc gtgacgatgt
     2161 cgagtgcgtt cgtcgcgccg ac
//
LOCUS       DATMJB010000195         3931 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_845032, whole genome shotgun sequence.
ACCESSION   DATMJB010000195 DATMJB010000000
VERSION     DATMJB010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845032"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1554
                     /locus_tag="VNU48_04395"
     CDS             <1..1554
                     /locus_tag="VNU48_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HWI10546.1"
                     /translation="QSSCNKDFSCVKGFCPSFLTITPKPEPAADGSGKRKKKGRIPVF
                     ERALPEPLIKVNASNGFGIHLMGIGGTGSVTVAAMIAHAARLEGKHVVGLDQTGLAQK
                     GGAVVSDLKISNQVADGANKISDGGTDLYLGFDILTATDPKNLDKCLAERTIAVVSTT
                     RTPTGQMVSNRKVLFPIVPDLRAGIDRVTRQADNVYIDAQAMAEGLFGDAMAANSFMV
                     GVAFQAGGMALQAASIEAAIGHAGVGVEQSLAAFRWGRMAVVDRAFVEAEIARHAPKT
                     ATPELSAAARAIVEAVGAQGETRRLLELRVPDLIDYQDAAYARRYADVVRRVVAAEQR
                     AVPGASALAEAAARYLYKLMAYKDEYEVARLHTDPAFLAQLDAQFKHGYTLKYHLAPP
                     AFAKRDPATGHLVKRQFGPWMQGALRLLAKFKGLRGGALDVFGRTEERRRDRQRIADY
                     IKELDDICAQLVPANHAAAVALASVPDEIRGYGHVREDSAAEAGSLREQRLQAFRSPQ
                     GVGVRLVVS"
     gene            1642..2139
                     /locus_tag="VNU48_04400"
     CDS             1642..2139
                     /locus_tag="VNU48_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10547.1"
                     /translation="MGQHRVAQQPIGAAERPPLRRARDAQAALESVVAVQVGTAEDPS
                     AQIAVASTLETLDYLKLALFRSFGHMVGSAAAGAPAPLHDRLLYRYQSARVADLCAEQ
                     VSKLFQACGAGGVYLNRPLVRRFLDIHTARSHVANHAARAGTQPGRLLHGPGQPRPHG
                     LRHAP"
     gene            complement(2283..3029)
                     /locus_tag="VNU48_04405"
     CDS             complement(2283..3029)
                     /locus_tag="VNU48_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI10548.1"
                     /translation="MALRDKVVLVTGAAQGIGQATALALARAGARVVVSDRDDRHAAE
                     QLAALERCGAEAHFVRADVMHSADCTALVDAALARFGRLDCAVNNAGVSEGPPPRPLA
                     DYPDALWEHIVGVNLGGVFRCMREELRAMRDGGHGGAIVNIASVAGQIAFAGVAGYVA
                     SKHGVVGLTKAAAVDYGALGIRCNAIGPGFIDTRMLPPQAHDWMRSVTPAGRLGSADE
                     IAQAAVWLCSDAASFVNGAYLAADGGFLAQ"
     gene            complement(3029..>3931)
                     /locus_tag="VNU48_04410"
     CDS             complement(3029..>3931)
                     /locus_tag="VNU48_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI10549.1"
                     /translation="VLDAINTAWPAIDWRTIGVEEARALLGGPSMFAPGDEVASVTDR
                     SISGPGGSLRLRLYQPPGTAGPLPMTVYLHGGGFCTGAPEGHDNVCRSLARRASTLVV
                     SVDYRLAPESPFPAAVHDALAALRWVREHAAEIGGDAARVAVAGDSAGATLATVIARL
                     ARDEGPALRHQLLLYPVTDSGCDFASYTELAHGHLLTTEMMRWYWRHYLSRPQDRLDP
                     QACPLRQSDLAAVAPATFVIAGFDPASDEGRAYAAALRDAGVAVSVHEWPDQIHGFAS
                     MLGAIDAADAALDQGADALRRAFA"
BASE COUNT          592 a         1420 c         1397 g          522 t
ORIGIN      
        1 cagagctctt gcaacaagga cttctcctgc gtcaagggct tctgcccgtc gttcctgacg
       61 atcacgccca agcccgagcc cgcggccgac gggagcggca agagaaagaa gaagggccgc
      121 atcccggtgt tcgaacgtgc gttgcccgag ccactgatca aggtgaatgc cagcaacggc
      181 ttcggcatcc acctgatggg tatcggcggc accggctcgg tcaccgtcgc ggccatgatc
      241 gcccacgcgg cgcggctcga aggcaagcat gtcgtcggtc tggaccagac cggcctggcg
      301 caaaagggcg gtgcggtggt ctccgacctg aagatcagca accaggttgc cgacggtgcg
      361 aacaagatct ccgacggcgg caccgacctg tacctgggct tcgacatcct caccgcgacc
      421 gatccgaaga acctcgacaa gtgcctggcc gaacgcacca tcgcggtggt gtcgacgacg
      481 cgcacgccca ccgggcagat ggtgtccaac cgcaaggtgc tgttcccgat cgtgcccgac
      541 ctgcgtgccg gcatcgaccg cgtcacgcgc caggccgaca acgtctacat cgacgcccag
      601 gccatggccg agggcctgtt cggcgacgcg atggcggcca acagcttcat ggtcggcgtg
      661 gccttccagg ccggcggcat ggccttgcag gcggcgtcga tcgaagccgc gatcggccat
      721 gccggcgtcg gtgtcgagca gagcctggcg gcgttccgct ggggccgcat ggcggtggtc
      781 gaccgcgcct tcgtcgaggc cgagatcgcc cgccacgcgc cgaagacggc cacgcccgag
      841 ctgtcggccg ccgcacgcgc catcgtcgaa gcggtcggcg cgcagggcga gacgcggcgc
      901 ctgctcgagc tgcgcgtgcc cgacctgatc gactaccagg acgcggccta cgcgcggcgt
      961 tatgccgacg tggtgcggcg cgtcgtcgcc gccgagcagc gcgccgtgcc gggcgcgagc
     1021 gcgctggccg aggccgcggc gcgctacctg tacaagctga tggcctacaa ggacgaatac
     1081 gaggtcgcgc ggctgcacac cgacccggcc ttcctggccc agctcgacgc gcagttcaag
     1141 cacggctaca cgctcaagta ccacctcgca ccgccggcgt tcgccaagcg cgacccggcc
     1201 acgggccacc tggtcaagcg gcagttcggg ccgtggatgc aaggcgccct gcggctgctg
     1261 gcgaagttca agggtctgcg cggcggcgcg ctcgacgtct tcggcaggac cgaggagcgc
     1321 cgtcgcgacc gacagaggat cgccgactac atcaaggagc tcgacgacat ctgcgcgcag
     1381 ctggtcccgg ccaaccacgc cgcggcagtg gcactggcca gcgtgcccga cgagatccgc
     1441 ggctacggcc acgtgcgcga ggacagcgcc gccgaggccg gcagcttgcg cgagcagcgg
     1501 ctgcaggcgt ttcgcagccc gcagggggtg ggggtcaggc tcgtcgtgtc gtgatctgcc
     1561 cgggccggcg gccgccttca gatcatcgag acgccatgac tcaagatcag ccgcagcagg
     1621 ctgctttgcc ggctcacccc cgtggggcag caccgcgttg cacagcagcc gatcggcgcg
     1681 gctgaacggc cgcccctccg gcgggcgcga gatgcgcagg cggcactcga gtccgtcgtc
     1741 gctgtgcagg tcggcaccgc ggaggatccc agcgcccaga tcgccgtggc gagcacgctg
     1801 gagaccctcg actacctgaa gctcgcgctg tttcgcagct tcgggcacat ggtcggcagt
     1861 gccgccgccg gcgcgccggc gccgctgcat gaccgcctgc tctatcgcta ccagtcggcg
     1921 cgtgtggccg acctgtgtgc cgagcaggtc agcaagctgt tccaggcctg cggggccggc
     1981 ggtgtgtacc tgaaccggcc gctggtgcgg cgctttctcg acatccatac ggcgcgctcg
     2041 cacgtggcca accatgccgc tcgcgctggg acgcaacctg ggcggctgct gcatgggcct
     2101 ggacaaccgc gaccccacgg tctgaggcac gcgccgtgat cgtcgctgtc gatctgcaac
     2161 acgaggcgtg cctgctcgaa gcggacaact tccgtgcctt caagccgtgc acgccgcagg
     2221 cgcaggccga cgtcgcggcg ctgcaggcgc gtcacggcgg catgctgcgg ctgggcttcg
     2281 gctcactgcg ccaggaagcc gccgtcggcg gccaggtagg ccccgttgac gaacgacgcc
     2341 gcgtcgctgc acagccacac ggcggcctgg gcgatctcgt cggcgctgcc cagccggccg
     2401 gccggcgtca ccgagcgcat ccagtcgtgg gcctgcggcg gcagcatgcg ggtgtcgatg
     2461 aaaccggggc cgatggcgtt gcagcggatc cccagcgcgc catagtccac ggccgccgcc
     2521 ttggtcaggc cgaccacgcc gtgtttgctg gccacgtagc cggccacgcc ggcaaaggcg
     2581 atctgcccgg ccaccgaggc gatgttgacg atggcgccgc cgtggccgcc atcgcgcatc
     2641 gcacgcagtt cctcgcgcat gcagcggaag acgccgccga ggttgacgcc gacgatgtgt
     2701 tcccacagcg cgtcggggta gtcggccagc ggccgcggcg gcgggccttc ggagacgccg
     2761 gcgttgttga cggcgcagtc gagccggccg aagcgtgcca gggcagcgtc gaccagcgcg
     2821 gtgcagtcgg cgctgtgcat cacgtcggcc cgcacgaaat gcgcctcggc gccgcagcgc
     2881 tcgagcgccg ccagctgctc ggccgcatgg cggtcgtcgc gatcggacac caccacgcgc
     2941 gcgcccgcac gtgccagcgc cagcgccgtg gcctggccga tgccctgcgc cgcgccggtg
     3001 accagcacca ccttgtcgcg cagcgccatc aggcgaaggc gcgacgcagc gcgtcggcac
     3061 cctggtcgag cgcggcgtcg gcggcgtcga tcgcaccgag catgctggcg aagccgtgga
     3121 tctggtcggg ccattcgtgc acgctcacgg ccaccccggc gtcgcgcagc gccgccgcat
     3181 aggcacgccc ctcgtcgctg gccggatcga aacccgcgat cacgaaggtc gccggcgcca
     3241 ccgcggccag gtcgctctgg cgcagcgggc aggcctgggg gtccagccga tcctgcgggc
     3301 gcgacaggta gtgccgccag taccagcgca tcatctcggt ggtcagcagg tggccgtgcg
     3361 cgagttcggt gtaggaggca aagtcgcagc cgctgtcggt cacgggatag agcagcaact
     3421 ggtggcgcaa cgccggcccc tcgtcgcgcg ccaggcgggc gatgaccgtg gccagcgtgg
     3481 cgccggcgct gtcgccggcc acggccacgc gcgcggcatc gccgccgatc tcggccgcat
     3541 gctcgcgcac ccagcgcagc gccgccaggg cgtcgtgcac cgccgccggg aacggtgact
     3601 cgggcgcgag ccggtagtcg accgatacca ccagcgtcga ggcacgccgg gccaaggagc
     3661 ggcagacgtt gtcgtgccct tcgggcgcgc cggtacagaa gccgccgcca tggagataca
     3721 cggtcatcgg cagcgggcct gccgtaccgg gtggctggta cagccgcagg cgcagcgagc
     3781 cacccgggcc cgaaatgctc cggtcggtga ctgaggcgac ctcgtccccg ggcgcgaaca
     3841 tcgatggacc gcccaacagc gcgcgcgcct cctccacgcc gatcgttcgc caatcgatcg
     3901 ccggccaggc ggtgttgatc gcgtcgagca c
//
LOCUS       DATMJB010000196         3149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_220510, whole genome shotgun sequence.
ACCESSION   DATMJB010000196 DATMJB010000000
VERSION     DATMJB010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220510"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VNU48_04415"
     CDS             <1..560
                     /locus_tag="VNU48_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWI10550.1"
                     /translation="YQGFGDGIEADAYAARALASAGVSFFIANSFSKSMSVYGERCGA
                     LSVVCPNAHEAALVLGQMKFTVRRNYSSPPIHGGQIVATVLTEPELRRDWERELGAMR
                     ERILAMRRQLHGVLSARLPGRNFDYFLTQRGMFSYTGLTAAQVDALRETHAVYMVRSG
                     RICVAGLNSGNVEATAMAMAAVLGG"
     gene            complement(600..1886)
                     /locus_tag="VNU48_04420"
     CDS             complement(600..1886)
                     /locus_tag="VNU48_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HWI10551.1"
                     /translation="MFCAIDFGTSNSAIAIPDAAGAMRLVELEPGQRTMPTAVFYFVE
                     GPDLDGPPRAFGRAAIAAYVDGLDGRLMRSMKSILGSNLIDQTTDVGGGRGAKYLDIV
                     AGYLKHLKACAQAQAGAPIRRAVLGRPVFFVDDDPVRDAQAQAALEAAARQVGFEELQ
                     FQYEPIAAAFDYERSAVREQIVLVADIGGGTSDFSVVRVGPQRINRPDRKDDILANHG
                     VHVAGTDFDRRVELTAILPAYGFGAMGPSVAGAPAREVPSGVYFDLATWHLINTVYSP
                     ARVAELRGMRGFYADARQHERLMVVVDERLGHELMARAEQAKIDVAGGGATTIDLSHT
                     ERGLCAALSEQAAVQAMAADIERIVGAARAAVAQAGLVPAQIDALYFTGGSTGLRLLA
                     DRIGAVFPTAQVVRGDRFASVVTGLGLHAQRRFAPG"
     gene            complement(2087..>3149)
                     /locus_tag="VNU48_04425"
     CDS             complement(2087..>3149)
                     /locus_tag="VNU48_04425"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWI10552.1"
                     /translation="PARADGVLQLVVREPVGPVAAFTPWNFPINQAVRKVSAALAAGC
                     SVILKGPEETPASVAALVQAYADAGLPAGVLNLVFGVPSEISEYLIPSPVIRKITFTG
                     STPVGKRLAALAGQHMKRITMELGGHAPAIVFGDADVDAAVKQLAGSKFRNAGQVCIA
                     PTRFLVHESVYARFVDGFVKAAGSLKVGDGLDPANRMGPLANSRRVEAMQMFVDDAVA
                     KGAKLLTGGKRIGSTGNFFEPTVLGHVPASARIMSEEPFGPIAPIVSFSSFDEVVAEA
                     NRLPFGLASYAYTRSAKTVNAIGAAIEAGMVTINHVGFALPELPFGGVKDSGYGSEGG
                     SEAIEAYLNSKLITQLGVE"
BASE COUNT          461 a         1116 c         1131 g          441 t
ORIGIN      
        1 cgtaccaggg cttcggcgac ggcatcgagg ccgacgccta tgcggcgcgc gcgctggcca
       61 gcgcgggcgt gagcttcttc attgcgaact cgttctcgaa gagcatgagc gtgtacggcg
      121 aacgctgcgg cgcgctcagc gtggtctgcc cgaatgcgca cgaagccgcg ctggtgctgg
      181 gccagatgaa gttcacggtg cggcgcaatt actcgagccc gccgatccac ggcggccaga
      241 tcgtcgcgac ggtgctgacc gaacccgaac tgcgccgcga ctgggagcgc gagctcggcg
      301 cgatgcgcga gcgcatcctg gcgatgcgcc gccagctgca cggcgtgctc agcgccaggc
      361 tgccggggcg caacttcgac tacttcctga cccagcgcgg catgttcagc tacaccggcc
      421 tcacggccgc gcaggtcgat gcgctgcgcg agacgcacgc cgtctacatg gtgcgctcgg
      481 ggcgcatctg cgtggcgggg ctgaacagcg gcaacgtcga ggccaccgcg atggcgatgg
      541 cggcggtgct gggcggctga cggggcgtcg cgcctgccgg tccgcccggt gaatggtcgt
      601 caaccaggcg cgaaccggcg ctgcgcgtgc agcccgaggc ccgtcacgac gctggcaaag
      661 cggtcgccgc gcaccacctg ggccgtcggg aacaccgcgc cgatgcggtc ggcgaggagc
      721 cgcaggccgg tcgagccgcc ggtgaagtag agcgcgtcga tctgcgcggg cacgagcccg
      781 gcctgcgcca ccgctgcgcg cgccgcgccg acgatgcgct cgatgtcggc ggccatggcc
      841 tgcaccgcgg cctgctcgct cagcgcggcg cacaggccgc gctcggtgtg gctcaggtcg
      901 atggtggtcg cgccgccgcc ggcgacatcg atcttggcct gctcggcgcg cgccatcagc
      961 tcgtggccga ggcgctcgtc gacgaccacc atcaggcgtt cgtgctgcct ggcgtcggca
     1021 tagaagccgc gcatgccgcg cagctcggcg acgcgcgccg ggctgtagac ggtgttgatc
     1081 aaatgccagg tcgcaaggtc gaagtacacg ccgctgggca cctcgcgcgc gggcgcgccc
     1141 gccacgctcg gccccatcgc gccgaagccg taggcgggca ggatcgccgt cagctcgacg
     1201 cgccggtcga agtcggtgcc ggccacgtgc acgccgtggt tcgcgaggat gtcgtccttg
     1261 cggtccggcc ggttgatgcg ctgcgggccg acccgcacga ccgagaagtc cgaggtgccg
     1321 ccgccgatgt cggccaccag cacgatctgc tcgcgcacgg cgctgcgctc gtagtcgaac
     1381 gcggcggcga tcggctcgta ctggaactgc agctcctcga agccgacctg gcgcgccgcc
     1441 gcctcgagtg cggcctgcgc ctgcgcgtcg cgcacggggt cgtcgtcgac gaagaacacc
     1501 gggcggccga gcaccgcgcg gcggatcggc gcgccggcct gcgcctgcgc gcatgccttg
     1561 aggtgcttca ggtagccggc gacgatgtcg aggtacttcg cgccgcgacc gccgccgacg
     1621 tcggtggtct ggtcgatcag gttgctgccc aggatgctct tcatcgagcg catcaggcgg
     1681 ccgtccagcc cgtcgacata ggccgcgatc gccgcgcggc cgaatgcgcg cggcgggccg
     1741 tcgagatcgg ggccctcgac aaagtagaag acggcggtcg gcatcgtccg ctgcccgggc
     1801 tcgagctcga ccagccgcat cgcgccggcg gcgtcgggaa tggcgatcgc ggaattggag
     1861 gtgccgaagt cgatggcgca gaacatggga agggaaggcc ggcaccggca gcaggacggg
     1921 gggcgcgatt ctactgagct tcgtccggat ccagcagaag aactggggcg ccgcgatcga
     1981 cgagctcagc gcatcaacga caccgcagcg ccgactggaa caaccggatg cgctacagcc
     2041 tgcgcaagcg cccgacgccc gaccacgcgg cggccgagaa gttctgttac tccacgccca
     2101 gctgcgtgat gagcttgctg ttcagatagg cctcgatcgc ctcgctgccg ccctcggagc
     2161 cgtagcccga atccttgacg ccgccgaacg gcagttcggg cagcgcgaag ccgacgtggt
     2221 tgatcgtgac catgccggcc tcgatcgccg caccgatcgc gttcaccgtc ttggccgagc
     2281 gcgtgtaggc gtacgaggcc aggccgaacg gcaggcggtt cgcctcggcg acgacctcgt
     2341 cgaagctgct gaacgacacg atcggcgcga tcgggccgaa cggttcctcg ctcatgatgc
     2401 gcgcgctcgc cggcacgtgg ccgagcacgg tcggctcgaa gaagttgccg gtgctgccga
     2461 tgcgcttgcc gcccgtcagc agcttcgcgc ccttcgcgac cgcgtcgtcg acgaacatct
     2521 gcatcgcctc gacgcgccgg ctgttcgcca gcggccccat ccggttcgcc gggtccagcc
     2581 cgtcgccgac cttgaggctg cccgcggcct tgacgaagcc gtcgacgaag cgcgcgtaga
     2641 ccgactcgtg caccaggaag cgcgtcggcg cgatgcagac ctgtccggcg ttgcggaact
     2701 tcgacccggc cagctgcttg acggccgcgt cgacatcggc atcgccgaac acgatcgccg
     2761 gcgcgtggcc gccgagctcc atcgtgatgc gcttcatgtg ctggccggcc agcgcggcga
     2821 gccgcttgcc gaccggcgtc gagccggtga aggtgatctt gcggatcacc gggctgggga
     2881 tcaggtactc ggagatctcg gacggcacgc cgaacaccag gttcagcacg cccgccggca
     2941 gcccggcatc ggcgtaggcc tgtaccagcg cggccacgct cgcgggcgtt tcctccggcc
     3001 ccttcaggat caccgagcag cccgcggcca gcgccgccga gaccttgcgc accgcctggt
     3061 tgatggggaa gttccagggc gtgaacgcgg ccaccgggcc gaccggttcg cgcacgacca
     3121 gctgcagcac gccgtcggcg cgcgccggc
//
LOCUS       DATMJB010000197         3366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_477667, whole genome shotgun sequence.
ACCESSION   DATMJB010000197 DATMJB010000000
VERSION     DATMJB010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3366
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477667"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VNU48_04430"
     CDS             <1..237
                     /locus_tag="VNU48_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HWI10553.1"
                     /translation="YMAFFPLFVDPAHHRGVLTFGVMAATIAALTFLYGLVVVLLTHR
                     LAERLRANPKVSGALHKLAGVFLIGFGFKLALSR"
     gene            complement(244..1542)
                     /gene="hmgA"
                     /locus_tag="VNU48_04435"
     CDS             complement(244..1542)
                     /gene="hmgA"
                     /locus_tag="VNU48_04435"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HWI10554.1"
                     /translation="MTTTPLHYQTGFGNEYASEAVPGALPQGRNSPQRAPLALYPELV
                     SGTAFTAPRRENRRSWLYRRQPSVVAGRFVAYRQALWCTGGDTRTALAPEPMRWGPFP
                     IEPSADADFVDGMRTLAANGDAEAQTGLAAHVYLATRSMAQRAFVNADGELLIVPQQG
                     RLQITTELGVLEVKPGEIALLPRGVAFKVALPDGPSRGYVCENYGAQFRLPELGPIGS
                     NGLANARDFKAPVAAFETGTGAYELVKKSGGHFWRAPLVQSPFNVVAWHGNLAPLKYD
                     TANFMTIGSISFDHPDPSIFTVLTSPSDTPGTANCDFVIFPPRWLVMEDTFRPPWYHR
                     NLMSEFMGLVLGEYDAKPEGFKPGGASLHNCMVPHGPDQEAFERASGATLAPQKLDNT
                     LAFMFESRYRFIPTEFALRSPQLDQRYAECWSGLPDRFKP"
     gene            complement(1563..1769)
                     /locus_tag="VNU48_04440"
     CDS             complement(1563..1769)
                     /locus_tag="VNU48_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2783 domain-containing protein"
                     /protein_id="HWI10555.1"
                     /translation="MTLITAPHLSAPDDFYEALIDAHRELSPAQSHALNARLVLLLAN
                     HIGTLEVLKEALAAARSSAAPPPA"
     gene            complement(1783..>3366)
                     /locus_tag="VNU48_04445"
     CDS             complement(1783..>3366)
                     /locus_tag="VNU48_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI10556.1"
                     /translation="IVVGAGPVGLSLAVDLAQRGIQVLLLDNDHKLSTGSRAICFAKR
                     TLEIFDRLGCGERMVAKGVCWNVGKVFFRDAQIYQFDLLAEPGHARPAFINLQQYCVE
                     GYLAQRAAELPLIDLRWKNKVVGLTHGADGATLTIETPDGPYRLHADHVVACDGSRST
                     LRELLGQESRGRVFRDRFLIADVRMKLDRPAERWFWFDPPFHRHQSVLLHMQPDNVWR
                     IDFQLGWDADPEEAKKPENIIPRVKALLGDYVPFELEWASVYTFACTRMARFRVGPVL
                     FAGDAAHGVSPFGARGANSGVQDADNLGWKLALVVAGCAPEALLDSYASEREYAADEN
                     ILHSTRATDFITPKSEISRLFRDAVLDLARQHPFARTLVNSGRLSVPATLHESPLNTP
                     DSARFEGRMQPGAAAADAPVLRADERASWLLRELGDGFTALVYGEADAHALARRLRVA
                     GDGLVPLKLLLVRPCTAEAHADAPDCVIDHEGLVAERYDMLPGTVVLLRPDQHVCARW
                     REPSADAVRAALARALALS"
BASE COUNT          474 a         1125 c         1249 g          518 t
ORIGIN      
        1 tacatggcgt tcttcccgct gttcgtcgac ccggcgcacc accgcggcgt gctgaccttc
       61 ggcgtgatgg ccgcgacgat cgccgcgctg accttcctct acggcctggt cgtggtgctg
      121 ctcacgcatc gccttgccga gcggttgcgc gcgaacccga aggtgtcggg cgcgctgcac
      181 aagctggcgg gcgtgttcct gatcggcttc ggcttcaagc tcgcgctgtc gcgctgagcg
      241 gcttcagggc ttgaagcggt ccggcaagcc ggaccagcat tcggcatagc gctgatcgag
      301 ctgcgggctt cgcaacgcga actcggtcgg gatgaagcga tagcggctct cgaacatgaa
      361 ggccagcgtg ttgtccagct tctgcggcgc cagggtggcg ccgctggcgc gctcgaaggc
      421 ctcttggtcc ggcccgtgcg gcaccatgca gttgtgcagg ctcgcgccgc ccggtttgaa
      481 gccctcgggc ttggcgtcgt actcgccgag caccaggccc atgaactcgg acatgaggtt
      541 gcggtggtac cagggtgggc ggaacgtgtc ctccatcacc agccagcgtg gcgggaagat
      601 cacgaagtcg cagttcgcgg tgcccggcgt gtcgctcggc gaggtcagca ccgtgaagat
      661 cgacgggtcg gggtggtcga agctgatcga gccgatcgtc atgaagttcg cggtgtcgta
      721 cttcagcggc gcaagattgc cgtgccacgc gacgacgttg aacggcgact gcaccagcgg
      781 cgcgcgccag aagtggccgc cggacttctt gacgagttcg tacgcgcccg tgccggtctc
      841 gaacgcggcc accggcgcct tgaagtcgcg cgcgttcgcc agcccgttcg aaccgatcgg
      901 cccgagttcg ggcagacgga actgcgcgcc gtagttctcg cacacgtagc cgcgcgacgg
      961 cccgtcgggc aacgccacct tgaacgcgac gccgcgcggc agcagcgcga tctcgcccgg
     1021 cttcacctcg agcacgccga gctcggtggt gatctgcagg cgcccctgct gcggcacgat
     1081 cagcagttcg ccgtcggcgt tgacgaacgc gcgctgcgcc attgagcgcg tcgcgaggta
     1141 gacgtgggcg gcgaggcctg tctgcgcctc ggcatcgccg ttggccgcca gcgtgcgcat
     1201 gccgtcgacg aagtcggcat cggcggacgg ctcgatcggg aacgggcccc agcgcatcgg
     1261 ctcgggcgcg agcgcggtgc gcgtgtcgcc gccggtgcac cacagcgcct gcctgtacgc
     1321 gacgaagcgg cccgccacca ccgagggctg gcgccggtac agccagctgc gccggttctc
     1381 gcggcgcggc gcggtgaacg cggtgcccga caccagctcg gggtacagcg ccagcggcgc
     1441 gcgctgcggg ctgttgcggc cctgcggcag cgcgccgggc acggcctcgc tcgcgtattc
     1501 gttgccgaag ccggtctggt agtgaagcgg tgtggtggtc atggatgtcc gcgaaaggtc
     1561 ggtcaggccg gcggcggtgc cgcgctgctg cgggccgcgg cgagcgcttc cttcagcacc
     1621 tcgagcgtgc cgatgtgatt ggccagcagc agcaccaggc gcgcgttcag cgcgtggctc
     1681 tgcgccggcg agagctcgcg gtgcgcgtcg atcagcgcct cgtagaagtc gtcgggtgcg
     1741 ctcaggtgcg gggcggtgat cagtgtcatc tcgggggtct cgtcaggaca gcgccaacgc
     1801 gcgcgccagc gccgcacgca ctgcgtcggc cgaaggttcg cgccagcgcg cgcagacgtg
     1861 ctggtccggg cgcagcagga ccaccgtgcc cggcagcatg tcgtagcgct cggcgaccag
     1921 cccttcgtga tcgatcacgc agtcgggggc gtcggcgtgc gcttcggcag tgcagggccg
     1981 caccagcagc agcttcaggg gcaccaggcc gtcgccggcg acgcgcagcc ggcgcgcgag
     2041 cgcatgcgca tcggcctcgc cgtagacgag cgcggtgaag ccgtcgccga gctcgcgcag
     2101 cagccagctg gcgcgctcgt ccgcgcgcag caccggcgcg tcggcggcgg cggcgccggg
     2161 ctgcatccgg ccctcgaagc gggcgctgtc gggcgtgttc agcggcgact cgtgcaaggt
     2221 cgccggcacc gacagccggc cgctgttgac cagcgtgcgg gcgaacgggt gctgccgcgc
     2281 gaggtcgagc accgcgtcgc ggaacagccg gctgatctcg ctcttcggcg tgatgaaatc
     2341 ggtggcgcgg gtggagtgca ggatgttctc gtcggcggcg tactcgcgct cgctggcgta
     2401 gctgtcgagc agcgcctcgg gcgcgcagcc ggcgacgacg agcgcgagct tccagccgag
     2461 gttgtcggcg tcctgcacgc ccgagttcgc gccgcgcgcg ccgaacggcg acacgccgtg
     2521 cgcggcgtcg ccggcgaaca gcaccggccc gacgcggaat cgcgccatgc gcgtgcaggc
     2581 gaaggtgtag acgctggccc attcgagctc gaagggcacg tagtcgccca acagcgcctt
     2641 gacgcgcggg atgatgttct cgggtttctt cgcttcttcc gggtcggcgt cccagccgag
     2701 ctggaagtcg atgcgccaga cgttgtcggg ctgcatgtgg agcagcacac tctggtggcg
     2761 atggaacggc ggatcgaacc agaaccagcg ctcggccggc cggtcgagct tcatcctcac
     2821 gtcggcgatc aggaagcggt cgcggaacac gcggccccgg ctttcctgac ccagcagttc
     2881 gcgcaaggtg gagcggctgc cgtcgcaggc gacgacgtgg tcggcgtgca gacgataggg
     2941 gccgtcgggt gtctcgatcg tcagcgtcgc accatcggcg ccgtgggtga ggccgaccac
     3001 cttgttcttc cagcgcaggt cgatcagcgg cagctcggcg gcacgctgtg cgaggtagcc
     3061 ctcgacgcag tactgctgca ggttgatgaa ggccgggcgc gcgtgaccgg gttcggcgag
     3121 caggtcgaac tgatagatct gcgcgtcgcg gaagaacacc ttgccgacgt tccagcacac
     3181 gcccttcgcg accatgcgct cgccgcagcc gagccggtcg aagatctcca gcgtgcgctt
     3241 ggcgaagcag atggcgcgcg agccggtgga gagcttgtgg tcgttgtcga gcagcagcac
     3301 ctggatgccg cgctgcgcga ggtcgacggc gaggctgagg ccgaccgggc ccgcgccgac
     3361 gacgat
//
LOCUS       DATMJB010000198         2740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_207030, whole genome shotgun sequence.
ACCESSION   DATMJB010000198 DATMJB010000000
VERSION     DATMJB010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207030"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VNU48_04450"
     CDS             complement(<1..255)
                     /locus_tag="VNU48_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10557.1"
                     /translation="MAQTFDRLRQLVAGSAGALLLAAGLLGGSARAQAQTFTLESPDL
                     KSGVFDNKHVLNGFGCNGQNVSPALEWRNVPAGTKSLALQV"
     gene            379..2379
                     /locus_tag="VNU48_04455"
     CDS             379..2379
                     /locus_tag="VNU48_04455"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI10558.1"
                     /translation="MSLKQPRNVGWRISARAVRRDAVSCFLDEPSLRRRISGCPANTN
                     SGDNMILPTRDGPSPFAPREVLVDRAPDGTLRLRSPIPMAAPDWTIVDCLPHWADLAP
                     ERVFLAQRTAAPAWECITYGQTWQRVLAVAEGLVGLGASAGDRLAILSGNSIEHAVIS
                     FAAMSIGVVVAPISPNYTLMPGGLARLAEIAELLKPAFVFAQDQQVFAAARRLEPFAA
                     ATWIVADARTSDIDAAPARTVRLQSLYELKPTPAVEHAWQSIDRDAMAKVLFTSGSTG
                     SPKGVINTHRMMASALQMGALLVAAPDAPVQVEWMPWHHTMGSNVILHGILKCGGSLY
                     IDDGRPVPALMHKTIANLKDVSPTEMFSVPAGYTMLCQAMAHDIALRDSFFRRLRRMT
                     FGGASISRDTLDKLYELSMLATGQRIPVLAGYGATETAPTISVTHWANDRPGEIGLPA
                     PGLELKLVPTGDSYEVRVRGPNVTPGYLGQPGLTAAAFDAEGFYRVGDTVSFIDPDEP
                     RLGLRFTGRIAENFKLSNGTWVAVGSLRATVVDAAQGVLQDAVIAGENRPSCAVLGWL
                     NPAAAQALATGSGGELHLDPGVIEFVRDCLTRLNRTCASSERISAFTLLAEPPSLALG
                     EITDKAYVNQRAVLTNRAAAVERLFASTPHPQVVVVPPGGGM"
     gene            complement(2401..>2740)
                     /locus_tag="VNU48_04460"
     CDS             complement(2401..>2740)
                     /locus_tag="VNU48_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HWI10559.1"
                     /translation="LDTLQHLKNTERMLRETARVGRVGIVSFPNFAHWPNRLHVVAGR
                     MPVTRVLPYQWYDTPNIRVGTYADFEVLARKDGLRILDAFGIQSGRAVRRWPNLRASV
                     AVFKFERAGV"
BASE COUNT          435 a          937 c          914 g          454 t
ORIGIN      
        1 cacctgcagc gccagcgact tggtgcctgc gggcacgttg cgccattcga gcgccggcga
       61 gacgttctgt ccattgcaac cgaagccgtt gagcacgtgc ttgttgtcga acacgccgct
      121 cttcaagtcg gggctttcca gcgtgaacgt ttgcgcctgc gcccgggcgc tgccgccaag
      181 caggccggcc gcgagcagca gcgcgccggc cgagcccgcg acgagttgcc gcaaccgatc
      241 gaatgtctga gccatgaaca ctcctgcagt ggtgggaaga ggatacggcg atccggccgc
      301 gcgcgctcag acgcccgcct tcgccagcac gagtcgcgcg acgcgatcgt cgacacgctc
      361 gcgaccgtcc gtgctcgggt gtcgctgaag cagccgcgca atgtcggctg gcgaatctcc
      421 gctcgtgcgg tgcgccgcga tgcggtatct tgcttcctgg atgagccttc gctgcgccgc
      481 aggatctctg gctgcccggc gaacacgaac agtggagaca acatgattct gccgacacgc
      541 gacgggccct cgcccttcgc tccacgagaa gttctggtcg atcgcgcgcc tgacgggacc
      601 ttgcggttgc gatcgccgat cccgatggcc gcgccggact ggaccatcgt cgactgtctg
      661 ccgcattggg ccgatctggc tcccgaacgt gtctttctcg cgcagcgaac cgcagcgccc
      721 gcatgggagt gcatcaccta cgggcagaca tggcagcgcg tgcttgccgt ggccgaaggc
      781 ctcgtcggtc tcggcgcgag cgccggcgac aggctcgcga tcctctccgg caactccatc
      841 gagcatgcag tcatcagctt cgccgccatg tcgatcggcg tggtggtcgc gccgatttct
      901 cccaactaca cgctgatgcc gggcggtctg gcgcgactgg cggaaattgc cgaactcttg
      961 aagcccgctt tcgtgttcgc gcaggatcag caggtttttg cagccgcccg acggctggag
     1021 ccgttcgcgg cagccacctg gatcgttgcg gacgcaagga cttcggacat cgacgctgcg
     1081 ccggcgcgca cggtccggct ccagtcactg tacgaactga aaccgacacc ggccgtcgag
     1141 catgcgtggc agtccatcga tcgcgacgcc atggccaagg tcctcttcac ctcgggctcg
     1201 acgggctcgc cgaagggcgt gatcaacacg caccggatga tggccagtgc gctgcagatg
     1261 ggtgccctgc tggtggcggc gcccgacgcg ccggtgcagg tggaatggat gccgtggcac
     1321 cacacgatgg gcagcaacgt catcctgcac ggcatcctca agtgcggcgg cagcctgtac
     1381 atcgacgacg gccgaccggt accggcgctg atgcacaaga ccatcgccaa cctgaaggac
     1441 gtctcgccga cggagatgtt cagcgtgcct gccggctaca cgatgttgtg ccaggcgatg
     1501 gcccatgaca tcgcgctgcg cgacagtttc tttcggcggc tgcgtcgaat gaccttcggc
     1561 ggcgcctcga tttcgcgtga cacgctggac aagctctacg aactttcgat gctcgccacc
     1621 gggcagcgga ttccggtgct ggccggctac ggcgccaccg aaacggcgcc gacgatcagc
     1681 gtcacccatt gggcgaacga tcgcccgggg gaaatcggcc tgccggcgcc cggactcgaa
     1741 ttgaagctgg tcccgaccgg cgacagctac gaggtacggg tgcgcggccc caatgtgacg
     1801 ccgggttatc ttggtcaacc cgggttgacg gcggcggcct tcgacgccga gggcttctac
     1861 cgcgtcggcg acacggtgag cttcatcgat cccgacgagc cccgtctggg cctgcgcttc
     1921 accggtcgga tcgcggagaa cttcaagttg tcgaacggca cctgggtcgc cgtgggcagt
     1981 ctgcgcgcga cggtcgtcga tgcagcacag ggcgtgctgc aggacgcggt gatcgcgggc
     2041 gagaaccgcc cgtcctgcgc cgtcctcggc tggctcaatc cggcggccgc acaggcgctg
     2101 gcaaccgggt cgggcggcga acttcatctc gacccgggtg tcatcgagtt cgtgcgcgac
     2161 tgcctgacac gattgaatcg aacctgcgcc agcagcgaaa ggatcagcgc gttcacgctg
     2221 ctggccgagc cgccgtcgct ggctcttggc gagatcaccg acaaggctta cgtgaaccag
     2281 cgcgccgtcc tcacgaaccg cgccgcggcg gtcgagcgac tgttcgcctc cacgccgcat
     2341 ccgcaagtgg tcgtcgtgcc gccgggcggc gggatgtgat gacgatcgag ccagccgggc
     2401 tcacaccccc gcgcgctcga acttgaacac cgccacgctc gcgcgcaggt tcggccagcg
     2461 ccgcaccgcg cgcccgctct ggatgccgaa ggcgtcgagg atgcgcagac cgtccttgcg
     2521 cgccagcacc tcgaagtcgg cataggtgcc gacgcggatg ttcggcgtgt cgtaccactg
     2581 gtacggcagc acgcgcgtca ccggcatgcg cccggccacc acgtgcagcc ggttcggcca
     2641 gtgcgcgaag ttcgggaagc tgacgatgcc gacgcggccg acgcgcgccg tctcgcgcag
     2701 catccgctcg gtgttcttca ggtgctgcag ggtgtcgagc
//
LOCUS       DATMJB010000199         2229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_811954, whole genome shotgun sequence.
ACCESSION   DATMJB010000199 DATMJB010000000
VERSION     DATMJB010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811954"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..266)
                     /locus_tag="VNU48_04465"
     CDS             complement(81..266)
                     /locus_tag="VNU48_04465"
                     /inference="COORDINATES: protein motif:HMM:NF023508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3563 family protein"
                     /protein_id="HWI10560.1"
                     /translation="MFSLFQSIKNLFNVSVSPAQDRDDQYLAEATDIYDLERRMRQVD
                     SGRHNLYAIGSYGIFMR"
     gene            474..>2229
                     /locus_tag="VNU48_04470"
     CDS             474..>2229
                     /locus_tag="VNU48_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI10561.1"
                     /translation="MFMLFEKLVQPYPDAEPAPPPRRFFPFLWACTRGLRPYMIAMTL
                     CTAVIGAFEALLFAFLGRIVDWLSATTPARLWADQGHHLVLLGGVLTGSIALVAMQSL
                     LKQQALAGNFPMLLRWNFHRLMLAQSMHFYQDEFAGRISTKVMQTALAVRDAWMILAE
                     LMVFVVIYFVTIMAMLGGFDRWLLAPFTGWLLLYGAALWHFVPRLGRVAKAQADARSM
                     MTGRITDAYTNIATVKLFSHSRREAGYARSAMQEFLGTVHSQMRLVTAFEIVNHALSM
                     ALIASTAGAALWLWTQAQVGVGALAAATAMALRLNGISHWVMWEMASLFEHIGTVQDG
                     INTLSRPHAVVDRADAKPLQVRLGDVRFEHVSFAYGGTSRVIDDLSLHIRPGEKVGLV
                     GRSGAGKSTIVNLLLRLYDVDAGRVLIDGQDIAQATQDSLRAQVGMVTQDTSLLHRSV
                     RDNILYGRPDAGDAQMVVAARRAEAHEFIQGLSDPKGRKGYDAHVGERGVKLSGGQRQ
                     RIAIARVMLKDAPILLLDEATSALDSEVEAAIQASLYKLMEGKTVVAIAHRLSTIAAM
                     DRLIVLDQGRIVEQGDHRG"
BASE COUNT          337 a          755 c          774 g          363 t
ORIGIN      
        1 cgggcccagc gccgggccgc tcccaagcgg gcccgagacc ccctcggggg gtgctgcggt
       61 acaccgcagc gggggctcca tcacctcatg aagatgccgt aggagccgat cgcgtacagg
      121 ttgtggcggc ccgagtcgac ctggcgcatg cggcgctcga ggtcatagat gtcggtggcc
      181 tcggccaggt actggtcgtc gcgatcctgg gccgggctca ccgacacgtt gaacaggttc
      241 ttgatcgact ggaaaaggga gaacatgatg gtctctggag tggttggagc ccgtagtcta
      301 agggaaaacc ctaggccatg cactgggcag gggcagactt cagaacggag gcacctccag
      361 cgggcgatga gtcacgtggt ggcgccacct tggtgcttga tcgtctgccg caccccgcgc
      421 tcccggatcg gctaacctcg ccgaccttgc ccgaaccccg ccggagatcc cgcgtgttca
      481 tgctcttcga aaagctcgtg cagccctacc cggatgccga gcccgcgccg ccaccccgac
      541 gcttcttccc tttcctgtgg gcctgcaccc gcggcctgcg cccgtacatg atcgcgatga
      601 ccctgtgcac cgcggtcatc ggggcgttcg aggcgctgct gttcgcattt ctcggccgca
      661 tcgtcgactg gctgagcgcg acgacgccgg cacgcctgtg ggccgaccag ggccaccacc
      721 tcgtgctgct gggcggcgtg ctgaccggca gcatcgcgct ggtcgcgatg cagtcgctgc
      781 tgaagcagca ggccctggcc ggcaacttcc cgatgctgct gcgctggaac ttccatcggc
      841 tgatgctcgc gcagagcatg cacttctacc aggacgagtt cgccggccgg atctcgacca
      901 aggtgatgca gaccgcgctg gcggtgcgcg atgcgtggat gatcctggcc gagctgatgg
      961 tgttcgtggt catctacttc gtcaccatca tggcgatgct tggcggcttc gaccgctggc
     1021 tgctcgcgcc gttcaccggc tggctgctgc tgtacggcgc ggcgctgtgg catttcgtgc
     1081 cgcggctggg ccgcgtggcg aaggcgcagg ccgatgcgcg ctcgatgatg accggccgca
     1141 tcaccgacgc ctacaccaac atcgccaccg tcaagctgtt ctcgcactcg cggcgcgagg
     1201 ccggctacgc gcgctcggcg atgcaggagt tcctcggcac cgtgcactcg cagatgcgcc
     1261 tggtgacggc gttcgagatc gtcaatcacg cgctgagcat ggcgctgatc gcgagcaccg
     1321 ccggcgccgc gctgtggctg tggacgcagg cccaggtcgg cgtcggtgcg ctcgccgcgg
     1381 ccaccgcgat ggcgctgcgc ctgaacggca tctcgcactg ggtgatgtgg gagatggcgt
     1441 ccctcttcga gcacatcggc acggtgcagg acggcatcaa caccttgagc cgcccgcatg
     1501 cggtggtcga tcgcgccgac gcgaagccgc tgcaggtgcg cctgggcgac gtgcgcttcg
     1561 agcatgtgag cttcgcgtac ggcggcacgt cgcgcgtgat cgacgacctg tcgctgcaca
     1621 tccggccggg cgagaaggtc ggcctggtcg gccgctcggg cgccggcaag agcacgatcg
     1681 tgaacctgct gctgcgcctg tacgacgtcg acgccggccg cgtgctgatc gacggccagg
     1741 acatcgccca ggcgacgcag gactcgctgc gcgcgcaggt cggcatggtc acgcaggaca
     1801 cgtctctcct gcaccgatcc gtgcgcgaca acatcctgta cggccggccc gatgcgggcg
     1861 acgcgcagat ggtcgtcgcc gccaggcgcg ccgaggcgca cgagttcatc caggggctgt
     1921 ccgacccgaa gggccgcaaa ggctacgacg cgcatgtcgg cgagcgcggc gtcaagctct
     1981 cgggcgggca gcgccagcgc atcgcgatcg cgcgcgtgat gctgaaggac gcgccgatcc
     2041 tgttgctcga cgaggcgacg agcgcgctcg actcggaagt cgaggccgcg atccaggcca
     2101 gcctgtacaa gctgatggaa ggcaagaccg tggtcgcgat cgcgcaccgg ctgtcgacca
     2161 tcgccgcgat ggaccggctg atcgtgctcg accagggccg catcgtcgag cagggcgatc
     2221 atcgcggcc
//
LOCUS       DATMJB010000200         2473 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_726568, whole genome shotgun sequence.
ACCESSION   DATMJB010000200 DATMJB010000000
VERSION     DATMJB010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726568"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1374
                     /locus_tag="VNU48_04475"
     CDS             <1..1374
                     /locus_tag="VNU48_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HWI10562.1"
                     /translation="SIEKSELLSDFLKQEGVPHEVLNARQHEREAHIVAQAGRKGVVT
                     IATNMAGRGTDIVLGGNVENQIKFIDADAELDEAEKARRARQLQDEWQSLHEKVKAEG
                     GLRIIATERHESRRIDNQLRGRSGRQGDPGSSRFYLSLDDSLMRIFAGDRVRAIMERL
                     KMPDGEAIEAGIVTRSIESAQRKVEARNFDVRKQLLEYDDVSNDQRKVIYQQRNDILE
                     AASLNEQIANLRESTMSDVVRTFVPADSVEEQWDLDGLEKVLREEWHVDLALKTEVEK
                     SASITDEEIVEKVARAADAVFDEKVQRVGLEQFTPFMRMILLQSIDSHWREHLAALDY
                     LRQGIHLRGYAQKNPKQEYKREAFELFSQLLDVVKMDVTRVLMTVRIQSQEEVAQAAE
                     AIEERAERISNVTYTHPNEDGSVSSDGREAADLATLAAAVPKVGRNDPCPCGSGKKYK
                     HCHGRLA"
     gene            1510..>2473
                     /locus_tag="VNU48_04480"
     CDS             1510..>2473
                     /locus_tag="VNU48_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWI10563.1"
                     /translation="MQLALHNSARSVWLQFAAVVVLVLFGFSSSRPVAGAVAGIVGTG
                     VAIWRLLISRRYGDPEDLSERGLRQLEFQLEGNAALAGVMWAIGTFGIYPALLGTAGT
                     TYVGMVLGSITVAAFFMTLVGRSFEILSAIQVGSLVVVSLTSEPVRSLSVAALALIFG
                     LTVYRASAQFKATATRAIRHGQEADQATLSLQRAKEIAESANIAKSQFLATMSHEIRT
                     PMNGVLGALELLRRSPLDPDQRRLVRTASSSGASLMAILNDVLDHSKIEAGKLTLVDA
                     PLSLHAMALSVISLFRGNAEAKGLQLSLEIDPEVENWVIGDAQRL"
BASE COUNT          462 a          759 c          844 g          408 t
ORIGIN      
        1 tcgatcgaga agtcggaatt gctgtccgac ttcctcaagc aggaaggcgt gccgcacgag
       61 gtgctcaatg cccgccagca tgagcgcgag gcgcacatcg tcgcccaggc cgggcgcaag
      121 ggcgtggtga cgatcgccac caacatggcc ggccgcggta cggacatcgt gctcggcggc
      181 aacgtcgaga accagatcaa gttcatcgac gccgacgccg agctggacga ggccgagaag
      241 gcgcgccgcg cccggcagct gcaggacgag tggcagagcc tgcacgagaa ggtgaaggcc
      301 gaaggcggcc tgcgcatcat cgcgaccgaa cgccacgagt cgcgccgcat cgacaatcag
      361 ctgcgcggcc gctcgggccg ccagggcgac ccgggctcgt cgcgcttcta cctgtcgctc
      421 gacgactcgc tgatgcgcat cttcgccggc gaccgggtgc gcgcgatcat ggaacggctg
      481 aagatgcccg acggcgaggc gatcgaggcc ggcatcgtca cgcgcagcat cgagagcgcg
      541 cagcgcaagg tcgaggcgcg caacttcgat gtgcgcaagc aactgctcga gtacgacgat
      601 gtcagcaacg accagcgcaa ggtgatctat cagcagcgca acgacatcct cgaggccgcg
      661 agcctcaatg agcagatcgc caacctgcgc gagagcacga tgagcgacgt ggtgcgcacc
      721 ttcgttccgg ccgacagcgt cgaggagcag tgggacctgg acggcctcga gaaggtgctg
      781 cgcgaggagt ggcatgtcga cctggcgctg aagaccgagg tcgagaagag cgcgtccatc
      841 accgacgagg agatcgtcga gaaggtggcc cgcgccgccg atgcggtgtt cgacgagaag
      901 gtgcagcgcg tcgggctcga gcagttcacg ccgttcatgc gcatgatcct gctgcagtcg
      961 atcgactcgc actggcgcga gcacctggcc gcactcgact acctgcgcca gggcatccac
     1021 ctgcgcggct atgcgcagaa gaacccgaag caggaataca agcgcgaggc cttcgagctc
     1081 ttcagccagc tgctcgacgt cgtcaagatg gacgtgacgc gcgtgctgat gacggtgcgc
     1141 atccagtcgc aggaagaggt cgcgcaggcg gccgaggcga tcgaggagcg cgccgagcgc
     1201 atcagcaacg tcacctacac gcacccgaac gaggacggca gcgtgtcgtc ggacggtcgc
     1261 gaggcggccg atctcgccac gctcgcggcg gcggtgccca aggtcgggcg caacgacccg
     1321 tgcccgtgcg gcagcggcaa gaagtacaag cactgccacg ggcgtctggc ctgagcgggc
     1381 tcggatgcgg ccgcggcggc ccgtttcgag gcgaagatgc cgacgagagg gcagaacaga
     1441 acccgcacgt tatgcttgca gcctatgaca gatgaggcgt caacgaagtt ggctgtacaa
     1501 cgtgagctct tgcagctcgc gctgcacaac tcggcgcgaa gcgtgtggtt gcagttcgct
     1561 gcggttgtcg tcctcgttct attcggtttc agcagcagcc ggcccgttgc cggagcggtg
     1621 gcgggcatcg tcgggacagg ggtcgccatc tggcggctgc tgatttccag gcgctatggt
     1681 gacccggaag atctctccga gcgaggattg cgccagctcg aattccagct cgagggaaac
     1741 gcggcgttgg cgggcgtcat gtgggcaatc ggtacgttcg ggatttatcc ggcattgctt
     1801 ggcaccgcgg gaaccaccta cgtcgggatg gttctggggt cgatcaccgt ggcggcattc
     1861 ttcatgaccc tggtggggcg ttcgttcgag atcctgtccg ccatccaggt tggctcgttg
     1921 gttgtcgtca gtctgacgag cgaaccggtg cgctcgctgt ctgtcgccgc tctggcgctg
     1981 attttcggtc ttacggtcta ccgtgcgtcg gcgcagttca aggctaccgc gacccgcgca
     2041 atccgccacg ggcaggaagc agatcaggca acgttgtcgc tgcagcgggc caaggaaatc
     2101 gccgaatcag ccaacatcgc caagtcgcaa ttcctggcca cgatgagcca tgaaattcgg
     2161 accccgatga acggtgtgtt gggggcgctc gaactcctgc gccgctctcc gctcgatcca
     2221 gaccagcgcc ggctggttcg gacggcttcg tcgtcgggcg cgtcgctgat ggcaatcctg
     2281 aatgacgtgc ttgatcactc caagatcgag gcgggcaaac tcacgctggt cgatgccccg
     2341 ctgtcgttgc acgccatggc cttgtcggtg atttccttgt ttcgcggaaa tgcggaagcc
     2401 aaggggctac agctttcgct cgagattgat ccggaagtcg aaaactgggt cataggcgac
     2461 gcgcagcgtt tga
//
LOCUS       DATMJB010000201         4949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_676072, whole genome shotgun sequence.
ACCESSION   DATMJB010000201 DATMJB010000000
VERSION     DATMJB010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676072"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /gene="msrA"
                     /locus_tag="VNU48_04485"
     CDS             complement(<1..727)
                     /gene="msrA"
                     /locus_tag="VNU48_04485"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWI10564.1"
                     /translation="MPELSPTKLPRRRFAAALTGLVAVLAIASAPLNSRAGEAVVKIP
                     ALVGDTAAGASAGPETAVFAGGCFWGVQGVFQHTRGVLSAVSGYAGGSQATAHYDLVG
                     SGLTGHAESVQVRFDPKQVSYAQLLQIFFSVAHDPTQLNRQGPDRGTQYRSALFTLDA
                     TQQQTAQRYIAQLDAAKVYPAKIVTQVAPLAAFYPAEDYHQDYATLHPESGYIATFDL
                     PKIAALKNLFAAQYRADPVLVAGA"
     gene            complement(851..1198)
                     /locus_tag="VNU48_04490"
     CDS             complement(851..1198)
                     /locus_tag="VNU48_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10565.1"
                     /translation="MDLKLPIRIFDELSESVIESTGMLDLASGEIRSVEYDDYDAELS
                     GLPAEDEHYEFTSGLLTNGTKDVEFRVEVDVMSGHYSVTPSELLELKGRAAKLFTAAP
                     TDAAPTIAPRKRR"
     gene            complement(1288..3174)
                     /locus_tag="VNU48_04495"
     CDS             complement(1288..3174)
                     /locus_tag="VNU48_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWI10566.1"
                     /translation="MVTRQVVTPVQLLEAIERQAKMPMVRIGEALVSLGMIDETQLQE
                     ALAQQRSDRSVPLGELLVRMGVVSREDLQIALSRKMGYPLVDVDSFPVEAEALRRLSY
                     SVAMRLQVLPLLLRDGRLIVALDDPARRRSAVDEVEFSAQMKVVPVLANSRSIDAVLH
                     AAYEKIGAVDGPRSPAHDAALSMKLDLPDTGKLVETLEREGLERASDDDRPIEQSDNS
                     LVRLLNNMIIEADREGVSDIHIESYPGREKIRVRFRKDGVLRTYLELPPNYRNALIAR
                     VKIMCELDISERRKPQDGKINFAKFSPQHKLELRVATIPTNHGLEDVVLRLLSSAKPM
                     PVDRLGLSPDNLARLKHAVERPYGMVLCVGPTGSGKTTTLHSALSHINVPERKIWTAE
                     DPIEITQPGLRQVQVNPKIEWTFAKALRAFLRADPDVIMVGEIRDQETANMVVEASLT
                     GHLVLSTLHTNSAPETVTRLLDMGLDPFNFGDSLLAVLAQRLVRRLCTHCRTTQPATR
                     EQADELLADYMLGFGVEPPIDRDTVMAGWMQRNSRDGRLMLYRSPGCKECGDSGFKGR
                     AGIHEMMVVSRELRRLIQTNAPAEAIQRVALTEGMRTLRQDGIDKVLTGITSIEEVRA
                     TSNV"
     gene            complement(4018..>4949)
                     /locus_tag="VNU48_04500"
     CDS             complement(4018..>4949)
                     /locus_tag="VNU48_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HWI10567.1"
                     /translation="KMSLPELARRGYPDRMAIGSLAGAGTLGLLIPPSIIMIVYGVTA
                     DVSISQLFIGGVIPGVLLALIFSIYIAFWSLTHKALIPPADAPMSFAQRLHESRHLIP
                     VVLLIAAVLGSIYAGIATATEAAGLGVVGSLILSLLQGSMSWQTFKDSLMGGTRLYCM
                     IALILAGAAFLTLSMGYIGLPRHLAEWIGGLGLSKFQLIMALTAFYVVLGCFLDGISM
                     VVLTMGVIMPTVQKAGIDPLWFGIFIVIVVEMAQVTPPVGFNLFVLQGMTQRSIVYIA
                     RAAFPFFLLMVAMVVLLYAFPQVVTFLPLQMKS"
BASE COUNT          732 a         1673 c         1763 g          781 t
ORIGIN      
        1 cggcgccggc caccagcacc gggtcggccc ggtactgcgc cgcgaacagg ttcttcaggg
       61 ccgcgatctt cggcaagtcg aaggtcgcga tgtagccgga ctcggggtgc agcgtcgcgt
      121 agtcctggtg gtagtcctcg gccgggtaga acgcggcgag cggcgcgacc tgcgtgacga
      181 tcttcgccgg gtagaccttg gccgcgtcga gctgcgcgat gtagcgctgc gcggtctgct
      241 gctgcgtcgc atccagcgtg aacagcgccg agcggtactg cgtgccgcgg tcggggccct
      301 ggcggttcag ctgggtcggg tcgtgcgcga ccgagaagaa gatctgcagc agctgcgcgt
      361 acgacacctg cttcgggtcg aagcgcacct gcaccgattc ggcatggccg gtcagccccg
      421 agccgacgag gtcgtagtgc gcggtggcct ggctgccgcc ggcatagccc gacaccgcgc
      481 tgagcacgcc gcgggtgtgc tggaacacgc cctgcacgcc ccagaagcag ccgccggcga
      541 agaccgcggt ctcgggaccg gcgcttgcgc cggcggccgt gtcgcccacc agcgccggga
      601 tcttcaccac ggcttcgccg gcgcggctgt tgagcggcgc cgaggcgatc gcgagcacgg
      661 cgacgaggcc cgtcagtgcg gccgcgaagc ggcggcgcgg cagtttcgtc ggggaaagtt
      721 cgggcattcg gttcatgggc ggctcctgca cggattgtcg agtccgctgg aggtcgcgag
      781 ccgggccgcg cttcttacac cctggcgctt gcagggcggg gtcgtctcag cgccgcttgc
      841 gccgtccgtt tcagcgccgc ttgcgcggcg cgatcgtcgg cgcggcgtcg gtcggcgccg
      901 cggtgaacag cttcgcggcg cggcccttca gctcgagcag ttcgctgggc gtgaccgagt
      961 agtggcccga catcacgtcg acctcgacgc ggaattcgac atccttcgtg ccgttcgtca
     1021 gcagccccga ggtgaactcg tagtgctcgt cctcggccgg caggcccgac agttcggcgt
     1081 cgtagtcgtc gtactcgacg ctgcggatct cgccgctggc caggtcgagc atgccggtcg
     1141 attcgatcac cgattccgag agttcgtcga atatcctgat cggcagcttc agatccacgt
     1201 caaatccttc gaggcacgcg ccacgcgcgc agcctgaagt ttaagggccc acctcggggc
     1261 gcccgacggc gacggccggg acgaggctca gacgttgctc gtcgcgcgca cctcttcgat
     1321 cgaggtgatg ccggtcagca ccttgtcgat gccgtcctgg cgcagcgtgc gcatgccttc
     1381 ggtcaacgcg acgcgctgga tcgcctcggc gggcgcgttg gtctggatca ggcggcgcag
     1441 ctcgcgcgac accaccatca tctcgtggat gccggcgcgg cccttgaagc cgctgtcccc
     1501 gcactccttg cagccgggcg agcggtacag catcaggcgg ccgtcgcggc tgttgcgctg
     1561 catccagccg gccatcacgg tgtcgcggtc gatcggcggc tcgacgccga agcccagcat
     1621 gtagtcggcg agcagttcgt cggcctgctc gcgcgtcgcc ggctgcgtcg tgcggcagtg
     1681 cgtgcacagg cgccgcacca ggcgctgcgc cagcaccgcg agcagcgagt cgccgaagtt
     1741 gaacgggtcc agccccatgt cgagcaggcg cgtcacggtt tcgggcgcgc tgttggtgtg
     1801 cagcgtgctc agcaccaggt ggccggtgag cgaggcctcg acgaccatgt tcgcggtctc
     1861 ctggtcgcgg atctcgccga ccatgatcac gtccgggtcg gcgcgcagga aggcgcgcag
     1921 cgccttcgcg aaggtccact cgatcttcgg gttgacctgc acctggcgca ggcccggctg
     1981 ggtgatttcg atcgggtcct cggcggtcca gatcttgcgc tcgggcacgt tgatgtggct
     2041 cagcgccgag tgcagcgtgg tggtcttgcc cgagccggtc gggccgacgc acagcaccat
     2101 gccgtagggc cgttcgaccg cgtgcttcag gcgcgccaga ttgtcgggcg acaggccgag
     2161 ccggtccacc ggcatcggct tggccgagct cagcaggcgc agcaccacgt cttcgaggcc
     2221 gtgattcgtc gggatcgtcg cgacgcgcag ctcgagcttg tgctgcggcg agaacttcgc
     2281 gaagttgatc ttgccgtcct ggggcttgcg ccgctcggag atgtcgagct cgcacatgat
     2341 cttgacgcgc gcgatcaggg cgttgcggta gttcggcggc agctcgaggt aggtgcgcag
     2401 cacgccgtcc ttgcggaagc gcacgcggat cttctcgcgc cccgggtagc tctcgatgtg
     2461 gatgtcggag acgccctcgc ggtcggcctc gatgatcatg ttgttgagca gccgcaccag
     2521 cgagttgtcg gactgctcga tcgggcggtc gtcgtcgctg gcgcgttcca ggccttcgcg
     2581 ttcgagcgtc tcgaccagct tgccggtgtc gggcaggtcg agcttcatcg acagcgccgc
     2641 gtcgtgcgcc gggctgcgcg ggccgtcgac cgcgccgatc ttctcgtagg ccgcgtgcag
     2701 cacggcgtcg atcgagcggc tgttcgcgag caccggcacg accttcatct gcgcgctgaa
     2761 ctcgacctcg tcgaccgcgc tgcggcggcg cgcggggtcg tcgagcgcga cgatcaggcg
     2821 gccgtcgcgc agcagcagcg gcagcacctg cagccgcatc gcgaccgaat agctcaggcg
     2881 ccgcagcgcc tcggcctcga ccgggaagct gtcgacgtcg accagcgggt agcccatctt
     2941 gcgcgacagc gcgatctgca ggtcctcgcg cgagaccacg cccatgcgca ccagcagttc
     3001 gcccagcggc acgctgcggt cgctgcgctg ctgcgcgagc gcctcctgca gttgcgtctc
     3061 gtcgatcatg ccgagcgaga ccagcgcctc gccgatgcgc accatcggca tcttcgcctg
     3121 gcgctcgatc gcttcgagca gctgcaccgg cgtgacgacc tggcgcgtca ccagcacgtc
     3181 gccgagcttg cgcccgcgca gctgctgctg cacgcccgcg gcctcctcga tctgcgctgg
     3241 cgtcgcgctg tgctgttcga ccagcagctc gccgatgctg gagccgatct cgaactggtc
     3301 gtaggcgttg cgcggcacga agcagcgcag cacgctgccg tcgtcgtcgg ccgaatcggc
     3361 ctcggcgcag gggtggaaca ggaacagccc gtacgcgttc tcgacgtggc cgatggtctc
     3421 gccggtcagc tcgacgccgc tggccagcgt gatctggtac agcgagcgcg cgcggtgccc
     3481 gagcaggccg gcgtgcgggt cgccgagcgc gagcgcgccc ggccgcagcg gcgtggtcag
     3541 ggtcagcgag cggaactggt cgaagcgcag cggcagcgtc gtgcgcgccg ggggcagctg
     3601 gacgtgggcg acaccttcct cgggcacgaa gaaggtcagc cggcccttgg tgcggctgtc
     3661 gttcagcccg acgatctcgc agggcagcgg gtcggtctgc ggcgtggccg gcgggtagct
     3721 ggcgtagggc ggcgtgggcc agcagaagac atcgctgatg gccttgtcgg aagcggcgct
     3781 gcgcccgccg gacgcgccgc tcgcgaacgc gtccaggtgc tcgggggcct tcgcccattc
     3841 caggggggtc aacatatcgg ccatcgcggt gtccttgcaa cggtcggccg cacgttcacc
     3901 cagcgggcgc gcgtcggcca acacttcttc acaggggcga tggtcgcgtc gaatgcccga
     3961 ctgcgatgag cggaaatgcg ggccttgggc ccgccagttc aaggcttccg gtcgcgctca
     4021 ggacttcatc tgcagcggca ggaaggtgac gacctgcggg aaggcgtaca gcagcacgac
     4081 catcgcgacc atcagcagga agaacgggaa cgcggcccgc gcgatgtaga cgatgctgcg
     4141 ctgggtcatg ccctgcagca cgaacaggtt gaagcccacc ggcggcgtga cctgcgccat
     4201 ctcgacgacg atgacgatga agatgccgaa ccacagcggg tcgatgccgg ccttctgcac
     4261 cgtcggcatg atcacgccca tggtcagcac gaccatcgag atgccgtcca ggaagcagcc
     4321 gagcacgacg tagaacgcgg tcagcgccat gatcagctgg aacttggaga gccccagacc
     4381 gccgatccat tcggccaggt gccgcggcag cccgatgtag cccatgctca gggtcaggaa
     4441 ggccgcgccg gccaggatca gcgcgatcat gcagtacagc cgcgtgccgc ccatcagcga
     4501 atccttgaag gtctgccagc tcatcgagcc ctgcagcagc gacaggatca gcgagccgac
     4561 cacgcccagc ccggcggcct cggtggccgt ggcgatgccg gcgtagatcg agcccagcac
     4621 cgcggcgatc agcagcacca ccggaatcag gtggcgcgac tcgtgcagtc tctgcgcgaa
     4681 gctcatcggc gcgtcggccg gcgggatcag cgccttgtgg gtcagcgacc agaacgcgat
     4741 gtagatcgag aagatcagcg ccagcagcac gcccgggatc acgccgccga tgaacagctg
     4801 cgagatcgac acgtcggcgg tgacgccgta gacgatcatg atgatcgacg gcgggatcag
     4861 caggcccagc gtgccggcgc cagccagcga gccgatcgcc atgcggtcgg gatagccacg
     4921 ccgggcgagc tccggcaggc tcatcttgc
//
LOCUS       DATMJB010000202         2126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_74661, whole genome shotgun sequence.
ACCESSION   DATMJB010000202 DATMJB010000000
VERSION     DATMJB010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74661"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..2029
                     /locus_tag="VNU48_04505"
     CDS             101..2029
                     /locus_tag="VNU48_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWI10568.1"
                     /translation="MSEFSSSGLFDRFKAVMAAAPNMVPEVTATFARLVGTSAEYRSG
                     PWWLLLRIGIVVALVALGIAGLRRLEAGLRPIAYGEAGTYGRRTRIMLAVVLDLVHRA
                     LPIAFVYALLEFWLRPEILQQRLILRTLATVSFWYLLTFPVDAALRPRHPHLRLVRFD
                     DATARVASFHLYGILLAGTASIHYLQMLIHNGTSIPSAQFLALVIAVVVGAWTVLAIR
                     AVRATQLRGQPGTRAAWPYGVGALAALVWLTWVIGVLNVDLAHFDALVWSIVVVAAAW
                     VVDATIGLAIGRQVNPDARVRWWRDVGVLRVLRRFVRVAAFVVIGVLIVQTWIVELFG
                     IVSGERWRQIKAGMTEAGVIVVIGYLLWEALRIFVDRKLAHAAPSTGVPVDAEEEIRA
                     GSRFQTMLPIIRIFAGIGIGAVTVLFALSQLGMNIGPLIAGASIFGLAISFGSQTLVR
                     DIVSGIFFMADDAFRVGEYVDTGKLKGTVEGISMRSIKLRHQNGQIHTIPFGQIQSVT
                     NFSRDWATVKFNLRLARDTDLEKVRKLVKKVGTAMLEDADLKPEFIQPLKLQGVADIE
                     PGAMVVRCKFTVRPLKPSWVQREALKRLFRAFGENGVQFASNAVTVQTLGGGAFDPTA
                     AGAAVTSSTPQPAAAGGG"
BASE COUNT          273 a          735 c          764 g          354 t
ORIGIN      
        1 gctcgcgcgc gacgcgtcct cccatcccgc tgggtccggc gcggccgcgc ccgccgccct
       61 ggccgcgccg acgacagggc gagggctcga taccgcgccg atgagcgagt tcagctcgag
      121 cggcctgttc gatcgcttca aggcggtgat ggcggccgcg cccaatatgg ttcccgaggt
      181 gacggcgacc ttcgcccggc tggtcgggac ctccgccgaa taccgcagcg gaccctggtg
      241 gctgcttctg cggatcggga tcgtcgtcgc gctggtcgcg ctcggcatcg cggggctgcg
      301 gcggctcgag gccgggttgc ggccgatcgc gtacggcgaa gcgggcacgt acggccgtcg
      361 tacgcggatc atgctcgcgg tcgtgctcga cctcgtgcac cgcgcgctgc cgatcgcctt
      421 cgtttacgcg ctgctcgagt tctggctgcg tccggaaatc ctgcagcagc ggctgatcct
      481 gcgcacgctg gcgacggtct cgttctggta cctgctgacc ttcccggtcg acgccgcgct
      541 gcggccgagg cacccgcatc tgcgcctggt ccgcttcgac gacgcgaccg cgcgcgtcgc
      601 ctcgttccat ctctacggca tcctgctcgc cggcaccgcg tcaatccact acctgcagat
      661 gctgatccac aacggcacct cgattccctc ggcgcagttc ctcgcgctgg tgatcgcggt
      721 cgtggtcggc gcgtggacgg tgctggcgat ccgcgccgtg cgcgccacgc agctccgcgg
      781 ccagccgggg acgcgggcgg cttggcctta cggcgtcggg gcgctggcgg cgctggtctg
      841 gctcacctgg gtgatcggcg tgctcaacgt cgacctcgcg catttcgacg cgctggtgtg
      901 gtcgatcgtc gtggtcgccg cggcgtgggt ggtcgacgcc accatcggcc tggccatcgg
      961 gcggcaggtc aatcccgacg cgcgcgtgcg ctggtggcgc gacgtcggcg tgctgcgcgt
     1021 gctgcgccgc ttcgtgcgcg tcgcggcgtt cgtggtcatc ggcgtgctga tcgtccagac
     1081 ctggatcgtc gagctgttcg gcatcgtcag cggcgagcgc tggcggcaga tcaaggccgg
     1141 gatgaccgag gccggcgtga tcgtggtgat cggctacctg ctctgggagg cgctgcgcat
     1201 cttcgtcgac cgcaagctcg cgcatgcggc gccgtcgacc ggcgtgcccg tcgacgcgga
     1261 ggaggagatc cgggccggct cgcgcttcca gaccatgctg ccgatcatcc gcatcttcgc
     1321 cggcatcggc atcggcgcgg tcaccgtgct gttcgcgctg tcgcagctcg gcatgaacat
     1381 cgggccgctg atcgccggcg cctcgatctt cggcctcgcc atcagcttcg gctcgcagac
     1441 gctggtgcgc gacatcgtct cgggcatctt cttcatggcc gacgacgcgt tccgcgtcgg
     1501 cgagtacgtc gacaccggga agctgaaggg cacggtcgag ggcatctcga tgcgctcgat
     1561 caagctgcgg caccagaacg ggcagatcca caccatcccg ttcgggcaga tccagtcggt
     1621 gaccaatttc agccgcgact gggcgacggt gaagttcaac ctgcgcctcg cgcgcgacac
     1681 cgacctcgag aaggtgcgca agctggtcaa gaaggtcggc acagcgatgc tcgaggatgc
     1741 cgacctcaag cccgagttca tccagccgct caagctgcag ggcgtggccg acatcgagcc
     1801 cggcgcgatg gtggtgcgct gcaagttcac ggtgcggccg ttgaagccga gctgggtgca
     1861 gcgcgaggcg ctcaagcggc tgttccgcgc cttcggcgag aacggcgtcc agttcgcgag
     1921 caacgccgtc accgtgcaga cgctgggcgg cggagcgttc gatccgactg cggcgggggc
     1981 ggcggtcacg tcgtcgacgc cgcagccggc ggcggccggc ggcggctgat caggcgggcg
     2041 cgcggcgctt ctggaccagc gcccagagct gcgggccgag caccagcgcg agcgcgatca
     2101 ccagcagcgt cagcgacagc ggcttg
//
LOCUS       DATMJB010000203         5699 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_666937, whole genome shotgun sequence.
ACCESSION   DATMJB010000203 DATMJB010000000
VERSION     DATMJB010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666937"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VNU48_04510"
     CDS             complement(<1..415)
                     /locus_tag="VNU48_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWI10569.1"
                     /translation="MLANDILHTIGNTPHIRINRLFGGTHSVYIKSERANPGGSIKDR
                     IALAMVEAAEASGALKPGGTIIEPTSGNTGVGLAMVAAVKGYRLILVMPDSMSIERRR
                     LMLAYGARFELTPREKGMNGSIARANELLAQIPGGW"
     gene            484..1554
                     /gene="hemE"
                     /locus_tag="VNU48_04515"
     CDS             484..1554
                     /gene="hemE"
                     /locus_tag="VNU48_04515"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HWI10570.1"
                     /translation="MHAPLRNDSFLRACLRQPTTHTPVWLMRQAGRYLPEYRATRAKA
                     GSFMGLATNPDFATEVTLQPIDRYALDAAIVFSDILTVPDAMGLGFSLDAGEGPRFAH
                     PVRDEAAVAKLAVPDMNKLRYVFDAVTSVRRALDGRVPLIGFSGSPWTLACYMVEGAG
                     SNDYRLVKTMLYSRPDLMHRILEVNAAAVAQYLNAQVEAGAQAVMIFDSWGGVLADGA
                     FQQFSLAYTRRVLAQLKREHEGQRIPQIVFTKGGGPWLEEIAAAQPDVVGLDWTVNLG
                     AARARIGDRVALQGNIDPNVLFAEAAQIRAEVARTLTSFGAGPGHIFNLGHGISQHTP
                     PENVAVLVDAVHELSAGPCAQP"
     gene            1946..4012
                     /locus_tag="VNU48_04520"
     CDS             1946..4012
                     /locus_tag="VNU48_04520"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HWI10571.1"
                     /translation="MRVAVDAPQHTGLSAPLDYTSERPLPAGTLVSVPLGRREVPGVV
                     WHGAPDAATTVTLRPVRAALTSLPPLGAAWLELVDFAAAYYQRSTGEIALSVLPPELR
                     KLDDAQLANRIKKLHKALGAPADAARPTAAPALTDDQARALATIAAAGTQPMPAPLLL
                     HGVTGSGKTEVYLGAAAQALAAGRQALVLVPEINLTPQLEARFGERFAGRHIVSLHSA
                     LTPAQRLRSWLAAHLGLADLVLGTRLAVFASMPRLGLIVVDEEHDPSYKQQEGARYSA
                     RDLAVYRGRLERACVVLGSATPSLESWQRAAEGRYTRLALPSRIGGGALPAVRLVDMN
                     QQPKTRGPATALSAPLVAALQERLARGEQSLVFLNRRGYAPVLHCGECGWKSGCPHCS
                     AWRVFHKLDRTLRCHHCGYAERVPRACPDCGNPDIAPIGRGTERLEEQLGALLRTPDD
                     RPARIARIDADSTRLKGALEAQLGAVHAGDVDVLVGTQMVTKGHDFRRITLVAAVNPD
                     SSLFSSDFRAPERLFALLMQAGGRAGRDVEQAARSELWLQTWHPTHALYGALRRHDFE
                     AFAASQLKERESAGLPPFSHLAILRADARTPQAARAFLQAAATLGSTLPNAAQVMVYP
                     PVPMAVARIADVERMQMLVESASRMALQRFLAQWLPALHGLRREQQNVQQRILRWAVD
                     VDPSAI"
     gene            complement(4001..4726)
                     /locus_tag="VNU48_04525"
     CDS             complement(4001..4726)
                     /locus_tag="VNU48_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HWI10572.1"
                     /translation="MPGQPPKSLPRATQPLSERELDELQALVDRVPAPLEALDVSMID
                     GYLCGVLLQPRRVAVARWLPHVTDADGRALPPTFDATRLHALVLRRHAELDDAIERRH
                     WFDPWVFELEDDDVRADRPVFDDDDDDDVAGQDVGAIDAVYPWVAGFATALELFPDLM
                     ALDAKALTEPLALIYRHLAPDDLEDADDLLAEIDSLEPPEDLSDAVEGLVRATLLLAD
                     VSRPLAAPGPSRPAARRPRPSNR"
     gene            5035..>5699
                     /locus_tag="VNU48_04530"
     CDS             5035..>5699
                     /locus_tag="VNU48_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWI10573.1"
                     /translation="MGAPEAFTATTEVGHFIDGRTVAATSGRRQPVWNPSTGVVARQV
                     ALASVDEVATAVASAKAAFPAWADTPPIRRARIMNNFLGLLNQHRDTLAAMITAEHGK
                     VFTDAQGEVTRGIDIVEFACGVPQLLKGDFTDQVSTGIDNWTLRQPLGVVAGITPFNF
                     PCMVPCWMFPLAIACGNSFILKPSERDPSAALFMAQLLEQAGLPPGVFNVVQGDKVAV
                     DTLL"
BASE COUNT          861 a         2004 c         1986 g          848 t
ORIGIN      
        1 accagccgcc cgggatctgc gcgagcaact cgttggcgcg cgcgatcgag ccgttcatgc
       61 ccttctcgcg cggggtcagc tcgaaacgcg cgccgtaggc cagcatcagg cggcgccgct
      121 cgatgctcat gctgtcgggc atcaccagga tcagccggta gcccttcacc gcggcgacca
      181 tcgccaggcc gacgccggtg ttgcccgagg tcggctcgat gatcgtgcca ccgggcttga
      241 gcgcgcccga cgcctcggcc gcctcgacca tcgccagcgc gatccggtcc ttgatcgaac
      301 cgcccgggtt ggcgcgctcg ctcttgatgt agacgctgtg cgtgccgccg aacaagcggt
      361 tgatgcggat gtgcggcgtg ttgccgatcg tgtgcaggat gtcgttggcc agcatgaagc
      421 ctccctggag tgcgccgcgc attatgggca tgaccgcacg cagcggtttg cgacaatccg
      481 ccgatgcacg caccgcttcg caacgacagc ttcctgcgcg cctgcctgcg ccagcccacg
      541 actcacacgc cggtctggct gatgcgccag gccggtcgct acttacccga gtaccgcgcc
      601 acgcgcgcga aggcgggcag cttcatgggc ctggcgacga acccggactt cgccaccgaa
      661 gtcacgctgc agccgatcga ccgctacgcg ctcgatgccg ccatcgtgtt cagcgacatc
      721 ctgaccgtgc ctgatgccat ggggctcgga ttcagtctgg atgccggcga agggccgcgc
      781 tttgcgcacc ccgtgcgcga cgaggcggca gtcgcgaagc tcgccgtgcc ggacatgaac
      841 aagctgcgct acgtgttcga tgccgtgacc tcggtccgtc gcgccctcga tggccgcgtg
      901 ccactgatcg gcttctcggg cagcccgtgg accttggcct gctacatggt cgaaggggcc
      961 gggtcgaacg actaccggct cgtcaagacc atgctctaca gccggcccga tctgatgcac
     1021 cgcatcctgg aggtcaacgc cgccgcggtg gcgcagtacc tgaacgcaca ggtcgaggca
     1081 ggtgcacagg ccgtgatgat cttcgacagc tggggcggcg tgctggccga cggcgcgttc
     1141 cagcagttca gcctggccta cacgcgccgg gtgctggccc aactcaagcg cgaacacgag
     1201 ggccagcgga ttccgcagat cgtgttcacg aagggcggcg gcccgtggct ggaggagatc
     1261 gccgcggcgc agccggacgt ggtcgggctc gactggaccg tcaacctcgg cgcggcccgc
     1321 gcgcgcatcg gcgaccgggt cgcgctgcag ggcaacatcg acccgaacgt gctgttcgcc
     1381 gaagcggcgc agatccgggc cgaggtggcg cgcacgctca caagcttcgg cgcaggcccc
     1441 ggccacatct tcaacctcgg ccacggcatc agccagcaca cgccgccgga gaacgtcgcc
     1501 gtgctggtcg acgcggtgca cgagctgtcg gcgggcccgt gtgcccagcc ctgacgctcg
     1561 cgaagacgac ccgcgcaagc ttttcttcgg caggtaagta agcgctcagg gcttgactta
     1621 tccccaaaag cgagcattcg ctttgcacac tcgccaagct gttgcagcgc agcatgggat
     1681 ctcgactaat ccgcgctaag tggttgattt cattgatttg cttgcaatgc cgtgaaagcg
     1741 ggcaatcttt ggcgcaacaa ggcgcagtca gcgtttccgg gcattccggg ggtggttacg
     1801 cacaaagtta tccacagcgg aagtggaaag ctttgaaagg acttaaaaat catcgactta
     1861 acgcagcttc atgaacgaga gccgaggttt gggcgctaac ttcacaagcg agccgacggc
     1921 ggtcggtccc gcgaacgcga cgctggtgcg ggtggccgtc gatgcgccgc agcacaccgg
     1981 gttgtccgcc ccgctcgact acacgagtga gcgcccgctg ccggccggaa ccctcgtcag
     2041 cgttcccctc ggtcggcgcg aagtgccggg cgtggtgtgg cacggtgcgc ccgatgccgc
     2101 cacgaccgtg acgctgcggc cggtgcgcgc ggcgctgacc tcgctgccgc cgctcggcgc
     2161 ggcctggctc gagctggtcg acttcgccgc cgcgtactac cagcgcagca ccggcgagat
     2221 cgcgctgtcg gtgctgccgc ccgaactgcg caagctcgac gacgcgcagc tcgcgaaccg
     2281 catcaagaag ctgcacaagg cgctcggggc gccggccgac gccgcgcggc cgacggcggc
     2341 gccggcgttg accgatgatc aggcgcgcgc gctcgccacg atcgccgccg ccggcacgca
     2401 gcccatgccc gcgccgctgc tgctgcacgg cgtcaccggc agcggcaaga ccgaggtcta
     2461 cctgggtgcc gcagcgcagg cgctcgccgc gggccggcag gcgctggtgc tggtgcccga
     2521 gatcaacctg acgccgcagc tcgaagcgcg tttcggcgaa cgcttcgccg gccggcacat
     2581 cgtctcgctg cacagcgcgc tgacgccggc gcagcggctg cgcagctggc tcgccgcgca
     2641 cctggggctg gccgacctgg tgctgggcac gcggctcgcg gtgttcgcgt cgatgccgcg
     2701 gctcgggctg atcgtggtcg acgaagagca cgacccgtcg tacaagcagc aggagggcgc
     2761 gcgctactcg gcgcgcgacc tggcggtcta ccgcggccgg ctggagcggg cctgcgtcgt
     2821 gctcggctcg gcgacgccgt cgctggagag ctggcaacgc gccgccgagg gccgctacac
     2881 gcggctggcg ctgccgtcgc gtatcggcgg cggcgcgctg ccggcggtgc gcctggtcga
     2941 catgaaccag cagccgaaga cccggggccc ggccaccgcg ctgtcggcac cgctggtggc
     3001 cgcactgcag gagcggctgg cacgcggcga gcagagcctg gtgttcctga accggcgcgg
     3061 ctatgcgccg gtgctgcact gcggcgagtg cggctggaag agcggctgcc cgcattgcag
     3121 cgcctggcgc gtgttccaca agctcgaccg cacgctgcgc tgccaccact gcggctacgc
     3181 cgagcgcgtg ccgcgcgcct gcccggattg cggcaacccg gacatcgcgc cgatcggccg
     3241 cggcaccgag cgcttggagg agcagctcgg cgccctgctg cgcacgcccg acgaccgccc
     3301 ggcacgcatc gcccgcatcg acgccgacag cacgcgcctg aagggcgcgc tggaagcgca
     3361 gctcggcgcg gtccacgccg gcgatgtcga cgtgctggtg ggcacccaga tggtgaccaa
     3421 gggacacgac ttccgccgca tcacgctggt cgccgcggtg aacccggaca gctcgctgtt
     3481 cagcagcgac ttccgcgccc ccgagcgcct gttcgcgctg ctgatgcagg ccggcggccg
     3541 cgccgggcgc gatgtcgagc aagccgcgcg cagcgagctg tggctgcaga cctggcaccc
     3601 gacgcacgcg ctgtacggcg cgctgcgccg ccacgacttc gaggccttcg cggcgagcca
     3661 gctgaaggag cgcgaaagcg ccgggctgcc gccgttctcg cacctcgcga tcctgcgcgc
     3721 cgatgcacgc acgccgcagg cggcgcgcgc attcctgcag gccgccgcga cgctcgggtc
     3781 gaccctgccg aacgccgcgc aggtgatggt ctacccgccg gtgccgatgg ccgtggcgcg
     3841 catcgccgac gtggagcgca tgcagatgct ggtcgagtcg gcctcgcgca tggccttgca
     3901 gcgctttctc gcgcagtggt tgcctgcgct gcatggcttg cggcgcgagc agcagaacgt
     3961 gcagcagcgc atcctgcgct gggccgtcga cgtcgacccg tcagcgattt gaagggcggg
     4021 ggcggcgcgc ggccgggcgg ctcgggccgg gcgcagcgag gggccgggag acgtcggcca
     4081 gcagcagggt ggcgcgcacc aggccctcga ccgcgtcgct gaggtcttcg ggcggctcca
     4141 ggctgtcgat ctcggcgagc aggtcgtcgg cgtcttcgag atcgtcgggt gcaaggtggc
     4201 gatagatcag tgcgagcggc tcggtcagtg ccttcgcgtc gagcgccatc agatcgggaa
     4261 agagctcgag cgcggtcgcg aagccggcga cccaagggta gacggcgtcg atcgcgccga
     4321 catcctgccc ggcgacgtca tcgtcatcgt cgtcgtcgaa gaccggacgg tcggcgcgca
     4381 cgtcgtcgtc ctccagctcg aacacccagg ggtcgaacca atggcggcgc tcgatcgcat
     4441 cgtcgagttc ggcatggcgc cgcaggacca gcgcgtgcag ccgggtcgcg tcgaatgtcg
     4501 gcggcagcgc gcggccgtcg gcgtcggtga cgtgcggcag ccagcgcgcg accgcgacgc
     4561 gccgcggctg cagcagcacg ccgcacaggt agccgtcgat catgctgaca tcgagcgctt
     4621 cgagcggtgc cggcacccga tcgacgaggg cttgcaattc gtcgagttcg cgctcgctca
     4681 gcggctgcgt cgcgcgtggt agcgacttgg ggggttggcc aggcatggcg cattgtcgcg
     4741 cgacgcgttg cgcccttcaa actggggatg gccggcgccc gaggccatcc ctagagtcgg
     4801 ggcactgctg tcacacggcg ccttctgcaa aggcacgaag caaggttcga caggcccgac
     4861 gaagagcatc acggcgaaca gggcgcagaa ccgctaccga tcccaacgac gtcctttccg
     4921 aaggacttcc aaacccaccc gctcgcgcgg gtgggttttt tttggtccgg gtgaccggat
     4981 cacgctggca tagactgcct ggctgatccg ttttccgatg cgaggagatc caccatgggc
     5041 gcacccgaag ccttcacggc cacgaccgag gtcggtcact tcatcgacgg ccgcacggtc
     5101 gcggccacgt ccgggcgccg ccagccggtg tggaacccgt cgaccggcgt ggtcgcacgc
     5161 caggtcgcct tggcctcggt cgacgaggtc gcgaccgctg tcgcgtcggc gaaggccgcg
     5221 ttcccggcct gggccgacac accaccgatc cgccgcgcac gcatcatgaa caacttcctc
     5281 gggctgctga accagcaccg cgacacgctg gccgcgatga tcaccgccga gcacggcaag
     5341 gtcttcaccg acgcgcaggg cgaggtcacg cgcggcatcg acatcgtcga gttcgcctgc
     5401 ggcgtgccgc agctgctcaa gggcgacttc accgaccagg tcagcaccgg catcgacaac
     5461 tggacgctgc gccagccgct gggcgtcgtg gccggcatca ccccgttcaa ctttccctgc
     5521 atggtgccgt gctggatgtt cccgctcgcg atcgcctgcg gcaacagctt catcctcaag
     5581 cccagcgagc gcgacccgtc cgccgcgctg ttcatggcgc aactgctcga gcaggccggc
     5641 ttgccgccgg gtgtcttcaa cgtcgtgcaa ggcgacaagg tcgcggtcga caccttgct
//
LOCUS       DATMJB010000204         2534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_192782, whole genome shotgun sequence.
ACCESSION   DATMJB010000204 DATMJB010000000
VERSION     DATMJB010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192782"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="VNU48_04535"
     CDS             complement(<1..1374)
                     /locus_tag="VNU48_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HWI10574.1"
                     /translation="MPTPANPTALAQQARRSYVEGLLAGLPAVVQAIDQGARVLLGKV
                     AEPAVTGRRRDAVAELHKAAPHWLQGMTSMLRGARQSGLVSATHPGDLPLTASRHAKL
                     SLVDDDTIEHEIISSKLALSMMDRASWEFSDLRSRMNVLEARDELDTNDVLRPHVLAR
                     IVSVSWRAANLSLEAWRELQGVLQDEFASFAEEGYHETNRFLLERHVLPDVDLRPFIR
                     RSRNSATGFGAAPAAATRGAFVQSGGAAAEPRGEVGEETRMMTRAGGLARGADHAEQV
                     LGRLNRLVARQLPGFGVTQQDRPVVTAPRLKAAINDVQQGIARRVATSEHAATVAGRV
                     SAPALLEELNQRKQALKQAASTPVERATIEIVALLFQSILTEERIPTSVRVWFARLQM
                     PVLRVAVSEPDFFATIDHPARRLIDRMGSCVMGFDANSRAVGDILEKEIKRVVQVVEA
                     YPDTGRRV"
     gene            complement(1415..2449)
                     /gene="secF"
                     /locus_tag="VNU48_04540"
     CDS             complement(1415..2449)
                     /gene="secF"
                     /locus_tag="VNU48_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016723719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HWI10575.1"
                     /translation="MEFFRIRRDLPLMRHALVFNIISALTFLAAVFFLATRGLHFSIE
                     FTGGINQQVSYAQTADVEKIRAGVEKLNFGEVQVQNFGSSRDVLIRLPAKQGTKQTEV
                     AATVFDTLCKAENGSVSVRQSVTDKGETVSRPVCSAGTTEPVQQRSVEYVGPQVGSEL
                     AINGAYALAAVVIGIVIYLAIRFELKFAIAAIIANLHDVVIILGFFAFFQWEFNLTVL
                     AAVLAVLGYSVNESVVIFDRIREAFRRFRKMNTHEVIDHAITSTMSRTIITHGCTQAM
                     VLSMLIFGGPTLHGFAIALTIGILFGIYSSVFVAAAIAMWLGVRREDLVKVGTAKSTD
                     PDDPNAGAVV"
BASE COUNT          396 a          876 c          847 g          415 t
ORIGIN      
        1 cacgcgccgt ccggtgtcgg ggtaggcctc gacgacctgc acgacgcgct tgatctcttt
       61 ctcgaggatg tcgccgaccg cgcggctgtt cgcgtcgaag cccatcacgc acgagcccat
      121 gcggtcgatc aggcggcgcg ccgggtgatc gatggtcgcg aagaagtcgg gctcactgac
      181 cgccacgcgc agcaccggca tctgcagccg cgcgaaccag acgcgcaccg aggtcggaat
      241 gcgctcttcg gtcaggatgc tctggaacag cagcgcgacg atctcgatcg tcgcgcgctc
      301 gaccggcgtg cttgcggcct gcttcagggc ctgcttgcgc tggttcagct cttccagcag
      361 cgccggcgca ctgacacggc cggcgacggt ggcggcatgc tccgaggtcg cgacgcgccg
      421 cgcgattccc tgctgcacat cgttgatcgc ggccttcagg cgcggggcgg tcacgaccgg
      481 gcggtcctgc tgcgtgacgc cgaaccccgg cagctggcgt gcgacgagcc ggttcagccg
      541 gcccagcacc tgctcggcgt ggtcggcgcc gcgcgccaga ccgccggccc gggtcatcat
      601 gcgcgtctct tcgccgacct cgccacgcgg ctcggcggcc gcgccacccg actgcacgaa
      661 cgcgccacgc gtcgctgcag ccggagcggc gccgaagccg gtcgcgctgt tgcgcgagcg
      721 ccggatgaac ggccgcaggt cgacgtcggg cagcacgtgg cgctcgagca ggaagcgatt
      781 cgtctcgtgg tagccctctt cggcgaagct cgcgaactca tcctgcaaca cgccctgcag
      841 ttcgcgccac gcctccaggc tcaggttggc ggcacgccac gatacgctga cgatgcgcgc
      901 cagcacgtgc ggacgcagca cgtcgttggt gtcgagttcg tcgcgggctt cgagcacgtt
      961 catgcgcgag cgcagatcgg agaactccca cgacgcgcgg tccatcatcg acagcgcgag
     1021 cttggagctg atgatctcgt gctcgatggt gtcgtcgtcc accaggctga gcttcgcgtg
     1081 gcgactcgcc gtcagcggca gatcgccggg gtgcgtggcc gacaccagcc cgctctggcg
     1141 cgcaccgcgc agcatcgagg tcatgccctg cagccagtgc ggcgcggcct tgtgcagttc
     1201 ggccaccgcg tcgcgtcggc gcccggtcac tgccggctcg gcgaccttgc cgagcaacac
     1261 gcgcgcacct tgatcgatcg cctgcaccac cgccggcagg ccggcgagca acccctcaac
     1321 gtacgagcgc ctggcctgct gggccaatgc cgtcgggttg gcgggggtcg gcatggggag
     1381 tgacgctacc tggggaggga actacgctgc gactctacac cacggccccg gcattcgggt
     1441 cgtccggatc ggtgctcttg gccgtgccga ccttgaccag atcctcgcgc ctgaccccca
     1501 gccacatcgc gatcgccgcg gcgacgaaca ccgacgaata gatgccgaac aggatgccga
     1561 tcgtcagcgc gatcgcgaag ccgtgcaagg tcggcccgcc gaagatcagc atcgacagca
     1621 ccatcgcctg cgtgcagccg tgcgtgatga tcgtgcggct catcgtgctg gtgatcgcgt
     1681 ggtcgatgac ctcgtgcgtg ttcatcttgc gaaagcgccg gaaggcttcg cggatccggt
     1741 cgaagatcac caccgactcg ttgaccgaat agcccagcac cgcgagcacg gcggcgagca
     1801 cggtcaggtt gaactcccac tggaagaacg cgaagaagcc caggatgatg acgacgtcgt
     1861 gcaggttcgc gatgatcgcc gcgatcgcga acttcaattc gaagcggatc gccaggtaga
     1921 tcacgatgcc gatgacgacc gccgccagcg cgtaggcgcc gttgatggcg agttcgctgc
     1981 ccacctgcgg cccgacgtac tcgacgctgc gctgctgcac cggctcggtc gtgcccgccg
     2041 aacacaccgg ccggctcacc gtctcgccct tgtcggtgac ggactggcgc acgctgacgc
     2101 tgccgttctc ggccttgcac agcgtgtcga acacggtggc cgcgacttcg gtctgcttcg
     2161 tgccctgctt ggccggcagg cggatcagca cgtcgcgcga gctgccgaag ttctgcacct
     2221 gcacttctcc gaagttcagc ttctcgacgc cggcgcggat cttctcgaca tcggccgtct
     2281 gcgcatagct cacctgctgg ttgatgccgc cggtgaactc gatcgagaaa tgcagcccgc
     2341 gcgtcgcgag gaagaacacc gcggcgagga aggtgagcgc cgagatgatg ttgaacacga
     2401 gcgcatgccg catcagcggc aggtcgcggc ggattcgaaa gaattccatc tgagtttccc
     2461 agtcggcccg gtatcaggtc ttggccggaa cgttcgtgtt cgggttcgcg cttgtgcccg
     2521 cgtccggctt ccag
//
LOCUS       DATMJB010000205         2074 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_843908, whole genome shotgun sequence.
ACCESSION   DATMJB010000205 DATMJB010000000
VERSION     DATMJB010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843908"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VNU48_04545"
     CDS             <1..438
                     /locus_tag="VNU48_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HWI10576.1"
                     /translation="HSTPYLTRDFFARMARTMPENWLLFIASRGGKRIAASLIVIDPA
                     RRSAFGRYWGAVEHVPLLHFEACYYQPLQWCIANGYTRFEGGAQGEHKMARGLLPVQT
                     WSAHWLAHPQFARAVGDFLEREGAGVAGYLDELNERRPFKAGA"
     gene            complement(449..>2074)
                     /locus_tag="VNU48_04550"
     CDS             complement(449..>2074)
                     /locus_tag="VNU48_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HWI10577.1"
                     /translation="EPITADELARAKLKWLKDWDQSFTNPETVGLAMSESISQGDWRL
                     FFLTRDRVRDATLADVQRVAAQYLLPANRTLGTYLPTEQPARAPALARVDLAQTMKEF
                     KPQAAAAKVEAFEATPANIDARTQTFAVGGVKGALLPKGTRGQAVSAVLMLQFGDEKS
                     LFGQNAAAQAAAALLDKGTKTLNRQQVQDRLDALKTELRVSASAGRVTIALQSRRDEL
                     PAAIELVGDLLRNPSFPADALDEFKRSALSSIEQQRKEPGALARNTLDRLGDPYPRGD
                     LRYHASFDELADDYRALTIEQVRGFHSRFYGAGSGEFSAVGDLDAAKVRAALQTALTG
                     WSAGAPFTRVPQPLLAVKPQQLMIATPDKPNANMLVRLAVPLNDLDADYPALTMANFL
                     LGSGGSARLWKRIREGEGLSYDVRSVIDWNNIDRNSLWQASAIFAPQNRAKVETAFRE
                     EVARALKDGFTAQELAEGKSSLLNFRQLSRAQDAGLANALANNLYLGRTFALAAQVDA
                     AIGQLTLEQVNAALRKYVTPEQFVSAFAGDFKP"
BASE COUNT          293 a          730 c          712 g          339 t
ORIGIN      
        1 cactcgacac cgtacctgac gcgcgatttc ttcgcacgca tggcgcgcac gatgcccgag
       61 aactggctcc ttttcatcgc atcgcgcggc ggcaagcgca tcgccgcgtc gctgatcgtg
      121 atcgacccgg cgcggcgcag cgcattcggc cgctactggg gcgcggtcga gcatgttccg
      181 ctgctgcact tcgaggcgtg ttactaccag ccgctgcagt ggtgcatcgc gaacggctac
      241 acgcgcttcg aaggcggcgc gcagggcgag cacaagatgg cgcgcggcct gctgccggtg
      301 cagacctggt cggcgcactg gctcgcgcac ccacagttcg caagggcggt cggcgacttc
      361 ctcgaacgcg aaggcgcggg cgtcgccggc tacctcgacg aactcaacga gcgccggccg
      421 ttcaaggccg gcgcttgaag cagccaggtc acggtttgaa atcgccggcg aatgccgaga
      481 cgaactgctc gggcgtcacg tatttgcgca acgcggcatt gacctgctcc agcgtcagct
      541 gaccgatcgc cgcatcgacc tgcgcggcca gcgcgaacgt gcgtcccagg tacaggttgt
      601 tggccagcgc attcgccagg ccggcgtcct gcgcgcgcga gagctggcgg aagttcagca
      661 ggctcgactt gccttcggcc agctcctgcg cggtgaagcc gtccttcagt gcgcgggcga
      721 cctcttcgcg gaacgcggtc tcgaccttgg cgcggttctg cggcgcaaag atcgcactcg
      781 cctgccacag cgagttgcgg tcgatgttgt tccagtcgat cacgctgcgc acgtcgtacg
      841 acaggccctc gccctcgcgg atgcgcttcc acagtcgcgc actgccgccc gagccgagca
      901 ggaagttcgc catcgtcagc gccgggtagt cggcgtcgag gtcgttcagc ggcacggcca
      961 ggcgcaccag catgttcgcg ttcggcttgt cgggcgtggc gatcatcaac tgctgcggct
     1021 tcaccgcgag cagcggctgc ggcacgcgcg tgaaaggcgc accggcgctc cagcccgtga
     1081 gcgcggtctg cagtgcggcg cgcaccttgg cggcgtccag gtcgccgacg gccgagaact
     1141 cgccgctgcc cgcgccgtag aaacgactgt gaaaaccacg cacctgctcg atcgtcagcg
     1201 cgcggtagtc gtcggccagc tcgtcgaagc tcgcgtggta gcgcaggtcg ccacgcgggt
     1261 acgggtcacc caggcggtcc agcgtgttgc gcgccagcgc gcccggctcc ttgcgctgct
     1321 gctcgatgct gctcagcgca ctgcgcttga actcgtcgag cgcatccgcc gggaagctcg
     1381 ggttgcgcag cagatcgccg acgagctcga tcgcggcggg caactcatcg cggcgcgact
     1441 gcagcgcgat cgtcacgcgg cccgccgagg cgctgacgcg cagctcggtc ttgagtgcgt
     1501 cgagccggtc ctgcacctgc tggcggttca gcgtcttcgt gcccttgtcg agcagcgcgg
     1561 cagcggcctg cgccgcggcg ttctggccga acaggctttt ctcgtcgccg aactgcagca
     1621 tcagcaccgc gctcaccgcc tggccgcgcg tgcccttggg cagcagcgcg cccttcacgc
     1681 cgccgaccgc gaaggtctgg gtgcgcgcat cgatgttggc cggcgtggcc tcgaaggcct
     1741 cgaccttcgc cgccgccgcc tgcggcttga actccttcat cgtctgcgcg aggtcgacgc
     1801 gcgccagcgc cggcgcgcgc gccggttgct cggtcggcag gtaggtgccg agcgtgcggt
     1861 tcgcgggcag caggtattgc gcggcgacgc gctgcacatc ggccagcgtc gcgtcgcgaa
     1921 cgcggtcgcg ggtcaggaag aacagccgcc agtcgccctg cgagatcgac tccgacatcg
     1981 ccaggcccac cgtctcgggg ttggtgaacg actgatccca gtccttcagc cacttgagct
     2041 tggcgcgcgc gagctcgtcg gccgtgatcg gctc
//
LOCUS       DATMJB010000206         6262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_729507, whole genome shotgun sequence.
ACCESSION   DATMJB010000206 DATMJB010000000
VERSION     DATMJB010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729507"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..1921)
                     /locus_tag="VNU48_04555"
     CDS             complement(164..1921)
                     /locus_tag="VNU48_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWI10578.1"
                     /translation="MNNLKVSTRLIMLIGALSLLLVGIGGLGLYGISQCSEALRSVYE
                     GSTVPTAKLGEIRSMVLHNRLAINIALVTPTAEVIKERTAEIESNIATITKIWDAYVA
                     ADLTTEEEKLAKTVASDRKRFVQEGLKPAVVALRANDLKEAQRLVVEKIRPLSIPFEA
                     GNDALIKLQIDEAQKEYMAAAARYAVIRVVAIASIAAGLMFAGLFGFVLVRSIGRQLG
                     AEPGEAADLARSVAAGDLSVRIDLRQGDTTSLMAQLKEMQTSLAKVVFGVRQNSESVA
                     TASVQIAQGNLDLSQRTEEQASALEETAASMEQLGSTVKQNADNAKQANQLALGASTV
                     AIKGGEVVGQVVDTMKGINDSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVASEVRNLAGRSAEAAKEIKSLINASVERVEQGTALVDQAGVTMTEVVSSIKRV
                     TDIMGEISAASIEQSAGVSQVGEAVSQMDQVTQQNAALVEESAAAAESLKVQAQQLVQ
                     AVAVFKLAHSAHAVEAAPAGEARNVAERRSPDRAKNVTRPAFGKAKPAVAEATAPPQL
                     EPTLAPAAPRSGTDDWETF"
     gene            2210..2896
                     /locus_tag="VNU48_04560"
     CDS             2210..2896
                     /locus_tag="VNU48_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI10579.1"
                     /translation="MLPKTLALIDDDQDYAEFLALYLREQGVQVRVYADSNDLLADAE
                     AHRYEFYVVDLMLPGVDGVELIKILRRRSNAGLLVVSGRLAPDVFKTVVAAGADMYLT
                     KPVQFEQVVVAIGAVQRRAAGAAQAEAAWRLDRLARELVAPDGTRVDLSAGDLVVLEC
                     FVEAQGETVTRESLRLRLGRSAPDGDVDGLNAAIYRLRRRIERATPALVPLQSKSRVG
                     YVFRAPLALA"
     gene            complement(2924..4783)
                     /locus_tag="VNU48_04565"
     CDS             complement(2924..4783)
                     /locus_tag="VNU48_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI10580.1"
                     /translation="MMSPLIDAQAYFFVQGLLFIALPLTAWTILSGRHDGATVAQWCV
                     SGLMAGAGFVLIGLRGRVPDVLGYPVATALAYGAYLLRGGALQRELGQRPRWALLTLI
                     CALATVGFQVSMNLGRDPRELYSAGICLAGASWLACLAFTLARREKTRSGVLLGAVFA
                     LSAVASTIRAAAVLDGWGEGRAPTALDYGLVNVAALLTALYANLGYVGLVLERSRAKE
                     QARKEDHEREHALRLQAEQHAMELDALRAERRDKLNESTPAMLHSIDPQGHLLAVSDT
                     WLSKLGYTRAEVIGRPSADFLTPPSREHARQVVLPEFFRMGRCDNVAYQMLRKDGSVL
                     DVLLSAVLERDALGRPLRSWAVIEDVTEKLVREAELAREHALRLLVEQHAHKLDALLA
                     ERSEMLHVLAHEVRQPLQNASAALQSAASALGQTREPVAADRLWRAQAVLGEVLAGLN
                     NTLAAAALLAGSSAATSEDVDIDAMIEIALGDIAQEDRARVQIEHATTTRTAAMDPGL
                     MRLALRNLLDNALSYSPRGSPVVLRVSDSDRPLALIVDIMDGGAGIEADLRPRLFQRG
                     ERGALGAGRPGSGLGLYIVRRVAEMHSGRIDAVPRLGGGTVMRLTIPQGLPPE"
     gene            5203..5352
                     /locus_tag="VNU48_04570"
     CDS             5203..5352
                     /locus_tag="VNU48_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10581.1"
                     /translation="MTTAIATIPLSAVRATASRVKSRAQRAGLIAQLIGSTRLIDKLE
                     PSAAE"
     gene            5349..>6262
                     /locus_tag="VNU48_04575"
     CDS             5349..>6262
                     /locus_tag="VNU48_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWI10582.1"
                     /translation="MTQAGSGNAAWQRRSLQHVWHPCTQMQRAMQVPPLPIARGQGPW
                     LEDYEGRRYFDATSSWWVNLFGHADERINAALKDQLDRLPHVMLAGCTHAPAVELAER
                     LAALTGGVLGHCFFASDGASAVEIALKMSFHCWRNLGLGAKREFVCLRQGYHGETLGA
                     LAVTDVAVFRDAYDPLLMRSHSVMSPDARQALPGESPADVATRALVDLRRLLGAKHAQ
                     IAAVIVEPLVQCAAGMAMHDPGYLRALRALCDEYAVHLIADEIAVGCGRTGSFFAFEQ
                     AALPGRAALWPDFVCLSKGISGGYLPLSL"
BASE COUNT          961 a         2119 c         2080 g         1102 t
ORIGIN      
        1 cgggctagct tcacatttcg cggctcgctc ggtcctgagc tcgtgggtca cgagcagctc
       61 attggctacg tccagttcgt caccggtgca ctcgatcgct acacgtcaga cggactccat
      121 cgcgggcgac gttcatgcca gaacctcggc ggaggtttcc tgttcagaaa gtttcccagt
      181 cgtcggtgcc gctgcgcggg gccgccggtg caagggtcgg ttcgagctgc gggggtgccg
      241 tggcctcggc cacggccggc ttggccttgc cgaatgcggg tcgcgtcacg ttcttcgcgc
      301 ggtccgggct ccggcgctcg gccacgttcc gtgcttcccc cgcaggggcg gcctcgacgg
      361 catgcgcgct gtgcgccagc ttgaagaccg ccacggcctg caccagttgc tgcgcctgca
      421 ccttcaggct ctcggccgcg gcggcgctct cttccaccag cgcagcgttc tgttgcgtta
      481 cctggtccat ttggctcacg gcctctccca cttgcgaaac ccccgcgctc tgctcgatgc
      541 tcgcggcgct gatctcgccc atgatgtcgg tgacgcgctt gatcgagctg accacctcgg
      601 tcatcgtgac accggcctga tcgaccaatg ccgtgccttg ctcgacgcgt tcaacgctgg
      661 cgttgatcag gctcttgatc tccttggctg cttcggccga acggcccgcc aggttgcgca
      721 cctcactggc caccaccgcg aagccgcgac cctgttcgcc ggcacgcgcc gcttccaccg
      781 ccgcgttcaa cgccaggatg ttggtctgga acgcgatgcc atcgatgacg ctgatgatgt
      841 cggcgatctt cttcgagctg tcgttgatgc ccttcatggt gtccaccacc tggccgacca
      901 cctcgccgcc cttgatggcc accgtgctgg cgcccagcgc gagctgattg gcctgcttgg
      961 cgttgtcggc gttttgcttg acggtcgagc ccaactgctc catcgacgcg gcggtctctt
     1021 cgagagccga ggcctgctcc tcggtgcgct ggctcaggtc cagattgcct tgggcgatct
     1081 ggaccgatgc ggtggccacc gactcggagt tctggcgcac gccgaacacc accttggcca
     1141 ggctcgtctg catctccttg agctgcgcca tcaggctggt ggtgtcgcct tgtctgagat
     1201 cgatgcgcac gctgaggtcg ccggcggcca cgcttcgtgc caggtcggcg gcctcaccgg
     1261 gctcggcacc gagctggcga ccgatggagc gcaccaatac aaagccgaac agccctgcga
     1321 acatcagacc cgcggcgatg gaagcgatcg cgaccacgcg gatcacggca tagcgcgcgg
     1381 ccgcggccat gtactccttc tgggcttcgt cgatctgtag tttgatgagt gcgtcgttgc
     1441 ccgcttcaaa cgggatggac agcggacgga tcttttccac gaccaggcgc tgggcctctt
     1501 tcaggtcgtt ggcacgaagc gcgaccacgg ccggcttaag cccttcctga acgaacctct
     1561 tgcggtcgct ggcgacggtc ttggcaagct tttcctcctc agtcgtcaga tcggcggcga
     1621 cgtaggcgtc ccagatcttg gtgatcgtgg cgatgttgga ttcgatttcc gcggtgcgtt
     1681 ctttgatgac ttcggcggtc ggggtcacca gcgcgatgtt gatggccagc cggttgtgca
     1741 gcaccatgga cctgatctcg ccgagcttcg cagtgggcac tgtgctgcct tcgtagaccg
     1801 atctcagcgc ctcgctgcac tggctgatgc cgtacaggcc caggccgccg atgccgacca
     1861 gcagcagtga cagcgcgccg atcagcatga tgagccgggt ggaaactttc aagttgttca
     1921 tgatgaatgt ccttcgcact gcagcgagat ggtctagttc agggttcgct cgaccagccc
     1981 catctcggcg ccggtcggag tgctcgggcg ctgggaaggc gcctgtcgag cgtctgtttg
     2041 acatggacca acgcaatatc gggcgaataa atcatcgctt gaggaatttg tacgtcttcg
     2101 ccaaaattgg cgcggctttg aggcgcttat cgagcggctt gaccggtcgc gatatgggcg
     2161 accatcaaag cttccaactc cgtgacgcac cgccattcct gaagctgcca tgctgcccaa
     2221 gaccctcgcc ctgatcgatg acgatcagga ctacgccgag tttctggcgc tgtacttgcg
     2281 cgagcagggc gtgcaggtgc gcgtctacgc ggacagcaat gatttgctgg ccgatgccga
     2341 agcgcaccgc tacgagttct atgtcgtcga tctaatgctg ccgggtgtcg acggcgtcga
     2401 gctgatcaaa atcctgcgcc gacgcagcaa cgctgggttg ctggtggtct ctggacgctt
     2461 ggcgccggat gtattcaaga ccgtggtcgc cgccggtgcc gacatgtatc tgaccaagcc
     2521 ggtgcagttc gagcaggtgg tggtggccat cggcgcggtc cagcggcgcg ccgccggcgc
     2581 ggcgcaggcc gaagccgcgt ggcggctgga tcggctggcg cgagaactgg tggcccccga
     2641 cggtacgcgt gtggacctca gtgccggcga cttggtcgtc ttggaatgct ttgtcgaggc
     2701 gcagggcgag accgtgacgc gcgagtcctt acgcctgcgc ttgggtcgaa gcgcccctga
     2761 cggcgacgtc gacggactga acgccgccat ctaccggctg cgacgccgca tcgagcgggc
     2821 gacgccggcg ctcgttccgc tgcagtccaa gtcccgtgtc ggctacgtat ttcgggcgcc
     2881 cttggcactg gcctgatcga tgtgccgcgc aacggatgac gcgtcattca ggtggcagtc
     2941 cttgcgggat cgtcaagcgc atcacggtgc cgccaccgag tcggggaacc gcgtcgatcc
     3001 ggccgctgtg catctcggcc acgcggcgca cgatgtacag gcccaggccg ctgccgggac
     3061 ggcccgcacc cagagcccca cgctcgccac gctggaacag acgcggccga agatcggcct
     3121 cgatgcccgc gccgccatcc atgatgtcca cgatcagggc aagcggtcgg tccgagtccg
     3181 acacgcgcaa gacgaccgga gatcctcgag gcgagtagct cagtgcgttg tcgagcaggt
     3241 tcctcaaggc caggcgcatc aggccagggt ccatggcggc ggtgcgcgtc gtcgtggcgt
     3301 gttcgatctg gacgcgcgcg cgatcctcct gggcgatgtc accgagggcg atttcgatca
     3361 ttgcgtcgat atcgacgtct tccgacgtgg cggcactcga gccggccagc agcgctgcgg
     3421 cggcgagcgt gttgttcagc cctgcgagca cctcgcccag cacggcttgc gcccgccaga
     3481 gccgatcggc agcaaccggc tctcgggtct gccccaacgc ggacgccgcg ctctgcaaag
     3541 cggccgacgc attctgcaac ggttggcgca cttcgtgtgc caacacatgc agcatctcgc
     3601 tgcgctcggc cagcaacgca tcgagtttgt gcgcgtgctg ttccactaga aggcgcaagg
     3661 cgtgttctcg ggcaagttcg gcttcgcgca cgagcttctc ggtcacgtcc tcgatcacgg
     3721 cccacgagcg caaggggcgt cccagggcgt cacgctccag cacagctgag agcagtacat
     3781 cgagcacgct gccgtccttg cgcagcatct ggtaggcgac gttgtcgcaa cggcccatgc
     3841 ggaaaaactc cggtagcaca acctgacgcg catgctcgcg cgagggcggc gtcaggaagt
     3901 cggccgaagg cctgccgatg acctccgcac gcgtgtagcc caacttggac agccaggtat
     3961 cgctgacggc gagcaggtgc ccctgtggat cgatggagtg cagcatggcc ggggtcgact
     4021 cgttgagctt gtcccgacgc tctgcacgca gggcgtccag ctccatggca tgctgctcgg
     4081 cctgcaggcg cagcgcgtgc tcgcgctcgt ggtcttcctt tctcgcttgc tccttggcgc
     4141 gcgagcgttc cagcaccagg ccgacgtagc ccaggttggc gtacagagcc gtcagcaggg
     4201 ccgcgacgtt gaccaggcca tagtccaggg ccgtgggggc gcgtccttcg ccccagccgt
     4261 ccagcacggc cgcagcgcgt atcgtcgacg cgaccgcgga caatgcaaac acagcgccca
     4321 gcaggacgcc actgcgcgtc ttttcgcggc gggccagagt gaacgcgagg caggccagcc
     4381 acgacgctcc agctaggcag atgccggcgc tgtagagctc gcgcgggtca cgtcccaggt
     4441 tcatcgacac ctggaatccc acggtcgcta gcgcgcagat gagcgtcagc agcgcccagc
     4501 ggggccgctg ccccagctcg cgctgcaggg cgccgccgcg caacagatag gcgccgtagg
     4561 cgagggccgt ggcaacgggg tagcccagta cgtcggggac gcgcccgcgc aggccgatca
     4621 gtacgaatcc ggcacccgcc atcaagccgc tgacgcacca ctgcgccacc gtggccccgt
     4681 cgtggcggcc ggacaggatg gtccaagcgg tcaggggcag ggcgatgaac agcagtccct
     4741 ggacgaagaa ataggcctgt gcgtcgatga ggggggacat catggtcttg tgatgcgagg
     4801 cgagtgcgca attgtcgcgg cgagagtgac gggaccacgc tcgacatctt cggcgcgctg
     4861 ctcaacgtcg acccgagcgc gaggaccttc aacctgtccg agggaccgta tcgcacgtac
     4921 acgctgacct gggacgatga gaccgttttc ggcgcgggcc tgtcgggcag cacgcgatct
     4981 gcgccgtgcc ctgcgccgtc gggcacagtg ctgcgatgca gcataatccg gccacttttc
     5041 cgcatcacac ggaccggcgc caccaagcgt tctaccgtgc atggccctgg ggcaggacgt
     5101 ccgtcacccg gaaatcgtcg cccgagtggc caacgaggag acgccggcgc cccgaacggg
     5161 cggtgccggc gtgctaccca acctctggcg caacgagccc gcatgaccac cgcgattgcc
     5221 acgatccccc tgtccgccgt gcgcgccacg gcttcccgcg tcaagagccg ggcgcagcgc
     5281 gccgggctga tcgcgcagct gatcggatcg acgcggctga tcgacaagct cgaaccgagc
     5341 gccgccgaat gacgcaggcc ggttcgggca acgcggcgtg gcagcgccgc agcctgcagc
     5401 acgtgtggca cccctgcacg cagatgcagc gggcgatgca ggtgccgccc ttgccgatcg
     5461 cgcgcggcca gggcccctgg ctggaagact acgagggccg gcgctatttc gacgcgacca
     5521 gctcgtggtg ggtcaacctg ttcggccacg ccgacgaacg catcaacgcc gcgctgaagg
     5581 accagctcga ccgcctgccg cacgtgatgc tggccggctg cacccacgca ccggcggtcg
     5641 aactcgccga gcgcctggcg gcgctgacag ggggcgtgct gggccactgc ttcttcgcca
     5701 gcgatggtgc ctcggccgtc gagatcgcgc tgaagatgag ttttcactgc tggcgcaacc
     5761 tcgggctcgg cgccaagcgc gagttcgtct gcctgcgcca gggctaccac ggcgagaccc
     5821 tgggtgcgct cgcggtcacc gacgtggcgg tgttccgcga cgcgtacgac ccgctgctga
     5881 tgcgttcgca cagcgtcatg tcgccggacg cgcgccaggc gctgccgggc gagtcgcccg
     5941 cggacgtcgc cacgcgtgcc ctcgtcgacc tgcgccgtct gctcggcgcg aagcacgcgc
     6001 agatcgccgc cgtcatcgtc gagccgctgg tgcagtgcgc ggccggcatg gcgatgcacg
     6061 acccgggcta tctgcgcgcg ttgcgtgcgc tctgcgatga gtacgcggtg cacctgatcg
     6121 ccgacgagat cgccgtcggc tgcggccgca ccggcagctt cttcgccttc gagcaggccg
     6181 cgctgcccgg gcgggctgcg ctctggcccg acttcgtctg cctgtccaag ggcatcagcg
     6241 gcggctacct gccgctgtcg ct
//
LOCUS       DATMJB010000207         3919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_29216, whole genome shotgun sequence.
ACCESSION   DATMJB010000207 DATMJB010000000
VERSION     DATMJB010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29216"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..924
                     /locus_tag="VNU48_04580"
     CDS             217..924
                     /locus_tag="VNU48_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWI10583.1"
                     /translation="MTFVFTPPAAAAVPVQGGAATALFPVHRIYCVGRNYVEHAIEMG
                     HSGREAPFFFMKPADAVLPVAEGTVGRMHYPSLSKSLHHELELVVAIGKGGRDIPAAQ
                     ALEHVWGYAVGLDMTRRDLQGEAKQLGRPWCIGKGFDESAPIGPIKRADECRLDADTK
                     IALLVNGRPRQASTLGKLIWSVPEIIEHLSKAWELAPGDLIFSGTPEGVAAVEVGDTL
                     SARVDGVGTLELEIVAR"
     gene            934..1572
                     /gene="maiA"
                     /locus_tag="VNU48_04585"
     CDS             934..1572
                     /gene="maiA"
                     /locus_tag="VNU48_04585"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HWI10584.1"
                     /translation="MQLYNYYRSSASYRVRIALALKGLDCELLPVHLLRHEQESAAYR
                     ALAPSQLVPSLVIDDQLLTQSMAIIEYLDECHPQPSLLPGDALARARIRSLAQDVACE
                     IHPLDNLRVLRYLVHEMKVGEDDKTRWYRHWVESGLEALEQRLAGDARTGRFCHGDTP
                     TLADCVLVPQIVNAQRFDCRLDHLPTVMRVFDECMRLAAFAATQPSAYPGPA"
     gene            1572..2354
                     /gene="pgeF"
                     /locus_tag="VNU48_04590"
     CDS             1572..2354
                     /gene="pgeF"
                     /locus_tag="VNU48_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HWI10585.1"
                     /translation="MRDALLRHPDWLAPAWDVARVGALMTTRHGGVSMAPFDSLNLRA
                     AVGDDPPAVAENQRRLAQAIGARPVFLNQVHGAAVAHLSAADVRAGAALHTADASVTT
                     EPGVGCAVQVADCLPVLFAAPQGRAVGAAHAGWRGLALGVLEATLAAVCAAAQCEPGE
                     VQAWLGAGIGPAHFEVGPDVLEAFGASAAGSPRFVARQPGKWLADLPALARDRLQAAG
                     VRAVSGGDWCTVADASRFFSFRRDRVTGRMAAVVWIEATQGR"
     gene            complement(2271..2546)
                     /locus_tag="VNU48_04595"
     CDS             complement(2271..2546)
                     /locus_tag="VNU48_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10586.1"
                     /translation="MLLIAIAWIYVVLMVAVVEAASPIGTLLGALFTLLLYGVLPLGI
                     VAYLFFSPARRRARRAADARSAALGGLDPHDGGHAPGDAVAPEREES"
     gene            2793..>3919
                     /locus_tag="VNU48_04600"
     CDS             2793..>3919
                     /locus_tag="VNU48_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI10587.1"
                     /translation="MSGLSLPMPAVAELQSDYLKQSTELWNQTLHAQAAAPADRRFAA
                     SDWAKNPASAYTAQMYLLNARTLMQMADCLQGDEKTRGRIRFALQQWIDAAAPSNYLA
                     LNPEAQRKALDTQGESIALGMKHLLDDVRQGHVSQTDESVFEIGRNVATTEGAIVFEN
                     ELFQLIEYKPLTATVFERPMLFVPPCINKYYILDLQPENSVIRYTVAQGHRLFVISWR
                     NADESIKTYTWDQYIEDAAIRAIGLVQEITGSKQINTLGFCVGGTILATALAVLAARD
                     RKPAASMTLLTTFLDFSSTGVLDLFVDETMVRMREMTLGPKSPGGGSLLKGQELATTF
                     SFLRPNDLVWNYVVGNYLKGETPPPFDLLYWNSDGTNLPGP"
BASE COUNT          640 a         1360 c         1358 g          561 t
ORIGIN      
        1 gcaggtggcg cgttcgcggt tgtcgtcggc cggcaccgag tagaccgtcg cgccgccgca
       61 gaccttgcag tgccggatgc cgtgcgggat caacatgggg cccgagtgta gccgcgcgac
      121 ccgcgctcgc atcggcgtcg cgaggcgctg cgtggcatca ttccgcgctg atctcggcca
      181 cggccgcgga ctctttcgac gaaccaggat gcgatcatga ccttcgtttt cacgccgccc
      241 gccgcggccg ccgtcccggt ccagggaggc gcggcgaccg cgctgttccc ggtgcaccgc
      301 atctactgcg tcggccgaaa ttacgtggag cacgcgatcg agatgggcca cagcggccgc
      361 gaggcaccgt tcttcttcat gaagccggcc gatgcggtgc tgccggttgc cgagggcacg
      421 gtcggtcgga tgcactaccc gagcctgagc aagagcctgc accacgaact cgagctcgtc
      481 gtcgcgatcg gcaagggcgg gcgcgacatc cccgcggcac aggcgctcga acacgtctgg
      541 ggctacgcgg tgggcctgga catgacgcgc cgcgacctgc agggcgaagc gaagcagctc
      601 ggccggccgt ggtgcatcgg caagggcttc gacgagtcgg cgccgatcgg cccgatcaag
      661 cgcgccgacg aatgccggct cgacgccgac acgaagatcg cgctgttggt gaacggccgg
      721 ccgcgccagg ccagcacgct gggcaagctg atctggagcg tgccagagat catcgagcac
      781 ctgtccaagg cctgggagct cgcgcccggc gacctgatct tcagcggcac gcccgaaggc
      841 gtcgcggcgg tcgaggtcgg cgacacgctg agcgcgcgtg tcgacggggt cggcacgctc
      901 gagctggaga tcgtcgcgcg ctgaggcacg gccgtgcagc tctacaacta ctaccgctcg
      961 tcggcctcgt accgcgtgcg catcgcgctc gcgctgaagg ggctggactg cgaactgctg
     1021 ccggtgcacc tgctgcgcca cgagcaggag agcgccgcct accgcgcgct ggcaccgtcg
     1081 cagctggtgc cgtcgctcgt gatcgacgac cagctgctga cgcagtccat ggccatcatc
     1141 gagtacctcg acgagtgtca cccgcagccc agcctgctgc ccggcgacgc gctcgcgcgc
     1201 gcccgcatcc gctcgctcgc gcaggacgtg gcctgcgaga tccacccgct cgacaacctg
     1261 cgcgtgctgc gctacctggt ccacgagatg aaggtcggcg aggacgacaa gacgcgctgg
     1321 taccggcact gggtcgagag cgggctcgag gcgctggagc agcgcctggc cggtgacgca
     1381 cgcaccggcc gcttctgcca cggcgacacg ccgacgctgg ccgactgcgt gctggtgccg
     1441 cagatcgtca acgcgcagcg cttcgactgc cggctcgacc atctgcccac cgtgatgcgc
     1501 gtcttcgacg aatgcatgcg gctggccgcg ttcgccgcca cgcaaccgtc ggcctacccc
     1561 ggaccggcct gatgcgcgac gcgctgctgc gccatccgga ctggctcgcg ccggcgtggg
     1621 acgtggccag ggtcggcgcg ctgatgacga cgcgccacgg cggcgtcagc atggcgccgt
     1681 tcgacagcct gaacctgcgt gccgcggtcg gcgacgaccc gccggcggtc gccgagaacc
     1741 agcgtcggct cgcacaggcg atcggtgcgc ggccggtgtt cctgaaccag gtccatgggg
     1801 ccgcggtcgc gcatctgagc gctgccgatg tgcgggcagg cgctgcactg cacacggccg
     1861 atgcgagcgt cacgaccgag ccgggcgtcg gttgcgcggt gcaggtcgcc gattgcctgc
     1921 cggtgctgtt cgcggcgccg caagggcgcg cggtcggggc cgcgcacgcg ggctggcgcg
     1981 gcctagcgct gggcgtgctg gaggccacgc tcgcggcggt ctgcgcggcg gcgcagtgcg
     2041 agccgggcga ggtgcaggcc tggctcggcg ccggcatcgg gccggcgcat ttcgaggtcg
     2101 gtccggacgt gctcgaggct ttcggtgcct cggctgccgg ctcgccgcgc ttcgttgccc
     2161 gtcagccggg caagtggctc gccgacctgc cggcactggc gcgcgaccgg ctgcaggccg
     2221 ccggcgtgcg cgccgtcagc ggcggcgact ggtgcacggt cgccgacgcg tcacgattct
     2281 tctcgttccg gcgcgaccgc gtcaccgggc gcatggccgc cgtcgtgtgg atcgaggcca
     2341 cccagggccg ctgagcgcgc gtcggcggcg cgccgtgcgc ggcgccgggc cgggctgaag
     2401 aacaggtagg cgacgatgcc cagcggcagc acgccgtaca gcagcagcgt gaacaacgcg
     2461 cccagcagcg tcccgatcgg gctcgccgcc tcgaccacgg cgaccatcag cacgacgtag
     2521 atccaggcga tcgcgatcag cagcatcgaa tgggcctcga tctcatgtat cttattgtga
     2581 aattgaaagt cataacgcga tacgaacgag aatagacttc ggatgtacaa agcgctcgga
     2641 ctcacgtaaa acaagttacc gcgcccgatc aatacaagaa ccgctgcagg agacacatga
     2701 agagcaagcc cgattctgct gccgcgtcgg ttcccgacct ggccgccaat gcacaggcgc
     2761 tgggcgccac gttcagcgag atctggaagt cgatgtcggg cctgagcctg ccgatgcccg
     2821 cggtggccga gctgcagagc gactacctga agcagtcgac cgagctgtgg aaccagaccc
     2881 tgcacgcgca agccgccgcg ccggccgatc gccgtttcgc cgcatccgac tgggcgaaga
     2941 acccggccag cgcctacacc gcgcagatgt acctgctcaa cgcccgcacg ctgatgcaga
     3001 tggccgactg cctgcagggc gacgagaaga cgcggggccg catccgcttc gcgctgcagc
     3061 agtggatcga tgcggcggcg ccgagcaact acctcgcgct gaaccccgag gcacaacgca
     3121 aggcgctcga cacccagggc gagagcatcg cgctgggcat gaagcacctg ctggacgacg
     3181 tgcgccaggg ccacgtctca cagaccgacg aaagcgtgtt cgagatcggc cgcaacgtcg
     3241 cgaccaccga gggcgcgatc gtgttcgaga acgagctgtt ccagctgatc gagtacaagc
     3301 cgctgaccgc gacggtgttc gagcggccga tgctgttcgt gccgccgtgc atcaacaagt
     3361 actacatcct cgatctgcag cccgagaact cggtgatccg ctacaccgtc gcgcagggcc
     3421 accgcctgtt cgtgatcagc tggcgcaatg ccgacgagag catcaagacc tatacgtggg
     3481 atcagtacat cgaagacgcg gcgatccgcg cgatcggcct cgtccaggag atcaccggca
     3541 gcaagcagat caacacgctg ggcttttgcg tcggcggcac gatcctggcg accgcgctcg
     3601 cggtgctggc cgcgcgcgac cggaagccgg ccgccagcat gaccttgctg accaccttcc
     3661 tcgacttcag cagcaccggc gtgctcgacc tgttcgtcga cgagaccatg gtgcggatgc
     3721 gcgagatgac gctcgggccc aagagcccgg gcggaggcag cctgctcaag ggccaggaac
     3781 tcgcgaccac cttcagcttc ctgcgcccga acgacctggt ctggaactat gtcgtcggca
     3841 actacctgaa gggcgagacg ccgccgccgt tcgacctgct gtactggaac agcgacggca
     3901 cgaatctgcc cgggccgat
//
LOCUS       DATMJB010000208         5112 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_865124, whole genome shotgun sequence.
ACCESSION   DATMJB010000208 DATMJB010000000
VERSION     DATMJB010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865124"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VNU48_04605"
     CDS             <1..508
                     /locus_tag="VNU48_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWI10588.1"
                     /translation="AESFLARYDPREVACLIADIRMDGMTGLELQDRLIERKSPLPIV
                     FVTGHGDVPMAVSTMKKGAMDFIEKPFKEDDLVSLVERMLDQARSAFSQHQEQASRDA
                     LLSRLTTREAQVLERIVAGRLNKQIADDLGISIKTVEAHRANIMEKLNANTVADLLKI
                     ALGAQAKV"
     gene            575..1429
                     /locus_tag="VNU48_04610"
     CDS             575..1429
                     /locus_tag="VNU48_04610"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HWI10589.1"
                     /translation="MTAQLIDGNALAKTIRAEVAERAAALSARGHPPGLAVILVGDDP
                     ASQVYVKHKVNDCVAAGIRSVLETYPATFGEAALLARIAALNADPAIHGILVQMPLPA
                     HINPHRVIEAISVAKDVDGFSTLSAGELMTGQPGFRPCTPYGCMKLIATTGVALRGKH
                     AVVIGRSNTVGKPMALLLLQAHATVSICHSGTPDIGKWTRHADVVVAAVGKRELLTAD
                     MVKPGAVVIDVGMNRKADGKLCGDVDFAGVSQVAGWITPVPGGVGPMTRAMLLVNTLE
                     SAERVAAA"
     gene            1458..3548
                     /locus_tag="VNU48_04615"
     CDS             1458..3548
                     /locus_tag="VNU48_04615"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HWI10590.1"
                     /translation="MDTTNPLLDNEGLPRFDAIRPEHIGPALDELLANADAALERATS
                     DAVPATYDALSAVLDVATERLGRAWGAVGHLHAVADSPALRAAYTENMPRVVDFHTRM
                     GADARLFAKYRAVLADPTSAQLPAPRRRALTNALRDFRLSGAELVGAAKARHAALQDL
                     QAETGQKFSEHVLDATDAYAYYASVDEMAGVPADVVQAARNAAAAEGRDGHKLTLHAP
                     RYFPLMQYAHDRALRERLYRAYATRASEFGPAALDNGPLIRQLLAWRQEEAALLGHAS
                     PSEQSLVPKMASSPAQVISFLEDLGRRARPGALRDLAELREFARTELGLPELQAWDLP
                     YASQKLKEKRYAFSEQEVRQYFTEPKVLEGLFRIVEAVFEVRIRPDTAPVWHPSVRFF
                     RIEKAPAPGEEPGAGAAVPRLVGQFYLDPFARPGKRPGAWMDDVRERWLRPEGRLQTP
                     VAHLVCNFAPPVVTDGVERPALLTHDDVTTLFHEFGHGLQLMLTQIDDLGVSGISGVE
                     WDAVELPSQFMENFCWEWDVVKRMTAHVDTGAALPRDLFDRMLAAKNFHSGLDTLRQV
                     EFALIDMRLHTEPGAEQRVAEIVDAVRAAVAVLTPPEFARPLHTFTHIFDGAYAAGYY
                     SYKWAEVLSADAWSAFEESADGEAGVLNVETGRRYREAVLETGGSRSTLDSFKAFRGR
                     EPSIDALLRHQGLG"
     gene            3666..4304
                     /locus_tag="VNU48_04620"
     CDS             3666..4304
                     /locus_tag="VNU48_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWI10591.1"
                     /translation="MKHDCAMTIRLPRWGWAALVVLLAALPAHAQYKVISADGKVTYT
                     DRPPAAGKDKVTTISSRGVGGGAVDVTLPLELRQPVARYPVTLYTVTGTCGPCETARQ
                     LLRQRGVPYAEKQVQTPEDSEALERLSGARDAPTLAIGTQMMRGFAPEVWGSYLDAAG
                     YPRESRLPANYQYAAATPIVERREASAARPAASAAAPDATVAPPASPTGIKF"
     gene            complement(4344..>5112)
                     /locus_tag="VNU48_04625"
     CDS             complement(4344..>5112)
                     /locus_tag="VNU48_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWI10592.1"
                     /translation="SNVKTRGIFGEVQLAGLLEQVFTPDQYALNVETIPGSGARVEFA
                     IKLPSRREDGAPLWLPIDAKFPREDYERLLDAQERADKPEAEAAGRAIEQRLRMEAKT
                     IREKYVAPPHTTEFAILFVPTEGLYAEALRRPGLMEALHREHRVTLAGPTTLLAILGS
                     YQMGFRTLALEKRSAEVWEVLGAVKTEFAKFGEVLAKTKKKLDEASNTIDQAQTRTNQ
                     MARKLKSVEALSDTHAQLLLPIAAPGVEAELTDDESA"
BASE COUNT          772 a         1858 c         1761 g          721 t
ORIGIN      
        1 cgccgaatcc ttcctggccc gctacgaccc gcgcgaagtc gcctgcctga ttgccgacat
       61 ccgcatggac ggcatgaccg gcctggagct gcaggaccgg ctcatcgagc gcaagagccc
      121 gctgcccatc gtcttcgtga ccggccacgg cgacgtgccg atggccgtct cgacgatgaa
      181 gaagggcgcg atggacttca tcgagaagcc gttcaaggag gacgacctcg tctccctcgt
      241 cgagcgcatg ctcgaccagg cgcgctccgc cttctcgcag caccaggagc aggccagccg
      301 cgacgccctg ctctcgcgcc tcaccacgcg cgaggcgcag gtgctcgagc gcatcgtcgc
      361 cggtcgcctc aacaagcaga tcgccgacga tctcggcatc agcatcaaga cggtcgaggc
      421 gcaccgtgcc aacatcatgg aaaagctcaa cgccaacacc gtggccgacc tgctgaagat
      481 cgcgctcggc gcgcaagcca aggtctgaga gactgagccc atcgcgccat cggaaggccg
      541 ccggatcggc ggcctttttg tttgaggaac gtcaatgact gcacaactga tcgacggcaa
      601 tgccctggcc aagaccatcc gcgccgaagt cgccgaacgc gccgccgccc tcagcgcacg
      661 cggccatccg cccggcctcg cggtgatcct ggtcggtgac gatccggcct cgcaggtcta
      721 cgtcaagcac aaggtcaacg actgtgtggc ggcaggcatc cgctcggtgc tcgagaccta
      781 ccccgcgacc ttcggcgagg ccgcgctgct ggcacgcatc gcggcgctga acgccgatcc
      841 ggcgatccac ggcatcctgg tgcagatgcc gctgcccgcg cacatcaacc cgcaccgcgt
      901 gatcgaggcg atctcggtcg cgaaggacgt cgacggcttc tccacgctca gcgcgggcga
      961 gctgatgacc ggccaacccg gctttcgccc ctgcacgccc tacggctgca tgaagctgat
     1021 cgcgaccacc ggcgtcgcgc tgcgcggcaa gcacgcggtc gtgatcgggc gcagcaacac
     1081 ggtcggcaag ccgatggcgc tgctgctcct gcaggcccac gcgaccgttt ccatctgcca
     1141 cagcggcacc ccggacatcg gcaagtggac ccgacacgcc gatgtggtgg tcgccgcggt
     1201 cggcaagcgc gagctgctga ccgcggatat ggtcaagccc ggcgcggtcg tgatcgacgt
     1261 cggcatgaac cgcaaggccg acggcaagct ctgcggcgat gtcgacttcg ccggcgtgtc
     1321 gcaggtggcc ggctggatca cgccggtgcc gggcggcgtc gggccgatga cgcgcgcgat
     1381 gctgctggtg aacacgctgg aatcggccga gcgcgtcgcg gcggcctgat ccggccacac
     1441 acaggatggg cgagcgaatg gacaccacga accccctgct cgacaacgaa gggctgccgc
     1501 gcttcgatgc gatccgcccc gagcacatcg gcccggcgct ggacgaactg ctcgcgaacg
     1561 ccgatgccgc gctcgagcgc gcgaccagcg acgcggtgcc cgcgacctac gacgcgctct
     1621 cggcggtgct cgacgtcgcg accgaacgcc tgggccgcgc ctggggcgcg gtcgggcacc
     1681 tgcatgccgt cgccgattcg cccgcgctgc gcgccgcgta caccgagaac atgccgcgcg
     1741 tcgtcgactt ccacacccgc atgggcgccg acgcgcggct gttcgcgaag taccgggctg
     1801 tgctcgccga cccgacatcg gcgcagctgc cggcaccgcg ccggcgcgcg ctgacgaacg
     1861 cgttgcgcga cttccgcctc tccggcgccg aactggtggg cgcggcgaag gcgcgccatg
     1921 cggcgctgca ggacctgcag gccgagaccg gtcagaagtt ctccgagcac gtgctcgacg
     1981 cgaccgacgc ctacgcctat tacgccagcg tcgacgagat ggccggcgtg cccgccgacg
     2041 tcgtgcaggc cgcgcgcaac gccgccgcgg ccgagggccg cgacgggcac aagctgacgc
     2101 tgcacgcacc gcgctacttc ccgctgatgc agtacgcgca cgaccgcgcg ctgcgcgagc
     2161 gcctgtaccg tgcctatgcc acgcgcgcca gcgaattcgg cccggcggcg ctcgacaacg
     2221 gcccgttgat ccggcagttg ctcgcctggc gccaggaaga agcggcgctg ctcggccatg
     2281 ccagcccgtc cgagcaatcg ctggtgccga agatggcgag ctcgccggcg caggtgatct
     2341 cgttcctcga agacctgggc cggcgcgccc gccccggcgc gctgcgcgac ctggccgagc
     2401 tgcgcgagtt cgcgcgcacc gagctcggcc tgcccgagct gcaggcctgg gacctgccgt
     2461 acgcgagcca gaagctgaag gagaagcgct acgccttcag cgaacaggag gtcaggcagt
     2521 acttcaccga accgaaggtg ctcgaagggc tgttccgcat cgtcgaggcg gtgttcgagg
     2581 tgcgcatccg gcccgacacc gcaccggtgt ggcacccgag cgtgcgcttc ttccgcatcg
     2641 agaaggcgcc cgcgccgggc gaggagccgg gcgcgggcgc cgccgtgccg cgcctggtcg
     2701 gccagttcta cctcgacccg ttcgcgcggc ccggcaagcg ccccggcgcc tggatggacg
     2761 acgtgcgcga gcgctggctg cgacccgagg gccggctgca gacgccggtg gcgcacctgg
     2821 tgtgcaactt cgcgccgccg gtcgtcaccg acggcgtgga gcgtccggcg ctgctgacgc
     2881 acgacgatgt cacgaccctg ttccacgagt tcggccacgg cctgcaactg atgctgacgc
     2941 agatcgacga cctcggcgtc tccggcatct ccggcgtcga atgggacgcg gtcgagctgc
     3001 cgagccagtt catggagaac ttctgctggg aatgggacgt ggtcaagcgc atgaccgcgc
     3061 acgtcgacac cggcgccgcg ctgccgcgcg acctgttcga ccggatgctg gcggcgaaga
     3121 atttccacag cgggctggac acgctgcgcc aggtcgagtt cgcgctgatc gacatgcggc
     3181 tgcacaccga acccggtgcc gagcagcgcg tggccgagat cgtcgacgcg gtgcgcgccg
     3241 cggtcgcagt gctgacaccg cccgaattcg cacggccgct gcacaccttc acgcacatct
     3301 tcgacggcgc ctacgcggcc ggctactaca gctacaagtg ggccgaggtc ttgtcggccg
     3361 acgcctggag cgcgttcgag gaaagcgcgg acggcgaggc cggcgtgctc aacgtcgaga
     3421 ccggccggcg ctaccgcgaa gcggtgctcg agaccggcgg cagccgctcg acgctcgaca
     3481 gcttcaaggc cttccgcggc cgcgagccga gcatcgatgc gctgcttcgg caccaggggc
     3541 tgggttgagc cccgtcgtgg ctcagccgag ccgtgtcttc cggcccacgc actcgggggg
     3601 tgcgcgcttg aaggcccctg ccgcgtgcgg tagattcagg ggtgactgcc gcacccggag
     3661 cccccatgaa gcatgactgc gcaatgacga tccgcctgcc ccggtggggc tgggctgcgc
     3721 tggtggtgct gctcgcggcg ctgccggccc acgcccagta caaggtgatc agcgccgacg
     3781 gcaaggtcac ctacaccgac cggccgccgg cggcgggcaa ggacaaggtc accacgatca
     3841 gcagccgcgg ggtcggcggc ggagcggtcg acgtgacgct gccgctcgag ttgcgccagc
     3901 cggtcgcgcg ctacccggtg acgctgtaca ccgtcaccgg cacctgcggg ccatgcgaga
     3961 cggcgcgcca gctgctgcgc cagcgcggcg ttccctatgc ggaaaagcag gtgcagacgc
     4021 cggaggacag cgaggcgctc gagcgcctgt cgggcgcgcg cgatgcgccc acgctggcga
     4081 tcggcacgca gatgatgcgt ggcttcgcgc ccgaggtctg gggctcgtac ctcgatgccg
     4141 ccggctaccc gcgcgaatcg cgcctgccgg cgaactacca gtacgcggcc gccacgccga
     4201 tcgtcgagcg tcgcgaggcc agcgcggcgc gcccggcggc atcggccgcg gcgccggacg
     4261 cgaccgtggc gccacccgcg agccccacgg gcatcaagtt ctgagcggat cgcgcggcag
     4321 cgcggccggc gcagggcggc ggctcaggcg ctctcgtcat cggtgagttc ggcctcgacc
     4381 ccgggcgcgg cgatcggcag cagcagctgc gcgtgcgtgt ccgacagcgc ctcgaccgac
     4441 ttcagcttgc gcgccatctg gttcgtgcgc gtctgcgcct ggtcgatcgt gttgctggct
     4501 tcgtcgagct tcttcttggt cttcgcgagc acctcgccga acttcgcgaa ctcggtcttg
     4561 accgcgccca gcacctccca gacctcggcg ctgcgcttct ccagcgccag cgtgcgaaag
     4621 cccatctgat agctgccgag gatcgccagc agcgtggtcg gcccggcgag cgtgacgcgg
     4681 tgttcgcgat gcagcgcctc catcaggccg ggccggcgca gcgcctccgc gtacagccct
     4741 tcggtcggca cgaacaggat cgcgaattcg gtcgtgtgcg gcggcgccac gtacttctcg
     4801 cggatcgtct tcgcttccat gcgcaggcgc tgttcgatcg cgcggcccgc ggcctcggcc
     4861 tcgggcttgt cggcgcgctc ctgcgcgtcg agcaggcgct cgtagtcctc gcgcgggaac
     4921 ttcgcgtcga tcggcagcca cagcggcgcg ccatcctcac gccggctcgg cagcttgatc
     4981 gcgaactcga cgcgcgcacc gctgcccgga atggtctcga cgttgagggc gtactggtcg
     5041 ggcgtgaaca cctgctccag cagcccggcc agctgcacct cgccgaagat gccgcgcgtc
     5101 ttgacgttcg ac
//
LOCUS       DATMJB010000209         3360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_369275, whole genome shotgun sequence.
ACCESSION   DATMJB010000209 DATMJB010000000
VERSION     DATMJB010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369275"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3360)
                     /locus_tag="VNU48_04630"
     CDS             complement(<1..>3360)
                     /locus_tag="VNU48_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWI10593.1"
                     /translation="TTTTLADIGGSIVIGPLTLADVQAESLAGSRGVTAYALDASRTE
                     AVGVSASGQSSADHSWNYFDGNGWRTFSNESGGVWTRYFYDGAGNVSKEVRFQRRDAQ
                     GSFEGAITDQAERPTLAQLEADYAAAIAAHAAGADTVRVTTRSFDAAHNLLGETEHST
                     AFGAVSTRRAYDRYNNQVLDVSAVGIAGLENRLATEYDAFNRVVRTETGAFAHLDEAG
                     NAAPAGGAVNSFGYDARGNQSTHLDARGFTERMHYDAYNRLASQWDGQRADSASTLRT
                     DRVYDAFDRLIELRANDLTGRAGQAVQVSTFQWSAFDQQLAHTDALGHRVQRSYDLAG
                     NQTSETDALGNTERYAFDADGHMVQRTDRLGGISSTSYNAYGQRNSETDANGRVTTWT
                     LGAFGQITGSSTGFTNGYARLAGAAASTETMLHDWLGRLTQTSDSFGKQIEYTYDDAD
                     HQTRIEDLTNDRAVAYAYDANGQRTSETLTEAGLTTRHQTNHYNSQGWLSGVDADAGF
                     DAGGGAVLGQQLSVAYTFDAAGNRVRIDDGHDDGHYSFDASGRMTRGVDAKRNEVVSQ
                     LVYDGYGNRLSETQAGVQTTYTYDAANRVAASSVGEAWAYDANGNTTYTRARNGDTVS
                     TQYNAENRATTTVSSSAGKTTTSTNGYDAVGNVLQTHVQGDGYGFDEITLRDVRYLEQ
                     SKTIANSWASGAERLEGSTSFRYDSNGNLTLLDRGRKQGAGENSVAVFEYDLEGHIIG
                     RADKATALTAGDFFAGYPSDPDLAPVYDEYGAGQSVTQQVQSQLFGAGTGATTHLQNY
                     LYANNKSIAQAEGDQVISLHTLRLVGGQAVTDAEQHVVGWQLTLQASDVASTNGQIDR
                     TQTARNIAARAYDGFSALSATAQAKIVAYVQAQLPADAQLTAGTVIDLHGFIQLTDAS
                     IANVTQLSDYSLRQLGTDGLPGGNVQSHVVRAGDTLQSIAAMYFGSPSYWYLIADANG
                     LSGSEPLVEGTTLTIPNAVANSVNTADTFKVYNESEIIGSTSPEIRTIQKKKKWWQTL
                     VMIIIVVILIIATIYTAGAASGAWGAAGASAGAAGGAAAAGTAVAGTAVAGTAAATTI
                     GGSIFAAGAALGSSLAAGLGYAGALAVAAGVG"
BASE COUNT          350 a         1038 c         1317 g          655 t
ORIGIN      
        1 gccgacgcct gcggccaccg ccagcgcgcc ggcgtagccc agccccgcgg ccaggctgct
       61 gcccagcgcg gcgccggccg cgaagatgct gccgcctatg gtggtggcgg cggccgtgcc
      121 cgcaaccgcg gtgccggcta ccgccgtgcc cgcggcggct gcccctccgg ccgcgccggc
      181 gctcgctcct gcggctcccc acgctcccga cgccgcgccc gcggtgtaga tcgtggcgat
      241 gatcaggatc accacgatga tgatcatcac cagcgtctgc caccacttct tctttttctg
      301 gatggttcgg atctcggggc tggtgctgcc gatgatctcg ctctcgttgt agaccttgaa
      361 ggtgtcggcg gtgttgacgc tgttcgctac tgcgttgggg atggtcagcg tcgtgccctc
      421 gaccagcggc tcgctgccgc tcaatccgtt ggcgtcggcg atcaggtacc agtagctggg
      481 gctgccgaag tacatcgccg cgatgctttg cagcgtgtcg ccggctctga cgacgtggct
      541 ttgcacgttg cccccgggca gcccgtcggt gccgagctgg cgcaggctgt agtcgctgag
      601 ctgggtgacg ttggcgatgc tcgcgtcggt gagctggatg aagccgtgca gatcgatcac
      661 ggtgccggcg gtcaattgcg cgtcggccgg cagctgggcc tgcacgtagg cgacgatctt
      721 ggcctgtgcg gtcgcgctca gggcgctgaa gccatcgtag gcgcgcgcgg cgatgttgcg
      781 ggcggtctgg gtgcggtcga tttgaccatt ggtgctggcg acgtcgctcg cctgcagcgt
      841 gagctgccag ccgaccacgt gctgctcggc gtccgtcacg gcctggccgc ccaccaggcg
      901 cagcgtgtgc aggctgatca cctggtcgcc ctcggcctgg gcgatgctct tgttgttggc
      961 gtacaggtag ttctgcagat gggtggtggc gccggtccct gcgccgaaga gctggctctg
     1021 cacctgctgg gtgacgcttt ggccggcgcc gtactcgtcg tagacggggg cgaggtcggg
     1081 gtcgctcggg tagccggcga agaagtcgcc cgcggtcagc gcggtggcct tgtcggcgcg
     1141 gccgatgatg tggccttcga ggtcgtactc gaacacggcc acggagttct cgccggcgcc
     1201 ttgcttgcgc ccgcggtcga gcagggtcag gttgccgttg ctgtcgtagc ggaagctggt
     1261 ggagccctcc agccgctcgg cgccgctggc ccagctgttg gcgattgtct tgctctgctc
     1321 gaggtagcgc acgtcgcgca gggtgatttc gtcgaagccg tagccgtcgc cttgcacgtg
     1381 ggtctgcagc acgttgccga ccgcgtcgta gccgttggtg ctggtggtgg tcttgccggc
     1441 agagctgctg acggtggtgg tggcgcggtt ctcggcgttg tactgggtgc tgacggtgtc
     1501 gccgttgcgg gcgcgggtgt aggtggtgtt gccgttggcg tcgtaggccc aggcttcgcc
     1561 gacgctgctg gcggcaacgc ggttggcggc gtcgtaggtg taggtggtct gcacgccggc
     1621 ttgggtttcg gagaggcggt tgccgtagcc gtcgtagacg agctggctga cgacctcatt
     1681 gcgcttggca tcaacgccgc gggtcatgcg gccggaggcg tcgaagctgt agtgcccgtc
     1741 gtcgtgcccg tcgtcgatgc gcacccggtt gccggcggcg tcgaacgtgt aggccacgct
     1801 gagctgctgg ccgagcacgg ccccgccgcc ggcgtcgaag ccggcgtcgg cgtccacgcc
     1861 gctgagccag ccctggctgt tgtagtggtt ggtctggtgg cgcgtcgtga ggccggcttc
     1921 ggtgagggtt tcggaggtgc gctggccgtt ggcgtcgtag gcgtaggcga cggcgcggtc
     1981 gttggtcagg tcctcgatgc gggtctggtg gtcggcgtcg tcgtaggtgt attcgatctg
     2041 cttgccgaag ctgtcgctgg tttgcgtgag gcgaccgagc cagtcgtgca gcatggtctc
     2101 ggtgctggcg gcggcgccgg ccaggcgggc gtagccgttg gtgaagccgg tgctgctgcc
     2161 ggtgatctgg ccgaaggcgc ccagggtcca ggtggtgacg cggccgttgg cgtcggtttc
     2221 ggaattgcgc tggccgtagg cgttgtagct ggtgctgctg atgccgccga gccggtcggt
     2281 gcgctgcacc atgtggccgt cggcgtcaaa ggcgtagcgc tcggtgttgc ccagcgcgtc
     2341 ggtctcgctg gtctggttgc cggcgaggtc gtagctgcgc tgcacgcggt ggccgagggc
     2401 gtcggtgtgc gcgagctgct ggtcgaacgc gctccactgg aaggtgctca cttgcacggc
     2461 ctggccggcc cggccggtca ggtcgttggc gcgcagctcg atcaggcggt cgaaggcgtc
     2521 gtagacgcgg tcggtgcgca gggtcgaggc gctgtcggcg cgctgcccgt cccattggct
     2581 ggccaggcgg ttgtaggcgt cgtagtgcat gcgctcggtg aagccgcgcg catccaggtg
     2641 ggtgctctgg ttgccgcggg cgtcgtagcc gaagctgttg acggcgccgc cggcgggggc
     2701 ggcgttgccg gcttcgtcga ggtgcgcgaa cgcgccggtc tcggtgcgca cgacgcggtt
     2761 gaaggcgtcg tattcggtgg cgagccggtt ctccaggccg gcgatgccga ccgcgctgac
     2821 gtcgagcacc tggttgttgt agcggtcgta ggcgcgccgc gtgctcaccg cgccgaaggc
     2881 ggtggagtgc tcggtctcgc cgagcaggtt gtgggcggcg tcgaagctgc gggtggtcac
     2941 gcgcacggtg tcggcgccgg cggcgtgcgc ggcgatggcc gcggcgtagt cggcttcgag
     3001 ctgggccagc gtggggcgct cggcctggtc ggtgatggcg ccctcgaagc tgccctgcgc
     3061 gtcacggcgc tggaagcgaa cctccttgct gacgttgccg gcgccgtcgt agaagtagcg
     3121 cgtccacacg ccgccgctct cgttcgagaa ggtgcgccag ccgttgccgt cgaagtagtt
     3181 ccagctgtgg tcggcgctgc tctggccgct cgcgctgacg ccgacggctt cggtgcggct
     3241 cgcgtcgagc gcgtaggcgg tgacgccgcg gctgcccgcg aggctctcgg cctgcacgtc
     3301 ggccagcgtc agcgggccga tcacgatgct gccgccgatg tcggccagcg tcgtggtggt
//
LOCUS       DATMJB010000210         4043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_229867, whole genome shotgun sequence.
ACCESSION   DATMJB010000210 DATMJB010000000
VERSION     DATMJB010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229867"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /gene="rmuC"
                     /locus_tag="VNU48_04635"
     CDS             complement(<1..533)
                     /gene="rmuC"
                     /locus_tag="VNU48_04635"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWI10594.1"
                     /translation="MNEILFMIGEWPIRGGDALIGFGTLALLLLLTIAIVIARSGRRG
                     AELAMAQAIRADELEERLGEMLRAQSEATGRVDAMGQALAGRQADMARAVNERLDSVT
                     HRVGQSMEATTRNTMDSLRVLHERLGIIDNAHKNLTDLTSQVTTLRDVLANKQSRGAF
                     GQARMEAIVQDGMPQGS"
     gene            724..1251
                     /gene="def"
                     /locus_tag="VNU48_04640"
     CDS             724..1251
                     /gene="def"
                     /locus_tag="VNU48_04640"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003804827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWI10595.1"
                     /translation="MAIREIIILPDKQLRLVSRPVEKVTAEIRKLADDMFETMYDAPG
                     IGLAAIQVAQPLRLITMDLAKRDEDGETTPRPRVFINPEIVWSSEELSVYEEGCLSIP
                     EYYEEVERPAQVRVRFTDLDGKVHEEDADGLFATCIQHEIDHLNGVLFVDYLSKLKRD
                     RVMKKFTKAAKRAAE"
     gene            1458..2369
                     /gene="fmt"
                     /locus_tag="VNU48_04645"
     CDS             1458..2369
                     /gene="fmt"
                     /locus_tag="VNU48_04645"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWI10596.1"
                     /translation="MGTPEFAVPTLLELVAHGHEIAAVYTRAAKPAGRGMKLQPTPVE
                     REARRLGIPVLTPTSLKTPDAQDEFRAHNADAAVVVAYGMILPQPILDAPPLGCFNLH
                     GSLLPRWRGAAPINRAIMSGDAETGVMVMKMDLGLDTGDVAMAERLAITDAMTATDLH
                     DALAPLGADLMVRAMGALERGKLQFAEQSETGVTYAAKIEKAEARIDWHRPARAVLRH
                     IHGLSPFPGAWCEFVGDGEALRVKILRCEPAEGSGAPGDLLDDRLAIACGEGAIRFLE
                     LQRAGKAPMKATEFLRGTPLKPPMRLL"
     gene            2369..3106
                     /gene="truA"
                     /locus_tag="VNU48_04650"
     CDS             2369..3106
                     /gene="truA"
                     /locus_tag="VNU48_04650"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWI10597.1"
                     /translation="MPRYKLLIEYDGAPFSGWQVQDNAPSVQGALETAVKAICGEHVR
                     VHGAGRTDAGVHALGQVAHCDISKHFVPGRFRDGLNAHLRPHPIGVISADIASDDFEA
                     RFSAKKRHYLYRISNRRANLALDIGHAWRLPRPLDTDAMHAAAQRLVGKHDFTTFRDT
                     ECQAKSPEKTLDQLDVVRQGDAVNIVTSARSFLHSQVRSMVGSLVWVGEGRWSADDLA
                     AALAAKNRTACGPVAPPDGLYLVRVDY"
     gene            complement(3149..>4043)
                     /gene="dapE"
                     /locus_tag="VNU48_04655"
     CDS             complement(3149..>4043)
                     /gene="dapE"
                     /locus_tag="VNU48_04655"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF009557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HWI10598.1"
                     /translation="FSGDIKDGFLYGRGAVDMKGGIACSVAAVLDYLADNGGQPKGSI
                     SFLITGDEEAISVNGTVKLLKWAEARGEKFDHCVLGEPSNVEELGDCIKIGRRGSLSG
                     TLYVDGIQGHVAYPHRASNPVPDISRLIVALSDEPLDHGSAQFQASNLEFTSVDVGNT
                     ASNVIAGQARARFNIRFNDCHTQETLRALVEARMTKATGNRIRARIEWEYSNANVFVT
                     KPGAFTDLAVSAIEEITGRKPELSTSGGTSDARFIASYCPVIEFGLVGQTMHQIDERT
                     PVADLEKLTKIYRGVLDRYFA"
BASE COUNT          733 a         1273 c         1303 g          734 t
ORIGIN      
        1 taagaaccct gcggcatgcc gtcctggacg atcgcttcca tccgcgcctg gccgaacgcg
       61 ccgcgggatt gcttgttggc aagcacgtcg cgcagcgtgg tcacctgcga ggtgagatcg
      121 gtgagatttt tatgcgcatt gtcgatgatg ccgagtcgct cgtgcagtac gcgcaggctg
      181 tccatggtgt tgcgcgtggt cgcttccatc gactggccga cccgatgggt caccgaatcc
      241 agccgctcgt tgaccgcgcg tgccatgtcg gcctgacggc cggccagcgc ctgtcccatc
      301 gcatcgacgc gcccggtagc ctcagactgg gcccggagca tctcgcccag ccgctcttcc
      361 agttcgtcgg ctcggatcgc ctgtgccatt gccagttcgg cgccgcggcg gccggagcgc
      421 gcgatcacga tggcgatagt caggagcagc agcagcgcca gcgtgccgaa gccgatcagc
      481 gcgtcaccgc cgcggatcgg ccattcgccg atcatgaaaa ggatctcgtt catgccgccc
      541 ttgtagccga ttcgcctgat aacacgaacg aagggggaac atttatggtg aataacgggt
      601 taatttttat ggttaacaaa gtcctgacat tcatggttac cggcgggtaa acagccgccg
      661 tcagcgcatt gaccccggca aaccggcagc ttaaatcgcg agcccgggac acctagaaaa
      721 aacatggcca ttcgcgaaat catcattctg cccgacaaac agttgcggct cgtctccagg
      781 cctgtcgaaa aggtcacggc ggagattcgc aagctggccg acgacatgtt cgagaccatg
      841 tacgacgcgc ccggcatcgg gctggcggcg atccaggtcg cgcagccgct gcggctgatc
      901 acgatggatc tcgccaagcg cgacgaggac ggcgagacta cgccgcggcc gcgcgtcttc
      961 atcaatccgg aaatcgtctg gtcgtcggaa gaattgtcgg tgtacgagga aggctgccta
     1021 tcgatccccg aatattacga ggaggtggag cgaccggcgc aggtgcgtgt gcgcttcacc
     1081 gatcttgacg gcaaggtaca tgaggaagac gccgacgggc tgttcgctac ctgcatccag
     1141 cacgagatcg atcatctcaa cggcgtgctg ttcgtggatt atctgtcgaa gctgaagcgc
     1201 gatcgcgtga tgaagaagtt tacgaaggcg gcgaagcgcg cggcggagta gcttatcgat
     1261 cgcgaggctc gcagccgtca tgcccggcct tgtgccaggc atccacgtcc ttcttgccaa
     1321 tcctcaagag cgtggatggc cgggagcgca gacaagtgta cgtagtctgc gtaaggcaga
     1381 cttccatgcc cggccatgac gagtaaactg aatcttcgct aggacgcttc cacgccatgt
     1441 cactccgctt gatcttcatg ggcacgccag aattcgcggt gccgacgctg ctcgaactcg
     1501 tcgcccatgg ccatgagatc gcggcggtct atacccgcgc ggcaaagccc gccgggcgcg
     1561 gcatgaagct gcagccgacc ccggtcgagc gggaggcgcg gcggcttggc attcccgtgc
     1621 tgacgcccac cagcctgaag acaccggacg cgcaggacga gtttcgcgcg cataacgccg
     1681 atgccgccgt ggtcgtcgcc tatggcatga tcctgccgca gccaatcctc gatgcgccac
     1741 cgctcggctg cttcaatttg cacggctcgt tgctgccgcg ctggcgaggt gccgcgccga
     1801 tcaaccgcgc catcatgtcg ggcgatgccg agaccggcgt gatggtgatg aagatggacc
     1861 tcggcctcga taccggcgat gtcgcgatgg ccgagcggct ggcgatcacc gacgccatga
     1921 ccgcgaccga cctgcatgac gcgctggcgc cactcggggc cgacctgatg gtgcgcgcga
     1981 tgggcgcact ggagcgcggc aaattacagt tcgccgaaca aagcgaaacc ggcgtgacct
     2041 atgccgccaa gatcgagaaa gccgaggcgc ggattgactg gcacagaccg gcgcgcgccg
     2101 tgctgcgcca tatccacgga ttgtcgccgt ttcccggcgc gtggtgtgag tttgtcggcg
     2161 acggcgaagc gctgcgcgtc aagatattgc gttgcgaacc ggccgagggt tcgggcgcgc
     2221 cgggcgatct gctcgacgat cgcctcgcca tcgcctgcgg cgaaggcgcc atccgatttc
     2281 tggagctgca gcgcgccggc aaggcgccga tgaaggcgac cgagtttttg cgcggcacgc
     2341 cgctcaagcc gccgatgcgg ctactctgat gccccgctac aaactcctca tcgaatatga
     2401 cggcgcgccg ttttccggct ggcaggttca ggacaacgcc ccgtcggtac agggcgcgct
     2461 ggagacggcg gtcaaggcga tctgcggcga gcatgttcgt gttcatggcg ccgggcgcac
     2521 cgatgccggc gtgcatgcgc tcgggcaggt ggcgcattgc gacatatcaa agcatttcgt
     2581 gccgggccgg tttcgcgacg gcctcaacgc gcatcttcgg ccgcatccga tcggcgtgat
     2641 cagcgccgat atcgcttcag atgatttcga ggcgcggttc tcagccaaga agcgccacta
     2701 cctttatcgg atcagcaatc gccgcgccaa tctggcgctc gacatcggcc atgcctggcg
     2761 gctgccaagg ccgctcgata ccgatgcgat gcatgccgcc gcccagcgcc tcgtcggcaa
     2821 gcacgatttt acgacattcc gcgacaccga atgccaggcg aaatcgccgg agaagactct
     2881 cgaccaactc gacgtggtca ggcagggcga tgccgtgaac atcgtgacct cggcgcgctc
     2941 gttcctgcac agccaggtgc gctcgatggt gggatcattg gtctgggtcg gcgaaggccg
     3001 ctggagcgcc gatgatctcg cggcggctct ggccgcaaaa aaccgcaccg cctgcggccc
     3061 ggtcgcgccg ccggacggac tctatctggt gcgggtggat tactgagtta gatccagccc
     3121 cccgcgagcg gggagaagga gagagccctc acgcaaaata ccggtccagc acgccgcgat
     3181 agatctttgt cagcttttca agatccgcca ccggggtgcg ctcgtcgatc tggtgcatgg
     3241 tctggccgac caggccgaac tcgatcaccg gacaatagct cgcgataaag cgtgcatccg
     3301 aggtgccgcc gctggtggaa agctccggct tgcggccggt gatctcttcg atcgccgaga
     3361 ccgcgagatc ggtgaacgcg ccgggctttg tgacgaacac gttggcgttg gaatattccc
     3421 attcgatgcg cgcgcgaatc cggttgccgg tcgccttggt catgcgcgcc tcgaccagtg
     3481 cccgcagcgt ttcctgggtg tggcagtcgt tgaagcggat gttgaacctg gcgcgcgctt
     3541 gccccgcgat cacgttggag gcggtgttgc cgacatcgac cgaggtgaac tcgagattcg
     3601 aggcctggaa ttgcgcgctg ccgtgatcga gcggctcgtc gctcagcgcc acgatcaatc
     3661 gcgaaatatc cggcaccgga ttggatgcgc ggtgcggata ggcgacatgg ccctggatgc
     3721 cgtcaacgta aagcgtgccc gacagcgagc cgcggcggcc gatcttgatg cagtcgccga
     3781 gctcttcgac attgctgggc tcgccgagca cgcaatggtc gaatttctcg ccgcgcgcct
     3841 cggcccattt cagcagcttg acggtgccgt tgaccgagat cgcttcctcg tcgccggtga
     3901 tcaggaacga gatcgagcct tttggttggc cgccgttgtc agcgagatag tccagcaccg
     3961 cggcgacgct gcaggcgatg ccgcccttca tgtcgacggc gccccgtccg tagagaaaac
     4021 cgtccttgat gtcgcccgag aac
//
LOCUS       DATMJB010000211         8441 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_369241, whole genome shotgun sequence.
ACCESSION   DATMJB010000211 DATMJB010000000
VERSION     DATMJB010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369241"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="VNU48_04660"
     CDS             complement(<1..1021)
                     /locus_tag="VNU48_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10599.1"
                     /translation="MLNDLKPLVVVLTLALIVFALARPICLHFMEAQAFARRRNVWLG
                     LTCAAFVAPNLWLYAAVALPLVYWAGRVDRHPTAVYLLLLLAVPPVRLPIPMPLINQL
                     FELSNFRILELALLVPAAFRILGARDAGQRRPGKADLLLLAFGVLQLVLYMPYEAVTN
                     TMRRAFLFMLDTYALYFVFSRGAANREALRDTMCSFWLACAIVAVIAVFETLRGWLLF
                     TGIPARWGVPNTFSWLLRGDSLRAQVSVGHSLTMGYVLAIAFGFWLYLGGRLESTRNA
                     WLVGIGLWLGLLAAYSRAPWLVAALAGCIYMLASPAGRRGLVRAVIGLVLIGVPFLMT
                     PQGAHL"
     gene            complement(1097..2224)
                     /locus_tag="VNU48_04665"
     CDS             complement(1097..2224)
                     /locus_tag="VNU48_04665"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HWI10600.1"
                     /translation="MTEPGGFGFVRAVEMKTNLRVSRSRIAASKSARAARACAGYSKW
                     QLYTCGHRTCTKTRPDWATIGRLSRPGSGPSPREHAGVEQLNPVLPIDASNGLHLDAT
                     AARGIGESLSAQYCFAEPFPHIVLDNFLPPGVLQTAIDNFPAQSLRSDVVFEEGYAGF
                     HKRQILPEDCNGPARSLFHFFNSQPFLEFLEGLSAIDGLIPDPHYIGGGYHEIAKGGK
                     LGIHADFRINNRLKLSRRMNVIIYLNEGWNEQWGGALELWDRKMTKKCASVAPLVNRC
                     VIFNTDEDSYHGHPDPLESPEGVFRRSIALYYYTASKAIYDELPNRSTMYQARPGESA
                     ANRREALNLRVDQHLKQWVPPALLRYVMALKRRLVSGKRAA"
     gene            2265..2966
                     /locus_tag="VNU48_04670"
     CDS             2265..2966
                     /locus_tag="VNU48_04670"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HWI10601.1"
                     /translation="MTMKLNTTALTLTVLMCSASVAGFTMRPDTTAASKGNAVSLESL
                     VPKRFGDWVELPEQGAQVVNPQTQELLDKLYSQLLVRTYVNKEGYRIMLSMAYGDDQR
                     GGLQAHRPEVCYPAQGFKLGKVEDGLLPTSYGNIDVRRLATSLGARNEPVTYWLTVGD
                     QVIKNTLDKRIAEIRLGLTGQIPDGLLFRISSIDNDNARGFEMQRKFAAEMMSAVPAA
                     ARKQLSGLAPAPAAS"
     gene            complement(3021..3881)
                     /gene="xrtB"
                     /locus_tag="VNU48_04675"
     CDS             complement(3021..3881)
                     /gene="xrtB"
                     /locus_tag="VNU48_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR03113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase B"
                     /protein_id="HWI10602.1"
                     /translation="MSSVLRDNSSEAGVKLPTAWLVALAGFAAMYLPLYWWAANGIWK
                     SEEHGHGAIILAVMLWLFWTLRHEIDQAPVKPVPALGWPLFGVGLFVYFVGRVFDISI
                     LEFASQPLVVAGALLLMRGSAAIRLAWFPLLYFIFMIPLPGILVDAITGPLKSQIANI
                     VESLLYAVGYPIARSGVILSIGQYQLQVADACSGLHSMFSLAALGTLFMYLMGRRSWP
                     HRAVMLASILPIAFAANIVRVITLVLVTYYLGDEAGQGFLHGAAGMVLMLVALAFFFF
                     LDWLLDKWLP"
     gene            complement(3878..4792)
                     /gene="epsG"
                     /locus_tag="VNU48_04680"
     CDS             complement(3878..4792)
                     /gene="epsG"
                     /locus_tag="VNU48_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein tyrosine kinase
                     EpsG"
                     /protein_id="HWI10603.1"
                     /translation="MKMTVSMLSGAPRVSRSLGGILIDSGLLKPEDAERVLLVQKEHN
                     LRFGDAAVRLGLLTEADIQYALSRQFAYAYLRKTPGDKKPVSDELVAAYEPFSSRVEE
                     LRAVRSQLMLRWFDKADQRQVLTIIGAERGEGRSHLAANLAVVFSQLGERTLLVDADL
                     RHPRQHELFYLENKIGFSTVLSGRSREEAIVRIPDLAGLCVLPAGPVPPNPLELLNRL
                     NFDEFLIQAKATFDIVIIDTPALSVGEDAAMVAVRTGAALAVARNSQTKVSGFTDMVH
                     GLMNAGVSVVGSVLNEVPAGKPKKEKGP"
     gene            complement(4789..6213)
                     /gene="epsF"
                     /locus_tag="VNU48_04685"
     CDS             complement(4789..6213)
                     /gene="epsF"
                     /locus_tag="VNU48_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR03017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chain length determinant protein EpsF"
                     /protein_id="HWI10604.1"
                     /translation="MSIKQILRVVWARKWLVLALLVVISVAGTAITLLLPKQYTAESA
                     MIVEMRIDPVLGAIAPGLAVPGYLATQVDILKSERVASRVVKMLGVERSPAAVAQWRD
                     QTNAKIPLERYFANLLQRGLAVEPGRGSNVINVSFSAQDPIFAQAAANAFVQAYMDIS
                     VELRVAPARQSAAFLDDQTKVLRNNLETAQARLSKFQQDKGIVVSDERFDQENARYQA
                     LTAQLSAAQAERVDAETRNRNSGGQSSPDILSSPAVQMLKSQLATGEARMSEMSSIWG
                     KNHPERIRLEAQLNELRAQIAAESRRVSGGTSTVSRTSGQKVAELQAMVDEQKKKLQT
                     LRADRDLEAVIKRDVDTAQRAYEGVSTRVGQFNLESQNNQANTRLLSPAVEPLEASRP
                     KVAVGIAGSIIGGLVLGVLAALGWELIDRRVRDPEDLMVMAGVPVLGVLRPEGSKRPV
                     FRRLLQVGPAGPGRPLLAAPGVRS"
     gene            complement(6234..7061)
                     /gene="epsE"
                     /locus_tag="VNU48_04690"
     CDS             complement(6234..7061)
                     /gene="epsE"
                     /locus_tag="VNU48_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein EpsE"
                     /protein_id="HWI10605.1"
                     /translation="MPNLFRSVLLALTLCCGVAFAQTSPSATAPAAKAEALLGAGDVV
                     KITVYQNPDLTVDGARISETNQINFPLVGNVVIGGLTASAAEQKIAKLLRDGGFVLRP
                     QVTIQVGQIRSAVISILGQVGKPGRYPIETVGAKVSEMVAAAGGVVPGGADVVTLVGT
                     RNGKPIKLDIDLPAILQSGKAELDLEVQNGDVIYADRAPTAYIYGEVQRPGQFKLERG
                     MTLMQALAQSGGLTARGTQRGIKVHRRDANGAVEIISLDLNDPVARDDVIYVKESLF"
     gene            complement(7120..8136)
                     /locus_tag="VNU48_04695"
     CDS             complement(7120..8136)
                     /locus_tag="VNU48_04695"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsD family peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWI10606.1"
                     /translation="MTLAALTATVLAVALTGCGEKKDKPAASQTAAKVNKEEITVHQI
                     NFVLQQQRGLAPEQAASASKVALERLIDQELALQKAQDQKLDRDPRVVQQLEAARREI
                     IARAYVDKIAAGAPKPSAAEIKAYYEAHPALFKERRVYNLQELAIQARPEQVAELQAK
                     LGASKDVPDFVNYLKANDFKFAANQAVRGAEQLPLASLDRFAQLKDGQTIFSATPTGA
                     QVVIMAGARSQPVDEAHAVPAIEQFLWNERKRKVIEDDVKALRGAAKIEYVGDYAKGG
                     SKEVKPPPPAASEAPPLTSVAPAMPAGSIPSAAPQVEVEPINTAPASSPTTGTLEKGI
                     KGFK"
BASE COUNT         1431 a         2914 c         2755 g         1341 t
ORIGIN      
        1 ccagatgagc accttgcggt gtcatcagga acggcacgcc gatcaagacc agcccgatga
       61 cggcgcgcac caaccctcgc cgcccggcgg gcgaggcgag catgtagatg cagcccgcca
      121 aggcagccac gagccacggc gcgcgcgagt acgcggcaag cagacccagc cacagcccga
      181 tgcccacgag ccaagcattg cgcgtcgatt cgagccggcc acccagatag agccagaagc
      241 caaacgcgat cgccagcaca tagcccatcg tcagagagtg tccgaccgag acctgggccc
      301 gcagcgaatc gcctcggagc aaccacgaga acgtgttcgg cacaccccat cgggcaggaa
      361 tccccgtgaa caacagccag cccctcaagg tttcgaagac ggcgatgaca gcgacgatcg
      421 cgcaagccag ccagaagctg cacatcgtgt cgcgcagcgc ctcccgattc gccgcgccac
      481 ggctgaagac gaagtagagc gcgtaggtgt cgagcatgaa caggaacgcg cgccgcatcg
      541 tgttggtcac ggcttcatag ggcatgtaca gcacgagctg cagcacgccg aaggccagca
      601 gcagcagatc cgccttgccc gggcgacgct gccccgcatc gcgcgcgccc agaattcgaa
      661 acgcggccgg gacgagcagc gccaactcga ggatgcgaaa gttgctgagt tcgaacagct
      721 ggttgatcag cggcatcggg atcggcaagc gcaccggcgg cacggccagc agcagcagca
      781 ggtacacggc cgtggggtga cggtcgacgc gcccggccca gtagacgagc ggcagcgcga
      841 cggcggcata cagccacagg ttgggcgcca cgaaggcggc gcaggtcagg ccgagccaga
      901 cattgcggcg ccgggcgaac gcctgcgctt ccatgaagtg caggcagatg ggccgggcca
      961 gcgcgaacac gatcaacgcg agcgtcagca ccaccaccag gggcttcagg tcgttaagca
     1021 tggcgctttg ctccgacgac gcggcgcgcc accgtcggcg gcgccgcgcc cggcgcgtag
     1081 cgaaccgcgc cggccatcac gcggcgcgct tgcccgagac gagccgccgc ttcagcgcca
     1141 tcacataacg cagcaatgcg ggaggaaccc actgcttcag atgctggtcg acgcgcaggt
     1201 tcaacgcttc gcgccgatta gcggcacttt cgcccggccg cgcctggtac atcgtcgacc
     1261 ggttcggcag ctcgtcgtag atggccttcg acgcggtgta gtagtacagg gcgatcgagc
     1321 ggcgaaacac gccttccggc gactcgagcg gatcggggtg cccgtggtac gagtcctcat
     1381 cggtgttgaa aatgacgcag cgattgacca gcggtgcaac cgacgcgcac ttcttcgtca
     1441 tcttgcggtc ccacaactcg agagccccgc cccattgctc gttccagcct tcgttcaggt
     1501 agatgatcac gttcatccgg cggctcagct tcagccggtt gttgatgcgg aagtcggcgt
     1561 ggatgcccag cttgccgccc ttggcgatct cgtgataacc gccgccgatg taatgcgggt
     1621 cggggatcag gccgtcgatc gcggacaggc cctcgagaaa ctcgaggaag ggctgcgagt
     1681 tgaagaaatg gaacagcgag cgcgccggcc cgttgcagtc ctcggggagg atctggcgct
     1741 tgtggaagcc ggcgtagccc tcctcgaaga ccacgtccga gcgcaggctc tgcgccggga
     1801 agttgtcgat cgcggtctgc agcacgccgg gcggcaggaa gttgtcgagg acgatgtgcg
     1861 ggaacggctc ggcaaagcag tattgtgcgg agagcgactc accgatgccg cgcgccgccg
     1921 tggcatcgag atgcaatccg ttcgaggcgt cgatgggtag gaccgggttc aactgttcaa
     1981 ctcctgcgtg ctctcgaggt gacggcccac tgccggggcg gctcaaacgg ccgattgtgg
     2041 cccagtcggg ccgggttttc gtgcaggtcc gatggccgca cgtgtacaac tgccacttcg
     2101 agtaacccgc gcacgcgcgc gccgcccggg cagacttgga cgcggcaatg cggctgcgcg
     2161 aaacgcgcaa attcgtcttc atttcgaccg cgcgaacgaa accaaacccg cccggctcgg
     2221 tcacaatttg ccgtccttgc gtgcacccgc gcctcaaccc gacgatgacc atgaaactga
     2281 acaccaccgc cctgacactg accgtgctga tgtgcagcgc ctcggtggcg ggattcacga
     2341 tgcgccccga caccacggcc gcgagcaagg gcaatgccgt ctcgctcgag agcttggtgc
     2401 ccaagcgctt cggtgactgg gtcgagttgc ccgagcaggg cgcgcaggtc gtcaatccgc
     2461 agacgcagga actgctggac aagctctaca gccagttgct ggtgcgcacc tatgtcaaca
     2521 aggagggcta ccgcatcatg ctgtcgatgg cctacggcga cgaccagcgc ggcggcttgc
     2581 aggcgcaccg cccggaggtg tgctaccccg cgcagggttt caagctgggc aaggtcgagg
     2641 acggcctgct tcccacctcg tacggcaaca tcgatgtgcg ccgcctcgcc acgagcctgg
     2701 gcgcgcgcaa cgaacccgtc acctactggc tgaccgtcgg cgatcaggtg atcaagaaca
     2761 cgctggacaa gcgcatcgcg gaaatccgcc tcggcctgac cggccagatc cccgacggcc
     2821 tgctgttccg gatctcgtcg atcgacaacg acaacgcgcg gggcttcgag atgcagcgca
     2881 agttcgccgc cgagatgatg tcggccgtgc cggccgcggc tcgcaagcaa ctcagcggcc
     2941 tggcgcccgc gcccgcggcg agctgaactc cgggctcgcg ccttcggcac cttgcgcaca
     3001 atgctcacgg gcctgtcctg tcagggcaac cacttgtcca gcagccagtc gagaaagaag
     3061 aagaacgcca gcgcgaccag catcagcacc atccccgcgg cgccgtgcag gaaaccctgg
     3121 ccggcctcgt cgcccaagta gtaggtgacc agcaccagcg tgatgacgcg cacgatgttc
     3181 gcggcgaacg cgatcggcag gatcgaggcg agcatgaccg cccgatgcgg ccagctcctg
     3241 cggcccatca ggtacatgaa cagcgtgccc agggccgcca ggctgaacat cgaatgcagg
     3301 ccggagcagg cgtcggccac ctgcagctga tactggccga tcgacaggat cacgccgctg
     3361 cgcgcgatcg ggtaacccac cgcgtacagc aggctctcga cgatgttggc gatctggctc
     3421 ttcagcggcc cggtgatcgc gtcgaccagg atgcccggca gcgggatcat gaagatgaag
     3481 tacagcagcg ggaaccacgc gaggcggatc gccgcgctgc cgcgcatcag cagcagcgcg
     3541 ccggcgacga ccagcggctg ggacgcgaac tcgagaatcg agatgtcgaa cacccggccg
     3601 acgaaataca cgaacaggcc gacgccgaac agcggccagc cgagcgccgg caccggcttg
     3661 acgggcgcct ggtcgatctc gtggcgcagc gtccagaaca accacagcat caccgccagg
     3721 atgatcgcgc cgtggccgtg ctcctcgctc ttccagatgc cgttggccgc ccaccagtag
     3781 agcggcaggt acatcgccgc gaagccggcg agcgcgacca gccaggccgt cggcagcttc
     3841 acgccggcct cgctgctgtt gtcgcgcagc acactgctca cgggcccttc tccttcttcg
     3901 gcttgccggc cggcacttcg ttcagcaccg agccgaccac gctcacgccg gcgttcatca
     3961 ggccatggac catgtcggtg aagcccgaca ccttggtctg cgagttgcgc gccacggcca
     4021 gcgcggcgcc ggtgcggacc gcgaccatcg cggcgtcttc gcccaccgac agcgccggcg
     4081 tgtcgatgat cacgatgtcg aaggtggcct tggcctgaat caggaactcg tcgaagttca
     4141 gccggttcag cagctcgagc ggattcggcg gcaccgggcc ggccggcagc acgcacagtc
     4201 ccgccagatc ggggatgcgc acgatcgctt cctcacgcga gcgccccgac agcacggtcg
     4261 agaagccgat cttgttctcg agatagaaga gctcgtgctg gcgcggatgg cgcagatcgg
     4321 cgtccaccag caaggtgcgc tcgccgagct gcgagaacac caccgccaga ttggccgcaa
     4381 ggtggctgcg gccttcgccg cgctccgcgc cgatgatcgt cagcacctgg cgctggtcgg
     4441 ccttgtcgaa ccagcgcagc atcagctggc tgcgcaccgc gcgcagttcc tcgacgcgcg
     4501 agctgaaggg ttcgtaggcc gccacgagct cgtcgctgac cggcttcttg tcgccgggcg
     4561 tcttgcgcag gtaggcgtaa gcgaactggc gcgacagcgc gtactggatg tccgcctcgg
     4621 tgagcaggcc caggcgcacc gcggcgtcgc cgaagcgcag gttgtgttcc ttctggacca
     4681 gcagcacgcg ctcggcgtcc tcgggcttga gcaggccgct gtcgatcagg atgccgccca
     4741 gcgagcggct gacccggggc gccccggaca acatcgacac ggtcatcttc atgaacgcac
     4801 tcccggcgcc gccagcaagg gccggcccgg tccggccggg cccacctgca acaggcggcg
     4861 aaacaccggg cgcttcgagc cttcggggcg cagcacgccg agcaccggca cgccggccat
     4921 caccatcagg tcctccggat cgcgtacgcg ccggtcgatc agttcccagc ccagcgccgc
     4981 cagcacgccc agcaccagcc cgccgatgat cgaacccgcg atgcccaccg ccaccttcgg
     5041 gcgcgaggcc tcgagcggct cgacggccgg gctcagcagc cgcgtgttgg cctggttgtt
     5101 ctggctttcg aggttgaatt gcccgacccg cgtgctgacg ccttcgtagg cgcgctgcgc
     5161 cgtgtcgaca tcgcgcttga tgacggcctc gaggtcgcgg tcggcgcgca gggtctgcag
     5221 cttcttcttc tgctcgtcga ccatcgcctg caattcggcg accttctgac cactggtgcg
     5281 gctgaccgtc gaggtaccgc ccgacacgcg ccgcgattcg gcggcgatct gcgcccgcag
     5341 ttcgttgagc tgcgcctcga ggcggatgcg ctcgggatga ttcttgcccc agatcgagct
     5401 catctcggac atgcgcgcct cgccggtcgc gagttgcgac ttcagcatct ggaccgcggg
     5461 gctcgacagg atgtcgggcg acgattgacc gcccgagttg cggttgcgcg tctcggcatc
     5521 gacccgctcg gcctgcgccg cgctcaactg cgccgtcagg gcctggtagc gggcattctc
     5581 ctgatcgaag cgttcgtccg agacgacgat gcccttgtcc tgctggaact tcgacaagcg
     5641 cgcctgcgcg gtctcgaggt tgttgcgcag caccttggtc tggtcgtcga ggaacgccgc
     5701 cgactggcgc gccggcgcca cgcgcagctc gaccgagatg tccatgtagg cctgcacgaa
     5761 cgcgttggcg gcggcctgcg cgaagatcgg atcctgcgcc gagaacgaca cgttgatgac
     5821 gttgctgccg cgcccgggct cgaccgccag accgcgctgg agcaggttcg cgaagtaacg
     5881 ttcgagcggg atcttcgcgt tggtctggtc acgccactgc gccaccgctg ccggcgagcg
     5941 ctcgacaccg agcatcttga cgacgcgcga cgcgacgcgt tcgctcttca atatgtcgac
     6001 ctgcgtcgcc agatagcccg gcaccgccag gcccggcgcg atcgcgccga gcaccgggtc
     6061 gatgcgcatc tcgacgatca tcgcggactc ggccgtgtac tgcttcggca gcaacagcgt
     6121 gatcgccgtc cccgcgaccg agatcacgac cagcagcgcc agtaccagcc atttgcgcgc
     6181 ccagacaacg cgcagtattt gtttgatcga catgatgatt cgctgacgcc ctcttagaag
     6241 agggattcct tgacgtagat cacatcgtcg cgcgcgaccg gatcgttcag gtcgaggctg
     6301 atgatctcga cggccccgtt cgcgtcgcgg cgatgcacct tgatcccgcg ctgcgtgccg
     6361 cgcgccgtga ggccgccgct ttgcgccagc gcctgcatca gcgtcatgcc gcgctccagc
     6421 ttgaactgcc cggggcgctg cacctcgcca tagatgtagg ccgtcggcgc gcggtcggcg
     6481 tagatgacgt cgccgttctg cacctcgagg tcgagctcgg ccttgcccga ctgcaggatc
     6541 gccggcaggt cgatgtcgag cttgatcggc ttgccgttgc gcgtgcccac cagcgtgacg
     6601 acgtcggcgc cgccgggaac gaccccgccc gccgcagcga ccatctccga caccttggcg
     6661 ccgaccgtct cgatcgggta gcggcccggc ttgccgacct ggccgaggat cgagatcacg
     6721 gcgctgcgaa tctgaccgac ctggatcgtg acctgcgggc gcagcacgaa gccgccgtcg
     6781 cgcagcagct tggcgatctt ctgttcggcc gcgctggcgg tcagcccgcc gatcacgacg
     6841 ttgcccacca gcgggaagtt gatctgattg gtctccgaaa tgcgcgcgcc atcgaccgtg
     6901 aggtccgggt tctggtacac ggtgatcttg accacgtcgc cggcacccag cagcgcctcg
     6961 gccttggccg cgggcgccgt ggccgacggg ctggtttggg cgaacgcgac gccgcagcac
     7021 aaggtcagcg ccagcagcac agagcgaaag aggttgggca ttttttgtct catgcttcgg
     7081 tgggtgtcgg ggcgccgggc gccgtatcga gctgaatcgt cacttgaatc ccttgatgcc
     7141 cttttcgagc gtgccggtgg tcggcgacga cgccggcgcc gtgttgatcg gctcgacctc
     7201 gacctgcggc gccgcgctcg ggatcgagcc ggccggcatc gccggcgcga ccgaggtcag
     7261 cggcggcgcc tccgacgcgg ccggcggggg cggcttcact tccttcgagc cgcccttggc
     7321 gtagtcgccc acgtactcga tcttggccgc gccgcgcagc gccttcacgt cgtcctcgat
     7381 caccttgcgc ttgcgctcgt tccacaggaa ctgctcgatc gccgggacgg catgcgcctc
     7441 atcgaccggc tggctgcgcg ctccggccat gatcaccacc tgcgccccgg tcggggttgc
     7501 gctgaagatc gtctggccgt ccttcagctg tgcgaacctg tccaggctcg ccagcggcaa
     7561 ttgctcggcg ccccgcaccg cctgattggc ggcgaacttg aaatcgttcg ccttcaggta
     7621 gttgacgaag tccggcacat ccttggacgc cccgagcttg gcctgcaact cggcgacctg
     7681 ctcgggcctg gcctggatcg ccagttcctg caggttgtag acgcggcgct ccttgaacag
     7741 cgccgggtgc gcctcgtagt aggccttgat ctcggccgcg ctgggcttgg gcgcgcccgc
     7801 ggcgatcttg tcgacgtagg ctcgcgcgat gatctcgcgg cgtgccgcct cgagctgctg
     7861 cacgacgcgc ggatcgcggt cgagcttctg gtcctgcgcc ttctgcagcg ccagttcctg
     7921 gtcgatcagc cgctccagcg ccaccttgct ggccgacgcc gcctgctcgg gtgccaggcc
     7981 acgctgctgc tgcagcacga agttgatctg gtgaacggtg atttcttcct tgttgacctt
     8041 ggccgcggtc tgcgaagcgg ccggtttgtc cttcttctcg ccacagccgg tgagtgccac
     8101 cgccagcacc gttgccgtca gggccgccag cgtcatcggt ccgacgcctc cccgcatggc
     8161 aggtgcatga attcttgaag tcattcgaac atcgctccca acgcatcaaa attgtggctc
     8221 agcgagcggc catacctccc gcagcggggc cggatccgcc cgtcggacgg cagcgcgcgt
     8281 caccgtagtc gcacgcctac accaaccctc gcggagtgta cttgtgaagc atgaccgcgc
     8341 agcaaccccc aactgaagga ccgccggcca caatctgtca cgaaatcgtg aacaagtcgc
     8401 gcgttggcac cggccagcgc agacttacag caaggcttgg t
//
LOCUS       DATMJB010000212         2812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_637908, whole genome shotgun sequence.
ACCESSION   DATMJB010000212 DATMJB010000000
VERSION     DATMJB010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637908"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VNU48_04700"
     CDS             <1..594
                     /locus_tag="VNU48_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWI10607.1"
                     /translation="SSAIPFVFPAVGLRIGDHREYCGDGSMRQAAPISPAVHLGGERI
                     LVVGAGRMHEPPGERVGVSEYPNLAQIAGHALSNIFLDALAVDVERLQRINSTLALLT
                     PQARAATSLKPIEVLVIAPSQRLDDIAAKHLGALPLPIRTMLRGIGVQGQGDDARGAA
                     LASYLLFESAYTRELMALGVRDTMQRRDDVIAFFGWA"
     gene            complement(650..1006)
                     /locus_tag="VNU48_04705"
     CDS             complement(650..1006)
                     /locus_tag="VNU48_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWI10608.1"
                     /translation="MNLVIKTIVGAAFALLAAGSFAAVDINRASQAELESIKGIGPSL
                     AQRILDARKSGTFKDWMDLQSRVKGVRAANSARFSADGLTVGGAAFNAPAVTAAPTRA
                     AKAPKAAKAGKAERVK"
     gene            1401..>2812
                     /locus_tag="VNU48_04710"
     CDS             1401..>2812
                     /locus_tag="VNU48_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10609.1"
                     /translation="MSSNAHHRPLRRLALALAAAGLLAASLFVVDRPSGGNTPAAATA
                     AGSTFDPLLDTGPASVCGPVAGTSMKIPAAWFIAAVAKTETTPFQPQPMKAAGGDVPL
                     YRELGALAFPITTRNARAQAYFNQGLRLSFAFNHAEAQRAFRVAQTLDPNCAACFWGE
                     ALILGPNINVPMMPEANAPALAALEKAVALKDRAGPKERALIEALEKRYSADPKAERP
                     ALDAAYADAMKQVAARFAADDVIRTLYVESAMDTQPWDYWEPGGAKAKGRGAEIVSNL
                     ETVLKRSPSHPGAIHLYIHAVEASTAPERALPYAERLAELMPGAGHIVHMPAHIYYRV
                     GQYRRSLDINKRAVAVDERYFQGSPSDPLYKSAYYPHNIHFVMVSAQMGGDGATAVAA
                     AKKLDAAVPTELAMQFQIMEPVKAAPYTTQLTFADPDTVLALPAPDEKLVLVRAMYHY
                     ARATAQALKKDTTAAQAEVDA"
BASE COUNT          412 a         1030 c          972 g          398 t
ORIGIN      
        1 tcgtcggcga tccccttcgt gtttcccgcg gtggggctgc gcatcggcga ccaccgcgag
       61 tactgcggcg acggctcgat gcgccaggcc gcgccgatct cgcccgcggt gcacctgggc
      121 ggcgaacgca tcctcgtcgt cggcgccggc cgcatgcacg agccgccggg cgagcgtgtc
      181 ggcgtcagcg agtacccgaa cctggcgcag atcgcgggcc atgcgctgtc caacatcttc
      241 ctggatgcgc tcgcggtcga tgtcgagcgc ctgcagcgca tcaacagcac gctggcattg
      301 ctgacgccgc aagcgcgcgc ggcgaccagc ctgaagccga tcgaggtgct ggtgatcgcg
      361 cccagccagc gcctggacga catcgccgcc aagcacctgg gcgcgctgcc gctgccgatc
      421 cgcacgatgc tgcgcggcat cggcgtgcaa gggcagggcg acgatgcgcg cggcgcggcg
      481 ctggcgagct acctgctgtt cgagtcggcc tacacgcgcg agctgatggc gctgggcgtg
      541 cgcgacacga tgcagcggcg cgacgacgtg atcgccttct tcggctgggc ctgacgcgcc
      601 ggtcgcatgc caacgccccg gcgcgccggg acgtgcgcgt ggaccgcggc tacttcacgc
      661 gctcggcctt gccggccttc gccgctttcg gtgccttcgc ggcgcgggtc ggagcggccg
      721 tgacggccgg ggcgttgaac gcggcgccgc cgaccgtcag gccgtcggcc gagaacctgg
      781 ccgagttggc ggcgcgcacg cccttcacgc gcgactgcag gtccatccag tccttgaacg
      841 tcccgctctt gcgcgcatcc aggatgcgct gcgccagcga cgggccgatg cccttgatgc
      901 tctcgagttc ggcctggctg gcccggttga tgtcgacggc ggcgaacgag ccggccgcga
      961 gcagcgcgaa ggcggcgccg acgatggtct tgatgacgag gttcatgggt cttctccggt
     1021 ggtttcgggc gctgcgggat tgcaggcccg gcccgaagtg ttcggccgcg gcgccggcgt
     1081 gaccatcgcc accacggggc gccccgcaag gtcgcccccg aaagcgggag ctgaaaatcg
     1141 aaccggcggg tgtggacccg tgacggtgtg tcggctggcg acccactgcg gtcataggcg
     1201 gcgacacggc cccgcccaaa ggcgccggtc gtggggtttg ccgaccctgt cggccgcttg
     1261 cgccgccgct ccgtcggtgt caggatcatt cccgctccgc gacccacgcg taacccgctg
     1321 acaggcgccc ggcgacggcc ccggtcctgc cgagctctgc ataagacggc cgtcgttcct
     1381 cgagaaggaa ggggcctgcc gtgtcgtcca acgcccatca ccgcccactg cgccgcttgg
     1441 cactcgcgct cgccgccgct ggcttgctgg ccgcgagcct gttcgtcgtc gaccgcccga
     1501 gcggcggcaa cacgccggcc gccgccacgg ccgccggatc cacgttcgat cccctgctcg
     1561 acaccggccc ggcctcggtg tgcggcccgg ttgccggcac gtcgatgaag attcctgccg
     1621 cctggttcat cgccgccgtg gccaagaccg agaccacgcc gttccagccg cagccgatga
     1681 aggccgccgg cggcgacgtg ccgctctacc gcgagctcgg cgcgctggcc ttcccgatca
     1741 cgacccgcaa cgcgcgcgca caggcctact tcaaccaagg gctgcggctg tcgttcgcct
     1801 tcaaccacgc cgaggcacag cgcgccttcc gggtggcgca gacgctcgat ccgaactgcg
     1861 cggcctgctt ctggggcgag gcgctgatcc tcgggcccaa catcaacgtg ccgatgatgc
     1921 cggaagcgaa tgcgccggcg ctcgccgcgc tcgagaaggc ggtggcgctc aaggatcgcg
     1981 caggcccgaa ggagcgcgcg ctgattgaag cgctcgagaa gcgctattcg gccgacccca
     2041 aggcggagcg cccggcgctc gatgcggcct atgccgatgc gatgaagcag gtggccgcgc
     2101 gcttcgcggc cgacgacgtg atccgcacgc tctacgtcga gagcgcgatg gatacgcagc
     2161 cgtgggacta ctgggagcct ggcggcgcca aagcgaaggg gcgcggcgca gagatcgtct
     2221 ccaacctcga gacggtgctc aagcgcagcc ccagtcatcc cggcgcgatc cacctataca
     2281 tccacgcggt cgaggcctcg accgcgccgg agcgggcgct gccatatgcc gagcgcctgg
     2341 ccgagctgat gcccggcgcc gggcacatcg tgcacatgcc ggcgcacatc tactaccggg
     2401 tgggccagta ccggcgctcg ctcgacatca acaaacgcgc ggtcgcggtc gacgagcgct
     2461 acttccaggg ctcgccctcc gacccgctct acaagagcgc ctactacccg cacaacatcc
     2521 acttcgtgat ggtctcggcg cagatgggcg gcgatggcgc gaccgccgtc gccgcggcga
     2581 agaagctcga tgcggcggtg cccaccgagc tggcaatgca gttccagatc atggagccgg
     2641 tgaaggccgc gccctacacc acgcagctca ccttcgccga ccccgacacc gttctggcgc
     2701 tgcccgcgcc cgacgagaag ctcgtgctgg tgcgggcgat gtaccactac gcccgggcca
     2761 cggcccaagc gctcaagaag gacacgacgg ccgcgcaagc cgaggtcgac gc
//
LOCUS       DATMJB010000213         3193 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_871560, whole genome shotgun sequence.
ACCESSION   DATMJB010000213 DATMJB010000000
VERSION     DATMJB010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871560"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VNU48_04715"
     CDS             <1..376
                     /locus_tag="VNU48_04715"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HWI10610.1"
                     /translation="AAPGPNLLFVAVLGWNIAGALGMLATMTGILVPSTVLSLWVTRW
                     GAQRRETRGVRAFTSGLTPLTLGLLVSTGWVLAEPYLRNPAHRWGALALIVVSVVAML
                     RTRLSPMWLVALGSAAGALGWV"
     gene            complement(383..910)
                     /locus_tag="VNU48_04720"
     CDS             complement(383..910)
                     /locus_tag="VNU48_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10611.1"
                     /translation="MKNFPFVQPLVQRWRESPSGVIAPTDVRRPFRLWMTGSLAVVMA
                     AMIPLRAASETERSHALRVLRHSRFGVSETLQRIEVAARDRGLSVLAMVPGAPPALVL
                     AASIGGTLVVMSEADSRPAMPMSMMVREAADGGADVLVAAAPEAGARHDWSELPRSVV
                     DELQALPDVVERALG"
     gene            complement(1005..1631)
                     /gene="upp"
                     /locus_tag="VNU48_04725"
     CDS             complement(1005..1631)
                     /gene="upp"
                     /locus_tag="VNU48_04725"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HWI10612.1"
                     /translation="MPVIDVKHPLVKHKVGLLRESEISTKKFRELTNELAWLLAYEAT
                     ADFPLETTTVQCWSGPTEVQRISGKKVTVVPILRAGLGMLDGVLDMIPSAKVSVVGLS
                     RNHETLQPENYFEKFVGQLDERTAIIIDPMLATAGSMIATVSLLKARGCKDIRALVLV
                     AAPEGVAALQRAHPEVRCWTASIDSHLNEVGYIIPGLGDAGDKIFGTK"
     gene            complement(1655..2389)
                     /gene="rlmB"
                     /locus_tag="VNU48_04730"
     CDS             complement(1655..2389)
                     /gene="rlmB"
                     /locus_tag="VNU48_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HWI10613.1"
                     /translation="MQVLFGFHAVTVRLKSAPGSVLEVHVDPARRDQRMRQFVERARD
                     ARVKLIDSDDERLGKLCGSHRHQGVAARVEAIALSHSLDDTLDAVAGPALLLVLDGIT
                     DPHNLGACLRVADGAGAHAVIAPKDHAVGLNATVAKVASGAADTVPYFMVTNLARTLN
                     ELKERDIRVIGTSDDAQRSLYELDLTGPVALVLGAEGSGMRQLTSRTCDELVRLPMAG
                     AVESLNVSVASGVCLYEAVRQRLKKD"
     gene            2434..>3193
                     /locus_tag="VNU48_04735"
     CDS             2434..>3193
                     /locus_tag="VNU48_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI10614.1"
                     /translation="MDSSSQRTECVVAGGGPAGLMLGYLLARAGVEVVVLEKHTDFLR
                     DFRGDTIHPSTLEVMHELGLLDAFLRLPHQRVERLAGQFGPARIVLADFAHLPTRAKF
                     IAMMPQWDFLDFLASEARRYPTFRLMMNAEATSLVERDGRVVGLRADTPEGGLEIAAD
                     LVVAADGRDSRLRAASGLDVVDFGAPMDALWFRLSRAAGDTDQTQGRFDAGRIFVMLN
                     RGDYWQCAFVIAKGSFARVREAGLDAFRRSLAPLL"
BASE COUNT          443 a         1141 c         1102 g          507 t
ORIGIN      
        1 ggccgcgccg gggccgaacc tgctgttcgt cgcagtgctg ggctggaaca tcgccggcgc
       61 gctcggcatg ttggcgacga tgaccggcat cctcgtgccc tcgaccgtgc tgtcgctgtg
      121 ggtgacgcgc tggggtgcgc agcgccgcga gacgcgtggt gtgcgcgcgt tcacctccgg
      181 cctgacaccg ttgacgctcg gcctgctcgt gtcgaccggc tgggtgctcg ccgagcccta
      241 tctgcgcaac ccggcgcatc gctggggcgc gctcgcgctg atcgtcgtca gcgtcgtcgc
      301 gatgctgcgc acgcgcctga gcccgatgtg gttggtcgcg ctgggcagcg cggccggcgc
      361 gctcggctgg gtctgaccgc gctcagccca gcgcgcgctc gacgacgtcg ggcagcgcct
      421 gcagttcgtc gaccacagac cgcggcagct cggaccagtc atgccgcgcc cccgcttccg
      481 gggcggccgc gaccagcacg tcggcgccgc cgtcggcggc ttcgcgcacc atcatgctca
      541 tcggcatcgc ggggcgcgaa tcggcctcgc tcatcaccac cagcgtgccg ccgatcgacg
      601 cggccagcac cagcgccggt ggtgcgcccg gcaccatcgc cagcaccgac aacccgcggt
      661 cgcgcgccgc gacctcgatg cgctgcaggg tctcgctgac gccgaagcgg ctgtggcgca
      721 gcacgcgcag cgcgtggctg cgctcggtct cggaggccgc acgcagcggg atcatcgcgg
      781 ccatcacgac cgcgaggctg ccggtcatcc acaaacgaaa cggacggcgc acgtcggtcg
      841 gcgcgatcac gccggacggc gactcgcgcc aacgttgcac cagcggctgg acgaacggga
      901 aattcttcat gccgcaagcg tagaagccgc gtgtgactgc agcaagacct atagcgtcaa
      961 gattggtaaa cgacgcggtt cgatcagcag gtgaaaacaa acatttactt cgtgccgaag
     1021 atcttgtccc cggcgtcgcc cagccccggg atgatgtagc cgacctcgtt cagatggctg
     1081 tcgatcgacg cggtccagca gcgcacttcg gggtgcgcgc gctgcagcgc agccacgccc
     1141 tcgggcgcgg cgaccagcac gagcgcgcgg atgtccttgc agccgcgcgc cttcagcagg
     1201 ctcacggtgg cgatcatcga gcccgccgtg gcgagcatcg ggtcgatgat gatcgcggtg
     1261 cgctcgtcga gctggccgac gaacttctcg aagtagttct cgggctgcag cgtctcgtgg
     1321 ttgcgcgaca gcccgacgac gctgaccttc gcgctcggga tcatgtcgag cacgccgtcg
     1381 agcatgccca ggccggcgcg caggatcggc accacggtga ccttcttgcc gctgatgcgc
     1441 tgcacctcgg tcgggccgct ccagcattga accgtcgtcg tctcgagcgg gaagtccgcg
     1501 gtcgcctcgt aggcgagcag ccacgcgagc tcgttcgtca gctcgcgaaa cttcttggtc
     1561 gagatctcgc tctcgcgcag caggccgacc ttgtgcttga ccagcgggtg cttgacgtcg
     1621 atgacgggca tgatgtcgaa ctccagtgct tgtttcagtc cttcttcagg cgctggcgca
     1681 ccgcttcgta caggcacacg ccgctggcga ccgacacgtt cagactctcg accgcgccgg
     1741 ccatgggcag gcgcacgagc tcgtcgcagg tccgagacgt gagctggcgc atgccgctgc
     1801 cttcggcgcc gagcaccagc gcgaccgggc cggtcaggtc cagctcgtag agcgagcgct
     1861 gcgcgtcgtc ggacgtgccg atgacgcgga tgtcgcgctc cttcagctcg ttcagcgtgc
     1921 gcgcgagatt cgtgaccatg aagtacggca cggtgtcggc cgcgccgctg gcgaccttcg
     1981 cgaccgtcgc gttcaggccc accgcatggt ccttcggtgc gatcaccgcg tgcgcgccgg
     2041 caccgtcggc cacgcgcaga caggcgccca ggttgtgcgg gtcggtgatg ccgtcgagca
     2101 ccagcagcag cgcggggccg gcgacggcgt cgagcgtgtc gtcgagcgaa tggctcagcg
     2161 caatcgcctc gacccgcgcc gccacgccct ggtggcggtg cgagccgcag agcttgccca
     2221 ggcgctcgtc gtcgctgtcg atcagcttca cgcgggcgtc gcgggcgcgc tcgacgaact
     2281 gccgcatgcg ctggtcgcgc cgcgccggat cgacgtgaac ctcgagcacc gaacccggcg
     2341 ccgatttcag gcgcaccgtg accgcgtgga agccgaacag gacctgcatg ccttttgcca
     2401 tgcggcgatg ttacggcgaa gtaccatcgc ggcatggaca gcagctcgca gcgcaccgaa
     2461 tgcgttgtcg ccggcggcgg cccggccggg ctgatgctgg gctatctgct ggcgcgcgcc
     2521 ggcgtcgagg tcgtcgtgct cgagaagcac accgacttcc tgcgcgactt ccgcggcgac
     2581 acgatccacc cctcgacgct ggaggtgatg cacgagctcg gcctgctcga cgcgtttctg
     2641 cgcctgccgc accagcgggt ggagcggctc gccggccagt tcggcccggc gcgcatcgtg
     2701 ctcgccgact tcgcgcatct gcccacgcgc gcgaagttca tcgcgatgat gccgcaatgg
     2761 gacttcctcg acttcctcgc cagcgaggcg cggcgctatc cgacgttccg gctgatgatg
     2821 aacgcggagg cgacgagtct cgtcgagcgc gacggccgcg tcgtcggcct gcgcgccgac
     2881 acccccgaag gcgggctcga gatcgccgcc gacctcgtgg tcgccgccga cggtcgggat
     2941 tcgcggctgc gcgctgcctc ggggctcgac gtcgtggact tcggcgcgcc gatggacgcc
     3001 ctgtggttcc gcctctcgcg cgcggccggc gacaccgacc agacgcaggg tcgcttcgac
     3061 gccggccgca tcttcgtgat gctgaaccgc ggcgactact ggcagtgcgc cttcgtcatc
     3121 gccaagggca gcttcgcgcg cgtgcgcgag gccgggctcg acgccttccg ccgctcgctc
     3181 gcgcccctgc tcc
//
LOCUS       DATMJB010000214         2301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_654346, whole genome shotgun sequence.
ACCESSION   DATMJB010000214 DATMJB010000000
VERSION     DATMJB010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654346"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VNU48_04740"
     CDS             complement(<1..520)
                     /locus_tag="VNU48_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWI10615.1"
                     /translation="MALQLLFHPFSSYCQKALVALYEYAIPFEPLLLEGPDSPASQRL
                     ATLWPMKRFPVLADGARTIIEASCIVEYLGLRMPAPLRLIPDDAPAALEVRMMDRFFD
                     NYVATPQQKIVSDALRPQADRDAYGVRQAREMLDTAYAWLDPLMARREWAAGDRFSLA
                     DCAAAPFLFYADW"
     gene            complement(541..>2301)
                     /gene="polA"
                     /locus_tag="VNU48_04745"
     CDS             complement(541..>2301)
                     /gene="polA"
                     /locus_tag="VNU48_04745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HWI10616.1"
                     /translation="WVAKVEAAALTAIDTETDSLDGMRAQIVGISLSTAVFEAAYIPL
                     RHGYAGAPDQLPVETVLAALRPWLENPAAPKLGQNIKYDTHVFANAGVNVQGYVHDTM
                     LQSYVLEAHKPHSLESLAERHLGRKGLSYEEMCGKGAHQIPFAQVAIEQAAEYACEDS
                     EMTLHVHQQLWPQIEAQPGLLDVYRRIEMPTSAILGRIERTGVLIDAALLAAQSQQLA
                     ERMVALEQEAYALAGQPFNMGSPKQIGEILFTKLGLPAKKKTASGAPSTDEEVLAELA
                     ADYPLPARLLEHRGLAKLKGTYTDKLPLMVNPATGRVHTNYAQAVAVTGRLASNDPNL
                     QNIPIRTPEGRRVREAFIPPPGHAILSADYSQIELRIMAHMSGDPGLLRAFEEGIDVH
                     RATASEVFGAALAEVSSEQRRYAKTINFGLIYGMGAFGLAQSLGIERSAAIAYIERYF
                     QRFAGVRRYMDETKASAHQKGYVETLFSRRIVLPEINGGSGPRKSAAERQAINAPMQG
                     TAADLIKLAMIAVQAAIDRERRATRMIMQVHDELVFDVPAAELDWARAEVPRIMASVA
                     ELKVPLLAEVGVGANWDQAH"
BASE COUNT          313 a          790 c          810 g          388 t
ORIGIN      
        1 tccagtcggc gtagaacagg aacggcgcag cggcgcagtc ggccagactg aagcggtcgc
       61 cggcggccca ttcccggcgt gccatcagcg ggtcgagcca ggcgtaggcg gtgtcgagca
      121 tctcacgcgc ctgccgcacg ccgtaggcgt cgcgatcggc ctgcggccgc agcgcgtcgg
      181 acacgatctt ctgctgcggc gtggcgacat agttgtcgaa gaagcggtcc atcatccgca
      241 cctcgagcgc ggccggcgcg tcgtcgggga tcaggcgcag cggcgccggc atgcgcaggc
      301 cgaggtactc gacgatgcag ctcgcctcga tgatggtgcg cgcgccgtcc gccagcaccg
      361 gaaagcgctt catcggccac agcgtggcca gccgctgcga cgccggactg tccggcccct
      421 cgagcagcag cggctcgaac gggatcgcgt attcgtacag cgcgacgagc gccttctggc
      481 agtacgacga gaacgggtga aagagcagct gcaatgccat gcgtgaaact ccttcggctt
      541 tcagtgcgcc tggtcccagt tcgcgcccac gcccacctcg gccagcagcg gcaccttcag
      601 ctcggcgacc gaggccatga tgcgcggcac ctcggcgcgc gcccagtcga gctcggcggc
      661 cggcacgtcg aacaccagct cgtcgtgcac ctgcatgatc atgcgggtcg cgcgccgctc
      721 gcggtcgatc gcagcctgca ccgcgatcat cgcgagcttg atcaggtcgg ccgcggtacc
      781 ctgcatcggc gcgttgatcg cctggcgctc ggccgcgctc ttgcgcgggc cgctgccgcc
      841 gttgatctcg ggcagcacga tgcgccggct gaacagcgtc tcgacatagc ccttctggtg
      901 cgccgaggcc ttggtctcgt ccatgtagcg gcgcacgccg gcaaagcgct ggaagtagcg
      961 ttcgatgtag gcgatcgcag cgctgcgctc gatgcccagg ctttgcgcca ggccgaacgc
     1021 gcccatgccg tagatcagcc cgaagttgat cgtcttggcg tagcggcgct gctcgctgct
     1081 gacctcggcc agcgccgcgc cgaagacctc gctggcggtc gcgcggtgca cgtcgatgcc
     1141 ctcctcgaac gcgcgcagca ggcccgggtc gccgctcatg tgcgccatga tgcgcagctc
     1201 gatctgcgag tagtcggccg acaggatggc gtgaccgggc ggcgggatga aggcctcgcg
     1261 cacgcgccgg ccctcgggcg tgcggatcgg gatgttctgc aggttcgggt cgttgctggc
     1321 cagccgcccg gtcaccgcca ccgcctgcgc gtagttggtg tgcacgcggc cggtcgccgg
     1381 gttgaccatc agcggcagct tgtcggtgta ggtgcccttc agcttggcca ggccgcggtg
     1441 ctcgagcagc cgggccggca gcgggtagtc ggcggcgagt tcggccagca cctcttcgtc
     1501 ggtgctgggc gcaccgctcg cggtcttctt cttcgccggc aggcccagct tggtgaacag
     1561 gatctcgccg atctgcttcg ggctgcccat gttgaagggc tggccggcga gcgcgtaggc
     1621 ctcctgttcg agcgcgacca tgcgttcggc gagctgctgg ctctgcgcgg ccagcagcgc
     1681 ggcgtcgatc agcacgccgg tgcgctcgat gcggccgagg atcgcgctgg tcggcatctc
     1741 gatgcgccgg tacacgtcga gcagcccggg ctgcgcctcg atctgcggcc acagctgctg
     1801 gtgcacgtgc agcgtcatct cgctgtcttc gcacgcatac tcggcggcct gctcgatcgc
     1861 gacctgcgcg aacggaatct gatgcgcgcc cttgccgcac atctcttcgt agctcaggcc
     1921 cttgcggccg aggtggcgct cggccaggct ttcgaggctg tgcggcttgt gcgcctcgag
     1981 cacgtagctc tgcagcatcg tgtcgtgcac atagccctgg acgttgacgc cggcgttcgc
     2041 gaacacatgc gtgtcgtact tgatgttctg cccgagcttg ggcgcggccg ggttctcgag
     2101 ccagggccgc agcgcggcca gcaccgtctc gaccggcagc tggtcgggcg caccggcgta
     2161 gccgtggcgc agcgggatgt aggcggcttc aaagacggcc gtcgacagcg agatgccgac
     2221 gatctgtgcg cgcatgccgt cgagcgaatc ggtctcggtg tcgatcgcgg tcagcgctgc
     2281 ggcctcgacc ttcgcgaccc a
//
LOCUS       DATMJB010000215         4827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_91190, whole genome shotgun sequence.
ACCESSION   DATMJB010000215 DATMJB010000000
VERSION     DATMJB010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91190"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VNU48_04750"
     CDS             complement(<1..273)
                     /locus_tag="VNU48_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HWI10617.1"
                     /translation="MFTFTPGTAPLLVSFPHAGTEIPDAIAARMTQEALQRADVDWHL
                     PQLYAFVRAMGASTIAARFARHVIDLNRPPEDTSLYPGQDVTGLLPL"
     gene            371..1333
                     /locus_tag="VNU48_04755"
     CDS             371..1333
                     /locus_tag="VNU48_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI10618.1"
                     /translation="MSSMHRVATIVAAIAAGALAAPALAQDTKVAIAISGWTGFAPLT
                     LASEAGLFKKNGLDVTIKKIPQKDRHLAIASGDVQCAATTVETWVVWNANGVPTTQIF
                     QLDKSYGADGMVVKPGVAKIADLKGKTVAASAPGTSPYFGLAWMLKKNGLSVKDVKVL
                     TLEPQAVANALIAGSTDFDAGMTYEPYLSAVRAKPEAGKIIATTLDYPMVMDTFGCAP
                     KFLKDNPKAAKALADSYFDALEMIKKEPKKSYEIMGADVKQTGEAFEKSAAYLRWQDR
                     AANQKFFAGEHAQFTKEAADLLLEIGTIKQIPELKTLADTSFIK"
     gene            1354..2127
                     /locus_tag="VNU48_04760"
     CDS             1354..2127
                     /locus_tag="VNU48_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI10619.1"
                     /translation="MKPLREIGPGRRQLLGLAFFALFLAAWAVATLGGFVPKTFLADP
                     IAMVKSGWLLLTQHGFAKDIGFTIWRVLGGFVLAALLAVPLGIAMGAYKPVEAFFEPF
                     VSFARYLPASAFIPLLILWAGIGEAQKLALIFIGSFFQLVLMIGSQVGGTRRDLVEAA
                     YTLGASDIGIVKRVLIPAAAPQIAETLRLVLGWAWTYVIVAELIGASEGIGHMITDSQ
                     ALLATDQIIFGIVTIGAIGLISDLAFKRANRAMFKWAAI"
     gene            2129..2917
                     /locus_tag="VNU48_04765"
     CDS             2129..2917
                     /locus_tag="VNU48_04765"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI10620.1"
                     /translation="MSTLSIEGVGRTFAGVNGGAPTLALQPTTLAVAHNDFITILGPS
                     GCGKSTLLRIVAGLDTPTVGRVRLDGREVTGPGADRGMVFQSYTLFPWLTVRQNICFG
                     LREKGLAAGEQAQIAARFIAEVGLAGFESHFPKQLSGGMQQRVALARALANDPKILLL
                     DEPFGALDNQTRALMQELLLSIWELHKTTVLFVTHDIDEAIFMANRCAVFSARPGRVK
                     NELQIDLPYPRHYTVKTTPRFSAFKAQVTEDIRVETLRAVQMEK"
     gene            complement(2924..4432)
                     /gene="hutH"
                     /locus_tag="VNU48_04770"
     CDS             complement(2924..4432)
                     /gene="hutH"
                     /locus_tag="VNU48_04770"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HWI10621.1"
                     /translation="MDTPHHTIAPGALDDALLVHAAAGGAHWAIADAARPGIAASVQA
                     VRDLVAQHAAVYGINTGFGKLAQTRIADADLAQLQTNLVLSHSAGVGAPLGRPVVRLM
                     LLLKAASLAHGVSGVRMEVIAALLALLNADVLPVVPEKGSVGASGDLAPLAHLAGVLI
                     GVGEAFVGDARVPAAQALAHAGLVPLQLGPKEGLALLNGTQASTALAIHAVQRLRRVF
                     DAAVAIGAMTVDAAKGSDTPFDPRIHAVRGQHGQRVVAARYRDWLARSALRASHLDCD
                     RVQDPYCLRCQPQVMGACLDQIEHAWGILLREANGVSDNPLVFADASGKDHRALSGGN
                     FHAEPVAFAADNLALAIAEIGALAERRTAMLMDPALSGLPAFLIEHGGLNSGFMIAQV
                     TAAALASENKSLAHPASVDSLPTSANQEDHVSMATFAARRLHAMLDNTAAVVGIEWLC
                     AAQAIEFHRPLRSSDAIEALLARLRAQVARLEVDRAMAPDIAAATALVEAGL"
     gene            4491..>4827
                     /locus_tag="VNU48_04775"
     CDS             4491..>4827
                     /locus_tag="VNU48_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWI10622.1"
                     /translation="MKKSPASTRTLPYATVKAALRERIAEGGWEPGVRLPSERELVQE
                     FGCARMTVHRALRELEEEGLIERRQGSGSYVAELHPISNLLQVRDIHDEIRERGHAHA
                     TRICQVARTR"
BASE COUNT          776 a         1693 c         1656 g          702 t
ORIGIN      
        1 gagcggcagc aggccggtca cgtcctgacc cgggtagagg ctggtgtcct ccggcggccg
       61 gtttaggtcg atcacgtggc gggcgaaacg cgccgcgatc gtgctcgcgc ccatcgcgcg
      121 gacgaaggca tagagctgcg gcagatgcca gtcgacatcg gcgcgctgca gcgcctcttg
      181 tgtcatgcgc gcggcgattg cgtcggggat ctcggtaccg gcgtgcggga acgaaacgag
      241 cagcggcgcg gtgcctggcg tgaaggtgaa catgcgcgaa gtggtctaga ccggttgagc
      301 gccgagtgta gactgcgcgg cacgcggctt gcgttcggcc gctcgcctct gccaaccccc
      361 gggagacacg atgagttcca tgcaccgcgt tgccacgatc gtcgccgcca tcgccgccgg
      421 agcgctcgcc gcgccggccc tggcccagga caccaaggtc gcgatcgcca tctccggctg
      481 gaccggcttc gcgccgctga ccctggccag cgaggccggc ctgttcaaga agaacggcct
      541 ggacgtgacg atcaagaaga tcccgcagaa ggaccgccac ctcgcgatcg cctccggcga
      601 cgtgcagtgc gccgccacca ccgtcgagac ctgggtcgtg tggaacgcca acggcgtgcc
      661 gacgacgcag atcttccagc tcgacaagag ctacggcgcc gacggcatgg tcgtcaagcc
      721 gggcgtcgcg aagatcgccg acctgaaggg caagacggtc gcggcctcgg cgccgggcac
      781 ctcgccctac ttcggcctgg cgtggatgct gaagaagaac ggcctgtcgg tgaaggacgt
      841 gaaggtgttg acgctcgagc cgcaggccgt tgccaacgca ttgatcgccg gcagcaccga
      901 cttcgacgcc ggcatgacct acgagcccta cctcagcgcg gtgcgcgcca agcccgaggc
      961 cggcaagatc atcgccacga cgctcgacta cccgatggtg atggacacct tcggctgcgc
     1021 gccgaagttc ctgaaggaca acccgaaggc cgcgaaggcg ctggccgaca gctacttcga
     1081 cgcgctcgag atgatcaaga aggagccgaa gaagtcctac gagatcatgg gcgcggacgt
     1141 caagcagacc ggcgaggcgt tcgagaagtc ggccgcctac ctgcgctggc aggaccgtgc
     1201 cgcgaaccag aaattcttcg ccggcgaaca cgcgcagttc accaaggaag cagccgacct
     1261 gctgctggag atcggcacga tcaagcagat ccccgagctg aagacgctgg ccgacacgag
     1321 cttcatcaag tagttgacgt tgaaggcgcg cgcttgaagc cgcttcgcga gatcggcccc
     1381 ggccggcgcc agttgctggg gctcgcgttc ttcgcgctgt tcctcgcggc gtgggccgtt
     1441 gccactctcg gcggcttcgt gcccaagacc tttctcgcgg acccgatcgc aatggtgaag
     1501 tcgggctggc tgctgctgac ccagcacggc ttcgcgaagg acatcggctt cacgatctgg
     1561 cgcgtgctcg gcggcttcgt gctcgcggcg ctgctggcgg tgccgctggg catcgcgatg
     1621 ggtgcataca agccggtcga ggccttcttc gagcccttcg tctcgttcgc gcgctacctg
     1681 ccggcctcgg cgttcatccc gctgctgatc ctgtgggccg gcatcggcga ggcgcagaag
     1741 ctcgcgctga tcttcatcgg ctcgttcttc cagctcgtgt tgatgatcgg cagccaagtc
     1801 ggcggcacgc gacgcgatct ggtcgaggcc gcgtacaccc tgggcgcgag cgacatcggt
     1861 atcgtcaagc gcgtgctgat cccggccgcg gcgccgcaga tcgccgagac gctgcgcctc
     1921 gtgctcggct gggcctggac ctacgtgatc gtcgccgagc tgatcggcgc cagcgaaggg
     1981 atcggccaca tgatcaccga cagccaggcg ctgctcgcga ccgaccagat catcttcggc
     2041 atcgtcacga tcggcgcgat cgggctgatc tcggacctgg cgttcaagcg cgcgaaccgg
     2101 gcgatgttca agtgggcggc gatctgacgt gagcacgctg tcgatcgagg gtgtgggtcg
     2161 caccttcgcg ggcgtgaacg gcggcgcgcc gaccttggcg ctgcagccga cgacgctggc
     2221 cgtcgcgcac aacgacttca tcacgatcct cggcccctcg ggttgcggca agtcgaccct
     2281 gctgcgcatc gtcgcggggc tggacacgcc gaccgtgggc cgggtgcggc tcgacggcag
     2341 ggaggtcacc ggccccggcg ccgaccgcgg catggtgttc cagagctaca cgctgttccc
     2401 gtggctgacg gtgcgccaga acatctgctt cggcctgcgc gagaagggcc tggccgcggg
     2461 tgagcaggca cagatcgcgg cgcgcttcat cgccgaggtc ggcctcgccg gcttcgagtc
     2521 gcactttccg aagcagctgt cgggcggcat gcagcagcgc gtcgcgctcg cgcgcgcgct
     2581 ggccaacgac ccgaagatcc tgctgctcga cgaacccttc ggcgccctgg acaaccagac
     2641 ccgcgcgctg atgcaggagc tgctgctgtc gatctgggag ctgcacaaga caaccgtgct
     2701 gttcgtgacg cacgacatcg acgaggcgat cttcatggcg aaccgctgcg cggtgttcag
     2761 cgcgcgcccg ggccgcgtca agaacgagct gcagatcgac ctgccgtacc cgcgccacta
     2821 caccgtcaag accacgccgc gcttctcggc attcaaggcc caggtcaccg aggacatccg
     2881 cgtcgagacg ctgcgcgcgg tgcagatgga aaagtgatgg agcctacagg ccggcctcga
     2941 ccagcgcggt cgccgcggcg atgtcgggcg ccatcgcccg gtcgacctcg agccgtgcga
     3001 cctgcgcgcg cagccgcgcc agcagcgcct cgatcgcgtc ggacgagcgc agcggccggt
     3061 ggaactcgat cgcctgcgcc gcacacagcc actcgatgcc gaccaccgcc gcggtgttgt
     3121 ccagcatcgc gtgcaggcgg cgcgccgcga aggtcgccat cgagacgtgg tcctcctgat
     3181 tcgccgaggt cggcagcgag tcgacgctcg ccggatgcgc gagcgacttg ttctccgatg
     3241 cgagcgccgc cgccgtcacc tgcgcgatca tgaagcccga gttcagcccg ccgtgctcga
     3301 tcaggaacgc cggcagtccc gacagcgccg ggtccatcag catcgcggtg cggcgctcgg
     3361 cgagtgcgcc gatctcggcg atcgccagcg ccaggttgtc ggccgcgaac gcgaccggct
     3421 cggcgtggaa gttgccgccg gagagcgccc gatgatcctt ccccgacgcg tcggcgaaca
     3481 ccaatgggtt gtcggagacg ccgttcgcct cgcgcagcag gatgccccag gcgtgctcga
     3541 tctggtccag gcacgcgccc atcacctgcg gctggcagcg caggcagtac gggtcctgga
     3601 cacggtcgca gtccagatgc gacgcgcgca gcgcgctgcg ggcgagccag tcgcgatagc
     3661 gtgccgcgac gacgcgctgg ccgtgctggc cgcgcaccgc gtggatgcgc ggatcgaacg
     3721 gcgtgtcgga gcctttcgcg gcatcgacgg tcatcgcacc gatcgcgacc gcggcgtcga
     3781 acacgcgccg cagccgctgc accgcatgga tcgccagcgc ggtcgaggcc tgcgtgccgt
     3841 tcagcagcgc cagaccttcc ttcggcccga gctgcagcgg cacaagaccg gcgtgcgcga
     3901 gcgcctgcgc ggccggcacg cgcgcgtcac cgacgaaggc ctcgccgacg ccgatcagca
     3961 caccggccag gtgcgcgagc ggcgccagat cgcccgacgc gccgaccgag cccttctcgg
     4021 gcacgaccgg caacacgtcg gcgttcagca gcgcgagcag cgcggcgatg acctccatgc
     4081 gcacgcccga cacgccatgc gccaggctcg ccgccttcag cagcagcatc agccgcacca
     4141 ccgggcgacc gagcggcgcg ccgacgccgg cgctgtgcga cagcaccagg ttcgtctgca
     4201 gctgcgcaag gtcggcgtcg gcaatacgcg tctgcgcgag cttgccgaag ccggtgttga
     4261 tgccgtagac cgcggcgtgc tgcgcgacca gatcgcgcac tgcctgcacg ctcgccgcga
     4321 tgcccggccg ggcggcgtcg gcgatcgccc agtgcgcgcc gccggccgcg gcatggacca
     4381 gcaacgcgtc gtcgagcgcg cccggagcga tggtgtggtg cggcgtgtcc atgcgtcgaa
     4441 gcctcgagtg gtctagacaa ctttagcatg cacgtcgcta gaatcgctcg atgaaaaaat
     4501 cgcccgcgtc gacccgcacg ctgccctacg ccaccgtcaa ggccgcgctg cgcgagcgca
     4561 tcgccgaagg cggctgggag cccggcgtgc gcctgccctc ggagcgcgaa ctcgtgcagg
     4621 aattcggctg cgcccgcatg accgtgcacc gcgcgctgcg cgaactcgaa gaggaaggcc
     4681 tgatcgaacg ccgccagggc tcgggttcgt acgtcgccga gctccatccg atctcgaacc
     4741 tgctgcaggt ccgcgacatc cacgacgaga tccgcgaacg cggccacgcg cacgcgacgc
     4801 gcatctgcca ggttgcccgc acccgct
//
LOCUS       DATMJB010000216         2120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_368606, whole genome shotgun sequence.
ACCESSION   DATMJB010000216 DATMJB010000000
VERSION     DATMJB010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368606"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2025
                     /gene="pnp"
                     /locus_tag="VNU48_04780"
     CDS             <1..2025
                     /gene="pnp"
                     /locus_tag="VNU48_04780"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWI10623.1"
                     /translation="GAVLVTVADTVVLATVVASHSAKPGQDFFPLTVDYIEKTYAAGK
                     IPGSFFKREAKPSEHETLTSRLIDRPIRPLFPEGFYNEVQVVIHVLSLNPEVSADIAA
                     LIGSSAALAISGIPFNGPIGAARVGYINGQYVLNPGKTQLLESKMDLVVAGTEAAVLM
                     VESEAQQLSEEIMLGGVVFGHEQGNIAISAINDLVRDAGKPAWDWVAAPKDEPFIAKV
                     VALAEGPLREAYQIRSKQARTQATRAVTAATVASLQGEGVAFDKVALDNVLFDIEARI
                     VRSQILSGEPRIDGRDTRTVRQIEIRNSVLPRAHGSALFTRGETQALVAATLGTARDA
                     QRIDALAGEFTEHFMLHYNMPPFATGETGRVGTPKRREIGHGRLAKRALVAVLPSQAD
                     FPYSMRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGNKFAVL
                     TDILGDEDHLGDMDFKVAGTAQGVTALQMDIKIQGITKEIMQVALAQAKEARLHILGK
                     MVEAMGGAKTEVSQFAPRLYTMKINPEKIRDVIGKGGATIRALTEETGTTIDIGEDGT
                     ITIASADADKAAYAKKRIEEITAEVEVGKVYEGPITKILDFGALVNLMPGKDGLLHIS
                     QIAHQRVEKVTDFLKEGQIVKVKVLETDEKGRVKLSMKALLEREPRPVQE"
BASE COUNT          400 a          710 c          713 g          297 t
ORIGIN      
        1 ggcgcggtgc tggttaccgt cgccgatacc gtcgtcctcg cgaccgtagt cgcctcgcat
       61 tcggccaagc cggggcagga cttcttcccg ctgacggtcg actacatcga gaagacgtat
      121 gccgccggca agatccccgg cagcttcttc aagcgcgagg ccaagccgag cgagcacgag
      181 acgctgacca gccgcctgat cgaccgcccg atccgtccgc tgttccccga gggcttctac
      241 aacgaagtcc aggtcgtgat ccacgtgctg tcgctgaacc cggaagtctc ggccgacatc
      301 gccgcgctga tcggttcctc ggccgcactg gcgatctcgg gcatcccgtt caacggcccg
      361 atcggcgccg cgcgcgtggg ctacatcaac ggccagtacg tgctgaaccc gggcaagacg
      421 cagctgctcg aatcgaagat ggacctggtc gtcgccggca ccgaggcggc cgtgctgatg
      481 gtcgagtccg aagcgcagca gctctcggaa gagatcatgc tgggcggcgt cgtgttcggc
      541 cacgagcagg gcaacatcgc gatcagcgcg atcaacgatc tggtgcgtga cgccggcaag
      601 ccggcctggg actgggtcgc cgcgccgaag gacgaaccct tcatcgccaa ggtcgtggcc
      661 ctcgccgaag gcccgctgcg cgaggcctat cagatccgct cgaagcaggc ccgcacgcaa
      721 gccacccgcg ccgtgacggc cgcgaccgtc gcgtcgttgc aaggcgaggg cgtggccttc
      781 gacaaggtcg cgctcgacaa cgtgctgttc gacatcgaag cgcgcatcgt gcgcagccag
      841 atcctgtcgg gcgagccgcg catcgacggc cgcgacacgc gcaccgtgcg ccagatcgag
      901 atccgcaaca gcgtgctgcc gcgcgctcac ggctcggcgc tgttcacgcg tggcgaaacg
      961 caagcgctgg tggccgccac gctcggtacc gcgcgcgatg cgcagcgcat cgacgcgctg
     1021 gccggcgagt tcaccgagca cttcatgctc cactacaaca tgccgccgtt cgcgaccggc
     1081 gagaccgggc gcgtgggcac gccgaagcgc cgcgagatcg gccacggtcg cctggccaag
     1141 cgcgcgctgg tcgcggtgct gccgagccag gccgacttcc cgtattcgat gcgcgtggtc
     1201 tccgagatca ccgagtcgaa cggttcgtcg tcgatggcct cggtgtgcgg cggctgcctc
     1261 gcgctgatgg acgcgggcgt gccgatgaag gcgcacgtcg cgggcatcgc gatgggcctg
     1321 atcctggaag gcaacaagtt cgcggttctg accgacatcc tcggcgacga ggatcacctc
     1381 ggcgacatgg acttcaaggt ggccggcacg gcgcaaggcg tgaccgcgct gcagatggac
     1441 atcaagatcc agggcatcac gaaggaaatc atgcaggtcg cgctggcgca ggccaaggaa
     1501 gcgcgtctgc acatcctcgg caagatggtc gaggcgatgg gcggcgcgaa gaccgaggtc
     1561 tcgcagttcg caccgcgcct ctacacgatg aagatcaatc cggagaagat ccgcgacgtg
     1621 atcggcaagg gcggcgcgac gatccgcgcg ctgaccgagg agaccggcac gacgatcgac
     1681 atcggcgaag acggcacgat cacgatcgcc tcggccgacg ccgacaaggc ggcctatgcg
     1741 aagaagcgca tcgaggagat caccgccgaa gtcgaagtcg gcaaggtcta cgaaggcccg
     1801 atcaccaaga tcctcgactt cggcgcgctg gtgaacctca tgcccggcaa ggacggcctg
     1861 ctgcacatca gccagatcgc gcaccagcgc gtcgagaagg tcaccgactt cctgaaggaa
     1921 ggccagatcg tcaaggtcaa ggtgctcgag accgacgaga agggccgcgt caagctgtcg
     1981 atgaaggcgc tgctggagcg tgagccgcgt ccggtgcagg aataactcga cgccgcgcca
     2041 gcgatgaaag cgatcgagat cacgagctac ggcgcccccg aggtgctcaa gccgacgcag
     2101 cgcgacgacc ccgtcgccgc
//
LOCUS       DATMJB010000217         4080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_361827, whole genome shotgun sequence.
ACCESSION   DATMJB010000217 DATMJB010000000
VERSION     DATMJB010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361827"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /gene="mtnN"
                     /locus_tag="VNU48_04785"
     CDS             <1..426
                     /gene="mtnN"
                     /locus_tag="VNU48_04785"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HWI10624.1"
                     /translation="PLTGRSHFDADAALAQTLGAAAQRCVDRADALVGAAHLRDFGIA
                     AARVHRGLIVSGDRFVASAAHSAELRAALPGALAVEMEGAAVAQACADFGRPFAVLRT
                     VSDRADDSAHVDFTRFIAEVASIYTRAIVEDWLQHGATP"
     gene            423..914
                     /locus_tag="VNU48_04790"
     CDS             423..914
                     /locus_tag="VNU48_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10625.1"
                     /translation="MSVLGRLWRVLRAMLLALAALWFFLEEFGWHPLAAWLRRFALWP
                     PWARLEGRIAAAPPRLALMLFLLPAGLLLPVKLLALALISDGHAMSGLAVIVAAKLVG
                     TAVGGWLFLLTRPQLMQMPRFARAMAWWRRLRWQVRKALNASAAWRALRQTLQRWRRR
                     RDR"
     gene            929..1651
                     /locus_tag="VNU48_04795"
     CDS             929..1651
                     /locus_tag="VNU48_04795"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HWI10626.1"
                     /translation="MPPRLFIDRPLTAGATFALPAGPARHAQVLRLQPGAALTLFDGT
                     GGESAATVVQMGRSEVTVRLGERTEVDRELPFQVTLALGMPANERMDTLVEKATELGA
                     STIQPLLCERSVLRLTGERADKKVAHWQAVALAACEQSGRTRVPVIAPVRALAQWLRE
                     LPLDARSRWMLGFRDARPVAALTMAPAGVVCLSGPEGGLSSDEEAAARAAGFEAVSLG
                     PRVLRAETAPLALLAFLALCAG"
     gene            1703..2176
                     /locus_tag="VNU48_04800"
     CDS             1703..2176
                     /locus_tag="VNU48_04800"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HWI10627.1"
                     /translation="MGSDDMGLLDSVLGALANSQPGAQPPEPGATQGVPGSVLLSTVI
                     AMLANGQQAQGAGVGGLGDLIGRFTQGGLGDVIGSWIGHGQNLPVSGDQLSQVLGPDV
                     IGKIAAQLGVTHGEAATQLSQVLPEAVDRLTPQGQAPSGGLGEIGDLLGQLTARR"
     gene            complement(2186..3421)
                     /locus_tag="VNU48_04805"
     CDS             complement(2186..3421)
                     /locus_tag="VNU48_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HWI10628.1"
                     /translation="MALPLFLPCAAGVEALLAEEVQRVLPKARVAEARGGVALEGDPL
                     EVMILNLESRLAQRVLIEVAHGSYRDEGDVYELARSVDWTQWITPQHTLRVDTTAQRS
                     PLRSLNFAALRVKDAVCDVLRDASGERPSVDTRHPDLQLVLHLGEREATVYVDSSGES
                     LFKRGWRNHEADKGDAPLKETLAAAMLAAAGWRGTADAGGALHDPCCGSGTIAIEAAQ
                     IACGIAPGLKRRFAFERLLPFVTDALRAELQRLRSHAQARIHPSPVPIHASDVSFRMV
                     DFARRNAQRAGVEAAITFNGGDALERPAPELPDGLPGTLMINPPYGERIEVGGKAARA
                     SAQDRDTPGDFFPRLASHWKRAYTQHPGGWTAYVLSPDMKLPSAMRLKESRRVPMWNG
                     PIECRLFRFDLVPGSARKG"
     gene            complement(3426..>4080)
                     /locus_tag="VNU48_04810"
     CDS             complement(3426..>4080)
                     /locus_tag="VNU48_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HWI10629.1"
                     /translation="RFVDVERNVVIPDVIVTRIGHGTSARFRVMLNPEVMPRLRVHDI
                     YAGALKAHKGEGSQALSQRLQEARWFIKNIQQRFDTILRVSNAIVERQKNYFVHGELA
                     MRPLVLREIADELGLHESTISRVTTAKYMNTPFGTFELKYFFGSSLNTEAGGNASSTA
                     VRALIKQIVAAENPAKPLSDSQISQMLDEQGIQVARRTVAKYRDALKIAPTQLRKAM"
BASE COUNT          508 a         1442 c         1504 g          626 t
ORIGIN      
        1 ccgctgaccg gccgttcgca tttcgatgcc gatgccgcac tggcccagac gctgggcgcg
       61 gcggcgcaac gctgtgtcga ccgcgccgac gcgctggtcg gtgcggcaca cctgcgcgac
      121 ttcggcatcg ccgccgcgcg cgtgcaccgc gggctgatcg tcagcggcga ccgcttcgtc
      181 gccagtgccg cgcacagcgc cgaattgcgc gcggcgctgc ccggcgcgct cgccgtcgag
      241 atggaaggcg ccgcggtcgc gcaggcctgt gccgacttcg gtcggccgtt cgcggtgctg
      301 cgcaccgtct ccgaccgtgc agacgacagc gcccatgtcg acttcacgcg cttcatcgcc
      361 gaggtcgcga gcatctacac ccgcgcgatc gtcgaggact ggctgcagca cggcgccacg
      421 ccatgagcgt gctcggccgg ctgtggcgcg tgctgcgcgc aatgctgctc gcgctcgcgg
      481 cgctctggtt ctttctcgag gaattcggct ggcacccgct cgccgcgtgg ctgcggcgct
      541 ttgcgctgtg gccaccctgg gcgcggctcg aagggcgcat cgcggccgcg ccgccgcggc
      601 tcgcgctgat gctgttcctg ctgcccgcgg gcttgctgct gccggtcaag ctgctggcgc
      661 tggcgctgat cagcgacggt cacgccatga gtgggctggc ggtcatcgtc gccgccaaac
      721 tcgtcggcac cgcggtcggc ggctggctgt tcctgctgac ccggccgcag ctgatgcaga
      781 tgccgcggtt cgcgcgcgcg atggcctggt ggcgtcgcct gcgctggcag gtgcgcaagg
      841 ccttgaacgc gtcggcggcc tggcgcgcgc tgcgccagac actgcagcgc tggcgccggc
      901 gccgcgatcg ctgaggcaca ctcggcgcat gccgcctcgc ctcttcatcg accggccgct
      961 gacggccggc gcgaccttcg cattgccggc cggcccggcg cgccacgcgc aggtgctgcg
     1021 gctgcagccg ggcgccgcgc tgaccctgtt cgacggcacc ggcggcgagt cggccgccac
     1081 cgtcgtgcag atgggccgca gcgaggtcac ggtgcggctc ggcgagcgca ccgaggtcga
     1141 tcgcgaactg ccgttccagg tcacgctcgc gctcggcatg ccggccaacg aacgcatgga
     1201 cacgctggtc gagaaggcca ccgagctcgg tgcgagcacg atccagccgc tgctgtgcga
     1261 gcgctcggtg ctgcgcctga ccggcgaacg cgccgacaag aaggtcgcgc actggcaggc
     1321 ggttgcgctc gcggcctgcg aacagagcgg gcgcacgcgc gtgccggtga tcgcgccggt
     1381 gcgcgcgttg gcgcagtggc tgcgcgagct gccgctggac gcgcggtcgc gctggatgct
     1441 cggctttcgc gacgcgcgac cggtcgcggc gttgaccatg gcgccggccg gcgtggtctg
     1501 cctcagcggg ccggagggcg gcctgtcatc ggacgaggag gcggccgcgc gcgcggccgg
     1561 tttcgaggcg gtctcgctcg gcccgcgcgt gctgcgcgcc gagaccgcgc cgctggcgct
     1621 gctggcgttc ctggcgctgt gcgccgggtg agtccgtaga ccagcgcggt cgcggcgcgg
     1681 gtgcagaatc gctcgtttcc aactggggag tgacgacatg ggcctgcttg actcggtact
     1741 cggcgcgctc gcgaacagcc agcccggtgc gcagcccccg gagcccggcg cgacgcaggg
     1801 cgtgcccggc tccgtgctgc tcagcaccgt gatcgcgatg ctggcgaacg gccagcaggc
     1861 gcagggtgcc ggcgtcggcg ggctcggcga cctgatcggc cgtttcacgc agggcggcct
     1921 gggcgacgtg atcggttcgt ggatcggtca tggccagaac ctgccggtct ccggcgacca
     1981 gctcagccag gtgctcggcc cggatgtcat cggcaagatc gcggcgcagc tcggcgtgac
     2041 gcatggtgag gcggcgacgc aactctcgca ggtgctgccc gaggccgtcg atcgcctcac
     2101 gccgcaaggg caagcgccca gcggcggtct cggcgagatc ggcgacctgc tcgggcagtt
     2161 gaccgcgcgg cgctgagcgg cgcgttcagc ccttgcgggc cgagccgggg acgaggtcga
     2221 agcggaacag gcgacattcg atcggcccgt tccacatcgg cacgcggcgc gattccttca
     2281 gccgcatcgc gctcggcagt ttcatgtcgg ggctgagcac gtaggcggtc cagccgcccg
     2341 ggtgctgcgt gtaggcgcgc ttccagtgcg acgcgagccg cgggaagaag tcgcccggcg
     2401 tgtctcggtc ttgcgcggaa gcgcgcgccg ccttgccgcc gacctcgatg cgctcgccat
     2461 aaggcgggtt gatcatcagc gtgcccggca gcccgtccgg cagctcgggc gccggtcgct
     2521 ccagcgcgtc gccgccattg aacgtgatcg cggcctcgac gccggcacgc tgtgcgttgc
     2581 ggcgcgcgaa gtcgaccatg cggaacgaca cgtcgctcgc gtggatcggc acggggctcg
     2641 gatggatgcg cgcctgcgca tggctgcgca gccgctgcaa ctcggcgcgc agcgcgtcgg
     2701 tgacgaacgg cagcaagcgc tcgaacgcga agcgccgctt cagccccggc gcgataccgc
     2761 aagcgatctg cgcggcctcg atcgcgatcg tgcccgagcc gcagcacggg tcgtgcagcg
     2821 cgccgcccgc gtcggccgtg ccgcgccagc cggcggcggc gagcatcgcc gcggcgagcg
     2881 tttccttcag cggcgcgtcg cccttgtccg cttcgtgatt tctccagccg cgcttgaaca
     2941 gcgactcgcc ggagctgtcg acatacacgg tcgcctcgcg ctcgccgagg tgcagcacga
     3001 gctgcagatc ggggtggcgc gtgtcgacgc tggggcgctc gccgctggcg tcgcgcagca
     3061 catcgcagac cgcgtccttc acgcgcagcg cggcgaagtt caggctgcgc agcgggctgc
     3121 gctgcgccgt ggtgtcgacg cgcaaggtgt gctgcggcgt gatccactgg gtccagtcga
     3181 cgctgcgcgc gagctcgtag acatcgccct cgtcgcggta cgaaccgtgc gcgacctcga
     3241 tcagcacgcg ctgcgccagc cggctctcga ggttcaggat catcacttcg agcgggtcgc
     3301 cttcgagcgc gacgccgccg cgcgcctcgg ccacgcgcgc cttcggcagc acgcgctgca
     3361 cctcttcggc gagcagcgcc tcgacccccg cggcgcacgg cagaaacagc ggcagcgcca
     3421 tcgcgctaca tcgctttgcg cagctgcgtc ggcgcgatct tcagcgcgtc gcggtacttc
     3481 gcgacggtgc gccgcgcgac ctggatgccc tgctcgtcga gcatctgcga gatctggctg
     3541 tccgagagcg gcttggcagg attctcggcc gcgacgatct gcttgatcag cgcgcgcacc
     3601 gcggtgctcg acgcgttgcc gccggcctcg gtgttcagcg acgagccgaa gaagtacttc
     3661 agctcgaagg tgccgaacgg cgtgttcatg tacttcgcgg tggtgacgcg cgagatcgtc
     3721 gactcgtgca ggccgagctc gtcggcgatc tcgcgcagca ccagcggccg catggcgagc
     3781 tcgccgtgga cgaagtagtt cttctgccgc tccacgatgg cgttggagac gcgcaggatg
     3841 gtgtcgaagc gctgctggat gttcttgatg aaccagcgcg cctcctgcag ccgctgcgac
     3901 agcgcctggc tgccctcgcc cttgtgggcc ttcagcgcgc cggcgtagat gtcgtgcacg
     3961 cgcagccgcg gcatgacctc ggggttgagc atgacgcgga agcgggcgct ggtgccgtgg
     4021 ccgatgcgcg tgacgatcac gtcgggaatg accacgttgc gctcgacgtc gacgaagcgg
//
LOCUS       DATMJB010000218        11808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_755959, whole genome shotgun sequence.
ACCESSION   DATMJB010000218 DATMJB010000000
VERSION     DATMJB010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755959"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VNU48_04815"
     CDS             <1..1200
                     /locus_tag="VNU48_04815"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI10630.1"
                     /translation="TAGHGHDFGKACIVVAGPTLVTLIPMAAAPALPAMAAKFAASFG
                     GNGALFAQLVMTIPALMLILSAPLAGVLIERLGRRPVLLASLLLFTLAGAGALVTPDA
                     VSLMTCRVLLGIAGGGMLTVCLSMAADFAEGGPRERLLGFAVAGASALAAVALVGGGR
                     LVDELGWRAPFALYLLGIPALLVAWVAVTRETSADKDRGPAVLTSLRPLSGLYLTAVM
                     LSIGMFMPSIQGPFLLVTEGVTSAATQGAVAAACAVVAALASASFGWIVRVIRPSGIF
                     VLIGSFFGVGSLVMALSHGVLTIGAGSALMGVAAGLVEATTATVILGRVSEHSRAPAI
                     GLLLSAIFLGQFLNPWIIDPLRQAFGIHGAFIAVGVAFLALAAVLAVGHARESSAAAV
                     TARRARV"
     gene            1272..2435
                     /locus_tag="VNU48_04820"
     CDS             1272..2435
                     /locus_tag="VNU48_04820"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI10631.1"
                     /translation="MLVMVVLMCSNPFMGLMFTAVGPVLPSVMNQFGAAGDGALNAQL
                     IMTIPGVGLILAGPPIGWLTQRYGAALLVRWALLVFALAGAAGMVIDNLAVFLAARFV
                     LGVAASAIATSTLALVGERFEDVRRARILSYQAAAGSAFGFVSLLLSGAMAEAWGWRA
                     PFALYLIAAPVLICALAGLPVRGPMDAAAAAEKSTAERLSSTRGSLKPLWPVFALIVV
                     VFIAVFMNAVQASFLLAADGVTSPSVQSWIMASSAVASSVGAVSYGFIRPRLGARWTF
                     ALCLIAMGVGFVTLGLSGGIAIKVLGTVIASLGAGCVGPYVGNLLLDRAAPNVRERAA
                     GLLYSATFVGDFLNPIVVTPLRYAFGIHGAFIVVGGLCAVGALIVLLRRVRSA"
     gene            complement(2446..2901)
                     /locus_tag="VNU48_04825"
     CDS             complement(2446..2901)
                     /locus_tag="VNU48_04825"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI10632.1"
                     /translation="MGGATTPSLRHSHFGVAVSDIERSTRFYCGALGFEPAETYQIAD
                     GLDKIMELDGVRLRLQFLRRADATLELICYDSPAATGPRERRKLNQYGLTHMSFWVDD
                     LAAAMDLVRMHGGTVHDNTRAVVYGNDMVYCTDPDGVRVELMQPPALLV"
     gene            complement(2905..3333)
                     /locus_tag="VNU48_04830"
     CDS             complement(2905..3333)
                     /locus_tag="VNU48_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWI10633.1"
                     /translation="MSKESTLAQIGAVEATVPRDRIVHAIQAFLASYPARGEADIQAR
                     AELFADDAVFEDPVGAKPLVGKQALVDFFRAAFAAGWVIRMKSDRIVVCGNEAVSLTQ
                     GEWGLPGTEPAKVFIVHTFAFNAEGKATRLRVFFDQNTIE"
     gene            complement(3341..4012)
                     /locus_tag="VNU48_04835"
     CDS             complement(3341..4012)
                     /locus_tag="VNU48_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI10634.1"
                     /translation="MSTVVVVGAGPLLGKSIAKGFGKRGFNVALIARRAEALQQLVSE
                     LQGEGIEAAGFAADITSDHQITEAFDKIKTTFGAVDVLEFSPTDWGKGASKLTSALAT
                     TPDSAQADFALLVQGAIRCTREVLPGMLERKRGALFYTTGYSAIKPLAFITSLGIANS
                     GLRNYAYCLSEEVSPQGVYVGTVSINAHIVPGTEGDPDRIASVYLDMYDRQDRVESIF
                     GPLTH"
     gene            complement(4023..4805)
                     /locus_tag="VNU48_04840"
     CDS             complement(4023..4805)
                     /locus_tag="VNU48_04840"
                     /inference="COORDINATES: protein motif:HMM:NF005909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI10635.1"
                     /translation="MILQDKVIMITGVGPGMGSKLALESARAGASVALLARSTGLVDE
                     LVSAIGASGGKAIGIQCDVTKVHDCERAVAATLEAFGRIDGLVNSAYGMTKMLSVEDA
                     DLDQWRSCFEITLFGALNMVRAVLPAMKSAGGGAIVNVGTMETRRPLINNGAYNVPKS
                     ALQGATRQLAAELGQHKIRVNSAVIGWMWGKPVEDHMHAYAQQSGIPFEKLIEERTSH
                     IPIGHIPPDHECAKSVLMLLSDYTSQVTGAAWDINGGEFFSM"
     gene            complement(4918..5073)
                     /locus_tag="VNU48_04845"
     CDS             complement(4918..5073)
                     /locus_tag="VNU48_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10636.1"
                     /translation="MGVTTESALRILLRRRNLHGSLRDATRQLIHQSIARIRASVGDP
                     VGRFGHG"
     gene            5225..6427
                     /locus_tag="VNU48_04850"
     CDS             5225..6427
                     /locus_tag="VNU48_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI10637.1"
                     /translation="MSAQPTPTSRIPTEHELYERARAMVPVLLSRWGEADAGSKVPDQ
                     TVAEMQQAGFFRVLQPRRYGGYEMNPRVFYNVQMILAEGCMSTAWIYGVLAVHPWQMA
                     LFDVKAQDEVWGSDTSTLIASTYMPVGKVERVDGGFKLSGRWSFSSGCEHCDWIFLGG
                     MVPPAAGETEPHYRTFMLPRSDYEIVRNWDTIGLRATGSHDIVAENVFVPEYRTHRTR
                     DDSPAARPGQAVNKSPLYTLPFAQVFVRAVSSSCLGGLQGAINIFRDTAAKQISRNFL
                     GATAKDPTAQMVISEAASAVDLMKLAIDRNFDDMMAHAEKGAAAPLESRLLYRFQSAQ
                     VPETCAFHISRLLKSCGGSGIYRSHPLTRFFLDIHAGRAHVANFADPVGRNFGGVLCG
                     LDNMDPTV"
     gene            6424..8199
                     /locus_tag="VNU48_04855"
     CDS             6424..8199
                     /locus_tag="VNU48_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWI10638.1"
                     /translation="MTPARVHGAQRRGRTAPEKATADAAALSDEFEVDVVVVGSGAGA
                     MTAAARAHDHGLKVLVVEKTELFGGTSAVSGGSVWIPNNGHIARDGGSDSEGEALTYL
                     RAAAHGAVADERLLAYVRTAPHMLRYVEERTPVRYRAVPFYADYYQTLPGAKPGYRTL
                     DPEPFDARGIGADLLRMRETSPTMLIFGRIGMTTFEAVTLLARLPGWKKLALRIGSRY
                     WLDVLWRLRSKRDRRLTMGSALVGGLRRALLDRRIDLWLNSPMQAVLSEGGRATGVTV
                     LRGGHLQAIRARRGVVLAAGGFESNQQMREQYLPQPTRAEWTAAPPCNTGDAIRAGQA
                     LGASVDLMEHAWWTPTVHVPGEEKQRGLFSERALPGCVVVNRRGERFANEAMDYLDFV
                     AAMYEDHQKTGANLPAFMVFDAAFRHKYNAGPLVSGSVIPDWRLPADWLGKAFYRGDT
                     LDELARQIGVDPRGLEDSVRRINDYAKTGVDAQFAKGGNDYDRVYGDPSVKPNPCLGP
                     IARPPFYALKLDAGEIGTKGGLLTDEHARVLDTEGRPIEGLYAIGNTSASITGPSYPG
                     AGATLGPAMTFGYIAANHIAGVPDA"
     gene            8192..9046
                     /locus_tag="VNU48_04860"
     CDS             8192..9046
                     /locus_tag="VNU48_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI10639.1"
                     /translation="MLEGKVCVVTGASRGVGRGVALALGAAGATVYVTGRSQKEGDAP
                     LPGTVLKTAADVTAAGGRGVAVVCDHADDAQVRALFERVKREHGKLDILVNCAIAIPA
                     GLTDIGPFWQKPLAMTELFGVGMRSVYVACYYAAPLLIDAKGLVVNISSAGGRCYMHG
                     PAYGGAKAATDKMSHDMAHDFKPSGVCVLALWPGIVKTERTLSVCAAEPDKYGAWLEM
                     GETPEFVGRVVHALYADPLRLERSGRVFYAAELALEYGVKDTNGAQPPSHRQFLGDPT
                     EFSAAVVE"
     gene            9070..10119
                     /locus_tag="VNU48_04865"
     CDS             9070..10119
                     /locus_tag="VNU48_04865"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HWI10640.1"
                     /translation="MLMALFGSGCATGPTYRIDDDGTVHLPARSVKLAPELSEASRRL
                     YVDTMRSAIARQGQPSPASLAELHRVALPYSAEVVRQANERVLRDHPVTVNRRSIAGV
                     PVLEVVPANIAPANKDRVLVNLHGGAFIFNAGSLAEAVPVAEAGRIRVLVVDYRMPPE
                     HPFPAALEDFLSVYRELLRSHASEAIGVYGSSAGAALTASAALYAQQKGLPMPGALGL
                     MSFGSGDSEQTLDGLDPYLSSWGKPSLMAVPTMLKFYAPNDAIDNPLVNPIHADFTRG
                     FPPSLLISGTRDLFLSGTVRLHRKMRDAGVQAELELWDGMWHGFNADSNAPLETPEAA
                     QASRVIADFFRRRLR"
     gene            10182..10940
                     /locus_tag="VNU48_04870"
     CDS             10182..10940
                     /locus_tag="VNU48_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018009066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI10641.1"
                     /translation="MPRVQDKVVFITGGASGVGRACATLLAGEGARVVIADLDAQGGQ
                     AVAGEVGLERALFVRMDISSEEEWASAMRSALERFGRIDALVNCAAILPMESIEDATL
                     AQWQRTLRVNADGAFLGCQQAIATMKASGGGSIVNLASTAALAGFQGMCAYAASKGAV
                     TALTRNVAAHCRAKGYRIRCNSIHPAGIRTPMTETIFAGADPSLLDFDSNPASGVCEP
                     VDVANMVLYLVSDESRFVNGAELRLDNALLIAIG"
     gene            10951..>11808
                     /locus_tag="VNU48_04875"
     CDS             10951..>11808
                     /locus_tag="VNU48_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWI10642.1"
                     /translation="MTAYHRLRLRTVVDETFDAKSLVFDIPEALRSAFQYRPGQFLTL
                     RIPHGERHLPRCYSLASSPLTGEPHRVTVKRVTAGRGSNWLCDGAKAGDEIEVMPPSG
                     VFSPKTLDGDFLLFAGGSGITPVFSILRSVLVAGTGRVCLIYANRDERSVIFRDELKA
                     LAATHPTRLQVIHWLDSVQGVPHVAQLAELARSMREAQCFICGPGPFMDAAVEALHGL
                     EFPASRVHVERFVSLPDEEDAIAAPSTADAEATATVRLAVQLDGETHDISCSPQESLL
                     EAMLRAGIKA"
BASE COUNT         1762 a         3895 c         4003 g         2148 t
ORIGIN      
        1 acggcgggcc acggacacga ttttggcaag gcctgcatcg ttgttgccgg cccgaccctg
       61 gtcacgctga ttcccatggc ggcagcgccc gcgttgccgg ccatggcggc gaagttcgcg
      121 gcgagcttcg gcggcaacgg agccctgttc gcacaactcg tgatgacgat tccggccttg
      181 atgctcattc tgtccgcacc gctcgccggc gtcctgatcg aaagactggg ccgacgcccg
      241 gtgctgctgg cctcgttgct gctgttcacg ttggcgggtg cgggtgcgct ggtgaccccc
      301 gatgcggtgt cgctgatgac gtgtcgcgtc ctgctcggca ttgcgggcgg cggcatgttg
      361 acggtgtgcc tgtcgatggc ggccgacttt gccgaaggtg gcccgcgcga gcggctgctc
      421 ggctttgcgg tggcgggggc ttccgccctc gctgcggtgg ccctggtggg cggcggtcgc
      481 ctggtcgatg aactgggctg gcgcgcgccg ttcgcccttt acctcctggg catacccgcc
      541 ctgctcgtgg cctgggtcgc ggtgacgcgc gagacctcgg ccgacaagga tcgaggtccg
      601 gcggtactta cttcactgcg cccgctgtcg ggcttgtacc tgacagccgt catgctgagc
      661 atcggcatgt tcatgcccag cattcagggg cccttcctgc tcgtcaccga gggcgtcacg
      721 agtgccgcca cgcagggcgc ggtggctgca gcctgtgcgg tggtggcagc actggcctcg
      781 gcgagtttcg gctggatcgt tcgcgtcatt cgtccgagcg gcatcttcgt gctgatcggg
      841 tccttcttcg gcgtcggcag cctggtcatg gccctctcgc atggcgtgct gacgattggc
      901 gcaggcagcg cgctgatggg tgtggcggcg ggtctggtcg aagcaacaac cgcgacggtg
      961 attctcggcc gggtgtcgga gcacagccgg gcgcctgcga tcgggctgct gctgagcgcg
     1021 atcttcctcg gtcagttcct gaacccctgg atcatcgacc cgttgcgcca ggccttcggc
     1081 atccacggcg cgttcatcgc cgtcggggtc gccttccttg cgctggcggc agtgctggcg
     1141 gtaggccatg cccgtgagtc gtccgccgcg gcggtcaccg cgcggcgggc acgcgtctga
     1201 tccgaatgtc ttcttcgcct cgcgaactcg acctcggcgt ccgcgcgcca tcttcagccg
     1261 gctcggcgag catgctcgtc atggtcgtcc tgatgtgcag caatcccttc atgggcttga
     1321 tgttcactgc ggtcggcccg gtgctgccga gcgtcatgaa ccagttcggc gcggccggtg
     1381 acggcgcgct caacgcccaa ctcatcatga cgatccccgg cgtcgggctc attctggccg
     1441 gcccgccgat cggctggctg acccagcgct atggtgcggc cctgctggtg cgatgggcac
     1501 tgctcgtgtt tgcgctggcg ggcgcggcgg gcatggtgat cgacaacctc gcggtcttcc
     1561 tggcggcgcg cttcgtgctc ggcgtggcgg cgtcggccat cgcgacgtcg acgctggcat
     1621 tggtgggtga gcgcttcgag gatgtacggc gcgcacggat cctttcgtat caggcggccg
     1681 ccggttctgc tttcggcttc gtctcgctgt tgctgtccgg cgcgatggcg gaggcctggg
     1741 gttggcgggc gccattcgcg ctgtacctca ttgccgcgcc ggtgctgatc tgcgccttgg
     1801 ccggattgcc cgtgcgcggt ccgatggatg ccgcggcggc cgccgaaaaa tcgaccgccg
     1861 aaaggctgtc atcgaccagg ggctctctga agccgttgtg gcccgtgttt gcgttgatcg
     1921 tcgtcgtctt catcgcggtt ttcatgaacg cagtgcaggc gtccttcctg ttggccgccg
     1981 atggtgtgac cagcccgtcg gtgcagtcct ggatcatggc gtccagcgcg gtcgccagtt
     2041 cggtcggcgc tgtgtcctat ggctttatcc gtccgcgcct gggggcgcga tggacatttg
     2101 cgctgtgcct gatcgcgatg ggtgtcgggt tcgtcacgct cggtctgtcg ggcggtatcg
     2161 cgatcaaggt actgggaacg gtcattgctt cgctcggggc cggctgcgtc ggaccgtacg
     2221 tcggcaacct gctgctcgac cgggcggccc cgaatgtgcg ggagcgtgcc gcaggtctgc
     2281 tttactccgc gaccttcgtc ggtgactttc tcaatccaat cgtggtgacg ccgctgcgtt
     2341 acgcgttcgg cattcacggc gccttcatcg tggtcggtgg actgtgcgct gtcggtgcac
     2401 tgatcgttct gttgcggcgc gtgcggtcgg cctagacggg gctggctaga cgagcagggc
     2461 ggggggctgc atcaactcga cgcgaacgcc gtcagggtcg gtgcaataga ccatgtcatt
     2521 gccgtacacg acggcgcgcg tgttgtcgtg gactgtcccg ccatgcatgc ggacgaggtc
     2581 catggccgcg gccaggtcgt cgacccagaa tgacatgtgg gtcaggccgt actggttgag
     2641 cttgcgacgc tcgcgcgggc cggtcgctgc gggcgagtcg tagcagatca gctcgagcgt
     2701 cgcgtcggca cgccggagaa actgcagtcg cagccgcacg ccgtcgagtt ccatgatctt
     2761 gtccagaccg tcggcgatct ggtacgtctc ggctggctcg aacccgaggg cgccacagta
     2821 gaagcgcgtg gaccgttcga tatcggagac ggccacgccg aagtgggaat gccgcagcga
     2881 tggtgtggtg gcgccgccca tggcctactc gatggtgttc tggtcgaaga acacccgcag
     2941 gcgcgtggct ttgccttccg cgttgaaggc aaaggtgtgc acgatgaaga ccttggcagg
     3001 ctccgtgccg ggcagccccc attcgccctg cgtcaacgaa accgcctcgt tgccgcacac
     3061 cacgatccga tccgacttca tgcgaatgac ccagccggcg gcgaatgcgg ccctgaagaa
     3121 gtcgaccagc gcttgcttgc ccaccaacgg ctttgcaccg acagggtcct cgaacacggc
     3181 gtcatcggcg aacagctcgg cgcgggcctg gatgtcggct tccccgcgtg ccgggtacga
     3241 cgccagaaat gcctggatgg cgtggacgat tcgatcacgc gggacggtcg cttcaacggc
     3301 gccgatttgc gcaagggtgg attctttcga catggatctc tcagtgggtg agggggccga
     3361 agatggactc gacccgatct tgccggtcgt acatgtccag atagacggag gcgatcctgt
     3421 cggggtcgcc ttcggtgccc ggcacgatgt gcgcgttgat cgacacggtg ccgacgtaca
     3481 ccccctgcgg cgaaacctcc tcgctcaggc agtaggcgta gttgcgcagg cccgaattgg
     3541 cgatgccgag cgacgtgatg aacgcgagcg gcttgatcgc cgagtagccg gtcgtgtaga
     3601 acagcgcgcc gcgcttgcgc tcgagcatgc cgggcagcac ttccctggtg caccggatcg
     3661 ctccctggac cagcaaggcg aagtctgcct gggcgctgtc cggggtggtg gctagcgccg
     3721 acgtcagctt gctggcgccc ttgccccagt cggtggggct gaattccagc acgtcaaccg
     3781 cgccgaaagt cgttttgatc ttgtcgaagg cctcggtgat ctgatggtcg gacgtgatgt
     3841 ccgccgcgaa tccggccgcc tcgatgcctt cgccctgcaa ctcggacacc agctgctgga
     3901 gtgcctcggc ccggcgcgcg atcaatgcga cgttgaagcc ccgtttgccg aagcctttgg
     3961 cgatcgactt gcccagcaag ggccctgcac ccaccacgac cactgtgctc atcccgtcac
     4021 ccctacatcg agaagaattc gccgccattg atgtcccatg cggctccggt gacctggctg
     4081 gtgtagtcgg acagcagcat cagcacggac ttggcgcact cgtgatcggg cgggatgtgg
     4141 ccgatgggaa tgtgcgaggt tctttcctcg atcagctttt cgaacggaat gccgctctgc
     4201 tgcgcgtagg cgtgcatgtg gtcttcgacc ggcttgcccc acatccagcc gatgaccgcg
     4261 ctgttgactc gaatcttgtg ttgcccaagc tccgccgcaa gctggcgagt cgcgccctgc
     4321 agcgccgact tcggaacgtt gtaggcgccg ttgttgatca gcggcctgcg ggtctccatc
     4381 gtgccgacgt tgacgatggc gccgccgccg gcacttttca tcgccggcag cacggctcgc
     4441 accatgttca gcgcgccgaa cagcgtgatc tcgaagcacg atcgccactg gtcgaggtcg
     4501 gcgtcttcca ccgacagcat cttggtcatg ccgtaggcgc tgttgacgag gccgtcgatc
     4561 cggccgaagg cctcgagcgt cgcggcgacg gcacgctcgc agtcgtgcac tttcgtcaca
     4621 tcgcactgga tgccgatcgc ctttccgccc gaggctccga tcgcggaaac cagttcgtcc
     4681 acgagccctg tcgagcgcgc cagcagcgcg acgctggcac ctgcgcgagc cgattcgagg
     4741 gccagcttgc tgcccatgcc cgggccaacg ccggtgatca tgatcacctt gtcttgaaga
     4801 atcatggtcg gcttcctttc gtgacccagt ttccgaccgg cttcttttca aggcatcgtc
     4861 cagtaggact aagtcgaatc gcactgcttg ccggctcgac ctcgttgccc gccgacatca
     4921 tccgtgtccg aatcggccga ccgggtcccc gaccgatgcc cgaatccgcg cgatcgactg
     4981 gtggatcagc tggcgggtgg cgtcgcgcag cgacccgtgc aggttgcgcc ggcgaagaag
     5041 gatgcgcagc gcggattcgg tggtgacgcc catggcttgc tccgtgtggg ttcaagacaa
     5101 gctcattgaa gcgcccggaa ttcgttcgaa catcgtctgt caccgttagt ctctttggac
     5161 gatgactctt gcaagggcca aaagcacgat ccgtcggact caacgccagc cgcaggaatc
     5221 atccatgtcc gcccagccga ccccgacatc ccgcattccc accgagcacg aactctacga
     5281 gcgcgcacgc gcgatggtgc cggtactgct gtcgcgctgg ggcgaggccg acgccggctc
     5341 caaggtgccc gaccagaccg ttgccgagat gcagcaggcg ggcttcttcc gcgtgctcca
     5401 gccgcgccgc tacggcggct acgagatgaa tccgcgggtg ttctacaacg tgcagatgat
     5461 ccttgccgag ggctgcatgt ccaccgcctg gatctatggc gtgctcgcgg tgcacccatg
     5521 gcagatggcg ctcttcgacg tgaaggcgca ggacgaggtg tggggcagcg acaccagcac
     5581 cctgatcgcg tcgacctaca tgccggtcgg caaggtcgag cgtgtcgacg gcggattcaa
     5641 gctgagcggt cgctggagtt tctcgagcgg ctgcgagcac tgcgactgga tcttcctcgg
     5701 cggcatggtg ccgcccgcgg ccggtgaaac cgaaccgcac taccgtacgt tcatgctgcc
     5761 ccggtccgac tacgagatcg tgcgcaactg ggacacgatc ggtctgcgcg ccaccggcag
     5821 ccacgacatc gtcgccgaaa acgtcttcgt gcccgagtac cgcacgcatc gcacccgcga
     5881 cgattctcct gcggcccggc cgggccaggc ggtcaacaag tcgccgctgt acacgctgcc
     5941 gttcgcccag gtgttcgtgc gtgccgtgtc gtcctcgtgc ctcgggggtc tgcagggggc
     6001 catcaacatc ttccgcgaca cggcagccaa gcagatcagt cgcaacttcc ttggcgccac
     6061 ggcgaaggat ccgacggccc agatggtgat ctcggaagcc gcttccgcgg tcgacctgat
     6121 gaagcttgcg atcgaccgca atttcgacga catgatggcg cacgccgaga agggcgccgc
     6181 ggcgccgctg gagagccggc tgctctatcg tttccagtcc gcacaggtgc ccgagacctg
     6241 cgcgttccac atcagccgcc tgctgaagtc gtgcggcggc tccggcatct accgcagcca
     6301 cccgctgacg cggttcttcc tcgacatcca tgccggtcgg gcccacgtgg cgaatttcgc
     6361 cgacccggtg ggccgcaact tcggtggtgt gctgtgcggt ctcgacaaca tggaccccac
     6421 ggtttgacac cggctcgcgt ccacggcgcc cagcggcggg gccgcaccgc accagagaag
     6481 gccacggcgg acgccgcggc actgagtgac gagttcgagg tcgacgtcgt ggtggtcggg
     6541 tcgggcgccg gcgcgatgac ggctgccgcg cgcgcccacg atcatggctt gaaggtgctg
     6601 gtcgtcgaaa agaccgaact gttcggcggc acctcggcgg tgtcgggcgg cagcgtctgg
     6661 ataccgaaca acgggcacat tgcgcgtgac gggggcagcg acagcgaggg cgaggcactg
     6721 acctacctgc gtgcggccgc gcacggcgcc gtcgccgacg agcgcttgct ggcctatgtc
     6781 cgcacggcgc cccacatgtt gcgctacgtc gaggaacgca cgcccgtgcg ctaccgggcg
     6841 gtgccgttct acgccgacta ctaccagacc ctgcccggtg caaagccggg ctaccgcacg
     6901 ctcgacccgg aacccttcga cgcccgcggc atcggtgccg acctgctgag gatgcgggag
     6961 acctcgccga cgatgttgat cttcggtcgc atcggcatga cgaccttcga ggccgtgacc
     7021 ctgctggcgc gcctgcccgg atggaagaag ctcgcccttc ggatcggcag ccgctattgg
     7081 cttgacgtgc tgtggcgcct gcgcagcaag cgcgatcggc gcctgaccat gggcagtgcg
     7141 ctcgtcggcg gcctgcggcg tgcgctgctg gatcggcgca tcgacctgtg gctgaactca
     7201 ccgatgcagg cggtcctcag cgaaggaggc cgcgcgacgg gtgtgaccgt ccttcgcggt
     7261 ggccaccttc aggccattcg cgcccgccgc ggcgtggtgc tggcggccgg tggcttcgaa
     7321 agcaaccagc agatgcgcga gcagtacctg ccgcagccga cccgggccga gtggaccgcg
     7381 gcgccgccct gcaataccgg cgatgcgatt cgcgcgggcc aggcactcgg cgcatcggtg
     7441 gatctgatgg agcacgcgtg gtggacgccg acggttcatg tgccgggcga agagaaacag
     7501 cgcggcctgt tctcggaacg tgcgctgccg ggctgtgtgg tggtcaaccg gcgcggtgag
     7561 cgcttcgcga acgaagcgat ggactacctc gacttcgtgg ctgcgatgta cgaggaccat
     7621 cagaagacgg gtgcgaacct gccggcattc atggtcttcg acgccgcatt ccgccacaag
     7681 tacaacgccg gaccgctggt gtccgggtcc gtcatcccgg attggcggct gccggccgac
     7741 tggctcggca aggccttcta tcgcggcgac acgctcgacg aactcgcgcg ccagatcggc
     7801 gtcgacccgc gcgggctcga ggacagcgtg cgtcgcatca acgactatgc aaagaccggc
     7861 gtcgacgccc agttcgccaa gggtggcaac gactatgacc gcgtctacgg cgatccatcg
     7921 gtcaagccga atccgtgcct cggtccgatt gccagaccac cgttctatgc gctgaagctc
     7981 gatgccggcg agatcggcac gaagggcggc ctgctgaccg acgaacatgc gcgcgtcctg
     8041 gacaccgaag gccggcccat cgaaggcctg tacgccatcg ggaacacgtc ggcctccatc
     8101 accgggccct cgtatcccgg cgccggcgcc acgctggggc cggccatgac cttcggctac
     8161 atcgccgcca accacattgc aggggttccc gatgcttgaa ggaaaagtct gtgtcgtcac
     8221 cggcgccagc cggggcgtcg gccgcggcgt ggcgctggcg ctcggcgccg cgggcgcgac
     8281 ggtctacgtg accggtcgca gccagaagga gggcgatgcg ccgctgcccg gcacggtgct
     8341 gaagactgcc gccgacgtga ccgcagccgg cgggcgcggt gtcgcagtcg tctgcgacca
     8401 cgccgacgac gcgcaagtgc gcgcgctgtt cgagcgcgtg aagcgcgagc acggcaagct
     8461 cgacatcctg gtcaattgcg cgattgccat tcccgcgggc ctgaccgaca tcgggccgtt
     8521 ctggcagaag ccgctggcga tgaccgaact gttcggtgtc ggcatgcgct cggtgtatgt
     8581 ggcctgctac tacgcggcgc cgctgctcat cgacgccaag ggtctggtgg tcaacatctc
     8641 gtcggccggt gggcgctgct acatgcacgg gccggcctat ggcggcgcga aagccgcgac
     8701 cgacaagatg tcgcacgaca tggcgcacga cttcaagccc tccggtgtct gcgtgctggc
     8761 cctgtggccg ggtatcgtca aaacggaacg aacgctgagc gtttgcgccg ccgagcccga
     8821 caagtacggc gcgtggctcg agatgggcga gacgcccgag ttcgtgggcc gggtggtgca
     8881 cgcgctgtac gcggacccgc tgcggctgga gcgctcgggc cgggtgttct atgcggccga
     8941 actggcgctc gaatacggcg tgaaggacac caatggcgca cagccgccgt cgcaccgcca
     9001 gtttctgggt gacccgaccg agttctctgc ggcggtggtc gaatgaggtg cctggccgcc
     9061 cttggcgcga tgctgatggc gctgttcggt tcgggctgcg cgaccggccc gacttaccgc
     9121 atcgacgatg acggcaccgt gcacctgcct gcgcgcagcg tcaagttggc gccggaattg
     9181 agcgaagcct cacgtcggct ctatgtcgac accatgcgct cggcgatcgc gcggcaaggc
     9241 cagccgtcgc ccgcatcgct ggccgaactc catcgcgtgg cgctcccgta ttccgccgag
     9301 gtggtgcgac aagcgaatga gcgcgttctg cgcgaccacc cggtcacggt gaaccggcgt
     9361 tcgatcgccg gcgtgcccgt gcttgaagtc gttccggcga acatcgcacc ggccaacaag
     9421 gaccgtgtgc tcgtcaacct gcacggcggg gccttcatct tcaacgccgg ttcgctggcc
     9481 gaggccgtgc ccgtcgccga ggcggggcgg attcgcgtac tggtggtgga ctatcggatg
     9541 ccgcccgagc atccctttcc ggcggcgctg gaggatttct tgagcgtcta ccgtgagctg
     9601 ctgcgcagcc atgcgagcga ggccatcggc gtctacggga gttccgcagg cgccgcactg
     9661 accgcgtcgg ccgcactcta cgcgcagcag aagggcctgc ccatgccggg tgccttgggc
     9721 ctcatgtcat tcggcagcgg cgacagtgag cagactctcg atggcctcga tccatacctg
     9781 tcctcgtggg gcaaaccgtc tctgatggcc gtgccgacga tgctgaagtt ctatgcaccc
     9841 aacgacgcca tcgacaaccc gctcgtcaac ccgatccatg cggacttcac gcgcggtttt
     9901 ccaccctcgc tgctgatcag cggcacccgg gatctgtttc tcagcggaac ggtgcgattg
     9961 caccggaaga tgcgcgacgc cggggtgcaa gccgagctgg agctgtggga cggcatgtgg
    10021 cacgggttca acgccgattc gaacgcgcca ctggaaacac ccgaggcggc gcaggccagc
    10081 cgcgtgatcg ccgacttctt ccgccggcgc ctgcgctagt ctcgttggac gatgaggcgc
    10141 ccgctggcgc actcacaatg gaactgcagg agacaagcgc gatgccaaga gtccaggaca
    10201 aggtcgtatt catcaccggc ggtgcgagcg gcgtcgggcg cgcctgcgcg accttgttgg
    10261 cgggcgaggg tgcacgcgtg gtgatcgccg atctggatgc acaaggcggc caggcggtgg
    10321 ccggcgaagt cggcctcgag cgcgcgctgt tcgttcgcat ggacatctcg agcgaggaag
    10381 agtgggccag cgcgatgcgc tcggcgctgg agcgttttgg ccgcatcgac gcgctggtca
    10441 actgcgccgc catcctgccg atggaatcga tcgaggacgc gacgctggcg cagtggcaac
    10501 gcacgctgcg ggtgaacgcc gacggcgcct tcctcggttg ccagcaggcc atcgccacca
    10561 tgaaggcgag cggcggcggc tcgatcgtga atctggcgtc caccgcggct ctggcgggct
    10621 ttcagggcat gtgcgcgtat gccgcctcca aaggcgcggt gacggcgctc acgcggaacg
    10681 tggcggccca ttgccgggcc aagggctacc gcatccgctg caactccatt caccccgcgg
    10741 gaatccgcac gccgatgacc gagacgattt tcgccggggc agacccctcc ttgctcgact
    10801 tcgacagcaa cccggcctcc ggcgtgtgcg agcccgtcga cgtcgccaac atggttctct
    10861 atctcgtgtc cgacgaatcg cgcttcgtca atggcgccga gctgcgcctg gacaacgcgc
    10921 ttctcatcgc catcggttga tgtcttgtcc gtgaccgcct atcaccgcct ccgcctgcgt
    10981 acggtcgtcg atgagacctt cgatgccaag tcgctcgtgt tcgatattcc ggaggcgctg
    11041 cgctcggcgt tccagtaccg cccgggccaa ttcctgacgc tgcgcattcc gcacggcgag
    11101 cgccatttgc cgcgctgcta ttcgctggcc agttcgccgc tgaccgggga gccacaccgc
    11161 gtcacggtca agcgcgtgac cgcggggcgc ggctcgaact ggctgtgcga cggcgccaag
    11221 gccggggacg agatcgaggt gatgccgccg tcgggtgtgt tcagccccaa gacgctggac
    11281 ggcgacttcc tgctctttgc cgggggcagc ggcatcacgc ccgtcttctc gatcctgcgc
    11341 tcggtgctgg tggccggcac gggaagggtg tgcctgatct acgccaaccg cgacgagcgt
    11401 tcggtgatct tccgcgacga gctcaaggcg ctcgccgcga cgcatccgac gcggcttcaa
    11461 gtcatccatt ggctcgattc ggtgcagggc gtgccgcatg tggcccagct cgccgaactg
    11521 gcgcgctcga tgcgcgaagc ccagtgcttc atctgcgggc cgggtccgtt catggatgcc
    11581 gcggtcgagg ccttgcacgg tctcgagttt cccgcctcac gcgttcacgt ggagcgcttc
    11641 gtgtcgctgc ccgacgagga ggatgcgatt gccgcgccct cgactgcgga tgccgaggcc
    11701 actgcgaccg tgcggctcgc cgtgcaactc gacggtgaga ctcacgatat ttcctgctcg
    11761 ccgcaggaga gcctgctcga agcgatgctg cgcgccggca tcaaggcg
//
LOCUS       DATMJB010000219         4772 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_783869, whole genome shotgun sequence.
ACCESSION   DATMJB010000219 DATMJB010000000
VERSION     DATMJB010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783869"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1363
                     /gene="lpdA"
                     /locus_tag="VNU48_04880"
     CDS             <1..1363
                     /gene="lpdA"
                     /locus_tag="VNU48_04880"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWI10643.1"
                     /translation="AAQLGFNTACIDEWKNDKAGPALGGTCTNIGCIPSKALLQSSEH
                     FEHAGHAFADHGIQLENLSIDVPKMLARKATVVKQNNDGILYLFKKNKVTFFHGRGSF
                     VKTGAEGSEIAVAGDKPETLIAKHVIIATGSNPRALPGAAFDEDNILSNDGALRIPGV
                     PKTLGVIGSGVIGLEMGSVWRRLGADVTVLEALPTFLGAVDEQIAKEAHKAFTKQGLK
                     IQLGVKISAVTSTKKGVTVAYADAAGKEQKLECDKLIVSIGRVPNTIGLNAEAIGLKL
                     DERGFIAVDDDCRTNVPNVWAIGDVVRGPMLAHKAEEEGVAVAERLKGQHPHVDFNTV
                     PWVIYTSPEIAWVGQTEAQLKAAGRAYKAGTFPFMANGRARALGDTTGLVKVLADAKT
                     DEILGVHMVGPMVSELISEAVVAMEFKASAEDIARICHAHPSLAEATKEAALAVDKRT
                     LNF"
     gene            1384..2481
                     /gene="zapE"
                     /locus_tag="VNU48_04885"
     CDS             1384..2481
                     /gene="zapE"
                     /locus_tag="VNU48_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HWI10644.1"
                     /translation="MPVRALYERTLAERGYTADPAQLRALDSLERCENEWAAYKARRG
                     NALTKLIARPPIPRGVYMYGGVGRGKSFLMDCFFNAVPLQRKTRLHFHEFMREVHREL
                     SELQGTVNPLQELGRRIARRYRLICFDEFHVSDVTDAMILHRLLEALFANRVSIVTTS
                     NFKPDNLYPNGLHRDRILPAIELLKTRLEVVNVDNGSDYRQITLAQVGLYQTPLNAAA
                     EAAMTHAFERLAEAKDESPLLHIEHREIRSRRRAGGVVWFDFKELCGGPRSQNDYLEL
                     ATQFHTLLLSGVPAMSPRMASEARRFTWLVDVLYDRRVKLIVSAEVEPEGLYTEGPLA
                     HEFPRTVSRLREMQSAEFLALARRDVDTSLT"
     gene            2478..3074
                     /locus_tag="VNU48_04890"
     CDS             2478..3074
                     /locus_tag="VNU48_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10645.1"
                     /translation="MKPRLLCLMLACAASAGTWAADAEHDRIAAERASADARLAAQQR
                     ECAARFVVAACVEDARAEHRATLARLRQQQLQIDETRRRETAAARRKAIAERVEAQQA
                     RASDVPPEAPRVNVRRAPEPAPALRPAEPMLPRAPAVGASSADRATFEQRNQAKFDAR
                     ARAAQAHREDVERRNARRAAQGKVAAPLPAASGASTAR"
     gene            complement(3085..3852)
                     /locus_tag="VNU48_04895"
     CDS             complement(3085..3852)
                     /locus_tag="VNU48_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HWI10646.1"
                     /translation="MSTATSAYRLTGTTGLHRGDRAYQQDQVEILPHDRVPGCLLAVL
                     ADGMGGKSGGRKAADQVLLTANQLFERYSPSRDDTGEALKQLVLEAHLMIKLTAITSE
                     EEPHSTVAAFIVSPSRDCDIIHAGDSRIYHFRGAELVSRTVDHSYVQRLVNEGQITED
                     EANAHPQSNLLTGCLGTNADPPLSHKHLGRLEIGDSLLACSDGLWHYFTARELGAIVH
                     ALPPREAGEMLVGKARQRARGGGDNLSLALVRLEALE"
     gene            3931..4134
                     /locus_tag="VNU48_04900"
     CDS             3931..4134
                     /locus_tag="VNU48_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HWI10647.1"
                     /translation="MDLNLHSPPRRLIELRMEHADLDNLIDQAALTLPDDELSLRRLK
                     KRRLLLRDQIARLEAELDPPEPA"
     gene            4131..>4772
                     /locus_tag="VNU48_04905"
     CDS             4131..>4772
                     /locus_tag="VNU48_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWI10648.1"
                     /translation="MSPSPLEEAVAAAFARGGALARADAHHVEREVQLRMALGVAQAI
                     DARSALVVEAGTGVGKTFAYLVPTLLSGARALVSTATKSLQDQLFLRDIPRLREALQL
                     PVTVALLKGRSSYLCLHRMNQARLAETLPDRWAVRTLAKVEQWSHVTVSGDLAELDGL
                     DERSSVIPLVTSTRENCLGQECPQFRQCHVVKARREAMLADVVVVNHHLFFADM"
BASE COUNT          773 a         1653 c         1654 g          692 t
ORIGIN      
        1 agccgcgcaa ctcggcttca ataccgcttg catcgacgag tggaagaacg acaaggcggg
       61 cccggcgttg ggcggcacct gcaccaacat cggctgcatt ccgtcgaagg cgctgctgca
      121 atcgtccgag cacttcgagc acgccggcca cgcgttcgcc gaccacggca tccagctgga
      181 gaacctctcc atcgacgtgc cgaagatgct ggcgcgcaag gccaccgtcg tgaagcagaa
      241 caacgacggc atcctgtacc tgttcaagaa gaacaaggtc accttcttcc acggccgcgg
      301 ttcgttcgtg aagaccggtg ccgagggcag cgaaatcgcg gtcgccggcg acaagcccga
      361 gacgctgatc gcgaagcacg tgatcatcgc gaccggctcg aacccgcggg ccttgcccgg
      421 tgccgccttc gacgaagaca acatcctgtc gaacgacggc gcgctgcgca tccccggcgt
      481 gcccaagacg ctgggcgtga tcgggtccgg cgtgatcggc ctcgagatgg gctcggtatg
      541 gcgccgcctc ggcgccgacg tgaccgtgct cgaagcactg ccgaccttcc tcggtgcggt
      601 cgatgaacag attgcaaagg aagcgcacaa ggccttcacg aagcaggggc tgaagatcca
      661 gctcggcgtg aagatcagcg cggtgacgtc gaccaagaag ggcgtgacgg tcgcctacgc
      721 cgacgcggcc ggcaaggagc agaagctcga gtgcgacaag ctcatcgtgt cgatcggccg
      781 ggtgccgaac acgatcgggc tgaacgccga agcgatcggg ctgaagctcg acgagcgcgg
      841 cttcatcgcg gtcgacgacg actgcaggac caatgttccc aacgtctggg ccatcggcga
      901 cgtggtgcgc ggcccgatgc tcgcgcacaa ggccgaggaa gagggtgtcg cggtcgccga
      961 gcggctcaag ggccagcacc cgcacgtcga cttcaacacc gtgccctggg tgatctacac
     1021 ctcgcccgag atcgcctggg tcgggcagac cgaagcgcag ctcaaggccg cgggccgcgc
     1081 ctacaaggcc ggcacgttcc cgttcatggc gaacggccgg gcgcgcgcgc tcggcgacac
     1141 caccgggctg gtgaaggtgc tggccgatgc gaagaccgac gagatcctgg gcgtgcacat
     1201 ggtcgggccg atggtctcgg agctgatctc cgaagccgtg gtcgcgatgg agttcaaggc
     1261 ttcggcggaa gacatcgcgc gcatctgcca cgcgcacccg tcacttgccg aagccacgaa
     1321 ggaagccgca ctggcggtcg acaagcgcac gttgaacttc tgatccggtc gacggcagca
     1381 cccgtgcccg tccgcgccct ctacgaacgc acgctggccg agcgcgggta cacggcggac
     1441 ccggcccagt tgcgtgcgct cgactcgctc gagcgctgcg agaacgaatg ggccgcctac
     1501 aaggcgcggc gcggcaatgc gctgacgaag ctgatcgcgc gcccgccgat cccgcgcggc
     1561 gtctacatgt acggcggcgt cgggcgcggc aagagcttcc tgatggattg cttcttcaac
     1621 gccgtgccgc tgcagcgcaa gacgcggctg catttccacg agttcatgcg cgaggtgcac
     1681 cgcgagctga gtgaattgca gggcacggtc aatccgctgc aggaactcgg caggcgcatc
     1741 gcacggcgct accgcctgat ctgcttcgac gagttccacg tctcggacgt gaccgacgcg
     1801 atgatcctgc accgcctgct ggaagcgctg ttcgcgaacc gcgtcagcat cgtcacgacc
     1861 tcgaacttca agcccgacaa cctgtacccg aacggcctgc accgcgaccg catcctgccg
     1921 gcgatcgagc tgttgaagac caggctcgag gtcgtcaacg tcgacaacgg cagcgactac
     1981 cggcagatca cgctggcgca ggtcggcctg taccagacgc cgctgaacgc ggccgccgag
     2041 gccgccatga cgcacgcgtt cgagcgcctc gccgaggcga aggacgagtc gccgctgctg
     2101 cacatcgagc accgcgagat ccgttcgagg cggcgcgccg gcggcgtcgt ctggttcgac
     2161 ttcaaagagc tgtgcggcgg gccgcgctcg cagaacgact acctcgagct cgcgacccag
     2221 ttccacacgc tgctgctgtc gggcgtgccg gcgatgtcgc cgcgcatggc ctcggaggcg
     2281 cgacgcttca cctggctcgt cgacgtgctg tacgaccggc gcgtcaagct gatcgtgtct
     2341 gccgaggtcg agcccgaggg cctgtacacc gaggggccgc tggcgcacga gtttccgcgc
     2401 acggtgtcac gactgcgcga gatgcagtcc gccgagttcc tggcattggc gcggcgcgat
     2461 gtggatactt cgctgacatg aagccccgac tcctttgcct gatgctggcg tgcgccgcgt
     2521 cggccggcac ctgggccgcc gacgccgagc acgaccggat cgccgccgag cgcgcctcgg
     2581 ccgacgcgcg gctcgccgcg cagcagcgcg aatgcgcggc gcgtttcgtg gtcgccgcct
     2641 gcgtcgagga cgcgcgtgcc gagcatcgcg cgacgctggc acggctgcgc cagcagcagc
     2701 tgcagatcga cgagacgcgg cgccgcgaga ccgctgcggc ccgccgcaag gcgatcgccg
     2761 agcgcgtcga ggcgcagcag gcgcgcgcca gcgacgtgcc gcccgaggcg ccgcgcgtga
     2821 acgttcggcg tgcgcccgag cctgcgcccg cgctgcggcc ggccgagccg atgctgccgc
     2881 gcgcgcctgc ggtcggcgcc tccagcgccg accgggcgac gttcgagcag cgcaatcaag
     2941 ccaagttcga tgcccgcgcg cgcgcggcgc aagcgcatcg cgaggacgtg gagcgccgca
     3001 acgcgcggcg cgcagcgcaa ggaaaggtgg cggcgccgct gccggccgcg tcgggcgcct
     3061 cgacggcgcg ctgaacgcgg ctcgctattc gagcgcctcg agccgcacca gcgcgagcga
     3121 caggttgtcg ccaccgccgc gggcgcgctg gcgtgccttg ccgaccagca tctcgccggc
     3181 ctcgcgcggc ggcagcgcgt gcacgatcgc cccgagttcg cgcgcggtga agtagtgcca
     3241 caggccgtcg ctgcaggcga gcaggctgtc gccgatctcg agccgcccga ggtgcttgtg
     3301 cgacagcggc gggtcggcgt tggtgcccag gcagccggtc agcaggttcg actgcgggtg
     3361 cgcgttggcc tcgtcctcgg tgatctggcc ttcgttgacg aggcgctgca cataggaatg
     3421 gtcgaccgtg cggctgacca gctcggcgcc gcggaagtgg tagatgcgcg aatcgcccgc
     3481 atggatgatg tcgcagtcgc gcgaggggct gacgatgaag gcggcgaccg tgctgtgcgg
     3541 ttcttcttcc gaggtgatcg ccgtcagctt gatcatcagg tgcgcctcga gcacgagctg
     3601 cttcagcgct tcgccggtgt cgtcgcggct cggcgagtag cgctcgaaga gctggttcgc
     3661 ggtcagcagc acctggtcgg cggccttgcg accgccgctc ttgccgccca tgccatcggc
     3721 cagcaccgcc agcaggcaac ccggcacgcg gtcgtgcggc aggatctcga cctggtcctg
     3781 ctggtaggca cggtcgcctc gatgcaggcc cgttgtgccg gtcaggcggt aggccgaggt
     3841 cgctgtgctc atggtcgaaa actataatcg gtttgcattc gttcacgccg cccaataccg
     3901 cacagattcg cccccgacag cgcgcgcccc atggatctca acctgcattc cccgccgcgc
     3961 cggctgatcg agctgcgcat ggagcatgcc gatctcgaca acctgatcga tcaggcggcg
     4021 ctgaccctgc ccgacgacga actgagcctg cgccgcctga agaaacgccg cctgctgctg
     4081 cgcgaccaga tcgcgcggct cgaagcggag ctcgatccgc ccgagccggc gtgagcccga
     4141 gcccgctgga agaagcggtg gcggcggcgt ttgcgcgcgg cggcgcgctc gcgcgcgccg
     4201 atgcccacca tgtcgaacgc gaggtccaac tgcgcatggc gctgggcgtc gcgcaggcga
     4261 tcgacgcgcg cagtgcgctg gtggtcgagg ccggcaccgg cgtcggcaag accttcgcct
     4321 acctcgtgcc gacgctgctg agcggggcgc gtgcactcgt cagcaccgcg accaagagcc
     4381 tgcaggacca gctgttcctg cgcgacattc cgcgcctgcg cgaggccctg cagctgcccg
     4441 tcacggtggc gctgctgaag ggccgcagca gctacctctg cctgcaccgc atgaatcagg
     4501 cgcgcctggc cgagaccctg cccgaccgct gggccgtgcg cacgctcgcg aaggtcgagc
     4561 agtggtcgca cgtgaccgtc agcggcgatc tggccgaact cgacggcctc gacgagcgca
     4621 gcagcgtgat tccgctggtc acgtcgacgc gcgagaactg cctcggccag gagtgcccgc
     4681 aattccgcca gtgccacgtc gtcaaggcgc ggcgcgaggc gatgctggcc gacgtggtgg
     4741 tcgtcaacca ccacctgttc tttgccgaca tg
//
LOCUS       DATMJB010000220         2646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_723662, whole genome shotgun sequence.
ACCESSION   DATMJB010000220 DATMJB010000000
VERSION     DATMJB010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723662"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..>2646)
                     /locus_tag="VNU48_04910"
     CDS             complement(151..>2646)
                     /locus_tag="VNU48_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HWI10649.1"
                     /translation="YQKDEKVWLELQPGDFDKPFFLSPKIATGIGEAFVFGGLLLGGG
                     EQVIEFRRVHNQVQMIARNTEFTARAGTPEGRAVAAAFSPSLLASTPVASQPQPERKS
                     VLVDAGALFVNDMLGIGMQLQRSYRQGYGFDGRNSAITALRARPDGVALEVLQHFATG
                     SIAVRQPNTPPGAPVPTVPSTVPDPRSLFVTAHYTIAALPAMPMPARAADARIGYFNA
                     TQWDFSDDLARTPLVRHVDRWRLEKKDPAAALSEPVRPIVYWLDRTIPLKYRDTISAG
                     ILEWNKAFERIGFKDAIQVKVPDDADFDTLDGGASVRWMTNAAPSFGATGPSHVDPRS
                     GEILAANIGIESLSSRYIRASRSQILTGSPTAYAMLMQFGGAAAGEAFDAHACINAEQ
                     AADQLSYALDVLGARGDIDPDSPQAEAFVLAYLKEVTMHEVGHTLGLRHNFRASRAYS
                     DAQLSDPEFTRTHALTGSVMEYAPVNLASPGARQVAPFQTTLGPYDYWAIEYAYRPLA
                     PADEKAALERIAARSSEPELAFGSDEDNFLGIDPDALQFDLGNDPLAFARKRLAIARD
                     LFKRQETRALSPDNDYAVLRRSLNFAVNDVARSVGVLARQIGGVRTLRDFPGSGRDPL
                     QPVPAPIQRDALDQIARGLFAADSLPVSAALQRRLAPDFQERIDALERGSAAVATEYS
                     VTQRVLGVQRELLNQLMGDEVAARIIDSQGKVATRGDAFQLSELYGRLGRDIWSELDS
                     AGTARAADIPAPRRALQREHLNRLTALLLRPNGAGSADARSLVRAQAQALLLRLNAAS
                     QRAGLGADTRAHLQDSADTLAQALAARMARPGA"
BASE COUNT          339 a          901 c          998 g          408 t
ORIGIN      
        1 ggggcggctt cgtgttcagc gccggcgtgg cggagccggt gcgcacgcgg ctcgtgcgca
       61 gccgccacgg cgactcgtcg ggcgtgcgcg gcgccgcgtg gttgtggcgc gatcgctgag
      121 ttccggccac gacattcatc cgcggaaggt tcaggccccc ggccgcgcca tccgcgctgc
      181 cagcgcctgc gccagcgtgt cggcgctgtc ctgcagatgc gcgcgcgtgt cggcgccgag
      241 cccggcccgt tgtgacgcgg cgttcagccg cagcagcagc gcctgcgcct gggcccgcac
      301 caggctgcgc gcgtcggcgc tgccggcacc gttcgggcgc agcagcagcg cggtcaggcg
      361 gttcagatgc tcgcgctgca aggcgcggcg cggtgccggg atgtcggcgg cgcgcgccgt
      421 gccggcgctg tcgagttcgc tccagatgtc gcggcccagg cggccgtaga gctcggacag
      481 ctggaacgcg tcgccgcgcg tcgccacctt gccctggctg tcgatgatgc gcgcggcgac
      541 ctcgtcgccc atcagctggt tcagcagttc gcgctgcacg ccgagcacgc gctgggtcac
      601 ggagtattcg gtcgccaccg ctgcgctgcc gcgttcgagc gcgtcgatgc gctcctgaaa
      661 gtccggcgcg aggcgccgct gcagcgccgc cgacaccggc aggctgtcgg cggcgaacag
      721 gccgcgcgcg atctggtcga gcgcgtcgcg ctggatcggc gccggcaccg gctgcagcgg
      781 gtcgcggccc gagccgggga agtcgcgcag cgtgcgcacg ccgccgatct ggcgtgccag
      841 cacgcccacc gatcgcgcca cgtcgttgac cgcgaagttc agcgagcggc gcagcaccgc
      901 gtagtcgttg tcgggcgaga gcgcgcgcgt ttcctggcgc ttgaacaggt cgcgtgcgat
      961 cgccagacgc ttgcgtgcga atgccagcgg gtcgttgccg agatcgaact gcagcgcgtc
     1021 cgggtcgatg ccgaggaagt tgtcctcgtc gctgccgaag gccagctcgg gctcgctgct
     1081 gcgcgccgcg atgcgttcga gcgcggcctt ctcgtcggcc ggcgcgagcg gcctgtaggc
     1141 gtactcgatg gcccagtagt cgtagggccc gagcgtggtc tggaacggcg ccacctggcg
     1201 cgcaccggga ctcgccaggt tgaccggcgc gtactccatc accgagccgg tcagcgcgtg
     1261 ggtgcgcgtg aactcggggt cggacagctg cgcgtcgctg taggcgcgcg aggcgcggaa
     1321 gttgtggcgc aagcccagcg tgtggccgac ctcgtgcatc gtcacttcct tcaggtaggc
     1381 gagcacgaac gcttcggcct gtgggctgtc ggggtcgatg tcgccgcgcg caccgagcac
     1441 gtcgagcgcg tagctgagct ggtcggcggc ctgttcggcg ttgatgcagg catgggcgtc
     1501 gaaggcttcg ccggcggcgg caccgccgaa ctgcatcagc atcgcgtacg ccgtcgggct
     1561 gccggtcagg atctgcgaac gcgaggcccg gatatagcgc gacgacaggc tctcgatgcc
     1621 gatgttcgcg gccaggatct cgccgctgcg agggtcgacg tggctgggcc cggtggcgcc
     1681 gaagctcggc gcggcgttgg tcatccagcg caccgaggcg ccgccgtcga gcgtgtcgaa
     1741 gtcggcgtcg tcgggcacct tgacctggat cgcgtccttg aagccgatgc gctcgaaggc
     1801 cttgttccac tcgaggatgc cggcgctgat ggtgtcgcgg tacttcagcg ggatcgtgcg
     1861 gtccagccag tagacgatcg gcctgaccgg ctccgacagc gccgccgccg ggtccttctt
     1921 ctcgaggcgc cagcggtcga catggcgcac gagcggcgtg cgcgcgaggt cgtcgctgaa
     1981 gtcccattgc gtggcgttga aatagccgat gcgcgcgtcg gcggcgcgcg ccggcatcgg
     2041 catcgcgggc aacgcagcga tcgtgtagtg cgcggtcacg aacaggctgc gcgggtcggg
     2101 cacggtcgac ggcacggtcg gcaccggcgc accgggcggc gtgttcggct gacgcaccgc
     2161 gatcgagccg gtcgcgaaat gctgcagcac ttccagtgcc acgccgtcgg ggcgcgcgcg
     2221 cagcgcggtg atcgccgagt tgcggccgtc gaagccatag ccctggcggt agctgcgctg
     2281 cagctgcatg ccgatgccca gcatgtcgtt gacgaacagc gcgccggcgt cgaccagcac
     2341 cgacttgcgc tcgggctgcg gctggctcgc caccggcgtg ctggcgagca ggctcggcga
     2401 gaacgccgcg gcgaccgcac ggccttcggg cgtgccggcg cgcgcggtga actcggtgtt
     2461 gcgcgcgatc atctgcacct ggttgtgcac gcggcgaaac tcgatcacct gctccccgcc
     2521 gcccagcagc agcccgccga agacaaaggc ttcgccgatg ccggtggcga tcttgggcga
     2581 caggaagaag ggcttgtcga agtcgccggg ctgcagttcg agccagacct tctcgtcctt
     2641 ctggta
//
LOCUS       DATMJB010000221         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_151989, whole genome shotgun sequence.
ACCESSION   DATMJB010000221 DATMJB010000000
VERSION     DATMJB010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151989"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1785
                     /locus_tag="VNU48_04915"
     CDS             <1..1785
                     /locus_tag="VNU48_04915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI10650.1"
                     /translation="FTRLTGYTADEVIGRTAVELGLWHDPASRGQLLALLARDGKVTE
                     LPVEFVTRSGARVSALVSAGRFAMDQRDYMVVNARDVTESERTRLQHVAILERASIGI
                     AFTRAGRFVHANPFFERMFGWDPGALAGQHGAVVWISDADYRELGALATPLLAAGQPF
                     QLEHRMRRRDDSQFWCRLLAQAIDRDDPSRGGTIWIAEDITERRRLDEALAAARDAAE
                     AASRAKSAFLANTSHEIRTPLNGLLGLAHLAMQQELPEARRQQYLTQIVDSAQALAGI
                     MSDILDVSKIEAGKLALDDSPFDLRDALRAVHHAYETLAQVKGLTLLLAIDERLPATL
                     RGDAVRVRQILSNFITNALKFTERGHVRIEATRTDAGLRLAVSDTGPGVDAEIQARLF
                     TPFSQGDSSTTRRFGGTGLGLSICRELAQMMGGTVGVDSTPGQGSTFWAELPLPSSAP
                     AQRGASSEAGDLARLAGMRVLMAEDNPVNMMIAVAMLEHWGARVEQASDGRAAVDAVH
                     AAARDGDPFDVVVMDVQMPVMGGHDAARELRRHYDRDALPIVALTAAALVSEREEALA
                     AGMDDFLTKPIDAVKLRHTLANLLQRHA"
     gene            complement(1814..2107)
                     /locus_tag="VNU48_04920"
     CDS             complement(1814..2107)
                     /locus_tag="VNU48_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10651.1"
                     /translation="MDTSDDSTRLVQLLEEIRDGQKLQLERQREALELQREQFAVFQA
                     QAERSERIQRKAELLQDRGASLVGGARKAVAVILPVIVLLLVYLSWLLFRSRF"
BASE COUNT          338 a          804 c          801 g          301 t
ORIGIN      
        1 ttcacgcgcc tcaccggcta cacggccgac gaggtgatcg ggcgcaccgc ggtcgaactc
       61 ggcctgtggc atgaccccgc ctcgcgcggc cagctgctcg cgctgctcgc gcgcgacggc
      121 aaggtcaccg agctgccggt cgagttcgtc acgcgctcgg gcgcgcgcgt ctcggcgctg
      181 gtgtcggccg ggcgcttcgc gatggaccag cgcgactaca tggtggtgaa cgcgcgcgac
      241 gtgaccgaga gcgaacgcac ccggctgcag cacgtcgcga tcctggagcg cgcctcgatc
      301 ggcatcgcgt tcacgcgcgc cgggcgcttc gtgcacgcga acccgttctt cgaacgcatg
      361 ttcggctggg acccgggcgc gttggccggg cagcacggcg cggtggtctg gatcagcgac
      421 gccgactacc gcgagctcgg cgcgctcgcc acccccttgc tcgcggcggg ccagccgttc
      481 cagctcgagc accggatgcg caggcgcgac gacagccagt tctggtgtcg cctgctcgcg
      541 caggcgatcg accgcgacga tccgagccgt ggcggcacga tctggatcgc cgaggacatc
      601 accgagcgcc gccgcctcga cgaggcgctg gccgccgcgc gcgacgccgc cgaggccgcg
      661 agccgggcga agagcgcgtt cctggccaac accagccacg agatccgcac gccgctgaac
      721 ggcctgctcg gtctggcgca cctggcgatg cagcaggagc tgccggaagc gcggcgccag
      781 cagtacctga cgcagatcgt cgacagcgcg caggcgctgg cgggcatcat gtcggacatc
      841 ctcgacgtct cgaagatcga ggccggcaag ctcgccctcg acgactcgcc cttcgacctg
      901 cgcgacgcgc tcagggcggt gcaccacgcc tacgaaacgc tggcccaggt caagggcctg
      961 acgctgctgc tggcgatcga cgaacggctg ccggccacgc tgcgcggcga cgcggtgcgg
     1021 gtgcgccaga tcctgtcgaa cttcatcacc aacgcgctca agttcaccga gcgcggccat
     1081 gtgcgcatcg aggccacgcg caccgacgcc gggctgcggc tggcggtgtc cgacaccggg
     1141 ccgggtgtcg atgcggagat ccaggcgcgc ctgttcacgc cgttctcgca gggcgactcg
     1201 tcgacgacgc ggcgcttcgg cggcacgggt ctgggcctgt cgatctgccg cgaactcgcg
     1261 cagatgatgg gcggcacggt cggcgtcgac agcacgccgg gccagggcag caccttctgg
     1321 gccgagctgc cgctgcccag cagcgcaccg gcccagcgcg gcgcgagcag cgaggccggc
     1381 gatctggcgc ggctggccgg catgcgcgtg ctgatggccg aggacaaccc ggtcaacatg
     1441 atgatcgcgg tggcgatgct cgagcattgg ggcgcgcgcg tcgagcaggc gagcgacggc
     1501 cgcgccgcgg tcgacgcggt gcatgccgcg gcgcgcgacg gcgacccctt cgacgttgtc
     1561 gtgatggacg tgcagatgcc ggtcatgggc ggccacgatg ccgcgcgtga actgcgccgt
     1621 cactacgatc gcgacgcgct gccgatcgtc gcgctgaccg cggccgcgct ggtgtcggag
     1681 cgcgaggagg cgctggccgc cggcatggac gacttcctga ccaagcctat cgacgcggtc
     1741 aagctgcgcc acacgctggc caacttgctg cagcgccacg cctgagcgcg cacggccgct
     1801 tacgccgatg ccgtcagaaa cgcgaacgaa acaacagcca ggacaggtac acgagcagca
     1861 gcacgatcac cggcaggatc acggccaccg ccttgcgcgc gccgccgacg aggctggcgc
     1921 cgcgatcctg gagcagctcg gccttgcgct ggatccgttc gctgcgctcg gcctgcgcct
     1981 ggaacacggc gaactgctcg cgctgcagtt ccagcgcctc gcgctggcgc tcgagctgca
     2041 gcttctgacc gtcccggatc tcctcgagca actgcacaag ccgggtcgag tcgtcgctgg
     2101 tgtccatggt ctgcgcagat gaggtggttc gggggcgccg tcaatacctg acgaagcagg
     2161 gaatcgcgat gagcgccacg ccggcgccga acacgacgcc ccacagcagc gatgcggtgt
     2221 tcacgcgtgc gcgccccggg tcag
//
LOCUS       DATMJB010000222         4752 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_131480, whole genome shotgun sequence.
ACCESSION   DATMJB010000222 DATMJB010000000
VERSION     DATMJB010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131480"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..471
                     /locus_tag="VNU48_04925"
     CDS             31..471
                     /locus_tag="VNU48_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HWI10652.1"
                     /translation="MPDPQLIAAHLLRLDGAQPALLGSRCCACGEVYFPAAHGCTRCC
                     GTRLEAFDLGHRGRLWSWTVQDFCPKPPYDGAENEADFRSYGVGYVEMACGIKVESRL
                     TLADPARLHIGMAMELTLDPYRHHTDGTAIHTYAFRPLRDEAST"
     gene            468..1637
                     /locus_tag="VNU48_04930"
     CDS             468..1637
                     /locus_tag="VNU48_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWI10653.1"
                     /translation="MIDDVAIIGIGMHPFGRTDGLSGLEQGAHAVRLALQDAGLAWDA
                     VGVAYGGSQDAGNADALANRLGLTGTPFVNVANGCATGGSALASVVAAIRSGVAEIGV
                     AVGFDKHARGAFNARPKDWGLEDWYGATGLMLTTQFFALKTQRYLHEHGLSERALVLA
                     AQRSFRNGALNPQAWRRRELSIAEIAESMPVNLPLRKYMFCSPAEGGAALVLCSAKAA
                     HQYTRTPVLVKAALMRTRRHGSFEVFSPALAAEAAPAPTVFAARAAFEQAGVGPADID
                     VIQIQDTDSGSELIHMAENGFCEHGEQQRLLERGETDIGARLPINTDGGCMANGEPVG
                     ASGLRQVIEVCTQLRGQAGARQVPGRPRLGYTHVYGAPGVSAVTILQAGQAGDRR"
     gene            1634..3025
                     /locus_tag="VNU48_04935"
     CDS             1634..3025
                     /locus_tag="VNU48_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWI10654.1"
                     /translation="MNKPHEKQIFFQPPETAGDPLKFQQLLAQETVPVPAYLQSSPPQ
                     FGDEDLDVKRYTSHAFHRLEAEKMWTKAWQFVAREEEIPNAGDHVVYDIVDSSVILMR
                     GEDGAIRGFYNSCLHRGRTLRTQSGTTGQIKCPFHGFTWDIQGRFKSKPCSWDFKHLD
                     DRPLDLPQVQVQSWAGFVFINFDLQAEALLDYLGVLPEHFKSYLMERSTTIAHVQRRI
                     ACNWKVGQEAFFEAMHTKATHPGIMTFTGDVDSQYDVFGDIVARAITPMGVVSPNLGG
                     VDDTTTLNDILALSGRMSETDGSRHRLPEGVSARRYVGELNRQAYAAAAGADLSQATL
                     AELEDAILYSVFPNFQVWAGYFGNIVYRFIPDGDDTDHCLFDVYLLGRHPQGGACPPA
                     APLHRLGDDEPFTNAPQIGALGPVFEEDMRNLPHMMKGMRASQRGKVSLASYQESRIR
                     HHHLTLDKFLGLA"
     gene            3022..4038
                     /locus_tag="VNU48_04940"
     CDS             3022..4038
                     /locus_tag="VNU48_04940"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWI10655.1"
                     /translation="MTHNRQWILRERPPGEVTGRHLQLRRVAKPVPAAGEVLVRNIYL
                     LVPPAMRLWMNPQDSYVPAQPLGEPMMGITLGVVEASNDPALPVGRCVNGMGGCQEWF
                     TATAAQLMPLTPHPQVPLAAYRSVLDVQGLTAWAGLHEVCQPQPGQTLVVTAAAGSVG
                     SLVCQLGAQLGLHVVGIAGGADKCRWLREDCGIAATIDYKAEDVGARLDALCPRGIDR
                     VFENVGGPVLDLLLERINDRARIALCGMVASYNGGGTQSAGALMRLVTKAARLEGFLV
                     RDHFHRHAEVAAKLQALVLEGRLQYRIDILDGLEQLTVALDNLFHGRNQGVQLLRLSP
                     EPRA"
     gene            4035..>4752
                     /locus_tag="VNU48_04945"
     CDS             4035..>4752
                     /locus_tag="VNU48_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI10656.1"
                     /translation="MSDGTAAVRARAERLRADIRHFCAEAMPVALRRKSERHQVLDKA
                     DYQQWMQLLQAQGWGVGHWPEVHGGAGWSTLERFAFEDELARHGCPWVIPFGVKYVGP
                     VIYTYGSEAQKQRFLPRIVNGEDFWAQGYSEPGAGSDLAALRTEAVRDGDHYRVSGQK
                     VWTTYAQWADWLFCLVRTNGAPRQPGTVGDPPGGCRPAWERPGARPARDGRPQDGISF
                     LLIDMRSPGITVKPIATMDGY"
BASE COUNT          760 a         1706 c         1609 g          677 t
ORIGIN      
        1 cataaacaaa cagacatcat cgttttcaat atgccagacc cgcaactgat cgccgctcac
       61 ctgctgcgcc tggacggcgc acagccggcg ctgctcggca gccgctgctg cgcctgtggc
      121 gaggtctact tcccggccgc acacggctgc acgcgctgct gcggcacgcg gctcgaggcc
      181 ttcgacctcg gccaccgcgg ccgcctgtgg tcgtggacgg tgcaggactt ctgccccaag
      241 ccgccctacg acggcgccga gaacgaagcc gacttccggt cttacggcgt gggctacgtg
      301 gagatggcct gcggcatcaa ggtcgagagc cggctgacgc tggccgaccc ggcgcggttg
      361 cacatcggca tggccatgga attgacgctc gatccgtacc gccaccacac ggacggaacg
      421 gccatccaca cttacgcctt ccggccgctg cgcgacgagg cctcgacatg atcgacgacg
      481 tcgccatcat cggcatcggc atgcacccgt tcgggcgcac cgacggcttg tcgggcctcg
      541 agcaaggggc gcatgcggtt cgcctggcgc tgcaggacgc cggcctggcg tgggacgccg
      601 tcggcgtggc ctacggcggc agccaggacg ccggcaatgc cgacgcgctg gccaaccggc
      661 tcggcctgac cggcacgccg ttcgtcaacg tcgccaatgg ctgcgccacc ggcggcagcg
      721 cgctggccag cgtggtggcg gcgatccgct cgggcgtggc cgagatcggc gtcgcggtcg
      781 gcttcgacaa gcatgcgcgc ggcgccttca acgcccgccc caaggactgg ggcctggaag
      841 actggtacgg cgccaccggg ctgatgctga cgacgcagtt cttcgcgctg aagacccagc
      901 gttacctgca cgaacacggc ttgagcgaac gtgcgctggt gctggcggcg cagcgctcgt
      961 tccgcaacgg cgcactcaac ccgcaggcct ggcgccgccg cgagctgagc attgccgaga
     1021 tcgccgaatc gatgccggtg aacctgccgc tgcgcaagta catgttctgc agcccggccg
     1081 aaggcggcgc ggcgctggtg ctgtgcagcg cgaaggcggc gcaccagtac acccgcacgc
     1141 cggtgctggt gaaggcggcg ctgatgcgca cgcgccggca cggctcgttc gaggtcttca
     1201 gcccggcgct cgctgccgag gctgcaccgg caccgacggt gttcgcggcc cgcgccgcct
     1261 tcgagcaggc cggcgtcggc ccggccgaca tcgacgtgat ccagatccag gacaccgaca
     1321 gcggctccga gctcatccac atggccgaga acggcttctg cgagcacggc gagcagcagc
     1381 gcctgctgga gcgcggcgag accgatatcg gcgctcgcct gccgatcaac accgacggcg
     1441 gctgcatggc caacggcgag ccggtcggcg cctccggctt gcgccaggtg atcgaggtct
     1501 gcacgcagct gcgcggccag gccggcgcac gccaggtgcc cggccgcccg cggctgggct
     1561 acacgcatgt ctacggcgcg cccggcgtca gcgcggtgac gattcttcaa gcaggacaag
     1621 caggagacag gcgatgaaca agccgcacga gaagcagatc ttcttccagc cgccggaaac
     1681 cgccggcgac ccgctgaagt tccagcagct gctggcgcag gagacggtgc cggtgccggc
     1741 ctatctgcaa tcttcaccgc cgcagttcgg cgacgaagac ctggacgtga agcgctacac
     1801 ctcgcacgcc ttccaccggc tggaagccga gaagatgtgg accaaggcct ggcagttcgt
     1861 cgcccgcgaa gaggagatcc cgaacgccgg cgaccacgtc gtctacgaca tcgtcgacag
     1921 ctcggtgatc ctgatgcgcg gcgaggacgg cgcgatccgc ggcttctaca actcctgcct
     1981 gcatcgcggc cgcacgctgc gcacgcaaag cggcaccacc gggcagatca agtgcccgtt
     2041 ccacggcttc acctgggaca tccagggccg cttcaagagc aagccctgca gctgggactt
     2101 caagcacctg gacgatcgac cgctcgacct gccgcaggtg caggtgcaga gctgggccgg
     2161 cttcgtcttc atcaacttcg acctgcaggc tgaagcgctg ctggactacc tgggcgtgct
     2221 gcccgagcac ttcaagtcct acctgatgga gcgctcgacc accatcgccc atgtgcagcg
     2281 ccgcatcgcg tgcaactgga aggtcggcca ggaagccttc ttcgaggcca tgcacaccaa
     2341 ggccacgcac ccgggcatca tgaccttcac cggcgacgtc gacagccagt acgacgtgtt
     2401 cggcgacatc gtcgcgcgcg ccatcacgcc gatgggcgtg gtcagcccga acctcggcgg
     2461 tgtcgacgac acgacgacgc tgaacgacat cctcgcgttg tcgggccgca tgtccgagac
     2521 cgacggctcg cgccaccgcc tgcccgaggg cgtgtcggcg cgccgctacg tcggcgagct
     2581 gaaccgccag gcctacgccg ccgcggccgg cgccgacctg tcgcaggcca cgctggccga
     2641 gctggaggac gcgatcctgt actcggtgtt cccgaacttc caggtctggg cgggctactt
     2701 cggcaacatc gtctaccgct tcattcccga cggcgacgac accgaccact gcctcttcga
     2761 cgtctacctg ctcggccgcc acccgcaggg cggcgcgtgc ccgccggccg cgccgctgca
     2821 ccgcctgggc gacgacgaac cgttcaccaa cgcgccgcag atcggtgccc tcggccccgt
     2881 gttcgaagaa gacatgcgca acctgccgca catgatgaag ggcatgcgcg cgagccagcg
     2941 cggcaaggtg agtctggcca gctaccagga aagccgcatc cgccaccacc acctgacgct
     3001 ggacaagttc ctcgggctgg catgacgcac aaccgccaat ggatcctgcg cgagcggccg
     3061 ccgggcgagg tgaccgggcg ccacctgcag ctgcgccgcg tcgccaagcc cgtgcccgcc
     3121 gccggcgaag tgctggtgcg caacatctac ctgctcgtgc cgccggcgat gcgcttgtgg
     3181 atgaacccgc aagacagcta cgtgccggcg cagccgctgg gcgagccgat gatgggcatc
     3241 acgctcggcg tggtcgaggc ctcgaacgac ccggcgctgc cggtgggccg ctgcgtcaac
     3301 ggcatgggcg gctgccagga gtggttcacc gcgaccgccg cgcagctgat gccgctgacg
     3361 ccgcacccgc aggtgccgct ggcggcctac cgcagcgtgc tcgacgtgca aggcctcacc
     3421 gcctgggccg gcctgcacga ggtctgccag ccgcagcccg gccagacgct ggtggtgacg
     3481 gccgccgccg gcagcgtcgg ctcgctggtc tgccagctcg gtgcgcaact cggcctgcac
     3541 gtcgtcggca ttgccggcgg cgccgacaag tgccgctggc tgcgtgaaga ctgcggcatc
     3601 gccgcgacca tcgactacaa ggccgaggac gtcggtgcgc ggctcgacgc gctgtgcccg
     3661 cgcggcatcg acagggtgtt cgagaatgtc ggcggcccgg tgctggacct gctgctcgaa
     3721 cgcatcaacg accgcgcccg catcgcgctg tgcggcatgg tggccagcta caacggcggc
     3781 ggcacgcaga gcgccggtgc gttgatgcgt ctggtgacca aggccgcgcg tctggaaggc
     3841 ttcctggtgc gcgaccattt ccaccgtcat gccgaggtgg cagcgaagct gcaggcgctg
     3901 gtgctcgaag gccggctgca gtaccgcatc gacatcctcg acggcctgga gcaattgacc
     3961 gtcgcgctgg acaacctgtt ccatggccgc aaccagggcg tgcagctgct gcgcctgtcg
     4021 ccggagcctc gcgcatgagc gatggaacgg cagccgtccg cgcgcgcgcc gagcgcttgc
     4081 gcgccgacat ccgccacttc tgcgccgagg ccatgcccgt cgcgctgcgc cgcaagtccg
     4141 agcgccacca ggtgctggac aaggccgact accagcagtg gatgcaactg ctgcaggcgc
     4201 agggctgggg ggtcggccac tggcccgagg ttcacggcgg cgccggctgg tcgacgctgg
     4261 agcgtttcgc gttcgaggac gaactggcgc gccacggctg cccctgggtg attccgttcg
     4321 gcgtgaagta cgtcggcccg gtcatctaca cctacggcag cgaagcgcag aagcagcgct
     4381 tcctgccgcg catcgtcaat ggcgaagact tctgggccca gggctactcc gagcccggcg
     4441 ccggctccga cctggccgca ctgcgcaccg aggccgtgcg cgacggcgac cactaccgcg
     4501 tcagcggcca gaaggtctgg accacttacg cgcaatgggc cgactggctg ttctgcctcg
     4561 tccgaacgaa tggcgcccct cgccaaccgg gtacggttgg cgatcccccg gggggatgcc
     4621 ggccggcttg ggagcggccc ggcgctcggc cggctcgcga tgggcggccg caggacggca
     4681 tctccttcct gctcatcgac atgcgcagcc ccggcatcac cgtgaagccg atcgccacga
     4741 tggacggcta tc
//
LOCUS       DATMJB010000223         3703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_399581, whole genome shotgun sequence.
ACCESSION   DATMJB010000223 DATMJB010000000
VERSION     DATMJB010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399581"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VNU48_04950"
     CDS             <1..771
                     /locus_tag="VNU48_04950"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWI10657.1"
                     /translation="YRLAAFDNIGSTNAEAMARGRDGERGPMWFVTSEQTAGRGRRSR
                     PWIAPRGNLASSIFEVIDVSPSVAATLGFAAGLALEAALQRVSVEASLRAAGSDHMNY
                     ALKWPNDVLAGKQKLAGILLEAEAVGDGLAVVVGIGTNVVAAPEGTPTPATSLAAIGV
                     NIGAEELFSELSDSWARYRGVWDNGRGFGEIRRLWLERAAGLGQVVAIQSGGSTVEGI
                     FDTIDEAGSLVVRTPDGKRVPISAGDVYFGSAKSAGAA"
     gene            771..2441
                     /locus_tag="VNU48_04955"
     CDS             771..2441
                     /locus_tag="VNU48_04955"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWI10658.1"
                     /translation="MAQPDELTFAPLGGVGEIGMNLSIYGLGNRHQRSWLAIDLGVSF
                     GDEEHLPGIDLIMPDIGFLEKERKNLVGLVLTHAHEDHFGAIIDLWPKLKCPIYATKF
                     SAALFEAKCNSERNPPKIPVTVVPSGGRIDLGPFNVEFIPVAHSIPESHALAIHTSAG
                     TVLHTGDWKIDPTPIIGKPTDERRLRELGDAGVLALVGDSTNAVREGRSPSETEVART
                     ITELVKAAKGRVAVTTFASNVARLRAVADAAKAAGREVVVVGRAMERVVQVARETGHL
                     DGVQNFRGADLYGHFPPDKVLALCTGSQGEPRAALARIANDDHPQVTLNKGDCVIFSS
                     RTIPGNEKSVGAIINGLITQGVEVITDRTHLVHVSGHPRRDELRDMISWVRPQLLIPV
                     HGEALHLFEHAKLARAAGVPKVLICRNGDLVKLGPGDPGVIEELPSGRLYKDGRILED
                     SKSRAVTERRKLAFAGCAFVAIAVNEKGELADDPEIDLVGIPEKNTAGEVIDGIVFDV
                     VVSTVEGLPRARRRDPDAMAESVRRAVRSAVNEQWGKKPLCYVHVLVV"
     gene            2466..2870
                     /gene="mce"
                     /locus_tag="VNU48_04960"
     CDS             2466..2870
                     /gene="mce"
                     /locus_tag="VNU48_04960"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWI10659.1"
                     /translation="MLGRLNHVAIAVKDAEKAAKIYGGAFGAEISGAVPLPEHGVITV
                     FVTLPNTKIEFIQPLGDASPIAKFVERNADGAIHHICYDVPDIIGARDTLIAQGARVL
                     GDGVPKIGAHGKPVLFFHPKDFSGALVEIEQA"
     gene            2870..3145
                     /locus_tag="VNU48_04965"
     CDS             2870..3145
                     /locus_tag="VNU48_04965"
                     /inference="COORDINATES: protein motif:HMM:NF018978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HWI10660.1"
                     /translation="MAYSISTAIAIYFVLWWIVLFVTLPFGVRSQHEDGEGAPGTDPG
                     APIVSRMRAKLIWTTIISAVIFGLSMAAYRADYFNIERLSKLMGTPF"
     gene            3249..3602
                     /locus_tag="VNU48_04970"
     CDS             3249..3602
                     /locus_tag="VNU48_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10661.1"
                     /translation="MTFQRTIAGLLILTIAGLAAAFYMNWKMLTRLRTVTAFVEGYVF
                     AEAVAACEKAQSTTPFKWNTGTRTSIVGLNSVKLDKNTVIASYTLLADGVYCDYDPIK
                     KKADIGSNFLERIEF"
BASE COUNT          681 a         1138 c         1220 g          664 t
ORIGIN      
        1 taccggctcg ctgccttcga caatattggc tcgaccaatg cggaagccat ggcgcgcgga
       61 cgcgatggcg agcgcgggcc gatgtggttc gtgacatcgg agcagacggc gggacgcgga
      121 cggcgaagcc gcccctggat cgcgccacgt ggtaatctcg ccagcagcat cttcgaggtg
      181 atcgacgttt cgccatcggt ggcggcgacg ctgggttttg cggcagggct tgccctggag
      241 gcggcgctgc aacgggtaag cgtcgaggcg tcgctgcgag cggcgggatc ggaccacatg
      301 aactatgcgc taaaatggcc caatgacgtg ctggcaggga agcaaaagct tgccggcatc
      361 ctgctggagg cggaggcggt cggggacgga ctggctgtcg tggtcggaat cggcaccaac
      421 gtggtcgcag cccccgaggg cacgccgaca ccggcgacct cgctggcggc catcggcgtc
      481 aatatcggcg cggaagagct gttctctgaa ctgtcggact cctgggcgcg ataccgcggc
      541 gtctgggaca atggccgcgg ctttggcgaa atccggcggc tctggctgga gcgcgcggcc
      601 gggcttgggc aggtcgttgc aatccagtcc ggcggttcaa ccgtggaagg gatttttgac
      661 accatcgatg aggccggcag tctggtggtc cggaccccgg acggcaagcg cgtgccgata
      721 tcggcgggcg acgtctattt cggctcggcc aagtcggcgg gggcagcctg atggcgcagc
      781 cggatgaact gacctttgca ccgctcggcg gtgtcggcga gatcggcatg aacctgtcga
      841 tctatggcct cggcaatcgc caccagcgtt cctggctcgc gatcgatctc ggtgtctcct
      901 tcggcgacga ggagcatctg cccggcatcg atctgatcat gcccgatatc ggctttctgg
      961 agaaagagcg gaagaatctg gttggcctcg tgctgaccca cgcccacgaa gaccatttcg
     1021 gcgccatcat cgacctctgg cccaagctga aatgcccgat ctatgccacc aagttcagcg
     1081 ccgcgctgtt cgaggccaaa tgcaattccg aacgcaatcc gccgaaaatc ccggtcaccg
     1141 tcgtgccatc gggcggtcgc atcgatctcg gcccgttcaa tgtcgagttc attccggtcg
     1201 cgcattcgat tccggaatcg catgcgctgg cgatccacac ctcggccgga accgtgctgc
     1261 ataccggcga ctggaagatc gatccgacgc cgatcatagg taagccgacc gacgaacggc
     1321 ggttgcgcga actcggcgat gccggtgtgc tggcactggt cggcgattcc accaacgccg
     1381 tgcgggaagg gcgctcgccg tccgagaccg aagtcgccag gaccatcact gagctggtga
     1441 aggccgccaa gggccgcgtc gcggtcacca cctttgcttc caatgtcgcg cggctgcggg
     1501 cggtcgccga tgcggcgaag gccgcgggcc gcgaggtcgt ggtggtcgga cgcgcgatgg
     1561 agcgcgtggt gcaggtcgcg cgcgaaaccg gccatctcga cggcgtgcag aattttcgcg
     1621 gcgccgatct ctacgggcac ttcccgccgg acaaagtgct ggcgctgtgc accggcagcc
     1681 agggcgagcc gcgcgcggcg ctggcgcgca tcgccaatga cgaccacccg caggtgacgc
     1741 tcaacaaggg cgattgcgtg atcttttctt cgcgcaccat tcccggcaac gaaaagtcgg
     1801 tcggcgccat catcaacggc ctgatcaccc agggcgtcga ggttatcacc gaccgcaccc
     1861 atctggtcca cgtctccggc catccccgcc gcgatgaact gcgcgacatg atttcgtggg
     1921 tacggccgca actcctgata cccgtgcacg gcgaggcgct gcatctgttc gagcatgcca
     1981 agctggcgcg tgctgccggc gtgcccaagg tgctgatctg ccgtaacggc gatctggtta
     2041 aactgggccc gggcgatccc ggcgtgatcg aggaactgcc gtcggggcga ctctacaagg
     2101 acggcaggat ccttgaggat tccaaatcgc gcgcggtcac cgagcggcgc aagctggcct
     2161 ttgccggctg cgccttcgtg gcgattgccg tgaacgagaa gggcgaactg gccgacgatc
     2221 ccgagattga tctagtcggt atacccgaga aaaataccgc cggcgaggtg atcgacggga
     2281 tcgtgttcga cgtggtggta tcgaccgtgg aaggtttgcc gcgcgcgcgg cggcgcgatc
     2341 ccgacgcgat ggcggaatcg gtgcgccggg cggtgcgttc ggccgtgaac gagcaatggg
     2401 gcaagaagcc cctgtgctac gtccatgttc tggtagtcta ggcaggattc aaacggagaa
     2461 gtttcatgct gggccggctc aatcatgtcg cgatcgcggt caaggacgcc gagaaggccg
     2521 cgaagatcta tggcggcgcg ttcggcgccg agatttcggg ggcggtgccg ctaccggagc
     2581 atggcgtcat caccgtgttc gtgacgctgc ccaacaccaa gatcgagttc atccagccgc
     2641 tcggcgacgc ttcgccgatc gccaaattcg tcgagcgcaa tgccgacggt gccatccacc
     2701 acatctgcta cgacgtgccc gacatcatcg gggcgcgcga caccctgatc gcgcagggcg
     2761 cgcgggtgct cggcgacggc gtgcccaaaa taggcgccca cggcaagccg gtgctttttt
     2821 tccacccgaa ggatttttcg ggcgcgcttg tcgaaatcga gcaggcctga tggcctacag
     2881 catctccact gcgatcgcga tttacttcgt cctgtggtgg atcgtgctgt tcgtgacgct
     2941 gccgttcggc gtccgcagtc agcacgagga cggcgagggc gcccccggca ccgaccccgg
     3001 tgcgccgatc gtaagccgga tgagagccaa gctgatctgg accacgataa tatcggcagt
     3061 catcttcggc ctctcgatgg cggcgtatcg tgcggactat ttcaatatcg agcggctctc
     3121 gaagctgatg ggaacgccgt tttagggctt gaaggactga tctcgcccgc gaccatctcg
     3181 cgccggacga ctgaagacgc gctttctcat tcgaaggata cgaagcggta gcttgccggg
     3241 agaacaaaat gacttttcaa cgaaccattg ccggactttt gatcctgacc atcgccggcc
     3301 tcgccgcggc gttctacatg aactggaaga tgttgacgcg gctgcgcacg gtgacggcct
     3361 tcgtcgaagg ctatgtattc gcggaagccg tcgcagcctg cgagaaggcg cagagcacga
     3421 ccccgttcaa gtggaatacc ggcacgcgga cctcgatcgt cggactgaac tcggtcaagc
     3481 tcgacaagaa taccgtcata gctagctaca cgctgctcgc ggacggcgtc tattgcgact
     3541 acgacccgat caagaagaaa gccgatatcg gctcgaattt cctcgagcgg attgagtttt
     3601 aggcgcccca atcaggcctc gctgtgcgcc tcagcctggg tgcggccatg atgtcgcttg
     3661 agaacgccgc aaaaaaaaga gcagggcgca agccctgctc taa
//
LOCUS       DATMJB010000224         5529 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_177724, whole genome shotgun sequence.
ACCESSION   DATMJB010000224 DATMJB010000000
VERSION     DATMJB010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177724"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VNU48_04975"
     CDS             <1..1396
                     /locus_tag="VNU48_04975"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HWI10662.1"
                     /translation="LDFVYKKNQGFRVSAASWYDAAYDDKVNGNPAYQAAGLGTAYPN
                     NRYTPAVSRYYLHSGEFLDAFAFGRVSLGADMPLDVKLGRHTVIYGEALFSPIHGVSY
                     SQGPVDFRKALATPGAEAKELFLPLNQLSAHLQINDKLSLNAQYFLEWKPYRIYEGGT
                     YFTYADPFFQGGTNYLGIPFNGDLKSGPDAIPKNRGNWGLNAKWSPDWLNGTAGLTLR
                     KFDDKVMGVLSPGGTFGELHNAYAKDVKLVGLSYATQLSGVSIGSELVYRKNTALNTS
                     FGSAGLARGDSVHFLVNALAYIGKTPLFDSSTLLAEVTYSKLQKLDASTAAYFSHVDH
                     NCPVGGKDAGCSTNSAWGLSLSATPTWFQAFPSVDLTMPISFSTGLKGNSPVPFGGNE
                     GSGQWSLGLGADVMGKYKFDIAYTDYFGHYTAGPNIFAAVPGIGPNQMAYSNGGNALL
                     KDRGWLSITFKTSF"
     gene            1417..2793
                     /locus_tag="VNU48_04980"
     CDS             1417..2793
                     /locus_tag="VNU48_04980"
                     /inference="COORDINATES: protein motif:HMM:NF018716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HWI10663.1"
                     /translation="MKLAKTQLTALIFGSLAASAFAAVTPEEAKMLATTHTPWGAEIA
                     GNKDGSIPAYSGLVKPPANYDTKSPGVRPDPFAAEKPLFSIDAKNLDKYADKVSDGTK
                     AMLKKYPGYRIDVYPSHRVAHYPPFVVENMKKNAAGACKTVKNGLGIEGCYAGLPFPF
                     PKSGNEVMWNRLLKYDTAAFYSPDMAGRVVDTQGNVTVTGVGEMWAEYPIYDPKRTTP
                     IGTDETFERLRIDYNDPARKAGEKLIVVDNIDMQDRGRRAYSYLPGMRRVKLAPDIAY
                     DTPSPTGGGAGTVDEAAVFYGSLDRYDFKLVGKRELYIPYNTYRIRDRNACPTAVAMK
                     TKNYLNPDCMRWELHRVWEVKATLKAGKRHVYPTRTIFWDEDLPGVGISDNYDSAGQI
                     YRVSHSLPISFYETYGHSTDEWVTYDLATGNYARQEDVSEKGGWVPTPFQPDSFFSPE
                     VLSGAGVR"
     gene            2815..4491
                     /locus_tag="VNU48_04985"
     CDS             2815..4491
                     /locus_tag="VNU48_04985"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HWI10664.1"
                     /translation="MDHALHHQVETSREVDTDLTVTSGTWPAGLWGHAFIIGPAQPTL
                     VNFCWAGFGIVTRVDLASGAAGVPHWTSRHVRTPDSELMAAALGAVPAAELPGLLAGA
                     QPAFTNTAPHFLGDRLLLTYDRQRPAEFDPQTLAFRSYVGGVADYPVVSPHPLFPGVQ
                     TTGHPVEDLDDGCMWWCNVGLEPMGRSRTDRQGPLQVVRWNGHGDIETWFVPGAAITQ
                     GIHEIAVTQDYVIFTEVGNQDEPGAFAGRGRTKPHPPYSDITIVAKRELTRARRGKAV
                     PCTHARLPYESFHEFADYRQDGDDITLYIAHSNGWDLNIALGESDRTWHGGRPIAPGL
                     HGFLPLPVDASPVGRYVIDGRTGEVKDTKRFVDPGRHWATLLYARDMRRPALERSRYL
                     WQAYWGYNPEMLVPQAVELHREHPYRIVPVEELSERTLPSTVACIDLDTMQEQSSWAF
                     PAGAFAQSPVFVPDRAGGDGWMLAFVQFPDHTELQVFDALALGKGPVATATAPGLKQS
                     FQVHSGWMPRLAPQPIDYRRSSLGADLGSAWRELPAALRAAVEPVVQYWS"
     gene            4497..>5529
                     /locus_tag="VNU48_04990"
     CDS             4497..>5529
                     /locus_tag="VNU48_04990"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HWI10665.1"
                     /translation="MPAIIESPARRRLCAALGLLPLTAGAFQPPHEIAAQPTLLAASS
                     PLLAVARAGSRIVAAGPRGHIVWSDDDGAHWNQASVPVSSDLLALSFASARHGWAVGH
                     GGVVLHSDDGGATWQRQLGGHQANVLAQRHLESRAATDAQAAKLLQREKAQAADGSTP
                     SFLDVHFDSETSGFVVGTFNRIFRTEDGGRSWTPWMDRTQNPNDLHFYAVRGSARGVF
                     LAGEQGMVWRLAPNGQRFEAVATPYKGTLFGLVATDVGTLLAYGMRGRLFRSDDEGRQ
                     WQAVAGVGTAGITAGTVLPGGAIVLVDQAGGIALSRDAGQRFEPVPALQPMPYFGVVA
                     LGAQRIGLVG"
BASE COUNT          927 a         1965 c         1824 g          813 t
ORIGIN      
        1 actcgacttc gtctacaaga agaaccaggg ctttcgcgtc tccgcggcga gctggtacga
       61 cgccgcttac gacgacaagg tgaacggcaa tccggcgtat caggcggccg ggctcggcac
      121 cgcgtacccg aacaaccggt acacgccggc ggtgtcgcgc tactacctgc actcgggcga
      181 gttcctcgat gccttcgcgt tcggccgcgt ctcgctcggc gccgacatgc cgctggacgt
      241 caagctcggt cgccacaccg tgatctatgg cgaggcgctg ttcagcccga tccatggcgt
      301 gtcgtactcg cagggcccgg tggactttcg caaggcgctg gccacgccgg gtgccgaggc
      361 caaggaactg ttcctgccgc tgaaccagct ctcggcccac ctgcagatca acgacaagct
      421 gtcgctgaat gcgcagtact tcctcgagtg gaagccctac cggatctacg agggcggcac
      481 ctacttcacc tacgccgacc cgttcttcca gggcggcacc aactacctcg gcatcccgtt
      541 caacggcgat ctgaagagcg ggccggatgc catcccgaag aaccgcggca actggggctt
      601 gaacgcgaaa tggtcgccgg actggctgaa cggcacggcg ggcctgacgc tgcgcaagtt
      661 cgacgacaag gtgatgggcg tgctcagccc gggcggcacg ttcggcgagt tgcacaacgc
      721 ctacgcgaag gacgtcaagc tcgtcggcct gagctacgcg acgcagctgt ccggcgtgtc
      781 gatcggctcg gaactcgtct accgcaagaa caccgcgctg aacacgagct tcggctcggc
      841 cggtctggcg cgcggcgaca gcgtgcactt cctggtgaac gcgctggcct acatcggcaa
      901 gacgccactg ttcgattcgt cgacgctgct ggccgaggtc acctacagca agctgcagaa
      961 gctcgacgcg agcacggccg cgtacttcag tcacgtcgac cacaactgcc cggtcggcgg
     1021 caaggacgcc ggctgctcga cgaacagtgc ctggggcctg tcgctgtcgg ccacgccgac
     1081 ctggttccag gcgttcccga gcgtggacct gacaatgccg atctccttca gcaccgggct
     1141 gaagggcaac tcgccggtgc cgttcggcgg taacgagggc agtggtcaat ggagcctggg
     1201 cctcggcgcc gacgtgatgg gcaagtacaa gttcgacatc gcctacacgg actatttcgg
     1261 ccactacacc gccgggccca acatcttcgc ggccgtgccc ggcatcggcc cgaaccagat
     1321 ggcctattcg aacggcggca atgcgctgct caaggacagg ggttggttgt ccatcacctt
     1381 caagacgtcg ttctgatcct tcccaggaga cactgcatga aactcgctaa aacccaactg
     1441 accgcactga tcttcgggag cctggccgcc tcggccttcg ccgcggtgac ccccgaagaa
     1501 gccaagatgc tggcgacgac ccacacgccc tggggcgcgg aaatcgccgg caacaaggat
     1561 ggatcgatcc ccgcatactc cgggctggtc aagccgccgg cgaactacga caccaagagc
     1621 ccgggcgtgc ggccggatcc gttcgccgcc gagaagccgc tgttctccat cgacgccaag
     1681 aacctcgaca agtacgccga caaggtcagc gacggcacca aggcgatgct gaagaagtac
     1741 cccggctatc gcatcgacgt ctacccgtcg catcgcgtcg cccactaccc gccgttcgtc
     1801 gtggagaaca tgaagaagaa cgcggccggc gcgtgcaaga ccgtcaagaa cggcctgggc
     1861 atcgagggct gctatgccgg gctgccgttc ccgttcccca agagcggcaa cgaagtgatg
     1921 tggaaccgcc tgctgaaata cgacaccgcg gccttctact cgcccgacat ggccggccgc
     1981 gtggtcgaca cgcaaggcaa cgttaccgtc accggtgtcg gcgagatgtg ggccgagtac
     2041 ccgatctacg accccaagcg caccacgccg attggcaccg acgagacctt cgagcggctg
     2101 cgcatcgact acaacgatcc ggcccgcaag gccggcgaga agctgatcgt ggtcgacaac
     2161 atcgacatgc aggacagagg ccggcgtgcc tactcttacc tgccgggcat gcggcgcgtc
     2221 aagctggcac cggacatcgc ctacgacacg cccagcccga ccggcggcgg tgccggcacg
     2281 gtcgacgagg cggcggtgtt ctacggctcg ctcgaccgct acgacttcaa gctcgtcggc
     2341 aagcgcgagc tctacatccc ctacaacacc taccgcatcc gcgaccgcaa cgcctgcccg
     2401 acggccgtgg cgatgaagac caagaactac ctgaacccgg actgcatgcg ctgggaactg
     2461 caccgggtct gggaagtgaa ggccacgctg aaggcgggca agcgccacgt ctacccgacg
     2521 cgcacgatct tctgggacga agacctgccc ggcgtcggca tctcggacaa ctacgactcc
     2581 gcgggccaga tctaccgcgt gtcgcacagc ctgccgatct cgttctacga gacctacggg
     2641 cattcgacgg acgaatgggt gacctacgac ctggccaccg gcaactatgc gcgccaggaa
     2701 gacgtcagcg aaaagggcgg ctgggtgccg actccgtttc agcccgactc gttcttctcg
     2761 cccgaagtgc tgagcggcgc cggcgtgcgc tgagaccggg ccgggagaag ccgcatggac
     2821 cacgcgctgc atcaccaggt agagaccagc cgcgaggtcg acaccgacct gaccgtgacc
     2881 tccggcacct ggccggccgg cctgtgggga catgccttca tcatcgggcc ggcgcagccc
     2941 acgctggtga acttctgctg ggccggcttc ggcatcgtca cccgggtcga cctggcgtcg
     3001 ggtgccgccg gggtgccgca ctggacgagt cgccatgtgc gcacgcccga cagcgagttg
     3061 atggccgccg cgctcggcgc cgtgcccgcg gccgaactgc ccgggctgct ggccggcgcg
     3121 cagccggcct tcaccaacac cgcgccgcac ttcctgggcg accggctgct gctgacctac
     3181 gaccggcagc gcccggccga gttcgacccg cagacgctgg cgttccgcag ctacgtcggc
     3241 ggcgtggccg actaccctgt cgtcagcccg catccgctgt tccccggcgt tcagaccacc
     3301 ggccacccgg tcgaggatct ggatgacggc tgcatgtggt ggtgcaacgt cggcctggag
     3361 ccgatgggcc gctcgcgcac cgaccgtcaa ggccccctgc aggtggtgcg ctggaacggc
     3421 cacggcgaca tcgagacctg gttcgtgccc ggcgccgcca tcacgcaagg cattcacgag
     3481 atcgccgtca cgcaggacta cgtgatcttc accgaggtcg gcaaccagga cgagcccggc
     3541 gcctttgccg gccgcggccg caccaaaccg cacccgccgt acagcgacat caccatcgtc
     3601 gccaagcgcg agctgacgcg cgcgcggcgc ggcaaggccg tgccctgcac gcacgcacgg
     3661 ttgccctacg agtcgttcca cgagttcgcc gactaccggc aggacggcga cgacatcacg
     3721 ctgtacatcg cgcattccaa cggctgggac ctgaacatcg cgctcggcga aagcgaccgc
     3781 acctggcacg gcggccggcc gatcgcgccc gggctgcacg gcttcctgcc gctgccggtg
     3841 gatgcgtcgc cggtggggcg ctacgtgatc gacggccgca ccggcgaggt caaggacacg
     3901 aagcggttcg tcgaccccgg tcggcactgg gccacactgc tgtacgcgcg cgacatgcgc
     3961 cgcccggcgc tggagcgcag ccgctacctg tggcaggcct actggggcta caaccccgag
     4021 atgctggtgc cgcaggcggt cgaactgcac cgcgaacacc cgtaccgcat cgtgccggtc
     4081 gaagagctgt cggaacgaac cttgccgtcg accgtggcct gtatcgacct cgacacgatg
     4141 caggagcagt cgtcctgggc cttcccggcc ggtgccttcg cgcagtcgcc ggtgttcgtg
     4201 cccgaccgcg cgggcggtga tggctggatg ctcgccttcg tgcagttccc cgaccacacc
     4261 gagttgcagg tgttcgacgc gctcgccctc ggcaaggggc cggtcgcgac ggccaccgca
     4321 cccggcctga agcaatcgtt ccaggtgcac tccggctgga tgccgcgcct ggcgccgcag
     4381 ccgatcgact atcgccgcag ctcgctgggc gccgacctcg gctccgcctg gcgcgagctg
     4441 ccggccgcgc tgcgggcagc ggtcgagccc gtggtgcagt attggtcatg aacgccatgc
     4501 ccgccatcat cgaatcgccg gcgcgtcggc gcctgtgtgc ggcgctgggc ctgctgccgc
     4561 tgaccgccgg tgcgttccag ccgccgcacg agatcgcggc gcagccgaca ctgctggccg
     4621 cgagcagccc gctgctggcc gtcgcccgcg ccggttcacg catcgttgcc gccggcccgc
     4681 gcggccacat cgtctggtcc gacgacgatg gcgcccactg gaaccaggcc agcgtgccgg
     4741 tgagcagcga cctgctggcg ctgtcgttcg cgagcgcgcg ccacggctgg gccgtcgggc
     4801 acggcggcgt cgtgctgcac agcgacgacg gcggtgccac ctggcaacgc caactcggcg
     4861 ggcaccaggc caacgtgctg gcgcagcgcc acctcgaatc gcgtgccgcc accgacgcgc
     4921 aggcggccaa gctgctgcag cgcgagaagg cgcaggcggc cgacggcagc acgccgagct
     4981 ttctggacgt gcacttcgac agcgagacca gcggctttgt cgtcggcacc ttcaaccgca
     5041 tcttccgcac cgaggacggc ggccgcagct ggacgccgtg gatggaccgc acgcagaacc
     5101 cgaatgacct gcacttctat gccgtgcgcg gcagtgcacg cggcgtcttc ctggccggcg
     5161 agcaaggcat ggtctggcgg ctggcgccga acggccagcg cttcgaggcg gtggccacgc
     5221 cgtacaaggg cacgctgttc ggcctggtcg ccaccgatgt cggcacgctg ctggcgtacg
     5281 gcatgcgcgg acggctgttc cgcagcgacg acgagggccg gcagtggcag gccgtcgccg
     5341 gtgtcggcac ggccggcatc accgccggca ccgtgctgcc ggggggtgcg atcgtgctgg
     5401 tcgaccaggc cgggggcatc gccctcagcc gtgatgccgg gcagcggttc gagccggtgc
     5461 cggcgctgca gccgatgccg tacttcggcg tcgtcgcgct cggcgcgcaa cgcatcggcc
     5521 tggtcggcg
//
LOCUS       DATMJB010000225         4484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_468327, whole genome shotgun sequence.
ACCESSION   DATMJB010000225 DATMJB010000000
VERSION     DATMJB010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468327"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VNU48_04995"
     CDS             complement(<1..228)
                     /locus_tag="VNU48_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10666.1"
                     /translation="MQPVNWVSIRARDGKALRHAQPCGCFNPRTRRETRATCTTPLSI
                     RHPKGFNPPARRERRATLQLGRHAGALLCFNP"
     gene            388..1323
                     /locus_tag="VNU48_05000"
     CDS             388..1323
                     /locus_tag="VNU48_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWI10667.1"
                     /translation="MPSPRPHRQAHVARGLRSLSAALALAFGAGAALSESDPTRVTEL
                     REQALAYESGSGVAKDAALAVARYCEAARLGDAISQFNLGWLYANGRGVARDDAQAAF
                     FLRLAADQGIEQAANVLRLMGEPDPVAARPDCMRDPTLVPPPQAEAPAPVPVVAPKPI
                     VALVGKIAPKFRVPQPLVLAIMKAESNFDSEALSPKNAQGLMQLIPETALRFQVGNAL
                     DPAQNIRGGVAYLRWLLAYFEGDVSLVAAAYNAGEGTVERYRGVPPYLETRAYVQRIL
                     AAVGAAAQPFDTQVVAPSPYLAEIRERQRARIVGR"
     gene            complement(1320..3797)
                     /locus_tag="VNU48_05005"
     CDS             complement(1320..3797)
                     /locus_tag="VNU48_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HWI10668.1"
                     /translation="MNADGDAPLAGVTWFAQRGWTPFAFQREVWAALAQGRSGLLHAS
                     TGSGKTYAVWFGALARAATLGVPTNATQAPPLGVLWLTPMRALAADTTRALLAPLADL
                     GLTWSVGMRTGDTPAAERARQDRRLPTVLVTTPESLSLMLTREHARDELAGIHTVIVD
                     EWHELMGNKRGVQVQLALARLKRWNPALAVWGLSATLGNLPEAMQVLLGKTEGTLVQG
                     RVDKAIVIDTLLPDQPGRFSWGGHLGKQMQLPVVAEIERSSTTLVFVNVRSQAEIWYQ
                     LLLAEKPEWAGLVALHHGSLDREVREWVELGLKEGRLKAVVATSSLDLGVDFLPVERV
                     LQIGSAKGVARLLQRAGRSGHAPGRASRITLVPTNTLELVEAVAARRAAQAGRIEKRA
                     TPAKPLDVLVQHLVTVALGGGFKAAPMLAELRSAHAYRQLTKAEFDWALAFVVQGGAS
                     LAAYPEYHRVRQVDGVYRVPDRGIARRHRLQVGTIVSDSVMQVKYLSGGRIGVVEEGF
                     IARLRAGDCFVFAGRTLEYIRTHDMTAYVRRADKPRGVVTSWAGAKMPLSNELADAVL
                     EVLAGAAQGDRIDAMGEPELEAARPMLQTQLRLSTLPTPKTLLIERFTSREGQHLFIY
                     PFAGRNAHIGLASLLAWRLAKDAPNTFSISINDYGLELLSAVPIAPAPLLDQTLFSDT
                     ELLHDVLASLNSSELARRRFREIARVAGLIFTGYPGQPKSTRQLQASSSLFYEVFAKY
                     DAGNLLLTQAQTEVLSQELDIARLSASLARMRAQRIAFVELSVPSPFALPLMVERFRE
                     KLTTEKLKDRLDRLLKEMERAADRAAA"
     gene            complement(3794..>4484)
                     /locus_tag="VNU48_05010"
     CDS             complement(3794..>4484)
                     /locus_tag="VNU48_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWI10669.1"
                     /translation="RAEPAAHAASSALDGFAWAAQATMFLLLGLLVSPHDMLLTLAPT
                     LAVAAVLIFVARPLAVWLCLWPMGFGARERLFIGWVGLRGAVPIVLALFPLIEKVPDS
                     YRFFNAAFAVVLTSLLLQGTTLGWVAKRLRVVLPPETPPPARRAVQGRLTLDADLPLA
                     DVFEFFQLPLPETGAATTLRDWLADRLARDHVEGDGIDWHGARFSVGSLREGRIERVA
                     LALAPRAKTGS"
BASE COUNT          645 a         1637 c         1567 g          635 t
ORIGIN      
        1 tggattgaaa cacagcagtg cgcccgcgtg gcggccgagc tgcagcgtcg cgcgcctctc
       61 acgacgcgcg ggtggattga aacccttcgg gtgcctgatc gacaatggcg tcgtgcaggt
      121 cgcgcgcgtc tcacgacgcg tgcgtggatt gaaacaaccg catggttgag cgtggcgtaa
      181 agccttgccg tcgcgcgcgc ggattgaaac ccagttgact ggctgcatcc cgcgacgacc
      241 accagtcgcg cgcggctaca accgctgttc ccgggtcgtc ggatgccttt gcccggaggg
      301 agcgcggcgg cgaccccccg gttttgggat cgacaaggcg cgccgcgcaa ggcatattcg
      361 cgctccggtt ccctggagag tcccgagatg ccatcgccac ggccgcatcg acaggcccac
      421 gtcgcgcggg gtctgcgctc gctgagcgca gcgctcgcgc tggcattcgg cgccggcgcg
      481 gcactgtcgg agtccgatcc gacccgcgtc accgagttgc gtgaacaggc gctggcctat
      541 gaatccggca gcggcgtggc caaggacgcc gcactggccg tggcccgcta ctgcgaggcg
      601 gcccggctgg gcgacgcgat ctcgcagttc aacctgggct ggctctacgc gaacgggcgc
      661 ggcgtggcgc gcgacgacgc gcaggccgcg ttcttcctgc gcctggccgc cgaccagggc
      721 atcgaacagg ccgccaacgt gttgcggctg atgggcgagc ccgatccggt ggccgcgcgg
      781 cccgactgca tgcgcgatcc gacgctcgtg ccgccgccgc aggcagaggc gccggcgccc
      841 gttccggtgg tcgcgccgaa gccgatcgtc gcgctcgtcg gcaagatcgc accgaagttc
      901 cgcgtgccgc agccgctggt gctggccatc atgaaggccg agtcgaactt cgacagcgag
      961 gcgctgtcgc cgaagaacgc gcagggcctg atgcagctga tccccgagac ggcgctgcgc
     1021 tttcaggtcg gcaacgcgct cgacccggcg cagaacattc gcggcggcgt ggcctacctg
     1081 cgctggctgc tggcctactt cgagggcgac gtgtcgctgg tcgccgcggc ctacaacgcc
     1141 ggcgagggca ccgtcgaacg ctaccgcggc gtgccgccgt acctcgagac gcgtgcgtac
     1201 gtgcaacgca tcctcgcggc ggtcggcgcg gcggcgcagc ccttcgatac gcaggtcgtc
     1261 gcgccgtcgc cgtatctggc cgagattcgc gaacggcagc gcgcgcgcat cgtcggccgc
     1321 taagctgcgg cccggtcggc cgcgcgttcc atctccttca gcagccggtc cagccggtcc
     1381 ttcagcttct cggtcgtgag cttctcgcgg aagcgttcga ccatcagcgg cagcgcgaac
     1441 gggctcggca cgctcagctc gacgaaggcg atgcgctgcg cgcgcatgcg cgccaggctc
     1501 gcgctcaggc gtgcgatgtc gagctcctgg ctcagcacct cggtctgcgc ctgcgtcagc
     1561 agcaggttgc cggcgtcgta tttcgcgaac acctcgtaga acagcgagct cgacgcctgc
     1621 agctggcgcg tgctcttcgg ctgccccggg tagccggtga agatcagccc ggcgacacgc
     1681 gcgatctcgc ggaagcgccg gcgtgcgagc tcgctgctgt tcaggctcgc cagcacgtcg
     1741 tgcagcagct cggtgtcgct gaacagcgtc tgatcgagca gcggcgcggg ggctatcggc
     1801 accgcgctga gcagttccaa cccgtagtcg ttgatcgaga tgctgaaggt gttcggcgcg
     1861 tccttcgcca gccgccacgc gagcaggctc gccaggccga tgtgggcgtt gcggccggcg
     1921 aacgggtaga tgaacaggtg ctggccttcg cgcgacgtga agcgctcgat cagcagcgtc
     1981 ttcggcgtcg gcagggtcga caggcgcagc tgtgtctgca gcatcggtcg cgccgcctcg
     2041 agttcgggct cgcccatcgc gtcgatccgg tcgccctgcg ccgcgccggc cagcacctcg
     2101 agcaccgcgt cggccagctc gttcgacaag ggcatcttcg cgccggccca gctcgtcacc
     2161 acgccgcgcg gcttgtcggc gcgccgcacg taggccgtca tgtcgtgggt gcggatgtac
     2221 tcgagcgtgc gcccggcgaa cacgaagcag tcgccggcgc gcagccgcgc gatgaagccc
     2281 tcctcgacca cgccgatgcg cccgccgctc aggtacttca cctgcatcac cgagtccgac
     2341 acgatggtgc cgacctgcag gcgatggcgc cgcgcgatgc cgcggtcggg cacgcggtag
     2401 acgccgtcga cttgtctgac gcggtggtac tcgggatacg ccgccaggct cgcgccgccc
     2461 tgcacgacga aggccagcgc ccaatcgaac tcggccttcg tcaactggcg ataggcatgc
     2521 gcactgcgca gctcggccag catcggcgcg gccttgaagc cgccgccgag cgcgaccgtc
     2581 accaggtgct gcaccagcac gtccagcggc ttcgccggcg tcgcacgctt ctcgatccgg
     2641 ccggcctgtg ccgcgcgccg cgccgccacc gcctcgacca gctcgagcgt gttcgtcggc
     2701 accagcgtga tgcggctcgc gcgacccggc gcgtggccgc tgcggccggc gcgctgcaac
     2761 agccgcgcga cgcctttggc cgagccgatc tgcagaacgc gctcgaccgg caggaagtcg
     2821 acgccgaggt ccagcgacga ggtcgcgacc accgccttca ggcgcccttc cttcaagccg
     2881 agctcgaccc attcgcgcac ctcgcggtcg agcgagccgt gatgcagcgc gaccaggccg
     2941 gcccattcgg gcttctccgc cagcagcagc tggtaccaga tctcggcctg cgagcgcacg
     3001 ttgacgaaca ccagcgtcgt gctcgagcgt tcgatctcgg cgaccaccgg caactgcatc
     3061 tgcttgccca ggtgcccgcc ccacgagaac cgacccggct gatcgggcag cagcgtgtcg
     3121 atcacgatcg ccttgtcgac gcgtccctgc accagcgtgc cctcggtctt gccgagcagc
     3181 acctgcatcg cctcgggcag gttgccgagc gtcgcgctca ggccccagac cgcgagcgcc
     3241 gggttccagc gcttcagccg cgccagcgcg agctgcacct gcacgccgcg cttgttgccc
     3301 atcagctcgt gccactcgtc gacgatcacc gtgtggatgc cggcgagttc gtcgcgggcg
     3361 tgctcgcgcg tcagcatcag gctcagcgac tcgggtgtcg tcaccagcac tgtgggcaag
     3421 cgccgatcct ggcgcgcacg ttcggccgcg ggcgtgtcgc cggtgcgcat gccgacactc
     3481 caggtgagcc ccaggtccgc gagcggcgcg agcaatgccc gcgtcgtgtc ggccgcgagc
     3541 gcgcgcatcg gcgtcagcca cagcacgccc agtggcggcg cttgcgtggc attggtcggc
     3601 acgccgagcg tggccgcgcg cgccagcgcg ccgaaccaca ccgcgtaggt cttgccggag
     3661 ccggtgctcg cgtgcagcag tccgctgcgt ccctgggcga gcgcagccca gacctcgcgc
     3721 tggaacgcga acggcgtcca gccgcgctgc gcgaaccagg tcacacccgc gaggggcgcg
     3781 tcgccgtccg cgttcacgac ccggtcttcg cgcgcggcgc cagcgccagc gcgacgcgct
     3841 cgatgcggcc ttcgcgcagg ctgccgacgc tgaagcgcgc gccgtgccag tcgatgccgt
     3901 cgccctcgac gtggtcgcgc gcgagcctgt cggcgagcca gtcgcgcagc gtggtcgcgg
     3961 cgccggtttc gggcaagggc agctggaaga attcgaacac gtcggccagc ggcaggtcgg
     4021 cgtccagcgt caggcgaccc tgcaccgcgc ggcgcgccgg cggcggggtc tcgggcggca
     4081 gcacgacgcg cagccgcttc gcgacccagc ccagcgtcgt gccctgcagc agcagcgagg
     4141 tgagcacgac cgcgaacgcg gcgttgaaga agcggtagct gtcgggcacc ttctcgatca
     4201 gcgggaacag ggccagcacg atcggcaccg cgccgcgcag gccgacccag ccgatgaaca
     4261 gccgctcgcg cgcaccgaag cccatcggcc acaggcacag ccagactgcc agcggccggg
     4321 cgacgaagat cagcaccgcg gccaccgcca gcgtcggcgc gagcgtgagc agcatgtcgt
     4381 gcggcgagac cagcaagccc agcagcagga acatcgtggc ctgcgcggcc cacgcgaagc
     4441 cgtcgagcgc cgacgatgcg gcatgcgcgg ccggctcggc gcgc
//
LOCUS       DATMJB010000226         5509 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_861683, whole genome shotgun sequence.
ACCESSION   DATMJB010000226 DATMJB010000000
VERSION     DATMJB010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861683"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VNU48_05015"
     CDS             complement(<1..437)
                     /locus_tag="VNU48_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10670.1"
                     /translation="MSERLFASYRYSAFISYASDDDAAWNSFVSCFTDELNLALGSRL
                     HMSLPQAHLSGDDPLTTGSISDELRKNIEDSFVMFVFVHDSYLLSGYCRQELEHFASL
                     YGAQGFRDRLYLIAMSEPAMNALMAREDWKRLCPFSDQVWLKF"
     gene            complement(434..1678)
                     /gene="grrM"
                     /locus_tag="VNU48_05020"
     CDS             complement(434..1678)
                     /gene="grrM"
                     /locus_tag="VNU48_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophane-forming radical SAM/SPASM peptide
                     maturase GrrM/OscB"
                     /protein_id="HWI10671.1"
                     /translation="MMAAQALLRWLPRHPPGSLARRTAPRFAPADFAPALRTRLLILQ
                     PTPFCNIDCDYCYLPGRDSTARMSLATIRRAAERLREDDLLGPELSVVWHAGEPLTLP
                     AAYFDDAIAVLHEVLGAGCTLSHSMQTNAMLVDDARCAMIRRHDVRIGVSVDGPAELH
                     DAHRRTRQGRGTHAQVLRGMHRLREHGIGFHAIAVVTAATLAQPDAFVDFFEAEGVHE
                     LGCNFDEAEGLHARSSLAGREAAHAAFVARLLERTSAPGARLRIREFAHARQLIAQGL
                     PSQAWRGRHSPDNAQTLPFALVSVAHDGGFGTFSPELLGQRSAEFDDFVFGNVHRESY
                     LEGAAGDAFLRAWQAIRRGTQACEAACAHFAFCGGGAPANKFYENGDFASTETLYCRS
                     VIKRPFDALLARLERARDEMPS"
     gene            complement(1675..1980)
                     /locus_tag="VNU48_05025"
     CDS             complement(1675..1980)
                     /locus_tag="VNU48_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10672.1"
                     /translation="MTKHTTASGRVLRRAWRRALVWIAAVGLLGAASQANAIVRLERM
                     ALEARVQTLRAAMSASTDGVASDGQSRAQTEPWANWSNWNNWNNWPNWGNWGNWLNR"
     gene            2128..2781
                     /locus_tag="VNU48_05030"
     CDS             2128..2781
                     /locus_tag="VNU48_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWI10673.1"
                     /translation="MQHTSPDPSDDALMAAYAGGDARAFEQLYARHQAGLYRFIRRLL
                     GAALNAQTDEVFQDTWLRVVQSRARWQPQGASFRTWLFTLAHHRVVDQWRRSGREVSI
                     DAFEGDRGAPWEPAIDASAAWQHWPAPAPAASHTEELAFWRRAGERLLGCLEQLPIPQ
                     RSAFLLHHDDGLALDEVADALGVGFETAKTRLRYAMSKLRTCMGAYLAPLATGGESR"
     gene            2778..3776
                     /locus_tag="VNU48_05035"
     CDS             2778..3776
                     /locus_tag="VNU48_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10674.1"
                     /translation="MNASHGRPDDERDAWLSAALRHAPDAKVGPPAALSDTILRQARA
                     AARDARAPRSALAAAWDWLTRPPVAAGFASLMVATLVGLIWWDRPMDETLVRPPAEAA
                     TPATRDTHERVVPPATASSEAGVRQAPAATSERALAAPKSERSRAPRAQPQSSASAAP
                     AAPAPFSDIRARAPIAPADAGAAEPRDEATAVASGALARPAAKAAAAAPAAAAQLRRE
                     DSAAAARPAALAALLESIAAQPERWSWQRGHARQPMTPALQGWLLQLDRATTGRWQND
                     AAAPAGATPLQLYRDGVPVATLHLGDDTIWLAPVSQAALPRAALAALKQALDDATR"
     gene            3934..>5509
                     /locus_tag="VNU48_05040"
     CDS             3934..>5509
                     /locus_tag="VNU48_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWI10675.1"
                     /translation="MEVANAIRVRGARTHNLKNIDLDIPRNQLVVITGLSGSGKSSLA
                     FDTLYAEGQRRYVESLSAYARQFLQLMDKPDVDVIEGLSPAISIEQKATSHNPRSTVG
                     TVTEIHDYLRLLYARAGTPYCPDHHLPLQAQSVSQMVDAALALPEGTRLMVVAPVVRD
                     RKGEFTDLFADMQSQGYVRFRVDGTAYEAAELPKLKKAEKHDIDVVIDRVKVVAGLQQ
                     RLAESFEAALRIADGRAIALEMDTDKAHLFSSKFACPVCSYSLAELEPRLFSFNSPVG
                     ACPTCDGLGHTTVFDADRVVAFPSLSLASGAVKGWDRRNAYTFSLLESVAKHYSFDLD
                     TAFEELPKSAQQVLLQGSGSEDIEFVYSAEGARGKQRVVKRAHPFEGILPNLERRFRE
                     TDSPAVREDLMRYQSAKPCPDCAGTRLRREARHVFLVDVAGDTREPIFRVEHFTLREC
                     LAYFESLKLQGAKAEIADKVVREIRSRLKFLNDVGLNYLSLDRNADSLSGGESQRIRL
                     ASQIGSGLTGVMYVLDE"
BASE COUNT          816 a         1963 c         1960 g          770 t
ORIGIN      
        1 aagaacttca gccagacctg gtcgctgaac gggcacaggc gcttccagtc ctcgcgcgcc
       61 atcagcgcgt tcatcgccgg ctcggacatc gcgatcaggt agagccggtc acgaaagccc
      121 tgcgccccgt acaggctcgc gaagtgctcg agctcctggc ggcagtagcc cgacagcaga
      181 tagctgtcgt gcacgaacac gaacatcacg aacgagtcct cgatgttctt gcgcagctcg
      241 tcgctgatcg agccggtcgt cagcgggtcg tcgccgctca ggtgcgcctg cggcaggctc
      301 atgtgcaggc gcgagccgag ggcgagattg agttcgtcgg tgaagcacga gacgaacgag
      361 ttccaggccg cgtcatcgtc cgatgcgtag ctgatgaacg cgctgtagcg gtagctcgcg
      421 aagaggcgtt cgctcatgac ggcatctcgt cgcgcgcgcg ttccaggcgc gccagcagcg
      481 cgtcgaacgg gcgcttgatc acgctgcggc aatacagcgt ctcggtgctg gcgaagtcgc
      541 cgttctcgta gaacttgttg gccggcgcgc cgccgccgca gaacgcgaag tgcgcacagg
      601 ccgcctcgca ggcctgcgtg ccgcgccgga tcgcctgcca ggcgcgcagg aacgcgtcac
      661 cggccgcgcc ttcgagatag ctttcgcgat gcacgttgcc gaacacgaag tcgtcgaact
      721 cggccgagcg ctggccgagc agctcgggcg agaacgtgcc gaagccgccg tcgtgcgcga
      781 cgctgacgag cgcgaacggc agcgtctgcg cgttgtcggg cgagtgccgg ccgcgccatg
      841 cctgactcgg caggccctgc gcgatcagct gccgcgcgtg cgcgaattcg cggatgcgca
      901 gccgggcacc cggcgcgctg gtgcgttcga gcagccgcgc gacaaacgcg gcgtgcgcgg
      961 cctcgcggcc ggccaggctc gagcgcgcgt gcaggccttc ggcctcgtcg aaattgcagc
     1021 cgagttcgtg cacgccctcg gcctcgaaga agtcgacgaa ggcatcgggc tgcgccagcg
     1081 tcgccgcggt gacgaccgcg atcgcatgga acccaatgcc gtgctcgcgc agccggtgca
     1141 tgccgcgcag cacctgggcg tgcgtgccgc ggccctggcg cgtgcggcgg tgcgcgtcgt
     1201 gcagttcggc cgggccgtcg acactgacgc cgatgcgcac gtcgtggcgc cggatcatcg
     1261 cgcagcgcgc atcgtcgacc agcatcgcgt tggtctgcat cgagtgcgac agcgtgcagc
     1321 ccgcaccgag cacctcgtgc agcaccgcga tcgcgtcgtc gaagtacgcg gccggcagcg
     1381 tgagcggctc gcccgcgtgc cagacgacgc tgagttcggg gccgagcagg tcgtcctcgc
     1441 gcaaccgctc ggccgcgcgg cggatcgtcg ccaggctcat gcgcgcggtc gagtcgcgac
     1501 ccggcaggta gcagtagtcg cagtcgatgt tgcagaacgg cgtgggctgc aggatcagca
     1561 gccgggtgcg cagcgcgggc gcgaagtcgg cgggcgcgaa gcgcggcgcg gtgcggcgcg
     1621 ccaggctccc cggcgggtgg cgcggaagcc agcgcagcag cgcctgcgcg gccatcatcg
     1681 gttcaaccag ttgccccaat tgccccagtt gggccaattg ttccagttgt tccagttgct
     1741 ccagttcgcc cagggctcgg tctgtgcgcg agattgtccg tcgctcgcga cgccatcggt
     1801 cgaagccgac attgccgcgc gcagcgtctg cacgcgcgct tcgagcgcca tgcgttcgag
     1861 gcggacgatc gcgttcgcct gcgatgccgc gcccagcagg ccgaccgcgg cgatccagac
     1921 cagcgcgcgg cgccaggcgc ggcgcaaaac ccggccgctc gctgtcgtgt gcttcgtcat
     1981 caacggttcc aacgaatgtg gcttttcgat gttaggccgc cggtatcggc atgggaaggg
     2041 tcggcccctc cctagaaagc gcgctgcgat caccccgttc gatgggcccg aacgtatcag
     2101 ccatggatca tcaggcagac ttcccgcgtg cagcacacga gccccgaccc cagcgacgac
     2161 gcattgatgg cggcctacgc cggcggcgac gcgcgcgcgt tcgagcagct ctacgcgcgg
     2221 catcaggcgg gcctgtaccg cttcatccgg cgcctgctcg gcgcggcgct gaacgcgcag
     2281 accgacgagg tgttccagga cacctggctg cgcgtcgtgc agtcgcgcgc gcgctggcag
     2341 ccgcagggtg cgagctttcg cacctggctg ttcacgctgg cgcaccaccg cgtcgtcgac
     2401 cagtggcggc gcagcggccg cgaggtctcg atcgatgcgt tcgagggcga ccgcggcgcg
     2461 ccctgggaac ccgcgatcga tgcgtcggcc gcgtggcagc attggccggc gcccgcgcct
     2521 gccgcgtcgc acaccgagga gctcgcgttc tggcgccgcg ccggcgagag gctgctcggc
     2581 tgcctggagc agctgccgat cccgcagcgc agcgccttcc tgctgcacca cgacgacggc
     2641 cttgcgctcg acgaggtcgc cgacgcactc ggcgtcggtt tcgagaccgc gaagacgcgg
     2701 ctgcgctacg cgatgagcaa actgcgcacc tgcatgggcg cctatctggc gccgctggcc
     2761 acgggcggcg aatcgcgatg aatgcatcgc acggtcgtcc cgacgacgag cgcgacgcct
     2821 ggctcagcgc cgcgctgcgc cacgcgcccg atgcgaaggt cgggccgccg gccgcgctca
     2881 gcgacacgat cctgcggcag gcgcgtgccg cggcgcggga tgcgcgcgcg ccgcgcagcg
     2941 ccttggcggc ggcctgggac tggttgacgc ggccgccggt ggccgccggc ttcgcgagcc
     3001 tgatggtcgc gacgctggtc ggcctgatct ggtgggaccg gccgatggac gagaccttgg
     3061 tgcgcccgcc ggccgaggcc gcgaccccgg cgacgcgcga cacgcacgag cgcgtcgtgc
     3121 caccggccac ggcgagcagc gaggccggcg tgcgccaggc gcccgccgcg accagcgagc
     3181 gcgcgctcgc ggcacccaag tccgagcgct cgcgcgcgcc gcgcgcacag ccgcaatcat
     3241 ccgcatcggc cgcgcccgcg gcgccggcac cgttctcgga catccgtgcg cgcgcgccga
     3301 tcgcgccggc cgacgcgggc gcggccgagc cgcgcgacga agcgaccgcg gtggcatcgg
     3361 gtgcgctcgc gcgcccggcc gcgaaggcag cagcagcggc gccggccgcg gcggcccagc
     3421 tgcgccgtga agacagcgcc gcggctgccc gtcctgccgc gctggccgcg ctgctcgagt
     3481 cgatcgccgc gcagcccgag cgctggagct ggcagcgcgg ccacgcgcgc cagccgatga
     3541 ccccggcgct gcagggctgg ctgctgcagc tcgaccgtgc gacgacgggt cgctggcaga
     3601 acgacgccgc agcaccggcc ggtgccacgc cgctgcagct gtaccgcgac ggcgtaccgg
     3661 tggcgacgct gcacctgggc gacgacacga tctggctcgc gcccgtgtcg caggccgcgc
     3721 tgccgcgggc cgccctcgcg gccttgaagc aggcgctgga cgacgcgacc cgctgagcgc
     3781 gccgcgggtg gggtcggccc ggcgtggaac gaccggccgc tatcatggtc ggttttgcgc
     3841 gaaccaccga tgcccgaccc cgccgaatcc tccaccgcga acgcgagtcg cgcgccggcc
     3901 gcggaagtcg cgaacgcgat tcgcgtcccg tccgtggaag tcgcgaacgc gattcgcgtt
     3961 cgcggtgcgc gcacccacaa cctgaagaac atcgacctcg acatcccgcg caaccagctg
     4021 gtcgtgatca ccgggctgtc gggttcgggc aagagctcgc tcgcgttcga cacgctgtac
     4081 gccgagggcc agcgccgcta cgtcgagagc ctgtcggcgt atgcgcgcca gttcctgcag
     4141 ctgatggaca agcccgatgt cgacgtgatc gagggcctgt cgccggcgat cagcatcgag
     4201 cagaaggcga cctcgcacaa cccgcgctcg accgtgggca ccgtcaccga gatccacgac
     4261 tacctgcgcc tgctgtacgc gcgcgccggc acgccttact gccccgatca ccacttgccg
     4321 ctgcaggcgc agagcgtgag ccagatggtc gacgccgcgc tcgcgctgcc cgagggcaca
     4381 cggctgatgg tcgtcgcgcc ggtggtgcgc gaccgcaagg gcgaattcac cgatctgttc
     4441 gccgacatgc agtcgcaggg ctatgtgcgc tttcgcgtcg acggcaccgc ctatgaagcc
     4501 gcggagctgc cgaaactgaa gaaggccgag aagcacgaca tcgacgtcgt gatcgaccgc
     4561 gtcaaggtgg tggccggcct gcagcaacgg ctggccgaga gtttcgaagc ggcgctgcgc
     4621 atcgccgacg gccgcgcgat cgcgctcgag atggacaccg acaaggcgca cctgttctcc
     4681 agcaagttcg cctgcccggt ctgcagctac tcgctcgccg agctcgagcc ccggctgttc
     4741 tcgttcaact cgccggtcgg tgcctgcccg acctgcgacg gcctgggcca cacgacggtg
     4801 ttcgacgccg accgcgtggt cgcgttcccg tcgctgagcc tggctagcgg cgcggtgaag
     4861 ggctgggacc ggcgcaacgc ctacaccttc tcgctgctgg aaagcgtggc gaagcactac
     4921 agcttcgacc tcgacacggc gttcgaggag ctgccgaaga gcgcgcagca ggtgctgctg
     4981 cagggctcgg gcagcgagga catcgagttc gtctacagcg ccgagggggc gcgcggcaag
     5041 cagcgcgtcg tcaagcgcgc gcacccgttc gagggcatcc tgccgaacct ggaacggcgc
     5101 tttcgcgaaa ccgattcacc cgcggtgcgc gaggacctga tgcgctacca gagcgcgaag
     5161 ccctgtcccg attgcgccgg cacgcggctg cggcgcgagg cgcgtcatgt gttcctggtc
     5221 gacgtggccg gagacacgcg cgaacccatc ttccgcgtcg agcacttcac gctgcgcgaa
     5281 tgcctggcct acttcgagtc gctgaagctg cagggcgcga aggccgagat cgccgacaag
     5341 gtcgtgcgcg agatccgctc gcggctgaag ttcctcaacg acgtggggct gaactacctg
     5401 agcctggacc gcaacgccga ttcgctgtcg ggcggcgagt cgcagcgcat ccgcctcgcg
     5461 tcgcagatcg gctcgggcct gaccggcgtg atgtacgtgc tcgacgagc
//
LOCUS       DATMJB010000227         2223 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_782537, whole genome shotgun sequence.
ACCESSION   DATMJB010000227 DATMJB010000000
VERSION     DATMJB010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782537"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1342)
                     /locus_tag="VNU48_05045"
     CDS             complement(17..1342)
                     /locus_tag="VNU48_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HWI10676.1"
                     /translation="MTLPSPQTDPRGFLRALFDAAVAQAVPGPEIAAILPAPPDVAKG
                     GRTLVLGAGKAGGSMAAAVDAVWPRNAPMSGLVVTRYDYVPPAYKTLQQQGAGRIEVV
                     EAAHPVPDDAGRRAARRIAELTRGLTKHDLVLCLISGGASSLLAMPAEGITLEEKQTV
                     NKALLRSGAAIDEMNCVRKHLSAIKGGRLAAMCAPAQVVTLLISDVPGDSPQVIGSGP
                     TVPDTTTCADALRILERYAIEVPAAARAGLQSGAFETPKPGDARFEGHQVHMIATPQQ
                     SLAAAAALARTAGIGVHVLSDEMEGESREVGKVHAALARSVARHGQPFMKPCVILSGG
                     ETTVTVKTKGGRGGRATEFLLGCALALQGEPGVHVLAGDTDGIDGVEDNAGAFVTPST
                     LARAQGLGLKAQDFLDRNDAYDFFAPLGDLIVPGPTFTNVNDFRALLVV"
     gene            complement(1377..2219)
                     /locus_tag="VNU48_05050"
     CDS             complement(1377..2219)
                     /locus_tag="VNU48_05050"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HWI10677.1"
                     /translation="MIAGGHTLHVSSHHKAPDAELQAKAITVHPTPKAAAAASEIVIL
                     MLPDTPQVEEVLFGTNGVALGLNKGSIVVDMSSISPMATKDFAGRIEATGRFYLDAPV
                     SGGEVGAKAASLTIMVGGPDAAFERVKPLFELMGKNITLVGGNGDGQTCKVANQIIVA
                     LNIAAVGEALVFASKAGADPAKVRQALMGGFAASRILEVHGERMIKRTFAPGFRIGLH
                     QKDLSLALAGARALGVALPQTAGAAQLMQACAANGGQDLDHSSLVKALELMANHPVAP
                     DPAK"
BASE COUNT          299 a          804 c          785 g          335 t
ORIGIN      
        1 gacaccgcgc gcgggctcag acgaccagca gcgcgcgaaa gtcgttgacg ttcgtgaagg
       61 tcggccccgg cacgatcagg tcgccgagcg gcgcgaagaa gtcgtaggcg tcgttgcggt
      121 ccaggaaatc ctgcgccttc aaccccaggc cttgcgcgcg cgccagcgtg ctcggcgtga
      181 cgaacgcgcc ggcgttgtcc tcgacgccgt cgatgccgtc ggtgtcgccg gccagcacgt
      241 gcacgccggg ctcgccctgc agcgcgagtg cgcagccgag caggaactcg gtggcgcggc
      301 cgccgcggcc gcccttggtc ttgacggtca ccgtggtctc gccgccggag aggatcacgc
      361 acggcttcat gaacggctgg ccgtggcgcg cgacggagcg cgccagcgcg gcatgcacct
      421 tgccgacctc gcgcgactcg ccttccatct cgtcgctgag cacatgcacg ccgatgccgg
      481 ccgtgcgcgc cagcgcggcg gcggcggcca gcgactgctg cggcgtcgcg atcatgtgga
      541 cctggtgacc ttcgaagcgc gcgtcaccag gcttcggcgt ctcgaaggcg ccgctttgca
      601 gccccgcgcg tgcggccgcc ggcacctcga tcgcgtagcg ctcgaggatg cgcagtgcat
      661 cggcgcaggt cgtcgtgtcg ggcacggtcg gaccgctgcc gatgacctgg ggcgagtcgc
      721 ccggcacgtc gctgatcagc aaggtcacga cctgcgccgg cgcgcacatc gccgcgagcc
      781 ggccgccctt gatcgccgac aggtgcttgc gcacgcagtt catctcgtcg atcgcggcgc
      841 cggagcgcag cagcgccttg ttgaccgtct gcttctcctc gagcgtgatc ccctcggccg
      901 gcatcgccag cagcgacgac gcgccgcccg agatcaggca cagcacgagg tcgtgtttcg
      961 tcaggccccg cgtcagctcg gcgatgcgcc gggcggcgcg gcggccggcg tcgtcgggca
     1021 ccgggtgcgc ggcctcgacg acctcgatgc ggccggcgcc ctgttgttga agggtcttgt
     1081 aggccggcgg cacgtagtcg tagcgcgtga cgaccaggcc cgacatcggc gcgttcctcg
     1141 gccacacggc atcgacggcc gcggccatcg agccgccggc cttgccggca ccgagcacca
     1201 gcgtgcggcc gccctttgca acatcgggcg gcgccggcag gatcgcggcg atctccggcc
     1261 ccggcacggc ctgcgcgacc gcggcatcga acagcgcgcg cagaaagccg cgcgggtcgg
     1321 tctggggcga aggcagggtc atgcggcaat tctgccggcg cgcggccggc cgtcgttcac
     1381 ttcgcggggt cgggggcgac cgggtgattg gccatcagct cgagcgcctt gaccagcgac
     1441 gaatggtcga ggtcctgccc accgttggcg gcgcaggcct gcatcagctg cgccgcgccg
     1501 gcggtctgcg gcaacgcgac gccgagcgcg cgtgcgccgg ccagcgccag gctcaggtcc
     1561 ttctggtgca gcccgatgcg aaagcccggc gcgaacgtgc gcttgatcat gcgttcgcca
     1621 tgcacctcga ggatgcggct ggccgcgaag ccgcccatca gcgcctggcg caccttggcc
     1681 gggtcggcgc cggccttcga ggcgaacacc agcgcctcgc cgaccgccgc gatgttgagc
     1741 gcgacgatga tctggttcgc gaccttgcag gtctggccgt cgccgtttcc gccgaccagc
     1801 gtgatgttct tgcccatcag ctcgaacagc ggcttgacgc gctcgaacgc agcgtcgggg
     1861 ccgccgacca tgatcgtcag gctggccgcc ttcgcgccga cctcgccgcc ggagaccggt
     1921 gcatcgagat agaacctgcc ggtcgcctcg atccgccccg cgaaatcctt ggtcgccatc
     1981 ggcgagatcg agctcatgtc gacgacgatg ctgcccttgt tcaggccgag cgcgacgccg
     2041 ttggtgccga acagcacctc ctcgacctgc ggcgtgtccg gcagcatcag gatgacgatc
     2101 tcgctcgccg ccgcggccgc cttcggcgtc gggtggaccg tgatcgcctt cgcctgcagc
     2161 tcggcatccg gcgccttgtg atggctcgag acatgtagcg tatgaccgcc cgcgatcaga
     2221 tgt
//
LOCUS       DATMJB010000228         2996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_232409, whole genome shotgun sequence.
ACCESSION   DATMJB010000228 DATMJB010000000
VERSION     DATMJB010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232409"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..661)
                     /locus_tag="VNU48_05055"
     CDS             complement(212..661)
                     /locus_tag="VNU48_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10678.1"
                     /translation="METIAVFVNDAAAARRILQPMWRGEGPTHWVLVACPPALTRHIG
                     RWVTRSARHQWRERWAADLFAALAPESKLPAGSTLETLVAGRPLVAVSARLQARWPQL
                     RLLDARQQRVGRVDEPIGATPPASMADRWAAPAAATAGLAVVLTLAD"
     gene            complement(697..1389)
                     /locus_tag="VNU48_05060"
     CDS             complement(697..1389)
                     /locus_tag="VNU48_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWI10679.1"
                     /translation="MELFSAAWLSALLAIVLIDLVLAGDNAIVIALAARKLPRHLQTK
                     AIVWGTVGAIVVRTAMTVGVVWLLKIPGLMLVGGLGLVWIAYKLLVPGDAGATHEPGA
                     TNFWGAMKTIVIADALMGVDNVLGVAGAAKGSIDLVVIGLLISVPIVVWGSTLVLKLV
                     ERFAIITYLGAGVLAYTAAHMIVSEPLLDDLFDPSVAIRVATHALLVAGVLGAGWWAA
                     RRGAAERTPKTI"
     gene            complement(1621..2307)
                     /locus_tag="VNU48_05065"
     CDS             complement(1621..2307)
                     /locus_tag="VNU48_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HWI10680.1"
                     /translation="MDFSSGAYWAALGSIILANIVLSGDNAVVIAMAARSLPAHQQRR
                     AIFWGSGAAIVMRILLTLLAVEMLKWPYLKVVGALLLLYIGISLLGEDDGDDGHAKEI
                     GGMAAAIRTILVADLVMSLDNVLAVAAAAKGNTPLLIIGLAVSIPLIIFGSTLLLKVM
                     ERFPVIITLGAALLGYLAGEMLLTDPAVEQRFGEQSETAVNVAGAIGAAIVVAAGLWL
                     QQRTRDKTPA"
     gene            complement(2342..>2996)
                     /gene="sucD"
                     /locus_tag="VNU48_05070"
     CDS             complement(2342..>2996)
                     /gene="sucD"
                     /locus_tag="VNU48_05070"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWI10681.1"
                     /translation="AAAAIWEAVEADLDLAICITEGIPVKDMLMVRNRMKVKEAQGGK
                     KTLLLGPNCPGLITPDEIKIGIMPGHIHKAGRVGVVSRSGTLTYEAVAQLTELGIGQS
                     SAVGIGGDPINGLKHIDIMKLFNDDPNTDAVIMIGEIGGPDEADAARWCQKNMSKPIV
                     GFIAGVTAPAGKRMGHAGALISGGADTADAKLAVMEECGFTVTRNPSEMGKLLKGLL"
BASE COUNT          454 a         1068 c         1037 g          437 t
ORIGIN      
        1 ggccggcggc gcgcggcgcg caccgatgaa gcgcccgagc gcgaaggccg cgccgacaag
       61 cagcgcgatc gcgagccagc cgatccaacc cagtggcgac agcatcagcg cagccgccac
      121 gccgcggcgc aagggtggga agcgaggtgc ctgctcattg cgcaggcacg ttagcacagg
      181 cccgccgtac ggcggcgcgc ccggcgcggg ctcagtcggc cagcgtcagc accaccgcca
      241 gacccgccgt cgccgcggcc ggcgcggccc agcggtccgc catcgatgcg ggcggggtcg
      301 caccgatcgg ctcgtcgacg cggccgacgc gctgctggcg cgcgtcgagc aaccgcagct
      361 gcggccagcg cgcctgcaac cgcgccgaca cggcgaccag cggccggccc gcgaccagcg
      421 tttcgagcgt gctgcccgcc gggagcttcg actcgggcgc cagcgccgcg aacaggtccg
      481 ccgcccagcg ctcgcgccac tggtgccgcg cgctgcgcgt cacccagcgc ccgatgtggc
      541 gcgtcagcgc cggcgggcag gcgaccagca cccagtgcgt cggcccttcg ccgcgccaca
      601 tcggctgcag gatgcgacgc gccgcggcag cgtcgttgac gaacacggca atcgtttcca
      661 tggcgtctct ccagtggtct ggcggatctg tcggcgtcag atcgtcttgg gcgtgcgctc
      721 ggcggcgccg cggcgcgcgg cccaccagcc ggcgccgagc accccggcga ccagcagcgc
      781 gtgtgtggcg acgcggatcg cgacgctcgg gtcgaacaag tcgtcgagca gcggctcgct
      841 gacgatcatg tgggccgcgg tgtaggcgag cacgcctgcg ccgaggtagg tgatgatcgc
      901 gaagcgctcg accagcttca gcaccagcgt gctgccccag acgacgatcg gcacgctgat
      961 caacagcccg atcacgacca ggtcgatgct gcccttcgcg gcgccggcga cgccgagcac
     1021 gttgtcgacg cccatcagcg catcggcgat cacgatggtc ttcatcgcgc cccagaagtt
     1081 cgtcgcgccg ggttcgtgcg tcgcgccggc gtcgccgggc acgagcagct tgtaggcgat
     1141 ccagaccagg ccgaggccgc cgaccagcat cagtccgggg atcttcagca accagaccac
     1201 gccgaccgtc atcgcggtgc gcacgacgat cgcgccgacg gtgccccaga cgatggcctt
     1261 ggtttggaga tgccgcggca gcttgcgcgc ggccagcgcg atgacgatgg cgttgtcgcc
     1321 ggcgaggacg agatcgatca ggacgatggc cagcaaggcg gacagccagg ccgcagagaa
     1381 gagttccata gggtctccat gagcgttgca cgacggacga cgcgaagccg ctgcatggcg
     1441 cggctcgcgt cgaaggtctt gctcggcatg tggtgactct tctggcccgg caaaccggaa
     1501 gcaggtcgtg gaccccgctt cgtactgacg gcttgccgaa acacccgcgc ggccaaggcc
     1561 ggcgagcgtc gagctactcc ccttcggaac gccgattagg acagccgcag ctgacccgtg
     1621 tcaagccggg gtcttgtcgc gcgtgcgctg ctgcaaccac aggccggcgg cgacgacgat
     1681 cgccgcgccg atcgcgccgg ccacgttgac ggccgtctcc gactgctcgc cgaaacgttg
     1741 ctcgaccgcc gggtcggtca gcagcatctc gccggcgagg tagccgagca gcgccgcgcc
     1801 gagcgtgatg atgacgggaa agcgctccat caccttgagc agcaaggtgc tgccgaagat
     1861 gatcagcggg atgctgacgg ccaggccgat gatcagcagc ggcgtgttgc ccttcgccgc
     1921 ggcggcgacc gcgagcacgt tgtccaggct catcaccagg tcggcgacga ggatcgtgcg
     1981 gatcgccgcg gccatgccgc cgatctcttt cgcgtggccg tcgtcgccgt cgtcctcgcc
     2041 gagcagcgag atgccgatgt acagcagcag cagcgcgccg acgaccttca ggtacggcca
     2101 cttcagcatc tcgaccgcga gcagcgtcag caggatccgc atcacgatcg ccgccccgct
     2161 gccccagaag atcgcgcgtc tctgctggtg cgccggcagc gagcgcgccg ccatcgcgat
     2221 cacgacggcg ttgtcgcccg agagcacgat gttggcgagg atgatcgagc cgagtgccgc
     2281 ccagtaggcg ccgctgctga agtccatgcg tcttgaccgt gccgagggtc ggtgcgccgg
     2341 actagagcag gcccttgagc agcttgccca tctccgacgg gttgcgcgtg acggtgaagc
     2401 cgcactcctc catcaccgcg agcttggcat cggccgtgtc ggcgccgccc gagatcagcg
     2461 caccggcgtg gcccatgcgc ttgcccgcgg gcgccgtcac gccggcgatg aagccgacga
     2521 tgggcttgct catgttcttc tggcaccagc gcgccgcatc ggcttcgtcg gggccgccga
     2581 tctcgccgat catgatcacc gcgtcggtgt tcggatcgtc gttgaacagc ttcatgatgt
     2641 cgatgtgctt cagcccgttg atcgggtcgc cgccgatgcc caccgcgctg ctctggccga
     2701 tgccgagctc ggtcagttgc gcgaccgctt cgtaggtcag cgtgcccgag cgcgacacga
     2761 cgccgacgcg cccggccttg tggatgtggc ccggcatgat gccgatcttg atctcgtcgg
     2821 gcgtgatcag ccccgggcag ttcggcccga gcagcagcgt cttcttgccg ccctgggcct
     2881 ccttgacctt catgcggttg cgcaccatca gcatgtcctt gacggggatg ccctcggtga
     2941 tgcagatcgc caggtcgagg tcggcctcga ccgcttccca gatcgccgcg gccgcg
//
LOCUS       DATMJB010000229         7286 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_501198, whole genome shotgun sequence.
ACCESSION   DATMJB010000229 DATMJB010000000
VERSION     DATMJB010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501198"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1234
                     /locus_tag="VNU48_05075"
     CDS             <1..1234
                     /locus_tag="VNU48_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI10682.1"
                     /translation="SITTETGCCIAGCGPAGAVLALLLARAGIDVVVLEKHTDFLRDF
                     RGDTIHPSTLTVLQDLGLLDAFLELPHQEVRELSGDVYGEAVTIADFTHLPAPRPFMV
                     LIPQWDFLDFIADQARALPNFSLRMGAKALGVIEEGARVVGVNVQTAQGGYAIHADLV
                     VAADGRHTTLRASAGLASEDLGAPIDVLWFRMPRDAARDPSRTGGTIRPGAMLVTLNR
                     GDYWQCAFVIPKGRLDALQAEGIEAFRARVCAVAPFLTAAAATLTAWDDVKLLSVQIS
                     RMPRWYRDGLLCIGDAAHAMSPVGGVGINLAIQDAVAASNLLAEALRDKRLATADLAA
                     VQRRREWPARVTQRAQIAVQNEVLAPVLAGTAAPTSVPLPVRLLQRLPLLRRLPARLV
                     GIGVRPERVLTAPHPRKA"
     gene            1294..1908
                     /gene="thrH"
                     /locus_tag="VNU48_05080"
     CDS             1294..1908
                     /gene="thrH"
                     /locus_tag="VNU48_05080"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoserine
                     phosphatase/homoserine phosphotransferase ThrH"
                     /protein_id="HWI10683.1"
                     /translation="MQMICLDLEGVLVPEIWIEFSKRSGIPELARTTRDEPDYDKLMQ
                     FRIALLTQHRLRLADIQAVIAGMGPMDGAKAFLDDLRSRYQVLILSDTFYEFAQPLMR
                     QLGWPTLLCHKLEVDADGFVQRHVLRQPDQKRHAVNALRGLNFQVIAAGDSYNDTGML
                     GAAHAGFFIHPPESIVKQFPQFPVTRSYAELTSAIDAAAVRLRS"
     gene            complement(1909..2670)
                     /locus_tag="VNU48_05085"
     CDS             complement(1909..2670)
                     /locus_tag="VNU48_05085"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HWI10684.1"
                     /translation="MRYRPRVTDATSSHPTERQRFDRVDALRALAMIWMALYHFGFDL
                     DHFGFIRQNFYTDPVWTVQRSCIVSLFLFCAGVGQAIALDQHQRWPRFWRRWAQVAAC
                     ALLVSLGSWWMFPRSYISFGVLHAIALMLIAMRLSAGAGRWLWPLGLLAIALPQLVQH
                     AWFDTRWTNWVGLVTHKPVTEDYVPLLPWVGVMWWGLAAGQWVLARRRAWVTGTLPAP
                     LAPLAALGRWSLSFYMLHQPVLIGLLMAVVALRGA"
     gene            complement(2649..3053)
                     /locus_tag="VNU48_05090"
     CDS             complement(2649..3053)
                     /locus_tag="VNU48_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10685.1"
                     /translation="MSNLIQPSVLLRRVLWADAIVSAVVGALMALGAAALHGLLGLPE
                     MLLLSAGVALLPYAAYLVWLATRPRVPRLAVWVPIVLNLVWAADCGLVLFGGGVQPTA
                     LGEAFIALQVITVLAFAELEFVGLRACAIVPA"
     gene            3181..3996
                     /locus_tag="VNU48_05095"
     CDS             3181..3996
                     /locus_tag="VNU48_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013590639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI10686.1"
                     /translation="MNPSTTLPVGTQLRAWRQRRHLSQLDLSVDAEVSTRHLSFVETG
                     RAMPSREMVLRLANRLEVPLRERNRLLTAAGYAPMYAERPLSDPALQAARDAVELVLK
                     GHEPYPALAVDRHWNLVSHNRALAPLLQSVSPELLKPPVNVLRLGLHPDGLAPRIVNL
                     GQWRAHLLARLRQQVAVSADATLAALADELRAYPAPGESHADVGEAAVVVPLQLATPA
                     GTLSFISTTTVFGTPVDITLSELALETFFPADAATAALLRATLPRAEPMAPAP"
     gene            complement(3993..4511)
                     /locus_tag="VNU48_05100"
     CDS             complement(3993..4511)
                     /locus_tag="VNU48_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI10687.1"
                     /translation="MRITCRMLALPLAIGLGCAAAPAWSAGGGGGGGGGGGGDPPEAA
                     SADPDYRAGLAAAKAKDWRQVVARMGVVTQRSPKNADAWNELGHAYRQLGDMDNSFKN
                     YARALAIDPRHKGAHEYLGEAYLQVGKLDMAEKELQALDKICFISCEEYRDLKEQVGR
                     YKREHAKTASLI"
     gene            complement(4533..5591)
                     /locus_tag="VNU48_05105"
     CDS             complement(4533..5591)
                     /locus_tag="VNU48_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HWI10688.1"
                     /translation="MLLAACGGGGGSMSGGMGTSYVATSLVSDFSASGNPYNSANVDP
                     NLINAWGVAFNPNGFVWVANAGTSTSTLYDGNGVPQSLIVSIPAGTAGAASPTGIVYN
                     ATSDFVVSQGGTSGASIFIFVGEAGTVSGWSPGVNPTSAVKVFDGAAAGAVYKGLALA
                     SSGAANFLYATDFHNGRVDVFDATFTKVATAGGFVDPTLPAGYAPFGIQAIGGSIYVT
                     FAKQDATAHDDVAGAGLGIVDVFDTTGHFVKRLVDVGGSLNAPWGVALAPANFGPLSN
                     ALLVGNFGDGKINAFNATTGATMGALSNAAGAPIAIDGLWGLAFGNGINAQPTNTLFF
                     SGGPSDETHGVYGRIDLH"
     gene            complement(5748..6476)
                     /locus_tag="VNU48_05110"
     CDS             complement(5748..6476)
                     /locus_tag="VNU48_05110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HWI10689.1"
                     /translation="MRASDVRRAALTRPTGVRHAMCAAWLLAGGAAHAQFGGGIAVET
                     DNRFRGVSLTDGQPDLRLSLSYDHDSGVFAGAAATQVEFMRGRRALQLLGYAGYVARV
                     TPELGAEVGVTSSTFRGDTRYDYSEIFAGLSNERGSLRAYYAPRYFGFDQATAYLEFG
                     ANTLLTPRLRAFGHVGVLRTLGGIEGEGGRRTRADLRVGLGFSAAAAVDVQLAWVTAT
                     RGGPYVVEYGARRSSFVLGAQASF"
     gene            complement(6463..>7286)
                     /locus_tag="VNU48_05115"
     CDS             complement(6463..>7286)
                     /locus_tag="VNU48_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWI10690.1"
                     /translation="QLAALHETATGRQVRVAVIDSAVERTHPDLLRQVELSEDFVAGR
                     AVPAEAHGTAVAGIIAAQADNGIGIAGVAPQARVLALRACWQQAAESTLCSSLSLAQA
                     LQFAITHDAQVINLSLSGPPDQLLARLLEVALGRGIAVVAAFDRDAAGGGFPAAHRGV
                     VAVAVADAASGPAPAAAVIAPGRDVPTTATGARWALVSGSSYAAAHVSGLLALLREAR
                     DQRGATATSVASVAARDLVLLPDGRIDACASLSRVAGLCSCDCTAASAGPTVARQ"
BASE COUNT         1099 a         2674 c         2525 g          988 t
ORIGIN      
        1 gtccatcacg actgagacag gctgctgcat cgcggggtgc ggcccggccg gcgcggtgct
       61 ggcgctgctc ctggcccgag ccggcatcga cgtggtcgtg ctcgagaagc acaccgactt
      121 cctgcgcgac ttccgcggcg acacgatcca cccgtcgacg ctcaccgtgc tgcaggacct
      181 cggcctgctc gacgcattcc tcgagctgcc gcaccaggag gtgcgcgaac tcagcggcga
      241 cgtctacggc gaggccgtga cgatcgccga cttcacccac ctccccgccc cgcggccgtt
      301 catggtgctg atcccgcaat gggacttcct ggacttcatc gccgaccagg cgcgggcgct
      361 gccgaacttc tcgctgcgca tgggcgcgaa ggcgctcggc gtgatcgagg aaggcgcgcg
      421 cgtggtcggc gtgaacgtgc agaccgcgca gggcggctac gcgatccacg ccgatctggt
      481 ggtcgccgcc gacggccgcc acaccacctt gcgcgccagc gccggcctgg cgagcgaaga
      541 cctgggcgcg ccgatcgacg tgctctggtt ccgcatgccg cgcgacgcgg cgcgcgatcc
      601 gtcgcgcacc ggcggcacga tccggcccgg cgcgatgctc gtcaccttga atcgcggcga
      661 ctactggcag tgcgcgttcg tcatcccgaa aggccggctc gacgcgctgc aggcagaagg
      721 catcgaggcg tttcgcgcgc gcgtgtgcgc ggtcgcaccg ttcctcacgg ccgccgcggc
      781 gacgctgacg gcctgggacg acgtcaagct gctcagcgtg cagatcagcc gcatgccgcg
      841 ctggtaccgc gacgggctgc tgtgcatcgg cgatgcggcg catgcgatgt cgccggtcgg
      901 cggcgtgggc atcaacctgg cgatccagga tgcggtggcc gcatcgaacc tgctggccga
      961 ggcgctgcgc gacaagcgcc tcgcgaccgc cgacctcgcc gccgtgcagc gccgccgcga
     1021 gtggccggcg cgcgtgacgc agcgcgcgca gatcgcggtg cagaacgagg tgctggctcc
     1081 ggtgctcgcc ggcaccgcgg cgcccacgag tgtgccgctg ccggtgcggc tgctgcagcg
     1141 cctgccgctg ctgcgccggc tgccggcgcg gctggtgggc atcggcgtgc ggcccgagcg
     1201 cgtgctgacc gcgccgcacc cgcgcaaggc ctgagcccgc cgcgtcgggt acgatccgcg
     1261 ccgccttgcc acgaacgcgc cggagacaac cccatgcaaa tgatctgcct cgacctcgaa
     1321 ggcgtgctcg tgcccgagat ctggatcgag ttctcgaagc gcagcggcat ccccgaactc
     1381 gcgcgcacca cgcgcgacga gcccgactac gacaagctga tgcagttccg catcgcgctg
     1441 ctcacgcagc accggctcag gctcgccgac atccaggccg tgatcgccgg catgggcccg
     1501 atggacggcg cgaaagcctt cctcgacgac ctgcgcagcc gctaccaggt gctgatcctg
     1561 tcggacacgt tctacgagtt cgcgcagccg ctgatgcgcc agctcgggtg gccgacgctg
     1621 ctgtgccaca agctcgaggt cgacgccgac ggcttcgtgc agcgccatgt gctgcgccag
     1681 cccgaccaga agcgccatgc tgtcaacgcg ctgcgcggcc tgaacttcca ggtcatcgcg
     1741 gccggcgatt cgtacaacga caccggcatg ctcggcgcgg cgcacgccgg cttcttcatc
     1801 cacccgcccg agagcatcgt caagcagttc ccgcagttcc cggtgacgcg cagctatgcc
     1861 gagctgacga gcgcgatcga cgctgcggca gtgcgacttc gcagctgatc aggcaccgcg
     1921 cagcgcgacc acggccatca gcagcccgat cagcaccggc tggtgcagca tgtagaagct
     1981 caggctccag cggcccagcg ccgcgagcgg cgcgagtggc gcgggcagcg tgccggtgac
     2041 ccacgcgcgc cgccgcgcca gcacccactg tccggccgcg agaccccacc acatcacacc
     2101 aacccagggc agcagcggca cgtagtcctc ggtgacgggc ttgtgcgtga ccaggccgac
     2161 ccagttggtc cagcgcgtgt cgaaccacgc gtgttgaacg agttgcggca gcgcgatcgc
     2221 gagcagcccg agcggccaca accagcgccc ggcgccggcg ctcagccgca tcgcgatcag
     2281 catcagcgcg atcgcgtgca gcacgccgaa gctgatgtag ctgcgcggga acatccacca
     2341 cgagcccagg ctcaccagca gcgcgcacgc cgccacctgt gcccagcggc gccagaagcg
     2401 cggccaacgc tggtgctggt cgagggcaat cgcctggccg acgccggcgc agaacaggaa
     2461 caggctgacg atgcagctgc gctgcacggt ccagaccggg tcggtgtaga agttctggcg
     2521 gatgaagccg aagtggtcga ggtcgaagcc aaagtggtac agcgccatcc agatcatcgc
     2581 cagcgcgcgc agcgcatcga cgcggtcgaa gcgctggcgc tcggtggggt gtgaagaggt
     2641 cgcgtcggtc acgcggggac gatagcgcat gcgcgcaggc cgacgaactc gagctcggcg
     2701 aacgccagca ccgtgatcac ctgcagcgcg atgaaggcct cgccaagtgc ggtcggctgc
     2761 acgccgccac cgaacagcac gaggccgcag tcggccgccc agacgagatt cagcacgatc
     2821 ggcacccaca ccgcgaggcg cggcacgcgc gggcgtgtcg ccagccagac caggtaggcg
     2881 gcatacggca gcagcgcgac gccggcgctc agcaacagca tttccggcaa gccgagcagc
     2941 ccgtgcaacg ccgcggcgcc gagcgccatc agcgcgccga cgacggcgct gacgatcgcg
     3001 tcggcccaca gcacgcgacg cagcagcacg gagggttgaa tcagattcga catgacaggc
     3061 tccttgtgaa cattgcgctg caaggttgca gcgtggcgtc atcgtcgagc gcctcggtca
     3121 ccggatcgat tacctcgcag gtcatccgcg ccgcgcgggg cctgcgctat cgtgccgccg
     3181 atgaacccgt ccaccaccct gcccgtgggc acgcagctgc gcgcttggcg ccagcgccgc
     3241 cacctgagcc agctcgatct gtcggtcgac gccgaggtct cgacacgcca cctgagcttc
     3301 gtcgagaccg gccgcgcgat gccgagccgc gagatggtgc tgcgcctggc gaaccggctg
     3361 gaggtgccct tgcgcgaacg caaccgcctg ctgacggcag ccggctacgc gccgatgtac
     3421 gccgagcggc cgctgagcga ccccgccctg caggccgcac gcgacgcggt cgaactggtg
     3481 ctgaagggcc acgagccgta cccggcgctc gcggtcgaca ggcactggaa cctggtctcg
     3541 cacaaccgcg cgctggcgcc gctgctgcag agcgtcagcc cggagctgct gaagccgccg
     3601 gtcaacgtgc tgcggctcgg cctgcacccg gacggcctcg cgccgcgcat cgtcaacctc
     3661 ggccagtggc gcgcgcacct gctggcgcgg ctgcgccagc aggtcgccgt cagcgccgac
     3721 gcgaccctgg ccgcgctcgc agacgagctg cgcgcctacc cggcgccggg cgagtcgcac
     3781 gccgacgtgg gcgaggccgc ggtcgtcgtg ccgctgcagc tcgcgacacc ggccggcacg
     3841 ctgtcgttca tcagcacgac caccgtgttc ggcacgccgg tggacatcac gctgtcggag
     3901 ctcgcgctcg aaaccttctt cccggccgac gccgcgaccg cggcgctgct gcgcgcgacc
     3961 ctgcctcgcg cagagccgat ggccccggcg ccttagatca gcgatgcggt cttcgcatgc
     4021 tcgcgcttgt agcgcccgac ctgctccttc aggtcacggt actcctcgca gctgatgaag
     4081 cagatcttgt cgagcgcctg caattccttc tcggccatgt cgagcttgcc gacctgcaga
     4141 taggcctcgc cgaggtactc gtgcgcgccc ttgtggcgcg gatcgatcgc gagcgcgcgc
     4201 gcgtagttct tgaacgagtt gtccatgtcg ccgagctgcc gataggcatg gccgagttcg
     4261 ttccaggcgt cggcattctt cggactgcgc tgtgtcacca cgcccatgcg cgcgacgacc
     4321 tggcgccagt ccttcgcctt cgcggccgcc aggccggccc ggtagtcggg gtcggcgctc
     4381 gcggcttcgg gagggtcccc gccgccgccg ccgccaccac caccgccgcc cccggcactc
     4441 caggccggcg cggcggcgca gcccagaccg atcgccagcg gcagcgcgag cattcgacat
     4501 gtgattctca tgatgatgac ctccagtcag tgtcagtgca gatcgatgcg tccgtagacg
     4561 ccatgggttt cgtcggacgg gccgccgctg aagaacagcg tgttcgtcgg ttgcgcgttg
     4621 atgccgttgc cgaacgcgag gccccacaga ccgtcgatcg cgatcggcgc gccggcggca
     4681 ttgctcagcg cgcccatcgt cgcgccggtg gtcgcattga acgcgttgat cttgccgtcg
     4741 ccgaaattgc cgacgagcag cgcgttgctg agcggcccga agttcgccgg cgccagcgcc
     4801 acgccccagg gcgcgttcag cgacccgccg acatcgacga gccgcttgac gaagtggccc
     4861 gtcgtgtcga acacatcgac gatgcccagc cccgcgccgg cgacgtcgtc gtgcgcggtc
     4921 gcgtcctgct tcgcgaacgt gacgtagatc gagccgccga tcgcctggat gccgaagggc
     4981 gcgtagcccg ccggcagcgt cgggtcgacg aagccgccgg ccgtcgccac cttcgtgaag
     5041 gtcgcgtcga agacgtcgac gcggccgttg tgaaagtcgg tcgcgtacag gaagttcgca
     5101 gcgccgctgc tggccagcgc caggcccttg tagacggcgc ccgccgcggc accgtcgaac
     5161 accttgacgg cgctggtcgg gttcacgccc ggcgaccagc ccgacacggt gccggcctcg
     5221 ccgacgaaga tgaagatgct cgcgccgctc gtgccgccct ggctgacgac gaagtcgctc
     5281 gtggcgttgt agacgatgcc ggtcgggctc gcggcgccgg ccgtgccggc cggtatcgag
     5341 acgatcagcg actgcggcac gccgttgccg tcgtacagcg tcgaggtcga cgtgcccgca
     5401 ttcgcgaccc acacgaagcc gttcgggttg aacgcgacgc cccaggcgtt gatcaggttg
     5461 gggtcgacgt tggcgctgtt gtacgggttg ccgctggcgc tgaaatcgct gacgaggctg
     5521 gtggcgacat agctggtgcc catgccgccg gacatcgacc caccaccgcc gccgcaggca
     5581 gcgagcagca tcacacccag gccgacggcc caccagcgcc gcccgatgca ggacaggttc
     5641 atcacggtgc tcctcgaagt tgacgaggcg aagactgcgg acgcagcccg gcttcgatga
     5701 ccggtgaatg ccgcttgcgc ggccaaaggt tcaatgcgtc gacgcgctca gaacgaggcc
     5761 tgcgcgccga gcacgaagct gctgcggcgg gcgccgtact cgacgacgta gggcccgccg
     5821 cgcgtggccg tgacccacgc gagctgcacg tcgaccgcag ccgcggcgct gaacccgagc
     5881 ccgacgcgca ggtcggcgcg cgtgcgacgc ccgccctcgc cttcgatgcc gcccagcgtc
     5941 ctgagcacgc cgacgtggcc gaacgcacgc agccgcggcg tgagcagcgt gttcgcgccg
     6001 aactcgaggt aggccgtggc ctggtcgaaa ccgaaatacc gcggcgcgta gtacgcgcgc
     6061 aggctcccgc gttcgttcga gaggccggcg aagatttcgc tgtagtcgta gcgtgtgtcg
     6121 cctctgaagg tcgaactcgt cacaccgact tccgcgccga gttcgggggt cacgcgcgcg
     6181 acatagccgg cgtagcccag cagctgcagc gcgcggcggc cgcgcatgaa ctcgacctgg
     6241 gtcgccgccg cgccggcgaa caccccgctg tcgtggtcgt agctcaggct caggcgcaga
     6301 tcaggctggc cgtcggtcag cgagacgccg cgaaaccggt tgtcggtctc gacggcgatg
     6361 ccgccgccga actgggcgtg tgccgcgccg ccggcgagca gccaggccgc gcacatcgcg
     6421 tggcggaccc cggtcgggcg cgtcagcgcg gcgcggcgca cgtcactggc gcgcaaccgt
     6481 cgggccggcg ctcgctgccg tgcagtcgca gctgcaaagg cccgcgacac ggctcagcga
     6541 ggcgcacgcg tcgatccgcc cgtcgggcag cagcaccaga tcgcgcgcgg ccacgctcgc
     6601 gacgctggtt gccgtcgccc cgcgctggtc gcgcgcctcg cgcaacaatg cgagcaggcc
     6661 gctgacatgc gccgccgcgt aggaggagcc cgagaccagc gcccagcgcg ccccggtcgc
     6721 ggtggtcgga acgtcgcgcc cgggcgcgat cacggcggcc gccggcgccg gccccgacgc
     6781 cgcgtcggcg accgcgaccg cgaccacgcc gcggtgcgcg gccggaaacc cgccgccggc
     6841 ggcgtcgcga tcgaacgctg cgacgacggc gatgccgcgc ccgagcgcga cctcgagcag
     6901 ccgcgccagc agctgatcgg gcggcccgct caagctcagg ttgatgacct gcgcatcgtg
     6961 cgtgatcgcg aactgcagcg cctgcgccag gctcaggctg ctgcacagtg tggactcggc
     7021 ggcctgctgc cagcacgcgc gcagcgcgag cacacgcgcc tgcggtgcga caccggcgat
     7081 gccgatgccg ttgtcggcct gcgccgcgat gatgcccgcc accgccgtgc catgggcctc
     7141 ggcgggaacg gcgcggccgg cgacgaagtc ctcgctcagc tcgacctgtc gcagcaggtc
     7201 cggatgcgtg cgttcgaccg cgctgtcgat caccgcgacg cgcacctgac gccccgtcgc
     7261 ggtctcgtgg agggccgcga gctgcc
//
LOCUS       DATMJB010000230         2128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_307746, whole genome shotgun sequence.
ACCESSION   DATMJB010000230 DATMJB010000000
VERSION     DATMJB010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307746"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1793)
                     /locus_tag="VNU48_05120"
     CDS             complement(<1..1793)
                     /locus_tag="VNU48_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI10691.1"
                     /translation="MLSRATAPRRSFIRRGCAAALAALLAPWARAEVPAAAATPKKVL
                     RYAFEVAETGLDPAKISDLYSRTLTPHIFEALYRYDHLARPVKLKSLTADGMPEASAD
                     FRVWTIKVKPGIYFADDPAFKGRKRELVAQDYVYAIKRFADPANKSPAWSSLEADGVV
                     GLAAVRQAALDTRRPFDYDREIEGLRALDRYTLQFKLNEARPRFVNNLAASDLYGAVA
                     REVVEFYGEKIDAHPVGTGPFRLAQWRRSSFIVFERNPGFRAMVYDAEPAADDAEGQA
                     LLARFKGRRLPMVDRVEISIIEEEQPRWLAFVNDEADFAYRVGYQFAPQAMPNGKVAP
                     NLAKRGIRGYPVVEAAANFFFFNMEDPTVGGYSAEKVALRRAIGLGLDVKGIIDYAYS
                     GLGAPAQGATLPKTSAYDPKFKSEFSDYDPARAKALLDLYGYTDRSGDGWRDLPDGTP
                     LVLKVSTTPNQRDRKIAEVLTKNMKALGIRVQSLTAQWPENLKAARAGKLQMWALGLS
                     AASPDAAGSYARFDSHQIGGQNLARVRLPAFDTLYNQLQTLPDGPERLAAFREMDRLS
                     VAYMPYKFSLNRVSLDMAQPQLIGYRRTPFW"
     gene            1955..>2128
                     /locus_tag="VNU48_05125"
     CDS             1955..>2128
                     /locus_tag="VNU48_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI10692.1"
                     /translation="MAAYLIRRLWQMIPTLLGVVLLVFLLFKAFGGDPAEILGGLNAT
                     QQQVDAIRQQLGLN"
BASE COUNT          308 a          712 c          764 g          344 t
ORIGIN      
        1 tgccagaacg gcgtgcggcg gtagccgatg agttgcggct gggccatgtc gagcgagacc
       61 cggttcagcg aaaacttgta gggcatgtag gccaccgaca ggcggtccat ctcgcgaaac
      121 gcggcgagcc gctcggggcc gtcgggcagc gtctggagct ggttgtagag cgtgtcgaat
      181 gcgggcagcc gcacgcgcgc caggttctgg ccgccgatct ggtggctgtc gaagcgcgca
      241 taggaaccgg cggcatccgg gctcgcggcc gacaggccga gcgcccacat ctgcagcttg
      301 ccggcgcgcg cggccttcag gttctcgggc cactgcgccg tcagcgactg aacgcggatg
      361 cccagcgcct tcatgttctt cgtcagcacc tcggcgatct tgcggtcgcg ctggttcggc
      421 gtcgtcgaga ccttcagcac cagcggcgtg ccgtcgggca ggtcgcgcca gccgtcgccg
      481 ctgcggtcgg tgtagccgta caggtcgagc agggccttgg cgcgcgccgg gtcgtagtcg
      541 ctgaactcgc tcttgaactt cggatcgtag gcgctggtct tgggcagggt cgccccttgc
      601 gccggcgcgc ccaggccgct gtaggcgtag tcgatgatgc ccttcacgtc cagccccagg
      661 ccgatcgcgc ggcgcagcgc gaccttctcg gcgctgtagc cgccgacggt cggatcctcc
      721 atgttgaaga agaagaagtt cgccgccgct tcgacgaccg ggtagccgcg gatgccgcgc
      781 ttggccaggt tcggcgcgac cttgccgttg ggcatggcct gcggcgcgaa ctggtagccg
      841 acgcggtagg cgaagtcggc ctcgtcgttg acgaacgcca gccagcgcgg ctgctcctcc
      901 tcgatgatcg agatctcgac ccgatcgacc atcggcaggc gccggccctt gaagcgtgcg
      961 agcagcgcct ggccctcggc atcgtcggcc gcgggctcgg cgtcgtagac catcgcgcgg
     1021 aagcccgggt tgcgctcgaa cacgatgaac gagctgcgcc gccactgcgc gagccggaac
     1081 gggccggtgc ccaccgggtg cgcatcgatc ttctcgccgt agaactcgac cacctcacgc
     1141 gccactgcgc cgtacaggtc gctggccgcg aggttgttga cgaagcgcgg ccgcgcctcg
     1201 ttcagcttga actgcagcgt gtagcgatcg agcgcgcgca ggccctcgat ctcgcggtcg
     1261 tagtcgaagg gtcgccgggt gtcgagcgcc gcctggcgca cggcggccag gcccacgacg
     1321 ccgtccgctt cgaggctgga ccacgccggg ctcttgttgg ccgggtcggc gaagcgcttg
     1381 atcgcataga catagtcctg cgcgacgagc tcgcgcttgc ggcccttgaa cgccgggtca
     1441 tcggcgaagt agatgccggg cttgaccttg atcgtccaga cgcgaaagtc ggccgaggct
     1501 tcgggcatgc cgtcggcggt cagcgacttg agcttcaccg gccgcgcgag atgatcgtag
     1561 cgatacagcg cctcgaagat gtgcggcgtc agcgtgcgcg agtacaggtc gctgatcttg
     1621 gccgggtcga ggccggtctc ggccacttcg aaggcgtagc gcagcacctt cttcggcgtg
     1681 gccgcggcgg ccggcacttc ggcgcgcgcc cacggcgcca gcagggcggc gagcgcggcg
     1741 gcgcagccgc ggcggatgaa cgagcggcgc ggcgcggtgg cgcgagacag catcgaaggc
     1801 gttccttgtg ggatcacggt gaaggccccg ggagcccggc agttgctcgg gcgtagactc
     1861 ccgaccgctc gcattgtcgc gtccgccgcc ccgccacgag cgcggcggcg cgcacgatgc
     1921 cacgatctga ctggatgagg agtgtgtgac agccatggcg gcctacctga tccgacgcct
     1981 gtggcagatg atcccgacac tgctcggcgt cgtgctgctg gtgttcctgt tgttcaaagc
     2041 cttcggcggc gaccccgccg aaatcctcgg cggcctgaac gccacacagc agcaggtcga
     2101 cgcgattcgc cagcagctcg ggctgaac
//
LOCUS       DATMJB010000231         2044 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_161186, whole genome shotgun sequence.
ACCESSION   DATMJB010000231 DATMJB010000000
VERSION     DATMJB010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161186"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VNU48_05130"
     CDS             <1..359
                     /locus_tag="VNU48_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HWI10693.1"
                     /translation="YKHGSRNKAAAIAACDDLQLAGQALCLEAMTGRVVSEGALYYAA
                     SRRRRIVPITAALRTQVSETAGAVRGMLDSGKLPPPTQDVRRCNGCSLRERCQPEALG
                     RLRAADFSRSPFDPDA"
     gene            377..1417
                     /gene="cas1c"
                     /locus_tag="VNU48_05135"
     CDS             377..1417
                     /gene="cas1c"
                     /locus_tag="VNU48_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HWI10694.1"
                     /translation="MQLLNTLYVTLPDSRLRLDNDTLRVEVGSGDDRETRLRVPLHHL
                     QAVVCFGHVGLSALLMHRLADDAIALVLLDANGRFKARLEGAFAGNVLLRRAQHRAVD
                     DAGFTLALARAVVAGKLQNQRQVLQRGARETVQAEDQAKLIRASQDLAASLRALPTAT
                     TLDTLRGIEGEAARNYFSALNLLVRPDLRELFSMDGRSRRPPRDRMNALLSFLYSMWT
                     NDCRSACEAAGLAPQLGFLHAVRPGRAALALDLVEEFRPWADRLALTLINRGQLGADD
                     FDLREGGAVLLQGDARKAVVVAHQERKQDVLTHPLLAEAVPLGMVPLVQARLLARHVR
                     GEAEGYLPFRVK"
     gene            1427..1717
                     /gene="cas2"
                     /locus_tag="VNU48_05140"
     CDS             1427..1717
                     /gene="cas2"
                     /locus_tag="VNU48_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HWI10695.1"
                     /translation="MLVIVCYDVNTETREGRRRLRRVAKVCEGTGQRVQKSVFECQVT
                     LMQLDDLERRLLAEVNLSQDCLRLYRLPEGKGSEVREHGKFKATDFDAPLVL"
BASE COUNT          327 a          698 c          692 g          327 t
ORIGIN      
        1 aatacaagca cggctcacgc aacaaggccg ccgccattgc cgcttgcgac gatctgcagc
       61 tcgccgggca agccttgtgc ctcgaagcca tgacgggtcg cgtcgtcagc gaaggcgccc
      121 tgtactatgc cgcctcccgc cggcggcgaa tcgtgccgat caccgcggcg ctgcgcacgc
      181 aggtgagcga gaccgccggc gcggtacgcg gcatgctcga cagcggcaag ttgccgccgc
      241 ccacgcaaga cgtgcggcgc tgcaacggct gctccttgcg cgagcgctgc cagcccgaag
      301 ccttggggcg gctgcgggcg gccgacttca gccgcagccc gttcgacccc gatgcttgac
      361 ccgtcccgga gcggccatgc aattgctcaa caccttgtat gtcacgctgc ccgacagccg
      421 tctgcgcctg gacaacgaca ccctgcgcgt cgaggtcgga tcgggtgatg atcgagagac
      481 gcgcttgcgc gtgccgctgc accacctgca ggccgtggtg tgcttcggcc atgtcggcct
      541 gagcgcactg ctgatgcacc gcttggccga cgacgcgatc gcgcttgtgc tgctggacgc
      601 caacggccgc ttcaaggcgc ggctcgaagg tgcgttcgcc ggcaacgtgc tgctgcggcg
      661 agcccagcac cgagccgtcg acgacgccgg cttcacgctc gcgcttgccc gcgccgtggt
      721 cgccggcaag ctgcagaacc agcggcaggt gttgcagcgc ggtgcacgcg agaccgtgca
      781 ggccgaggat caggccaagc tgatccgcgc gtcgcaagac ctggccgcaa gcctgcgcgc
      841 gctgcccacc gcgaccacgc tggacacctt gcgcggcatc gagggcgaag ccgcgcgaaa
      901 ctacttctct gcgctcaacc tgctggtgcg gcccgacctg cgcgagctgt tcagcatgga
      961 cggccgcagc cgccggccac cgcgagaccg catgaatgcg ctgctgtcgt ttctgtactc
     1021 gatgtggacc aacgactgcc gctcggcgtg cgaggccgcc ggcctcgccc cgcagctcgg
     1081 tttcttgcat gcggtccgcc ccggccgggc cgcgctggcg ttggacctgg tcgaagagtt
     1141 tcggccctgg gccgaccggt tggcgctgac gctgatcaac cgcgggcagc ttggcgccga
     1201 cgatttcgac ttgcgcgaag gcggcgccgt gctgcttcaa ggcgacgccc gcaaggcagt
     1261 cgtcgtcgcc catcaagagc gaaaacagga cgtgctcacc catccactgc tggccgaggc
     1321 cgtgccgctg gggatggtgc cgctggtgca ggcccgcctg ctcgcccggc acgtgcgcgg
     1381 cgaggctgag ggctacctgc cgttccgcgt gaagtgagca agggccatgc tcgtcatcgt
     1441 ctgctacgac gtcaataccg aaacacgcga agggcgacgc cgactgcggc gcgtcgcgaa
     1501 ggtgtgcgaa ggcaccggcc aacgcgtgca gaaatcggtg ttcgaatgcc aggtgacgct
     1561 gatgcaactg gacgacctcg aacgcaggtt gctggccgag gtgaacctct cgcaagactg
     1621 cttgcgcctg taccggctgc ccgagggcaa aggcagtgaa gtccgcgagc acggcaagtt
     1681 caaggcaaca gacttcgacg cgccgctggt cctatgatgg tcgccgcgaa cccgaagtga
     1741 tcgccccgga cacggtgggt tcgcgcgccg cgcaagccgt tgaatcctcg atgtattttc
     1801 gaaacggtca ccgtgaaacc gcgcccgctg ctccgagcgt gggaaggttc gcgggattag
     1861 ctatgaaagt cgttgcaggg cttcaccttg attgcgtggc gtcgcgcgcg tctcacgacg
     1921 cgcgcgtgga ttgaaaccaa tccggctcgc cgtcggcgtc gaccgcgttc cagtcgcgcg
     1981 cgtctcacga cgcgcgcgtg gattgaaacc gctggtggtt gtagcgcgcg gtcgggcact
     2041 tgcc
//
LOCUS       DATMJB010000232         2597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_1946, whole genome shotgun sequence.
ACCESSION   DATMJB010000232 DATMJB010000000
VERSION     DATMJB010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1946"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..323
                     /gene="rplW"
                     /locus_tag="VNU48_05145"
     CDS             39..323
                     /gene="rplW"
                     /locus_tag="VNU48_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWI10696.1"
                     /translation="MAQVLIAPIVSEKATMAAEKHNQVLFKVMRDATKPEIKAAVELM
                     FKVEVESVSTVIQKGKVKRFGKSIGRRDHVKKAYVSLKKGQELNFSGEGA"
     gene            323..1147
                     /gene="rplB"
                     /locus_tag="VNU48_05150"
     CDS             323..1147
                     /gene="rplB"
                     /locus_tag="VNU48_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWI10697.1"
                     /translation="MPVIKIKPTSPGRRGVVKVVHPHLYKGKPEASLLEPQMQNAGRN
                     NNGHITIRHRGGGHKHHYRVVDFVRNKDAIPAKVERIEYDPNRTAHIALVCYADGERR
                     YVIAPRGLEVGATILSGADAPIKAGNTLPIRNIPVGSIIHAIEMLPGKGAQIARSAGT
                     SATLLAREGVYAQVRLRSGEVRKIHIDCRATIGEVSNEEHQLRQYGKAGAIRWKGIRP
                     TVRGVAMNPVDHPHGGGEGRTGEGQAPVSPWNTLTKGYRTRNNKRTQTMIVARRKK"
     gene            1157..1435
                     /gene="rpsS"
                     /locus_tag="VNU48_05155"
     CDS             1157..1435
                     /gene="rpsS"
                     /locus_tag="VNU48_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWI10698.1"
                     /translation="MTRSLKKGPFVDHHLQAKVEKAVATKDKKPIKTWSRRSTILPDF
                     IGITIAVHNGKQHVPVYISDQMVGHKLGEFALTRTFKGHPADKKANKK"
     gene            1447..1776
                     /gene="rplV"
                     /locus_tag="VNU48_05160"
     CDS             1447..1776
                     /gene="rplV"
                     /locus_tag="VNU48_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWI10699.1"
                     /translation="METRSIVRGVRLSADKGRLVADLVRGKKVDQALNILTFTPKRAA
                     GIIKKCLESAIANAEHNDGADIDELKVKTIYVEQGATLKRFSARAKGRGNRISKPTAH
                     IYITVGN"
     gene            1792..>2597
                     /gene="rpsC"
                     /locus_tag="VNU48_05165"
     CDS             1792..>2597
                     /gene="rpsC"
                     /locus_tag="VNU48_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWI10700.1"
                     /translation="MGQKIHPTGFRLPVTRNWASRWYASNKNFATMLAEDLKVREYLK
                     TKLKSAAVSRILIERPAKNARITIYSARPGVVIGKKGEDIEALKAELTRRLGVPVAVN
                     IEEVRKPEVDAQLIADSITQQLEKRIMFRRAMKRAMQNAMRLGAQGIKLMSSGRLNGI
                     EIARCEWYREGRVPLHTLKADIDYGFSEAKTTYGIIGVKCWVYRGDRLANGEAPGIVT
                     PPGAEDDRRSRRPGGRPGGPGAPGGRGRPGGPGGDRGPRPATASDRPAATA"
BASE COUNT          544 a          852 c          849 g          352 t
ORIGIN      
        1 cagcaaccgt tcaaaagccg aagcacaacg ccgaccgctt ggcccaggtg ttgatcgccc
       61 cgatcgtgtc cgaaaaggca acgatggcgg ccgagaagca caaccaggtg ctgttcaagg
      121 tgatgcgcga cgcgaccaag cccgagatca aagcggctgt tgaactgatg ttcaaggtcg
      181 aagtcgagtc cgtctcgacc gtgatccaaa agggcaaggt caagcgcttc ggcaagtcga
      241 tcgggcgccg cgaccacgtc aagaaggcgt acgtgtcgct caagaagggc caagagctca
      301 acttctccgg ggagggcgcg taatgcccgt catcaagatc aaaccgacct cgccgggccg
      361 tcgtggcgtg gtgaaggtgg tgcacccgca cctgtacaag ggcaagcccg aggcatcgct
      421 gctcgagccc cagatgcaga acgcgggccg caacaacaac ggccacatca cgattcgcca
      481 tcgcggcggt gggcacaagc atcactatcg cgtcgtcgat ttcgtgcgca acaaggacgc
      541 gatcccggcg aaggtcgagc gcatcgagta cgacccgaac cgcaccgcgc acatcgcgct
      601 ggtctgctac gccgatggcg agcgccgcta cgtgatcgcc ccgcgcggtc tcgaggtcgg
      661 tgcgaccatc ctgagcggcg ccgacgcgcc gatcaaggcg ggcaacacgc tgccgatccg
      721 caacatcccg gtgggttcga tcatccacgc gatcgagatg ctgccgggca agggcgcgca
      781 gatcgcgcgc tcggccggca cctcggcgac gctgctggcg cgcgaaggcg tctacgcgca
      841 agttcgcctg cgctcgggcg aagtccgcaa gatccacatc gactgccgcg ccacgatcgg
      901 cgaggtgtcc aacgaagagc atcagctgcg ccagtacggc aaggccggcg cgatccgctg
      961 gaagggcatc cgcccgacgg ttcgcggcgt cgcgatgaac ccggtcgatc acccgcacgg
     1021 tggtggtgaa ggccgcaccg gcgagggcca ggcgcctgtg tcgccgtgga acacgctgac
     1081 caagggctac cgcactcgca acaacaagcg cacgcagacg atgatcgtcg cgcgccgtaa
     1141 gaagtaagga acggccatga ctcgttcact caagaagggc ccgttcgtcg accatcacct
     1201 gcaggcgaag gtcgagaagg cggtggccac gaaagacaag aagccgatca agacctggtc
     1261 gcgccgctcg acgatcctgc ccgatttcat cggcatcacg atcgccgtgc acaacggcaa
     1321 gcagcacgtg ccggtctaca tcagcgacca gatggtcggc cacaagctcg gtgaatttgc
     1381 gctgacgcgc acgttcaagg ggcacccggc cgacaagaag gccaacaaga aataaggatg
     1441 acgacgatgg aaacccgttc aatcgtccgc ggtgtccgcc tgtctgccga caagggccgg
     1501 ctggtggccg acctggtgcg cggcaagaag gtggaccagg cgctcaacat cctgaccttc
     1561 accccgaagc gcgccgccgg catcatcaag aagtgcctcg agtccgcgat cgcgaacgcc
     1621 gagcacaacg acggcgccga catcgacgag ctcaaggtca agacgatcta tgtcgagcaa
     1681 ggtgccacgc tgaagcgttt ctccgcccgg gccaagggcc gcggcaaccg catcagcaag
     1741 ccgaccgcgc acatctacat caccgtcggc aactgaggct caaggaacac catgggacaa
     1801 aagattcacc cgaccgggtt ccggctgccg gtcacgcgca actgggcgtc gcgctggtac
     1861 gcgtcgaaca agaacttcgc gaccatgctg gcggaagacc tgaaggtgcg cgagtacctg
     1921 aagaccaagc tgaagagcgc cgcagtctcg cgcatcctga tcgagcgccc cgccaagaac
     1981 gcgcgcatca ccatctactc ggcacgcccg ggtgtggtga tcggcaagaa gggcgaggac
     2041 atcgaagcgc tgaaggccga actgacgcgc cgtctcggcg tgccggtggc ggtgaacatc
     2101 gaagaagtgc gcaagcccga ggtcgatgcc cagctgatcg ccgacagcat cacgcagcag
     2161 ctcgagaagc gcatcatgtt ccgccgcgcg atgaagcgcg cgatgcagaa cgcgatgcgc
     2221 ctgggcgccc agggcatcaa gctgatgtcg tcgggccgcc tgaacggcat cgagatcgcg
     2281 cgttgcgagt ggtaccgcga aggccgcgtt ccgcttcaca ccctgaaggc cgacatcgac
     2341 tacggcttct ccgaagccaa gacgacctac ggcatcatcg gcgtgaagtg ctgggtctac
     2401 cgcggtgacc ggctggccaa cggcgaagcg ccggggatcg tcacgccgcc gggtgccgag
     2461 gacgatcggc gctcgcgccg tccgggcgga cgcccgggtg gtccgggtgc accgggcggc
     2521 cggggccgtc cgggtggtcc cggcggggac cgcggtcccc gtccggcgac ggcttccgat
     2581 cgtccggctg cgactgc
//
LOCUS       DATMJB010000233         3279 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_669501, whole genome shotgun sequence.
ACCESSION   DATMJB010000233 DATMJB010000000
VERSION     DATMJB010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669501"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1860
                     /locus_tag="VNU48_05170"
     CDS             <1..1860
                     /locus_tag="VNU48_05170"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI10701.1"
                     /translation="GLPQEVLERIVGAAEGNPLFVEQMLSMLIDNRALRQQDGRWVRG
                     ERYAEIAVPPTIQALLEARLDRLAREERVTVEPASVIGMQFALPAVTALAPEAARAHV
                     GERLATLAHKQFIQPAESSEGDVLYRFHHHLVRETVYGGLLKRSRATLHIDFVRWADR
                     VNAERGRALEFEEILGYHLEQAHRYLRELGPLDEQGLAIGLDAARRLTAAARRAFARG
                     DMHAAANLYRRAVALLPGTDANRLALLPELGETLMELGDFDDARQVLDEAAASAEQSG
                     NQRIRASARIVRMLVRLYSAETGDWSSAALQVAEETIPLLEREAAHDELANAWRLSSQ
                     VHGIAARFGQMVDAINKAIGHARLAGDARMVARAGMALATTALYGSTPVAQAIEQCDR
                     IVADGLTDRQVEGIVICTLAHLLAMNAEFDKARELYRRGRALLRDLGQGVNAASTGLD
                     VARVEWLAGDLVAAERELRGDLDFLAQRGETFFLPTMAAQLSRIVRDLGDADEALALS
                     KTAEEAATEDDTASQVLWRVARATLVARHGDLAEAEALARRAVELSGGTDAPIFQADA
                     LVELASVLSLGGRVDEARTTLGAALGLYTDKGDIVSAARAAALADRLEPSTPS"
     gene            complement(1889..3106)
                     /locus_tag="VNU48_05175"
     CDS             complement(1889..3106)
                     /locus_tag="VNU48_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10702.1"
                     /translation="MTDSTFADGAPGARGGIVSRAAGWMGSALLAAGLLAGTDAVLAA
                     NANFAVTVTPVAASASSPAAVSVGRNGMTTYAAYQVSITNSSGNTSNAIRFSASTDVG
                     GDLTANGGNAGALAPYVETDPTGIPPTACQPTATGVQCNFGQMKNGDKESFVLIFAAP
                     KLGDADHWAVDANITLSWALDYSSGGSSGSASSAYCTIGVTTTPINPCVGSAPTKLVT
                     TLTDDILSSFVTYIPSFGGTFFTGNGASVLPPAGTDLLPTAAIKVTVPTGQNLTTAQA
                     DLTVVLGSATSATTTTNTAVIQIPNNNQLFGSYATIELRRDASTIASGAKIANAAVLY
                     THDDTCAPTPSTCTFNPLPPCPVGGVPTSSAPVCEMLSQRTEFTKKNAPTPADVGDWL
                     FVVHALENGVSRF"
BASE COUNT          527 a         1081 c         1160 g          511 t
ORIGIN      
        1 ggcctgcctc aagaagtgct cgagcggatc gtcggcgcgg ccgaaggcaa cccgttgttc
       61 gtcgagcaga tgctgtcgat gctgatcgac aaccgcgcgc tgcgccagca ggacgggcgc
      121 tgggtgcgcg gcgaacgcta cgccgagatc gcagtgccgc cgaccatcca ggcgctgctc
      181 gaagcccggc tggaccggct tgcgcgggag gagcgggtga cggtcgagcc ggcatccgtc
      241 atcggcatgc agttcgcgct gccggccgtg accgcgctgg cgcccgaggc ggcgcgcgcc
      301 catgtcggcg aacgcctggc gacgctggcg cacaagcagt tcatccagcc ggccgagtca
      361 agcgaaggcg acgtgctcta ccgcttccac catcacctgg tgcgcgagac ggtctatggc
      421 ggcctgctca agcgctcgcg ggccaccctg cacatcgact tcgtgcgctg ggcggatcgc
      481 gtcaacgccg agcgcggtcg cgcgctcgag ttcgaggaga tcctcggcta ccacctcgag
      541 caggcgcacc gctacctgcg cgagctcggg ccgctcgacg agcaggggct cgcgatcggc
      601 ctcgacgcgg cgcggcggct gacggccgcg gcgcggcgtg cattcgcccg cggcgacatg
      661 catgccgcgg cgaacctcta ccgccgcgcc gtcgcgctgc tgccgggcac cgacgccaac
      721 cggctcgcgc tgctgcccga actcggcgaa accttgatgg agctcggtga cttcgacgat
      781 gcacgccagg tgctcgacga ggcggcggcc agcgccgagc agagcggcaa tcagcgcatc
      841 cgggcgtcgg cgcggatcgt gcgcatgctg gtgcggctgt acagcgccga gacgggggac
      901 tggagctcgg cggcgctgca ggtggccgag gaaacgattc cattgctgga gcgcgaagcc
      961 gcgcacgacg aactcgcgaa cgcctggcgg ctcagcagcc aggttcatgg catcgccgcg
     1021 cgcttcgggc agatggtcga cgcgatcaac aaggcgatcg gccatgctcg cctcgccggc
     1081 gatgcccgga tggtcgcgcg cgccgggatg gcgttggcga ccacggcgct gtatgggtcg
     1141 acgccggttg cgcaggccat cgagcaatgc gaccggatcg tcgccgatgg cctgaccgat
     1201 cggcaggtgg aaggcatcgt catctgcacc ttggcccacc ttcttgcgat gaacgccgag
     1261 ttcgacaagg cgcgcgagct gtatcgccgt gggcgcgcgc tgctgcgaga cctgggtcag
     1321 ggcgtcaacg cggcatcgac cggactggat gtcgcacgtg tcgagtggct ggcgggcgac
     1381 ctggtcgccg cagaacgcga gctgcgaggc gacctggact ttctcgcgca acgcggcgaa
     1441 accttctttc tgccaacgat ggcggcgcag ctctcgcgca tcgtgcgcga cctgggcgat
     1501 gccgacgaag cactggcgct ctcgaagacc gccgaagagg ccgccaccga agacgacact
     1561 gcctcgcagg tgctctggcg cgtggcgcgc gcgacgctcg tggcgcgcca cggcgacttg
     1621 gccgaagccg aggcgctggc acgccgggcg gtcgagttgt ctggcggcac cgacgcgccg
     1681 atcttccagg ccgacgcgct cgtcgaactc gcctcggtgc tgagcctggg cgggcgggtc
     1741 gacgaagcgc gcacgacgct aggcgcggcg cttgggctct ataccgacaa gggcgacatc
     1801 gtctcggccg cacgcgccgc cgcgctggcc gaccgcctcg agccgtcgac accgagctga
     1861 ccggatcccg ccggcgcgac gcgacgcatc aaaagcgcga gacgccattc tccagcgcat
     1921 ggacgacgaa cagccagtcg ccgacgtcgg cgggcgtggg tgcgttcttc ttcgtgaact
     1981 cggtgcgctg ggacaacatc tcgcacaccg gggccgaact ggtcgggaca ccgccgaccg
     2041 gacaaggggg caatgggttg aacgtgcaag tgctgggtgt gggcgcgcac gtatcgtcgt
     2101 gtgtgtacaa gaccgcggca ttggcgatct tcgcgccgct tgcgatcgtg ctggcgtcgc
     2161 ggcgcagctc gatggtcgcg tagctgccga acagctggtt gttgttcggt atctggatga
     2221 cggccgtgtt cgtggtcgtc gtcgcgctcg tcgcactgcc gagcaccacg gtcaggtccg
     2281 cctgcgcggt ggtcaggttc tgccccgtgg gcacggtgac cttgatcgcc gcggtcggca
     2341 gcaggtccgt gccagcgggc ggcaacaccg acgcgccgtt gccggtgaag aaggtgcccc
     2401 cgaagctcgg gatgtacgtg acgaagctcg aaaggatgtc gtcggtgaga gtcgtgacga
     2461 gcttggtcgg cgcagacccg acgcaggggt tgatcggggt ggtggtgacg ccgatcgtgc
     2521 aataggccga cgacgctgac ccggaactac cgccgctcga gtagtcgagg gcccagctca
     2581 aggtgatgtt cgcgtcgaca gcccaatgat cggcgtcgcc gagcttcggg gccgcgaaga
     2641 tcaggacgaa cgattccttg tccccattct tcatctgccc aaagttgcac tggacgcctg
     2701 tcgcagtggg ttggcacgcc gtcggcggga tacccgtcgg gtcggtttcg acatacggtg
     2761 cgagcgcgcc cgcgttgcct ccgttcgcgg tcaagtcacc gccgacatcg gtcgatgcgc
     2821 tgaagcggat cgcgttgctc gtgttgcccg agctgttggt aatcgatact tgataggcgg
     2881 cgtaggtcgt catgccattg cgaccgaccg agaccgcggc cgggctgctg gcactcgcag
     2941 cgaccggcgt cacggtcacg gcgaagttcg cgttcgcagc cagcaccgca tcggtaccgg
     3001 caagcaggcc ggcagccaag agcgccgaac ccatccaccc ggcggcgcgc gaaacgatgc
     3061 cgccgcgcgc gcccggcgcg ccgtcggcaa atgtcgaatc agtcattgcg atctcccagt
     3121 tgttcaattg gcgctagcaa acgaccacaa cagccatccc cgaaagggtg gctgtgtcgg
     3181 ccgcacgtct tgcgcgggtg acggtataca gacaagaatt ccttcttgcg accggttaca
     3241 tgttcggtgc caaaccgacg gaagtcaatc cccacaagt
//
LOCUS       DATMJB010000234         5323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_841060, whole genome shotgun sequence.
ACCESSION   DATMJB010000234 DATMJB010000000
VERSION     DATMJB010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841060"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /locus_tag="VNU48_05180"
     CDS             complement(<1..1487)
                     /locus_tag="VNU48_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HWI10703.1"
                     /translation="MPDTLDLVEPPVEAGALRSLASLDERLNPAQGSAFLTGAQALIV
                     VLLMQRERDRLAGMTTGGYVSGYRGSPLGTFDQALWAAAKRLKAAGIVFDPGVNEDLA
                     ATAVWGTQQLDALGPANVDGVFAMWYGKGPGVDRSGDPFKHANYTGTHPRGGVLAVLG
                     DDHPGKSSTVAHQSEQAMAANSIPVLYPSNVEDIVRFGLLGFAMSRYAGCWASLKVVN
                     ETIEQTATVDIDPQAQHIALPDAADLLPPEGVHYRGAFTPARDETILMHHRLPLVLRF
                     ARANRIDQCTMGDESARFGLVSAGKAYQDTRQALSYLGIDDARARTLGLALYKVGMVW
                     PLEPLGLKEFAAGKAELFFIEEKAAFVERQAAAVLYNEAVRPRIVGKYNEHGAALLPL
                     EKQLEPLDLALAIAARLRAAGLADEALEARMALLAPRRGGVIALAAAQDGRPPYFCSG
                     CPHNTSTNVPEGSLALAGIGCHTMAIFHKPRTLLSVQMGGEGMNW"
     gene            complement(1493..2455)
                     /locus_tag="VNU48_05185"
     CDS             complement(1493..2455)
                     /locus_tag="VNU48_05185"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HWI10704.1"
                     /translation="MQNESSMRILVLGAGATGGYYGARLQQAGAQVRFLVRERRRGQL
                     RERGLVVRSALGDMSLPAASLSRDELAGAAAQADVVLLTCKAFELDEAMDTIAPAVAA
                     GALVLPVINGMEAYTRLDKRFGRDKVLGGIAYIATMLMPDGAIQHFGPNDVFVVGARA
                     ASQEPVAQRFHELIARTPGMRRLSADIEQELWEKWVMLASGATATTLMRGSVSEILRA
                     AGGEAVIRQAIDEVTKVASMSGHPPGPASVVMVERVLLDPASPWAASMARDITAGIPR
                     LESQAILGDLIAQAKLVGAAVPLLQTAYCALQVYEAQRAAASAK"
     gene            complement(2533..3726)
                     /locus_tag="VNU48_05190"
     CDS             complement(2533..3726)
                     /locus_tag="VNU48_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWI10705.1"
                     /translation="MQRAYRLHDRAEPAARRRRLHRHLGLNAMVAVHSPLTIQSVDVR
                     AVMVLLKRTPVLRHIGLPPRWPIILIDLHMQEGVVGRSYLQPYLPNGVRYVAAVIHDL
                     VERFKGKPANPVDLFRQMRQSLSPLGLEGVTLAAVSGLDMAIWDALAKAAQMPLARYL
                     GGSVGPVKAYNSGGLWLIDPADVARETEELIAEGQFSAVKLRLGRDSLAQDVASIAAA
                     RRAGGASLQLMADFSQVFTMDEAIARCRTLDDEGLYWIEEPIAYDNFSGLAQLRRRLA
                     TPIQVGENFWGPRDLQIALELDATDFVMPDLMRIGGVTGWMRAAGIAAAKGVPVSTHL
                     YPEFSAHLMRVTETAHWLEWCDWADAILAEPFEVRNGEIHIPDRLGSGIEWNEDAVAK
                     YAFRI"
     gene            complement(3648..4433)
                     /locus_tag="VNU48_05195"
                     /pseudo
     CDS             complement(3648..4433)
                     /locus_tag="VNU48_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248883.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(4711..>5323)
                     /locus_tag="VNU48_05200"
     CDS             complement(4711..>5323)
                     /locus_tag="VNU48_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWI10706.1"
                     /translation="KAFMTRYPHIQVELEEQQSIDVIRQLQTGQADIGIFDWHQPMPG
                     LVVSAYHHDSLVLVMPIGHPLRTRKDIAFVETLDQDHVCLPQGTAARTMMELTASGAG
                     RAMRVRMQVKGFDGICRMAAAGVGLGIVPTSAAADHIRLGRVHAVPLKDEWAARTLKV
                     ALPSQRCPTGGTTELLNHLKSCAAELALQLGRRGRRSAKQAST"
BASE COUNT          879 a         1801 c         1747 g          896 t
ORIGIN      
        1 ctccagttca tgccttcgcc gcccatctgt acggacagca gggtgcgcgg cttgtgaaag
       61 atcgccatgg tatgacagcc gatgcccgcc aatgccaagc tgccttcggg cacgttggtg
      121 gatgtgttgt gcgggcagcc cgagcagaaa tagggagggc ggccgtcttg ggccgccgcc
      181 aatgcaatca cgccgccgcg gcggggggcc agcagcgcca tgcgcgcctc cagcgcctca
      241 tcggccaggc ccgccgcacg aagccgggcg gcgatcgcca gcgccaggtc caggggttcg
      301 agttgcttct cgaggggcag cagcgcagcg ccgtgctcgt tgtatttgcc gacgatgcgc
      361 gggcgcacgg cctcgttata cagcaccgca gcggcttgcc gctcgacgaa agcggccttt
      421 tcctcaatga agaacagctc ggccttgccg gcggcaaatt ccttgagccc gagcggctcc
      481 agcggccaca ccatgccgac cttgtacagc gccaagccga gcgtgcgagc ccgcgcgtcg
      541 tcgatgccca aataggacag cgcctggcgc gtgtcctggt aggccttgcc ggctgacacc
      601 agcccgaacc gcgcgctctc gtcgcccatc gtgcattggt cgatgcggtt ggcgcgcgca
      661 aagcgcagca ccagcggcaa gcggtgatgc atcaggatgg tttcgtcgcg cgccggcgtg
      721 aacgcgccac ggtaatgcac gccctccggc ggcaacagat cggccgcgtc aggcagcgcg
      781 atgtgctgcg cctgcgggtc gatgtcaacc gtggcagtct gctcgatggt ctcgttgacc
      841 accttgaggc tggcccagca gccggcgtag cgcgacatcg cgaagcccag caaaccgaag
      901 cgcacgatgt cctcgacgtt ggacggatac agcaccggga tcgagttggc cgccatcgcc
      961 tgctcgctct gatgcgcaac cgtcgatgat ttgccggggt gatcgtcgcc gagaaccgcc
     1021 aacacaccgc cgcgcggatg cgtgccggtg taattggcgt gcttgaaggg gtctcccgag
     1081 cgatcgacgc cgggcccctt gccgtaccac atcgcgaaca caccatcgac gttggcaggc
     1141 cccagcgcat ccagctgctg cgtcccccac accgctgtcg cggcaaggtc ttcgttgacg
     1201 cccggatcga aaacaatgcc cgccgccttc aggcgcttcg ccgccgccca gagggcctgg
     1261 tcgaaggtgc ccagcggcga accgcgatag ccgctgacat aaccgccggt ggtcatgccc
     1321 gcgaggcggt cgcgttcgcg ctgcatcagc agcacgacga tcaacgcctg cgcgccggtc
     1381 aggaatgccg agccctgcgc cggattcaag cgctcgtcca ggctggcgag cgatcgcagc
     1441 gcgccggctt cgaccggggg ttcgacgaga tcgagggtgt cgggcatggg gttcattttg
     1501 cggacgctgc ggcgcgttgg gcctcgtaga cctgcagagc gcagtaggcg gtctgcagca
     1561 gcggcaccgc tgcgcccacg agcttggctt gcgcaatcag gtcacccagg atggcctgtg
     1621 actccaggcg gggtatgccc gccgtgatgt cgcgtgccat cgacgccgcc cagggcgagg
     1681 ccgggtccag caggacgcgt tcgaccatga cgaccgacgc cgggcccggc gggtggccgg
     1741 acatgctcgc gaccttcgtc acttcatcga tggcctgccg gatcacggcc tccccgcctg
     1801 cggcgcgcag aatctcgctg acgctcccgc gcatcagggt ggtcgccgtg gcgccggacg
     1861 cgagcatcac ccacttctcc cacaactcct gctcgatgtc ggcagacaac ctgcgcatgc
     1921 cgggcgtgcg cgcgatcagt tcgtgaaagc gctgtgcaac gggctcctgc gacgcagctc
     1981 gcgcgccgac gacaaacacg tcgttgggcc cgaagtgctg gatcgcgccg tcgggcatca
     2041 gcatggtggc gatgtaagca atgccgccga gcaccttgtc gcggccgaag cgcttgtcca
     2101 gccgcgtata ggcttccatg ccgttgatga ccggcagcac cagcgcaccc gctgccaccg
     2161 cgggggcgat cgtgtccatg gcctcgtcga gttcgaatgc cttgcaggtc aacagcacca
     2221 catcggcctg cgccgcagcc ccggccagtt catcgcggct gagcgatgct gcgggcaggc
     2281 tcatgtcgcc caaggcgctg cgcacgacca gcccccgctc acgcagttgc ccacggcgcc
     2341 gctcgcgcac aaggaaacgc acctgcgcgc cggcctgttg caggcgcgcg ccgtagtagc
     2401 cgcctgtggc accggcgccc agcaccagga tccgcatgga tgattcgttt tgcatgagct
     2461 ctagtccttc ttctcgaggt tgcaggttga acgcttgatg ctgtgcatga agatgtctcc
     2521 gttccagttt ggtcagatgc ggaaggcgta cttggccacc gcgtcctcat tccattcgat
     2581 tccactgcca agccgatccg ggatgtggat ctcgccgttg cggacctcga aaggctccgc
     2641 caggatcgca tcggcccaat cgcaccactc cagccagtgc gcggtttccg tcacccgcat
     2701 caggtgggcc gagaactcgg ggtacaggtg ggtggacacg ggcacgccct tggccgctgc
     2761 gatccccgcg gcgcgcatcc agccggtgac gccgccgatg cgcatcaggt cgggcatcac
     2821 gaagtccgtg gcgtcgagtt cgagtgcgat ctgcaggtcg cgcgggcccc agaagttctc
     2881 gccgacctgg atcggcgtcg ccagccggcg gcgcagctgc gccagccccg agaagttgtc
     2941 gtacgcgatc ggctcctcga tccagtagag cccttcgtcg tccagcgtac ggcagcgcgc
     3001 aatggcttcg tccattgtga agacctggct gaaatcggcc atgagttgca gcgatgcgcc
     3061 gccggcgcgc cgggcggcgg cgatcgacgc gacatcctgc gccaggctgt cgcgtcccag
     3121 ccgcagcttc acggcgctga actggccctc ggcgatgagc tcctccgtct cgcgcgccac
     3181 gtccgcagga tcgatcagcc acaggccgcc gctgttgtac gccttgaccg ggccgacgct
     3241 gccgcccagg tagcgggcga gcggcatctg tgccgccttg gccagcgcgt cccagatcgc
     3301 catgtcgagg ccggagacgg ccgccagcgt gacgccttcc agccccaggg gcgacaagct
     3361 ctggcgcatc tggcggaaca ggtcgaccgg gttggcgggc ttgcccttga agcgttcgac
     3421 caggtcgtgg atgaccgcgg cgacgtagcg cacgccgttg ggcaggtagg gttgcagata
     3481 gctgcgtccc accacgcctt cctgcatgtg cagatcgatc aggatgatcg gccagcgcgg
     3541 cggcagcccg atgtgcctga gcaccggcgt gcgcttgagc agcaccatca cggcacgcac
     3601 atcgacagac tggatggtga ggggggaatg cacggcaacc atcgcgttca gcccaaggtg
     3661 ccggtgaagg cgccgccgtc gagcagcagg ttctgcccgg tcatgaagcc ggtatgcgcg
     3721 ctgcacagaa aagcgcaggc caggccgaat tcctcgggcg tgccgaagcg cccggccggc
     3781 acctgctgca gcatggcgtc gcgtacttcg tccgcgctct tgccgctgcg cttgcccaac
     3841 atgggcaagc ccaggagcag gcggtcggtc gcgtgcaagc cgggcagcag gccgttgatg
     3901 gtcacgttgt gcttcacgat ctgccgtgac agtccggcta caaagccggt cagccccaag
     3961 cgcgcaccat tggaaagccc gagaaaatca atgggcgact tgacggcgct cgaggtgatg
     4021 ttgacgatgc ggccgaactt gcgctggatc attccatcca cggtgcggcg gatcaactcg
     4081 atgggggcca gcatgttggc gtccagggcg gcgatccagg catcacggtc ccactcgcga
     4141 aagtcgccgg gcggaggccc ccccgcattg gtcaccagaa tgtccggatc gggcatggcc
     4201 gccagaaccg cgtcccggcc tgaggccgtg gcaatgtcgg cagcgaccac gcgcacgttc
     4261 gcgccggtgg cacttctgat ctcgttcgcg gcggcctgca gttgatcgat gccgcgcgcg
     4321 gtaatcaaaa tctcggcgcc ctcgtgggcc agtgccatgg cgcagccctt gccgagaccc
     4381 ttgctggcgc cgcacacgat ggcgcgcctg cccttgatgc tcatgtccat tgttttttct
     4441 gttaagtccg tgttcgtacc gagcttgctg cggttggccc tgcgcgcaac tcatgaactt
     4501 gcaagtggca acaggcagta cgcagcaacg gaacaggccg aaccgggaag ctgacgtcgt
     4561 cgctcgcctg gatcagccgc ccaccgcggc agccacccat cgctccggcg cccggcgccg
     4621 gaatctgcat gccccctgtc gcccgaaacg cgccaacttt atcggcaagg agcggtgcag
     4681 accatggaca tttatggaac ttgatttcga ctaagtcgac gcctgcttgg cagaccgacg
     4741 accgcgccgc ccgagctgga gcgcgagttc tgcggcgcaa cttttcagat ggttgagcaa
     4801 ctccgtcgtg ccgcctgtcg gacagcgctg cgacggcagc gcgactttca aggtccttgc
     4861 ggcccattca tctttcagcg gtactgcgtg cacgcgtcca agcctgatgt gatctgctgc
     4921 agcgctggtg ggcacaatgc ccaggcctac gccggcggcg gccatgcggc agatcccgtc
     4981 gaagcctttg acctgcatgc gcacacgcat cgcgcgtccc gctccagatg cagtcagctc
     5041 catcatcgtg cgggccgctg tgccctgggg caggcagacg tggtcctggt ccagcgtttc
     5101 gacgaaggcg atgtctttgc gcgtgcgcag gggatgccca atcggcatga cgagcaccag
     5161 cgagtcgtgg tgataggcgg acacgaccag ccccggcatc ggctggtgcc agtcgaagat
     5221 gccaatgtcg gcctgtccgg tctgcaactg ccgaatcacg tcgatgctct gctgctcttc
     5281 aagctcgacc tggatatgcg ggtagcgggt catgaaagcc ttc
//
LOCUS       DATMJB010000235         3691 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_744126, whole genome shotgun sequence.
ACCESSION   DATMJB010000235 DATMJB010000000
VERSION     DATMJB010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744126"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VNU48_05205"
     CDS             complement(<1..659)
                     /locus_tag="VNU48_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI10707.1"
                     /translation="MEKTWLKQYPAGVPAEIDVDQYSSLVALLDESFKKYAGRMAYKF
                     MGKSISFAQVDDAARALAAYLQAQGLEKGDRVALMMPNVPQYPVAVAAVLRAGYVVVN
                     VNPLYTPRELEHQLKDSGARLIIMVENFATTLQQVLAAVPTKKIITTALGDMLGFPKS
                     MIVNYMVRKVKKMVPAYDLPGAVSFNDAIVQGRTKTFKPASVGPDDIAVLQYTGGTTG
                     RS"
     gene            complement(784..2205)
                     /locus_tag="VNU48_05210"
     CDS             complement(784..2205)
                     /locus_tag="VNU48_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005666513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWI10708.1"
                     /translation="MQRRPTLRTRPWLLPIATAAALSACAVGPDYERPSAAAPAAFKE
                     APPGAPGWFPAAPADTLERGPWWELFGDAQLNRLAAQVEVSNQNVAAAVAAYAQARAL
                     VAQQRATLFPTIGLNASATRSGGGAALDTGNRLQLGANASWEPDIWGALRRGVEGAQA
                     SAQASAADLAAARLSAQGELATDYFALRAADAQIALTRSSVEGYERSLKITQDRYAAA
                     IAAKTDVLQAQTLLDSTRANLATLAITRAQLEHAIAVLVGKAPAEFALPAADWNTVVP
                     AVPLGVPSQLLQRRPDIASAERAVAAANAAIGVQRSAYFPALTLSGSYGNAGSRVADL
                     IGASTSLWSLGLAATQMLFDAGATHARVEGAEAARDAAVARYRQTVLAAFQAVEDQLA
                     AIRYLAEQADLRRSASQAADLTEQQLLNRYKQGLVAYTDVVTAQVSALNARSTLSQLA
                     SSRQAAAIGLIQALGGGWHAPAP"
     gene            complement(2211..>3691)
                     /locus_tag="VNU48_05215"
     CDS             complement(2211..>3691)
                     /locus_tag="VNU48_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWI10709.1"
                     /translation="FTGGGRAGGGFMFVNLKPLGTKPGQRTDKGSAVIARLRPQLQRV
                     TGLQVFLNTVQDVRSGGRSSNSTYQYTLKSDNVADLRNWTMKLNDEMKRQPELTDVDT
                     DQQENGVQTTVQVDRDAAKRLGVSSSAIDAALYNAFGQRQVATIYTELNQYHVVMEWA
                     PGYTTSPNALADIHVPATRIVESGGQAVAVESTAANAATSSRASTTVVPVSANPGLKS
                     ASTGNVLANTTTSLVPLSTMARFVEEPTATSVNHQDTELATTISFNLADAVTLDQARD
                     AIKTAEANIGMPTTVRGDFAGSALSAQQSNSQQTLLIVAAIVVIYIVLGILYESLVHP
                     VTVLSTLPSAGVGAVLALLLFKMEFSIMALIGVFLLIGIVKKNAILIIDFALEAERSR
                     GLSSLEAVREACLLRFRPILMTTLAAALGALPLAIGFGEGSELRQPLGIAIIGGLIAS
                     QVLTLLTTPVVYLLLDRLRRRGADERHLSRAGNDPPALAGST"
BASE COUNT          504 a         1300 c         1310 g          577 t
ORIGIN      
        1 gatctgccgg tggtgccgcc ggtgtactgc agcaccgcga tgtcgtccgg cccgacactg
       61 gccggcttga aggtcttggt gcggccctgc acgatcgcgt cgttgaagct cacggcaccc
      121 ggcaggtcgt acgccggcac catcttcttc accttgcgca ccatgtagtt gacgatcatc
      181 gacttcggga agccgagcat gtcgcccagc gccgtcgtga tgatcttctt ggtcggcacc
      241 gcggccagca cctgctgcaa ggtggtcgcg aagttctcga ccatgatgat cagccgggcg
      301 cccgagtcct tcagctggtg ctcgagctcg cgcggcgtgt acagcgggtt gacgttgacg
      361 accacgtagc cggcgcgcag caccgccgcg accgcgaccg gatactgcgg cacgttgggc
      421 atcatcagcg cgacgcggtc gcccttctcc aggccctgcg cctgcaggta cgcggccagc
      481 gcgcgcgcgg catcgtcgac ctgcgcgaag ctgatggact tgcccatgaa cttgtacgcc
      541 atgcggccgg cgtacttctt gaagctctcg tccagcagcg ccaccagcga gctgtattga
      601 tcgacatcga tctcggccgg cacgccggcg gggtactgct tcaaccaggt cttttccatc
      661 gagaactccg agaaacagcg agggattctg accgagcatg ctcgcccccg gcctaggtat
      721 tgcccccggc cggtcgcgat ggacgcgtgc gcgatgaagg cggcgctggg aatccgagcc
      781 gcgctacggc gccggcgcgt gccagccgcc gcccagcgcc tggatcagcc cgatcgccgc
      841 ggcctgccgg ctcgacgcaa gctgcgacag cgtgctgcgc gcgttcagcg ccgagacctg
      901 cgcggtgacc acgtcggtgt acgccaccag cccctgcttg tagcggttca gcagctgctg
      961 ctcggtgagg tcggcggcct gcgacgcgga gcgccgcaga tcggcctgtt cggccaggta
     1021 gcggatcgcg gcgagctggt cctcgaccgc ctggaacgcc gccagcacgg tctgccggta
     1081 gcgcgccacg gccgcgtcgc gcgcggcctc ggcgccttcg acgcgcgcgt gcgtcgcgcc
     1141 ggcgtcgaac agcatctgcg tggccgcgag cccgagcgac cacaggctgg tcgatgcacc
     1201 gatcagatcg gcgacccggc tgccggcatt gccgtacgag ccgctcaagg tcagcgccgg
     1261 aaagtaggcc gagcgctgca caccgatggc cgcgttcgcc gccgcgacgg cgcgctcggc
     1321 cgacgcgatg tcgggccggc gctgcagcaa ctgcgagggc acgccgagcg gcaccgcggg
     1381 cacgacggtg ttccagtcgg ccgccggcag cgcgaactcg gccggcgcct tgccgacgag
     1441 caccgcgatc gcgtgctcga gctgcgcgcg cgtgatcgcc agcgtcgcga ggttggcgcg
     1501 cgtgctgtcg agcagcgtct gcgcctgcag cacgtcggtc ttcgcggcga tggccgcggc
     1561 gtagcggtcc tgcgtgatct tcagcgagcg ctcgtagcct tcgacgctgc tgcgcgtcag
     1621 cgcgatctgc gcatcggcgg cgcgcagcgc gaagtagtcg gtcgcgagct cgccctgcgc
     1681 cgacagccgc gccgcggcga ggtcggccgc gctcgcttgc gcgctcgcct gcgcgccttc
     1741 gacgccgcgc cgcagcgcgc cccagatatc gggctcccag ctcgcgttgg cgccgagctg
     1801 caggcggttg ccggtgtcga gcgccgcacc gccgccgctg cgcgtggcgc tggcgttcag
     1861 accgatcgtc gggaacaacg tcgcgcgctg ttgcgcgacc aacgcgcgcg cctgtgcata
     1921 cgcggcgacg gctgcggcga cattctggtt cgacacctcg acctgcgccg cgagccggtt
     1981 cagttgcgca tcgccgaaca gctcccacca cgggccgcgc tccagcgtgt cggccggcgc
     2041 cgcggggaac caaccgggcg cgcctggcgg cgcctccttg aacgcggcag gcgccgcggc
     2101 actcgggcgc tcgtagtcgg gcccgaccgc acaggcggac agggccgcgg cggtcgcgat
     2161 cggcagcagc cagggccggg tgcgaagggt cggtcgtcgt tgcatggtca tcaggtcgat
     2221 ccggccagcg ccggcgggtc gttgccggcg cggctcaggt ggcgctcgtc ggcaccgcgg
     2281 cggcgcagcc ggtcgagcag caggtacacg accggcgtcg tcagcagcgt cagcacctgg
     2341 ctcgcgatca ggccgccgat gatcgcgatg cccagcggct ggcgcagctc ggagccttcg
     2401 ccgaaaccga tcgccagcgg cagcgcgcca agcgccgcgg ccagcgtcgt catcaggatc
     2461 ggacggaagc gcagcaggca ggcctcgcgc accgcttcga gtgaactcaa gccccgcgag
     2521 cgctcggcct cgagcgcgaa gtcgatgatc aggatcgcgt tcttcttgac gatgccgatc
     2581 agcaggaaca cgccgatcaa cgccatgatc gagaactcca tcttgaacag cagcagcgcc
     2641 agtaccgcgc cgacgccggc cgacggcagc gtgctgagca ccgtgaccgg gtgcaccagg
     2701 ctctcgtaga ggatgcccag cacgatgtag atcacgacga tcgcggcgac gatcagcaag
     2761 gtctgctggc tgttcgactg ctgcgccgac agcgcgctgc cggcgaagtc gccgcgcacc
     2821 gtggtgggca tgccgatgtt ggcctcggcg gtcttgatcg cgtcgcgcgc ctggtccagc
     2881 gtcacggcgt cggcgaggtt gaacgagatc gtggtcgcga gctcggtgtc ctggtggttc
     2941 accgaggtcg cggtcggctc ctcgacgaag cgcgccatcg tcgacagcgg caccaggctg
     3001 gtcgtggtgt tcgcgagaac gttgccggtc gacgcgctct tcaggccggg gttcgccgag
     3061 accgggacga cggtcgtcga cgcgcgcgac gacgtcgccg cgttcgccgc ggtgctctcg
     3121 accgccaccg cctggccgcc gctctcgacg atgcgcgtgg ccggcacgtg gatgtcggcc
     3181 agcgcgttcg ggctggtggt gtagccgggc gcccactcca tcacgacgtg gtactggttc
     3241 agctcggtgt agatcgtcgc gacctggcgc tggccgaagg cgttgtacag cgcggcgtcg
     3301 atcgcgctcg agctcacgcc caggcgtttc gccgcgtcgc ggtcgacctg gacggtggtc
     3361 tgcacgccgt tctcctgctg gtcggtgtcg acgtcggtca gctcgggctg ccgcttcatc
     3421 tcgtcgttga gcttcatcgt ccagttgcgc aggtcggcga cgttgtcgct cttcagcgtg
     3481 tactggtagg tcgagttgct cgagcggccg ccggagcgca cgtcctgcac cgtgttcagg
     3541 aacacctgca gcccggtcac gcgctgcagc tgcgggcgca gccgcgcgat caccgcgctg
     3601 cccttgtcgg tgcgctggcc gggcttggtg cccagcggct tcaggttcac gaacatgaag
     3661 ccgccgccgg cgcgcccgcc gccggtgaag c
//
LOCUS       DATMJB010000236         8128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_426013, whole genome shotgun sequence.
ACCESSION   DATMJB010000236 DATMJB010000000
VERSION     DATMJB010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426013"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2315
                     /gene="leuS"
                     /locus_tag="VNU48_05220"
     CDS             <1..2315
                     /gene="leuS"
                     /locus_tag="VNU48_05220"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HWI10710.1"
                     /translation="YAIDWTREFATCSPGYYVHEQRMFVRLMKQGLAYRRNSVVNWDP
                     VDQTVLANEQVEDGRGWRSGAIVEKREIPGYYLAITHYADELLANVADPTHPDYLHGW
                     PERVRLMQEHWIGKSEGVRFAFTHDIAGDDGEPIGGGRLWVFTTRADTIKGVTFCAVA
                     AEHPLALHAAARSPAVAAFVESCKAGGTTEAELALQEKRGIDTGLVVYHPLTDEQVPV
                     WIGNYVLMGYGDGAVMGVPAHDERDFAFAKKYGLDILQVIHVDGEPHFTYDHWQDWYA
                     LKDLSGDRAVTINSRNYNGMSYQVAVDAIAATLKRKGLGGKSTTWRLRDWGISRQRYW
                     GTPIPIIHCAKCGVVPVPEKDLPVVLPEDLIPDGSGNPLNKCAAFLNVACPSCGQPAR
                     RETDTMDTFVDSAWYFMRYTCPDAADAMVDARNAYWTPMDQYIGGIEHAVLHLLYARF
                     WTKAMRDLGLVDHGEPFTKLFTQGMLLNESFYRDDADGKRQWFYPSEVSVRYDDKGHP
                     VGATLIADGAPVALGGIEKMSKSKNNVVEPRDIIAKFGADTARLFTMFAGPPDQSAAW
                     SDSGAEGSYRYLRRLWNFAFKHADAIRTAGTELAGLSSGAQELRRDVHLLLRQISHDY
                     DRLQYNTVVSGAMKLLNALEGATLAATPADAAARREGVGILLRALYPAAPHITHALWR
                     ELGYAASFGELLDAPWPKVDDAALLQSEIELMLQVNGKLRGSIRVPADAGRHTIEVTA
                     LKSPDFIKFAEGKRAKKVIVVPGRLVNVVV"
     gene            2327..2818
                     /gene="lptE"
                     /locus_tag="VNU48_05225"
     CDS             2327..2818
                     /gene="lptE"
                     /locus_tag="VNU48_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HWI10711.1"
                     /translation="MTTRRHLLMAALLPLAGCGFELRRAPELKFRAIQFANFKPRSPL
                     AAELRTSINASTTTLVVEGPAQAQVVLEAIEDAREKVVVASGAVGQVTEFQLRARFKF
                     RLRSVSGRELIPETELLQNRDLSYTESAALAKEQEENFLYRAMQTDIVSQVMRRLASV
                     QTF"
     gene            2828..3925
                     /gene="holA"
                     /locus_tag="VNU48_05230"
     CDS             2828..3925
                     /gene="holA"
                     /locus_tag="VNU48_05230"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWI10712.1"
                     /translation="MQVRSDQLAAHLQKGLRPLYTVWGDEPLLAQEAGDAIRAAARAA
                     GCTERQVHTVAGAHFDWSGLLGASMAMSLFSERQLIEIRIPSGKPGKDGSEALQRYCE
                     HLGDEVVTLVQLPRLDRTQQGSAWFVALDAAGVTVRVDPIERKALPQWIAQRLAQQGQ
                     RVEGGAGGQRTLAFFADRVEGNLLAAHQEIQKLGLLYPAGELGFEQIESAVLNVARYD
                     VFKLGEAVLAGQAARALRMLAGLQAEGEAAVLVHWTLAEDIRALKRVKDAVAGGRPLP
                     IALREGRVWGAKERLFERVVPLLTEHAVAHLLEAAQVCDGLVKGLKHPDWPNDPWDGL
                     KRLVLMLVEHTGAAPAVGRGRVVVQRLALQA"
     gene            complement(4101..4592)
                     /locus_tag="VNU48_05235"
     CDS             complement(4101..4592)
                     /locus_tag="VNU48_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HWI10713.1"
                     /translation="MTRYEPLVLALAQALRAAGLRLATAESCTGGLIAAACTAVAGSS
                     DWFERGFVTYSNAAKTEAIGVDAALIAAHGAVSEEVALAMAQGALAHSPADLAVAVTG
                     IAGPGGATPGKPVGTVWVALARRGAPAQADLLQLQGDRAAVRAQTVEYALQRLLALAS
                     RAR"
     gene            complement(4589..5140)
                     /locus_tag="VNU48_05240"
     CDS             complement(4589..5140)
                     /locus_tag="VNU48_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HWI10714.1"
                     /translation="MNAPPGLNPPVGTAPRKPGWRFLLSHPAHAIALGFGSGLSPVAP
                     GTVGTLWAWIAFAVLQPHLNDARWALLLAVGALVGWWACTVAARHLAVADPGAIVWDE
                     ILAFWLILWLVTPASFWAQAWAFVLFRVFDAAKPGPVAWADQLFKVRRGMPIGWAQGF
                     GILFDDLVAALCTLLVIALWRFV"
     gene            complement(5137..6096)
                     /gene="thiL"
                     /locus_tag="VNU48_05245"
     CDS             complement(5137..6096)
                     /gene="thiL"
                     /locus_tag="VNU48_05245"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HWI10715.1"
                     /translation="MPLGEFDLIAKYFTRTVTRAALGVGDDCALFAPAPGMQLAVSSD
                     MLVEGRHFLPTVAPDRLGHKALAVNLSDLAACGAKPLGFTLALAMPRVDERFLDGFAR
                     GLLALADAHDCELIGGDTTQGPLNLCITVFGELPHGQALLRSGARAGDDLYVSGLLGD
                     ARLALEVFRGTVALAGDAFERVRVAMEQPQPRVALGLALRGIATSAIDVSDGLLGDLA
                     HVLRRSGVGAELQVDALPRSDVLRTLPEALQRDCTLAGGDDYELVFSAAPGDAARVAA
                     AARSAQVAVTRIGRLTADLGLRLIDRDGRPVAHTFGSFDHFKS"
     gene            6304..>8128
                     /locus_tag="VNU48_05250"
     CDS             6304..>8128
                     /locus_tag="VNU48_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HWI10716.1"
                     /translation="MSTNAEQKAAELRRDALDYHEFPTPGKVAIHATKQMLNQRDLAL
                     AYSPGVAHACEEIVADPANAFRYTARGNLVAVITNGTAVLGLGNIGPLAAKPVMEGKG
                     VLFKKFAGIDVFDIEIAETNLDKLIDHIAALEPTFGGINLEDIKAPDCFYVERALRQR
                     MKIPVFHDDQHGTAIVVGAGLLNALKVSGKNIKEVKLVTSGAGAAALACLGLLMKLGL
                     PRENIWVTDLAGVVYQGRTELMDEDKIGFAQKTDARTLADVIKGADIFLGLSAGGVLK
                     KNMVASMAPQPMIFALANPTPEILPEEVLEVRSDAIIATGRTDYPNQVNNVLCFPYIF
                     RGALDSGATTITIEMEIAAVHAIAELAQAEQSEVVAAAYAGVNLSFGPEYLIPKPFDP
                     RLMMKIAPAVAQAAADSGVAARPIADMDAYRDKLQTFVYASGTTMKPIFMLAKQALNK
                     RVAFAEGEEERVLRAVQIVVDEGLARPTLIGRPEVIAQRIEKFGLRLAAGTDYDIVNT
                     ENDHRFRDYWQSYHRLTERKGVTAQLAKIEMRRRLTLIGSMLLKTGEVDGMLCGTWGT
                     TAMHLHYIDQVIGKREGVCTYACMNGLMLPGRQVFLVDTHVN"
BASE COUNT         1339 a         2854 c         2869 g         1066 t
ORIGIN      
        1 gctacgcgat cgactggacg cgcgaattcg cgacctgcag ccccggctat tacgtgcacg
       61 agcagcgcat gttcgtgcgg ctgatgaagc agggcctggc gtaccgccgc aactcggtcg
      121 tgaactggga cccggtcgac cagaccgtgc tcgccaacga gcaggtcgag gacgggcgcg
      181 gctggcgcag cggcgcgatc gtcgagaagc gcgagattcc gggctactac ctggcgatca
      241 cgcactacgc cgacgagctg ctggccaacg tcgccgaccc gacccacccg gactacctgc
      301 acggctggcc cgagcgcgtg cggctgatgc aggagcactg gatcggcaag agcgagggcg
      361 tgcgcttcgc gttcacccac gacatcgccg gcgacgacgg cgagccgatc ggcggtggcc
      421 ggctgtgggt cttcacgacc cgcgccgaca cgatcaaggg cgtgaccttc tgcgccgtcg
      481 cggcggagca tccgctcgcg ctgcacgcgg ccgcgcgcag cccggcggtg gccgcgttcg
      541 tcgaatcgtg caaggccggc ggcacgaccg aagccgaact cgcgttgcag gaaaagcgcg
      601 gcatcgacac cgggctggtc gtctaccacc cgctgaccga cgagcaggtc ccggtctgga
      661 tcggcaacta cgtgctgatg gggtacggcg acggtgccgt gatgggcgtg ccggcgcacg
      721 acgagcgcga cttcgcgttc gcgaagaaat acggcctcga catcctgcag gtgatccatg
      781 tcgacggcga accgcacttc acctacgacc actggcagga ctggtacgcg ctgaaggacc
      841 tcagcgggga ccgcgcggtc acgatcaact cgcgcaacta caacggcatg tcgtaccagg
      901 tcgcagtcga tgcgatcgcc gcgacgctga agcgcaaggg tctcggcggc aagtcgacga
      961 cctggcgcct gcgcgactgg ggcatcagcc gccagcgcta ctggggcacg ccgatcccga
     1021 tcatccactg cgcgaaatgc ggcgtggtgc cggtgcccga gaaggacctg ccggtcgtgc
     1081 tgcccgagga tctgatcccc gacggttcgg gcaacccgtt gaacaaatgc gcggcgttcc
     1141 tgaacgtcgc gtgcccgagc tgcggccagc cggcgcggcg cgagaccgac acgatggaca
     1201 ccttcgtcga ttcggcctgg tacttcatgc gctacacctg cccggacgcg gccgacgcga
     1261 tggtcgatgc gcgcaacgcc tactggacgc cgatggacca gtacatcggc ggcatcgagc
     1321 acgcggtgct gcacctgctg tacgcgcgct tctggaccaa ggcgatgcgc gacctcgggc
     1381 tggtcgacca cggcgagccg ttcacgaagc tgttcacgca gggcatgctg ctgaacgagt
     1441 cgttctaccg cgacgacgcc gacggcaagc ggcagtggtt ctaccccagc gaggtcagcg
     1501 tccgctacga cgacaagggc cacccggtcg gtgcgaccct gatcgccgac ggcgcgccgg
     1561 tggcgctggg cggcatcgag aagatgtcga agtcgaagaa caacgtggtc gagccgcgcg
     1621 acatcatcgc caagttcggc gccgacaccg cgcggctgtt cacgatgttc gccggcccgc
     1681 cggaccagag cgcggcctgg tccgattcgg gcgccgaggg ttcgtaccgc tacctgcggc
     1741 gcttgtggaa tttcgcgttc aagcacgccg acgcgatccg caccgcgggc accgagcttg
     1801 cgggcctgag cagcggcgcg caggagctgc gccgcgacgt ccacctgctg ctgcgccaga
     1861 tcagccacga ctacgaccgg ctgcaataca acaccgtggt ctcgggcgcg atgaagctgc
     1921 tgaacgcgct cgaaggggcg acgctcgcgg ccacgcccgc cgacgccgcg gcgcggcgcg
     1981 aaggtgtggg catcctgctg cgcgcgctgt acccggccgc gccgcacatc acgcacgcgc
     2041 tgtggcgcga actcggctac gccgcgagct tcggtgagct gctcgacgcc ccctggccga
     2101 aggtcgacga cgcggcgctg ctgcaaagcg agatcgagct gatgctgcag gtcaacggca
     2161 agctgcgcgg cagcatccgc gtgcccgccg acgccggccg gcacacgatc gaggtgaccg
     2221 cgctgaagag ccccgacttc atcaagttcg cggaaggcaa gcgcgcgaag aaggtgatcg
     2281 tcgtaccggg ccgcctcgtc aacgtcgtcg tctgaagatg agcacgatga cgacccgccg
     2341 ccatctcctc atggcggcgc tgctgccgct ggcgggttgc ggcttcgagt tgcggcgcgc
     2401 gccggagctg aagttccgcg cgatccagtt cgccaacttc aagccgcgct cgccgctggc
     2461 cgccgagctg cgcaccagca tcaacgcgag cacgacgacg ctggtcgtcg agggcccggc
     2521 gcaggcgcag gtcgtgctcg aggcgatcga ggacgcgcgc gagaaggtgg tcgtcgccag
     2581 cggcgcggtc ggccaggtca cggagttcca gctgcgcgcg cgcttcaagt tccgcctgcg
     2641 cagcgtgagc ggccgcgagc tgatccccga gaccgagctg ctgcagaacc gcgacctgag
     2701 ctacaccgag agtgcggcgc tcgcgaagga acaggaagag aacttcctgt accgcgcgat
     2761 gcagaccgac atcgtctcgc aggtgatgcg gcgcctggcc tcggtgcaga cgttctgatc
     2821 gggcgcgatg caagtccgct ccgaccagct cgccgcgcac ctgcagaagg gcctgcgccc
     2881 gctctacacg gtgtggggcg acgagccgct gctggcgcag gaggccggcg atgcgatccg
     2941 cgccgccgcg cgcgccgccg gctgcaccga acgccaggtg cacacggtgg ccggcgcgca
     3001 cttcgactgg agcgggctgc tcggcgcgtc gatggcgatg agcctgttct ccgagcgcca
     3061 gctgatcgag atccgcattc cgtcgggcaa gcccggcaag gacggctccg aagcgctgca
     3121 acgctattgc gagcacctcg gcgacgaggt cgtcacgctg gtgcagttgc cgcggctcga
     3181 ccgcacgcag cagggcagcg cgtggttcgt cgcgctcgac gccgccggcg tcacggtgcg
     3241 cgtcgatccg atcgagcgca aggccctgcc gcaatggatc gcgcaacgcc tggcgcagca
     3301 aggccagcgc gtcgaagggg gcgcgggcgg ccagcgcacg ctcgcgttct tcgccgaccg
     3361 cgtcgaaggc aacctgctgg ctgcgcacca ggagatccag aagctcgggc tgctctaccc
     3421 ggccggcgaa ctcggcttcg agcagatcga atcggcggtg ctgaacgtgg cgcgctacga
     3481 cgtcttcaag ctcggcgagg cggtgctcgc gggccaggcg gcgcgcgcgc tgcgcatgct
     3541 cgccgggctg caggccgaag gcgaggcggc ggtgctggtg cactggacgc tggccgaaga
     3601 catccgcgcg ctcaagcgcg tgaaagacgc ggtcgccggc ggcaggccgc tgcccatcgc
     3661 actgcgcgag ggtcgcgtct ggggcgcgaa ggagcggctg ttcgaacgcg tcgtgccgct
     3721 gctgaccgag cacgcggtcg cgcacctgct cgaagcagcg caggtctgcg acgggctcgt
     3781 caagggcctg aagcacccgg actggccgaa cgatccctgg gacggcctga agcgtctggt
     3841 gctgatgctg gtcgagcaca ccggggccgc cccggccgtc ggccgcgggc gcgtcgtcgt
     3901 gcagcggctc gcgctgcagg cgtgagcctg agcgtgcgtc agtagccgcg cgttcgatcg
     3961 accaggtgct cgagcggccg acccgcgcgc agcgcgagca cgttgcgcgc cgcgtgccag
     4021 gttcaccagg cttgccccgc ggggcggctg tgcgaggcgc gcggcgtcga acgcggcggc
     4081 gaccgggatc gccatcgccc tcagcgcgcc cgcgacgcca gcgccagcaa gcgctgcagc
     4141 gcgtactcga cagtctgcgc gcggaccgcc gcacggtcgc cctgcagctg cagcagatcg
     4201 gcctgcgcgg gcgccccgcg gcgcgccagg gcgacccaga ccgtgccgac cggcttgccg
     4261 ggcgtcgcac cgcccggccc ggcgatgccg gtgacggcga ctgcgagatc ggccggcgaa
     4321 tgcgccagcg caccctgcgc catcgcaagc gcgacttcct cgctgaccgc gccgtgcgcg
     4381 gcgatcagcg ccgcgtcgac accgatcgcc tcggtcttcg ccgcgttcga gtaggtcacg
     4441 aagccgcgtt cgaaccagtc gctcgacccg gccacggccg tgcaggccgc ggcgatcagc
     4501 ccgccggtgc aggactcggc cgtcgccagg cgcagaccgg ccgcgcgcag ggcctgcgcg
     4561 agcgccagca cgagcggctc gtagcgcgtc atacgaagcg ccacagcgcg atcacgagca
     4621 gcgtgcacag cgccgcgacc aggtcgtcga acaggatgcc gaagccctgc gcccagccga
     4681 tcggcatgcc gcggcgcacc ttgaagagct gatcggccca cgcgaccggg ccgggcttgg
     4741 ccgcgtcgaa gacgcggaac agcacgaagg cccaggcctg tgcccagaag ctcgccggcg
     4801 tcaccagcca caggatgagc cagaacgcga ggatctcgtc ccagacgatc gcgccggggt
     4861 cggcgaccgc gaggtggcgc gccgcgaccg tgcaggccca ccagcccacc agcgcgccga
     4921 cggccagcag cagcgcccag cgcgcgtcgt tcaggtgcgg ctgcagcacc gcgaacgcga
     4981 tccaggccca cagggtgccg accgtgcccg gcgcgaccgg cgacagcccg ctgccgaagc
     5041 cgagcgcgat cgcatgcgcc gggtgcgaca gcaggaagcg ccagcccggc ttgcgcggcg
     5101 cggtgccgac cggcgggttc agacccgggg gcgcattcat gatttgaagt ggtcgaacga
     5161 gccgaaggtg tgggccaccg ggcggccgtc gcggtcgatc aagcgcaggc cgaggtcggc
     5221 ggtaaggcgc ccgatgcgcg tgaccgcgac ctgcgccgat cgagccgcgg cggcgacccg
     5281 tgcggcgtcg cccggtgcgg cactgaacac gagttcgtaa tcgtcgccgc cggcgagcgt
     5341 gcagtcgcgc tgcagtgcct cgggcagcgt gcgcagcacg tcgctgcgcg gcagcgcatc
     5401 gacctgcagc tcggcgccga cgccggagcg ccgcagcacg tgcgcgaggt cgccgagcag
     5461 gccgtcggag acgtcgatcg cgctcgtcgc gatgccgcgc agcgccaggc cgagcgcgac
     5521 gcgcggctgc ggctgctcca tcgccacgcg cacgcgctcg aacgcgtcgc cggccagcgc
     5581 gaccgtgccg cggaagactt cgagcgcgag ccgcgcatcg ccgagcaggc cgctgacgta
     5641 caggtcgtcg ccggcgcgcg cgcccgagcg cagcagcgcc tgaccgtgcg gcagctcgcc
     5701 gaagacggtg atgcacaggt tcagcgggcc ctgcgtggtg tcgccgccga tcagctcgca
     5761 gtcgtgtgcg tcggccagtg ccagcaggcc gcgcgcaaag ccgtcgagga agcgctcgtc
     5821 gacccgcggc atcgccagcg ccagcgtgaa cccgagcggc ttcgcgccgc aggccgccag
     5881 gtcgctcagg ttcaccgcga gcgccttgtg gccgagccgg tcgggcgcga cggtcggcag
     5941 gaagtgccgg ccctcgacca gcatgtcgct cgataccgcg agctgcatgc cgggcgcggg
     6001 ggcgaacaac gcgcagtcgt cgccgacccc gagcgcggcg cgcgtcacgg tgcgggtgaa
     6061 gtacttcgcg atcaggtcga attcgccgag gggcatgggc gtgggcgccg ctgtgggtga
     6121 atggaaatgc cggccgtcgc cggccctggc atgcggcgca taccaaccgt cgatgccgca
     6181 tcggcattat cggtggggcc cgcgcggcca cctttgttcg acgccggact tctacaatcg
     6241 accccccgcc cgcgcatcga ttcgcgcgtc gggcggatcc gacgaacaca ccgagacaag
     6301 tccatgtcca cgaacgccga acaaaaggcc gccgaactgc gccgcgacgc cctcgactat
     6361 cacgagttcc cgacgcccgg caaggtcgcg atccacgcga ccaagcagat gctgaaccaa
     6421 cgcgacctgg ccttggccta ctcgcccggc gtcgcccatg cctgcgaaga gatcgtggcc
     6481 gacccggcga acgcgttcag gtacaccgcg cgcggcaacc tggtggcggt gatcacgaac
     6541 ggcaccgcgg tgctggggct gggcaacatc ggcccgctgg cggccaagcc ggtgatggaa
     6601 ggcaagggcg tgctgttcaa gaagttcgcc ggcatcgacg tgttcgacat cgagatcgcc
     6661 gagaccaacc tcgacaagct gatcgaccac atcgcggcgc tcgagccgac cttcggcggc
     6721 atcaacctcg aggacatcaa ggcgccggac tgcttctacg tcgagcgcgc gctgcgccag
     6781 cgcatgaaga tcccggtgtt ccacgacgac cagcacggca ccgcgatcgt cgtcggcgcc
     6841 gggctgctga acgcgctgaa ggtctcgggc aagaacatca aggaagtcaa gctcgtgacc
     6901 tcgggcgccg gcgccgccgc gctcgcctgc ctgggcctgc tgatgaagct cggcctgccg
     6961 cgcgagaaca tctgggtcac cgacctggcc ggcgtggtct accagggccg caccgagctg
     7021 atggacgaag acaagatcgg ctttgcgcag aagaccgatg cgcgcacgct cgccgacgtg
     7081 atcaagggcg ccgacatctt cctcggcctg tcggccggcg gcgtgctgaa gaagaacatg
     7141 gtcgcgagca tggcgccgca gccgatgatc ttcgcactgg cgaacccgac gcccgagatc
     7201 ctgcccgaag aggtgctcga ggtgcgcagc gacgcgatca tcgcgaccgg ccgcaccgac
     7261 tacccgaacc aggtcaacaa cgtcctgtgc ttcccgtaca tcttccgcgg cgcgctcgat
     7321 tcgggcgcga ccacgatcac gatcgagatg gagatcgccg cggtccacgc cattgccgaa
     7381 ctcgcgcagg ccgagcagag cgaggtggtg gcggccgcct atgccggcgt gaacctcagc
     7441 ttcgggccgg agtacctgat ccccaagccg ttcgacccgc ggctgatgat gaagatcgcg
     7501 ccggccgtgg cgcaggccgc cgccgactcg ggcgtggcgg cgcgcccgat cgccgacatg
     7561 gacgcgtacc gcgacaagct gcagaccttc gtctacgcgt cgggcacgac gatgaagccg
     7621 atcttcatgc tcgccaagca ggcgctgaac aagcgcgtcg ccttcgccga gggcgaagaa
     7681 gaacgcgtgc tgcgcgcggt gcagatcgtc gtcgacgaag gcctggcgcg tccgacgctg
     7741 atcggacgcc ccgaggtgat cgcgcagcgc atcgagaagt tcggcctgcg cctggcggcg
     7801 ggcaccgact acgacattgt caacaccgag aacgaccatc gcttccgcga ctactggcaa
     7861 agctaccacc gcctgaccga gcgcaagggc gtgacggcgc agctggcgaa gatcgagatg
     7921 cgccgccgcc tgaccttgat cggctcgatg ctgctcaaga ccggcgaggt cgacggcatg
     7981 ctgtgcggca cctggggcac gaccgcgatg catctgcact acatcgacca ggtgatcggc
     8041 aagcgcgaag gcgtctgcac ctatgcctgc atgaacggcc tgatgctgcc gggccgccag
     8101 gtgttcctgg tcgataccca cgtcaact
//
LOCUS       DATMJB010000237         2475 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_248206, whole genome shotgun sequence.
ACCESSION   DATMJB010000237 DATMJB010000000
VERSION     DATMJB010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248206"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VNU48_05255"
     CDS             <1..391
                     /locus_tag="VNU48_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWI10717.1"
                     /translation="DDHDHDHDHHDHDHDHEHGEHCDHPHHHAHDDDVKSFVFRSDKA
                     FNPAKLEDFLGAIVQVYGPKMLRYKGVLNMKGSDRKVIFQGVHQLMGSDLGPKWAPGE
                     KKTNKMVFIGLDLPREVFLQGLEGCLV"
     gene            523..1452
                     /gene="dksA"
                     /locus_tag="VNU48_05260"
     CDS             523..1452
                     /gene="dksA"
                     /locus_tag="VNU48_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HWI10718.1"
                     /translation="MSKTPAPKAPAKKTAAGKAAHAASASSKGAKAGAKAGAKATPKT
                     AKVAAPVPKKSVAPAQKAAPASKKAAPAPAAAKPVVAARPPTITAAPPPVVALVGGLA
                     PMTPRKSGRGASKYPAPAVQAPLPPPTSRFADRFAPPRPPTRQMNNLELDTPREKQKF
                     DPKLANAWKSKSGRELSEAEILAMPESEYMNDKQLEFFRVKLQTLKDDLLSNAGETTE
                     HLREDTSIVPDPADRATIEEEHALELRTRDRERKLLKKISQSLQRIDAGDYGFCDETG
                     EPIGLGRLIARPTATLSLEAQQRRELKQKMFGD"
     gene            1522..>2475
                     /locus_tag="VNU48_05265"
     CDS             1522..>2475
                     /locus_tag="VNU48_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10719.1"
                     /translation="MSEPKDTTSFFRKVVKFVANPTTEWAELDAPAEEARETEFAKGE
                     LKAMIERKRRNDFVRKREFDMLRKVRREGLTSEQLAALGGSSRIDDSEAKMVESGAKA
                     EVGVKAKIDEIEQQMVGDAPYAQHSGMRRTPEFYNAPTVPAAMDARPVRPSSRSHFDG
                     AQVDEVMAERATPMAVSPVVMGAGVSPLSPVRSDADPVHAAGRALSEIAHDPELDEAV
                     IAFANADFEQCEQALQTLTGPGGARSQHADTWLVLFDLYRAIGQQHKFESLALDYAQQ
                     FGWSAPQWFSLPKLVAEAASGDRPRRPHIEGQVGWVSPEHVD"
BASE COUNT          478 a          884 c          816 g          297 t
ORIGIN      
        1 cgacgaccac gatcacgacc acgatcacca cgaccacgac cacgaccacg agcacggcga
       61 acactgcgat cacccgcacc accacgcgca cgacgacgac gtgaagtcgt tcgtgttccg
      121 ctccgacaag gccttcaacc cggccaagct cgaagacttc ctcggcgcga tcgtccaggt
      181 ttacgggccg aagatgctgc gctacaaggg cgtgctgaac atgaaaggca gcgaccgcaa
      241 ggtgatcttc cagggcgtgc accagctgat gggcagcgac ctgggcccga agtgggcgcc
      301 gggcgagaag aagaccaaca agatggtgtt catcggcctc gatctgccgc gcgaagtctt
      361 cctgcaaggc ctcgagggct gcctcgtctg aggcctgcga cacgagcgga tgcgcagcat
      421 gaattccaca cggcgagccg cggtgcaagc tggctacaat ccgcggcggc cgaagccccg
      481 cccctgtgca cagggtataa ccgccgtcgc gaggagaacg ctgtgagcaa gaccccggcc
      541 ccgaaagcac ccgccaagaa gaccgccgcc ggcaaggccg cccacgctgc atcggcttcg
      601 agcaagggtg ccaaggccgg cgcgaaggcc ggcgcaaaag ccacgcccaa gaccgccaag
      661 gtcgcggcgc cggtgccgaa gaagagcgtg gcccccgcgc agaaggcggc gccggcatcg
      721 aagaaggcgg cgccggcgcc cgccgccgcc aagccggtcg tggcggccag gccgccgacg
      781 atcaccgccg cgccgccgcc ggtcgtcgcg ctggtgggcg gcctggcgcc gatgacgccg
      841 cgcaagtccg gccgcggcgc ttccaagtac cccgcgccgg cggttcaggc cccgttgccg
      901 ccgccgacct cccgattcgc cgaccggttt gcaccgccgc ggcctccgac ccgacagatg
      961 aacaacctcg aactcgacac cccgcgtgag aagcagaaat tcgatcccaa gctcgccaac
     1021 gcctggaagt ccaagagcgg ccgcgagctg agcgaggccg aaatcctggc gatgccggaa
     1081 tcggagtaca tgaacgacaa gcagctcgag ttcttccgcg tcaagctgca gacgctgaag
     1141 gacgacctgc tcagcaacgc cggcgagacc accgagcacc tgcgcgagga cacctcgatc
     1201 gtgcccgacc cggccgaccg cgcgaccatc gaggaagagc atgcgctgga actgcgcacc
     1261 cgcgaccggg aacgcaagtt gctcaagaag atctcgcaat cgctgcagcg catcgacgcc
     1321 ggcgactatg gcttctgtga cgaaaccggc gaacccatcg gcctgggccg cctgattgcc
     1381 cggcccaccg ctacactgtc cctcgaagcc cagcagcggc gcgaactcaa gcagaagatg
     1441 ttcggcgatt gagcccctcc ggcctgcggc gcgcgggccg gaccttgtga atcccagccc
     1501 aggagcccgc ggccccgcga catgtcggaa ccgaaagaca cgacaagctt cttccgcaag
     1561 gtcgtcaagt tcgtcgccaa cccgaccacg gagtgggccg aactcgacgc gcccgccgag
     1621 gaagcgcgcg agaccgagtt cgccaagggc gaactgaagg ccatgatcga gcgcaagcgg
     1681 cgcaacgatt tcgtacgcaa gcgcgagttc gacatgcttc gcaaggtgcg ccgcgagggc
     1741 ctgaccagcg agcagctcgc cgcgctcggc ggttcgtcgc gcatcgacga ctccgaagcc
     1801 aagatggtcg agagcggcgc caaggccgag gtcggcgtca aggccaagat cgacgagatc
     1861 gaacagcaga tggtgggcga tgcgccgtac gcgcagcaca gcggcatgcg ccgcacgccc
     1921 gagttctaca acgcacccac agtgccggcg gcgatggatg cgcgcccggt gcgcccgtcc
     1981 tcgcgcagcc atttcgacgg cgcgcaggtc gacgaggtga tggccgagcg cgcaacgccg
     2041 atggcggtct cgccggtcgt gatgggcgcc ggcgtctcgc cgctgtcgcc ggtcaggtcc
     2101 gacgccgatc cggtgcatgc ggcgggccgc gcgctgagcg agatcgcgca cgaccccgaa
     2161 ctcgacgagg ccgtgatcgc gttcgcgaac gccgatttcg agcaatgcga gcaggccctg
     2221 cagacgctga ccggccccgg cggcgcgcgc tcgcagcacg ccgacacctg gctggtgctg
     2281 ttcgacctgt accgcgcgat cggccagcag cacaagttcg agagcctcgc gctcgactat
     2341 gcgcagcagt tcggctggtc ggcgccgcag tggttctcgc tgcccaagct cgtcgccgaa
     2401 gccgccagcg gcgaccggcc gcgccgcccg cacatcgagg gccaggtcgg ctgggtcagc
     2461 ccggagcacg tcgat
//
LOCUS       DATMJB010000238         3320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_679496, whole genome shotgun sequence.
ACCESSION   DATMJB010000238 DATMJB010000000
VERSION     DATMJB010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679496"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /gene="fliQ"
                     /locus_tag="VNU48_05270"
     CDS             <1..261
                     /gene="fliQ"
                     /locus_tag="VNU48_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HWI10720.1"
                     /translation="TQVFTAGQQGLYILLLVSAPMLISVLLIGLVVSIFQAATQIHEA
                     TLSFVPKIIGAVAVLAIAGPWMLTTLVEYIQRTLQAIPSAVG"
     gene            293..1054
                     /gene="fliR"
                     /locus_tag="VNU48_05275"
     CDS             293..1054
                     /gene="fliR"
                     /locus_tag="VNU48_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HWI10721.1"
                     /translation="MLTFTDTQVLAWITPLLWPFLRVLALFGSLPVFAQRGVPARVRV
                     ALAFLIAFCAQATLPTMPLIPLDSAAAVLAVVQQLLIGLSLGFAVRIVFAAVEFAGEM
                     IGLQMGLNFAGFFNPMTGGEATATSRFFSIAVSWLFIVIGGHLMLIAAVVQSFDAFPV
                     GPEPFAFLRAVQPQVWGAEVFRLGLWIALPMIAMLLFVNLVLGIISRVAQQMSIFSIG
                     FPITVSVGLVGMLLTLPMMQMPFTMALERMLQYFR"
     gene            1203..>3320
                     /locus_tag="VNU48_05280"
     CDS             1203..>3320
                     /locus_tag="VNU48_05280"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWI10722.1"
                     /translation="MIAGSATLAPPPGVVARVSSPLVRRLSKLSVGKKLLLIYLLDLS
                     TVFFISSILIHEKYIAIDFSRKELAGTAYIEALRPTLVAIARSAPAPAIDGPVLEALS
                     RAEQQHGDGMGSAELNGKFSLALQALPGRATPREAAARRQQALEQGRELVTRVGNQSN
                     LILDPDLDSYYTMSLILLRYPTLLDVVAQITPRLHDVAGGGAIDPAERTRLFVLEGEL
                     RTAANAIQSDYAEAVAASPPLLGVRLSPPQAELAAAIDRLRAVVRQAAAGDSVERHQI
                     DATEHAVLDALDLAWGRGNGELTRLIDLRVHDFFVRMWVHLGTAAGLLMFILVAVYYV
                     AKRISRPLRALSQVVDTVRRTGDHTLRSHWDSDDEIGRLVLGFNEMLAQLDHQRQVQQ
                     EMAASARAAEAQQRLLELMPVPLMVTSVPDHRVLHANPQAREWLGDTDADPWAAGISS
                     ELRRRFFQQLADQGVVHEFEVRWQHGTHAAWAVLSARRLDYQGQDAVVTAFTPVNNLK
                     ALEQRLELWAKVFEASSEGIVLLDANGRVLSANRAICKAGAFDPVELLHEPLAFVHVD
                     DQPMPQARATWATAESRGWWRGEVQVHKRDGGSFPAWAVLTAVRDATGVVGHYIFSCL
                     DISDRKASEEQVRYLAHHDLLTGLPNRGVAERRLRQAMQESTRSGQPVGVLFIDLDRF
                     KTINDSLGHQTGDALLKVVAQRLR"
BASE COUNT          453 a         1169 c         1156 g          542 t
ORIGIN      
        1 acccaagtct tcaccgccgg ccagcagggc ctgtacatcc tgctgctggt ctcggcgccg
       61 atgctgatca gcgtgctgct gatcggcctg gtcgtcagca tcttccaggc cgccacgcag
      121 atccacgagg cgacgctgtc gttcgtgccg aagatcatcg gcgcggtcgc ggtgctcgcg
      181 atcgccgggc cctggatgct gacgacgctg gtcgagtaca tccagcgcac gctgcaggcg
      241 atcccgagcg ccgtcggcta gtcgccgtgg gccgaccgca ggagttcgcg cggtgctgac
      301 cttcaccgac acccaggtcc tggcctggat cacgccgctg ctgtggccgt tcctgcgcgt
      361 gctcgcgctg ttcggctcgc tgccggtgtt cgcgcagcgc ggcgtgccgg cgcgcgtgcg
      421 cgtcgcgctc gcgttcctga tcgcgttctg cgcgcaggcc acgctgccga cgatgccgct
      481 gatcccgctc gactcggccg ccgcggtgct cgcggtggtg cagcagttgc tgatcggcct
      541 gtcgctgggc ttcgcggtgc gcatcgtgtt cgccgcggtc gagttcgcgg gcgagatgat
      601 cgggctgcag atgggcctga acttcgccgg cttcttcaac ccgatgaccg gcggtgaggc
      661 caccgcgacg agccgcttct tcagcatcgc ggtgagctgg ctgttcatcg tcatcggcgg
      721 ccacctgatg ctgatcgccg cggtggtgca gagcttcgac gcgtttccgg tcggccccga
      781 gccgttcgcg ttcctgcgcg cggtgcagcc gcaggtctgg ggcgccgagg tgttccgcct
      841 cggcctgtgg atcgcgctgc cgatgatcgc gatgctgctg ttcgtcaacc tggtgctggg
      901 catcatctcg cgcgtcgcgc agcagatgag catcttctcg atcggctttc cgatcaccgt
      961 ctcggtcggc ctggtcggca tgctgctgac cctgccgatg atgcagatgc cgttcacgat
     1021 ggcgctggaa cggatgcttc agtacttccg ctgaccaacg cggcacgcgg cggcgctcaa
     1081 gttccaggcc gcgcggccga agagcctttc gcagggtcca tgttgcatcg caccaaatgc
     1141 gggcatcgcg gcacccgaac gccaccgcgg ccccggccga ttctcttcgg aggagtacac
     1201 cgatgattgc tggttctgcc accctggctc ccccgcccgg cgtcgtcgcc cgggtcagct
     1261 cgccgctggt gcgccggctg tcgaaactga gcgtcggcaa gaagctgctg ctgatctacc
     1321 tgctcgacct gagcacggtg ttcttcatca gcagcatcct gattcacgag aagtacatcg
     1381 cgatcgattt ctcgcgcaag gagctcgcag gcacggccta catcgaggcg ctgcgtccga
     1441 cgctggtggc gatcgcgcgc tcggccccgg cgccggcgat cgacggcccg gtgctggagg
     1501 cactctctcg cgccgagcag cagcacggcg acggcatggg cagcgcggag ctgaacggca
     1561 agttctcgct cgccctgcag gcgctgccgg gccgcgccac gccgcgcgag gcggcggcac
     1621 gacgccagca ggcgctcgag cagggccgcg agctggtcac gcgggtgggc aaccagtcga
     1681 acctgatcct cgaccccgac ctggacagct actacacgat gtcgctgatc ctgctgcgct
     1741 accccacgct gctcgatgtg gtggcgcaga tcacgccgcg gctgcacgat gtcgctggcg
     1801 gcggcgcgat cgacccggcc gagcgcaccc ggctgttcgt gctcgaaggc gagctgcgca
     1861 ccgcggccaa cgcgatccag tccgactatg ccgaggcggt ggcggcgtcg ccgccgctgt
     1921 tgggcgtgcg gctgtcgccg ccccaggccg aactcgccgc cgcgatcgat cgcctgcgcg
     1981 ccgtggtgcg ccaggcggcc gcgggcgact cggtcgaacg ccaccagatc gacgccaccg
     2041 aacatgccgt gctcgacgcg ctcgacctcg cctggggccg cggcaacggc gagctgacgc
     2101 gcctgatcga tttgcgcgtg cacgatttct tcgtccgcat gtgggttcac ctggggacgg
     2161 cggccggtct gctgatgttc atcctggtgg ccgtctacta cgtcgcgaag cgcatctcgc
     2221 ggccgttgcg cgcgctgtcg caggtcgtcg acacggtgcg ccgcaccggc gaccacacgc
     2281 tgcgctcgca ttgggacagc gacgacgaga tcggccgcct ggtgctgggc ttcaacgaga
     2341 tgctcgccca gctcgaccac cagcgccagg tccagcagga gatggcggcc agcgcacggg
     2401 ccgccgaggc acagcagcgc ctgctcgagc tgatgccggt gccgctgatg gtgacctcgg
     2461 tgcccgatca tcgcgtgctg cacgccaacc cgcaggcgcg cgagtggctc ggcgacaccg
     2521 acgccgaccc gtgggccgcc ggcatcagca gcgaactgcg gcgccgcttc ttccagcagc
     2581 ttgccgacca gggggtcgtg cacgagttcg aggtgcgctg gcagcacggc acgcacgcgg
     2641 cctgggcggt gctgtcggcg cggcgcctgg actaccaggg ccaggacgcg gtcgtcaccg
     2701 cgttcacgcc ggtcaacaac ctgaaggcgc tcgagcagcg gctcgaactc tgggccaagg
     2761 tgttcgaggc ctcgtcggaa ggcatcgtgc tgctcgacgc gaacggccgg gtgctgtcgg
     2821 cgaaccgcgc gatctgcaag gccggcgcct tcgaccccgt cgagctgctg cacgagccgc
     2881 tggcgttcgt gcacgtcgac gaccagccga tgccgcaggc gcgcgccacc tgggccaccg
     2941 ccgagagccg cggctggtgg cgcggcgagg tgcaggtgca caagcgcgac ggcggttcgt
     3001 ttccggcctg ggccgtgctg accgcggtgc gcgacgcgac cggcgtggtc gggcactaca
     3061 tcttcagctg cctggacatc agcgaccgca aggcgtcgga ggagcaggtg cgctacctgg
     3121 cccaccacga cctgctcacg ggcctgccga accggggcgt cgccgagcgg cggctgcgcc
     3181 aggcgatgca ggaatcgacg cgcagcggtc agccggtcgg cgtgctgttc atcgacctcg
     3241 accgcttcaa gaccatcaac gactcgctcg gccaccagac gggcgacgcg ctgctcaagg
     3301 tggtggcgca gcgcctgcgc
//
LOCUS       DATMJB010000239         6840 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_638623, whole genome shotgun sequence.
ACCESSION   DATMJB010000239 DATMJB010000000
VERSION     DATMJB010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6840
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638623"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1019)
                     /gene="hprK"
                     /locus_tag="VNU48_05285"
     CDS             complement(72..1019)
                     /gene="hprK"
                     /locus_tag="VNU48_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HWI10723.1"
                     /translation="MKPSVISAEALFEEHREILHWEWIAGHAHPERRFDEAAVRSAQS
                     AADLVGYLNYIHPYRVQIVGRREVAYLSVAPEVAERRISRIVTLEPPVLIVADGETPP
                     DKLVALCDRADIPLFATGESAGHVIDVVRAYLGLHFAERTTRHGVFMDILGVGVLITG
                     ESGLGKSELGLELISRGHGLVADDAVDLYRVSQTGLEGRCPDLLLNLLEVRGIGLLDI
                     KAIFGETAVRRKMRLKLIVHLVRKETMDREFERLPYEPLNEEILDVPVRKTVIAVDAG
                     RNLAVLVEAAVRNTILQLRGIDTYKEFIERHAKAMLKPE"
     gene            complement(1150..1620)
                     /locus_tag="VNU48_05290"
     CDS             complement(1150..1620)
                     /locus_tag="VNU48_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HWI10724.1"
                     /translation="MNRLAAILPASNVLVDVDATSKKRAFEHAGLVFENQHSIARATV
                     TDNLFARERLGSTGLGHGVAIPHGRIKGLKNPLAAVLRVQQPIPFDAPDDEPVGLLIF
                     LLVPEAATQRHLEILSEIAEMLSDRELRERLKTEPDAATVHQLIANWEPLKSVA"
     gene            complement(1855..2196)
                     /gene="raiA"
                     /locus_tag="VNU48_05295"
     CDS             complement(1855..2196)
                     /gene="raiA"
                     /locus_tag="VNU48_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HWI10725.1"
                     /translation="MNLTISGHHLDVTPALREYVLTKLDRVTRHFDQVVDINVLLSVE
                     KLKEKERRQKAEVTLHVKGKDIFIEQCHEDLYAAIDQLMDKLDRQVCRHKDKVQDHHH
                     ASPKRIDAAPV"
     gene            2597..3580
                     /gene="corA"
                     /locus_tag="VNU48_05300"
     CDS             2597..3580
                     /gene="corA"
                     /locus_tag="VNU48_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HWI10726.1"
                     /translation="MLNVFTLASGRLAQQEIESLAALAQALTRVQPIWVDLDAPTEDE
                     KSWIAQHFGVTIPADVVDDDLEESARFYEEDNGELHIRSDFLIDADIDDGGPRNVRVA
                     FILFRNVLFSIHAEELPVFRLLRLRARRIPALIEDAKDVLLKLYDADAEYSADALEGI
                     YDSLEKVSARVLTQDVNDQAAGEALNAIAREEDLNGRIRRNVMDTRRAVSFMMRSRML
                     NAEQFEEARQILRDIDSLDSHSTFLFDKINFLMNATVGFININQNKIIKIFSVASVAL
                     LPPTLIASIYGMNFKAMPELDWGWGYPFALALMLASVAAPFVFFRRKGWLR"
     gene            4003..4875
                     /locus_tag="VNU48_05305"
     CDS             4003..4875
                     /locus_tag="VNU48_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWI10727.1"
                     /translation="MIYPELFKQLEAVRWNMDKDIPWDHFDASKLTEEQAQTIKMNAI
                     TEWAALPATEMFLRDNKGDADFCGFMSVWFFEEQKHSLVLMEYLRRFRPELVPTEAEL
                     EAVRFEFDPAPALETLMLHFCGEIRLNHWYRRAAEWHTEPVIQAIYETLARDEARHGG
                     AYLRYMKRALQNFGDEARAAFAKVGVLMASARRTAQALHPTNLHVNAKLFPRDTVQSR
                     LPNPEWLEQWLDKQIQFDAVWETKVVERILHNMSLLMERSFASVQELNRFRKEVTAAV
                     AAAAKATAAGLQPA"
     gene            4889..5365
                     /gene="rfaE2"
                     /locus_tag="VNU48_05310"
     CDS             4889..5365
                     /gene="rfaE2"
                     /locus_tag="VNU48_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HWI10728.1"
                     /translation="MSPSFLDKLCPRAELAQRVAALARPVVFTNGVFDILHRGHVSYL
                     AQARALGGSLVLGLNSDASARRLGKGPDRPLNPELDRACVLAALESVSLVTLFDEATP
                     VELMKLVRPQLYVKGGDYDIETLAETQWVRSWGGEARALAFVDGYSTTALVARIRA"
     gene            complement(5381..5920)
                     /locus_tag="VNU48_05315"
     CDS             complement(5381..5920)
                     /locus_tag="VNU48_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10729.1"
                     /translation="MIESAGLRIVLRAALCAAVSWGVWRLLGLAPMVATAPLWGVALA
                     KPLVELASDLRHRMRHHVWKPLEGRHFVFRGLPLQVLEDADHRRWVRAADVRRIVGHT
                     ASNNALALTYPNGWRLLGQPAEPHFSDEALIAHLTKESAPEALRFRHWAEREIAFPAR
                     RLRERLGIRTEPPDFVASD"
     gene            complement(5917..6813)
                     /locus_tag="VNU48_05320"
     CDS             complement(5917..6813)
                     /locus_tag="VNU48_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWI10730.1"
                     /translation="MTTERKSHLDTLAVTSLVGCCFLWGLNQVAAKAAMPEVPPLWQA
                     ASRSLGGTVLVALWARWRGIDLFTADATLRGGLLAGALFAAEFVCIFVGLQFTTASRM
                     AVFIYISPFVVALGMPFIAHAERLSRAQLAGLLLAFSGVAWAFAEGFTKPAAGPHQWL
                     GDGLGVLAGVLWGATTLAIRGTKLAAASAEKTLLYQLAISGALLLLLAVAQGAPLPAR
                     LSTLAWASMGFQIVIVTAASYLVWFWLIRRYPATRLASFTLLTPVFGLLLGALLLGEP
                     ITVRLVVALAAVATGIVLVNRR"
BASE COUNT         1107 a         2347 c         2384 g         1002 t
ORIGIN      
        1 cgccgggccg ctcccaagcc ggcccggacc ccctcggggg gtggcccggt aaaccgggcc
       61 gggggctcca ctcactctgg cttgagcatc gccttggcgt ggcgctcgat gaactctttg
      121 taggtgtcga tcccgcgcag ctgcaggatc gtgttgcgca ccgcggcctc gaccagcacc
      181 gcgaggttgc gtccggcatc gaccgcgatc acggtcttgc gcaccggcac gtccagaatc
      241 tcttcgttca gcggctcgta gggcaggcgc tcgaactcgc ggtccatcgt ctccttgcgc
      301 accaggtgga cgatcagctt caggcgcatc ttgcggcgca ccgcggtctc gccgaagatc
      361 gccttgatgt cgagcaggcc gatgccgcgc acctcgagca ggttcagcag caggtcgggg
      421 cagcggccct ccaggccggt ctgcgagacg cggtacaggt ccacggcgtc gtcggcgacg
      481 aggccgtggc cgcgcgagat cagctccagc ccgagctcgc tcttgcccag ccccgactcg
      541 ccggtgatca gcacgccgac gccgaggatg tccatgaaca cgccgtggcg cgtggtgcgc
      601 tcggcgaagt gcaggccgag gtaggcccgc accacgtcga tcacgtggcc ggccgattca
      661 ccggtggcga acagcgggat gtcggcgcgg tcgcacagcg cgaccagttt gtcgggcggc
      721 gtctcgccat cggcgacgat cagcaccggc ggctcgagcg tgacgatgcg cgagatgcgg
      781 cgctcggcca cttcgggcgc gacgctcagg taggccacct cgcgccggcc gacgatctgc
      841 acgcggtagg ggtggatgta gttcaggtag ccgaccaggt cggcggccga ctgggcactg
      901 cgcaccgcgg cctcgtcgaa gcggcgctcc gggtgggcgt ggccggcgat ccactcccag
      961 tgcaggatct cgcgatgctc ctcgaacagc gcctccgcgc tgatgacgct gggcttcatg
     1021 ggacccccgg cccgatgggt acaccgggcc accccccgag ggggtgcggg ccggcttggc
     1081 tgggccacga aggggcccct gcgtggccct cgcccggcgc tcggcccgcc gcgccgttgg
     1141 ccacgcgcat caggccaccg acttcagcgg ttcccagttg gcgatcagct ggtgcaccgt
     1201 ggcggcgtcg ggttcggtct tcaggcgttc gcgcagttcg cggtccgaca gcatctcggc
     1261 gatctcggac aggatctcga ggtgccgctg cgtcgccgcc tcgggcacga gcaggaagat
     1321 cagcaggccg accggctcgt cgtcgggcgc gtcgaacggg atcggctgct gcacgcgcag
     1381 caccgcggcc agcgggttct tcaggccctt gatgcggcca tgcgggatcg ccacgccgtg
     1441 ccccaggccg gtcgaaccca ggcgctcgcg cgcgaacagg ttgtcggtca ccgtcgcgcg
     1501 ggcgatcgag tgctggttct cgaacaccag cccggcgtgc tcgaaagcgc gcttcttgct
     1561 ggtggcgtcg acatccacca gaacgttgct ggcaggcagg atggcagcga ggcggttcat
     1621 cggcggcaca cacgcatggc ttgaggtttc ggctccggtc gccctggcga tcgcggaggt
     1681 ggcgggttgc gaattatggg acgcttgccg gcaccggacg caagccccgt gccgcaggtc
     1741 ggcaagattg tgcattgttg ctgcatggca gcaacgaagc agcctccatt gcccctggcc
     1801 ggttcaacga aaaggcggcc cgcaggccgc cttcgctgcc agccgctcgg cccttcagac
     1861 cggcgccgcg tcgatgcgct tcggcgatgc gtggtggtga tcctgaacct tgtccttgtg
     1921 gcggcagacc tggcgatcga gcttgtccat cagctggtcg atcgctgcgt agagatcttc
     1981 gtgacactgc tcgatgaaga tgtccttgcc cttcacgtgc agcgtgacct cggccttctg
     2041 gcggcgctcc ttctccttca gcttctcgac cgacagcagc acgttgatgt cgacgacctg
     2101 gtcgaagtgg cgcgtgaccc gatccagctt ggtgagtacg tactccctga gggctggtgt
     2161 gacatcgaga tgatggccgc tgatggtcag attcatgagg gtcctttcgc aggtggggga
     2221 aggcgggccg ggggacgcgg ctcaggcttg gaagccggtc cggcaagacc gtcgaaccca
     2281 gtgtgcccag cttcccggcc catgacaagc ggatgacacc gatcctcaca aggtccgcgt
     2341 ttcgcgcgtg caaacccgtc ggcgcgccgg cgccaaacgg cccgatacgg gtgcgataat
     2401 ccaaggctgc agcttggaga tttgcttgga cctgcctcac cctcggtgac cgtccgctcc
     2461 agcgcgtgcc ggcgccggac gaggttgcag caagcgccgg ggcgaggcgg tcgccaccct
     2521 caccccggat accctctctg cgccacgccg cagcagcgcc gtcgccgcgc tgccagagag
     2581 ttgcgttgga gccctcatgc tcaatgtgtt cacgctggcc agcggacggc tcgcccaaca
     2641 ggaaatcgaa agcctggccg cgctggcaca ggcgctgacg cgcgtccagc cgatctgggt
     2701 cgatctcgac gcgccgaccg aggacgagaa aagctggatc gcccagcact tcggcgtcac
     2761 gatcccggcc gatgtggtcg acgacgacct tgaggaatcg gcgcgcttct acgaggagga
     2821 caacggcgag ctgcacatcc gctccgactt cctcatcgac gccgacatcg acgatggcgg
     2881 cccgcgcaac gtgcgcgtcg cgttcatcct gtttcgcaac gtgctgttct cgatccatgc
     2941 cgaggagttg ccggtgttcc gcctgctgcg cctgcgcgcg cggcgcattc cggcactgat
     3001 cgaggacgcg aaggacgtgc tgctcaagct ctacgacgcc gacgccgagt acagcgccga
     3061 cgcgctcgaa ggcatctacg acagtctcga gaaggtcagc gcgcgcgtgc tcacgcagga
     3121 cgtcaacgac caggccgccg gcgaagcgct gaacgcgatc gcgcgtgagg aagacctgaa
     3181 cggccgcatc cgccgcaacg tgatggacac gcgccgcgcg gtcagcttca tgatgcgttc
     3241 gcgcatgctc aatgccgagc agttcgagga ggcgcgccag atcctgcgcg acatcgactc
     3301 gctcgacagc cacagcacct tcctgttcga caagatcaac ttcctgatga acgcgacggt
     3361 cggcttcatc aacatcaacc agaacaagat catcaagatc ttctcggtcg ccagcgtcgc
     3421 gctgctgccg ccgacgctga tcgcgagcat ctacggcatg aacttcaagg ccatgcccga
     3481 actggactgg ggctggggct acccgttcgc gctggcgctg atgctggcgt cggtggcggc
     3541 gccgttcgtc ttcttccggc gcaagggctg gttgagatga cgccggcgct cggtcgcgtc
     3601 gcagagtgcg accggaggcc gcgatgcggc ctccggcgtg gagaagagtg ctgcgaagcg
     3661 cccccgaggg aatcagggag ggaggaggga ggagatcaac cgcccgggga ttgcgaccgg
     3721 accgaaccgg cggcttcgca acaaaaaacg gggtgacagg caccgcccgc agtgcgcggc
     3781 atggcgggtc cgcagcattg gaaggcagac cccgccccaa gttccctggc gacgcacgta
     3841 aagaaatgcg accgtttggt cagcgcgtgc caaggcatgc ggatcgtggc ccgaggtcgg
     3901 tgggcggatg gggttgggcg ccgcccgcgt ccggcgcccg cccgcccgca catcggtcac
     3961 acggcgcaga ctagaatccc gcgcactacg aggccccacg cgatgattta ccccgaactt
     4021 ttcaagcagc tcgaagcggt gcgctggaac atggacaagg acattccttg ggaccatttc
     4081 gacgccagca agctcaccga agagcaggcc cagacgatca agatgaacgc gatcaccgag
     4141 tgggcggcgc tgccggccac cgagatgttc ctgcgcgaca acaagggcga tgccgacttc
     4201 tgcggcttca tgagcgtgtg gttcttcgag gagcagaagc attcgctggt gctgatggag
     4261 tacctgcggc gcttccggcc cgaactggtg cccaccgagg ccgagctgga ggcggtgcgc
     4321 ttcgagttcg acccggcgcc cgcgctcgag acgctgatgc tgcacttctg cggcgagatc
     4381 cgcctgaacc actggtaccg gcgcgccgcc gagtggcaca ccgagccggt gatccaggcg
     4441 atctacgaga cgctggcacg cgacgaggcg cgccacggcg gcgcgtacct gcgctacatg
     4501 aagcgcgcgc tgcagaactt cggcgacgag gcgcgcgcgg cgttcgcgaa ggtcggcgtg
     4561 ctgatggcga gcgcgcgacg caccgcgcag gcgctgcacc cgacgaacct gcacgtcaat
     4621 gccaagctgt tcccgcgcga cacggtgcag agccggctgc cgaacccgga gtggctcgag
     4681 cagtggctgg acaagcagat ccagttcgat gcggtctggg agaccaaggt ggtcgagcgc
     4741 atcctgcaca acatgagctt gctgatggag cgcagcttcg ccagcgtcca ggagctgaac
     4801 cgcttccgca aggaggtgac ggctgccgtc gcggccgcgg ccaaggctac cgctgcgggg
     4861 ttgcagccgg cctgagccgc gtcggcgcgt gagcccgagc ttcctcgaca aactctgccc
     4921 gcgcgccgag ctggcgcagc gggtggccgc gctggcgcgg ccggtggtgt tcacgaacgg
     4981 cgtgttcgac atcctgcacc gcggccatgt cagctacctg gcgcaggcgc gcgcgctcgg
     5041 cggcagcctg gtgctcgggc tgaacagcga cgcgtcggcg cgccggctgg gcaaggggcc
     5101 ggaccgcccg ctgaaccccg agctcgaccg tgcctgcgtg ctcgcggcgc tcgagagcgt
     5161 cagcctcgtg accctgttcg acgaggccac gccggtcgag ctgatgaagc tggtgcgccc
     5221 gcagctgtac gtgaagggcg gcgactacga catcgagacg ctggccgaga cgcagtgggt
     5281 gcgcagctgg ggcggcgagg cgcgcgcgct ggcgttcgtc gacggctact cgacgaccgc
     5341 gctggtcgcg cgcatccgcg cctgatccaa ctgaccggcc tcagtcgctc gcgacgaagt
     5401 ccggcggctc ggtgcggatg cccagccgct cgcgcaggcg tcgcgccggg aacgcgatct
     5461 cgcgctcggc ccagtgccgg aagcgcagtg cctcgggcgc gctctccttg gtcaggtgcg
     5521 cgatcagcgc ctcgtcgctg aagtgcggtt cggccggctg gcccagcagg cgccagccgt
     5581 tcgggtaggt cagcgcgagc gcgttgttcg acgcggtgtg gccgacgatg cggcgcacgt
     5641 cggcggcgcg cacccagcgg cggtggtcgg cgtcctcgag cacctgcagc ggcaggccgc
     5701 gaaacacgaa gtgccggcct tcgagcggct tccacacgtg gtggcgcatc cgatgacgca
     5761 ggtcgctcgc gagttcgacc agcggcttgg ccagcgccac gccccacagc ggcgcggtgg
     5821 ccaccatcgg cgccaggccc agcaggcgcc agacgcccca cgacaccgcc gcgcacagcg
     5881 ccgcgcgcaa cacgatgcgc agcccggccg actcgatcat cggcggttca ccagcacgat
     5941 gccggtcgcg accgcggcca gcgcgacgac cagccgcacg gtgatcggct cgcccagcag
     6001 cagcgcgccg agcagcagcc cgaacaccgg cgtcagcagc gtgaacgagg ccaggcgcgt
     6061 cgccgggtag cggcggatca gccagaacca gaccaggtag ctcgccgccg tgacgatcac
     6121 gatctggaag cccatcgagg cccaggccaa ggtcgacagc cgcgccggca gcggcgcccc
     6181 ttgcgccacc gcgagcagca gcaacagcgc gcccgagatc gcgagctggt acagcagcgt
     6241 cttctcggcc gacgcggccg cgagcttcgt gccgcggatc gccaaggtgg tcgcgcccca
     6301 gagcacgccg gcgagcacgc ccaggccgtc gccgagccat tggtgcggcc cggcggcggg
     6361 cttcgtgaag ccttcggcga aggcccaggc gacgccgctg aaggccagca gcaggccggc
     6421 cagctgtgcg cgcgacagcc gctcggcatg cgcgatgaac ggcatcccca gcgcgaccac
     6481 gaacggcgag atgtagatga acaccgccat gcgcgacgcg gtcgtgaact gcagcccgac
     6541 gaagatgcag acgaactcgg ccgcgaacag cgcgccggcg agcaggccgc cgcgcagggt
     6601 tgcatcggcc gtgaacaggt cgatgccgcg ccagcgcgcc cacagcgcga ccagcaccgt
     6661 gccgccgagc gagcgcgagg cggcctgcca cagcggcggc acctcgggca tcgccgcctt
     6721 cgccgcgacc tgattcaggc cccacagaaa gcagcagccc accagcgagg ttaccgccag
     6781 cgtgtcgaga tggctcttgc gttcagtcgt catgctcgtg cgcgcgcgcg gccgggtgcg
//
LOCUS       DATMJB010000240         3711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_307260, whole genome shotgun sequence.
ACCESSION   DATMJB010000240 DATMJB010000000
VERSION     DATMJB010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307260"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..>3711
                     /locus_tag="VNU48_05325"
     CDS             9..>3711
                     /locus_tag="VNU48_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10731.1"
                     /translation="MNISDTQPDTSRGSVRGDAASVSSPEMHDYNEADFDTRPSVDRP
                     MFEPIEDSRPARRAANDDRETIGQILQAIQKGRPARNVYTLATLFAGVWLVGCGLLTV
                     SFLPSLQAAIGQGAGGTLVLGGLAALFFAPVLLFYFLASLSWRGQELRMIAQSMAQVA
                     IRFSEPEGAAADSMVTVGQAIRREVAAMGDGVERAIARAGELETLVANEVSALERAYS
                     DNEVRIRALLQDIAHQRDNLVGQAEQVRSAISGVQIDLRHDIALISDAIASRVDEVAK
                     SITGALEERGAHITGALSNAGDNMILALGERGGDLLDRLEEASAETTRAVLDASERLT
                     TSLNFKTGHVHDEFVDLADRVHEMLNERIDRITSEFEQRSSTIVDGISDRTEQVHDSL
                     KNSSDSLLLELELRSGDLVSRIDDAGNRLATQILTSGDKASDALDVTVNSLVAKVVSQ
                     TETAHDSLSLQMSAFDELVKNQGGELAEKFSRDSGTLGALITRHISEFDRTVKTFGGE
                     IVERMGQRTQDIAETLKTYVDTFDNRLTSNSGEITASLDQRLVQFETTLESRVAHLDS
                     SLDGKIRSFDESVDGRLKSLEHTFDSRAKSVTETIDNRLGALSTSLTDGAMQAIHSID
                     SRLTHLTSSLTDGTAQAIHSIDSRLSNLTTSLSEGTAKAIAAVDQRISSVTETIDGRS
                     TQLTDTVTARFLEIHQGIETRVGAVATDIDMRVAQFEDLLGSRVEAVAGRIESSGRQA
                     SDDLMARAELLSSGIKSHVEDAERSLTNLVVNTSETIQTGARAAQQSLLTVSSDVGAQ
                     LKLTSTEVERTLTAVGSGAAESILTSARNAQNALVNASSEAATQIKSLSADVEQTLSA
                     AGAATATAIVAGAREAQTTLVTASADATSQVKSLAGDVERTLSAAGNATAASILSGAR
                     EAQTTLVTASADAASQVKSLAADVERSLSIVGSSTAEAITSGARQAQSTLLTTSEDVA
                     GQVRSLVADVERSLSIAGASTAESISASARDAQDTLVTASSNAADHVKALSADVQRSL
                     SMAGASTAEAITTGARDAQNTLVAASSAAAEHVMSLSSDIQRSLSITGTATAETITSG
                     AREAHNLLATASSDAANHVKSLAIDVERTLTAVGADTAASILASAREAQTSLTATHAD
                     AASQIKAISTDIERSLGTVTANTTDNIKVTAQAAQSALVAASNEVSAKVKTSSADIER
                     SILAASGSFGSAMTGKTDEIVTYVQQQTDR"
BASE COUNT          703 a         1335 c         1118 g          555 t
ORIGIN      
        1 aggaagcttt gaacatcagc gacacgcagc cagacaccag ccgaggttca gtccgcggcg
       61 atgctgcttc ggtcagttcg cccgagatgc acgattacaa tgaggccgac ttcgacaccc
      121 ggccgagcgt cgatcgcccg atgttcgaac cgatcgaaga ctcgcgcccc gcgcgccgcg
      181 ccgccaatga tgaccgcgaa accatcggcc agatcctgca ggccattcag aagggccggc
      241 ccgcccgcaa cgtctacacg ctcgccacgt tgtttgccgg tgtctggctt gtcggctgcg
      301 gcctcctgac cgtcagcttt ctgccctcat tgcaggccgc gatcggccag ggcgccggcg
      361 gcacactggt actgggcggt ctcgcggcgc tgttctttgc gccggtgctg ctgttctact
      421 tcctcgccag cctctcctgg cgcggccagg aattgcgcat gatcgcgcag tcgatggccc
      481 aggtcgcgat ccgcttctcg gagcctgaag gggctgccgc cgactcgatg gtaaccgtcg
      541 gtcaggccat ccgccgcgag gtcgccgcca tgggcgacgg cgtcgaacgc gcgattgcgc
      601 gcgccggcga actcgaaacc ctcgtcgcca acgaagtctc cgcgctcgag cgcgcctaca
      661 gcgacaacga ggtgcgtatc cgcgcgttgc tgcaggatat cgcccatcag cgcgacaatc
      721 tggtcggcca ggccgagcag gttcgcagcg ccatctccgg cgtgcagatc gacctgcgcc
      781 acgacatcgc gctgatctcg gacgcgatcg cctcgcgcgt cgacgaagtc gccaagagca
      841 tcaccggcgc gctggaagag cgcggcgccc acatcaccgg cgcgctgagc aatgccggcg
      901 acaacatgat cctcgcactc ggcgagcgcg gcggcgacct gctcgatcgc ctcgaggaag
      961 ccagcgccga gaccacccgc gccgtgctcg acgccagcga acgcctcacc accagcctca
     1021 acttcaagac cggccacgtc catgacgagt tcgtcgatct cgccgaccgc gtccatgaga
     1081 tgctgaacga gcgcatcgat cgcatcacca gcgaattcga gcagcgttcc tccaccatcg
     1141 tcgacggcat ttccgatcgt accgaacagg tccacgactc gctgaagaat tccagcgact
     1201 cgctgctgct cgaactcgaa ctgcgcagcg gcgatctcgt cagcaggatc gacgacgccg
     1261 gcaaccgtct cgccacccag atcctcacca gcggcgacaa ggccagcgac gcgctcgatg
     1321 tcaccgtgaa ctcgctggtg gcgaaggtcg tgagccagac cgaaaccgcg catgacagcc
     1381 tcagcctgca gatgagcgcg ttcgacgaac tggtgaagaa ccagggcggc gaactcgccg
     1441 agaaattctc gcgggacagc ggcacgctgg gcgcattgat cacccgccac atctccgaat
     1501 tcgatcgcac cgtcaaaacc ttcggcggcg agatcgtaga gcgcatgggc cagcgcaccc
     1561 aggatatcgc cgagacgctc aagacgtacg tcgacacctt cgataatcgc ttgacctcga
     1621 acagcggcga gatcaccgcc tcgctcgacc agcggctggt gcagttcgag accacgctgg
     1681 aaagccgggt ggcccatctc gatagctcgc tcgacggcaa gatcaggtcg ttcgacgaat
     1741 ccgtcgacgg ccggctcaag tcgctcgaac ataccttcga ttcacgggcg aagtcggtca
     1801 ccgaaaccat cgacaaccgg ctcggcgcat tgtcgacgtc gctgaccgat ggcgctatgc
     1861 aggcgatcca ttcgatcgac tcgcgcctca cccacctcac ctcgtcgctg accgacggca
     1921 ccgcgcaggc gatccactcg atcgactcgc ggctgtccaa cctcacgacg tcgctgagcg
     1981 agggcaccgc gaaagcgatc gcggcggtcg accaacgcat ctccagcgtc accgagacca
     2041 tcgacggccg cagcacgcag ctcaccgaca ccgtcacggc gcggttcctg gaaatccatc
     2101 agggcatcga aacccgggtc ggcgcggttg cgaccgatat cgacatgcgc gtcgcccagt
     2161 tcgaagatct cctgggttcg cgcgtcgaag ccgtggccgg ccgcatcgaa agcagcggac
     2221 gccaggccag cgacgacctg atggcacgcg ccgaactgct gtcgtcgggc atcaagtccc
     2281 atgtcgagga cgcggaacgc tcgctgacca acctcgtggt caacaccagc gaaaccatcc
     2341 agaccggggc gcgcgcagcg cagcagtcgc tgctgacggt ttcctccgac gtgggcgcac
     2401 aactcaagct gacctcgacc gaagtcgagc gcaccctaac cgccgtcggc tccggcgccg
     2461 cggaatcgat cctgaccagc gcccgcaatg cccagaacgc cctggtcaat gcctcctccg
     2521 aggccgccac ccagatcaag tcgctgtccg ccgacgtcga acagacgctg tcggccgccg
     2581 gcgccgccac cgccaccgcg atagtcgcgg gcgctcgcga agcccagacc acgctggtca
     2641 cggcttcggc ggacgcgacc agccaggtca aatcgctggc cggcgacgtc gaacgcacgc
     2701 tttcagccgc gggtaacgcc acggccgcct ccatcctgtc cggtgcgcgc gaagcccaga
     2761 ccacgctggt cacggcttcc gccgatgcgg cgagccaggt caagtcgctg gcggccgacg
     2821 tcgaacgctc gctgtccatc gtcggatctt ccaccgccga ggccatcacg tcgggcgcac
     2881 gtcaggcgca gtcgacgttg ctgacgacgt ccgaagacgt cgccggccag gtcaggtcgc
     2941 tggtggccga tgtcgagcgt tcgctctcga tcgccggggc ctcgactgcc gagtcgatct
     3001 ccgccagcgc gcgcgatgca caggatacgc tggtcaccgc gtcgtcgaat gccgccgacc
     3061 acgtcaaggc gctctcggcc gatgtgcagc gttcgctgtc gatggcaggc gcatcgaccg
     3121 ccgaggcgat caccacaggt gcgcgcgatg cccagaatac ccttgtcgcc gcatcgtcgg
     3181 cggctgccga gcacgtcatg tcgctgtcat cggatatcca gcgctcgctc tcgatcacgg
     3241 gcaccgcgac cgcggaaacg atcactagcg gtgcgcgcga agcccacaac ttgctcgcca
     3301 ccgcttcatc ggatgccgcc aaccacgtca agtcgcttgc gatcgatgtg gagcgcaccc
     3361 tgacggcagt cggcgcggat accgccgctt cgatcctggc cagcgcacgt gaggcgcaga
     3421 cttcgctgac cgcaacccac gccgatgcgg caagccagat caaggcgatc tcgaccgaca
     3481 tcgagcgttc actcggcacg gtcaccgcca acaccaccga caacatcaag gtcacggcac
     3541 aggccgcgca gagtgccctg gtggccgcct cgaacgaggt cagcgccaag gtcaagacga
     3601 gctcggccga catcgagcgg tcgattctgg ccgccagcgg ctcgttcggt tcagccatga
     3661 cgggcaagac cgacgaaatc gtaacctacg tccagcagca gaccgatcgt c
//
LOCUS       DATMJB010000241         4806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_689356, whole genome shotgun sequence.
ACCESSION   DATMJB010000241 DATMJB010000000
VERSION     DATMJB010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689356"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="VNU48_05330"
     CDS             complement(<1..1385)
                     /locus_tag="VNU48_05330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWI10732.1"
                     /translation="MEATRPAALTGAATDNAQLAKAALRRLALDKLDPTPENYARAWR
                     QEAGETQPPVADGPAIATLIGRIVRGIERGSKGWTAARKKDSLQRVLDGSRSDARRLQ
                     QRLSQLVASWDSEAAPAVEACEDDPATIFEPMPTSAALLEALAPLPWGRAVNALGVGL
                     QAALPQQGEAPHEITLALATLIERIEHEGATPALIDELEALCRRTVPVLQHRHHLLDQ
                     LGSLCVELTASLTDLAENDSWAKGQCEAMQVKIDEGLSARGIRAVSELLRDTRARQGR
                     LREERERARDSLKGLINRMLSELDQLGSQTGNFHDSVGRYADVIEKADSLESLAGAVR
                     EMVEESRSVQALVQQTQARLQAEHSKATGLTQRVSELEDEMRRLSDEVSTDQLTKIAN
                     RRGLMQAFEAERARLQRDGGQLSIGLLDIDNFKRLNDDLGHTAGDEALKALATLVDHT
                     LRPSDHVARYGG"
     gene            complement(1409..3664)
                     /locus_tag="VNU48_05335"
     CDS             complement(1409..3664)
                     /locus_tag="VNU48_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWI10733.1"
                     /translation="MNARGVAVDDDLPEQPKVLLVDDDEVNLLLTSIALRERGFAITE
                     ASSGEQAIEILGDWVPDVVVLDALMPGLDGFQTCRKLRGLPGFESLPVLMLTGLDDDA
                     SITRAYEAGATDFFVKSTQWSLLAGRLRYLLRASRTRAELERSKSRLARAQDLARMGS
                     FDWRRGHGGPLFSIEGLRVFGLGPTDGLAFRPLLRMLSNDDRTGLMQVLHEVMKHSTV
                     LATDIPATLRDGRLRIVHVEAEPEFNEHGNLIGYSGIVQDVTDRRMAEDRIRHLANFD
                     VLTGLPNRRQLIWRAERALEHGRRLNHQVALLLIDLDRFKIINDTLGHGAGDELLMEV
                     ARRLRSCVRHSDQVTESSIESLGARSHRSLEAVGRLGGDEFVALLPEVADERDAERVA
                     ARILDLMREPIFVGGQECFVTASVGIALYPRDGASVADLMRNSDVAMYSVKNTTRNSS
                     SVYRPALAGQGREKLERESALHKALERGELVLHYQPKVDVRAAKMVGAEALMRWHHNG
                     VMVPPADFIPLAEETGLIVSFSEWAIREAARQARVWQDSFGFADSIAVNLPIRMFERT
                     DLVEHIHNAVTTYGVPHHAIELEITETGLMKDLQNVIPSLHRLNEIGVEISVDDFGTG
                     YSSLAYLTTLPISELKIDRSFVRDLGMTPQSSAVVTAIIALARSLGLRVIAEGVENLR
                     QMEVLHRLGCGVMQGFLFSRPQPPEDIETWLQQTILPKKAPWIGKAGEAEMAEVLRFA
                     EGRDPRLKPAV"
     gene            complement(3661..>4806)
                     /locus_tag="VNU48_05340"
     CDS             complement(3661..>4806)
                     /locus_tag="VNU48_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWI10734.1"
                     /translation="GARAVTILEVHDVNATHARISDLIESPPGFRTVPRRVRLDTAPL
                     ARALREQRPVGTPPGAVVLPVLADGRVRAAIELSAIDLAIDPQALAGLLELAGSALAA
                     RAPAAAPLAGADPASAARCANVLVIEDTPAHADTTEGLLRRVGCHATRASGMLDALAA
                     LHGRQFDLVLTDLPPGAIEACEGWRRLLRRDAFDAGPAAPARGTPMIALCAADAPGDG
                     DRFRELGFDDHLFKPIRPDQLLAMLSKHLRPHAPAEAEGVPAGAAPAVLDAAALARLG
                     DLDPNGTNHLLERVLRAFETSVARLRPQLDAARAGDDRAAIRLVAHTLKSSSASIGAL
                     QLSQLCAQIEAAIRMDTGESLLPRLDALDAALDGALQAIAALLKERG"
BASE COUNT          652 a         1718 c         1713 g          723 t
ORIGIN      
        1 ccgccgtagc gggcgacgtg gtcggacggc cgcagcgtgt ggtcgaccaa cgtggcgagc
       61 gccttcaacg cctcgtcgcc ggcggtgtgg ccgaggtcgt cgttgaggcg cttgaagttg
      121 tcgatgtcga gcaggccgat cgacagctgc ccgccgtcgc gctgcaggcg cgcgcgctcg
      181 gcttcgaacg cctgcatcag cccgcgccgg ttggcgatct tggtcagctg gtcggtcgag
      241 acctcgtccg agagccgccg catctcgtct tcgagctcgc tgacgcgttg cgtcaggccg
      301 gtcgccttgc tgtgctcggc ctgcaggcgc gcctgcgtct gctgcaccag cgcctgcacg
      361 ctgcggcttt cctcgaccat ctcgcgcacc gcgccggcca ggctctcgag cgaatcggcc
      421 ttctcgatca cgtcggcata gcggccgacg ctgtcgtgga agttgccggt ctgcgagccg
      481 agctggtcga gctcgctgag catgcgattg atcaggcctt tgagcgaatc gcgcgcgcgt
      541 tcgcgctcct cgcgcagccg gccctggcgc gcccgcgtgt cgcgcagcag ctcactgacc
      601 gcgcggatgc cgcgtgccga aaggccttcg tcgatcttga cctgcatcgc ctcgcactgg
      661 cccttggccc agctgtcgtt ctcggccaga tcggtcaggc tcgcggtcag ctcgacgcac
      721 aggctgccga gctggtcgag caggtgatgc cggtgctgca gcaccggcac ggtacgccgg
      781 cacagcgctt cgagctcgtc gatcagcgcc ggcgtggcgc cctcgtgctc gatgcgctcg
      841 atcagcgtgg ccagcgcgag cgtgatctcg tgcggcgcct cgccctgctg cggcagcgcg
      901 gcctgcaggc cgacgccgag cgcattgacg gcgcgccccc agggcagcgg cgccagcgcc
      961 tcgagcagcg cggccgaggt cggcatcggc tcgaagatcg tggccgggtc atcctcgcag
     1021 gcctcgacgg ccggggcggc ctcgctgtcc cagctcgcga cgagctgact caggcgttgc
     1081 tgcaggcgtc gcgcgtcgct gcggctgccg tcgagcacgc gctgcaggct gtctttcttg
     1141 cgcgccgcgg tccagccctt gctgccgcgc tcgatgccgc gcacgatgcg cccgatcagc
     1201 gtcgcaatgg ccggcccgtc ggccaccggc ggttgcgtct cgccggcctc ctggcgccac
     1261 gcgcgggcgt agttctcggg cgtggggtcg agcttgtcga gcgcgagccg gcgcagcgcg
     1321 gccttcgcga gctgggcgtt gtcggtggcg gcgccggtca gcgcggcggg gcgggtcgct
     1381 tccatgcgga acgccgtgcg gtgcagactc agacggccgg cttgaggcgc gggtcgcggc
     1441 cctcggcgaa acgcagcacc tcggccatct cggcctcgcc ggccttgccg atccacggcg
     1501 ccttcttcgg caggatggtc tgctgcagcc aggtctcgat gtcttccggc ggctgcggcc
     1561 ggctgaacag gaagccctgc atcaccccgc agcccaggcg gtgcagcact tccatctggc
     1621 gcaggttctc gacgccctcg gcgatcacgc gcaggccgag cgagcgcgcc agcgcgatga
     1681 tcgcggtgac gaccgccgag ctctgcggcg tcatgcccag atcgcgcacg aagctgcggt
     1741 cgatcttcag ctcgctgatc ggcagggtcg tcaggtaggc gagcgacgaa tagccggtgc
     1801 cgaagtcgtc gaccgagatc tcgacgccga tctcgttcag gcggtgcagc gacgggatca
     1861 cgttctgcag gtccttcatc agcccggtct cggtgatctc gagctcgatc gcgtggtgcg
     1921 gcacgccgta ggtcgtgacc gcgttgtgga tgtgctcgac caggtcggtg cgctcgaaca
     1981 tccggatcgg caggttcacg gcgatcgagt cggcgaagcc gaagctgtcc tgccagacgc
     2041 gcgcctgacg cgccgcctcg cggatcgccc actcgctgaa cgacacgatc agcccggtct
     2101 cctcggccag cgggatgaag tcggccggcg gcaccatcac gccgttgtga tgccagcgca
     2161 tcagcgcctc ggcgccgacc atcttcgcgg cgcgcacatc gaccttgggc tggtagtgca
     2221 gcaccagctc gccgcgttcg agtgccttgt gcagcgcgct ctcgcgttcg agcttctcgc
     2281 ggccctggcc ggccagcgcg ggccgataga ccgacgagga attgcgcgtc gtgttcttca
     2341 ccgaatacat cgcgacgtcg gagttgcgca tcaggtcggc caccgaggcg ccgtcgcgcg
     2401 ggtacagcgc aatgccgacg ctggcggtca cgaagcactc ctggccgccg acgaagatcg
     2461 gctcgcgcat caggtcgagg atgcgcgcgg cgacgcgctc ggcatcgcgc tcgtcggcca
     2521 cctcgggcaa cagcgcgacg aactcgtcgc cgcccaggcg gccgaccgcc tcgagcgagc
     2581 ggtgcgagcg cgcgcccagc gattcgatcg agctctcggt gacctggtcg gagtggcgca
     2641 cgcaggagcg caggcgccgc gcgacctcca tcagcaactc gtcgccggcg ccgtggccca
     2701 gcgtgtcgtt gatgatcttg aagcggtcga ggtcgatcag cagcagcgcg acctggtggt
     2761 tcaggcgccg gccgtgctcg agcgcgcgct cggcgcgcca gatcagctgg cgccggttcg
     2821 gcaggccggt cagcacgtcg aagttcgcga ggtggcggat gcggtcttcg gccatgcgcc
     2881 gatcggtcac gtcctggacg atgccgctgt agccgatcag gttgccgtgc tcgttgaact
     2941 cgggctcggc ttcgacgtgg acgatgcgca ggcggccgtc gcgcaaggtc gcggggatgt
     3001 cggtggcgag cacggtgctg tgcttcatga cctcgtgcag cacctgcatc aggccggtgc
     3061 ggtcgtcgtt ggacagcatg cgcagcagcg gccggaaggc caggccgtcg gtcggcccga
     3121 ggccgaacac gcgcaggccc tcgatcgaga acagcggccc gccgtggcca cgccgccagt
     3181 cgaagctgcc catgcgcgcc agatcctgcg cgcgcgccag ccgcgacttg ctgcgctcga
     3241 gctcggcgcg cgtgcgcgac gcgcgcagca ggtagcgcag ccgcccggcg agcaggctcc
     3301 actgggtgga cttgacgaag aagtcggtcg cgccggcctc gtaggcacgc gtgatcgagg
     3361 catcgtcgtc caggccggtc agcatcagca ccggcagcga ttcgaaaccg ggcagcccac
     3421 gcagtttgcg gcaggtctgg aaaccgtcca ggccgggcat cagcgcgtcg agcacgacca
     3481 cgtccggcac ccagtcgccc aggatctcga tcgcctgctc gccgctgctc gcctcggtga
     3541 tcgcgaagcc gcgctcgcgc agcgcgatcg aggtcagcag cagattgacc tcgtcgtcgt
     3601 cgaccagcag caccttcggc tgctcgggca gatcgtcgtc gaccgccacg ccgcgcgcgt
     3661 tcacccgcgc tccttcagca aggccgcgat ggcctgcagc gcaccgtcta gcgcagcgtc
     3721 tagcgcatcg agccggggca gcagactctc gccggtgtcc atgcggatcg ccgcctcgat
     3781 ctgcgcgcac agctgcgaca gctgcagcgc gccgatgctc gccgacgagg acttgagcgt
     3841 gtgcgccacc aggcggatcg cggcgcggtc gtcaccggcg cgggccgcat cgagttgcgg
     3901 gcgcagccgc gccaccgagg tctcgaacgc gcgcagcacg cgctcgagca ggtggttcgt
     3961 tccgttcgga tcgaggtcgc ccaggcgcgc gagcgccgcg gcgtcgagca ccgccggcgc
     4021 ggcgcccgcg ggcacgccct cggcctcggc cggcgcatgc gggcgcaggt gcttgctcag
     4081 catggcgagc agctggtcgg ggcggattgg cttgaaaaga tggtcatcga aacccagttc
     4141 gcgaaaacgg tctccatcgc cgggcgcatc ggcggcacac agcgcaatca tcggtgtgcc
     4201 gcgcgccggc gccgcggggc ccgcatcgaa agcgtcgcgg cgcagcagcc gccgccaccc
     4261 ttcgcacgcc tcgatcgccc ccggcggcag gtcggtcagg accagatcga attgtctccc
     4321 atgcagcgcg gccagagcat ccagcatgcc agaggcgcgt gtcgcgtgac atcccacacg
     4381 tcgcaacagc ccctcggtcg tgtcggcgtg ggcgggagtg tcctcaatga ccaacacatt
     4441 cgcgcaccgc gctgcgctgg ccgggtccgc gccggccagc ggcgcggcgg ccggcgcccg
     4501 ggccgccagg gccgaccccg ccagttccag caggccggcc agcgcctggg ggtcgatcgc
     4561 cagatcgatc gccgacagct cgatcgccgc gcgcacgcgg ccatcggcca gcaccggcag
     4621 caccaccgcg ccggggggcg tgcccaccgg gcgctgctcg cgcagcgcgc gggccagcgg
     4681 cgccgtatcg aggcgcaccc gccgcggcac ggtgcgaaag ccgggcgggg attcgatcag
     4741 atcgctgatg cgcgcgtggg tcgcgttcac gtcgtggacc tcgaggatcg tgaccgcgcg
     4801 cgcacc
//
LOCUS       DATMJB010000242         2760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_298582, whole genome shotgun sequence.
ACCESSION   DATMJB010000242 DATMJB010000000
VERSION     DATMJB010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298582"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /gene="dbpA"
                     /locus_tag="VNU48_05345"
     CDS             complement(<1..1177)
                     /gene="dbpA"
                     /locus_tag="VNU48_05345"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HWI10735.1"
                     /translation="MTRSEPSHATGSFADLPLSPAQLANLRTLGYLAMTPVQAASLPL
                     ALAGDDLIVQAQTGSGKTAAFALALLAKLRLEGQSQPFAVQALVLCPTRELADQVTTE
                     IRRLARSEDNVKTLTLCGGVPLRNQQASLEHGAHVVVGTPGRLIDHLERGALQLSALT
                     TLVLDEADRMLDMGFFDDIAKIAKQCPKQRQTLLFSATYPDGIAQLAQQFMRAPKTVK
                     VRSQVDAAQIEQRFYEVRDDERLHAVSLLLQHFRPVRTLAFCNTKQQCRDLADVLVAQ
                     GFAALALHGELEQRERDQVLVQFANRSCSVLVATDVAARGLDIAQLEAVINVDTTPDP
                     QLHIHRIGRTGRADATGLALSLASLDEMGSVGKIELVQGREVTWHPLAELTQAPGGRL
                     "
     gene            1343..1537
                     /locus_tag="VNU48_05350"
     CDS             1343..1537
                     /locus_tag="VNU48_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10736.1"
                     /translation="MNAEYEIRFQSLFHEGRALCFPCDEAGQVAMDALSDRARDNYFY
                     ARAVVGREYAYPSVQPAIAH"
     gene            1742..2185
                     /locus_tag="VNU48_05355"
     CDS             1742..2185
                     /locus_tag="VNU48_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWI10737.1"
                     /translation="MNQRILTMTTIGAVVATLALAGCDKRAQTDARDGSGSTVASAEQ
                     RARDMGNDARSSLDKAKDATENAAAKMGEKVDDAVITTAVKTELAKDPDLSALRINVD
                     TANGRVALKGKAPSNEAREHATTLAQNVKGVVSVDNQLAVEPGKM"
     gene            complement(2212..2661)
                     /locus_tag="VNU48_05360"
     CDS             complement(2212..2661)
                     /locus_tag="VNU48_05360"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWI10738.1"
                     /translation="MKNLSSAALAVLALCSTIAAAAAPSDLPATAAAEKGAVVTPSGL
                     VYRSLRDGPGASPLATDTVKVHYRGTFADGSEFDSSYRRNAPAEFPLDGVIKCWTEGV
                     QRMKVGGKAKLTCPAAIAYGERGAGGGLIPPNTALQFEIELLAIAKR"
BASE COUNT          413 a          926 c         1005 g          416 t
ORIGIN      
        1 gcagccggcc gccgggcgct tgggtgagtt ccgcgagcgg atgccaggtc acctcgcggc
       61 cctgcaccag ctcgatcttg ccgacgctgc ccatctcgtc gaggctcgcc agactcagcg
      121 ccaggccggt cgcgtcggcg cggccggtgc ggccgatgcg gtggatgtgc agctgcgggt
      181 cgggcgtggt gtcgacgttg atcaccgcct cgagctgcgc gatgtccagc ccgcgcgcgg
      241 ccacgtcggt ggccaccagc accgagcagc tgcggttcgc gaactgcacc agcacctggt
      301 cgcgctcgcg ctgctcgagt tcgccgtgca gcgcgagcgc ggcgaagccc tgcgccacca
      361 gcacgtcggc caggtcgcgg cactgctgct tggtgttgca gaacgccagc gtgcgcaccg
      421 ggcggaagtg ctgcagcagc agcgagaccg cgtgcagccg ctcgtcgtcg cgcacctcgt
      481 agaagcgctg ctcgatctgc gccgcgtcga cctgcgagcg cacttttacc gtcttcggcg
      541 cgcgcatgaa ctgctgtgcg agctgggcga taccgtcggg gtaggtcgcc gagaacagca
      601 gggtctggcg ctgcttcggg cactgtttcg cgatcttcgc gatgtcgtcg aagaagccca
      661 tgtcgagcat gcgatcggcc tcgtcgagca ccagcgtggt cagcgccgag agctgcagcg
      721 cgccgcgctc gagatggtcg atcaggcgcc ccggcgtgcc gacgaccacg tgcgcgccgt
      781 gttccaggct cgcctgctgg ttgcgcagcg gcacgccgcc gcaaagcgtg agcgtcttca
      841 cgttgtcctc gctgcgcgcg aggcggcgga tctcggtcgt gacctggtcc gcgagctcgc
      901 gcgtcgggca cagcaccagg gcctggaccg cgaacggctg cgactgccct tcgagccgca
      961 gtttcgccag cagtgcgagc gcgaacgcgg cggtcttgcc gctgccggtc tgggcctgca
     1021 cgatcaggtc gtcgccggcg agcgcgagcg gcaggctcgc ggcctgcacc ggcgtcatcg
     1081 ccaggtagcc gagcgtgcga aggttggcga gttgtgccgg cgacagcggc aggtcggcaa
     1141 acgagccggt ggcgtgagag ggttcggagc gggtcatccg cgattatcgc ggcgcggccg
     1201 caggccggcc gccgacgctg cgcagggcaa ttcgctgaca cgcatcagcg agtcgccccg
     1261 acatcttgcg cgccgggtcg tgccgatact ggcatcaggc gtcggtcgct tcgacgacgc
     1321 cggtttcagg gagtgatact cgatgaacgc cgaatacgaa atcaggttcc agtcgctgtt
     1381 ccacgaaggc cgggcgctgt gcttcccgtg cgatgaagcg ggtcaggtcg cgatggacgc
     1441 gctcagcgac cgggcccgcg acaactattt ctacgcgcgc gccgtcgtcg ggcgcgagta
     1501 cgcctacccg agcgtgcagc ctgcgatcgc gcactgagcg cggcccgtcg gcgcgatgcg
     1561 gtgcggcccg gccatgccgc agcctgtggg catcgtccga cagtcgaatc gcccggcgac
     1621 cgacggcacc gtcgtggcgt cgctcctatc gttgggtcac ctccttgcgt gccgctgcgg
     1681 cgcgcgcagg gccaacggac cgggccggtc agaccggccc ttcagacaga ggagcacttc
     1741 gatgaaccag cgaatcctga cgatgacgac gatcggtgcg gtggtcgcga cgctggccct
     1801 ggccggctgc gacaagcgcg ctcagacgga tgcgcgcgat ggctcgggca gcacggtcgc
     1861 ctcggccgag cagcgcgcgc gcgacatggg caacgacgcg cgcagcagcc tggacaaggc
     1921 caaggacgcg accgagaacg ccgccgcgaa gatgggcgag aaggtcgatg acgcggtgat
     1981 cacgaccgcg gtgaagaccg aactcgcgaa ggatcccgac ttgagcgcgc tgcgcatcaa
     2041 cgtcgacacc gccaatggcc gcgtcgcgtt gaagggcaag gcgccgagca acgaggcgcg
     2101 cgagcacgcg acgacgctgg ctcagaacgt caagggcgtg gtcagcgtcg acaaccaact
     2161 ggcggtcgag ccgggcaaga tgtaggttgc ctgcccgcgc gaagcgctgt ctcagcgctt
     2221 cgcgatcgcc agcagttcga tctcgaactg cagcgcggtg ttcggcggga tcaggccgcc
     2281 gccggcaccg cgttcgccat aggcgatcgc ggcggggcag gtcagcttcg ccttgccgcc
     2341 gaccttcatg cgttgcaccc cttcggtcca gcacttgatc acgccgtcga gcgggaattc
     2401 ggccggcgcg ttgcgtctgt acgaactgtc gaactcgctg ccgtccgcga acgtgccgcg
     2461 gtagtggacc ttgacggtgt cggtggcgag cgggctggcg ccggggccgt ccctgagcga
     2521 gcggtagacc agcccgctgg gcgtcacgac agcgcccttt tcggccgccg cggtcgcggg
     2581 cagatcgctc ggcgcagcag cagcggcgat cgtcgagcac agcgcgagca cggcaagtgc
     2641 ggcggacgag agattcttca tgatgcgtgt gagggttggg gtgtgtcgtg ggcgcccgcg
     2701 accttggcca ggcgctcgcg cagcgtcgcg atcggcatga agccgctgcc caccaggtgc
//
LOCUS       DATMJB010000243         6957 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_251155, whole genome shotgun sequence.
ACCESSION   DATMJB010000243 DATMJB010000000
VERSION     DATMJB010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251155"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..481
                     /gene="dtd"
                     /locus_tag="VNU48_05365"
     CDS             41..481
                     /gene="dtd"
                     /locus_tag="VNU48_05365"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HWI10739.1"
                     /translation="MIAIAQRVARAEVSVGGERVGAIGRGLLVFACAEPGDTEVIADK
                     LVAKLLKLRIFADAQGKMNRSLVEVQGGLLIVSQFTLAADVSAGNRPSFTGAANPALG
                     RALYERLLATARAQHPAVASGAFGADMQVELVNDGPVTIPLWMR"
     gene            complement(536..1798)
                     /locus_tag="VNU48_05370"
     CDS             complement(536..1798)
                     /locus_tag="VNU48_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI10740.1"
                     /translation="MSTHYDALEARPPAQREAALMAALPAQLAHARAHAPAFAASLAG
                     VDAAAVNSRAALARLPVIRKHELLERQRGADAPDVFGGFSALAWGAAAAEHRALRVFA
                     SPGPIYEPEGANPDYWRMARALFAAGLRAGDLVHNSFSYHFTPAGAMMESGAFALGCT
                     VFPGGTGQTEQQVQAMAALKPAAYIGTPSFLKLILDKADELGVALPSLVKASVGGEAF
                     PPSLRDWMLARSIDAYQSYGTADLGLIAYETAAREGLVVDEGVIVEIVRPGTGDVLVD
                     GEVGEVVVTTLNRGYPLIRFGTGDLSAVLPGHCATGRTNTRIKGWMGRADQTAKVRGM
                     FVHPAQVAEIARRFPEVRKARLVVHGEMANDSMELRVEAQGASDGLAERIAQALRDVT
                     KLRGEVLLCAIGALPNDGKVIEDARSHR"
     gene            complement(1795..2064)
                     /locus_tag="VNU48_05375"
     CDS             complement(1795..2064)
                     /locus_tag="VNU48_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10741.1"
                     /translation="MAHADDAALQRLQPLLRQLREIKGLREMKPGIFYLKGSAFLHFH
                     DDAGALFGDLKRSRGGFDRYAVDTPVGQRKLLDDAKRRVDRGDDE"
     gene            complement(2078..2893)
                     /locus_tag="VNU48_05380"
     CDS             complement(2078..2893)
                     /locus_tag="VNU48_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI10742.1"
                     /translation="MSASPNILLNVNGIEVIYNHVILVLKGVSLQVPEGRIVALMGGN
                     GAGKTTTLRAVSNLLQSERGAVTKGSIELRGERIEALSTSEMVNRGVIQVMEGRHCFA
                     HLTIQENLMTGAYTRRDGKAAVAATLDKVYTYFPRLKTRRASQAAYTSGGEQQMCAIG
                     RALMANPKMVLLDEPSMGLAPQIVEEVFEIVRDLNTREGVTFLLAEQNTNMALRYSDY
                     GYILENGRIVMDGAAASLRENEDVKEFYLGVGGGDRKSFKDVKSYKRRKRWLA"
     gene            complement(3000..4328)
                     /locus_tag="VNU48_05385"
     CDS             complement(3000..4328)
                     /locus_tag="VNU48_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI10743.1"
                     /translation="MMFNLKSLALAAAIAASALTGPAFAQDKVQFFPSLTGRTGPVAP
                     NATAFANGFADYMKLVNARGGINGVKTLVEECETAYATDKGVECYERLKGKHGGATMF
                     QPLSTGITFALTEKVPADKIPLITSGYGRSDSADGLTFKWNFPLIGHYWVAGDTLLQH
                     IAKKEGGWDKLKGKKIAVVYHDSPFGKELLPIIQERAATHGFELLLLPVPAPGVEQKA
                     IWLQIRQQRPDFVVMQTWGVMTPTALKEAIATGYPREKMFGTWWSGAEPDLKDVGAAA
                     KGYSAVMMQHGNEQQSPVAQEILAKVHGAGQGVGPKEEVGSVIYMRGVVGAMLAVEGV
                     RAAQDRFGKGKVMSGEQARWGYENLNLTQAKLDALGFKNVLRPISTSCTDHMGSAWAR
                     VHTWDGSKFVWASDWLQADDAIIKPMVKASAGKYATEKKLVRRTGEDCQS"
     gene            complement(4400..5464)
                     /locus_tag="VNU48_05390"
     CDS             complement(4400..5464)
                     /locus_tag="VNU48_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWI10744.1"
                     /translation="MLYRENGQFKTSYRADQQIFPIAQDRIAIAALLAFAVLVVPLVA
                     PEYLFRAILIPFLILSLAALGLNILVGYCGQISLGTGAFMAVGAYAAYNFQVRIEGMP
                     LVASILLAGLCATAVGVVFGVPSLRIKGLYLAVATLAAQFFVDWAFLRVKWFTNNSSS
                     GSVSVAGLNVFGLSIETPAQKYLFCLAFTCLFALLAKNLVRSHIGREWMAIRDMDVAA
                     AVIGIRPVYAKLTAFAVSSFIVGVSGALWGFVHLGSWEPAAFNIDRSFQLLFMVIIGG
                     LGSIMGGFFGAAFIVVLPIVLDNLPYWFGIPIDTALASHLTNMIFGALIVFFLIVEPH
                     GLAKLWSIAKEKLRLWPFPH"
     gene            complement(5556..6485)
                     /locus_tag="VNU48_05395"
     CDS             complement(5556..6485)
                     /locus_tag="VNU48_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWI10745.1"
                     /translation="MGSFLETLVGGLMTGMLYSLVALGFVLIFKASGVFNFAQGAMVL
                     FSALAMARFSEWIPKGLGFDSKLLANLLAFAVAMGVMVAMAWLIEHLCLSRLVNQEGI
                     TLLMATLGIAYFLDGFGQTIFGNDIYKIDIGLPKDPIFLFESMFQGGVLVSKEDLYAA
                     LIAAALVLLLSLFFQYTGTGRALRAVADDHQAAQSIGIPLNRIWVIVWSVAGFTALVA
                     GIIWGSKLGVQFSLSLVALKALPVVILGGLTSVPGAIVGGLIIGVGEKVSEVYLGPQI
                     GGGIEIWFGYVLVLFVLLVRPQGLFGEKIIDRV"
     gene            complement(6487..>6957)
                     /locus_tag="VNU48_05400"
     CDS             complement(6487..>6957)
                     /locus_tag="VNU48_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI10746.1"
                     /translation="RNLKMKASLLQQAFRNPFGFGTAEREEIAQRAIVERIIDFLEIQ
                     PYRKTPVGKMPYGLQKRVDLGRALAMDPQVLLLDEPMAGMNVEEKQDMSRFILDVNDE
                     FGTTIVLIEHDMGVVMDLSDRVVVLDYGKKIGDGAPDAVRANPDVISAYLGTSH"
BASE COUNT         1176 a         2358 c         2288 g         1135 t
ORIGIN      
        1 ccgacgtgga ggcgcacttc gacgacctcg ccgacacgcc atgatcgcga tcgcgcagcg
       61 cgtcgcgcgc gccgaggtca gcgtcggcgg cgagcgcgtc ggcgcgatcg gccgcggcct
      121 gctggtgttc gcctgtgccg agccgggcga caccgaggtc atcgccgaca agctcgtcgc
      181 caagctgctg aagctgcgca tcttcgccga cgcgcagggc aagatgaacc gcagcctcgt
      241 cgaggttcag ggcgggctgc tgatcgtcag ccagttcacg ctggccgccg atgtgtcggc
      301 cggcaaccgg ccgagcttca ccggcgcggc gaatccggca cttggccgtg cgctctacga
      361 gcggctgctc gcgaccgcgc gtgcgcagca cccggcggtg gcgagcggcg cattcggcgc
      421 cgacatgcag gtcgaactcg tcaacgacgg gccggtgacg atcccgctgt ggatgcgctg
      481 atcggtcggc gcacccgccg gcggccttga cgacatcccg atgcattcgc aggcctcagc
      541 ggtggctgcg cgcgtcctcg atcaccttgc cgtcgttcgg cagcgcgccg atcgcgcaca
      601 acagcacctc gccgcgcagc ttggtcacgt cgcgcagcgc ctgcgcgatg cgctcggcca
      661 ggccgtcgga ggcgccctgc gcctcgacgc gcagctccat cgagtcgttc gccatctcgc
      721 cgtgcaccac caggcgtgcc ttgcgcacct cgggaaatcg gcgcgcgatc tcggccacct
      781 gcgccgggtg cacgaacatg ccgcgcacct tcgcggtctg gtcggcgcga cccatccagc
      841 ccttgatgcg cgtgttggtg cggccggtcg cgcagtggcc cggcagcacc gccgacaggt
      901 cgccggtgcc gaaacggatc agcgggtagc cgcggttcaa cgtcgtcacg acgacctcgc
      961 cgacctcgcc gtccacgagc acgtcgccgg tgccggggcg cacgatctcg acgatcacgc
     1021 cttcgtcgac gaccaggccc tcgcgcgccg cagtttcgta ggcgatcagg ccgaggtcgg
     1081 cggtgccgta gctctggtag gcatcgatgc tgcgcgccag catccagtcg cgcaggctgg
     1141 gcggaaacgc ctcgccgccg accgaggcct tgacgagcga gggcagcgcg acgccgagct
     1201 cgtcggcctt gtcgaggatg agcttcagga agctgggcgt gccgatgtag gccgcgggct
     1261 tcagtgccgc catcgcctgc acctgctgct cggtctgacc cgtgccgccg gggaacaccg
     1321 tgcagccgag cgcgaacgcg ccgctttcca tcatcgcgcc ggcgggcgtg aagtggtagc
     1381 tgaagctgtt gtgcaccagg tcaccggcgc gcagtcccgc ggcgaaaagc gcgcgcgcca
     1441 tgcgccagta gtcggggttc gcgccctcgg gttcgtagat cggccccggc gacgcgaaca
     1501 cgcgcagcgc gcggtgctcg gccgccgcgg cgccccaggc gagcgcagag aagccgccga
     1561 acacgtcggg agcatcggcg ccgcgctggc gctcgagcaa ctcgtgcttg cggatcaccg
     1621 gcaaccgagc cagcgcggcg cggctgttca ccgccgccgc gtcgacgccg gccaggctgg
     1681 ccgcgaaggc cggcgcgtgc gccctcgcat gcgcgagctg ggccggcagc gccgccatca
     1741 acgcggcctc gcgctgtgcc ggcgggcgtg cctcgagcgc gtcgtagtgt gtgctcattc
     1801 gtcatcaccg cgatcgacgc ggcgcttcgc gtcgtcgagc agcttgcgct gaccgaccgg
     1861 cgtgtcgacg gcatagcggt cgaatccgcc acgcgagcgc ttcagatcgc caaacagcgc
     1921 gcctgcgtcg tcgtgaaagt gcaggaacgc gctgcccttc agatagaaga tgccgggctt
     1981 catctcgcgc agacccttga tctcgcgcag ctggcgcagc aagggttgca ggcgttgcag
     2041 tgcggcgtcg tcggcatggg ccatcggcta tcctccttca cgcgagccag cgcttgcggc
     2101 gcttgtagct cttcacgtcc ttgaagctct tgcggtcgcc gccccccacg ccgaggtaga
     2161 actccttcac gtcctcgttc tcgcgcagcg aggccgccgc gccgtccatc acgatgcggc
     2221 cgttctcgag gatgtagccg tagtcggaat agcgcagcgc catgttggtg ttctgctcgg
     2281 cgagcaggaa ggtcacgccc tcgcgggtgt tcaggtcgcg cacgatctcg aacacctctt
     2341 cgacgatctg cggcgccagg cccatcgacg gctcgtcgag cagcaccatc ttcgggttcg
     2401 ccatcagcgc gcggccgatc gcgcacatct gctgctcgcc acccgaggtg taggcggcct
     2461 gcgaggcgcg ccgtgtcttg aggcgcggga aataggtgta gaccttgtcc agcgtggccg
     2521 cgaccgcggc cttgccatcg cggcgcgtgt aggcgccggt catcaggttc tcctggatcg
     2581 tcaggtgcgc gaagcagtgc cggccttcca tcacctggat cacgccgcgg ttcaccatct
     2641 cggacgtgct gagcgcctcg atgcgctccc cgcgcagttc gatcgacccc ttggtcacgg
     2701 cgccgcgttc gctctgcagc aggttgctga cggcgcgcag ggtcgtcgtc ttgccggcgc
     2761 cgttgccgcc catcagcgcg acgatgcgcc cctcgggcac ctgcagcgac acgcccttca
     2821 gcacgaggat cacatggttg tagatgacct cgatgccgtt gacgttgaga aggatgttgg
     2881 gcgacgcact catgttgttg cctttggctc atcgaaacgt cgcacgcggc gcttcgacga
     2941 gagcccctcg cgaagggcca tgccgcccgg cgagggccgg catggcagga gtcggccgat
     3001 cagctctgac aatcttcccc ggtgcggcgt accagcttct tctcggtcgc gtacttgccc
     3061 gccgaagcct tgaccatagg cttgatgatt gcgtcgtcgg cctgcagcca gtcggacgcc
     3121 cagacgaact tgctgccgtc ccaggtgtgc acgcgggccc aggccgaacc catgtggtcg
     3181 gtgcagctgg tggatatcgg gcgcaacacg ttcttgaagc ccagtgcgtc gagcttggct
     3241 tgggtgaggt tcaggttctc atagccccag cgtgcctgct cgccgctcat caccttgccc
     3301 ttgccgaagc gatcctgcgc cgcgcgcacg ccctcgacgg ccagcatcgc gccgaccacg
     3361 ccgcgcatat agatcacgct gccgacttcc tccttcggcc ccacgccctg acccgcgcca
     3421 tgcaccttgg ccaggatctc ctgcgccacc ggcgactgct gctcgttgcc gtgctgcatc
     3481 atcaccgcgc tgtagccctt ggcggcggca cccacgtctt tcaggtcggg ctcggcgccg
     3541 gaccaccagg tgccgaacat cttttcgcgc gggtaaccgg tggcgatggc ctccttgagc
     3601 gcggtgggcg tcatcacgcc ccaggtctgc atcacgacga agtcgggtcg ctgctggcgg
     3661 atctgcagcc agatcgcctt ttgctcgacg cccggcgcgg gcactggcag cagcagcagc
     3721 tcgaagccat gcgtggccgc gcgctcctgg atgatcggca gcagctcctt gccgaacggg
     3781 ctgtcgtggt agacgacggc gatcttctta cccttcaact tgtcccagcc gccttccttc
     3841 ttggcgatgt gctgcagcaa cgtgtcgccg gccacccagt agtggccgat cagcgggaag
     3901 ttccacttga aagtcaggcc gtccgccgag tcgctgcggc catagccgga cgtgatgaga
     3961 gggatcttgt cggcgggcac cttctcggtc agcgcaaagg tgatgccggt ggacagcggc
     4021 tggaacatcg tggcgccgcc atgcttgccc ttcaggcgct cgtagcactc gacacccttg
     4081 tcggtggcgt aggcggtttc gcattcctcg accaaggtct tcacgccatt gatgccgccg
     4141 cgtgcattga ccagcttcat gtagtcggcg aagccgttgg cgaacgcggt ggcgttgggc
     4201 gcgaccggtc cggtgcggcc ggtcaggctg gggaagaact ggaccttgtc ctgcgcaaat
     4261 gccgggccgg tcagcgccga ggcggcaatc gccgcagcga gggcgaggga cttcaagttg
     4321 aacatcatgg cgaatgcctc ctagggcgac aacgagggtg gcgcactctg cggtgcgtga
     4381 ggggttggaa cttcattcat caatgcggga acggccacag ccgcagcttt tccttggcga
     4441 tcgaccacag cttcgccagg ccatgcggct cgacgatcag gaagaacacg atcagcgcgc
     4501 cgaagatcat gttggtcagg tgcgaggcca gcgcggtgtc aatcggtatg ccgaaccagt
     4561 agggcaggtt gtcgagcacg atcggcagca ccacgatgaa ggcggcgccg aagaagccgc
     4621 ccatgatcga gcccaggccg ccgatgatga ccatgaacaa cagctggaat gagcggtcga
     4681 tgttgaacgc cgccggctcc cacgagccca ggtgcacgaa gccccacagc gcgccggaca
     4741 caccgacgat gaagctcgag accgcgaacg cggtgagttt ggcgtagacc gggcggatgc
     4801 cgatcacggc cgccgccaca tccatgtcgc ggatcgccat ccattcgcgg ccgatgtggc
     4861 tgcgcaccag gttcttcgcg agcagcgcga acaggcacgt gaacgccaga cagaacaggt
     4921 acttctgcgc cggtgtttcg atcgacagcc caaacacgtt gagcccggca acactgaccg
     4981 agcccgacga cgagttgttg gtgaaccact tgacgcgcag gaaggcccag tcgacgaaga
     5041 actgcgcggc cagcgtggcc actgccaggt acagaccctt gatgcgaagg ctcggaacgc
     5101 cgaacaccac gcccaccgcc gttgcgcaca ggcccgcgag caggatcgaa gccaccagcg
     5161 gcatgccttc gatgcgcacc tggaagttgt aggcagcgta ggcgcccacc gccatgaagg
     5221 cgccggtgcc cagcgaaatc tggccgcagt agccgaccag gatgttcaga cccagcgccg
     5281 cgagcgacag gatcaggaac ggaatcagga tggcgcggaa caggtattcg ggtgcgacga
     5341 gcggcaccac cagcaccgca aaggcgagca gcgcggcgat ggcgatgcga tcctgcgcga
     5401 tcgggaagat ctgctgatca gcgcggtagc tggtcttgaa ctggccgttc tcgcgataca
     5461 gcatggtcag acccgatcga tgatcttgcg gtgacggccc gctgcgccca actggcttcg
     5521 caagtcagcc tgcatcgaag aatcattgct ggcgttcata cgcgatcaat gattttttcg
     5581 ccgaacaagc cctggggcct gacgagcaag acgaacaaca ccagcacata gccgaaccag
     5641 atctcgatgc cgccgccgat ctgcggcccg aggtagacct ccgagacctt ttcgccgacg
     5701 ccgatgatca ggccaccgac gatggcgccc ggcacgctcg tcaggccgcc gaggatcacg
     5761 accggcagcg ccttcagggc caccagcgag agcgagaact gcacgccgag cttggaaccc
     5821 cagatgatcc cggccaccag cgcggtgaag ccggcgaccg accagacgat gacccagatg
     5881 cggttgagcg gaatgccgat cgactgcgcg gcctggtggt catcggccac ggcgcgcagc
     5941 gcgcggcccg tgccggtgta ctggaagaac aacgaaagca gcagcaccag cgcggcggcg
     6001 atcagcgcgg cgtacaggtc ttccttgctg accagcacgc cgccctggaa catcgattcg
     6061 aacaggaaga tcggatcctt gggcaggccg atgtcgatct tgtagatgtc gttgccgaag
     6121 atcgtctggc cgaagccgtc gagaaaatag gcgatgccca gcgtggccat cagcaatgtg
     6181 atgccctcct gattgaccag gcgcgacagg cacaggtgct cgatcagcca ggccatcgcc
     6241 accatcaccc ccatcgccac cgcgaacgcc agcaggttgg ccagcagctt gctgtcgaag
     6301 cccaggccct tgggaatcca ttcggaaaag cgcgccatcg ccagtgccga gaacagcacc
     6361 atcgcgccct gcgcgaagtt gaagacgccc gaggctttga agatcagcac gaagccgagc
     6421 gcgaccagcg aatacagcat gcccgtcatc aggccgccga ccagggtttc gaggaaggag
     6481 cccatgtcag tgcgaggtac cgaggtaggc cgaaatgaca tcgggattgg cgcgcaccgc
     6541 gtcgggcgca ccgtcgccga tcttcttgcc gtagtcgagc acgacgaccc ggtcgctgag
     6601 gtccatcacg acgcccatgt cgtgctcgat cagcacgatg gtcgtgccga actcgtcgtt
     6661 cacgtcgagg atgaagcggc tcatgtcctg cttctcctcg acgttcatgc cggccatcgg
     6721 ctcgtcgagc agcagcacct gcgggtccat cgccagcgcg cggccgaggt cgacgcgctt
     6781 ttgcaggccg tacggcatct tgcccaccgg cgtcttgcgg tagggctgga tctcgaggaa
     6841 gtcgatgatg cgttcgacga tcgcgcgctg cgcgatctcc tcgcgctcgg ccgtgccgaa
     6901 cccgaatggg ttgcgaaagg cctgctgcag cagactcgcc ttcatcttca ggttgcg
//
LOCUS       DATMJB010000244         2423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_784080, whole genome shotgun sequence.
ACCESSION   DATMJB010000244 DATMJB010000000
VERSION     DATMJB010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784080"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VNU48_05405"
     CDS             complement(<1..769)
                     /locus_tag="VNU48_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HWI10747.1"
                     /translation="MAKIIGIDLGTTNSCVSIMEGNTTKVIENSEGARTTPSIVAYQD
                     DGEILVGASAKRQAVTNPKNTLYAVKRLIGRKFTEKEVQKDIDLMPYKITAADNGDAW
                     VEVRGNKIAPQQVSAEILRKMKKTAEDYLGEVVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGLDKKEGDRKIAVYDLGGGTFDISIIEIANVEGEHQFEV
                     LSTNGDTFLGGEDFDLRIINFLAEEFQKESGVDVRKDP"
     gene            complement(861..1418)
                     /gene="grpE"
                     /locus_tag="VNU48_05410"
     CDS             complement(861..1418)
                     /gene="grpE"
                     /locus_tag="VNU48_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWI10748.1"
                     /translation="MTNIDPNAPPPEPAVSAAPAQEAAAPAQEAAAPVNAETRLQELE
                     ARHAEVSDAYLRAKAEAENTRRRADDEIAKARKYAVEGFADSLLPVKDSLEAAIAIPD
                     ASPGQMLEGIHATLRQLVAALERNKVIEINPASGVKFDPHQHQAISVVPAPQEPNTVV
                     AVLQKGYLIADRVLRPALVTVAAAA"
     gene            complement(1520..1939)
                     /locus_tag="VNU48_05415"
     CDS             complement(1520..1939)
                     /locus_tag="VNU48_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HWI10749.1"
                     /translation="MKESSRTDKVRLDKWLWAARFFKTRSLSADEIGRGRVQVNGQAA
                     KASREVRVGDTIALKQGQVARTVVVTAASEQRGPATEAQGMFTETPESIERRTQEALA
                     RRLKAEPSLAIEQGRPTKRDRRQLADWNRWSASAEPE"
     gene            complement(1982..>2423)
                     /locus_tag="VNU48_05420"
     CDS             complement(1982..>2423)
                     /locus_tag="VNU48_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HWI10750.1"
                     /translation="LGDPYHCECLATARLLGERLDLAKDGLLVTFQSRLGRAEWLQPY
                     TEPTLIALAKQGVRSVDVMCPGFTSDCLETLEEIDQQARNAFLRAGGTTFNYLPCLND
                     QHEWMAALSAVAIRHLQGWPTTSRPDATALEAQRQRARAAGAAQ"
BASE COUNT          299 a          790 c          846 g          488 t
ORIGIN      
        1 gcgggtcctt gcgcacgtcg acgccggact ccttctggaa ctcctcggcc aggaaattga
       61 tgatgcggag gtcgaagtcc tcaccgccga ggaacgtgtc gccgttggtc gatagcactt
      121 cgaactgatg ctcgccttcg acgttggcga tctcgatgat cgagatgtcg aacgtgccgc
      181 cgcccagatc gtagaccgcg atcttgcgat cgccttcctt cttgtcgagc ccgaacgcca
      241 gcgctgcggc ggtcggctcg ttgatgatcc gcttgacgtc gagtccggcg atgcggccgg
      301 cgtccttcgt cgcctggcgc tgtgcgtcgt tgaagtacgc gggcaccgtg atgacggcct
      361 cggtgacgac ttcaccgaga tagtcctcgg cggtcttctt catcttgcgc aggatttccg
      421 cgctgacctg ctgcggcgcg atcttgttgc cgcgcacctc gacccacgcg tcgccgttgt
      481 cggccgcggt gatcttgtag ggcatcaggt cgatgtcctt ctgcacttcc ttctcggtga
      541 acttgcggcc gatcaggcgc ttcaccgcgt acagcgtgtt cttcgggttg gtgacggcct
      601 ggcgcttggc cgaggcgccg acgaggatct cgccgtcgtc ctggtacgcg acgatcgacg
      661 gcgtggtgcg cgcaccctcg ctgttctcga tcaccttggt ggtgttgcct tccatgatcg
      721 acacgcacga gttcgtggtg ccgaggtcga tgccgatgat tttggccatg tggtgtgctc
      781 ctgagactga atgagaggtt gttctcaacg tgtggataag gtgtgggctt tcaagcctga
      841 acctgtggag ttctagtggc tcaggccgcc gctgccacgg tgaccagcgc cggccgcagc
      901 acgcggtcgg cgatcagata gcccttttgc agcaccgcga ccaccgtgtt gggttcctgc
      961 ggcgccggca ccacgctgat ggcctggtgc tggtgcggat cgaacttcac gcccgaggcc
     1021 gggttgatct cgatcacctt gttgcgctcg agcgcggcga ccagctggcg cagggtcgcg
     1081 tggatgccct cgagcatctg cccggggctt gcatccggga tcgcgatcgc cgcctccagg
     1141 ctgtccttga ccggcagcag gctgtcggca aagccctcga cggcgtactt gcgcgccttg
     1201 gcaatttcgt cgtcggcgcg ccggcgcgtg ttctcggcct cggccttggc acgcaggtag
     1261 gcgtcagaga cttcggcgtg gcgcgcctcg agttcctgca gccgggtctc ggcattgacg
     1321 ggggcggcgg cctcctgtgc gggcgcggcg gcctcctgcg cgggggcggc gctgacggcc
     1381 ggttcggggg gcggtgcgtt cgggtcgatg ttcgtcatgg cgtcaaagga tttccgtagc
     1441 gccccaactg aggcgattgc cctcagattc aagaggggat tgcggagatt ttccggggtt
     1501 cagtgctcgt cggccccggt cactcgggtt cggccgaggc gctccagcgg ttccagtcgg
     1561 cgagctggcg ccggtcgcgc ttggtcgggc ggccttgctc gatcgccagc gacggttccg
     1621 ctttcagacg ccgtgccagc gcttcctgcg tgcggcgctc gatgctctcc ggcgtttccg
     1681 tgaacatgcc ctgcgcctcg gtcgccgggc cgcgctgctc gctcgcggcg gtcacgacga
     1741 cggtgcgcgc gacctgaccc tgcttcagcg caatcgtgtc gccgacgcgc acctcgcgcg
     1801 acgccttcgc cgcctggccg ttgacctgca cccggccgcg gccgatctcg tcggcgctga
     1861 ggctgcgggt cttgaagaag cgggcggccc acagccattt gtcgaggcgc accttgtcgg
     1921 ttcgggaact ttctttcatc gagggtgatt gtcgttcgcg tcggacgccc ggccaacccg
     1981 gctattgcgc ggccccggcg gcgcgcgcgc gctggcgctg cgcctcgagg gccgtggcat
     2041 cgggccggct ggtcgtcggc cagccctgca gatgccggat cgcgaccgcg gacagcgccg
     2101 ccatccactc gtgctggtcg ttcaggcagg gcaggtagtt gaaggtggtg ccgccggcgc
     2161 gcaggaaggc gttgcgggcc tgttggtcga tctcttccag cgtctccagg cagtccgagg
     2221 tgaaccccgg gcacatcacg tcgacgctgc gcacgccttg cttggcgagc gcgatcaggg
     2281 tcggctcggt gtagggctgc aaccactcgg cgcggccgag ccggctctgg aacgtgacca
     2341 gcaggccgtc cttggcgagg tcgagccgct cgccgagcag ccgcgcggtc gccaggcatt
     2401 cgcagtggta cgggtcgccc agc
//
LOCUS       DATMJB010000245         3830 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_840296, whole genome shotgun sequence.
ACCESSION   DATMJB010000245 DATMJB010000000
VERSION     DATMJB010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840296"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="VNU48_05425"
     CDS             complement(<1..826)
                     /locus_tag="VNU48_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HWI10751.1"
                     /translation="MNPDAHTLWRAPRWALAVLLACLGMLGPFSIDTYLPAFTGIASA
                     IGATPVEMQQTLSAYLFGFAIMNLFHGALSDSFGRRPVVLTGLAVFTLASVGCAMSQH
                     IGALVFFRAVQGMSAGAGIVISRAVIRDMFPPADAQRVMSQVTIYFGVAPAIAPMVGG
                     FLFVHVDWHAIFWFLTAVGVVLWIANYKLLPETLHRSHRQSFRARHLLRGYWEMGSSA
                     RFMMLALASGIPFNGMFLYVLSAPVFLGEHLHLAPTQFYWFFMLTIGGIMGGAWFSG"
     gene            complement(974..1334)
                     /gene="rnpB"
                     /locus_tag="VNU48_05430"
     ncRNA           complement(974..1334)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VNU48_05430"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            1590..1988
                     /locus_tag="VNU48_05435"
     CDS             1590..1988
                     /locus_tag="VNU48_05435"
                     /inference="COORDINATES: protein motif:HMM:NF025045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HWI10752.1"
                     /translation="MTMKNALTVVAAVAAAMFAQATFAQASAPASRAEVKAETKAAVK
                     AGAVTPAGEATPVEKSTTKSTKTRAERKAETMAAKKDKKLTPAGEGSPAAEKASGPSA
                     ARADVKADTKAAVKAGTTTPAGEGPGAPKK"
     gene            complement(2077..2826)
                     /locus_tag="VNU48_05440"
     CDS             complement(2077..2826)
                     /locus_tag="VNU48_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWI10753.1"
                     /translation="MNNTRVALLIDADNLSGDVIEQAVEHLLKLYGAVHFRRAYSSPQ
                     KVIEHTELFRRHSIRPMVNVPTGKNSTDIALAVDAIDLAICENPDVVVIASSDSDYAP
                     LAQRLRERGCRVLGIGQAGKTGSESPLAYDEFIDLTHRKSRGNEVGAADARRTRARPP
                     AARARLPAPVAAAPAALPPEVASILQAVPALRSGAALQLGEVGEALRRHEILTSKRAS
                     ASGFLKRHAAHFELLPSDRPRTVRYRGPKSG"
     gene            complement(2827..>3830)
                     /gene="pyrC"
                     /locus_tag="VNU48_05445"
     CDS             complement(2827..>3830)
                     /gene="pyrC"
                     /locus_tag="VNU48_05445"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWI10754.1"
                     /translation="GAALASVVPDSARQFGRAIIMPNLKPPVTTAVQAVAYRARILAA
                     VPEGMAFEPLMTLYLTDTLPPDEIARAQAAGVVALKLYPAGATTNSDAGVTELRKTYP
                     TLEAMQRAGLPLLVHGEVTDAEIDLFDREKAFIDTRLIPLRRDFPELKIVFEHITTRE
                     AAQYVSEADRFVGATVTAHHLLYNRNAIFLGADGSSGLRPHYYCLPVLKREEHRRALV
                     AAVTSGSTKFFLGTDSAPHAAALKEHALGCAGCYTARSALELYVEAFDAADALPRFEA
                     FASFNGPAFYGLPRNAGRVTLRRERWTLPETLPFGEATLKPLRGGESLAWRQLRRKP"
BASE COUNT          615 a         1300 c         1348 g          567 t
ORIGIN      
        1 ggccgctgaa ccacgcgccg cccatgatgc cgccgatcgt cagcatgaag aaccagtaga
       61 actgcgtcgg ggccaggtgc aggtgctcgc cgaggaagac cggcgccgac agcacataca
      121 ggaacatgcc gttgaacgga atgccgctgg ccagcgccag catcatgaag cgcgcgctgg
      181 agcccatctc ccagtagccg cgcagcaggt ggcgcgcgcg aaaggactgc cgatggctgc
      241 ggtgcagcgt ctcgggcagc agcttgtagt tggcgatcca caggaccacg ccgaccgcgg
      301 tcaggaacca gaagatcgcg tgccagtcga catgcacgaa caggaagccg ccgaccatcg
      361 gcgcgatcgc cggggccacg ccgaagtaga tcgtcacctg cgacatcaca cgctgcgcgt
      421 cggcgggcgg gaacatgtcg cggatcactg cgcgcgagat cacgatgccg gcgccggccg
      481 acatgccctg caccgcgcgg aagaacacca gcgcgccgat gtgctgcgac atcgcgcagc
      541 ccaccgaggc gagcgtgaac accgccagcc cggtcagcac caccgggcgg cggccgaagc
      601 tgtcggacag cgcgccgtgg aacaggttca tgatcgcgaa cccgaacagg taggccgaca
      661 gcgtctgctg catctcgacc ggcgtggcgc cgatcgcgct ggcgatgccg gtgaaggccg
      721 gcaggtaggt gtcgatcgag aacggcccca gcatgcccag gcaggcgagc aggacggcca
      781 gcgcccagcg cggcgcgcgc cagagagtgt gtgcgtcggg attcatcgaa cggccgagtc
      841 taggtgagcg ccgccgcctc gacgctgcgg ccctgctcgc ctatgctgca ttggccgtca
      901 agcggcgaag gcaatggcgc gcggacggtg cgcgagcgtc tgaacccgcg gcctctgtga
      961 actcgagaga aaagtgtgaa gcgggccgat aggccggatt ctgtgcgacc gtcgcctctt
     1021 gcgaagcgcc ggtcgtgacc gccattcctc tgggccgtgc atcgctgccc ggctcggtgc
     1081 cacctacccg ccagctcgtg cgggaccaca cttcaagacg gacgcggtcg ctgccgcgcc
     1141 gctcacgctg gcctatttgg tgttgctgcg cgtagagatt gcccgtttca cccgaactga
     1201 atcggctcgt ctctgttgct ctgatcctca cctcacggtg gacagccgtt agctgctacg
     1261 ccgccctgcg cagtccggac cttcctccag tgcgcccttt cgggccttgc accagcggcg
     1321 gtctgacccg cttcacagtc gcgattatgt cggccgatcg cgtcgtgtgc ggcatcggcg
     1381 caacaaatcg gcggtccgcg gcgggcggtc gcgcttgaaa ttcgagtttc gtcaaccgat
     1441 gcggcgatcc cgcgttctaa tacgtgccgc gctccaccgg tctgcaactg cggcccgaca
     1501 gcaagccgac ctgatgaggg catgtcgctc gagtgcgctg cgctttttcg tgaactgccc
     1561 ttaccctcgt tctcaaacag gagattgata tgacgatgaa aaacgctctg accgttgtgg
     1621 ccgctgtcgc cgctgccatg ttcgcccaag ccacgttcgc ccaggcatcg gccccggcgt
     1681 cgcgcgccga agtcaaggcc gaaacgaagg ccgccgtgaa ggcaggcgcc gtcaccccgg
     1741 caggcgaagc cacaccggtg gaaaagtcga ccaccaagtc gaccaagacc cgtgcggagc
     1801 gcaaggccga gacgatggcc gccaagaagg acaagaagct gaccccggcc ggcgaaggct
     1861 cgccggctgc cgagaaggcg agcggcccga gcgcagcgcg cgccgatgtg aaggccgaca
     1921 ccaaggccgc cgtgaaggcc ggcacgacga ctcccgctgg cgaaggcccg ggcgccccga
     1981 agaagtaagg agcctcccga gcgccgagtc acttcggcgc ccgagggttc ggaacagcgg
     2041 cctgcgggcc gctgtttgtt tttgggccgc gcggagctat cccgacttcg ggccgcggta
     2101 gcgcacggtg cgcggccggt cgctcggcag cagctcgaaa tgcgccgcgt ggcgcttcag
     2161 gaagccgctc gccgaggcgc gcttgctggt caggatctcg tggcggcgca gcgcctcgcc
     2221 gacctcgccg agctgcagcg cggcaccgct gcgcagcgcc ggcacggcct gcaggatgct
     2281 cgccacctcg ggcggcaacg ccgccggggc cgctgcgacc ggcgcaggca gcctggcgcg
     2341 cgctgccggc ggacgcgccc gcgtccgccg ggcatcggcc gcacccacct cattgccgcg
     2401 gctcttgcgg tgcgtcagat cgatgaactc gtcgtaggcc agcggcgact cgctgccggt
     2461 cttgccggcc tggccgatgc cgagcacgcg acagcccctc tcgcgcaggc gctgcgcgag
     2521 cggcgcgtag tcggagtcgc tcgacgcgat cacgaccacg tcggggtttt cgcagatcgc
     2581 caggtcgatt gcgtcgacgg ccagcgcgat gtcggtcgag ttcttgccgg tcggcacgtt
     2641 gaccatcggc cggatcgagt ggcggcggaa cagctcggtg tgctcgatga ccttctgcgg
     2701 gctcgagtac gcgcgccgga aatggactgc gccgtagagt ttgagcaggt gctcgacggc
     2761 ctgctcgatg acatcgcccg agaggttgtc ggcatcgatc agcagcgcca cgcgggtgtt
     2821 gttcatttag ggctttcgtc gaagctggcg ccaggccagg ctttcgccgc cgcgcagggg
     2881 tttcagggtg gcctcaccga agggcaaggt ctcgggcagc gtccagcgct cgcgccgcaa
     2941 ggtcacgcgg cccgcgttgc gcggcaggcc gtagaacgcc gggccgttga agctcgcgaa
     3001 ggcctcgaaa cgcggcagcg cgtcggccgc gtcgaaagcc tcgacgtaga gctcgagcgc
     3061 cgagcgcgcg gtgtagcagc cggcgcagcc gagcgcgtgt tccttcagcg ccgcggcgtg
     3121 cggcgcgctg tcggtgccga gaaagaactt ggtgctgccc gacgtgaccg cggcgaccag
     3181 cgcgcgacgg tgctcctcgc gcttgagcac cggcaggcag tagtagtgcg ggcgcagtcc
     3241 tgacgaccca tccgcgccga ggaagatcgc gttgcggttg tagagcaggt ggtgcgcggt
     3301 cacggtcgcg ccgacgaagc ggtcggcctc gctcacgtac tgcgccgcct cgcgcgtcgt
     3361 gatgtgttcg aagacgatct tcagttcggg aaagtcgcgc cgcagcggga tcagccgggt
     3421 gtcgatgaac gccttctcgc ggtcgaacag gtcgatctcg gcatcggtca cctcgccgtg
     3481 caccagcagc ggcaggccgg cgcgctgcat cgcctcgagc gtcgggtagg tcttgcgcag
     3541 ctcggtgacg ccggcgtcgc tgttggtcgt cgcgccggcc gggtagagct tgagcgcgac
     3601 cacgccggcc gcctgcgcgc gcgcgatctc gtcgggcggc agcgtgtcgg tcaggtacag
     3661 cgtcatcagc ggctcgaagg ccatgccctc gggaacggcc gcgaggatgc gcgcgcgata
     3721 ggcgaccgcc tgcaccgccg tcgtcaccgg cggcttcagg ttcggcatga tgatcgcgcg
     3781 gccgaactgg cgcgcgctgt cgggcacgac cgacgcgagc gcggccccgt
//
LOCUS       DATMJB010000246         2134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_560848, whole genome shotgun sequence.
ACCESSION   DATMJB010000246 DATMJB010000000
VERSION     DATMJB010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560848"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VNU48_05450"
     CDS             complement(<1..572)
                     /locus_tag="VNU48_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWI10755.1"
                     /translation="MQLNPDDITRAAALLAALRDEVGKAVVGQHAVVDQTLVALVASG
                     HVLIEGVPGLGKTLLARALAKALAIDGARVQFTPDLMPSDITGHTVLEPGAGGAVDLG
                     ALRVRRGPVFTNLLLADEINRAPAKTQSALLEVMQEYQVTIDGQTLPLAKPFMVLATQ
                     NPLETEGTYPLPEAQLDRFLLKIVLPIPER"
     gene            complement(590..1864)
                     /locus_tag="VNU48_05455"
     CDS             complement(590..1864)
                     /locus_tag="VNU48_05455"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HWI10756.1"
                     /translation="MTLTRQHGVTALVAAVLVAGAVWFARHTEWVDEEVRDPPSAELR
                     SDPNLRLKRVLARAGAEIATPDNLERLPPRGATLVLSSWNWDLFPERSAALRRWVEAG
                     GDLVVPNFGFRRDGLAWVPVKWRPMRRPAGVAPAASAAHETDEDDDQDDDDTPRRAPP
                     AAKFDPSTRLRPTPCRGVNEPAGVTPAFGLPRAYATCLWLEQTLHTAAPLLWALDGPN
                     GHVVVRVPVGRGSVTLSSAPLPWDNETVLERDNALIAVAATRAQPGRAIWLVTDEVRE
                     PLLAFLWSHGAPAILLGAAALAFALWRGALRFGPRIAAAPLARRSMAEQIRGTAAFIA
                     RRGSPALHAAELRALNDAATPRIRGFAAMVLGQRAQAIADRTGLDAHALAHALNPALH
                     ATPTRHPAAALALLETARRRLVNRPQAPDASH"
     gene            complement(1861..>2134)
                     /locus_tag="VNU48_05460"
     CDS             complement(1861..>2134)
                     /locus_tag="VNU48_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HWI10757.1"
                     /translation="RGALSRLVHRHGVAIRAASTEGECVRLAQRALPAEAGAFFERLV
                     QVWQTEVYAGRAAEGAALAALCDAFDRHFGTPAAAGVPASSGPAHA"
BASE COUNT          263 a          770 c          828 g          273 t
ORIGIN      
        1 tgtcgctcgg ggatcggcag cacgatcttg agcaggaagc ggtcgagctg tgcctcgggc
       61 agcgggtagg tgccttcggt ctcgagcggg ttctgggtgg ccagcaccat gaagggcttt
      121 gccagcggca gcgtctggcc gtcgatcgtg acctggtact cctgcatcac ctcgagcagc
      181 gcgctctgcg tcttcgccgg cgcgcggttg atctcgtcgg cgagcagcag gttcgtgaac
      241 accgggccgc gccgcacgcg cagcgcgccg aggtcgacgg cgccgccggc cccgggctcg
      301 agcacggtgt ggccggtgat gtccgagggc atcaggtccg gcgtgaactg cacgcgcgcg
      361 ccgtcgatgg ccagcgcctt ggccagcgcg cgcgccagca gcgtcttgcc caggcctggc
      421 acgccctcga tcaacacatg gccggaggcg accagcgcga ccagcgtctg gtcgacgacg
      481 gcatgctggc cgaccacggc cttgcccact tcgtcgcgca gcgctgcgag cagcgcggcg
      541 gcgcgcgtga tgtcgtcggg gttgagttgc atgggagccg gtccttttct cagtgggatg
      601 cgtcgggtgc ctgcgggcgg ttcaccagcc ggcgccgtgc ggtttcgagc agggcgagcg
      661 ccgccgcagg gtggcgcgtc ggcgtggcgt gcagcgccgg gttcagcgcg tgggcgaggg
      721 cgtgcgcatc caggccggtg cgatcagcga tggcttgcgc gcgctggccg agcaccatcg
      781 ccgcgaagcc gcggatgcgt ggcgtggcgg cgtcgttcag cgcgcgcagc tcggccgcat
      841 gcagagccgg gctgccgcgc cgggcgatga aggcggcggt gccgcggatc tgctcggcca
      901 tcgagcggcg cgccagcggc gcggcggcga tgcgcgggcc gaagcgcagc gcgccgcgcc
      961 acagcgcgaa cgcgagcgcg gcggcgccga gcaggatcgc cggcgcgccg tggctccaca
     1021 gaaacgcgag cagcggctcg cgcacctcgt cggtgacgag ccagatggcg cgccccggct
     1081 gcgcgcgcgt cgccgccacc gcgatcaggg cgttgtcgcg ttccagcacg gtttcgttgt
     1141 cccagggcag cggcgcgctc gacaaggtga cgctgccgcg gcccaccggc acgcgcacga
     1201 cgacatgacc gttcgggccg tcgagcgccc acagcagcgg cgcggcggtg tgcagcgtct
     1261 gctccagcca caggcaggtc gcgtaggcgc gcgggaggcc gaaggccggc gtcacgccgg
     1321 cgggttcgtt gacaccgcga cagggcgtgg gtcgcagccg cgtcgacggg tcgaacttcg
     1381 cggccggcgg cgcgcgccgc ggcgtgtcgt cgtcgtcttg gtcgtcgtcc tcgtcggtct
     1441 cgtgcgccgc ggacgcggcg ggcgccacgc cggccgggcg ccgcatcggc cgccatttga
     1501 ccggcaccca ggccagcccg tcgcggcgga agccgaagtt cggcacgacc aggtcgccgc
     1561 cggcctcgac ccagcggcgc agcgccgccg aacgttcggg gaacaggtcc cagttccacg
     1621 agctgagcac cagcgtcgca ccgcgcggcg gcagccgttc gaggttgtcg ggcgtggcga
     1681 tctcggcgcc ggcacgcgcg agcacacgtt tcaggcgcag gttcgggtcg ctgcgcagct
     1741 cggcgctcgg cgggtcgcgt acctcctcgt cgacccattc ggtgtggcgc gcgaaccaga
     1801 ccgcgcccgc gaccagcacc gcggcgacca gcgccgtcac gccgtgctgg cgggtcagcg
     1861 tcatgcgtgt gccgggccgg aagacgccgg cacgcccgcc gctgccggag tgccgaagtg
     1921 ccggtcgaac gcgtcgcaca gcgcggcgag cgccgcgccc tcggcggcgc ggccggcata
     1981 gacctcggtc tgccagacct gcaccagccg ctcgaagaac gcgccggcct cggccggcaa
     2041 cgcgcgctgc gcgagccgca cgcactcgcc ctcggtgctg gcggcgcgga tcgccacgcc
     2101 gtgccggtgc accagccgcg acagcgcgcc gcgg
//
LOCUS       DATMJB010000247         2156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_133091, whole genome shotgun sequence.
ACCESSION   DATMJB010000247 DATMJB010000000
VERSION     DATMJB010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133091"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /gene="aroQ"
                     /locus_tag="VNU48_05465"
     CDS             <1..435
                     /gene="aroQ"
                     /locus_tag="VNU48_05465"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HWI10758.1"
                     /translation="GPNLNLLGTREPQVYGADTLADVEAMAATLATQRGVALECLQSN
                     HEGALIDRIHAARLDGTDCIVINPGGLTHTSVALRDALAGVAIPFIEVHVSNVHAREA
                     FRHHSFLSPLAAGVIVGFGVAGYAMALNAAIDRLVAARAPGG"
     gene            540..929
                     /locus_tag="VNU48_05470"
     CDS             540..929
                     /locus_tag="VNU48_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10759.1"
                     /translation="MHPTRLLIVPVSAALLSACVVAPYPGRVVYSQPVPAYAQPGPVY
                     SQPVPGDGDQAVVDIAPPAPYVEVVPAIPFAGAIWIGGYWGWRGGRHAWVPGRWEHPR
                     AGYAWHPHAWVHEGGRWHLHGGGWVRH"
     gene            1353..>2156
                     /locus_tag="VNU48_05475"
     CDS             1353..>2156
                     /locus_tag="VNU48_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HWI10760.1"
                     /translation="MIADRLIHHPGNMPKVHSHYENLKVARDASPEAIRAAYRALTRK
                     HHPDRHPDSAEAQRIMSVVNVAYAVLSDPAKRREHDQWIRQAEGPVARPLRDRHTLHA
                     PTAAYASAAADAYARRARQALLVERAARLKAHVLGHRAAYALAALAAFGLLIGSVRAL
                     LEPDLGARITAPVAATTRYAGYVRPPSAPNGHAWPTRSGYVDGYPQLNRGGLSDVTID
                     NSRNDADMFAKLVSLDGPIALPVRTVLVTAHGRFKITGLSIGTYDLRYRN"
BASE COUNT          301 a          796 c          756 g          303 t
ORIGIN      
        1 ggacccaacc tgaacctgct cggcacgcgc gagccgcagg tctatggtgc cgacaccctg
       61 gccgacgtcg aagcgatggc cgcgacgctc gccacgcagc gcggtgtcgc gctcgaatgt
      121 ctgcagagca accatgaagg cgcgctgatc gatcgcatcc acgcggcgcg gctcgacggc
      181 accgactgca tcgtcatcaa ccccggcggc ctgacccaca ccagcgtggc gctgcgcgat
      241 gcgctggccg gcgtggcgat tccgttcatc gaggtgcatg tgtcgaacgt gcatgcgcgc
      301 gaggcgttcc gacaccactc gttcctgtcg ccgctcgccg ccggcgtgat cgtcggtttc
      361 ggcgtggccg gttatgcgat ggcgctgaac gccgcgatcg accggctcgt cgccgcccgg
      421 gcgccgggag gctgaaaatt tctgtaaagg atgccgtgtc gtgtcggcgg cccggcggcc
      481 tgggcagtta gacaggcatg ggccgcccgt gccgcggcgg ccgctcgcgg agaatcgcca
      541 tgcatccaac cagactccta atcgttcccg tttcggccgc gctgctgtcg gcctgtgtcg
      601 tcgcaccgta tccggggcgg gtggtgtatt cgcagcccgt tccggcctat gcgcagccgg
      661 ggcccgtgta ctcgcagccg gtgccgggcg acggcgacca agccgtcgtc gacatcgcgc
      721 cgccggcccc gtacgtcgaa gtcgtgccgg cgatcccgtt cgccggcgcg atctggatcg
      781 gcggctactg gggctggcgc ggcggccgcc acgcctgggt gccggggcgc tgggagcacc
      841 cgcgcgcggg ttacgcgtgg cacccgcacg cctgggtcca tgaaggcggg cgctggcacc
      901 tgcacggcgg cggctgggtc cggcactagg agcctgggcg gcagagagcc gggccccctt
      961 gcaggcccaa cccttcgggc cggggcgata tgccgtccag gcccctagcc ctgcagggcc
     1021 tcccagaccg ccgcggtcgt gttgaccgca tgctgcgcga cctgcgcagc gctgcccgcc
     1081 agcaccgtca acgggtaggt gcccgagacg atgccgccac acgcgacctc gaccgcccgg
     1141 acctcgggcg gcaggccctc gaccgtcggc gacgacgcgg tactcgtaga cgtcgtcggc
     1201 gcggtggatc tcgattcgaa cgatcatgct cactcccccg gggtgtgcgg cgcgccgctg
     1261 cgtctgtgcc ccatgtgatt ctgatcgaag cgccgcgcgg cgccgctggc ggcaacgcga
     1321 aagcctcgtg cggccgtgcg ccgatgtgag ccatgatcgc ggatcgcctc atccaccacc
     1381 ccggcaacat gccgaaagtc cacagccact acgagaacct gaaggtcgcg cgcgacgcgt
     1441 cgcccgaggc gatccgcgcg gcctaccggg ccctgacgcg caagcaccac cccgaccgcc
     1501 accccgacag cgccgaggcg cagcgcatca tgtcggtggt caacgtcgcg tacgcggtgc
     1561 tgtccgaccc cgcgaagcgg cgcgagcacg accagtggat ccgccaggcc gaggggccgg
     1621 tcgcgcggcc gctgcgcgac aggcacacgc tgcacgcgcc gaccgccgcg tacgcgtcgg
     1681 ccgccgccga cgcgtacgcg cggcgtgccc ggcaggcgct gcttgtcgag cgcgcggccc
     1741 ggctcaaggc gcacgtgctc gggcatcgcg cggcctatgc gcttgccgcg cttgccgcgt
     1801 tcggtctgct gatcggctcg gtgcgggcgc tgctcgagcc ggacctgggc gcgcgaatca
     1861 ccgcaccggt cgccgccacc acgaggtacg ccggctacgt gcggccaccg agcgcgccga
     1921 acgggcatgc atggccgaca cgctccggct atgtcgacgg ctacccgcag ctgaaccgcg
     1981 gcgggctgtc ggacgtgacg atcgacaact cgcgcaacga cgcggacatg ttcgcgaagc
     2041 tggtgtcgct ggacgggccg atcgcgctgc cggtgcgcac ggtactcgtc acggcgcacg
     2101 gccgcttcaa gatcaccggc ctgagcatcg gcacctacga cctgcgctac cgcaac
//
LOCUS       DATMJB010000248         3735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_839746, whole genome shotgun sequence.
ACCESSION   DATMJB010000248 DATMJB010000000
VERSION     DATMJB010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839746"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VNU48_05480"
     CDS             <1..244
                     /locus_tag="VNU48_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWI10761.1"
                     /translation="ELAPDGNGEALLKAARWPDASEPVLIVGHQPTLGFVASYLLADQ
                     PRPWTIKKGAVWWIRGRNREDREQVILQAVQSPDCL"
     gene            complement(248..1780)
                     /locus_tag="VNU48_05485"
     CDS             complement(248..1780)
                     /locus_tag="VNU48_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HWI10762.1"
                     /translation="MPESPSRPGPAEGPRWPGQLAAIDMGSNSFRLEIGQLIDGRYRR
                     IDYQKETVRLGAGLDANGLLTDAAGARGLACLARFAQRLKGFTPAQVRAVATQTLREA
                     RNRDAFLIRAQGALGFPIEVISGREEARLIYAGVARLQPSAVPRLVVDIGGRSTEMIL
                     GHGSRPLRAESFQVGSASLSMRYFGDGRFTEAAFRQAQIAAGAELEEALEPFAAVNWQ
                     EALGSSGTVGAVSQLLAASGITDGRITPEGLRWLMQQCLRAGKVDKLVLPGLKEERRA
                     VIAGGLSILYTLAANFGIEALLPARGALRQGVIFDLDERSKAAEQRQDGHDIRDASVR
                     ELQRRFMVDTQQAARVSGVAQALYASVQPGAGAEMRRELLWACALHEIGMMISHHDHH
                     RHSAYVLDHVDAPGFSQSQLRRLGALVLGQRGGLRKIETSLTRDDFAWQVLCLRLGAI
                     KCHARGDVGTDAIRLQRDAATAALSFDAGWTDSHPRTRHLLEEEAAMWARSGPLKLEL
                     RR"
     gene            complement(1911..2345)
                     /locus_tag="VNU48_05490"
     CDS             complement(1911..2345)
                     /locus_tag="VNU48_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWI10763.1"
                     /translation="MFKRILVPTDGSDITVKAIAAAIELARSLSAQLYTISVKEPFPY
                     SAISEMQPTPPQEFFDAQERIAAKRVQAVLDLAQKAGVPCQSHTVEALHPWEAIIEHA
                     QRNECDLLVMASHGRRGVSALLLGSETQKVLTHSKVPVLVVR"
     gene            complement(2474..3325)
                     /locus_tag="VNU48_05495"
     CDS             complement(2474..3325)
                     /locus_tag="VNU48_05495"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HWI10764.1"
                     /translation="MPYTTLITIDELRAALAGANAPLLIDTSFDLADVDAGEASWRAG
                     HLPGAIYLHMDRDLSGAKTGRNGRHPLPTRAAFAATLGRCGVTPSTQVVAFDRQGGVY
                     AARLWWMLRWMGHAAAAVLDGGVAAWTAGGGALSTDDTPMRAALAPYPEHASLVGTLD
                     ADALLAALGRVRLLDARAGERFRGEVEPLDATAGHIPGALNRFFKDNLAADGRFKPAA
                     QLRADFTGLLGTQTAADTVHQCGSGVTACHNLLAMEHAGLTGSLLYPGSWSEWASDPA
                     RPVARSG"
     gene            complement(3327..>3735)
                     /locus_tag="VNU48_05500"
     CDS             complement(3327..>3735)
                     /locus_tag="VNU48_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HWI10765.1"
                     /translation="LQVTALARAGEPEMRIVFYFSLGGAIFGLITTLYSGIHQHSLKG
                     AMLLLAVGLLATVAQLLMTRAYSTGRTLVIASLQYLGIAFSFGYGVLLFADRMTWMAF
                     AGMLLIVGAGLAATRLRSRTAAAADAVNSTLES"
BASE COUNT          518 a         1327 c         1368 g          522 t
ORIGIN      
        1 cgagctcgca cccgacggca acggcgaggc cttgctgaag gcggcgcgct ggcccgacgc
       61 gagcgagccg gtgctgatcg tcggccacca gccgacgctg ggcttcgtcg cctcgtacct
      121 gctcgcggac cagccgcgcc cgtggacgat caagaagggc gcggtctggt ggatccgcgg
      181 gcgcaaccgc gaagaccgcg agcaggtgat cctgcaggcc gtgcagtcgc ccgattgcct
      241 ctgaacctca gcgccgcagc tccagcttca gcgggccgct gcgcgcccac atcgccgcct
      301 cctcctccag caggtggcgg gtgcgcgggt ggctgtcggt ccagccggcg tcgaagctga
      361 gcgcggcggt cgcggcgtcg cgctgcaggc ggatcgcgtc ggtgcccacg tcgccgcgcg
      421 cgtggcactt gatcgcgccc agccgcaggc acagcacctg ccacgcgaaa tcgtcgcgcg
      481 tgaggctcgt ctcgatcttg cgcaggccgc cgcgctggcc gagcacgagc gcacccaggc
      541 ggcgcagctg gctttgcgag aagcccggcg catcgacgtg gtcgagcacg taggcgctgt
      601 ggcggtggtg gtcgtggtgc gagatcatca tgccgatctc gtgcagcgcg caggcccaca
      661 gcagctcgcg ccgcatctcg gcgccggcgc cgggctgcac gctcgcgtac agcgcctgcg
      721 cgacgccgct cacacgcgcc gcctgctggg tgtcgaccat gaagcggcgc tgcagctcgc
      781 gcaccgaggc gtcgcggatg tcgtggccgt cctggcgctg ctcagcggcc ttgctgcgct
      841 cgtcgaggtc gaagatcacg ccctgacgca gcgcgccgcg cgccggcaac agcgcctcga
      901 tgccgaagtt ggcggccagc gtgtacagga tcgacaggcc gccggcgatc accgcgcgcc
      961 gctcttcctt cagcccgggc agcacgagct tgtcgacctt gccggcgcgc aggcattgct
     1021 gcatcagcca gcgcaggccc tcgggcgtga tgcggccgtc ggtgatgccg ctggcggcga
     1081 gcagctgcga gaccgcgccg accgtgcccg acgagcccag cgcctcctgc cagttcaccg
     1141 cggcgaacgg ttcgagcgct tcctcgagct cggcgccggc cgcgatctgc gcctgtcgga
     1201 acgcggcctc ggtgaagcgg ccgtcgccga agtagcgcat cgacaggctc gcgctgccga
     1261 cctggaacga ttcggcgcgc agcggccgcg agccgtggcc gaggatcatc tcggtcgagc
     1321 gcccgccgat gtcgaccacc agccgcggca ccgccgaggg ctgcaaccgg gcgacgccgg
     1381 cgtagatcag gcgcgcctct tcgcggcccg agatcacctc gatcggaaag cccagcgcgc
     1441 cctgcgcgcg gatcaggaac gcatcgcggt tgcgcgcctc gcgcagggtc tgcgtcgcca
     1501 cggcccgcac ctgggcgggc gtgaagccct tcaggcgctg cgcgaagcgc gccagacagg
     1561 ccaggccgcg cgcgccggcc gcgtcggtga gcaggccgtt cgcgtccagg ccggcgccca
     1621 ggcgcacggt ctctttctgg tagtcgatgc ggcggtagcg gccgtcgata agctggccga
     1681 tctcgagccg gaagctgttc gagcccatgt cgatcgcggc cagctggccg ggccagcgtg
     1741 ggccctcggc cgggccgggg cgggaggggg attcaggcat gccgcattgt ccgtgcaaag
     1801 cacggagttc ttcgtgacgc cgtgatcttc ggcgtgcacg agcgcatcgc cgacatcgcg
     1861 cggcgctgcg ccggcctcgg ctgcctcgcg gcgccgggtg ccccgtgccc tcagcgcacg
     1921 accaacaccg gcaccttcga gtgcgtcagc accttctgcg tctcgctgcc cagcagcagc
     1981 gccgagacgc cgcgccggcc gtgcgaggcc atcacgagca ggtcgcattc gttgcgctgg
     2041 gcgtgctcga tgatcgcctc ccaggggtgc agcgcctcga cggtgtgtga ctggcacggc
     2101 acgccggcct tctgcgccag gtcgagcacc gcctgcacgc gtttcgcggc gatgcgctcc
     2161 tgcgcgtcga agaactcctg cggcggcgtc ggctgcatct cggagatcgc gctgtacggg
     2221 aacggctctt tgacgctgat cgtgtagagc tgcgcgctca acgagcgcgc aagctcgatg
     2281 gccgcggcga tggctttcac cgtgatgtcg gaaccgtcgg tcggaacgag gatgcgcttg
     2341 aacatggtca tctccttcgc cctcccttcg caaaacggaa ggcagtggtg cgagatcatg
     2401 ctactgcgag ggttcccgcg ccagcgccgt gcaggcgccc tttgacgcag ggcaagaagc
     2461 tgccccgtgc cggtcagccg ctgcgcgcca ccggccgggc cgggtcgctg gcccattcgc
     2521 tccacgagcc tgggtagagc agcgagccgg tcagcccggc gtgctccatc gccagcaggt
     2581 tgtggcaggc ggtcacgccc gagccgcact ggtgcacggt gtcggcggcg gtttgcgtgc
     2641 cgagcaggcc ggtgaaatcg gcgcgcagct gcgctgccgg cttgaagcgg ccatcggcgg
     2701 cgaggttgtc cttgaagaag cggttcagcg cgccgggaat gtggcccgcg gtcgcatcca
     2761 gcggttcgac ctcgccgcga aagcgctcgc cggcgcgcgc gtcgagcagg cgcacgcggc
     2821 cgagcgcggc gagcagcgcg tcggcgtcca gcgtgcccac cagcgacgcg tgctcggggt
     2881 agggcgccag cgccgcgcgc atcggtgtgt cgtcggtgga cagggcgccg ccgccggcgg
     2941 tccaggccgc gacgccgccg tcgagcacgg ccgcggccgc gtggcccatc cagcgcagca
     3001 tccaccacag ccgcgcagcg tagacaccac cctgccggtc gaacgcgacg acctgggtcg
     3061 acggcgtgac gccgcagcgg cccagggtcg ctgcgaacgc ggcgcgcgtc ggcaacgggt
     3121 ggcggccatt gcggccggtc ttcgcgccgc tcaggtcgcg gtccatgtgc aggtagatcg
     3181 cgccgggcag gtggccggcg cgccagctcg cctcgccggc atcgacgtcg gcgaggtcga
     3241 agctggtgtc gatgagcagc ggcgcgttcg cgccggcgag cgcggcgcgc aactcgtcga
     3301 tggtgatcaa ggtcgtgtac ggcatgtcag gactccagcg tcgagttgac ggcgtcggcg
     3361 gcggccgcgg tgcggctgcg cagtcgggtg gcggccaggc ctgcgccgac gatcagcagc
     3421 atgcccgcga acgccatcca tgtcatccgg tcggcgaaca gcagcacgcc gtagccgaac
     3481 gagaacgcga tgcccaggta ctgcaggctc gcgatcacca gcgtgcggcc ggtgctgtag
     3541 gcgcgcgtca tcagcaactg cgcgacggtc gccagcagcc cgacggcgag cagcagcatc
     3601 gcgcccttca acgagtgctg gtggattccg ctgtacagcg tcgtgatcag gccgaagatc
     3661 gcgccgccga gcgagaagta gaagacgatg cgcatctcgg gttcgccggc gcgcgccagc
     3721 gcggtgacct gcagg
//
LOCUS       DATMJB010000249         3086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_9954, whole genome shotgun sequence.
ACCESSION   DATMJB010000249 DATMJB010000000
VERSION     DATMJB010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9954"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VNU48_05505"
     CDS             complement(<1..263)
                     /locus_tag="VNU48_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10766.1"
                     /translation="MRILNALLLGCCVALPVGAADGSSVASFSGAAPGDAPAPWKFAT
                     LPNKGPTKFTIVELEGVKVLKVDSENAYGNLVHPLKAEVTDKAV"
     gene            464..1189
                     /locus_tag="VNU48_05510"
     CDS             464..1189
                     /locus_tag="VNU48_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWI10767.1"
                     /translation="MIDTLIDELHRYAQAALESPSEMFAVLSVVVAAAFVLVSSFVKT
                     MIPLRWLAIGSNVGFVAYGALHPSYPMLLLHLALLPINFFRLAEMKRLTRRVQAVRVE
                     GEMSGVWLRPYMRSRKMRAGTVLFKKGDRGDRLYLLADGRIDLVEIGTSIAPGTIFGE
                     IAFFSPDRRRQLTARCGVNSTVLSIDEKTVSELYYQNPAFGFKMVELVAANFLADVKR
                     LNARVAELAAERDQLAARLAATD"
     gene            complement(1156..1590)
                     /locus_tag="VNU48_05515"
     CDS             complement(1156..1590)
                     /locus_tag="VNU48_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10768.1"
                     /translation="MKPGVASLAVVTLMLAAGAFAATPKPMSLDDSANFLMDSATAER
                     IWKDNTPARVSKLYPLRKFRFVSEVTGGFTDNKTCVVTARAMLLPVVLLPVQGTKVVY
                     APIKSATAFDALPSLSREQCQDLAKVKLKEAVQSVAASLAAS"
     gene            complement(1587..2999)
                     /locus_tag="VNU48_05520"
     CDS             complement(1587..2999)
                     /locus_tag="VNU48_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI10769.1"
                     /translation="MNSIRVRVLLSLLGMLVVTALVMGALTYRNVLAETESLFDYQLR
                     QMAFSLRDQGEIAPAQASTLADEELDFVVQIWTEDGRIIYPPRLRNDLPTRAVLGLAD
                     VTVQGKTWRAFSVASRDRVIQVAQPLQIRRKLAAGAALRSVAPLLFIAPAMALLIWWL
                     AAQTLAPLRRLADGVRARDEQSLQPLPTAGLPDEVAPLVGALNGLLLRLGSALGAQRA
                     FVSDAAHELRSPLTALKLQMQLLRRAPDDAARSAAAEALNAGIDRAARLVEQLLTLAR
                     TEPGAPLAALAPVELGEVVRAAVAEVVPFAHARGTAVELVAGAPVWVHGDCATLTVLA
                     RNLADNAVRYAPPRSRVELHVSEDGGAALLRVDDAGPGIPPEERARVFDRFYRHAAGD
                     ETGTGLGLAIVRSIAERHGATVRLADSPLGGLRVEVRFASAVPNKMAPGRPALPTLPG
                     AASSAAASSSPQDTTSRGDS"
BASE COUNT          457 a         1123 c         1059 g          447 t
ORIGIN      
        1 acggccttgt ccgtcacctc ggccttcagc gggtgcacca ggttgccgta ggcgttctcg
       61 gagtcgacct tcagcacctt gacgccctcg agttcgacga tcgtgaactt cgtcggcccc
      121 ttgttcggca gcgtcgcgaa cttccacggc gccggtgcgt cgcccggcgc cgcgcccgag
      181 aacgatgcga cgctcgagcc gtcggcggcc cccaccggca gcgcgacaca gcagcccagc
      241 agcaacgcat tcaggattcg catcggatcg tctccttggt cgtcgggacg acactcacgg
      301 tcgcgcctga ccgcggttgt aaccgaaaag ccatgggcgc ggcgcaacgg tcggcgcgag
      361 aaaatcgccg ccgcgcgcac cggccccaaa agcagtgctc aaattgccgc tgcccgccga
      421 cgcgcgccgt gcggccgatg cgccctctac gaaagacccg cttgtgatcg ataccctgat
      481 tgacgaactc caccgctacg cgcaggcggc cctcgagtcg ccgagcgaga tgttcgcggt
      541 gctgtcggtg gtcgtcgccg ccgcgttcgt gctggtgagc agcttcgtga agacgatgat
      601 cccgctgcgc tggctggcga tcggcagcaa cgtcggcttc gtcgcctacg gcgcgctgca
      661 tccctcgtac ccgatgctgc tgctgcacct ggcgctgctg ccgatcaact tcttccgcct
      721 ggccgagatg aagcggctga cgcgccgcgt ccaggcggtg cgggtcgagg gcgagatgtc
      781 gggtgtctgg ctgcggccct acatgcgcag ccgcaagatg cgcgccggca cggtgctgtt
      841 caagaagggc gaccgcggcg accggctcta tctgctggcc gatggccgga tcgacctggt
      901 cgagatcggc accagcatcg cgccgggcac gatcttcggc gagatcgcgt tcttctcgcc
      961 ggaccggcgc cgccagctga ccgcgcgctg cggcgtcaac agcaccgtgc tgtcgatcga
     1021 cgagaagacg gtcagcgagc tctactacca gaatccggcc ttcggcttca agatggtcga
     1081 gctggtcgct gcgaacttcc tggccgacgt caagcgcctg aacgcacgcg tggcggaact
     1141 tgccgccgaa cgcgatcagc tcgccgccag gctcgcggcg accgactgaa ccgcctcctt
     1201 cagcttgacc ttggccaggt cctggcactg ctcgcggctc aggctcggca gcgcatcgaa
     1261 ggccgtcgcc gacttgatcg gcgcgtacac gaccttggtg ccctgcaccg gcagcagcac
     1321 caccggcagc agcatcgcgc gcgccgtgac gacgcaggtc ttgttgtcgg tgaacccacc
     1381 ggtcacttcg ctgacgaagc ggaacttgcg cagcgggtac agcttgctga cgcgcgccgg
     1441 cgtgttgtct ttccagatcc tctcggcggt ggcggagtcc atcaggaagt tcgccgaatc
     1501 gtccaggctc atcggcttcg gggtggcggc gaacgcgccc gccgccagca tcagggtcac
     1561 aaccgccaga ctcgcaacgc caggtttcat gaatcgcctc gggaagtggt gtcttgtgga
     1621 gaagaagatg cggcggcgct cgacgccgcg cccggtagtg taggcagggc cggcctgccg
     1681 ggtgccattt tgttgggcac cgccgacgcg aaccgcacct ccacccgcag cccgccgagc
     1741 ggcgagtcgg cgagccgcac cgtggcgccg tgccgctcgg cgatgctgcg cacgatcgcc
     1801 agccccaggc cggtgccggt ctcgtcgccc gccgcgtggc ggtagaagcg atcgaacacg
     1861 cgcgcccgct cctcgggcgg gatgcccggc ccggcgtcgt cgacgcgcag cagcgccgcg
     1921 ccgccgtcct cggacacatg cagctcgacc cgcgaccggg gcggcgcgta gcgcaccgcg
     1981 ttgtcggcca ggttgcgtgc cagcaccgtc agcgtcgcgc aatcgccgtg cacccagacc
     2041 ggcgcgccgg cgacgagttc gaccgcggtg ccgcgcgcat gcgcgaacgg caccacttcg
     2101 gcgacggcag cgcgcacgac ttcgccgagc tccaccggcg cgagcgcggc gagcggcgcc
     2161 cccggctcgg tgcgcgccag cgtcagcagc tgctcgacca ggcgcgcggc gcgatcgatg
     2221 ccggcattca gcgcttcggc cgccgcgctg cgcgccgcgt cgtcgggggc gcgcctgagc
     2281 agctgcatct gcagcttcag cgccgtcagc ggcgagcgca gttcgtgtgc ggcatcggag
     2341 acgaacgcgc gctgcgcgcc gagcgcgctg cccaggcgca gcagcaggcc gttgagcgca
     2401 cccaccagcg gcgcgacctc gtccggcagc cccgcggtcg gcagcggctg cagcgactgc
     2461 tcgtcgcgcg cacgcacgcc gtcggccagg cgtcgcagcg gcgcgagcgt ctgcgccgcc
     2521 aaccaccaga tcagcagcgc catcgccggc gcgatgaaca gcagcggcgc gacgctgcgc
     2581 agcgccgcgc cggccgcgag cttgcgccgg atctgcagcg gctgcgcgac ctggatcacg
     2641 cggtcgcgcg aggcaacgct gaacgcgcgc caggtcttgc cctgcaccgt cacgtcggcc
     2701 aggcccagca ccgcgcgggt cggcaggtcg ttgcgcaggc gcggcgggta gatgatgcgg
     2761 ccgtcttcgg tccagatctg gaccacgaaa tcgagttcct cgtcggccag cgtgctggcc
     2821 tgcgccggcg cgatctcacc ctggtcgcgc agcgagaacg ccatctggcg cagctggtag
     2881 tcgaacagcg attcggtctc ggcgagcacg ttgcgatagg tcagcgcgcc catcaccagc
     2941 gccgtgacca cgagcatgcc cagcagcgac aggagcacgc gcacgcggat cgagttcatc
     3001 gcgggccgag cgccgggccg ctcccaaggg ccacgaagtg gcccacttcg tggcccatgc
     3061 ggcccgcatc ccctcggggg atcggc
//
LOCUS       DATMJB010000250         8704 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_619209, whole genome shotgun sequence.
ACCESSION   DATMJB010000250 DATMJB010000000
VERSION     DATMJB010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8704
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619209"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2036)
                     /locus_tag="VNU48_05525"
     CDS             complement(<1..2036)
                     /locus_tag="VNU48_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HWI10770.1"
                     /translation="MKTGVLDARPDAAPIVQLFDEHPDAAAEAAQLARARAFAEPLLA
                     GQLLDTGEEALAHADAVAAILQGIGAAPAMRAAAYLVYAGDYLNKPEEVIAKAFGPSH
                     ASLVVNTRKLVQLQRAAREAQLGSEARVQQTERVRKMLLAFSRDLRVVLLRLASRLQT
                     LRFFAASKTACPPALAAESLQVFAPLANRLGIWQLKWELEDLSFRFLEPAAYQRVAHL
                     LDEKRVEREQRVEAFRTRLADDLHAHGLRAEVQGRPKHLYSIWKKMQGKRLDFAQVLD
                     LRAVRVIVPDVAGCYAVLGRVHELFRPVAGEFDDYIERPKPNGYQSLHTVVLDDSGRA
                     VEVQIRTQAMHEHAEHGVAAHWAYKDAGAKGYAGVSADADEQARTAEARKAVLRQLLA
                     WERDTAAAGEAGAAVADDRIYVFTPAAAVVELPAGATPVDFAYTVHTDLGHRCRGARV
                     DGAMVPLNTPLHSGQTVDVITAKEGGPSLDWLNPELAYLKSARAKAKVRAWFNLLAQK
                     ETAARGREAVEKLLQREGKTSVKLDDLAAQLGFRGADALFEVVGKDEFSLRNIENLLR
                     PAEPLPSPDDLIALRRPSTAVPKGGVLVVGVESLLTSLARCCRPAPPDAISGYVTRGK
                     GVAIHRRACTNFRHMAQQSPDRVIEVQWGDGHGAVYPLDVLIEANDRQGLLRDIS"
     gene            complement(2033..2917)
                     /locus_tag="VNU48_05530"
     CDS             complement(2033..2917)
                     /locus_tag="VNU48_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI10771.1"
                     /translation="MRSDQPLTEPRLNHVQCLGGRGLHRMAYWEWGDPANPRVLLCAH
                     GISRQGRDFDTLARALSADYRVVCPDVAGRGRSDWLADPAGYQIPAYVADMVTLLARL
                     DAQTVHWVGTSMGGLIGLGLASLPQSPIERLVLNDVGPTIRFEALQRIGSYIGQPLRW
                     NTLDEAAAYLLSISAGFGPHTHEQWLALTRPMLKPDGAGFVLHYDPSIAQPFKAITPE
                     IAAAAEAMTWAAFDAIRCPTLVLRGTESDVLARDTAQAMTQRGPKARLHEFAGIGHAP
                     TLVADDQVAVVREFLLGP"
     gene            complement(2981..3739)
                     /locus_tag="VNU48_05535"
     CDS             complement(2981..3739)
                     /locus_tag="VNU48_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829174.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWI10772.1"
                     /translation="MPVAFRSPARGHRAALFAAVVSVAAPLSVLAAASPAEIASAFAP
                     QGVVSLSSSASIEVTKDLLSITFSTTREGLDAASVQAQLKQALDAALAEAKKAARPGQ
                     LDVQTGNFSLSPRYTNKGIVSGWQGSTELIVEGRDMQAIGQLSGRITTLTINRVGYGM
                     SRELREKSEGEVTVQAIARYRAKAADCARQFGYAGYAIREVNVASNEQSPMPRPMLMQ
                     ARAAAAPAPEDALAVEPGKGTVTVSVNGTVQMTK"
     gene            3901..4635
                     /gene="ompR"
                     /locus_tag="VNU48_05540"
     CDS             3901..4635
                     /gene="ompR"
                     /locus_tag="VNU48_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HWI10773.1"
                     /translation="MTTAANPRPDRIVVVDDDARIRDLLRRYLTQEGFEVLLAEDAKA
                     LNRLLTRETVHLIVLDLMLPGEDGLSICRRLRAANDLTPIIMLTAKVEDVDRIVGLEV
                     GADDYLPKPFNPRELLARIHAVLRRRPTLEAPGAPALDAQTVTFGPFEFDLALRRLSK
                     EGEQIALTTGEFSMLKALVRHPRQPLSRDKLAQLARGREFEPFDRSLDVQISRLRKMI
                     EPDPAQPRYIQTVWGVGYVFVPDGTS"
     gene            4699..6024
                     /locus_tag="VNU48_05545"
     CDS             4699..6024
                     /locus_tag="VNU48_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI10774.1"
                     /translation="MPNKLALSLFWRTFVLIALLLVSGVFAWTQTFRALEFEPRATQQ
                     AQQIAGLVKLARAALLSSDGINRVALIKGISNQEALKLAPREPGDQWEPYEVDRVSRA
                     IGHELRGQLGPDTIVASSVNGAAGLWVGFSIEKDPFWLQADPTRVGPISWSTLTLWVS
                     MALLATVLGSAAIARLINQPLKELSFAASRIREGEFDSRLDENTLTSEIRQVNMGFNR
                     MARELAKVEQDRAVMLAGISHDLRTPLARLRLEAEMSVHDDEAKQNMALDIDQLDAII
                     DKFMDYARPGDSQLTAVHLSSVVDREMAAFRDTSQIRISSRVAIDTKVMADDVELGRV
                     LQNLFENARHYGRSNDTGIARVQVTYARTGPWVILTVRDFGQGADPLKLAQLTTPFFR
                     GDAARTAATGAGLGLAIVEKAVQRMGGSFELVNAPDGGLMAHIRLKKAP"
     gene            complement(6035..7456)
                     /locus_tag="VNU48_05550"
     CDS             complement(6035..7456)
                     /locus_tag="VNU48_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10775.1"
                     /translation="MMLLTTMAALASVAIVTQDRVALRAAPRDSATQQAVLWQGDALE
                     VRGERLGYLQVYDHRRERAGYVLATQVRGSSLAPEEAPELLAVLRFVRDTPGAEALGI
                     GYAAAYLKAASAGTITAEPFDALGTMAERLARRASVRQGKAGDAVASHLEVASFYGVQ
                     FKSYERDGALRLCYDGDAFRHVFALAASAEQRARAALALTRHDCVDPALRPLERAQLD
                     AWRGDVLDRIDAAQWQQLPEALKNRLRLRRAGVWSALAFERTRQGEVAPASAAQRAID
                     ELAAVNKSELSDDDAVDYNDAAVRVGAVRWAAQTVPATPARGLRIATAPGQPGETCVM
                     LIDAAHDAAAPLAKRCSYAVVWAASARAHPNGRALALAVQPLAGWRELWVFRKQPGGW
                     VVDMLPPGTGEPELGYVEFAGWAPGGAKLLLAREARVDGRYRRSFEVASLATLNTERQ
                     AGTPALFGNWADVGWKRSTLSLR"
     gene            complement(7453..8697)
                     /locus_tag="VNU48_05555"
     CDS             complement(7453..8697)
                     /locus_tag="VNU48_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HWI10776.1"
                     /translation="MKERLTALTAAIRGAFARRLPALRHGAASSGRALLNSRSVIITT
                     MVGAVAAYALITHSPLRTVPRGDVGVRTNQLTGGVTEFREGSVLVLPALHELRSYSLR
                     DQVYHPVPGRRADGDAAFQSVEGLSLGVDLSVRYALDAARIAAIAKNLPDDIGGEVVQ
                     PAVQGVIYKVFTRYTVREIFSSKRAEIQQAIEAELRGKLAADGIVLRSVQIGKVDLPA
                     DYRRGMDRLLAEELESEKMRYTLELKEKQVAETELVAKADKVRRETAANAAASEQVIA
                     ARAQEEAMKHVLPFKQKQIEQRQFEAEAEKVARIKNAEGSAQSRRIEAQGEADSRQKL
                     ADAEAYRMDKIGKLASEQMARDGELLSRHPLLIQKTVADKLSDKIQVIIAPPGANGQF
                     IAAGLVGGATQSAAVAAPKEAE"
BASE COUNT         1202 a         3147 c         3053 g         1302 t
ORIGIN      
        1 gagatgtcgc gcagcaggcc ctggcggtca ttcgcctcga tcaacacgtc gagcgggtag
       61 acggcgccgt ggccgtcgcc ccactgcacc tcgatcacgc ggtcggggct ctgctgcgcc
      121 atgtggcgga agttcgtgca ggcgcggcgg tggatggcga cacccttgcc gcgcgtcacg
      181 tagcccgaga tggcgtccgg cggcgccggc cggcagcagc gcgccaggct cgtcagcagc
      241 gattcgacgc cgaccaccag caccccgccc ttcggcaccg cggtcgacgg gcggcgcagc
      301 gcgatcaggt cgtcgggcga cggcagcggc tcggccggcc gcagcaggtt ctcgatgttg
      361 cgcagcgaga actcgtcctt gccgacgacc tcgaacagcg cgtcggcgcc gcgaaagccg
      421 agctgcgccg ccaggtcgtc gagcttgacc gaggtcttgc cttcgcgctg cagcagcttc
      481 tcgaccgcct cgcggccgcg cgccgcggtc tccttctgcg ccagcaggtt gaaccaggcc
      541 cgcaccttgg ccttcgcgcg cgcgctcttc aggtaggcca gctcggggtt gagccagtcg
      601 agcgacgggc cgccttcctt ggcagtgatc acgtcgacgg tctggccgct gtgcagcggc
      661 gtgttcagcg gcaccatcgc gccgtcgacg cgcgcgccgc ggcagcgatg cccgaggtcg
      721 gtgtgcacgg tgtaggcgaa gtcgaccggc gtcgcgccgg ccggcagttc gacaaccgcc
      781 gccgcgggcg tgaagacgta gatgcggtcg tcggcgaccg cggcgccggc ctcgcccgca
      841 gcggcggtgt cgcgctccca ggccagcagc tggcgcagca cggccttgcg tgcctcggcg
      901 gtgcgcgcct gttcgtcggc atcggcgctg acaccggcgt agcccttcgc gccggcgtcc
      961 ttgtaggccc agtgcgccgc gacgccatgc tcggcgtgct cgtgcatcgc ctgggtgcgg
     1021 atctgcacct cgacggcgcg gccgctgtcg tccagcacca cggtgtgcag cgactggtag
     1081 ccgttcggct tcggccgctc gatgtagtcg tcgaactcgc ccgcgaccgg ccggaacagc
     1141 tcgtgcacgc gcccgagcac cgcgtagcag ccggcgacat ccggcacgat cacgcgcacc
     1201 gcgcgcaggt cgagcacctg cgcgaagtcc agccgcttgc cctgcatctt cttccagatg
     1261 ctgtacaggt gcttgggccg tccctgcact tcggcgcgca ggccatgcgc gtgcaggtcg
     1321 tcggccaggc gggtgcgaaa cgcctcgacg cgctgctcgc gctcgacgcg cttctcgtcg
     1381 agcaggtgcg cgacgcgctg gtaggccgcg ggctcgagga agcggaacga caggtcctcg
     1441 agctcccact tgagctgcca gatgccgagc cggttcgcca gcggggcgaa cacctgcagc
     1501 gactcggccg cgagcgccgg tgggcacgcc gtcttgctgg cggcgaagaa acgcagcgtc
     1561 tgcagccgcg acgccagccg cagcagcacg acgcgcaggt cgcgcgagaa cgccagcagc
     1621 atcttgcgca cgcgctcggt ctgctgcacg cgcgcctcgc tgccgagctg tgcctcgcgc
     1681 gccgcgcgct gcagctgcac cagcttgcgg gtgttgacga cgaggctggc atgcgagggc
     1741 ccgaaggcct tcgcgatcac ttcctcgggc ttgttcaggt agtcgccggc gtagacgagg
     1801 taggccgccg cacgcatcgc cggcgcggca ccgatgcctt gcagaatcgc ggcgacggcg
     1861 tcggcgtggg ccagcgcttc ctcgccggtg tcgagcagct ggcccgccag cagcggctcg
     1921 gcgaaggcgc gggcgcgcgc cagttgcgcc gcctcggcgg cggcatccgg atgctcgtcg
     1981 aacaactgga cgatgggcgc ggcgtcgggg cgcgcgtcca gcacaccggt cttcatggcc
     2041 cgagcaggaa ctcccgcacg accgccacct gatcgtcggc caccagcgtc ggcgcgtggc
     2101 cgatgccggc gaactcgtgc aggcgtgcct tcgggccgcg ctgcgtcatc gcctgcgcgg
     2161 tgtcacgcgc gagcacgtcg gactcggtgc cgcgcagcac cagcgtcggg cagcggatcg
     2221 catcgaaggc ggcccaggtc atcgcctcgg cggcggcagc gatctcgggc gtgatcgcct
     2281 tgaaaggctg cgcgatcgac gggtcgtagt gcagcacgaa gccggcgcca tcgggcttca
     2341 gcatcggccg cgtcaacgcc agccattgct cgtgggtgtg cggcccgaag ccggccgaga
     2401 tgctcagcag atacgcggcc gcctcgtcga gcgtgttcca gcgcagcggc tggccgatgt
     2461 agctgccgat gcgctgcagc gcctcgaagc ggatcgtcgg gccaacgtcg ttgagcacga
     2521 gccgctcgat cggcgattgc ggcaacgagg ccaggcccag gccgatcagc ccgcccatcg
     2581 acgtgccgac ccagtgcacg gtctgcgcgt ccagccgcgc gagcagcgtc accatgtcgg
     2641 ccacgtaggc cggaatctgg tagcccgccg gatcggcgag ccagtcggat cgcccgcgcc
     2701 cggccacgtc ggggcagacg acacggtagt ccgcgctcag cgcccgcgcc agcgtgtcga
     2761 agtcgcggcc ctgccgcgaa atgccgtgtg cacacagcag cacgcgcggg ttcgccggat
     2821 cgccccattc ccagtacgcc atgcggtgca ggccgcgccc gcccaggcac tggacgtggt
     2881 tcagacgcgg ttcggtcagg ggttggtcgc tgcgcatggc ggcatcgtaa ggcaaggccc
     2941 ttcccgcctg tcgggcgacg cggcgatgcc cttggcgccg ctacttcgtc atctgcacgg
     3001 tgccgttgac gctgaccgtc accgtgccct tgccgggctc gaccgccagc gcgtcctccg
     3061 gcgccggcgc cgccgcggcg cgggcctgca tcagcatcgg ccgcggcatc ggcgactgct
     3121 cgttgctggc cacgttgacc tcgcggatcg cgtagcccgc gtagccgaac tgccgggcgc
     3181 agtcggccgc cttcgcacgg tagcgcgcga tcgcctggac cgtgacctcg ccctcggact
     3241 tctcgcgcag ctcgcgcgac atcccgtagc cgacgcggtt gatcgtcagc gtcgtgatgc
     3301 ggccgctgag ctggccgatc gcctgcatgt cgcggccctc gacgatcagc tccgtgctgc
     3361 cctgccagcc gctcacgatg cccttgttgg tgtagcgcgg cgagagcgag aagttgcccg
     3421 tctgcacgtc aagctggccg ggccgcgcgg ccttcttcgc ttcggccagc gcggcgtcga
     3481 gtgcctgctt cagctgcgcc tgcacgctcg cggcgtcgag gccctcgcgc gtggtgctga
     3541 acgtgatgct cagcaggtcc ttcgtgacct cgatgctcgc gctgctggac aggctgacga
     3601 cgccttgcgg cgcgaacgcg ctggcgatct ccgcgggcga ggccgcggcg agcaccgaca
     3661 gcggagcggc caccgagacg accgcggcga acagcgccgc gcggtgccct cgggctgggg
     3721 atctgaatgc aacaggcatg ggcaaccctt tcgaaatgtc agtgcagctt atacgtgcga
     3781 ggccccttga cggggtcaaa ggcgggccgg agcgcatttg taacagctgg tcagagaccc
     3841 gaaaaacagg ccattttcgt gtccaaaatc ggccatgtta caaatttgga gctcccacgc
     3901 atgaccactg ctgccaaccc gcgccccgac cgcatcgtcg tcgtcgacga cgatgcccgc
     3961 atccgcgacc tgctgcgccg ctacctgacg caggaaggct tcgaggtgct gctggccgag
     4021 gacgcgaagg cactgaaccg actgctgacc cgcgaaaccg tgcacctgat cgtgctcgac
     4081 ctgatgctgc cgggcgagga cggcctgtcg atctgccgcc gcctgcgcgc cgccaacgac
     4141 ctcacgccga tcatcatgct gaccgccaag gtcgaggacg tggaccgcat cgtcggcctc
     4201 gaggtcggcg ccgacgacta cctgcccaag cccttcaacc cacgagagct gctcgcccgc
     4261 atccacgccg tgctgcggcg ccgcccgacg ctcgaagccc cgggcgcccc ggcgctcgac
     4321 gcgcagaccg tgaccttcgg tccgttcgag ttcgacctgg cgctgcgccg gctgtcgaag
     4381 gaaggcgagc agatcgcgct gacgaccggc gagttctcga tgctcaaggc gctggtgcgt
     4441 cacccgcgcc agccgctgag ccgcgacaag ctcgcccagc tcgcgcgcgg ccgcgagttc
     4501 gagcccttcg accgcagcct ggatgtgcag atctcgcgcc tgcgcaagat gatcgagccc
     4561 gacccggccc agccgcgcta catccagacg gtgtggggcg tgggctatgt gttcgtgccg
     4621 gacggcacga gctgatcgcg cgacctgccg agcggctcct tcgggagccg tttcctcgtc
     4681 tggtagcctg aggctccgat gcccaacaaa ctcgcgctca gcctgttctg gcgcaccttc
     4741 gtcctgatcg cgctgctgct cgtcagcggc gtgttcgcgt ggacccagac cttccgcgcg
     4801 ctcgagttcg agccgcgcgc gacccagcag gcgcagcaga tcgcgggcct ggtgaagctg
     4861 gcgcgggcgg cgctgctgtc cagcgacggc atcaaccggg tcgcgctgat caagggcatc
     4921 tcgaaccagg aggcgctcaa gctcgcgccg cgcgaacccg gcgatcagtg ggagccctac
     4981 gaggtcgacc gcgtgtcgcg cgcgatcggc cacgagttgc gcgggcagct cggccccgac
     5041 acgatcgtcg cgagctcggt caacggcgcg gccggcctgt gggtcggctt ctcgatcgag
     5101 aaggacccgt tctggctgca ggccgacccg acccgcgtcg gcccgatctc gtggagcacg
     5161 ctgacactgt gggtcagcat ggccttgctg gccaccgtgc tgggctcggc cgcgatcgcg
     5221 cggctcatca accagccgct gaaggaactg tcgttcgcgg ccagccgcat ccgcgagggc
     5281 gagttcgatt cgcgcctgga cgagaacacg ctgaccagcg agatccgcca ggtcaacatg
     5341 ggcttcaacc ggatggcgcg cgagctcgcc aaggtcgagc aggaccgcgc ggtgatgctc
     5401 gccggcatct cgcacgacct gcgcaccccc ttggcccggt tgcgtctgga ggccgagatg
     5461 agcgtgcacg acgacgaggc caagcagaac atggcgctgg acatcgacca gctcgacgcg
     5521 atcatcgaca agttcatgga ctacgcgcgc cccggcgaca gccagctgac cgcggtgcac
     5581 ctgagcagcg tcgtcgatcg cgagatggcc gcgttccgcg acaccagcca gatccgcatc
     5641 agctcgcgcg tcgcgatcga caccaaggtg atggccgacg acgtcgaact cggccgcgtg
     5701 ctgcagaacc tgttcgagaa cgcgcgccac tatgggcgct ccaacgacac cggcatcgcg
     5761 cgcgtgcagg tcacctatgc gcgcaccggg ccgtgggtga tcctgacggt gcgcgacttc
     5821 ggccagggcg ccgacccgtt gaagctggcg cagctgacga cgccgttctt ccgcggcgac
     5881 gccgcgcgca ccgccgcgac cggcgccggc ctcggcctgg cgatcgtcga gaaggcggtg
     5941 cagcgcatgg gtggcagttt cgagctggtc aacgcgcccg acggcgggct gatggcgcac
     6001 atccggctga agaaggcgcc gtgaggcgtc accgctagcg caggctcaag gtcgagcgct
     6061 tccagcccac gtcggcccag ttgccgaaca gcgccggcgt gcccgcctgc ctttcggtgt
     6121 tcagcgtcgc gaggctcgcg acctcgaagc tgcgccggta gcgaccgtcg acgcgcgcct
     6181 cgcgcgccag cagcagtttc gcgccgcccg gcgcccagcc ggcgaactcg acgtagccga
     6241 gctcgggctc gccggtgccg ggcggcagca tgtcgacgac ccagccgccc ggctgcttgc
     6301 ggaacaccca cagctcgcgc cagccggcca acggttgcac cgccagcgcg agggcgcggc
     6361 cgttcgggtg ggcgcgcgcc gacgcggccc agaccaccgc gtagctgcag cgcttcgcga
     6421 gcggcgccgc ggcgtcgtgg gccgcgtcga tcagcatgac gcaggtctcg cccggctggc
     6481 cgggcgctgt cgcgatgcgc aggccgcgcg cgggcgtggc gggcaccgtc tgcgcggccc
     6541 agcgcaccgc gccgacccgc acggccgcgt cgttgtagtc gacggcgtcg tcgtcgctca
     6601 gctcgctctt gttcaccgcg gccagctcgt cgatcgcgcg ctgcgcggcg ctcgccggcg
     6661 cgacctcgcc ctgccgcgtg cgctcgaacg cgagcgccga ccacacgccg gcgcggcgca
     6721 ggcgcaggcg gttcttcagc gcttcgggca actgctgcca ctgggctgca tcgatgcggt
     6781 cgagcacgtc accgcgccag gcgtcgagct gcgcgcgttc cagcgggcgc agcgccgggt
     6841 cgacgcagtc gtggcgcgtc agcgcgagcg cggcgcgggc gcgctgctcc gcgctggcgg
     6901 cgagcgcgaa gacgtgccgg aacgcgtcgc cgtcgtagca caggcgcagc gcgccgtcgc
     6961 gttcgtagct cttgaactgc acgccataga agctcgccac ctcgaggtgc gacgcgacgg
     7021 cgtcgccggc cttgccctgg cgcaccgacg cgcgccgcgc gaggcgctcg gccatcgttc
     7081 cgagcgcgtc gaagggctcg gcggtgatcg tgccggccga tgcggccttc aggtaggcgg
     7141 ccgcgtagcc gatgccgagc gcctcggcgc ccggtgtgtc gcgcacgaag cgcagcacgg
     7201 ccagcagctc gggtgcctct tcgggtgcca gggaactgcc gcgcacctgc gtcgcgagca
     7261 cgtagccggc acgttcgcgg cggtggtcgt agacctgcag atagcccagg cgctcgccgc
     7321 ggacctcgag cgcgtcgccc tgccacagca cggcctgttg ggtggccgag tcgcgcggcg
     7381 cggcgcgcag cgcgacccgg tcctgggtga cgatggcgac cgaggccagc gccgccatgg
     7441 tggtgagcag catcattcgg cctccttcgg tgcggccacc gcggccgact gcgtcgcgcc
     7501 gcccaccagg ccggcggcga tgaattgccc gttcgctccg ggcggcgcga tgatgacctg
     7561 gatcttgtcc gagagcttgt cggccacggt cttctggatc agcagcgggt gccggctcag
     7621 cagctcgcca tcgcgggcca tctgctcgct ggcgagcttg ccgatcttgt ccatccggta
     7681 ggcctcggca tcggccagct tctggcgcga atcggcctcg ccctgggctt cgatccggcg
     7741 cgactgcgcg ctgccttccg cgttcttgat gcgcgcgacc ttctcggcct cggcctcgaa
     7801 ctggcgctgc tcgatctgct tctgcttgaa cggcagcacg tgcttcatcg cctcttcctg
     7861 ggcgcgcgcg gcgatcacct gctcgctcgc cgcggcgttg gcggcagtct cgcggcgaac
     7921 cttgtcggcc ttggcgacga gctcggtttc ggcgacctgc ttctccttca gttcgagcgt
     7981 gtagcgcatc ttctcgctct cgagctcctc ggcgagcagc cggtccatgc cgcggcggta
     8041 gtcggccggc aggtcgacct tgccgatctg cacgctgcgc agcacgatgc cgtcggctgc
     8101 gagcttgccg cgcagttcgg cctcgatcgc ctgctggatc tcggcgcgct tgctcgagaa
     8161 gatctcgcgc accgtgtagc gcgtgaagac cttgtagatg accccctgca ccgccggctg
     8221 gacgacctcg ccgccgatgt cgtcgggcag gttcttcgcg atcgcggcaa tgcgcgcggc
     8281 atcgagcgcg tagcgcaccg acaggtcgac gcccagcgac aggccctcga ccgactggaa
     8341 cgccgcgtcg ccgtcggcgc ggcgccccgg caccgggtga tagacctgat cgcgcagcga
     8401 atagctgcgc aactcgtgca gtgccggcag taccagcacg ctgccctcgc ggaactcggt
     8461 gacaccaccg gtgagctggt tggtgcgcac gccgacatcg ccacgcggca ccgtgcgcag
     8521 cggcgagtgc gtgatcagcg cataggcggc gaccgcgccg accatcgtcg tgatgatcac
     8581 gctgcgcgag ttcagcaggg cgcgaccgct gctcgccgcg ccatggcgca gggccggcag
     8641 gcgccgcgcg aaggcgccgc ggatcgcggc ggtcagggca gtcagacgtt ctttcatttc
     8701 aggc
//
LOCUS       DATMJB010000251         2724 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_424209, whole genome shotgun sequence.
ACCESSION   DATMJB010000251 DATMJB010000000
VERSION     DATMJB010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424209"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VNU48_05560"
     CDS             <1..240
                     /locus_tag="VNU48_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWI10777.1"
                     /translation="LDLERALSLYRRFADRTQCGFGIGTNLTNDVGLAPLNIVMKLTR
                     CNDQPVAKLSDSPGKTLCDDRTFLAYLRQVFRVDS"
     gene            325..723
                     /locus_tag="VNU48_05565"
     CDS             325..723
                     /locus_tag="VNU48_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWI10778.1"
                     /translation="MASIHQEVLLDADPADVWDAVRDVGAVHTRVAPGFLTGCRMEAA
                     PLARVVSFANGMEARELIVDVDDAARRLVWSVVGGRMSHHNASMQIFAEGADRSRVVW
                     IADLLPSELAPAIAGMIEQGLAAMKQTLER"
     gene            754..1239
                     /locus_tag="VNU48_05570"
     CDS             754..1239
                     /locus_tag="VNU48_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10779.1"
                     /translation="MTPDDADTLEPLHEPTLWRDNGWTARVIKNEDDDGWAVAMTRDF
                     DPEPALVGPWTMGRDKKNPKPLDTAAFNVLVKTAAEVIRRHEQALHAQHHKNVSVTVA
                     GQRVLVTLAIVPDEDDPHALLAAQDAYGATLASQRVAPTFRLSAASAAAWIEDGYRRP
                     G"
     gene            complement(1240..2088)
                     /locus_tag="VNU48_05575"
     CDS             complement(1240..2088)
                     /locus_tag="VNU48_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HWI10780.1"
                     /translation="MKAPARLQSLIDEGLIDSVLRQLMSGKEAMVYVVRCGDETRCAK
                     VYKEATHRSFRQAVDYTENRKVKNTRQARAMAKGTRFGREAQEAAWQSAEVDALYRLA
                     AAGVRVPKPLNFFEGVLLMELVTDADGAAAPRLNDVAFTPEQARAHHATLLREVVRML
                     CAGVVHGDLSEFNILLAADGPVIIDLPQAIDAAGNNHAQHMLRRDVENLRNFFGAVSP
                     ELLATEFGAEIWALYERGVLTNETVLTGRFERQPGVVDLGGVLREIDDARAEEAARRL
                     RLQTVG"
     gene            complement(2113..>2724)
                     /locus_tag="VNU48_05580"
     CDS             complement(2113..>2724)
                     /locus_tag="VNU48_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWI10781.1"
                     /translation="GIQSTIHTGGPSIPGSGLIDKDVVLEADADIIGHINGGHTALSE
                     AHVCELCEKSSRAIELVHNGNERVAIAAAKMALQLKCPHRVILGTDGPAGSGVQPLGI
                     LRMIAMLSSLANLPAELVICFATGNTARIRNLNCGLIEVGRDADFVFMDRAQHTSGAT
                     LLESIRLGDIPGVGMVMIDGLVRCGRSRNTPPATEVPVIVGGH"
BASE COUNT          407 a          963 c          936 g          418 t
ORIGIN      
        1 ctcgacctcg aacgcgcgct ctcgctgtat cgtcgcttcg cggaccgcac gcaatgcggc
       61 ttcggcatcg gcacgaacct gaccaacgat gtgggcctcg cgccgctgaa catcgtgatg
      121 aagctgaccc gttgcaacga ccagccggtc gcgaagctgt ccgacagccc gggcaagacg
      181 ctgtgcgacg accggacctt cctggcctac ctgcgccagg tcttcagggt cgattcctga
      241 cgcgccgatg aacgtgtccg aaagcggcgc gtcgaccgtg ccgcgtgcgc gcatgctcgg
      301 gcttcgttca cagcggagac cgcaatggcc tcgatccacc aggaagtgct gctcgacgcc
      361 gaccccgccg acgtctggga cgcggtgcgc gatgtcggcg cggtgcacac gcgcgtcgcg
      421 cccggcttcc tgaccggctg ccggatggag gcagcgccgc tcgcgcgcgt cgtcagtttc
      481 gccaacggca tggaggcgcg cgagctgatc gtcgatgtcg acgacgcggc gcggcgcctg
      541 gtgtggtcgg tggtcggcgg gcggatgtcg catcacaacg cctcgatgca gattttcgcc
      601 gagggagcgg accgcagccg cgtcgtctgg atcgccgacc tgctgccgag cgagctggcg
      661 ccggcgatcg ccgggatgat cgagcagggc ctggccgcga tgaagcaaac gctcgaacgc
      721 tgagaaatcg atccacggcg agaatcgctg catatgacac ccgacgacgc cgacaccctc
      781 gaaccccttc acgaacccac cctctggcgc gacaacggct ggaccgcccg cgtgatcaag
      841 aacgaggacg acgacggctg ggccgtcgcg atgacgcgag actttgatcc cgagccggca
      901 ctcgtcggcc cgtggacgat gggtcgcgac aagaagaacc cgaagccgct cgacaccgcc
      961 gcgttcaacg tgctggtgaa gacggccgcc gaggtgatcc gccgccacga gcaggcgctg
     1021 cacgcgcagc accacaagaa cgtgagcgtg acggtcgcgg gacagcgtgt gctcgtcacg
     1081 ctggccatcg tgcccgacga ggacgacccg cacgcgctgc tggccgcgca ggacgcgtat
     1141 ggcgcgacgc tcgcgtccca gcgcgtcgcg ccgaccttcc ggctcagtgc ggcgagcgcc
     1201 gcggcctgga tcgaggacgg ctaccgccgc cccggctgat cagccgacgg tctgcagccg
     1261 cagccggcgc gccgcctctt cggcccgcgc gtcgtcgatc tcgcgcagca cgccgcccag
     1321 gtccaccacg ccgggctggc gttcgaagcg gccggtcagc accgtctcgt tggtcagcac
     1381 gccgcgctcg tacagggccc agatctcggc gccgaattcg gtggccagca gctccggcga
     1441 cactgcgccg aagaagttgc gcaggttctc gacatcccgc cgcagcatgt gctgcgcgtg
     1501 gttgttgccg gccgcgtcga tcgcctgcgg caggtcgatg atcaccggcc cgtcggcggc
     1561 gagcaggatg ttgaattccg acaggtcgcc gtgcacgacg ccggcgcaca gcatgcgcac
     1621 gacctcgcgc aacagcgtcg cgtggtgcgc gcgcgcctgc tcgggcgtga acgcgacatc
     1681 gttcaggcgc ggcgcggcgg cgccgtccgc gtcggtgacg agctccatca gcagcacgcc
     1741 ctcaaagaag ttcagcggct tgggcacgcg cacgccggcg gccgcgaggc ggtacagcgc
     1801 atcgacctcg gcgctctgcc acgccgcctc ctgcgcctcg cggccgaagc gcgtgccctt
     1861 ggccatcgcg cgggcctggc gggtgttctt gaccttgcgg ttctcggtgt agtcgacggc
     1921 ctggcggaag ctgcggtgcg tggcttcctt gtagaccttc gcgcagcgcg tctcgtcgcc
     1981 gcagcgaacg acatagacca tcgcttcctt gccgctcatc agctggcgca gcaccgagtc
     2041 gatcaggcct tcgtcgatca gcgattggag tcgtgcgggt gctttcatcg gcgcattctg
     2101 cgcgccgcgc gttcagtggc cgccgacgat gaccggcacc tcggtcgccg gcggcgtgtt
     2161 gcggctgcgc ccgcagcgca cgaggccgtc gatcatcacc atgccgacgc cgggaatgtc
     2221 gccgagccgg atgctctcga gcagcgtcgc gccgctggtg tgctgggcgc ggtccatgaa
     2281 cacgaagtcg gcgtcgcggc cgacctcgat cagcccgcag ttcagattgc ggatgcgcgc
     2341 ggtgttgccg gtggcgaagc agatcaccag ctcggccggc aggttcgcca ggctggacag
     2401 catcgcgatc atgcgcagga tgcccagcgg ctgcacgccc gacccggccg gcccgtcggt
     2461 gcccaggatc acgcggtgcg ggcacttcag ctgcagcgcc atcttcgccg cggcgatcgc
     2521 gacgcgctcg ttgccgttgt gcacgagctc gatcgcgcgc gaactcttct cgcacagctc
     2581 gcagacatgc gcctcgctca gcgcggtgtg gccgccgttg atgtggccga tgatgtcggc
     2641 gtcggcctcg agcaccacat ccttgtcgat cagccccgag ccggggatcg acggcccgcc
     2701 ggtgtggatc gtgctctgga tgcc
//
LOCUS       DATMJB010000252         4963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_488332, whole genome shotgun sequence.
ACCESSION   DATMJB010000252 DATMJB010000000
VERSION     DATMJB010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488332"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VNU48_05585"
     CDS             complement(<1..558)
                     /locus_tag="VNU48_05585"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWI10782.1"
                     /translation="MRHYDLPPLDQLEAFEASARHLSFTKAADELALTQSAVSRQIAA
                     LEAHYGLPLFRRLHRALRLTDDGQALFQTVAEVLGRLHQIGGELKRDRRAKTVVVTTT
                     PGFAGLWLIPRLAGFTATRPEVDVRISAGYAFVNLNRDGFDLGVRYQSEDASDAGAEL
                     LFGDVVLPVCSPKLLRDPARPLKTPE"
     gene            730..987
                     /locus_tag="VNU48_05590"
     CDS             730..987
                     /locus_tag="VNU48_05590"
                     /inference="COORDINATES: protein motif:HMM:NF022592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HWI10783.1"
                     /translation="MNTNDLDRAAISLRKGQLHRIHDGAGQRIEALSGALWVTIDHDL
                     RDIVVTPGEGFSIDRDGDTIVSALDDARFALLDPVVLRRTA"
     gene            complement(1004..1447)
                     /locus_tag="VNU48_05595"
     CDS             complement(1004..1447)
                     /locus_tag="VNU48_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HWI10784.1"
                     /translation="MKVLNLRCAHDHWFEGWFASDEDFGAQSERGLMACPVCDDKRIV
                     RMPSAPRLNVSTARGDAARPAPAGEIAHTRAQGRWLRAVRDMLARTEDVGDRFPEEAR
                     RIHYGETQERGIRGRASAEDANALRDEGIEVVALALPEALKNPVQ"
     gene            complement(1473..2063)
                     /locus_tag="VNU48_05600"
     CDS             complement(1473..2063)
                     /locus_tag="VNU48_05600"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWI10785.1"
                     /translation="MNAPISADAHLREESIESRQVYRGAFLDVRRDQVRLPDGAQAQR
                     EYIVHPGAVVIVPLLDDGRLVVERQWRYPLARVMLEFPAGKLEGDEPPLQCAIRELIE
                     ETGYRAAEWARAGILHNAIAYSNEGIEVWFARGLVLGERRLDAGEFLDVTEMSAEQLD
                     GLAQRGELTDAKTLIGLLWLQNWRAGRWPLVWQPAP"
     gene            complement(2063..2362)
                     /locus_tag="VNU48_05605"
     CDS             complement(2063..2362)
                     /locus_tag="VNU48_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HWI10786.1"
                     /translation="MDSSAAVWLVLLIAFIAANLPFVNERVFIVGPARHPKVIGWRLL
                     ELVVLWGLTLAIGFALEARAGQVQPQGWEFYAALGFAFLTFAFPGFVWRYLRRRH"
     gene            complement(2402..3883)
                     /gene="nuoN"
                     /locus_tag="VNU48_05610"
     CDS             complement(2402..3883)
                     /gene="nuoN"
                     /locus_tag="VNU48_05610"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HWI10787.1"
                     /translation="MNQMNWPAVYPAIVLLAMACVVAMVDLWVTHPKRLPTYLLTQAS
                     LAVVAAMHLWAFYAGDTVYAMQRMVVADPMGHLLGLFATIAVMVSLVYAQPYVAKRDM
                     LKGELFTLSLFSLLGIDVMLSANNFLVVYLGLELMSLSLYALVAMRRDHADATEAAMK
                     YFVLGALASGFLLYGLSMMYGATGSLEISEVFKAIGTGQINKSVMILGVVFIVAGLGF
                     KLGAVPFHMWVPDVYQGAPTAITLLLGGAPKLAAFAITIRLLVEGMLGLAGDWQQMLV
                     VLSVGSMLLGNIVAIAQSNLKRMLAYSSIAQMGFMLLGLCSGVVYDNTISAGNSYASS
                     MFYIVTYVLTTLGTFGMVMLLSRPGFEAEEIGDLAGLAKRSPWFALVMTIFMFSLAGL
                     PPTVGFYAKLSVLQSLVSTNVPGFVYLAVAAVVLSLIGAYYYLRVIKVMYFDEPVDTR
                     PIVSTPSVRAVMSLNGAAVLAFGILPGGLMTLCVQAIVKALAT"
     gene            complement(3903..>4963)
                     /locus_tag="VNU48_05615"
     CDS             complement(3903..>4963)
                     /locus_tag="VNU48_05615"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWI10788.1"
                     /translation="TLIPMYLIIGVWGGPKRVYAAFKFFLYTLAGSLLMLVALIYLYY
                     KSGGSFDILQWHKLPLPMPAQVLLFFAFFAAFAVKVPMWPVHTWLPDAHVEAPTGGSV
                     VLAAIMLKLGAYGFLRFSLPIVPDASHKWAGFMMALSLIAVVYIGFVALVQRDMKKLV
                     AYSSVAHMGFVTLGFFIFNELGVSGGLVQMISHGFVSGAMFLCIGVLYDRVHSREIAA
                     YGGVVNTMPKFATFAVLFAMANCGLPGTAGFVGEWMVILGAVKTDFWLGLIAATTLIF
                     GAAYTLWMVKRVYFGAVANQHVSELADINAREFGMLALLAAAVLVMGVYPKPFTDAMH
                     VSVDNLLKQVAVSKLPAF"
BASE COUNT          900 a         1721 c         1660 g          682 t
ORIGIN      
        1 ctcgggcgtc ttcagcgggc gcgccgggtc gcgcagcagc ttcgggctgc acaccggcag
       61 cacgacatcg ccgaacagca gctcggcgcc ggcgtcgctc gcgtcctccg actggtagcg
      121 cacgcccagg tcgaagccgt cgcggttcag gttcacgaac gcatagccgg ccgagatgcg
      181 cacgtccacc tcgggccggg tcgcggtgaa gccggccagc cgcgggatca gccacagccc
      241 cgcgaagccc ggcgtggtgg tcacgaccac cgtcttcgcg cgccggtcgc gcttgagctc
      301 gccgccgatc tggtgcagcc ggccgagcac ctcggccacg gtctggaaca acgcctggcc
      361 gtcgtccgtc aggcgcagcg cgcggtgcag gcggcgaaac agcggcagcc cgtagtgcgc
      421 ctcgagcgcg gcgatctggc ggctgaccgc cgactgcgtc agcgccagtt cgtcggccgc
      481 cttcgtgaag ctcaggtggc gtgccgaggc ctcgaaggct tcgagctggt ccagcggcgg
      541 caggtcgtag tggcgcatcg gcgggctcct cgcggcgatt tggcattcta gatccgcatg
      601 cgaggcattc gaacataccg tttgagtgtc gccccagttt cgccgagact cacttccaag
      661 caccggccaa aagccggcgc ccgcgccgaa gcgcccgcat gcggctgctt catgcaaagg
      721 agaaattcga tgaacaccaa cgacctcgac cgcgccgcga tcagcctgcg caagggccaa
      781 ctccaccgca tccacgacgg cgccgggcag cgcatcgaag cgctgagcgg cgcgctgtgg
      841 gtgacgatcg accacgacct gcgcgacatc gtcgtcacgc ccggcgaagg cttcagcatc
      901 gaccgggacg gcgacacgat cgtcagcgcg ctcgacgacg cgcgcttcgc gctgctcgat
      961 ccggtcgtgc ttcggcgtac cgcctgagcc cgcgccgaac gcgctactgc accggattct
     1021 tcagggcttc gggcagcgcc agcgcgacca cctcgatacc ctcgtcgcgc agcgcgttcg
     1081 cgtcctcggc gctggcacgg ccgcggatgc cgcgctcctg ggtctcgcca tagtggatgc
     1141 ggcgcgcctc ctcggggaag cggtcgccga cgtcctcagt gcgcgcgagc atgtcgcgca
     1201 ccgcacgcag ccagcgaccc tgcgcgcgtg tgtgggcgat ctcgccggcc ggcgcgggcc
     1261 gtgccgcatc gccgcgtgcg gtcgacacgt tcaggcgcgg cgcgctgggc atgcgcacga
     1321 tgcgcttgtc atcgcagacc gggcaggcca tcaggccgcg ctcgctttgc gcgccgaagt
     1381 cctcgtcgga ggcgaaccag ccctcgaacc agtggtcgtg cgcgcagcgc aggttcagca
     1441 ccttcatcgc acgattatca ggtcgcgggc cgtcacggcg ccggctgcca gaccagcggc
     1501 cagcgcccgg cgcgccagtt ctgcaaccac agcagcccga tcagcgtctt cgcgtcggtg
     1561 agttcgccgc gctgcgccag gccatcgagc tgctcggcgc tcatctcggt cacgtcgaga
     1621 aattcgccgg catccaggcg ccgctcgccg agcacgaggc cgcgcgcgaa ccagacctcg
     1681 atgccttcgt tcgaatacgc gatcgcgttg tgcaggatgc cggcgcgcgc ccattcggcc
     1741 gcgcgatagc cggtctcctc gatcagctcg cggatcgcgc actgcaacgg cggttcgtcg
     1801 ccttcgagct tgccggccgg gaactcgagc atcacgcgcg ccagcgggta acgccactgg
     1861 cgctcgacga ccagccggcc gtcgtcgagc agcggcacga tcaccaccgc gcccgggtgc
     1921 acgatgtact cgcgctgcgc ctgggcgccg tcgggcaggc gcacctggtc gcgccgcacg
     1981 tcgaggaagg cgccgcggta gacctggcgc gactcgatcg attcctcgcg caggtgcgcg
     2041 tcggcggaga tcggcgcgtt catcaatggc ggcgcctcag gtagcgccag acgaagcccg
     2101 ggaacgcgaa ggtcaggaag gcgaaaccga gcgcggcgta gaactcccag ccctgcggct
     2161 ggacctgccc ggcacgcgcc tcgagcgcga agccgatggc cagggtcagc ccccacagca
     2221 cgaccagttc cagcagccgc cagccgatca ccttggggtg gcgtgccggg ccgacgatga
     2281 acacccgctc gttgacgaag ggcaggttcg ccgcgatgaa cgcgatcagc agcaccagcc
     2341 agacggcggc ggaggagtcc atcaacgaat gcttgggcgg gtcacgtcgc catggcggct
     2401 gtcaagtcgc cagcgccttg acgatggcct gcacgcacag cgtcatcagc ccgccgggca
     2461 ggatgccgaa ggccagcacg gccgcgccat tgagcgacat caccgcgcgc acgctgggcg
     2521 tgctgacgat gggccgggtg tcgaccggct cgtcgaagta catcaccttg atcacgcgca
     2581 ggtagtagta cgcgccgatc agcgacagca ccaccgccgc gaccgcgagg tagacgaagc
     2641 cgggcacgtt ggtcgacacc agcgactgca gcaccgacag cttggcgtag aagcccaccg
     2701 tcggcggcag cccggcgagc gagaacatga agatcgtcat caccagcgcg aaccatgggc
     2761 tgcgcttggc caggccggcg agatcgccga tctcctcggc ctcgaagccc ggacgcgaca
     2821 gcagcatgac catgccgaag gtgccgagcg tcgtcagcac gtaggtgacg atgtagaaca
     2881 tcgacgaggc gtaggaattg ccggccgaga tcgtgttgtc gtagaccacg cccgagcaca
     2941 gcccgagcag catgaagccc atctgcgcga tgctggaata ggccagcatg cgcttcaggt
     3001 tggactgcgc gatcgcgacg atgttgccca gcagcatcga gcccaccgac agcaccacca
     3061 gcatctgctg ccagtcgccc gccaggccca gcatgccctc gaccagcagg cggatcgtga
     3121 tcgcgaaggc cgcgagcttg ggcgcaccgc ccagcagcag cgtgatcgcc gtgggcgcgc
     3181 cctggtagac gtcgggcacc cacatgtgga acggcaccgc gccgagcttg aagcccaggc
     3241 ccgcgacgat gaacaccacg cccaggatca tcaccgactt gttgatctgg ccggtgccga
     3301 tggccttgaa gacctcgctg atctcgagcg aaccggtcgc gccgtacatc atcgacaggc
     3361 cgtacagcag gaacccgctg gccagcgcac cgagcacgaa gtacttcatc gcggcctcgg
     3421 tcgcgtcggc atggtcgcgg cgcatcgcca ccagcgcgta caggctcagc gacatcagct
     3481 cgaggccgag gtagaccacc aggaagttgt tcgccgacag catcacgtcg atgcccagca
     3541 gcgagaacag gctgagcgtg aacagctcgc ccttgagcat gtcgcgcttg gccacgtagg
     3601 gttgcgcata gaccagcgac accatcaccg cgatcgtcgc gaacaggccg agcaggtggc
     3661 ccatcgggtc ggcgacgacc atgcgctgca tcgcgtacac cgtgtcgccg gcatagaagg
     3721 cccacaggtg cattgccgcg acgaccgcga gcgaggcctg cgtcagcagg taggtcggca
     3781 gccgcttcgg gtgggtgacc cacaggtcga ccatcgcgac cacgcaggcc atcgccagca
     3841 gcacgatcgc ggggtagacg gcgggccagt tcatctgatt catgggttgt tcggggccgg
     3901 attcagaagg cgggcagctt gctgaccgcc acctgcttga gcaggttgtc caccgagacg
     3961 tgcatcgcgt cggtgaacgg cttcgggtac acgcccatca cgagtaccgc cgcggcgagc
     4021 agcgccagca tgccgaactc gcgggcgttg atgtcggcga gctcgctcac gtgctggttg
     4081 gccacggcgc cgaagtacac gcgcttgacc atccacagcg tgtaggccgc gccgaagatc
     4141 agcgtggtcg cggcgatcag gccgagccag aaatcggtct tgaccgcgcc gaggatcacc
     4201 atccactcgc cgacgaaacc ggccgtgccc ggcaggccgc agttggccat cgcgaacagc
     4261 accgcgaagg tcgcgaactt cggcatcgtg ttgaccacgc cgccataggc cgcgatctcg
     4321 cgcgaatgca cgcggtcgta gagcacgccg atgcacagga acatcgcgcc cgagacgaag
     4381 ccgtgcgaga tcatctgcac cagcccgccg gagacgccga gctcgttgaa gatgaagaag
     4441 cccaaggtca cgaagcccat gtgggcgacc gacgagtacg cgaccagctt cttcatgtcc
     4501 cgctgcacca gcgcgacgaa gccgatgtag acgaccgcga tcagcgacag cgccatcatg
     4561 aagccggccc acttgtgaga cgcatcgggc acgatcggca gcgagaagcg caggaagccg
     4621 taggcgccca gcttcagcat gatggccgcc agcacgaccg agccgccggt cggcgcttcg
     4681 acgtgggcat cgggcagcca ggtgtgcacc ggccacatcg gcaccttgac cgcgaaggcg
     4741 gcgaagaagg cgaagaacag cagcacctgg gccggcatgg gcagcggcag cttgtgccac
     4801 tgcaggatgt cgaagctgcc gcccgacttg tagtagaggt agatcaacgc caccagcatc
     4861 agcagcgagc cggccagcgt gtagagaaag aacttgaagg ccgcgtagac gcgcttcggg
     4921 ccgccccaga caccgatgat caggtacatc gggatcagcg tgg
//
LOCUS       DATMJB010000253         4270 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_139382, whole genome shotgun sequence.
ACCESSION   DATMJB010000253 DATMJB010000000
VERSION     DATMJB010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139382"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..797
                     /gene="def"
                     /locus_tag="VNU48_05620"
     CDS             285..797
                     /gene="def"
                     /locus_tag="VNU48_05620"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003807300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWI10789.1"
                     /translation="MAKLNILRYPDPRLHTVAKPVPQVDARIRQLVDDMLDSMYAADG
                     VGLAATQVDVHERVIVMDTSETRDDPRVLINPELVAKSDEMTFTDEGCLSVPAVYDKV
                     SRHARVTVRALGRDGRPQEFEAEGLLAVCVQHEMDHLIGKVFVEYLSQLKRDRIKTKM
                     LKKAREDERA"
     gene            823..1782
                     /gene="fmt"
                     /locus_tag="VNU48_05625"
     CDS             823..1782
                     /gene="fmt"
                     /locus_tag="VNU48_05625"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWI10790.1"
                     /translation="MKVAFAGTPEFARVALARLHAAGFALGPVLSQPDRPAGRGLKLQ
                     PSPVKAFALEHGLAVAQPRSLRPDGKFPDDAAAARAVLQATRPDVLVVAAYGLILPPW
                     VLDLPRLGCLNIHASLLPRWRGAAPIHRAIEAGDARTGITIMQMDAGLDTGDMLLVEP
                     VAIAPDDSTATLHDRLAALGGRLIVEALELAASGGLTPRPQPLEGISYAHKIDKAEAA
                     IDWRDSAVAIERRLRAFDPFPGASTQLDGEAIKCWRGAVVAGSGAPGELLAVGDAGIT
                     VACGEGALRLTELQRAGGRRLSAAQFLQGTPLVAGRVFAAPRR"
     gene            1841..2692
                     /gene="htpX"
                     /locus_tag="VNU48_05630"
     CDS             1841..2692
                     /gene="htpX"
                     /locus_tag="VNU48_05630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HWI10791.1"
                     /translation="MFNLMKTAILMAAITALFMAIGSLLGGQQGMMLALVVALGMNFF
                     SYWFSDKLVLKMYNAQEVDESSAPQFYRMVRELAQRAELPMPKVYLINEDAPNAFATG
                     RNPEHAAVAATTGILRMLSERELRGVMAHELAHVRHRDILISTVSATMAGAISMLANF
                     AMFFGGRDSEGRSTNPVVGLLVMFLAPIAASLIQMAISRAREFEADRGGAEISGEPRA
                     LASALQKIHRAAQGIPLETAERHPETAQMMIMNPLSAGGLRGLFSTHPSTEERVERLL
                     AMAPAAA"
     gene            2860..4215
                     /locus_tag="VNU48_05635"
     CDS             2860..4215
                     /locus_tag="VNU48_05635"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018422704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HWI10792.1"
                     /translation="MKTYQFPSSFLWGAATSAYQIEGSPLADGAGPSIWHRFVRTPGL
                     VKNGDTGDVACDHYKRMKDDVAMMKRLGLTAYRFSIAWARVLPQGRGAVNEGGLGFYE
                     RLVDTLLEAGIEPMATLYHWDLPAALDDRGGWLNPDVADWFADYASVMFKRLDGRVKL
                     WATLNEPWVVTDGGYLHGALAPGHKNRFEAPIASHQLMRSHGKAVQAYRALGKHQVGL
                     VVNLAPKHPASGKPEDVAATRRADAYMNRQYLDPVFLGKYPDEMTEIFGEAWPQWPAE
                     DFKLIGEPIDFVGINYYMRDMIRHDESAWPVKTAVAPQSQSTYTDTGWEVHPQGLTEI
                     LCWIKQRYGNIPQYVTENGAAFYDPPKAEGGRMADPLRLAYYRSHLRAIHDAIEQGVD
                     LRGYCAWSLMDNLEWSLGYSKRFGLVHVNFDTQERTLKDSAHFYASVIASNGAALDHN
                     E"
BASE COUNT          693 a         1499 c         1453 g          625 t
ORIGIN      
        1 cggcgaacac gagaagcgga tcgatcacga cgggtcatgg aggtgtctct caggagctgc
       61 acggctgggg tcgcccgcgc gatggggttt agtccgctga cttcgcccaa ccagccgcgg
      121 ttcggcgaaa atcctcaatc agtttcacag attctgcccc taaacccttg atatcgcaac
      181 gaaagtgacc tcggctgacg cgtcgtgtga gacctgtcac gacctgcggc gttgcgtcag
      241 ttccacagcg gcgcagccag cgccaccgaa acgaatttcc gtccatggcc aagctcaaca
      301 tccttcgcta ccccgatcct cgcctgcaca cggtggccaa gccggtgccg caggtcgatg
      361 cacgcatccg ccaactggtc gacgacatgc tggatagcat gtacgcggcc gacggcgtcg
      421 gcctcgccgc gacccaggtc gacgtgcacg agcgcgtgat cgtgatggac acctcggaga
      481 cgcgcgacga cccgcgcgtg ctgatcaacc cggagctggt ggccaagagc gacgagatga
      541 ccttcaccga cgaaggctgc ctgtcggtgc cggccgtcta cgacaaggtg tcgcgccacg
      601 cccgggtcac ggtgcgcgcg ctcggccgtg acggccggcc gcaggagttc gaggccgagg
      661 ggctgctcgc ggtgtgcgtg cagcacgaga tggatcacct gatcggcaag gtgttcgtcg
      721 agtacctgag ccagcttaag cgcgaccgca tcaagaccaa gatgctgaag aaggcgcgcg
      781 aggacgagcg cgcatgactg gcctgccgcc gggcgcgctc cgatgaaggt cgcgttcgcc
      841 ggcacaccgg agttcgcgcg cgtcgcgctg gcgcgcttgc atgcggccgg gttcgcgctc
      901 ggtccggtgc tcagccagcc cgaccgcccg gccgggcgcg gcctgaagct gcagccttca
      961 ccggtcaagg ccttcgcgct cgagcatggc ctggccgtcg cgcagccgcg cagcctgcgg
     1021 ccggacggca agttccccga cgacgccgcc gctgcgcgcg cggtcctgca ggccacgcgg
     1081 cccgacgtgc tggtggtcgc cgcctatggc ctgatcctgc cgccatgggt gctggacctg
     1141 ccgcgcctgg gctgcctgaa catccatgcg tcgctgctgc cgcgctggcg cggcgccgcg
     1201 ccgatccatc gcgcgatcga ggccggcgac gcgcgcaccg gcatcacgat catgcagatg
     1261 gacgccggcc tggacaccgg cgacatgttg ctcgtcgagc cggtcgcgat cgcgcccgat
     1321 gacagcacgg cgacgctgca cgacaggctc gccgcgctcg gcggtcggct gatcgtcgag
     1381 gcgctcgagc tggcggccag cggcggcctg acgccgcgcc cgcagccgct cgaaggcatc
     1441 agctacgcgc acaagatcga caaggccgag gccgcgatcg actggcgcga cagcgccgtg
     1501 gccatcgaac gccggctgcg cgcgttcgac ccgtttcccg gcgccagcac gcagctcgac
     1561 ggcgaggcga tcaagtgctg gcgcggcgcg gtcgtggccg gcagcggtgc gccgggcgag
     1621 ctgctggcgg tcggcgacgc cggcatcacg gtcgcgtgcg gcgagggcgc gctgcggctg
     1681 accgaactgc agcgtgccgg cggcaggcgc ctgagcgcgg cgcagttcct acagggcacg
     1741 ccgctcgtcg ccggcagggt cttcgcggcg ccgcgccgtt gaactcgcgt acgctgcccg
     1801 catctacgcg gctgcagcgt ttcaacgagg aacccattcg atgttcaacc tgatgaagac
     1861 cgcgatcctg atggcggcca tcacggccct gttcatggcc atcggctcgc tgctcggcgg
     1921 gcagcagggc atgatgctcg cgctcgtcgt cgcgctgggc atgaacttct tcagctactg
     1981 gttttccgac aagctggtgc tgaagatgta caacgcgcag gaggtcgacg agagctcggc
     2041 gccgcagttc taccgcatgg tgcgcgagct cgcgcagcgc gcggagctgc cgatgccgaa
     2101 ggtctacctg atcaacgagg acgcgcccaa cgcgttcgcg accggccgca accccgagca
     2161 cgcggcggtc gcggccacca ccggcatcct gcgcatgctg tccgagcgcg agctgcgcgg
     2221 cgtgatggcg cacgaactcg cccacgtgcg gcatcgcgac atcctgatca gcaccgtcag
     2281 cgcgaccatg gccggagcga tctcgatgct cgcgaacttc gcgatgttct tcggcggccg
     2341 cgacagcgag ggccgctcga cgaacccggt cgtcggcctg ctggtgatgt ttctcgcgcc
     2401 gatcgcggcg agcctgatcc agatggcgat cagccgcgcg cgcgagttcg aggccgaccg
     2461 cggcggcgcc gagatctccg gcgagccgcg cgcgctggcc agcgcgctgc agaagatcca
     2521 tcgcgccgcc cagggcattc cgctcgaaac ggccgagcgc caccccgaga ccgcgcagat
     2581 gatgatcatg aacccgctgt ccgccggcgg cctgcgcggg ctgttctcga cccacccgtc
     2641 gaccgaggag cgggtcgaac ggctgctggc gatggcgcct gccgccgcct gatccggccg
     2701 ctggtcagcg cccccaagcc gccgcgaggc ggctctgctc ctgcgcttca catcgcgccg
     2761 cgaggcgcga tgattgtgga caccgcactt acccgcattg gtgcgatcgc gctggaatga
     2821 taacgtttac atgcatcgaa acatgccttg gagcagccgt tgaagaccta ccagtttccg
     2881 tcgtcctttc tttggggtgc cgccacgtcg gcctatcaga tcgagggctc gccgctggcc
     2941 gacggcgccg ggccgagcat ctggcaccgc ttcgtgcgca cgccggggct ggtgaagaac
     3001 ggcgacaccg gcgacgtcgc ctgcgaccac tacaagcgca tgaaggacga tgtcgcgatg
     3061 atgaagcgcc tgggcctgac ggcctaccgc ttcagcatcg cctgggcccg ggttctgccc
     3121 cagggccgcg gcgcggtgaa cgagggcggg ctcggcttct acgagcgcct ggtcgacacc
     3181 ttgctcgagg ccgggatcga gccgatggcc acgctctacc actgggacct tccggccgcg
     3241 ctcgacgacc gcggcggctg gctgaacccc gacgtcgccg actggttcgc cgactacgcc
     3301 agcgtgatgt tcaagcgcct ggacggccgc gtgaagctgt gggcgacgct gaacgagccc
     3361 tgggtcgtga ccgacggcgg ctacctgcac ggcgcgctcg cgcccggcca caagaaccgc
     3421 ttcgaggcgc cgatcgcctc gcaccagctg atgcgctcgc acggcaaggc cgtgcaggcg
     3481 tatcgcgcgc tcggcaagca ccaggtcggg ctggtggtga acctcgcgcc caagcacccg
     3541 gccagcggca agcccgagga cgtcgccgcg acacgccgcg ccgacgccta catgaaccgc
     3601 cagtacctcg acccggtgtt cctgggcaag taccccgacg agatgaccga gatcttcggc
     3661 gaggcctggc cacagtggcc ggcggaagac ttcaagctga tcggcgagcc catcgatttc
     3721 gtcggcatca actactacat gcgcgacatg atccgccacg acgaaagcgc gtggccggtc
     3781 aagaccgcgg tcgcgccgca gtcgcagtcg acctacaccg acaccggctg ggaagtgcac
     3841 ccgcagggcc tgaccgagat cctgtgctgg atcaagcagc gctacggcaa catcccgcag
     3901 tacgtgaccg agaacggcgc ggcgttctac gacccgccga aggccgaagg cggccgcatg
     3961 gccgatccgc tgcggctggc ctactaccgc agccatctgc gcgcgatcca cgacgcgatc
     4021 gagcagggcg tcgacctgcg gggctactgc gcctggtcgc tgatggacaa cctcgagtgg
     4081 tcgctcggct actcgaagcg cttcggcctg gtgcacgtca acttcgacac ccaggagcgc
     4141 acgctgaagg acagtgcgca cttctacgcc agcgtgatcg cgtcgaacgg cgcggcgctg
     4201 gaccacaacg agtgagctga ccgacgacac gcatggccac cacgatcaag gatgtcgcgc
     4261 gggtggccgg
//
LOCUS       DATMJB010000254         8893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_549073, whole genome shotgun sequence.
ACCESSION   DATMJB010000254 DATMJB010000000
VERSION     DATMJB010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8893
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549073"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..2131
                     /locus_tag="VNU48_05640"
     CDS             119..2131
                     /locus_tag="VNU48_05640"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI10793.1"
                     /translation="MSLAAPATPASRTSSLRIAARAPEPGHQHWSRGVALAREGRVDD
                     AAEALERAVRLAPRNALYWLNLGSVRRRQRRIDEALRCARHAFELDPRSILACHLWAE
                     LLRSQNRHAQALKVLQSLHPEVARDEQHLLLEGTLQMALGAWQDAAVTFLALLARTPA
                     HIEAYQQLGFALANLLRHADASECFRTVSMLEPQLGSVIYSAHYSAWACDWAAVADDE
                     PRLAQAMQCLGEGGGAPGFSPFCLLSLNDDAAMHRQAATVAARHLAREARGGQFDGTW
                     VAPDPGPAGYPLVVASERCRIGFVSADFRTHATSMLLVRTLERLDRSRFELTLYSHGR
                     NDDSPLRQRLHAAVDHFVECGEMSVAEQARRIRADGVAILVDMSGYTASSRLSVFALR
                     PAPVQVLWLAYPSTTGSDFIDYVIGDPVLTPLAHADDFSEKIAQLPVCYEPTDELREH
                     PDTSTRAAAGLPEGAFVYACFNQSYKITEPVFARWCRILHAVPGSVLWLLVPQAEIQN
                     ALRAQAAARGIPAERVIFAPFVTPTEHLARLPLADLFLDTFPYGAHTTCSDALWMGLP
                     VLTQVGRSFSARVAASLLAAVGLPELAVDDEGQYETLAIRLAEDRDALAAIRAHLADN
                     RLNLPLFDNARFSAELGALFARMVERWSLGRAPEHLPAIACHEVGA"
     gene            2128..3231
                     /locus_tag="VNU48_05645"
     CDS             2128..3231
                     /locus_tag="VNU48_05645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HWI10794.1"
                     /translation="MSDATPIWRFLRADNSVIAPAIRLLPDGRIEGHAHPNESSWALH
                     DGCLHFVTHDGRSSTVFDQAEGTEPGRVRLRGQFRLGGEHGVWHVLEQADARAALALG
                     VAEALEVGDRGVRLRASARVRALLTEHKIFFGRADERLSDDDEILIGRDAAIEPYAAF
                     PVGLELCTMGAFSYAESALPADLTVGRYCSIALGVDVFRDRHPMEWATTSSISYDFDA
                     RDGYRAFIAAHHEFNAARFVPTEPPGRVAPAPQIGHDVWIGQHVQLARGIRIGTGAVI
                     AAGAIVTHDVPPYAIVAGVPARVIRMRFDAALVDALLASRWWEFDASVLRRCDYQHPV
                     RFVEQVSALPDADRWHPATLNGERLLRELRALA"
     gene            complement(3250..3681)
                     /locus_tag="VNU48_05650"
     CDS             complement(3250..3681)
                     /locus_tag="VNU48_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HWI10795.1"
                     /translation="MVIGYTTGVFDLFHIGHVNLLRNAKSLCDRLIVGVTVDDLVLYK
                     NKCAVIPFDERIEIVRACSYVDLAVPQFTMDKLDAWQRYKFNVMFVGDDWYQNDRWKQ
                     FDAEFAERDVRIVYFPYTKTTSSSLINATLERLRGPAAIAA"
     gene            complement(3672..5207)
                     /locus_tag="VNU48_05655"
     CDS             complement(3672..5207)
                     /locus_tag="VNU48_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HWI10796.1"
                     /translation="MCGIFFVEQASAARIDAAAAMRLLERQRWRGPDAMQLLTLEAGH
                     VHLGHNRLAILDPTPRADQPMRSADGRYTIVFNGEIYNFRALREQHGLTTATESDTET
                     LLAGYARLGEAFFEELEGMYAFVIHDALEHRWIAARDAIGIKPLYLHRSSALTVIASE
                     PAVVAAAIAAGVDAQSVAEWRLIRRPVPGASFFLGVQELLPGTLLRSDGSSRRLWALQ
                     RRSDEFDAAGFETALREIVQQHELSDVANVGLLSGGIDSTIVMALSRTGRAYSVGLDG
                     ANEFAGAADTARRLNRELCTVGLDEATLAGHWRTLAQLRGEPLAVPNEGLIYAACRAM
                     RRDEKVVLTGEGADELLFGYDRIYRWALQTPRPDLPAFLARYGYSDTEPPTPRLLDLL
                     RGLAEGKRTIDFVEDFFFTVHLPGLLRRMDFASMAASKEARVPFADRRLVELCYRAAP
                     AHRLDANHSKLPLRRLLERLGLNGPLLRAKIGFSATPNPHLSRHAEYRGFQDLMLKEL
                     AWS"
     gene            complement(5231..6670)
                     /locus_tag="VNU48_05660"
     CDS             complement(5231..6670)
                     /locus_tag="VNU48_05660"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HWI10797.1"
                     /translation="MSAVLDDNPLAALAHELGEFRGEVAEAVDLLVSGVLELQAHRIE
                     PLEARVQSLERQASQLLGLSAAINRVSNELNLERSCRSLAEMSRMAPKSRTVVFVGRW
                     HFGDNLKYAWLAALERAQAAGYDCWYLPPDAQQEALVRSLGANCLPWTWRDWTPEHFQ
                     LAQRTAVLVIADHFFSAAYHPNPYAPALFAGANWVQLWHGISIKEVALRYPTTLHGMS
                     PFRAESLASCGRYAAFVGSSEAAQAEWRRWFAFERYGATGYPRNDVLMRAPTERDLLN
                     VDLHALEAARRTRAAGGRVVLYVPTFRDKQLGTWLHGVGLDAIADALAQRGDRLFVNL
                     HPLEHPEQAALAKRYPQALFVREHCDLYPLLREVDLMITDYSSLMFDFLPLDQPLLFY
                     RPDHATYVDDSRPLYDAKVAQLPGAVCESSAALIAALQDDPVALDAPYAPIRAELLAR
                     LFDRVDGRAAERVLALIESELERKLASQP"
     gene            complement(6741..7736)
                     /locus_tag="VNU48_05665"
     CDS             complement(6741..7736)
                     /locus_tag="VNU48_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF707 domain-containing protein"
                     /protein_id="HWI10798.1"
                     /translation="MQPYLALFRAGPSSLHPQAVERLAQQNFDYALSWFGDEAPAHAA
                     GAAFVHMQKGAKWPGLEQTLVAHWDTIQKYKYIWLPDDDLLCVPEDVSRMFAICDDLQ
                     LDLAQPALTRDSYFTHAVTLQHSAFQLRFTNFVEIMAPVLSTDMLGRIFHTLKGNISG
                     YGMDTLWPRYSQIGKVAIIDDTPVRHTRPVGGPNYLFSKKAGLAPAHEDWLCSADHFV
                     ETPADFQINLAGLLQDGNPICIGSSAEEINVVLRAVIQSCNGLQLSALQLTRYLGNHF
                     NYWAGGEFGTARYPRAMLRIVLNRTLEHTGIRFPKPPEPTGTEASPAFSVGASEQ"
     gene            complement(7860..>8893)
                     /locus_tag="VNU48_05670"
     CDS             complement(7860..>8893)
                     /locus_tag="VNU48_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HWI10799.1"
                     /translation="PDDAGVLIHRYGPHIFHTNSRQVFDHLSRFTQWRPYEHRVLAVP
                     DVRSGHGYPLPINRTTINQLYGLDLDEAGVKAFLERSREPREPVRTSEDVVLNSVGPD
                     LCDKFFRGYTRKQWGLELSELSAGVAARIPTRCNDDDRYFTDRFQAMPLHGYTAMFER
                     LLDHPGIDVRTGIAYEQIVSSADCRMTIFSGPIDEYYDHRFGRLPYRSLRFEHEHLAQ
                     QSQLQTVGTVNYPNDHDYTRITEFKHLTGQQHAGTSIVREYPTADGDPYYPIPNPANE
                     ALFKRYEALAETESDVYFIGRLAQYRYYNMDQVVAASLALSRRLNDEYAIAPVHAHYQ
                     TTASAALGV"
BASE COUNT         1237 a         3044 c         3255 g         1357 t
ORIGIN      
        1 gtgggcgacg gcgatgaggg gcggcaatcg cggccaattc cgccgatcgg cgccgtgcct
       61 gcacttctag actggccgcg cacgccggca catgccggcc cggactcgaa ggtcttccat
      121 gtcgcttgcc gcccccgcca cgcccgcttc acgtaccagt tcgctgcgca tcgccgcgcg
      181 cgctccggaa cccggccatc agcactggag ccgtggcgtc gcgctggcgc gcgaagggcg
      241 cgtggacgac gctgccgaag ccctggagcg cgccgtgcgg ctcgcgccgc gcaacgcgct
      301 gtactggctc aacctgggca gcgtgcgccg gcgccagcgc cgcatcgacg aggcgctgcg
      361 ctgcgcgcgc cacgcgttcg agctcgatcc gcgcagcatc ctggcctgcc acctgtgggc
      421 cgagctgctg cgcagccaga accgccacgc gcaggcgctg aaggtcttgc agtcgctgca
      481 ccccgaggtc gcgcgcgacg agcagcatct gctgctcgaa ggcacgctgc agatggcgct
      541 gggcgcctgg caggacgccg cggtgacctt tctcgcgctg ctcgcccgca cgcccgcgca
      601 catcgaggcc taccagcagc tcggcttcgc actggccaac ctgctgcgcc acgccgacgc
      661 ctccgagtgc ttccgcaccg tcagcatgct cgagccgcag ctcggctcgg tcatctacag
      721 cgcgcactac agcgcctggg cctgcgattg ggccgccgtc gccgacgacg aaccgcgcct
      781 ggcgcaggcg atgcagtgcc tcggcgaggg cggcggcgcg cccggcttca gcccgttctg
      841 cctgctgtcg ctgaacgacg acgcggcgat gcaccgccag gccgcgacgg tggccgcccg
      901 gcatctggcg cgcgaggcgc gcggcggcca gttcgacggc acctgggtcg cgcccgatcc
      961 gggccccgcc ggctacccgc tggtggtggc gagcgagcgc tgccgcatcg gcttcgtctc
     1021 ggccgacttc cgcacccacg ccaccagcat gctgctggtg cgcacgctcg agcggctgga
     1081 ccgctcgcgc ttcgagctca cgctgtactc gcacggccgc aacgacgaca gcccgttgcg
     1141 ccagcgcctg cacgccgcgg tggaccattt cgtcgagtgc ggcgagatgt cggtggccga
     1201 gcaggcccga cgcatccgcg ccgacggtgt cgcgatcctg gtcgacatga gcggctacac
     1261 cgcgagttcg cgcctgagcg tgttcgcgct gcggcccgcg cctgtgcagg tgctgtggct
     1321 ggcctacccg agcacgaccg gctccgactt catcgactac gtgatcggcg acccggtgct
     1381 gacgccgctg gcccatgccg acgacttcag cgagaagatc gcgcagctgc cggtgtgcta
     1441 cgagcccacc gacgagctgc gcgagcaccc cgacacctcg acgcgcgcgg ctgccggcct
     1501 gcccgagggc gcgttcgtct acgcctgctt caaccagagc tacaagatca ccgagccggt
     1561 gttcgcgcgc tggtgccgca tcctgcacgc ggtgcccggc agcgtgctgt ggctgctggt
     1621 gccgcaggcg gagatccaga acgcgctgcg cgcccaggcc gcggcgcgcg gcatcccggc
     1681 cgagcgcgtg atcttcgcgc cgttcgtgac gcccaccgag cacctggcgc ggctgccgct
     1741 ggccgatctg ttcctcgaca ccttcccgta cggcgcgcac accacctgca gcgacgcgct
     1801 gtggatgggc ctgccggtgc tgacgcaggt cggccgcagc ttctcggcgc gcgtcgccgc
     1861 gagcctgctg gccgcggtcg gcctgccgga actcgcggtc gacgacgagg gccagtacga
     1921 gacgctcgcg atccggctgg ccgaggaccg tgacgccctc gcggcgatcc gcgcgcatct
     1981 ggccgacaac cggctgaacc tgccgctgtt cgacaacgcg cgcttcagcg ccgagctggg
     2041 cgcgctgttc gcgcgcatgg tcgagcgctg gtcgctcggg cgggcgcccg agcacctgcc
     2101 ggcgatcgcc tgccacgagg tcggcgcgtg agcgacgcga cgccgatctg gcgtttcctg
     2161 cgcgccgaca attcggtgat cgcgccggcg atccggctgc tgcccgacgg gcgcatcgag
     2221 ggtcacgcgc atcccaacga gtcgagctgg gcgctgcacg acggctgcct gcacttcgtc
     2281 acgcacgacg gccgcagcag cacggtgttc gaccaggccg aaggcaccga gcccgggcgc
     2341 gtgcgcctgc gcggccagtt ccggctcggc ggcgagcacg gcgtgtggca tgtgctggaa
     2401 caggccgacg cgcgtgccgc gctcgcgctc ggcgtggccg aggcgctcga ggtcggcgac
     2461 cgcggcgtgc gcctgcgcgc gagcgcgcgc gtgcgcgcac tgctgaccga gcacaagatc
     2521 ttcttcggcc gcgccgacga gcggctcagc gacgacgacg agatcctgat cgggcgcgac
     2581 gccgcgatcg agccctatgc cgcgttcccg gtcggcctgg aactgtgcac gatgggcgcg
     2641 ttctcgtacg ccgaatcggc gctgccggcc gatctgacgg tcggacgcta ctgctcgatc
     2701 gcactcggcg tcgacgtgtt ccgggatcgc cacccgatgg agtgggccac gacctcgtcg
     2761 atcagctacg acttcgacgc gcgcgacggc taccgtgcct tcatcgccgc gcaccacgag
     2821 ttcaacgccg cccgtttcgt gccgaccgag ccgcccgggc gcgtcgcacc ggcgccgcag
     2881 atcggccacg acgtctggat cggccagcac gtgcagctcg cccgcggcat ccgcatcggc
     2941 accggcgcgg tgatcgcggc cggcgcgatc gtcacgcacg acgtgccgcc gtacgcgatc
     3001 gtcgccggcg tgccggcgcg cgtgatccgg atgcgcttcg acgcggcgct cgtcgacgcg
     3061 ctgctggcgt cgcgctggtg ggaattcgac gcctcggtgc tgcgccgctg cgactaccag
     3121 cacccggtgc gcttcgtcga acaggtgagc gcgctgcccg atgccgatcg ctggcacccg
     3181 gccacgctga acggcgagcg gctgctgcgg gaattgcgcg cgctcgcctg agcagccggc
     3241 gccttcggct caggcagcga tggccgcggg gccgcgcagg cgctccagcg tcgcgttgat
     3301 caggctcgac gaggtcgtct tcgtgtacgg gaagtagacg atgcgcacgt cgcgctcggc
     3361 gaactcggcg tcgaactgct tccagcgatc gttctggtac cagtcgtcgc cgacgaacat
     3421 cacgttgaac ttgtagcgct gccaggcgtc gagcttgtcc atcgtgaact gcggcaccgc
     3481 gaggtcgacg tagctgcagg cacgcacgat ctcgatgcgc tcgtcgaacg ggatcaccgc
     3541 gcacttgttc ttgtacagca ccaggtcgtc gacggtgacg ccgacgatca gccggtcgca
     3601 cagcgacttg gcattgcgca gcaggttcac gtggccgatg tggaacaggt cgaacacgcc
     3661 ggtggtatag cctatgacca tgcgagttcc ttcagcatca ggtcctggaa gccgcggtac
     3721 tcggcgtggc gggacaggtg ggggttcggt gtcgcgctga agccgatctt ggcgcgcaag
     3781 agcgggccgt tcaggcccag gcgttcgagc aggcggcgca gcggcagctt gctgtggttc
     3841 gcatcgagcc ggtgcgccgg cgcggcgcgg tagcacagct cgaccaggcg gcggtcggcg
     3901 aagggcacgc gcgcttcctt cgaggccgcc atcgacgcga agtccatgcg ccgcagcagg
     3961 cccggcaggt gcaccgtgaa gaagaagtcc tcgacgaagt cgatcgtgcg cttgccttcg
     4021 gccaggccgc gcagcaggtc gagcaggcgc ggcgtcggcg gctcggtgtc gctgtagccg
     4081 tagcgcgcca ggaaggcggg caggtcgggt cgcggcgtct gcagcgccca gcggtagatg
     4141 cggtcgtagc cgaacagcag ctcgtcggcg ccttcgccgg tcagcacgac cttctcgtca
     4201 cggcgcatcg cgcggcaggc cgcatagatc aagccttcgt tcggcaccgc gagcggctcg
     4261 ccgcgcagct gcgcgagcgt gcgccagtgg ccggcgagcg tcgcttcgtc gagcccgacg
     4321 gtgcacagtt cgcggttgag ccggcgcgcg gtgtcggccg cgccggcgaa ttcgttggcg
     4381 ccgtcgagtc cgaccgagta ggcgcgtccg gtgcgcgaca gtgccatcac gatcgtgctg
     4441 tcgatgccgc cgctgagcag gccgacgttg gccacgtcgc tgagctcgtg ctgctgcacg
     4501 atctcgcgca gcgcggtctc gaagccggcg gcgtcgaatt cgtcgctgcg gcgttgcagt
     4561 gcccacaggc gccggctgct gccgtcgctg cgcagcagcg tgccgggcag cagttcctgc
     4621 acgccgagga agaaactcgc gcccggcacc gggcggcgga tcagccgcca ctcggcgacc
     4681 gactgcgcat cgacgccggc cgcgatcgca gcggcgacca ccgccggctc ggaggcgatc
     4741 accgtcagcg cgctgctgcg gtgcaggtac agcggcttga tgccgatggc gtcgcgcgcc
     4801 gcgatccagc ggtgctcgag cgcgtcgtgg atcacgaacg cgtacatgcc ttcgagctct
     4861 tcgaaaaagg cctcgccgag ccgcgcgtag ccggccagca gcgtttcggt gtcggactcg
     4921 gtcgcggtcg tcaggccgtg ctgctcgcgc agtgcgcgga agttgtagat ctcgccgttg
     4981 aagacgatcg tgtagcggcc gtcggccgag cgcatcggct ggtcggcgcg cggcgtcggg
     5041 tccaggatcg cgaggcggtt gtggccgagg tggacgtggc cggcctccag cgtcagcagc
     5101 tgcatcgcgt cggggccgcg ccagcgctgg cgttcgagca gccgcatggc ggccgcggcg
     5161 tcgatgcgcg cggcgctcgc ttgctcgacg aagaagatgc cgcacatggg cgaaggccgc
     5221 gcggtccggg tcagggctgg gatgcgagct tgcgttcgag ctcgctctcg atcagcgcca
     5281 gcacgcgttc ggcggcgcgg ccgtcgaccc ggtcgaacag ccgcgcgagc agctcggcac
     5341 ggatcggcgc gtacggcgca tcgagcgcca ccgggtcgtc ctgcaaggcg gcgatcagcg
     5401 cggcgctgct ctcgcagacc gcgccgggca gctgcgcgac cttggcgtcg tacagcggcc
     5461 gcgaatcgtc gacataggtg gcgtggtcgg ggcggtaaaa cagcagcggc tggtccagcg
     5521 gcaggaagtc gaacatcagc gacgaatagt cggtgatcat caggtcgacc tcgcgcagca
     5581 gcgggtacag gtcgcagtgc tcacgcacga acagcgcctg cgggtagcgc ttcgcgagcg
     5641 ccgcctgttc ggggtgctcc agcgggtgca ggttcacgaa cagccggtcg ccgcgctgcg
     5701 ccagcgcgtc ggcgatcgcg tccaggccga cgccgtgcag ccaggtgccg agctgcttgt
     5761 cgcggaaggt cggcacatac agcaccacgc gaccgccggc ggcgcgcgtg cgacgcgcgg
     5821 cctcgagcgc gtgcaggtcg acattcagca ggtcgcgctc ggtgggcgcg cgcatcagca
     5881 cgtcgttgcg cgggtagccg gtggcgccgt agcgttcgaa cgcgaaccag cgtcgccatt
     5941 cggcctgggc cgcctcgctg ctgccgacga acgcggcgta gcgcccgcac gaggcgagcg
     6001 actcggcacg gaacggcgac atgccgtgca gggtcgtcgg gtagcgcagc gccacttcct
     6061 tgatcgagat gccgtgccag agctggaccc agttcgcccc ggcgaacagc gccggcgcgt
     6121 acggattcgg gtggtaggcg gccgagaaga agtggtcggc gatcaccagc accgcggtgc
     6181 gctgcgcgag ctggaagtgt tcgggggtcc agtcgcgcca ggtccacggc aggcagttcg
     6241 cgccgagcga tcgcaccagc gcctcctgtt gcgcgtcggg cggcaggtac cagcagtcgt
     6301 agccggccgc ctgggcgcgc tcgagcgcgg cgagccaggc gtacttcagg ttgtcgccga
     6361 agtgccagcg gccgacgaag accacggtgc gcgacttcgg cgccatgcgc gacatctcgg
     6421 ccaggctgcg gcagctgcgc tccaggttga gctcgttcga cacgcggttg atcgccgcgc
     6481 tcaggcccag cagctgcgag gcctggcgct ccagcgactg gacgcgcgct tccagcggct
     6541 cgatgcggtg ggcctgcagt tcgagcacgc ccgagaccag caagtcgacc gcttccgcga
     6601 cctcgccgcg gaactcgccg agttcgtgcg ccagcgcggc cagcgggttg tcgtcgagca
     6661 ctgcgctcat cggaccccca cgacgctgcg cgtcggcccc ccgagggggc gcgagcgcct
     6721 tgggagcggc ccggcggcgc tcattgctcg gaggcgccga cgctgaacgc gggggaggcc
     6781 tcggtgccgg tgggttccgg cggcttcggg aagcggatgc cggtgtgctc cagcgtgcgg
     6841 ttcagcacga tgcgcagcat cgcgcgcggg tagcgcgccg tgccgaactc gccgccggcc
     6901 cagtagttga aatggttgcc gaggtagcgc gtcagttgca gcgcgctgag ctgcagcccg
     6961 ttgcacgact ggatcacggc cctcagcacg acgttgatct cctcggccga gctgccgatg
     7021 cagatcgggt tgccgtcctg cagcagcccg gccaggttga tctggaagtc ggccggggtc
     7081 tcgacgaagt gatcggccga gcacagccag tcctcgtgtg ccggcgccag gccggccttc
     7141 ttgctgaaca ggtagttcgg gccgccgacc gggcgggtgt gcctgaccgg cgtgtcgtcg
     7201 atgatcgcga ccttgccgat ctggctgtag cgcggccaca gcgtgtccat gccgtagccg
     7261 ctgatgttgc ccttgagcgt gtggaagatg cgcccgagca tgtcggtgct cagcaccggc
     7321 gccatgatct cgacgaagtt cgtgaagcgc agctggaacg cgctgtgctg cagcgtgacc
     7381 gcgtgggtga agtacgagtc gcgcgtcagc gccggctgcg ccaggtcgag ctgcaggtcg
     7441 tcgcagatcg cgaacatgcg cgacacgtcc tcgggcacgc acagcaggtc gtcgtcgggc
     7501 agccagatgt acttgtactt ctggatcgtg tcccagtgcg cgaccagggt ctgctccagc
     7561 ccgggccact tggcgccctt ctgcatgtgc acgaaggccg cgccggcggc gtgcgccggg
     7621 gcctcgtcgc cgaaccacga cagcgcgtag tcgaagttct gctgcgccag gcgctcgaca
     7681 gcctgtgggt gcagcgaaga cgggccggcg cggaagaggg cgaggtaggg ttgcatgttc
     7741 ggttcctgtg ggccgtgcag gcctgaatcg gtgtgctgcc agtctaggca gcggggccgt
     7801 gaagcgaagc ggcgataagg cggagcaaac gcgtgcatct cgcacgcgct gccggagcgt
     7861 cagacgccca gcgccgccga cgcggtggtc tggtaatgag cgtgcaccgg cgcgatcgcg
     7921 tactcgtcgt tcaggcgccg gctcagcgcc agcgaggccg cgacgacctg gtccatgttg
     7981 tagtagcggt actgcgcgag gcggccgatg aagtacacgt cgctctcggt ctcggccagc
     8041 gcctcgtagc gcttgaacag cgcctcgttg gcggggttcg ggatcgggta gtacgggtcg
     8101 ccgtcggcgg tcgggtactc gcgcacgatc gacgtgccgg cgtgttgctg gccggtcagg
     8161 tgcttgaact cggtgatgcg ggtgtagtcg tggtcgttcg ggtagttcac cgtgcccacc
     8221 gtctgcagtt gcgactgctg cgccaggtgc tcgtgctcga agcgcagcga gcggtagggc
     8281 aggcgcccga agcggtggtc gtagtactcg tcgatcgggc cgctgaagat cgtcatgcgg
     8341 cagtccgcgc tcgagacgat ctgctcgtag gcgatgccgg tgcgcacgtc gatgccgggg
     8401 tggtcgagca gccgctcgaa catcgcggtg tagccgtgca gcggcatggc ctggaagcgg
     8461 tcggtgaaat agcggtcgtc gtcgttgcag cgcgtcggga tgcgcgcggc cacgccggcg
     8521 ctgagctcgg acagctccag cccccattgc ttgcgcgtgt agccgcggaa gaacttgtcg
     8581 cacaggtccg ggccgaccga gttgagcacc acgtcttccg aggtgcgaac gggctcgcgc
     8641 ggctcgcgcg agcgctcgag gaaggccttg acgccggctt cgtccaggtc caggccgtag
     8701 agctggttga tggtcgtgcg gttgatcggc agcgggtagc cgtggccgct gcgcacgtcg
     8761 ggtaccgcca gcacgcggtg ctcgtagggg cgccactggg tgaagcgcga caggtggtcg
     8821 aagacctgcc tggagttggt gtggaagatg tgcgggccgt agcggtggat cagcacgccg
     8881 gcgtcgtccg ggt
//
LOCUS       DATMJB010000255         4422 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_458780, whole genome shotgun sequence.
ACCESSION   DATMJB010000255 DATMJB010000000
VERSION     DATMJB010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458780"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1505
                     /locus_tag="VNU48_05675"
     CDS             225..1505
                     /locus_tag="VNU48_05675"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWI10800.1"
                     /translation="MTPTDLPAMIAHLGAAARAASARMAAAPTAAKNAALLALARRLR
                     ATPEALRAANALDIEAATSAGLAAPMIDRLKLTPGIIETVAQGCEQIAAMADPIGEIS
                     GLAQRPSGIQVGRMRVPLGVFGMIYESRPNVTIEAASLAIKSGNAAILRGGSEAIHSN
                     LALWQLVQAALEDAGLPPDAVQLVQTTDRAAVGLLIASPEAVDVIIPRGGKGLIERIA
                     REAKVPVIKHLDGNCHVFVDASADLELALRVTDNAKTQKYSPCNAAESLLVHAQIAPA
                     FLPRIGALFAAKGVEMRGDAAALALLQGVAGAKLAAASEQDWAEEYLAPIISVKVVAD
                     LDAAIAHINRYGSHHTDAILTDTHTHAMRFLREVDSASVMVNASTRFADGFEYGLGAE
                     IGISTDKFHARGPVGLEGLTSLKWIVLGHGEVRG"
     gene            1510..2208
                     /gene="queC"
                     /locus_tag="VNU48_05680"
     CDS             1510..2208
                     /gene="queC"
                     /locus_tag="VNU48_05680"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HWI10801.1"
                     /translation="MDPRRALVLFSGGQDSTASLAWALERYAQVETVGFDYGQRHRVE
                     LDCRPRVLAALRADFPHWAGRLGDDHLLDLALLGRISDTALTDARAIEFDARGLPNTF
                     VPGRNLLFFTLAAALAYRRGLSVLVGGMCETDFSGYPDCRDNTLKALQVAISLGLDAP
                     MTIETPLMWLAKAQTWALTEQLGGAALVDLTIEHTHTCYLGDRSQRHAWGYGCGACPA
                     CELRRKGYERWLSG"
     gene            2283..2810
                     /locus_tag="VNU48_05685"
     CDS             2283..2810
                     /locus_tag="VNU48_05685"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWI10802.1"
                     /translation="MSTSQIVGWAIAALLLFWAVGAYNRLVRLRSAIVRQFAPVDEQF
                     RRRHALLLQLLDALAPLLANAQSRIEILRAACQQVAAACAHAKQRPGAAGAITSLRLA
                     DAILAEARARLPVPSAPGSELAALNAQLTEADAMLAFARRQFDDAVLDYNRAVRQFPT
                     LLLVGLFGFRSAATL"
     gene            2865..3197
                     /locus_tag="VNU48_05690"
     CDS             2865..3197
                     /locus_tag="VNU48_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010812125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWI10803.1"
                     /translation="MPPTLRPLLLGAALIAAGPAALAQDAATLRARSLAASCAQCHGT
                     DGHTPPGSAMRALAGLAAPYLTEQLLAFRSGERPGSVMPQLAKGYSEAQITQLAAYFA
                     GVPAAATP"
     gene            3206..>4422
                     /locus_tag="VNU48_05695"
     CDS             3206..>4422
                     /locus_tag="VNU48_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HWI10804.1"
                     /translation="MHRRRLLKLAGAAGLGLSGCATTPALNTIGTAGRARVLVVGGGY
                     GGATAAKYLRLLSKDKIDVVLVEADAAFVSCPMSNLVLGGYRAMTDITRPYDLLGARH
                     GVRVVRDAVASIDATQRVAVLAGGATIRYDKLVLSPGVEMIWDAVAGLRAASQRGRLL
                     QAWEAGPETVALRRQLEAMPNGGVFAIAIPEAPYRCPPGPYERASLVAAYFKAAKPRS
                     KVLILDANEDVTSKGALFKKAWAESYKGLLEYQPQSKAIGVDAATNTVKFEIQDDVRA
                     DVLNVLPPMRAGAIAVQTGLANSNARWCNVHWLNFESTVAKHIHVIGDSIQIASQMPK
                     SGHMANSQAKVAAAAIVAELNGWELDPAPLLSNTCYSFVDDKRAVHVASIHAYTAASK
                     TFEPVAGAGGLSSA"
BASE COUNT          629 a         1616 c         1595 g          582 t
ORIGIN      
        1 cgcgccggcc gcccgccagc aggccgcagc cgccgccggc ccgcgcgccg cctgcgcggg
       61 caagaccgag ttcgcgctgt accgctgcat gcagcagcag tgcgaggcat cgcgctggta
      121 cgggcacccg cagtgcatcc ggctgcgcgc gaccgaccgc gccgaatagc gcaaccgcat
      181 ccgtgccggc ggcaggccgg cggccggttt gcgacaatcg cggcatgacc ccgaccgatc
      241 tgcccgcgat gatcgcccac ctgggcgctg ccgcccgtgc ggcctcggca cgcatggcgg
      301 ccgcgccgac cgccgcgaag aacgccgcgc tgctcgcgct cgcgcggcgc ctgcgcgcca
      361 cgcccgaggc gctgcgcgcg gcgaacgcgc tcgacatcga ggcagcgacc agcgcgggcc
      421 tggccgcccc gatgatcgat cggctgaagc tgacgcccgg gatcatcgag accgtcgcgc
      481 agggctgcga gcagatcgcc gcgatggccg acccgatcgg cgagatttcg ggcctggcgc
      541 aacgcccgag cggcatccag gtcgggcgca tgcgtgtgcc gctgggcgtg ttcggcatga
      601 tctacgagag ccggccgaac gtgacgatcg aggccgcgtc gctggcgatc aagagcggca
      661 atgcggcgat cctgcgcggc ggctcggagg cgatccattc gaacctcgcg ctgtggcagc
      721 tggtgcaggc cgcgctcgaa gatgccggcc tgccgcccga cgcggtgcag ctggtgcaga
      781 ccaccgaccg cgccgcggtc gggctgctga tcgcctcgcc ggaggcggtc gacgtgatca
      841 tcccgcgcgg cggcaagggg ctgatcgagc ggatcgcgcg cgaagccaag gtgccggtga
      901 tcaagcacct ggacggcaac tgccatgtct ttgtggacgc aagcgccgac ctcgagctcg
      961 cgctgcgcgt caccgacaac gcgaagacgc agaagtacag cccgtgcaac gcggccgagt
     1021 cgctgctggt gcacgcgcaa atcgcgccgg ccttcctgcc gcgcatcggc gcgctgttcg
     1081 ccgccaaggg cgtcgagatg cgcggcgacg ccgcggcgct ggcgctgctg cagggcgtgg
     1141 cgggcgcgaa gctggcggcg gccagcgagc aggactgggc cgaggagtac ctcgcgccga
     1201 tcatcagcgt gaaggtcgtc gccgacctcg acgcggcgat cgcgcacatc aaccgctacg
     1261 gctcgcacca caccgacgcg atcctgaccg acacgcacac gcacgcgatg cgcttcctgc
     1321 gcgaggtcga ttcggccagc gtgatggtga acgcgtcgac gcgcttcgcc gatggcttcg
     1381 agtacggcct gggcgccgag atcggcatca gcaccgacaa gttccacgcg cgcgggccgg
     1441 tcggcctcga aggcctgacc tcgctgaagt ggatcgtgct cggccatggc gaggtgcgcg
     1501 gctgagccga tggacccgcg ccgtgcgctg gtgctgttct cgggcgggca ggactcgacg
     1561 gcctcgctcg cctgggcgct ggagcgctac gcgcaggtcg agaccgtcgg cttcgactac
     1621 ggccagcgcc accgcgtcga gctcgattgc cggccgcgcg tgctcgccgc gctgcgtgca
     1681 gactttccgc actgggccgg gcggctcggc gacgaccacc tgctcgacct cgcgctgctg
     1741 ggccggatct ccgacaccgc gctgaccgat gcgcgcgcga tcgagttcga cgcgcggggc
     1801 ctgccgaaca ccttcgtgcc cgggcgcaac ctgcttttct tcaccctcgc cgcggcgctc
     1861 gcctaccggc gcgggctgag cgtgctggtc ggcggcatgt gcgagaccga cttctccggc
     1921 tacccggatt gccgcgacaa cacgctgaag gcgctgcaag tcgcgatcag cctcgggctg
     1981 gacgcgccga tgacgatcga gaccccattg atgtggctgg ccaaggcgca gacctgggcg
     2041 ctgacggagc aactcggtgg cgcggcgctg gtcgatctga cgatagagca cacccacacc
     2101 tgctacctcg gcgaccgctc gcagcgccac gcctggggtt acggctgcgg cgcgtgcccg
     2161 gcctgcgagc tgcgccgcaa aggctacgag cgctggctca gcggctgagc cgggcgcgag
     2221 tgcacgggcg tgccgaagcg ccgcgctcgc gcgcttggcg cgctgtcgta gagtgcgctc
     2281 cgatgagtac ctcgcaaatc gtcggctggg cgatcgcggc gctgctgctg ttctgggcgg
     2341 tcggcgccta caaccggctg gtgcgcctgc gcagcgcgat cgtgcgccag ttcgcgccgg
     2401 tcgacgaaca gttccggcgc cgtcacgcgc tgttgctgca gttgctggac gcgctcgcgc
     2461 cgctgctggc gaacgcgcaa tcgcgcatcg agatcctgcg cgccgcctgt cagcaggtgg
     2521 cggcggcctg cgcccacgcg aagcagcggc ccggcgcggc cggcgccatc accagcctgc
     2581 ggctggccga cgcgatcctg gccgaggcgc gcgcgcggct gccggtgccg agcgcgccgg
     2641 gcagcgagct cgccgcgctg aacgcacagc tgaccgaggc cgacgcgatg ctcgcgttcg
     2701 cgcgccgcca gttcgacgac gcggtgctcg actacaaccg cgcggtcagg cagttcccga
     2761 cgctgctgct ggtcggcctg ttcggctttc gcagcgcggc cacgctctga cgcagccgcc
     2821 ggactcgcgc gacactacgc ctcacgagcc ccggagaacc cgcgatgccg ccgaccctgc
     2881 gccccctgct gcttggcgcc gcgctgatcg cggccggccc cgccgcgctc gcgcaggacg
     2941 ccgccacgct gcgcgcgcgc agcctcgccg cgagttgcgc gcaatgccac ggcaccgacg
     3001 gccacacgcc gccggggtcg gcgatgcgag cgctggccgg cctggccgcg ccctacctga
     3061 ccgagcagct gctggcgttt cgcagcggcg agcgccccgg cagcgtgatg ccgcagctcg
     3121 cgaagggcta cagcgaggcg cagatcacgc agctggccgc gtacttcgcc ggcgtgccgg
     3181 cggccgctac cccgtgaggc ccgcgatgca tcgccgccgc ctgctcaagc tcgccggcgc
     3241 cgccggtctc ggcctcagcg gctgcgcaac gacgccggcg ctgaacacca tcggcacggc
     3301 cgggcgcgca cgcgtgctcg tcgtcggcgg cggctacggc ggcgcgaccg ccgcgaaata
     3361 cctgcgcctg ctgtcgaagg acaagatcga cgtggtgctg gtcgaggccg atgcggcgtt
     3421 cgtctcgtgc ccgatgtcga acctggtgct cggcggctac cgcgcgatga ccgacatcac
     3481 gcggccctac gacctgctgg gcgcgcgcca cggcgtgcgc gtcgtcaggg acgcggtggc
     3541 cagcatcgac gcgacgcagc gcgtcgcggt gctcgccggc ggcgccacga tccgctacga
     3601 caagctcgtg ctgtcgcccg gcgtcgagat gatctgggac gccgtggccg ggctgcgtgc
     3661 ggccagccag cggggccgcc tcctgcaggc ctgggaggcc gggcccgaaa cggtggcgct
     3721 gcgccgccag ctcgaggcga tgcccaacgg cggcgtgttc gcgatcgcga tccccgaggc
     3781 gccgtaccgc tgcccgcccg ggccgtacga gcgcgcgtcg ctggtcgccg cgtacttcaa
     3841 ggccgcgaag ccgcgctcga aggtgctgat cctcgacgcg aacgaggacg tgaccagcaa
     3901 gggcgccctc ttcaagaagg cgtgggccga gtcgtacaag ggcctgctcg agtaccagcc
     3961 gcagtcgaag gcgatcggcg tcgacgcggc cacgaacacg gtcaagttcg agatccagga
     4021 cgacgtgcgc gccgacgtgc tgaacgtgct gccgccgatg cgcgccgggg cgatcgcggt
     4081 gcagaccggc ctggcgaatt cgaacgcgcg ctggtgcaac gtgcactggc tgaacttcga
     4141 atcgaccgtg gcgaaacaca tccacgtgat cggcgactcg atccagatcg cctcgcagat
     4201 gcccaagagc ggccacatgg cgaacagcca ggcgaaggtc gcggccgcgg cgatcgtcgc
     4261 ggagctgaac ggctgggaac tcgaccccgc gccgctgctc tcgaacacct gctacagctt
     4321 cgtcgacgac aagcgcgcgg tgcacgtcgc aagcattcac gcgtacaccg cggccagcaa
     4381 gaccttcgag ccggtggccg gggcgggcgg cctgtcgagc gc
//
LOCUS       DATMJB010000256         4936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_8928, whole genome shotgun sequence.
ACCESSION   DATMJB010000256 DATMJB010000000
VERSION     DATMJB010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8928"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1035
                     /gene="flhF"
                     /locus_tag="VNU48_05700"
     CDS             <1..1035
                     /gene="flhF"
                     /locus_tag="VNU48_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HWI10805.1"
                     /translation="RDTLPVAPEPPVPALADHAHPGHAATRRDQEDMMSELRSMKGLI
                     EERFGALAFMEKLNRQPAQARLTQKLLDCGFSPALIRKIGDSLPMDVSDESAWAAGVL
                     ERNLLTAEAEAPLEDQGGVYALVGATGVGKTTSTAKLAAAFAAKYGAANLGLITLDAY
                     RIGAHEQLRAYGRILGVPVHTAHDRASLEDLLELLGGKKMVLIDTAGMAQRDTRTREL
                     LDMLSHRSIRKLLVINAASQGETIEDVLIAYQAAACAGIVLSKMDEAVKLGPALDVLI
                     RMKLKVVGVANGQRVPEDWHRLSANALVHRALRGGGSSAYRLDASDVSLIFTAPMAST
                     RAPARGALHG"
     gene            1040..1882
                     /locus_tag="VNU48_05705"
     CDS             1040..1882
                     /locus_tag="VNU48_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein"
                     /protein_id="HWI10806.1"
                     /translation="MNRFFDDDLTMSRPMPLDQADGLRRLFAHSRVCVLPVVSNPNVA
                     FGGVMLERLCTAFAEHGRHTLVVDAAERAPLHSEMALLDLAECIEPLSAQVSYLAARG
                     LPLRFVDATGSTAGLLQALIAAAPQCDVLLIHAGASDLCRLFGRSAGAAERDGSVKCR
                     PLLLADDRPTSVTHAYAAMKLLTQRAGLVVHDLLLGAARHSPRSERIAMQIATCADDF
                     LGAVLRDWVQIDPACDASEAPSPELRRLVREQLRSAHGEDAFAARPPHLPTRAGATPA
                     WSQP"
     gene            1900..2613
                     /locus_tag="VNU48_05710"
     CDS             1900..2613
                     /locus_tag="VNU48_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HWI10807.1"
                     /translation="MYTAKGQLDLNTTLKQYSSLVRRLAHQMIAKLPANVEIDDLIQV
                     GMIGLADAMTRFDAAQGVQFETFATQRIRGAMLDELRGNDYLSRGTRKQQRTIESAVH
                     KLEQKLGRAPVESEIAREMGLALGEYQELLGKVRGTQLVYLEDMSGDEGDQDFLDRHI
                     GVEEGNPLAQLQDQRMREALVEAIKVLPEREQYVMSMYYEHDMNLKEIAAVLKVTESR
                     VCQMHSQSIARLRVKLRAH"
     gene            2677..3411
                     /locus_tag="VNU48_05715"
     CDS             2677..3411
                     /locus_tag="VNU48_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI10808.1"
                     /translation="MRIAWPLPAFLSWALGWALFTLLRAVDAPQALAITAAVALGAAL
                     SLLGSTPWRRIFVAAGFPLSLAASGLAASMPAWGWLLPLALLALVYPLNAWRDAPLFP
                     TPGGALDGLARLAPLAPNARVLDAGCGLGDGLRELRREYPEAALHGLEWSWPLRVLCA
                     WRCRFAQVRRADIWAADWSGCDLVYVFQRPESMGRAAEKAARELRPGAWLVSLEFDAP
                     GVAPTKVIECADGRRLWLYRVPFRHR"
     gene            complement(3516..4445)
                     /locus_tag="VNU48_05720"
     CDS             complement(3516..4445)
                     /locus_tag="VNU48_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI10809.1"
                     /translation="MRPFKAFLICVALLAVALAGLGAYAPLRVLNTLAAAGGGVSRQE
                     GVAYGPLERQRLDVYTPTTAAPPRGWPVVVFFYGGSWNTGERAEYRFVGSALAARGVL
                     TLVADYRLYPAVRYPEFLRDSALALAWGLTQAPRLGGDPARVFVMGHSAGGYNAAMLA
                     LDPRWLAGTGHAPAELAGWIGLAGPYDFFPTDNREAQPVFFHPDYPANAQPIDFAAAK
                     GRPVFLGAPLDDAVVNPNRSTRALAARQLASGSAVTSRLYPRASHATLIGAFAAPLRW
                     IAPVLDDVLAFIAATPAVQHGTAFASTIDTVGG"
     gene            complement(4487..>4936)
                     /locus_tag="VNU48_05725"
     CDS             complement(4487..>4936)
                     /locus_tag="VNU48_05725"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746508.1"
                     /note="catalyzes the formation of arginine from
                     (N-L-arginino)succinate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWI10810.1"
                     /translation="LMKGQPMTYNKDNQEDKEPLFDSVDTLKDTLRIFIELVAGIAVK
                     PEAMERAARRGYATATDLADYLVKKGLPFRDAHEVVAHAVRFAIERGMDLSELPLETL
                     QSFNAGIADDVYDVLSLRGSLNARSVLGGTAPAQVRAQIARHRARLA"
BASE COUNT          701 a         1770 c         1776 g          689 t
ORIGIN      
        1 cgcgacacgc tgccggtcgc gcccgaaccg ccggtgccgg cgcttgccga ccacgcgcac
       61 ccgggccacg ccgcgacgcg tcgcgatcag gaagacatga tgagcgagct gcgctcgatg
      121 aagggcctga tcgaggagcg cttcggcgcg ctggccttca tggagaagct gaaccgccag
      181 ccggcccagg cgcgcctgac gcaaaagctc ctcgactgcg gcttctcgcc cgcgctgatc
      241 cgcaagatcg gcgacagcct gccgatggac gtgagcgacg aatcggcctg ggccgcgggc
      301 gtgctcgagc gcaacctgct gaccgccgag gccgaggccc cgctcgagga ccagggcggc
      361 gtgtacgcgc tggtcggcgc caccggcgtc ggcaagacca cctcgaccgc gaagctcgcg
      421 gccgcgttcg ccgccaagta cggcgccgcc aacctcggcc tgatcacgct cgacgcctac
      481 cgcatcggtg cccacgagca gctgcgcgcc tacggccgca tcctcggcgt gccggtgcac
      541 accgcgcacg accgcgcctc gctcgaggac ctgctggaac tgctcggcgg caagaagatg
      601 gtgctgatcg acactgccgg tatggcgcag cgcgacaccc gcacccgcga actgctggac
      661 atgctcagcc accgctcgat ccgcaagctg ctcgtcatca acgccgcctc gcagggcgag
      721 accatcgagg acgtgctgat cgcctaccag gccgcggcct gcgccggcat cgtgctgtcg
      781 aagatggacg aggccgtcaa gctcggcccg gcgctggacg tgctgatccg catgaagctc
      841 aaggtcgtcg gcgtggccaa cggccagcgc gtgccggaag actggcaccg cctgtcggcc
      901 aacgcgctgg tgcaccgcgc actgcgcggc ggcggcagca gcgcctaccg gctcgacgcg
      961 agcgacgtga gcctgatctt caccgcgccg atggcgtcga cccgcgcgcc ggcccgcggc
     1021 gcgctgcacg gctgatccga tgaaccgctt cttcgatgac gacctgacca tgtcccgacc
     1081 gatgccgctc gaccaggccg atggcctgcg ccgcctgttc gcccactcgc gcgtttgtgt
     1141 gctgccggtg gtctcgaacc ccaacgtcgc gttcggcggc gtgatgctcg aacggctgtg
     1201 caccgcgttc gccgagcacg gccgccacac gctggtggtc gatgccgccg agcgcgcccc
     1261 cttgcacagc gagatggcgc tgctcgacct ggccgagtgc atcgagccgc tgtcggcgca
     1321 ggtctcgtac ctggcagcgc gcggcctgcc gctgcgcttc gtcgacgcga ccggctcgac
     1381 cgccgggctg ctgcaggcac tgatagcggc cgcgccgcaa tgcgacgtgc tgctgatcca
     1441 tgccggcgcc tccgacctgt gccggctgtt cgggcgcagc gccggcgccg ccgagcggga
     1501 tggcagtgtg aagtgcaggc cgctgctgct cgccgacgac cgccccacca gcgtgaccca
     1561 tgcctacgcc gcgatgaagc tgttgacgca gcgcgccggc ctggtcgtgc atgacctgct
     1621 gctcggcgcg gccaggcact caccgcgctc ggaacgcatc gcgatgcaga tcgcgacctg
     1681 cgccgacgat tttctcggcg ccgtgctgcg cgactgggtc cagatcgacc cggcctgcga
     1741 tgccagcgag gcgccgtcgc ccgagctgcg ccggctggtg cgcgagcagc tgcgctcggc
     1801 ccacggcgaa gacgcgttcg cggcccggcc gcctcacctc ccgacgcgcg ctggcgcgac
     1861 cccggcatgg agccaacctt gaattccccg gagcaatcca tgtacaccgc caaaggccag
     1921 ctggacctga acacgacgct gaagcagtac agctcgctgg tgcggcgtct cgcgcatcag
     1981 atgatcgcca agctgccggc caacgtcgag atcgacgacc tgatccaggt cggcatgatc
     2041 ggcctggccg acgcgatgac gcgcttcgac gcggcccagg gcgtgcagtt cgagaccttc
     2101 gcgacgcagc gcatccgcgg cgcgatgctc gacgagctgc gcggcaacga ctacctctcg
     2161 cgcggcacgc gcaagcagca gcgcacgatc gagtcggcgg tgcacaagct cgagcagaag
     2221 ctcggccgtg cgccggtcga gagcgagatc gcgcgcgaga tgggcctcgc gctcggcgaa
     2281 taccaggagc tgctcggcaa ggtgcgcggc acccagctcg tctacctcga agacatgtcg
     2341 ggcgacgagg gcgaccagga ctttctggac cgccacatcg gcgtcgaaga gggcaacccg
     2401 ctcgcgcaac tgcaggacca gcgcatgcgc gaggccctgg tcgaggcgat caaggtgctg
     2461 cccgagcgcg aacagtacgt gatgagcatg tactacgagc acgacatgaa cctgaaggag
     2521 atcgcggcgg tgctgaaggt caccgagtcg cgcgtgtgcc agatgcacag ccagtcgatc
     2581 gcgcggctgc gcgtgaagct gcgcgcccac tgagccgccg ggtccgcgca tgctgatgat
     2641 gcgcaggcgt cgaagcgaag cgccgccgcg gccttgatgc gcatcgcctg gccgctgccg
     2701 gctttcctgt cgtgggcgct cggctgggcg ctgttcacgt tgctgcgcgc ggtcgacgcg
     2761 ccgcaggcgc tggcgatcac cgcggcggtc gcgttgggcg cggcgctgag cctgctgggc
     2821 agcacgccgt ggcggcgcat cttcgtcgcg gccggctttc cgctgtcgct cgcggcctcg
     2881 ggcctggccg cgtcgatgcc ggcctggggc tggctgctgc cgctggcgct gctcgcgctg
     2941 gtctacccgc tcaacgcgtg gcgcgacgcg ccgctgttcc cgaccccggg cggcgcgctc
     3001 gacggcctgg cgcggctcgc gccgctggcg ccgaacgcgc gcgtgctcga tgccggctgc
     3061 ggcctcggcg acgggctgcg cgagctgcgg cgcgagtacc ccgaggccgc gctgcacggc
     3121 ctcgagtgga gctggccgct gcgcgtgctg tgtgcctggc gctgtcgctt cgcgcaggtg
     3181 cggcgcgccg acatctgggc cgcggactgg tccggctgcg acctggtcta cgtgttccag
     3241 cgccccgaga gcatgggccg cgcggccgag aaggcggcgc gcgaactgcg tccgggcgcg
     3301 tggctcgtca gcctggaatt cgacgcaccg ggcgtcgcgc cgaccaaggt gatcgagtgc
     3361 gcggacgggc gccgcctgtg gctgtaccgc gtgccgttcc ggcatcgctg agttcgcgcg
     3421 cgaacggcgc cgccgctgca gcagtggttc gagctgccga tgtttgccca cgacgctcga
     3481 atcgctgcgc gcggccgaac ttcagaggta gtcgatcagc cgccgaccgt gtcgatggtc
     3541 gacgcgaacg cggtgccgtg ctgaaccgcc ggcgtggcgg cgatgaacgc cagcacgtcg
     3601 tcgagcaccg gcgcgatcca gcgcagcggc gcggcgaacg cgccgatcag cgtggcgtgg
     3661 ctggcgcgcg ggtacagcct cgaggtcacc gcgctgccgg acgccagctg acgagcggcc
     3721 agcgcgcgcg tgctgcggtt cgggttgacg accgcatcgt cgagcggcgc gccgaggaac
     3781 accgggcgcc ctttcgcggc cgcgaagtcg atcggctgcg cgttcgccgg atagtccggg
     3841 tggaagaaga ccggctgtgc ctcgcggttg tcggtcggaa agaagtcgta ggggccggcc
     3901 aggccgatcc agccggcgag ctcggccggc gcgtggccgg tgccggcgag ccagcgcggg
     3961 tcgagcgcga gcatcgccgc gttgtagccg ccggcgctgt ggcccatcac gaacacgcgc
     4021 gccgggtcgc cgcccaggcg cggcgcctgg gtcagacccc aggccagcgc gagcgcgctg
     4081 tcgcgcagga actcggggta acgcaccgcc gggtagagcc ggtagtcggc caccagcgtc
     4141 agcacgccgc gcgccgccag cgcactgccg acgaagcggt actcggcgcg ttcgccggtg
     4201 ttccacgagc cgccgtagaa gaacaccacc accggccagc cgcgcggcgg cgccgctgtg
     4261 gtcggcgtgt agacatcgag ccgctggcgt tcgaggggcc cgtaggccac gccttcctgc
     4321 cgcgagacgc cgccgccggc cgcggccagc gtgttcagca cgcgcagcgg cgcgtaggcg
     4381 cccaggccgg ccagggccac ggcgagcagg gcgacgcaga tcaggaaggc tttgaacggg
     4441 cgcaatcggc ggtccgggca atcactgagc cactgcgcgc ggtgcgtcag gccagccgcg
     4501 cccggtgccg cgcgatctgc gcacgcacct gcgccggtgc ggtgccgccg agcacactgc
     4561 gcgcgttcag cgagccgcgc aggctcagca cgtcgtagac atcgtcggcg atgccggcgt
     4621 tgaaggactg cagcgtttcc agcggcagct cggacaggtc catgccgcgt tcgatcgcga
     4681 acctgaccgc gtgcgcgacg acctcgtgcg cgtcgcggaa cggcaggccc ttcttgacga
     4741 ggtagtcggc caggtcggtc gcggtcgcgt agccgcggcg cgcggcacgc tccatcgcct
     4801 cgggcttgac ggcgatgccg gcgaccagct cgatgaagat gcgcagcgtg tccttgagcg
     4861 tgtcgacgct gtcgaacagc ggctccttgt cttcctggtt gtccttgttg taggtcatcg
     4921 gctggccctt catcag
//
LOCUS       DATMJB010000257         3798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_248512, whole genome shotgun sequence.
ACCESSION   DATMJB010000257 DATMJB010000000
VERSION     DATMJB010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248512"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /gene="shc"
                     /locus_tag="VNU48_05730"
     CDS             complement(<1..1396)
                     /gene="shc"
                     /locus_tag="VNU48_05730"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HWI10811.1"
                     /translation="MDIGTMDVETRQSGPVAAAELEASIGAATRALLEHRQPDGHWVF
                     ELEADCTIPAEYVLLRHYLGEPVDSALESKIANYLRRIQGAHGGWPLVHEGDFEMSAS
                     VKAYFALKMIGDSVDAPHMARAREAIRSRGGAVNSNVFTRFMLAMFGVLTWRAVPVLP
                     VEIMLLPMWSPFHLNKISYWARTTIVPLMVLAALKPRAKNVSGVGIDELFLQPPHSVG
                     MTAKAPHQSWGWFVLFRGLDSILRLIEPLFPRKLRARAIDSAVAFVEERLNGEDGLGA
                     IYPPMANTVMMYEVLGKGPDYPPRAITRRGIDRLLVIGEHEAYCQPCVSPVWDTSLTC
                     HALLEAGSEQALPPAKQGLDWLLPKQVLDIKGDWAVKRPDVRPGGWAFQYNNAYYPDL
                     DDTAVVVMAMDRAQRASQSHEYDEAIARGREWIEGMQSDDGGWAAFDVNNLEYYLNNI
                     PFSDHGALLDPPTED"
     gene            complement(1445..2698)
                     /gene="hpnE"
                     /locus_tag="VNU48_05735"
     CDS             complement(1445..2698)
                     /gene="hpnE"
                     /locus_tag="VNU48_05735"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HWI10812.1"
                     /translation="MQKTVHIVGAGISGLSAAVRLANANYNVHVHEATQTAGGRCRSY
                     FDAATNLTIDNGNHLLLSGNRHAIAYAKSIGTEAGLVGPKRAQFPFVDLTTGQRWQLD
                     LGDGRLPLWVFDEARRVPDTTLRDYLALAPLIWASTNKRVGDAIPCDGTLYQRLVQPL
                     LLAALNVDPPEGSAGLAGAIVRETLLAGGQACRPLIARDGLSAVLVEPAIKLLQDKGA
                     NIRFGHELREFAMSGSSVGELKFGDDTVTIGPDDAVVMAVPPRPAASLLPGLKTPSKF
                     RAIVNAHFRFDPPKGQPAILGVVGGLVEWLFAFPQRLSITISNGDRFVDMPREELAQA
                     IWRDICKAAGVQGELPPWQIVRERRATFEATPEQNALRPGPVTAWKNLFLAGDWTDTG
                     LPATIEGSVRSGDRAADLVLAMRNA"
     gene            complement(2698..3537)
                     /gene="hpnD"
                     /locus_tag="VNU48_05740"
     CDS             complement(2698..3537)
                     /gene="hpnD"
                     /locus_tag="VNU48_05740"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015878246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HWI10813.1"
                     /translation="MTVQTATPETNYGTSASGSSFYAAMRILPREQREAMFQIYSFCR
                     EVDDIADSDGPRPARLAALQQWRDDIDALYQGHPPPRLQDYVASVRRFGLKREDFLAI
                     VDGMEMDVPQDIRAPDLATLDLYCDRVASAVGRLSVRVFGLPEEDGILLAHHLGRALQ
                     LTNILRDIDEDATLGRLYLPREGLLHAGITSDDPAKVIPNPALPKVCVPLVTRARMHF
                     GKADEIMARNSRRVVRAPRIMSKYYHAILELLVARGFALPRAPVRLNKMARIAILLRH
                     AFI"
     gene            complement(3534..>3798)
                     /locus_tag="VNU48_05745"
     CDS             complement(3534..>3798)
                     /locus_tag="VNU48_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10814.1"
                     /translation="AARTEIVLNEGKSLAAEVRDFRLGLEVAVIQAFADRIVGLLKVR
                     DPLSERVHLSPLELFGQSVGGIASEITRRAVGRRPLSHPAAGA"
BASE COUNT          677 a         1272 c         1208 g          641 t
ORIGIN      
        1 cgtcctcggt cggcggatcg agcagcgcgc cgtggtccga gaacggaatg ttattgaggt
       61 agtattccag attgttgacg tcgaaagcgg cccagccgcc atcgtcgctc tgcatgccct
      121 cgatccactc gcggccgcga gcgatcgcct cgtcatactc gtggctttgc gacgcgcgct
      181 gcgcgcgatc catcgccatg accacgacag cggtgtcgtc gagatcggga taataggcgt
      241 tgttgtactg aaacgcccag ccgccgggac gaacgtccgg ccgcttcacg gcccagtcgc
      301 ccttgatatc cagcacctgc ttcggcaaca gccagtcgag gccctgcttg gcaggaggca
      361 aggcctgctc gctgccggct tccagcagcg catggcaggt cagcgacgtg tcccacaccg
      421 gcgagacgca aggctggcag taggcctcgt gctccccgat caccagaagc cggtcgatgc
      481 ccctacgcgt gatggcgcgc ggcggataat ccggcccctt gccgagcact tcgtacatca
      541 tcacggtgtt ggccatcggc ggatagatcg cgccgagacc gtcttcaccg ttcaggcgct
      601 cctcgacgaa cgcgactgcg ctgtcgatgg cgcgcgcgcg cagcttccgc gggaacagcg
      661 gttcgatcaa ccgcaagatg ctgtcgagcc cgcggaacag cacaaaccag ccccagcttt
      721 gatgcggcgc cttggctgtc atcccgaccg aatgcggcgg ctgcaggaac aattcgtcga
      781 tgccgacgcc gctgacattc ttcgcacgcg gcttgagcgc cgccagcacc atcagcggca
      841 cgatcgtggt gcgcgcccag taggaaatct tgttgagatg aaacggcgac cacatcggca
      901 gcagcatgat ctcgaccggc aacaccggaa cggcgcgcca ggtcagcacc ccgaacatcg
      961 ccagcatgaa gcgggtgaat acgttgctgt tgacggcgcc gccgcgcgag cggatcgctt
     1021 cgcgcgcccg tgccatgtgc ggcgcgtcca cggaatcgcc gatcatcttc agcgcgaaat
     1081 aggccttcac gctggcgctc atttcgaagt cgccctcatg caccagcggc cagccaccat
     1141 gagcgccctg aatccggcga agataattgg cgatcttgga ttccagcgcg gaatcgaccg
     1201 gctcgccgag ataatggcgc agcagcacat actcggctgg gatggtgcaa tcggcttcga
     1261 gctcgaacac ccagtgtccg tcgggctggc ggtgctcaag cagcgcgcgc gtcgccgccc
     1321 cgatactggc ttccagttcg gccgcggcta ccggcccgct ttgtctcgtt tccacatcca
     1381 tcgtgccaat atccattcct gttcgctccg catcgtctcg ccggactatg cctgacattg
     1441 acgctcaggc gttccgcatg gcgaggacca gatcggcggc gcggtcgccc gagcgcactg
     1501 agccttcgat ggtcgccggc aacccggtat cagtccagtc gccggcgaga aacaggtttt
     1561 tccaggccgt gaccggcccc ggacgcagcg cattctgttc cggcgtggcc tcgaaggtgg
     1621 cgcggcgctc gcgcacgatc tgccagggcg gcaattcacc ctgtacgccc gccgccttgc
     1681 agatatcccg ccagatcgcc tgcgcgagct cctcgcgcgg catgtcgacg aagcggtcgc
     1741 cattgctgat cgtgatcgac agccgctgcg ggaacgcgaa cagccattcc accagaccgc
     1801 cgacgacgcc gaggatcgcc ggctggccct tgggcggatc gaagcggaaa tgcgcattga
     1861 cgatcgcgcg gaatttcgac ggcgttttga ggcccggcaa caatgacgcg gcggggcgcg
     1921 gcggaaccgc catcaccacc gcgtcatcag gcccgatggt gacggtgtcg tcgccgaact
     1981 tcaattcgcc gacgctgctc cccgacatcg cgaattcgcg cagctcatgc ccgaaccgga
     2041 tattggcgcc cttgtcctgc aacaacttga tcgccggttc gaccagaacg gcgctgaggc
     2101 cgtcgcgcgc gatcaacggc cggcaggcct gcccgcccgc gagcagggtc tcgcgcacga
     2161 tcgcgccggc taatcctgca gagccctccg gcggatcgac attgagggcc gccagcagca
     2221 gcggctgcac cagccgctga tagagcgtgc cgtcgcaagg gatggcatcg ccgacccgct
     2281 tgtttgtcga cgcccaaatc agcggcgcca gcgcaagata gtcgcgcagc gtcgtatcgg
     2341 gaacgcggcg cgcttcgtcg aacacccata gcggcaatcg gccgtcgccg agatcgagct
     2401 gccagcgctg gccggtcgtg aggtcgacga acggaaactg cgcgcgcttc ggcccgacca
     2461 ggcctgcctc ggtgccgatc gatttggcat aggcgatcgc atggcggttg cccgacagca
     2521 gcagatggtt gccattgtcg atggtcaggt tggtcgcggc gtcgaaatag gaccggcaac
     2581 ggccgcccgc ggtctgggta gcctcgtgga catgcacgtt gtaattggca ttggccagcc
     2641 ggacggctgc cgagaggcct gaaattccag caccgacgat gtgaacggtt ttttgcatca
     2701 gatgaaggcg tggcgaagca ggatggcgat gcgcgccatt ttattgaggc gaaccggcgc
     2761 acgcggcaag gcgaagcccc gcgccaccag caattcgagg atcgcatgat aatatttcga
     2821 catgatccgg ggcgctcgca ccacgcgccg cgagttccgg gccatgatct cgtcggcctt
     2881 gccgaaatgc atccgcgccc gcgtcaccag cggcacgcac actttcggca gcgccggatt
     2941 gggaatgacc ttggccggat cgtcgctggt gatgcctgca tgcaacagcc cctcgcgcgg
     3001 cagatacagc cggccgagcg tggcgtcctc gtcgatgtcg cgcaggatgt tcgtcagttg
     3061 cagcgcgcgg ccgagatgat gcgcgagcaa gatgccgtct tcttccggca gcccgaatac
     3121 ccgcaccgac agccggccga cggcgctggc gacgcgatcg caatatagat cgagcgtggc
     3181 gagatccggc gcgcggatat cctgcggcac gtccatctcc ataccgtcga cgatggccag
     3241 gaaatcctcg cgcttgagac cgaatctccg caccgaggcg acgtagtcct gcaaccgcgg
     3301 cggcggatga ccctgataca gcgcgtcgat atcgtcgcgc cattgctgaa gcgctgccag
     3361 ccgcgcgggt ctcggaccgt cggaatccgc gatgtcgtcg acctcccggc agaaactata
     3421 gatctggaac atcgcctcgc gctgttcgcg cggcagaatc cgcatcgccg cgtagaacga
     3481 gctgcctgac gcggaggtcc cgtaatttgt ctccggggtt gcggtctgaa ccgtcatgcg
     3541 ccggccgccg gatgtgacag gggccgccgt ccgaccgcgc ggcgggtgat ctcggaggcg
     3601 ataccgccga cgctctgccc gaacagctcg agcgggctga gatgcacccg ttcgctcagg
     3661 ggatcgcgca ccttgagaag gccgacgatc ctgtcggcga acgcctggat cacggcgacc
     3721 tcgagcccga gccggaaatc cctgacttcc gcggcgagcg acttgccttc attgagcacg
     3781 atttcggtcc tggccgcg
//
LOCUS       DATMJB010000258         2257 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_852460, whole genome shotgun sequence.
ACCESSION   DATMJB010000258 DATMJB010000000
VERSION     DATMJB010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852460"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VNU48_05750"
     CDS             <1..250
                     /locus_tag="VNU48_05750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657638.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWI10815.1"
                     /translation="LKQNQYMPLPISLMSASLFAVNKGFMDDIDVKKVLAFEHGLHTH
                     LKSSHGPLLKKIEDSKALDKDAEAELTSAITAFKKSFA"
     gene            273..1154
                     /gene="atpG"
                     /locus_tag="VNU48_05755"
     CDS             273..1154
                     /gene="atpG"
                     /locus_tag="VNU48_05755"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HWI10816.1"
                     /translation="MAAGKEIRGKIKSFENTKKITKAMEMVSVAKMRKAQDRMRAARP
                     YAEKIRSLASNLATATPEYKHPLMVKNEAAKTVGFILVTTDKGLCGGLNTNVLRAVTA
                     KLREVDASGQTSESVAIGNKGLGFLNRIGAKVVSHATHLGDKPTLARLIGPVKVLLDA
                     YAEGKLSAVYLCYTRFINTIKQEPVIHQLLPLTAEGMRGEAAPDGPRHGWDYLYEPDA
                     PAVIDELLVRYTEALVFQALAENMASEHSARMVAMKAATDNAGNLIGELKLIYNRTRQ
                     AAITKELSEIVAGAAAV"
     gene            1212..>2257
                     /gene="atpD"
                     /locus_tag="VNU48_05760"
     CDS             1212..>2257
                     /gene="atpD"
                     /locus_tag="VNU48_05760"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWI10817.1"
                     /translation="MADGKIVQCIGAVVDVEFPRNAMPKVYDALIMEGGALTLEVQQQ
                     LGDGVVRTIALGSSDGLRRGLTVSDTGKPISVPVGRATLGRIMDVLGRPIDERGPVGQ
                     ELTMPIHRKPPSYDELSPSQDLLETGIKVIDLVCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKEHSGLSVFAGVGERTREGNDFYHEMSDSKVVDLENLGNSKVAMVYGQMNEP
                     PGNRLRVALTGLTIAESFRDEGKDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPT
                     LAEEMGRLQERITSTKVGSITSIQAVYVPADDLTDPSPATTFAHLDSTVVLSRDSASL
                     GIYPAVDPLDSTSR"
BASE COUNT          445 a          745 c          738 g          329 t
ORIGIN      
        1 gctgaagcag aaccagtaca tgccgctgcc gatctcgctg atgagcgcgt cgctgttcgc
       61 ggtcaacaag ggcttcatgg acgacatcga cgtcaagaag gtgctcgcgt tcgaacacgg
      121 cctgcacacg cacctgaagt cgagccacgg cccgctgctg aagaagatcg aagacagcaa
      181 ggcgctcgac aaggatgccg aagccgagct gacgagcgcg atcaccgcgt tcaagaagtc
      241 cttcgcctga cccattagga gtaggagccg tcatggcagc aggcaaagag atacgcggca
      301 agatcaagag cttcgagaac acgaagaaga tcaccaaggc catggagatg gtctcggtgg
      361 cgaagatgcg caaggcgcag gaccgcatgc gcgcggcgcg cccgtacgcc gagaagattc
      421 gcagcctggc gtcgaacctg gccaccgcca cgcccgaata caagcacccg ttgatggtca
      481 agaacgaggc cgccaagacg gtcggcttca tcctcgtcac gaccgacaag ggcttgtgcg
      541 gcgggctgaa caccaacgtg ctgcgcgccg tcacggccaa gctcagggaa gtcgacgctt
      601 cggggcagac cagcgagtcg gtggcgatcg gcaacaaggg gttgggcttc ctgaaccgca
      661 tcggtgccaa ggtcgtctcg cacgcgacgc acctgggcga caagcccaca ctggcccggc
      721 tgatcggccc ggtcaaggtg ctgctcgacg cgtacgccga gggcaagctg tcggcggtct
      781 acctgtgcta cacgcgcttc atcaacacga tcaagcagga gccggtgatc caccagctgc
      841 tgccgctgac ggccgaaggc atgcggggcg aggccgcgcc cgatggcccc cggcacggct
      901 gggactatct gtacgagccc gacgcgccgg ccgtgatcga cgagctgctg gtgcgctaca
      961 ccgaggcgct ggtgttccag gcgctggccg agaacatggc gagcgaacac tcggcgcgca
     1021 tggtcgcgat gaaggcggcg accgacaacg cgggcaacct gatcggcgag ctcaagctga
     1081 tctacaaccg cacgcgccag gcggcgatca ccaaagaact gtccgagatc gtggccgggg
     1141 ctgccgccgt ctagcgcggt ggcgtcacac ccacggtccg caacacaaag atttgagtca
     1201 aaggaaaaat catggctgac ggcaaaattg tccagtgcat cggcgcggtg gtggacgtcg
     1261 agttcccccg caacgcgatg cccaaggtgt acgacgcgct gatcatggaa ggcggcgcgc
     1321 tcacgctcga ggtgcagcag cagctcggcg acggtgtggt gcgcacgatc gcgctcggct
     1381 cgtccgacgg cctgcgccgc ggcctgaccg tctccgacac cggcaagccg atctcggtgc
     1441 cggtgggccg cgcgacgctc ggtcgcatca tggacgtgct cggccgcccg atcgacgagc
     1501 gcggcccggt cggccaggaa ctgacgatgc cgatccaccg caagccgccg tcgtacgacg
     1561 agctcagccc gtcgcaggac ctgctcgaga ccggcatcaa ggtgatcgac ctcgtgtgcc
     1621 cgttcgccaa gggcggcaag gtcggcctgt tcggcggcgc cggcgtcggc aagaccgtga
     1681 acatgatgga gctgatcaac aacatcgcga aggagcactc gggcctgtcg gtgttcgccg
     1741 gcgtcggcga gcgcacccgc gagggcaacg acttctatca cgagatgtcc gactccaagg
     1801 tcgtcgacct cgagaacctc ggcaactcga aggtcgcgat ggtctacggc cagatgaacg
     1861 agccgccggg caaccgcctg cgcgtcgcgc tgaccggcct gacgatcgcc gagtcgttcc
     1921 gcgacgaagg caaggacgtg ctgttcttcg tcgacaacat ctaccgctac acgctggccg
     1981 gtaccgaagt gtcggcactg ctgggccgca tgccctccgc cgtgggctac cagccgacgc
     2041 tggccgaaga aatgggccgc ctgcaggaac gcatcacgtc gaccaaggtc ggctcgatca
     2101 cgtcgatcca ggccgtctac gtgccggcgg atgacttgac cgacccgtcg cctgccacca
     2161 ccttcgccca cttggattcc accgtggtgc tgtcgcgtga cagcgcctcg ttgggcatct
     2221 accccgccgt ggacccgctc gactcgacct cgcgcca
//
LOCUS       DATMJB010000259         9061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_402685, whole genome shotgun sequence.
ACCESSION   DATMJB010000259 DATMJB010000000
VERSION     DATMJB010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9061
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402685"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5879)
                     /locus_tag="VNU48_05765"
     CDS             complement(<1..5879)
                     /locus_tag="VNU48_05765"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10818.1"
                     /translation="MTNIIGGYDTGLFDSSLFLLNRDDRTSQGAAGHGEEMYVNVANG
                     NLVVLHKDAFLPSQGDDYLLVRTYNSRGIWGSTDGKGWSNNSTVLELSQITNNVITLV
                     NADTSRARFTYDAASDTYISVDGSGAYEVITFDKTSKRYSLTTSNQTVLSFDNTGNLI
                     SSKDTSGNLIEYTYAQGKLQSLKDDQGHVISYVYSNGDLVRVQDETGAVLVQYEYSQG
                     AITAVIDRSGHRTTYAYYTDGTLKSITLPAGSGDPTRQLNFAYDADPTDSTGKTRLLR
                     WLTDAEGNRTYFEYQFNVDNFSKYNGGTTVVVSALGINRRESNDTQYVEWRLANGFYA
                     TWNATTYANDASYRAQADAITSRHTVRYGYDKNGAITSVVDQAGYQTTYQYDAKENLT
                     TVVDADGYAITRSDNAYWRDLRRDYGYVDLAGQGKQVVGLSTAEIAALLERYTTHFEY
                     DARGNLTKKTDNADNVTSFTYTSFNKVATQIAAMGNALVTSNDSVTQDKRAELGYARD
                     LAALSTADKAALLALYTTTYTYDAKQNLTELKSPGGDLTRYEYDSYGNLTKRTVYLDA
                     ADLVSAAKQQVTQYFYDAYGQNIKTIDAEGYTSYSSYDHFGNLLTHTDARGGVTTYTY
                     DADNQVLTVTDPEGNTTAYTYDAVGNRTAVRDANGHSVVYVYDRNNMLITTVDPKDGD
                     SSGNRVTAYSYDVVGNRIKAVDAEGRISTYVYREDNRLLEVRTPTVANAAGTGSTSYS
                     SRYEYDGVGHQIAVTDNNGNRSQYVFNESGLLKRATDAAGNVTEHRYDANLNQIQIVI
                     GAQLAVEKRRVLRFSFDEEDQRIAETDALGGSTRYTRDAVGNVVATTDANGHGTEYRF
                     DRNNRLVAEVRPGVIDNATGQTVRHTATHQYDGNGNQIASTDENGHTTRLSFDKDDRL
                     ILVEDANGIKTAYSYDSRSNRTQVAIGVQAHLDAAGKVVIDSSGDAQVQTFAYDEFNQ
                     IVSRTDGVGTALASSDSNLYRAMRQQMGYAAALASLSAADKQALLAAYTERYAYDKVG
                     NTVTVTDHLGRVTRMAYDGLNRETSRTDALGQVQSRRYDGNGNRVAFVDALGHEQRSA
                     YDAVNRLVSQTDALGVVTLNEYDVVGNLLASTQASGSADARSTHYEYDLDNRLTRQID
                     AEGNSQAYEYDAVGNRLKRIDARGNATRYVYDARDRLVKTIDPLSFETKIEYDGVGNR
                     IALIDARGGLTRFDYDPGNRLVATTDAEGRITRFEYDLRGNRITQRSAAGTADEQLTH
                     FEYDAQNQLRAVVDAEGNRSSSDFDREYNRIASTDGNGHTSHYAFDALNRTISVTDAL
                     GAETRYAYDAVGNQLSQTDALGRVTSFVYDADNRVTTQTTADGVVTRYAYDAVDNRIA
                     LTRAFGLPEAQTTVYAYDKNNRLVAQTDALGHTTRYEFDPNGNAVASIDALNQRSTYV
                     YDADNRVVQIVDPLGQTTGYRYDAAGNRTQVIDALGRTSTSYYNLDNELVLAVNAEGE
                     ATRYSYDHNGNRVSETRFATRLTAALDPAQPPTVASAAADQTTRYAYDHLNRLVRRLD
                     AEGNLVDTAYDAAGNLVSTTAYASAVSLGSASLASLVPVTSAKDRLTRFGYDADNRQI
                     ERIDAEGYLTRWSYDAVGNRSAQTRYLDKTDLVDVAKQQRTEYRHDALNRLTGELSPI
                     GVETRYTYDALGQRTAQTDAYGTPAERSTAYHYDAAGRLVEQVDAAGTVTRYVLDAAG
                     QITQQTVAFGQPEARATSYRYDAAGRRIELIAADATVQRSVYDAVGNLTLRIDAAGTP
                     AERSTAFAYDRANRLLSQTVGVGQPQALVTSYTRDAFGNRTRQTIGAGSDDARVTQYA
                     YDHLDRLIAQTDGNQIVTLNDYDAFGNRVLTRITGSTLTAGGTSVQRTEAARFEYDGR
                     NLLIGERNGVDTLIERQYDGAGNLRFQTAAAGTASASTTEFRYDLANRLTTKVVDPAG
                     LA"
     gene            6136..7347
                     /locus_tag="VNU48_05770"
     CDS             6136..7347
                     /locus_tag="VNU48_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI10819.1"
                     /translation="MQSDLLTRAERLQGYAAQDPTNLALLRDLAATWHAAGEHQRALD
                     ALDRAEAAAGGPSALAPLRGPVLLALGRWQEAAAIFENGLQTQPDSAALAFNLGYAVW
                     ASEQDPARAVELFRQAARIEPRDARLRYYLALALESVADLDATRQSLDEALAIDPRHI
                     DALAMLGRLALDAGELERATDLAARCIAARPDHPAGWQLKGQAALFRMDAADAVKSLR
                     QALDLAPDDVDTRVSLAQAALMQGRPRHAKDLLQQAVAENASHDTALCMLGWACIADN
                     EAAGAAAAFERANTAAPDNPEAMAGIALLRLADGRRDEALQWTERALALEPAHAMAGL
                     LRARLAELDDRPAEARTVLASVLDGVSSGPLGASTLGQKLAAAGASPTVGRMKRRFAR
                     ALPAVPKNRSR"
     gene            7389..7970
                     /locus_tag="VNU48_05775"
     CDS             7389..7970
                     /locus_tag="VNU48_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI10820.1"
                     /translation="MTLSQATELDSEELLHLAVAASDRDQTDQAIGLLKRAIDVDPSN
                     ARAHYLLGAEHAQIGLFDRAAQEMRRALELDGELDAARFQLGLLLLTSRQVEPAESAW
                     KHLDRLGTGHFYVLFKTGLLCLANDEFSESLRCLRDGITANQENAPLNADMQRIAQQV
                     EAILAQPGAAASADAEDSSAAHLLINAYTGRRH"
     gene            8000..8374
                     /locus_tag="VNU48_05780"
     CDS             8000..8374
                     /locus_tag="VNU48_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10821.1"
                     /translation="MDPVSKLTKTLAAFRRITGSPAPAGGKVKSQAGQAASGRTPAST
                     VGELHAEIARRLGMVSPLDPNRRDAAVKVFLEQVLLHEFGPQLLTSQRFSDMVRSVQQ
                     TMDADPAVKAQLDDLIGEVSAR"
     gene            complement(8515..>9061)
                     /locus_tag="VNU48_05785"
     CDS             complement(8515..>9061)
                     /locus_tag="VNU48_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWI10822.1"
                     /translation="WGEVDWERAQWLIPADRMKAGKPHTVYLAEQALDILTTLRTCFP
                     SSSFVHPSRYDSSEPISQATLNRTIAAAVDRINEDRTPDQGLFMPVSVHDLRRTFSSR
                     LNDALFPEALIEACLAHQKKDHIAAAYNHARLSGPRRVLMQVWADMLDCWMRGESARE
                     AIVRGKAKIDAAAHDAEGMNL"
BASE COUNT         1217 a         2797 c         3268 g         1779 t
ORIGIN      
        1 gctaggcccg cggggtcgac gaccttggtg gtcaggcggt tggcgaggtc gtagcgaaac
       61 tcggtggtgc tggccgacgc ggtgccggcc gcggcggtct ggaaacgcag gttgccggcg
      121 ccatcgtatt ggcgctcgat cagcgtgtcg accccgttgc gctcgccgat cagcaggttg
      181 cggccatcgt attcgaagcg tgctgcctcg gtgcgctgca ccgaggtgcc gcccgccgtc
      241 agcgtgctgc cggtgatgcg ggtcagcacg cgattgccga acgcgtcgta gtcgttcagc
      301 gtgacgatct ggttgccgtc ggtctgcgcg atcaggcggt cgaggtggtc gtaggcgtac
      361 tgggtgacgc gggcgtcgtc gctgccggcg ccgatggtct ggcgcgtgcg gttgccgaac
      421 gcgtcacggg tgtaggaagt gaccagcgcc tgcggctgcc ccacgcccac ggtctggctc
      481 aacaggcggt tcgcgcggtc gtaggcgaag gcggtgctgc gctcggccgg cgtgccggcg
      541 gcatcgatgc gcagcgtcag gttgcccacc gcgtcgtaga cgctgcgctg cacagtggcg
      601 tcggcggcga tcagttcgat gcggcgcccg gcggcgtcgt agcggtagct ggtggcgcgc
      661 gcctcgggct gaccgaaggc gacggtctgc tgcgtgatct ggcccgcggc gtcgagcacg
      721 tagcgcgtga cggtgccggc cgcgtcgact tgctccacca gccggccggc ggcgtcgtag
      781 tggtaggcgg tgctgcgttc ggccggcgtg ccgtaggcgt cggtctgcgc ggttcgttgg
      841 cccagcgcgt cgtaggtgta gcgggtctcg acaccgatcg ggctcagctc gccggtcagg
      901 cggttcagtg cgtcatggcg gtattcggtg cgctgttgtt tcgcgacatc gacgagatcc
      961 gttttgtcga ggtagcgggt ctgcgcgctg cggttgccga ccgcgtcgta gctccagcgg
     1021 gtcaggtagc cctcggcgtc gatgcgctcg atctgccggt tgtcggcgtc gtagccgaac
     1081 cgggtcaatc ggtctttcgc gctggtcacg ggcaccaggg acgccaggct ggcgctgccg
     1141 agcgagaccg cgctggcata ggcggtggtc gatacgaggt tgccggccgc gtcataggcg
     1201 gtgtcgacca ggttgccttc cgcgtcgagg cggcggacca ggcggttcag gtggtcgtag
     1261 gcgtagcggg tggtctggtc ggcggcggcg ctggccaccg ttggtggctg cgccgggtcg
     1321 agcgcggcgg tcaagcgcgt ggcaaagcgc gtctcgctga cgcggttgcc gttgtggtcg
     1381 tagctgtagc gggtcgcctc gccctcggcg ttgaccgcca ggaccagctc gttgtcgagg
     1441 ttgtagtagc tggtgctcgt gcggcccagc gcatcgatca cctgggtgcg gttgcccgcg
     1501 gcgtcgtagc ggtagccggt ggtctggccg agcggatcga cgatctgcac gacgcggttg
     1561 tcggcgtcgt agacataggt gctgcgctgg ttcagcgcat cgatgctggc gacggcattg
     1621 ccgttcgggt cgaactcgta gcgagtggtg tggccgagcg cgtcggtctg ggcgacgagc
     1681 cggttgttct tgtcataggc gtagacggtg gtctgcgcct cgggcaggcc gaacgcgcgc
     1741 gtcagcgcga tgcggttgtc caccgcgtcg taggcgtagc gcgtgaccac gccgtcggcc
     1801 gtggtctggg tggtgacgcg gttgtcggcg tcgtagacga aactggtcac gcgcccgagc
     1861 gcgtcggtct ggctgagctg gttgcccacc gcgtcgtagg cgtagcgggt ctcggcgccg
     1921 agcgcgtcgg tgacgctgat cgtgcggttc agcgcgtcga aggcgtagtg gctggtgtgg
     1981 ccgttgccgt cggtgctggc gatgcggttg tattcgcggt cgaagtcgct gctgctgcgg
     2041 tttccttcgg cgtcgacgac ggcgcgcaac tggttctgcg cgtcgtactc gaagtgggtg
     2101 agctgctcgt cggccgtgcc cgccgcgctg cgctgggtga tacggttgcc gcgcaggtcg
     2161 tactcgaagc gggtgatgcg cccttcggcg tcggtcgtgg cgacgaggcg attgccgggg
     2221 tcgtagtcga agcgggtcag gccgccgcgc gcatcgatca gtgcgatgcg gttgcccacg
     2281 ccgtcgtact cgatcttggt ctcgaagctg agcgggtcga tggtcttgac gagccggtcg
     2341 cgggcgtcgt agacgtagcg ggtcgcgttg ccgcgcgcgt cgatgcgctt gaggcggtta
     2401 ccgaccgcgt cgtattcgta ggcctggctg ttgccttcgg cgtcgatctg gcgggtcagc
     2461 cggttgtcga ggtcgtattc gtagtgcgtg ctgcgggcgt cggcgctgcc ggacgcctgc
     2521 gtgctcgcca gcaggttgcc caccacgtcg tattcgttga gcgtgaccac gcccagcgcg
     2581 tcggtctggc tcaccaggcg gttcactgcg tcgtaggcgg agcgctgctc gtggcccagc
     2641 gcgtcgacaa aggcgacgcg gttgccgttg ccgtcgtagc ggcggctttg cacctggccg
     2701 agcgcgtcgg tgcgcgatgt ctcgcggttc aggccgtcgt aggccatgcg cgtgacgcgg
     2761 ccgaggtggt cggtgaccgt gacggtgttg ccgaccttgt cgtacgcgta gcgctcggtg
     2821 taggcggcca gcagcgcctg cttgtcggcc gcggacaggc tggccagggc ggcggcgtag
     2881 cccatctgct ggcgcatcgc gcggtacagg ttgctgtcgg agctggcgag tgcggtaccg
     2941 acgccatcgg tgcgcgacac gatctggttg aactcgtcgt aggcgaaggt ctgcacctgc
     3001 gcgtcgccgc tgctgtcgat cacgactttg ccggcggcat cgagatgggc ttgcacgccg
     3061 atcgcgacct gggtgcggtt gctgcgcgag tcgtagctgt aggcggtctt gatgccgttg
     3121 gcgtcttcga ccaggatcag ccggtcgtcc ttgtcgaagc tcaggcgcgt ggtgtggccg
     3181 ttctcgtcgg tgctcgcgat ctggttgccg ttgccgtcgt actggtgcgt cgccgtgtgg
     3241 cgcacggtct ggccggtggc gttgtcgatg acgccgggac ggacttccgc gacgagtcga
     3301 ttgttgcggt cgaagcggta ctcggtgccg tggccgttgg cgtcggtggt ggccaccacg
     3361 ttgccgaccg cgtcgcgggt gtagcgcgtg ctgccgccga gcgcgtcggt ctcggcgatg
     3421 cgctggtcct cctcgtcgaa cgagaagcgc agcacgcggc gcttctcgac cgccagctgc
     3481 gcaccgatca cgatctggat ctggttcaag ttcgcgtcgt agcggtgctc ggtgacgttg
     3541 ccggccgcat ccgtggcgcg cttgagcagg ccgctctcgt tgaagacgta ctgcgagcgg
     3601 ttgccgttgt tgtcggtcac cgcaatctgg tggccgacgc cgtcgtactc gtagcggctg
     3661 ctgtagctcg tgctgccggt gccggccgcg ttggcgaccg tgggggtgcg cacttcgagc
     3721 aggcggttgt cctcgcgata cacgtaggtg ctgatgcggc cctcggcgtc gacggccttg
     3781 atgcgattgc cgaccacgtc gtagctgtag gcggtgaccc ggttgccgct gctgtcgccg
     3841 tccttcggat cgacggtcgt gatcagcatg ttgttgcggt cgtagacgta cacgacgctg
     3901 tggccgttgg cgtcgcgcac cgcggtgcgg ttgccgaccg cgtcataggt gtacgcggtg
     3961 gtgttgccct cggggtcggt gaccgtcagg acctggttgt cggcgtcgta ggtgtaggtc
     4021 gtgacaccgc cgcgcgcatc ggtgtgcgtc agcagattgc cgaagtggtc gtagctgctg
     4081 tagctggtgt agccctcggc gtctatcgtc ttgatgttct gcccatacgc gtcgtagaaa
     4141 tactgcgtga cctgctgctt cgcggcgctg acgagatcgg ccgcatcgag atagacggtg
     4201 cgcttggtca ggttgccgta gctgtcgtac tcgtagcgcg tcaggtcgcc gcccgggctc
     4261 ttcagctcgg tcaggttctg cttggcgtcg taggtgtagg tcgtcgtgta cagggccagc
     4321 agcgccgcct tgtcggcagt gctcagtgcc gccaggtcgc gcgcatagcc gagctcggcg
     4381 cgcttgtcct gggtgaccga gtcgttcgac gtgacgagcg cgttgcccat cgccgcgatc
     4441 tgagtcgcga ccttgttgaa cgaggtgtag gtgaagctcg tgacgttgtc ggcgttgtcg
     4501 gtctttttcg tcaggttgcc gcgcgcgtcg tactcgaagt gcgtggtgta gcgctcgagc
     4561 agtgcggcga tctccgcggt actcagcccc accacctgct tgccctgccc ggccaagtcg
     4621 acatagccgt agtcgcggcg caggtcgcgc cagtaggcgt tgtcgctgcg tgtgatcgcg
     4681 tagccatcgg cgtcgacgac ggtggtcagg ttttccttgg cgtcgtactg gtaggtggtc
     4741 tggtagccgg cctggtcgac gacgctggtg atcgcgccgt tcttgtcgta gccgtatctc
     4801 acggtgtggc ggctggtgat cgcatcggcc tgcgcgcggt agctggcatc gttcgcgtag
     4861 gtggtcgcgt tccaggtcgc gtagaagccg ttcgcgaggc gccactcgac gtactgcgtg
     4921 tcgttggatt cgcgccggtt gatgccgagc gcgctgacga cgaccgtcgt gccgccgttg
     4981 tacttgctga agttgtcgac gttgaactgg tactcgaagt aggtccggtt gccttcggcg
     5041 tcggtcaacc agcgcagcaa gcgggtcttg ccggtgctgt cggtcgggtc tgcgtcgtag
     5101 gcgaaattga gctggcgcgt ggggtcgccg ctgccggccg gcagcgtgat gctcttcagc
     5161 gtgccgtcgg tgtagtacgc gtaggtcgtg cgatggccgg agcggtcgat gaccgccgtg
     5221 atcgcccctt gcgaatactc gtactggacc aggacggcgc cggtttcgtc ctgcacgcgg
     5281 acaagatcgc cattggagta cacgtagctg atgacgtggc cctggtcgtc cttcaggctc
     5341 tgcagcttgc cctgggcgta ggtgtattcg atcaggttgc cgctcgtgtc cttgctcgat
     5401 atcaggttac cggtgttgtc gaagctgaga acggtctggt tcgacgtggt caggctgtag
     5461 cgcttgctcg tcttgtcgaa ggtgatgacc tcgtaggcgc ccgagccgtc gacggagatg
     5521 taggtgtcgc tcgccgcgtc gtacgtgaac cgggcacggc tggtgtcggc gttgaccagc
     5581 gtgatcacgt tgttggtgat ctgcgagagc tcgagcacgg ttgagttgtt gctccagccc
     5641 ttgccgtcgg tgctgcccca gatgccgcgg ctgttgtagg tgcgcaccag cagatagtcg
     5701 tcgccctggg aaggcaggaa cgcatccttg tgcagcacga cgaggttgcc gttggccacg
     5761 ttgacgtaca tctcctcgcc atggcctgca gcgccctggc tggtgcggtc gtcgcggttg
     5821 agtaagaaca gcgagctgtc gaacaggccg gtgtcgtagc cgccgatgat gttggtcatt
     5881 ccctgagctc cagtgattcg ttcgcttggc cggcgcactc gcgccactac gtctgttccc
     5941 gcggcaccct caagcgcctt catgcaaggt atccggagga tttcgcgccg gtttagggct
     6001 gcgtcgaaat aaagtcgagg cgttgtgttt tggccttggc aagccgcaat tcgcggcccg
     6061 tcgatggccc gaaatcgagg cggttctgct acgctggcgc gaccgacagg atctctgcgc
     6121 tttttcattc cctccatgca aagcgacttg ctcacccggg ccgaacggct gcagggatac
     6181 gcggcccagg acccgaccaa cctggccctg ttgcgcgact tggcggccac atggcacgct
     6241 gccggcgagc accaacgcgc gctcgatgcg ctcgatcggg ccgaggcggc tgcgggcgga
     6301 ccttcggcgc ttgcgccgct gcgcgggccg gtcctgctgg cgcttgggcg atggcaagag
     6361 gccgcggcga tcttcgagaa cggactgcaa acgcaacccg actcggccgc gctcgcgttc
     6421 aatctcggct acgccgtttg ggcttccgag caggatccag cgcgcgctgt cgagttgttc
     6481 aggcaagcgg cgcgcatcga gccgcgcgat gcacgcctgc gctactacct ggcgctcgcg
     6541 ctcgaatcgg tcgccgactt ggacgcgacg cgccagtcgc tcgacgaagc gttggcgatc
     6601 gaccctcggc atatcgacgc gcttgcgatg ctgggccggc tggccctcga tgccggcgaa
     6661 ctcgagcgcg ccacggatct ggcggcccgc tgcatcgcgg cccggcccga ccatcccgcg
     6721 ggatggcagc tcaaggggca ggcggcgctg ttccgcatgg atgccgccga tgcggtgaag
     6781 agcttgcggc aggcgctgga ccttgctcct gacgatgtgg acacgcgcgt ctcgctcgcg
     6841 caggcggccc tgatgcaagg gcgaccgcga cacgccaaag atctgctgca gcaggcggtc
     6901 gccgagaacg cttcgcacga cacggcgctg tgcatgctcg gctgggcctg catcgcagac
     6961 aacgaagcgg ccggcgctgc ggcggccttc gagcgggcga acaccgccgc gcccgacaac
     7021 ccggaggcaa tggccggcat tgcattgctt cgtctggccg acgggcgacg cgatgaggcg
     7081 ctccaatgga ccgagcgcgc gctggcgctg gaaccggccc atgcgatggc cggcctgctc
     7141 agagcccgcc tcgccgagct tgacgatcgg cccgccgagg cgcgcaccgt cctcgcctcg
     7201 gtgctcgacg gggtgtcgag cgggcccttg ggggcatcga cgctcggaca aaagcttgca
     7261 gccgccggcg catcgccgac cgtggggcgc atgaagcgca gattcgctcg cgcgctgccc
     7321 gccgtaccga agaatcggtc gcgatagtgc gctgtcccgc tcgccattca actcactcgc
     7381 tgcccgtcat gaccctctct caagccaccg aactcgattc cgaggaactg ctgcaccttg
     7441 ccgttgccgc aagcgaccgc gaccaaaccg atcaggccat cggtttgctc aagagagcga
     7501 tcgatgtcga tccgtccaat gccagggcgc actatttgct gggtgccgaa catgcgcaga
     7561 tcggcctctt cgatcgcgcg gcccaggaga tgcggcgggc tctggaactc gacggagaac
     7621 tcgatgcggc gcgcttccaa ctgggcttgt tgctgctgac ctcgcgccag gtcgagccgg
     7681 ccgagtcggc atggaagcac ctggaccggc tgggaacggg acacttctac gtgctcttca
     7741 agaccgggct gctgtgcctc gcgaacgatg agttcagtga gagcctgcgc tgcctgcgcg
     7801 acggcatcac cgcgaaccag gagaacgccc cgttgaacgc ggacatgcag cggatcgctc
     7861 aacaagtcga agccatcctg gcgcagccgg gcgccgccgc gtcggcagac gccgaggact
     7921 cttccgcggc gcacctcctc atcaatgcct acacgggccg acggcactag ccccgagcgc
     7981 gggccgttcg gaccgttcca tggatcccgt atcgaaactg acgaagaccc tggcagcctt
     8041 tcgtcggatc accggatcgc ccgcgcctgc aggcggcaag gtgaagtcgc aagccggtca
     8101 ggctgcctcg gggcgaacgc cggcctcgac ggtcggagag ctgcatgcgg agatcgcccg
     8161 gcgcctcggc atggtcagtc cgctcgatcc gaatcggcgc gacgccgcgg tcaaggtgtt
     8221 tctcgaacag gtgctgctcc atgagtttgg gccgcagctg ctcacgtcgc agcggttcag
     8281 cgacatggtc cgcagcgtcc agcagaccat ggatgccgat ccggccgtca aggcgcagct
     8341 tgacgatctg atcggggaag tgtccgcgcg gtaatgatat gacgggtctg cggctactcc
     8401 ctgaccggcg cctgcgtcgc ctcccatgca atcctgggcg agcctgggcc cgataacgcg
     8461 cgatagggag acgccgaagg cgtgacaaag tgtcggaagg ccaacagctc cggcctacaa
     8521 gttcatgcct tctgcgtcat gcgcagccgc atcgatcttc gccttgcccc ggacgatggc
     8581 ctcccttgcg gattcgccac gcatccagca gtcaagcatg tccgcccaca cttgcatgag
     8641 gacccgcctc ggcccggaca agcgcgcatg gttgtaggct gcggcgatgt ggtccttctt
     8701 ctggtgggcc aggcaggctt cgatcaaggc ctccggaaag agggcgtcgt tgagccgaga
     8761 cgagaacgtt cggcgcaggt cgtggacgga gaccggcatg aatagcccct gatccggcgt
     8821 tcgatcctcg ttgatccgat ccaccgccgc ggcgatcgtc cggttcagcg tggcctggct
     8881 gatcggctcg gaggagtcat agcggctggg atggacgaag ctgctcgacg ggaagcaggt
     8941 tcgaagcgtt gtcaggatgt caagggcctg ctcggccagg tagaccgtgt gcggcttgcc
     9001 cgccttcatc cgatcggcag ggatcagcca ctgtgcccgt tcccagtcca cttcccccca
     9061 g
//
LOCUS       DATMJB010000260         2166 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_371741, whole genome shotgun sequence.
ACCESSION   DATMJB010000260 DATMJB010000000
VERSION     DATMJB010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371741"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="VNU48_05790"
     CDS             <1..148
                     /locus_tag="VNU48_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HWI10823.1"
                     /translation="QIGKINVNEFSTYVAVDRQIAAQALARLAAGKVKGRKVKVRLVQ
                     DERA"
     gene            223..1070
                     /locus_tag="VNU48_05795"
                     /pseudo
     CDS             223..1070
                     /locus_tag="VNU48_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384409.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
     gene            1212..1697
                     /locus_tag="VNU48_05800"
     CDS             1212..1697
                     /locus_tag="VNU48_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HWI10824.1"
                     /translation="MTSPANATPNPALRAVALFEAIKGLIVFGAGFGLLALLHHDVRR
                     VAEALVTRLHIDPEEHYAGIFLDAANHVTDAKLWALAVLAAAYTALRWAEAYGLWLNR
                     RWAAWLGAAGGAFYVPVELYELWHKPGPIKAATLLLNLALVAYLVWMLRQGRGAHAPT
                     A"
     gene            complement(1694..>2166)
                     /locus_tag="VNU48_05805"
     CDS             complement(1694..>2166)
                     /locus_tag="VNU48_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI10825.1"
                     /translation="AVITAARQAEAEEFIGELEDLYKRRGYDAHVGERGVKLSGGQRQ
                     RIAIARVFLKNAPILVLDEATSALDSEIEAAIQQSLGTLMAGKTVIAIAHRLSTIARM
                     DRLIVLDQGRIVEQGDHRGLLAQGGLYARLWAHQSGGFLGEEDDEPLLDDVAAG"
BASE COUNT          294 a          782 c          780 g          310 t
ORIGIN      
        1 gcagatcggc aagatcaacg tcaacgagtt ctcgacctat gtcgcggtcg accggcagat
       61 cgccgcgcag gcgctggcgc ggctcgcggc cggcaaggtc aagggccgca aggtcaaggt
      121 gcgcctggtg caggacgaac gcgcctgacg cgttcggtac cgacgaccgt gtggggcgcc
      181 ggcctacagc gccgatgcgc ggcgacattc acagtggcgc gcatgcagat cctttccagg
      241 ctggtggcgc tgtcgtccgc cgcggcgctc gcggcttgcg gcgatgtcgc gaccttgccg
      301 gtctctgccg gcaccggagc gaacccgacc ctgccggcgc cgcacaagac gctgctgccc
      361 accgtcaacg tcgcgccggc gcagggctgg gccgagggcg cgacgccggt ggccgcggcg
      421 ggcctgagcg tcaatgcgtt tgccaccggc ctcgatcatc cgcgcgggct gtatgtgctg
      481 cccaatggcg acgtgctcgt ggccgagacc aacgcgccgt acgcgacggt gggctcgaac
      541 agcaacgtcg gcgagaacgg cgtcgcggcc gaagagggcc gcgcggcgat ctggcagatc
      601 gatccgaaga ccggcaccca ccgcgtgttc gcggcgggcc cgcgcaaccc gaacgacatg
      661 gcctgggagc cgggcagcgg cgcgctgtgg acggtcgtga acgaacgcga cgagatcggc
      721 agcgacctcg tgcccgacta cctcacgtcg gtgcgcgacg gcggcttcta cggctggccg
      781 tacagccact acggagcgca cgtcgacgcg cgcgcgtcgc cgccgcgccc ggacctcgcc
      841 acgccaggcc cagcgacgcg gtgtgcggcc cgagcgcgca gtccggcgcg atcgcgttcg
      901 cgacggtcgc agggcacgag cctgccggcg cgcttcgcca gcggcatgtt cgtcggccag
      961 cacggctcgt ggaaccgccg gccgcacagc cggtcggcgt ggcgctcgac gcgaagggcg
     1021 cgctgctggt cgccgacgat gtcgccaacg tgatctggcg cgtcggcgcg gcgcgcttga
     1081 tccggcccgg cgcgcatcgc ggtcagcggc gcgcggctcg ccgagcgccc gcgtgcggcg
     1141 attgtcgagc gccgcggggc tgtgaaatga tcgcggcact ccgcgactct cacggctcgc
     1201 cacaacagcg catgaccagt cccgccaacg ccacaccgaa cccagccctg cgcgcggtcg
     1261 cgctgttcga ggccatcaag ggcctgatcg tcttcggtgc cggcttcggg ttgctggcgc
     1321 tgctgcacca cgatgtgcgc cgggtcgccg aggcgctggt gacccggctg cacatcgatc
     1381 ccgaggagca ctatgccggc atctttctgg acgcggccaa ccacgtgacg gacgcgaagc
     1441 tttgggccct ggccgtgctc gcggccgcct acaccgcatt gcgctgggcc gaggcctacg
     1501 gcctgtggct gaaccggcgc tgggccgcgt ggctgggcgc ggccggcggc gcgttctacg
     1561 tgccggtcga actctacgag ttgtggcaca agcccgggcc gatcaaggct gcgacgctgc
     1621 tgctgaacct cgcgctcgtc gcctacctcg tctggatgct gcgccagggc cgtggcgcgc
     1681 acgcgcccac ggcctaaccg gcggcgacgt cgtccagcag cggctcgtcg tcttcctcgc
     1741 cgaggaagcc cccgctctgg tgcgcccaca gccgcgcgta cagcccgccc tgcgcgagca
     1801 ggccgcgatg atcgccctgc tcgacgatgc ggccctggtc gagcacgatc aggcggtcca
     1861 tgcgcgcgat cgtcgagagc cggtgcgcga tcgcgatcac ggtcttgccg gccatgagtg
     1921 tgccgaggct ctgctggatc gcggcctcga tctcgctgtc gagtgcggag gtggcctcgt
     1981 cgagcaccag gatcggcgcg ttcttgagga acacgcgcgc gatcgcgatg cgctggcgct
     2041 gcccgcccga aagcttcacg ccgcgctcgc cgacatgcgc gtcgtagccg cggcgcttgt
     2101 agaggtcctc gagctcgccg atgaactcct cggcctcggc ctggcgcgcg gcggtgatca
     2161 ccgcct
//
LOCUS       DATMJB010000261         5192 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_702267, whole genome shotgun sequence.
ACCESSION   DATMJB010000261 DATMJB010000000
VERSION     DATMJB010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702267"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..382)
                     /locus_tag="VNU48_05810"
     CDS             complement(35..382)
                     /locus_tag="VNU48_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWI10826.1"
                     /translation="MKIIVRWLLLAAALLLVAYLYPGVTIKSFGSAMLAAFVLGLLNT
                     LLRPILVLLTLPVTLITLGLFLFVINALMFYFAAAMLDGFHVNGFTAALIGSLIYSLC
                     GMVIDVAMERVFA"
     gene            515..2845
                     /locus_tag="VNU48_05815"
     CDS             515..2845
                     /locus_tag="VNU48_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWI10827.1"
                     /translation="MIPFSSLVPLGALSAGIGLLVPPGFAQSADGAATRDRAQAAPAT
                     TLAAVTLAPVTVNGVAERDSNSVRATTSTMVKGHQDLRDIPQSVTVVTEKLIEDRRID
                     TLKEALHQTAGISFQAAEGGEEDIRLRGFSLAASGDIYVDGIRDPAFYERDTFSFDRI
                     ELLRGSASMLFGRGSTGGVVNQVSKQAFLANANQVDFTLGTGSYTRFTGDFNLKTNDT
                     TALRINVMTTNASGGGGSDKLDKQGLAPTFRWGIGTGDEFYVSGYYLRNDNGINYGLP
                     WLRKSSTQTSANPSGLIEGLDPRNYYGAASDYNAGGAALATVGHTHRFADGGTLETAI
                     RQGRYDRDQRASTVRFCQRTVNATTGVVSNPDCPTAIVAADTLSDATRLVRGTNNKVQ
                     DMTTTYAQSDYSNRFGWFGVKHEVLTGVDLAHEVFRNYAMVVPAGVTLNKNATTTTIG
                     TPDDGTGGVDESARLKRRSGAFDAKALGVYFQDLVQVAPHWKVLGGLRWDSFKGDYQT
                     FQTADGGIVVVGTQLAERSRSDSLWSKRFGVLYQPSDEMSFHLSYGTSFNTSGDTYQY
                     DDQTKNTPPEGSENVELGAKLDWLNGKLSTRVAAFYSIKTNERNRDPDSAATQALLSG
                     RRHAAGLEFDGAGRIAPGWELYASYAWTPVARIDVGAPGSVPGVGEGAGTRSALTPRH
                     SGTVWTTYQLTPALRLGGGLNARRAQTPNRNPAGINAPSFVTADLLGEYTLNEAVAFK
                     LNVNNLTNKLYADSLYTGHYVPGAGRSVQLTMTARF"
     gene            2863..3552
                     /locus_tag="VNU48_05820"
     CDS             2863..3552
                     /locus_tag="VNU48_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HWI10828.1"
                     /translation="MLLHIKEVLNADELREARAILARAPWGDGRATAGMQSALAKNNE
                     QLPQDGEPTKALQRIVLGGLNRHTLFFSAALPRRVFPPLFNRYGGTANAFGNHVDNAV
                     RFVPGGQGERVRTDVSCTLFLAEPDAYDGGELVVEDTFGPQRVKLPAGDMLLYPGTSV
                     HRVEPVTRGFRIASFFWIESMVRSAEARRLLFDMDAHLMRLRSSVGETDPAVIGLTGT
                     YHNLLRMWADA"
     gene            3608..3874
                     /locus_tag="VNU48_05825"
     CDS             3608..3874
                     /locus_tag="VNU48_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HWI10829.1"
                     /translation="MKRQLLTLCLTLAAAGAFAQHAEKCEPIPKEEWKPQAELERKLT
                     NQGWKISRVKITNGCYEVYGKNELGRNVELFFHPKTFELVTAPK"
     gene            3885..4427
                     /locus_tag="VNU48_05830"
     CDS             3885..4427
                     /locus_tag="VNU48_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HWI10830.1"
                     /translation="MSTPNVRVWDRAVRVLHWALVGAVALAALTLVVWVGAHQGAGYA
                     ALAIVALRALWGGVGGRYARPRQFVRAPRATLRYALAVVHGREPRYLGHNPLGGWMIV
                     ALMACITGLALTGWLYTTDAFWGDERVERVHRALAWTLLGLVALHVGGVLFTSLRQHE
                     NLVRAMLDGRKRAPSKDDIA"
     gene            4641..5036
                     /locus_tag="VNU48_05835"
     CDS             4641..5036
                     /locus_tag="VNU48_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HWI10831.1"
                     /translation="MATAKKAPAKKAAAKKAPAKKAAPAKKAAPAKKAAAKKAPAKKA
                     APAKKRTPNAAFMKAMTPSAALAAIIGAKPVPRTEVTKKVWEYIKANKLQDSAKRTMI
                     NADAKLKEIFKKAQVSMFEMTKLINAHLS"
BASE COUNT          948 a         1785 c         1728 g          731 t
ORIGIN      
        1 cctgtcgaag ggtcggcccg aacgggagtg gtcgctacgc gaacacccgc tccatcgcca
       61 cgtcgatcac catcccgcac aagctgtaga tcagcgagcc gatcagcgcg gcggtgaagc
      121 cgttgacgtg gaacccgtcg agcatcgccg cggcgaaata gaacatcagc gcgttgatca
      181 cgaacaggaa cagcccgagc gtgatcagcg tgaccggcaa ggtcagcagc accaggatcg
      241 gccgcagcaa ggtgttcaac aaaccgagca cgaaggccgc gagcatcgcc gagccgaaac
      301 tcttgatggt gaccccgggg tagaggtagg ccaccagcag cagcgcggcg gcgagcagga
      361 gccagcgaac gatgatcttc atcgccgcaa gaatagcgcg ccacggccgc gcgacgatgg
      421 ccgcttcgcc aatgcgagta ttaatcggaa tcattcgcat ttgagttagc atcgagaccg
      481 ttgcgggtca ccgcctcgac gggcttctca tcgcatgatt ccattcagtt cactcgtacc
      541 gctgggcgcg ctgtcggccg gcatcggcct gctggtgccg ccgggcttcg cgcaatcagc
      601 cgacggcgcg gcgacccgcg atcgcgcgca ggccgcgccg gcgacgacgc tcgccgcggt
      661 cacgctggcg ccggtcacgg tcaacggcgt ggccgagcgc gattccaact cggtacgcgc
      721 caccacctcg acgatggtca agggccatca ggatctgcgc gacatcccgc agtccgtcac
      781 cgtggtcacc gagaagctga tcgaagaccg ccgcatcgac acgctgaagg aggcgctgca
      841 ccagaccgcc ggcatcagct tccaggccgc cgagggcggc gaggaggaca tccgcctgcg
      901 cggcttctcg ctcgcggcca gcggcgacat ctatgtcgac ggcatccgcg acccggcgtt
      961 ctacgagcgc gacaccttca gcttcgaccg catcgagctg ctgcgtggct cggcctcgat
     1021 gctgttcggg cgcggatcga ccggcggcgt cgtgaaccag gtcagcaagc aggcgtttct
     1081 ggcgaatgcg aaccaggtcg acttcacgct cggcacgggc agctacacgc gcttcaccgg
     1141 cgacttcaac ctgaagacca acgacaccac tgcactgcgc atcaacgtga tgacgacgaa
     1201 cgcgagcggc ggaggtggca gcgacaagct cgacaaacag ggcctggcgc ccacgttccg
     1261 ctggggcata ggcaccggcg acgagttcta tgtctcgggc tactacctgc gcaacgacaa
     1321 cggcatcaac tacggcttgc cgtggctgcg caagagctcg acgcagacga gcgcgaaccc
     1381 gtccggcctg atcgaaggcc tggacccgag gaactactac ggcgccgcga gcgactacaa
     1441 cgccggcggc gcagcgctcg ccaccgtcgg ccacacgcat cgcttcgccg acggcggcac
     1501 gctcgagacg gcgatccgcc agggccgcta tgaccgcgac cagcgcgcca gcaccgtgcg
     1561 cttctgtcaa cgcacggtga acgcgaccac cggcgtggtc agcaacccgg attgcccgac
     1621 cgccatcgtc gcggccgaca ccctgagcga cgcgacccgg ctcgtgcgcg gcaccaacaa
     1681 caaggtgcag gacatgacga cgacctacgc gcagagcgac tacagcaacc ggttcggctg
     1741 gttcggcgtc aagcacgagg tgctgaccgg cgtcgacctc gcgcacgagg tgttccggaa
     1801 ctacgcgatg gtcgtgcccg ccggcgtgac gctgaacaag aacgcgacca ccacgacgat
     1861 cggcacgccc gacgacggca ccggcggggt cgacgaatcg gcgcggctga agcgccgctc
     1921 cggcgcgttc gacgcgaagg cgctcggggt gtattttcag gatctggttc aggtcgcgcc
     1981 gcactggaaa gtgctgggtg gcctgcgctg ggactccttc aagggcgact accagacctt
     2041 ccagaccgcc gacggcggca ttgtggtggt cggcacgcag ctcgccgagc gcagccgctc
     2101 cgacagcctg tggagcaagc gattcggcgt gctgtaccag cccagcgacg agatgtcgtt
     2161 ccacctctcg tacggcacct cgttcaatac ctccggcgac acctaccagt acgacgacca
     2221 gaccaagaac acgccgcccg agggcagcga gaacgtcgag ctcggcgcga agctcgactg
     2281 gctgaacggc aagctcagca cgcgcgtcgc ggccttctac agcatcaaga cgaacgagcg
     2341 caaccgcgac cccgacagcg ccgcgactca ggcgctgctg tccggccggc gccatgccgc
     2401 ggggctggaa ttcgacggcg ccgggcgcat cgcgccgggc tgggagctgt atgcgtcgta
     2461 cgcctggacg ccggtggcgc gcatcgatgt cggcgcgccc ggctcggtcc ccggcgtcgg
     2521 cgaaggcgcg ggcacgcgct cggcgctgac gccgcgccac agcggcacgg tctggacgac
     2581 ctatcagctc acgccggcgc tgcgcctggg cggcggcctg aacgcgcgcc gcgcgcagac
     2641 gccgaaccgg aatcccgccg ggatcaatgc gccgagcttc gtcaccgccg acctgctcgg
     2701 cgagtacacg ctgaacgagg cggtcgcgtt caagctcaac gtcaacaacc tgaccaacaa
     2761 gctctatgcc gattcgctct acaccggtca ctacgtgccg ggtgccggcc gctcggtgca
     2821 gctgacgatg accgcgcggt tctaggcccg acgagcgacg cgatgctgct ccatatcaaa
     2881 gaggtcctga acgccgacga gctgcgcgag gcgcgcgcga tcctcgcgcg tgcgccctgg
     2941 ggcgatgggc gtgcgaccgc gggcatgcag tcggcgctgg cgaagaacaa cgaacagctg
     3001 ccgcaggacg gcgagccgac gaaggcgctg cagcgcatcg tcctcggcgg gctgaaccgg
     3061 cacacgctgt tcttctcggc cgcgctgccc aggcgcgtgt tcccgccgct gttcaaccgc
     3121 tacggtggca ccgccaacgc gttcggcaac cacgtcgaca acgcggtgcg cttcgtcccc
     3181 ggtggccagg gcgagcgcgt gcgcaccgac gtgagctgca cgctgttcct ggccgagccc
     3241 gacgcgtacg acggcggcga actcgtcgtc gaggacacgt tcggcccgca gcgcgtcaag
     3301 ctgccggccg gcgacatgct gctgtacccc ggcaccagcg tgcaccgggt cgagccggtg
     3361 acgcgcggct ttcgcatcgc gagcttcttc tggatcgaga gcatggtgcg cagcgcagag
     3421 gcgcgccgcc tgctgttcga catggacgcg cacctgatgc ggctgcgcag ctcggtcggc
     3481 gagaccgacc ccgccgtgat cggcctgacc ggcacgtacc acaacctgct gcgcatgtgg
     3541 gccgacgcct gacgcgcggc gctgcgcttg ccctcgctca cctcatccac tcgaaaggaa
     3601 acctgcgatg aaacgccaac tgctgaccct gtgcctgacc ctggctgccg ccggcgcgtt
     3661 cgcgcagcac gccgagaagt gcgagccgat cccgaaggag gaatggaagc cgcaggccga
     3721 actcgagcgc aagctgacca accagggctg gaagatcagc cgcgtcaaga tcacgaacgg
     3781 ctgctacgag gtctacggca agaacgagct cggccgcaac gtcgagctgt tcttccaccc
     3841 gaagaccttc gagctcgtca ccgcgccgaa gtgacgcgcg cgccgtgagc acgccgaacg
     3901 tgcgcgtctg ggaccgcgcg gtgcgcgtgc tgcactgggc gctggtcggc gcggttgcgc
     3961 tcgccgcgct gaccctcgtg gtgtgggtcg gcgcgcacca gggcgccggt tatgcggcgc
     4021 tcgcgatcgt cgcgctgcgt gcgctgtggg gcggcgtcgg cggccgctac gcgcgtccgc
     4081 gacagttcgt gcgcgcaccg cgcgcgacgc tgcgctatgc gcttgcggtc gtgcacgggc
     4141 gcgagccgcg ctacctcggg cacaacccgc tgggcggctg gatgatcgtc gcgctgatgg
     4201 cctgcatcac cggcctcgcg ctgaccggct ggctctacac gaccgatgcc ttctggggcg
     4261 acgagcgcgt cgagcgcgtg caccgcgcgc tcgcgtggac cttgctcggg ctggtcgcgc
     4321 tgcacgtcgg cggcgtgctg ttcacgagcc tgcggcagca cgagaacctg gtccgggcga
     4381 tgctcgacgg ccgcaagcgc gcgccgtcga aggacgacat cgcttgatgt gatgacgccg
     4441 aaagtgcgta cgcggtgatg ccttgccacg acaaagcgcg tctcacctcg aagaattgcg
     4501 cgtttcacct gtgaaaactt cttctcttcc ctctatacag gcattaccat tcgcaccgca
     4561 cggtgctatg gagaaatgag agtccacgcg gctctcgccc atcccgggca catcaacaac
     4621 tcttgatgga gagaatcaac atggcaactg cgaagaaggc gccggctaag aaagcggcgg
     4681 cgaagaaggc cccggccaag aaggcggccc cggcgaagaa ggcggctccg gccaagaagg
     4741 ccgctgcgaa aaaggcgccc gcgaagaagg ccgctccggc caagaagcgc actcccaacg
     4801 ccgccttcat gaaggcgatg accccgagcg ccgcgctcgc cgcgatcatc ggcgccaagc
     4861 cggtgccgcg caccgaggtg acgaagaagg tctgggagta catcaaggcc aacaagctgc
     4921 aggactcggc caagcgcacg atgatcaacg ccgacgccaa gctgaaggaa atcttcaaga
     4981 aggcgcaagt ctcgatgttc gagatgacca agctgatcaa cgcgcacctg agctgaagcg
     5041 gcttcgtgca agaaaaaagg ccggtgcttg caccggcctt tttgttggcg cgtgccctat
     5101 ctcgtcgggc ccaggggtgt cggcggcgcc ggcgcgatcc agccggtcgc gagctgctgg
     5161 tacagctcgg ggccgaagcg cgcgctgatg gt
//
LOCUS       DATMJB010000262         5186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_8636, whole genome shotgun sequence.
ACCESSION   DATMJB010000262 DATMJB010000000
VERSION     DATMJB010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8636"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VNU48_05840"
     CDS             complement(<1..294)
                     /locus_tag="VNU48_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI10832.1"
                     /translation="MMDRVDAIVVGAGVVGLAIGRALALCGLETVVLERANAIGTGTS
                     SRNSEVIHAGIYYPTGSLKARLCVSGRELLYRYCESHGVEHRRCGKLLVATNEA"
     gene            complement(291..854)
                     /locus_tag="VNU48_05845"
     CDS             complement(291..854)
                     /locus_tag="VNU48_05845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006766717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HWI10833.1"
                     /translation="MRKVAVVVGSLRRESINLRFAKALSRLADGLMAFDITDLSDVPM
                     YNEDLWHQPPAGVLRLKRELDAADAVLFVTPEYNRSIPAVTKNVVDWGSRPYGQNSWA
                     GKPGAIVGTSPGAIGTAVAQAQLRSILVGCGVNLLGQPEVYFQTKPGLIADDFSTADD
                     KTRAFLAGFVAKFADHIERLAARPSPP"
     gene            complement(887..1864)
                     /locus_tag="VNU48_05850"
     CDS             complement(887..1864)
                     /locus_tag="VNU48_05850"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWI10834.1"
                     /translation="MLVPFLGLSACGGGGGGGSAAATDTAPSVSFAFNCSDLSCTFTS
                     TSSDQDAGDTLTYSWNFGDGATATTANASHPYTAAGTYTVSLSATDNHGVPGNPNPAT
                     AQVTVTAPPTPAGTHANFSVSCAALTCTFNDTSTFGGGATLQSRAWDFGDSTSATTNP
                     AVHAYTATAPTTYTVKLTVTDSLGKVSTSIQSIPVVPASTALLTLTQKSTVTATFVST
                     SCTAHNNQVVITAPITQTILTDGCFDQAQLGTVVPINAGSAFAANTVLQVDVLSGLSG
                     TTALVFPPTIRVTGDFASGWTLTFDDGFGGPGEPDFNDLVIKITATPSP"
     gene            complement(2016..2090)
                     /locus_tag="VNU48_05855"
     tRNA            complement(2016..2090)
                     /locus_tag="VNU48_05855"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2056..2058),aa:Val,seq:cac)
     gene            complement(2140..2856)
                     /gene="dnaQ"
                     /locus_tag="VNU48_05860"
     CDS             complement(2140..2856)
                     /gene="dnaQ"
                     /locus_tag="VNU48_05860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HWI10835.1"
                     /translation="MRQIFLDTETTGLSPESGDRIIEIGCVEMLNRRLSGRDLHFYLN
                     PQRRSHEDAVKVHGLSDEFLADKPLFASIVDELLAFVDGAEIIIHNASFDVGFLNEEL
                     RRLDRPKFPGFVARITDSLSMAREMFPGKANSLDALCRRLEVDNSNRTLHGALLDAGL
                     LAEVYIRMTRGQNSLVIDGGETSAAQAALEAVDFARLDLRVVHADEAEAAAHELVLAE
                     LDKASGGRTIWRQLEATAVA"
     gene            complement(2866..3687)
                     /gene="lgt"
                     /locus_tag="VNU48_05865"
     CDS             complement(2866..3687)
                     /gene="lgt"
                     /locus_tag="VNU48_05865"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWI10836.1"
                     /translation="MTIAYPQFNPVALQIGPVAIHWYGLTYLVAFVLFIWLGAKRVQL
                     AQFAARGWSRRDIEDLLFYGVLGVVLGGRLGYVLFYKPGYYGAHPLEIFAVWKGGMSF
                     HGGMLGVFVAIALFARSRGRQFLDATDLIAPCVPLGLASGRIGNFINGELPGRLADPG
                     LPWAMVYPNAGPLPRHPSSLYQFALEGVLLFVLLWIYARKPRGLGQVSGAFLVGYGVL
                     RFTAEYFREPDDFLGLLALNLSMGQWLCVPMIIAGVAMWLWGRGREPRGAAGSAS"
     gene            complement(3723..4688)
                     /locus_tag="VNU48_05870"
     CDS             complement(3723..4688)
                     /locus_tag="VNU48_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWI10837.1"
                     /translation="MRDSDPANADAGIELRVWRQFLTLADTLHFGRAAGLLHITQPPL
                     TQAIQQLERRLGTRLFERTRRSVALTPAGALMVEPVRQLLRQAATLQTRARDAAAGEL
                     GRVRVGFISTVGFGPLPLWLRAFRQQQPGIAIELIEATSDVQLKALGRAEIDVGFMLH
                     APGLAPTAPMPLQRLSLGVEPLVLALPDTAAWAAPRRLRPLDVLAQPLVIFPRAATPS
                     LYDAVLAFYHRHGVTPAIAQEATQMQTIVNLVSAGLGLALVPEVVTQLQRPGVVYRPV
                     PAALADGAPRCETSLLWPDAPAPAVARFVEFVRALPVRPLRYRSR"
     gene            4779..>5186
                     /locus_tag="VNU48_05875"
     CDS             4779..>5186
                     /locus_tag="VNU48_05875"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017403811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWI10838.1"
                     /translation="MTDALKINRRSAHITEGVARAPNRSMYYAMGYEEGDFRKPMIGV
                     ANGHSTITPCNSGLQKLADAAVAGIEAAGGNAQIFGTPTISDGMAMGTEGMKYSLVSR
                     EVIADCIETCVGGQWMDGVLVVGGCDKNMPGGMM"
BASE COUNT          836 a         1744 c         1830 g          776 t
ORIGIN      
        1 ggcctcgttc gtcgccacca gcagcttgcc gcagcggcgg tgctcgacgc cgtgcgattc
       61 gcagtagcgg tacagcagct cgcggccgct cacgcacagc cgcgccttca gcgagcccgt
      121 cggatagtag atgccggcgt ggatgacctc gctgttgcgc gaactggtgc cggtgccgat
      181 cgcattcgcg cgttcgagca cgacggtttc gagcccacac aacgccagcg cgcgcccgat
      241 cgcgaggccg acgaccccgg cgccgacgac gatcgcgtcg acgcggtcca tcagggcggc
      301 gaaggccgag ccgcgaggcg ttcgatgtgg tcggcgaact tggcgacgaa gccggcgaga
      361 aacgcgcggg tcttgtcgtc ggcagttgaa aagtcgtccg cgatcaggcc gggcttggtc
      421 tggaaataga cctcgggttg gccgagcagg ttgacgccgc agcccaccag aatgctgcgc
      481 agctgggcct gagcgaccgc ggttccgatc gcgcccgggc tggtgccgac gatcgcgccg
      541 ggcttgccgg cccaggagtt ctggccgtag ggccgcgagc cccagtcgac cacgttcttg
      601 gtcaccgccg ggatcgagcg gttgtactcg ggcgtgacga acagcaccgc atcggccgca
      661 tcgagttcac gcttgaggcg cagcacgccg gccggcggct ggtgccacag gtcttcgttg
      721 tacatcggca cgtcgctcag atcggtgatg tcgaaggcca tcaggccgtc ggcgagcctg
      781 gacagcgcct tcgcgaacct caggttgatg gattcgcggc gcagactgcc gacgacgacc
      841 gcgaccttgc gcatgacttc gctgttctgc ctcgcagcgg ccgactttac ggcgacggag
      901 tggccgtgat cttgatgacc aggtcgttga agtcgggttc tccgggaccg ccgaacccgt
      961 cgtcgaaggt cagcgtccag ccactggcaa agtcgccggt gacgcggatc gtcggcggga
     1021 acaccagcgc cgtggtcccg gacaggcccg acaacacgtc cacctggagc accgtgttgg
     1081 cggcgaacgc gctgcctgcg ttgatcggta ccaccgtgcc cagctgggct tggtcgaagc
     1141 agccgtcggt caggatcgtc tgtgtgatcg gcgccgtgat cacgacctgg ttgttgtgcg
     1201 cggtgcagga cgtgctcacg aaggtggcgg ttaccgtcga cttctgcgtg agggtgagca
     1261 gcgccgtcga cgccggtacc accggaatgc tttgaatgct cgtgctgacc ttgcccaagc
     1321 tgtcggtgac ggtcagcttg accgtgtacg tcgtcggcgc cgtggcggtg tacgcgtgga
     1381 cggctggatt cgtggtggcc gacgtgctgt caccgaaatc ccaggcccgc gactggagcg
     1441 tcgcgccgcc accgaaggtg ctggtgtcgt tgaaggtgca ggtcagcgcg gcgcaactga
     1501 cgctgaagtt cgcgtgcgtc ccggccgggg tcggcggtgc agtcacggtg acttgagccg
     1561 tcgcgggatt cggattgccg ggaacaccgt ggttgtcggt ggccgacagg ctgacggtgt
     1621 acgttcctgc ggccgtgtag gggtggctgg cgttcgccgt cgtggccgtg gcgccgtcgc
     1681 cgaagttcca ggagtaggtg agcgtgtcgc cggcgtcctg atcgctgctg gtgctcgtga
     1741 aggtgcagct gagatcgctg cagttgaacg cgaagctcac gctcggcgcg gtatcggtcg
     1801 cagcagcgga tccaccaccg ccgccgcctc cgcacgcgga caagccgagg aacgggacca
     1861 gcatcaacgc gcgcagccag ctgtgtgtca tgtcggggca ccagtagaaa gtaggggctg
     1921 gagcgagggt cgcctccagc gagttcgtac tttaccggct gtgtgacagt ggcgccgcca
     1981 gacgcgtaca aatttcgcgc ggcgcgggcc ttcgatggtg ggcggtgagg gtttcgaacc
     2041 cccgacccca acagtgtgaa tgttgtgctc tacccctgag ctaaccgccc gaatttcgct
     2101 gttcggccgc gcctcgggga ggcgccgaac agccttggat tatgccacag cggttgcttc
     2161 caattgccgc cagatcgtgc ggccgccgct cgccttgtcc agctcggcca gcaccagttc
     2221 gtgggccgcc gcttcggcct cgtcggcgtg cacgacgcgc aagtccagcc gggcgaaatc
     2281 gaccgcttcg agcgcggcct gcgcagcact cgtttcgcca ccatcgatga ccagcgaatt
     2341 ctggccgcgc gtcatgcgga tgtagacctc ggccagcagc ccggcgtcga gcagcgcgcc
     2401 gtgcagcgtg cggttcgagt tgtcgacctc caggcgccgg cacagcgcgt cgagcgagtt
     2461 cgccttgccc gggaacatct cgcgcgccat cgacaggctg tcggtgatgc gcgcgacgaa
     2521 gcccggaaac ttcggccggt cgagccgccg cagttcctcg ttcaggaagc cgacgtcgaa
     2581 gctcgcgttg tggatgatga tctcggcgcc gtcgacgaac gcgagcagct cgtcgacgat
     2641 gctcgcgaac aagggcttgt cggccaggaa ctcgtcgctc agcccgtgga ccttgaccgc
     2701 gtcctcgtgg ctgcgccgct gcgggttgag gtagaagtgc aggtcgcggc ccgacaggcg
     2761 ccggttcagc atctcgacgc acccgatctc gatgatgcgg tcgcccgatt cggggctcag
     2821 gccggtggtc tcggtgtcga gaaagatctg gcgcatcggc gaattctagg aggcggaacc
     2881 ggcggcgccg cgcggctcac ggccccggcc ccagagccac atcgcgacgc cggcgatgat
     2941 catcggcacg cacagccact gccccatgct caggttcagc gcgagcagcc ccagaaagtc
     3001 gtcgggttct cggaaatatt cggccgtgaa gcgcagcacg ccgtagccga ccaggaacgc
     3061 gcccgagacc tggcccaggc cgcgcggctt tctcgcgtag atccacagca gcacgaacag
     3121 cagcacgccc tcgagcgcga actggtacag cgacgacggg tgccgcggca gcggcccggc
     3181 gttcggatag accatcgccc acggcaggcc cgggtcggcg agccgcccgg gcagttcgcc
     3241 gttgatgaaa ttgccgatgc gccccgacgc caggcccagc ggcacacagg gcgcgatcag
     3301 gtcggtcgcg tcgaggaact ggcggccgcg gctgcgcgcg aacagcgcga tcgcgacgaa
     3361 cacgcccagc atgccgccgt ggaacgacat gccgcccttc cagaccgcga agatctccag
     3421 cggatgcgcg ccgtagtagc ccggcttgta gaacagcaca tagcccagcc ggccaccgag
     3481 cacgacgccg agcacgccgt agaacagcag gtcctcgatg tcgcggcgcg accagccgcg
     3541 cgcggcgaac tgcgcgagct gcacacgctt cgcgccgagc cagatgaaca gcacgaacgc
     3601 gaccaggtag gtcagcccgt accagtggat cgcgaccggg ccgatctgca gcgcgaccgg
     3661 gttgaactgg gggtaggcaa tcgtcatcgg cgtgttcgtc gtttgccggc gcggcggccg
     3721 gatcagcgcg aacgataacg cagcggccgc accggcagcg cgcgcacgaa ctcgacgaag
     3781 cgcgcgaccg ccggcgccgg cgcgtcgggc cacagcaggc tggtctcgca acgcggtgcg
     3841 ccgtccgcga gcgcggccgg cacgggccgg tagacgacgc cgggccgctg cagctgcgtg
     3901 acgacctcgg gcaccagcgc cagccccagg ccggccgaga cgaggttgac gatggtctgc
     3961 atctgcgtcg cctcctgcgc gatggccggt gtgacgccat gccggtggta gaaggccagc
     4021 accgcgtcgt acagcgacgg cgtcgcggcg cgcgggaaga tcaccagcgg ctgcgccagc
     4081 acgtcgagcg gccgcagccg gcgcggcgcg gcccaggccg ccgtgtctgg cagtgccagc
     4141 accagcggct cgacgccgag cgacagccgc tgcaacggca tcggcgcggt cggcgcgagc
     4201 ccgggcgcgt gcagcatgaa gccgacatcg atctcggcgc gcccgagggc cttcagctgc
     4261 acgtcgctgg tcgcctcgat cagctcgatc gcgatgcccg gttgctgctg ccgaaaggcg
     4321 cgcaaccaca gcggcagcgg cccgaagccg acggtcgaga tgaagcccac gcgcacccgg
     4381 ccgagctcgc ccgccgcggc gtcgcgggcg cgcgtctgca gggtcgcggc ctggcgcagc
     4441 agctgccgca ccggctcgac catcagcgcg ccggccggcg tcagcgcgac gctgcggcgg
     4501 gtgcgctcga acaggcgcgt gcccaggcgc cgttcgagct gctggatcgc ctgcgtcagc
     4561 ggcggctgcg tgatgtgcag cagccccgcg gcgcggccga agtgcagcgt gtcggcgagc
     4621 gtcaggaact ggcgccagac gcgcagttcg atgcccgcat cggcgttcgc cgggtcggaa
     4681 tctctcattc gccaagcata tcaatccccg cctggatatc gattggacgc aacccgcgcc
     4741 agcccggata ctgtgcgctc ccgctctgga gaccgaccgt gaccgacgcc ctgaagatca
     4801 accgccgttc cgcccacatc accgagggcg tggcccgcgc gccgaaccgc tcgatgtact
     4861 acgcgatggg ctacgaggaa ggcgacttcc gcaagccgat gatcggcgtc gccaacggcc
     4921 actcgaccat cacgccctgc aactcgggat tgcagaagct cgccgacgcc gccgtggccg
     4981 gcatcgaagc cgccggcggc aacgcgcaga tcttcggcac gccgacgatc agcgacggca
     5041 tggcgatggg caccgagggc atgaagtaca gcctggtctc gcgcgaggtg atcgccgact
     5101 gcatcgagac ctgtgtgggc ggccagtgga tggacggcgt gctggtggtg ggcggctgcg
     5161 acaagaacat gcccggcggc atgatg
//
LOCUS       DATMJB010000263         2261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_406299, whole genome shotgun sequence.
ACCESSION   DATMJB010000263 DATMJB010000000
VERSION     DATMJB010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406299"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VNU48_05880"
     CDS             complement(<1..651)
                     /locus_tag="VNU48_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWI10839.1"
                     /translation="MRLQLLSDLHLETELFEPAPAPDAELLVLAGDIDSRWAGLEQFR
                     GWPVPVLFVAGNHEFDRRELTQAWPQLRERCARLGLRLLECESLVLTDRSGRRIRFVA
                     TIRWSDFDLFGTAQRERAVRAGGYFVNIMRATRGGRPLDAAAVRAEALACGAWLRSEL
                     ERPRGAWDATVAITHFAPSLRSVDPRYGAQPSSASFCNADDALLPHAELWIHGHLHC"
     gene            complement(678..1454)
                     /locus_tag="VNU48_05885"
     CDS             complement(678..1454)
                     /locus_tag="VNU48_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HWI10840.1"
                     /translation="MPTKDLPPSTAARAHDLTDAEFAELDDLLLETPEPLEPLDAVML
                     DGYLCGVIVQPVLLEPATWLAHVFDFDGRPLPDDVKPEWLQRTSALIMRRHAALNRAI
                     VEDGWFDPLVLEFDDEHPREPVPEGEVDPMAGLSPISQSLMPWVAGFQHATVCFPDLA
                     EMPDDAVMSALARLYRHLPTETAEEREVVQTLDREHPLATLDDAMEELVVTVADLQDL
                     TNEQRYKVETVKRESPKVGRNDPCPCGSGKKFKQCHGSAA"
     gene            complement(1499..2221)
                     /gene="hutC"
                     /locus_tag="VNU48_05890"
     CDS             complement(1499..2221)
                     /gene="hutC"
                     /locus_tag="VNU48_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HWI10841.1"
                     /translation="MTAPPTASRTLPYATVKAALRERIAQGGWEPGVRLPSERELVQA
                     FGCARMTVHRALRELEEEGLIERRQGSGSYVAELHPISNLLQVRDIHDEIRERGHAHT
                     TRVVSVARDRADARTAAAMRLRKGAPVYRVQLVHLENGVPIQLEDRHVNPALAPDLMA
                     RDFTQVTPSFVLFEHAPLTEAEQVVEAVLASIDQARLLDVAAGSALLMVSRRTVSQGA
                     VASVARLYHPGARYRLIGHFAV"
BASE COUNT          318 a          788 c          831 g          324 t
ORIGIN      
        1 acagtgcagg tgcccgtgga tccacagctc ggcgtgcggc agcagcgcgt cgtcggcgtt
       61 gcagaagctc gcgctgctgg gctgcgcgcc atagcgcggg tcgacgctgc gcaggctcgg
      121 cgcaaagtgc gtgatcgcca ccgtcgcgtc ccacgcgccg cgcgggcgtt cgagctcgct
      181 gcgcagccag gcgccgcagg cgagcgcctc ggcgcgcacc gccgccgcgt ccagcggacg
      241 gccgccgcgc gtggcccgca tgatgttgac gaaatagccg ccggcgcgca ccgcgcgctc
      301 gcgctgcgcc gtgccgaaca gatcgaaatc gctccagcgg atcgtcgcga cgaagcggat
      361 gcgccggccg ctgcgatcgg tcagcaccag gctttcgcat tcgagcaggc gcaggccgag
      421 gcgcgcgcag cgctcgcgca gctgcggcca ggcctgcgtc aactcgcgcc ggtcgaactc
      481 gtggttgccg gcgacgaaca gcaccggcac cggccagccg cgaaactgct cgagcccggc
      541 ccagcggctg tcgatgtcgc cggcgagcac cagcagctcg gcatcgggcg ccggtgccgg
      601 ctcgaacagt tcggtttcga ggtgcaggtc ggagagcagc tgcagccgca tcgatcagtc
      661 cggcgcgcgg cgccgggtca ggcggcagag ccgtggcact gcttgaactt cttgccgctg
      721 ccgcacgggc acgggtcgtt gcggccgacc ttcggcgact cgcgcttcac ggtctcgacc
      781 ttgtagcgct gctcgtttgt caggtcctgc aggtcggcga cggtgacgac gagttcctcc
      841 atcgcgtcgt cgagcgtggc gagcgggtgc tcgcggtcca gcgtctgcac gacctcgcgc
      901 tcctcggcag tctcggtcgg caggtgacgg tacagccgcg ccagcgccga catcaccgcg
      961 tcgtcgggca tctcggcgag gtcggggaag cagacggtgg cgtgctggaa accggcgacc
     1021 cagggcatca gcgattgcga gatcgggctc aggccggcca tcgggtcgac ctcgccctcg
     1081 ggcaccggct cgcgcgggtg ctcgtcgtcg aactccagca ccagcgggtc gaaccagccg
     1141 tcctcgacga tcgcgcggtt cagcgcggca tggcggcgca tgatcagcgc gctcgtgcgc
     1201 tgcagccact cgggcttgac gtcgtcgggc agcgggcggc cgtcgaagtc gaacacatgg
     1261 gcgagccagg tggcgggctc gagcagcacc ggctggacga tcacgccgca caggtagccg
     1321 tcgagcatca ccgcgtccag cggctccagc ggctcggggg tttcgagcag cagatcgtcg
     1381 agttcggcga actcggcgtc ggtcaggtcg tgggcgcgcg cagcggtgga gggcggaagg
     1441 tctttggtcg gcatggcggg gctcggcgca tcgggacgcc attgtgaccg cggcctgtct
     1501 atacggcgaa gtggccgatc aaacgatagc gcgcgccggg gtgatacagc cgcgcgaccg
     1561 acgcgaccgc gccctgcgag acggtgcggc gcgagaccat cagcagcgcg ctgccggcgg
     1621 cgacatcgag cagccgcgcc tgatcgatgc tcgccagcac cgcctcgacg acctgctcgg
     1681 cctcggtcag cggcgcatgc tcgaacagca cgaacgacgg cgtgacctgc gtgaagtcgc
     1741 gcgccatcag gtcgggcgcc agggccgggt tcacgtgccg gtcctcgagc tggatcggca
     1801 cgccgttttc caggtgcacg agctgcaccc ggtagaccgg cgcgcccttg cgcagccgca
     1861 tcgcggcggc ggtgcgcgca tcggcccggt cgcgcgcgac gctgacgacg cgcgtcgtgt
     1921 gggcgtggcc gcgttcgcgg atctcgtcgt ggatgtcgcg cacctgcagc aggttcgaga
     1981 tcgggtgcag ctcggccacg tacgagcccg agccctggcg gcgctcgatc aggccctctt
     2041 cttcgagctc gcgcagcgcc cggtgcacgg tcatgcgcgc gcaaccgaac gcctgcacca
     2101 gttcgcgctc cgacggcagc cgcacgccgg gctcccagcc gccctgcgcg atgcgctcgc
     2161 gcagcgcggc cttcacggtg gcgtaaggga gcgtgcggga tgcggtcgga ggcgcggtca
     2221 tcggctcatt ctagcgacgc ccatgctaaa gttgtctaga c
//
LOCUS       DATMJB010000264         2580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_76885, whole genome shotgun sequence.
ACCESSION   DATMJB010000264 DATMJB010000000
VERSION     DATMJB010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76885"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2424
                     /locus_tag="VNU48_05895"
     CDS             <1..2424
                     /locus_tag="VNU48_05895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HWI10842.1"
                     /translation="TYRYLPLGLPRGTRNYLERAIQGGKIASANFHVKGDLADFPFFD
                     AKPGQDGEFRIGAKIEDANFAFVPSVPADAGTPAYESPWPALSNLNVELLLERGTLEL
                     RNGRTQLGGVDWPKVQVAIHHLDADPVLTLDAAGRGPLAEMLRIVNTSPIGGWLDKAL
                     ASSTASGAADLSLNLGIPLKNVPATTVKGSVVLAGNDLRIAADSPLLAGARGRVDFSN
                     KGFAVVGGGARIYGGDASFDGGSQPGGAVRFNGQGTVSADGLRRSTELGALARAAGAL
                     AGQTAYRASLEFARGQSELTITSNLVGLAINLPAPLAKAAEAPLALRLSTSGDATAPA
                     TAPRDTLRFELGNVLRAQYVRDLSGERPRVLRGGVGVMEAAPEPASGVAASINLSRLV
                     TDEWEAASDKLLGSGQGGDATAASSAYLPDAIGLRVQELIAGPRRLTHVSAGLSQDAG
                     VWRANVDADQLNGYIAYRVPLGRGGAATAGGRVYARLSRLSLPKGDDAQVESLLDQQP
                     AALPALDVVVDEFELRGRQLGRVEIEAINRAGALGRDWQLSKFNITTPEAQLTATGHW
                     AAVGAPTLRGAPSPRRAVMDFKLQIADSGALLARLGTPKAVRGGKGQLSGQIAWQGSP
                     FALDYPSLAGQVNVAVDSGQFLKVEPGAARLLGVLSLQSLPRRLSLDFRDLFQEGFAF
                     DNLTGDVTIAQGVAKTNNLRMRGVQALVLMEGSADIEHESQDLRVVVVPEINAGTAAL
                     AYAVINPAVGLGAFLAQAILKKPLTAAGTREFHISGPWADPKVERTERRLGDDVASPE
                     AAASTPAKK"
     gene            2447..>2580
                     /locus_tag="VNU48_05900"
     CDS             2447..>2580
                     /locus_tag="VNU48_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWI10843.1"
                     /translation="MKIAALQMVSTTRVEANLDTARRLIAEAAHAGAALVALPEYFCL
                     "
BASE COUNT          359 a          936 c          953 g          332 t
ORIGIN      
        1 acctatcgct acctgccgct gggtctgccg aggggcacgc gcaactacct cgagcgcgcg
       61 atccagggcg gcaagatcgc cagcgcgaac ttccacgtca agggcgacct ggcggacttt
      121 ccgttcttcg acgcgaagcc cggccaggac ggcgagttcc gcatcggcgc gaagatcgag
      181 gacgccaact tcgcgttcgt gccgagcgtt ccggccgacg ccggcacgcc ggcctacgag
      241 tcgccgtggc cggcgctgag caacctgaac gtcgagctgc tgctcgagcg cggcacgctg
      301 gagctgcgca acggccgcac ccagctcggc ggtgtcgact ggcccaaggt gcaggtcgcg
      361 atccatcatc tcgatgccga tccggtgctg acgctcgatg ccgccggccg cggcccgctc
      421 gccgagatgc tgcgcatcgt caacacctcg cccatcggcg gctggctcga caaggcgctc
      481 gcgtcgagca cggccagcgg cgcggccgac ctgagcctga acctgggcat cccgctgaag
      541 aacgtgccgg ccacgaccgt gaagggcagc gtcgtgctgg ccggcaacga cctgcgcatc
      601 gcggccgaca gcccgctgct ggccggcgcg cgcggccgcg tcgatttctc gaacaagggc
      661 ttcgcggtgg tcggcggcgg cgcccggatc tacggcggcg atgcgagctt cgacggcggt
      721 tcgcagcccg gcggcgcggt gcgcttcaac ggccagggca ccgtcagcgc cgacggcctg
      781 cggcgctcga cggaactcgg cgcgctggcg cgcgccgccg gcgcgctcgc cgggcagacc
      841 gcgtaccgcg cgagcctgga gttcgcgcgc ggccagtcgg agctgaccat caccagcaac
      901 ctcgtcggcc tcgcgatcaa cctgccggcg ccgctggcga aggccgccga ggcaccgctg
      961 gcgctgcgcc tgtcgaccag cggcgatgcc acggcacccg ccaccgcgcc gcgcgacacg
     1021 ctgcgcttcg agctcggcaa cgtgctgcgg gcgcagtacg ttcgcgacct ttcgggcgag
     1081 cggccgcgcg tgctgcgtgg cggggtcggc gtgatggagg cggcgcccga gccggccagc
     1141 ggcgtggcgg ccagcatcaa cctgagccga ctcgtcaccg acgagtggga ggccgccagc
     1201 gacaagctgc tgggcagcgg ccagggcggt gacgccaccg cggcgtcgtc ggcctacctg
     1261 ccggacgcga tcggcctgcg cgtgcaggag ctgatcgccg gcccgcggcg cctgacgcac
     1321 gtctcggccg gcctgtcgca ggacgccggg gtctggcgcg ccaacgtcga cgccgaccag
     1381 ctcaacggct acatcgcgta ccgcgtgccg ctcgggcgcg gcggcgcggc gacggccggc
     1441 gggcgcgtct acgcgcggct gtcgcgcctg tcgctgccca agggcgacga tgcgcaggtc
     1501 gagagcctgc tcgaccagca gcccgcggcg ctgccggcgc tcgacgtcgt cgtcgacgag
     1561 ttcgaactgc gcggcaggca gctcggccgg gtcgagatcg aggccatcaa ccgcgccggt
     1621 gcgctgggcc gcgactggca gctgtcgaag ttcaacatca ccacccccga ggcgcagctg
     1681 accgcgaccg ggcactgggc cgcggtcggc gcgccgacgc tgcgcggcgc gccgagcccg
     1741 cggcgcgcgg tgatggactt caagctgcag atcgccgaca gcggcgcgct gctcgcgcgc
     1801 ctgggcacgc ccaaggccgt gcgcggcggc aaggggcagc tctcggggca gatcgcgtgg
     1861 cagggctcgc cgttcgcgct cgactacccg agcctggccg ggcaggtcaa cgtcgcggtc
     1921 gactccggcc agttcctgaa ggtcgagccg ggcgcggccc ggctgctcgg cgtgctgagc
     1981 ctgcagtcgc tgccgcgccg gctgtcgctg gactttcgcg acctgttcca ggaaggcttc
     2041 gcgttcgaca acctgaccgg cgacgtgacg atcgcgcagg gcgtggccaa gaccaacaac
     2101 ctgcgcatgc gcggcgtgca ggcgctggtg ctgatggagg gcagcgccga catcgagcac
     2161 gagagccagg acctgcgcgt cgtcgtcgtg cccgagatca acgccggcac cgcggcactc
     2221 gcctatgcgg tgatcaaccc ggccgtcggg ctgggtgcgt tcctcgcgca ggcgatcctg
     2281 aagaagccgc tgacggccgc cggcacgcgc gagttccaca tcagcgggcc gtgggcggat
     2341 ccgaaggtcg agcgcaccga gcgcaggttg ggcgatgatg tggcgtcgcc cgaggccgct
     2401 gcctcgaccc cggcgaagaa gtgatgacga cgttcgcgag gcctgcatga agatcgccgc
     2461 gctgcagatg gtgtccacga cccgcgtcga ggccaacctc gacacggccc ggcggctgat
     2521 cgccgaggcg gcgcatgccg gcgcggcgct cgtggcgctg cccgagtact tctgcctgat
//
LOCUS       DATMJB010000265         5424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_541635, whole genome shotgun sequence.
ACCESSION   DATMJB010000265 DATMJB010000000
VERSION     DATMJB010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541635"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VNU48_05905"
     CDS             complement(<1..704)
                     /locus_tag="VNU48_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008343473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWI10844.1"
                     /translation="MNNRRTFLRRAGASALGAAAVSRAGAATLPEAPMQVSTTMQPPP
                     APASGRPYHPVVTLNGWSLPWRMKDGVKEFHLVAEPVLRELAPGMAANLWGYNGASPG
                     PTIEAVEGERVRIFVTNKLPEATSIHWHGLLVPSGMDGVTGLTQPGIAPGKTFVYEFA
                     LARSGTYMYHPHADESLQMAMGLMGLFIVHPKDPREHRVDRDFALLLAAYDIDPGSAT
                     PRVNTMLDFNLWTWNSR"
     gene            complement(701..2089)
                     /locus_tag="VNU48_05910"
     CDS             complement(701..2089)
                     /locus_tag="VNU48_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWI10845.1"
                     /translation="MKNPFVPSAAARACSVALLALAQVGCASFSPDGGFGKVAELTRQ
                     RTGQTASWQRSPADVDAAQARVAELLAQPLNADRAVELALLNNRGLQASFAELGIAES
                     ELVRVGRLPNPSLAFAHLRGGGVTEIDRSMLFNVLALLTLPAAHEVEQQRFEQTQYQA
                     ALDAVQVAAQARRAFYSAVAAGELATYAVQVKDAADASGELARRMLQAGNFNRLAQLR
                     QQAFQADASAQLVRAQHQAVVERERLTRLLGLQGDQLKFRLPERLPDLPDAPAEAQDA
                     EQAALDQRLDVLIAKRSAQATAQQLGLTNATRFVNVLELGYANKSQTGEAGRSGYQIE
                     LELPLFDFGSVRNARAEAVYMRSVQHAAEVAINARSQVRESYAAYRSSYELARHCRDE
                     VLPLRKRISDENLLRYNGMLIGVFELLADAREQVASVTTCVEALRDHWLAQSQLQAAL
                     TGGDTEPSKASR"
     gene            complement(2193..2561)
                     /locus_tag="VNU48_05915"
     CDS             complement(2193..2561)
                     /locus_tag="VNU48_05915"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HWI10846.1"
                     /translation="MKTLRLCLLVLLAVLLPIRGAVAAAMPCASTGMHHESGAVAMHA
                     HHAGMAASGHDPAAHGEHHAGGAPTCNLCCDFCSMATLPGSPAALPLATNFATLSFPD
                     LFAPLPNFLSDGQERPPRST"
     gene            complement(2604..3062)
                     /gene="cueR"
                     /locus_tag="VNU48_05920"
     CDS             complement(2604..3062)
                     /gene="cueR"
                     /locus_tag="VNU48_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HWI10847.1"
                     /translation="MEKQPMNIGEAAKASGVSAKMIRHYESVGLFPQALRSDAGYRQY
                     SAREVNTLRFIRQSRDLGFSIEQIRELLGLWQNRRRPSRQVKALAQAHLQELEQKLLE
                     LHEMKTALEHLVHCCHGDDRPECPILESLADGSAIAPAAAKGGLRSGRPH"
     gene            complement(3073..5331)
                     /locus_tag="VNU48_05925"
     CDS             complement(3073..5331)
                     /locus_tag="VNU48_05925"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010399999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWI10848.1"
                     /translation="MNSALASLDKPLPVELTLQIEGMTCASCVARVEKALLKVPGVAS
                     ASVNLATERATVRAPTSLPWSALAAAVGKAGYAAVPTQATVPGKSLRLPAWWAAAAGA
                     ILTLPLLAPMLLQLVGIDWMLGAWVQLALATPIQFGLGARFYRAGWKALRAGSGNMDL
                     LVALGTSAAYGLSLYLMFRQAGHGMPHLYFEASAAVITLVLLGKWLEARAKRQTADAI
                     RALNALRPSIARIRRNGVEVDVPVEQIVLGDLVLVRPGDRIAVDGDIVEGRSHVDESL
                     ITGESLPVAKDAGGRVIGGSINADGALTVRTTAIGAETTLARIIRMVESAQAGKAPIQ
                     RIVDRVSAVFVPVVLGIALLTFFGWFGATGDWERALINAVAVLVIACPCALGLATPTA
                     IMVGTGAAARRGILIKDAQALELAHAVTTVAFDKTGTLTLGQPALVAVEPAGGRTADE
                     VLRLAAALQTRSAHPLASAVADAVRARQLAVPPAVEAKALPGRGLEATVLGEALALGS
                     SRLLRELGVDAGPLAAQAERLERDGRTISWLMRRQGGSAELIGLLAFGDTLKPSARQA
                     VERLRQLGIRTALLTGDNRGSAQAVARALGIDDVRAEVLPADKAAIVAQWRAAGQVVA
                     MIGDGINDAPALAAADVGIAMATGTDVAMEAAGITLMRGDPRLVADAIDVSRRTYSKI
                     KQGLFWAFAYNVLGIPLAALGLLNPVIAGAAMALSSVSVIGNALLLRRWRGAADVQAV
                     AAGAAQPRALSA"
BASE COUNT          774 a         1920 c         1951 g          779 t
ORIGIN      
        1 cggctgttcc aggtccacag gttgaagtcg agcatcgtgt tgacgcgcgg cgtggccgag
       61 ccggggtcga tgtcgtaggc cgccagcagc agcgcgaagt cgcgatcgac gcggtgctcg
      121 cgcgggtcct tcgggtggac gatgaacagg cccatcaggc ccatcgccat ctgcagcgac
      181 tcgtcggcat gcgggtggta catgtaggtg cccgaccgcg ccagcgcgaa ctcgtaaacg
      241 aaggtcttgc ccggcgcgat gccgggttgc gtcaggccgg tgacgccgtc catgcccgac
      301 ggcacgagca ggccgtgcca gtggatgctg gtcgcctcgg gcagcttgtt cgtcacgaag
      361 atgcgcacgc gctcgccttc gacggcctcg atcgtcgggc ccgggctcgc accgttgtag
      421 ccccacagat tcgcggccat gcccggcgcg agctcgcgca gcaccggttc ggcgacgagg
      481 tggaactcct tgacgccgtc cttcatgcgc cagggcagcg accagccgtt cagggtcacg
      541 accgggtggt agggccggcc gctggccggc gcgggcggcg gctgcatcgt cgtcgacacc
      601 tgcatcggcg cctcgggcag cgtggccgcg ccggcacgac tgacggcggc cgcgccgagc
      661 gcgctggccc cggcgcggcg caggaaagtt ctgcggttgt tcatcgtgat gccttcgagg
      721 gttcggtgtc gccgccggtc agcgcggctt gcagctggct ctgcgcaagc cagtggtcgc
      781 gcagcgcttc gacgcaggtg gtgacgctcg cgacctgctc gcgcgcgtcg gcgagcagct
      841 cgaagacgcc gatcagcatg ccgttgtagc gcagcaggtt ctcgtcggag atgcgcttgc
      901 gcagcggcag cacctcgtcg cggcagtgcc gcgcaagttc gtagctgctg cggtacgccg
      961 cgtacgactc gcgcacctgc gagcgcgcgt tgatcgcgac ctcggcggcg tgctggacgg
     1021 agcgcatgta gaccgcctcg gcgcgtgcgt ttcgcaccga gccgaagtcg aacagcggca
     1081 gttcgagctc gatctggtag ccgctgcgcc cggcttcgcc ggtctggctc ttgttcgcgt
     1141 agccgagctc cagcacgttg acgaagcgcg tcgcgttcgt caggcccagc tgctgcgcgg
     1201 tggcctgcgc cgagcgcttc gcgatcagca cgtcgaggcg ctgatcgagc gcggcctgct
     1261 ccgcgtcttg cgcttcggct ggcgcatcgg gcagatcggg cagtcgctcc ggcaacctga
     1321 acttcagctg gtcgccctgc aggccgagca gtcgtgtcag gcgttcgcgc tcgacaacgg
     1381 cctgatgctg cgcgcgcacc agctgcgcgc tcgcgtccgc ctggaacgcc tgctgacgca
     1441 gctgcgcgag gcggttgaag ttgccggcct ggagcatgcg acgcgccagt tcgccggacg
     1501 cgtcggcggc gtccttgacc tgaacggcat aggtcgccag ctcgccggcc gcgacggcgc
     1561 tgtagaacgc gcgccgcgcc tgcgcggcga cctgcacggc gtcgagcgcg gcctggtact
     1621 gggtctgctc gaagcgctgc tgctcgacct cgtgcgccgc cggaagcgtc aacagggcca
     1681 gcacgttgaa cagcatcgag cggtcgatct cggtgacgcc gccgccgcgc aggtgcgcga
     1741 acgccagcga tgggttcggc agccgcccga cgcgcaccag ctcggactcg gcgatgccga
     1801 gctcggcgaa gctcgcctgc aggccgcggt tgttcagcag cgcgagctcg acggcccggt
     1861 cggcgttcag cggttgcgcg agcagctcgg cgacgcgcgc ctgcgcggca tcgacgtcgg
     1921 ccggcgagcg ctgccacgat gccgtctggc cggtgcgttg ccgggtcagc tcggccacct
     1981 tgccgaagcc gccatcgggc gagaacgacg cgcagccgac ctgcgccagc gcgagcagcg
     2041 caacgctgca cgcgcgcgcc gccgcgctcg ggacgaatgg attcttcatg attcaaagcc
     2101 tctggaccgc cggcgggcat gaacccctcg gcgcgatgcc cgcgcgtgtg cgcgaacgcg
     2161 ttccggcctc ctgcggcgga ggccccacgg ggtcaggtgc ttcggggagg tcgctcctgc
     2221 ccatccgaga ggaagttggg cagcggcgcg aacagatcgg ggaacgacag cgtcgcgaag
     2281 ttcgtcgcca gcggcaaggc cgccgggctg ccgggcagcg tcgccatcga acagaagtcg
     2341 cagcacaggt tgcaggtcgg cgcgccaccg gcgtgatgct cgccgtgggc ggccgggtcg
     2401 tggcccgatg ccgccatgcc cgcgtggtgg gcgtgcatcg cgacggcacc gctctcgtga
     2461 tgcatgccgg tggacgcaca cggcatcgcc gccgcgaccg cgccccggat cggcagcagc
     2521 acggccagca gcaccagcag gcagaggcga agcgtcttca cgtcggaaag cgtagcacgg
     2581 cgcgggatgc gcgggcgccg gcgtcagtgc ggccggccgg agcgcaagcc gcccttggcc
     2641 gcagccggcg cgatcgcgct gccgtcggcc agcgactcca ggatcgggca ctcgggtcga
     2701 tcgtcgccat ggcagcaatg caccaggtgt tcgagcgccg tcttcatctc gtgcagttcg
     2761 agaagcttct gctcgagctc ctgcagatgc gcctgcgcca gcgccttcac ctggcggctc
     2821 gggcggcggc ggttctgcca cagccccagc agctcgcgga tctgctcgat cgagaagccc
     2881 aggtcgcgcg actggcggat gaagcgcagt gtgttgacct cgcgcgcgct gtactggcgg
     2941 tagcccgcgt cgctgcgcag cgcctgcggg aacaggccga cgctctcgta gtggcggatc
     3001 atcttcgcgg aaacgcccga ggccttcgcg gcctcgccga tgttcatcgg ttgcttctcc
     3061 atcgttcgct cctcaggcgc tcagggctcg gggctgcgcg gcgccggccg caaccgcttg
     3121 cacgtcggcc gcaccgcgcc agcgccgcag cagcaaggca ttgccgatga cgctgacgct
     3181 gctcagcgcc atcgccgcgc cggcgatgac cgggttcagc agccccagcg cggcgagcgg
     3241 aatgccgagc acgttgtacg cgaaggccca gaacaggccc tgcttgatct tcgaataggt
     3301 gcggcgcgac acgtcgatcg catcggcgac caggcgcggg tcaccgcgca tcagcgtgat
     3361 gccggccgcc tccatcgcga cgtcggtgcc ggtggccatc gcgatgccca cgtcggctgc
     3421 ggccagcgcc ggtgcgtcgt tgatgccgtc gccgatcatc gcgaccacct gaccggcggc
     3481 gcgccattgc gcgacgatcg cggccttgtc ggccggcagc acctcggcac gcacgtcgtc
     3541 gatgccgagc gcacgcgcga ccgcctgcgc actgccgcgg ttgtcgccgg tcagcagcgc
     3601 ggtgcggatg ccgagctggc gcagccgctc gaccgcctgg cgcgccgacg gcttgagcgt
     3661 gtcgccaaag gccagcagcc cgatcagctc ggcgctgcca ccctgcctgc gcatcagcca
     3721 cgagatcgtg cggccgtcgc gttcgagccg ttcggcttgt gcggccagcg gcccggcgtc
     3781 gacgccgagt tcgcgcagca gccggctgct gccgagcgcg agcgcctcgc cgaggacggt
     3841 cgcttccagc ccgcgaccgg gcaaggcctt cgcctcaacg gccggcggca ccgccaactg
     3901 ccgcgcgcga accgcgtccg ccaccgccga cgcgagcggg tgggcgctgc gcgtctgcag
     3961 cgccgccgcc aggcgcagca cttcgtccgc ggttcgaccg ccggccggct cgaccgccac
     4021 cagcgcgggc tggcccagcg tcagggtgcc ggtcttgtcg aaggccacgg tggtcaccgc
     4081 atgcgcgagt tccagcgcct gcgcatcctt gatcaggatg ccgcgccgcg cggcggcacc
     4141 ggtgccgacc atgatcgcgg tcggcgtggc cagcccgagc gcgcaggggc aggcgatcac
     4201 cagcaccgcc acggcgttga tcagcgcacg ctcccagtcg ccggtcgcgc cgaaccagcc
     4261 gaagaaggtg agcagcgcaa tgccgagcac caccgggacg aacaccgcgc tgacgcgatc
     4321 gacgatgcgc tggatcggcg ccttgcccgc ctgtgccgat tcgaccatgc ggatgatgcg
     4381 ggccagcgtc gtctcggcgc cgatcgcggt ggtcctgacg gtcagcgcgc cgtcggcgtt
     4441 gatcgagccg ccgatgacgc ggccgccggc gtccttggcg accggcaggc tctcgccggt
     4501 gatcagcgac tcgtcgacgt ggctgcggcc ttcgacgatg tcgccgtcaa ccgcgatgcg
     4561 atccccggga cgcaccagga cgagatcgcc gagcacgatc tgctcgaccg gcacgtcgac
     4621 ctcgacgccg ttgcgccgga tgcgcgcgat cgacggccgc agcgcattca gcgcgcggat
     4681 cgcatcggcc gtctgccgct tcgcgcgcgc ctcgagccat ttgccgagca gcacgagcgt
     4741 gatgaccgcg gccgaggcct cgaaatacag gtgcggcatg ccgtggccgg cctgccggaa
     4801 catcaggtac agggacaggc cgtacgcggc cgaggtgccg agcgcgacca gcaggtccat
     4861 gttgccggag ccggcgcgaa gcgccttcca gccggcacgg tagaagcgcg cgccgaggcc
     4921 gaactggatc ggcgtggcca gcgcgagctg aacccaggcg ccgagcatcc agtcgatgcc
     4981 aacgagttgc aacagcatcg gcgccagcag cggcagcgtc aggatcgcgc cggcagcggc
     5041 ggcccaccag gccggcagcc gcagactctt tccgggcacc gtagcctggg tcggaacggc
     5101 tgcatagccg gccttcccga ccgccgcggc aagcgcagac cacggcagcg aggtcggcgc
     5161 gcgcaccgtc gcgcgctcgg tcgcgaggtt caccgatgcc gacgccacgc cggggacctt
     5221 cagcagcgcc ttctcgacac gcgcgacgca cgacgcgcag gtcatgccct cgatctgcag
     5281 ggtcagctcg accggcagcg gcttgtcgag cgatgcgagc gcggagttca tggcggggtt
     5341 gtcgggtgca ttggggtcgg gttcagatga tcaaggttcc aacgatgtta atgtcaagcg
     5401 ggaacgggta gaaccaatgc ttcg
//
LOCUS       DATMJB010000266         4892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_370280, whole genome shotgun sequence.
ACCESSION   DATMJB010000266 DATMJB010000000
VERSION     DATMJB010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370280"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VNU48_05930"
     CDS             complement(<1..1146)
                     /locus_tag="VNU48_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWI10849.1"
                     /translation="MPGPPDAPAAWRTRLQCLSGLKRSLTAQISLSIAVISIALIAGS
                     GLLVNWLATRELREGNELIMFANLALLREDLAAANFDLTQTPQHLVKRMDLQLGHLHM
                     ALLDDQRRVIAASERFEIPIAALPKNAMAVDDLPSGIEHADVRALSKVLGPLTTVWTS
                     PDRRSFRLLFARIPVPAGSLAGPATRSLLVVFAYETTQTRQIVKNELQIFVATLLLGA
                     LAAGGLGVWIARRIVAGPKRLAAAANRISARPMSERLSVVHTPTELVDSTLAFNRMLD
                     RLEAAFKRLSEFSSDLAHDLRTPINNLLGEAQVTLAKPRSADEYRAVLESAVEDYERI
                     SRLIENMLFLARADDPRAAINRHWIDLRPLLARGRGYYELLAEERGVG"
     gene            complement(1130..1804)
                     /locus_tag="VNU48_05935"
     CDS             complement(1130..1804)
                     /locus_tag="VNU48_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HWI10850.1"
                     /translation="MRILIVEDEPKTAAYVSKGLSEHAYIVDVADNGIEGLHLASSGH
                     YDLILLDVMLPGADGWTVLEALRRQRQTPVLFLSARDEVADRVKGLELGADDYLVKPF
                     AFSELLARIRTLLRRGAAQIEQRVRLADLDVDRVRRRVSRGGERVELTAKEYALLDYF
                     ARHAGEVVSRTLIAEHVWDMNFDSDTNVVDVAVRRLRAKLDDPFPTKLIHTIRGVGYV
                     LDARPA"
     gene            complement(1937..3487)
                     /locus_tag="VNU48_05940"
     CDS             complement(1937..3487)
                     /locus_tag="VNU48_05940"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10851.1"
                     /translation="MRRQDIQLLALARQGDPAARCEAGRRYLLGVDGFPQHIATGLDY
                     LTHPNLRALPQAVRIIAESLALEDLLQLQQEGALAQAAAAGSASAQVKHGVWLCVRHG
                     RIEEGARWLDLAGAAGHEPALRAMAALRRPDADDPMVAFLNTLGASGELDGARVVTIA
                     AQQALAGRDLNRLAASLRTALALTSSSGTELAELVAAAVRLAEEQARPLVGLDPAAIE
                     AALDTRTSRGDRDAAYALGRALCGIACGVLAPQTLATGPNMRKGVALLLRAADAGRDE
                     AWLHLYRMHGDHSLSVANPQMARFCLEKAALGGQVEAQRKLGALLLRAADTLADSEQA
                     IEWLQQAARADDAHARALLQSLVLPTDGDDVQADAAIELVRRDDPWLAVRMQLARQFG
                     LTKLEALCVDPADGLRPWGLVVGKNPFISQIRLSAARAVPALSDAAMDVARRAASFFG
                     QASRDAQAFEGDLRRRSLRQRRTFERLGLDEAMFFANASSMTLEALRLGPKWAFRAKA
                     PLQLALAA"
     gene            complement(3708..4739)
                     /locus_tag="VNU48_05945"
     CDS             complement(3708..4739)
                     /locus_tag="VNU48_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HWI10852.1"
                     /translation="MKKFATLAVLAAISGGAYAQSVTLFGIIDEAARYVKNGDLKQKS
                     LVSGGINTSRLGVRGTEDLGGGLTAGFWLETGLNADSGTQSDPTRFWNRRSTVSLIGS
                     FGELRLGRDFTPTYTGYSDYDAFGDNGVAASGKFDSSLGTARDTGTRADNQIMYLTPA
                     NLGGFYGRAAVAAGEGTAGKKYIGARAGYAAGPLDVSAAYGQTTVAPLPLGGEDKFKT
                     ADIGASYDFGVVKATGYYTQSKFAAQKIASYSIGAIVPVGLGQIKAAYTHANASGTDA
                     LGVNVDANDANQFALGYIYNLSKRTAVYGTGAYIKNKGNATFAVASAPTLVPGGKSTG
                     LELGLRHSF"
BASE COUNT          707 a         1669 c         1775 g          741 t
ORIGIN      
        1 gccgacgccg cgctcttcgg ccagcagctc gtagtagccg cggccgcgcg ccagcagcgg
       61 ccgcaggtcg atccagtggc ggttgatcgc ggcgcgcggg tcgtcggcgc gcgccaggaa
      121 cagcatgttc tcgatcagcc gcgagatgcg ctcgtagtcc tcgacggcgg actcgagcac
      181 cgcgcggtat tcatcggcgc tgcgcggctt cgcgagcgtg acctgggctt cgccgagcag
      241 gttgttgatc ggcgtgcgca ggtcgtgcgc gaggtcggac gagaactccg acaggcgctt
      301 gaaggccgcc tccagccgat cgagcatgcg gttgaacgcg agcgtcgagt cgaccagttc
      361 ggtcggggtg tgcacgaccg acagccgctc gctcatcggc cgcgcgctga tccggttcgc
      421 cgcggccgcc aggcgcttcg gcccggcgac gatgcgccgc gcgatccaca cgcccagccc
      481 gcccgccgcg agcgcgccga gcagcagggt cgcgacgaag atctgcagct cgttcttgac
      541 gatctggcgg gtctgcgtcg tctcgtacgc aaacacgacc agcagcgatc gcgtggccgg
      601 gcccgcgagc gagccggccg gcaccggaat gcgcgcgaac agcagccgga agctgcggcg
      661 gtcgggcgag gtccagaccg tcgtcagcgg ccccagcacc ttgctcagcg cgcgcacgtc
      721 ggcgtgctcg atgccgctcg gcaggtcgtc gaccgccatc gcgttcttcg gcagcgccgc
      781 gatcggaatc tcgaaacgct cggacgcggc gatgacacgg cgctgatcgt cgagcagcgc
      841 catgtgcagg tgcccgagct gcaggtccat gcgcttgacc aggtgctgcg gcgtctgcgt
      901 caggtcgaag ttggcggccg ccaggtcctc gcgcagcagc gccaggttcg cgaacatgat
      961 cagctcgttg ccttcgcgca gctcgcgcgt cgcgagccag ttgacgagca ggccgctgcc
     1021 ggcgatcagc gcgatcgaga tcaccgcgat cgacaggctg atctgcgccg tcagcgagcg
     1081 cttcagcccg ctcaggcatt gcagccgcgt gcgccaggcc gcaggcgcgt caggcgggcc
     1141 gggcatcgag cacgtagccg acgccgcgaa tcgtgtggat cagcttggtc gggaacggat
     1201 cgtcgagctt ggcgcgcagg cggcgcaccg cgacgtccac gacattggtg tcgctgtcga
     1261 agttcatgtc ccacacgtgc tcggcgatca gcgtgcgtga gacgacctcg ccggcgtggc
     1321 gcgcgaagta gtcgagcagc gcgtactcct tggccgtcag ctcgacgcgc tcgccgccgc
     1381 ggctgacgcg gcggcgcacg cggtcgacgt cgaggtcggc caggcgcacc cgttgctcga
     1441 tctgcgccgc gccgcgccgc agcagcgtgc ggatgcgcgc gagcagctcg gagaacgcga
     1501 acggcttgac gagatagtcg tcggcgccga gctccaggcc cttgacgcgg tccgccacct
     1561 cgtcgcgcgc gctcaggaac agcaccggcg tctggcgctg gcggcgcagc gcctcgagca
     1621 cggtccagcc gtcggcgccg ggcagcatca cgtcgagcag gatcaggtcg tagtggccgg
     1681 agctcgcgag gtgcaggcct tcgatgccgt tgtcggcgac atcgacgatg taggcatgct
     1741 cgctcaggcc cttggacaca tacgcggccg tcttcggctc gtcttcgacg atcaggatgc
     1801 gcatggatac cacggccgag cggctgccgg ccaagcttgt tcacggaccg tcagtatccg
     1861 cgaagcgtcg atgcgacgac gcgcgccgcg acaatgccgg agcgcgcgtc ggggcgaatc
     1921 ggcgggcggg ctgttatcag gccgcgagcg cgagttgcag cggcgccttg gcgcgaaacg
     1981 cccatttcgg cccgagccgc agcgcctcga gcgtcatcga gctcgcgttg gcgaagaaca
     2041 tggcctcgtc cagaccgaga cgctcgaagg tgcgacgctg gcgcagcgaa cgccgccgca
     2101 ggtcgccctc gaaggcctgc gcgtcgcggc tggcctggcc gaagaacgat gcggcgcggc
     2161 gcgccacgtc catcgccgcg tcgctcagcg ccggcaccgc gcgcgccgcg gacaggcgga
     2221 tctgcgagat gaacgggttc ttgccgacca ccaaccccca gggccgcagg ccgtcggccg
     2281 gatcgacgca cagcgcctcg agcttggtca ggccgaactg ccgcgccagc tgcatccgca
     2341 ccgcgagcca cgggtcgtcg cggcgcacca gttcgatcgc cgcatcggcc tgcacgtcgt
     2401 caccgtccgt cggcagcacc agcgattgca gcagcgcacg ggcgtgcgcg tcgtcggcgc
     2461 gcgcggcctg ctgcagccac tcgatcgcct gctccgaatc ggccagcgtg tcggcggcgc
     2521 gcagcagcag cgcgccgagc ttgcgctgcg cctcgacctg cccgccgagc gccgctttct
     2581 cgaggcagaa acgcgccatc tgcggattcg cgaccgacag gctgtggtcg ccgtgcatcc
     2641 ggtacagatg cagccaggct tcgtcgcggc cggcgtcggc ggcgcgcagc agcagcgcca
     2701 cgcccttgcg catgttcggc ccggtcgcga gcgtctgcgg cgcgagcacg ccgcaggcga
     2761 tgccgcacag cgcgcggccg agcgcatagg cggcgtcgcg atcgcctcgg ctggtgcggg
     2821 tgtcgagcgc ggcctcgatc gccgcggggt ccaggccgac cagcggcctg gcctgctctt
     2881 cggccagacg cacggcggcg gccacgagtt cggccagttc ggtgccggac gacgatgtca
     2941 gcgcgagcgc ggtgcgcagg ctcgcggcga ggcgattcag gtcgcgcccg gcgagcgcct
     3001 gttgcgcggc gatcgtcacg acgcgcgcac cgtccagctc acccgaggca cccagcgtgt
     3061 tcaggaacgc gaccatcgga tcgtcggcat cgggccgacg cagggccgcc atcgcgcgca
     3121 gcgcgggctc gtggccggcc gcgccggcga ggtccagcca gcgcgcacct tcttcgatgc
     3181 gaccgtgccg cacgcacagc cacacgccgt gcttgacctg cgcgctcgcg ctgccggccg
     3241 cggcggcctg cgcgagtgcg ccctcctgct gcaactgaag caggtcttcg agcgcgaggc
     3301 tttcggcgat gatgcgcacg gcctgcggca gcgcgcgcag gttcgggtgc gtcaggtagt
     3361 ccaggccggt cgcgatgtgc tgcgggaagc cgtcgacccc gagcaggtag cggcgccccg
     3421 cctcgcagcg ggcggcgggg tcgccctgac gggcgagggc gagcagttgg atgtcttggc
     3481 gacgcattcg ggctccaggt gtggcgtgag cggcacgacc acgagccttt tgaggtcggg
     3541 gcatcgcacc gggtcaaggg aactgatgcg acgacgatag aaacgcggcg atgacccggc
     3601 gatggcacgt tgattacgat tccgtaatga acgctattga atatgacgcg gccgtgaaaa
     3661 gggccgcgtc cctcgcggaa cgcggccctc gatgcgagcg cgggtggtca gaagctgtgg
     3721 cgcaggccga gctccagccc tgtcgacttg ccgccgggca ccaaggtcgg cgccgaggcg
     3781 accgcgaagg tcgcattgcc cttgttcttg atgtaggcgc cggtgccgta gacggcggtg
     3841 cgcttggaca ggttgtagat gtagccgagc gcgaactggt tcgcgtcgtt cgcatcgaca
     3901 ttgacgccga gtgcatcggt gcccgacgca ttcgcgtggg tgtaggccgc cttgatctgg
     3961 ccgaggccga ccggcacgat cgcgccgatg ctgtagctgg cgatcttctg cgccgcgaac
     4021 ttcgactggg tgtagtagcc cgtcgccttg acgacaccga agtcgtagga cgcgccgatg
     4081 tcggcggtct tgaacttgtc ttcgccgccg agcggtagcg gtgccaccgt ggtctggcca
     4141 tacgcggccg acacgtcgag cgggcccgca gcgtagccgg cgcgtgcgcc gatgtacttc
     4201 ttgccggccg tgccttcgcc ggcggcgacc gcggcgcggc cgtagaagcc acccaggttg
     4261 gccggcgtca ggtacatgat ctggttgtcg gcgcgggtgc cggtgtcgcg tgcggtgccg
     4321 agtgacgagt cgaacttgcc cgacgcggcc acgccgttgt cgccgaacgc gtcgtagtcg
     4381 ctgtagccgg tgtacgtcgg cgtgaagtcg cggcccaggc gcagttcgcc gaagctgccg
     4441 atcaggctga cggtgctgcg gcggttccag aagcgggtcg ggtcggactg ggtaccgctg
     4501 tcggcattca ggccggtctc gagccagaag ccggccgtca ggccgccacc caggtcctcg
     4561 gtgccgcgca cgccgaggcg gctggtgttg atgccgccgc tgacgagcga cttctgcttc
     4621 aggtcgccgt tcttcacata gcgggcggct tcgtcgatga tgccgaacag cgtgaccgat
     4681 tgtgcgtagg cgccgccgga gatggcagca agcactgcca gggttgcgaa tttcttcatg
     4741 ctcttccttt gatttggtga ttcggacatg cccatgtccc ttgccacgag agcgaggaca
     4801 tgccgcagct taggaatcgc cgccggcgcc ggcacgcgtg tggcgcgaat cgccggggtc
     4861 aggctcgcgc gcccccctcg gaatgggaac ga
//
LOCUS       DATMJB010000267         2841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_855936, whole genome shotgun sequence.
ACCESSION   DATMJB010000267 DATMJB010000000
VERSION     DATMJB010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855936"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VNU48_05950"
     CDS             complement(<1..698)
                     /locus_tag="VNU48_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWI10853.1"
                     /translation="MRRTWLVFSQAVTVAVAVLFVVATLKPEWLQRGASVAPSIVSIT
                     SSPPLPLPVTLPGGAVTSYSAAAKRASPAVVSITASKAPARGPRSDDPVFQFFFGDRS
                     RAARGEPQIGLGSGVIVSAEGYLLTNNHVVDGADDIEVTLTDGRQARAKVVGTDPETD
                     VAVLKIELDRLPTISFGDAEKLQVGDVVLAIGNPFDVGQTVTSGIVSALGRNRLGINV
                     FENFIQTDAAINPG"
     gene            815..1852
                     /gene="pdxA"
                     /locus_tag="VNU48_05955"
     CDS             815..1852
                     /gene="pdxA"
                     /locus_tag="VNU48_05955"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HWI10854.1"
                     /translation="MSAEPTSSLPRRPLALTMGDACGIGPEIIARLFRGPESAGCVVL
                     GDVAVMRRATELTGGRLAVARVERAADALEAPPNCMPVLPVAGLPADLIAAGFGRVDA
                     RAGAAAAACIAHAVELARAGEIGAIVTAPIHKEALAAAGVPYPGHTEMLQALAAPGAT
                     PPPVRMMLANDELRTVLVTIHLSLRRAIEAVTFDAVLETIRIAHRSATRFGLGAPRIA
                     VAGLNPHAGEGGLFGDEERLIIGPAIAAARAEGIAASGPYAPDTVFMRARHAAGHPGE
                     FDIVVAMTHDHGLIPVKYLGVEHGVNVTLGLPFVRTSPDHGTAFDIAGSGRADVGSLA
                     AALRMARRLAA"
     gene            complement(1941..>2841)
                     /locus_tag="VNU48_05960"
     CDS             complement(1941..>2841)
                     /locus_tag="VNU48_05960"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HWI10855.1"
                     /translation="VWSQALVLAANRDGADSDRAQRYRRVARDLVMSVQPKGSPPLRK
                     KFLMQLPPLMKDLKEGLALIGWPEAAQRTFFARLLPAHAESLKAPALSELDHNMMVKQ
                     LENIFNVAVPASATLSPSDPVPEVDAEVLAQRFTPEEAQRVGLVSESAVDWSGTVDID
                     LGADFGPDSGNSMPGDSADVPLPGLDIDIALSSADPPEPTRGPALMDHVRLGFAYQMH
                     LKDEWQKVRLAHVSAARSFFVFTRGKKHQETISMTARMLARMCETNRFRAVESAYLIE
                     RATQRARRQLAALATPAHAATRV"
BASE COUNT          375 a         1010 c         1058 g          398 t
ORIGIN      
        1 ttgcccgggt tgatcgcggc gtcggtctgg atgaagttct cgaagacatt gatgcccagc
       61 cggttgcggc cgagcgcgct gacgatgccg gaggtcacgg tctggccgac atcgaacggg
      121 ttgccgatcg ccagcaccac gtcgccgacc tgcagcttct cggcgtcgcc gaagctgatc
      181 gtgggcaggc gatcgagttc gatcttgagt accgccacat cggtttccgg atcggtgccg
      241 accaccttgg cgcgggcctg gcggccgtcg gtcagcgtca cctcgatgtc gtcggcgccg
      301 tcgaccacgt ggttgttggt cagcaggtag ccctcggcgg agacgatcac gccggaaccg
      361 aggccgatct gcggctcgcc gcgcgccgcg cgtgaacggt cgccgaagaa gaactggaac
      421 accggatcgt cgctgcgcgg gccgcgcgcc ggcgccttgc tcgcggtgat gctgacgacc
      481 gcgggcgagg cgcgcttcgc ggccgcgctg taactcgtca cggcgccgcc ggggagcgtc
      541 accggcagcg gcagcggcgg gctggaggtg atcgagacga tgctcggcgc gacgctcgcg
      601 ccgcgctgca gccattcggg cttgagtgtc gcgacgacga acagcaccgc caccgcgacg
      661 gtgacggcct gcgaaaagac cagccaggtt ctgcgcatgg acacgcgggg aatcctcggg
      721 gaatgggccg ctcggtgcgg cgagacctgc tgcaattatc ggcgatggcc cgcggcctgg
      781 cgcccggcgc cgcgcgaatc gcgacaatgc ggccatgagc gccgaaccga cctcttcgct
      841 gccgcgccgg ccgctggccc tgacgatggg cgacgcctgc gggatcggcc cggagatcat
      901 cgcccggctg ttccgcggcc ccgaaagcgc cggctgcgtc gtgctcggcg acgtggcggt
      961 gatgcgccga gcgaccgaac tgaccggcgg gcggcttgcg gtcgcgcgcg tcgagcgcgc
     1021 cgccgacgcg ctggaggcgc cgccgaactg catgccggtg ctgccggtgg ccggcctgcc
     1081 ggccgacctg atcgcggccg gcttcgggcg cgtcgatgcg cgcgccggcg cggccgccgc
     1141 ggcctgcatc gcgcacgcgg tcgaactggc gcgcgccggc gagatcggcg cgatcgtgac
     1201 cgcgccgatc cacaaggagg cgctcgccgc ggccggtgtg ccttaccccg gccacaccga
     1261 gatgctgcag gcgctcgccg cgcccggcgc gaccccgccg ccggtgcgca tgatgctggc
     1321 caacgacgag ctgcgcacgg tgctggtgac gatccacctg tcactgcggc gcgcgatcga
     1381 ggcggtgacg ttcgacgcgg tgctcgagac gatccgcatc gcgcaccgca gcgcgacgcg
     1441 cttcgggctg ggcgcgccgc gcatcgcggt ggccgggctg aacccgcatg ccggcgaggg
     1501 cggtctgttc ggcgacgaag agcgcctgat catcggcccg gcgatcgccg cggcgcgcgc
     1561 cgaaggcatc gccgccagcg gcccctacgc gcccgacacg gtgttcatgc gcgcgcgcca
     1621 tgcggccggg cacccgggcg agttcgacat cgtcgtcgcg atgacgcacg accacgggct
     1681 gatcccggtc aagtacctgg gcgtcgagca cggcgtgaac gtcacgctcg gcctgccgtt
     1741 cgtgcgcacc agcccggacc acggcaccgc gttcgacatc gccggcagcg gccgcgccga
     1801 tgtcggcagc ctggccgcgg cgctgcgcat ggcgcggcgc ctggcggcct gacgctgcgg
     1861 ctcaggcggc gccgggccgc cccaagccgc ctgagcgccc ccttgggggg caggagcgaa
     1921 ccgacgtggg ggccgtcact tcagacccgc gtggcggcgt gcgcgggggt ggcgagcgcg
     1981 gcgagctgac gccgcgcgcg ctgcgtcgcg cgctcgatca ggtaggcgct ctcgaccgcg
     2041 cggaagcggt tcgtctcgca catgcgcgcg agcatgcgcg cggtcatcga gatcgtttcc
     2101 tggtgcttct tgccgcgcgt gaagacgaag aagctgcgcg ccgcgctgac gtgcgccagc
     2161 cgcaccttct gccactcgtc cttcaggtgc atctggtagg cgaagccgag ccggacgtgg
     2221 tccatcagcg ccgggccgcg cgtcggctcg ggcgggtcgg cgctggacag cgcgatgtcg
     2281 atgtccaggc ccggcagcgg cacgtcggcc gaatcgccgg gcatggagtt gcccgagtcg
     2341 gggccgaagt cggcgcccag gtcgatgtcc acggtgccgg accaatcgac cgcgctctcg
     2401 ctgaccaggc cgacacgctg cgcctcttcc ggcgtgaagc gctgcgcgag cacctcggcg
     2461 tcgacttcgg gcaccgggtc ggacggcgac agcgtcgcgc tggccggcac cgcgacgttg
     2521 aagatgttct ccagctgctt gaccatcatg ttgtggtcga gctcggacag cgccggcgcc
     2581 ttcagcgatt cggcatgcgc cggcagcagc cgggcgaaga acgtgcgctg cgccgcctcg
     2641 ggccagccga tcagcgccag gccttccttc aggtccttca tcagcggcgg cagctgcatc
     2701 aggaacttct tgcgcagcgg cggcgaaccc ttgggctgca cgctcatcac caggtcgcgg
     2761 gcgacgcgcc ggtagcgctg ggcgcggtcg gagtcggcgc cgtcgcggtt tgccgcgagc
     2821 accagtgcct ggctccagac c
//
LOCUS       DATMJB010000268         3262 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_596328, whole genome shotgun sequence.
ACCESSION   DATMJB010000268 DATMJB010000000
VERSION     DATMJB010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596328"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /gene="ndhC"
                     /locus_tag="VNU48_05965"
     CDS             <1..121
                     /gene="ndhC"
                     /locus_tag="VNU48_05965"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWI10856.1"
                     /translation="WAVTLREIGPTGFWAMMIFLGILVVGFAYEWKKGALDWE"
     gene            182..661
                     /locus_tag="VNU48_05970"
     CDS             182..661
                     /locus_tag="VNU48_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWI10857.1"
                     /translation="MAIDGVLKEGFVTTSVDALINWSKTGSLWPMTFGLACCAVEMMH
                     AGAARYDIDRFGMLFRPSPRQSDLMIVAGTLCNKMAPALRKVYDQMAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALLYGVLQLQAKIRRETTIARA"
     gene            658..1251
                     /locus_tag="VNU48_05975"
     CDS             658..1251
                     /locus_tag="VNU48_05975"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWI10858.1"
                     /translation="MKIDILEAALTAVLGDRVKTLVRDRGELTVTVSAANYIESALLL
                     RDHAELKFEQNIDLCGVDYSSYKDQAWEGPRFCVVSHLLSVSKNWRVRLKVFAPDDDV
                     PVVASLTDVWSSVNWFEREAFDLYGLIFEGHSDLRRILTDYGFIGHPFRKDFPISGHV
                     EMRYDDEQKRVIYQPVTIEPREITPRIIREDHYGGLH"
     gene            1259..2512
                     /locus_tag="VNU48_05980"
     CDS             1259..2512
                     /locus_tag="VNU48_05980"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWI10859.1"
                     /translation="MADIKNYTLNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAETRTFIQSLPYMDRLDYVSMMSNEHAYCLAIEKLLGVDVPVRAQYIRVMFD
                     EITRLLNHLMWLGAHGLDCGAMNIFIYCFREREDLFDMYEAASGARMHAAYYRPGGVY
                     RDLPDTMPQYRASKIRNAKAMSQLNENRQGSLLDFIDDFTKRFPKCVDDYETLLTDNR
                     IWKQRTVGIGVVSPERALNLGFTGAMLRGSGVLWDLRKHQPYEVYDKMDFDVPLGVNG
                     DTYDRYLVRVAEMRQANRIIQQCVAWLRANDGPVITSNYKVAPPPRVDMKSGMEDLIH
                     HFKLFTEGFHVPEGEAYAAVEHPKGEFGIYIVSDGANKPYRLKIRPPGFPHLASLDEL
                     SRGHMIADAVALIGTMDIVFGEIDR"
     gene            2509..2994
                     /gene="nuoE"
                     /locus_tag="VNU48_05985"
     CDS             2509..2994
                     /gene="nuoE"
                     /locus_tag="VNU48_05985"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HWI10860.1"
                     /translation="MISDSTRARFDKEVAKYPADQRQSAVMACLAIVQQEQGFVSGES
                     EKLVAEYLGMPPIAVHEVTTFYNMYNQRPLGRFKFNVCTNLPCQLRDGQQALDHLCHK
                     LGVDEGGTTADGLFTVQKSECLGACADAPVMLVNDRQMLSFMSHARLDELVETLKAAK
                     A"
     gene            2991..>3262
                     /locus_tag="VNU48_05990"
     CDS             2991..>3262
                     /locus_tag="VNU48_05990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849133.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HWI10861.1"
                     /translation="MKAQIDLSKFQATGAETCFHDRHIGAQIVDGLDGKNWRLKDYEA
                     RGGYEALRKILAQGEADGAALGMTPDQVIAEVKTSGLRGRGGAGFPT"
BASE COUNT          585 a         1064 c         1084 g          529 t
ORIGIN      
        1 gtgggcggtc acgctgcgcg agatcgggcc gacggggttc tgggcgatga tgatcttcct
       61 gggcattctg gtcgtcgggt tcgcctacga atggaaaaag ggcgcgctcg actgggagtg
      121 attccagctt gcgacgaatg aggcggtcgc cttcaccggc ggcgcaacgt tggcacacag
      181 catggcaatt gatggcgttc tgaaagaagg cttcgtcacg accagcgtcg atgcgctgat
      241 caactggtcg aagaccggat cgttgtggcc gatgaccttc ggcctcgcgt gctgcgcggt
      301 cgagatgatg cacgccggcg cggcgcgcta cgacatcgac cgcttcggca tgctgttccg
      361 ccccagcccg cggcaaagcg atctgatgat cgtcgccggc acgctgtgca acaagatggc
      421 gccggcgctg cgcaaggtct acgaccagat ggccgagccg cgctgggtgc tgagcatggg
      481 ctcgtgcgcg aacggtggcg gctactacca ctacagctac tcggtggtgc gcggctgcga
      541 ccgcatcgtg ccggtcgatg tctacgtgcc gggttgcccg ccgacggcag aggcactgct
      601 ctacggcgtg ctgcaactgc aggcgaagat tcgccgcgag acgacgatcg cgcgcgcatg
      661 aagatcgaca ttctcgaggc cgcgctgacg gctgttctcg gcgatcgcgt gaagacgctc
      721 gttcgcgacc gcggcgagct gaccgtgacg gtctcggccg cgaactacat cgaatcggcg
      781 ctgctgctgc gcgaccacgc cgagctgaag ttcgagcaga acatcgacct gtgcggcgtc
      841 gactactcga gctacaagga tcaggcgtgg gaagggccgc gcttctgcgt cgtctcgcac
      901 ctgctgtcgg tctcgaagaa ctggcgcgtg cgcctgaagg tgttcgcgcc ggacgacgat
      961 gtgccggtcg tcgcctcgct caccgatgtc tggagctcgg tcaactggtt cgagcgcgag
     1021 gccttcgacc tgtacggcct catcttcgag ggccacagcg acctgcgccg catcttgacc
     1081 gactacggct tcatcggcca cccgttccgc aaggacttcc cgatctcggg gcacgtcgag
     1141 atgcgctacg acgacgagca gaagcgcgtg atctaccagc cggtgacgat cgagccgcgc
     1201 gagatcacgc cgcggatcat tcgcgaagac cactacggcg ggctgcactg atcgcgtcat
     1261 ggccgacatc aagaactaca ccctgaactt cgggccccag cacccggccg cgcacggcgt
     1321 gctgcgcctc gtgctcgagc tcgacggcga ggtgatccag cgcgccgacc cgcacatcgg
     1381 gctgctgcac cgcgcgaccg agaagctcgc cgaaacgcgc accttcatcc agtcgctgcc
     1441 ctacatggac cgcctcgact acgtgtcgat gatgtccaac gaacacgcct actgcctggc
     1501 gatcgagaag ctgctcggcg tcgacgtgcc cgtccgcgcc cagtacatcc gcgtgatgtt
     1561 cgacgagatc acgcggctgc tgaaccacct gatgtggctg ggcgcgcacg gcctggactg
     1621 cggtgcgatg aacatcttca tctactgctt ccgcgagcgc gaggacctgt tcgacatgta
     1681 cgaggcggcg tcgggcgcgc gcatgcacgc ggcctactac cggccgggcg gcgtgtaccg
     1741 cgatctgccc gacacgatgc cgcagtaccg ggcctcgaag atccgcaacg cgaaggcgat
     1801 gtcgcagctc aacgagaacc gccagggctc gctgctcgac ttcatcgacg acttcacgaa
     1861 gcgcttcccg aagtgcgtcg acgattacga gacgctgctc accgacaacc gcatctggaa
     1921 gcagcgcacc gtcggcatcg gcgtcgtcag ccccgagcgc gcgttgaacc tgggcttcac
     1981 gggcgcgatg ctgcgcggct ccggcgtgct ctgggatctg cgcaagcacc agccctacga
     2041 ggtctacgac aagatggact tcgacgtgcc gctgggcgtg aacggcgaca cctacgaccg
     2101 ctacctcgtg cgcgtcgccg agatgcgcca ggcgaaccgc atcatccagc agtgcgtggc
     2161 ttggctgcgc gcgaacgacg ggccggtgat cacgagcaac tacaaggtcg cgccgccgcc
     2221 gcgtgtcgac atgaagtcgg ggatggaaga tttgatccac cacttcaagc tcttcaccga
     2281 aggcttccac gtgcccgagg gcgaggccta cgcggcggtc gagcacccga agggcgagtt
     2341 cggcatctat atcgtcagcg acggcgcgaa caagccgtac cggctgaaga tccgtccgcc
     2401 cggcttcccg cacctggcct cgctcgacga gctgtcgcgc ggccacatga ttgccgatgc
     2461 ggtcgcgctg atcggcacga tggacattgt tttcggagag atcgaccggt gatcagcgac
     2521 tccacgcgtg cgcgcttcga caaggaagtc gcgaagtacc cggccgacca gaggcagtcg
     2581 gcggtgatgg cctgcctggc gatcgtgcag caggagcaag gtttcgtctc gggcgagagc
     2641 gagaagctcg tcgccgagta cctcggcatg ccgccgatcg cggtgcacga ggtcacgacc
     2701 ttctacaaca tgtacaacca gcggccgctg ggccggttca agttcaacgt ctgcacgaac
     2761 ctgccgtgcc agttgcgcga cggccagcag gcgctcgacc acctgtgcca caagctcggc
     2821 gtcgacgagg gcggcacgac ggccgatggc ctgttcacgg tgcagaagag cgagtgcctg
     2881 ggcgcctgcg cggatgcgcc ggtgatgctg gtcaacgacc gccagatgct gagcttcatg
     2941 agccacgcgc ggctcgacga gctggttgaa accttgaagg cagcgaaggc atgaaggcgc
     3001 agatcgatct gtcgaagttc caggcgacgg gcgccgagac ctgctttcac gaccgccaca
     3061 ttggcgcgca gatcgtcgac ggcctggacg gcaagaactg gcgcctgaag gactacgagg
     3121 cgcgcggcgg ctacgaggcg ctgcgcaaga tcctcgcgca aggcgaggcc gacggcgcag
     3181 cgctcggcat gacgcccgat caggtcatcg ccgaggtcaa gacctcgggc ctgcgcggcc
     3241 gcggcggcgc gggcttcccg ac
//
LOCUS       DATMJB010000269         2335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_759779, whole genome shotgun sequence.
ACCESSION   DATMJB010000269 DATMJB010000000
VERSION     DATMJB010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759779"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VNU48_05995"
     CDS             <1..208
                     /locus_tag="VNU48_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010811530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HWI10862.1"
                     /translation="LSARNQLAGRVTRILPGAVNTEVVLELPGGGSVAAIVTNTSAMA
                     LGLAPGRAATAVFKASSVIVGVAA"
     gene            complement(245..1375)
                     /locus_tag="VNU48_06000"
     CDS             complement(245..1375)
                     /locus_tag="VNU48_06000"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWI10863.1"
                     /translation="MSRFDDPLHDREIGARDSTLDTTRIDDVRIGAVRPLITPALLQE
                     RVPLRDDSLPLVEKSRAAIADVLHGRDARLIVVVGPCSIHDHDQAIEYGHRLKAVADE
                     LQDDLLIVMRAYFEKPRTTVGWKGYINDPHLDGSFAINEGLERARRLLLELTTLGLPT
                     GTEFLDLLSPQFIADLIAWGAIGARTTESQSHRQLASGLSCPVGFKNGTDGSIRIAAD
                     AILAARAPHAFMGMTKMGMAAIFETRGNDDCHLILRGGRAPNFDAASVAGSCDTLRAA
                     GLREQVMIDVSHGNSSKQYRRQIEVADAVAAQIAGGERRIVGVMIESHLEEGRQDLVS
                     GQPLKHGVSITDACIGFAQTVPVLQALAGAVRDRRRVLLAND"
     gene            complement(1387..>2335)
                     /locus_tag="VNU48_06005"
     CDS             complement(1387..>2335)
                     /locus_tag="VNU48_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HWI10864.1"
                     /translation="MDAQTLAEEFLGDTIVSNIVAMGYAWQRGLVPVGLAAMQRAIEL
                     NGVAVESNLLSFALGRLAAGNPAALRDEASTKADDDTLEGLIARGINHLTGYQNAAWA
                     RRFEAAVRRAQQAEQACSGSDASLPLTRAVARSLMKLMSYKDEYEVARLYTDGGFQRQ
                     LADQFEGDVKLEFHMAPPLLARPKNGQPPKKIALGAWMLPAMKLLAKGKALRGSWFDL
                     FGHTEERRMERNLVTQFERCIDELAANLTAERLPSAIQIAALPQSMRGYGHVKIANVA
                     LTRAREAELLHRFMPERYAKPAPAVPPAGQFRGIAVVSR"
BASE COUNT          310 a          801 c          839 g          385 t
ORIGIN      
        1 gctgtcggcg cgcaaccagc tcgccggccg cgtgacacgc atcttgccgg gcgcggtgaa
       61 caccgaggtg gtactcgagc tgccgggcgg tggctcggtg gcggcgattg tcacgaacac
      121 gagcgcgatg gcgctggggc tcgcgccggg gcgtgcggcc accgcggtct tcaaggcgtc
      181 gagcgtgatc gtcggcgtgg ccgcctgaag agcggtcgcc gctccccgac tcggtggccg
      241 gtgatcagtc gttcgccagc aggacgcgcc ggcgatcgcg gaccgcgccg gccagcgcct
      301 gcaacaccgg caccgtctgc gcgaagccga tgcaggcgtc ggtgatcgac acgccgtgct
      361 tcagcggctg gccggagacc agatcctgtc ggccctcctc gaggtggctc tcgatcatca
      421 cgccgacgat gcggcgctcg ccaccggcga tctgcgccgc gactgcgtcg gccacttcga
      481 tctggcgccg gtactgcttg ctgctgttgc cgtgcgacac gtcgatcatc acctgctcgc
      541 gcaggccggc ggcgcgcagc gtgtcgcagc tcccggccac gctcgcggca tcgaagttcg
      601 gcgcgcggcc gccgcgcagg atcaggtggc agtcgtcgtt gccgcgggtc tcgaagatcg
      661 ccgccatgcc catcttcgtc atgcccatga aggcgtgcgg cgcgcgcgcc gcgaggatcg
      721 cgtcggcggc tattctgatg ctgccgtcgg tgccgttctt gaagccgacc gggcagctca
      781 ggcccgaggc gagctgacgg tggctctggc tctcggtggt gcgcgcgccg atcgcgcccc
      841 aggcgatgag gtcggcgatg aactgcgggc tcagcaggtc gaggaactcg gtgccggtgg
      901 gcagcccgag ggtggtgagc tccagcagca ggcggcgcgc gcgctccagc ccttcgttga
      961 tggcgaagct gccgtccagg tgcgggtcgt tgatgtagcc cttccagccc accgtcgtgc
     1021 gcggcttctc gaagtacgcg cgcatcacaa tcagcaggtc gtcctgcaac tcgtcggcga
     1081 cggccttcag ccgatggccg tactcgatcg cctggtcgtg gtcgtggatc gagcacgggc
     1141 cgacgacgac gatgagccgc gcgtcgcgcc cgtgcagcac gtcggcgatc gcggcgcggc
     1201 tcttctcgac cagcggcaag ctgtcgtcgc gcagcggcac gcgctcctgc agcagcgccg
     1261 gcgtgatcag cgggcgcacc gcgccgatgc gcacgtcgtc gatgcgggtc gtgtccagcg
     1321 tgctgtcgcg cgcgccgatc tcgcggtcgt gcaaggggtc atcgaatctg ctcatgggcg
     1381 cggagcttat cgtgacacga cggcgatgcc gcggaactgg cccgccggcg gcactgcagg
     1441 cgcaggcttt gcgtagcgct ccggcatgaa gcggtgcagc agctcggcct cgcgggcgcg
     1501 ggtcagcgcg acgttggcga tcttcacgtg gccgtaaccg cgcatgctct gcggcagcgc
     1561 cgcgatctgg atcgcgctcg gcaggcgctc ggcggtgaga ttcgccgcga gctcgtcgat
     1621 gcagcgctcg aattgcgtca ccaggttgcg ctccatgcgc cgctcttcgg tgtggccgaa
     1681 gaggtcgaac cagctgccgc gcaaggcctt gcccttggcg agcagcttca tggccggcag
     1741 catccacgcg ccaagcgcga tcttcttcgg cggctggccg ttcttcggcc gcgcgagcag
     1801 cggcggagcc atgtggaatt cgagcttcac atcgccttcg aactggtcgg ccagctggcg
     1861 ctggaagccg ccgtcggtgt acaggcgcgc gacctcgtat tcgtccttgt agctcatcag
     1921 cttcatcagg ctgcgggcga cggcgcgcgt cagcggcagc gaagcgtcgc tgccagaaca
     1981 tgcctgctcg gcctgctgcg cgcgacgcac ggcagcttcg aagcggcgcg cccacgcggc
     2041 gttctggtag ccggtgaggt gattgatgcc acgcgcgatc aggccttcga gcgtgtcgtc
     2101 gtcggccttc gtgctcgctt cgtcgcgcag cgccgccggg ttgccggccg ccaggcggcc
     2161 gagcgcgaac gacagcaggt tgctctcgac cgcgacgccg ttcagctcga tcgcccgttg
     2221 catcgccgcc aggcccaccg gcaccaagcc gcgctgccag gcgtagccca tggcgacgat
     2281 gttggagacg atggtgtcgc cgaggaactc ctcggccagg gtctgcgcat ccatg
//
LOCUS       DATMJB010000270         5895 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_122367, whole genome shotgun sequence.
ACCESSION   DATMJB010000270 DATMJB010000000
VERSION     DATMJB010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122367"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..1368
                     /gene="egtB"
                     /locus_tag="VNU48_06010"
     CDS             79..1368
                     /gene="egtB"
                     /locus_tag="VNU48_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HWI10865.1"
                     /translation="MKTAPWPMPAVAQPGELAAAYAAIRQTTLDLIAPLSAEDCQVQS
                     MPDASPAKWHLAHVTWFFETFVLERHEADFRPFDASFRVLFNSYYQGVGEMHPRAQRG
                     LITRPTLAEVKQYRAAVDERMQRLLGARGDDAALAQLVTLGLHHEQQHQELLLTDIKH
                     ALSFNPARTAYVRRWPMTSVRAQPLRWFGQTGGLIEQGFDTALDGAFCFDNETPRHGV
                     YLAPFELASRPVNNGEYLAFIDDAGYRRPELWLSMGWDWVRAGQRSAPLYWRELDGRW
                     MCHTLHGLVEIDTRTPVCHLSYFEADAFARWAEARLPTEFEWEHAARQLRAATRPNLA
                     DRGAFHPLPAEGDAGDEPVQMFGDVWEWTLSSYSAYPGYRPLPGAVGEYNGKFMSNQY
                     VLRGGSCATPAGHVRASYRNFFPTDAQWQFSGLRLAR"
     gene            complement(1453..2157)
                     /locus_tag="VNU48_06015"
     CDS             complement(1453..2157)
                     /locus_tag="VNU48_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF599 domain-containing protein"
                     /protein_id="HWI10866.1"
                     /translation="MDVMFKVLALLPWLDWAALAVFFVGWGAYAHFSRRGSGSKRSVL
                     AATNAVRRQWMLQTTYREVRVIDGVVIQNLSTSPSFFASTTILIIGGLLAVLAGDKAN
                     DLVRDLPFAQRTSALIFDLKVILLLGIFVYAFFRFTWSMRQYTFGALLIAAAPEAGRF
                     IAETLSREAFADKAGRVVGLAAETFNDGLRGYYFAFAAIGWFFSPLVFMLATLGVIYI
                     LYQREFRSEVLSVLTS"
     gene            complement(2210..2620)
                     /locus_tag="VNU48_06020"
     CDS             complement(2210..2620)
                     /locus_tag="VNU48_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWI10867.1"
                     /translation="MFNSLQNPLALLGRILLALIFISSGFSKIAGFEGVVGYIASKGV
                     PMASVVAALTILIELGGGLAIAFGFMTRWAALALAVFSLLAGLIFHAYWAVPADQVMN
                     MQINFWKNVSIAGGFLALAAFGPGAISVDARRAH"
     gene            complement(2659..3378)
                     /locus_tag="VNU48_06025"
     CDS             complement(2659..3378)
                     /locus_tag="VNU48_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWI10868.1"
                     /translation="MLTIRKSSDRGHADHGWLESFHSFSFADYHDPQHMGFGNLRVIN
                     EDRVAPGTGFGTHGHRDMEIVSYVLSGELAHKDSMGNGEAGAAVSGVIRPGDVQRMSA
                     GRGVLHSEFNHAPNDTTHFLQIWIEPSVRGIEPGYEQKHFDAASKRGTLRLVASPAGA
                     DGSVTIHADASIYSGLFNGDPPLKCALDPKRRAYVHVVRGSVTVNGQPLQGGDALRLE
                     RESELTIADGVGAEVLVFDLH"
     gene            3492..4529
                     /locus_tag="VNU48_06030"
     CDS             3492..4529
                     /locus_tag="VNU48_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWI10869.1"
                     /translation="MPDSRNVLTPEALTMMDVIARTGSFAAAARELGKVPSSLTYSVR
                     QLEDALDVLLFDRRSRQARLTAAGEELLNEGRRLLDQMDAVANRVKRVATGWETQLSI
                     AVDGVISRLTMFELCEAFYALNPQCPTAHGSGAELPATLGNPGTRLRLRTEVMTGTWE
                     PLITGQADLAIGVGTDRSVPTGVQMKELGTVDFVFAVAPHHPLAAGSVPISDAELLRH
                     RAVAVADSAQRLTPLTVNLLPGQDVLTVSSMQLKIEALLRCMGCGFVPLPMVREHIAA
                     GRLVAKPVQRVGRPARLGYAWRTPVANAPGAARKPQLGLALRWWLEQLDSPTTRSALI
                     ERQGQASTVGM"
     gene            4550..5455
                     /gene="gluQRS"
                     /locus_tag="VNU48_06035"
     CDS             4550..5455
                     /gene="gluQRS"
                     /locus_tag="VNU48_06035"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HWI10870.1"
                     /translation="MSTAAYRGRFAPSPTGLLHAGSLAAALASWLDARAHGGEWLLRI
                     EDVDAPRCVPGADAAIVAQLAACGLVSDEPPQWQSVRGAHYEAAIARLVAEGWVYPCG
                     CTRADIERALAQQGRVRARHGDLVYPGTCRSGLQGKPARALRLRATPSIDAPAAPLDI
                     AWHDRRLGAQHQCVSDAVGDFVLKRADGLWAYQLAVVVDDAAQGITHVVRGEDLADNT
                     PRQIHLQRVLGLATPAYLHTPLALGADGAKLSKQNGAVPVDTRNPLAVLREAARLLDV
                     QAEGTSVPELLASATAHWAARWRAV"
     gene            5547..5894
                     /locus_tag="VNU48_06040"
     CDS             5547..5894
                     /locus_tag="VNU48_06040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWI10871.1"
                     /translation="MVTTESGLQYEDTTVGSGATARAGQHVSVHYTGWLYNNGAKGAK
                     FDSSKDRNDAFSFDLGQRQVIAGWDEGVQGMKIGGTRKLTIPPQLGYGARGAGGVIPP
                     NATLMFEVELLGV"
BASE COUNT          935 a         2059 c         2050 g          851 t
ORIGIN      
        1 ctggcgccgg cccgaccggt gtaagttcgg gtcgctcttg caacgattct ccgtggccac
       61 acccacggag cgcatgccat gaagactgcc ccgtggccca tgcccgccgt cgcgcagccg
      121 ggcgaactcg cggccgccta cgcggcgatc cgccagacca cgctggacct gatcgcgccg
      181 ctgtcggccg aggattgcca ggtccagtcg atgcccgatg cgagcccggc gaagtggcat
      241 ctcgcgcacg tcacctggtt cttcgagacc ttcgtgctgg agcgccatga ggccgacttc
      301 cggccgttcg acgcgagctt ccgggtgctg ttcaacagct actaccaggg tgtgggcgag
      361 atgcacccgc gcgcccagcg cgggctcatc acgcgcccga cgctggccga ggtgaagcag
      421 taccgcgccg ccgtcgacga gcgcatgcag cggctgctcg gcgcgcgcgg cgacgacgcg
      481 gcgctggctc agctggtgac gctcgggctg caccatgagc agcagcatca ggagctgctg
      541 ctcaccgaca tcaagcacgc gctgagcttc aaccccgcgc gcaccgccta cgtcaggcgc
      601 tggccgatga ccagcgtgcg cgcgcagccg ctgcgctggt tcggccagac cggcgggctg
      661 atcgagcagg gtttcgacac cgcgctcgac ggcgcgttct gcttcgacaa cgagacgccg
      721 cgccacggcg tctacctggc gccgttcgaa ctcgcgtcgc gcccggtgaa caacggcgag
      781 tacctcgcgt tcatagacga cgccggctac cgccgccccg agctctggct gtcgatgggc
      841 tgggactggg tgcgcgccgg tcagcgcagc gcgccgctgt actggcgcga actcgatggg
      901 cgctggatgt gccacacgct gcacggcctg gtcgagatcg acacgcgcac gcccgtgtgt
      961 cacctcagct acttcgaggc cgacgcgttc gcgcgctggg ccgaggcgcg gctgcccacc
     1021 gaattcgaat gggagcacgc ggcgcggcag ctgcgcgccg cgacgcggcc gaacctggcc
     1081 gaccgcggcg ccttccaccc gctgccggcc gagggcgacg ccggtgacga gccggtgcag
     1141 atgttcggcg atgtctggga gtggacgctg tcgagctaca gcgcctaccc gggctaccgc
     1201 ccgctgccgg gcgcggtggg cgaatacaac ggcaagttca tgagcaacca gtacgtgctg
     1261 cgcggcggct cgtgcgcaac gcctgccggt cacgtgcgcg cgagctaccg caacttcttc
     1321 ccgaccgacg cgcagtggca gttcagcggt ctgcggctgg cgcgatgaca gcccctcgac
     1381 cgcgggtacg cggtcgatcc cccgcgggga tgccgcccgg cttggggcgg cccggcgctc
     1441 gggcggctcg acctacgagg tcagcacgct cagtacctcg gagcggaact cgcgctggta
     1501 caggatgtag atcacgccca gcgtcgcgag catgaagacg agcggcgaga agaaccagcc
     1561 gatcgcggcg aatgcgaagt agtagccgcg cagcccgtcg ttgaaggtct cggcggccag
     1621 gccgacgacg cgcccggcct tgtcggcgaa ggcctcgcgc gacagtgtct cggcgatgaa
     1681 gcgcccggcc tcgggggcgg ccgcgattag cagcgcgccg aacgtgtact ggcgcatcga
     1741 ccaggtgaag cggaagaacg cgtagacgaa gatgccgagc agcaggatca ccttcagatc
     1801 gaagatcagc gccgaggtgc gctgcgcgaa cggcaggtcg cgcaccagat cgttggcctt
     1861 gtcgcccgcg agcactgcca gcaggccgcc gatgatcagg atcgtcgtcg acgcgaagaa
     1921 cgacgggctg gtcgacaggt tctggatcac gacgccgtcg atcacccgga cctcgcggta
     1981 ggtcgtctgc agcatccact gcctgcgcac ggcgttggtc gccgcgagca ccgagcgctt
     2041 cgagccggag ccgcgccgcg agaagtgggc gtacgcgccc cagccgacga agaacaccgc
     2101 cagcgccgcc cagtcgagcc agggcagcag cgccaagacc ttgaacatca cgtccatgga
     2161 cggaaatgta gcaaggccgg ggccgcgcgc gccggccccg acaaggggtt cagtgcgcgc
     2221 gcctcgcgtc gacgctgatc gcgcccgggc cgaacgcggc gagcgccagg aagccaccgg
     2281 caatcgagac gttcttccag aagttgatct gcatgttcat cacctggtcg gccggcaccg
     2341 cccagtaggc gtggaagatc aggcccgcga gcaggctgaa gaccgccagc gccagcgctg
     2401 cccagcgcgt catgaaaccg aacgcgatcg cgagaccacc gccgagttcg atgaggatgg
     2461 tcagtgcggc caccaccgag gccattggca cacccttcga ggcgatgtag ccgaccacgc
     2521 cctcgaagcc ggcgatcttg ctgaatccgg agctgatgaa gatgagcgcc agcaggatgc
     2581 gaccgagcag cgcgagcggg ttctgaagac tgttgaacat gatgtttcct tgtgttgaag
     2641 gtcgatgaaa ttgcggaatc agtggagatc gaagaccagc acttcggcac cgacaccgtc
     2701 ggcgatcgtc agttcgctct cgcgttcgag gcgcagtgcg tcgccgccct gcaagggctg
     2761 gccattcacg gtgacgctgc cgcgcacgac gtgcacgtag gccctgcgct tcgggtcgag
     2821 cgcgcacttc agcggcggat caccattaaa caggccgctg tagatcgacg cgtcggcgtg
     2881 gatcgtgacc gagccgtcgg cgccggccgg cgacgcgacc aggcgcagcg tgccgcgctt
     2941 ggacgccgca tcgaaatgct tctgctcgta gcccggctcg atgccgcgca cgctgggctc
     3001 gatccagatc tgcaggaagt gcgtggtgtc gttcggcgcg tggttgaact cgctgtgcag
     3061 cacgccccgg ccggcgctca tgcgctgcac gtcgccgggc cggatcacgc cgctgaccgc
     3121 cgcgccggct tctccgttgc ccatgctgtc cttgtgcgcg agctcgcccg acagcacgta
     3181 gctgacgatc tccatgtccc ggtggccgtg cgtgccgaaa ccggtgccgg gcgcgacgcg
     3241 gtcttcgttg atgacgcgca ggttgccgaa acccatgtgc tgcgggtcgt ggtagtcggc
     3301 gaacgagaag ctgtgaaaac tctcgagcca gccgtggtct gcatggccgc ggtcggagga
     3361 tttgcgaatg gtgagcatgg cgacgttcct ttccttgcga tgaatgtagg ttccggcacg
     3421 cgactcggcg atcggtgcac gttgaagtga tcgttcaaat aagatgaacg acccttttaa
     3481 gctcagtccc catgcccgat agccgcaatg tcctgacccc cgaggccctc acgatgatgg
     3541 atgtcatcgc ccgcaccggc agcttcgccg ccgcggcgcg cgagctcggc aaggtgccgt
     3601 cgtcgctgac ctacagcgtg cgccagctcg aggacgcgct cgacgtgctg ctgttcgatc
     3661 gacgctcgcg ccaggcgcgg ctcaccgcgg ccggcgagga actgctgaac gagggccggc
     3721 gcctgctcga tcagatggac gcggtcgcga accgcgtcaa gcgcgtcgcc accggctggg
     3781 agacgcagct cagcatcgcg gtcgacggtg tgatctcgcg cctgaccatg ttcgaactgt
     3841 gcgaggcgtt ctacgcgctc aacccgcagt gcccgacggc ccacggcagc ggcgccgagc
     3901 tgccggccac gctcggcaac ccgggcacgc ggctgcgcct gcgcaccgaa gtgatgaccg
     3961 gcacctggga gcccctgatc accggccagg ccgatctggc gatcggcgtc ggcaccgacc
     4021 gcagcgtgcc caccggcgtg cagatgaagg agctcggcac ggtggacttc gtgttcgcgg
     4081 tcgcgccgca ccacccgctg gccgcgggca gcgtgccgat cagcgatgcc gagttgctgc
     4141 ggcaccgcgc ggtcgcggtc gccgattcgg cgcagcgcct gacgccgttg accgtgaacc
     4201 tgctgccggg gcaggacgtg ctgaccgtca gcagcatgca gctcaagatc gaagcgctgc
     4261 tgcgctgcat gggctgcggc ttcgtgccgc tgccgatggt gcgcgagcac atcgcggccg
     4321 gtcggctcgt cgccaagccg gtgcaaagag tcggccgacc ggcgcggctc ggctatgcct
     4381 ggcgcacgcc ggtcgccaac gcgccgggcg cggcgcgcaa gccgcagctc ggcctggcac
     4441 tgcgctggtg gctggagcag ctcgacagcc cgaccacgcg cagcgcgctg atcgagcgcc
     4501 agggccaggc atcgaccgtc ggcatgtgat cggagcgggt cgcatcgcga tgtcgacagc
     4561 agcctatcgc ggccggttcg cgccctcgcc gaccgggctg ctgcacgccg gctcgctggc
     4621 cgcggcgctc gcctcgtggc tggatgcgcg cgcccacggc ggcgagtggc tgctgcgcat
     4681 cgaggatgtc gacgcgccgc gctgcgtgcc cggtgccgac gcggcaatcg tcgcgcagct
     4741 cgcggcctgc gggctggtgt cggatgagcc gccgcaatgg caatcggtac gcggcgcgca
     4801 ctatgaagcc gcgatcgcgc ggctggtcgc cgagggttgg gtctacccct gcggctgcac
     4861 gcgtgccgac atcgaacgcg cgctggcgca gcaggggcgc gtgcgcgcgc gccacggcga
     4921 tctcgtctac cccggcacct gccgcagcgg attgcagggc aaaccggcgc gtgcactgcg
     4981 gctgcgggcc acgccctcga tcgacgcgcc ggccgcgccg ctggacatcg cgtggcacga
     5041 ccggcgcctc ggcgcgcagc accagtgcgt cagcgacgcg gtcggcgact tcgtgctcaa
     5101 gcgcgccgac ggcctgtggg cctaccagct cgccgtcgtc gtcgacgacg cggcgcaggg
     5161 catcacgcac gtcgtgcgtg gcgaggatct ggccgacaac acgccgcgcc agatccacct
     5221 gcagcgcgtg ctcgggctgg cgacgccggc gtatctgcac acgccgcttg cgctcggcgc
     5281 cgacggcgcg aagctgtcga agcagaacgg cgccgtgccg gtcgacaccc gcaaccccct
     5341 ggctgttttg cgcgaggctg cgcgcctgct cgacgtacag gccgagggca ccagcgtgcc
     5401 cgagctgctg gccagcgcga cggcacactg ggccgcgcgc tggcgcgccg tctgaccgca
     5461 gcaccagacc ccggcctgcg ccgctcgcgt cggcggctgg catgatcgcg gttcacgttc
     5521 gtttcaacca cccacgaagg ctcgtcatgg tcaccaccga atccggtctg caatacgaag
     5581 acaccaccgt cggcagcggc gccaccgcgc gcgccggcca gcacgtgtcg gtccactaca
     5641 ccggctggct ctacaacaac ggcgcgaagg gcgcgaagtt cgactccagc aaggaccgca
     5701 acgatgcctt cagcttcgac ctcggccagc gccaagtcat cgccggctgg gacgaaggcg
     5761 tgcaaggcat gaagatcggc ggcacgcgca agctcaccat cccgccccag ctcggctacg
     5821 gcgcacgcgg cgccggcggc gtgattccac cgaacgcgac gctgatgttc gaggtcgagc
     5881 tgctgggcgt ctgaa
//
LOCUS       DATMJB010000271         2187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_877795, whole genome shotgun sequence.
ACCESSION   DATMJB010000271 DATMJB010000000
VERSION     DATMJB010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877795"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VNU48_06045"
     CDS             <1..448
                     /locus_tag="VNU48_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWI10872.1"
                     /translation="IAWLDRLPTRQGVDIAVDAQTLEDPYWASQRKGVRVLPRPGRVA
                     EMDFPVVLTSEIDGTVYLIDKAARRGIGDVLIELLDNERQVVSSVKSGSDGYYIVPAV
                     VQGRYFLRVSPEQLKQFELIDPGERAITIAPDGKFINGVEFLLHKK"
     gene            585..737
                     /locus_tag="VNU48_06050"
     CDS             585..737
                     /locus_tag="VNU48_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10873.1"
                     /translation="MTDIGLVDGDVYRVGLMLMAVRSLGVNRQVDASRPGEVEPHDVE
                     LLRLKP"
     gene            734..1450
                     /locus_tag="VNU48_06055"
     CDS             734..1450
                     /locus_tag="VNU48_06055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWI10874.1"
                     /translation="MIEHERAHDAERVRSAEALAQANARLRQLSAHLVDAQERERTHI
                     ARELHDGLGQLLTGIVIQLHAALRSESSAQAERCRGDALDLAQEAIRQVKSMSFQLRP
                     AQLELLGFVAAVKATLDRQRDATGLQSFVRLRGQAPQQAVLSNAVALRILQEALNNVL
                     RHAAASRLVVRLRFNGDDHFVMTIGDDGCGFNVRAVLAGGMSENNVGLHGMLERAELM
                     GGRLRFRSSIGRGSVLRLSV"
     gene            1447..2124
                     /locus_tag="VNU48_06060"
     CDS             1447..2124
                     /locus_tag="VNU48_06060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI10875.1"
                     /translation="MNKGTTSTGPNPGGTVRIVLADDHDLVRSGLKLLLEMVAGVAVV
                     GQARNGEELLAEVGRHAPDLVVTDLSMPGMDGLTALAALRAMPQPPRVLVVSMHDSPD
                     FIRRALQLGAAGYVMKESSPLELEHAVRSVMAGVPYYSAQVSQRLLLARERSPEEMLT
                     ERQLEILVLIALGQAAKEIAFKLSLSPKTVDVHRARIMERLGINDLPGLTLYCVRHGL
                     VDPVRHA"
BASE COUNT          374 a          699 c          752 g          362 t
ORIGIN      
        1 catcgcctgg ctcgatcgct tgcccacgcg tcaaggcgtc gatatcgcag tcgatgcgca
       61 gacgctggaa gacccttatt gggcatcgca acgcaagggc gtgcgcgtgc tgccgcgtcc
      121 gggccgcgtc gccgaaatgg acttcccggt cgtcctgacc agcgaaatcg acggcacggt
      181 ctatttgatc gacaaggccg cacgacgcgg gatcggcgac gtgctgatcg aactgctcga
      241 caacgagcgc caggtcgtgt cctccgtcaa gagcggatcg gacggctact acatcgtccc
      301 ggccgtggtc cagggtcgct acttcctgcg cgtatcgccc gagcaattga agcagttcga
      361 gctgatcgat cccggcgagc gcgcaatcac cattgcgccc gatggcaagt tcatcaacgg
      421 cgttgaattc ctgttgcaca agaaatgatc cgccccgacg gccgaggcgg tcgggatgtg
      481 caaagcgaaa tgcccgagcg ttccaattcg gctgccatga cacgccgcgt gggatcatcg
      541 ggcatgggcg cggcgccgca ggcaaccttg acgacggaga agagatgacc gatatcgggc
      601 tggtcgacgg cgacgtctat cgcgtggggc tcatgctgat ggccgtgcgt tcgctcggcg
      661 tgaatcgtca ggtcgatgcc tcccggccgg gagaggtcga gccgcatgat gtcgaactcc
      721 ttcgactgaa gccatgatcg agcacgagcg agcgcacgac gccgaacggg tgcgcagcgc
      781 cgaggcgctg gcgcaagcca atgctcggct gcgccaactg tcggcgcacc tggtcgacgc
      841 gcaggagcgc gagcgcacgc acatcgcacg cgaattgcac gatgggctcg gccagttgct
      901 gaccggcatc gtgatacagc tccacgcggc gttgcgaagc gagtcgtcgg cgcaggccga
      961 gcggtgtcgc ggcgacgcac ttgatctggc gcaggaggca atccggcagg tcaaatcgat
     1021 gtcgttccag ctgcgcccgg ctcagctgga gttgctcggc ttcgtcgctg ccgtcaaggc
     1081 gacgctcgac cgccagcgcg acgccaccgg acttcagagt ttcgtgcgcc tgcgcggcca
     1141 ggcgccgcag caggcggtgc tgtccaacgc ggtagcgctg cgtatcctgc aggaggcctt
     1201 gaacaacgtg ctgcgccacg cggcggcgag ccggctggtc gtgcggctgc gcttcaacgg
     1261 agatgaccat ttcgtgatga cgatcggtga cgacggctgc ggcttcaacg tgcgtgcggt
     1321 gctcgcgggc ggtatgagcg agaacaacgt cggcctgcac ggcatgctcg aacgtgccga
     1381 gctgatgggc gggcgactgc gcttccgctc cagcatcggg cgcggcagcg tcttgaggtt
     1441 gtcggtgtga acaagggcac cacgagcacc gggccgaacc ctggcggcac cgtgcgtatt
     1501 gtgctggccg acgaccacga cctggtgcgc tccgggctga agctgctgct ggagatggtc
     1561 gcgggcgtcg cggtcgtagg ccaggcccgc aatggcgagg agttgctggc cgaggtgggg
     1621 cgccacgcgc ccgacctggt ggtgaccgac ctgtccatgc ccggcatgga cggcctgacc
     1681 gcgctggccg cactgcgcgc catgccacag ccgccgcgcg tgctggtggt ttcaatgcac
     1741 gactcgcccg acttcatccg ccgtgcgctg caactcggtg ccgcaggcta cgtcatgaag
     1801 gagagctcgc cgctcgaact cgagcatgca gtgcgcagcg tgatggccgg tgtgccgtac
     1861 tacagcgcgc aggtgtcgca gcggctgctt ctggcgcgcg agcgctcgcc agaagaaatg
     1921 ctgaccgagc gccaactcga aatcctggtg ctgatcgcgc tcggtcaggc cgccaaggaa
     1981 atcgccttca agctcagctt gagcccgaag acggtggacg tgcaccgggc acgcatcatg
     2041 gagcggctcg gcatcaatga tctgcccgga ctgaccttgt actgcgtgcg gcatggtctg
     2101 gtcgatccgg tgcgtcacgc ctgaggtgtc aggactcgcg ccccctacag ctgctcgacc
     2161 ggcacgaacg gcgtcagcag ccacgcc
//
LOCUS       DATMJB010000272         2765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_190514, whole genome shotgun sequence.
ACCESSION   DATMJB010000272 DATMJB010000000
VERSION     DATMJB010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190514"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2017
                     /locus_tag="VNU48_06065"
     CDS             <1..2017
                     /locus_tag="VNU48_06065"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HWI10876.1"
                     /translation="DARSLAVIGALGGFATPLLVSSGGGNYVALFSYYLVLDLGIALV
                     AWHRTWRSLNLIGFVATFLVATAWGVLDYRPDRYANSQAFLIAFFVLFVAIMLMPARR
                     AAAPASSDVAQRVDGWVNGSLLFGLPTITFALQYGLVRDTGYGVALSALVLAAFYVLL
                     ALALRSRPRLQLPFEGTLAVGTVFLTLVIPFALDARSTAGAWALEGAGLVWLGWRQRR
                     GISRLFGYTLLLLAGFAMLAGHERHGVPTSVFNAYLFSGLMAAAASLAAAFFVWRHAP
                     RASSAGIDGEALAEPMLIGWGTLWLLAAAALQIDTFVTAPWRPAAWIAVLSAIAAAYV
                     LLCWRLTWRTVSLPAVGHAPLLALATAMTAAWFESPLHHGGWWAWPFALAVHLLVLQR
                     AAPRWPAPLRTLAHTLGVLVLAGLGALQGRAITADWGDAGSAWAWLGWLAVPALLLML
                     LPRAGTAKRWPVSAAPQAYQTIAAAVLAAAALLWSVLANIASDGSAKPLPHLPLLNPL
                     DLGIALALVATALWLRSPGATALSIAPAGRVALIGAAGFIWLNAMLVRGFHHYAGVPY
                     RFDAWAESLAVQTGITLLWSIISLVLMWLAARRAALVPALAGASRAAWMVGAGLLAAV
                     VLKLLIVDLSGTGSVTRIVSFIGVGVLMLVIGYVAPPPGREERHAVV"
     gene            2004..>2765
                     /locus_tag="VNU48_06070"
     CDS             2004..>2765
                     /locus_tag="VNU48_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HWI10877.1"
                     /translation="MPWSDRSGRCGALLALCIAAEVFMPAANAEEPAFRFEAPIAVQR
                     SAPFVELALTGAAYARSRAQGADGGNGPRDLRIVDAGNEPVPFAWLAPRAGQARASES
                     LRDAVLYPLPAKPTADGSWRAPIDVVVEGERIHVRRLSARGTSGDAARSGGWLIDLGE
                     RQPAKPAPDQLRLAWSGPSEFSAAFDIATSDDLRSWRGGGAGQVLALQSAGGALTQPN
                     VPLRADGGRFVRLVWADAATAPALTGAKVVTTQRDT"
BASE COUNT          287 a          996 c         1027 g          455 t
ORIGIN      
        1 ggacgcccgc tcgctcgccg tcatcggcgc gctcggcgga ttcgcgacgc cgctgctggt
       61 gtcgagcggc ggcggcaact atgtcgcgct gttcagctac tacctggtgc tcgacctcgg
      121 cattgcgctg gtggcctggc accggacctg gcgctcgttg aacctgatcg gcttcgtcgc
      181 gaccttcctc gtcgcgacgg catggggcgt gctcgactat cggcccgatc gttacgcgaa
      241 cagccaggcg ttcctgatcg cgttcttcgt gctgttcgtc gcgatcatgc tgatgccggc
      301 gcggcgcgcg gcggcgccgg ccagcagcga cgtcgcgcag cgtgtcgacg gttgggtcaa
      361 cggcagcctg ctgttcggcc tgccgacgat cacgttcgca ctgcagtacg gcctggtgcg
      421 cgataccggg tacggcgtag ccctgtccgc gctggtgctg gcggcgttct atgtactgct
      481 ggccttggcg ctgcgttcgc ggccccgcct gcaactgccg ttcgaaggca cgctcgcggt
      541 cggcacggtg ttcctgacgc tcgtgatccc gttcgcgctc gacgcgcgca gcaccgccgg
      601 cgcctgggcg ctcgaaggag ccggcctggt ctggctcggc tggcgccagc ggcgcggcat
      661 ctcgcgcctc ttcggttaca cgctgctgct gctcgccggc ttcgcgatgc tcgccgggca
      721 cgagcgccac ggcgtgccga cgagcgtctt caacgcgtac ttgttcagcg gcctgatggc
      781 tgccgcagcc tcgctcgcgg cggcgttctt cgtctggcgc cacgcgccgc gcgcgagctc
      841 cgcaggcatc gacggcgaag cgctggccga gccgatgctg atcggctggg gcacgctgtg
      901 gctgctcgcc gcggcggccc tgcagatcga tacctttgtg accgcgccgt ggcgcccggc
      961 ggcatggatc gccgtgctca gcgcgatcgc tgccgcgtac gtgctgcttt gctggcgcct
     1021 gacctggcgc accgtgtcgc tgccggcggt cggccatgcg ccgctgctcg cgctggcgac
     1081 cgcgatgacc gcggcctggt tcgagagtcc gctgcaccac ggcggctggt gggcctggcc
     1141 gttcgcgctc gcggtgcacc tgctggtgct gcagcgcgcc gcgccgcgct ggccggcgcc
     1201 gctgcgcacg ctcgcgcaca cgctcggggt gctcgtgctc gcgggcctcg gcgccctgca
     1261 gggacgggcg atcaccgcgg actggggcga tgccggcagc gcctgggcct ggctcggctg
     1321 gctcgccgtg ccggcactgc tgttgatgct gttgccgcgg gccggcaccg cgaaacgctg
     1381 gccggtcagc gccgcgccgc aggcgtacca gacgattgcg gcggcggtgc tcgcggccgc
     1441 tgcgttgctg tggagcgtgc tcgcgaacat cgcgtccgac ggctccgcga agcccttgcc
     1501 gcatctgccg ctgctgaacc cgctggacct gggcatcgcg ctcgcgctcg tcgcgacggc
     1561 cctgtggctg cgcagcccgg gcgccaccgc gctgagcatc gcgccggccg ggcgcgtcgc
     1621 gctgatcggc gcggccgggt tcatctggct caacgcgatg ctggtgcgcg gcttccatca
     1681 ctacgccggc gtgccgtacc ggttcgacgc gtgggccgaa tcgctcgcgg tgcagaccgg
     1741 catcaccttg ctgtggtcga tcatctcgct cgtgctgatg tggctggccg cgcggcgtgc
     1801 cgcactcgtg ccggcactgg ccggtgcgtc gcgagccgcc tggatggtcg gcgcgggcct
     1861 gctcgccgcc gtcgtgctga agctcctgat cgtcgacctg tcgggcaccg gcagcgtgac
     1921 gcggatcgtc tcgttcatcg gcgtcggcgt gctgatgctg gtgatcggct acgtggcgcc
     1981 gccgcctggc agggaggagc ggcatgccgt ggtctgatcg atcgggtcgt tgcggcgcgc
     2041 tgctcgcgct gtgcatcgcg gcagaggtgt tcatgccggc ggccaatgca gaagagccgg
     2101 cgtttcgatt cgaggcgccc atcgccgtgc agcgcagcgc gccgttcgtc gagctcgcgc
     2161 tgacgggcgc tgcctacgcg cgcagtcgcg cgcagggcgc tgacggcggc aacggcccgc
     2221 gcgacctgcg catcgtcgat gccggcaacg aaccggtgcc gttcgcgtgg ctcgcgccgc
     2281 gcgctggcca ggcgcgcgcg agcgaatcgc tgcgcgacgc cgtgctctat ccgctgccgg
     2341 ccaagcccac ggccgatggc agctggcgcg cgccgatcga cgtcgtcgtc gaaggcgaac
     2401 gcatccacgt gcgccgcctc agtgcgcgcg gcacgagcgg cgacgccgca cgttccggcg
     2461 gctggttgat cgacctcggc gagcgccagc cggcgaagcc ggcccccgac cagctgcgcc
     2521 tcgcctggtc gggcccgagc gagttcagcg ccgcgttcga catcgcgacc agcgacgacc
     2581 tgcgcagctg gcgcggtggc ggcgccggcc aggtgctggc gctgcagtcg gccggcggcg
     2641 cgctgaccca gccgaacgtg ccgctgcgcg cggacggcgg acgcttcgtg cggctcgtct
     2701 gggccgatgc ggcgaccgcg ccggcgctca cgggggcgaa ggtggtgacg acacagcgcg
     2761 acacc
//
LOCUS       DATMJB010000273         3958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_596091, whole genome shotgun sequence.
ACCESSION   DATMJB010000273 DATMJB010000000
VERSION     DATMJB010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596091"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /gene="hflC"
                     /locus_tag="VNU48_06075"
     CDS             <1..654
                     /gene="hflC"
                     /locus_tag="VNU48_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HWI10878.1"
                     /translation="KSLVIDWLVKWRITDPRQFIRNNGSDMRGLETRLSPIVHAAFNE
                     EVTKRTVGEVLSTARDKVMQDVRGRLTDDAKSFGIEILDVRIKRVDFVANITDSIYSR
                     MASERKQVANQLRATGGAEGEKIRADADRQREVIVAEAYRDAQKVKGEGDAKASAIFA
                     DSFGRDAQFAKFYRSLEAYRASFRTKNDVMVVDPGSDFFQSMRGSGAAAPAALAKKK"
     gene            676..864
                     /locus_tag="VNU48_06080"
     CDS             676..864
                     /locus_tag="VNU48_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HWI10879.1"
                     /translation="MWDLLVGAFALMLVIEGLLPFISPPKWRSVFERAMRMTDGQIRF
                     IGLSSMLAGLAMLYFFLS"
     gene            929..2092
                     /locus_tag="VNU48_06085"
     CDS             929..2092
                     /locus_tag="VNU48_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HWI10880.1"
                     /translation="MSSAWLLPEHIADVLPAQARHIEDLRRGLIDVARSYGCELVMPP
                     LLEHIESLLSGAGRTLDLKTFKLVDQLSGRTLGVRADSTPQVARIDAHLLNRAGVTRL
                     CYCGPVLHALPAGLHSTREPLQFGAEIYGHGGLEADLEVLDLTLDCLRHAGLTGVTLD
                     LGDARIVRAVLAGVMVGAEALGEVYAALAAKDAAALRALSRGFPDEVRSALQALVKLF
                     GAAEVLEVARRELPQRPLVQAALADLQGLAQRIEQAHPEVKVGFDLADIGGNGYYSGV
                     RFAAFVPGSPDALARGGRYDEVGAAFGRNRAAVGFSLDLKALAAIAPAQPLHAAIRAP
                     WAEDATLRARVRALREGGETVVRVLPGHEHEAQEFDCDRELVPVGGEWVVRAL"
     gene            2159..3502
                     /locus_tag="VNU48_06090"
     CDS             2159..3502
                     /locus_tag="VNU48_06090"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HWI10881.1"
                     /translation="MNTRADAPGAGHNVVVVGTQWGDEGKGKVVDWLTDHAQAVVRFQ
                     GGHNAGHTLVIKGVKTALQLIPSGIMRDGVACYIGNGVVVDPTHLLGEIERLEKIGVE
                     VRSRLFISESCPLILPFHVEVDKAREALRESSGAGKIGTTGKGIGPAYEDKVARRALR
                     VQDLKHPQRFAKKLHELLELHNFALGGYLRSKPLAYQPIYDHAMQVAEQLRPMMADVG
                     YILHNANRAGANILFEGAQGTLLDIDHGTYPYVTSSNCVAGNAAAGAGVGPDLLHYIL
                     GITKAYTTRVGSGPFPTELPMDEPGTVGHHLSTVGQERGTVTGRPRRCGWLDAALLKR
                     SVIINGISGLCITKLDVLDGLPEIKVCVGYELHGKRIDILPLDADDIVACVPIYDSFE
                     GWTDSTFGVTEWDKLPLNARRYLERVQGLIGAPIDMVSTGPDRVHTILLRHPYQD"
     gene            3575..>3958
                     /locus_tag="VNU48_06095"
     CDS             3575..>3958
                     /locus_tag="VNU48_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWI10882.1"
                     /translation="MLTDDGKHLYVSWDEYHMLIERLALKIAASGWQFDQILCLARGG
                     MRPGDVLSRVFDKPLAIMATSSYRAEAGTIQGRLDLAKYITLPRGELAGRVLLVDDLA
                     DSGITLRAVVERLRGMPSISELKSAV"
BASE COUNT          619 a         1375 c         1372 g          592 t
ORIGIN      
        1 aagagcctgg tgatcgactg gctcgtgaaa tggcgcatca ccgacccgcg ccagttcatc
       61 cgcaacaacg gctcggacat gcgcggcctc gagacccgcc tgagcccgat cgtgcacgcg
      121 gccttcaacg aagaggtgac caagcgcacc gtcggcgagg tgctgtcgac cgcacgcgac
      181 aaggtcatgc aggacgtgcg cggccggctg accgacgacg ccaagtcctt cggcatcgag
      241 atcctggacg tgcgcatcaa gcgcgtcgac ttcgtcgcca acatcaccga ttcgatctac
      301 agccgcatgg cctcggagcg caagcaggtc gcgaaccagc tgcgcgccac cggcggcgcc
      361 gagggcgaga agatccgcgc tgacgccgac cgccagcgcg aggtgatcgt cgccgaggcc
      421 tatcgcgatg cgcagaaggt caagggcgag ggcgatgcga aggcctcggc gatctttgcc
      481 gattcgttcg gccgcgatgc ccagttcgcc aagttctatc gcagcctcga ggcctaccgg
      541 gcgagcttcc gcaccaagaa cgatgtgatg gtcgtcgacc cgggcagcga cttcttccag
      601 tccatgcgcg gctccggagc ggcggcgccg gcggcgctcg cgaagaagaa gtaggcggtg
      661 gcgtcctcgc cgagcctgtg ggacctgctg gtcggtgcgt tcgcgctgat gctggtgatc
      721 gagggcctgc tgccgttcat cagcccgccg aagtggcgca gtgtgttcga gcgcgcgatg
      781 cgcatgaccg acgggcagat ccgcttcatc ggcctgtcga gcatgctcgc cgggctggcg
      841 atgctctact tcttcctgtc gtgagcgggc gacgaaaccg gcgaagcgcg ccggtacaat
      901 gccgttttta accccccggc gttttcacat gtcctctgct tggctgctgc ccgagcacat
      961 tgccgatgtg ctgcccgcgc aggcgcggca catcgaagac ctgcggcgcg gcctgatcga
     1021 cgtggcgcgc agctacggtt gcgagctcgt gatgccgccg ctgctcgagc acatcgagtc
     1081 actgctgtcg ggcgccggcc gcacgctcga cctgaagacc ttcaagctcg tcgaccagct
     1141 cagtggccgc acgctcgggg tgcgcgccga cagcacgccg caggtcgcac gcatcgacgc
     1201 gcacctgctg aatcgcgccg gcgtgacgcg cctgtgctac tgcggcccgg tgctgcatgc
     1261 gctgccggcg ggcctgcact cgacccgcga gccgctgcag ttcggtgccg agatctacgg
     1321 ccacggcggc ctggaggccg acctcgaggt gctggacctg acgctcgact gcctgcgcca
     1381 cgccgggctg accggcgtga cgctcgacct cggcgacgcg cgcatcgtgc gcgcggtgct
     1441 ggccggcgtg atggtgggtg ccgaggcgct cggcgaggtc tatgcggcgc tcgcggcgaa
     1501 agacgcggcg gcgctgcgcg cgctgtcgcg cggtttcccc gacgaggtgc gctctgcgct
     1561 gcaggcgctc gtcaagctct tcggtgcggc cgaagtcctc gaggtggcgc ggcgcgaact
     1621 gccgcagcgg ccgctggtgc aggccgcgct cgccgatctg caggggctgg cccagcgcat
     1681 cgagcaggcg caccccgagg tcaaggtcgg cttcgacctc gccgacatcg gcggcaacgg
     1741 ctactacagc ggggtgcgct tcgcggcgtt cgtgccgggc tcgccggacg cgctcgcgcg
     1801 cggcggccgc tacgacgagg tcggcgccgc cttcggtcgc aaccgcgcgg cggtcggctt
     1861 cagcctggac ctgaaggcgc tcgccgcgat cgcgccggcg cagccgttgc acgcggcgat
     1921 ccgcgcaccg tgggccgagg acgcgacgct gcgcgcccgc gtgcgcgcgc tgcgcgaagg
     1981 cggcgaaacc gtggtgcgcg tgctgcccgg tcatgagcac gaggcgcagg aattcgactg
     2041 cgatcgcgag ctcgtgccgg tgggtggcga gtgggtcgtg cgcgcgctct gagcgcgggc
     2101 ttgacgcagc aatgctcccc gagagattcc agttttggac gactttgaca tgcaagcaat
     2161 gaacacgcgc gccgacgcgc cgggcgccgg ccacaacgtc gtcgtcgtcg gcacccagtg
     2221 gggcgacgaa ggcaagggca aggtggtcga ctggctcacc gaccacgccc aggcggtggt
     2281 gcgcttccag ggcggccaca acgccggtca cacgctggtg atcaagggcg tgaagacggc
     2341 gctgcagctg atcccgtcgg gcatcatgcg cgacggtgtc gcctgctaca tcggcaacgg
     2401 cgtggtggtc gatccgacgc acctgctcgg cgagatcgag cggctcgaga agatcggcgt
     2461 cgaggtgcgc tcgcgcctgt tcatcagcga gtcgtgcccg cttatcctgc ccttccacgt
     2521 cgaggtcgac aaggcgcgcg aggcgctgcg cgagagcagc ggcgccggca agatcggcac
     2581 caccggcaag ggcatcggcc cggcctacga agacaaagtc gcgcgccgcg cgctgcgcgt
     2641 gcaggacctg aagcacccgc agcgcttcgc gaagaaactg cacgaattgc tggagctgca
     2701 caacttcgcg ctcggcggct acctgaggtc caagccgctc gcgtaccagc cgatctacga
     2761 ccatgcgatg caggtcgccg agcagctcag gccgatgatg gccgacgtcg gctacatcct
     2821 gcacaacgcg aaccgggccg gcgccaacat cctgttcgag ggcgcacagg gcacgctgct
     2881 cgacatcgac cacggcacct atccgtacgt cacgtcgagc aactgcgtgg ccggcaacgc
     2941 ggccgcgggt gccggcgtcg ggcccgacct gctgcactac atcctgggca tcacgaaggc
     3001 ctacaccacg cgtgtgggca gcgggccgtt cccgaccgag ctgccgatgg acgagcccgg
     3061 cacggtcggc caccacctgt cgacggtcgg ccaggaacgc ggcaccgtga ccggccggcc
     3121 gcgacgctgc ggctggctcg acgccgcgct gctgaaacgt tcggtcatca tcaacggcat
     3181 ctcggggctg tgcatcacca agctcgacgt gctcgacggc ctgcccgaga tcaaggtctg
     3241 cgtcggctac gaactgcacg gcaagcgcat cgacatcctg ccgctggacg ccgacgacat
     3301 cgtggcctgc gtgccgatct acgacagctt cgagggctgg accgacagca ccttcggcgt
     3361 caccgaatgg gacaagctgc cgctgaacgc gcgccgctac ctcgagcgcg tgcagggcct
     3421 gatcggcgcg ccgatcgaca tggtgtccac gggcccggac cgcgtccaca cgatcctgtt
     3481 gcgccatccg taccaggact gagcagcgct tcgctatgct tcgggccggc gcccgttcca
     3541 tcacctccac cgcaccgatc ctgaagggtt tcgcatgctg accgacgacg gcaaacacct
     3601 gtacgtgagc tgggacgagt accacatgct catcgagcgg ctcgcgctga agatcgccgc
     3661 ctcgggctgg cagttcgacc agatcctgtg cctggcgcgc ggcggaatgc gccccggcga
     3721 cgtgctgtcg cgcgtcttcg acaagccgct cgcgatcatg gcgacgagct cgtaccgcgc
     3781 cgaggccggg acgatccagg ggcggctcga cctggccaag tacatcacgc tgccgcgcgg
     3841 cgagctcgcc ggccgcgtcc tgctcgtcga cgacctcgcc gactcgggga tcaccctgcg
     3901 cgccgtcgtc gagcgcctgc gcggcatgcc gtcgatcagc gagctgaagt cggcggtg
//
LOCUS       DATMJB010000274         3379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_859393, whole genome shotgun sequence.
ACCESSION   DATMJB010000274 DATMJB010000000
VERSION     DATMJB010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859393"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VNU48_06100"
     CDS             complement(<1..644)
                     /locus_tag="VNU48_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWI10883.1"
                     /translation="MLGAVSHAQQVLRVTAIPDESPTELARKAAPLVKYLEARLGMKV
                     EFTPVSDYAAAVETLINRKVDLAWFGGFTFVQASARSGGKVIPIVQREEDERFRSVFI
                     TSDPAIKTLADLKGKDVSFGAQSSTSGHLMPRSALLQAGVDPDKDFKRVAYSGAHDAT
                     IAAVAAGKVQAGALNISVWEKFVADKRVDTDKVRVFFTTPPYYDYNWSVGAEMP"
     gene            871..1941
                     /gene="selD"
                     /locus_tag="VNU48_06105"
     CDS             871..1941
                     /gene="selD"
                     /locus_tag="VNU48_06105"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HWI10884.1"
                     /translation="MNPPPSSPSGPRLTSLSHGGGCGCKIAPGLLGEILKNTNRLPMP
                     PELLVGIETADDAAVYQLTDEIALIATTDFFMPIVDDPFDFGRIAAANAISDVYAMGG
                     KPIMALALVGMPINVLSTEVIGRILEGGESVCRAAGIPIAGGHTIDSVEPIYGLVALG
                     VVHPKRVKRNADARIGDRLILGKPLGVGVLSAALKKESLSAEGYAQMITTTTQLNTPG
                     PDLAAMDGVHALTDVTGFGLAGHALELARGAQAAVALDWSRVPLLAGVRELAQRGMVT
                     GASDRNWAGYGSEVKLPAGFSALDRALLTDPQTSGGLLVSCDASAVGDVMAVFRRHGF
                     AAAAEIGEIVAESGSGARLQVR"
     gene            complement(1982..2884)
                     /locus_tag="VNU48_06110"
     CDS             complement(1982..2884)
                     /locus_tag="VNU48_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI10885.1"
                     /translation="MFQPNLMAGQRILVTGGGTGLGQAMAERFLGLGADVAICGRRQS
                     VLDETAAAWRAQFPERRVDTFGVDIRNAQAVEEMVESLWVGGGLTGLVNNAAGNFVAP
                     TESLSPRAFDAIAGIVFHGSFYVTQAIGKRWVADAKSGAWQRGQQMRNVMSIIVTWVE
                     NGGPYVVPSAMSKAGIEVMTRSLATEWARYGIRLNAIAPGEIPTEGMNKRLNPGEQPG
                     ERTIRSSPMARPGQKSELQNLATFLMAPGQCDWLTGQSITLDGATALATGGNFYELRE
                     WSDADWQAARERIEAQNAKDKTQR"
     gene            3028..>3379
                     /locus_tag="VNU48_06115"
     CDS             3028..>3379
                     /locus_tag="VNU48_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HWI10886.1"
                     /translation="MSGLDQRLRELPDARLREMRRGVEKESLRALPDGSLALTPHPAA
                     LGSALTHPHITTDFSESQLEFVTGVHASAQACLDELTQIHQFSYRALRELGDEMLWVS
                     SMPCGLPTDENIPIG"
BASE COUNT          489 a         1165 c         1177 g          548 t
ORIGIN      
        1 tcgggcatct cggcgccgac cgaccagttg tagtcgtaat acggcggtgt cgtgaagaag
       61 acgcggacct tgtcggtgtc gacgcgcttg tcggccacga acttctccca caccgagatg
      121 ttgagcgcac ccgcctgcac cttgccggcc gcgaccgccg cgatggtcgc gtcgtgcgcg
      181 ccgctgtagg ccacgcgctt gaagtccttg tcggggtcga cgccggcctg cagcagcgcg
      241 ctgcgcggca tcaggtggcc gctggtcgag ctctgcgcac cgaagctcac gtcctttccc
      301 ttcaggtcgg ccagggtctt gatcgccggg tcggacgtga tgaacaccga gcggaaacgt
      361 tcgtcttcct cgcgctgcac gatcgggatc accttgccgc cggagcgtgc cgacgcctgc
      421 acgaaggtga agccgccgaa ccaggcgagg tcgaccttgc ggttgatcag cgtctcgacc
      481 gctgcggcgt agtcgctcac cggcgtgaac tcgaccttca tgccgagcct ggcttcgagg
      541 tacttgacca gcggcgccgc cttgcgcgcg agttcggtcg gcgactcgtc ggggatcgcg
      601 gtgacgcgca gcacctgctg cgcgtgcgaa accgcgccca gcatcaggaa caggccggcg
      661 aggaacagtc gcggcagcga cgagcgaaaa gaagtcagca tggggtggac gtggttgatt
      721 cgggaagccc gcattacacc gcacgcgcgt tcgcgtgcgc cacgcggcga tactggacgc
      781 attgcgcgca gcgcgcgcgc cactgttcga gccggcgcgc gagcgcgcga cgctgctgtc
      841 cttgttgaac gacctgccgg agcatcctca gtgaacccac caccatcgtc cccgtccggc
      901 ccgcgcctga cctcgctgtc ccacggcggc ggctgcggct gcaagatcgc gccgggcctg
      961 ctcggcgaga tcctgaagaa cacgaaccgg ctgccgatgc cgccggaact gctggtcggc
     1021 atcgagaccg ccgacgacgc ggcggtctac cagctgaccg acgagatcgc gctgatcgcg
     1081 accactgact tcttcatgcc gatcgtcgac gacccgttcg atttcggccg catcgccgcg
     1141 gcgaacgcga tcagcgatgt ctacgcgatg ggcggcaagc cgatcatggc gctcgcgctg
     1201 gtgggcatgc cgatcaacgt gctgtcgacg gaggtgatcg gccggatcct cgaaggcggc
     1261 gaatcggtgt gccgcgccgc cggcatcccg atcgcgggcg gccacacgat cgactcggtc
     1321 gagccgatct acggcctggt ggcgctgggc gtggtgcatc cgaagcgcgt caagcgcaac
     1381 gccgacgcgc gcatcggcga tcgcctgatc ctgggcaagc cactgggggt gggcgttctg
     1441 tcggcggcgc tgaagaagga gtcgctgtcg gccgagggct acgcgcagat gatcaccacc
     1501 accacgcagc tgaacacgcc ggggcccgac ctggccgcga tggacggcgt gcatgcgctg
     1561 accgacgtga ccggcttcgg cctggcgggc catgcgctgg aactggcgcg cggcgcgcag
     1621 gccgcggtcg cgctcgactg gtcgcgcgtg ccgctgctgg ccggcgtgcg cgaactcgcg
     1681 cagcgcggca tggtcaccgg cgcctcggat cgcaactggg ccggctacgg ttccgaggtg
     1741 aagctgcccg ccggcttctc ggcgctggat cgggccttgc tcaccgaccc gcagaccagc
     1801 ggcggcctgc tggtctcgtg tgacgccagt gcggtcggcg atgtgatggc ggtgttccgc
     1861 cgccacggct tcgctgcggc cgcggagatc ggcgagatcg tcgccgagtc gggcagcggc
     1921 gcgcgtctgc aagtgcgctg agcgggcgcg acagcgcaga cggcacgctc gaacatcgcc
     1981 ctcagcgctg cgtcttgtcc ttcgcgttct gcgcctcgat gcgctcgcgc gcggcctgcc
     2041 agtcggcgtc gctccattcg cgcaactcgt agaagttgcc gccggtggcg agcgcggtgg
     2101 cgccgtccag cgtgatgctc tggccggtga gccagtcgca ttgccccggt gccatcaaga
     2161 aggtcgcgag gttctgcagc tcgctcttct gccccggccg cgccatcggg ctgctgcgga
     2221 tggtgcgctc gcccggctgc tcgccggggt tcagccgctt gttcatgcct tcggtcggga
     2281 tctcgcccgg cgcgatcgcg ttcaggcgga tgccgtagcg cgcccactcg gtcgccagcg
     2341 acctggtcat cacctcgata ccggccttgc tcatcgccga gggcacgacg tacgggccgc
     2401 cgttctcgac ccaggtgacg atgatgctca tcacattgcg catctgctgc ccgcgctgcc
     2461 acgcgcccga tttcgcgtcg gcgacccagc gcttgccgat cgcctgcgtg acgtagaagc
     2521 tgccgtggaa gacgatgccc gcgatcgcgt cgaacgcgcg cggcgagagg ctctcggtcg
     2581 gcgcgacgaa gttgcccgcg gcgttgttga ccaggccggt caggccgccg ccgacccaca
     2641 ggctctcgac catctcctcg accgcctggg cgttgcggat gtcgacgccg aaggtgtcga
     2701 cgcggcgttc ggggaactgt gcgcgccagg ccgcggcggt ttcatcgagg accgattggc
     2761 gccggccgca gatcgccaca tcggcgccca ggcccaggaa acgctcggcc atggcttggc
     2821 ccaggccggt gccgccgccg gtgaccagaa tgcgctggcc ggccatcagg ttgggttgga
     2881 acattgttgt ctcctcgttt tttcgggtcg ggcgaggcta tcaccgcggc accgggcttg
     2941 tccacccggc gacgggttcc cgatcgccgg caggcaaatt caaggtctgt taccgtccgc
     3001 cccaagagag ttctgaggag aaaccgcatg agcggtctgg accaacgcct gcgcgaattg
     3061 cccgatgcgc ggctgcgcga gatgcgccgg ggggtggaga aggagagcct gcgtgcgctg
     3121 ccggacggca gccttgcgct gaccccgcac ccggccgcgc tcggctcggc gctgacgcac
     3181 ccgcacatca cgaccgattt cagcgagtcg cagctcgagt tcgtcaccgg cgtgcacgcc
     3241 agcgcgcagg cctgcctgga cgagctgacg cagatccacc agttcagcta ccgtgcgctg
     3301 cgcgagctcg gcgacgagat gctgtgggtc tcgagcatgc cctgcgggct gccgaccgac
     3361 gagaacatcc cgatcggcc
//
LOCUS       DATMJB010000275        11693 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_774387, whole genome shotgun sequence.
ACCESSION   DATMJB010000275 DATMJB010000000
VERSION     DATMJB010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11693
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774387"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="VNU48_06120"
     CDS             complement(<1..509)
                     /locus_tag="VNU48_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWI10887.1"
                     /translation="MLLSLADWLQTLSPQFGFFRVFQYLTFRAVMAAMTSMLIGLAFG
                     PWVIARLTSLKIGQPVRAYGVESHMLKTGTPTMGGVLILIGIGVSTLLWFDWSNRFVW
                     IVMLVTIGFGAIGWVDDWRKVVQKNPEGMRSREKYFWQTLIGLIAALYLAFSVSETSN
                     LRVLELFIR"
     gene            complement(499..1974)
                     /locus_tag="VNU48_06125"
     CDS             complement(499..1974)
                     /locus_tag="VNU48_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HWI10888.1"
                     /translation="MMTLAQAHALLPGSTLVGDAATVVARVHSDTRSLQRGDLFVALK
                     GERFDAHDFLAQAKVSGAVAAIAERGLDAAGLPGLQVADTQRSLTALARAWRQRFQLP
                     LIAVTGSNGKTTVTQMIATILRAWLGDAAFATTGNLNNHIGVPLTLLRLRQDESAWHR
                     AGVVELGMNHPGEIAELAAITAPTVALVNNAQREHQEFMASVEAVAHENGAAIAALGA
                     TGTAVFPADDAHAPLWQQLAGQRPTLTFALSGPADVMCHAEWVDGAATGRSLAAPTPS
                     GGGLGLPSPGGSMPHWAVLLQTPAGASTVQLQVAGWHNVKNALAATACALAAGCPLDA
                     IRRGLEAFVPVKGRSQVRRFERDGRSVTLIDDSYNANPDSVRAAIEVLASLPGPRWLV
                     LGDMGEVGDQGPAFHREVGAFAKARGVDALWTVGEASTHAAAAFAGARHFDTVASLLA
                     ALPQAPACAAVLVKGSRFMKTEQVVAALLATASDAGGAHAA"
     gene            complement(1971..3581)
                     /locus_tag="VNU48_06130"
     CDS             complement(1971..3581)
                     /locus_tag="VNU48_06130"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWI10889.1"
                     /translation="MALQSSIRQLDSVAEALGWLAQRGVRALATDSRKVVAGDAFIAW
                     PGYAVDARQFVPQALAAGASACLIEADGAPGFDAIDDPAAVAALHGLKAATGPLASGF
                     MGAPSEQLIVAAVTGTNGKTSTTWWVAQALSALGRRCGVIGTLGVGEPPSPRQASAIT
                     FTGMTTPDPVTVQAGFKRFADAQFQACAIEATSIGIEEQRLAGTKIDVALFTNFTQDH
                     LDYHGSMEAYWQAKARLFAWPGLRAAVINVDDAQGARLARSLQDTAIDLWTYSTQGNA
                     RLHALKVGYAAGGLAFELHEGAARVEVKTQLIGDYNVANLLATIGGLRACGVALTDAA
                     AACALLTPVPGRMQRVNVDPPVAEYRDRPPEGASPGLGRPGARARADASGPQVVVDYA
                     HTPDALEKALLALQPFAAERGGQLWCVFGCGGNRDATKRPLMGALAQRLAQQVVITSD
                     NPRHEAPAFILLQIVAGLKGSQERVTVIEDRRAAIAHAVRHAGARDVILVAGKGHEDY
                     QEIRGVKHPFSDVAEAGAALRLREGAPS"
     gene            complement(3581..5410)
                     /locus_tag="VNU48_06135"
     CDS             complement(3581..5410)
                     /locus_tag="VNU48_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWI10890.1"
                     /translation="MSWRDSIKSNGAGKTGRSPNRSGASSVRSVTYSTSPLLASKTPP
                     WRSKFLVAVVGLSFCGLLGRALYVQVIGTDFFQRQGEIRYARTLDLPASRGRILDRNG
                     LILASSVPAPSLWVDPKDLDPDPAQRKQLAKLLGMTGKELADRLDDNPNFVWLRRQVD
                     DHVAKAVLALNLKGVHQVREYKRKYPEGEAATHVVGFTNVEDHGQEGIELAYEADLFG
                     RNGTRRVIKDRLGRVVEDIGDSVAPVDGKDIDLSIDSKVQFFAYQRIRDAVAENKAKA
                     GSVVVLDARSGEVLALANFPSYTPADRKNLTGAQLRNRALTDTFEPGSTVKPFIAAWA
                     LETGRVKPDTIVHTAPGRISMGGFVISDSHPHGDLTVSEVIQKSSNIGAMRMGLMFQP
                     REMWELYTQAGFGQKPQLGFPGAVTGRLRPYKTWRPIEQATMSYGYGLSASLFQIAHA
                     YTVFAHDGEMIPVTLTKSAASGDDARVAGLRVISPDTAKTIRQMLSLVTGPGGTAPKA
                     QTIGYSVGGKSGTAYKQEGKGYATKKYRAWFVGIAPIVNPRIIVAVMVDEPSAGKWYG
                     GDVAAPVFSDLVQQTLRTLNVQPDLDVKTQIIAKDVPAEEESF"
     gene            complement(5407..5700)
                     /gene="ftsL"
                     /locus_tag="VNU48_06140"
     CDS             complement(5407..5700)
                     /gene="ftsL"
                     /locus_tag="VNU48_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HWI10891.1"
                     /translation="MTRLNALLLLALIASAVYLVRVSHESRRLFAELDRARSEERLLD
                     NEFERLKSDKQSQATPLRVEKTARDKLAMRSATPAVTQYISRAEASGAAGSAR"
     gene            complement(5697..6731)
                     /gene="rsmH"
                     /locus_tag="VNU48_06145"
     CDS             complement(5697..6731)
                     /gene="rsmH"
                     /locus_tag="VNU48_06145"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWI10892.1"
                     /translation="MDLQSDRPHTTVLLNEAVDALVTTPDGTYLDGTYGRGGHSRLLL
                     SRLSAKGRLVAIDRDPQAIVAATEGATRVSDPRFSIHHTSFAHIVPLLSSLGVAQVDG
                     LLLDLGVSSPQIDDPERGFSFRFDGPLDMRMDTTRGESAADFLARADVRQMTEVIRDY
                     GEERFALQVAKALVARRESGNPVRTTGELSNVVAGAVKTREAGQNPATRTFQALRIFV
                     NAELEELEQGLNASLGLLAPGGRLVVISFHSLEDRIVKTFIARESKHAFDRRAPFAAP
                     QAMRLRALGRVKPGDDEVRANPRARSAVMRVAERTEVPAGGPSAGPLPSRHASPRGTG
                     RGTSADEGLQ"
     gene            complement(6783..7211)
                     /locus_tag="VNU48_06150"
     CDS             complement(6783..7211)
                     /locus_tag="VNU48_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HWI10893.1"
                     /translation="MFQGPAALTLDGKGRIAVPARHRELLTAMGVSQLTVTKHPEGSL
                     LIFPRPAWETFRDKVAALPMEAAGWKRVFLGNAMDVDIDASNRVLIAPELRASAGLVR
                     DVMLLGMGSHFELWDAKAHAEHESRVMQSEMPESLKSFSF"
     gene            complement(7505..9253)
                     /locus_tag="VNU48_06155"
     CDS             complement(7505..9253)
                     /locus_tag="VNU48_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWI10894.1"
                     /translation="MNNFKLSTRLTLGFGVLALLIAFTAGMALARLPTIESAFHKVTN
                     ERIPRVAALNEVKGNLDQIALSMRNMAIMSDVAERKKQVEQILAARKTIGDDLDRLKT
                     EIASEKGKMLLAKVFDARAAYVPAQAKFIDLVDAGKADEAKAHLMGVARPLQLAYFAT
                     VDELIQLQGELTKESSAEANAAMTAIKSAMWVSAGIALLAALAMAVWIIRSVMRQLGA
                     DPADAAALAQSVAAGDLSVRIDLRPGDTTSLMAQLKEMQTSLSSVVSSVRGNSESVAT
                     ASAQIAQGNLDLSQRTEEQASALEETAASMEQLSSTVKQNADNARQANQLALGASTVA
                     IKGGEVVGQVVETMKGINDSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGRG
                     FAVVASEVRSLAGRSAEAAKEIKGLISASVERVEQGTALVDQAGVTMTEVVSSIRRVT
                     DIMGEISAASTEQSQGVAQVGEAVSQMDQVTQQNAALVEESAAAAESLKNQAQQLVQA
                     VAVFKLSQGKQSDSAAAPMVRAPGVKSPAAVERRGPGRAKNVTRPTFGTKARAELPTL
                     TEAARAKTGTDDWESF"
     gene            complement(9645..11168)
                     /locus_tag="VNU48_06160"
     CDS             complement(9645..11168)
                     /locus_tag="VNU48_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWI10895.1"
                     /translation="MNFRSKMWAIPAAAACLLGLGLVVTTVLGWRTSSQLEQLQRASY
                     PYLEQISRIDRALDNMRASLQSAAMEGDADKLGEAKAHAERIEAALAAAAALAGHEAQ
                     ASALRQAFTEYKNAGMATVAALLAKQPADDKLKLMQATQKQLVASLKEQDDAARATVN
                     QGFVEVAASRTRSDWVNIAVALAAMLGLGLAARGVVASVWRDLGGEPAVLRDRVQRVA
                     AGDLTTDIRVDANDRGSLNAAVASMTQRLRDAVSQIRGASDSISTASGEIAAGNQDLS
                     ARTEDTASNLQRASSELEQITGSVRHSAEAAQQANQLAAEAATAAQRGGEIVSSVVDS
                     MSGISQASGKIGEIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRTLAQ
                     RSAQAAREIKTLIQTSGEKVQSGTRLVTDAGQAMAEIVAGVRRVSDIIGEINTAMTQQ
                     SSGIEQVNRAVSELEQMTQQNAALVEQSAAAAQSMNTQASELVGSVSAFRLEAIAAA"
     gene            complement(11199..>11693)
                     /locus_tag="VNU48_06165"
     CDS             complement(11199..>11693)
                     /locus_tag="VNU48_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10896.1"
                     /translation="RALQRRVGDRRFRLLRGSIMKNKLNMNVMRLVAATLVSLPLIAQ
                     AGVAVVVGAKSPATKLNAEQVAQLFLAKTPTLPGAGQAVLVDQAEGSAIRDAFYNKVA
                     GKSAAQVKALWSRLVFSGAAQSPKALSSSAEVKKLLAENPDAIGYIDSAEVDASVKVL
                     LTVD"
BASE COUNT         1699 a         4080 c         3968 g         1946 t
ORIGIN      
        1 cagcggatga agagttcgag cacgcgcagg ttggacgtct cgctgacgct gaacgcgagg
       61 tacagcgccg cgatcaggcc gatcagcgtt tgccagaagt acttctcgcg cgagcgcatg
      121 ccttcggggt tcttctgcac caccttgcgc cagtcgtcga cccagccgat cgcgccgaag
      181 ccgatcgtca ccagcatcac gatccagacg aaacggttgc tccagtcgaa ccagagcagc
      241 gtggacacgc cgatgccgat caggatcagc acgccgccca tcgtcggcgt gccggtcttg
      301 agcatgtgcg actcgacacc gtaggcgcgc accggctggc cgatcttcag cgacgtgagc
      361 cgcgcgatca cccacgggcc gaacgccagg ccgatcagca tcgaggtcat cgccgccatc
      421 accgcgcgga aggtcaggta ctggaagacg cggaagaagc cgaactgcgg cgacagcgtc
      481 tgcagccagt cggccaggct aagcagcatg cgcgcctcct gcgtcggatg cggtcgccag
      541 cagcgccgcc accacctgct cggtcttcat gaagcgcgag cccttgacca gcaccgcggc
      601 gcaggccggc gcctgcggca gtgcggccag caggctcgcc acggtgtcga aatgccgcgc
      661 ccccgcgaac gccgcggccg cgtgcgtgct ggcctcgccg acggtccaca gcgcatcgac
      721 gccgcgcgcc ttcgcgaacg cgccgacctc gcgatggaac gccgggccct ggtcgccgac
      781 ctcgcccatg tcgcccagca ccagccagcg cgggcccggc agcgacgcga gcacctcgat
      841 cgcggcgcgc accgagtccg ggttggcgtt gtagctgtca tcgatcagcg tgaccgaccg
      901 gccgtcgcgc tcgaagcgcc tgacctgcga gcggcccttg accggcacga aggcctcgag
      961 cccgcgccgg atcgcgtcca gcgggcagcc ggcggccagc gcgcaggccg tcgcggcgag
     1021 cgcgttcttg acgttgtgcc agcctgcgac ctgcagctgc acggtgctcg cgcccgccgg
     1081 cgtctgcagc agcacggccc agtgaggcat ggaacccccc gggctcggca agccgagccc
     1141 gccccccgag ggggtcgggg ccgctaggga gcggcccgtc gcggccccgt cgacccattc
     1201 ggcgtggcac atcacgtcgg ccgggccgct cagcgcaaag gtcaacgtcg ggcgctggcc
     1261 ggcgagctgc tgccagagcg gcgcgtgcgc gtcgtcggcc gggaacaccg cggtgcccgt
     1321 cgcgcccagc gccgcgatcg ccgcgccgtt ctcgtgcgcg accgcctcga cgctggccat
     1381 gaactcctgg tgctcgcgct gcgcgttgtt gacgagcgcg acggtcggcg cggtgatcgc
     1441 ggccagttcg gcgatctcgc ccgggtggtt catgccgagt tcgacgacgc cggcgcggtg
     1501 ccaggcgctt tcgtcctggc gcagccgcag cagcgtcagc ggcacgccga tgtggttgtt
     1561 caggttgccc gtggtcgcga aggcggcgtc gccgagccag gcgcgcagga tcgtcgcgat
     1621 catctgcgtc accgtggtct tgccgttgct gcccgtgacc gcgatcagcg gcagctggaa
     1681 gcgctggcgc caggcgcgcg ccagcgccgt cagcgagcgc tgcgtgtccg cgacctgcag
     1741 gcccggcagg ccggccgcgt cgaggccgcg ttcggcgatc gccgcgaccg cgccgctgac
     1801 cttggcctgc gcgaggaagt cgtgcgcgtc gaagcgttcc cccttcaacg cgacgaacag
     1861 gtcgccgcgc tgcaggctgc gcgtgtcgct gtgcacgcgc gcgacgacgg tcgcggcgtc
     1921 gccgaccagc gtcgagcccg gcagcagcgc gtgggcctgc gcgagcgtca tcatgatggc
     1981 gccccttcgc gcaggcgcag cgccgcgccc gcttcggcca cgtccgagaa cgggtgcttg
     2041 acgccgcgga tctcctggta gtcctcgtgg cccttgccgg cgaccaggat cacgtcgcgc
     2101 gcgccggcgt ggcgcaccgc gtgcgcgatc gcggcgcgcc gatcctcgat cacggtgact
     2161 ctttcctggc tgcccttcag ccccgcgacg atctgcagca ggatgaaggc cggcgcttcg
     2221 tggcgcgggt tgtcgctggt gatcacgacc tgctgcgcca ggcgctgcgc gagcgcgccc
     2281 atcagcggcc gcttggtcgc atcgcggttg ccgccgcagc cgaacacgca ccagagctgc
     2341 ccgccgcgct cggcggcgaa gggctgcagc gcaagcagcg ccttctcgag cgcatccggc
     2401 gtgtgcgcat agtcgacgac gacctgcggc cccgacgcgt cggcacgagc ccgagcgccg
     2461 ggccgcccca agccgggcga ggccccctcg gggggtcgat cgcggtactc cgcgaccggg
     2521 gggtcgacat tcacccgctg catgcggccg ggcaccggcg tcagcaacgc gcaggccgcg
     2581 gccgcgtcgg tcagcgcgac accgcaggcg cgcaggccgc cgatcgtcgc gagcaggttc
     2641 gcgacgttgt agtcgccgat cagctgcgtc ttcacctcga cccgcgccgc gccctcgtgc
     2701 agctcgaagg ccaggccgcc ggcggcgtag cccaccttga gtgcgtgcag ccgcgcgttg
     2761 ccctgggtcg aataggtcca caggtcgatt gcagtgtcct gcagcgagcg cgcgagccgt
     2821 gcgccctgcg cgtcgtcgac gttgatcacc gcggcgcgca ggcccggcca ggcgaacagg
     2881 cgcgccttgg cctgccagta cgcctccatc gagccgtggt agtcgaggtg gtcctgggtg
     2941 aagttggtga acagcgcgac gtcgatcttc gtcccggcga ggcgctgctc ttcgatgccg
     3001 atcgaggtcg cctcgatcgc gcaggcctgg aactgcgcgt cggcgaagcg cttgaagccg
     3061 gcctgcaccg tgaccgggtc gggcgtggtc atgccggtga aggtgatggc cgacgcctgc
     3121 cgcggcgacg gcggctcacc gaccccgagc gtgccgatca cgccgcagcg ccggcccagc
     3181 gcgctcaagg cctgcgcgac ccaccaggtg gtcgaggtct tgccgttcgt cccggtcacc
     3241 gcggccacga tcagctgctc gctcggtgcg cccatgaagc cgctcgccag cggtccggtg
     3301 gcagccttca ggccgtgcag cgcggcgacc gcggccgggt cgtcgatcgc atcgaacccc
     3361 ggcgcgccgt cggcctcgat cagacaggcg ctcgcaccgg ccgccagcgc ctgcggcacg
     3421 aactggcggg cgtcgaccgc gtagcccggc caggcgatga acgcgtcgcc cgcaacgacc
     3481 ttgcggctgt cggtcgcgag cgcgcgcacg ccgcgctgcg cgagccagcc cagcgcctcg
     3541 gcgacggaat cgagctgacg gatcgacgac tgaagcgcca tcagaaactc tcctcctcgg
     3601 ccggcacgtc tttcgcgatg atctgcgtct tgacgtcgag atcgggctgc acgttgagcg
     3661 tgcgcagggt ctgctgcacc aggtcgctga acaccggcgc ggcaacatcg ccgccgtacc
     3721 atttgccggc gctgggctcg tcgaccatca ccgcgacgat gatgcgcggg ttgacgatcg
     3781 gcgcgatgcc gacgaaccag gcgcggtact tcttggtcgc gtagcccttg ccctcctgct
     3841 tgtacgcggt gcccgacttg ccgccgaccg agtagccgat ggtctgcgcc ttcggcgcgg
     3901 tgccgccggg cccggtgacg agcgaaagca tctgccggat cgtcttcgcg gtgtccggcg
     3961 agatcacgcg caagcccgcg acgcgcgcgt cgtcgccgga cgccgcgctc ttggtcagcg
     4021 tcaccgggat catctcgccg tcgtgcgcga acacggtgta ggcatgcgcg atctggaaca
     4081 gcgacgccga caggccgtag ccgtagctca tcgtcgcctg ctcgatcggc cgccaggtct
     4141 tgtaggggcg caaccgcccg gtcaccgcac cggggaagcc gagctgcggc ttctggccga
     4201 agccggcctg cgtgtacagc tcccacatct cgcgcggctg aaacatcagg cccatgcgca
     4261 tcgcgccgat gttgctcgac ttctggatca cttcgctcac cgtcaggtcg ccgtgcgggt
     4321 gcgagtcgct gatcacgaag ccgcccatcg agatgcgccc cggcgcggtg tggacgatgg
     4381 tgtcgggctt gacgcggccg gtctcgagcg cccacgcggc gatgaagggc ttgacggtgg
     4441 agccgggctc gaaggtgtcg gtgagcgcac ggttgcgcag ctgcgcgccg gtcaggttct
     4501 tgcgatcggc cggcgtgtag ctcgggaagt tcgccagcgc cagcacctcg ccgctgcgcg
     4561 cgtcgagcac gacgacgctg cccgccttgg ccttgttctc ggcgaccgcg tcacggatgc
     4621 gctggtaggc gaagaactgc accttcgagt cgatgctcag gtcgatgtcc ttgccgtcga
     4681 cgggtgcaac gctgtcgccg atgtcctcga cgacgcggcc gagccggtcc ttgatgacgc
     4741 ggcgcgtgcc gttgcggccg aacaggtcgg cctcgtacgc gagctcgatg ccctcctggc
     4801 cgtggtcctc gacgttggtg aagccgacca cgtgggtcgc ggcctcgccc tcggggtact
     4861 tgcgcttgta ctcgcgcacc tggtgcacgc ccttcaggtt cagcgcgagc acggccttgg
     4921 cgacatggtc gtcgacctgt cggcgcagcc agacgaagtt cgggttgtcg tcgagccggt
     4981 cggcgagctc cttgccggtc atgccgagca gcttcgcgag ctgcttgcgc tgcgccggat
     5041 cggggtccag atccttcggg tccacccaca gcgacggcgc cggcacgctc gacgcgagga
     5101 tcagcccgtt gcggtcgaga atgcgcccgc ggctggccgg cagatcgagc gtgcgcgcgt
     5161 agcggatctc gccctggcgc tggaagaagt cggtgccgat cacctgcacg tagagcgcac
     5221 ggccaagcag tccgcagaag ctcaggccga cgacggcgac gaggaatttc gagcgccacg
     5281 ggggcgtctt cgacgccagc aacgggctgg tcgaataggt cacgctgcgc acgctggaag
     5341 cgccgctgcg attcggcgag cggccggtct tgccggcgcc gttgctcttg atgctgtccc
     5401 gccagctcat cgcgcgctcc cggccgcacc cgacgcttcg gcacggctga tgtactgcgt
     5461 gacggccggc gtcgcgctgc gcatcgcgag cttgtcgcgc gcggtcttct cgacgcgcaa
     5521 cggcgtcgcc tgcgactgct tgtcggactt caggcgctcg aactcgttgt cgagcagccg
     5581 ctcctcgctg cgcgcccggt caagttcggc gaacagccgg cgcgactcgt gcgagacgcg
     5641 caccaggtag accgcgctgg caatcagcgc gagcagcagc aacgcattca ggcgcgtcat
     5701 tggagcccct cgtccgcgga cgtaccgcgg ccggtccccc ggggggacgc gtgccggctt
     5761 gggagcggcc cggcgctcgg cccgcctgcc ggcacctcgg ttctctcggc gacgcgcatc
     5821 accgcggacc gcgcgcgcgg gttcgcgcgc acttcgtcgt cgccgggctt gacgcggccg
     5881 agcgcgcgca gccgcatcgc ctgcggcgca gcgaacggcg cgcgccggtc gaacgcatgc
     5941 ttgctttcgc gcgcgatgaa ggtcttgacg atgcggtctt cgagcgagtg aaagctgatc
     6001 acgaccagcc gcccgcccgg ggccagcagc ccgagcgacg cgttcaggcc ttgttcgagc
     6061 tcctcaagct cggcgttgac gaaaatccga agagcttgaa atgtgcgcgt tgcagggttc
     6121 tggcccgctt cgcgggtctt gaccgcacca gccacgacgt tggaaagctc ccctgtggtt
     6181 cgaacagggt tcccgctttc gcgccgagca acaagcgcct ttgcaacctg tagagcaaac
     6241 cgttcttccc catagtcacg tatcacctcc gtcatttggc gcacgtcggc gcgggccagg
     6301 aaatctgccg cgctctcgcc gcgggtggtg tccatgcgca tgtccagcgg gccgtcgaaa
     6361 cggaagctga agccacgttc ggggtcgtcg atctgcggcg acgagacgcc caggtcgagc
     6421 agcaggccgt cgacctgggc gacgcccagc gacgagagca gcggcacgat gtgcgcgaag
     6481 ctggtgtgat gaatggaaaa acgcgggtcc gaaacccgcg ttgcgccttc ggttgccgcc
     6541 acgatcgcct gcgggtcacg gtcgatggcc accagccttc ccttggccga caggcgcgac
     6601 agcagcaggc gcgagtggcc gccgcgccca taggtgccgt cgaggtaggt gccgtcaggc
     6661 gtcgtcacga gggcatccac cgcttcgttg agcagcacgg tcgtgtgcgg acggtcgctc
     6721 tggaggtcca tgaacggtgg caggagcggg agaagtgcag agaaggaacc aggcgcaaac
     6781 gctcagaagc tgaagctctt gagcgactcg ggcatctcgg actgcatcac gcgggattcg
     6841 tgctcggcgt gggccttggc gtcccacaac tcgaagtggc tgcccatgcc gagcagcatc
     6901 acatcacgca ccaggccggc cgacgcgcgc agctcggggg cgatcagcac gcgattcgac
     6961 gcgtcgatgt cgacgtccat cgcattgccg aggaacaccc gcttccagcc cgccgcttcc
     7021 atcggcagcg ccgcgacctt gtcgcgaaag gtctcccacg ccgggcgcgg gaagatcagc
     7081 aacgagcctt cgggatgctt ggtgacggtc agctggctga cgcccatcgc ggtcaggagc
     7141 tcgcggtgac gtgccggcac ggcgattcgc cccttgccgt ccaacgtcag ggccgcaggc
     7201 ccttgaaaca caatgtttgc cacaaatatc caagaaagtg cactttgtgc cactttaacg
     7261 gatggttttg ggccggtcaa tcaattccgg caagaaaaat caatggaatc aaagacttag
     7321 cggcgatttg aaacgagttc cgtcgcgaaa attcgttcaa aaacaacgac ctgcgcaggc
     7381 cactgaaagt aatgggttag atgagcgggc gctttgcgtc cggcaaatct gggctctccg
     7441 acgagcgctc accaggtcgg agtcccgccg aagcggggct ccgtctccga ggtcaggctg
     7501 acgatcagaa gctttcccag tcgtcggtgc cggtcttggc gcgcgcggct tcggtcaagg
     7561 tcggcaactc ggccctggcc ttggttccga acgtcggccg cgtcacattc ttggcgcgac
     7621 cgggaccgcg ccgctcgacg gccgcggggc tcttgacccc gggggcgcgc accatgggcg
     7681 ccgccgcact gtcgctctgc ttgccttggc tgagcttgaa caccgccacc gcctgaacca
     7741 gttgctgcgc ctggttcttc aggctctcgg cggcggcggc actttcctcg accagcgcgg
     7801 cgttctgctg cgtgacctgg tccatctggc tcaccgcctc gccgacctgc gccaccccct
     7861 ggctctgctc ggtgctggcg gcgctgatct cgcccatgat gtcggtcacc cgcctgatcg
     7921 agctgacgac ttcggtcatc gtcacgccgg cctggtcgac gagcgccgtg ccctgctcga
     7981 cgcgctcgac gctggcgctg atcaggccct tgatctcctt ggccgcttcc gcactgcggc
     8041 cggccaggct gcgcacttcg gacgccacga ccgcgaagcc tcgaccttgc tcgcccgcgc
     8101 gcgcggcctc gaccgcggcg ttcagggcca ggatgttggt ctggaaggcg atgccatcga
     8161 tcacgctgat gatgtcggcg atcttcttcg agctgtcgtt gatgcccttc atcgtctcga
     8221 cgacctggcc gacgacctca ccacccttga tcgccaccgt cgatgcgccc agcgccagtt
     8281 gattggcctg tcgggcgttg tcggcgttct gcttgacggt cgagctcagc tgttccatcg
     8341 acgccgcggt ctcttcgagc gcgctggcct gctcctcggt gcgctggctc aggtccaggt
     8401 tgccctgagc gatctgcgcc gatgcggtgg ccacggactc ggaattgccg cgtacgctgg
     8461 agaccacgct ggacaggctg gtctgcatct ccttgagctg cgccatcagg ctggtggtgt
     8521 cgcccggcct gaggtcgatg cgaacgctga ggtcgcctgc ggcgacgctt tgtgccagag
     8581 cggctgcatc ggccgggtcg gcgccgagct ggcgcatcac cgagcggatg atccagacgg
     8641 ccatggccag cgccgccagc agcgcaatcc cagccgaaac ccacatcgcg ctcttgatcg
     8701 cggtcatcgc ggcattggct tcggcgctgg attccttggt cagctcgccc tgcagctgta
     8761 tcagctcgtc gaccgtcgcg aagtaggcca gttgcagcgg gcgcgcgacg cccatcaggt
     8821 gggccttggc ctcgtcggcc ttgcccgcgt cgaccaggtc gatgaacttg gcctgcgccg
     8881 gaacgtaggc cgcgcgtgcg tcgaagacct tggccagcag catcttgccc ttctcgctcg
     8941 cgatctcggt cttcagcctg tccaggtcgt cgccgatggt tttgcgtgcg gccagaatct
     9001 gttccacctg cttcttccgc tcggccacgt cgctcatgat cgccatgttg cgcatcgaca
     9061 gggcgatttg atcgaggttg cccttgacct cgttcagcgc cgccactctg gggatgcgct
     9121 cgttggtcac cttgtggaac gcgctttcga tggtcggcag cctcgccaac gccatgccag
     9181 cggtgaaagc gatcagcagg gccagaacac cgaacccgag ggtcaggcgt gtggacagtt
     9241 tgaagttgtt catggggcgt cgagtccttt gttccgatca ctgcagcggc tgatcgtcta
     9301 tcacgttgtc ggtgccgaac cgctcgactt gatccctgga ttggtgccat gcgccaaagg
     9361 cgtgcatggt ggacataccg cagcacgttc aacggaaacg tcgccgcctg ctcggaaggc
     9421 gtgaatgaat ccggcgtgcc cgggcgggcc gcgaaagcgt gcccaatcaa ggcgcgaagg
     9481 acagccgtat ctcgggctgc gtcgatgaag cgcgccgtct cgcagatgaa catgagcgag
     9541 gtgaccatcg cgtaccgcgc gcccgcacgg gggtggtgct cgaaaggcgt ctcgcgagcc
     9601 gtcgacctcc tcgggcacga cgccccgaca gactccgggg ggcgctacgc tgccgcgatg
     9661 gcctccaggc ggaacgccga caccgagccc acgagttcac tggcctgggt gttcatgctt
     9721 tgggccgccg cagccgactg ttcgaccagc gcagcattct gctgggtcat ttgttcgagt
     9781 tccgacacgg ctcgattgac ctgctcgatg ccgctcgatt gctgcgtcat ggccgtgttg
     9841 atttcaccga tgatgtcgct gacgcggcga accccggcga cgatctccgc catggcctgg
     9901 cccgcgtcgg tgaccaggcg ggtgccgctc tggaccttct cgcccgaggt ctggatcagg
     9961 gtcttgatct ctcgcgccgc ctgggcgctg cgctgcgcca gggtacgcac ttcgcccgcc
    10021 acgaccgcga aaccgcggcc ctgctccccg gcgcgcgcgg cttcgacggc cgcgttgagc
    10081 gcgaggatgt tggtctggaa ggcgatgccg tcgatcacgc cgatgatctc gccgatcttg
    10141 cccgacgcct ggctgatgcc cgacatgctg tcgaccaccg acgagacgat ctcaccgccg
    10201 cgctgtgcgg ccgtcgccgc ctcggcggcc agctggttcg cttgctgggc cgcctctgcg
    10261 ctgtggcgga ccgagccggt gatctgctcg agttcgctgg acgcgcgctg caggttggag
    10321 gcggtgtctt cggtgcgcgc gctcaggtcc tgattgccgg cggcgatctc gcccgaggcc
    10381 gtcgagatcg aatcgctggc gccgcggatc tgcgacaccg catcgcgcaa gcgctgcgtc
    10441 atcgacgcca ccgctgcgtt gagcgacccg cggtcgttcg catcgacgcg gatgtcggtc
    10501 gtcaggtcgc cggccgccac tcgctggacg cgatcgcgca gcaccgcagg ctcgccaccg
    10561 aggtcgcgcc acaccgaggc gaccacgcct cgcgccgcca ggcccaggcc cagcatcgct
    10621 gccagcgcca ccgcgatgtt cacccaatcg ctgcgcgtgc ggctggccgc cacctcgacg
    10681 aagccctggt tgacggtggc gcgcgccgca tcgtcctgct ctttgaggct ggccacgagt
    10741 tgcttctgcg tggcctgcat cagcttgagc ttgtcgtcag cgggttgttt ggccagcagc
    10801 gcggccacgg tggccatgcc ggcgttcttg tactccgtga aggcctgccg caacgccgag
    10861 gcctgcgcct cgtgcccggc cagcgcggcg gccgcagcca gcgcggcttc gatgcgctcg
    10921 gcgtgggcct tggcctcgcc gagcttgtcg gcgtcgccct ccatggccgc cgactggagc
    10981 gacgcgcgca tgttgtccag cgcacggtcg atgcgcgaga tctgctcgag gtaggggtag
    11041 ctggcgcgtt gcagctgctc gagctgagaa ctggtgcgcc agcccaggac ggtggtcacc
    11101 accagaccca ggccgagcag acacgccgca gcagccggga tcgcccacat tttggatcga
    11161 aagttcattc ttcttccttc tgcgacgggt cacccgactc aatcgacggt caggagcacc
    11221 ttgaccgagg catccacttc ggcgctgtcg atgtagccga tggcatcggg gttctcggcc
    11281 aacagcttct tcacttcagc gctgctggac agcgccttgg gcgattgcgc cgcaccgctg
    11341 aacacgaggc gcgaccacag tgccttgacc tgcgcggcgc tcttgcccgc gaccttgttg
    11401 tagaaggcgt cgcgaatggc cgagccttcg gcttgatcga cgagaaccgc ctggcccgcg
    11461 cccggcagcg tcggcgtctt ggcgaggaac aactgggcga cctgttcggc gttgagcttg
    11521 gttgcggggc tcttcgcgcc caccacgacg gccacgccag cctgcgcgat caagggaagg
    11581 ctcaccaagg tcgccgcaac caggcgcatc acgttcatat tgagtttgtt cttcatgatc
    11641 gatcccctca gaagacgaaa tcgacggtcg ccgacacgac gttgtagcgc cct
//
LOCUS       DATMJB010000276         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_443124, whole genome shotgun sequence.
ACCESSION   DATMJB010000276 DATMJB010000000
VERSION     DATMJB010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443124"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VNU48_06170"
     CDS             complement(<1..617)
                     /locus_tag="VNU48_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HWI10897.1"
                     /translation="MNLIYVGDAMCSWCYGFAKPLDALLADPQDCAPLQMALVMGGLR
                     PFTTEPMAPERADELAGHWHRVADASGQSFAQAPHTALHQPGFVYDTEPAARATVTVR
                     SLWPQQVWRYFKAVQHAFYAEGRNVVEPGVLADVAVELGLPRADFGKAFASQKMRDAT
                     LQDFQQSQAWGIRGFPTLVAEHGDHLHLVGSGFMPIATLRDRLANV"
     gene            879..1445
                     /locus_tag="VNU48_06175"
     CDS             879..1445
                     /locus_tag="VNU48_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWI10898.1"
                     /translation="MKFSRSILGTAAALLLSASAMSAHAIDCNPNFTNNGQSPSFSNL
                     SGVTVTQCAGFYGGNAINAGNAGSLALVNGILTGWGLASVTGWIEKQDTNNPLINFDT
                     MLYGDTIVAFHWGNFPGPAGNVSALYRFDAGSAGVDTFTVASAMGLSNAAVYMTSTPA
                     VPEPETYALMFAGLGVVGFVARRRKQPA"
     gene            1522..2202
                     /locus_tag="VNU48_06180"
     CDS             1522..2202
                     /locus_tag="VNU48_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10899.1"
                     /translation="MRALVSTPRQSVSRRGEGSGIFDAQGEFKEMGAIIRCTALLVML
                     QAAPWAHAQVYRCSSGGSTYLSDRPCQVLPKTQLGGYGPVAPGTYVPYSAPLPDAPRV
                     QDHVKYLGSACASISEAIRTGPTRGVRTDVIRGLQEEYSQKCSVEDQEARRQAQQDKV
                     QELQGKLAERDNAVRERQQVKARLDYCSGMRDVIGLKRQRESSLNATEVEALRNLEKA
                     YNARCIGQ"
BASE COUNT          389 a          769 c          804 g          348 t
ORIGIN      
        1 acattggcaa gccgatcgcg cagcgtcgca atcggcatga agccgctgcc gacgaggtgc
       61 aggtgatcgc cgtgctcggc caccagcgtc ggaaagccgc gtatgcccca ggcctgcgac
      121 tgctggaagt cctgcaaggt cgcgtcgcgc atcttctgcg acgcgaacgc cttgccgaag
      181 tcggcgcgcg gcaggccgag ttccacggcg acatcggcga gcacgccggg ctcgacgacg
      241 ttgcggccct cggcatagaa cgcgtgctgc actgccttga agtagcgcca gacctgctgc
      301 ggccacaggc tgcgcaccgt cacggtggcg cgcgcggccg gttcggtgtc gtagacgaag
      361 ccgggctggt gcagcgcggt gtggggcgct tgcgcgaacg actggccgct cgcgtcggcg
      421 acgcggtgcc agtggccggc gagttcgtcg gcgcgttcgg gcgccatcgg ctcggtggtg
      481 aacggccgca gcccgcccat caccagtgcc atctgcagcg gcgcgcagtc ctgcggatcg
      541 gcgagcaacg cgtccagcgg cttcgcgaag ccgtagcacc acgagcacat cgcgtcgccg
      601 acgtagatca ggttcatcaa ggtcgtcctc gaaatcgggt gcggccactc tagcggaatc
      661 gcgcgctgcg ggctggcgcg ggggctctct cgtgcgcgcc ccggctcgaa tgcgacgaag
      721 cgagaaggag ttctcgattc gcgcgttgcg catcccgcgg ctcgggggga ccgaccgcac
      781 gggtcagcct cgcacaattc catcaagccg gatcgagtcc acgagacacg gagcaacgag
      841 cggcgaagct cacaaccgtt tgcccccagg gagtcctcat gaaattttcg cgttccatcc
      901 tcggcaccgc tgcagcgctg ctgctgtctg cgtcggccat gtccgcccat gcgatcgact
      961 gcaaccccaa cttcacgaac aacggccagt cgccgtcctt ctcgaacctg agcggcgtca
     1021 cggtcacgca gtgcgcgggg ttctacggcg gcaacgccat caacgccggc aacgcgggct
     1081 cgctggcgct ggtcaacggc atcctgaccg gctggggcct ggcctcggtg acgggctgga
     1141 tcgagaagca ggacacgaac aacccgctga tcaacttcga cacgatgctg tacggtgaca
     1201 cgatcgtcgc gttccactgg ggcaacttcc ccggcccggc gggcaacgtg agcgcgctgt
     1261 accggttcga tgcgggcagt gccggcgtcg acaccttcac ggtggcctcg gccatgggcc
     1321 tgtcgaacgc ggccgtctac atgacatcga cgccggccgt gccggaaccc gagacctacg
     1381 cgctgatgtt cgccggcctg ggcgtcgtcg gcttcgtggc gcggcggcgc aagcagccgg
     1441 cctgagcggc gcgcgccgcg tccgcgacag ccccgcgcga gcggggcttt tcgttggcgg
     1501 cagcggcgtc ggaagaacgc ggtgcgcgcg cttgtctcga caccccgcca atcggtgtcg
     1561 cgacgcggcg aaggatcggg tatcttcgac gctcagggag agttcaaaga aatgggcgca
     1621 atcattcgat gcacagccct gctcgtcatg ctccaagcgg ccccatgggc gcacgcgcag
     1681 gtttaccgtt gcagcagcgg cggctcgacg tatctctcgg atcggccctg tcaggtcctg
     1741 ccgaagacgc agctcggcgg ctatgggccg gtggctccgg gcacttacgt tccgtactct
     1801 gcgccgctgc cggacgcgcc gcgcgtgcag gatcacgtca agtacctagg ttcggcgtgc
     1861 gcctcgatca gcgaggcgat ccgcaccggc ccgacgcgtg gtgtgcgcac ggacgtcatc
     1921 cgggggctgc aggaagagta cagccagaag tgttcggtcg aagaccagga ggcgcggcgc
     1981 caagcccaac aggacaaagt ccaggaactg cagggcaagc tggccgagcg cgacaacgcg
     2041 gtgcgggaac gccagcaggt caaggcccga ctcgactact gcagcggcat gagggacgtg
     2101 atcggcctga agcgccagcg tgagagcagc ctgaatgcca ccgaggtcga ggcgctgcgc
     2161 aacctggaga aggcgtacaa cgcgcggtgc atcggccaat aggctgagcg caacggtgcc
     2221 gcgatcgcgg gcaccttgag ttcaggctgc ggcaaagctg acatcgatgg cgtgccgggc
     2281 cgcggccagc gcgttcttcg cggccaggtc
//
LOCUS       DATMJB010000277         2779 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_446539, whole genome shotgun sequence.
ACCESSION   DATMJB010000277 DATMJB010000000
VERSION     DATMJB010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446539"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /gene="serC"
                     /locus_tag="VNU48_06185"
     CDS             <1..698
                     /gene="serC"
                     /locus_tag="VNU48_06185"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HWI10900.1"
                     /translation="IPKRETWKLDPNAAYVHICANETIGGVEYHFVPDVGDVPLIADV
                     SSNILSRPLDVARFGLIYGGAQKNIGPAGLTIVVVRDDLLGAALPITPSAFNYAEQAA
                     NDSMLNTPPTYAIYIAGLVFHWIKAQGGLTAMEAHNRAKAALLYDYLDSTGFYSSPIV
                     REDRSLMNVPFKLKDASLDAAFLKGAEARGMVQLKGHRSVGGMRASIYNAMPVEGVKA
                     LVAYMKEFEASHG"
     gene            691..1905
                     /locus_tag="VNU48_06190"
     CDS             691..1905
                     /locus_tag="VNU48_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWI10901.1"
                     /translation="MADAPGAARFQVLVLNQISANGLARLPAGRYSVGKAIASPDAVL
                     VRSADMHAMEIAPSVQAIARAGAGTNNIPVAAMSARGVPVFNAPGANANAVKELVLAA
                     MLLTARNLVPALRFVAALDAQAPGMEKAVEDGKKNFAGFELAGHTLGIVGLGKVGCLV
                     ADAAIKLGMQVHGFDPHITVDAAWSLPAQVRKAASVADVLQRADFVSLHVPLIDATRD
                     LVNADNIGLMRPGAVLLNFSREGVVNEAAVLAALDAPNAAKRLGWYACDFPSAAVHAH
                     PRVVALPHLGASTAEAEENCAIMVVEQLRDYLEHGNVENAVNFPQVSMARESAYRVAI
                     ANANVPNMLGQISTAMANAGLNIHNMVNKSRGDMAYTLVDVDSAVNASVLDSIRAIDG
                     VLSVRYLPHMPG"
     gene            1999..>2779
                     /locus_tag="VNU48_06195"
     CDS             1999..>2779
                     /locus_tag="VNU48_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HWI10902.1"
                     /translation="MPKTKTPARPAHAFAKTRKTFKTASGKEGTFYSLPALAKEFPNV
                     SRLPVAIRIVLESVLRNCDGQKVTKEHVAELANWRPNAERSNEIPFVVARVVLQDFTG
                     VPLLADLAAMRNVAAAMGKNPKTIEPLVPVDLVVDHSIMIDHYGKKNSLDLNMKLEFQ
                     RNNERYQFMKWGMQAFDTFGVVPPGFGIVHQVNLEYLARGVHKAKDTLTKETVYYPDS
                     LVGTDSHTTMINGIGVVGWGVGGIEAEAGMLGQPVYFLTPDV"
BASE COUNT          472 a          964 c          931 g          412 t
ORIGIN      
        1 gcatccccaa gcgcgagacc tggaagctcg acccgaacgc cgcctacgtg cacatctgcg
       61 cgaacgagac catcggcggc gtcgagtacc acttcgtgcc cgatgtcggc gacgtgccgc
      121 tgatcgccga cgtgtcctcg aacatcctgt cgcggccgct ggacgtggcc cgcttcggcc
      181 tgatctacgg cggcgcgcag aagaacatcg gcccggccgg gctgacgatc gtcgtcgtgc
      241 gcgacgacct gctcggcgcg gcgctgccga tcacgccgtc ggccttcaac tacgccgagc
      301 aggccgccaa cgattcgatg ctgaacacgc cgccgaccta cgcgatctac atcgccggcc
      361 tggtcttcca ctggatcaag gcccagggcg gcctgaccgc gatggaggcg cacaaccgcg
      421 cgaaggccgc gctcttgtac gactacctcg attcgaccgg cttctacagc agcccgatcg
      481 tgcgcgaaga ccgctcgctg atgaacgtgc cgttcaagct gaaagacgct tcgctcgacg
      541 ccgcgttcct gaaaggcgcc gaggcgcgcg ggatggtcca gctcaagggc caccgctcgg
      601 tcggcggcat gcgcgcgtcg atctacaacg cgatgccggt cgagggcgtg aaggcgctgg
      661 tggcctacat gaaggagttc gaggcctccc atggctgacg ctcccggcgc ggcgcgcttc
      721 caggtgctgg tgctgaacca gatctcggcc aatggcctgg cgcggctgcc ggccggacgc
      781 tacagcgtcg gcaaggcgat cgcatcgccc gacgcggtgc tggttcgatc cgccgacatg
      841 cacgcgatgg agatcgcacc cagcgtgcag gcgatcgcgc gcgccggcgc cggcaccaac
      901 aacatcccgg tcgctgcgat gagtgcgcgc ggcgtgccgg tgttcaacgc gccgggcgcg
      961 aacgcgaacg cggtcaagga actcgtgctc gcggcaatgc tgctgaccgc gcgcaacctc
     1021 gtgcccgcgc tgcgcttcgt cgccgcgctc gatgcgcagg cgccgggcat ggagaaggcc
     1081 gtcgaggacg gcaagaagaa cttcgccggc ttcgagctgg ccggccacac gctgggcatc
     1141 gtcggcctgg gcaaggtcgg ttgcctggtc gccgatgcgg cgatcaagct cggcatgcag
     1201 gtccacggct tcgacccgca catcaccgtc gacgcggcct ggagcctgcc ggcgcaggtg
     1261 cgcaaggcgg cgagcgtggc cgacgttctt cagcgcgccg acttcgtcag cctgcacgtg
     1321 ccgctgatcg acgcgacccg cgacctcgtc aacgccgaca acatcggact gatgcggccc
     1381 ggcgcggtgc tgctgaactt ctcgcgcgag ggggtcgtca acgaggccgc ggtgctggcc
     1441 gcgctcgacg cgccgaacgc ggcgaagcgc ctgggctggt atgcctgcga cttcccgagt
     1501 gccgcggtcc acgcgcaccc gcgcgtggtc gcgctgccgc acctgggcgc gtccaccgcc
     1561 gaggccgagg agaactgcgc gatcatggtc gtcgagcagc tgcgcgacta cctcgagcac
     1621 ggcaacgtcg agaacgcggt caacttcccg caggtcagca tggcgcgcga gtcggcctac
     1681 cgcgtcgcga tcgcgaacgc gaacgtgccg aacatgctgg gccagatctc caccgcgatg
     1741 gccaacgccg ggctgaacat ccacaacatg gtcaacaagt cgcgcggcga catggcctac
     1801 acgctggtcg atgtcgacag cgcggtgaac gcctcggtgc tggactcgat ccgcgcgatc
     1861 gacggcgtgc tgtcggtgcg ctacctgccg cacatgccgg gctgaatcga gggccgcacg
     1921 cccgtcatcg tctgctgaca caatgccctt ttgccctgcg ccggccgcgt ttgccggcgc
     1981 ttcaacgagg tgcccgccat gcccaagacc aagactcccg cccgccccgc ccacgccttt
     2041 gccaagacgc gcaagacctt caagaccgcg tccggcaagg agggcacgtt ctactcgctg
     2101 ccggcgctgg cgaaggagtt cccgaacgtg agccggctgc cggtggcgat ccgcatcgtg
     2161 ctcgaatcgg tgctgcgcaa ctgcgacggc cagaaggtga cgaaggagca cgtcgccgag
     2221 ctcgcgaact ggcggccgaa cgccgaacgc agcaacgaga tcccgttcgt cgtcgcccgc
     2281 gtcgtgctgc aggacttcac cggcgtgccg ctgctggccg acctggccgc gatgcgcaac
     2341 gtcgccgcgg cgatgggcaa gaacccgaag acgatcgagc cgctggtgcc ggtcgacctg
     2401 gtcgtcgatc actcgatcat gatcgaccac tacggcaaga agaactcgct cgacctgaac
     2461 atgaagctcg agttccagcg caacaacgag cgctaccagt tcatgaagtg gggcatgcag
     2521 gccttcgaca ccttcggcgt cgtgccgccg ggcttcggca tcgtgcacca ggtgaatctg
     2581 gagtacctcg cgcgcggcgt gcacaaggcg aaggacacgc tgacgaaaga gaccgtctac
     2641 taccctgaca gcctggtcgg taccgacagc cacacgacga tgatcaacgg catcggcgtg
     2701 gtcggctggg gcgtcggcgg catcgaggcc gaggcgggga tgctcgggca gccggtgtat
     2761 tttctgacgc ccgacgtgg
//
LOCUS       DATMJB010000278         4069 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_222958, whole genome shotgun sequence.
ACCESSION   DATMJB010000278 DATMJB010000000
VERSION     DATMJB010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4069
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222958"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VNU48_06200"
     CDS             <1..350
                     /locus_tag="VNU48_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWI10903.1"
                     /translation="AGLPAPAVTGTPPAARDPAAILADKPASAPKLAAGADPFLYFVQ
                     AGAYGRTEDAEQQRAKLAMLGVEGKLTERDQAGRTVYRVRIGPFERKDDAEAMKAKLG
                     DAGVEAALVRVQR"
     gene            437..1087
                     /locus_tag="VNU48_06205"
     CDS             437..1087
                     /locus_tag="VNU48_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HWI10904.1"
                     /translation="MKRRAFSTTLLGAGTAALAWPGLVQAQGEPIEGRQYVRLTQPVP
                     VGAEPGKIEVIDFFWYGCPHCNAFEPTLEAWVRQLPADVAFRRVPVAFSAEPFAAHQR
                     IFYALDGLGLVAAMHRKVFYAIHGERLRLDQPAEISDFMARNGVDAARFMEAYGSFGT
                     QARLRQANQLVAGYKIDGVPALGIHGRFYTSGTLAGDNSRALAVTDFLVQRLRKPV"
     gene            1152..1820
                     /gene="lptA"
                     /locus_tag="VNU48_06210"
     CDS             1152..1820
                     /gene="lptA"
                     /locus_tag="VNU48_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HWI10905.1"
                     /translation="MPNLTSRHCLRLALTGLALALGCAAPARAERADRLQKINIAADH
                     DGQIDVRNQVVVYNGNVVISQGTMVIRAARIEMRELPNGYRTAVAFGAPNKPATFRQK
                     RDGVDEYFEGEAERLEYDGQSNTVRFIRSAQLRQLRGATTANQINGNLITYDATTEKM
                     TVSGGAAPTAANPGGRVSAVLTPREGSEAAAEIAAAASAAASAPLKLSPALSAPAPAA
                     KDKR"
     gene            1817..2593
                     /gene="lptB"
                     /locus_tag="VNU48_06215"
     CDS             1817..2593
                     /gene="lptB"
                     /locus_tag="VNU48_06215"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HWI10906.1"
                     /translation="MSLTAGAEIHPREHRLEASGLRKSYGARTVVKDVHLAVRSGEVV
                     GLLGPNGAGKTTSFYMIVGLVRADAGEIKIEGQRVERLPIHRRSRLGLSYLPQEASIF
                     RKLNVEENIRAVLELQVDADDKALKPALIEELLDGLLRDLSIDKLRASPAPSLSGGER
                     RRVEIARALATQPRFILLDEPFAGVDPIAVIEIQRIIGFLKSRGIGVLITDHNVRETL
                     GICDRAYIISEGRVLAEGTPTEIVDNADVRKVYLGEHFRM"
     gene            2717..>4069
                     /gene="rpoN"
                     /locus_tag="VNU48_06220"
     CDS             2717..>4069
                     /gene="rpoN"
                     /locus_tag="VNU48_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HWI10907.1"
                     /translation="MKPTLQFRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLESN
                     PFLETDDDAGPAFEPLAERTTPAERVGERETERAAETGSDGVGNDEAAAPLDAAEFGG
                     TERDDWENGTEREDFDGIRETPGKSSASPDGEDFDGADRDTAIPSLQDHLREQLSSLR
                     LSASDAAAVMVLIESLNDDGYLADSLEDIATQLTAGEDDPEAEREALLERLRCALRYL
                     QSMEPTGVGARDLGECLTLQLRNRPRSEAQAIAIIVCKQHLELLARRDLKKLCAVTGA
                     DEELLREAQALIVALEPKPGRQFARAEANIIIPDVIVQKAGRGWKVTLNPDVMPKLRI
                     NDLYASAIRQQRGAGSRGVPDALNGAGAGLNSRLQEARWFMKNILQRFDTIQRVSQAI
                     VERQKNFFTHGAIAMKPLVLREIADELGLHESTISRVTTAKYMSTPFGTFELKYFFGS
                     S"
BASE COUNT          666 a         1474 c         1396 g          533 t
ORIGIN      
        1 cggcgggcct gccggcgccg gccgtgaccg gcacaccgcc cgcggcgcgc gacccggccg
       61 cgatcctggc cgacaagccg gcctcggcac cgaagctggc cgccggggcc gacccgttcc
      121 tgtacttcgt ccaggccggc gcctatggcc gcaccgagga cgccgaacaa cagcgcgcca
      181 agctggcgat gctgggcgtc gagggcaagc tgaccgagcg cgatcaggcc ggccgcaccg
      241 tgtaccgcgt gcgcatcggc ccgttcgagc gcaaggatga cgccgaggcg atgaaggcca
      301 agctcggcga cgccggcgtc gaggccgcgc tggtgcgggt gcagcggtag agaaggtgcc
      361 gcgggcctga actttgctca cccgatccga ctcagcctgt cacccgtctt accgcagccc
      421 accggcctcc aaccccatga aacgtcgcgc gttctcgacc accctgctcg gcgcgggcac
      481 cgcggcgctc gcgtggccgg gcctggtgca ggcgcagggc gagccgatcg agggccggca
      541 gtacgtccgg ctgacccagc cggtgccggt cggcgcggaa cccggcaaga tcgaggtgat
      601 cgacttcttc tggtacggct gcccgcactg caatgcgttc gagccgacgc tggaagcctg
      661 ggtccggcag ctgccggccg atgtggcgtt ccggcgcgtg ccggtcgcgt tcagcgccga
      721 gcccttcgcc gcccaccagc gcatcttcta cgcgctcgac ggcctgggcc tggtcgctgc
      781 gatgcaccgc aaggtcttct acgcgatcca cggcgagcgc ctgcggctgg accagccggc
      841 cgagatctcg gacttcatgg ccaggaatgg cgtcgacgcg gccaggttca tggaggccta
      901 cggctcgttc ggcacccagg ccaggctgcg ccaggcgaac cagctggtcg ccggctacaa
      961 gatcgacggt gttccggcgc tgggcatcca cggccgcttc tatacctcgg gcaccctggc
     1021 cggcgacaac agccgggccc tggccgtgac cgacttcctg gtacagcgcc tgcgcaagcc
     1081 cgtttgacgg catcttgcgg gccggggccc ggaatgcgtg ggctagaatg gcctgcaagt
     1141 ttcatgccct tatgccgaac ctcacttcac gccactgcct gcgccttgcc ctgaccgggc
     1201 tcgcgctcgc cctcgggtgc gccgcaccgg cccgcgccga gcgcgccgac cggctgcaga
     1261 aaatcaacat tgccgctgac catgacggcc agatcgacgt gcgcaaccag gtcgtcgtct
     1321 acaacggcaa cgtcgtcatc agccagggca cgatggtgat ccgggccgca cgcatcgaga
     1381 tgcgcgagct gcccaacggc tatcgcacgg cggtcgcctt cggcgcgccg aacaagcccg
     1441 cgacctttcg ccagaagcgc gacggcgtcg acgagtactt cgagggcgag gccgaacggc
     1501 tcgaatacga cggccagtcg aacaccgtgc gcttcatcag gagcgcccag ctgcgccagc
     1561 tgcgcggcgc gacgacggcc aaccagatca acggcaacct gatcacctac gacgcgacga
     1621 ccgagaagat gacggtctcg ggcggcgccg ccccgaccgc cgcgaacccg ggcggccgcg
     1681 tcagtgcggt gctgacgccg cgcgagggca gcgaggccgc ggccgagatc gccgccgccg
     1741 cgtcggccgc agcatctgct ccgctgaagc tcagccccgc gctgagcgcc ccggcgccgg
     1801 ccgcgaagga caagcgttga gcctgaccgc cggcgccgag atccacccgc gcgagcaccg
     1861 gctcgaagcg agtgggttgc gcaagtccta cggcgcgcgc accgtcgtga aggacgtgca
     1921 cctcgcggtg cgcagcggcg aggtcgtggg cctgctcggc ccgaacggcg ccggcaagac
     1981 caccagcttc tacatgatcg tcggtctggt gcgcgccgac gccggcgaga tcaagatcga
     2041 aggccagcgg gtcgagcggc tgccgatcca ccggcgctcg cgcctgggcc tgtcgtacct
     2101 gccgcaggag gcgtcgatct tccgcaagct gaatgtcgag gagaacatcc gcgccgtgct
     2161 cgagctgcag gtcgacgccg acgacaaggc gctgaagccg gcactgatcg aggaactgct
     2221 cgacggcttg ctgcgcgacc tgtcgatcga caagctgcgc gcctcgccgg cgccgtcgct
     2281 gtcgggcggc gagcgccgcc gcgtcgagat cgcgcgcgcg ctggcgaccc agccgcgctt
     2341 catcctgctc gacgagccgt tcgccggcgt cgacccgatc gcggtgatcg agatccagcg
     2401 catcatcggc ttcctgaaga gccgtggcat cggcgtgctg atcaccgacc acaacgtgcg
     2461 cgagacgctg ggcatctgcg accgcgccta catcatcagc gaaggccgcg tgctcgccga
     2521 gggcacgccg accgagatcg tcgacaacgc cgacgtgcgc aaggtctatc tcggcgaaca
     2581 cttccggatg tgagatggag cccctcgtcc gtcgagtacg ccggccgatc ccccgagggg
     2641 atgcgggccc gcttgggagc ggcccggcgc tgggcccgct cttgcctgag cgagggctga
     2701 tgacatcgtg gccggcatga agcccacgct gcagttccgg ctgtcgcaac acctggcgct
     2761 gacgccgcag ctgcagcagt cgatccggct gctgcaactg tccacgctcg aactccatca
     2821 ggaagtcgag cagatgctcg agagcaaccc cttcctcgag accgacgacg acgccggccc
     2881 ggcgttcgag ccgctcgccg agcgcacgac gccggccgag cgcgtcggcg agcgcgagac
     2941 cgaacgcgcc gccgagaccg gcagcgacgg cgtcggcaac gacgaggcgg cggcgccgct
     3001 ggacgccgcc gagttcggcg gcaccgagcg cgacgactgg gagaacggca ccgagcgcga
     3061 agacttcgac ggcatccgcg agacgcctgg caagagcagc gcgagcccgg acggcgaaga
     3121 cttcgacggc gccgaccgcg acaccgcgat ccccagcctg caggaccatc tgcgcgagca
     3181 gctgtcgagc ctgcgcctgt cggcgagcga cgcggcggcg gtgatggtgc tgatcgagtc
     3241 gctgaacgac gacggctacc tcgccgattc gctcgaggac atcgcgacgc agctcaccgc
     3301 cggcgaggac gaccccgagg ccgaacgcga agcgctgctc gagcggctgc gctgcgcgct
     3361 gcgctacctg cagagcatgg agccgaccgg cgtcggcgcg cgcgacctgg gcgagtgcct
     3421 gaccctgcag ctgcgcaacc ggccgcgcag cgaggcgcag gcgatcgcga tcatcgtctg
     3481 caagcagcac ctcgaactgc tcgcgcgccg cgacctgaag aagctgtgcg cggtgaccgg
     3541 cgccgacgaa gagctgctgc gcgaggcgca ggcgctgatc gtcgcgctcg agcccaagcc
     3601 gggacgccag ttcgcgcgcg ccgaggccaa catcatcatc cccgacgtga tcgtgcagaa
     3661 ggccggccgc ggctggaagg tcacgctgaa ccccgacgtg atgcccaagc tgcgcatcaa
     3721 cgacctgtac gccagcgcga tccgccagca gcgcggcgcc ggctcgcgcg gcgtgcccga
     3781 cgcgctgaac ggcgcgggcg ccgggctgaa ctcgcggctg caggaggcgc gctggttcat
     3841 gaagaacatc ctgcagcgct tcgacacgat ccagcgcgtc tcgcaggcga tcgtcgagcg
     3901 ccagaagaac ttcttcaccc acggcgcgat cgcgatgaag ccgctggtgc tgcgcgagat
     3961 cgccgatgag ctgggcctgc acgaatccac catctcccgc gtgaccaccg ccaagtacat
     4021 gtccacgccc ttcggcacct tcgagttgaa gtacttcttc ggctcgtcc
//
LOCUS       DATMJB010000279         2021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_101158, whole genome shotgun sequence.
ACCESSION   DATMJB010000279 DATMJB010000000
VERSION     DATMJB010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101158"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VNU48_06225"
     CDS             complement(<1..238)
                     /locus_tag="VNU48_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWI10908.1"
                     /translation="MAFPPELSTAAALFLDFDGTLVPIAARPQDVRVPPWVLPTLQAL
                     AARLQGALAIVSGRPIEQLDAFLAPLRLAAAGAHG"
     gene            complement(494..1222)
                     /locus_tag="VNU48_06230"
     CDS             complement(494..1222)
                     /locus_tag="VNU48_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWI10909.1"
                     /translation="MSLALKLMLSPVLIAQAMSTRKRAPALPEAAGPREGQLGHGPGA
                     LRVLIAGDSSAAGVGVRTQDHAFAGHFTRTLHRRSGRPLRWRLRAKSGLTTRELHDLL
                     RADAPPAADIAIVLTGVNDVIELVPPQRAVQHRAALADWLIDEGRARYVLFAPLPPMN
                     QFPLLPQPLRRVMGDDARRHDDAMARWAATRHNVFHTSIAIQLVPGAMADDGFHPGEP
                     VYRTCGEQLARFVAEKILALETRP"
     gene            complement(1235..1990)
                     /locus_tag="VNU48_06235"
     CDS             complement(1235..1990)
                     /locus_tag="VNU48_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWI10910.1"
                     /translation="MTIRTATLNGVATIEIARPEKKNAITGAMYQQMADALDAATADA
                     AVRAVLITGQPGIFTSGNDIDDFMQHPPGESPAFGFMRTLMGCDKPVIAAVTGAAIGI
                     GTTMLLHCDFVYVSDEARLAMPFVSLGLVPEFASSLIVPQLMGNVRAAEKLLLGDPFS
                     GADAVECGIANAVLPAAEVVNHARRIAERFNALPPGAVRESKALMRRARAKLALETVA
                     AEGEVFSKRLHSPEAKEAFSAFLQKRKPDFSKF"
BASE COUNT          286 a          707 c          747 g          281 t
ORIGIN      
        1 cgccgtgcgc gccggcggcg gccagccgca gcggcgcgag aaacgcgtcg agctgctcga
       61 tcggccggcc gctgacgatc gccagcgccc cctgcagccg cgccgccagc gcctgcagcg
      121 tcggcagcac ccagggcggc acgcgcacgt cctgcgggcg cgccgcgatc ggcaccagcg
      181 tgccgtcgaa atcgaggaac aacgccgccg ccgtcgacaa ctcgggggga aaggccagag
      241 tgctcaagcg cgcaccgtag cagactccat cgccgcgccg cgagcgccgg gcgcaaggcg
      301 cgccgtcggc ctacagcgaa actgccggct ggcctacacg aagacgcgcg cttgcccggt
      361 ggcgcaagcc gcgccggtgc ggcagttcag tcgacgaaga agtcgggaat gaagtcggcc
      421 gtgcccggct cgacgctgca gcggtcgagg tcggtggcgc cggtgatgaa cggcgtcttg
      481 cctttcggat cgttcatggg cgtgtctcca gggcaaggat cttctcggcg acgaagcgtg
      541 cgagctgctc gccgcaggtg cggtacaccg gctcgccggg gtggaagccg tcgtcggcca
      601 tcgcgccggg cacgagctgg atcgcgatcg acgtgtgaaa gacgttgtgg cgcgtcgcgg
      661 cccagcgcgc catcgcgtcg tcgtggcggc gcgcgtcgtc gcccatcacg cggcgcagcg
      721 gctgcggcag cagcgggaac tggttcatcg gcggcagcgg tgcgaacagc acgtagcgcg
      781 cccggccctc gtcgatcagc cagtcggcca gcgccgcacg gtgctggacc gcgcgctgcg
      841 gcggcaccag ctcgatcacg tcgttgacgc cggtcagcac gatcgcgatg tcggcggccg
      901 gcggcgcatc ggcgcgcagc aggtcgtgca gctcgcgcgt cgtcagcccc gacttcgcgc
      961 gcagccgcca gcgcaacggg cgaccgctgc ggcgatgcag ggtgcgcgtg aagtggccgg
     1021 cgaacgcatg gtcctgtgtg cggacaccga cgcccgcggc cgacgaatcg cccgcgatca
     1081 gcacgcgcag cgcgccgggg ccgtggccga gctggccctc gcgcgggccg gccgcctcgg
     1141 gcagcgccgg tgcgcgcttg cgcgtgctca tcgcctgcgc gatcagcacc ggcgacagca
     1201 tgagcttgag cgcgagcgac atcacgggat gtgtttagaa cttcgagaag tcgggcttgc
     1261 gcttctgcaa gaacgcgctg aacgcctcct tcgcctcggg gctgtgcaag cgcttgctga
     1321 acacctcgcc ttcggccgcg acggtttcga gcgcgagctt cgcgcgcgcg cggcgcatca
     1381 gcgccttgct ctcgcgcacg gcgcccggcg gcagcgcgtt gaagcgctcg gcgatgcggc
     1441 gggcgtggtt gacgacctcg gcggccggca gcaccgcgtt ggcgatgccg cactcgaccg
     1501 cgtcggcgcc gctgaacggg tcgccgagca gcagcttctc ggccgcacgc acgttgccca
     1561 tcagctgcgg cacgatcagg ctggaggcga actcgggcac cagaccgagg ctgacgaacg
     1621 gcatcgccag acgcgcttcg tccgacacgt agacgaagtc gcagtgcagc agcatcgtgg
     1681 tgccgatgcc gatcgccgcg ccggtcacgg ccgcgatcac cggcttgtcg cagcccatca
     1741 gcgtgcgcat gaacccgaac gccggcgact cgccgggcgg gtgttgcatg aagtcgtcga
     1801 tgtcgttgcc cgaggtgaag atgccgggct ggccggtgat cagcaccgcg cgcaccgcgg
     1861 cgtcggccgt ggccgcgtcg agcgcgtcgg ccatctgctg gtacatcgcg ccggtgatcg
     1921 cgttcttctt ctcggggcgc gcgatctcga tcgtcgcgac gccgttcaag gtggcggtcc
     1981 ggatggtcat ggtgtctcct cgaggtcggt gcgccggcgg a
//
LOCUS       DATMJB010000280         2605 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_487607, whole genome shotgun sequence.
ACCESSION   DATMJB010000280 DATMJB010000000
VERSION     DATMJB010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487607"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..361
                     /locus_tag="VNU48_06240"
     CDS             89..361
                     /locus_tag="VNU48_06240"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008445236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HWI10911.1"
                     /translation="MAKTSSSTATATAPANYEDALGELDRLVGAMEAGQLPLDRLLES
                     YKRGAELLSFCRARLEAVEQQVKVLEDGQLKPLAFAPGPAKPVGTP"
     gene            358..1251
                     /locus_tag="VNU48_06245"
     CDS             358..1251
                     /locus_tag="VNU48_06245"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HWI10912.1"
                     /translation="MKLAEFDAWMRSEADAVESALQAWVPVDAPAGLGLAMRYGVLDG
                     GKRVRPLLVLAAAQAVHGLREAAMRAACAVELIHAYSLVHDDMPCMDNDVLRRGKPTV
                     HVQYGEAQAMLAGDAMQALAFEVLTPDDSAALPAALQARLCSLLARAAGYKGMAGGQA
                     IDLASIGRPLDEHALRDMHHRKTGALLQASVLMGAACGSATPAEWFALAEYGDALGLA
                     FQVVDDILDVTQASATLGKTAGKDLDNNKPTYVTILGLDAARRHATELRDKAQAALAR
                     SGLPDAAWLSLLADKVVERES"
     gene            1254..>2605
                     /gene="dxs"
                     /locus_tag="VNU48_06250"
     CDS             1254..>2605
                     /gene="dxs"
                     /locus_tag="VNU48_06250"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HWI10913.1"
                     /translation="MKHTLLESIASPADLRTLSRAELKQLAAELRDFVVQSVSQTGGH
                     LSSNLGTVELTIALHHVFKTPYDRLVWDVGHQTYPHKILTGRRARMNTLRQLGGISGF
                     PRRDESEYDCFGTAHSSTSISAALGMAVAARLKGEDRKSVAIIGDGAMSAGMAFEAMN
                     NAGVSHADMLVVLNDNDMSISPPVGALNRHLARLMSGKFYATARDTAKTVLKNAPPLF
                     ELAKRFEEHAKGMITPSTLFEEFGFNYTGPIDGHNIDVLIDTLQNVVRLKGPQFLHVV
                     TRKGQGYKLAEADPVLYHGPGKFDPAEGIKKSAAAGKPTYSQVFGDWLCDMARADDRL
                     VAITPAMREGSGLVKFSQEFPERYFDVGIAEQHAVTFAAGLACEGMKPVVAIYSTFLQ
                     RAYDQTIHDVALQNLPVVFAIDRAGVVGADGPTHLGAFDLTYLRCLPNTTVMAPADEN
                     "
BASE COUNT          428 a          901 c          894 g          382 t
ORIGIN      
        1 ggccctgtca cgactcgtca cccctaagtc ggacagtgga tgcccggggc gacccgcctg
       61 actacaatcg aggtttgacg atttggcaat ggcaaagaca agctcctcca ccgcaacggc
      121 caccgcgccc gccaactacg aagacgcgct gggcgagctc gaccgcctgg tcggcgcgat
      181 ggaagcgggc cagctgccgc tcgatcggct gctcgagagc tacaagcgcg gcgccgagct
      241 gctgagcttc tgccgggccc ggctcgaggc ggtcgagcag caggtcaagg tgctcgaaga
      301 cggccagctg aagccgctgg cgttcgcgcc ggggccggcc aagcccgtcg gcacgccatg
      361 aagctggccg agttcgacgc ctggatgcgc agcgaggccg atgccgtcga gtcggcgctg
      421 caggcctggg tgccggtcga cgcgcccgcc ggcctgggcc tggcgatgcg ctacggcgtg
      481 ctcgacggcg gcaagcgtgt gcgcccgctg ctcgtgctgg ccgcggcgca ggcggtgcac
      541 gggctgcgcg aggcggcgat gcgcgcggcc tgcgcggtcg aactgatcca cgcctattcg
      601 ctggtgcacg acgacatgcc gtgcatggac aacgacgtgc tgcggcgcgg caagcccacc
      661 gtgcacgtgc aatacggcga ggcgcaggca atgctcgccg gcgacgcgat gcaggcgctc
      721 gcgttcgagg tgctcacgcc cgacgacagc gccgcgctgc cggccgcgct gcaggcgcgg
      781 ctgtgctcgc tgctggcgcg cgccgccggc tacaaaggca tggccggcgg ccaggcgatc
      841 gatctggcca gcatcggccg gccgctcgac gagcacgcgc tgcgcgacat gcaccaccgc
      901 aagaccggcg ccctgctgca ggccagcgtg ctgatgggtg ccgcctgcgg cagcgcgacg
      961 ccggcggagt ggttcgcgct ggccgagtac ggcgatgcgc tcggcctggc gttccaggtc
     1021 gtcgacgaca ttctcgatgt gacgcaggcc tcggccacgc tgggcaagac cgccggcaag
     1081 gatctcgaca acaacaagcc cacctacgtc accatcctcg gcctcgacgc ggcgcgccgc
     1141 cacgccaccg agctgcgcga caaggcgcag gcggcgctgg cgcgcagcgg cctgcccgac
     1201 gccgcctggt tgagcctgct cgcggacaag gtcgtggagc gcgagagctg atcatgaagc
     1261 acaccctgct cgaatccatt gcgagcccgg cggatctgcg cacgctgtcg cgcgccgagc
     1321 tgaagcagct cgccgccgaa ctgcgtgact tcgtcgtgca gagcgtgtcg cagaccggcg
     1381 gccacctgtc gtcgaacctc ggcaccgtgg agctgacgat cgcgttgcac catgtcttca
     1441 agacgcccta cgaccggctc gtgtgggacg tgggccacca gacctacccg cacaagatcc
     1501 tgaccggccg gcgcgcgcgc atgaacaccc tgcgccagct cggcggcatc tcgggcttcc
     1561 cgcggcgcga cgagagcgag tacgactgct tcggcaccgc gcattcgtcg acctcgatct
     1621 cggctgcgct gggcatggcc gtggcggcgc gcctgaaggg cgaggaccgc aagtcggtgg
     1681 cgatcatcgg cgacggcgcg atgagtgccg gcatggcgtt cgaggcgatg aacaacgccg
     1741 gggtgtcgca cgccgacatg ctggtcgtgc tgaacgacaa cgacatgtcg atctcgccgc
     1801 cggtgggcgc gttgaaccgg catctggcgc ggctgatgag cggcaagttc tacgccaccg
     1861 cccgcgacac cgcgaagacc gtgctgaaga acgcgccacc gctgttcgag ctcgccaagc
     1921 gcttcgagga gcacgccaag ggcatgatca cgccctccac gctcttcgag gagttcggct
     1981 tcaactacac cggccccatc gacggccaca atatcgatgt gctcatcgac acgctgcaga
     2041 acgttgttcg cttgaagggc ccgcagttcc tgcacgtggt gacgcgcaag gggcagggct
     2101 acaagctcgc cgaggccgat cccgtcctct accacgggcc cggcaagttc gatccggccg
     2161 agggcatcaa gaaatccgcc gccgccggca agcccaccta cagccaggtg ttcggcgact
     2221 ggctctgcga catggcgcgc gccgacgatc gcctcgtcgc tatcacgccg gccatgcgcg
     2281 agggctcagg gctggtgaag ttttcgcagg agtttcccga gcgctacttc gacgtgggca
     2341 ttgccgaaca gcatgcggtg acgttcgcgg ccggcctcgc ctgcgagggc atgaagccag
     2401 tggtcgcaat ttattcgacg ttcttgcaac gcgcatacga ccagacgatt cacgacgtcg
     2461 cattgcagaa cttgccggtg gtgtttgcga tcgaccgcgc cggcgtcgtc ggcgccgatg
     2521 gaccgacgca cttgggcgcg ttcgacctca cgtaccttcg ctgcctgccg aacacgacgg
     2581 tgatggcgcc cgccgatgag aacga
//
LOCUS       DATMJB010000281         2481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_559062, whole genome shotgun sequence.
ACCESSION   DATMJB010000281 DATMJB010000000
VERSION     DATMJB010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559062"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /gene="fabZ"
                     /locus_tag="VNU48_06255"
     CDS             <1..381
                     /gene="fabZ"
                     /locus_tag="VNU48_06255"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HWI10914.1"
                     /translation="DRVLELEKGKRILALKNVTMNEPYFVGHFPHRPVMPGVVMLEAL
                     AQAAAILAFDNIGVLPDDKTLFYFAGIDNARFKRPVEPGDQLMLHATLERSRAGIVKF
                     SARALVGDTVAVEAGLMCTMRRLT"
     gene            402..1193
                     /gene="lpxA"
                     /locus_tag="VNU48_06260"
     CDS             402..1193
                     /gene="lpxA"
                     /locus_tag="VNU48_06260"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HWI10915.1"
                     /translation="MAKNIHPTAIVESGADLDSSVTVGAYSIVRAGVRVGAGTSIGPH
                     CVIEGRTTIGRDNRIFQFCSIGAIPQDKKYAGEDTELVIGDRNTIREFCTFNLGVPQA
                     GGITRVGSDNWIMAYTHIAHDCEVGNHTTLANNTTLGGHVQLGDWVTIGGLVGIHQFV
                     KIGAHAMAGFGSAVTQDVPPYMMVDGNPLAVRGFNIEGLRRRGFGPERLAAVKQMHRL
                     LYRDGLSFDAARQAIVALGAETPEAADDIAMMSEFLARVSRGIAR"
     gene            1195..2361
                     /gene="lpxB"
                     /locus_tag="VNU48_06265"
     CDS             1195..2361
                     /gene="lpxB"
                     /locus_tag="VNU48_06265"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HWI10916.1"
                     /translation="MTATDSTAPRFAMVAGEASGDLLGGLLLGGLKARWPQLHAAGIG
                     GPRMVAQGLEAWWPHDKLSVSGYVEVLSHYREISGIRQRLTERLLGADAADRADIFVG
                     IDAPDFNLDLEAKLKAQGMKSIHFVSPSIWAWRGKRIEKIRRATDLVLCLFTFEPAIY
                     AKQGVPAAYVGHPLADVIPLEVPRAGARASLGLADGATVVALLPGSRRAEVQYIAPRL
                     LQAAAAMHRQRPGLRFVLPVVPGLRHVVEPLRRQFAAGVAIDLLDGRSHEALAACDLT
                     LIASGTATLEAALFKRPMVITYVMHALNWQMMKRMNYQPWVGLPNILLNEFAVPEFLQ
                     GDATPAALAQAAFEWLDDPARCDALRLRFEALHRELRCNTAEAATDAIEKVLAG"
     gene            2333..>2481
                     /locus_tag="VNU48_06270"
     CDS             2333..>2481
                     /locus_tag="VNU48_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWI10917.1"
                     /translation="MRSKKFWRAEQLRLVFDGPGLMAGVDEAGRGPLAGPVVAAAVIL
                     DDLLP"
BASE COUNT          375 a          853 c          873 g          380 t
ORIGIN      
        1 gaccgcgtgc tcgagctcga gaagggcaag cgcatcctgg cgctgaagaa cgtgacgatg
       61 aacgagccgt acttcgtcgg ccactttccg caccggccgg tgatgccggg cgtggtcatg
      121 ctcgaggcgc tggcgcaggc cgcggcgatc ctggcgttcg acaacatcgg cgtgctgccc
      181 gacgacaaga cgctgttcta ctttgccggc atcgacaacg cgcgcttcaa gcgcccggtc
      241 gagcccggcg atcagctgat gctgcacgcc acgctcgagc gcagccgcgc cggcatcgtc
      301 aagttctcgg cgcgggccct ggtgggcgac accgtggccg tcgaggccgg gctgatgtgc
      361 acgatgcgcc gcctgacctg agcctctcgc gcgcggcccg catggcgaag aacatccacc
      421 ccacggcgat cgtcgagagc ggcgccgatc tggactcgag cgtcacggtc ggcgcctaca
      481 gcatcgtgcg cgcgggcgtg cgcgtcggcg ccggcacgag catcgggccg cactgcgtga
      541 tcgaagggcg cacgacgatc ggccgcgaca accgcatctt ccagttctgc tcgatcggcg
      601 cgataccgca ggacaagaaa tacgcgggcg aagacaccga gctggtgatc ggcgaccgca
      661 acacgattcg cgagttctgc accttcaacc tcggcgtgcc gcaggccggc ggcatcacgc
      721 gcgtcggcag cgacaactgg atcatggcct acacccacat cgcgcacgat tgcgaggtcg
      781 gtaaccacac cacgctggcg aacaatacga cgcttggcgg tcatgtccag ctcggcgatt
      841 gggtcacgat cggcgggttg gtcggcatcc atcaattcgt caagatcggc gcgcatgcga
      901 tggccggctt cggcagcgcg gtcacgcagg acgtgccgcc ctacatgatg gtcgacggca
      961 acccgctggc ggtgcgcggc ttcaacatcg agggcttgcg gcggcgcgga ttcggccccg
     1021 agcgtctggc cgcggtgaag cagatgcatc gcctgctgta ccgcgacggc ctgagcttcg
     1081 acgcggcgcg ccaggcgatc gtcgcgctcg gcgccgagac gcccgaagcg gcagacgaca
     1141 tcgcgatgat gagcgagttt ctcgctcgcg tttcgcgcgg catcgctcgc tagcatgacc
     1201 gcgacggact ccacagcccc tcgcttcgcg atggtggccg gcgaagcgtc gggcgacctg
     1261 ctcggcggtc tgctgctcgg cggcctgaag gcgcgctggc cgcagctgca tgcggccggc
     1321 atcggcgggc cgcgcatggt tgcgcagggc ttagaggcct ggtggccaca cgacaagctg
     1381 tcggtcagcg gctatgtcga ggtgctgagc cactaccgtg agatctccgg catccgccag
     1441 cggctgaccg agcgcctgct gggcgccgac gccgcggatc gcgccgacat cttcgtcggc
     1501 atcgatgcgc ccgacttcaa tctcgacctc gaggccaagc tcaaggcgca gggcatgaag
     1561 tcgattcact tcgtcagccc gtcgatctgg gcctggcgcg gcaagcgcat cgagaagatc
     1621 cggcgtgcga ccgacctggt gctgtgcctg ttcacgttcg agccggcgat ctacgcgaag
     1681 cagggcgtgc cggccgccta tgtcggtcac ccgctggccg atgtgatccc gctcgaggtg
     1741 ccgcgcgccg gcgcgcgcgc gagcctgggg ctggccgacg gcgcgaccgt cgtcgcgctg
     1801 ctgccgggca gccgccgcgc cgaagtgcag tacatcgcgc cgcgcctgct gcaggcggcc
     1861 gccgcgatgc atcggcagcg cccggggctg cgcttcgtgc tgccggtcgt gccggggctg
     1921 cgccatgtcg tcgagccgct gcgcaggcag ttcgcggccg gcgtcgcgat cgacctgctc
     1981 gacggccgct cccacgaggc gctcgcggcc tgcgacctga ccctgatcgc gagcggcacc
     2041 gcgaccttgg aagcggcgct gttcaagcgg ccgatggtca tcacctacgt gatgcatgca
     2101 ctgaactggc agatgatgaa gcgcatgaac taccagccct gggtcggcct gcccaacatc
     2161 ctgctgaacg agttcgcggt gccggagttc ctgcagggcg atgcgacgcc ggccgcgctc
     2221 gcgcaggccg cgttcgaatg gctggacgac ccggcgcgct gcgacgcgct gcgcctgcga
     2281 ttcgaggcgc tgcaccgcga gttgcgctgc aacaccgccg aggcggccac cgatgcgatc
     2341 gaaaaagttc tggcgggctg agcagctgcg tctcgtcttc gacggccccg ggctgatggc
     2401 cggcgtcgac gaggcgggcc gcggcccgct ggccgggccg gtggtcgcgg ccgcggtgat
     2461 cctggacgat ctgctaccga t
//
LOCUS       DATMJB010000282         3046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_669483, whole genome shotgun sequence.
ACCESSION   DATMJB010000282 DATMJB010000000
VERSION     DATMJB010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669483"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..752)
                     /locus_tag="VNU48_06275"
     CDS             complement(96..752)
                     /locus_tag="VNU48_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HWI10918.1"
                     /translation="MEILQHLLDFILHVDVHLRDFVEAYGAWVYALLFLIIFVETGVV
                     VMPFLPGDSLLFVVGALCGAGLMNLPLVIALLIAAAILGNQSNYTIGRHFGPRVFQWE
                     SSRFFNKAAFDQAHAFYEKYGGVTIVAARFMPFLRTFAPFVAGVAQMTRRRFTMYDVI
                     GGVLWVGGVTLAGYLFGNIPWVKDNLDKIIWGAILLPGALVLLGAWRARRKQQCELKA
                     "
     gene            complement(799..2100)
                     /locus_tag="VNU48_06280"
     CDS             complement(799..2100)
                     /locus_tag="VNU48_06280"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWI10919.1"
                     /translation="MEQSTSTAAAESPAFLRAVTQLAQQRPVLTSRAIYNHNGIKLLE
                     GGVQVDAGLYDRLLSHRLATPLDESVDSDPSVTGAVLREAAEAAMLRWPFFALLGPAG
                     RVRSMLLQSIEAIPLPRPVALHLTLARETRPAVFEHSVLMALLCAHLVREGGAPIHDM
                     TVAASAGLLHDLGMLHIAAEVLDAGNLLSGDARRPLYAHPLTGSMLIARFHEYPSTIA
                     RAILEHHERLDGSGYPRGLAGDAISPLGRLLSLCEVVTAMFDGERKHPELRVSLLLRI
                     GAPRYDPTLVPSIHRLLRALPAPVDAVGASIAESVARLRVLAELLNTWHEVAQAAAPA
                     LVPAGRAVLKSMAEQAAALQRMLFEAGITPEQLDFIGASSEPDAPLRTELWALTGELR
                     WNLRASANQLRRRWRAAMSGQPFPAPIAAWLDAIDALDAPA"
     gene            2234..>3046
                     /gene="mutL"
                     /locus_tag="VNU48_06285"
     CDS             2234..>3046
                     /gene="mutL"
                     /locus_tag="VNU48_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWI10920.1"
                     /translation="MNAVLQPPARRAIRELPDELVSQIAAGEVVERPASVVRELLDNA
                     LDAGATEIAVKLVGGGIRAIVVEDDGSGIPADELALALRRHATSKIASLHELEGVATM
                     GFRGEALAAIAAIAEVSIASRTPQAAHAQRLDARSGELAPAARGVGTSVEVRELFFAT
                     PARRKFLKTEATEFAHCLEAVRRHALARPEVGFTVWHDGKLSAQWRRASGAQRVTDVL
                     GHEFVGQSRELAIDSGVLRVHGRTGTPEAARARADQQYVYVNGRFVRDKLIAH"
BASE COUNT          454 a         1090 c         1100 g          402 t
ORIGIN      
        1 ctcggcacgg ccctgttcca tggcaaggcc gcgccggtcg aggtgttcgc ggtcggcgcg
       61 gcgagcgtct gatcggggta cgtttcgggc ctcgctcagg ccttgagctc gcactgctgc
      121 ttgcgccgcg cccgccacgc gcccagcagc acgagcgccc cgggcagcag gatcgcgccc
      181 cagatgatct tgtcgaggtt gtccttgacc cacgggatgt tgccgaacag gtagccggcc
      241 agcgtcacgc cgccgaccca cagcacgccg ccgatcacgt cgtacatcgt gaagcgccgg
      301 cgcgtcatct gcgcgacgcc ggcgacgaag ggcgcgaagg tgcgcaggaa cggcatgaag
      361 cgcgccgcga cgatggtcac gccgccgtac ttctcgtaga acgcgtgcgc ctggtcgaac
      421 gcggccttgt tgaagaagcg cgaactctcc cactggaaca cgcgcggccc gaagtggcgg
      481 ccgatcgtgt agttgctctg gttgcccagg atcgccgcgg cgatcagcag tgcgatgacc
      541 agcggcaggt tcatcagccc ggcaccgcac agcgcgccga cgacgaacag cagcgagtcg
      601 cccggcagga acggcatcac gacgacgccg gtctcgacga agatgatcag gaacagcagc
      661 gcgtagaccc aggcaccgta ggcctcgacg aagtcgcgca ggtgcacgtc gacgtgcagg
      721 atgaagtcga gcaggtgctg aaggatctcc atgcggcgcg attatcggcg ccgcgcttcg
      781 gccgcccggc cgcggcgttc aggccggcgc gtcgagcgcg tcgatcgcgt ccagccaggc
      841 cgcgatcggc gcggggaacg gctgcccgct catggccgca cgccagcggc ggcgcaactg
      901 gttcgcgctg gcgcgcaggt tccagcgcag ctcgccggtc agcgcccaca actcggtgcg
      961 caggggcgca tcgggctcgc tcgacgcgcc gatgaagtcg agctgctcgg gcgtgatgcc
     1021 ggcctcgaac agcatgcgct gcagcgccgc ggcctgctcg gccatcgact tcagcaccgc
     1081 gcggccggcc ggcaccagcg ccggcgccgc ggcctgcgcg acctcgtgcc atgtattcag
     1141 cagctccgcc agcacgcgca gccgcgcgac cgactcggca atgctcgcgc ccacggcgtc
     1201 cacgggcgcc ggcagtgcgc gcagcaggcg gtggatcgac ggcaccaggg tcgggtcgta
     1261 gcgcggtgcg ccgatgcgca gcagcagcga gaccctcaac tcggggtgct tgcgctcgcc
     1321 gtcgaacatc gcggtgacaa cctcgcacag cgacagcagt cgcccgagcg ggctgatcgc
     1381 gtcgccggcc agaccgcgcg ggtaacccga cccgtcgagg cgttcgtggt gctcgaggat
     1441 cgcgcgcgcg atcgtcgaag ggtattcgtg gaagcgcgcg atcagcatcg agccggtcag
     1501 cgggtgcgca tacaacggcc ggcgcgcgtc gccgctgagg aggttgccgg cgtcgagcac
     1561 ctcggcggcg atgtgcagca tgcccaggtc gtgcagcagc ccggcgctgg ccgccaccgt
     1621 catgtcgtgg atcggcgcgc cgccctcgcg caccaggtgc gcacacagca gcgccatcag
     1681 gacgctgtgc tcgaacaccg cgggccgggt ctcgcgcgcc agcgtcaggt gcagcgcgac
     1741 cggccgcggc agcgggatcg cctcgatcga ttgcagcagc atgctgcgca cgcgtccggc
     1801 cggacccagc agcgcgaaga acggccagcg cagcatcgcc gcttcggcgg cctcgcgcag
     1861 caccgcgccg gtgacgctgg ggtcgctgtc cacgctctcg tcgagcggcg ttgccaggcg
     1921 atgcgagagc agccggtcgt acagcccggc atcgacctgc acgccgcctt cgagcagctt
     1981 gatgccgttg tggttgtaga tcgcacgcga ggtcagcacc gggcgctgct gcgcgagctg
     2041 ggtcaccgcg cgaaggaagg cggggctctc ggcggcggcg gtcgaagtgc tttgttccat
     2101 cggaggtacg gcgtgactgc agccccattg tggacgcggg cggacgtcac ccgatccatc
     2161 gataaggcgg ggcaagcctc gtcaatttcc ccgtcggtgc tgccgccgcg ccggcggttt
     2221 ctacaatgcc ccgatgaacg ccgtcctcca gcccccggcc cgccgcgcca tccgcgagct
     2281 gcccgacgag ctcgtcagcc agatcgccgc cggcgaggtg gtcgaacggc ccgcgtcggt
     2341 ggtgcgcgag ttgctcgaca acgcgctcga cgccggcgcc accgagatcg cggtgaagct
     2401 ggtcggcggc ggcatccgcg cgatcgtcgt cgaggatgac ggcagcggca tcccggccga
     2461 cgagctggcc ctcgcgctgc gtcgccacgc gaccagcaag atcgcctcgc tgcacgagct
     2521 cgagggcgtc gcgacgatgg gctttcgcgg cgaggcgctg gccgcgatcg cggctatcgc
     2581 cgaggtctcg atcgccagcc gcacgccgca ggccgcgcac gcgcagcgcc tggacgcgcg
     2641 ctccggcgag ctcgcgccgg cggcgcgcgg cgtgggcacc agcgtcgagg tgcgcgagct
     2701 gttcttcgcg acgccggcgc ggcgcaagtt cctgaagacc gaggcgaccg agttcgcgca
     2761 ctgcctcgaa gccgtgcggc gccatgcgct ggcgcgcccc gaggtcggct tcacggtctg
     2821 gcacgacggc aagctcagcg cgcagtggcg gcgcgcgagc ggcgcgcagc gcgtgaccga
     2881 cgtgctcggc cacgagttcg tcggccagag ccgcgaactc gcgatcgact ccggcgtgct
     2941 gcgcgtgcac ggccgcaccg gcacgcccga ggcggcacgc gcgcgcgccg accagcagta
     3001 cgtgtatgtc aacggccgct tcgtgcgcga caagctgatc gcgcac
//
LOCUS       DATMJB010000283         3021 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_41900, whole genome shotgun sequence.
ACCESSION   DATMJB010000283 DATMJB010000000
VERSION     DATMJB010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41900"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..1160)
                     /locus_tag="VNU48_06290"
     CDS             complement(279..1160)
                     /locus_tag="VNU48_06290"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HWI10921.1"
                     /translation="MIQRCRFDTTHGLPPDARRAYARDGVIVIEDFMPAARCDALRER
                     ALELVAVHAPAAPGTVFSTRDQRHAQDAYFEASASRIGAFFEAGAFDAAGRLQVPLER
                     AINKLGHALHDLDPVFDAFSRGPRLRAVADAIGLVDAKLVQSMYIFKQPGIGGEVDCH
                     QDSTYLYTEPASVVGFWFAIEDAHRGNGCLGGLPGEHRKGLKEVFRRQRDGSFRLDPC
                     EPAPVWDMSRLKWLEVPKGTLIVFNGSFPHLSETNRSAQSRHAYTLHAVSAAADYPAS
                     NWIQRRAGIDPPFRGFT"
     gene            complement(1157..2266)
                     /gene="queG"
                     /locus_tag="VNU48_06295"
     CDS             complement(1157..2266)
                     /gene="queG"
                     /locus_tag="VNU48_06295"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HWI10922.1"
                     /translation="MDSDRLHALLAQLREHAAQLGFSQIGVADVDLSCAEDGLLRWLD
                     NGFHGAMGYMAAHGLKHARPAELVPGTLRVITARMDYLPQQLANDAHAGWQAIEWRRL
                     AEPAQAALSIYARGRDYHKVLRARLQQLADRLADAIGPFGHRVFTDSAPVLEVELATK
                     SGLGWRGKHTLVLNREAGSMFFLGEIYVDLALPLTAPVEAHCGSCTACIDICPTQAIV
                     APYRLDARRCISYLTIEEPGAIPVELRPLIGNRIYGCDDCQLACPWNKYAQRSVLPDF
                     DVRAAVGNATLLQLWDWSEADFLRHTEGSAIRRIGYERWQRNLAVALGNALRSADDPA
                     LRRALEGRRAASSALVREHIDWALAQALESAPDTR"
     gene            2285..2761
                     /gene="tsaE"
                     /locus_tag="VNU48_06300"
     CDS             2285..2761
                     /gene="tsaE"
                     /locus_tag="VNU48_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWI10923.1"
                     /translation="MPILETRTQLWPDEAACVAAAAALARHRGLAGAFVELHGPLGAG
                     KTTFVRHLLHALGVEGRIKSPTYTVLEPYALVRDGAPLDVSHFDFYRFDDPQEWEDAG
                     FRDVFAAPGLKLAEWPEKAADLLPEADLQIHIEPLAGDARRARFDACSALGRELLR"
BASE COUNT          434 a         1070 c         1071 g          446 t
ORIGIN      
        1 ggcggtgacc gtgacggtct gcagcgtgcc ggccgcggcg ctcgccgccg cggccggcgg
       61 cacggcctgc tgggccatcg cggccggcag cgccagtgcg agcgtcggtc cggccaacag
      121 gccgattcgc aggcgggcgg cgaaagtgtt ctggcggcga gcgcgataac gcatgtgctg
      181 tctcctgctg aggtgcttcg caaggtagca atacatgtgc cacgcgcaaa gccgcgtgaa
      241 gcccgtgtcg catccgcgca ccacggcgcg gatgcggctc aggtgaagcc gcgaaacggc
      301 ggatcgatgc ccgcgcgacg ctggatccag ttgctcgcgg ggtagtcggc ggcggcgctg
      361 accgcgtgca gcgtgtacgc atggcgcgat tgcgccgagc ggttcgtctc cgacaggtgc
      421 ggaaagctgc cgttgaaaac gatcagcgtg cccttcggca cctcgagcca tttgaggcga
      481 ctcatgtccc acaccggcgc gggctcgcac gggtcgagcc tgaagctgcc gtcgcgctgg
      541 cgacggaaca cctctttcaa gcctttgcgg tgttcgcccg gcagcccgcc gaggcagccg
      601 ttgccgcggt gcgcgtcctc gatcgcgaac cagaagccga ccaccgaggc gggctcggtg
      661 tacaggtagg tcgagtcctg gtggcagtcg acctcgccgc cgatgccggg ctgcttgaag
      721 atgtacatcg actgcacgag cttcgcgtcg accaggccga tcgcgtcggc caccgcgcgc
      781 agccgcggcc cgcgcgagaa ggcatcgaac accgggtcga ggtcgtgcag cgcgtggccg
      841 agcttgttga tcgcgcgttc cagcggcacc tgcaggcggc cggcggcgtc gaacgcgccc
      901 gcttcgaaga acgcgccgat gcggctcgcc gaggcctcga agtacgcgtc ctgcgcgtgg
      961 cgctggtcgc gcgtcgagaa caccgtgccg ggcgcggccg gcgcgtgcac cgcgacgagt
     1021 tcgagcgcgc gctcgcgcag cgcgtcgcag cgcgcggcgg gcatgaagtc ctcgatcacg
     1081 atcacgccgt cgcgcgcgta ggcccggcgc gcgtcaggcg gcagcccgtg cgtcgtgtcg
     1141 aagcggcagc gctggatcat cgtgtgtctg gcgcagattc gagcgcctgc gcgagcgccc
     1201 agtcgatgtg ctcgcgcacc agcgcgctgc tggccgcgcg ccggccttcg agcgcgcggc
     1261 gcagcgccgg gtcgtcggcc gagcgcagcg cgttgcccag cgccaccgcg aggttgcgct
     1321 gccaacgctc gtaaccgatg cgccggatcg cgctgccttc ggtgtggcgc aggaagtcgg
     1381 cctcggacca gtcccagagc tgcagcagcg tcgcgttgcc gacggcggcg cgcacatcga
     1441 agtcgggcaa cacgctgcgc tgcgcgtact tgttccacgg gcaggcgagc tggcagtcgt
     1501 cgcagccgta gatgcggttg ccgatcagcg gccgcagctc gacgggaatc gcgcccggct
     1561 cctcgatcgt caggtacgag atgcagcgtc gcgcgtcgag ccggtacggc gcgacgatcg
     1621 cctgcgtcgg acagatgtcg atgcaggccg tgcaactgcc gcaatgggcc tcgaccggtg
     1681 cggtcagcgg cagcgccaga tcgacgtaga tctcgccgag gaagaacatc gagccggcct
     1741 cgcggttcag caccagggtg tgcttgccgc gccagcccag gccgctcttc gtggcgagct
     1801 cgacctcgag caccggcgcc gaatcggtga acacgcggtg gccgaacggg ccgatcgcgt
     1861 cggccagccg gtcggcgagt tgctgcagcc gggcgcgcaa caccttgtgg tagtcgcggc
     1921 cccgcgcgta gatcgacagc gccgcctgcg ccggctcggc caggcgccgc cattcgatcg
     1981 cctgccagcc tgcgtgggcg tcattcgcca attgttgcgg caggtagtcc atgcgtgcgg
     2041 tgatcacgcg cagcgtgccc ggcaccagct cggcgggccg cgcgtgcttc aggccgtgcg
     2101 cggccatgta gcccatcgcg ccgtgaaagc cgttgtcgag ccaccgcaac agcccgtctt
     2161 cggcgcagga caagtccaca tcggcgacgc cgatctgtga aaatccgagc tgcgcggcgt
     2221 gctcgcgcag ctgcgcgagc agcgcgtgaa gccgatccga gtccaaccgc cgagtgccca
     2281 ccatttgccg attctagaaa cccgcacgca actctggccc gacgaggccg cctgcgtcgc
     2341 cgcagccgct gcgctggccc gccatcgcgg cctggccggg gccttcgtcg aactgcacgg
     2401 cccgctgggc gccggcaaga ccaccttcgt gcgccacctg ctgcatgcgc tgggcgtcga
     2461 aggccgcatc aagagcccga cctacaccgt gctcgaaccc tacgcgctgg tgcgcgacgg
     2521 cgcgccgctc gacgtctcgc atttcgattt ctaccgcttc gacgacccgc aggaatggga
     2581 ggacgcgggc tttcgcgacg tcttcgccgc ccccggcctg aagctcgccg agtggcccga
     2641 gaaggccgcc gacctgctgc ccgaagccga cctgcagatc cacatcgagc cgctcgccgg
     2701 cgacgcgcga cgcgcccgct tcgacgcatg cagcgcgctc ggccgcgagc ttctgcgatg
     2761 aagctcgcga cacgcgacgg attcggccac gcggcggctt gcggcccgtt gcggtcgttc
     2821 gcaagcgagc gtgcgccctt tgacggtcgg cgcgagccga gagcgtcggg cggacaactt
     2881 cgctcatgtc ccccggaacg ggctgcgccc gtccctcctc ctttacttcg ctgcgttgcc
     2941 cacccgactc tctcggcagc agaccgaggt gttgtgcgcc ggtcatcggc ctgcacggtg
     3001 gagcagatcg ggccggtggg g
//
LOCUS       DATMJB010000284         3804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_449164, whole genome shotgun sequence.
ACCESSION   DATMJB010000284 DATMJB010000000
VERSION     DATMJB010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3804
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449164"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..544)
                     /locus_tag="VNU48_06305"
     CDS             complement(239..544)
                     /locus_tag="VNU48_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HWI10924.1"
                     /translation="MASLKELIAQKEALEHEIERTRQKDRSDAISKIRTLMDEYGLTS
                     TDLAARKNAKPRGAKGKKVAAKYRDAATGDTWSGRGLQPKWLRAALAGGRKLTDFAV"
     gene            complement(617..892)
                     /locus_tag="VNU48_06310"
     CDS             complement(617..892)
                     /locus_tag="VNU48_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HWI10925.1"
                     /translation="MPRTVQCIHLKKEAEGLDYAPYPGELGKRIYNQVSKEAWQLWMK
                     HQTMLVNENRLNLADQRARQYLARQMENFFFGTGAEQPAGYVPPTST"
     gene            complement(959..2299)
                     /gene="argA"
                     /locus_tag="VNU48_06315"
     CDS             complement(959..2299)
                     /gene="argA"
                     /locus_tag="VNU48_06315"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HWI10926.1"
                     /translation="MSLVFPHTFVPWFRAVAPHIHAYHGKTFVVAITGELIAAGKLNS
                     FAQDLAILHAMGIKLVLVHGFRPQVNEQLRSKGHKARYSNGLRITDAVALDSAQEAAG
                     QLRFEIEAAFSQGLPNTPMANAIVRVVSGNFLTARPVGVVDGVDFIHSGVVRKVDGAA
                     IRRAIDTGAMVLLSPFGFSPTGEAFNLTMEDVATSTAIALQADKLLFITEVPGIHEDL
                     SDPGSAIDTELALAEAERLLATLPRPTQPIDPAFYLQHCVRACRGGVERSHILPFAMD
                     GALLMEVFTHDGIGTMVVDEKLESLREATSDDIGGVLQLIEPFEQDGTLVKRSRTEIE
                     RDIGHYTVIEHDGVIFGCAALYPYPEAKTAEMAALTVSPEVQSQGDGERILKRVEQRA
                     KAAGLESIFVLTTRTMHWFIKRGFVQVDPDWLPEARKRKYNWDRRSQVLVKKIG"
     gene            complement(2409..2726)
                     /locus_tag="VNU48_06320"
                     /pseudo
     CDS             complement(2409..2726)
                     /locus_tag="VNU48_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004566905.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
     gene            2725..3297
                     /locus_tag="VNU48_06325"
     CDS             2725..3297
                     /locus_tag="VNU48_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI10927.1"
                     /translation="MKVRVSPYVQEERLSELQAVSLFRVAQEALRNVERHAQATHIDI
                     RLDDAAASSSCERHPSIRVLMLSMYDNREYVLSAIRAGARAYVLEESPTEEILAAISA
                     VWAGGNYFSAQVSNLVIQPGAAVPQLTAREHEVLLLLAHGRSNKRVARQLDISVCTVE
                     THRLSLRRKLGVDSASELLKIAVTNGWTTL"
     gene            complement(3311..>3804)
                     /locus_tag="VNU48_06330"
     CDS             complement(3311..>3804)
                     /locus_tag="VNU48_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HWI10928.1"
                     /translation="SVYLTARTSAMICWLFVGSALFSAVFGYLGGQSVVENFVKGLGL
                     NAFTFMLLAQAIIFVLGWPLEWTEIIVIFVPIFLPLLQHFQIDPILWGVLVFVNLQAA
                     FLSPPVAMSAFYLKGVSPSHVTLNQIFAGMMPYMLIVILCMVFMYLWPGLTLWLPGYL
                     YSG"
BASE COUNT          632 a         1239 c         1269 g          664 t
ORIGIN      
        1 cgcgcgagcc ggccgacagg ggcaagacag gcacgggcct gggagggcgg gtctcgagca
       61 cgggtggcag cggctgattg tgcgtaaatg taaaggcaaa gcccgcctcg tttcagtgcc
      121 gcatttgcaa agatgaacgc ggcaaggcct tcatctgcgg ggtgtgcgcg gccgcgccgc
      181 ggccatgaaa aaaagccacc ggtcggggtg gctcgtcagc gcttggtcgc gttcggcgtc
      241 agaccgcgaa gtcggtcagc ttgcggccgc ccgccagggc ggccctgagc cacttcggct
      301 gcaggccgcg gccgctccag gtgtcgccgg ttgccgcgtc gcgatatttg gccgccacct
      361 tcttgccctt ggcgccacgc ggtttggcat tcttgcgggc cgcgaggtcg gtgctcgtca
      421 gaccatattc gtccatcagc gtacggattt tcgagatcgc atcgctgcgg tccttttgcc
      481 gggtgcgctc gatctcgtgt tcgagcgctt ccttttgtgc gatcagttcc ttcaacgacg
      541 ccatgctgga ttcctttgtt cagaaaatga atcggtgcga ttccggcatg attctattca
      601 agtcactgaa tcgttttcaa gtactcgtcg gcggcacgta cccggccggc tgctcggcgc
      661 cggtaccgaa gaagaaattc tccatctgcc gcgccagata ttgccgggcg cgctggtcgg
      721 cgagattcag gcgattttcg ttgaccagca tggtctggtg tttcatccac agctgccagg
      781 cttccttgct gacctggttg tagattctct tgccgagctc gcccgggtac ggggcgtaat
      841 ccaggccctc ggcctctttc ttcaaatgga tgcattgaac ggtgcggggc atcttggttt
      901 ctccttcgaa tacgtgaatt cccggcggcg ggatccggct cggcatgaat actgaatttc
      961 agccgatttt cttgaccagt acctgcgagc gccgatccca gttgtacttg cgtttgcgcg
     1021 cctcgggcag ccagtccggg tcgacctgca cgaagccgcg cttgatgaac cagtgcatgg
     1081 tgcgcgtggt cagcacgaag atgctttcca gccccgccgc cttggcgcgc tgctcgacgc
     1141 gcttcaggat gcgctcgccg tcgccctggc tctgcacctc gggcgacacc gtcagcgcgg
     1201 ccatctcggc ggtcttggcc tcggggtacg ggtagagcgc cgcacacccg aagatcacgc
     1261 cgtcgtgttc gatcacggtg tagtggccga tgtcgcgttc gatctcggtg cggctgcgct
     1321 tgaccagcgt gccgtcctgc tcgaacggct cgatcagctg cagcacgccg ccgatgtcgt
     1381 cggacgtggc ctcgcgcagg ctctcgagct tctcgtcgac gaccatcgtg ccgatgccgt
     1441 cgtgcgtgaa cacctccatc agcagcgcgc cgtccatcgc gaacggcagg atgtgcgagc
     1501 gctcgacgcc gccgcgacac gcgcgcacgc aatgctgcag gtagaacgcc gggtcgatcg
     1561 gttgcgtcgg ccgcggcagc gtcgcgagca ggcgctcggc ctcggccagc gcgagctcgg
     1621 tgtcgatcgc gctgccggga tcgctcagat cttcgtgaat ccccggcacc tcggtgatga
     1681 acaacagctt gtcggcctgc agcgcgatcg ccgtgctggt ggccacgtct tccatcgtca
     1741 ggttgaaggc ctcgccggtg ggcgagaaac cgaacggcga cagcagcacc atcgcgccgg
     1801 tgtcgatcgc acgccggatc gccgcgccgt cgaccttgcg cacgacgccg ctgtggatga
     1861 agtcgacgcc gtcgacgacg cccaccggcc gcgcggtcag gaagttgccc gacaccacgc
     1921 gcacgatcgc gttcgccatc ggcgtgttcg gcaggccctg cgagaacgcg gcctcgatct
     1981 cgaagcgcag ctggcccgcg gcctcttgtg ccgagtcgag cgcgaccgcg tcggtgatgc
     2041 gcagcccgtt gctgtagcgc gccttgtgtc ccttggaccg gagctgctcg ttgacctgcg
     2101 gccgaaagcc gtgcaccagc accagcttga tgcccatcgc gtgcaagatg gcgaggtctt
     2161 gcgcgaacga attgagcttg ccggcagcga tcagctcgcc ggtgatcgcg acgacgaagg
     2221 tcttgccgtg gtacgcatgg atgtgcggcg ccacggcacg gaaccagggc acgaaggtgt
     2281 gggggaagac gaggctcacg gcgggttcga ttcactcggg gaacggcgac cgcgattctt
     2341 gcacgtggcg cccgctgccc ggcgccgcat ggggtcgtca gcacggtctc catcaggccg
     2401 gtgaaatccg gcgccggcgc ggcgaagccg ttctcgacgc gccccagctc gcgcagccat
     2461 tgcgcggtgc gcgccaacga cacgcgcacg tgccagctgc cgccctcggt ctgctggcgc
     2521 agcaacgcgg cctgcgcgcc gaacgcgatc aggaagcccg tggtcatgtc gaggatctgc
     2581 accggcagcg ccttcggctg cgcagagtct gccgcctgcg cctcggccag gttgaaaccg
     2641 gttgcgctct gcaccagcga gtcgaggctg cgccgcccgg cccatgggcc ggactcgcca
     2701 taggctgaca gcgacaccgc gacgatgaag gtgcgcgtgt cgccgtacgt gcaggaggag
     2761 cggctgtccg agctgcaggc ggtgagcctg ttccgggtcg ctcaggaggc gctgcgcaat
     2821 gtcgagcggc atgcgcaggc gacccacatc gacattcggc tcgatgacgc ggcggcaagc
     2881 tcgagctgcg agcgccaccc ttcgatccgg gtgctgatgc tgagcatgta cgacaaccgc
     2941 gaatacgtgc tcagcgcgat ccgtgccggt gcccgcgcct acgtgctgga ggagtcgccg
     3001 accgaggaga tcctggcggc gatcagcgcc gtgtgggcgg gcggcaacta cttcagcgcg
     3061 caggtctcga acctggtgat ccagccgggt gccgcggtgc cgcagctcac cgcgcgcgag
     3121 catgaggtcc tgctgctgct cgcgcacggc cgcagcaaca agcgggtcgc gcggcagctc
     3181 gacatcagcg tgtgcaccgt cgagacgcac cggctgagcc tgcgccgcaa gctcggcgtc
     3241 gattcggcca gcgagctgct gaagattgcc gtgacgaacg gctggacgac gctctgaccg
     3301 gcggcgatat tcagccgctg tagaggtatc ccggcagcca cagcgtcagg ccgggccaga
     3361 ggtacatgaa caccatgcac aggatcacga tcagcatgta aggcatcatg ccggcgaaaa
     3421 tctggttcaa ggtcacatgc gatggcgaca cgcccttcag gtagaacgcc gacatcgcca
     3481 ccggcggcga caggaaggcc gcctgcaggt tcacgaacac cagcacgccc cacaggatgg
     3541 ggtcgatctg gaagtgctgc agcagcggca ggaagatcgg cacgaagatc acgatgatct
     3601 ccgtccactc cagcggccag ccgaggacga agatgatcgc ctgcgccagc agcatgaagg
     3661 tgaaggcatt gaggccgagg cccttgacga agttctccac caccgactgg ccgccgaggt
     3721 agccgaacac tgccgagaac agcgccgagc cgacgaacag ccagcagatc atcgcgctgg
     3781 tccgtgccgt caggtagacg cttt
//
LOCUS       DATMJB010000285         7778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_460471, whole genome shotgun sequence.
ACCESSION   DATMJB010000285 DATMJB010000000
VERSION     DATMJB010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7778
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460471"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="VNU48_06335"
     CDS             complement(<1..1369)
                     /locus_tag="VNU48_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI10929.1"
                     /translation="MTTPSQALVWADDLDAPLAHRDGKRLSRRQYLADVRALAARLPA
                     AGPMLNITADRYRFAVGLGAAMLRGQANLMPPNHTPDTVARLRALFPTAYALSDADAP
                     ETALPLVRHAEAPAPADAVAEVPQIDAAALAAQVLTSGSTGAPVPHAKHWGLLVRNTQ
                     AGAQRLAQQMGRRDLVGVTLVATVPSQHMYGFESTVLIALLAGAAFDTGRPFYPADIA
                     AALERAPRPRMLVTTPFHLKTLLDSGVELPAIDLALCATAPLPPQLAVRAEAALAAPL
                     MEIYGCTEAGQVATRRTTAGAEWRTYDGLLLTGDGAETTISGGHVPQPTTLADVLEVL
                     DAQRFRLLGRSNDLINIAGKRSSLSHLNYHLNSIPRVRDGAFWLPDDHAADAAGIVRL
                     AAFVVAPDLPAAAIVAALRERVDTAFLPRPVLQVPALPREPTGKLAATRFAAWAAEQL
                     EAHRRA"
     gene            complement(1366..2034)
                     /locus_tag="VNU48_06340"
     CDS             complement(1366..2034)
                     /locus_tag="VNU48_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10930.1"
                     /translation="MPARRGIRIAAAALMGVVYVLATHWLMTRAPASAWNVVGVLAPM
                     LVAVAIGAWRAGQRWLASGAALLVAALCGQALLGLHSSPQLLYLAQHVGINGFLGVAF
                     GSTLRAGHSPLITTLARRVHREFTPAMVLYTRHCTLAWTLYFVVICVVSVLVYALAPF
                     DVWAVFANLLSPVSVVLMFGAEYLLRYQLHPEFERSSVADSIRSYLHGGAAKAPAAPR
                     DSAA"
     gene            complement(2046..2315)
                     /locus_tag="VNU48_06345"
     CDS             complement(2046..2315)
                     /locus_tag="VNU48_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HWI10931.1"
                     /translation="MTSVQTDLQREVAALVVEALNLDVAPEAIDPQAPLYGEGLGLDS
                     IDILEVALVVSQRYGFQLRSDDEDNVRIFTSLASLTEHIAAHRVA"
     gene            complement(2478..2849)
                     /locus_tag="VNU48_06350"
     CDS             complement(2478..2849)
                     /locus_tag="VNU48_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10932.1"
                     /translation="MDGLTIQALQWARLADLRDVPALDPHDLACMAELREVLLRHGRL
                     RRFALHLVHKHFELGADEVLVEYSDPLAREQWLRVERRDAEVLRSAIPTTWTLADAQP
                     LVACVCAYRAEHGHLGRHQSG"
     gene            complement(2906..6265)
                     /locus_tag="VNU48_06355"
     CDS             complement(2906..6265)
                     /locus_tag="VNU48_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HWI10933.1"
                     /translation="MAAPHIALLNLPAACGPDGTRVVLERKAAALLARLALEGAESRA
                     ALAALLWPDAAPAQARNSLRQRLFRLQHASGCEFVLGADDLHLAEGVTHDLDGARAAL
                     QLDPQAGAGELLGALDYSDSPALAEWVEMARERWRQRRAAWLAEIAAAHEAQHEIARA
                     LAYAQRLLADEPLLEQAHRRVMRLHYLRGDRAAALAAFERCRERLRAELGAAPDRETA
                     ALAKQIAAGDLPALRPNAPLVTVLRPPRLIGRDAQRQRLDSAVRERRSLVLSGEPGIG
                     KSRLLEDLVAAHPALLATGAHAGEAKLPYALLARLAGLAHARFDAPLDSWVREELARL
                     LPAFGAAPAARLDPLRLQQALAAGVAVWAQHGLAGFVVDDLHHADEASLECLLALTEA
                     CGDERLAWIFGVRSLEIPALLSDWLRGADHGAVDTEVVPALDACGVAALLDSLALPQF
                     DAADWAAPLLAHSGGNPLFALETLRAMLALGAGAAPPSGARLPVPAALGALIGRRLAR
                     LSEPALRLLRVAALAGADFDADVAAHVLQAHPLDIVEPWRELEQAQLLAGGRFTHDLI
                     AETAARELPDSIAQALHARLATALEALGRSAARVAPHWARAQAWARAGEVHAIAAGEA
                     QRASRRADEVALWEQAADCFDRAGQPGKAFDARADGVESLIVVRGIPPALVLAGRLAA
                     DARTEPQRMRALTAQATVCLYAGDQAGGEAAARAALETATRLGALWPRFEAARLLAVA
                     LAQAFRAREALEIIEPFRDIVEAEGSLEQRHHFWADYAYALKAAQRLRDTAEALRKAM
                     ASAQALGDHAELATLTSNLAVVVSNFGTPQEALDHGKRARALRDPLGTAVGPAAGAID
                     LYIGVANGALGRYAEALADFDRADACFGTEAPTIWTALTANYRAALLLQLGQFARARQ
                     ALAVEDTAAHGVGARRALLRARIERALGGTGQGALDEALAIVGPQGDHLVRMLARLDA
                     TLTMAPTEAATICAQLGVEADAAEHSAAAMRARLMRLEHLHRGDALDAERGELDAVVA
                     LLDAVEPADMYLPEAWWIVAQACDRLGDARAADAALARGHAWVVERALPNVPAPYRES
                     FLHRNPTNRDLLARAGRRLGLRVPALAASGA"
     gene            complement(6362..6928)
                     /locus_tag="VNU48_06360"
     CDS             complement(6362..6928)
                     /locus_tag="VNU48_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWI10934.1"
                     /translation="MIKTLCIASFALAANAAALAQPAAVLVPAQSEIGFVSTQMGVPV
                     DGHFGRFDAQIALDPKHPETGRVAIDIATASATLGVPETDAELPKPNWFGSAKFPRAS
                     FRSSAIKVLGGGRFEVAGTLTVKGSAQPLVVPVQLTQSAGTSIARGSFTIKRLAFHIG
                     EAEWADTSLVANDVLVRFKLTLTGLAPL"
     gene            complement(6925..7494)
                     /locus_tag="VNU48_06365"
     CDS             complement(6925..7494)
                     /locus_tag="VNU48_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HWI10935.1"
                     /translation="MNTKADANAYGAVAIGLHWLLALAILGSLSVGFYMADLPFSPLR
                     LRLFNWHKWAGITILALSAARLLWRLAHAPPALPADVLAAMPGWQRGAHRATHALLYL
                     LFFAVPLSGWAYTSAAGFPIVWFGVLPLPDWVAVDKELADAVLKPLHHLLAFTLAALV
                     ALHVAATLKHQFVDRDALFWRMWPRGVPR"
     gene            complement(7491..>7778)
                     /locus_tag="VNU48_06370"
     CDS             complement(7491..>7778)
                     /locus_tag="VNU48_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211738.1"
                     /note="catalyzes the formation of
                     3,6-dideoxy-D-glycero-D-glycero-4-hexulose; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HWI10936.1"
                     /translation="YLHEWAQQAAAALPNLRYVPVLSEPKPEDAWAGRTGLVHQAVMH
                     DHPNLMDHQVYACGAPIMVESAQHDFVAKCGLPDDEFYADAFTSEADKHGA"
BASE COUNT         1063 a         2802 c         2941 g          972 t
ORIGIN      
        1 cggcgcgccg gtgcgcttcg agctgctcgg cggcccacgc ggcgaaccgc gttgccgcga
       61 gcttgccggt cggctcgcgc ggcagcgccg gcacctgcag caccgggcgc ggcaggaacg
      121 cagtgtcgac ccgctcgcgc agcgccgcga cgatggccgc cgcgggcagg tcgggcgcga
      181 cgacgaaggc ggccaggcgc acgatgccgg ccgcgtcggc ggcatgatcg tcgggcagcc
      241 agaacgcgcc gtcgcgcacg cgggggatgc tgttcaggtg gtaattcagg tggctcagcg
      301 agctgcgctt gccggcgatg ttgatcagat cgttcgagcg cccgagcagg cggaagcgct
      361 gcgcatcgag cacctccagc acgtcggcca gcgtggtggg ctgcggcacg tggccgccgc
      421 tgatcgtcgt ctcggcgccg tcgccggtca gcagcaggcc gtcgtaggtg cgccattcgg
      481 cgccggcggt cgtgcggcgt gtcgcgacct ggccggcctc ggtgcagcca tagatctcca
      541 tcagcggggc ggccagcgcg gcctcggcgc gcaccgcgag ctggggcggc agcggcgcgg
      601 tcgcgcacaa ggccaggtcg atcgccggca gctccacgcc cgagtcgagc agcgtcttca
      661 ggtggaacgg cgtcgtcacc agcatgcgcg ggcgcggcgc gcgctccagc gcggcggcga
      721 tgtcggcggg gtagaacggc cgcccggtgt cgaacgccgc gccggccagc agcgcgatca
      781 gcacggtcga ctcgaagccg tacatgtgct gcgacggcac ggtcgcgacc agcgtcacgc
      841 cgaccaggtc gcgccggccc atctgctgcg ccaggcgctg cgcgccggcc tgcgtgttgc
      901 gcaccagcag gccccagtgc ttggcgtgcg gcaccggcgc gccggtcgaa cccgaggtca
      961 ggacctgcgc ggcgagcgcg gcggcgtcga tctgcggcac ttcagcgacg gcgtcggcgg
     1021 gcgccggcgc ctcggcatgg cgcaccagcg gcagcgccgt ctcgggggcg tcggcgtcgc
     1081 tcagcgcgta ggccgtcggg aacaacgcgc gcaaccgcgc gaccgtgtcc ggcgtgtggt
     1141 tcggcggcat caggttggcc tggccgcgca gcatcgccgc acccagaccg accgcgaaac
     1201 ggtagcggtc ggcggtgatg ttgagcatcg gcccggccgc gggcaggcgc gccgcgagcg
     1261 cgcgcacgtc ggcgaggtat tggcggcgcg acaggcgctt gccgtcccgg tgcgcgaggg
     1321 gcgcatcgag gtcatcggcc cagaccagcg cttgtgatgg cgtcgtcatg cggcggagtc
     1381 gcgcggcgcg gccggcgcct tcgcggcgcc gccgtgcagg taggagcgga tcgagtcggc
     1441 cacgctgctg cgctcgaact cagggtgcag ctggtagcgc agcaggtact cggcaccgaa
     1501 catcagcacc acgctgaccg gcgacagcag gttcgcgaac accgcccaga catcgaacgg
     1561 ggcaagcgcg tagaccagca ccgagacgac gcagatgacg acgaagtaca gcgtccacgc
     1621 cagcgtgcag tgccgcgtgt acagcaccat cgccggcgtg aactcgcggt gcacgcgccg
     1681 cgccagcgtc gtgatcagcg gcgagtggcc ggcgcgcagc gtgctgccga aggcgacccc
     1741 gaggaagccg ttgatgccca cgtgctgcgc caggtacagc agctgcggcg agctgtgcag
     1801 cccgagcagc gcctggccgc acagcgcggc caccagcagc gcggcgccgc ttgccagcca
     1861 gcgctggccg gcacgccagg cgccgatggc gacggccacc agcatcggcg cgagcacgcc
     1921 gacgacgttc cacgccgacg cgggtgcgcg cgtcatcagc cagtgcgtgg cgagcacgta
     1981 gaccacgccc atcagcgcgg ccgcggcgat gcggatcccc cggcgggcag gcatctcgtg
     2041 ccgccctatg cgacccggtg cgcggcgatg tgctcggtga ggctggccag cgaggtgaag
     2101 atgcgcacgt tgtcctcgtc gtccgaacgc agctggaagc cgtagcgctg cgaaacgacc
     2161 agcgccacct cgaggatgtc gatcgaatcg aggccgaggc cctcgccgta cagcggcgcc
     2221 tgcgggtcga tcgcttcggg agcgacgtcg aggttcaggg cttcgacgac gagtgccgcg
     2281 acttcgcgct gcagatcggt ttggacagag gtcatgctgg tgtgggcccg tttggcatct
     2341 gtcgccgcgc ggggggcgcg gagcggccaa gcgccggaac aatcgaatcg ccggtcgccg
     2401 caaacgttgc gcaccggaag ccccagttta ccaaaggcgc tgcgccgggt cgcccggccc
     2461 cgaccccgcc cgaaccctca gcccgactgg tgccggccga ggtgcccgtg ctcggcacga
     2521 taggcacaga cgcaggccac cagcggctgc gcatcggcca gcgtccaggt cgtcgggatc
     2581 gcgctgcgca gcacctcggc atcgcggcgc tcgacgcgca gccattgctc gcgggcgagc
     2641 ggatcgctgt actcgaccag cacctcgtcg gcgccgagct cgaagtgctt gtgcaccagg
     2701 tgcagcgcga agcgccgcag gcgcccgtgg cgcagcagca cctcgcgcag ttcggccatg
     2761 caggccaggt cgtgcgggtc gagcgccggc acgtcgcgca gatccgcgag ccgcgcccat
     2821 tgcagcgcct gtatcgtcaa gccgtccaag tgctctcccg tgagatcggc gtgctgcacg
     2881 gtaggccgcg cgcgttaccg cagcgttatg cgccgcttgc ggcgagcgcc ggcacgcgca
     2941 gcccgaggcg ccggccggcg cgcgcgagca ggtcgcggtt ggtcgggttg cggtgcagga
     3001 agctttcgcg atacggtgcc ggcacgttcg gcagcgcgcg ctcgacgacc caggcgtggc
     3061 cgcgcgccag cgccgcgtcg gcggcgcgcg cgtcgcccag ccgatcgcag gcctgcgcga
     3121 cgatccacca ggcctcgggc aggtacatgt cggccggctc gaccgcgtcg agcagtgcca
     3181 cgaccgcgtc gagttcgcca cgttcggcgt cgagtgcgtc gccccggtgc aggtgctcga
     3241 gccgcatcag ccgtgcgcgc atcgccgccg cgctgtgctc ggccgcgtcg gcctcgacgc
     3301 cgagctgcgc gcagatcgtg gcggcctcgg tcggggccat cgtcagcgtc gcatcgagcc
     3361 gcgccagcat gcgcaccagg tgatcgccct gcgggccgac gatcgccagc gcctcgtcga
     3421 gcgcgccctg gccggtgcca ccgagcgcgc gctcgatgcg cgcgcgcagc agcgcgcgcc
     3481 gtgcgccgac gccgtgcgcc gccgtgtcct cgacggccag agcctggcgc gcgcgcgcga
     3541 actggcccag ctgcagcagc agcgcggcgc ggtagttggc ggtcagcgcg gtccagatcg
     3601 tcggcgcctc ggtgccgaag caggcgtcgg cgcggtcgaa gtcggccagc gcttcggcgt
     3661 agcggccgag cgcgccgttg gccacgccga tgtacaggtc gatcgcaccg gccgccggcc
     3721 cgaccgcggt acccagcgga tcgcgcagcg cgcgggcgcg cttgccgtgg tcgagcgcct
     3781 cctgcggcgt gccgaagttg ctgaccacga ccgcgaggtt cgaggtcagc gtcgcgagct
     3841 cggcgtggtc gcccagcgcc tgtgcgctcg ccatcgcctt gcgcaaggcc tcggcggtgt
     3901 cgcgcaggcg ctgcgccgcc ttcagcgcgt aggcgtagtc ggcccagaag tgatgccgct
     3961 gctcgaggct gccctcggcc tcgacgatgt cgcgaaaggg ctcgatgatc tcgagcgcct
     4021 cgcgcgcgcg aaaggcctgc gccagcgcga ccgcgagcag ccgcgccgcc tcgaagcgcg
     4081 gccacagcgc gcccagccgc gtcgcggttt cgagcgcggc gcgtgccgcg gcctcgccgc
     4141 cggcctggtc gccggcatac aggcagacgg tcgcctgcgc ggtcagtgcg cgcatgcgct
     4201 gcggctcggt gcgcgcgtcg gcggcgagcc ggccggcgag caccagcgcc ggcgggatgc
     4261 cgcgcaccac gatcagcgat tcgacgccgt cggcgcgcgc gtcgaaggcc ttgccgggct
     4321 ggccggcgcg gtcgaagcag tcggccgcct gctcccacag cgcgacctcg tcggcgcgcc
     4381 gcgatgcgcg ttgcgcctcg cccgcggcga tggcgtggac ctcgccggca cgcgcccacg
     4441 cctgcgcgcg cgcccagtgc ggcgccacgc gcgccgccga gcgcccgagc gcttcgagcg
     4501 cagtggccag gcgcgcatgc agcgcctgcg cgatgctgtc gggcagctcg cgcgccgcgg
     4561 tctcggcgat caggtcgtgg gtgaagcgcc cgcccgcgag cagctgtgcc tgctcgagtt
     4621 cgcgccaggg ctcgacgatg tccagcgggt gcgcctgcag aacgtgcgcg gcgacgtcgg
     4681 catcgaaatc ggcaccggcc agcgcggcga cgcgcagcag ccgcagggcc ggttccgaca
     4741 ggcgcgcgag gcgccgaccg atcaacgcac cgagcgctgc cggcaccggc agccgcgcgc
     4801 ccgacggcgg cgccgcgccc gcgccgagcg cgagcatcgc gcgcagcgtc tcgagcgcga
     4861 acaaggggtt gccgccgctg tgcgcgagca gcggtgcggc ccagtcggcg gcgtcgaact
     4921 gcggcagcgc gagcgagtcg agcagcgcgg ccacgccgca ggcgtcgagg gccggcacga
     4981 cctcggtgtc gaccgcgccg tggtcggcgc cgcgcagcca gtcggacagc agcgccggga
     5041 tctccaggct gcgcacgccg aagatccacg cgagacgctc gtcgccgcag gcttcggtca
     5101 gcgccagcag gcattcgagc gaggcctcgt cggcgtggtg caggtcgtcg acgacgaagc
     5161 cggccaagcc atgctgcgcc cacaccgcga cgccggccgc gagcgcctgc tgcagccgca
     5221 gcgggtccag gcgcgcggcc ggtgcggcgc cgaaggcggg cagcagacgc gcgagttcct
     5281 ccctgaccca gctgtccagc ggtgcatcga agcgcgcgtg cgccaggccg gcgagccgcg
     5341 ccagcagcgc gtacggcagc ttggcctcgc cggcgtgcgc gcccgtggcc agcagcgcgg
     5401 gatgcgccgc gaccaggtcc tcgagcaggc gtgacttgcc gatgccgggc tcgccgctga
     5461 gcaccaggct gcgccgttcg cgtaccgcgc tgtccaggcg ctggcgctgc gcatcgcggc
     5521 cgatcaggcg gggcgggcgc agcacggtga ccagcggcgc gttcgggcgc aacgcgggca
     5581 ggtcgccggc ggcgatctgc ttcgcaagtg cggcggtttc gcggtccggc gcggcgccga
     5641 gttcggcgcg cagccgttcg cggcagcgct cgaacgcggc cagcgccgcc gcacggtcgc
     5701 cgcgcaggta gtgcagccgc atcacgcggc gatgcgcctg ttcgagcagc ggttcgtcgg
     5761 cgagcaggcg ctgcgcatag gccagtgcgc gcgcgatctc gtgctgcgcc tcgtgcgccg
     5821 cggcgatttc ggccagccac gcggcacgcc gctggcgcca gcgctcgcgc gccatctcga
     5881 cccactcggc cagggccggg ctgtcgctgt agtcgagtgc gccgagcagc tcgccggcgc
     5941 cggcctgcgg atcgagttgc agcgccgcgc gcgcgccgtc aaggtcgtgc gtgacgcctt
     6001 cggcaaggtg tagatcgtcg gcgccgagca cgaactcgca accactcgca tgctgcaggc
     6061 ggaacaagcg ctggcgcagg ctgttgcgcg cctgcgccgg cgctgcgtcg ggccacagca
     6121 gcgcggccag cgcggcgcgc gactccgcgc cctcgagcgc gagccgcgcc agcagcgccg
     6181 ccgccttgcg ctccagcacg acgcgcgtgc cgtcgggccc gcatgccgcg ggcaggttca
     6241 gcagcgcgat gtgcggtgcg gccatgtgac aaatcgtacg gcaagttgtg cgcctggcgg
     6301 cgttgaatgc gctggtgcgc ggcgacgagc ttcgtcgtgc agcgcagtcc tggggctgag
     6361 gtcagagcgg cgccaggccg gtgagcgtca gcttgaaacg caccagcacg tcgttggcga
     6421 ccagcgaggt gtcggcccac tcggcctcgc cgatgtggaa ggccaggcgc ttgatcgtga
     6481 agctgccgcg cgcgatcgag gtgcccgcgc tctgcgtcag ctgcaccggc acgaccagcg
     6541 gctgcgcgct gcccttgacc gtgagcgtgc cggccacctc gaagcggccg ccgcccagca
     6601 ccttgatggc gctcgaacga aagctcgccc gcgggaactt cgcgctgccg aaccagttcg
     6661 gcttgggcag ctcggcatcg gtctcgggca cgcccagcgt cgcgctggcg gtggcgatgt
     6721 cgatcgcgac gcggcccgtc tcggggtgtt tcggatcgag cgcgatctgc gcgtcgaagc
     6781 ggccgaagtg cccgtcgacc ggcacgccca tctgggtgct gacgaagccg atctcgctct
     6841 gggccggcac cagcacggcg gcgggttggg cgagcgccgc ggcgttcgct gcgagcgcga
     6901 agcttgcaat gcacagcgtc ttgatcatcg cggaacgcct cgtggccaca tgcgccagaa
     6961 cagcgcgtcg cgatcgacga actggtgctt cagcgtcgcg gccacgtgca gcgcgacgag
     7021 cgcggcgagc gtgaacgcga gcaggtggtg cagcggcttg agcaccgcat cggcgagttc
     7081 cttgtcgacg gccacccagt cgggcagcgg cagcacgccg aaccagacga tcgggaatcc
     7141 cgccgccgag gtgtaggccc agcccgacag cggcaccgcg aagaacagca ggtagagcag
     7201 cgcgtgcgtg gcgcgatgcg caccgcgctg ccagccgggc atcgcggcga gcacgtcggc
     7261 cggcagcgcg ggcggtgcgt gcgcgagccg ccacaacagg cgcgcggccg acaacgcgag
     7321 gatcgtgatg ccggcccact tgtgccagtt gaacaggcgc aggcgcaatg gcgagaacgg
     7381 caggtcggcc atgtagaagc ccacgctgag cgagccgagg atcgccaggg cgagcagcca
     7441 atgcaggccg atggcgacag cgccataagc gttcgcgtcg gccttggtgt tcacgcgccg
     7501 tgcttgtccg cttcggaagt gaacgcgtcc gcatagaact cgtcgtccgg caggccgcac
     7561 ttcgcgacga agtcgtgctg cgccgactcg accatgatgg gcgcgccgca ggcatagacc
     7621 tggtggtcca tcaggttcgg gtggtcgtgc atcacggcct ggtggacgag gccggtgcgg
     7681 ccggcccacg catcttccgg cttcggctcc gacagcaccg ggacgtagcg caggttcggc
     7741 agcgccgcgg ccgcctgctg cgcccactcg tgcaggta
//
LOCUS       DATMJB010000286         4417 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_809273, whole genome shotgun sequence.
ACCESSION   DATMJB010000286 DATMJB010000000
VERSION     DATMJB010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809273"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1187
                     /gene="ychF"
                     /locus_tag="VNU48_06375"
     CDS             96..1187
                     /gene="ychF"
                     /locus_tag="VNU48_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWI10937.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIV
                     ELPDARLQQLATIVKPERIVPAIVEFVDIAGLVAGASKGEGLGNQFLAHIRETDAIVN
                     VVRCFDDENVIHVAGKVDPISDIEVIQTELCLADMGTVEKGLHRYNKVARSGDKEAQK
                     IVDVLTKCMAALDQGKPVRTIAFGKEELLLVKPLCLITAKPAMFVGNVDENGFENNPY
                     LDKLRAYADGQGAPVVAICAKTEAELSEMDDEDKLMFLAEMGQTEPGLNRLIRAAFTL
                     LGLQTYFTAGVKEVRAWTIHVGDTAPQAAGVIHTDFERGFIRAQTIAFADFIAFKGEQ
                     GAKDAGKMRAEGKDYVVKDGDVLNFLFNV"
     gene            1366..>4417
                     /locus_tag="VNU48_06380"
     CDS             1366..>4417
                     /locus_tag="VNU48_06380"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HWI10938.1"
                     /translation="MLTALGYAAAGIAALPLAIPPGYAAPLYPAAGVALASVLIFGRR
                     MLAAIALGALCIGLWLGTQTHGLGGAALPVALAIALGAALQAFVGAALVRRHVQQPLS
                     LTEPRDIAKFLAAAIASCPIGPGIANGALLLAHAVSPADLPFLIGTGFVGNLLGVLIA
                     TPVVLSLLGEPREAWAPRRLSVGVTLALVTLLLAIGIRQLVQWNHERVQAAFTHDATG
                     AAQALSAQLREPLQALEALHGVFMASDYVEPQEMRIASRAWLQSGTLQALGWSQRVAR
                     ADVPAFEARVRAEGLPSYAVFDRPGAVTPPDLAGEVLAMHYIEPSEGNAGALGVNVWS
                     IPPARAAVQAARHSGRPVASAGFRLTQQSADKNEVGVVIYQALYAPRFPSAAQRDASF
                     SGVLFATLRMDAQIAALKAQMPPYLSLCLLDLTPDAPSRRLAGAPDCESRSTELQFTH
                     GFDYAGRRWEVRISSAASDLPGIHSADVWLFALVGLLSTAMLGGFLLIVTGRARRIET
                     AVRDRTAALQTEVRERKATEGLLRESEQRFRNILNNVPIGVIYTDLRGNVKQTNPRFC
                     ELTGYSEDELLGMSLADYTHPEDLLQDIDLMAQLVRGEIPMYRRHERYIARNGDTVWV
                     QATVSLLRDAAAKPRSIVGVVEDITEHLKLEEAERAREAAEASNRAKSEFLSRMSHEL
                     RTPLNAMLGFAQLLELDPRHPLSEAQRPWVSQIQQAGWHLLEMINDVLDLSRIESGNL
                     RLQVETLNLAELIDATVAMVAREAQQRRIAITQEFAPGTATLQGDATRVKQILTNLLS
                     NAVKYNADGGDVHVACRLVGGDAVEIVVTDTGLGMTPEQLAELFQPFNRLGRERSAQQ
                     GTGIGLVISQRLAELMGGSLRARSVAGRGSAFILKLPCSADPDTVRSDLDALAPAPAE
                     YHRRLVHYVEDNETNVEVMRGILAQRPQVELQVSVTGLDGLAAIRARRPDLILLDMHL
                     PDISGMELLRHLKGDRRTAGIPIVIVSADALAHQIDAAFEAGASH"
BASE COUNT          696 a         1560 c         1521 g          640 t
ORIGIN      
        1 caaacagttc ggccacagta tcggcgacgg cgggtcggtc gcctaaaatc cgcccttcgc
       61 ccgcagtttt tgccgggcca tcgagaaaga cccccatgag tttgaaatgc ggcatcgtgg
      121 gcctgcccaa cgtcggcaag tccaccctct tcaatgccct gaccaaggcc ggcatcgcgg
      181 ccgagaacta cccgttctgc acgatcgagc cgaatgtcgg catcgtcgag ctgccggacg
      241 cgcggctgca gcagctggca acaatcgtca agcccgagcg catcgtgccg gcgatcgtcg
      301 agttcgtcga catcgcgggg ctggtcgccg gcgcgtccaa gggcgaaggc ctgggcaacc
      361 agtttctcgc gcacatccgc gagaccgacg cgatcgtcaa cgtggtgcgc tgcttcgacg
      421 acgagaacgt gatccacgtc gccggcaagg tcgatccgat ctccgacatc gaggtgatcc
      481 agaccgagct gtgcctggcc gacatgggca cggtcgagaa gggcctgcac cgctacaaca
      541 aggtcgcgcg ctcgggcgac aaggaagcgc agaagatcgt cgatgtgctg accaagtgca
      601 tggccgcgct cgaccagggc aagccggtgc gcaccatcgc gttcggcaag gaggagctgc
      661 tgctggtgaa gccgctgtgc ctgatcaccg cgaagccggc gatgttcgtc ggcaacgtcg
      721 acgagaacgg cttcgagaac aacccctacc tcgacaagct gcgcgcgtac gccgacggcc
      781 agggcgcgcc ggtggtggcg atctgcgcca agaccgaggc cgagctgtcg gagatggacg
      841 acgaggacaa gctgatgttc ctcgccgaga tgggccagac cgagccgggg ctgaaccggc
      901 tgatccgcgc cgcgttcacg ctgctcgggc tgcagaccta cttcaccgcc ggggtcaagg
      961 aagtgcgggc ctggaccatc cacgtcggcg acaccgcgcc gcaggcggcg ggcgtgatcc
     1021 ataccgactt cgagcgcggc ttcatccgcg cgcagacgat cgccttcgcc gacttcatcg
     1081 ccttcaaggg cgagcagggc gcgaaggacg ccggcaagat gcgcgccgag ggcaaggact
     1141 acgtcgtcaa ggacggcgac gtgctgaact tcctgttcaa cgtttgacgc ggctccgtct
     1201 cgaccttgca acgatttgcc aggccggcgg cccacgccgc gccaagccgc gcaaaatcgg
     1261 ggctctgatg tcccggccac gcgccggccc acccgcagcc cgaacgcccg ccgcttgacc
     1321 accacgaccg cccccggcaa acccctctcc tggacggcag ccttggtgct gaccgccctc
     1381 ggctatgccg cagcgggcat cgcggcgttg ccgttggcga tccctccggg gtatgcggcg
     1441 ccgctctatc cggccgccgg cgtcgcgctg gccagcgtgc tgatcttcgg ccggcgcatg
     1501 ctcgcggcga tcgcgctcgg cgcgctgtgc atcggcctgt ggctgggcac gcagacccat
     1561 ggcctgggcg gcgcggcgct tccggtggcc ctcgcgatcg cgctcggcgc cgcgctgcag
     1621 gccttcgtcg gtgccgcgct ggtcaggcgc catgtgcagc agccgctgtc gctgacggag
     1681 ccgcgcgaca tcgccaagtt cctggcggct gcgatcgcgt cgtgcccgat cgggcccggc
     1741 atcgccaacg gcgcgctgct gctggcgcac gcggtgtcac ccgccgatct gccgttcctg
     1801 atcggcaccg gcttcgtcgg caacctgctg ggcgtgctga tcgccacacc ggtcgtgctc
     1861 agcctgctcg gcgagccgcg cgaggcctgg gcgccgcgac gcctgagcgt gggcgtgacg
     1921 ctggcgctgg tgaccctgct gctggcgatc ggcattcgcc agctcgtgca gtggaaccac
     1981 gagcgcgtcc aggccgcgtt cacacacgat gcgaccggcg cggcgcaggc gttgagcgcg
     2041 cagttgcgcg aaccgctgca ggcgctcgag gcgctgcacg gcgtgttcat ggcctccgat
     2101 tacgtcgagc cgcaggaaat gcgcatcgcg agccgggcgt ggctgcagtc gggcacgctg
     2161 caggcgctgg gctggagcca gcgcgtggcg cgcgccgacg tgcccgcgtt cgaggcccgt
     2221 gtgcgcgccg aagggctgcc ctcgtacgcg gtcttcgatc gacccggcgc ggtgacgccg
     2281 ccggatctcg ccggcgaggt gctggcgatg cactacatcg agccgtccga aggcaacgcc
     2341 ggcgcactcg gggtgaacgt gtggtcgatt ccgccagccc gcgcggccgt gcaagcagcg
     2401 cggcacagcg ggcggcccgt cgccagcgcc ggcttccggc tgacgcagca gagcgcggac
     2461 aagaacgagg tcggcgtcgt catctaccag gcgctgtacg cgccgcgctt cccgagcgcc
     2521 gcgcagcgcg acgcgagctt cagcggcgtg ctgttcgcga ccctgcgcat ggatgcccag
     2581 atcgccgcgc tgaaggcgca gatgccgccc tacctgtcgc tgtgcctgct cgatctgacg
     2641 cccgacgcgc ccagccgccg cctggcgggc gcgccggact gcgagtcgcg cagcaccgag
     2701 ctgcagttca cgcatgggtt cgactacgcc ggtcggcgct gggaggtgcg catttcttcg
     2761 gccgcatcgg acctgccggg catccacagc gccgatgtct ggctgttcgc gctcgtcggg
     2821 ctgctgtcca cggcgatgct cggcggcttc ctgctgatcg tgaccggccg cgcgcggcgc
     2881 atcgagaccg ccgtgcgcga tcgcaccgcg gcactgcaga ccgaggtgcg cgaacgcaag
     2941 gcgacggagg gcctgctgcg cgagagcgaa cagcgcttcc gcaacatcct gaacaacgta
     3001 ccgatcgggg tgatctacac cgacctgcgc ggcaacgtga agcagaccaa cccgcgcttt
     3061 tgcgaactca ccggctacag cgaagacgag ctgctgggca tgagcctggc cgactacacg
     3121 caccccgagg acctgctgca ggacatcgac ctgatggcgc aactggtgcg cggcgagatc
     3181 ccgatgtacc gccggcacga gcgctacatc gcgcgcaacg gcgacaccgt gtgggtccag
     3241 gccacggtct cgctgctgcg cgatgcggcc gccaagccac gcagcatcgt cggcgtggtc
     3301 gaagacatca ccgagcacct gaagctcgaa gaggccgaac gtgcgcgcga ggcggccgag
     3361 gcctcgaacc gggcgaagag cgagttcctg tcgcgcatga gccatgagct gcgcacgccg
     3421 ctgaacgcca tgctgggctt cgcgcagctg ctcgagctcg acccgcggca cccgttgagc
     3481 gaggcgcagc ggccctgggt cagccagatc cagcaggcgg gctggcacct gctggagatg
     3541 atcaacgacg tgctcgacct gtcgcgcatc gaatcgggca acctgcggct gcaggtcgag
     3601 accttgaacc tcgccgagct gatcgacgcc accgtcgcga tggtcgcccg cgaggcgcag
     3661 cagcgccgca tcgcgatcac ccaggagttc gcgcccggca ccgccacctt gcagggcgac
     3721 gcgacccgcg tgaagcagat cctgacgaac ctgctgagca acgcggtcaa gtacaacgcc
     3781 gacggtggcg acgtccacgt cgcgtgtcgg ctggtcggcg gcgatgcggt cgagatcgtc
     3841 gtcaccgaca ccggcctggg catgacgccg gagcagctgg ccgagttgtt ccagcccttc
     3901 aaccggctcg gccgcgaacg ctccgcgcag cagggaaccg gcatcgggct ggtcatcagc
     3961 cagcgcctgg ccgagctgat gggcggctcg ctgcgcgcgc gcagcgtcgc cggccggggc
     4021 tcggccttca tcctgaaact accctgctcg gccgaccccg acacggtgcg ctccgacctc
     4081 gacgcgctcg cgcccgcacc ggcggagtac caccgccggc tggtgcacta cgtcgaggac
     4141 aacgagacca atgtcgaggt gatgcgcggc attctcgcgc agcgcccgca ggtcgagctg
     4201 caggtgtcgg tgaccggcct ggacggcctc gcggcgatcc gcgcgcggcg gcccgacctg
     4261 atcctgctcg acatgcacct gcccgacatc agcggcatgg agctgctgcg gcatctgaag
     4321 ggcgatcggc gcaccgccgg gattccgatc gtgatcgtct cggccgatgc gctcgcccac
     4381 cagatcgatg cggccttcga agcgggcgcg tcgcact
//
LOCUS       DATMJB010000287         3150 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_134866, whole genome shotgun sequence.
ACCESSION   DATMJB010000287 DATMJB010000000
VERSION     DATMJB010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134866"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="VNU48_06385"
     CDS             <1..1436
                     /locus_tag="VNU48_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWI10939.1"
                     /translation="ANGALLWQVKAGDPKAREYFAAAPLVWQGRLYLGVAGSDWGIRG
                     RVMAFDTRDGRELWRFNTIPMGRETGARSWRNPASARTGGGGTWSSYALDPATGELFV
                     SVANPAPDFLPSRRPGDNLFTNSLVVLDARDGRLEWWHQVGRNDSHDWDLAAAPMLYS
                     TRDGQARVALGSKDGHVYAVDRRTRRRVFQTAVTRIENSAAVPTPQGVHVCPGGQAGV
                     HWNGPAFDAARAQLLVGSVDACGTFKSDPAAKRTLGMGYNGGAFTADPQAPLGRVTAL
                     DAATGTTRWRFDSPVPIVAGVTPTAGGLVFTGDLAGDLIALNSDDGRLLGRWASGGAI
                     AGGVISYRVGAAQYVATTSGNISRLSFKSTGSPKIVVFALGDGSPAAAPRVVEVAPAP
                     AGGRADGPVDINWGKLLYASHCAACHGARGEGGGAAPALTGLAQRRSYADTVAFIKDP
                     KAPMPRQFPAPLSAEEVDDIATFIRAF"
     gene            complement(1491..2300)
                     /locus_tag="VNU48_06390"
     CDS             complement(1491..2300)
                     /locus_tag="VNU48_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWI10940.1"
                     /translation="MPFLQYEQAGAVVTLTMNQPETRNALTGNTAVEEFVAAAQRIAT
                     DMSVRVVILTGAGPVFSSGGNVKDMKRFFDDALTPAAIREEYRQGIQRLALAVYNLDV
                     PTIAAVNGPAVGAGCDLACMCDMRIAADTATFAESFIKVGIVPGDGGAWLLPRVVGRS
                     RAAEMSFTGEALSAQEALACGLVSRVVPIAELAAEAGRLAARIAANPGPTLRMTKRLL
                     REGEHMRLESLLELSAGYQALAHKTWQHREAVEAFTEKRKPVFDNPSRGDA"
     gene            complement(2314..>3150)
                     /locus_tag="VNU48_06395"
     CDS             complement(2314..>3150)
                     /locus_tag="VNU48_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI10941.1"
                     /translation="DVFPILEACGRHALPLPLAQTMVARALLSSAGRSAPAGSIALTT
                     SCAANGSAVLVSYGAVADQVLLADGPWLTLLDANTAVREPLDEGSTDAWLTWPADALA
                     KEPFRIESSVDLRSVEACLLAGQMSGAMRQVLRVTLQYANERTQFGRSIGKFQAIQHQ
                     LSVMAEHTEAGRMAARMACATRGIIGSPLLSAVAKARTSEAAALVASIAHAVHGAIGI
                     TEEYDLQLCTRRLHAWRVAAGAESYWNMRIGRALLASRHVTVPEFVRHELSAAPVVAA
                     GT"
BASE COUNT          459 a         1101 c         1132 g          458 t
ORIGIN      
        1 cggcgaacgg cgccctgctg tggcaggtga aggccggcga cccgaaggcg cgcgaatact
       61 tcgcggccgc gccgctggtc tggcagggca ggctctacct cggcgtggcc ggcagcgact
      121 ggggcatccg cggtcgcgtg atggcattcg acacgcgcga cggccgggaa ctgtggcgct
      181 tcaacacgat cccgatgggc cgcgagaccg gcgcgcgcag ctggcgcaac ccggcgtccg
      241 cgcgcaccgg cggcggcggc acgtggagca gctatgcgct cgatccggcg accggggagc
      301 tgttcgtctc ggtcgccaat ccggcgccgg acttcctgcc cagccgtcgt cccggcgaca
      361 acctgttcac caattcgctg gtcgtcctcg atgcccgcga tggccggctc gagtggtggc
      421 atcaggtggg gcgcaacgat tcccacgatt gggaccttgc cgcagcaccc atgctgtaca
      481 gcacgcgcga cggccaggcc cgtgtggccc tgggcagcaa ggacggtcat gtctacgccg
      541 tcgaccggcg cacccgccgg cgtgtcttcc agaccgccgt cacgcgcatc gagaactcgg
      601 ccgccgtgcc gaccccgcag ggcgtgcacg tgtgtcctgg cggccaggcc ggcgttcact
      661 ggaacgggcc ggcgttcgat gcggcgcgcg cgcagctgct ggtcggatcg gtcgacgcgt
      721 gcggcacctt caagtcggac ccggccgcga agcgtacctt gggcatgggc tacaacggcg
      781 gcgcgttcac ggccgatccg caggcgccgc tgggtcgggt cacggcgctc gatgcggcaa
      841 cgggcacgac gcgctggcgc ttcgattcgc cggtgccgat cgtcgccggt gtcacgccca
      901 cggctggagg gctggtcttc acgggtgacc tggcgggcga tctgatcgca ctgaacagcg
      961 acgatggccg ccttctaggg cgctgggcct ccgggggggc catcgccggc ggcgtcatca
     1021 gctatcgcgt gggggcggcc cagtacgtgg ccacgacgtc gggcaacatc tcacggctga
     1081 gtttcaagtc gaccggcagc ccgaagatcg tcgtcttcgc gctcggcgat ggttcgcctg
     1141 ccgcggcacc ccgcgtggtc gaggttgcgc cggcgccggc cggcgggcgc gccgacggcc
     1201 cggtcgacat caactggggc aagctgctgt atgcgagcca ctgcgcggcc tgccacggcg
     1261 cgcgcggcga aggcggcggc gccgcgccgg cactgaccgg tctggcacaa cggcgcagct
     1321 acgccgacac ggtcgccttc atcaaggacc ccaaggcgcc gatgcccagg cagttccccg
     1381 cgccgctgag cgccgaggaa gtggacgaca tcgccacctt catccgggcg ttctaagggg
     1441 atggcggcac gagtccccgt ggacgcgacc acggcaccga gcacgggcca tcacgcgtct
     1501 cctctcgaag gattgtcgaa gacgggcttc ctcttttcgg taaaggcctc gaccgcctcg
     1561 cggtgctgcc atgtcttgtg ggcgagcgcc tggtagccgg cggacagttc aagcagcgac
     1621 tcgagacgca tgtgctcgcc ttcgcgcagg aggcgcttgg tcatgcgcag cgtcggcccg
     1681 ggattggcgg cgatgcgcgc ggcgagcctg ccggcttcgg ccgccagctc ggcgatcggc
     1741 accacccgcg acaccaggcc gcaggcgagc gcttcctggg cgctcaaggc ctcgccggtg
     1801 aacgacatct cggcggcgcg cgaacgaccg acgacgcgcg gcagcaacca ggcgccgccg
     1861 tcgcccggca cgatgccgac cttgatgaag ctctcggcga aggtcgccgt gtcggcagcg
     1921 atccgcatgt cgcacatgca cgccaggtcg cagccggcgc cgaccgccgg gccgttcacg
     1981 gcggcgatcg tcggcacgtc gaggttgtag acggccagcg cgagccgctg gatgccctgg
     2041 cggtattcct cgcgaatggc cgcgggcgtc agcgcatcgt cgaagaaccg cttcatgtcc
     2101 ttgacgttgc cgcccgacga gaagaccggg ccggcgccgg tgagaatgac gacccgcacc
     2161 gacatgtccg tcgcgatgcg ctgcgccgcc gcgacgaact cctcgacggc cgtgttgccg
     2221 gtcagcgcgt tgcgcgtctc gggctggttc atcgtcagcg tgacgacggc accggcctgt
     2281 tcgtactgca gaaatggcat gaaagctcct ggttcaggtg ccggccgcga caacgggcgc
     2341 cgcggacagc tcgtgtctga cgaattcggg cacggtcacg tgccggctgg cgagcagcgc
     2401 gcggccgatg cgcatgttcc agtacgactc ggcgccggcg gcgacgcgcc acgcgtgaag
     2461 gcgccgcgtg cacagctgga ggtcgtattc ctcggtgatg ccgatggcgc cgtgcactgc
     2521 atgggcgatc gacgcgacca gcgctgcggc ttcgctcgta cgggccttgg cgaccgcgct
     2581 cagcagcggc gagccgatga tgccgcgggt tgcgcatgcc atgcgcgccg ccatgcggcc
     2641 cgcctcggtg tgctcggcca tcacgctgag ctgatgctgg atcgcctgga acttgccgat
     2701 cgagcggccg aattgcgtgc gctcgttggc gtactgcagc gtgacgcgca ggacctggcg
     2761 catcgcgccc gacatctggc cggcgagcag gcacgcctcg acgctgcgca gatcgacgct
     2821 cgactcgatc cgaaacggtt ccttggcgag cgcatcggcg ggccaggtga gccaggcatc
     2881 ggtcgagccc tcgtcgagcg gctcgcgcac cgcagtgttg gcgtcgagga gcgtcagcca
     2941 cggcccatcg gccagcagca cctggtcggc caccgcgccg tagctgacca gcacggcact
     3001 tccattcgca gcgcagctcg tggtcagcgc gatcgagccg gcaggcgcgg aacgtccggc
     3061 gctcgacagc agcgcgcggg ccaccatcgt ctgcgcgagc ggcaacggca gcgcatgccg
     3121 gccacaggcc tcgaggatcg gaaagacatc
//
LOCUS       DATMJB010000288         3273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_713234, whole genome shotgun sequence.
ACCESSION   DATMJB010000288 DATMJB010000000
VERSION     DATMJB010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713234"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VNU48_06400"
     CDS             complement(<1..985)
                     /locus_tag="VNU48_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI10942.1"
                     /translation="MTSEEFDLFVIGAGSGGVRAARMAAQRGARVAVADDKPLGGTCV
                     NLGCIPKKLYSFAAHYAEAFEESHGFGWRSGAPVFDWPTLKANRAAEITRLNGIYEQV
                     LTGAGAQVLRGRARVLGAHEVELGGRRYRARNVLVATGGHPVVPPFPGSKLAITSNEI
                     FDLPSFPQHLVVVGGGYIACEFASIFRGLGAQVTQLYRGAQVLRGFDADISDFVADEM
                     RKKGVDLRVRSEVERIERDGGRLRVTLRDGSALVADQLLQATGRDPNTAGLGLAEAGV
                     RLAPNGAVLVDAHYRSNVPGIHAIGDVIDRIHLTPVALAEAMALVEHLFGDG"
     gene            complement(1079..1351)
                     /locus_tag="VNU48_06405"
     CDS             complement(1079..1351)
                     /locus_tag="VNU48_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10943.1"
                     /translation="MSMKKTDLVKNLAKKLDGRMKSAGVPDRFAQGAADATDRREQRR
                     RDAAAGLVPFACKLPGELVKRLHERAAAHDGGVNALVAQALEQALR"
     gene            1441..1995
                     /locus_tag="VNU48_06410"
     CDS             1441..1995
                     /locus_tag="VNU48_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HWI10944.1"
                     /translation="MAIKAIIYKASLQIADMDRSVYVDHAVTIARHPSETDERMMIRL
                     LALALNLPADDHQGTLEFAKDLWDADEPALWHKDLTGEIKHWVEVGQPDDKRLMKASG
                     RADHVSVYSFSASTPIWWAGLATKITRASNIAVWQVDAAQSQALAALAQRGMQLQLSV
                     QDGSAWVGDGQRSVEIAPNRLNPG"
     gene            2042..2317
                     /locus_tag="VNU48_06415"
     CDS             2042..2317
                     /locus_tag="VNU48_06415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI10945.1"
                     /translation="MPLHIEHHPERGRFQAVVEGRTCVADYRLADGVITITHTEVPPP
                     LEGRGIAGELVQALLDHARAQGLKVRPLCSYARAYMRRHPATADLLA"
     gene            2326..>3273
                     /locus_tag="VNU48_06420"
     CDS             2326..>3273
                     /locus_tag="VNU48_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWI10946.1"
                     /translation="MTDTETADASDPFLWLEDVLGERALAWVRERNAQSQALLQARAE
                     FAPIRDGLLEMLNSRERIPQVSRRGDRLYNLWQDEDHPRGVWRRTTLAQYRQTEPAWE
                     TVLDLDALGAAEGENWVWGGAVGLPPAHERVLISLSRGGADAAVVREFDTVAKAFVAG
                     GFALPEAKSEVEWIDADTIYVGTDVGTGSLTSSGYPRTIRRWARGTPLAAATTVFEVA
                     PDDMTAHVAVDATPGHERTLFVRAIDFHRRQHFLLEGAGLRLLDLPEDASIDFWNSAG
                     APADTLLIELRSDWSVGAQTFAGGALLATDAAAFLTGARR"
BASE COUNT          461 a         1206 c         1149 g          457 t
ORIGIN      
        1 tgccgtcgcc gaacagatgc tcgaccagcg ccatcgcctc ggccagcgcc accggcgtca
       61 ggtggatgcg gtcgatcacg tcgccgatcg catggatgcc cggcacattg ctgcgatagt
      121 gcgcgtcgac cagcaccgcg ccgttcggcg cgagccgcac gccggcctcg gccaggccga
      181 gaccggcggt gttcggatcg cggccggtcg cctggagcag ctggtccgcg accagcgcgc
      241 tgccgtcgcg cagcgtcacg cgcagccggc cgccgtcacg ttcgatgcgc tcgacctcgc
      301 tgcgcacgcg caggtccacg cccttcttgc gcatctcgtc ggcgacgaag tcgctgatgt
      361 cggcgtcgaa gccgcgcagc acctgcgcgc cgcggtagag ctgcgtcacc tgcgcgccca
      421 ggccgcggaa gatcgacgcg aactcgcagg cgatgtagcc gccgccgacc accaccaggt
      481 gctgcgggaa gctcggcagg tcgaagatct cgttcgaggt gatcgccagc ttcgagcctg
      541 gaaacggcgg cacgaccggg tgcccgccgg tcgcgaccag gacgttcctg gcgcggtaac
      601 gccggccgcc gagctcgacc tcgtgggcgc cgagcacgcg cgcacggccg cgcagcacct
      661 gcgcgccggc gccggtcaac acctgctcgt agatgccgtt caggcgcgtg atctcggcgg
      721 cgcggttcgc cttcagcgtc ggccagtcga acaccggcgc gccactgcgc cagccgaacc
      781 cgtgcgactc ctcgaaggcc tcggcgtagt gcgccgcgaa gctgtagagc ttcttcggaa
      841 tgcagccgag gttgacgcag gtgccgccca gcggcttgtc gtcggcgacc gcgacgcgcg
      901 cgccgcgttg cgccgccatg cgcgcggcgc gcacgccgcc gctgccggcg ccgatcacga
      961 acaggtcgaa ttcttctgag gtcatgcgaa tgcctccggg cgcatgtttg cgcgacagct
     1021 cttggtcggg ccactgtacg cggcctgaca gaacggcgtg gcccgcgtgc cgtcgcgctc
     1081 agcgcagcgc ctgctccagc gcctgcgcca ccagcgcgtt gacgccgccg tcgtgcgccg
     1141 cggcccgctc gtgcagccgc ttgacgagtt cgcccggcag cttgcaggcg aacggcacca
     1201 gcccggccgc ggcgtcgcgg cgtcgctgct cgcgcctgtc ggtggcgtcg gccgcgccct
     1261 gcgcgaaacg gtccggcacg ccggcgctct tcatgcggcc gtcgagcttc ttcgcgaggt
     1321 tcttgacgag gtcggtcttc ttcatgctca tcgggtgtcc ttcgttcggg gtgtcggcga
     1381 ttgtcccccg cgcgcgctgt ggtccaatcg gccgctcctc acccgcgcgc atcccccgtc
     1441 atggccatca aggcgatcat ctacaaggcc tcgctgcaga tcgccgacat ggaccgcagc
     1501 gtctacgtcg accacgcggt gacgatcgcc cgccatccgt ccgagaccga cgagcgcatg
     1561 atgatccgcc tgctcgcgct ggcgctgaac ctgccggccg acgaccatca gggcacgctc
     1621 gaattcgcga aggacctgtg ggacgccgac gaacccgcgc tgtggcacaa ggacctgacc
     1681 ggcgagatca agcactgggt cgaggtcggc cagccggacg acaagcgcct gatgaaggcc
     1741 agcggccgcg ccgaccacgt cagcgtctac agcttcagcg cgagcacgcc gatctggtgg
     1801 gcggggctgg cgaccaagat cacgcgtgcg tccaacatcg cggtctggca ggtcgatgcc
     1861 gcgcaaagcc aggcgctggc cgcgctggcg cagcgcggca tgcagctgca gctgagcgtg
     1921 caggacggca gcgcctgggt cggcgacggc cagcgctcgg tcgagatcgc gccgaaccgc
     1981 ctgaacccgg ggtgagcgcg gggcgcagcg gcggtcacaa tcgcggctga ccgaatccgc
     2041 catgccgctg cacatcgaac accaccccga acgcggccgc ttccaggccg tcgtcgaggg
     2101 ccggacctgt gtcgccgact accgcctggc cgacggcgtg atcacgatca cccacaccga
     2161 ggtgccgccg ccgctggagg gccgcggcat cgccggcgaa ctggtccagg cgctgctcga
     2221 ccatgcccgc gcgcaggggc tgaaggtgcg cccgctgtgc tcgtacgcgc gcgcctacat
     2281 gcgccgccat cctgccaccg ccgacctgct cgcctgagcc ccaagatgac cgacaccgaa
     2341 acggccgacg cgagcgaccc cttcctgtgg ctcgaggacg tgctcggcga gcgcgcgctg
     2401 gcatgggtgc gcgagcgcaa cgcgcagtcg caggcgctgc tgcaggcgcg cgcggagttc
     2461 gcaccgatcc gcgacggcct gctcgagatg ctgaattcgc gcgagcgcat tccgcaggtc
     2521 tcgcgccgcg gcgatcggct ctacaacctg tggcaggacg aagaccaccc gcgcggggtg
     2581 tggcggcgca cgacgctggc gcaataccgg caaaccgagc cggcctggga gacggtgctc
     2641 gacctcgacg cgctcggcgc cgccgagggc gagaactggg tctggggcgg cgcggtcggg
     2701 ctgccgccgg cgcacgagcg ggtgctgatc tcgctgtcgc gcggcggcgc cgatgcggcg
     2761 gtcgtgcgcg agttcgacac cgtcgcgaaa gccttcgtcg ccggcggctt cgcattgccc
     2821 gaggcgaagt ccgaggtcga gtggatcgac gccgacacga tctacgtcgg caccgatgtc
     2881 ggcaccggct cgctgacgag ctcgggctat ccgcgcacga tccggcgctg ggcgcgcggc
     2941 acgccgctgg cggcggcgac caccgtgttc gaggtcgcgc ccgacgacat gaccgcgcac
     3001 gtcgcggtcg acgcgacgcc gggccacgag cgcacgctgt tcgtgcgcgc gatcgacttt
     3061 caccgccgtc agcacttcct gctggagggc gcggggctgc gcctgctcga cctgcccgag
     3121 gacgcctcga tcgacttctg gaacagtgcc ggcgcacccg ccgacacgct gctgatcgag
     3181 ctgcgcagcg actggtccgt cggcgcgcag acctttgccg gtggcgcgct gctggccacc
     3241 gacgccgcgg ccttcctgac cggcgcgcgg cgc
//
LOCUS       DATMJB010000289         2424 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_416634, whole genome shotgun sequence.
ACCESSION   DATMJB010000289 DATMJB010000000
VERSION     DATMJB010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416634"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VNU48_06425"
     CDS             complement(<1..435)
                     /locus_tag="VNU48_06425"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HWI10947.1"
                     /translation="MVPHLITALNGPINELEQRILESLPAIERWFRLEWMEHTPPFYT
                     SVDVRNAGFKLAPVDTNLFPGGFNNLTDEMLPLAVQAAMAAIEKICPEAKNLLLVPER
                     HTRNTFYLANVQRLTQIFHMAGLNVRLGTLDESITQATELALP"
     gene            complement(674..2194)
                     /locus_tag="VNU48_06430"
     CDS             complement(674..2194)
                     /locus_tag="VNU48_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HWI10948.1"
                     /translation="MRKLLVSLALGFAALGFAGASMAEDAASAPAAAATASAPAAAPA
                     AAPASAAADAASAPAAAASTPVANKGDVAWMIVATLLVIMMTVPGLALFYGGLVRSKN
                     MLSVLMQVMVTFSMIVVLWCIYGYSLAFSEGNAYVGGFDRLFMKGLFNPADGSFAMAA
                     TFSKGVYIPELLFAAFQATFAGITCCLIVGSFVERIKFSAVLLFMALWFTFSYAPIAH
                     MVWFWMGPDAYSGKEVVDAMNGKAGLIWQWGALDFAGGTVVHINAAVAGLVGSYMIGK
                     RVGYGKEAFTPHSLTLTMVGASLLWVGWFGFNAGSALEAGNSAVLAFMNTFSATAAAV
                     LAWCIGESLIKGKASMLGAASGAVAGLVAITPAAGNVGLMGAMVVGAAAGFLCLWGVT
                     GLKKLLGADDSLDVFGVHGVGGITGALLTGIFNNQSLGGPGLVSDWVTATVTSNGIWD
                     QFLIQLKGVCLTVVWSGVVSVVAYFIVDKLIGLRVSEEDEREGLDISAHGETAYHN"
     gene            complement(2218..>2424)
                     /locus_tag="VNU48_06435"
     CDS             complement(2218..>2424)
                     /locus_tag="VNU48_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWI10949.1"
                     /translation="LYRGAEYVVDFLPKVKIEAAVDDAIVDRVIEAIEGAARTGKIGD
                     GKVFVSTLEQVVRIRTGETGKDAL"
BASE COUNT          421 a          826 c          807 g          370 t
ORIGIN      
        1 gggcagcgcg agctcggtgg cctgcgtgat cgattcgtcg agcgtgccca ggcgcacgtt
       61 gagccccgcc atgtggaaga tctgcgtcag ccgctgcacg ttggcgaggt agaacgtgtt
      121 gcgggtgtgg cgctcgggca cgagcagcag gttcttcgcc tcggggcaga tcttctcgat
      181 cgccgccatc gccgcctgca ccgccagcgg cagcatctcg tccgtcaggt tgttgaagcc
      241 gccggggaac aggttggtgt cgaccggcgc gagcttgaag ccggcgttgc gcacgtccac
      301 cgaggtgtag aacggcggcg tgtgctccat ccactcgagc cggaaccagc gctcgatcgc
      361 cggcagcgat tcgaggatgc gctgctcgag ctcgttgatc gggccgttca gggcggtgat
      421 gaggtgcgga accatgagga gcgtctcggc gggcgttgca gcgcaagctg gattctaggc
      481 atctgggggt cccgcacggc cgacgcaagc gccgcgccgg acgaaaaaag gggccgcgca
      541 tgcggcccct ctaccttaca ggggtcgccg taggcgaccc cgctatcgtt tcgggcgcag
      601 cccctcgggg cttgcaagcc ccgaggcttc gccagccaca aggccgcgcg caggcgcgcc
      661 cttgtgcgcg ggctcagttg tgatacgccg tctcgccgtg ggcgctgatg tccaggccct
      721 cgcgctcgtc ttcctcgctc acgcgcaggc cgatgagctt gtcgacgatg aagtaggcaa
      781 ccaccgagac gacacccgac cagacgaccg tcaggcacac gcccttgagc tggatcagga
      841 attggtccca gatgccgttg gacgtgaccg tcgcggtgac ccagtccgac accaggcccg
      901 gaccgccgag cgactggttg ttgaagatgc cggtcagcag cgcgccggtg atgccgccca
      961 caccgtgcac gccgaacacg tcgagcgagt cgtccgcgcc gagcagcttc ttcagaccgg
     1021 tcacgcccca caggcacagg aagcccgcgg cggcgccgac gaccatcgcg cccatcaggc
     1081 cgacgttgcc ggccgccggc gtgatcgcca ccagacccgc caccgcaccc gacgcggcgc
     1141 cgagcatcga ggccttgccc tttatcagcg attcaccgat gcaccaggcg agcacggcgg
     1201 cagccgtggc gctgaaggtg ttcatgaacg ccagcaccgc gctgttgccg gcctcgagcg
     1261 cggagcccgc gttgaagccg aaccagccga cccacagcag cgaggcgccc accatcgtca
     1321 gcgtcagcga gtgcggcgtg aacgcctcct tgccgtagcc gacgcgcttg ccgatcatgt
     1381 aggaaccgac caggcccgcg accgccgcgt tgatgtgcac gaccgtgccg ccggcgaaat
     1441 cgagcgcgcc ccactgccag atcaggccgg ccttgccgtt catcgcgtcg acaacttcct
     1501 tgccgctgta cgcatccggg cccatccaga accagaccat gtgggcgatc ggtgcatagc
     1561 tgaacgtgaa ccacagcgcc atgaacagca gcaccgccga gaacttgatg cgctcgacga
     1621 acgagccgac gatcaggcag caggtgatgc cggcgaacgt ggcctggaac gcggcgaaca
     1681 gcagctcggg aatgtagacg cccttgctga aggtggccgc catcgcaaag gagccgtcgg
     1741 cgggattgaa caggcctttc atgaagaggc gatcgaagcc gccgacgtag gcattgcctt
     1801 cgctgaatgc caggctgtaa ccgtagatgc accacagcac gacgatcatc gagaacgtga
     1861 ccatcacctg catcagcacc gacagcatgt tcttgctgcg caccaggccg ccgtagaaca
     1921 gcgccaggcc cggcaccgtc atcatgatga cgagcagggt ggcgacgatc atccaggcca
     1981 cgtcgccctt gttcgcgacg ggggtcgatg ccgcggcggc gggtgccgac gccgcatcgg
     2041 ccgcggccga tgcgggggcc gccgcaggcg cggccgctgg cgcagacgcc gtcgcggcgg
     2101 cggccggcgc cgacgccgca tcctcggcca tcgaggcgcc ggcaaagccc agcgccgcga
     2161 atcccaaggc caacgaaacg agtagttttc tcatggttgt ctcttttttc ctgtgcgtca
     2221 gagagcgtct ttgccggtct cgccggtgcg gatgcgcacg acctgttcga gcgtggagac
     2281 gaagaccttg ccgtcgccga tcttgccggt gcgcgcagcg ccttcgatcg cttcgatcac
     2341 gcggtcgacg atcgcgtcgt cgaccgcggc ctcgatcttg accttcggca ggaagtcgac
     2401 gacgtactcg gcgccgcggt agag
//
LOCUS       DATMJB010000290         2147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_35783, whole genome shotgun sequence.
ACCESSION   DATMJB010000290 DATMJB010000000
VERSION     DATMJB010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35783"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VNU48_06440"
     CDS             <1..259
                     /locus_tag="VNU48_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006221126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI10950.1"
                     /translation="AVQGYDAAQILGAGLAAVKGDLAKRDALTAAMHKVTVDSPRGKF
                     TISAAGNPVQDIYLREAKGNNNEYRAVAIKALADPARGCKL"
     gene            361..1233
                     /locus_tag="VNU48_06445"
     CDS             361..1233
                     /locus_tag="VNU48_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWI10951.1"
                     /translation="MDLATFLVQCLNAVQYGLLLFLVASGLTLIFGIMGVINLAHGSF
                     YMLGAYLAFVLAPYFGDWFVTQLVVGVLLAAAFGYLLEWAFFSFLYQREHLQQVLMTY
                     ALILVFEEARSLALGNDVHGVQAPAWLAGGFRLGELMSYPWYRVFASVACIVVAIGLY
                     WVVNRTRLGMMIRAGASNRDMARGLGLNIRLLYRVVFAGGVALAALAGMIAAPLSSVY
                     PGMGGSVLIICFVVVVIGGIGSITGALVASLLVGVVDTFGKVFFAEYSGVGVYLLMAV
                     ILVWRPEGLMKRAY"
     gene            1234..>2147
                     /locus_tag="VNU48_06450"
     CDS             1234..>2147
                     /locus_tag="VNU48_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWI10952.1"
                     /translation="MKRAWLVPLVAALVLLALPPVLSPYLHDLVVKIAIYAVFALSLE
                     LLVGVTGLVSLGHAAFLGIGAYVTVLASGDSGGSAARVLPLAVLAAAAYALFVGALSL
                     RTKGVYFIMVTLAFAQMAYFVFHDTKLAGGSDGLFLYVKPTLLGLSLDDKRAMYYTVM
                     LALIGSYALLALLQRSRFGHALAGIRVNEQRTRAAGYPTFRYKLAAFVIAGALAGLAG
                     FLLAVKDGAVNPELLSWHESGAVLLMLILGGIGHLRGAVIGAIAFTLLKELFQLEALL
                     GPLASHWQMTLGLTIIAFVALLPKGLIGL"
BASE COUNT          270 a          718 c          744 g          415 t
ORIGIN      
        1 cgcggtgcag ggctacgacg cggcgcagat cctcggggcc ggcctcgcgg ccgtgaaggg
       61 cgacctcgcc aagcgcgacg cgctgacagc ggcgatgcac aaggtcaccg tcgacagccc
      121 gcgcggcaag ttcacgatct cggcggccgg caacccggtg caggacatct acctgcgcga
      181 ggccaagggc aacaacaacg agtaccgcgc ggtggcgatc aaggcactgg cggacccggc
      241 gcgcggctgt aaattgtgag cttcgagaat cgaacgcttc gctgatttct agcgggcggc
      301 tgcgccgccc ttctctttct ttccggcctg cgcttcgcac ggccgctttg cgccgccgcc
      361 gtggatctcg cgaccttcct cgtccagtgc ctgaacgccg tgcagtacgg cctgctgctg
      421 ttcctcgtgg ccagcggcct gacgctgatc ttcgggatca tgggcgtgat caatctcgcg
      481 cacggcagct tctatatgct cggcgcctac ctcgcattcg tgctcgcgcc gtatttcggc
      541 gactggttcg tcacgcagct cgtcgtcggc gtgctgctcg ccgcggcctt cggctatctg
      601 ctcgaatggg cgttcttcag cttcctgtac cagcgcgaac acctgcagca ggtgctgatg
      661 acctacgcgc tgatcctggt gttcgaggaa gcgcgcagct tggctctggg caacgacgtg
      721 cacggcgtgc aggcaccggc gtggctggcc ggcggctttc ggctcggcga attgatgtcg
      781 tacccgtggt accgcgtgtt cgcgtcggtg gcgtgcatcg tcgtcgccat cggcctctat
      841 tgggtcgtga accgcacgcg gctgggcatg atgatccgcg ccggcgcgag caaccgcgac
      901 atggcgcgcg gcctggggct gaacatccgg ctgctgtacc gcgtcgtgtt cgccggcggc
      961 gtcgcgctgg ccgcgctggc cggcatgatc gcggcgccgc tgtcgtcggt ctacccgggc
     1021 atgggcggca gcgtgctgat catctgcttc gtcgtcgtcg tgatcggcgg catcggctcg
     1081 atcaccggcg cgctggtcgc gtcgctgctg gtgggggtgg tcgacacctt cggcaaggtc
     1141 ttcttcgccg agtacagcgg cgtgggcgtg tacctgctga tggcggtgat cctggtgtgg
     1201 cggcccgaag gcctgatgaa gcgggcctat tgaatgaagc gcgcctggct cgtcccactg
     1261 gtcgcggcgc tggtgctgct ggcgctgccg cctgtgctgt cgccctacct gcacgacctg
     1321 gtcgtgaaga tcgcgatcta cgcggtgttc gcgctcagcc tggagctgct ggtcggcgtg
     1381 accgggctgg tgagcctggg ccacgcggcc ttcctcggca tcggcgccta cgtgaccgtg
     1441 ctggccagcg gcgacagcgg cggctcggcc gcgcgcgtgc tgccgctggc cgtgctcgca
     1501 gccgcggcct acgccctctt cgtcggcgcg ttgagcctgc gcaccaaggg cgtgtacttc
     1561 atcatggtca cgctcgcgtt cgcgcagatg gcctacttcg tgtttcacga caccaagctc
     1621 gccggcggca gcgacggcct gttcctctat gtcaagccga cgctgctcgg gttgagcctg
     1681 gacgacaaac gcgcgatgta ctacacggtg atgctggcgc tgatcggcag ctacgcgctg
     1741 cttgcgctgc tgcagcgttc gcgcttcggc cacgcgctgg ccggcatccg cgtcaacgag
     1801 cagcgcacgc gtgcggccgg ctatccgacc ttccggtaca agctcgccgc cttcgtgatc
     1861 gccggcgcgc tcgccggact ggcgggcttc ctgctcgcgg tgaaggacgg cgcggtcaac
     1921 cccgagctgc tgagctggca cgagtcgggc gcggtgctgc tgatgctgat cctgggcggc
     1981 atcggccacc tgcgcggcgc ggtgatcggc gcgatcgcct tcaccttgct gaaggaactg
     2041 ttccagctcg aggcgctgct gggcccgctc gcgagccatt ggcagatgac gctgggcttg
     2101 acgatcatcg cgttcgtcgc actgctgccg aaaggcctga tcggcct
//
LOCUS       DATMJB010000291         4216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_75475, whole genome shotgun sequence.
ACCESSION   DATMJB010000291 DATMJB010000000
VERSION     DATMJB010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75475"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /gene="carB"
                     /locus_tag="VNU48_06455"
     CDS             <1..863
                     /gene="carB"
                     /locus_tag="VNU48_06455"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857147.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HWI10953.1"
                     /translation="PYSLPTALQDEMRRQTVLMAKALEVVGLMNVQFAIQGEGSDAVV
                     YVLEVNPRASRTVPFVSKATGQPLAKIAARCMVGQKLSDQRNGNGRRPAEVIPPYFSV
                     KEAVFPFNKFPGVDPILGPEMRSTGEVMGAGRTFGEAMLKSQLGAGSKLPTSGTVCIT
                     VKNGDKDRAVKVASELHDLGFNIVATKGTAAAIAAAGVPVKAVHKVKDGRPHIVDMVK
                     GGEIQLVFTTVDETRTAIADSRHIRQAALAHRITYYTSMAGCEAAVEGMKHQDDLVVV
                     SLQDRHRELH"
     gene            938..1414
                     /gene="greA"
                     /locus_tag="VNU48_06460"
     CDS             938..1414
                     /gene="greA"
                     /locus_tag="VNU48_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWI10954.1"
                     /translation="MATIPLTVRGAEKLKEELARLKNVERHAVIMAISEARAQGDLSE
                     NAEYEAAKDKQGFIEGRISEIESKLAAAQIIDPKTLNAEGRVVFGSTVDLEDEDSGAP
                     ATYQIVGDDEADLKQGLISISSPIARALIGKSAGDVAEVQAPGGLRHYEIMAVRYG"
     gene            1414..1869
                     /locus_tag="VNU48_06465"
     CDS             1414..1869
                     /locus_tag="VNU48_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HWI10955.1"
                     /translation="MAGRLAALLAGLWAGVLLCIGAMAAPAAFSMLARPDAGRLVNRL
                     FEQDAYVSIAVAVVLFTIERQRSRGGAAAGTGSVFSANLMLVLGTLFCTVAGYFAVQP
                     MMEAARAGQGAVSFGTLHAVSAGFFVLKGLLVLALAWRLTGISRTVTTS"
     gene            complement(1844..2320)
                     /locus_tag="VNU48_06470"
     CDS             complement(1844..2320)
                     /locus_tag="VNU48_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HWI10956.1"
                     /translation="MTAIQLTPAQRKDHRAAAHHLAPVVMIGGDGLTPAVQKEVDAAL
                     NAHGLIKVRVFCDERATRETIFTTLADALGAAPIQHIGKLLVLWRPMPPKEKVEREGR
                     GAGPRVVKILKFSKSGNHRAQVKKITVMGNMRIAAGGELKRATKRRTSVKKSSQSD"
     gene            2341..3051
                     /locus_tag="VNU48_06475"
     CDS             2341..3051
                     /locus_tag="VNU48_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HWI10957.1"
                     /translation="MKTQTKSKKVNKAWLHDHMNDPYVKQAQREGYRARAAYKLKEID
                     EELHLLKPGQIVVDLGSVPGAWSQYVRRKFAPKEAGVGGAAAGALNGAIIALDVLAME
                     PIEGVTFLQGDIREESVLAQLEDIVAGRPVDVVVSDMAPNLSGIESADSARISHLVEL
                     AVEFAQKHLTPNGALVCKVFHGSGHSQLVKLFKTNFRVVKPIKPKASRDKSAETFLVG
                     IGLKSPVQGRAGGVDNPT"
     gene            3209..>4216
                     /locus_tag="VNU48_06480"
     CDS             3209..>4216
                     /locus_tag="VNU48_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HWI10958.1"
                     /translation="MNNQWFSKVAVWLVIALVLFTVFKQFDRTAQQSGMMGYSDFLEE
                     VRAKHIKSVQLQDVGGGSTEIRARTNDDKEVRTTATALDRGLVGDLINNNVKFDVKQR
                     EEPSFLVGILMSWGPILLLIGVWIYFMRQMQGGGKGGAFSFGKSKARMLDEANNTTTF
                     ADVAGCDEAKEEVKELVDFLKDPQKFQKLGGRIPRGVLLVGPPGTGKTLLAKAIAGEA
                     KVPFFSISGSDFVEMFVGVGAARVRDMFEQAKKSAPCIIFVDEIDAVGRHRGAGLGGG
                     NDEREQTLNQMLVEMDGFETNLGVIVMAATNRPDILDPALLRPGRFDRQVYVTLPDIR
                     GR"
BASE COUNT          717 a         1381 c         1450 g          668 t
ORIGIN      
        1 caccgtactc gctgccgacc gcgctgcagg acgagatgcg gcgccagacg gtgctgatgg
       61 ccaaggcgct cgaggtcgtc ggactgatga acgtgcagtt cgcgatccag ggcgagggca
      121 gcgacgcggt cgtctacgtg ctcgaggtca acccgcgcgc gtcgcgcacc gtgcccttcg
      181 tctcgaaggc gaccggccag ccgctggcga agatcgcggc gcgctgcatg gtcgggcaga
      241 aattgtcgga tcagagaaac ggcaatgggc gtcggccggc cgaggtgatc ccgccgtact
      301 tctcggtcaa ggaagcggtc ttcccgttca acaagttccc cggcgtcgac ccgatcctcg
      361 ggcccgagat gcgctcgacc ggcgaagtga tgggggcagg ccgcacgttc ggcgaggcga
      421 tgctgaagag ccagctcggc gccggctcga agctgccgac cagcggcacg gtctgcatca
      481 ccgtgaagaa cggcgacaag gatcgcgcgg tgaaggtcgc cagcgagctg cacgacctgg
      541 gcttcaacat cgtcgccacc aagggcacgg ccgcggcgat cgcagccgcc ggcgtgccgg
      601 tgaaggcggt ccacaaggtc aaggacggcc ggccgcacat cgtcgacatg gtcaagggcg
      661 gcgagatcca gctcgtcttc acgacagtgg atgaaacccg caccgcgatc gccgattcgc
      721 gccacatccg tcaggcggcg ctggcgcatc gcatcaccta ctacaccagc atggcgggct
      781 gcgaggcggc ggtcgagggc atgaagcacc aggacgacct ggtcgtggtg tctctgcagg
      841 accggcaccg cgaactgcac taaactccgg cccattcagt cccgccgccg gccaaccccg
      901 gcggcggcgt tatttttgcg atcaaggttt gttcatcatg gccaccattc ccctcacggt
      961 ccgcggtgcc gaaaagctca aggaagagct cgcgcggctg aagaacgtcg aacgccacgc
     1021 ggtgatcatg gccatctcgg aggcgcgcgc gcagggcgac ctgtcggaga acgccgagta
     1081 cgaggccgcc aaggacaagc agggcttcat cgagggccgc atctcggaga tcgaaagcaa
     1141 gctcgcggcg gcgcagatca tcgacccgaa gacactgaac gccgagggac gcgtcgtgtt
     1201 cggctcgacc gtcgatctgg aagacgaaga cagcggcgcc ccggcgacct accagatcgt
     1261 cggtgacgac gaggccgacc tgaagcaggg cctgatctcg atcagctcgc cgatcgcgcg
     1321 cgcgctgatc ggcaagtcgg ccggcgacgt ggccgaggtg caggcgccgg gcgggctgcg
     1381 gcactacgag atcatggccg tgcgttacgg ctgatggctg gtcgtctcgc cgcgctgctg
     1441 gccggcctgt gggccggcgt gctgctgtgc atcggcgcga tggcggcacc ggccgccttt
     1501 tcgatgctgg cgcgcccgga cgcgggccgc ctcgtcaacc gactgttcga gcaggacgcc
     1561 tatgtgagca tcgcggtcgc ggtcgtcctg ttcacgatcg aacgccagcg cagccggggc
     1621 ggcgccgcgg ccggtaccgg ctcggtgttc agcgccaacc tgatgctcgt gctcggcacc
     1681 ctgttctgca ccgtggccgg ctacttcgcc gtccagccga tgatggaagc ggcgcgcgcc
     1741 gggcagggcg ccgtctcgtt cggcacgctg cacgcggtct cggccggctt cttcgtgctg
     1801 aagggcctgc tcgtgctggc gctggcgtgg cgcctgaccg ggatcagtcg gactgtgacg
     1861 acttcttgac gctggtgcgg cgcttggtcg cccgcttcag ctcgccgccg gcggcgatgc
     1921 gcatgttgcc catcaccgtg atcttcttga cctgcgcgcg gtggttgccg ctcttcgaga
     1981 acttcaggat cttgacgacc cgcggcccgg cgccgcggcc ctcgcgttcg accttttcct
     2041 tcggcggcat cgggcgccac agcaccagca gcttgccgat gtgctggatc ggcgcggcac
     2101 cgagcgcgtc ggccagcgtc gtgaagatcg tctcgcgcgt ggcgcgctcg tcgcagaaca
     2161 cgcgcacctt gatcaggccg tgggcgttca gcgccgcgtc cacttccttc tgcacggcag
     2221 gcgtcaggcc gtcgccgccg atcatcacca ccggggccag gtgatgggcg gcggcgcggt
     2281 ggtccttgcg ctgggcggga gtgagttgga tcgcggtcat cctcgtatta tccctgcgct
     2341 atgaaaaccc aaaccaaaag caagaaggtc aacaaggcgt ggttgcacga ccacatgaac
     2401 gacccgtacg tgaagcaggc gcagcgcgag ggctaccgcg cccgcgccgc ctacaagctg
     2461 aaggagatcg acgaggaact gcatctgctc aagcccggcc agatcgtcgt cgacctggga
     2521 tcggtgcccg gcgcctggag ccagtacgtg cggcgcaagt tcgcgccgaa ggaagccggc
     2581 gtcggcggtg ccgcggcggg cgcgttgaac ggcgccatca tcgcgctgga cgtgctggcg
     2641 atggagccga tcgagggcgt gaccttcctg cagggcgaca ttcgcgagga gtcggtgctg
     2701 gcgcagctcg aggacatcgt cgccgggcgc ccggtcgacg tggtcgtttc cgacatggcg
     2761 ccgaacctgt cgggcatcga gagcgccgat tcggcgcgca tcagccacct ggtcgagctc
     2821 gccgtcgaat tcgcccagaa gcacctgacg ccgaatggcg cgctggtctg caaggtgttc
     2881 catggcagcg gccacagcca gctcgtgaag ctgttcaaga cgaacttccg ggtcgtcaag
     2941 ccgatcaagc ccaaggcctc gcgcgacaag tcggccgaga cctttctggt cgggatcggg
     3001 ttgaaatcgc ccgttcaagg ccgcgccggc ggggtcgata acccgacatg aggccgtgcc
     3061 agcgtgtcgg acagctcgat acacgcccgc ccgccggggg attgcgtgtc ggcgccgggg
     3121 cttgactgga ctaaaatcgg ccgcagatgc ccgggatggc ggcgcttggc gccctgcctt
     3181 cctgtcatga cccgaaactg gagtcgcggt gaacaatcaa tggttctcta aggttgctgt
     3241 ctggctggtg atcgcactcg tgctgttcac cgtgttcaag cagttcgacc gaacggccca
     3301 gcagtcgggg atgatgggct actccgattt cctcgaagag gtccgggcca agcacatcaa
     3361 gagcgtgcag ttgcaggacg tcggcggcgg cagcaccgag atccgcgccc gcaccaacga
     3421 cgacaaggaa gtgcgcacca ccgccactgc gctcgaccgc ggcctcgtcg gcgacctgat
     3481 caacaacaac gtcaaattcg atgtcaaaca gcgcgaggag ccctcgttcc tggtcggcat
     3541 cctgatgtcc tggggcccga tcctgctgct gatcggcgtc tggatctatt tcatgcgcca
     3601 gatgcagggc ggcggcaagg gcggcgcgtt cagcttcggc aagagcaagg cgcgcatgct
     3661 cgacgaggcc aacaacacga ccaccttcgc cgacgtcgcc ggctgcgacg aggccaagga
     3721 agaggtcaag gagctggtcg acttcctgaa agatccgcag aaattccaga agctcggcgg
     3781 gcgcatcccg cgcggggtgc tgctggtcgg cccgccgggg accggcaaga cgctgctcgc
     3841 gaaggccatc gccggcgaag cgaaggtgcc gttcttcagc atcagcggct ccgacttcgt
     3901 cgagatgttc gtcggcgtcg gcgcggcgcg cgtgcgcgac atgttcgagc aggccaagaa
     3961 gagcgcaccg tgcatcatct tcgtcgacga gatcgacgcg gtcggccgcc atcgcggcgc
     4021 cggcctgggc ggcggcaacg acgagcgcga gcagacgctg aaccagatgc tggtcgagat
     4081 ggacggcttc gaaacgaatc tcggcgtgat cgtgatggcc gcgacgaacc gtccggacat
     4141 cctcgatccc gcgctgctgc gtccgggccg cttcgaccgc caggtctacg tcacgctgcc
     4201 cgatatccgc ggccgc
//
LOCUS       DATMJB010000292         2238 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_195448, whole genome shotgun sequence.
ACCESSION   DATMJB010000292 DATMJB010000000
VERSION     DATMJB010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195448"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="bktB"
                     /locus_tag="VNU48_06485"
     CDS             <1..1000
                     /gene="bktB"
                     /locus_tag="VNU48_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketothiolase BktB"
                     /protein_id="HWI10959.1"
                     /translation="DAYLSRVAALDAGLPQETPAFNVNRLCGSGLQAVVSAAQALLLG
                     DAEIAIGAGAESMSRGPYLMTAGRWGIRMGDSQLLDGMTGILHDPWLKLHMGVTAENV
                     AARYGITRAMQDELALQSQQRAARAIAEGRFTAQIVPVELPARKGQAASFDTDEHVRG
                     TLTLEQLAAMKPAFKKDGGTVTPGNASGINDGAAAVLLAEAAAAQAQGLTPLARLVAY
                     AHVGVEPELMGIGPVPATRLALQRAGLRAHQMDVIEANEAFAAQACAVMRELDLDPAK
                     VNPNGSGISLGHPVGATGALITVKALHELQRVGGRYALVTMCIGGGQGIAAIFERV"
     gene            1013..2179
                     /locus_tag="VNU48_06490"
     CDS             1013..2179
                     /locus_tag="VNU48_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI10960.1"
                     /translation="MDFDIPAPLRELQQRTRRFIADEVIPLENDARCTPHGPGEPLRA
                     ELIARAAAAGLLAPHVGRDFGGLGLDHRGKAIVFEEAGYSPLGPVALNIMAPDEGNMH
                     LLEAVAGPAQQERWLRPLAAGRTRSCFCMTEPDPGAGSDPGMLQTRAVRDGDDYLISG
                     RKWFITGADGAAFAIIMARLEDGRATMFLADMDRPGITVERSMDTLDSCFPGGHGVVR
                     FDELRVPAADVLGAPGEGFKYAQVRLSPARLTHCMRWLGAARRAHDTAVAYARRRHAF
                     GKPLAEHEGVGFGLADNEMDLHLTRLSVWHTAWVLDQGHLGTTESSMCKVIASEAIWR
                     VVDRSVQFLGGQGVTGETIVARIFADVRAFRIYDGPSEVHRWSLARRIFKGTVT"
BASE COUNT          325 a          827 c          786 g          300 t
ORIGIN      
        1 agacgcatat ctctccagag tcgcggcgct cgacgccggg ctgcctcagg agacccctgc
       61 gttcaacgtc aaccgcctct gcggctccgg actgcaggcg gtcgtctccg cggcgcaggc
      121 gctcttgctc ggcgacgccg agatcgcgat cggcgccggc gccgaatcga tgagccgcgg
      181 cccctacctg atgacggccg gccgctgggg catccgcatg ggcgactcgc agttgctcga
      241 cggcatgacc ggcatcctgc acgacccgtg gctgaaactc cacatgggtg tcaccgccga
      301 gaacgtcgcc gcacgctacg gcatcacgcg cgccatgcag gacgagctgg cgctgcagag
      361 ccagcagcgc gccgcacgcg ccatcgccga ggggcgcttc accgcgcaga tcgttcccgt
      421 cgaactgccg gcccgcaagg ggcaggcagc gagcttcgac accgacgagc acgtgcgcgg
      481 cacgctgacg ctcgaacagc tcgccgcgat gaagccggcc ttcaagaagg acggcggaac
      541 ggtcacgccg ggcaacgcct cgggcatcaa cgacggcgcc gccgcggtgc tgctggccga
      601 agccgcggca gcgcaggctc agggcctgac accgctggcc cgcctggtgg cctacgccca
      661 tgtcggcgtc gagcccgagc tcatgggcat cggcccggtg ccggccaccc gcctggcgct
      721 gcagcgcgcc gggctgcgcg cgcaccagat ggacgtgatc gaggccaatg aggcatttgc
      781 cgcccaggcc tgtgcggtga tgcgcgagct cgatcttgac ccggccaagg tcaacccgaa
      841 cggctcgggc atctcgctcg gccacccggt gggcgccacc ggcgcgctca tcaccgtcaa
      901 ggcactgcac gagttgcagc gcgtcggcgg ccgctatgca ctggtgacga tgtgcatcgg
      961 cggcggccag ggcatcgcgg cgatcttcga gcgcgtctga atccggcgcg ccatggactt
     1021 cgacatcccc gcacccctgc gcgagctgca gcagcgcacc cgccgcttca tcgccgacga
     1081 agtgatcccg ctcgaaaacg acgcgcgctg caccccgcac ggccccggcg agcccttgcg
     1141 cgccgagctg atcgcccgcg ccgcggcggc cggcctgctt gcgccccacg tcggccgcga
     1201 cttcggcggg ctggggctgg accaccgcgg caaggccatc gtcttcgagg aggccggcta
     1261 ctcgccgctc ggcccggtgg cgctgaacat catggcaccc gacgagggca acatgcatct
     1321 gctcgaggca gtggccggcc cggcccagca ggagcgctgg ctgcggccgc tggcggccgg
     1381 ccgcacgcgc tcgtgcttct gcatgaccga gcccgacccc ggtgccggct ccgaccccgg
     1441 catgctgcag acccgcgcgg tgcgtgacgg cgacgactac ctcatcagcg ggcgcaagtg
     1501 gttcatcacc ggcgccgacg gcgccgcgtt cgcgatcatc atggcccggc tcgaggacgg
     1561 ccgcgcaacg atgttcctgg ccgacatgga ccggccgggc atcaccgtcg agcgttcgat
     1621 ggacacgctc gacagctgct tcccgggcgg gcacggcgtg gtgcgcttcg acgagctgcg
     1681 cgtgccggcg gccgacgtgc tcggcgcgcc gggcgagggc ttcaaatacg cgcaggtgcg
     1741 cttgtcgccg gcgcggctga cgcactgcat gcgctggctc ggcgccgcgc ggcgcgcgca
     1801 cgacacggcc gtggcctacg cccgccgacg gcacgccttc ggcaagccgc tggccgagca
     1861 cgaaggcgtc ggcttcggcc tggccgacaa cgagatggac ctgcacctca cgcggctgtc
     1921 ggtctggcac accgcctggg tgctcgatca gggccacctg ggcacgaccg agtcgagcat
     1981 gtgcaaggtg atcgcctcgg aggcgatctg gcgcgtcgtc gaccgcagcg tgcagttcct
     2041 gggtggccag ggcgtcaccg gcgagacgat cgtcgcgcgc atctttgctg acgtgcgcgc
     2101 cttccgcatc tacgacggtc cgtcggaagt gcaccgctgg agcctggcca ggcgcatctt
     2161 caaaggcacc gtgacgtgaa cgacgcgcag tgcgccgcgc tcgagcgctt catcgcgcgg
     2221 gcggccgcgg ccgagcgg
//
LOCUS       DATMJB010000293         2390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_595408, whole genome shotgun sequence.
ACCESSION   DATMJB010000293 DATMJB010000000
VERSION     DATMJB010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595408"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..369)
                     /locus_tag="VNU48_06495"
     tRNA            complement(294..369)
                     /locus_tag="VNU48_06495"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(334..336),aa:His,seq:gtg)
     gene            complement(424..2352)
                     /locus_tag="VNU48_06500"
     CDS             complement(424..2352)
                     /locus_tag="VNU48_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HWI10961.1"
                     /translation="MFDFFRKHTWLLQLILGFVVLAFVGGGVYQGYGSFMRDEGSTVA
                     KVDGRKITRTEWEVAQREQIERVRRQMPNVDAKLFDTPEMKRQSLDAVVRDRVMLTAA
                     DKLHLATTDDRLQRLFATDPQLAFLRNPDGSVNKDALAAQGMSSEMFAQRLRQELSLR
                     QVVSGISATVTAPLSATNAALDAMFQQREVQVQRFATKDSVANVAPTDADIDKYYKDP
                     ANASQFMAPEVADVEYVVLDLDALKKGITVSDKELRDYYAANEARYTTPEERRASHIL
                     VKVDAGATAADRAKAKAKAEALLAEVRKSPASFAEVARKNSDDPGSAEKGGDLDFFGR
                     GGLAAKPLEDAAFALKPDEISALVESDFGYHIVRLTAVRGGDKRSFDAVRAELEGEVK
                     TQLAQKRFSEAAVDFTNMVYEQPDSLKPVIDKLKLEPRRAQNIKRAPAPGASGALANP
                     KFLDALFGTDAIRNKRNTEAIEIGASTLVSGRVVKYEAAHQLPLADVKAQVRERLVTL
                     QAAALARKLGQARLAELRAAPGTAMPELAMTLSRAQSRDLPGALVDAVLKAPVATLPA
                     FVGVDLGDQGYAVAKIAKVLGRDPATADATRAQAEYARAWGDAESLAYYAALKSRYKV
                     EIRPDVVAAADAASTASK"
BASE COUNT          347 a          827 c          819 g          397 t
ORIGIN      
        1 cgggctgggc ggcgtgcgca agcgcgtcaa gcagctcggc ggccaggtcg aatggcgcga
       61 ggtcgcgccg cacggcatca gctgccgcgt gatcatccgc aacctgtcgg cgcgcggtta
      121 gcggccgcga tgccgcgcgc gatcgctcca tggaaaaggc ctcggatcgc tccaaggccc
      181 tggtgacgca cgctgaattc tggggtggct gatggggctc gaagcctaac tccctaaccc
      241 acagctaacc cacggaccaa gaagagacgc cgcatcttca taggtaaaat gggtggggtg
      301 gctgatgggg ctcgaaccca cgacaacagg aatcacaatc ctggactcta ccaactgagc
      361 tacagccacc gtagaggccc gcattatagc ggatgcctct gcgccgaagg ctgcgtggcg
      421 aggctacttc gaggccgtcg acgccgcatc ggcggccgcg accacgtccg gcctgatctc
      481 gaccttgtag cgcgacttca gcgccgcgta gtaggccagc gactccgcgt cgccccaggc
      541 gcgcgcgtac tcggcctgcg cacgcgtcgc atcggcagtg gccgggtcgc ggcccagcac
      601 cttggcgatc ttcgcgaccg cgtagccctg gtcgccgagg tcgacgccga cgaaggccgg
      661 cagcgtggcc accggcgcct tcagcacggc gtcgaccagc gcgcccggca gatcgcgtga
      721 ctgggcgcgc gacagcgtca tggccagctc cggcatcgcc gtgcccggcg ccgcacgcag
      781 ctcggccagc cgtgcctgcc cgagcttgcg cgcgagcgcc gccgcctgca gcgtgaccag
      841 ccgttcgcgg acctgcgcct tcacgtcggc cagcggcagc tggtgcgcgg cctcgtactt
      901 gacgacgcgg cccgagacca gcgtgctcgc gccgatctcg atcgcttcgg tgttgcgctt
      961 gttgcggatc gcgtcggtgc cgaacagtgc gtcgaggaac ttcgggttcg ccagcgcgcc
     1021 gctcgcgccc ggcgccggcg cgcgcttgat gttctgcgcg cggcgcggct cgagcttgag
     1081 cttgtcgatc accggcttca ggctgtcggg ctgctcgtag accatgttgg tgaagtcgac
     1141 cgccgcctcg gaaaagcgct tctgcgcgag ctgcgtcttg acctcgcctt cgagttcggc
     1201 cctgaccgca tcgaagctgc gcttgtcgcc gccgcgcacc gcggtgagcc tgacgatgtg
     1261 gtagccgaag tcgctctcca ccagggcgct gatctcgtcg ggcttgagcg cgaaggccgc
     1321 atcctcgagg ggcttggcgg ccaggccgcc acggccgaag aagtcgaggt cgccgccctt
     1381 ctcggccgag cccgggtcgt cggagttctt gcgcgcgacc tcggcgaacg atgccgggct
     1441 cttcctgacc tcggccagca gggcctcggc cttggccttc gccttggcgc ggtcggcagc
     1501 cgttgcgccg gcatcgacct tgaccaggat gtggctggcg cggcgctcct cgggcgtggt
     1561 gtagcgcgcc tcgttggcgg cgtagtagtc gcgcagttcc ttgtcggaga ccgtgatgcc
     1621 cttcttcagc gcgtcgaggt cgagcacgac gtactcgacg tccgcgacct cgggcgccat
     1681 gaactgcgag gcgttcgccg ggtccttgta gtacttgtcg atgtcggcat cggtcggcgc
     1741 gacgttcgcg accgagtcct tggtcgcgaa gcgctgcacc tgcacctcgc gctgctggaa
     1801 catcgcgtcg agcgcggcgt tggtcgcgct caacggcgcg gtcaccgtgg cgctgatgcc
     1861 cgacacgacc tgacgcaagg acagctcctg gcgcagccgc tgcgcgaaca tctccgacga
     1921 catgccctgg gccgccagcg cgtccttgtt gacgctgccg tcgggattgc gcaggaacgc
     1981 gagctgcggg tcggtcgcga acaggcgctg caggcggtcg tcggtcgtgg ccaggtgcag
     2041 cttgtcggcg gcggtgagca tcacgcggtc gcgcaccacg gcgtcgagcg actggcgctt
     2101 catctccggc gtgtcgaaca gcttggcgtc gacattgggc atctggcgcc gcacgcgctc
     2161 gatctgctcg cgctgcgcga cctcccattc ggtgcgcgtg atcttgcgcc cgtcgacctt
     2221 cgcgaccgtc gagccctcgt cacgcatgaa gctgccgtag ccctggtaga cgccgccacc
     2281 gacgaacgcg agcacgacga agcccagaat caattgcagc aaccaggtgt gcttgcggaa
     2341 gaaatcaaac atcgaaaagc ctctgtcgac gggccgcccg cgccgcaggg
//
LOCUS       DATMJB010000294         2824 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_546370, whole genome shotgun sequence.
ACCESSION   DATMJB010000294 DATMJB010000000
VERSION     DATMJB010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546370"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VNU48_06505"
     CDS             complement(<1..437)
                     /locus_tag="VNU48_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HWI10962.1"
                     /translation="MLKTTDLARLSRALDERFADAPGLFDNDPVAIDLAQVRDAEEPI
                     DFVVLIHLLRGYKMLPVAVKSGNAQQMEDAHAAGLSEAHGGGPGALPVRQHAPVDPSA
                     APAPVPPPSVDAELPEVTEIAVPARSALVVDKPLRSGQRVYAKG"
     gene            complement(636..1103)
                     /locus_tag="VNU48_06510"
     CDS             complement(636..1103)
                     /locus_tag="VNU48_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HWI10963.1"
                     /translation="MIRALAFFLILVLVGLFALLNWDAFSALTTLNLGLTTLQAPLGL
                     IMLGLVGFLCVLFTVWVISLQAGSLMDARRQTRELQAQRDLADRAEASRFTELRSELM
                     VRLDRLQSELRLGVEQTANEVAAHIGQLEDRLERARLLRPTSALESADHARVP"
     gene            complement(1235..>2824)
                     /gene="acpA"
                     /locus_tag="VNU48_06515"
     CDS             complement(1235..>2824)
                     /gene="acpA"
                     /locus_tag="VNU48_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HWI10964.1"
                     /translation="AAVAEAGAQGDRARALRNHLALDAIETLVVIYAENRAFDTLYGL
                     FPGANGVPGKNPSSTGGYAPQRDFDGAVLPVLPPAWGGLTAGGQPVTLTQAQSTGMPN
                     APFQIDDPAGLNGSGVVVGQNIATRDLVHRFYSNIMQINGGRNDRFAAFSDAGALSMG
                     YYDGSRMKLWALARQYTLADNFFMGAFGGSFLNHQYLVCACAPVYPNADAADSPAKDS
                     ISQIETDAQGRFKRLVPADTMPATALAGAPRYRRDANLTPKDASGMFYAVNTMQPAFQ
                     PSFNAPAAGGDARYADRSKPTTLPPQTLPTIADALDAKGVAWAWYAGGWNAVETGTPE
                     ARKQMYSGTVNFQPHHQPFNYYAAFDPATHAAARQAHLKDFDTGFLADAAAGRLPPVT
                     FYKPQGPLNQHAGYADVAAGDAHIAEVIATLQRSPQWPHMLIVVTYDENGGFYDHAAV
                     PAGDWLGPGTRIPALIVSPFAKKGFVDKTPYDTGSILRFITHRWSLDSLPGIAMRDAA
                     LARAGSAPMGDLSGALDFVGH"
BASE COUNT          397 a          960 c         1052 g          415 t
ORIGIN      
        1 cccttcgcat agacgcgctg gcccgagcgc aagggcttgt cgaccaccag cgcgctgcgc
       61 gccggcaccg cgatttcggt gacctcgggc agctccgcgt caacgctcgg cggcggcacc
      121 ggcgcaggtg ccgccgacgg gtcgaccggc gcgtgctgac gcaccggcag cgcgccgggg
      181 ccgccgccat gcgcctcgct caggccggcg gcgtgggcgt cctccatctg ctgggcgttg
      241 ccggacttga ccgcgaccgg cagcatcttg tagccgcgca gcaggtggat cagcacgacg
      301 aagtcgatcg gctcttcggc atcgcgcacc tgggcgaggt cgatcgccac cgggtcgttg
      361 tcgaacagcc ccggggcgtc ggcgaagcgc tcgtcgagcg cccgggagag ccgagcgagg
      421 tcggtcgtct tgagcacgac ggcgaccagt gtcagggcgg cgcttttcag ttcaaaggcg
      481 gaagacgcgg cagaagcaac aaccatgagc gcagacggcg gcaaaggctg cgagtttagc
      541 ggctgcgcag gccctgtctg ttcgcaagcg tgtgcgggcg ttgcccgccg cgcacgcagg
      601 cgccgctcac gcgaccctgc gtcggccggg atgggtcacg gcacgcgtgc gtgatcggcc
      661 gattcgagcg cgctcgtcgg ccgcagcagc cgggcgcgtt cgagccggtc ctcgagctgg
      721 ccgatgtgcg cggcgacctc gttcgcggtc tgctcgacgc ccaggcgcaa ttcgctctgc
      781 agccggtcca gccgcaccat cagctcgctg cgcaactcgg tgaagcgcga ggcctcggcc
      841 cggtcggcga ggtcgcgctg ggcctgcagc tcgcgcgtct ggcggcgcgc gtccatcagc
      901 gagccggcct gcagcgagat cacccacacg gtgaacagca cgcacaggaa cccgaccagc
      961 cccagcatga tcaggccgag cggcgcctgc agcgtcgtca ggcccaggtt cagcgtcgtc
     1021 aacgcggaaa acgcgtccca gttcagcagc gcgaacaggc cgaccaggac caggatcaga
     1081 aagaatgcga gtgcgcgaat catcgtgacc tccgagtctg tcagagcggc taggttagcg
     1141 ggccgcgcgc ggctggcaag cggcgcggca cccgcggcgc gtcggaggcg gcgcactcgt
     1201 gttgccggac gacatgccgt ccggcccctc gcggtcagtg cccgacgaag tcgagtgccc
     1261 cgctcaggtc gcccatcggc gcgctgccgg cgcgcgccag agcggcgtcg cgcatcgcga
     1321 tgcccggcag cgagtcgagc gaccagcggt gcgtgatgaa gcgcaggatc gagccggtgt
     1381 cgtagggtgt cttgtcgacg aagcccttct tcgcgaacgg cgagacgatc agcgccggaa
     1441 tgcgcgtgcc cgggccgagc cagtcgcccg ccggcaccgc ggcatggtcg tagaagccgc
     1501 cgttctcgtc gtaggtcacg acgatcaaca tgtgcggcca ctgcgggctg cgctgcagcg
     1561 tggcgatgac ctcggcgatg tgcgcgtcgc cggcggccac gtcggcatag ccggcgtgct
     1621 ggttcagcgg gccctgcggc ttgtagaacg tgaccggcgg cagcctgccg gcggcggcgt
     1681 cggcgaggaa gccggtgtcg aagtccttca ggtgcgcctg gcgcgcggcc gcgtgcgtgg
     1741 ccgggtcgaa cgcggcgtag tagttgaagg gctggtgatg cggctggaag ttgaccgtgc
     1801 cgctgtacat ctgcttgcgt gcctcgggcg tgccggtctc gaccgcgttc cagccgcccg
     1861 cgtaccaggc ccaggccacg cccttcgcgt cgagcgcgtc ggcgatcgtc ggcagggtct
     1921 gcggcggcag cgtggtcggc ttgctccggt cggcatagcg cgcgtcgcca ccggcggcag
     1981 gcgcgttgaa gctcggctgg aacgccggct gcatcgtgtt gacggcgtag aacatgccgc
     2041 tcgcgtcctt cggcgtcagg ttggcgtcgc gccggtagcg cggcgcgccg gcgagcgcgg
     2101 tcgcgggcat ggtgtcggcg ggcacgaggc gtttgaaccg gccctgcgcg tcggtctcga
     2161 tctgcgagat cgaatccttc gcgggcgaat cggcagcgtc ggcgttcggg tagaccggcg
     2221 cacaggcgca gaccaggtac tggtggttca ggaacgagcc gccgaacgcg cccatgaaga
     2281 agttgtcggc cagcgtgtac tgccgcgcca gcgcccacag cttcatgcgg ctgccgtcgt
     2341 agtagcccat gctcagggcc ccggcgtcgg agaacgcggc gaagcggtcg ttgcgcccgc
     2401 cattgatctg catgatgttg ctgtagaagc gatgcaccag atcgcgcgtc gcgatgttct
     2461 ggccgaccac cacgccgctg ccgttcaggc ccgcgggatc gtcgatctgg aacggcgcgt
     2521 tcggcatgcc ggtggactgt gcctgcgtca aggtgaccgg ctggccgccc gcggtcagcc
     2581 cgccccaggc cggcggcagc accggcagca ccgcgccgtc gaagtcgcgc tgtggcgcgt
     2641 agccgccggt cgagctcggg ttcttgcccg gcacgccgtt ggcaccgggg aacaggccgt
     2701 agagcgtgtc gaacgcgcgg ttctcggcat agatcacgac gagggtctcg atcgcgtcga
     2761 gcgcgaggtg gttgcgcagc gcgcgcgcgc ggtcgccctg cgcgccggcc tcggcgaccg
     2821 cggc
//
LOCUS       DATMJB010000295         2853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_535866, whole genome shotgun sequence.
ACCESSION   DATMJB010000295 DATMJB010000000
VERSION     DATMJB010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535866"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="VNU48_06520"
     CDS             <1..1558
                     /locus_tag="VNU48_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HWI10965.1"
                     /translation="SIAVPGDRGAHISLSLVSHTNAGKTTLARTLLGRDVGAVRDAPH
                     VTEFADVFTMLETPAGERLLLWDTPGFGDSARLVKRLRGSANPVGWFVAEVWDRWRDR
                     AFWASQQAMRNVRVEADVMLYLVNAAESPEAAGYVAPEMELLGWIAKPVIVLLNQLGA
                     PRERALEAAEVERWRRHLAPHAQVKAVLPLDAFARCWVQEGTLLRAVEAALDGPRRAL
                     MGRLRAAWQAQRLQTFDAAMRSIAASLARLAGAAEEVPDPQRLGARLRRAGSAIGSSL
                     GLREAELAPTALAQQALAERLAAELRTNTAELIALHGLEGRAQGEILARLATHFETRL
                     RMDEGKAALWGGMVSGALVGLKADLLSGGLTLGGGLLAGGLIGALGAAGLARCVNLVR
                     GTERSYVAWNAEALDQLLEAALLRYLAVAHFGRGRGEWSQGESPAHWREVVQRALAPQ
                     REALAQVWQERDVGRGSDAGAGAAEAERLIAAALLPVVGAAAGDALRSLYPDAASLGE
                     SAQPEPQASQ"
     gene            1570..2181
                     /locus_tag="VNU48_06525"
     CDS             1570..2181
                     /locus_tag="VNU48_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HWI10966.1"
                     /translation="MNAPHRAVEFYFDFSSPYSYIASEWIEALAARHGRVVNWRAILL
                     DATFQVTAIKSPVSYPLKREYTLLDFERSARFAGVPLHLPEDFPIDTINAARIFWWLH
                     AADPGRARDWARHALRALFARGVDLGDPPSLRALAVEFGLDGSQAQDAWNDPQWKAAL
                     KAANDAAIALGVFGAPFFIIDGEPFWGNDRRAQIERWLEKGAF"
     gene            2273..>2853
                     /locus_tag="VNU48_06530"
     CDS             2273..>2853
                     /locus_tag="VNU48_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWI10967.1"
                     /translation="MPALASKLNARTEDFKANAAAMRALVTDLNAKLAQVALGGGEAP
                     RAKHVARGKLLPRERVEMLLDPDTPFLELAPLAALEMYENAAPGAGLIAGIGRVSGVD
                     CMIVCNDATVKGGTYYPMTVKKHLRAQEIAQQNRLPCIYLVDSGGANLPNQDEVFPDR
                     DHFGRIFYNQANLSAQGIAQIAVVMGSCTAGGA"
BASE COUNT          392 a         1033 c         1031 g          397 t
ORIGIN      
        1 atcgattgcc gtacccggcg atcgaggggc gcacatctcg ctgagcctgg tgtcgcacac
       61 caacgccggc aagacgacgc tcgcgcgcac gctgctcggc cgcgatgtcg gcgcggtgcg
      121 cgacgcgccg cacgtcaccg agttcgccga tgtcttcacg atgctcgaga cgcccgccgg
      181 tgaacgcctg ctgctgtggg acacacccgg cttcggcgac agcgcgcggc tcgtgaagcg
      241 gctgcgcggc agcgccaacc cggtcggctg gttcgtcgcc gaggtgtggg accgctggcg
      301 cgaccgcgcg ttctgggcca gccagcaggc gatgcgcaac gtgcgcgtcg aggccgacgt
      361 gatgctctac ctcgtcaacg ccgccgagtc gccggaagcg gccggctacg tggcgcccga
      421 gatggagctg ctgggctgga tcgccaaacc cgtgatcgtg ctgctgaacc agctcggcgc
      481 gccgcgcgag cgtgcgctcg aggccgccga ggtcgagcgc tggcgccgcc acctcgcgcc
      541 gcacgcgcag gtgaaggcgg tgctgccgct ggatgcgttc gcgcgctgct gggtgcagga
      601 gggcacgttg ttgcgagcgg tcgaggccgc gctcgatggt ccacgccgcg cgctgatggg
      661 ccggctgcgc gcggcctggc aggcgcagcg cctgcagacc ttcgacgccg cgatgcgctc
      721 gatcgccgcg agcctggcgc gcctggccgg cgcggccgag gaggtgccgg acccgcagcg
      781 cctgggcgcg cgactgcgcc gcgccggcag cgcgatcggc agctcgctgg gcttgcgcga
      841 ggccgaactg gcgccgaccg cgctggccca gcaggcgctg gccgaacggc tggccgccga
      901 gctgcgaacc aacaccgccg agctgatcgc gctgcacggc ctcgaaggcc gcgcgcaggg
      961 cgagatcctg gcgcgcctgg cgacgcactt cgaaacccgc ttgcgcatgg acgaaggcaa
     1021 ggcggcgctg tggggcggca tggtcagcgg cgcgctggtc gggctgaagg ccgacctgct
     1081 cagcggcgga ctcacgctcg gcggcggcct gctcgccggc ggcttgatcg gcgcgctcgg
     1141 cgcggccggc ctggcgcgtt gcgtgaacct ggtgcgcggc accgagcgtt cgtacgtcgc
     1201 ctggaatgcc gaggcgctgg accagttgct cgaggccgcg ctgctgcgct atctggcggt
     1261 cgcgcatttc ggccgcgggc gcggcgagtg gtcgcagggc gaatcgcccg cgcactggcg
     1321 cgaggtggtg cagcgcgcac tggcgccgca acgcgaggcg ctggcccagg tctggcagga
     1381 acgcgacgtt gggcgcggca gcgatgccgg tgccggcgcg gccgaggccg agcgtctgat
     1441 cgccgcggcc ttgctgcccg tcgtcggcgc ggccgcgggc gacgcgctgc gcagcctcta
     1501 ccccgacgcc gcgtcgcttg gggaaagtgc ccaacccgag ccgcaggctt cgcagtagcc
     1561 ttggggcgaa tgaacgcccc gcaccgcgcc gtcgagttct acttcgactt ctcgtcgccg
     1621 tactcgtaca ttgcctccga gtggatcgag gcgctcgcgg cgcgtcacgg ccgggtcgtg
     1681 aactggcgcg cgatcctgct cgacgcgacc ttccaggtca ccgcgatcaa gagcccggtg
     1741 tcgtacccgc tgaagcgcga gtacacgctg ctcgacttcg aacgctcggc gcgcttcgcc
     1801 ggcgtgccgc tgcacctgcc cgaggacttc ccgatcgaca cgatcaatgc cgcccgcatc
     1861 ttctggtggc tgcacgccgc cgacccgggc cgtgcgcgcg actgggcacg ccacgcgctg
     1921 cgcgcgctgt tcgcgcgcgg cgtcgacctc ggcgacccgc cctcgttgcg cgccctggcc
     1981 gtcgagttcg gactcgacgg ttcgcaggcc caggacgcct ggaacgaccc gcagtggaag
     2041 gccgcgttga aggccgcgaa cgatgcggcg atcgcgctcg gcgtgttcgg cgcgccgttc
     2101 ttcatcatcg acggcgagcc cttctggggc aacgaccggc gagcgcagat cgaacgctgg
     2161 ctcgagaagg gcgcgttcta gccccgcggc gctgcgttcg acgcgcggcg cctacaattg
     2221 acgtatacgt caactgcgca ccttcggcgt ctctcgcgcc acgaaagccc gcatgcctgc
     2281 actcgcctcc aagctcaacg cccgcaccga ggatttcaag gccaacgccg cggcgatgcg
     2341 tgcgctcgtg accgacctga acgccaagct cgcgcaggtg gcgctgggcg gcggcgaggc
     2401 gccgcgcgcc aagcacgtgg cgcgcggcaa gctgctgccg cgcgagcgcg tcgagatgct
     2461 gctcgatccg gacacgccgt tcctcgaact cgcgccgctc gccgcgctcg agatgtacga
     2521 gaacgccgca ccgggcgccg ggctgatcgc cggcatcggc cgcgtcagcg gcgtcgactg
     2581 catgatcgtg tgcaacgacg cgaccgtgaa gggcggcacc tactacccga tgacggtgaa
     2641 gaagcacctg cgcgcccagg agatcgcaca gcagaaccgc ctgccctgca tctacctcgt
     2701 cgacagcggc ggcgccaacc tgcccaacca ggacgaggtg ttcccggacc gcgaccactt
     2761 cggccgcatc ttctacaacc aggccaacct gtcggcgcag ggcatcgctc agatcgcggt
     2821 ggtcatgggc tcctgcaccg ccggcggcgc cta
//
LOCUS       DATMJB010000296         2310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_535799, whole genome shotgun sequence.
ACCESSION   DATMJB010000296 DATMJB010000000
VERSION     DATMJB010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535799"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1923)
                     /locus_tag="VNU48_06535"
     CDS             complement(67..1923)
                     /locus_tag="VNU48_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HWI10968.1"
                     /translation="MSEAAATVRYRAVPLGGSLAARFAPRADGGTIVTSTEALLPYAA
                     RLTDRLLHWAEHAPERTLVAKRVDGGDWRRVSYAQALVAARSIAQALIDHGLSAERPL
                     AILSDNDIEHLLLGLGAMLAGVPFAPISAAYSLLSQDHGKLRHIVGTLTPGMVFAADG
                     AAFAKAIDAVVPADTPVVLTRGAIAGRATLAFADLLATRATPQVDAAHAAVGPDTIAK
                     FLFTSGSTKLPKGVINTQRMLCSNQQMIRQCFPQLLDEPPVLVDWLPWNHTFGGNHNV
                     GIVLYNGGTLYIDEGKPTPALIGETLRNLREIAPTMYFNVPKGFEEIANALEVDAQLR
                     ATLFSRVKMFFFAGAGLAQPVWDKLDRVAEAHAGERIRMLTGLGMTETAPFAVCANAH
                     EVKSGHIGLPAPGIELKIEPQGDKFEMRYRGPNVTPGYWRAPAQTAESFDAEGFYCSG
                     DAVKPMDAAQPQRGFMFDGRIAEDFKLATGTFVSVGPLRAKIIAAGDPCVQDVVVAGI
                     NRNELGILIFPRLDACRALAGAAPNEPAERVLAEPPIRAFFQRLVDDLHAGGTGSATR
                     VARALVLTEPPSIDRGEITDKGSINQRAVLTHRAALVEQLYADAPGVIRPRG"
     gene            complement(1920..>2310)
                     /locus_tag="VNU48_06540"
     CDS             complement(1920..>2310)
                     /locus_tag="VNU48_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWI10969.1"
                     /translation="PEGQRGLFVGGGGSARIPRLIGVARMTDMMLTGRVIDAEEGGRI
                     GLSQYVVDDGEGLAKALALAEKVAANAPLSNFAVVHALPRIAEMGQDEGLFTESLMAA
                     IAQGDDAAKGRMRAFLDGKAGKVGKKS"
BASE COUNT          316 a          814 c          835 g          345 t
ORIGIN      
        1 gaggcgccgc cggtcacgag cgccgcttgc ccctggatgt tcatcgtcgt gtttcccgaa
       61 gaggaatcag ccgcgcggcc ggatcacgcc cggcgcgtcg gcatacagct gctcgaccag
      121 cgcggcgcgg tgcgtcagca ccgcgcgctg gttgatcgag cccttgtcgg tgatctcgcc
      181 gcggtcgatg ctgggcggct cggtcagcac cagcgcgcgg gcgacgcgcg tcgcgctgcc
      241 ggtgccgccg gcgtgcagat cgtcgacgag ccgctggaag aacgcgcgga tcggcggctc
      301 ggccagcacc cgctcggccg gctcattcgg cgcggcgccg gcgagcgcgc ggcacgcgtc
      361 caggcgcggg aagatcagga tgccgagctc gttgcggttg atcccggcga cgacgacgtc
      421 ctgcacgcag gggtcgcccg cggcgatgat cttcgcgcgc agcgggccga ccgagacgaa
      481 ggtgccggtc gcgagcttga agtcctcggc gatgcggccg tcgaacatga agccgcgctg
      541 gggctgcgcc gcgtccatcg gcttgaccgc gtcaccgctg cagtagaagc cttcggcgtc
      601 gaacgactct gccgtctgcg cgggcgcacg ccagtagccg ggcgtgacgt tcgggccgcg
      661 gtagcgcatc tcgaacttgt cgccctgcgg ctcgatcttc agctcgatgc cgggcgccgg
      721 cagcccgatg tggccggact tgacctcgtg cgcgttcgcg cacaccgcga acggcgcggt
      781 ctcggtcatg cccagtccgg tgagcatgcg gatgcgctcg ccggcatgcg cttcggcgac
      841 gcggtcgagc ttgtcccaga ccggctgcgc caggccggcg ccggcgaaga agaacatctt
      901 cacgcggctg aacagcgtcg cgcgcagctg cgcatcgacc tcgagcgcgt tggcgatctc
      961 ctcgaagccc ttgggcacgt tgaagtacat cgtcggcgcg atctcgcgca gattgcgcag
     1021 ggtctcgccg atcagcgccg gcgtcggctt gccctcgtcg atgtagagcg tgccgccgtt
     1081 gtagagcacg atgccgacgt tgtggttgcc gccgaaggtg tggttccagg gcagccagtc
     1141 gaccagcacc ggcggctcgt ccagcagctg cgggaagcac tggcggatca tctgctggtt
     1201 gctgcacagc atgcgctggg tgttgatcac gcccttgggc aatttggtcg agcccgaggt
     1261 gaacaggaac ttcgcgatcg tgtcggggcc gaccgccgcg tgcgcggcgt cgacctgagg
     1321 cgtggcacgc gtcgcgagca ggtcggcgaa cgccagcgtc gcgcgcccgg cgatcgcgcc
     1381 gcgcgtcagc acgacgggtg tgtcggccgg cacgaccgcg tcgatcgcct tcgcgaacgc
     1441 cgcgccgtcg gccgcgaaca ccatgcccgg cgtcagcgtg ccgacgatgt gacgcagctt
     1501 gccgtggtcc tgcgacagca gcgagtacgc ggccgagatc ggcgcgaacg gcacgccagc
     1561 gagcatcgcg ccaaggccga gcagcaggtg ctcgatgtcg ttgtcggaca ggatcgccag
     1621 cgggcgctcg gcgctcaggc cgtggtcgat cagcgcctgc gcgatcgaac gcgctgccac
     1681 cagcgcctgc gcataactga cgcgccgcca gtcgccgccg tcgacgcgct tggcgaccag
     1741 cgtgcgctcg ggcgcgtgct cggcccagtg cagcaggcgg tcggtcaggc gcgccgcgta
     1801 gggcagcagc gcttcggtcg acgtgacgat cgtgccgccg tcggcgcgcg gcgcgaagcg
     1861 cgccgcgagc gagccgccca gcggcaccgc gcggtagcgc accgtcgcgg cggcctcgct
     1921 catgatttct tgcccacctt gccggccttg ccgtcgagga acgcgcgcat gcggcccttc
     1981 gcggcgtcgt cgccctgcgc gatcgcggcc atcagcgatt cggtgaacag accttcgtcc
     2041 tgccccatct cggcgatgcg cggcagcgcg tgcaccacgg cgaagttgga cagcggcgca
     2101 ttcgcagcga ccttctcggc cagcgcgagt gccttggcca ggccctcgcc gtcgtcgacc
     2161 acgtactgcg acaggccgat gcggccgcct tcttcggcat cgatgacgcg gccagtcagc
     2221 atcatgtcgg tcatgcgcgc gacgccgatc aggcgcggga tgcgcgccga cccgccgccg
     2281 ccgacgaaca ggccgcgctg gccttcgggc
//
LOCUS       DATMJB010000297         3873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_324866, whole genome shotgun sequence.
ACCESSION   DATMJB010000297 DATMJB010000000
VERSION     DATMJB010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324866"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2140)
                     /locus_tag="VNU48_06545"
     CDS             complement(<1..2140)
                     /locus_tag="VNU48_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWI10970.1"
                     /translation="MHDATDPARTPGLNNTWPGRASPTWTGHPSQLGDSGLDSGVGER
                     GAEQALPAARWMGWRLRLLFTVALLGCIGLYLLIRAMALAPHIDARWRIDAQGRIELA
                     ASDDPALRMQVGQVLRGATGGDATVVLSDAPVLQRSPRWVIDDDERARQRVLLEQLAA
                     VSAHREVTLFFADGSVAEVQSGPRGLLGLPILFWVLSAFALGLYLVAMVVPLANPSGR
                     NLIYAVMALCQCGNLLAIAVECVFDFGLPAPLARWDMPVRAGFDLFTAAAMVNAVCLH
                     PRRLPGSGWIALAGWALVLTLVGCIAADALVNAWWLTQTAVIGLGLVAIGLLSWSYRI
                     ERHPFAIVLRRFAVVTVVTWALLTAADRLPGLPYNIGDVGSLVWYVFLASLLLLVPFL
                     ARSQDFMREFSLLAAISTVATSLDLLFVSVFSLGQFASLTLSLFVSLAVYSGVRQWIL
                     NRLLGSSMLTTERMFEQLYRIAREVEAQPERTPALLSQLLGDLFEPLELNVVERHTAK
                     TRVAGDGSAMLVPVPLLGGETTPRGSVMVRFAQRGRRLFTSEDARLTDRIVEQLRRAV
                     HFDKAVEQGRSEERLRLAQDLHDDIGARLLTLMYKAQSPEMEDYVRHTLQDLKTLTRG
                     LAASNHRLSHAAAEWKTDLAHRLTAAHVDLTWTFVFDEDILLTVVHWSALTRILRELV
                     SNAIAHSQARTLDIDFRLESDRIELNITDDG"
     gene            complement(2163..2900)
                     /locus_tag="VNU48_06550"
     CDS             complement(2163..2900)
                     /locus_tag="VNU48_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI10971.1"
                     /translation="MPVKVLIVEDNPVARSFLSRVVRESFSDALIITEARDLETARRQ
                     IGPLRDAAQAPDPFKLILVDLELPDGNGMELLTELGTYPATKIVTTLYSDDDHLFPAL
                     QCGTDGYLLKEDRFEVLVEELQKIVRGQPPLSPAIARRLLTHFRHGPGGGSAPNSGPV
                     PLVSGFQSSRPVPMGRGAPLDHERLTPRESEVLTYLSKGFTIKEIANLMGIKWFTVND
                     HIKSIYKKLNVSSRAEAAVLASKQGLV"
     gene            complement(2977..>3873)
                     /locus_tag="VNU48_06555"
     CDS             complement(2977..>3873)
                     /locus_tag="VNU48_06555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HWI10972.1"
                     /translation="DEVRVAIRAASLNFPDILIVQNKYQLKPPLPFVPGSEYAGVVEA
                     VGAGVTQLKVGDAVAACGATGGFATHAVVKAALVMPLPPGFAFDDASAFVLTYATTHH
                     ALLDRAALKPGETVLILGAAGGVGTAAIQIAKAVGARVIAAASSDAKCELCKSLGADA
                     TINYSKANLRDEIKALTDGKGPDVVYDPVGGDLAEPVFRSIAWRGRYLVVGFAQGGIP
                     ALPLNLALLKGASIVGVFWGEFMRREPKANAKALAELAQWYARGLVKPVIDRRLPMDQ
                     LLQAFEVMAGRQVRGKLVMTNP"
BASE COUNT          615 a         1347 c         1356 g          555 t
ORIGIN      
        1 cgccgtcgtc ggtgatgttc agctcgatgc ggtcgctctc gaggcggaag tcgatgtcga
       61 gcgtgcgcgc ctgcgaatgc gcgatcgcgt tgctgaccag ctcgcgcagg atgcgcgtca
      121 gcgccgacca gtgcaccacc gtcagcagga tgtcttcgtc gaacacgaag gtccaggtca
      181 ggtcgacgtg cgcggccgtc aggcggtgcg ccaggtccgt cttccattcg gcggcggcgt
      241 gcgacagccg gtggttcgat gcggccagac cacgcgtgag cgtcttcagg tcctgcagcg
      301 tgtggcgcac gtagtcttcc atctcgggcg actgggcctt gtacatcagg gtcagcaggc
      361 gcgcgccgat gtcgtcgtgc aggtcctgcg ccaggcgcag ccgctcttcg ctgcggccct
      421 gttcgacggc cttgtcgaag tgcacggcgc gtcgcagctg ctcgacgatg cggtcggtga
      481 ggcgcgcgtc ttccgacgtg aacaggcgcc gtccgcgctg cgcgaagcgc accatcaccg
      541 agccgcgcgg ggtggtctcg ccgccgagca gcggcaccgg cacgagcatg gccgagccgt
      601 cgccggcgac gcgcgtcttg gcggtgtgcc gctcgacgac gttgagttcg agcggctcga
      661 acagatcgcc cagcaattgc gacagcagcg ccggcgtgcg ctccggctgc gcctcgacct
      721 cgcgcgcgat gcggtacagc tgctcgaaca tgcgctcggt cgtcagcatg ctgctgccca
      781 gcagccggtt caggatccac tggcgcacgc ccgaatacac cgccagcgag acgaacagcg
      841 acagggtcag cgaggcgaac tggccgagcg agaacaccga cacgaacagc aggtcgagcg
      901 aggtcgcgac ggtgctgatc gccgccagca gcgagaactc gcgcatgaag tcctgcgagc
      961 gcgcgaggaa gggcaccagc agcagcagcg aggccaggaa cacgtaccag accagcgagc
     1021 cgacgtcgcc gatgttgtac ggcagccctg gcaggcgatc ggcggcggtg agcagagccc
     1081 aggtcacgac cgtcacgacc gcgaagcggc gcagcacgat cgcgaacggg tggcgctcga
     1141 tccggtacga ccagctcagc aggccgatcg cgaccagccc gaggccgatc accgcggtct
     1201 gggtcagcca ccatgcattg accagcgcgt cggcggcgat gcagccgacc agcgtcagca
     1261 ccagggccca gccggccagc gcgatccacc ccgaccccgg caggcggcgc ggatgcaggc
     1321 acacggcgtt gaccatcgcc gctgcggtga acaggtcgaa gccggcgcgc accggcatgt
     1381 cccagcgcgc cagcggtgcc ggcaggccga agtcgaacac gcattccacc gcaatggcca
     1441 gcaggttgcc gcactggcac agcgccatca ccgcgtagat gaggttgcgg cccgacgggt
     1501 tcgccagcgg cacgaccatc gcgaccaggt acaggccgag cgcgaacgcc gacaacaccc
     1561 agaacaggat gggcaagccc agcaggccgc gcggcccgga ttgcacttcg gccaccgagc
     1621 cgtcggcgaa gaacagcgtg acctcgcggt gcgcggacac cgcggcgagt tgctcgagca
     1681 gcacgcgctg gcgcgcacgc tcgtcgtcgt cgatgaccca gcgcggcgag cgctgcaaca
     1741 ccggcgcgtc gctcaatacg accgtggcat cgccaccggt cgcgccgcgc agcacctgac
     1801 cgacctgcat gcgcagggcc gggtcgtcgc tcgcggcgag ctcgatgcgg ccctgtgcat
     1861 cgatgcgcca acgcgcgtcg atgtgcggcg ccagggccat cgcccggatc agcaggtaca
     1921 ggccgatgca gccaagcagc gcgaccgtga acagcaggcg caggcgccag cccatccagc
     1981 gcgcggccgg cagcgcttgc tcggcgccgc gttcaccgac gcccgaatcg agcccggagt
     2041 cgccgagctg gctggggtgg ccggtccagg tgggcgaggc gcggccgggc caggtgttgt
     2101 tcaggccggg cgtgcgcgca gggtcggtcg cgtcatgcac cacggccgcc tctcgcgagg
     2161 acctacacca ggccttgctt gctggcgagc acggccgctt cggcgcggct cgagacgttg
     2221 agcttcttgt agatggactt gatgtggtcg ttgacggtga accacttgat gcccatcagg
     2281 ttcgcgatct ccttgatcgt gaagcctttg ctcaggtagg tcagcacctc gctctcgcgc
     2341 ggcgtcagcc gttcgtggtc gagcggcgcg ccgcgcccca tcggcaccgg gcggctgctc
     2401 tggaagcccg agaccagcgg caccggcccc gagttgggcg cactgccgcc gccggggccg
     2461 tggcggaaat gggtcagcaa ccggcgcgcg atcgccggcg acagcggcgg ctggccgcgc
     2521 acgatcttct gcagctcctc gaccagcacc tcgaagcggt cttccttcag caggtagccg
     2581 tcggtgccgc actgcagcgc ggggaagaga tggtcgtcgt cggagtacag cgtcgtgacg
     2641 atcttggtgg ccgggtaggt gccgagctcg gtgagcagct ccatgccgtt gccgtcgggc
     2701 agctcgaggt cgaccaggat cagcttgaac gggtcggggg cctgcgcggc gtcgcgcagc
     2761 gggccgatct ggcggcgcgc ggtttcgagg tcgcgcgcct ccgtgatgat cagtgcgtca
     2821 ctgaaactct cgcgcacgac ccggctcaga aaactgcgcg cgaccgggtt gtcctcgacg
     2881 atgagtacct tgactggcat ctgctgcgcc cctcctcctt gtgtgtcggc gatggtagcg
     2941 caatcgacac ggcgcgattg cgcccgaaac gcggcttcag gggttggtca tcaccagctt
     3001 gccgcgcacc tggcggccgg ccatgacctc gaaagcctga agcagctgat ccatcggcag
     3061 ccgccggtcg atcaccggct tgaccaaccc ccgcgcatac cactgcgcga gttcggccag
     3121 cgccttcgcg ttcgccttcg gctcgcggcg catgaattcg ccccagaaca cgccgacgat
     3181 cgaggcgccc ttgagcaatg ccaggttcaa cggcagcgcc ggtatgccgc cctgcgcgaa
     3241 gcccacgacc aggtagcggc cgcgccacgc gatcgagcgg aacaccggct cggccaggtc
     3301 gccgccgacc gggtcgtaga ccacgtcggg gcccttgccg tcggtcagtg ccttgatctc
     3361 gtcgcggagg ttggccttgc tgtagttgat ggtcgcgtcg gcgcccagcg acttgcagag
     3421 ctcgcacttc gcatcgctcg atgccgccgc gatcacgcgc gcgccgaccg ccttcgcgat
     3481 ctggatcgct gcggtgccca cgccgccggc cgcgcccagg atcagcaccg tctcgccggg
     3541 cttcagcgcg gcgcggtcga gcagcgcgtg gtgcgtggtc gcgtaggtca gcacgaaggc
     3601 cgaggcgtcg tcgaacgcga agcccggcgg cagcggcatc acgagggcgg ccttcacgac
     3661 cgcatgcgtc gcgaagccgc cggtggcacc gcaggccgcg accgcgtcac cgaccttcag
     3721 ctgcgtgacg cccgcaccga ccgcctccac gacgccggcg tactccgagc ccggcacgaa
     3781 gggcagcggt ggcttgagct ggtacttgtt ctggacgatc aggatgtcgg ggaagttcag
     3841 gctcgcggcg cgaatcgcga cgcgcacctc gtc
//
LOCUS       DATMJB010000298         4781 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_11281, whole genome shotgun sequence.
ACCESSION   DATMJB010000298 DATMJB010000000
VERSION     DATMJB010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11281"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VNU48_06560"
     CDS             <1..311
                     /locus_tag="VNU48_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10973.1"
                     /translation="PAASARPPAPSGPRLRGPAETGRRAAAPGDLQPERPVAPQITIP
                     FGKKPPAPPTRNEPAPRSGAAPGGIGDAAARCESQVDPQELAACRARLAREGRAKLPN
                     "
     gene            377..2077
                     /locus_tag="VNU48_06565"
     CDS             377..2077
                     /locus_tag="VNU48_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HWI10974.1"
                     /translation="MALPRFTQLHPFRPRLLDALKGYTGARFGADLGAGITVGVVALP
                     LAMAFAIASGVKPEQGLFTAIIAGFLISALGGSQVQIGGPAGAFIVIIYGIIERYGLA
                     NLLIATSLAGVLMFVLGWFRLGALVRYVPLPIVIGFTNGIAVLIAVSQLRDLLGLQIT
                     GKLPGDFFALLHTLARHLASFNPYAFGLGVACLGGLFLWPHVFRPGGLLKRLALEQRL
                     GGRLRMASRMPGPIVALVSLSALSALLALPVETIGTRFGGIPAALPHFELPEFTWETA
                     KQLFIPTLTIALLGAVESLLCARVADNLIEAKRHDPNQELMAQGVANFVVPFFGGVPA
                     TGTIARTVTNVRAGATSPVAGIVHALVVLGVVLVAAPLAASVPLAVLAGILLFVAWNM
                     GEWNEFARLRNFSTQYRTILLGTFGLTVIFDLTVAVEVGLVLACVFFIYRMSTLFKVL
                     PHEPAPGEELPPGVQVFELYGSLFFGAVGGIEKLEAQVVPGTRALVLEMHRLILIDTS
                     GIDALQQLHRSLQRRGVAMVLANVNEQPLSLIRRSGLETIIGAEHIVPSLNELDAATR
                     "
     gene            2061..2924
                     /locus_tag="VNU48_06570"
     CDS             2061..2924
                     /locus_tag="VNU48_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HWI10975.1"
                     /translation="MRRPADSIVIDLDAYLQRIGYTGPREPTLAVLGAICSAHPAALA
                     FENIDPLLGRAPLLAPAALQAKLVAQRRGGYCYEHNALLRLALLALGMKVRSLAARVV
                     WMMPADAPRRARSHMLLMVELPEAPGESFLVDAGFGGHLLGAPLRFVPGLVQHTPGGT
                     ERITQDADTFTLEAALPEGWVPAYRFTLEPHAPVDYEPLNWFTATHPASIFRHNLRLE
                     RLTPTLRAGLLNDRLTLRPVGGATQVRRLESADELAQVLDEVFDLQPPVPATALFERV
                     PKGLDGAFVPV"
     gene            complement(2929..3648)
                     /locus_tag="VNU48_06575"
     CDS             complement(2929..3648)
                     /locus_tag="VNU48_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWI10976.1"
                     /translation="MDAALAASALLMGLAGAPHCAAMCGAAYAGLAGRHGARAAPRTL
                     AALLAGRLAGYAAAGALVAASVGAFADLAVAAPLLRPLWALTHVAAIALGVQLAWRGR
                     APAWLAGAGARARSALQAQPLRFVARVPAPARSVALGACWAAMPCGLLQSALIVAALA
                     SGPAQGAAVMAAFAVASAPGLWLAPALWRRLRRGSAGVAAPTVAVRLAGALLIGSSGF
                     ALVHGLRAAIDAALCIGTAAV"
     gene            complement(3648..>4781)
                     /gene="hemN"
                     /locus_tag="VNU48_06580"
     CDS             complement(3648..>4781)
                     /gene="hemN"
                     /locus_tag="VNU48_06580"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HWI10977.1"
                     /translation="AAPYLDALEVEIGLHAVELGVRQPVTQLHLGGGSPTFLTDAELT
                     RLMQMLRRAFAIAASAEISIEVDPRTADPQRLRHLADLGFNRLSLGVQDFDADVQAAV
                     HRVQSLDSVRNLLEAARTLGFHSTNVDLIYGLPRQTGASFERTVAQVCALRPDRIALY
                     AYAHLPQRFKPQRRIDAAALPTAAQKIGMLAQAIRGFAGAGYDYIGMDHFALPDDPLA
                     VARRQGRLHRNFQGYSTQPDCDLIGLGVSAIGRIGATYSQNAKTLPEYHDALAQHQFP
                     VARGLALTRDDLLRRAVIMALMCQGRVEFESIALAHLVDFRKAFATELAALQPLQAQG
                     LVVIDADAIEVTPLGRYFVRNVAMVFDRHLQADRARNHYSRVI"
BASE COUNT          620 a         1753 c         1750 g          658 t
ORIGIN      
        1 ggccggccgc atcggcgcgc ccgcctgcgc ccagcgggcc gcgtctgcgc ggcccggccg
       61 agaccggccg gcgggccgcg gccccgggtg acctgcagcc tgaacggccg gtcgcgccgc
      121 agatcaccat cccgttcggc aagaagccgc ccgctccacc gacgcgcaac gagccggccc
      181 cgcgcagcgg cgcggcgccg ggtggcatcg gcgacgcggc ggcgcgctgc gaatcgcagg
      241 tcgatccgca ggagctggcc gcgtgccgcg ccaggctggc gcgcgagggc cgcgccaagc
      301 tgccgaactg agtcgacgag ccggctcgcc ggccggggcg cctgccgcgg gacgtgcgac
      361 actgggcgac atttccatgg ccctgccccg cttcacgcag cttcacccgt tccgaccgcg
      421 cctgctcgac gccctgaagg gctacaccgg cgcgcgcttc ggtgccgacc tcggcgccgg
      481 catcaccgtc ggcgtggtcg cgctgccgct ggcgatggcg ttcgcgatcg cctccggcgt
      541 caagcccgag cagggcctgt tcaccgcgat cattgcgggc ttcctgatct cggcgctggg
      601 cggttcgcag gtgcagatcg gcggcccggc gggcgcgttc atcgtcatca tctacgggat
      661 catcgaacgc tacggcctgg cgaacctgct gatcgcgacc tcgctcgccg gcgtgctgat
      721 gttcgtgctc ggctggttcc gcctcggcgc gctggtgcgc tacgtgccgc tgccgatcgt
      781 gatcggcttc accaacggca tcgcggtgct gatcgcggtg tcgcaactgc gcgacctgct
      841 cggcctgcag atcacgggca agctgccggg cgacttcttc gcgctgctgc acacgctggc
      901 gcggcacctc gcgagcttca acccgtacgc gttcggcctc ggcgtcgcct gcctcggcgg
      961 gctgttcttg tggccgcacg tgttcaggcc gggcgggctg ctgaagcgcc tggcgctgga
     1021 gcagcgcctg ggcgggcggc tgcgcatggc ctcgcgcatg ccggggccga tcgtcgcgct
     1081 ggtgtcgctg agcgcgctca gcgcgctgct ggcgctgccg gtcgagacca tcggcacgcg
     1141 cttcggcggc atcccggcgg cgctgccgca cttcgagctg cccgagttca cctgggagac
     1201 ggccaagcag ctgttcatcc cgacgctgac gatcgcgctg ctcggcgcgg tcgagtcgct
     1261 gctgtgcgcg cgcgtcgccg acaacctgat cgaggccaag cgccacgacc cgaaccagga
     1321 gctgatggcg cagggcgtgg cgaacttcgt cgtgcccttc ttcggcggcg tcccggcgac
     1381 cggcacgatc gcgcgcaccg tcaccaacgt gcgcgccggc gcgaccagcc cggtcgccgg
     1441 catcgtgcat gcgctggtgg tgctcggcgt cgtgctggtg gccgcgccgc tggcggcgtc
     1501 ggtgccgctg gcggtgctcg ccggcatcct gctgttcgtg gcctggaaca tgggcgaatg
     1561 gaacgagttc gcgcggctgc gcaacttcag cacccagtac cgcacgatcc tgctcggcac
     1621 tttcgggctg acggtgatct tcgacctgac ggtggcggtc gaggtcggcc tggtgctggc
     1681 ctgcgtgttc ttcatctacc gcatgagcac gctgttcaag gtgctgccgc acgagccggc
     1741 gccgggcgag gagctgcccc cgggcgtgca ggtgttcgag ctctacggct cgctgttctt
     1801 cggggccgtc ggcgggatcg agaagctcga ggcgcaggtc gtgccgggca cccgcgcgct
     1861 ggtgctcgag atgcaccggc tgatcctgat cgacacctcg gggatcgacg cgctgcagca
     1921 gctgcaccgc agtctgcagc gccgcggggt ggcgatggtg ctggccaacg tcaacgagca
     1981 gccgctgtcg ctgatccgcc gctcggggct cgaaacgatc atcggcgcgg agcacatcgt
     2041 gccgagcctg aacgagctcg atgcggcgac ccgctgactc gatcgtgatc gacctcgacg
     2101 cctacctgca gcgcatcggc tacaccgggc cgcgcgagcc gacgctcgcg gtgctgggcg
     2161 cgatctgcag cgcgcacccg gcagcgctcg cgttcgagaa catcgacccg ctgctcggcc
     2221 gcgcgccgct gctcgcgcca gccgcattgc aggccaagct cgtcgcgcag cgccgcggcg
     2281 gctattgcta cgagcacaac gcgctgctgc ggctggcgct gctggcgctg ggcatgaagg
     2341 tccggtcgct ggccgcgcgc gtcgtctgga tgatgccggc cgacgcgcca cggcgcgcgc
     2401 gcagccacat gctgctgatg gtcgagttgc ccgaggcacc cggcgaatcg ttcctcgtcg
     2461 acgccggctt cggcggccac ctgctgggcg cgccgctgcg gttcgtgccc gggctggtgc
     2521 agcacacgcc gggcggcacc gaacgcatca cgcaggacgc cgacaccttc acgctcgagg
     2581 ccgcgttgcc cgagggctgg gtgccggcct accgcttcac gctggagccg catgcgccgg
     2641 tcgactacga accgctgaac tggttcaccg caacgcaccc ggcctcgatc ttccgccaca
     2701 acctgcggct cgaacggctg acgccgacat tgcgtgcggg gctgctgaac gatcgcctga
     2761 ccctgcggcc ggtgggcggc gccacgcagg tgcggcgcct cgaatcggcg gacgagctcg
     2821 cgcaagtgct ggacgaggtc ttcgatctgc agccgccggt gcccgccacc gcgctgttcg
     2881 agcgcgtgcc gaaggggctg gacggcgcgt tcgtgccggt ctgatgcgct acaccgccgc
     2941 ggtgccgatg cacagcgccg cgtcgatcgc cgcgcgcagg ccgtgcacca gcgcgaagcc
     3001 cgacgagccg atcagcagcg cccccgccag ccgcaccgcc acggtcggtg ctgcgacgcc
     3061 ggcgctgccg cgccgcaggc gacgccacag tgccggtgcc agccacaggc ccggcgccga
     3121 cgccaccgcg aaggccgcca tcacggccgc tccctgcgcc ggccccgacg ccagcgccgc
     3181 gacgatcaag gccgactgca gcaggccgca cggcatcgcc gcccagcagg cgccgagcgc
     3241 gacgctgcgc gccggtgccg gcacgcgcgc aacgaagcgc agcggttgcg cctgcagcgc
     3301 actgcgcgcg cgcgcgccgg cgcccgccag ccaggccggt gcgcggccgc gccacgcgag
     3361 ttgcacgccg agggcgatcg ccgcgacatg ggtcagcgcc cacaacggac gcagcagcgg
     3421 cgctgcgacg gccaggtcgg cgaaggcgcc gacgctcgcc gccaccagcg ccccggccgc
     3481 ggcgtagccc gcgagacggc cggccagcaa cgcggccagc gtgcgcggcg cggcgcgcgc
     3541 accgtggcga cccgccaggc cggcataggc ggcgccgcac atcgcggcgc agtgcggggc
     3601 gccggccagc cccatcagca gcgccgaggc ggcgagcgcg gcgtccatca gatcacgcgc
     3661 gagtagtggt tgcgggcgcg gtcggcctgc aggtggcggt cgaacaccat cgcgacgttg
     3721 cgcacgaagt agcggcccag cggcgtgacc tcgatcgcat cggcgtcgat caccaccagg
     3781 ccctgcgcct gcagtggctg cagcgccgcc agctcggtcg cgaacgcctt gcggaagtcc
     3841 accaggtgcg ccagcgcgat cgattcgaac tcgacccggc cctgacacat cagcgccatg
     3901 atcacggcgc gccgcagcag gtcgtcacgt gtcagcgcga ggccgcgggc gaccgggaac
     3961 tggtgctgcg cgagggcgtc gtgatactcc ggcagcgtct tcgcgttctg gctgtaggtc
     4021 gcgccgatgc ggccgatggc cgagacgccg aggccgatca ggtcgcagtc gggctgcgtg
     4081 ctgtagccct ggaagttgcg gtgcagccgg ccctggcgcc gggcgacggc gagcgggtcg
     4141 tcgggcagcg cgaagtggtc catgccgatg tagtcgtagc cggcgccggc gaagccgcgg
     4201 atcgcctgcg ccagcatgcc gatcttctgc gcggccgtcg gcagcgccgc cgcatcgatg
     4261 cgccgctgcg gcttgaagcg ctgcggcagg tgcgcgtagg cgtacagcgc gatccggtcg
     4321 gggcgcagcg cgcagacctg cgcgacggtg cgttcgaacg acgcgccggt ctggcgcggc
     4381 aggccgtaga tcaggtcgac gttggtcgag tggaaaccca gcgtgcgcgc cgcctcgagc
     4441 aggttgcgca cgctgtccag gctctgcacc cggtgcaccg ccgcctgcac gtcggcatcg
     4501 aagtcctgca cgcccaggct caggcggttg aagccgagat cggcgaggtg gcgcaggcgc
     4561 tgcggatcgg cggtgcgcgg atcgacctcg atcgagatct cggcgctcgc ggcgatcgcg
     4621 aacgcacgcc gcagcatctg catcagccgc gtcagctcgg cgtcggtcag gaaggtcggc
     4681 gagccgccgc ccaggtgcag ctgcgtgacc ggctggcgca ccccgagttc gaccgcgtgc
     4741 aggccgatct cgacctcgag cgcgtcgagg tagggcgcgg c
//
LOCUS       DATMJB010000299         2273 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_702677, whole genome shotgun sequence.
ACCESSION   DATMJB010000299 DATMJB010000000
VERSION     DATMJB010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702677"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..912)
                     /locus_tag="VNU48_06585"
     CDS             complement(112..912)
                     /locus_tag="VNU48_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI10978.1"
                     /translation="MIRIEQLAKSFIATGGGPMSGLMPKRRSPHVVHAVRDVNLHAPD
                     GHITGLLGPNGAGKTTSLRMLAGLIAPDAGRIHVDGIDVRTRPRAALARMGVLSDARG
                     LYPRLTARENIVYYGRLQGMERDAAHARAEALAQMFEMGSLLERRTDGFSQGERMKTA
                     LARALVHDPANIVLDEPTNGLDVLATRALRDSLRWLRTPEGGAKCIVFSTHIMQEVER
                     LCDSVVVVAHGRTVAAGTVGELLAQTRERDFEEAFVKLAFVDELARVE"
     gene            complement(909..>2273)
                     /locus_tag="VNU48_06590"
     CDS             complement(909..>2273)
                     /locus_tag="VNU48_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI10979.1"
                     /translation="SVQCGVVQRALDLAQPEGPKIAVHYVVVPAMARRKLADPVFLLA
                     GGPGQSAIDIAPQLMPLFARLNNRRDIVFVDQRGTGRSAPLDCAEAQRETLAEQSDTE
                     RQVALLMRCKTELMQRPALRGPGALGFFTTTIAMGDLDAVRARLGVQRIDLVGVSYGT
                     RAALEYMRQFPAAVRRSVLDSVAPPDMALPASFSTDAQAALDALLAACEREAECRRAH
                     PRLRADWAALLKSLPKAVVAPQPLSGTPERFTLTRDMVLAAVRGALYNPAVAAALPAA
                     ISAAAAGRYEALTGIGSLLGSRRGMRLAAGMHFSVVCAEDLPLLDKAIDPPGGDFGDS
                     QARLYRRVCADWPRGAVPSAFYTIPASRTPTLLLSGGLDPATPPRHGARVAQALGAAA
                     RQVVVANAGHSVLGIGCARDLLFRFIDAADDAAAAAVDAGCLKNIPRPGVFRPIAGTV
                     EGSK"
BASE COUNT          303 a          829 c          843 g          298 t
ORIGIN      
        1 ccgccggccc catcagcacc gacgagacga gcaccgtcag cagcgtgcgc cggtcgcgca
       61 gcgcgtcgcg gagctccttg cgcaagatcg tcagcagcgt ctgcaggcgg ctcattccac
      121 acgcgccaat tcgtcgacga aggcgagctt cacgaaggcc tcctcgaagt cccgctcccg
      181 cgtctgcgcg agcagttcgc cgaccgtgcc ggccgcgacc gtgcggccgt gcgcgaccac
      241 gacgacgctg tcgcacagcc gctcgacctc ctgcatgatg tgggtcgaga agacgatgca
      301 cttcgcgccg ccctcgggcg tgcgcagcca gcgcaggctg tcgcgcagcg cgcgggtggc
      361 gagcacgtcc agcccgttgg tcggctcgtc gagcacgatg ttggcggggt cgtggacgag
      421 cgcgcgcgcc agcgccgtct tcatccgctc gccctggctg aagccgtcgg tgcgccgctc
      481 gagcagcgac cccatctcga acatctgcgc cagcgcctcg gcgcgcgcgt gggcggcgtc
      541 gcgttccatg ccttgcaggc ggccgtagta gacgatgttc tcgcgtgccg tcagcctcgg
      601 gtacaggccg cgcgcgtccg acagcacgcc catgcgcgcg agcgcggcgc gcggccgcgt
      661 gcgcacgtcg atgccgtcca cgtggatgcg gccggcgtcg ggcgcgatca gcccggcgag
      721 catgcgcagg ctcgtcgtct tgccggcgcc gttcggtccg agcagcccgg tgatgtggcc
      781 gtcgggcgcg tgcaggttca cgtcccggac cgcgtgcacg acgtgcggcg accggcgctt
      841 cggcatcaag ccactcatcg gcccgccgcc ggtcgcaatg aacgacttcg ccagctgctc
      901 gatgcggatc acttcgagcc ttccacggtg ccggcgatcg ggcggaacac gccggggcgc
      961 gggatgttct tcaggcagcc cgcgtcgacc gcggcggcgg ccgcatcgtc ggccgcgtcg
     1021 atgaagcgga acagcaggtc gcgcgcgcag ccgatcccca gcacgctgtg gccggcattg
     1081 gccacgacca cctggcgtgc cgccgcgccg agcgcctggg cgacgcgcgc gccgtggcgc
     1141 ggtggcgtcg ccggatcgag cccgccgctg agcagcagcg tcggcgtgcg cgacgccgga
     1201 atcgtgtaga acgccgacgg caccgcgccg cgcggccagt cggcgcagac acggcggtac
     1261 agccgcgcct ggctgtcgcc gaagtcgccg ccgggcgggt cgatggcctt gtcgagcagc
     1321 ggcaggtcct cggcgcagac gaccgagaag tgcatgcccg ccgccagccg catgccgcgc
     1381 cgtgagccga gcagcgagcc gatgccggtg agtgcctcgt agcggccggc cgcggcggcg
     1441 ctgatcgccg cgggcagcgc cgccgccacc gccgggttgt agagtgcgcc gcgcaccgcg
     1501 gcgagcacca tgtcgcgggt cagcgtgaag cgctcgggcg tgccgctcag cggctgcggc
     1561 gcaacgaccg ctttcggcag gctcttcaac agcgcggccc agtcggcgcg caagcgggga
     1621 tgcgcgcggc ggcattcggc ctcgcgctcg caggcggcga gcagcgcgtc gagcgcggcc
     1681 tgagcgtcgg tcgagaagct cgcgggcagc gccatgtcgg gcggcgcgac cgagtcgagc
     1741 acgctgcgtc gcaccgcggc cgggaactgg cgcatgtact cgagcgcggc acgcgtgccg
     1801 tacgagacgc cgaccaggtc gatgcgctgc acgccgagcc gggcccgcac cgcatcgagg
     1861 tcgcccatcg cgatcgtcgt cgtgaagaac ccgagcgcgc cgggcccgcg cagcgcgggt
     1921 cgctgcatca gctcggtctt gcaacgcatc agcagcgcga cctggcgttc ggtgtccgat
     1981 tgctcggcga gggtttcgcg ctgggcctcg gcgcagtcca gcggcgcact gcggccggtg
     2041 ccgcgctggt cgacgaagac gatgtcgcgc cggttgttca ggcgcgcgaa cagcggcatc
     2101 aactgcggcg cgatgtcgat cgcgctctgg cccggcccgc cggcgagcag gaacaccgga
     2161 tcggccagct tgcgccgcgc catcgccggc acgacgacat agtggacggc gatcttcggg
     2221 ccttcgggtt gcgccaggtc gagggcgcgc tgcacgacgc cgcactggac gct
//
LOCUS       DATMJB010000300         3085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_121274, whole genome shotgun sequence.
ACCESSION   DATMJB010000300 DATMJB010000000
VERSION     DATMJB010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121274"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1775
                     /locus_tag="VNU48_06595"
     CDS             201..1775
                     /locus_tag="VNU48_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWI10980.1"
                     /translation="MTTSISLPLTTALVSARDQRRVGFAVALLCVGQVLLWSVMAGLN
                     YTSPEIDSAEQFVWAFSMENGYWKHPPMPSWIMHGLLQAFGPSVELPFVATQVCIVTA
                     LALTWRLGREFMSPARSLIAMALTSLVTYHNIGGDSFNHNTVLLPFQAATVLLFYLAC
                     RRQAWHLWMLVGAFAGLSMLVKYVALLPLAGLLVYFALDRSLHTRRACSGLLLAVAVL
                     ALVLLPHFLWLRATNFLPFRYAHGVTQELPGVLAALRSLADFGLTQVLRLLPFLIGLG
                     LVLRAPAAPDAVLPAAPIAAADRLFVWVVTCAPLVITMGVGLVTETALQARWGTNGFL
                     FAGLLVMTLLRRPDDPAMLRRCLQLVVAIQVLLCAGQTLTKTVVADHLERRTRANFPG
                     ATLAELARQTWAAHTDAPLRLVVSDIWLGGNIIANSPKRVAVLIDGHYYKSPWVRREA
                     VERCGALILDDTTGDQAGHAEPDPAIDTLMARAEFTGTWTLPWAHPKLRADSPERGVV
                     RWGIIRPHSAESCSLH"
     gene            complement(1788..>3085)
                     /locus_tag="VNU48_06600"
     CDS             complement(1788..>3085)
                     /locus_tag="VNU48_06600"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HWI10981.1"
                     /translation="DSRLSYGFVAGPGTFETTLTRPDLFANYYLEQFRLLLKNHRVSI
                     EIGTSAQPIPVHFSFAENDHIEGSMSPERRTLMRDRFDLPDLNAMDDGIANGTHEPAP
                     GAAQPLALFTAPRVDYSLHRLRHYTGTAPEHFQNFVLFTNYQFYIDEFVRLGHETMTS
                     TAKAQDYVAFVEPGNVVTRRNGLAALPGDEFGAAPPRPPQMPAYHLVRADGGGITMVN
                     IGVGPANAKTITDHIAVLRPHAWVMLGHCAGLRNTQQLGDYVLAHGYVREDHVLDEEL
                     PLWVPIPALAEVQVALEAAVEQITQLKGYELKRIMRTGTVASTDNRNWELLPSHHAAS
                     TPERRFSQSRAIALDMESATIAANGFRFRVPYGTLLCVSDKPLHGEIKLPGMANHFYR
                     ERVDQHLRIGIRAIELLREAGVDQLHSRKLRSFAEVAFQ"
BASE COUNT          418 a         1025 c         1078 g          564 t
ORIGIN      
        1 gtgacggggg gcttgcttgg tgtgagaaac agcgtaaggg atagcggggc gccgggcaat
       61 tgggggtctg cgcaacacgg cagcagccta caatcctcaa tacacatttc ctaacgaaac
      121 gtgccgcgcg cgcggaccgc cgaacccaat atgcccaatg ctgcggacgc gcgtttcctc
      181 agcttcgggc ccgccctccc gtgacgactt cgatcagcct gcctttgacg acggcactgg
      241 tgtccgctcg cgatcaacgc cgagttggct tcgcggtcgc actgctgtgc gtcggtcagg
      301 tgctgttgtg gagcgtgatg gccgggttga actacacctc gccagagatc gacagcgccg
      361 agcagttcgt ctgggcgttt tcgatggaga acggctactg gaagcacccg ccgatgccct
      421 cgtggatcat gcacggcctg cttcaggcgt ttgggccgtc ggtcgaactg ccgttcgtgg
      481 cgacacaggt ctgcatcgtg accgcgctgg cgttgacctg gcggctgggc cgcgagttca
      541 tgtcgcccgc ccgttcgctg atcgcgatgg cgctgacctc gctcgtcacg tatcacaaca
      601 tcggcggcga cagcttcaac cacaacaccg tgctgctgcc gttccaggcc gcgacggtgc
      661 tgctcttcta cctcgcgtgc cggcgccagg cctggcatct gtggatgctg gtcggggcgt
      721 tcgccggcct gtcgatgctg gtcaagtacg tggcgctgct gccgctggcg ggcttgctcg
      781 tctacttcgc cctcgatcgc agcctgcaca cgcggcgtgc gtgcagcggc ctgctgctgg
      841 ccgtggcggt gctcgcgctc gtgctgctgc cgcacttcct gtggctgcgt gcgaccaact
      901 tcctgccgtt ccgctacgcc catggggtga cgcaggagct gcccggagtg ctcgcggcgc
      961 tgcgcagcct ggcagacttt ggcctgaccc aggtgctgcg gctgttgccg ttcctgatcg
     1021 gtctcggtct ggtgctgcgc gcgccagcgg cgccggacgc tgtcctgcct gcggcgccga
     1081 ttgccgccgc cgaccgtctg ttcgtctggg tggtcacctg cgcgccgctg gtgatcacga
     1141 tgggcgtcgg cctggtcacc gagaccgcgc tgcaggcgcg ctggggtacg aacggcttcc
     1201 tgttcgccgg tctgctggtg atgacgctcc tgcgccggcc tgacgatccg gcgatgctgc
     1261 gacgctgcct gcagttggtg gtggcgatcc aggtattgct gtgcgcgggt cagaccctga
     1321 ccaagacggt tgtggctgac cacctcgagc gccgcacgcg cgccaatttt cccggcgcca
     1381 cgctggccga gctggcgcgc cagacctggg ccgcacacac cgacgcgccg ctgcgtctgg
     1441 tggtttccga catctggctc ggtggcaaca tcattgcgaa cagcccgaag cgcgtcgcgg
     1501 tgctaatcga cgggcactac tacaagtcgc cctgggttcg gcgtgaggca gtcgagcgct
     1561 gcggcgcgct gatcctcgac gacaccaccg gggaccaggc cggccacgcc gagccggacc
     1621 ctgcgatcga cacgctcatg gcgcgcgccg agttcaccgg cacatggacg ctgccctggg
     1681 cccaccccaa gctgcgtgcc gactcgcctg agcgcggcgt ggtgcgctgg ggcatcatcc
     1741 ggccccactc cgccgagtcc tgctccctac actgagggcc cgacgcgcta ctgaaacgcc
     1801 acttccgcga agctgcgcag cttgcggctg tgcaactggt cgacgccggc ttcgcgcagc
     1861 agctcgatcg cgcggatgcc gatgcgcaag tgctggtcga cgcgctcgcg gtagaagtgg
     1921 ttcgccatgc ccggcagctt gatctcgccg tgcagcggct tgtcgctgac gcacagcagg
     1981 gtgccgtagg gcacgcggaa gcggaagccg ttcgccgcga tcgtcgctga ctccatgtcg
     2041 agcgcgatcg cgcggctctg gctgaagcgg cgctccggcg tgctcgccgc gtggtgcgag
     2101 ggcagcaact cccagttgcg gttgtcggtg ctcgccaccg tgccggtgcg catgatgcgc
     2161 ttgagctcgt agcccttgag ctgcgtgatc tgctcgaccg ccgcctcgag cgcgacctgc
     2221 acctcggcca gcgccgggat cggcacccac agcggcagct cctcgtcgag cacgtggtcc
     2281 tcgcgcacgt agccgtgggc gagcacgtag tcgccgagct gctgcgtgtt gcgcaggccg
     2341 gcgcagtggc cgagcatcac ccacgcatgc gggcgcagca ccgcgatgtg gtcggtgatc
     2401 gtcttcgcgt tggccgggcc gacgccgatg ttgaccatcg tgatgccgcc gccgtcggcg
     2461 cgcaccaggt ggtaggccgg catttgcggc gggcgcggcg gcgccgcgcc gaattcgtcg
     2521 cccggcagcg cggccagccc gttgcggcgc gtgaccacgt tgccgggttc gacgaaggcg
     2581 acgtagtcct gtgccttggc ggtactggtc atcgtctcgt gcccgaggcg cacgaactcg
     2641 tcgatgtaga actggtagtt cgtgaacagc acgaagttct ggaagtgttc gggcgcggtg
     2701 ccggtgtagt ggcgcaatcg gtgcagcgaa tagtccaccc gtggcgccgt gaacagcgcc
     2761 agcggctgcg ccgcaccggg cgccggctcg tgggtgccgt tggcgatgcc gtcgtccatc
     2821 gcgttcaggt cgggcaggtc gaagcggtcg cgcatgaggg tgcgacgttc ggggctcatg
     2881 ctcccttcga tgtgatcgtt ttcggcgaac gagaagtgca cggggatcgg ctgcgcactg
     2941 gtgccgatct cgatcgacac ccggtggttc ttcagcagca ggcggaactg ctcgaggtag
     3001 tagttcgcga acaggtccgg ccgcgtcagc gtggtctcga aggtgccggg gccggcgacg
     3061 aaaccgtagc tcaggcgcga gtccg
//
LOCUS       DATMJB010000301         3072 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_860669, whole genome shotgun sequence.
ACCESSION   DATMJB010000301 DATMJB010000000
VERSION     DATMJB010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860669"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1537
                     /locus_tag="VNU48_06605"
     CDS             <1..1537
                     /locus_tag="VNU48_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI10982.1"
                     /translation="IGLFVVRGLAGFAVDYTLAWSANRATLTLRTELFGRLLDANPRL
                     FAERSASSLMNTVVYEILGGVNQLVGAVQTLLKDSFTVIFLLGTLLVLNWRLTLFIAA
                     IGPAVGYTMRVFGRRMHRITKESQLAVDRLGYVVEENVMAWRMVRLHGVQDVQRTRFE
                     RSSAALRRLLMKSTVASATVTPITQLLTVCAIAAVIAFALWQAKPDAGMGPFVMFITA
                     AIAVATPLRRLTDVSAPISRGIASVERGLDLIHHAPRETGGSHAPGRARGDIALQGVT
                     VRFGSAGTAPALDAVDLQIRAGETIALVGPSGAGKTTLVNLLPRFLDPCSGSITLDNV
                     ALPAWDLQALRDQFALVSQDVVLFNDTVAANVCFGAPADAGLVRAALVAANLGGFVDD
                     LPLGVDTMIGHNGTQLSGGQRQRLAIARAIYKDAPILILDEATSALDSESERLVQQAI
                     ETLMKGRTSLVIAHRLSTIERADRIIALEAGRVVEQGSHAELLSHGGLYARLHALQFR
                     SST"
     gene            1584..2525
                     /locus_tag="VNU48_06610"
     CDS             1584..2525
                     /locus_tag="VNU48_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10983.1"
                     /translation="MKRVFVTDYVGLSTRLEALALAFMISDYFGHEVCIDWNELDALH
                     VPGANIRGRGLLGRLDSLKLHGDASSDLHRAADHRNVNVRTHHGPRHLLEKYYLPTAR
                     RVKLRPDLIEMIRGTFARYSDRPLVGVHIRRGDFLMASDTEFDVNALQWPAVPNWWYE
                     HVMAQIQQAVPDVAFFVSCTGSLDQFPLLQARFEMFDMPAASPYKYTHHRKAGHLSHR
                     HPAVDLFALGCCTTLIGTPCSTFTHYAANMLGAPTTVLIPPAHKIQADAPEYGRVELH
                     GRGAADWYATCRTGRGQVAVSDAATLSIAHGAFVEWM"
     gene            2531..>3072
                     /locus_tag="VNU48_06615"
     CDS             2531..>3072
                     /locus_tag="VNU48_06615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWI10984.1"
                     /translation="MNYTVVIPVLNQLHYTEQCVASLLASGVPADALLVIDNGSTDGT
                     PGWLAARSAIRSVRNPVNLGCGGAWTQGALLSDRDWVVLLNNDIVCAHNFIEAQIRSA
                     EQHGLEVVSPAMVEFELDYDLAQFTDEFLAKMSGTVREGWFHGVCFSVKREVFERIGF
                     LDTDRLLFGREDAEFLARCR"
BASE COUNT          480 a         1077 c         1005 g          510 t
ORIGIN      
        1 gatcggcctg ttcgtcgtgc gcggcctggc cggcttcgcc gtcgactaca cgctcgcctg
       61 gtcggcaaac cgcgcgacgc tgacgctgcg taccgagctc ttcggccgcc tgctcgacgc
      121 gaacccgagg ctgttcgccg agcgttccgc cagcagcctg atgaacacgg tggtctacga
      181 aatcctgggc ggcgtgaacc agctcgtcgg cgcggtacag acccttttga aggacagctt
      241 tacggtcatc ttcctgctcg gcaccctgct cgtgctgaac tggcgcctca cgctgttcat
      301 cgccgcgatc ggcccggccg tgggctacac gatgcgcgtc ttcggccggc gcatgcaccg
      361 catcacgaag gagtcgcagc tggcggtcga tcggctcggc tacgtcgtcg aagaaaacgt
      421 gatggcctgg cgcatggtgc ggctgcatgg cgtgcaggac gtgcagcgca cgcgcttcga
      481 gagatcgagc gccgcgctgc gccgcttgct gatgaaatcg accgtggcct cggccaccgt
      541 cacgccgatc acgcagttgc tgaccgtctg cgcgatcgcc gccgtgatcg cgttcgcact
      601 gtggcaggcc aagcccgacg cgggcatggg cccgttcgtg atgttcatca ccgcggcgat
      661 cgcggtcgcg acgccgctgc gccggctcac cgacgtgtcg gcgccgatct cgcgcgggat
      721 cgcctcggtc gagcgtgggc tcgacctgat ccaccacgcg ccgcgcgaga ccggcggcag
      781 ccacgcgccc ggccgcgccc gtggcgatat tgcgctgcaa ggcgtgacgg tgcgcttcgg
      841 ttccgccggc accgcgccag ctcttgacgc cgtggacctg caaatccgtg cgggcgagac
      901 gatcgcgctg gtcggtccgt cgggcgcggg caagacgacg ctggtgaacc tgctgcctcg
      961 gttcctcgac ccttgctcgg gttcgatcac cctcgacaac gtggcgctgc cggcgtggga
     1021 cctgcaggcg ctgcgcgacc agttcgccct ggtcagccag gacgtggtgc tgttcaacga
     1081 cacggtcgcg gccaacgtgt gcttcggcgc gccggccgac gccgggctcg tccgcgctgc
     1141 gctggtcgct gcgaatctgg gcggtttcgt cgacgacctg ccgctgggcg tggacacgat
     1201 gatcggtcac aacggcacgc agctctcggg cggccagcgc cagcgcctcg cgatcgcgcg
     1261 tgcaatctac aaggacgcgc cgatcctgat cctcgacgag gccacctcgg cgctcgactc
     1321 cgaatccgag cgcctcgtgc agcaggccat cgagacgctg atgaagggtc gcaccagcct
     1381 cgtgatcgcg caccgcctct cgaccatcga gcgcgccgac cgcatcatcg cgctcgaggc
     1441 cggccgcgtc gtcgaacagg gcagccacgc cgaactgctg tcgcacggcg ggctgtacgc
     1501 gcggctgcac gccctgcaat tcaggagttc cacatgagca agccagtcac gacgacaccc
     1561 cgtcgcggcg ccacgccaac aacatgaaaa gagtgttcgt cacggactat gtcgggctca
     1621 gcacgcgact cgaagcgctg gcgctggcgt tcatgatttc ggactacttc ggacatgagg
     1681 tctgcatcga ctggaacgag ctcgacgcgc tgcacgtccc gggtgcgaac atccgcggcc
     1741 gcggcctgct cggtcggctg gacagcctga agctgcacgg cgacgcgagc agcgatctgc
     1801 accgagcggc ggatcatcgc aacgtcaacg tgcgcaccca ccatgggcca cgccacctgc
     1861 tcgagaagta ctacctgccg acggcgcggc gggtgaagct gcgcccggac ctgatcgaga
     1921 tgatccgggg cacgttcgcg cgctattccg atcggccttt ggtcggcgtc cacatccggc
     1981 gcggcgactt cctgatggcc agcgacaccg agttcgacgt caacgcgctc caatggcccg
     2041 cggtgcccaa ctggtggtac gagcatgtca tggcgcagat ccagcaggcg gttccggatg
     2101 tggcgttctt cgtctcgtgc accggcagcc tcgatcagtt tccactcctg caggcgcgct
     2161 tcgagatgtt cgacatgccg gccgcgtcgc cctacaaata cacgcaccac cgcaaggccg
     2221 ggcacctgtc acatcggcat ccggcggtcg acctgttcgc gttggggtgc tgcacaaccc
     2281 tcatcggcac gccctgctcg accttcaccc actacgccgc caacatgctg ggcgcgccca
     2341 ccacggtgct gataccgccg gcccacaaga ttcaggccga cgcgcccgag tacgggcggg
     2401 tcgagctgca cggccgtggc gcggccgact ggtacgcgac gtgccgcacc ggccgcggcc
     2461 aggtcgcggt cagcgacgcc gccaccttgt cgatcgcgca cggcgccttc gtcgaatgga
     2521 tgtgagcgca atgaactaca ccgtcgtgat cccggtgctg aaccagctgc actacaccga
     2581 gcaatgcgtc gccagcctgc tcgccagcgg tgtcccggcc gatgccctgc tcgtgatcga
     2641 caacggcagc accgacggta cgcccgggtg gttggccgcg cgcagcgcca tccgctcggt
     2701 gcgcaacccg gtgaacctcg gctgcggcgg cgcctggacc caaggtgcac tgctgtcgga
     2761 tcgcgactgg gtcgtgctgc tcaacaacga catcgtctgc gcccacaatt tcatcgaggc
     2821 acagatccgt tccgcggagc agcatgggct cgaggtcgtg agcccggcga tggtcgagtt
     2881 cgaactcgac tacgacctgg ctcaattcac cgacgaattt ctcgccaaga tgagcggcac
     2941 cgtgcgcgag ggctggtttc acggcgtgtg cttctcggtc aagcgggaag tgttcgagcg
     3001 catcggcttt ctcgacaccg accggctgct gttcggccgg gaggatgccg agttcctggc
     3061 gcgctgccgc ag
//
LOCUS       DATMJB010000302         4916 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_186793, whole genome shotgun sequence.
ACCESSION   DATMJB010000302 DATMJB010000000
VERSION     DATMJB010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186793"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1571..3073)
                     /locus_tag="VNU48_06620"
     CDS             complement(1571..3073)
                     /locus_tag="VNU48_06620"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HWI10985.1"
                     /translation="MVAPDNSKDSTATPPAPAPAEPKERLVTTEHRLFVGKKTLEYTA
                     TCGTVVLRDYIPSYDPKDTNPKKPDKATASFFFVAYTLKTKKPEQRPVTFSFNGGPGS
                     SSVWLHLGILGPQRVACDEFGHAPPPPYALVDNEHTLLTDSDLVFIDPVGTGHSRMAE
                     GEKPNEFHEYQRDLDSVGEFIRMYLTRFGRWGSPKFLIGESYGTTRAAGLSLHLQGKH
                     DISLNGVMLVSMAVDLQTLSFDEGNELPYALFLPTYAATAWYHRALVPEWQKRPLAEL
                     VAAAEAFALGDYNAALLQGSRLAPAQREQVAAQLARFTGLTPEYVKRADLRPEAFRYS
                     KELLRTRGQTVGRLDSRFIGQDRDDAGEHPETDPFMNAVLGAYAVGINRVLRDALKYE
                     VDAPYVLHAPIWDKWTWKGFENRYVNIGSSLRKAMQANPHMRVYVASGYYDLGTPHAA
                     GDYTLSHLGLRGAAQAKVSVSYFEAGHMMYIHAPSLARMAKELRAFVRGG"
     gene            3129..3974
                     /locus_tag="VNU48_06625"
     CDS             3129..3974
                     /locus_tag="VNU48_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HWI10986.1"
                     /translation="MTGAPRRFPALAGVDFTSRPTRRKPITVALGHVQHGALRLDRIE
                     SHADFDSFARWLQTPGPWLGVFDLPFGLPRELVTTLRWPLQWEALMRHYAALSRAEIR
                     ATFAAFCAARPAGAKFAHRAADGPAGSSPAMKWVNPPVAFMLHAGVPLLLAAGVHLPG
                     LHDGDAMRVALEGYPGLLAREVLGARSYKSDTKARQTSERLIARIDLVDALDQGRTRL
                     ALRLKLTHAQREALISDASGDRLDAVLCLMQAAWASLRPNHGLPPTIDPLEGWIVTAG
                     TELGA"
     gene            4000..>4916
                     /locus_tag="VNU48_06630"
     CDS             4000..>4916
                     /locus_tag="VNU48_06630"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HWI10987.1"
                     /translation="MQAFNLLLLAGGLLVFISLLAGVLSTRLGLPFLLVFLVAGMLVG
                     VDGPVGVRFDNAQLSAWVGNAALAMILLEGGISTRMQTFRAGFRPALLLATLGVALTA
                     AMVGAVAMSAMHLDWRHGLLLGAIVGSTDAAAVFSLLRQTGVRLSERVSATLELESGL
                     NDPMAVFLVLALIATLKTDGGVADVAWLFVRQAGFGAAFGLLGGALVAWLLARLPLNV
                     EHGGVLALIVVSAGMAVFAAAGLVEGSGFLAIYLFGLRLRARAEPAAHAASSALDGFA
                     WAAQATMFLLLGLLVSPHDMLLTLAPTLAV"
BASE COUNT          660 a         1653 c         1769 g          834 t
ORIGIN      
        1 gacacgcaaa cggttcgccg cgatcgatcg aacacgaggt tcaaacgagt ctcgtacagg
       61 ggcttctcac ttcggctgcg ggtcacggtc gcgccactga tctcgatgcg atcacccacg
      121 cggcgcatcg tgcgcacgcc caccacgctg ccctcggtca gcgtcttgac gcggccgatg
      181 ccgttgccgg cgtgccattc gaacaagtcg tcccgatcgg tgggtccgaa ggtcagcgac
      241 gggatgtccc tgcccaggta cgaccagcag aaggagaaga aggtgtcgcg cgtgcgccag
      301 aagcagaact gcccttccgg gctgacatgc gcgaactccg catcgatggc gggcatctgc
      361 acgcgttggc cgtgccgcgg cgcggcttcg ttcaagcgct cccaggcgcg ggtcagcgcg
      421 atgcatgcga aggtgtcggt ttcgtacttg ccgagctggc cgccgaactg ccccgcggtg
      481 aagcgacggc cgtagaagct gccgtcctcg ttgcctgccg cctcggcggc gagaaacgac
      541 acccgctggc gctggatgcg tcgaatctcg ggatcgacga tgcagtcatc gaggtaggac
      601 agcgcgggca cgatgaagta ctcgccgtag gtgtaccggg cccagtcctg gccgccgatg
      661 taggcgaagc ggtgggtgag gaaggtgttc ctgaccaggc accacatgtc gtcgacgtga
      721 tgctgcagcg cctgcggcac ctcgatgccg gccagtctca gggcatggaa gcagtaggcc
      781 ttcgacagca gcgggctcgc cacatagcag aagtgatagc tgccgtggtt ctcgagcgag
      841 aagtcgccgt gcacgttgca ggtgtacacc gcgtcggcga cgcgttgccc gccgacggtc
      901 tgcgtcgact gccgatcctg aggcaccgag aacacgttga acgagaactc gatcagcttg
      961 tcgacccatt gcgccgcgtg cgcgtggccg ggcgccagcg ccagcgcgac cgcgagcact
     1021 tcgcagtccc atgcggtttc ctcggccttg gtgtcgagga acaggccggt gggcgcgtgg
     1081 cgctgcagtt gccgatctgc ttctgcggtc acgacccgca tgatgttcgc ctgcagttcc
     1141 tcgtccggcg ggcttcccat ctgcaggacg gcgaggccca gcttcgcggc ccaccaggca
     1201 gactgccact ccagccccca cgtgccaccg tctgcgcagg tcgacgtgcc ggtgcgatgc
     1261 gaatcgcaca ggtagttcat gacggccacg acctggtcgc gcagccgcgc gttcgcgtcg
     1321 gaatccggcg cgtcctggtc gcgaagatag accgccgccg cgaacacgac attggcgttg
     1381 gatcggatcc cgttctctcc cgacgatccg tcgccgaaat aggcccggcg cgaatcgacc
     1441 gcgtgccacg cgttcgagag gtaacgggga agtctgccaa gaagctccgc gctggcgcgc
     1501 gcgagctggg agttcggatt catcggtgtc cgttggtcgc ggggtgccaa gtgctgatcg
     1561 cgcgcggggc tcagccgccc cgcacgaacg cgcgcagctc cttcgccatg cgcgccagcg
     1621 acggcgcgtg gatgtacatc atgtggccgg cctcgaagta gctgacgctg accttggcct
     1681 gcgctgcccc gcgcagcccg aggtggctga gcgtgtagtc gccggccgcg tgcggcgtgc
     1741 ccaggtcgta gtagccgctg gcgacgtaga cgcgcatgtg cgggttcgcc tgcatcgcct
     1801 tgcgcaggct cgacccgatg ttgacgtacc ggttctcgaa acccttccag gtccacttgt
     1861 cccagatcgg cgcgtgcagc acgtagggcg cgtcgacttc gtacttcagc gcgtcgcgca
     1921 gcacgcggtt gatgccgacg gcgtaggcgc cgagcaccgc gttcatgaac gggtcggtct
     1981 cggggtgctc gccggcgtcg tcgcgatcct ggccgatgaa gcgcgagtcc aggcgcccga
     2041 ccgtctggcc gcgcgtgcgc agcagttcct tggagtagcg gaacgcttcg gggcgcaggt
     2101 cggcacgctt cacgtactcg ggcgtgaggc cggtgaagcg cgcgagctgc gcggcgacct
     2161 gttcgcgctg cgccggtgcg aggcgcgagc cctgcagcag cgcggcgttg tagtcgccca
     2221 gcgcgaacgc ctcggccgcc gcgacgagct cggccagcgg tcgcttctgc cactcgggca
     2281 cgagcgcgcg gtggtaccag gcggtggccg cgtaggtcgg caggaagagg gcatagggca
     2341 gctcgttgcc ctcgtcgaag ctgagggtct gcaggtcgac cgccatcgac accagcatca
     2401 cgccattcaa agagatgtcg tgcttgccct gcaggtgcag cgacagcccg gcggcgcgcg
     2461 tcgtgccgta gctctcgccg atcaggaact tggggctgcc ccagcggccg aagcgcgtca
     2521 ggtacatgcg gatgaattcg ccgaccgaat cgaggtcgcg ctggtactcg tggaattcgt
     2581 tcggcttctc gccctcggcc atgcgcgagt ggccggtgcc caccgggtcg atgaagacca
     2641 ggtcgctgtc ggtgagcagc gtgtgctcgt tgtcgaccag cgcgtagggc ggcggcggcg
     2701 cgtggccgaa ttcgtcgcat gcgacgcgtt gcggcccgag gatgcccaga tgcagccaca
     2761 cgctgctcga gcccgggccg ccgttgaagc tgaaggtgac ggggcgctgc tcgggcttct
     2821 tcgtcttcag cgtgtaggcg acgaagaaga agctcgcggt ggccttgtcg ggcttcttcg
     2881 ggttcgtgtc cttcgggtcg tagctgggga tgtagtcgcg cagcacgacg gtgccgcagg
     2941 tcgcggtgta ctcgagcgtc ttcttgccga cgaagagccg gtgctcggtc gtgaccaggc
     3001 gctctttcgg ttcggccggc gccggcgccg gcggggtggc ggtgctgtcc ttgctgttgt
     3061 ccggtgcgac catgggcagg tgctcgttag agtgcggaaa tgaagatgct tcgactctac
     3121 ctgcgccgat gaccggcgcg cctcgccgct tccccgccct cgcgggcgtg gatttcacga
     3181 gccggccgac ccggcgcaag ccgatcacgg tggcgctggg tcacgtccag catggcgcgc
     3241 tgcggctgga ccgcatcgaa tcgcacgccg acttcgacag cttcgcgcgc tggttgcaaa
     3301 cccccggccc gtggctcggc gtgttcgacc tgccgttcgg cctgccgcgc gaactcgtca
     3361 cgacgctgcg ctggccgctg cagtgggaag cgctgatgcg ccactacgcc gcgctgtcgc
     3421 gcgccgagat ccgtgccacg ttcgcggcgt tctgcgccgc gcgaccggcc ggcgccaagt
     3481 tcgcgcaccg cgccgccgac ggcccggccg gttcgtcgcc ggccatgaag tgggtcaacc
     3541 cgccggtcgc gttcatgctg cacgccggcg tgccgctgct gctggccgcc ggcgtgcacc
     3601 tgcccgggct gcacgacggc gacgcgatgc gcgtcgcgct cgagggctac cccgggttgc
     3661 tggcgcgcga agtgctcggc gcgcgctcgt acaagagcga caccaaggcc aggcagacca
     3721 gcgaacggct gatcgcgcgc atcgacctcg tcgacgcgct cgaccagggc cgcacgcggc
     3781 tggcgctgcg cctgaagctg acgcacgcgc agcgcgaggc gctgatcagc gatgccagcg
     3841 gcgaccggct cgacgcggtg ctgtgcctga tgcaggccgc gtgggcgagc ctgcggccga
     3901 accacggcct gccccccacg atcgacccgc tcgagggctg gatcgtcact gccggcaccg
     3961 agctgggcgc ctgatgccgc tctgctaagg tcggccgcca tgcaagcatt caacctgctg
     4021 ctgctcgccg gcgggctgct ggtcttcatc agcctgctgg ccggcgtgct gtcgacgcgc
     4081 ctggggctgc cgttcctgct ggtgttcctg gtcgcgggca tgctggtcgg cgtcgacggg
     4141 ccggtcggcg tgcgcttcga caacgcgcag ctctcggcct gggtcggcaa tgccgcgctc
     4201 gcgatgatcc tgctcgaggg cggcatcagc acgcgcatgc agaccttccg cgcgggcttc
     4261 aggccggcct tgctgctggc cacgctcggc gtcgcgctga ccgcggcgat ggtcggcgcg
     4321 gtggcgatga gcgcgatgca cctggactgg cgccacggcc tgctgctcgg cgcgatcgtc
     4381 ggctcgaccg atgccgccgc ggtgttctcg ctgctgcgcc agaccggcgt gcgcctgagc
     4441 gagcgcgtct cggcgacgct ggagctcgag tcgggcctga acgacccgat ggcggtgttc
     4501 ctcgtgctgg cgctgatcgc gacgctgaag accgacggcg gcgtcgccga cgtggcctgg
     4561 ctgttcgtgc gccaggccgg cttcggggcc gcgttcgggt tgctcggcgg cgcgctcgtc
     4621 gcatggctgc ttgcgcggct gccgctgaac gtcgagcatg gcggcgtgct ggcgctgatc
     4681 gtcgtgtcgg cgggcatggc ggtgttcgcg gcggcgggcc tggtcgaggg ctcgggcttt
     4741 ctcgcgatct acctgttcgg gctgcggctg cgtgcgcgcg ccgagccggc cgcgcatgcc
     4801 gcgtcgtcgg cgctcgacgg cttcgcgtgg gccgcgcagg ccacgatgtt cctgctgctg
     4861 ggcttgctgg tctcgccgca cgacatgctg ctgacgctcg cgccgacgct ggcggt
//
LOCUS       DATMJB010000303         4377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_737839, whole genome shotgun sequence.
ACCESSION   DATMJB010000303 DATMJB010000000
VERSION     DATMJB010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737839"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="VNU48_06635"
     CDS             complement(<1..1697)
                     /locus_tag="VNU48_06635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWI10988.1"
                     /translation="MEALSVRAGLFAAAVLLCLYLPHVALDMVRRARAKRHASPWGWL
                     LGGAVAWGTSLWAALLFTLLARHGGLDLSHDAALLLASWLISVGTAFGLFAATAVALP
                     GWRGWLRTSAGLAVGLSASFGLMLVSLQPVNPWAALWVQSRGLTATVLGASVLLAAHI
                     LGSEGLLALRWRRIARGIPMAAGLALTGLLWLSIGDEVSSGAPDAVPGLPSEHAALLA
                     AVATVLSLAVTRFMSRIERHLVDRADGLERSLRQANSDLQKIAYHDALTGLPNRLVFE
                     DKLVAAVGRADQAKARLAVLFIDLDGFKPINDSFGHSSGDAVLRQVGDRLRRLSRSND
                     TMARVGGDEFLMLLEGEADEQTAAQVATRMLATLSEPYSIGSREVAVSCSIGIVFYPD
                     GGTQTKLIARADAAMYAAKRSGGACYCFFEASMEADAHEQLDLQRDLRLALEKNELEL
                     YYQPKVDAHSGKVTAAEALLRWKHATKGMIGPSTFIPVAERFGLIGALGNWVIEDACR
                     QSRAWRDKGLRMRVAINLSAFQMRQDDLVERITTALARHRIDPSLLTCEITESVAME"
     gene            1935..2303
                     /locus_tag="VNU48_06640"
     CDS             1935..2303
                     /locus_tag="VNU48_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10989.1"
                     /translation="MLTWATCHWWYGRKRRRLEHRFGKLQADRDGLQEQVKRARQQVA
                     QLQKELSTWRLASVTTAPGKADGARAIEPPAVAPPTRRVVPELPAGLVFETPQLAAHG
                     FADTQPFDSDAVAGLARKRS"
     gene            2337..2753
                     /locus_tag="VNU48_06645"
     CDS             2337..2753
                     /locus_tag="VNU48_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10990.1"
                     /translation="MRKLLALLRWPRASARTPPGGRGPKPRELMTDSDWVRHRAPPRH
                     RDEQLSGDARAWLAELPEDFRPAALAERYPRIANRLAQLWRDPGLTEHLLDELVLPRR
                     PGRQGFPPEVMVDLQSLQVLNDHRLYLSEDPDPREP"
     gene            complement(2750..3700)
                     /locus_tag="VNU48_06650"
     CDS             complement(2750..3700)
                     /locus_tag="VNU48_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010110165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HWI10991.1"
                     /translation="MQNPALKVVVLGTGGTIAGSSTDASDNIGYRAAQRGVADLVRGV
                     PALAGVSLELEQVAQLDSKDMDHAVWRALALRCAFHLARPDVAGLVVTHGTDTLEETA
                     WFLQRVLAPAKPVVLAAAMRPATALQSDGPQNLLDAVGLARAAGARGVVAVLGGQVFG
                     ARDVRKVHPYRIDAFSAGDAGPVGVIEEGRLRLFRAWPQDGAVLDVTALPGDATAWPF
                     VAIVTSHAGASGAVVSALREAGVRGLVVAATGNGSVHRELEHALLQAQRDGVQVLRST
                     RCANGRVLDADAPALPSADDLTPAKARVELMLRLMTTQPS"
     gene            3804..>4377
                     /gene="lexA"
                     /locus_tag="VNU48_06655"
     CDS             3804..>4377
                     /gene="lexA"
                     /locus_tag="VNU48_06655"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWI10992.1"
                     /translation="MYEPPKLTDRQQQILDLVQSAIERTGAPPTRAEIASELGFKSAN
                     AAEEHLQALARKGVIELVGGTSRGIRLRSETLRQLNLQRGKQFSLPLPSLAQLTLPLI
                     GRVAAGSPILAQEHIDQTYLMEASMFPRRPDYLLKVRGMSMRDAGIIDGDLLAVQRAK
                     DAKNGQIVVARLGDEVTVKRFRRTRNSIELI"
BASE COUNT          702 a         1600 c         1495 g          580 t
ORIGIN      
        1 tcttccatcg cgaccgattc ggtgatctcg caggtcagca gcgacgggtc gatgcggtgc
       61 cgcgcgagtg cggtggtgat gcgctcgacc aggtcgtcct gacgcatctg gaacgccgac
      121 aggttgatcg ccacgcgcat gcgcaggcct ttgtcgcgcc aggcgcgcga ctgccggcag
      181 gcgtcttcga tgacccagtt gccgagcgcg ccgatcagac cgaagcgttc ggccaccggg
      241 atgaacgtcg acgggccgat catgcccttg gtcgcgtgct tccagcgcag cagcgcctcg
      301 gccgcggtga ccttgccgct gtgggcgtcg accttgggct ggtagtacag ctcgagctcg
      361 ttcttctcca gcgccaggcg caggtcgcgc tgcaggtcga gctgctcgtg cgcgtcggcc
      421 tccatgctgg cctcgaagaa gcagtagcag gcgccgccgg agcgcttggc tgcgtacatc
      481 gccgcatccg cgcgcgcgat cagcttggtc tgcgtgccgc cgtcggggta gaacacgatg
      541 ccgatcgagc acgacaccgc gacctcgcgg ctaccgatcg aatacggctc gctcaaggtg
      601 gccagcatgc gcgtggcgac ctgcgcggcg gtctgctcgt cggcctcgcc ttcgagcagc
      661 atcaggaact cgtcgccgcc gacgcgcgcc atcgtgtcgt tggagcgtga cagtcggcgc
      721 agccggtcgc cgacctggcg cagcacggcg tcgccgctgc tgtggccgaa cgagtcgttg
      781 atcggcttga agccgtcgag gtcgatgaac agcaccgcca ggcgcgcctt ggcctggtcc
      841 gcgcggccga ccgcggccac cagcttgtcc tcgaacacca gccggttcgg caacccggtg
      901 agcgcgtcgt ggtaggcgat cttctgcagg tccgaattgg cctggcgcag cgagcgctcg
      961 agaccgtcgg cgcgatcgac caggtggcgc tcgatgcgcg acatgaagcg cgtcacggcc
     1021 agcgacagga cggtggcgac ggcggcgagc agcgccgcgt gctcggacgg caggcccggc
     1081 accgcgtcgg gcgcaccgga cgagacctcg tcgccgatcg acagccacag caggccggtc
     1141 aatgccagcc cggcagccat cgggatgccc cgggcgatac gccgccagcg caacgcgagc
     1201 agaccttcgg agccgaggat gtgggcagcc agcagcacgc tggcgccgag cacggtcgcc
     1261 gtcagccccc gggactgcac ccacagcgcc gcccacgggt tcaccggttg caacgacacc
     1321 agcatcaggc cgaacgacgc gctcaggccc accgcgaggc cggcgctggt gcgcagccag
     1381 ccgcgccagc ccggcagcgc caccgcggtg gccgcgaaca gcccgaacgc cgtgccgacg
     1441 ctgatcaacc aggacgccag cagcagcgcg gcgtcgtgcg acaggtccag gccaccgtgg
     1501 cgcgcgagca gcgtgaacag caaggccgcc cacaggctcg tgccccacgc caccgcgccg
     1561 cccagcagcc agccccacgg gctcgcgtgg cgcttggcgc gcgcgcgtcg caccatgtcc
     1621 agcgccacat gtggcaggta caggcacagc agcacggctg ccgcgaacag cccggcgcga
     1681 acggacaagg cctccatggg ttccctttca gcaggatcgc cgcacggcga cgaaacacgc
     1741 cccaaacgac agcacgtgat ccgtgccccg accgaggtac ctctggctta tcggttaccg
     1801 acaaggcaaa cttgatgcga gacgggtgac cggcgccgaa catcgaggct caagaatccg
     1861 cgcggttaac cgataagctg ctgaccatgc aagaaatcac atcctcctgg ttgttccttg
     1921 caccactcgg tgtcgtgctg acctgggcca cgtgccactg gtggtacggg cgcaagcgcc
     1981 gccggctcga acaccgattc ggcaagttgc aggcggatcg ggacgggttg caggagcagg
     2041 tcaaacgcgc ccgccagcag gtcgcccagc tccagaagga gctgtcgacc tggcgcctgg
     2101 catcggtgac cacggcaccc ggcaaggccg acggtgcgcg tgcgatcgaa ccgccggccg
     2161 tggcgccgcc gacccgtcgc gtggtgcccg aacttccggc gggcctggtg ttcgaaacgc
     2221 cccagctggc ggcgcacggc ttcgccgaca cccaaccgtt cgacagcgac gccgtcgccg
     2281 gcctcgcccg caaacgttcg taggcgcgga ccccgcggcc gcgtcgcagc gccaccatgc
     2341 gcaagctgct cgcgctcctg cgctggcccc gggcatcggc acgcaccccg cccggcggac
     2401 gcggcccgaa gccgcgcgag ctgatgaccg attcggactg ggtccggcat cgcgcgccgc
     2461 cacgccatcg cgacgagcag ctgtcgggtg acgcgcgcgc ctggctcgcc gagctgcccg
     2521 aggatttccg gcccgcggcc ctggccgaac gctacccgcg catcgccaac cgcctggcgc
     2581 agctgtggcg cgacccgggc ctgacggagc acctgctcga cgagctggtg ctgccgcggc
     2641 gccccggccg acaaggtttt ccgcccgagg tgatggtcga cctgcagtcg ctgcaggtgc
     2701 tgaacgacca ccgcctgtac ctgtcggaag atccggaccc gcgggaaccc taactcggct
     2761 gcgtcgtcat cagccgcagc atcagctcga cccgcgcctt cgccggcgtc aggtcgtcgg
     2821 ccgagggcag cgccggcgca tcggcgtcca gcacgcgacc gttggcgcag cgcgtgctgc
     2881 gcagcacctg cacgccgtcg cgctgcgcct gcagcagcgc gtgctcgagt tcccggtgca
     2941 cgctgccgtt gccggtggca gccaccacga ggccccgaac gccggcctca cgcagggccg
     3001 acacgaccgc accgctcgcc ccggcgtggc tcgtgacgat cgccacgaac ggccacgccg
     3061 tggcgtcacc gggcagagcc gtgacgtcga gcacggcgcc gtcctgcggc caggcccgga
     3121 acaggcgcag gcggccctct tcgatcacgc cgaccggtcc ggcatcgccg gcgctgaacg
     3181 cgtcgatccg atagggatgg accttgcgca cgtcgcgcgc gccgaacacc tggccaccga
     3241 gcaccgcgac cacgccgcgt gcacccgcgg cacgtgcgag gccgaccgcg tcgagcaggt
     3301 tctgcggccc gtccgactgc agggcggtgg ccgggcgcat cgctgcggcc agcacgaccg
     3361 gcttggccgg cgcgagcacg cgttgcagaa accaggcggt ctcctcgagc gtgtcggtgc
     3421 cgtgcgtgac gacgagcccg gcgacgtcgg gacgtgccag atgaaacgca cagcgcagcg
     3481 cgagcgcgcg ccagaccgcg tgatccatgt ccttgctgtc gagctgcgcg acctgctcga
     3541 gctcgagcga gaccccggcc agcgccggca cgccgcgcac aaggtcggcg acgccgcgtt
     3601 gcgcggcacg gtagccgatg ttgtccgacg cgtccgtcga gctgcccgcg atcgtgccgc
     3661 cggtgccgag cacgacgacc ttgagtgctg ggttttgcat ttttctcgaa gctggatgaa
     3721 aatacaggtg ctggatgaat cgccagtgac cgcccgcgcc gggtgggcgc ggcgggcatt
     3781 cgcgccatca tcgaggatat tccgtgtacg aaccgcccaa gctcaccgac cggcaacagc
     3841 agattctcga tctggttcaa agcgcgatcg aacgcaccgg cgcgccgccc acgcgcgccg
     3901 agatcgccag cgaactcggc ttcaagtcgg ccaacgccgc cgaggagcac ctgcaggcgc
     3961 tggcgcgcaa gggcgtgatc gaactcgtgg gcggcacctc gcgcggcatc cggctgcgct
     4021 ccgagacgct gcgccagctg aacctgcagc gcggcaagca gttcagcctg ccgctgccca
     4081 gcctcgccca gctgaccctg ccgctgatcg gcagggtcgc ggccggcagc ccgatcctgg
     4141 cacaggagca catcgaccag acctacttga tggaggcgag catgttcccg cgccggcccg
     4201 actacctgct gaaggtgcgc ggcatgagca tgcgcgacgc cggcatcatc gacggcgacc
     4261 tgctcgcggt gcagcgcgcg aaagacgcga agaacggcca gatcgtcgtt gcacggctgg
     4321 gcgacgaggt cacggtcaag cgctttcgcc gcacccgcaa cagcatcgag ctgatcc
//
LOCUS       DATMJB010000304         2839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_254503, whole genome shotgun sequence.
ACCESSION   DATMJB010000304 DATMJB010000000
VERSION     DATMJB010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254503"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..909
                     /locus_tag="VNU48_06660"
     CDS             208..909
                     /locus_tag="VNU48_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI10993.1"
                     /translation="MFNDVSQFADSVTLASANAPRALPDRALRWFAATLDEVDYGMLL
                     LTNEVRVMHVNHAARIELDAAHPLQLLGRELRARRPQDVLPLADALHAAARRGLRKLL
                     ALGDGAHRISVSVVPLSRPDDADGPVTLLMLGKRRVCEVLSAQGFAKSHGLTAAEARV
                     LTALCRGTPPVEIAAEVGVAISTVRTQIGNIRVKTGATSIRALVQQVAALPPLMGVLR
                     EPTGAEHALRGLLGA"
     gene            989..1639
                     /locus_tag="VNU48_06665"
     CDS             989..1639
                     /locus_tag="VNU48_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWI10994.1"
                     /translation="MPPAASPSDPLAALPESLRLLARRGELRRYRKGHRLIEDGDRGD
                     TLFIIVSGRLRAFGSGDRDREITYGSYGPGEYLGEMSLDGGPRSANVEAIEPSQCVVV
                     TSQTLLRHIAEHPEFALELLAKVIRRARAATLSAKQLALNDVYGRLVLLLNELAEPQP
                     DGQRLILERPTHREMSNRLGCSREMVSRLLKDLEAGGYTAPRGAALVILRPLPARW"
     gene            complement(1653..2513)
                     /locus_tag="VNU48_06670"
     CDS             complement(1653..2513)
                     /locus_tag="VNU48_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWI10995.1"
                     /translation="MNEHEAQRRRSVCAGLLAGGALLAWPAARAREGVDVGPRSSFSQ
                     LVSAEQIEQAAQQQYLQLRQQAAAKGAIAGPGNPQLLRLRAIAQRLIPFTYEWNERAR
                     QWRWEINLIVSPQINAFCMPGGKIAFFTGILDKLGLSDDEVAMVMGHEMTHALREHAR
                     ERIGKTAATRGAIELGAAILGLGNGGRYLAGMGGQLLTLQFSRSDESEADLVGMELAA
                     RAGYDPHAGVTLWQKMAAANKSAPPQWISTHPSGPTRIRDIERSLPQVEPLYRRAPKP
                     PQRFDAPRGG"
     gene            complement(2564..>2839)
                     /locus_tag="VNU48_06675"
     CDS             complement(2564..>2839)
                     /locus_tag="VNU48_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI10996.1"
                     /translation="GGMGTAAFLAFLMSLCNQRFTATQFALLSAFASVGRVWVGPLAG
                     VLAESIGWPVFFVVSTVLALPALLMLWWMGASVRALEYDPAAPIADD"
BASE COUNT          389 a         1058 c         1006 g          386 t
ORIGIN      
        1 tgtgcccgca ggagatcgtc gacatgaacc gctggctggt tcgagtgctc gcgtgaacgt
       61 gcggggaggc gcgccggcgc cgcccgcggc gaggtaaata agtcacaggc cggcgcgggc
      121 atattcacca ctccgcgccg acccctcgac ctacgcttgt tccaacgccg gcccgccatg
      181 tcgcgagccg ggccaaggag gtaggtcatg ttcaacgacg tctctcagtt cgccgactcc
      241 gtcaccctcg cgagcgcgaa tgcgccccgc gcgctgccgg accgcgccct gcgctggttc
      301 gccgccacgc tcgacgaggt cgactacggc atgctgctgc tgaccaacga ggtgcgcgtg
      361 atgcacgtga accacgccgc gcgcatcgag ctcgatgcgg cgcacccgct gcagctgctg
      421 ggccgcgaac tgcgcgcgcg ccgcccgcag gacgtgctgc cgctcgccga cgcgctgcat
      481 gccgcggcgc gacgcggcct gcgcaagctg ctggcgcttg gcgacggcgc gcaccgcatc
      541 agcgtctcgg tcgtgccgct gtcgcggccg gacgacgccg acggcccggt gacgctgctg
      601 atgctgggca agcgccgggt ctgcgaggtg ctgtcggcgc agggcttcgc caagagtcac
      661 ggcctgaccg ccgccgaggc gcgcgtgctg accgcgctgt gccgcggcac gccgccggtc
      721 gagatcgcgg ctgaggtcgg cgtggcgatc tcgaccgtgc gcacgcagat cggcaacatc
      781 cgcgtgaaga ccggtgccac cagcatccgc gcgctggtgc agcaggtcgc ggcgctgccg
      841 ccgctgatgg gcgtgctgcg cgagcccacg ggcgcggagc acgcgctgcg cgggttgctc
      901 ggcgcctgaa cgcgcgccgc ggcggccggc gcggccggct agcatcgggc cctcacgccg
      961 gcaccgccac tcgggccgcg tggcccgcat gccaccagcc gcctcgcctt ccgatccgct
     1021 cgccgcgctg cccgaatccc tgcgcctgct ggcacgccgc ggcgagctca ggcgctaccg
     1081 caaggggcat cgcctgatcg aggacggcga ccgcggcgat accctgttca tcatcgtcag
     1141 cggccggctg cgcgccttcg gcagcggcga ccgcgaccgc gagatcacct atggcagcta
     1201 cggcccgggc gagtacctcg gcgagatgag cctggacggc gggccgcgtt cggccaacgt
     1261 cgaggccatc gagcccagcc agtgcgtggt cgtgacctcg cagacgctgc tgcgccacat
     1321 cgccgagcac ccggagttcg cgctcgaact gctcgccaag gtgatccgcc gtgcacgcgc
     1381 cgccacgctg agcgcgaaac agctggcgct gaacgacgtc tacggccggt tggtgctgct
     1441 gctgaacgag ctggccgagc cgcagcccga cggccagcgg ctgatcctcg aacgcccgac
     1501 acaccgcgag atgtcgaacc gcctgggttg ctcgcgcgag atggtcagcc ggctgctcaa
     1561 ggacctcgaa gccggcggct acaccgcgcc gcgcggcgcg gcgctcgtga tcctgcggcc
     1621 gctgccggcg cgctggtaga gccgcgcccg cgtcagccgc cgcgcggcgc gtcgaagcgc
     1681 tgcggcggtt tcggcgcgcg ccggtacagc ggctcgacct gcggcaggct gcgctcgatg
     1741 tcgcggatgc gcgtcggccc cgacgggtgc gtcgagatcc actgcggcgg cgcgctcttg
     1801 ttcgcggccg ccatcttctg ccacagggtc acgccggcgt gcgggtcgta gccggcgcgc
     1861 gcggcgagtt ccatgccgac gaggtcggcc tcggattcgt cggagcggct gaactgcagg
     1921 gtcagcagct gcccgcccat gccggcgagg tagcgcccgc cgttgcccag ccccaggatg
     1981 gccgcaccga gctcgatcgc gccgcgcgtc gccgcggtct tgccgatgcg ctcgcgcgcg
     2041 tgctcgcgca gcgcgtgggt catctcgtgg cccatcacca tcgcgacctc gtcgtcgctg
     2101 agcccgagct tgtcgaggat gccggtgaag aacgcgatct tgccgcccgg catgcagaac
     2161 gcattgatct gcgggctgac gatcaggttg atctcccagc gccactgccg cgcgcgttcg
     2221 ttccactcgt aggtgaacgg gatcaggcgc tgcgcgatcg cgcgcaggcg cagcagctgc
     2281 gggttgccgg gcccggcgat cgcacccttc gcggcggcct gctgccgcag ttgcaggtac
     2341 tgctgctgcg cggcctgctc gatctgctcg gccgagacca gctggctgaa gctcgaacgc
     2401 gggccgacat cgacgccctc gcgcgcccgc gccgccggcc acgccagcag cgcgcccccc
     2461 gcgagcaggc cggcgcagac cgaacggcgc cgctgcgcct cgtgctcgtt catcgatttt
     2521 tcctccgcat gccggacatc agttcggggt cgggcgcccg gattcaatcg tcggcgatcg
     2581 gcgcggccgg atcgtattcg agcgcgcgca ccgacgcgcc catccaccac agcatcagca
     2641 gcgccggcag cgcgagcacg gtcgagacga cgaagaacac cggccagccg atcgactcgg
     2701 ccagcacgcc ggcgagcggc ccgacccaga cgcgcccgac cgacgcgaac gcgctgagca
     2761 gcgcgaactg ggtcgcagtg aagcgctggt tgcacaggct catcaggaac gcgagaaagg
     2821 ccgcggtgcc catgccgcc
//
LOCUS       DATMJB010000305         3174 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_180674, whole genome shotgun sequence.
ACCESSION   DATMJB010000305 DATMJB010000000
VERSION     DATMJB010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180674"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VNU48_06680"
     CDS             <1..314
                     /locus_tag="VNU48_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10997.1"
                     /translation="ASLLRDKEASKSDYIRSRTVARLLFEKLPALEAIVYQGVALEGS
                     FNMALKPDVADRVLYNGATFVLRVIKRYDHGFYDFEAVNRAKGYFGDGTIKWGAPTTP
                     V"
     gene            complement(494..2761)
                     /locus_tag="VNU48_06685"
     CDS             complement(494..2761)
                     /locus_tag="VNU48_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10998.1"
                     /translation="MTRCAAARVASAALVLLAMFGAGASAQTPPDPGAPSWRFEPARD
                     AFSADALLDLRDLNERVAGETGFVRVGADGEFLRGDGAPLRFWAVNTDVGRGPFVATP
                     LGPKTAPDLARHARFLAKRGVNMVRLHRQLSPDLRAHPDAAIGDINEVERDGIWRTVA
                     AMRKEGIYTTISPYWAANMKFSKAWGLPGGAEQPAWGLLFFDETLQAAYRVWLKKLLT
                     EKNPYTGIALAQDPSVAIIQLQNEDSLLFWTVGTIKGAQRQALEALYARFLMRKHGSL
                     ANAVAAWKGDHANGDAPESGRLALLELWELTQPGAGGHAARLADQTEFYARTMHEFNR
                     GTVDYLKRELGVRQLVNAGNWKTASSARLNDAERWSYTAADVDAVNVYTGGVHQGPAS
                     GWAVLNGDKFTSESVLRQPGALPINLRRTLGRPMLVTEGGWIAPNGFGAEGPFLIAAY
                     ASLTGIGGYYWFSTTDEGWAPPQSANGHLASLQKWSFAGPDVLGTFPAAALAYRKAYL
                     RRGAPALTETRSLQELWERKPPALVEASTFDPNRDVTRAAPGPGIGAVPGAAFLVGPA
                     RVAFGADPARSSVISFDKLIEPGLIRSNTGQILMSTAQAFCTVDAPMAQGVAAHFAQA
                     PVHQLGDVRFASGNAFGAALAVSLDGLPLSRSKRVLVQYGTQSRPTGWLQAPTEIAVK
                     GQAPGAGIEITSIGRAPWLVEAARLDVTIRNPGLTSATVLDMNGMPAGTVPLGRSATQ
                     VDFRFPAAAMYVLLR"
     gene            complement(2758..>3174)
                     /locus_tag="VNU48_06690"
     CDS             complement(2758..>3174)
                     /locus_tag="VNU48_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI10999.1"
                     /translation="AGRHTRTGRRRPARPDGAGVGIRIATLARVDSRYSSRPPPVTPD
                     ALRIEQALQQSAPLARLQSLMRDSKARFEAIRPALPAALAPHIKPGPVDETGWSLLAA
                     NASVAAKLRQLQPRLEELLRERGWELSAIRVKVQST"
BASE COUNT          469 a         1082 c         1126 g          497 t
ORIGIN      
        1 tggcctcgct gctgcgtgac aaggaggcct cgaagagcga ctacatacgt tcgcgaacgg
       61 tagcgaggct actttttgag aagctgccgg cgcttgaagc aatcgtctat caaggcgttg
      121 ctctcgaagg atcgttcaac atggcgttga aacccgacgt agcagatcga gtgttgtaca
      181 acggtgcgac gttcgtcctg agggtgatca aaaggtacga ccacgggttc tacgacttcg
      241 aggccgtcaa tcgtgccaag ggctactttg gggacggcac gatcaagtgg ggtgctccga
      301 cgacgccagt ctgacaggtg agttgtactc ggctgctttt cgagggcgat acgcacgcat
      361 gcgtgaggcg tctgacggta cttatgacgg tacgttgggt atggtcatcc gtagatgctt
      421 atataaatca acgccttacg tatcacattc gattccgata gccgcaccaa gacctcgcct
      481 cgcggcgacc cgctcagcgc agcagcacgt acatcgccgc cgccgggaag cggaagtcca
      541 cctgcgtggc cgagcgcccg aggggcaccg tgccggcggg catgccgttc atgtccagca
      601 ccgtcgccga ggtcaagccg gggttgcgga tcgtgacgtc gagcctcgcc gcttccacca
      661 gccacggcgc gcggccgatc gaggtgatct cgatgccggc gcccggcgcc tggcccttca
      721 ccgcgatctc ggtcggcgcc tgcaaccagc cggtcggccg gctctgcgtg ccgtactgca
      781 ccagcacccg cttcgaacga ctcagcggca ggccgtcgag cgagaccgcc agcgccgccc
      841 cgaacgcatt gcccgacgcg aagcgcacgt cgccgagctg gtgcaccggt gcctgggcga
      901 agtgcgcggc cacgccttgc gccatcggcg cgtcgaccgt gcagaaggcc tgggccgtgc
      961 tcatgaggat ctgcccggtg ttcgatcgaa tcagcccggg ctcgatcagc ttgtcgaacg
     1021 agatgaccga ggaccgtgcc ggatcggccc cgaacgcgac ccgcgccgga ccgacgagaa
     1081 acgccgcacc cgggaccgcg ccgatgcccg ggcccggggc cgcacgtgtg acgtcgcggt
     1141 tcgggtcgaa ggtgcttgcc tcgaccagcg ccggcggctt gcgctcccac agctcctgca
     1201 gcgaccgggt ctcggtcagc gccggcgcgc cgcgccgcag gtaggccttg cggtaggcga
     1261 gtgcggcggc cgggaaggtg ccgagcacat cggggccggc gaacgaccat ttctgcagcg
     1321 acgccagatg accgttcgcg gactgcggcg gcgcccagcc ttcgtccgtc gtgctgaacc
     1381 agtagtagcc gccgatgccg gtcagcgacg cgtaggccgc gatcaggaac gggccctcgg
     1441 cgccgaagcc gttcggcgcg atccagccgc cttcggtgac gagcatcggc cggccgagcg
     1501 tgcgcctcag gttgatcggc agcgcgcccg gctggcgtag cacgctttcg ctggtgaact
     1561 tgtcgccgtt cagcaccgcc cagccgctgg ccgggccctg atgcacgccg ccggtgtaga
     1621 cgttgaccgc atcgacatcg gcagcggtgt acgaccagcg ctcggcatcg ttcaggcggg
     1681 cgctgctggc ggtcttccag ttgccggcgt tgacgagctg gcgcacgccg agttcgcgct
     1741 tcaggtagtc gaccgtgccg cgattgaact cgtgcatcgt gcgggcgtag aactcggtct
     1801 ggtcggccag gcgcgccgcg tggccgccgg cgccgggctg cgtcagctcc cagagttcga
     1861 gcagcgccag gcggccggac tcgggcgcgt cgccgttcgc gtggtcgccc ttccaggccg
     1921 ccaccgcgtt cgcgagcgag ccgtgcttgc gcatcagaaa gcgcgcatac agcgcctcga
     1981 gcgcctggcg ctgcgcgccc ttgatcgtgc cgacggtcca gaacagcagg ctgtcttcgt
     2041 tctgcagctg gatgatcgcc acgctcgggt cctgcgcgag cgcgatgccg gtgtacggat
     2101 tcttctcggt cagcagcttc ttcagccaca cgcggtacgc ggcctgcagt gtctcgtcga
     2161 agaacagcag gccccaggcg ggctgctcgg cgccgccggg caggccccag gctttcgaga
     2221 acttcatgtt ggccgcccag tacggcgaga tcgtcgtgta gatgccctcc ttgcgcatcg
     2281 cggcgacggt gcgccagatg ccgtcgcgct cgacctcgtt gatgtcgccg atcgcggcgt
     2341 cggggtgggc gcgcaggtcg gggctgagtt ggcggtgcag ccgcaccatg ttgacgccgc
     2401 gcttggccag gaagcgcgcg tgccgcgcga gatcgggcgc ggtcttcggg cccagcggcg
     2461 tggccacgaa cgggccgcgg ccgacatcgg tgttgacggc ccagaagcgc agcggcgcgc
     2521 cgtcgccgcg caggaattcg ccgtcggcgc cgacgcgcac gaagccggtc tcgccggcga
     2581 cccgctcgtt caggtcgcgc aggtcgagca gcgcgtcggc actgaaggca tcgcgcgccg
     2641 gctcgaagcg ccagctcggc gcgccggggt cgggcggggt ctgcgccgac gcgccggcgc
     2701 cgaacatcgc gagcagcacc agcgccgcgc tcgcgacgcg ggcggcggcg catcgcgtca
     2761 tgtcgattga accttcaccc ggatggcgct gagctcccag ccccgctcgc gcagcagctc
     2821 ttcgaggcgt ggctgaagct ggcgcagctt ggcggcgacc gatgcattgg cggcaagcag
     2881 cgaccagccg gtctcgtcga ccgggccggg cttgatgtgc ggtgccagcg ccgccggcag
     2941 agcggggcga atcgcctcga agcgcgcctt cgagtcgcgc atcagcgatt gcaaccgcgc
     3001 cagcggcgcg ctttgctgca gggcctgttc gatgcgcaac gcatcggggg tgaccggcgg
     3061 cggtcgcgat gagtacctgg aatccacccg ggcgagtgta gcgatgcgga ttccgacccc
     3121 ggcgccgtcc gggcgcgccg gccgccgccg accggtgcgc gtgtgccgcc cggc
//
LOCUS       DATMJB010000306         6088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_426250, whole genome shotgun sequence.
ACCESSION   DATMJB010000306 DATMJB010000000
VERSION     DATMJB010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426250"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..742
                     /gene="pstB"
                     /locus_tag="VNU48_06695"
     CDS             5..742
                     /gene="pstB"
                     /locus_tag="VNU48_06695"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWI11000.1"
                     /translation="MRNLNFYYSTFHALKNINLDIPENKVTAFIGPSGCGKSTLLRTF
                     NRIYSLYPEQRADGEILLDGQNVLSSSIDVNELRARVGMVFQKPTPFPMSIYDNIAFG
                     VKLFETLPRIEMDERVEWALKKAALWNEVKDKLNQSGSGLSGGQQQRLCIARGIAIKP
                     EVLLLDEPCSALDPISTGKIEELIHELKSDYTVVIVTHNMQQAARSSDYTAYMYLGDL
                     IEFGPTSDVFMKPKKKDTEDYITGRFG"
     gene            756..1463
                     /gene="phoU"
                     /locus_tag="VNU48_06700"
     CDS             756..1463
                     /gene="phoU"
                     /locus_tag="VNU48_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWI11001.1"
                     /translation="MSDKHLSTQFDAELSGISTRVLEMGGMVESQVARSVYALTNFSA
                     EIASDVLATEERVNQTEIEIDRDLSTIIARRQPTARDLRLLIAISKTIANLERVGDEA
                     ARIARTVQRLLNSGVSSRLRLPVKDLAYEAELAVSQLRKALDAFARLDVDKAVEVLKQ
                     DDLIDQEFEGLMRKLITFMMEDPRTISSSIDLVFVAKAIERVGDHAKNLAEQIIYIVK
                     GTDVRHNSVGDVESLVR"
     gene            1477..2217
                     /gene="phoB"
                     /locus_tag="VNU48_06705"
     CDS             1477..2217
                     /gene="phoB"
                     /locus_tag="VNU48_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HWI11002.1"
                     /translation="MSHILVVEDETAIAELISINLRHAGFEVTIAATAEQAQAAVDGV
                     LPDLIILDWMLPGQSGLALAKRWRGEARTREMPVIMLTARADEADKIQGLDAGADDYL
                     TKPFSTNELMARIRAVLRRKAPEALDTVVEVGPLKIDPATRRVSRGLGADMRDVKVGA
                     TEFRLLHFFMTHPERVHSRAQLLDRVWGDHVFIEERTVDVHVKRLREALSPVGCAQMI
                     ETVRGAGYRLTQQGATTASSVPAPALSA"
     gene            2243..3598
                     /gene="phoR"
                     /locus_tag="VNU48_06710"
     CDS             2243..3598
                     /gene="phoR"
                     /locus_tag="VNU48_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HWI11003.1"
                     /translation="MSWLLQRLVAVLIATALGGAAGLSIPGHSVPLFGALLGFAFALG
                     VIGVRDTLNGYRLIAWLRGSQEEPAPRDAGLWGELGYRVERSIRLRERAAVLERDRLT
                     QFLSAIEASPNGVMLLDARDQIEWCNSLAADHFGLDPQRDRRQPVTNLVRAPAFVSYL
                     QAGRFDEAVLVPDPRGRRTLSVLVRSYGDGLRLVLSQDITEHERTDGMRRDFVANVSH
                     EIRTPLTVLAGFIETMTNLPLTEVERKRVLNLMTQQADRMGTLVSDLLTLAQLEGSPR
                     PHADRWVALAPLFAQVAVDAKVLSAGRHTLVFPASTDAQIAGAPNELLSAITNLVNNA
                     VRYTPEGGRIELGWKVLTDGAGEVSVADTGPGIAREHLPRLTERFYRVDGSRSRDTGG
                     TGLGLSIVKHVAQRHGGELDIHSELGRGSLFRLVFPAARVRAVAPVVPLALAREERVL
                     P"
     gene            complement(3608..5284)
                     /locus_tag="VNU48_06715"
     CDS             complement(3608..5284)
                     /locus_tag="VNU48_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11004.1"
                     /translation="MNAPNPALVSQRAAQPLPRLALLLFCAAYVIPGVFGRDPWKSAD
                     ITAFGYMVNIAQGRTSWLAPTVAGLPADAALLPYWLGASFIKLLGPWLDPALAARIPF
                     ALLLVLVLVLTWYSAYHLARTEAAQPLPFAFGGEADPIDYARAIADGALLALVASLGL
                     LQLGHETTPELAQLASVALLIYALAASPFRGPQPKLAVLAALPALAASGAPAIALALG
                     VVGGFICRASSYEPARRFVPWIAASALLSAALASALGAWGWRVGTSALSWPGAWELLR
                     LLAWFTWPAWPLAVWTVWRWRGHVFDRHIAIPLGAALVSVLACVAMGGSERALMLGLP
                     PLALLAAFALPTLQRSTAAAIDWFSVCFFTIAAAVIWVIYSSMQTGVPAQPAANIARL
                     SPGYTHGFSPFTLVLAVLATLAWLWLVKWRTGRSRHPLWKSLVLPASGVALCWLLVMT
                     LLLPPLDNARSYRSLIQRIAPRVPVGACIAAPGMPRAQLVALEYLGGYRVDALTSAAA
                     TRCEFLLQLETRTAPATPGPAWRLLARERRNTSEDEVTAIFRRRAGPTAR"
     gene            complement(5411..5671)
                     /locus_tag="VNU48_06720"
     CDS             complement(5411..5671)
                     /locus_tag="VNU48_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HWI11005.1"
                     /translation="MKEGIHPTYREVCFVDLSNGFKFVTRSCATAKETIKMEDGRELP
                     LFKLESTSESHPFYTGTQKSVDSLGGRVEKFRNKFAHLGAKK"
     gene            complement(5794..>6088)
                     /gene="rho"
                     /locus_tag="VNU48_06725"
     CDS             complement(5794..>6088)
                     /gene="rho"
                     /locus_tag="VNU48_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429510.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWI11006.1"
                     /translation="GSRMDEVIYEEFKGTGNCEIHLDRRMAEKRVYPSILINKSGTRR
                     EELLLKPEILQKTWILRKLLYAMDEIEAMEFVLDKMKSAKNNLDFFDMMRRGG"
BASE COUNT         1013 a         2057 c         2131 g          887 t
ORIGIN      
        1 ctcggtgcgc aacctgaact tctactacag caccttccac gcgctgaaga acatcaacct
       61 cgacatcccc gagaacaagg tcacggcctt catcggtccc tcgggttgcg gcaagtcgac
      121 gctgctgcgc accttcaacc gcatttacag cctgtatccc gagcagcgcg ctgacggcga
      181 gatcctgctc gacgggcaga acgtgctgtc gtcctccatc gacgtgaacg agcttcgcgc
      241 ccgtgtcggc atggtgttcc agaagccgac gccgttcccg atgtcgatct acgacaacat
      301 cgcgttcggc gtgaagctgt tcgagacgct gccgcgcatc gagatggacg agcgcgtcga
      361 gtgggcgctg aagaaggcgg cgctgtggaa cgaggtcaag gacaagctga accagagcgg
      421 ctcgggcctg tcgggcggac agcagcagcg gctgtgcatc gcgcgcggca tcgcgatcaa
      481 gcccgaggtg ctactgctcg acgagccctg ctcggcgctc gacccgatct cgaccggcaa
      541 gatcgaggag ctgatccacg aactgaagag cgactacacc gtcgtgatcg tcacccacaa
      601 catgcagcag gccgcgcgca gctccgacta cacggcgtac atgtacctcg gcgacctgat
      661 cgagttcggg ccgacgtccg acgtgttcat gaagccgaag aagaaggaca ccgaggacta
      721 catcacgggc cgcttcggct gacccaggag ccaccatgag cgacaaacac ctttccacgc
      781 agttcgatgc cgaactcagc ggcatctcga cccgggtgct cgagatgggc ggcatggtcg
      841 agtcgcaggt cgcacgctcg gtctacgcgc tgaccaactt cagtgccgag atcgcctccg
      901 acgtgctggc caccgaagag cgcgtgaacc agaccgagat cgagatcgac cgcgacctgt
      961 cgaccatcat cgcgcggcgc cagccgaccg cgcgcgacct gcgcctgctg atcgcgatct
     1021 cgaagacgat cgccaacctc gagcgcgtcg gcgacgaggc ggcgcgcatc gcgcgcaccg
     1081 tgcagcggct gctgaactcc ggcgtgtcga gccggctgcg cctgccggtc aaggacctgg
     1141 cgtacgaggc ggagctggcg gtctcgcagc tgcgcaaggc gctcgacgcg ttcgcacgcc
     1201 tggacgtcga caaggcggtc gaggtgctga agcaggacga cctgatcgac caggagttcg
     1261 agggcctgat gcgcaagctg atcacgttca tgatggaaga cccgcgtacg atctcgtcga
     1321 gcatcgacct ggtgttcgtc gcgaaggcga tcgagcgcgt cggcgaccac gcgaagaacc
     1381 tcgccgaaca gatcatctac atcgtcaagg gcaccgacgt gcgccacaac tcggtcggcg
     1441 acgtcgaatc gctggtgcgc tgatgaggag ttcgcgatga gtcacatcct ggtcgtcgaa
     1501 gacgagacgg cgatcgccga gctgatctcg atcaacctgc gccacgccgg tttcgaggtc
     1561 acgatcgcgg cgaccgccga gcaggcgcag gcggcggtcg acggtgtgct gccggacctg
     1621 atcatcctgg actggatgct gcccggccag tccggcctgg cgctggccaa gcgctggcgc
     1681 ggcgaggcgc gcacccgcga gatgccggtc atcatgctga cggcgcgcgc cgacgaggcc
     1741 gacaagatcc agggcctgga cgccggtgcc gacgactacc tgaccaagcc gttctcgacc
     1801 aacgagctga tggcgcgcat ccgcgcggtg ctgcggcgca aggcgcccga ggcactcgat
     1861 accgtggtcg aagtcgggcc gctgaagatc gacccggcga cgcgccgcgt cagccgtggg
     1921 ctgggcgcgg acatgcgcga cgtcaaggtc ggcgcgaccg agttccggct gctgcacttc
     1981 ttcatgacgc accccgagcg cgtgcacagc cgcgcgcagc tgctcgatcg cgtctggggc
     2041 gaccacgtgt tcatcgagga gcgcacggtc gacgtccacg tcaagcgtct gcgcgaggcc
     2101 ctgtcgccgg tcggctgcgc gcagatgatc gagacggtgc gcggcgccgg ctaccggctg
     2161 acgcagcagg gcgcgaccac cgcctccagc gtgccggcgc cggcactgtc ggcgtgaccc
     2221 gacgggcgcg gcccgtgcgg cgatgagctg gctgctgcag cgcctggtgg cggtgctgat
     2281 cgcgaccgcg ctcggcggcg ccgcgggcct gtcgatcccg ggccacagcg tgccgctgtt
     2341 cggtgcgctg ctcggcttcg cgttcgcgct cggcgtgatc ggcgtgcgcg acacgctgaa
     2401 cggctaccgc ctgatcgcct ggctgcgcgg ttcgcaggag gagccggcgc cgcgcgatgc
     2461 cggcctgtgg ggcgaactcg gctaccgcgt cgagcgctcg atccggttgc gtgagcgtgc
     2521 cgccgtgctc gagcgggacc ggttgacgca gttcctgtcg gcgatcgagg cctcgccgaa
     2581 cggcgtgatg ctgctcgatg cgcgtgacca gatcgagtgg tgcaactcgc tcgccgccga
     2641 ccacttcggc ctcgacccgc agcgcgaccg gcgccaaccc gtgacgaacc tggtgcgggc
     2701 gccggccttc gtgagctatc tgcaggcggg ccggttcgac gaggcggtgc tggtccccga
     2761 cccgcgcggc cggcgcacgc tgtcggtgct ggtgcgcagc tacggcgacg gtctgcgcct
     2821 ggtgctgtcg caggacatca ccgaacacga gcgcaccgac ggcatgcggc gcgatttcgt
     2881 cgccaatgtt tcgcacgaga ttcgcacgcc gctgacggtg ctggccggct tcatcgagac
     2941 gatgacgaac ctgccgctga cggaggtcga gcgcaaacgc gtactgaact tgatgacgca
     3001 gcaggccgat cgcatgggca cgctggtgag cgacctgctg acgctcgcgc agctcgaagg
     3061 cagcccgcgc ccgcatgccg atcgctgggt tgcgctcgcg ccgctgttcg cgcaggtcgc
     3121 ggtcgacgcc aaggtcctgt cggccgggcg tcacacgctg gtgttcccgg cgtcgaccga
     3181 cgcgcagatc gccggcgcac cgaacgagct gctcagcgcg atcaccaatc tcgtcaacaa
     3241 cgcggtgcgc tacacgcccg aaggcgggcg catcgagctg ggctggaagg tgctgaccga
     3301 cggtgccggc gaggtcagcg tggccgacac cgggcccggc atcgcgcgcg agcacctgcc
     3361 gcgcctgacc gagcgcttct accgtgtcga cggcagccgc tcgcgcgaca ccggcggcac
     3421 cggtctgggg ctgtcgatcg tcaagcatgt ggcgcagcgc cacggcggcg agctcgacat
     3481 ccacagcgag ctggggcgcg gctccttgtt ccggctcgtg ttcccggcgg cgcgcgtgcg
     3541 cgcggttgcg ccggtggtgc cgctcgcgct ggcgcgcgag gagcgcgtgc tcccctgaga
     3601 aggcggacta tcgagcagtc gggccggcgc gacggcggaa gatcgcggtg acctcgtctt
     3661 cgctggtgtt gcgtcgctcg cgcgcgagca gccgccaggc cgggccgggt gtggcgggtg
     3721 ctgtgcgcgt ctccaactgc agcaagaact cgcagcgcgt ggccgcggcc gatgtgagcg
     3781 cgtccacgcg atagccgccc aggtattcca gcgcgacgag ctgcgcgcgc ggcatgccgg
     3841 gcgcggcgat gcaggcgccg accggcacgc gcggcgcgat gcgctggatc agcgagcggt
     3901 agctgcgcgc gttgtcgagc ggcggcagca gcagggtcat caccagcagc cagcacagtg
     3961 cgacgccgct ggccggcagc accagactct tccacagcgg atgccggctg cgcccggtgc
     4021 gccacttgac gagccacagc cacgccaagg tggccagcac cgccagcacc agcgtgaacg
     4081 gcgagaagcc gtgcgtgtaa cccggcgaca ggcgcgcgat gttggcggcc ggttgcgccg
     4141 gcacgccggt ctgcatcgag ctgtagatga cccagatcac ggccgcggcg atcgtgaaga
     4201 agcagaccga gaaccaatcg atcgccgcag cggtgctgcg ctgcagggtc ggcagcgcga
     4261 acgccgccag cagcgccagc ggcggcaggc cgagcatcag cgcgcgttcc gagccgccca
     4321 tcgcgacgca ggcgagcacg ctgacgaggg ccgcgccgag cgggatcgcg atgtggcggt
     4381 cgaagacgtg gccgcgccag cgccagaccg tccagaccgc gagcggccag gctggccacg
     4441 tgaaccaggc gagcagccgc agcagctccc aggcgccggg ccaggacagc gcgctcgtgc
     4501 cgacgcgcca gccccaggca ccgagcgccg acgccagcgc ggccgacagc agcgcgctgg
     4561 cggcgatcca cggcacgaag cgacgcgccg gctcgtagct cgacgcgcgg cagatgaaac
     4621 cgccgacgac gccgagcgcg agcgcaatcg ccggcgcgcc gctggccgcg agcgcgggca
     4681 gcgcggccag caccgccagc ttgggctgcg gcccgcgaaa cgggctggcc gcgagcgcgt
     4741 agatgagcag cgcgacactc gccagctgcg caagctcggg cgtggtttca tggccgagct
     4801 gcagcagtcc gagcgacgcg accagcgcca gcagtgcgcc gtcggcgatc gcgcgggcat
     4861 agtcgatcgg gtccgcctcg ccgccgaacg cgaacggcag cggctgcgcg gcctcggtgc
     4921 gcgcgaggtg gtaggcgctg taccaggtca gcaccagcac cagcaccagc agcagcgcga
     4981 aagggatacg cgccgcgagg gccggatcga gccaggggcc gagcagcttg atgaacgacg
     5041 cgccgagcca gtacggcagc agcgcggcat cggccggcag gccggcgacg gtcggcgcga
     5101 gccacgaggt gcgcccctgc gcgatgttca ccatgtagcc gaacgcggtg atgtcggcgc
     5161 tcttccacgg gtcgcgaccg aacacgccgg ggatcacgta ggccgcgcag aacagcagca
     5221 gcgccagacg cggcagcggc tgcgcggcgc gctggctgac gagggcggga ttgggtgcgt
     5281 tcaattcggt gcgagcgggc tgaggcccga atgatgcagc aacgcgggca tcaaaaaagg
     5341 cagccgaagc tgcctatttc cgagccgtcc aaacggcggc cgagaccgcg tttgctcagc
     5401 agcggcgcga ctacttcttc gcgcccaggt gcgcaaactt gttgcggaac ttctcgaccc
     5461 ggccgccgag cgagtcgaca ctcttttgcg tgccggtgta gaacgggtgc gattcgctgg
     5521 tcgattcgag cttgaacagc ggcagttcgc ggccgtcttc catcttgatg gtttccttgg
     5581 ccgtcgcgca cgaacgcgtc acgaacttga aaccattgga caggtccacg aagcagactt
     5641 cgcggtacgt ggggtgaatg ccttctttca tgaggagcct cgctgatgtt gccccggcga
     5701 ccaggtcgcc gtcgttacag aagccacgcc gaaccgttcg gcgcactttc cgctatgcgg
     5761 gagaaaccgc gaattatagc ctgatgggtg cagctaaccg cctcgccgca tcatgtcgaa
     5821 gaagtcgagg ttgttcttcg ccgacttcat cttgtcgagc acgaactcca tcgcctcgat
     5881 ctcgtccatc gcgtagagca gcttgcgcag gatccaggtc ttctgcagga tttccggctt
     5941 cagcagcaac tcttcgcgcc gcgtgccgct cttgttgatc aggatcgacg ggtagacgcg
     6001 cttttcggcc atccggcggt cgaggtggat ttcgcagttg ccggtgccct tgaactcttc
     6061 gtagatcact tcgtccatgc ggctgccg
//
LOCUS       DATMJB010000307         4816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_703346, whole genome shotgun sequence.
ACCESSION   DATMJB010000307 DATMJB010000000
VERSION     DATMJB010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703346"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VNU48_06730"
     CDS             complement(<1..1160)
                     /locus_tag="VNU48_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWI11007.1"
                     /translation="MKTLLRQAAARVVCVLALACVASNAAPPPRGAERGPPQRAVAVA
                     AETEARVIVKYKADAALTRALPGEAAGHGAPPQHAQALAARLGLALSDGRTIGAHAQV
                     LKGRGMSSQDLADRLAAQDDVEYAVVDGRMHALAAPSDPLYPNNLAAPTTPVTGQWYL
                     RAPTSATIVDATSVVSAINAQAAWDVTTGNPAIVVAMLDTGVRFDHPDLSSKLLAGSD
                     FISNTLTANDGTARDSDASDPGDYVTSADVGAAVPGCTSSDVGNSSWHGTQTAGLVGA
                     ATNNGVGMAGTGRDIMLLPLRVLGKCGGFDSDIQAAMLWAASLESGSTTTVPRARVIN
                     MSLGASGACSAAYQDAIQQVNARGVVVVVAAGNEGLAVGTPANCPGAIAVAGV"
     gene            complement(1319..2653)
                     /locus_tag="VNU48_06735"
     CDS             complement(1319..2653)
                     /locus_tag="VNU48_06735"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HWI11008.1"
                     /translation="MKNFSWWSVVGLTAATVAVSGCGSGGGDSSSANVRVANATLSPS
                     HASIDLLVNAAIVPPAVAVGTVSAYAAPAAGSVTLQVNDAGGATALVTAVPTLTGGNH
                     YTVVAYESGGVVKFTPAIGEDVALPAAGVTTLRIYDAAIEAGAVDVYITAGACTTANL
                     TTISPAANFGAIVSVAATTVTQGAGTYNVCATGSGSKTDIRMSLPVTLAGQTVATVLM
                     TPTSGGALINGALLVQQGAYTAVPNPNARVRLAAAVSGGATVTALASDSTLSAPITID
                     SSVAPAFGFYALVPATSSLNVNVGVGNSVQVPGSLVAGSDATLLVYGSPSSSTATLIA
                     DDNRPPTSATATRLRLINGVTGSAGTLTLTANSAPVGINIGAGQWSSYQSLIGTTNNA
                     GTANTFSFGLTSSTAGPLTGLLGASGTGTLNSNATYSVLAVGDISAPQLLTR"
     gene            2924..3646
                     /locus_tag="VNU48_06740"
     CDS             2924..3646
                     /locus_tag="VNU48_06740"
                     /inference="COORDINATES: protein motif:HMM:NF008437.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HWI11009.1"
                     /translation="MRRAIRPVLAAVAAAGAALTAPTAFAADYATVVSSTPVAASVPV
                     RRQVCNDTQQYVQQAPSGAGAVVGAIAGGVLGNSGGGGFGRAAATGIGAVAGSVIGNQ
                     VEANTSPVSEVPVRRCQTVVSYENRTIGYDVMYDYAGQRYSTRMARDPGQRFAVSVQP
                     ADASGAALPAPAAAAPVPQAGYDDAAAAPTYAAAPAAPVYYYQPPAYPYYYAPAPYFY
                     GAPAISFGFGYYGGWRGGRHWH"
     gene            complement(3715..4713)
                     /gene="gap"
                     /locus_tag="VNU48_06745"
     CDS             complement(3715..4713)
                     /gene="gap"
                     /locus_tag="VNU48_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWI11010.1"
                     /translation="MTIKIGINGFGRIGRMVFRAAVKNFKDVEVVGINDLLEPDYLAY
                     MLQYDSVHGRFKGSIAVDGNTLIVGGKKIRLTQERDPAALKWNEVGADIVVEATGLFL
                     TKETGQKHIDAGARKVIMSAPSKDDTPMFVFGVNDTRYKGEAIISNASCTTNCLAPVA
                     KVLHDKWGIKRGLMTTVHATTATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VIPELNKKLTGMSFRVPTSDVSVIDLTVELNSPASYAEICAEMKAQSAGAMKGVLGYT
                     EDKVVSTDFRGEAMTSVFDADAGIALDSTFVKVVSWYDNEWGYSNKVLEMVRVIAK"
BASE COUNT          696 a         1673 c         1681 g          766 t
ORIGIN      
        1 acgccggcca ccgcaatggc tcccgggcaa ttggccgggg tgccgaccgc gaggccctca
       61 ttgccggcgg cgacgacgac gacgacaccc cgggcgttga cctgctggat cgcgtcctgg
      121 taagccgcgc tgcaggcgcc gctcgcgccc aggctcatgt tgatgacccg ggcgcgcggc
      181 accgtcgtcg tggacccgct ctccaggctg gcggcccaca gcatcgcggc ctggatgtcg
      241 gagtcgaagc cgccgcactt gccgagcacg cgcagcggca acagcatgat gtcgcggccg
      301 gtgccggcca tgccgacgcc gttgttcgtc gccgcgccca ccaggccggc cgtctgcgtg
      361 ccgtgccagc tgctgttgcc gacatcgctg ctggtgcagc ccggcaccgc cgcgccgacg
      421 tcggccgacg tgacgtagtc gcccggatcc gaggcgtcgc tgtcgcgtgc ggtcccgtca
      481 ttggcggtga gcgtgttgct gatgaagtcg gagccggcca gcagcttgct gctcagatcg
      541 ggatggtcga agcgcacgcc ggtgtcgagc atcgcgacga cgatggccgg gttgccggtc
      601 gtgacgtccc acgccgcctg tgcgttgatc gccgagacca cgctggtggc gtcgacgatg
      661 gtcgcgctgg tcggcgcgcg caggtaccac tggccggtga cgggcgtcgt cggggcagcg
      721 aggttgttcg ggtacagcgg gtcgctgggg gccgcgagcg cgtgcatgcg accgtcgacg
      781 accgcgtatt ccacgtcgtc ctgcgcggcc agccggtcgg ccaggtcctg cgagctcatg
      841 ccccggccct tcagcacctg cgcgtgcgcg ccgatcgtgc gaccgtcgct cagcgccagg
      901 ccgagccggg ccgccagcgc ctgcgcgtgc tgcggcggcg cgccgtggcc ggcagcttct
      961 ccgggcagcg cacgcgtcag cgccgcgtct gccttgtact tgacgataac acgcgcctcg
     1021 gtctcggccg ccaccgccac ggcccgctgc ggcggcccac gctcggcgcc gcgcggcggc
     1081 ggcgccgcgt tgctggccac gcacgccagg gccagcacac agacgacccg cgcagcggct
     1141 tgacggagaa gtgtcttcat gagcggttca ccgggaggta ggcaccgtcg cggcacctgc
     1201 agacaggccc gtgcgcacgc agcgcgcggg agctggacaa cattcccgcg agtgtaggca
     1261 cacggcccgg cggcgcatcc gcgcaaaagc gcacggaaac gccgccgttt tcgagtcgtt
     1321 accgggtcag cagttgcggt gccgaaatgt cgccaacggc caggacgctg taggtcgcgt
     1381 tcgagttcag cgtgccggtc ccgctcgcgc ccagcaggcc ggtcaacggg cccgcggtcg
     1441 acgacgtgag gccgaagctg aacgtgttgg ccgtcccggc gttgttggtc gtgccgatca
     1501 gcgactggta gctcgaccac tgaccggctc cgatgttgat gcccaccggc gccgagttgg
     1561 ccgtcaaggt cagtgtgccc gcgctgccgg tgacgccgtt gatcagccgc agcctggtcg
     1621 cggtcgcgct ggtgggcggg cggttgtcgt cggcgatcag cgtggcggtc gaactcgacg
     1681 ggctgccgta gaccagcaga gtcgcgtcac tgccggccac caatgagcct ggcacctgga
     1741 ccgaattgcc gacgccgacg ttgacattca gcgaactggt cgcaggcacc agcgcgtaga
     1801 agccgaacgc cggcgcgacg ctgctgtcga tcgtgatggg ggcgctcaag gtgctgtcgc
     1861 tggccaatgc tgtcacggtc gcgccgcccg acaccgcggc cgccagacgc acgcgcgcgt
     1921 tcgggttggg taccgcggtg taggcgccct gctggaccag caacgcgccg ttgatcagtg
     1981 caccaccgga cgttggcgtc atcaacaccg tggcgacggt ctggccagcg agtgtgaccg
     2041 gcagactcat gcgaatgtcg gtcttgctac cggagccggt ggcgcagacg ttgtaggtgc
     2101 cggcgccctg cgtcaccgtg gtcgcggcca ccgacacgat cgcgccgaaa ttcgcggccg
     2161 gcgagatggt cgtcaagttc gcggtggtgc aggcgccggc cgtgatgtag acgtcgactg
     2221 cgcccgcctc gatggccgcg tcgtagatgc gcaaggtggt tacgcccgca gcgggcaggg
     2281 cgacgtcctc gccgatcgcg ggggtgaatt tcacgacgcc gccggactcg taggcgacca
     2341 ccgtgtagtg attgcccccg gtcagcgtgg gcacggcggt gacgagcgcg gtggcgccgc
     2401 cggcgtcgtt gacctgcagc gtgacactgc cggcagccgg cgcggcatag gcgctgacgg
     2461 taccgacggc gaccgccggg ggcacgatcg cggcattcac cagcaggtcg atcgacgcat
     2521 ggctgggcga gagcgtcgcg ttggcgacgc gcacgttcgc gctcgacgaa tcgccgccgc
     2581 cgctgccgca acccgacacc gccacggtcg ctgccgtcag gccgaccacc gaccaccaac
     2641 tgaaattctt cacgtctcga atccttgcac tgaatgtgtc cacgaacggg gccccgctca
     2701 cgcggcgggg gcccatgatt gggcccgcgg ggtgaagcaa gatgattgct gtggtgcgtc
     2761 cgatcgaaaa ttagtgtcaa cccgtcgacc ggccagcgcc gcaggcgcga tcataggccc
     2821 ctgcggacgc gcaaccgttc tttacgcggg attcacgccg cggatcggcc tgcgcgctat
     2881 gcttgaacga caagcagggg tttgccgctg caaggagagc atcatgcgca gagccattcg
     2941 cccggtgctg gctgccgtcg cggccgccgg cgccgccctc actgccccga ccgcattcgc
     3001 ggcggactac gcaaccgtcg tgtcgtcgac accggtcgcc gcatcggtcc cggtgcgccg
     3061 gcaggtctgc aacgacaccc agcaatacgt ccagcaggcg ccgagcggcg ccggcgcggt
     3121 ggtcggcgcg atcgccggcg gcgtgctcgg caacagcggc ggcggcggct tcggccgcgc
     3181 cgcggcgacc ggcatcggcg cggtcgcggg ctcggtgatc ggcaaccagg tcgaggccaa
     3241 cacgagccct gtcagcgagg tgccggtgcg ccgctgccag accgtcgtga gctatgagaa
     3301 ccgcaccatc ggctacgacg tgatgtacga ctacgccggc cagcgctaca gcacccgcat
     3361 ggcgcgcgac ccgggccagc gcttcgccgt cagcgtgcag ccggcggatg cgtcgggcgc
     3421 ggcgctgccg gcgccggccg ccgccgcgcc ggtgccgcag gccggctacg acgatgcagc
     3481 ggcagcgccg acctacgccg ccgcgcccgc ggcgccggtg tactactacc agccgccggc
     3541 ctacccctac tactacgcgc cggcaccgta tttctacggc gcgccggcga tcagcttcgg
     3601 cttcggctac tacggcggct ggcgcggcgg tcgccactgg cactgagcgg cgtcgacggc
     3661 gagcagaaga aaacgcgccc ctcgggcgcg tttttcatga gcgcggcgcc cgccctactt
     3721 cgcgatcacg cgcaccatct cgagcacctt gttcgagtag ccccattcgt tgtcgtacca
     3781 ggacacgacc ttgacgaagg tgctgtccag cgcgatgccg gcgtcggcat cgaacaccga
     3841 ggtcatcgcc tcgccgcgga agtcggtcga gacgaccttg tcctcggtgt agccgagcac
     3901 gcctttcatc gcccctgcgg actgcgcctt catctcggcg cagatctcgg cgtagctcgc
     3961 cgggctgttc agctcgacgg tcaggtcgat caccgacacg tcggacgtgg gcacgcggaa
     4021 cgacatgccg gtcagcttct tgttcagctc ggggatcacg acgccgaccg ccttggccgc
     4081 gccggtcgac gacgggatga tgttctcgag gatgccgcgg ccgccgcgcc agtccttgtt
     4141 gctcggcccg tcgaccgtct tctgcgtcgc ggtggtggcg tgcaccgtcg tcatcaggcc
     4201 gcgcttgatg ccccacttgt cgtgcagcac cttggccacc ggcgccaggc agttggtcgt
     4261 gcacgaggca ttcgagatga tcgcctcgcc tttgtagcgc gtgtcgttga cgccgaacac
     4321 gaacatcggc gtgtcgtcct tcgacggcgc cgacatgatg accttgcgcg cgccggcatc
     4381 gatgtgcttc tgaccggtct ccttggtcag gaacaggccg gtggcctcga cgacgatgtc
     4441 ggcgcccacc tcgttccact tcagcgccgc ggggtcgcgc tcctgcgtca ggcggatctt
     4501 cttgccgccc acgatcagcg tgttgccgtc gaccgcgatg ctgcccttga agcggccgtg
     4561 caccgagtcg tactgcagca tgtacgcgag gtagtcgggc tcgagcaggt cgttgatgcc
     4621 gacgacctcg acgtccttga agttcttgac ggcggcacgg aacaccatgc gcccgatgcg
     4681 gccgaagccg ttgatgccga tcttgatggt cattgctgtt ctccagggat gggtagtcta
     4741 agaaacctag tggtcgaccg tgaaggtcat ccgaaacgga tagctcatgc ggcagggctt
     4801 cccgctgcag acggcc
//
LOCUS       DATMJB010000308         5132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_749086, whole genome shotgun sequence.
ACCESSION   DATMJB010000308 DATMJB010000000
VERSION     DATMJB010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749086"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="VNU48_06750"
     CDS             complement(<1..319)
                     /locus_tag="VNU48_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M20"
                     /protein_id="HWI11011.1"
                     /translation="MNARDPLLPVQPDESSAIARFASRAWDEEIVPLVTNYIAVPAKS
                     PMFDADWAANGHIERVIRDAASWVESRRVAGLKLEVVRLEGRTPVIFFEVPATRAGGN
                     EKDA"
     gene            complement(371..1222)
                     /locus_tag="VNU48_06755"
     CDS             complement(371..1222)
                     /locus_tag="VNU48_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HWI11012.1"
                     /translation="MSTLTTSDGLKLQLREWPCDGARGTVLIVHGLGEHLGRYEHVAA
                     QLLRGGWHVVGYDQRGHGASEGPRGRLHHPDDLLRDLALVIDAVRVRLRGPLLLLGHS
                     MGGLVAARMVAAGLDDTRPPKWHRAVDALVLSSPALDLGLGGAQKLLLALLGRIAPNF
                     AVGNGLKPEWISRDPAVVEAYVDDPLVHDRVAPKLVRFIVDGGAFVRAHAARWRVPTL
                     LIYAGNDRCVAPAGSAAFAAAAPVDVVSTQAFPALYHEIFNEPEQASVFAVLGAWLAR
                     VPSRPGR"
     gene            complement(1274..2692)
                     /locus_tag="VNU48_06760"
     CDS             complement(1274..2692)
                     /locus_tag="VNU48_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWI11013.1"
                     /translation="MSKRAFARRAGGFGALAGALLLGGCMNLIPKYERPAAPVAPAYA
                     PELAPAGTAAAAGDIEWQRFFADPRLKRLIELALANNRDLRVAALNIEQARALYQVRR
                     ADELPTLGAGMTAQRQPGASGGLVNTYAVGVQVTGYELDFFGRVRALSQAALAQYLAT
                     DEARKTAQIALIAGVATTHVGILADDELLRVTRDTLKTREDSFRLTRLRFDNGASSEI
                     DLRQAELLLETARAALAQTQRQRALDQNALILLVGQPLPADLPAPLPLAAQQGVSDLP
                     AGLPSELLTRRPDLRAAEQQLLAANASIGAARAAFFPRIALTASAGTASTELSGLFKS
                     GSFALTGTATLLQPIFDAGRNQANLEVAKVNKDIAIAQYEKAIQTAFREVSDSLAGRA
                     TLGEQLRAQAAAANAAQIGFKLADLRYRNGVASYFDVLEAQRSLFAAQQATVQVQAAQ
                     VQNLVTLYKVLGGGWTEPRSTN"
     gene            complement(2685..>5132)
                     /locus_tag="VNU48_06765"
     CDS             complement(2685..>5132)
                     /locus_tag="VNU48_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWI11014.1"
                     /translation="DIVLRANADGSAVRLKDVARIELGGQSYATSARLNGKPSTGIGV
                     QLTPTGNALQTAKLIKARMEELQAYFPAGVKYSIPYDASRFVKISITEVVKTLVEAVI
                     LVFLVMYLFLQDFRYTLIPTIAVPVALLGAFGMMFVFGYSINVLTMFAMVLAVGILVD
                     DAIVVIENVERIMSEEGLSPRDATKKAMGQIQAALIGITVTLVSVFLPMAFFAGSVGN
                     IYRQFSLVMVSSMLFSVFLANSLTPALCATFLKPVEAGHKHGKRGFFGWFNRGFKATT
                     HGYEGWVARMLTRTWRFMALFAAIVAVVVLLFTRLPASFLPNEDQGYIITSVQLPPGA
                     TQARTLAVIQQAEKFFLSQPEVDKMISLLGFSFSGSGQNTALAFVPLKPWGERKGAQH
                     SAQALAGRAFGALSGVRDAFVFALSPPAIPELGTATGFTFRLQDRGSNGHAALLAARN
                     QMLGMAAQSKVLAGVRPDGLEDAPQLQIDVDRDRASALGVSFDSINSLLSTALGSAYV
                     NDFPNAGRLQRVVVQADAPDRMQPDDLLEMNVMNSAGKLVPLSAMATTRWITGPMQTI
                     RYNGYPTMRLAGDAAPGYSTGDAMNEMEALAAKLPAGFGFEWTGQSREERLSGSTAMI
                     LLAFSLLAVFLCLSALYESWSIPFSVLLAVPLGVLGAVLGASMRSLPNDVFFKVGLIT
                     VIGLSAKNAILIIEFAKDLHAEGMGLIEATLKAVHLRFRPILMTSIAFILGVLPLVLS
                     SGAGSASQRAIGTGVMGGMITATVLAVFFVPVFFVVVRSIFKGSERQRHLYAHDLNGN
                     KPAPPLAAAGAKEGPHE"
BASE COUNT          781 a         1789 c         1817 g          745 t
ORIGIN      
        1 cggcgtcttt ttcgttgcca ccggcccgcg tcgccggcac ctcgaagaag atcaccggcg
       61 tgcggccttc gaggcgcacg acctcgagct tcaggcccgc gacgcgtctg ctttcgaccc
      121 agctcgccgc atcgcggatc acgcgctcaa tgtggccgtt cgcggcccag tcggcgtcga
      181 acatcgggct cttcgcggga acggcgatgt agttcgtgac cagcgggacg atctcttcgt
      241 cccacgcccg gctcgcgaag cgcgcgatcg cgctggattc gtcgggctgc accggcagca
      301 gggggtcgcg tgcgttcatc tcggtctccg gccacggaag gccgcagtgt aggacgtcgg
      361 ccgttcctgc tcagcgcccg gggcgactcg gcacgcgcgc cagccacgcg cccaacaccg
      421 cgaacacgct ggcctgctcg ggctcgttga agatctcgtg gtacagcgcc ggaaaggcct
      481 gcgtgctgac cacgtcgacg ggtgcggccg cggcgaacgc agcactgccg gccggggcga
      541 cgcagcggtc gttgccggca tagatcagca gcgtcggcac gcgccagcgc gcggcgtgcg
      601 cgcgcacgaa cgcaccgccg tcgacgatga agcgcaccag cttcggcgcg acgcggtcgt
      661 gcaccagcgg gtcgtcgacg taggcctcga cgactgccgg gtcgcgcgag atccattcgg
      721 gcttgagccc gttgccgacc gcgaaattcg gcgcgatgcg gccgagcagc gccagcagca
      781 gcttctgcgc accgcccagc ccgaggtcga gcgccggcga cgacagcacc agcgcatcga
      841 cggcgcggtg ccacttcggc ggcctggtgt cgtcgagccc ggccgcgacc atgcgcgccg
      901 cgaccagccc gcccatgctg tggccgagca gcagcagcgg gccgcgcagg cgcacgcgca
      961 ccgcgtcgat caccagcgcg agatcgcgca gcaggtcgtc ggggtggtgc agccggccgc
     1021 gcgggccttc gctcgcgccg tggccgcgct ggtcgtagcc gacgacgtgc cagccgccgc
     1081 gcagcagctg cgccgccaca tgctcgtagc gcccgaggtg ctcgccaagc ccgtgcacga
     1141 tcagcaccgt gccgcgcgcg ccgtcgcaag gccactcgcg cagctgcagc ttcaggccgt
     1201 ccgaggtggt cagggtcgac atcggcgtgc agtgtaggct ggcggcttcg cgcagcgcac
     1261 cccggagaac acctcagttc gtcgagcgcg gctcggtcca gccgccgccg agcaccttgt
     1321 acagcgtcac caggttctgc acctgcgccg cctgcacctg caccgtcgcc tgctgcgcgg
     1381 cgaacaggct gcgctgcgct tcgagcacgt cgaagtagct ggccacgccg ttgcggtagc
     1441 gcaggtcggc cagcttgaag ccgatctgcg ccgcgttcgc tgcggccgcc tgcgcgcgca
     1501 gctgttcgcc cagcgtggcg cgcccggcga gcgaatccga aacctcgcgg aacgcggtct
     1561 ggatcgcctt ctcgtactgg gcgatcgcga tgtccttgtt gaccttcgcg acctcgaggt
     1621 tggcctggtt gcggccggcg tcgaagatcg gctgcaacaa ggtcgcggtg ccggtgagcg
     1681 cgaagctgcc gctcttgaac agacctgaca gctcggtgct ggcggtgccg gcactggcgg
     1741 tcagcgcgat gcgtgggaag aacgcggcgc gcgccgcgcc gatgctggcg ttcgcggcca
     1801 gcagctgctg ctcggccgcg cgcaggtcgg gccggcgcgt cagcagttcc gacggcagcc
     1861 cggcgggcag gtcgctcacg ccctgctgcg cggccagcgg cagcggcgcg ggcaggtcgg
     1921 cgggcaaggg ctggccgacg agcaggatca gcgcgttctg gtcgagcgcg cgctggcgct
     1981 gcgtctgcgc gagcgcggcg cgcgcggtct cgagcagcag ctcggcctgg cgcaggtcga
     2041 tctcgctcga ggcgccgttg tcgaagcgca gccgggtcag gcggaacgag tcctcgcgcg
     2101 tcttcagcgt gtcgcgcgtg acgcgcagca actcgtcgtc ggcgaggatg ccgacgtgcg
     2161 tcgtcgcgac accggcgatg agcgcgatct gcgccgtctt gcgtgcctcg tcggtggcga
     2221 ggtactgcgc cagcgcggcc tggctcaagg ctcgcacgcg gccgaagaag tcgagttcgt
     2281 agccggtcac ctgcacgccc accgcatagg tgttgacgag gccgccgctc gcgccgggct
     2341 ggcgctgcgc ggtcatgccg gcaccgagcg tgggcagctc gtcggcgcgc cgcacctggt
     2401 agagcgcgcg cgcctgctcg atgttcagcg ccgcgacgcg caggtcgcgg ttgttcgcga
     2461 gcgcgagttc gatcaggcgc ttcaggcgcg ggtcggcgaa gaagcgctgc cactcgatgt
     2521 cgcccgcggc cgcggccgtg ccggccggcg ccagctcggg cgcgtaggct ggcgccaccg
     2581 gcgcggccgg gcgctcgtac ttggggatca ggttcatgca gccgccgagc agcagcgcgc
     2641 cggcgagcgc gccgaatccg ccggcacggc gggcgaacgc gcgcttactc atggggcccc
     2701 tccttcgcgc ccgccgccgc gagcggcggt gccggcttgt tgccgttcag gtcgtgcgcg
     2761 tacaggtggc gctggcgttc gctgcccttg aagatgctgc gcacgacgac gaagaacacc
     2821 ggcacgaaga acaccgcgag cacggtggcg gtgatcatgc cgcccatcac gccggtgccg
     2881 atcgcgcgct ggctcgccga gcccgcgccc gacgacagca ccagcggcag cacgccgagg
     2941 atgaatgcga tcgaggtcat caggatcggc cggaagcgca ggtgcacggc tttcaaggtc
     3001 gcctcgatca ggcccatgcc ctcggcgtgc aggtctttcg cgaactcgat gatcaggatc
     3061 gcgttcttgg ccgacaggcc gatcacggtg atcagcccga ccttgaagaa cacgtcgttg
     3121 ggcaggcttc tcatcgacgc gccgagcacg gcgccgagca cgcccagcgg caccgcgagc
     3181 agcaccgaga acgggatcga ccagctctcg tacagcgccg acaggcacag gaacaccgcg
     3241 agcagcgaga acgcgagcag gatcatcgcg gtcgagcccg acaggcgctc ctcgcgcgac
     3301 tgaccggtcc actcgaagcc gaagcccgcg ggcagcttcg cggcgagcgc ctccatctcg
     3361 ttcatcgcgt cgccggtgct gtagccgggc gccgcgtcgc cggccaggcg catcgtcggg
     3421 tagccgttgt agcggatcgt ctgcatcggc ccggtgatcc atcgcgtcgt cgccatcgcc
     3481 gacagcggca cgagcttgcc ggcgctgttc atcacgttca tctcgagcag gtcgtcgggt
     3541 tgcatgcggt cgggtgcgtc ggcctgcacg acgacgcgct gcaggcggcc ggcgttcgga
     3601 aagtcgttca cgtaggccga gccgagcgcg gtcgagagca gcgagttgat gctgtcgaac
     3661 gagacgccga gtgcgctcgc gcggtcgcgg tcgacgtcga tctgcagctg cggcgcatcc
     3721 tcgagtccgt cggggcgcac gccggcgagc accttgctct gcgccgccat gcccagcatc
     3781 tggttgcgcg ccgcgagcaa cgcggcatgg ccgttgctgc cgcggtcctg caggcggaag
     3841 gtgaagccgg tcgcggtgcc gagctcggga atcgccggcg ggctgagcgc gaagacgaac
     3901 gcgtcacgca cacccgacag cgcgccgaac gcacgcccgg cgagcgcctg cgccgaatgc
     3961 tgagcgccct tgcgctcgcc ccagggcttg agcggcacga acgcgagcgc ggtgttctgg
     4021 ccgctgcccg agaagctgaa gccgagcagg ctgatcatct tgtcgacctc gggctggctc
     4081 aggaagaact tctcggcctg ctggatcacc gcgagcgtgc gcgcctgcgt cgcgcccggc
     4141 ggcagctgca cgctggtgat gatgtagccc tggtcctcgt tcggcagaaa cgacgccggc
     4201 aagcgtgtga acagcagcac cacgaccgcg acgatcgcgg cgaacagggc catgaagcgc
     4261 caggtgcgcg tcagcatccg cgcgacccag ccttcgtagc cgtgggtggt cgccttgaag
     4321 ccgcggttga accagccgaa gaagccgcgc ttgccgtgct tgtggccggc ctccaccggc
     4381 ttcaggaagg tcgcgcacag cgccggcgtc aacgagttcg cgaggaacac cgagaacagc
     4441 atcgagctga ccatcacgag cgagaactgg cggtagatgt tgcccaccga gccggcgaag
     4501 aacgccatcg gcaggaacac cgagaccagc gtcacggtga tgccgatcag cgcggcctgg
     4561 atctggccca tcgccttctt cgtcgcgtcg cgcgggctca ggccttcctc gctcatgatg
     4621 cgctcgacgt tctcgatcac gacgatcgca tcgtcgacca ggatgccgac cgcgagcacc
     4681 atcgcgaaca tcgtcagcac gttgatcgag tagccgaaca cgaacatcat gccgaacgca
     4741 ccgagcagcg cgaccggcac cgcgatcgtc gggatcagcg tgtagcggaa gtcctgcagg
     4801 aagagataca tcaccaggaa caccaggatc accgcctcca ccagggtctt gacgacctcg
     4861 gtgatcgaga tcttgacgaa gcgcgaggcg tcgtacggaa tcgaatactt cacgccggcc
     4921 ggaaagtacg cctgcagttc ctccatgcgc gccttgatca gcttggcggt ctgcagcgca
     4981 ttgccggtgg gcgtgagctg cacgccgatg ccggtcgagg gcttgccgtt caggcgcgcc
     5041 gaggtcgcgt agctctggcc gccgagctcg atgcgggcga catctttcag gcgcacggcc
     5101 gagccgtcag cattcgcgcg cagcacgatg tc
//
LOCUS       DATMJB010000309         4866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_259911, whole genome shotgun sequence.
ACCESSION   DATMJB010000309 DATMJB010000000
VERSION     DATMJB010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259911"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1070)
                     /locus_tag="VNU48_06770"
     CDS             complement(60..1070)
                     /locus_tag="VNU48_06770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWI11015.1"
                     /translation="MSAAPGTRPAADGARLGVVVIGRNEGERLRRCLSSVLPQTPTVV
                     YVDSGSSDGSFEMAAALGASAHALDLRQPFTAARARNEGVRRLQALAPALEFIQFVDG
                     DCEVAPQWMGTAVEFLDRHPDVAAVCGRRRERHPERSVYNLLCDLEWDTPIGESKACG
                     GDVLMRAAALRQVVGYRDELIAGEEPELCVRLRAKGWRVWRLDAEMTLHDAAMTHFGQ
                     WWRRCMRTGFAYAEGVRLHGAAPERHWVRESRSAWVWGAGIPLALTIAALAVGAAAGW
                     GLLLYPLQVARLAWRFSGTAGKRWARAFFLVAGKFPEALGQMKSWLVHARHRRAVLIE
                     YK"
     gene            complement(1067..1576)
                     /locus_tag="VNU48_06775"
     CDS             complement(1067..1576)
                     /locus_tag="VNU48_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HWI11016.1"
                     /translation="MFSNIRQDLRAHGGHFGAQGFWVMVVYRFGRWRYGVRPVLLRKC
                     FSLVYHVLYKLVQIATGIELPCEAEVGRNFVIDHFGGIVVSGFARFGDNCRIRNGVVV
                     GLARIDDPCAPVLGDNVDIGAGAKLLGRIRIGNNVLIGANAVVTRDVPDDSIAVGVPA
                     VIKPRRPAG"
     gene            complement(1597..2562)
                     /locus_tag="VNU48_06780"
     CDS             complement(1597..2562)
                     /locus_tag="VNU48_06780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWI11017.1"
                     /translation="MNRDDAIDVTALIVNYNTKELLRPCIDALRASASGLRLQIVIVD
                     NASRDGSPEWLRAEFGDCDLIFNTSNVGFGRANNQALALARGRHVLLLNTDAFVRPDS
                     IGNTVRFLDAHPRCGLLGVRLVGLDGTLQPSCRYFPTPWNEFLVRAGLSRLFPSTRLV
                     DDMAWDHASARECDWVPGCYYLVRKIVVDQVGLFDPRFFLYYEEVDHCRAVKAAGWSV
                     MYCPDTTVVHVGGQSAQKDAEVTSAGRQISALQIESALLYYRKHHGRLGLWASALLAS
                     LGDALLALKWLLRGRALRGVGPYWTHAATTWALLRRTAWGRRATR"
     gene            complement(2606..3733)
                     /locus_tag="VNU48_06785"
     CDS             complement(2606..3733)
                     /locus_tag="VNU48_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWI11018.1"
                     /translation="MTLIANAKTIARKIEWRLSRRQRVVRSLDELSGEVAALLVTDSH
                     ELSQTQVYPFHAFAGDIEGAIGGRLHEIDLECVAAQAERPPLPGVRWVGFQAVLNCPV
                     DVLLRQVQQLRRLFPNAALVYFDWYAPLDLRHAPALDPWVTHYVKKQTFSDLRRYSDT
                     TIGDTNLMDYYSRRYGLQEPTTQHEFPLGFERKLTMGTNFCVSPHMLDRFMTRPPERA
                     RHHDVHARIAVRGTDWYQRMRQEALDLARSVKGVRTVSEGRVSQRQFFDELARSKICF
                     SPFGYGEVCWRDFEAVFSGALLLKPRMDHVRMEPDIFVDGQTYVALEWDGSDYEEKVR
                     HYLSHEAERLRIVEQAYDVVQRYLRERPFVARYLRLLGHAT"
     gene            complement(3730..>4866)
                     /locus_tag="VNU48_06790"
     CDS             complement(3730..>4866)
                     /locus_tag="VNU48_06790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWI11019.1"
                     /translation="VFTGKVDASVTDPHVHHVRRGWWGKMARRLARGLFSTERDIFEW
                     GAVIARHMLEVHRRDPIDVIEIEESFGWSADVSRITSIPVLVKLHGPAFLSLIDEELS
                     TPFGRERVEREGRALALATAIASPSSLTLTQTLERYRLAPRIQQHVVNPLAMDERTPV
                     WRLEACDPKTILFVGRFDKRKGADVVLRAFELLLRERPELRLVFVGPDSGLSQPDGTR
                     IHFEAFRATVFPEALRGRVEFRGRMANRDIAALRVQAMVTVIASRWENQGYTALEAMF
                     QGCPVVVSDAGGSPESVVDGRTGLLARSEDAGHFASRLRAMIDDPQAAAAMGAAARRH
                     VIETHSAAKVASESLLLYERLIALVRSNIHHGGGDRSTTRARAS"
BASE COUNT          819 a         1683 c         1658 g          706 t
ORIGIN      
        1 cttcgggtac tggttgatca ggtacgcgac acgccgcggc aaggtggtgg gcacggcgtt
       61 catttgtatt cgatcagcac cgcgcgccga tggcgggcgt gcaccagcca cgatttcatc
      121 tggccgagcg cctcggggaa cttgcccgcg acaaggaaga acgcgcgcgc ccagcgcttg
      181 ccggcagtgc ccgaaaagcg ccacgcgagc cgggccacct gcagcgggta gagcagcagc
      241 ccccaccctg ccgccgcacc caccgcgagc gccgcgatgg tcagcgccag cgggatcccg
      301 gcgccccaga cccaggcgct gcgcgactcc cgcacccagt gccgctcggg cgccgcaccg
      361 tgcaggcgca ccccttcggc gtacgcgaaa ccggtgcgca tgcagcgccg ccaccactgg
      421 ccgaagtgcg tcatcgcggc gtcgtgcaag gtcatttcgg catcgagccg ccagacccgc
      481 cagccctttg cgcgcaggcg cacgcacagc tccggctcct cgccggcgat cagctcgtcg
      541 cggtagccga cgacctggcg cagcgccgcg gcgcgcatca gcacgtcgcc gccgcaggcc
      601 ttggattcgc cgatcggcgt gtcccattcg aggtcgcaaa gcaggttgta gaccgagcgc
      661 tcggggtggc gctcgcgccg ccgcccgcac accgcggcga catccgggtg gcgatcgagg
      721 aattcaaccg cggtgcccat ccactgcggc gcgacctcgc aatcgccgtc gacgaactgg
      781 atgaattcca gcgccggggc cagtgcctgc aggcggcgca cgccctcgtt gcgcgcgcgc
      841 gccgcggtga agggctggcg cagatcgagc gcatgggccg atgcgcccag cgccgcggcc
      901 atctcgaacg aaccgtcgct ggaacccgaa tcgacgtaga cgacggtcgg ggtctgcggc
      961 agcaccgagc tcaggcaacg acgcagccgc tcgccttcgt tgcggccgat caccaccacg
     1021 cccaggcggg cgccatcggc ggcagggcgg gtgccgggtg ccgcgctcat ccggccgggc
     1081 gcctgggctt gatcacggcc ggcacgccga ccgcgatgct gtcatcgggc acatcgcggg
     1141 tgaccacggc gttcgcgccg atcagcacgt tgttgccgat gcggatgcgg cccagcagct
     1201 tcgcgccggc gccgatgtcg acgttgtcgc cgagcaccgg cgcgcacggg tcgtcgatgc
     1261 gcgccagccc gacgaccaca ccgttgcgga tgcggcagtt gtcgccgaag cgcgcgaagc
     1321 cgctgacgac gatgccaccg aagtggtcga tcacgaagtt gcgcccgacc tcggcctcgc
     1381 agggcagctc gatgccggtg gcgatctgca ccagcttgta cagcacgtgg tacacgagcg
     1441 agaagcattt gcgcagcagc accgggcgca caccgtagcg ccagcggccg aagcggtaca
     1501 cgaccatgac ccagaaaccc tgggcaccga agtggccgcc gtgcgcgcgc aggtcttgtc
     1561 ggatgttgga gaacatggtg ggcagccggg tcagcgtcag cgggtcgcgc gtcggcccca
     1621 ggcggtcctg cgcagcagtg cccaggtcgt cgccgcatgg gtccagtatg ggccgacgcc
     1681 ccgcagcgcc cggccacgca gcagccattt cagcgcgagc agcgcatcgc ccagcgacgc
     1741 gagcagcgcg ctcgcccata ggcccagacg accgtggtgc ttacggtaat agagcaacgc
     1801 gctttcgatc tgcagcgccg agatctgccg ccccgcggac gtgacctcgg catccttctg
     1861 tgcgctctgt ccgccgacgt gaaccacggt cgtgtcggga cagtacatca cactccagcc
     1921 ggcagccttg acggcacggc agtggtcgac ctcctcgtag tacagaaaga agcgcggatc
     1981 gaagaggccg acctggtcga cgacgatctt gcgcacgagg tagtagcagc caggcaccca
     2041 atcgcactcg cgtgccgacg cgtggtccca ggccatgtcg tcgacgaggc gcgtgctggg
     2101 gaacagccgg ctcagcccgg cacgcacgag gaactcgttc cacggcgtgg gaaagtagcg
     2161 acacgagggt tgcagggttc cgtcgagccc caccaggcgc acgccgagca ggccgcagcg
     2221 cggatgggcg tcgagaaagc gcacggtgtt gccgatgctg tcgggccgaa cgaaggcatc
     2281 ggtgttgagg agcagcacat gtcggccccg cgccagcgcg agcgcctggt tattggcgcg
     2341 accgaagccg acgttgctcg tgttgaagat cagatcgcag tcgccgaact cggcgcgcag
     2401 ccattcgggc gaaccatcgc gcgaggcgtt gtcgacgatg acgatctgca ggcgcaagcc
     2461 gctcgcgctg gcacgcagcg cgtcgatgca agggcgcagc agctccttgg tgttgtagtt
     2521 gacgatgagg gccgtcacgt cgatggcgtc gtcgcggttc atctgcgagc gccgccgatc
     2581 agcgaaacgg acgaggcccg cgcgctcacg tcgcgtgccc gagcagccgc aggtatcgag
     2641 cgacgaaggg gcgctcgcgc aggtagcgct ggaccacgtc gtaggcctgc tcgacgatgc
     2701 gcagccgctc ggcctcgtgg ctcaggtagt ggcgcacctt ctcttcgtag tcggagccgt
     2761 cccactcgag ggcgacgtag gtctggccgt cgacgaagat gtccggctcc atgcgcacgt
     2821 ggtccatgcg cggcttcagg agcaacgcgc cggagaacac cgcctcgaag tcgcgccagc
     2881 aaacctcgcc gtagccgaac gggctgaagc agatcttgct gcgtgcgagt tcgtcgaaga
     2941 actgccgttg ggaaacgcgg ccctcggaga cggtgcgcac gcccttgacc gaacgcgcca
     3001 ggtcgagcgc ctcctggcgc atgcgctggt accagtcggt gccgcgcacg gcgatgcgag
     3061 cgtgcacgtc gtggtgcctg gcgcgctccg gcgggcgtgt catgaagcga tcgagcatgt
     3121 gcggcgagac gcagaagttc gtgcccattg tgagcttgcg ctcgaagccg agcgggaact
     3181 cgtgctgcgt ggtgggctcc tgcagcccgt agcggcggct gtagtagtcc atcaggttgg
     3241 tgtcgccgat ggtcgtatcg gaatagcgcc gcaaatcgct gaaggtctgc ttcttcacat
     3301 agtgcgtgac ccaaggatcg agcgccggcg cgtgtcgcag gtcgagcggt gcgtaccagt
     3361 cgaagtacac gagcgcggcg ttcggaaaca agcgcctcaa ctgctggacc tgccgcagca
     3421 gcacgtcgac cggacaattg agcacggcct ggaaaccgac ccagcgcacc ccgggcagcg
     3481 gcgggcgctc ggcctgcgcc gcgacgcact cgaggtcgat ctcgtgcagc cggccgccga
     3541 tcgcgccttc gatgtcgccc gcgaaagcgt ggaaggggta gacctgggtc tgcgacagct
     3601 catgactgtc cgtcaccagc aaggccgcga cctcgccgga cagttcgtcg agcgagcgca
     3661 cgacccgctg gcggcgcgaa agcctccatt cgatcttgcg cgcgatcgtc ttcgcgttcg
     3721 cgatcagcgt catgacgcgc gcgcccttgt cgttgaccga tcgccgccgc catggtgaat
     3781 gttcgagcgg accagcgcga tcaagcgctc gtacagcaac agcgactcgc tcgcgacctt
     3841 ggccgcggaa tgggtctcga tcacgtgacg gcgggccgca gcgcccatcg cggcggccgc
     3901 ttgcgggtcg tcgatcatcg cgcgcaggcg cgacgcgaag tgccctgcgt cctccgagcg
     3961 cgccagcagg ccggtgcggc cgtcgacaac actctcgggg cttccgcccg cgtcgctgac
     4021 caccacgggg cagccttgaa acatcgcctc cagcgcggtg tacccctggt tttcccagcg
     4081 cgaggcgatc acggtcacca tcgcctggac acgcagcgcg gcgatgtcgc ggttcgccat
     4141 ccgcccccgg aactcgaccc gcccgcgcag cgcctcgggg aagaccgtcg cgcgaaacgc
     4201 ctcgaagtgg atgcgcgtgc catcgggttg cgacaagcca gaatccggcc cgacgaacac
     4261 gagcctgagc tccgggcgtt cacgcagcag gagttcgaac gcacgcagaa ccacgtcggc
     4321 ccccttgcgc ttgtcgaagc ggccgacgaa caggatcgtc ttcgggtcgc aggcctccag
     4381 ccgccacacg ggtgtgcgct cgtccatggc cagcgggttg acgacatgct gctggatgcg
     4441 gggggccaat cggtaacgtt cgagcgtctg cgtcagcgtc aggctggagg gcgacgcgat
     4501 cgccgtggcc agcgccagcg cccgcccctc gcgttcgacc cgttcgcggc cgaagggcgt
     4561 actcagttct tcgtcgatca gcgacaggaa cgccggaccg tgcagcttca ccagcacggg
     4621 gatcgacgtg atgcggctca catcggccga ccaaccgaac gattcctcga tctcgatgac
     4681 gtcgatcgga tcgcgacgat gcacctcgag catgtgccgg gcaatgaccg cgccccattc
     4741 gaagatgtcg cgctcggtcg agaacagccc tctggcgagc cgccgcgcca tcttccccca
     4801 ccacccacgg cgaacatgat ggacgtgggg atcggtcacc gaagcgtcga ccttgcccgt
     4861 gaacac
//
LOCUS       DATMJB010000310         2403 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_783768, whole genome shotgun sequence.
ACCESSION   DATMJB010000310 DATMJB010000000
VERSION     DATMJB010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783768"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="VNU48_06795"
     CDS             complement(<1..1422)
                     /locus_tag="VNU48_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HWI11020.1"
                     /translation="MTAATGATPSVYQGYQIIRRNGAVVSFEPNKIAVALMKAFLAVH
                     GTQGAASASVRETVDGLTESVVRALLRSRPGGGTFHIEDVQDQVELGLMRGGHHEVAR
                     AYVLYRERRSQERAKQGQPAVAAAEPVLTVIDRGQRVPLDFARLRTVIESACAGLGAE
                     VRAEPILAETKRNLYDGVPIDEVHKASILAARTLIEKDPGYTRATARLLMHTIRRDIL
                     GEETLQADMRTRYAEYFPGFIKKGVQAELLDEKLLQFDLARLGAALNADRDLAFDYLG
                     LQTLYDRYFLHIDEQRIELPQAFYMRVAMGLALNEIDREARAIEFYEVLSSFDFMSST
                     PTLFNAGTRRSQLSSCYLTTVADDLDGIYEALKENALLSKFAGGLGNDWTNVRALGSH
                     IKGTNGKSQGVVPFLKVVNDTAVAVNQGGKRKGAVCAYLESWHLDLEEFLELRKNTGD
                     DRRRTHDMNTANWIPDLFMKRVMD"
     gene            complement(1857..>2403)
                     /gene="ampD"
                     /locus_tag="VNU48_06800"
     CDS             complement(1857..>2403)
                     /gene="ampD"
                     /locus_tag="VNU48_06800"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HWI11021.1"
                     /translation="SPNFGPRPEGAAVDLVLVHSISLPPGEYGGDAIEKLFTNRLDWD
                     AHPYYRQIRGLEVSAHFLVRRGGELLQFVSCDARAWHAGASSWRGRTDCNDFSIGIEL
                     EGLEGGRFEAAQYRALARLIGTVMQRYPIVAVAGHEHVAPGRKNDPGAGFDWLRLQAL
                     LRLPARYFPEGVVPPHGSDRA"
BASE COUNT          373 a          828 c          804 g          398 t
ORIGIN      
        1 gtccatcacg cgcttcatga acagatcggg gatccagttc gcggtgttca tgtcgtgcgt
       61 gcggcggcgg tcgtcgccgg tgttctttcg cagctcgagg aactcttcca ggtccaggtg
      121 ccagctttcc aggtaggcgc agaccgcgcc cttgcgcttg ccaccctggt tcaccgcgac
      181 ggccgtgtcg ttgacgacct tcaggaacgg caccacgccc tggctcttgc cgttcgtgcc
      241 cttgatgtgg ctccccagcg cgcgcacgtt ggtccagtcg ttgcccaggc cgccggcgaa
      301 cttgctcagc agcgcgtttt ccttcagcgc ctcgtagatg ccgtcgaggt cgtcggccac
      361 cgtcgtcagg tagcagctcg acaattgcga gcgccgcgta ccggcgttga acagcgtcgg
      421 cgtcgagctc atgaagtcga agctggacag cacctcgtag aactcgatcg cacgcgcctc
      481 gcggtcgatc tcgttcagcg ccaggcccat cgcgacgcgc atgtagaacg cctgcggcag
      541 ttcgatgcgc tgctcgtcga tgtgcaaaaa gtagcggtcg tacagcgttt gcagaccgag
      601 gtagtcgaac gccagatcgc ggtcggcgtt caacgccgcg ccgagcctgg ccaggtcgaa
      661 ctgcagcagc ttctcgtcga gcagttcggc ctgcacgccc ttcttgatga agccgggaaa
      721 gtactcggcg tagcgcgtgc gcatgtcggc ctgcagcgtc tcctcgccga ggatgtcgcg
      781 gcggatggtg tgcatcagca gccgcgcggt ggcgcgcgtg tagcccgggt ccttctcgat
      841 cagcgtgcgc gccgccagga tcgacgcctt gtgcacttcg tcgatcggca cgccgtcgta
      901 cagattgcgc ttggtctcgg ccaggatcgg ctcggccctc acctccgcac cgagcccggc
      961 gcaggccgat tcgatcacgg tgcgcagccg cgcgaaatcg agcggcacgc gctggccgcg
     1021 gtcgatcacg gtcagcaccg gctcggccgc ggccacggcc ggctggcctt gcttggcgcg
     1081 ctcctgcgaa cggcgctcgc ggtacagcac gtaggcgcgc gccacttcgt ggtgaccgcc
     1141 gcgcatcagg ccgagctcga cctggtcctg cacgtcctcg atgtggaagg tgccaccgcc
     1201 cggacgcgaa cgcagcaacg cgcgcaccac cgactcggtc aggccgtcga ccgtctcgcg
     1261 cacgctcgcc gacgccgcgc cttgcgtgcc gtgcaccgcg agaaaggcct tcatcagcgc
     1321 cacggcgatc ttgttcggtt cgaacgacac caccgcaccg ttgcggcgga tgatctggta
     1381 gccctggtac accgaaggcg tcgcgccggt ggcggcggtc atcgggcgct gcgcggcagc
     1441 cggcgtggtg gcggtgggta caacttgcat gaggggccct cgttggtctg atgttgtcgg
     1501 gttcacggag ttcctggttt caacgcgtcg cgctgggcgg ccggtgactc gggcgggcgg
     1561 tcgacgcagg ctcgaaccag aggcggcggg cgtctcgaaa cgatgcagga agcaacgaaa
     1621 tgagcgatga agaatagcat gagtggacac tatatctggg gtccgaaggc tccacaagca
     1681 ctacggatag tgtgtcgacc cgcccgctcg acggtgcggc agagcgcttg gggcagcgtg
     1741 cgtggcggtg caagcgccgc gccggcgaca cttcgttgtg cgcgcccgcg ccacgcttgg
     1801 catcgcgccg aatttgccgc gccagaccgc gccgattcga gcgctcggcc gcgcgctcag
     1861 gcgcgatccg atccgtgcgg cggcaccaca ccctcgggaa agtaccgtgc cggcaagcgc
     1921 agcagcgcct gcagccgcag ccagtcgaag cccgcacccg gatcgttctt gcggcccggc
     1981 gcgacatgct cgtggcccgc caccgcaacg atcgggtagc gttgcatcac cgtgccgatc
     2041 agccgcgcga gtgcacggta ctgcgccgcc tcgaagcgcc cgccttccag gccttcgagc
     2101 tcgatgccga tcgagaagtc attgcagtcg gtgcgaccgc gccagctcga cgccccggca
     2161 tgccaggcgc gcgcgtcgca cgagacgaac tgcagcagct cgccgccgcg ccgcaccaga
     2221 aaatgggccg agacctcgag cccgcgaatc tgccggtagt acggatgcgc gtcccagtcg
     2281 agccggttcg tgaacaactt ctcgatcgcg tcgccgccgt actcgcccgg cggcaggctg
     2341 atcgaatgca ccagcaccag atcgaccgcc gcgccttcgg gccgcgggcc gaaattcggc
     2401 gac
//
LOCUS       DATMJB010000311         3046 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_318897, whole genome shotgun sequence.
ACCESSION   DATMJB010000311 DATMJB010000000
VERSION     DATMJB010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318897"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1619)
                     /locus_tag="VNU48_06805"
     CDS             complement(42..1619)
                     /locus_tag="VNU48_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWI11022.1"
                     /translation="MVIYQIYPRSFQDSNGDGIGDLPGIITRLGHIKSLGVDMIWISP
                     VFKSPMRDFGYDVSDYRSVDPIFGTLSDLRRLIDEARQLDMGVMLDMVVSHTSDQHPW
                     FEASRRRADGKNDWYLWADALPDGAPPNNWLSVFGGDAWNWDATRRQYYFHTFLDSQP
                     DLNFHHPAVQQQVLAEMRYWLEFGVAGFRFDACNHYFQDRQLRSNPPAPGAHTHALHP
                     YGFQQHLYDKNRPEVPGFLARVRELLDRYGAYSVAEVGGSDALQLAIDYVAPGRLHSA
                     YSFDLLRSTHSAAYVRGVLERLDADLPGPGVACHALSNHDKPRVATRWGEGRAPDAVA
                     RQMLALLACLKGTICLYQGEELGLPEADVPFERLQDPYGRRFWPEFKGRDGCRTPLPW
                     DDGPHGGFSSAEPWLPLAPTHLGLNVVAQEADPDSVLHFARRAFALRRERPALHAGNL
                     AFDSGSADATALGLLRFERRAPGSAVVCLFNLGDEALAVAAPPALERILLSGAVRRTE
                     AQTLLECNGFLIAELPG"
     gene            2094..>3046
                     /locus_tag="VNU48_06810"
     CDS             2094..>3046
                     /locus_tag="VNU48_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWI11023.1"
                     /translation="MSDFDAHLFREGTHARLYDKLGCHLFGNDGGARFAVWAPNAAAV
                     SVIGDWNSWQEGASPLAPRADGSGIWEGRFAQVRHGQAYKYRILSTHDGHVADKADPF
                     AVWAEEPPATASRAWDLDYAWDDAAWMATRAARNALDAPVAIYEMHAGSWRRRDGGFI
                     GYKELAHALADHVLAMGFTHVELMPITEHPFYGSWGYQTTGYFAPTARYGTPQDFMYF
                     VDHLHQRGIGVLLDWVPSHFPTDEHGLQYFDGTHLYEHADPRQGFHPDWNSSIFNYGR
                     NEVRSFLISNALFWVEEYHIDGLRVDAVASMLYLDYSREAG"
BASE COUNT          512 a         1017 c         1038 g          479 t
ORIGIN      
        1 caagtgtcca ccccggcaaa ttcgaccaga atgcggtacc gtcaacccgg caattccgcg
       61 atcaggaacc cgttgcattc gagcagcgtt tgcgcttcgg tccggcgaac cgcgccggac
      121 aacaggattc gttcgagcgc cggcggcgcg gccactgcca gggcttcgtc gccgaggttg
      181 aacaggcaga ccacggcact gccgggcgca cggcgttcga agcgcagcag gccaagcgcg
      241 gtggcgtcgg ccgatccgct gtcgaaggcg agattgccgg cgtgcagcgc cggccgttcg
      301 cggcgcagcg cgaaagcccg gcgtgcgaag tgaagcaccg agtccgggtc ggcttcctgt
      361 gcgacgacgt tcaggcccag gtgggtgggc gcgagcggca accacggttc ggcgctgctg
      421 aacccgccgt gcgggccgtc gtcccagggc agcggcgtgc ggcagccgtc gcggcccttg
      481 aactcgggcc agaagcgccg accgtacgga tcctgcaggc gctcgaatgg cacgtcggcc
      541 tcgggcagcc cgagctcctc gccctggtac aggcagatcg ttcccttcaa gcaggccagc
      601 agcgccagca tctggcgcgc gacggcatcg ggcgcgcgac cttcacccca acgcgtggcg
      661 acgcgcggct tgtcgtggtt gctcagcgcg tggcaggcga caccggggcc gggcaggtcc
      721 gcgtccagcc gttcgagcac gccgcgcacg taggcggcgc tgtgcgtgct gcgcagcagg
      781 tcgaagctgt aggcactgtg caggcggccg ggcgcaacgt agtcgatcgc cagctggagg
      841 gcgtcgctgc cgccgacctc ggccaccgaa taggcgccgt agcggtcgag cagctcgcgc
      901 acgcgagcga ggaagcccgg tacttcgggg cggttcttgt cgtacaggtg ctgctgaaac
      961 ccgtagggat gcagcgcgtg cgtgtgggcg cccggtgcgg gcgggttgct gcgcagctgc
     1021 cggtcctgga agtagtggtt gcaggcatcg aaacggaagc cggccacgcc gaactcgagc
     1081 cagtagcgca tctcggccag cacctgctgc tggaccgcgg ggtgatgaaa attcaggtcg
     1141 ggttggctgt cgagaaaggt gtggaaatag tactggcgcc gcgtcgcgtc ccagttccag
     1201 gcatcgccgc cgaacaccga gagccagttg ttcggcggcg cgccgtcagg caaggcgtcg
     1261 gcccacaggt accagtcgtt cttgccgtct gcgcgccgac ggctcgcctc gaaccagggg
     1321 tgctgatccg aagtgtgcga aaccaccatg tcgagcatca cgcccatgtc gagttgccgc
     1381 gcctcgtcga tcaggcggcg caagtcgctc agcgtgccga agatcgggtc gacgctgcga
     1441 tagtcggaca cgtcgtagcc gaagtcgcgc atcggcgact tgaataccgg gctgatccag
     1501 atcatgtcga cgcccaggct cttgatgtgc ccgaggcgcg tgatgatgcc gggcaggtcg
     1561 ccgatgccgt cgccgttgct gtcctgaaag ctgcgcgggt agatctgata gatgaccatg
     1621 cgaaacattc tgcgggagga ttccgtgcag gaccaggacc agggcctgtt aacgctatcg
     1681 gaggactcgc gtgactcacc aaacagccgc cagcaaggcg caaaccacag ccatagcggc
     1741 ggctatggcg aggatttgca acgccgctgg cggctgtttg gtgagatcac ccttcgggcc
     1801 gggccgccac aggccgcagg gcgtcgttgc tcactcgttg tgtgccgaag gcacacggcc
     1861 tcgttcgcgc ctagccctgc ggcctgtggc ggcccggcgc gagccctccc atagcgttaa
     1921 caggccctag gacgattcct acgttctttc gaccgatcca cccactccat ccgcgcgcgg
     1981 cttttgcgat aaccgacgct gcatcgcatg gggagcccgt gaacccggtc aagaagaaac
     2041 aagacgcgcc gcccaagtcc cgcagcaagg cgccggatgt cgtcggcccc gagctgagcg
     2101 acttcgacgc gcacctgttc cgcgaaggca cgcatgcgcg gctgtacgac aagctcggct
     2161 gccacctgtt cggcaacgac ggcggcgcgc gcttcgcggt gtgggcgccg aacgcagccg
     2221 cggtgtcggt catcggcgac tggaacagct ggcaggaagg cgcgtcgccg ctcgcgccgc
     2281 gtgccgacgg cagcggcatc tgggagggcc gattcgcaca ggtgcgccac ggccaggcct
     2341 acaagtaccg catcctgtcg acccatgacg ggcacgtcgc cgacaaggcc gacccgttcg
     2401 cggtctgggc cgaagaacca ccggcgacgg cgtcgcgcgc ctgggacctg gactacgcgt
     2461 gggacgatgc cgcgtggatg gccacgcgcg cggcgcgcaa cgcgctcgat gcgccggtcg
     2521 cgatctacga gatgcacgcc ggttcatggc gccgtcgcga tggtggtttc atcggttaca
     2581 aggagctggc ccatgcgctg gccgaccacg tgctcgcgat gggcttcacg cacgtcgagc
     2641 tgatgccgat caccgagcac ccgttctacg gttcatgggg ctaccagacc accggctact
     2701 tcgccccgac cgcgcgctac ggcacgccgc aggacttcat gtacttcgtc gaccacctgc
     2761 accagcgcgg catcggcgtg ctgctcgact gggtgccctc gcacttcccg accgacgaac
     2821 acggcctgca gtacttcgac ggcacgcatc tgtacgaaca cgccgacccg cgccagggct
     2881 tccaccccga ctggaactcg agcatcttca actacggccg caacgaggtg cgcagcttcc
     2941 tgatctcgaa tgccctgttc tgggtcgagg agtaccacat cgacggttta cgcgtggatg
     3001 cggttgcctc gatgctctac ctggactact cacgtgaggc gggcca
//
LOCUS       DATMJB010000312         3392 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_521765, whole genome shotgun sequence.
ACCESSION   DATMJB010000312 DATMJB010000000
VERSION     DATMJB010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521765"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VNU48_06815"
     CDS             <1..666
                     /locus_tag="VNU48_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HWI11024.1"
                     /translation="GDALEIAITLEPGAHALLTTPGATRFYRSTGAAALQSLRATLAR
                     DARLEWLPLETIAYSGCSAANRMRFELAPGAEMIGWDLLALGLPASGQPFVRGRFTQT
                     VELPGRWVEHGVIDASDACLLDSPLGWAGRRVLATLWFAAGSALAPTRRDALLGAARD
                     AAEADALRASAGATAVHDGVVVLRALAPRVEPAMALLTRTWSAWREIAWQLPACAPRV
                     WRT"
     gene            796..1107
                     /locus_tag="VNU48_06820"
     CDS             796..1107
                     /locus_tag="VNU48_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11025.1"
                     /translation="MRPAHLLVVGTALLLCTQVHAMQRCRIDGRLVYQASPCPEATQV
                     APRSAELPHTLAAVDADVPVKRTMAEVMREREAAIRARPTASDSRPDGAKILRDRMGA
                     L"
     gene            1171..1644
                     /locus_tag="VNU48_06825"
     CDS             1171..1644
                     /locus_tag="VNU48_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HWI11026.1"
                     /translation="MTMKHAIFLAGVLWLSMAQAADIYQWVDESGRTHLSDAVPEKYK
                     HSAKRVDSRLFEVSPQQRAEAEARAAREREQAMKGAADAPVGARGVAAPASAATPASA
                     PSKASDCATLRRQYAQSQECFAPFMNTNGSIKPGAFEKCTQLPDPSPKCGPEPSP"
     gene            complement(1660..2334)
                     /locus_tag="VNU48_06830"
     CDS             complement(1660..2334)
                     /locus_tag="VNU48_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWI11027.1"
                     /translation="MITVHHLNNSRSQRVLWLLEELGVPYEIQKYQRDPQTMLAPPAL
                     MKVHPLGKSPVITDGDTTVAETGAIVEYLLERYGNGRLVPAAGSPQRLRWTYWLHFAE
                     GSAMPPLLLKLVFERIKVTPMPFFAKPIARGLANKVLAAMVDPNLKRQLDFMEAELGN
                     SEWFAGNEFSAADIQMSFPVEAAAQRAGLDASRPKLMAFLKRIHARSAYKRALERGGP
                     YSFAND"
     gene            2415..>3392
                     /locus_tag="VNU48_06835"
     CDS             2415..>3392
                     /locus_tag="VNU48_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWI11028.1"
                     /translation="MYAPFFGLKHEPFSIAPDPRYLFMSERHREALAHLLYGVNGGGG
                     FVLLSGEIGAGKTTVCRCFLEQIPRRTNVAYIFNPKLTVIELLQSVCEEFGIPPPQGT
                     RGEPTVKDYLDPLNEFLLKTHAVGQNNVLIIDEAQMLSADVLEQLRLLTNLETNERKL
                     LQIILIGQPELRDLLARPELEQLAQRVIARYHLGALTEDETERYIRHRLSVAGMTVAM
                     PFDAKAMQRVHQLSRGVPRRINLLCDRALLGAYSSGTGQVDRAIVDKAAAEVFGRGDG
                     GAPAIGLTGRRRAIGSRVALAAAVVAAVALAISLMGRNDAPPLAAASAPR"
BASE COUNT          473 a         1231 c         1174 g          514 t
ORIGIN      
        1 ggtgacgcgc tcgagatcgc gatcacgctc gagcccggcg cgcacgcact gctcaccacg
       61 cccggcgcga cccgcttcta ccgcagcacc ggggcggccg cgctgcagtc tctgcgcgcg
      121 acgctggcgc gcgacgcgcg cctcgaatgg ctgccgctgg agacgatcgc ctacagcggc
      181 tgcagcgccg cgaaccggat gcgcttcgag ctcgcgcccg gcgccgagat gatcggctgg
      241 gacctgctcg cgctcggcct gccggcatcc ggccagccct tcgtgcgcgg ccgattcacg
      301 cagacggtcg agctgccggg gcgctgggtc gagcacggcg tgatcgacgc atcggacgca
      361 tgcctgctcg attcgccgct cggctgggcc ggccggcggg tgctggcgac gctgtggttc
      421 gcggccggca gcgcgctggc gccgacgcga cgcgatgccc tgctcggcgc ggcgcgcgac
      481 gcggccgagg ccgacgcgct gcgcgccagc gccggcgcca ccgcggtgca cgatggcgtg
      541 gtcgtgctgc gcgcgctggc gccgcgcgtc gaaccggcga tggcgctgct gacgcgcacc
      601 tggtccgcct ggcgcgaaat cgcctggcaa ctgcccgcct gcgcgccgcg cgtctggcga
      661 acctgacccg gcgcgtgact tcctgcgcgc ccggcgcact ctgttaccgg cgccggcgca
      721 ccacggcgcc gccgggcttt ctagactttg cgcatgaaca tcaccccgcg cgcacttcac
      781 gccccacgtt ccctcgtgcg acccgctcac ctgctggtgg tcggaaccgc gctgctgctg
      841 tgtacccagg tgcacgcgat gcagcgctgc cgcatcgacg gccggctcgt ctaccaggcc
      901 tcgccctgcc cggaagcgac gcaggtcgcg ccgcgcagcg ccgagctgcc gcacacgctg
      961 gccgccgtcg atgccgacgt gccggtcaag cgcacgatgg ccgaggtgat gcgcgagcgc
     1021 gaagccgcga ttcgcgcgcg gccgaccgcg agcgattcgc gccctgacgg cgccaagatc
     1081 ctgcgcgacc ggatgggcgc gctctagacg cttcggccgt accggcagcg ttgctttccg
     1141 ttggttctat agtgccgtcg tggcgggacc atgacgatga agcacgcgat cttccttgcc
     1201 ggcgtcttgt ggctgtcgat ggcccaggcg gccgacatct accagtgggt cgacgagtcc
     1261 gggcgcaccc acctgtccga tgccgtgccc gagaagtaca agcacagtgc caagcgcgtc
     1321 gattcgcgct tgttcgaggt cagcccgcag cagcgcgccg aggcggaagc gcgcgccgcg
     1381 cgcgagcgcg aacaggcgat gaagggcgcc gctgatgcgc ctgtgggagc gcgtggcgtc
     1441 gcagcaccgg cgtcggccgc aacgccggcc agtgcgccga gcaaggcgtc cgactgtgcg
     1501 accttgcggc gtcagtatgc gcagagccag gagtgcttcg cgccgttcat gaacaccaac
     1561 ggctccatca agcctggcgc cttcgagaag tgcacgcagc ttcccgaccc gtcgcccaaa
     1621 tgcgggcctg aacccagccc ctgagccgtc aagcgcccgt cagtcgttcg cgaagctgta
     1681 cggcccgccg cgctccagcg cgcgcttgta ggccgagcgc gcgtggatgc gcttcaggaa
     1741 cgccatcagc ttcggccggc tcgcgtcgag cccggcgcgc tgcgcggcgg cctcgaccgg
     1801 gaagctcatc tggatgtcgg cggcgctgaa ctcgttgccg gcgaaccatt cgctattgcc
     1861 gagctcggcc tccatgaagt cgagctggcg cttcaggttc gggtcgacca tcgcggccag
     1921 cactttgttc gccaggccgc gcgcgatcgg cttggcgaag aacggcatcg gcgtcacctt
     1981 gatgcgctcg aagaccagct tcagcagcag cggcggcatc gccgagccct cggcgaagtg
     2041 cagccagtag gtccagcgca gccgctgcgg cgagcccgcc gcgggcacga ggcgaccgtt
     2101 gccgtaacgc tcgagcaggt actcgacgat cgcaccggtc tcggccaccg tcgtgtcgcc
     2161 atcggtgatc accggtgact tgcccagcgg gtgcaccttc atcagcgccg gcggcgcgag
     2221 catcgtctgc gggtcgcgct ggtacttctg gatctcgtat ggcaccccca gttcttcgag
     2281 cagccacagc acgcgctgcg atctggagtt gttcaggtgg tggacggtga tcatggctca
     2341 gctcgcggcg gttttcgggt gcccgggatt gtgaccgggt cgtcccgccg cgcgccgcgg
     2401 tattctccgc ccccatgtat gcgcctttct tcggtctgaa gcacgagccg ttctcgatcg
     2461 ctcccgaccc gcgctacctg ttcatgagcg agcgccaccg cgaggcgctg gcgcacctgc
     2521 tgtacggcgt caacggcggc ggcggcttcg tgctgctcag cggcgagatc ggtgccggca
     2581 agaccacggt gtgccgctgc ttcctcgagc agatcccgcg ccgcaccaac gtcgcctaca
     2641 tcttcaaccc caagctcacg gtgatcgagc tgctgcagtc ggtgtgcgag gagttcggca
     2701 tcccgccgcc gcagggcacg cgcggcgagc ccaccgtcaa ggactatctc gacccgctca
     2761 acgagttcct gctgaagacg cacgcggtcg gccagaacaa cgtgctgatc atcgacgagg
     2821 cgcagatgct gtcggcagac gtgctcgagc agctgcgcct gttgacgaat ctcgagacca
     2881 acgagcgcaa gctgctgcag atcatcctga tcggccaacc cgagctgcgc gacctgctcg
     2941 cgcggcccga gctcgagcag ctcgcgcagc gcgtgatcgc gcgctaccac ctgggcgcgc
     3001 tgaccgagga cgagaccgag cgctacatcc gacatcgcct gtcggtggcc ggcatgacgg
     3061 tggcgatgcc cttcgacgcc aaggcgatgc agcgggtgca ccagctctcg cgcggcgtgc
     3121 cacgccgcat caacctgctg tgcgaccgcg cgctgctcgg cgcctattcg agcggcaccg
     3181 gtcaggtcga ccgcgcgatc gtcgacaagg ccgccgccga ggtgttcggc cgtggcgacg
     3241 gtggcgcgcc ggccatcggc ctgacgggcc ggcggcgcgc gatcgggtcg cgggtcgcac
     3301 tggccgccgc ggtcgtggcc gccgtcgcgc tggcgatcag cctgatgggc cgcaacgacg
     3361 caccgccgtt ggccgccgcg tcggcaccgc gg
//
LOCUS       DATMJB010000313         2127 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_415121, whole genome shotgun sequence.
ACCESSION   DATMJB010000313 DATMJB010000000
VERSION     DATMJB010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415121"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1337)
                     /locus_tag="VNU48_06840"
     CDS             complement(117..1337)
                     /locus_tag="VNU48_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWI11029.1"
                     /translation="MATAAAAKNLKELVFEWEGKDKNGKVVRGEMRAGGEAVVGASLR
                     RQGILVNKVKKRRMNGGKAIKQKDIAIFTRQLTTMMRAGVPLIQAFDIVARGSTNPRM
                     TRLLTDIRSDVETGTSLSAAFRKHPLYFDALYCNLVEAGEAGGILEQLLDRLAIYQEK
                     TMAIKAKIKSALIYPIAVMVVAFIVLAVIMIFVIPAFKDVFSSFGADLPAPTLVVIAM
                     SEFFVHWWYLIFGILIGGTYFFIQSWRRSVKMQKFMDRLMLRVPVFGDLVRKSAVARW
                     TRTLSTMFAAGVPLVEALDSVGGASGNAVYAEATEQIQKDVSTGSALTTSMQTTGVFP
                     TMVVQMCAIGEESGSLDAMLGKAAEFYEDEIDEAVKGLSSLMEPFIIVILGVLVGGIV
                     VSMYLPIFKLGAVV"
     gene            complement(1411..>2127)
                     /locus_tag="VNU48_06845"
     CDS             complement(1411..>2127)
                     /locus_tag="VNU48_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWI11030.1"
                     /translation="SGKTVSLYTCLNILNQPGVNISTVEDPAEINLPGVNQVNVNDKA
                     GLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQTGHMVMSTLHTNDAPTTLTR
                     LLNMGVAPFNIASSVLLITAQRLARKLCETCKTQADYPREAMLKAGFADHDLDGTWRP
                     YRAVGCSACSNGYKGRVGIYQVMPVTEEIQRIILNEGTSMDISNQAQRENVRDLRQSG
                     LIKVRAGVTTLEEVISVTNE"
BASE COUNT          368 a          690 c          673 g          396 t
ORIGIN      
        1 cggcaggcgg tggatcacga cgttcaggaa gctgccgatg cacagcccca gcaaggccag
       61 gacgaacggc gacagcaagg cgcggacgac ttgcgggtcc atcgacgacg gtgtgatcag
      121 acgaccgcgc cgagcttgaa gatcggcagg tacatcgaca ccacgatgcc gccgaccagc
      181 acccccagga tcacgatgat gaacggctcc atcaggctgg acaggccctt gaccgcttcg
      241 tcgatctcgt cttcatagaa ttccgcggcc ttgccgagca tcgcgtcgag cgagcccgac
      301 tcctcgccga tcgcgcacat ctgcaccacc atcgtcggga acacgccggt ggtctgcatc
      361 gaggtggtca gcgccgagcc ggtggagacg tctttctgga tttgttcggt cgcttcggca
      421 tagaccgcgt tgcccgacgc gccaccgacc gaatcgagcg cctcgaccag cggcacgccg
      481 gcggcgaaca tcgtcgacag cgtgcgcgtc cagcgtgcaa ccgccgactt gcggaccagg
      541 tcgccgaaca ccggcacgcg cagcatcagg cggtccatga acttttgcat cttcaccgag
      601 cgccgccagg actggatgaa gaagtaggtg ccgccgatga ggatgccgaa gatcaggtac
      661 caccagtgga cgaagaactc cgacatcgcg atcacgacca gcgtcggcgc cggcaggtcg
      721 gcgccgaagg acgagaacac gtccttgaag gccggaatca cgaagatcat gatgaccgcg
      781 agcacgatga aggccacgac catcacggcg atcgggtaga tcagcgccga cttgatcttg
      841 gccttgatcg ccatcgtctt ttcctggtag atcgccaggc gatccagcag ctgctccaag
      901 atgccgccgg cctcgccggc ctcgaccagg ttgcagtaca gcgcgtcgaa gtacagcggg
      961 tgcttgcgga acgccgccga cagactggtg ccggtctcga cgtcgctgcg gatgtcggtc
     1021 agcagccgcg tcatgcgcgg gttggtgctg ccgcgcgcga cgatgtcgaa ggcctggatc
     1081 agcggcacgc cggcgcgcat catcgtcgtc agctggcgcg tgaagatggc gatgtctttc
     1141 tgcttgatcg ccttgccgcc gttcatgcgg cgcttcttga ccttgttgac caggatgccc
     1201 tggcgccgca ggctcgcgcc gaccacggct tcgccgccgg cacgcatctc gccgcgcacg
     1261 accttgccgt tcttgtcctt gccttcccac tcgaagacaa gctccttgag gttcttcgcg
     1321 gccgctgcag tcgccatggt cacctgcgag gaatgtcttc cccgcccctt cgatgtgttg
     1381 ctgtgttcgc tctggtccgt tcccgacggc tcattcgttc gtcaccgaga tcacctcttc
     1441 gagcgtcgtg acgccggcgc gcaccttgat cagccccgac tggcgcagat cgcgcacatt
     1501 ctcgcgctgg gcctggttcg agatgtccat cgaggtgcct tcgttcagga tgatgcgctg
     1561 gatctcttcg gtgaccggca tgacctgata gataccgacc cgccccttgt agccgttgct
     1621 gcaggccgag caaccgaccg cgcgatacgg gcgccaggtg ccgtcgaggt cgtggtcggc
     1681 gaagccggcc ttcagcatcg cctcgcgagg gtaatcggcc tgcgtcttgc acgtctcgca
     1741 aagtttgcgc gccagacgct gcgccgtgat caacagcaca ctggacgcaa tattgaaagg
     1801 cgcgacgccc atgttcagca gccgcgtcag ggtcgtcggc gcatcgttgg tgtgcagcgt
     1861 cgacatcacc atgtgaccgg tctgggccgc cttgatcgcg atgtcggcgg tttccaggtc
     1921 gcggatttcg ccgaccatga tgatgtcggg gtcctgccgc agaaaggact tcagcgccgc
     1981 ggcgaaagtc agcccggcct tgtcgttgac gttgacctgg ttgacgccgg gcaggttgat
     2041 ttcggccggg tcttcgacgg tcgagatgtt gacgcccggc tggttcagga tgttcaggca
     2101 ggtgtagagc gacaccgtct tgcccga
//
LOCUS       DATMJB010000314         2036 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_17765, whole genome shotgun sequence.
ACCESSION   DATMJB010000314 DATMJB010000000
VERSION     DATMJB010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17765"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..476
                     /locus_tag="VNU48_06850"
     CDS             30..476
                     /locus_tag="VNU48_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWI11031.1"
                     /translation="MFAKRKKQPTIELSKLSSLVAEDVEIVGDLSFAGGIRIDGRIKG
                     NVIARAVDGQPRALLVLSEKGRIEGRVNCGDAVINGSVVGDLEVENFLELQSNARVSG
                     TIRYQHLRMDVGAEVRGQLLKPDDALPGTLNVVELGADKAAGARLR"
     gene            complement(500..1234)
                     /locus_tag="VNU48_06855"
     CDS             complement(500..1234)
                     /locus_tag="VNU48_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11032.1"
                     /translation="MNMHNTSRRGALMALTLVLAPFAAMAQPRERDEGRFRILQARYG
                     TARHNVDVTRRLQELARADQQIRISNQTFGVDPDQGVGKTVRIFARTPDGRTRTFEYA
                     EGDVIDGARFVGWGGGRWGRSGYRGGWGRDAERGGGGSDSGEYRIVQALYGTKEHNVD
                     VTPRLRELARSDQTFRLGNDTFGVDPDRGRGKTLRILARGPDGQTRMFEYREGSAVDG
                     SRFTGWSRGDWGQDGQRAGWGDARPR"
     gene            1499..1657
                     /locus_tag="VNU48_06860"
     CDS             1499..1657
                     /locus_tag="VNU48_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11033.1"
                     /translation="MRLDRPQGPGARRTILDGLILRFGSQALDEAERGQLNRVWHRLD
                     PGPTRSRG"
     gene            1678..1929
                     /locus_tag="VNU48_06865"
     CDS             1678..1929
                     /locus_tag="VNU48_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11034.1"
                     /translation="MISTLSNGNIALLGIERHELLMVAAHPSDLRGAQRAGSPTALVR
                     RPLEYGPNPAGAPPPDALPDDRFDLVASDFVDLARCLGA"
BASE COUNT          287 a          744 c          719 g          286 t
ORIGIN      
        1 gcaccctgca ccgacaccgg agaccgacca tgttcgcgaa acgcaagaag caaccgacca
       61 tcgagctgag caagctctcc agcctggtgg ccgaggacgt cgagatcgtc ggcgacctga
      121 gcttcgccgg cggcatccgc atcgacgggc gcatcaaggg caacgtgatc gcgcgtgccg
      181 tcgacggcca gccgcgcgcg ctgctggtgc tgtccgagaa ggggcgcatc gagggccgcg
      241 tgaactgcgg cgacgcggtg atcaacggca gcgtcgtcgg cgacctcgag gtcgagaatt
      301 tcctcgagct gcaatcgaac gcccgcgtca gcggcacgat ccgctaccag cacctgcgca
      361 tggacgtggg cgccgaggtc cgcgggcagc tgctgaagcc cgacgatgcg ctgcccggca
      421 cgctcaacgt cgtcgagctg ggcgcggaca aggccgccgg cgcgcggctg cgctgagaag
      481 agcctgaccc ggcgcgcgct cagcgcgggc gcgcgtcgcc ccagccggcg cgttgaccgt
      541 cctggcccca gtcgccgcgg ctccagccgg tgaagcggct gccgtcgacg gcgctgccct
      601 cgcggtactc gaacatccgg gtctggccgt ccgggccgcg cgccaggatg cgcagcgtct
      661 tgccgcgccc ccggtccggg tcgaccccga aggtgtcgtt gccgaggcgg aaggtctggt
      721 cgctgcgcgc gagttcgcgc aggcgcggcg tcacgtcgac gttgtgctcc ttggtgccgt
      781 acagcgcctg cacgatccgg tactcgccgc tgtcgctgcc gccaccacct cgttcggcgt
      841 cacggcccca gccgccgcgg tagccggacc ggccccagcg gccgccgccc cagccgacga
      901 agcgtgcgcc gtcgatgaca tcgccctcgg cgtattcgaa ggtgcgcgtg cggccgtcgg
      961 gggtgcgcgc gaagatgcgc accgtcttgc cgacaccctg gtccgggtcg acgccgaagg
     1021 tctggttgct gatgcggatc tgctgatcgg cgcgtgccag ttcctgcagg cgccgggtca
     1081 cgtccacgtt gtgccgcgcc gtgccgtagc gcgcctgcag gatccggaac cggccctcgt
     1141 cgcgctcgcg cggctgcgcc atggcagcga agggcgccag gaccagggtc agcgccatca
     1201 gcgccccgcg tctcgacgtg ttgtgcatgt tcatgtctcg ctcctcgtga ctggtcgatc
     1261 gtcacgcgcc gcgccgccgt ggcgttgcgc gctctccggt gcatcgaaag ccggcgcacc
     1321 tgtgccaacc atcgtgggcg cacgaggcgc agcgcggcgt cggacaacgc cgcgacaaag
     1381 gtattctcgg aaccccgaga tcgtgaccga caggaggaag cgacgatgag cgaattcgat
     1441 cagggcgcga tccgcgccct ggtgttcgac gtgttcggcc ggcaccagtc gtcgactggt
     1501 gcgcctcgat cgtccgcaag ggccaggcgc tcgccgcacg atcctcgacg gcctgatcct
     1561 gcgcttcggc tcgcaggcgc tggacgaggc cgagcgcggg cagctgaacc gcgtctggca
     1621 ccgcctcgac cctggcccga cgcggtcgcg gggctgacgc ggctgaagtc tcgcttcgtg
     1681 atctcgacgc tgtcgaacgg caacatcgcg ctgctcggca tcgagcgcca cgagctgctg
     1741 atggtcgccg cgcaccccag cgacctgcgc ggcgcacagc gcgccgggtc gccgaccgcg
     1801 ctggtgcgcc ggccgctcga atacgggccg aacccggccg gcgcgccacc gcccgacgcg
     1861 ctgccggacg accgcttcga cctcgtcgcg agcgacttcg tcgacctcgc gcggtgtctc
     1921 ggcgcgtgag cgaatcgcgc cgccgatgaa cgaagacaac gcgcgcgcgg tcacgctgct
     1981 gcaggccttc gagaccgcgc agcccgcccc ggcgaactgg ggcgacgacg accgtg
//
LOCUS       DATMJB010000315         4009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_611538, whole genome shotgun sequence.
ACCESSION   DATMJB010000315 DATMJB010000000
VERSION     DATMJB010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611538"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /gene="cysD"
                     /locus_tag="VNU48_06870"
     CDS             complement(<1..566)
                     /gene="cysD"
                     /locus_tag="VNU48_06870"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HWI11035.1"
                     /translation="MSTLTHLQRLEAESIQIMREVVAEADKPVMLYSIGKDSAVMLHL
                     ARKAFYPAPPPFPLLHVDTTWKFRDMYAMRERMARQMEMELIVYQNPEAKEMGINPFE
                     HGSQVHTDMWKTQGLKQALDKYGFDAAFGGARRDEEKSRAKERIFSFRTASHGWDPKT
                     QRPELWNLYNGRIAKGESIRAFPLSNWT"
     gene            795..2063
                     /locus_tag="VNU48_06875"
     CDS             795..2063
                     /locus_tag="VNU48_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI11036.1"
                     /translation="MPSRLALTRPGSTAIRIANQSDATRINDFIWMSEGTTNTYLVTT
                     AQGNVLVNTGLGIEAPIHKQCYDKVSDAPLRAIVLTQGHYDLVGGVELFKQADTRVIG
                     HRNMQACQDDDQRLQGFRNRRNMRFFPDFVAPMLEAQTRAQSLGYTPPQARARPDVSF
                     DERHHFELGGVRFELIWVPGGETTDSILVWLPDQKILFCGNTLGPLFPHMPNLHTIRG
                     DRLRFALPYIAACDTMLALEPELLLTGHFDPVVGKDLIRDELTRLRDAVRYVHDETVR
                     GMNEGRDPFTLMKAIRLPEHLQVGEAYGTVAWAVRAIWHGYGGWFYFQSTTEIGDTPV
                     REVYAELAEAAGCDALAERAAARLAADEPLHAIHLAEIALAGAPTHRPALQVYLDAHE
                     RMLAQSHPGNRWEHFWLQGEIDATRAKLAS"
     gene            2070..3323
                     /locus_tag="VNU48_06880"
     CDS             2070..3323
                     /locus_tag="VNU48_06880"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWI11037.1"
                     /translation="MTSLHLTDLAQPLLSEHQRRAMRDAERCPVTLSEQAVLQAAIDA
                     TGLDDFGEPDFRERLAVWLEAGTQDIGLNALGRAVLFGYCVRYASNRLRLEDLVRRHP
                     EILAERIARPVFIAGLPRSGTTSLINLMGRDARWRSLPYWEAVAPFPGLAGGFDSSLA
                     DPRRLQCLREWEQFDALLPHLKAMHEMSPDHIHEDLELQALDFTSYNIEWTAHVPRWR
                     DWYLAHDRQTHYAYLRKALQALQWQDRRAGRAPRRWLLKCPQHLENLGAVMQTFPDAT
                     LVLTHRDPLEVIQSAATMVAYGDRVRRHRIDLRLTTDYWIDRIARMLEACVRDRASVP
                     DAQSMDVPFAHYVADEAGTLERIYALAGEALNPDGARAVRNEIKARSDAHVRVAYDLF
                     DTLGIDTTALRQRFAFYLDRFPALR"
     gene            complement(3356..>4009)
                     /locus_tag="VNU48_06885"
     CDS             complement(3356..>4009)
                     /locus_tag="VNU48_06885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI11038.1"
                     /translation="LVIVASWGVAPSASRVAQRTDPPAPVPWRRLWPVYLLCVPLYAA
                     VFMTTTQVPFLLHADGVTSPSVQSGVLAMAAMWNAIGAALYGRVRDRIGGQQTFTTSL
                     ALMAIGQAVLGLSHHPLPMALGCALAGSGAGLAVPHVPAMVLARVQGVARGRALGLMY
                     SSLFFGSFCSPLFIAPVAAVLGRHGALLTSATLLAAGALAALLLARKSGDAGATSEN"
BASE COUNT          694 a         1403 c         1280 g          632 t
ORIGIN      
        1 tcggtccaat tggacagcgg gaaggcgcgg atcgactcgc ccttggcaat gcggccgtta
       61 tagaggttcc agagctcggg ccgctgggtc ttcggatccc agccgtggct tgccgtgcgg
      121 aacgagaaga tccgctcctt cgcccgcgac ttctcctcgt cgcgccgggc accgccgaaa
      181 gcggcgtcga acccgtactt gtcgagcgcc tgcttcaggc cctgcgtctt ccacatgtcg
      241 gtgtgaacct gcgacccatg ctcgaagggg ttgatgccca tctccttcgc ctcggggttc
      301 tgatagacga tgagttccat ctccatctgg cgggccatgc gctcgcgcat cgcgtacatg
      361 tcgcggaact tccaggtcgt gtccacgtgc agcagcggaa acggcggcgg cgcgggatag
      421 aacgccttgc gcgccaggtg cagcatcacg gcgctgtcct tgccgatcga gtagagcatc
      481 accggcttgt cggcttcggc gacgacttct cgcatgatct ggatgctctc ggcctcgagt
      541 cgctgcaggt gggtcaaggt gctcatgcgg ggcgtctcgc tttcatcttg gagtctgtcg
      601 gactggcggc gaatcggttc gatgggtcgc cgcagttcag gcctgaaggg taggcacaga
      661 cgcccttcaa ttcatcgtcc gaccggacta ccgggctcga tgcaagaagc tcggccagca
      721 ccttccgcta gtccaaccgg acgacgagac ggtccgtgcg agcccgcaca ctgcgtgcca
      781 cacgaggacc aacgatgccc agccgactcg ccctgacccg ccccggctcc accgccatcc
      841 gtatcgcgaa tcaaagcgac gcgacacgca tcaacgactt catctggatg tccgagggaa
      901 ccacgaacac ctacctcgtc accacggcgc aaggcaacgt gctcgtcaac accgggctgg
      961 gcatcgaagc cccgatccac aagcagtgct acgacaaggt gagcgacgcg cccttgcgcg
     1021 cgatcgtgct gacccagggc cactacgacc tcgtcggtgg cgtggagctg ttcaagcagg
     1081 ccgacacccg cgtcatcggg catcgcaaca tgcaggcctg ccaggacgac gaccagcgac
     1141 tccaggggtt ccggaaccgg cgcaacatgc gcttctttcc ggatttcgtc gcgccgatgc
     1201 tggaagcgca gacgcgcgcg cagtccctcg ggtacacgcc gccgcaggcc cgcgcccgac
     1261 cggacgtgag cttcgacgaa cgccaccatt tcgagcttgg cggcgtgcgc ttcgagctga
     1321 tctgggtgcc gggcggcgag acgaccgact ccatcctcgt ctggctgccg gaccagaaga
     1381 tcctgttctg cggcaacacg ctggggccgc tgtttcccca catgccgaac cttcacacga
     1441 ttcgcggcga ccgcctgcgc ttcgcgctgc cctacatcgc cgcgtgcgac acgatgctgg
     1501 cgctcgagcc ggaactgctg ctcaccggcc acttcgatcc cgtcgtcggc aaggacctga
     1561 tccgcgacga gctgacgcgg cttcgcgacg cggtccggta cgtccacgac gagaccgtcc
     1621 gcgggatgaa cgaaggccgc gatccgttca cgttgatgaa ggcgatccgc ttgcccgagc
     1681 acctgcaggt cggcgaggcc tacggcacgg tcgcctgggc ggtccgcgcc atctggcacg
     1741 gctatggcgg ctggttctac ttccagtcga ccacggagat cggcgacacg ccggtgcgcg
     1801 aggtctacgc cgaactcgcc gaagccgccg gctgcgatgc gctggctgag cgcgcggcgg
     1861 cgcgtctggc cgcggacgag ccgctgcatg ccatccacct ggcggagatc gctctggccg
     1921 gcgcccccac ccaccgacct gcgttgcagg tctatctcga tgcgcacgaa cgcatgctcg
     1981 cgcaaagcca ccccggcaac cgctgggagc acttctggct gcagggcgag atcgacgcca
     2041 cgcgggcgaa gctcgcgagc tgatcccgca tgaccagcct gcatctcacg gacctggcgc
     2101 agccgctgtt gagtgaacac cagcgccgcg ccatgcgcga cgcggaaaga tgcccggtga
     2161 ccttgtcgga gcaagccgtc ctgcaggcgg cgatcgacgc gaccggcctg gatgatttcg
     2221 gcgaaccgga ctttcgcgag cgcctggccg tgtggctgga agccggcacc caggacatcg
     2281 gcctcaatgc cctgggccgc gcggtcctgt tcggttactg cgtgcgctac gcatcgaatc
     2341 gcttgcgcct cgaggacctc gtccggcgtc accccgagat tcttgccgag cgcatcgcgc
     2401 ggccggtgtt catcgccggt ctgccgcggt ccggaacgac cagcctcatc aacctcatgg
     2461 gccgcgacgc gcgctggcgg tcgctgccgt actgggaggc ggtggcgccc ttcccgggcc
     2521 tggccggcgg gttcgattca agcctcgccg acccgcggcg cttgcagtgc ctgcgcgagt
     2581 gggagcagtt cgacgcgctg ctgccgcacc tgaaggcaat gcacgagatg tcgcccgacc
     2641 acatccacga agatctcgaa ctgcaggcgc tcgacttcac ctcgtacaac atcgaatgga
     2701 ccgcgcacgt gccgcgctgg cgcgactggt atctggcgca cgaccggcag acgcactacg
     2761 cgtaccttcg caaggcgctg caggccttgc agtggcagga ccgtcgggca gggcgagcgc
     2821 cgcgtcgctg gctgctgaag tgcccgcagc acctggagaa cctcggggcg gtgatgcaga
     2881 cctttcccga cgccacactg gtcttgacgc atcgggaccc gctggaagtg atccagtcgg
     2941 cagcgaccat ggtggcgtac ggcgatcgcg tcaggcgcca ccgcatcgat ctgcgcctca
     3001 ccaccgacta ctggatcgac cgcatcgcca ggatgttgga ggcctgcgtt cgcgaccgcg
     3061 cctcggttcc agacgcgcaa agcatggatg tgcccttcgc gcactacgta gccgacgaag
     3121 cgggcaccct ggaaagaatc tacgccctgg ccggagaagc gctgaacccc gatggcgcgc
     3181 gcgccgttcg aaacgagatc aaggcccggt ccgacgctca cgtccgcgtg gcctacgacc
     3241 tgttcgacac gcttggtatt gacacgactg ccttgcggca gcgattcgcc ttctacctcg
     3301 atcgctttcc ggcgctgcgg tgaatcagaa acgtccgggc cgggccttcg tcatttcaat
     3361 tctctgatgt ggccccggcg tctccggatt tccgagccag cagcaacgcc gccagcgcac
     3421 cggcggccag caaggtcgcg gatgtcagaa gcgcgccatg gcgtccgagc accgcagcga
     3481 ccggcgcgat gaacaacgga ctgcagaagc tgccgaaaaa caaggacgag tacatcaggc
     3541 cgagtgcgcg cccgcgcgcc accccttgca cgcgcgccag caccatggcg ggaacgtgcg
     3601 gaaccgccag ccccgcgcca ctgcccgcca gcgcgcagcc cagggccatt ggaagcggat
     3661 ggtgggaaag ccccaacacc gcttgcccga tcgccatcag ggccagactc gtcgtgaagg
     3721 tttgctgccc cccgatccgg tcacgcacac ggccatagag tgccgcgccg atggcattcc
     3781 acatcgcggc catcgccagc accccggatt gcaccgaagg gctcgtcacg ccgtcggcat
     3841 gcagcagaaa cggcacctgc gtggtggtca tgaagacggc cgcgtacagc ggcacacaca
     3901 gcagatacac gggccacagc cggcgccatg gcaccggggc cggcgggtcg gtgcgctgcg
     3961 cgaccctgga agcggagggc gcaacacccc agctggcaac gatcaccaa
//
LOCUS       DATMJB010000316         2815 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_328615, whole genome shotgun sequence.
ACCESSION   DATMJB010000316 DATMJB010000000
VERSION     DATMJB010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328615"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..428
                     /locus_tag="VNU48_06890"
     CDS             162..428
                     /locus_tag="VNU48_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HWI11039.1"
                     /translation="MSVAAADIEAGLRAALNPLSLEVQDDSHLHAGHAGAREGRHFTV
                     RITAERFNGLSRVARHRLIYDALQLLIPRGIHALAIEARAPGES"
     gene            456..1274
                     /locus_tag="VNU48_06895"
     CDS             456..1274
                     /locus_tag="VNU48_06895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWI11040.1"
                     /translation="MKMNLFAAGAALLLSATLALPLAAQAQNIAIVNGKPVPKARADA
                     LIKQVQSQATAQNQQLPPDLEQRVRDKLVMDEIFTQEAEKRGMAATPEYKSQMEQARQ
                     AVLTQALLADFVKTHPVSDDEVKAEYEKFKAQSAGTEYRARHILVEKEDEAKALIKQI
                     NGGANFEELAKKNSKDPGSGANGGDLDFAAPGSYVPEFSQAMVKLKKGEMTQEPVKTQ
                     FGFHIIKLEDTREAQFPKLDDVKGQLKQRMEQQKLAAYRDEIRAKAKTDYKFTN"
     gene            complement(1336..2037)
                     /locus_tag="VNU48_06900"
     CDS             complement(1336..2037)
                     /locus_tag="VNU48_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI11041.1"
                     /translation="MSEPIIAVERVTKRVHDSTGTLTILHEIDFSLAPRQSAAIVGAS
                     GSGKSTLLAIIAGLDTPTSGTVRLAGTDLFTIGEDARAAVRAAKLGFVFQSFQLLGNL
                     NALENVMLPLELQGRSDARVLATEMLRRVGLGERLRHYPKVLSGGEQQRVALARAFVV
                     RPAVLLADEPTGSLDFATGATVMELMFALNREAGTTLVLVTHDASIAARCDRQLRIEA
                     GRVQADEGVAERQAQ"
     gene            2024..2728
                     /locus_tag="VNU48_06905"
     CDS             2024..2728
                     /locus_tag="VNU48_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HWI11042.1"
                     /translation="MGSDMNDEEVPGSRARRRWLGHCSVLGVAAVLAWPATATAQGPR
                     PAAAPLILVVGDSLSAEYGLPRGSGWVALLQARLAQQKIAADVVNASISGDTTSGGRT
                     RLPTLLKQQRPAIVVLELGANDALRGLPLAMTYDNLDAMTQAAQAAGAKVVMVGMQLP
                     PNYGRTYGEEFAALFARLAKARGTALVPFLLKGVADVPQAETLFQRDGIHPIAAAHPT
                     ILANVWPVLQPLLSKR"
BASE COUNT          488 a          962 c          961 g          404 t
ORIGIN      
        1 ccgggggaca tgagcgcagc agcacgcccc accggtccga tcccgcgcca aaccgtgcac
       61 ggcggacatt caccgacagc atcgggccga aagcacacac ccagattcat gccgacgttt
      121 catgcgtcgc gggtcgcgcg acgcgcggtg gagcaagtga gatgagcgtt gctgccgcag
      181 acatcgaggc gggcctgcgc gccgcgctga accccctgtc gctcgaggtg caggacgaca
      241 gccacctgca tgccggtcat gccggcgcgc gcgagggccg ccatttcacg gtgcggatca
      301 ccgccgaacg cttcaacggt ttgtcacgag tggcgcgcca ccgtctcata tatgatgcgc
      361 tgcaactttt gatcccgcgc ggcatccacg ccttggccat cgaggcgcgc gcgcccggtg
      421 aatcctgagt tgaaccccca acttccacca tgctcatgaa gatgaatctt ttcgccgcgg
      481 gcgcggcgct gctgctgtcc gcaacgctgg cgctgccgct ggccgcccag gcgcagaaca
      541 ttgcgatcgt caacggcaag ccggtgccca aggcgcgcgc cgacgcgctg atcaagcagg
      601 tgcagtcgca ggccactgcg cagaaccagc agttgccgcc cgacctcgag cagcgcgtgc
      661 gcgacaagct cgtgatggac gaaatcttca cgcaagaggc cgagaagcgc ggcatggccg
      721 ccacgcctga atacaagtcg cagatggagc aggcgcgcca ggcggtgctg acgcaggcgc
      781 tgctcgccga cttcgtcaag acgcacccgg tcagcgacga cgaggtcaag gccgagtacg
      841 agaagttcaa ggcccagtcg gccggcaccg aataccgcgc gcgccacatc ctggtcgaga
      901 aggaagacga ggccaaggcg ctgatcaagc agatcaacgg cggcgcgaac ttcgaggagc
      961 tggcgaagaa gaactcgaag gacccgggat cgggcgccaa cggcggcgac ctcgatttcg
     1021 cggcgccggg ctcgtacgtg cccgagttct cgcaggccat ggtcaagctg aagaagggcg
     1081 agatgacgca ggagccggtc aagacccagt tcggcttcca catcatcaag ctcgaggaca
     1141 cgcgcgaagc gcagttcccg aagctcgacg acgtgaaggg tcagctcaag cagcgcatgg
     1201 agcagcagaa gctcgcggcc tatcgcgacg agatccgcgc gaaggcgaag acggactaca
     1261 aattcacgaa ctgaacgctt cgagaattga acgcttcgct actgtgacaa gggcgccgct
     1321 ggcgcccttg ttccgttatt gggcttgcct ttcggccacg ccttcgtcgg cctgcactcg
     1381 gccggcctcg atgcgcaact gccgatcgca gcgcgccgcg atcgacgcgt cgtgcgtgac
     1441 cagcaccagc gtcgtgccgg cctcgcggtt cagcgcgaac atcagttcca tcacggtcgc
     1501 gccggtcgcg aagtcgaggc tgccggtggg ctcgtcggcg agcaacacgg ccggccgcac
     1561 gacgaaggcg cgcgccagcg cgacgcgctg ctgctcaccg cccgacaaga ccttcgggta
     1621 gtggcgcagg cgttcgccca ggccgacgcg gcgcagcatc tcggtggcga gcacgcgcgc
     1681 atcgctgcgc ccctgcagtt ccagcggcag catcacgttc tcgagcgcgt tcaggttgcc
     1741 cagcaactgg aaactctgga acacgaaccc cagcttggcg gcgcgtaccg cggcgcgcgc
     1801 gtcttcgccg atcgtgaaca ggtcggtacc cgcgaggcgc acggtgccgc tggtcggcgt
     1861 gtccaggccg gcgatgatcg ccagcagcgt gctcttgccc gagcccgagg ctccgacgat
     1921 cgccgccgac tggcgcggcg ccagcgaaaa atcgatctca tggagaatgg tcagcgtacc
     1981 ggtggagtcg tgtacccgct tggtgacgcg ctcgaccgcg atgatgggtt cagacatgaa
     2041 tgatgaagag gttcctggtt cgcgcgcgcg gcgccgttgg ctggggcact gtagcgtgct
     2101 tggggtcgcg gccgtgttgg cgtggccggc gaccgcaacg gcccaggggc cgcgcccggc
     2161 ggccgcgccg ctgatcctgg tcgtcggcga cagcctgtcg gccgagtacg gcctgccgcg
     2221 cggcagcggc tgggtcgcgc tgctgcaggc ccggctggcg cagcagaaga tcgcggccga
     2281 cgtcgtcaac gccagcatca gcggcgacac gacctcgggc ggccgcacgc gcctgccgac
     2341 gctgctgaag cagcagcgcc ccgccatcgt cgtgctcgaa ctcggcgcga acgatgcgct
     2401 gcgcggcctg ccgctggcga tgacctacga caacctcgat gcgatgaccc aggcggcgca
     2461 agcggcaggc gccaaggtcg tgatggtggg catgcagctg ccgccgaact acggtcgcac
     2521 gtacggcgag gagttcgcag cactgttcgc gaggcttgcg aaggcccgtg gcaccgcgct
     2581 cgtgcccttt ctgctgaagg gcgtggccga cgtgccgcag gccgagacgc tgttccagcg
     2641 cgacggcatt cacccgatcg ccgccgcgca cccgacgatc ctggccaacg tctggccggt
     2701 gctgcagccc ctgctgtcga agcgctgaag gcgcgcgccg aaccctgcac tagcgctgcg
     2761 ggtcgccgcg cttcggctct tcgccgcgcg ggccctcggg gcgccgcggc tcggc
//
LOCUS       DATMJB010000317         3374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_297494, whole genome shotgun sequence.
ACCESSION   DATMJB010000317 DATMJB010000000
VERSION     DATMJB010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297494"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2083
                     /locus_tag="VNU48_06910"
     CDS             <1..2083
                     /locus_tag="VNU48_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HWI11043.1"
                     /translation="ALLMPARDPRAELLGITTVFGNAAVERTTANALALRERFGIEAP
                     VARGAARPLVRAPAGYAGLHRGRCRARAGRVPRLLYALTGYRGVRAFRMPTFRQPMAV
                     PESMTLVRGSRHLLLAVLLAALCAAAHAARPVALRLIGINDFHGNLEPANLSLMLADP
                     QAPQGTPLRVPAGGAAAVAGLVHTLRGGAPHSLFLSAGDLIGAAPLVSTMFRHESTIA
                     VMNAIGLDVGAVGNHEFDAGIAELQRVARGGCAANLPDDAVSSCGLERYGGAKFPMLA
                     ANVLDARGRPVLAPYVVRRFAGIRVGIIGAVTKTTPSIVVPSGVAGLRFTDEADAVNR
                     AARALKAQGVKAIIAVFHEGGELGTPQQRGDWNDTRCPGRSGPIFAIAQRLVPEISVI
                     FTAHTHQGYRCIIDGRTIIQGTSYGRGVSVVDVALDPATREILPAKTRSINLPVVNDS
                     TDAATRERLAAALPAPYAQVLRATRPDPAIAAQVARYAAIVAPKAERIVGRIGGRFAR
                     GGPVDSAAGRLIADAQLAATREPAQGGAQIAFMNPGGIRSDLDCNGTPPCAVSFGQVF
                     TMQPFGNSLVVMTLTGAQIKTLLESQQKPHDMTVLQPSEGFTYTWQADAPAGERVRDM
                     RLGGETLVPERDYRVTVNSFMAEGGDGFVTLTRGTARSGGGQDLEALLAYLKGPAERS
                     PVPAPRITRLP"
     gene            2153..3286
                     /locus_tag="VNU48_06915"
     CDS             2153..3286
                     /locus_tag="VNU48_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWI11044.1"
                     /translation="MSLAPLFLVSLVLHAYVGARVAPALPDGAAWFVALLAASALLMP
                     MTLLARRVRSQPWADRLAWAGALAMGLFSSLFVLTLLRDALLLVAALIGWIAPEALAG
                     LPLTRWSAVAVPLLGVIVSVWGFVNARRTAAVVSVDVPITGLPAGLHGFTIVQISDIH
                     VGPTIKRAYLHRIVESVNRLGADMVAVTGDLVDGSVRELGSHVAPLAGLSSRHGTFFV
                     TGNHEYYSGAPAWVAELRRIGLRVLMNEHVVLEHAGAALLVGGVTDFHAHHYDPAQRS
                     DPQAALAGAPTHASTRVLLAHQPRSAEAAERAGFQLQLSGHTHGGQFLPWNLFVRLQQ
                     PFTAGLHRLRRMWVYTSRGTGYWGPPKRFGAPSEITRLRLVSV"
BASE COUNT          419 a         1230 c         1224 g          501 t
ORIGIN      
        1 ggcgctgctg atgccggcgc gcgacccgcg cgccgagctg ctgggcatca cgaccgtgtt
       61 cggcaacgca gcggtcgagc gtacgaccgc gaacgcgctg gcgctgcgcg agcgcttcgg
      121 catcgaggcg ccggtggcgc gcggcgcggc gcgacctctc gtgcgagcgc cggcgggcta
      181 tgccggtctg catcgcggtc gatgccgagc gcgtgcgggc cgagttccgc gcctgcttta
      241 tgcgctgacg ggctatcgtg gcgttcgtgc ctttcgcatg ccgacttttc gacaacccat
      301 ggccgtgccc gaatcgatga cgctggtacg tggcagccgc cacctgcttc tggcggtgct
      361 gctggcggcc ctgtgcgccg ctgcacacgc cgcccggccg gtggcgctgc gcctgatcgg
      421 catcaacgac tttcacggca acctcgagcc ggcgaacctg agcctgatgc tggccgaccc
      481 gcaggcgccg cagggcacgc cgctgcgtgt gccggcgggt ggtgccgcgg cggtggccgg
      541 gctcgtgcac acgctgcgtg gcggtgcgcc gcacagcctg ttcctgtcgg ccggtgacct
      601 gatcggcgcc gcgccgctgg tgtcgacgat gttccggcac gagtcgacga tcgcggtgat
      661 gaacgcgatc ggcctggacg tcggcgctgt cggcaaccac gagttcgatg ccggcatcgc
      721 cgagctccag cgcgtcgcgc gcggcggctg tgcagcgaac ttgcccgacg atgccgtgag
      781 ctcctgcggc ctggagcgct acggcggcgc gaaattcccg atgctggccg ccaacgtgct
      841 cgacgcgcgg ggccggccgg tgctcgcgcc ctacgtggtg cggcgcttcg ccggcatccg
      901 cgtcggcatc atcggcgcgg tgacgaagac gacgccgagc atcgtcgtgc cctcgggcgt
      961 ggccgggctg cgcttcaccg acgaggcgga cgcggtgaac cgcgcggcgc gcgcgttgaa
     1021 ggcgcagggc gtgaaggcga tcatcgcggt gttccacgag ggcggcgaac tcggcacccc
     1081 gcaacagcgc ggcgactgga acgacacccg ctgcccgggc cgctccggcc cgatcttcgc
     1141 catcgcgcag cgcctggtgc ccgagatcag cgtgatcttc acggcccaca cgcaccaggg
     1201 ctaccgctgc atcatcgacg gccgcacgat catccagggc acctcgtacg gacgcggcgt
     1261 ctcggtggtc gacgtcgcgc tcgatccggc gacgcgcgag atcctgcccg cgaagacccg
     1321 cagcatcaac ctgccggtcg tcaacgacag caccgatgcg gcgacgcgcg agcgcctcgc
     1381 cgcggcgctg ccggcgccgt atgcgcaggt gctgcgcgcg acccggcccg atccggcgat
     1441 cgccgcgcag gtggcgcgct acgcagcgat cgtggcgccg aaggccgaac gcatcgtcgg
     1501 ccgcatcggc gggcgctttg cccgcggcgg gccggtcgac agcgccgccg ggcggctgat
     1561 cgccgacgcc cagctcgcgg ccacgcgcga gccggcgcag ggcggggcgc agatcgcgtt
     1621 catgaacccg ggaggcatcc gcagcgacct cgactgcaat ggcacgccgc cctgcgcggt
     1681 gagcttcggc caggtgttca cgatgcaacc cttcggcaac agcctggtcg tgatgaccct
     1741 gaccggcgcg cagatcaaga cgctgctcga gtcgcagcag aagccgcacg acatgacggt
     1801 gctgcagccg tccgagggct tcacctacac ctggcaggca gacgcgccgg ccggcgaacg
     1861 cgtgcgcgac atgcgcctgg gcggcgagac cctcgtgccg gagcgcgact accgcgtcac
     1921 ggtcaacagc ttcatggccg agggcggcga cggcttcgtc acgctgaccc gcggcacggc
     1981 gcgcagcggc ggcggccagg acctggaggc gctgctggcc tacctgaagg gcccggccga
     2041 gcgttcgccg gtgccggcgc cgcgcatcac ccggctgcca tagcgcccgt cgcccttgcg
     2101 cgcgggcgtt tcgcaaccac atgtggcgga ctgactgctg ccgctggttc cgatgtcgct
     2161 tgctccgctg ttcctggtct ccctcgttct gcatgcctac gtcggcgcgc gtgtcgcgcc
     2221 ggcattgccg gacggcgccg cgtggttcgt cgcgctgctc gcggcctcgg cgctgctgat
     2281 gccgatgacg ctgctcgcgc ggcgcgtgcg cagccagccc tgggccgacc ggctcgcctg
     2341 ggccggtgcg ctggcgatgg ggctgttctc gtcgctgttc gtgctgacgc tgctgcgcga
     2401 tgcgctgctg ctcgtcgccg cgctgatcgg atggatcgcg cccgaggccc tggccggcct
     2461 gccgctgacg cgctggagcg cggtggccgt gccgctgctc ggcgtgatcg tcagcgtctg
     2521 gggcttcgtc aacgcgcggc gcaccgcggc ggtggtgtcg gtcgacgtgc cgatcaccgg
     2581 tctgccggcc gggttgcacg gcttcacgat cgtgcagatc agcgacatcc atgtcggccc
     2641 gacgatcaag cgcgcgtacc tgcaccgcat cgtcgagtcg gtgaaccggc tcggcgccga
     2701 catggtggcg gtgaccggcg acctcgtcga cggctcggtg cgcgagctcg gcagccacgt
     2761 cgcgccgctc gccgggctga gctcgcgcca cggcacgttt ttcgtcaccg gcaaccacga
     2821 gtactactcc ggcgcgccgg cctgggtcgc cgagctgcgc cgcattggcc tgcgcgtgct
     2881 gatgaacgag catgtcgtgc tcgagcacgc cggtgcggcg ctgctggtcg gcggcgtgac
     2941 cgactttcac gcccaccact acgatccggc gcagcgcagc gacccgcagg cggcgttggc
     3001 gggcgcgccc acgcacgcga gcacgcgcgt gctgctcgcg catcagccgc gcagcgccga
     3061 agcggccgag cgcgccggct tccagctgca gctgagcggc cacacgcacg gcggccagtt
     3121 cctgccctgg aacctgttcg tgcggctgca gcagccgttc accgccgggc tgcatcggct
     3181 gcggcggatg tgggtctaca cgagtcgcgg caccggctac tgggggccgc cgaagcgctt
     3241 cggcgcgccg tcggagatca cccgcctgcg cctcgtgagc gtctgacctt cgtcacgaac
     3301 tcggccagcc ccgccacgcg cgtgtggtgc ccctgccaca cggcggccgg aacgctgcgg
     3361 gttttcccgc agaa
//
LOCUS       DATMJB010000318         2365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_614782, whole genome shotgun sequence.
ACCESSION   DATMJB010000318 DATMJB010000000
VERSION     DATMJB010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614782"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="VNU48_06920"
     CDS             complement(<1..453)
                     /locus_tag="VNU48_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HWI11045.1"
                     /translation="MGPEPLPLAPAAIRALAGIATATLTTVLLQRGLRNVWLRGTRPL
                     APGQPRVVGRAFTLRFVPAREDLATPASWGAPISTRAAIEAMPAGCVAVVGAMGVTDA
                     GIFGDILCARMKKRGVAGLVSDGVVRDLAGVLGTGLPVWCQGTAAPASV"
     gene            566..952
                     /locus_tag="VNU48_06925"
     CDS             566..952
                     /locus_tag="VNU48_06925"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI11046.1"
                     /translation="MLQKSPMYAYIPAQDVARARQFYEGKLGFVPKEELGGGVAYAFA
                     GQTACFLYPTPNAGTSKASQAFWQVDDVDREIDELKARGVVFEDYDMPGEKSASGALT
                     AGGAKAAWFKDSEGNILALIQTLGSR"
     gene            1120..1749
                     /locus_tag="VNU48_06930"
     CDS             1120..1749
                     /locus_tag="VNU48_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11047.1"
                     /translation="MSSDLSTPVLLRRIAQLEATVGRLSDDLGSLRSAVSKQGLQVPS
                     ARGVRARVPRAGSSITDSTSQTRAAGGLAGAAARGEAARVQWVKDGLVVPGEQLAQAW
                     GLTRQALAPAADRGEVFAVKVGNRLYYPQAFLGMDRETVATVCRALGDLGAGEKLMFW
                     LRNHGALAGMSVAAALESGVPVPKVERLASAWARERGAAHGARNAAAAS"
     gene            1718..2329
                     /locus_tag="VNU48_06935"
     CDS             1718..2329
                     /locus_tag="VNU48_06935"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HWI11048.1"
                     /translation="MALETQLRLPEPGSFEDLLRPVRIEVASLVRLSRHPATEPYWSA
                     GVYRFDDPDPGREGAFGTCYTASTVEVAFAESVIHESGRFASRRYEVPAAELTERFVV
                     RFTCGKRKTLVLADLTGAALKSLGLNNDISALADYSASQAWAQAIHGAGSRWDGIRYV
                     SRQMNKGSAYAIFERSGLRKLRAEKLKSRQVDDLCDRFNVAAV"
BASE COUNT          368 a          795 c          841 g          361 t
ORIGIN      
        1 caccgaggcc ggcgcggccg tgccctggca ccacaccggc agcccggtgc cgagcacgcc
       61 ggcgaggtcg cgcacgacgc cgtcgctgac caggcccgcg acgccgcgct tcttcatgcg
      121 cgcgcacagg atgtcgccga agatgccggc gtcggtgacg cccatcgcgc cgaccaccgc
      181 gacgcagccg gccggcatcg cctcgatcgc ggcgcgcgtg gagatcggcg cgccccacga
      241 ggcgggcgtc gcgaggtctt cgcgcgccgg cacgaagcgc agcgtgaagg cgcgaccgac
      301 gacgcgcggc tggcccggcg cgagcggccg cgtgccgcgc aaccagacgt tgcgcaggcc
      361 gcgctgaagc agcacggtcg tgagcgtcgc ggtcgcgatg ccggccaggg cacggatcgc
      421 ggcgggggcg agcggaagcg gttcggggcc catcgaaatc gtcctgtggc ggctgcggtc
      481 tgtcgattct gcatcgcccc gcgcgtcatg atcagaccag cccggtgcgc gacgttcgcg
      541 ccgattcacc cgaaggagac ccgcgatgct gcagaaatcc ccgatgtacg cctacattcc
      601 cgcgcaggac gtggcgcgtg cgcgccagtt ctacgaaggc aagctcggct tcgtgccgaa
      661 ggaagaactc ggcggcggcg tcgcctacgc gttcgccggg cagaccgcct gcttcctgta
      721 cccgacgccg aacgccggga cctcgaaggc gagccaggcg ttctggcagg tcgacgatgt
      781 cgaccgcgag atcgacgagc tgaaggcgcg cggcgtcgtg ttcgaggact acgacatgcc
      841 gggcgagaaa agtgccagcg gcgcgctcac ggccggcggc gcgaaggcgg cttggttcaa
      901 ggacagcgag ggcaacatcc tggcgctcat ccagacgctc ggttcgcgct gagcgcggcc
      961 ggccgccgtc tcaatttatc gtggcagcaa agacccgccc ttcgcggacg cgcatctcga
     1021 gtcgatgcgg cgcctgatgg cgcacatgtc gcgcggcccg agcgatttgc tacattgtct
     1081 gcgtttgctt cgatgtcaga atttgcttaa gatgtacaca tgagcagcga tctcagcacc
     1141 cccgtcctgc tgcgccgcat cgcgcagctc gaagccactg ttggccgcct cagcgacgac
     1201 ctcggcagcc ttcgctcggc ggtcagcaag caggggttgc aggtgccgag cgcgcgaggc
     1261 gtccgggcgc gggtgccacg tgccgggtcc tccatcacgg acagcacctc gcaaacccgg
     1321 gctgccggcg gcttggctgg tgccgctgcg cgcggcgagg cggcgcgtgt tcagtgggtc
     1381 aaggatggcc tcgtcgtgcc cggcgaacag cttgcacagg cctggggcct cacgcgccag
     1441 gccttggcgc ccgccgcgga ccggggcgag gtcttcgcag tgaaagtcgg caatcggctg
     1501 tactacccgc aggcgttcct gggtatggat cgcgagaccg tggccacggt ctgccgtgca
     1561 cttggtgacc tcggcgccgg cgagaagctg atgttctggc tgcgcaacca cggtgctctg
     1621 gcaggcatga gcgtcgcggc cgccttggag tcgggagttc ccgtgcccaa ggtcgagcgg
     1681 ctggcttcag cctgggcgcg cgagcgcgga gccgcgcatg gcgctcgaaa cgcagctgcg
     1741 gcttcctgag cccggatctt tcgaggacct gcttcgaccg gtcagaatcg aagtggcgtc
     1801 gctcgtgcgg ctgagccggc acccggccac cgagccttac tggtcggcag gcgtctaccg
     1861 cttcgatgat ccagatcccg gccgggaagg cgcgttcggc acgtgctaca ccgccagcac
     1921 cgtcgaggtg gccttcgcgg aaagcgtcat ccatgagtcc gggcgattcg caagccgccg
     1981 ctacgaggtg ccagccgccg aactgaccga gcgcttcgtc gtgcgcttta cctgtgggaa
     2041 acgcaagacg ctggtcttgg ccgacctgac cggggctgcg ctcaagtctc tgggtctgaa
     2101 caacgacatc agtgcgttgg ccgactactc ggcgagccaa gcttgggcgc aggccattca
     2161 cggcgcgggt tcccgctggg acgggatccg ctacgtgtcg cgacagatga acaaggggtc
     2221 tgcctatgcg atctttgagc gcagcggact tcggaagctg cgagcggaga agctcaagtc
     2281 gcggcaggtg gatgacctct gcgatcggtt caacgtcgct gcggtctgac ggcgccgccc
     2341 ggcggaccag aaggtgcccc cgagc
//
LOCUS       DATMJB010000319         3583 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_382169, whole genome shotgun sequence.
ACCESSION   DATMJB010000319 DATMJB010000000
VERSION     DATMJB010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382169"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..604
                     /gene="arfB"
                     /locus_tag="VNU48_06940"
     CDS             185..604
                     /gene="arfB"
                     /locus_tag="VNU48_06940"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005014247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HWI11049.1"
                     /translation="MLRISRDLTIDENDIDISFVRASGPGGQNVNKLSTAAQLRFDTG
                     RIALAPDAEARLKGLAGQRMTKDGVIVIHAQRFRTQERNRADAIDRLLELLREAMVRP
                     TPRRATRPTLGSKQRRLEGKKRRSDVKAKRGSARFDD"
     gene            710..2521
                     /gene="mutL"
                     /locus_tag="VNU48_06945"
     CDS             710..2521
                     /gene="mutL"
                     /locus_tag="VNU48_06945"
                     /inference="COORDINATES: protein motif:HMM:NF000953.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWI11050.1"
                     /translation="MPVRQLPEQIVNRIAAGEVVERPASVVKELVENAIDAGASRIDI
                     FTDGGGRRRIGITDDGSGMPHADLALAVDRHATSKLDDEDLLRIRTLGFRGEALPSIG
                     AVARLGITTRHASEPHAWSLSVEGGEKSPIMPAALSQGTRVEVSDLFYATPARLKFLK
                     TDRTEAEAIREVVRRLAMARPDIAFTLAGEERAPVTWAAALPGAAGRLTRLGDILGAD
                     FRGSAIEVHSERDGVVVAGFAAAPSLTRANALGQYLFVNGRPVRDKLILGAVRAAYSD
                     YLPRDRHPVVALFVTLDPQEVDANVHPAKTEVRFRNAGLVRALIVHALKDGLAREGKR
                     TAANSDGAALASFRPSFAPRPNDWDWRRSPAYPTGPISGFEGAAATAFAEPGQAAFDV
                     GTPTADVRFQETPSAELLDRPLGAARTQIHETYIVSQTRDGLVVVDQHAAHERIVYEK
                     LKASLARNGVQRQILLIPEIVELDEATVERLVDRAEELASFGLAIDSFGPGAVAVRET
                     PSLLGKANAGSLLRDLAEHMAEWDEALPLERRLMHVAATMACHGSVRAGRRLKPEEMN
                     ALLREMEDTPNSGQCNHGRPTYVELKLADIEKLFGRR"
     gene            complement(2668..3222)
                     /gene="rsmD"
                     /locus_tag="VNU48_06950"
     CDS             complement(2668..3222)
                     /gene="rsmD"
                     /locus_tag="VNU48_06950"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWI11051.1"
                     /translation="MRVVGGRLKGRNLASPSSQAIRPTADRLRESVFNILIHAYDNPI
                     EDARVLDLFAGTGALGIEAVSRGAAFTLFVDNGAEARALLRNNVEALGLGGVTKVYRR
                     DATNLGPAHPMEPFTLVFLDPPYRMQLAEKALASLRDGGWLTPGALLVVEEAKAAAFA
                     APDGFEELERRAYDDTEFVFLRGG"
     gene            complement(3254..>3583)
                     /locus_tag="VNU48_06955"
     CDS             complement(3254..>3583)
                     /locus_tag="VNU48_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11052.1"
                     /translation="QVEEVKTRVLREQLGEKIATLAGADFNRPMPGDANEEPADDNAP
                     RGKKPFKPAGKSGLIADRKGRRVLVQRTGSEEARARNEAEANGFGPPRRPQRGYHGKR
                     DLKPRDE"
BASE COUNT          596 a         1242 c         1173 g          572 t
ORIGIN      
        1 tacaccgcgg agccgggcgc tctacgtcca cagcgataga ccccggaccg gcagcgcacc
       61 acgccccgga agctgctgcg cagcatcggg gggcacctgc cctctccttc agcgtccctg
      121 gtaaaaccgc tatcctccac cagcctgatt cccgttgggg gaatatcggg ccgccggtga
      181 caccatgctg cggatttccc gcgacctcac aatcgacgag aacgacatcg acatcagttt
      241 cgtccgcgcc tccggtccgg gcggacagaa cgtcaacaag ctctcgaccg ccgcgcaatt
      301 gcgtttcgac actggccgca tcgcgctggc gcccgacgcc gaggcgcggc tcaaggggct
      361 ggccggccag cgcatgacca aggacggcgt catcgtgatc catgcccagc gcttccggac
      421 ccaggagcgc aatcgcgccg acgccatcga ccggctgctc gaattgctgc gcgaggccat
      481 ggtccggccg accccgcgcc gcgccaccag acctactttg ggttcgaagc aacgccggct
      541 ggaaggcaag aagcgccgca gcgacgtcaa ggcgaaacgc ggctcggctc gctttgacga
      601 ttgaggcgaa aatcctgttc gtgaacaacg gggagagccc caggcccgcc ctcccatatc
      661 gggcttaaag cgcctagatt gaggctcggt ttccacgagt cgcctgccta tgcccgtccg
      721 ccagctcccg gaacagatcg tcaaccgcat cgccgccggc gaagtggtcg aacgccctgc
      781 gagcgtggtc aaggaactgg tggaaaatgc catcgatgcc ggcgccagcc ggatcgacat
      841 tttcaccgat ggcggcggcc ggcggcggat cggcatcacc gacgacggca gcggcatgcc
      901 tcatgccgat cttgcgctcg cggtcgatcg tcatgccacc tcgaagctcg acgacgagga
      961 cctgctgcgg atccgcacgc tcgggttccg cggcgaagcg ctgccctcga tcggcgccgt
     1021 ggccaggctc ggcatcacca cgcgccacgc cagcgagccg catgcgtggt cgctgtcggt
     1081 cgaaggcggc gagaaatcgc cgatcatgcc ggcggcgctc agccaaggca cccgcgtcga
     1141 ggtcagcgac cttttttatg cgacgcccgc acggctgaag tttctgaaga ccgaccgcac
     1201 cgaagccgaa gcgatccgcg aagtggtgcg ccgcctcgcg atggcgcggc cggacatcgc
     1261 cttcacgctg gccggagagg aacgcgcgcc ggtcacctgg gccgcggcgc tgccgggcgc
     1321 tgccggccgg ctgacccggc ttggcgacat attgggtgcg gattttcgcg gcagcgccat
     1381 cgaagtgcat tccgaacgcg atggcgtcgt ggtcgcgggc ttcgccgcgg ccccctcgct
     1441 gacgcgcgcg aatgcgctcg ggcaatatct gttcgtcaac ggtcgcccgg tgcgcgacaa
     1501 gctcattctg ggcgcggtgc gcgcggccta ttcggattat ctgccgcgcg accgccatcc
     1561 ggtggtggcg ctgttcgtga cgctggatcc gcaggaggtc gacgccaatg tgcatccggc
     1621 caaaaccgag gtgcgcttcc gcaacgccgg gctggtgcgc gcgctgatcg tgcatgcgct
     1681 aaaagacggc ctcgcccgcg aaggcaagcg caccgccgcc aacagcgatg gtgcggcact
     1741 cgcctcgttc cgcccgtcat tcgcgccgcg cccgaacgac tgggactggc ggcgttcgcc
     1801 ggcttatccc acgggcccga tatccggctt cgagggcgcc gcggcgacag cctttgccga
     1861 accgggacag gccgccttcg acgtcggcac gcccaccgcc gacgttcgct ttcaggaaac
     1921 gccgtccgcc gagttgctcg accgcccgct cggtgctgcg cggacgcaga ttcacgagac
     1981 ctatatcgtg tcgcagaccc gcgacgggct cgttgtggtg gaccagcacg ccgcgcatga
     2041 gcgcatcgtc tacgagaaac tgaaagcctc gctggcgcgc aacggcgtgc agcggcagat
     2101 cctgctgatc ccggaaatcg tcgagctgga cgaggcgacg gtagagcggc tggtcgatcg
     2161 cgccgaggag ctggcatcgt ttggcctcgc gatcgactcc ttcggccccg gcgcggtcgc
     2221 ggtgcgcgaa acgccgtcgc tgctcggcaa ggccaacgcc ggatcgctgc tgcgcgatct
     2281 cgccgaacac atggcggaat gggacgaggc gttgccgctg gagcgtcgcc tgatgcatgt
     2341 cgcggccacc atggcctgcc acggctcggt gcgcgccggc cgccggctca agcccgaaga
     2401 gatgaacgcg ctgttgcgcg agatggaaga cacgccgaat tccggccagt gcaaccacgg
     2461 ccgccccacc tacgtggaat tgaagctcgc ggatatcgag aagctgttcg ggcgtcgatg
     2521 agggcgtcat ccataacgac ttcgccatta tcgctggagg tgctcgccgt ctgcggcgag
     2581 cctcgaagga tgacaagcgc cggcgttcgt tgcgcatcct tcgaggtacg cggagcgcgc
     2641 acctcaggat gacgctgcgg aaggcgttta tccaccccgc aagaacacaa actccgtatc
     2701 gtcatacgcc cgccgctcca gctcctcgaa gccatcggga gccgcgaacg ccgcggcctt
     2761 cgcttcctct accaccagca gcgcgccagg cgtcagccag ccgccatcgc gcagcgaggc
     2821 cagcgccttt tcggcgagtt gcatgcgata gggcggatcg agaaagacca gcgtgaacgg
     2881 ctccatcgga tgcgccgggc cgagattggt ggcgtcgcgg cgatacacct tggtcacgcc
     2941 acccaggccc agcgcctcga cattgttgcg caacagtgcg cgggcttcgg cgccgttgtc
     3001 gacgaacagc gtgaatgccg cgccgcgcga aacagcctcg atcccgagcg ccccggtgcc
     3061 ggcgaacagg tcgagcacgc gcgcgtcttc aatcggattg tcataggcgt gaatcaggat
     3121 attgaaaacc gactcgcgca accggtccgc cgtcgggcgg atggcctgcg acgagggcga
     3181 tgcgaggttg cggcctttca gccgtccgcc gacgacgcgc acagctaggc cccgggtgtg
     3241 aatttcgaca tgttcactcg tcgcgcggct tcaggtcgcg cttgccgtga tagccgcgct
     3301 gcggacgccg cggcgggcca aaaccattgg cctcggcttc gttgcgcgcg cgggcttcct
     3361 cgctgccggt acgctgcacc agcacgcgac gacctttgcg gtccgcgatc agcccgctct
     3421 tgccggccgg cttgaacggc ttcttgccac ggggggcatt gtcatcggct ggctcctcgt
     3481 tggcgtcgcc cggcatcggc cgattgaaat cggcaccagc gagtgtcgcg atcttttcgc
     3541 cgagctgttc gcgcagcacg cgggtcttga cctcctcgac ctg
//
LOCUS       DATMJB010000320         2198 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_786574, whole genome shotgun sequence.
ACCESSION   DATMJB010000320 DATMJB010000000
VERSION     DATMJB010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_786574"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..781)
                     /gene="pyrE"
                     /locus_tag="VNU48_06960"
     CDS             complement(104..781)
                     /gene="pyrE"
                     /locus_tag="VNU48_06960"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWI11053.1"
                     /translation="MSSNDTEDALAQDFVKFAVEAGVLRFGEFRTKAGRLSPYFFNSA
                     LFDDGAKLARLAEFYARRLLASGCAFDMLFGPAYKGITLAAATAVALAGLGRNVPFAF
                     NRKEAKAHGEGGTLVGAPVRGRVVIVDDVITDGASKRESVELLRAAGGAPAAVLIALD
                     RMERGGTDAQLSERSAVEDFEREYGLPVISIATVQDLLQYLRSNADPALGTHFARVAA
                     YRERYGV"
     gene            797..1582
                     /locus_tag="VNU48_06965"
     CDS             797..1582
                     /locus_tag="VNU48_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWI11054.1"
                     /translation="MFRLVSLNLNGIRSAANKGFEAWAEGVAADCMGVQEVKAQADTV
                     VGRFDTVAGMTGHFHYAEKKGYSGVGLYTRQRPSEVVLGIGNAEFDAEGRYVEARFDT
                     SRSKLSIISCYFPSGSSGELRQAAKFRFLALMAPHLKALASERELILVGDINIAHKEI
                     DLKNWRGNQKNSGFLPEERAWMTSLLDEIGLVDVFRTLNPHPEQYTWWSNRGQAWAKN
                     VGWRLDYHLATPAIARHAKAEHIYLEQRFSDHAPLLIDYDFTL"
     gene            1650..>2198
                     /locus_tag="VNU48_06970"
     CDS             1650..>2198
                     /locus_tag="VNU48_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HWI11055.1"
                     /translation="MSLETIVIETAPNPTASIIILHGLGADGNDFAPIAQELDLSSVG
                     PVRFVLPSAPARPVTINGGYVMPAWYDILGPGRPEDAAGLRASQAQVDALIGAEIARG
                     IPAARIVLAGFSQGCAMTFMTGLRYGERLAGLLGMSGYLPLAELTGAERSAANAGVPI
                     FQAHGSADEVIPIARATWSRDAL"
BASE COUNT          355 a          790 c          747 g          306 t
ORIGIN      
        1 agatcgagcg gccgctggtg gtctgggccg aggtcagcgc aggcgcaccg agcgccagca
       61 acacgacgca ggtctcgaag gctcgcacac ggatggcttt cattcagact ccataacgtt
      121 cacggtaggc ggcgacgcgg gcaaagtgtg tgccgagcgc cgggtcggcg ttcgagcgca
      181 ggtactgcaa caggtcctgc acggtcgcga tcgagatcac cggcaggccg tactcgcgct
      241 cgaagtcctc gaccgccgag cgctcggaga gttgcgcgtc ggtgccgccg cgctccatgc
      301 ggtcgagcgc gatcagcacc gccgccggcg cgccgccggc cgctcgcagc agctcgaccg
      361 actcgcgctt ggaggcgccg tcggtgatca cgtcgtcgac gatcacgacg cgcccgcgca
      421 ccggtgcgcc gacgagcgtg ccgccttcgc cgtgcgcctt cgcctccttg cggttgaacg
      481 cgaacggcac gttgcggccc agccccgcga gcgccaccgc ggtcgccgcg gccagcgtga
      541 tgcccttgta ggcggggccg aacagcatgt cgaatgcgca gcccgatgca agcaggcgcc
      601 gcgcatagaa ttccgccagc cgcgccagct tggcgccatc gtcgaacagg gccgagttga
      661 agaagtacgg cgagagccgg ccggccttgg tcctgaactc accgaagcgc aacacgccgg
      721 cctcgaccgc gaacttcacg aagtcctgcg cgagtgcgtc ctcggtgtcg ttgctgctca
      781 tcccgaaagt cctgtcgtgt tccgtctcgt gtcgctgaat ctgaacggca tccgctcggc
      841 ggccaacaag ggcttcgagg cctgggccga gggcgtggcg gccgattgta tgggcgtgca
      901 ggaggtcaag gcgcaggccg acacggtggt cggccggttc gacacggtcg ccggcatgac
      961 cgggcatttc cactacgccg agaagaaggg ctactcgggc gtcggcctgt acacgcgcca
     1021 gcggccgagc gaggtggtgc tcggcatcgg caacgccgag ttcgacgccg agggccgcta
     1081 tgtcgaggcg cgcttcgaca cctcgcggag caagctcagc atcatcagct gctacttccc
     1141 gagcggctcc agcggcgagc tgcggcaggc cgccaagttc cgcttcctcg cgctgatggc
     1201 gccgcacctg aaggcgctgg cctcggagcg cgagctgatc ctggtcggcg acatcaacat
     1261 cgcgcacaag gagatcgacc tgaagaactg gcgcggcaac cagaagaaca gcggcttcct
     1321 gcccgaggag cgcgcctgga tgacgagcct gctcgacgag atcggcctgg tcgacgtgtt
     1381 ccgcacgctg aacccgcatc ccgagcagta cacctggtgg agcaaccgcg gccaggcctg
     1441 ggccaagaac gtcggctggc gcctcgacta ccacctcgcc acgccggcga tcgcgcggca
     1501 cgcgaaggcc gagcacatct acctcgagca gcgcttcagc gaccatgcgc cgctgctgat
     1561 cgactacgac ttcacgctct gaccgccggg cgccgcggcg acaatcgatc gcgcccctcc
     1621 acccacccgc gacacctgcg agcccgacca tgtccctcga aacgatagtg atcgaaaccg
     1681 cgccgaaccc gacggcgagc atcatcatcc tgcacggcct gggcgccgac ggcaacgact
     1741 tcgcgccgat cgcgcaagag ctcgacctga gcagcgtcgg cccggtgcgt ttcgtgctgc
     1801 ccagcgcgcc ggcccggccg gtgacgatca acggcggcta cgtgatgccg gcctggtacg
     1861 acatcctcgg ccccggccgg cccgaagacg ccgcggggct gcgcgcctcg caggcccagg
     1921 tcgacgcgct gatcggcgcc gagatcgcgc gcggcatccc ggccgcacgc atcgtgctgg
     1981 ccggcttctc gcagggctgc gcgatgacct tcatgaccgg cctgcgctac ggcgagcggc
     2041 tggccgggct gctcggcatg tcgggctacc tgccgctggc cgagctcacc ggcgccgagc
     2101 gcagcgcggc caacgccggg gtgccgatct tccaggccca cggcagcgcc gacgaggtga
     2161 tcccgatcgc gcgcgcgacc tggtcgcgcg acgcgctg
//
LOCUS       DATMJB010000321         3116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_340949, whole genome shotgun sequence.
ACCESSION   DATMJB010000321 DATMJB010000000
VERSION     DATMJB010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340949"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..955
                     /locus_tag="VNU48_06975"
     CDS             80..955
                     /locus_tag="VNU48_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HWI11056.1"
                     /translation="MNPTETIREALPADPVARRAAARRVYRHRLPVRLMHWVNVVCLT
                     ILLMSGLTIFNAHPALYWGRDSNFADPWVSIGAVRAPGGGIQGVTTIAGHRFATDGVL
                     GASTVGDATTKSARAFPSWATIPGPQWLSMGRHWHFFFAWLFVVNGVAYVLYAVVSGH
                     LKRDLLPTRAELRRIGSSIVDHLRFRHPAGAAAARYNVLQNIAYLVVIFGLLPLVIVA
                     GMAMSPRLDAVFPGWVDLLGGRQSARTLHFLAATGLLLFVLVHLFEVLVNGAWNQIRS
                     MVTGWYVLPAEKEAK"
     gene            952..1761
                     /locus_tag="VNU48_06980"
     CDS             952..1761
                     /locus_tag="VNU48_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HWI11057.1"
                     /translation="MTIRKPIASGRRVAPSRRSALRSALASVGGLALAGCDRLSNNHA
                     FVDVLKSAQQLSHGAQRVVAGRKAMAQEFTQADVAAHFKANGTLVPADADYQALMRGQ
                     FADWKLQVDGLVEKPAAFTLAELRAMPSRTQITRHDCVEGWSTIGKWKGVPLSHVLDR
                     VRPLPNAKYVVFRCADSMESPSAGRDSRYYESVDMDDAYHVQTVLAYELNDQPLPVNN
                     GAPLRVRIERQLGYKHAKYLMRLELVERFDQIRGGLGGYWEDNGYEWYAGI"
     gene            complement(1867..2364)
                     /locus_tag="VNU48_06985"
     CDS             complement(1867..2364)
                     /locus_tag="VNU48_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11058.1"
                     /translation="MRPVTSAARFVAAGLAALAAGVASAPVNAQEFGVYLACTGKLHA
                     KGRAVPAHLDLALRRNSQLALIQRSDVLPVGERMKLEISPALYSMVYAAPLRNSTVWY
                     DWIRGSVFVWDPDLLKLQSVRLSVDRQTAALEGEMRDGAGVQLARLAMRCDPKNNDTV
                     PEPKF"
     gene            complement(2361..>3116)
                     /locus_tag="VNU48_06990"
     CDS             complement(2361..>3116)
                     /locus_tag="VNU48_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HWI11059.1"
                     /translation="VSGLCFSTPAAAEDAPPVMEKCDKTLGVVRIQEDTGAEWYRYYV
                     GRLGSTAPVISAMVMESNCFVVVERGTGERDINAETQRARGDEARESDTRGKGQQVVA
                     DFLLKPEIVMNKKGDSGGNFGGIGKMLPGALGNVIGAGRVGFSSNEAATVMTLVDIRS
                     TVRLVAAQGHSESKNFNFSGLGFGRFGVGAGSAYSNTDEGKMVTAAFVDSYNKLVVAV
                     RNYKAQTVEGGLGNGGGLGVQGGITAASKKVGK"
BASE COUNT          463 a         1092 c         1055 g          506 t
ORIGIN      
        1 gatgacgacc atgccctgac gcgtgcgcgc aaacaggtgc accatgtaag gatcgcgacg
       61 ccgcgccgac ccagctgcca tgaacccgac cgagacgatt cgcgaggcct tgccggccga
      121 cccggttgcg cggcgcgcgg cggcacgccg cgtctaccgc caccgcctgc cggtgcggct
      181 gatgcactgg gtcaacgtcg tctgtctgac gatcctgctg atgagcgggc tgacgatctt
      241 caacgcgcac ccggcgctgt actgggggcg cgattcgaac ttcgccgacc cctgggtctc
      301 gatcggcgcg gtgcgcgcgc cgggcggcgg catccagggc gtcacgacga tcgccggcca
      361 ccgcttcgcg accgacggcg tgctcggcgc gtcgaccgtc ggcgacgcca cgaccaagag
      421 cgcgcgcgcg ttcccgtcgt gggcgacgat ccccgggccg cagtggctgt cgatggggcg
      481 gcactggcac ttcttcttcg cctggctgtt cgtcgtcaac ggcgtcgcct acgtgctgta
      541 tgccgtggtc agcggccacc tgaagcgcga cctgctgccc acgcgcgccg aactgcgccg
      601 catcggctcg tcgatcgtcg accacctgcg gttccggcat cccgcgggcg ccgcggccgc
      661 gcgctacaac gtgctgcaga acatcgcgta cctggtcgtg atcttcgggc tgctgccgct
      721 ggtgatcgtt gccggcatgg cgatgtcgcc gcggctggat gcggtgttcc cgggctgggt
      781 cgatctgctc ggcgggcgcc agtcggcgcg cacgctgcac ttcctcgccg ccaccgggct
      841 gctgctgttc gtgctggtgc acctgttcga ggtgctggtg aacggcgcgt ggaatcagat
      901 ccgatcgatg gtcaccggct ggtatgtgct gccggccgag aaggaggcga aatgacgatt
      961 cgcaaaccga tcgccagcgg gcgccgcgtc gcacccagcc gccgcagcgc gctgcgcagc
     1021 gcgctcgcgt cggtcggcgg cctggcgctg gccggctgcg accggctgtc gaacaaccat
     1081 gcgttcgtcg acgtgctgaa gagcgcgcag cagctcagcc acggcgcgca gcgcgtggtc
     1141 gcgggtcgca aggcgatggc gcaggagttc acccaggccg acgtcgccgc gcacttcaag
     1201 gccaacggca cgctggtgcc cgccgacgcc gattaccagg cgctgatgcg cggccagttc
     1261 gccgactgga agctgcaggt cgacggtctg gtcgagaagc ccgcggcgtt cacgctcgcc
     1321 gagctgcgcg cgatgccgtc gcgcacccag atcacgcgcc acgattgcgt cgaaggctgg
     1381 agcacgatcg gcaagtggaa gggcgtgcca ctgagtcacg tgctcgaccg ggtcaggccc
     1441 ttgccgaacg ccaagtacgt ggtgttccgc tgcgccgatt cgatggagtc gccgagcgcg
     1501 ggccgtgatt ccagatacta cgaaagcgtc gacatggacg acgcctatca cgtgcagacc
     1561 gtgctcgcat acgagttgaa cgaccagccg ctgccggtca acaacggcgc gccgctgcgc
     1621 gtgcgcatcg agcgccagct cggctacaag cacgcgaaat acctgatgcg gctcgagctg
     1681 gtggagcgct tcgaccagat ccgcggtggc ctgggcggct actgggaaga caacggctac
     1741 gagtggtatg cgggcatttg aagcggggtg atcgcgagcg gcggcgagac ctgggtcgtc
     1801 gagcattcgc ccgatcggct gaccgagttg acgacgcggt tctgaatcgg gccgcagccg
     1861 gcggcgctag aacttcggct ccggcaccgt gtcgttgttc ttcgggtcgc agcgcatcgc
     1921 cagccgcgcc agttgcacgc cggcgccgtc gcgcatctcg ccttcgagcg ccgcggtctg
     1981 ccggtcgacc gacagcctga ccgactggag cttgagcaga tcaggatccc acacgaacac
     2041 cgagccacgg atccagtcgt accagaccgt gctgttgcgc agcggcgccg cgtacaccat
     2101 cgaatacaag gccgggctga tttccagctt catccgttcg ccgaccggca gcacgtcgga
     2161 gcgctggatc agcgccagct ggctgttgcg ccgcagcgcg agatcgaggt gcgccggcac
     2221 cgcgcggccc ttggcgtgca gcttgccggt gcaggcgagg tagacgccga attcctgtgc
     2281 gttgaccggc gccgaggcca ccccggcagc cagcgccgcg aggcccgcgg cgacgaagcg
     2341 cgccgcgctt gtcactggcc tcacttgccg accttcttcg atgccgccgt gatgccgccc
     2401 tggacgccca gcccgccgcc gttgcccagg ccgccctcga ccgtctgtgc cttgtagttg
     2461 cggaccgcga cgacgagctt gttgtaggag tcgacgaacg ccgcggtcac catcttgccc
     2521 tcgtcggtat tcgagtaggc gctgccggcg ccgaccccga aacgcccgaa ccccaggccg
     2581 gagaagttga aattcttgct ctccgagtgc ccctgcgccg cgaccagacg caccgtgctg
     2641 cggatgtcga ccagcgtcat gacggtcgcc gcctcgttcg agctgaaccc gacgcggccc
     2701 gcgccgatca cgttgccgag cgcgccgggc agcatcttgc cgataccgcc gaaattgccg
     2761 ccgctgtcgc ccttcttgtt catcacgatc tcgggcttga gcaggaagtc ggcaacgacc
     2821 tgctgaccct tgccgcgggt gtcgctttca cgcgcctcgt caccgcgggc gcgctgcgtc
     2881 tcggcgttga tgtcgcgttc gcccgtgccg cgctcgacga ccacgaagca gttcgattcc
     2941 atcaccatcg cgctgatcac cggcgcggtc gacccgagcc ggccgacgta gtagcggtac
     3001 cactctgcgc cggtgtcttc ctggatgcgc acgacgccga gcgtcttgtc gcatttctcc
     3061 atcaccggcg gcgcatcctc ggccgcggcc ggggtcgaga aacagaggcc cgagac
//
LOCUS       DATMJB010000322         4543 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_303963, whole genome shotgun sequence.
ACCESSION   DATMJB010000322 DATMJB010000000
VERSION     DATMJB010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303963"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VNU48_06995"
     CDS             complement(<1..202)
                     /locus_tag="VNU48_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11060.1"
                     /translation="MDAFLVAAMAGATLAPDYRDVCGAAAPAAKFAPRLILYPRATSR
                     SDGGKQAVRQGTPRAEGGARQRH"
     gene            209..1888
                     /locus_tag="VNU48_07000"
     CDS             209..1888
                     /locus_tag="VNU48_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HWI11061.1"
                     /translation="MIHLLNLLAAIALLVWGTNIVRTGVIRMFGEKLRSFLASSLSNR
                     FKAVLAGLAVTGLVQSSTATAVIISSFVGSGLVGLTSGLAVMLGAEVGTSLMTVVFSF
                     DLSWLSPLLIFIGVVLLIANEKSTAGRFGQVAIGFGLITLGLQLIVSATQPLIASAAF
                     RGLLVALPNDVFLDIVVGALLTMATYSSLAFVLLTATLAASGILPVTIALGLVLGANI
                     GSGVLAMLTSSGPPVVRLRLPLGSLLFKLAGCVIAIPLLSQIHVLLQDCIPTVHAQVI
                     AFHLGFNAVRALLFIGLTAPMARLVEKLLPAPPTQIGPARPRHLDPSALATPSLAISC
                     AAREALHQADVVETMLGGILPVLRDNDLVLAEQLRNLDDTVDELYSAIKFYLTQISRE
                     ALSEGEGRRGTDIVSFTINMEQIGDIIERVIQDIEDKKIRKNRRFSDAGMAEIVHLHE
                     RLTANLRLGMSVFLDGHVRDAKKLLEEKARFRDLEHEYAASHIARLQDHTANSIETSS
                     LHIDLISELKRINSHICSIAYPILESAGALTKTRIRSSQHGALSGPEERSF"
     gene            1888..2772
                     /locus_tag="VNU48_07005"
     CDS             1888..2772
                     /locus_tag="VNU48_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWI11062.1"
                     /translation="MTAPLALTWPVVLAVLGGALLHASWNALIKSGHDKALDTALLHF
                     LGALIALPFMLWVGPPPAAAWPFIAASLAIHIGYYVALVGAYQHGELGLTYPIMRGFA
                     PMLVALASGAFIGEAPTLAAWLGIAAVTLGVALVGLAHPGEALHHRKALLFAFANAAI
                     IALYTVVDGRGVRAAAVDDHGALRYVLMLFVLDGIAYPGFVWLRRSAAGRREIVAYAR
                     RRWPVAALGGSASIGSYAIALWAMTRAPVASVAALRETSVLFAAVLGTVLLKERFGLQ
                     RAFGTLVIVAGVMALRFG"
     gene            complement(2833..>4543)
                     /gene="ligA"
                     /locus_tag="VNU48_07010"
     CDS             complement(2833..>4543)
                     /gene="ligA"
                     /locus_tag="VNU48_07010"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWI11063.1"
                     /translation="FDGLAINLRYERGELVQAATRGDGETGEDVTHTVRMIATVPKRL
                     RGAAAAQAAVIEVRGEVFMRRDDFEALNERQRKLIESGARGEKTFVNPRNAAAGVVRQ
                     LDAGKALRRPLSFFAYGLGEVDGWALPPTQGELLAALRRLGVPVSDESAVVHGAAGLI
                     DYHARIAARRDALPFDIDGVVYKVDDRALQATLGFKSREPRWACAHKYPAQEQTTVVR
                     DIDIQVGRTGKLTPVAKLEPVFVGGTTVSNATLHNEDETRRKDVRIGDTVIVRRAGDV
                     IPEVVGVVLERRPAQVGEPFDLYKRLAGKCPICASAITREEGEVDWRCSGGLFCPAQR
                     KQAILHFAQRRALDIEGLGDKLVDQLVDAGIVGTLPDLYKLGLANLTALDRMGKKSAV
                     NLLAGLEASKRTSFARFLYGLGIRHVGETTAKDLAKHFGNIDRLMAASVERLLEVNDV
                     GPIVALSVRTFFDQPHNVEVVEQLRAAGIEWPETDGAADAAPRPLAGKTFVLTGTLPG
                     LSRDDAKDRIEAAGGKVSGSVSKKTHFVVAGAEAGSKLDKANELGIAVLDEAALLAML
                     SSA"
BASE COUNT          595 a         1626 c         1582 g          740 t
ORIGIN      
        1 ggtgccgctg gcgcgcaccg ccttcagcgc ggggtgttcc ttgcctgact gcttgcttgc
       61 caccgtcgct cctcgaggtt gcccgggggt agaggataag ccgcggcgcg aatttcgccg
      121 ctggcgcggc ggcgccgcaa acgtcacgat aatcgggcgc gagcgtggcg ccggccatcg
      181 ccgccacgag aaaggcgtcc accgcccgat gatccatctg ctgaacctgc tggctgcgat
      241 cgcgctgctc gtctggggca cgaacatcgt tcgcaccggc gtgatccgga tgttcggcga
      301 gaagctgcgc agcttcctcg ccagcagcct gagcaaccgc ttcaaggccg tgctcgccgg
      361 cctcgcggtg acgggcctgg tgcagtcgag caccgcgacc gcggtgatca tctcgtcgtt
      421 cgtcggcagc gggctggtcg gcctgacctc ggggctggcg gtgatgctcg gcgccgaggt
      481 cggcaccagc ctgatgacgg tggtgttctc gttcgacctg tcgtggctgt cgccgctgct
      541 gatcttcatc ggcgtggtcc tgctgatcgc gaacgagaag agcaccgccg gccgcttcgg
      601 ccaggtcgcg atcggcttcg ggctgatcac gctcgggctg cagctgatcg tctcggcgac
      661 gcaaccgctg atcgcgtcgg cggccttccg cggcctgctg gtcgcgctgc cgaacgacgt
      721 gttcctcgac atcgtcgtcg gcgcgctgct gacgatggcc acctattcga gcctggcgtt
      781 cgtgctgctg acggccacgc tggccgcctc ggggatcctg ccggtgacga tcgcgctcgg
      841 cctggtgctg ggcgcgaaca tcggcagcgg cgtgctggcg atgctgacca gctccgggcc
      901 gccggtcgtg cggctgcgcc tgccgctggg cagcctgctg ttcaagctgg ccggctgcgt
      961 gatcgcgatc ccgctgctgt cgcagatcca cgtgctgctg caggactgca ttccgacggt
     1021 gcacgcgcag gtgatcgcgt tccacctcgg cttcaacgcg gtgcgcgcgc tgctgttcat
     1081 cgggctgacc gcgccgatgg cgcgtctggt cgagaagctg ctgccggcgc cgccgacgca
     1141 gatcggcccg gcgcggccgc ggcacctcga cccctcggcg ctggccacgc cgtcgctggc
     1201 gatcagctgt gcggcgcgcg aggcgctgca ccaggccgac gtggtcgaga ccatgctggg
     1261 cggcatcctg ccggtgctgc gcgacaacga cctcgtgctc gccgagcagc tgcgcaacct
     1321 ggacgacacg gtcgacgagc tctactcggc gatcaagttc tacctgacgc agatctcgcg
     1381 cgaggcgctg tcggaaggcg agggccggcg cgggaccgac atcgtctcgt tcacgatcaa
     1441 catggagcag atcggcgaca tcatcgagcg cgtgatccag gacatcgagg acaagaagat
     1501 ccgcaagaac cggcgcttct ccgacgccgg catggccgag atcgtgcacc tgcacgagcg
     1561 cctgaccgcg aacctgcgcc tgggcatgag cgtgttcctc gacggccacg tgcgcgacgc
     1621 caagaagctg ctcgaggaga aggcgcgctt tcgcgacctc gagcacgaat acgccgccag
     1681 ccacatcgcc cggctgcagg accacaccgc caacagcatc gagaccagct cgctgcacat
     1741 cgacctgatc agcgagctca agcgcatcaa ctcgcacatc tgctcgatcg cctacccgat
     1801 cctcgagtcg gccggcgcgc tgacgaagac gcgcatccgc agctcgcagc acggcgccct
     1861 gtcgggcccc gaggaacgca gcttctgatg actgcgccgc tggcgctgac ctggcccgtc
     1921 gtgctggccg tgctgggcgg cgcgctgctg catgcgtcgt ggaacgcgct gatcaagtcg
     1981 ggccacgaca aggcgctcga caccgcgctg ctgcacttcc tcggcgcgct gatcgccctg
     2041 cccttcatgc tctgggtcgg cccgccgccg gccgcggcgt ggcctttcat tgccgcctca
     2101 ctggcgatcc acatcggcta ctacgtcgcg ctggtcggtg cctaccagca cggcgaactc
     2161 ggcctgacct acccgatcat gcgcggcttc gcgccgatgc tggtcgcgct ggcgagcggc
     2221 gcattcatcg gcgaggcgcc gacgctggcc gcctggctgg gcatcgccgc cgtcacgctc
     2281 ggcgtcgccc tcgtcggcct ggcgcacccg ggcgaagcac tgcaccaccg caaggcgctg
     2341 ctgttcgcat tcgcgaacgc cgcgatcatc gcgctgtaca ccgtggtcga cgggcgcggc
     2401 gtgcgtgcgg cggccgtcga cgaccacggc gcgctgcgct acgtgctgat gctgttcgtg
     2461 ctcgacggca tcgcctaccc gggcttcgta tggctgcgcc gcagcgcggc cggtcggcgc
     2521 gagatcgtcg cctatgcgcg ccggcgctgg ccggtcgcgg cgctcggcgg cagcgcgtcg
     2581 atcggctcgt acgcgatcgc gctgtgggcg atgacgcgcg cgccggtcgc ctcggtcgcg
     2641 gcgctgcgcg agacctcggt gctgttcgcc gcggtgctcg gcacggtgct gctgaaggag
     2701 cgcttcggcc tgcagcgtgc gttcggcacg ctggtcatcg tcgccggcgt gatggcgctg
     2761 cggtttggct agcgcgaagc ggcgcatggg cgcgcaccga ggacgccggg tgacagacga
     2821 gccaggccgc gttcatgcgc tcgacaacat cgccagcagt gcggcctcgt cgagcaccgc
     2881 gatgccgagc tcgttcgcct tgtcgagctt gctcccggcc tcggcgccgg cgacgacgaa
     2941 gtgcgtcttc ttcgacaccg agccgctgac cttgccgccg gccgcctcga tgcggtcctt
     3001 cgcgtcgtcg cgcgacagcc cgggcagcgt gccggtcagc acgaaggtct tgccggccag
     3061 cggcctgggt gccgcgtcgg cggcgccgtc ggtctccggc cattcgatgc cggcggcgcg
     3121 cagttgttcg acgacctcga cgttgtgcgg ctggtcgaag aaggtgcgca cgctgagcgc
     3181 gacgatcggg ccgacatcgt tcacttcgag cagccgctcg acgctggcgg ccatcagccg
     3241 gtcgatgttg ccgaagtgct ttgccaggtc cttcgcggtc gtctcgccga cgtggcggat
     3301 gcccagcccg tacaggaagc gcgcgaagct cgtgcgcttg ctggcctcga ggccggcaag
     3361 caggttgacc gcgctcttct tgcccatgcg gtcgagtgcg gtcaggttcg cgaggccgag
     3421 cttgtacagg tcgggcagcg tgccgacgat gccggcgtcg acgagctggt cgaccagctt
     3481 gtcgcccagc ccctcgatgt cgagcgcgcg gcgctgcgcg aagtgcagga tcgcctgctt
     3541 gcgctgcgcc gggcagaaca ggccgcccga gcagcgccag tcgacctcgc cctcctcgcg
     3601 cgtgatcgcg ctggcacaga tcgggcactt gccggcgagg cgcttgtaca ggtcgaaggg
     3661 ctcgccgacc tgcgccggcc gccgctcgag cacgacgccg acgacctcgg ggatcacgtc
     3721 gccggcacgg cgcacgatca cggtgtcgcc gatgcgcacg tccttgcggc gggtctcgtc
     3781 ctcgttgtgc agcgtcgcgt tgctgaccgt cgtgccgccg acgaacaccg gctccagctt
     3841 cgccaccggc gtcagcttgc cggtgcggcc gacctggatg tcgatgtcgc gcaccacggt
     3901 cgtctgctcc tgcgccgggt acttgtgggc acaggcccag cgcggctcgc gcgacttgaa
     3961 gccgagcgtg gcctgcagcg cgcgatcgtc gaccttgtag acgacgccgt cgatgtcgaa
     4021 cggcagcgca tcgcgacgcg cggcgatgcg cgcgtggtag tcgatcagcc ccgcggcgcc
     4081 gtgcacgacc gcgctctcgt cgctcacggg caccccgagg cgccgcagcg ccgcgagcag
     4141 ttcgccctgg gtcggcggca gcgcccagcc gtcgacctcg ccgaggccgt aggcgaagaa
     4201 gctcagcggc cgcctcaaag ccttgccggc atcgagctgc cgcaccaccc cggccgccgc
     4261 gttgcgcggg ttgacgaagg tcttctcgcc gcgcgcgccg gactcgatga gcttgcgctg
     4321 gcgctcgttc agcgcctcga aatcgtcgcg gcgcatgaag acctcgccgc gcacctcgat
     4381 caccgcggcc tgcgcggcag cggccccacg caggcgcttg ggcaccgtcg cgatcatgcg
     4441 cacggtgtgg gtcacgtcct cgccggtctc gccgtcgccg cgcgtcgcgg cctgcacgag
     4501 ctcgccgcgc tcgtagcgca ggttgatcgc cagaccatcg aac
//
LOCUS       DATMJB010000323         4445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_292154, whole genome shotgun sequence.
ACCESSION   DATMJB010000323 DATMJB010000000
VERSION     DATMJB010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292154"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4055
                     /locus_tag="VNU48_07015"
     CDS             <1..4055
                     /locus_tag="VNU48_07015"
                     /inference="COORDINATES: protein motif:HMM:NF018497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HWI11064.1"
                     /translation="AEKVRQVKEQATALTGAAVAQNASGGLSDDGLKKQLADIRAVEK
                     ATLERIGVEARGDAERRRVADEEQVSATLRAASAQKIIDAQNGLSATYRTQLAQLREL
                     RDAGALTPAQFKQAGAALVAKQPVSERRSSASDDLAKKIRDEADAVKAANDKVIASNQ
                     AFIESVNRRADAIGKTPSQLLAQEAALRGLTAQTAPAIAKLQDLEAKTGQLGKGAFAT
                     RNQLLTLQYTLSDVVASAASGISPLTILLQQGGQVAQIEGGVGGVFRTLLSLVTPARI
                     AVGGFAATVGVLAAAWYEGHKQSKEFNDQVVLSGNYAGKTEGQYNALARSISANTRAS
                     TGTAREFAQALIATGEIGPKVFDLSAEAATRYGEATGKTAKDVATEFAALTRDVTAGA
                     TKMNASMNFLSASQLQQIRNLQDQGKETEAYRIAVDALNERLKGLDANLGTLETTTQS
                     VGKFFREMWDRVLDVGRTETIEQKIAAARGALQNAQRASADAPSIGVYGGPRPSGAAN
                     VETSAENLRLLNRSQTAQESTAAERAQTAEINKKADAASAYYRQAEKGAKAEVGRMRE
                     LAEEERQYQALLNAKAANPNVEVPSAATRAAVQKKIRDSYTDKGAVNDNSAERKAQLD
                     QALKYLQDNLAAQQDALKFNQSSLAADYSAANISLQDYYARRAQITAEGVQREVNQLI
                     DAQALIEVELAKGGFKNAAEKTGFETKLQETIAKRANIERDAANAADLALKDQGAAYK
                     ALQREVLSFQATIDQAQGNDAGAAEKRLQQLVKDAQDFRAKSGGLVSEQDIQRLRFAT
                     QQANALAEAQRQVAAATENAGRAEELYLLLSSKAGVSLESTEQGVYEIRARLVEQLRV
                     AAERTQALAEANAAAAREAGGVADPKLLAAAGEAALAYARALETVDPALNRIREKTDA
                     LAQSIAQSAVGSLTNFGDLYRSRVEKAQQETADQRKAYDAQLSQLRQYLANSNDARER
                     ARLQDLIKQAEARRKSLEVDKGDQYLKTLERDVVGPILKKIKDAAIQQFITEPLEKYL
                     KDSLRQLTGADGFLSKIFGSAGKSTEQQQQTVAITASTNALTIFTNALASSAAAISGA
                     GLGSAYPSGDPAAIKLAGAGIDQSGEDYDHEYTRAQTRATEQAQKLAGSFGQATEMSR
                     QNSAVLGGASSLLEQLGGRAGQAGQALGLLPRIIQLIQAAGTGSAAGGGGWWDALMGF
                     FGGGSTSGGGTGFYSGAATNASTVGMSLAVGTNYVPYDNFKANLHKGEAVVPAAYNPA
                     AGGMVAPQRAGAWGGGSGGGGNTYVTVEDHAGGKVTTEEQQSGQDKLVKIIIEKAVAE
                     VDKRIAKGGSTSEALKAKGVKMGGGNPRRD"
     gene            4057..>4445
                     /locus_tag="VNU48_07020"
     CDS             4057..>4445
                     /locus_tag="VNU48_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11065.1"
                     /translation="MSAALPEILLPYGMPVFQWMGHSIALQSIHANVKFRTGPSLTRP
                     IARSRPRKVPVGLFLTREQMAVFDDWFEDDLDVGTRKFSARVANQGPGLLWWAAEWFD
                     VPVYTPLASGYWRVTGTLLLTGEGSVTG"
BASE COUNT          875 a         1473 c         1533 g          564 t
ORIGIN      
        1 tggccgagaa ggtcaggcag gtcaaggagc aggcgaccgc gctgacaggt gcggctgttg
       61 cgcagaacgc cagcggcggg ctcagcgacg acggactgaa gaagcaactt gccgacatcc
      121 gcgccgtcga gaaggcgacg ctggagcgca tcggtgtcga ggctcgtggc gacgccgagc
      181 gccgccgcgt ggccgatgaa gagcaggttt cagccaccct gcgcgcggcg tccgcgcaga
      241 agatcatcga cgcacaaaac ggcctgtccg ccacgtatcg cacgcaactc gcgcaactgc
      301 gcgaattgcg agacgccggc gcactgacac cggcgcagtt caaacaggca ggcgcagcgc
      361 ttgtcgccaa gcagccagtt tctgagcgcc gctcaagtgc ttctgacgat ctggcaaaaa
      421 agatccggga cgaagccgat gccgtcaagg cggcgaacga caaggtcatc gccagcaacc
      481 aggcattcat cgagtcggtg aaccggcgcg cggatgcgat cggcaagacg ccttctcagc
      541 tgctggccca agaggctgcg ctgcgaggcc tcacggcgca gaccgcgccg gccatcgcca
      601 agctgcagga tctggaagcc aagaccgggc agctcggcaa gggcgcgttc gccacgcgca
      661 accaactgct caccctgcag tacacgctgt ccgacgtcgt ggcgtcggcc gcgtcgggca
      721 tctcgccgct gacgatcttg cttcaacagg gcggccaagt cgcgcagatt gagggcggcg
      781 tgggcggcgt cttccgcacg ctgctgtcgc tggtcacgcc ggcgcgcatc gccgtcggcg
      841 gattcgccgc gacggtcggc gtgctcgccg ctgcttggta cgagggccac aagcagagca
      901 aggaattcaa cgaccaagtg gtgctcagcg gcaactacgc cggcaagacc gagggccagt
      961 acaacgcgct ggctcgcagc atctcggcaa acacgcgcgc gagcacgggt acggcgcgcg
     1021 agttcgcgca ggcgctgatc gccaccggtg agatcggccc gaaggtcttc gatctgtccg
     1081 ccgaggcggc gacgcgctac ggcgaagcca cgggcaagac ggcgaaggac gtcgccacag
     1141 agttcgcggc gctgacgcgc gacgttacgg ccggcgcgac gaagatgaac gcgtcgatga
     1201 atttcctgtc ggcgtcgcag ttgcagcaga tccgcaactt gcaagaccaa ggcaaggaaa
     1261 ccgaggcgta tcgcatcgcg gtcgacgcgc tcaacgagcg tctgaagggc ctcgacgcaa
     1321 atctgggcac gctggagacg acgacgcagt ccgtcggcaa gttcttcagg gaaatgtggg
     1381 accgcgtgct cgacgtcggc cgtaccgaga cgatcgagca gaagatcgcc gccgcacgcg
     1441 gcgcgctgca gaacgcgcag cgcgcatcag ccgatgcgcc gagcatcggc gtctacggcg
     1501 ggccgcgacc gagtggcgcc gccaacgtcg aaacgtcggc agagaaccta cgcctgctga
     1561 accgctcgca gactgcgcaa gagtccaccg ccgccgagcg cgcgcagacc gcggagatca
     1621 acaagaaggc cgacgccgcc tcggcctact accggcaggc cgagaagggc gccaaagccg
     1681 aggtcggcag gatgcgcgag ctcgccgagg aggagcggca gtatcaggcg ctgctcaatg
     1741 cgaaggctgc caatcccaac gtcgaggtgc cgagcgccgc cacgcgcgcc gccgtgcaga
     1801 agaagatccg cgacagctac accgacaagg gcgccgtcaa cgacaactcc gccgagcgca
     1861 aggcccaact cgatcaagcg ctgaagtacc tacaggacaa cctcgccgcg cagcaggatg
     1921 ccctcaagtt caatcagtcg tcactggccg ccgactattc ggcggcgaac atctcgttgc
     1981 aggactacta cgcgcgccgg gctcagatca cggccgaagg cgtgcagcgc gaggtcaacc
     2041 agttgatcga cgcacaggcg ctgatcgagg tcgagctcgc gaagggcggg ttcaagaacg
     2101 ccgccgagaa gaccggcttc gaaaccaaac tgcaggagac gatcgccaag cgcgcgaaca
     2161 tcgaacgcga cgccgccaac gcggccgatc tcgcgctgaa ggaccagggc gccgcctaca
     2221 aagcgctgca gcgcgaggtc ctgagtttcc aggcgacgat cgaccaagcg cagggcaacg
     2281 acgccggcgc ggccgagaag cggctgcagc aactcgtcaa ggacgcgcag gacttccgcg
     2341 ccaagtcggg cggcttggtg tcggagcagg acatccagcg cttgcgcttc gccacacagc
     2401 aggccaacgc gctcgccgag gctcagcgac aggtcgccgc ggccaccgag aacgccggcc
     2461 gcgccgaaga gctctacctg ctgctctcgt cgaaggcggg cgtctcgctc gaatcgacgg
     2521 agcagggcgt ctacgagatc cgcgcgcgcc tcgtcgagca gctccgcgtc gccgccgagc
     2581 gcacgcaggc gctggccgag gcgaacgccg cggcggcacg cgaagccggc ggcgtcgccg
     2641 acccgaaact gcttgcagcc gccggcgagg ccgcgctggc gtacgcgagg gcgctggaaa
     2701 ccgtcgaccc ggcgttgaac cggatccgcg agaagaccga cgcgctggcg cagtcgatcg
     2761 cgcagtcggc cgtcggcagc ctgaccaact tcggcgacct gtaccgctcc cgcgtcgaga
     2821 aggcgcagca ggagacggcg gaccagcgca aggcctacga cgcccaactc agccagctgc
     2881 gccagtacct tgcgaattcg aacgacgccc gcgagcgcgc gcggctgcag gacctgatca
     2941 agcaggccga ggcgcggcgc aagagtttgg aagtcgacaa gggcgaccag tacctgaaga
     3001 cgctcgagcg cgacgtggtc ggcccgatcc tgaagaagat caaggacgcc gcgatccagc
     3061 agttcatcac cgagccgctg gagaagtacc tcaaggactc gctgcgccaa ctcactggcg
     3121 ccgacggctt cctgtcgaag atcttcggca gcgccggcaa gtccaccgag cagcaacagc
     3181 agaccgtggc catcacggcg tctaccaacg cgctcaccat cttcacgaac gcgctggcga
     3241 gcagcgcggc agcgatcagc ggcgccggtc tcggcagcgc ctaccctagc ggcgacccgg
     3301 cggcgatcaa actggcgggc gccggcatcg atcagtccgg cgaggactac gaccacgagt
     3361 acacgcgcgc gcagacacgg gcgacggagc aggcgcagaa acttgcggga agtttcggcc
     3421 aggccaccga gatgtcgagg cagaactctg cggtgctggg cggcgcgagt tcgttgctgg
     3481 agcaactcgg tggcagggcg ggccaagccg ggcaagccct cggcctgctg ccgcggatca
     3541 tccagttgat tcaggcggcc ggcaccggtt cggcggcagg tggcggtggc tggtgggatg
     3601 ccctcatggg gttcttcggc ggcggttcaa cctccggcgg cggtacgggc ttctacagcg
     3661 gcgcggcgac gaacgcgagc accgtcggca tgtcgctggc cgtcggcacg aactacgtgc
     3721 cctacgacaa cttcaaggcc aacctgcaca agggcgaagc cgtcgttcct gccgcttaca
     3781 accctgcggc tggcggcatg gtggcgccgc agcgcgcagg tgcgtggggc ggcggatctg
     3841 gtggcggtgg taacacctac gtgacagtag aggatcacgc cggcggcaag gtgaccaccg
     3901 aggagcagca gtccggccag gacaagctcg tcaagatcat tatcgagaag gccgtcgccg
     3961 aggtcgacaa gcgcatcgcg aagggcggca gcacgtcgga ggcgctgaag gccaagggcg
     4021 tgaagatggg cggcggcaac ccgcggcgag attgacgtga gcgccgcgct ccccgagatc
     4081 ctgctgccgt acggcatgcc ggtgttccag tggatgggcc actcgatcgc gttgcagtcg
     4141 atccacgcca acgtgaagtt ccgcaccggg ccgagcctca cgcggccgat cgcacgcagc
     4201 cggccgcgca aggtgcccgt cgggctgttc ctgacgcgcg agcagatggc ggtcttcgac
     4261 gactggttcg aagacgacct cgacgtcggc acacgcaagt tcagcgcgcg cgtcgcgaat
     4321 caaggcccgg gcctgctgtg gtgggccgcc gagtggttcg acgttcccgt ctacacaccg
     4381 ctggcgtctg gctactggcg cgtgaccggc acgttgctgc tgacaggcga aggcagtgtc
     4441 accgg
//
LOCUS       DATMJB010000324         2708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_264002, whole genome shotgun sequence.
ACCESSION   DATMJB010000324 DATMJB010000000
VERSION     DATMJB010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264002"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..527
                     /locus_tag="VNU48_07025"
     CDS             150..527
                     /locus_tag="VNU48_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11066.1"
                     /translation="MRSAAQLIVAAKAKLGDHRMSDQKFGDQTGYAQQTIAKAKRGGM
                     SDQVAMKIAEILQIEAGEVIWVARVEREQDPTVRRHLEAWVRHVGKALASVPSKAAAF
                     VAAVALGMFSPAHDAQAFGGAGR"
     gene            complement(769..1371)
                     /locus_tag="VNU48_07030"
     CDS             complement(769..1371)
                     /locus_tag="VNU48_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWI11067.1"
                     /translation="MLIGYARVSKLEQDNELQLRALRKVGVRKIYEEKRSAVKHREQL
                     PQLLDQLRAGDLVVVYKLDRLARSVRDLLNIVDRIESAGATFRSLTESFDTTTPAGKM
                     AFQMLGAVAEFERNIIRERSLAGVEVARSRGVRMGRLPALDGRQSAQLSRQWATGKYT
                     KTELASMHGVSLSTVKRTLWRMGLDTISEAHAHGMLPPTP"
     gene            complement(1603..1812)
                     /locus_tag="VNU48_07035"
     CDS             complement(1603..1812)
                     /locus_tag="VNU48_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11068.1"
                     /translation="MMTHTHTHPKQPAATAIATTEAAAPAPDSLAVALDAMRGHPHIA
                     PEVVAACDLIAKRLKVIEDLANIKP"
     gene            complement(1866..2030)
                     /locus_tag="VNU48_07040"
     CDS             complement(1866..2030)
                     /locus_tag="VNU48_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11069.1"
                     /translation="MKTPAFLPAPAAIGREAIVVIAGALLAAFVIGQLPGVRDWIKAQ
                     WGVAKAPDPV"
     gene            complement(2046..2390)
                     /locus_tag="VNU48_07045"
     CDS             complement(2046..2390)
                     /locus_tag="VNU48_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11070.1"
                     /translation="MPDNSTYTAIGAALGVGLAGAAAAVRQIFPNKTKERNEKAERDM
                     ISTLEHERDEARLEAQQERKQRTLDVRMIATLSAENVYLQRYLRKIVRTLPSEARQVV
                     ETDFTPLDELKP"
     gene            complement(2400..>2708)
                     /locus_tag="VNU48_07050"
     CDS             complement(2400..>2708)
                     /locus_tag="VNU48_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017776700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3TM-type holin"
                     /protein_id="HWI11071.1"
                     /translation="AQRQGDMDSIKVSMSAILAEANSADPWTSRARPSFLYVVYILLL
                     FGLPMGLLSAFWPDQATQIALGFKAWLNAIPDSLYLLFGTVMTGYGISRTVEKVKGVA
                     "
BASE COUNT          423 a          847 c          937 g          501 t
ORIGIN      
        1 cgccaggcat cgcgtcgaag cgagtgacgc ggtccgcgct gtagcggtga ggggctatcg
       61 gcatggttac tccttgttta ggggtgacta tactccctaa tcaggcgtaa actctcggct
      121 gtaggctttc cctacaaagg agtcctcaag tgagaagtgc cgcacagctc atcgtggccg
      181 ccaaagcaaa gcttggcgat catcgaatga gcgatcagaa atttggcgat caaaccggct
      241 acgcgcagca gaccatcgct aaggcgaagc gcggtgggat gtcagatcag gtagcgatga
      301 agatcgccga gatcttgcag atcgaagcgg gtgaggtgat ttgggttgcg cgcgtggagc
      361 gcgaacaaga cccaaccgtg cgcaggcacc ttgaggcatg ggtgcgccac gtgggaaaag
      421 ccctggcctc agtgccctcg aaggccgccg cgttcgtcgc ggcggtggcg ctggggatgt
      481 tctcgccggc gcatgacgcg caagcatttg gcggagccgg acggtaatga ccccctggcc
      541 cgcgctctcg actcaaggcc ggcccgcgct gttcccggtg cacgctccct cggccaatga
      601 tatgcccagg gcgccggtcg cctccacgga acccggagca tggacgtgcg cgctgtgcgg
      661 cttccgcggc ttctggtccg gtggcccgca ctgccacggg cgaataagat aaacgcggcg
      721 cgcggcgtcg cacgggggcc ggcttctttg tgggagtcgg cccttttttc atggcgtcgg
      781 cggaagcatc ccgtgcgcgt gcgcctcgct gatggtgtcc aggcccatcc gccagagcgt
      841 ccgcttgacg gtgctcaggc tcacgccgtg catgctggcc aactcggtct tggtgtactt
      901 gccggtagcc cactgtcggg acagctgcgc cgactggcga ccatcgagcg ccggcagccg
      961 ccccatgcga acgcctcgcg atctcgcgac ctcgacgccg gcaagcgacc gctcgcggat
     1021 gatgttgcgc tcgaactcgg cgacggcgcc gagcatctga aaagccatct tgcccgccgg
     1081 cgtggtcgtg tcgaatgact ccgtgaggct gcgaaaggtc gcgccggccg actcgatgcg
     1141 gtcgacgatg ttcagcaggt cgcgcaccga cctggccagc cggtcgagct tgtagaccac
     1201 caccaggtcg ccggcgcgca actggtcgag cagctgcggc agctgctcgc ggtgcttcac
     1261 cgcggagcgc ttctcttcgt agatcttccg aacgcccacc ttgcgcagcg cgcgcagctg
     1321 cagctcgttg tcctgttcca gcttcgagac gcgcgcgtag ccgatcaaca tggcgggacc
     1381 ccgtgcagtg atggggcatt gtgaacgccg atgtgaccct aggtgaatcg ctcaaattgc
     1441 ggagaggccg caagcgtggg gttctgacca ggcgggcgcg catcagcgct tgaaacgccc
     1501 cttcgcgtcg cgcgcgaccg acttgacgcg ccggcctggc acggtgaagc ccctgcactt
     1561 cttgcaaggc gcgaccttgc gctttctctt ggtggccacg ggtcagggct tgatgttggc
     1621 caggtcttca atgaccttga ggcgcttggc gatcaggtcg cacgcggcga cgacctcggg
     1681 ggcgatgtgc ggatgcccgc gcatcgcgtc gagcgccaca gccagcgaat ccggcgccgg
     1741 cgccgccgct tcggtggtgg cgatggcggt tgctgcgggt tgcttcgggt gagtgtgcgt
     1801 gtgcgtcatc atggcttctc tcttggttgt ggccagcgtc ggccgggggt gttgtggggc
     1861 gggggtcaca ccgggtcggg cgccttggcg acgccccatt gcgctttgat ccagtcgcgc
     1921 acgcctggca gctggccgat gacaaatgca gcgagcagcg cgcccgcgat gacgacgatg
     1981 gcttcgcgac cgatggccgc gggcgcggga aggaatgcgg gcgttttcat gggtgatggg
     2041 ccgtcctatg gcttgagttc gtccagcggc gtgaagtcgg tttcgacgac ctggcgcgcc
     2101 tcggatggca gggtgcgaac gatcttgcgc aggtagcgct gcaggtagac gttctccgcc
     2161 gaaagcgtcg cgatcatgcg cacgtcgagc gtgcgctgct tgcgctcttg ctgcgcttcc
     2221 aggcgcgctt cgtcgcgctc gtgctcgagc gtcgagatca tgtcgcgctc ggccttctcg
     2281 ttgcgttctt tcgtcttgtt cgggaagatt tgccgcaccg ctgcagctgc gccggccagg
     2341 cccacgccga gcgcggcgcc gatcgcggta taggtgctgt tgtccggcat ggtccggccc
     2401 tacgccacgc ccttgacctt ctcgacggtg cgcgagattc cgtagccggt catgacggtg
     2461 ccgaagagga gatacagcga atcgggaatg gcgttgagcc aggccttgaa gccgagcgct
     2521 atctgcgtcg cctggtcagg ccagaaggcc gacagcaggc ccatgggcag gccgaacagc
     2581 agcaggatgt agacgacgta cagaaacgac ggccgcgccc gcgaggtcca cgggtcggcc
     2641 gagttcgctt cggccaggat cgccgacatg gacaccttga tgctgtccat gtcgccttga
     2701 cgctgcgc
//
LOCUS       DATMJB010000325         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_798279, whole genome shotgun sequence.
ACCESSION   DATMJB010000325 DATMJB010000000
VERSION     DATMJB010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798279"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VNU48_07055"
     CDS             complement(<1..1169)
                     /locus_tag="VNU48_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWI11072.1"
                     /translation="MDALILARMQFAANITFHILFPTISIALGWLLLFFRLRWLKARH
                     GADQAAAQGWLDAYRFWTKVFALSFALGVVSGITMSFQFGTNWPGFMEHAGNLAGPLL
                     GFEVLTAFFLEAGFLGVMLFGHGKVSERVHLMATFFVAFGTTLSAFWILALNSWMQTP
                     AGYEIVDGVYHVKSWIEVVFNPSFPYRFTHMVLASALTCAFLIAGLSAWQLLRGVGQR
                     SAPRVLRVGLTLAALLIPVQIVVGDAHGLNTLEHQPAKIAAMEGVWDTERGAPLLLFA
                     IPDPVSRSNHFEIAIPRVASLILTHDLDGEVKGMNSFGDAHPPPLPLFFAFRVMVGMG
                     VLMLATSWFGWWCYRRARWQPAALPRALLWLFALMTFAGWVATVAGWYVTEIGRQP"
     gene            1456..>2075
                     /locus_tag="VNU48_07060"
     CDS             1456..>2075
                     /locus_tag="VNU48_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HWI11073.1"
                     /translation="MIHYRIEIANARAHLFRVTLTVARPAPQQRLSLPAWIPGSYLVR
                     EFARHLSGLEATQAGKPVSLQQLDKATWRAGCDDGAELVASYLVYAFDTSVRAAFLDT
                     RRGFFNGSSLCLRVEGREAEPHRLELRKLPAGWQVATALRAIKVDAAGRGVFEATDYD
                     ELVDHPVELGRFWRGSFTAAGVAHELVVAGAFPDFQGETLLADTKR"
BASE COUNT          340 a          734 c          714 g          287 t
ORIGIN      
        1 ggctggcggc cgatctcggt gacgtaccag ccggcgacgg tggcgaccca gccggcgaag
       61 gtcatcaagg cgaacagcca cagcagcgcg cgcggcagcg cggccggctg ccaccttgcg
      121 cgtcggtagc accaccagcc gaaccagctg gtggccagca tcagcacgcc catgccgacc
      181 atgacgcgga acgcgaagaa cagcggcagc ggcggcgggt gcgcgtcgcc gaatgaattc
      241 atgcccttga cctcgccgtc gaggtcgtgg gtgaggatca ggctcgcgac cctcgggatc
      301 gcgatctcga agtggttgct gcggctgacc gggtcgggaa tcgcgaacag cagcagtggc
      361 gcgccgcgtt cggtgtccca cacgccctcc atcgcggcga tcttggcggg ctggtgctcg
      421 agcgtgttca ggccgtgtgc gtcgccgacg acgatctgca ccgggatcag cagcgccgcc
      481 agcgtgaggc caacgcgcag cacgcgcggc gccgagcgct ggccgacgcc gcgcagcagc
      541 tgccaggccg acagcccggc gatcaggaac gcgcaggtca gcgccgaggc cagcaccatg
      601 tgcgtgaagc ggtacgggaa cgaggggttg aagacaacct cgatccaact cttgacgtgg
      661 tagacgccgt cgacgatctc gtagcccgcg ggcgtttgca tccacgagtt cagcgccagg
      721 atccagaagg cgctcagggt cgtgccgaag gccacgaaga aggtcgccat caggtgcacg
      781 cgctcgctga ccttgccgtg gccgaacagc atcaccccga ggaagccggc ctcgaggaag
      841 aacgcggtga gcacctcgaa gcccagcagt ggcccggcca ggttgccggc gtgctccatg
      901 aagccgggcc agttggtgcc gaactggaag ctcatcgtga tgccgctgac cacgccgagc
      961 gcgaagctca gcgcgaacac cttggtccag aagcggtagg cgtcgagcca gccctgtgcg
     1021 gccgcctggt cggcgccgtg gcgcgccttc agccagcgca gccggaagaa cagcagcaac
     1081 cagcccagcg cgatgctgat ggtcgggaac aggatgtgga aggtgatgtt ggccgcaaac
     1141 tgcatgcggg ccaggatcag ggcgtccatg gtcgttgcct cggggtgatc gacgttggcg
     1201 ccgggttcag cgcttcgcat tggcgggcat catcgcccgg ccggtgaatt caagcacttt
     1261 ccgaaccttc gcgcccatct tcaggaactc gttgaaacgg tgggccctgc cgcgacccgg
     1321 cgtcgccgat ggactcgggc gttctgcgcg gcgccgcgcc ggcgtgcagc gacgcgcgac
     1381 aatcgacgcc gacttcgcgc ccgccccgac gggcccgcgc ctgccggccc ctcccaccgc
     1441 tcgcaacccc tgacgatgat ccactaccga atcgagatcg cgaacgcccg cgcccacctg
     1501 ttccgcgtga cgctcacggt ggcccggccc gcgccgcaac agcgcctgag cctgccggcc
     1561 tggataccgg gcagctacct ggtgcgtgaa tttgcgcgcc acctgtcggg cctcgaggcg
     1621 acccaggcgg gcaagcccgt cagcctccag caactcgaca aggccacctg gcgcgccggc
     1681 tgcgacgacg gcgccgaact cgtcgcgagc tacctcgtct acgccttcga cacctcggtg
     1741 cgcgcggcct ttctcgacac ccggcgcggc ttcttcaacg gcagctcgct gtgcctgcgc
     1801 gtcgaggggc gcgaggccga gccgcaccgg ctcgaactgc gcaagctgcc ggcgggctgg
     1861 caggtcgcga ccgcgctgcg cgcgatcaag gtcgacgccg ccgggcgcgg cgtcttcgag
     1921 gcgacggact acgacgagct ggtcgatcac ccggtcgagc tcggccgctt ctggcgcggc
     1981 agcttcaccg ccgccggcgt ggcccacgag ctcgtcgtcg ccggcgcctt tcccgatttc
     2041 cagggcgaga ccctgctcgc cgacacgaag cgcat
//
LOCUS       DATMJB010000326         5965 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_106253, whole genome shotgun sequence.
ACCESSION   DATMJB010000326 DATMJB010000000
VERSION     DATMJB010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106253"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VNU48_07065"
     CDS             complement(<1..1168)
                     /locus_tag="VNU48_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HWI11074.1"
                     /translation="MDLLHNLSLGFGVAFTLQNLLYAFFGAVLGTLIGVLPGLGPVAT
                     IAMLLPSIYALDATPALIMLAGIYYGAQYGGSTTAILINVPGETSSVVTAIDGYAMAR
                     KGRAGSALAAAGLGSFFAGCVGTVIIAAFAPPLTELAFKFGPAEYFSLMVLGLIGAVV
                     LASGSLIKAIAMIILGLLLAQINTDVISGTARYSFDIPELTDGIGFVVIAMGVFGFGE
                     IISNLGQPAEHREVFTKHVKGLWPTRQDFHDAWPAVMRGTALGSLLGVLPGGGALLAS
                     FAAYTVEKKMKQRPGEVPFGKGNIRGVAGPESANNAGAQTSFIPMLTLGIPPNAVMAL
                     MVGAMTIKGIQPGPQVMTSNPELFWGLIASMWIGNLMLVLLNLPLLGLWVKLLTV"
     gene            complement(1173..1637)
                     /locus_tag="VNU48_07070"
     CDS             complement(1173..1637)
                     /locus_tag="VNU48_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HWI11075.1"
                     /translation="MKIKSQRDFVSGLMFVVVGVVFAVGATNYSMGSSAKPGAGYFPL
                     ILSVLMAILGAIVLFKSLTIETEGGDPIGHIAWRPLLVIVVSIAVFGSALPRLGMFLT
                     IPLLIVMVSFAGDEFSWKGVIANSVVLTAGSWVIFIWGLNLTIPLKPWFMGG"
     gene            complement(1735..3519)
                     /locus_tag="VNU48_07075"
     CDS             complement(1735..3519)
                     /locus_tag="VNU48_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWI11076.1"
                     /translation="MKAVTAKPAPAHTGKLDARVLLQWLRDDGLISAEDADQTARRFA
                     GGHSAQHPLVRLASVNLARKGTAKTLDIEALTEWLATRCGLPYLRIDPLRVDVGRVAE
                     IMSITYAERRRALPLTVGLAEVTIATCEPFERSWVPEIEAHVRKTVKLVVASPLELAR
                     YTTEFYTLSRSVRNAIKTGEVSALASFEQLVELGKTTKQLDANDQGVVQVVDWLWQYA
                     FDQRASDIHLEPRRERSVIRFRIDGVMHTVYQLPPGVMNAMIARVKLLGRMDVVEKRR
                     PLDGRIKTRNPSGDEVEMRLSTLPTAFGEKMVMRIFDPDTTVKSIDALGFGPHDGKRW
                     EELVSRPHGIILLTGPTGSGKTTTLYSTLRRLATDEVNVCTIEDPIEMIQPALNQTQV
                     QPSIDLNFAEGLRALMRQDPDIIMVGEIRDLETAEMAIQAALTGHLVFSTLHTNDSAS
                     AVTRLIDLGVPPYLIGSTVIGVLAQRLVRTLCVSCKQPDDGVTRAHLDELVKPWRLSG
                     TVQPHKPVGCIECRMTGFRGRAGLYELLTVTDAARAAIHPVTDASRLRQQALADGLRP
                     LRLAGAMKVAEGLTTIDEVLRATPAWEN"
     gene            complement(3535..4323)
                     /locus_tag="VNU48_07080"
     CDS             complement(3535..4323)
                     /locus_tag="VNU48_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HWI11077.1"
                     /translation="MKRERQSTEPELAPATISEADGVRYLHLGTPWVQGAMRIRRSLA
                     LELEYIQRMMVWMLLRPADELTQGRAVQLGLGAAAITRFCHGTLRMRCTAVELNPSVI
                     DACRLWFHLPADSARFELLQMDAAAFVADPARIETAQALCVDLYDHDAASPVLDSAGF
                     YRDCFALLADGGVMTVNLFGRDASFERSAARIAAAFGHTQVWHLRPTKEGNTVVVALK
                     NVPMPERADLAARADNIERRFKLPASKWLRMIRPLASTATAVEP"
     gene            complement(4320..4817)
                     /locus_tag="VNU48_07085"
     CDS             complement(4320..4817)
                     /locus_tag="VNU48_07085"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HWI11078.1"
                     /translation="MSAVLEGLAPVVDARTRLVVLGSFPGVASLRAQQYYGHPRNHFW
                     PILGALWGIELASAVYAQRLAAARAHGLGLWDVYASCRREGSLDSAIEHAELNDLASL
                     TRRAPALQAIAHNGGESARALKLTRALGLPVHRLPSTSPANASWSFERKLAAWRAVFE
                     QHGVA"
     gene            complement(4888..5691)
                     /locus_tag="VNU48_07090"
     CDS             complement(4888..5691)
                     /locus_tag="VNU48_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWI11079.1"
                     /translation="MWIDSHCHLDAAEFDADRGAVVARARAAGVTRIVIPAVERANFD
                     AVRGLAATHGFAYALGIHPLAVARADDDDLHQLGDAIAEHRDDPRLVAVGEIGLDHFV
                     PGLDRARQERFYAAQLAIARSAGLPVILHVRRSADGLLKQLRLTPVRGGIVHAFNGSA
                     QQAQAFVALGFKLGFGGALTFERALQIRRLAQALPAQAIVLETDAPDIPPQWLYRTAA
                     ERAAGATSRNEPAELPRIARTLAELRGVSLPALAELTSANVRAALPGLA"
BASE COUNT          913 a         2058 c         2116 g          878 t
ORIGIN      
        1 gaacagtcag gagcttgacc cagagcccga gcagcggaag gttgagcagc accagcatca
       61 ggttgccgat ccacatcgag gcgatcaggc cccagaagag ttcggggttg ctggtcatca
      121 cctgcgggcc gggctggatg cccttgatcg tcatcgcgcc caccatcagc gccatcaccg
      181 cgttgggcgg gatgccgagc gtgagcatcg ggatgaacga ggtctgcgcg ccggcgttgt
      241 tggccgactc cggccccgcg acgccgcgga tgttgccctt gccgaacggc acctcgcccg
      301 gccgctgctt catcttcttc tcgaccgtgt aggccgcgaa cgaggccagc agcgcgcccc
      361 cgcccggcag cacgccgagc aaggagccga gcgcggtgcc gcgcatcacg gccggccagg
      421 cgtcgtggaa gtcctgccgg gtcggccaca ggcccttcac gtgcttcgtg aagacctcgc
      481 ggtgctcggc cggctggccg aggttggaga tgatctcgcc gaagccgaac acgcccatcg
      541 cgatcacgac gaagccgatg ccgtcggtca gctcggggat gtcgaagctg tagcgtgcgg
      601 tgcccgagat cacgtcggtg ttgatctgcg ccagcagcag gcccaggatg atcatcgcga
      661 tcgccttgat cagcgagccc gaggccagca cgaccgcacc gatcaggccc agcaccatca
      721 gcgagaagta ctcggccgga ccgaacttga acgcgagctc ggtcagcggc ggcgcgaacg
      781 cggcgatgat caccgtgccc acgcagccgg cgaagaacga gcccagcccg gccgcggcca
      841 gcgccgaacc ggcgcggccc ttgcgggcca tcgcgtagcc gtcgatcgcg gtgacgaccg
      901 agctcgtttc gcccggcacg ttgatcagga tcgcggtggt cgagccgccg tactgcgcgc
      961 cgtagtagat gccggccagc atgatcagcg ccggcgtcgc gtcgagcgcg tagatcgacg
     1021 gcaagagcat cgcgatcgtc gccaccgggc cgaggccggg cagcacgccg atcagcgtgc
     1081 cgagcacggc accgaagaag gcatagagca ggttctgcag cgtgaaggcg acgccgaaac
     1141 caagcgagag gttgtgcagg aggtccatgt gctcagccgc ccatgaacca gggcttcagg
     1201 ggaatcgtca ggttcaggcc ccagatgaag atgacccagg agccggcggt cagcaccacg
     1261 ctgttcgcga tcacgccctt ccagctgaat tcgtcgccgg cgaagctgac catcacgatc
     1321 agcagcggga tcgtcaggaa catgcccagc ctcggcagcg cgctgccgaa caccgcgatc
     1381 gacacgacga tcaccagcag cggccgccag gcgatgtggc cgatgggatc gcccccttcg
     1441 gtctcgatcg tcagcgactt gaacagcacg atcgcaccga ggatcgccat cagcaccgac
     1501 aggatcagcg ggaagtagcc cgcgcccggc ttggccgagc tgcccatcga atagttcgtg
     1561 gcgccgacgg cgaagaccac gccgacgacg acgaacatca ggcccgaaac gaaatcccgc
     1621 tgactcttga tcttcatgcc gtcgcccgca gacttttgtt gcgctggatt ctagacaccg
     1681 ccccatgccc gcaagacgtg gttaatacgt agcggcgtga cgcgaaaccg tgtgtcagtt
     1741 ctcccaggct ggcgtggcgc gcagcacctc gtcgatcgtc gtcaggcctt cggcgacctt
     1801 catcgcgccg gccaggcgca gcggccgcag gccgtcggcg agcgcctgct ggcgcagccg
     1861 cgacgcatcg gtgaccgggt ggatcgccgc gcgcgccgcg tcggtcacgg tcagcagttc
     1921 gtacaggccg gcgcggccgc gaaagccggt catgcggcac tcgatgcagc cgaccggctt
     1981 gtgcggctgc acggtgccgc tcagccgcca gggcttgacg agttcgtcga ggtgggcgcg
     2041 cgtgacgccg tcgtcgggtt gcttgcagct cacgcacagc gtgcgcacca ggcgctgcgc
     2101 gagcacgccg atcacggtcg agccgatcag atagggcggc acgccgaggt cgatcaggcg
     2161 cgtcacggcc gaggccgagt cgttggtgtg caaggtgctg aacaccaggt ggccggtgag
     2221 cgcggcctgg atcgccatct cggcggtctc gaggtcgcgg atctcgccga ccatgatgat
     2281 gtccgggtcc tgacgcatca gcgcgcgcag gccttcggcg aagttcagat cgatcgaggg
     2341 ctggacctgg gtctggttca gcgccggctg gatcatctcg atcgggtcct cgatcgtgca
     2401 gacgttgacc tcgtcggtgg cgagtcgccg caaggtggaa tagagcgtcg tcgtcttgcc
     2461 cgagccggtc gggccggtca gcaggatgat gccgtgcggc cgcgagacga gttcttccca
     2521 gcgcttgccg tcgtgcgggc cgaagccgag cgcgtcgatc gacttgacgg tggtgtcggg
     2581 gtcgaagatg cgcatcacca tcttctcgcc gaacgccgtc ggcagggtcg acagccgcat
     2641 ctcgacctcg tcgcccgacg ggttgcgcgt cttgatgcgg ccgtccagcg gccggcgctt
     2701 ctcgaccacg tccatgcggc cgagcagctt gacgcgcgcg atcatcgcgt tcatcacgcc
     2761 gggcggcagc tggtagacgg tgtgcatcac gccgtcgatg cggaagcgga tcacgctgcg
     2821 ctcgcgccgc ggctcgaggt ggatgtcgga ggcgcgctgg tcgaacgcgt actgccagag
     2881 ccagtcgacg acctgcacca cgccctggtc gttggcatcg agctgcttgg tggtcttgcc
     2941 gagttcgacg agctgctcga agctcgccag cgccgagacc tcgccggtct tgatcgcgtt
     3001 gcgcaccgag cgcgacagcg tgtagaactc ggtcgtgtag cgcgccagct cgagcgggct
     3061 cgcgaccacc agcttcacgg tcttgcgcac atgcgcctcg atctcgggca cccagctgcg
     3121 ctcgaacggc tcgcaggtcg cgatcgtgac ctcggccagg cccaccgtca gcggcagcgc
     3181 gcggcgccgc tcggcgtagg tgatcgacat gatctcggcg acgcgcccga cgtccacgcg
     3241 cagcggatcg atgcgcagat agggcaggcc gcaacgcgtg gccagccact cggtcagcgc
     3301 ctcgatgtcg agcgtcttcg ccgtgccctt gcgcgccagg ttgacgctgg ccagccgcac
     3361 cagcgggtgc tgcgcgctgt ggccgccggc gaagcggcgc gcggtctggt cggcgtcttc
     3421 ggcgctgatc aggccgtcgt cgcgcagcca ttgcagcagc acgcgtgcgt ccagcttgcc
     3481 ggtgtgggcc ggtgcgggtt tggctgtcac agctttcatg cgtcgtcgct cttcctaagg
     3541 ttcgacagcg gtggccgtcg acgccagcgg ccggatcatg cgcagccact tgctggccgg
     3601 cagcttgaac cgacgctcga tgttatccgc gcgcgccgcg aggtcggcgc gctccggcat
     3661 cggcacgttc ttcagcgcga cgaccacggt gttgccctcc ttggtcggcc gcaggtgcca
     3721 gacctgcgtg tggccgaagg ccgcggcgat gcgtgccgcg ctgcgttcga agctcgcgtc
     3781 gcggccgaac aggttcaccg tcatcacgcc gccgtcggcg agcagcgcaa agcaatcgcg
     3841 gtagaacccg gcgctgtcca gcaccgggct ggccgcgtcg tggtcgtaga gatcgacgca
     3901 cagtgcctgc gcggtctcga tgcgcgcggg gtcggccacg aacgcggcgg cgtccatctg
     3961 cagcagctcg aagcgggccg agtctgcggg caggtggaac cagagccggc acgcgtcgat
     4021 cacgctcggg ttcagctcga ccgcggtgca gcgcatgcgc agcgtgccgt ggcaaaagcg
     4081 cgtgatcgcg gccgcgccca ggccgagctg cacggcgcgg ccctgcgtca gctcgtcggc
     4141 gggccgcagc agcatccaca ccatcatgcg ctggatgtac tcgagctcga gcgcgagcga
     4201 ccggcggatg cgcatcgcgc cctgcaccca gggcgtgccc aggtgcaggt agcgcacgcc
     4261 gtcggcctcg gagatggtcg ccggggcgag ttcgggttcg gtgctctgac gctcgcgttt
     4321 catgccactc catgttgttc gaagacggcg cgccaggccg cgagcttgcg ctcgaagctc
     4381 cagctcgcat tcgccgggct ggtcgacggc aggcggtgca ccggcagccc gagcgcgcgc
     4441 gtcaacttga gcgcgcgcgc cgattcgccg ccgttgtgcg cgatcgcctg cagcgccggg
     4501 gcgcgccgcg tcaggctcgc gaggtcgttc agttcggcgt gctcgatcgc gctgtccagg
     4561 ctgccctcgc gccggcaact ggcgtagacg tcccagaggc ccaggccatg ggcccgcgcg
     4621 gcagcgagcc gctgcgcata gacggcgctc gccagctcga tgccccacaa cgcgccgagg
     4681 atcggccaga agtgattgcg cggatggccg tagtactgct gcgcccgcag cgaggccacg
     4741 ccgggaaagc tgccgagcac gaccagccgc gtgcgcgcgt cgacaaccgg ggcgagacct
     4801 tccaggaccg cactcattgg tgcaccctgc ggccctcggc cggtcccgcc gagcgggaat
     4861 gagcccctac gggcggccgg gcgggactca tgcgagaccg ggcaacgccg cccgcacgtt
     4921 cgccgaggtc agttcggcaa gggccggcag cgacacgccg cgcagttcgg cgagcgtgcg
     4981 cgcgatgcgc ggcagttcgg ccggctcgtt gcgcgacgtg gcgccggcgg cgcgctcggc
     5041 ggcggttcgg tagagccact gcggcgggat gtccggggcg tcggtttcca gcacgatcgc
     5101 ctgcgccggc agcgcttgcg cgaggcgccg gatctgcagc gcgcgctcga aggtcagcgc
     5161 gccgccgaag ccgagcttga agccgagcgc gacaaaggcc tgcgcctgct gcgcgctgcc
     5221 gttgaacgcg tgcacgatgc cgccgcgcac gggggtcagg cgcagctgtt tcagcaggcc
     5281 gtcggccgac cggcgtacat gcaggatcac cggcaggccg gcgctgcgcg cgatggcgag
     5341 ctgcgcggca tagaagcgct cctgccgcgc gcggtccagc cccggcacga agtgatccag
     5401 gccgatctcg ccgacggcca cgagccgcgg gtcgtcgcgg tgctcggcga tcgcgtcgcc
     5461 gagctggtgc aggtcatcgt cgtcggcgcg tgccacggcc agtgggtgga tgcccagcgc
     5521 ataagcgaag ccgtgcgtcg ccgcgagacc gcgcacggcg tcgaagttcg cccgctccac
     5581 ggccgggatc acgatgcgcg tcacgccggc ggcgcgggca cgcgcgacga ccgcgccgcg
     5641 gtcggcatcg aactcggcgg cgtcgaggtg gcaatggctg tcgatccaca tggccgcgat
     5701 gatgcaccgg gcgcgacgct gtgccacccg gccgcgcggc gagacaggaa ttggcccgcg
     5761 tgctaccgta gcagcgcggt gtgcaggggc ccgcttcttg catccgcgtc gacacgaccg
     5821 gatcgatgat tttctcggga tcccgacgat gaagaaggca ccgctttaca gccgctctcg
     5881 cccgcccgcc ggggccgtcg tcgccccggc cgccgacgcc gccgcgacca gcgttgccgc
     5941 gccgcaggcg ggcacgcgcc ccgcc
//
LOCUS       DATMJB010000327         8186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_780435, whole genome shotgun sequence.
ACCESSION   DATMJB010000327 DATMJB010000000
VERSION     DATMJB010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8186
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780435"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VNU48_07095"
     CDS             complement(<1..324)
                     /locus_tag="VNU48_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWI11080.1"
                     /translation="MPGPGLRRRRLGLALAAGAASLALPALAQAPGPVRVGYAIARTG
                     PWAAGAQVSQEPNYVLWAQQQNALGGLDVQGRRRPIRLEGFDDHSDIDTCIRLYDRMM
                     NVDKVD"
     gene            617..3433
                     /locus_tag="VNU48_07100"
     CDS             617..3433
                     /locus_tag="VNU48_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWI11081.1"
                     /translation="MDTRNSIRLVSDRLMRLAAAACLLVGLGLSVAAWYHVAGVMRTD
                     GQRSFDRFSAQADAGIEDRARSHLFAISGFHGLFTVSEHVSRAEFHDQFERLRGGRWH
                     PGLLAVQYLERVPEAARTAFEAEVRADTSLVAGGYPDFAIQSIVPRAHYLVTKYSEPP
                     QTGALAIGVDLVAQRDHDDALQRAAESGMPMATAPVRLDESTLGYSVYFAVYARHRPL
                     ETPQQRLAALRGYVRGVFRTASMFDDVLPQEQVDGYRVWIEDLGGATPGDAPAILFDS
                     DPSSSIEQPEPGSGREHTYSAVIGERAWMVHTLRPVVRTATAPWPLTVLLCGVALSLG
                     LWIMLHAGASQYGRATTLARRLSREAEESATQLRNVIDHSADGIVTIDARAALLSANP
                     SAQRMFGRSADELVGRPLVELFDPAARDEMNAWLGQATAAAEPGAGEREEIALRADGS
                     RFPLAMAFNRIDGLQPGLRVGTLRDISERRRIESQMHVMARHDALTGLPNRLLLLERL
                     QQAIAAAGLSGVAPARHVGLMFIDLDRFKTINDSLGHHVGDRVLTEVARRLQAVVRSS
                     DTVARMGGDEFVIVLPQMKAAADAESIAHKVLHALHAPVRVEDHDLAVTVSVGLATWP
                     DMAEDAASLMSRADAAMYSAKRAGRNNWAWFDTVQATAMPHLLQLENDLRHALERGQL
                     ALHFQPQYACAGGALVGAEALLRWHHPKLGMVSPADFIPLAEETGLIVSIGKWVLGEA
                     LRHAVQWQGRDGGALRVAVNLSPRQMDSDVVVQMVADALAESGLPARLLELEITEGSV
                     VRDVERSAMRLSRLAELGVSIAIDDFGVGYSSLSYLRDLPVRKLKIDRSFISPLPGDK
                     GAARMVAALVAMAHELQMEVVAEGVETVEQLALVREFGCETAQGFLLGRPLEAQRFAA
                     LAGTREPARVDENFRPNLVQDCSA"
     gene            complement(3455..3838)
                     /locus_tag="VNU48_07105"
     CDS             complement(3455..3838)
                     /locus_tag="VNU48_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006414039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI11082.1"
                     /translation="MAIELNHTIVHARDPQRSANFLAELLGRPAPVPYGPFLGVALDN
                     GVTLDYIAADAELVIEHYAFLVGEAEFDAIFERIRARALPYWADPRHAQPGRINHHDG
                     GRGVYWNDPDGHYLEIITRPYGSGG"
     gene            3911..5212
                     /locus_tag="VNU48_07110"
     CDS             3911..5212
                     /locus_tag="VNU48_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWI11083.1"
                     /translation="MPALTDDPDTAVTVLPNGVRVVAIRMAQLRTASVSVFVRAGSGH
                     ESAALNGISHVVEHMAFKGTSARDAARINLDAERLGAEVNAHTDKDHTAFHMSGLAAH
                     APRFVAMLGELVRDATFPEAELERERQVLLHEHTEDEDDPMATAYRLFDHACYGLHPL
                     AQAVIGSRRNVERLRRADLTGWVRRHYHAGNVIVAAAGGIDPAAIFDAAQAALGALPS
                     GAASEIAPPVWGGGIRSRRIPGCSQTHIVLGWPLPGLLAAEPAGEVAAALFGEGMSSP
                     LMAELREKRGLVYYAACSADLLAHGGEFVVEASFEADKLDEVLREIACALRAQAEAIT
                     AVDLERARNQLAVRLLRASERPTRRLEDAALDLFALQRVRPQAERVDRVNAVTAAEVR
                     AAFAHMLAAPAAVAIAGRVGRGASERARASLLALGTPVGPD"
     gene            complement(5423..6520)
                     /locus_tag="VNU48_07115"
                     /pseudo
     CDS             complement(5423..6520)
                     /locus_tag="VNU48_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
     gene            complement(6676..7599)
                     /locus_tag="VNU48_07120"
     CDS             complement(6676..7599)
                     /locus_tag="VNU48_07120"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWI11084.1"
                     /translation="MNPAFSVVVFTTIAGAAQGLVVTLALALLAGVPLGAGFTSGALI
                     LAEVMLLIGLGASFGHLGRPERAWRAVLMWRTSWLSRECVVLPAFIGVVGLWWLALRA
                     GIATPLLPLAAIVVAALLWYCTAMIYACLRFIEEWAQPLTPVNFALIGLSSGLVLASA
                     LAACAGEAALLRVAGPCALLVTLLAWATRALALRRNAALKHQSTLQSATGIRAPKLVQ
                     TSMGMSAGAFNTREFFHGRTRALLKQVRLAFLVLGFALPALFALWGVASATALPWALA
                     LACQVPGLLAERWFFFAQARHPQNLYYQVVS"
     gene            complement(7596..>8186)
                     /locus_tag="VNU48_07125"
     CDS             complement(7596..>8186)
                     /locus_tag="VNU48_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010107707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWI11085.1"
                     /translation="NTSGSAGPLADTKPYGADPTGTFFNRVQTYEAGIFPGTDTIHFP
                     KSCLHCEDPPCVPVCPTGASYKRKEDGIVLVDYDKCIGCKYCAWACPYGARELDEERQ
                     VMTKCTLCVDRIYDEHLPPQDRKPACVKACPTGARLFGDVKDPDSEVSKAIRERGGYA
                     LMPEWETQPANQYLPRRVTEVAGGATTVEPKLGGLT"
BASE COUNT         1154 a         2881 c         2952 g         1199 t
ORIGIN      
        1 gtcgaccttg tcgacgttca tcatccggtc gtacaggcgg atgcaggtgt cgatgtcgct
       61 gtgatcgtcg aagccctcga gccggatcgg gcggcggcga ccctgcacgt ccagcccgcc
      121 gagggcgttc tgctgctgcg cccacagcac gtagttcggc tcctggctga cctgcgcgcc
      181 cgcggcccat gggccggtgc gcgcgatggc gtatccgacg cgcaccgggc cgggcgcctg
      241 cgccagcgcg ggcagcgcca ggctggcggc gcccgcggcc agggccaggc cgaggcggcg
      301 tcggcgcaga ccggggccgg gcacggcgcc gggaaggcac gttcggggat tgccgatcat
      361 ctgggacctc cttgcaccgc ttctcgggcc cgcgaggggc gcgtaaaccc tctgtcgcgc
      421 ggcgggcgca tcgggcgatt ggactcctgt tcggcgccga tgtgtgtccg agttggcccc
      481 ttgcgccgcc gcgtcgcccg ggccttgatt tgcatcaacg aagcggctca gtcggcgccg
      541 ccagactccg ataagacctc attgcacgcc gcgcaaggac cgtcaccctg ctcacgggcg
      601 tcgctgatca cacgtcatgg atacccggaa ttcgattcga ttggtcagcg atcggctgat
      661 gcgcctggca gcggcggcct gcctgctggt cggcctcgga ctgagcgtgg ccgcctggta
      721 ccacgtggct ggcgtcatgc ggaccgacgg gcagcgcagc tttgaccggt tcagcgccca
      781 ggcggacgcc ggcatcgagg accgggcgcg cagccacctg ttcgccatct ccggcttcca
      841 cgggctgttc acggtgagcg agcatgtcag ccgcgccgag ttccatgacc agttcgagcg
      901 cctgcgcggc ggccgctggc acccggggct gctggcggtg cagtacctcg agcgggtgcc
      961 ggaggccgcg cgcaccgcgt tcgaagccga ggtgcgcgcc gacacgagcc tggtcgccgg
     1021 cggctatccg gacttcgcga ttcaatcgat cgtcccgcgc gcgcattacc tggtcaccaa
     1081 gtacagcgag ccgccgcaga caggtgcgct ggcgatcggc gtcgatctcg tcgcgcagcg
     1141 cgaccacgac gacgcgctcc agcgcgcggc cgagtccggg atgccgatgg ccacggcacc
     1201 ggttcgcctc gacgagtcga cgctcggcta ctcggtctac ttcgcggtgt acgcgcggca
     1261 tcgcccgctc gagacgccac agcagcgcct cgcggcgctg cgcggctacg tgcgcggcgt
     1321 cttccgcacc gccagcatgt tcgacgacgt gctgccgcag gagcaggtcg acggctaccg
     1381 cgtctggatc gaggacctgg gcggcgcgac gcccggcgac gcgccggcca tcctgttcga
     1441 cagcgacccc tcgagttcga tcgagcagcc cgaacccggc agcggtcgcg agcacaccta
     1501 ttcggcggtg atcggcgagc gggcttggat ggtccacacg ctgcgaccgg ttgtccgcac
     1561 ggcgaccgcg ccgtggccgc tgaccgtgct gctgtgcggc gtcgcgctgt cgctcggctt
     1621 gtggatcatg ctgcacgccg gcgcgagcca gtacggccgc gcgacgacgc tggcacggcg
     1681 cctgagccgc gaggccgagg agagcgcaac gcagctgcgc aacgtcatcg accactcggc
     1741 cgatggcatc gtcaccatcg acgcgcgggc ggcgctgctc agcgccaacc cgagcgcgca
     1801 gcgcatgttc ggccgcagcg ccgacgaact ggtgggtcgc ccgctggtcg aactgttcga
     1861 cccggccgcg cgcgacgaga tgaacgcgtg gctcggccag gccacggcag ccgccgagcc
     1921 cggggccggc gagcgcgaag agatcgcgct gcgcgccgac ggcagccggt tcccgctcgc
     1981 catggccttc aaccgcatcg acgggctgca gcccggtctg cgcgtgggca cgctgcgtga
     2041 catcagcgag cggcgacgca tcgagtcgca gatgcacgtg atggcgcggc acgatgccct
     2101 gaccgggctg ccgaaccggc tgctgctgct cgagcggctg cagcaggcga tcgccgcggc
     2161 cggcctgtcg ggcgtggcgc cggcgcggca cgtggggctg atgttcatcg acctcgaccg
     2221 tttcaagacc atcaacgact cgctcggtca ccacgtcggc gaccgggtgc tgaccgaggt
     2281 cgcgcggcgg ctgcaggccg tggtgcgcag cagcgacacg gtggcgcgca tgggcggtga
     2341 cgagttcgtc atcgtgctgc cgcagatgaa ggccgccgcc gacgccgaat ccatcgcgca
     2401 caaggtgctg cacgcgctgc acgcaccggt gcgggtcgag gaccacgatc tggcggtgac
     2461 ggtcagcgtg ggtctggcca cctggcccga catggccgag gacgcggcca gcctgatgag
     2521 ccgggccgac gccgccatgt acagcgccaa gcgtgccggg cgcaacaact gggcctggtt
     2581 cgacaccgtc caggccaccg cgatgccgca cctgctgcag ctcgagaacg acctgcggca
     2641 cgcgctcgaa cgcggccagc tcgcgctgca cttccagccg caatacgcct gcgcgggcgg
     2701 cgcgctggtc ggcgccgagg cgctgctgcg ctggcaccac cccaagctcg gcatggtgtc
     2761 gccggccgac ttcatcccgc tggccgagga aaccgggctg atcgtgtcga tcggcaagtg
     2821 ggtgctgggc gaggcgctgc ggcacgcggt gcagtggcag gggcgcgatg gcggcgcctt
     2881 gcgcgttgcg gtgaacctgt cgccgcgaca gatggattcc gacgtggtgg tgcagatggt
     2941 ggccgacgcc ctggccgagt ccggcctgcc cgcccgcctg ctcgaactcg agatcaccga
     3001 gggctcggtc gtgcgcgatg tcgagcgctc ggccatgcgg ctgagccggc tggccgagct
     3061 gggggtgtcg atcgcgatcg acgacttcgg tgtcggctac tcgagcctgt cgtacctgcg
     3121 cgacctgccg gttcgcaagc tcaagatcga tcgttccttc atttccccgt tgcccggcga
     3181 caagggcgcc gcccgcatgg tcgccgcgct ggtggcgatg gcccacgagt tgcagatgga
     3241 ggtggtggcc gaaggtgtgg agacggtcga gcagctggcg ctggtgcgcg agttcggctg
     3301 cgaaaccgcg cagggattcc tgctcggacg gccgctcgaa gcgcagcgct tcgccgcact
     3361 ggccggcacg cgcgagccgg cacgcgtcga cgaaaacttc cgcccgaacc tggtgcagga
     3421 ctgcagcgcc tgagcacggc gcgcagcgcg ccactcagcc gccactgccg tagggccgcg
     3481 tgatgatctc gaggtagtga ccgtcggggt cgttccagta gacgccgcgg ccgccgtcgt
     3541 ggtgattgat gcggcccggc tgcgcgtgcc gcgggtcggc ccagtacggc agcgcgcggg
     3601 cgcggatgcg ttcgaagatc gcgtcgaact cggcctcgcc gacgaggaac gcgtagtgct
     3661 cgatcacgag ctcggcgtcg gctgcgatgt agtcgagcgt gacgccgttg tcgagcgcga
     3721 cacccaggaa cgggccgtag ggcaccggcg cgggccgccc gagcagctcg gccaggaagt
     3781 tcgccgagcg ctgcgggtcg cgcgcatgaa cgatggtgtg gttcagttcg atggccatga
     3841 tgcgccctcc gagccggcag tctcgccggc gcctgcgagc actctacgac acacgtacca
     3901 tccggttgcc atgcctgccc tcaccgacga ccccgatacc gccgtcaccg tgctgcccaa
     3961 cggcgtgcgt gtcgtcgcga tccgcatggc gcagctgcgc accgccagcg tcagcgtctt
     4021 cgtgcgcgcc ggcagcggcc acgagagcgc ggcgctgaac ggcatcagcc atgtcgtcga
     4081 gcacatggcc ttcaagggca cgagcgcgcg cgacgcggcc cgcatcaacc tggacgccga
     4141 gcgcctgggg gccgaggtca acgcgcacac cgacaaggat cacaccgcgt tccacatgtc
     4201 cggcctggcc gcgcacgcgc cgcgcttcgt cgcgatgctc ggcgagctgg tgcgcgacgc
     4261 gacctttccc gaggccgagc tcgagcgcga acgccaggtg ctgctgcacg agcacaccga
     4321 ggacgaggac gacccgatgg ccaccgccta ccggctgttc gatcacgcct gctacggcct
     4381 gcacccgctc gcgcaggcgg tgatcggcag ccgccgcaac gtcgagcggc tgcgccgcgc
     4441 cgacctgacc ggctgggtgc ggcgccacta ccacgccggc aacgtgatcg tcgcggccgc
     4501 gggcggcatc gatcccgcgg cgatcttcga tgcggcgcag gccgcactgg gcgcgctgcc
     4561 gagcggcgcg gcgagcgaga tcgcgccgcc ggtgtggggc ggcggcatcc gctcgcgccg
     4621 catcccgggt tgcagccaga cccacatcgt gctgggctgg ccgctgccgg ggctgctggc
     4681 cgccgagccc gccggcgagg tcgccgccgc gctgttcggc gaaggcatga gctcgccgct
     4741 gatggccgag ctgcgcgaga agcgcggcct ggtctactac gccgcctgtt cggccgacct
     4801 gctcgcgcac ggcggcgagt tcgtcgtcga ggcttcgttc gaggccgaca agctcgacga
     4861 ggtgctgcgc gagatcgcct gcgcgctgcg cgcgcaggcc gaagcgatca cggcggtcga
     4921 cctggagcgc gcccgcaacc agctcgcggt gcgcctgctg cgcgcgagcg agcggccgac
     4981 gcgccgcctc gaggacgccg cgctcgacct gttcgcgctg cagcgcgtgc ggccgcaggc
     5041 cgagcgcgtc gatcgcgtga acgcggtgac cgcggccgag gtgcgcgccg cgttcgcaca
     5101 catgctcgcg gcgccggccg cggtggccat cgcgggccgc gtcggccgcg gcgccagcga
     5161 acgggcgcgc gccagtctgc ttgcattggg cacaccggtc ggccccgatt gacggcgcct
     5221 ccggtcgcgg cggcgtcgtt cagcgcgctg cggcagcggc ggcggcttcg gccaggcgct
     5281 tcttctcgcg cagcttctcg agctgctcgt cttcgaggcg gcgccgcgtg tcgagctgct
     5341 tctgcgcctc ctggtggcgc tgttcctcga gctcggcggc cgagggctcc tcgtcgatgg
     5401 ccagggacat gaacgccgac ggcggcggct cgccgcgcgg gctcttcagc ttgatgttca
     5461 ggcgcagctc gttcgccgag tcggcgttgc gcagcgcctc gtcgaagctg atcaggccgt
     5521 cgttgtagag ctcgaacaac gcccagtcga aggtgcgcat gccgagctcg cgcgagcgcg
     5581 acatgatcgc cttgatctcg tggaactcgc cctggaagat gcgctcggcg atggtcggcg
     5641 tgttcagcag gatctcgatc gcggccgcgc ggcccttgcc gtcctcgcgc cgcaccaggc
     5701 gctgcgagac gatcgcgcgc aggttcgccg acaggtccat caacagctgg ttgcgcctct
     5761 cttcggggaa gaagttgatg atgcgctcga tcgtctggct cgcgctgttg gcgtgcaagg
     5821 tgcccaggca caggtgaccg gtctcggcaa aggcgatcgc gtgctccatg gtctcggggt
     5881 cgcggatctc gccgatcagg atcacgtccg gcgcctgccg cagggtgttc ttcagcgcat
     5941 gccgccacga cagcgtgtcg atgccgacct cgcggtgcgt gaccagcgac ttctgcgacg
     6001 ggtgcacgta ctcgaccggg tcctcgacgg tgatgatgtg gccggccgac gagcgattgc
     6061 ggtggtcgat catcgccgcc agcgaagtcg acttgccgga gccggtcgcg ccgaccacca
     6121 gcacgaggcc gcgcttggcc atcacgacct ccttcagcac ctcgggcagg ccgagcttct
     6181 cgaagttcgg gatctccgag gcgatggtgc ggatcaccat gctgacgcag cgctgctgca
     6241 cgtacacgtt gacgcgaaag cgcgagatcc cggggatcga gatcgcgaag ttgcattcca
     6301 tctcgcgctc gaactcctcg cgctgggcct cgttcatcat cgcgtgcgcg agcatccgcg
     6361 cggtctcggc gctgagcttt tgcgttgtca gcggctgcat gctgccgttg gacttcatgc
     6421 tcggcggaaa gtcgcaggcg atgaacaggt cggagccgcc cgcctggctc atcgcggcga
     6481 gcaacttgtg catgtaggca cgggcttgtt cttcggtcag ggcggccatc tcactccatc
     6541 ggggacgagg tgctcgtcgg gcgggtttcg gggtgcgttc ttcgcccgag aatgccagcg
     6601 accgggccgc tgcgctgcgc gcagaagcgg ccgaatccgc ccgttgttcc gccggccgcg
     6661 cggcggccga ttcgatcagg acacgacctg gtaatacagg ttctgcgggt gccgggcctg
     6721 cgcgaagaag aaccagcgct cggccagcag ccccggcacc tggcacgcca gcgccagcgc
     6781 ccagggcagc gccgtggcgc tcgcgacgcc ccacagcgcg aacagcgccg gcagcgcgaa
     6841 gccgagcacg aggaacgcca gcctgacctg cttcagcagc gcccgcgtgc ggccatggaa
     6901 gaactcgcgc gtgttgaacg cgccggccga catgcccatc gaggtctgca ccagcttcgg
     6961 cgcgcggatg ccggtggccg actgcagcgt cgattggtgc ttcagcgcgg cgttgcggcg
     7021 cagcgccagc gcgcgcgtgg cccaggccag cagcgtgacg agcagcgcgc acggcccggc
     7081 cacgcgcagc agcgccgcct cgccggcgca ggccgcgagc gcgctggcca gcaccaagcc
     7141 cgacgacagg ccgatcagcg cgaagttcac cggcgtcagc ggctgcgccc actcctcgat
     7201 gaagcgcagg caggcgtaga tcatcgcggt gcagtaccag agcagcgccg cgacgacgat
     7261 cgcggccagc ggcagcagcg gcgtggcgat gccggcgcgc agcgccagcc accacaggcc
     7321 gaccacaccg atgaacgcgg gcagcacgac acattcgcgc gacagccacg aggtgcgcca
     7381 catcagcacc gcgcgccagg cgcgctcggg ccggccgagg tggccgaacg aggcgccgag
     7441 gccgatgagc agcatcactt cggcgaggat cagcgcaccg ctcgtgaagc ccgcgccgag
     7501 cggcacgccc gcgagcagcg ccagcgccag ggtgacgacc agtccctgcg ccgcgccggc
     7561 gatggtggtg aagacgacga ccgagaacgc cgggttcatg tcaggccgcc gagcttcggc
     7621 tcgaccgtcg tcgcgccgcc cgccacttcg gtgacacgcc gcggcaaata ctggttcgcc
     7681 ggctgcgtct cccactcggg catcagcgcg tagccgccgc gttcgcggat cgccttcgac
     7741 acctcggagt ccggatcctt cacgtcgccg aacaggcgcg cgccggtcgg gcaggccttc
     7801 acgcaggcgg gcttgcggtc ctgcggcggc aggtgctcgt cgtagatgcg gtcgacgcac
     7861 agcgtgcact tggtcatcac ctggcgctcc tcgtcgagct cgcgcgcgcc gtacgggcag
     7921 gcccacgcgc agtacttgca gccgatgcac ttgtcgtagt cgaccagcac gatgccgtcc
     7981 tccttgcgct tgtagctcgc gccggtcggg cacaccggca cgcagggcgg gtcttcgcag
     8041 tgcaggcagc tcttcgggaa gtggatcgtg tcggtgccgg ggaagatgcc ggcctcgtag
     8101 gtctgcaccc ggttgaagaa cgtgccggtc gggtcggcgc cgtagggctt cgtgtcggcc
     8161 agcgggccgg cgctgccgga ggtgtt
//
LOCUS       DATMJB010000328         4908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_421734, whole genome shotgun sequence.
ACCESSION   DATMJB010000328 DATMJB010000000
VERSION     DATMJB010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421734"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VNU48_07130"
     CDS             <1..724
                     /locus_tag="VNU48_07130"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HWI11086.1"
                     /translation="ASALAAAAAPAASAAAPAFEWPVSTRVSYRLTGNYRGEVNGSAQ
                     VEWVRSGSHYQVHVDFIVGPKIAPLITQRSTSDGEITAAGLAPRRYDQDIRFLMSDRP
                     RQTVLFDAGYVVLANGEVRTALPGLQDTASQFVQLTYLFSTRPELLRVGGSVDIPLAL
                     PKKVDVWTYDVVEQEVLNTPLGPLTGLHLKPRGDRKAGALSVEMWFAPQLQYFPARLR
                     FEQDAQTYIDLMISRPPEIANQ"
     gene            766..1554
                     /locus_tag="VNU48_07135"
     CDS             766..1554
                     /locus_tag="VNU48_07135"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HWI11087.1"
                     /translation="MSFETILTERRGEGARQTGLITLHRPKQLNALNDALMDELGAAL
                     KAFDADATIGCIVITGSEKAFAAGADIGAMAKLTLADTYGADYITRNWETIRTIRKPV
                     IAAVAGFALGGGCELAMMCDFIIAADTAKFGQPEIKLGIIPGAGGTQRLPRAVGKAKA
                     MDMVLTGRMMDAAEAERGGLVSRVVPFAELMNVTLAAAEAINELSAMSVMAAKESVNR
                     AFEGTLADGVMFERRLFQSMFATEDQKEGMDAFLNKRKAAFRQR"
     gene            complement(1582..2013)
                     /locus_tag="VNU48_07140"
     CDS             complement(1582..2013)
                     /locus_tag="VNU48_07140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HWI11088.1"
                     /translation="MRALLDVNVWVALFDDAHLFSAQANAFIDQAGVKIATCPLVENG
                     VIRVLNLPSYGRRGAVGLTAVRDRLRQACDELDHEFWPDDVSLRDHTRVDFTRVHGHQ
                     QITDVYLLALAVKHKGCLATFDRAIALSAVAGAQPRHLKML"
     gene            complement(2021..2260)
                     /locus_tag="VNU48_07145"
     CDS             complement(2021..2260)
                     /locus_tag="VNU48_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11089.1"
                     /translation="MRTTLNLAEDALLVAKQIAAREHLSLGDAVSQLVRAGAGALPAM
                     KARALPLRGRFALLPQRDEVVTPEHVRELMDREGI"
     gene            2452..3237
                     /locus_tag="VNU48_07150"
     CDS             2452..3237
                     /locus_tag="VNU48_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11090.1"
                     /translation="MNISTSTSTSAPTSAAATAATTDTGVAATAATTPVPDATDSANE
                     ASGKAHGRRAHGGHGHLRQALNQALQTLGLTGAQGRGDGDDDHGGAGNVKHDMAQFMH
                     ALFQAVKGESPSAASSADPKGGFAAGLSALITQAGSGSAPKALQDAFDRLAADFQPAA
                     APATTGPASTPDAATPTSLPTTAVDANLAAAGTSTTDAPSASASSDASAAPAATTTAA
                     PAATSPALTLQALLSRLQQGMGYDGTRRAWTTPSTGNAVNLTA"
     gene            complement(3282..4790)
                     /gene="lysS"
                     /locus_tag="VNU48_07155"
     CDS             complement(3282..4790)
                     /gene="lysS"
                     /locus_tag="VNU48_07155"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HWI11091.1"
                     /translation="MTDDNQLITERREKLAAIRRQGIAFPNDFKPKHAAAELLRLHGD
                     RSNEELEPLAVKVVVAGRLMLKRVMGKASFGTLQDGSGQIQLFVTRDALGEDAYAAFK
                     HWDLGDILGAEGTLFKTKTGELTVKVDRLRLLTKSLRPLPDKFHGMADQEQKYRQRYV
                     DLITDPDARARFMARSKALASIRQFMVEHHFMEVETPMLHPIPGGANAKPFVTHHNAL
                     DQQMFLRIAPELYLKRLIVGGFERVFEINRSFRNEGVSVRHNPEFTMMEFYAAYWNHH
                     DLMDFTEEVLRHAARVATGSAAITYAGKAVALDQPFARLTVRESLVRHAGLTEAEAVD
                     AAALHAKLKSLGRNSEGAAAGDAWPPAHWTLAELQFGLFEAAVEEQLWQPTFIVDYPI
                     EVSPLARASDTDPSITERFELFITGREYANGFSELNDAEDQAARLKAQVANKDAGDEE
                     AMYYDADFIRALEYGMPPTGGCGIGIDRLIMLITDSPSIRDVILFPSLRKEA"
BASE COUNT          798 a         1689 c         1686 g          735 t
ORIGIN      
        1 cgcaagtgcc ttggccgccg ccgccgcgcc tgctgcgtcc gcggccgcgc cggcgttcga
       61 gtggccggtg tcgacgcgcg tcagctaccg gctcaccggc aactaccgcg gcgaggtcaa
      121 cggcagcgcg caggtcgagt gggtgcgcag cggttcgcac taccaggtgc acgtggactt
      181 catcgtcggc ccgaagatcg cgccgctgat cacgcagcgc tcgaccagcg acggcgagat
      241 caccgccgcg ggcctggcgc cgcgtcgcta cgaccaggac atcaggttcc tgatgagcga
      301 tcgcccgcgc cagaccgtgc tgttcgacgc cggctatgtc gtgctcgcga acggcgaggt
      361 gcgcacggcg ctgcccgggc tgcaggacac cgcgagccag ttcgtgcagc tgacctacct
      421 gttcagcacc cggcctgaac tgctgcgcgt cggcggcagc gtcgacatcc cgctggcgct
      481 gccgaagaag gtcgacgtct ggacctacga cgtggtcgag caggaagtgc tgaacacacc
      541 gcttggcccg ctgaccggcc tgcacctgaa gccgcgcggc gatcgcaagg ccggcgcgct
      601 cagcgtcgag atgtggttcg cgccgcagct gcagtatttc ccggcgcggc tgcgcttcga
      661 gcaggacgca cagacctaca tcgacctgat gatctcgcga ccgcccgaga tcgccaacca
      721 atgagcggcg ctacagtggc gcctccacca acgggaaccg acaccatgag cttcgagacc
      781 atcctgaccg agcggcgcgg cgagggcgcg cgccagaccg gcctgatcac cctgcaccgg
      841 ccgaagcagc tgaacgcgct gaacgacgcg ctgatggacg agctcggcgc cgccctgaag
      901 gccttcgacg ccgacgccac catcggctgc atcgtgatca ccggcagcga gaaggcgttc
      961 gcggccggcg ccgacatcgg cgcgatggcg aagctgacgc tcgccgacac ctatggtgcc
     1021 gactacatca cccgcaactg ggagacgatc cgcacgatcc gcaagccggt gatcgccgcg
     1081 gtcgccggct tcgcactcgg cggcggctgc gagctggcga tgatgtgcga cttcatcatc
     1141 gccgccgaca ccgcgaagtt cggccagccc gagatcaagc tcggcatcat ccccggcgcc
     1201 ggcggcacgc agcggctgcc gcgcgcggtc ggcaaggcca aggcgatgga catggtgctg
     1261 acgggccgga tgatggacgc ggccgaggcc gagcgcggcg gcctggtctc gcgcgtcgtg
     1321 ccgttcgccg agctgatgaa cgtgacgctg gccgccgccg aggcgatcaa cgagctcagc
     1381 gcgatgagcg tgatggcggc gaaggaaagc gtgaaccgcg cgttcgaagg cacgctcgcc
     1441 gacggcgtga tgttcgagcg gcggctgttc cagtcgatgt tcgcgaccga ggaccagaag
     1501 gaggggatgg acgcgttcct gaacaagcgc aaggccgcgt tcaggcagcg ttagctccac
     1561 tgccgaccgg cgctcggccg gtcacaacat cttcagatgc ctcggttgcg cgccggcgac
     1621 cgcggacaac gcgatcgcgc ggtcgaacgt cgcaagacaa cccttgtgtt tgaccgccag
     1681 cgcgagcaga tagacatcgg tgatctgctg gtggccgtgc acgcgcgtga agtcgacccg
     1741 tgtgtgatcg cgcaatgaaa cgtcgtcggg ccagaactcg tggtcgagtt cgtcacaggc
     1801 ctgacgcaat cgatcccgca ccgccgtcag cccgacggcc ccgcggcggc cgtagctcgg
     1861 caggttcaga acccggatca cgccgttctc gaccagcggg caggtggcga tcttcacgcc
     1921 agcctggtcg atgaatgcgt tggcctgcgc ggaaaacaaa tgggcatcgt cgaacagcgc
     1981 gacccagacg ttgacatcga gcagcgcacg catgccgggg tcagatgcct tcgcggtcca
     2041 tcagttcgcg cacatgctcg ggggtgacaa cttcgtcgcg ttgcggtagc agcgcgaagc
     2101 ggcctcgcag cggaagtgct cgagccttca tggcaggaag cgcgcccgcc ccggctctga
     2161 cgagctgcga taccgcatcg cccagcgaca ggtgctcgcg cgcggcaatc tgcttggcga
     2221 cgagcagggc gtcttcggcg aggttgaggg tggtgcgcat ggcatgctcc caatgctgtg
     2281 gctgtccttg cgcaccatag catcaaagtg atgtcaacgc atcattgtag cgcgccggcc
     2341 gacatgtgaa caaatgcaag actgcgtgca gggtggtatc gcagctcaac ccggtgccga
     2401 tgcggccgaa gtcaccggga cgacatccga ttcgttcctg cgagaccgcc catgaacatc
     2461 agcaccagca cctcgaccag cgccccgacc agcgccgcgg ccaccgccgc cacgaccgac
     2521 acgggcgttg ccgcgaccgc ggccaccacg ccggtgccgg acgcgaccga ctccgcgaac
     2581 gaggccagcg gcaaagcgca cggccgccgc gcgcacggcg gccacggcca cttgcgtcag
     2641 gccctgaacc aggccctgca gaccctcggc ctgacgggcg cgcagggtcg aggcgacggc
     2701 gatgacgacc acggcggcgc cggcaacgtc aagcacgaca tggcgcagtt catgcacgcg
     2761 ctgttccagg ccgtgaaagg cgaatcgccg agcgcggcct cgtcggcaga ccccaagggc
     2821 ggcttcgccg cggggctgtc cgcgctgatc acgcaggccg gcagcggcag cgcgccgaag
     2881 gcgctgcagg acgcgttcga caggctggcg gcggacttcc agcccgccgc agccccggcc
     2941 acgaccggcc ccgcgtcgac gcccgacgcg gccacgccga cgagcctgcc gaccaccgcc
     3001 gtcgatgcga acctcgccgc cgccggcacg agcacgacgg acgcgccatc ggcatcggca
     3061 tcatccgacg ccagcgccgc gcccgcggca acgacgacgg cggcgcccgc tgccacgtcg
     3121 ccggcgctga cgctgcaggc gctgctgtcg cgcctgcagc agggcatggg ctacgacggc
     3181 acgcgccgtg cctggacgac cccgtcgacc ggcaacgcgg tcaacctgac cgcctgaggc
     3241 cggggccgct cagccttcgt tgcgccgcga cgaagagccc gtcacgcttc cttcctgagg
     3301 gaaggaaaca ggatcacgtc gcggatgctc ggcgaatcgg tgatcagcat gatcagccgg
     3361 tcgatgccga tgccgcaacc gccggtgggc ggcatgccgt attcgagcgc gcggatgaag
     3421 tcggcgtcgt agtacatcgc ctcctcgtcg cccgcgtcct tgtttgccac ctgcgctttc
     3481 aagcgcgcgg cctgatcctc ggcatcgttc aattcggaga agccgttcgc gtattcgcgg
     3541 ccggtgatga agagctcgaa gcgctcggtg atcgacgggt cggtgtcgga cgcgcgcgcc
     3601 agcggcgaga cctcgatggg gtagtcgacg atgaaggtcg gctgccagag ctgctcttcg
     3661 accgcggcct cgaacaggcc gaactgcagc tcggccagcg tccagtgcgc cggcggccag
     3721 gcgtcgcctg cggcggcgcc ttccgagttt cggcccagtg acttcagctt cgcatgcagc
     3781 gcggccgcgt cgaccgcctc ggcctcggtc agccccgcgt gccgcacgag cgactcgcgc
     3841 accgtcagcc gcgcgaacgg ctggtccagc gcgacggcct tgccggcata ggtgatcgcg
     3901 gccgagccgg tcgccacgcg cgcggcgtgg cgcagcactt cctcggtgaa gtccatcagg
     3961 tcgtggtggt tccagtacgc ggcgtagaac tccatcatcg tgaactcggg gttgtgccgc
     4021 accgacacgc cctcgttgcg gaagctccgg ttgatctcga acacgcgctc gaagccgccg
     4081 acgatcagcc gcttcaggta cagctcgggc gcgatgcgca ggaacatctg ctggtcgagc
     4141 gcgttgtggt gcgtgacgaa gggcttcgcg ttcgcgccgc cggggatcgg gtgcagcatc
     4201 ggcgtttcca cttccatgaa gtggtgctcg accatgaact ggcggatcga cgccagcgcc
     4261 ttgctgcgcg ccatgaagcg cgcccgcgcg tcggggtcgg tgatcaggtc gacatagcgc
     4321 tgacggtact tctgttcctg gtcggccatg ccgtggaact tgtcgggcag cgggcgcagg
     4381 ctcttggtga gcaggcgcag gcgatcgacc ttgacggtca gctcgccggt cttggtcttg
     4441 aagagcgtgc cctcggcgcc gaggatgtcg cccaggtccc agtgcttgaa cgcggcgtag
     4501 gcgtcttcgc ccagcgcgtc gcgggtgacg aagagctgga tctggcccga gccgtcctgc
     4561 agcgtgccga agctggcctt gcccatcacg cgcttgagca tcaggcggcc ggcgaccacc
     4621 accttgaccg cgagcggctc gagttcctcg ttgctccggt cgccgtgcag gcgcaacagt
     4681 tcggccgcgg cgtgcttggg cttgaagtcg ttcgggaagg cgatgccttg ccggcggatg
     4741 gcggcgagct tctcgcggcg ctcggtgatg agctggttgt cgtcggtcat gctggctttc
     4801 gatgagcggg cgattttagt gggagcgttc ggcgccacac gtgtgtccct taaactctcg
     4861 cggatgccga tgcacgccca ccgccccgcc gttaccgatc gaaaggtc
//
LOCUS       DATMJB010000329         3207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_594707, whole genome shotgun sequence.
ACCESSION   DATMJB010000329 DATMJB010000000
VERSION     DATMJB010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594707"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VNU48_07160"
     CDS             complement(<1..530)
                     /locus_tag="VNU48_07160"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HWI11092.1"
                     /translation="MTGTFIDAQPYAWPYNGDLRPANTALVVIDMQTDFCGVGGYVDK
                     MGYDLALTRAPIAPIKTLMNRMRTLGFTIIHTREGHRPDLSDLPANKRWRSRQIGTNG
                     IGIGDAGPCGRILVRGEPGWAIIDELAPLEGEVIIDKPGKGSFYATNLDLILRTQGIT
                     HLILTGITTDVCVHTT"
     gene            complement(542..1459)
                     /locus_tag="VNU48_07165"
     CDS             complement(542..1459)
                     /locus_tag="VNU48_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI11093.1"
                     /translation="MNDVLFPFLAAILGGALRVGVPFLFVSLGECLTEKSGRINLGLE
                     GVLVLSACAGFGGSYLSGSPWVGVLVAAAAGALLALLHGLLCSLPRVNDVATGIALML
                     LGTGLAFYLGKPLIQPQATQLPPLALGAWSGSPLLQAALQVNPLLPLGLLMAVGLYWA
                     FAHTRWGLLVRLAGDSASATKALGYSVSVIRIAATAAGGAIAGLGGASLTLFYPGSWN
                     EGISSGQGLIAVALVIFARWSPLRCVGAALLFGGAGAIGPALQSIGISSGYNLFSAVP
                     YVLTLVILVISCRPGKVAEGSPGELSSAR"
     gene            complement(1467..2558)
                     /locus_tag="VNU48_07170"
     CDS             complement(1467..2558)
                     /locus_tag="VNU48_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI11094.1"
                     /translation="MHKSVADALLPVAALIGALLLFGLFVWLGGVSPVETWVLLFKGA
                     FGDWFSWQNTLTRAAPLMLTALCVALPARAGLIIIGGEGALVLGGLAAAALPYLTPLP
                     GNLIGTTLLCLAGFAAGAAWIALAGALRQYRGVNETISSLLLAYTAIAIFKQFVEGPM
                     RDPATLNKPSTRTLDEAIRIGGILGSDVHWGFVIGVVACLALGAWLAFTARGFSVRVV
                     GGNPRTAQLVGLPASALILLACGLGGGCAGLAGAIEVAAVHTNANASLIAGYGYAGIL
                     VSFIARHSPFAIVPVAILFGGFGAAGSLLQRRLGLPDASVQVLQGIAFVLILASEALR
                     GVDWKAAGERFRRSMQPREVPLSSTVKPT"
     gene            complement(2659..>3207)
                     /locus_tag="VNU48_07175"
     CDS             complement(2659..>3207)
                     /locus_tag="VNU48_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWI11095.1"
                     /translation="RSVDPKITTTVIFTGEWSMPVKEAEATNSLADQGVDVFTMHVDS
                     PKVIVETAAKRGKMVCGYHVSQAKLAPAAYLTGAEWNWSTAYKTIIDAAQAGKPHPNF
                     LRGGLKEGYVKMSPYGAMVSDGAKTMADGIKAKMIAGSFDIFVGGLKSNTGAVVVPKT
                     LKQTDAELEKMNYLVEGVIGSV"
BASE COUNT          512 a         1136 c         1116 g          443 t
ORIGIN      
        1 atggtggtgt ggacgcacac gtccgtcgtg atgcccgtga ggatcaggtg cgtgatgccc
       61 tgcgtgcgca ggatcaggtc cagattggtg gcgtagaacg agcccttgcc gggcttgtcg
      121 atgatcactt cgccttcgag gggggcgagc tcgtcgatga tggcccagcc tggctcgcca
      181 cgcacgagga tgcgcccgca ggggccggcg tcgccgatgc cgatgccgtt cgtgccgatc
      241 tggcgcgagc gccagcgctt gttggccggc aggtccgaca gatcgggccg atgcccttcg
      301 cgggtgtgga tgatggtgaa gcccagcgtg cgcatccgat tcatcagcgt cttgatcggc
      361 gcgatcggcg cgcgcgtcag cgccaggtcg tagcccatct tgtcgacgta gccgccgacg
      421 ccgcagaagt cggtctgcat gtcgatcacg acgagcgcgg tattcgccgg ccgcaggtcg
      481 ccgttgtagg gccaggcgta gggctgggcg tcgatgaagg tgccggtcat catgatcgtg
      541 ttcagcgtgc cgacgacagt tcgcccgggc tgccttcggc gaccttgccg ggccggcagc
      601 tgatcaccag gatcaccagc gtgaggacgt acggcaccgc actgaacagg ttgtagcccg
      661 agctgatgcc gatcgactgc agcgccgggc cgatcgcgcc ggcgccaccg aacagcagcg
      721 ccgcgccgac gcagcgcagc gggctccagc gcgcgaagat caccagcgcc accgcgatca
      781 ggccctggcc gctggagatg ccttcgttcc agctgcccgg gtagaacagc gtcagcgacg
      841 caccgccgag cccggcgatc gcgccgccgg cggcggtggc cgcgatgcgg atgacgctga
      901 ccgaatagcc cagggccttg gtcgcgctcg ccgagtcgcc cgccagccgc accagcaggc
      961 cccagcgcgt gtgcgcgaac gcccaataca ggcccaccgc catcagcagc cccagcggca
     1021 acagcgggtt gacctgcagc gcggcctgca gcagcggcga gccgctccag gcgccgagcg
     1081 cgagcggcgg cagctgtgtg gcctgcggct ggatcagcgg cttgccgagg tagaacgcga
     1141 ggccggtgcc gagcagcatc aacgcgatgc cggtggccac gtcgttgacg cgcggcagcg
     1201 agcacagcag cccgtgcagc agcgccagca acgcgcccgc cgcggcggcg accagcacgc
     1261 cgacccacgg agaaccgctc aggtacgagc cgccgaaccc ggcacacgcc gacagcacca
     1321 gcacgccttc gaggccgagg ttgatgcggc ccgacttctc ggtcaggcat tcgcccaggc
     1381 tgacgaacag gaacggcacg ccgacgcgca gcgcaccgcc gaggatcgcg gcgaggaacg
     1441 ggaacagcac gtcgttcatg gcgcgctcag gtcggcttca cggtgctgct cagcggcacc
     1501 tcgcgcggct gcatcgagcg ccggaagcgt tcgcccgcgg ccttccagtc gaccccgcgc
     1561 agcgcctcgc tggccaggat cagcacgaac gcgatgccct gcagcacctg caccgacgcg
     1621 tcgggcaggc ccaggcgccg ctgcagcagg ctgcccgccg cgccgaagcc gccgaacagg
     1681 atcgcgaccg ggacgatcgc gaacggcgag tgccgcgcga tgaacgagac caggatgccg
     1741 gcgtagccgt aacccgcgat cagcgacgcg ttcgcgttgg tgtgcaccgc cgcgacctcg
     1801 atcgcaccgg ccaggccggc gcagccgcca cccaggccgc acgccagcag gatcagcgcg
     1861 ctcgccggca ggccgacgag ttgcgcggtg cgcgggttgc cgccgaccac gcgcaccgag
     1921 aagccgcgtg cggtgaaggc cagccaagcc ccgagcgcga ggcacgcgac cacgccgatc
     1981 acgaagcccc aatgcacgtc ggagcccagg atgccgccga tgcggatggc ctcatcgagc
     2041 gtgcgcgtcg acggcttgtt cagcgtcgcc gggtcgcgca tcgggccttc gacgaactgc
     2101 ttgaagatcg cgatcgcggt gtaggccagc agcaggctcg agatcgtttc gttgacgccg
     2161 cggtactggc gcagcgcgcc ggccagcgcg atccacgccg cgccggccgc gaagccggcc
     2221 agacacagca gcgtcgtgcc gatcaggttg cccggcagcg gcgtcaggta gggcagcgcg
     2281 gccgcggcca gcccgccgag caccagcgcg ccctcgccgc cgatgatgat cagcccggcg
     2341 cgcgccggca gcgcgacgca cagcgcggtc agcatcagcg gtgcggcgcg cgtcagcgtg
     2401 ttctgccacg agaaccagtc gccgaacgcg cccttgaaca gcagcaccca ggtctcgacc
     2461 gggctcacgc cgccgagcca gacgaacagg ccgaacagca gcagcgcgcc gatcagcgcg
     2521 gcgaccggca gcagtgcgtc agcgactgac ttgtgcatgg cttactcccg cggcctcggt
     2581 ccagcgccgg gccgccccaa gcggaccgag ctccccctcg ggggggcgat gccgcaggca
     2641 tcttgggggt caacatgact agacggagcc gatcacgcct tcgaccaggt agttcatctt
     2701 ctcgagttcg gcgtcggtct gcttcagggt cttgggcacc acgaccgcac cggtgttgct
     2761 cttcaggccg ccgacgaaga tgtcgaagct gccggcgatc atcttcgcct tgatgccgtc
     2821 ggccatcgtc ttcgccccgt cggacaccat cgcgccgtag ggcgacatct tgacgtagcc
     2881 ctctttcagc ccgccgcgca ggaagttcgg gtgcggcttg ccggcctgcg ccgcgtcgat
     2941 gatggtcttg tacgcggtgc tccagttcca ttcggcgccg gtcaggtacg cggcgggcgc
     3001 gagcttggcc tggctgacgt ggtagccgca gaccatcttg ccgcgcttcg cggcggtctc
     3061 gacgatcacc ttgggcgagt cgacgtgcat cgtgaacacg tcgacgccct ggtcggccag
     3121 gctgttcgtc gcctcggctt ccttgaccgg catcgaccac tcgccggtga agatcaccgt
     3181 ggtcgtgatc ttcgggtcga ccgagcg
//
LOCUS       DATMJB010000330         6718 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_351162, whole genome shotgun sequence.
ACCESSION   DATMJB010000330 DATMJB010000000
VERSION     DATMJB010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351162"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1202
                     /locus_tag="VNU48_07180"
     CDS             255..1202
                     /locus_tag="VNU48_07180"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI11096.1"
                     /translation="MTLILRGLALLKFLVLGTGIGARASAQTLPPEVSWAIAAQHEYQ
                     VSPNITYLTADNHEVKLDVYHYRQGGKRPTVINIHGGGWLPGFTKDMYPGAFWPFLAL
                     NWNVVNIDYRHSGVALAPAAVEDCLCALRWVIRNAAEYNIDTDQIVVFGQSAGGHLAL
                     MTGMVPESAGLTTRCPGTEPLKVAAIIDMFGITDVGDLLTGENRQSYAVAWLGAQPDI
                     AEVARRSSPLTYVRPGLPPILIMHGDKDTGVPYTHSVRLRDALTKAKARVQLHTVAGG
                     GHGDWDAAQFDAAYRAMFRFLAAHGITVQPLPAGKQRPS"
     gene            1357..2274
                     /locus_tag="VNU48_07185"
     CDS             1357..2274
                     /locus_tag="VNU48_07185"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWI11097.1"
                     /translation="MYRRMAVTRQIERLMLRHVREQKFSGWWHPGEGQEAAGIGATAA
                     MRTDDYLWYQGRGTSWAIGKGMGPGPILGDLLGKVSGSTRGKGAGVPHWHDRSIGVMG
                     EGATLGSVYPLAAGSALASKIRKDGRVSLADFGDGTAARGTFHETMVHAAAWKLPLIY
                     FCENNQYLVGTRLRDVSPTENIADYAAGYHIPGVIVDGQDAVAVYEAVATAAARARAG
                     DGPTLIEAKVFRRQGHYAGDPQQYRDLDAELKHYRDPLDVLGARVGEARAKLLDAEAE
                     QEVQRAYAAAQAAAEPDESVIYQDLYHGQ"
     gene            2264..3253
                     /locus_tag="VNU48_07190"
     CDS             2264..3253
                     /locus_tag="VNU48_07190"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWI11098.1"
                     /translation="MASDMQNIGYNAAMSQALVEEMRRDDSIFVLGQGVATGGWFSTE
                     KGLAAEFGVDRVLDTNISEAFQAGVVAGAAIAGMKPVINMGFGDFALIAGDEIFHKLA
                     KWRYMHGDSFPMTAVVIFPIGAMGGAGPEHSGCSEVLGMHYPGLKVVVPSTAADAKGL
                     MRAALREPNPVIFHSSQSLGWTKGEVPTDPEFIVPIGKAVVRRAGQSATVVSYGAMMP
                     RCLEAAEQLASEGIDLEVIDLRTLVPLDWPTVLGSVRRTHRGMVVHEATRTAGAGAEI
                     AARMQEEAFFDLEAPILRLGAKDVPLPQHAMLEALCIPSVAQIAAEARRLMSL"
     gene            3280..4551
                     /locus_tag="VNU48_07195"
     CDS             3280..4551
                     /locus_tag="VNU48_07195"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWI11099.1"
                     /translation="MSLLTALRIPHMGSVENAKLVSWRVAEGAAFKAGEPLYEIETDK
                     TVVEVEAEADGVLARRDAAEGDEKKVGEHIGYVAPAGSSRGDFEAALRALDAEAAQPE
                     AVAAPLALQPADATAPDVSARHSPLVRRLAAEHGVDLATLKGSGPGGRIVGDDVLRAA
                     QKAPAAAGSLPVPPGYENVPVEAVPNSSRRRAISRRLLESARNTASLTADMQIDLTAT
                     FQLRAAMNERLRAKGDPAVSLLALIAHALCKVLRLRPNFNASFTDSHTLLWQAVNLGI
                     AIDTRDGLIVPVIKDADKLSPAQIDAAIKSLAARARDNQLRPDELEGGTFTLSNPGSL
                     GPVLRAEAIINPPQVALLGLPAVLRVPVAVDAGDGKFRVEVRQVIRPSLTFDHCALDG
                     GQVIGFLNDLRGLLETAPAFDTLPPETPGAS"
     gene            4548..6143
                     /locus_tag="VNU48_07200"
     CDS             4548..6143
                     /locus_tag="VNU48_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadD3 family acyl-CoA ligase"
                     /protein_id="HWI11100.1"
                     /translation="MSALPAIATIPQLLALSAERHAGHVAIDDAGVRLTYAELHAQVL
                     RAARALIALGIRTGDRVAVWGPNIHEWIVAALAIQTAGGVLVPVSTRMKGLEAADVLQ
                     QSEARVLFCIGDFLGQFFPRLLDGHDLPALQYRIVLRGGAQDRPRTLDWDAFLAHSER
                     TDDATLARRMAEVKPDSLSDVLYTSGTTGRPKGVMTTHAQNLRAFECWTGVLGMTSAD
                     RYLIVNPFFHSAGYKAGWLAALIRGATILPHPVFDAEQILLRIAQERISFLLGPPTLF
                     LTLLAHPRVKEFDLSCLRVAVTGAASVPPVLVRRMRDELGFRIVVTAYGLTECCGFAT
                     ICNPQDDAETIANTCGQAIPDVEVRCADPQGNTVPAGEPGEVLIRGYNVMQGYLNNEA
                     ATREAIDAEGWLHTGDVGVMDDKGYLRITDRMKDMFIVGGFNCYPAEIERLANAHPAI
                     ANIAVIGAADERQGEVGRAFIVLRPGTTLSAEEFVAWCRAHMTNYKVPRYVDILPALP
                     LNGAGKVDKLALRAMPQVSPIGR"
     gene            6168..>6718
                     /locus_tag="VNU48_07205"
     CDS             6168..>6718
                     /locus_tag="VNU48_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011518013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HWI11101.1"
                     /translation="MSHFNPVSDSPITEDDAFIARALEKASVPALMMTLTHITGQSRW
                     LDGAIRPKNPLIGELQGYLSDAEQAQVRAEALQVLKAYRDGGSQMPAPPDDARLLQML
                     NFIVGTEVPDDYVPMIRDELSLGGDDANSVRLERPLAEDEKANYHTLVIGAGLSGLLM
                     GLRLKELGVPFTIVEKNDSVGGT"
BASE COUNT         1147 a         2260 c         2257 g         1054 t
ORIGIN      
        1 ggcaacagtc gataccgcag tatccgcaga agagtcttgc caggatcatc aacggatacc
       61 gggtcgcccg acatgggtgg agctgcctag tccggttgga cgaggcggct gcctggtggg
      121 cgccgtcacg ggtcattctg accagctagt ccctgtggac gatgctgctg ctgacaggca
      181 ctcggcacaa tcgcctgacg ccgaaaaaac gacccaccgg gtccgcggag caggagacaa
      241 aggagcggcc ttgcatgacc ctcatccttc gtggacttgc gctgctgaag ttcctggtac
      301 tcgggaccgg catcggtgct cgggcgagcg cgcagacgct gccgcccgaa gtgtcatggg
      361 cgatcgcggc gcagcatgaa tatcaggtgt cccccaacat cacgtatctg accgcagaca
      421 accatgaggt gaagctcgac gtctatcact atcgccaagg cggcaagcgc ccgacagtga
      481 tcaatatcca tggcggcggc tggctgcccg gcttcaccaa ggacatgtat cccggcgcgt
      541 tctggccgtt cctggcgctg aactggaatg tcgtcaacat cgattaccgg cactccggag
      601 tcgcgctggc gccagcggcg gtagaggact gcctgtgcgc gctgcgctgg gtcatccgca
      661 atgccgccga atacaacatc gacaccgatc agatcgtggt gttcgggcaa tcggccggcg
      721 ggcatctggc gttgatgacc ggcatggtgc cggaatccgc aggcctgacg acgcgatgcc
      781 caggcaccga gccgctcaag gtggcggcca tcatcgacat gttcggcatc accgatgtgg
      841 gcgacctgct gaccggcgag aaccgccaga gctacgcggt tgcctggctc ggcgcacagc
      901 ccgacattgc cgaagtggcg cggcgcagct ctccgttgac ctatgtgcgg ccgggcctgc
      961 cgcccatcct gatcatgcat ggcgacaagg acaccggcgt gccttacacc cacagcgtac
     1021 ggctgcgcga cgcgctgacg aaggcgaagg cacgcgttca actccataca gtcgcaggcg
     1081 gcggccacgg cgactgggat gcagcacagt tcgacgccgc gtaccgcgcg atgttccgct
     1141 tcctggccgc gcacggcatc acggttcagc cgctgcccgc cggcaagcaa cgcccctcct
     1201 agtccctgtg gacgatgtga ccgattcgcc gttcgccaca atcgcttcag ccttccaagg
     1261 tctatcgacc ccgcgaagag caggagacaa aggagcgcta tgacaagaga ccgcaccgag
     1321 gcagccgccg gactgagcac ggtccagttg gagacgatgt accgccgcat ggcggtcacg
     1381 cgccagatcg agcggctgat gctgcgccat gtgcgcgagc agaagttttc cggctggtgg
     1441 caccccggcg aggggcagga agccgcgggc atcggcgcca ccgctgcgat gcgaaccgac
     1501 gactacctgt ggtaccaggg gcgcggcacc tcgtgggcca tcggcaaggg catgggcccc
     1561 gggccgatcc ttggcgactt gctcggcaag gtcagcggct cgaccagggg caagggtgcc
     1621 ggcgtgcccc attggcacga tcgctcgatc ggcgtgatgg gcgagggcgc cacgctgggc
     1681 agcgtctacc cgctcgctgc cggttcggcg ctggcctcga agattcgcaa ggatggccgt
     1741 gtctcgctgg ccgacttcgg agacggcacc gccgcgcgcg gaacctttca cgagacgatg
     1801 gtgcatgccg ccgcctggaa gctgccgctg atctacttct gcgagaacaa ccagtacctc
     1861 gtgggcacgc ggctgcgaga cgtcagtccc accgagaaca ttgccgacta cgcggcgggc
     1921 taccacattc ccggcgtcat cgtcgacggc caggacgcgg tggcggtgta cgaagcggtg
     1981 gccacggccg cggcgcgtgc acgtgccggc gacgggccca cgctcatcga ggccaaggtg
     2041 tttcgtcgcc agggccacta tgccggcgac ccgcagcaat accgtgacct cgacgccgag
     2101 ttgaagcact accgcgatcc attggacgtg ctcggcgcgc gcgtcggcga ggccagggcc
     2161 aagctgctcg acgccgaggc cgagcaggaa gtgcaacgcg cctatgccgc cgcccaggcg
     2221 gccgcagaac ccgacgagtc ggtgatctac caggatctct accatggcca gtgacatgca
     2281 aaacatcggc tacaacgccg cgatgagcca ggcgctcgtc gaggagatgc ggcgcgacga
     2341 cagcatcttc gtgctcggcc agggggtggc cacgggcggc tggttctcga ccgagaaggg
     2401 tctcgcggcc gagttcggcg tcgaccgggt gctcgacacg aacatttccg aagccttcca
     2461 ggccggggtc gttgccggcg ccgccatcgc cggcatgaag ccggtcatca acatgggctt
     2521 cggagacttc gcgctcattg ccggtgacga gatctttcac aagctcgcga agtggcgcta
     2581 catgcacggc gacagctttc cgatgaccgc ggtcgtgatc ttcccgatcg gcgccatggg
     2641 cggcgccggg cccgagcact ccgggtgctc cgaggtgctg ggcatgcact accccggact
     2701 caaggtcgtc gtgccctcga ccgccgccga cgccaagggc ttgatgcgtg ccgcgctgcg
     2761 cgagccgaat cccgtgatct tccattcgtc gcagtcgctc ggctggacca agggtgaggt
     2821 gccgaccgat cccgagttca tcgtgccgat cggcaaggcg gtcgtgaggc gcgccggcca
     2881 atcggcgacc gtcgtcagct acggcgcgat gatgccgcgc tgtctcgaag cggccgaaca
     2941 gctggcgagc gagggcatcg acctcgaggt gatcgacctg cgcacgctcg tgccgctcga
     3001 ctggcccacg gtgctgggct cggttcgccg cacgcaccgg ggcatggtcg tgcacgaggc
     3061 gacgcgcacc gccggggccg gcgccgaaat cgcggcacgc atgcaggagg aagcattctt
     3121 cgatctggag gcgcccatcc tgcgcctggg cgcgaaggac gtgcccttgc cgcaacacgc
     3181 gatgctcgag gccttgtgca tcccgtcggt ggcgcagatc gccgccgaag cccggcgact
     3241 catgtcgctg tgaccgaaac catctgaccg aggacttcga tgagtctgct gaccgcactt
     3301 cgcattcccc acatgggatc ggtagagaac gccaagctcg tgagctggcg ggtggccgag
     3361 ggcgctgcct tcaaggccgg cgagccgctc tatgagatcg agaccgacaa gacggtggtg
     3421 gaagtcgagg ccgaggccga tggcgtgctc gcccgccgcg atgccgccga aggtgacgag
     3481 aagaaggttg gcgaacacat cggctatgtg gcgccggcgg gcagctccag gggtgatttc
     3541 gaggcggccc tgcgcgcgct cgacgccgag gctgcgcaac ccgaggcagt cgccgcaccg
     3601 ctcgcgttgc agccggcgga tgcgacggcg cccgacgttt cggcacggca ctcacccttg
     3661 gtccgccgtc tggcggccga acacggtgtc gacctggcga cgttgaaagg gtccggcccc
     3721 ggcggccgca tcgtcggcga cgatgtgctt cgcgcggcgc agaaggcgcc tgccgcggcc
     3781 ggttcgctgc cggtgccgcc gggctacgaa aatgtcccgg tcgaagccgt gcccaacagt
     3841 tcacgacgtc gcgccatctc gcggcgactg ctcgagtcgg cgcgaaacac cgcgtcgctg
     3901 acggccgaca tgcagatcga tctgaccgcg accttccagt tgcgcgccgc gatgaacgag
     3961 cgtctgcgcg cgaagggcga tcccgccgtg tcgttgctgg cgctgattgc acacgcgctg
     4021 tgcaaggtcc tgcgactgcg accgaacttc aacgccagct tcaccgacag ccacaccctg
     4081 ctttggcagg cggtgaatct gggcatcgcg atcgacaccc gcgacgggct gatcgttccg
     4141 gtgatcaagg atgcggacaa gctcagcccg gcgcagatcg atgcggccat caagtcactc
     4201 gcggcgcggg cgcgcgacaa ccagttgcgg cccgacgaac tcgagggcgg aacgttcacg
     4261 ctgtcgaacc ccggctcgct ggggccggtc ctgcgcgccg aagcgatcat caaccccccc
     4321 caggtggcct tgctcggcct gcccgccgtg ttgcgcgtgc cggtggccgt cgatgccggc
     4381 gatggcaagt tccgcgtcga ggtgcgtcaa gtgatccggc cttcactcac cttcgaccac
     4441 tgtgccctcg atggtggcca ggtgatcggc ttcctcaacg atttgcgcgg actgctggag
     4501 actgcgccgg cgttcgacac gctgcctccc gagacgccgg gggcgtcgtg agcgcacttc
     4561 ccgccatcgc gaccatcccg cagctgttgg cgctcagcgc agagcgccac gccgggcacg
     4621 ttgcgatcga cgacgcaggg gttcggctga cctatgccga actgcatgcg caggtgctgc
     4681 gggcggcgcg ggccctgatc gcgctcggca tccggaccgg cgaccgcgtt gccgtgtggg
     4741 ggcccaacat ccacgagtgg atcgtggccg cgctggcgat ccagacggcg gggggtgtgc
     4801 tcgtgccggt cagcacgcga atgaagggcc tcgaggcggc cgacgtgctg cagcagagcg
     4861 aagcgcgcgt tctgttttgc atcggcgact ttctggggca gttctttccg cgcctgctcg
     4921 acgggcatga cctgccagcg ctgcagtacc ggatcgtcct gcgaggcggg gcgcaagacc
     4981 ggccacgcac gctcgactgg gacgccttcc tcgcccattc cgagcgcacc gacgacgcca
     5041 ccctcgcacg ccgcatggcg gaggtgaagc ccgacagctt gtcggacgtg ctgtacacct
     5101 ccggcacgac cggtcgcccc aagggcgtga tgacgacgca cgcgcagaac ctgcgcgcct
     5161 tcgagtgctg gaccggcgtg ctgggcatga ccagcgccga ccgctacctg atcgtcaatc
     5221 cgttcttcca cagcgccggg tacaaggccg ggtggctggc cgcgctgatc cgcggcgcga
     5281 cgatcctgcc gcacccggtc ttcgatgcgg agcagatcct gctgcgcatt gcccaggagc
     5341 gaatcagctt cctgctcggc ccgccgacac tgttcctgac cctgctcgcc catccacggg
     5401 tgaaagagtt cgacctgagc tgcctgcggg tggcggtgac gggtgccgcc agtgtgccgc
     5461 ccgtgctggt gcgacgcatg cgcgatgagc tgggctttcg catcgtcgtc acggcatacg
     5521 gcctgaccga gtgctgcggc ttcgcgacga tctgcaatcc gcaggacgac gccgagacca
     5581 tcgccaacac ctgcggtcag gcgatccccg atgtcgaggt gcgctgcgcg gatccgcagg
     5641 gcaacacggt cccggcgggc gagccgggcg aggtgctgat ccgcggctac aacgtgatgc
     5701 agggctacct caacaacgag gccgccacgc gcgaagccat cgacgccgag ggctggctgc
     5761 acaccggcga cgtgggcgtg atggacgaca agggttatct gcgcatcacc gaccgcatga
     5821 aagacatgtt catcgtcggt ggtttcaact gctacccggc cgagatcgaa cggctcgcca
     5881 atgcccaccc cgcgatcgcc aacatcgccg tcatcggcgc ggcggacgaa cgccagggcg
     5941 aggtcgggcg cgcgttcatc gtgctgcgcc ccggcaccac cttgagcgcc gaagaattcg
     6001 ttgcctggtg ccgcgcccac atgacgaact acaaggttcc gcgctacgtc gacatcttgc
     6061 ccgcgctgcc gcttaatggc gcaggcaagg tcgacaagct cgcgctcagg gccatgccac
     6121 aggtcagccc catcgggcgc tgagcctcac cccaagagac cgcattcatg tcgcacttca
     6181 atcccgtttc agattcgccg atcaccgagg acgatgcctt cattgccagg gcactcgaga
     6241 aggccagcgt gcccgcgctg atgatgacgc tgacccacat cacgggccag agccgttggc
     6301 tcgacggtgc gatccgcccg aagaacccgt tgatcggcga gctgcaaggc tatctcagcg
     6361 atgccgagca ggcccaggtg cgcgccgaag cgcttcaggt gctcaaggcc taccgcgacg
     6421 gcggctcgca gatgccggca ccgcccgacg acgcgcggct gcttcaaatg ctgaacttca
     6481 tcgtcggcac cgaggtgccc gacgactatg tgccgatgat ccgggacgaa ctctcgctcg
     6541 gcggcgacga tgccaacagc gtcagactcg aacgtccgct ggcggaggac gagaaggcga
     6601 actaccacac gctggtgatc ggcgcgggct tgtccggcct gctgatgggc ctgcggctga
     6661 aggaactggg cgtgcccttc acgatcgtcg agaagaacga ctcggtgggc ggcacctg
//
LOCUS       DATMJB010000331         3378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_837692, whole genome shotgun sequence.
ACCESSION   DATMJB010000331 DATMJB010000000
VERSION     DATMJB010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837692"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VNU48_07210"
     CDS             complement(<1..754)
                     /locus_tag="VNU48_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010097661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWI11102.1"
                     /translation="MKAAMRRRPGEVRDAIVRVLEGRPQAASVQLIMQEVVSLIGEVP
                     ASSIRSYLRLNTPELFARMDRAQYTLQGFEGAGAAVAERPRTAERKFEFGGASIVLAD
                     CNAWLAAQPDDSIHAVVTDPPYGLVEYSDSEQDKLRSGRGGVWRIPPSFDGTARAPLP
                     RFTVLKPADLEALDRFFLAWGRQLVRILVPGANVLVASNPLLSYIVSGALARAGLERR
                     GELVRLTMTMRGGDRPKAAHVEFNDVSVMPRSM"
     gene            complement(844..>3378)
                     /locus_tag="VNU48_07215"
     CDS             complement(844..>3378)
                     /locus_tag="VNU48_07215"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11103.1"
                     /translation="FEPAVIERLLADVQQLHDDPDHLPLLQHVLARIWEAACARIAGD
                     MPAQIVWGDLERAVDPRATRAAGWLAAQEAVNTLRSSLENWAEAIYRERTPPNRALID
                     QVLRQLAFKDPNNGQYSQQRLDVDDPLLFAGVTQPRQLLRELLGRGFLDSVNYLFWDK
                     ENPERVTLKVSHESFIRGWAHFRALVDAEADRFDEFVAVLRKCALWKLDENPELLLES
                     SELMRFEGSGLAPVFSSRPEREGWFRVLRQYRDGERLAKVEPFVDAFIASSRARHEAE
                     EQARRDAAERERLSIEREREAERQRELQAREFAAESRRILAEKERAQAEARQAEAETD
                     RAEAVSKRNFWLVVTTMIVAFVLLPYAAFAILIQTPVMRSVEKFSTARTLLERRDRSD
                     TNPVAGAAGRELGVLLRAAALVEQAKQEVAFFDRRGMRDALDVLPPVGDAKRLFAMSS
                     SEPVVNGSLRALLTSGLWRSTTEAAAVDPALVSRPVRKELDCNVLGPAGTAQVLHGSV
                     FVANESGRGLFVPVPAAEDPDIAFYAASFVDERCSAGRIAWSVPRYFGPLVLVDARAR
                     YMAVALERPLSGPASVSLYTINWELGGDGQLPLAQMRFRSVVTDAGAFELVRREIRPD
                     PAVSVPEVAGIASWREPGGVGVTVAGRSWRVFNDGAQRIDRPGPPEAWAKLAAPSAGS
                     GCELLGKALGKKLQTGFRAEMFEDGNRCFEIQRGNPPAGPMMAPAAPDGAPAQARREQ
                     VLLAVYPQPRSEDLAAADATLPAAIASIAAFARVPVGDGEWLVGKSGEHEGWIALRRR
                     ARDGSEVYTGAPWSTRALERLGSQVLALANDPALALPPAAGASAPR"
BASE COUNT          504 a         1234 c         1171 g          469 t
ORIGIN      
        1 acatcgagcg cggcatcacg ctcacgtcgt tgaattcgac gtgtgctgcc ttggggcggt
       61 ctccgccgcg catcgtcatc gtcagacgca ccagctctcc gcgacgctcc aggcctgcgc
      121 gtgcgagcgc gcccgaaacg atgtacgaca gcagcgggtt gctggcaacc agcacgttgg
      181 cgcccggcac caggatccgc accagttgcc ggccccaggc cagaaagaat cggtcgagcg
      241 cctcgaggtc tgccggcttg agcaccgtga aacgcggcaa tggcgctctg gccgtgccgt
      301 cgaagctggg ggggattcgc cacacgccgc cgcggccgga gcgcagcttg tcctgctccg
      361 aatccgagta ttcgaccagg ccgtagggcg gatcggtcac gaccgcatgg atggaatcgt
      421 ccggctgggc ggcgagccag gcgttgcaat cggcaagcac gatcgatgcg ccgccgaact
      481 cgaacttgcg ttccgcggtc cgcggccgtt cggccaccgc ggccccggct ccttcgaacc
      541 cctgcagcgt gtactgcgcg cggtccatgc gggcgaacag ttccggcgtg ttcagccgca
      601 agtaggaccg gatcgacgaa gccggcacct cgccaatcag cgaaacgacc tcctgcatga
      661 tcagctgaac gctcgcggcc tgcgggcgtc cttcgaggac gcggacgatg gcatcgcgga
      721 cttctccggg gcggcggcgc atggcggctt tcaacaagta cacgtggagc gaagtataga
      781 cgtctggacg tctccagacg tttgaccggt cccgaaagac gatgaggtcc agcgcaaacg
      841 ccctcagcga ggcgcggacg cgcccgccgc tggcggcagc gccaacgccg gatcgttagc
      901 cagcgccagc acctgcgacc ccaggcgctc gagtgcgcgc gtgctccacg gtgcaccggt
      961 gtagacctcg ctgccgtcgc gcgcgcggcg ccgcagcgcg atccagccct cgtgctcgcc
     1021 gctcttaccg accagccact cgccgtcgcc caccggaacg cgcgcgaagg ccgcgatgct
     1081 cgcgatcgcg gccggcagcg tcgcgtcggc cgcggccagg tcttcggaac gcggctgcgg
     1141 gtagaccgcc agcagcacct gctcgcggcg cgcctgcgcg ggtgcgccgt ctggcgcggc
     1201 cggcgccatc atcgggccgg cgggcgggtt gccgcgctgg atctcgaagc agcggttgcc
     1261 gtcctcgaac atctcggcgc gaaaacccgt ctgcagcttc ttgcccagtg ccttgccgag
     1321 cagttcgcag ccgctgccgg cgctcggtgc ggcgagcttg gcccaggctt ccggcgggcc
     1381 gggccgatcg atgcgctgcg cgccgtcgtt gaagacgcgc cacgagcgcc cggcgacggt
     1441 cacgccgacc ccgccaggct cgcgccacga cgcgatgccc gcgacctcgg gcacgctgac
     1501 ggccgggtca gggcggatct cgcggcgcac cagctcgaac gcgccggcgt cggtgacgac
     1561 cgagcggaac cgcatctgcg ccagcggcaa ctggccgtcg ccgccgagct cccagttgat
     1621 cgtgtacagc gacaccgacg ccggcccgct gagcgggcgc tcgagcgcca ccgccatgta
     1681 gcgcgcacgc gcgtcgacca gcaccagcgg gccgaagtag cgcggcaccg accaggcgat
     1741 gcgcccggcc gagcagcgct cgtcgacgaa cgaggcggcg tagaacgcga tgtccgggtc
     1801 ctcggccgcg ggcaccggca cgaacagccc gcggcccgat tcgttggcga cgaacacgct
     1861 gccgtgcagc acctgggccg tgccggccgg acccagcacg ttgcagtcga gctccttgcg
     1921 caccggccgc gacaccagcg ccgggtcgac cgcggccgct tcggtggtgc tgcgccacag
     1981 gccgctggtc agcagcgcgc gcaggctgcc gttgacgacc ggctcggacg acgacatcgc
     2041 gaacaggcgc ttcgcgtcgc ccaccggcgg caggacatcc agtgcatcgc gcatgccgcg
     2101 ccgatcgaag aacgccacct cctgcttggc ctgctcgacc agcgccgcgg cgcgcagcag
     2161 cacgccgagc tcgcgaccgg cggcaccggc caccgggttg gtgtcggagc ggtcgcggcg
     2221 ttcgagcaag gtgcgcgcgg tcgagaactt ctcgacgctg cgcatcaccg gcgtctggat
     2281 caggattgcg aaggccgcat acggcagcag cacgaacgcg acgatcatcg tggtcacgac
     2341 gagccagaag ttgcgcttgc tgaccgcctc ggcgcggtcg gtctcggcct cggcctggcg
     2401 cgcctcggcc tgcgcgcgct ccttctcggc caggatgcgc cggctctcgg ccgcgaactc
     2461 gcgcgcctgc agctcgcgct gacgctcggc ctcgcgctcg cgctcgatcg acaatcgctc
     2521 gcgctcggcg gcgtcgcgcc gcgcctgctc ttcggcctcg tggcgcgcgc gcgagctggc
     2581 gatgaacgca tcgacgaacg gttcgacctt cgccaggcgc tcgccgtcgc ggtactgccg
     2641 cagcacacgg aaccagcctt cgcgctcggg gcggctcgag aacaccggcg ccaggccgga
     2701 gccctcgaag cgcatcagct ccgagctctc gagcagcagt tcggggttct cgtcgagctt
     2761 ccacagcgcg cacttgcgca gcaccgcgac gaactcgtcg aagcggtctg cctcggcgtc
     2821 gacgagcgcg cgaaagtgcg cccagccgcg gatgaacgac tcgtgcgaga ccttcagcgt
     2881 cacgcgttcg gggttctcct tgtcccagaa caggtagttg accgaatcga gaaagccgcg
     2941 gccgagcagc tcgcgcagca gctggcgcgg ctgcgtgacg ccggcgaaca gcagcgggtc
     3001 gtcgacatca agtcgctgct gcgaatactg gccgttgttc gggtccttga aggcgagctg
     3061 gcgcagcacc tggtcgatca gcgcccggtt cggcggcgtg cgctcgcggt agatcgcctc
     3121 ggcccagttc tcgaggctgc tgcgcagcgt gttgaccgcc tcctgcgccg cgagccagcc
     3181 ggccgcgcgc gtcgcacgcg ggtcgacggc acgctccaga tcgccccaga cgatctgcgc
     3241 cggcatgtca ccggcgatgc gcgcgcacgc cgcctcccag atgcgcgcga gcacgtgctg
     3301 cagcagcggc aggtggtcgg ggtcgtcgtg cagctgctgc acgtcggcca gcaagcgctc
     3361 gatcaccgcg ggttcgaa
//
LOCUS       DATMJB010000332         3381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_574425, whole genome shotgun sequence.
ACCESSION   DATMJB010000332 DATMJB010000000
VERSION     DATMJB010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574425"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VNU48_07220"
     CDS             <1..1164
                     /locus_tag="VNU48_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWI11104.1"
                     /translation="EAEGMLDASRAVVVTQDAQVTALIAGTSRTASVFMSDIATLATG
                     LNLTLSGRYNDTRVRTTDLGRSTLGLDTALDGTGRFSKFNPAAGLTWQVTPALTAYGG
                     FSQGNRAPSPIELGCSDPDHACVLPNALQGDPPLKQVVSRTLEAGVRGGLDADLRWNA
                     SVFRTDNRDDLLFVGNGRSAGYFANFGRTRRQGVELGVSQRGALVDWSVSYSYLRASF
                     ESSACLVSASNSSAGSSAACAADEIEVRPGNRLPGLPAHSAKFTVDARALPNWSVGAQ
                     LAAYSSLFVRGNENNAQQADGIDFFGSGKIGGFALVNLTTNWKLGSGWELFAKVSNLF
                     DRRYASGGLLAENAFDAHGSLLAPADWRNEQFVAPGAPRALWLGVRWSFGTPG"
     gene            1171..1323
                     /locus_tag="VNU48_07225"
                     /pseudo
     CDS             1171..1323
                     /locus_tag="VNU48_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1543..1812)
                     /locus_tag="VNU48_07230"
     CDS             complement(1543..1812)
                     /locus_tag="VNU48_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HWI11105.1"
                     /translation="MPSTTTTKPSAFMKALKPSTALAAVVGSEPLPRTEVTKRLWDYI
                     KANNLQNPANKRNILCDGQLRAVMGKDEVTMFEMTGLVGKHLGPV"
     gene            2055..3098
                     /locus_tag="VNU48_07235"
     CDS             2055..3098
                     /locus_tag="VNU48_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HWI11106.1"
                     /translation="MIPDNAYAELGLAPGASDDEVKTAWRRLASQWHPDRNRSAAAIG
                     RMQRINQAIEQIRQFDGGQAARDESNETPAGDADAAGSQAQVRTISRKVKLTLEEAAA
                     GCVKALRGKLIDRCAPCAGTGFCVIGGACTECKGRGSVRQPAWFGWSGASTRCTACGG
                     DGVARQGCDACSASGKLPPREYRINVRIPHGVRDGDLLHVGQRRQSSGHPAVQLNLRV
                     EVLPHALFELDADGTIRCEVPVDGFAWIANRSIDVPTLTGMQGLQLSRDCLSYRLGGQ
                     GFPKERRGPCGDHLVELAPIFPERLGTDQEILLDQLIAASSGPGGSAAATRLGDWRRA
                     LQSWELGRTPRGR"
     gene            complement(3119..>3381)
                     /locus_tag="VNU48_07240"
     CDS             complement(3119..>3381)
                     /locus_tag="VNU48_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11107.1"
                     /translation="ASIDLPGRGYGYQWWTNNDGSFAAQGIFGQGIFIDPKRKLVIAS
                     NADWAAGATDRVASAAREAFYRAVQKSIDDEAAGLPNAESAR"
BASE COUNT          504 a         1151 c         1178 g          548 t
ORIGIN      
        1 gaggccgagg gcatgctcga cgcgtcgcgc gccgtcgtcg tgacgcagga cgcccaagtc
       61 acggccctga tcgccggcac cagccgcacc gcgagcgttt tcatgtccga catcgcgacg
      121 ctcgcgacgg gcctgaacct cacgctgtcc ggccgctaca acgacacgcg cgtgcgcacc
      181 accgatctcg gccgcagcac gctgggcctg gacaccgcgc tcgacggcac gggccggttc
      241 agcaagttca acccggccgc ggggctgacc tggcaggtca cgccggcgct gacggcgtac
      301 ggcggcttca gccagggcaa ccgggcgccc agcccgatcg agctcggctg ctccgacccc
      361 gaccacgcct gcgtgctgcc gaatgcgctg cagggcgatc cgccgctgaa gcaggtcgtc
      421 tcgcgcacgc tcgaggccgg cgtgcgcggc ggcctcgatg ccgacctgcg ctggaacgcc
      481 tcggtgtttc gcaccgacaa ccgcgacgac ctgctgttcg tcggcaacgg ccgctcggcc
      541 ggctacttcg cgaacttcgg ccgcacgcgg cgccagggcg tggagctcgg ggtctcgcag
      601 cgcggcgccc tggtcgactg gagcgtctcg tacagctacc tgcgtgcgag cttcgaatcg
      661 tcggcctgcc tggtgtcggc gtccaacagc agcgccggca gcagcgcggc ctgcgcggcc
      721 gacgagatcg aggtgcggcc cggcaaccgc ctgccgggcc tgccggcgca cagcgccaag
      781 ttcacggtcg acgcgcgcgc gctgcccaac tggagcgtgg gcgcgcagct cgccgcgtat
      841 tccagcctgt tcgtgcgcgg caacgagaac aacgcccagc aggccgacgg gatcgatttc
      901 ttcggcagcg gcaagatcgg cggcttcgcg ctcgtgaacc tgacgacgaa ctggaaactc
      961 ggcagtggct gggagctgtt cgcgaaagtc tcgaacctgt tcgaccgacg ctatgccagc
     1021 ggcggcctgc tggccgagaa tgccttcgat gcgcacggca gcctgctggc gccggccgac
     1081 tggcgcaacg agcagttcgt cgccccgggc gcgccgcgcg cgctgtggct tggggtacgc
     1141 tggagcttcg gcacgcccgg gtgagccgcg atggcggcgg tgatgaacgg cgtgatgctg
     1201 cacggcggct tccggccgtt cggcggcacc ttcctgacct tctcggacta cgcgcgcaac
     1261 ggtgtgcgca tgtcggcgtt gatgaagctg ccggtcgtct acgtgttcac gcacgactcg
     1321 atgagcgcga cggattgcga ggcctgttct tgcgtacagt actgtttgaa cgtacagttc
     1381 actcaacagg agccgatcat gcacacatcc ctgcgtcaga actatgtacc gaagccggtc
     1441 cgcgcgcccg tctggctgcg ccgcatctgg gcttggctct gaccactgcc tccggtcttt
     1501 cccgtgggcc ctggtcgagt cgggctccca aaggggctcg ggtcagaccg gtcccagatg
     1561 cttgcctacc aagccggtca tctcgaacat ggtgacctcg tccttgccca tcacggctct
     1621 cagctggcca tcgcaaagga tgttgcgctt gttcgctggg ttttgcagat tgttggcctt
     1681 gatgtagtcc cacaggcgct ttgtcacttc ggtgcggggc agcggctcgg aaccgacgac
     1741 cgcagccagt gcggtgctgg gtttgagggc cttcatgaaa gcgctgggct ttgtcgtggt
     1801 cgtgctgggc atgggcgctt gtgatggtgg gtcaggggag gcggagcata cgccctggat
     1861 cgataacccg ctttggcgtt gtccgatatc actgccgacg cgaccggcgg gccactcggg
     1921 gatttcaccc ggcccaaggc ataatttggt gcgccgcagc aatgcgagcc tgcgccacga
     1981 aggcctcgat ggcggcgccg tcccggtccg tccgcgcgca gcggctgacc gccaagagac
     2041 caaggagcgc gggattgatt ccggacaacg catacgccga actgggcctg gcccccggcg
     2101 cttcggacga cgaggtgaag acggcgtggc gtcggctcgc ctcgcaatgg catcccgatc
     2161 gcaatcgaag cgccgcggcg atcggcagga tgcagcgcat caatcaggcg atcgagcaga
     2221 tccgacagtt cgacggcggc caggctgccc gcgacgagag caatgagaca cctgccggcg
     2281 acgccgacgc cgcgggatcg caggcgcagg tgcgaaccat cagccgcaag gtcaagctga
     2341 cgctggaaga ggccgccgcg gggtgcgtca aggcgctgcg tggcaagttg atcgaccgct
     2401 gcgcaccttg cgcaggcacc ggattttgcg tgatcggggg cgcctgcacc gagtgcaagg
     2461 gcaggggctc cgtccgacag cccgcctggt tcggctggtc gggcgcgtcg acccgctgca
     2521 ccgcctgcgg cggcgacggc gtcgccagac agggttgcga tgcctgcagc gccagcggga
     2581 agctgccgcc gcgcgagtac cggatcaacg tgcgcattcc gcatggcgtg cgtgacggtg
     2641 acctgctgca cgtcggccag cgccgccaga gctcgggcca ccccgccgtg caactgaacc
     2701 tgcgcgtcga ggtgctgccc catgcgctgt tcgaactcga cgccgacggg acgattcgtt
     2761 gcgaggtgcc tgtcgatggc ttcgcgtgga tcgcgaaccg gtcgatcgat gtgcccacgc
     2821 tgaccggcat gcaaggtctg cagctgagcc gcgactgcct gtcgtatcgg ctcggcggtc
     2881 agggctttcc gaaggagcgg cgcggtccgt gtggcgatca cctcgtggag cttgcgccga
     2941 tctttccgga acgactcggc accgaccagg agatcctgct ggaccagctc atcgcggcct
     3001 cgtcgggacc gggcggctcg gccgcggcca cgcgcctcgg cgactggcgg cgcgcgctgc
     3061 agagctggga actggggcgc acgccgcgcg gccgctgact accgccgcgt cggcgtcgtc
     3121 atcgcgccga ctcggcgttc ggcaggcccg ccgcctcgtc gtcgatggac ttctgcaccg
     3181 cgcggtagaa cgcctcgcgt gccgcgctgg cgacgcggtc ggtcgcccct gcggcccagt
     3241 cggcgttcga ggcgatcacg agcttgcgct tcgggtcgat gaagatgccc tgaccgaaga
     3301 tgccctgagc ggcaaagctg ccgtcgttgt tcgtccacca ttgatagcca tagccgcgcc
     3361 ccggcaggtc gatcgacgcc t
//
LOCUS       DATMJB010000333         2415 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_845621, whole genome shotgun sequence.
ACCESSION   DATMJB010000333 DATMJB010000000
VERSION     DATMJB010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_845621"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VNU48_07245"
     CDS             <1..552
                     /locus_tag="VNU48_07245"
                     /inference="COORDINATES: protein motif:HMM:NF025179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNVR domain-containing protein"
                     /protein_id="HWI11108.1"
                     /translation="VLDKQTEALIAGAAQVRAQIAEREVQLKVLRTGSTDQNADVIRL
                     RAEIAALRTELARMEGGGTGSMIDIPMGKLPAAGVEYVRARRELKLQETLLEAMVRQY
                     ELAKVDEAREGPLLQQIDRAVPPDYKSKPSRALLVIIVTLLGLVGSIAWVVIRRFAAL
                     ARERDAEGAAAWRAMKHAWRLRG"
     gene            636..971
                     /locus_tag="VNU48_07250"
     CDS             636..971
                     /locus_tag="VNU48_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family EPS-associated transcriptional
                     regulator"
                     /protein_id="HWI11109.1"
                     /translation="MLPDDFQYELMRQITDQPDASQRSLATRLGVSVGKVNYCLRALV
                     DKGWVKANNFRRSDRKWAYAYLLTPRGASAKVRLARDFLANKEREYERLHHEIALLRN
                     ELLQQRPDA"
     gene            968..1942
                     /locus_tag="VNU48_07255"
     CDS             968..1942
                     /locus_tag="VNU48_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HWI11110.1"
                     /translation="MKRDAKIFVTGHRGMVGSAIVRRLQAGGYTNLVTRTRAELDLLD
                     QRAVHAFLGADQPDYLFIAAATVGGIQANNLRRADFLYENLLIEANLMHGAHRAGVQR
                     LMFLGSSCIYPRDCPQPIKEDYLLTGPLEPTNEPYALAKIAGIKLAESYNRQHGRQYV
                     SVMPTNLYGPNDNYDLATSHVLPALLRKAHEAHARGDAEFVVWGSGTPRREFLYVDDL
                     ADACVHLMETGYDGPLVNVGTGEDVTIRELARAVMRVVGFEGRIVFDASKPDGTPRKL
                     LDVSRLAALGWRARTGLPDGIRAAYESAPFHARRGAAAAGSQSTRTTP"
     gene            1939..>2415
                     /locus_tag="VNU48_07260"
     CDS             1939..>2415
                     /locus_tag="VNU48_07260"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWI11111.1"
                     /translation="MTQPKVALITGVTGQDGAYLSELLLYKGYEVHGIKRRASLFNTD
                     RIDHLYQDPHVDHQRFKLHYGDLTDSTNLIRIIQQVQPDEIYNLAGMSLVSASWRQPV
                     LTAECTGLGATRLLEAQREAVPDARFFQASSAEMFGLAVEVPQRETTPLCPRSPYAA"
BASE COUNT          430 a          850 c          787 g          348 t
ORIGIN      
        1 gtgctcgaca agcagaccga agccctgatc gccggcgccg cacaggtccg ggcccagatc
       61 gccgaacgcg aagtccagtt gaaagtgctg cgcactggca gcaccgatca gaacgccgac
      121 gtgatccgcc tgcgcgccga gatcgccgcg ctgcgcaccg agctcgcacg gatggagggc
      181 ggcgggacgg gcagcatgat cgacatcccg atgggcaaac tgcccgcggc cggcgtcgag
      241 tacgtgcgcg cccgccgcga actgaagctg caagaaaccc tgctggaggc gatggtgcgc
      301 cagtacgaac tcgccaaggt cgacgaagct cgcgaggggc cgttgttgca gcagatcgat
      361 cgggcggtgc caccggacta caagtccaag ccgtcgcgag cgctgctggt gatcatcgtg
      421 acgctgctcg ggctcgtcgg ctcgatcgcc tgggtcgtga tccgccgctt cgcagccctt
      481 gcccgtgagc gcgacgccga aggcgcggcc gcgtggcgtg cgatgaagca cgcctggcgc
      541 ctgcggggct gatcgcgcgt tgttgcgggc ggtcggccca gcccgcaaac gcgggtatca
      601 tccgcaccgt tcaaacgttg aacacatccg atttcatgtt gccggacgac ttccaatacg
      661 agttgatgcg ccagatcacg gaccagcccg atgcgagcca gcgcagcctg gccacgcggc
      721 tgggggtcag cgtcggcaag gtcaactact gcctgcgcgc actcgtcgac aagggctggg
      781 tcaaggcgaa caacttccgc cgcagcgacc ggaaatgggc ctatgcctac ctgctgacgc
      841 cgcgcggcgc cagcgccaag gtgcggctgg cgcgcgactt cctggccaac aaggaacgcg
      901 agtacgagcg cctgcaccac gagatcgcgc tgcttcgcaa cgagctgctg cagcagaggc
      961 ccgacgcatg aagcgcgacg ccaagatttt cgtcaccggg caccgcggca tggttggcag
     1021 tgcgatcgtg cgacgcctgc aagcaggcgg ctacaccaat ctggtcacgc gcactcgggc
     1081 cgaactcgac ttgctcgatc agcgcgccgt gcacgccttc ctgggcgcgg accagcccga
     1141 ctacttgttc atcgctgccg ccacggtcgg cgggattcag gccaacaacc tccggcgcgc
     1201 cgacttcctg tacgagaacc tgctgatcga ggccaatctg atgcacggcg cgcaccgggc
     1261 cggcgtgcag cgattgatgt tcctgggctc gagctgcatc tacccgcgcg attgcccgca
     1321 accgatcaag gaagactacc tgctgaccgg gccgctcgag cccaccaacg agccctatgc
     1381 gctcgccaag atcgccggca tcaagctggc cgagagctac aaccgccagc acggccggca
     1441 gtacgtcagc gtgatgccga ccaatctgta cggcccgaac gacaactacg atctggccac
     1501 cagccacgtg ctgccggcgc tgctgcgcaa ggcgcacgag gcgcacgcgc gcggcgatgc
     1561 cgaattcgtc gtctggggca gcggcacacc gcgccgcgag ttcctctacg tcgacgacct
     1621 cgccgacgcc tgtgtgcatt tgatggaaac cggctacgac gggccgctcg tgaacgtcgg
     1681 caccggcgag gatgtgacga tccgcgaact cgcccgggcc gtgatgcgcg tggtcggctt
     1741 cgagggccgc atcgtgttcg atgccagcaa gccggacggc acgccacgca agctgctcga
     1801 cgtgagccgt ctcgcggcgc tcggctggcg cgcgcgtacc gggttgccgg acggcatccg
     1861 cgccgcctac gagagcgcgc cgtttcacgc caggcgcggc gcggccgcgg ccggcagcca
     1921 gtcaacgcgg accacaccat gacgcaaccc aaggtcgccc tcatcaccgg cgtcaccggc
     1981 caggacggcg cgtacctgtc cgagctgctg ctctacaagg gctacgaggt gcacggcatc
     2041 aagcgccgcg ccagcctgtt caacaccgac cgcatcgacc acctgtacca ggacccgcat
     2101 gtcgatcacc agcgcttcaa gctgcactac ggcgacctga ccgactcgac caacctgata
     2161 cgcatcatcc agcaggtgca gcccgacgag atctacaacc tggcggggat gtcgctggtg
     2221 tcggcgtcct ggcgacagcc ggtgctgacc gccgagtgca ccggcctcgg cgcgacccga
     2281 ctcctggagg cacagcgcga agcggtaccc gatgcccgtt tcttccaggc gtcctccgcc
     2341 gagatgttcg ggcttgccgt ggaggtcccg cagcgggaga ccacaccctt gtgcccccgg
     2401 agcccgtacg cggcg
//
LOCUS       DATMJB010000334         2762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_179064, whole genome shotgun sequence.
ACCESSION   DATMJB010000334 DATMJB010000000
VERSION     DATMJB010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179064"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VNU48_07265"
     CDS             complement(<1..355)
                     /locus_tag="VNU48_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWI11112.1"
                     /translation="MKAKRMALGLLGALVAAAFAGWFSLDKETRALLATLPTNRDVLF
                     WSQPQRDAAFRALDRLPVLAKSRVVPAGGAPLPLPTGAPLKLALDIDAYMAGQRSAAL
                     LVVHDGRLRLERYGLG"
     gene            complement(419..1147)
                     /locus_tag="VNU48_07270"
     CDS             complement(419..1147)
                     /locus_tag="VNU48_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11113.1"
                     /translation="MCADSRLSRVAVLAFAGLLSALASSGARCAELSATEQRWLQGAW
                     PVVAYAKQARLPLDVVVQPQATPDQPPLSTAFVDGRCKLVLSMRGNPQAQATLDAIEP
                     ALLNATLELMAAHELGHCRRYLDGAWFQLPVGFVGGVETDTSRDAAPRREEGYGDLVG
                     LAWTRSRHPELYPRLHAWLLNERSTERVPGSPHDTVAWVQLAQDPATLGRGELFDAPK
                     SLWIQGAVVPVAPRDEECRAPCPD"
     gene            complement(1372..1587)
                     /locus_tag="VNU48_07275"
     CDS             complement(1372..1587)
                     /locus_tag="VNU48_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11114.1"
                     /translation="MTYYARVVYDSNASVAERHDDVGAARCWIEAEKNASPTSFRLGQ
                     ILQGSPVPEIVATCDTKGWRTSQEDGA"
     gene            1994..>2762
                     /gene="oxlT"
                     /locus_tag="VNU48_07280"
     CDS             1994..>2762
                     /gene="oxlT"
                     /locus_tag="VNU48_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HWI11115.1"
                     /translation="MNRPLAATAHTFGSVPAVATRSPYYRWMQLLMGIVCMAMIANLQ
                     YGWTLFVNPIADKHGWSRAAIQVAFTIFVLTETWLVPIEGYLVDRFGPRPVVLVGGLL
                     CGLGWVLNSYADTLTLLYIAAAVGGVGAGAVYGTCVGNALKWFPDRRGLAAGLTAAGF
                     GAGSALTIVPISMFIKSNGYEAAFLYFGIAQGLIVFLLAWFLTPAPEHKKAAGEVRIN
                     VQQSKREYSPAEVVRKPVFWVMYLMFVMVAAGGLMATA"
BASE COUNT          447 a          938 c          930 g          447 t
ORIGIN      
        1 agcccaggcc gtagcgctcc aggcgcagcc tgccgtcgtg cacgaccagc agggccgcgc
       61 tgcgctggcc cgccatgtag gcatcgatgt ccagcgccag cttcagcggc gcgcccgttg
      121 gaagcggcag cggcgcgccg cccgccggca cgacccgcga cttggccagc acgggcagcc
      181 gatcgagcgc gcgaaacgcc gcatcgcgct gtggctggct ccagaacaga acatcgcgat
      241 tcgtcggcag cgtcgccagc agggcgcgcg tctccttgtc gagactgaac cagccggcga
      301 aagctgccgc gaccaacgcg cccagcagcc cgagcgccat cctctttgct ttcatcgcag
      361 ggttccggat ccatcgtgaa gatggaccgg attgaacacg atttgcgaac tcgcgccgtc
      421 aatcggggca gggcgcacga cattcttcgt cgcgcggcgc caccgggacg acggcgccct
      481 ggatccaaag cgacttcggc gcgtcgaaca gctcaccccg gcccagcgtt gcagggtcct
      541 gcgcgagttg cacccacgcg accgtgtcgt gcggactgcc gggcactcgc tcggtcgagc
      601 gttcattcag cagccaggcg tgcaggcgcg ggtagagctc gggatgccgg cttcgggtcc
      661 aggccaggcc gacgaggtcg ccgtaacctt cttcgcggcg tggggccgca tcccgcgagg
      721 tgtcggtctc cacaccaccg acgaatccca ccggcagctg gaaccaggcg ccgtccaggt
      781 agcggcggca gtggcccagc tcatgcgccg ccatgagctc gagcgtggcg ttcagcagcg
      841 ccggctcgat ggcgtcgagc gtggcctgcg cctgcgggtt gccgcgcatc gacagcacga
      901 gcttgcagcg gccgtcgacg aaggccgtgg acagcggcgg ctggtcgggt gtggcctgag
      961 gctgcaccac gacgtccagt ggcagccgcg cctgcttcgc gtacgcgaca accggccagg
     1021 cgccctgcag ccagcgttgc tcggtggcgc tcagttcggc acagcgtgcc ccgctcgagg
     1081 ccagagccga caacaggccg gcgaacgcca gcacggcaac gcgagacagt cgggaatcgg
     1141 cgcacatcgg gcagctcctt accgaggact tcggtacatc cgcaggaggg ttgagctcat
     1201 cccggttccc caccgacacc gctgcgcacg ggcgtcgaca agtgcacggg aatcgagatc
     1261 gaggtcgcga tcgtggcgcg tggcgggcga cccgtgggca cgcgtgtcgg cgcgggtcta
     1321 caacgacatg agcgacatcg aacgactcgg cgaatccgtc gggccctccg actacgcacc
     1381 gtcttcctgc gacgtacgcc agcccttcgt gtcgcaggtc gcgacgattt cggggaccgg
     1441 actgccctgg aggatctgac ccagacggaa cgacgtcggg ctggcgttct tctccgcctc
     1501 gatccagcaa cgcgccgcgc caacgtcgtc atgcctttca gcgaccgacg cattgctgtc
     1561 gtagacaacc ctggcgtagt aggtcatgtg agccgtcgct aggtgtggcc gcccgttgaa
     1621 tgtcgatgct gcgctgcagc aatgtcgcac aaacagaatc gttcgacaag ggccgcgcgc
     1681 cgatgcgttg tggcgccgga cgacgatcga cgcgccgcgc aaaagtcacc tcatgggtgg
     1741 ttacccgagt cgttgaccaa ggtcaagaga attcgcagcg gaaagtctga cagtgaaccc
     1801 actccggtag cgtctgcgca gccgggatgc ctgccaagcc gctgctgcgc ggcgcaggcg
     1861 ccgagttcgg aatccgtcga agactggccg cgcgactcat ctgctcaagc gcctcttttc
     1921 gattcaagcc cggtgtgcgc ggtcgcccga cacgccctgc gacaggcctt cagaaaattg
     1981 tcaggagacg tcaatgaatc gacccctcgc cgccacggcg cacaccttcg gaagcgttcc
     2041 tgcggtcgcc acccgcagcc cctattaccg ctggatgcaa ttgctgatgg gcatcgtctg
     2101 catggcgatg atcgccaacc tgcaatatgg ctggacgctg ttcgtcaacc cgattgccga
     2161 caagcacggc tggagccgtg cggccatcca ggtggccttc acgatcttcg tgctgaccga
     2221 gacctggctg gtgccgatcg aaggctacct ggtcgatcgc ttcgggcccc ggccggtggt
     2281 gctggtcggc ggcctgctgt gcggcctggg ctgggtgctg aactcctacg ccgatacgct
     2341 gacgctgctg tacatcgcgg ccgcggtcgg cggcgtcggt gcgggcgcgg tgtacggcac
     2401 ctgcgtcggc aatgcgctga agtggtttcc ggatcgccgc ggcctcgcgg ccggcctcac
     2461 ggccgccggc ttcggcgccg gctcggcgct gacgatcgtg ccgatctcga tgttcatcaa
     2521 gtccaacggc tacgaggccg cgttcctgta cttcggcatc gcccagggcc tgatcgtgtt
     2581 cctgctggcc tggttcctga cccccgcgcc cgagcacaag aaggcggccg gcgaggtgcg
     2641 gatcaacgtg cagcagagca agcgcgagta cagcccggcc gaggtcgtgc gcaagccggt
     2701 gttctgggtc atgtacctga tgttcgtgat ggtggcggcc ggcggcctga tggcgaccgc
     2761 gc
//
LOCUS       DATMJB010000335         2894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_598470, whole genome shotgun sequence.
ACCESSION   DATMJB010000335 DATMJB010000000
VERSION     DATMJB010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598470"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VNU48_07285"
     CDS             <1..281
                     /locus_tag="VNU48_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11116.1"
                     /translation="LPEAHRAAAGTGAVDAAADPLARLIAAGVRFEAGRADPALIASA
                     VDTASSQGWRRPLLAWLGVQLKLAEQAGASDAAQRLRRRVELVQGAPG"
     gene            complement(398..667)
                     /locus_tag="VNU48_07290"
                     /pseudo
     CDS             complement(398..667)
                     /locus_tag="VNU48_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(585..1313)
                     /locus_tag="VNU48_07295"
     CDS             complement(585..1313)
                     /locus_tag="VNU48_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11117.1"
                     /translation="MTWTDETLMAFADGELADPVRDDIERAIAADAALRGRVAALRRQ
                     RARLAAAFAPVLDEPMPPRLAELLRDAEPPKVVPLDARRAARERPRMSATSWARWGGM
                     AAALLLGVLLGTQLERGGPTIGPRDARLVAEGPIGDALSSQLASAPVAGAPVAVQLSF
                     VDKSGRYCRTFSTSALAGMACRQDGQWDLQTLAAADPASATGMRQAASALPRAVLDAV
                     DRSIAGAALDAAGERRARDAGWHR"
     gene            complement(1313..1819)
                     /locus_tag="VNU48_07300"
     CDS             complement(1313..1819)
                     /locus_tag="VNU48_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWI11118.1"
                     /translation="MTDSSSDTVRRDLAALLPRLRRFGRTLAARREDADDLVQIAVER
                     ALRRADQWQPGTRLDSWMFRIMQNAWIDETRARERRSQTFVAEEEGERVGVSTTDAQI
                     DAIAVRKAVAQLSEDQRAVVGLVLVDGLAYKEAAEVLGVPIGTLTSRLARAREALQAI
                     LSDPEGAR"
     gene            1891..>2894
                     /locus_tag="VNU48_07305"
     CDS             1891..>2894
                     /locus_tag="VNU48_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWI11119.1"
                     /translation="MICAAPLLRRFRRLLPAALAGLAFAAPAHAQLGLPRVTAPTLPR
                     ELLPRVGTTTAPLGETLQQGLVPLQWLRQSTVRALLRDHPDVLEADPSGEPMRRGELI
                     LASPAAATVAAALDQGFALLREQAWPELDLREVVLRAPPGVPTAEALRRLRAIDPQLE
                     ADFNHLYTRSGEVKPEATAAGAGAATGARRVGLIDSGVERGHAAFRGAELRSWGCDDV
                     AVPSPHGTAVASLLIGRNAAFAGVATGSMLYAADIYCDRPSGGAVETIVQALAWMDRE
                     QVGVVNISLVGPANRLLERAARAMNQKGHLLVAAVGNDGPAAPPLYPASYPGVVGVTA
                     V"
BASE COUNT          346 a         1095 c         1066 g          387 t
ORIGIN      
        1 tgttgcccga agcgcatcgc gcggcggccg gcaccggcgc ggtcgacgcc gccgccgacc
       61 cgctcgcgcg cctgatcgcg gccggcgtgc gcttcgaggc cgggcgcgcc gacccggcgc
      121 tgatcgccag cgccgtcgac accgcatcga gccagggctg gcgccgcccg ctgctggcct
      181 ggctcggggt gcagctcaag ctggccgagc aggccggcgc gagcgacgcg gcccagcgcc
      241 tgcgccgccg cgtcgaactc gtccagggcg caccgggttg atcggtcagc gcgccgcgcg
      301 ggccaccgcc ggcactgctg aacccgatcg aggtgacgcc gcggtcgctg cgcgcgctga
      361 ccatgacgcc gtgcatgagc gcgaccgcct tgacgatcgc cagcccgaga ccgtggccat
      421 ggtgctcgcc ggcgtcgacg cggtagaagc ggtcgaacag gcgcggcagg tgcgccaccg
      481 cgatcggcgc gcccgggttc gtgacgttga tccagaccgc gtcggcctgg cgcgcgatgc
      541 tcaccgcgag ccgcgcgcgg ggcattgtcc ggcgctgcca gcgctcagcg atgccagccc
      601 gcgtcgcgcg cccggcgctc gccggctgca tcgagcgcag cgccggcgat ggaccgatcc
      661 accgcatcga gcacggcgcg cggcagcgcg ctcgcggcct ggcgcatgcc ggtggccgat
      721 gccgggtcgg cggcggccag cgtctgcagg tcccactgcc cgtcttgccg acacgccata
      781 ccggccagag ccgaggtgct gaaggtgcgg cagtagcgcc cggacttgtc gacgaagctc
      841 agctgcaccg ccaccggcgc gcccgcgacc ggcgcactcg ccagctgcga cgacagcgcg
      901 tcgccgatcg gcccttcggc cacgagccgc gcgtcgcgcg ggccgatcgt cgggccgccg
      961 cgctcgagct gcgtgccgag caggacgccg agcagcaagg ccgccgccat gccgccccag
     1021 cgggcccatg acgtagcaga catgcgcggc cgctcgcgtg ccgcgcgccg cgcgtccagc
     1081 ggcaccacct tcggcggttc ggcgtcgcgc agcagctcgg ccagacgcgg cggcatgggt
     1141 tcgtccagca ccggcgcaaa ggccgcggcc agccgggcgc gctgccggcg cagcgccgcc
     1201 acgcggccgc gcagcgcggc atcggcggcg atcgcgcgct cgatgtcgtc gcgcaccggg
     1261 tcggcgagtt cgccgtcggc gaaggccatc agggtttcat cggtccaggt catcatcggg
     1321 ctccttcggg gtcggacagg atcgcctgca gcgcctcccg tgcgcgcgcc agccggctgg
     1381 tcagcgtgcc gatcggcacc cccagcacct cggctgcctc cttgtaggcc aggccgtcga
     1441 cgaggacgag gccgaccacc gcgcgctggt cctcgctcag ttgcgccacc gccttgcgca
     1501 ccgcgatcgc gtcgatctgc gcgtcggtgg tggacacgcc gacgcgctcg ccttcctctt
     1561 cggcgacgaa ggtctggctg cgtcgttcgc gcgctcgcgt ttcgtcgatc caggcgttct
     1621 gcatgatgcg aaacatccag ctgtccaggc gcgtgcccgg ctgccattgg tcggcgcggc
     1681 gcagcgcccg ctcgacggcg atctgcacca ggtcgtcggc gtcctcgcgg cgcgctgcga
     1741 gcgtgcgccc gaaacgccgc aggcgcggca gcagcgcggc gagatcgcgg cgaacggtgt
     1801 ctgaggaaga atcggtcatc acttgctggg tacaacgcct ggcggcggga ttttcttcca
     1861 tgcctcgttc gttatgctgc tagtcgaatg atgatttgcg ccgccccact cctgcgccgg
     1921 ttccggcgcc tgctgcctgc tgcgctggcg gggctcgcgt tcgccgcgcc ggctcacgcc
     1981 cagctcggcc tgccgcgggt gaccgcgccg accctgccgc gcgaactgtt gccgcgcgtg
     2041 ggcacgacga ccgcgccgct gggcgagacg ctccagcagg ggctcgtccc cttgcagtgg
     2101 ctgcgccaga gcacggtgcg cgcgctgctg cgcgatcacc ccgacgtgct ggaggccgat
     2161 ccgtccggcg aaccgatgcg ccgcggcgag ctgattctgg cgtcgcccgc ggcggccacc
     2221 gtggccgccg cgctggacca gggcttcgca ctgctgcgcg agcaggcgtg gccggaactc
     2281 gatctgcgcg aggtggtgct gcgcgcgccg cccggcgtgc ccaccgccga ggccctgcgt
     2341 cgcctgcggg cgatcgatcc gcagctggag gccgacttca accacctgta cacgcgcagc
     2401 ggtgaggtga agcctgaggc gacggccgcg ggcgccggcg ccgcaaccgg cgcgcgccgc
     2461 gtcggcctga tcgacagcgg cgtcgagcgc gggcacgcgg cgttccgcgg cgccgagttg
     2521 cgcagctggg gctgcgacga cgttgccgtg ccgagcccgc acggcaccgc ggtggcgtcg
     2581 ctgctgatcg gtcgcaatgc ggcgttcgcc ggcgtggcga cgggctcgat gctgtacgcg
     2641 gccgacatct actgcgaccg tcccagcggc ggcgcggtcg agacgatcgt gcaggcgctg
     2701 gcctggatgg accgcgaaca ggtcggcgtc gtcaacatca gcctggtcgg cccggcgaac
     2761 cgcctgctcg aacgcgcggc ccgcgcgatg aaccagaagg gccacctgct cgttgccgcg
     2821 gtcggcaacg atggtccggc ggcgccgccc ttgtacccgg cgtcctaccc cggcgtcgtc
     2881 ggcgtcaccg cggt
//
LOCUS       DATMJB010000336        11116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_719102, whole genome shotgun sequence.
ACCESSION   DATMJB010000336 DATMJB010000000
VERSION     DATMJB010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11116
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_719102"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VNU48_07310"
     CDS             <1..218
                     /locus_tag="VNU48_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11120.1"
                     /translation="FGFHGLYCPLPLVSRTSEQGQRERTTCDIPQASQARHDRAEIIN
                     IDENSGSAQVGPWRRTRRSRPSLTASS"
     gene            557..3574
                     /locus_tag="VNU48_07315"
     CDS             557..3574
                     /locus_tag="VNU48_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWI11121.1"
                     /translation="MSQRLNRRILLADDMPAIHADFRKVLAPTTPALNLDEVESALFG
                     TAAQSTAVAFELDIAHQGQEALAKVREALRVGRPYALAFIDMRMPPGWDGVETIEQLW
                     REDPRLQVVICTAYADQSWAEVVDRLDASDRLLVLKKPFDPIEVRQLAAALTAKWQMT
                     EDANAELQRRAELLVQAEEIALIGSMETNLVNGETTLSIGLCHLFGEPAITAPVAPTW
                     LCARVPDAERPLVQSIRDSVRTDEPFEFQHRIVRRDGVIRTVLHRCRVELGDDGTPLR
                     LHATLQDITSRREAEQALHDLAHADLATGLPNRHALLNRLSEATLAAGREDRGLSLLV
                     MEIDRLGLARDSLGHAGGEHLLKAVAQRLLKLASGAGMIAHLGGGEFALLVDASGEAA
                     AQSMARALVDGVARPFHIGSAEIYASVAVGVALWPTDADGPDELLDRACASAQRARES
                     GSNRICFYTPEANARTATRLANETGLRRALDRNELYLSYQPQVDLGSGRVIGLEALAR
                     WKDATRGDISPADFIPLAEETGLIVPIGEWVLRTACMDSMRWQAEGLAPVRVAVNLSM
                     LQLQQPDIVQRIQAILQETGLNPRHLGVEVTESMVMEQVEHVAQTLSQLRAIGVEIAL
                     DDFGTGYSNLSYLSKLPIDVLKIDRSFVHEVTAPTQDVSITRALINMAHGLNMKVLAE
                     GVENEGQLALLVASRCDQIQGYYFSRPARADAIAAMLREDKRLPEHLLVRRTSKRTLL
                     LVDDEDNIIASLRRLLRRDGYHIITANSGAQGLQRLAEHEVDVILSDQRMPGMTGVEF
                     LRRAKELYPQTVRMTLSGYTELQSIADAINEGAVYKFLTKPWDDERLRAHVNEAFRHK
                     EIADDNRELGARLQAAHDELATVNARLHRLTDMQLQQIRRDEVRLLSAHELLEGIPTP
                     VIGFDVEGVVAYLNADAHELFPPNDSPLGRAATEVLSPELTQVWHAGDGAQVCVRLGA
                     LHFQAVCRPIGDEPNQRGKLMVLSPHSPNPRMH"
     gene            3578..4507
                     /locus_tag="VNU48_07320"
     CDS             3578..4507
                     /locus_tag="VNU48_07320"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWI11122.1"
                     /translation="MNTPSTCAPAPPRPGIESLGVSREPGYPLPVGREPLALSTIHDL
                     PALPAALLELLRVLGNDSVETGTLAAKISHDQALTAKTLRLANSSFYGVSRHVNSVTD
                     AIAVLGLRAVRTLVTTAGLAGSFKPPTCEGFDFPGFWRHAIESAVGAKLVANAIGADG
                     ETAFTAGLLHDIGQLALASGFAAKYARVLALQAEHGIELCDAERALLGIDHPALGALL
                     AERWHFPGQIVEAIGAHHARALPGDTVVLADIVCVADTLAGLLGLGARNADALPIDCR
                     TVWAGAGLSAGSWEQILAQTGAQAQAICAALLP"
     gene            4516..9636
                     /locus_tag="VNU48_07325"
     CDS             4516..9636
                     /locus_tag="VNU48_07325"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWI11123.1"
                     /translation="MSAASIAYQEPAAAPAFVDPGGVTLRDALPAGALALPEVLDIGL
                     QLARSLADVHRLGVSHRHINPSNIVIEAPGLTSVRPEGPSRQARLIGFGPASTLADQQ
                     RADAQLEMAGSLAYLAPEQTGRTGRSVDQRADLYGLGATLYELVTGRAPFEDSDPLQL
                     IHKVLAQPPARPSGIPCALSDIVMRLLEKEPGRRYQSAAGLAHDLARLRDRLAAGELG
                     AFVLGEHDFAARLPPPSRPIGRDAEIAVLTATFADAVQGQGHTRGVLVAGVSGVGKTM
                     LINELRPLATARRGWFVMGTFDPHRQDLASDAVYQAFRALGKLLLAEPEAELVVLRER
                     MRLALGPNAGLIAALQPEFALLLGIDPRAQPPSATEEDPLQAERRLLQAAVDLLRGIA
                     SPQRPLVIALDDLQWAAALPLAFIDAVLTGDHLPGVLLVGACRTTEVDTAHPLTALRA
                     RWERLGVTLVRVALGNLPPADLGTLVGTMLRLAPAQAAPLAETLAERSAGNPFDTIEL
                     INALRHDGALLYGDGGWHWNAATIRRHIGSGNVVDLLSARIERLPHDTRAVLATLACL
                     GGEVGLGLLCTASDVTPETLEACVVPALDDGLLVPDPVGEGALRFCQPRVQQVAWERM
                     GPAGRQALQLSLARRLAPHHEFAAVAAEQYLGALEAVQDAPERRQVAQLFHRVATQAR
                     HTNGVLAERLLVGAIGLLAALNDAPDAASKAALAVDRHAVLYSLGRLEEADAVYASMA
                     FDPADPVQNSAIACIQVSSLSSSGRPKDAIALGLQWLAALGIAPLRGADFPADLSRRM
                     EGLCRWIDESSDAQDLQRPPLRDARIIAAARLISRMLPAAFTCDRAMMAWIGLESVRL
                     WVEHGPCAPLVANLGGIPAITVALRGDYRTGYLVARRALQRGEALGCELETTQVRMTL
                     ALLASHWFEPLEETKAQARLAFEALVRGGDLQFATIAQLALVRLQLDCGPTLSAVRSA
                     VDAAIAFAERTGDTHFGLCLPPYRELTRVLSGETGAQGADPTVDDQAAQAPGLASNPA
                     AMAQHHLMHALSAALFGDADRLSLHATAAMALLPALLATYASAFTYLLQALALADRVK
                     SAAAADRGALLAEFDACADWLRGRAADAPVNFSHLVKWVEAERAWACGEHYEATLAFD
                     AALREGAKRERPWHNALMTERAALFHLGQGLEHTGRELLSEARDLYAAWGATAKVQQL
                     DRRHAFLKTRSARRAGAGDARQSVSPDAIDMLGIVRASQALSSETSLERLRQRVVELL
                     AEMTGATSVLIVLWDDDMQQWLLPQAAPNDGGSGSGPTLAEAAAQGLLPLSVFRYAER
                     TREPLLVEDATRDDRFAGDAYLGSVEHCSLLVVPILHHGAARAMLLLENRLSSGAFTV
                     DRLDAVMLITGQLAVSLANAQLYEQLEQRVRERTRELQEAQAQLLAAARLAGMAEIAT
                     NVLHNVGNVLNSVNVSTGLIGARLRSSKVKGLARAVQMMDQHAGDLGTFLMHDAKGKL
                     LPSYLRELAPALEAEHEAMAAELGVLGESVDHIKEVVATQQSYAGAPRLVEMVVFSDL
                     LDDALRMNAGALTRHHVDVVKRLDELPEMPLDRHRLLQILVNLIGNAKQAMRADEPHP
                     CITLSAALTPHADGRVLRITVADNGEGIPPENLTRVFAHGFTTRKTGHGFGLHSCVLA
                     AQEMGGSLTAHSDGSGQGAAFILDIPVDIPVDIPESKP"
     gene            9633..>11116
                     /locus_tag="VNU48_07330"
     CDS             9633..>11116
                     /locus_tag="VNU48_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI11124.1"
                     /translation="MSAPSNRRILLVDDMPAIHADFRKILVPQGAVGAELGALEDALF
                     GQEEKAAVVAFELDSAYQGQEGLALVQASLAADRPYAMAFVDMRMPPGWDGVETIERL
                     WQADARLQVVICTAYSDTSWDDILARLDVRDRLLILKKPFDAVEVCQLANTLTAKWQM
                     TQNAAAQLTRLEDAVNERTRELTRANEALQSEIGEREHVQGQLAQSEKLASIGQLAAG
                     VAHEINNPIGYIFSNFGTLEKYLGDLFEMVAGYEAAEAQLAGTPIGAQLKSLRERCEL
                     DFLKEDIPTLMAESKEGITRVRTIVQGLKDFSRVDAHQEWGWANLNQGIDSTLNIVNN
                     EIKYKADVVKEYGVLPDVYCLPSELNQVFMNLLVNAAHAITVERGLITIRTGADAPAA
                     TVWIEVADNGCGIPKENLLRIFDPFFTTKPVGKGTGLGLSQVHGFAHQAGGTVKVTSE
                     LGKGTKIAILLPRKDVDLTVGNVSVTESGGSGTVLLVDDNPEVAA"
BASE COUNT         1778 a         3880 c         3793 g         1665 t
ORIGIN      
        1 gcttcggctt ccatggtctc tattgtcccc ttcctctcgt gtcgagaaca tcggaacagg
       61 gacagcgcga gcgtactacc tgtgatattc cgcaggcgtc gcaggcgcga cacgaccgcg
      121 ctgagatcat caacattgat gagaactcgg gcagcgcgca ggtcggccca tggcgtcgga
      181 cccgccgctc caggccttcg ttgacagcat cgagctgatc gacttgaagc gctcgacctc
      241 accgggacgc ttctgaagca gaaatcatca gtttgatcac ctatggattc gtcacaaaca
      301 cacacaagtc aatttagcgt gaacaaatgc ccgaagtttt cgatgggcga cggcgtgcgt
      361 atcactttca tcattaatga tcgctcagtg agagccgaca acagaagatg caacccgctc
      421 ccgcgacgac tccgacacct cgcatggccc gcttcgcttc ccgccacgac cccgccctgg
      481 tcctcgcctc gatcctgatg gcgctgccgg cctgtggcgt gctcggcctt ccggtcggga
      541 cgatcgagga ggcgcgatga gtcagcggct gaaccgacgc attctcctgg ccgacgacat
      601 gccggccatc catgcggact tccgcaaggt cctggcgccc acgacgccgg cgctgaacct
      661 ggacgaggtc gaatcggcgc tgttcggcac ggcggcccaa tcgactgccg tggcctttga
      721 attggacatt gcccaccagg ggcaggaggc actggcgaag gtccgcgagg cgctgcgcgt
      781 cggtcgcccc tacgcgctgg cgttcatcga catgcgcatg ccgccggggt gggacggggt
      841 cgagaccatc gagcagctct ggcgcgagga tccccggctg caggtggtga tctgcaccgc
      901 gtatgccgac cagtcctggg ccgaggtcgt cgatcgcctg gacgcgagcg accgtttgct
      961 cgtcctgaag aagcctttcg atccgatcga agtccgccag ctggccgctg ccctgacggc
     1021 gaagtggcag atgacggaag atgccaacgc cgaactgcag cgccgcgccg aactgctcgt
     1081 gcaggccgag gagatcgcgc tgatcggctc aatggagacc aatctcgtca acggcgagac
     1141 cacgctgtcg atcggcctgt gccacctgtt cggcgagccg gcgatcaccg cgccggtggc
     1201 accgacctgg ctttgtgcga gggtgcccga tgccgagcgc ccgttggtgc aatcgattcg
     1261 cgactcggtg cgaaccgacg agccgttcga gttccagcat cgcatcgtgc gacgcgacgg
     1321 cgtcatccgc acggtgctgc atcgctgccg cgtggaactc ggcgacgacg gcacgccgct
     1381 gcgtctgcat gcgacgctcc aggacatcac ctcgcggcgc gaggccgaac aagcgctcca
     1441 tgacctcgcg cacgccgacc tggcgacggg gctgccgaac cgccacgcgc tgctgaaccg
     1501 cctcagcgag gccacgctcg cggccgggcg cgaagatcgc ggcctcagtc tgctggtgat
     1561 ggagatcgac cggctcggtc tggcacgcga cagcctgggc cacgcgggcg gcgagcacct
     1621 gctgaaggcg gtggcgcagc ggctgctcaa gctggcatcc ggcgccggca tgatcgccca
     1681 cctcggcggc ggcgagtttg cactgctcgt ggatgcgtcc ggcgaagcgg ctgcgcagag
     1741 catggcgcgc gcgctggtgg acggcgtggc ccggccgttc catatcggca gcgccgagat
     1801 ctatgccagc gtcgccgtag gcgtcgcgtt gtggcccacg gacgcggacg ggcccgacga
     1861 actgctggac cgcgcctgcg cgtcggcgca gcgggcgcgc gagtcgggca gcaaccggat
     1921 ctgcttctac acgcccgaag cgaacgcgcg caccgcgacc aggctcgcca acgaaaccgg
     1981 cttgcggcgc gcgctcgacc gcaacgagct ctatctgagc taccagccgc aggtggatct
     2041 gggctcgggc cgagtgatcg gtctcgaagc gctggcgcgc tggaaggatg cgacgcgcgg
     2101 cgacatttcg ccggcggact tcatcccgct ggccgaggag acgggcctga tcgttccgat
     2161 cggcgaatgg gtgctgcgaa ccgcctgcat ggacagcatg cgctggcagg ccgagggcct
     2221 cgcgccggtg cgcgtcgcgg tcaacctgtc gatgctgcag cttcagcagc ccgacatcgt
     2281 gcagcggatt caggccatcc tgcaggaaac cggtctgaac ccccgccacc tcggcgtcga
     2341 agtcaccgag agcatggtga tggagcaggt cgagcatgtc gcgcagaccc tgtcgcagct
     2401 cagggccatc ggcgtcgaga tcgcgctgga cgactttggc accggttact cgaatctgag
     2461 ttacctgagc aagctgccga tcgacgtgct caagatcgac cgctccttcg tgcacgaggt
     2521 cactgccccc acgcaggacg tctcgatcac ccgcgcgctc atcaacatgg cgcacgggct
     2581 gaacatgaag gtgctggccg agggcgtcga gaacgaaggc cagctggccc tgctggtggc
     2641 cagtcgctgc gaccagatac agggctacta cttcagccgg ccggcgcgcg ccgacgcgat
     2701 cgccgcgatg ctgcgcgaag acaagcgcct gcccgaacac ctgctcgtgc gccggacgag
     2761 caagcgcacg ctgctgctcg tggacgacga ggacaacatc atcgcgtcgc tgcgccggct
     2821 gctgcgccgc gacggctacc acatcatcac cgcgaacagc ggcgcgcagg gtctgcagcg
     2881 gctcgcggag catgaggtcg atgtcatcct gtccgatcag cgcatgccgg gcatgaccgg
     2941 cgtggagttt ctgcgccgcg ccaaggagct gtacccgcag acggtgcgca tgacgctgtc
     3001 cggctacacc gagctgcagt cgattgccga tgccatcaac gagggcgcgg tctacaagtt
     3061 cctgaccaag ccttgggacg acgaacgtct gcgcgcgcac gtcaacgagg cctttcgcca
     3121 caaggagatc gccgacgaca accgcgagct cggtgcgcgt ctgcaggcgg cccacgacga
     3181 gctcgccacc gtcaatgcgc gtctgcacag gttgacggac atgcagctcc aacagattcg
     3241 ccgcgacgag gtgcggctgc tcagcgcaca cgagctgctg gagggcattc cgacgccggt
     3301 catcggcttc gacgtcgaag gcgtggtggc ctacctgaac gccgacgcgc acgagctgtt
     3361 cccgcccaac gactcgcccc tcggccgtgc ggcgacggag gttctgtcgc ccgaactgac
     3421 gcaagtctgg cacgccggcg acggtgccca ggtctgcgtg cggcttggcg cacttcactt
     3481 ccaggccgtg tgccgtccca tcggagacga gccgaaccag cgcggcaagt tgatggtcct
     3541 gtccccacac agccctaatc cgagaatgca ctgaatcatg aatacgccct ccacctgcgc
     3601 gccggcgccg cctcgccccg gcatcgaatc gctcggcgtt tcgcgcgagc ccgggtaccc
     3661 gctgcccgtc ggccgcgagc cgctggccct ctcgacgatc cacgacttgc ccgccctgcc
     3721 ggcggcgctg ctggaactgc tgcgcgtgct gggcaacgac agcgtcgaga ccgggacgct
     3781 cgcggccaag atctcgcacg atcaggcatt gactgccaag acgctgcggc tggccaattc
     3841 atcgttctac ggggtgtcgc gccacgtcaa ctcggtgacc gacgccatcg cggtgctggg
     3901 gctgcgcgcg gtccgcaccc tggtgaccac ggcagggctg gcgggcagtt tcaagccacc
     3961 cacctgcgag ggcttcgatt tcccgggatt ctggcgtcac gccatcgagt ccgcggtggg
     4021 cgccaagctc gtcgcgaatg cgatcggcgc cgacggcgag accgcgttca ccgccgggct
     4081 gctgcacgac atcgggcaac tcgcgctggc cagcggtttc gcagcgaagt acgcgcgggt
     4141 tctggcgctg caagccgagc acggcatcga gctgtgcgac gccgagcgcg cgctgctggg
     4201 catcgaccat ccggcgctcg gtgccctgct cgccgagcgc tggcactttc cgggccagat
     4261 cgtcgaggcg atcggcgccc accatgcacg cgcgctgccc ggcgacaccg tggtgctcgc
     4321 cgacatcgtc tgtgtggcgg acacgctcgc aggtctgctc gggctcggag cccgcaacgc
     4381 cgacgccctg ccgatcgact gcaggaccgt ctgggcaggc gcgggcctgt cggccggatc
     4441 ctgggagcag atcctggcgc agactggagc gcaggcccag gcgatctgcg ctgccctgct
     4501 gccatgagga ccgacatgag tgccgcttcg atcgcgtatc aagaacccgc cgccgcgccg
     4561 gcgttcgtcg acccgggcgg cgtgacgctg cgcgacgcat tgccggcggg ggcgctggca
     4621 ctgcccgagg tgctggacat cggcctgcag ttggcgcgca gcctggcgga cgtgcaccgg
     4681 ctgggcgtgt cccaccgcca catcaacccc tccaacatcg tgatcgaggc ccctggtctg
     4741 acgagtgttc ggccggaagg cccgtcccgg caggcgaggt tgatcggctt cggcccggcc
     4801 agcacgctgg ccgaccagca gcgcgctgac gcacagctcg agatggcggg ctcgctggcc
     4861 tatctggcgc ccgagcaaac cggccgcacc ggcaggtcgg tggaccagcg cgccgacctg
     4921 tacgggctcg gcgcgacgct gtacgagctc gtcaccggcc gcgcgccgtt cgaggacagc
     4981 gacccgctgc agctcatcca caaagtgctc gcgcagccgc ccgcgcggcc gtccgggatt
     5041 ccgtgtgcgc tgtccgacat cgtgatgcgc ctgctcgaga aggagcccgg gcggcgctac
     5101 cagagcgccg ccggcctggc gcacgacctc gcccggctgc gcgaccggct cgccgccggc
     5161 gagctcggcg ccttcgtgct cggcgagcac gacttcgccg cgcgcctgcc gccgccttcc
     5221 cggccgatcg gccgcgatgc cgagatcgcc gtgttgaccg ccacgttcgc cgatgccgtg
     5281 caaggccagg gccacacgcg cggcgtgctg gtggcaggtg tctcaggggt cggcaagacc
     5341 atgctgatca acgaactgcg cccgctcgcg acggcccggc gcggctggtt cgtgatgggc
     5401 acgttcgacc cgcaccgcca ggacctggcg agcgatgcgg tgtaccaggc ctttcgtgcg
     5461 ctcggcaagc tgctgctggc cgaacccgag gcggagctgg tggtgctgcg ggagaggatg
     5521 cggctggcgc tcgggccgaa cgccggactc atcgccgcgc tccagccgga gttcgcgctg
     5581 ctgctcggca tcgacccgcg agcccagccg ccatcggcca ccgaagaaga cccgctccag
     5641 gccgagcgcc gcctgctgca ggccgccgtg gacctgctgc gcggcattgc ctcgccgcag
     5701 cggccgctgg tgatcgcgct cgacgacctg caatgggccg ccgcgctgcc gctggccttc
     5761 atcgatgcgg tgttgaccgg cgaccacctg cccggcgtgc tgctcgtcgg tgcctgtcgc
     5821 acgaccgagg tcgacacggc gcaccccttg accgccctgc gcgcgcgctg ggaacgtctg
     5881 ggcgtgaccc tggtgcgggt cgcgctcggc aacctgccgc cagccgatct cggcacgctc
     5941 gtgggcacga tgctgcgctt ggcgccggcc caggctgcgc cgctggccga gacgctggcc
     6001 gaacgcagcg ccggcaaccc gttcgacacc atcgagctga tcaacgcctt gcgccacgac
     6061 ggcgccctgc tgtacggcga cggcggctgg cactggaatg ccgcgacgat ccgccgccac
     6121 atcggcagcg gcaacgtggt cgacctgctg agcgcgcgca tcgagcgctt gccgcacgac
     6181 acacgcgcgg tgctggccac cctggcttgc ctgggcggcg aggtcgggct cggcctgctg
     6241 tgcaccgcca gcgacgtgac gcccgagacc ctggaggcct gcgttgtgcc cgccctggac
     6301 gacggcctgc tggtgccgga tccggtcggc gagggggcat tgcgcttttg ccagccgcgc
     6361 gtgcagcagg tggcctggga gcgcatgggt ccggccgggc gccaggcctt gcaactgtcg
     6421 ctggcgcgcc ggctggcgcc gcaccacgag ttcgccgcgg tcgccgcgga gcaatacctg
     6481 ggcgcgctcg aagcggtgca agacgcgccc gagcgccgcc aggtggcgca actgttccac
     6541 cgcgtcgcga cgcaggcgcg ccacaccaac ggcgtgctgg ccgaacgttt gctggtcggt
     6601 gcgatcggct tgctcgcggc attgaacgat gcgcccgatg ccgcatcgaa ggcagcgctg
     6661 gcggtcgacc gccacgccgt gctgtattcg ttgggccgcc tcgaagaagc cgatgcggtc
     6721 tacgcatcga tggcgttcga ccccgctgat ccggtgcaga actcggccat cgcatgcatc
     6781 caggtcagca gcctgagcag cagcggccgc ccgaaggacg ccatcgcgct gggcctgcag
     6841 tggctggcag cgctgggcat cgcgccgctg cgaggtgccg acttccctgc cgacctgagc
     6901 cggcgcatgg aggggctgtg ccgctggatc gacgagtcga gcgatgcgca ggatctgcag
     6961 cggccgccgc tgcgagacgc gcggatcatc gcggcggcca ggctgatcag ccgaatgctc
     7021 ccggccgcct tcacgtgcga ccgggccatg atggcctgga tcgggctcga gagcgtccgg
     7081 ctgtgggtcg aacacggacc ttgtgctccg ctggtggcca acctcggcgg catcccggcc
     7141 atcaccgtcg ccctgcgcgg cgactaccgc accggctacc tcgtggcacg acgcgcgttg
     7201 cagcgcggcg aggcgctcgg ctgcgagctc gagaccacgc aggtccgcat gaccctggcg
     7261 ctccttgcat cgcattggtt cgaaccgctg gaggaaacca aggctcaggc gcggctcgcg
     7321 ttcgaggccc tggtgcgcgg cggagacctg cagttcgcga ccattgcgca gctcgcgctc
     7381 gtcaggctgc aactcgactg cgggcccacg ctgagcgcgg ttcgcagcgc cgtggacgcg
     7441 gccattgcgt tcgccgaacg caccggcgac acgcacttcg gcctgtgcct gccgccctac
     7501 cgcgagctca cgcgggtgtt gtccggcgag accggggcgc aaggggccga cccgaccgtc
     7561 gacgaccagg ccgcgcaagc tcccggcctc gcgtcgaacc ccgctgcgat ggcgcagcac
     7621 cacctgatgc atgcgctgtc ggcggccctg ttcggcgatg cggaccggct gtccctgcat
     7681 gccacggcgg cgatggccct gctgccggcc ctgctggcga cctacgcctc tgcgtttacg
     7741 tacctgctgc aggcgctggc gctggccgac cgtgtgaagt cggcagcggc cgccgatcgg
     7801 ggcgcgctgc tcgccgaatt cgacgcttgt gccgactggc tgcgcggccg tgcggcagac
     7861 gcgccggtca acttttcgca cctcgtcaag tgggtcgagg ccgagcgcgc ctgggcctgc
     7921 ggtgagcact acgaggccac actagccttc gatgccgcac tgcgcgaagg cgcaaagcgg
     7981 gagcgcccgt ggcacaacgc gctgatgacc gagcgtgccg cgctcttcca cctgggccag
     8041 ggcctggagc ataccggccg cgaactgctg tccgaagcgc gcgacttgta cgcggcctgg
     8101 ggcgcgaccg ccaaggtgca gcaactcgac cgcaggcatg ccttcctgaa gactcggagc
     8161 gcgcgccgcg ccggtgccgg ggacgcgcgc cagagcgtgt cccccgacgc gatcgacatg
     8221 ctcggcatcg tgcgcgcctc gcaggcgctg agctccgaga ccagcctgga gcggctgcgg
     8281 cagcgcgtgg tcgagttgct ggccgagatg accggcgcca cctccgtgct gatcgtgctg
     8341 tgggacgacg acatgcagca gtggctgctg ccccaggcgg cgccgaacga cggcggcagc
     8401 ggatcgggcc cgacgctggc cgaagccgcc gcgcaagggc tcctgccgct gtcggtgttc
     8461 cgctatgccg agcgcacgcg cgagccgctg ctggtggaag acgcgacgcg cgacgatcgc
     8521 ttcgccggcg acgcctatct gggcagcgtc gagcactgct cgctgctcgt cgtgccgatc
     8581 ctgcatcacg gcgcggcgcg cgccatgctg ctgctcgaga accggctgtc gagcggcgcc
     8641 ttcaccgtcg accggctcga cgccgtgatg ctcatcaccg ggcagctcgc ggtgtcgctg
     8701 gccaatgcgc agctctacga acagctcgaa caacgggtgc gcgagcgcac ccgcgaactg
     8761 caggaagcgc aagcccagtt gctcgcggca gcgcgcctgg ccggcatggc cgagatcgcg
     8821 accaacgtgc tgcacaacgt cggcaacgtg ctcaacagcg tcaacgtttc gaccgggctg
     8881 atcggcgcgc ggctgcgcag ctcgaaggtc aaagggcttg cgcgtgcagt gcaaatgatg
     8941 gaccagcacg ccggcgacct gggcaccttc ctgatgcatg acgccaaggg caagctgctg
     9001 ccgagctacc tgcgcgagct ggcgccggcg ctcgaggccg agcacgaggc catggccgcg
     9061 gaactcggcg tgctcggcga gagcgtcgac cacatcaagg aagtcgtcgc gacccagcag
     9121 tcgtatgccg gtgcgccgcg cctggtcgaa atggtggtct tcagcgatct gctggacgac
     9181 gcgctgcgca tgaacgccgg tgcgctgacg cgccaccatg tggacgtcgt gaagcgcctg
     9241 gatgagctgc ccgagatgcc gctggaccgc caccgcctgc tgcagatcct ggtcaacctg
     9301 atcggcaacg ccaagcaggc gatgcgcgcg gacgagccgc acccgtgcat cacgctcagc
     9361 gctgccctca cgccacatgc ggatgggcgg gtgctgcgca tcacggtggc cgacaacggc
     9421 gaaggcattc cacccgagaa cctgacgcgc gtcttcgcgc acggcttcac gacgcgcaag
     9481 accggccacg gcttcggcct gcacagctgc gtgctggcgg cgcaggaaat gggcggcagc
     9541 ctgaccgcgc acagcgacgg ctcgggccaa ggcgccgcct tcatcctcga cattcccgtc
     9601 gacattcccg tcgacatccc ggaaagcaag ccatgagcgc cccatcgaac cgacgcatcc
     9661 tgctggtcga cgacatgccc gcgatccacg cggacttccg caagatcctc gtgccccagg
     9721 gcgccgtcgg cgccgagctc ggcgcgctcg aggatgcgct gttcggccag gaagagaagg
     9781 ccgccgtggt cgccttcgag ctcgactcgg cctaccaggg ccaggaaggc ctggccctgg
     9841 tgcaggcttc gctcgcggcg gaccggccct acgcgatggc cttcgtcgac atgcgcatgc
     9901 cgccgggctg ggacggcgtc gaaaccatcg aacgcctgtg gcaggccgat gcgcggctgc
     9961 aggtcgtgat ctgcaccgcc tactccgaca cctcgtggga cgacatcctg gcgcggctcg
    10021 acgtgcgcga ccggctgctg atcctgaaga agcccttcga cgcggtcgag gtctgccagc
    10081 tcgccaacac gctcaccgcc aagtggcaga tgacgcagaa cgcggcggcg cagctcacac
    10141 ggttggagga cgcggtgaac gaacgcacgc gtgagctcac gcgcgccaac gaggccttgc
    10201 aaagcgagat cggcgagcgc gagcacgtcc aggggcagct ggcccagtcg gagaagctcg
    10261 cctccatcgg ccagctggcg gccggcgtcg cacacgagat caacaacccg atcggctaca
    10321 tcttctcgaa cttcgggacg ctcgaaaaat acctcggcga cctgttcgag atggtcgccg
    10381 gctacgaagc ggccgaagcc cagctggcgg gcaccccgat cggtgcgcag ctgaagtcgc
    10441 tgcgcgaacg ctgcgagctc gacttcctga aggaagacat ccccacgctg atggccgagt
    10501 cgaaggaagg catcacgcgg gtgcgcacga tcgtgcaggg cctgaaggac ttctcgcgcg
    10561 tcgatgccca ccaggaatgg ggctgggcga acctgaacca gggcatcgac tcgaccttga
    10621 acatcgtcaa caacgagatc aagtacaagg ccgatgtggt gaaggaatac ggcgtgctgc
    10681 ccgatgtcta ctgcctgcct tcggagctga atcaggtctt catgaacctg ctcgtgaatg
    10741 cggcccacgc gatcacggtc gaacgcggcc tgatcacgat ccggaccggc gcggacgcgc
    10801 ccgccgcaac ggtctggatc gaggtggcgg acaacggctg cggcatcccc aaggaaaacc
    10861 tgctgcgcat cttcgacccc ttcttcacga ccaagccggt cggcaaggga acgggattgg
    10921 gcctttcgca ggttcacgga ttcgcgcatc aggccggcgg aacggtcaag gtgacaagcg
    10981 aacttggcaa gggcacgaag attgcgatcc tgctgcctcg aaaggatgtc gatctaacgg
    11041 tcggtaacgt cagcgtcacc gagtccggtg gaagtggcac ggtgctgctg gtcgacgaca
    11101 atccggaggt cgccgc
//
LOCUS       DATMJB010000337         2399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_38277, whole genome shotgun sequence.
ACCESSION   DATMJB010000337 DATMJB010000000
VERSION     DATMJB010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38277"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..632
                     /locus_tag="VNU48_07335"
     CDS             24..632
                     /locus_tag="VNU48_07335"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HWI11125.1"
                     /translation="MNFLLKLAPLATAALLAACASPSPAPVLLTLPPVLMSTPGSAMP
                     AAPAAAPLLAVRRVTIPEYLVARRVRFRADAATLAEWPNTYWAERIEIGVSREFVSAL
                     RQQLPGWAICETTCGDHVPALTLQLDLVPMDFVRGAQQLQAHAHLTLGDGAAAARVLR
                     VEERSYTLAATADTAQGHAQAIAALIQKVAADAAPMIRDARR"
     gene            complement(672..>2399)
                     /locus_tag="VNU48_07340"
     CDS             complement(672..>2399)
                     /locus_tag="VNU48_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI11126.1"
                     /translation="DLSADEASQVRVNIEGTRNAVEFAKAIDAGHLHHVSSIAAAGMY
                     EGVFREDMFDEAEGLDHPYFMTKHESEKIVRKEAKLPWTVYRPALVVGDSTTGEMDKI
                     DGPYYFFKLIQRMRQILPPWMPGVGLEGGRINIVPVDFVVAALDHISHSKADLDKKCF
                     HLVDPNGYRVGDVLDIFSKAAHAPKMNLFINAALLGFIPKSVKKGMMALAPVRRVRHA
                     VMKDLGLPDDMLTFINFPTRYDCRETVAALKGSGIECPNLRDYAWRLWDYWERHLDPA
                     LLIDHSLRGTVNGKVVLVTGGSSGIGLAAAVKFAKAGAITVICARDEAKLALAKNEIL
                     AEAGKDATVVTYSADIANEENCKAFVAQLTADHGGVDFLINNAGRSIRRAIEASYERF
                     HDYERTMQLNYFGSLRITMGLLPGMVAKRRGHIVNISSISVLVNAPRFSAYVASKAAL
                     DAWTACAASEFQDQGITFTTVNMPLVRTPMTAPTKIYDSMPLLSTDEASDLIVQAVVD
                     KPVRVATRLGVFVELLHDVVPRVTQVLMNTTFRMFPDSAAAKGEKSGKASLSPEAMAM
                     AEMMRGIHF"
BASE COUNT          349 a          822 c          820 g          408 t
ORIGIN      
        1 gtcatcttcg ggaacaaacg cgaatgaact tcctgctcaa actcgcgcca ctcgccacgg
       61 cggccctgct cgccgcgtgc gcgtcgccgt cgcccgcgcc ggtcctgttg acgctgccgc
      121 cggtgctgat gtcgacgccg ggcagcgcga tgcccgcggc gcccgccgcc gcgccgctgc
      181 tcgcggtgcg ccgcgtcacc atccccgagt acctggtcgc gcgccgcgtg cgcttccgcg
      241 ccgacgccgc gacgctggcc gaatggccca acacctactg ggccgagcgc atcgagatcg
      301 gcgtgtcgcg cgaattcgtc tcggcgctgc gccagcagtt gcccggctgg gcgatctgcg
      361 agaccacttg cggcgaccac gtgccggcgc tgaccctgca actcgatctg gtgccgatgg
      421 atttcgtgcg cggcgcgcag cagttgcagg cgcatgcgca tctgacactc ggcgacggcg
      481 ccgccgcggc acgcgtgctg cgcgtcgagg agcgcagcta tacgctggcg gccaccgccg
      541 acacggccca gggccacgcg caggcgatcg ccgccttgat ccagaaggtc gccgccgacg
      601 ccgcgccgat gatccgcgac gcccggcgtt aggcgccacg cggcacgcgg cgcgcggcgg
      661 cgccgggccg gtcagaagtg gatgccgcgc atcatctcgg ccatcgccat cgcctccggc
      721 gacaagctcg ccttgccgct cttctcgccc ttcgccgccg cgctgtcggg gaacatgcgg
      781 aaggtcgtgt tcatcagcac ctgggtgacg cgcgggacga cgtcgtgcag cagctcgacg
      841 aagacgccga gccgcgtcgc gacacgaacc ggcttgtcga ccacggcctg cacgatcagg
      901 tccgaggcct cgtcggtcga cagcagcggc atgctgtcgt agatcttggt cggcgccgtc
      961 atcggcgtgc gcaccagcgg catgttgacg gtggtgaagg tgatgccctg gtcctggaac
     1021 tcgctggcgg cgcaggccgt ccaggcgtcg agcgcggcct tcgacgcgac gtaggccgag
     1081 aagcgcggcg cgttcaccag cacgctgatg gagctgatgt tgacgatgtg gccgcggcgc
     1141 ttggcgacca tgccgggcag caggcccatc gtgatgcgca gcgagccgaa gtagttcagc
     1201 tgcatcgtgc gctcgtagtc gtggaagcgc tcgtagctcg cttcgatcgc gcgccggatc
     1261 gaacgcccgg cgttgttgat caggaagtcg accccgccgt ggtcggccgt gagttgggcg
     1321 acgaaggcct tgcagttctc ctcgttcgcg atgtcggccg agtaggtgac gacggtcgcg
     1381 tccttgccgg cctcggccag gatctcgttc ttggccagcg ccagcttggc ctcgtcgcgc
     1441 gcgcagatca cggtgatcgc gccggccttc gcgaacttca ccgccgccgc cagaccgatg
     1501 cccgacgaac cgccggtgac cagcacgacc ttgccgttga ccgtgccgcg caacgagtga
     1561 tcgatcagca gcgccgggtc gaggtggcgt tcccagtagt cccacagccg ccaggcgtag
     1621 tcgcgcaggt tcgggcattc gatgcccgag cccttcagcg cggccaccgt ctcgcggcag
     1681 tcgtagcggg tcgggaagtt gatgaaggtc agcatgtcgt cgggcagccc gaggtccttc
     1741 atgacggcgt ggcgcacgcg gcgcaccggc gccagcgcca tcatcccttt cttgacgctc
     1801 ttcgggatga agccgagcag cgcggcgttg atgaacaggt tcatcttcgg cgcgtgcgcg
     1861 gccttgctga aaatgtcgag cacgtcgccg acgcggtagc cgttcgggtc gaccaggtgg
     1921 aagcacttct tgtcgagatc ggccttcgag tggctgatgt gatcgagcgc ggcgacgacg
     1981 aaatccaccg gcacgatgtt gatgcgcccg ccttcgaggc cgacgccggg catccacggc
     2041 ggcaggatct ggcgcatgcg ctggatcagc ttgaagaagt agtatgggcc gtcgatcttg
     2101 tccatctcgc cggtggtcga gtcgccgacg accagcgcgg ggcggtagac cgtccacggc
     2161 agcttggctt ctttgcgcac gatcttctcg ctctcgtgct tggtcatgaa gtacgggtgg
     2221 tccaggccct cggcctcgtc gaacatgtct tcgcggaaca cgccttcgta catgccggcg
     2281 gccgcgatcg aactgacgtg gtgcagatgc ccggcatcga ttgccttcgc gaactcgacc
     2341 gcgttgcgcg tgccttcgat gttcacgcgg acctgcgacg cctcatcggc cgacaggtc
//
LOCUS       DATMJB010000338         2962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_237217, whole genome shotgun sequence.
ACCESSION   DATMJB010000338 DATMJB010000000
VERSION     DATMJB010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2962
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237217"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1780)
                     /locus_tag="VNU48_07345"
     CDS             complement(<1..1780)
                     /locus_tag="VNU48_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HWI11127.1"
                     /translation="MSMPAIERVIPLVGQLPQSIRDALARRLRELAGLVLLALSGVGA
                     AALMTWSVQDPSLSHATSRAIRNIVGYPGAIGSDLLMQILGLGAIMLILPVAVWGWRL
                     LTHRSFDREALRIACWVLCTVIAAGFASCWPHGGSWPLPTGLGGVVGDALVRAPAVVF
                     GPPGFFYRFVLGVMLFAGMVAAFLFATGMGSRPQEEPLTQIEDDDTPFVEEKDRGSVS
                     LGWLFHALMSAKARLGWLLTAAYRSLVASAPQPLTSSFERQEPNLGGRAAPSLAPQDE
                     GDFDDEVEDEEEAPAARTPRKKAAARTPARKSSDKFELPSVSVLTAPKASDRQPLSKS
                     ELESNSRALEVVLSDFGVRGEIVKANPGPVVTLYELEPAPGIKSSRVIGLADDIARSM
                     SALSARVAVVAGRNAIGIELPNAHREKVYLRELLSVKDESVAKLPLCLGKNIGGESII
                     IDLARTPHMLIAGTTGSGKSVAINTMILSLVYRLRPDQCRLIMVDPKMLELSVYDGIP
                     HLLTPVVTDPKKAVVALKWAVREMEDRYRKMSKLGVRNLEGYNARVAEACAKGETLTR
                     TVHTGYDKETGEAIYEEETLDLEPLPF"
     gene            complement(1794..>2962)
                     /locus_tag="VNU48_07350"
     CDS             complement(1794..>2962)
                     /locus_tag="VNU48_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWI11128.1"
                     /translation="QAERSPFARTAELLASYHPTKPLITLSLGEPQHPIPAFVGPVLA
                     NRVADFGRYPIAKGIEPFRRAASNWLSSRFDLPRPIDPETEILVLNGSREGLFFAAIT
                     AARYVGPRKGAPAILLPNPFYPAYGAGSRAAGCETIYLPTTLANGFLPDLDALDDATL
                     ARTAAIFIASPANPQGAVASKQYFGRLKQLADRFGFMILSDECYSEIYTKQAPGSALE
                     SAGPDFANVVAFQSLSKRSNLPGMRVGFAAGDSKFLAAFHELRNVAAPQVPVPLQHVA
                     VAAYSDEKHVEENRRLYRIKFDLADQILGNRYGYVRPAGGFCLWLDVSAHGGDEAAAV
                     RLYRDAGVRVIPGSYLARQQEDGSNPGAGYIRLALVADSETTAEALHRLVEILD"
BASE COUNT          534 a          953 c          981 g          494 t
ORIGIN      
        1 ggaacggcag cggctcgaga tcgagcgtct cctcctcgta gatcgcctcg cccgtttcct
       61 tgtcgtagcc ggtgtgcacc gtccgcgtga gcgtctcgcc tttcgcgcac gcttcggcga
      121 cccgcgcgtt gtagccctcg agattgcgca cgccgagctt cgacatcttg cggtaacggt
      181 cctccatctc gcgcaccgcc catttcagcg cgaccaccgc cttcttcgga tcggtcacga
      241 ccggtgtcag cagatgcgga atgccgtcat agacggagag ctcaagcatt ttcggatcga
      301 ccatgatcag ccggcactga tcgggccgca gccggtagac caggctgagg atcatggtgt
      361 tgatggcgac cgatttgccg gagccggtgg tgccggcgat cagcatatgc ggcgtgcgcg
      421 caagatcgat gatgatggat tcgccgccga tattcttgcc gaggcacagt ggcagtttgg
      481 cgacggattc gtctttcacc gaaagcaact cgcgcaggta aaccttctca cgatgcgcat
      541 tcggcagctc gatgccgatg gcgttgcggc cggcgacgac ggcgacgcgc gccgagagcg
      601 cgctcatcga gcgggcgata tcgtcggcaa ggccgatgac gcgcgaggat ttgatgcccg
      661 gcgcgggctc gagttcgtac agcgtcacca ccgggcccgg attggccttg acgatttcgc
      721 cgcggacgcc gaagtcgctg agtaccactt ccagcgcccg cgaattcgat tccagctcgc
      781 tcttgctcag cggctgacga tccgaagcct ttggcgccgt cagcaccgat accgagggca
      841 gttcgaactt gtcggacgat ttgcgcgcag gcgtgcgggc agcggccttc ttgcgcggag
      901 tacgggctgc gggcgcttcc tcttcgtctt cgacttcgtc gtcgaaatct ccttcgtcct
      961 gcggggccag tgacggcgcg gcgcggccgc cgagatttgg ctcctgccgt tcgaacgagg
     1021 aggtgagggg ctgcggcgcg ctcgcgacca gcgaacggta ggcggcggtc aacagccagc
     1081 ccaaccgggc cttcgcgctc atcagcgcgt ggaacagcca tccgagcgaa accgagccgc
     1141 ggtctttctc ctccacaaac ggcgtgtcgt cgtcttcgat ctgcgtcaac ggctcttcct
     1201 gcggacgcga gcccatgccg gtggcgaaca ggaatgccgc gaccatcccg gcgaacagca
     1261 tcaccccgag cacgaagcga tagaagaagc cgggtggacc gaacaccacc gccggcgcgc
     1321 gcaccagcgc gtcgccgacc acgccgccaa ggccggtcgg cagcggccat gaaccgccat
     1381 ggggccagca gctggcaaag cccgctgcga ttaccgtgca gagaacccag cacgcgatac
     1441 gaagcgcttc acggtcgaag ctgcgatggg tcagcagccg ccagccccac accgccaccg
     1501 ggaggatcag catgatggcg ccgaggccga gaatctgcat caggaggtcg gagccgatcg
     1561 cgccgggata gccgacgatg ttgcggatcg cccgcgacgt cgcgtgactg aggctggggt
     1621 cctgcaccga ccacgtcatc agcgccgcgg cgccgacacc ggaaagcgcc agcagcacga
     1681 ggcctgccag ttcgcgcagc cttcgcgcca gcgcgtcgcg gatcgactgc ggcaactggc
     1741 cgaccagggg aatgacacgt tcgatcgctg gcatgctcat gggggccctg cgactaatcc
     1801 agaatttcga ccagccggtg caacgcctcg gctgttgttt cactgtccgc gaccagcgcg
     1861 aggcgaatgt agccggcgcc gggattgctg ccatcttcct gctgccgcgc cagataactt
     1921 ccgggaatca cgcgcacgcc ggcgtccctg tagagccgca ccgccgccgc ctcgtcgccg
     1981 ccatgggccg agacgtcgag ccagaggcag aagccgccgg cgggcctgac atagccatag
     2041 cgattgccaa ggatctggtc ggcgagatcg aacttaatgc ggtagagccg ccggttttct
     2101 tcgacatgct tctcgtcgct ataggccgcg accgcgacat gctgcaacgg caccggcacc
     2161 tgcggtgcgg cgacattgcg cagctcgtga aaggcggcga gaaatttgct gtcgccggcg
     2221 gcaaagccga cccgcatgcc cggcaggttc gaacgttttg acagcgactg aaacgccacg
     2281 acattggcaa aatcgggccc ggccgattcc agcgcgctgc ccggcgcttg cttggtgtag
     2341 atttccgaat agcactcgtc gctcaagatc atgaatccga agcggtcggc cagctgtttc
     2401 agccggccaa aatattgctt cgaggccacc gcgccttggg ggttagctgg ggaggcaatg
     2461 aagatcgcgg cggttcgcgc cagcgtggcg tcatcgagcg catccagatc cggcaggaaa
     2521 ccgttggcga gcgtggtcgg caggtaaatc gtctcgcagc ccgcggcgcg ggagcccgcg
     2581 ccataggccg gatagaaggg gttgggcagc aggatcgcgg gcgcgccctt gcgcgggccg
     2641 acataccgcg cggccgtgat cgctgcgaaa aacaggccct cgcggctgcc gttcagcacc
     2701 agaatctcgg tttcggggtc gatcgggcgc ggcaggtcga agcggctgga aagccaattc
     2761 gaggccgcgc gccgaaacgg ctcaatgccc ttggcaatgg ggtagcggcc gaaatcggca
     2821 acgcggtttg ccagtaccgg gccgacgaac gccggtatgg gatgctgcgg ttcgcctaat
     2881 gatagtgtaa tcaatggctt agtaggatgg taggaggcca gcagctcggc cgtccgcgca
     2941 aatggcgagc gttcggcttg ac
//
LOCUS       DATMJB010000339        10136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_739204, whole genome shotgun sequence.
ACCESSION   DATMJB010000339 DATMJB010000000
VERSION     DATMJB010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10136
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739204"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /locus_tag="VNU48_07355"
     CDS             <1..1177
                     /locus_tag="VNU48_07355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWI11129.1"
                     /translation="KVSHSFIRREILALERQGVAVLRVAVRGWADALADAADEAERTR
                     TRYLLQRGLAPPLLAAARLALTRPGRFVAAARLALSMWRRSQRTLFHHLAYLAEAAQL
                     VGWLAAERIDHLHAHFGTNPAEVAMLARVLGGPPYSFTVHGPEEFDHPEALGLGEKGR
                     RAAFVVAISSFGRSQLYRWLPQAHWHKVQVVHCGLERAFHEGVTSGVATARRLVCVGR
                     LCEQKGQLLLVQALQRVLARGVALELVLAGDGELRDEIEALVDRLGVRAQVRITGWIG
                     SAEVRDEIVAARALVLPSFAEGLPVVIMEAMALRRPVITTFVAGIPELVRDAENGWLV
                     PAGDIDALARAIEACLAAPPEVLERMGAAAHRCVLERHDIDREATKLAALFAAGPAP"
     gene            1233..2447
                     /locus_tag="VNU48_07360"
     CDS             1233..2447
                     /locus_tag="VNU48_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWI11130.1"
                     /translation="MSLCDLVGPVLLVFTLLMAIPAGVLVLQLVAALTKRAAPGEAPV
                     TPRDDTLTVLMPAHDEAAGIAAAIAAVCAQLGPRDRLLVVADNCSDATAAIARAAGAE
                     VTERADAQRRGKGYALDHGVRWLENDPRDPPGAVVIVDADCIVAPQALARLAQRCLSS
                     ARPVQALYLMHAPPRAPLGLRIAEFAWIVRNKVRPLGAAALGWPCQLMGTGMAFPWAL
                     IRGAPLATGHLVEDMQLGLDLAAAGAAPLFCPQALVSSVFPSDTAGARAQRTRWEHGH
                     LSVIARTGPRLLLRALARRDAASVALALDLMVPPLAALVLALGALLAIDLAWWWFAAD
                     ARPAGVAAVALALLAAAVLAAWRREARHIVTGRELLGLPLYVAAKIPVYVRLFTKRQV
                     EWVRTKRDDRSH"
     gene            2431..3585
                     /locus_tag="VNU48_07365"
     CDS             2431..3585
                     /locus_tag="VNU48_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HWI11131.1"
                     /translation="MTAATDSRVAAAIDAPPRRWVDFERRLVCILGLPFDAIDVAQAV
                     QRIRDAAFSGSRCFVSTPNLNFAITARSDAAFRDSVLRSDLSLIDGMPLVWIARLLGL
                     PVRGRVSGADVFEALQAHAGPPLTVYLFGGPPGAAARACEGINRRGGGVRCVGHDTAG
                     FGPIESMSGDAQIARINRSGAQFVVVALGAAKGQAWIEHNAARLSAPVLSHLGAVVNF
                     AAGTVARAPRWMQSLGLEWLWRIKEEPSLWRRYWNDSVALMGLLLSCVLPDALEARRS
                     RAPDTRAPRLESTRTSRGHVLRLSGDWRDADGIVTLRAALARCAADQAAVSIDLAGVS
                     GIGDSAIALLLVARGWFDAHGGWALTGANAAVTTTLRRKLAAVTLLGDPR"
     gene            3582..4160
                     /locus_tag="VNU48_07370"
     CDS             3582..4160
                     /locus_tag="VNU48_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11132.1"
                     /translation="MNHLEARVRVTNAPVSAEVPDWSREAKVFWHWHPSRSLLASLRA
                     YERLRHATRPDRVLMRWLAVLRHRFWSAVTGAEIPLSCHIEGGLLLPHPNGIVVHPDA
                     RIGPNCLLMQQVTLGTTGTFLGAPTLAGHVDLGAGAKVLGKVRLGAHSVVGANAVVLC
                     DVPEGATAVGIPARILPPRASPLAAGETGAAA"
     gene            4169..5464
                     /locus_tag="VNU48_07375"
     CDS             4169..5464
                     /locus_tag="VNU48_07375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWI11133.1"
                     /translation="MRVLIAAEHASARFGGEAALPLHYFRVLRRRGVPVWLVTHARTR
                     AELTQLYPGDARILYIEDTAWHRAMWHIGRRLPAQVAYFTTGFLSRLSTQLAQRRLVR
                     GLVREQGITVVHQPMPVSPREPSMLFGFGVPVVIGPMNGGMDYPPAFRRNRGGVERVL
                     LGIGRASAALLNALMPGKRRAATLLVANERTRAALPAGVCTNVVELVENGVDLSLWQA
                     PPAREDAPDGAVVTFAFMGRLVDWKAVDLLLRAFAQARAQAPMRLLILGDGVERAGLE
                     TLAAQLGIAGSDAASAGTVHFAGWLAQAACAQRLREADCLVLPSLLECGGAVVLEAMS
                     MAKPVIATAWGGPLDYLDPACGVLVAPTSRSEIVDAFAVAMVAMAKSASGRTRLGEVG
                     LAKVQSEFDWELKVDRMLGVYREAMRRQAAAADTKRTIR"
     gene            5461..6624
                     /locus_tag="VNU48_07380"
     CDS             5461..6624
                     /locus_tag="VNU48_07380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWI11134.1"
                     /translation="MTSTNHPQATVRQPYKFGFVLNTAIGNLTRYLNLRKYAQREPDV
                     ELTWAPVSHYTAPDVPSRLRFLPAPLFMRARVLQQAWPLLRRLDDFDAVMIHLFEADV
                     LCALRGCLRGRPLRFSSTDEAPIVDRANYPLYPNELAKSRLRQRARLALDLWRVRHTD
                     YFLPFSHWGANILVRDCGAPAERVRALHVGMDLELWRWAPKPARPAGRRLRLLFVGGD
                     FVRKGGPLLLQVFQAHFQDSAELHLVTKQAPKLLPANVHVHADFEPNDERLRQLYAEM
                     DLMVVPTTADVGPLWVFLEAMAMGLPIIGTEVGASTEIVRNGETGFTIKVGDGEALRQ
                     AIQHVCDRPELGHEMGLRGRALIESEYNAAVNVPSIVRTMKDAVDRQRAGRAS"
     gene            6674..7510
                     /locus_tag="VNU48_07385"
     CDS             6674..7510
                     /locus_tag="VNU48_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11135.1"
                     /translation="MNLLRSIVLKALHSGRLRHLRHRLFLRTRARAPAIAIEHDGPVR
                     VLVLGVYMADRANTVDHLVEAFASTQQGLQVEQRWVALNGSSANAKVREVTALVATQP
                     APKFALLNRLLRPSDPKDFDFIMACDDDIYLPRGFLAAYLAYQRKFDFAVAQPSRAWH
                     SHFDHAFVLRRPWLQARQTRFVECGPLVSLRRDAARLLVPFDQQDEMWGIDLVWAEVI
                     ERHGLRMGIVDALSVDHSLRPQASTYDKTDEDAAMKSYLSKRSHIPMHEVFTVVKRYP
                     LR"
     gene            7607..7978
                     /locus_tag="VNU48_07390"
     CDS             7607..7978
                     /locus_tag="VNU48_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11136.1"
                     /translation="MKSLVLALSLFAANAHALIPVPSDQEAQRAHAQGSCGKLGPGSG
                     LKDGKGWIVSMLPGKHQFRVRGGGKVYLTVARQSVVDFDVGRPYYIVIEDAAPGAVLE
                     WKIPGSEWGPVSKYFLYPPQQ"
     gene            8098..8850
                     /locus_tag="VNU48_07395"
     CDS             8098..8850
                     /locus_tag="VNU48_07395"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI11137.1"
                     /translation="MYEVIYSYLARLKAGTGDETNGRHVVGPLVVELCGAKTAPRVLD
                     VGAGYGKDLLGVKTALPGASTAAVEGYPRAVAFLRQSGVEVASIDIERDALPFGAGSF
                     DVVMCNQVLEHTKELFWVVSELARVCKLGGHLMIGVPNLGSLHNRLALLAGRQPPAIH
                     VFGPHVRGFTVPGMRDFLEAGNTLRVERVLGGNFYPFAPSVSRPLARWFPGFAVSSFY
                     AVRKLSDTSFLDVLDTSRGAELVDTPYFRGAA"
     gene            complement(8881..9960)
                     /locus_tag="VNU48_07400"
     CDS             complement(8881..9960)
                     /locus_tag="VNU48_07400"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWI11138.1"
                     /translation="MSEQPKLSIGENNFDFLRFLFASLVVFSHSFPLATGSEDLEPLA
                     LFTHRQLTFGSLAVDCFFIISGFLISHSWVSRPKPWPFLQKRIRRIYPGFIAAVLLGA
                     FVITPLFSVVGSAGITFDFVLEFVAKTSRLLQATPGKAFQNNPASGTVNGSLWSISYE
                     FWCYIGVMACGALGLLRRPLLLGSAVVGAMLVSLAFAWFQLTPGGSWLGRVLGYPPFW
                     ARLLPYFLVGMAFYAYRDRVPLTAKGAILCAALFAVASWIPYAMIFVLPVVAAYLIFW
                     FAFLPLSPLNRFARYGDFSYGIYLYSFPLLQMIVFWYGTRMNPFVLFAVGWPLSTVAA
                     AMSWHLVERRWLRPNRSNRELAPQT"
BASE COUNT         1450 a         3410 c         3659 g         1617 t
ORIGIN      
        1 gaaggtgagc cacagcttca tccggcgcga gatcctcgcg ctggagcgac agggcgtcgc
       61 ggtgctgcgc gtcgcggtgc gcggctgggc cgatgcgctg gccgatgccg ccgacgaggc
      121 cgagcgcacg cgcacgcgct acctgttgca gcgtggcctc gcgccacccc tgctcgccgc
      181 ggcccggctc gcgttgacgc ggcccgggcg cttcgtcgcc gccgcgcgcc tggccctgtc
      241 gatgtggcgc cgctcgcagc gcacgctgtt ccaccacctg gcctatctgg ccgaggccgc
      301 ccagctcgtc ggctggctcg cggccgagcg catcgatcac ctgcacgcgc acttcggcac
      361 caacccggcc gaggtcgcga tgctggcccg cgtgctcggc gggccgccgt acagcttcac
      421 ggtgcacggc cccgaagagt tcgatcaccc cgaggcgctc gggctcggcg agaagggccg
      481 ccgtgccgcg ttcgtcgtcg cgatcagttc cttcggccgc agccagctgt accgctggtt
      541 gccgcaggcg cactggcaca aggtgcaggt cgtgcactgc gggctcgaac gcgcgtttca
      601 cgagggcgtg acgagcggtg tggccacggc gcgccggttg gtgtgcgtcg ggcggctgtg
      661 cgagcagaag ggtcagctgc tgctggtgca ggcgctgcag cgcgtgctgg cccgcggcgt
      721 cgcgctcgaa ctcgtgctgg cgggcgacgg cgaactgcgc gacgagatcg aggcgctggt
      781 cgatcggctc ggcgtgcgag cccaggtgcg catcaccggc tggatcggca gcgccgaggt
      841 gcgcgacgag atcgtggccg cgcgcgcact ggtgctgcca agctttgccg agggcctgcc
      901 agtggtgatc atggaggcga tggcgctgcg ccggccggtc atcacgacct tcgtggccgg
      961 cattcccgag ctggtgcgcg acgccgagaa cggctggctc gtgccggccg gcgacatcga
     1021 cgcgctggcc agggccatcg aggcctgtct cgcggcgccg cccgaggtgc tcgagcgcat
     1081 gggcgcggcg gctcatcggt gcgtgctgga acggcacgac atcgaccgcg aggcgacgaa
     1141 gctggcggcg ctgtttgcgg cagggccggc cccatgagcc gccgggccgt tcccaaggcg
     1201 aataccgaag tgcgcagcac ggaggttgcc tgatgagcct gtgcgatctg gtcggcccgg
     1261 tgttgctggt gttcacgttg ctgatggcga taccggccgg agtgttggtc ctgcagctcg
     1321 tcgcggccct gacgaagcgc gccgcgcccg gtgaggcacc cgtgacgccg cgcgacgaca
     1381 cgctcacggt gctgatgccg gcgcatgacg aggccgcggg cattgctgct gcgatcgccg
     1441 cggtgtgcgc gcagctcggt ccgcgcgatc ggctgctggt ggtcgccgac aactgcagcg
     1501 acgcgaccgc ggcgatcgcg cgcgccgccg gtgccgaggt caccgagcgc gccgacgcgc
     1561 aacgccgcgg caagggctac gcgctcgatc acggcgtgcg ctggctggag aacgatccac
     1621 gcgacccgcc cggcgcggtc gtgatcgtcg atgccgactg catcgtcgcg ccgcaggcgc
     1681 tggcccggtt ggcgcaacgc tgcctgagct cggcccgacc cgtgcaggcg ctgtacctga
     1741 tgcatgcacc gccccgcgca ccgctggggc tgcgcattgc cgagttcgcc tggatcgtga
     1801 ggaacaaggt ccgcccgctc ggcgctgcgg ccctgggctg gccttgtcag ctgatgggca
     1861 ccggcatggc atttccctgg gcgctgatcc gcggcgcgcc gctcgccacc ggccatctgg
     1921 tcgaggacat gcagctcggc ctggatctgg ccgccgccgg cgcggcgccg ctgttctgcc
     1981 cgcaggcgct ggtcagcagc gtgttcccga gcgacaccgc gggcgcgcga gcccagcgca
     2041 cgcgctggga gcacggccac ctgtcggtga tcgcgcgcac cggtccgcgc ctgctgctgc
     2101 gtgcgctggc gcggcgcgac gccgcgtcgg tggcgctggc gctggacctg atggtgccgc
     2161 cgctggccgc gctggtgctc gccctcggcg cgctgctcgc gatcgatctg gcgtggtggt
     2221 ggtttgccgc cgacgcgcgg ccggccggcg tggcggccgt cgcgctggcg ctgctggccg
     2281 cggccgtgct cgcggcctgg cggcgcgagg cccggcacat cgtcaccggg cgcgagctgc
     2341 tcggcctgcc gctgtacgtc gctgccaaaa tcccggtcta tgttcgactg ttcacgaagc
     2401 gccaggtcga atgggtgcgc accaaacgcg atgaccgcag ccactgactc gcgcgttgcc
     2461 gccgcgatcg acgcgccgcc tcggcgctgg gtcgacttcg agcgccggct ggtgtgcatc
     2521 ctgggcctgc cgttcgatgc gatcgatgtc gcgcaggcgg tgcaacgcat ccgcgacgcc
     2581 gcgttcagcg gcagccgctg cttcgtctcg acgccgaacc tgaatttcgc gatcacggcg
     2641 cgcagcgacg cagcattccg cgactcggtg ctgcgcagcg acctgagcct gatcgacggc
     2701 atgccgctgg tctggatcgc gcgcctgctg ggcctgccgg tgcgcgggcg ggtttcgggc
     2761 gccgacgtgt tcgaggcgct gcaggcgcac gccgggccgc cgctcacggt gtacctgttc
     2821 ggcggcccgc ccggtgcggc ggcgcgcgcc tgcgagggca tcaaccggcg cggcggcggt
     2881 gtgcgctgcg tcgggcacga caccgcgggc ttcgggccga tcgaatcgat gagcggcgac
     2941 gcgcagatcg cgcgcatcaa ccgcagcggc gcgcagttcg ttgtcgtggc gctcggcgcc
     3001 gcgaagggcc aggcctggat cgagcacaac gcggcccggc tgagcgcgcc ggtgctctcg
     3061 cacctcggcg cggtcgtcaa tttcgccgcg ggcacggtgg cacgggcgcc gcgatggatg
     3121 cagtcgctcg ggctcgaatg gctgtggcgc atcaaggaag agccgtcgct gtggcggcgc
     3181 tattggaacg acagcgtggc gctgatgggg ttgctgctga gctgcgtgct gcccgacgcc
     3241 ctcgaggcgc gccgttcgcg tgcgccggac acgcgcgcgc cgcgactcga atcgacgcgc
     3301 acatcgcggg gccacgtgct gcggctcagc ggtgactggc gcgacgccga cggcatcgtc
     3361 actctgcgcg cggcgctggc gcgttgcgcg gccgaccagg cggccgtcag catcgacctg
     3421 gccggcgtga gcggcatcgg cgacagcgcc atcgccttgc tgctcgtggc gcgcggctgg
     3481 ttcgacgcgc acggcggatg ggcgctcacc ggcgccaatg ctgcggtgac gacgacgctg
     3541 cgccgcaagc tcgcggccgt gacgctgctc ggagacccgc gatgaaccac ctcgaggcgc
     3601 gggtgcgcgt cacgaacgct ccggtgtcgg ccgaggtgcc ggactggtcg cgcgaggcca
     3661 aggtgttctg gcattggcac ccgtcgcgct ccctgctggc cagcctgcgc gcctatgaaa
     3721 ggctgcgtca tgccacgcgg ccggaccggg tgctgatgcg ctggctcgcc gtgctgcggc
     3781 accgcttctg gagtgccgtg accggggccg aaattccgct gtcctgccat atcgagggcg
     3841 gcctgctgct gccgcacccg aacggcatcg tcgttcatcc ggatgcgcgc atcgggccga
     3901 actgcctgct gatgcagcag gtgacgctgg gcaccaccgg tacctttctc ggcgcgccga
     3961 cgctcgccgg gcatgtcgac ctcggcgcgg gcgccaaggt gctcggcaag gtgaggctgg
     4021 gcgcgcacag cgtggtcggt gccaacgcgg tcgtgctgtg cgacgtgccc gagggcgcca
     4081 ccgcggtcgg tatcccggcg cgcatcctgc cgccgcgagc gtcgccgctt gccgccggcg
     4141 agacgggggc cgcggcttga ccgacgggat gcgcgtcctc atcgccgccg agcacgcctc
     4201 ggcgcgcttc ggcggcgagg ccgcgctgcc gctgcactac ttccgcgtgc tgcgccggcg
     4261 cggcgttccg gtgtggctgg tgacgcatgc gcgcacgcgc gccgagctca cgcagctcta
     4321 ccccggcgac gcgcgcatcc tgtacatcga ggacacggcc tggcatcgcg cgatgtggca
     4381 catcggccgg cggctgccgg cgcaggtcgc gtacttcacg accgggttcc tgtcgcggct
     4441 gtcgacgcag ctggcgcaac gccggctggt gcgcgggctg gtgcgcgagc agggcatcac
     4501 ggtcgtgcat cagccgatgc cggtgtcgcc gcgcgaaccg tcgatgctgt tcggcttcgg
     4561 cgtgccggtc gtgatcgggc cgatgaacgg cggcatggac tacccgcccg cattccgccg
     4621 caaccgcggt ggtgtcgaac gcgtgctgct cgggatcggt cgcgccagtg ccgcgctgct
     4681 gaacgcgttg atgcccggca agcgccgcgc cgcgacgctg ctggtggcga acgagcgcac
     4741 ccgcgccgcg ctgccggcgg gcgtgtgcac gaacgtggtc gagctggtgg agaacggcgt
     4801 cgacctcagt ctgtggcagg cgccgcccgc gcgcgaggac gcgcccgacg gtgcggtggt
     4861 gaccttcgcg ttcatgggcc ggctggtcga ctggaaggcc gtcgatctgc tgctgcgggc
     4921 atttgcgcag gcgcgcgcgc aggcgccgat gcgcctgctg atcctgggcg acggtgtcga
     4981 gcgggccggg ctcgaaacgc ttgccgcgca actgggaatc gcgggcagcg acgcggcttc
     5041 ggccggcacg gtccacttcg ccggctggct ggcgcaggcg gcgtgtgcgc agcgcctgcg
     5101 agaggccgac tgcctggtgc tgcccagcct gctcgaatgc ggcggtgccg tcgtgctcga
     5161 ggcgatgtcg atggccaagc cggtgatcgc caccgcgtgg ggcgggccgc tcgactacct
     5221 cgatccggcc tgcggcgtgc tggtggcgcc gacctcgcgc agcgagatcg tcgacgcgtt
     5281 cgccgtggcg atggtcgcga tggcaaagtc ggcatccggg cgcacgcgcc tgggcgaagt
     5341 cggcctggcg aaggtgcagt ccgaattcga ctgggaactc aaggtcgacc gcatgctggg
     5401 cgtctatcga gaggcgatgc ggcgccaggc ggccgcggcc gacaccaagc gaaccatccg
     5461 atgacttcca ccaaccaccc gcaagcgacc gtgcgccagc cgtacaagtt cggcttcgtg
     5521 ctgaacaccg cgatcggcaa cctgacccgc tatctgaacc tgcgcaagta cgcgcagcgc
     5581 gagccggatg tcgaactgac ctgggcgccg gtgagccact acaccgcgcc cgacgtgccc
     5641 agccgcttgc gcttcctgcc ggcgccgctg ttcatgcggg cccgcgtgct gcagcaggcg
     5701 tggccgctcc tgcggcgcct cgacgacttc gacgccgtga tgatccacct gttcgaggcc
     5761 gatgtgctgt gcgcgctgcg cggctgcctg cgtgggcggc cattgcggtt cagcagcacc
     5821 gacgaggccc cgatcgtcga ccgcgccaac tacccgctgt atccgaacga actcgccaag
     5881 tcgcgccttc ggcaacgcgc ccgcctggcc ctcgatctct ggcgcgtgcg gcataccgac
     5941 tacttcctgc cgttctcgca ttggggcgcc aacatcctgg tgcgggactg cggcgcgccc
     6001 gccgaacggg tccgggcgct gcacgtgggc atggacctcg agttgtggcg ttgggcgccg
     6061 aagcccgcac ggcccgcggg ccggcgcctg cgcctgctgt tcgtcggcgg cgacttcgtc
     6121 cgaaagggcg gcccgctgct gctgcaggtg tttcaggccc actttcagga cagcgccgaa
     6181 ctgcatctgg tcaccaagca ggccccgaag ctgctgccgg cgaatgtcca tgtgcacgcc
     6241 gacttcgagc ccaatgatga acggctgcgg cagctgtacg cggaaatgga cctgatggtc
     6301 gtgcccacga cggccgacgt cggcccgctc tgggttttcc tggaggcgat ggcgatgggc
     6361 ttgccgatca tcggcacaga ggtcggtgcg tcgaccgaga tcgtcaggaa cggcgagacc
     6421 gggttcacga tcaaggtggg cgacggcgag gcgctgcgcc aggcgatcca gcacgtttgc
     6481 gatcgacccg agctgggcca cgagatgggc ctgcgcggca gggcgttgat cgaaagcgag
     6541 tacaacgcgg ccgtcaacgt cccgtcgatc gttcgaacga tgaaggatgc ggtcgaccgc
     6601 cagcgggcgg gccgggcgtc gtgatcgttg cgatgggtcg accggtggcg tgggcccagg
     6661 gtcgactgtt ctcatgaatc tgcttcgctc gatcgttctg aaggcgcttc actcgggccg
     6721 cctgcggcac ctgcgccatc gtctgttcct gcgcacgcgt gcacgggcgc ccgcgatcgc
     6781 gatcgaacac gacggcccgg ttcgcgtcct cgtgctcggc gtctacatgg ccgacagagc
     6841 caacacggtc gaccacctcg tggaagcgtt cgcatcgacg cagcagggct tgcaggtcga
     6901 gcagcgctgg gtcgccttga acgggagctc ggcgaatgcg aaggttcgcg aggtcaccgc
     6961 gctggtcgcg acccagcccg cgcccaagtt cgcgctgctg aaccggctgc ttcgaccttc
     7021 ggacccgaag gacttcgact tcatcatggc ctgcgacgac gacatctacc tgccgcgagg
     7081 tttcctggcc gcctacctcg cctaccagcg caagttcgat ttcgccgtcg cccagccgtc
     7141 gcgcgcctgg cacagtcact tcgaccatgc gttcgtgctc aggcgcccat ggctgcaggc
     7201 ccgccagacc cggttcgtcg aatgcggccc gctggtctcg ctgcgacggg atgcggcccg
     7261 gttgctggtg ccgttcgacc agcaggacga gatgtggggc atcgatctgg tctgggccga
     7321 ggtgatcgag cgacacgggc tgaggatggg catcgtcgac gcgctgtcgg tcgatcacag
     7381 cctgcgcccg caggcatcga cctacgacaa gacagacgaa gacgcggcga tgaagagtta
     7441 cctttcgaag cgatcgcaca tcccgatgca cgaggtattc accgtcgtga agcgatatcc
     7501 tttgcgctag cgaagcgtcg caggcgatgc ggcttgctgg cgcgtgcaca cgacgcctca
     7561 ggaagccggc agatctgccg ataaccgcgc acagacacct cccgccatga aatccctggt
     7621 cctcgcgctg tcgctcttcg ccgccaatgc gcatgcgctc atcccggtgc cgtcggatca
     7681 ggaggcgcaa cgcgcgcacg cacagggatc ctgcggcaag ctcggccccg gctcgggcct
     7741 gaaggacggc aagggctgga tcgtttcgat gctgccgggc aagcaccagt tccgcgtgcg
     7801 cggcggcggc aaggtctacc tgacggtggc caggcagagc gtcgtggact tcgacgtcgg
     7861 gcgcccctac tacatcgtga tcgaggatgc ggcgcccggc gcggtgctgg aatggaagat
     7921 ccccgggagc gaatgggggc cggtctccaa atacttcctc tatccgccgc aacagtgatc
     7981 tgccatggcg gtctcgcccc gcgcgccgac cttcgcgcga cacgccgtcg ttgatccggc
     8041 atcgaaggtg atgccgaccc ggccgggaga taagatccac aagacgatgg gcggacgatg
     8101 tacgaggtca tctacagcta cttggcgcgc ttgaaggctg gcaccggcga cgaaacgaat
     8161 ggtcgtcacg tcgtcggtcc gctggtcgtc gagttgtgtg gagcgaagac ggcgccccgt
     8221 gtgctcgatg tcggcgccgg ctatggcaag gatctgctcg gggtcaagac cgcactgccg
     8281 ggtgcgtcga ccgctgccgt cgagggctac ccgcgggcgg tggcgtttct tcggcagtcg
     8341 ggcgtcgagg tggccagtat cgacatcgaa cgcgatgccc tgccgtttgg cgccgggtcg
     8401 ttcgacgtcg tgatgtgcaa tcaggtgctc gagcacacca aggagttgtt ctgggtcgtg
     8461 tctgagcttg cgcgcgtgtg caagctcggt ggccacctga tgatcggcgt gccgaacctg
     8521 ggttcgcttc acaatcgact cgcgctgctg gcgggccggc agccaccggc gatccacgtt
     8581 ttcggcccgc acgttcgcgg attcaccgtt cccgggatgc gcgacttcct cgaggccggc
     8641 aacacccttc gcgtcgagcg ggtgctgggt ggcaacttct atcctttcgc gccgtcggtc
     8701 tctcgcccct tggcgcgttg gttccccggg tttgcggtgt cgtcgttcta cgcggtgcgc
     8761 aagttgagcg acaccagctt cctcgacgtg ctcgatacat cgcgcggcgc cgaactcgtg
     8821 gacacgcctt acttccgcgg cgccgcgtag aggctgcccg ttcccggtgg gtcggatgct
     8881 tcaggtctgt ggggccagtt ctcggttcga cctgttcggt ctgagccaac ggcgttcgac
     8941 gaggtgccag ctcattgcgg ccgcgacggt ggacaggggc catccgacgg cgaagagcac
     9001 gaacgggttc atgcgtgtgc cgtaccagaa cacgatcatc tgcagcaggg gaaacgagta
     9061 gagatagatg ccgtacgaaa agtcgccgta ccgcgcgaac cggttcagcg gcgagagcgg
     9121 tagaaaggcg aaccagaaga tcaggtaggc ggccacgact ggaagcacga agatcatcgc
     9181 gtacggaatc catgaggcga ctgcgaacaa ggcggcgcac aggatcgctc ccttggccgt
     9241 cagcggcacg cgatcgcgat aggcatagaa ggccatgccc accaggaagt acggcagcag
     9301 acgcgcccag aacggcgggt acccgagaac ccttcccagc caactgcccc cgggggtcag
     9361 ttggaaccag gcaaacgcca ggctgacgag catcgcgccg acgaccgcgc ttcccagcag
     9421 cagcggtctt cgaagcaagc cgagggcgcc gcaggccatg acgccgatgt agcaccagaa
     9481 ctcgtacgag atcgaccaca gtgatccgtt gacggtgccc gatgctggat tgttctggaa
     9541 cgctttgccc ggtgtggcct gcagcagcct cgaggtcttg gcgacgaact cgagcacgaa
     9601 gtcgaacgtg atgccggccg aaccgacgac cgagaacagc ggcgtgatga cgaaggcacc
     9661 gagcagcact gcggcgatga agcccgggta gatgcgtcga atgcgctttt gcaggaaagg
     9721 ccagggcttt ggccggctga cccaactgtg cgagatgagg aagccgctga tgatgaagaa
     9781 gcagtcgacc gccaggctgc cgaaggtcag ctgccggtgg gtgaacagcg ccagaggttc
     9841 caggtcctcg ctcccggtcg cgagggggaa gctgtgagag aagacgacga gcgaggcgaa
     9901 caggaacctg aggaagtcga agttgttctc gccaatgctc agcttgggct gctcgctcaa
     9961 ttcgttcgct ccagggttgc cgagcagggc ctgatggccc ggccgccagc tcggggcgag
    10021 cgactgcggg ctggcggtca ggacggctgc cgcatgtgtt gctcaggcta acacgcgtgg
    10081 ctgaagtccc tggagcatcg accttgtcag tgggtcaata cagggtgcgg ccatcc
//
LOCUS       DATMJB010000340         2032 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_839664, whole genome shotgun sequence.
ACCESSION   DATMJB010000340 DATMJB010000000
VERSION     DATMJB010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839664"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="VNU48_07405"
     CDS             <1..172
                     /locus_tag="VNU48_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HWI11139.1"
                     /translation="KIREHSLQKVPYILVVGDKEKANGAVAVRARGNQDLGVMPLADF
                     TQKIAADIAHKV"
     gene            277..825
                     /gene="infC"
                     /locus_tag="VNU48_07410"
     CDS             277..825
                     /gene="infC"
                     /locus_tag="VNU48_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWI11140.1"
                     /translation="MATFADRRTPNAERKHRLNREIMAPEVRLNGPENEPLGIVSITE
                     ALRLAGEADVDLVEIVAQADPPVCRLMDYGKFKYQEQKRAAEAKSKQKVIEVKEVKFR
                     PGTDEGDYAIKMRNLRRFIEEDGDKGKVTLRYRGREITHQDIGMRLLERIRDELADVS
                     VVEHMPKLEGRQMIMVLAPKRK"
     gene            947..1150
                     /gene="rpmI"
                     /locus_tag="VNU48_07415"
     CDS             947..1150
                     /gene="rpmI"
                     /locus_tag="VNU48_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWI11141.1"
                     /translation="MPKMKTKKSAAKRFRVRPGGTVKRGQAFKRHILTKKTTKNKRHL
                     RGTVAVHETNMGHMKQMLPFAGL"
     gene            1175..1531
                     /gene="rplT"
                     /locus_tag="VNU48_07420"
     CDS             1175..1531
                     /gene="rplT"
                     /locus_tag="VNU48_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWI11142.1"
                     /translation="MPRVKRGVTARARHKKVLALAKGYRGRRKNVFRIAKQAVMRAGQ
                     YAYRDRRTKKRVFRHLWIARINAGSRSHGVTYSKFMAGLKKLSIEIDRKVLSDMAIHD
                     PAAFASIVEKVKAQLI"
     gene            1662..>2032
                     /locus_tag="VNU48_07425"
     CDS             1662..>2032
                     /locus_tag="VNU48_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWI11143.1"
                     /translation="MNDLSPLVQEARDEFERAALPAELENAKARYLGKAGRITEQLKA
                     LGGMGPDDKKARGALINTAKQQIEAALNARREALAEAELQTQLKGEALDVTLPGRQRG
                     AGGGLHPISRARERIEAIFGS"
BASE COUNT          440 a          628 c          645 g          319 t
ORIGIN      
        1 taaaatccgc gagcattcct tgcaaaaggt cccgtacatc ctcgtcgtag gtgacaagga
       61 gaaggcaaac ggggctgttg cagtgcgcgc ccggggcaat caggacctcg gtgtgatgcc
      121 actggccgac ttcacccaga agatcgccgc tgacatcgcc cacaaagtgt gagagcactg
      181 gcgtcgtcgc ctcggtgacg gcgctcccgg tcgtcggggc gcgcgtttat ttttgttggg
      241 ctccgttgcc ttggggctcg ttgaaaggtc tggaccatcg ctacttttgc tgaccgccgt
      301 actcccaacg cagagcgcaa acaccgcctc aaccgggaga tcatggcgcc tgaagtccgt
      361 ctgaacgggc ccgagaacga accgctgggc atcgtgagca tcacagaggc gttgcgcctc
      421 gccggcgagg ccgatgtcga tctggtcgag atcgtcgcgc aggccgatcc accggtgtgc
      481 cggctgatgg actacggcaa gttcaagtac caggagcaaa agcgcgcggc cgaggccaag
      541 agcaagcaga aggtcattga agtcaaggaa gtgaagttcc ggcccggtac cgatgaaggc
      601 gactacgcga tcaagatgcg caacctgcgc cgcttcatcg aggaagacgg cgacaagggc
      661 aaggtcacgc tgcgctatcg cggccgcgag atcacccacc aggacatcgg catgcgcctg
      721 ctcgagcgca ttcgcgacga actggcggat gtctcggtgg tcgagcacat gcccaagctc
      781 gaaggccgcc agatgatcat ggtgctggcg ccgaaacgga agtaagagtc gtagagagtt
      841 cagcgtgccg gtgcgggttg ccggcgcgtt gccagaagcg actgcaggcc tacgagacgg
      901 caagagtttg ccggcgcctg caggagcaat caaaaaggag catcacatgc ccaaaatgaa
      961 gaccaagaaa agcgccgcga agcggtttcg cgttcgtcca ggcggcaccg tcaagcgggg
     1021 ccaggcgttc aagcgccaca tcctgaccaa gaagaccacg aagaacaagc gccacctgcg
     1081 tggcactgtc gccgtgcatg agaccaacat ggggcacatg aagcaaatgc tgcccttcgc
     1141 cggtctgtaa ccgcttcaag gaaaggatag aaacatgcct cgcgtcaaac gtggtgtcac
     1201 agcccgcgcc cgtcacaaga aagttctcgc actcgccaag ggttaccgcg gccgccgcaa
     1261 gaatgtattc cgcattgcca agcaagccgt catgcgtgcc gggcaatacg cctaccgtga
     1321 ccgccggacc aagaagcgcg tcttccgtca tctgtggatc gcccggatca atgccggcag
     1381 ccgcagccac ggcgtcacct acagcaagtt catggccggc ctgaagaagc tgtcgatcga
     1441 gatcgatcgc aaggttctgt cggacatggc gattcacgat ccggcagcgt ttgccagcat
     1501 cgtcgagaag gtgaaggccc agctcatctg atgtcgccac ggtgcagtgc tcgcgcgctg
     1561 tgcctcgcga caagtccggt cgatggcaac gtcgatcggg cgagcttttc aaggccgacc
     1621 tcgtgtgcgg cctttcttat ttgcgcagca gcagtaaatc gatgaacgac ctcagccctc
     1681 tggtgcaaga agcgcgagac gaattcgaac gggccgcgtt gcccgccgag ctcgagaacg
     1741 ccaaggcgcg ctacctcggc aaggctggcc ggatcaccga gcagctgaag gcgctgggcg
     1801 gcatgggccc ggacgacaag aaggcgcgcg gcgcactgat caacaccgcc aagcagcaga
     1861 tcgaggccgc cctgaacgcg cgccgcgagg cactcgccga agcggagctt caaacccagt
     1921 tgaagggcga ggccctcgac gtgaccttgc ccggccgcca gcgcggggcc ggcggcggcc
     1981 tgcacccgat ctcgcgcgcc cgcgagcgca tcgaggcgat cttcggctcg at
//
LOCUS       DATMJB010000341         2570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_841335, whole genome shotgun sequence.
ACCESSION   DATMJB010000341 DATMJB010000000
VERSION     DATMJB010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841335"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VNU48_07430"
     CDS             complement(<1..286)
                     /locus_tag="VNU48_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI11144.1"
                     /translation="MHYFVTGATGFIGKRLVKALLARRGATVYFLLRPESESKVAGLL
                     EYWGLSGASGHARAVPVLGDLTSKKLGVAADDVKRLKGKIDHVYHLAAVYD"
     gene            481..990
                     /locus_tag="VNU48_07435"
     CDS             481..990
                     /locus_tag="VNU48_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HWI11145.1"
                     /translation="MVKKLKQMAEKRAASGGRLFDSALAGQVKDSAQQIWLAGMGAFA
                     RAQAEGTKVFETLVKEGASLQKKTHSAAEEKLSEVTSKMTSMAGDVQAKAGQHWDKLE
                     SIFEERTAKALKKLGVPSAKDVDALMARIDELGAKVAKLSKAAPSGKPAPAKKAPAGR
                     RAAARKPAA"
     gene            complement(1033..>2570)
                     /locus_tag="VNU48_07440"
     CDS             complement(1033..>2570)
                     /locus_tag="VNU48_07440"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HWI11146.1"
                     /translation="CTDRQRPFEAMARQQAMNHTAYEELTGIWTRLHHFQHLQSIASW
                     DQAAMMPSKGNTARANAMAEMEGLLHRMRTDPNLAVLLMRAAGEDIDPFARANLGEIR
                     REWLSANALPRSLVEKLSLAGARCEHAWRTQRPANDWAGFLANFREVLALGREVAQRL
                     ADETGLAPYDALMDQYEPGTTSAEVDRVFGDLQQWLPGLVAQVRGRQATETVIEATGP
                     FPKATQRALSLDVMTLLGFDFEGGRLDESAHPFSGGVPEDTRLTTRYHEDDLLQSLMG
                     TIHETGHARYEQNLPRVWLGQPVAHARSMGIHESQSLAFEMQLGRSRAFAALLAPLLR
                     KHFGDQPAFEPDNLHRLMTRVAPGFIRVDADELTYPAHVILRYGIEKQLIAGSIAADD
                     IPALWDEGMQSLLGIDTRGNYRDGCLQDVHWTHGAFGYFPCYTLGAMYAAQWFAVIRR
                     LHPDLDARIARGDLAPAFDWLREHIWSQGSRWSTAELATRASGETLNPQHLRHHLEAR
                     YLG"
BASE COUNT          378 a          887 c          925 g          380 t
ORIGIN      
        1 ggtcgtagac cgccgccagg tggtagacgt ggtcgatctt gcccttcagg cgcttgacgt
       61 cgtcggcggc gacgccgagc ttcttcgagg tcaggtcgcc aagcaccggc accgcccggg
      121 catggccgct cgcacccgac aggccccagt actcgagcag ccccgcgacc ttgctctcgc
      181 tttctggccg cagcaggaag taaaccgtcg cgccgcgccg cgccaacaac gccttgacca
      241 ggcgcttgcc gatgaagccg gtcgcgccgg tgacgaagta gtgcatgggg ttcctccgag
      301 gggcgctgcg agctttgcgc gattcttgat caatcccctg ggcgcaccgc tgccgattta
      361 ccccaagcgg ggtagagccg gctcactagc gaaatgagat gcatggcgct tgcgccgctt
      421 ctatagtccg gcccacgcgc ggcgcggcgc cgcacccacc ccagcccgag gactcgcacg
      481 atggtcaaga aactcaagca gatggccgag aagcgggccg cctccggcgg ccgtctgttc
      541 gacagcgcgc tggccggtca ggtcaaggac tcggcgcagc agatctggct cgccggcatg
      601 ggcgcgttcg cgcgcgcgca ggccgagggc accaaggtgt tcgagacgct ggtgaaggaa
      661 ggcgcctcgc tgcagaagaa gacgcactcg gccgccgaag agaagctcag cgaagtcacc
      721 agcaagatga cgagcatggc cggcgacgtg caggccaagg ccggccagca ctgggacaag
      781 ctcgagagca tcttcgagga gcgcaccgcg aaggcgctga agaagctcgg cgtgccgtcg
      841 gccaaggacg tggacgcgct gatggcgcgc atcgacgagc tcggcgcgaa ggtcgcgaag
      901 ctgtcgaagg ccgcgccgtc gggcaagccc gcgccggcga agaaggcgcc ggccggcagg
      961 cgcgccgcgg cacgcaagcc cgcggcctga ggctcacgcg ccccgcctcg cggcgcggcg
     1021 cggcggcaaa gctcagccga ggtagcgcgc ctcgaggtgg tggcgcaggt gctgcgggtt
     1081 cagcgtctcg ccgctggcgc gcgtcgcgag ttccgcggtg ctccagcggc tgccctgcga
     1141 ccagatgtgc tcgcgcaacc agtcgaacgc cggcgccaga tcgccgcgtg cgatgcgcgc
     1201 gtcgaggtcc gggtgcaggc gccggatcac cgcgaaccac tgcgccgcgt acatggcgcc
     1261 gagcgtgtag caggggaagt agccgaaggc gccgtgggtc cagtgcacgt cctgcaggca
     1321 gccgtcgcgg tagttgccgc gcgtgtcgat gcccagcagc gactgcatgc cctcgtccca
     1381 cagcgcgggg atgtcgtcgg ccgcgatgct gccggcgatc agctgcttct cgatcccata
     1441 gcgcaggatc acatgcgccg ggtaggtcag ctcgtcggcg tcgacgcgga tgaagcccgg
     1501 cgccacgcgc gtcatcagcc ggtgcaggtt gtcgggctcg aacgccggct ggtcgccgaa
     1561 gtgcttgcgc agcagcggcg ccagcagcgc ggcgaaggcg cggctgcggc cgagctgcat
     1621 ctcgaaggcc aggctctgac tctcatggat gcccatcgag cgcgcgtgcg ccaccggctg
     1681 gccgagccag acgcgcggca ggttctgctc gtagcgcgcg tggccggtct cgtggatcgt
     1741 gcccatcagg ctttgcagca gatcgtcttc gtggtagcgc gtcgtcaggc gcgtgtcttc
     1801 gggcacgccg ccgctgaacg ggtgcgcgct ctcgtccagg cgcccgccct cgaagtcgaa
     1861 cccgagcagc gtcatcacgt ccaggctcag cgcgcgctgc gtcgccttcg ggaacgggcc
     1921 ggtcgcctcg atcaccgttt cggtcgcctg gcgtccgcgc acctgcgcga ccagccccgg
     1981 caaccactgc tgcaggtcgc cgaacacccg gtcgacctcg gcgctggtgg tgcccggctc
     2041 gtactggtcc atcagcgcgt cgtaaggggc gaggccggtc tcgtcggcca ggcgctgcgc
     2101 gacctcgcgg ccgagcgcca gcacctcgcg gaagttggcc aggaagccgg cccagtcgtt
     2161 ggccgggcgc tgcgtgcgcc acgcgtgctc gcagcgcgcg ccggccagcg acagcttctc
     2221 gaccagcgag cgcggcagcg cgttcgcgct gagccactcg cgccggatct cgccgaggtt
     2281 ggcgcgcgcg aacgggtcga tgtcttcgcc ggcggcgcgc atgagcagca cggccaggtt
     2341 cgggtcggtg cgcatgcggt gcagcagccc ctccatctcg gccatcgcgt tggcgcgtgc
     2401 ggtgttgccc ttgctcggca tcatcgcggc ctggtcccag ctggcgatcg actgcaggtg
     2461 ctggaagtgg tgcagccggg tccagatgcc ggtgagttcc tcgtacgcgg tgtggttcat
     2521 cgcctgttgc ctcgccatgg cctcgaacgg ccgttgtcga tcagtgcatg
//
LOCUS       DATMJB010000342         2406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_66755, whole genome shotgun sequence.
ACCESSION   DATMJB010000342 DATMJB010000000
VERSION     DATMJB010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66755"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /gene="mutL"
                     /locus_tag="VNU48_07445"
     CDS             <1..1179
                     /gene="mutL"
                     /locus_tag="VNU48_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559559.1"
                     /note="This protein is involved in the repair of
                     mismatches in DNA. It is required for dam-dependent
                     methyl-directed DNA mismatch repair. Promotes the
                     formation of a stable complex between two or more
                     DNA-binding proteins in an ATP-dependent manner without
                     itself being part of a final effector complex; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HWI11147.1"
                     /translation="QRWLDVLGEEFVEHSRAIALDLGVLRLSGRTGTPEAARARADQQ
                     YVYVNGRFVRDKLIAHGVRSAYEDVLHGGRQPAYLLFIEIAPDRVDVNVHPTKVEVRF
                     RDSREVHQAVRRAVETALAIPKAGLAAGEVGIETPPAVPVPQRYDAAQQASLGLQPGA
                     TRYAAHEPMPRWNTLPAALPAAFDAALPAAPVAADAWPLGKAIAQIAGIFVLAENAQG
                     LVIVDMHAAHERIVYERLKASLAARGGAAIDAQPLLIPATFAATADEIATAQAQADNL
                     HALGLDVSLLSASVLAVRSHPAALAGGDVVELARSVLAELAQFGASSVIQRAQHEILS
                     TMACHGAVRANRQLTLDEMNALLRDMERTDRADQCNHGRPTWRQITLKELDGLFWRGR
                     "
     gene            1330..>2406
                     /locus_tag="VNU48_07450"
     CDS             1330..>2406
                     /locus_tag="VNU48_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HWI11148.1"
                     /translation="MSTDAKCPFNHAAGGGTTNKDWWPGQLQLGLLSQHSDKSDPLGT
                     RFDYADEFKKLDYFALKKDLLALMTDSQDWWPADFGHYGPQFIRMAWHAAGTYRTSDG
                     RGGGGRGQQRFAPLNSWPDNVNIDKSRRLLWPIKQKYGQKISWADLMLLTGNVALESM
                     GFRTFGFGAGREDVWEPDNDVNWGNETTWLATDKRFSGDRDLDQPLGATHMGLIYVNP
                     EGPNASGDPVAAAKDIRATFGRMAMNDEETVALIAGGHSFGKTHGAAPESHKGPEPEG
                     APLEAQGLGWNSSFGSGHGKDTISSGLEVTWTTTPARWSNDFFEHLFRYEWELTQSPA
                     GAKQWVAKDAPEIIPDAHIPGKKHK"
BASE COUNT          403 a          864 c          831 g          308 t
ORIGIN      
        1 cagcgctggc tcgacgtgct cggcgaggag ttcgtcgagc acagccgggc gatcgcgctc
       61 gacctgggcg tgctgcgcct cagcggccgc accggcacgc ccgaggcggc acgcgcgcgc
      121 gccgaccagc agtacgtgta cgtcaacggc cgcttcgtgc gcgacaagct gatcgcgcac
      181 ggcgtgcgct cggcctacga agacgtgctg cacggcggcc gccagccggc gtacctgctg
      241 ttcatcgaga tcgcgcccga ccgggtcgac gtgaacgtgc acccgaccaa ggtcgaggtg
      301 cgcttccgcg actcgcgcga ggtgcaccag gcggtgcggc gcgcggtcga gacggcgctg
      361 gcgattccga aggccgggct cgccgcgggc gaggtgggta tcgagacgcc gccggcggtg
      421 cctgtgccgc agcgctacga cgccgcgcag caggcctcgc tcggcctgca gcccggtgcg
      481 acgcgctacg cggcgcacga gccgatgccg cgctggaaca ccctgcccgc ggcgctgccg
      541 gccgcgttcg acgccgccct gccggccgcg ccggtcgccg ccgacgcctg gccgctcggc
      601 aaggcgatcg cgcagatcgc cggcatcttc gtgctcgccg agaacgcgca gggcctggtg
      661 atcgtcgaca tgcacgccgc gcacgagcgc atcgtctacg agcgcctgaa ggcgagcctc
      721 gcggcgcgcg gcggcgcggc gatcgacgcg cagccgctgc tgatcccggc gaccttcgcg
      781 gccaccgccg acgagatcgc gaccgcccag gcgcaggccg acaacctgca cgcgctgggc
      841 ctcgacgtgt cgctgctctc ggcgtcggtg ctggcggtgc gctcgcatcc ggccgcgctg
      901 gccggcggcg acgtcgtcga gctcgcgcgc agcgtgctcg ccgagctcgc gcagttcggc
      961 gccagcagcg tgatccagcg tgcgcagcac gagatcctgt cgacgatggc ctgccacggc
     1021 gcggtgcgcg cgaaccggca gctgacgctc gacgagatga acgcgctgct gcgcgacatg
     1081 gagcgcaccg accgcgccga ccagtgcaac cacggccgac cgacctggcg ccagatcaca
     1141 ctgaaggaac tcgacggcct gttctggcgc gggcgctgaa cgcccggtgt tgccgaggtc
     1201 gatgtccggc atcacgaagt tagctgttct caattggaag atattcgacc atcgtcgtag
     1261 gcctgagaac aggtcaacgg cgcggtggcc cgcgcgacgt tcagcaccca acaaccgaag
     1321 gacagagcca tgtcaaccga cgccaagtgc ccgttcaacc acgccgccgg tggcggcacg
     1381 acgaacaagg actggtggcc cggccagctt cagctggggt tgctcagtca gcactcggac
     1441 aagtccgacc cgctgggcac gcgcttcgac tacgccgatg aattcaagaa gctcgactac
     1501 ttcgcgctca agaaggatct gctggcgctg atgaccgact cgcaggactg gtggccggcc
     1561 gacttcggcc actacggccc gcagttcatc cgcatggcct ggcacgccgc cggcacctac
     1621 cgcacgtctg acggccgggg cggcggcggc cgtgggcagc agcgcttcgc gccgctcaac
     1681 agctggcccg acaacgtgaa catcgacaag tcgcggcgcc tgctctggcc catcaagcag
     1741 aagtacggcc agaagatcag ctgggccgac ctgatgctcc tcaccggcaa cgtggcgctg
     1801 gagtcgatgg gcttccgcac cttcggcttc ggcgccggcc gcgaggacgt gtgggagccc
     1861 gacaacgacg tcaactgggg caacgagacc acctggctgg ccaccgacaa gcgcttcagc
     1921 ggtgatcgcg atctcgacca gccgctgggc gccacgcaca tgggcctgat ctacgtcaac
     1981 ccggaaggcc cgaacgcgag cggcgacccg gtggccgccg ccaaggacat ccgcgctacc
     2041 ttcggccgca tggcgatgaa cgacgaggag accgtcgccc tgatcgccgg cggccacagc
     2101 ttcggcaaga cgcacggcgc cgcgccggaa tcgcacaagg gccccgagcc cgaaggcgcg
     2161 ccgctcgagg cgcagggcct gggctggaac agcagcttcg gcagcggcca cggcaaggac
     2221 accatttcca gcggcctgga ggtcacctgg accaccacgc cggcgcgctg gagcaacgac
     2281 ttcttcgagc acctgttcag gtacgagtgg gaactgaccc agtctcccgc cggggcgaag
     2341 cagtgggtgg ccaaggacgc gccggagatc attcccgacg cgcacattcc cggcaagaag
     2401 cacaag
//
LOCUS       DATMJB010000343         5199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_60773, whole genome shotgun sequence.
ACCESSION   DATMJB010000343 DATMJB010000000
VERSION     DATMJB010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60773"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VNU48_07455"
     CDS             <1..225
                     /locus_tag="VNU48_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWI11149.1"
                     /translation="HGGVMDCLYRAAARVALDAPRSWQLGNAAINRLLYTPQGFTLIG
                     WSDDYHLDDDDAQGEYADVDAPPKPARAAV"
     gene            222..1343
                     /locus_tag="VNU48_07460"
     CDS             222..1343
                     /locus_tag="VNU48_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HWI11150.1"
                     /translation="MNTRPAARVGDPVDAIDTPALVIDLDAMERNLNAMAVFARERGL
                     LLRPHAKMHKSAAIATLQIAAGAVGVCVQKVDEAQALADAGIADLYISNEVVASAKLA
                     RLAALAGRVKLAVAVDSILGIERLATALKAAGSSADVFVEIDVGQGRCGVAPAAAGAL
                     AHQVVAHGLRFAGLQAYHGRAQHLRGAEARAAAIRHAAMLVRAAQASITSAGMRCPLV
                     TGAGSGSFAFEAASGVYDELQCGSYLFMDRDYADNEATPNAPAFEHALFVKSQVMSRG
                     ASHAVVDAGHKAHAVDSGLPRVWQRELVFANGGDEHGILTPRASGGALPELGETVWLV
                     PGHCDPTVNLHDHYIALRGGLAQGTVEAVWTIDARGRLR"
     gene            1368..2465
                     /locus_tag="VNU48_07465"
     CDS             1368..2465
                     /locus_tag="VNU48_07465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWI11151.1"
                     /translation="MTGPQIIVLATPLFFLLIGIEYLVGRRRGRDTYRLNDALNSIGL
                     GVLSQLAGVFSAALTLGVYALVFQHASLWQLPADAPWVWLVALLFYDFCYYWHHRFGH
                     TVALLWAAHVVHHQSEDYNLSTALRQTSSGWLAGWLFYLPLALLGFPPLVFGAVALID
                     LLYQYWVHTQQIGRLGGFDRWFCAPSNHRVHHAVNDRYLDKNYGGILIVWDRLFGSYA
                     EEDDAEPCVYGTRGPLRSWNPVWANLHLYAEMARDTWRASSGADRLRIWFEHPGWRPA
                     DVAAKWPKPAFDIRRVERFDPPMTRAAQLAAVLAFAALLGATTAFLWHAHRLSLTQQL
                     AVAALVVALLWVVGWLSERRAHDVNVSVFSR"
     gene            2696..3496
                     /locus_tag="VNU48_07470"
     CDS             2696..3496
                     /locus_tag="VNU48_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWI11152.1"
                     /translation="MTRALNLLERLAQRREPMSLAQLTSELALPKSSVHGLCNTLMSF
                     GYLRRQSDGAFLIGPRVMGLAEAFVSGTDVAQEFNALWADAGAAPEETVVLSVLSGTD
                     ALYVAVRNSARPLGLAFNVGMRLPAYLSGSGKANLAQRDPEEVRRLFADGLHTHLTRK
                     GPREVDTLLKELALTRKRGYSIDDESVREGVYAFGAPVFDATGQAVAAVAVCINKALL
                     GADRGGRHRDAVLAVARTLSQRLGGEVRTGPATAVVRRRKVGGIHERW"
     gene            3483..4259
                     /locus_tag="VNU48_07475"
     CDS             3483..4259
                     /locus_tag="VNU48_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI11153.1"
                     /translation="MSGGDFILETRALTKEFKGFVAVSKVDLKVRRGSIHALIGPNGA
                     GKTTCFNLLTKFLTPTSGEILFDGADITAEPPAVIARRGVIRSFQISAVFPHLSVLEN
                     VRVALQRTLGTSFHFWKPERSLRTLDARALELLREVDLESYAGIPAVELSYGRKRALE
                     IATTLAMDPKLMLLDEPTQGMGLEDVDRIRQLIKKVAASRTVLMVEHNMSVVASIADT
                     ITVLQRGATLAEGPYAEVSKNPAVIEAYMGSAKTELKGAH"
     gene            4341..5045
                     /locus_tag="VNU48_07480"
     CDS             4341..5045
                     /locus_tag="VNU48_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI11154.1"
                     /translation="MATPFLEVKDLQGWYGESHVLHGVNFHVDEGEVVTLLGRNGAGR
                     TSTMRAIMGLIGRRSGSIKVRGTETVGLATHKIARLGLGYCPEERGIFSSLSCEENLM
                     LPPQLAEGGMSVEQIYAMFPNLKERAGSQGTRLSGGEQQMLAVARILRTGAKLLLLDE
                     ISEGLAPVIVHKLAEMVVALRKQGYTIVMVEQNFVFAAPLADRFMVMEHGQVIQAFTQ
                     AELPARMERLHEYLGV"
BASE COUNT          764 a         1863 c         1819 g          753 t
ORIGIN      
        1 cacggcggcg tgatggactg cctgtaccgc gccgccgcgc gcgtcgcgct cgacgcgccg
       61 cgctcgtggc agctcggcaa cgccgcgatc aaccggctgc tctacacgcc gcagggcttc
      121 acgctgatcg gctggagcga cgactaccac ctcgacgacg acgacgcgca gggcgagtac
      181 gccgatgtcg atgcgccgcc gaagccggcg cgggcggccg tgtgaacacc aggcccgctg
      241 cgcgggtcgg cgacccggtc gatgcgatcg acacgccggc gctggtgatc gatctcgacg
      301 cgatggaacg caacctgaac gcgatggccg tgttcgcacg cgagcgcggc ctgcttctca
      361 ggccgcacgc gaagatgcac aagagtgccg cgatcgcgac gctgcagatc gcggccggcg
      421 cggtcggcgt gtgcgtgcaa aaggtcgacg aggcgcaggc cctggccgac gccggcatcg
      481 ccgatctcta catcagcaac gaggtcgtcg cgagcgcgaa gctcgcgcgg ctggccgcgc
      541 tggccgggcg cgtcaagctc gcggtggcgg tcgattcgat cctgggcatc gaacgcctgg
      601 ccacggcgct gaaggcggcc ggcagcagcg ccgacgtgtt cgtcgagatc gatgtcggcc
      661 agggtcgctg cggcgtcgcg ccggcggcgg ccggggcgct cgcgcaccag gtcgtcgcgc
      721 acggcctgcg cttcgccggg ctgcaggctt accatggccg cgcgcagcac ctgcgcggcg
      781 ccgaggcgcg cgctgcggcg atccgccacg ccgcgatgct ggtgcgcgcc gcgcaggcga
      841 gcatcacctc ggcggggatg cgctgcccgc tcgtcaccgg cgccggcagc ggcagcttcg
      901 cgttcgaggc cgcgagcggc gtctacgacg agctgcagtg cggcagctac ctgttcatgg
      961 accgcgacta cgccgacaac gaggcgacgc cgaacgcgcc ggccttcgag cacgccctct
     1021 tcgtcaagtc gcaggtgatg agccgcggcg cgtcgcacgc ggtggtcgac gccggccaca
     1081 aggcgcacgc ggtcgactcg ggcctgccgc gcgtctggca gcgcgagctg gtgttcgcca
     1141 acggtggcga cgagcacggc atcctgacgc cgcgcgcgtc gggcggcgcg ctgcccgagc
     1201 tcggcgagac ggtctggctc gtgccgggcc actgcgaccc gaccgtgaac ctgcacgatc
     1261 actacatcgc gctgcgcggc gggctggcgc agggcacggt cgaggcggtc tggaccatcg
     1321 acgcgcgcgg tcgcctgcgc tgaacccgac gcggcagaat cgcggccatg accggcccgc
     1381 agatcatcgt tctcgccacg ccgctgttct tcctgctgat cggcatcgaa tacctcgtcg
     1441 gccgccgccg cgggcgcgac acctaccggc tcaacgatgc gctgaacagc atcgggctgg
     1501 gcgtgctgag ccagctcgcc ggcgtgttca gcgcggcgct gacgttgggt gtctacgcgc
     1561 tggtgttcca gcacgcgtcg ctctggcagc tgccggcgga cgcgccgtgg gtctggctcg
     1621 tcgcgctgct tttctacgac ttctgctact actggcacca ccgcttcggc cacaccgtcg
     1681 cgctgctgtg ggccgcgcac gtggtgcacc accagagcga ggactacaac ctgtcgaccg
     1741 cgctgcgtca gaccagcagc ggctggctcg ccggttggct gttctacctg ccgctggcgc
     1801 tgctcggttt tccgccgctg gtgttcggcg cggtcgcgct gatcgacctg ctgtaccagt
     1861 attgggtcca cacccagcag atcggccggc tcggcgggtt cgaccgctgg ttctgcgcgc
     1921 cgagcaacca ccgcgtgcat cacgccgtca acgaccgcta cctcgacaag aactacggcg
     1981 gcatcctgat cgtctgggat cgcctgttcg gcagctacgc cgaagaggac gacgccgaac
     2041 cctgcgtcta cggcacgcgc gggccgctgc gcagctggaa cccggtctgg gcgaacctgc
     2101 acctctacgc cgagatggcg cgcgacacct ggcgcgcgtc aagcggggcc gatcggctgc
     2161 gcatctggtt cgagcacccc ggctggcgcc ccgccgacgt cgccgcgaag tggccgaagc
     2221 cggccttcga catccgccgc gtcgagcgct tcgacccgcc gatgacgcgc gccgcgcagc
     2281 tcgccgcggt gctggcgttc gctgcgctgc tgggcgcgac caccgcgttc ctgtggcatg
     2341 cgcatcgcct gagcctgacg cagcaactcg cggtggccgc gctcgtggtc gcgctgctgt
     2401 gggtggtcgg ctggctcagc gagcgccgcg cgcacgatgt aaacgtctcg gtgttttccc
     2461 ggtaaaaacg cgcgcggggc ttgcgggcgc cgcgggactg aatagactgc ctcccagccc
     2521 catcgaaccg cgttcaacct gttgaacttt cgagatggcc ccgcgaagga ggtccgcgct
     2581 gctgctgtgc cgcggtcgac cgcccccgga gacgatcctg cacaccacgc ccgcatcgcc
     2641 gcgcagcccc gcccgtcgcc cgccgcagcc cgcggccgcg ccgctggtgc cggccgtgac
     2701 gcgggcgctg aacctgctcg agcgcctggc gcagcggcgc gagccgatga gcctggcgca
     2761 gctgacgagc gagctcgcgc tgcccaagag cagcgtgcac ggcctgtgca acacgctgat
     2821 gtcgttcggc tatctgcgcc gccagagcga cggcgcgttc ctgatcggcc cgcgcgtgat
     2881 gggtctggcc gaggccttcg tctccggcac cgacgtggcg caggagttca acgcgctgtg
     2941 ggccgacgcc ggcgccgcgc ccgaagagac cgtcgtgctg tcggtgctca gcggcaccga
     3001 cgcgctgtac gtcgcggtgc gcaacagcgc gcgcccgctc ggcctggcgt tcaacgtcgg
     3061 catgcggctg ccggcctatc tgtcgggcag cggcaaggcg aacctggcgc agcgcgaccc
     3121 cgaggaagtg cgccgcctgt tcgccgacgg cctgcacacc cacctgacgc gcaagggccc
     3181 gcgcgaggtc gacacgctgc tgaaggaact cgcgctcacg cgcaagcgcg gctacagcat
     3241 cgacgacgaa agcgtgcgcg agggcgtcta tgcgttcggg gcgccggtgt tcgacgccac
     3301 cggccaggcc gtggccgcgg tcgcggtctg catcaacaag gccctgctcg gcgccgaccg
     3361 cggcgggcgc caccgcgacg cggtgctcgc ggtcgcgcgc acgctgtcgc agcgcctggg
     3421 tggcgaggtc aggacggggc ccgcgacggc cgtggtgcgg cgacgcaagg tcgggggcat
     3481 tcatgagcgg tggtgatttc atcctcgaga cccgcgcgct gaccaaggag ttcaagggct
     3541 tcgtcgctgt cagcaaggtc gacctgaagg tgcggcgcgg cagcatccat gcgctgatcg
     3601 ggccgaacgg cgccggcaag acgacctgct tcaacctgct gaccaagttc ctgacgccca
     3661 ccagcggcga gatcctgttc gacggcgccg acatcaccgc cgagccgccg gccgtgattg
     3721 cacgccgcgg cgtgatccgc tcgttccaga tctccgcggt gttcccgcac ctgagcgtgc
     3781 tcgagaacgt gcgcgtcgcg ctgcagcgca cgctcggcac ctcgttccac ttctggaagc
     3841 ccgagcgcag cctgcgcacg ctcgacgcgc gggccctcga gctgctgcgc gaggtcgacc
     3901 tcgagagcta cgccggcatc cccgccgtcg agctcagcta cgggcgcaag cgcgcgctcg
     3961 agatcgccac gacattggcg atggacccga agctgatgct gctcgacgag ccgacccagg
     4021 gcatgggcct ggaggacgtc gaccgcatcc gccagctgat caagaaggtc gccgccagcc
     4081 gcaccgtgct gatggtcgaa cacaacatga gcgtggtggc cagcatcgcc gacacgatca
     4141 cggtgctgca gcgcggcgcc acgctcgccg aggggccgta tgccgaggtc tcgaagaacc
     4201 cggcagtgat cgaggcctac atgggcagcg cgaagaccga gctgaaggga gcgcattgat
     4261 gacgaccccc acgtcgcttc gctcctgccc cccgaggggg cgcaggcctc ccttgggaac
     4321 ggcccggcgg gagccctgag atggccaccc ccttcctcga agtcaaggac ctgcagggct
     4381 ggtacggcga atctcacgtg ctgcacggcg tgaacttcca tgtcgacgaa ggcgaggtcg
     4441 tgaccctgct cggccgcaac ggcgcgggcc gcacgagcac gatgcgcgcg atcatgggcc
     4501 tgatcgggag gcgttcgggc tcgatcaagg tgcgcggcac cgagaccgtc ggcctggcga
     4561 cgcacaagat cgcccgcctc ggcctgggct actgccccga ggagcgcggc atcttctcca
     4621 gcctgtcgtg cgaggagaac ctgatgctgc cgccgcaact cgccgagggc ggcatgagcg
     4681 tcgagcagat ctacgcgatg ttcccgaacc tgaaggagcg cgccggcagc cagggcacgc
     4741 gcctgtcggg cggcgagcag cagatgctcg cggtcgcgcg catcctgcgc accggtgcga
     4801 agctgttgct gctcgacgag atctccgagg gcctcgcgcc ggtgatcgtg cacaagctcg
     4861 ccgagatggt cgtggcgctg cgcaagcagg gctacacgat cgtgatggtc gagcagaact
     4921 tcgtcttcgc cgcaccgctg gccgaccgct tcatggtgat ggagcacggc caggtgatcc
     4981 aggcgttcac gcaggccgaa ctgccggccc gcatggaacg cctgcacgag tacctgggcg
     5041 tctgaaccac agcgccgcca ttcccgtttg cccatcccca cgaaggagat cccgatgacc
     5101 ctgaagaccc tttccgccgc ctgcgccgtc gccgcgacca cgctgttcgg cgcctccgcc
     5161 caggcccaga tctcgggcga cgtgatcaag atcggcttc
//
LOCUS       DATMJB010000344         2859 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_518433, whole genome shotgun sequence.
ACCESSION   DATMJB010000344 DATMJB010000000
VERSION     DATMJB010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518433"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..178
                     /locus_tag="VNU48_07485"
                     /pseudo
     CDS             38..178
                     /locus_tag="VNU48_07485"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
     gene            complement(191..811)
                     /locus_tag="VNU48_07490"
                     /pseudo
     CDS             complement(191..811)
                     /locus_tag="VNU48_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
     gene            complement(900..2180)
                     /locus_tag="VNU48_07495"
     CDS             complement(900..2180)
                     /locus_tag="VNU48_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HWI11155.1"
                     /translation="MLQQIIDRRLAGKNKSIGNRERFLRRHHNQVRDAVRRAVDNRGI
                     RDMERGEDIHIPKKDISEPVFGHGPGGTREMVHPGNQEYVAGDQIERPKGGGGKGGGK
                     GEASDSGEGEDDFVFHLTKEEFMQIFFDDLALPRMTKTQLAETPEWKSHRAGFVSDGT
                     PSNLSVVRSMRGAIGRRLAIGAGSRGELRALEEELERVLSEPVSALRDAQRDDLQLRI
                     SALKNRLARIPYLDPIDLRFRSRVRVPVPTTKAVMFCLMDVSGSMDEARKELSKRFFI
                     LLYLFLTRHYDKIDLVFIRHHTQAQEVSEEDFFHARETGGTVVSSALVLMDEIIKARY
                     SPTEWNIYGAQASDGDNWHHDSGRCRELLTENLLPVCRYFAYVQVAEEEQNLWEEYSR
                     LQQTHPAFAMRKVTQASQIYPVFRELFKKESVAA"
     gene            complement(2210..>2859)
                     /locus_tag="VNU48_07500"
     CDS             complement(2210..>2859)
                     /locus_tag="VNU48_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HWI11156.1"
                     /translation="AVFNYDQTEIAANPVHLMYVLEQRLAREDLPDEVRRRCVEHIKG
                     YLAPRYAEFIGKEIQTAYLESYSEYGQNIFDRYVTYADYWIQDHEYRDTDTGEVFDRA
                     SLNAELEKIEKPAGISNPKDFRNEIVNFVLRARANNAGNNPVWSSYEKLRTVIEKKMF
                     SNTEELLPVISFNAKASADEAKKHENFVNRMVDKGYTAKQVRLLCEWYLRVRKSS"
BASE COUNT          414 a          922 c          957 g          566 t
ORIGIN      
        1 gtgcatctcc tacctgatgg aggagaacac gacggcgatg caggcgctcg tcatcgccca
       61 tgccgcgtac ggccacaaca gcttcttcaa gggcaactac ctgttccgcc tctggaccga
      121 cgcgtcgtcg atcatcgact acctcgtcta cgcgcggaac tacatcgccg agtgcgagga
      181 gcgccacggc atggggccgg tcgaggacct gctcgacaga atcctcgccg tggcgctcct
      241 cgcactcgct gacgaactgc cgcgcgtaca ccaggtaatc gatgatggac gacgcgtcgg
      301 tccacatccg gaacaggtag ttgcccttga agaagctgtt gtgcccgtag gccgcgtggg
      361 cgatcaccag cgcctgcatc gccgtcgtgt tctcctccat caggtagctg atgcaggggt
      421 cggagttgat gacgatctcg taggccaggc ccatgtggcc gcgcttgtag ttcttctcgt
      481 tggcgatgaa ctccttgccg tagctccagt gacggtagtt caccggcatg ccgaccgacg
      541 cgtaggcgtc catcatctgc tcggcggtga tgatctcgag ctggttcggg taggtgtcga
      601 ggtcgaagcg cagcgcggcc gcgcggatcg cctcgtggta ctgctcgatc agctcgaagg
      661 tccagtcgct cggatcgggc agcggcgcgg ccgggcgcgg ccccggcggg cgcgggtgct
      721 ggagcggacc ggcctcggcc ttgcggcggt cgcgcgggta gggcgccggc gcggcgacgc
      781 tgacgcggcg ttctcctggg aaggtgctca tgacgagtgc tccttgattt gggcaccctg
      841 cgcgctgcgc gcgatcccgc cgagcgggag cgaacgcctt gggaacagcc cggcggcgtt
      901 catgcggcca cgctctcttt cttgaagagc tcgcggaata ccgggtagat ctgcgaggcc
      961 tgcgtgacct tgcgcatcgc gaaggcaggg tgcgtctgct gcaggcgcga atactcttcc
     1021 cacaggttct gctcctcctc ggcgacctgc acgtacgcga aatagcggca caccggcagc
     1081 aggttctcgg tcagcagctc gcggcagcgc ccgctgtcgt ggtgccagtt gtcgccgtcg
     1141 ctggcctgcg cgccgtagat gttccactcg gtcggcgagt agcgcgcctt gatgatctcg
     1201 tccatcagca ccagcgcgct cgacaccacg gtgccgccgg tctcgcgggc gtggaagaag
     1261 tcttcctcgc tgacttcctg cgcctgcgtg tggtggcgga tgaagacgag gtcgatcttg
     1321 tcgtagtgcc gggtcaggaa caggtagagc aggatgaaga agcgcttgga cagctccttg
     1381 cgcgcctcgt ccatcgagcc cgagacgtcc atcaggcaga acatcaccgc cttggtggtc
     1441 ggcaccggca cgcgcacgcg gctgcgaaag cgcaggtcga tcgggtcgag gtagggaatg
     1501 cgcgcgagcc ggttcttcag tgcgctgatg cgcagctgca gatcgtcgcg ttgcgcatcg
     1561 cgcagggcac tgaccggctc ggacagcacg cgctcgagct cttcctcgag cgcgcgcagt
     1621 tcgccgcgcg agccggcccc gatcgccagg cgccggccga tcgcgccgcg catcgaacgc
     1681 acgacgctga ggttgctcgg cgtgccgtcg ctgacgaagc cggcgcggtg gctcttccac
     1741 tcgggcgtct ccgccagttg cgttttcgtc atgcgcggca gcgcgaggtc gtcgaagaag
     1801 atctgcatga actcttcttt ggtgaggtgg aagacgaagt cgtcctcgcc ctcgccggag
     1861 tcgctggcct cgcccttgcc gccgcccttg ccgccgccgc ccttggggcg ctcgatctga
     1921 tcgccggcca cgtactcctg gttgccgggg tggaccatct cgcgcgtgcc gccggggccg
     1981 tggccgaaca ccggctcgga gatgtccttc ttcgggatgt gaatgtcctc gccgcgctcc
     2041 atgtcgcgga tgccgcggtt gtcgacggcg cgccgcaccg cgtcgcgcac ctggttgtgg
     2101 tggcggcgca ggaagcgctc gcggttgccg atcgacttgt tctttcccgc gaggcgccga
     2161 tcgatgatct gctgaagcat gtgttctccg gtggtcgcgc gcgccccgat cagctgctct
     2221 tgcgcaccct caggtaccac tcgcacagca ggcgcacctg cttcgcggtg tagcccttgt
     2281 cgaccatgcg attgacgaag ttctcgtgct tcttcgcctc gtcggcgctc gccttggcat
     2341 tgaagctgat gaccggcagc aactcttcgg tgttgctgaa catcttcttc tcgatcacgg
     2401 tgcgcagctt ctcgtagctc gaccacaccg ggttgttgcc ggcgttgttg gcgcgcgcgc
     2461 gcagcacgaa gttgacgatc tcgttgcgga agtccttcgg gttgctgatg cccgcgggct
     2521 tctcgatctt ctcgagctcg gcgttcagcg aggcgcggtc gaacacttcg ccggtgtcgg
     2581 tgtcgcggta ctcgtgatcc tggatccagt agtcggcgta ggtgacgtag cggtcgaaga
     2641 tgttctgccc gtactcgctg tagctctcga ggtacgcggt ctggatctcc ttgccgatga
     2701 actcggcata gcgcggtgcc agatagccct tgatgtgctc gacgcagcgc cggcgcacct
     2761 catcgggcag gtcttcacgc gcgaggcgct gctccagcac gtacatcaga tgcaccggat
     2821 tggcggcgat ctcggtctgg tcgtaattga acaccgcgg
//
LOCUS       DATMJB010000345         2961 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_778389, whole genome shotgun sequence.
ACCESSION   DATMJB010000345 DATMJB010000000
VERSION     DATMJB010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778389"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="VNU48_07505"
     CDS             complement(<1..773)
                     /locus_tag="VNU48_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI11157.1"
                     /translation="MTTTAYTARRASRSRFVTLRGLRHHVLEWGDAALATPQRPSLLM
                     QHGWMDVAASFQFVVDALAADRHVLAFDWRGFGLTDSAAADSYWLPDYLGDLDAAFDA
                     LFAGMWPIDLLGHSMGGNTAMLYAGLRPQRVRRLVNLEGFGMPTTKPSQSPKRVVQWL
                     DELKQPVELRDYASLAEVAARLRKTNPLLPPDRAAWVAQYWSRARPDGRFEILADAAH
                     KRISPYLYQRDEALETWKLIEAPVLWVEGDQTDTVRWWGT"
     gene            844..1353
                     /locus_tag="VNU48_07510"
     CDS             844..1353
                     /locus_tag="VNU48_07510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI11158.1"
                     /translation="MNPPPRFTLRPAELRDVGAIVQLIRDLAEFEQLTHLLQVTPEKL
                     RPQLFGERPAAEALVAETGDGKGGGEVVAFALFFTNFSTFLAQPGLYLEDLYVKPEHR
                     GSGIGRALLKRLAALAVERQHGRFEWSVLDWNEHAIRFYQGMGATVMPEWRICRVTGD
                     ALQAMGEVA"
     gene            1350..>2961
                     /locus_tag="VNU48_07515"
     CDS             1350..>2961
                     /locus_tag="VNU48_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI11159.1"
                     /translation="MTDRHAELHRAFRWLVPADFNIAHVCCTRWARDTPDALAIRWEH
                     EDGRSALYTYRELQREANRLSNALTRLGVARGDRVAIVMPQRFETAVAHIALYQLGAI
                     AMPLSMLFGPEALEYRLNDSAARVAIADESGIANLRAARAACPVLQTVLAVGDAQGQG
                     DLDWSAALARERRVFAPVRTSADDAAVLIYTSGTTGPPKGALIPQRALIGNLSGFVAS
                     QNWFGFRPPSLGKGERGKGGVSTGNQKSRSVFWSPADWAWTGGLMDALLPTLYFGRPI
                     VAYQGRFAPETAFALMQRHRVTHTFLFPTALKAMMKALPAPRERYRLDLRAIMSAGEA
                     VGDAVFGWCGQQLGVTVNEMFGQTEMNYIVGNSHAMWPARPGSMGKPYPGHRVAVIDD
                     DGRECPRGTPGDIALHRRDIHGVPDPVFFLGYWNNEAATRAKYVGAMDDSWCRTGDMA
                     VMDADGYLWYHGRSDDMFKAAGYRIGPSEIENCLVKHPAVANAAVVPKPDAQRGALVK
                     AYVVLAAGFDGTPALVDELQQHVRGKLAP"
BASE COUNT          427 a         1051 c         1077 g          406 t
ORIGIN      
        1 gtgccccacc agcgcacggt gtcggtctgg tcgccctcga cccacagcac cggggcctcg
       61 atcagcttcc aggtctccag cgcctcgtcg cgctgataga ggtacgggct gatgcgcttg
      121 tgcgccgcgt cggcgaggat ctcgaagcgg ccgtcgggac gcgcgcgcga ccagtactgt
      181 gccacccagg ccgcgcggtc cggcggcagc agcggattgg tcttgcgcag ccgtgccgcg
      241 acctcggcca ggctggcgta gtcgcgcagc tcgacgggct gcttcagctc gtcgagccac
      301 tgcacgacgc gcttgggcga ctgcgacggc ttcgtcgtcg gcatgccgaa gccctcgagg
      361 ttgacgagcc ggcgcacgcg ctgggggcgc agcccggcgt acagcatcgc cgtgttgccg
      421 cccatgctgt ggccgagcag atcgatcggc cacatgccgg cgaacagcgc atcgaacgcg
      481 gcgtcgaggt cgcccaggta gtccggcagc cagtaggagt cggcggccgc cgagtcggtc
      541 aggccgaagc cgcgccagtc gaaggcgagc acgtggcggt cggcggcgag cgcgtcgacg
      601 acgaactgga acgacgcggc cacgtccatc cagccgtgct gcatcagcag cgacgggcgc
      661 tgcggcgtgg ccagcgcggc gtcgccccat tcgagcacgt ggtggcgcag gccgcgcagg
      721 gtgacgaagc ggctgcggct ggcgcggcgc gcggtgtagg cggtggtggt catcaccgca
      781 ctgtacgcgg cgcggcgcgg cggtgctgtc cgcgccgcga cagccgatgc gagaatgccc
      841 ccgatgaacc cgcccccccg cttcacgctg cgcccggccg agctgcgcga tgtcggcgcg
      901 atcgtccagc tgatccgcga cctggccgag ttcgagcagc tcacgcacct gctgcaggtc
      961 acgcccgaga agctgcgccc gcagctgttc ggcgagcggc cggcggccga ggcgctggtg
     1021 gccgagacgg gtgacggcaa gggcggcggc gaggtggtcg cgttcgcgct gttcttcacc
     1081 aatttctcga ccttcctggc gcagccgggc ctgtacctcg aagacctgta cgtcaagccc
     1141 gagcaccgcg gcagcggcat cggccgcgcg ctgctgaagc gcctggccgc gctcgccgtg
     1201 gagcgccagc acggccgctt cgaatggagc gtgctcgact ggaacgagca cgcgatccgc
     1261 ttctaccagg gcatgggcgc gaccgtgatg cccgaatggc gcatctgccg cgtcaccggc
     1321 gacgcactgc aggcgatggg cgaggtggcg tgacggatcg gcacgccgaa ctgcaccggg
     1381 cgttccgctg gctcgtgccg gcggacttca acatcgccca cgtctgctgc acgcgctggg
     1441 cccgcgacac gcccgacgcg ctcgcgatcc gctgggagca cgaggacggc cgcagcgcgc
     1501 tgtacaccta ccgcgagctg cagcgcgagg cgaaccgcct ttcgaatgca ctgacccggc
     1561 tcggcgtcgc gcgcggcgac cgcgtcgcga tcgtgatgcc gcagcgcttc gagaccgcgg
     1621 tcgcgcacat cgcgctgtac cagctcggcg cgatcgcgat gccgctgtcg atgctgttcg
     1681 ggcccgaggc gctggagtac cgcctgaacg acagcgccgc gcgcgtcgcg atcgccgacg
     1741 agagcggcat cgcgaacctg cgcgcggcgc gcgccgcctg cccggtgctg cagacggtgc
     1801 tcgcggtcgg cgacgcgcag gggcagggcg acctcgactg gagcgcggcg ctggcgcgcg
     1861 agcgccgcgt gttcgcgccg gtgcgcacga gcgccgacga cgccgcggtg ctgatctaca
     1921 ccagcggcac caccggcccg ccgaagggcg cgctgatccc gcagcgcgcg ctgatcggca
     1981 acctgagcgg cttcgtcgcg agccagaact ggttcggttt caggccccct tccctgggga
     2041 agggggagcg ggggaagggg ggtgtgtcta ctgggaacca gaagtcgcgt agcgtcttct
     2101 ggagccccgc cgactgggca tggacgggtg gcctgatgga tgcgctcctg cccacgctct
     2161 atttcggccg acccatcgtc gcctaccagg gccgcttcgc ccccgagacc gcgttcgcgc
     2221 tgatgcagcg ccaccgcgtc acccacacct tcctgttccc caccgcgctg aaggcgatga
     2281 tgaaggcgct gcccgcgccg cgcgagcgct accggctcga cctgcgcgcg atcatgagcg
     2341 ccggcgaggc cgtgggcgac gcggtgttcg gctggtgcgg gcagcagctg ggcgtgaccg
     2401 tcaacgaaat gttcggccag accgagatga actacatcgt cggcaactcg cacgcgatgt
     2461 ggccggcgcg gcccggcagc atgggcaagc cctatccggg ccatcgcgtc gcggtgatcg
     2521 atgacgacgg ccgcgagtgc ccgcgcggca cgcccggcga catcgcgctg caccggcgcg
     2581 acatccacgg cgtgcccgac ccggtgttct tcctcggcta ctggaacaac gaggccgcga
     2641 cgcgcgccaa gtacgtgggg gcgatggacg acagctggtg ccgcaccggc gacatggcgg
     2701 tgatggatgc cgacggctac ctctggtacc acggccgcag cgacgacatg ttcaaggccg
     2761 ccggttaccg catcggcccg agcgagatcg agaactgcct cgtcaagcac ccggcggtcg
     2821 cgaacgccgc ggtcgtgccc aagcccgatg cgcagcgcgg cgcgctggtc aaggcctatg
     2881 tcgtgctggc cgccggcttc gacggcacgc cggcgctcgt cgacgaattg cagcagcacg
     2941 tgcgcggcaa gctcgcgccc t
//
LOCUS       DATMJB010000346         3016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_362680, whole genome shotgun sequence.
ACCESSION   DATMJB010000346 DATMJB010000000
VERSION     DATMJB010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362680"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VNU48_07520"
     CDS             complement(<1..686)
                     /locus_tag="VNU48_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWI11160.1"
                     /translation="MTLPTTRRFEWPRARAAKAAPFIALLLAGGAAAAAPLCDKSEIR
                     LVNSMREIAQPYHANLDKGGRLFAKWAGIGPRYVLQLNHGDSDKQVSLMRGVLGAHPR
                     CTIFNVEPNADLVIKPMVKVASRSGAWIVTHWAHSEGLHPFDGNDQWIAHVAVDSLDA
                     GVAISRRLAEAMQGKGGIVALQGRLDTDPARQRFAGLQQVLKDMPDIKLLDQQTANWD
                     RTAAFPIVQT"
     gene            complement(694..>3016)
                     /locus_tag="VNU48_07525"
     CDS             complement(694..>3016)
                     /locus_tag="VNU48_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI11161.1"
                     /translation="RLVESNIIGVCFWDVTGEITEANDAFLDLVGYSQQDLHAGMVRW
                     AAMTPAEYRAADARAASELRQTGTFSLYEKEFIRQDGRRVPILMGGAFLQGSQQNGVA
                     FVLDLTERKRAETEREARQVAEAANQAKSEFLANMSHEIRTPMNAILGMSHLALQSGL
                     NPQQHNYVQKVHRSAESLLGIINDILDFSKIEAGHLDIERIPFELGDVMDSLANVMGM
                     KAEEKGLELVFALPPDLPNALVGDPSRLGQVLINLGNNAVKFTERGEVIVGVEMLARD
                     AGSVRLRFEVRDTGIGITAEEQQRLFKPFSQADSSTSRRFGGTGLGLAISHHLVRMMD
                     GELGVDSTAGHGSRFHFSANFGIGLETSAASPEGGLQGTRVLVVDDNDAAREVLVRMA
                     DSLGLRASAANGGHEAIQAVVAADACDTPFDLLLLDWKMPGMSGVDCAKQLARMPLAH
                     RPPTVLMLTAFSRDDMARRLAAEQLTVAATLTKPVTPSTLLDASLQAIGRPRQHAPRG
                     ELREEELQSNRAALAGARVLLVEDNTFNQELACDLLGRARIVVRIANNGREAIDMLAR
                     ERFDAVLMDCQMPVMDGYEATRELRSHPQWRDLPIIAMTANAMVGDREKVLAAGMNDH
                     VAKPINVAEMFATLARWVRPQVAAASSAFPGIESAAALAGVMGDEQLYRRLLGMFLDR
                     EASFAVRFGAARAAHDMPMARRLAHDLKSTSAILGATAVREAAEVLEQACADGARSAD
                     IDALLAVVVEHLDPVIAGLRALDAGAVQADAVH"
BASE COUNT          443 a         1037 c         1021 g          515 t
ORIGIN      
        1 caggtctgca cgatcgggaa ggcggcggtg cggtcccagt tggcggtctg ctggtcgagc
       61 agcttgatgt cgggcatgtc cttcagcacc tgctgcaacc cggcaaagcg ctggcgggcc
      121 gggtcggtgt ccagccggcc ctgaagcgcg acgatgccgc ccttgccctg catggcctcg
      181 gccagccgcc tggagatcgc cacgccggca tccaggctgt cgacggcgac gtgcgcgatc
      241 cactggtcgt tgccgtcgaa cggatgcagg ccttcgctgt gcgcccagtg cgtgacgatc
      301 caggcgccgg aacgactcgc caccttgacc atcggcttga tgaccaggtc ggcattcggc
      361 tcgacgttga agatcgtgca tctcggatgg gcgcccagca ccccccgcat cagcgagacc
      421 tgcttgtccg aatcgccatg gttgagttgc agcacgtagc gcggtccgat cccggcccat
      481 ttggcgaaca gccggccgcc cttgtcgagg ttggcgtgat agggctgcgc aatttcgcgc
      541 atcgaattga cgagccggat ctccgacttg tcgcacagcg gtgccgccgc ggcggccccc
      601 ccggccagca gcagcgcgat gaagggtgcg gccttggccg cacgggcacg gggccactcg
      661 aagcggcgcg tcgtcggcag ggtcatggtt gggtcaatgg accgcatcgg cctgcaccgc
      721 ccccgcgtcg agcgcgcgca ggccggcgat cacggggtcg agatgctcga ccaccaccgc
      781 caacagcgcg tcgatgtcgg ccgagcgggc gccgtcggcg caggcttgtt ccagcacttc
      841 ggcggcctct cggacggcgg tcgctccgag gatggcggag gtgctcttca ggtcgtgcgc
      901 cagccgccgg gccatgggca tgtcgtgcgc ggcacgggca gcgccgaaac ggaccgcgaa
      961 gctggcttcg cgatcaagga acatgcccag caagcgccga tagagctgct cgtcgcccat
     1021 caccccggcc agcgcagccg cgctctcgat gcccgggaaa gcgctgctcg ccgccgccac
     1081 ctgaggtcgg acccagcgcg ccagcgtcgc gaacatttcg gccacgttga tcggcttcgc
     1141 cacgtggtcg ttcatgccgg ccgccagcac tttttcgcgg tcgccgacca tcgcgttggc
     1201 cgtcatcgca atgatcggca ggtcgcgcca ctgcgggtgg ctgcgcaact cgcgcgtggc
     1261 ctcgtagccg tccatgaccg gcatctggca atccatcagc actgcgtcga agcgttcgcg
     1321 cgccagcatg tcgatggcct cgcgcccgtt gttggcgatc cggaccacga tccgcgcacg
     1381 gcccagcagg tcacacgcca gttcctggtt gaacgtgttg tcctcgacca gcagaacccg
     1441 cgcgccggcc agggcggcgc ggttgctctg caactcttct tcgcgcagtt cgccacgcgg
     1501 cgcatgctgc cgcggccggc cgatcgcctg caggctggca tcgagcagtg tcgagggcgt
     1561 gaccggcttg gtcagcgtcg ccgccacggt gagctgttcg gccgccagcc tgcgcgccat
     1621 gtcgtcgcgc gagaaggcgg tgagcatcag cacggtgggt ggccggtgcg ccagcggcat
     1681 gcgggccagc tgcttcgcac agtcgacgcc gctcatgccc ggcatcttcc agtcgagcaa
     1741 gagcaggtcg aagggcgtgt cgcacgcatc cgccgccacg acggcctgga tggcttcatg
     1801 gccgccgttg gcagccgacg cgcgcagccc gagcgagtcg gccatgcgca ccagcacctc
     1861 gcgcgcggcg tcgttgtcgt cgacgacgag cacgcgggta ccctgcaggc cgccctcggg
     1921 tgaagccgcg ctggtttcga gcccgatgcc gaagttcgcg ctgaagtgga accggctgcc
     1981 gtgcccggcg gtgctgtcca cgccgagttc gccgtccatc atgcgcacca ggtggtgact
     2041 gatcgccagg cccaggccgg tgccgccgaa gcggcggctc gtcgatgagt cggcctgcga
     2101 gaacggcttg aagagccgct gttgttcctc ggcggtgatg ccgatgccgg tgtcacgcac
     2161 ctcgaagcgc aggcgcaccg agcctgcgtc gcgcgcgagc atctcgacgc cgacgatgac
     2221 ctcgccccgt tcggtgaact tgaccgcgtt gttgcccagg ttgatcagca cctgcccgag
     2281 ccgcgagggg tcgcccacca gcgcgttggg caggtcgggc ggcaacgcga acaccagctc
     2341 caggcccttc tcttcggcct tcatgcccat cacattggcc aggctgtcca tgacatcgcc
     2401 gagctcgaac gggatgcgct cgatgtccag atggccggcc tcgatcttgg agaagtcgag
     2461 aatgtcgttg atgatgccca gcagcgattc ggccgatcgg tgcaccttct gcacgtagtt
     2521 gtgctgctgc gggttcagac cgctttgcag cgccaggtgc gacatcccca ggatcgcatt
     2581 catcggcgtg cggatctcgt ggctcatgtt ggccagaaat tcgctcttgg cctggttcgc
     2641 cgcttcggcc acctggcgtg cctcgcgttc ggtctcggcc cgcttgcgtt cggtcaggtc
     2701 cagcacgaat gcgacgccgt tctgctgtga accttgcagg aaggccccgc ccatcagaat
     2761 cggcacgcgc cggccgtcct ggcgaatgaa ttccttttcg tagagcgaga aggtgccggt
     2821 ctgacgcagt tccgaggccg cccgcgcatc ggccgcgcgg tattccgccg gcgtcatcgc
     2881 ggcccaccgg accatgccgg catgcaggtc ctgctggctg tagccgacca ggtcgaggaa
     2941 ggcgtcgttc gcttcggtga tctcgccggt gacgtcccag aagcacacgc cgatgatgtt
     3001 ggattcgacg agccgg
//
LOCUS       DATMJB010000347         3720 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_424881, whole genome shotgun sequence.
ACCESSION   DATMJB010000347 DATMJB010000000
VERSION     DATMJB010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424881"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1412)
                     /locus_tag="VNU48_07530"
     CDS             complement(198..1412)
                     /locus_tag="VNU48_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI11162.1"
                     /translation="MLLPRGADAGALPLLVARGLRAFADGYMALLLPTYLLAIGLGTL
                     EVGIVSTATMLGSALATLAVGAWGHRFASRRLLRGAALLMLATGLGFASLASFWPLLI
                     VAFVGTLNPSSGDVSVFLPLEHARLAACARGAARTALFARYSVVGALCASLGALAAAV
                     PDWLVRVAGVARLDALGGMFVVYGALGLTVFWLYRRLPSGETGRDDALLPTAALGPSH
                     AIVVRLAALFSVDAFAGGLVINALLSLWLLQRFGLSLTQAGVFFFWAGVLSAASQLAA
                     PLVAQRIGLLNTMVFTHIPANVCLVLAALAPSLPIALGLLFVRSALSQMDVPTRTAYV
                     MAVVTPAERAAAASFTAVPRSLAAALSPTLSGALFAAGWIALPLIGCGVLKIAYDLAL
                     WRAMRLNRIDGH"
     gene            1548..2537
                     /locus_tag="VNU48_07535"
     CDS             1548..2537
                     /locus_tag="VNU48_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HWI11163.1"
                     /translation="MNVSAPSWLLLIVSLPTASATGRMRIWRALKALGCVALRDGAYL
                     LPARADREKALRDLADECLREGGSAWLMVVQPRSTDEAGSYRQLFDRSDDYAQLRKTW
                     KGANRGLASLATAELARLQRKLQREFDALRAIDFFPGDASAEAEAAWTDFSKRLERAL
                     SPDEPHETRGRIPRLEVGRYQGRTWATRRRLWIDRVASAWLIRRFIDRDARFRWLARP
                     SDCPKSALGFDFDGATFTHVADRVTFETLMASFGLEEDPALVRLAAIVHALDIGGDDP
                     VPEARGFEAVMAGARERLTDDDALLAEMGTVLDSLYAHFQREARRGNDKGNTR"
     gene            2534..>3720
                     /locus_tag="VNU48_07540"
     CDS             2534..>3720
                     /locus_tag="VNU48_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HWI11164.1"
                     /translation="MNDTGSVTEVAQPPSYSLWQLVRYMLALGTWGFGGPVALVGYMY
                     RDLVEKRHWISESDYKEGMALAQLMPGPLAAQLAIYLGYVHYRVRGATLVGLAFVLPS
                     FAMVVAIGAVYSAYGGIAWMQAVFYGVGAAVIGIIAMSAYKLTTKNVGKDRLLWAIFL
                     ASAATTVITKSEIVWIFLGAGVLVWLVRAPPKSGFGGTANSLAAPIVAFFAIDAIDWH
                     RLGQIGAYFAYAGSFVFGSGLAIVPFLYGGVVQEYHWLTDRQFVDAVAVAMITPGPVV
                     ITTGFIGYLVAGFWGAVVAAAGTFVPCYLFTILPAPYFKKHGKRPGIVAFVDGVTAAA
                     IGAISGAVIVIGQRSITDWVTAALALVTVGVLWRLKKLPEPVVVVVAALLGLVLHSLM
                     THP"
BASE COUNT          609 a         1280 c         1286 g          545 t
ORIGIN      
        1 cgcaaccgat gccggatgag agcgttcggc gccgcacgct ggctcaccgg ccggaggaat
       61 cgccgcggct gcgatgtcgg tgtgcggtat gcacactggg gctcatgcaa ccgcaatccc
      121 caacgtagcg ccgtcggacg agcgggtgcc ccgcgtcggc ttccacttcc gacacgctgc
      181 gcacggggaa taacggctca gtgcccgtcg atccgattca gccgcatggc gcgccagagt
      241 gcgaggtcgt aggcgatctt cagcacgccg cagccgatca gcggcaacgc gatccacccg
      301 gcggcgaaca gtgcgccgct cagcgtcggg ctcagcgcgg ccgcgaggct gcgcggcacg
      361 gccgtgaagc tggctgccgc ggcgcgctcc gccggcgtga cgacggccat gacataggcc
      421 gtgcgtgtcg gcacatccat ctgcgacaag gcgctgcgca cgaacagcag cccgagcgcg
      481 atcggcaggc tgggcgcgag cgcggccagc acgaggcaca cattggccgg gatgtgcgtg
      541 aacaccatcg tgttcagcag cccgatgcgc tgagcgacca gcggcgccgc gagctgggac
      601 gccgcgctga gcacgccggc ccagaagaag aacacaccgg cttgcgtgag cgacaacccg
      661 aatcgctgca gcagccacag cgacagcagc gcattgatga ccaacccgcc ggcaaatgcg
      721 tcgacgctga agagcgcggc caatcgcacg acgatggcgt gcgacggacc gagcgcagcg
      781 gtcggcagca gcgcgtcgtc gcggcctgtc tcgcccgacg gcaggcggcg gtagagccag
      841 aacaccgtca gaccgagtgc gccatagacg acgaacatgc cgcccagcgc gtcgagccgc
      901 gcgaccccgg ccacgcgaac cagccagtcc ggcacggcgg cggcgagcgc tccgagcgag
      961 gcgcacaagg ccccgacgac gctgtagcgc gcaaacagcg cggtgcgtgc ggcgccccgg
     1021 gcgcacgccg caagccgcgc gtgctcgagc ggcaggaaca cgctcacatc acccgaactc
     1081 gggttgagcg tgccgacgaa ggcgacgatc agcaagggcc agaacgacgc caggctcgcg
     1141 aatcccaagc ccgtcgcgag catcaacaac gcggccccgc gcagcagccg gcgcgaggcg
     1201 aagcgatgtc cccaggcgcc aaccgcgagc gtggccagcg ccgacccgag catcgtcgcg
     1261 gtgctgacga tgccgacctc gagtgtgccc aacccgatag cgagcagata cgtcggcagc
     1321 agcaaggcca tgtagccgtc ggcgaacgcg cgcagccccc tcgccacgag cagtggcagc
     1381 gcacccgcgt cggcaccgcg gggcaacagc accccggcga ccgcgccgcg cgtcggcagc
     1441 ttcaccggat cggcccaagg tgctgcggct cgacgttcgg ccgtgtcccc aacggaaata
     1501 cgaatgaggg catatcgctt actctacatc accgtatgat tctctacatg aacgtttcag
     1561 cgccgtcgtg gcttctgttg atcgtctcgc tgccgacggc cagtgccacc gggcgcatgc
     1621 gcatctggcg cgcactcaag gccctgggct gcgtggcact gcgcgatggc gcgtatctgc
     1681 tgcccgcgcg agccgatcgc gagaaggcct tgcgggacct cgccgacgag tgcctccggg
     1741 aaggcggcag cgcctggctg atggtcgttc agccacgctc gacggacgaa gccggcagct
     1801 accggcagct gttcgaccgc agcgacgact acgcccaatt gcgcaagacc tggaaggggg
     1861 cgaaccgcgg cctcgcgtcc ctggccactg cggagctcgc ccgattgcag cgcaaactgc
     1921 agcgcgaatt cgatgccctg cgggcgatcg acttcttccc cggcgacgcg agcgcggagg
     1981 ccgaggcggc gtggacggac ttcagcaagc gcctcgaaag ggcgttgtcc cccgacgagc
     2041 cgcacgagac ccggggtcgg attccgcggc tggaggtcgg ccggtaccag gggcgaacct
     2101 gggccacgcg gcgccgcctg tggatcgatc gcgtcgccag cgcctggctg attcgccgct
     2161 tcatcgaccg tgacgcgcgc ttccggtggc tggccaggcc gtcggactgc cccaagagcg
     2221 cgctgggatt cgatttcgat ggcgccacgt tcacgcacgt cgctgaccgc gtgacgttcg
     2281 agacgctgat ggccagcttc ggcctggaag aagacccggc gctggtgcgc ctggcagcga
     2341 tcgtgcatgc actggacatc ggcggtgacg accccgttcc cgaggccagg ggtttcgagg
     2401 ccgtgatggc cggtgcgcgc gaacgcctga ccgacgacga cgcgctgctg gccgagatgg
     2461 gcacggtgct cgattcgctg tacgcgcact tccagcgcga agcccggcgt ggcaatgaca
     2521 aaggaaacac acgatgaacg acacgggctc cgtgacagag gtcgcgcagc cgcccagcta
     2581 ctccttgtgg cagctggtgc gctacatgct cgcgctgggc acctggggct tcggcggccc
     2641 ggtcgcgctg gtcggctaca tgtaccgcga cctggtcgag aagcggcact ggatctcgga
     2701 gagcgactac aaggagggca tggcgctcgc ccagctgatg ccgggcccgt tggccgcgca
     2761 actcgcgatc tacctcggct atgtgcacta ccgtgtgcgc ggtgccacgc tggttgggct
     2821 ggctttcgtg ctgccgtcgt tcgcgatggt cgtcgccatc ggtgccgtgt acagcgccta
     2881 cggtggcatc gcctggatgc aggcggtgtt ctacggcgtc ggcgcggcag tgatcggcat
     2941 catcgcgatg agcgcctaca agctcacgac gaagaacgtc ggcaaggaca ggctgctgtg
     3001 ggcgatcttc ctggccagcg ccgcaacaac ggtgatcacc aagtccgaga tcgtctggat
     3061 ctttctcggc gcgggcgtgc tggtctggct ggtgcgcgcg ccgccgaagt cggggttcgg
     3121 cggtacggcg aacagtcttg ccgcgcccat cgttgcattc ttcgccatcg atgcgatcga
     3181 ctggcacagg ctcggccaga tcggcgcgta cttcgcctac gccggcagct tcgtgttcgg
     3241 cagcggcctg gcgatcgtgc cgtttctgta cggcggcgtc gtgcaggagt accactggct
     3301 caccgaccgg cagttcgtcg atgcggtcgc ggtggcgatg atcacccccg gcccggtggt
     3361 catcaccacc ggcttcatcg gctacctcgt cgccggcttc tggggcgccg tggtcgccgc
     3421 ggcgggcacc ttcgtgccct gctacctgtt caccatcctg ccggcgccgt acttcaagaa
     3481 gcacggcaag cgcccaggca tcgtcgcctt cgtcgacggg gtcacggcgg cggcgatcgg
     3541 cgcgatcagc ggggcggtga tcgtcatcgg ccagcgctcc atcaccgact gggtcaccgc
     3601 ggcactggcg ctcgtcacgg tcggcgtgct gtggcgcctg aagaagctcc cggagcccgt
     3661 cgtcgtcgtc gtcgcggcat tgctgggtct ggtgctgcac tcgttgatga cccacccctg
//
LOCUS       DATMJB010000348         4725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_68779, whole genome shotgun sequence.
ACCESSION   DATMJB010000348 DATMJB010000000
VERSION     DATMJB010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68779"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(323..841)
                     /locus_tag="VNU48_07545"
     CDS             complement(323..841)
                     /locus_tag="VNU48_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWI11165.1"
                     /translation="MKLHPLTTRRRMLQGSAGVALALLAGRALPAIATATAAPDFTLR
                     MLGGPNLRLAEQRGRVVMINFWATWCGPCRQEMPQLSKLYDKYRAAGFVLLGVNVDDD
                     VRNATAVATKLGVSFPVLLDTDKTVSKLYDLSTMPSTVLVDRDGQVRYLHRGYLAGYE
                     DTYEKQVRELLK"
     gene            complement(857..1495)
                     /locus_tag="VNU48_07550"
     CDS             complement(857..1495)
                     /locus_tag="VNU48_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HWI11166.1"
                     /translation="MNHKTALLTIAMLAIGANAFAADAPAKPTDQVVVPQVDRRDISV
                     PRLPSNDFEIGAFVGTYATQNFGSSAVGGLRLGYHVTEDFFVEGVYAQTKVSDESFRQ
                     VLPGGVFPDEKEKLSYYNLSVGYNVLPGEVFIGGKRAKPSAFYLIGGVGSTKFDQQRR
                     PTYNVGAGLRVFLGDRIALQVDARDHIFSLDLLGKRQSTQNLEFSAGITAFF"
     gene            complement(1504..2304)
                     /locus_tag="VNU48_07555"
     CDS             complement(1504..2304)
                     /locus_tag="VNU48_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11167.1"
                     /translation="MKTTDWKRRARRDRALAAIALFALLLVGATVARADDVPPPAAPT
                     TERLQITDPYIELHTGPGRGFPVFFVASRTQWIEIELRHTDWYKVRTDDGKVGWVNRT
                     QLETTLTAAGGAKSFRDVLLDDYLSRRVQLGAAWGKFKSEPMLKLWTSYRLSETLSVE
                     GTLGQVQGVFSGTDFWHLDLMAEPWSDRRLSPFFAIGVGKFKNYPNLSLVGAQTTDAK
                     LANASVGLRYHLSDRFVVRADYSLYTAFVADTHSTEYRAFTAGLSFFF"
     gene            complement(2301..2912)
                     /locus_tag="VNU48_07560"
     CDS             complement(2301..2912)
                     /locus_tag="VNU48_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11168.1"
                     /translation="MKPALIPALRVAAIAASALLASCGGGSSNPLDNPTTVVNGAAPS
                     GQRLSFAYFQKCINPILIAQLPVPNGSGTNTCAGSGCHDTTTGTGGAFRIVPGAQALD
                     VADPTLTPDAIRASDMYKNFYSAQGEVVFGSTTQSRILAKPLLQGVLHGGGLIFASAA
                     DPNAKLIAYWISHPAPLGQDEFSTATWAMFTPADPNTGTCNTQ"
     gene            complement(2921..>4725)
                     /locus_tag="VNU48_07565"
     CDS             complement(2921..>4725)
                     /locus_tag="VNU48_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11169.1"
                     /translation="RLVADAQADELQNNVPPSYAVYMFDPKPQTFLIVAAPPKGFMYT
                     HPVALLPRAEPNATDPTAVDATLAAQNLGLLEVRSVYDTDGLGRMGDPVMAAADLPAG
                     CTSAIAKTAPTDPNDTRAQVADIVRIKNPADPAYGCAPARFVRAVRAIAPPQGMTGMR
                     SAIGETEFEMQQILGYAPIEPDGSFKLQVPADTPIALAVIDAQGRAFQTHTNWIQVRP
                     GERRTCDGCHSPRRGGSLNSGAIVNSVPAALLPAMASAHLSGETMAATRTRLDPSALQ
                     LAADLLYRDVWADTSKTGVTAHAPITIRYTGNASAANDLATPAPVNGIVNYPDNIQPL
                     WTRDRGANTCVACHNNPDKLDLSATLAGSGRLSSYDELMIGDPLIDATTGLPQTRIEE
                     GVLVIARGPALVDTAASEGEALGLARKSRLAEILWGQALMSGSDARAAHPNPPGTAPD
                     HSTLLNAAEKRLVAEWIDTGGKYYNDPFDAANGVRQVNGLSEDSFIASVYPILMNTCA
                     AGCHQAIGSSTSAVPVGTSFRNNRFVLTGDPDGDYNVTLTMISNACNAPSNYLLSKPS
                     TVPHPTGAVGATSAVLPVGSANYNAIASWIASGC"
BASE COUNT          666 a         1559 c         1726 g          774 t
ORIGIN      
        1 ctggttgacg ccttcgactt tcagcatgtc aatcgctcca acccgcgcac gccgcgacgc
       61 gccgcctgcc ccaccggccc gatctgctcc accgtgcagg ccgatgaccg gcgcacaaca
      121 cctcggtctg ctgccgaggg agtcgggtgg gcaacgcagc gaagtaaagg agggacgggc
      181 gcagcccgtt ccgggggaca tgagcggagt tgtccacccg actctctcgg ctcgcgccga
      241 ccgtcaaagg gcgcacgctc gcttgcgaac gaccgcaacg ggccgcaagc agacacacca
      301 tcacaactcc acaccgctgg cttcacttca gcaactcccg gacctgcttc tcgtaggtgt
      361 cttcgtagcc ggcgaggtac ccacggtgca gatagcgcac ctggccatcg cgatcgacca
      421 gcacggtcga cggcatcgtg ctcaggtcgt agagcttgct gaccgtcttg tcggtgtcca
      481 gcagcacggg aaagctcacg ccgagcttgg tggccaccgc agtcgcgttg cgcacgtcgt
      541 cgtcgacgtt cacgccgagc agcacgaagc ccgcggcgcg gtacttgtcg tagagcttgc
      601 tgagctgcgg catctcctgc cggcacgggc cgcaccaggt ggcccagaag ttgatcatca
      661 cgacacggcc gcgctgctcg gccaggcgca ggttcggacc acccagcatg cgcagcgtga
      721 agtcgggcgc ggccgtcgcg gtcgcgatcg ccggcagcgc gcgaccggcc agcagcgcga
      781 gcgcgacgcc ggccgagccc tgcaacatcc ggcgacgcgt ggtgagggga tggagtttca
      841 tgcgtatccg tccggctcag aagaaggccg tgatgccggc gctgaactcg aggttctgcg
      901 tgctctggcg cttgccgagc aggtcgagcg agaagatgtg atcgcgcgca tcgacctgca
      961 gcgcgatgcg gtcgccgagg aacacgcgca gcccggcgcc gacgttgtag gtcggccggc
     1021 gctgctggtc gaacttggtg ctgccgacgc cgccgatcag gtagaaggcc gagggcttgg
     1081 cgcgcttgcc gccgatgaac acctcgccgg gcagcacgtt gtagccgacc gagaggttgt
     1141 agtagctcag cttctccttc tcgtccggaa acacgccgcc gggcagcacc tgacggaagg
     1201 attcgtcgct gaccttggtc tgcgcgtaga cgccttcgac gaagaagtcc tcggtgacgt
     1261 ggtagcccag gcgcaacccg ccgaccgcgc tggagccgaa gttctgcgtc gcgtaggtgc
     1321 cgacgaaggc gccgatctcg aagtcgttcg acggcagccg cggcacgctg atgtcgcgcc
     1381 ggtcgacctg cggcacgacg acctggtcgg tgggcttcgc gggcgcgtcg gcggcgaagg
     1441 cgttggcgcc gatcgcgagc atcgcgatcg tcagaagtgc agtcttgtgg ttcatggcgg
     1501 tggtcagaag aagaacgaga ggcccgcggt gaacgcgcgg tattcggtgc tgtgcgtgtc
     1561 ggcgacgaac gcggtgtaga gcgagtagtc ggcgcgcacg acgaagcggt cgctcaggtg
     1621 gtagcgcagc ccgacgctcg cgttggcgag cttcgcgtcg gtggtctgcg cgcccaccag
     1681 gctgaggttc gggtagttct tgaacttgcc cacgccgatc gcgaagaacg gcgagagccg
     1741 ccggtccgac cagggctcgg ccatcaggtc gaggtgccag aaatcggtgc ccgagaacac
     1801 gccctgcacc tggccgagcg tgccctcgac gctgagcgtc tcggacagcc ggtagctggt
     1861 ccacagcttg agcatcggct cggacttgaa ctttccccag gccgcgccga gctgcacgcg
     1921 ccggctcagg tagtcgtcga gcagcacgtc gcggaagctc ttcgcgccgc ctgccgccgt
     1981 cagcgtggtc tcgagttgcg tccgattcac ccagccgacc ttgccgtcgt cggtgcgcac
     2041 cttgtaccag tcggtgtggc gcagctcgat ctcgatccac tgcgtgcgcg acgcgacgaa
     2101 gaagaccggg aagccgcgcc ccgggccggt gtgcagctcg atgtacgggt cggtgatctg
     2161 cagccgctcg gtggtgggcg cggctggcgg cggcacgtcg tcggcgcgcg cgacggtcgc
     2221 gccgacgagc agcaatgcga acagcgcgat cgcagccagc gcgcgatcgc gccgggcgcg
     2281 gcgtttccag tcggtggttt tcattgcgtg ttgcacgtgc cggtgttcgg gtcggcgggc
     2341 gtgaacatcg cccaggtcgc ggtgctgaac tcgtcctgcc cgagcggcgc cgggtggctg
     2401 atccagtagg cgatcagctt ggcgttcggg tcggcggcgc tcgcgaagat caggccgcca
     2461 ccgtgcaaca cgccctgcag cagcggcttg gcgaggatgc ggctctgcgt cgtcgagccg
     2521 aacacgacct cgccctgcgc cgaatagaag ttcttgtaca tgtcgctggc gcggatcgcg
     2581 tcgggtgtca gcgtcgggtc ggccacgtcc agcgcctgcg cgccggggac gatccggaat
     2641 gctccgccag tgccggtggt ggtgtcgtgg cagcccgagc cggcgcaggt gttggtgccg
     2701 ctgccgttcg ggaccggcaa ctgcgcgatc aggatcgggt tgatgcactt ctggaagtac
     2761 gcgaacgaca ggcgctggcc cgaaggcgcg gcgccgttca cgaccgtcgt cgggttgtcg
     2821 agcgggttcg acgaaccgcc gccgcaactc gcgagcagcg cgctcgcggc gatcgcggcg
     2881 acgcgcagcg cggggatcag tgcgggcttc atgcgcgtca tcagcagccc gacgcgatcc
     2941 agctcgcgat cgcgttgtag ttcgcgctgc ccaccggcag caccgcgctc gtcgcgccga
     3001 cggcgccggt cggatgcggc acggtcgagg gcttgctcag caggtagttc gatggcgcgt
     3061 tgcaggcgtt cgagatcatc gtcagcgtca cgttgtagtc gccgtccggg tcgccggtga
     3121 gcacgaagcg gttgttgcgg aacgaggtgc cgaccggcac cgccgacgtg ctgctgccga
     3181 tcgcctggtg gcagccggcg gcgcaggtgt tcatcaggat cgggtagacg ctggcgatga
     3241 acgagtcctc gctcaggcca ttgacctggc gcacgccatt ggcagcatcg aacgggtcgt
     3301 tgtagtactt gccgccggtg tcgatccatt cggccaccag gcgcttctcg gccgcgttca
     3361 gcagcgtcga gtgatccggt gcggtgcccg gcgggttcgg gtgcgcggcg cgcgcgtcgc
     3421 tgcccgacat cagcgcctgg ccccagagga tttcggcgag ccggctcttg cgcgccaggc
     3481 ccagcgcctc gccttcgctg gccgcggtgt cgaccagcgc cggcccgcgc gcgatcacca
     3541 gcacgccttc ctcgatgcgc gtctgcggca ggccggtggt cgcgtcgatc agcgggtcgc
     3601 cgatcatcag ctcgtcgtag gaactcagcc gcccggagcc ggccagcgtc gcgctcaggt
     3661 cgagcttgtc ggggttgttg tggcacgcga cgcaggtgtt cgcgccgcgg tcgcgcgtcc
     3721 acagcggctg gatgttgtcg gggtagttga cgatgccgtt gaccggcgcc ggcgtcgcca
     3781 ggtcgttggc cgcgctcgcg ttgccggtgt agcggatcgt gatcggcgcg tgggcggtga
     3841 cgccggtctt gctggtgtcg gcccagacgt cgcggtacag caggtcggcg gcgagttgca
     3901 gcgcactcgg gtcgagccgc gtgcgcgtcg ccgccatcgt ctcgcccgac aggtgcgcgc
     3961 tcgccatcgc cggcagcagc gccgccggca cgctgttgac gatcgcgccc gagttcagcg
     4021 agccgccacg gcgcgggctg tggcagccgt cgcaggtgcg gcgctcgccg gggcgcacct
     4081 ggatccagtt ggtgtgggtc tggaacgcgc ggccttgcgc gtcgatgacc gccagggcga
     4141 tcggcgtgtc ggccggcacc tgcagcttga acgagccgtc gggctcgatc ggcgcgtagc
     4201 cgaggatctg ctgcatctcg aactcggtct cgccgatcgc gctgcgcatg ccggtcatgc
     4261 cctgcggcgg cgcgatcgca cgcaccgcgc gcacgaagcg cgccggcgcg cagccgtagg
     4321 ccgggtcggc cgggttcttg atgcgcacga tgtcggcgac ctgcgcgcgc gtgtcgttcg
     4381 ggtcggtcgg cgctgtcttc gcgatcgcgc tcgtgcagcc ggcgggcagg tcggcggcgg
     4441 ccatcaccgg gtcgcccatg cggcccaggc cgtcggtgtc gtagacgctg cgcacttcga
     4501 gcaggccgag gttctgcgcc gcgagcgtgg cgtcgaccgc ggtcgggtcg gtcgcgttcg
     4561 gctcggcgcg cggcaacagc gcgaccgggt gcgtgtacat gaagcccttc ggcggcgcgg
     4621 cgacgatcaa gaaggtctgc ggcttcgggt cgaacatgta gaccgcgtac gagggcggca
     4681 cgttgttctg cagctcgtcg gcctgggcat cggcgacgag gcggt
//
LOCUS       DATMJB010000349         2447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_478172, whole genome shotgun sequence.
ACCESSION   DATMJB010000349 DATMJB010000000
VERSION     DATMJB010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478172"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..89)
                     /locus_tag="VNU48_07570"
     CDS             complement(<1..89)
                     /locus_tag="VNU48_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWI11170.1"
                     /translation="MSLKDQITEDMKTAMRAKDAPRLLTIRGLL"
     gene            complement(180..392)
                     /gene="rpsU"
                     /locus_tag="VNU48_07575"
     CDS             complement(180..392)
                     /gene="rpsU"
                     /locus_tag="VNU48_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWI11171.1"
                     /translation="MTTIRVKENEPFDVALRRFKRTIEKLGLLTDLRAREFYEKPTAE
                     RKRKKAAAVKRHFKRVRSMQLPKKLF"
     gene            complement(585..1787)
                     /locus_tag="VNU48_07580"
     CDS             complement(585..1787)
                     /locus_tag="VNU48_07580"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWI11172.1"
                     /translation="MSFDAVIVGAGAAGLHCAAIAGQRGLHVLLIDHAGKVAEKIRIS
                     GGGRCNFTNRDTTPANFLSANPPFCRSALARYTPADFIALLQRHGVGWHEKHKGQLFC
                     DEGSEAVIAMLLRECAQGSVERWQPCAVQAVRHGAAGFELDTDRGSVRARQLVIATGG
                     LSIPKIGASDFGYRLARQFGHAIEETRPALVPLTFDPVSWEPFAALSGLSLEAGIETG
                     DGKARTRFVEDLLFTHRGLSGPAVLQISSFWRAGTPIRLDLAPGRDLAAELLRAKRDS
                     RRKLANELAELLPKRLADTWLLPTPALGERTLPEQRDRDLAQLAARLQRWELKPQATE
                     GYRKAEVTAGGVDTRELSSQTMQSQRVEGLYFIGEVVDVTGWLGGYNFQWAWASAAAC
                     GRALGRAG"
     gene            complement(1784..>2447)
                     /locus_tag="VNU48_07585"
     CDS             complement(1784..>2447)
                     /locus_tag="VNU48_07585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWI11173.1"
                     /translation="WQTDFHHFIVNHMVVGFVLLATNLLVHKLFGWAAQDGIRGFVQN
                     LNFFVALFLIVLVADLVQYWTHRAYHEVPLLWRLHAVHHSVKSMDWLAGSRQHILELI
                     ITRTLVLAPIYVLAFSKEVIDAYIIVVGFQAVFNHANVSVRLGPLRYVIVTPNFHHWH
                     HSQDDDAIDRNYAAHFAFIDHAFGTAVKSEREWPEHYGVVGDYVPNGFFRQLAFPFVW
                     KG"
BASE COUNT          395 a          814 c          839 g          399 t
ORIGIN      
        1 agcaggccgc ggatcgtcag caggcgcggc gcgtccttgg cgcgcatcgc ggtcttcatg
       61 tcttcggtga tctggtcctt gaggctcatg cgaactccgg tctggaaacg aagaagcccg
      121 cgggtcgcgg gcttcctggg caaggacaag ccccgttcgg ggggctcagc gccgcgagat
      181 cagaacagct tcttcggcag ctgcatgctg cgaacgcgct tgaagtgacg cttcacggcc
      241 gccgccttct tgcgcttgcg ttcggcggtg ggcttctcgt agaactcgcg ggcacgcagg
      301 tcggtcagca ggccgagctt ctcgatggtg cgcttgaagc ggcgcagggc cacgtcgaat
      361 ggctcgtttt ctttgacgcg aatcgtagtc atgtaaaggg ttgatcctga aataaaggtg
      421 cgctcggtgt cggaaggcgc tcggtgaacc gcagggtccg cagtccccgc tctagagcgt
      481 cccgccaatc gcaacgcgtg gttttgccgg caaagactat cgattatagc ccgggtgggg
      541 aacgcccgca atctatccga ccacaccacg cctgatgctg cagctcagcc cgcgcggccg
      601 agcgcccggc cgcaggcggc cgcactcgcc cacgcccact ggaagttgta gccgccgagc
      661 cagccggtca cgtcgaccac ctcgccgatg aagtacagcc cctcaacgcg ctggctctgc
      721 atcgtctgtg agctgagctc gcgtgtgtcg acgccgccgg ccgtgacctc cgccttgcgg
      781 tagccctcgg tcgcctgcgg cttcagctcc cagcgctgca gtcgtgcggc cagctgtgcg
      841 aggtcgcgat cgcgctgctc aggcagcgtg cgctcgccaa gcgccggcgt cggcagcagc
      901 caggtgtcgg cgaggcgctt cggcaacagc tcggcgagct cgttcgcaag cttgcggcgc
      961 gagtcgcgct tggccctcag cagttcggcg gccagatcgc gacccggtgc caggtcgagc
     1021 cggatcggcg tgccggcgcg ccagaagctg gagatctgca acaccgccgg gccgctcagg
     1081 ccgcggtgcg tgaacagcag gtcctcgacg aagcgcgtgc gcgccttgcc gtcgccggtc
     1141 tcgatgccgg cttccagcga caggccggac agtgccgcga acggctccca gctcaccggg
     1201 tcgaaggtca gtggcaccag cgcgggacgg gtctcctcga tggcgtggcc gaactggcgt
     1261 gcaagccggt agccgaagtc gctcgccccg atcttcggga tcgacagacc accggtcgcg
     1321 atcaccaact gcctggcgcg gacgctgccg cgatcggtgt cgagctcgaa gccggcggcg
     1381 ccgtgccgca ccgcctgcac ggcgcagggc tgccagcgct cgacgctgcc ctgcgcgcat
     1441 tcgcgcagca gcatcgcgat gacggcctcg ctgccctcgt cgcagaacag ctgccccttg
     1501 tgcttctcgt gccagccgac gccgtggcgc tgcagcagcg cgatgaagtc ggccggcgtg
     1561 tagcgcgcca gcgccgagcg gcagaagggc gggttggcgg acaggaagtt cgccggcgtc
     1621 gtgtcgcgat tcgtgaagtt gcagcgcccg ccgccggaaa tgcggatctt ttcggcgacc
     1681 ttcccggcat ggtcgatcag cagcacgtgc agcccgcgtt ggccggcgat cgcggcgcag
     1741 tgcagcccgg ccgcgccggc gccgacgatg accgcgtcga agctcagccc ttccacacga
     1801 acgggaacgc cagctgcctg aagaagccgt tggggacgta gtcgccgacc acgccgtagt
     1861 gctcgggcca ttcgcgctcg gacttcaccg cggtgccgaa cgcgtggtcg atgaacgcga
     1921 aatgggccgc gtagttgcgg tcgatcgcgt cgtcgtcctg cgagtggtgc cagtggtgga
     1981 agttgggcgt cacgatcacg tagcgcagcg gcccgagccg cacgctgaca ttcgcgtggt
     2041 tgaacaccgc ctggaaaccg acgacgatga tgtaggcgtc gatcacttcc ttgctgaagg
     2101 ccagcacata gatcggtgcc agcaccagcg tgcgcgtgat gatcagctcg aggatgtgct
     2161 ggcgcgagcc ggccagccag tccatgctct tgacgctgtg gtgcaccgcg tgcagccgcc
     2221 acagcagcgg cacctcgtgg taggcgcggt gcgtccagta ctgcaccagg tcggcgacca
     2281 gcacgatcag gaacagtgcg acgaagaagt tcaggttctg cacgaagccg cggatgccgt
     2341 cctgcgcggc ccagccgaac agcttgtgca cgagcaggtt cgtcgccagc agcacgaagc
     2401 caaccaccat gtggttgacg atgaagtggt gaaagtcggt ctgccac
//
LOCUS       DATMJB010000350         5617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_476033, whole genome shotgun sequence.
ACCESSION   DATMJB010000350 DATMJB010000000
VERSION     DATMJB010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476033"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="VNU48_07590"
     CDS             complement(<1..1307)
                     /locus_tag="VNU48_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWI11174.1"
                     /translation="MHLPSIATGKRFTLPRPLGSADALLLARFAHAQAAQRKIIAIVT
                     AEPADTQRLEAELPFFAPGLRIAVFPDWETLPYDTFSPHQDLISERLATLWRIQQGEI
                     DVVLMPASTALVRLAPPSFLAGYTFHFKQKEKLNEAALKAQLTLAGYSHVSQVVSPGE
                     YAVRGGLIDLFPMGSLVPYRVDLFGDEVDSIRTFDPDTQRSLYPVPEVRLLPGREFPM
                     DEDARTRFRARWREQMEGDPSKVRLYKDIGTGLASGGIEYYLPLFFDATATIFDYLGT
                     DATLVLHGEIDAALQKFWTDTRERHRFLQADRERPILPPEELFLKPEEFFAHCNHHAQ
                     LALRGSEEVAWARALPDLGVDRGAPEPLQRLKAQVATTKYRVLIVAETEGRRESLLEL
                     LRDSKIEPPSVATLAEFQAGDEHFAIAVAPLAQGFFWFEPDDAV"
     gene            1406..2113
                     /gene="ispD"
                     /locus_tag="VNU48_07595"
     CDS             1406..2113
                     /gene="ispD"
                     /locus_tag="VNU48_07595"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWI11175.1"
                     /translation="MTLAAPLDQPRLYALVPCAGVGERAGTKGPKQYAMLGTHSLLAH
                     TLAALGRVARIDSVLVLLSPADTEHAAHLAGSDAGIAHCGGATRAQTVRNGLDELLAR
                     GARAQDWVLVHDAARCLVRPEWIDALIDACLTDPVGGLLALPLADTLKAERDGRVAAT
                     IDRRAKWQAQTPQMFRLGLLRDALAAAGAEVTDEASAIEAAGHAPKLVPGALENFKIT
                     WPADFALAERLLRTRTT"
     gene            2110..2613
                     /gene="ispF"
                     /locus_tag="VNU48_07600"
     CDS             2110..2613
                     /gene="ispF"
                     /locus_tag="VNU48_07600"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWI11176.1"
                     /translation="MNAPRIKAPLWRIGEGWDMHALVAGRRLVLGGVDIPHTHGLMGH
                     SDADALCHAITDALFGAAAMGDIGFHFPDTDPQYQGADSLALLAECARRVRAAGWLIG
                     NVDSTIIAQAPKMAPHILAMRQRVAQAIGVAVDQVSVKAKTAEKMGPVGLGQAIEARA
                     VCLIAKG"
     gene            complement(2653..4719)
                     /locus_tag="VNU48_07605"
     CDS             complement(2653..4719)
                     /locus_tag="VNU48_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWI11177.1"
                     /translation="MKFRLPGSVPFRLRVFFRGAFAALALATLALAVNVLQDEKQRSH
                     RSYADGLHRNQAQIAARLRHPTGQLVLLNPGIAERTAAPLHPLVLPFSAIDFDDRIKA
                     LQAVEMAGCLVQYPNASTLCAGVGNNPYAGAFVYLVGSVASAELVAHEPGDLDLTAAH
                     RVRVELEMRGQTFRWVAPFEAIGDASSSLRRGRLTGFVADQPVTPSTRPVRDFRGWLW
                     QEGRCIEAAADPAHCLKRSFVSVRLPVEIFRDALFQKPLVWPPPDLAQMHVRLQLLPP
                     GDAAPLFDSDAAGATWPFSLADLRALLLPGELLRVRKLGAGADAPPLELFSLRGATAT
                     DEPISPWLAQLIRRLPVEGYDAPIEARETIATPLGRYELVLTGDVRSVNRSLATVATR
                     VSWFVGAMLLAIALTWLAIELRIIRRITLLTRRAASVSTGVRAADGLAQVDLSDLRGS
                     DELGVLAQGLQDLLQRVNDDVRREHIRAEQEKDMWHAVGHEIMSPLQSLLALHGAPDD
                     ASHRYVSRMQQAVRVLYGHASPSEALQSTTLQLQPLDLNAFLGNVARNAPYSGVEHVV
                     YSALGQAVPVRAEEYALEDVVTHVLRNADRYRSVGSPIRIGLELADGEACVQIHNDGA
                     PIAAGLIDSIFEYGVCDPADAPTSGHRGQGLFVAKTYMAKMGGTIVAANVADGVRFTL
                     SLPLAG"
     gene            complement(4730..5428)
                     /locus_tag="VNU48_07610"
     CDS             complement(4730..5428)
                     /locus_tag="VNU48_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI11178.1"
                     /translation="MPKVAVIEDDVPTSNQLRGWIEAARPGIEVHQWFSRDDAESALA
                     REHYDVVVLDIELGRERHAGVAIINTINKQRGTPVLVVSAMPAAIYRSIMKALDAWDY
                     LQKTTFEEADFIDTFLEILRAAKSADAAPARPAATAELSLDPLRQSSPIWRGQRVNLP
                     LTAQRILAALHQRRGEIVSYEELFLVVKSGRNRDNIRKHVGTIRDALREIEPAFDAIE
                     NVPMRGFRWTKPAS"
     gene            5427..>5617
                     /locus_tag="VNU48_07615"
     CDS             5427..>5617
                     /locus_tag="VNU48_07615"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI11179.1"
                     /translation="MARIIESGLPVPEPFEAAYAASKSALMVFGDSLRVELDGAAIGV
                     TILNPAVVDTRFFDRRGVP"
BASE COUNT          824 a         1927 c         2029 g          837 t
ORIGIN      
        1 acggcgtcat ccggctcgaa ccagaagaag ccctgcgcga gcggcgcgac cgcgatcgcg
       61 aagtgctcgt caccggcctg gaactcggcc agcgtcgcca cgctcggcgg ctcgatcttg
      121 ctgtcacgca gcagctcgag caggctctcg cgccggcctt cggtctcggc gacgatcagc
      181 acccggtact tcgtcgtcgc gacctgcgcc ttcaggcgct gcagcggctc cggggcgcca
      241 cggtcgacgc ccaggtcggg cagcgcgcgc gcccatgcca cttcttcgct gccgcgcagc
      301 gcgagctgtg catggtggtt gcagtgcgcg aagaactctt ccggcttcag gaacagctct
      361 tcgggcggca ggatcggccg ctcgcggtcg gcctgcagga agcggtggcg ctcgcgcgtg
      421 tcggtccaga acttctgcag cgccgcgtcg atctcgccgt gcagcacgag cgttgcgtcg
      481 gtgccgaggt agtcgaagat cgtcgcggtg gcgtcgaaga acagcggcag gtagtactcg
      541 atgccgccgc tggccagccc ggtgccgatg tccttgtaca ggcgcacctt gctcgggtcg
      601 ccctccatct gctcgcgcca gcgcgcccgg aaacgcgtgc gcgcgtcctc gtccatcggg
      661 aactcgcgcc ccggcagcag ccgcacctcg ggcaccgggt acaggctgcg ctgggtgtcg
      721 gggtcgaagg tgcggatcga gtcgacctcg tcgccgaaca ggtcgacgcg gtacggcacc
      781 aaagagccca tcgggaacag gtcgatcagc ccgccgcgca ccgcatattc gccgggcgag
      841 accacctggc tcacgtggct gtagccggcc agtgtgagct gggccttcag cgcggcctcg
      901 ttgagctttt ccttctgctt gaagtggaag gtgtagccgg ccaggaagct cggcggcgcc
      961 agccgcacca gcgcggtcga ggccggcatc agcacgacgt cgatctcgcc ttgctggatg
     1021 cgccacaagg tcgccagtcg ctcggagatc aggtcctggt gcggcgagaa ggtgtcgtag
     1081 ggcagcgtct cccaatcggg gaacacggcg atgcgcaggc ccggcgcgaa gaagggcagt
     1141 tcggcctcga ggcgctgcgt gtcggccggc tcggcggtga cgatcgcgat gatcttgcgc
     1201 tgcgccgcct gcgcgtgcgc gaagcgggcc agcagcaagg cgtcggcgga gccgagcggg
     1261 cggggcagcg tgaagcgttt tccggtggcg atcgagggca ggtgcatgaa ggcgtgggac
     1321 acacaaagca aaacgccccg cgggccttgc tgacgtggcg tcccccgggg cggcaatgag
     1381 ggccgattat agaattcaag ggccgatgac gcttgccgcc ccccttgacc aaccccgcct
     1441 gtacgcgctc gttccctgtg ccggcgtcgg cgaacgtgcg ggaaccaagg ggccgaagca
     1501 gtacgcgatg ctcggaacgc acagcctgct cgcgcacacg ctggcagcgc tcgggcgcgt
     1561 cgcgcggatc gattcggtgc tggtgctgct gagcccggcc gacaccgagc acgccgcgca
     1621 cctggccggc agcgatgcag gaatcgcgca ctgcggcggc gccacgcgcg cccagaccgt
     1681 gcgcaatggg ctcgatgaac tgctggcacg cggcgctcgc gcacaggact gggtgctggt
     1741 gcacgacgcc gcacgctgcc tggtgcgccc ggagtggatc gacgcgctga tcgacgcctg
     1801 cctgaccgac ccggtcggcg gcctgctcgc gctgcccttg gccgacacgc tgaaggccga
     1861 gcgcgacggc cgcgtcgcgg cgacgatcga ccggcgcgcg aagtggcagg cacagacgcc
     1921 gcagatgttt cgcctggggc tgctgcgcga cgcgctcgcc gcggccggcg ccgaggtgac
     1981 cgacgaggcg agcgcgatcg aggccgccgg ccacgcgccg aagctggtgc cgggtgcgct
     2041 cgagaacttc aagatcacct ggccggccga tttcgcgctg gccgagcggc tgctgaggac
     2101 tcgcacgaca tgaacgctcc ccggatcaag gcaccgctgt ggcgcatcgg cgaaggctgg
     2161 gacatgcacg cgctggtggc tggccgccgg ctcgtgctcg gcggtgtcga cattcctcac
     2221 acgcacgggc tgatgggtca ttcggatgcc gacgcgctgt gccatgcgat caccgacgcg
     2281 ctgttcggtg ccgccgcgat gggcgacatc ggctttcact ttcccgacac cgacccgcag
     2341 taccagggcg ccgactcgct cgcgctgctc gcggagtgcg cgcgccgcgt gcgcgcggcc
     2401 ggttggctga tcggcaatgt cgacagcacc atcatcgcgc aggcaccgaa gatggcgccg
     2461 cacatcctgg cgatgcgcca gcgcgtcgcg caggcgatcg gcgtcgcggt cgatcaggtc
     2521 agcgtgaagg cgaagacggc cgagaagatg gggccggtcg gcctcgggca ggcgatcgag
     2581 gcgcgcgcgg tgtgcctgat cgcgaaaggt tagcgtcggc cgtcggcgcg agccgcggcc
     2641 gatgcgaggt gctcatccgg ccagcggcag gctcaaggtg aatcggacgc catcggcgac
     2701 gttggcggcg acgatggtcc cgcccatctt cgccatgtag gtcttcgcca cgaacaggcc
     2761 ctggccgcga tggcccgagg tcggcgcgtc ggccgggtcg cagacgccgt actcgaagat
     2821 cgaatcgatc agcccggccg cgatcggcgc gccgtcgttg tggatctgca cgcaggcctc
     2881 gccgtcggcc agctccaggc cgatgcggat cggcgagccg acgctgcggt agcggtcggc
     2941 gttgcgcagc acgtgggtca cgacgtcctc gagcgcgtac tcctcggcgc gcaccggcac
     3001 cgcctgcccg agcgcgctgt agaccacgtg ttcgacgccg ctgtacggcg cgttgcgcgc
     3061 cacgttgccg aggaaggcat tcaggtcgag cggctgcagt tgcagcgtcg tcgactgcag
     3121 cgcctcgctc ggcgaggcgt ggccgtacag cacccgcacc gcctgctgca tgcggctgac
     3181 gtagcggtgg ctggcatcgt cgggcgcgcc gtgcagcgcc agcagcgatt gcagcggcga
     3241 catgatctcg tgcccgaccg cgtgccacat gtccttctcc tgctcggcgc ggatgtgttc
     3301 gcgcctgaca tcgtcgttga cccgctgcag caagtcctgc aggccttggg ccagcacgcc
     3361 gagttcgtcg ctgccgcgca gatcggacag gtcgacctgc gcgagcccgt cggcggcgcg
     3421 cacgccggtc gacacgctcg ccgcacgcct ggtcagcagc gtgatgcggc ggatgatgcg
     3481 cagctcgatc gcgagccagg tcagcgcgat cgccagcagc atcgcgccga cgaaccatga
     3541 cacgcgcgtc gcgaccgtcg cgaggctgcg gttcacgctg cgcacgtcgc cggtcagcac
     3601 gagctcgtag cgcccgagcg gcgtggcgat cgtctcgcgt gcctcgatcg gcgcgtcgta
     3661 gccctcgacc ggcaggcgcc ggatcagctg ggcgagccag ggcgagatcg gctcgtccgt
     3721 cgcggtggcg ccgcgcaggc tgaacagttc gagtggcggc gcgtctgctc cggcgccgag
     3781 cttgcgaacg cgcagcagct cgccgggcag cagcaacgcc cgcaggtcgg cgagcgagaa
     3841 cggccaggtc gcgccggcgg cgtcgctgtc gaacagcggc gccgcatcgc cgggtggcag
     3901 cagttgcagg cgcacgtgca tctgcgccag gtccgggggc ggccagacca gcggcttttg
     3961 gaacagcgcg tcgcggaaga tctcgaccgg cagccgcacc gacacgaacg agcgcttcag
     4021 gcaatgggcc gggtcggcgg cggcctcgat gcagcgcccc tcctgccaca gccaaccgcg
     4081 gaagtcgcgc accggccggg tcgagggcgt gaccggctga tcggcgacga agccggtcag
     4141 gcgaccgcgc cgcaacgacg acgaggcgtc gccgatcgcc tcgaacggtg cgacccagcg
     4201 aaaggtctgg ccgcgcatct cgagctcgac gcgcacccgg tgcgccgccg tcaggtcgag
     4261 gtcgccgggt tcgtgcgcga cgagttcggc actcgcgacg ctgcccacca ggtagacgaa
     4321 cgcgccggca tacgggttgt tgccgacgcc cgcacacaag gtcgaggcgt tcgggtactg
     4381 gaccagacaa ccggccatct cgaccgcctg cagtgccttg atccggtcgt cgaaatcgat
     4441 cgccgagaac ggcagcacca gcgggtgcag cggcgccgca gtgcgctcgg cgatgccggg
     4501 gttgagcagc acgagctggc ccgtcgggtg gcgcagccgt gccgcgatct gcgcctggtt
     4561 cctgtgcagg ccgtcggcgt agctgcggtg gctgcgctgc ttctcgtcct gcagcacgtt
     4621 gacggccagc gccagtgtgg ccagcgccag cgccgcgaac gcgccgcgga agaacacgcg
     4681 cagccgaaac ggcacgctgc cgggcaggcg gaacttcacg cgtgcgccgt catgaggcgg
     4741 gcttggtcca gcggaagccg cgcatcggca cgttctcgat cgcgtcgaac gccggctcga
     4801 tctcgcgcag cgcatcgcga atcgtgccga cgtgcttgcg gatgttgtcg cggttgcgcc
     4861 cgctcttgac gaccaggaac agctcctcgt acgagacgat ctcgccgcgc cgctggtgca
     4921 gcgcggccag gatgcgctgc gcggtcagcg gcagattgac gcgctggccg cgccagatcg
     4981 gcgagctctg gcgcagcggg tcgagcgaca gttccgccgt cgcagcgggc cgggccggcg
     5041 ccgcgtcggc gctcttcgcg gcgcgcagga tctcgaggaa ggtgtcgatg aagtcggctt
     5101 cctcgaaggt cgtcttctgc aggtagtccc aggcgtcgag cgccttcatg atgctgcgat
     5161 agatcgccgc cggcatcgcc gacaccacca gcaccggcgt gccgcgctgc ttgttgatcg
     5221 tgttgatgat cgccacgccg gcgtggcgct cgcggccgag ctcgatgtcg agcacgacca
     5281 cgtcgtagtg ctcgcgcgcc agcgcgctct cggcgtcgtc gcggctgaac cactggtgca
     5341 cctcgatgcc ggggcgcgcc gcttcgatcc agccgcgcag ctggttgctg gtcggtacgt
     5401 cgtcctcgat caccgcgact ttgggcatgg cgcggattat cgagtcgggc ctcccggttc
     5461 cggaaccctt cgaggccgcg tacgctgcat cgaagagcgc cttaatggtc ttcggcgaca
     5521 gcttgcgcgt tgagctggac ggcgccgcga tcggtgtcac cattctcaat cccgcggtgg
     5581 tagacacaag attcttcgac cggcgtggcg tgcccta
//
LOCUS       DATMJB010000351         3118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_600215, whole genome shotgun sequence.
ACCESSION   DATMJB010000351 DATMJB010000000
VERSION     DATMJB010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600215"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="VNU48_07620"
     CDS             <1..423
                     /locus_tag="VNU48_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HWI11180.1"
                     /translation="PCDDVNRTFPAPSALAATKLERIAELGIILSRAGAIQAIAQAWS
                     EIAVLLAPGARPEPLIERLCALPGIGPWTAHYVAMRALGWPDAFPPNDVAVLKALARW
                     RATTTTTQREAEAHAQAWQPWRAYAVLRLWNSLEKTTP"
     gene            420..938
                     /locus_tag="VNU48_07625"
     CDS             420..938
                     /locus_tag="VNU48_07625"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HWI11181.1"
                     /translation="MNTTTDSIRAACTAQTTIATPLGPLLLARTARGLAGAWFEGQKH
                     HPGPLGAVRRPDDALLRAAADQLHAYFTGARDAFELPLDLHGTAFQRAVWQALLAIPG
                     GRTRSYGEIAGALGAASAVRAVGGAVGRNPLSVVVPCHRVVGSDGSMTGYAGGVERKR
                     ALLALERAPVAA"
     gene            964..1866
                     /locus_tag="VNU48_07630"
     CDS             964..1866
                     /locus_tag="VNU48_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWI11182.1"
                     /translation="MKNADVAELFALAALWGGSFLFMRMGAAEFGPVALAGVRVAGAA
                     LFLMPLLRLRGQVGVLRRHWRAIFVVGVTNSALPFLCFSYAALSITAGLAAIFNAASP
                     LFGALIAWLWLKDRLTPTRVLGLVIGFAGVLWLAWDTAGFKPGGSGWATLACVAATLL
                     YGGSASLTKRTLSGVAPLAVAAGSQLAAALVLAVPTLLWWPAQMPSARAWLTLALLAV
                     LCTGVAYVMYFRLIAHVGPANAIAVTFLVPAFAVLWGWLLLDERLSLAMLLGCAVILL
                     GTALATGFAKMPARTAKTKENSWT"
     gene            1857..2255
                     /locus_tag="VNU48_07635"
     CDS             1857..2255
                     /locus_tag="VNU48_07635"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWI11183.1"
                     /translation="MDLISIQQLLDPLFPGLMGVQLTELAADRVLATMVVRPDLCTAG
                     GILHGGAAMAFADTLGAVGTVVNLAAGKRTTTTDSSTKFIAGARVGTTVTGDCVALHR
                     GRTTMVWQTSIKNADGRLCAVVTQTQLVLD"
     gene            complement(2272..>3118)
                     /locus_tag="VNU48_07640"
     CDS             complement(2272..>3118)
                     /locus_tag="VNU48_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI11184.1"
                     /translation="DLYLALVERYRITHTQLVPTMFSRMLKLPEAARRRHDLSSLQIA
                     VHAAAPCPAQVKEQMIAWWGPIIHEYYGATEAMGFTACNSAEWLAHRGTVGKVLMGEL
                     HVLDEAMQPVPRGEPGELWFKTANPFVYFNDPQRTQATRSADGTMTTVGDVGYVDDDG
                     FLYLTDRSTFMIISGGVNIYPQECENLLITHPQVADAAVFGVPNDDLGEEVKAVVQPM
                     PGVVADDTFAQSLIAFCQEHLAHMKCPRSVDFTDALPRLPTGKLYKQPLRDRYWQGHG
                     RSRIV"
BASE COUNT          392 a         1117 c         1114 g          495 t
ORIGIN      
        1 ccctgcgacg acgtgaaccg cacgttcccg gcgccgtcgg cgctcgccgc gaccaagctg
       61 gagcgcatcg ccgagctcgg catcatcctc agccgcgccg gcgcgatcca ggcgatcgcg
      121 caagcctggt ccgagatcgc cgtgctgctc gcgccgggcg cgcggcccga gccgctgatc
      181 gagcgcctgt gtgcgctgcc cggcatcggc ccctggacgg cgcactacgt cgcgatgcgc
      241 gcgctcggct ggcccgatgc gttcccgccg aacgacgtcg cggtgctgaa ggcgctcgcg
      301 cgctggcgcg ccaccaccac gacgacccag cgcgaggccg aggcgcatgc acaggcctgg
      361 caaccgtggc gcgcgtatgc cgtgctgcgg ctttggaact ccttggagaa gacgacacca
      421 tgaacacgac caccgattcg atccgcgcgg cctgcaccgc acagaccacg atcgccacgc
      481 cgctcggccc gctgctgctg gcgcgcaccg cgcgcggcct ggcgggcgcg tggttcgagg
      541 ggcagaagca ccatcccggg ccactcggcg ccgtgcgccg gcccgacgat gcgctgctgc
      601 gcgcggccgc cgatcagctg cacgcgtact tcaccggcgc gcgcgacgcc ttcgagctgc
      661 cgctggacct gcacggcacc gcgttccagc gcgcggtgtg gcaggcattg ctcgcgattc
      721 ccggcggccg cacgcgcagc tacggcgaga tcgccggtgc gctcggcgcg gcttcggcgg
      781 tgcgcgcggt cggcggcgcg gtcgggcgca acccgctgtc ggtcgtcgtg ccctgtcacc
      841 gcgtcgtcgg cagcgacggc tcgatgaccg gctatgccgg cggcgtcgag cgcaagcgcg
      901 cgctgctcgc gctcgaacgc gcgccggtgg cggcatgagt gtttcgccgc gcagggccgc
      961 gcggtgaaga acgccgatgt cgccgagctg ttcgccctcg cggcgttatg gggcggctcg
     1021 tttctgttca tgcgcatggg tgcggccgag ttcgggccgg tcgcgctcgc gggcgtgcgc
     1081 gtcgcgggtg cggcgctgtt cctgatgccg ctgctgcggc tgcgcgggca ggtcggcgtg
     1141 ctgcgccgcc actggcgcgc gatcttcgtc gtcggcgtca caaactcggc actgccgttc
     1201 ctgtgcttca gctacgcggc gctgtcgatc acggccgggc tggcggcgat cttcaacgcg
     1261 gcctcgccgc tgttcggcgc gctgatcgcg tggctgtggc tgaaggaccg cctcacgccg
     1321 acgcgcgtgc tcggcctcgt gatcggcttc gccggcgtgc tctggctcgc ctgggacacg
     1381 gccggcttca agcccggtgg atcgggctgg gcgacgctcg cgtgcgtcgc cgcgacactg
     1441 ctctacggcg ggtcggcgag cctgacgaag cgcaccctga gcggcgtcgc accgctcgcg
     1501 gtggccgcgg gcagccagct cgcggccgcg ctcgtgctgg ccgtgcccac cttgctgtgg
     1561 tggccggcgc agatgccgtc ggcgcgcgcc tggctcacgc tcgcgctgct ggcggtgctg
     1621 tgcaccggcg tggcctacgt gatgtacttt cggctgatcg cccacgtcgg gccggccaac
     1681 gcgatcgcgg tgacctttct ggtgccggcg tttgcggtgc tgtggggctg gctgctgctc
     1741 gacgaacggc tgagcctggc gatgttgctc ggctgcgcag tgatcctgct cggcaccgcg
     1801 ctggccaccg gtttcgccaa aatgcccgca cgcacggcca agaccaagga gaactcatgg
     1861 acctgatctc gattcagcaa ctgctcgacc cgctcttccc cggcctgatg ggcgtgcagc
     1921 tcaccgagct cgcggccgat cgcgtgctcg ccacgatggt ggtccggccc gacctgtgca
     1981 ccgccggcgg catcctgcac ggcggcgccg ccatggcctt cgccgacacg ctcggcgcgg
     2041 tcgggacggt cgtgaatctc gcggccggca agcgcacgac gacgacggat tcgagcacca
     2101 agttcatcgc cggcgcacgc gtcggcacga ccgtcaccgg cgactgcgtg gcgctgcacc
     2161 gcggccgcac gacgatggtg tggcagacct cgatcaagaa cgccgacggc aggctctgcg
     2221 ccgtcgtcac gcagacccag ctggtgctcg actgaagcgc cggcgctgcg ctcagacgat
     2281 gcggctgcgg ccgtggccct gccagtagcg gtcgcgcagc ggctgcttgt agagcttgcc
     2341 ggtgggcagc cgcggcagcg cgtcggtgaa gtccacgctg cgcgggcact tcatgtgcgc
     2401 caggtgttcc tggcagaacg cgatcagcga ttgggcgaac gtgtcgtcgg cgaccacgcc
     2461 cggcatcggc tgcaccaccg ccttgacctc ctcgcccaga tcgtcgttgg gcacgccgaa
     2521 gaccgccgca tcggccacct gcgggtgcgt gatcagcagg ttctcgcact cctgcgggta
     2581 gatgttgacg ccgcccgaga tgatcatgaa ggtcgagcgg tcggtgagat agaggaagcc
     2641 gtcgtcgtcg acgtagccga cgtcgccaac ggtggtcatc gtgccatcgg ccgagcgcgt
     2701 ggcctgcgtg cgctgcggat cgttgaagta gacgaacggg ttcgcggtct tgaaccacag
     2761 ctcgccgggc tcgcccctgg gcacgggctg catcgcttcg tcgagcacgt gcagctcgcc
     2821 catcagcacc ttgccgaccg tgccgcggtg cgccagccat tcggcgctgt tgcaggcggt
     2881 gaagcccatc gcctcggtcg cgccgtagta ctcgtggatg atcggccccc accacgcgat
     2941 catctgctcc ttgacctgcg ccgggcacgg cgccgcggca tgcaccgcga tctgcagcga
     3001 cgacaggtcg tggcgccgcc gcgcggcctc gggcagcttg agcatgcggc tgaacatcgt
     3061 cggcacgagt tgcgtgtgcg tgatccggta ccgctcgacc agcgccaggt acagatcg
//
LOCUS       DATMJB010000352         5200 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_728814, whole genome shotgun sequence.
ACCESSION   DATMJB010000352 DATMJB010000000
VERSION     DATMJB010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728814"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VNU48_07645"
     CDS             <1..740
                     /locus_tag="VNU48_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HWI11185.1"
                     /translation="FEPLVEAARTPRGAFRVPLYGPPADLATRKPYWTRQQLDTLPAA
                     KASVRASVLAYVADPLDALILQIQGSGRLDIVETRGRHTLVRVAFAGHNDQPYTSIGR
                     WLIDQGALTPTQASWPGIKAWARQNPKRLNEMLWANPRVVFFREEPLPDPTLGPKGAQ
                     GVALTPGRSIAVDALAVPYGTPVWLDSTEPMSSAPLRRLVMAQDTGSAIAGAVRADYF
                     WGWGADAENQAGRMKQPLRLWALWPRS"
     gene            805..1290
                     /gene="msrB"
                     /locus_tag="VNU48_07650"
     CDS             805..1290
                     /gene="msrB"
                     /locus_tag="VNU48_07650"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HWI11186.1"
                     /translation="MTRRPLLALLCTLGLTARTGRAQTPATPSMPKIEPLRLTDAEWK
                     KRLAPAAYDVLRHEGTERPGSSPLNDEHRKGVFHCAGCELALFASDSKFDSGTGWPSF
                     FTSLPGAVVTKTDLKLIVPRTEYHCARCEGHQGHVFDDGPRPTGKRYCNNGVALKFVP
                     A"
     gene            complement(1310..2593)
                     /locus_tag="VNU48_07655"
     CDS             complement(1310..2593)
                     /locus_tag="VNU48_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HWI11187.1"
                     /translation="MHPNTHRTQPHRIPSFLRHVGPGVVTGAADDDPSGIATYTQAGA
                     QFGTGVLWTVFLTIPFMIGIQLVSARIGRQSGQGIAANLRAHTPRFFLFAIVGLLLVA
                     NTINIAADIAAMGEALQLVVGGGAHFYAVAFGALTLLLQVFVPYRRLAPILKWLTLSL
                     FVYVATAFTVHIPWSTVLHDLVLPRPIPDAAFWMMIVAILGTTISPYLFFWQAAQEVE
                     EMRLHGLKAGGESTRTLERAKRRMRLDTVIGMVFSNGIAFFVMLTGAIVLHGAGITDV
                     RSAAQAAQALRPLAGDFAFALFSLGIIATGLLAVPVLASSAAYAVAEAFAWPEGLERH
                     WREAKGFYAIIGIATIVGTGLDFTPIEPMKALYWSAVVNGVVAVPIMVAMMLLAGNSV
                     VMGGLPVRRKTRFLGWGAVLLMTAAVGMMLSDIVR"
     gene            complement(2722..4413)
                     /locus_tag="VNU48_07660"
     CDS             complement(2722..4413)
                     /locus_tag="VNU48_07660"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HWI11188.1"
                     /translation="MFDDYWEWVLREYPDFATYLGDHRYDDRLHDESAAAVGRRNVAT
                     AEFRERSARIDATQLSPADRTSLRVLQFRLERDLAITGLHGSLPFGVFDGWAPVTQMG
                     GIHLDLPQLANATRFESVRDYDAYLKRLNAVPDSVEQLIARMQIAIGAGWMPPRIAVS
                     GVPKQLEAQLGPDPRQSPEYQPFKSFPKDIATAEQERLAAAGRHVIQDRVIPAFQSLK
                     TFYETRYLPAAPERIGASNLPAGLPYYQAWLDWNTTTDMTPQQIRDLGLQEVARIGAQ
                     MDAIVATTGFKGSRAEFRRFIATDPQFFYTKPQDMLAGYRDIAKRADAELPKLFAELP
                     RQQYGIRAMRPEEGNNAEHYTGGAPDGSRAGWFEANVNDLKSRPKWTMETLLLHEAVP
                     GHHLQTARAQELGNLPRFRRHTWFTAYGEGWALYAESLGDEMGFYKDPYQKFGNLSFE
                     MLRACRLVVDTGLHAYGWTRQRAIDYMVDNTALTREEVTAEVDRYIVWPGQATAYKLG
                     ELRIKALRARAKAELGDRFDLRRFHNAVIDSGTVPLQVLDLQIDEWIASEKAALR"
     gene            4816..>5200
                     /locus_tag="VNU48_07665"
     CDS             4816..>5200
                     /locus_tag="VNU48_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter permease"
                     /protein_id="HWI11189.1"
                     /translation="MIDKRFFPRQVVESSGNRWDWALLPLVLALLVLLAYGGSQMARP
                     YQVGEALPLTLDPAYLPYYLLRTTLRMFLALGASLVFSCLFAAVAAKYRAAERVMVPL
                     LDILQSIPILGFLSITVTGFIALFPG"
BASE COUNT          808 a         1847 c         1772 g          773 t
ORIGIN      
        1 acttcgagcc gctggtcgag gccgcgcgca cgccgcgcgg cgcgttccgc gtgccgctgt
       61 acggcccgcc cgccgacctc gcgacccgca agccgtactg gacgcgccag cagctggaca
      121 cgctgcccgc cgcgaaggcg agcgtgcgcg ccagcgtgct cgcctacgtg gccgacccgc
      181 tggacgcgct gatcctgcag atccagggct cggggcgcct ggacatcgtc gagacgaggg
      241 gccggcacac gctggtgcgc gtcgcattcg ccggccacaa cgaccagcct tacacgtcga
      301 tcggccgctg gctgatcgac cagggcgcgc tgacgccgac gcaggcctcg tggccgggca
      361 tcaaggcctg ggcgcggcag aacccgaagc ggctgaacga gatgctgtgg gccaacccac
      421 gcgtcgtgtt cttccgcgaa gagccgctgc ccgacccgac gctcggcccg aagggcgcgc
      481 agggcgtggc gctaacgccg ggccgctcga tcgcggtcga tgcgctcgcc gttccgtacg
      541 gcacgccggt ctggctcgac agcaccgagc cgatgtcgag cgcgccgctg cgccgcctcg
      601 tgatggcgca ggacaccggc agcgcgatcg ccggcgcggt gcgcgccgac tacttctggg
      661 gctggggcgc cgacgccgag aaccaggccg gccggatgaa gcagccactg cgcctgtggg
      721 cactttggcc gcggagttga atcgcagcga aagattcgac gctgccgccc gacctatcgc
      781 cacacgaccc acggagatgc cgccatgacc cgccgccccc tgctcgcgct gctctgcacg
      841 ctcggcctga ccgccaggac cgggcgcgcc cagacaccgg ccaccccgag catgccgaag
      901 atcgagccgc tgcgcctgac cgacgccgaa tggaaaaagc gcctggcacc tgcggcctac
      961 gacgtgctgc gccacgaggg caccgagcgg cccggcagca gcccactgaa cgacgagcac
     1021 cgcaagggcg tgttccattg cgccggctgc gagctcgcgc tgttcgccag cgacagcaag
     1081 ttcgacagcg gcacgggctg gccgagcttc ttcacctcgc tgccgggcgc ggtcgtcacg
     1141 aagaccgacc tcaagctgat cgtgccgcgc accgagtacc actgcgcgcg ctgcgagggc
     1201 caccagggcc atgtgttcga cgacggcccg cgcccgaccg gcaagcgcta ctgcaacaac
     1261 ggcgtcgcgc tgaagttcgt gccggcctga ggcccccgag gccgcggcct cagcgcacga
     1321 tgtcggagag catcatgccc acggccgcgg tcatcagcag caccgcgccc cagccgagga
     1381 agcgcgtctt gcggcgcacc ggcagcccgc ccatgaccac cgaattgccg gccagcagca
     1441 tcatcgcgac catgatcggc accgcgacca cgccgttgac cacggcgctc cagtacagcg
     1501 ccttcatcgg ctcgatcggc gtgaagtcca gcccggtgcc gacgatcgtc gcgatgccga
     1561 tgatcgcgta gaagcccttc gcctcgcgcc agtggcgctc cagcccctcg ggccacgcga
     1621 aggcctcggc caccgcgtac gcggccgacg atgccagcac cggcaccgcg agcagcccgg
     1681 tggcgatgat gccgagcgag aacagcgcga acgcgaagtc gccggccagc gggcgcagcg
     1741 cctgcgcggc ctgcgccgcg ctgcgcacgt cggtgatgcc ggcgccgtgc aacacgatcg
     1801 cgccggtcag catcacgaag aacgcgatgc cgttcgagaa caccatgccg atcaccgtgt
     1861 ccagccgcat gcggcgcttc gcgcgctcga gcgtgcgggt gctttcgccg ccggccttca
     1921 ggccgtgcag gcgcatctcc tcgacctcct gggcggcctg ccagaagaac aggtagggcg
     1981 agatcgtggt gcccaggatc gccacgatca tcatccagaa cgccgcatcg gggatcggcc
     2041 gcggcagcac caggtcgtgc agcaccgtcg accacgggat gtgcaccgtg aacgcggtgg
     2101 cgacgtagac gaacaggctc agcgtcagcc acttcaggat cggcgcgagg cgccggtaag
     2161 gcacgaacac ctgcagcagc agcgtcagcg caccgaacgc gaccgcgtag aagtgcgcgc
     2221 cgccgccgac cacgagttgc agcgcctcgc ccatcgccgc gatgtcggcc gcgatgttga
     2281 tcgtgttggc caccagcagc agcccgacga ttgcgaacag gaagaaccgc ggcgtgtgcg
     2341 cccgcaggtt cgccgcgatg ccctggcccg actggcgccc gatgcgggcg ctgacgagct
     2401 ggatgccgat catgaacggg atcgtcagga acaccgtcca cagcacgccg gtgccgaact
     2461 gcgcgccggc ctgggtgtag gtcgcgatcc ccgacgggtc gtcgtcggcc gcgccggtca
     2521 ccaccccggg gccgacgtgc cgcaggaacg acgggatgcg gtgcggctgc gtgcgatggg
     2581 tgttcgggtg catgaccggg gcggcgccgc catcggcagc gggcgcaccc taccacgtcg
     2641 cagtggcggc cttcccggcc gggccgagtt tgcgccatgg cgcgccgcgg ccgcggactg
     2701 gcgaacgacc gccgagtgcc ttcagcgcag tgccgccttc tcgctggcga tccactcgtc
     2761 gatctgcaga tcgagcacct gcagcggcac cgtcccgctg tcgatcacgg cgttatggaa
     2821 gcggcgcagg tcgaagcgat cgcccaactc ggccttcgcc ctggcgcgca gcgccttgat
     2881 tctcaactcg ccgagcttgt acgctgtcgc ctgacccggc cagacgatgt agcgatcgac
     2941 ctcggccgtc acttcctcgc gtgtcagcgc agtgttgtca accatgtagt cgatggcccg
     3001 ctggcgcgtc cagccgtagg catgcagccc ggtgtcgacc accagccggc aagcgcgcag
     3061 catctcgaac gacaggttgc cgaacttctg gtacgggtct ttgtagaaac ccatctcgtc
     3121 gcccaggctc tcggcgtaca aggcccagcc ctcgccgtag gccgtgaacc aggtgtggcg
     3181 cctgaagcgc ggcaggtttc cgagctcctg cgcgcgcgcg gtctgcagat gatggccggg
     3241 caccgcctcg tgcagcaaca gcgtctccat ggtccatttc ggccgcgact tcaggtcgtt
     3301 cacattggcc tcgaaccagc cggcgcggct cccgtccggt gccccgccgg tgtagtgctc
     3361 ggcgttgttg ccttcctcgg ggcgcatcgc gcggatgcca tattgctggc gcggcagttc
     3421 ggcaaacagc ttcggcagct cggcatcggc gcgcttggca atgtcgcggt agccggccaa
     3481 catgtcttgc ggcttcgtgt aaaaaaactg cggatcggtc gcgatgaacc tccggaactc
     3541 ggcgcgcgaa cccttgaacc ctgtcgtggc gacgatcgca tccatctgcg cgccgatccg
     3601 ggccacctct tgcaggccca gatcgcggat ctgctgcggc gtcatgtcgg tggtcgtgtt
     3661 ccagtcgagc caggcttggt agtacggcag cccggccggc aggttcgacg caccgatgcg
     3721 ttcgggcgcc gctggcaggt agcgcgtctc gtagaaggtc ttcaaggatt ggaacgccgg
     3781 aatcacccgg tcctgaataa cgtgccggcc ggccgcggcg agccgctcct gctcggccgt
     3841 tgcgatgtcc ttggggaagg acttgaaggg ctggtactcg gggctctggc gtgggtcggg
     3901 accgagctgc gcttcgagtt gcttgggcac gcccgatact gcgatccggg gcggcatcca
     3961 gccggcgccg atcgcaatct gcatccgtgc gatcagttgc tccacactgt ccggtaccgc
     4021 gttgagccgc ttcaggtagg cgtcgtagtc gcgcaccgac tcgaaccggg tggcattggc
     4081 gagctgcggc agatcgagat ggatgccgcc catctgcgtg accggcgccc aaccgtcgaa
     4141 cacgccgaac ggcagcgatc cgtggagccc agtgatggcg aggtcgcgct ccaaccgaaa
     4201 ctgaagcacg cgcagcgaag tgcggtcggc gggcgagagt tgcgtggcgt cgatgcgagc
     4261 gctgcgctcg cggaactcgg cagtggcgac attgcgccgc ccgaccgcgg ccgccgattc
     4321 gtcgtgcagc ctgtcgtcgt agcgatggtc accaagatag gtggcgaaat cggggtactc
     4381 gcgcagcacc cattcccagt agtcatcgaa caaggcctgg gcctgagcgg tcgcagcggg
     4441 ctgcgcaggc tgtgccgcga cccagcttgc gaacaacagc gcgactgaga acgcgactgc
     4501 gtccaaggcc tggcggcatc gcatggcgga cctccctcga cgggcaccgc atggggcgac
     4561 gcggcgcgag gtggcgagtc taggcgccag tcccgtcggt gtcatccgca gtgtgcagga
     4621 cccccggcca tgcaggacag ggcgcgcgag gtgacgacat cgacgtgggc tcccggactt
     4681 aagcgtcggc cgcggcgtcg atgatcccat gcaaagctgt gcaaaccggc ttgcaggccg
     4741 cgcgggcctg tcacgcggcg cggtgacaat cgcccacccc gccggggccc gtgtcacgcc
     4801 cgtccgccct ctccgatgat cgacaagcgc ttcttccccc gccaggtggt cgagtccagc
     4861 ggcaaccgct gggactgggc cctgctgccg ctggtgctgg cgctgctggt gctgctcgcg
     4921 tacggcggct cgcagatggc gcggccgtac caggtgggcg aggcgctgcc gctgacgctg
     4981 gacccggcct acctgcccta ctacctgctg cggaccacgc tgcgcatgtt cctcgcgctc
     5041 ggcgcgtcgc tggtcttcag ctgcctcttc gcggcggtcg cggcgaagta ccgcgcggcc
     5101 gagcgcgtga tggtgccgct gctcgacatc ctgcagtcga ttccgatcct gggcttcctg
     5161 tcgatcacgg tgaccggctt cattgccctg ttccccggca
//
LOCUS       DATMJB010000353         2164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_805132, whole genome shotgun sequence.
ACCESSION   DATMJB010000353 DATMJB010000000
VERSION     DATMJB010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805132"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VNU48_07670"
     CDS             complement(<1..415)
                     /locus_tag="VNU48_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11190.1"
                     /translation="MRYRIALCGFSEFEYRAMHFSFQHPPEFQESSYDVVDALSEADF
                     AVVDADSQPAVKGVIRSGRVAQAVFVGATAPDGAASHLQRPIDPRRILRTLERLTTRH
                     PGGAPAFKLPVPEVSELPTLEDAVTMAAEPSEPASP"
     gene            638..1042
                     /locus_tag="VNU48_07675"
     CDS             638..1042
                     /locus_tag="VNU48_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HWI11191.1"
                     /translation="MPHLVILYSGNLDAQADMGALCRGLADTMLAQLDEAGAPVFPPG
                     GTRVLAYPAPRFAIADGQHDYAFAYLNLRMGRGRSEASQQRIGDALLATAKAFFEPIQ
                     VRRLVGVTIQIDIGAEVFDAKIGNIHPLFAKD"
     gene            complement(1083..>2164)
                     /locus_tag="VNU48_07680"
     CDS             complement(1083..>2164)
                     /locus_tag="VNU48_07680"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HWI11192.1"
                     /translation="GTGSFGSAPVSGFGSIFVGGVEFDDSRAAVFDDDGVALRAGGTP
                     MRLGMITEVEGGAVSAGPAGPTAVASAVHIARLAVGPAANVDIAGHTLTVLGQTLQVN
                     GSTVFDPALHGGLAGLHAGDMVSVHALADAAGRPVATRIEPAASGEAWRLRGFVSATN
                     AQAKRFTIGTATLDYGTASNIPTDLADQQFVQVRLAGAGAGGVLQVSSFLPASAAPPD
                     SASVVTEGVVAALNPGAAFRIGALAVDITGAVVLPGPVALVAGAQAQVEGRLQAGMLV
                     ADKVTVLTTQAADQRSYQLAGTVSALNAANLTFVVRNVGVDYSTAHFSNGSAVTLADP
                     NVSVHLQGSLSADGTMVQAKQISFP"
BASE COUNT          340 a          795 c          735 g          294 t
ORIGIN      
        1 ctggagacgc aggctccgaa ggctcggccg ccatcgtgac ggcgtcctcg agcgtcggca
       61 actcgctcac ctcgggcacc ggcaacttga acgccggcgc gccccccggg tgacgcgtgg
      121 tcaaccgctc gagcgtgcgc aggatgcgcc gggggtcgat cgggcgctgc aggtgcgagg
      181 cggcgccgtc cggcgccgtg gcgccgacga agaccgcctg cgcgacccgc cccgaccgga
      241 tcacgccctt gaccgcgggc tgcgagtcgg cgtcgaccac ggcgaagtcg gcctccgaca
      301 gggcgtcgac cacgtcgtag ctcgactcct ggaactcggg cggatgctgg aacgagaagt
      361 gcatcgcccg gtactcgaac tcgctgaatc cgcagagtgc aatccggtag cgcatatgct
      421 ggcaaggcct cccgtggcgc gccggtctcg gggtcggcac agcgcgaagg atacgcaatt
      481 cgatcgcctg ccgtcacccc caacgcaggc atgctcgccg cgagcgcgcg atcgagatca
      541 tcacgccata ctccggcagt tcgggaaccg gccagatcaa cgccatcttc ccggtcatgc
      601 cgctgcccgc ggcctgatcc tgccccaagg aagacccatg ccccacctcg tgatcctgta
      661 cagcggcaac ctcgacgcgc aggccgacat gggggcgctg tgccgcggcc tggccgacac
      721 gatgctcgcg cagctcgacg aggccggcgc gccggtgttc ccgcccggcg gcacgcgggt
      781 gctggcctac ccggcgccgc gcttcgcgat cgccgacggc cagcacgact acgcgtttgc
      841 ctacctgaac ctgcgcatgg ggcgcgggcg cagcgaggcg agccagcagc gcatcggcga
      901 cgcgctgctg gccaccgcga aggccttctt cgagccgatc caggtgcgcc ggctggtcgg
      961 cgtgacgatc cagatcgaca tcggcgccga ggtcttcgac gccaagatcg gcaacatcca
     1021 cccgctgttc gcgaaggact gacgcgtgct cgtgaagcgc gatcggcgac accgtcaacg
     1081 catcaaggaa acgagatctg cttcgcctga accatcgtgc cgtcggccga cagcgaccct
     1141 tgaaggtgta cagagacgtt cggatcggca agcgtcaccg cactgccgtt ggaaaagtgc
     1201 gccgtgctgt agtcgacgcc gacgttgcgg accacgaagg tcagattggc ggcgttcaac
     1261 gcgctcaccg tcccggccag ctggtagctg cgctgatcgg cggcctgggt cgtcagcacc
     1321 gtcaccttgt cggcgacgag catgccggcc tgcagtcgtc cctcgacctg tgcctgcgca
     1381 ccggccacga gtgcgaccgg cccgggaagg acaacggcgc ccgtgatgtc gacggccagc
     1441 gcgccgatgc gaaatgcggc tcccgggttg agcgccgcga ccacgccttc ggtcacgacg
     1501 ctcgccgagt cgggcggtgc ggccgatgcc ggcaggaaac tgctcacctg cagcacaccg
     1561 cccgcgcccg cgccggccag cctgacctgc acgaactgct ggtcggccag gtcggtcggg
     1621 atattggatg ccgtgccgta gtccagcgtg gccgtgccga tcgtgaagcg cttcgcctgc
     1681 gcattcgtcg ccgagacgaa accgcgcagg cgccatgctt cccccgacgc ggccggctcg
     1741 atgcgggtgg cgaccgggcg ccccgcggcg tcggccagcg cgtgcaccga caccatgtcg
     1801 ccggcgtgaa ggcccgccag accgccatgc agcgccggat cgaacacggt tgagccgttg
     1861 acctggagcg tctggcccag cacggtgagc gtgtggccgg cgatgtcgac gttcgccgcc
     1921 ggcccgacgg ccaggcgcgc gatgtgcacc gccgacgcga cggccgtcgg ccccgcgggg
     1981 ccggcgctca ccgcgccgcc ctcgacttcc gtgatcatgc ccagccgcat cggcgtgccg
     2041 ccggcgcgca gcgcgacacc gtcatcgtcg aacacggcgg ccctggagtc gtcgaactcg
     2101 acgcccccga cgaatatcga tccgaaaccg ctgaccggcg ccgagccgaa cgagcccgtg
     2161 cccc
//
LOCUS       DATMJB010000354         4170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_248425, whole genome shotgun sequence.
ACCESSION   DATMJB010000354 DATMJB010000000
VERSION     DATMJB010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248425"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /gene="tolB"
                     /locus_tag="VNU48_07685"
     CDS             <1..993
                     /gene="tolB"
                     /locus_tag="VNU48_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HWI11193.1"
                     /translation="ALVAGSVTRLADGRFDVRFKLWDVVKGNVLGGQSTAVDAADLRL
                     AAHRIADFIYEKLTGDKGVFSTRIAYVTKGGNRYTLRVADADGEGGQVALNSPEPIIS
                     PAWSPDGRQLAYVSFEKQKAVVYTQDTSSGARREVANFRGSNSAPAWSPDGQTLALTL
                     SREHGSQLYLMDRSGGNVRRLTTSNAIDTEPAWSPDGRSVYFVSDRGGGPQIYRTPAA
                     GGSVERVTFGGNYNISPAISPDGRTLAYISRQGGAFRLYTLDLSGGAQPVALTDTSDD
                     ESPSFAPNGRLIIYASRAQGRDVLMTTTLDGKIKARLVSTTADVREPVWGPFGR"
     gene            1039..1605
                     /gene="pal"
                     /locus_tag="VNU48_07690"
     CDS             1039..1605
                     /gene="pal"
                     /locus_tag="VNU48_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HWI11194.1"
                     /translation="MNQNSTRFAPTTRVLSLSALLAAAALLAGCSSSVPLNDAGGAPV
                     ESRTIGAGSGAGTGGASESRVTPVDLTKGANDANGGAQNIVYFDFDSFVVKDEYRPVI
                     DRFAKALAADRKKHLQVEGHTDERGGREYNLALGQKRAEAVAKSLALLGAADSQVEAV
                     SFGKERPAVQGSDEAAYAKNRRAELNYR"
     gene            1614..2390
                     /gene="ybgF"
                     /locus_tag="VNU48_07695"
     CDS             1614..2390
                     /gene="ybgF"
                     /locus_tag="VNU48_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HWI11195.1"
                     /translation="MRTTTLRAGVAGLCLGAALFAPAARAGLFDDDEARRAILDIRQK
                     IEQNAERQRTGQQQLTEQIDQLKRSLLDLNNQLEQMRGDNASLRGQNEELTRIVSEIQ
                     RKQKDLQAGVDDRIRKIEPQKVSVDGKEFNADPEEKRQYDDAIELLRKSDFAGTANAL
                     SAFRRRWPASGYNESALFWLGNAQYGVRQYKEAITSFRTLVAGSPDHPKAPEALLAIA
                     NCQAELKDGKGARATIGELVKTYPKSEAAQAGRDRLASLK"
     gene            2435..3556
                     /locus_tag="VNU48_07700"
     CDS             2435..3556
                     /locus_tag="VNU48_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HWI11196.1"
                     /translation="MQEVDVKALATGVLWASFGLSALFGAIAQRTHFCTMGAVSDIVT
                     IGDWTRMRMWVMAMGVAMIGFNLMVAAGWLDAGKSLYGGPKFIWLSALVGGTLFGFGM
                     VLASGCGSKTLVRIGAGNLKSLVVFVVLGVSAFATLKGITAVARVATVDTVAITFGSG
                     QDLPSLVAAGTGAAKAMLAAVLGLAIGGALIAWSLAKAEGRSADNLLAGLGVGAIIAG
                     VWWVSGRYGHVAEDPNTLQEAFVATNSQRMESLSFVAPVAYTLDWLMFFSDKSKALTL
                     GIVSVVGVVVGSGAYALATDRFRWEGFRDAGDTANHLIGALLMGVGGVTALGCTIGQG
                     LSGVSTLSLTSFVAVAAIIGGAVAALRYQSWRVERGMTP"
     gene            3553..>4170
                     /locus_tag="VNU48_07705"
     CDS             3553..>4170
                     /locus_tag="VNU48_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HWI11197.1"
                     /translation="MNQRADVLPLALDADLERRFGGLRRLYGDAGYARIRAARVAIVG
                     VGGVGSWCAEALARSGVAALTLIDLDHVAESNINRQVQALGTTLGQAKVQALRERIAD
                     IHPGCDVRCVEEFVDETNWPTLLAQPVDVVIDACDQVRAKAAIAAWALRSRSALVVAG
                     AAGGKRRPQEVEVADLGLVTHDPLLAALRQRLRKEHGAARTGKIGV"
BASE COUNT          648 a         1429 c         1521 g          572 t
ORIGIN      
        1 gcgctggtcg ccggctcggt gacccggctc gccgacggcc gcttcgacgt gcgcttcaag
       61 ctctgggacg tggtcaaggg caacgtgctc ggcggccaga gcaccgcggt cgacgccgcc
      121 gacctgcgcc tggcggcgca ccgcatcgcc gacttcatct acgagaagct gaccggcgac
      181 aagggcgtgt tctcgacccg catcgcctac gtgaccaagg gcggcaaccg ctacacgctg
      241 cgcgtggccg acgccgacgg cgagggcggg caggtcgcgt tgaacagccc cgagcccatc
      301 atctcgccgg cctggtcacc cgacggcagg cagctcgcct acgtgtcgtt cgagaagcag
      361 aaggccgtgg tctacacgca ggacacgtcg agcggcgcgc gccgcgaagt ggcgaacttc
      421 cgcggctcga acagcgcgcc ggcctggtca ccggacggcc agacgctggc actcacgctg
      481 tcgcgcgaac acggctcgca gctctacctg atggaccgca gcggcggcaa cgtgcggcgc
      541 ctgacgacca gcaacgcgat cgacaccgag ccggcctggt cgcccgacgg ccgcagcgtc
      601 tacttcgtca gcgatcgtgg cggcggcccg cagatctacc gcacgccggc ggccggcggg
      661 agtgtcgagc gcgtcacctt cggcggcaac tacaacatca gcccggcgat cagccccgac
      721 ggccgcacgc tcgcgtacat ctcgcgccag ggcggggcgt tccgcctgta cacgctcgac
      781 ctgagcggcg gcgcgcagcc ggtcgcgctg accgacacca gcgacgacga aagcccgagc
      841 ttcgcgccga acggccggct gatcatctac gcctcgcgcg cccagggccg cgacgtgttg
      901 atgacgacca cgctggatgg caagatcaag gcacggctgg tgtcgaccac cgccgatgtg
      961 cgcgagccgg tgtggggccc gttcggccgc taggcgcgcc gcgcggcccg gtgaattttc
     1021 tttgaagagg atgcaacgat gaaccagaac tcaacccgct tcgcgcccac gactcgcgtg
     1081 ctgtccttgt cggcgctgct ggccgcggcg gcgctgctgg cgggctgttc ctccagcgtg
     1141 ccactgaacg acgccggcgg cgccccggtc gagagccgca cgatcggcgc cggcagcggt
     1201 gcgggcacgg gcggcgcgag cgagagccgt gtcacgccgg tcgacctgac gaagggcgcg
     1261 aacgatgcca acggcggcgc gcagaacatc gtctacttcg acttcgacag cttcgtcgtg
     1321 aaggacgagt accggccggt catcgaccgc ttcgcgaagg cgctggcggc cgatcgcaag
     1381 aagcacctgc aggtcgaggg ccacaccgac gagcgcggcg ggcgcgaata caacctcgcg
     1441 ctcggccaga agcgcgccga ggcggtcgcg aagtcgctcg cgctgctcgg cgcggccgat
     1501 tcgcaggtcg aggccgtgag cttcggcaag gagcgcccgg cggtgcaggg cagcgacgag
     1561 gcggcctacg cgaagaaccg ccgcgccgag ctgaactacc gatgagcgcg acgatgcgaa
     1621 cgacgactct tcgcgcaggc gtcgccgggc tctgcctcgg cgcggcgctg ttcgccccgg
     1681 ccgcgcgcgc cggcctgttc gacgacgacg aggcgcgccg cgcgatcctc gacatccgcc
     1741 agaagatcga gcagaacgcc gagcgccagc gcaccggcca gcagcagctg acggagcaga
     1801 tcgaccagct caagcgcagc ctgctcgacc tgaacaacca gctcgagcag atgcgcggcg
     1861 acaacgcgag cctgcgcggg cagaacgagg agctgacgcg catcgtctcg gagatccagc
     1921 gcaagcagaa ggacctgcag gcgggtgtcg acgaccgcat ccgcaagatc gagccgcaga
     1981 aggtctcggt cgacggcaag gagttcaacg ccgaccccga agagaagcgc cagtacgacg
     2041 acgcgatcga gctgctgcgc aagagcgatt tcgccggcac tgcgaacgcg ctcagcgcct
     2101 tccgcaggcg ctggccggcc agcggttaca acgaatcggc gctgttctgg ctcggcaacg
     2161 cgcagtacgg cgtgcgccag tacaaggagg cgatcacctc gtttcgcacg ctggtggccg
     2221 gctcccccga ccaccccaag gcgcccgaag cgctgctcgc gatcgcgaac tgccaggccg
     2281 aactgaagga cggcaagggc gcgcgcgcga ccatcggcga actggtcaag acctacccca
     2341 agtccgaggc cgcacaggcc gggcgcgacc ggctcgcgtc gctgaagtga gcgggcggcc
     2401 gcggccgccc gaaggccgca cctaaaatgc ggcgatgcaa gaagtcgatg tgaaggctct
     2461 cgcgaccggg gtgctgtggg cctcgttcgg cctgtcggcg ctgttcggcg cgatcgcgca
     2521 gcgcacccat ttctgcacga tgggcgcggt ctccgacatc gtcaccatcg gcgactggac
     2581 gcgcatgcgc atgtgggtca tggcgatggg cgtggcgatg atcggcttca acctgatggt
     2641 ggccgcgggc tggctcgacg ccggcaagag cctctacggc gggccgaagt tcatctggct
     2701 ctcggcgctg gtgggcggca cgctgttcgg cttcggcatg gtgctggcgt cgggctgcgg
     2761 cagcaagacg ctggtgcgca tcggcgcggg caacctgaag tcgctggtcg tgttcgtggt
     2821 gctcggggtg tcggcgttcg cgacgctgaa gggcatcacc gcggtcgccc gcgtcgcgac
     2881 cgtcgacacg gtggcgatca ccttcggcag cgggcaggac ctgccgtcgc tcgtcgcggc
     2941 cggcaccggc gcggcgaagg cgatgctcgc ggcggtgctc ggcctggcga tcggcggcgc
     3001 actgatcgca tggtcgctcg ccaaggccga aggccgctcg gccgacaacc tgctggcggg
     3061 cctgggcgtg ggcgcgatca tcgccggcgt ctggtgggtg tcgggccgct acggccacgt
     3121 cgccgaagac ccgaacacgc tgcaggaggc gttcgtcgcc accaactcgc agcgcatgga
     3181 gtcgctcagc ttcgtcgcgc cggtggcgta cacgctggac tggctgatgt tcttcagcga
     3241 caagtcgaag gcgctgacgc tcggcatcgt cagtgtcgtc ggcgtcgtcg tcggctcggg
     3301 cgcgtatgcg ctggcgaccg atcgctttcg ctgggagggc ttccgcgacg ccggcgacac
     3361 cgccaaccac ctgatcggtg cgctgctgat gggcgtgggc ggcgtgacgg cgctgggctg
     3421 cacgatcggg cagggcctgt cgggcgtgtc gacgctgagc ctgacgagct tcgtcgcggt
     3481 ggcggcgatc atcggcggtg cggttgcggc actgcgctac cagagctggc gcgtcgagcg
     3541 cgggatgacg ccatgaatca gcgcgccgat gtgttgccac ttgcgctcga cgccgacctc
     3601 gagcgtcgct tcggcgggtt gagacgtctc tacggcgacg cgggctacgc gcgcatccgc
     3661 gccgcgcggg tcgcgatcgt cggcgtcggc ggcgtcggct cgtggtgcgc cgaggcgctg
     3721 gcgcgcagcg gcgtcgccgc gctgaccttg atcgatctcg accacgtcgc cgaatcgaac
     3781 atcaaccgcc aggtgcaggc gctcggcacc acgctcggcc aggccaaggt gcaggcgctg
     3841 cgcgagcgca tcgcggacat ccaccccggc tgcgacgtgc gctgtgtcga agagttcgtc
     3901 gacgagacga actggccgac gctgctggcg cagccggtcg acgtggtgat cgacgcctgc
     3961 gatcaggtgc gcgcgaaggc ggcgatcgcg gcgtgggcac tgcgcagccg cagtgcgctc
     4021 gtggtcgccg gcgctgcggg cggcaagcgg cgcccgcagg aggtcgaggt cgcggatctg
     4081 gggctcgtca cgcatgaccc gctgctggcg gcgctgcgcc agcgcctgcg caaggagcac
     4141 ggtgcggcgc gcaccggcaa gatcggcgtg
//
LOCUS       DATMJB010000355         2590 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_104941, whole genome shotgun sequence.
ACCESSION   DATMJB010000355 DATMJB010000000
VERSION     DATMJB010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104941"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2035
                     /locus_tag="VNU48_07710"
     CDS             <1..2035
                     /locus_tag="VNU48_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HWI11198.1"
                     /translation="AGLRKLFLQFSFPGGIPSHASPETPGSIHEGGELGYSLSHSFGA
                     AFDNPALIVACVVGDGEAETGPLATAWHSNKFLDPVHDGAVLPILHLNGYKIANPTIL
                     ARIPHDELEQLLSGYGWQPIFVEGHEPLPMHAAMASALDAAVMRIQAIQAQARDAGVT
                     SRPRWPMIVLRSPKGWTGPKRVDGQQIEGAFRSHQVPLSDPAAHPEHLALLEDWLRSY
                     RPQELFDAQGRLMPELADLAPRGSRRMGANPHANGGLLLRDLHLPDYQRYAVDVTSPG
                     VRGVGDTRVLGPFLRDVATLNDAQRNFRVFGPDETISNGLAALFEATDRPWQAATEPN
                     DEFLAPSGRVVEMLSEHQCEGWLEGYLLTGRHGLFNCYEAFIHIVDSMFNQHAKWLKV
                     SAQLPWRRKIASLNYLLASHVWRQDHNGFTHQDPGFIDHVVNKKAEVVRVYLPPDANC
                     LLSVMDHCLRSRHCVNVVVAGKHPAPQWLTMDDAVKHCAAGVGIWPWASNDQGGEPDV
                     VMACCGDVPTLETLAAVTILREQLPELKLRVVNVVDLMKLQPSSEHPHGLSDAAFDAL
                     FTTDKPVIFAYHAYPTLIHRLTYRRTNHANIHVRGYKEEGTITTPFDMTVLNDLDRFH
                     LVMDVIHRLPQTGARGIEVRRRLEDKLVEHRRYINRHGQDMPEIRDWTWSASA"
     gene            complement(2058..2303)
                     /locus_tag="VNU48_07715"
     CDS             complement(2058..2303)
                     /locus_tag="VNU48_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11199.1"
                     /translation="MAGMEKPRTSHPWIARYCIRTLQLLPLLPAPVAIRWAVHGYPYC
                     GDEEPERAAEMFVDARNAHDTGRLPPARRDTDQRSRR"
BASE COUNT          424 a          929 c          855 g          382 t
ORIGIN      
        1 agcggggctg cgcaagctgt tcctccagtt ctcgtttccg ggcggcatcc cgagccacgc
       61 gtcgcccgaa acgccgggct cgatccacga aggcggcgaa ctcggctatt cgctcagcca
      121 ctcgttcggc gcggcgttcg acaaccccgc gttgatcgtc gcctgcgtgg tcggcgacgg
      181 cgaggccgag accgggccgc tggccacggc ctggcactcg aacaagttcc tcgaccctgt
      241 gcacgacggc gcggtgctgc cgatcctgca cctgaacggc tacaagatcg ccaacccgac
      301 gatcctcgcg cgcatcccgc acgacgagct ggagcagctg ctgagcggct acggctggca
      361 gccgatcttc gtcgagggcc acgagccctt gccgatgcac gcggcgatgg cgagcgcgct
      421 cgatgcggcg gtgatgcgca tccaggcgat ccaggcgcag gcccgcgatg cgggcgtcac
      481 gtcgcgcccg cgctggccga tgatcgtgct gcggtccccg aagggttgga ccgggccgaa
      541 gagggtggat ggccagcaga tcgagggcgc attccgctcg caccaggtgc cgctgtcgga
      601 cccggccgcg catcccgagc acctcgcgct gctcgaggac tggctgcgca gctaccggcc
      661 gcaggagctg ttcgacgcgc aggggcggct catgcccgag ctcgccgacc tcgcgccgcg
      721 cgggtcacgc cgcatgggcg ccaatccgca tgccaacggc ggtctgctgc tgcgcgacct
      781 gcacctgccc gactaccaac gctacgcggt cgacgtgacc agccccggcg tgcgcggtgt
      841 cggcgacacg cgtgtgctcg ggccgttcct gcgcgacgtg gcgacgctga acgacgcgca
      901 acgcaacttc cgtgtcttcg ggcctgacga gaccatctcc aacggcctgg ccgcgctgtt
      961 cgaggcgacc gaccgcccat ggcaggccgc gaccgaaccg aacgacgagt tcctcgcgcc
     1021 cagcgggcgc gtcgtcgaga tgctcagcga gcaccagtgc gaaggctggc tcgagggcta
     1081 tctgctgaca gggcgccacg gcctgttcaa ctgctacgag gccttcatcc acatcgtcga
     1141 ctcgatgttc aaccagcatg ccaagtggct gaaggtcagc gcgcaacttc cgtggcggcg
     1201 caagatcgcc tcgctgaact acctgctcgc ttcacacgtc tggcgtcagg accacaacgg
     1261 cttcacgcac caggacccgg gcttcatcga tcacgtcgtc aacaagaagg ccgaggtcgt
     1321 gcgcgtgtac ctgccgcccg atgcgaactg cctgctgtcg gtgatggacc actgcctgcg
     1381 cagccgccac tgcgtgaacg tcgtggtcgc cggcaagcac ccggcaccgc agtggctgac
     1441 gatggacgat gccgtcaagc actgcgccgc gggcgtcggc atctggccct gggcgagcaa
     1501 cgaccagggc ggcgagcccg acgtggtgat ggcctgctgc ggcgacgtgc ccacgctcga
     1561 gacgctcgcc gccgtgacca tcctgcgcga gcagctgccc gagctgaagc tgcgcgtcgt
     1621 caacgtcgtc gacctgatga agctgcagcc gtcgtcagag catccgcacg ggctgtcgga
     1681 cgcggccttc gacgccctct tcacgacgga caagccggtc atcttcgcgt accacgcgta
     1741 cccgacgctg atccaccggc tgacctaccg gcgcaccaac cacgccaaca tccatgtccg
     1801 cggctacaag gaggagggca cgatcaccac gcccttcgac atgacggtgc tgaacgacct
     1861 ggatcgcttc cacctcgtga tggacgtgat ccatcgcctg ccgcagaccg gcgcgcgcgg
     1921 catcgaggtc aggcgacggc tcgaggacaa gctggtcgag catcggcgct acatcaaccg
     1981 ccacggccag gacatgccgg agatccgcga ctggacctgg agcgcctcag cgtgaacgaa
     2041 cctgcgctcg ccccggatca gcggcgcgac cgctggtcgg tgtcgcgccg ggcgggcggc
     2101 agccggcccg tgtcgtgcgc gttgcgcgcg tcgacgaaca tctcggccgc gcgttcgggc
     2161 tcctcgtcgc cgcaataggg atagccgtgc accgcccacc ggatcgcgac cggtgcgggc
     2221 agcaagggca acaattgcag cgtgcggatg cagtagcgcg cgatccaggg gtgcgaagtg
     2281 cggggctttt ccatgccggc cattcttcga tgcctgcacc cggcaggttt gtaggacgtt
     2341 gccgcatcga cgggtcggac ggcggacggc ccggccgcgc ggggccgcgt gactagaatc
     2401 gccactcgcc gtacctcccg ccatccattc aagacttccg gagcccgcga atgaatcgcc
     2461 tgccgactgc tctgattgcc ctcgcctttg cgagcctcgc ccaagcggcc gaacccacca
     2521 cggccgaacc ggcgcagtcc accgcgtcgt cggtcgcgac caagaccggc aaggccatca
     2581 agaagggcgc
//
LOCUS       DATMJB010000356         2941 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_865979, whole genome shotgun sequence.
ACCESSION   DATMJB010000356 DATMJB010000000
VERSION     DATMJB010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865979"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VNU48_07720"
     CDS             complement(<1..559)
                     /locus_tag="VNU48_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HWI11200.1"
                     /translation="MAEYHEAPANATAAQKLLNWVDNRFPLSKLYNEHMGEYYAPKNF
                     NFWYIFGSLAMLVLVLQIVTGIFLTMNYKPDAGMAFASVEYIMRDVPGGWIIRYMHST
                     GASAFFIVVYLHMFRGLLYGSYRKPRELVWIFGCGIFLCLMAEAFMGYLLPWGQMSYW
                     GAQVIVNLFAAVPFIGPDLALLIRGD"
     gene            complement(604..1206)
                     /gene="petA"
                     /locus_tag="VNU48_07725"
     CDS             complement(604..1206)
                     /gene="petA"
                     /locus_tag="VNU48_07725"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HWI11201.1"
                     /translation="MSDQQIDSSKRTWIIASSCAGAVGTGFAAVPFVASFQPSERARA
                     AGAAVEADISGLKPGEKMTVEWRGKPVWIVRRTPEQLAELSKVDKELADPESKRTGFS
                     TTPEYARNDWRSIKKEYLVVVGICSHLGCSPSDKFVPGAQPSLPNDWPGGFLCPCHGS
                     TFDMAGRVFKDKPAPDNLEVPPYMYLSDTRLMIGEDNKKA"
     gene            1360..1788
                     /gene="mscL"
                     /locus_tag="VNU48_07730"
     CDS             1360..1788
                     /gene="mscL"
                     /locus_tag="VNU48_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWI11202.1"
                     /translation="MSFSSEFKEFAIKGNVMDLAVGVIIGAAFGKIVDSIVKDLIMPV
                     VGRVFGGLDFSNYFVMLAAPPATYNGPMSYEALTKAGVPLFAYGNFITVAINFIILAL
                     IIFVMIKQVNRLKKASPPPAPPATPEGIVLLREIRDSLKK"
     gene            1945..>2941
                     /locus_tag="VNU48_07735"
     CDS             1945..>2941
                     /locus_tag="VNU48_07735"
                     /inference="COORDINATES: protein motif:HMM:NF019410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HWI11203.1"
                     /translation="MIPIEPRLRAALDAATSQIRATLTEVASTLSDTLGTVPQAHPGE
                     RELLNSARFELRRNLASMHLAFDQELNKRVTEDLTPRDEPKRTRLAATNWQSLTLVDD
                     SEVEGQMVANRIGQQIWHACESELRDLASYMGSLLKTDRSDQDRNPLRADVVGASLYR
                     AVEAVSNQAETRKVLAREFGNAMAKAMPACYAEIVRGLQARGIEPVSLAVRQVDGPGN
                     QLPGLNSGYASQRSDLDPLSSLGSGSAMSGYEQRSGRGGSSGLHSGPGASTHGGYSRS
                     GPGSQHASGPVLADAQLMALLRHLTAVASRPGELDAAGLGSRYGGAGAAAAGPIAA"
BASE COUNT          492 a          974 c          990 g          485 t
ORIGIN      
        1 agtcgccgcg aatcaggagc gccaggtccg ggccgatgaa cggcacggcg gcgaacaggt
       61 tcacgatcac ttgcgcgccc cagtagctca tctggcccca cggcagcaga tagcccatga
      121 acgcctcggc catcaggcac aggaagatgc cgcagccgaa gatccagacc agctcgcgcg
      181 gcttgcggta gctgccgtac agcagcccgc ggaacatgtg caggtacacg acgatgaaga
      241 acgcgctcgc gccggtcgag tgcatgtagc ggatgatcca gccgcccggc acgtcgcgca
      301 tgatgtactc gaccgacgcg aaggccatgc ccgcgtcggg cttgtagttc atcgtcagga
      361 agatgccggt gacgatctgc agcaccagca ccagcatcgc cagcgagccg aagatgtacc
      421 agaagttgaa attcttcggg gcgtagtact cgcccatgtg ctcgttgtag agcttggaca
      481 gcgggaaccg gttgtcgacc cagttcagca gcttctgggc cgccgtcgcg ttggcggggg
      541 cttcgtggta ttcggccatc ttggtggtac tcctggggtg ccgtcttggt gcctgaaccg
      601 gggtcaggcc ttcttgttgt cttcgccgat catcagccgg gtgtccgaca ggtacatgta
      661 cggcggcact tcgaggttgt cgggcgcggg cttgtccttg aacacgcggc cggccatgtc
      721 gaaggtcgag ccgtgacatg ggcacaggaa gccgcccggc cagtcgttcg gcagcgaggg
      781 ctgcgcgccg ggcacgaact tgtcgctcgg cgagcagccc aggtgcgagc agatgccgac
      841 gacgaccagg tattccttct tgatcgagcg ccagtcgttg cgcgcgtatt cgggcgtggt
      901 cgagaagccc gtgcgcttgg attcggggtc ggcgagttcc ttgtcgacct tggacagctc
      961 ggcgagctgc tcgggcgtgc gccgcacgat ccacaccggc ttgccgcgcc attcgaccgt
     1021 catcttctcg cccggcttga ggccgctgat gtcggcctcg acggcagcac ccgcggcgcg
     1081 cgcgcgctcg gagggctgga agctggcgac gaagggaacg gcggcaaagc ccgtaccgac
     1141 agcaccggca cagctggagg cgatgatcca ggtgcgcttg ctgctgtcga tttgctggtc
     1201 actcatgggt ttcctcaaca agacttcagg tcagggtcaa cccgcgattc tagcggacgt
     1261 gtcaaggtcc ttgacttcgc cctcgcctgc ggaatactgg gcgcgcgccg tgtccacaag
     1321 aatcaggcgc gaccggcaaa gaacacagga gcgcagcaca tgtctttctc gtcggagttc
     1381 aaagaattcg cgatcaaggg caacgtcatg gatctggcgg tcggcgtgat catcggcgcc
     1441 gcgttcggca agatcgtcga ctcgatcgtc aaggacctga tcatgccggt ggtcggccgc
     1501 gtcttcggcg gcctcgattt ctcgaactac ttcgtgatgc tcgccgcccc gcccgccacc
     1561 tacaacgggc cgatgagcta cgaagcgctg accaaggccg gcgtgccgct gttcgcctac
     1621 ggcaacttca tcaccgtggc gatcaacttc atcatcctgg cgctcatcat tttcgtgatg
     1681 atcaagcagg tcaaccgcct gaagaaggcc tcgccgccgc cggcgccgcc cgccacgccg
     1741 gaaggcatcg tgctgttgcg cgagatccgg gattcgctga agaagtaggc ttgggctgcc
     1801 gcgtcctgcg cgcggcacat cccacgatgc gtcgagcagc ccccctttag accgtcaaat
     1861 ttgggcccat acttcatggg cgcgcgttag gctcgcgtcc acgacgcctg cgttgcagcg
     1921 acacgccatg ctcctgtgcc cgagatgatc ccgatcgaac cgcgattgcg agccgcgctg
     1981 gacgcggcca cgagccagat ccgggcgacc ctgaccgagg tcgcgagcac cctgtcggac
     2041 acgctcggca ccgtcccgca ggcccacccg ggcgaacgcg aactgctcaa ctcggcccgc
     2101 ttcgagctgc ggcgcaacct cgcatcgatg cacctcgcgt tcgaccagga gctgaacaag
     2161 cgcgtcaccg aagacctgac accgcgtgac gagccaaagc gcacgaggct cgccgcgacc
     2221 aactggcagt cgctgaccct ggtcgacgac agcgaggtcg aggggcagat ggtcgccaac
     2281 cgcatcggcc agcagatctg gcacgcctgc gagtccgagc tgcgcgatct ggcgtcctac
     2341 atgggttcgc tgctgaagac cgaccgcagc gaccaggacc gcaacccgct gcgcgccgat
     2401 gtcgtgggtg cgtcgctcta ccgcgcggtc gaggcggtct ccaaccaggc cgaaacgcgc
     2461 aaggtgctgg cgcgcgagtt cggcaacgcg atggccaagg ccatgccggc gtgctacgcc
     2521 gaaatcgtgc gcggcctgca ggcgcgcggc atcgagccgg tgtcgctggc ggtgcgccag
     2581 gtcgacgggc cgggcaacca gctgccgggc ctgaactccg gctacgcgag ccagcgcagc
     2641 gacctggatc cgctctcgtc gctgggtagc ggcagcgcga tgtcgggcta cgagcagcgc
     2701 tcggggcggg gcggcagcag tgggctgcac tccgggccgg gcgcgtcgac ccatggcggc
     2761 tattcgcgct cggggccggg ttcgcaacac gcctcgggcc cggtgctggc cgacgcccag
     2821 ctgatggcgt tgttgcggca cctcactgcg gtcgcgagcc ggcccggcga gctcgatgct
     2881 gccgggctgg gctcgcgcta cggcggggcc ggcgccgccg cggcgggccc gatcgctgct
     2941 g
//
LOCUS       DATMJB010000357         3082 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_143335, whole genome shotgun sequence.
ACCESSION   DATMJB010000357 DATMJB010000000
VERSION     DATMJB010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143335"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="VNU48_07740"
     CDS             complement(<1..161)
                     /locus_tag="VNU48_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906207.1"
                     /note="catalyzes the formation of fumarate from malate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HWI11204.1"
                     /translation="MHTTTIRYADLVESVAGALQYISYYHPADYIAHLARAYEAEQSP
                     AAKDAIAQIL"
     gene            303..>3082
                     /locus_tag="VNU48_07745"
     CDS             303..>3082
                     /locus_tag="VNU48_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWI11205.1"
                     /translation="MSELERWLEGLGLGAHAQAFAEQGIEFDLLPELGDDDLKALGVA
                     QLGHRKRLLKAIVALNAAARMASEPGTLPPTARDAERRQLTVMFCDLVGSTALANRLD
                     PEDLQCVIRSYHAAVTAAVAPYEGYIAQLLGDGVLVYFGFPRAHEDAAVRAVRAALAV
                     LPAVAALKPHADVELQTRIGIATGLVVVGEVGAGTPAAEQSASGETPNLAARLQAHAS
                     PGQIVIADETRHLVGTAFALDPLGHLALKGFAAPVKAWRVLGLRVVASRFEAQHETAL
                     IEFIGRDSEVALLLERWALARDGEGQVVLLTGEAGIGKSRICQTLRERIAGDSAATAL
                     LQCSPYFTGSAFYPVAQHIERTAGIAPSDPPAVRAEKVVRLAGVLSDELLGSVLRMMG
                     LPDGGRTPAASPQQEKAQTAQALGELLQRLAQQQPVLYLVEDAHWIDPSTEELIGQTI
                     ERLREARVLMVVTARPEYAPSWGSPAHLTQLRLSRLGQKQCAALVAAVTGGKPLPAEV
                     LTEIVRKTDGVPLFVEELTKTVLQSGLLVEAPERYELNGPLPPLAIPATLQDSLMARL
                     DRLALAKEVAQAGAVIGREFTRRLLGSVLASLPAPKLDAALDELVRSELVFRRGAALD
                     ATYTFKHALIRDTAYASMLKSQRVLRHAQIAAALEQTESAAAPPELLAHHHQEAGNDE
                     AAVEHWSRAGELAARRSSSVEAQSHYRAAIALLARLEAGPPNQERELRLQILLAQALY
                     QSEGFGGGAYEAATRAAHALARSLDRPDAYVQTGAALVSMMCAAGRLREGIETLEQMR
                     PESLALLPPIGRARRAFNLGMARMQLGELARAWVSVSEALHELETDAETRSPLWAGAD
                     PMVMTLYLCSMVRLRQGMFDQAHEYAARAWQLAQELGHTHSKVVAQMQLGFELWCEGE
                     TAAALPHLTQAL"
BASE COUNT          453 a         1044 c         1136 g          449 t
ORIGIN      
        1 aggatctgcg cgatcgcgtc cttcgcggcc gggctctgct cggcctcgta ggcgcgcgcg
       61 aggtgcgcga tgtaatcggc cgggtggtag tagctgatgt actgcagcgc gccggcgacg
      121 ctctcgacga ggtcggcata acggatcgtg gtggtgtgca tcgtgctctc gcggtgagtc
      181 cggaagaaag gggccaggtg cgattatctc cagccgcccg acccccccag gaaggcgtcg
      241 ggtgcgggcg ccggaacgtg ccccgtgtcg ccgacaatcg cccgcacagg gggaccgcca
      301 cgatgagcga gctggaacgg tggttggaag gtctgggcct cggggcgcac gcgcaggcgt
      361 tcgcggagca ggggatcgag ttcgacctgc tcccggagtt gggcgatgac gacctgaagg
      421 cgctcggcgt ggcccagctc gggcatcgca agcggctgct gaaggcgatc gttgcgctga
      481 acgccgcggc gcgcatggcg agcgaacccg gcacgctgcc gccgacggca cgcgacgccg
      541 agcggcgcca actcaccgtg atgttctgcg acctggtcgg ttcgaccgcg ctcgcgaacc
      601 ggctcgaccc ggaagacctg cagtgtgtga tccgcagcta ccacgccgcc gtcaccgccg
      661 cggtcgcgcc gtacgaaggc tacatcgcgc agctgctcgg cgacggcgtc ctcgtgtact
      721 tcgggttccc gcgcgcccac gaggacgccg cggtccgcgc ggtgcgggcg gcacttgcgg
      781 tgctgccggc ggtcgccgcg ctgaagccgc acgccgatgt cgagctgcag acgcgcatcg
      841 gcatcgccac cggcctcgtc gtcgtcggcg aggtcggcgc cggcacgccg gccgcggaac
      901 aaagcgcgag cggcgagacg ccgaacctcg ctgcgcggct gcaggcgcat gcgtctcccg
      961 gccagatcgt catcgccgac gagacccgcc acctcgtcgg caccgcattc gcgctcgatc
     1021 cgctcgggca cctggcgctg aagggctttg ccgcgccggt caaggcctgg cgcgtgctcg
     1081 gcctgcgcgt ggtggcgagc cgcttcgagg cgcagcacga gaccgcgctc atcgagttca
     1141 tcggccgcga cagcgaggtc gcgctgctgc tcgaacgctg ggccctggcg cgcgacggcg
     1201 agggccaggt cgtgctgctg accggcgagg ccggcatcgg caagtcgcgc atctgccaga
     1261 ccttgcgcga gcgcatcgcc ggcgattcgg cggccaccgc gctgctgcaa tgctcgccgt
     1321 acttcaccgg cagcgcgttc tatccggtgg cgcagcacat cgagcgcacc gccggcatcg
     1381 cgccgtcgga cccgcccgcg gtgcgtgccg agaaggtcgt gcggcttgcg ggcgtgttgt
     1441 ccgacgagct gctcggctcc gtgcttcgca tgatgggcct gcccgacggc ggccgaacgc
     1501 cggccgcatc gccgcagcaa gagaaggcgc agacggcgca ggcactcggc gagttgctgc
     1561 aacgcctggc gcagcagcaa ccggtgctct acctggtcga agacgcgcac tggatcgacc
     1621 ccagcaccga ggagctgatc ggccagacga tcgagcggct gcgcgaggcg cgggtgttga
     1681 tggtcgtcac cgcgcggccc gagtacgcgc cgagctgggg cagcccggcg cacctgacgc
     1741 agctgcgcct gagccggctc ggccagaagc aatgcgccgc gctcgtcgcc gcggtcaccg
     1801 gcggcaagcc cttgcctgcc gaggtgctga ccgagatcgt ccgcaagacc gatggcgtgc
     1861 ccttgttcgt cgaagagctg acgaagacgg tcttgcaatc gggcctgctg gtcgaggcgc
     1921 ccgagcgcta cgagttgaat gggccgctgc cgccgctggc cattcccgcc accttgcagg
     1981 attcgctgat ggcgcgcctg gaccgcctcg ccctggcgaa ggaggtggcg caggccggcg
     2041 cggtgatcgg gcgcgagttc acgcgccggc tgctcggctc ggtgctggcg tcgctgccgg
     2101 cgcccaagct ggacgcggcg ctcgacgaac tggtgcgctc cgagctggta ttccggcgcg
     2161 gtgcggcact cgacgcgacg tacacgttca agcacgcgct gatccgcgac accgcctacg
     2221 ccagcatgct gaagagccag cgcgtgctgc gccatgcgca gatcgccgcc gcgctcgaac
     2281 aaaccgaatc cgccgccgcg ccgcccgaac tgctcgcgca tcaccatcag gaggccggca
     2341 acgacgaagc cgccgtcgag cattggtcac gcgcgggcga gctcgcggcg cggcgctcgt
     2401 ccagcgtcga ggcccagtct cactatcggg cggcgattgc gctgctggcg aggctggaag
     2461 ccgggccgcc caatcaggaa cgcgagctgc gcttgcagat cttgctcgcg caggcgctgt
     2521 atcagagcga aggctttggc ggaggggctt acgaggcggc cacgagggcg gcgcacgcgc
     2581 tggcacgctc gctcgaccgg cccgacgcgt atgtgcagac cggtgcggcg ctcgtcagca
     2641 tgatgtgcgc cgcgggccgg cttcgcgagg ggatcgagac actcgagcag atgcggccag
     2701 aatcgctggc gctgctgccg ccgatcggtc gagcgcggcg ggcattcaac ctgggcatgg
     2761 cgcgaatgca gttgggcgaa ttggctcggg cgtgggtgtc ggtgagcgag gccctgcacg
     2821 agctcgaaac cgacgccgag acgagatcac cgctctgggc cggtgcggac ccgatggtga
     2881 tgacgctcta tttgtgctcc atggtgcgcc tacggcaggg gatgttcgat caggctcacg
     2941 agtatgccgc gcgcgcatgg cagctggcgc aggagctggg tcatacgcat tcgaaggtgg
     3001 ttgcgcagat gcagctgggc ttcgagctct ggtgcgaggg ggaaacggca gcggcgctcc
     3061 cgcatttgac gcaggcgctc ga
//
LOCUS       DATMJB010000358         2976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_781237, whole genome shotgun sequence.
ACCESSION   DATMJB010000358 DATMJB010000000
VERSION     DATMJB010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781237"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VNU48_07750"
     CDS             <1..803
                     /locus_tag="VNU48_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI11206.1"
                     /translation="LYPHMSVFDNMAYGLKIAKLPIAEIKTRVDKAAKILELGPYLQR
                     KPRELSGGQRQRVAMGRAIVRQPQVFLFDEPLSNLDAKLRAQTRLEIQKLHAELGVTS
                     LFVTHDQVEAMTLAQRMLVMNAGRVEQIGTPDEVYHRPASTFVASFIGSPPMNLLTGR
                     ADGARFIAAGQTLALPAAAPRAGEMILGLRPEHAELSRTGTAGWPLKVEALEMLGAER
                     LVYGRVGETLFTVRLDATLAPPKVGDLVTLTATPEHLHWFDAATTKRI"
     gene            806..1570
                     /gene="ugpQ"
                     /locus_tag="VNU48_07755"
     CDS             806..1570
                     /gene="ugpQ"
                     /locus_tag="VNU48_07755"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HWI11207.1"
                     /translation="MADWPYPLWIAHRGAGKLAPENTLAAFRLGAQYGYRAFECDVKL
                     SADGLPFLLHDATLERTTSGSGAAADLAWSELSRLDAGAWHSQGFAGEPLPSLAGIAA
                     YCLHNGFALNIEIKPTPGDEAETGRVVATVAARLWAGQALAPLLSSFQPEALGAARDA
                     APQLPRALLLDSLREGWFEQARALGCVAAVTNHRLMDAALIAQLHGAGMRALVYTVNE
                     APEARRLVALGIDGMITDAVDRFAPALLPTGSGVRD"
     gene            complement(1605..2957)
                     /locus_tag="VNU48_07760"
     CDS             complement(1605..2957)
                     /locus_tag="VNU48_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI11208.1"
                     /translation="MRQVLTRWRTRWRRRFALPATDLLRDATYRRLWTSILISSFGGQ
                     VTMLALPLTAAVLLHASPTQMGLLTAMEIAPFVLFSLPAGVWLDRVRKLPVYIAGESV
                     LSLVVASVPFAWWMGWLAMPWMYFAAFALGTIATVAGSAAQIVLTQVVARERLVEAHA
                     KNALAMSGSEVAGPGLAGALIRMVGAPLALLVDALVLLVSAGILRGIVIHEERRPRAG
                     AHFLRDLRAGMRFVVQHRLLMALAIAVGVWQMCNNAAVVVQILFATRTLGLSAQAVGL
                     SYMAMGAGTIIASVFGSRISRRIGPGPCLVLGFAVCGAGWLLLAAAPANALGVAAFAL
                     MLMLFSTGAVLVFINFLALRQAVTPAPLLGRMTSTMRWLILIPAGPGALLGGWLGEHH
                     GLRSALAFAGSGALLLALLAWRAPVIRNVRTLPRPERERDWLGAEADVRAARPAVEPI
                     "
BASE COUNT          454 a         1118 c         1035 g          369 t
ORIGIN      
        1 cgctgtaccc gcacatgagc gtgttcgaca acatggccta cggtctgaag atcgcgaagc
       61 tgccgatcgc cgagatcaag acgcgggtcg acaaggccgc gaagatcctc gagctcggcc
      121 cctacctgca acgcaagccg cgcgagctct cgggcggcca gcgccagcgc gtcgcgatgg
      181 ggcgcgcgat cgtgcgccag ccgcaggtgt tcctgttcga cgagccgctg tccaacctcg
      241 acgccaagct gcgcgcgcag acgcggctcg agatccagaa gctgcacgcc gagctgggcg
      301 tcacgtcgct gttcgtcacg cacgatcagg tcgaggcgat gacgctggcg cagcgcatgc
      361 tcgtgatgaa cgccggccgc gtggagcaga tcggcacgcc cgacgaggtc taccaccggc
      421 ccgcctcgac ctttgtcgcg agcttcatcg gctcgccgcc gatgaacctg ctcaccggcc
      481 gggccgacgg cgcgcgcttc atcgccgcag gtcagacact ggcattgccg gcggccgcgc
      541 cgcgcgccgg cgagatgatc ctgggcctgc ggcccgagca tgccgagctg agccggaccg
      601 gtaccgccgg gtggccgctg aaggtcgagg cgctcgagat gctgggtgct gagcgcctgg
      661 tctacggccg cgtcggcgag acgctgttca ccgtgcggct ggacgcgacg ctggcgccgc
      721 ccaaggtcgg cgacctggtc acgctgaccg ccacgcccga gcacctgcac tggttcgacg
      781 ccgccacgac gaagcggatc tgacgatggc cgactggccc tacccgctgt ggatcgcgca
      841 ccgaggcgcc ggcaagctgg cccccgagaa cacgctggcc gcgttccgcc tcggcgcgca
      901 atacggctac cgcgccttcg aatgcgacgt gaagctcagc gccgacggcc tgcccttcct
      961 gctgcacgac gccacgctcg aacgcacgac gagcggcagc ggcgccgccg ccgacctcgc
     1021 ctggagcgag ctctcgcggc tcgatgccgg cgcctggcac agccagggct tcgccggcga
     1081 gccgctgccg agcctggccg gcatcgccgc ctattgcctg cacaacggct tcgcgctgaa
     1141 catcgagatc aagccgaccc cgggcgacga agccgagacc ggccgcgtcg tcgccaccgt
     1201 tgccgcgcgc ctgtgggccg ggcaggcgct ggcgccgctg ctcagttcgt tccagcccga
     1261 ggcgctgggg gccgcgcgcg acgccgcgcc gcagttgccg cgcgcgctgc tgctcgacag
     1321 cctgcgcgaa ggctggttcg agcaggcgcg cgcgctcggc tgcgtcgccg cggtgacgaa
     1381 tcaccggctc atggacgccg cgctgatcgc gcagctgcat ggcgccggca tgcgtgcgct
     1441 ggtctacacg gtcaacgagg cgcccgaggc gcggcgcctg gtcgcgctcg gcatcgacgg
     1501 catgatcacc gacgccgtgg accgcttcgc gccggcgctc ttgcccacgg gctcaggcgt
     1561 gcgcgactga cgccgagcag ggggtccatc gtcgtcctcc tgggtcagat cggttcgacc
     1621 gcgggccgcg cggcgcgcac gtcggcctcg gcgcccagcc agtcgcgctc gcgctcgggc
     1681 cgcggcagcg tgcgcacgtt gcggatcacc ggcgcgcgcc aggccagcag cgccagcagc
     1741 agcgcgccgc tgccggcgaa ggcgagcgcc gagcgcagcc cgtggtgctc gccgagccag
     1801 ccgcccagca gcgcgcccgg gccggccggg atcaggatca gccagcgcat cgtgctcgtc
     1861 atccggccca gcagcggcgc cggcgtgacc gcctggcgca gcgcgaggaa gttgatgaac
     1921 accagcaccg cgccggtcga gaacagcatc agcatcagtg cgaacgccgc cacgccgagc
     1981 gcgttcgccg gtgccgcggc cagcagcagc cagcccgcgc cgcagaccgc gaagcccagc
     2041 accaggcatg gccccggtcc gatgcgccgg ctgatgcggc tgccgaagac gctggcgatg
     2101 atcgtgcccg cgcccatcgc catgtagctt aggcccaccg cctgggccga caggccgagc
     2161 gtgcgcgtcg cgaacaggat ctgcaccacg accgcggcgt tgttgcacat ctgccagacg
     2221 cccaccgcga tcgccagcgc catcagcagg cggtgctgca cgacgaagcg catgccggcg
     2281 cgcaggtcgc gcaggaagtg cgcaccggcg cgcggccggc gctcttcgtg aatcacgatg
     2341 ccgcgcagga tgccggccga gaccagcagc acaagcgcgt ccaccagcag cgccagtggg
     2401 gcgccgacca ttctgatcag cgcgccggcc aggcccggcc cggcgacctc ggaacccgac
     2461 atcgccagcg cgttcttcgc atgcgcctcg accagccgtt cgcgcgcgac gacctgcgtc
     2521 agcacgatct gcgcggccga gccggcgacc gtggcgatcg tgccgagcgc gaacgccgcg
     2581 aagtacatcc acggcatcgc cagccagccc atccaccagg cgaacggcac gctcgcgacg
     2641 accagcgaca gcacgctctc gccggcgatg tagaccggca gcttgcgcac gcggtcgagc
     2701 cagacgccgg ccggcagtga gaacagcacg aacggcgcga tctccatcgc ggtgagcaga
     2761 cccatctgcg tggggctggc gtgcagcagc accgcggccg tcagcggcag cgccagcatc
     2821 gtcacctggc cgccgaacga gctgatcagg atcgacgtcc acaggcgccg gtaggtcgcg
     2881 tcgcgcagca ggtcggtggc cggcagcgcg aagcggcgcc gccagcgggt tcgccatctc
     2941 gtcaagacct gtctcatggt cttcacctcg gctttt
//
LOCUS       DATMJB010000359         2240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_771053, whole genome shotgun sequence.
ACCESSION   DATMJB010000359 DATMJB010000000
VERSION     DATMJB010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771053"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..912
                     /locus_tag="VNU48_07765"
     CDS             118..912
                     /locus_tag="VNU48_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HWI11209.1"
                     /translation="MNFGVIIIGDEILSGKRADKHLPKLIELLAARGLTLSWARYVGD
                     DPTRITADLKHAFASGDVVFSCGGIGATPDDHTRQCAAAALGVPLALHPQARELILER
                     MRDIAREQGIPYEPERDDNVHRLNMGVFPEGARIIPNPYNKIAGFSVGHVHFVPGFPV
                     MAWPMIEWVLDQHYPLLHGQGARPEKSVIVFGAMEATLTPLMERIEAQFVGVKVFSLP
                     SVDHPEWGRHIELGVKGPADQLDAAYRVLLEGLAAHGAKLGPELVR"
     gene            1144..>2240
                     /gene="glnA"
                     /locus_tag="VNU48_07770"
     CDS             1144..>2240
                     /gene="glnA"
                     /locus_tag="VNU48_07770"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HWI11210.1"
                     /translation="MASKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPVSHFDE
                     DKFTGGHAFDGSSIAGWKGIEASDMQLMPDPNTANIDPFFEEPTMILTCDVVEPGDGK
                     PYERDPRSLAKRAEAYMKASGLGDTAFFGPEPEFFVFDSVRWGNDMSGCFSKIESEEA
                     AWNTGKEYEHGNSGYRPSVKGGYFPTPPVDSFQDMRSEMCLVLESLGIPCEVHHHEVA
                     NAGQMELGTKFSTLLQRADWVQLQKYVVHNVANAYGKTATFMPKPIVGDNGSGMHVHQ
                     SVWKDGKNLFAGDGYAGLSEFALFYIGGIIKHARALNAITNPGTNSYKRLVPGYEAPV
                     KLAYSSKNRSASIRIPHVANPKGRRVEARFP"
BASE COUNT          395 a          763 c          734 g          348 t
ORIGIN      
        1 cctgccgccg gtgcggcacg gcgcgcgtgc cgccgagccg gtcgacgagg tccgcgtgat
       61 cgcgccgccg cgcgccggcg cggacgatgc ctttcgctcc ccaccgagga ccggaccatg
      121 aacttcggcg tgatcatcat cggcgacgag atcctgtcgg gcaagcgtgc cgacaagcat
      181 ctgcccaagc tcatcgagct gctggccgcg cgcggcttga ccctgagctg ggcgcgctac
      241 gtcggcgacg acccgacgcg catcaccgcc gacctgaagc acgcgttcgc gtccggcgac
      301 gtggtgttct cgtgcggcgg catcggcgcc acgcccgacg accacacgcg ccagtgcgcg
      361 gccgcggcgc tgggcgtgcc gctcgcgctg cacccgcagg cgcgcgagct gatcctcgag
      421 cgcatgcgcg acatcgcgcg cgagcagggc atcccgtacg agccggagcg cgacgacaac
      481 gtgcaccggc tgaacatggg cgtgttcccc gaaggcgcgc ggatcatccc gaacccgtac
      541 aacaagatcg ccggcttcag cgtcggccat gtgcacttcg tacccggctt cccggtcatg
      601 gcctggccga tgatcgagtg ggtgctcgac cagcactacc ccttgctgca cgggcagggc
      661 gcacgacccg agaaatcggt gatcgtgttc ggcgcgatgg aggccacgct gacgccgctg
      721 atggagcgca tcgaagcgca gttcgtgggc gtcaaggtct tcagcctgcc cagtgtcgac
      781 caccccgaat ggggccgcca catcgaactc ggcgtgaagg gcccggccga tcaactcgac
      841 gcggcctacc gcgtgctgct cgaagggctg gccgcgcatg gcgccaagct gggccctgag
      901 ttggtgcgat agtaggcgcg ctactggtgc gctcatctgc cctcacgtgg ggcatctcgc
      961 gcgcctgcgc ttgtcgctcg acgccccgtg gggccagggg gtttttcagg tcggtgcggc
     1021 gccggtcgcg ggcatgcttt ctgctacatt cctgtgcgcg ttttttgggc ctgggagcag
     1081 gccttctgaa ggccgaagcc cacaattctc aatcccgaac aagcatccca ccaggagttc
     1141 cagatggcca gcaagaccgt tgccgacgtg atgaagatgg tgaaggagaa cgaagtcaag
     1201 ttcgtcgact tccgcttcac cgacacccgc ggcaaagagc agcacgtgac cgtgccggta
     1261 tcgcacttcg acgaagacaa gttcaccggc ggccacgcct tcgacggctc gtcgatcgcc
     1321 ggctggaagg gcatcgaggc ctcggacatg cagctgatgc ccgacccgaa caccgccaac
     1381 atcgatccgt tcttcgaaga gccgacgatg atcctgacct gcgacgtggt cgagcccggc
     1441 gacggcaagc cctacgagcg cgacccgcgt tcgctggcca agcgcgccga agcctacatg
     1501 aaggcctcgg gcctgggcga caccgcgttc ttcggccccg agcccgagtt cttcgtgttc
     1561 gacagcgtgc gctggggcaa cgacatgtcg ggctgcttct cgaagatcga atcggaagag
     1621 gccgcatgga acaccggcaa ggaatacgag cacggcaact cgggctaccg cccgagcgtg
     1681 aagggcggct acttcccgac cccgccggtc gattcgttcc aggacatgcg ctccgagatg
     1741 tgcctggtgc tcgagtcgct gggcatcccg tgcgaagtgc atcaccacga ggtcgcgaac
     1801 gccggccaga tggagctcgg cacgaagttc agcacgctgc tgcagcgcgc cgactgggtc
     1861 cagctgcaga agtacgtggt ccacaacgtg gccaacgcct acggcaagac ggcgaccttc
     1921 atgcccaagc cgatcgtcgg cgacaacggc agcggcatgc acgtgcacca gtcggtctgg
     1981 aaggacggca agaacctgtt cgccggtgat ggctatgcgg gcctgagtga atttgccctc
     2041 ttctacatcg gcggcatcat caagcacgcg cgcgcgctga acgcgatcac gaacccgggc
     2101 accaactcgt acaagcgcct cgtgccgggc tacgaagcgc cggtcaagct cgcgtactcg
     2161 agcaagaacc gctcggcgtc gatccgcatc ccgcacgtcg cgaacccgaa gggccggcgt
     2221 gtcgaggcgc gtttcccgga
//
LOCUS       DATMJB010000360         2827 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_544156, whole genome shotgun sequence.
ACCESSION   DATMJB010000360 DATMJB010000000
VERSION     DATMJB010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544156"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..137
                     /locus_tag="VNU48_07775"
     CDS             <1..137
                     /locus_tag="VNU48_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="VF530 family DNA-binding protein"
                     /protein_id="HWI11211.1"
                     /translation="INSFNTDPSIRSSLTFLRKTPWAREKVEGLYLFMQREQARARRG
                     "
     gene            153..2093
                     /locus_tag="VNU48_07780"
     CDS             153..2093
                     /locus_tag="VNU48_07780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWI11212.1"
                     /translation="MTAPTPTPAPFGSWPSPVTAELIAGATIRLGQIALRGSDVLWSE
                     GRPQEQGRNVLVQRSPDGTLRDLNPAPFNVRTRAHEYGGGAFVALPDGIVFSHYDDQQ
                     LYRIDATGAPRQLSFEPDQRYADAIVDAQRQRLIAVREDHRAGAVDAVATLVAIALDG
                     GTIEVLAQGHDFFASPALSPDGRRLAWLSWDHPNMPWDGNELWLAELEADGTLAAPRR
                     VAGGPSESIFQPAWSPRGELHFVSDRSGWWNLYRERDGIVQPLHPMAAEFGVPQWAFG
                     MSSYGFDARGRIVCSYQQGGLSHLALLDAEADTFETLPSPYCAIDGLRVGADFAVFLG
                     ASATEPSALLRLDLTTRAFEVLRASSTAQVDAAGLSRAEAITFPTEGGLRSHAFFYAP
                     ANRDRRGPDGERPPLIVVSHGGPTGATDAAFKWSLQFWTSRGFAVVDVNYGGSSGYGR
                     AYRERLNGQWGVVDVDDSVNAARFLIARGDVDAQRVVIRGASAGGYTTLCALTFRDFF
                     KAGASHYGIGDLEALVRDTHKFESRYLDRLIGPYPQQRARYRERSPIHHTDRLARPMI
                     LLQGAEDKAVPPAQAQTMYDAVAAKGLPVAYLLFEGEQHGFRRAATIKRALEAELYFY
                     GKVFGFTPADAIEPVEIRNLRR"
     gene            2210..2698
                     /locus_tag="VNU48_07785"
     CDS             2210..2698
                     /locus_tag="VNU48_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11213.1"
                     /translation="MKSSWRTAWQGQDIVVLRNEVEVDRLHAPDIERVVLVHRGSGDS
                     PGDLVQAVVEIGDACLIFPADTGFAGRVNFERQAFWADKACVYWVNESRAPLPLRLRR
                     GRWFLGLTHPVFTRVPRTELAALLERWPVQGPQTWEQRKWRRIELSRPFAADTPQARL
                     RA"
BASE COUNT          415 a         1032 c          985 g          395 t
ORIGIN      
        1 agatcaacag cttcaacacc gacccgagca tccgctcgag cctgaccttc ctgcgcaaga
       61 cgccgtgggc gcgcgagaag gtcgagggcc tgtacctgtt catgcagcgc gagcaggcgc
      121 gcgcccggcg cggctgaccg ccggcaccca cgatgaccgc accgaccccc acgcccgcgc
      181 cgttcggcag ctggccctcg cccgtcaccg ccgaactgat cgccggcgcg acgattcgcc
      241 tcggccagat cgcgctgcgc ggcagcgacg tgctctggag cgaaggccgg ccgcaggaac
      301 agggccgcaa cgtgctggtg cagcgcagcc ccgacggcac gctgcgcgac ctgaacccgg
      361 cgccgttcaa cgtgcgcacc cgtgcgcacg agtacggcgg cggcgcgttc gtcgcgctgc
      421 ccgatggcat cgtcttcagc cactacgacg accagcagct ctaccgcatc gacgcgaccg
      481 gcgcgccgcg ccagctgagc ttcgagccgg accagcgcta cgccgatgcg atcgtcgatg
      541 cgcagcgcca gcgcctgatc gcggtgcgcg aggaccaccg cgccggcgcc gtcgacgcgg
      601 tcgcgacgct ggtggcgatc gcgctcgacg gcggcacgat cgaggtgctc gcgcaaggcc
      661 acgacttctt cgccagcccg gcgctgagcc ccgacggccg ccggctcgca tggctgagct
      721 gggaccaccc gaacatgccc tgggacggca acgagctctg gctcgccgag ctcgaggccg
      781 acggcacgct ggccgcgccg cgccgcgtcg ccggcggccc gagcgaatcg atcttccagc
      841 ccgcgtggtc gccgcgcggc gaactgcatt tcgtcagcga ccgcagcggc tggtggaacc
      901 tgtaccgcga gcgcgacggc atcgtgcagc cgttgcaccc gatggcggcc gagttcggcg
      961 tgccgcagtg ggccttcggc atgtcgagct acggcttcga tgcgcgcggg cgcatcgtgt
     1021 gcagctacca gcagggcggc ctgtcgcatc tggcgctgct cgatgccgag gccgacacct
     1081 tcgagacctt gccgagcccg tactgcgcga tcgacgggct gcgcgtcggc gccgacttcg
     1141 cggtcttcct cggcgccagt gcgaccgagc cgagcgcgct gctgcggctc gacctgacca
     1201 cgcgcgcgtt cgaggtgctg cgcgcgtcga gcacggcgca ggtcgacgcg gccggcctgt
     1261 cgcgcgccga ggcgatcacc ttcccgaccg aaggcgggct gcgctcgcac gcgttcttct
     1321 acgcaccggc gaaccgcgat cgccgcgggc ccgacggcga acgcccgccg ctgatcgtcg
     1381 tcagccacgg cgggccgacc ggcgccaccg atgccgcgtt caagtggtcg ctgcagttct
     1441 ggaccagccg cggcttcgcc gtcgtcgacg tgaactacgg tggcagcagc ggctacggcc
     1501 gcgcctaccg cgagcggctg aacgggcaat ggggcgtggt cgatgtcgac gactcggtca
     1561 acgcggcgcg cttcctgatc gcgcgcggcg atgtcgacgc gcagcgtgtc gtgatccgcg
     1621 gcgccagcgc cggcggctac acgaccctgt gcgcgctgac cttccgcgac ttcttcaagg
     1681 ccggcgcgag ccactacggc atcggcgacc tcgaggcgct ggtgcgcgac acccacaagt
     1741 tcgagtcgcg ctacctcgac cggctgatcg gcccgtaccc gcagcagcgc gcgcggtatc
     1801 gtgaacgctc gccgatccac cacaccgatc gcctggcccg cccgatgatc ctgctgcagg
     1861 gcgccgagga caaggcggtg ccgccggcgc aagcgcagac gatgtacgac gcggtcgcgg
     1921 ccaaggggct gccggtggcg tacctgctgt tcgagggcga gcagcacggc ttccggcgcg
     1981 ccgcgacgat caagcgcgcg ctcgaggccg agctgtactt ctacggcaag gtgttcggct
     2041 tcacgccggc ggacgcgatc gagccggtcg agatccgcaa cctgcgccgc tgaccgggcg
     2101 aacgtccgct tgcaaattga tgcgactgct gacagcaagc caagggcgcg cgccttagcg
     2161 ttggcgaccc acgaagtcga tgggaacgaa aaccgaggga agcaacgcaa tgaagtcgtc
     2221 gtggcgcacc gcctggcaag ggcaggacat cgtggtcctg cgcaacgagg tcgaggtcga
     2281 tcggctgcac gcacccgaca tcgaacgcgt cgtgctggtg catcgcggct ccggcgattc
     2341 gcccggcgac ctggtgcagg cagtggtcga gatcggcgac gcctgcctga tcttcccggc
     2401 cgacaccggc ttcgccggcc gcgtcaattt cgaacgccag gcgttctggg ccgacaaggc
     2461 ctgcgtctac tgggtcaacg agtcacgcgc accgctgccg ctgcggctgc gccgcggccg
     2521 ctggttcctg ggcctgacgc acccggtgtt cacgcgcgtg ccgcgcaccg aactcgccgc
     2581 gctgctcgag cgctggccgg tccaggggcc gcagacctgg gagcagcgca aatggcgccg
     2641 gatcgagctc agccgcccgt tcgcggccga cacgccgcag gcgcgtctgc gcgcctgagc
     2701 gtgactcctt tcgcgcccca agaatgaaca aacccggccg aggccgggtt tgtcgttcag
     2761 tgcggggtcg ctcaggcggc cggcttcgca gcagcagcct tcttcttggc gtgcttcttg
     2821 gccgggg
//
LOCUS       DATMJB010000361         3715 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_416461, whole genome shotgun sequence.
ACCESSION   DATMJB010000361 DATMJB010000000
VERSION     DATMJB010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416461"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VNU48_07790"
     CDS             complement(<1..243)
                     /locus_tag="VNU48_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HWI11214.1"
                     /translation="MLASLAAASSAEPIGSVDTVFKLIGPDHKIVVDAYDDPKVGGVT
                     CYVSRAKTGGIKGAIGLAEDKSEASIACRQVGAISFG"
     gene            complement(306..998)
                     /locus_tag="VNU48_07795"
     CDS             complement(306..998)
                     /locus_tag="VNU48_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HWI11215.1"
                     /translation="MIEVYSWATPNGHKVHIMLEECGLPYHVHAINIGAGDQFAPEFL
                     AISPNNKIPAIVDPQGPDGAPISLFESGAILLYLAGKAGRFMPAGTRERYAMLEWLMF
                     EMGSVGPMLGQTHHFRLYAPEKIEYAVNRYTNEARRLYGVMDKRLASVKYIAGAQYTI
                     ADIAIFPWLRSWKNQGIDWNDYPHLKGWFDEIAARPAVQRGVEVLAAQRKPLVDEQAR
                     EMLFGATQYKKH"
     gene            1074..1826
                     /locus_tag="VNU48_07800"
     CDS             1074..1826
                     /locus_tag="VNU48_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11216.1"
                     /translation="MLESFKRLFAKPGNDRDYTPISEWAQRRGFGFKRVRDEDGFVID
                     GAFDKRPWRIEWGPPQREYIEGHELRLRMELDLPSDMQMLLLSRPLMDALERATFEEF
                     TDDVQTQIGTKTPEEMRWLVMFPKVNLSTLKSLRLRFGAVASVPASGLSWIEGTLAER
                     LEAASIDLLRDEPPFVLMTLRGRTYLRMQLESPQPQALAAALALFETAVAQALRAASG
                     ATDSGVDWPATGATTAWQSYEPDPPAVPPKRR"
     gene            complement(1866..3107)
                     /locus_tag="VNU48_07805"
     CDS             complement(1866..3107)
                     /locus_tag="VNU48_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI11217.1"
                     /translation="MDFAYSPRALELQARLLKFMDEHVYPAEAKYEAEIAANTAAGKR
                     WTPLNTIEALKPKARAIGLWNLFLPPTAGGAGSAHNGRDFGLSNSDYAPLAEIMGRVP
                     WCSEVFNCSAPDTGNMETIERYGKPEHKAQWLEPLLNGKIRSAFAMTEPAVASSDATN
                     IASRIERQGDDYVINGRKWWISGAGDPRCQIYIFMGKTDPEAPRHAQQSMILVPANTP
                     GIKVLRPLSVFGYDDAPHGHMEITFDDVRVPASNILLGVGRGFEIAQGRLGPGRIHHC
                     MRLIGLAERSLELMCKRASSRVAFGRTVAEQGVTRERIAEARCKIDMARLLTLKAAWI
                     MDVAGNKTAKAEIAMIKVVAPSMALQVIDWAMQAHGGGGVCGDFPLAGFYAGARTLRF
                     ADGPDEVHRNAIAKLELGKYA"
     gene            complement(3202..>3715)
                     /locus_tag="VNU48_07810"
     CDS             complement(3202..>3715)
                     /locus_tag="VNU48_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWI11218.1"
                     /translation="AMDRLTEWLPAHMPASARDASQVSIVHGDYRLDNLVFHATEPRI
                     IAVLDWELSTIGHPLADFSYHCMSWHIHHTLGRGIGGHDLAALGIPDEKSYVRRYCER
                     TGRLDPDAVMADWNFYIAYNLFRMAGILQGIAKRVEMGTASSAQARQAGAGARPLADM
                     GWAIARNDSP"
BASE COUNT          546 a         1231 c         1286 g          652 t
ORIGIN      
        1 gccgaagctg atggcgccga cctggcggca ggcgatcgag gcctcggact tgtcttcggc
       61 caggccgatc gcgcccttga tgccgccggt cttggcgcgc gacacgtagc aggtcacgcc
      121 gccgaccttc gggtcgtcgt aggcatcgac gacgatcttg tggtcggggc cgatcagctt
      181 gaacacggtg tcgaccgagc cgatgggttc ggcactcgac gcggccgcca ggctcgcgag
      241 catcaggcct gcaacgggga aaagggaagt cttcacggca atccttgtct cgagccgcgg
      301 cgcggtcagt gctttttgta ctgcgtcgcg ccgaacagca tctcgcgcgc ctgctcgtcc
      361 accagcggct tgcgctgcgc tgccagcacc tcgacgccgc gctgtaccgc cgggcgcgcg
      421 gcgatctcgt cgaaccagcc tttcaggtgc gggtagtcgt tccaatcaat gccctggttc
      481 ttccacgagc gcagccacgg gaagatcgcg atgtcggcga tcgtgtactg cgcgccggcg
      541 atgtacttga cgctggccag ccgcttgtcc atcacaccgt acaaccgtcg cgcctcgttg
      601 gtgtagcggt tcaccgcgta ctcgatcttc tcgggcgcat acaggcggaa gtggtgcgtc
      661 tgcccgagca tcgggccgac gctgcccatc tcgaacatca gccactcgag catcgcgtag
      721 cgctcgcgcg tgcccgccgg catgaaccgg ccggccttgc cggccaggta cagcaggatg
      781 gcgcccgact cgaacaacga gatcggcgcg ccgtcgggcc cctgcgggtc gacgatcgcg
      841 gggatcttgt tgttcgggct gatcgccagg aactcgggcg cgaactggtc gccggcgccg
      901 atgttgatcg catgcacgtg gtacggcagg ccacactctt cgagcatgat gtgtactttg
      961 tgcccgttcg gcgtggccca cgaatacact tcgatcatgt tcgcgttcat gtggcaggtt
     1021 cccggatgcg caagcgcgtg actggcattc actctagttg attgggcgtt ccgatgctcg
     1081 aatcgttcaa gcgcttgttc gccaaacccg ggaatgaccg ggactacacg ccgatctcgg
     1141 agtgggcgca gcgccgcggt ttcggcttca agcgcgtgcg cgacgaagac ggcttcgtga
     1201 tcgacggtgc gttcgacaag cggccctggc gcatcgagtg gggcccgccg cagcgcgagt
     1261 acatcgaagg gcacgagctg cgcctgcgca tggagctcga cctgccgtcg gacatgcaga
     1321 tgctgctgct gtcgcgcccg ctgatggacg cgctcgaacg cgcgaccttc gaggaattca
     1381 ccgacgacgt gcagacccag atcggcacca agacgcccga agaaatgcgc tggctggtga
     1441 tgtttccgaa ggtcaacctg tcgacgctga agtcgctgcg gctgcgcttc ggcgcggtcg
     1501 cgagcgtgcc ggcgtcgggc ctgtcgtgga tcgagggcac gctggccgag cggctcgaag
     1561 cggcctcgat cgacctgctg cgcgacgagc cgccgttcgt gctgatgacc ttgcgcgggc
     1621 gcacctatct gcgcatgcag ctggagtcgc cgcagccgca ggcgctggcc gcggcgctgg
     1681 cgctgttcga gaccgcggtt gcgcaggcgc tgcgcgccgc cagcggggcg accgacagcg
     1741 gcgtggactg gcccgcgacc ggtgccacga ctgcatggca gagctacgag ccggacccgc
     1801 cggcggtgcc gcccaagcgg cgctgaccgc ggggcgaagg gcgctcacgc gcccacgcga
     1861 ccggttcacg cgtacttgcc gagttccagc ttcgcaatcg cgttgcggtg cacttcgtcc
     1921 ggcccatcgg cgaagcgcag ggtgcgcgcg ccggcgtaga agccggcgag cgggaagtcc
     1981 ccgcagacgc cgccgccgcc gtgcgcctgc atcgcccagt cgatcacctg cagcgccatg
     2041 ctcggtgcga cgaccttgat catcgcgatc tcggccttcg cggtcttgtt gccggcgacg
     2101 tccatgatcc acgcggcctt cagcgtcagc agccgcgcca tgtcgatctt gcagcgcgcc
     2161 tcggcgatgc gctcgcgcgt cacgccctgt tcggcgacgg tgcggccgaa cgcgacgcgc
     2221 gacgatgcgc gcttgcacat cagttcgagc gagcgctcgg ccaggccgat caagcgcatg
     2281 cagtggtgga tgcgccccgg cccgaggcgg ccttgggcga tctcgaagcc gcggccaacg
     2341 cccagcagga tgttgctggc cggcacgcgc acgtcgtcga aggtgatctc catgtggccg
     2401 tgcggcgcgt cgtcgtagcc gaacaccgac agcgggcgca gcaccttgat accgggtgtg
     2461 ttcgccggca ccaggatcat cgactgctgc gcgtggcgcg gcgcctcggg gtcggtcttg
     2521 cccatgaaga tgtagatctg gcagcgcgga tcgccggcgc cgctgatcca ccacttgcgg
     2581 ccgttgatga cgtagtcgtc gccctggcgc tcgatgcgcg aggcgatgtt ggtcgcatcg
     2641 gacgaggcca ccgcgggttc ggtcatcgcg aaggcgctgc ggatcttgcc gttcagcagc
     2701 ggctcgagcc actgcgcctt gtgttcgggc ttgccgtagc gctcgatcgt ctccatgttg
     2761 ccggtgtcgg gcgccgagca gttgaagacc tcggagcacc agggcacgcg gcccatgatc
     2821 tcggccagcg gcgcgtagtc gctgttgctc aggccgaagt cgcggccgtt gtgcgccgag
     2881 cccgcgccgc cggcggtggg cggcaggaac aggttccaca gaccgatcgc gcgtgccttc
     2941 ggcttgagcg cctcgatcgt gttcaacggc gtccagcgct tgccggcggc ggtgttcgcg
     3001 gcgatctccg cctcgtactt ggcctcggcc gggtagacgt gctcgtccat gaacttcagc
     3061 agccgggctt gcagttcgag ggcacggggt gagtaggcga aatccatcat ggtctccttc
     3121 gtgaatctgt ggtcaggtgc tcagccgagc gcggccgccc caagccggct gagttcccct
     3181 tggggacggt gccgcaggcg cctaggggct gtcatttctc gcgatcgccc agcccatgtc
     3241 ggccaggggt cgcgcaccgg ccccagcttg acgtgcttgc gcgctcgacg ctgtccccat
     3301 ctcgacacgt tttgcaattc cttgcaggat gccggccatg cggaacaggt tgtaggcgat
     3361 gtagaagttc cagtcggcca tcaccgcgtc gggatcgagg cggccggtgc gctcgcagta
     3421 gcgccgcacg taactctttt cgtcggggat gccgagcgcg gcgaggtcgt gcccgccgat
     3481 gccacggccc agcgtgtgat gaatgtgcca ggacatgcag tggtagctga agtcggcgag
     3541 cggatggccg atggtcgaca gctcccagtc gagcaccgcg atgatgcgcg gctcggtcgc
     3601 atggaacacc aggttgtcga gccggtagtc gccatgcacg atcgacacct ggctcgcgtc
     3661 gcgtgcgctt gccggcatgt gcgcgggcag ccactcggtg agccggtcca tcgcc
//
LOCUS       DATMJB010000362         2056 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_206699, whole genome shotgun sequence.
ACCESSION   DATMJB010000362 DATMJB010000000
VERSION     DATMJB010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206699"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VNU48_07815"
     CDS             complement(<1..400)
                     /locus_tag="VNU48_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWI11219.1"
                     /translation="MRVNWLAWSAVDSVGGVILAFAVTVLFARVLEPADFGRFALALG
                     LVQLASVATEALLHDAVVQRQELEDAHAHTAVWTIAALGLALMLLMLLGAHRFADLAG
                     DPSIAPLVGALALSIPLAGVSSVLTAQLRRA"
     gene            475..1422
                     /locus_tag="VNU48_07820"
     CDS             475..1422
                     /locus_tag="VNU48_07820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWI11220.1"
                     /translation="MEEPHAITPTFTVLMPVYNGAAFLRAAIDSVIGQSRADWELIVV
                     DDGSTDESAAVAAEAAGRDARVRLLRKPNTGISDTLNLGLAAARGEWIARLDCDDEME
                     PRRLARQLEFLAARPDLGGAASYYTVINRDGHARQQIRPLPLSVDEIEPLFARGGTLR
                     YTHPTVTYRREVAHALGGYRRELEPCEDVDLFVRFFEAGRPVVVQPEFLTRYRAHPGQ
                     ITASKLHDGFNKVLFIHDNFFRRRAGRPERSYAEFLTSLRTAPLAERVRHFADYGAEI
                     LHRRSTALLMADRTFAGYLCLAGAGALRPVRAARRLLRR"
     gene            1738..>2056
                     /locus_tag="VNU48_07825"
     CDS             1738..>2056
                     /locus_tag="VNU48_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11221.1"
                     /translation="MESLKRRVLALSDCIDKSSPIVLVDYHVSTNLGDLLIHVGSDEF
                     FRAHGYRVAAEYARQNFLHDHTPIPDRATIVFEAGGNTGDLYPAHQELRERIIARRHG
                     NRIV"
BASE COUNT          319 a          777 c          675 g          285 t
ORIGIN      
        1 gcgcgcgccg cagctgcgcg gtgaggacgc tcgacacgcc ggccagcggg atcgacagcg
       61 cgagcgcccc gaccagcggc gcgatcgagg gatcgccggc gagatccgcg aagcggtgcg
      121 cgccgaggag catcagcagc atcagcgcga ggccgagcgc ggcgatcgtc cacaccgcgg
      181 tgtgcgcgtg cgcgtcctcg agctcttggc gctgaaccac cgcgtcgtgc agcagcgcct
      241 cggtcgcgac gctggcgagc tggacgaggc cgagcgcgag cgcgaagcgg ccgaaatcgg
      301 ccggctcgag cacccgcgcg aacagcacgg tcaccgcgaa ggcgaggatc acgccaccca
      361 cgctgtcgac cgccgaccac gccagccaat tgactcgcat cgcgcgccgc tgctccgtcc
      421 gtcgcccgac ttcctacccg gttgcccggc gcgaagatag ggtcggctcc gcgcatggaa
      481 gaaccgcacg cgatcacgcc caccttcacg gtgctgatgc cggtctacaa cggcgcggcg
      541 ttcctgcgcg ccgcgatcga cagcgtcatc ggccagagcc gcgccgactg ggagctcatc
      601 gtcgtcgacg acgggtccac cgacgaatcc gcggcggtcg ccgccgaagc cgcggggcgc
      661 gacgcgcgcg tccgcctgct gcgcaagccc aacaccggga tcagcgacac actcaacctc
      721 ggcctcgccg cggcgcgggg agagtggatc gcgaggctcg actgcgacga cgagatggag
      781 ccgcggcgac tcgcgcgcca gctcgagttc ctcgccgcgc ggcccgatct cggcggcgcc
      841 gcgtcctact acacggtgat caaccgcgac ggccatgcgc gccagcagat ccggccgctg
      901 ccgctctccg tcgacgagat cgagccgctg ttcgcccgcg gcggcacgct gcgctacacg
      961 cacccgaccg tcacctatcg ccgcgaggtc gcccacgcgc tcggcggcta tcgccgggaa
     1021 ctcgagccct gcgaggacgt tgatctgttc gttcggttct tcgaggcggg gcgtcccgtc
     1081 gtcgtccagc ccgagttcct cacccgctat cgcgcgcatc ccgggcagat caccgccagc
     1141 aagctccacg acggattcaa caaggtgcta ttcatccacg acaatttttt tcgccggcgc
     1201 gccggacggc cggaacggag ctacgccgag ttcctcacat cgctgcgcac cgcgccgctc
     1261 gcggagcgcg tgcggcactt cgccgactac ggcgccgaga tcctccatcg ccgctcgacc
     1321 gcgctgctga tggccgaccg gacgttcgcg ggctatctct gcctcgccgg cgccggcgcg
     1381 ctgcggcccg tgcgcgcggc gcgccgcctg ctccgtcgat gacgcgggcg atgggctccg
     1441 cggtcgaact cagcgacgcg cgccgatgcg ccgccgcgac gcgcggcgcg ctgcgcagcg
     1501 tgcaaggctc gggccgcgag gtgcccgcat ttcgtcgacc gaatctctcg agcgcgtcgt
     1561 cataccggct tgccaatcga ggccgcggcc gccacgattg cgttccgatt ccccgtcacg
     1621 acagcgcgcc gcagcccgga ggatccgaac gcgagcggat tcggctgcgg caccattcgt
     1681 gaaactgtca cacacgttcg cttaaggtac ccgtcttcga tgcgtcatgc cgaggtcatg
     1741 gaatcgctca agcgccgcgt gctcgcgctc agcgactgca tcgacaagtc gtccccgatc
     1801 gtgctggtcg actaccacgt cagcacgaat ctcggcgacc tgctcatcca tgtcggctcg
     1861 gacgagttct ttcgcgccca cggctaccgc gtggccgcgg aatacgcgcg ccagaacttc
     1921 ctgcacgacc acacgccgat ccccgaccgc gcgacgatcg tcttcgaggc cggcgggaac
     1981 accggcgatc tctacccggc gcaccaggag ttgcgcgagc ggatcatcgc gcggcgccac
     2041 ggcaacagga tcgtca
//
LOCUS       DATMJB010000363         2563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_683980, whole genome shotgun sequence.
ACCESSION   DATMJB010000363 DATMJB010000000
VERSION     DATMJB010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683980"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VNU48_07830"
     CDS             <1..452
                     /locus_tag="VNU48_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-containing monooxygenase EthA"
                     /protein_id="HWI11222.1"
                     /translation="LDADIIVTATGLALELAGGLQVSVDGVPVDWPATLNYKGLMYSG
                     VPNFAAVFGYTNASWTLKADLISEYVCRLLNHMATHGLRQCTPRIADPLMPTEPWVDF
                     SSGYLQRELHRFPKQGTRGPWKLNQNYLRDLFALRYGRLDDGAMTFA"
     gene            complement(413..1180)
                     /locus_tag="VNU48_07835"
     CDS             complement(413..1180)
                     /locus_tag="VNU48_07835"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HWI11223.1"
                     /translation="MADTPPPYVHEELDARSLHFSTSAIQSRMQLRRPDALELEYTRL
                     MMGWPMFRPQARRLAMIGLGGGSIAKFCHRHLPRATMLVVEIDPRVIALREAFLVPAD
                     DARFRVVEGDGAQLIATTEERFDVLMVDAFDADGMPAALGTQRFYDDCLDALATDGLF
                     VVNLHAGHPQHAVYVDRIARSFGQRVLRVTDRDGSNTVVFAAKGDAIARAGGASLRKP
                     AALAEAPWSELRGAFSRIASALQRDQANVIAPSSSRP"
     gene            complement(1190..1636)
                     /locus_tag="VNU48_07840"
     CDS             complement(1190..1636)
                     /locus_tag="VNU48_07840"
                     /inference="COORDINATES: protein motif:HMM:NF027434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4826 family protein"
                     /protein_id="HWI11224.1"
                     /translation="MTSSDAGEEERWCDARAVEVAACADRLAPAHGRIGERPAWFAMP
                     YASLWAVESAQRPEWIGWWVIAGDLPTDAIAAHELPTPRDALRAFGKRWVLHGEALDR
                     GDVPPAWAHLPDDALPKLAAQLKRRGAALQVWADDAAAWPVDESED"
     gene            complement(1638..2528)
                     /locus_tag="VNU48_07845"
     CDS             complement(1638..2528)
                     /locus_tag="VNU48_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWI11225.1"
                     /translation="MPELQTLQPHQKDLGGGFIVRRSLPSAQRQAVGPFVFFDHFGPI
                     TAKPDDNFDVRPHPHIGLATVTYLFEGAMMHRDSTGVVQRIEPGAINLMTAGRGIVHS
                     ERTPKDLLGIERRSHGLQLWLAVPTEREEDAPGFQHVPGAEIPARDVDSANAQVLVRV
                     LIGSAFGLSSPVRTLSPTLYLDLNFEIASGAVLRIPDAAEERALYSVDHDFELDGELV
                     PAFTMVVLEPGSEPLLSAPRGGHIVLIGGTPLGHRFMAWNFVSSRKERIAQAQDDWEA
                     QRFERVPGETEFIPLPPRRP"
BASE COUNT          389 a          891 c          902 g          381 t
ORIGIN      
        1 agctcgatgc cgacatcatc gtcaccgcaa ccggcctcgc gctcgaactg gcgggcggcc
       61 tgcaggtcag cgtcgacggc gtgccggtcg actggccggc cacgctgaac tacaagggcc
      121 tgatgtacag cggcgtgccg aacttcgccg cggtgttcgg ctacacgaac gcatcgtgga
      181 cgctgaaggc cgacctgatc tccgagtacg tctgccggct gctgaaccac atggcgacgc
      241 acgggttgcg ccaatgcacg ccgcgcatcg ccgacccttt gatgcccacc gagccgtggg
      301 tcgatttctc gtcgggctat ctgcagcggg agttgcaccg ctttccgaaa cagggcacgc
      361 gcgggccctg gaagctgaac cagaactact tgcgcgacct gttcgcgctg cgctacggcc
      421 ggctcgacga cggagcgatg acgttcgcct gatcgcgctg cagggcactt gcgatgcgcg
      481 agaacgctcc gcgcagttca ctccacggcg cttccgcgag cgctgcgggc ttgcgcagcg
      541 aagcgccgcc ggcgcgcgcg atcgcgtcgc ccttcgccgc gaacaccacc gtgttgctgc
      601 cgtcgcggtc ggtcacgcgc agcacccgct ggccgaagct gcgggcgatg cggtcgacgt
      661 acaccgcgtg ctgcggatga ccggcgtgca ggttgacgac gaacaggccg tctgtggcca
      721 gcgcgtcgag acagtcgtcg tagaagcgct gcgtgccgag cgcggcgggc atgccgtcgg
      781 catcgaaggc gtcgaccatc agcacgtcga agcgctcttc ggtggtcgcg atgagctgcg
      841 cgccgtcgcc ttcgacgacg cgaaagcgcg catcgtcggc cggcaccaga aaggcttcgc
      901 gcaacgcgat cacgcgcggg tcgatctcga cgacgagcat cgtggcgcgc ggcaggtgcc
      961 ggtggcagaa cttcgcgatc gaaccgccgc ccaggccgat catcgcgagc cgccgcgcct
     1021 gcggccggaa catcggccag cccatcatca gccgcgtgta ttccaactcc agcgcgtcgg
     1081 ggcggcgaag ctgcatgcgg ctctggatcg ccgatgtcga gaaatgcagc gaacgcgcgt
     1141 cgagttcctc gtgtacgtac ggcggcggcg tgtcggccat gcggacactt cagtcttcgg
     1201 actcgtccac gggccaggcg gccgcgtcgt cggcccagac ctgcagcgcc gcgccgcgcc
     1261 gcttcaactg cgcagcgagc ttgggcagcg cgtcgtcggg cagatgcgcc catgcgggcg
     1321 gcacgtcgcc gcggtcgagc gcctcgccgt gcagcaccca gcgcttgccg aacgcgcgca
     1381 gcgcgtcgcg cggcgtcggc agctcgtgcg cggcgatcgc gtcggtcggc aggtcgccgg
     1441 cgatcaccca ccagccgatc cattcgggcc gctgcgcgct ttccaccgcc cacaggctcg
     1501 cgtagggcat cgcgaaccac gccgggcgct cgccgatgcg gccatgcgcc ggcgcgagcc
     1561 gatccgcgca ggccgcgacc tcgacggcgc gcgcgtcgca ccagcgctct tcttcgcccg
     1621 cgtcggagct cgtcatctca agggcgccgc ggcggcaacg gaatgaactc ggtctcgccc
     1681 gggacacgct cgaaacgctg cgcctcccag tcgtcctgcg cctgcgcgat gcgctccttg
     1741 cggctcgaga cgaagttcca ggccatgaag cggtgcccga gcggcgtgcc gccgatcagc
     1801 acgatgtggc cgccgcgcgg cgcgctcagc agcggctcgc tgccgggctc gagcacgacc
     1861 atcgtgaacg cgggaacgag ttcgccgtcg agctcgaagt cgtggtcgac gctgtacagc
     1921 gcgcgctctt cggcagcgtc ggggatccgc agcacggcgc cggaggcgat ctcgaaattc
     1981 aggtccaggt agagcgtcgg cgacagggtg cgcaccggcg agctcagacc gaacgcgctg
     2041 ccgatcagta cgcgcaccag cacctgcgcg ttcgcgctgt cgacgtcacg cgccggaatt
     2101 tcggcgccgg gcacatgctg gaagccggga gcgtcttcct cgcgttcggt cggcaccgcc
     2161 agccacagct gcaggccgtg gctgcggcgc tcgatgccga gcaggtcctt cggcgtgcgc
     2221 tcggaatgca cgatgccgcg gccggccgtc atcaggttga tcgcgcccgg ctcgatgcgc
     2281 tgcaccacgc cggtcgagtc gcggtgcatc atcgcgccct cgaacaggta ggtcaccgtg
     2341 gccagaccga tgtgcgggtg cggccgcaca tcgaagttgt cgtcgggctt cgcggtgatc
     2401 gggccgaagt gatcgaagaa gacgaagggc ccgacggcct ggcgttgggc ggacggcagt
     2461 gaacggcgga cgatgaagcc gccgcccagg tccttctgat ggggctggag ggtttgaagc
     2521 tcgggcatgg ggcactccgg cagacagttc ggccagtgta ggt
//
LOCUS       DATMJB010000364         2164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_535270, whole genome shotgun sequence.
ACCESSION   DATMJB010000364 DATMJB010000000
VERSION     DATMJB010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535270"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..852)
                     /locus_tag="VNU48_07850"
     CDS             complement(82..852)
                     /locus_tag="VNU48_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HWI11226.1"
                     /translation="MIGDIGFGAAALLVLLLILLASALRILREYERAVVFQLGRFWKV
                     KGPGLVILIPGIQQMVRVGLRVVVMDIPSQDVISRDNVSVKVNAVLYFRVVDPEKAII
                     QVEHYLEATSQLAQTTLRAVLGKHDLDDMLSERERLNLDIQKILEAQTASWGIKVTNV
                     EIKHVDLNDTMIRAIARQAEAERERRAKVIHAEGELQASHKLAEAAQILAREPQALQL
                     RYLETLTVIAADKNSTIVFPLPMDILAPLLKGLPGRDS"
     gene            complement(849..2162)
                     /locus_tag="VNU48_07855"
     CDS             complement(849..2162)
                     /locus_tag="VNU48_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HWI11227.1"
                     /translation="MAASATVVPAAQAAPAPVAVMRVDGAIGAATADHLHRALARAAE
                     AHAQLAVLQLDTPGGLDVAMRSIIKDMLASPVPVAVFVAPNGARAASAGTYLLYASHV
                     AAMAPATNLGAATPVALGMPGLPAPSDAAPASAAASGAAASDTLTAKRISDAAAYLRS
                     LAQLRGRNADWAERAVRESVSLAAHEALRLGVIDLVAQDVPDLLRRLDGREIRLGERS
                     VRLATQGAPLLTIEADWRSRLLSVIADPSLALILMMIGIYGLLFEFANPGFVLPGVAG
                     AICLVLALFALQMLPVNYAGLAMILLGVGFFAAEAFVPSYGTLGLGGAVAFASGALLL
                     IDSDSPGFGVSPVLVGVLTAGSAAFVIFVAGMAAKARRRPLVSGPSALLGAAGELVEF
                     AAGEGWALVQGEHWKVHGGTALRPGDRVRVTRVRGALLEVEETTS"
BASE COUNT          331 a          774 c          756 g          303 t
ORIGIN      
        1 ggcagaaaat cgttctgaat gtcgaccacg agcagcacgt cgcggggggt aacgctaggt
       61 ctcatgatgg acgtacgcct tctaactgtc ccggccgggc agtcccttca agagcggcgc
      121 gagaatgtcc atcggaagcg ggaacacgat cgtggagttc ttgtcggcgg cgatcaccgt
      181 gagcgtctcg aggtagcgca gctgcagggc ctgcggttcg cgcgccagga tctgcgccgc
      241 ttcggcgagc ttgtgcgagg cctgcagttc gccctcggca tggatcacct tggcgcgccg
      301 ctcgcgctcg gcctcggcct ggcgtgcgat cgcgcggatc atcgtgtcgt tcaggtcgac
      361 gtgcttgatc tcgacgttcg tgaccttgat gccccagctc gcggtctggg cctcgaggat
      421 cttctggatg tccaggttca ggcgctcgcg ctccgacagc atgtcgtcca gatcatgttt
      481 gccgagcacc gcgcgcagcg tggtctgggc gagctggctg gtggcctcga ggtagtgttc
      541 gacctggatg atcgccttct ccgggtcgac gacgcggaag tacagcaccg cgttgacctt
      601 gaccgagacg ttgtcgcgcg agatcacgtc ctggctgggg atgtccatca cgaccacgcg
      661 cagcccgacc cgcaccatct gctggatgcc cgggatcagg atcaccaggc ccgggccctt
      721 gaccttccag aagcggccga gctggaacac gaccgcccgt tcgtactcgc gcaggatgcg
      781 cagcgcgctc gcgagcaaga tgagcagcag cacgagcagt gcggccgcgc cgaatccgat
      841 gtcgccgatc atgatgtcgt ctcctcgacc tcgagcagtg cgccgcgcac gcgcgtgacc
      901 cggacccggt cgccgggccg cagggcggtg ccgccgtgca ccttccagtg ctcgccctgc
      961 accagcgccc agccctcgcc ggctgcgaac tcgaccagtt caccggccgc accgagcagc
     1021 gcggacgggc cgctgaccag cgggcgccgg cgcgccttcg ccgccatgcc cgcgacgaag
     1081 atgacgaacg ccgccgagcc cgcggtcagc accccgacca gcaccggcga gacgccgaag
     1141 ccgggcgaat cgctgtcgat cagcagcagc gcacccgacg cgaacgcgac ggcgccgccc
     1201 aggccgagcg tgccgtagct cggcacgaag gcctcggcgg cgaagaagcc gacgcccagc
     1261 aggatcatcg ccagcccggc gtagttgacc ggcagcatct gcagcgcgaa cagcgcgagc
     1321 acgaggcaga tcgcgccggc cacgccgggc agcacgaagc ccgggttggc gaactcgaac
     1381 agcagcccgt agatgccgat catcatcagg atcagcgcca ggctcggatc ggcgatcacc
     1441 gacaacagcc ggctgcgcca gtcggcctcg atcgtgagca gcggcgcgcc ctgcgttgcc
     1501 agccgcacgc tgcgctcgcc caggcggatc tcgcgcccgt cgagccggcg cagcaggtcg
     1561 gggacatcct gcgcgacgag gtcgatcacg ccgagcctga gtgcttcgtg ggccgcaagg
     1621 ctgaccgatt cgcgcaccgc gcgctcggcc cagtcggcat tgcgtccgcg caactgcgcc
     1681 aggctgcgca ggtaggccgc ggcgtcggag atccgcttgg ccgtcagcgt gtcgctcgcg
     1741 gctgcgcccg acgctgcagc gctggcggga gcagcgtcgc tcggtgccgg caggccgggc
     1801 atgcccagcg cgacgggtgt cgccgcaccg agattggtgg ccggcgccat cgccgcgacg
     1861 tggctcgcat acagcaggta ggtgccggcg ctcgccgcgc gcgcgccgtt cggcgccacg
     1921 aacaccgcca ccggcaccgg tgaggccagc atgtccttga tgatgctgcg catcgcgaca
     1981 tcgagaccgc cgggcgtgtc gagctgcagc accgcgagct gggcatgcgc ctcggcggcg
     2041 cgcgccagcg cgcgatgcag gtggtcggcg gtggccgcgc cgatcgcgcc gtcgacgcgc
     2101 atcacggcca ccggcgccgg cgccgcctgc gcggccggca cgaccgtggc gctggccgcc
     2161 acgc
//
LOCUS       DATMJB010000365         3160 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_636908, whole genome shotgun sequence.
ACCESSION   DATMJB010000365 DATMJB010000000
VERSION     DATMJB010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636908"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(7..146)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            232..1443
                     /gene="mnmA"
                     /locus_tag="VNU48_07860"
     CDS             232..1443
                     /gene="mnmA"
                     /locus_tag="VNU48_07860"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWI11228.1"
                     /translation="MKPQRVVVGLSGGVDSAVSAHLLKRQGHEVVALFMKNWEDDDDD
                     EYCSSNIDFVDAAAVADVLGIEIEHVNFAADYKDRVFAEFLREYQAGRTPNPDVLCNA
                     EIKFKAFLDHALRLGAEKIATGHYARVRRVPASTSMAGGLDDESPAGAVDSARVRERS
                     GRFELLKGLDPLKDQSYFLHRLTQAQLGRTLFPVGELPKTEVRRIAAEIGLPNATKRD
                     STGICFIGERPFREFLNRYLANTPGPIMDDRGRTIGEHVGLSFYTLGQRKGIGIGGLK
                     ERGAPRAKAGTAAVRGGGAHAPWFVARKDMAHNTLYVVQGHDHPWLLSQRLHADDTSW
                     VAGHAPRPGAFAAKSRYRQQDADCTLAADTADAFELRFEQAQWAVTPGQSAVLYDGEV
                     CLGGGVIAGAA"
     gene            1489..2130
                     /locus_tag="VNU48_07865"
     CDS             1489..2130
                     /locus_tag="VNU48_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HWI11229.1"
                     /translation="MYLPAHFEESRPELLHALLRRHPLGLLVTQDAAGTLGADSVPFI
                     LDADPAGGPGILRAHVARANPVWREARGDVDALVVFQGPQAYISPAFYPSKAEHGKVV
                     PTWNYVTVQARGRLRAIDDADWLRAFVTRLTERHETPRAQPWAVSDAPAEYIATMLHA
                     IVGIEIVLTALTGKWKVSQNRSAADRAGVARGLGDMAFDFEACSAAPRASPAP"
     gene            2127..2264
                     /locus_tag="VNU48_07870"
     CDS             2127..2264
                     /locus_tag="VNU48_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11230.1"
                     /translation="MTRDSAALLGPRQLSLLSLWFMTRLPDLRLHDAPAAASGAAAAE
                     L"
     gene            2264..2962
                     /locus_tag="VNU48_07875"
     CDS             2264..2962
                     /locus_tag="VNU48_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWI11231.1"
                     /translation="MRRLVAAVLCAAAAFAQAAPKVEDSMAQRVQACTGCHGPQGRAA
                     SDGYYPRIAGKPAGYLYNQLVGFRDGRRRYALMAGLLAPLSDAYLHEIAEHFAALDLP
                     YPPPQPVTGSVDALARGEALVRRGDAALKLPACVQCHGAALMGVAPAVPALIGMPRDY
                     LNAQLGAWRTGSRHAPSPDCMAQVAQRLAPADIGALSQWLAAQPVPAGAKPAAGLPEP
                     MPLRCGGAPAEGAR"
     gene            2959..>3160
                     /locus_tag="VNU48_07880"
     CDS             2959..>3160
                     /locus_tag="VNU48_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWI11232.1"
                     /translation="MTRALVRTALALLTIALLLAALIWWLNTRDEEPLTSGGGAAASV
                     AADAEQIARGAYLARAGNCMGCH"
BASE COUNT          455 a         1164 c         1115 g          426 t
ORIGIN      
        1 agcaccctgc gccatcgctg aaccgtcggc cctggcatcg caccagatct gctgacctct
       61 tcatcgggag acgaagagcg ctcgcgggct cgcggcttgc gccgcctacc gccggtgggg
      121 aatttcgccc cgccctgaag acgtgtgccg cccatcgctg ggcagcgccc ggatcttacg
      181 accaagccct tggccgtgtg cgtcacaggg gcgacacgta aaatttgccc gatgaaaccc
      241 cagcgtgtcg tcgtcggcct gtcgggcggt gtcgactcgg ccgtcagcgc gcacctgctc
      301 aagcggcagg ggcacgaggt cgtcgccctc ttcatgaaga actgggaaga cgacgacgac
      361 gacgaatact gctcgtcgaa catcgacttc gtcgacgccg ccgcggtcgc cgacgtgctg
      421 ggcatcgaga tcgagcacgt caacttcgcc gccgactaca aagaccgcgt gttcgcggag
      481 ttcctgcgcg agtaccaggc cggccgcacg ccgaaccccg acgtgctgtg caacgccgag
      541 atcaagttca aggccttcct cgaccacgcg ctgcggctcg gggccgagaa gatcgcgacg
      601 ggccattacg ctcgtgttcg ccgtgtaccg gcgagcacga gcatggccgg tggcctcgat
      661 gacgaatcgc ccgctggcgc ggtcgatagc gcgcgggtgc gcgaacgcag tggccgcttc
      721 gaactgctga agggcctgga cccgctgaag gaccagagct acttcctgca ccgcctgacg
      781 caggcgcagc tcggcaggac gctgttcccg gtcggcgagc tgcccaagac cgaggtgcgc
      841 cgcatcgccg ccgagatcgg cctgccgaac gcgacgaagc gcgactcgac cggcatctgc
      901 ttcatcggcg aacggccgtt ccgcgagttc ctgaaccgct acctcgcgaa cacgccgggc
      961 ccgatcatgg acgaccgcgg ccgcacgatc ggcgagcacg tcggcctgag cttctacacg
     1021 ctcggccagc gcaagggcat cggcatcggc ggactgaagg aacgcggtgc gccgcgcgcg
     1081 aaagcgggaa ccgccgctgt tcgcggcggg ggcgcacacg cgccctggtt cgtcgcgcgc
     1141 aaggacatgg cgcacaacac gctctacgtg gtgcagggcc acgaccaccc ctggctgctg
     1201 tcgcagcggc tgcatgccga cgacaccagc tgggtcgccg gccacgcgcc gcggcccggt
     1261 gcgttcgcgg cgaagtcgcg ctaccgccag caggacgccg actgcacgct ggcagccgac
     1321 accgcagacg cattcgaact gcgcttcgag caggcccagt gggccgtcac gccgggccag
     1381 tcggcggtgc tgtacgacgg cgaggtctgc ctcggcggcg gcgtcatcgc cggcgcggcc
     1441 tgagcgagcg acgcgggcat gatgcgtgcc ccgacgcgtt gaacaatcat gtacctgccc
     1501 gcccacttcg aagagtcccg cccggagctg ctgcacgcgc tgctgcgccg gcatccgctg
     1561 ggcctgctgg tcacgcagga cgcggccggc acgctcggcg ccgacagcgt gcccttcatc
     1621 ctcgacgccg atccggccgg cggccccggc atcctgcgcg cccacgtcgc acgcgcgaac
     1681 ccggtgtggc gcgaggcgcg cggcgatgtc gatgcgctgg tcgtgttcca ggggccgcag
     1741 gcctacatct cgccggcctt ctacccgagc aaggccgagc acggcaaggt cgtgccgacg
     1801 tggaattacg tgacggtgca ggcgcgcggg cggctgcgcg cgatcgacga cgccgactgg
     1861 ctgcgcgcgt tcgtcacgcg cctgaccgaa cgccacgaga cgccgcgcgc gcagccctgg
     1921 gcggtgagtg acgcgccggc cgagtacatc gcaacgatgc tgcacgcgat cgtcggcatc
     1981 gagatcgtgc tgacggcgct gaccggcaag tggaaggtca gccagaaccg cagcgccgca
     2041 gaccgcgcgg gcgtcgcacg cggactcggc gacatggcct tcgactttga ggcttgttcg
     2101 gcggccccgc gcgcatcgcc tgccccgtga cccgagattc cgccgccctg ctcggcccgc
     2161 gccagctctc gctgctgtcg ctgtggttca tgacgcggct gccggacctg cggctgcacg
     2221 acgcgcccgc cgcggcgagc ggcgcggccg cggccgagct ctgatgcgca ggctcgtcgc
     2281 cgcagtgctg tgcgccgccg cggcgttcgc tcaggctgcg ccgaaggtcg aggactcgat
     2341 ggcgcagcgc gtgcaggcct gcaccggctg ccacggcccg cagggccgcg ccgcgagcga
     2401 cggctactac ccgcgcatcg ccggcaagcc ggccggctac ctctacaacc agctcgtcgg
     2461 ctttcgcgac gggcggcggc gctacgcgct gatggccggc ttgctcgcgc ccttgtcgga
     2521 cgcctacctg cacgagatcg ccgagcactt cgcggccctc gatctgccct acccgccgcc
     2581 gcagccggtg accggcagcg tcgacgcgct ggcgcgcggc gaggcgctgg tgcggcgcgg
     2641 cgacgcggcg ctgaagctgc cggcctgcgt gcagtgccac ggcgcggcgc tgatgggcgt
     2701 cgcgcccgcg gtgccggcgc tgatcggcat gccgcgcgac tacctgaacg cgcagctcgg
     2761 cgcctggcgc accggcagcc gccacgcgcc gtcgccagac tgcatggcgc aggtcgcgca
     2821 gcgcctcgcg ccggccgaca tcggcgcgct gtcgcaatgg ctcgccgcgc aacccgtgcc
     2881 cgccggtgcg aagccggcgg cgggcttgcc ggagccgatg ccactgcgct gtggtggcgc
     2941 gccggccgag ggcgcacgat gacgcgcgcg ttggtgcgca ccgcgctcgc gctgctgacg
     3001 atcgcactgc tgctcgccgc gctgatctgg tggctgaaca cgcgcgacga ggaaccgctg
     3061 acgagcggcg gcggcgccgc agcgagcgtc gcggccgatg ccgaacagat cgcgcgcggc
     3121 gcctacctcg cgcgcgccgg caactgcatg ggctgccaca
//
LOCUS       DATMJB010000366         7153 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_600063, whole genome shotgun sequence.
ACCESSION   DATMJB010000366 DATMJB010000000
VERSION     DATMJB010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7153
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600063"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..757
                     /locus_tag="VNU48_07885"
     CDS             62..757
                     /locus_tag="VNU48_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HWI11233.1"
                     /translation="MKRLTVLALAIAGIALAGCETMSETLNKRPPVDVLKTEAVRPPS
                     STVAPINNGAIFQAGQYRPLFEDHRARLVGDTITVQIVEKVSATQTSTSSIDKSGGLA
                     AGVTAIPGFAANSFTPARATIGATSSNTFAGKGSTQNSNDFAGTITATVIEVLPNGHL
                     IVSGEKQIGVNHNVDVLRFSGQIDPRAIQPGNTVASAQIANVRIEQRGKGAQADAQGI
                     GWLGRFFLNVLPI"
     gene            793..1926
                     /locus_tag="VNU48_07890"
     CDS             793..1926
                     /locus_tag="VNU48_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HWI11234.1"
                     /translation="MHDTDKLLRKLIAATALLASAALWWPAPAEAMRLKEVASVQGVR
                     SNQLVGYGLVVGLDGTGDQTTSTPFTTQSLNALLQQMGVTVPPGTNMQLKNVAAVMVT
                     AQLPAFAQPGQQIDVTVSSLGNAKSLRGGTLIATPLKGADGQIYALAQGNLIIGGAGA
                     SAAGSKVQVNTLNAGRVPEGATVERAVATPLNQGEYLQLDLNANDYNTAREVERAINN
                     KMGAGLAQALDGRVVRVRMPASTEARVAFMADIENLPLELAAPAAKVVINARTGSVVV
                     NQTVTLNACAVAHGSLSVTINSTPVISQPSPLSSGGTTVSREKADITIRQEPGSLIQL
                     PAGTKLADVVKALNSLGATPQDLLAILQAMKTAGALNAEIEVI"
     gene            1928..2830
                     /gene="flgJ"
                     /locus_tag="VNU48_07895"
     CDS             1928..2830
                     /gene="flgJ"
                     /locus_tag="VNU48_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HWI11235.1"
                     /translation="MAISGVSNTTGDAGALDALRDPSGSALRARAASDPHAAIKAAAK
                     QFEAMFMQQLMKSMRESSLGTGMLENSGTQMGTEMLDAQFANKMAGAPGGLADVIARQ
                     LERQISGTGAPAQALAPTSAAGAPSGGGAAQSVSPSQQEFLRLHQQSAAGAEAQSGIP
                     ASFMVAQAAHESGWGKHEIRNADGTSSHNLFGIKAGAGWTGQVAQVTTTEVVDGQPRK
                     VVAKFRAYASYDESFRDYAQMMKNSPRYAGVVAAGNSAQGFAQGLQRAGYATDPAYAD
                     KLTRVINTTLRLQRAALRTPPTLA"
     gene            2921..4861
                     /gene="flgK"
                     /locus_tag="VNU48_07900"
     CDS             2921..4861
                     /gene="flgK"
                     /locus_tag="VNU48_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HWI11236.1"
                     /translation="MSGLMSIGKTAMFANYAALQTVGNNIANANTPGYSRQETQLADA
                     PGQFTGSGFFGKGVTVTTIARSYDAYLTSQAVGTSATAAADSARLDKLTQLENVFPLG
                     DSGLGATAGQFLNSFVDLSNTPADSSARQVVLSQAQALASQFAAAGDQLSALQTGVTQ
                     DVKTSVASLNAFAQQVAQLNHQIAALQGSGQPPNQLLDQRDQLVRQIAGIVNVTTIPA
                     DDGSLGLFIGGGQSLVLGANANTVKAVPDAFDPAKVSLALSEGAVSRLIPPDSLAGGS
                     LSGLLKFQNEDLTAAKNLLGQMATAVSGAVNTQQSLGLDLSQPAGAGAPIFSVGTPRV
                     LPASTNTGSAALSLTVSDTSKVQPSDYAMSFDGTNYVLTRSSDQTAVAGSPFTPAQLA
                     AGVQFDGVTLQLTSGTPASNDRFVLQPVAQAAQTMQMVLGSPQGLAAAAPFTGSMGVS
                     NTGTATLASLVAVNPSYNGALSANIAFTSDTGDYSWTLSDGSAGTGTWTAGSLISLNG
                     FELSLAGVPKSGDTVDVKPTTAVASNNGNAVAFAKLADRGVVAAAGGANGSAGAQTIT
                     DAYASAIANVGVRVQSGKTASSISSAVASQAEAARSNKSGVNLDEEAAKLIQFQQSYQ
                     AAAKILQVAQQIFDTMLQTAAG"
     gene            4916..6112
                     /gene="flgL"
                     /locus_tag="VNU48_07905"
     CDS             4916..6112
                     /gene="flgL"
                     /locus_tag="VNU48_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HWI11237.1"
                     /translation="MRISTANNYAASIETLMDRQVKLSDTQEQMTSGKRVNRASDDPA
                     AAARAERAYAAEKRADASQRAVDASSNAMTLTEGALGDAGDLMQQVREALVAAGNATF
                     GDTERAGLANQIADLRQQLLSVANRTDGAGNYLFGGQSASVAPFSDAPGGVQFEGAPG
                     PTQSASADALPLTLDGSAAWMNARTGNGVFTTSPVTSTGSAWIDAGKVTDPSAITGST
                     YSIQFSVSGGNTTYSVLKDGVATAQANVAFQPGQAVQIDGMSASIAGQPANGDVFQLA
                     PSTPSMSVFDALDQAIADLKTPNRSNTQIAQSNAVNLNNVDVVMNQLGSVRSQVGATM
                     NRVDAVTSRLSAQKLSSQTERSNAEDLDMTQAISDFSNQQTGYDAALKAYATVQRLSL
                     FNYLNG"
     gene            6151..>7153
                     /locus_tag="VNU48_07910"
     CDS             6151..>7153
                     /locus_tag="VNU48_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWI11238.1"
                     /translation="MNDAAILGQVALGYSPLIDRKRAVTATRLTVFPLRPDAVLDVGQ
                     LLHEVGNVWPATGARVSLNIVSESLLQELLLARPSANLMVEVPSFMAADPANVEALCA
                     LHSNGGTLLLKGRPLTELPRAVLPCFKYSIIDLADDRRVTDPGAPAGVTRNIAHVQSG
                     VRTVADMEGSFARGAEAVLGWPIDDAVTTTAARSGKAVAAPGMQAIVELIHQVDKEES
                     IERLENTLKRDPALAFKLLRYINSPAFGLRVEISSFRHAIMMLGYKRLKRWLALLLAT
                     ASKDVNLKPVMFAAVRRGLLMEELSRSVADEEMRNEVFICGVFSLLDRMFQQPFSQLL
                     "
BASE COUNT         1223 a         2550 c         2430 g          950 t
ORIGIN      
        1 tcgcgcgtct gacgcagctg tgagctgcgt cctcaagccc cctgcagttg tgagcggtgc
       61 gatgaagcga ctgaccgtgc tcgcgctggc gatcgccggc atcgccctcg cgggctgcga
      121 aacgatgagc gagacgctga acaagcgccc gccggtcgac gtgctcaaga ccgaggcggt
      181 gcgcccgccg tccagcaccg tcgcgccgat caacaacggc gcgatcttcc aggccggcca
      241 gtaccggccg ctgttcgagg accaccgcgc gcgcctggtc ggcgacacga tcacggtgca
      301 gatcgtcgag aaggtcagcg cgacgcagac cagcaccagc tcgatcgaca agtccggcgg
      361 cctcgcggcc ggcgtcacgg cgatcccggg cttcgcggcg aactcgttca cgccggcacg
      421 cgcgaccatc ggcgcgacct cgagcaacac cttcgccggc aagggcagca cgcagaacag
      481 caacgacttc gccggcacga tcaccgccac ggtgatcgag gtgctgccca acggccacct
      541 gatcgtctcg ggcgagaagc agatcggcgt caaccacaac gtcgacgtgc tgcgcttctc
      601 gggccagatc gacccgcgcg cgatccagcc cggcaacacc gtggcctcgg cgcagatcgc
      661 gaacgtgcgc atcgagcagc gcggcaaggg cgcgcaggcc gatgcgcagg gaataggctg
      721 gttgggacgc ttctttttga acgttctgcc gatttaagcc cacccagaat gggtgcatcc
      781 gaggatgtac ccatgcacga caccgacaaa ctcctgcgca agctgatcgc tgccaccgcc
      841 ctgctggctt ccgccgcgct gtggtggccg gccccggccg aggcgatgcg cctgaaggag
      901 gtcgccagcg tgcagggcgt gcgcagcaac caattggtcg gctacggcct ggtcgtcggt
      961 ctcgacggca ccggcgacca gaccacctcg accccgttca cgacgcagag cctgaacgcg
     1021 ctgctgcagc agatgggcgt caccgtgccg ccgggcacca acatgcagct gaagaacgtc
     1081 gccgcggtga tggtcaccgc gcagctgccg gcgttcgcgc agccgggcca gcagatcgac
     1141 gtgacggtct cgtcgctggg caacgccaag tcgctgcgcg gcggcacgtt gatcgccacg
     1201 ccgctgaagg gcgccgacgg ccagatctac gcgctcgcgc agggcaacct gatcatcggc
     1261 ggcgcgggcg cctcggcggc cggatcgaag gtgcaggtca acacgctgaa tgccggccgc
     1321 gtgcccgaag gcgcgaccgt cgagcgcgcg gtggccacac cgctgaacca gggcgagtac
     1381 ctgcagctgg acctgaacgc gaacgactac aacaccgcgc gcgaggtcga gcgtgcgatc
     1441 aacaacaaga tgggcgcggg cctggcgcag gcgctcgacg gccgcgtcgt gcgcgtgcgc
     1501 atgccggcca gcaccgaggc gcgcgtggcc ttcatggccg acatcgagaa cctgccgctc
     1561 gagctcgccg cgccggcggc gaaggtcgtg atcaacgcgc gcaccggctc ggtcgtcgtg
     1621 aaccagacgg tcaccttgaa cgcctgcgcg gttgcgcacg gcagcctgtc ggtgacgatc
     1681 aactcgacgc cggtgatcag ccagccgtcg ccgctgtcca gcggcggcac gacggtgtcg
     1741 cgcgagaagg ccgacatcac gatccgtcag gagccgggct cgctgatcca gctgccggcc
     1801 ggcaccaagc tcgccgacgt cgtcaaggcg ctgaactcgc tcggcgcgac gccgcaggac
     1861 ctgctcgcga tcctgcaggc gatgaagacc gccggcgcgc tgaacgccga gatcgaggtg
     1921 atctgacgtg gcgatctcgg gcgtttcgaa caccaccggc gacgccggcg cgctcgatgc
     1981 gctgcgcgac ccgtcgggca gtgcgctgcg cgcgcgtgcc gcgagcgacc cgcacgccgc
     2041 gatcaaggcc gccgccaagc agttcgaggc gatgttcatg cagcagctga tgaagagcat
     2101 gcgcgaatcg tcgctgggca ccggcatgct cgagaactcg ggcacgcaga tgggcaccga
     2161 gatgctcgat gcccagttcg cgaacaagat ggccggtgcg ccgggcgggc tggccgacgt
     2221 gatcgcgcgc cagctcgaac gccagatcag cggcaccggt gcacccgcgc aggcgctcgc
     2281 cccgacgagc gcggcgggcg cgcccagcgg cggcggcgcg gcgcagagtg tcagcccgag
     2341 ccagcaggaa ttcctgcgcc tgcaccagca gtcggccgcc ggcgccgagg cgcagagcgg
     2401 catcccggcc agcttcatgg tcgcgcaggc ggcgcacgag tcgggctggg gcaagcacga
     2461 gatccgcaac gccgacggca cgagttcgca caacctgttc ggcatcaagg ccggtgcggg
     2521 ctggaccggt caggtcgcgc aggtcacgac caccgaggtc gtcgacggcc agccgcgcaa
     2581 ggtggtcgcg aagttccgcg cctatgcgag ctacgacgaa tcgtttcgcg actacgcgca
     2641 gatgatgaag aacagcccgc gctacgccgg cgtcgtcgcc gccggcaaca gcgcgcaggg
     2701 tttcgcgcaa ggcctgcagc gcgccggcta cgccaccgac ccggcctacg ccgacaagct
     2761 gacacgcgtc atcaacacca cgctgcgcct gcagcgcgcc gcgctgcgca cgccgccgac
     2821 gctggcatga aaggcaacat gaacaacccc tacgctcact tcgttcactg ccccccgagg
     2881 gggcgctcaa tgtccttcgg actgccgggc gggcattgag atgagcggat tgatgtccat
     2941 cggcaagacc gcgatgttcg cgaactacgc ggccttgcag acggtcggca acaacatcgc
     3001 gaacgcgaac acgcccggct actcgcgcca ggaaacgcag ctcgccgatg cgccgggcca
     3061 gttcaccggc tcgggcttct tcggcaaggg cgtgaccgtc acgacgatcg cgcgctcgta
     3121 cgatgcctac ctgaccagcc aggccgtggg caccagcgcc accgccgcgg ccgacagcgc
     3181 acggctggac aagctgacgc agctcgagaa cgtgttcccg ctcggtgact cggggctggg
     3241 cgcgaccgcc ggccagttcc tcaactcgtt cgtcgacctg tcgaacacgc cggcggattc
     3301 gtcggcgcgc caggtcgtgc tgagccaggc gcaggcactg gcgtcgcagt tcgccgcggc
     3361 cggcgaccag ctcagcgcac tgcagacggg cgtgacgcag gacgtgaaga cctcggtcgc
     3421 gtcgctcaac gcttttgcac agcaggtcgc gcagctgaac catcagatcg ccgcgctgca
     3481 gggctcgggc cagccgccga accagctgct ggaccagcgc gaccagctcg tccgccagat
     3541 cgccggcatc gtcaacgtca cgacgatccc ggccgacgac ggcagcctcg gcctgttcat
     3601 cggcggcggc cagagcctgg tgctcggcgc caacgcgaac accgtgaagg ccgtgcccga
     3661 tgccttcgat ccggccaagg tgtcgctggc gctcagcgag ggcgcggtca gccgcctgat
     3721 cccgccggac agcctggccg gcggttcctt gtcgggcctg ctgaagttcc agaacgagga
     3781 cctgaccgca gcgaagaacc tgctcggcca gatggccacg gcggtcagcg gcgcggtcaa
     3841 cacccagcag tcgctgggcc tggacctcag ccagccggcc ggtgccggcg cgccgatctt
     3901 ctcggtgggt acgccgcgcg tgctgccggc ctcgaccaac accggcagcg cggcactgag
     3961 cctgaccgtc agcgacacct cgaaggtgca gcccagcgac tacgcgatga gcttcgacgg
     4021 cacgaactac gtgctgaccc gctccagcga ccagaccgcc gtggccggca gcccgttcac
     4081 gccggcccag ctcgccgcgg gtgtgcagtt cgatggtgtc acgctccagc tcaccagcgg
     4141 cacgccggcg agcaacgacc gcttcgtgct gcagccggtg gcgcaggcgg cgcagaccat
     4201 gcagatggtg ctcggcagcc cccaggggct ggccgcggcg gcgccgttca ccggcagcat
     4261 gggcgtgagc aacaccggca ccgcgacgct ggcctcgttg gtggcggtca acccgtcgta
     4321 caacggcgcg ctgagcgcga acatcgcgtt cacgtccgac accggcgact acagctggac
     4381 gctcagcgat ggcagcgccg gcaccggcac ctggaccgcc ggctcgctga tcagcctgaa
     4441 cggcttcgag ctgagcctgg ccggcgtgcc caagagcggc gacacggtcg acgtgaagcc
     4501 gaccaccgcg gtcgcctcca acaacggcaa cgcggtcgcg ttcgcgaagc tggccgaccg
     4561 cggcgtcgtc gccgccgccg gcggtgcgaa cggcagcgcc ggcgcgcaga ccatcaccga
     4621 cgcctatgcg agcgcgatcg ccaacgtcgg cgtgcgcgtg cagagcggca agaccgcgtc
     4681 gagcatctcc agcgccgtcg cgagccaggc cgaggcggcg cgctcgaaca agtccggcgt
     4741 caacctcgac gaagaggcgg ccaagctgat ccagttccag cagagctatc aggccgcggc
     4801 caagatcctg caggtcgcgc agcagatctt cgacacgatg ctccagaccg ccgcgggctg
     4861 aaccgccgag ggcgggattc gccacgcttc atgaatttga ttcaaggtct ccaccatgcg
     4921 catcagcacc gccaacaact acgccgcctc gatcgagacc ctgatggacc ggcaggtcaa
     4981 gctcagcgac acgcaggagc agatgaccag cggcaagcgc gtcaaccgcg ccagcgacga
     5041 cccggccgcc gcggcccgcg ccgagcgtgc ctatgcggcc gagaagcgcg ccgacgccag
     5101 ccagcgcgcg gtcgatgcga gcagcaacgc gatgacgctg accgagggcg cgctcggcga
     5161 tgccggcgac ctgatgcagc aggtgcgcga agcgctggtg gcggccggca acgcgacctt
     5221 cggcgacacc gagcgcgccg gcctggcgaa ccagatcgcc gacctgcgtc agcagcttct
     5281 gtcggtggcg aaccgcaccg acggcgcggg caactacctg ttcggcggcc agagcgcatc
     5341 ggtcgcgccg ttttccgacg cgccgggcgg cgtgcagttc gagggcgccc cgggcccgac
     5401 ccaatcggcc tcggccgacg ccctgccgct gacgctggac ggcagcgccg cgtggatgaa
     5461 cgcgcgcacc ggcaacggcg tgttcacgac ctcgcctgtc accagcaccg gcagcgcctg
     5521 gatcgacgcc ggcaaggtca ccgatccgtc ggcgatcacc ggctcgacct acagcatcca
     5581 gttcagcgtc agcggcggca acaccaccta ttcggtgctg aaggacggcg tcgccacggc
     5641 ccaggccaac gtcgcgttcc agcccggcca ggcggtgcag atcgacggca tgtcggcgtc
     5701 gatcgcgggc cagccggcca atggcgacgt gttccagctc gcgccgtcca cgccgtcgat
     5761 gagcgtgttc gacgcgctcg accaggcgat cgccgacctg aagacgccga accgcagcaa
     5821 cacccagatc gcccagtcca acgccgtcaa cctgaacaac gtcgacgtcg tgatgaacca
     5881 gctcggttcg gtgcgcagcc aggtcggcgc gaccatgaac cgcgtcgacg cggtgacgag
     5941 ccgcctctcg gcgcagaagc tgtcgagcca gaccgagcgt tcgaacgccg aggatctgga
     6001 catgacgcag gcgatctccg atttttccaa ccagcagacc ggctacgatg cagccctgaa
     6061 ggcgtacgcg acggtgcagc gcctgtcgct gttcaactac ctcaatggct gaagtgacat
     6121 gactttcgac ccgactgcgg ggggtgttgc ttgaacgatg ccgccatcct cggccaggtc
     6181 gcgctgggct actcgcccct catcgaccgc aagcgcgccg tcacggcgac gcggctgacc
     6241 gtgttcccgc tgcggccgga cgcggtgctc gatgtcggcc agctgctgca cgaggtcggc
     6301 aacgtctggc ccgcgaccgg cgcgcgcgtg tcgctcaaca tcgtcagcga gagcctgctg
     6361 caggagctgc tgctggcgcg gccgtcggcg aacctgatgg tcgaggtgcc gtcgttcatg
     6421 gccgccgacc cggccaacgt cgaggcgctg tgcgcgctgc acagtaacgg cggcacgctg
     6481 ctgctgaagg gccggccgct gacggagctg ccgcgcgcgg tgctgccgtg cttcaagtat
     6541 tcgatcatcg acctggcgga cgatcgccgc gtcaccgacc cgggcgcgcc ggccggcgtg
     6601 acccgcaaca tcgcgcacgt gcagtcgggc gtgcgcaccg tcgccgacat ggaaggctcg
     6661 ttcgcgcgcg gcgccgaggc cgtgctcggc tggccgatcg acgatgcggt gacgaccacc
     6721 gcggcgcgca gcggcaaggc ggtcgccgcg cccggcatgc aggcgatcgt cgagctgatc
     6781 caccaggtcg acaaggaaga atcgatcgag cggctcgaga acacgctcaa gcgcgacccc
     6841 gcgctggcgt tcaagctgct gcgctacatc aactcgccgg cgtttggcct gcgcgtcgag
     6901 atcagctcgt tccgccacgc gatcatgatg ctgggctaca agcgcctgaa gcgctggctc
     6961 gcgctgctgc tggccaccgc gagcaaggac gtgaacctga agccggtgat gttcgccgcg
     7021 gtgcgccgcg gcctgctgat ggaagagctc tcgcgcagcg tcgccgacga ggagatgcgc
     7081 aacgaggtgt tcatctgcgg cgtgttctcg ctgctcgatc gcatgttcca gcagccgttt
     7141 tcgcagttgc tcg
//
LOCUS       DATMJB010000367         5865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_72210, whole genome shotgun sequence.
ACCESSION   DATMJB010000367 DATMJB010000000
VERSION     DATMJB010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72210"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="VNU48_07915"
     CDS             <1..1315
                     /locus_tag="VNU48_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11239.1"
                     /translation="TAIGLDTSGASLAAPSQTAVSYSIGGLPPGAFASLFQAIDGLRN
                     RRALAAMLGCMVVGVLVAGLFSMMAARLGFFVAFLGGLFLFVAAATGINAAGVLLMDQ
                     ARSVPSRPLVDAVVYGLMCIPKFIVLGLTLLLVAIAVFIALAIVYFVCRIPVLGPVLF
                     VAVFPLSVVIAGLTLCGLFLCMFLALPAIWEGDTITRAIAQTLAIARSRLVEAILLLA
                     VVGLLAGVVGFIVFSVLFTGLMPAIGLSASVLGGDGMYSVLGMMRHGSTDFGGVDAGG
                     GADAAAAGYAVAAGIGAGLLWALAGSLVSLVYLLGLNLVYLRITEGLDVGATEAAMKA
                     RFDEAKRQAADLGQKAREAAERAREQARQSAAAAQAGTTAAATKPAPPPEPPPAPPPT
                     IVSAGRELPPGSTTPAVAKTLTCPQCLSSVGQDDVFCGVCGYRLK"
     gene            complement(1329..2303)
                     /locus_tag="VNU48_07920"
     CDS             complement(1329..2303)
                     /locus_tag="VNU48_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HWI11240.1"
                     /translation="MSELHKFIFEGLPVRGMLVRLTDGWQEILARRAAAGDAFPAPVR
                     ALLGEMAAAGVLMQSNLRFNGALVLQMFGDGPVKLAVAEVQDDLAFRVTAKVIGEVGE
                     GARLEAMLNVHGQGRCAITLDPRDKRPGQQPYQGVVALHGDRREPLQQLAEVLEHYML
                     QSEQLDTRLILAANDEIAAGLLIQRLPVQGEANLQGVQRNEDLIGLDESFNRLASLAG
                     TLTRDELLTLDADTLLRRLFWQEPLRRFPPLRGDDGPRFVCSCSRARVGSMLKSLGRA
                     EIDGIVREQGRVEIGCDFCGLKYHFDPVDVGELFTPIGQQAPGSATTQ"
     gene            complement(2413..2937)
                     /locus_tag="VNU48_07925"
     CDS             complement(2413..2937)
                     /locus_tag="VNU48_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWI11241.1"
                     /translation="MALYQLDEHRPLLAEGAWVADSAQVIGRVELAEDASVWFGAILR
                     GDTELLRVGKGSNVQDGSVLHADPGYPLVLGDHVTIGHQVMLHGCSVGDGSLIGIGAV
                     VLNGAKIGRNCLVGAGALVTEGKEFPEGSMILGSPAKVVRQLTPEQIAALPRSAEYYV
                     LNAQRFKAGLKKIG"
     gene            3003..3791
                     /locus_tag="VNU48_07930"
     CDS             3003..3791
                     /locus_tag="VNU48_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWI11242.1"
                     /translation="MRPDARRQALAILTLGDPLEKAAQARTLYADLTDAAIDPDAQIT
                     ATGPLPGRPPLPRLVPAQSVPTRSPFTLEGRAALLHAVTHIEFNAINLALDAVWRFGH
                     MPRAFYRDWLRVASEEALHFTLLREHLLTLGHDYGAFDAHDGLWAMTDRTALDATARM
                     ALVPRTLEARGLDAAPPMQDKLRRAGDLHAVEILALILRDEVGHVAIGNHWYRWLCDR
                     DGLDPVAHYATLAQRYAAPRLRPPFNRAARLAAGFTEAEIALLQ"
     gene            3788..5302
                     /locus_tag="VNU48_07935"
     CDS             3788..5302
                     /locus_tag="VNU48_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006397257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HWI11243.1"
                     /translation="MTTTTLFVAGFHHETNTFAPSPADWAAFTSGAGYPGYARGDAML
                     AQMAPTSTALGGFVAEALEHGWALRPSVWAGAMPSNRVTADAFERICAEIVEDLQRGP
                     CDAVFLDLHGAAVAEGADDAEGELLARIRAVVGPDLPIVTALDLHANVTARMLALATA
                     MTAYRSYPHVDMRETGRRAARMLARRLVPAAPPRAVHAERVPFLLPLNAQCTLMQPAA
                     SVIALIGQIEAQHDVELNFAMGFAAADFAECGPVLFGYGIDPVRVRGAVQQLHTEVAV
                     TRRADWTVELLAPLAAVERAIALALEADAPVVIADTQDNPGAGGDANTTGMLHALLQA
                     RAGERLQGRVALGLLFDPESARAAVAAGVGATLPLTLGRAVPTWSGALTDAPVQALAR
                     VLAVSDGVVAMHGPMTAGATMRLGPSACVELQGIKLLLASAKAQMLDLDLYRYLGVEP
                     AQMKLLVNKSSVHFRAAFAPIASHILVAKAAGPMAADPGDLPWTKLPAGTATRP"
     gene            complement(5303..>5865)
                     /locus_tag="VNU48_07940"
     CDS             complement(5303..>5865)
                     /locus_tag="VNU48_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI11244.1"
                     /translation="LAIVQELAALMGGEVNVESEVGRGSHFWVDLPLKAAPNDALAPT
                     GLEANDADDDEAVSVLLVEDDLVNQMVVEEMLKMLGCEVEVVADGELAHRAAGAGVYD
                     IVFMDCHMPVMDGYEATRRIRAAEQGSGKRVPIVALTADSLATDRQRCLDAGMDGFMT
                     KPVSSAQLSAAIERWTGWRTNPATRW"
BASE COUNT          765 a         2177 c         2046 g          877 t
ORIGIN      
        1 gacggcgatc gggctcgaca cctcgggcgc gtcactggcg gcgccgtcgc agaccgccgt
       61 ctcctacagc atcggcgggc tgccgcccgg cgcgttcgcc agcctgttcc aggccatcga
      121 cgggctgcgc aaccggcggg cgctggccgc gatgctcggc tgcatggtgg tcggcgtgct
      181 cgtcgccggc ctgttctcga tgatggccgc gcgactgggc ttcttcgtcg cctttctcgg
      241 cggcctgttc ctgttcgtcg ccgccgccac cggcatcaac gccgccggcg tgctgctgat
      301 ggaccaggcg cgcagcgtgc cgtcgcggcc gctcgtcgac gccgtcgtct acggcctgat
      361 gtgcatcccg aaattcatcg tgctcggtct gacgctgctg ctggtggcga tcgcggtgtt
      421 catcgcgctg gcgatcgtct acttcgtctg caggatcccg gtgctcggcc ccgtcctgtt
      481 cgtcgccgtg ttcccgctgt cggtggtgat cgcgggcctg acgctgtgcg gcctgttcct
      541 gtgcatgttc ctcgcgctgc cggcaatctg ggagggcgac acgatcacgc gggcgatcgc
      601 acagacgctg gcgatcgcgc gcagccggct ggtcgaggcg atcctgctgc tcgcggtcgt
      661 cgggctgctc gcgggcgtgg tcggcttcat cgtgttcagc gtgctgttca ccgggctgat
      721 gccggcgatc ggcctgtcgg cctcggtgct cggcggcgac ggcatgtatt cggtgctggg
      781 gatgatgcgc cacggcagca ccgacttcgg cggggtggac gctggcggtg gcgccgacgc
      841 cgcggccgcg ggttacgcgg tggcggccgg catcggcgcc gggctgctgt gggcactggc
      901 gggttcgctg gtgtcgctgg tctacctgct gggcctgaac ctggtgtacc tgcgcatcac
      961 cgaggggctg gacgtcggcg cgaccgaggc ggcgatgaag gcccgcttcg acgaggcgaa
     1021 gcgccaggcc gccgacctgg gccagaaggc gcgcgaggcc gccgagcgcg cgcgcgaaca
     1081 ggcacggcag tcggccgccg ctgcgcaggc cggcacgaca gccgcggcga ccaagccagc
     1141 gccaccgccc gagccgccgc cggcaccgcc gccgaccatc gtctccgcag gtcgcgaact
     1201 gccgcccggc tcgaccacgc cggccgtcgc caagaccctg acctgcccgc agtgcctgtc
     1261 ctcggtcggt caggacgacg tgttctgcgg cgtctgcggc taccggctga agtagcgtcg
     1321 ggaggccgtc actgcgttgt cgccgaaccc ggcgcctgct ggccgatcgg cgtgaacagc
     1381 tcgccgacgt cgaccggatc gaagtggtac ttcagaccgc agaagtcgca gccgatctcg
     1441 acccggccct gctcgcgcac gatgccgtcg atctcggcgc ggcccaggct cttgagcatg
     1501 ctgccgacac gagcgcgcga gcagctgcag acgaagcgcg ggccgtcgtc gccgcgcagc
     1561 ggcgggaagc ggcgcagcgg ctcttgccag aacagacggc gcagcagcgt gtcggcgtcg
     1621 agcgtcagca gttcgtcgcg ggtcaaggtg ccggccagcg acgcgagccg gttgaaggac
     1681 tcgtccaggc cgatcaggtc ctcgttgcgc tgcacaccct gcaggttggc ctcgccctgc
     1741 accggcaggc gctggatcag caggcccgcg gcgatctcgt cgttggccgc gaggatcagg
     1801 cgcgtgtcga gctgctccga ctgcagcatg tagtgctcca gcacttcggc cagttgctgg
     1861 agcggctcgc gccggtcgcc gtgcagcgcg accacgccct ggtaaggctg ctggccgggg
     1921 cgcttgtccc tcggatcgag cgtgatcgca cagcggccct ggccgtgcac gttcagcatc
     1981 gcctccagcc gcgcgccctc gccgacctcg ccgatcacct tcgcggtgac gcgaaacgcg
     2041 agatcgtcct ggacctcggc gaccgccagc ttcaccgggc cgtcgccgaa catctgcagc
     2101 accagcgcgc cgttgaagcg caggttcgac tgcatcagca caccggccgc cgccatctcg
     2161 ccgagcagcg cgcgcaccgg tgcggggaag gcgtcgcccg ccgccgctcg gcgcgccagg
     2221 atttcctgcc agccgtcggt caggcgcacc agcatgccgc gcaccggcaa gccttcgaag
     2281 atgaacttgt gcagttcgct catgacggca ccccgctcgc cgcgtacggc gagcgaccct
     2341 ccgcgagggt ctcgcccgcc ttggggcggc ccggcgccgg gctcgcaaac gctgcaccca
     2401 tcagtgcctt ggtcatccga tcttcttcag cccggccttg aagcgctgcg cgttgagcac
     2461 gtagtactcg gcactgcgcg gcaacgcggc gatctgctcg ggcgtcagct ggcgcacgac
     2521 cttggccggc gacccgagga tcatcgagcc ctcgggaaac tccttgcctt cggtcaccag
     2581 cgcgccggcg cccaccaggc agttgcggcc gatcttcgcg ccattcagca ccaccgcgcc
     2641 gatgccgatc agcgagccgt cgccgaccga gcagccgtgc agcatcacct gatggccgat
     2701 ggtcacgtgg tcgcccagca ccaacgggta gccggggtcg gcgtgcagca cgctgccgtc
     2761 ctgcacgttc gagcccttgc cgacgcgcag cagctcggtg tcgccgcgca ggatcgcgcc
     2821 gaaccacacg ctcgcgtctt cggcgagctc gacgcggccg atgacctgcg cgctgtcggc
     2881 gacccaggcg ccttcggcca gcagcggccg gtgttcgtcg agttggtaca aggccatgcg
     2941 gttcaccgtg gtgcgattcg aagctcgcga ttttagggcg cgcgcgccta tcatcgacgc
     3001 tcatgagacc cgacgcgaga cggcaggcgc tggcgatcct caccctgggc gacccgctcg
     3061 agaaggcggc gcaggcccgc acgctgtatg ccgacctgac cgacgcagcg atcgaccccg
     3121 acgcacagat caccgcgacc gggccgctgc ccggccgccc gccgctgccg cgcctcgtgc
     3181 cggcccagag cgtgccgacg cgctcgccgt tcaccctcga agggcgcgcc gcgctgctgc
     3241 acgcggtcac gcacatcgag ttcaacgcga tcaacctcgc gctcgacgcg gtctggcgct
     3301 tcgggcacat gccgcgcgcg ttctatcgcg actggctgcg cgtcgcgagc gaggaggcgc
     3361 tgcacttcac gctgctgcgc gaacacctgc tcacgctcgg ccacgactac ggcgccttcg
     3421 acgcgcacga cggcctgtgg gcgatgaccg atcgcaccgc cctcgatgcg accgcgcgca
     3481 tggcactggt gccgcgcacg ctcgaggcgc gcggcctgga cgccgcgccg ccgatgcagg
     3541 acaagctgcg gcgcgccggc gacctgcacg cggtcgagat cctcgcgctg atcctgcgcg
     3601 acgaggtcgg ccatgtcgcg atcggcaacc attggtaccg atggctgtgc gaccgcgatg
     3661 ggctcgaccc ggtcgcgcac tacgccacgc tcgcgcagcg ctacgcggcg ccgcgcttgc
     3721 ggccgccctt caaccgcgcc gcgcgcctgg ccgccggctt caccgaggcc gagatcgccc
     3781 tgctccaatg accaccacga ccctcttcgt cgccggcttc caccacgaga ccaacacctt
     3841 cgcgccctcg cccgcggact gggccgcgtt cacctccggc gccggctatc cgggctatgc
     3901 gcgcggcgac gcgatgctgg cgcagatggc gccgaccagc accgcgctcg gtggcttcgt
     3961 ggccgaggcg ctcgagcacg gctgggcgct gcggccgtcg gtctgggccg gggcgatgcc
     4021 gtcgaaccgg gtcacggccg acgccttcga gcgcatctgc gccgagatcg tcgaggacct
     4081 gcagcgcggc ccctgcgacg cggtctttct cgacctgcac ggcgccgccg tggccgaagg
     4141 cgccgacgac gccgagggcg aactgctcgc gcgcatccgc gccgtcgtcg gccccgacct
     4201 gccgatcgtc accgccctgg acctgcacgc caacgtgacg gcgcgcatgc tggcgctggc
     4261 caccgcgatg accgcgtacc gcagctaccc gcacgtcgac atgcgcgaga ccggccggcg
     4321 cgccgcgcgc atgctggcgc gccgcctcgt gcccgcggcg ccgccgcgcg cggtgcatgc
     4381 cgagcgcgtg cccttcctgt tgccgctgaa cgcgcagtgc acgctgatgc agccggccgc
     4441 gtcggtgatc gcgctgatcg ggcagatcga ggcgcagcac gatgtcgagc tcaatttcgc
     4501 gatgggcttc gcggctgccg acttcgccga atgcgggccg gtgctgttcg gctacggcat
     4561 cgaccccgtg cgcgtgcgcg gcgcggtgca gcagctgcac accgaggtcg cggtgacacg
     4621 ccgcgccgac tggacggtcg agctgctggc gccgctggcc gcggtcgagc gcgcgatcgc
     4681 gctcgcgctc gaggccgacg cgccggtcgt gattgccgac acgcaggaca accccggcgc
     4741 cggcggcgac gccaacacca ccgggatgct gcacgcgctg ctgcaggcgc gcgccggcga
     4801 gcggctgcag gggcgcgtcg cgctcggcct gctgttcgat cccgaatcgg cacgcgccgc
     4861 ggtggccgcc ggcgtcggcg cgaccttgcc gctgacgctc ggccgcgcgg tgccgacctg
     4921 gagcggcgca ctgaccgatg cgccggtgca ggctttggca cgcgtgctcg cggtcagcga
     4981 cggcgtcgtc gcgatgcacg gcccgatgac cgccggcgcg acgatgcgcc tggggcccag
     5041 cgcctgcgtc gagctgcagg gcatcaagct gctgctggcc agcgccaagg cgcagatgct
     5101 cgacctcgac ctgtaccgct acctcggcgt cgagccggcg cagatgaagc tgctggtgaa
     5161 caagagctcg gtgcactttc gtgccgcgtt cgcgccgatc gcctcgcaca tcctggtggc
     5221 caaggcggcg gggccgatgg cggccgatcc gggcgatctg ccgtggacga agttgccggc
     5281 cggcacggcg acgcggccgt gatcaccaac gggtcgcggg gttggtccgc cacccggtcc
     5341 agcgctcgat ggcggcggac agttgcgcac tgctcaccgg cttcgtcatg aacccgtcca
     5401 tgccggcatc gaggcagcgc tgccggtcgg tcgccaacga gtcggcggtg agcgcgacga
     5461 tcggcacgcg cttgcccgag ccctgctcgg cggcgcggat gcggcgcgtg gcttcgtagc
     5521 cgtccatcac cggcatgtgg cagtccatga acacgatgtc gtagaccccg gcaccggccg
     5581 cgcgatgcgc gagctcgccg tcggccacca cctcgacctc gcagccgagc atcttcagca
     5641 tctcttcgac gaccatctgg ttgacgagat cgtcctcgac gagcagcacc gacaccgcct
     5701 cgtcgtcgtc ggcatcgttc gcctccaggc ctgtcggtgc gagcgcatcg ttcggggccg
     5761 ccttcagcgg cagatcgacc cagaagtgcg agccgcggcc gacctcgctc tcgacgttca
     5821 cctcgccgcc catcagcgcg gcgagttcct gcacgatcgc caggc
//
LOCUS       DATMJB010000368         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_179134, whole genome shotgun sequence.
ACCESSION   DATMJB010000368 DATMJB010000000
VERSION     DATMJB010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179134"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VNU48_07945"
     CDS             <1..555
                     /locus_tag="VNU48_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HWI11245.1"
                     /translation="GTPAMIELDHNALFFALKKEAFHSSILEMELAGKSEQVLLRDYQ
                     MHPFRPIVLHIDFQRVDATTKIVKKVPLHFVNEENSPAVKTDACIVNHVVTELRISCL
                     ASQLPEFLTIDLGELTKGQTLHVNDIKLPAGIKVMTQGKPNPVIVSVVAQAAEEEVAA
                     APVIVAPAAAEKSKAKKTEKQNKK"
     gene            646..1272
                     /gene="pth"
                     /locus_tag="VNU48_07950"
     CDS             646..1272
                     /gene="pth"
                     /locus_tag="VNU48_07950"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWI11246.1"
                     /translation="MIRLLVGLGNPGPEYAATRHNAGFWFIDAVARELRAHLAPERSY
                     FGLAARVNRPDGPVWLLEPMTFMNLSGKSVAALARFFKIAPGEILVAHDELDLLPGHV
                     KMKLGGSHAGHNGLKDIQAQLGSADFWRLRLGIGHPGVKAEVVDYVLRKPIAEQRDAI
                     ATSIERAVSALDLLLAGEMDHALMKVHAKPPRPKPPKPATPDASEEPP"
     gene            1269..1676
                     /locus_tag="VNU48_07955"
     CDS             1269..1676
                     /locus_tag="VNU48_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11247.1"
                     /translation="MKSFASALLLGLLSTLAGAQPVYRCGNAYSQSPCPAGGRIVDAT
                     DPRSAAQRAEARRVAADEQRLAAELRRDRLADENAAKPSGAASLSGPAVASHAATAGP
                     RLKKKRRSAAKSPAIEEVVVLDPRTVKRRGTGR"
     gene            complement(1680..1955)
                     /locus_tag="VNU48_07960"
     CDS             complement(1680..1955)
                     /locus_tag="VNU48_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HWI11248.1"
                     /translation="MALLITDECINCDVCEPECPNQAISMGVDFYQIDPAKCTECVGH
                     FDEPQCVQVCPVDCIPVNPEHVESQESLWAKYRRLQRSAGAPSSTGA"
     gene            complement(1965..2468)
                     /gene="coaD"
                     /locus_tag="VNU48_07965"
     CDS             complement(1965..2468)
                     /gene="coaD"
                     /locus_tag="VNU48_07965"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWI11249.1"
                     /translation="MTSVTKLTAVYPGTFDPMTLGHEDLMRRASRLFERLIVAIAAGH
                     HKRTMFTIAERLEIATELAAKYPNVEVMAFRGLLRDFVVENGGKVVVRGLRAVSDFEY
                     EFQMAGMNRQLMPDVETVFLTPGDRYQFVSGTFVREIATLGGDVSKFVSPSVLSRLHQ
                     RVKATEA"
     gene            complement(2521..>2742)
                     /locus_tag="VNU48_07970"
     CDS             complement(2521..>2742)
                     /locus_tag="VNU48_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HWI11250.1"
                     /translation="PASFELVFIDPPFDAPLVEPALALAARLVVPQGFVYVEAGAALQ
                     PAALPELGLTPHRQGRAGAVHFHLLRRAD"
BASE COUNT          495 a          943 c          917 g          387 t
ORIGIN      
        1 ggcacgccgg ccatgatcga gctcgatcac aacgccctgt tcttcgcgct gaagaaggag
       61 gccttccact cgtccatcct cgagatggag ctggcgggca agagcgagca ggtgctgctg
      121 cgtgactacc agatgcaccc gttccgcccg atcgtgctgc acatcgattt ccagcgcgtc
      181 gacgcgacca ccaagatcgt caagaaggtg ccgctgcact tcgtcaacga agagaactcg
      241 ccggccgtca agaccgatgc ctgcatcgtg aaccacgtgg tcaccgagct gcgcatctcg
      301 tgcctggcat cgcagctgcc cgagttcctg acgatcgacc tgggcgagct gaccaagggc
      361 cagaccctgc acgtcaacga catcaagctg cccgccggca tcaaggtgat gacgcagggc
      421 aagccgaacc cggtgatcgt gtcggtcgtg gcgcaggcgg ccgaggaaga ggtcgccgca
      481 gcgccggtca tcgtcgcgcc cgcggccgcc gagaagagca aggccaagaa gaccgagaag
      541 cagaacaaga agtagaagaa acaagaacaa cgcacccgca cgggtgatgc gaagccccac
      601 ttcggtgggg cttttttgtg cgcacccgac tcgataattg aagccatgat tcgactcctt
      661 gtcggcctgg gcaacccggg ccccgaatac gcagcgacgc ggcacaacgc cggcttctgg
      721 ttcatcgacg cggtcgcgcg cgaactgcgc gcccacctcg cgccggagcg cagctacttc
      781 ggtctggcgg cacgggtgaa ccggccagac ggcccggtct ggctgctcga accgatgacc
      841 ttcatgaacc tgtcgggcaa atcggtcgcg gcgctcgcgc gcttcttcaa gatcgcgccg
      901 ggcgagatcc tggtggcgca cgacgagctc gacctgctgc ccggccacgt caagatgaag
      961 ctcggcggca gccacgccgg ccacaacggc ctgaaggaca tccaggcgca actcggcagc
     1021 gccgacttct ggcgcctgcg gctgggcatc ggccaccccg gcgtgaaggc cgaggtcgtc
     1081 gactacgtgc tgcgcaagcc gatcgccgag cagcgcgacg cgatcgccac gagcatcgag
     1141 cgggccgtct ccgcgctcga cctgctgctc gccggcgaga tggaccatgc gttgatgaag
     1201 gtgcacgcga agccgccgcg gcccaagccg cccaagcctg ccacccccga tgcatcggag
     1261 gagccgccat gaagtccttc gcttctgcac tgctgctggg gctgctgagc acgctcgcgg
     1321 gcgcgcagcc ggtctaccgc tgcggcaacg cctattcgca gtcgccctgt cctgcgggcg
     1381 gccgaatcgt cgacgcgacc gacccgcgca gcgcggccca gcgcgccgag gcgcggcgcg
     1441 tggcggccga cgaacaacgc ctcgctgccg agctgcggcg cgaccggctc gccgacgaga
     1501 atgcggcgaa gccgtcgggg gccgcgagcc tgagcgggcc ggcggtcgcg agccatgccg
     1561 caacggccgg gccgcgcctg aagaagaagc ggcgcagcgc ggcgaagtcg cccgcgatcg
     1621 aagaagtcgt cgtgctcgac ccgaggaccg tcaagcggcg cggcaccggc cgctgagcgt
     1681 caggcgccgg tcgaagacgg cgcgccggcg gagcgctgca ggcgccggta cttggcccag
     1741 agcgactcct gcgactcgac atgctccgga ttgacgggaa tgcaatcgac cgggcagacc
     1801 tgcacacact gcggctcgtc gaaatggccg acgcattcgg tgcacttggc cggatcgatc
     1861 tgatagaagt ccacgcccat cgagatcgcc tggttcgggc actcgggctc gcagacatcg
     1921 cagttgatgc attcgtcggt aatcagcagc gccatgcgtg atcctcaggc ctccgtcgct
     1981 ttcacccgct gatgcagccg gctcagcacc gagggtgaca cgaacttgga gacatcaccg
     2041 cccaaggtgg caatctcgcg cacgaaagtg cccgagacga actggtagcg gtcgcccggc
     2101 gtcaggaaca ccgtctcgac gtccggcatc agctggcggt tcatgcccgc catctggaat
     2161 tcgtactcga agtcgctgac cgcgcgcagg ccgcgcacca ccaccttgcc gccgttctcg
     2221 acgacgaagt cgcgcagcaa cccgcgaaat gccatcacct cgacgttcgg gtacttggcc
     2281 gcgagctcgg tcgcgatctc cagccgctcg gcgatcgtga acatcgtgcg cttgtggtgg
     2341 ccggccgcga tcgcgacgat caggcgctcg aacaggcggc tggcgcggcg catcaggtct
     2401 tcgtgaccca gggtcatcgg atcgaaggtg cccgggtaga cggcggtgag tttggtcacg
     2461 gaggtcaagg cgcgctcccg gcgttgaatt cggccgcagt gtagtcgcga cggcccggtt
     2521 tcagtcggcg cgccgcaaca gatggaagtg gaccgccccg gcgcggccct ggcggtgcgg
     2581 cgtcagcccg agttcaggca gcgccgccgg ttgcagcgcg gcaccggcct cgacgtagac
     2641 gaagccctgc ggcacgacca gccgcgccgc cagcgccagc gccggctcga ccagcggcgc
     2701 atcgaacggc ggatcgatga agaccagctc gaacgatgcc gg
//
LOCUS       DATMJB010000369         4541 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_48902, whole genome shotgun sequence.
ACCESSION   DATMJB010000369 DATMJB010000000
VERSION     DATMJB010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48902"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..460
                     /locus_tag="VNU48_07975"
                     /pseudo
     CDS             77..460
                     /locus_tag="VNU48_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            complement(471..1250)
                     /locus_tag="VNU48_07980"
     CDS             complement(471..1250)
                     /locus_tag="VNU48_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWI11251.1"
                     /translation="MSAASRSHLLLIPSYNTGPTVYETVRSARAQWNPVWVVTDGSTD
                     GTPEGLRRMAAVDSGLTVLELPVNAGKGAAVLHGLQAAQAQGFTHALTMDADGQHPAA
                     LIPAFMQASMHEPGAMVLGRPVFDASAPLLRVRGRRVSNWWTNLETLGAGIDDSLYGF
                     RVYPVEDLIAVMRRQPWMRRFDFDTEAVVRLAWRGVRPLNMAAPVRYLTAAEGGVSHF
                     RYGRDNLLLTWMHARLMLEFVLRLPGLLLRRALRRPPFQRV"
     gene            complement(1247..1972)
                     /locus_tag="VNU48_07985"
     CDS             complement(1247..1972)
                     /locus_tag="VNU48_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWI11252.1"
                     /translation="MLRRLYQSALFYVLLLHLGAMSLTWNLVCVVLYPFLSRRQGVVV
                     GRAAISSVYRGFWSFAQWLGLMRIDYSALDVLDREGGLIIAANHPSMLDALLVIARVP
                     RGICIMRASLMRNPFLGAGARLARYIRNDPPRGMIRGCVANLKEGGQLVLFPEGTRTI
                     HAPINPFRPGLTLIAQMAQVPIQTVLIESESKYLSKGWPILRLPRFPVVIRLRLGRRF
                     APEADHQGLLKRIEAYFAEELSR"
     gene            complement(1988..2845)
                     /locus_tag="VNU48_07990"
     CDS             complement(1988..2845)
                     /locus_tag="VNU48_07990"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HWI11253.1"
                     /translation="MTTVLVLATCGAAWADTPVEPGPDVDTGLGRGVRRPLWELGLGV
                     AGLRLPDYRGADQSRNYLLPLPYVVYRGTWLKADRDGARALLFDTRRVKVDVSVAGST
                     PSRSNDNAARAGMPNLSGTFELGPNLNITLASSSKGAWKLDLRLPLRAAATIERSPKF
                     IGTTFSPNLNLDVAGLGGGWNLGMLAGPVFADRKYHAYYYGVDAAYATADRPAYQAHG
                     GYAGWRALAATSRRFGNAWVGAFLRYDDLHGASFDDSPLLRRSSGLTFGLGVSWILAT
                     SSELVTASD"
     gene            complement(2863..3873)
                     /locus_tag="VNU48_07995"
     CDS             complement(2863..3873)
                     /locus_tag="VNU48_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11254.1"
                     /translation="MSVASSSTSRALAAPGPLVFRRLRLPHGAAPPASLGGRWVGAGA
                     LHAALLAPEGAVFDAWMVRAPTEERQHGCVRYATDGDWLHGIAEIDERAIGMQSAAHR
                     AYTDLFEVLAASGCGNLLRLWNYLADINLVVDGSERYRQFNVGRQQAFIEARRSAFEG
                     APTACALGVRDGPLRVYFLAGRRPPLAIENPRQVSAYRYPDAYGPRSPTFSRAALAEV
                     GGGRQVLFISGTASIVGHASVHIGDVPRQTEESLRNIAAVCQVAAGRAGVEIALGELV
                     CTVYVRRAADLAVVREVFESAVGADSPAARDCIYLQADICRAELLVEIEAHAVIPAGA
                     AA"
     gene            complement(3870..>4541)
                     /locus_tag="VNU48_08000"
     CDS             complement(3870..>4541)
                     /locus_tag="VNU48_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HWI11255.1"
                     /translation="HGWFWFIPLADGTTSVGAVCWPYYLKSRNKRPLPEFFRDTIALC
                     PELEKRLAGAVLVDDAVYATGNYAYSSTHCSGARYLMLGDAYAFIDPVFSSGVYLAMQ
                     SGFEGADAVETALDRPRAYAGARRRFDKMMRHGPREFSWFIFRVTNPTIREFFMDPRN
                     PLRVKEALLSLLAGDIYGKTPIWGSIRIMKALYYLVSIGNLRRTLRAWRRRRINIRDV
                     EGSAA"
BASE COUNT          749 a         1554 c         1622 g          616 t
ORIGIN      
        1 gaccactcat ttaaatagtt aatgacttac aaaaaaaaga aaaattaaat aaaaaacttc
       61 ctaaaataac tacctgggct acggcatcca ggccaacatc gactggaaga actcgctcgg
      121 caacgagtgg cgccagtacg agaagttctt tccggcactc gcggccagca gcatccagca
      181 ggtcagcctg gagttcatcc actcgcgcgt gccgcccgag ctgatgcggc tgctcgacgg
      241 caaggacgtg atggtcggcg tgatcgatgt ggccagcgat gtgatcgaga cgcccgaaga
      301 ggttgccgac acgattggcc gagccctgca gttcgtgccg cgcgatcggc tgcggccttg
      361 caccaactgc ggcctggcgc cgctgtcgcg cgcggtggcg ctcgccaagc tcgaggcgct
      421 ggggcgcggc gtggcgctgg cgcgcgaacg cctgggctga cgcggcgcgg ctagacgcgc
      481 tggaacggcg gccggcgcaa cgcgcgccgc agcagcaggc ccggcaggcg cagcacgaac
      541 tccagcatca gccgcgcgtg catccaggtc agcagcaggt tgtcgcggcc gtagcggaag
      601 tgcgagacgc cgccctccgc ggccgtcagg tagcgcaccg gcgccgccat attcagcggc
      661 ctgacgccgc gccacgcgag gcgcacgacg gcttcggtgt cgaagtcgaa gcggcgcatc
      721 cagggctggc ggcgcatcac cgcgatcagg tcttcgaccg gatagacgcg aaagccgtac
      781 agcgagtcgt cgataccggc gcccagcgtc tcgaggttgg tccaccagtt cgagacgcgc
      841 cggccgcgca cccgcagcag cggcgccgaa gcatcgaaca ccgggcggcc cagcaccatc
      901 gcgccgggct cgtgcatcga cgcctgcatg aacgccggga tcaatgcggc ggggtgctgg
      961 ccgtcggcgt ccatcgtcag cgcgtgcgtg aagccttgtg cctgcgcggc ctgcaagccg
     1021 tgcagcaccg cggcaccctt gccggcgttg acgggcagtt cgagcacggt caggcccgag
     1081 tccacggcgg ccatgcggcg cagcccttcg ggcgtgccgt cggtgctgcc gtcggtgacg
     1141 acccacaccg ggttccactg cgcgcgcgcg gagcgcacgg tctcgtagac cgttggcccg
     1201 gtgttgtagc tggggatcag cagcaggtgg ctgcgcgacg cggcactcat cggctcaatt
     1261 cttccgcgaa atacgcctcg atccgcttca gcaacccctg atgatcggcc tcgggggcga
     1321 agcgccggcc gagccgcagc cggatcacga cgggaaaccg cggcagccga aggatcggcc
     1381 agcccttgct gaggtacttc gattcggact cgatcagcac ggtctggatc ggcacctgcg
     1441 ccatctgcgc aatcagcgtc aggccgggcc ggaacgggtt gatcggcgcg tggatcgtgc
     1501 gcgtgccctc ggggaacagc acgagctggc cgccctcctt caggttcgcg acgcagccgc
     1561 ggatcatgcc gcgcggcggg tcgttgcgga tgtagcgtgc caggcgggcg ccggcgccga
     1621 gaaaggggtt gcgcatcagg ctcgcgcgca tgatgcagat gccgcgcggc acgcgcgcga
     1681 tcaccagcag cgcgtcgagc atgctggggt ggttcgcggc gatgatcagg ccgccctcgc
     1741 ggtccagcac gtcgagcgcg ctgtagtcga tgcgcatcaa ccccagccat tgcgcgaacg
     1801 accagaagcc gcggtagacc gacgagatcg ccgcgcgccc gaccaccacg ccctgccggc
     1861 gcgacaggaa cgggtacagc accacgcaga ccaggttcca ggtcagcgac atcgccccga
     1921 ggtgcagcag cagcacgtag aacagtgcgc tctggtacag acgcctgagc aaactcaaac
     1981 ctcggtctca gtccgaagcc gtcacgagct cggacgaggt cgccaggatc cacgagacgc
     2041 cgaggccgaa ggtcagcccc gagctgcggc gcagcagcgg gctgtcgtcg aagctcgcgc
     2101 cgtgcaggtc gtcgtagcgc aggaacgcgc cgacccaggc gttgccgaag cggcgcgagg
     2161 tcgcggccag cgcgcgccag ccggcgtagc cgccatgcgc ctggtaggcc gggcggtcgg
     2221 cggtcgcata ggccgcatcg acgccgtagt agtacgcgtg gtacttgcga tcggcgaaca
     2281 ccggccccgc gagcatgccg agattccagc cgccgccgag gccggcgaca tcgagattca
     2341 ggttcggcga gaaggtcgtg ccgatgaact tgggcgagcg ttcgatcgtc gccgcggcac
     2401 gcagcggcaa gcgcagatcg agcttccagg cacccttcga tgaactggcg agcgtgatgt
     2461 tcaggttcgg cccgagctcg aacgtgcccg acaggttggg catgccggcg cgcgccgcgt
     2521 tgtcgttgct gcggctcggc gtcgagccgg cgacgctcac gtcgaccttg acgcgccggg
     2581 tatcgaacag cagcgcgcgc gcgccgtcgc gatccgcctt cagccaggtg ccgcggtaga
     2641 cgacgtaggg cagtggcagc aggtagttgc ggctctggtc ggcgccgcgg tagtcgggca
     2701 acctcagccc ggccacgccg aggccgagtt cccacagcgg gcgccgcaca ccgcggccga
     2761 ggccggtgtc gacatcgggg ccgggctcga ccggcgtgtc ggcccacgcg gcgccgcatg
     2821 tcgcgagcac gagcacggtc gtcacagcgc gcagccgcgc cgtcacgccg ccgccccggc
     2881 gggaatgacg gcgtgcgcct cgatctcgac cagcagctcg gcgcggcaga tgtcggcctg
     2941 caggtagatg caatcgcgcg ccgcggggct gtcggcgccg accgcgctct cgaagacctc
     3001 gcgcacgacg gcgagatcgg cggcgcggcg aacgtagacc gtgcagacca gctcgccgag
     3061 cgcaatctcg acgccggcgc gtccggccgc gacctggcac accgcggcga tgttgcgcag
     3121 gctctcctcg gtctggcgcg gcacgtcgcc gatgtgcacg ctcgcgtggc cgacgatgct
     3181 cgcggtgcct gagatgaaca gcacctggcg gccgccgccg acctcggcga gcgcggcgcg
     3241 cgagaaggtc gggctgcgcg ggccgtaggc gtcggggtag cgataggcgc tgacctggcg
     3301 cgggttctcg atcgccagcg gcgggcggcg accggccagg aagtacacgc gcagcgggcc
     3361 gtcgcgcacg ccgagcgcac aggccgtcgg cgcgccctcg aaggcgctgc ggcgcgcttc
     3421 gatgaaggcc tgctggcggc cgacattgaa ctggcggtag cgctcgctgc cgtcgaccac
     3481 gaggttgatg tcggcgaggt agttccacag gcgcagcaga ttgccgcagc cgctcgcggc
     3541 cagcacctcg aacaggtcgg tgtaggcgcg gtgcgccgcg ctctgcatcc cgatcgcgcg
     3601 ctcgtcgatc tcggcgatgc cgtgcagcca gtcgccatcg gtcgcgtagc gcacgcagcc
     3661 atgctggcgt tcttcggtcg gggcgcggac catccaggca tcgaacaccg ccccttcggg
     3721 ggcgagcaac gccgcgtgca aggcgcccgc acccacccag cgcccgccga gcgaggccgg
     3781 tggtgcagcg ccgtgcggca gccgcaggcg gcggaagacc agcggaccgg gagcggcaag
     3841 cgcgcgcgac gtcgacgacg acgcgacact cacgcggccg agccctcgac gtcgcgaatg
     3901 ttgatgcgcc gccgccgcca cgcgcgcagc gtgcgccgca ggttgccgat cgagaccagg
     3961 tagtacagcg ccttcatgat gcggatcgag ccccagatcg gcgtcttgcc gtagatgtcg
     4021 ccggccagca gcgacagcag cgcctccttc acgcgcagcg ggttgcgcgg gtccatgaag
     4081 aactcgcgta tcgtcgggtt cgtgacgcgg aagatgaacc acgagaactc gcgcgggcca
     4141 tggcgcatca tcttgtcgaa gcggcgtcgc gcacccgcat acgcgcgcgg ccggtcgagc
     4201 gcggtctcga ccgcatcggc gccctcgaag ccgctttgca tcgcgaggta gacgccgctg
     4261 gagaacaccg ggtcgatgaa cgcatacgcg tcgccgagca tcaggtagcg cgcgccgctg
     4321 cagtgcgtgc tgctgtaggc gtagttgccg gtcgcgtaga ccgcgtcgtc gaccagcacc
     4381 gcgccggcca ggcgcttctc gagttcgggg cacagcgcga tcgtgtcgcg aaagaattcg
     4441 ggcagcggcc gcttgttgcg cgacttcagg tagtagggcc agcagaccgc gccgacgctg
     4501 gtcgtgccgt cggccagcgg gatgaaccag aaccagccgt g
//
LOCUS       DATMJB010000370         2372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_476730, whole genome shotgun sequence.
ACCESSION   DATMJB010000370 DATMJB010000000
VERSION     DATMJB010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476730"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VNU48_08005"
     CDS             complement(<1..736)
                     /locus_tag="VNU48_08005"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWI11256.1"
                     /translation="MFESTIAGSLPKPAWLAETEKLWPKWRAQGAELQQAKLDATLLW
                     IKAQEDAGLDVIGDGEQSRQHFVHGFLEQVEGIDFEHKVEMGIRDDRYKAMVPQVVAP
                     LKLKGRVHASEARFLRAHTTKKIKFTLPGPMTIVDTVADRFYGDRVTMAFAFAELLNQ
                     EALALQADGVDIIQFDEPAFNVYMQDAADWGIEALERAARGLACKTAVHICYGYGIKA
                     NVDWKQSLGAEWRQYEQVFPALAKSSI"
     gene            complement(837..1307)
                     /locus_tag="VNU48_08010"
     CDS             complement(837..1307)
                     /locus_tag="VNU48_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HWI11257.1"
                     /translation="MSRAHAVHVRHAAHRLVRWQRLVLYGSGAALLVSGAAWLAIHYT
                     IGAGAGELPHPLEAWCLRVHGLASFAALFVLGAIAAAHIPQGWRLTGRRRWSGQRRSG
                     LLLCTGSALLALTGYLLYYFAPESLRPALGWVHAGIGAAVLLLGIGHRRSHGAA"
     gene            complement(1312..2073)
                     /locus_tag="VNU48_08015"
     CDS             complement(1312..2073)
                     /locus_tag="VNU48_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HWI11258.1"
                     /translation="MSHAPLAGAWQRRARPLLGTLVEIGVAADDDTCGHATLEAAFAV
                     VEQVQACLSRFAADSDVARFHALRRGDSLTLRRAALDVLTAACELQRASHGAFDISLG
                     SAPLGWSVEGDRLIKLSDDVRLDLGGIGKGYAVDCAVEALIAQGVRSGWVNAGGDLRA
                     FGAAELSIQLRDERAGGVRPFASLHDAAFATSRFDRRSRSRLARGNGAPGASAHVSVA
                     ATRCLWADALTKIVAVSGDTAHPLLARYGASAWQH"
     gene            complement(2070..>2372)
                     /locus_tag="VNU48_08020"
     CDS             complement(2070..>2372)
                     /locus_tag="VNU48_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HWI11259.1"
                     /translation="FVVTDAVIGKVELIDYAVGIALDGSVKQIEILSYRESHGFEIRL
                     PAWRKQFVGKGAGAAIKVGDDIANISGATLSCTHVTDGVRRIVAVVALARRAGALT"
BASE COUNT          344 a          860 c          822 g          346 t
ORIGIN      
        1 cgatcgagct cttcgcgagc gcaggaaaga cctgttcgta ctggcgccac tcggcgccga
       61 gcgactgctt ccagtcgacg ttcgccttga tgccgtagcc gtagcagatg tgcaccgccg
      121 tcttgcaggc gaggccgcgc gcggcccgct cgagtgcctc gatgccccag tcggccgcgt
      181 cctgcatgta gacgttgaag gccggctcgt cgaactggat gatgtcgacg ccgtcggcct
      241 gtagcgccag cgcttcctgg ttcagcagtt cggcgaatgc gaacgccatc gtgacgcggt
      301 cgccgtagaa gcggtcggcc acggtgtcga cgatggtcat cgggccgggc agggtgaact
      361 tgatcttctt cgtggtgtgc gcgcgcagga agcgcgcttc gctcgcgtgc acccggccct
      421 tcagcttcag cggcgcgacc acctgcggca ccatcgcctt gtagcggtcg tcgcggatgc
      481 ccatctcgac cttgtgctcg aagtcgatgc cttcgacctg ttcgagaaag ccgtggacga
      541 aatgctggcg gctctgctcg ccgtcgccga tgacatccag ccccgcgtct tcctgggcct
      601 tgatccagag cagtgtggcg tcgagcttgg cctgctgcag ctcggcaccc tgcgcccgcc
      661 acttcggcca gagcttctcg gtctcggcca gccaggccgg cttgggcagg ctgccagcga
      721 tggtggattc gaacatggcg tgcgctttat cggtcggtgc cgcgcgagca taaccaatcg
      781 ccgggccgca ctcgtcgagt ccgccggcgt gtcgagacac ctcgtctcag ggccgatcag
      841 gccgcgccat gagagcgccg gtggccgatg ccgagcagca gcaccgcggc accgatgccg
      901 gcgtgcaccc agcccagcgc cgggcgcagg ctctcggggg caaagtagta cagcaggtag
      961 ccggtcagcg cgagcaacgc gctgccggtg cacagcagca gcccggagcg gcgctggccg
     1021 ctccatcgac ggcggccggt caatcgccag ccctgcggga tgtgcgccgc ggcgatcgcg
     1081 cccagcacga acagcgccgc gaacgacgcc aggccgtgca cgcgcaggca ccaggcctcg
     1141 agcggatgcg gcagttcgcc cgcgcccgcg ccgatggtgt agtggatcgc cagccatgcc
     1201 gcgccgctga cgagcagcgc cgcaccgctg ccgtacagca ccaggcgctg ccagcggacg
     1261 aggcggtgcg cagcgtggcg cacatgcacg gcgtgggctc ggctcatcgc atcagtgctg
     1321 ccaggcgctg gcgccgtaac gagccagcaa cgggtgcgcg gtgtcgccgc tgacggccac
     1381 gatcttggtc aacgcgtcgg cccacaggca gcgcgtagcg gcgacgctga catgcgcgct
     1441 tgcccccgga gcgccgttgc cccgcgcgag ccgcgaccgg ctgcgccggt cgaagcggct
     1501 ggtcgcgaac gcggcatcgt gcaaggacgc gaacggccgc acgccgccgg cgcgctcgtc
     1561 gcgcagctgg atcgacaact cggcggcgcc gaacgcgcgc aggtcgccgc cggcattcac
     1621 ccagccgctg cgcacgccct gcgcgatcag cgcctccacc gcgcagtcca cggcatagcc
     1681 cttgccgatg ccgccgaggt cgaggcgcac gtcgtcgctc agcttgatca ggcgatcccc
     1741 ttcgacggac cagccgagcg gcgcgctgcc caggctgatg tcgaaggcgc cgtgactcgc
     1801 gcgttgcagc tcgcacgcgg ccgtcagcac gtccagcgcc gcgcgccgca gcgtcagcga
     1861 gtcgccgcgc cgcagcgcat gaaagcgggc gacgtcgctg tccgcggcga agcgcgacag
     1921 gcaggcctgc acctgctcga cgacggcgaa cgccgcttcc agcgtggcat gcccgcaggt
     1981 gtcgtcgtcg gcggcgacgc cgatctcgac cagcgtgccg agcaagggcc gtgcgcgccg
     2041 ctgccaggct ccggccagcg gcgcgtggct cacgtcagcg ctccggcccg gcgcgcgagc
     2101 gcgaccaccg cgacgatgcg gcgcacgccg tcggtcacgt gggtgcagct cagcgtcgcg
     2161 ccgctgatgt tcgcgatgtc gtcgccgacc ttgatcgccg cgcccgcgcc cttgccgacg
     2221 aactgcttgc gccacgccgg cagccggatc tcgaagccgt ggctttcgcg gtacgagagg
     2281 atctcgatct gcttgacgct gccgtccagc gcgatgccga ccgcgtagtc gatcagctcg
     2341 actttgccga tcaccgcgtc ggtcacgacg aa
//
LOCUS       DATMJB010000371         3694 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_237361, whole genome shotgun sequence.
ACCESSION   DATMJB010000371 DATMJB010000000
VERSION     DATMJB010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237361"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VNU48_08025"
     CDS             complement(<1..1225)
                     /locus_tag="VNU48_08025"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HWI11260.1"
                     /translation="MLPQRRLAFLTNRRVRQRWQVRAVLWAAAGAAGLAVVGFATLAE
                     HALMLFDTIHTRWPWAPFVLAPSIGMLVVWATQRYVPGAAGSGIPQVVAATRLAALGR
                     SVQALLSLRVASAKVLLGSLALVGGFSAGREGPSVQVAASVLHLANRFLPHGRALRSS
                     DLILAGGAAGIAAAFNTPLAGIVFAVEELGRRLESRTSGVLVSTIIISGLVAIALKGN
                     YAYFGQLTVRDLGTAVLLPTLIAGLVCGVLGGLFSWLLLWPQRHPMGRLWTWRRAHPV
                     AFAGLCGLVVAALGWVSGGTSFGSGYAAASQAIGGQLTLEWHTPITRFAATIATYFSG
                     MPGGIFAPSLAVGTAVGSDLAQWVGAGVDPHAWIALCMAAFLAAVTQSPITAAIIVME
                     MVNGHQMVISLMAVAF"
     gene            complement(1261..1791)
                     /gene="pal"
                     /locus_tag="VNU48_08030"
     CDS             complement(1261..1791)
                     /gene="pal"
                     /locus_tag="VNU48_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HWI11261.1"
                     /translation="MTIKSLSIALIAAAALAACSSTPIDTKAPVASASTAPPATPPAA
                     STAQSSVATVTLDPLDDPKSQLAARSVYFAYDDFTVDPKYQPLVQAHGQFLQTHAAVQ
                     IRVEGNADERGSREYNLALGDKRAQIVAKQLELMGAKPAQIEAVSYGEERPKAEGHDE
                     AAWAENRRADIRYVKR"
     gene            complement(1833..2336)
                     /locus_tag="VNU48_08035"
     CDS             complement(1833..2336)
                     /locus_tag="VNU48_08035"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWI11262.1"
                     /translation="MKSDVTAPRRRRGAPGGLDVLQQLRVVLRLAGSHSARLERSTGI
                     PGAQLWALHEVAQADGLSVGELAQRLRMHQTTVSNLLTRLDAAGLVRKGRSPQDQRIV
                     QVHLTAAGRRGLKRAPGPARGLLPNVLERMSAAQLRKVHDGLAVLVDCMGGLDVARGA
                     KPLPFTE"
     gene            2463..3641
                     /locus_tag="VNU48_08040"
     CDS             2463..3641
                     /locus_tag="VNU48_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWI11263.1"
                     /translation="MNTELLGPLVAWPLMLLAAWSAGEWVFRHWNLPRVAAYGAIGLL
                     LGGVGLSQNVASQAAQGFMANAALALTLFELGYRINPRWFRHNPWVLAAGLVQALLTF
                     GAVFWVAGAFDVPLDHRLVLAALCVATSPAAVMRVTHELRSAGQVTERLLHLCALNCL
                     VAVLLLKLVVGYWHLETSGDLGKAAFNSLYVIGLSLVAGATFGVIAPFLNRIATSSQS
                     TTVTFAFLVLLLTGIAQTLHLSPLLAALAFGVVARERRLMLSHAQRNFGALGDVLAVF
                     LFVFIGSLLSWHDLLAGLVLGAAVLAARSAMHMLVNLGIARVSGTTFRKGVLTGLALM
                     PMTAFAMLLLEESRLYGFDLARESLPAIVGLLVLLDVVGPVVTQRALMVAGEAHPEEG
                     "
BASE COUNT          527 a         1282 c         1327 g          558 t
ORIGIN      
        1 ggaaggccac ggccatcagg ctgatcacca tctgatgccc gttgaccatc tccatcacga
       61 tgatcgcggc ggtgatcggc gactgcgtca ccgcggccag gaacgcggcc atgcatagcg
      121 cgatccaggc atgcgggtcg acgcccgcgc cgacccactg cgcgaggtcg ctgccgaccg
      181 ccgtgcccac cgccagcgac ggcgcgaaga taccgcccgg catgccactg aagtaggtcg
      241 cgatcgtggc cgcgaagcgg gtgatcggcg tgtgccactc gagcgtcagc tggccgccga
      301 tggcctgcga ggcggccgca tagccgctgc cgaacgaggt gccgccgctg acccaaccca
      361 gggccgcgac gaccaggccg cacaggcccg cgaacgccac cgggtgcgcg cggcgccagg
      421 tccacagccg gcccatcgga tggcgctgcg gccacagcag caaccagctg aacaggccgc
      481 cgagcacgcc gcagaccaag ccggcgatca gggtcggcag cagcaccgcg gtgccgaggt
      541 cgcgcaccgt gagctgaccg aagtacgcgt agttgccctt cagcgcgatc gccaccagcc
      601 ccgagatgat gatggtgctg accagcacgc cgctggtgcg gctctccagg cgccggccga
      661 gctcctcgac ggcgaacacg atgccggcca gcggcgtgtt gaacgccgcg gcgatgccgg
      721 ccgcgccgcc ggccaggatc aggtccgacg aacgcagcgc gcggccgtgc ggcagaaagc
      781 ggttcgccag gtgcagcacc gaggccgcga cctgcaccga cgggccttcg cgccccgccg
      841 agaaaccacc gacgagcgcg agcgatccca acagcacctt cgccgaagcg acgcgcagcg
      901 acagcagcgc ctggaccgat cggccgagcg cggccaggcg ggtcgccgcc accacctgcg
      961 ggataccgct gccggcggca ccgggcacgt aacgttgcgt ggcccagacg acaagcatgc
     1021 cgatcgacgg cgcgagcacg aacggcgccc acggccagcg cgtgtgaatg gtgtcgaaca
     1081 gcatcagcgc gtgctcggcc agcgtcgcga agccgaccac cgccaagccg gccgcgccgg
     1141 cggcggccca cagcaccgcg cgcacttgcc agcgctggcg cacgcggcga ttcgtcagga
     1201 acgcgagacg gcgctgcggg agcatcgacg acgctcggcc aggcccgcgc gcagcttcgc
     1261 tcaacgcttc acgtagcgga tgtcggcgcg gcggttctcg gcccatgccg cctcgtcgtg
     1321 gccttcggcc ttggggcgct cctcgccgta gctgacggcc tcgatctgcg ccggcttcgc
     1381 gcccatcagc tcgagctgct tcgcgacgat ctgcgcccgc ttgtcgccca gcgccaggtt
     1441 gtattcgcgc gagccgcgtt cgtcggcgtt gccctcgacg cggatctgca ccgcggcgtg
     1501 cgtctgcagg aactggccgt gcgcctgcac cagcggctgg tacttgggat cgaccgtgaa
     1561 gtcgtcgtag gcgaagtaga cgctgcgcgc cgcgagctgg ctcttcggat cgtcgagcgg
     1621 gtcgagcgtg accgtggcga cgctgctctg cgcggtgctg gctgccggcg gtgttgccgg
     1681 cggcgcggtg ctcgcgctcg cgaccggcgc cttggtgtcg atcggcgtgc tgctgcaggc
     1741 ggccagcgcg gccgcggcga tcagcgcgat ggaaaggctc ttgatggtca tggaaggact
     1801 cctggggttg cgatcgggtc gggacgaccc gctcattcgg taaaaggcag cggctttgcg
     1861 ccccgtgcga cgtcgagccc gcccatgcag tcgacgagca cggccaggcc gtcgtgcacc
     1921 ttgcgcagct gggccgcgct catgcgctcg agcacattcg gcagcaagcc gcgtgccggg
     1981 ccgggtgcgc gcttcaggcc gcgccggccg gcggccgtca gatggacttg cacgatgcgc
     2041 tggtcctgcg gcgagcggcc cttgcgcacc aggccggcgg cgtcgagccg ggtcaacagg
     2101 ttgctgacgg tggtctggtg catgcgcagg cgctgcgcca gttcgcccac gcttaggccg
     2161 tcggcctgcg ccacttcgtg cagcgcccag agctgcgcgc cggggatgcc ggtcgagcgc
     2221 tcgagccgtg ccgagtggct gccggccagg cgcagcacga cgcgcagctg ctgcagcaca
     2281 tcgagtccgc cgggcgcacc ccggcgccgg cgcggcgcgg tcacgtctga tttcatttgc
     2341 gcaaattata ttgcgtcaaa tgtaatcaaa ttgttgcaaa agaaaggcca ctcccatgaa
     2401 gttgagatga tgcggcgcgg taggctgcgc gccgcgcgac aacaaccaga aggcgtaaac
     2461 ggatgaacac ggagttgctc ggtccgctcg tggcctggcc gttgatgctg ctcgcggcct
     2521 ggtcggccgg cgagtgggtg ttccggcact ggaacctgcc gcgcgtggcg gcctacggcg
     2581 cgatcgggtt gctgctcggc ggtgtcgggc tgtcgcagaa cgtggcgtcg caggcggcgc
     2641 agggcttcat ggcgaatgcg gcgctggcgc tgaccctgtt cgagctcggc taccgcatca
     2701 acccgcgctg gttccgccac aacccctggg tgctcgcggc cggcctggtg caggcgctgc
     2761 tgacgttcgg tgcggtgttc tgggtcgccg gcgcgttcga cgtgccgctc gatcaccgcc
     2821 tcgtgctcgc cgcgctgtgc gtcgcgacct cgccggccgc ggtgatgcgc gtcacgcacg
     2881 agctgcgcag cgccggccag gtgaccgagc ggctgctgca cctgtgcgcg ctgaactgcc
     2941 tggtggccgt gctgttgctc aagctcgtcg tcggctactg gcacctcgag accagcggcg
     3001 atctcggcaa ggccgcgttc aacagcctgt acgtgatcgg cctgtcgctg gtggcgggcg
     3061 cgaccttcgg cgtcatcgca ccgttcctga atcgcatcgc cacgagcagc cagagcacga
     3121 ccgtgacctt cgcgttcctg gtgctgctgc tgacaggcat cgcgcagacg ctgcacctgt
     3181 cgccgctgct ggccgcgctc gcgttcggcg tcgtggcgcg cgagcgccgc ctgatgctca
     3241 gccacgcgca gcgcaacttc ggcgcgctcg gcgacgtgct cgcggtgttc ctgttcgtct
     3301 tcatcggcag cctgctgtcg tggcacgacc tgctcgccgg actggtgctc ggcgcggcgg
     3361 tgctggcggc gcgcagcgcg atgcacatgc tggtgaactt gggtatcgcg cgggtgagcg
     3421 gcacgacctt tcgcaagggc gtgctgaccg gtctggcgct gatgccgatg acggcgttcg
     3481 cgatgttgct gctcgaggag agccggctct acggcttcga cctggcacgc gagagcctgc
     3541 cggcgatcgt cggcctgctc gtgctgctcg acgtagtcgg cccggtcgtc acgcagcggg
     3601 cgttgatggt ggccggcgag gctcacccgg aggaaggcta gagatgacat gcgcgacgaa
     3661 cggtcaatcg aactgcagag cggcggcttg cggc
//
LOCUS       DATMJB010000372         9203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_884965, whole genome shotgun sequence.
ACCESSION   DATMJB010000372 DATMJB010000000
VERSION     DATMJB010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884965"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VNU48_08045"
     CDS             complement(<1..288)
                     /locus_tag="VNU48_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HWI11264.1"
                     /translation="MLFNTVLFTGNANPALAQEIATNLGIELGKAKVGRFSDGEVDIE
                     IQQNVRARDIFVVQPTCAPTNENLMELCIMVDALKRASARRVTAVIPYFGYA"
     gene            complement(342..418)
                     /locus_tag="VNU48_08050"
     tRNA            complement(342..418)
                     /locus_tag="VNU48_08050"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(382..384),aa:Gln,seq:ttg)
     gene            complement(464..1360)
                     /gene="ispE"
                     /locus_tag="VNU48_08055"
     CDS             complement(464..1360)
                     /gene="ispE"
                     /locus_tag="VNU48_08055"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWI11265.1"
                     /translation="MRALYDVAAPAKLNLFLHVVGRRADGYHLLQSLFVLIDWCDTLQ
                     FERRSDGHLVRHDLGPALPADDLCLRAARALQTASGTPLGADISIAKTVPWGAGMGGG
                     SSDAASTLLALNRLWGLNWPRARLATLALTLGADVPFFVGGRNAIVEGIGERLTPVPA
                     PAQWLAVVKPPVGIETRAIFGSPLLVRNTEPAILESFAANGQQNDQLEVMKEVMTPGF
                     GANDLQAAAEAECAQVVQAASMLHRRFGNSRMTGSGSAVFARAGTGDQPEAAMPADLA
                     PGWIGRMCRSLGEHPLVGWAAD"
     gene            complement(1446..1985)
                     /locus_tag="VNU48_08060"
     CDS             complement(1446..1985)
                     /locus_tag="VNU48_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HWI11266.1"
                     /translation="MSARTAAAALLAALVLASCASVPQGAPPVGEALSGRLAVRVDGG
                     DGGAPRSENAAFELQGSPRAGRLNLSTPLGSVIAQARWAPGSVVLATPQGERSFADLD
                     ALTREVLGESVPVAALFDWLRGRPWPGAASTASSAPAEPGFAQLGWAVSLARFDEGWV
                     VARRERAPAVTVRAKLDRP"
     gene            complement(1982..3799)
                     /locus_tag="VNU48_08065"
     CDS             complement(1982..3799)
                     /locus_tag="VNU48_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI11267.1"
                     /translation="MPTLNPRTGRALALPALLASLLLLATGAHAQDAQPAPAAPERVE
                     NSAMNAPLFQQLLIGEIELREGDLAAAYQLILDAARRTKNEQLFRRATDIALQARAGD
                     EALAAVKAWRLTLPDSVDALRYQVQLLVQLNRTAEVVEPLRLLIKATPATERPALISA
                     LPRLLVRSSDHNQAAQIIEQVLLPYADAPETRVAARVAIGRGWLAAINGEKALALARS
                     AHELDRSAEAPAGLALEMMPGTPAAEEMVKDHLAAMPGSNAVRLLYVRTLLASQRLAD
                     ATGQLETLTQNAPNLAQAWLILGALHVQLREPVPATAALQKYIALVQTTDMPAAPAPA
                     PAADADEDDAVPASKDDALTRGYLLLSQAADQQGDFKGAEAWLAKIDNPKRALEVQAR
                     RASLLAREGKVAQARELIRRLPEKTPEDARAKVLAEAQVLRDAKLWRDANDVLTQGNK
                     KFPDDVDLIYEQSMMAEKLNRLADMERLLRRVIELKPDHQQAYNALGYSLAERNLRLP
                     EARSLIKKALELSPGEPSITDSLGWVEYRLGNRDEAVRLLREAYRGQPDAEIAAHLGE
                     VLWVNGQTDEARRIFGEARKRDADNDVLRETLARLRVDL"
     gene            3874..4689
                     /gene="mutM"
                     /locus_tag="VNU48_08070"
     CDS             3874..4689
                     /gene="mutM"
                     /locus_tag="VNU48_08070"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HWI11268.1"
                     /translation="MPELPEVEVTRQSFADRIRGARVQAVRLGKPLRWPLGCAPESLV
                     AQVVGEVTRRGKYLWLALDRGGLLLHLGMSGSLAFDTRAAAPGPHDHFDLVTARGTLR
                     LTDPRRFGAVVWSSALDRGAAAKLLAGLGLEPFDPAFDGAHLHAALRGRRVAIKQALL
                     GGAIVVGAGNIYACEALFEAGIDPRTRGDRVSLARCERLAAAVRSTLTQALELGGSTL
                     RNFRDAHGMAGEFQNQARVYGRAGLACMRCDGTIRRIVQGQRATYFCPGCQRR"
     gene            4818..6827
                     /locus_tag="VNU48_08075"
     CDS             4818..6827
                     /locus_tag="VNU48_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HWI11269.1"
                     /translation="MTPSFAISLDALSGWRMTLGRRLDDVSRYLADRELVDPPAAAQL
                     TALRERLGNEKLVVAFVAEFSRGKSELINAIFFADTGRRVLPATPGRTTMCPVELAWD
                     GDENAALMLLPIETRLEGLSLGELRMQRRAWTIRPLQISDPERLAEALQEVTRTKRVA
                     KDQARELGFWDDDTPDDNPPVDDENRVEVPAWRHALINYPHPLLKQGLVVLDTPGLNA
                     IGAEPELTLSLLPSAHATVFILGADTGVTKSDLGVWRDHLGTHALSRFVVLNKIDALR
                     DPLATALQVDAQITAQCVETARTLGIGVERVFPLSARQALAARVDGDAAGLQESRLPA
                     LEAALVAQLLPQRRQVLEQAVLEGAQQLDTHVARRLGDQRRQLAEQMLELRGLRGKSA
                     GKLRLMLERVDLETAEFEQCTVRLHALRTVHSRMLRDALIRLSSDRLRDEVAAMQTQM
                     SASLLNLGAKKAFVALCARLRALLDDAQQRSNEIRDMLGASFGKLNGEFGFGLAMTKA
                     PDLARFADELREVENGYVQYLGLSQALRLAQPKFLEQFRRMLMSKLRVVFESASAELE
                     LWNKATSSQVDSQLRERRRNFRRRRESLERVQTAASELELRIVELETQDERAQAALRR
                     ARQLFDALREQATAGPVAGNDLISLQLPAAADREADGGARRRAQA"
     gene            6814..7887
                     /gene="mutY"
                     /locus_tag="VNU48_08080"
     CDS             6814..7887
                     /gene="mutY"
                     /locus_tag="VNU48_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HWI11270.1"
                     /translation="MRRPEPTPAFAASLIAWQRAHGRHTLPWQNTRDPYRVWLSEIML
                     QQTQVTTVLGYYERFLQRFPDVAALAAASLDEVFALWSGLGYYSRARNLHRCAQAVVE
                     QHGGRFPRSSAALAQLPGIGRSTAAAIAAFCFGERVAILDGNVKRVLTRVLAFDADLA
                     EVKHERVLWERATALLPERDIGRYTQGLMDLGATLCTQRAPGCLLCPVHAHCAAARAG
                     TPERYPVKTRRLKRGTREHVWLRLQWRDEVWLVQRPSTGVWAGLWSLPEFDSHAAFET
                     ASGAWPGRGEALASFTHTLTHLDWTLHPVLWQLPDRLGAARRDALTAAWPSGRWFGLD
                     EALAAGVPAPLRRLLTPAGAALR"
     gene            complement(7892..8842)
                     /gene="rapZ"
                     /locus_tag="VNU48_08085"
     CDS             complement(7892..8842)
                     /gene="rapZ"
                     /locus_tag="VNU48_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWI11271.1"
                     /translation="MSTSPDPAPAAPPPGASPREVVLVSGISGSGKSVALHALEDAGF
                     FCVDNLPPELLRDFLRLEQGRQDRRLAIAVDVRSAASLPHLLPLIQQLRSEGVAIQVV
                     FLDASTDALVRRFSETRRPHPLSADPPRGSPPRRAEDHVPDERRALVDAIELERELLA
                     ELRELATVIDTSQLRSAQLRLWMRDLVRAGHNRLTLVFESFAFKHGVPLDADYVFDVR
                     VLPNPHWVPELQTLTGRDEPVAAYLRAQPEVGEMLAQVEAFLARWLPAFEADQRSYLT
                     VAIGCTGGQHRSVYFAEVLAQHFSARSATLIRHRELDARG"
     gene            complement(8839..>9203)
                     /locus_tag="VNU48_08090"
     CDS             complement(8839..>9203)
                     /locus_tag="VNU48_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HWI11272.1"
                     /translation="GDAAAGTLIFDEIDAGVGGAVAETVGRLMKQLGRDHQVLAVTHL
                     PQVAACADHHFVVSKQSRDGLTLSEVGAVSGEVRVAEVARMLGGERLSNTSLAHAQEM
                     LAVGADAAAPAARARKRS"
BASE COUNT         1282 a         3337 c         3265 g         1319 t
ORIGIN      
        1 cgcgtagccg aaatacggaa tcaccgcggt gacgcggcgc gccgaggcgc gcttgagcgc
       61 gtcgaccatg atgcacagct ccatcaggtt ctcgttggtc ggcgcgcagg tcggctgcac
      121 gacgaagatg tcgcgcgcgc ggacgttctg ctggatctcg atgtcgactt cgccgtccga
      181 gaaacggccg accttcgcct tgccgagctc gatgccgaga ttggtcgcga tctcctgcgc
      241 cagcgcaggg ttcgcgttcc cggtaaacag cacggtattg aacagcacga gaggctccgt
      301 cgaggcatgc cggcctgggc gcgcgccggg atcaggtgat ttggcagggg aggaaggact
      361 cgaaccctcg catgtcggaa tcaaaatccg atgccttaac caacttggcg actcccctac
      421 acaggtcgac agacttctca tctgcccacc gcaaccacgc tcatcagtct gcggcccacc
      481 ccaccagcgg gtgttcaccc aggctgcggc acatccggcc gatccagccc ggcgcaagat
      541 ccgccggcat cgccgcctcg ggttggtcgc cggtgcctgc tctcgcaaac accgcgctgc
      601 ccgaaccggt catgcggctg ttgccgaaac gccggtgcag catcgaagcc gcctgcacca
      661 cctgcgcgca ttctgcctcc gccgcggcct gcagatcgtt ggcgccgaaa ccgggcgtca
      721 tgacctcttt catgacctct agctggtcgt tttgctggcc atttgcagcg aagctttcaa
      781 gtatagcagg ttcggtgttt cggaccagca atgggctgcc gaaaatggca cgggtttcga
      841 tgccgacggg aggcttgacg accgccagcc actgcgccgg ggccggcacc ggggtgagcc
      901 gctcgccgat cccttcgacg atcgcgttgc gcccgccgac gaagaacggc acgtcggcgc
      961 ccagcgtcag cgcgagcgtg gccagccgcg cgcgcggcca gttcaggccc cacaggcggt
     1021 tcagcgcgag cagggtcgag gccgcatcgg aacttccgcc gcccatgccg gcgccccagg
     1081 gcacggtctt ggcgatcgag atgtcggcgc ccagcggtgt gccgctcgcg gtctgcagcg
     1141 cccgcgcggc gcgcaggcac aggtcgtccg ccggcagcgc cgggccgagg tcgtggcgga
     1201 caaggtggcc gtcgctgcgg cgctcgaact gcaaggtgtc gcaccagtcg atcagcacga
     1261 acagcgactg cagcaggtgg tagccgtcgg cacgccggcc gacgacatgc aggaacaggt
     1321 tgagcttggc cggcgcggcg acgtcgtaga gcgcgcggat gctcatgacg accccttgtc
     1381 cgatgggtac atcggccggt cccccgaggg gacgagcctt gcttggggtg gcccggcgcg
     1441 gcggctcatg gccgatcgag cttcgcacgc acggtgaccg ccggcgcgcg ttcgcgccgc
     1501 gccacgaccc agccctcgtc gaaacgcgca aggctcacgg cccagccgag ctgcgcgaag
     1561 ccgggttccg ccggcgcgct gctggcggtg ctcgcggcgc cgggccaggg ccggccgcgc
     1621 agccaatcga acagcgcggc gaccggcacg ctctcgccga gcacttcgcg cgtcagcgcg
     1681 tccaggtcgg cgaagctgcg ctcaccctgc ggcgtggcca gcacgaccga gccgggcgcc
     1741 cagcgtgcct gcgcgatgac gctgcccaac ggcgtcgaca gattcaggcg gcccgcgcgc
     1801 gggctgccct gcaactcgaa ggccgcgttc tcggaacgtg gcgcgccacc atcgccgccg
     1861 tcgacgcgca ccgccagacg cccggagagc gcctcgccca ccggcggcgc accttgcggc
     1921 accgacgcgc acgacgccag cacgagcgct gccagcagcg ccgcggccgc ggtccgtgcg
     1981 ctcacaggtc gacgcgcagc cgcgccagcg tctcgcgcag cacgtcgttg tcggcgtcgc
     2041 gcttgcgcgc ctcgccgaag atgcgccggg cttcgtcggt ctggccgttg acccacagca
     2101 cttcgcccag atgcgcggcg atctcggcgt cgggctggcc gcggtaggcc tcgcgcagca
     2161 gccgcacggc ttcgtcgcgg ttgcccagtc ggtattcgac ccagcccagg ctgtcggtga
     2221 tcgaaggttc gccggggctg agctccagcg ccttcttgat caggctgcgc gcttccggca
     2281 ggcgcaggtt gcgttctgcc agcgagtagc cgagcgcgtt gtaggcctgt tggtgatcag
     2341 gcttgagctc gatcacgcgg cgcaacagcc gctccatgtc ggccaggcga ttcagcttct
     2401 cggccatcat cgactgctcg tagatcaggt cgacgtcgtc ggggaacttc ttgttcccct
     2461 gcgtgagcac gtcgttcgca tcgcgccaca gcttcgcgtc gcgcagcacc tgggcctcgg
     2521 cgagcacctt ggcgcgcgcg tcctcggggg tcttctcggg caggcggcgg atcagctcgc
     2581 gcgcctgcgc gaccttgccc tcgcgggcca gcagcgaggc acgccgcgcc tgcacctcga
     2641 gcgcgcgctt cgggttgtcg atcttcgcga gccaggcctc cgcgcccttg aagtcgccct
     2701 gctggtcggc ggcctgcgac agcagcaggt agccgcgcgt cagcgcgtcg tccttcgatg
     2761 ccggcacggc gtcgtcttcg tcggcgtcgg ccgcaggtgc gggagcgggg gcagcgggca
     2821 tgtccgtcgt ctgcaccagc gcgatgtact tctgcagcgc cgcggtcgcc ggcaccggct
     2881 cgcgcagctg gacatgcagc gcgcccagga tcagccaggc ctgcgccagg ttcggcgcgt
     2941 tctgcgtgag cgtctcgagc tgcccggtcg catcggccag gcgctgcgat gccagcaggg
     3001 tgcgcacgta gagcaggcgc accgcgttgc tgcccggcat cgcggccaga tgatccttca
     3061 ccatctcttc ggcggccggc gtgccgggca tcatctcgag cgcgagaccg gccggcgcct
     3121 cggcactgcg gtccagctcg tgcgcgctgc gcgccagcgc gagcgccttc tcgccgttga
     3181 tcgccgcgag ccagccgcgg ccgatcgcga cgcgcgccgc gacgcgggtc tcgggcgcgt
     3241 cggcgtaggg cagcagcacc tgctcgatga tctgcgcggc ctggttgtgg tcgctgctgc
     3301 gcacgagaag acgcggcagc gcgctgatca gcgccgggcg ttcggtcgcc ggcgtcgcct
     3361 tgatcagcaa gcgcagcggc tcgacgacct cggcggtgcg gttcagctgc accagcagct
     3421 gcacctggta gcgcagcgcg tcgaccgaat cgggcagcgt cagccgccac gccttcaccg
     3481 ctgccagcgc ctcgtcgccg gcgcgggcct gcaatgcaat gtcggtggcg cggcggaaca
     3541 gctgctcatt cttcgtgcgg cgcgccgcat cgaggatcag ctggtaggcc gccgcgaggt
     3601 cgccttcgcg cagttcgatc tcgccgatca gcaactgctg gaacagcggc gcgttcatcg
     3661 ccgagttctc gaccctctcg ggcgcggccg gcgccggctg cgcgtcctgc gcgtgggccc
     3721 ctgtggcgag cagcagcagc gaagccaaca acgcaggcag cgccaacgca cggccggttc
     3781 gggggttgag ggtcggcatc gagtctctcc agaagctgcg tttcattcta ggtcgccggg
     3841 gtgcgaccgc tcgggcgccc gataattccc cgcatgcccg aactccccga agtcgaggtc
     3901 acgcgccaga gcttcgccga ccgcatccgc ggtgcgcgcg tgcaggcggt gcgcctgggc
     3961 aaacccctgc gctggccgct gggctgcgcg cccgaatcgc tggtcgcgca ggtcgtcggc
     4021 gaggtcacgc ggcgcggtaa gtatttgtgg ctcgcgctgg accgtggcgg cctgctgttg
     4081 cacctgggca tgtcgggttc gctcgcgttc gacacgcgcg ccgcggcccc gggcccgcac
     4141 gaccacttcg acctcgtcac cgcgcgcggc acgctgcgcc tgaccgatcc gcggcgcttc
     4201 ggcgcggtgg tctggtcgag cgcgctcgac cgcggcgcgg ccgcgaagct gctcgccggc
     4261 ctcggcctcg aacccttcga cccggccttc gacggcgcgc acctgcacgc cgcgttgcgc
     4321 ggccggcgcg tcgcgatcaa gcaggcgctg ctgggcggtg cgatcgtcgt cggcgccggc
     4381 aacatctacg cctgcgaggc gctgttcgag gccggcatcg acccgcgcac gcgcggcgac
     4441 cgcgtcagcc tggcgcgctg cgagcggctc gccgcggcgg tgcgcagcac cttgacgcag
     4501 gccctcgaac tcggcggctc gacgctgcgc aactttcgcg acgcgcacgg catggccggc
     4561 gagttccaga accaggcgcg tgtctatggt cgcgcggggc tggcctgcat gcgttgcgac
     4621 ggcaccatcc ggcgcatcgt tcagggccag cgcgcgacct atttctgccc gggctgccaa
     4681 cgccgctaac cacaattgcg cattgcgtcg cgcttcgtcg cgaactcgtc ggtgagcaga
     4741 tgtttcgcgt gcgcgcacac cggacccttg cgctacgatg gcccgccatc gcgaccccac
     4801 gcccacaacc cgcagccatg acgccttcgt tcgccatcag cctcgatgcc ctcagcggct
     4861 ggcggatgac gctcgggcgg cgcctcgacg atgtctcgcg ctacctcgcc gatcgcgagc
     4921 tggtcgaccc ccccgcggcc gcgcagctga ccgcgttgcg cgaacgcctg ggcaacgaga
     4981 agctggtcgt cgccttcgtc gccgagttct cgcgcggcaa gtcggagctg atcaacgcga
     5041 tcttcttcgc cgacaccggc cgccgcgtgc tgccggccac acccgggcgc acgacgatgt
     5101 gcccggtcga actcgcctgg gacggcgacg agaacgcggc gctgatgctg ctgccgatcg
     5161 agacgcggct cgaaggtctg tcgctcggcg agctgcgcat gcagcgccgc gcgtggacga
     5221 tccggccgct gcagatcagc gaccccgagc gcctggccga agcgctgcag gaggtcaccc
     5281 gcacgaagcg ggtcgcgaag gatcaggcgc gcgaactcgg cttctgggac gacgacaccc
     5341 ccgacgacaa cccgccggtc gacgatgaaa accgggtcga ggtgccggcc tggcgccacg
     5401 cgctgatcaa ctacccgcac ccgctgctga agcagggcct ggtcgtgctc gacacgccgg
     5461 ggctgaacgc gatcggcgcc gagcccgagc tgacgctgag cctgctgccg agcgcgcacg
     5521 ccaccgtctt catcctcggc gccgacaccg gcgtgaccaa gtccgacctc ggcgtctggc
     5581 gcgaccacct cggcacgcac gcgctgtcgc gcttcgtcgt gctgaacaag atcgacgcgc
     5641 tgcgcgaccc gcttgccact gccctccagg tcgacgcgca gatcaccgcg caatgcgtcg
     5701 agacggcgcg cacgctcggg atcggcgtcg aacgcgtgtt cccgctgtcg gcgcggcagg
     5761 cgctcgcggc gcgcgtggac ggcgacgccg cgggcctgca ggagagccgc ctgcccgcgc
     5821 tcgaagcggc gctggtcgcg cagctgctgc cgcaacgccg ccaggtgctc gagcaggccg
     5881 tgctcgaggg cgcacagcag ctcgacaccc atgtcgcgcg gcgtctgggc gaccagcgcc
     5941 gccagctcgc cgagcagatg ctcgaactgc gcggcctgcg tggcaagagc gccggcaagc
     6001 tgcgcctgat gctggaacgg gtcgacctcg agaccgccga attcgagcag tgcacggtgc
     6061 gcctgcacgc gctgcgcacg gtgcacagcc gcatgctgcg cgacgcgctg atccggctgt
     6121 cgtccgaccg gctgcgcgac gaggtcgcgg cgatgcagac gcagatgagc gcgtcgctgc
     6181 tgaacctggg cgcgaagaag gcgttcgtcg cgctgtgcgc gcggctgcgc gcgctgctcg
     6241 acgatgcgca gcagcgcagc aacgagatcc gcgacatgct gggcgcgtcg ttcggcaagc
     6301 tcaacggcga gttcggcttc gggctggcga tgacgaaggc gcccgacctg gcgcgcttcg
     6361 ccgacgagct gcgcgaggtc gagaacggct acgtgcagta cctcggcctg agccaggcct
     6421 tgcgcctggc acagccgaag ttcctcgagc agttccgccg catgctgatg tccaagctgc
     6481 gcgtcgtgtt cgagagcgcg agcgccgagc tcgagctgtg gaacaaggcg acctcgtcgc
     6541 aggtggattc gcagctgcgc gagcggcggc gcaatttccg gcgccggcgc gaatcgctcg
     6601 agcgcgtgca gacggcggcc agcgagctcg aactgcgcat cgtcgagctc gagacgcagg
     6661 acgagcgcgc gcaggccgca ctgcggcgcg cgcgccagct cttcgatgcg ctgcgcgagc
     6721 aggcgaccgc cggcccggtc gcgggcaacg acctgatctc gctgcagctg ccggccgcgg
     6781 ccgatcgcga agccgacggc ggcgcgcggc gacgtgcgca ggcctgagcc gacccccgcg
     6841 ttcgccgcgt cgctgatcgc ctggcagcgc gcgcacggcc ggcacacgct gccgtggcag
     6901 aacacgcgcg acccgtaccg cgtctggctg tccgagatca tgctgcagca gacgcaggtc
     6961 acgaccgtgc tcggctacta cgagcgtttc ctgcagcgct tcccggacgt ggccgcgctc
     7021 gccgcggcct cgctcgacga ggtgttcgcg ctctggagcg gcctgggcta ctacagccgc
     7081 gcgcgcaacc tgcatcgctg cgcgcaggcg gtcgtcgagc agcacggcgg gcgctttccg
     7141 cgcagcagcg cggcgctcgc gcaactgccc ggcatcggcc gctcgacggc cgcggcgatc
     7201 gccgcgttct gcttcggcga gcgcgtcgcg atcctggacg gcaatgtcaa gcgcgtgctg
     7261 acgcgcgtgc tcgcgttcga cgccgacctc gccgaggtga agcacgaacg cgtgttgtgg
     7321 gaacgggcca ctgcgctgct gccagaacgc gacatcgggc gctacacgca gggcctgatg
     7381 gacctgggcg cgacgctgtg cacgcagcgc gcgccgggtt gcctgctgtg cccggtgcat
     7441 gcccactgtg cggccgcgcg cgccggcacg ccagagcgct acccggtcaa gacgcgcagg
     7501 ctcaagcgcg gcacgcgcga gcacgtctgg ctgcgcctgc aatggcgcga cgaggtctgg
     7561 ctggtgcagc gcccgagcac cggcgtgtgg gccggcctgt ggagcctgcc ggagttcgat
     7621 tcgcacgccg cgttcgagac cgcgagcggc gcctggcccg gccgcggcga ggcgctggcc
     7681 agcttcacgc acaccttgac gcacctggac tggacgctgc acccggtgct gtggcagctg
     7741 cccgatcgcc tgggcgccgc gcggcgcgac gcgctgacgg ccgcctggcc gagcggccgc
     7801 tggttcggcc tggacgaggc gctggccgcg ggcgtacccg ctccgttgcg aagactgctg
     7861 acgccggccg gcgccgcgct gcgctgacgc ctcagccgcg cgcgtcgagt tcgcggtggc
     7921 ggatcagcgt cgccgagcgc gcggaaaagt gctgcgccag cacctcggcg aaatacaccg
     7981 agcggtgctg gccgccggtg cagccgatcg ccaccgtcag gtagctgcgc tggtcggcct
     8041 cgaacgccgg caaccagcgc gcgaggaagg cctcgacctg cgccagcatc tcgccgacct
     8101 cgggctgcgc gcgcaggtag gctgcgaccg gctcgtcgcg gccggtcagg gtctgcagct
     8161 cgggcaccca gtgcgggttc ggcagcacgc gcacgtcgaa cacgtagtcg gcatcgagcg
     8221 gcacgccgtg cttgaacgcg aagctctcga acaccagcgt caggcggttg tggccggcgc
     8281 gcaccaggtc gcgcatccac aggcgcagct gggcgctgcg cagctggctg gtgtcgatca
     8341 cggtggcgag ctcgcgcagc tcggcgagca gctcgcgttc gagttcgatc gcgtcgacca
     8401 gcgcacggcg ctcgtcgggc acatggtcct cggcgcggcg cggcggcgaa ccgcgcggcg
     8461 gatcggccga caacgggtgc gggcggcgcg tctcggagaa acggcgcacg agggcatcgg
     8521 tgctggcgtc gagaaagacg acctggatcg cgacgccttc gctgcgcagc tgctggatca
     8581 gcggcagcag gtgcggcagc gaggcggcgc tgcgcacgtc gaccgcgatc gccaggcgcc
     8641 ggtcctggcg gccctgttcg agccgcagaa agtcgcgcag cagctccggc ggcaggttgt
     8701 cgacacagaa gaagccggca tcctcgagcg catgcagcgc gaccgacttg cccgagccgg
     8761 agatgccgct gaccagcacc acctcgcgcg gcgacgcacc cggtggcggc gcggcgggcg
     8821 ccgggtctgg cgacgtgctc atgatcgctt gcgtgcgcgc gccgccggtg ccgccgcgtc
     8881 ggcaccgacc gcgagcatct cctgcgcatg cgccaggctc gtgttcgaca gccgctcccc
     8941 gcccagcatg cgcgcgacct cggcgactcg cacctcgccg ctgaccgcgc cgacctcgct
     9001 cagggtcagc ccgtcgcgcg actgtttcga gacgacgaag tgatggtcgg cgcaggccgc
     9061 gacctgcggc aggtgcgtga ccgcgagcac ctggtgatcg cggccgagct gcttcatcag
     9121 ccggccgacc gtctcggcga ccgcaccgcc gacaccggca tcgatctcat cgaagatcag
     9181 cgtgccggct gcggcatcgc cgg
//
LOCUS       DATMJB010000373         3011 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_651554, whole genome shotgun sequence.
ACCESSION   DATMJB010000373 DATMJB010000000
VERSION     DATMJB010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651554"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VNU48_08095"
     CDS             complement(<1..1028)
                     /locus_tag="VNU48_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HWI11273.1"
                     /translation="MSSSPFATPAITSRLPQVGTTIFTVMSALALEHGAVNLGQGFPD
                     FDCDPRLVDAVDAAMRAGANQYPPMAGVPALREAVAAKLGAMYGHAYDPGSEITITAG
                     ATQAIITAVLAIVHPGDEVIVLDPCYDSYEPNIELAGGTAVHVPLTPGSFEPDFARIA
                     AAITPRTRAIIVNTPHNPSATVWSAEQMARLADTLRGTDIIVIADEVYEHMVFDGTAH
                     QSVARFPELAQRSFVVSSFGKTYHVTGWKVGYVAAPAPLMAEFRKVHQFNVFTVNTPV
                     QHAIARYMADPAPYLGLAAFYQRKRDLFRAGLAATRLRVLPSRGSYFQCVDYAAVSDL
                     PEADFCRFL"
     gene            complement(1043..1987)
                     /locus_tag="VNU48_08100"
     CDS             complement(1043..1987)
                     /locus_tag="VNU48_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HWI11274.1"
                     /translation="MNLHQFRFVQEAVRRDLNLTETAKALFTSQPGVSKAILELEAEL
                     GVDIFARHGKRLRRITEPGREVLKSIELIMREVGNLKRIGEEFSMQDAGTLSIATTHS
                     QARYFLPEPVAQLRKRFPKVNISLHQGSPEQVARMVIDEVADLGIATEGLTGYDELVT
                     LPCYEWRHAVVMPAGHALAQVERISLEDLAVLPLVSYHPSFSGRTRVDQAFATRNLKP
                     NIVLEAIDADVIKTYVRIGLGVGIVAELAVRDDPPGGDLVSRPVGHLFGNNVTRLAFK
                     RGAYLRNFVYAFAEMLSDRLSRKLIAGAMAGDADDYQL"
     gene            complement(1984..2364)
                     /locus_tag="VNU48_08105"
     CDS             complement(1984..2364)
                     /locus_tag="VNU48_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HWI11275.1"
                     /translation="MKRGLLLFAHGARDPRWAEPFEAVAQRVRLRDPTLAVQLAFLEF
                     MAPSLREAGEALAAAGCVRVDVLPLFLGAGGHVRKDLPELLADLRAAHPGTAWHLRRA
                     IGEVDSVIDAMAHAALQSTTETRP"
     gene            complement(2480..>3011)
                     /locus_tag="VNU48_08110"
     CDS             complement(2480..>3011)
                     /locus_tag="VNU48_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HWI11276.1"
                     /translation="SAAQALVDSRSTWQLSEVSVTRWLLPPGEAPTVREEKLPSLTWT
                     SSLSSAVVAAAVLPLDTMSTFELRRYVQHLALNEQAAQRYEIQFWKRALYPFACLVMM
                     ALALPFAYLHARAGGISLKVFGGIMLGISFVLLNNVAGHLGLLRNWTPWMVAAAPSAL
                     YLLLSFAAFSWLVRYR"
BASE COUNT          456 a         1005 c         1104 g          446 t
ORIGIN      
        1 aggaaacggc agaagtcggc ctcgggcaag tcgctgacgg cggcgtagtc gacgcactga
       61 aagtagctgc cgcggctcgg gagcacgcgc agcctggtcg cggcgaggcc ggcgcggaac
      121 aggtcgcgct tgcgctggta gaacgcggcg agcccgaggt agggcgcggg gtcggccatg
      181 tagcgcgcga tcgcgtgctg caccggcgtg ttgacggtga acacgttgaa ctggtgcacc
      241 ttgcggaact cggccatcag cggtgccggc gccgccacgt agccgacctt ccagccggtc
      301 acgtgatagg tcttgccgaa gctcgagacc acgaagctgc gctgcgccag ttcgggaaaa
      361 cgcgcgacgc tctggtgcgc ggtaccgtcg aacaccatgt gctcgtagac ctcgtcggcg
      421 atgacgatga tgtcggtgcc gcgcagcgtg tcggcgagcc gcgccatctg ctcggccgac
      481 cagacggtgg cgctcgggtt gtgcggcgtg ttgacgatga tcgcgcgcgt gcgcggcgtg
      541 atcgccgcgg cgatgcgcgc gaagtccggc tcgaagctgc ccggcgtcag cggcacgtgc
      601 accgcggtgc cgccggcgag ttcgatgttc ggctcgtagc tgtcgtagca ggggtcgagc
      661 acgatcacct catcgcccgg gtgcacgatg gcgagcaccg cggtgatgat cgcctgcgtc
      721 gcgccggcgg tgatcgtgat ctcgctgccg gggtcgtagg cgtggccgta catcgcgccg
      781 agcttcgcgg ccaccgcctc gcgcagtgcc ggcacgccgg ccatcggcgg gtactggttc
      841 gcgccggcgc gcatcgccgc gtcgaccgcg tcgaccaggc gcgggtcgca gtcgaagtcc
      901 gggaagccct ggccgaggtt gacggcgccg tgctcgagcg cgagcgccga catcacggtg
      961 aagatggtgg tgccgacctg cggcaggcgg ctcgtgatcg ccggggtcgc gaaggggctg
     1021 ctgctcatgc ggggtcgggt ggtcagagtt ggtagtcgtc ggcgtcgccc gccatcgcgc
     1081 cggcgatcag cttgcggctc agacggtccg acagcatctc ggcgaaggcg tagacgaagt
     1141 tgcgcaggta ggcgccgcgc ttgaacgcga gccgggtgac gttgttgccg aacagatgcc
     1201 cgaccgggcg cgataccaga tcgccgcccg gcgggtcgtc gcgcaccgcg agttcggcga
     1261 cgatgcccac gcccaggccg atgcgcacat aggtcttgat gacgtcggcg tcgatcgcct
     1321 cgagcacgat gttgggcttc aggttgcgcg tcgcgaaggc ctggtcgacg cgcgtgcggc
     1381 cgctgaacga cgggtggtac gacaccagcg gcagcacggc caagtcttcg agcgaaatgc
     1441 gctcgacctg cgccagcgcg tgcccggccg gcatcaccac cgcgtggcgc cactcgtagc
     1501 agggcagcgt gacgagctcg tcgtagccgg tgaggccttc ggtcgcgatg ccgaggtcgg
     1561 cgacctcgtc gatcaccatg cgcgccacct gctccggcga gccctggtgc aggctgatgt
     1621 tgaccttcgg gaagcgcttg cgcagctgcg ccaccggctc gggcaggaag tagcgcgcct
     1681 ggctgtgcgt ggtcgcgatc gacagggtgc cggcgtcctg catcgagaac tcctcgccga
     1741 tgcgcttcag gttgccgacc tcgcgcatga tcagctcgat gctcttgagg acttcgcggc
     1801 ccggctcggt gatgcggcgc aggcgcttgc cgtgacgcgc gaagatgtcg acgccgagct
     1861 cggcctcgag ctcgagaatc gccttcgaga cgcccggctg cgacgtgaac aaggccttcg
     1921 cggtctcggt gagattgagg tcgcggcgca ccgcctcctg gacgaaccgg aactggtgca
     1981 gattcatggt cgggtttcgg tcgtggactg caatgcggcg tgggccatcg catcgatcac
     2041 gctgtcgacc tcgccgatcg cgcgccgcag gtgccacgcg gtgccggggt gcgcggcgcg
     2101 caggtcggca agcagctcgg gcaggtcctt gcgcacatgg ccgccagcgc cgaggaacag
     2161 cggcagcacg tcgacgcgca cgcagccggc ggcggcgagc gcctcgccgg cttcgcgcaa
     2221 gctcggcgcc atgaactcga ggaacgcgag ttgcaccgcg agcgtcgggt cgcgcaagcg
     2281 cacgcgctgc gcgaccgcct cgaacggctc ggcccagcgc gggtcgcgcg cgccgtgcgc
     2341 gaacagcagc agcccgcgct tcatgtgagc ccctcgcccg acgagtacgt cggtcgatcc
     2401 cccgagggga tgcgggccgg cgaggtccac gaagtggtcc tcttcgtggc ccacggagcg
     2461 gccctgcgct cgacccgctt caccggtacc tcaccagcca gctgaacgcc gcgaacgaga
     2521 gcagcaggta cagcgcgctc ggcgcggccg cgaccatcca cggcgtccag ttgcgcagca
     2581 ggccgaggtg gccggccacg ttgttcagca gcacgaagct gatgcccagc atgatgccgc
     2641 cgaacacctt caggctgatg ccgccggcgc gtgcgtgcag gtaggcgaac ggcagtgcca
     2701 gcgccatcat caccaggcag gcgaacgggt acagcgcacg cttccagaac tggatctcgt
     2761 agcgctgcgc ggcctgttcg ttgagcgcca ggtgctgcac gtagcgacga agttcgaacg
     2821 tcgacatcgt gtccagcggc agcaccgccg cagcgacgac ggccgacgac aggctgctgg
     2881 tccaggtcag cgacggcagc ttctcctcgc gcaccgtcgg cgcttcgccg ggcgggagga
     2941 gccagcgggt gacgctcacc tccgacagct gccaggtcga acgcgagtcg accagcgcct
     3001 gcgcggccga g
//
LOCUS       DATMJB010000374         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_17909, whole genome shotgun sequence.
ACCESSION   DATMJB010000374 DATMJB010000000
VERSION     DATMJB010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17909"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..655
                     /locus_tag="VNU48_08115"
     CDS             287..655
                     /locus_tag="VNU48_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HWI11277.1"
                     /translation="MSYIDGFVIAVPTANKQKFIEHARHFDPLFIELGAIRVIEGWGD
                     GVPDGKVTDFRRAVQATAEETVAFAWVEWPDKATRDAAMKKMVEDPRMDPSTPGNPPM
                     PFDGKRMIFGGFEQVVEVKA"
     gene            complement(732..>2339)
                     /locus_tag="VNU48_08120"
     CDS             complement(732..>2339)
                     /locus_tag="VNU48_08120"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HWI11278.1"
                     /translation="QPLMRAVQVFGFHLATVDLRQSSDKHEAVVAELLRVAKVEADYG
                     ALAEDARRTLLLQQLNDARPLRVRAASYSEHTSGELAIFEAAVVMLQRYGRQALRHYI
                     ISHTEDVSDLLEVMLLLKEVGLLRGTLDDGAVSDLIVSPLFETIGDLRNAAGIMRAFY
                     ALPGVAAMTKRSGAEQDIMLGYSDSNKDGGSFTSNWELYRAEIELVQLFDALRAEHGL
                     TLRLFHGRGGTVGRGGGPSYQAILAQPPGTVNGQIRLTEQGEVIASKYAHPEIGRRNL
                     ETLVAATLEATLLHPTKSAPPAFLDAAAQISDASFRAYRKLVYETPGFTEYFFGATPI
                     REIAELNIGSRPASRKATRAIEDLRAIPWSFSWGQCRVALPGWCGFGSAIESFLGKDA
                     AKRKANLALLQKMHKQWPFFRTLLSNLDMVMAKSDLAIAARYVELVEDKRLGKKIFAL
                     VKAEWQRTNDALSLITGEKNRLDSNPSLARSIEHRFPYLDPLNHLQVELMRRYRQRTD
                     GDPENARVRTGIHISINGIAAGLRNTG"
BASE COUNT          369 a          777 c          804 g          389 t
ORIGIN      
        1 ttccaacctc ttcgacggcg ttccggaact acgacagcgg cggatcgccg tggcggatgc
       61 actgcaattt ccgcgcgccc gttgatgcca gtcaagccgc cacatcagtt tccggccagc
      121 agcggatgtt atcgaaaggc agtgctcggg cgagccagcg ccgccacagg acgcgcgccc
      181 gcgcaccggg cagacaatgt cgaatcggct tccttccgtt cgtcgtcgag gtacgaggcg
      241 ccctgaggag ggtgcgtcga ccggtcaacc caacaggagt caaggcatgt cctacatcga
      301 cggtttcgtg atcgcggttc caaccgccaa caagcagaag ttcatcgagc acgcgcgtca
      361 tttcgaccca ctattcatcg agttgggcgc gatccgtgtg atcgagggat ggggcgacgg
      421 tgtccccgac ggcaaagtca ccgatttccg tcgcgctgtc caggccacgg ccgaggaaac
      481 ggtggccttt gcgtgggttg aatggcccga caaggccacg cgcgacgccg ccatgaagaa
      541 gatggtggaa gacccgcgca tggacccgtc cacacccggc aatcccccca tgcccttcga
      601 tggaaagcgc atgatctttg ggggcttcga acaggtggtc gaagtgaagg cctgaacggc
      661 ggtgcgactc cgcacccgga cacgaacgtt gcgcaagctc agccggctgc gcgaagcttc
      721 cgcagccgcg ctcagccggt gttgcgcagc cccgccgcga tgccgttgat cgagatgtgg
      781 atgccggtgc gcacgcgcgc gttctcgggg tcgccgtcgg tgcgctggcg gtagcggcgc
      841 atcagctcga cctgcaggtg gttcaacggg tcgaggtagg ggaagcggtg ctcgatcgag
      901 cgcgccagcg acgggttcga atcgagccgg ttcttctcgc cggtgatcag cgacagcgca
      961 tcgttcgtgc gctgccactc ggccttgacc aacgcgaaga tcttcttgcc cagccgcttg
     1021 tcctcgacga gctcgacata gcgcgcggcg atcgcgaggt cgctcttggc catcaccatg
     1081 tcgagattcg acagcagcgt gcggaagaac ggccactgct tgtgcatctt ctgcagcagc
     1141 gcgaggttcg ccttgcgttt cgcggcgtcc ttgccgagga aggattcgat ggccgagccg
     1201 aagccgcacc agcccggcag cgcgacccgg cactggcccc agctgaacga ccaggggatc
     1261 gcgcgcaggt cctcgatcgc acgcgtcgcc ttgcgcgagg ccgggcgcga gccgatgttg
     1321 agttcggcga tctcgcggat cggcgtcgcg ccgaagaagt actcggtgaa gcccggcgtc
     1381 tcgtagacca gcttgcggta ggcgcgaaag ctggcgtcgc tgatctgcgc ggcggcatcg
     1441 agaaaggccg gcggcgcgct cttggtcggg tgcagcagcg tcgcctcgag cgtggccgcg
     1501 accagcgtct cgaggttgcg ccggccgatc tcggggtgcg cgtatttcga ggcgatcacc
     1561 tcgccctgct cggtcaggcg gatctggccg ttcaccgtgc ccggcggctg cgccaggatg
     1621 gcctggtagc tcgggccgcc gccgcgcccg accgtgccgc cgcggccgtg gaacagccgc
     1681 agggtcaggc cgtgctcggc gcgcagcgcg tcgaacagct gcaccagctc gatctcggcg
     1741 cggtacagct cccagttgct ggtgaagctg ccgccgtcct tgttgctgtc gctgtagccg
     1801 agcatgatgt cctgctcggc gcctgagcgc ttcgtcatcg cggcgacgcc gggcagcgcg
     1861 tagaacgcgc gcatgatgcc cgcggcgttg cgcaggtcgc cgatggtttc gaacagcggc
     1921 gagacgatca ggtcggacac ggcgccgtcg tccagcgtgc cgcgcagcag cccgacctct
     1981 ttcagcagca gcatcacctc gagcaggtcg ctcacgtcct cggtgtgcga gatgatgtag
     2041 tggcgcagcg cctggcggcc gtagcgctgc agcatcacga ccgcggcctc gaagatcgcg
     2101 agctcgccgc tcgtgtgctc gctgtagctc gccgcgcgca cgcgcagcgg ccgggcgtcg
     2161 ttgagctgct gcagcagcag cgtgcggcgc gcgtcttcgg cgagcgcgcc gtagtcggcc
     2221 tcgaccttcg cgacgcgcag cagctcggcg acgaccgcct cgtgcttgtc cgaactctgg
     2281 cgcaggtcga ccgtcgcgag atggaagccg aacacctgca ccgcgcgcat cagcggctg
//
LOCUS       DATMJB010000375         2282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_548727, whole genome shotgun sequence.
ACCESSION   DATMJB010000375 DATMJB010000000
VERSION     DATMJB010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548727"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..876)
                     /locus_tag="VNU48_08125"
     CDS             complement(67..876)
                     /locus_tag="VNU48_08125"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HWI11279.1"
                     /translation="MTAASTAVQQLRALAPRAQPRIAIVLGSGWRSLTEHVRDALRIR
                     YAELPGFPQATVLGHSGELWLGRIGAHEVAVMSGRKHAYENGDVKGMQLPLQTLRALG
                     CEVLVQTNAAGSLRAEMPPGSLMVLTDHMNPSQRSPLVGAGGSERFVSMQDAYDPDLR
                     ATAHAVAARQGVELHEGVYLWTLGPQFETPAEVRMFRHWGADAVGMSTVPETILARHA
                     GMRVLALSLLTNMAAGLSEESLSHAHTLSQALAAGDVVGSVLADIVAAIEL"
     gene            complement(873..1283)
                     /locus_tag="VNU48_08130"
     CDS             complement(873..1283)
                     /locus_tag="VNU48_08130"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HWI11280.1"
                     /translation="MLSEPEQDQLLRAARTAQTQAYAPYSHFAVGATVLDEHGRIHAG
                     CNVENAAYPEGLCAEAGAFSAMVLAGGRQVRALAVVGDGAGLVTPCGGCRQKLREFAA
                     PDTPVLVADRTQLRARFTLAQLLPDSFGPEQLAP"
     gene            complement(1302..>2282)
                     /locus_tag="VNU48_08135"
     CDS             complement(1302..>2282)
                     /locus_tag="VNU48_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI11281.1"
                     /translation="MDEALIGALIASTLLVATPLILCALAGLLAERSGVVDIGLEGKM
                     LFAAFAAAASGAVVDSTLLALGLAMAVAVVLSWLHGFACVSHRGDQVVSGMAIDIIAA
                     GLTVVLGIAWFHQGGQTPPVADSVRLHALWPGAQATLERVPVLGAFLGPALGEGLLGH
                     NALVYAALSLVGAVWWLVYRTRFGLRLRAVGENPQMVDAAGVSVLKLRYAALTLNGML
                     CGLAGGYLVLAQNASFAPNMTAGRGFMALAALIFGHWRPLPALLACLLFGFLDAAAIR
                     LQGVSLPGIGEVPVQAIQALPYLLTVILLAGFIGRSNAPKALGIPYVKER"
BASE COUNT          351 a          821 c          827 g          283 t
ORIGIN      
        1 aggtccaggc aggccagcgc gagccgggca ctgtgttgcg ggtcgcgttc agccgtcatc
       61 agagtctcac agttcgatgg ccgcgacgat gtcggcgagc acgctgccca ccacgtcgcc
      121 ggccgcaagc gcctgcgaca aggtgtgcgc gtggctcagc gattcctcgg acaggccggc
      181 ggccatgttc gtcagcagcg acagcgcgag cacgcgcatg ccggcgtggc gcgccaggat
      241 ggtctcgggt accgtgctca tgccgaccgc gtcggcgccc cagtgccgga acatgcggac
      301 ttcggccggg gtctcgaact gcggccccag cgtccacagg tacacgccct cgtgcagctc
      361 gacgccctgg cgcgccgcga ccgcgtgggc ggtggcgcgc aggtcggggt cgtacgcgtc
      421 ctgcatgctg acgaagcgct ccgagccgcc cgcgccgacg agcggcgagc gctgcgacgg
      481 gttcatgtgg tcggtcagca ccatcaggct gccgggcggc atctcggcgc gcaggctgcc
      541 ggccgcgttg gtctgcacca gcacctcgca gccgagcgcg cgcaaggtct gcagcggcag
      601 ctgcatgccc ttcacgtcgc cgttctcgta ggcgtgcttg cgcccgctca tcaccgcgac
      661 ctcgtgcgcg ccgatgcggc cgagccagag ttcgcccgag tggccgagca cggtggcctg
      721 cgggaagccc ggcagctcgg cgtagcggat gcgcagcgcg tcgcgcacgt gctcggtcag
      781 gctgcgccag ccggagccga gcacgatcgc gatgcgcggc tgcgcccgcg gcgcgagcgc
      841 gcgcagctgc tgcacggcgg ttgaagcggc cgtcatggcg cgagctgctc cgggccgaag
      901 ctgtcgggca gcagctgggc gagcgtgaag cgcgcgcgca gttgcgtgcg gtcggcgacc
      961 agcaccggcg tgtcgggcgc agcgaactcg cgcagcttct ggcggcagcc gccgcagggt
     1021 gtcacgagcc cggcgccatc gccgacgacg gcgagtgcgc gcacctgccg gccgccggcc
     1081 agcaccatcg cgctgaacgc gccggcctcg gcgcacaggc cctccgggta ggccgcgttc
     1141 tcgacattgc agccggcatg gatgcggccg tgttcgtcga gcacggtggc gccgaccgcg
     1201 aagtgcgaat acggcgcgta ggcctgcgtc tgcgccgtgc gcgcggcacg cagcagttgg
     1261 tcttgttcgg gttcgctcag catcgttgaa ggatcgcgcg ctcagcgctc cttgacatag
     1321 ggaattccga gcgccttcgg cgcattgctg cggccgatga agccggccag caggatcacc
     1381 gtcagcagat agggcaaggc ctggatcgcc tgcaccggca cctcgccgat gcccggaagg
     1441 ctgacgccct gcaggcggat cgccgcggcg tcgagaaagc cgaacagcag gcacgcgagc
     1501 agggcaggca gcggccgcca gtggccgaag atcagcgcgg ccagcgccat gaagccgcgg
     1561 cctgccgtca tgttcggtgc gaacgaggcg ttctgcgcca gcaccaggta gccgccggcc
     1621 aggccgcaca gcatgccgtt cagcgtcagc gccgcatagc gcagcttcag caccgagacg
     1681 ccggccgcat cgaccatctg cgggttctcg ccgaccgcac gcagccgcag gccgaagcgc
     1741 gtgcgataga ccagccacca gaccgcgccg accagcgaga gcgccgcgta gaccagcgcg
     1801 ttgtggccga gcagcccttc gccgagcgcc ggtccgagaa aggcgccgag caccggcacg
     1861 cgctcgagcg tggcctgcgc gccgggccag agcgcgtgca ggcgcaccga gtcggccacc
     1921 ggcggcgtct gcccaccctg gtgaaaccag gcgatgccca gcaccacggt caggccggcg
     1981 gcgatgatgt cgatcgccat gcccgagacg acctggtcgc cgcggtggct gacgcacgca
     2041 aagccatgca gccacgacag caccaccgcg accgccatcg ccaggccgag cgcgagcagg
     2101 gtcgagtcga ccacggcgcc gctcgccgcg gcggcgaacg cggcgaacag catcttgcct
     2161 tccagaccga tgtcgaccac gcccgagcgc tcggcgagca gcccggcgag cgcgcacagg
     2221 atcagcggtg tcgcgacgag cagggtcgag gcgatcagcg cgccgatcag ggcctcatcc
     2281 at
//
LOCUS       DATMJB010000376         8653 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_422383, whole genome shotgun sequence.
ACCESSION   DATMJB010000376 DATMJB010000000
VERSION     DATMJB010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422383"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..927
                     /locus_tag="VNU48_08140"
     CDS             148..927
                     /locus_tag="VNU48_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI11282.1"
                     /translation="MTPFLSLSVAMGLLTLAWTTHPLWLRSAAPTRFDATAPVPLPGR
                     MPATIAALAALVLAVVGVGYAWVGAPQHLAVAPRPDGHRSALTNTPADPALMQAGSRV
                     AAMIASLAERLAAHPDDADGWHTLARSYAAIGRHAAAVDAFRKAVRLRPDDPSLLADC
                     AVSAALLDPHPAAGAAAQLVARALLLDPANPKALALAGTLALQRKDYAAAIDHWERLA
                     RIEVPGSPIARQLDVSLQQARRMAGIQGTSPAAANEVIKRR"
     gene            complement(958..2238)
                     /locus_tag="VNU48_08145"
     CDS             complement(958..2238)
                     /locus_tag="VNU48_08145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWI11283.1"
                     /translation="MSLKQDLRSVWVALRRGSPVVVDVPPAIRFVLLQRYVDMSMRNG
                     RLSLASFGILIFGLGFEAPLLPRILAWAVLGAVFAARVVLARRMLRRLDPLDPRSDWR
                     YDALVALASSVWGVAPIAFHGWVSPLSEYCITYGAFVATSLLAITYIAALPASLVMVA
                     ASVLPLIAFMALQGPAVMVVLAFGTLVCAVALQLRVFSIHGTLLQALAAERQNAALVQ
                     ELQSYRHALETENASLDSSLRAAAQAANRDPLTGLFNRRHIFAFAESLAARVRSGEEE
                     VTLCMIDVDHFKQVNDRHGHPVGDEVLRAVGALLGARLREGDCLARIGGEEFMIVLRQ
                     CGASRGRRAAESVRHNVAASQIRTAAGEVPITVSMGVAQWAADEDFEAAVRRADRALY
                     DAKHSGRDRVEIDGNDARRVSTIPMDFLPSGPVH"
     gene            2376..3365
                     /locus_tag="VNU48_08150"
     CDS             2376..3365
                     /locus_tag="VNU48_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI11284.1"
                     /translation="MPPPSPLLAVTDLRVTLNTARGPADALRGIDLRLERGATLGLIG
                     ESGCGKSMTAMAIMGLLPPGAAVSGSIVLNGQELVGLPDDAMCALRGDRIGMVFQEPM
                     TALNPLHTVGDQVGEPLRLHRGMTRAAARAEALRLLERVRLPQAAQRLDAYPHQLSGG
                     QRQRVTIAMALACGPDLLIADEPTTALDVTIQAEILTLIRALVEESGMALLMISHDLG
                     VMARTVQRLLVMYGGTVVESGPTGEVFAQLAHPYTRGLFAARPRLGAARVDGRKPALA
                     TIPGRVPELVDMPAGCPFADRCAWVVEACRAAPPAPVVVGPGHAARCIRVGER"
     gene            3362..4153
                     /locus_tag="VNU48_08155"
     CDS             3362..4153
                     /locus_tag="VNU48_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI11285.1"
                     /translation="MSEPLLEVRDLAKHYKLPRERLLQPAPVVEALRGVSFTLHAGRS
                     LGIVGESGSGKSTLARLVMALEAPSAGSVRLLGRDLHALPPTELRDARRDFQMVFQDP
                     YGSLDPRQRVARIVAEPLIGVDAADARRRVGEALDAVGLRASDAAKYPHEFSGGQRQR
                     IAIARALITRPRLIVADEPVSALDVSVQAQVLNLLAQLQQQFGVTYLFISHDLAVIDH
                     VCDEVAVMRAGRIVEHGSTGQVLQQPAHEYTRALLAAIPGVGAFR"
     gene            4392..5909
                     /locus_tag="VNU48_08160"
     CDS             4392..5909
                     /locus_tag="VNU48_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI11286.1"
                     /translation="MKPQPRLGTRALATAAAAALLACAAFAPDALAQARKDSAVLGMV
                     LEPAPGLDPTSAAAAAIGEVVHYNILEGLTKINMDGSVAPLLAESWTIDPDGKSYTFK
                     LRRGVTFHDGALFDASDVKFSFERAKAPASTNKAKKAVFDNISRIDTPDPFTVIVVLN
                     QPDGNFLFRMGENTAVILDPKSAATTATRPVGTGPFKLDTWAKGASITLSRNDAYRNP
                     AAIKLKKVTLRFISDPAAQVAALLAGDIDGMPRFGAIQSLKQFQGDARFAVVAGGTEG
                     KTIVSINNRKKPFDDVRVRRALAAAIDRKAVIDGSSEGYGKAIGSHMVPSDAGYIDLT
                     AANPYDPEKAKALLKEAGVATPLSVTLTLPPPEYARKGGEIVAAQLAKVGINAKIENV
                     EWAQWLSGTFKGNYDLTIVSHVEPLDFDRYADPAYYWGYNSKAYNDLLAKYNGTTDAK
                     GRLKLLGDIQRQLATDSVNVYLYQLPQFAVGKKQLKGMWSSSPVFANDLSALSWQ"
     gene            5906..7306
                     /locus_tag="VNU48_08165"
     CDS             5906..7306
                     /locus_tag="VNU48_08165"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWI11287.1"
                     /translation="MTALHDLGAVELLAAYRAKALSPVDVTRALLAHIDAWEPQLHAT
                     YALDADAALAQAEASQARWLKGAPRGALDGVPVTIKENIATQGVPVPLGCAATELVPA
                     ERDAPPAARLREAGAVIVAKTTMPDYGMLSSGLSSFHALTRNPWDLSKGPGGSSAGAA
                     AGAAAGYGPLHVGTDIGGSIRLPAGWCGVFGLKPSLGRIPIFPPYAGRVAGPMTRTVT
                     DAALLMATLSLPDARDTMSLPYQPIEWQHLERDLKGLRIGLQLDAGWGLAVDPEVRAA
                     VEAAAHLFAAAGAIVTPVPPFMTRAMIDGMDDFWRTRSWLEIGALPAARRAKVLPYIT
                     EWARGGEGLSGERVFRGYSMMGAMREAAVQACAPFDMLLSPTAPIAAFPAEFAGPTND
                     PATPFEHIAFTLPFNMSEQPAASINCGYTTGGLPVGLQIVGRRFDDLGVLQVARAFER
                     LRPAQRPWPLPPPRTA"
     gene            7317..8651
                     /locus_tag="VNU48_08170"
     CDS             7317..8651
                     /locus_tag="VNU48_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI11288.1"
                     /translation="MFDPRGIAVSTRSVDAVASCERALWRMMSFYGTPIDDLAAAHAA
                     DPGWPLPLLMHAGFLLSLTEPSVVPGARALLDRAEPMIARANHRERDHLVALHTLLAG
                     DWHGACREWEALLLQHPRDALALQWAHLFDFYRGDATNLRQRVARVLPEWDARDALHP
                     YVLGLYAFGLEECQLYAQAEETGRRALAGAAKVPWAIHAVAHVMEMQGRHTEGAAWML
                     QWHGDWAEGNGFSGHLGWHHALFALEALDSAEALRLFDAHLHADPAQITLQRLDASSL
                     LWRLHLLGADVGTRWARLVEGWDLSDQSAGTFPFNDLHALLALIGAGELARAQAWTQQ
                     VATRAERSGGSNRAIARDVGGPLMRGMLAFAHGRFDVAIETLYPVRALASQFGGSHAQ
                     RDLIDQTLLAAAARAVDRSAGRALLNERRLGKPETPLTAHWAGRLGLRRAAR"
BASE COUNT         1236 a         3152 c         3123 g         1142 t
ORIGIN      
        1 ggcgagcacg ctggcgctgt ggctggcgcc gggcctgctg ctcgcgggcg gcctggcgat
       61 gctggggtcg gtgctgcgcc ggcgcgcgcg cctgagcgac gcgcatttcg aacccgatcg
      121 atctgacgcg ccggaagggg cgacgtcatg acgccgttcc tgtcgctgag cgtcgcgatg
      181 ggcctgctga cgctcgcgtg gacgacgcac ccgctgtggc tgcgcagcgc cgcaccgacg
      241 cgcttcgacg cgaccgcgcc ggtcccgttg cccggccgca tgccggcgac catcgccgcg
      301 ctggcggcgc tcgtgctggc ggtggtcggc gtcggctacg cgtgggtcgg tgcgccgcag
      361 catctggccg tggcgccgcg acctgatggg catcgcagcg cgctgacaaa cacacctgcc
      421 gaccctgcgc tgatgcaggc cgggtcgcgc gtcgccgcga tgatcgcgag cctcgccgag
      481 cggctcgcgg cgcaccccga cgatgccgac ggctggcaca cgctggcccg ctcgtacgcc
      541 gccatcgggc gccacgccgc ggcggtcgac gcgttcagga aggccgtgcg gctgcgcccc
      601 gacgacccga gcctgctcgc cgactgcgca gtctcggccg cgctgctgga cccgcacccc
      661 gccgccggtg cggccgcaca gctggtcgca cgtgcgctgc tgctcgaccc ggcgaatccg
      721 aaggcgctgg cgctggccgg cacgctggcg ctgcagcgca aggactatgc ggcggcgatc
      781 gaccactggg agcggctggc gcgcatcgaa gtgcccggca gcccgatcgc gaggcagctt
      841 gacgtcagcc tgcagcaggc gcgccggatg gccggcatcc agggcacgtc gccggcggcc
      901 gcgaacgagg tcatcaagcg gcgttgaacc ggcgcccgcg cgggcggccg cagcgactca
      961 atgcaccggc ccggacggca ggaaatccat cgggatcgtc gacacgcgcc gcgcgtcgtt
     1021 gccgtcgatc tcgacccggt cgcggccgct gtgcttggcg tcgtacaggg cccggtcggc
     1081 gcgccggacc gcggcctcga aatcttcatc ggcggcccat tgcgccacac ccatcgacac
     1141 cgtgatcggc acctcgcccg cggcggtgcg gatctgcgac gcggcgacgt tgtggcgcac
     1201 cgattcggca gcgcgccggc cgcggctcgc gccgcactgg cgcagcacga tcatgaactc
     1261 ctcgccgccg atgcgcgcca ggcagtcgcc ctcgcgcagc ctcgcgccga gcagcgcgcc
     1321 gacagcgcgc agcacctcgt cgccgaccgg gtggccgtgg cggtcgttga cctgcttgaa
     1381 gtgatcgacg tcgatcatgc acagcgtcac ttcctcttcg cccgagcgca cccgcgcggc
     1441 cagcgattcg gcgaacgcga agatgtggcg gcggttgaac aggccggtca gcgggtcgcg
     1501 gttggcggcc tgcgcggcgg cgcgcagcga gctgtcgagc gaggcgttct cggtctccag
     1561 cgcgtgccgg tagctctgca gctcctgcac cagcgcggcg ttctggcgct cggccgcgag
     1621 cgcctgcaac agcgtgccgt ggatgctgaa cacgcgcagc tgcagcgcga ccgcgcagac
     1681 cagcgtaccg aacgccagca ccaccatcac cgccgggccc tgcagcgcca tgaaggcgat
     1741 cagcggcagc acgctggcag cgaccatcac caggctcgcc ggcagcgccg cgatgtaggt
     1801 gatcgcgagc agcgaggtcg cgacgaacgc gccgtaggtg atgcagtact cgctgagcgg
     1861 cgagacccag ccgtggaacg cgatcggcgc cacgccccag accgacgagg cgagcgccac
     1921 gagcgcgtcg tagcgccagt cggagcgcgg gtcgagcggg tcgagtcgcc gcagcatgcg
     1981 gcgcgcaagc acgacgcgcg ccgcgaacac cgccccgagc acggcccacg cgaggatccg
     2041 cggcagcagc ggggcctcga atccgaggcc gaagatcagg atgccgaacg acgcgagcga
     2101 gagccggccg ttgcgcatcg acatgtcgac gtagcgctgc agcagcacga agcggatcgc
     2161 cggcggcacg tcgacgacca ccggcgagcc gcggcgcagc gcaacccaga ccgaacggag
     2221 atcctgcttc aacgacatcg cggaatgttc ttcttcggcg tgcgggcggc acgcgccgac
     2281 acggcgcgca gcccccgcgc gcggcggcca ccgatgctac cgtggcgcgc cacccgcggc
     2341 cgcgcgtcgc gagcggcgcg cggcacaatg acacagtgcc gcccccctcc ccgctgcttg
     2401 ccgtcaccga tctgcgggtc acgctgaaca ccgcgcgcgg cccggcggat gcgctgcgcg
     2461 gcatcgacct gcggctcgaa cgcggcgcca cgctcggcct gatcggcgag agcggctgcg
     2521 gcaaatcgat gacggcgatg gcgatcatgg ggctgctgcc gcccggcgcc gcggtcagcg
     2581 gcagcatcgt gctgaacggc caggagctcg tcggcctgcc cgacgacgcg atgtgcgcgc
     2641 tgcgcggcga ccgcatcggc atggtgttcc aggagccgat gaccgcgctg aaccccttgc
     2701 acaccgtcgg cgaccaggtc ggcgaaccgc tgcgcctgca ccgcggcatg acgcgcgccg
     2761 cggcccgcgc cgaggcgctg cgcctgctcg agcgggtgcg gttgccgcag gccgcgcagc
     2821 ggctcgatgc ttacccgcac cagctctcgg gcggccagcg ccagcgcgtc acgatcgcga
     2881 tggcgctggc ctgcggcccc gacctgctga tcgccgacga gccgacgacc gcgctcgacg
     2941 tgacgatcca ggccgagatc ctgacgctga tccgcgcgct cgtcgaggaa tcgggcatgg
     3001 cgctgctgat gatcagccac gacctgggcg tgatggcgcg cacggtgcag cggctgctcg
     3061 tgatgtacgg cggcacggtg gtcgagtcgg ggccgaccgg cgaggtgttc gcacagctcg
     3121 cgcaccccta cacgcgcggc ctgttcgcgg cccggccgcg gctcggcgcg gcgcgcgtgg
     3181 acggccgcaa gccggcgctc gcgacgattc cggggcgcgt gcccgagctg gtcgacatgc
     3241 cggccggctg cccgttcgcc gaccgctgcg cgtgggtcgt cgaggcctgc cgcgccgcgc
     3301 cgccggcgcc ggtggtcgtc gggccgggac acgccgcgcg ctgcatccgc gtcggtgagc
     3361 gatgagcgag ccgctgctgg aggtgcgcga cctcgccaag cactacaagc tgccgcgcga
     3421 gcgcctgctg cagccggcgc cggtggtcga ggcgttgcgc ggcgtgagct tcacgctgca
     3481 tgccgggcgc agcctgggca tcgtcggcga atcgggctcg ggcaagtcga cgctggcgcg
     3541 gctggtgatg gcgctcgagg cgcccagtgc cggcagcgtg cggctgctcg gccgcgacct
     3601 gcacgcgctg ccgcccaccg agttgcgcga cgcgcggcgc gacttccaga tggtgttcca
     3661 ggacccgtac ggttcgctcg acccgcgcca gcgcgtcgcg cgcatcgtcg ccgagccgct
     3721 gatcggcgtc gacgcggccg acgcgcgccg gcgcgtcggc gaggcgctcg acgcggtcgg
     3781 cctgcgcgcc agcgacgcgg ccaagtaccc gcacgaattc tccggcggcc agcgccagcg
     3841 catcgcgatc gcgcgcgcgc tgatcacgcg gccgcggctg atcgtcgccg acgaaccggt
     3901 cagcgcgctc gacgtgtcgg tgcaggcgca ggtgctgaac ctgctcgcac agctgcagca
     3961 gcagttcggc gtgacctacc tgttcatcag ccacgacctc gcggtgatcg accacgtgtg
     4021 cgacgaggtc gcggtgatgc gcgccgggcg catcgtcgag cacggctcga ccgggcaggt
     4081 gctgcagcag ccggcgcacg agtacacgcg ggccttgctc gccgcgatcc ccggcgtcgg
     4141 tgcgttccgc tagtgcagtg ttcgacacta tgcagcacat aaaaccgcgt ccttgccgcc
     4201 ctggcgtcgt tgcaaatcct cgcgatacct tcgggtatcc ctgcggtttg cgcctaacca
     4261 ggacgacaaa tccatcggtt tttttaagtg ctgcacagtg ccgaacactg cactagagtc
     4321 ggaacccgcg ccgcgggccg ccgtccggct tgcacacggg cgcatccgaa gaacacctca
     4381 ggagtgagcc gatgaagccg caaccccgac tcggcacccg cgcgctggcg accgccgccg
     4441 ctgccgcgct gctggcctgc gccgccttcg cgcccgacgc gctggcccag gcgcgcaagg
     4501 acagtgccgt gctcggcatg gtgctcgagc ccgcgccggg cctggacccg accagcgcgg
     4561 cggcggccgc gatcggcgag gtcgtgcact acaacatcct cgaaggcctg accaagatca
     4621 acatggacgg cagcgtggcg ccgctcctgg ccgagagctg gaccatcgac cccgacggca
     4681 agagctacac cttcaagctg cgccgcggcg tgacgttcca cgacggcgca ctcttcgacg
     4741 cgagcgacgt gaagttcagc ttcgagcgcg ccaaggcgcc ggcctcgacc aacaaggcca
     4801 agaaggcggt gttcgacaac atcagccgca tcgacacgcc cgacccgttc accgtgatcg
     4861 tggtgctgaa ccagccggac ggcaacttcc tgttccgcat gggcgagaac accgcggtga
     4921 tcctcgaccc gaagagcgcg gcgaccacgg cgaccaggcc ggtgggcacc ggcccgttca
     4981 agctcgacac gtgggcgaag ggcgcgtcga tcacgctgag ccgcaacgac gcttaccgca
     5041 accccgcggc gatcaagctg aagaaggtca cgctccgctt catcagcgac ccggcggcgc
     5101 aagtcgcggc cttgctggcc ggtgacatcg acggcatgcc gcgcttcggc gcgatccaga
     5161 gcctgaagca gttccagggc gatgcgcgct tcgcggtcgt ggccggcggc accgagggca
     5221 agaccatcgt cagcatcaac aataggaaga agccgttcga cgacgtgcgc gtgcgccgcg
     5281 cgctggccgc cgcgatcgac cgcaaggcgg tgatcgacgg ctcgtccgag ggctacggca
     5341 aggcgatcgg cagccacatg gtgccgagcg acgccggcta catcgacctg accgcagcga
     5401 acccgtatga ccccgagaag gcgaaggcgc tgctgaagga ggccggcgtc gccacgccgc
     5461 tgagcgtgac cctgaccctg ccgccgccgg agtacgcgcg caagggcggc gagatcgtcg
     5521 cggcgcagct ggccaaggtc ggcatcaatg cgaagatcga gaacgtcgag tgggcgcagt
     5581 ggctgtcggg caccttcaag ggcaactacg acctgaccat cgtcagccat gtcgaaccgc
     5641 tggacttcga ccgctacgcc gacccggcct actactgggg ctacaactcg aaggcctaca
     5701 acgacttgct cgcgaagtac aacggcacga ccgatgcgaa ggggcggctg aagttgctcg
     5761 gtgacatcca gcgccagctc gcgaccgatt cggtcaacgt ctacctgtac cagctgccgc
     5821 agttcgcggt cggcaagaag cagctgaagg gcatgtggag cagctcgccg gtcttcgcga
     5881 acgacctgtc ggcgctctcc tggcaatgac ggcgctgcac gatctgggcg cggtcgagtt
     5941 gctcgcggcg taccgcgcga aggcgctgtc gccggtcgac gtcacgcgcg cgctgctcgc
     6001 gcacatcgac gcctgggagc cgcagctgca cgcgacctac gcgctcgacg ccgacgccgc
     6061 gctggcgcaa gccgaggcct cgcaggcgcg ctggctgaaa ggcgcgccgc gcggggcgct
     6121 cgacggcgtg ccggtgacga tcaaggagaa catcgcgacg cagggcgtgc cggtgccgct
     6181 gggctgcgcg gcgaccgagc tcgtgccggc cgagcgcgat gcgccgccgg cggcgcgcct
     6241 gcgcgaggcc ggcgcggtga tcgtcgcgaa gaccacgatg ccggactacg ggatgctgtc
     6301 gtccggcctg tcgagctttc acgcgctgac gcgcaacccc tgggacctga gcaagggccc
     6361 cggtggcagc agcgccggcg cggcggcggg cgcggcggcg ggctacggcc cgctgcatgt
     6421 cggcaccgac atcggtggct cgattcggct gccggccggc tggtgcggcg tcttcggcct
     6481 gaagcccagc ctcgggcgca tcccgatctt cccgccgtac gcgggccggg tggccgggcc
     6541 gatgacgcgc acggtcacgg atgcggcgct gctgatggcc acgctgagcc tgcccgacgc
     6601 gcgcgacacg atgagcctgc cgtaccagcc gatcgagtgg cagcacctcg aacgcgacct
     6661 gaaggggctg cgcatcgggc tgcagctgga cgccggctgg ggcctggccg tagaccccga
     6721 agtgcgcgcc gcggtcgagg cggccgcgca tctgttcgcg gcggccggcg cgatcgtcac
     6781 gccggtcccg ccgttcatga cgcgcgcgat gatcgatggc atggacgact tctggcgcac
     6841 gcgctcgtgg ctggagatcg gcgcgctgcc ggcggcgcgg cgcgccaagg tgctgccgta
     6901 catcaccgaa tgggcacgcg gcggcgaggg cttgtcgggc gagcgcgtgt tccgcggcta
     6961 cagcatgatg ggcgcgatgc gcgaggccgc ggtgcaggcc tgcgcgccgt tcgacatgct
     7021 gctgtcgccg accgcaccga ttgccgcgtt cccggccgag ttcgccggcc cgaccaacga
     7081 cccggccacg ccgttcgagc acatcgcgtt cacgctgccg ttcaacatga gcgagcagcc
     7141 ggccgcgagc atcaattgcg gctacacgac gggcggcctg ccggtcgggc tgcagatcgt
     7201 cggccggcgc ttcgacgacc tcggcgtgct gcaggtcgcg cgcgccttcg agcggctgcg
     7261 gcccgcgcag cggccctggc cgctgccgcc gccgcggacg gcctgagcac ggcgccatgt
     7321 tcgatccgcg cggcatcgcc gtcagcaccc gctcggtcga cgcggtggcg tcatgcgaac
     7381 gcgcgctgtg gcggatgatg tcgttctacg gcacgccgat cgacgacctc gccgccgcgc
     7441 acgccgccga cccgggctgg ccgctgccgc tgctgatgca cgccggcttc ctgctgagcc
     7501 tgaccgaacc ttcggtcgtg ccgggggcgc gggcactgct ggaccgcgcc gagccgatga
     7561 tcgcccgcgc gaaccaccgc gagcgcgatc acctggtcgc gctgcacacc ctgctggccg
     7621 gcgactggca cggtgcctgc cgcgaatggg aggccttgct gctgcagcac ccgcgcgatg
     7681 cgctcgcgct gcagtgggcg cacctgttcg acttctaccg cggcgacgcc accaatctgc
     7741 gccagcgcgt cgcgcgcgtg ctgcccgagt gggacgcgcg cgacgcgctg catccctacg
     7801 tgctcggcct gtacgcgttc ggcctggagg aatgccagct ctacgcgcag gccgaagaga
     7861 ccggccggcg cgcgctggcc ggcgccgcga aggtgccgtg ggcgatccac gcggtcgcgc
     7921 acgtgatgga gatgcagggc cgccacaccg agggtgccgc ctggatgttg caatggcacg
     7981 gcgactgggc cgagggcaac ggtttctcgg gtcacctcgg ctggcatcac gcgctgttcg
     8041 cgctcgaggc gctcgactct gccgaggcgc tgcgcctgtt cgatgcgcac ctgcacgccg
     8101 accccgcgca gatcaccttg cagcgtctcg acgcgtcgtc gctgctgtgg cgcctgcacc
     8161 tgctcggcgc cgatgtcggc acgcgctggg cccggctcgt cgagggctgg gacctgtcgg
     8221 accagagcgc cggcacgttc ccgttcaacg acctgcacgc gctgctcgcg ctgatcggcg
     8281 ccggcgagct ggcacgcgcg caagcctgga cgcagcaggt cgcgacgcgt gccgagcgca
     8341 gcggcggcag caaccgcgcg atcgcgcgcg atgtcggcgg cccgttgatg cgcggcatgc
     8401 tcgcgttcgc gcacgggcgc ttcgacgtcg cgatcgagac gctctacccg gtgcgtgcgc
     8461 tggccagcca gttcggcggc agccatgcgc agcgcgacct gatcgaccag accctgctcg
     8521 ccgccgcggc gcgcgcggtc gaccgctcgg cgggccgggc gctgctgaac gagcggcgcc
     8581 tgggcaagcc cgagacgcct ctgaccgcgc actgggccgg tcgcctcggg ctgcgccgcg
     8641 ccgcacgttg aac
//
LOCUS       DATMJB010000377         2443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_771222, whole genome shotgun sequence.
ACCESSION   DATMJB010000377 DATMJB010000000
VERSION     DATMJB010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771222"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VNU48_08175"
     CDS             complement(<1..816)
                     /locus_tag="VNU48_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HWI11289.1"
                     /translation="MTVTTTGIEVRAQSRFVRECIELVSSMRFSISLLTVICIASVIG
                     TVVKQHEPYTNYVNQFGPFWAELFSRVGLFSVYSVWWFLLILAFLVVSTSLCIARNVP
                     KILHDLRTYKEQVREQALASFHHRGQAELAESRDQAFERIGALLVHGGWKAKVQVRAN
                     GTMIAARRGAANKLGYIAAHSAIVLVCVGGLLDGDLIVRAQMALQGKSSYTGGGMMKD
                     VPDRHRLGPDTPTFRANLLVPEGSKSGTAVISMQDGVVLQDLPFDVELKKFIVD"
     gene            complement(911..1540)
                     /locus_tag="VNU48_08180"
     CDS             complement(911..1540)
                     /locus_tag="VNU48_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWI11290.1"
                     /translation="MKSVASALASLLLATCACAALAEEAKAPAPKADLAKGQATSTNV
                     CAACHTNDGSRGSPANPILQGQHPEYLVKQLTEFKAGKRQNAVMSGMVAALSEDDMRN
                     VAAFYAGKQAKPGFAKNKATVALGEKIYRGGIADRSIPACAGCHSPSGAGIPAQYPRL
                     AGQHADYTEAQLVAFRSGTRKNSVPMTGVAAKLNDGEIKAVADYIAGLH"
     gene            1602..2291
                     /gene="yihA"
                     /locus_tag="VNU48_08185"
     CDS             1602..2291
                     /gene="yihA"
                     /locus_tag="VNU48_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HWI11291.1"
                     /translation="MAEQNKSSPAPVALVDEAKRALGWAHTARFRTTASQLDQLPQTE
                     LPEIAFVGRSNAGKSTAINTLTQHKRLAFASKTPGRTQHINLFDLGPKMVPNALFADL
                     PGYGYAAVARGAKLRWQKVMADYLDVRRSLVGVVLMVDSRLGFTDLDQQLMSFVAPRV
                     GNGSVRLLVLLTKSDKLNRAESSAALAAAQTLLGEITTDEADVGVALFSALKKSGVDD
                     AAKVLYGWVHG"
BASE COUNT          374 a          810 c          851 g          408 t
ORIGIN      
        1 gtcgacgatg aacttcttca gctcgacatc gaacggcagg tcttgcagca cgacgccgtc
       61 ctgcatgctg atcaccgccg tcccggattt cgagccctcg ggcaccagca ggttcgcacg
      121 gaacgtcggg gtgtcggggc cgagccgatg gcggtccggc acgtccttca tcatgccgcc
      181 gccggtatag ctgctcttgc cctgcagcgc catctgtgcg cgcacgatca ggtcaccgtc
      241 gagcagcccg ccgacgcaca ccagcacgat cgccgaatgc gccgcgatgt agccgagctt
      301 gttggcggcg ccgcggcgcg cggcgatcat cgtgccgttc gcccgcacct ggaccttcgc
      361 cttccagccg ccgtgcacga gcagcgcgcc gatgcgctcg aaggcctggt cgcgcgattc
      421 ggcgagctcg gcctggccgc ggtggtggaa cgacgccagc gcctgctcgc gcacctgctc
      481 cttgtaggtg cgcaggtcgt ggaggatctt cggcacattg cgcgcgatgc acaggctggt
      541 cgagacgacc aggaacgcca ggatcagcag gaaccaccag acgctgtaga cgctgaacag
      601 gcccacgcgc gagaacagct cggcccagaa cggcccgaac tggttgacgt agttggtgta
      661 gggctcgtgc tgcttgacga ccgtgccgat caccgaggcg atgcagatca ccgtcagcag
      721 cgagatcgaa aagcgcatcg acgacaccag ctcgatgcac tcgcgcacga agcgcgactg
      781 cgcgcgcact tcgatgccgg tggtggtgac ggtcatgggc ggattatccg gggccgggcg
      841 cccgagcgtc ctgctccaac gcaaagggcc ggcgcaagac cggcccttgg gcgacgcggc
      901 gccgattcgt tcagtgcagg ccggcgatgt agtcggccac ggccttgatc tcgccgtcgt
      961 tcagcttggc ggccacgccc gtcatcggca cgctgttctt gcgcgtgccg ctgcggaacg
     1021 cgacgagctg tgcctcggtg tagtcggcgt gctggccggc caggcgcggg tactgggccg
     1081 ggatgccggc accgctcggg ctgtggcagc cggcgcaggc cgggatgctg cggtcggcga
     1141 tgccgccgcg gtagatcttc tcgcccagcg cgaccgtggc cttgttcttc gcgaagccgg
     1201 gcttggcctg cttgccggcg tagaacgcgg cgacgttgcg catgtcgtct tcgctgagcg
     1261 cggcgaccat gcccgacatc accgcgttct ggcgcttgcc cgccttgaac tcggtcagct
     1321 gcttgaccag gtactccggg tgctggcctt gcaggatcgg gttggccggg ctgccgcgcg
     1381 aaccgtcgtt cgtgtggcag gccgcgcaca cgttggtcga cgtggcctgg cctttggcca
     1441 gatcggcctt cggggcaggg gctttggctt cttccgcgag ggctgcgcag gcgcaggtgg
     1501 cgagcagcag ggaggccagg gcagaggcaa cagacttcat gagcgcagca gtttcttgtg
     1561 caaaacccgg tgattttaca atgacggctt tgggcgactc catggccgag caaaacaaat
     1621 ccagtccggc gcccgttgcc ctcgtcgacg aggccaagcg cgcgctcggc tgggcgcaca
     1681 ccgcgcgctt tcgcacgacg gccagccagc tcgatcagct gcctcagacc gaactgcccg
     1741 agatcgcctt cgtcggccgc tcgaacgcgg gcaagtcgac cgcgatcaac acgctgacgc
     1801 agcacaagcg cctggcgttc gcgtcgaaga cgccagggcg cactcagcac atcaacctgt
     1861 tcgacctcgg gccgaagatg gtgccgaacg cgctgttcgc cgacctgccg ggttacggct
     1921 atgcggcggt ggcgcgcggc gcgaagctgc gctggcagaa ggtgatggcc gactacctcg
     1981 atgtgcggcg cagcctggtc ggcgtggtgc tgatggtgga ttcgcgcctg ggcttcaccg
     2041 atctggatca gcagctgatg agcttcgtcg cgccgcgcgt cggcaatggc tccgtgaggc
     2101 tgctggtgct gctgaccaag tccgacaagc tgaaccgcgc cgagtccagc gcggcgttgg
     2161 ccgctgcgca gacgctgctc ggcgagatca cgaccgacga ggccgatgtc ggcgtggcgc
     2221 tgttctcggc gctgaagaag tccggcgtcg acgacgcggc caaggtgttg tacggctggg
     2281 tccacggctg agcatgttgt gtcccgcggg tgacgtgaca cccgcgtcga tcggccgata
     2341 ctggctgccc gaggagacga cgatgattca gaccttcgac gcggcgctgc cgcacggcat
     2401 cacgctgagc tgccgcgcca ccggtcgccc cggcgcgccg ctg
//
LOCUS       DATMJB010000378         2006 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_238455, whole genome shotgun sequence.
ACCESSION   DATMJB010000378 DATMJB010000000
VERSION     DATMJB010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238455"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /gene="oxc"
                     /locus_tag="VNU48_08190"
     CDS             <1..1456
                     /gene="oxc"
                     /locus_tag="VNU48_08190"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HWI11292.1"
                     /translation="FLNGLTALAHATTNCYPMILISGSSEREIVDLQQGDYEEMDQLA
                     IAKPLCKAAFRVLHAQDIGVGIARAIRSALSGRPGGVYLDLPAKLFAQTMDAVAGRNS
                     LIKVVDPAPRQIPAPDSVERALAMLKGAKRPLILLGKGAAYAQADADIRALVEKTGIP
                     YLPMSMAKGLLPDTHAQSAAPARSYVLAEADVVMLVGARLNWLLSHGKGKTWGARPKQ
                     FIQIDIAPTEIDSNVAIAAPLIGDIGSCVSALLSGIGPNWARPPADWLVAIAERRDKN
                     LSKMAETLAKNPSPMNFHSALNVVRELVKKHPDAMLVNEGANTLDFTRSIVDMYEPRK
                     RLDVGTWGIMGIGMGFAVAAAVTTGKPVIAIEGDSAFGFSGMEVETICRYNLPVCIVV
                     FNNNGVYKGTDQNPRGGDVAPTVFVKNARYDLMMQAFGGVGVHATTPAQLAAAMAEAI
                     ASGKPTLINAVIDETAGTESGRITSLNPQAAVKK"
     gene            1500..>2006
                     /locus_tag="VNU48_08195"
     CDS             1500..>2006
                     /locus_tag="VNU48_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWI11293.1"
                     /translation="MSTKPLEGIKIIDFTHVQAGPACTQMLAWFGADVIKVERPGSGD
                     VTRSQLRDMPNVDALYFTMLNSNKRSLTLDTKKAEGKAVLEKLIKESDVMVENFGPGA
                     LDRMGFTWEYIQKLNPGMILASVKGFSEGHHYEDLKVYENVAQCAGGAASTTGWWKGE
                     NSAPTISAA"
BASE COUNT          377 a          684 c          660 g          285 t
ORIGIN      
        1 cttcctcaac ggcctgaccg cgctcgccca cgccaccacc aactgctacc cgatgatcct
       61 gatctcgggc tcgtccgagc gcgagatcgt cgacctccag cagggcgact acgaagagat
      121 ggaccagctc gcgatcgcca aaccgctgtg caaggcggcg ttccgggtgc tgcatgcgca
      181 ggacatcggc gtgggcattg cgcgcgcgat ccgttcggcg ctgtccgggc gcccgggcgg
      241 cgtctacctc gacctgcccg ccaagctgtt cgcgcagacc atggacgccg tggcgggcag
      301 gaactcgctg atcaaggtcg tcgatccggc gccgcgccag atcccggcac cggactcggt
      361 cgagcgtgcg ctggcgatgc tcaaaggcgc caagcgcccg ctgatcctgc tcggcaaggg
      421 cgcggcctac gcgcaggccg atgccgacat tcgcgcgctg gtcgagaaga ccggcattcc
      481 ctacctgccg atgtcgatgg ccaagggcct gctgcccgac acccacgcgc aatcggcggc
      541 accggcgcgc tcgtacgtgc tggcggaggc cgatgtcgtg atgctggtcg gtgcgcgcct
      601 gaactggctg ctgtcgcacg gcaagggcaa gacctggggc gccaggccga agcagttcat
      661 ccagatcgac atcgcgccga ccgagatcga cagcaacgtg gcgatcgccg cgccgctgat
      721 cggcgacatc ggctcgtgcg tgtcggcgct gctgagcggc atcggtccga actgggcccg
      781 gccgccggcc gactggctag tcgcgatcgc cgagcgccgc gacaagaacc tgtcgaagat
      841 ggccgagacg ctggcgaaga acccgtcgcc gatgaacttt cacagcgcgc tgaacgtggt
      901 gcgcgagctc gtgaagaagc acccggacgc gatgctcgtc aacgaaggcg ccaacacgct
      961 ggacttcacg cgcagcatcg tcgacatgta cgagccgcgc aaacgcctgg acgtcggtac
     1021 ctggggcatc atgggcatcg gcatgggctt cgcggtggcc gcggcggtga cgaccggcaa
     1081 gcccgtgatc gcgatcgagg gcgacagcgc cttcggcttc agcggcatgg aggtcgagac
     1141 gatctgccgc tacaacctgc cggtctgcat cgtcgtcttc aacaacaacg gtgtctacaa
     1201 gggcaccgac cagaacccgc gtggcggcga tgtggcgccc acggtgttcg tcaagaacgc
     1261 ccgctacgac ctgatgatgc aggccttcgg cggggtgggc gtccatgcga cgacgccggc
     1321 gcagctggcg gcggcgatgg ccgaagccat cgcgtctggg aagccgacgc tgatcaacgc
     1381 ggtcatcgac gagaccgccg gcaccgagag cggccgcatc accagcctga accctcaggc
     1441 cgcggtcaaa aagtaagccc gccgcaacga ttcccctcgc caaaaatacg gagtactcca
     1501 tgagcaccaa acccctcgaa ggcatcaaga tcatcgactt cacgcacgtc caggccggcc
     1561 cggcctgcac gcagatgctg gcctggttcg gcgccgacgt gatcaaggtc gagcgccccg
     1621 gttcgggcga cgtgacgcgc agccagttgc gcgacatgcc caacgtcgac gcgctgtact
     1681 tcacgatgct caacagcaac aagcgctcgc tcacgctgga caccaagaag gccgagggca
     1741 aggcggtgct ggagaagctc atcaaggagt ccgacgtgat ggtcgagaac ttcggccccg
     1801 gcgcgctcga ccggatgggc ttcacctggg agtacatcca gaagctcaac cccggcatga
     1861 tcctggcctc ggtcaagggc ttcagcgaag gccaccacta cgaggacctg aaggtctacg
     1921 aaaacgtggc gcaatgcgcc ggcggcgcgg cctcgaccac cggctggtgg aagggcgaga
     1981 actcggcccc caccatctcg gccgcc
//
LOCUS       DATMJB010000379         6631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_321798, whole genome shotgun sequence.
ACCESSION   DATMJB010000379 DATMJB010000000
VERSION     DATMJB010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6631
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321798"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VNU48_08200"
     CDS             complement(<1..328)
                     /locus_tag="VNU48_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HWI11294.1"
                     /translation="MLAGSVCAQTSGPDEPIELRASPTLHPAPRGDAAHRLPIRLDAQ
                     TVTASPDLDAVAEGNAEFRRGGMKISADRLRYEQAEDLATAVGHVRISRDGNVYSGPE
                     LQLKVQR"
     gene            535..1611
                     /locus_tag="VNU48_08205"
     CDS             535..1611
                     /locus_tag="VNU48_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWI11295.1"
                     /translation="MSTPTPPASADAIAWPDPARRRAFEGWLESIAPAHALQPTSLRA
                     ASSDASFRRYLRVDGAGSSFIVMDAPPPLEDVRPFIQVAGLLHAAGLSAPQVLEQDVP
                     HGFLLLTDLGSHLYLAALHDAVAHGDEARRNALMRDATRALIRLQTQVDARPLPPYDD
                     ALLRRELALFPDWCLTRECGASWSADEQQQWQDACDLLVRSALAQPTVAVHRDYMPRN
                     LMLLGGDANPGILDFQDAVRGPISYDLACLLRDAFISWDEELEIDWAVRYWEAARRAA
                     LPLPDDFGEFWRQVEWMGLQRHLKVLGIFCRLKHRDAKPNYSKDLPRFFNYAHKVAAR
                     YNALRPLSRLLEPLMGAQRVEGFY"
     gene            complement(1628..2626)
                     /locus_tag="VNU48_08210"
     CDS             complement(1628..2626)
                     /locus_tag="VNU48_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWI11296.1"
                     /translation="MDRLHSMRVFSRVVDEGSFARAARELNLSPAVVTRLVADLEEHL
                     GSRLINRTTRRLSLTDTGELYLERVRHILTEVEEAEALATAATSEPRGHLRVLCSPAF
                     AVHQIAKHLPRFRALYPRVSLELSVPGPVETVDENFDVSILTEGRRPLDGNFVARRLA
                     RSEVILCAAPEYLDRRGRPQHPKDMDGHESMVPPFLRELTFHVGAPGDADAAGESFTL
                     SPKRAGLSTIHTDTMYAAALAGLGITGLPSFVAEDALLENALERVLPQWHLFTNTLYA
                     GMPTRKHVPARTRVFVDFLVKTFGGEDRDPWLLAAGCETQPIGKRAPVQASLALAP"
     gene            2668..2802
                     /locus_tag="VNU48_08215"
     CDS             2668..2802
                     /locus_tag="VNU48_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11297.1"
                     /translation="MNHTLFIDASAMPTVQSIDEEDLTEAGQDGNKEQPPRCPERQSH
                     "
     gene            2819..3301
                     /locus_tag="VNU48_08220"
     CDS             2819..3301
                     /locus_tag="VNU48_08220"
                     /inference="COORDINATES: protein motif:HMM:NF025045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4148 domain-containing protein"
                     /protein_id="HWI11298.1"
                     /translation="MNSKFLYAATVAISLISTFAMADEAPATRAQVNAELRQAIVDGT
                     LQRSDYSADSYRLLARTSTKTRAQVGVELAQDKAARAALVGPDADRSYNPYGTQIHET
                     STLARSAVKEEVLEAAANGTLQRTDYDDPALLARQARAHAASAIFAQRLKAKFARPQS
                     "
     gene            3372..3533
                     /locus_tag="VNU48_08225"
     CDS             3372..3533
                     /locus_tag="VNU48_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11299.1"
                     /translation="MFTFFETRIRPTAPPPGAPPAGLLAFYWHFIRQTRGLYLAMFAT
                     GLGVAISTR"
     gene            3539..5344
                     /locus_tag="VNU48_08230"
     CDS             3539..5344
                     /locus_tag="VNU48_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI11300.1"
                     /translation="MFIGKLVTLMQAADRVAAFSAATPMLLGMAAFLLIGRPAALLAD
                     SLVRNNAVVPSVTGLIRWQSHWHVVRQSWPFFQNDFAGRIANRVMQTGNAVRESVVSS
                     IRALWYIAIYGVSSLLLMGAADWRLAAPTVLWFAGYVFFLRYFVPRMRDRAKVSSELR
                     SMVMGRVVDSYTNILTVKLFARARDEDQYVREVIDAHTGAIAQHMRVTTRFMGTLAVM
                     NALLLVSTAAIGMLLWAQGQVSAGLVATALPLAWQIANVAGWVSWEVTGIFENVGVVQ
                     EGMQTIAVPHAMTDRPGARALVVRPAAEAARGPGRGEIRFEHLTFTYGRSDGKRVLDE
                     LNLVIKPGERIGLIGRSGAGKSTLVNLLLRFHEVEAGAIRIDGQDIGQVTQESLRAAI
                     GMVTQDTSLLHRSIAQNIGYGQPDATHAQIVAAARKAQAHDFILELSDWKGRTGYDAH
                     VGERGVKLSGGQRQRIAIARVVLKDAPILVLDEATSALDSEVELAIQEQLLGLMEGKT
                     VIAIAHRLSTIARMDRLVVLEQGRIVEQGTHDALLRLDGHYAKLWRHQSGGFLRLNSD
                     PVPVDVESEVQAALASAASDEPASAARHEPANAKA"
     gene            5351..>6631
                     /locus_tag="VNU48_08235"
     CDS             5351..>6631
                     /locus_tag="VNU48_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI11301.1"
                     /translation="MLRITELRLPLNHGADALRLGVVARLGLADADLESFTVFKRSYD
                     ARKKSAIVLIYTVDCALRDEAAVLARLAGQPHIRPTPDTGYRFVGHAPADWPVDRRPL
                     VIGFGPCGIFAALVLAQMGLRPIVLERGKAVRERTQDTWGLWRKGVLDPESNVQFGEG
                     GAGTFSDGKLWSQISDPRHLTRKVLGEFVKAGAPEEILFVAKPHIGTFRPVGVVEKMR
                     AEIEALGGEVRFGQRVTDVLIEGGQVRGVTLADGRQIAADHVVLALGHSARDTFAMLH
                     ERGVFMEPKPFSIGFRIEHPQSLIDSARFGPNAGNPILGAADYRIVHHARNGRSVYSF
                     CMCPGGTVVAATSEVGRVVTNGMSQYSRNERNANAAIVVGITPEDYAEFDPSGSPLAG
                     IALQRHWEEQAFRLGGESYDAPGQLVGDFLRKRAS"
BASE COUNT         1013 a         2342 c         2309 g          967 t
ORIGIN      
        1 agcgctgcac cttcagctgc agctccgggc cgctgtagac attgccatcg cggctgatgc
       61 gcacgtggcc gacggcggtc gcgaggtcct cggcctgctc gtagcgcagc cggtcggcgc
      121 tgatcttcat gccgccgcgg cggaactcgg cgttgccttc ggcgacggcg tcgaggtccg
      181 ggctggcggt cacggtctgc gcgtccagcc ggatcggcag gcggtgcgcc gcgtcgccgc
      241 gcggcgccgg atgcagcgtc ggcgacgcac gcagctcgat cggctcgtcc ggtccgctgg
      301 tctgcgcgca caccgagccc gccagcatcg cagccagcga tgcagccagg ggcgtgcgca
      361 gcgggcgcgc cgcgcggggt cgaaagagaa gcaccagacg tgaggaccga gagctgtggg
      421 cgacgggagc cggccggtgc ggtgccccgt gcgacgcaac cgtcgtcacc gggcctgccc
      481 ggggcagtgt ggtttgtaga atcgattatc cacgaggccc ccgacaagga ctgcttgagc
      541 accccgactc cccccgcgtc tgccgacgcc atcgcctggc ccgatccggc gcgccggcgc
      601 gcgttcgagg gctggctcga atcgatcgcg ccggcccacg cgctgcagcc gacgagcctg
      661 cgcgccgcgt ccagcgacgc gagcttccgg cgctacctgc gtgtcgacgg tgccggaagc
      721 agcttcatcg tgatggacgc gccgccgccg ctcgaggacg tgcgcccgtt cattcaggtg
      781 gccggcctgc ttcacgcggc cgggctgagc gcgccgcagg tgctcgagca ggatgtgccg
      841 catggcttcc tgctgctcac cgatctcggc tcgcacctgt acctcgcggc gctgcacgat
      901 gccgtcgcgc acggcgacga ggcgcggcgc aacgcgctga tgcgcgacgc gacgcgcgcg
      961 ctgatccgcc tgcagacgca ggtcgatgcg cgcccgctgc cgccttacga cgacgcactg
     1021 ctgcgacgcg agctcgcgct gttccccgac tggtgcctga cgcgcgagtg cggcgcaagc
     1081 tggtccgccg acgaacagca gcaatggcag gacgcctgcg acctgctcgt gcgcagcgcg
     1141 ctggcacagc ccacggtcgc ggtgcaccgc gactacatgc cgcgcaacct gatgctgctg
     1201 ggcggcgacg ccaaccccgg catcctcgac ttccaggacg ccgtgcgcgg cccgatcagc
     1261 tacgacctcg cctgcctgct gcgcgacgcc ttcatctcgt gggacgaaga gctcgagatc
     1321 gactgggccg tgcgctactg ggaggcggcg cgccgcgccg cgctgccgct gcccgacgac
     1381 ttcggcgagt tctggcgcca ggtcgagtgg atgggcttgc agcgccacct gaaggtgctg
     1441 ggcatcttct gccgtctgaa gcaccgcgat gccaagccga actacagcaa ggacctgccg
     1501 cgcttcttca actacgcgca caaggtggcg gcgcgctaca acgcgctgcg gccgctgtcg
     1561 cgcctgctcg aaccgctgat gggcgcgcag cgcgtcgagg gcttctactg agcgacgcgg
     1621 ccacagccta gggcgcgagc gcgagcgagg cctgcaccgg cgcgcgcttg ccgatcggct
     1681 gcgtctcgca gccggccgcg agcagccacg ggtcacggtc ctcgccgccg aaggtcttga
     1741 ccaggaagtc gacgaacacc cgggtgcgcg ctggcacgtg cttgcgcgtg ggcatgccgg
     1801 catacaacgt attcgtgaac agatgccatt gcggcagcac gcgttcgagc gcgttctcga
     1861 gcagcgcgtc ctcggccacg aacgacggca gcccggtgat gcccaggccg gccagcgccg
     1921 cggcgtacat cgtgtcggtg tggatcgtgc tcaggcccgc gcgcttgggg ctgagcgtga
     1981 acgattcgcc cgccgcgtcg gcgtcgcctg gcgcgccgac gtggaaggtc agttcgcgca
     2041 ggaacggcgg caccatgctc tcgtggccat ccatgtcctt cgggtgctgc ggtcggccgc
     2101 gccgatcgag gtactcgggt gcggcgcaca ggatcacctc ggaacgcgcc agtcgccgtg
     2161 cgacaaaatt gccgtccagt gggcgccggc cctcggtcag gatgctgaca tcgaagttct
     2221 cgtcgaccgt ctcgaccggc cccggcaccg agagctcgag cgacacgcgc gggtagagcg
     2281 cccggaacct cggcagatgc ttggcgatct ggtgcaccgc gaatgccggc gagcacagca
     2341 cgcgcagatg cccgcgcggc tcggaggtcg ccgcggtcgc gagcgcctcg gcttcctcga
     2401 cctcggtcag gatgtggcgc acccgctcca ggtacagctc gccggtgtcg gtcagcgaca
     2461 gccggcgcgt ggtgcggttg atgagccgcg agccgaggtg ctcttcgaga tcggcgacca
     2521 gccgggtcac gaccgccggc gagaggttca gctcgcgggc ggcgcgggcg aagctgccct
     2581 catcgacgac gcgcgagaac acgcgcatgg aatgcagtcg gtccattttt caacccccgc
     2641 ctttgtttct ctttgagcaa tgcgcaattg aaccacacgc tgtttattga cgcttcggca
     2701 atgcctacag ttcagtccat cgatgaggaa gacctcaccg aggctggaca agacggcaac
     2761 aaagaacaac cgccgcgctg cccggaacgt caatcacatt gaatcggaag tcaccatcat
     2821 gaacagcaag tttctctacg ccgccaccgt cgccatctcc ctgatcagca ccttcgcgat
     2881 ggccgacgaa gcgccggcca cgcgcgccca ggtcaacgcc gaactgcgtc aggcgatcgt
     2941 cgacggcacg ctgcagcgca gcgactacag cgcggactcg tatcgcctgc tcgcgcgcac
     3001 ctccaccaag acccgcgcgc aggtcggcgt cgaactggcg caggacaagg ccgcgcgcgc
     3061 cgcgctggtc ggcccggatg cggaccgcag ctacaacccc tacggcacgc agatccacga
     3121 gacctcgacg ctggcgcgct cggcagtgaa ggaagaagtg ctcgaggcgg ccgcgaatgg
     3181 cacgctgcag cgcaccgact acgacgaccc ggccctgctc gcgcgtcagg cgcgcgccca
     3241 cgcggcatcg gccatcttcg cccagcgcct gaaggcgaag ttcgcgcggc cgcaaagctg
     3301 agcgccaggc cgcgccacga ccaggccgcc ttcgggcggc ctttgtcatg gccggcgaga
     3361 atacgcggtc gatgttcacc ttcttcgaaa cccgcatccg cccgaccgcg ccgccgcccg
     3421 gcgccccgcc cgccggcctg ctcgcgttct actggcactt catccgccag acccgcggcc
     3481 tgtacctcgc gatgttcgcc accggcctgg gcgtggcgat atcgacacgc tgatcccggt
     3541 gttcatcggc aagctcgtga ccctgatgca ggccgccgat cgtgtggcgg cgttcagcgc
     3601 ggccacgccg atgctgttgg gcatggccgc gttcctgctg atcggccgcc cggcggcgct
     3661 gctggccgac agcctggtgc gcaacaacgc ggtcgtgccc agcgtgaccg ggctgatccg
     3721 ctggcagagc cactggcacg tggtgcggca gagctggccg ttcttccaga acgacttcgc
     3781 cgggcgcatc gcgaaccgcg tgatgcagac cggcaacgcg gtgcgcgagt cggtggtcag
     3841 cagcatccgc gcactctggt acatcgcgat ctacggcgtc tcgtcgctgc tgctgatggg
     3901 cgcggccgac tggcgcctgg cggcgcccac cgtgctgtgg ttcgccggct atgtgttctt
     3961 cctgcgctac ttcgtgccgc gcatgcgcga ccgcgcgaag gtcagctccg agctgcgctc
     4021 gatggtgatg ggccgggtcg tcgacagcta caccaacatc ctgacggtca agctgttcgc
     4081 ccgcgcgcgc gacgaggacc agtacgtgcg cgaggtgatc gacgcgcaca ccggcgcgat
     4141 cgcgcagcac atgcgcgtga cgacgcgctt catgggcacg ctggcggtga tgaacgcgct
     4201 gctgctggtc agcaccgcgg cgatcggcat gctgctgtgg gcgcagggcc aggtcagcgc
     4261 cgggctggtc gcgaccgcgc tgccgctggc ctggcagatc gccaacgtcg ccggctgggt
     4321 gagctgggaa gtgaccggca tcttcgagaa cgtcggcgtc gtgcaggaag gcatgcagac
     4381 gatcgcggtg ccgcacgcga tgaccgatcg ccccggcgcg cgcgcgctcg tcgtgcggcc
     4441 cgcagccgag gctgcgcgcg ggccgggtcg cggcgagatc cgcttcgagc acctgacctt
     4501 cacctacgga cgcagcgacg gcaagcgtgt gctcgacgag ttgaatctgg tcatcaagcc
     4561 gggcgagcgc atcgggctga tcggccgctc gggcgccggc aagtcgacct tggtgaacct
     4621 gctgctgcgc ttccacgagg tcgaggccgg ggccatccgc atcgacggcc aggacatcgg
     4681 ccaggtcacg caggaaagcc tgcgcgccgc gatcggcatg gtcacgcagg acacctcgct
     4741 gctgcaccgc tcgatcgcgc agaacatcgg ctacggccag cccgacgcca cgcacgccca
     4801 gatcgtcgcg gccgcgcgca aggcgcaggc gcacgacttc atcctcgaac tgtccgactg
     4861 gaagggccgc accggctacg acgcgcacgt cggcgagcgc ggcgtcaagc tgagcggcgg
     4921 ccagcgccag cgcatcgcga tcgcgcgcgt ggtgctgaag gacgcgccga tcctggtgct
     4981 cgacgaagcg acctcggcgc tcgacagcga ggtcgagctc gcgatccagg agcagctgct
     5041 cggcctgatg gaaggcaaga ccgtgatcgc gatcgcgcac cggctgtcga cgatcgcgcg
     5101 catggaccgg ctggtcgtgc tcgagcaggg gcgcatcgtc gagcagggca cgcacgacgc
     5161 gctgctgcgc ctggacggcc actacgcgaa gctgtggcgg caccagtcgg gcggcttcct
     5221 gcgcctgaac agcgacccgg tgccggtcga tgtcgagtcc gaagtgcagg ccgcactcgc
     5281 gagtgcggcg agcgacgagc ccgcgagcgc ggcgcgccac gagccggcga acgcgaaggc
     5341 ctgagcggag atgctgcgca tcaccgaact gcggctgccg ctgaaccatg gagcggatgc
     5401 gctgcgcttg ggcgtcgtcg cgcgcctcgg gctggccgat gccgatctcg agagtttcac
     5461 cgtgttcaag cgcagctacg acgcgcgcaa gaaaagcgcg atcgtgctca tctacacggt
     5521 cgactgcgcg ctgcgcgacg aggccgccgt gctcgcgcgc ctcgccggcc agccgcacat
     5581 ccggccgaca cccgacaccg gctatcgctt cgtcggccac gcgccggccg actggcccgt
     5641 cgaccggcgt ccactggtga tcggcttcgg cccgtgcggc atcttcgcgg cgctggtcct
     5701 ggcgcagatg ggcctgcgcc cgatcgtgct ggagcgcggc aaggcggtac gcgagcgcac
     5761 ccaggacacc tggggcctgt ggcgcaaggg cgtgctggac cccgagtcga acgtgcagtt
     5821 cggcgagggc ggcgccggca ccttctccga cggcaagctg tggagccaga tcagcgaccc
     5881 gcgccacctg acgcgcaagg tgctgggcga gttcgtgaag gccggcgcgc ccgaagagat
     5941 cctcttcgtc gcgaagccgc acatcggcac cttccggccc gtcggcgtgg tcgagaagat
     6001 gcgcgccgag atcgaggcgc tgggcgggga ggtccgcttc gggcagcgcg tcaccgacgt
     6061 gctgatcgaa ggtggccagg tgcgtggcgt gaccctcgcc gatggccggc agatcgccgc
     6121 cgaccatgtc gtgctcgcgc tcggccacag cgcgcgcgac accttcgcga tgctgcacga
     6181 acgcggcgtg ttcatggagc ccaagccgtt ctcgatcggg tttcggatcg agcacccgca
     6241 gtcgctgatc gacagtgcgc gcttcgggcc gaacgccggc aacccgatcc tcggcgcggc
     6301 cgactaccgg atcgtccacc acgcgcgcaa tggccgctcg gtctacagct tctgcatgtg
     6361 cccgggcggc accgtcgtcg ccgcgacctc ggaggtcggc cgcgtcgtca cgaacggcat
     6421 gagccagtac tcgcgcaacg aacgcaacgc caacgcggcc atcgtggtcg gtatcacgcc
     6481 ggaggactac gccgagttcg accccagcgg cagtccgctg gccggcatcg ccttgcagcg
     6541 gcactgggag gaacaggcct tccgcctcgg tggtgaaagc tacgatgcgc cgggacagct
     6601 ggttggcgat tttctgcgca agcgcgcatc c
//
LOCUS       DATMJB010000380         2154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_484884, whole genome shotgun sequence.
ACCESSION   DATMJB010000380 DATMJB010000000
VERSION     DATMJB010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484884"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1734
                     /gene="cysC"
                     /locus_tag="VNU48_08240"
     CDS             <1..1734
                     /gene="cysC"
                     /locus_tag="VNU48_08240"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HWI11302.1"
                     /translation="ALEADSKKVGTRGDELDYALLLDGLAAEREQGITIDVAYRFFST
                     DKRKFIVADTPGHEQYTRNMVTGASTADLAIILIDARKGMLVQTRRHSYLVSLIGIRH
                     VIVAVNKMDLVDYSQEVFSKIESEYREFAAKIGLEGMTFMPISGVKGDNIIEQGPNTP
                     WYHGPTLMHHLETVEIDELRLQKQPFRLPVQWVNRPNLDFRGFAGTIAGGSVRPGQRI
                     RVQPSGRESTVQRIVTADGDLDLAVANQSITLTLADEVDISRGDVISTVEAPAEVADQ
                     FEATVVWMSDQPMLRGRNYLMKIGAKTVTATVAPLKHKINVNTLEHVAANKLELNEIG
                     VVDLELDRAIAFDAYETNRDTGGFILIDRLTNSTVGAGMLRFALRRAQNVHWQAIDVN
                     KQAHASIKGQKPCVLWFTGLSGAGKSTIANMVEKKLFSEGRHTYLLDGDNVRHGLNKD
                     LGFTDEGRVENIRRVSEVAKLMIDAGLIVITAFISPFRSERQSARALLEAGEFVEVFI
                     DTPITVAEERDPKGLYKKARSGQLANFTGIGSPYEAPQNAEIRIDTTKMTPDAAAEFI
                     VAALRERGMLD"
     gene            1836..>2154
                     /locus_tag="VNU48_08245"
     CDS             1836..>2154
                     /locus_tag="VNU48_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI11303.1"
                     /translation="MSEIVRFDYSGVRVIVTGATSGIGLATARAFAAAGADVLVTGTR
                     HALEAYGESAPVAGASYRRLELDQAAAIEAFAAAVEQVDVLVNNAGSIMPGADFASAV
                     QVNL"
BASE COUNT          418 a          688 c          704 g          344 t
ORIGIN      
        1 gccctcgaag ccgattcgaa gaaggtgggc acgcgcggcg acgaactcga ctacgcgctg
       61 ctgctcgacg ggctggcggc cgagcgcgag caaggcatca cgatcgacgt cgcctaccgc
      121 ttcttctcga ccgacaagcg caagttcatc gttgccgaca cgccggggca cgaacagtac
      181 acgcgcaaca tggtcaccgg cgcttcgacg gccgatctgg cgatcatcct catcgatgcg
      241 cgcaaaggca tgctggtgca gacccgccgc cacagctacc tggtgtcgct gatcggtatc
      301 cggcatgtca tcgtcgccgt caacaagatg gacctggtcg actattcgca ggaggtcttc
      361 tcgaagatcg aaagcgagta ccgcgagttc gccgccaaga tcggcctcga aggcatgacc
      421 ttcatgccga tttccggcgt gaagggcgac aacatcatcg agcaggggcc gaacacgccg
      481 tggtaccacg ggccgaccct gatgcaccat ctggagacgg tcgagatcga cgaactgcgc
      541 ctgcagaagc agccgtttcg cctgcccgtg cagtgggtca accgcccgaa tctggatttc
      601 cgcggcttcg ccggcacgat cgcaggcggg tcggtccgac ccggtcagcg cattcgcgtg
      661 cagccctcgg gccgggagag cacggtccag cgcatcgtca ccgcggacgg cgatctggac
      721 ctggccgttg ccaaccagtc gatcaccctg acattggccg acgaagtcga catctccagg
      781 ggcgacgtga tctccaccgt cgaagcaccg gcggaggtgg ccgaccagtt cgaggcgacc
      841 gtggtctgga tgagcgacca gccgatgctg cgcggacgaa attacctgat gaagatcggc
      901 gcgaagacgg tcacggcgac agtcgccccg ctcaagcaca agatcaacgt caacacgctc
      961 gaacacgtgg ccgcgaacaa gctcgagctc aacgagatcg gcgtcgtcga tctcgagctg
     1021 gaccgcgcga ttgccttcga cgcgtacgag acgaaccgcg acaccggtgg cttcatcctg
     1081 atcgaccgtc tgaccaacag cacggtcggc gccggcatgc tgcgctttgc cttgcgtcgc
     1141 gcgcagaacg tgcactggca ggccatcgac gtgaacaagc aggcccacgc ctccatcaag
     1201 ggccaaaagc cgtgcgtctt gtggttcacc gggctgtcgg gtgccggcaa atccacgatc
     1261 gcgaacatgg tcgagaagaa gctgttctcc gaagggcggc acacctacct gctcgatggc
     1321 gacaacgtgc gccacgggct gaacaaggac ctcggtttca ccgacgaagg acgggtcgag
     1381 aacatccgcc gcgtcagcga agtggccaag ctgatgatcg atgccggcct gatcgtgatc
     1441 accgcgttca tttcgccgtt ccgcagtgaa cgccagtcgg cacgggcctt gttggaagcc
     1501 ggtgagtttg tcgaggtctt catcgacaca ccgatcacgg tggcagaaga gcgcgatccc
     1561 aagggcctgt acaagaaggc gcgcagcggc cagcttgcga acttcaccgg catcggctcg
     1621 ccgtacgagg caccccagaa tgcagagatt cggatcgaca cgaccaagat gacaccggat
     1681 gccgctgccg aattcatcgt cgcggcgctg cgtgaaagag gcatgctcga ctgatttgac
     1741 gcggctgggg cggaccgcgc tggcgggccc gcggtgtcgc ggggtgccgc agcagaatgg
     1801 gtgtgcccgg gatcgaagaa gggcggaggt tcagcatgtc ggagattgtt cgctttgact
     1861 attccggggt ccgcgtgatc gtcaccggag ccaccagcgg catcggcctg gcgaccgcgc
     1921 gcgcgtttgc cgcggccgga gctgacgtgc tggtcaccgg gacgcgccac gcgctggagg
     1981 cctacggcga gtctgcgccg gttgccggcg cctcctatcg ccgactcgaa ctcgaccaag
     2041 cggcggccat cgaggccttt gccgcggcgg tcgagcaggt tgacgtgctg gtgaacaacg
     2101 ccggcagcat catgcccggg gcggacttcg cgagcgcggt gcaggtgaac ctca
//
LOCUS       DATMJB010000381         5248 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_403043, whole genome shotgun sequence.
ACCESSION   DATMJB010000381 DATMJB010000000
VERSION     DATMJB010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403043"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VNU48_08250"
     CDS             <1..576
                     /locus_tag="VNU48_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWI11304.1"
                     /translation="SALLEAMEEQQVTVEGETRALPRPFFVIATQNPSDQLGTYPLPE
                     SQLDRFLMCLTLGYPDQASERALLAGQDRREAIHVLRAVMTPAQLVAAQLAVQAVHVS
                     DALLDYLQALIAATRSGRWFVEGLSPRAGIGVLRAARARALLAQRDYVAPDDVQYVLP
                     QTIAHRLVPVAGAGRGRVGQVRAMVEAVPIP"
     gene            581..1585
                     /locus_tag="VNU48_08255"
     CDS             581..1585
                     /locus_tag="VNU48_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWI11305.1"
                     /translation="MAGAAASVLAGLNPAGFARARFRAWINARQPRTDTLLLTQRNVY
                     ILPTRAGWMFGATLLVLLVGSVNYQLNLGYVLTFLLAGSGVVSMHLTHATLRGLTLHL
                     KPVAPVFAGDAAVLDLVLTSPGAARFGIGLKVAGAPEATLTWINVPALGQAHAQVSFV
                     PEMRGWHAAPTLGAETRFPLGLFRVWTVWRPAAQLLVYPRPEFPAGPLPPARPVPGGP
                     TESRSADGGEVEGVRAYRRGDPLKLIAWKKAAKALDTGGDLVSRDTSASAHQELWLAW
                     QACGNLAPEERLSRLTAWTLAAHRAGSDYGLRLPGVEIAPADGDAQRRQCLEALALWQ
                     "
     gene            1695..3716
                     /locus_tag="VNU48_08260"
     CDS             1695..3716
                     /locus_tag="VNU48_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HWI11306.1"
                     /translation="MTQWPGWRRLPRDARDTLFLLAVIGWTVLPHVSHLPAWCSLLTL
                     TVLLWRARLAIANAALPNRWVLVAVLLLAAALTFWSHRTLLGKEAGVTLAVVLMALKT
                     LELRARRDAFVVFFLGFFLILTHFLYSQSLPVAAAMLVSVWGLLTALVLAHMPVGQPS
                     LRQAARLSARTALFGAPIMALLFVLFPRIGPLWGVPQDGQSTTGLSNSMRMGSVAEVA
                     QDDSIALRIRFDGTPPPPGALYFRGPVLSHFDGVEWQAQPPSFGAFAAPRRNLRTQGT
                     PLRYEMTIEPSRLGALPLLEAAADAPKLEGMRVFGLPDLQWRADRPLYERVRLRAEAF
                     IDFRHGPVAAEPSLRDQLELPPGYNPRTLAWAAALRAEPRYANADARVLAQALLEHIR
                     SDGFSYTLAPGEYGRDAVDEFWLDRREGFCEHFAAAFVVLMRALDVPARVVTGYQGVD
                     PFASDGYYVVRQSSAHAWAEYWVAGIGWVRADPTAAVAPERINRGARLLPARGFVAGA
                     IANVSPQLMDRLRGSWEALNNRWNQWVLNYSRGQQLDLLKDLGVSSPSWEDLALLLIG
                     ALSSLALLGAAWAWWDRHRVDPWTRQMDRLRAALQRTGLSAAPHEPPRALAQRVRERF
                     GARGEPLAELLDALERQRYSRATIARPDAALTRQFVTRARVLRPESAHR"
     gene            3724..4848
                     /gene="mltB"
                     /locus_tag="VNU48_08265"
     CDS             3724..4848
                     /gene="mltB"
                     /locus_tag="VNU48_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HWI11307.1"
                     /translation="MPRSFPSLRSTTLALLATALLVTTGLALARPKKPAPDAALADDA
                     PDVVTYGRRDDVMRFGAELAERRGLDAEWVQDALQRARFVPLVTKFIMPPPAGSAKNW
                     AAYRARFIDPVRVRAGAAFWRANEKWLELAEELYGVPPEIVVGIVGVETIYGQQMGNF
                     RVVDALATLAFDFPTGRKDRSAFFRDELESFFVMCQSEGIDPLQQKGSFAGAMGMPQF
                     MPSSFNKYAVDLDGDGHVDLRGNPADVIGSVAHYLAESGWRRGLPTRFEVAVPVATTD
                     RAILLAPDILPSFTLAEFAERGAQLNAAAFAADVRLADAGGVGKLALVELQNGDAAPS
                     YVAGTANFYAVTRYNWSSYYALAVIELGEAVAAELERGAR"
     gene            complement(4859..>5248)
                     /locus_tag="VNU48_08270"
     CDS             complement(4859..>5248)
                     /locus_tag="VNU48_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11308.1"
                     /translation="SHRMASTGDGEMAHFHFNLRSLIAAPAARDAAWRRSLDELPADD
                     AIEIVNGPGWFDSSWDLVHGLEVREGLPGDAQLHEWLEVWLRDERVAPVASRPARHPG
                     VLQRAAAPAPVRDERFDAFDIEGLELA"
BASE COUNT          659 a         1921 c         1902 g          766 t
ORIGIN      
        1 agcgcgctgc tcgaagcgat ggaggagcag caggtcaccg tcgaaggcga gacgcgcgcg
       61 ctgcccaggc ccttcttcgt catcgcgacg cagaacccga gcgatcagct cggcacctac
      121 ccgctgcccg agtcgcagct cgatcgcttc ctgatgtgcc tcacgctcgg ctaccccgac
      181 caggcgagcg aacgcgcgct gcttgccggc caggaccggc gcgaggcgat ccacgtgctg
      241 cgtgcggtga tgacgcccgc tcagctcgtc gccgcgcagc tggcggtgca ggcggtgcac
      301 gtctcggatg cactgctcga ctacctgcag gcgctgatcg cggcgacgcg ctcggggcgc
      361 tggttcgtcg agggcctgtc gccgcgcgcc ggcatcggcg tgctgcgtgc cgcgcgggcg
      421 cgggcgctgc tggcccaacg cgactatgtc gcgcccgacg acgtgcagta cgtgctgccg
      481 cagaccattg cgcaccggct cgtgccggtg gccggcgcgg gccgcgggcg cgtcgggcag
      541 gtgcgggcga tggtcgaggc ggtgccgatc ccgtagcgag atggccggag ccgctgcgtc
      601 cgtgctggcc ggcctgaacc cggccggctt cgcgcgcgcc cgtttccggg cctggatcaa
      661 cgcgcgccag ccgcgcaccg acacgctgct gctgacgcag cgcaacgtgt acatcctgcc
      721 gactcgtgcg ggctggatgt tcggtgcgac cttgctggtg ctgctcgtcg gctcggtcaa
      781 ctaccagttg aacctgggct atgtgctgac cttcctcctt gccggcagcg gcgtcgtgtc
      841 gatgcacctg acgcacgcca cgctgcgcgg gctgacgctg cacctgaagc cggtcgcgcc
      901 ggtcttcgcg ggcgacgcgg cggtgctcga cctggtgctg accagccccg gcgcggcgcg
      961 cttcggcatc gggctgaagg tggcgggtgc gccggaggcg acgctgacct ggatcaacgt
     1021 gccggcgctc ggccaggccc atgcgcaggt cagcttcgtg ccggaaatgc gcggctggca
     1081 tgcggcgccg acgctgggcg cggagacgcg cttcccgctc gggctgttcc gggtctggac
     1141 ggtctggcgg ccggccgcgc agttgctggt ctacccgcga cccgagttcc cggccggccc
     1201 gttgccgccg gcgcggccgg tgcctggcgg gccgaccgag tcgcgcagcg cggacggcgg
     1261 cgaggtcgag ggcgtgcgcg cctaccgccg cggcgacccg ctgaagctga tcgcgtggaa
     1321 gaaggccgcg aaggcgctcg acaccggcgg cgacctggtc agccgcgaca ccagcgcgtc
     1381 ggcacaccag gagctgtggc tcgcctggca ggcctgcggc aacctcgcac ccgaggagcg
     1441 gctgtcgcgc ctgaccgcgt ggaccctggc cgcgcaccgc gccggcagcg actacgggtt
     1501 gcgactgccc ggcgtcgaga tcgcgccggc cgacggcgat gcgcagcgcc gccagtgtct
     1561 ggaggcgctc gcgctgtggc agtgacacgc tcacggtcga ctcgccgcga gcgggccgag
     1621 cgccgggccg ctcccaagcc ggcccgcgtc ccctcggggg accggccgat gtacccatcg
     1681 gccgaggggc accaatgacg cagtggccgg gctggcggcg cctgccgcgc gacgcgcgcg
     1741 acacgctgtt cctgctcgcg gtgatcggct ggacggtgct cccgcatgtc tcgcacctgc
     1801 cggcatggtg ctcgctgctg acgctcacgg tgctgctgtg gcgcgcgcga ctcgcgatcg
     1861 ccaacgcggc gctgccgaac cgctgggtgc tggtcgcggt gctgctgctc gcggcggcgc
     1921 tgaccttctg gagccaccgc accctgctcg gcaaggaggc cggcgtgacg ctggcggtgg
     1981 tgctgatggc gctgaagacg ctcgaactgc gcgcgcgccg cgatgccttc gtcgtgttct
     2041 tcctcggctt cttcctgatc ctgacgcact tcctgtattc gcagtcgctg ccggtggccg
     2101 ctgcgatgct ggtctcggtc tggggcctgc tgaccgcgct ggtgctcgcg cacatgccgg
     2161 tcggccaacc gagcctgcgc caggccgcgc ggctgtcggc acgcaccgcg ctgttcggcg
     2221 cgccgatcat ggcgctgctg ttcgtgctgt tcccgcgcat cgggccgctg tggggcgtgc
     2281 cgcaggacgg gcagagcacg accggcctgt cgaactcgat gcgcatgggc tcggtcgccg
     2341 aggtcgcgca ggacgacagc atcgcgctgc gcatccgctt cgacggcacg ccgccaccgc
     2401 ccggcgcgct gtactttcgc ggcccggtgc tgtcgcattt cgacggcgtc gagtggcagg
     2461 cgcagccgcc ctccttcggc gccttcgcag cgccgcgccg caacctgcgc acgcagggca
     2521 cgccgctgcg ctacgagatg acgatcgagc cgtcgcgcct gggcgcgctg ccgctgctcg
     2581 aggccgccgc cgacgcgccc aagctcgaag gcatgcgcgt gttcggcctg cccgacctgc
     2641 agtggcgcgc cgaccggccg ttgtacgagc gcgtgcggct gcgtgccgag gccttcatcg
     2701 acttccgcca tggcccggtc gccgccgagc cgagcctgcg ggaccagctc gagctgccgc
     2761 ccggctacaa cccgcgcacg ctcgcctggg cggcggcgct gagagccgag ccgcgctatg
     2821 cgaacgccga cgcgcgtgtg ctcgcgcagg cactgctcga gcacatccgc agcgacggct
     2881 tcagctacac gctggcgccc ggcgagtacg gacgcgatgc ggtcgacgag ttctggctcg
     2941 accgccgcga gggcttctgc gaacacttcg ccgccgcgtt cgtcgtgctg atgcgcgcgc
     3001 tcgatgtgcc cgcgcgcgtc gtcaccggct accagggcgt cgacccgttc gcgagcgacg
     3061 gctactacgt cgtgcgccag agttcggcgc acgcctgggc cgagtactgg gtcgccggca
     3121 tcggctgggt gcgcgccgac ccgaccgccg cggtcgcgcc cgaacgcatc aaccgcgggg
     3181 cgcggctgct gccggcgcgc ggcttcgtcg ccggggcaat cgccaacgtc agcccgcagc
     3241 tgatggatcg gctgcgcggc agctgggagg cgctgaacaa ccgctggaac cagtgggtgc
     3301 tgaactactc gcgcggccag cagctcgatc tgctgaagga cctgggcgtg tcctcgccga
     3361 gctgggaaga cctcgcactg ctgctgatcg gggcgctgag ttcgctcgcg ctgctcggcg
     3421 ccgcctgggc ctggtgggac cggcaccgcg tcgacccgtg gacgcgccag atggaccgcc
     3481 tgcgcgccgc gctgcagcgc accggcctca gtgccgcgcc gcacgagccg ccgcgtgcgc
     3541 tcgcgcagcg cgtgcgcgaa cgtttcggcg cgcgcggcga gccgctcgcc gagctgctcg
     3601 acgccctcga acgccagcgc tacagccgcg ccacgatcgc gcggcccgac gcggcgctga
     3661 cgcgccagtt cgtcacgcgc gcccgagtgc tgaggcctga atcggcgcac cgataatcgg
     3721 gcgatgcccc gaagcttccc atcactgcgc agcacgacgc tggcgctgct cgcgaccgcg
     3781 ctgctggtca ccaccggcct tgcgctggcg cgcccgaaga agccggcgcc cgacgccgcg
     3841 ctcgccgacg acgcgcccga tgtcgtcacc tacggccggc gcgacgacgt gatgcgcttc
     3901 ggcgccgagc tcgccgagcg ccgcggtctc gacgccgagt gggtgcagga cgccctgcag
     3961 cgcgcgcgct tcgtcccgct ggtcacgaag ttcatcatgc cgccgccggc cggcagcgcg
     4021 aagaactggg ccgcgtaccg cgcccgcttc atcgacccgg tgcgcgtgcg cgccggcgcc
     4081 gcgttctggc gcgccaacga gaagtggctc gagctcgccg aagagctgta cggcgtgccg
     4141 cccgagatcg tcgtcggcat cgtcggcgtc gagacgatct atggccagca gatgggcaac
     4201 tttcgcgtcg tcgatgcgct cgcgacgctg gcgttcgact tccccaccgg ccgcaaggac
     4261 cgcagcgcgt tcttccgcga cgagctcgag agtttcttcg tgatgtgcca gagcgaaggc
     4321 atcgacccgc tgcagcaaaa gggcagcttc gccggcgcga tgggcatgcc gcaattcatg
     4381 ccgtcgagct tcaacaagta cgcggtcgac ctcgatggcg atggtcacgt cgacctgcgc
     4441 ggcaacccgg ccgacgtgat cggcagcgtc gcgcactacc tggccgagtc cggctggcgg
     4501 cgcggcctgc cgacgcgctt cgaggtcgcg gtgccggtgg cgacgaccga ccgcgcgatc
     4561 ctgctcgcgc ccgacatcct gcccagcttc acgctcgccg agttcgccga gcgtggcgcg
     4621 caattgaatg ccgcggcctt cgcggccgac gtgcgcctgg ccgacgcggg cggcgtcggc
     4681 aagctcgcac tggtcgagct gcagaacggc gacgcggcac cgagctacgt cgcgggcacc
     4741 gccaacttct atgcggtgac gcgctacaac tggtcgagct actacgcgct ggcggtgatc
     4801 gagctcggcg aagcggtcgc ggccgaactc gagcgcggcg cgcgctgacg gcgcagcgtc
     4861 acgcgagttc gaggccctcg atgtcgaagg catcgaagcg ctcgtcgcgg accggtgcgg
     4921 gcgccgccgc gcgctgcagc acgccgggat ggcgcgccgg tcgggatgcg accggcgcga
     4981 cgcgctcgtc gcgcagccac acctccagcc actcgtgcag ctgcgcgtcg ccgggcaggc
     5041 cttcgcgcac ctcgagaccg tgcacgaggt cccagctcga gtcgaaccag cccgggccat
     5101 tgacgatctc gatcgcgtcg tcggcgggca actcgtccag gctgcggcgc caggccgcgt
     5161 cgcgtgccgc cggcgcggcg atgaggctgc gcagattgaa gtgaaagtgg gccatttctc
     5221 cgtctcccgt cgatgccatt cgatggga
//
LOCUS       DATMJB010000382         4885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_854644, whole genome shotgun sequence.
ACCESSION   DATMJB010000382 DATMJB010000000
VERSION     DATMJB010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4885
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854644"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1815
                     /locus_tag="VNU48_08275"
     CDS             <1..1815
                     /locus_tag="VNU48_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWI11309.1"
                     /translation="MPKAFEVHAKVGYSSQPFSLYGTDSSYRAWETSASVGNRSGAFA
                     WLLNFDHGDSHGQPLTFATRLLSSGTPGSAGTPVSGAVPGANNANQPWYVLGTGTEYR
                     SRQDQFKAKLAWDITPAIRASAVLGVWRNSADGTPVSYLRNAAGQPVYGGVVNIGGQS
                     FAALTGADFAATSERLTHVMQSMAVKSHTQGPWDWELDASRYDYRRDDKRQNGIGNVL
                     PDALYGGAGTLADGSGTGWVNLVAKGTWRPQGMTGAHIVDFGAQHDVYKLRYLTSNIA
                     GNWLADPAGTLASSVGGKTQLHSVYAQDAWAFAPRWKTVLGVRAEDWRADDGATAFSA
                     TSTLRYPSRHERHLSPKAALSYQAAPDWVLKAAAGRALRMPTVSELYGATSTTNSQYI
                     NDPSLKPEKSWTTELSAEHDLGHALLRLTCFSENTHDSLYAQTTFDPVADKNIGRVQN
                     VGRMRTNGLELAFNGSDVLRRGLDFNASVTYADSVIKANDGFVVTPGDTLGKRQPNIP
                     RWRATALVSYRVDERWSAAFGARYSGTQYRTLNNADVSGSTYQGVSRYFTTDLRLRWQ
                     IDRQWSAAFGIDNLNNDRYWNFHPYPQRSYSAELRFDL"
     gene            1859..2350
                     /locus_tag="VNU48_08280"
     CDS             1859..2350
                     /locus_tag="VNU48_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HWI11310.1"
                     /translation="MPLSKLLKLAACSAVFAVASAHAHGGHAGDVEITHPFATPMPAG
                     ASNGAAYVATLENTGKQADRLIRVSTPIAQRAEIHTMAVDAGGVMRMREVGEIALPPG
                     VLVKMRPGEGYHFMLMGLKQPLKEGDSFPLMLEFEHGGKAEVKVVVQVPKARPGEMAE
                     HKH"
     gene            complement(2435..3007)
                     /gene="ruvA"
                     /locus_tag="VNU48_08285"
     CDS             complement(2435..3007)
                     /gene="ruvA"
                     /locus_tag="VNU48_08285"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWI11311.1"
                     /translation="MIGRLTGTLAEKSPPHLLIDVNGVGYEVDVPMSSFYNLPGLGER
                     VTLLTHFVVREDAQVLFGFLTHDERATFRLLVKISGVGPRTALSILSGLSVNELAQAV
                     GLQESARLVKVPGIGKKTAERLLLELKGKLGDAIATTTSVASHAQGDILQALLALGYS
                     DREAAAALKALPPDVGVSDGIKLALRALAK"
     gene            complement(3036..3692)
                     /locus_tag="VNU48_08290"
     CDS             complement(3036..3692)
                     /locus_tag="VNU48_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI11312.1"
                     /translation="MDNILLLEDLPEIRSWLKVLIKQVFPNDQIHEAARVHDALALVT
                     AVKFKLALVDLGLPDGSGVDVVAALREAQSECQSVVVTIHDDDDHLFPALQAGAFGYL
                     LKEQARELIAEQLNRMSQGEPPLSPSIARRVISHFAAQTRPQERPQNMPHVALTDREN
                     EVLLRVAKGFTLPEIGVQLNLSRHTIADYVKQIYRKLNVSSRAEAALEAQRLGLFRRA
                     "
     gene            complement(3799..>4885)
                     /locus_tag="VNU48_08295"
     CDS             complement(3799..>4885)
                     /locus_tag="VNU48_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI11313.1"
                     /translation="GFAPLVALAVQFLLSFAGLRSRWIENALVAQWVLLPATLLLAGP
                     GRLFLMASIWYAALAAEVVGAAVLYLVVTWRGQRQDFWSMAALLGAVVALLGAEFARQ
                     HDLLPPLGVPLLQIALPLVFIALAMRLLQVFARALRAAEAGRENLESRVHEITAEIER
                     NFAQLAELRVEQVTEKERKRIAADLHDDLGAKLLTIVHTSESERISTLAREALEEMRL
                     SVRGLTGKPMRLADALADWRAETVSRLGQANIEADWRGPTEEIEQLLPARGFVQTTRI
                     LREAVSNIIKHSGASHCKVRCAISERQFGLTVQDNGKGIPMELDGKLDRGHGMASMKH
                     RAKQMQGQCLVESGAGYGTVIRLTLPL"
BASE COUNT          784 a         1740 c         1665 g          696 t
ORIGIN      
        1 atgccgaagg ccttcgaggt gcatgcgaag gtcggctaca gcagccagcc cttcagcctg
       61 tacggcaccg acagcagcta ccgcgcctgg gagacgagcg cctcggtggg caaccgcagc
      121 ggcgcgttcg cgtggctgct gaacttcgac catggcgaca gccacggcca gccgctgacc
      181 ttcgcgactc ggctgctgag cagcggcacg ccgggcagcg ccggcacgcc ggtgagcggc
      241 gccgtgcccg gtgcgaacaa cgcgaaccag ccctggtatg tgctcggcac aggcaccgaa
      301 taccgcagcc ggcaggacca gttcaaggcc aagctcgcct gggacatcac gcctgcgatc
      361 cgcgccagcg ccgtgctcgg cgtctggcgc aacagcgccg acggcacgcc ggtctcgtac
      421 ctgcgcaacg ccgccggcca gccggtctac ggcggcgtcg tcaacatcgg cgggcagtcg
      481 ttcgcggcgc tgaccggcgc cgacttcgcg gcgacgagcg agcgcctgac ccacgtgatg
      541 cagagcatgg ccgtgaagag ccatacgcag ggcccgtggg actgggaact cgacgcaagc
      601 cgctacgact accggcgcga cgacaagcgc cagaacggca tcggcaacgt gctgccggac
      661 gcgctgtatg gcggtgccgg cacgctggcc gacggcagcg gcaccggctg ggtcaacctc
      721 gtggccaagg gcacatggcg cccgcagggc atgaccggcg cgcacatcgt cgacttcggt
      781 gcgcagcacg acgtctacaa gctgcgctac ctcacgtcga acatcgccgg caactggctg
      841 gccgacccgg ccggcacgct ggccagcagt gtcggcggca agacgcaact gcacagcgtc
      901 tacgcgcagg acgcatgggc cttcgccccc cgttggaaga ccgtgctcgg cgtgcgcgcc
      961 gaagactggc gcgcggacga cggcgcgacc gcgttctcgg cgaccagcac gctgcgctac
     1021 ccgtcgcgcc acgagcgcca tctgtcgccg aaggccgcgc tgtcgtacca ggccgcgccc
     1081 gactgggtgc tgaaggccgc cgccggccgc gcgctgcgca tgcccacggt cagcgagctg
     1141 tacggtgcga cctcgaccac caattcgcag tacatcaacg acccgagcct gaagcccgag
     1201 aagtcgtgga ccaccgaact cagcgccgaa cacgacctgg gccatgcgct gctgcgcctg
     1261 acctgcttca gcgagaacac gcacgattcg ctgtacgcgc agacgacgtt cgacccggtc
     1321 gccgacaaga acatcggccg cgtgcagaac gtcgggcgca tgcgtacgaa cgggctggag
     1381 ctcgcgttca acggcagcga cgtgctgcgg cgcgggctgg acttcaacgc cagcgtgacc
     1441 tacgcggatt ccgtcatcaa ggccaacgac ggcttcgtcg tgacgccggg cgacacgctg
     1501 ggcaagcgcc agccgaacat cccgagatgg cgcgccacgg cactcgtcag ctaccgcgtc
     1561 gacgagcgct ggtcggcggc cttcggcgcg cgctacagcg gcacgcaata ccgcacgctg
     1621 aacaacgccg acgtgagcgg cagcacctac cagggcgtca gccggtactt cacgaccgac
     1681 ctgcggctgc gctggcagat cgatcgccaa tggtcggcgg cgttcggcat cgacaacctg
     1741 aacaacgacc ggtactggaa cttccacccc tacccgcagc gcagttacag cgccgaactc
     1801 aggttcgacc tgtgagtcgc cgccacactc acgcccaccc acctcaccga gacccgccat
     1861 gcccttgtcc aagctcctga aactcgccgc ctgttccgcc gtcttcgcgg tcgccagcgc
     1921 gcatgcccac ggcggccacg ccggtgacgt cgagatcacg catcccttcg ccacgccgat
     1981 gcccgccggc gcgagcaacg gcgccgccta tgtcgcgacg ctcgagaaca ccggcaagca
     2041 ggccgaccgg ctgatccgcg tcagcacccc gatcgcgcag cgcgccgaga tccacacgat
     2101 ggcggtcgac gccggcggcg tgatgcgcat gcgcgaggtc ggcgagatcg cgttgccgcc
     2161 cggcgtgctc gtcaagatgc gccccggcga gggttaccat ttcatgctga tggggctgaa
     2221 gcagccgctg aaagagggcg acagcttccc gctgatgctg gagttcgagc acggcggcaa
     2281 ggccgaggtc aaggtcgtcg tgcaggtccc caaggcgcgg cccggcgaga tggccgagca
     2341 caagcactga agttgcggcc tcagcccagc gccgggccgc cccaagtggc ctgagctccc
     2401 ctcgggggac gacgccgcag gcgtcttggg gtcgtcattt cgcgagggcc cgcagcgcga
     2461 gcttgatgcc gtcgctgacg cccacgtcgg gcggcaaagc cttcagtgcc gccgcggcct
     2521 cgcggtcgct gtagccgagc gccagcagcg cctgcaggat gtcgccctgc gcgtggctgg
     2581 cgacgctggt ggtcgtggcg atcgcgtcgc cgagcttgcc cttgagttcg agcagcagcc
     2641 gctcggcggt cttcttgccg atgccgggca ccttcacgag gcgcgcgctc tcctgcaggc
     2701 cgacggcctg cgcgagctcg ttgacgctca gcccggacag aatcgacagc gcggtgcgcg
     2761 gcccgacgcc ggagatcttc acgagcagcc ggaaggtcgc acgctcgtcg tgcgtcagga
     2821 agccgaacag cacctgcgcg tcctcgcgca cgacgaagtg ggtcagcaag gtgacgcgtt
     2881 cgcccaggcc cggcaggttg tagaagctgc tcatcggcac gtcgacttcg tagccgacac
     2941 cgttcacgtc gatgagcagg tgcgggggcg atttctccgc gagcgtgccg gtgagtcttc
     3001 cgatcatcgc cgcattatcc gcggcgcgcc gtggctcaag cgcggcggaa caggcccagg
     3061 cgctgcgcct cgagcgcggc ttccgcgcgc gaactcacgt tgagcttgcg gtagatctgc
     3121 ttgacgtagt cggcgatcgt gtggcgcgac aggttcagct gcacgccgat ctcgggcagc
     3181 gtgaagcctt tcgcgacgcg cagcagcacc tcgttctcgc ggtcggtgag cgcgacgtgc
     3241 ggcatgttct gcgggcgctc ctgcgggcgc gtctgcgcgg cgaagtgcga gatcacgcgg
     3301 cgcgcgatcg acgggctcag cggcggttcg ccctggctca tgcggttgag ctgctcggcg
     3361 atcagctcgc gcgcctgctc cttcagcagg tagccgaacg cgccggcctg cagcgccggg
     3421 aacaggtggt cgtcgtcatc gtggatcgtc accaccaccg actggcattc ggactgcgcc
     3481 tcgcgcagcg ccgcgaccac gtccacgccc gagccgtcgg gcaggccgag gtcgaccagc
     3541 gcgagcttga acttgaccgc cgtcaccagc gcgagcgcgt cgtgcacgcg cgcggcttcg
     3601 tggatctggt cgttcggaaa cacttgtttg atcagcacct tgagccagga gcggatctcg
     3661 ggcaggtctt cgagcagcag gatgttgtcc atcgggacac ctcagggtca atgcgccggg
     3721 gaagggcgcc gcgcgaaagc cgcgcgctcg atcatcgtag tcgggtatcg gcagcgcccc
     3781 ggtcgactgt agcccgcctc acaatgggag cgtgagcctt atcacggtgc catagccggc
     3841 gcccgattcg accaggcact ggccctgcat ctgcttcgca cggtgcttca tgctggccat
     3901 gccgtggccg cggtcgagct tgccgtcgag ctccatcggg atgcccttgc cgttgtcctg
     3961 caccgtcagg ccgaactggc gctcgctgat cgcgcagcgc accttgcagt gcgacgcacc
     4021 gctgtgcttg atgatgttgc tgacggcctc gcgcaggatg cgcgtggtct gcacgaagcc
     4081 gcgcgccggc agcagctgct cgatctcttc ggtcgggccg cgccagtcgg cctcgatgtt
     4141 ggcctggccc aggcgcgaga cggtctcggc gcgccagtcg gccagcgcat cggccagccg
     4201 catcggcttg ccggtcaggc cgcgcaccga caggcgcatc tcttcgagcg cctcgcgcgc
     4261 cagcgtcgag atgcgctccg attcgctggt gtgcacgatc gtcagcagct tcgcgccgag
     4321 gtcgtcgtgc aggtcggcgg cgatgcgctt gcgctccttc tcggtgacct gctcgacgcg
     4381 cagctcggcg agctgcgcga agttgcgctc gatctcggcg gtgatctcgt ggacccggct
     4441 ctcgaggttc tcgcgcccgg cctcggcggc gcgcagcgca cgcgcgaaga cctgcagcag
     4501 ccgcatcgcc agcgcgatga acaccagcgg cagcgcgatc tgcagcagcg gcacgcccag
     4561 cggcggcagc aggtcgtgct ggcgcgcgaa ctcggcgccg agcagcgcga ccaccgcgcc
     4621 gagcagcgcc gccatcgacc agaagtcctg gcgctggccg cgccaggtca cgaccaggta
     4681 cagcacggcc gcgccgacga cctcggccgc gagcgcggcg taccagatgc tggccatcag
     4741 gaacaggcgc ccgggcccgg ccagcaggag cgtggccggc agcagcaccc actgcgcgac
     4801 cagcgcattc tcgatccagc gcgagcgcag gcctgcgaag ctgagcagga actgcaccgc
     4861 gagtgcgacc agcggcgcga agccg
//
LOCUS       DATMJB010000383         5427 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_329124, whole genome shotgun sequence.
ACCESSION   DATMJB010000383 DATMJB010000000
VERSION     DATMJB010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329124"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..528)
                     /locus_tag="VNU48_08300"
     CDS             complement(106..528)
                     /locus_tag="VNU48_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWI11314.1"
                     /translation="MKILLAVDGSDYTKRMLSYIAAHDELLGPGHEYVVLTVVPPVPQ
                     YAARFLERSTLDGFYAEQAELVLKPVRAFATQQRWHVAALHLHGHPAEVIAAHAVSSN
                     ADLIVMGTHGHSALTSLVLGSVASGVLARCTSPVLLIR"
     gene            complement(705..905)
                     /locus_tag="VNU48_08305"
     CDS             complement(705..905)
                     /locus_tag="VNU48_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11315.1"
                     /translation="MTNVQIASNPFALMMDPEAVFAALASSDRLDRLKSRVCRPLDKP
                     HVVRPEGEPPAPDRDAPEAAQD"
     gene            complement(1012..1608)
                     /locus_tag="VNU48_08310"
     CDS             complement(1012..1608)
                     /locus_tag="VNU48_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HWI11316.1"
                     /translation="MLAVFFASAGSFEDFFAAIRNDNASTLTNLLERGFDPNTRDPKG
                     QPGLTMAIQAQSPKAEKALLDRPDIDVNALNEAGESALMMAALKGNLPAMRRLLERGA
                     RVNQPGWSALHYAATGPDPQTVQLLLDRGADIEAASPNGSTPLMMAAQYGSDASVELL
                     LKRGADAKKRNQLNLAAVDFAKLSGRDPLVRRLEQAQR"
     gene            complement(1649..2473)
                     /locus_tag="VNU48_08315"
     CDS             complement(1649..2473)
                     /locus_tag="VNU48_08315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWI11317.1"
                     /translation="MYVDSHCHLSFPELAERIGDIRAAMAAAQVDRALCICTTLEEFE
                     QVHALATRYDNFWCSAGVHPDNEGVREPTVDDLLTLAALPRVVAIGETGLDYYRLNGR
                     SIEAMAWQRERFRVHIRAARAAGLPLVVHTRSASDDTIAILREEGAAGSNAATPGDGP
                     GGVFHCFTETEAVARAALDLGFHISFSGILTFKTAQALRDVAAFVPLDRCLIETDSPY
                     LAPVPYRGKTNTPAYVPFVAQQIAALKGLSVEAVARATSANFERLFRLPALAPDQE"
     gene            complement(2511..2909)
                     /locus_tag="VNU48_08320"
     CDS             complement(2511..2909)
                     /locus_tag="VNU48_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWI11318.1"
                     /translation="MSEKPPPRTVPSALAPATSPARPSVIQLVFREKGALYAAYMPVF
                     SEGGLFVPTTREYRLGEDIYLLLSLPDDPQRYPVAGKVGWITPANASGGRTQGVGVRF
                     PSDEKTRGLKLKIEEALGTSISSAKPTQTI"
     gene            complement(2906..3967)
                     /gene="holB"
                     /locus_tag="VNU48_08325"
     CDS             complement(2906..3967)
                     /gene="holB"
                     /locus_tag="VNU48_08325"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HWI11319.1"
                     /translation="MVGPDGVLPLPWIEPVLRRTLQSQRAHALLLHGPQGIGQFELAL
                     TVAQAWLCEAADASARPCGACASCRLVQAHSHPDLQVLLPEALQEPLGWAAADGDDAG
                     SERAGKTRQPSKEIKVEAVRAAVNFAQTTSARGRAKVVVLHPAERMNGVSANTLLKTL
                     EEPPGAARFVLSCAAPDALLPTIRSRCQSVALALPPTEQAGAWLAAQGVAEPAVLLAA
                     AGGQPQEALDWARQGIAAADWLRLPALVAQGSVAGFATWPLARVVDALQKLCHDAACI
                     AAAAAPRYFPAGSLRAGADPAALHAWMRELNRAARHAEHPWNAALMVESLVQQGQRAL
                     AIVGDKGRAARNTSVHFGA"
     gene            complement(3961..4572)
                     /gene="tmk"
                     /locus_tag="VNU48_08330"
     CDS             complement(3961..4572)
                     /gene="tmk"
                     /locus_tag="VNU48_08330"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWI11320.1"
                     /translation="MSGRFITFEGIDGAGKSTHIDALAQRLRAGGASVVCTREPGGTP
                     LAERVREIFLHESADVLTEALLAFAARRDHLRAVIEPALAAGHTVLCDRFTDATFAYQ
                     GGGGGASVAMLTQLEQWVQRGLQPDLTIWFDLPAALAAQRRAQDRPADRFEQRDLPYF
                     ERVRAGYAARMAAAPQRFARIDASLARDAVWLQIDTALKARAW"
     gene            complement(4592..>5427)
                     /gene="mltG"
                     /locus_tag="VNU48_08335"
     CDS             complement(4592..>5427)
                     /gene="mltG"
                     /locus_tag="VNU48_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWI11321.1"
                     /translation="VQAGVQASPLLLYEWFRWSGQARKIRAGSYEIGRGVTPQALLNK
                     MVRGDETLAVVRLIEGWTFRQFRAELAKADALEPMTASMSDEQLMTALGAPPGVSPEG
                     RFHPDTYAYSKGSSDLAVLKRAYRAMQTRLDAAWQERSPDTPLHSADDALVLASIVEK
                     ETGVGADRGLVAGVFVNRLRLGMPLQTDPTVIYGLGTSFDGNLRKRDLLADTPYNTYT
                     RSGLPPTPIAMPGKAALLAAVRPEPTKALYFVSRGDGHSEFSETLAQHNRAVNQYQRK
                     K"
BASE COUNT          801 a         1864 c         1896 g          866 t
ORIGIN      
        1 agccacgaac ggggtgagtt gctttgccgg acctacaggt ggaggtcgtg caagattcta
       61 gtttacataa tatacattgt cgcacaatcg tgccgcccta acgaatcagc gaatcagcaa
      121 taccgggctc gtgcaacgag ccagcacccc gctcgcgacg gagccgagca ccaggctggt
      181 cagcgccgaa tgcccatggg ttcccatcac gatcaagtct gcgttgctgc tcacggcatg
      241 tgcggcgatc acctcggccg ggtgcccatg cagatgcagc gcagcgacgt gccatcgctg
      301 ttgggtcgcg aacgcgcgaa cgggcttcag caccagttcg gcctgctcgg catagaaccc
      361 gtccagcgtg ctgcgctcga gaaaccgcgc cgcgtattgc ggaaccggcg gcacgaccgt
      421 caggacgacg tattcatgcc cgggacccag cagttcgtcg tgcgccgcga tgtagctgag
      481 catgcgcttg gtgtagtcgc ttccatcgac cgccaagagg atcttcatct gcatctcctt
      541 cagtgccgaa gccagatgcc agtgtgcgcc gcgcgccgcg gccgaccttg tcggggatca
      601 acgaatcaca gtccttgcag gtgcgcggga acgcgggctg ccccgccggt tcgcgcggcg
      661 ggcgagcccc accggccgca ccaagcggcc gatcgtcggt ctgttcagtc ctgcgctgcc
      721 tcgggcgcgt cgcggtcggg cgcgggcggc tcgccttcgg gtcggacgac gtgcggcttg
      781 tcgagcggcc ggcacacccg gctcttcagg cggtccaggc ggtcggaact cgccagcgcg
      841 gcgaacacgg cttccgggtc catcatcagc gcgaacgggt tggaagcaat ctgaacattc
      901 gtcatggcaa tccttccggt ggtcgatgcg atcaactcta tggcgagtct cgcttccacc
      961 ccagtgggtg cgcatccaaa ccggctgtcg ggattcttcc tacgcggcgc gtcagcgctg
     1021 cgcctgctcg aggcgcctca cgagcgggtc gcgccccgac aacttcgcga agtcgaccgc
     1081 cgccaggttg agctggttgc gcttcttcgc gtcggcaccg cgcttgagca gcagttcgac
     1141 gctggcatcg ctgccgtact gcgcggccat catcagcggc gtgctgccgt tcggcgacgc
     1201 cgcctcgata tccgcgccgc ggtcgagcag cagctgcacc gtctgcgggt cgggtccggt
     1261 cgccgcatag tgcagcgccg accagccggg ctggttgacc cgggcgccgc gctcgagcag
     1321 gcggcgcatg gcgggcaggt tgcccttcag tgcggccatc atcagcgccg attcgcccgc
     1381 ttcattgagt gcgttgacgt cgatgtcggg tcggtccagc agcgctttct cggccttcgg
     1441 cgactgcgcc tggatcgcca tcgtcaaccc cggctggcct ttcggatcgc gcgtgttggg
     1501 gtcgaagccg cgctccagca ggttcgtcag cgtgctggcg ttgtcgttgc ggatggcggc
     1561 gaagaaatcc tcgaaggatc cggcactcgc aaagaaaact gcaagcatga caaccgtata
     1621 gacggtatat ctaaagtatt tcatgaggtc attcctggtc tggagccagc gccggaagcc
     1681 tgaaaagccg ctcgaagttt gccgacgtgg cgcgcgcgac cgcctcgacc gacaggccct
     1741 tcagcgcggc gatctgctgc gcgacgaacg gcacgtaggc cggggtgttg gtcttgccgc
     1801 ggtacggcac cggtgcgaga tacgggctgt cggtctcgat caggcagcga tccagcggga
     1861 cgaacgccgc cacatcgcgc agcgcctgcg cggtcttgaa ggtcaggatg cccgagaacg
     1921 agatgtggaa accgaggtcg agcgccgcgc gtgccacggc ctcggtctcg gtaaagcaat
     1981 ggaacacgcc acccgggccg tcgccaggcg tggcggcatt cgagccggca gcgccctcct
     2041 cgcgcaggat cgcgatcgtg tcgtccgaag cgctgcgcgt gtgcacgacc agcggcagcc
     2101 cggcggcgcg tgcggcgcgg atgtgcacac ggaagcgttc gcgctgccat gccatcgcct
     2161 cgatgctgcg gccgttgagg cggtagtagt ccagcccggt ttcgccgatc gcgacgaccc
     2221 gtggcagcgc ggccagcgtc agcaggtcgt cgacggtcgg ttcgcgcacg ccttcgttgt
     2281 ccggatggac gccggcactg caccagaaat tgtcgtagcg cgtcgcgagc gcgtgcacct
     2341 gctcgaactc ctcgagcgtg gtgcagatgc acagcgcacg gtcgacctgc gcggcggcca
     2401 tcgcggcgcg gatgtcgccg atgcgctcgg ccagctccgg aaagctcagg tggcaatggg
     2461 aatcgacgta catgtgcggg cctcgtcgtg cggatggcga cgaagcggct tcagatcgtc
     2521 tgcgtcggct tggcggatga aatcgaggtg cccagcgctt cttcgatctt gagcttcagg
     2581 ccgcgcgtct tctcgtcgct cgggaagcgc acgcccacgc cctgggtgcg gccgccggac
     2641 gcgttggccg gcgtgatcca gccgaccttg ccggccaccg ggtaacgctg cgggtcgtcg
     2701 ggcagcgaca gcagcaggta gatgtcttcg ccgagccggt attcgcgcgt cgtcggcacg
     2761 aacagaccgc cctcggagaa caccggcatg taggcggcgt agagcgcacc cttctcgcgg
     2821 aacacgagct ggatcacgct cggccgtgcc gggctcgtcg cgggcgcgag cgcggacggg
     2881 acggttcgcg gcggcggctt ttcgctcatg cgccgaagtg taccgaagtg ttccgcgccg
     2941 ccctgccctt gtcgccgacg atcgccagcg cgcgctgacc ctgctgcacc agcgactcga
     3001 ccatcagagc ggcgttccac gggtgttcgg cgtggcgcgc ggcccggttg agttcgcgca
     3061 tccatgcgtg cagcgccgcg gggtccgcac cggcgcgcaa cgacccggcc ggaaagtagc
     3121 gcggcgccgc ggccgcggcg atgcaggcag cgtcgtgaca cagcttctgc agcgcatcga
     3181 cgacgcgcgc cagcggccat gtcgcgaagc ccgccaccga accctgagcg acgagcgcgg
     3241 gcaggcgcaa ccaatcggcc gcggcgatgc cctgccgcgc ccagtcgagc gcttcctgcg
     3301 gctggccgcc ggcggcggcg agcagcacgg ccggttcggc cacgccctgc gcggccagcc
     3361 acgcgccggc ctgttcggtg ggcggcaacg cgagtgcgac gctctggcag cggctgcgga
     3421 tcgtcggcag cagcgcatcg ggtgcggcac agctcagcac gaagcgcgcg gcgccgggcg
     3481 gctcctcgag cgtcttcagc aaggtgttgg ccgagacgcc gttcatgcgc tcggccgggt
     3541 gcagcacgac gaccttggcg cggccccggg ccgaggtcgt ctgcgcgaag ttgacggcgg
     3601 cgcgcaccgc ttcgaccttg atttccttgc tcggctgcct ggtcttgccg gcgcgctcgg
     3661 aacccgcgtc gtcgccgtcg gccgcggccc agccgagcgg ctcttgcagg gcctcgggca
     3721 acagcacctg caaatccggg tgcgagtgcg cctgcaccag ccggcagctc gcacacgcgc
     3781 cgcacggccg tgccgacgca tcggccgcct cgcacagcca ggcctgcgcg accgtcagcg
     3841 ccagctcgaa ctggccgatg ccctgcgggc cgtgcagcag cagcgcgtgg gcgcgctggg
     3901 actgcagcgt gcggcgcaac accggctcga tccacggcag cggcagcaca ccgtcggggc
     3961 ctaccatgcg cgggctttca gtgcggtgtc gatctgcagc cagacggcgt cgcgcgccag
     4021 gctggcgtcg atgcgggcaa agcgctgcgg ggccgccgcc atgcgcgccg cgtaaccggc
     4081 gcgcacgcgt tcgaagtacg gcagatcgcg ctgctcgaaa cggtcggccg ggcggtcctg
     4141 cgcgcggcgc tgcgcggcga gcgcggccgg cagatcgaac cagatcgtca ggtcgggctg
     4201 cagaccgcgc tgcacccatt gctcgagctg tgtcagcatc gccacgcttg cgccgccccc
     4261 gccgccctgg tatgcgaagg tcgcatcggt gaagcgatca cacagcaccg tgtggccggc
     4321 cgccagcgcc ggctcgatga ctgcgcgcag gtgatcgcga cgcgcggcaa acgcgagcag
     4381 ggcctcggtc agcacgtctg ccgattcgtg caggaagatc tcgcgcaccc gttcggccag
     4441 cggcgtgccg cccggctcgc gcgtgcagac gacgctcgcg ccgcccgcgc gcaaccgctg
     4501 cgcgagcgcg tcgatgtgcg tcgacttgcc ggcgccgtcg atgccttcga aagtgatgaa
     4561 tctgccgctc acgcgcctgc cctgtcccgg ttcatttctt gcgctggtac tggttcaccg
     4621 cgcgattgtg ctgggccaga gtttcgctga attcgctgtg gccatcgccg cgcgagacga
     4681 agtacaaggc cttggtcggc tcgggccgca ccgcggcgag cagcgcggcc ttgccgggca
     4741 tcgcgatcgg tgtcggcggc aggccgctgc gcgtgtaggt gttgtagggc gtgtcggcaa
     4801 gcaggtcgcg cttgcgcagg ttgccgtcga agctcgtgcc caggccgtag atcacggtcg
     4861 gatcggtctg cagcggcatg cccaggcgca gccggttcac gaacacgccc gcgaccaggc
     4921 cacggtcggc accgacgccg gtttccttct cgacgatcga cgcgagcacg agcgcgtcgt
     4981 cggcgctgtg cagcggcgtg tcgggtgagc gctcctgcca cgcggcatcg agccgtgtct
     5041 gcatcgcccg ataggcccgc ttgagcaccg cgaggtcgct gctgcccttg ctgtaggcgt
     5101 aggtgtcggg gtggaagcgc ccttcgggcg agacgccggg cggcgcgccg agcgcggtca
     5161 tcagctgctc gtcgctcatc gaagcggtca tcggctcgag cgcgtcggcc ttcgccagtt
     5221 cggcgcgaaa ctggcggaag gtccagcctt cgatcaggcg cacgacggcc agcgtttcgt
     5281 cgccacgcac catcttgttg agcagcgctt gcggcgtcac gccacggccg atctcgtagc
     5341 tgccggcgcg gatcttgcgc gcctggcccg accagcgaaa ccattcatag agcagcagcg
     5401 gcgaggcctg cacacccgcc tggaccc
//
LOCUS       DATMJB010000384         4858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_119811, whole genome shotgun sequence.
ACCESSION   DATMJB010000384 DATMJB010000000
VERSION     DATMJB010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119811"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1244
                     /locus_tag="VNU48_08340"
     CDS             132..1244
                     /locus_tag="VNU48_08340"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWI11322.1"
                     /translation="MKMLYLTFNAIDDRSYGAALRSAHIRDAMTQIGQVHTLIIHAGA
                     RMQIDADWDESGVKRATYNRIGLSVGARRQRAEIRAWVGDVLRQGRYDAIVARYLGMA
                     RFVPRWAWPRMVLDADDLVKSVAPDDRVPLRTRLALWARNLVARRVAERAAHVWCVNP
                     LDAARLGTTRVSLLRNVVRIPPANRPRADAVPGRLLMVGLFEYAPNAQALRWFAQAVL
                     PALRAEIPGVELHAVGKCPPALQAELAGQGLHCRGFVTDLAREYDLASLVIAPVQSGG
                     GTQIKVIDALAHQRPLAISAFAHAGFAADLKHPDHLLRCTTAQDWISHCRWALRERAA
                     AEAMADRGYAAVRAYGSDTLTPAIQATLQAVSRQSP"
     gene            complement(1270..2724)
                     /gene="dacB"
                     /locus_tag="VNU48_08345"
     CDS             complement(1270..2724)
                     /gene="dacB"
                     /locus_tag="VNU48_08345"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HWI11323.1"
                     /translation="MSKLPTLAACAALLAVVHAGAAPSSAAALPPAVARAIAASGLPP
                     KSFGLYAQEVIGANTVLALNAEQPYTMASTTKIVTSLAALDLLGPYYRWHTSAFTLGT
                     LADGRLAGDLLIVGGGNAQLTSGELAAWFRRMHDQGLREIDGDIVLDRYAFQLRPSDH
                     AGTPVQSADEPRHVWPDAMTLDEGLIGVVIRPARGPRAALSLTPALSDVTLDNRVASG
                     GACSATPRWQAGRGGHATVIVQGHWGAACGTRTVAFVAPPDSGIAARALPAMWAATGG
                     VLRGRVVAAKVVPGISPIPLGPDGEALAPLSFDRSKPLTELVRDINKVSDNVGARNLM
                     LSLSPGFPTRPATLAGAQRTVRLWLREQGLGDADVEVENGSGLSHSERAKPRALVQLL
                     RKAWRADQAQAFFDSLPVAGIDGTLKNRLLGGHAAGQAYLKTGTLNDTRALAGYVHAA
                     SGKMYAVALMVNHADPARGRPALDAMVEWLARNG"
     gene            2846..3565
                     /gene="trmB"
                     /locus_tag="VNU48_08350"
     CDS             2846..3565
                     /gene="trmB"
                     /locus_tag="VNU48_08350"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HWI11324.1"
                     /translation="MSDPTATPAPPARRSIRSFVVRAGRIGPGQTRALAELGPRFVLP
                     YAAAPVDFAASFGRAAPRVLEIGFGMGDATAAIAQALPGTDFIGVEVHTPGVGALLKQ
                     IGARGLTNLRLVQHDAVDVLQQMIAPGSLSGVHVFFPDPWHKKKHHKRRLIQPEFVRL
                     LAERLAPGGYLHCATDWQPYAEQMLQVLAAEPALRNTVDGYAPRPDYRPLTKFENRGL
                     KLGHGVWDLVFVKPAGTRDAR"
     gene            complement(3714..4550)
                     /locus_tag="VNU48_08355"
     CDS             complement(3714..4550)
                     /locus_tag="VNU48_08355"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWI11325.1"
                     /translation="MDLILLFKAAVMGVVEGVTEFLPVSSTGHLILAGSLIGFTDETV
                     KSAKLFEIVIQAGAIVAVMIVYWQRLREALVGLRGADARQRRFVLNVLIGFLPAAILG
                     VLFSKAIKAHLFTPAVVAATFILGGFVILWAERRQARAVRIQTVDELTPLDALKVGLA
                     QSLALVPGTSRSGATIIGGMLFGLSRKAATDFSFFLAIPTLIGASVYSLWKERAALSA
                     ADGPLLVVGTVVSFAVAWIVVRWLLRYIATHSFVPFAWYRIAFGIVVLLTWWQGWIHW
                     AE"
     gene            4685..>4858
                     /locus_tag="VNU48_08360"
     CDS             4685..>4858
                     /locus_tag="VNU48_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI11326.1"
                     /translation="MKSTPAAHSAAIGPASTRQRRKEARPQELLDAALELFVERGFAA
                     TRSEDVAARAGVSK"
BASE COUNT          702 a         1774 c         1734 g          648 t
ORIGIN      
        1 tgggaagctg ccgttctacc attgaactac acccgcgaac ggtgcggatt ctagcgtggt
       61 ggtcacgctg tcgcgggcac aggccgaggc acggctgggc gcgcgcgcaa cgccaatggc
      121 atcatggccc gatgaagatg ctctacctga ccttcaatgc gatcgacgat cgctcctatg
      181 gcgccgcctt gcgcagcgcg cacatccgcg acgcgatgac gcagatcggc caggtgcaca
      241 cgctcatcat ccacgccggt gcgcggatgc agatcgacgc cgactgggac gaatccggcg
      301 tgaaacgggc gacgtacaac cgcatcggcc tttccgtcgg cgcgcggcgc cagcgcgccg
      361 agatccgcgc ctgggtcggc gacgtgctgc gccaggggcg ctacgacgcg atcgtcgcgc
      421 gctacctcgg catggcgcgg ttcgtgccgc gctgggcgtg gccgcgcatg gtgctggatg
      481 cggacgatct ggtgaagtcg gtcgcgcccg acgatcgggt cccgctgcgc acgcggctcg
      541 cgctgtgggc gcgcaacctg gtcgcgcggc gcgtcgcgga gcgtgccgcg cacgtgtggt
      601 gcgtcaaccc gctggacgcc gcacggctgg gaacgacgcg cgtgtcgctg ctcagaaacg
      661 tggtccggat tccgccggcg aaccggccgc gcgccgacgc ggtgcccggc cggctgttga
      721 tggtgggcct gttcgagtac gcgccgaatg cgcaggcgct gcgctggttc gcgcaggccg
      781 tgctgccggc gctgcgggcc gagatcccgg gcgtcgagct gcacgcggtc ggcaagtgcc
      841 cgccggcgct gcaggccgaa ctggccggcc agggcctgca ctgccgcggc ttcgtgaccg
      901 acctggcccg cgaatacgac ctcgcgtcgc tcgtgatcgc gccggtccag tcgggcggcg
      961 gcacgcagat caaggtcatc gatgcgctcg cgcaccagcg cccgctggcg atctccgcgt
     1021 tcgcgcacgc cggtttcgcg gccgacctga agcacccgga tcacctgttg cgctgcacga
     1081 ccgcgcaaga ctggatcagc cattgccgct gggccctgcg ggaacgcgcc gccgccgagg
     1141 cgatggccga tcgcggctat gcggcggtcc gcgcctacgg cagcgacacg ctgaccccgg
     1201 ccatccaggc cacgctgcag gccgtgagcc ggcagtcgcc gtagcggggc gccggccgcg
     1261 atcgcgccgt cagccgttgc gggccagcca ctcgaccatc gcgtcgagcg cgggccgacc
     1321 ccgcgccggg tcggcgtggt tgaccatcag cgcgaccgcg tacatcttgc cgctcgccgc
     1381 gtgcacatag ccggccagcg cgcgcgtgtc gttcagcgtg ccggtcttca ggtaggcctg
     1441 cccggcggcg tggccgccga gcaggcggtt cttcaaggtg ccgtcgatgc cggccaccgg
     1501 cagcgaatcg aagaaggcct gcgcctgatc ggcgcgccag gccttgcgca gcaactgcac
     1561 cagcgcgcgc ggcttggcgc gctcgctgtg cgacaggccc gagccgttct cgacctcgac
     1621 atcggcgtcg cccaggccct gctcgcgcag ccacagccgc acggtgcgct gcgcgccggc
     1681 gagcgtcgcg ggccgcgtcg ggaagcccgg cgacagcgac agcatcaggt tgcgcgcgcc
     1741 gacgttgtcg ctgaccttgt tgatgtcgcg caccagttcg gtcagcggct tcgagcggtc
     1801 gaacgacagc ggtgccagcg cctcgccgtc cggcccgagc gggatcgggc tgatgccggg
     1861 cacgaccttc gccgccacca cgcgaccgcg cagcacgccg ccggtggcgg cccacatcgc
     1921 cggcagcgcg cgcgcggcga tgccgctgtc gggcggcgcc acgaaggcga cggtgcgcgt
     1981 gccgcaggcg gcgccccaat ggccttgcac gatcaccgtg gcgtggccgc ctcggccggc
     2041 ctgccagcgc ggcgtcgcgc tgcaggcgcc gccgctggcg acgcggttgt ccagcgtcac
     2101 gtcgctgagc gccggcgtca gcgacagcgc ggcgcgtggc ccgcgcgccg ggcggatcac
     2161 gacgccgatc agcccttcgt cgagcgtcat cgcgtcgggc cagacgtggc gaggctcgtc
     2221 ggccgactgc accggcgtgc ccgcatgatc ggacggccgc agctggaacg cgtagcggtc
     2281 gagcacgatg tcgccgtcga tctcgcgcag gccctggtcg tgcatgcgcc ggaaccacgc
     2341 cgccagttcg cccgacgtga gctgcgcgtt gccgccgccg acgatcagca ggtcgccggc
     2401 gagccggccg tcggccagcg ttccgagcgt gaatgccgag gtgtgccagc ggtagtacgg
     2461 gccgagcagg tcgagcgcgg ccagcgaggt gacgatcttg gtcgtcgacg ccatcgtgta
     2521 cggctgctcg gcgttcagcg ccagcaccgt gttcgcgccg atcacctcct gcgcgtacag
     2581 gccgaagctc ttcggcggca ggcccgatgc ggcgatcgcg cgcgccacgg ccggcggcag
     2641 cgccgcggcg gatgacggcg ccgcgccggc gtgcacgacc gcgagcagcg cagcgcaggc
     2701 ggccagggtc gggagcttgc tcatggccgc gaggctagca gcggcaggcc cgcggccgca
     2761 cccccgcacc tgcgtggtcg aacgcccgcg tcgggtcttg gcttgcgcgg cgtcacaatc
     2821 gttgcacctc tgacatgctt tcccgatgag cgacccgacc gcgacccccg caccacccgc
     2881 gcgccgcagc atccgcagct ttgtcgtgcg cgccggccgc atcgggccgg gccagacgcg
     2941 cgcgctggcc gaactcggcc cgcgcttcgt gctgccctac gctgccgcgc cggtcgactt
     3001 cgccgcgagc ttcggccgcg ccgcgccacg ggtgctcgag atcggcttcg gcatgggcga
     3061 cgcgaccgcc gcgatcgcgc aggccctgcc cggcaccgac ttcatcggcg tcgaggtcca
     3121 cacgcccggg gtgggcgcac tgctcaagca gatcggcgcg cgcgggctga cgaacctgcg
     3181 gctggtccag cacgacgcgg tcgacgtgct gcagcagatg atcgcgccgg gttcgctgag
     3241 cggcgtgcac gtcttcttcc cggacccctg gcacaagaag aagcaccaca agcgccgcct
     3301 gatacagccc gagttcgtgc gcctgcttgc cgagcgcctg gcgccgggcg gctacctgca
     3361 ctgcgcgacc gactggcagc cgtatgccga gcagatgctg caggtgctgg cggccgagcc
     3421 ggcgctgcgc aacaccgtcg acggctacgc gccgcggccc gactaccggc cgctgacgaa
     3481 gttcgagaac cgcgggctca agctcggcca cggcgtgtgg gacctggtgt tcgtcaagcc
     3541 ggccggcacg cgcgatgcca ggtagcggcc gatcctggcg acgcgggtcg catcgattca
     3601 tgcactccat caccccatcg accccggcaa caagcccctg cgcgagacgg tcgaggactg
     3661 gcacggcctg gccgcgagcg ggtcgcgcgt gccgggcccg gccccgcgcg ggttcattcg
     3721 gcccagtgga tccagccctg ccaccaggtc agcagcacga cgatgccgaa ggcgatccgg
     3781 taccatgcga acggcacgaa gctgtgcgtg gcgatgtagc gcagcagcca gcgcacgacg
     3841 atccacgcga ccgcgaacga gacgaccgtg ccgaccacga gcagcggccc gtcggcggcc
     3901 gacagcgccg cgcgctcctt ccacaggctg tagacgctgg caccgatcag ggtcgggatc
     3961 gcgaggaaga agctgaagtc ggtggcggcc ttgcggctca ggccgaacag catgccgccg
     4021 atgatggtgg cgcccgaccg gctcgtgccc ggcaccagcg cgaggctttg cgcgaggccg
     4081 accttcagcg catcgagcgg cgtcagttcg tcgacggtct ggatgcgcac cgcgcgcgcc
     4141 tggcggcgct cggcccacag gatcacgaag ccgccgagga tgaaggtcgc cgcgaccacg
     4201 gccggcgtga acaggtgcgc cttgatcgcc ttgctgaaca gcacgcccag gatcgccgcg
     4261 ggcaggaagc cgatcagcac gttgagcacg aagcggcgct ggcgcgcgtc ggcgccgcgc
     4321 agcccgacca gcgcctcgcg cagccgctgc cagtagacga tcatcaccgc gacgatcgcc
     4381 ccggcctgga tcacgatctc gaacagcttg gccgacttga ccgtctcgtc ggtgaagccg
     4441 atcagcgagc ccgcgaggat caggtggccg gtgctggaca ccggcaggaa ttcggtgacg
     4501 ccctcgacga cgcccatgac cgcggctttg aacagaagaa tgagatccaa gaagcgagac
     4561 tccgggcgaa tggacgaatg ggcggacccg cgatgttagc gatgcccgac acacccgaga
     4621 cttgaggccg ccgcgccttg accctcgcgg cacccgccgg tacattactg actcgtcagt
     4681 cagtatgaaa tccacgcccg ccgcccactc cgctgcgatc ggccccgcct cgacgcggca
     4741 gcgccgcaag gaggcccgac cgcaggagct gctggacgcg gcactcgagc tcttcgtcga
     4801 gcgcggcttt gccgccacgc gttccgaaga cgtggccgcg cgcgccggcg tctccaag
//
LOCUS       DATMJB010000385         3132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_244833, whole genome shotgun sequence.
ACCESSION   DATMJB010000385 DATMJB010000000
VERSION     DATMJB010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244833"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..879)
                     /locus_tag="VNU48_08365"
     CDS             complement(115..879)
                     /locus_tag="VNU48_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWI11327.1"
                     /translation="MSAPPGALYLVPNTLDFGTDAATGPIEDVLPAAVLRTAARLTHW
                     VAENARTTRAFLKRVDAIVPLAQPLQAVAIVELPRPAKGTHGGAVDLTALLDPARQGH
                     DIGLISEAGLPGVADPGAALVQAAHTAGVEVIALAGPNSLVLALAASGLNGQSFAFVG
                     YLPVDASARAARIRELEALSRRIGQTQLMIETPYRNEALLGALLANLQPATRVSVSCG
                     LTLPSGFTRSDDVRGWKARPVKLPADVPVVFSMLAG"
     gene            complement(876..1481)
                     /locus_tag="VNU48_08370"
     CDS             complement(876..1481)
                     /locus_tag="VNU48_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HWI11328.1"
                     /translation="MSTRPPLILASTSRYRRELLERLRLPFEVRAPEVDETPRPREWP
                     AALAQRLALAKARAVSAAYPDAVVIGSDQVADLNGEPIGKPGTHERAVAQLRAMRGRS
                     VVFQTAVAVVRASTGYVGTALAPVTVRFRALTDAEIEHYLRAEQPYDCAGSAKCETLG
                     IALLEAIDSDDPTALVGLPLIRTCALLRAAGIDPLAAPPTR"
     gene            1550..2098
                     /locus_tag="VNU48_08375"
     CDS             1550..2098
                     /locus_tag="VNU48_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HWI11329.1"
                     /translation="MNSRDFDPFRLDVAAFAKAAGQLDGRWPLAPFDRLTESAVAAAG
                     GLDAGVVSWSARGESRAMRGGESQVWLHLTAATSVPLECQRCLKPVDTALRLERSFLF
                     VHGEDAAAQLDADSDDDVLALTRALDLRELIEDELLLALPIVPRHEVCPEPLVAAADL
                     EIADEKPNPFAALAALKRTDLN"
     gene            2269..2451
                     /gene="rpmF"
                     /locus_tag="VNU48_08380"
     CDS             2269..2451
                     /gene="rpmF"
                     /locus_tag="VNU48_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HWI11330.1"
                     /translation="MAVQQNKKSPSKRGMHRAHNALNTPGTAIEPTTGENHLRHHISP
                     NGFYRGRKVLKTKADA"
     gene            2584..>3132
                     /locus_tag="VNU48_08385"
     CDS             2584..>3132
                     /locus_tag="VNU48_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HWI11331.1"
                     /translation="MIRVAVDCMGGDHGPSATLPACRAFLAAHPNAELILVGHADALA
                     PAAGWERCRIVAASEVVEMHDAIEVALRRKKDSSLRVAVNQVKVGADGVAAAHACVSA
                     GNTGALMAVSRYVLKTLEGIDRPAIATVLPNEHDGGTTVLDLGANVDCSAEHLLQFAV
                     MGSALVAAVDGKANPSVGLLNIG"
BASE COUNT          442 a         1140 c         1113 g          437 t
ORIGIN      
        1 gcgccagccg cgccgcgcgg cctgtcgccg aacatcaagc cgaagaaacg cgtcgcggcg
       61 ctgttcggcg cgaagcgggc cgatccggtc acgtcgtgac ctcgctccgg cgcttcagcc
      121 ggccagcatc gagaacacca ccggcacatc ggccggcagc ttgacggggc gcgccttcca
      181 gccgcgcacg tcgtcgctgc gcgtgaagcc gctcggcaag gtcagaccac agctgaccga
      241 cacgcgcgtg gccggctgca ggttcgccag cagcgcaccc agcagcgctt cgtttcggta
      301 gggcgtttcg atcatcagct gggtctggcc aatgcggcgc gacagcgcct cgagctcgcg
      361 gatgcgtgcg gcgcgtgcgc tggcgtccac cggcaggtag ccgacgaacg cgaagctctg
      421 gccgttcagg ccgctggccg ccagcgccag caccagcgag ttcggcccgg cgagcgcgat
      481 cacctcgacg cccgccgtat gcgcggcctg taccagcgcg gcgccggggt cggcgacgcc
      541 gggcagcccg gcctcggaga tcaggccgat gtcgtggccc tgccgcgccg ggtcgagcag
      601 cgctgtcaaa tcgacggcgc cgccgtgcgt gccctttgcc ggccgcggca gctcgacgat
      661 cgcgactgcc tgcagcggct gcgccagcgg cacgatggcg tcgacgcgct tcaggaaggc
      721 ccgcgtcgtc ctcgcgtttt cggcgaccca gtgagtcagg cgcgcggcgg tgcgcagcac
      781 cgcggccggc agcacatcct cgatcgggcc ggtcgcggcg tcggtgccga aatcgagtgt
      841 attcggcacc aggtagagtg ccccgggcgg cgcgctcatc gcgtgggcgg cgctgccagc
      901 ggatcgatgc ccgccgcgcg cagcagcgcg caggtgcgga tcagcggcag gccgaccagg
      961 gcggtggggt cgtcggagtc gatcgcctcg agcagcgcga tgcccagcgt ctcgcacttc
     1021 gcgctgccgg cgcagtcgta gggctgttcg gcgcgcaggt agtgttcgat ctcggcgtcg
     1081 gtcagcgccc gaaagcgcac cgtcaccggc gcgagtgccg tgcccacgta gccggtcgac
     1141 gcgcgcacca ccgccaccgc ggtctggaac acgacgctgc gcccgcgcat cgcgcgcagc
     1201 tgcgccaccg cgcgctcgtg cgtgccgggc ttgccgatcg gttcaccgtt caggtcggcg
     1261 acctggtccg aaccgatcac cacggcatcg gggtatgcgg ccgacaccgc gcgcgccttc
     1321 gccagcgcca gccgctgcgc gagcgccgcg ggccattcgc gcggccgcgg cgtttcgtcg
     1381 acctcgggcg cgcgcacctc gaacggcagg cgcagccgct cgagcaattc gcgccggtag
     1441 cgcgaggtcg aggccaggat caacggcggt cgcgtgctca tgcggcgatt gtcccatcgc
     1501 ggcgcggcgc gtagcccgag acgcaactcc tgcgccgtac actggcgcca tgaactcgcg
     1561 cgatttcgac ccgttccggc tggacgtcgc ggccttcgcc aaggccgcgg ggcagctcga
     1621 cggtcgctgg ccgctggcgc cgttcgaccg cttgaccgaa tcggccgtcg ccgcggcggg
     1681 cgggctcgac gccggcgtgg tgagctggag cgcgcgcggc gagtcgcgtg ccatgcgcgg
     1741 cggcgagtcg caggtctggc tgcacctgac ggccgccacg tcggtgccgc tcgagtgcca
     1801 gcgctgcctg aagccggtcg acacggcgct gcggctcgag cgcagcttcc tgttcgtgca
     1861 cggcgaggac gccgccgccc agctcgacgc cgacagcgac gacgacgtgc tcgcgctgac
     1921 gcgcgcgctc gacctgcgcg agctgatcga ggacgagttg ctgctcgcgc tgccgatcgt
     1981 gccgcgccac gaggtctgcc ccgagcccct cgtggcggcg gccgaccttg agatcgccga
     2041 cgaaaagccg aatccgttcg ccgccctggc cgcactgaag cgcaccgacc tgaactgaga
     2101 agacatcgcc ccgagccgtg ctagaatcgc gggcttccca gcaggcgtgg agttttttcg
     2161 acgcccggca atattcggaa aaagctgcgt cgcgagcggg tcgttcccct tcgcgcttgc
     2221 cccccggcgc cgtctctcgg gtgagacacc gggcccaagg agaaccccat ggccgtccaa
     2281 cagaacaaga agtccccgtc gaagcgcggc atgcaccgtg cgcacaacgc cctgaacacc
     2341 ccgggcaccg cgatcgagcc gaccaccggc gagaaccacc tgcgtcacca catcagcccg
     2401 aacggcttct accgtggccg caaggtgctg aagaccaagg cggacgcgta aggcgccgcc
     2461 ggctcgattc gccgaccgtt cgcgttgaca cgtgcgcggc ttcgtgaccc gagtctctgt
     2521 agcttgcgct gactcacctg gctcgcgccg cgccgcgatg ccgggcgttc gtctatgagc
     2581 ggcgtgatcc gcgtcgcggt cgactgcatg ggcggtgacc acggcccgtc cgccaccttg
     2641 ccggcctgcc gcgcgttcct ggcggcgcac ccgaacgccg aattgatcct ggtcggccat
     2701 gccgatgccc tggcgcccgc ggcgggctgg gagcgctgcc gcatcgtcgc ggcgagcgaa
     2761 gtcgtcgaga tgcacgacgc catcgaggtc gcgctgcgcc gcaagaagga ctcgtcgctg
     2821 cgtgtggccg tcaaccaggt gaaggtcggt gccgacggtg tcgcggccgc gcacgcctgc
     2881 gtgtcggccg gcaacacggg cgcgctgatg gcggtgtcgc gctacgtgct caagacgctc
     2941 gaaggcatcg accggccggc gatcgccacc gtgctgccga atgagcacga cggcggcacg
     3001 accgtgctcg acctcggcgc caacgtcgac tgctcggccg agcacctgct gcagttcgcg
     3061 gttatgggca gcgcgctggt ggcggccgtc gatggcaagg ccaacccgag cgtggggctg
     3121 ctgaacatcg gc
//
LOCUS       DATMJB010000386         5290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_330320, whole genome shotgun sequence.
ACCESSION   DATMJB010000386 DATMJB010000000
VERSION     DATMJB010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330320"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1911
                     /locus_tag="VNU48_08390"
     CDS             <1..1911
                     /locus_tag="VNU48_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HWI11332.1"
                     /translation="AGIGVLSRTTDPAEYYVAGRRVPALYNGMATGADWMSAASFIGL
                     AGTLYLQGYGGLAYVIGWTGGFVLVAVLLAPYLRKFGQYTIPDFLAARYGGRLPRLIG
                     LLAAILCSFVYLVAQIYGVGLITTQLTGVDFVLGIFLGLGGVLVCSFLGGMRAVTWTQ
                     VAQYIILVTAYVIPVAWLSIKQTGVPVPQLVVGQQLAKVSEREQQLLHDPKELEVRAL
                     FKARADAYANKLKDVPAALVTDRAEAQRRSHELKAANAPLREIQAADKALATQPKTEA
                     LARETWTRARAVDEQRAQPLAGMPLHAQQFAGDPSGDAGQRKTFDESRRNFIALVFCL
                     MVGTAALPHILMRSYTTPSVRAARESVAWSLFFILLLYLTAPALAVLVKFEVFSGLVG
                     LPFDRLPTWIAEWTRVDPSLVSVIDVNHDGILQLGEIRIGSDLVVLATPEIAGLPYVV
                     SGMVAAGGLAAALSTADGLLLTIGNALSHDLYFKLLGPDASAARRVTVSKVLLLVAAL
                     AAAAVAAQRPADILFLVTAAFSIAASAFFPALVLGIFWSRANRWGALCGMVSGLALTL
                     YYLIRNEAWLRGLFRVGAPVDLWFGILPVSAGVFGVPLGFATCVLVSLVTPRPSVRVR
                     EFVLGLRQPGPG"
     gene            2052..2489
                     /gene="rpsF"
                     /locus_tag="VNU48_08395"
     CDS             2052..2489
                     /gene="rpsF"
                     /locus_tag="VNU48_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWI11333.1"
                     /translation="MRHYEIVLLIHPDQSEQIPAMLERYKGVVTAGGGKVHRVEDWGR
                     RQMAYMIQKLAKTHYLCLNIECSKEVLVELETGFRFNDAVIRHLTVLQDEAVTGPSVM
                     MKMVEREDSRKSSPPPDRGDRGDRGDRPERAERQERPPQEASA"
     gene            2524..2805
                     /gene="priB"
                     /locus_tag="VNU48_08400"
     CDS             2524..2805
                     /gene="priB"
                     /locus_tag="VNU48_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HWI11334.1"
                     /translation="MNRLVLTAQLAERGALRYTPAGLPALDLSLKHESEVTQDGKPRK
                     VWFEIRARAIGEITARVEKLELGSSHGFAGFLGSQRNGRGVVFHVTELD"
     gene            2865..3155
                     /gene="rpsR"
                     /locus_tag="VNU48_08405"
     CDS             2865..3155
                     /gene="rpsR"
                     /locus_tag="VNU48_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HWI11335.1"
                     /translation="MPPPRGKGRFSKDRKPKRNTQSLLFRRKRFCRFTVTGVKQIDYK
                     DIDTLRDFIGENGKITPARLTGTRAFFQRQLTTCIKRARFLAMLPYSDQHKV"
     gene            3167..3619
                     /gene="rplI"
                     /locus_tag="VNU48_08410"
     CDS             3167..3619
                     /gene="rplI"
                     /locus_tag="VNU48_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HWI11336.1"
                     /translation="MQIILLEKVANLGNLGDVVKVKDGYARNFLIPQRAARRATEAAI
                     KEFEAKRAELEKAAAEKLATAAALGEKMSGRTVHITQKAGVDGRLFGSVTNADIADAL
                     TRIDFKVVKSQVRMPNGPLKTVGEHTVSVAPHTDVVVEVKVVVLGETD"
     gene            3756..5177
                     /gene="dnaB"
                     /locus_tag="VNU48_08415"
     CDS             3756..5177
                     /gene="dnaB"
                     /locus_tag="VNU48_08415"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWI11337.1"
                     /translation="MSTFLPVEQLSHGSRPDDEVSKLRIPPHSVEAEQSVLGGLLLDN
                     SAWDRAGDLLTDSDFYRFEHRLIYSAIGGLIAATKPADVITVFEQLQGLGKADDCGGL
                     VYLNALAQSVPSAANMRRYAEIVRERAVLRKLVAASDEIATSAFNPQGRNVSEILDEA
                     EGKIFRIGEEGSKSQQGFQSMDTLVVQLLDRVTELHENGAEDVTGVRTGYFDLDKYTS
                     GFQPGDLIVLAARPSMGKTALALNIAEHVAVSEGLPVVVFSMEMGAAQLALRLVGSLG
                     RIDQSNLRTGRLKDDEWTRLSEAVEKLGSVSMFIDESSALTPTEVRARARRQARQCGQ
                     LGMIVVDYLQLMSGSAGGSDENRATVIGEISRGLKSLAKELKCPVLALSQLNRSVESR
                     TDKRPMMSDLRESGAIEQDADVIMFIYRDDYYNKEASKEPGVAEIIIGKQRNGPTGTV
                     KLFFNKPLTKFDNLAPGSAGSDN"
BASE COUNT          866 a         1795 c         1788 g          841 t
ORIGIN      
        1 gcgggcatcg gcgtcctgag ccgcaccacc gacccggccg agtactacgt cgccggccgg
       61 cgcgtaccgg cgctctacaa cggcatggcg accggcgccg actggatgtc cgccgcctcg
      121 ttcatcggcc tggccggcac gctctacctg cagggctacg gcgggctggc ctacgtgata
      181 ggctggaccg gcggcttcgt gctggtggcg gtgctgctgg cgccgtacct gcgcaagttc
      241 ggccagtaca cgattcccga cttcctggcc gcgcgctacg gcggccggct gccgcggctg
      301 atcgggctgc tcgccgcgat cctgtgctcg ttcgtctacc tggtcgcgca gatctacggc
      361 gtcggcctga tcacgaccca gctcaccggt gtcgacttcg tgctcggcat cttcctcggt
      421 ctgggcggcg tgctggtctg ttcgttcctc ggcggcatgc gcgcggtgac ctggacccag
      481 gtcgcgcagt acatcatcct ggtgaccgcc tacgtgatcc cggtggcctg gttgtcgatc
      541 aagcagaccg gcgtgccggt gccgcagctc gtcgtcggcc agcagttggc gaaggtcagc
      601 gaacgcgagc agcagttgct gcacgacccg aaggagctcg aggtgcgcgc gctgttcaag
      661 gcccgggccg acgcctacgc gaacaagctg aaggacgtgc ccgccgcgct cgtcacggac
      721 cgcgcagagg cgcaacggcg cagccacgaa ctgaaggcgg cgaacgcgcc gctgcgcgag
      781 atccaggccg ccgacaaggc gctcgcaacc cagcccaaga ccgaggcgct tgcgcgcgag
      841 acctggacgc gcgcccgggc cgtcgacgaa cagcgtgccc agccgctggc cggcatgccg
      901 ctgcacgccc agcagttcgc cggcgatccc tccggcgacg cgggccaacg caagacgttc
      961 gacgagtcgc gccgcaattt catcgcgctg gtgttctgct tgatggtggg cacggcggcg
     1021 ctgccgcaca tcctgatgcg cagctacacg acgccttcgg tgcgggcggc gcgcgaatcg
     1081 gtggcctggt cgctgttctt catcctgctg ctgtacctga cggcgccggc cctggcggtg
     1141 ctggtcaagt tcgaggtctt cagcggcctg gtcggcctgc ctttcgaccg cctgccgacg
     1201 tggatcgccg agtggacgcg cgtcgacccg tcgctggtgt cggtgatcga cgtcaaccac
     1261 gacggcatcc tgcagctcgg cgagatccgc atcggcagcg acctcgtcgt gctggccacg
     1321 cccgagatcg ccgggctgcc ttacgtggtc tcgggcatgg tcgccgcggg cgggctcgcc
     1381 gcggcgttgt cgaccgccga cgggctgctg ctgacgatcg gcaacgcgct gtcgcacgac
     1441 ctgtacttca agctgctcgg gccggatgcg tcggcggcgc ggcgggtcac ggtctcgaag
     1501 gtgctgctgc tggtcgccgc gctggccgcg gccgcggtgg cggcgcagcg gccggccgac
     1561 atcctgttcc tcgtcaccgc ggcattctcg atcgcggcct cggcgttctt cccggcgctg
     1621 gtgctgggca tcttctggag ccgtgcgaac cgttggggcg cgctgtgcgg catggtgagc
     1681 ggactggcgt tgacgctgta ctacctgatc cgcaacgagg cctggctgcg cgggctgttc
     1741 cgggtcggcg cgccggtcga tctgtggttc ggcatcctgc cggtgtcggc cggcgtgttc
     1801 ggcgtgccgc tcgggttcgc cacctgcgtg ctggtcagcc tggtgacgcc gcgcccgtcg
     1861 gtcagggtgc gcgagttcgt gctcggcctg cgccagccgg gcccgggttg agcgaccatc
     1921 tacccccggt ggcgaagccg gctataatcg tcggctcacc ttgccacacc cgggtctgac
     1981 gcttcggggt ggctcaactg cctcggcccc gcttcatcaa cagcgggccg agcgaaccac
     2041 aaggagtccg aatgcgtcat tatgaaatcg tgctgctgat ccacccggat caaagcgaac
     2101 agattccggc gatgctggaa cgttacaagg gcgtcgtcac cgccggcggt ggcaaggtcc
     2161 accgcgtgga agactggggt cgtcgtcaga tggcctacat gatccagaag ctcgcgaaga
     2221 cgcactacct gtgcctgaac atcgagtgca gcaaggaagt gctggtcgag ctcgaaaccg
     2281 gcttccgctt caacgacgcc gtcattcgtc acctcacggt gctgcaggac gaagccgtga
     2341 ccggcccgtc ggtgatgatg aagatggtcg agcgcgaaga ctcgcgcaag tcgtctccgc
     2401 cgccggatcg tggcgaccgc ggtgaccgtg gtgatcgccc ggagcgtgcg gaacgtcagg
     2461 agcgcccgcc gcaggaggcg tcggcctgac gatgccgtac cgttgccttc gtcgccgcgc
     2521 tgaatgaacc gcctggttct gacggcgcag ctggcggagc gaggtgcgtt gcgatacacc
     2581 cccgccggtt tgccggccct cgatttgagc ctgaagcacg aatcggaggt cacgcaagac
     2641 ggcaagcccc gcaaggtctg gttcgagatc cgggcgcggg cgatcgggga gatcacggcg
     2701 cgcgtcgaga agctcgaatt gggaagcagc cacggctttg ccggcttcct gggctcgcag
     2761 cgcaacggtc gaggtgtcgt gtttcatgta accgagctgg attgaggtct cacggatccg
     2821 atcggctcac tgttcgactc aagcgaaatt gaaagaggtg tcatatgccc ccgccacgtg
     2881 gtaaaggccg gttctcgaag gaccgcaaac ccaagcgcaa tacccagtcg ctgttgttcc
     2941 gtcgcaagcg cttctgccgc ttcacggtca ccggcgtcaa gcagatcgac tacaaggaca
     3001 tcgacacgct gcgcgacttc atcggtgaga acggcaagat cacgcccgcc cgcctgaccg
     3061 gcacgcgtgc gttcttccag cgccagctga cgacgtgcat caagcgcgcg cgtttcctgg
     3121 cgatgctgcc gtacagcgac cagcacaagg tctgaggagc acccgcatgc aaatcatcct
     3181 gctggaaaaa gtcgccaacc tcggcaacct cggcgacgtc gtcaaggtga aagacggcta
     3241 cgcccgcaac ttcctgatcc cgcaacgggc ggcgcgccgc gccaccgaag cggcgatcaa
     3301 ggagttcgaa gcgaagcgcg ccgagctcga gaaggccgcc gccgagaagc tcgccacggc
     3361 cgctgcgctg ggcgagaaga tgagcggccg caccgtgcac atcacgcaga aggccggcgt
     3421 cgacgggcgc ctgttcggct cggtcacgaa cgccgacatc gccgatgcgc tgacccgcat
     3481 cgacttcaag gtcgtcaagt cgcaggtgcg catgcccaat ggtccgctga agaccgtcgg
     3541 cgagcacacc gtgagcgtgg cgccgcacac cgacgtggtc gtggaagtca aggtcgtggt
     3601 gctgggcgaa accgactgac ggttcacgca caacgaccaa gggccggctt cgccggccct
     3661 tccttttttg cgccgtcact tatccaccgc agtcccctgc atttccacag ggttgcgcac
     3721 agccgtggcg acgccacggc cctatgatgc cgccgatgtc gaccttcctg cccgttgaac
     3781 agctctcgca cggctcgcgc cccgacgatg aagtctcgaa actgcgcatc ccgccgcact
     3841 cggtcgaggc cgagcagagc gtgctcggcg gtctgctgct cgacaacagc gcctgggacc
     3901 gcgccggcga cctgctgacc gacagcgact tctaccgctt cgaacatcgc ctgatctaca
     3961 gcgcgatcgg cggcctgatc gccgcgacca agccggccga cgtgatcacc gtgttcgagc
     4021 agctgcaagg gctgggcaag gccgacgact gcggcggcct ggtctacctc aacgcgctcg
     4081 cgcagagcgt accgagcgcg gccaacatgc gccgctacgc cgagatcgtg cgcgagcgcg
     4141 cggtgctgcg caagctggtc gcggccagcg acgagatcgc caccagcgcg ttcaacccgc
     4201 aggggcgcaa cgtctccgag atcctcgacg aggccgaggg caagatcttc cgcatcggcg
     4261 aggaaggctc gaaatcgcag cagggcttcc agagcatgga cacgctggtc gtgcagctgc
     4321 tcgaccgcgt caccgaactg cacgagaacg gcgccgagga cgtgaccggc gtgcgcaccg
     4381 gctacttcga cctcgacaag tacacctcgg gcttccagcc gggcgacctg atcgtgctgg
     4441 cggcgcggcc gtcgatgggc aagaccgcgc tggcgctcaa catcgccgag cacgtggccg
     4501 tcagcgaagg gctgccggtg gtcgtgttct cgatggaaat gggcgccgcg cagctggcgc
     4561 tgcggctggt cggctcgctc ggccgcatcg accagagcaa cctgcgcacc ggccggctca
     4621 aggacgacga atggacccgc ctgtccgagg cggtcgagaa gctcggcagc gtcagcatgt
     4681 tcatcgacga gagctcggcg ctcacgccca ccgaggtgcg cgcccgggcg cgccgccagg
     4741 cgcgccagtg cggccagctc ggcatgatcg tggtcgacta cctgcagctg atgagcggca
     4801 gcgccggcgg cagcgacgag aaccgcgcca ccgtgatcgg cgagatctcg cgcggcctga
     4861 agtcgctggc gaaggaactg aaatgcccgg tgctcgcgct ctcgcagctc aaccgcagcg
     4921 tcgagtcgcg caccgacaag cgtccgatga tgagcgacct gcgcgagtcc ggcgcgatcg
     4981 agcaggacgc cgacgtcatc atgttcatct accgcgacga ctactacaac aaggaagcca
     5041 gcaaggagcc cggcgtcgcc gagatcatca tcggcaagca gcgcaacggc ccgaccggca
     5101 cggtcaagct gttcttcaac aagccgctga cgaagttcga caacctggca ccgggcagcg
     5161 cgggcagcga caactgatgc tggcgcgctg ccgggacggt ccgtcgcagc gggactcaac
     5221 tcgcgtgttc gatcgctggc cgctgcgcgc tgccgttgcg ggactgccgt gacgcacggc
     5281 aaagcggtcg
//
LOCUS       DATMJB010000387         3048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_652832, whole genome shotgun sequence.
ACCESSION   DATMJB010000387 DATMJB010000000
VERSION     DATMJB010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652832"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VNU48_08420"
     CDS             complement(<1..923)
                     /locus_tag="VNU48_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI11338.1"
                     /translation="MAANRPLLEVRNLRVEFPTRRGTLVAVDDISFHIDPGEVLGVVG
                     ESGAGKSMTGAAVIGLIEPPGRIGGGEIRLDGRRIDNLPYEDLRKVRGREIGAIFQDP
                     LTSLNPLYTIGRQLTETITTHLDVSGEEARQRAVALLQEVGIPAAEKRIDHYPHQFSG
                     GMRQRVVIALALCANPRLIIADEPTTALDVSIQAQIITLLKRLCREHGAAVMLITHDM
                     GVIAETADRVAVMYAGRIAEIGPVREVIKNAKHPYTAGLMGSIPKVGRTAARLAQIDG
                     AMPRLNAIPPGCAFNPRCTRVFGRCRVERPEP"
     gene            complement(923..1867)
                     /locus_tag="VNU48_08425"
     CDS             complement(923..1867)
                     /locus_tag="VNU48_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI11339.1"
                     /translation="MATVAPSPVDAPSALRRLLDGDMWWSFRRNPMVVASAAVTALFI
                     VCAIFAPWIAPHDPFDLKTISILDNNNPPAWLEEGEWRFPLGTDDQGRDVLSTILYGS
                     RISLLVGFSAVLFSMTLGVVLGLVAGYVGGITESVIMRAADVKLSFPPILVALLVDGV
                     ARAALKRELHDAIALYVVIFAIGFSVWPQYARTVRGSTLVERNKEYVQAARVIGLHPG
                     LIMLRHVLPNVTGPVLVIATLGLGNAIIAEATLSYLGVGLPATQPSLGTLIRIGNDFL
                     FSGEWWITIFPGAALAILVLAVNLLGDWLRDALNPKLR"
     gene            complement(1870..2856)
                     /locus_tag="VNU48_08430"
     CDS             complement(1870..2856)
                     /locus_tag="VNU48_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI11340.1"
                     /translation="MIAFVIHRLLQSVLVMLAVGFIAFSLFSYVGDPVLIMLGQDYTE
                     QQRITLTRDLGLDRPFFVQFANYMLAAVQGEFGISYRLSRPVDQLILERLPATLELSL
                     VAAVLALALGVPMGVYSALRRDSWLSRIFLTLSLIGVSLPVFLIGILLILIFAVKLGV
                     LPSFGRGDVVRLGRWSTGFLTWSGVRALLLPAVTIALFNMTLIMRLVRAEMLEVLRTD
                     YIKFARARGLSDRAVNFGHALKNTLVPVITIVGLQLGAIIAFAIVTETVFQWPGMGLL
                     FIQSVYFADIPAMAAYLMLIGFFFVVINLVVDLLYYVVDPRLRIDRLAVAGH"
BASE COUNT          534 a         1025 c         1075 g          414 t
ORIGIN      
        1 ggctcgggcc gctcgacccg gcagcgcccg aacacccgcg tgcagcgcgg gttgaaggcg
       61 cagcccggcg ggatcgcgtt gagccgcggc atcgcgccgt cgatctgcgc gagccgcgcc
      121 gcggtgcgcc cgaccttggg gatcgagccc atcagcccgg cggtgtaggg atgcttggcg
      181 ttcttgatca cctcgcgcac cgggccgatc tcggcgatgc gcccggcgta catcaccgcc
      241 acgcggtcgg cggtctcggc gatcacgccc atgtcgtggg tgatcagcat caccgccgcg
      301 ccgtgctcgc ggcagagccg cttgagcagc gtgatgatct gcgcctggat cgagacgtcg
      361 agcgcggtcg tcggctcgtc ggcgatgatc agccgcgggt tggcgcacag cgcgagcgcg
      421 atcaccacgc gctggcgcat gccgccggag aactggtgcg ggtagtggtc gatgcgcttc
      481 tccgccgccg ggatgccgac ctcctgcagc agcgcgaccg cacgttggcg cgcctcctcg
      541 cccgagacgt cgagatgggt ggtgatggtc tcggtcagct ggcggccgat cgtgtagagc
      601 ggattgagcg aggtcagcgg gtcctggaag atggcgccga tctcgcggcc gcgcaccttg
      661 cgcagatcct cgtagggcag gttgtcgatg cggcgcccgt cgagccggat ctcgccgccg
      721 ccgatccggc cgggcggctc gatcagcccg atcaccgccg cgccggtcat cgacttgccc
      781 gcgccggatt caccgaccac gcccagcacc tcgccgggat cgatgtggaa cgagatgtcg
      841 tcgaccgcga ccagcgtgcc gcggcgcgtc gggaactcga cccgcaggtt gcgcacctcg
      901 agcagcgggc ggttggcggc catcagcgga gcttcgggtt gagggcgtcg cgcagccagt
      961 cgcccagcag gttgacggcg agcaccagga tggcgagcgc ggcgccgggg aagatcgtga
     1021 tccaccactc gcccgagaac aagaagtcgt tgccgatgcg gatcagcgtc ccgagcgaag
     1081 gctgcgtcgc cggcagcccg acgccgaggt aggagagcgt cgcctcggcg atgatggcgt
     1141 tgccgaggcc gagcgtggcg atcaccagca ccggccccgt cacgttgggc agcacgtggc
     1201 gcagcatgat caacccggga tgcaggccga tcacccgcgc cgcttgcacg tattccttgt
     1261 tgcgctcgac cagcgtcgag ccgcgcacgg tgcgtgcgta ttgcggccaa accgagaagc
     1321 cgatcgcgaa gatgaccacg tagagcgcga tggcgtcgtg cagctcgcgc ttcagcgcgg
     1381 cgcgggcgac gccgtcgacc agcagcgcga ccaggatcgg ggggaagctg agcttgacgt
     1441 cggcggcgcg catgatgacc gactcggtga tgccgccgac gtagccggcc accagcccga
     1501 gcacgacccc gagcgtcatc gagaacagca ccgcgctgaa gccgaccagc agcgagatgc
     1561 ggctgccgta gaggatcgtc gacagcacgt cgcgaccctg gtcgtccgtc ccgagcggga
     1621 agcgccactc gccctcctcc agccaggccg gcgggttgtt gttgtcgagg atgctgatgg
     1681 tcttgaggtc gaacgggtcg tgcggcgcga tccacggggc gaagatcgcg cagacgatga
     1741 acagcgccgt caccgccgcc gatgccacta ccatcgggtt gcgccggaac gaccaccaca
     1801 tgtcgccgtc gagcaaacgc ctcagcgcgg acggcgcgtc gaccggggag ggagcgacgg
     1861 tggccatgct cagtgtcccg ccacagcgag gcggtcgatc cgcagccgcg gatcgacgac
     1921 gtagtagagc aggtcgacga ccaggttgat gacgacgaag aagaaaccga tcagcatcag
     1981 ataggcggcc atcgccggga tgtcggcgaa gtagaccgac tggatgaaga gcagtcccat
     2041 tcccggccac tggaacaccg tctcggtgac gatcgcgaag gcgatgatcg cgccgagctg
     2101 gagtccgacg atggtgatca ccggcaccag cgtgttcttg agcgcgtggc cgaagttcac
     2161 cgcccggtcg ctcagcccgc gcgcgcgcgc gaacttgatg tagtcggtgc gcagcacctc
     2221 gagcatctcc gcgcgcacca gccgcatgat cagcgtcatg ttgaacagag cgatcgtcac
     2281 cgccgggagc aacagcgcgc gcaccccgct ccacgtcagg aagccggtgc tccagcgccc
     2341 cagccgcacc acgtcgccgc ggccgaacga gggcagcacg ccgagcttca ccgcgaagat
     2401 caggatcagc aggatgccga tcaggaacac cggcagcgag actccgatca gcgacagcgt
     2461 gaggaagatc cggctcagcc aggagtcgcg ccgcaaggcg gaatagaccc ccatcggcac
     2521 gccgagcgcg agcgccagca ccgccgccac caggctgagc tcgagcgtcg ccggcagccg
     2581 ctcgaggatc agctggtcga ccggccgcga cagccggtag gagatgccga actcgccctg
     2641 caccgccgcg agcatgtagt tggcgaactg cacgaagaac ggccggtcga ggccgagatc
     2701 gcgcgtcagc gtgatgcgct gctgctcggt gtagtcctgc ccgagcatga tcagcacggg
     2761 atcgccgacg tagctgaaca gcgagaaggc gatgaagccc accgccagca tgacgaggac
     2821 ggattgcagc aggcggtgga tgacgaaggc gatcatgcgt ggagcggagg caactatcgg
     2881 cccgcctccc gctaagtcaa tggtcgaaag gcgtttcccg gcggtcggcg ggcggggtgg
     2941 cggcgccgtt cggccgccgc tagagtcgcg aaagcatccc tccggagttc acggcatggc
     3001 ccagcgtccc aactcgctcc aggcgcgcga catcgcgtcg ctcgtcca
//
LOCUS       DATMJB010000388         7472 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_809144, whole genome shotgun sequence.
ACCESSION   DATMJB010000388 DATMJB010000000
VERSION     DATMJB010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809144"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VNU48_08435"
     CDS             complement(<1..398)
                     /locus_tag="VNU48_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HWI11341.1"
                     /translation="MISREPTIERLAVARALLLEPFGLTDATLSKALATIATHRIDDA
                     DLYFQYTRSEGWSLEEGIVKAGSFSIDRGVGIRAVHGEKQAFAYSDDISLVALNEAAV
                     AARAIGRQGQSAVAALSRVGSAHSLYAPADP"
     gene            complement(483..1376)
                     /gene="rsmI"
                     /locus_tag="VNU48_08440"
     CDS             complement(483..1376)
                     /gene="rsmI"
                     /locus_tag="VNU48_08440"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWI11342.1"
                     /translation="MLQAAAATAGAQHYPTGALYVVATPIGNLADITLRAIHVLALVD
                     AVACEDTRHSAPLLRQLGIEGKALIALHQHNEREAAAGVLLRLARGERVAYVSDAGTP
                     AVSDPGAALVAAVQAAGYRALPIPGASSAVAALSVAGDATGGPFSFTGFLPARSGERA
                     LALKGLAASPNTQVLFEAPHRVEALAAALADACGARRLTVCRELTKQFETVHSLPANE
                     LPAWLEADANRSRGEFVLVLHALAVVDDDAAAPAHDAALRTLLAALPLKQAVALAAEL
                     TGAPRNALYERALALKNGADA"
     gene            1393..1788
                     /locus_tag="VNU48_08445"
     CDS             1393..1788
                     /locus_tag="VNU48_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HWI11343.1"
                     /translation="MVTRDSATTKRTTKERGDAGEARALAHLLGQGLTLVQRNYRVAH
                     GPAARGGEIDLILRERDGTLVFVEVRARQGQAHGGAAASVGAAKQRSLVFAAQHFLSG
                     QRTLPPCRFDVVAIDGERIEWLRAAFDAS"
     gene            2094..3656
                     /locus_tag="VNU48_08450"
     CDS             2094..3656
                     /locus_tag="VNU48_08450"
                     /inference="COORDINATES: protein motif:HMM:NF023425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ice-binding family protein"
                     /protein_id="HWI11344.1"
                     /translation="MNQFCRIFKSRATLIFALLPTFVAGCGGSGLDPILGTPSVAAVT
                     TLTDTTRPTVTLTVPAAGALNVATNTQVTATFSEPMDPATIKDTSFTVRNTTLGSTVS
                     GTVSYSAPARTATFTPAGAGLASNNQYTATITMAATDLAGNALAGNTAVAPNAGDDAW
                     AFGTGGSADIVPPTVVSISPADGSAGICLTKAVSATFSEPMDPATITSATFGVTAGGV
                     AVPGAVSYDAPSRVASFLPTNAAGLAASTTYLVSVKSGVNGVKDLAGNALATDRNWSF
                     ATGTQACLAPVNLRSAASFGAFGGAAGVTNQGINTVVGGNLGTTAACTLITGFHDAAN
                     VYTETPLNVGAVNGSAYCAPPAPGTVSTMAIATQARADALSAYNDLAALPPGGDPGAG
                     QLGGLVLPAGVYTAAGGTFAVTSGDLTLDAQGDTNAVWVFQSASALTVGLGATPRHVL
                     LINGAQARNVFWQVGSAARVEDGSAMVGTIIAPAGVTISTAGQTAQTTLTGRALGLTA
                     SVTMVNTTIVAP"
     gene            3653..4828
                     /locus_tag="VNU48_08455"
     CDS             3653..4828
                     /locus_tag="VNU48_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWI11345.1"
                     /translation="MKPNIDRKNKMNKLNALHLLRLAGIGSLLAAAASPASAQEAFYY
                     GGISLGQSRAKIDEARITSTLLDNGLTTTSMSRDEHDTAYKLFGGYQFNKNFGIEAGY
                     FNLGRFGFSSTTVPAGTLNGQIKLQGVNLDLVGALPLTNSLSVIGRVGAQYANARDRF
                     SGTGAVSVSNPNPSKRSANYKLGAGLQYEITPSVLVRAEAERYRINDAVGNRGDVNMF
                     SVSLVMPFGRSPTSAPRAAAPAYVEPAPAPVVAAVAPPAPAVVVPERRRVSFSADSLF
                     SFDQSDVKPEGRKALDRFAKDLDGTRFEVITVEGHTDRLGSHAYNQKLSSRRAESVKT
                     YLVSSGGVDSTKVTATGKGESASVTKPDDCKGTKPSAKLIACLQPDRRVEVEVTGTR"
     gene            4908..5504
                     /locus_tag="VNU48_08460"
     CDS             4908..5504
                     /locus_tag="VNU48_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HWI11346.1"
                     /translation="MLEQRIQQQFFDSADLKYAAAEVLAKPIADAVSALVGCITAGGK
                     VLACGNGGSAADAQHFAAEFVGRFERERPGLAAVALTTDSSILTAIGNDYDFNSIFSK
                     QVQALGAPGDVLLAISTSGNSSNVVAAIEAARAKEMVVIAMTGHKGGKIRELLTETDV
                     LICVPHDRTARIQEVHLLTLHCLCDAVDLQLLGEQENT"
     gene            5501..6130
                     /locus_tag="VNU48_08465"
     CDS             5501..6130
                     /locus_tag="VNU48_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWI11347.1"
                     /translation="MTSRTSRRLAALLATLAAAAALGGCAPLLLGGAVVGGSMMAIDR
                     RTSGAQIDDQTIEVKGIKRVNDSIGDRGSVSVTSYNHIVLLTGTVTNEADKAAVERAV
                     GAIESVRSVVNEVAIGTAASFGNRSSDTLVTSKVKASFVDAKDLQVNAFKVVTERGVV
                     YLMGRVTEREANRAADIARGVSGVAKVVKVFEIVTEAELADLQTKPAKP"
     gene            complement(6153..7046)
                     /locus_tag="VNU48_08470"
     CDS             complement(6153..7046)
                     /locus_tag="VNU48_08470"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI11348.1"
                     /translation="MSTRTYEPTPARRVAFLGLGVMGHPMAGHLAAAGHRVTVYNRTP
                     AKAAQWVAEHGHASATTPRAAADGADIVFACVGNDADLRAVVLGEQGAFAGMKPGAIF
                     VDHTTASAEVARELAAAALSRGLRFIDAPVSGGNLGAINGTLTVMCGGDAAAFAAMQP
                     VAMAFSKAVTLLGASGAGQLAKMVNQVAIAGLVQGLAEAVAFGEKAGLDMKAVLGVIG
                     QGAAQSWQMDNRGPTMIEDRFDFGFAVDWMRKDLGLVLDEAKRNGARLPVTALVDQFY
                     ADLQAQGGNRLDTSSLLRRLR"
     gene            complement(7123..>7472)
                     /locus_tag="VNU48_08475"
     CDS             complement(7123..>7472)
                     /locus_tag="VNU48_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HWI11349.1"
                     /translation="SLNLKALVSQRLLPRQEGKGRIAAVEILLNTPLISELIFKGEVS
                     EIKEIMKRSRELGMQTFDQALFDLYEGQAITLEDALRNADSVNDLRLQIKLHSHRARS
                     SDLSAGTEHLTIV"
BASE COUNT         1205 a         2653 c         2534 g         1080 t
ORIGIN      
        1 ggatcggccg gcgcatacag cgaatgcgcc gagcccacgc gactgagggc cgcgaccgcg
       61 gactgcccct ggcgaccgat cgcccgcgcg gccaccgccg cctcgttcag cgcgacgagc
      121 gaaatgtcgt cggaatacgc aaaggcctgt ttttcgccgt gcacggcgcg gatgccgacg
      181 ccgcggtcga tggaaaacga gcccgccttg acgatgcctt cctcgaggct ccagccttcg
      241 ctgcgcgtgt actggaagta caggtcggcg tcgtcgatgc ggtgcgtggc gatcgtcgcc
      301 agcgccttcg acagggtcgc gtcggtcagg ccgaagggct cgagcagcag cgcgcgcgcg
      361 acagccaggc gttcgatcgt gggttcgcgg gagatcatcg gcggcgtcct cggaggtgaa
      421 cgggcattgt aggagtcggc cccgtgtgcg gtgcgaagtc gccgccttgc ccctgcgcga
      481 actcaggcgt cggcgccgtt cttcagcgcc agcgcgcgtt cgtagagcgc gttgcgcggt
      541 gcaccggtca gttcggcggc cagcgcgaca gcctgcttca gcggcagcgc cgcgagcagc
      601 gtgcgcaggg ccgcgtcgtg tgccggtgcg gccgcgtcat cgtccacgac cgccagcgca
      661 tgcagcacca gcacgaactc gccgcggctg cggttcgcgt cggcctcgag ccaggccggc
      721 agctcgttcg ccggcaggct gtgcacggtc tcgaactgct tggtcagctc gcggcagacg
      781 gtgagccgac gcgcaccgca agcgtcggcc agcgccgcgg ccaacgcctc gacgcggtgc
      841 ggcgcctcga acagcacttg cgtgttcggg cttgccgcca accccttcag cgccagtgcg
      901 cgctcgccgc tgcgcgccgg caggaagccg gtgaagctga aggggccacc ggtcgcgtcg
      961 ccggcgacgc tgagagccgc gaccgcgctg ctggcccccg ggatcggcag cgcccgatac
     1021 ccggctgcct gaaccgctgc gaccagcgcc gcgccggggt cgctgacggc cggcgtgccg
     1081 gcatcgctga cgtaggcgac gcgctcgccg cgtgccagcc gcagcagcac gccggcggcg
     1141 gcctcgcgct cgttgtgctg gtgcagcgcg atcagcgcct tgccctcgat gccgagctgg
     1201 cgcagcagcg gcgcgctgtg gcgcgtgtcc tcgcaggcca ccgcgtcgac cagcgcgagc
     1261 acatggattg cacgcagcgt gatgtcggcg aggttgccga tcggcgtggc caccacgtac
     1321 agtgcgccgg tcgggtaatg ctgcgcgccg gccgtggcgg cggcggcttg cagcatcaac
     1381 ggggcgacgg gcgtggtcac aagggattcg gcgacaacga agagaacaac gaaagagcgc
     1441 ggcgacgcgg gcgaggcgcg cgctctggcg cacctgttgg gccagggcct gaccctggtg
     1501 cagcgcaatt atcgggtcgc gcacggcccc gcggcgcgcg gcggcgagat cgacctgatc
     1561 ctgcgcgagc gcgacggcac gctggtgttc gtcgaagtgc gcgcacgcca aggccaggcc
     1621 catggcggcg cggcggccag cgtcggcgcc gccaagcagc gcagcctggt gttcgcggcg
     1681 cagcacttcc tgagcggcca gcgaacattg ccgccctgcc gcttcgacgt ggtcgcgatc
     1741 gacggcgagc gcatcgagtg gctgcgagcc gccttcgacg cgtcctgaag ccgaaccgga
     1801 agaccggcgc cgagcgcctt cgcaggttcg atccaggcga gccgcatctc cttgtcctac
     1861 gtcggcacaa gcctttcggg ccgctggcgc gggtgcttgt cgcatccgcg atccggagcc
     1921 cgagcgcagc atcgcgactc gggcgaagcg gcaccggccg ctcctgcgga agttgaccaa
     1981 accgcctttc ggactcgaag gttcgttggt gcacagacag cggcggcgcc acccttcaca
     2041 acgcactcat tgattccaga agcttgatgc ggccatcccg cagggaagaa accatgaacc
     2101 agttctgccg catcttcaaa tctcgcgcca cgctgatctt cgccttgctg cccaccttcg
     2161 tggcgggctg cggcgggagt ggcctcgacc cgatcctggg aacgccgagc gtcgccgccg
     2221 tcacaacgct gaccgacacg acccgtccca ccgtcacctt gaccgtgccg gccgccggtg
     2281 cgctcaacgt ggccaccaac acgcaggtca ctgcgacgtt cagcgagccg atggatcccg
     2341 cgacgatcaa ggacaccagc ttcaccgtga ggaacacgac gctcgggtcg accgtatccg
     2401 ggacggtgag ctactcggcg cccgcacgga ccgccacgtt cacgccagcc ggggccggct
     2461 tggcgagcaa caaccagtac accgcgacga tcaccatggc ggccaccgac ctggccggca
     2521 acgcactggc gggcaacacc gcggtcgccc cgaacgccgg ggacgacgcc tgggccttcg
     2581 gcaccggcgg cagcgctgat atcgtgcctc cgaccgtcgt ctccatcagc ccggccgacg
     2641 ggagcgccgg catttgcctg acgaaggccg tcagcgccac tttcagcgaa ccgatggatc
     2701 ccgcgacgat cacgtccgcg acgttcggcg tgaccgctgg cggcgtcgcc gtcccaggcg
     2761 cggtgagcta tgacgcgcca agccgcgtcg caagcttcct gcccacgaat gcggccggcc
     2821 tcgcggcgag cacgacctac ctcgtcagcg tcaagtccgg ggtcaacggc gtcaaggatc
     2881 tggccggcaa tgcgctggcc accgaccgga actggagctt tgcgaccggg acgcaggcct
     2941 gcctggcacc ggtcaacctg cgctcggccg cgtccttcgg cgcgttcggc ggcgctgcgg
     3001 gcgtcacgaa ccaggggatc aacaccgtcg tcggcgggaa cctcggcacg accgcggcct
     3061 gcacgctgat caccggcttc cacgacgcgg ccaatgtcta cacggagacg ccgctcaacg
     3121 tgggggcggt gaacggcagc gcctattgcg cgccgccggc gccgggaacc gtctcgacga
     3181 tggcgatcgc gacccaggcc cgtgccgatg ccctctcggc ctacaacgac ctcgccgcgc
     3241 tgccccctgg tggcgacccg ggtgccggcc agctgggcgg gctggtgctg cctgcgggcg
     3301 tctatacggc cgccggtgga accttcgccg tcaccagcgg cgacctgacc ctggatgcgc
     3361 agggcgacac caacgccgtg tgggtcttcc agagcgcttc cgcgctgacc gtgggccttg
     3421 gcgccacgcc gcgccacgtg ctgctgatca acggcgcaca agccaggaac gtgttctggc
     3481 aagtcggcag cgcggcccgc gtcgaagacg gcagcgcgat ggtgggcacc atcatcgcgc
     3541 cggccggcgt gacgatctcg accgccggtc agaccgcaca gaccaccctg acgggacgcg
     3601 ccctcggcct cacggcctcg gtgaccatgg tcaacaccac catcgtcgcg ccatgaagcc
     3661 gaacattgat cggaagaaca agatgaacaa gctcaacgca ctgcacctgc tccgtcttgc
     3721 ggggatcggc tccctgcttg cggcggcggc ttcgcccgcc tctgcgcagg aggccttcta
     3781 ctatgggggg atctcgctcg gccagtcgcg agcgaagatc gatgaggcgc gcatcacctc
     3841 gacgcttctg gacaacgggc tgaccaccac gtcgatgtcg cgcgacgaac acgacacggc
     3901 ctacaagctt ttcggtggct accagttcaa caagaacttc ggcatcgagg cgggctactt
     3961 caaccttggc aggttcggct tttcgtcgac cacggtgccc gcgggcacat tgaacggcca
     4021 gatcaagctc cagggtgtca acctggacct ggtgggcgcg ctgccactca cgaacagcct
     4081 gtcggtgatc gggcgcgtgg gcgcccagta cgccaatgcg cgcgaccgct tcagcggcac
     4141 gggcgcggtc tccgtgtcga accccaaccc gtcgaagcgc tccgccaact acaagctcgg
     4201 cgccggcctg cagtacgaga tcaccccatc ggtcctggtg cgagctgaag ccgagcgcta
     4261 ccggatcaac gacgccgtgg gcaaccgcgg cgacgtcaac atgttctcgg tgagcctggt
     4321 gatgccgttc ggtcgttccc cgacatcggc accgcgtgca gcggctccgg cctatgtgga
     4381 gccggcgccg gcgccggtcg tcgccgcagt ggccccgccg gcacccgccg tggtcgtccc
     4441 ggagcggcgt cgcgtgagct tctcggccga ttcgctgttc agcttcgacc agtcggatgt
     4501 gaagcccgag ggccgcaagg cgctggaccg gttcgcgaag gacctcgacg gcacccgatt
     4561 cgaggtgatc acggtcgagg gtcacaccga caggctcggc tcccacgcct acaaccagaa
     4621 gctgtcctcg aggcgggccg aatcggtcaa gacctatctg gtgagctcgg gcggcgtcga
     4681 ttcgacgaag gtcacggcga ccggaaaggg cgagtcggca tcggtcacga agcccgatga
     4741 ctgcaagggc accaagccga gcgccaaact catcgcctgc ctgcagcccg atcgacgtgt
     4801 cgaagtcgag gtgaccggca cgcgctgact tgaacgacgt ggccggggct ggttgcccgc
     4861 acccgatgga accggcatgg tgcactggtg tcatatgatc catgctcatg cttgaacaac
     4921 gcatccaaca gcagttcttc gacagcgccg acctgaagta cgccgccgcc gaggtgctgg
     4981 ccaagccgat cgccgacgcg gtcagcgcgc tggtcggctg catcaccgcg ggcggcaagg
     5041 tgctggcctg cggcaacggc ggctcggccg ccgacgccca gcacttcgcg gccgagttcg
     5101 tgggccgctt cgagcgcgaa cgcccgggcc tggccgcggt ggcgctgacg accgacagct
     5161 cgatcctgac cgcgatcggc aacgactacg acttcaactc gatcttctcc aagcaggtcc
     5221 aggcgctcgg cgcgcccggc gacgtgctgc tcgcgatcag caccagcggc aactcgtcga
     5281 acgtggtcgc ggcgatcgaa gcggcacgcg cgaaagagat ggtcgtgatc gcgatgaccg
     5341 gccacaaggg cggcaagatc cgcgagctgc tgaccgaaac cgacgtcctc atctgcgtcc
     5401 cgcacgatcg caccgcgcgc atccaggagg tgcacctgct gacgctgcac tgcctgtgcg
     5461 acgcggtcga cctgcaactg ctgggcgaac aggaaaacac atgacctcaa gaacttcacg
     5521 gcgcctcgcg gcgctgctcg cgaccctggc cgccgcggcc gcactgggcg gctgcgcgcc
     5581 gctgctgctc ggcggcgccg tcgtcggcgg ctcgatgatg gccatcgacc ggcgcacctc
     5641 gggtgcgcag atcgacgacc agacgatcga ggtgaaaggc atcaagcgcg tcaacgacag
     5701 catcggcgac cgcggcagcg tcagcgtgac cagctacaac cacatcgtgc tgctgaccgg
     5761 cacggtgacg aacgaggccg acaaggcggc ggtcgagcgc gcggtcggcg cgatcgaaag
     5821 cgtgcgctcg gtcgtcaacg aggtcgcgat cggcaccgcc gcctcgttcg gcaaccgctc
     5881 gagcgacacg ctggtgacca gcaaggtcaa ggcgagcttc gtcgacgcga aggacctgca
     5941 ggtcaacgcg ttcaaggtcg tcaccgagcg cggcgtcgtc tacctgatgg gccgcgtcac
     6001 cgaacgcgag gcgaaccgcg ccgccgacat cgcgcgcggc gtcagcggcg tggcgaaggt
     6061 cgtcaaggtc ttcgagatcg tcaccgaagc cgaactcgcc gatctgcaaa ccaagccggc
     6121 caagccctga tcgaccgcgc agcgcgcagg cgtcagcgca ggcggcgcag caggctcgag
     6181 gtgtccaggc gattgccgcc ctgggcctgc aggtcggcgt agaactgatc gaccagtgcg
     6241 gtgaccggca ggcgcgcacc gttgcgtttg gcctcgtcca gcaccagccc caaatccttg
     6301 cgcatccagt cgaccgcaaa gccgaagtcg aagcggtcct cgatcatcgt cgggccgcgg
     6361 ttgtccatct gccagctctg cgccgcgccc tggccgatca cgccgagcac ggccttcatg
     6421 tccagcccgg ccttctcgcc gaaggcgacc gcctcggcca ggccttgcac gaggccggca
     6481 atcgcgacct ggttcaccat cttcgcaagc tgcccggccc cgctcgcgcc gagcagcgtc
     6541 actgccttcg agaacgccat cgcgaccggc tgcatcgcgg cgaaggccgc cgcgtcgccg
     6601 ccgcacatca ccgtcagcgt gccgttgatc gcgcccaggt tgccgcccga taccggcgcg
     6661 tcgatgaacc gcaggccgcg cgacagcgcc gcggccgcga gttcgcgtgc gacctcggcc
     6721 gaggcggtcg tgtggtcgac gaagatcgcg cccggcttca tgcccgcaaa cgcgccctgc
     6781 tcgccgagca cgacggcgcg caggtcggcg tcgttgccga cgcaggcgaa gacgatgtcg
     6841 gcaccgtcag ccgccgcgcg cggcgtggtg gcgctcgcgt ggccgtgctc ggcgacccat
     6901 tgcgcggcct tcgccggcgt gcggttgtag accgtgacgc ggtgcccggc cgcggcgagg
     6961 tgcccggcca tcggatgacc catcacgccc agaccgagga aggcgacccg acgcgccggc
     7021 gtgggttcgt aggtccgtgt actcatcgcg ctctcaatca aaacagaagg ggccggcatt
     7081 gccgaccccg ctatcgtccg tgcccgaagg ccccggcgcc gactatacga tcgtcaggtg
     7141 ctcggtgccg gccgacaggt cgctgctgcg cgcgcgatgg ctgtgcagct tgatctgcag
     7201 ccgcaggtcg ttgaccgagt cggcgttgcg caaggcgtct tcgagcgtga tcgcctggcc
     7261 ctcgtacagg tcgaacagcg cctggtcgaa ggtctgcatg cccagctcgc gcgagcgctt
     7321 catgatctcc ttgatctcgg agacctcgcc cttgaagatc agctccgaga tcagcggcgt
     7381 gttcaacagg atctcgaccg cggcgatgcg gcccttgcct tcctggcgcg gcaagaggcg
     7441 ctgcgacacc agcgccttca ggttcagcga ca
//
LOCUS       DATMJB010000389         4606 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_776365, whole genome shotgun sequence.
ACCESSION   DATMJB010000389 DATMJB010000000
VERSION     DATMJB010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776365"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VNU48_08480"
     CDS             complement(<1..276)
                     /locus_tag="VNU48_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HWI11350.1"
                     /translation="MKAISLGVIAVTVVLGGCSLAPKYSRPPSPVAPEWPQGAAYEQS
                     GAGAAHVAEIGWRQFYLDDRLRSVIALSLANNRDLRVATLNIERARAT"
     gene            complement(276..3416)
                     /locus_tag="VNU48_08485"
     CDS             complement(276..3416)
                     /locus_tag="VNU48_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014631362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWI11351.1"
                     /translation="MVNYFIDRPIFSWVIAIVIMLAGALAIPSLPIAQYPAIAPTQIT
                     VSVSYPGASAETVQSTMVQVIEQQLSGLDHLRYFTSSSNKDGSMEIALTFEQGTNADI
                     AQVQVQNKLALATPQLPPEVQQQGIRVVKGSQNYLLIVGFVSTDGSMDRQAIGDFLVS
                     SVQDPLSRTPGVGDYTVFGSQYAMRVWLDPSKLDSYALTPGDVSNALRAQNVQISSGE
                     IGGLPAVAGQQLNASVIGPTRLKTMDEFEQVLLRVNRDGSQVRLGDVARVELGSEFQG
                     VDSRYNGKPAAGIGIKLASGANALDTANAVRATLDKLRPQFPTGLEVVYPYDTTPFVR
                     LSIEEVVKTLFEAIVLVFLVMFLFLQNFRATLIPTLAVPVVLLGTFAVLAASGYTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLSPKEATRKSMGQISGALVGVAMVLS
                     AVFLPMAFFGGSAGIIYRQFSITIVSAMTLSVIVALVFTPSLCATLLKPIQKGHHEQK
                     RGFFGWFNRVFDAGAHRYASGVQHLVGRTGRYILVYAVIVAAVGLLFMRIPAGFLPDE
                     DLGVIAVEVRTPPGAAIVRTNEAMAQVRKYLLNDEKESVSSVYEVAGFSFSGRGQNTG
                     VLYINLKDWDERRDEQHHAQAIAQRTMAHFAGYKDAMVFAFPPPAIIELGNATGFDAQ
                     LQDRAGKGHAALMEARNQLLGAAAHNPALVAVRPNGRDDEPQYRIDIDWEKASAFGLS
                     VADVNNTLSTAWGSSYVNQFIDRGRVKRVYVQGDAPYRMRPEDFANWYVRNASGTTVP
                     FSAFATGHWTNGPPKLDRYNGTSSAEILGQPAPGYSTGQAMQEVERLVDQLPPGFGIE
                     WTGLSYEERQSGGQAPMLYALSIAVVFLSLAALYESWSIPFSVLLVVPLGVLGTVLGA
                     LLRGLNNDVYFQVALLTTVGLAAKNAILIVEFAKENVEHGMGLIESAVHAARQRLRPI
                     LMTSLAFILGVMPLALSSGAGSGSQNAVGTGIVTGTLAATVLGIFFIPLFFVAVRSLL
                     TRRQPVLLTPSTSEAQ"
     gene            complement(3418..>4606)
                     /locus_tag="VNU48_08490"
     CDS             complement(3418..>4606)
                     /locus_tag="VNU48_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWI11352.1"
                     /translation="TRRTLIMNVRTIALISCAGLLAVSASVGCTSRTAAAPAPPPTAE
                     VGVVQVATQPVTMTAELPGRTVPVTVSEVRPRIRGIIQKRLFAEGQTVKAGQVLYEID
                     PALYQADYANAQATLARAEASRFSAKLLAERYAEVVKVEAVSKQESDNAQAAYRQADA
                     EVAAARAALDTARITLDYTHITAPIAGVVSASVYTPGALVTTDQLNPLTTVRQLDPIY
                     VDITQSSAQLLDLQRQLASGRLRRADGTIKVQLRLEDGTTYEHEGTLAFTDAAVDETT
                     GMVHLRTVFPNPKRVLLPGKYVRAVLPLGVDEQALLVPQQALTHDARGNATVLLVNGD
                     SKVEQRVVKAERTVRDQWLVTEGLAAGDRVVVEGVQKVQPGASVRVAEGAATVARVEA
                     GGR"
BASE COUNT          781 a         1511 c         1514 g          800 t
ORIGIN      
        1 cgtcgcgcgt gcgcgctcga tgttgagcgt cgccacgcgc aggtcgcgat tgttcgccag
       61 gctcagcgca atcacgctgc gcaggcgatc gtcgagatag aactgccgcc atccgatctc
      121 cgcgacgtgc gcagcaccgg caccggactg ctcataggcg gctccttgtg gccattcggg
      181 ggcgacgggc gacggtggcc gcgagtattt cggcgcaagg ctgcatccgc cgagcaccac
      241 ggtgacggcg atcacgccaa gagagatggc tttcattact gggcctccga ggtcgacggt
      301 gtgagcagga caggctggcg tcgcgtcaag agactccgca ctgccacgaa gaacaagggg
      361 atgaagaaga tgccgagcac cgtcgctgcg agcgtgccgg tgacgatccc ggtgccgacc
      421 gcgttctggc tgcccgaccc ggcgccgctc gagagcgcga gcggcatgac gccaaggatg
      481 aatgccagcg acgtcatcag gatcggccgc aggcgctgac gcgcggcgtg caccgctgat
      541 tcgatcaggc ccatgccgtg ttcgacattc tccttggcaa attccacgat gaggatcgcg
      601 ttcttcgccg cgagaccgac ggtcgtcagc agcgcaacct ggaagtacac gtcgttgttc
      661 agtccgcgga gcagagcacc caggaccgtg cccagcactc cgagcggtac gacgagcagc
      721 accgagaacg ggatcgacca gctctcgtag agcgcggcca gcgagaggaa cacgaccgcg
      781 atcgacagcg catacagcat cggcgcctgt ccgcccgact gccgttcctc atacgagagg
      841 ccggtccatt cgatgccgaa cccgggcggc agctggtcca ccaggcgctc gacttcctgc
      901 atggcctggc ccgtgctgta gccgggggcg ggctggccga ggatttcggc tgacgaggtg
      961 ccgttgtagc gatccagctt cggcgggccg ttcgtccagt gtcccgtcgc aaaggcggag
     1021 aacggcaccg tggtgcccga cgcgttccga acgtaccagt ttgcgaagtc ctcggggcgc
     1081 atgcgatacg gcgcgtcgcc ctggacgtag acacgtttga cgcggccgcg atcgatgaac
     1141 tgattcacgt aggacgaacc ccacgccgtc gacaacgtgt tgttcacatc ggcgaccgac
     1201 aggccgaagg cgctggcctt ttcccagtcg atgtctatcc ggtattgcgg ctcgtcgtcg
     1261 cggccgttcg gacgcaccgc aaccagcgcc gggttgtgcg ccgctgcacc caggagctgg
     1321 ttgcgggctt ccatcaatgc ggcgtgcccc ttacccgccc tgtcctgcag ctgggcgtcg
     1381 aagccggtgg cattacccag ctcgatgatc gccggcggtg gaaacgcgaa gaccatcgcg
     1441 tccttgtagc ccgcgaagtg cgccatcgtc cgctgcgcga ttgcctgggc gtgatgctgc
     1501 tcgtcgcggc gttcgtccca gtccttcaaa ttgatgtaca ggactccggt gttctgaccg
     1561 cgtccgctga agctgaatcc cgcgacctcg tataccgagg acacgctttc cttctcgtcg
     1621 ttgagcaggt acttgcgcac ctgcgccatc gcctcgttcg tgcggacgat ggcagcgcca
     1681 ggcggtgtcc tgacttcgac cgcgatgacc cccaggtcct cgtcggggag aaagccggcc
     1741 gggatacgca tgaagagcag accgacagcc gcgacgatca cggcgtagac gagtatgtag
     1801 cggccggtgc gaccgaccag gtgctgcacg cccgacgcgt agcgatgcgc accggcatcg
     1861 aacacccggt tgaaccagcc gaaaaagccg cgcttctgtt cgtggtgccc cttctggatc
     1921 ggcttcagca gcgtggcgca gagcgacggc gtgaacacga gcgccacgat gacggacagt
     1981 gtcatcgccg agacgatcgt gatcgagaac tgccggtaga tgatgccggc cgagccgccg
     2041 aagaacgcca tcggcagaaa tactgccgac agcaccatcg cgacgccaac gagcgcccct
     2101 gagatctgcc ccatcgactt gcgcgtggcc tccttcggcg acagcccttc ttcggccatt
     2161 acgcgctcca cgttttccac gaccacgatc gcgtcgtcga ccagcaggcc gatcgcgagc
     2221 accatcccga acatcgtgag ggtgttgatc gtgtatccgc tcgcggcgag cacggcgaag
     2281 gtcccgagca acacgaccgg caccgcgagg gtgggaatga gtgtggccct gaagttctgc
     2341 aggaacagga acatgaccag gaagacgagc acgatcgctt cgaacagcgt cttgacgact
     2401 tcctcgatcg acagccgcac gaatggcgtc gtgtcgtacg gatagacgac ctccagaccg
     2461 gtcgggaact ggggacgcag cttgtcgagc gtggcccgca cggcatttgc tgtgtcgagg
     2521 gcgttggctc cggacgccag cttgatcccg atgccggccg caggcttgcc gttgtagcgg
     2581 gaatcgacgc cctggaattc gctcccgagc tcgacgcgag cgacgtcacc caggcgcacc
     2641 tgcgatccgt cgcggttgac gcgcagcagc acctgctcga actcgtccat cgtcttgagg
     2701 cgcgtcggcc cgatcacgct cgcattgagc tgctggcccg ctactgcggg caacccgccg
     2761 atttcacccg acgagatctg cacgttctgg gcgcggagcg cgttgctgac atcgcccggc
     2821 gtcagcgcgt agctgtcgag tttcgacgga tcgagccaga cgcgcatcgc gtactgcgag
     2881 ccaaacaccg tgtaatcgcc gacacctggc gtgcggctca agggatcctg cacgctggac
     2941 acgaggaaat cgccgatggc ctggcggtcc atgcttccgt ctgtcgagac gaagccgacg
     3001 atcaggaggt agttctggct gcccttcacg acccggatgc cctgttgctg gacttcgggc
     3061 ggcagctgcg gcgtggcaag cgcgagcttg ttctgcacct gcacctgcgc gatgtccgcg
     3121 ttggtgccct gctcaaacgt cagggcgatc tccatgctgc cgtccttgtt gctggacgac
     3181 gtgaaatagc gcaggtgatc gaggccgctc agctgctgct cgatgacctg caccatggtg
     3241 ctttgcacgg tctcagcgga ggcaccagga taggagacgc tgacggtaat ctgcgtcggc
     3301 gcgatggccg ggtattgcgc gatcggcagc gacgggattg cgagcgcgcc cgccagcatg
     3361 atgacgatcg cgatcaccca ggagaagatc ggacggtcga tgaagtagtt gaccatgtca
     3421 gcgcccgcct gcttctaccc gcgccaccgt cgcggcccct tcggccacgc ggacgctggc
     3481 gccgggctgc accttctgaa cgccctccac gacaacgcga tcgccggcgg cgagcccttc
     3541 ggtgaccagc cattgatcgc gaacggtccg ctcggccttg acgacgcgct gttcgacctt
     3601 gctgtcgccg ttgaccagga gcaccgtcgc gttcccgcgc gcgtcatgcg tgagggcctg
     3661 ctgcggcacg agcagcgcct gctcgtcgac gccgagcggc aagaccgcgc ggacgtactt
     3721 gccaggcagc agcacgcgtt tcggattggg gaacaccgtt cgcaaatgga ccatgccggt
     3781 ggtttcgtcg accgccgcgt cggtgaaagc gagcgtgccc tcgtgttcgt aggtcgtgcc
     3841 gtcctcgagg cgcagctgca ccttgatcgt tccgtcggcg cgacgcagcc ggccgcttgc
     3901 gagctgccgc tggaggtcca gtaactgcgc gctcgactgc gtgatgtcga cgtagatcgg
     3961 atccagctgg cgcacggtcg tcagcgggtt cagctggtct gtcgtcacga gcgcgcccgg
     4021 cgtgtagacc gacgccgaca ccacgccggc gattggcgcc gtgatatgcg tgtaatccag
     4081 ggtgatgcgt gcggtgtcga gcgcggcgcg tgcggcagcg acctcggcgt cagcctgacg
     4141 atacgcggcc tgtgcgttgt cgctctcctg cttcgatacc gcttcgactt tcaccacttc
     4201 ggcgtaccgc tcggcgagga gcttcgctga gaaccgcgac gcttcggccc gtgcgagcgt
     4261 cgcctgggcg ttggcgtagt cggcctggta caacgccggg tcgatctcat acagcacctg
     4321 gccggccttg acggtctgtc cctcggcgaa gagccgtttc tggatgatgc cgcgaatccg
     4381 ggggcgtact tcggagacgg tgaccggcac cgtccggccg ggcaactctg cggtcatcgt
     4441 caccggctgc gttgccacct gcaccacgcc gacttccgcc gtcggcggcg gcgcgggcgc
     4501 cgccgccgtt cgcgaagtgc agccgacgct tgccgacaca gcaagcaagc cggcgcagga
     4561 aatgagcgcg atcgtgcgga cgttcataat gagcgttctc cgtgtt
//
LOCUS       DATMJB010000390         3845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_559500, whole genome shotgun sequence.
ACCESSION   DATMJB010000390 DATMJB010000000
VERSION     DATMJB010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559500"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1128
                     /locus_tag="VNU48_08495"
     CDS             <1..1128
                     /locus_tag="VNU48_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWI11353.1"
                     /translation="MALGAFLAGVLLAESEYRRELETDIEPFKGLLLGLFFIAVGMSI
                     DFAVVLRSPTLIAGVVLGFLMLKAAVLWAMGRAMPCPKPERPVFIILLAQGGEFGFVV
                     FQTAAGAGVIDAQVSSLLVAAVAISMLVTPLLLVAADKWWIPLLAGQGRKTTLEEIRD
                     PQNQSVIIAGFGRYGQVVGRMLYANGVKPTVLDVDAEQIDAMRKFGWPVFYGDATRLD
                     LLRTAGAAKAKVFVLAIDDVEQSVAVAQMVRENFPELTIVARARNVRHYFELHDLGVT
                     LIERETLDSALMSARSVLEQLGWERHQARTLALRFRRHNVDQLVKMGPHRKDEAKLIA
                     VAKQGRQQLEELFAQERDEAAKRQARIGWNDDADTRGTPPA"
     gene            1226..1630
                     /locus_tag="VNU48_08500"
     CDS             1226..1630
                     /locus_tag="VNU48_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HWI11354.1"
                     /translation="MERNTRQREAVLGAIEHAGRALTPGEILELAQGTVARLNLSTIY
                     RQLHALQDEARIVKVLLPGQAARFEAACHERQRHAAHHHHHFHCEACDRVYALHACPG
                     SMQELAPAGFVVSAHEITLRGLCAECSRGGVR"
     gene            1627..2319
                     /locus_tag="VNU48_08505"
     CDS             1627..2319
                     /locus_tag="VNU48_08505"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11355.1"
                     /translation="MTGVELPASGMALFFLLGLRHGIEPDHIAAIDGLTLRAHDHRER
                     HAPWTGSLFALGHGLAIAAIAVGVSLLAASVSLPATLVQVIDWLPIVLLTLLGAWNLR
                     ALLAPGAYRPSSVRMRLIPAGLRERTDLWSTVAIGLLFALVIDTVAHVSAWSVFATHR
                     GGWWAGVVAGLLFSLGMLIASTADSQLISRVLRSAGSDAAASRLRRGIGWFVVVLSFG
                     VVLQAVVAKLGA"
     gene            complement(2331..2906)
                     /locus_tag="VNU48_08510"
     CDS             complement(2331..2906)
                     /locus_tag="VNU48_08510"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HWI11356.1"
                     /translation="MNITTEQLFDNARTHNGFAPEPIPQATLHQLYELVKWGPTSANC
                     SPARFIFITTPEAKAKLLPAMSPGNHDKTKAAPLTVIIGMDMAFYEKLPQLFPHADAK
                     AWFVGNQPLIDATAFRNSSLQGGYFILAARALGLDCGPMSGFDAAKVDAAFWAGTAVK
                     TNFIVNLGHGDASKLFGRSPRLSFDEACQIV"
     gene            3026..3457
                     /locus_tag="VNU48_08515"
     CDS             3026..3457
                     /locus_tag="VNU48_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HWI11357.1"
                     /translation="MFAPSQHDVRRFFCDTRRKQRQALPLTPMEAIAADWIAAHPEYF
                     EALDDVAAALAAVYEVEGGRSNPFLHLSMHLTISEQIAIDQPRGIKQAFALLATRLGS
                     AHEAQHEVMECLGEMVWASQRSGLPPDGERYLECVRRRATR"
     gene            complement(3461..>3845)
                     /locus_tag="VNU48_08520"
     CDS             complement(3461..>3845)
                     /locus_tag="VNU48_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWI11358.1"
                     /translation="EDQYAIRALRAGAFGYVNKGGDPQLIVQAVRTVSQGRKYVTPEI
                     AQMLVESLTTPMIANAHEKLSDRELQTLVMIASGKRLSDIAEELMLSPKTVSVYRARV
                     LEKLGLANNSEMTVYAIRNGLVGSE"
BASE COUNT          547 a         1304 c         1377 g          617 t
ORIGIN      
        1 atggcgctcg gcgcgttcct cgccggcgtg ctgctcgccg agagcgaata ccggcgcgaa
       61 ctcgaaaccg acatcgagcc gttcaagggc ctgctgctcg gcctgttctt catcgccgtg
      121 ggcatgagca tcgacttcgc ggtggtgctg cgctcgccga cgctgatcgc cggcgtggtg
      181 ctcggcttcc tgatgctgaa ggcggcggtg ctgtgggcga tggggcgtgc gatgccgtgc
      241 cccaagcccg agcggccggt tttcatcatc ctgctcgcgc agggcggcga gttcggcttc
      301 gtcgtgttcc agaccgccgc gggcgccggc gtgatcgatg cgcaggtctc gtcgctgctg
      361 gtcgccgcgg tcgcgatctc gatgctggtc acgccgctgc tgctggtggc cgcggacaag
      421 tggtggatcc cgctgctcgc cggccagggc aggaagacca cgctggagga gattcgcgac
      481 ccgcagaacc agtcggtgat catcgccggc ttcggccgct acggccaggt cgtgggccgc
      541 atgctctacg ccaacggcgt caagccgacc gtgctcgacg tcgacgccga acagatcgac
      601 gcgatgcgca agttcggctg gccggtgttc tacggcgacg ccacgcggct ggacctgctg
      661 cgcaccgccg gcgcggccaa ggcaaaggtg ttcgtgctcg cgatcgacga cgtggaacag
      721 agcgtcgcgg tcgcgcagat ggtgcgcgag aacttccccg agctgacgat cgtcgcgcgc
      781 gcccgcaacg tgcgccacta cttcgagctg cacgacctgg gcgtgacgct gatcgaacgc
      841 gagacgctgg actcggcgct gatgagcgcg cgcagcgtgc tcgagcagct cggctgggag
      901 cgccaccagg cgcgcacgct ggcgctgcgc ttccggcgcc acaacgtcga tcagctcgtc
      961 aagatggggc cgcaccgcaa ggacgaagcc aagctgatcg cggtcgcgaa acagggccgg
     1021 cagcagctcg aggagctgtt cgcgcaggag cgcgacgagg ccgccaagcg ccaggcacgc
     1081 atcggctgga acgacgacgc ggacacgcgc ggcacgccgc cggcctgatg gccggccagg
     1141 cggcgtgccg ggaccggcgc gcgcggcgca aggccttcgc ggccacgcgc tctgttgcca
     1201 atcgatttgc aataagatcg cggccatgga acgcaacacc cggcaacgcg aggccgtgct
     1261 cggcgcgatc gagcacgccg ggcgcgcgtt gacgccgggc gagatcctcg aactcgcgca
     1321 gggcacggtc gcgcgcctga acctgtcgac gatctaccgc cagctgcacg cgctgcagga
     1381 cgaggcacgc atcgtcaagg tgctgctgcc cggccaggcg gcgcgcttcg aggccgcgtg
     1441 tcacgaacgc cagcgccacg ccgcccacca ccatcatcac tttcactgcg aagcctgcga
     1501 ccgcgtgtac gcgctgcatg cctgcccggg gtcgatgcag gagctggcgc cggcgggctt
     1561 cgtcgtcagc gcgcacgaga tcacgctgcg cgggctgtgc gccgagtgca gccggggcgg
     1621 cgtgcgatga ccggcgtcga actgccggcg agcggcatgg cgctgttctt tctgctcggc
     1681 ctgcgccacg gcatcgaacc cgaccacatc gccgcgatcg atggcctcac cctgcgcgcg
     1741 cacgaccacc gcgaacgcca cgcgccgtgg accggcagcc tgttcgcgct cggtcacggc
     1801 ctggcgatcg cggcgatcgc ggtcggcgtg agcctgctgg cggcctcggt cagcctgccg
     1861 gcgacgctgg tgcaggtcat cgactggctg ccgatcgtgc tgctgaccct gctcggcgcg
     1921 tggaacctgc gcgcgctgct cgcgcccggc gcctaccggc cgagctcggt gcgcatgagg
     1981 ctgatcccgg ccggcttgcg cgagcgcacc gacctgtggt cgacggtggc gatcgggctc
     2041 ttgttcgcgc tcgtgatcga cacggtcgcg cacgtctcgg cctggagcgt gttcgccacg
     2101 caccgcggcg gctggtgggc cggcgtggtc gcgggcctgc tgttctcgct cggcatgctg
     2161 atcgccagca ccgccgattc gcagctgatc tcgcgcgtgc tgcgcagcgc cggcagcgac
     2221 gccgcggcct cgaggctgcg ccgcggcatc ggctggttcg tcgtcgtgct gtcgttcggc
     2281 gtcgtgctgc aggcggtggt cgccaagctc ggcgcctgaa ggcgcgcccg tcagacgatc
     2341 tggcaggcct cgtcgaagct caggcgcggg ctgcggccga acagcttgct cgcgtcgcca
     2401 tgcccgaggt tgacgatgaa gttggtcttc accgcggtgc cggcccagaa cgccgcatcg
     2461 accttggccg cgtcgaagcc gctcatcggc ccgcaatcga ggccgagcgc gcgcgccgcg
     2521 aggatgaagt agccgccctg gaggctcgag ttgcggaacg ccgtggcgtc gatcagcggc
     2581 tggttgccga cgaaccaggc cttcgcatcg gcgtgcggaa acagctgcgg cagcttctcg
     2641 tagaacgcca tgtccatgcc gatgatcacc gtcagcggcg cggccttggt cttgtcgtgg
     2701 ttgcccggcg acatcgccgg cagcagcttc gccttggctt cgggcgtcgt gatgaagatg
     2761 aagcgtgccg ggctgcagtt ggccgaggtc gggccccact tcaccagctc gtagagctgg
     2821 tgcagcgtgg cctgcggaat cggctcgggc gcgaaaccgt tgtgggtgcg ggcgttgtcg
     2881 aagagctgtt cggtggtgat gttcatggcg tcgccggcgt cggcagtggt tggacgatga
     2941 ggcgattctg cgcagcgggc gcgagccggg tccgaaaaga ttcgtcgacc ccgttcggct
     3001 gcgttgaagg atgcagaatc cctcgatgtt cgccccttcg cagcacgacg ttcgccgctt
     3061 cttctgcgac acccgccgca agcagcggca ggcgctgccg ctgacgccga tggaggcgat
     3121 cgcggccgac tggatcgccg cgcacccgga atacttcgag gcgctcgacg atgtggccgc
     3181 ggcgctggcg gccgtctacg aagtcgaggg cgggcgcagc aacccgttcc tgcatttgtc
     3241 gatgcacctg acgatcagcg agcagatcgc gatcgaccag ccgcgcggca tcaagcaggc
     3301 gttcgcgctg ctcgcgaccc ggctgggctc ggcacacgag gcgcagcacg aggtgatgga
     3361 atgcctgggc gagatggtct gggcctcgca gcgcagcggg ctgccgccgg acggcgagcg
     3421 ctacctggag tgcgtgcggc ggcgcgcgac gcggtgaagc ctactcgctg ccgaccaacc
     3481 cgttgcggat cgcgtagacc gtcatctcgg agttgttcgc caggccgagc ttctcgagca
     3541 cgcgggcgcg gtagacgctg acggtcttcg ggctgagcat cagctcctcg gcgatgtccg
     3601 acaggcgctt gcccgaggcg atcatcacca gggtctgcaa ctcgcggtcc gagagcttct
     3661 catgcgcgtt ggcgatcatc ggcgtggtca ggctctcgac cagcatctgc gcgatctccg
     3721 gcgtcacgta tttgcgcccc tgcgacaccg tgcgcaccgc ctgcacgatg agctgcgggt
     3781 cgccaccctt gttgacgtag ccgaaggcgc cggcgcgcaa cgcgcgaatc gcgtactggt
     3841 cttcc
//
LOCUS       DATMJB010000391         3061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_826003, whole genome shotgun sequence.
ACCESSION   DATMJB010000391 DATMJB010000000
VERSION     DATMJB010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826003"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="VNU48_08525"
     CDS             complement(<1..1584)
                     /locus_tag="VNU48_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWI11359.1"
                     /translation="MFGAACVGALAQTSLLPVTVTATRFPAASADLPFGVSVLTADAI
                     RASGATTVNEAIMKLLGVPGRIDPYGGGDYGLDLRGFGATADNNQVVVVDGIRINEAD
                     LGGTRLAGIPIDAVERIEVIRGSAAVLYGEGATGGAIVITTKGGRGGARQNQADVYAA
                     AGSFGLREARASATLATGGFALDATANQRDTDNDRDNYRSRTDGASLAGQWSNDWLRA
                     GARYAYDHLDTGLPGALSAAQYAADPHQTSTPLDSAGIRNNRRGLFGEATLGDWQIGV
                     DAGWRTKTLTSDYISSGFVYAYDVDASTYALRARHAAKFGAYANSFIAGIDQGEWTRT
                     VPGAFGSEANQRSRAVYLKDELALPGGMRLSAGWRTARVKEDNSSATGSLDDHPNAWE
                     LGIVQPVGGGVSVYGRYGRSFRLANVDEFGFTTPGVALKPQTSRDAELGLRWTHARGS
                     AELRAYRSALTNEIGYDPNAIGPSSAFGFNGANVNFDPTRREGLELEVTQALAANANV
                     RVNAATRRARFSAGAYQGKD"
     regulatory      complement(1737..1976)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2031..2264
                     /locus_tag="VNU48_08530"
     CDS             2031..2264
                     /locus_tag="VNU48_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HWI11360.1"
                     /translation="MSKIEELAERVERLLVRHAELRRTNALLAQQVSVVTHERDNLRS
                     RLNAARSRIDALLERLPADSPAAPDDDATRAAP"
     gene            2261..2575
                     /locus_tag="VNU48_08535"
     CDS             2261..2575
                     /locus_tag="VNU48_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HWI11361.1"
                     /translation="MKQMEVTILGQSYLLGCPDGGEVALLEAVGSVDREMSAIRDGGK
                     VKARERIAVLAALNLAYALAGRPAARVPPSAAATAASGEVDIDQLIERLDQALSADGQ
                     LL"
     gene            2820..>3061
                     /locus_tag="VNU48_08540"
     CDS             2820..>3061
                     /locus_tag="VNU48_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HWI11362.1"
                     /translation="MRHWLMKSEPSECSIDDLAAAPKQTVPWVGVRNYQARNFMRDEM
                     RPGDGVLFYHSSCPEPGVAGIAEVASGPYPDASQFDP"
BASE COUNT          439 a         1053 c         1067 g          502 t
ORIGIN      
        1 gtccttgccc tggtaggcgc cggcgctgaa acgggcgcgc cgcgtcgcgg cgttgacgcg
       61 cacgttggcg ttggccgcga gcgcttgcgt gacttcgagc tcgaggcctt cgcggcgcgt
      121 cggatcgaag ttgacgttgg caccgttgaa gccgaacgcg ctgctcgggc cgatggcgtt
      181 cgggtcgtag ccgatctcgt tcgtcagcgc gctgcggtac gcgcgcagtt cggcgcttcc
      241 ccgcgcgtgc gtccagcgca ggccgagttc ggcatcgcgc gaggtctgcg gcttcagcgc
      301 gacaccgggg gtcgtgaagc cgaactcgtc gacgttggcg aggcggaagc tgcgaccgta
      361 gcggccgtag accgaaacgc caccgcccac cggctggacg atgccgagtt cccaggcatt
      421 cgggtgatcg tccagggacc cggtcgcgct gctgttgtcc tccttgacgc gcgcggtgcg
      481 ccagccggca ctcaggcgca tgccgccagg cagcgcgagc tcgtccttca ggtacaccgc
      541 ccgcgagcgc tggttcgcct cggagccgaa cgcgcccggc acggtgcgcg tccactcgcc
      601 ctggtcgatg ccggcgatga agctgttggc gtaagcgccg aatttcgccg catggcgcgc
      661 ccgcagcgcg taggtgctgg cgtcgacgtc gtacgcatag acgaagccgc tgctgatgta
      721 gtcgctggtc aatgtcttgg tgcgccagcc ggcgtccacg ccgatctgcc agtcgcccag
      781 cgtggcctcg ccgaacagcc cgcggcggtt gttgcggatg ccggccgaat ccagtggcgt
      841 gctcgtctgg tgcgggtcgg ccgcgtactg cgccgcactc agcgcaccgg gcaggccggt
      901 gtcgaggtgg tcgtaggcat agcgcgcgcc ggcgcgcagc cagtcgttgc tccactggcc
      961 ggcaagtgac gcgccgtcgg tgcgcgagcg gtagttgtcg cggtcgttgt cggtgtcgcg
     1021 ctggttcgcg gtcgcgtcga gcgcgaagcc gccggtggcc agcgtcgcgc tggcgcgcgc
     1081 ctcgcgcagc ccgaagctgc ccgccgcggc gtagacatcg gcctgattct ggcgtgcgcc
     1141 gccccggccg cccttggtgg tgatgacgat cgcgccgccg gtggcgccct cgccgtagag
     1201 caccgccgcg ctgccgcgga tgacctcgat gcgctcgacc gcgtcgatcg ggatgccggc
     1261 cagccgcgtg ccgccgagat ccgcctcgtt gatgcggatg ccgtcgacga cgacgacctg
     1321 gttgttgtcg gccgtcgcgc cgaaaccgcg cagatccagg ccgtagtcgc cgccaccgta
     1381 ggggtcgatg cgaccgggca caccgagcag cttcatgatc gcctcgttca cggtggtcgc
     1441 gccggacgcc ctgatggcgt cggccgtcag cacgctgacg ccgaagggca ggtcggcgct
     1501 cgccgccggg aagcgggtcg cggtgacggt caccggcaac aggctggtct gcgccagggc
     1561 gccgacgcat gccgcgccga acatggccgc ggccaggcgc ggccgggcga aacgggcacg
     1621 cgtgccgcga ggtgctggat gcaccgaaca ggttttggac attcgaaaga ctcgcaaaga
     1681 cgttgtcgcg cacgccagct tccccgcggg cgtgcacctc ctggcgcgct gctcgcgcag
     1741 cgcaccgcct cgttggccgg tatccgggct ggcggagcaa cgcgtgcgtt ccttcccaaa
     1801 ggcggtcgcc ttcagtggtg tgtgcgcatt cgccgaagcc cgagggcttc gtccgccgta
     1861 ccgttgcggg ggcagcgcag attcggtcgc cacgcatgaa gcgtgacttc cttctgcttc
     1921 ccgtttaact gtgccggctg aacgccggca cgggcaccaa cagcgcggat tgtaaacagg
     1981 gccgaccttt agaatgcggg caacaagctc tccagcccga acaccacttc atgtcgaaga
     2041 tcgaagaact cgccgagcgg gtcgagcgcc tgctagttcg gcatgccgag ctgcggcgca
     2101 cgaacgcgct gctcgcgcag caggtgagcg tggtgacgca cgagcgcgac aacctgcgct
     2161 cgcgtctgaa cgccgcgcgc agccgcatcg atgccttgct cgagcggctg cccgccgact
     2221 cgccggccgc gcccgacgac gacgccaccc gggccgcgcc atgaagcaga tggaagtgac
     2281 gatcctcggc cagagctacc tgctcggttg ccccgatggc ggcgaggtcg cgctgctcga
     2341 agcggtcggc agcgtcgacc gcgagatgag cgcgatccgc gatggcggca aggtcaaggc
     2401 gcgcgaacgc atcgcggtgc tcgcggcgct gaacctcgcc tacgcgctgg ccgggcggcc
     2461 tgcggcgcgc gtaccgccat ctgcggcggc cacggcagcg tcgggcgagg tcgacatcga
     2521 tcaactgatc gagcgcctcg accaggcgct gagcgcggac gggcaattgc tctgaggttc
     2581 acaccttcgt cacgcgcagg cttagaatga attcgtccgt cgcattcgcg tgagttttat
     2641 atttccttga acctatgctc tttagagcaa gggcttggaa catcgcccgg ctggtgtgat
     2701 cgtctcgcgt cagatgaacc cgaagccggg ctgacctcgc ccacctgaac tcctggttca
     2761 ggaatgcggc tcatggttcg cggcggacac ctctcttccc cttcgatcct cccgtcccca
     2821 tgcgccactg gctgatgaag agcgagccct ccgagtgctc gatcgacgac ctcgcggccg
     2881 cgccgaaaca gaccgttccg tgggtcggcg tgcgcaacta ccaggcgcgc aatttcatgc
     2941 gcgacgagat gcgccccggc gacggcgtgc tcttctatca ctcgtcctgc ccggagcccg
     3001 gtgtcgccgg catcgcggaa gtcgcgagcg ggccttatcc cgacgcgagc cagttcgatc
     3061 c
//
LOCUS       DATMJB010000392         3157 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_529189, whole genome shotgun sequence.
ACCESSION   DATMJB010000392 DATMJB010000000
VERSION     DATMJB010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529189"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1235)
                     /gene="glyS"
                     /locus_tag="VNU48_08545"
     CDS             complement(<1..1235)
                     /gene="glyS"
                     /locus_tag="VNU48_08545"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HWI11363.1"
                     /translation="MTTTAKNLLVELFVEELPPKALRKLGDVFASGLAEALKAQGLAS
                     DASVTTPFASPRRLAAHITAVRAQAADKAVSHKLMPVSVGLDAAGAATPALLKKLAGL
                     GADASAVPQLERRPDGKTEALFFDSTVTGATLAEGLQKALLEALAKLPIPKVMQYQLA
                     DGWTSVHFVRPAHGLVALHGEAVVPVTALGLAAGRTTHGHRFEAALDPIELMHADDYA
                     ERLESQGAVIASFDARRKEIARQLRSAAATQGLTPIDDAALLDEVTALVERPNVLVCR
                     FEEAFLAVPPECLILTMKANQKYFPLLDASSEAAASGREGRLTNRFLIVSNIRPLDAS
                     RVIEGNERVVRPRLADAKFFFDQDRRRTLESRVAGLDKVVYHGKLGTQGERTARVRAI
                     ARAIGARLGGPELAAQADRA"
     gene            complement(1232..2158)
                     /gene="glyQ"
                     /locus_tag="VNU48_08550"
     CDS             complement(1232..2158)
                     /gene="glyQ"
                     /locus_tag="VNU48_08550"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HWI11364.1"
                     /translation="MLTFQQIILELQDYWGAQGCALLQPYDMEVGAGTSHTATFLRAI
                     GPEPWKAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPAPANILELYLGSLEALGF
                     DLKANDIRFVEDDWENPTLGCWGLGWEVWLNGMEVTQFTYFQQVGGIDCKPITGEITY
                     GLERLAMYLQGVDNVYNLTWTDGLSYGDVYKQNEVEQSTYNFEHSDVEFLLTAFAAHE
                     KQSKHLMEQRLALPAYEQLLKAGHSFNLLDARGAISVTERAAYIGRIRNLARAVAKSY
                     KESRRRLVPPFPMAPRAWAEEVGAQMDRDAAA"
     gene            complement(2188..>3157)
                     /gene="lnt"
                     /locus_tag="VNU48_08555"
     CDS             complement(2188..>3157)
                     /gene="lnt"
                     /locus_tag="VNU48_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HWI11365.1"
                     /translation="PLAATVAVLGAAALLGPAQFSRPSGSLTVTLLQGNVAQDEKFAV
                     ELMPRSLEWTLAQLLAARGALVVAPETVIPLLPDQLDAGYWEAITDHFQRGAQAVLLG
                     LPLGDERVGYTNSAAGISAATAALPGGFYRYDKHHLVPFGEFVPTGFHWFTEMMNIPL
                     GDFNRGPVGPPSFGFRGERIGANICYEDLFGEELAARFVDAAQAPTMFANISNIGWFG
                     DTSAIAQHLEISRMRTLEFQRPMLRATNTGATAVIAHTGRVSAALPRFTQGVLDAQVQ
                     GRTGLTPFAWWAARFGLSPLWLLGAAIVAWRVLGARRASAGAAPAL"
BASE COUNT          451 a         1093 c         1115 g          498 t
ORIGIN      
        1 gcgcggtccg cctgcgcggc gagctcgggc ccgcccaggc gcgcgccgat cgccctggcg
       61 atcgcgcgca cgcgcgccgt gcgttcgccc tgggtgccga gcttgccgtg gtagaccacc
      121 ttgtcgaggc cggcgacgcg cgattcgagc gtgcgcctgc ggtcctggtc gaagaagaac
      181 ttcgcatccg ccaggcgcgg ccgcacaacg cgctcgttgc cttcgatcac gcggctcgca
      241 tcgagcggac ggatgttgct gacgatcagg aagcggttcg tcaggcgccc ctcgcggccg
      301 ctggcggccg cttccgatga agcgtcgagc agcgggaagt acttctggtt cgccttcatc
      361 gtcaggatca ggcattcggg cggcaccgcg aggaacgctt cctcgaagcg gcagaccagc
      421 acgttcggcc gctcgacgag cgcggtgacc tcgtcgagca gcgccgcgtc gtcgatcggc
      481 gtcaggccct gcgtcgccgc ggccgagcgc aactggcgcg cgatctcttt gcggcgcgcg
      541 tcgaagctgg cgatcaccgc gccctgcgat tcgagccgct cggcgtagtc gtcggcgtgc
      601 atcagctcga tcggatcgag cgcggcctcg aagcggtggc cgtgtgtggt acggccggcg
      661 gcgaggccga gcgccgtcac cggcacgacc gcctcgccat gcagcgcgac caggccgtgc
      721 gccgggcgca cgaagtgcac gctggtccag ccatcggcga gctggtactg catcaccttc
      781 gggatcggca gcttcgcgag cgcctcaagc agggccttct gcaggccttc ggccagcgtc
      841 gcgccggtca cggtgctgtc gaagaagagc gcctcggtct tgccgtccgg ccggcgctcg
      901 agctgcggca cggccgacgc gtcggcgccg aggccggcga gcttcttcag cagcgccggg
      961 gtcgcggcac ccgctgcatc gagcccgaca ctcacgggca tcagcttgtg cgacaccgcc
     1021 ttgtcggccg cctgcgcacg aaccgcggtg atgtgggcgg cgagccggcg cggcgacgcg
     1081 aacggcgtcg tgaccgacgc gtcgctcgcc aggccctgcg ccttcaacgc ctcggccagg
     1141 ccgctcgcga agacgtcgcc caatttcctc agcgccttcg gcggcagctc ttcgacgaac
     1201 agttcgacga gcaggttctt cgcggtcgtc gtcatgccgc cgcgtcccga tccatctgcg
     1261 ccccgacctc ttcagcccag gcgcgcggcg ccatcgggaa cggcgggacg aggcgtcgtc
     1321 tgctctcctt gtagctcttg gcgaccgccc gcgcgaggtt gcggatgcgg ccgatgtacg
     1381 ccgcgcgctc ggtcacgctg atcgcgccgc gcgcatccag caggttgaag ctgtggccgg
     1441 ccttgagcag ctgttcgtac gcgggcaacg caagccgctg ctccatcagg tgcttcgact
     1501 gcttctcgtg cgcggcgaac gcagtgagca ggaactcgac atcactgtgc tcgaagttgt
     1561 aggtgctctg ctcgacctcg ttctgcttgt agacgtcgcc gtagctcaga ccgtcggtcc
     1621 aggtcaggtt gtagacgttg tcgacgccct gcaggtacat cgccaggcgc tccagcccgt
     1681 aggtgatctc gccggtgatc ggcttgcagt cgatgccgcc gacctgctgg aagtaggtga
     1741 actgcgtcac ctccatgccg ttgagccaga cttcccagcc caggccccag cagccgagcg
     1801 tggggttctc ccagtcgtcc tcgacgaagc ggatgtcgtt ggccttcagg tcgaagccga
     1861 gcgcctcgag cgagccgagg tagagctcga ggatgttcgc cggcgcgggc ttgagcacga
     1921 cctggtactg gtagtagtgc tgcagccggt tcgggttctc gccgtagcgg ccgtccttcg
     1981 gccggcggct gggctgcaca tacgcggcct tccacggctc ggggccgatc gcgcgcaaga
     2041 aggtcgcggt gtggctggtg ccggcgccga cttccatgtc gtagggctgc agcagtgcgc
     2101 agccttgcgc gccccagtag tcctgcaact cgagaatgat ctgctgaaaa gtgagcatcg
     2161 gcactgaggc tgcgtaaacg ctcgattcta gagggctggc gccgcgccgg cgctcgcgcg
     2221 ccgggcgccg agcacgcgcc acgcgacgat cgccgcgccc agcaaccaca gtggcgacag
     2281 gccgaagcgc gcggcccacc acgcgaacgg cgtcagcccg gtgcggccct gcacctgggc
     2341 gtcgagcacg ccctgcgtga agcgcggcag cgcggcgctg acgcggccgg tgtgggctat
     2401 cacggcggtc gcgccggtgt tggtggcccg cagcatcggg cgctggaatt cgagcgtgcg
     2461 catgcgcgag atctccaggt gctgggcgat cgcgctcgtg tcgccgaacc agccgatgtt
     2521 gctgatgttg gcgaacatcg tcggggcctg cgcggcatcg acgaagcgcg ccgcgagctc
     2581 ctcgccgaac aggtcttcgt agcagatgtt cgcgccgatg cgctcgccgc ggaacccgaa
     2641 actcggcggc ccgacgggcc cgcggttgaa gtcgccgagc gggatgttca tcatctcggt
     2701 gaaccagtga aagccggtcg ggacgaattc gccgaagggc acgaggtggt gcttgtcgta
     2761 gcggtagaag cccccgggca gcgcggcggt cgcggccgag atgccggcgg ccgagttcgt
     2821 gtagcccacg cgctcgtcgc ccagcggcaa accgagcagc acagcctgcg caccgcgctg
     2881 gaagtggtcg gtgatcgctt cccagtagcc cgcgtcgagc tggtcgggca gcaacgggat
     2941 caccgtttcg ggcgcgacca cgagcgcgcc gcgcgcagcc agcagttgcg cgagcgtcca
     3001 ttcgagcgag cgcggcatca gctcgaccgc gaacttctcg tcctgcgcga cattgccctg
     3061 cagcagcgtg accgtcaggc tgccgctcgg ccgcgagaat tgcgccgggc cgagcagcgc
     3121 cgccgcgccg agcaccgcga ccgtcgcggc cagcggc
//
LOCUS       DATMJB010000393         2844 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_771713, whole genome shotgun sequence.
ACCESSION   DATMJB010000393 DATMJB010000000
VERSION     DATMJB010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771713"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VNU48_08560"
     CDS             complement(<1..417)
                     /locus_tag="VNU48_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI11366.1"
                     /translation="MTSGLIRIRGARQHNLKNLDLDIRTGEMTVVTGPSGSGKSSLVF
                     DTLYAEGQRRYVETFSAYARQFLDRMDRPAVDRVDGVPPAIAIDQTNPVRTSRSTVGT
                     MTEINDHLKLLFARTGKLFCRQCARAVRRDTPDSIHA"
     gene            555..2654
                     /locus_tag="VNU48_08565"
     CDS             555..2654
                     /locus_tag="VNU48_08565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI11367.1"
                     /translation="MTDDPPASTPAESPASSGVLNAAARNDRRLFDSLNEGVWERDLK
                     TGEVWYSPRYKSLLGFEDHELPNLVDAVRERLHPDDLAGARAAFARAAQTPGQGGEGL
                     ARLRVKDGSYRWFRGRFTVWPDAQGQPGVLVGALHDVQDQIAATEALKAQQAVLEQRV
                     RERTQGLEAALQQAEAQRLAAERANRTKASFLAHMSHELRTPLNGVLGMTQLAQALAL
                     GAEQRRYLELAQQSGQALLGFLDDVLDFAQAEAGRLQLREEAIDLAALAAETLRSFMP
                     ALRRKGLQVGFDYVGDITRVRADAGRVRQIIANLLGNAIKYTEAGGILLVVAVEADAQ
                     AGHCRVRLRVRDSGVGMDEATVARVFEPFEQGDSGIDRRHGGSGLGLSVVQMLARLMG
                     GEVSVRSRPGRGSEFRIELRLAAEPDQPLRTRAAAATAGGHAWVLVRDPIHGDGICQR
                     LARIGWSAEVLPDVAAAILRLRAAPAGGTAPECVLIGEDALGDDTDLAQLRHGLRPGV
                     PVTLLLHPDFDLGTVHAATERLQLRIAIAPLAPADVHALVQRDAAPQPVADLAQMPAP
                     RGAKDRQASVLIVEDNPMNQIIAREMVAALGLRPAVVCSGEEAMLSCRSTPPDLVLMD
                     IQMPGMDGLETTRRLRALQAAGELRRFPIIALTAHATASDRAASLAAGMDEHLTKPVQ
                     LEQLRRVLWQWLAAEQG"
BASE COUNT          407 a         1014 c         1049 g          374 t
ORIGIN      
        1 ggcgtggatc gagtcgggcg tgtcgcgccg caccgctcgc gcgcactgcc ggcagaagag
       61 cttcccggtg cgggcgaaga ggagcttcag gtggtcgttg atctcggtca tcgtgccgac
      121 cgtcgagcgc gaggtgcgca ccgggttggt ctggtcgatg gcgatcgcgg gcggcacgcc
      181 gtcgacgcgg tcgaccgccg gccggtccat gcggtcgagg aactggcgcg cgtaggcgct
      241 gaaggtctcg acgtagcggc gctgcccttc ggcatacagc gtgtcgaaca ccaggctcga
      301 cttgcccgag ccgctcggcc cggtgaccac cgtcatctcg ccggtgcgga tgtccagatc
      361 caggttcttg aggttgtgct gacgggcacc gcgaatgcgg atcagaccgg aagtcatgcg
      421 ggaactactt tccagagcga gccgagcatt ctatggatcg cagttgttcg gcgcgcgcat
      481 catcaaaaga gcccatgtcc gggcaccccc cccaaagcta ccatgcccga tcagtccgaa
      541 tcgcccaccg ccgcatgacc gacgatccgc ccgcgtccac ccccgccgaa agccccgcat
      601 cgtcaggcgt gctcaatgcc gccgcccgca acgaccggcg cctcttcgac agcctgaacg
      661 aaggcgtctg ggagcgcgac ctgaagaccg gcgaggtctg gtactcgccg cgctacaaga
      721 gcttgctggg cttcgaggac cacgaactgc cgaacctcgt cgacgccgtg cgcgagcgcc
      781 tgcatcccga cgacctggcc ggcgcgcgcg ccgcgttcgc acgggcggcg cagacgccgg
      841 gccagggcgg cgaggggctg gcacggctgc gcgtcaagga cggcagctac cgctggttcc
      901 gcggccgctt caccgtctgg cccgacgcgc agggccagcc cggcgtgctg gtcggcgcgc
      961 tccacgacgt gcaggaccag atcgccgcca ccgaggcgct gaaggcgcag caggcggtgc
     1021 tcgagcagcg cgtgcgcgag cgcacccagg gcctggaggc ggcgctgcag caggccgagg
     1081 cccagcgcct cgcggccgag cgcgcgaacc gcaccaaggc cagctttctc gcccacatga
     1141 gccacgaact gcgcacgccg ctgaacggcg tgctcggcat gacgcagctc gcgcaggcac
     1201 tggcgctggg cgccgagcag cggcgctacc tcgaactggc gcagcagtcg ggccaggcgc
     1261 tgctgggctt tctcgacgac gtgctcgact tcgcgcaggc cgaggccggg cgcctgcagc
     1321 tgcgcgagga ggccatcgac ctcgccgcgc tcgcggccga aacgctgcgc agcttcatgc
     1381 ccgcgctgcg gcgcaagggc ctgcaggtcg gcttcgacta cgtcggcgac atcacgcggg
     1441 tgcgcgccga tgccgggcgc gtgcggcaga tcatcgccaa cctgctcggc aacgcgatca
     1501 agtacaccga ggccggaggc atcctgctgg tcgtcgcggt cgaggccgac gcccaggccg
     1561 ggcactgtcg cgtgcggctg cgggtgcgcg acagcggcgt cggcatggac gaggccaccg
     1621 tcgcacgcgt gttcgagccc ttcgaacagg gcgactccgg catcgaccgg cgccacggcg
     1681 gcagcggcct cgggctgtcg gtggtgcaga tgctcgcgcg cctgatgggc ggcgaggtgt
     1741 cggtgcgcag ccggcccggg cgcggctccg agttccgcat cgagctgcgg ctcgcggccg
     1801 agcccgatca gccgctgcgc acgcgcgccg ccgcggcgac cgccggcggc cacgcgtggg
     1861 tgctggtgcg cgacccgatc cacggcgacg gcatctgcca gcgcctcgcg cgcatcggct
     1921 ggtcggccga ggtgctgccc gacgtggccg cggcgatcct gcggctgcgc gccgcgccgg
     1981 ccgggggcac tgcacccgag tgcgtgctga ttggcgaaga cgcgctcggc gacgacaccg
     2041 acctggcgca gctgcgccac ggcctgcggc ccggcgtgcc ggtgaccctg ctgctgcacc
     2101 ccgacttcga cctcggcacc gtgcatgccg ccaccgagcg gctgcagctg cgcatcgcga
     2161 tcgcgccgct ggcgccggcc gacgtccatg cgctcgtcca gcgcgacgcg gcgccgcagc
     2221 cagttgccga cctcgcgcag atgccggcgc cgcgcggggc gaaggatcga caggccagcg
     2281 tgttgatcgt cgaggacaac ccgatgaacc agatcatcgc gcgcgagatg gtggccgcgc
     2341 tgggtctgcg gccggccgtg gtctgcagcg gcgaggaagc gatgctgagc tgccgcagca
     2401 cgccgcccga cctggtgctg atggacatcc agatgcccgg catggacggg ctggagacga
     2461 cgcgccggtt gcgcgcgctg caggctgcgg gcgagctgcg gcgcttcccg atcatcgcgc
     2521 tgaccgccca cgcgacggcc tcggaccgcg ccgccagcct ggccgcgggc atggacgagc
     2581 acctgacgaa gccggtgcag ctcgagcagc tgcggcgcgt gctgtggcag tggctggcgg
     2641 cggagcaagg ttaagggcgg gccgtcaggc ttcgacggac gtgggcgtgg gtcgcgggcc
     2701 gctcacggcc agcagcggcc gattacgcga gcggccgcgc ctgccccgaa acggtcatgg
     2761 cgaccggctt gcgattgaag attccgatga ccggcgcgcc atgctcgcgc aggaacggct
     2821 cgatcttgcg gaacagcgcg tggt
//
LOCUS       DATMJB010000394         3051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_495635, whole genome shotgun sequence.
ACCESSION   DATMJB010000394 DATMJB010000000
VERSION     DATMJB010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495635"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..436
                     /locus_tag="VNU48_08570"
                     /pseudo
     CDS             1..436
                     /locus_tag="VNU48_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520988.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(430..2100)
                     /locus_tag="VNU48_08575"
                     /pseudo
     CDS             complement(430..2100)
                     /locus_tag="VNU48_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466364.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            complement(2111..>3051)
                     /locus_tag="VNU48_08580"
     CDS             complement(2111..>3051)
                     /locus_tag="VNU48_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWI11368.1"
                     /translation="AWQEASVGTEANGLRLASVRVNVGDVVKRGQVLATFAPDTMLSD
                     VAQVRASVAEAEATLADAAANAERARGLQATGALSEAAINQYLTAERTAKARLDAQRA
                     MAQMQSLRLKQTQVLAPDNGIISARSATVGAVLPAGQELFRMIRQGRLEWRAEVAAND
                     MAQLKPGMAALVTAGGAPAIKGTVRMVAPTVDPQTRNGIVYVDLPPAPGAARAGMFAR
                     GEFDVGSGSGLTLPQSAVLLRDGFSYVLRVGPDSKLTQLKVGVGRRLGDRIEITSGVE
                     PSTRVVAAGGGFLGDGDTVRVVDAVAARPLPAAIVK"
BASE COUNT          469 a         1076 c         1035 g          471 t
ORIGIN      
        1 cgccttcatg accggcatcg tcggcaagtt cttcgtgcag ttcggctgga cggcggcgat
       61 cgcggtgctg ttctcgctgg tggtcgcgcg catgctgacg ccgatgatgg cggcctacct
      121 gctgaagccg cccaagaacg cgcatgccga agatccgaag tggctcgcga tctacatgaa
      181 gtgggcggcc tggtgcctga agcaccgcat caagacgacg ctcgcggcga tcgtcttcct
      241 ggtcggctcg ctgatgctgg tgccgctgct gccgaccggc ttcatcccgc cggacgacct
      301 gtcgcagacg caggtgacct tgacgatgcc gcccggcagc aagctggccg acacctacgc
      361 cgcggccgaa catgcacgcg agatcgtgca gaagaacaag cacgtgctgc tggtctacac
      421 cgcgatcggc ggcggcagcg agaggttgac ctgcgtctgc gacaggtcgt cgggcgggat
      481 gaagccggtc ggcagcagcg gcaccagcat gaacgagccg acgaggaaca cggtcgccgc
      541 gatcgtcgtc aggatgcggt gcttcagcgc cgccttgacc cagcgcatgt agatcgtgat
      601 ccagcggcct tccttgtgct ccttcgtcgg caccttcagg atgtaggccg acatcatcgg
      661 cgtcagcatg cgtgcgacca ccagcgagat gaagaccgcg atcgccgccg tccagccgaa
      721 ctgcacgaag aacttgcccg gcacaccgct catgaacgcg gtcggcagga acaccgcgat
      781 cagcgtgaac gtggtcgcga tcaccgccag gccgatctcg tcggccgctt ccatcgccgc
      841 atcgaacggg ctcttgccca tgcgcaggtg gcgcatgatg ttctcgatct cgacgatcgc
      901 gtcgtcgacc aggatgccga cgacgagcga cagcgacagc aaggtcacgc cgttcagcgt
      961 gaagccgaac aggtacatca caccgaacgc cgggatgatc gacagcggca gcgcggtcgc
     1021 cgagaccagc gtggcgcgcc agtcgcgcag gaacagccac acgaccagca ccgcgagcgc
     1081 cgcgccctcg aacagcagga tcatcgagcc gtcgtagttt tcctgcaccg ggtcgacgaa
     1141 gttgaacgcc tcggtgacgg tgatctcggg gtgcgccgcc ttcaaggtgg cgagctgctt
     1201 gcgcacgccg gccgcgacct cggtctcgcc ggcgccacgc gtgcgcacga tctcgaaacc
     1261 gaccaccggc ttgccgttga gcagcgcggc cgagcgcttc tcggcggtgg tgtcgctcac
     1321 tgtcgcgacc tggtcgaggc gaatgcgccg gccgtcgccg agcgtgactt ccatgcgcgc
     1381 caactcttcg gccgacttga ccgtcgcgat cgtgcgcacc gattgctcgg cgccgcccac
     1441 gtcggtgcgc ccaccggacg cttcctgctg cgtcatgcgc agctggcgcg agatgtcggc
     1501 ggcggtcgcg ttcagggcct gcagccgggc cggatcgagt tcgacgcgca cctcgcgcgt
     1561 cacgccgccg acgcgcgcga ccgagccgac accgcgcacg ctgagcatct gcttggtgac
     1621 ggtgttgtcg acgaaccagg agagcgcttc gtcgtccatg cgggtcgacg caaccgtgta
     1681 ggtcaggatc ggcgaacccg ccagctccat cttcgtgacg accggatcgc gcagatcgcc
     1741 gggcaggtcg gcccttacgc gcgagacggc ggcgcgcacg tcgtcgacgg cttcctgggt
     1801 cggcttctcg aggcggaact cggtcgtcac gatcgcgctg ccgtcctgca cggtcgtgta
     1861 gatgtgcttg acgccctgca gcgtcgcgat cgagttctcg atcttgcgtg cgacctcggt
     1921 ttcgagctgc gccggcgacg cgccgggcag cgacgcggtc acggtgacgg tgggcaggtc
     1981 gatgtccgga aagttctgga tcttcatcgc cttgaaggcc agcaggccca tcaaggtcag
     2041 catgatgaac agcagcaccc ccgggatcgg gttgcggatc gaccaggccg atacattcat
     2101 cgcgaaccct tcacttgacg atcgccgcgg gcagcggccg cgccgccacc gcgtcgacca
     2161 cgcgcaccgt gtcgccgtcg ccgaggaagc cgccgccggc cgcgacgacg cgggtgctcg
     2221 gctcgacgcc gctcgtgatc tcgatccggt cgcccaggcg ccggccgacg ccgaccttga
     2281 gctgcgtcag cttcgaatcg ggcccgacgc gcagcacata gctgaagccg tcgcgcagca
     2341 acaccgcgct ctgcggcagc gtcagccccg acccgctgcc gacatcgaac tcgccgcgcg
     2401 cgaacatgcc ggcgcgcgcc gcgcccggcg cgggcggcag gtcgacgtag acgatgccat
     2461 tgcgcgtctg cgggtcgacg gtcggcgcga ccatgcgcac cgtgcccttg atcgccgggg
     2521 cgccgccggc cgtgaccagc gccgccatgc cgggcttcaa ctgcgccatg tcgttggcgg
     2581 cgacctcggc gcgccactcg agccggccct ggcgaatcat gcggaacagc tcctgccccg
     2641 ccggcagcac cgcgccgacg gtcgcgctgc gcgccgagat gatgccgttg tcgggtgcga
     2701 gcacctgggt ctgcttcagg cgcagcgact gcatctgcgc catcgcgcgc tgcgcgtcca
     2761 ggcgcgcctt cgcggtgcgt tcggccgtca ggtactggtt gatcgcggcc tcgctgagcg
     2821 cgccggtggc ctgcaggccg cgtgcgcgct cggcattggc ggcggcgtcg gccagcgtcg
     2881 cctcggcctc ggcgaccgac gcgcggacct gcgccacgtc ggaaagcatc gtgtcgggcg
     2941 cgaaggtcgc gagcacctgg ccgcgcttga cgacatcgcc gacattgacc cgcaccgacg
     3001 cgagccgcag cccgttggcc tcggtgccga cgctggcctc ttgccacgcg g
//
LOCUS       DATMJB010000395         2064 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_368346, whole genome shotgun sequence.
ACCESSION   DATMJB010000395 DATMJB010000000
VERSION     DATMJB010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368346"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VNU48_08585"
     CDS             <1..391
                     /locus_tag="VNU48_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWI11369.1"
                     /translation="GGHITGCAQVLKRLWPKLQVFAVEPSASPVISGGKPSPHPIQGI
                     GAGFIPKNLHTALLDGVIQVEAEAAREMARRAAREEGMLVGISSGATLAAIAQKLPSL
                     AAGATVLGFNYDSGERYLSIEGFLPAE"
     gene            565..1137
                     /locus_tag="VNU48_08590"
     CDS             565..1137
                     /locus_tag="VNU48_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWI11370.1"
                     /translation="MLGLSGCGYNNFQTLDEQVKAAWSEVLNQYQRRSDLIPNIVATV
                     KGEASFEQDTLTKVIEARAKATSIQATPELVNNPEAFDKFQKAQGELTGALSRLMVVA
                     EQYPQLKANQAFQDLRVTLEGTENRITVARNRYIKTVADYNVLARSFPSNLTAMVFGY
                     KVKPSFTVQNEAQISVPPSVNFDKPASAAK"
     gene            1163..>2064
                     /locus_tag="VNU48_08595"
     CDS             1163..>2064
                     /locus_tag="VNU48_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HWI11371.1"
                     /translation="MLRRLFALCVAAALGLAGAAAFAQDVLPVPPLAGRVVDQTGTLS
                     AAQAQALSAKLAAIEARRGAQVVVLIVPTTQPEDIASYGQRVADTWKVGRRDVGDGLV
                     IVVAKNDRRINIEVAKTLEGAIPDVLAGRIINEQIKPAFRANDYAGGLNAAIDRLDKA
                     IGGEALPAPTGKGGAGSAHRGLFGGFDLQDLAIFLFVGVPIAGAILTGLFGRKLGSLL
                     TGGAVGGIAWFLTASLLVAGGAGLIALFLIGVMGMGSGRIGRSGGPVIWGGGGGGGGF
                     GGGGGGGGGFSSGGGGDFGGGGASG"
BASE COUNT          310 a          701 c          754 g          299 t
ORIGIN      
        1 gggcggccac atcaccggct gtgcgcaggt gctcaaacgc ctgtggccga agctgcaggt
       61 gttcgcggtc gagccgagcg cgtcgccggt gatcagcggc ggcaagccga gcccgcaccc
      121 gatccagggc atcggcgcgg ggttcatccc gaagaacctg cacaccgcgc tgctcgacgg
      181 cgtgatccag gtcgaggccg aagccgcgcg cgagatggcg cggcgcgcgg cgcgcgagga
      241 gggcatgctg gtgggcatct cgtcgggcgc gacactcgcg gcgatcgccc agaagctgcc
      301 gtcgctggcc gccggtgcca cggtgctcgg cttcaactac gacagcggcg agcgctacct
      361 gtcgatcgaa gggttcctgc cggccgagtg atcgccgagc gtcgtggcgg tgcgccccgc
      421 cattgaaagc acgcgggccg gccgcacctc gggtgcgcgg ggtaggattg ccgcgccatc
      481 tttcggagga acaacatgac tcgtgcgatc tccctgcccg cctcgttcgg gcgcgccctc
      541 gcggccggtt tcgcggtgct tgcgatgctc ggcctgtcgg gctgcggcta caacaacttc
      601 cagaccctgg acgagcaggt caaggccgca tggtccgagg tgctgaacca gtaccagcgc
      661 cgttccgacc tgatcccgaa catcgtcgcc acggtcaagg gcgaggccag cttcgagcag
      721 gacacgctga ccaaggtgat cgaggcgcgc gccaaggcca ccagcatcca ggccacgccc
      781 gagctcgtca acaaccccga ggccttcgac aagttccaga aggcgcaggg cgaactgacc
      841 ggcgcgctga gccgcctgat ggtggtggcc gagcagtacc cgcagctgaa ggcgaaccag
      901 gctttccagg acctgcgcgt cacgctcgaa ggcaccgaga accgcatcac cgtggcgcgc
      961 aaccgctaca tcaagacggt cgcggactac aacgtgctgg cgcgcagctt cccgagcaac
     1021 ctgaccgcga tggtgttcgg ctacaaggtc aagcccagtt tcacggtgca gaacgaggcc
     1081 cagatctcgg tgccgccgag cgtcaacttc gacaagccgg cctccgcggc gaagtgatcg
     1141 acgaccggcc atcgagccgc tgatgctgcg ccgcctgttc gcgctgtgcg tcgccgcggc
     1201 gctggggctc gccggtgcgg cggccttcgc gcaggacgtg ctgccggtgc cgccgctggc
     1261 ggggcgggtc gtcgaccaga ccggcacgct gagcgccgcg caggcccagg ccctgagcgc
     1321 taaactcgcg gcgatcgagg cccggcgcgg cgcgcaggtc gtcgtgctga tcgtaccgac
     1381 gacgcagccg gaagacatcg ccagctacgg ccagcgtgtg gccgacacct ggaaggtcgg
     1441 ccgccgcgat gtcggcgacg gcctcgtgat cgtcgtcgcg aagaacgacc ggcgcatcaa
     1501 catcgaggtc gccaagacgc tcgaaggcgc gatccccgac gtgctcgccg ggcgcatcat
     1561 caacgaacag atcaagcccg cgttcagggc caacgactac gccggcggct tgaacgccgc
     1621 gatcgaccgg ctcgacaagg cgatcggcgg cgaggccttg ccggcgccga ccggcaaggg
     1681 cggcgccggc agcgcgcatc gcggcctgtt cggtggcttc gaccttcaag atctggcgat
     1741 cttcctgttc gtcggcgtgc cgatcgccgg cgcgatcctg accggcctct tcggccgcaa
     1801 gctcggctcg ctgctgaccg gcggcgcggt cggcggcatc gcctggttcc tgaccgcgag
     1861 cctgctggtg gccggtgggg cggggctgat cgcgctgttc ctgatcggcg tgatgggcat
     1921 gggcagcggg cgcatcgggc gcagcggcgg ccccgtcatc tggggcggtg gtggtggtgg
     1981 tggcggcttc ggcggcggtg gtggtggcgg cggtggtttc agctccggcg gcggtggcga
     2041 tttcggcggc ggcggcgcgt cggg
//
LOCUS       DATMJB010000396         3818 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_63623, whole genome shotgun sequence.
ACCESSION   DATMJB010000396 DATMJB010000000
VERSION     DATMJB010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63623"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1993)
                     /locus_tag="VNU48_08600"
     CDS             complement(<1..1993)
                     /locus_tag="VNU48_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWI11372.1"
                     /translation="MTKPQRWAWLISTVAATGAGLVLAFLLSIATNNPALYERHYVWL
                     FWVNVTVAALLVLVIGIAGVRLLMRVKRGKFGSRLLLKLAAIFALVGVVPGVLIYTVS
                     YQFVSRSIESWFDVKVETALDAGLKLGKGTLDAIVADVAVKTRLAAERLGETPGSAQS
                     LALERVREQLSAQDIAIVGPTGQTVLSTSGSAASLMPERPALSLLRQARNQRVVSELV
                     GLDEDPSTSGGASSARVRAIAFIPTTTFALNEEERYLLVNQLLPRTLATNALAVQAAY
                     REYQQRALARGGLRKMYIGTLTLALVLAVFGALLLAVTLSNQLARPLLLLAEGMRQVA
                     GGDLSAKPVFASRDELGGLTRSFADMTTQLSEARELVQRSVVQVEGARANLQTILDNL
                     TAGVIVFDRHGNIDTVNPGATRIMRLPLSAYRGQRLADVPGLEVFAQAVGQRFELHST
                     SPEAGERDHWQDSFELQPAASAGRERGSERASITLLVRGASMPQGATLMVFDDITELV
                     SAQRSEAWTEVARRLAHEIKNPLTPIQLSAERLQHKLGPKLEGADQAMLGRSVDTIVN
                     QVQAMKALVNEFRDYARLPAAHLIPIDLNALVAEVLGLYGAAQEAGHLHADLAPALPA
                     IVGDSTQLRQVIHNLVQNALDAVADQPDGRVRVCTEIARS"
     gene            complement(2010..2618)
                     /locus_tag="VNU48_08605"
     CDS             complement(2010..2618)
                     /locus_tag="VNU48_08605"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HWI11373.1"
                     /translation="MHCAAPHDGDSRPGAQRPGRRAVIALCLTLMLVLFAGVPAARAA
                     EPELTSFELVHNDDGLFLSYAVNLELSRSVDDALSKAVPLFFVAEAEVFRNRFYWRDQ
                     RVGYAVRRWRIVFQPLTSTYRVTFDGGLSQNYASRAEAFAAISRSARWKIAEAPQIDE
                     GSRHYVEFSFRLDTSQLPRPMQIGIGGQADWALSVQRTQKIN"
     gene            complement(2633..>3818)
                     /gene="rsmB"
                     /locus_tag="VNU48_08610"
     CDS             complement(2633..>3818)
                     /gene="rsmB"
                     /locus_tag="VNU48_08610"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HWI11374.1"
                     /translation="AVDALLLTALALLWPQAEAPYAEHTLVDQAVDAARQRAKPSAAF
                     VNAVLRRFLRERDALVAAALRDPVARFNHPRWWIARLKADWPEHWQAILDADNQHPPM
                     TLRVNARRGDAPSYVARLLAQGIAATALGEQAVALAQPCPVTQLPGFAQGEVSVQDAA
                     AQRAAPLLIGAGEDRQPLDAFGVVPQGELRPLGAARRGLRPGARVLDACAAPGGKTAH
                     LLELADLDVLALDSDPLRLTRVHETLARLGLTARSVAADAGDPAAWWDGRPFDAILLD
                     APCSAAGIVRRHPDVRWLRRPGDIAALAAAQARLLDALWPLLAPGGRLLYCSCSVFKA
                     EGRDQIDAFLQRQPGAALATSPPSPGHLLPLPDNRAERPVPENRAAADGFFYALLEKT
                     TT"
BASE COUNT          535 a         1346 c         1413 g          524 t
ORIGIN      
        1 cgctgcgggc gatctcggtg cacacgcgca cgcggccgtc cggctggtcg gcgaccgcgt
       61 cgagcgcgtt ctgcaccagg ttgtggatca cctggcgcag ctgcgtgctg tcgccgacga
      121 tcgccggcag cgccggcgcc aggtcggcgt gcaggtggcc ggcctcctgc gccgcgccgt
      181 acaggcccag cacctcggcg acgagcgcgt tcaggtcgat cgggatcagg tgcgcggccg
      241 gcaggcgcgc gtagtcgcgg aactcgttga ccagcgcctt catcgcctgc acctggttga
      301 cgatggtgtc gaccgagcgg ccgagcatgg cctgatcggc gccctcgagt ttcggaccga
      361 gcttgtgctg cagccgctcg gccgacagct ggatcggcgt cagcgggttc ttgatctcgt
      421 gcgccaggcg ccgcgccacc tcggtccagg cttcgctgcg ctgcgccgag acgagctcgg
      481 tgatgtcgtc gaagaccatc agcgtcgcgc cctgcggcat cgaggcgccg cgcaccagca
      541 gggtgatcga cgctctttcg gacccacgct cgcggccggc agacgccgcc ggctgcagct
      601 cgaacgagtc ctgccagtgg tcgcgctcgc ccgcctcggg gctggtgctg tgcagctcga
      661 agcgctgacc cacggcctgc gcgaacacct ccagccccgg cacgtcggcc aggcgctggc
      721 cgcggtaggc cgacagcggc agccgcatga tgcgcgtcgc gccggggttc acggtgtcga
      781 tgttgccgtg gcggtcgaac acgatcacgc cggcggtcag gttgtccagg atggtctgca
      841 ggttcgcgcg cgcgccctcg acctgcacga cgctgcgctg caccagctcg cgcgcctcgg
      901 agagctgcgt ggtcatgtcg gcgaacgagc gcgtcaggcc gccgagctcg tcgcgcgacg
      961 cgaacaccgg cttggccgag aggtcaccgc cggccacctg gcgcatgccc tcggccagca
     1021 gcagcagcgg ccgcgcgagc tggttcgaca gcgtgaccgc gagcagcagc gcgccgaaca
     1081 ccgccagcac cagcgccagc gtcagcgtgc cgatgtacat cttgcgcagg ccgccgcgcg
     1141 ccagcgcgcg ctgctggtat tcgcggtagg ccgcctgcac cgccagcgcg ttggtcgcga
     1201 gcgtgcgtgg cagcagctgg ttgacgagca ggtagcgttc ctcctcgttg agcgcgaagg
     1261 tcgtggtcgg gatgaacgcg atcgcgcgca cccgcgcgct gctcgcgccg ccgctcgtcg
     1321 acgggtcttc gtcgagcccg accagttcgc tgacgacgcg ctggttgcgc gcctggcgca
     1381 gcagcgacag cgcgggccgt tccggcatca ggctggcggc gctgccgctg gtgctgagca
     1441 cggtctggcc ggtcgggccg acgatcgcga tgtcctgcgc cgagagctgc tcgcgcaccc
     1501 gttcgagcgc cagcgactgg gccgaacccg gcgtctcgcc caggcgctcg gcggccagcc
     1561 gggtcttgac cgcgacatcg gcgacgatcg cgtcgagcgt gcccttgccg agcttcaggc
     1621 cggcgtcgag cgcggtctcg accttgacgt cgaaccagct ctcgatgctg cgcgagacga
     1681 actggtagct cacggtgtag atcagcacgc ccggcacgac gccgacgagc gcgaagatcg
     1741 ccgccagctt gagcagcagc cggctgccga acttgccgcg cttgacgcgc atcagcaggc
     1801 gcacgccggc gatgccgatc accagcacca gcagcgcggc gaccgtgacg ttgacccaga
     1861 acagccagac gtagtgacgc tcgtacagcg ccgggttgtt ggtcgcgatc gacagcagga
     1921 acgcgagcac cagcccggcg ccggtggcgg cgacggtgga aatgagccac gcccagcgct
     1981 gcggtttggt catgacgcga tcagaaggat cagttgatct tctgcgtccg ctgcaccgac
     2041 aacgcccagt cggcctgccc gccgatgccg atctgcatcg ggcgcggcag ctgcgaggtg
     2101 tccaggcgga agctgaactc gacgtaatgg cggctgcctt cgtcgatctg cggcgcttcg
     2161 gcgatcttcc agcgcgcgct gcggctgatc gcggcgaagg cctcggcgcg gctcgcgtag
     2221 ttctggctca ggccgccgtc gaaggtcacc cggtaggtcg aggtcaaggg ctggaacacg
     2281 atgcgccagc gccgcaccgc atagccgacg cgctggtcgc gccagtagaa ccggttgcga
     2341 aagacctcgg cctcggcgac gaaaaaaagc ggcaccgcct tgctcaaggc gtcgtcgacg
     2401 ctgcgcgaga gctcgaggtt gacggcgtag ctgaggaaca ggccgtcgtc gttgtggacc
     2461 agctcgaagc tcgtcagctc gggctccgcg gctcgcgcgg ccggcacgcc ggcgaacagc
     2521 accagcatca gcgtcaagca cagcgcgatc accgcccggc gacccgggcg ctgcgcgccg
     2581 ggccgggaat cgccgtcgtg gggtgccgcg cagtgcatgg gggccgtgaa cctcaggtgg
     2641 tcgtcttctc gagcagggcg tagaaaaatc cgtcggcggc cgcacggttc tcgggcacgg
     2701 ggcgctcggc tcgattgtcg ggcagcggca gcagatgccc gggcgatggt gggctggtgg
     2761 ccagggcggc accgggttgg cgttgcaaaa aagcgtcgat ctggtcccgg ccttcggcct
     2821 tgaacaccga acagctgcaa tacagcaggc gcccgccagg cgccagcagc ggccacagcg
     2881 catcgagcag ccgcgcctgg gccgccgcga gcgcggcgat gtcaccgggg cggcgcagcc
     2941 agcgcacgtc ggggtggcgg cgcacgatgc cggcggcgct gcacggggcg tcgagcagga
     3001 tcgcgtcgaa cggccggccg tcccaccagg cggccgggtc gccggcgtcg gcggcgacgc
     3061 tgcgcgcggt caggcccagg cgtgccagcg tctcgtggac gcgggtcagg cgcagcggat
     3121 cgctgtcgag cgcgagcacg tcgagatccg ccagctccag caggtgcgcg gtcttgccgc
     3181 ccggcgcggc gcaggcgtcg aggacgcgcg cgcccggcct caggccgcgc cgggccgccc
     3241 caagcggcct gagctcccct tgggggacga cgccgaaggc gtccaggggc tgtcgatcct
     3301 cgccggcgcc gatcagcagc ggcgcggcgc gctgggccgc ggcgtcctgc accgagacct
     3361 cgccctgcgc gaagccgggc agctgggtga cggggcaggg ttgcgccagc gcgaccgcct
     3421 gctcgcccag cgccgtcgcc gcgatgcctt gcgccagcag gcgcgcgacg tagctcggcg
     3481 catcgccgcg gcgtgcgttg acgcgcagcg tcatcggcgg gtgctggttg tcggcgtcga
     3541 ggatggcttg ccaatgctcg ggccaatcgg ccttcagccg ggcgatccac cagcgcggat
     3601 ggttgaagcg cgcgaccggg tcgcgcagcg cggccgcgac cagcgcgtcg cgctcgcgca
     3661 ggaagcggcg cagcaccgcg ttcacgaagg ccgcgctggg cttggcgcgc tggcgcgccg
     3721 catcgaccgc ctgatcgacc agcgtgtgct cggcgtaggg cgcttcggcc tgcggccaca
     3781 gcagcgccag cgcggtcagc agcagcgcgt cgaccgcc
//
LOCUS       DATMJB010000397         3435 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_313109, whole genome shotgun sequence.
ACCESSION   DATMJB010000397 DATMJB010000000
VERSION     DATMJB010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313109"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VNU48_08615"
     CDS             complement(<1..708)
                     /locus_tag="VNU48_08615"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HWI11375.1"
                     /translation="MARPRGRFAFGASASIGVGVLLALSADPACAAALGSVATSVGVL
                     VALAALFVLKGWPAHLPASALSTAGGVLVVAGAATWWWRARLAQRLAGFVAVPWRVGR
                     DDRPRAGGLGRAAAIALPADVGRETLLADMRRLFVQLQDAWDRHEMCALGRLTTPEML
                     AELCLELPGRSAGGELSCSTEVVTLHAELLGFETLAQDLVVSVEFSGLIREAADGGAV
                     PFRELWMLTRSKGGVEGW"
     gene            complement(764..1495)
                     /gene="ubiE"
                     /locus_tag="VNU48_08620"
     CDS             complement(764..1495)
                     /gene="ubiE"
                     /locus_tag="VNU48_08620"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HWI11376.1"
                     /translation="MSDTHFGFQTVDETQKAGRVRGVFDSVASKYDLMNDLMSMGLHR
                     AWKAYTVAVANLREGQTVLDIAGGTGDLARAFAKKVGAQGTVVHTDINEAMLRQGRDR
                     LLDEGLVLPTLICDGESLPFATATFDLVSVAFGLRNMTHKDTALGEMNRVLKPGGRLL
                     VLEFSKVAAPLAKAYDWYSFNVLPRIGQWVAGDAQSYRYLAESIRVHPGQAELKSMMK
                     AAGFGHVDVHNLSAGVVALHVGIKC"
     gene            complement(1510..1926)
                     /locus_tag="VNU48_08625"
     CDS             complement(1510..1926)
                     /locus_tag="VNU48_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HWI11377.1"
                     /translation="MAGLTANTPQPTSITVHQQSRVLEVGFADGAQFRIPFELMRVYS
                     PSAEVQGHGPGQEVLQTGKREVVLTALEPVGNYAVQPSFSDGHDTGIFSWDYLYFLGS
                     QQGELWRKYEERLAQAGVGRDAPMQAAAGAGCGHHH"
     gene            complement(2014..>3435)
                     /locus_tag="VNU48_08630"
     CDS             complement(2014..>3435)
                     /locus_tag="VNU48_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3400 domain-containing protein"
                     /protein_id="HWI11378.1"
                     /translation="DCLRCGKCKPVCATHVPRANLLYSPRNKILATSLLIEAFLYEEQ
                     TRRGVSIKHWEEFEDVADHCTVCHKCLSPCPVDIDFGDVSMNMRNLLGKMGKKSFRPG
                     NKMAMMFLNATNPETIKLLRIGMVDVGFKVQRIANNLLRGLANQQTAAPPATVGTAPL
                     KEQVIHFINKKMPGGLPKKTARALLDIEDKDYVPIIRDPGATTSETEAVFYFPGCGSE
                     RLFSQVGLATQAMLWHAGVQTVLPPGYLCCGYPQRGSGQFDKAEKIITDNRVLFHRVA
                     NTLNYLDIKTVVVSCGTCYDQLQGYQFDKIFPGCRIIDIHEYLLEKGITLGESSAGGY
                     LYHDPCHTPMKLQEPMKTVKALLGANVLESKRCCGESGTLGVTRPDISTQVRFRKEEE
                     LRKDEAALRRTGAVGAAGPVKILTSCPSCLQGLSRYTGDLTNGLLEADYIVVEMANQI
                     LGPDWMPEYVARANDGGIERVLV"
BASE COUNT          518 a         1230 c         1139 g          548 t
ORIGIN      
        1 ccagccctcg acgcccccct tcgaccgggt cagcatccac agttccctga acggcaccgc
       61 gccgccgtca gcggcctcgc ggatcaggcc cgagaactcg acgctgacca ccaggtcctg
      121 cgccagggtc tcgaacccga gcagctcggc atggagggtg acgacctcgg tcgagcagga
      181 tagttcgccg ccggcgctgc gccccggcag ttccaggcac agctcggcga gcatctcggg
      241 ggtcgtgagc cggccgagcg cgcacatctc gtgacgatcc cacgcgtctt gcaactgcac
      301 gaacaggcgg cgcatgtccg cgagcagcgt ctcgcggccc acgtcggccg gcaatgcgat
      361 ggccgccgcc cggccgaggc ctccggctcg cggccgatcg tcgcggccga cccgccacgg
      421 caccgccacg aacccggcca ggcgctgcgc caggcgtgcc cgccaccacc acgtggccgc
      481 gccggccacc accagcacgc cccccgccgt cgacagcgcg gacgccggca ggtgcgccgg
      541 ccagcccttg agcacgaaca gcgcggccag cgccacgagc acgccgaccg aggtcgcaac
      601 actcccgagc gccgcggcac acgccgggtc ggcgctcagc gcgagcagca ccccgacccc
      661 gatgctggcc gaagcaccga acgcgaaccg ccctcggggg cgcgccatgc cagcggcgat
      721 ggcagggtga cggagttgca tcgggcccgc cctcagccgc tgctcagcac ttgatgccga
      781 cgtgcagcgc gaccacgccg gcactcaggt tgtgcacgtc gacatggccg aagcccgccg
      841 ccttcatcat cgatttcagc tcggcctgcc ccgggtgcac gcggatcgac tcggccaggt
      901 agcgataact ctgcgcgtcg cccgccaccc actgcccgat gcgcggcagc acgttgaacg
      961 agtaccagtc gtaggccttc gccagcggcg ccgccacctt cgagaactcg agcaccagca
     1021 ggcggccgcc gggcttgagc acgcggttca tctcgcccag cgcggtgtcc ttgtgcgtca
     1081 tgttgcgcag gccgaaggcc acgctgacga ggtcgaacgt ggcggtcgcg aacggcagcg
     1141 actcgccgtc gcagatcagc gtcggcagca ccaggccctc gtcgagcagg cggtctcggc
     1201 cctggcgcag catcgcctcg ttgatgtcgg tgtggacgac ggtgccctgc gcgccgacct
     1261 tcttcgcgaa tgcgcgcgcc aggtcgccgg tgccgccggc gatgtcgagc acggtctggc
     1321 cctcgcgcag gttcgccacc gccacggtgt aggccttcca cgcgcggtgc aggcccatcg
     1381 acatcaggtc gttcatcagg tcgtacttcg acgcgaccga atcgaacacg ccgcgcacgc
     1441 ggccggcctt ttgcgtttcg tcgacggtct ggaagccgaa atgggtgtcg ctcatcggtt
     1501 gggcaccgtt cagtggtgat ggccgcagcc ggcgccggcc gcggcctgca tcggcgcgtc
     1561 gcggcccacg ccggcctgcg ccagccgctc ttcgtacttg cgccacagct cgccctgctg
     1621 ggaaccgagg aagtacaggt agtcccacga gaagatgccg gtgtcgtgac cgtcggagaa
     1681 actcggctgc accgcatagt tgccgaccgg ctccagcgcc gtcagcacga cctcgcgctt
     1741 gccggtctgc agcacttcct gcccggggcc gtggccctga acctcggccg agggcgagta
     1801 gacgcgcatc agctcgaacg ggatgcggaa ctgcgccccg tcggcgaagc cgacctccag
     1861 cacgcgcgac tgctggtgca ccgtgatcga ggtcggttgc ggggtgtttg cggtcaggcc
     1921 agccatgcgt ttcgttctgt ggggtgaagc cgccgcagtg tagcgaagcg ccctgccccg
     1981 cacggcggcg gggccttggg ggccgcgtgg gtactacacc agcacgcgct cgatcccgcc
     2041 gtcgttggcg cgcgccacgt actcgggcat ccagtcgggg ccgaggatct ggttcgccat
     2101 ctcgacgacg atgtagtcgg cctccagcag cccgttggtc agatcgccag tgtatcgact
     2161 cagcccctgc aggcagctcg ggcacgacgt gaggatcttc accggcccgg ccgcgccgac
     2221 cgcgccggtg cggcgcagcg cggcctcatc cttgcgcagt tcctcttcct tgcggaagcg
     2281 cacctgcgtc gaaatgtcgg gccgcgtgac cccgagcgtg ccgctctcgc cgcagcagcg
     2341 cttgctctcg agcacattgg caccgagcag cgccttcacg gtcttcatcg gctcctgcag
     2401 cttcatcggc gtgtggcagg ggtcgtgata aaggtagcca ccagcggacg actcacccag
     2461 cgtgatgccc ttctccagca ggtactcgtg gatgtcgatg atccggcagc cggggaagat
     2521 cttgtcgaac tggtagccct gcagctggtc gtagcaggtg ccgcagctca ccaccacggt
     2581 cttgatgtcg aggtagttca gcgtgttcgc gacgcggtgg aacagcaccc ggttgtcggt
     2641 gatgatcttc tcggccttgt cgaactggcc cgagccgcgc tgcggatagc cgcagcacag
     2701 gtagccgggc ggcagcaccg tctgcacgcc ggcgtgccac agcatcgcct gcgtcgccag
     2761 gccgacctgc gagaacagcc gctccgagcc gcagcccggg aagtagaaga cggcctcggt
     2821 ctcgctcgtc gtggcccccg ggtcgcggat gatcggcacg tagtccttgt cctcgatgtc
     2881 gagcagcgcg cgcgccgtct tcttcggcag gccgcccggc atcttcttgt tgatgaagtg
     2941 gatcacctgc tccttgagcg gggcggtgcc gaccgtcgcc ggcggcgcgg cggtctgctg
     3001 attcgccagg ccgcgcagca agttgttcgc gatgcgctgc accttgaagc cgacatcgac
     3061 catgccgatg cgcagcagct tgatcgtctc cggattcgtg gcgttcagga acatcatcgc
     3121 catcttgttg ccgggccgga agctcttctt gcccatcttg ccgagcaggt tgcgcatgtt
     3181 catcgacacg tcgccgaagt cgatgtccac cgggcacggg ctcaggcact tgtggcagac
     3241 ggtgcagtgg tcggccacgt cctcgaactc ttcccagtgc ttgatcgaca cgccccggcg
     3301 cgtctgctct tcatagagaa acgcctcgat cagcagcgag gtcgcgagga tcttgttgcg
     3361 cgggctgtag agcaggttgg cacgcggcac gtgcgtcgcg cagaccggct tgcacttgcc
     3421 gcagcgcagg cagtc
//
LOCUS       DATMJB010000398         2019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_185830, whole genome shotgun sequence.
ACCESSION   DATMJB010000398 DATMJB010000000
VERSION     DATMJB010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185830"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VNU48_08635"
     CDS             <1..254
                     /locus_tag="VNU48_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HWI11379.1"
                     /translation="RKHKSAYLMAVGGAAYLVSKAIKHAAVVGFADLGMEAIYEFDVT
                     DMPVTVAVDSHGVSVHDTGPRDWEARIRSGSFRIPVASA"
     gene            285..1094
                     /locus_tag="VNU48_08640"
     CDS             285..1094
                     /locus_tag="VNU48_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter"
                     /protein_id="HWI11380.1"
                     /translation="MSATDLPHDVASLALVILLLGMKHGFDADHLAAIDGLTRYNAAS
                     RPRLARAAGVLFSAGHGLVVVAVAVGVALLARAWRVPEWLEAFGAWVSIALLTLLALL
                     NIVAVLRTPGHQVARLEGWRSGAFAWLLRAGNPLMVMGVGTLFALSFDTVGQAALFAV
                     TASRFGGWQPALLLALLFVAGMLATDGVNGLWIARLIRRSDRSARVASRVLALAVSGV
                     GLLTAALTFASRTLPDVNAWAQGKELWFGAAVVAVVGASFAFGQYLARAEA"
     gene            1142..>2019
                     /locus_tag="VNU48_08645"
     CDS             1142..>2019
                     /locus_tag="VNU48_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HWI11381.1"
                     /translation="MSRSAAQPFARDRRAPARVTNEELFFDLIYAFAITQLSHRLLEQ
                     LTPLGALQTLVLWFAVWLGWQYTCWVTNWFNPENLRMRLMLFALMLLGLAMGATIPAA
                     FAGAGLAFAACYAAIQVGRTLFVLLNLRAAPALRPNFRRIMGWVCISAALWIAGGLSD
                     GSTRLTLWIAAVASEYLAPMIGFALPVLGRSRTSEWTIEGGHLAERNQLFVMIALGES
                     ILATGASFSQSPHLDAPTLIALAVAFAGSVAMWWIYFDTASEHGTHAITGSDDPGRIG
                     AYFHYVHVTLIAGIIVA"
BASE COUNT          243 a          711 c          728 g          337 t
ORIGIN      
        1 tcaggaagca caagagcgcg tacctgatgg cggtgggcgg cgcggcctac ctggtgtcga
       61 aggcgatcaa gcacgccgcg gtggtcggct tcgccgacct gggcatggaa gcgatctacg
      121 agttcgacgt gaccgacatg ccggtcacgg tggccgtcga ttcgcacggc gtgtcggtcc
      181 acgacaccgg gccgcgcgac tgggaggcga ggatccgctc gggctcgttc aggatcccgg
      241 tcgcgagtgc ctgaggtcgg aacctcgcgg cccgctgggc ggcgatgagc gcgaccgacc
      301 ttccgcacga cgtcgccagc ctggcgctgg tgatcctgct gctgggcatg aagcacggct
      361 tcgacgccga ccacctggcc gcgatcgacg ggctgacgcg ctacaacgcc gcgtcgcggc
      421 cgcgcctggc gcgggcggcg ggcgtgctgt tctcggccgg gcacggcctc gtggtcgtcg
      481 ccgtggccgt cggcgtcgcg ctgctggcgc gcgcctggcg cgtgcccgaa tggctggagg
      541 cgttcggcgc gtgggtctcg atcgcgctgc tcacgctgct ggcgctgctg aacatcgtcg
      601 cggtgctgcg caccccgggt caccaggtcg ctcggctgga aggctggcgc agcggcgcgt
      661 tcgcgtggct gctgcgcgcc ggcaacccgc tgatggtgat gggcgtcggc acgctgttcg
      721 cgctgtcgtt cgacaccgtg ggccaggccg cgctgttcgc cgtcaccgcg agccggttcg
      781 gcggctggca gccggcgctg ctgctggcgc tgctgttcgt cgccggcatg ctcgcgaccg
      841 acggcgtcaa cgggttgtgg atcgcgcgcc tgatccgccg cagcgaccgc agcgcgcgcg
      901 tggcgtcgcg cgtgctggcg ctcgccgtct cgggggtcgg gctgctgacc gccgcgctga
      961 ccttcgcgag ccgcacgctg cccgatgtga acgcgtgggc gcagggcaag gagctctggt
     1021 tcggcgccgc ggtggtcgcc gtcgtcggcg ccagcttcgc cttcggtcag tacctcgcgc
     1081 gcgccgaggc ctgatcgcgc ccgtcgctaa catcgcgcgg tcgaatcggg gagaacgaac
     1141 gatgagccgc agcgccgccc aacccttcgc gcgcgaccgc cgcgcccctg cccgggtcac
     1201 caacgaagag ctgttcttcg acctgatcta cgcgttcgcg atcacccagc tgtcgcaccg
     1261 cctgctcgag cagttgacgc cgctcggcgc gctgcagacg ctggtgctgt ggttcgcggt
     1321 ctggctcggc tggcaataca cctgctgggt cacgaactgg ttcaaccccg agaacctgcg
     1381 catgcggctg atgctgttcg cgctgatgct gctcggcctg gcgatgggtg cgacgatccc
     1441 ggcggccttc gccggggccg ggctcgcgtt cgcggcctgc tacgccgcga tccaggtcgg
     1501 gcgcacgctg ttcgtgctgc tcaacctgcg cgccgcgccg gcgctgcggc cgaactttcg
     1561 ccgcatcatg ggctgggtct gcatctcggc ggcactgtgg atcgccggcg gcctgtcgga
     1621 cgggtcgacc cggctgacgc tgtggatcgc cgccgtcgcg agcgaatacc tcgcaccgat
     1681 gatcggcttc gcgctgccgg tgctcggccg ctcgcgcacc agcgaatgga cgatagaagg
     1741 cggccacctc gccgaacgca atcagctgtt cgtgatgatc gcgctcggcg aatcgatcct
     1801 cgcgaccggc gcctcgttca gccagtcgcc gcatctcgat gcgccgacgc tgatcgcgct
     1861 ggcggtggcg ttcgccggca gcgtcgcgat gtggtggatc tacttcgaca ccgcgagcga
     1921 gcacggcacg catgcgatca ccggctccga cgacccgggc cgcatcggcg cgtacttcca
     1981 ctacgtccac gtgaccctga tcgccggcat catcgtcgc
//
LOCUS       DATMJB010000399         4096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_596866, whole genome shotgun sequence.
ACCESSION   DATMJB010000399 DATMJB010000000
VERSION     DATMJB010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596866"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VNU48_08650"
     CDS             complement(<1..355)
                     /locus_tag="VNU48_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11382.1"
                     /translation="MSDLRIAAPHDRRGRATRVRVNLVSLVLAPGVGIAGYLLGGWPG
                     VLTAEGLWAGAVFAGTLIHWLPHSAGRLGAIHLEPHCDRNRWLDRFSVRLQELRPSMS
                     FPVAAKFALRTWPEAA"
     gene            656..1189
                     /locus_tag="VNU48_08655"
     CDS             656..1189
                     /locus_tag="VNU48_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWI11383.1"
                     /translation="MTSLAASRSSVAERAAASTGHAQEYLTLCLGDEEYGIDILCVQE
                     IRGYEPPTRIAGVPDFIRGVLNLRGVIVPIVDLRLRLGVESRFDAVTVTIVLNVAGRT
                     VGAVVDSVSDVIELARTQIKPTPEFNGAIDAGHITGLGTVRQGERERMLILLDIEKLM
                     ASANLGLVEQATQGDNP"
     gene            1186..2799
                     /locus_tag="VNU48_08660"
     CDS             1186..2799
                     /locus_tag="VNU48_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWI11384.1"
                     /translation="MMFGNLTVRGKLTAAFGGLAVLVLLVAGLSLKALSDGNDRFVGY
                     VTGLNVRATLASQLLAAIDRRAIAARNLVLVTTPEDLEVEQALVTKAHEDVQEHLNRL
                     TKMIADAPDSTDKASALISDMGKIESAYGPVALAIVDLALNKKHDEAIAKMNKECRPL
                     LAAMVKVSNEYRDHTASRAEARTAESAASYSAQRNLLVLACVGAFAAAAIAGVLITRG
                     LTRALGAEPADLGRAAQRIASGDLSPVAGAARAPKGSVMVSLGAMQDSLASIVGQVRN
                     SSDSIATGSAQIATGNADLSQRTEEQASALQQTAATMDELGSTVRHNADNAAQANQLA
                     LGASAVAARGGDVVSQVVGTMKGINDSSKKIADIISVIDGIAFQTNILALNAAVEAAR
                     AGEQGRGFAVVASEVRSLAQRSAEAAKEIKVLITDSVVQVEQGTALVDQAGHTMEEIV
                     AAIKRVSDIVAEISSASVEQSSGVTQVGHAVSQMDQVTQQNAALVEESAAAAESLKQQ
                     AEQLVHAVALFKLAQGGSAATAPVAASIT"
     gene            complement(2897..2971)
                     /locus_tag="VNU48_08665"
     tRNA            complement(2897..2971)
                     /locus_tag="VNU48_08665"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2936..2938),aa:Arg,seq:cct)
     gene            3170..3373
                     /locus_tag="VNU48_08670"
     CDS             3170..3373
                     /locus_tag="VNU48_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11385.1"
                     /translation="MNGRRVDATADRQNPWGWAPRVPGEQAVAIEPRRLRIARLLAMF
                     TRADTAKHEAFVDTVPGVMFDRE"
     gene            complement(3396..>4096)
                     /locus_tag="VNU48_08675"
     CDS             complement(3396..>4096)
                     /locus_tag="VNU48_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HWI11386.1"
                     /translation="ALDLRVGLGEKLRVRGRGLDTGLRGELHITSPGGKLAVDGTVRA
                     VDGTYAAYGQKLSIDRGLITFSGPVENPRLDIEATRPNLDVRVGVAVSGSTANPRVRL
                     FSEPELSEIDKLSWLVMGRASDGLGRTDTALLQRAALALLAGEGGGPTDRITKALGLD
                     DISVRQTDGEVRETVIALGKQLSKRWYVGYERGLNATAGSFQLIYRIARRFTLRAQSG
                     DDNSMDLIWTWRWQ"
BASE COUNT          723 a         1367 c         1402 g          604 t
ORIGIN      
        1 cggctgcctc cggccaagtg cgcagcgcga acttggcggc cactggaaag ctcatcgacg
       61 gccgcagttc ctgcagccgg acactgaaac gatcgagcca tcggttcctg tcgcaatgcg
      121 gctcgaggtg gatggcgccg agcctgcctg cggagtgagg cagccaatgg atgagcgtgc
      181 ccgcgaacac tgcacctgcc cagagcccct cggccgtgag gacaccgggc cagccaccca
      241 agaggtagcc ggcgatgcca acgcccgggg ccagcaccag cgagaccagg ttgaccctca
      301 ccctggtggc ccgcccacgt cgatcatgag gtgccgcgat gcgtaagtcg ctcatcgtga
      361 tcgctccttc ggattgcgct gacggatcgt cctgcaagct cggcgcgcct ggcgccggaa
      421 ggaatcgcgt ccttgtgcca gcggcgtctt acacgccggc gcgcaagtcg ccgctgcctg
      481 caccgatgcg gttccgggct atctggcatt cagcagggcc gctgacgaga ttcggaaacg
      541 gactacccaa gattcagcga cacctgactg caattgtcag tggccgccga agaacaagtc
      601 gccggatttc gccgaaatca agggtgtgca attccaacca cccggcgagc cttcaatgac
      661 cagccttgca gcctcgagat ccagcgtcgc ggagcgtgcc gcggcaagca ccggccacgc
      721 gcaagagtat ctgaccctgt gcctcggcga cgaggaatac ggcatcgaca tcctgtgcgt
      781 acaggagatc cgtggatacg agccgcccac gcggatcgcc ggtgtaccgg acttcatccg
      841 cggcgtactc aacctgcgcg gcgtgatcgt gcccatcgtg gatcttcggc tgcgccttgg
      901 tgtcgagtcg cggttcgacg cggtcaccgt cacgatcgtg ctcaatgtgg cggggcgcac
      961 cgtgggagca gtcgtcgatt cggtgtccga cgtgatcgaa ctggcccgca cgcaaatcaa
     1021 gcccaccccg gaattcaacg gcgcgatcga cgccggccac atcaccggct tggggacggt
     1081 ccgtcaaggc gagcgcgaac gcatgctcat cctgctggac atcgagaaac tgatggccag
     1141 cgccaatctc ggtctggtcg aacaagcaac gcaaggagac aacccatgat gttcggcaat
     1201 ctgacggtgc gcggcaagct gaccgcggcg ttcggcggcc tcgccgttct ggtcttgctc
     1261 gtggccgggc tgtcgttgaa ggccttgagc gacgggaacg accggttcgt gggctacgtc
     1321 acgggtctca acgtgcgcgc cacgctggcc tcgcagctgc tcgctgcgat cgaccgccgc
     1381 gccatcgcgg cgcgcaacct ggtgctcgtg accacgcccg aggacctcga ggtggaacag
     1441 gccttggtca ccaaggccca cgaagacgtg caggagcacc tgaacaggct cacgaagatg
     1501 atcgccgacg cgccggactc gacggacaag gcaagcgcgt tgatcagcga catggggaag
     1561 atcgagagcg cgtacggccc cgtcgccctg gccatcgtcg atctcgcgct caacaagaag
     1621 cacgacgagg cgatcgccaa gatgaacaag gaatgccgcc cgctgctggc cgcgatggtg
     1681 aaggtctcga acgagtatcg cgaccacacg gccagccggg ccgaggcgag gacggcagaa
     1741 tcagccgcca gctattcggc gcagaggaac ctgctcgtcc tggcctgcgt cggcgccttc
     1801 gcggcggcgg cgatcgccgg tgtgctcatc acccgcgggc tgacccgcgc gcttggcgcc
     1861 gagcccgccg acctgggccg ggcggcgcaa cgaatcgcca gcggcgacct gagcccggtg
     1921 gccggcgccg cgagggctcc gaagggcagc gtgatggtgt ctctcggcgc catgcaggac
     1981 agccttgcca gcatcgtcgg ccaggtgcgc aactcgtccg actcgatcgc cacgggttcc
     2041 gcacagatcg ccaccggcaa cgccgatctc agccagcgca ccgaagagca ggccagcgca
     2101 ctgcagcaga ccgccgcgac gatggacgaa ctcggatcga cggtgcgcca caacgccgac
     2161 aacgccgcgc aggcgaacca gctggcgctg ggtgcatcgg cggtggccgc aaggggcggc
     2221 gacgtggtga gccaggtggt gggcacgatg aagggcatca acgacagctc caagaagatc
     2281 gccgacatca tcagcgtgat cgacggcatc gccttccaga ccaacatcct ggcgctcaac
     2341 gccgccgtgg aagccgcgcg ggccggcgag cagggccgcg gcttcgcggt cgtcgccagc
     2401 gaggttcgca gcctggcgca gcgcagcgcc gaagcggcca aggaaatcaa ggtgctgatc
     2461 accgacagcg tcgtgcaggt cgagcaggga acggcgctgg tcgatcaggc tggtcacacg
     2521 atggaagaga tcgtcgctgc gatcaagcgc gtcagcgaca tcgtcgcaga gatcagttcg
     2581 gccagcgtcg aacagagttc gggcgtgacc caggtcggcc acgcggtgag ccagatggac
     2641 caggtcacgc agcagaatgc cgcgctcgtc gaggagagcg cggcggcggc cgagagcctg
     2701 aagcagcagg cggagcaact cgtgcacgcc gtggcgctgt tcaagctcgc gcagggcggg
     2761 tctgcggcca ctgcgccggt ggctgcgagc attacctgat cttgaacgcg acgaacgact
     2821 gaaagttgaa acgatgcgag acgtgcagca gacgctgcgc gtctcgcctg ggacggatct
     2881 cttgcgacaa gctcgttggt cccgccgaca ggaatcgaac ctgtatctgc cgcttaggag
     2941 gcggccgttc tgtccattga actacggcga gccgggcgca cggggcaccc gtcagcagcg
     3001 tcggattgtc gccgactcgg gcgcgcatcg aaggccggga cgcccggtgg cgcgccggcg
     3061 cggcgacgaa tgtcgtcgat tcctacaatg agccgagaaa ccgttacttg gcgggcgcct
     3121 tgccgatccg gatcattgcc tacattcgag attcgtggag cattcgcaca tgaacggcag
     3181 gcgggtcgac gcgacggcgg atcggcaaaa tccgtggggc tgggccccgc gcgtccccgg
     3241 cgagcaggcg gtcgcgatcg agccgcgcag gttgcgcatc gcgcgcttgc tggcgatgtt
     3301 cacgcgggcc gacaccgcga agcacgaagc gttcgtggac acggtgcccg gcgtgatgtt
     3361 cgatcgcgag tgaggcggcg gcacggccgg cgtggctact gccagcgcca ggtccagatc
     3421 aggtccatcg agttgtcgtc gcccgattgc gcgcgcaggg tgaagcgccg cgcgatgcgg
     3481 tagatcagct ggaagctgcc ggccgtcgcg ttcaggccgc gttcgtagcc gacgtaccag
     3541 cgcttggaca actgcttgcc cagcgcgatc accgtctcgc ggacctcgcc gtcggtctgg
     3601 cgcaccgaga tgtcgtccag ccccagcgcc ttcgtgatcc ggtcggtcgg cccgccgcct
     3661 tcgccggcca gcagcgcgag cgcggcgcgt tgcagcagcg cggtgtcggt gcgccccagg
     3721 ccgtcgctgg cacggcccat cacaagccag ctgagcttgt cgatctccga cagttcgggc
     3781 tccgagaaca gccgcacgcg cgggttcgcg gtgctgccgc tgaccgcgac gccgacgcgc
     3841 acgtccaggt tcggccgtgt cgcctcgatg tccagccgcg ggttctcgac cgggccgctg
     3901 aaggtgatca ggccgcggtc gatgctcagc ttctggccat aggccgcgta ggtgccgtcg
     3961 accgcgcgca ccgtgccatc gaccgcgagc ttgccgcccg gcgaggtgat gtgcagctcg
     4021 ccgcgcaggc ccgtgtccag gccgcgcccg cgcacgcgca gcttctcgcc caggcccacg
     4081 cgcaggtcga gcgcga
//
LOCUS       DATMJB010000400         4290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_48232, whole genome shotgun sequence.
ACCESSION   DATMJB010000400 DATMJB010000000
VERSION     DATMJB010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48232"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..2541
                     /locus_tag="VNU48_08680"
     CDS             157..2541
                     /locus_tag="VNU48_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWI11387.1"
                     /translation="MLPVSDHVDTGNSPLLRHLNPEQLAAVTLPARPALILAGAGSGK
                     TRVLTTRIAWLIQQGMLSPGGVMAVTFTNKAAKEMLTRLGAMLPINVRGMWIGTFHGL
                     CNRFLRAHWKLAALPQGFQILDSADQLSAVKRVIKAMNLDEERFVPKQVTWFIAGAKD
                     NGYRPKDCEVKDELSRVQVQIYQAYEDQCQREGVVDFAELMLRSYELMRDNEALRQHY
                     QHRFRHILVDEFQDTNRLQYAWLKMFAGPQTAVFAVGDDDQSIYAFRGAQVGNMAAFE
                     REFHVDHVIKLERNYRSFGNILDAANELIARNTNRLGKNLRTEAGPGEPVRVRESTSD
                     FDEARWFVEEAQQLHREGTARSDIALLYRSNAQSRVMESALFNAGIPYKVYGGLRFFE
                     RAEVKHALSYLRLIENPGDDTSFLRVVNFPTRGIGARTIELLQDAARTSGRGLYQSVG
                     ALGGKGGGNLQAFVALIDGMRETTRGMTLRDIIEHMLRASGLVEFYKTDKEGRDRIEN
                     LDELVNAAEAFVTQEGFGKDAVALPVDEHGREAGRAEFSTPGAQATAGRIVTPLPELA
                     PDAETGEIMSPLAAFLTHASLEAGDNQAQAGQDAIQLMTVHSAKGLEFDCVFITGLEE
                     GLFPHENSAADPDGLEEERRLMYVAITRARKRLYLSFAQTRMLHGQTRYNVKSRFLDE
                     LPEGALKWITPRNQGFGSGFQRDYQAAWARGTGLHSIVGAGNVSGLPSAPPLVPKAPS
                     HGLRSGQNVFHTKFGEGVVITLEGSGVDARAQVNFGRHGMKWLALSVAKLTPID"
     gene            2592..3206
                     /locus_tag="VNU48_08685"
     CDS             2592..3206
                     /locus_tag="VNU48_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11388.1"
                     /translation="MPYSLRSSCATLLAATGLLLSGCASTGVDAQWRSVELPPGYLHG
                     ATVLVSCETGELVLQRICEDRVVADLAARGVRAVLSAPNAAPAAPAGVTDMQYVPVAR
                     DAGAKAVFSVSVGVASQMVNPGVSLGLGVGGFGRYSAGGIGVSAPVGGGQVNSGYAMS
                     GRVTDVASGRLMWTARAIAPPSSDANAQLAELSKSLLDAAAGLF"
     gene            complement(3203..>4290)
                     /locus_tag="VNU48_08690"
     CDS             complement(3203..>4290)
                     /locus_tag="VNU48_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWI11389.1"
                     /translation="HVDPSFDVDGLPRRLDVPAHSRLEVTYQLTPRRRGPVHFAPAEL
                     RVRSLLGSFELLRRVGVDEALRVYPNFAAVARYAWLASDRRLAEIGIKAAPPRGGGTD
                     FKQLAEYQSGDSIRHIDWKATLRHRRAIVREYQDERDQTVLFLLDCGRRMRADEGAAE
                     GSGSERGSGGSHFDEALNALMLLAHVALSEGDAVGAMTFGTADADARHFAPRKGKTSL
                     NALIAAVYDIEPRATHSDYLGAASALMRVQHKRALVVVLTNFRDEDSAEIEPALRLLR
                     TRHLVLLASLRERVLRELAEQPLGTSVASDAKAIEVAGAHLFAQSRRDAFARLATRDG
                     LLIDVEPAQLPAQLVNRYRAVKRAGLL"
BASE COUNT          651 a         1481 c         1545 g          613 t
ORIGIN      
        1 ccgagggcgg caggggcggg cagcaggggt cggacatcgt gcgggctttg gcgggagggt
       61 cgtcgcgact atagaggtgg gcccgcgcgt tcggggacga tgtggcgaat cgccgccgtc
      121 gcgacggggc cgccggccaa ccccgacaat cacgtcatgc ttcccgtctc cgaccacgtc
      181 gacaccggca actcgccgct gctgcgccac ctgaaccccg aacagctcgc cgcggtgacg
      241 ctgccggcgc gcccggcgct gatcctggcc ggtgccggtt cgggcaagac ccgcgtgctg
      301 accacgcgca tcgcctggct gatccagcag ggcatgctgt cgcccggcgg ggtgatggcg
      361 gtgaccttca cgaacaaggc cgcgaaggag atgctgacgc gcctcggcgc gatgctgccg
      421 atcaacgtgc gcggcatgtg gatcggcacc ttccacggcc tgtgcaaccg cttcctgcgc
      481 gcgcactgga agctcgccgc gttgccgcag ggcttccaga tcctcgattc ggccgaccag
      541 ctctcggccg tcaagcgcgt catcaaggcg atgaacctcg acgaggagcg cttcgtgccc
      601 aagcaggtga cctggttcat cgccggcgcg aaggacaacg gctaccgccc gaaggactgc
      661 gaggtcaagg acgagctctc gcgcgtgcag gtgcagatct accaggccta cgaggaccag
      721 tgccagcgcg aaggcgtggt cgacttcgcc gagctgatgc tgcgcagcta cgagctgatg
      781 cgcgacaacg aggcgctgcg tcagcactac cagcaccggt tccgccacat cctcgtcgac
      841 gagttccagg acacgaaccg gctgcagtac gcgtggctga agatgtttgc cgggccgcag
      901 accgcggtgt ttgcggtcgg tgacgacgac cagagcatct acgccttccg cggcgcgcag
      961 gtcggcaaca tggcggcgtt cgagcgcgag ttccatgtcg accacgtgat caagctggaa
     1021 cgcaactacc gctcgttcgg caacatcctc gacgcggcca acgagctgat cgcgcgcaac
     1081 acgaaccgcc tcggcaagaa cctgcgcacc gaggccgggc cgggcgagcc ggtgcgggtg
     1141 cgcgagtcga ccagcgactt cgacgaggcg cgctggttcg tcgaagaggc gcagcagctg
     1201 caccgcgagg gcacggcgcg cagcgacatc gcgctgctgt accgcagcaa cgcgcagagc
     1261 cgcgtgatgg agagtgcgct gttcaacgcc ggcatcccgt acaaggtcta cggcgggctg
     1321 cgcttcttcg agcgcgccga ggtcaagcac gcgctgagct acctgcggct gatcgagaac
     1381 ccgggcgacg acaccagctt cctgcgcgtc gtcaacttcc cgacacgcgg catcggcgcg
     1441 cgcacgatcg agctgctgca ggacgcggcc cgaaccagcg ggcgcggcct gtaccagagc
     1501 gtcggcgcgc tcggcggcaa gggcggcggc aacctgcagg ccttcgtcgc gctgatcgac
     1561 ggcatgcgcg agaccacgcg cggcatgacc ttgcgcgaca tcatcgagca catgctgcgc
     1621 gccagcgggt tggtcgagtt ctacaagacc gacaaggaag gccgcgaccg gatcgagaac
     1681 ctcgacgaac tcgtcaacgc ggccgaggcc ttcgtcacgc aggaaggttt cggcaaggac
     1741 gcggtcgcgc tgccggtgga cgagcacggc cgcgaagccg gccgcgccga gttcagcaca
     1801 ccgggcgcgc aggccaccgc gggccggatc gtcacgccct tgcccgagct cgcgcccgac
     1861 gccgagaccg gcgagatcat gtcgccgctc gccgcgttcc tgacccacgc atccctcgag
     1921 gccggcgaca accaggcgca ggccgggcag gacgcgatcc agctgatgac ggtgcattcc
     1981 gccaagggcc tggagttcga ctgcgtgttc atcaccgggc tcgaggaagg cctgttcccg
     2041 cacgagaact cggccgccga ccccgacggg ctcgaggagg agcgccgcct gatgtacgtc
     2101 gcgatcacgc gcgcgcgcaa gcgcctctat ctgagcttcg cccagacacg catgctgcac
     2161 ggccagacgc gctacaacgt caagagccgc ttcctcgacg agctgccgga aggcgcgctg
     2221 aagtggatca cgccgcgcaa ccagggtttc ggctcgggct tccagcgcga ctaccaggcg
     2281 gcctgggcga ggggcacggg gctgcactcg atcgtcggcg ccgggaatgt cagcgggctg
     2341 ccgagcgcgc cgccgctcgt gccgaaggcg ccgagccacg gtctgcgctc gggtcagaac
     2401 gtgttccaca ccaagttcgg cgagggcgtg gtcatcacgc tcgaaggcag cggcgtcgat
     2461 gcgcgcgcgc aggtcaactt cggccgccac ggcatgaagt ggctcgcgct gtcggtcgcg
     2521 aaactaacgc cgatcgactg agtccatcgc cggttgtcac tgcgtcgttc atgggcccga
     2581 aggagatcct catgccgtac tcgctgcgaa gctcttgcgc gacgctgctc gccgccaccg
     2641 ggctgctgct gtcgggctgt gccagcaccg gggtcgacgc gcagtggcgc agcgtcgaac
     2701 tgccgccggg ctacctgcac ggggcgaccg tgctggtgag ttgcgagacc ggcgagctgg
     2761 tactgcagcg catctgcgag gaccgggtcg tcgccgatct ggctgcgcgc ggcgtgcgcg
     2821 cggtgctgtc cgcgccgaac gccgcgccgg cggcgccggc gggcgtcacc gacatgcagt
     2881 acgtgccggt cgcgcgcgac gccggcgcca aggcggtgtt cagcgtcagc gtcggcgtgg
     2941 cttcgcaaat ggtcaacccg ggggtctcgc tcggtctggg ggtgggcggc ttcggccgct
     3001 attcggccgg cggcatcggc gtgtcggcgc cggtgggtgg cggccaggtg aattcgggct
     3061 acgcgatgag cggccgtgtc accgatgtcg cgtcgggccg gctgatgtgg accgcgcgcg
     3121 cgattgcacc gccgtcgtcg gacgcgaacg cgcagctcgc cgagctgtcg aagagcctgc
     3181 tcgacgcggc ggccggattg ttctagagca gcccggcgcg cttgaccgcg cggtagcggt
     3241 tcaccagctg cgcgggcagc tgcgccggct cgacgtcgat cagcaggccg tcacgcgtcg
     3301 cgaggcgcgc gaacgcgtcg cggcgcgact gcgcgaacag gtgcgcgccg gccacctcga
     3361 tcgccttcgc gtcgctggcg acactggtgc ccagcggctg ctcggcgagc tcgcgcagca
     3421 cgcgttcgcg caggctcgcg agcagcacca ggtggcgtgt gcgcagcagc cgcagcgccg
     3481 gctcgatctc ggcgctgtcc tcgtcgcgga agttcgtcag caccacgacc agcgcgcgct
     3541 tgtgctgcac gcgcatcagc gcgctcgcgg cgccgaggta gtcggagtgg gtcgcgcgcg
     3601 gttcgatgtc gtagaccgcg gcgatcagcg cgttcagcga ggtcttgccc ttgcgcggtg
     3661 cgaagtggcg cgcgtcggcg tcggcggtgc cgaaggtcat cgcaccgacc gcgtcgccct
     3721 cgctcagcgc cacgtgcgcg agcagcatca gcgcgttcaa cgcctcgtcg aagtggctgc
     3781 cgccgctgcc gcgctccgac cccgatcctt cggcagcgcc ttcgtcggcg cgcatgcggc
     3841 gcccgcagtc gagcaggaac agcaccgtct ggtcgcgttc gtcctggtac tcgcgcacga
     3901 tcgcgcgccg atgccgcagc gtcgccttcc agtcgatgtg gcggatcgag tcgccgctct
     3961 ggtactcggc gagctgcttg aagtcggtgc cgccgccgcg cggcggcgcg gccttgatgc
     4021 cgatctcggc caggcggcgg tcgctggcga gccaggcgta gcgtgcgacc gcggcgaagt
     4081 tcgggtagac gcgcaaggcc tcgtcgacac ccacgcggcg cagcagctcg aagctgccga
     4141 gcaggctgcg cacgcgcagc tcggccggcg cgaagtgcac cgggccgcgg cgccgcggcg
     4201 tcagctggta cgtgacctcg agccgcgagt gtgccggcac gtcgaggcgg cgcggcaggc
     4261 cgtcgacatc gaagctcggg tcgacgtggt
//
LOCUS       DATMJB010000401         2928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_241529, whole genome shotgun sequence.
ACCESSION   DATMJB010000401 DATMJB010000000
VERSION     DATMJB010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241529"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1173)
                     /locus_tag="VNU48_08695"
     CDS             complement(154..1173)
                     /locus_tag="VNU48_08695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWI11390.1"
                     /translation="MRYTEPKERSAELLRVALGHMGRHEAAFNPVTFTLWYEYAAGIN
                     PTLHEAVEQRLQDSVPFSDTVVAQLYQDHIVPPDAATVQRIGGELQRVMSGIASRASQ
                     TGTDAHRFGAQLDDLSAALRSSDTAALSPHLDQARAGTAEMKSSAAALQQQVSASQTE
                     IARLRSDLDRARGEALLDPLTSILNRKGFDQRLAALLQRPPAAGQQHCLVMLDIDHFK
                     KVNDAHGHVMGDRVIQGLGEILRLTVTDPEHATARYGGEEFAIVLPHSTLTEAARLAE
                     QVRLRAKAMRIRNRSTQEVLFTVTISGGVAALHPGDDAQALIARADAALYQSKNGGRD
                     RVSCA"
     gene            complement(1235..>2928)
                     /locus_tag="VNU48_08700"
     CDS             complement(1235..>2928)
                     /locus_tag="VNU48_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HWI11391.1"
                     /translation="AGHTTTRDHVVLNVLEDVASRLEEWQRTPQGFVRRAIDAPFPGT
                     LTAAGLHDPARVDDPLADHYLLGYSDFLTPDALWLARAGSDARETLKALPPRFDASGM
                     HVDQFFATSADGTRVPYFVVWPAGVDAARPGDGSTPTLLYGYGGFEISMQPWYAAGYG
                     RGWLARGGVLVVANIRGGGEYGPAWHQAALKAHKQRSYDDFIAVAQDLIARRITSPAR
                     LGIEGGSNGGLLVGAAFTQRPELFGAVVCSVPLLDMRRYHRLLAGASWMAEYGNPDLP
                     EEWAWIARYSPYQNLQPGRRYPEVLFTTSTRDDRVHPGHARKMAAKMLALGQPVLYYE
                     NIEGGHGGAADNAQRAHVQALEMTYLWHRLGGTAVADAGALPADAQAVLDYWFGADDG
                     PERDFWFTKSEATDREIAQRFGALIERALRGELADWAAQPRAALAQILLLDQFTRNVF
                     RDTPRAFAGDARALRAATAMVGARHDEALPVQWRAFVYLPFEHAEGLGAQDEAVRLFT
                     RLAAEAPELQPMLAYAHKHRAVVERFGRFPHRNAILGRQSTPEEVAFLREPGSRF"
BASE COUNT          391 a         1037 c         1064 g          436 t
ORIGIN      
        1 aggggttggc cgcaggccgg ccccggggga catgagcgca gcagcatgcc ccgccggtcc
       61 gatcccgcgc ccatacgtgc acggcggaca ttcaccgaca gcatcgggcc gccagccgac
      121 acagcatcac aactccatac cgccagtttc acctcaggcg cagctgacgc ggtcgcgccc
      181 gccgttcttc gactggtaca gcgcggcgtc ggcgcgcgcg atcagcgcct gcgcgtcgtc
      241 tcccgggtgc agtgcggcca caccgcccga gatcgtcacc gtgaacagca cctcctgcgt
      301 gctgcggttg cggatccgca tcgccttggc acgcagccgc acctgctcgg ccaggcgcgc
      361 cgcttcggtc agcgtgctgt gcggcagcac gatcgcgaac tcctcgccgc catagcgcgc
      421 cgtggcgtgt tcggggtcgg tcacggtcag tcgcaggatc tcgcccaggc cctggatcac
      481 gcggtcgccc atcacatggc cgtgcgcgtc gttgaccttc ttgaagtggt cgatgtcgag
      541 catcaccagg cagtgctgct ggcccgcggc gggcggccgc tgcagcaacg cggcgagtcg
      601 ctggtcgaag cccttgcggt tcaggatgct ggtcagcggg tcgagcagcg cctcgccgcg
      661 cgcccggtcg aggtcgctgc gcagccgcgc gatctcggtc tggctggcac tgacctgctg
      721 ctgcagcgcg gccgccgagc tcttcatctc ggcggtgccg gcgcgggcct ggtcgaggtg
      781 cggcgacagc gccgcggtgt cgctggagcg cagcgcggcc gacaggtcgt cgagctgcgc
      841 gccgaagcga tgcgcatccg tgccggtctg tgacgcgcgg ctggcgatgc cgctcatgac
      901 gcgctgcagc tcgccgccga tgcgctgcac cgtcgccgcg tcgggcggaa cgatgtggtc
      961 ctggtagagc tgcgcgacca ccgtgtcgct gaacggcacg ctgtcctgca ggcgctgctc
     1021 gaccgcctca tgcagggtcg ggttgatgcc ggccgcgtac tcgtaccaga gcgtgaaggt
     1081 gaccgggttg aacgccgcct cgtggcggcc catgtggccc agcgccacgc gcagcagctc
     1141 ggcgctgcgc tccttcggtt cggtgtaacg catcggttgg ttcgccctgg ctgtcgtcac
     1201 ggatggtgca tacgcgggaa gcgaccgtcg ccactcagaa gcgcgaaccc ggctcgcgca
     1261 ggaatgccac ttcttcgggc gtcgattgtc ggcccaatat cgcgttgcgg tgcgggaagc
     1321 gaccgaagcg ctcaaccact gcccgatgct tgtgggcgta agccaacatc ggctgcaact
     1381 cgggcgcctc ggccgccagg cgcgtgaaga gccgcaccgc ctcgtcctgc gcgcccaaac
     1441 cttcggcgtg ttcgaagggc aggtagacga acgcacgcca ctgcaccggc aaggcctcgt
     1501 catggcgcgc gccgaccatc gcggtggcgg cgcgcagcgc gcgcgcatcg ccggcgaagg
     1561 cgcgcggcgt gtcgcgaaag acgttgcggg tgaactggtc gagcagcagg atctgcgcga
     1621 gcgcggcgcg cggctgcgcc gcccagtcgg cgagctcgcc gcgcagcgcg cgctcgatca
     1681 acgcgccgaa gcgctgcgcg atctcgcgat cggtggcctc gctcttggtg aaccagaagt
     1741 cgcgctccgg gccgtcgtcg gcaccgaacc agtagtcgag cacggcctgg gcgtcggccg
     1801 gcagtgcgcc cgcatcggcg acagcggtgc caccgagccg gtgccacagg taggtcatct
     1861 cgagcgcctg cacgtgcgcg cgctgcgcgt tgtcggcggc gccgccgtga ccgccttcga
     1921 tgttctcgta gtacagcacc ggctgcccga gcgcgagcat ctttgccgcc atcttgcgcg
     1981 cgtggccggg gtggacacgg tcgtcgcggg tcgaggtggt gaacagcacc tcggggtagc
     2041 gccgcccggg ctgcaggttc tggtatgggc tgtagcgcgc gatccaggcc cattcctcgg
     2101 gcaggtccgg gttgccgtac tcggccatcc acgaggcgcc ggcgagcagc cggtggtagc
     2161 gccgcatgtc gagcagcggc acgctgcaca cgaccgcgcc gaacagctcc gggcgctgcg
     2221 tgaacgctgc gccgaccagc aacccgccgt tgctgccgcc ttcgatgccc aggcgcgccg
     2281 ggctggtgat gcggcgcgcg atcaggtcct gcgcgaccgc gatgaagtcg tcgtagctgc
     2341 gctgcttgtg cgccttcagc gcggcctggt gccaggccgg gccgtactcg ccgccgccgc
     2401 ggatgttcgc gaccaccagc acgccgccgc gcgccagcca gccgcggccg tagccggcgg
     2461 cgtaccaggg ctgcatcgag atctcgaagc cgccgtagcc gtagagcagg gtcggcgtgc
     2521 tgccgtcgcc gggccgcgcg gcgtcgacgc cggccggcca gaccacgaag tagggcacgc
     2581 gcgtgccgtc ggcgctggtc gcgaagaact ggtcgacgtg catgccgctc gcgtcgaagc
     2641 gcggcggcag cgccttcagc gtctcgcgcg catcgctgcc ggcgcgtgcg agccagagcg
     2701 cgtcgggcgt caggaaatcg ctgtagccga gcaagtagtg gtcggccagc gggtcgtcca
     2761 cgcgcgccgg gtcgtgcagg ccggcggccg tcagcgtgcc cgggaacggc gcgtcgatcg
     2821 cgcgccgcac gaagccctgc ggcgtgcgct gccactcttc gagccggctc gcgacgtcct
     2881 cgagcacgtt gaggacgaca tggtcgcgcg tcgtggtgtg gccggcca
//
LOCUS       DATMJB010000402         3598 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_679528, whole genome shotgun sequence.
ACCESSION   DATMJB010000402 DATMJB010000000
VERSION     DATMJB010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679528"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1394
                     /locus_tag="VNU48_08705"
     CDS             72..1394
                     /locus_tag="VNU48_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HWI11392.1"
                     /translation="MAANYLPYEPQQMLLLPEALQDWLPEGHLAHFISDAVDGLDLGA
                     FHVRYDKDGPRNQPFHPAMMVKVLVYGYATGCFSSRKIARKLHEDVAFRVLAARNFPA
                     HRTIRDFRAFHLKELSDLFVQVVRLAREMGLVKLGTIAVDGTKVKANASRHKAMSYGH
                     LLKAETELKAQITALLDKARAADEAEKNEPELDIPAEIARRQDRLDAIAAARARLEQR
                     QRDADLERGRSDDDDQKPRGHDGKPKGGRYKRPFGVPQDKDQDNFTDPDSRIMQRAGG
                     GFDPAYNAQTAVDATAHIIVAAELINNASDVRELPTMVEVVKDTLGAYPEQTLADAGY
                     RSEAVFEALAGCTDLVVAIGREGKGHRPINEATLPLTAAMVAKMKSQEARDAYRRRKW
                     LAEPPNGWIKNVLGFRQFSMRGLHRVQAEWKLVCMALNLRRMATMQPG"
     gene            1711..2145
                     /locus_tag="VNU48_08710"
     CDS             1711..2145
                     /locus_tag="VNU48_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020070878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWI11393.1"
                     /translation="MGDAKTYTGSCFCGAVGLTVSGEPAGMGYCHCESCRHWSAGPVN
                     AFTLWKPDAVRVTRGADNIGTYNKTPNSYRKWCKTCGGHLFTEHPGMGLTDVYAATIP
                     AFPYQAGVHLNYQETVLHLHDGLPKLKDIPKEMGGSGISVPE"
     gene            2207..>3598
                     /locus_tag="VNU48_08715"
     CDS             2207..>3598
                     /locus_tag="VNU48_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HWI11394.1"
                     /translation="MITEPLTRLFGPWVRQVDRLTLRADLIAGVLGALLVLPQGFAFA
                     TLAGLPPEYGLYTAIVPCVIAALFGSSWHVASGPTNANSLALFATLAPLAAVGSPAYI
                     QLALAVTVLVGLMQWLIGALHLGGLANFISPSALRGFMSGAAALIAVHSLTDLLGLPA
                     PETHATGALFVHIGAHLDALRPGALLVGAVTLVVVLVLRQVLPRWPTMLIGLAAGTAF
                     AALLNDVTPWPQWGAVAVVGSIPPIVPRFHWPGVALSSLPELLSIAFALTIVALGQSI
                     SIAKAVAARSGQAIDPNREFRGQGLSNIVGGFFSSYVSCGSLNRSMPNLEAGARTPLA
                     AVFASLWLLLLIAFTSRLLALIPLPAIAALLLVIAWSLFDIPGWRRLWAHSRQDFTIA
                     LVTALATMTIRIEMAILLGTILSLVTYLYRTSRPFMRVMGFDSTEHERPFVVRADVPA
                     PLPECPQLKMVRME"
BASE COUNT          563 a         1224 c         1217 g          594 t
ORIGIN      
        1 gcctaggagt ctgggcggca gaaggaccag tttgtgtcgg cgtggatgag gcgtagatcg
       61 ttgatcgagg catggccgcg aactacctcc cctacgaacc gcagcagatg ctgctgctgc
      121 ccgaagcgct tcaagactgg ctgcccgaag ggcacctggc gcacttcatc agcgacgccg
      181 tcgacggact cgaccttggc gcgttccacg tgcgctacga caaggacggc ccgcgcaacc
      241 agcccttcca cccggccatg atggtcaagg tgctggtcta cggctacgcc acggggtgtt
      301 ttagctcgcg caagatcgcg cgcaagctgc acgaggacgt ggcgttccgc gtgctggccg
      361 cgcgcaactt ccccgcgcac cgcacgatcc gcgacttccg ggcctttcac ctgaaggagc
      421 tctcagatct gttcgtgcag gtcgtgcgcc tggcccgcga gatgggactg gtcaagctgg
      481 gcacgatcgc ggtggacggc acgaaggtca aggccaacgc gagccgccac aaggccatga
      541 gctacgggca cctgctcaag gccgagaccg agttgaaggc gcagatcacg gccttgctcg
      601 acaaggcgcg tgcggccgac gaggccgaga agaacgagcc cgagctggac atcccggccg
      661 agatcgcgcg gcgccaggac cggctcgacg ccatagccgc agcgcgtgcc cgactcgaac
      721 aacgccaacg cgatgccgac ctcgaacgcg ggcgcagcga cgacgatgat cagaagccgc
      781 gcggccacga cggcaaaccc aagggcgggc gctataagcg gcccttcggc gtgccgcagg
      841 acaaggatca agacaacttc accgatcccg acagccgcat catgcagcgc gccggcggcg
      901 ggttcgaccc ggcgtacaac gctcagacgg cggtcgatgc cacagcccac atcatcgtgg
      961 ctgccgaact gatcaacaac gcctcggacg tgcgcgaatt gcccacgatg gtggaggtgg
     1021 tcaaggacac gcttggcgcc tatcccgagc agaccttggc cgatgcgggc taccgcagcg
     1081 aggcggtgtt cgaggcgctc gccggctgca cagacctggt cgtggccatc gggcgcgaag
     1141 gcaaggggca ccgcccgatc aacgaagcaa cgctgccgct cactgcggcg atggtggcga
     1201 agatgaagtc gcaggaagcg cgcgacgcct accgccgccg caaatggctg gccgagccac
     1261 ccaacggctg gatcaagaat gtcctgggct tccggcagtt cagcatgcgg ggcctgcacc
     1321 gggtgcaagc cgagtggaaa ctcgtttgca tggcgttaaa cctgcgacgg atggcgacga
     1381 tgcagccggg ttgaagggct gcaacagcat ttggaagaac ttcgcaggct tcgctatacc
     1441 gccatcgcga tctctgacag cgcccaaatc tcagccgccg gcgcaaactc cctcagccca
     1501 cctgcgttgc cttcctccat ccggcataaa agcgtctgcc gcccagactc ctagccctgc
     1561 ggcctgtggc ggcccggcgc gagccctccg atagcgctaa caggccctgg ccgctcccgg
     1621 tgtggacgtt tgcggccgta tcggagtagg ctgcaggctc gttctttttc tggcctttgt
     1681 cgttgttccc ttcacgaagg agattccgtc atgggagatg cgaagactta cacgggcagt
     1741 tgcttctgcg gtgccgtggg cctcacggtc agcggcgagc ccgccgggat gggctattgc
     1801 cactgcgaat catgccggca ctggtcggcc ggcccggtga atgcgttcac cttgtggaag
     1861 cccgacgccg tgcgggtcac gcgcggcgcg gacaacatcg gcacctacaa caagacgccg
     1921 aacagctatc gaaagtggtg caagacctgt ggcgggcacc tcttcaccga gcatcccggc
     1981 atggggctca cggatgtcta cgccgcgacc attccggcgt tcccctacca agccggcgtg
     2041 cacctgaact accaggaaac ggtgctgcac cttcacgacg gcttgccgaa gctgaaggat
     2101 attccgaagg agatgggcgg ctcgggcatc agcgtgcccg aatagcgggc agcgccccgc
     2161 gcgccgagcg gggcgccttg cgccaggcac cgcacaatgc gggctcatga ttaccgagcc
     2221 cctgacgcgt ttgtttggcc cctgggtgcg ccaggtcgat cggctgacct tgcgcgccga
     2281 cctgatcgcc ggcgtgctgg gggcgctgct ggtgctgccg cagggcttcg cgttcgcgac
     2341 gctcgccggc ctgccgccgg agtacggcct gtacaccgcg atcgtgccct gcgtgatcgc
     2401 ggcgctgttc ggctcgagct ggcacgtggc ctcggggccg acgaacgcga actcgctggc
     2461 gctgttcgcg acgctggcgc cgctcgccgc ggtcggctcg ccggcctaca tccagctcgc
     2521 gctggcggtc acggtgctgg tcgggctgat gcagtggctg atcggcgcgc tgcacctggg
     2581 cgggctggcc aacttcatct cgccgtcggc gctgcgcggc ttcatgagcg gcgcggcggc
     2641 gctgatcgcg gtgcattcgc tgaccgacct gctcggcctg ccggcgcccg aaacccacgc
     2701 caccggcgcg ctgttcgtgc acatcggcgc gcacctggac gcgctgcgcc ccggcgcgct
     2761 gctggtgggc gccgtcacgc tcgtggtcgt gctggtgctc aggcaggtgc tgccgcgttg
     2821 gccgaccatg ctgatcgggc tggcggcggg caccgcgttc gcggccttgc tgaacgacgt
     2881 cacgccgtgg ccgcaatggg gcgcggtcgc ggtcgtcggc agcatcccgc cgatcgtgcc
     2941 gcgctttcac tggcccggcg tcgcgctgtc cagcctgccc gagctgctgt cgatcgcgtt
     3001 cgcgttgacg atcgtcgcgc tcggccagtc gatctcgatc gcgaaggcgg tggccgcgcg
     3061 ctccggccag gcgatcgacc cgaaccgcga gttccgcggc cagggcttgt cgaacatcgt
     3121 cggcggcttc ttctcgtcgt acgtgtcctg cggctcgctg aaccgctcga tgcccaacct
     3181 cgaggccggc gcgcgcacgc cgctggctgc ggtgttcgcg tcgctgtggc tgctgctgct
     3241 gatcgcgttc acgtcgaggc tgctggcgtt gatcccgctg ccggcgattg ccgcgctgct
     3301 gctcgtgatc gcatggtcgc tgttcgacat tccgggctgg cggcgcctgt gggcgcacag
     3361 ccggcaggac ttcacgatcg ccctcgtcac ggcgctggcg acgatgacga tccgcatcga
     3421 gatggcgatc ctgctcggca cgatcctgtc gctggtgacc tacctgtacc gcacctcgcg
     3481 cccgttcatg cgcgtgatgg gcttcgacag caccgagcac gagcgcccgt tcgtcgtgcg
     3541 cgccgacgtg ccggcgccgc tgcccgagtg cccgcagctg aagatggtgc ggatggaa
//
LOCUS       DATMJB010000403         2551 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_432834, whole genome shotgun sequence.
ACCESSION   DATMJB010000403 DATMJB010000000
VERSION     DATMJB010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432834"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VNU48_08720"
     CDS             <1..694
                     /locus_tag="VNU48_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HWI11395.1"
                     /translation="ARMYLRHCERRGWKSELMSESVSDLGGYKEVVIRIEGEDVYARL
                     KFESGGHRVQRVPVTETQGRIHTSACTVAVLPEPDEAEEVTLNPAELRIDTFRASGAG
                     GQHVNKTDSAIRITHLPTGIVAECQDDRSQHRNKAKAMAVLTARLRDKDRNERAAKEA
                     ATRKGLIGSGDRSDRIRTYNFPQGRFTDHRINLTLYKLQAILDGDLIEVTEALQAAQA
                     AEQLAALEAGEA"
     gene            691..1521
                     /gene="prmC"
                     /locus_tag="VNU48_08725"
     CDS             691..1521
                     /gene="prmC"
                     /locus_tag="VNU48_08725"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWI11396.1"
                     /translation="MSDAATTVAAALRDAKAQGLDRLDAQLLLAHVLARPRSWLLAHD
                     DAALDDPQRGAWQALLARRLTGEPLAYLVGAKEFHGLALQVDANVLVPRPETELLVDW
                     AIERLMAMSSPDPRVVDLGTGSGAIALAVKHAVPPAQVLATDVDPAALQVAQTNARRL
                     GLDIGWRRGSWWSCLAGERFGLILSNPPYIAGGDPHLAALRHEPALALTPGGDGLDAL
                     RAIVAGAADHLGPGGWLLLEHGHDQADAVQALLRAHDFVAIETRRDLGGRPRCTGARR
                     "
     gene            1675..2292
                     /locus_tag="VNU48_08730"
     CDS             1675..2292
                     /locus_tag="VNU48_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11397.1"
                     /translation="MRNSAWMLAVVLTFPSVPALAADGDGAMPGSGQLPWARWQGRLS
                     LGTTSSPWRPGADTAAPTLSSASLMGDYYFSRWLTGPQGLGGFRATSGLIFGQRSMLS
                     TGQPGFATGGAFSIGSRSTARAPAPYPGDAAGDSTTLPYVGLGYTGLSPRSGWSFSAD
                     LGLVAQGAGGAARLGRSLYGSQSLDDAVRDLRMTPLVQLGVTYAF"
     gene            2394..>2551
                     /locus_tag="VNU48_08735"
     CDS             2394..>2551
                     /locus_tag="VNU48_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HWI11398.1"
                     /translation="MSDVQQRIADIVKSHRVVLFMKGTAQFPQCGFSGRAIQVLKACG
                     ATDITTVNV"
BASE COUNT          376 a          901 c          922 g          352 t
ORIGIN      
        1 ggcgcgcatg tacctgcgcc actgcgagcg ccgcggctgg aagtccgagc tgatgagcga
       61 gagcgtcagc gacctgggcg gctacaagga ggtggtgatc cgcatcgagg gcgaggacgt
      121 ctacgcgcgg ctcaagttcg agtccggcgg gcaccgcgtg caacgcgtgc cggtgaccga
      181 gacgcagggc cgcatccaca ccagcgcctg cacggtcgcg gtgctgcccg agcccgacga
      241 ggccgaagag gtcacgctga accccgccga gctgcgcatc gacaccttcc gcgccagcgg
      301 cgccggcggc cagcacgtca acaagaccga cagcgcgatc cgcatcacgc acctgccgac
      361 cggcatcgtt gccgagtgcc aggacgaccg ctcgcagcac cgcaacaagg ccaaggcgat
      421 ggccgtgctc accgcgcggc tgcgcgacaa ggaccgcaac gagcgcgccg cgaaggaagc
      481 tgccacccgc aagggcctga tcggcagcgg cgaccgcagc gaccgcatcc gcacctacaa
      541 ctttccgcag gggcggttca ccgatcaccg catcaacctg accttgtaca agctgcaggc
      601 gatcctggac ggcgacctga tcgaggtgac cgaggcgctg caggccgcgc aggcggccga
      661 gcagctggcg gcgctcgagg ccggcgaagc gtgagcgacg cggcgacgac cgtggccgct
      721 gcgctgcgcg acgcgaaggc gcagggcctg gatcggctcg acgcgcaact gctgctcgcc
      781 cacgtgctgg cgcgcccgcg cagctggctg ctggcccacg acgatgcagc gctcgacgat
      841 ccgcagcgcg gtgcctggca ggcgctgctg gcgcgccggc tgaccggcga gccgctcgcg
      901 tacctggtcg gcgcgaagga gttccacggg ctggcgctcc aggtcgacgc gaacgtgctg
      961 gtgccgcggc ccgagaccga gctgctggtg gactgggcga tcgagcggct gatggcgatg
     1021 tcgagccccg atccgcgcgt cgtcgacctg ggcaccggca gcggggcgat tgcactggcc
     1081 gtcaagcacg cggttccgcc ggcgcaggta ctggccaccg acgttgaccc cgcggcgctg
     1141 caggtcgcgc agacgaacgc caggcgcctc ggtctggaca tcgggtggcg ccggggcagc
     1201 tggtggagtt gcctggccgg cgagcgcttt ggcctgatcc tcagcaaccc gccctacatc
     1261 gccggcggcg acccgcacct cgccgcgctg cgccacgagc cggcgctcgc gctgacacct
     1321 ggaggcgacg ggctggatgc gttgcgcgcg atcgtcgccg gcgccgccga ccatctcggg
     1381 ccgggcggct ggctgctgct ggagcacggc cacgaccagg ccgatgccgt ccaggcgctg
     1441 ttgcgggcgc acgacttcgt ggccatcgag acgcggcgcg acctgggcgg tcggccacgc
     1501 tgcaccggtg cccggcgttg atggccggca tcgcgtgacg gacgccacat cgggccgtcg
     1561 atccgggggg tgacattggc aacacgggcc gcattcaacg gcgtacgatg aagtcgcagt
     1621 gcggtggtcg gatggcgccg cggtgccgga cccggtcgtc gaaaggaggc tgcaatgcgc
     1681 aattcagcgt ggatgctcgc ggtggtgttg accttcccct cggtgcccgc tctggccgcc
     1741 gacggtgatg gcgcgatgcc gggttccggg caacttccgt gggcgcgctg gcaaggccgt
     1801 ctgtcgctgg gcaccacgtc gtcgccctgg cgccccgggg ccgacactgc tgcacccacg
     1861 ctcagcagcg ccagcctgat gggcgactac tacttcagtc gctggctgac cggcccgcag
     1921 ggcctgggcg ggttccgcgc caccagcggg ctgatcttcg gccagaggtc gatgctgagc
     1981 accggtcaac cgggtttcgc gaccggcggc gcgttcagca tcggttcacg ctcgaccgcc
     2041 cgcgcgccag cgccctaccc cggcgatgcg gcgggcgaca gcaccacctt gccttatgtc
     2101 ggcctgggct acaccggcct gtcgccgcgc agcggctgga gcttcagtgc cgacctcggc
     2161 ctggtggcgc aaggcgccgg cggggccgcg cgcctcgggc gcagcctgta cggcagccag
     2221 agcctggacg acgcggtgcg cgacctgcgg atgacgccgc tggtgcaact cggcgtgacg
     2281 tacgcgttct gagtccgggc cgtgcccggg gccttgtggg cggcggcgcc agagactttg
     2341 cggtttgccg tataacctcc actatccccc agctttctcg cgagccagcc gacatgagtg
     2401 acgttcaaca acgcatcgcc gacatcgtca agagccaccg cgtggtgctt ttcatgaagg
     2461 gcacggcgca gtttccgcag tgcggcttct cgggccgcgc gatccaggtg ctgaaggcct
     2521 gcggtgcgac cgacatcacc accgtcaacg t
//
LOCUS       DATMJB010000404         3104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_157604, whole genome shotgun sequence.
ACCESSION   DATMJB010000404 DATMJB010000000
VERSION     DATMJB010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157604"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2311)
                     /gene="clpB"
                     /locus_tag="VNU48_08740"
     CDS             complement(<1..2311)
                     /gene="clpB"
                     /locus_tag="VNU48_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HWI11399.1"
                     /translation="MRIDKLTSKFQEALADAQSLAVTNDNPYIEPAHLLAAMLAQADG
                     PKALLDRAGANSAALKTALDTAIKGLPQVQGSGQVVQPGRDLAALLQAAEKEAGKRGD
                     QFIASEMFLLALADSKTDLGGIARGHGLNRKALEAAIEAVRGGQKVDSADAEGQREAL
                     KKYTLDLTERARKGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVAGEVPDSLKNKRVLVLDMALLLAGAKFRGEFEERLKGVLKEVAQDEGQTIVFI
                     DELHTMVGAGKAEGAIDAGNMLKPALARGELHCIGATTLDEYRKYIEKDAALERRFQK
                     ILVGEPTVEATIAILRGLQQKYEVHHGVEITDPAIVAAAELSQRYITDRFLPDKAIDL
                     IDEAASKVRIEIDSKPEAMDKLDRRLIQLQIERAAVTREKDEASQRRLGLIEEEINRV
                     QREAADLDEIWKAEKAAAQGSAHLMEEIDRVKFQIEEWKRKGDFNKVAELQYGQLPTL
                     EKTLKDAQAKEAKKGASGKGKGKPQLLRTTVGAEEIAEVVARATGIPVSKLMQGDREK
                     LLQMEAKLHERVIGQDEAITAVADAIRRSRSGLSDPNRPTGSFLFLGPTGVGKTELCK
                     ALAGFLFDSEDHLIRLDMSEFMEKHSVARMIGAPPGYVGYDEGGYLTEAVRRKPYCVL
                     LLDEVEKAHPDVFNVLLQVLDDGRMTDGQGRTVDFKNTVIVMTSNLGAQYLVALGEHE
                     DVDAVRDQVMTEVRLAFRPEFLNRVDEIIL"
     gene            complement(2390..2779)
                     /gene="crcB"
                     /locus_tag="VNU48_08745"
     CDS             complement(2390..2779)
                     /gene="crcB"
                     /locus_tag="VNU48_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HWI11400.1"
                     /translation="MIGAWPWVQVLAVAIGAAVGGLARWLAGLWLNAQWAGFPLGTLL
                     VNCVGGLLIGIALCWFERTPNELLRLLLVTGFLGGLTTFSSFSGESLVLLHRGQFVLA
                     LGHTAAHLFGSLACAALGYAALNALLD"
     gene            complement(2776..>3104)
                     /locus_tag="VNU48_08750"
     CDS             complement(2776..>3104)
                     /locus_tag="VNU48_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HWI11401.1"
                     /translation="GMTERAVEAMIDAALQRFDILGARVIHRVGLLQPLDQIMMVAVT
                     GAHRGQAFQACEFLMDYLKTQAPFWKKEQTPEGARWVDARSSDDAAIARWGISAGNAP
                     PEPPAR"
BASE COUNT          418 a         1093 c         1031 g          562 t
ORIGIN      
        1 acaggatgat ctcgtcgacc cggttgagga actcgggccg gaacgccagc ctgacctccg
       61 tcatcacctg gtcgcgcacc gcgtcgacgt cctcgtgctc gccgagcgcc accaggtact
      121 gggcgccgag gttcgaggtc atcacgatca cggtgttctt gaagtccacg gtgcggccct
      181 ggccgtcggt catgcggccg tcgtcgagca cctgcagcag cacgttgaac acgtccggat
      241 gcgccttctc gacctcgtcg agcagcagca cgcagtaggg cttgcgccgc accgcctcgg
      301 tcaggtagcc gccctcgtcg tagccgacgt agcccggcgg cgcgccgatc atgcgggcga
      361 ccgaatgctt ctccatgaac tcgctcatgt cgaggcggat caggtggtcc tcgctgtcga
      421 acaggaagcc ggccagggcc ttgcacagct cggtcttgcc gacgccggtg gggccgagga
      481 acaggaacga gccggtcggg cggttcgggt ccgacaggcc cgagcgtgaa cggcggatcg
      541 cgtcggcgac cgcggtgatc gcctcatcct ggccgatcac gcgctcgtgc agcttggctt
      601 ccatctgcag cagtttctcg cggtcgccct gcatcagctt ggacaccggg atgccggtgg
      661 cgcgcgccac gacctcggcg atctcctcgg cgccgacggt cgtgcgcagc agctgcggct
      721 tgcctttgcc cttgccactt gcgcccttct tcgcctcctt ggcctgcgca tctttcagcg
      781 tcttttccag cgtcggcagc tggccgtact gcagctccgc gaccttgttg aagtcgccct
      841 tgcgcttcca ttcctcgatc tggaacttga cccggtcgat ctcttccatc aggtgcgccg
      901 agccttgcgc cgccgccttc tcggccttcc agatctcgtc gaggtcggca gcttcgcgct
      961 gcacgcggtt gatctcttcc tcgatcaggc cgaggcggcg ttgcgaggct tcgtcctttt
     1021 cgcgcgtgac ggcggcgcgc tcgatctgca gctggatcag gcgccggtcg agcttgtcca
     1081 tcgcctcggg cttggagtcg atctcgatgc gcaccttgga cgccgcctcg tcgatcaggt
     1141 cgatcgcctt gtcgggcagg aagcggtcgg tgatgtagcg ctggctcagc tcggccgcgg
     1201 cgacgatcgc cgggtcggtg atctcgacgc cgtggtgcac ctcgtacttc tgctgcagcc
     1261 cgcgcaggat cgcgatcgtc gcctccacgg tcggctcgcc gacgaggatc ttctggaagc
     1321 gccgctccag cgccgcgtcc ttctcgatgt acttgcggta ctcgtcgagc gtggtcgcgc
     1381 cgatgcagtg cagttcgccg cgcgccagtg cgggcttgag catgttgccg gcgtcgatcg
     1441 cgccctcggc cttgccggcg cccaccatcg tgtggagttc gtcgatgaag acaatggtct
     1501 ggccttcgtc ctgcgccact tccttcagca cgcccttgag ccgttcctcg aactcgccgc
     1561 ggaacttggc gccggccagc agcagcgcca tgtcgagcac gagcacgcgt ttgttcttca
     1621 gcgaatcggg cacctcgccc gcgacgatgc gctgcgccag gccctcgacg atcgcggtct
     1681 tgcccacgcc cggctcgccg atcagcaccg ggttgttctt ggtgcggcgt tgcagcacct
     1741 ggatcgctcg gcggatctcg tcgtcgcgcc cgatcaccgg gtcgagcttg cccttgcggg
     1801 cgcgctcggt caggtcgagc gtgtatttct tcagcgcctc gcgctggccc tcggcgtcgg
     1861 cgctgtcgac tttctggccg ccgcgcaccg cctcgatcgc cgcctccagc gccttgcggt
     1921 tcaggccatg gccgcgcgcg atgccgccca ggtcggtctt cgagtcggcg agcgcgagca
     1981 ggaacatctc gctggcgatg aactggtcgc cgcgcttgcc ggcctctttc tcggccgcct
     2041 gcagcagcgc cgcgagatcg cggcccggct gcaccacctg gccgctgccc tggacctgcg
     2101 gcaggccctt gatggcggtg tcgagcgcgg tcttcagcgc ggcactgttg gcgccggcgc
     2161 gatcgagcag cgccttcggg ccgtcggcct gcgccagcat cgcggccagc agatgcgccg
     2221 gctcgatgta agggttgtcg ttcgtgacgg ccaggctctg cgcgtcggcg agcgcttcct
     2281 ggaacttgct ggtgagtttg tcgattcgca tggggatgat cctccgattg cgtcggacat
     2341 gaggtggatg atctccattt tcaagccgcc ggcgcggccc ggcttgcgct caatcaagca
     2401 aggcgttcag cgccgcatag cccagcgccg cgcaggccag cgacccgaac agatgcgcgg
     2461 cggtgtggcc gagtgccagc acgaactggc cgcgatgcag cagcacgagg gactcgcccg
     2521 agaacgacga gaaggtcgtc agcccgccga ggaagccggt caccagcagc aaccgcagca
     2581 gttcgttcgg cgtgcgttcg aaccagcaca gcgcaatgcc gatcagcagg ccgccgacgc
     2641 agttgacgag cagtgtgccg agcggaaagc cggcccactg cgcgttcagc cagagcccgg
     2701 cgagccagcg cgccagcccg ccgacggccg cgccgatcgc gacggcgagc acctgcaccc
     2761 agggccaggc gccgatcatc gggcaggcgg ctcgggcggc gcgttgcctg cgctgatgcc
     2821 ccagcgcgcg atcgccgcgt cgtcgctgct gcgcgcatcg acccagcgcg cgccctcggg
     2881 cgtctgttcc ttcttccaga acggcgcctg ggtcttcagg tagtccatca ggaactcgca
     2941 ggcctggaag gcctggccgc ggtgcgcgcc ggtgaccgca accatcatga tctggtccag
     3001 cggctgcaac aggccgacgc ggtggatcac gcgcgcgccg aggatgtcga agcgctgcag
     3061 cgccgcatcg atcatcgcct cgaccgcacg ctcggtcatg ccgg
//
LOCUS       DATMJB010000405         2062 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_419242, whole genome shotgun sequence.
ACCESSION   DATMJB010000405 DATMJB010000000
VERSION     DATMJB010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419242"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VNU48_08755"
     CDS             <1..425
                     /locus_tag="VNU48_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI11402.1"
                     /translation="TAAKPKIDATLATRHPLRILLAEDNVVNQKLALRLLQRMGYRAD
                     LASNGIEAIESIERQTYDVVLMDVQMPEMDGLEASRRIRARWPAGERPRIVAMTANAM
                     QGDREMCLDAGMDDYLTKPIRVDQLVEALNGASARKDP"
     gene            427..774
                     /locus_tag="VNU48_08760"
     CDS             427..774
                     /locus_tag="VNU48_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWI11403.1"
                     /translation="MSDKMSNPAIDAAAFAELEASAGAEFARELIGTFLEEAPGMLAE
                     LRAAFAAGQADGFRRSAHSLKSNSLTFGALALAAQARALELQGMPADATALDRLEAEY
                     ARTAAALTELRHG"
     gene            767..1816
                     /locus_tag="VNU48_08765"
     CDS             767..1816
                     /locus_tag="VNU48_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWI11404.1"
                     /translation="MADLRNAPARLLVADDNKVNRLLLTRSLELQGHRVACAENGRVA
                     LEMLRGEVFDLLLLDIEMPEMDGFQVLEQLFADRRLRDLPVIVTSSLEGIANLVRCIE
                     LGAEDYLGKPVNPVLLRARIGASLEKKRLRDQQKELVRRFATPEVAQDLQDSGFALGG
                     RRVTGSVMFSDIRGFTALVESQPPEETIELLNTYYTLMFDAISGHGGVVNQMVGDGLM
                     AIFGAPLPLPDHAGAAVRAALDMIEMIELLNAERASEGRRALRIGIGIASGEMVAGYT
                     GTQARATYTCIGDTVNLAARLEAHTKIAQRAILIDSATRAALGEQVACDALGTALFHG
                     KAAPVEVFAVGAASV"
BASE COUNT          335 a          708 c          722 g          297 t
ORIGIN      
        1 ccactgctgc aaagccgaag atcgatgcta cgctggcgac gcgccacccg ttgcgcatcc
       61 tgctcgccga ggacaacgtg gtgaatcaga aactggcctt gcggctgctg cagcggatgg
      121 gctaccgcgc cgacctggcg agcaacggca tcgaggcgat cgagagcatc gaacgacaga
      181 cctacgacgt ggtgctgatg gacgtgcaga tgcccgagat ggacggcctc gaagcgtcgc
      241 gccgcatccg cgcacgctgg cctgccggcg agcgcccgcg cattgtcgcg atgaccgcca
      301 acgcgatgca gggcgatcgc gagatgtgtc tggacgcggg catggacgac tacctgacca
      361 agccgatccg cgtcgaccaa ctggtcgagg cgctgaacgg cgcgtcggca cgcaaggacc
      421 catgagatga gcgacaagat gagcaaccct gcgatcgacg ccgcggcctt cgcggaactc
      481 gaggcctcgg ccggcgccga gttcgcgcgc gagctgatcg gcacatttct cgaagaggca
      541 ccgggcatgc tggccgagtt gcgcgcggcg ttcgcggcgg gccaggccga cggcttccgg
      601 cgctcggccc attcgctgaa gtcgaacagc ctcaccttcg gggcgctcgc gctggcggcg
      661 caggcgcgcg cgctggaact gcagggcatg ccggcggacg ccaccgcgct cgaccgcctc
      721 gaggccgagt acgcgcgcac cgcggcggcc ctgacggaac tgcgccatgg ctgacctgcg
      781 caacgccccg gcccggctgc tggtggccga cgacaacaag gtcaaccgcc tgctgctgac
      841 gcgcagcctc gagctgcagg ggcatcgcgt ggcctgtgcc gagaacggcc gtgtcgcgct
      901 cgagatgctg cgcggcgagg tcttcgacct gctgctgctg gacatcgaga tgcccgagat
      961 ggacggcttc caggtcctcg agcagctctt cgccgaccgg cgcctgcgcg acctgccggt
     1021 gatcgtgacc tcgtcgctcg aaggcatcgc caacctggtg cgctgcatcg agctcggcgc
     1081 cgaagactac ctgggcaagc cggtgaatcc ggtgctcttg cgcgcgcgca tcggcgcgag
     1141 cctggagaag aagcgcctgc gcgaccagca gaaggaactg gtgcgccgct tcgccacgcc
     1201 cgaggtcgcg caggacctgc aggactcggg cttcgcgctc ggcggccggc gcgtcaccgg
     1261 cagcgtgatg ttctcggaca tccgcggctt caccgcgctg gtcgagtcgc agccgcccga
     1321 agagacgatc gagctgctca acacctacta cacgctgatg ttcgatgcga tcagcggtca
     1381 cggcggcgtc gtcaaccaga tggtcggcga cgggctgatg gcgatcttcg gcgcgccgct
     1441 gccgctgccc gaccatgccg gcgccgcggt gcgcgcggcg ctcgacatga tcgagatgat
     1501 cgagctgctc aatgccgagc gcgcgagcga gggcagacgc gcgctgcgca tcggcatcgg
     1561 catcgccagc ggcgagatgg tggccggcta taccggcacg caagcgcgcg ccacctacac
     1621 ctgcatcggc gacaccgtga acctcgcggc acgcctcgag gcccacacca agattgcgca
     1681 gcgcgcgatc ctgatcgaca gcgcgacgcg cgcggccctg ggcgagcagg tcgcctgcga
     1741 cgcgctcggc acggccctgt tccatggcaa ggccgcgccg gtcgaggtgt tcgcggtcgg
     1801 cgcggcgagc gtctgagtcg ccttgatgac agcacttccg atcacgctgc gggcagcgtg
     1861 agtcagagca acccgcgccg gtagaggggc cttcgcatga cgatcacggc cgacatccga
     1921 acgaagctgc gcgccctcaa cgcgaggttc gaccggtggc cgaattggct tcaggcttgc
     1981 gtcactctct tcctttggct gctgctgttc acggtcggcc tcagggcatg cgcgggcacg
     2041 tcggccgacg ggctggaggg gc
//
LOCUS       DATMJB010000406         2244 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_182580, whole genome shotgun sequence.
ACCESSION   DATMJB010000406 DATMJB010000000
VERSION     DATMJB010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182580"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VNU48_08770"
     CDS             complement(<1..279)
                     /locus_tag="VNU48_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWI11405.1"
                     /translation="MAFGRLERSNRPTPMSDINMTPLIDVMLVLLVIFMITAPLMSSS
                     LKLDLPRSDAGQPNDAPQSIAVALDKDGRTFFGDEALEPAAFAARVAAA"
     gene            complement(290..952)
                     /locus_tag="VNU48_08775"
     CDS             complement(290..952)
                     /locus_tag="VNU48_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWI11406.1"
                     /translation="MSTSGLVNFWGQGDAVTRSVAALLLLMSISAWVVIFWKGWLLRR
                     VRGDIERAVPAFWAAASLEGGRDALAAFDRERALAPLLAAAMAAAPDGTLSAKGHADS
                     QLTRRLRDALHGVLDQLQFGQVLLASIGSTAPFIGLFGTVWGIYHALMGISVAGSMSI
                     ERVAGPVGEALVMTAAGLAVAIPAVLAYNVFGKRVAGCEAELEGFAHDLREMVLDAAG
                     AR"
     gene            complement(949..1767)
                     /gene="dapB"
                     /locus_tag="VNU48_08780"
     CDS             complement(949..1767)
                     /gene="dapB"
                     /locus_tag="VNU48_08780"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HWI11407.1"
                     /translation="MSGTAAPIRVAVAGASGRMGRMLIEAIGGADDMRLAGALDVAGS
                     PALGQDAAAFLGRASGAVITADLRGGLADADVLIDFTRPEGTLAHLAVCRELGVKAVI
                     GTTGFSDAQKAQIAEHAQHIAVMMAPNMSLGVNVVLKLLDVAARALSEGYDIEIVEAH
                     HRHKVDAPSGTALKMGEVVAAALGRDLKACAVYGREGVTGERDPSTIGFATVRGGDIV
                     GDHTVLFAGTGERIEITHRSNSRATYAQGSLRAARFLAGHERGLFGMDDVLGIR"
     gene            complement(1764..2240)
                     /locus_tag="VNU48_08785"
     CDS             complement(1764..2240)
                     /locus_tag="VNU48_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HWI11408.1"
                     /translation="MPTPNWSAERFLGVVTPYRVEVVQGNVITSEQAQAVKPGMNRAQ
                     VRDVLGTALVTDVFHADRWDYVFTIRRQGAEPQARRVVVRFDGDVLKSIDTGGALPTE
                     REFVASIDTFKTSRNAPPLELTPEQLKALPPPVKPPVVEVEPVGPTRTYPPLEPAT"
BASE COUNT          313 a          803 c          815 g          313 t
ORIGIN      
        1 cgcggcggcg acgcgcgcgg cgaacgccgc cggctcgagc gcctcgtcgc cgaagaaggt
       61 gcggccgtcc ttgtcgaggg cgacggcgat cgactgcggc gcgtcgttcg gctgccccgc
      121 gtcgctgcgc ggcaggtcga gcttgaggct cgagctcatc agcggcgcgg tgatcatgaa
      181 gatcaccagc agcaccagca tcacgtcgat cagcggcgtc atgttgatgt cgctcatcgg
      241 cgtcggccgg ttgctgcgct ccaggcgtcc gaaggccatg cggcgagcct cagcgcgcgc
      301 cggcggcgtc gagcaccatc tcgcgcaggt cgtgcgcgaa gccttcgagc tcggcctcgc
      361 agccggcgac cctcttgccg aagacgttgt aggccagcac cgcgggaata gcgaccgcca
      421 ggcccgcggc ggtcatcacc agcgcctcgc cgaccggccc ggcaacgcgc tcgatcgaca
      481 tcgagccggc caccgagatg cccatcagcg catggtagat gccccagacc gtgccgaaca
      541 ggccgatgaa cggcgcggtg ctgccgatcg aggcgagcag cacctggccg aattgcagct
      601 ggtcgagcac gccgtgcagc gcgtcgcgca ggcgccgcgt gagctgcgaa tcggcgtggc
      661 ccttggcgct gagcgtgccg tcgggcgccg cggccatcgc ggcggcgagc agcggcgcga
      721 gtgcgcgctc gcggtcgaac gcggcgagcg cgtcgcgacc gccttcgagc gaggccgcgg
      781 cccagaacgc cggcaccgcg cgttcgatgt cgccgcgcac gcgccgcagc agccagccct
      841 tccagaagat cacgacccag gcgctgatcg acatcagcag cagcagcgcc gcgacgctgc
      901 gcgtcactgc gtcgccctga ccccagaagt tgacgaggcc gctcgtgctc atcggatgcc
      961 gagcacgtcg tccatgccga acagcccgcg ctcgtgcccc gccaggaagc gcgcggcacg
     1021 caggctgccc tgcgcatagg tcgcgcggct gttggaacgg tgcgtgatct cgatgcgctc
     1081 gccggtgccg gcgaacagca ccgtgtggtc gccgacgatg tcgccgccgc gcaccgtcgc
     1141 gaagccgatg gtcgacgggt cgcgctcgcc ggtcacgcct tcgcggccgt agaccgcaca
     1201 ggccttcagg tcgcggccga gcgcggcggc caccacctcg cccatcttca gcgcggtgcc
     1261 gctgggggcg tcgaccttgt ggcggtgatg ggcctcgacg atctcgatgt cgtagccctc
     1321 gctcagcgcg cgcgccgcga catcgagcag cttcagcacg acgttgacgc ccaggctcat
     1381 gttcggcgcc atcatcacgg cgatgtgctg cgcgtgttcg gcgatctgcg ccttctgcgc
     1441 gtcgctgaag ccggtcgtgc cgatcaccgc cttcacgccg agctcgcggc agaccgcgag
     1501 gtgggcgagc gtgccttcgg gccgggtgaa gtcgatcagc acgtcggcat cggcgaggcc
     1561 gccgcgcaga tcggccgtga tcacggcgcc gctggcgcgg cccaggaacg ccgcggcgtc
     1621 ctggcccagc gccgggctgc cggcgacatc gagagcgccg gccagccgca tgtcgtcggc
     1681 gccgccgatc gcctcgatca gcatgcgccc catccggccc gacgcaccgg ccaccgcgac
     1741 gcggatcggg gctgccgtgc cgctcatgtg gccggttcga gcggcgggta ggtgcgcgtc
     1801 gggcccacgg gctcgacttc gacgacgggc ggcttgaccg gtggcggcag ggccttcagc
     1861 tgctcgggcg tcagctccag cggcggcgcg ttgcgcgagg tcttgaaggt gtcgatcgac
     1921 gcgacgaatt cgcgttcggt gggcagcgcg ccaccggtgt cgatgctctt gagcacatcg
     1981 ccgtcgaagc gcacgacgac gcggcgggct tgcggctcgg cgccctggcg gcggatcgtg
     2041 aagacgtagt cccagcggtc ggcgtggaac acgtcggtga ccagcgcggt gccaaggacg
     2101 tcgcgcacct gggcgcggtt catgccgggc ttgacggcct gcgcctgctc gctggtgatg
     2161 acgttgccct gcaccacctc gacccggtac ggcgtgacga cgccgagaaa gcgctcggca
     2221 gaccagttcg gcgtcggcag cgag
//
LOCUS       DATMJB010000407         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_36088, whole genome shotgun sequence.
ACCESSION   DATMJB010000407 DATMJB010000000
VERSION     DATMJB010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36088"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VNU48_08790"
     CDS             <1..204
                     /locus_tag="VNU48_08790"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWI11409.1"
                     /translation="PNRIIMPLFPPIPMKAKVFVAVFGGLELLFGVTGTMQGVAHFAH
                     LGGMLGAFLMIRYWQGRTPFGPR"
     gene            complement(293..1186)
                     /locus_tag="VNU48_08795"
     CDS             complement(293..1186)
                     /locus_tag="VNU48_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWI11410.1"
                     /translation="MTARAARLRRPTLLIVGCGDIGLRVAKLLRGRWRLLALTSSVER
                     CDVLRAAGVQPLLGDLDRPHTLARLAGLADAVLHLAPPPTSGATDPRTTRLLQALARK
                     GRARRIVYASTSGVYGDCGGARFDETRAVRPATDRARRRVDAEQRLRWYGRAFGARVT
                     LLRIPGIYAANRAGGHPRERLARGAPVLAPEHDVFTNHIHADDLARACVAALHRGSPQ
                     RVVHASDDTVLMMGDYFDLAADIEGVPRPRRVTIEQARTELSPMQLSFMGESRRLINT
                     RLKRELRLRLRYPTVAHGLRA"
     gene            1257..>2079
                     /locus_tag="VNU48_08800"
     CDS             1257..>2079
                     /locus_tag="VNU48_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HWI11411.1"
                     /translation="MPFTVTVQPSGRSFSVDRDEPILSAAIRQGIGLPYGCKDGACGS
                     CKCRLVEGRVILGVHQSKALSADEEAAGWILTCQAAPQTDVVIEARTVPGAGEFAIRK
                     MPTRVTTITKPAPDVAILQMQLPANDRLQYHAGQYIEFILRDGARRSYSMANAPHTQA
                     DKPGIELHLRHLPGGKFTDHVFTAMKEKDILRMEGPFGSFFLREDSDKPMILLASGTG
                     FAPIKAIIEHLIEKGSQRKAVLYWGCRSKADLYMHDWAEQATAKLAHLRYVPVLSE"
BASE COUNT          317 a          739 c          731 g          292 t
ORIGIN      
        1 ccgaaccgca tcatcatgcc gctgttcccg ccgatcccga tgaaggccaa ggttttcgtc
       61 gccgtgttcg gcggcctcga actgctgttc ggcgtgaccg gcacgatgca gggcgtggcc
      121 cacttcgcgc atctgggcgg catgctcggc gcgttcctga tgatccgcta ctggcagggc
      181 cgcacaccgt tcggtccgcg ctgaacgggt cagtagccgc gcacccggtc caccgtctgt
      241 ggtggcaact cgccacgctg cacgcagcgc aagtgtcttc acgatgtttt gctcaggcgc
      301 gcaggccgtg cgcgacggtc gggtagcgca ggcgcaggcg cagctcgcgt ttcaggcggg
      361 tgttgatcag gcgccgcgat tcgcccatga agctcagctg catcggcgag agttcggtgc
      421 gcgcctgctc gatcgtgacg cggcgcggac gcggcacgcc ttcgatgtcg gcggcgaggt
      481 cgaagtagtc gcccatcatc agcaccgtgt cgtcgctggc gtggaccacg cgctgcggcg
      541 agccgcgatg cagcgcggcg acgcaggcgc gtgccaggtc gtcggcgtgg atgtggttcg
      601 tgaacacgtc gtgctctggc gcaagcaccg gcgcgccgcg cgccaggcgc tcgcgcgggt
      661 ggccgccggc gcggttcgcc gcatagatgc cggggatgcg cagcagcgtc acgcgcgcgc
      721 cgaacgcgcg cccgtaccag cgcagccgct gctcggcatc gacgcggcgc cgcgcccgat
      781 cggtggcggg gcgaaccgcg cgcgtctcgt cgaagcgcgc accgccgcag tcgccgtaca
      841 cgccgctggt gctggcgtag acgatgcgcc gcgcccggcc cttgcgtgcc agcgcctgca
      901 gcagccgcgt ggtgcgcggg tcggtcgcac cgctggtcgg cggcggtgcg aggtgcagca
      961 ccgcatcggc caggccggcc aggcgcgcca gcgtgtgcgg ccgatcgagg tcgccgagca
     1021 gcggctgcac gccggccgcg cgcagcacat cgcagcgctc gacgctcgag gtcagcgcga
     1081 gcaggcgcca gcggccgcgc agcagcttgg ccacgcgcag gccgatgtcg ccgcagccga
     1141 cgatcagcaa ggtcggccgc cgcaagcgcg cagcgcgggc cgtcacgagg ggattggatg
     1201 tgcaggcaaa atcaccggct cagtttaatg agaacgccac gccgcgttcg ccctccatgc
     1261 ccttcaccgt caccgtgcag cccagcgggc gcagcttctc agtcgaccgc gacgagccca
     1321 ttctcagcgc cgcgatccgt cagggcatcg gcctgcccta tggctgcaag gacggcgcct
     1381 gcggctcgtg caagtgccgg ctggtcgaag gccgcgtgat cctcggggtg caccagtcca
     1441 aggccctgag cgccgacgaa gaggccgccg gctggatcct gacctgccag gccgcgccgc
     1501 agaccgacgt ggtgatcgag gcgcgcaccg tgcccggcgc cggcgagttc gcgatccgca
     1561 agatgcccac gcgcgtgacg acgatcacca agcccgcgcc cgatgtcgcg atcctgcaga
     1621 tgcagctgcc ggcgaacgac cggctgcagt accacgccgg ccagtacatc gagttcatcc
     1681 tgcgcgacgg cgcgcggcgc agctactcga tggcgaacgc gccgcacacg caggccgaca
     1741 agcccggcat cgaactgcac ctgcggcacc tgcccggcgg caagttcacc gaccacgtgt
     1801 tcacggcgat gaaggaaaag gacatcctgc gcatggaagg cccgttcggc agcttcttcc
     1861 tgcgcgagga ctccgacaag ccgatgatcc tgctcgcctc gggcaccggc ttcgcgccga
     1921 tcaaggcgat catcgagcac ctgatcgaga agggcagcca gaggaaggcc gtcctgtact
     1981 ggggctgccg ctcgaaggcc gatctctaca tgcacgactg ggccgagcag gcgacggcga
     2041 agctcgcgca cctgcgctac gtgccggtgc tctccgagc
//
LOCUS       DATMJB010000408         2061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_229486, whole genome shotgun sequence.
ACCESSION   DATMJB010000408 DATMJB010000000
VERSION     DATMJB010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229486"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VNU48_08805"
     CDS             <1..473
                     /locus_tag="VNU48_08805"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HWI11412.1"
                     /translation="AKMFLTEVFRGRYVPMPAVTEFPNAERLNELMIVGPIKVRSACS
                     HHLCPIMGKVWIGIMPNEHSNLIGLSKYARICEWIMSRPQIQEEAVTMLANELQGRVK
                     PDGLAIVMEADHFCMHWRGVKDDQSMMTNSVMRGAFLKDANLRREFLSLLSKKV"
     gene            485..913
                     /locus_tag="VNU48_08810"
     CDS             485..913
                     /locus_tag="VNU48_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HWI11413.1"
                     /translation="MLVRLMYASRAAEGLKPDAVGAILKTSTAHNPAQGVTGVLCFSG
                     KVFLQVLEGGRAQVSQLYNRIARDPRHTDVVLLSYDEIDERSFAGWAMGQVNMNKLNP
                     ALLLKYSEAAVLDPYAVSGKVSMALFNELVATASVMCLGS"
     gene            complement(867..1232)
                     /locus_tag="VNU48_08815"
     CDS             complement(867..1232)
                     /locus_tag="VNU48_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HWI11414.1"
                     /translation="MIAPPAEAGWPCLSRHGAVLWLAVSVQPNAKRTAADGLHDGALR
                     VRLGAPPVDGKANQLLVEWLADELGLPRRAVQLVRGETSRRKWLAIDAPAGQVAAWLA
                     ARLGVSCPGTSPTPSPPAR"
     gene            complement(1229..>2061)
                     /locus_tag="VNU48_08820"
     CDS             complement(1229..>2061)
                     /locus_tag="VNU48_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HWI11415.1"
                     /translation="GNWEFQLSEMEHGRLPREKFMNDIAAMAERIVKKAKEYDRDTIP
                     GDYATLNTPCPNCGGIVKENYRRFSCTGKAGAAEGCGFSISKIPGSRAFELDEVEQFL
                     THKKIGPLEGFRSKAGWPFTAELRLVFDDEVKNWKLEFDFGEDAKKDGESGEPIDFSG
                     QESLGPCPKDQGHVYEHGASYVCEHAVGAHVTCDFKSGKIILQQPVAREQMSKLLATG
                     KTELLEGFVSNKTRRKFKARLAYDKKEGKVTFEFEPRTPRVPAKGAAAAKKTAAKKAA
                     "
BASE COUNT          311 a          708 c          683 g          359 t
ORIGIN      
        1 tcgccaagat gttcctgacc gaggtgttcc gcggccgcta tgtgccgatg cccgcggtca
       61 ccgagttccc gaatgccgag cggctgaacg agctgatgat cgtcggcccg atcaaggtgc
      121 gcagcgcctg ctcgcaccac ctgtgcccga tcatgggcaa ggtctggatc ggcatcatgc
      181 cgaacgagca ctcgaacctg atcggtctct ccaagtacgc gcgcatctgc gaatggatca
      241 tgagccgccc gcagatccag gaagaggcgg tgacgatgct ggccaacgaa ctgcagggcc
      301 gcgtcaagcc ggacggcctg gccatcgtga tggaggccga ccacttctgc atgcactggc
      361 gcggcgtcaa ggacgaccag tcgatgatga cgaacagcgt gatgcgcggc gcgttcctga
      421 aggacgcgaa cctgcggcgc gaattcctgt ccctgttgag caagaaagtc tgaggagctc
      481 tgccatgctg gtccgactga tgtacgcgag ccgcgccgcc gagggcctga agccggatgc
      541 cgtgggcgcg atcctgaaga cgtccaccgc gcacaacccg gcgcagggcg tcaccggcgt
      601 gctgtgcttt tccggcaagg tcttcctgca ggtgctcgaa ggcgggcgcg cgcaggtcag
      661 ccagctctac aaccgcatcg cacgggaccc gcgccacacc gacgtggtgc tgctcagcta
      721 cgacgagatc gacgagcgca gcttcgccgg ctgggcgatg ggccaggtca acatgaacaa
      781 gctcaacccc gcgctgctgc tgaagtattc cgaggccgcc gtgctcgacc cgtacgcggt
      841 gtcgggcaag gtctcgatgg cgctgttcaa cgagctggtg gcgacggcgt cggtgatgtg
      901 cctgggcagc tgacgccgag gcgcgccgcg agccaggccg cgacctgccc ggccggcgcg
      961 tcgatcgcga gccacttgcg ccgcgaggtt tcgccgcgca ccagctgcac cgcgcggcgc
     1021 ggcaggccga gctcgtcggc gagccactcg accagcagct gattcgcctt gccgtcgacc
     1081 ggcggcgcac ccaggcgcac gcgcagggcg ccgtcgtgca ggccatcggc tgcggtgcgc
     1141 ttcgcgttcg gctgcacgct gaccgccagc cacagcaccg cgccgtggcg cgacaggcag
     1201 ggccagcccg cctcggccgg cggcgcgatc acgccgcctt cttggccgcc gtcttcttcg
     1261 ccgccgccgc gcccttggcg ggaacgcgcg gcgtgcgcgg ctcgaactcg aacgtgacct
     1321 tgccttcctt cttgtcgtag gccagccgcg ccttgaactt gcggcgcgtc ttgttcgaga
     1381 cgaagccttc gagcagctcg gtcttgccgg tggccagcag cttgctcatc tgttcgcgcg
     1441 cgaccggctg ctgcaggatg atcttgccgc tcttgaagtc gcaggtcacg tgggcaccga
     1501 ccgcgtgctc gcagacgtag ctcgcgccgt gctcgtagac gtggccctgg tccttcgggc
     1561 atgggcccag gctctcctgg cccgagaagt cgatcggttc gcccgactcg ccgtccttct
     1621 tggcgtcctc gccgaaatcg aactcgagct tccagttctt gacctcgtcg tcgaacacga
     1681 gccgcagctc ggcggtgaac ggccagcccg ccttcgagcg gaagccttcc agcgggccga
     1741 tcttcttgtg cgtcaggaat tgctcgacct cgtcgagttc gaacgcgcgg ctgccgggga
     1801 tcttgctgat gctgaaaccg cagccctcgg ccgcaccggc cttgccggtg cagctgaaac
     1861 ggcggtagtt ctccttcacg ataccgccgc agttcgggca cggcgtgttc agcgtcgcat
     1921 agtcgccggg gatggtgtcg cggtcgtact ccttggcctt cttgacgatg cgctcggcca
     1981 tcgccgcgat gtcgttcatg aacttctcgc gcggcagccg gccgtgctcc atctcggaca
     2041 gctggaactc ccagttgccg g
//
LOCUS       DATMJB010000409         2940 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_770042, whole genome shotgun sequence.
ACCESSION   DATMJB010000409 DATMJB010000000
VERSION     DATMJB010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770042"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VNU48_08825"
     CDS             <1..578
                     /locus_tag="VNU48_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HWI11416.1"
                     /translation="WIALGLNAAMFAVEIVGSLRSGSVSLLADAIDFFGDAANYGLSL
                     AVLSMALTVRARAALLKAAAMAGFGVFVLGRALWSAWNGVAPEALTMGAVALLALAVN
                     VAVAWLLYRHREGDANRRSVWLCSRNDALGNLAVALAALGVAGSGAAWPDLLVAAGMA
                     ALALASAWSVMRQALVELRASRLAPGTFAQD"
     gene            742..1719
                     /locus_tag="VNU48_08830"
     CDS             742..1719
                     /locus_tag="VNU48_08830"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HWI11417.1"
                     /translation="MEIFDYDNVLLLPRKCRVDSRSECDASQQFGRHRFNLPVVPANM
                     KTVVDEPITEWLASNGYFYVMHRFDVDNVAYARKMRDKGLIVSLSSGVKPADRAVIDR
                     LALEGVGADYITIDIAHGHADSVHRMIEHIKKKLPATFVIAGNVATPEAVIDLENWGA
                     DATKVGVGPGKVCITKLKTGFGTGGWQLSALKWCARVATKPIIADGGIRDHGDIAKSV
                     RFGASMVMIGSLFAGHEESPGKTVEVDGKLFKEYYGSASDFNKGEYKHVEGKRILEPI
                     KGKLADTLREMREDVQSSISYAGGTKLIDVRKVNYVILGGDNAGEHLLM"
     gene            1792..2094
                     /locus_tag="VNU48_08835"
     CDS             1792..2094
                     /locus_tag="VNU48_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWI11418.1"
                     /translation="MLATLTSKGQVTLPKEIRDALNLSSGAKLDFSLQPDGSVNVRPM
                     KSSVASLFGILKRPGGKAGTIEEQKDSVGRALAEKHDRILREAALPPTGKSRKRTP"
     gene            2091..2486
                     /locus_tag="VNU48_08840"
     CDS             2091..2486
                     /locus_tag="VNU48_08840"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWI11419.1"
                     /translation="MIGIDTNVLLRLLLDDDPSQVRRARHEASSAEAAGQPLFVNDVV
                     LVETVWTLGSRYDATKPELIETLRSLLDNARLTFENRPVLSDAVAGFERSSADFADCL
                     IVAKNAAAGCRHTATFDRALRPFEQAKIL"
     gene            2494..>2940
                     /locus_tag="VNU48_08845"
     CDS             2494..>2940
                     /locus_tag="VNU48_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWI11420.1"
                     /translation="MTELAKSFEPQAIEARWALKWNEPGAFAPTLDPAKPSFSIQLPP
                     PNVTGTLHMGHGFNHTIMDALTRYHRMRGFNTLWLPGTDHAGIATQIVVERQLQDEGK
                     SRHDIGRENFIARVWEWKEHSGNTITAQMRRLGDTVDWSREYFTMDA"
BASE COUNT          520 a          972 c          989 g          459 t
ORIGIN      
        1 tgtggatcgc actggggctg aacgcggcga tgttcgcggt cgagatcgtc ggcagcctgc
       61 gatcgggctc ggtctcgctg ctcgccgacg cgatcgactt cttcggcgac gcggcgaact
      121 acggcctgtc gctggccgtg ctgtcgatgg cgctcacggt gcgcgcgcgc gccgcgctgc
      181 tgaaggccgc ggcgatggcc ggcttcggcg tgttcgtgct cggccgcgcg ctgtggagcg
      241 cctggaacgg cgtggcgccg gaggcgctga cgatgggtgc ggtggcgctg ctggcgctgg
      301 ccgtcaacgt cgcggtcgcg tggctgctgt accgccaccg cgagggcgac gcgaaccggc
      361 gctcggtctg gctgtgctcg cgcaacgacg cgctcggcaa cctcgcggtc gcgctggccg
      421 cgctcggcgt ggccggcagc ggcgccgcct ggcccgacct gctggtggcc gccgggatgg
      481 ccgcgctcgc gctcgccagc gcctggtcgg tgatgcgcca ggcgctcgtc gagttgcgcg
      541 cgtcccgcct ggcacccggc accttcgctc aggactgacc ggccgcgccg agccgcgtag
      601 aattcgccac cgggcccaaa ttttggcgcc cggttttttt tggcgcgtgc tgcgccgaca
      661 ccgggttctc caagtcaagc gctcgcagcc tgagttcgca cactgcctca acgtgcggat
      721 cactcccaag gagcgagaac catggaaatc ttcgactacg acaacgtcct gctgctgccg
      781 cgcaaatgcc gcgtcgacag ccgcagcgaa tgcgacgctt ctcagcagtt cggccgccac
      841 cgcttcaacc tgccggtggt gccggccaac atgaagacag tggtcgacga gccgatcacc
      901 gaatggctcg cgtcgaacgg ctacttctac gtgatgcatc gcttcgacgt cgacaacgtc
      961 gcctacgcgc gcaagatgcg cgacaagggc ctgatcgtct cgctcagctc gggcgtgaag
     1021 ccggccgacc gtgcggtgat cgaccgcctc gcgctcgagg gcgtcggcgc cgactacatc
     1081 acgatcgaca tcgcgcacgg ccacgccgac agcgtgcaca ggatgatcga gcacatcaag
     1141 aagaagctgc cggccacgtt cgtgatcgcc ggcaacgtcg ccacgcccga ggcggtgatc
     1201 gacctggaga actggggcgc cgacgcgacc aaggtcggcg tcggcccggg caaggtctgc
     1261 atcacgaaac tgaagaccgg cttcggcacc ggcggctggc agctctcggc gctgaagtgg
     1321 tgtgcgcggg tcgcgaccaa gccgatcatc gccgacggcg gcatccgcga ccacggcgac
     1381 atcgcgaaga gcgtgcgctt cggcgcctcg atggtgatga tcggctcgct gttcgccggc
     1441 cacgaggagt cgccgggcaa gacggtcgag gtcgatggca agctgttcaa ggagtactac
     1501 ggctcggcca gcgatttcaa caagggcgag tacaagcacg tcgagggcaa gcgcatcctc
     1561 gagccgatca agggcaagct cgccgacacg ctgcgcgaga tgcgcgagga cgtgcagagc
     1621 tcgatctcgt atgcgggcgg cacgaagctg atcgacgtgc gcaaggtcaa ctacgtgatc
     1681 ctgggcgggg acaacgcggg cgagcacctg ctgatgtgag cctgcagcgc gggcagtgtg
     1741 aatctcctta ccagatagaa ttggcaagga attcacttcg ccggagccac catgcttgcc
     1801 acccttacca gcaaaggtca agtcacgttg ccgaaggaaa ttcgtgatgc actgaacctc
     1861 agttcgggcg ccaagctgga tttctcgctg caacccgatg gcagcgtcaa cgttcgaccg
     1921 atgaagagtt cggtggcttc cctgttcggc atcttgaagc ggccgggcgg caaggccggc
     1981 acgatcgagg agcagaagga ctcggtcggg cgcgccttgg ccgagaagca tgaccgcatc
     2041 ctccgagaag cggcgcttcc tccgaccggg aagtcgcgga agcgcacgcc gtgatcggca
     2101 tcgacaccaa cgtgctgttg cggctgctgc tggacgacga cccctctcaa gttcgacggg
     2161 ctaggcatga agcgtcgagc gccgaagcgg ctggccagcc gttgttcgtc aacgacgtgg
     2221 tgctggtcga aacggtctgg acgctcgggt cacgatacga cgcgaccaag cccgagctga
     2281 tcgaaaccct gcgcagcttg ctcgacaatg cgagactgac cttcgaaaac cggccggtgt
     2341 tgagtgacgc ggtggccggg ttcgagcgct ccagcgccga ctttgccgac tgtctcatcg
     2401 tcgcgaagaa cgcggcggcc ggctgtaggc acaccgccac cttcgatcgg gcgctgcgcc
     2461 cgttcgagca agccaagatc ctctgacgcc accatgaccg aactcgccaa atccttcgaa
     2521 ccgcaagcca tcgaggcgcg ctgggcgctc aagtggaacg agcccggcgc cttcgccccc
     2581 acgctcgatc cggccaagcc gtcgttcagc attcagctgc cgccgccgaa cgtgaccggc
     2641 acgctgcaca tggggcacgg cttcaaccac acgatcatgg atgcgctgac gcgctaccac
     2701 cggatgcgcg gcttcaacac gctgtggctg ccgggcaccg accacgccgg catcgcgacg
     2761 cagatcgtcg tcgaacgcca gctgcaggac gaaggcaaga gccggcacga catcggccgc
     2821 gagaacttca tcgcgcgcgt gtgggaatgg aaggagcaca gcggcaacac catcaccgcg
     2881 cagatgcgcc ggctgggcga cacggtcgac tggtcgcgcg agtacttcac gatggacgcg
//
LOCUS       DATMJB010000410        12730 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_491797, whole genome shotgun sequence.
ACCESSION   DATMJB010000410 DATMJB010000000
VERSION     DATMJB010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 12730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12730
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491797"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..6822
                     /locus_tag="VNU48_08850"
     CDS             31..6822
                     /locus_tag="VNU48_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HWI11421.1"
                     /translation="MVTLDGDTQLPPGRLRELVGVAAHPHNQPQVDPVTRTVTQGYGI
                     LQPRVLTPLPTPKNFTLYHWLFAGQCGIDPYSAASSEVYQDVFREGTFTGKGLLHVGA
                     VHAVLSGRLPDGQVLSHDLLEGSLARCAAVTDITVIEDAPFHADVAASRVHRWTRGDW
                     QLLPFLLNPARYGIRAINRWKMIDNLRRSLVAPMSLALLALALYSSVVAPPAALALVL
                     AALIAGPLMGAVAGLAPSHDGIAKLHFYRLALADLKRAVCSGLWLLAQLLQLALLNLD
                     ALLRALWRMAVSRRHLLEWTTAAAAQASASIEWREIVRRHRAVPLAAAALWIALLAGH
                     TPAPGLATLLCAVWAATPLWTWWVSRPRPPKRDAALPAADRRYLEDAARDTWRLFERC
                     VGPEDNFLPPDNLQLTPQTLVAHRTSPTNIGLYLLSAACARRFGWIGTQDLLGRLEAT
                     LATLAELPRHRGHFLNWYDTQHKAALLPMYVSTVDSGNLSGHLLAVAQACIELARAPY
                     DTGAARRALAASNARLEPLRAAATEALPDGALTRLLALADPLADALARPDWLEGLLRS
                     ASDELAGLLPDDRAALAPGTLDEFAWRAADHVVTLRSALFDAQAMRAEKPDADATERI
                     GFDGGAMQRLQSVAARCDALAWEADYAFLYHRKRHLFHIGYRVAEQQLDASFYDLLAS
                     ESRLTSLLAIAKGDVPVKHWAALGRPFYAVGMHAGLRSWSGSMFEYLMPTLLLDEPHG
                     SVLREAGHAAICEQVAFGREQHVPWGISESAYAASDHTLAYQYAPQGVPRLALRRTPP
                     DELVIAPYATALAAQITPHLAVANLARLDALTPRARYGFIEALDFSPGRQQSATEGFT
                     RVDTFMAHHQGMSIVALANVLLDGTPRRWAMANAHIEAMASLLHERAPREVSVLLEPP
                     PGPSPEARQRRVPGMLRELVPGLTALAPTHLLSNGRYSVSLRPNGAGRSRWEQVGVSR
                     WRDDALRDAHGSFFYLRWDRQPQAVSLTQHPAPDPAAHYQSTFHADRVCFDARWREVA
                     TRATVWVGPEDDIEFRQVELRNLSERSLDLELISAFEITLAPAAADEAHPAFSNLFLR
                     SAWQPAQQALRFERKPRLPTEAGLYAAHFLAESDPAVTALRVQTDRLGWLGRNRHASQ
                     PLASFPPLPQADAGGAAELPLDTGLDPVCALSVRLQLAPNSRLVLTFCTAASDQRATL
                     DAVIDKHRQRSPVERASLMSATLTGIRLRELAINAENHAAIQALTTAVVMTLTRPRAY
                     DALPDAAGDDARAPPCDRRLLWRFGISGDRPIVLVSAGAPQGLGLLRTLVQALRVWSW
                     SGVSCDLVVVNAEPSSYLMALQRDVAALRDQFTAQNSGAPGAGVTSFHVLRADELSAD
                     ELGTLRTLARVRFNADGRPLTHHVQEWCEQHEAAFEERHAGSITALPIAGGGAGAPPR
                     VSQGRFMPEGGEFRFDVSVFQRPARPWVNVLANPDFGTQLSEAGGGYTWALNSRLNQL
                     TPWSNDALADPPGEWFLLQDQRTHDLWSISPSAWGDPSATYRVAHGQGYSAIGHRRGE
                     LEVSAVWSVDPESALKQLRLRLVNRGHRTQRLRVIGIAEWIMGASRGDRASTDTASFT
                     QRLSGAARGGADGDATPRLTALTCTQNDRSAGFGAGTAFLALRTDADDTPDWTCDRRE
                     CFDARGRLALPDAFGQHSGAGLDPCAALSTSVSIAAGETAECVFLLGFGATPADARRL
                     ATTAAAHSATERLDATRMHWDALLGATTVKTPDPLFDALVNRWLLYQTIACRLWAKAG
                     FYQAGGAFGFRDQLQDAMALAWAAPDMLRRQIVLNASRQFAEGDVQHWWHAPTGAGVR
                     THFSDDLLWLPHACAHYLARTGDATLLDEQVAFIEGGPIPDGAEDAYYVPTPGTGTAS
                     VFEHGARAIDRSLKVGAHGLPLMGSGDWNDGMNRVGAEGRGESVWLAWFLCQIVDAYA
                     PIARGRGETARAQRWEAAGRQWRNALQREAWDGQWFKRAFFDDGTPLGSNVNRECRID
                     LIAQAWSAISDAAPAAFRRMALSAMDKHLVDTDAGLIKLLTPALQTQQPSAGYIQAYP
                     PGVRENGGQYSHAGVWALIAQALGGDGDTAYRYFTYLSPAHRAADPVRGPAYAIEPYV
                     MAGDVYAEPPYVGRGGWSWYTGSAAWMHRAAIEHLFGLRQHGDEIGFTPCLPSHWNVA
                     ELTLRRGNRRLRVIFRRGASASAATAAAEMLELRRGEALHWDRLANDSTWLMRLEALE
                     PSIAAPAASTAPTLP"
     gene            6955..7524
                     /locus_tag="VNU48_08855"
     CDS             6955..7524
                     /locus_tag="VNU48_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWI11422.1"
                     /translation="MSAKKPSGNVTALPVATERLVLDEAALIAAKRSLDQGAVVPSYG
                     PWREDIIKLLNDALATEIVCVMRYKRHHFTAKGLASPKIAEEFLVHAAEETVHGDQIA
                     ERIVQLGGEPDFSPSTLLARSHADYDASLDLKAMIKANLIAERVAIEAYTQMIALIGD
                     KDSTTRRMLESILSQEQEHAEELSDWMTE"
     gene            7600..7914
                     /locus_tag="VNU48_08860"
     CDS             7600..7914
                     /locus_tag="VNU48_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWI11423.1"
                     /translation="MQLRTTLASAIAVIALVTVSGCAVQRGQETVGAYVDDAAITTSV
                     KARFVDNSAVDAGAIKVETLNGTVMLSGFAKNSTERATAEDIASKVKGVKHVKNEIAV
                     RP"
     gene            8041..9192
                     /locus_tag="VNU48_08865"
     CDS             8041..9192
                     /locus_tag="VNU48_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWI11424.1"
                     /translation="MSAVGHENAAPTAPVHSVATTVLATLAIVLALWWGRSFLVPVTA
                     GLMLALLVTPLTTLLTRWLRLRFLAVLVTLAIVVGVIGAAGAAFGDQLGRVAGRVPDM
                     ISLVAQQVAETDPGADSVLRRARDAFRELDRAVWISGRAAAAKQAAAAASAAAAAEPG
                     VNITETATVVLRESAVSGSSALLKLAGDLTVILFVAFFVLVGGAPLATRFLDLWDARP
                     GLRLRAEHAATECVRQIHIYGGVLLVTNSIVGLGVWAAFTFAGLPDAGGWGLTAAVLH
                     VVPYLGMALLTGLGAAEAYLARGTVGAALGMAAFVVVLSSLIGTVVTAWLQGRAAKMN
                     SAAVFLGVVFWGALWGVWGLLLGPALVVLMKVIAEHTRPGQRLAHLLKG"
     gene            complement(9208..9816)
                     /locus_tag="VNU48_08870"
     CDS             complement(9208..9816)
                     /locus_tag="VNU48_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11425.1"
                     /translation="MPSPTPLFLLLIGLAVTPAAHADADDEPAGPTSVAALASSVAGG
                     GSNDAPRLVRSDDPAEPRRSAPGLRGETTELGYRWWLSKGRADLGLGLGTLGYAVRPT
                     GSLPGLGDNGATVLASGTVLTLGMRYRTSASSAVFADAASWHGSGLNGDAVVGKVGLE
                     FKTAKSTLNLAYGGLGLALPGDARMLLRVRRGGFGLALRSSF"
     gene            complement(9848..10048)
                     /locus_tag="VNU48_08875"
     CDS             complement(9848..10048)
                     /locus_tag="VNU48_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11426.1"
                     /translation="MYRERQHDAGFKSDRQPTRKEWGHGGEEQHQLEARERARQPGRM
                     LRTHEVRAAEAERQIRELRDRY"
     gene            10346..10588
                     /locus_tag="VNU48_08880"
     CDS             10346..10588
                     /locus_tag="VNU48_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11427.1"
                     /translation="MNKPTPDKDLQGEGNYDATRRYDKAAREFVQSGKVDDAAQAAEP
                     GSPEQAESLKRAEEVGKSHSKGEDPAVRKASPSPKK"
     gene            complement(10680..11231)
                     /locus_tag="VNU48_08885"
     CDS             complement(10680..11231)
                     /locus_tag="VNU48_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11428.1"
                     /translation="MITLQHCKTGIAIAAMFAAAGAFAATGDKTDYQAAKDRIGAEYK
                     ADKAACDKFSGNQKDICVEQAKGKEKVALAEAEYNYTGKPADATKVAEAKADASYAVA
                     KEMCDDKGGNAKDVCMTEAKSAHTKALADAKMDKKVGEARKDAADDKRDADYRLATEK
                     CDSLAGDAKSACISAAKAKFGKS"
     gene            complement(11340..>12730)
                     /locus_tag="VNU48_08890"
     CDS             complement(11340..>12730)
                     /locus_tag="VNU48_08890"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWI11429.1"
                     /translation="PVIADDAVTQALPVTLDAHIGGASVTFRGTATDALHLSALQGRF
                     SVQGPSLAAIGDPLHVTLPTTGPFRAQGVIAKQGLVWNTVVEAATIGSSRLAGAFTYD
                     PRSARPVLAGRLTGTKLLLVDLGPAFGTTAKKSAAVPEVTPAAPRARAAPGHVLPNRE
                     FDLPSLRAMDANVLIDIDTLDLGSGFLEPLKPLRTHLVLIDGVLTLRELEARTGQGRL
                     FGTVQLDGRNAQALWTADLRWSGVRLESWIHQARADNAPPYTTGSLSGQARVAGQGKS
                     TAAILGSLRGGVRMQLVDGTISHLAVEAAGLDIAQGLGVLIKGDDALPVQCSVVDLVA
                     DQGLLKPRVLVLDTPDSTLWVDGSLSLASETMDLRVVTPPKDFSPLARRTPVRLRGSF
                     ASPAISVEKGPLGARVGASLLLGLLNPLAALVPLMDVGSSDDAKRGADACRALSQRIA
                     ARPLLPAPPRAR"
BASE COUNT         1748 a         4716 c         4467 g         1799 t
ORIGIN      
        1 tggtcgcgcg tcgaacccgc gacgcgctac gtggtcacgc tcgacggcga cacgcagctg
       61 ccgccggggc ggctgcgcga gctggtgggc gtggcagcgc acccgcacaa ccagccgcag
      121 gtcgacccgg tgacgcgcac cgtgacgcag ggctacggca tcctgcagcc gcgcgtgctg
      181 acgccgctgc cgacgccgaa gaacttcacg ctgtaccact ggctgttcgc aggccagtgc
      241 ggcatcgacc cgtacagcgc ggccagctcc gaggtctacc aggacgtgtt ccgcgaaggc
      301 accttcaccg gcaaggggct gctgcacgtc ggcgccgtgc acgcggtgct gtcgggtcgc
      361 ctgcccgacg gccaggtgct gagccacgac ctgctcgaag gctcgctggc gcgctgcgcc
      421 gccgtcaccg acatcacggt gatcgaggac gcgccattcc acgccgacgt cgccgcctcg
      481 cgcgtgcacc gctggacgcg cggcgactgg cagctgctgc cgttcctgct gaacccggcc
      541 cgctatggca tccgtgcgat caaccgctgg aagatgatcg acaacctgcg gcgctcgctg
      601 gtcgcgccga tgtcgctggc gctgctcgca ctcgccctct acagcagcgt cgtcgcaccg
      661 ccggccgcgc tcgcgctggt gctggcggcg ctgatcgccg ggccgctgat gggcgcggtg
      721 gcgggcctcg cgccgagcca cgacggcatc gccaagctgc acttctaccg cctggccctt
      781 gccgacttga agcgcgccgt ctgcagcggc ctgtggctgc tggcccagct gctgcagctc
      841 gcgctgctga acctggacgc gctgctgcgc gcactctggc gcatggcggt gagccggcgc
      901 cacctgctcg aatggaccac cgccgcggcg gcgcaggcct cggcctcgat cgagtggcgc
      961 gaaatcgtgc gccgccatcg cgccgtgccg ctggccgccg cggcgctgtg gatcgcgctg
     1021 ctcgccgggc acacgccggc gcccggcctg gcgacgctgc tgtgtgcggt ctgggcggcc
     1081 acgccgctgt ggacctggtg ggtcagccgc ccgcgcccgc caaagcgcga cgccgcgctg
     1141 ccggccgccg accggcgcta cctcgaagac gccgcgcgcg acacctggcg cttgttcgag
     1201 cgctgcgtcg gccccgaaga caacttcctg ccgcccgaca atttgcagct gacgccgcag
     1261 accctggtcg cgcaccgcac ctcgccgacc aacatcggcc tgtacctgct cagcgcggcc
     1321 tgcgcgcggc gcttcggctg gatcggcacg caagacctgc tggggcggct cgaagccacg
     1381 ctggcgacgc tggccgagtt gccgcgccac cgcggccact tcctgaactg gtacgacacg
     1441 cagcacaagg cggcgctgct gccgatgtac gtgtccacgg tcgacagcgg caacctcagc
     1501 ggccacctgc tggcggtggc gcaggcctgc atcgaactcg cacgcgcgcc ctacgacacg
     1561 ggcgcggcgc gccgcgcgct ggcggcttcg aacgcgcgcc tcgagcccct gcgcgcggcc
     1621 gcgaccgagg cgctgcccga cggtgcactg acgcgcctgc tcgcgctcgc cgacccgctg
     1681 gccgacgcgc tggcccgccc ggactggctc gaaggcctgc tgcgcagcgc ctccgacgaa
     1741 ctcgccggcc tgctgccgga cgaccgcgcg gcgctcgcgc ccggcacgct cgacgagttc
     1801 gcctggcgcg ccgccgacca cgtcgtcacg ctgcgcagtg cgctgttcga tgcgcaggcg
     1861 atgcgcgccg agaagcccga cgccgatgcc actgagcgga tcggcttcga cggcggcgcg
     1921 atgcagcgcc tccagtcggt cgcggcccgt tgcgacgcgc tcgcctggga ggccgactac
     1981 gccttcctgt accaccgcaa gcggcacctc ttccacatcg gctaccgcgt cgccgagcag
     2041 cagctcgacg cgagcttcta cgacctgctc gcgtctgagt cgcgcctgac cagcctgctg
     2101 gcgatcgcca agggcgacgt gccggtgaag cactgggccg cgctcggccg gccgttttac
     2161 gcggtcggca tgcacgccgg gctgcgctcg tggtcgggct cgatgttcga gtacctgatg
     2221 cccacgctgc tgctcgacga gccgcacggc agcgtgctgc gcgaggccgg ccacgcggcg
     2281 atttgtgagc aggtggcgtt cgggcgcgag caacacgtgc cctggggcat ctccgagtcg
     2341 gcctacgcgg ccagcgacca cacgctggcc taccagtacg caccgcaggg cgtgccgcgg
     2401 ctggcattgc gccgcacccc gcccgacgag ctggtgatcg caccctacgc caccgcgctg
     2461 gcggcgcaga tcacaccgca cctggcggtc gcgaacctgg cgcggctgga tgcgctgacg
     2521 ccgcgcgcgc gctacggctt catcgaggcg ctcgacttca gccccggccg ccagcagtcc
     2581 gcgaccgaag gcttcacgcg cgtcgacacc ttcatggcgc accaccaggg catgagcatc
     2641 gtcgcgctcg ccaacgtgct gctcgacggc acgccgcggc gctgggcgat ggccaatgcg
     2701 cacatcgagg cgatggcctc gctgctgcac gaacgcgcgc cgcgcgaggt ctcggtgctg
     2761 ctcgagccgc cgccggggcc gtcgcccgag gcacgccagc gccgcgtgcc gggcatgctg
     2821 cgcgagctcg tgccgggcct gacggcgctg gcgccgacgc acctgctctc gaacggccgc
     2881 tacagcgtct cgctgcgccc caacggcgcc gggcgcagcc gctgggaaca ggtcggcgtg
     2941 tcacgctggc gcgacgacgc gctgcgcgac gcgcacggca gcttcttcta cctgcgctgg
     3001 gatcgccagc cgcaggcggt gtcgctgacg cagcacccgg cgcccgaccc ggccgcgcac
     3061 taccagagca ccttccacgc cgaccgcgtc tgcttcgacg cgcgctggcg cgaggtcgcg
     3121 acccgcgcga cggtctgggt cggccccgag gacgacatcg aattccgcca ggtcgagctg
     3181 cgcaacctga gcgaacgcag cctcgacctg gagctgatct cggcgttcga gatcacgctg
     3241 gccccggcgg cggccgacga ggcgcatccg gcgttctcga acctgttcct gcgttcggcg
     3301 tggcagccgg cccagcaggc gctgcgcttc gaacgcaagc cgcgcctgcc gaccgaggcc
     3361 ggtctgtacg cggcccactt cctggccgag agcgacccgg ccgtcaccgc gctgcgcgtg
     3421 cagaccgacc gcctgggctg gctcggccgc aaccgccacg cgagccagcc gctggcctcg
     3481 ttcccgccgc tgccgcaggc cgatgcgggc ggcgccgccg agctgccgct cgacaccggg
     3541 ctggacccgg tgtgcgcact gtcggtgcgg ctgcagctcg cgcccaacag caggctcgtg
     3601 ctcaccttct gcaccgccgc ttccgaccag cgcgccacgc tcgacgcggt gatcgacaag
     3661 catcgccagc gcagcccggt cgagcgggcc tcgctgatgt cggcgacgct gaccggcatc
     3721 cgcctgcgcg agctcgcgat caacgccgag aaccacgcgg cgatccaggc gttgacgacc
     3781 gccgtcgtga tgacactgac gcggccgcgc gcatacgatg cgctgcccga cgcagcgggc
     3841 gacgacgcgc gcgcgccgcc ctgtgaccgc cgcctgctgt ggcgcttcgg catctcgggc
     3901 gatcgcccca tcgtgctcgt ctcggccggc gcgccgcagg gcctggggct gctgcgcacg
     3961 ctggtgcagg cgctgcgcgt ctggtcgtgg tcgggcgtga gctgcgatct ggtcgtcgtc
     4021 aacgccgagc ccagttcgta cctgatggcg ctgcagcgcg atgtcgccgc gctgcgcgac
     4081 cagttcaccg cgcagaacag cggcgcgccc ggcgccggcg tcaccagctt ccacgtgctg
     4141 cgtgccgacg aactcagtgc cgacgaactc ggcacgctgc gcaccctggc gcgcgtgcgc
     4201 ttcaacgccg acggccggcc gctcacgcac cacgtgcagg aatggtgcga gcagcacgag
     4261 gccgcgttcg aggagcgcca tgccggctcg atcacggcgc tgccgatcgc cggtggcggt
     4321 gccggcgcgc cgccgcgcgt gtcgcagggc cgcttcatgc ccgagggcgg cgaattccgc
     4381 ttcgatgtga gtgtgttcca gcgcccggcg cggccctggg tcaacgtgct cgcgaacccg
     4441 gatttcggca cccagctctc ggaggccggc ggcggctaca cctgggcgct gaacagccgg
     4501 ctgaaccagc tcacgccatg gtcgaacgac gcactcgccg acccgcccgg cgagtggttc
     4561 ctgctgcagg accagcgcac gcacgacctg tggagcatca gcccgtcggc ctggggcgac
     4621 ccgtcggcga cctaccgggt cgcgcacggc cagggctaca gcgccatcgg ccaccgccgc
     4681 ggcgagctcg aggtcagcgc ggtctggtcg gtcgacccgg agagcgcgct caagcagctg
     4741 cggctgcgcc tggtcaaccg cggccaccgc acacagcggc tgcgcgtgat cggcatcgcc
     4801 gaatggatca tgggggcgag ccgcggcgac cgcgcgagca ccgacaccgc gtcgttcacg
     4861 cagcgcctgt ccggcgcggc gcgcggcggc gccgacggcg atgccacccc gcgcctgacc
     4921 gcactgacct gcacccagaa cgaccgctcg gccggcttcg gcgcgggcac cgcgttcctc
     4981 gcgctgcgca ccgatgccga cgacacgccc gactggacct gcgaccgccg cgagtgcttc
     5041 gacgcgcgcg gccgcctcgc gctgcccgac gccttcggcc agcacagcgg cgccggcctg
     5101 gacccctgcg ccgcgctgtc gacctcggtc tcgatcgccg ccggcgaaac cgccgagtgc
     5161 gtatttctgc tcggcttcgg cgcgaccccg gccgacgcgc gccgcctggc aaccaccgcc
     5221 gcggcgcact cggcgaccga gcgcctggac gcgacccgca tgcactggga cgcgctgctc
     5281 ggcgccacca cggtgaagac gcccgacccg ctgttcgatg cgttggtgaa ccgctggctg
     5341 ctctaccaga cgatcgcgtg ccgactgtgg gccaaggccg gcttctacca ggccggtggc
     5401 gcgttcggct tccgcgacca gctgcaggac gcgatggcgc tggcctgggc cgcgcccgac
     5461 atgctgcggc ggcagatcgt gctgaacgcg tcgcgccagt tcgccgaggg cgacgtgcag
     5521 cactggtggc atgcacccac cggcgccggc gtgcgcactc atttctccga cgacctgctg
     5581 tggctgccgc acgcctgcgc gcactacctc gcgcgcaccg gcgacgccac gctgctcgac
     5641 gagcaggtcg cattcatcga gggcggcccg attcccgacg gcgccgaaga tgcgtactac
     5701 gtgcccacgc cgggcaccgg cactgccagc gtgttcgaac acggcgcccg cgcgatcgac
     5761 cgcagcctga aggtcggcgc acacggcctg ccgctgatgg gcagcggcga ctggaacgac
     5821 ggcatgaacc gggtcggcgc cgagggccgc ggcgaatcgg tctggctggc gtggttcctg
     5881 tgccagatcg tcgacgccta cgcgccgatc gcgcgcggcc gcggcgagac cgcgcgggcc
     5941 cagcgctggg aagcggccgg ccgccaatgg cgcaacgcgc tgcagcgcga ggcctgggac
     6001 ggccaatggt tcaagcgcgc gttcttcgac gacggcacgc cgctgggctc gaacgtcaac
     6061 cgcgagtgcc gcatcgacct gatcgcgcag gcctggtcgg cgatctcgga cgccgcgccg
     6121 gccgccttcc ggcgcatggc gctgagcgcg atggacaagc acctcgtcga caccgacgcc
     6181 ggcctcatca agctgctgac gccggcactg cagacgcagc aacccagcgc cggctacatc
     6241 caggcctatc cgcccggcgt gcgcgagaac ggcgggcagt actcgcacgc cggcgtgtgg
     6301 gcgctgatcg cgcaggcgct cggcggcgac ggcgataccg cgtaccgcta cttcacctac
     6361 ctgagcccgg cgcaccgcgc ggccgacccc gtgcgcggcc cggcgtacgc gatcgagccg
     6421 tacgtgatgg ccggcgacgt ctatgccgag ccgccctatg tcgggcgtgg cggctggagc
     6481 tggtacacgg gctcggcggc ctggatgcac cgcgccgcga tcgagcacct gttcggcctg
     6541 cgccagcacg gcgacgagat cggcttcacg ccatgcctgc cgtcgcactg gaacgtggcc
     6601 gagctgacct tgaggcgcgg caatcgccgc ctgcgcgtga tcttcaggcg cggggcatcg
     6661 gcctcggccg cgaccgcggc cgccgagatg ctcgagctgc gccgcggcga ggcgctgcac
     6721 tgggatcgtc tcgcgaacga cagcacctgg ctgatgcgcc tcgaagcgct cgagcccagc
     6781 atcgcggcgc cggccgccag caccgcgccc acgctgccct gagggccacg acggcgcgcg
     6841 gccctgtcct acagcccccc gcgcgccgct ccgatgggcc gcgcactcac cggttcttac
     6901 agttcaggca ttggccgcgc agacctcgcg cgcccgccgt ttcaaggagt cttcatgagt
     6961 gcaaaaaaac catccggcaa cgtcaccgca ctgcccgtcg ccaccgagcg cctcgtgctg
     7021 gacgaggccg cgctgatcgc ggcgaagcgc tcgctcgacc agggcgcggt ggtgccgagc
     7081 tacggcccgt ggcgcgagga catcatcaag ctgctgaacg acgcgctggc caccgagatc
     7141 gtctgcgtga tgcgctacaa gcgccatcac ttcacggcca agggcctggc gtcgccgaag
     7201 atcgccgagg agttcctggt ccacgccgcg gaggaaacgg tgcacggcga ccagatcgcc
     7261 gagcgcatcg tgcaactcgg cggcgaaccc gatttctcgc cgtcgacctt gctcgcgcgc
     7321 agccatgccg actacgacgc gtcgctcgac ctgaaggcca tgatcaaggc caacctgatc
     7381 gccgagcgcg tcgcgatcga ggcctacacc cagatgatcg cgctgatcgg cgacaaggac
     7441 tcgaccacgc gccgcatgct cgaaagcatc ctgagccagg agcaggaaca cgccgaagag
     7501 ctcagcgact ggatgaccga atgattccgg ccccggcggt ggcgggccca aggccaccgg
     7561 tcaagccata actcgtccta accactcagg agaaacacca tgcaattgcg caccacactc
     7621 gcttcggcca tcgccgtcat cgccctcgtc acggtctcgg gctgcgcagt gcagcgcggc
     7681 caggaaaccg tcggcgccta tgtcgacgac gccgcgatca cgacctcggt gaaggcgcgc
     7741 ttcgtcgaca actcggccgt cgacgccggc gcgatcaagg tcgaaacgct gaatggcacg
     7801 gtgatgctgt cgggcttcgc gaagaactcg accgagcgcg cgaccgccga agacatcgcg
     7861 agtaaggtca agggcgtcaa gcacgtgaag aacgagatcg ccgttcgtcc gtaaacgcgg
     7921 cgatgttcat cggcgcacgg ccggcgatcc gccgggcgcg cgccccacgc cccggattcc
     7981 ttgcggatcg cggggcgttc atttgtgcgt cgaaacgtcg aagcggagcg cccccatccc
     8041 atgagtgccg tcggccacga gaacgccgcc ccgaccgcgc cggtccacag cgtcgccacc
     8101 acagtgctgg cgacgctggc gatcgtcctc gcgctgtggt gggggcgcag cttcctggtg
     8161 ccggtcaccg ccgggctgat gctggcgctg ctggtgacgc cgctgaccac gctgctgacg
     8221 cgctggctgc ggctgcgctt cctggcggtg ctcgtgacgc tggcgatcgt ggtcggcgtg
     8281 atcggcgcgg ccggcgccgc gttcggggat cagctcggcc gggtcgccgg gcgcgtgccc
     8341 gacatgatca gcctggtggc gcagcaggtc gccgagaccg atcccggcgc cgattcggtg
     8401 ctgcgccgcg cgcgcgacgc gttccgcgag ctcgaccgcg cggtctggat cagcggtcgg
     8461 gccgcggcgg cgaaacaggc cgcggccgcc gcgagcgccg cggcggcggc cgagccgggc
     8521 gtcaacatca ccgagaccgc cacggtggtg ctgcgcgaga gcgcggtcag cggttcgagc
     8581 gcgttgctga agctggccgg cgacctgacc gtgatcctgt tcgttgcgtt cttcgtgctg
     8641 gtcggcggcg cgccgctggc gacgcgcttc ctcgacctct gggacgcacg gcccgggctg
     8701 cgcctgcgcg ccgagcacgc ggccaccgaa tgcgtgcgcc agatccacat ctatggcggc
     8761 gtgctgctgg tcaccaacag catcgtcggg ctgggggtct gggccgcctt caccttcgcc
     8821 ggcctgcccg acgccggcgg ctggggcctg accgcggcgg tgctgcacgt cgtgccctac
     8881 ctcggcatgg cgctgctgac cgggctgggc gcggccgagg cctacctcgc gcgcggcacg
     8941 gtcggcgcgg cgctgggcat ggcggcgttc gtcgtggtcc tgtcgagcct gatcggcacc
     9001 gtcgtgacgg cctggctgca gggccgcgcg gcgaagatga actcggccgc ggtgttcctc
     9061 ggcgtggtgt tctggggcgc gctgtggggt gtgtggggct tgctgctggg ccccgcgctc
     9121 gtggtgctga tgaaggtgat cgccgagcac acgcggcccg gccagcgcct cgcgcacctg
     9181 ttgaaaggct gagccgctgc ggccggctca gaacgaactg cgcagcgcca ggccgaagcc
     9241 gccgcggcgc acgcgcagca gcatgcgcgc gtcgccgggc agcgccaggc cgaggccgcc
     9301 gtaggccagg ttcagggtcg acttcgcggt cttgaactcg aggccgacct tgccgaccac
     9361 cgcgtcgccg ttcaggccgc tgccgtgcca gctcgcggca tcggcgaaca ccgccgaact
     9421 ggccgaggtg cggtagcgca ttcccaaggt cagcacagtg ccggacgcga gcacggtcgc
     9481 accgttgtcg cccagacccg gcaggctgcc ggtcggccgc acggcatacc ccagcgtgcc
     9541 caggcccagg ccgagatcgg cccgcccctt cgacagccac cagcggtagc cgagctcggt
     9601 ggtttcgccg cgcaagcccg gcgcgctgcg ccgcggctcg gccgggtcgt cgctgcgcac
     9661 gaggcgcggc gcgtcgttgc tgccgccacc tgccacgctg ctcgccagcg ccgcgaccga
     9721 ggtcggccct gcgggctcgt cgtcggcgtc ggcgtgcgcc gccggcgtca cggccagacc
     9781 gatcaacagc aggaacagcg gggtgggaga aggcatcgtc gcgctccgat ccgagccccg
     9841 ctgagattca ataccggtcg cgaagttcgc ggatctggcg ctcggcctcg gccgcgcgca
     9901 cctcgtgcgt gcgcagcatc cggccgggct ggcgcgcgcg ctcgcgggcc tcgagctgat
     9961 gctgctcttc gccgccatgg ccccattcct tgcgggtggg ctggcggtcc gacttgaagc
    10021 cggcgtcgtg ttggcgttcg cggtacatgc gatgctccat caagcgggtg tgtggcggcg
    10081 tggcggacaa cccccgcgcc tgttcgtggg agaagcgtat cgagctgctg ccgcggccgc
    10141 ggtcggaccg cgccgaaagc atcgtaggac ggtgccgaca gttcggcatc ccctcggatc
    10201 cggggcggca cgggcttcac cgcaaggtcc ctcttcatcg gcgtctctgt cggacggcgc
    10261 ctaccggcgc aggcggcccg cgccgctcgc gcgaaggcgg cgcgcttcct atggttgctg
    10321 ttcccccaac agccgaggaa cgacagtgaa caagcccact cccgacaagg atcttcaagg
    10381 cgaaggcaac tacgacgcga cgcgacgcta cgacaaggcc gcacgcgagt tcgtgcagtc
    10441 cggcaaggtc gacgacgctg cacaggcggc cgagcccggc agcccggaac aggccgagag
    10501 cctgaaacgt gcggaagaag tcggcaagtc acattcgaag ggtgaagatc cggcggtcag
    10561 gaaggcgtcg ccctcaccga agaagtgaag cctgccgcgg atcactgcga tccgcctccg
    10621 acaagcgaaa gccggcgcga acgccggctt tctttgctcg acccgcccga cggcgtcgct
    10681 cagctcttgc cgaacttggc cttggccgcg ctgatgcagg ccgacttcgc gtcgccggcg
    10741 aggctgtcgc acttctcggt ggcgagtcgg tagtcggcgt cgcgcttgtc gtcggccgcg
    10801 tccttgcgcg cctcgccgac ctttttgtcc atcttcgcgt cggccagcgc cttggtgtgg
    10861 gccgacttgg cctcggtcat gcagacatct ttcgcgttgc cgcccttgtc gtcgcacatt
    10921 tccttggcga ccgcgtagct cgcgtcggcc ttcgcttccg cgaccttggt ggcatcggcg
    10981 ggcttgccgg tgtagttgta ttcggcctcg gcgagcgcga ccttttcctt gcccttggcc
    11041 tgctcgacgc agatgtcctt ctggttgccg ctgaacttgt cgcaggccgc cttgtcggcc
    11101 ttgtactcgg caccgatgcg gtccttcgcg gcctggtagt cggtcttgtc gccggtcgcg
    11161 gcaaacgcgc cagcggcggc aaacatcgcg gcgatcgcga tcccggtctt gcagtgttga
    11221 agcgtgatca tcgtgctgtc cctttcatgt cggcggtcga cgaccgcggt gacagaagta
    11281 tgaaaaccgc gccgcacggg ggccagcgga cggtgccgcg gcgccgagta gttcccgtcc
    11341 taccttgcgc gcggcggcgc cggcagcagc gggcgcgcgg cgatgcgctg cgacagcgca
    11401 cggcaggcgt cggcaccgcg cttggcgtcg tcgctgctgc cgacgtccat cagcgggacc
    11461 agcgccgcca gcgggttcag cagccccagc agcagcgacg cgcccacgcg cgcgcccagc
    11521 ggccccttct cgaccgagat cgccgggctg gcaaagctgc cgcgcaggcg caccggggtg
    11581 cgccgcgcga gcgggctgaa gtccttcggc ggggtgacga cgcgcaggtc catggtctcg
    11641 ctggccagcg acagcgagcc gtcgacccag agcgtcgagt cgggcgtgtc gagcacgagc
    11701 acgcgcggct tcagcaggcc ctggtccgcg accaggtcga ccaccgagca ctgcaccggc
    11761 agcgcgtcgt cgcccttgat cagcacgccc aggccctgcg cgatgtccag ccccgcggct
    11821 tcgacggcga ggtgcgagat cgtgccgtcg accagttgca tgcgcacgcc gccgcgcaga
    11881 ctgcccagga tcgccgcggt cgacttgccc tggccggcga cgcgcgcctg gccgctgaga
    11941 ctgccggtcg tatacggcgg cgcgttgtcg gcgcgcgcct gatggatcca gctctccagc
    12001 cgcacgccgc tccagcgcag gtcggcggtc cacagcgcct gtgcgttgcg accgtcgagc
    12061 tgcaccgtgc cgaacaggcg gccctggccg gtgcgcgcct cgagttcgcg caaggtcagc
    12121 acgccgtcga tcagcacgag gtgcgtgcgc agcggcttca ggggttcgag aaagccgctg
    12181 ccgagatcga gcgtgtcgat gtcgatcagc acgttggcgt ccatcgcgcg cagcgacggc
    12241 aggtcgaact cgcggttcgg cagcacgtgg ccaggtgcgg cacgcgcacg cggcgcggcc
    12301 ggtgtgacct cgggcacggc ggccgatttc ttcgccgtcg tgccgaacgc cgggccgagg
    12361 tcgacgagca gcagcttggt gccggtcagc cggccggcga gcaccggccg ggcgctgcgc
    12421 gggtcatagg tgaacgcgcc ggccaggcgg ctcgagccga tcgtcgcggc ctcgaccacc
    12481 gtgttccaga ccaggccctg cttcgcgatc acaccttgcg cgcgaaacgg tccggtcgtc
    12541 ggcaaggtga cgtgcagcgg gtcgccgatc gccgcgagcg aagggccttg cacgctgaag
    12601 cgaccctgca gcgcgctcag gtgcagcgca tcggtggcag tgccgcggaa cgtcacgctg
    12661 gcgccgccga tgtgcgcgtc caatgtcacc ggcagcgcct gggtcacggc atcgtcggcg
    12721 atcaccggca
//
LOCUS       DATMJB010000411         2886 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_723979, whole genome shotgun sequence.
ACCESSION   DATMJB010000411 DATMJB010000000
VERSION     DATMJB010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723979"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="VNU48_08895"
     CDS             complement(<1..172)
                     /locus_tag="VNU48_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWI11430.1"
                     /translation="MSLINTQIKPFAATAYKAGKFIDVTQDTLKGKWAVLMFYPADFT
                     FVCPTELGDLADH"
     gene            379..1821
                     /locus_tag="VNU48_08900"
     CDS             379..1821
                     /locus_tag="VNU48_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11431.1"
                     /translation="MIALLLHPWVACAEDVHAESLPTSGTLSFERVRLPQGERMGLVG
                     GSLLFDVGADWGLGPAVYGAATGRRGGLFVGGIELQHRWSLGKGLSLAAGLYAGGGGG
                     AAAPVGSGLMLRPAVTLLKDLGPTFQLGLSWSSIRFPSGDIRSNQIGLALGWRQEFVH
                     LSGAGAVPYQAGSMSGGLGFDRMVATVSTYRLTDATRRHVGLIGARAERRSDIDGFAW
                     GMEAAAAAKGDAAGYMELLGNAVLSFAPLPSALPSWRVGARLSAGVGGGGDVRTGGGL
                     LAKALGTMEWSPVAGWVVGTDYGVVRARDGVLAREAQVWLGLYLEPGMDGRPTMPARV
                     VRTEWVGAWQHHSRIARSDGSQRSLDTIGLKLNRYIGDHFYLTGQAHSAFAGGAGAYS
                     VGLIGLGVSATPAASVRVGAEALVGAAGGGGVATTGGAVTQALMWTAWQPGRVGEIRL
                     GVGASRPLHNSRTEPVVELSWSRAFGMAGS"
     gene            complement(1833..2411)
                     /locus_tag="VNU48_08905"
     CDS             complement(1833..2411)
                     /locus_tag="VNU48_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HWI11432.1"
                     /translation="MTITKDSLMTLEAYAKIRKTARAEFIAHRRLRTVHLGEHLSVQF
                     EDERTIRRQIQEMLHIEKIFDEDGINAEIEAYAALVPEGTNWKATVLIEYPDVHERKR
                     ELARLIGIEDRLFVEVEGHARVYAIADEDLERENAEKTSAVHFARFEFDKSVRDAVRA
                     GASVKLGCDHTNYPAHVAISAETLASLAGDLT"
     gene            complement(2417..>2886)
                     /locus_tag="VNU48_08910"
     CDS             complement(2417..>2886)
                     /locus_tag="VNU48_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HWI11433.1"
                     /translation="LFPDDADVKLVQQAMYDPFEYLVARHKDGLLKTDFKTPLGKISY
                     HIPCHGRVQKIGRKTEEMFKLIGQTVPVQLNTVERCSGHAGTYGVKTPYHPVAMKIGK
                     PVFKAMGKDAPDYISSDCALAGHHIAQGMAQDGLPTATLAHPLSLMRIAYGLE"
BASE COUNT          389 a          957 c         1022 g          518 t
ORIGIN      
        1 agtggtcggc caggtcgccc aattcggtcg ggcagacgaa ggtgaagtcg gccgggtaga
       61 acatcagcac cgcccacttg cccttgagcg tgtcctgggt cacgtcgatg aacttgccgg
      121 ccttgtaggc ggtggcggcg aaaggtttga tttgagtgtt gatcagagac atcgttggtt
      181 ccttgaaagt gaggttgaga actgaatcgg tacttaagaa taagctatcg actttgtttc
      241 attaattgat attgactaag taatggatag tcgcccggcc ccgtccgatc cgtcggcgcg
      301 gcgtgatcca atccacgcgc gtcattgcct gtcgattcac gcgaggcccc cttgatcccg
      361 ctgcgaaacc tgcccttctt gatcgccctg ctgctgcacc cctgggtcgc gtgcgcagag
      421 gatgtccacg ccgagtcgct gccgacgtcg ggcacgctca gcttcgaacg cgtgcgactg
      481 ccgcagggcg agcgcatggg gctggtcgga ggctcgctgc tgttcgacgt cggcgctgac
      541 tggggcctcg gtccggccgt gtacggcgcg gccacggggc ggcgcggcgg tctgttcgtc
      601 ggcggcatcg agctgcagca ccgctggagc ctgggcaaag gtctgtcgct ggccgcgggt
      661 ctctacgccg gcggcggcgg cggcgccgct gcgccggtgg gcagcggcct catgctgcgt
      721 cccgccgtca cgctgctgaa ggacctcggc ccgaccttcc agctcggcct gtcctggtcg
      781 tcgatacgat tcccgagcgg cgacatccgc agcaaccaga tcggcctggc gctcggctgg
      841 cgccaggagt tcgtgcacct gagcggcgcg ggcgcggtcc cctaccaggc cggttcgatg
      901 tcgggcggcc tcggcttcga ccgcatggtg gcgacggtct cgacctaccg gctcacggac
      961 gcgacccgac gccacgtcgg cctgatcggt gcgcgcgccg agcgccgctc cgacatcgac
     1021 ggcttcgcct ggggcatgga ggccgccgcc gccgcgaagg gcgatgcggc gggctacatg
     1081 gaactgctcg gcaacgcggt gctgagcttc gcaccgctgc cgtcggcact gccctcgtgg
     1141 cgcgtcggtg cccggctcag cgccggcgtg ggcggtggcg gcgacgtccg caccggcggc
     1201 ggcctgctcg ccaaggcgct cggcacgatg gaatggagcc cggtcgcggg ctgggtcgtc
     1261 ggtaccgatt acggcgtcgt gcgtgcgcgc gacggcgtgc tcgcgcgcga ggcgcaggtc
     1321 tggctggggc tgtacctgga gcccgggatg gatggtcgcc ccacgatgcc tgcgcgtgtc
     1381 gtgcgcaccg aatgggtcgg cgcgtggcag caccactcgc gcatcgcgcg cagcgacggc
     1441 agccagcgct cgctcgacac gatcggcctg aaactgaacc gctacatcgg cgaccacttc
     1501 tacctgaccg gccaggccca cagcgcattc gccggcggcg ccggcgcgta ctcggtcggc
     1561 ctgatcgggc tgggcgtgag cgccacaccc gcggcttcgg tgcgcgtcgg cgccgaggca
     1621 ctggtcggcg cggccggtgg gggcggtgtg gccaccaccg gaggtgccgt cacccaggcg
     1681 ctgatgtgga cggcgtggca gccgggccgc gtcggagaga tccgtctggg tgtgggcgcg
     1741 tcgcgcccgc tgcacaacag ccgcaccgag ccggtggtcg aactctcgtg gagccgcgcg
     1801 ttcgggatgg ccggaagctg aggcgactcg cgctacgtca ggtcgccggc caggctcgcc
     1861 agcgtctcgg ccgagatcgc gacgtgtgcc gggtagttcg tgtgatcgca gccgagcttg
     1921 acgctcgcgc cggcacgcac cgcgtcgcgc acggacttgt cgaactcgaa gcgcgcgaag
     1981 tgcaccgccg aggtcttctc ggcgttctcg cgctcgaggt cttcatcggc gatcgcgtag
     2041 acgcgcgcat ggccttcgac ctcgacgaag aggcggtcct cgataccgat cagccgcgcc
     2101 agctcgcgct tgcgctcgtg cacgtcgggg tactcgatca gcacggtggc cttccagttc
     2161 gtgccctcgg gcacgagcgc cgcataggcc tcgatctcgg cgttgatgcc gtcctcgtcg
     2221 aagatcttct cgatgtgcag catctcctgg atctgccggc ggatcgtgcg ctcgtcctcg
     2281 aactggacgc tgaggtgctc gcccaggtgc acggtgcgca gcctgcggtg cgcgatgaac
     2341 tcggcacgcg cggtcttgcg gatcttcgcg taggcctcca gcgtcatcag gctgtctttg
     2401 gtgatggtca ttcgcttcac tccagcccgt aggcgatgcg catcaggctc agcgggtgcg
     2461 ccagtgtcgc ggtgggcagc ccgtcctgcg ccatgccctg cgcgatgtgg tggccggcca
     2521 gcgcgcaatc ggagctgatg tagtcgggcg cgtccttgcc catcgccttg aacaccggct
     2581 tgccgatctt catcgccacc gggtggtagg gcgtcttcac gccgtaagtg ccggcgtggc
     2641 cggagcagcg ctccaccgta ttgagctgca ccggcaccgt ctgcccgatc agcttgaaca
     2701 tctcctcggt cttgcggccg atcttctgca cccggccgtg gcaggggatg tggtagctga
     2761 tcttgcccag cggcgtcttg aagtcggtct tgagcaggcc gtccttgtgg cgcgccacca
     2821 ggtactcgaa ggggtcgtac atggcctgct gcaccagctt cacatcggcg tcgtcaggga
     2881 agagca
//
LOCUS       DATMJB010000412         2104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_656347, whole genome shotgun sequence.
ACCESSION   DATMJB010000412 DATMJB010000000
VERSION     DATMJB010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656347"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="VNU48_08915"
     CDS             complement(<1..1301)
                     /locus_tag="VNU48_08915"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HWI11434.1"
                     /translation="MAVSVFDLFKIGIGPSSSHTVGPMRAARLFALRLEHEGKLAATA
                     RVCSQLYGSLGATGKGHGTDKAVLLGLTGQEPDTVDVEHIGSALKRIREARRIALLGT
                     HEIGFTEKTDLIFHRREALPFHANGMRFTAFDAAGAELLTRSYYSVGGGFVVSDEVAA
                     DGRLQKVIAPDTAVLAHPFHSGADLLLLTKQQGRSIAELMRRNERHWRTDAEIDAGLL
                     KIWAVMQACVLRGCATDGVLPGGFKVRRRAADLHRQLCANPDAAKGHEEARYGGPRDP
                     LQVLDWVNLYALAVNEENAAGGRVVTAPTNGAAGIVPAVLHYYTRFVPGATEARVIDF
                     LLTAAAIGILYKENASISGAEVGCQGEVGVACSMAAGALCAVLGGTPEQVENAAEIGM
                     EHHLGLTCDPVGGLVQIPCIERNAIASVKAINAARMALRGDG"
     gene            complement(1306..>2104)
                     /locus_tag="VNU48_08920"
     CDS             complement(1306..>2104)
                     /locus_tag="VNU48_08920"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905599.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HWI11435.1"
                     /translation="KTFCIPHGGGGPGVGPTCVVADLVPYLPAHRTAGIGSDAQVGAV
                     SAAPLGNAAVLPISWMYMRMMGSEGLTAATEVAILSANYIAARLADHYDIHFSGNVKG
                     IKGGGVAHECILDLRPLKEATGGDGSGAQGISAEDVAKRLIDYGFHAPTLSFPVAGTL
                     MVEPTESESLEELDRFCDAMIAIRGEIAKVESGAWPRDDNPLKAAPHTALALLKAEWP
                     HAYSREEAAFPVAALRRGKYWSPVGRVDNVYGDRNLFCSCPPLSSYE"
BASE COUNT          285 a          741 c          753 g          325 t
ORIGIN      
        1 ccgtcgccgc gcagcgccat gcgcgcggcg ttgatcgcct tcaccgaggc gatcgcgttg
       61 cgctcgatgc acgggatctg caccagcccg cccacgggat cgcaggtcag gcccaggtgg
      121 tgctccatgc cgatctcggc cgcgttctcg acctgttccg gcgtgccgcc cagcaccgcg
      181 cacagcgcgc cggcggccat cgagcaggcg acgccgacct cgccctggca gccgacctcg
      241 gcgccgctga tcgacgcgtt ctccttgtag aggatgccga tcgcggcggc cgtgagcagg
      301 aagtcgatca cgcgcgcctc ggtggcgccg ggcacgaagc gcgtgtagta gtgcagcacg
      361 gcgggcacga tgccggcggc gccgttcgtc ggtgcggtga cgacgcggcc gccggcggcg
      421 ttctcctcgt tgaccgcgag cgcgtagaga ttcacccagt cgagcacctg cagcgggtcg
      481 cgaggaccgc catagcgggc ctcttcgtgg cccttcgccg cgtcggggtt cgcgcacagc
      541 tggcggtgca gatcggcggc gcgccgcctg accttgaagc cgccgggcag cacgccgtcg
      601 gtggcgcagc cgcgcagcac gcaggcctgc atcacggccc agatcttcag cagcccggcg
      661 tcgatctcgg cgtccgtccg ccagtgacgc tcgttgcgcc gcatcagctc ggcgatgctg
      721 cggccctgct gcttcgtcag cagcagcaga tcggcgccgc tgtggaaggg gtgcgcgagc
      781 acggcggtgt cgggcgcgat caccttctgc aggcggccgt cggcggcgac ctcgtcgctg
      841 acgacgaagc cgccgccgac cgagtaatag ctgcgcgtca gcagctccgc tccggccgca
      901 tcgaacgcgg tgaagcgcat gccgttcgcg tggaagggca gggcctcgcg gcggtggaag
      961 atgaggtcgg tcttctcggt gaacccgatc tcgtgcgtgc cgagcagcgc gatgcgccgg
     1021 gcctcgcgga tgcgcttgag cgcgctgccg atgtgctcga cgtcgacggt gtcgggttcc
     1081 tggcccgtca ggcccagcaa caccgccttg tcggtgccgt ggcccttgcc ggtggcaccg
     1141 agcgagccgt acagctgcga gcacacgcgc gccgtcgccg cgagcttgcc ctcgtgttcg
     1201 aggcgcagcg cgaacaggcg tgctgcgcgc atggggccga ccgtgtgcga gctgctgggg
     1261 ccgatgccga tcttgaacag gtcgaagacc gagacggcca ttggcttact cgtagctgct
     1321 cagcggcggg caggaacaga acaggttgcg gtcgccgtag acgttgtcga cccggcccac
     1381 cggcgaccag tacttgccgc ggcgcagcgc cgccaccggg aaggcggcct cttcgcgcga
     1441 gtaggcgtgg ggccactcgg ccttcaacag cgccagcgcg gtgtgcggcg cggccttcag
     1501 cgggttgtcg tcgcgcggcc aggcccccga ctcgaccttc gcgatctcgc cgcggatcgc
     1561 gatcatcgcg tcgcagaagc ggtccagctc ctccagcgat tcgctctcgg tcggttcgac
     1621 catcaaggtg ccggcgaccg gaaagctcag cgtcggcgcg tggaagccgt agtcgatcag
     1681 gcgcttggcg acgtcctcgg cgctgatgcc ctgggcgccc gatccatcgc cgccggtcgc
     1741 ctccttcaag ggccgcaggt cgagaatgca ctcgtgcgcg acgccgccgc ccttgatgcc
     1801 tttgacgttg ccgctgaagt ggatgtcgta gtgatcggcc aggcgtgccg cgatgtagtt
     1861 cgccgacagg atcgccacct cggtcgccgc ggtcaggccc tcgctgccca tcatgcgcat
     1921 gtacatccag ctgatcggca gcaccgcggc attgcccagc ggggccgccg agacggcgcc
     1981 gacctgtgcg tcacttccga tgccggcggt gcgatgcgcg ggcaggtacg gcaccaggtc
     2041 ggccacgacg caggtcggcc cgactccggg cccgccgccg ccgtgcggga tgcagaaggt
     2101 cttg
//
LOCUS       DATMJB010000413         4048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_240651, whole genome shotgun sequence.
ACCESSION   DATMJB010000413 DATMJB010000000
VERSION     DATMJB010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240651"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1095
                     /locus_tag="VNU48_08925"
     CDS             67..1095
                     /locus_tag="VNU48_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWI11436.1"
                     /translation="MIIDVHGHYTTAPKALEAWRNRQIAGIKDPALSPKVSELKISDD
                     ELRESIETNQLAKMRERGSDLTIFSPRASFMAHHIGDFEVSSTWSAICNELCYRVSKL
                     FPDHFIPAAMLPQSPGVDPATCIPELVKCVEQFGNVGINLNPDPSGGHWTSPPLSDRH
                     WYPIYEKMVEYDIPAMVHVSTSCNACFHTTGAHYLNADTTAFMQCLTSDLFKDFPTLR
                     FIIPHGGGAVPYHWGRFRGLAQELKKPLLEDHLLNNIFFDTCVYHQPGIDLLTKVIPV
                     KNILFASEMIGAVRGIDPETGHYYDDTKRYIEASTIVAGDDRHRIYEGNARRVFPRLD
                     AALKAKGK"
     gene            1113..1796
                     /gene="ligK"
                     /locus_tag="VNU48_08930"
     CDS             1113..1796
                     /gene="ligK"
                     /locus_tag="VNU48_08930"
                     /EC_number="4.1.1.112"
                     /EC_number="4.1.3.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HWI11437.1"
                     /translation="MNNLGIVKRGIVRADRAAVEKLSRFGVATIHEAMGRAGLMKTYM
                     RPVYTGAHLCGTAVTVLLQPGDNWMMHVAAEQLQPGDVAVAACTADNTDGFFGDLLAT
                     SFKARGARGLVIDGGCRDVKELQEMGFPVFSRAISAKGTIKATLGSVNVPVVCAGALV
                     NPGDVVIADADGVVVVPAAIARQVADAAEKRESFEGEKRAKLAAGVLGLDMYKMREPL
                     EKAGLKYID"
     gene            1810..2733
                     /locus_tag="VNU48_08935"
     CDS             1810..2733
                     /locus_tag="VNU48_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HWI11438.1"
                     /translation="MPDPLHWQVGLVGYGEVGRILAEDLRRRDVRVGAFDLKLDDADA
                     ARALRDHASAHGVTLAASPAALAGRSDLIVSAVTASQAVPVAQACAPAVRRDTFFLDF
                     NSASPGAKIRAAALIDAAGGRYVEGAVMTSIPPYRIKVPLLLGGAHAAALAPLLELLG
                     FAPKVASERLGVASATKMCRSVMIKGLEAMVIESYTAARAYGVEDAVLASLNETFPGI
                     DWEKQGAYFFQRVIAHGRRRAEEVREVAETVRDIGLTPWSAAGTADRQAWIADLADAG
                     LFGARGGADFARSADWRIEADRILAKVRPPR"
     gene            2751..3665
                     /locus_tag="VNU48_08940"
     CDS             2751..3665
                     /locus_tag="VNU48_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWI11439.1"
                     /translation="MEFTKTPGWLDWYAGPSTPAFRLPAGAVDAHCHVFGPGAEFPYA
                     PQRKYTPCDASKQQLYALRDHLGFARNVVVQATCHGADNRAMVDALLHSGGKARGVAT
                     VRRSVTDRELQDMHAAGVRGVRFNFVKRLVDFTPKDELLEIAVRIAPLGWHVVIYFEA
                     VDLPELWDFFTALPTTVVVDHMGRPDVSLPVDGAQFELFVRLMREHENVWSKVSCPER
                     LSVTGPKALNGERHAYRDVVPFARRIVDSFPDRVLWGTDWPHPNLKDHMPDDGLLVDF
                     IPHIATTPELQRKLLVDNPMRLYWPEER"
     gene            3665..>4048
                     /gene="ligA"
                     /locus_tag="VNU48_08945"
     CDS             3665..>4048
                     /gene="ligA"
                     /locus_tag="VNU48_08945"
                     /EC_number="1.13.11.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018579 - protocatechuate 4,5-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 4,5-dioxygenase subunit alpha"
                     /protein_id="HWI11440.1"
                     /translation="MSLDKPYLDVPGTTIFDAEQSRKGYHLNQFCMSLMKAENRARFK
                     ADERAYLDEWAMTEEQKQAVLARDLNWCIRLGGNIYFLAKIGATDGKSFQQMAGSMTG
                     MSEEEYKDMMIHGGRSIEGNRVVGED"
BASE COUNT          696 a         1401 c         1353 g          598 t
ORIGIN      
        1 catgagaaag ctgcgccagg cacggcgcgc ggtcgacaat cccgtggctg ccaggagagc
       61 ccttccatga tcatcgacgt ccacggccac tacaccaccg ccccgaaggc gctcgaggcg
      121 tggcgcaacc ggcagatcgc cggcatcaag gacccggcgc tcagccccaa ggtcagcgag
      181 ctgaagatca gcgacgacga actgcgcgaa tcgatcgaga ccaaccagct cgcgaagatg
      241 cgcgagcgcg gcagcgacct gacgatcttc agcccgcgcg ccagcttcat ggcgcaccac
      301 atcggcgact tcgaggtcag ttcgacctgg tcggcgatct gcaacgagct gtgctaccgg
      361 gtgtcgaagc tcttccccga ccacttcatc ccggcggcga tgttgccgca gtcccccggg
      421 gtcgatccgg cgacctgcat tcccgaactc gtcaagtgcg tcgagcagtt cggcaacgtg
      481 ggcatcaacc tgaaccccga tccgtccggc ggccactgga cctcgccgcc gctgtcggac
      541 cgccactggt acccgatcta cgagaagatg gtcgagtacg acatcccggc catggtgcac
      601 gtcagcacga gctgcaatgc gtgcttccac accaccggcg cgcactacct gaacgccgac
      661 acgacggcct tcatgcagtg cctcacgtcc gacctgttca aggactttcc gaccctgcgc
      721 ttcatcatcc cgcacggcgg cggcgcggtg ccctaccact gggggcgctt ccgcggcctc
      781 gcgcaggagc tgaagaagcc gctcctcgag gaccacctgc tcaacaacat cttcttcgac
      841 acctgcgtct accatcagcc cggcatcgac ctgctgacca aggtcattcc ggtcaagaac
      901 atcctgttcg cgagcgagat gatcggcgcg gtgcgcggca tcgatcccga gaccggccac
      961 tactacgacg acaccaagcg ctacatcgag gcctcgacga tcgtcgccgg cgacgaccgc
     1021 caccggatct acgaaggcaa cgcccggcgc gtgttcccgc gcctggacgc ggcactcaaa
     1081 gcgaaaggca agtgatgacc atggacctgc cgatgaacaa cctggggatc gtcaagcgcg
     1141 gcatcgtgcg cgccgaccgc gccgcggtcg agaagctgtc gcgcttcggc gtcgcgacca
     1201 tccacgaagc gatgggccgg gccggcttga tgaagaccta catgcggccg gtctacaccg
     1261 gcgcgcatct ttgcggcacg gccgtcaccg tactgctgca gccgggcgac aactggatga
     1321 tgcacgtcgc cgccgagcag ctgcagcccg gcgacgtggc ggtggcggcc tgcacggcgg
     1381 acaacaccga cggcttcttc ggcgacctgc tcgccacgtc gttcaaggcg cgcggcgcgc
     1441 gtggcctggt catcgacggc ggctgccgcg acgtgaagga actgcaggag atgggcttcc
     1501 cggtcttcag ccgggcgatc agcgcgaagg gcacgatcaa ggccacgctc ggctcggtga
     1561 acgtgccggt ggtgtgcgcg ggtgcgctgg tgaatccggg cgacgtggtg atcgccgatg
     1621 ccgacggcgt cgtcgtggtg cccgccgcga tcgcccggca ggtcgccgat gccgccgaga
     1681 agcgcgagtc gttcgagggc gagaagcgcg ccaagctcgc cgccggcgtg ctggggctgg
     1741 acatgtacaa gatgcgcgag ccgctcgaga aggccggcct caagtacatc gactgacggg
     1801 ctggcaccga tgcctgatcc actgcactgg caggtcggcc tcgtcggcta cggcgaggtg
     1861 ggccgcatcc ttgccgaaga cctgcgccgg cgcgacgtgc gggttggcgc gttcgatctg
     1921 aagctcgacg acgccgacgc ggcccgggca ctgcgcgatc acgcgtcggc gcatggggtc
     1981 acgctggcgg cgagccctgc cgcgctggcc ggtcgatccg acctgatcgt ctcggcggtg
     2041 accgcgagcc aggccgttcc ggtggcgcag gcctgcgccc cggcggtgcg acgcgacacc
     2101 ttcttcctcg acttcaactc ggcttcgccg ggcgcgaaga tccgcgccgc cgcactgatc
     2161 gacgcggccg gcgggcgcta cgtcgagggc gcggtgatga cgtcgatccc gccgtaccgc
     2221 atcaaggtcc ctttgctgct cggcggcgcg cacgccgcgg cgctggcgcc gctgctcgag
     2281 ctgctcggct tcgccccgaa ggtcgcgagc gaacggctcg gcgtcgcgtc ggcgacgaag
     2341 atgtgccgca gcgtgatgat caagggtctg gaggcgatgg tcatcgagag ctacaccgcg
     2401 gcgcgcgcct acggggtcga ggatgcggtg ctcgcgtcgc tgaacgagac cttcccgggc
     2461 atcgattggg agaagcaggg cgcctacttc ttccagcgtg tgatcgcgca cggccggcgc
     2521 cgtgccgagg aagtgcgcga agtcgccgag accgtgcgcg acatcgggct gacaccgtgg
     2581 tcggccgcgg gcaccgcgga ccgccaggcc tggatcgccg acctcgccga cgcgggcctg
     2641 ttcggggcgc gcggcggcgc ggatttcgcc cgcagcgccg actggcgcat cgaggccgat
     2701 cggatcctcg ccaaggtcag gccgccgcgc tgacgacagg aacgaccacg atggaattca
     2761 ccaagacccc cggctggctc gactggtacg ccggcccctc gacaccggcc ttccggctgc
     2821 cggccggcgc ggtcgacgcg cattgccatg tgttcgggcc cggcgccgaa ttcccgtacg
     2881 cgccgcagcg caagtacacg ccgtgcgacg cgtcaaagca gcagctctat gcactgcgcg
     2941 accacctcgg cttcgcgcgc aacgtggtcg tgcaggcgac gtgccacggc gccgacaacc
     3001 gcgcgatggt cgatgcgctg ctgcacagcg gcggcaaggc gcgcggcgtc gccaccgtca
     3061 ggcgcagcgt caccgatcgc gagctgcagg acatgcacgc cgccggtgtg cgcggcgtgc
     3121 gcttcaactt cgtcaagcgg ctggtcgact tcacgccgaa ggacgagttg ctggagatcg
     3181 ccgtgcggat cgccccgctc ggctggcatg tcgtgatcta tttcgaggcc gtcgacctgc
     3241 ccgagctgtg ggacttcttc accgcgctgc cgacgaccgt cgtcgtcgat cacatgggcc
     3301 ggcccgatgt gagcctgcca gtcgacgggg cgcagttcga gttgttcgtc aggctcatgc
     3361 gcgagcacga aaacgtgtgg agcaaggtca gctgcccgga gcgcctgtcc gtcaccggcc
     3421 cgaaggcgct gaacggcgaa cgccatgcgt accgcgacgt ggtgccgttc gcccggcgca
     3481 tcgtcgacag cttccccgac cgcgtgctgt ggggcaccga ctggccgcac ccgaacctga
     3541 aggatcacat gcccgacgac gggctgctgg tcgacttcat cccgcacatc gcgacgacgc
     3601 ccgagctgca gcgcaagctg ctggtcgaca acccgatgcg cctgtactgg cctgaggaac
     3661 gctgatgtca ctcgataaac cgtacctgga cgtgcccggc acgaccatct tcgacgccga
     3721 acagtcgcgc aagggctacc acctgaacca gttctgcatg tcgctgatga aggccgagaa
     3781 ccgcgcgcgc ttcaaggccg acgaacgcgc ctacctggac gaatgggcga tgaccgaaga
     3841 gcagaagcag gctgtgctcg cgagagacct caactggtgc atccgcctgg gcggcaacat
     3901 ctacttcctg gccaagatcg gcgccaccga cggcaagagc tttcagcaga tggccggcag
     3961 catgaccgga atgagcgaag aggaatacaa ggacatgatg atccacggcg gccgctcgat
     4021 cgaaggcaac cgcgtcgtcg gcgaagac
//
LOCUS       DATMJB010000414         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_443444, whole genome shotgun sequence.
ACCESSION   DATMJB010000414 DATMJB010000000
VERSION     DATMJB010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443444"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1132)
                     /gene="yidC"
                     /locus_tag="VNU48_08950"
     CDS             complement(<1..1132)
                     /gene="yidC"
                     /locus_tag="VNU48_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HWI11441.1"
                     /translation="MTDMRRTLLWVVFSMSLFLIYDAWNKYNGHPSFFSPAPAKVAAA
                     GSAPASGALPTPAGAIAPPTASGSQALAAPVAAEQLTITTDVVKASLSSAGGTLTRVE
                     LLKYPEQVLKEWYEPLLDMVGKGQAAAAPAKDVVLLDESPERLYVAESGLIPAAGGSG
                     LPNHHTLMTPVPGERVLAAGQNALQVRFESPAVGGVKLVKTYTFRRGDYVIDVKNEVV
                     NESGAPVSPRLYLQLVRDGVPPPGGSSVYSTFTGPAIDDGNKFQKIDFKEIDKHAPTD
                     KPYHTIDADTGWIAMVQHYFTSAWLIDKADGTRQPREFFTGKSEMVNPSTGAPVHVYS
                     VGMFVPIGEVAPGATKTFDAKLFVGPQEENKLAALAPRLELVK"
     gene            complement(1129..1434)
                     /gene="yidD"
                     /locus_tag="VNU48_08955"
     CDS             complement(1129..1434)
                     /gene="yidD"
                     /locus_tag="VNU48_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HWI11442.1"
                     /translation="MPQRLLIASVRAYRLLLSPWLGSSCRFEPTCSAYALQALQQHGA
                     TAGTYLTLRRLARCQPFCAGGHDPVPEGAPALFRRFLAASPRADGFLSPSSIDKNSP"
     gene            complement(1471..1920)
                     /locus_tag="VNU48_08960"
     CDS             complement(1471..1920)
                     /locus_tag="VNU48_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HWI11443.1"
                     /translation="MRSADFERVLRTRSRASSPHFALHHVAARPSTPAKPLVADLSTG
                     EVSADRPPVDDWPAPALGALWLGAVVPKRHARRSVTRSLLKRQIRAVVGDHAAALAGG
                     LWVVRLRAPFDRARFSSAASAELKRAARAELEGLVCSAAAPRVVSGA"
     gene            complement(1962..2096)
                     /gene="rpmH"
                     /locus_tag="VNU48_08965"
     CDS             complement(1962..2096)
                     /gene="rpmH"
                     /locus_tag="VNU48_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HWI11444.1"
                     /translation="MKRTYQASKVRRARTHGFLVRMKSRGGRAVINARRAKGRKRLAV
                     "
BASE COUNT          362 a          764 c          801 g          342 t
ORIGIN      
        1 ccttgacgag ctcgaggcgc ggcgcgagcg cggccagctt gttctcttcc tgcgggccga
       61 cgaacagctt ggcgtcgaag gtcttggtcg cgccgggggc gacctcgccg atcggcacga
      121 acatcccgac cgagtagaca tgcaccggcg cgccggtcga cgggttgacc atctccgact
      181 tgccggtgaa gaactcgcgc ggctgcctgg tgccgtcggc cttgtcgatc agccacgccg
      241 aggtgaagta gtgctggacc atggcgatcc agccggtgtc ggcgtcgatc gtgtggtacg
      301 gcttgtccgt cggcgcgtgc ttgtcgatct ccttgaaatc gatcttctgg aacttgttgc
      361 cgtcgtcgat cgccgggccg gtgaaggtgg aatagacgct cgacccgccc ggcggcggca
      421 cgccgtcgcg caccagctgc aggtacaggc gcggcgacac cggcgcgccc gattcgttga
      481 cgacctcgtt cttcacgtcg atcacgtagt cgccgcgcct gaaggtgtag gtcttgacca
      541 gcttgacgcc gccgaccgcc ggcgattcga acctcacctg caacgcgttc tggcccgctg
      601 ccagcacgcg ctcgcccggc acgggtgtca tcagcgtgtg gtggttcggc aggccggaac
      661 cgcccgccgc cgggatcagg cccgactcgg ccacatacag gcgctcgggg gattcgtcga
      721 gcagcaccac gtccttcgcc ggcgctgccg cggcctgacc cttgccgacc atgtcgagca
      781 aaggctcgta ccactccttc aggacctgct cgggatattt gagcagttcg acgcgcgtca
      841 gcgtgccgcc ggcgctgctg aggctggcct tgacgacgtc ggtggtgatg gtcagctgtt
      901 ccgccgccac gggcgccgcc agcgcctggc tgcccgacgc ggtcggcggg gcgatggcgc
      961 ccgcgggcgt cggcagcgca cccgacgccg gggccgaccc ggccgccgcc accttcgccg
     1021 gcgcggggct gaagaacgac gggtggccgt tgtacttgtt ccacgcgtcg tagatcagga
     1081 acagggacat ggaaaaaacc acccacaaca gggtgcgacg catatcggtc atggggagtt
     1141 cttgtcgatc gaagagggag agaggaatcc gtcggcacgc ggcgacgccg cgaggaaacg
     1201 gcggaacagg gccggcgccc cttcgggcac cggatcatgc ccgccggcgc aaaacggctg
     1261 gcagcgcgcc aagcggcgca atgtcaagta agtacccgct gttgccccgt gctgctgcag
     1321 cgcttgcagg gcataggccg agcaggttgg ctcgaaacga cagctgctgc ccagccacgg
     1381 actcaagagc aggcggtagg cgcgcaccga cgcaatcagc aggcgctgcg gcaaggtgcg
     1441 cagagacagc ggcatggttg ggcgagccgc tcaggcgccg ctgaccacgc gtggcgcggc
     1501 ggcactgcag accaggcctt caagttcggc gcgtgcggcc cgtttcagct cggcggacgc
     1561 tgcgctgctg aagcgcgccc tgtcgaaggg cgcgcgcagg cgcaccaccc acagtccgcc
     1621 ggccaacgca gcggcgtgat ccccgaccac ggcgcggatc tggcgcttga gcaggctgcg
     1681 cgtcaccgag cggcgcgcgt gccgcttggg cacgacggcg cccagccaga gcgcgccgag
     1741 ggctggtgca ggccagtcat ccacaggcgg cctgtctgcc gagacttcgc ctgttgacaa
     1801 gtcggcaaca agcggcttgg cgggcgtgct cgggcgggca gccacgtggt gcagcgcgaa
     1861 gtgcgggctg ctcgcccggc tgcgcgtgcg cagcacgcgc tcgaagtcgg cggaccgcac
     1921 gaggcgcgcg atcacggttc gaatgccgag gcgggcgcgc ctcagacagc cagacgcttg
     1981 cggcccttgg cgcggcgcgc gttgatcacg gcacgaccgc cgcggctctt catgcggacg
     2041 aggaagccgt gggtgcgggc gcggcgaact ttggaagctt ggtaggtgcg tttcatgatc
     2101 tgtccaactg agagcggccc ggctaccgac gcgatggcct cgttcgaagc acacccacga
     2161 acctgacggt gccggggtca cgcctggggc cgatgggctc ggctccggga aacccactat
     2221 tacaccaaaa accccctgtt cggtcaatga cttacaccac cggccgtag
//
LOCUS       DATMJB010000415         2269 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_306703, whole genome shotgun sequence.
ACCESSION   DATMJB010000415 DATMJB010000000
VERSION     DATMJB010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306703"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VNU48_08970"
     CDS             complement(<1..494)
                     /locus_tag="VNU48_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HWI11445.1"
                     /translation="MLFGKLLPREGNFFELFNQHGDFIVAGARAFILLIQNYADPQLR
                     DKYAEEVGTAERQADRITAEVNRLLHKTFITPIDREQIHSLINAMDDILDLLQDATET
                     MSLYDVRAMTEDVLRLGDLSARCCERVQHAVSLLPKINQAEAAEAAIKTCEEIDKLES
                     DADRV"
     gene            complement(599..1267)
                     /locus_tag="VNU48_08975"
     CDS             complement(599..1267)
                     /locus_tag="VNU48_08975"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HWI11446.1"
                     /translation="MATTPGLERFRHIAIEGPIGVGKSTLARKLAAHLGADTLLEQPL
                     ENPFLSRFYEDMPGYAFQTQLAFLFQRVKQMHAAAQPGMFTSAVVSDFLFAKDALFAR
                     LNLSDDEFRLYGQMYAQVAPQVREPDLVVWLQAPPATLLQRIHRRAIPMEQGIAPDYL
                     QRLCDAYVEYFHAYDGAPVFALGTERFNPVDRDADFAVLLERLAAFRGRREFFNSHVE
                     IPFH"
     gene            complement(1267..1620)
                     /locus_tag="VNU48_08980"
     CDS             complement(1267..1620)
                     /locus_tag="VNU48_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HWI11447.1"
                     /translation="MNAISPSLQTVLLLAASNVFMTFAWYGHLKNLADKPWYLAALVS
                     WGVALFEYLLQVPGNRIGFGAGISLAQLKITQEVITLAVFVPFAMVYMEQPFRLDFVW
                     AGLCMVGAVYFIFRG"
     gene            complement(1617..2108)
                     /gene="folK"
                     /locus_tag="VNU48_08985"
     CDS             complement(1617..2108)
                     /gene="folK"
                     /locus_tag="VNU48_08985"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWI11448.1"
                     /translation="MRRSSETAFVGLGANLGDARAALDAAFVALATLPDTQLRAASSL
                     YRTAPIDSSGPDYLNAVVRLDTRLAPRVLLGALRRIERTHGRERPYRNAPRTLDLDLL
                     LYGDRRIATTLLTVPHPRLHERAFVLHPLAEIAPHALIPGLGAIAGLLPRVAGQRIDK
                     LGP"
BASE COUNT          344 a          760 c          812 g          353 t
ORIGIN      
        1 acgcggtcgg cgtcggactc gagcttgtcg atctcttcgc aggtcttgat cgccgcttcg
       61 gcggcctcgg cctggttgat cttcggcagc agcgagacgg catgctggac ccgctcgcag
      121 caacgcgcgc tcaggtcgcc gaggcgaagc acgtcttcgg tcatcgcccg cacgtcgtac
      181 agcgacatcg tctcggtcgc gtcctgcagc agatcgagga tgtcgtccat cgcgttgatc
      241 aggctgtgga tctgctcgcg gtcgatcggc gtgatgaagg tcttgtgcag cagccggttc
      301 acctcggcgg tgatgcggtc ggcctggcgc tcggcggtgc cgacttcctc ggcgtacttg
      361 tcgcgcagtt gcgggtcggc gtagttctgg atcagcagga tgaacgcgcg cgcgcccgcg
      421 acgatgaagt cgccgtgctg gttgaacagc tcgaagaaat tgccttcacg cggcagcagc
      481 ttgccgaaca gcatcggtgg gttcccctgt ctctgcctga atcccgcgaa agtcacggaa
      541 ccgtcatcaa atcgactcgg cgggagtgta cttgcgcgct cgcgcggcca cgcgccgttc
      601 agtggaacgg gatctccacg tgcgagttga agaactcgcg gcggccgcgg aacgccgcca
      661 ggcgctccag cagcaccgcg aaatcggcgt cgcgatcgac cgggttgaag cgctcggtgc
      721 cgagcgcgaa caccggcgcg ccatcgtagg cgtgaaagta ttcgacgtag gcatcgcaca
      781 gccgctgcag gtagtcgggt gcgatgccct gctccatcgg gatcgcgcgc cggtggatgc
      841 gctgcagcag cgtcgccggc ggcgcctgca gccacaccac caggtcgggc tcgcgcacct
      901 gcggcgcgac ctgcgcgtac atctgtccgt acaggcggaa ctcgtcgtcg ctgaggttca
      961 gccgcgcgaa cagcgcgtcc ttggcgaaca ggaagtcgct gaccacggcg ctggtgaaca
     1021 tgccgggctg cgccgccgcg tgcatctgct tgacgcgctg gaacaggaac gcgagctgcg
     1081 tctggaacgc atagcccggc atgtcctcgt agaagcggct gaggaacggg ttctcgagcg
     1141 gctgttccag cagcgtgtcg gcacccaggt gcgcggcgag cttgcgcgcc agcgtgctct
     1201 tgccgacgcc gatcgggccc tcgatcgcga tgtggcggaa gcgctcgagg ccaggcgtcg
     1261 ttgccatcag ccgcggaaga tgaagtagac cgcgccgacc atgcacaggc cggcccagac
     1321 gaagtcgagc ctgaacggct gctccatgta gaccatcgcg aacggcacga acaccgcgag
     1381 cgtgatcacc tcctgcgtga tcttcagctg cgccagcgag atgccggcgc cgaagccgat
     1441 gcggttgccc ggcacctgca gcaggtattc gaacagcgcg acgccccagc tgaccagcgc
     1501 cgccaggtac cagggtttgt cggccaggtt cttcaggtgg ccgtaccacg cgaacgtcat
     1561 gaagacgttc gacgcggcca gcagcagcac cgtttgcagc gacggggaga tcgcgttcat
     1621 ggcccgagtt tatcgatgcg ctgaccggcg acgcgcggca gcaggccggc gatcgcccca
     1681 agccccggga tcagtgcgtg cggcgcgatc tcggccagcg gatgcagcac gaacgcgcgc
     1741 tcgtgcaggc gcgggtgcgg cacggtcagc agcgtcgtcg cgatgcgccg gtcgccgtac
     1801 agcagcaggt ccaggtccag cgtgcgcggc gcgttgcggt acgggcgctc gcggccgtgc
     1861 gtgcgctcga tgcgtcgcag cgcgccgagc agcacgcgcg gcgcgagccg cgtgtcgagc
     1921 cgcacgaccg cgttcaggta gtccgggccg ctggagtcga tcggcgcggt gcggtagagc
     1981 gacgacgcgg cgcgcagctg cgtgtcgggc agcgtcgcca gcgcgacgaa ggccgcgtcg
     2041 agcgccgcac gcgcgtcgcc gaggttcgcg ccgagcccga cgaacgccgt ctcgctcgat
     2101 cgccgcacgg acgggcctgg cggatcagat gtcgcgcgac gggttggcgg cgatcgggtc
     2161 gaccgccgcc gtgctctcgc ccgcttcgcc cgcggcggcc ggccggcgtc ggcgccggcg
     2221 ccgcttgcgt gccggttcgg ccccgccggc cgctgcgtcg tcggcagat
//
LOCUS       DATMJB010000416         2630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_301279, whole genome shotgun sequence.
ACCESSION   DATMJB010000416 DATMJB010000000
VERSION     DATMJB010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301279"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VNU48_08990"
     CDS             complement(<1..989)
                     /locus_tag="VNU48_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWI11449.1"
                     /translation="MPSTPATSTLPGAAPHRRARFAVRGLRSAGFGVAIGLLLTGGFG
                     TPLWPNLVQSVCIATMCWFAIDLGRIPLAIWKHRRDPPDSPDAASHWPGWPLMVVALV
                     VGTVIGFSVGNELAGLILGRELPGYLRGSWRQALALLLGSLVPGTAITYWFYSRETIA
                     AKEAAVQTAQRQAAEHQLKLLESQLEPHMLFNTLANLRVLIGVDPARAQAMLDQLIAF
                     LRATLAGSRAAQHPLRAEFARLADYLALMQVRMGARLRTRFELPEALAELPVPPLLLQ
                     PLVENCIKHGLEPAVAGGRIEISAAREGDALVLRVRDTGAGLGGGAGGGTEFGL"
     gene            complement(1028..1543)
                     /locus_tag="VNU48_08995"
     CDS             complement(1028..1543)
                     /locus_tag="VNU48_08995"
                     /inference="COORDINATES: protein motif:HMM:NF042671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6622 family protein"
                     /protein_id="HWI11450.1"
                     /translation="MSIAILQHTPAWVWAVFVALIAYGLTQTRPREMGLLRVTLLPLA
                     MIALSLSGVFRAFGHAPLALGGWAAGVGAALSFARNLVEARGARWLAASGTIHVPGSW
                     LPLALIVGLFAVKYVAGAGLAIRPALAADPLFAGLCGLGYGSFGGLLLARGWGLRRLA
                     DRSQALQTART"
     gene            1763..>2630
                     /locus_tag="VNU48_09000"
     CDS             1763..>2630
                     /locus_tag="VNU48_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HWI11451.1"
                     /translation="MIGRALPALAPLLLWACSSVSLVEPRPPLPAPLPSASRPAAAAP
                     LALPSAAASPVLPAASAPPPQIESAAVAARFPDPPFSYRTPAFEPGHTSFTSNAELRT
                     ILHGLARDDDGRAGATSIRLLQVGSSQTGVPLEALQFSRAPVPLAATGATAQAARPTV
                     LLIGQQHGDEPAGAEALIVVAQELAGGALAPVLDRIDVVILARANPDGAAAGTRLTAS
                     GIDANRDHLLLRTPEAQAVAQLAREFDPAVVVDAHEYAAIGRYPQKFGAVQRFDVLIQ
                     YAMTANLPEFVTK"
BASE COUNT          382 a          977 c          970 g          301 t
ORIGIN      
        1 aggccgaact cggtgccgcc gccggcgccg ccgcccaggc cggcgccggt gtcgcgcacg
       61 cgcagcacga gcgcgtcgcc ctcgcgcgcc gcgctgatct cgatgcgccc gccggccacc
      121 gccggctcca gcccatgctt gatgcagttc tcgaccagcg gctgcagcag cagcggcggc
      181 accggcagct cggcgagcgc ctcgggcagc tcgaagcgcg tgcgcagccg cgcgcccatg
      241 cgcacctgca tcagcgcgag gtagtcggcc agccgggcga actcggcgcg cagcgggtgc
      301 tgcgcggcgc gcgagccggc cagcgtggcg cgcaggaacg cgatcagctg gtccagcatc
      361 gcctgcgcgc gtgccgggtc gacaccgatc agcacgcgca ggttcgccag cgtgttgaac
      421 agcatgtgcg gctcgagctg cgattcgagc agcttgagct ggtgctcggc ggcctggcgc
      481 tgtgccgtct gcaccgcggc ctccttcgcg gcgatggtct cgcgcgaata gaaccagtag
      541 gtgatcgccg tgcccggcac cagcgagccg agcagcagcg cgagcgcctg gcgccagctg
      601 ccgcgcagat agccgggcag ctcgcgcccg aggatcaggc cggcgagctc gttgccgacg
      661 ctgaagccga tcaccgtgcc gaccaccagc gccaccacca tcagcggcca gcccggccag
      721 tggctggccg catcgggcga gtcgggcggg tcgcggcggt gcttccagat cgcaagcgga
      781 atgcggccga ggtcgatcgc gaaccagcac atcgtcgcga tgcacaccga ctgcaccagg
      841 ttcggccaca gcggcgtgcc gaagccgccg gtcagcagca gcccgatcgc cacgccgaag
      901 ccggcactgc gcaggccgcg caccgcgaag cgcgcgcgcc ggtgcggcgc ggcgccaggc
      961 agggtggagg tggcgggtgt cgacggcatc gcggccgatt ctcccgcagc ggtgggcggc
     1021 gcgcggttca tgttcgggcg gtctgcagtg cctgcgatcg gtcggcgagt cgacgcagcc
     1081 cccagccacg ggccaggagc agcccgccga agctgccgta gccgaggccg cacaggccgg
     1141 cgaagagcgg gtcggcggcg agcgcgggcc gaatcgccag acccgcgccg gcgacgtact
     1201 tgaccgcgaa cagaccgacg atcagcgcca gcggcaacca gctgccgggc acgtggatgg
     1261 tgccgctggc cgcgagccag cgcgcgccgc gcgcctcgac cagattgcgc gcgaagctga
     1321 gcgccgcgcc gaccccggcg gcccagccgc ccagcgcgag cggcgcgtgt ccgaacgctc
     1381 tgaacacgcc gctcagcgac agcgcgatca tcgccagcgg cagcagcgtg acgcgcagca
     1441 gccccatctc gcgcgggcgg gtctgcgtca agccgtacgc gatcaaggcg acgaacacgg
     1501 cccagaccca ggcgggcgtg tgttgaagaa tggcgatgga catggtgctc ctttcagggt
     1561 tcgagacagg cgcgtggcgc cgacgtgccg cactgtgccg agcgacctcg ccgcgggcca
     1621 cggcgctgcg acgaaacggc cgatcgcgtc gcgaaacggc gcccggcggc gccgggcgcg
     1681 tgctgcaaaa tgatccgaac cccgcgcgca ggcgcgaaca cgagctcagg agcgaaggat
     1741 gcgatcgtcg ggcgcgaatc gtttgatcgg acgggcgctg ccggcgctcg cgccgctgct
     1801 gctctgggcc tgcagcagcg tctcgctggt cgaaccgagg ccgccgctgc cggcgccact
     1861 gccttcggcg tcgcggccgg ccgcggcagc gccgctcgcg ttgccgtcgg cggcggcgag
     1921 cccggtgttg ccggcagcat cggcgccgcc gccgcagatc gagagcgccg cggtcgccgc
     1981 gcgctttccc gatccgccgt tcagctaccg cacaccggcc ttcgagcctg ggcacaccag
     2041 cttcaccagc aacgccgaac tgcgcacgat cctgcacggt ctggcgcgcg acgacgacgg
     2101 ccgcgccggc gcgaccagca tccggttgct gcaggtcggc agctcgcaga ccggcgtgcc
     2161 gctcgaggcg ctgcagttca gccgcgcgcc ggtgccgctg gccgcgaccg gcgccaccgc
     2221 acaggcggcg cgcccgaccg tgctgctgat cggccagcag cacggcgacg agccggccgg
     2281 tgccgaggcg ctgatcgtgg tcgcgcagga acttgcaggc ggcgcgctcg cgccggtgct
     2341 ggaccgcatc gacgtcgtga tcctggcgcg cgccaacccc gacggcgcgg ccgccggcac
     2401 gcgcctgacc gccagcggca tcgacgcgaa ccgcgaccac ctgctgctgc gcacgcccga
     2461 ggcgcaggcg gtggcgcaac tggcgcgcga gttcgacccg gcggtcgtgg tcgatgcgca
     2521 cgagtacgcg gcgatcggcc gctacccgca gaagttcggc gcggtgcagc gtttcgacgt
     2581 gctgatccag tacgcgatga cggcgaacct gcccgagttc gtcaccaagg
//
LOCUS       DATMJB010000417         2331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_777983, whole genome shotgun sequence.
ACCESSION   DATMJB010000417 DATMJB010000000
VERSION     DATMJB010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777983"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /locus_tag="VNU48_09005"
     CDS             complement(<1..1557)
                     /locus_tag="VNU48_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HWI11452.1"
                     /translation="MKSNQENQMTAKWRARALTLALSLGACVSALAQNAIQSIDSSQQ
                     SGSEVIRVELSEALTAVPAGFAIQTPPRIAIDLPGVGNALGKSSVEVNQGNLRSVNVA
                     QAGDRTRLVLNLKKPANYRAELQGKVLLVVLDPSAPPEAAVAAAASPAAPANFAPSQN
                     RNPLALKDIDFRRGADGAGRVVVELPNSQVGVDIRQQGQGLVVEFLRSTLPENLRKRL
                     DVTDFGTPVQSITSTQNGDRVRMVVEPRGNWEHSAYQSDNQFVLEVRQRKIDPNKLTQ
                     GPGYAGEKLSLNFQNIEVRALLQVIADFTNFNVVTSDTVTGNVTLRLKDVPWDQALDI
                     ILQAKGLGLRKSGNVILIAPKDELNAKEKVELEAKAQIASLEPLRTQSFQLNYAKAAD
                     IANGLTGQGSSGGAAGNNSRILSTRGTVIFETRTNQLFVSDTPTKLQEIQDMITKIDI
                     PVRQVLIESRIVIADDEFGRSLGVKLGFNDARGIRGGIPGANVGGNTYATIGGNLNAV
                     GAQTLQPGATA"
     gene            complement(1554..2066)
                     /locus_tag="VNU48_09010"
     CDS             complement(1554..2066)
                     /locus_tag="VNU48_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HWI11453.1"
                     /translation="MALGVVAFALTACSGDQQELTQWMEQQRREVKPSVQPLSPPKKF
                     EPQPYAMLGSVEPFSNQKLTVALKQEAKQPNSLLSAELNRRKEPLEAYPLDSMSMVGS
                     VVRAGRPYALLRVDNLLYQVKAGDYLGQNYGKITKISETDISLREIVQDAAGEWIERS
                     SALQLQEKAR"
     gene            complement(2096..>2331)
                     /gene="pilO"
                     /locus_tag="VNU48_09015"
     CDS             complement(2096..>2331)
                     /gene="pilO"
                     /locus_tag="VNU48_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HWI11454.1"
                     /translation="LPIAIRVSGRYHDIGAFAADIANLSRIVTLHNMNITAAPGNTSG
                     ALAMEATARTYRYLDTTEVEAMKSKTAKPGAKK"
BASE COUNT          350 a          769 c          743 g          469 t
ORIGIN      
        1 ggctgtcgca cccggctgca gtgtttgtgc cccaacggca ttgaggttac cgccaatggt
       61 tgcgtaggtg ttgcccccga cattggcgcc agggatgccg ccccgtatgc cacgcgcgtc
      121 gttgaaaccg agcttgaccc ccagcgatcg cccgaactcg tcatcggcaa tgacgatccg
      181 cgattcgatc agcacctgcc gcaccgggat gtcgatcttc gtgatcatgt cctggatttc
      241 ctgcagcttc gtcggcgtgt cgctgacgaa cagctggttc gtgcgtgtct cgaagatgac
      301 ggtgccgcgg gtcgacagaa tccgcgagtt gttgcccgcc gcaccgccgg aggaaccctg
      361 accggtcaac ccgttggcga tgtcggcggc cttcgcgtag ttcagctgga acgactgcgt
      421 gcgcagcggc tcgagcgagg cgatctgcgc cttagcctcg agctcgacct tttccttggc
      481 gttcaactcg tccttcggcg cgatcaggat cacgttgccc gacttgcgca ggcccaggcc
      541 cttggcctgc aggatgatgt ccagcgcctg atcccatggc acgtccttga gccgcagcgt
      601 gacgttgccg gtgacggtgt cgctggtcac gacgttgaag ttcgtgaagt cggcgatgac
      661 ctgcaacagc gcacgcacct cgatgttctg gaagttcagc gacagcttct cgccggcata
      721 gcccggccct tgcgtcagct tgttcgggtc gatcttgcgc tggcgcacct cgagaacgaa
      781 ctggttgtcg ctctgatagg cgctgtgttc ccagttgccg cgcggctcga cgaccatgcg
      841 gacgcggtca ccgttctgcg tgctcgtgat ggattgcacc ggcgtgccga agtcggtcac
      901 gtccagacgc ttgcgaaggt tctccggcaa ggtcgaacgc aggaactcga ccaccagacc
      961 ctgaccctgc tggcggatgt cgacgccgac ctggctgttg ggcagttcga cgacgacgcg
     1021 ccccgcgccg tcggcgccgc gccggaaatc gatgtccttc agcgccagcg ggttgcggtt
     1081 ctgactcggt gcgaaattcg ccggcgctgc aggcgaggcg gcggccgcca cggcggcttc
     1141 cggcggggcc gatgggtcga gcacgaccag cagcaccttg ccctgcagct cggcgcggta
     1201 gttggcgggc ttcttcaggt tcagcactag acgcgtgcgg tcaccggcct gtgcgacgtt
     1261 gaccgagcgc aggttcccct ggttgacctc gacgctggac ttgccgagcg cattgcccac
     1321 gccgggcagg tcgatcgcga tgcgtggagg cgtctggatc gcgaagccgg cgggcaccgc
     1381 ggtcagtgcc tccgacaact cgacgcggat cacttccgag ccggattgct ggctgctgtc
     1441 gatcgactgg atggcgttct gggccagcgc cgaaacgcag gcacccaacg acaacgcgag
     1501 cgtcaaggca cgggcgcgcc acttagctgt catctgattc tcctgattgc tcttcatcgc
     1561 gccttctcct gaagctggag ggcgctgctg cgctcgatcc attccccggc tgcatcctgg
     1621 acgatttccc tgagcgaaat atcggtctcc gaaatcttcg tgatcttccc gtagttctgc
     1681 ccgaggtagt cgcccgcctt gacctggtac agcaggttgt cgacgcgcag cagcgcatac
     1741 ggccgaccgg ctctgacgac actgccgacc atcgacatgc tgtcgagcgg atacgcttcg
     1801 agcggctcct tgcggcggtt gagctccgcc gacagcaagg aattcggctg cttcgcctcc
     1861 tgcttcagcg ccaccgtcag cttctgattg ctgaacggct cgacgctgcc gagcatcgcg
     1921 tacggctgcg gctcgaactt cttcggtggc gacagcggct ggacgctagg cttcacctcg
     1981 cgccgctgct gctccatcca ttgggtcagt tcctgttgat cgccgctgca cgcggtcagc
     2041 gcgaacgcga ccacgcccaa ggccatcagc gatcgcgacg gtgcgggtgt cgccctcatt
     2101 tcttcgcccc tggcttggcc gtcttgctct tcatcgcctc gacctcggtg gtatcgagat
     2161 agcggtacgt ccgagccgtc gcttccatcg ccagcgcgcc cgacgtgttg cccggcgccg
     2221 cggtgatgtt catgttgtgc agcgtgacga tgcgcgacag gttcgcgatg tcggccgcga
     2281 acgcgccgat gtcgtggtag cggcccgaca cgcgaatcgc gatcggcagc t
//
LOCUS       DATMJB010000418         2846 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_168234, whole genome shotgun sequence.
ACCESSION   DATMJB010000418 DATMJB010000000
VERSION     DATMJB010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168234"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VNU48_09020"
     CDS             <1..864
                     /locus_tag="VNU48_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWI11455.1"
                     /translation="DPRSDLFALGVMLYHLATGARPFGAPTSVRGLRQRLYRDPTPPR
                     ALRPDCPPWLQEVILRCLEVKPERRYQSGAQLAFDLRHPGQVTLTERAGRTKRSGKLR
                     EMRRWFAAIGAEPAADASAAAQTARSRILMAAVDVAGADAALLASLRESVQRLLRTEP
                     DTRLACVTVMKTNRIAMDELTDKDGRSKHVNLLVQLKNWARPLAQAVAPASGVPDGRI
                     TFQVLEAPDAASAIVDYARKNRVDQIVMGARSSGGLRRYLGSVSAGVAADADCTVTVV
                     RAPASARGSGP"
     gene            936..1565
                     /gene="kynB"
                     /locus_tag="VNU48_09025"
     CDS             936..1565
                     /gene="kynB"
                     /locus_tag="VNU48_09025"
                     /EC_number="3.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylformamidase"
                     /protein_id="HWI11456.1"
                     /translation="MNNRLWDISPPVGPGSPLFPGDTAYSQQWTAQIGPGRPVNLSAI
                     TMSPHIGAHADAPLHYGPGAPTIGEVDLTAYLGPCRVIHAIGCGPLVRPEHLIHAVDG
                     LPPRVLVRTCAKAPTAWSPEFAAFAPETIAWLASIGVRLVGIDSQSVDPADSKRLDSH
                     QLLLRHDLRVLENLVLDEVPAGDYELIALPLKLITADASPVRAVLRELG"
     gene            complement(1622..>2846)
                     /locus_tag="VNU48_09030"
     CDS             complement(1622..>2846)
                     /locus_tag="VNU48_09030"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HWI11457.1"
                     /translation="GLPPGMEDDPFFKFFRGMPGFQQRGPRGGQVPFRGLGSGFIISS
                     DGLILTNAHVVREAKDVTVKLSDRREFSAKVLGSDPTTDIAVLRVNAKDLPTVRIGDA
                     RNLEVGDPVLAIGAPYGLEQTATQGIVSAKGRSLPGDNTVVPFIQTDAAVNPGNSGGP
                     LFDGTGTVVGINAQIYSQSGGFQGLSFSIPINVAMKVKDQIVATGKASHARLGVTIQD
                     LNQSLAESFGLKRPDGALVANVADGSAAAAAGLKSGDVITEVNGQPVVRSGELSSLIG
                     MAAPGERVKLKVWRDHAAREIDAKLGGAEAKDTELADAGGAVQPGQLGLSMRPLTRDE
                     RREARLEQGLVIENVAGPAAKAGIEPGDVLLAINGRPVQSVEQVKLVLQGKPKSVALL
                     VQRDGDKIFVPVNLG"
BASE COUNT          392 a         1056 c          992 g          406 t
ORIGIN      
        1 gacccgcgca gcgacctgtt cgcgctcggc gtgatgctct accacctcgc caccggcgcg
       61 cgcccgttcg gcgcgccgac cagcgtacgc ggcctgcgcc agcgcctgta ccgcgacccg
      121 acgccgccgc gcgcgctgcg cccggactgc ccgccgtggc tgcaggaagt catcctgcgc
      181 tgcctcgagg tcaagcccga acggcgctac cagagcggtg cgcaactcgc gttcgacctg
      241 cggcatcccg ggcaggtgac gctgaccgaa cgcgccggcc gcaccaagcg cagcggcaag
      301 ctgcgcgaga tgcggcgctg gttcgccgcg atcggcgccg agccggccgc cgacgcgagc
      361 gccgcggcgc agaccgcgcg cagccggatc ctgatggcgg cggtcgacgt cgccggtgcc
      421 gatgccgcgc tgctggcgag cctgcgcgag agcgtgcagc gcctgctgcg caccgagccc
      481 gacacgcggc tggcctgcgt gaccgtgatg aagacgaacc gcatcgcgat ggacgagctg
      541 accgacaagg acggccgcag caagcacgtc aacctgctgg tgcagctgaa gaactgggcc
      601 cggccgctgg cgcaggccgt cgcgccggcc agcggcgtgc ccgacggccg catcaccttc
      661 caggtgctgg aggcgcccga tgcggcgagc gcgatcgtcg actatgcgcg caagaaccgg
      721 gtcgaccaga tcgtgatggg cgcgcgcagc agcggcggcc tgcgccgcta cctgggcagc
      781 gtgtcggccg gtgtggccgc cgacgccgac tgcaccgtga cggtggtgcg cgcgccggcc
      841 tcggcgcgcg gcagcggccc ctgatcggat cgcagcggcg gcgtgcgaaa aaaccgacac
      901 cacgcgcgaa tcggcggtgc taccttcgcg ctgcgatgaa caacagactc tgggacatct
      961 ccccgcccgt cgggcccggc tcgccgctct tccccggcga caccgcctac tcgcagcagt
     1021 ggaccgcgca gatcggcccc ggccgcccgg tgaatctttc cgcgatcacg atgtcgccgc
     1081 acatcggcgc gcatgccgat gcgccgctgc actacggccc cggcgcgccg acgatcggcg
     1141 aggtcgacct gacagcgtat ctcggcccgt gccgcgtgat ccacgcgatc ggatgcggcc
     1201 cgctggtgcg gcccgagcac ctgatccatg cggtcgacgg cctgccgccg cgcgtgctgg
     1261 tgcgcacctg cgcgaaggca ccgaccgcgt ggtcacccga gttcgcggcg ttcgcgccgg
     1321 agacgatcgc gtggctggca tcgatcggcg tgcggctcgt gggcatcgac agccagtcgg
     1381 tcgacccggc cgacagcaag cgcctggaca gccaccagct gctgctgcgc cacgaccttc
     1441 gcgtgctcga gaacctcgtg ctcgacgagg tgccggccgg cgactacgag ctgatcgcgc
     1501 tgccgctgaa gctgatcacc gccgacgcct cgcccgttcg cgcggtcctg cgcgaactcg
     1561 ggtgacggcc gtgctgcgga cctgacgaca acccgccaag cggctggctg gcctctcagg
     1621 ctcagccgag gttgacggga acgaagatct tgtcgccgtc gcgctgcacc agcagcgcca
     1681 cgctcttcgg cttgccctgc agcaccagct tgacctgttc gaccgactgc accgggcggc
     1741 cgttgatcgc gagcagcacg tcgcccggct cgatgccggc cttcgccgcc gggccggcga
     1801 cgttctcgat caccaggccc tgctcgagcc tggcctcgcg gcgctcgtcg cgcgtcagcg
     1861 gccgcatgct caggccgagc tggccaggct gcacggcgcc accggcgtct gccagttcgg
     1921 tgtccttggc ttcggcgccg ccgagcttgg cgtcgatctc gcgcgccgca tggtcacgcc
     1981 agaccttgag cttcacacgc tcgcccggcg ccgccatgcc gatcaggctc gacagttcgc
     2041 ccgagcgcac caccggctgg ccgttgacct cggtgatcac gtcgcctgac ttcaggcccg
     2101 ccgccgccgc ggcgctgccg tcggccacgt tggcgaccag cgcgccgtcg gggcgcttca
     2161 ggccgaagga ctccgcgagc gactggttca ggtcctggat cgtcacgccc agccgcgcgt
     2221 gcgaggcctt tccggtggcg acgatctgat ccttgacctt catcgcgacg ttgatcggga
     2281 tcgagaacga cagcccctga aagccgccgc tttgcgaata gatctgcgcg ttgatgccga
     2341 ccaccgtgcc ggtgccgtcg aacagcgggc cgcccgagtt gccggggttc actgcggcgt
     2401 cggtctggat gaagggcacg accgtgttgt cgcctggcag cgagcggccc ttggcgctga
     2461 cgatgccctg cgtcgcggtc tgctcgagac cgtaaggcgc gccgatggcc agcaccgggt
     2521 cgccgacctc gaggttgcgc gcgtcgccga tgcgcacggt cggcaggtcc ttcgcgttga
     2581 cgcgcagcac cgcgatgtcg gtggtcgggt ccgagcccag caccttggcg ctgaactcgc
     2641 gccggtcgct gagcttgacg gtcacgtcct tggcttcgcg cacgacgtgc gcgttggtca
     2701 ggatcaggcc gtccgagctg atgatgaagc ccgagcccag gccgcggaac ggcacctggc
     2761 cgccgcgcgg gccgcgctgc tggaaccccg gcatgccgcg gaagaacttg aagaacgggt
     2821 cgtcctccat gccgggcggc aggccc
//
LOCUS       DATMJB010000419         3855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_31967, whole genome shotgun sequence.
ACCESSION   DATMJB010000419 DATMJB010000000
VERSION     DATMJB010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31967"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VNU48_09035"
     CDS             complement(<1..702)
                     /locus_tag="VNU48_09035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HWI11458.1"
                     /translation="MPVITHIEDLRVLAEKRVPRMFYDYADSGSYTESTYRANQSDFQ
                     KIKLRQRVAVNMENRTLRTQMVGIDSAMPVAIAPTGLAGMQHADGEILGALAAKKFGI
                     PFTLSTMSICSIEDIAQATRAPFWFQLYWMRDRDFMDRLMDRAKAANVGALMLTLDLQ
                     VLGQRHKDLKNGMTAPPKPTLANILNLMTKPRWCLGMAGTRRHGFGNLIGHAKGVSDT
                     SSLVSWTRERFDPKLD"
     gene            complement(809..2038)
                     /gene="dinB"
                     /locus_tag="VNU48_09040"
     CDS             complement(809..2038)
                     /gene="dinB"
                     /locus_tag="VNU48_09040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWI11459.1"
                     /translation="MPAAPRRLIAHLDMDAFYASVELLRYPDLRGRAVVIGGGRRHQP
                     VLHADGTRTYALLRDYAGRGVVTTSTYEARALGVFSAMGVMKAARLAPDAVLLPVDFD
                     EYRKYSKQFKAAVRALAPVVEDRGVDEIYIDLTDVPGAQRDAGRAIAQRLKDAVLEAT
                     GGLTCSIGVTPNKLLSKLASELDKPNGLTLLGPDDIEARIWPLAAKKVNGIGPKASEK
                     LASFGLHTVGDIAAADPQWLVEHFGKSYGAWLHAASHGRDERPVVTFSEPRSISRETT
                     FERDLHAVRDRATLGAIFTELCEQLAGDLARKGYASRTIGVKLRFDDFRIVTRDLSLP
                     AHTLDARTIRRAAGECLKRVDLTRRLRLLGVRAGALATLAELVAPQATGGDAAPALRE
                     PAASYTLPLFDGVPPPH"
     gene            2151..3347
                     /locus_tag="VNU48_09045"
     CDS             2151..3347
                     /locus_tag="VNU48_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWI11460.1"
                     /translation="MQAVAIANVDTPLVRRAGRELLSLALIDARNHSLRWAAAFEAAP
                     AGVAPLLWELGRLGWFQEYWIARNVQRQRGERCDATRPKLASILPDADVLFEADAAPA
                     SPLPDLQTVRQYLVETLETTLELLDGAARDDESDDALYFYRLALLREDACVERFAVLA
                     QTLGFDTGLVAMPPTRPAREPLLFPATRWMLGSAPGGFVPDRERWAHEVELPEFEIDA
                     QAVTWAQYGEFVGDGGYDDRRHWSAAGWDWVQAQGRRTPRHVDQMRQGVLQRRFGKLM
                     RMPLAQPVVHVSWFEADAWCRWAGRRLPGEAEWEAAAVQGATRGWRWGEVREWTAGTL
                     RPYPGAGAASPGQQPDSTGADAPVARVRRGASFATPGRLRSEKARDAAVPGDDEAFCG
                     FRSCAI"
     gene            3490..>3855
                     /locus_tag="VNU48_09050"
     CDS             3490..>3855
                     /locus_tag="VNU48_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWI11461.1"
                     /translation="MSVPMSLTVNGRAVTADVDPRTLLVQLIRETLQLTGTHVGCDTA
                     QCGACTVHMNGRAVKACSMLALQAQGAQITTIEGLAQNGTMHPMQEAFRECHGLQCGF
                     CTPGMIMAVAGFLKDNPNPS"
BASE COUNT          524 a         1328 c         1420 g          583 t
ORIGIN      
        1 gtcgagcttc gggtcgaacc gctcgcgcgt ccagctcacg agggagctcg tgtcgctgac
       61 acccttcgca tgcccgatca agttgccgaa gccgtggcgc ctggtgccgg ccatgcccag
      121 gcaccagcgc ggcttggtca tcaggttcag gatgttcgcg agcgtcggct tgggcggtgc
      181 cgtcatgccg ttcttgaggt ccttgtggcg ctggccgagc acctgcaggt ccagcgtgag
      241 catcagtgcg ccgacgttgg cggccttggc gcggtccatc agccggtcca tgaagtcgcg
      301 gtcgcgcatc cagtagagct ggaaccagaa cggcgcgcgc gtcgcctgcg cgatgtcctc
      361 gatcgagcag atgctcatcg tgctgagcgt gaacgggatg ccgaacttct tcgcggcgag
      421 cgcgcccagg atctcgccgt cggcgtgctg catgccggcg aggccggtgg gcgcgatcgc
      481 caccggcatc gcgctgtcga tgcccaccat ctgcgtgcgc agcgtgcggt tctccatgtt
      541 gaccgcgacg cgctggcgca gcttgatctt ctggaagtcg ctctggttgg cgcggtaggt
      601 gctctcggtg tacgaacccg aatcggcgta gtcgtagaac atgcgcggca cgcgcttttc
      661 ggccagcacg cgcaggtcct cgatgtgggt gatcacgggc atcgcgacct ctcctgattc
      721 ttggaagttt gccgctcact ctacggcatg cgctgtccag cacacgctgc aacccgagtt
      781 ggcgggctgg gcccgagctc gcgtgcgctc agtgaggtgg cggcacgccg tcgaacagcg
      841 gcagcgtgta gctcgccgcc ggttcgcgca gcgccggtgc cgcgtcgccg cccgtcgcct
      901 gcggtgcgac cagctcggcg agcgtggcca gcgcgccggc acgcacgccg agcaggcgca
      961 ggcgccgcgt caggtcgacg cgcttcaggc actcgccggc ggcgcggcgg atggtgcgcg
     1021 cgtcgagcgt gtgcgccggc aggctgaggt cgcgcgtgac gatcctgaaa tcatcgaagc
     1081 gcagcttgac gccgatcgtg cggctcgcgt aacccttgcg cgcgaggtcg ccggcgagct
     1141 gctcgcacag ctcggtaaag atcgcgccga gcgtggcgcg gtcgcgcacc gcgtgcaggt
     1201 cgcgctcgaa ggtggtctcg cggctgatcg agcggggctc gctgaaggtc acgaccgggc
     1261 gctcgtcgcg cccgtgcgag gcggcgtgca gccacgcgcc gtagctcttg ccgaagtgct
     1321 cgaccagcca ctgcgggtcg gccgcggcga tgtcgccgac ggtgtgcagc ccgaacgacg
     1381 cgagcttctc gctggccttc gggccgatgc cgttgacctt cttcgcggcc agcggccaga
     1441 tgcgcgcctc gatgtcgtcg gggccgagca gcgtcaggcc gttcggcttg tcgagctcgg
     1501 acgcgagctt ggacagcagc ttgttcggcg tcacgccgat cgagcaggtc aggccgccgg
     1561 tggcctcgag caccgcgtcc ttcaggcgtt gcgcgatcgc acggccggcg tcgcgctgcg
     1621 cgccgggcac atcggtcagg tcgatgtaga tctcgtcgac gccgcggtcc tcgacgaccg
     1681 gcgcgagcgc gcgcaccgcg gccttgaact gcttcgagta cttgcggtac tcgtcgaagt
     1741 cgaccggcag cagcaccgcg tcgggcgcga gccgggccgc cttcatcacg cccatcgccg
     1801 agaacacgcc gagcgcgcgc gcctcgtagg tgctggtggt gacgacgccg cggccggcgt
     1861 agtcgcgcag cagcgcgtac gtgcgcgtac cgtcggcgtg cagcaccggc tgatgccgcc
     1921 ggccgccgcc gatcacgacc gcgcgaccgc gcaggtcggg gtagcgcagc agctcgaccg
     1981 acgcgtagaa cgcgtccatg tcgaggtggg cgatcaggcg ccggggggcc gcgggcatgg
     2041 accggattgt ggtgcgaggc tggtattttg tacagtatcg cggcgtcgcc ggcgccgggc
     2101 ccgatgccac aatggaccgc aggcggcatc ccgcgccgca ggagttgctc atgcaggccg
     2161 tcgccatcgc gaacgtggac acgccgctgg tgcgccgcgc cggccgcgaa ctgctgtcgc
     2221 tggcgctgat cgacgcgcgc aaccacagcc tgcgctgggc tgcggcgttc gaggcggcac
     2281 cggccggcgt cgcgccgctg ctctgggagc tcggccggct cggctggttc caggaatact
     2341 ggatcgcgcg caacgtgcag cgccagcgcg gcgagcgctg tgacgcgacg cggcccaagc
     2401 tcgcgtccat cctgcccgat gccgacgtgc tgttcgaggc cgacgcggca ccggcgtcgc
     2461 cgctgcccga tctgcagacc gtgcgccagt acctggtcga gacgctggag acgacgctcg
     2521 agctgctcga tggcgccgcc cgcgatgacg aaagcgacga cgcgctctac ttctatcgcc
     2581 tggccctgct gcgcgaggac gcctgcgtcg agcgcttcgc ggtgctggcg cagacgctgg
     2641 gcttcgacac cgggctggtc gcgatgccgc cgacgcggcc ggcgcgcgag ccgctgctgt
     2701 tcccggcgac gcgctggatg ctcggcagcg cgcccggcgg cttcgtgccc gatcgcgagc
     2761 ggtgggcgca cgaggtcgag ctgccggagt tcgagatcga cgcgcaggcc gtgacctggg
     2821 cgcagtacgg cgagttcgtc ggcgacggcg gctacgacga ccgccgccac tggagcgcgg
     2881 ccggctggga ctgggtgcag gcgcagggcc ggcgcacgcc gcgtcatgtc gaccagatgc
     2941 gccagggtgt gctgcagcgc cgcttcggca agctgatgcg catgccgctc gcgcaaccgg
     3001 tggtgcacgt cagctggttc gaggccgacg cctggtgccg ctgggccggt cgccgcctgc
     3061 ccggcgaggc cgaatgggag gccgcggcgg tgcagggtgc gacgcgcggc tggcgctggg
     3121 gcgaggtgcg cgaatggacg gcgggcacgc tgcggcccta tccgggcgcc ggtgcggcgt
     3181 cgccgggcca gcagccggac tcgaccggcg ccgacgcgcc ggtcgcgcgc gtgcggcgcg
     3241 gcgcatcgtt cgcaacgccc ggccggctgc gcagcgagaa ggcgcgcgac gccgcggtgc
     3301 cgggcgatga cgaagccttt tgcgggttcc gcagctgcgc gatctgagcg ggccgtgacg
     3361 cggtcgaggc acggccggcc ggcataggga aaccccgcgc tgtgcatcgg ccgatcacgc
     3421 tcgaacaatg ggccacgtgt cgcgcccctc atgccgacct cgattccccc gctttcacag
     3481 gagaccaccg tgagcgttcc catgtcattg accgtcaacg gacgggccgt caccgccgac
     3541 gtcgacccgc gcacgctgct cgtgcagctg atccgcgaga ccctgcaact caccggcacg
     3601 cacgtcggct gcgacaccgc gcaatgcggc gcctgcaccg tgcacatgaa cgggcgcgcg
     3661 gtgaaggcgt gctcgatgct cgcgctgcag gcgcagggcg cgcagatcac gacgatcgaa
     3721 ggtctggcgc aaaacggaac catgcatccg atgcaggagg ccttccgcga atgccatggc
     3781 ctgcagtgcg gcttctgcac gccgggcatg atcatggccg tcgcaggttt cctcaaggac
     3841 aatccgaatc cgagc
//
LOCUS       DATMJB010000420         6100 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_684160, whole genome shotgun sequence.
ACCESSION   DATMJB010000420 DATMJB010000000
VERSION     DATMJB010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684160"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1126)
                     /gene="queA"
                     /locus_tag="VNU48_09055"
     CDS             complement(56..1126)
                     /gene="queA"
                     /locus_tag="VNU48_09055"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWI11462.1"
                     /translation="MTSSASRYTLSDFDFALPPELIAQRPAAERSGARLLDGRGAAPV
                     DRVFRDLPALLQPGDLLVFNDTRVIKARLLGAKASGGAVEALVERVLPGHEVLMHLRV
                     SKSPKPGSRVRFAGAFDAEVLGRAGPQDALFHLRFPDDPFTLLERHGHVPLPPYITHA
                     DTADDERRYQTVFAAHPGAVAAPTAALHFDEALLAALVQRGVARANLTLHVGAGTFQP
                     VRVENLAEHTMHSEWFDVPQSTVDAIARTRAAGGRIVAVGTTTLRALESAARGGALQP
                     GSRDTDIFITPGFAFRVVDGLVTNFHLPKSTLMMLVSAFAGFEPMRALYAHAIRERYR
                     FFSYGDAMLLQREPAKGIHAEF"
     gene            1177..3294
                     /gene="recG"
                     /locus_tag="VNU48_09060"
     CDS             1177..3294
                     /gene="recG"
                     /locus_tag="VNU48_09060"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HWI11463.1"
                     /translation="MTAAPATSAGKDKSGPQRALDKLGLRRPIDFALHLPMRYEDETR
                     LVTIASLRDGVTAQVEGVVRDCEVQLRGRRQLVVRVSDGSDDLVLRFLHFYPSQQKQL
                     AAGRRVRVRGEARGGFFGLEMVHPAVKLVAEGAPLPSALTPVYPTSAQLPQAYLRKAM
                     DAALSRVDLSELLPPEAVPPGMMSLRDALNFLHHPAPNVGLATLEDRSHPAWQRLKFE
                     ELLAQQLSQLQAKRERERRRAPRFAVRAGALQEKLLAALPFELTGAQRRVVEQIANDL
                     QRGNRVEADGMPSTVPMHRLLQGDVGSGKTVVAALAAAIAIADGWQCALMAPTEILAE
                     QHLRKLVGWLAPLGITVAWLTGSRKGKARTRMLEQVASGEAALVVGTHAVIQEQVHFA
                     KLGLAIVDEQHRFGVQQRLALREKLRSPSQGADGVGAGAIEPHTLMMSATPIPRTLAM
                     TLFADLDVSTIDELPPGRTPVVTKLFADSKRAAVVERIRDAVAQGQQVYWVCPLIEES
                     ATLDLRNATETHAQLSAALPGHRVGLLHGRMPAAEKAAAMALFTTGAMAVLVSTTVIE
                     VGVDVPNASLMVIEHAERFGLSQLHQLRGRVGRGSVASVCVLLYTAPLSDTGKARLKA
                     MLETGDGFEIARRDLEIRGPGEFLGARQSGAPLLRFADLAQDEALVHAARASATLLLD
                     RYPQAALDHVTRWLGGRSDYLKA"
     gene            3353..4291
                     /locus_tag="VNU48_09065"
     CDS             3353..4291
                     /locus_tag="VNU48_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWI11464.1"
                     /translation="MTLTELRYIVAVAREKHFGRAAEACFVSQPTLSVSIKKLEEELD
                     VKIFERGANEVTVTPLGNDIVRQAQTVLEEAAAIKEIAKRGKDPVSGPLGLGVIYTIG
                     PYLLPDLVRQAIEHVPQMPLMLQENFTAKLLEMLRTGELDCAIMAEPFPDTGLAVAPL
                     YDEPFMAAVPKTHPLAKRKSVSTDELKSETMLLLGTGHCFRDHVLEVCPEYARFGSSD
                     EGSAKGVGIRQSFEGSSLETIKYMVASGMGVTVVPQLSVPKDAQPHIKYLPFTKPAPT
                     RRVVLVWRRTFTRYEAIAALRNAIYACELPGVKRLS"
     gene            4386..4883
                     /locus_tag="VNU48_09070"
     CDS             4386..4883
                     /locus_tag="VNU48_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HWI11465.1"
                     /translation="MARNKDARVGIHIGISDKDRSTISAGLSRLLADTYTLYLTTHNF
                     HWNVTGPMFSSLHAMFMVQYTELWNAVDPIAERIRSLGHTAPGSYAQFAGLATVPDAP
                     ATPPKALDMVRLLVAGHETVARTARGIFPLADKASDEPTADLLTQRLTVHEQSAWMLR
                     SLLEE"
     gene            4927..5550
                     /locus_tag="VNU48_09075"
     CDS             4927..5550
                     /locus_tag="VNU48_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWI11466.1"
                     /translation="MRIAILTFDGFNELDSFIVFGLLNRLGAQGWKAELASPSSHVTS
                     MNGVTVQAQQPLEFANEADAVIFGSGIYSRSIADPGSERGAWLDRLELDPVRQMIGAQ
                     CSGALLMARLGLLADVPACTDLTTKPWLIETGVRVDDASFRARGPIATAGGCLSSQYL
                     AAWLIASRLGFAAAAQSLAMSAPVGEREAYVARLLDEVRPFINNPKS"
     gene            5698..>6100
                     /locus_tag="VNU48_09080"
     CDS             5698..>6100
                     /locus_tag="VNU48_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWI11467.1"
                     /translation="MLTLHYHPGNASLAPHLLLEEMGVPFALVLVDRAHDAHRSTAYL
                     KLNPNGLIPVLTDGAVVLYEAAAICLHLVDTHPEAGLAPALGTPERAHFYKRLVWLTN
                     TLQAALIVYFYPERWADDAAAIAQVKAHAEAK"
BASE COUNT          944 a         2143 c         2159 g          854 t
ORIGIN      
        1 gccgtggttc agggtcaggc ggccgcggcg cgcgtggcct tcggtcttca gcaaatcaaa
       61 actcagcatg aattcctttc gcgggctcgc gctgcagcag catcgcgtcg ccgtagctga
      121 agaaccgata gcgctcgcgg atcgcgtgcg cgtacagcgc acgcatgggc tcgaagccgg
      181 cgaacgcgct gaccagcatc atcagcgtgc tcttcggcag atgaaagttg gtgacgagcc
      241 catcgacgac gcggaacgcg aagcccggcg tgatgaagat gtcggtgtcg cggctgccgg
      301 gctgcagcgc gccgccacgc gccgccgatt cgagcgcgcg cagggtcgtc gtgccgaccg
      361 cgacgatgcg cccaccggcg gcgcgggtgc gcgcgatggc gtcgaccgtg ctttgcggca
      421 cgtcgaacca ttcgctgtgc atcgtgtgct cggccaggtt ctcgacgcgc accggctgga
      481 acgtgccggc accgacatgc agcgtgaggt tcgcccgcgc gacgccgcgc tgcaccagcg
      541 cggcgagcag cgcctcgtcg aagtgcagcg ccgcggtcgg cgcggccacc gcgccgggat
      601 gcgcggcgaa tacggtctgg tagcggcgct cgtcgtccgc cgtgtcggcg tgcgtgatgt
      661 agggcggcag cggcacgtgg ccgtggcgtt cgagcagcgt gaacggatcg tcggggaagc
      721 gcaggtggaa gagcgcgtcc tgcgggccgg cacggcccag cacctcggcg tcgaatgcgc
      781 cagcgaagcg cacgcggctg cccggcttgg gcgacttgct gacgcgcagg tgcatcaaca
      841 cctcgtgacc cggcagcacg cgctcgacca gcgcctcgac cgcgccgccg ctggccttgg
      901 cgccgagcag ccgcgccttg atcacgcgcg tgtcgttgaa caccagcagg tcgcccggct
      961 gcagcagcgc cggcaggtcg cggaacacgc ggtcgaccgg cgcggcgccg cggccgtcga
     1021 gcaggcgcgc gccgctgcgc tcggccgcgg gccgctgggc gatcagttcg gggggcagag
     1081 cgaagtcgaa atcgctcagc gtgtagcgag aggcgctgga ggtcatcggt tgagggctgg
     1141 accggcccgt gccaacaaga gaggcagaat tctcccatga ccgcagcacc cgcgacctcg
     1201 gccggtaagg acaagtccgg cccgcagcgg gcgctcgaca agctcggcct gcgccgcccg
     1261 atcgacttcg cgctgcacct gccgatgcgc tacgaggacg agacccgcct cgtgacgatc
     1321 gcgagcctgc gcgatggcgt gacggcgcag gtcgaaggcg tggtgcgcga ctgcgaggtg
     1381 cagctgcgcg ggcgccgcca gctcgtcgtg cgcgtctcgg acggcagcga cgacctggtg
     1441 ctgcgcttcc tgcacttcta cccgtcgcag cagaagcagc tcgcggccgg tcggcgcgtg
     1501 cgggtgcgcg gcgaggcgcg tggcggcttc ttcgggctgg agatggtgca cccggcggtc
     1561 aagttggtgg ccgagggcgc accgctgccg agcgcgctga cgccggtcta cccgaccagc
     1621 gcgcagctgc cgcaggccta tctgcgcaag gcgatggatg cggccctgag ccgggtcgac
     1681 ctgtcggaac tgctgccgcc cgaggccgtg ccgccgggca tgatgagcct gcgcgacgcg
     1741 ctgaactttc ttcaccaccc agcgccgaac gtcggcctcg ccacgctcga ggaccgcagc
     1801 cacccggcct ggcagcgcct gaagttcgag gagctgctgg cgcagcagct ctcgcagttg
     1861 caggccaagc gcgaacgcga gcgccggcgc gcgccgcgct tcgcggtgcg cgccggcgca
     1921 ctgcaggaga agctgctcgc ggcgctgccg ttcgagctga ccggcgcgca gcgccgcgtc
     1981 gtcgaacaga tcgcgaacga cctgcaacgc gggaatcgag ttgaggccga cggcatgccg
     2041 tcgaccgtgc cgatgcaccg tctgctgcaa ggcgatgtcg gctccggcaa gaccgtggtg
     2101 gcggcgctgg ccgccgcgat cgcgatcgcc gacggctggc agtgcgcgtt gatggcgccg
     2161 accgagatcc tcgccgagca gcacctgcgc aagctggtcg gctggctcgc gccgctgggc
     2221 atcacggtgg cgtggctgac cggcagccgc aagggcaagg cgcgcaccag gatgctggaa
     2281 caggtggcgt cgggcgaggc ggcgctggtg gtcggcacgc acgcggtgat ccaggagcag
     2341 gtgcatttcg cgaagctcgg gctcgcgatc gtcgacgagc agcaccgctt cggcgtgcag
     2401 cagcgcctgg ccctgcgaga gaagctgcgc tcgccctcgc aaggcgccga cggggtcggc
     2461 gccggggcga tcgagccgca cacgctgatg atgagtgcca cgccgatccc gcgcacgctg
     2521 gcgatgacgc tgttcgccga cctcgacgtg agcacgatcg acgaactgcc gcccggtcgc
     2581 acgccggtcg tgaccaagct gttcgccgac tcgaagcgcg ccgcggtggt cgagcgcatc
     2641 cgcgatgccg tcgcgcaggg ccagcaggtc tactgggtct gcccgctgat cgaagagagc
     2701 gcgacgctgg acctgcgcaa cgcgaccgag acgcacgcgc aactcagcgc ggccctgccc
     2761 ggccacaggg tcggcctgtt gcacgggcgc atgccggcgg ccgagaaggc ggcggcgatg
     2821 gcgctcttca ccaccggcgc gatggcggtg ctggtgtcga ccacggtgat cgaggtcggc
     2881 gtcgatgtgc cgaacgcgtc gctgatggtg atcgagcacg ccgagcgctt cggcctgtcg
     2941 cagctgcacc agctgcgcgg gcgcgtcggc cgcggcagcg tggccagcgt ctgcgtgctg
     3001 ctgtacaccg cgccgctgtc ggacaccggc aaggcgcgcc tgaaggcgat gctcgagacc
     3061 ggcgacggct tcgagatcgc gcgccgcgac ctcgagatcc gcgggcccgg cgagtttctc
     3121 ggcgcgcgcc agtcgggcgc gccgctgctg cgcttcgccg acctcgcgca ggacgaggcg
     3181 ctggtgcatg ccgcgcgcgc gtcggcaacg ctgctgctgg accgataccc gcaggccgcg
     3241 ctggaccacg tcacgcgctg gctgggcggc cgctcggact acctgaaggc ctgatccgcc
     3301 gccgcggggc cggcgcttgg cgcacccctg cccttgcggc cagaatcccg ccatgacgct
     3361 caccgaactc cgctacatcg tcgccgtcgc ccgcgagaaa cacttcgggc gcgccgccga
     3421 ggcctgcttc gtctcgcagc ccacgctgag cgtgtcgatc aagaagctcg aggaagagct
     3481 cgacgtgaag atcttcgagc gcggcgcgaa cgaggtcacc gtcacgccgc tgggcaacga
     3541 catcgtgcgc caggcacaga cggtgctgga ggaggccgcg gcgatcaagg agatcgccaa
     3601 acgcggcaag gacccggtct cgggcccgct cggcctgggc gtgatctaca cgatcgggcc
     3661 gtacctgttg cccgacctcg tgaggcaggc gatcgagcac gtgccgcaga tgccgctgat
     3721 gctgcaggag aacttcaccg ccaagctgct cgagatgctg cgtaccggcg agctcgactg
     3781 cgcgatcatg gccgagccct tccccgacac gggcctcgcg gtcgcgccgc tgtacgacga
     3841 gccgttcatg gccgcggtgc cgaagacgca cccgctggcc aagcgcaaga gcgtgagcac
     3901 cgacgagctg aagagcgaga cgatgctgct gctcggcacc ggccactgct ttcgcgatca
     3961 cgtgctcgaa gtctgccccg aatacgcgcg cttcggcagc agcgacgaag gcagtgcgaa
     4021 gggcgtgggc atccgccaga gcttcgaggg ctcgtcgctc gagacgatca agtacatggt
     4081 cgcctcgggc atgggcgtca cggtggtgcc gcagctcagc gtgcccaagg atgcgcagcc
     4141 gcacatcaag tacctcccgt tcaccaagcc ggcgccgacg cggcgcgtcg tgctggtgtg
     4201 gcggcgcacg ttcacgcgct acgaggcgat cgcggcgctg cgcaacgcga tctacgcgtg
     4261 cgagctgccg ggcgtgaagc ggctgtcctg agccgccgag caagcgtgct ggcacactgg
     4321 cgccgcgcgg gcagcggctg cgctacagtg gtcccatcgg cgaacccttt gcggagcgag
     4381 agaacatggc caggaacaag gacgcgcgcg tcggcatcca catcggcatc agcgacaagg
     4441 accgcagcac gatctcggcc ggcctgtcgc gcctgctggc cgacacctac acgctgtacc
     4501 tgacgaccca caactttcac tggaacgtga ccgggccgat gttcagctcg ctgcacgcga
     4561 tgttcatggt gcagtacacc gagctgtgga acgcggtcga cccgatcgcc gaacgcatcc
     4621 gctcgctcgg ccacaccgca ccgggctcgt atgcgcagtt cgccggcctg gcaacggtgc
     4681 cggacgcgcc ggccacgcca ccgaaggcac tcgacatggt gcgcctgctg gtggccggcc
     4741 acgagaccgt ggcgcgcacc gcgcgcggca tcttcccgct ggccgacaag gcgagcgacg
     4801 agcccaccgc cgacctgctg acgcaacgcc tgacggtgca tgagcaatcg gcctggatgc
     4861 tgcgcagcct gctcgaagag taggccttcc ttccacccgc ctgaacccac tcgcgagggc
     4921 ccaccgatgc gcatcgccat cctcaccttc gacggcttca acgagctcga ctcgttcatc
     4981 gtcttcggcc tgctgaaccg cctcggcgcc cagggctgga aggccgagct ggcgagcccg
     5041 agttcgcacg tcacctcgat gaacggcgtg acggtgcagg cgcaacagcc actcgagttc
     5101 gccaacgagg cggacgcggt gatcttcggc agcggcatct actcgcgctc gatcgccgac
     5161 ccggggtccg agcgaggcgc ctggctcgat cggctggaac tcgatccggt gcgccagatg
     5221 atcggcgcgc agtgttcggg cgcgctgctg atggcgcgcc tgggcctgct ggcggacgtg
     5281 ccggcctgca ccgacctgac caccaagccg tggctgatcg aaaccggcgt gcgcgtcgac
     5341 gacgcctcgt tccgcgcccg cgggccgatc gcgacggccg gtggctgctt gtcctcgcag
     5401 tacctcgccg cgtggctgat cgcgagccgg ctcggcttcg cggcggcggc gcaatcgctc
     5461 gccatgtcgg caccggtggg cgagcgcgag gcctacgtcg cgcgcctgct cgacgaggtg
     5521 cggccgttca tcaacaaccc gaagtcctga cccaggccta caccctcacc ccggccctct
     5581 cccgcaagcg ggcgagggag caaaaacggc tccctctccc atgtacccgg gggagagggt
     5641 tggcgtgagg gggcgatgtg caccgaagca ggacctccct ggatccgcgc gaacagcatg
     5701 ctcaccctgc actaccaccc cggcaacgcc agcctggcac cgcacctgtt gctggaggaa
     5761 atgggcgttc cgttcgcgct ggtcctcgtc gatcgcgcgc acgacgcgca caggtcgacc
     5821 gcctacctga agctcaatcc gaacggcctg atcccggtgc tgaccgatgg cgcggtggtg
     5881 ctctacgagg ccgccgcgat ctgcctgcac ctggtcgaca cacatcccga ggctggtctg
     5941 gcgcccgcgc tcggcacgcc cgaacgggcc cacttctaca agcggctggt gtggctcacc
     6001 aacaccctgc aggccgcgct gatcgtctac ttctatccgg aacgctgggc cgacgacgca
     6061 gccgccatcg cgcaggtgaa ggcgcatgcc gaggccaagg
//
LOCUS       DATMJB010000421         2980 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_844524, whole genome shotgun sequence.
ACCESSION   DATMJB010000421 DATMJB010000000
VERSION     DATMJB010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844524"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /gene="purF"
                     /locus_tag="VNU48_09085"
     CDS             <1..1210
                     /gene="purF"
                     /locus_tag="VNU48_09085"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWI11468.1"
                     /translation="LTNAHVLKTELVDIDRRHINTTSDTEVLINIVAHELAVAAHEKT
                     LTTDEIFSAVRAVHKRLKGSYAVVALIAGHGLLAFRDPFGIRPLCLGEAVTPEGRAVM
                     VASESVALEGTGHKLLRDVAPGEAVFVDLEGTVHARQCAEHPTLNPCMFEFVYLARPD
                     SVMDGVSVYQARLNMGETLAQRLISTMPPSDIDVVIPIPESSRPSAMQLAQKIGKPYR
                     EGFVKNRYVGRTFIMPGQGERKKSVRQKLNAIGVEFKGRNVLLVDDSIVRGNTSKEIV
                     QMARDAGARKVYMASAAPPVRYPNVYGIDMPTSEELIAHNRSIEEIRAFIGADALIYQ
                     DVNAMKRVVGALNPKLNGFEASCFDGVYITGDVSAADFTAMAEQRKAQGDDEESGDRS
                     RLALQSGEVV"
     gene            1207..2460
                     /locus_tag="VNU48_09090"
     CDS             1207..2460
                     /locus_tag="VNU48_09090"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HWI11469.1"
                     /translation="MTARDDESDESRRIARAVLPEGLRRETLAVRTALAPSQHGENSE
                     ALFLTTAYVQPNTETMARRFANSEDFTYSRTSNPTVRSFEARLAAMEGAEAAIATSSG
                     MSAILLLGMGLLKAGDHVICSRSVFGSTINLFAKEFAKFGVRTSFVSQTDLAEWRAAL
                     EPATRLLFAETPTNPLTDVCDIRALADLAHEAGALLAVDNSFCSPALQRPVEHGADIV
                     MHSGTKYLDGQGRVMAGALAGSARLMKEVFGPVMRTAGMVLAPFNAWVVLKGMETLDV
                     RMRAQSANTLQVAQWLEQQPEVARVYYPGLASHPQHALAMRQQSELGGALLSFDLRGA
                     SIEAARANAFHVIDSTRLLSIATNLGDTKSIITHPATTSHGRLSEDQRRAAGITQGLV
                     RLAVGLEHPLDVIDDLQRGLSTLNA"
     gene            2457..>2980
                     /locus_tag="VNU48_09095"
     CDS             2457..>2980
                     /locus_tag="VNU48_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HWI11470.1"
                     /translation="MTISKVRTRIAPSPTGFLHLGTARTALYSWAYARHFGGEFVLRI
                     EDTDVARSTQDSVDQILAAMHWLGLDYAEGPVYQMQRLERYRAVVEQMLAAGTAYRCY
                     CTPDELTAMREAQKARGEKTHYDGRWRPAPGKLLPPVPPGVAPVVRFRNPPDGDVSWD
                     DLVKGRITINNREI"
BASE COUNT          518 a         1048 c          985 g          429 t
ORIGIN      
        1 cctgaccaac gcgcatgtgc tgaagaccga actggtcgac atcgaccgcc gccacatcaa
       61 caccaccagc gacaccgagg tgctgatcaa catcgtcgcc catgagttgg ccgtggccgc
      121 gcacgagaag acgctgacca ccgacgagat cttcagcgcc gtgcgggcgg tgcacaagcg
      181 gctgaagggc tcgtacgccg tcgtcgcgct gatcgccggc catggcctgc tggccttccg
      241 cgatccgttc ggcatccgtc cgctgtgcct cggtgaggcg gtgacgcccg aaggccgcgc
      301 ggtgatggtg gcgagcgaat cggtcgcgct cgaaggcact ggccacaagc tgctgcgcga
      361 cgtggcgccc ggcgaggcgg tcttcgtcga cctcgaaggc accgtgcatg cgcgccagtg
      421 cgccgagcat ccgacgctga acccctgcat gttcgagttc gtctacctgg cgcggcccga
      481 ttcggtgatg gacggcgtgt cggtctacca ggcgcggctc aacatgggcg agacgctggc
      541 gcagcgcctg atctcgacca tgccgccgag cgacatcgac gtcgtcatcc cgatccccga
      601 atcgagccgg ccatcggcga tgcagctcgc gcagaagatc ggcaagccgt accgcgaagg
      661 cttcgtgaag aaccgctacg tcggccgcac cttcatcatg ccggggcagg gcgaacgcaa
      721 gaaatcggtg cggcaaaagc tcaacgcgat cggcgtcgag ttcaagggcc gcaacgtgct
      781 gctggtcgac gactcgatcg tgcgcggcaa cacctcgaaa gagatcgtgc agatggcgcg
      841 cgacgccggc gcgcgcaagg tctacatggc ctcggcggca ccgccggtgc gctacccgaa
      901 cgtctacggc atcgacatgc ccacgagcga agagctgatc gcgcacaacc gcagcatcga
      961 ggagattcgc gcgttcatcg gcgccgacgc gctgatctac caggacgtga atgcgatgaa
     1021 gcgcgtcgtc ggcgcgttga atccgaagct caacggcttc gaggcctcgt gcttcgacgg
     1081 tgtctacatc accggcgacg tgagcgctgc cgacttcacg gcgatggccg agcagcgcaa
     1141 ggcgcagggc gacgacgaag agagcggcga ccgcagccgc ctcgcactgc agagcggcga
     1201 ggtggtatga cggcgcgaga cgacgagagc gacgaaagcc gccgcatcgc ccgcgcggtg
     1261 ttgcccgagg gcctgcgccg cgagaccctc gcggtgcgca cggccctcgc gccgagccag
     1321 cacggcgaga actccgaggc gctgttcctg accaccgcct acgtgcagcc gaataccgag
     1381 acgatggcgc ggcgctttgc caattccgag gacttcacct actcgcgcac cagcaacccg
     1441 accgtgcgca gcttcgaggc gcggctggcg gcgatggagg gcgccgaggc cgcgatcgcg
     1501 acgagcagcg gcatgagcgc catcctgctg ctcggcatgg gcctgttgaa ggccggtgac
     1561 cacgtgatct gttcgcggtc cgtgttcggc tcgacgatca atctgttcgc caaggagttc
     1621 gccaagttcg gcgtgcgcac cagcttcgtc tcgcagaccg atctggccga atggcgcgcc
     1681 gcgctcgagc cggcgacgcg cctgctgttt gccgagacgc cgaccaatcc gctcaccgac
     1741 gtctgcgaca tccgggcgct ggcggatctg gcccatgagg ccggcgcgct gctcgcggtg
     1801 gacaacagct tctgctcgcc cgcgctgcaa cgccccgtgg agcacggcgc cgacatcgtg
     1861 atgcactcgg gcacgaaata cctcgacggc cagggccgcg tgatggccgg cgcgctcgcc
     1921 ggctcggctc ggctgatgaa ggaggtgttc ggcccggtca tgcgcacggc cggcatggtc
     1981 ctcgcgccct tcaatgcctg ggtggtcctc aaggggatgg agacgctcga cgtgcgcatg
     2041 cgtgcgcaaa gcgccaacac cctgcaggtg gcgcaatggc tggaacagca acccgaggtc
     2101 gcacgcgtct actatcccgg cctcgcctcg cacccgcagc acgcgctggc catgcgccag
     2161 cagtcggaac tgggcggtgc gctgctgtcg ttcgacctgc gcggcgcgtc gatcgaagcc
     2221 gcgcgcgcca acgcgtttca cgtgatcgac agcacccgcc tgctctcgat cgcgacgaac
     2281 ctcggcgaca ccaagtccat catcacgcac ccggccacca cctcgcacgg gcgcctcagc
     2341 gaagatcagc gccgcgctgc cggcatcacg cagggtctgg tgcgtctggc ggtcggcctc
     2401 gaacacccgc tggacgtcat cgacgacctg cagcgcggcc tcagtacctt gaacgcatga
     2461 ccatcagcaa ggtaagaacg cgcatcgcgc catcgcccac cggcttcctg cacctcggca
     2521 cggcccgcac cgcgctctat tcgtgggcct atgcgcgcca cttcggcggc gagttcgtgc
     2581 tgcgcatcga agacaccgac gtcgcgcgtt cgacgcagga ctcggtcgac cagatcctcg
     2641 cggcgatgca ctggctcggc ctggactacg ccgaaggccc ggtctaccag atgcagcgcc
     2701 tggagcgcta ccgcgccgtg gtcgagcaga tgctggcggc cggcacggcc taccgctgct
     2761 actgcacacc cgacgaactg accgcgatgc gcgaggccca gaaagcgcgc ggcgagaaga
     2821 cccactacga cggccgctgg cgccccgcgc ccggcaagct gttgccgccg gtgccacccg
     2881 gcgtcgcacc ggtcgtgcgc tttcgcaacc cgcccgacgg cgacgtgagc tgggacgacc
     2941 tggtcaaggg ccgcatcacg atcaacaacc gcgagatcga
//
LOCUS       DATMJB010000422         6557 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_229234, whole genome shotgun sequence.
ACCESSION   DATMJB010000422 DATMJB010000000
VERSION     DATMJB010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229234"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VNU48_09100"
     CDS             complement(<1..227)
                     /locus_tag="VNU48_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11471.1"
                     /translation="MALLLHAAALGGLDWAWPTTEPAAQPVAAMQVRVVDPAPEPRPA
                     EAPMNPSPPVKMAEPTAAAPPPRVRTRLVAAR"
     gene            355..1338
                     /locus_tag="VNU48_09105"
     CDS             355..1338
                     /locus_tag="VNU48_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWI11472.1"
                     /translation="MKLATYKDGSRDGQLVVVSRDLTLAHYASGVASRLQQVLDDWNF
                     LSPQLEELSQMLNHGKARHAFAFDPRLCMAPLPRAYQWADGSAFINHVELVRKARGAE
                     VPESFYTDPLMYQGGSDDFLGARDDIVCASVAHGIDFEAEVAVITGDVAMGTAPDAAI
                     DAIRLLMLANDVSLRHLIPAELAKGFGFFQSKPATAFGPVAVTPDELGAAWRHGRVHL
                     NLESVWNGKRVGLCDAGPEMSFHFGELIAHIAKTRNARAGTIVGSGTVSNKDWSRGYS
                     CIAEKRAIETIESGAPKTEFMQAGDTIRLEMKGADGMSVFGAIEQRVVAPA"
     gene            1410..1901
                     /locus_tag="VNU48_09110"
     CDS             1410..1901
                     /locus_tag="VNU48_09110"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HWI11473.1"
                     /translation="MDHDLSHRFKLITVWLLLGAAVFVAIQWWQHRAQQTLFQTRGST
                     VEIRRGDDGHYHWPGRVNGRAVDFLIDTGATGTAIPASLARELELVSLGSISSNTAGG
                     VVSAQVMRADIELQGGVSVRRLQIVALPRLGENPLLGMDVLGRLHWQQRDAVLRIELG
                     NGR"
     gene            1928..2905
                     /locus_tag="VNU48_09115"
     CDS             1928..2905
                     /locus_tag="VNU48_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HWI11474.1"
                     /translation="MTRLRRTALTSLLALLLVGVARAQAAPECPPSAQVPTPEQLQAG
                     ARQARDHGFLWRISKGGRSSWLYGTLHVAKLEWTFPGPAVNAALRASDTIALELDALD
                     PEVQRRMAQALAAQTHAALPPALQQRLDRAAQAECVPAQALAALAPEMQIATLTTLLG
                     RRDGLDASYGIDVVLAGWGHAGKLEVVSLETPELQLRTLQSASPAETVEAVASALDEL
                     EGGRTLPLLLRMARVWADGDWAALTGYASWCECLKTAADRAEMARMLDERNPALADGI
                     AALHARGQRVFAAVGSLHMIGPTGLPALLAQRGYRVERITYNQTSETPP"
     gene            2902..3570
                     /locus_tag="VNU48_09120"
     CDS             2902..3570
                     /locus_tag="VNU48_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhaM family polyhydroxyalkanoate granule
                     multifunctional regulatory protein"
                     /protein_id="HWI11475.1"
                     /translation="MTDPSAFSKLVPGFDFLQGLVKNAGAALPGIGSWVAPTIDPAEL
                     EKRIEELRTVQFWLEQNARMLGATIQALEVQRMTLSTLKTMNVQMEDLRDSLQIRMPT
                     MPPPAAPEPAPAAKATPAPDAEPAPPAPPAGAPAGPEARAGVVDPLQWWGALTKQFTE
                     LATTALKDTASDATRKLAGAAMKRSLDPAGAAPAAPARSAAAKKKKRPAAKKAPRAKA
                     APHD"
     gene            3646..4932
                     /locus_tag="VNU48_09125"
     CDS             3646..4932
                     /locus_tag="VNU48_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HWI11476.1"
                     /translation="MNRFLHGHATHADWRVALALAAAQIEAQRHTPGHAATPTLGWVY
                     LTDALAPHAEALLGELRQRWPGVEWVGATGVGIAAAGVEYFDAPALALMLTDLAPARF
                     RVFSGARPLTGFAAHTANVHADPSAADLAELIHDMSARTATGYLFGGLASAPSRPLHI
                     AGNALAGGLSGVAFDRGVALVSRVTQGCQPVGPQRTVTAAERNVVIALDGEPALDCLL
                     RDIDLSGVDLRSALPRLREVLVGLSDAGDAAEALAPREDASTRSAFDAIARHRSTARA
                     FGADTRVRHLVGLDPARRAIAIADSAEPGVRLAFCARHVEAARRDLMRICAEVREEVE
                     TEELPQRSALALKGVPGADAGSATASGIAGAIYVSCSGRGGAHFGAPSAELGIVRHAL
                     GDVPLVGFFAAGEIARRHLYGYTGVLTVFRRGGDAA"
     gene            4929..6494
                     /locus_tag="VNU48_09130"
     CDS             4929..6494
                     /locus_tag="VNU48_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWI11477.1"
                     /translation="MSPPGCPKGEYRSAQHEGTPVNAALPDALTIEVDERARRQTEVV
                     AALAAVLPAHALLWHREDTVPYECDGLTAYREQPLVVALPETEAEVAAVLRECHRLNV
                     PVVARGAGTGLSGGALPHRLGVTLSLAKFNRILKIDPVARTALVQCGVRNLAISEAAA
                     PYGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGLTLHNVLRVRGFTVDGSAVEFGS
                     EALDCAGLDLMTILVGSEGMLAVTLEVSVKLVPRPQLARCIMASFDDIRKAGDAVANV
                     IAAGIIPAGLEMMDKPMTAAVEDYVHAGYDLAAKAIMLCESDGTPEEVAEEIARMEAV
                     LGTSGATRIEVSDSEAQRLKFWSGRKNAFPASGRISPDYMCMDSTIPRHRLADILLAI
                     QAMETKYGLRCANVFHAGDGNLHPLIMFDANDPDQLHRCELFGADILESSVAMGGTVS
                     GEHGVGIEKLSAMCVQFAPAEREQMLALKRAFDPKELLNPGKAIPSLARCAEYGKMHV
                     RRGLLPFADIPRF"
BASE COUNT          971 a         2381 c         2341 g          864 t
ORIGIN      
        1 cgcgccgcga cgagcctggt gcgcacgcgc ggcggcggcg ccgctgccgt cggttcggcc
       61 atcttgaccg gcggtgaagg gttcatcggc gcttccgcgg gccgcggctc gggcgctggg
      121 tcgaccacac gcacctgcat cgccgcgacc ggctgcgcgg ccggctcggt cgtcggccag
      181 gcccaatcga ggccgccgag cgcggccgcg tgcaacagca gggccacggc gctcagcatg
      241 cccagccgca gccggcgccg gggtgcgccg tcgcgcggcg cagaagaatc gggatcaaag
      301 tcatacgtcg acaatagcgt tccttcgaag accccggagc cgctcggaca tcccatgaag
      361 cttgcgacct acaaggacgg ctcccgcgac gggcagctcg tcgtcgtttc gcgcgacctg
      421 acactcgccc actatgccag cggcgtcgcg agccggctgc agcaggtgct ggacgactgg
      481 aacttcctgt cgccgcagct cgaggagctg tcgcagatgc tgaaccacgg caaggcgcgc
      541 cacgcgttcg cgttcgatcc gcgcctgtgc atggcgccgc tgccgcgcgc ctaccagtgg
      601 gccgatggct cggccttcat caaccatgtc gagctggtgc gcaaggcgcg cggcgccgag
      661 gtgcccgaga gtttctacac cgacccgttg atgtaccagg gcggcagcga cgacttcctc
      721 ggcgcgcgcg acgatatcgt ctgcgcgagc gtggcgcacg gcatcgactt cgaggccgag
      781 gtcgcggtga tcaccggcga cgtcgcgatg ggcaccgcgc ccgatgccgc gatcgacgcg
      841 atccggctgc tgatgctcgc caacgacgtg agcctgcgcc acctgatccc ggccgaactc
      901 gcgaagggct tcggcttctt ccagagcaag ccggcgaccg cgttcggccc ggtcgcggtg
      961 acgccggacg aactcggcgc cgcgtggcgg catggccgcg tccacctgaa cctggagagc
     1021 gtctggaacg gcaaacgcgt cggcctgtgc gacgccgggc cggagatgag cttccacttc
     1081 ggcgagctca tcgcccacat cgccaagaca cgcaacgcgc gcgccggcac catcgtcggc
     1141 agcggcaccg tcagcaacaa ggactggtcg cgcggctact cgtgcatcgc cgagaagcgc
     1201 gcgatcgaga cgatcgagag cggcgcgccg aagaccgaat tcatgcaggc cggcgacacg
     1261 atccgcctcg agatgaaggg cgcggacggg atgagcgtgt tcggggcgat cgagcagcgc
     1321 gtggtggcgc cggcctgagc gggcatcgcg cacacccaac gcgagccgcc gccaaggcac
     1381 ggcacgaaac ccgcagaatc cgtcatcgca tggaccacga cctgtcgcac cgcttcaagc
     1441 tgatcaccgt ctggctgctg ctgggcgccg cggtctttgt cgcgatccag tggtggcagc
     1501 atcgcgcgca gcagaccctg ttccagacgc gcggcagcac ggtcgagatc cgccgcggcg
     1561 acgatggcca ctaccactgg cctggccggg tcaacggccg cgcggtcgac ttcctgatcg
     1621 acaccggcgc gaccggcacc gcgatcccgg cgtcgctggc gcgcgagctc gagctcgtgt
     1681 cgctcggctc gatcagctcg aacaccgccg gcggcgtggt cagcgcgcag gtgatgcgcg
     1741 ccgacatcga gctgcagggc ggcgtcagcg tgcgccgact gcagatcgtc gcgctgccgc
     1801 ggctcggcga gaacccgctg cttgggatgg acgtgctcgg ccgcctgcac tggcagcagc
     1861 gcgatgcggt gctgcgcatc gagctcggca acggccgctg aacgtgccgc gcgccgcccg
     1921 gactgccatg acccgcctgc gccgcaccgc actgaccagc ctgctcgcac tgctgctggt
     1981 cggtgtcgcg cgtgcccagg ccgcgcccga gtgcccgccg agcgcgcagg tgccgacccc
     2041 cgagcaactc caggccgggg cgcgccaggc gcgcgaccat ggcttcctgt ggcgcatcag
     2101 caagggcggc cgcagctcct ggctctacgg cacgctgcat gtggcgaagc tcgaatggac
     2161 tttcccgggc ccggcggtga acgccgcgct gcgcgccagc gacacgatcg cgctcgagct
     2221 cgacgcgctc gaccccgagg tccagcgccg catggcccag gcgctggcgg cgcagacgca
     2281 cgcggcgctg ccgccggcgc tgcagcagcg cctggaccgc gctgcgcagg ccgaatgcgt
     2341 gcccgcccag gcgctggccg cgctcgcgcc ggagatgcag atcgcgacgc tgaccacgct
     2401 gctgggccgg cgcgatggcc tcgacgcgag ctacggcatc gacgttgtcc tcgccggctg
     2461 gggccacgcc ggcaagctcg aggtggtgtc gctcgaaacg ccggaactgc agctcaggac
     2521 cctgcagtcg gcgagcccgg ccgagaccgt cgaggcggtc gccagcgcgc tcgacgagct
     2581 cgaaggcggc cgcacgctgc cactgctgct gcgcatggcg cgggtctggg ccgacggcga
     2641 ctgggccgcg ctcacgggct acgccagctg gtgcgagtgc ctgaagaccg ccgccgaccg
     2701 cgccgagatg gcgcgtatgc tcgacgaacg taacccagcg ctggccgacg gcatcgcggc
     2761 gctgcacgcc cgcggccagc gcgtgttcgc cgcggtcggc agcctgcaca tgatcgggcc
     2821 gaccggtttg ccggcgctgc tcgcgcagcg cggttatcgt gtcgagcgca tcacttacaa
     2881 ccagacttcg gagacgcccc catgaccgac ccgtccgcct tttcgaagct cgtgcccggc
     2941 ttcgacttcc tgcaagggct cgtgaagaac gccggcgccg cgctgcccgg catcggctcc
     3001 tgggtcgcgc cgacgatcga cccggccgaa ctcgagaagc gcatcgagga gctgcgcacc
     3061 gtgcagttct ggctcgagca gaacgcgcgc atgctgggcg cgacgatcca ggcgctcgag
     3121 gtgcagcgca tgaccttgtc gacgctgaag acgatgaacg tgcagatgga ggacctgcgc
     3181 gattcgctgc agatccggat gccgacgatg ccgccgcccg ctgcgccgga acccgcgccc
     3241 gcggcgaagg ccacgcccgc gcccgatgcc gagcctgcac cgcccgcgcc gcccgcaggc
     3301 gccccggccg ggccggaggc ccgagcaggc gtcgtcgacc cgctgcagtg gtggggtgcg
     3361 ctgaccaagc agttcaccga actcgcgacc accgcgttga aggacaccgc ctccgacgcg
     3421 acccgcaagc tggccggcgc ggcgatgaag cgttcgctgg acccggccgg cgccgcgccg
     3481 gcggccccgg cgcgcagcgc cgctgcgaag aagaagaaaa ggcctgcagc gaagaaggcg
     3541 ccgcgcgcca aggcggcgcc gcacgattga tggcacgcgc gcgcaagcgt cattcctgag
     3601 cacgcggccc gcgccgacgc gccacgcccg acaattccgc ccaccatgaa ccgcttcctg
     3661 cacggccacg cgacgcatgc ggactggcgc gtcgcgctcg cgctggccgc ggcgcagatc
     3721 gaggcgcagc gccacacgcc cggtcatgcg gcgacgccga cgctgggctg ggtctacctg
     3781 accgatgcgc tggcgccgca cgccgaggcg ctgctcggtg aactgcgcca gcgctggccc
     3841 ggcgtcgaat gggtgggcgc gaccggcgtc ggcatcgccg cggccggcgt cgagtatttc
     3901 gacgcgccgg cactcgcgct gatgctgacc gatctggcgc cggcacgctt tcgcgtgttc
     3961 tcgggcgcgc ggccgctgac cggcttcgcg gcgcacaccg cgaacgtgca cgccgacccg
     4021 agcgccgccg acctggccga actgatccac gacatgagcg cgcgcaccgc gaccggctac
     4081 ctgttcggcg gcctcgcgtc ggcgccgtcg cggccgctgc acatcgccgg caatgccttg
     4141 gccggcggac tgtcgggcgt cgcgttcgac cgcggcgtcg cgctggtatc gcgcgtcacg
     4201 cagggttgcc agccggtcgg cccgcagcgc acggtgaccg ccgccgagcg caacgtggtc
     4261 atcgcgctgg acggcgaacc ggcgctcgac tgcctgctgc gcgacatcga cctgtccggt
     4321 gtcgacctgc gcagcgcgct gccgcgattg cgcgaggtgc tggtcggcct gagcgatgcc
     4381 ggcgacgccg ccgaagcgct cgcgccgcgc gaggacgcat cgacccgcag cgcgttcgac
     4441 gcgatcgcgc ggcaccgcag cacggcgcgc gcgttcggtg ccgacacgcg cgtgcggcat
     4501 ctggtcggcc tcgatccggc gcggcgcgcc atcgcgatcg ccgacagcgc cgagcccggc
     4561 gtgcggcttg cgttctgcgc ccgtcacgtc gaggcggcgc ggcgcgatct gatgcgcatc
     4621 tgcgccgagg tccgcgaaga ggtcgagacc gaggagttgc cgcaacgctc cgcgctcgca
     4681 ctgaaaggcg tgccgggcgc cgacgcgggc agcgccacgg cgtcgggcat cgccggcgcg
     4741 atctacgtca gctgctcggg gcgcggcggc gcgcacttcg gtgcgccctc ggccgaactg
     4801 ggcatcgtgc ggcacgcgct gggcgacgtg ccgctggtcg gcttcttcgc cgccggcgag
     4861 atcgcgcgcc gccatctcta cggctacacc ggcgtgctca ccgtgttccg gcgcggaggt
     4921 gacgccgcat gagcccgccg ggctgcccca agggcgaata ccggagtgcg cagcacgaag
     4981 gtactccagt gaacgccgca ctgcccgacg cgctgacgat cgaggtcgac gagcgcgcgc
     5041 gccgccagac cgaggtggtc gcggcgcttg cggccgtgtt gccggcgcac gcgctgctgt
     5101 ggcaccgcga ggacaccgtg ccctacgagt gcgacggcct gaccgcgtac cgcgagcagc
     5161 cgctcgtcgt ggcgctgccc gagaccgaag ccgaggtcgc ggccgtgctg cgcgaatgcc
     5221 atcggctgaa cgtgccggtg gtcgcgcgcg gcgccggcac cgggctatcg ggcggcgcgc
     5281 tgccgcaccg gctgggcgtg accctgagcc tggccaagtt caaccggatc ctgaagatcg
     5341 acccggtcgc gcgcacggcg ctcgtgcagt gcggcgtgcg caacctcgcg atcagcgagg
     5401 ccgcggcgcc ttacggcctg tactacgcgc ccgacccgag cagccagatc gcctgcacga
     5461 tcggcggcaa cgtcgccgag aactcgggcg gcgtgcactg cctgaaatac ggcctgacct
     5521 tgcacaacgt gctgcgcgtg cgcggcttca cggtcgacgg ctcggcggtc gagttcggct
     5581 ccgaggcgct cgactgcgcc gggctggacc tgatgacgat cctggtcggc agcgaaggca
     5641 tgctcgcggt gacgctcgag gtcagcgtca agctggtgcc caggccccaa ctcgcgcgct
     5701 gcatcatggc cagcttcgac gacatccgca aggccggcga cgcggtcgcc aacgtgatcg
     5761 ccgccggcat catcccggcc gggctggaga tgatggacaa gccgatgacc gcggcggtcg
     5821 aggactacgt gcacgccggc tacgacctgg ccgcgaaggc gatcatgctg tgcgagagcg
     5881 acggcacgcc cgaggaggtc gccgaagaga tcgcccgcat ggaggcggtg ctcggcacga
     5941 gcggtgcgac gcgcatcgag gtcagcgaca gcgaggcgca gcgcctgaag ttctggagcg
     6001 ggcgcaagaa cgcgttcccc gcatcgggcc gcatcagccc cgactacatg tgcatggact
     6061 cgacgatccc gcgccaccgg ctcgccgaca tcctgcttgc gatccaggcg atggagacga
     6121 agtacgggct gcgctgcgcg aacgtgttcc acgccggcga cggcaacctg cacccgctga
     6181 tcatgttcga tgccaacgac cccgaccagc tgcaccgctg cgagctgttc ggcgccgaca
     6241 tcctcgagtc cagcgtcgcg atgggcggca ccgtgtcggg cgagcatggc gtgggcatcg
     6301 agaagctctc ggcgatgtgc gtgcagttcg cgccggccga gcgcgaacag atgctggcgt
     6361 tgaaacgtgc gttcgatccg aaggagctgc tgaacccggg caaggcgatc ccgtcgctgg
     6421 cgcgctgtgc cgagtacggc aagatgcatg tgcggcgcgg gctgctgccg ttcgccgaca
     6481 ttccgcgctt ctgagctgaa tgagaaattc aaaccgatct ctgcgggccg agcgccgggc
     6541 cgctcccaag ccggccc
//
LOCUS       DATMJB010000423         2169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_479237, whole genome shotgun sequence.
ACCESSION   DATMJB010000423 DATMJB010000000
VERSION     DATMJB010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479237"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..>2169)
                     /locus_tag="VNU48_09135"
     CDS             complement(19..>2169)
                     /locus_tag="VNU48_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWI11478.1"
                     /translation="LFLNSWRSTVITGLTLPIALIGTFLFMYAFGFTINSITMMALSL
                     CVGLLIDDAIVVRENIVRHVQMGKSAHQAALDGTNEIGLAVLATTCSIVAVFLPIGFM
                     GGIVGKFFHEFGLTIVAAVLISMFVSFTLDPMLSSIWHDPQAHGGPRGKPVTRYDRTI
                     GRVTLLFDRLTDVLSRAYQRILRWSLRHRLATLAIALASFVGSFFVIPLLGAEFVPKA
                     DFSETQINFYTPVGSSLELTEARARQVDAALRELPEVKYTVTTINSGQAAGKIYAAVY
                     VRLVDRKDRARSIDQMAVPLRARLARIPGITITNIGVTDLGGGKSLQFSVQGPDLAEL
                     ERLSRMITPKLLAIRGLVDLDSTLKPDKPTVSIDVKRDAASDVGLNVNMLATSLRTMV
                     AGTLVGNWRAPDGENYDVNVRLAPQSRVAIGDLQRMPINVAAAADGSARSVRLSQVAD
                     ITASTGPNQINRRDLNREINFDANAFGRSSGEVSAEIKAALDAVAWPPGYRYSFGGST
                     KNMNESFQYAVGALALAIVFIYMILASQFKSFLQPLALMSALPLTLIGVVLTLLVFRS
                     TLNMFSIIGVVMLMGLVTKNAILLIDFAIRARHGEHAGEHGLDHDVAPLGREAALLLA
                     ARVRLRPILMTTLAMVFGMVPLAFALSEGSEQRAPMGQAVIGGVITSSLLTLVVVPVI
                     YCYLDDLGAWARRRFGSTTPAPAADASLAATPSK"
BASE COUNT          352 a          727 c          762 g          328 t
ORIGIN      
        1 ggaaggcgcg cgcggattct atttcgaggg ggtcgcggcg aggctcgcat cggcggccgg
       61 cgccggggtc gtcgatccga agcgccgcct tgcccaggcg cccaggtcgt cgaggtagca
      121 gtagatcacc ggcacgacca ccagcgtcag cagcgacgag gtgatcacgc cgccgatcac
      181 cgcctggccc atcggcgcgc gctgctccga gccttcggac agcgcgaacg cgagcggcac
      241 catgccgaag accatcgcca gcgtcgtcat caggatcggg cgcagccgca ccctggcggc
      301 aagcagcagc gcggcttcgc ggcccagcgg cgccacgtcg tggtcgaggc cgtgttcgcc
      361 ggcatgctcg ccgtggcgcg cgcgaatcgc gaagtcgatc agcaggatcg cgttcttcgt
      421 caccagcccc atcagcatca cgacgccgat gatgctgaac atgttcagcg tcgagcggaa
      481 caccagcagc gtcagcacga cgccgatcag cgtcagcggc agcgcgctca tcagcgcgag
      541 cggctgcagg aagctcttga actggctcgc caggatcatg tagatgaaca cgatcgccag
      601 cgccagcgcg cccacggcgt actggaacga ctcgttcatg ttcttggtcg agccgccgaa
      661 gctgtagcgg tagccgggcg gccaggcgac ggcgtcgagc gcggccttga tctcggccga
      721 cacctcgccc gagctgcggc cgaacgcatt cgcgtcgaag ttgatctcgc ggttcaggtc
      781 gcggcggttg atctggttcg ggccggtcga cgccgtgatg tcggccacct gcgacagccg
      841 caccgagcgc gccgagccgt cggcggccgc cgcgacgttg atcggcatgc gctgcaggtc
      901 gccgatcgcg acgcggcttt gcggcgcgag acgcacgttg acgtcgtagt tctcgccgtc
      961 gggcgcgcgc cagttgccga ccagcgtgcc cgccaccatc gtgcgcaggc tggtcgcgag
     1021 catgttgacg ttcaggccaa cgtcggaggc ggcgtcgcgc ttcacgtcga tcgacacggt
     1081 cggcttgtcg ggcttgagcg tgctgtccag gtcgaccagg ccgcggatcg ccagcagctt
     1141 gggcgtgatc atgcgcgaca ggcgctcgag ctcggccagg tccgggccct gcaccgagaa
     1201 ctgcaggctc ttgccgccgc ccaggtcggt gacgccgatg ttggtgatcg tgatgcccgg
     1261 gatgcgcgcg agccgcgcgc gcagcggcac cgccatctgg tcgatgctgc gcgcgcggtc
     1321 cttgcggtcg accaggcgca cgtagaccgc ggcgtagatc ttgccggcgg cctggccgct
     1381 gttgatcgtc gtgaccgtgt acttcacttc gggcagctcg cgcagcgcgg cgtcgacctg
     1441 ccgggcgcgc gcctcggtca gctccagcga cgagcccacc ggcgtgtaga agttgatctg
     1501 cgtctcggag aagtcggcct tcggcacgaa ctcggcgccg agcagcggga tcacgaagaa
     1561 gctgccgacg aagctggcca gcgcgatcgc gagcgtggcc agccggtgcc gcagcgacca
     1621 gcgcaggatg cgctggtagg cgcgcgacag cacgtcggtg agccggtcga acagcagcgt
     1681 gacgcggccg atggtgcggt cgtagcgcgt cacgggtttg ccgcgcggcc cgccgtgcgc
     1741 ctgcgggtcg tgccagatgc tggagagcat cgggtccagc gtgaaactga cgaacatcga
     1801 gatcagcacc gccgcgacga tcgtgaggcc gaactcgtgg aagaacttgc cgacgatccc
     1861 gcccatgaag ccgatcggca ggaacaccgc gacgatcgag caggtcgtcg cgagcaccgc
     1921 caggccgatc tcgttggtgc cgtccagcgc cgcttggtgc gcgctcttgc ccatctgcac
     1981 atggcgcacg atgttctcgc gcaccacgat cgcgtcgtcg atcagcaggc ccacgcacag
     2041 gctcagcgcc atcatcgtga tgctgttgat cgtgaagccg aacgcgtaca tgaacaggaa
     2101 cgtgccgatc agcgcgatcg gcagcgtcag gcccgtgatg acggtggagc gccacgagtt
     2161 caggaacag
//
LOCUS       DATMJB010000424         2188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_524564, whole genome shotgun sequence.
ACCESSION   DATMJB010000424 DATMJB010000000
VERSION     DATMJB010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524564"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VNU48_09140"
     CDS             complement(<1..644)
                     /locus_tag="VNU48_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWI11479.1"
                     /translation="MTGPKNKPKPGTRTPAATGASDALVRRQRHRATAAADAAMPRRA
                     LALLARLGRHLHEPRDPHALHELVVTQTVSLTGAQRVLLVLEAPAGRRLAGARVPTGE
                     APDALLRAIAPWLDAARHSRAAALRHGPDGAAAAAQRSCLVTPLIRRRELLGYLYADI
                     DGTHGRFTDAQRDLLALLADQAAAALANLRAIAVLDAKVAERSAELEQRSDALAL"
     gene            complement(678..2066)
                     /locus_tag="VNU48_09145"
     CDS             complement(678..2066)
                     /locus_tag="VNU48_09145"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWI11480.1"
                     /translation="MTAPSICRPNARRGALRAMGGVVLLFGPFDIAFGASIVAVRVWP
                     AADYTRVTIESDAALSQKHFLAENPNRLVIDIDGLELSPALRDLVGKVRADDPFIAGV
                     RAGQNQPRVVRVVIDLKQPTAPQLFTLAPVAAYQHRLVFDLYPTQERDPLLALIRDKE
                     AAEQQAAKSVQDALGEFIGKMGKPAEPPAALPPLAREPGPTSPPIATAPPPQQTPAPL
                     PPAVQGKIDRLIVVAIDPGHGGEDPGASGPSGLHEKDVVLQIALQLRDRINTLPNMRA
                     MLTRDADFFVPLHDRVRKAQRVQADLFVSIHADAFITPAARGASVFALSQNGATSAAA
                     RWMANRENAADAIGGVNIKAKDASVLRALLDMSTTAQIRDSLKLGGEVLGQIGRVGKL
                     HKGSVEQAGFAVLKAPDIPSILVESAFISNPEEEMRLRDPQYQAQLVNALAHGIQRYF
                     AKNPPLARSRQL"
BASE COUNT          271 a          758 c          825 g          334 t
ORIGIN      
        1 agcgccagcg cgtcgctgcg ctgttcgagt tcggcgcttc gttcggcgac cttggcgtcg
       61 agaaccgcga tcgcgcgcag gttggccagc gccgccgcgg cctggtcggc gagcagcgcc
      121 agcaggtcgc gctgggcatc ggtgaagcgg ccgtgcgtgc cgtcgatgtc ggcgtacagg
      181 tagcccagca gttcacgccg cctgatcagc ggcgtgacca ggcaggagcg ctgggccgcc
      241 gcggcggcgc cgtccgggcc gtggcgcagc gcggccgcgc ggctgtgtcg cgcggcatcg
      301 agccacggtg cgatcgcgcg cagcagggcg tcgggcgcct cgccggtcgg gaccctcgcg
      361 ccggccaggc gccgcccggc cggtgcctcc agcaccagca gcacgcgttg cgcgccggtg
      421 agcgacaccg tctgcgtgac cacgagttcg tgcagcgcgt gagggtcacg cggctcgtgc
      481 aggtgtcggc ccaggcgtgc cagaagcgcc agggcgcggc gcggcatcgc cgcgtcggcg
      541 gcggccgtgg cgcgatggcg ctggcgcctg accagggcat cgctcgctcc ggtcgcggca
      601 ggcgttcggg tccccggctt gggcttgttc ttcggaccag tcatgggctg ctcgatacgg
      661 aacgtgctcg atcgacgcta caactggcgc gagcgcgcca gcggcgggtt cttcgcgaag
      721 tagcgctgga tgccgtgcgc cagcgcattc acgagctgcg cctggtattg cgggtcgcgc
      781 aggcgcatct cttcctcggg gttcgagatg aacgccgact cgaccaggat cgacgggatg
      841 tcgggcgcct tcagcaccgc gaaaccggcc tgctcgacgc tgcccttgtg cagcttgccg
      901 acccggccga tctggcccag cacctcgccg ccgagcttga ggctgtcgcg gatctgcgcg
      961 gtcgtgctca tgtcgagcag cgcgcgcagc acgctcgcgt ccttggcctt gatgttgacg
     1021 ccgccgatcg cgtcggcggc gttctcgcgg ttcgccatcc agcgcgccgc ggcgctggtg
     1081 gcgccgttct ggctgagtgc gaacaccgac gcgccgcgcg ccgccggcgt gatgaacgcg
     1141 tcggcgtgga tcgagacaaa gagatcggcc tgcacgcgct gtgccttgcg cacgcggtcg
     1201 tgcagcggca cgaagaagtc ggcgtcgcgc gtcagcatcg cgcgcatgtt cggcaaggtg
     1261 ttgattcgat cgcgcagctg cagcgcgatc tgcagcacca cgtccttctc gtgcaggccg
     1321 ctcgggccgc tcgcgccggg gtcttcgccg ccgtggccgg ggtcgatcgc gacgacgatg
     1381 agccggtcga tcttgccctg caccgcgggc ggcagcggcg ccggggtctg ctgtggcggc
     1441 ggcgcggtcg cgatcggcgg cgatgtcggc ccgggctcac gcgccagcgg cggcagggcc
     1501 gcggggggct cggcgggttt gcccatcttg ccgatgaact cgccgagcgc gtcctgcacc
     1561 gacttcgcgg cctgctgttc ggcggcctcc ttgtcgcgga tcagcgccag cagcgggtcg
     1621 cgttcctgcg tcgggtacag gtcgaacacc aggcggtgct ggtacgcggc gaccggcgcg
     1681 agcgtgaaca gctgcggcgc ggtcggctgc ttcaggtcga tcacgacacg caccacgcgc
     1741 ggctggttct ggccggcgcg cacgccggcg atgaacgggt cgtcggcgcg caccttgccg
     1801 accaggtcgc gcagcgccgg gctgagctcg agaccatcga tgtcgatcac gagccggttc
     1861 gggttctcgg ccaggaagtg cttctgcgac agcgccgcgt cggactcgat cgtcacgcgg
     1921 gtgtagtcgg ccgccggcca gacgcgcacc gcgacgatgc tggcgccgaa tgcgatgtcg
     1981 aagggcccga acagcagcac cacgccgccc atcgcgcgca gcgcgccgcg gcgcgcgttg
     2041 gggcggcaga tcgatggtgc cgtcatgtca gttgccccac cgcatctcgc gcgacccccg
     2101 acacatgagg atgtgcaagc gcagggggcc gcttgcggcc cgttgcagac gctcgaactt
     2161 ggtgtgcgtc cgtcggcgcg gcaaaggc
//
LOCUS       DATMJB010000425         2542 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_190806, whole genome shotgun sequence.
ACCESSION   DATMJB010000425 DATMJB010000000
VERSION     DATMJB010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190806"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..555
                     /locus_tag="VNU48_09150"
     CDS             52..555
                     /locus_tag="VNU48_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWI11481.1"
                     /translation="MVFGAVAGIAAAAGAGLAWRRDRPAAAPAAEFWTLRFDQPGGGQ
                     LGMAAFRGRPLLLNFWATWCPPCVSELPLLDRFFRDQPRDGWQVAGLAVDNLAPVLTF
                     LAQHPVTFPIGLAGTDGIELARRLGNSSGALPYTAVFDRSGALVQRKLGVIEAADLQK
                     WVAAAAG"
     gene            838..1296
                     /gene="accB"
                     /locus_tag="VNU48_09155"
     CDS             838..1296
                     /gene="accB"
                     /locus_tag="VNU48_09155"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HWI11482.1"
                     /translation="MDLRKLKTLIDLVSESNISELEITEADGKVRIVKADPAAPMPVH
                     YVPAPTMVAAPAPATAASPSAAEPPAEPVETGHVVKSPMVGTFYRAANPGSKPFAEIG
                     DAIKEGDPICIIEAMKIMNEIEADTSGTVTRILCENGQAVEFGQPLFVIG"
     gene            1419..>2542
                     /gene="accC"
                     /locus_tag="VNU48_09160"
     CDS             1419..>2542
                     /gene="accC"
                     /locus_tag="VNU48_09160"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWI11483.1"
                     /translation="MFKKILIANRGEIALRVQRACRELGIKSVIVYSEADRDAKYVKL
                     ADEAVCIGPASSAQSYLNMPAIISTAEVTDAEAIHPGYGFLSENADFAERVERSGFTF
                     IGPTPASIRVMGDKVSAKQAMIKSGVPCVPGSEGALPEDPKEIIKAARAVGYPVIIKA
                     SGGGGGRGMRVVHTEAALIHAVQTTRAEAGAAFGNPAVYMEKFLENPRHIEIQVLADE
                     HKNAVWLGERDCSMQRRHQKVLEEAPAPGIPRRVIERIGERCAAACKKLGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELVTGIDIVQMQIRIAAGEKLPFTQRNIQMRGHA
                     IECRINAEDPYKFTPSPGRITMWHAPGGPGVRVDSHAYTN"
BASE COUNT          450 a          831 c          874 g          387 t
ORIGIN      
        1 ggcgaatccc gcagtgcaca gcacggagct tgcctgatga gccggcggcg catggtgttc
       61 ggcgcggtcg ctgggatcgc ggcggcggct ggcgcgggtc tggcctggcg gcgcgatcgc
      121 ccggcggccg cgcccgctgc cgagttctgg accctgcgct tcgatcaacc cggtggcggg
      181 caactcggga tggccgcgtt ccgcggccgg ccgctgctct tgaacttctg ggccacctgg
      241 tgtccgccct gcgtttcgga gctgccgttg ttggatcgct tcttccgcga tcaaccgcgc
      301 gacggttggc aggtcgcggg ccttgccgtg gacaacctgg ccccggtcct gacgttcctg
      361 gcccagcacc cggtcacgtt cccgatcgga ctggcgggca cggacggcat cgagctggcg
      421 cggcgactgg gcaacagcag cggcgcgctg ccctacacgg cggtgttcga ccgcagcggc
      481 gcgctggtgc agcgaaaact gggcgtgatc gaggcggcgg acctgcagaa gtgggtcgcc
      541 gcggcggcgg gctgagcagg cgccgcaaga cgccaacgca tttcggcaac gcggattttc
      601 gtcttgtcaa cagcacgcaa tttcgcacaa gtcgcgtaaa gtaccctctt aattgttgtg
      661 gacgtgcaaa gcgagcgagt cgggtggccg gcatgccgct tcttgacgag ttcttgactg
      721 gatcgattgt cttgaacaga tgacgacgcg acgcggcgag atcggcgcag tatcagcgcc
      781 ggccgcggca ccagcagttc tcgcgcgccg ccacaccccg acaggacttg atacaccatg
      841 gatcttcgaa aactcaagac actcatcgat ctggtttccg agtcgaacat ctcggagctg
      901 gagatcaccg aagccgacgg caaggtccgc atcgtcaagg ccgatccggc cgcgccgatg
      961 ccggtccact atgtgcccgc gccgaccatg gtcgcggcgc ccgcgccggc gacggccgcg
     1021 tcaccgtccg ccgcggaacc ccctgcggaa cccgtcgaga ccggccatgt cgtcaagtcg
     1081 ccgatggtgg gcaccttcta ccgcgccgcc aacccgggat cgaagccgtt cgccgaaatc
     1141 ggcgacgcga tcaaggaagg cgacccgatc tgcatcatcg aggcgatgaa gatcatgaac
     1201 gagatcgagg ccgacacgtc ggggacggtc acgcgcatcc tgtgtgagaa cggtcaggcc
     1261 gtggagttcg ggcaacccct gttcgtcatc ggataaggcc ggcccatgtt caagaaaatc
     1321 ctcattgcca atcggggcga aaacgccgcg tgcggcgttt cggcgcagcc acaccgcgcg
     1381 gcgagcgaag cgcgccggcg cgcttgagcc gaggcatcat gttcaagaaa attctgattg
     1441 caaaccgagg cgagatcgcg ctgcgggtgc agcgcgcctg ccgcgagctg ggcatcaagt
     1501 cggtcatcgt ctactccgag gccgaccgcg acgcgaagta cgtgaaactg gccgacgagg
     1561 ccgtgtgcat cgggccggcg agttcagcgc agagctacct gaacatgccg gcgatcatct
     1621 cgaccgccga agtcaccgat gccgaggcga tccatccggg ctacggcttc ctctcggaga
     1681 acgccgactt cgccgagcgc gtggaacgca gcggcttcac cttcatcggg ccgacgccgg
     1741 cctcgatccg cgtgatgggc gacaaggtgt cggccaagca ggcgatgatc aagtccggtg
     1801 tgccctgcgt gccgggatcg gaaggcgcgc tgccggaaga cccgaaggag atcatcaagg
     1861 cggcgcgcgc ggtcggctac ccggtgatca tcaaggcctc gggcggcggc ggcgggcgcg
     1921 gcatgcgggt ggtccacacc gaagcggcgc tgatccatgc ggtgcagacg acgcgcgccg
     1981 aggccggcgc cgcgttcggc aatccggccg tgtacatgga gaagttcctc gagaacccgc
     2041 gccacatcga gatccaggtg ctcgccgacg aacacaagaa cgcggtctgg ctgggcgagc
     2101 gcgattgctc gatgcagcgc cggcaccaga aggtcctgga agaggcgccc gcgccgggca
     2161 ttccgcggcg cgtgatcgag cgcatcggcg agcgctgtgc cgcggcctgc aagaagctcg
     2221 gctaccgcgg cgccggcacc ttcgagttcc tgtacgaaga cggcgagttc ttcttcatcg
     2281 agatgaacac gcgcgtgcag gtcgagcacc cggtcacaga gctcgtgacg ggcatcgaca
     2341 tcgtgcagat gcagatccgc atcgccgcgg gcgagaagct gccgttcacg cagcgcaaca
     2401 tccagatgcg cgggcacgcg atcgagtgcc ggatcaacgc cgaggacccc tacaagttca
     2461 cgccgtcgcc gggccgcatc acgatgtggc acgcgccggg agggccgggc gtgcgggtgg
     2521 actcgcacgc gtacacgaac ta
//
LOCUS       DATMJB010000426         2862 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_798502, whole genome shotgun sequence.
ACCESSION   DATMJB010000426 DATMJB010000000
VERSION     DATMJB010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798502"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1762
                     /locus_tag="VNU48_09165"
     CDS             71..1762
                     /locus_tag="VNU48_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI11484.1"
                     /translation="MPIPDSVPTEALALQRLYHWERHAPARVVLTQPLGGGATRDYTW
                     AQLIDESRRMAAHLQSLGFEPGSRIAILSKNCAHWLMADFAIWMAGFVSVPLYPTLAT
                     GTIRQILEHSEARLLFIGKLDGWGAMKPGVPAGLPCIAMPLAPPVDCAQWQAIVAAIA
                     PLRGEPVRDGAELATIMYTSGTTGSPKGVMHSFATFGWSMQAALARVGLESDARMLSY
                     LPLAHVAERTLVEHGLLATSMRVFFAESLETFGADLQRARPTCFFSVPRLWVKFQQGV
                     HAKISPAKLERLLKIPILRRIVAKKVLGAIGLDQCALAAGGAAPMPPELLAWYASLGL
                     PIVEVYGMTENCGVSHATLRGVQRPGTVGKAYDGVQSRIDTRSGEIQMKSPGLMLGYY
                     KEPGLTRDALTADGWLHTGDKGQLDAEGNLRITGRVKDLFKTSKGKYVAPAPIEDQLV
                     MNGAVEACVVTGANLGQPLGIVMLNAEAAQRAQDAGQRQAMEASLTAHLDAINARLDP
                     HEQLDCLVVVSDPWTVDNGFITPTFKVKRNRIEEVYGALYERWVGAHRRVVWHPS"
     gene            1845..2828
                     /locus_tag="VNU48_09170"
     CDS             1845..2828
                     /locus_tag="VNU48_09170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWI11485.1"
                     /translation="MTDISTRPARPAISCIVPAYNEAPNLGPLLTGLTVELAGLTERW
                     EIVVVDDGSRDATPAVMAPWLATPGVRYLRLSRNFGKEAALTAGIDHAVGDVVLLMDA
                     DLQHPASLVPEMVKAWQDGADMVCAARASRADESWAKRLGTALFYRLVNHNAAVPIPV
                     DAGDFRLMDRRVVDALKALPERNRFMKGLYAWVGFHNVIIPYVPAERHAGTSSFSLRR
                     LSKLAFTGVTAFTNAPLRLWSAIGVLVALFALGFGMWIVIEYFIYGADVPGWATLACG
                     VMFFSGVQLISIGILGEYVGRIFDEVKQRPVYLIGSEAGRGAIHVPSEPHA"
BASE COUNT          423 a         1032 c          994 g          413 t
ORIGIN      
        1 agtcccactc ttggcgtcgc cgcgcgcatg ccataaggtc agcgacaagc ccccttcgag
       61 gccacgcccg atgccgatcc ctgactccgt gccgaccgag gcgctggcgc tgcagcgcct
      121 gtaccactgg gaacgccacg cgcccgcgcg cgtcgtgctg acgcagccgc tgggcggcgg
      181 cgcgacgcgc gactacacct gggcgcagct gatcgacgag tcgcgccgca tggccgcgca
      241 cctgcagtcg ctcggcttcg agccgggcag ccgcatcgcg atcctgtcga agaactgcgc
      301 gcactggctg atggccgact tcgcgatctg gatggccggc ttcgtctcgg tgccgttgta
      361 ccccaccctg gccaccggta cgattcgcca gatcctcgag cacagcgagg cccgcctgct
      421 gttcatcggc aagctcgacg gctggggcgc gatgaagccc ggcgtgccgg ccgggctgcc
      481 ctgcatcgcg atgccgctgg caccgccggt cgactgcgcg cagtggcagg cgatcgtcgc
      541 cgccatcgcg ccgctgcgcg gcgaaccggt gcgcgatggc gccgagctcg cgaccatcat
      601 gtacacctcg ggcacgaccg gcagccccaa gggcgtgatg cacagcttcg caaccttcgg
      661 ctggtcgatg caggcggcgc tggcgcgcgt cggcctcgaa agcgacgcgc gcatgctgtc
      721 gtacctgccg ctggcccacg tcgccgagcg cacgctggtc gagcacggcc tgctcgcgac
      781 ctcgatgcgc gtgttcttcg ccgagagcct ggagaccttc ggcgccgacc tgcagcgcgc
      841 gcgcccgacc tgcttcttct cggtgccgcg cctgtgggtc aagttccagc agggcgtgca
      901 cgcgaagata tcgccggcca agctggagcg gctgctgaag atcccgatcc tgcggcgcat
      961 cgtcgcgaag aaggtgctcg gcgcgatcgg cctggaccag tgcgcgctcg cggccggcgg
     1021 cgctgcgccg atgccgcccg agctgctcgc ctggtacgcg agcctgggcc tgccgatcgt
     1081 cgaggtctac ggcatgaccg agaactgcgg tgtctcgcac gccaccttgc gcggcgtgca
     1141 gcggcccggc accgtcggca aggcctacga cggcgtgcaa agccggatcg acacgcgcag
     1201 cggcgagatc cagatgaaga gccccggcct gatgctgggc tactacaagg agcccgggct
     1261 gacgcgcgat gcgctcaccg ccgacggctg gctgcacacc ggcgacaagg gccagctcga
     1321 cgccgagggc aacctgcgca tcaccggccg cgtcaaggac ctgttcaaga ccagcaaggg
     1381 caagtacgtc gcacccgcgc cgatcgagga ccagctggtg atgaacggcg cggtcgaggc
     1441 ctgcgtcgtc accggcgcca acctcggcca gccgctgggc atcgtgatgc tcaatgccga
     1501 ggccgcccag cgtgcgcagg acgccggcca gcgccaggcg atggaggcgt cgctcacggc
     1561 gcacctggac gcgatcaacg cccggctcga cccgcacgag cagctcgatt gtctcgtggt
     1621 cgtcagcgac ccgtggacgg tcgacaacgg tttcatcacg ccgaccttca aggtcaagcg
     1681 caaccgcatc gaagaggtct acggcgcgct ctacgagcgc tgggtcgggg cacaccgacg
     1741 cgtggtctgg cacccgagct gacggcgcgg tcgcaggcgc ggcccgggcc ggcggcgtgc
     1801 ggccggcggg cagcgtaaag tcgcgccgga acacgcgctt gcccatgacc gacatctcga
     1861 cccgacccgc ccgccccgcg atcagctgca tcgtgccggc ctacaacgaa gcgccgaacc
     1921 tcggcccgct gctgaccggc ctgaccgtcg agctcgccgg cctgaccgag cgctgggaga
     1981 tcgtcgtcgt cgacgacggc agccgcgacg cgacgccggc ggtgatggcg ccctggctcg
     2041 cgacgcccgg cgtgcgttat ctgcggctgt cgcgcaactt cggcaaggag gccgcgctga
     2101 ccgccggcat cgaccacgcg gtcggcgatg tggtgctgct gatggacgcc gacctgcagc
     2161 acccggcctc gctggtgccc gagatggtca aggcctggca ggacggtgcc gacatggtct
     2221 gcgccgcgcg cgcctcgcgc gccgacgaga gctgggccaa gcgcctgggt acggcgctgt
     2281 tctaccggct ggtgaaccac aacgccgcgg tgccgatccc ggtcgatgcg ggcgacttcc
     2341 ggctgatgga ccggcgcgtc gtcgatgcgc tgaaggcact gcccgaacgc aaccgcttca
     2401 tgaaggggct gtacgcctgg gtcggcttcc acaacgtgat cattccgtac gtgccggccg
     2461 agcgccacgc cggcaccagc agcttctcgc tgcgccgcct gtcgaagctc gcgttcaccg
     2521 gcgtcaccgc attcaccaac gcaccgctgc ggctgtggag cgcgatcggc gtgctggtcg
     2581 cgctgttcgc gctcggcttc ggcatgtgga tcgtgatcga gtacttcatc tacggcgccg
     2641 acgtccccgg ctgggccacg ctggcgtgcg gcgtgatgtt cttcagcggc gtgcagctga
     2701 tctcgatcgg catcctcggc gagtacgtcg gccgcatctt cgacgaggtc aagcagcggc
     2761 cggtctacct gatcggcagc gaggcggggc gcggggcgat tcacgtgccg agcgaaccgc
     2821 acgcatgagc cgggcgccga gccgcctcaa gcccggctgc gc
//
LOCUS       DATMJB010000427         2401 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_656944, whole genome shotgun sequence.
ACCESSION   DATMJB010000427 DATMJB010000000
VERSION     DATMJB010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656944"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VNU48_09175"
     CDS             <1..451
                     /locus_tag="VNU48_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWI11486.1"
                     /translation="RDLAPPPPPPGQRVKLMPASVARRVYEEHQAFYDKLQADVAAAA
                     RKAFVDPDAALAQVGAAAEATALVGKFNPAVVLISGCQDNQTSMDGEHNGAFTEQLLK
                     VWNRGGFNGDYDAFHARIRAGMPHSQSPNLFVLGKAANFLKQKPFTV"
     gene            456..>2401
                     /locus_tag="VNU48_09180"
     CDS             456..>2401
                     /locus_tag="VNU48_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11487.1"
                     /translation="MPARSVPIRLVGREADRKLPALLTPASRTVGRSDDVFLPSGYLV
                     PTAAFDVGPVARDVGEAQHQSHDAGADEVVVLELADGSTLITSAQRLRASLQQTHPEL
                     VGDDGAILLEQLRTIGAAPGRGFGEAVGGLISKVYSFAVGAAPDAIVDEALSQLPNPL
                     ELGVSWLGTKLLMAAIEKRLDQPPGLYRWVGASGQRSDLAPVDLNDPALPAPSAERPM
                     LVFVHGTGSNTLGSFGDLRTGDRDLWGMLAARYTGGIFAFEHRTLSQSPIENAIALAS
                     ALPRGAQLSLVSHSRGGLVADLLCLGDFDALIEHYAYAFDGTGDTDPAEAQRVLGELK
                     NAHAEQRTQLRTLAGVLRERAITVQRYVRAASPASGTLLASGNFDVFLSGILSLIGLV
                     PFFFGSPVYSAFKRVVVEVAKNRTNAHLVPGIEAMLPDSPMARLLRDAPVRPGTAMAV
                     IAGDIQGGNLLRRLGVMLTDFLLFDNEDNDLVVNTSSMLAGIAPKSDARVLFDRGADV
                     SHFRYFSNIDTRSALRDWLIDPDTRTLTMFRALPTPAEFAQALAASAASRDAEAADRP
                     VVVVLPGVMGSHLQAAGGDRVWFDPLDIASGGLSKIEWGQPSVEAEALFAMFYGKVCQ
                     YLADSHRVEPFPYDWRQPLDVLAER"
BASE COUNT          343 a          883 c          830 g          345 t
ORIGIN      
        1 gcgcgacctc gcgccgccac cgccgccgcc cggtcagcgc gtcaagctga tgccggcgag
       61 cgtggcgcgc cgcgtctacg aggagcacca ggccttctac gacaagctgc aggccgacgt
      121 ggccgcggcc gcgcgcaagg ccttcgtcga ccccgacgcg gcgctcgcgc aggtcggcgc
      181 cgcggccgag gcgaccgcgc tggtgggcaa gttcaacccg gcggtcgtgc tgatctcggg
      241 ctgccaggac aaccagacct cgatggacgg cgagcacaac ggcgcgttca ccgagcagct
      301 gctgaaggtc tggaaccgcg gcggcttcaa tggcgactac gacgcgttcc acgcgcgcat
      361 ccgcgccggc atgccgcaca gccagtcgcc gaacctgttc gtgctcggca aggccgcgaa
      421 cttcctgaag cagaagccgt tcacggtctg acgacatgcc cgcccgcagc gtcccgatcc
      481 gcctcgtcgg ccgcgaggcc gatcgcaagc tgccagcgct gctgacgccg gcgtcacgca
      541 ccgtcggccg cagcgacgac gtgttcctgc cttcgggcta tctggtgccg accgccgcgt
      601 tcgatgtcgg cccggtggcg cgcgatgtcg gcgaggcgca gcaccagtcg cacgacgcag
      661 gcgccgacga agtggtcgtg ctcgagctcg ccgacggcag cacgctgatc acgagcgcgc
      721 agcggctgcg cgcctcgctg cagcagacgc accccgagct ggtcggcgac gacggcgcga
      781 tcctgctcga acaactgcgc accatcggcg ccgcgccggg ccgcggcttc ggcgaggccg
      841 tcggtgggct gatcagcaag gtctacagct tcgccgtcgg tgccgcgccc gacgcgatcg
      901 tcgacgaggc gctgagccag ttgccgaacc cgctcgaact cggcgtgagc tggctcggaa
      961 cgaagctgct gatggcggcg atcgagaagc gcctcgacca gccgccgggc ctgtaccgct
     1021 gggtcggcgc gagcggtcag cgcagcgatc tcgcaccggt cgacctgaac gacccggcgc
     1081 tgcccgcgcc gtcggccgag cgcccgatgc tggtgttcgt gcacggcacc ggctcgaaca
     1141 cgctgggcag cttcggcgac ctgcgaaccg gcgaccgcga cctctggggc atgctggccg
     1201 cgcgctacac cggcggcatc ttcgcgttcg agcaccgcac gctgtcgcaa agcccgatcg
     1261 agaacgcgat cgctctcgcg agtgcgctgc cgcgcggcgc gcagctgagc ctggtgtcgc
     1321 attcgcgcgg cgggctcgtc gccgacctgc tgtgcctggg cgacttcgac gcgctgatcg
     1381 agcattacgc ctacgcgttc gacggcaccg gtgacaccga tccggccgag gcgcagcgcg
     1441 tgctgggcga attgaagaac gcgcacgccg agcagcgcac gcagctgcgc acgctcgccg
     1501 gcgtgctgcg cgagcgtgcc atcaccgtgc agcgctacgt gcgcgccgcg agcccggcga
     1561 gcggcacgct gctggcgagc ggcaacttcg acgtgttcct gtcgggcatc ctgagcctga
     1621 tcggcctggt gccgttcttc ttcggcagcc cggtctactc ggcgttcaag cgtgtcgtcg
     1681 tcgaggtcgc gaagaaccgc accaacgcac acctcgtgcc cggcatcgag gcgatgctgc
     1741 ccgactcgcc gatggcgcgg ctgctgcgcg acgcgccggt gcgccccggc accgcgatgg
     1801 ccgtgatcgc cggcgacatc cagggcggca acctgctgcg ccgcctgggc gtgatgctga
     1861 ccgacttcct gctgttcgac aacgaagaca acgacctcgt cgtcaacacc agctcgatgc
     1921 tcgccggcat cgcgccgaaa tcggacgcgc gcgtgctgtt cgatcgcggc gccgatgtgt
     1981 cgcacttccg ctacttcagc aacatcgaca cgcgcagcgc gctgcgcgac tggctgatcg
     2041 accccgacac gcgcaccctg acgatgttcc gcgcgttgcc gacgccggcc gagttcgcgc
     2101 aggcgctggc ggcctcggcg gcgagccgcg acgccgaggc cgcggatcgt ccggtcgtcg
     2161 tcgtgctgcc cggcgtgatg ggctcgcacc tgcaggccgc cggcggtgac cgggtctggt
     2221 tcgatccgct ggacatcgcc tccggtggcc tgtccaagat cgaatggggc cagccctcgg
     2281 tcgaggccga ggcgctgttc gcgatgttct acggcaaggt ctgccagtac ctcgccgaca
     2341 gccaccgcgt cgagcccttc ccctacgact ggcgccagcc gctcgacgtg ctggccgagc
     2401 g
//
LOCUS       DATMJB010000428         4335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_286112, whole genome shotgun sequence.
ACCESSION   DATMJB010000428 DATMJB010000000
VERSION     DATMJB010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4335
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286112"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VNU48_09185"
     CDS             <1..190
                     /locus_tag="VNU48_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11488.1"
                     /translation="ASLDSVQRALNERCGAFVPGSLRVADLRHSALQDCALAVAVREP
                     RFWRALPEFIAKGVSPEA"
     gene            260..895
                     /locus_tag="VNU48_09190"
     CDS             260..895
                     /locus_tag="VNU48_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11489.1"
                     /translation="MLQSLRWLAEVDARAIRHDVMRMLSCPNARRAGLDAALGTWLAQ
                     GSLDVGSVAFGPLGALDPACLNTPLARALEAQGHTAREALGGFDGVRPRGFELALRRG
                     DLAALEWWLARAPELANQVPPAQGNQLAWLPLARVLRPGFLDDPASQPEVVELLLARG
                     ADPWRTLPYDRGSTVVQYARALRSPLLGLLDPSGVPTVLAVSTATAAVAGQ"
     gene            complement(909..2696)
                     /locus_tag="VNU48_09195"
     CDS             complement(909..2696)
                     /locus_tag="VNU48_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HWI11490.1"
                     /translation="MTTAPADTAPKPSSNFLRQIVERDLADGRYAQRRFAGTPGDAAH
                     HAAGPVDTAKIRTRFPPEPNGYLHIGHAKSITLNFGLAAEYGGVCHLRFDDTNPEKEE
                     QEYVDAILDAVRWLGFDWHANGTSHLYYASNYFDFMYRAAEALIVAGLAYVDEQSADE
                     MRHHRGDFGHAGTDSPFRTRTPAENLARFRAMRAGELADGAAVLRAKLDMASPNINLR
                     DPALYRIKHAEHHNTGNAWCIYPMYAYAHPIEDALEGITHSICTLEFEDQRPWYDWLL
                     DTLCTLGLLTQPRPHQYEFARLNVSYVITSKRKLKQLVDEGIVDGWDDPRMPTIVGLR
                     RRGYTPEAIRALCERAGTSKAGGWTDYASLDAALREDLEGKAARAMAVVDPVRLRLTN
                     WAEVFGSADFREPCHAPANPHRPELAPREFSLGSEVWIERDDFAEVPAKGFFRLFPGN
                     KVRLKYGVVIECSGCEKDASGAITAVTATVVPDTKSGTPGADAVKVKGTITWVGVHDA
                     IAAELRLYDRLFTEAQPDAGGKDFKAALNPHSKTVANGFVEPSLATARPDDKFQFERH
                     GYFVADRVDHTAGTPVFNRIAGLKDSWGK"
     gene            2886..3275
                     /locus_tag="VNU48_09200"
     CDS             2886..3275
                     /locus_tag="VNU48_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11491.1"
                     /translation="MIKTAFLALITSAATLGAGSAHAGGVAWSIGINTPIVGTVISNA
                     PVYARGYAPVYAPEPVYESAPVYAPVPVYRAEPSVAYYPAPIYYRPAPVVYPRYYPGW
                     RHGGWRDHDHNRYRDHDRDHDRRDGHR"
     gene            3451..3915
                     /locus_tag="VNU48_09205"
     CDS             3451..3915
                     /locus_tag="VNU48_09205"
                     /inference="COORDINATES: protein motif:HMM:NF017269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HWI11492.1"
                     /translation="MNTRRISTAAALASLALLSACVVAPPRERVVYAPQPVVRQVPVY
                     AEYGRVENIGYVQVSQRPSGAGAVLGAVIGGVIGNRFGEGSGRALATGAGVVGGAVVG
                     NAIEGRQRRDDEVYRVQVRFDNGGVRDFDFQRIDDLRVGDRVKFEGGQLHRL"
     gene            complement(3934..>4335)
                     /locus_tag="VNU48_09210"
     CDS             complement(3934..>4335)
                     /locus_tag="VNU48_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HWI11493.1"
                     /translation="ALDATLAQLRELLAAPGLAATRTAPPLLSVIQASVGAAIHMVPV
                     DEVLYFEAADKYVRVVTAAREYLIRTSLRELLPQLDAQRFWQIHRGTVVRADAIATAL
                     RDESGKVHLSLRGHADKLIASRLYAQLFKAM"
BASE COUNT          551 a         1491 c         1589 g          704 t
ORIGIN      
        1 tgccagcctg gacagcgtcc agcgcgccct caacgagcgc tgcggtgcct tcgtgccggg
       61 cagcctgcgc gtcgcggatc tgcggcactc ggcactgcag gattgcgcgc tcgccgtcgc
      121 ggtgcgcgag ccgcgcttct ggcgggcgct gcccgagttc atcgcgaagg gcgtgtcgcc
      181 cgaggcctga gcgcgctcgc cgctggtcga gctcgcgcag gcgcaggact gtcccgattt
      241 tcagactgcc gcgccggcgg tgctgcagtc gttgcgctgg ctcgccgagg tcgatgcgcg
      301 cgcgatccgg cacgacgtga tgcgcatgct cagctgcccg aacgcgcgcc gcgccggcct
      361 cgacgccgcg ctcggcacct ggctcgcgca gggcagcctg gacgtcggca gcgtcgcgtt
      421 cgggccgctc ggcgcgctcg acccggcctg cctgaacacg ccgctcgcgc gcgcgctgga
      481 ggcgcagggc cacaccgcac gcgaagcgct cggtggcttc gacggcgtgc ggccgcgcgg
      541 cttcgaactc gcgctgcgcc gcggcgacct ggccgcgctc gaatggtggc tcgcgcgtgc
      601 acccgaactc gcgaaccagg tgccgccggc tcagggcaat cagctggcgt ggctgccgct
      661 ggcgcgcgtg ttgcggcccg gcttcctcga cgacccggcg agccagccgg aggtcgtcga
      721 actcctgctg gcccgcggtg ccgacccgtg gcgcacgctg ccctacgacc gcggcagcac
      781 cgtcgtgcag tacgcgcgcg cattgcgcag cccgctgctc ggtctgctcg acccgtcggg
      841 cgtgccgacc gtgctggccg tgagcacggc gacggccgcg gtggccggac agtagccggc
      901 ccgtcgcgct acttgcccca cgaatccttc aggccggcga tccggttgaa caccggcgtg
      961 ccggccgtgt gatcgacgcg gtcggcgacg aagtagccgt ggcgctcgaa ctggaacttg
     1021 tcgtcgggcc gcgcggtggc gagcgagggc tcgacgaagc cgttcgcgac ggtcttgctg
     1081 tgggggttca acgcggcctt gaaatccttg ccgccggcat cgggttgcgc ttcggtgaac
     1141 aggcggtcgt acaggcgcag ctcggcggcg atcgcgtcgt gcacgccgac ccaggtgatc
     1201 gtgcccttca ccttgacggc gtcggcgccg ggcgtgccgc tcttggtgtc gggcacgaca
     1261 gtggcggtga ccgcggtgat cgcgccgctc gcgtccttct cgcagccgct gcactcgatc
     1321 acgacgccgt acttgagccg caccttgttg ccggggaaca ggcggaagaa gcctttcgcc
     1381 ggcacctcgg cgaagtcgtc gcgctcgatc cagacctcgc tgccgaggct gaattcgcgc
     1441 ggcgcgagct cggggcggtg cgggttcgcc ggggcatggc agggctcgcg gaagtccgcg
     1501 ctgccgaaca cctcggccca gttcgtcagc ctgagccgga ccgggtcgac caccgccatc
     1561 gcgcgcgcgg ccttgccttc gaggtcttcg cgcagcgcgg cgtcgaggct cgcgtaatcg
     1621 gtccagccgc cggccttgct ggtgccggcg cgctcgcaca gcgctcggat cgcctcgggc
     1681 gtgtagccgc gccggcgcag cccgacgatc gtcggcatgc gcgggtcgtc ccagccgtcg
     1741 acgatgcctt cgtcgacgag ctgtttcagc ttgcgcttgc tcgtgatcac gtagctcacg
     1801 ttcaggcgcg cgaactcgta ctggtgcggg cgcggctgcg tcagcaggcc cagcgtgcac
     1861 agcgtgtcga gcagccagtc gtaccacggc cgctggtcct cgaactcgag cgtgcagatg
     1921 ctgtgcgtga tgccttcgag cgcgtcctcg atcgggtgcg cgtaggcgta catcgggtag
     1981 atgcaccagg cgttgccggt gttgtggtgt tcggcgtgct tgatgcggta cagcgccggg
     2041 tcgcgcaggt tgatgttcgg gctggccatg tcgagcttgg cgcgcagcac cgcggcgccg
     2101 tcggccagct cgcccgcgcg catcgcgcga aagcgcgcca ggttctcggc cggcgtgcgc
     2161 gtgcggaacg ggctgtcggt gccggcatgg ccgaagtcgc cgcggtggtg acgcatctcg
     2221 tcggcgctct gctcgtccac gtacgcgagg ccggcgacga tcaacgcctc ggcggcgcga
     2281 tacatgaagt cgaaatagtt gctggcgtag tagaggtggc ttgttccgtt cgcgtgccag
     2341 tcgaagccga gccacctcac cgcatcgagg atcgcgtcga cgtactcctg ttcttccttc
     2401 tcggggttgg tgtcgtcgaa gcgcaggtgg cacacgccgc cgtactcggc ggcgaggccg
     2461 aagttcagcg tgatgctctt cgcgtgaccg atgtgcaggt agccgttcgg ctcgggcggg
     2521 aagcgcgtgc ggatcttcgc cgtgtcgacc gggccggccg catggtgcgc cgcatcaccc
     2581 ggcgtgccgg cgaagcggcg ctgcgcgtag cggccgtcgg cgaggtcgcg ctcgacgatc
     2641 tggcgcagga agttgctgct gggcttgggg gcggtgtcgg cgggtgcggt ggtcatgggg
     2701 ccaatggctg gcagcgtgcg ggagcgcgca gggtgggccg attctatcgg cggccctggg
     2761 cgtcgcaggg tcgcggcggc aaggtctctt tacctgcgga tacgcggcgc gggtcaacgt
     2821 gccacgggcc gccgcgcgtt gtaggcacac gcaggccgtt tcctggcctg acgaaggaga
     2881 gcaagatgat caagaccgcc tttctcgccc tgatcaccag cgccgcaacg ctgggggccg
     2941 gcagcgcgca cgccggtggc gtggcgtggt ccatcggcat caacacgccg atcgtcggca
     3001 ccgtgatttc caacgcaccg gtctacgcac gtggctacgc gcccgtgtat gcgcccgagc
     3061 cggtgtacga atcggcgccg gtctacgcgc cggtgccggt ctaccgcgcc gaaccgagcg
     3121 tggcctacta cccggcgccg atctactacc gcccggcgcc ggtcgtgtac ccgcgctact
     3181 acccgggctg gcgccatggc gggtggcgtg accatgacca caatcgctac cgcgatcacg
     3241 atcgcgatca cgatcgccgc gacggccacc gctgaatcgt tcccgatcac gcgcgtgggt
     3301 caacgactac acgctgtcgg cgctcgcgcc gacctgtgga cgttggccgc gccgacggca
     3361 gcgctgcgtg tttcgatgaa cattacggag tgaggccgga acgctcctgg gctgttgcgg
     3421 ttccaattcc gatctgaagg aggttcgccc atgaataccc gccgcatttc gaccgctgcc
     3481 gcgctcgcgt cgctcgccct gttgtccgcc tgcgtcgtgg cgccgccgcg tgaacgggtc
     3541 gtgtacgcgc cgcagccggt ggtccggcag gtgccggtgt acgccgagta cggccgggtc
     3601 gagaacatcg gctacgtgca ggtctcgcag cgcccgagcg gcgccggtgc ggtgctcggc
     3661 gccgtgatcg gcggcgtgat cggcaaccgc ttcggcgaag gctccggccg cgcgctggcg
     3721 accggcgccg gcgtggtcgg cggcgcggtg gtcggcaacg ccatcgaagg gcgccagcgc
     3781 cgcgacgacg aggtctaccg cgtgcaggtg cgcttcgaca acggcggtgt gcgcgacttc
     3841 gacttccagc gcatcgacga cctgcgggtc ggcgaccgcg tcaagttcga aggcgggcag
     3901 ctgcatcgcc tttgatcgcg cgctggccgc cggtcacatc gccttgaaca gctgcgcgta
     3961 gaggcggctg gcgatgagct tgtcggcatg gccgcgcagg ctcagatgga cctttcccga
     4021 ctcgtcgcgc agcgcggtgg cgatcgcgtc cgcgcgcacc acggtgccgc ggtggatctg
     4081 ccagaagcgc tgcgcgtcga gttgcggcag cagctcgcgc agcgaggtgc ggatcaggta
     4141 ctcgcgcgcc gcggtgacga cgcgcacgta cttgtcggcg gcctcgaaat acagcacctc
     4201 gtcgaccggc accatgtgga tcgccgcgcc gacactcgcc tggatcacgc tcagcaacgg
     4261 cggcgcggtg cgcgtcgccg ccaggccggg tgcggcgagc agctcgcgca gctgggccag
     4321 cgtcgcgtcc agcgc
//
LOCUS       DATMJB010000429         3091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_420150, whole genome shotgun sequence.
ACCESSION   DATMJB010000429 DATMJB010000000
VERSION     DATMJB010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420150"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1249)
                     /locus_tag="VNU48_09215"
     CDS             complement(200..1249)
                     /locus_tag="VNU48_09215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWI11494.1"
                     /translation="MNETTPSTPQRRSDSARALARVLEVDDAVLAGALLEMLDRVDVL
                     LSVKDAATGRYLHVNAAMAELFGREVATMTGAGDADLMEPAQCAAMRAADQTALAHAA
                     PTLGQQRIDRDGRKREFSVTRLPLARGDGGPARHVLSVWVEQTTAHQREQQLQQALVQ
                     LEQQQLAAEVQRRETQDQSLRDGTTGLYQRMHFDDQLRREVDLSSREHREFALVSIAL
                     DPLADAVKALGDSARTRVLEALGRLLRSNTRAMDASCRLDEDRFAVLLSGVGLATAHS
                     RMEGLRRQCATQIVVLEGRDLGFTVSMGVASFPHTAHSEEELLQASDTALREAQKRGG
                     NHVALASIRFELGAS"
     gene            1499..2452
                     /gene="ybiB"
                     /locus_tag="VNU48_09220"
     CDS             1499..2452
                     /gene="ybiB"
                     /locus_tag="VNU48_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HWI11495.1"
                     /translation="MAIASYIKEIGRGKDGARSLTETQAHDLMSQVLDGRVTDLEIGA
                     FALAMRIKGESTAELAGFVRAAHERCIAIRPARPTILLPSYNGARKLPNLTPLLALLL
                     AQEGAQVLVHGPMLDAGRVGSAEIFRDLGLPFASNADEIENGWARREPVFIATHDLCP
                     PLASLLQVRRVVGLRNSGHTVAKVLSPCSGAASLRVVNHTHPEYATMLADFLAYTGGD
                     AVLLRGTEGEPVADPRRAPRLDVFIEGRPRADLSRAAHEGVLTELPVLPRGFDAATTA
                     VYIQAVVSGEKPAPGPLTRQVECLMGALGAIRRAAPQEQIA"
     gene            2525..>3091
                     /locus_tag="VNU48_09225"
     CDS             2525..>3091
                     /locus_tag="VNU48_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWI11496.1"
                     /translation="MKQELDKLNKLSESHGALRPGKGLVSGVIALSLAILCFLGVLAF
                     HWPQYLTTPQLRQSYNVDVMRLLLFWAMVLAGAIALYNIISGRARWLAFAAFVLIGLS
                     LALGGHKVPVDSNFPDHTPYIGLDWFILDLLGSSLIFIFIEKLFAHRKDQPVFRAEWQ
                     TDFHHFVVNHMIVGFVLLATNLLVHKLFG"
BASE COUNT          430 a         1094 c         1073 g          494 t
ORIGIN      
        1 gccggccctg accggcatgg ccacgctgcg gcgcggcaac cgcttgtcga tcacgccggt
       61 cacggccgat gagtggcgta cggtgctggc gctgctgaag aagccgggat gaccggcgcc
      121 tgcggcgcgt gttcgcggcg ctgctgttcg gcctgacggc cgcatgatcg acaaggcgcc
      181 gggcgcctga acgcgagcct cagctcgcgc ccagctcgaa gcggatgctc gccagcgcga
      241 cgtggttgcc gccgcgcttc tgggcctcgc gcaacgcggt gtcggacgcc tgcagcaact
      301 cttcctcgct gtgcgcggtg tgcgggaagc tcgccacgcc catcgacacc gtgaagccca
      361 ggtcgcgccc ttcgagcacg acgatctgcg tcgcgcattg gcgccgcagc ccttccatgc
      421 gcgagtgcgc agtcgccagc ccgacgcccg acagcagcac cgcgaaacgg tcttcgtcga
      481 gccggcagga cgcatccatc gcccgggtgt tgctgcgcag cagccgcccg agcgcctcga
      541 gcacgcgggt gcgggcgctg tcgccgagcg ccttgacggc gtcggcgagc gggtcgaggg
      601 cgatcgagac gagcgcgaac tcgcggtgct cgcgcgacga caggtcgacc tcgcgacgca
      661 gctggtcgtc gaagtgcatg cgctggtaca ggccggtggt gccgtcgcgc aggctttgat
      721 cttgggtctc gcggcgctgc acctcggcgg cgagctgttg ctgttcgagc tgcaccagcg
      781 cctgctgcag ctgctgctcg cgctggtgcg cggtggtctg ctcgacccac acgctcaaca
      841 cgtggcgcgc cgggccgccg tcgccccgcg ccagcggcaa gcgtgtgacg ctgaattcgc
      901 gcttgcgacc gtcgcggtcg atgcgctgct ggccgagcgt cggggcggcg tgcgccagcg
      961 cggtctggtc ggccgcgcgc atcgcggcgc attgggccgg ctccatcaga tcggcgtcgc
     1021 cggcaccggt catcgtcgcg acctcgcggc cgaagagttc ggccatcgcc gcgttgacgt
     1081 gcaggtagcg gccggtcgcg gcgtccttga ccgagagcag cacgtcgacc cggtcgagca
     1141 tttccagcag ggcaccggcc agcaccgcgt cgtcgacctc gagcacgcgg gccagcgcgc
     1201 gcgcgctgtc gctgcggcgt tgcggcgtgc tcggtgttgt ctcgttcatc agcgccatct
     1261 tccctgagat ccacagccat cttggtctcg ggacccgccc ctggcaagcg ctgcacgccg
     1321 cgaatcacga gtccacccct agcttgatga tagcggcacg cgtggtctgt gctagccggt
     1381 tttcgaagcc ccggatcggg gttgcctgcc ccggaccacg gcacgggcat ttgaaattca
     1441 ttgttgccgg atcgccggcg ggcctgaaaa ttgcgatccg cgctgccagg agacaagcat
     1501 ggcgatcgca agctacatca aggaaatcgg ccgcggcaag gacggcgccc gctcgttgac
     1561 cgagactcag gcgcacgacc tgatgagcca ggtgctcgac ggccgggtca ccgacctgga
     1621 gatcggtgcg ttcgcgctgg cgatgcgcat caagggcgag tcgaccgccg agctcgccgg
     1681 cttcgtgcgc gccgcgcacg agcgctgcat agccatccgg ccggcccggc cgaccatcct
     1741 gctgccctcg tacaacggcg cgcgcaagtt gcccaacctg acgccactgc tggcactgct
     1801 gctggcccag gaaggcgcgc aggtgctggt gcacggcccg atgctggacg ccggccgcgt
     1861 cggcagtgcc gagatcttcc gcgatctcgg gctgcccttc gcgagcaatg ccgacgagat
     1921 cgagaacggc tgggcgcggc gcgagccggt cttcatcgcc acgcatgacc tgtgcccgcc
     1981 gctcgcaagc ctgctccagg tgcgccgtgt cgtcgggctg cgcaactcgg gccacacggt
     2041 ggcgaaggtg ctgtccccct gcagcggcgc cgcgtcgctg cgggtcgtca accacacgca
     2101 cccggagtac gcgacgatgc tggccgattt cctggcctac acgggcggcg acgccgtgct
     2161 gctgcgcggc accgagggcg agccggtcgc cgacccgcga cgcgctccgc gcctcgacgt
     2221 gttcatcgag ggccggccgc gcgccgacct gtcgcgtgcc gcgcacgagg gcgtgctgac
     2281 cgagctgccg gtgctgccgc gcggcttcga cgcagcgacc accgcggtct acatccaggc
     2341 cgtcgtcagc ggcgagaagc cggcgccggg gccgctgacg cggcaggtcg agtgcctgat
     2401 gggcgcgctc ggcgcgatcc gccgcgccgc gccgcaggag cagatcgcct gaaccggagg
     2461 cccggaatgc gcgccgcata atgcgcggcg gttcgagcca ccggacctgg cctgggaggg
     2521 cgccatgaag caggaactcg acaagctcaa caagctgagc gaatcgcacg gcgcgctgcg
     2581 gcccggcaag ggcctggtgt cgggtgtgat cgcactctcg ctggcgatcc tgtgctttct
     2641 gggcgtgctg gcctttcact ggccgcagta cctgacgacg ccgcagctgc gtcagtcgta
     2701 caacgtcgac gtgatgcggt tgctgctgtt ctgggctatg gtgctggccg gcgcgatcgc
     2761 gctgtacaac atcatcagcg ggcgcgcgcg ctggctcgcg ttcgccgcgt tcgtactgat
     2821 cggcctgtcc ctcgcgctcg gcggccacaa ggtgcctgtg gacagcaact tccccgacca
     2881 cacgccctac atcggcctgg actggttcat cctcgacctg ctgggctcct cgctgatctt
     2941 catcttcatc gagaagctgt tcgcgcaccg caaggaccag ccggtgttcc gcgccgagtg
     3001 gcagaccgac ttccaccact tcgtcgtcaa ccacatgatc gtgggcttcg tgctgctcgc
     3061 gaccaacctg ctggtgcaca agctcttcgg c
//
LOCUS       DATMJB010000430         2469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_407191, whole genome shotgun sequence.
ACCESSION   DATMJB010000430 DATMJB010000000
VERSION     DATMJB010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407191"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..1695
                     /locus_tag="VNU48_09230"
     CDS             28..1695
                     /locus_tag="VNU48_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWI11497.1"
                     /translation="MTHADFDFDWLVIGSGFGGSVSALRLADKGYRVGVFERGRRYRD
                     EDFPKSTWQFPKFVWAPLFGLKGIFRMALFRHVFALSGAAVGGGSVVYSNTLYRAKPE
                     FFENAQWQALGRWAELLRPHYDTAERMLGVQKVPFDSPNQQLIREMARHFGTEDTFTR
                     TPCAVYFGEPGKTVSDPYFGGDGPDRTGCTRCGACMVGCRVGAKNTLVKNYLWFAGRR
                     GAQIMPEREVVDVTPLGALDGSDGYLVSTERPGAWFFRDRQTHTARGVVFAAGALGTN
                     ELLANLKHGGSLPRISDRLGELVRTNSESILAVRLPEDRKTWNDVAISCSVHINHDTH
                     IEFVTYGRNADFIGTLYSVLVGKGNNITRPLKWLGAALLHPVQLLKSLWPVGWSQHTV
                     ILLVMQALDNAISLRAKKRWWGRGYRLATAQNADKPNPTYIESANQATQWLAKHTGGI
                     AQSNLLEALGNIPTTAHLLGGAVIAADAASGVIDANLQVFGYRNLLVCDGAAMPANPG
                     VNPSLTITALAEHAMAQIPAARPAPPEPVTERPASHRAGATATLASV"
     gene            1704..>2469
                     /locus_tag="VNU48_09235"
     CDS             1704..>2469
                     /locus_tag="VNU48_09235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI11498.1"
                     /translation="MSASPIVIVGTGLAGYGLARELRKLDAEVPIVMVSRDAGDFYSK
                     PMLSNALGAKRSAASLVMKSSVAMGAELRARILERTQVTGIDLQARCLQLEGGARQPY
                     SALVLAVGADPIRLALNGNAVGDLLSVNDLDDFSVFEKALAVLDGRNSSRNVLILGAG
                     LIGCEFANDLLARGFSPTVVDLADRVLERLLLPRASQRLRTNLENAGVAFRFKTRVTS
                     VNRSADGFSVEFSDGTSMPCDLVISAIGLNPRIALAA"
BASE COUNT          426 a          815 c          807 g          421 t
ORIGIN      
        1 taaagaaaaa gagaagagga gacaaaattg acccacgctg atttcgactt cgattggctg
       61 gtgatcggtt ccggcttcgg cggcagcgtt tcggcgctgc gcctggccga taaaggctac
      121 cgcgtgggcg tattcgagcg cggccggcgc taccgcgacg aagattttcc caagtcgacc
      181 tggcagtttc ccaagttcgt gtgggcgccg ttgttcggcc tgaagggcat ctttcgaatg
      241 gcgctgtttc gccacgtctt tgccctcagc ggcgctgccg tcggtggcgg cagcgtcgtg
      301 tattccaaca ccttgtaccg agccaagccg gagtttttcg agaatgcgca gtggcaagcg
      361 ctgggccgct gggccgagct gctgcggccg cactacgaca cggcggagcg catgctcggc
      421 gtccagaagg tgcccttcga ttcgcccaac cagcagttga tccgcgagat ggcgcggcac
      481 ttcgggaccg aagacacgtt cacccgcacg ccttgcgcgg tctacttcgg cgagccgggc
      541 aagacggtga gcgaccccta cttcggtggc gatgggcctg accgcaccgg ctgtacgcgc
      601 tgcggcgcct gcatggtggg ctgccgcgtg ggcgccaaga acacactggt gaaaaactac
      661 ctgtggttcg cggggcggcg cggcgcgcag atcatgccgg aacgcgaggt cgtcgacgtc
      721 accccgctgg gcgctctcga cggcagcgat ggctacctcg tgagcaccga gcgccccggc
      781 gcctggttct tccgcgatcg gcagacccac accgcccgag gcgtggtttt cgccgccggc
      841 gcgctcggca ccaacgaact gctggccaac ttgaagcatg gcggctcgct gccccggatc
      901 agcgaccgcc tcggtgaact ggtgcgcacc aacagcgaat ccatcctcgc ggtgcgcctg
      961 cccgaggacc gcaagacctg gaacgacgtc gccatcagct gcagcgtgca catcaatcac
     1021 gacacccaca tcgagttcgt cacctatggc cgcaacgccg atttcatcgg cacgctctac
     1081 tcggtgctgg tcggcaaggg caacaacatc acgcgaccgc tcaagtggct gggcgccgcc
     1141 ctgctgcacc cggtgcaatt gttgaagtcg ctgtggcctg tcggctggag ccagcacacg
     1201 gtgatcctgc tggtgatgca ggcactggac aacgcgatct cgctgcgcgc caagaagcgc
     1261 tggtggggcc gcggctaccg cctcgccacc gcacagaatg ccgacaagcc gaacccgacc
     1321 tacatcgaaa gcgccaatca ggcgacccag tggctggcca agcacacggg cggcatcgcg
     1381 caaagcaact tgctcgaggc gctgggcaat attccgacca cggcccacct gctcggcggg
     1441 gccgtcatcg ctgccgatgc cgccagcggc gtgatcgatg ccaatctgca ggtctttggc
     1501 taccggaacc tgctggtgtg cgacggcgcc gccatgccgg caaatcccgg ggtcaatccg
     1561 tcgctgacga tcacggcact cgccgagcac gccatggcgc agattccggc ggcccgtccg
     1621 gcgccaccgg agcccgtgac cgagcgcccg gcgtcacatc gggccggggc gaccgcgacg
     1681 ctcgcgagcg tctgaagcac atcgtgagcg cttcacccat cgtcatcgtc ggaaccgggc
     1741 tggccggata cggcctcgcc agggaactgc gaaagctcga tgccgaagtt cccatcgtga
     1801 tggtcagccg ggacgcgggc gatttttatt cgaagccgat gctgtccaat gcgctggggg
     1861 cgaagcgatc tgccgcctcc ctggtcatga agtcatcggt ggccatgggg gcggaactgc
     1921 gtgcgcggat tctcgagcgc acccaggtca ccggcatcga tctgcaggcc cgttgtctgc
     1981 aactggaggg cggagcacgg cagccctaca gcgcgctggt gctggcggtg ggcgcggatc
     2041 cgatccgcct ggcgctgaac ggaaacgccg tgggcgacct gctttccgtg aacgacctgg
     2101 acgacttttc ggtgttcgag aaagcactgg ccgtgctcga cggccgcaac agctcgcgga
     2161 acgtgctcat cctgggtgcc ggcctgatcg gttgcgaatt cgccaacgat ctgttggccc
     2221 gcgggttcag cccgactgtc gtcgacctgg ccgatcgggt gctggagcgt ctgctgctgc
     2281 ctcgagccag ccagcggctg cgaacaaacc tcgagaacgc cggcgtggca tttcggttca
     2341 agacgcgagt gacctcggtc aaccgttcgg ccgacgggtt cagcgtggaa ttttcagacg
     2401 gcacgtcaat gccgtgcgat ctcgtcatct ccgccatcgg actgaatccg cggatcgcgc
     2461 tcgcagcgc
//
LOCUS       DATMJB010000431         4307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_120849, whole genome shotgun sequence.
ACCESSION   DATMJB010000431 DATMJB010000000
VERSION     DATMJB010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120849"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VNU48_09240"
     CDS             complement(<1..493)
                     /locus_tag="VNU48_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HWI11499.1"
                     /translation="MLYQFYETQRALLSPFAEFASATSKLYNHPLSPFAHTPGAQRVS
                     AGLDLMHRLSKDYEKPEFGITSAKVDGVDVAVQEQVALTKPFCRLLRFKRFTDNVAVL
                     TRLKGQPTVLVVAPLSGHHSTLLRETVRELLKDHKVFITDWIDARMVPKEAGPFHLDD
                     YVTY"
     gene            complement(649..1863)
                     /locus_tag="VNU48_09245"
     CDS             complement(649..1863)
                     /locus_tag="VNU48_09245"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HWI11500.1"
                     /translation="MSSLFAAVEMAPRDPILGLNEQFNADPNPAKVNLGVGVYFDDNG
                     KLPLLRCVQEAEKMMMEAPKARGYLPIDGIPAYDKAVQGLVFGADSAALKAGRIATVQ
                     ALGGTGGLKVGADLLKRMNPGATVLISDPSWENHRALFEGAGFRVEQYPYYDAPTPDK
                     SGGIDFDAMLGALDKAAPGTIVVLHACCHNPTGYDITPAQWAQVVDTVKARALVPFLD
                     MAYQGFGEGIQQDGAVVGQFIDAGLAFFVATSFSKSFSLYGERVGALSVVCDSKDEAE
                     RVLSQLKRVIRSNYSNPPTHGAQVVALVLTTPALRALWEEELAGMRLRIKEMRVALQS
                     RLQAAGLKQDVGFITRQTGMFSYSGLNKAQMQRLRSEFGIYGVDSGRICVAALNTKNI
                     DATVAAIVKVSA"
     gene            1979..4099
                     /gene="uvrB"
                     /locus_tag="VNU48_09250"
     CDS             1979..4099
                     /gene="uvrB"
                     /locus_tag="VNU48_09250"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HWI11501.1"
                     /translation="MAELSEVIDPAAAPAVPEGEFISYPDSPFQLFQPYPPAGDQPTA
                     IAGLVEGLRDGLSFQTLLGVTGSGKTFTMANVIAQMGRPAIVFAPNKTLAAQLYSEFR
                     EFFPKNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSSINEHIEQMRLSATKSLLERRDV
                     VIVASVSAIYGIGNPSDYHQMVMTLRVRDKLGQRDVIAQLIRMQYQRNETDFSRGTFR
                     VRGDTIDVFPAEHSELAIRIELFDDEVESLQLFDPLTGRIRQKIPRFTVYPSSHYVTP
                     RERVVVAIDTIKAELRERLDELVKMGKLVEAQRLEQRTRFDLEMLQEVGHCKGIENYT
                     RHLSGAKPGDPPPTLVDYLPADALMFLDESHVLIGQFGGMYNGDRARKTTLVEYGFRL
                     PSALDNRPLKFEEFERKMRQVMYVSATPAAYEQQTSGQTVEQLVRPTGLIDPPVDVRP
                     AINQVDDVLQEIRDRVEINERVLITTLTKRMAEQLTDYLADNGVKVRYLHSDVDTVER
                     VEILRDLRLGAFDVLVGINLLREGLDIPEVSLVAILDADKEGFLRSERSLIQTIGRAA
                     RNLNGRAILYADKVTESMRKAIDETDRRRAKQIAHNLAEGITPRSVVKRVRDLIDGVY
                     SEKGAKDDLKSAVESAAIEALSEKDLGKRIKQLEKQMLDHARNLEFEKAARVRDQLAH
                     LREQAFGAPGTDNIVPLNAGRGSQ"
BASE COUNT          748 a         1429 c         1434 g          696 t
ORIGIN      
        1 cataggtcac gtagtcgtcg aggtggaacg ggccggcttc cttcggcacc atgcgtgcgt
       61 cgatccagtc ggtgatgaag accttgtggt ccttcagcaa ctcgcgcacg gtctcgcgca
      121 gcagcgtcga atggtggccc gacagcggcg ccaccacgag cacggtcggc tggcccttca
      181 ggcgggtcag cacggcgacg ttgtcggtga atcgcttgaa gcgcagcagc cggcagaacg
      241 gcttggtcag ggcgacctgc tcctgcaccg cgacgtccac gccatcgacc tttgccgagg
      301 tgatgccgaa ctcgggcttc tcgtagtcct tcgacaaccg gtgcatcaga tccagaccgg
      361 ccgacacgcg ctgcgcaccg ggggtgtgcg cgaacggcga gagcgggtgg ttgtagagct
      421 tggaggtcgc gctcgcgaac tcggcgaacg ggctcagcaa tgcacgttgg gtttcataga
      481 actgatacag catctcaaca cctggtgggt gaatcggcgg ggatgtgcgg cacgatgttt
      541 gtgcaacgca acattgtaag cgcgcgtgca cgatcttgca gcagggccat ccggggtgaa
      601 acccggatca gcgcccggtt tgggcgcttg cgcgccggcg acgtgcgttc aggccgagac
      661 cttgacgatc gcggcaacgg tggcgtcgat gttcttcgtg ttcagcgcgg cgacgcagat
      721 ccggccggag tcgacgccgt agatgccgaa ctcgctgcgc agccgctgca tctgcgcctt
      781 gttcagcccc gaatagctga acatgccggt ctgccgcgtg atgaagccca cgtcctgctt
      841 cagccccgcc gcctgcagcc gggactgcag cgcgacgcgc atctccttga tgcgcaggcg
      901 catgccggcg agttcttctt cccacagcgc gcgcagcgcc ggcgtggtca gcaccagcgc
      961 gacgacctgc gcgccgtgcg tcggcgggtt cgagtagttg ctgcggatca cgcgcttgag
     1021 ctggctcagc acgcgctcgg cctcgtcctt cgagtcgcag acgacgctga gggcgccgac
     1081 gcgctcgccg tacagcgaga agctcttcga gaagctggtc gcgacgaaga aggccaggcc
     1141 ggcgtcgatg aactggccga cgaccgcgcc gtcctgctgg atgccttcgc cgaagccctg
     1201 gtaggccatg tcgagaaatg gcaccagcgc gcgcgccttg acggtgtcca ccacctgcgc
     1261 ccactgagcc ggggtgatgt cgtagccggt cgggttgtgg cagcaggcat gcagcacgac
     1321 gatcgtgccc ggcgcggcct tgtcgagcgc acccagcatc gcgtcgaaat cgatgccgcc
     1381 gctcttgtcg ggcgtgggcg cgtcgtagta ggggtactgc tcgacccgga agcccgcacc
     1441 ctcgaacaac gcgcggtggt tctcccagct cgggtcgctg atcagcacgg tcgcacccgg
     1501 gttcatgcgc ttgagcagat cggcgccgac cttcagcccg ccggtgccac cgagcgcctg
     1561 caccgtcgcg atgcggccgg ccttcagcgc cgcgctgtcg gcgccgaaca ccaggccctg
     1621 caccgccttg tcgtaggccg ggatgccgtc gatcggcaga tagcctcttg ccttcggcgc
     1681 ttccatcatc atcttctcgg cttcctgcac gcaccgcagc agcggcagct tgccgttgtc
     1741 gtcgaagtag acgccgacgc cgagattgac cttggccgga ttcggatccg cgttgaactg
     1801 ttcgttcagg cccaggatcg ggtcgcgggg cgccatttcg acggcggcga agagcgaaga
     1861 catggtcggt ccttgtggtc ggtggatgag catcggcgtt tccgctacgc tgcgcggttg
     1921 cccggcaagc catgcgacgg cgtgtttgcc gagagataac ccggagattt tatctgtcat
     1981 ggccgagctg tccgaagtga tcgatcccgc cgccgcgccg gcggtgcccg aaggcgagtt
     2041 catcagctac cccgactcgc cgttccagct gttccagccc tatccgccgg cgggcgacca
     2101 gccgaccgcg atcgccgggc tggtcgaggg tctgcgcgac ggcctgagct tccagaccct
     2161 gctgggcgtg accggctcgg gcaagacctt cacgatggcc aacgtgatcg cgcagatggg
     2221 ccgcccggcg atcgtgttcg cccccaacaa gacgctggcc gcgcagctgt acagcgagtt
     2281 ccgcgagttc ttcccgaaga acgcggtcga gtacttcgtc agctactacg actactacca
     2341 gcccgaggcc tacgtgccgc agcgcgacct gttcatcgag aaggacagct cgatcaacga
     2401 gcacatcgag cagatgcgcc tgagcgcgac gaaaagcctg ctggagcgtc gcgacgtcgt
     2461 gatcgtggcc agcgtctcgg cgatctacgg catcggcaac ccgagcgact atcaccagat
     2521 ggtgatgacg ctgcgggtca gggacaagct cggccagcgc gacgtgatcg cgcagctgat
     2581 ccggatgcag taccagcgca acgagaccga tttctcacgc ggcaccttcc gcgtgcgcgg
     2641 cgacacgatc gacgtgtttc cggccgagca ctccgagctg gcgatccgca tcgagctgtt
     2701 cgacgacgag gtcgagtcgc tgcagctgtt cgacccgctg accggccgca tccgccagaa
     2761 gatcccgcgc ttcacggtct acccgagcag ccactacgtg acgccgcgcg agcgcgtcgt
     2821 cgtcgcgatc gacacgatca aggccgagtt gcgcgaacgc ctcgacgagc tcgtgaagat
     2881 gggcaagctg gtcgaggcgc agcggctcga gcagcgcacc cgcttcgatc tcgagatgct
     2941 gcaggaggtc ggccactgca agggcatcga gaactacacg cggcacctgt cgggcgcgaa
     3001 acccggggac ccgccgccga ccttggtcga ctacctgccc gccgacgcgc tgatgttcct
     3061 cgacgaaagc cacgtgctga tcggccagtt cggcggcatg tacaacggcg accgggcgcg
     3121 caagaccacc ttggtcgagt acggcttccg cctgccgagc gcgctcgaca accgcccgct
     3181 gaagttcgag gagttcgaac gcaagatgcg ccaggtgatg tatgtctcgg ccacgccggc
     3241 ggcctacgag cagcagacgt ccggccagac cgtcgagcag ctggtgcgcc cgaccggtct
     3301 gatcgatccg ccggtggatg tgcgccccgc gatcaaccag gtcgacgacg tgctgcagga
     3361 gatccgcgac cgggtcgaga tcaacgagcg cgtgctgatc acgacgctga ccaagcgcat
     3421 ggccgagcag ctcaccgact acctcgccga caacggcgtc aaggtgcgct acctgcacag
     3481 cgacgtcgac acggtcgagc gcgtcgagat cctgcgcgac ctgcggctcg gtgccttcga
     3541 cgtgctggtg ggcatcaacc tgctgcgcga agggctggac atccccgagg tctcgctggt
     3601 cgcgatcctc gatgccgaca aggagggctt tctgcgctcc gagcgcagcc tgatccagac
     3661 catcggccgg gcggcgcgca atctcaacgg ccgcgcgatc ctgtacgccg acaaggtcac
     3721 cgagtcgatg cgcaaggcga tcgacgagac cgatcggcgt cgcgcgaagc agatcgccca
     3781 caacctggcc gagggcatca cgccgcgcag cgtggtcaag cgcgtgcgcg acctgatcga
     3841 tggcgtctac agcgaaaagg gcgcgaagga cgacctgaag tccgccgtcg agtccgcggc
     3901 gatcgaggcg ctcagcgaga aggacctggg caagcggatc aagcagctcg agaagcagat
     3961 gctcgaccac gcccgcaacc tcgagttcga gaaggcggcg cgggtacgtg accagctggc
     4021 gcacctgcgc gagcaggcct tcggcgcgcc gggcaccgac aacatcgtgc cgctgaacgc
     4081 ggggcgcggc agccagtgac cgacacgcgg cgcggcgcgc gggcgggcac catttccctg
     4141 tcggccccag ggaacttccc ggcccaatct cgaccgaatt tgtcggcttc ggctatactt
     4201 gacggaacta gtcggaatag agcgggttca ctcgcagatc cggcgccgtc gggttgccga
     4261 ggctgccatt ccgtagctga acagccgacc gaggctttag agatcgg
//
LOCUS       DATMJB010000432         3126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_114060, whole genome shotgun sequence.
ACCESSION   DATMJB010000432 DATMJB010000000
VERSION     DATMJB010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114060"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VNU48_09255"
     CDS             <1..519
                     /locus_tag="VNU48_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI11502.1"
                     /translation="GLPCIVLRTSRFFPEDDDRAQTRADYFDANAKVNEFLHRRVDIE
                     DVVDAHLLAIERAPAIGFGRYIISATSPFRHDELAALRDDAPAVAAAHVPEYEAEYRR
                     RGWTMFPGIDRVYVNARARQELGWRPRHDFSSVVARLCAGGDHRSALAQAIGAKGYHE
                     TTFADRPYPVAP"
     gene            701..835
                     /locus_tag="VNU48_09260"
     CDS             701..835
                     /locus_tag="VNU48_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11503.1"
                     /translation="MSRSRSVAAALKFVRAAFEAALVFRAETVKANDSAAALKKSGAT
                     "
     gene            complement(813..1406)
                     /locus_tag="VNU48_09265"
     CDS             complement(813..1406)
                     /locus_tag="VNU48_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HWI11504.1"
                     /translation="MHKPFSPACERNQDAILAVLREHFADRTQVLEIGSGTGQHAVHF
                     AAALPQLIWQTSDVAANLSGIRAWLAGARLPNTPAPLAFDINAAPPDATFDAIFSANT
                     LHIMAWPEVQNLFALLPALMRPDALLTIYGPFNHGGTFTSASNAAFDASLRADQPQRG
                     IRDFEAVDALAGAAGLVLVEDRAMPANNRCITWRRSS"
     gene            1467..2069
                     /locus_tag="VNU48_09270"
     CDS             1467..2069
                     /locus_tag="VNU48_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWI11505.1"
                     /translation="MTTRPLIALDCDGVLLDFHLGYASAWARAFGQAPAERDPLAYWP
                     MDRWEVERLDAERRVLFRAAFDEHFWSTMPPLPGALEACHRLHDAGFDLVCVSALELE
                     FEAARRRNLRNLGFPIERVIATGNAEGKRSPKADAIERLTPIAFVDDYLPYLRGLPRH
                     LHTALVLRAPHGSPNVGADLALAKSVHDDLAGFVGHWLAR"
     gene            complement(2080..2298)
                     /locus_tag="VNU48_09275"
     CDS             complement(2080..2298)
                     /locus_tag="VNU48_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HWI11506.1"
                     /translation="MSAGTTGARVIRCPGCGGPSRYAPDNAFRPFCSARCKNNDFGAW
                     ASEAFAVPADPDPDRPDPNADSGDTLPH"
     gene            complement(2295..3050)
                     /gene="zapD"
                     /locus_tag="VNU48_09280"
     CDS             complement(2295..3050)
                     /gene="zapD"
                     /locus_tag="VNU48_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HWI11507.1"
                     /translation="MILYEYPFNESIRTMLRLEHLFDRLGQLIARDTPVDHHYALATL
                     FEILDVSSRADLKSDLLKELDKHRQQFNSYRGNPSISEAALDEAIAKIDHAFNGLNEV
                     QGKAGAALNSNEWLMSIRSRISIPGGTCEFDLPAYYAWQKFEPQRRRADLLQWIGSLM
                     PLAEALQVLLGLLRDSGVPQKVVAPAGQYQQSLPQGRVYQLMRVRLDVPEDIIPEISG
                     HRLMVSVRLMRQDAEGRLKPSQQDANFELTLCA"
BASE COUNT          471 a         1050 c         1106 g          499 t
ORIGIN      
        1 gggctgccct gcatcgtgct gcggacctcg cgcttcttcc cggaggacga cgaccgggcg
       61 cagacccgcg cggactactt cgacgccaat gccaaggtca acgaattcct gcaccggcgc
      121 gtcgacatcg aagatgtggt cgacgcgcat ctgctggcca tcgagcgcgc gccggcgatc
      181 gggttcggtc gctacatcat cagcgcgacc tcgccattcc ggcatgacga actggcggcg
      241 ctgcgcgacg atgcgcctgc ggtggccgcc gcgcatgtgc cggaatacga ggccgagtac
      301 cggcgccgcg gctggacgat gttcccgggc atcgatcgcg tctacgtcaa cgccagggcg
      361 cgccaggaac tgggctggcg accgcgccac gacttcagct ccgtcgttgc gcggctgtgc
      421 gccggcggcg atcaccgcag tgcgctggcg caggcgatcg gcgcgaaggg ctaccacgag
      481 accacgttcg ccgaccgtcc ctacccggtc gcgccctaga cgacgattca cctcagcggc
      541 ggcatggacg cttcgttcct gcacctcgaa acgcccgaga tgccgatgca cgtcggcagc
      601 ctgcagatcg ccgacctaac aagatgcacc accgaggtcg acggcaagcg ccatctcagc
      661 ctgccggaag ggttccagct cggcccgaag acgccgctga atgtcgcgat cacgctcggt
      721 cgcggccgcg ctgaagttcg tgcgcgcggc gttcgaggcg gcacttgtgt tccgcgccga
      781 gaccgtgaag gcgaacgact ccgccgcggc gctcaagaag agcggcgcca cgtgatgcag
      841 cggttgttgg ccggcatcgc gcggtcttcg acgagcacca ggccggcggc gccggccagc
      901 gcatccacgg cctcgaagtc gcggatgccg cgctgcggct gatcggcgcg cagcgacgcg
      961 tcgaaggcgg cgttgctggc gctggtaaag gtgccgccgt ggttgaacgg cccgtagatc
     1021 gtcagcagcg catcaggccg cattagcgcg ggcaacagcg cgaacaggtt ctgcacttcg
     1081 ggccaggcca tgatgtgcaa ggtgttcgcg ctgaagatcg catcgaaggt ggcgtcgggc
     1141 ggcgcggcgt tgatgtcgaa tgcgagcggc gccggcgtgt tgggcagacg ggccccggcg
     1201 agccacgcgc gaatgccgga caggttcgcc gcgacgtccg aggtctgcca gatcagctgc
     1261 ggcaacgccg ccgcgaaatg caccgcgtgc tggccggtgc cgctgccgat ctcgagcacc
     1321 tgcgtgcgat cggcgaagtg ctcgcgcagc accgcgagga tcgcgtcttg gttgcgctcg
     1381 caggcgggcg agaagggctt gtgcatcgcg cattgtgcgt ccggcaggcg acagcgcgcg
     1441 gcgtgcgggg gcgctagagt cacgtcatga ccactcgccc gctgattgcc ctcgattgcg
     1501 acggtgtgct gctcgatttc cacctcggct atgcgtcggc ctgggcgcgc gcgttcggcc
     1561 aggcgccggc cgagcgcgac ccgctcgcct actggccgat ggaccgctgg gaggtcgagc
     1621 gcctcgacgc cgagcgacgg gttctgtttc gcgccgcgtt cgacgaacac ttctggagca
     1681 cgatgccgcc gctccccggc gcgctcgagg cctgccatcg gctgcacgac gccggcttcg
     1741 atctcgtctg tgtctcggcg ctcgagctcg aattcgaggc cgcgcggcgg cgcaacctgc
     1801 gcaacctggg cttcccgatc gagcgcgtga tcgccacggg caacgccgag ggcaagcgca
     1861 gccccaaggc cgatgcgatc gagaggctga ccccgatcgc gtttgtcgac gactacctcc
     1921 cgtacctgcg cgggctgccc cgccatctgc ataccgcgct ggtgctgcgc gcgccgcacg
     1981 gcagcccgaa tgtcggcgct gatctggcgc tggcgaagtc ggtgcacgac gacctcgcgg
     2041 gtttcgtcgg gcactggctc gcgcgctgaa gcgaccggct cagtgcggca gcgtatcgcc
     2101 ggaatcggcg ttcggatcgg gccggtcggg gtccggatcg gcgggcaccg caaaggcttc
     2161 gctggcccag gcgccgaagt cgttgttctt gcagcgtgcg ctgcagaacg ggcggaaggc
     2221 gttgtcgggc gcgtagcggc tcgggccgcc gcagcccggg cagcggatca cccgcgcacc
     2281 ggtcgtgccc gcgctcatgc gcaaagcgtc aactcgaagt tggcgtcctg ctggctgggc
     2341 ttgaggcggc cttcggcgtc ctgccgcatg agtcgcaccg acaccatcag ccggtggccg
     2401 ctgatctcgg gaatgatgtc ttcgggcacg tccagccgca cccgcatcag ctgatagacg
     2461 cggccctgcg gcaggctctg ctggtactgg ccggcgggcg cgacgacctt ctgcggcacg
     2521 ccggagtcgc gcaacaggcc cagcagcacc tgcagcgcct cggccagcgg catcagtgag
     2581 ccgatccact gcagcaggtc ggcgcggcgc cgctgcggct cgaacttctg ccaggcgtaa
     2641 taggccggca ggtcgaactc gcaggtgccg ccagggatgc tgatgcggct gcggatgctc
     2701 atcagccact cgttgctgtt cagtgccgcg ccggccttgc cctgcacctc gttcaggccg
     2761 ttgaacgcgt gatcgatctt cgcgatcgcc tcgtcgagcg cggcttccga gatcgacggg
     2821 ttgccgcggt agctgttgaa ctgttgccgg tgcttgtcga gctccttcaa caggtcggat
     2881 ttcagatcgg cgcgcgagga gacgtcgagg atctcgaaca gggttgcgag cgcgtagtgg
     2941 tggtcgaccg gtgtgtcgcg cgcgatcagc tggccgagcc gatcgaacag atgctcgagg
     3001 cgcagcatcg tgcgaatgct ttcgttgaac gggtattcgt aaaggatcac ggtaggaagc
     3061 cctgagccgg cgaagcgaac cagcgagcgc cgattgttcc acagcgagcc gctgcgccgc
     3121 ggcggc
//
LOCUS       DATMJB010000433         2420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_347788, whole genome shotgun sequence.
ACCESSION   DATMJB010000433 DATMJB010000000
VERSION     DATMJB010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347788"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /gene="prpE"
                     /locus_tag="VNU48_09285"
                     /gene_synonym="yahU"
     CDS             <1..782
                     /gene="prpE"
                     /locus_tag="VNU48_09285"
                     /gene_synonym="yahU"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701987.1"
                     /note="catalyzes the formation of propionyl-CoA using
                     propionate as a substrate; PrpE from Ralstonia
                     solanacearum can produce acetyl-, propionyl-, butyryl- and
                     acrylyl-coenzyme A, and Salmonella enterica produces
                     propionyl- and butyryl-coenzyme A; not expressed in
                     Escherichia coli when grown on propionate/minimal media;
                     ATP-dependent; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HWI11508.1"
                     /translation="YWQTESGWPILTIANGVQALPSKFGSPGFAMYGYDVKVIDESTG
                     AEITTPNTKGVLAIAGPLPPGCMQTVWRDDERFVNTYWKDFAGQASHDKQQPGRLLYS
                     TFDWGIRDADGYFFILGRTDDVINVAGHRLGTREIEESISSHASVAEVAVVGVADALK
                     GQVAMAFVVPKDAAAVADATQRLKLEGEIMKTVDRQLGALARPARVHFVALLPKTRSG
                     KMLRRAIQAVCEHRDPGDLTTMDDPSALQQIKALVESPAAT"
     gene            798..2081
                     /locus_tag="VNU48_09290"
     CDS             798..2081
                     /locus_tag="VNU48_09290"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HWI11509.1"
                     /translation="MPDQAASPPIVPDEPLDATESDPNRLGWMEGSPPPPDKQVRFDD
                     EGMYSFPKLRWAYSNIRRLVPTTNVARGSGPVAELPRADRDDIDGLTFQPIGRSDTMS
                     WARSLAANYTDGIVVLHRGRIVHERYFGALDAQRAHIAWSVTKSFFGTIAASLIAEGQ
                     LDPAAPMSAYVPELAGSGLGNATLRQVLDMTTAIHYSEDYTDPEAGIWQHGRAGGILP
                     RTPAQIGPASFYEFLATLRQDGAHGQGFSYKTVNTDVLGWVLRRSTGQSLSELLSQRL
                     WSRLGAEQDGHLMVDPTGTEFAGGGFNCCLRDLARFGEMLRNDGVWNGQRVVPKAAID
                     DIRGGGDPAHFAQAGYVTLPGASYRDMWWVLHNEHGAFCARGIHGQGVYIDPKAEMVI
                     ARFASHHIAGNMGIDPTTLPAYHALAKFLLISSGA"
     gene            complement(2090..>2420)
                     /locus_tag="VNU48_09295"
     CDS             complement(2090..>2420)
                     /locus_tag="VNU48_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWI11510.1"
                     /translation="ATNDLVRSKIKIKSLNFMRGRTFLNKFVLIDEAQNLTPKQMKTL
                     ITRAGPGTKIVCLGNLAQIDTPYLTEGSSGLTFTVDRFKGWPHSGHVTLARGERSRLA
                     DFASEVL"
BASE COUNT          401 a          849 c          818 g          352 t
ORIGIN      
        1 actactggca gactgaatcc ggctggccga tcctgacgat cgcgaacggt gtccaggcgt
       61 tgccgagcaa gttcggcagc ccgggcttcg cgatgtacgg ctacgacgtg aaggtgatcg
      121 acgagtcgac cggcgccgag atcaccacgc cgaacacgaa gggcgtgctc gcgatcgccg
      181 gcccgctgcc gccgggctgc atgcagacgg tgtggcgcga cgatgagcgc ttcgtcaaca
      241 cgtactggaa ggacttcgcc gggcaagcaa gtcatgacaa acagcagcct ggtcggctgc
      301 tgtacagcac cttcgactgg ggcatccgcg acgccgacgg ctacttcttc atcctcggcc
      361 gcaccgacga cgtgatcaac gtcgccgggc atcgcctggg cacgcgcgag atcgaggaga
      421 gcatctccag ccacgcaagc gtggccgagg tcgcggtcgt cggcgtcgcc gatgcgctga
      481 agggccaggt cgcgatggcc ttcgtcgtgc cgaaggacgc ggcggccgtg gccgacgcaa
      541 cgcaacgtct gaagctcgaa ggcgagatca tgaagaccgt cgaccggcag ctcggcgcgc
      601 tcgcgcggcc ggcacgggtc cacttcgttg cgctgctgcc gaagacgcgc tccggcaaga
      661 tgctgcgtcg ggcgatccag gccgtctgcg agcatcgcga tccgggcgac ctgacgacga
      721 tggacgaccc ctccgcgctg cagcagatca aggcactcgt cgaatcaccc gccgccacct
      781 gaagcccggc cgccacgatg cccgaccaag ccgccagccc accgatcgtc ccggacgagc
      841 cgctggacgc caccgagagc gacccgaacc gcctcggctg gatggaaggg tcgccgccgc
      901 cgcccgacaa gcaggtccgc ttcgacgacg agggcatgta cagcttcccg aagctgcgct
      961 gggcctactc gaacatccgc cgtctggtgc cgacgaccaa cgtcgcgcgc ggcagcgggc
     1021 cggtcgcgga gctgccgcgc gccgaccgcg acgacatcga cggcctgacg ttccagccga
     1081 tcgggcgcag cgacacgatg agctgggccc ggtcgctcgc ggcaaactac accgacggca
     1141 tcgtcgtgct gcaccgtggc cgcatcgtcc acgagcgcta cttcggcgcg ctcgacgcgc
     1201 agcgcgcgca catcgcgtgg tcggtgacga agtcgttctt cggcacgatc gcggcgtcgc
     1261 tgatcgccga agggcagctc gaccctgccg cgcccatgag tgcgtacgtg cccgagctcg
     1321 ccggcagcgg cctgggcaac gccacgctgc gtcaggtgct ggacatgacc acggccatcc
     1381 actactcgga ggactacacc gaccccgagg ccggcatctg gcagcacggc cgcgccggtg
     1441 gcatcctgcc gcgcacgccc gcgcagatcg gcccagcgag tttctacgag ttcctcgcca
     1501 ccttgcggca ggacggcgcg cacggccagg gcttcagcta caagaccgta aacaccgatg
     1561 tgctcggctg ggtgctgcgc cgctccaccg gccagtcgct gagcgaactg ctgtcgcagc
     1621 gcctgtggtc acggctcggc gccgaacagg acggccatct gatggtcgac ccgaccggca
     1681 ccgagttcgc cggcggcggc ttcaactgct gtctgcgcga cctggcccgc ttcggcgaga
     1741 tgctgcgcaa cgacggcgtc tggaacggcc agcgcgtggt gccgaaggcc gcgatcgacg
     1801 acatccgcgg cggcggcgac ccggcgcatt tcgcgcaggc cggctacgtc acgctgccgg
     1861 gcgcaagcta ccgcgacatg tggtgggtgc tgcacaacga gcacggggcg ttctgcgcgc
     1921 gcggcatcca cgggcagggc gtgtacatcg acccgaaggc cgagatggtg atcgcgcgct
     1981 tcgcctcgca ccacatcgcc ggcaacatgg gcatcgaccc gaccaccttg cccgcgtacc
     2041 acgcgctcgc gaagttcctc ctgatcagct cgggggcttg agggccgcgc tacagcacct
     2101 cggaggcgaa gtccgccagg cgcgaacgct cgccgcgtgc cagcgtcacg tggccggaat
     2161 gcggccagcc cttgaagcgg tcgaccgtga aggtcaggcc ggaactgccc tcggtcaggt
     2221 acggcgtgtc gatctgcgcc aggttgccca ggcagacgat cttcgtgccc ggcccggcgc
     2281 gcgtgatcag cgtcttcatc tgcttgggcg tgaggttctg cgcctcgtcg atcagcacga
     2341 acttgttcag gaaggtgcgg ccgcgcatga agttcagcga cttgatcttg atcttgctgc
     2401 gcaccaggtc gttggtggcg
//
LOCUS       DATMJB010000434        11996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_198970, whole genome shotgun sequence.
ACCESSION   DATMJB010000434 DATMJB010000000
VERSION     DATMJB010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 11996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11996
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198970"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VNU48_09300"
     CDS             <1..886
                     /locus_tag="VNU48_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWI11511.1"
                     /translation="HGVGFGGMSVGGMVANRRTFGPMLPGVDHLRHTLDLERNAFTRG
                     QPQHGKELADDLERLCALHDPSTIAAVIVEPVAGSTGVLLPPKGYLQRLRAICDKHGI
                     LLIFDEVITGFGRLGSPFAADHFGVTPDLMTVAKGISNGCVPMGAVFAKQAIHDAFMT
                     GPEHLIEFPHGYTYSSHPLACAAALGTLDTYADEGLLTRAATMGPVFENALHSLKGVP
                     HVIDVRNIGLVGGIELDPIAGQPGKRAFDAFLECWERGVLIRTTGDTIALSPPLIVES
                     GHIDQMVSTIADVLKKHA"
     gene            924..1730
                     /locus_tag="VNU48_09305"
     CDS             924..1730
                     /locus_tag="VNU48_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HWI11512.1"
                     /translation="MTAAATAAKPKPVVLVPACNRMLGAHPFHVAGKKYVDAVRLAGC
                     TPLIVPTASIEELSDELDALLALADGVLLTGSASNVHPRHFAEDVHDPALPLDADRDA
                     WTLPLIPRALARGMPLFAICRGFQETNVALGGSLHQAVQEVAERDDHRAPKDAPPELQ
                     YGPAHDVIVQPGGVLASVLDETRIRVNSVHGQGVNRLADGLRVEALAPDGLVEAFSVA
                     DARGFNLGVQWHPEWQAAGDPVSMRLFKAFGAACEAYRDRHRVPHHEPDR"
     gene            1829..3196
                     /locus_tag="VNU48_09310"
     CDS             1829..3196
                     /locus_tag="VNU48_09310"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWI11513.1"
                     /translation="MQDKSKFTFADLEQWLNERRVTEIECLVPDLTGVARGKILPREK
                     FTEDRGMRLPEAIVGIGVTGNFPADGPYYDVIHPTDRDMHLRPDPSTVRIVPWAADPT
                     AQVIHDCFDRDGNMIPYAPRSVLRRICDLYAAEGWNPVVAPELEFYLIERNADPNTPL
                     RPPAGRSGRAETSRQAYSIDAVNEFDPLFEDIYAYCQQMELNVDTLIHEVGAGQMEIN
                     FFHDHPLGLADEVFFFKRTIRETALRHEMYATFMAKPMANEPGSAMHVHQSILSSVTG
                     KNIFSNEDGSPSKEFYWYIGGLQKYTPAAMALFAPYVNSYRRLARSTAAPINIQWGSD
                     NRTVGIRSPVATAASRRIENRVIGADANPYVALAATLACGYLGMKQQIEPAPECKGDA
                     YLSEYQLPRSLSEALGWLADEKDLHDVLGKEFITVYSEVKEIEHDEFMKVISPWEREH
                     LLLHV"
     gene            3210..4595
                     /locus_tag="VNU48_09315"
     CDS             3210..4595
                     /locus_tag="VNU48_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWI11514.1"
                     /translation="MSDPSTRSTKDWQAADAAHYLHPFTDFKALAAKGSRVIARADNI
                     YLWDTDGHRLLDTMSGLWCVNVGYGRQELIEAATKQMTELPFYNSFFQTATKPAIELA
                     ELLAEVTPPQFRHVFFSGSGSEGNDTVVRMVRRYWDVLGQPERQVIISRRNAYHGSTM
                     AGASLGGMSGMHAQGGLPIPGIVHIEQPFWHELGRDLDRDAFGLKAARWLEEKILEVG
                     ADRVAAFIGEPVQGAGGVIVPPDTYWPEVQRICDRYGVLLASDEVICGFGRTGHWFGC
                     ERFGFRPDLMTFAKGVTSGYIPLGGVMVGERIAKVLIEQGGEFNHGYTYSGHPVACAV
                     ALANIRLLQREHIVETVRDDTGPYLAQQFAALAVHPLVGEVQTCGLMGALQFVKDKST
                     GAPFPAEIELGMVCRGHCFGNGLIMRAVGDRMIVAPPLVITRAQIDEMMALVRRCLDL
                     TLADAVRHGWV"
     gene            4653..5762
                     /gene="potA"
                     /locus_tag="VNU48_09320"
     CDS             4653..5762
                     /gene="potA"
                     /locus_tag="VNU48_09320"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HWI11515.1"
                     /translation="MSTEAFLQIRDIVKDFGGHLAVNHVSVDIAKGEIFALLGSSGCG
                     KTTLLRMLAGFETPTAGSITLAGQDLAGLPPYERPLNMMFQSYALFPHLTVRDNIAFG
                     LKRDGLPAAEIAQRVDAMLELVQLEKFGQRKPHQLSGGQQQRVALARSLAKKPQLLLL
                     DEPLGALDKKLREETQIELVNIIHEVGVTCVMVTHDQEEAMTMASRIAVMSEGRFLQV
                     GVPGEIYETPANRFVADFIGNVNLMDGTLLKDEVDHVLVRCADCTHYVGHGITGTEGM
                     RVSVAVRPEKIRLSHDAPPAVAGETGAPFNSARGTVKDMAYFGSFTVYHLLLASGALL
                     KVSAGNTERHRDHAIGHGDAVWAHWSPTAQVVLTS"
     gene            5759..6685
                     /locus_tag="VNU48_09325"
     CDS             5759..6685
                     /locus_tag="VNU48_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWI11516.1"
                     /translation="MSAAASTQGARRGWAVIGVPYVWLLLSFLLPFLIVLKISVSEMD
                     IASVHDVITLQDGHLALDLKFGNYAALLQDDLYLLTYLASLKYAAITTVLCLGLGYPF
                     AYFMARARATIQPALLMLVMLPFWTSFLLRVYAWKALLTEHGWAFDAITGSGLDRMLA
                     SAGLIPAPGQLMNTPFSLVLGMTYTYLPFMILPLYSNLAKMDLRLLEAASDLGATAWV
                     AFWKITVPLSKAGIIAGAMLVFIPCVGEYVIPELLGGPQTQMIGRTLWDEFFSNNDWP
                     MASTVAVVMVLLVIVPLALFNKYQAEAVERRV"
     gene            6756..7598
                     /locus_tag="VNU48_09330"
     CDS             6756..7598
                     /locus_tag="VNU48_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWI11517.1"
                     /translation="MGALTFNRWFGRGWLSLGYAFLYLPIVALVIFSFNDSPVPNVWR
                     GFTLKWYASLSSDTEIIAGLWLSLRIAFFTACASVVIGTLAALALVKHRRFVGRTLFS
                     GMLSAPLVMPEVIIGLSLLLMLVSMQKALGFPERGLVTIWLGHLLLGLAYAAVVVQAR
                     LKDLNPQLEEAAMDLGARPSQVFWLVTLPMISQALLSAWLLTFTLSLDDVVLSAFLSG
                     PGATTMPLVIFSRARLGLNPSVNAIATLTVTLVSVGVVCASLLIARRERLRAAEIAAA
                     ARAG"
     gene            7616..8551
                     /locus_tag="VNU48_09335"
     CDS             7616..8551
                     /locus_tag="VNU48_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWI11518.1"
                     /translation="MPSGVGATPAHAAPHAPWLSFLCLAGSMALVGSYVGLSKILVLV
                     FPIFLLAFLRFGIAALAMAGWVRRAPQETRLSRHDRGLLFLESFFGNFLFSICMLFGV
                     SMTSALAAGVIMAGIPAAVALMSRAFLRERIAPRTMAGIACAVGGIALVSFTKNADAA
                     TPGSLLGNLLLLSAVLCEASYVVIGKKLTGNVSPKRISALINLWGLALITPFGLWQAL
                     RFDFGAVAAGSWWLLAFYSIAASMVTVWLWMTGLKHVPAASAGIFTVLLPVSAAAVGV
                     LFLGERFSAAQAAAFALALAGVVLATWPARAPASA"
     gene            complement(8561..9544)
                     /gene="dusA"
                     /locus_tag="VNU48_09340"
     CDS             complement(8561..9544)
                     /gene="dusA"
                     /locus_tag="VNU48_09340"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HWI11519.1"
                     /translation="MNQQLSPWRLCTAPMMDWTDRHCRYFHRLLTRRTRLYTEMVTTG
                     ALLHGDVPRHLDFSPEERPLALQLGGSEPAELARCAKLAEQWGYDEVNLNCGCPSERV
                     QRGSFGACLMAEPSLVADCVKAMRDASALPVTVKHRIGIDKVERYEFVRDFVGAVAAA
                     GCEVFIVHARNAWLKGVSPKENRELPPLRYELVHRLKREFPALTIVLNGGIKTNAQIA
                     EQLEHVDGVMLGREPYHHPWLMADWDARFFGAASVERERDAVEAQMVEYMQRIVAKGE
                     PWSHASRHLIGLRNGLAGARRWRQVWSDHRLKNVSPAEVSRLAHEGAEALA"
     gene            complement(9701..9946)
                     /locus_tag="VNU48_09345"
     CDS             complement(9701..9946)
                     /locus_tag="VNU48_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11520.1"
                     /translation="MQPFRRDLLAVDAGHVRTTPVAVADFLDGASERQVPKLNGRSLR
                     KPPPAAMGRKLTFGALIQSNESSHHHDRVPLRTLLGI"
     gene            10248..11624
                     /locus_tag="VNU48_09350"
     CDS             10248..11624
                     /locus_tag="VNU48_09350"
                     /inference="COORDINATES: protein motif:HMM:NF016847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWI11521.1"
                     /translation="MQQHAATFIAQAEDAAGADLPQFVKDEFDAFLECGILAHGFLRL
                     RCGVAFSCKRRGFCPSCGARRMAKTAAHLVDHVIPHVPVRQWVLSLPIPLRLLLAAQP
                     KLLTPVLQVVHRVITRFLLKQAGVKADEADSGAVTLIQRFGSAANLNIHLHCLVLDGV
                     YRRGTDGAPAFVGVPAPTDAALQTVLHKIITRMMKLLARRGVLVEEEGSTCMADGDSD
                     SDEARVLRPLQAAACTCRIAFGPRAGQKVLTVQGVMPKDADFKQTLCADSNGLSLHTA
                     VRCGADDRQALEQLCRYITRPALANERVQTNAAGQVVLKLKTPWRDGTTHLVMSPLEF
                     MQRRAALVPRPRLHLIRFHGVLAPNAKLRALAVQQEVEAPAQAAPHADCEANCAHHRP
                     VRLSWAKLLKRVFELDLEHCPNCGGELKIIAAILEQPVIEKVLMHLGLQARAPPRAPA
                     RGQALQAA"
BASE COUNT         1877 a         4134 c         4047 g         1938 t
ORIGIN      
        1 tcacggggtc ggcttcggcg gcatgtcggt cggcggcatg gtcgcgaacc ggcgcacctt
       61 cgggccgatg ctgccgggcg ttgatcacct gcgccacacg ctcgatctgg agcgcaacgc
      121 gttcacgcgc ggccagccgc agcacggcaa ggagctcgcg gacgatctgg agcgcttgtg
      181 cgcgctgcac gatccctcga ccatcgccgc ggtgatcgtc gagccggtcg ccggctcgac
      241 cggcgtgctg ctgccgccca agggctatct gcagcgcctg cgtgcgatct gcgacaagca
      301 cggcatcctg ctgatcttcg acgaggtgat caccgggttc gggcgcctgg gctcgccgtt
      361 cgccgccgat catttcggtg tcacgcccga cctgatgacg gtcgccaagg gcatcagcaa
      421 cggctgcgtg ccgatgggcg cggtgttcgc gaagcaggct atccacgatg cattcatgac
      481 cgggccggag cacctgatcg agttcccgca cggctacacc tattcgtcgc acccgctggc
      541 ctgcgcggcc gcgctcggca cgctcgacac ctacgccgac gagggcctgc tgacccgcgc
      601 ggcgacgatg gggccggtat tcgagaacgc gctgcattcg ctgaagggcg tgccgcacgt
      661 gatcgacgtg cgcaacatcg gcctggtcgg cggcatcgag ctcgatccga tcgcggggca
      721 gccgggcaag cgcgcgttcg acgccttcct cgagtgctgg gagcgcggcg tgctgatccg
      781 caccaccggc gacacgatcg cgctgtcgcc accgctgatc gtcgagagcg ggcacatcga
      841 ccagatggtc agcacgatcg ccgacgtgtt gaagaagcac gcttgagcga tatgctgcga
      901 gggtggacac gccctccttc ctgatgaccg cggccgcgac ggccgcgaag cccaagcccg
      961 tcgtgctggt gcctgcctgc aaccggatgc tcggcgcgca cccgttccat gtcgcgggca
     1021 agaagtacgt cgatgcggtc cgcctggccg gctgcacgcc gctgatcgtg ccgaccgcgt
     1081 cgatcgagga actcagcgac gaactcgatg cgctgctggc cctggccgac ggcgtgctgc
     1141 tcaccggttc ggcgtcgaac gtgcacccgc gccatttcgc cgaggatgtg cacgacccgg
     1201 cgctgccgct ggacgccgac cgcgacgcat ggaccctgcc gctgatcccg cgggcgttgg
     1261 cgcgcggcat gccgctgttc gcgatctgtc gcggcttcca ggagacgaat gtcgcgctcg
     1321 gcggctcgct gcaccaggcg gtgcaggagg tggccgagcg cgacgaccac cgcgcgccga
     1381 aagatgcgcc gcccgaactg cagtacgggc cggcccacga cgtgatcgtc cagccgggcg
     1441 gcgtgctggc gagcgttctc gacgagaccc gcatccgcgt caactcggtc cacggccaag
     1501 gggtgaaccg gctcgccgac gggctgcgtg tcgaggcgct ggcgcccgat ggtctggtcg
     1561 aggccttctc ggtcgccgac gcgcgcggct tcaacctcgg cgtgcaatgg catccggagt
     1621 ggcaggcggc cggcgatccg gtgtcgatgc gcctgttcaa ggccttcggc gcggcctgcg
     1681 aagcctaccg cgaccggcac cgcgtgccgc accacgagcc ggatcgatag cgccggcgcc
     1741 tgccaggagc cccgaagact ccgccgccga cgcaagaacc gattctcggt gcgaccgcca
     1801 cggttcgcac cccagcccca ggtgccccat gcaggacaag agcaagttca ctttcgccga
     1861 cctcgagcag tggctcaacg agcggcgcgt caccgagatc gaatgcctgg tgccggacct
     1921 gaccggcgtg gcccgcggca agatcctgcc gcgcgagaag ttcaccgagg accgcggcat
     1981 gcggctgccc gaggccatcg tcggcatcgg cgtgaccggc aacttccccg ccgacggccc
     2041 gtactacgac gtgatccacc cgaccgaccg cgacatgcac ctgcggcccg acccgagcac
     2101 cgtgcgcatc gtgccctggg ccgccgaccc gaccgcgcag gtgatccacg actgcttcga
     2161 ccgcgacggc aacatgatcc cgtatgcgcc gcgctcggtg ctgcgccgca tctgcgacct
     2221 gtacgcggcc gagggctgga acccggtcgt cgcgcccgaa ctcgagttct acctgatcga
     2281 gcgcaacgcc gatccgaaca cgccgctgcg cccgccggcc ggccgcagcg gccgcgccga
     2341 gacctcgcgg caggcgtact cgatcgacgc ggtgaacgag ttcgacccgc tcttcgaaga
     2401 catctacgcc tattgccagc agatggaact gaacgtcgac acgctgatcc acgaggtcgg
     2461 cgccggccag atggagatca actttttcca cgaccacccg ctcggcctcg ccgacgaggt
     2521 gttcttcttc aagcgcacga tccgcgagac cgcgctgcgc cacgagatgt acgccacctt
     2581 catggccaag ccgatggcca acgagcccgg cagcgcgatg cacgtgcacc agagcatcct
     2641 gagcagcgtg accggcaaga acatcttcag caacgaggac ggctcgccga gcaaggagtt
     2701 ctactggtac atcggcgggc tgcagaagta cacgccggcg gcgatggcgc tgttcgcgcc
     2761 ctacgtcaac tcgtaccggc gcctcgcgcg ctcgaccgcc gcgccgatca acatccagtg
     2821 gggcagcgac aaccgcaccg tcggcatccg ctcgccggtg gccacggccg cgtcgcgccg
     2881 gatcgagaac cgcgtgatcg gggccgatgc caacccgtat gtcgcgctgg ccgcgacgct
     2941 ggcctgcggc tacctcggca tgaagcagca gatcgagccg gcgcccgaat gcaagggcga
     3001 cgcgtacctc tccgagtacc agctgccgcg ctcgctgagc gaagcgctcg gctggctcgc
     3061 cgacgagaag gacctgcacg acgtgctcgg caaggagttc atcacggtct actccgaggt
     3121 caaggagatc gagcacgacg agttcatgaa ggtgatctcg ccgtgggagc gggaacactt
     3181 gttgctgcat gtctgacggc gaggccttga tgagcgaccc gagcacgaga agcacgaaag
     3241 actggcaggc cgccgacgcg gcgcactacc tgcatccgtt caccgacttc aaggcactcg
     3301 ccgcgaaggg ctcgcgcgtg atcgcgcgcg ccgacaacat ctacctctgg gacaccgacg
     3361 gccacagact cctcgacacg atgtcgggcc tgtggtgcgt caacgtcggc tacggccgcc
     3421 aggagctgat tgaagcggcg accaagcaga tgaccgagct gccgttctac aacagcttct
     3481 tccagaccgc gaccaagccg gccatcgagc tcgccgagct gctggccgag gtgacgccgc
     3541 cgcagttccg gcacgtgttc ttctcgggct ccggctccga gggcaacgac accgtcgtgc
     3601 gcatggtgcg ccgctactgg gacgtgctcg gccagcccga acgccaggtc atcatcagcc
     3661 gccgcaacgc gtaccacggc tcgacgatgg ccggcgcatc gctgggcggc atgagcggca
     3721 tgcacgcgca gggcggcttg ccgatccccg gcatcgtgca catcgagcag ccgttctggc
     3781 acgaactcgg ccgcgacctc gaccgcgacg cgttcggcct gaaggccgcg cgctggctcg
     3841 aggagaagat tctcgaggtc ggggccgaca gggtcgccgc cttcatcggc gaaccggtgc
     3901 aaggcgccgg cggcgtgatc gtgccgcctg atacttactg gcccgaggtc cagcgcatct
     3961 gcgaccggta cggcgtgctg ctcgcgtccg acgaagtgat ctgcggcttc ggccgcaccg
     4021 ggcactggtt cggctgcgag cgcttcggct tccggcccga tctgatgacc ttcgcgaaag
     4081 gcgtgacctc cggctacatc cccttgggcg gcgtgatggt gggcgagcgc atcgcgaaag
     4141 tgctgatcga gcaaggcggc gagttcaacc acggctacac ctactcgggc cacccggtcg
     4201 cctgcgcggt ggcgctggcg aacatccggc tgctgcaacg cgagcacatc gtcgagacgg
     4261 tgcgcgacga caccgggcca tacctcgcgc agcagttcgc cgctttggcc gtgcacccgc
     4321 tcgtcggcga ggtgcagacc tgcgggctga tgggcgcgct gcagttcgtg aaggacaagt
     4381 ccaccggcgc accgttcccg gccgagatcg agctcggcat ggtctgtcgc gggcactgct
     4441 tcggcaacgg cctgatcatg cgtgcggtcg gcgaccggat gatcgtcgcg ccgccgctgg
     4501 tgatcacgcg cgcgcagatc gacgagatga tggcgctggt acgccgctgc ctcgacctga
     4561 cgctggccga cgcagtgcgc catggctggg tctgagcggg cgggccgttt cgctagactg
     4621 ccgggtttgc gcgcccctca cccagctgtt caatgagcac cgaagcgttc ctgcagatcc
     4681 gcgacatcgt gaaggatttc ggcggccacc tcgccgtcaa ccatgtcagt gtggacatcg
     4741 cgaagggcga gatcttcgcg ctgctcggct ccagcggctg cggcaagacg acgctgctgc
     4801 gcatgctcgc cggcttcgag acgccgaccg ccggcagcat cacgctggcc ggtcaagacc
     4861 tcgccggcct gccgccttac gagcgaccgc tgaacatgat gttccagagc tacgcgctgt
     4921 tccctcacct gacggtgcgc gacaacatcg cgttcggcct gaagcgcgac ggcctgcccg
     4981 ccgccgaaat cgcgcagcgt gtggacgcga tgctcgagct cgtgcagctc gagaagttcg
     5041 gccagcgcaa gccgcaccag ctctcgggcg gccagcagca gcgcgtcgcg ctggcgcgca
     5101 gcctggcgaa gaagccgcag ctgctgctgc tcgacgaacc gctcggcgcg ctcgacaaga
     5161 agctgcgcga ggagacgcag atcgaactgg tcaacatcat ccacgaggtc ggcgtgacct
     5221 gcgtgatggt cacgcacgac caggaagagg cgatgacgat ggcctcgcgc atcgcggtga
     5281 tgagcgaggg ccgcttcctg caggtcggcg tgcccggcga gatctacgag acacccgcga
     5341 accgcttcgt cgccgacttc atcggcaacg tcaacctgat ggatggcacg ctgctgaagg
     5401 acgaggtcga ccacgtgctg gtgcgctgcg ccgactgcac gcactatgtc gggcacggca
     5461 tcaccggcac cgaaggcatg cgcgtgtcgg tcgcggtgcg gcccgagaag atccgtcttt
     5521 cgcacgatgc cccgccggcg gtcgcgggcg agaccggtgc gccgttcaac agcgcacgcg
     5581 gcacggtgaa ggacatggcc tacttcggca gcttcacggt ctaccacctg ctgctcgcca
     5641 gcggcgcgct gctgaaggtc agcgccggca acaccgagcg ccatcgcgac cacgcgatcg
     5701 gccacggcga tgcggtctgg gcccactggt cgccgaccgc gcaggtggtg ctgacctcat
     5761 gagcgctgcc gcatcgacgc aaggcgcacg tcgcggctgg gccgtgatcg gcgtgcccta
     5821 cgtctggctg ctgctgtcgt tcctgctgcc gttcctgatc gtgctgaaga tcagcgtctc
     5881 ggagatggac atcgccagcg tgcacgacgt gatcacgctg caggacggcc acctcgcgct
     5941 ggacctgaag ttcggcaact acgccgcgct gctgcaggac gacctgtacc tgctgaccta
     6001 cctggcgagc ctgaagtacg cggcgatcac gacggtgctg tgcctgggcc tgggctaccc
     6061 gttcgcgtac ttcatggcgc gtgcccgcgc gacgatccag ccggccctgt tgatgctggt
     6121 gatgctgccg ttctggacct cgttcctgct gcgcgtctac gcctggaagg cgctgctgac
     6181 cgagcacggc tgggccttcg acgcgatcac cggcagcggc ctggaccgca tgctcgccag
     6241 tgccggcctg atcccggcgc cgggccagct gatgaacacg ccgttctcgc tggtgctggg
     6301 catgacctac acctacctgc ccttcatgat cctgccgctg tactcgaacc tcgcgaagat
     6361 ggacctgcgg ctgctcgagg ccgccagcga tctcggcgcg actgcctggg tcgcgttctg
     6421 gaagatcacg gtgccgctgt ccaaggccgg catcatcgcg ggggctatgc tggtgttcat
     6481 cccctgcgtg ggcgagtacg tgatcccgga actgctcggc ggcccgcaga cgcagatgat
     6541 cggccgcacg ctgtgggacg agttcttcag caacaacgat tggccgatgg cctcgaccgt
     6601 cgcggtcgtg atggtgctgc tggtgatcgt gccgctggcg ctgttcaaca agtaccaggc
     6661 cgaagccgtg gagcggcgcg tgtgagcgcc gccagcccgg cgccgggccg ccccaaggcg
     6721 ggctgcgccc cctcgggggg caggagcgaa gcgacgtggg ggctctgact ttcaatcgct
     6781 ggttcgggcg tggctggctg tcgctcgggt acgcgttctt gtacctcccg atcgtcgcgc
     6841 tggtgatctt ctcgttcaac gattcgccgg tgcccaacgt ctggcgcggc ttcacgctga
     6901 agtggtacgc gtcgctgagc agcgacaccg agatcatcgc cggcctgtgg ctgtcgctgc
     6961 gcatcgcgtt cttcaccgcc tgcgcgtcgg tggtgatcgg cacgctcgcg gcgctcgcgc
     7021 tggtcaagca ccggcgcttc gtcgggcgca ccttgttctc gggcatgctg agcgcgccgc
     7081 tggtgatgcc cgaggtgatc atcgggctgt cgctgctgct gatgctggtc tcgatgcaga
     7141 aggccctcgg cttccccgag cgcggcctgg tgacgatctg gctcggccat ctgctgctcg
     7201 gcctggccta cgcggccgtc gtggtgcagg cgcggctcaa ggacctgaac ccgcagctcg
     7261 aagaggccgc gatggacctg ggcgcgcgac cgtcgcaggt gttctggctc gtgaccctgc
     7321 cgatgatctc gcaggcgctg ctgtcggcct ggctgctgac cttcacgctg tcgctggacg
     7381 acgtcgtgct ctcggccttc ctctccggcc ccggcgcgac gacgatgccg ctggtgatct
     7441 tctcgcgcgc ccgcctcggc ctgaacccga gcgtgaacgc gatcgcgacg ctgacggtca
     7501 cgctggtctc ggtcggcgtg gtctgcgcca gcctgttgat cgcgcggcgc gaacgcctgc
     7561 gcgccgccga gatcgcggcc gccgcgcgcg cgggttgacg ccgttggcgg atcccttgcc
     7621 ctcgggtgtc ggcgcgacgc ccgcccacgc cgcaccgcat gcaccctggc tgtcgttcct
     7681 gtgcctggcc ggcagcatgg cgctggtcgg cagctatgtc ggcttgtcga agatcctcgt
     7741 cctcgtgttt ccgatcttcc tgctcgcgtt cctgcgcttc ggcatcgccg cgctggcgat
     7801 ggccggctgg gtccggcgcg ctccgcaaga gacacggctg agccggcacg atcgcggctt
     7861 gctgtttctc gaatcgttct tcggcaactt cctgttctcg atctgcatgc tgttcggcgt
     7921 ctcgatgacc tcggcgctcg ccgccggcgt gatcatggcg ggcatcccgg cggcggtcgc
     7981 gctgatgtcg cgcgcattcc tgagggaacg catcgcgccg cgcacgatgg ccggcatcgc
     8041 ctgcgccgtc ggcgggatcg cgctggtgtc gttcacgaag aacgccgatg cggcgacgcc
     8101 cggctcgctg ctgggcaacc tgctgctgct cagcgcggtg ctgtgcgagg ccagctatgt
     8161 cgtgatcggc aagaagctca ccggcaacgt cagcccgaag cgcatcagcg cgctgatcaa
     8221 tctgtggggc ctggcgctga tcacgccgtt cgggctgtgg caggcgctgc gcttcgactt
     8281 cggcgcggtg gcggccgggt cgtggtggct gctcgcgttc tattcgatcg ccgcgagcat
     8341 ggtgacggtc tggctgtgga tgaccgggct gaagcacgtg ccggccgcgt cggcgggcat
     8401 cttcacggtg ctgctgccgg tcagcgccgc ggcggtcggc gtgctgttcc tcggcgagcg
     8461 cttcagcgcc gcacaggccg cggcgttcgc actggcgctg gccggtgtcg tgctcgccac
     8521 ctggccggcg cgcgccccgg cgagcgcgtg agagaagcgc tcaggccagc gcctcggcgc
     8581 cttcgtgcgc gagccgactg acctcggccg gcgacacgtt cttgagccgg tgatccgacc
     8641 acacctggcg ccagcgacgc gcaccggcca gcccgttgcg cagcccgatc agatggcgcg
     8701 acgcatgcga ccagggctcg cccttcgcga cgatgcgctg catgtactcg accatctgcg
     8761 cctcgaccgc gtcgcgctcg cgctcgaccg atgcggcgcc gaagaagcgc gcgtcccagt
     8821 ccgccatcag ccacgggtgg tgatagggct cgcggccgag catcacgccg tcgacgtgct
     8881 cgagctgctc ggcgatctgc gcgttcgtct tgatgccgcc gttgagcacg atggtcagcg
     8941 ccgggaactc gcgcttgagg cggtgcacga gttcgtagcg cagcggcggc agctcgcggt
     9001 tctccttcgg gctgacgccc ttcagccagg cgttgcgcgc gtgcacgatg aagacctcgc
     9061 aaccggccgc cgcgaccgca ccgacgaagt cgcgcacgaa ctcgtagcgc tcgaccttgt
     9121 cgatgccgat gcggtgcttg accgtcaccg gcagcgcgct tgcatcgcgc atcgccttca
     9181 cgcaatcggc gacgagcgaa ggctcggcca tcaggcaggc gccgaaggag ccgcgctgca
     9241 cgcgctcgct cgggcagccg cagttcaggt tgacctcgtc gtagccccat tgctcggcca
     9301 gcttcgcgca gcgcgccagc tcggccggct cgctgccgcc gagctgcagc gcgagcgggc
     9361 gttcctcggg gctgaagtcc aggtgccgcg gcacgtcgcc gtgcagcagc gcgccggtcg
     9421 tcaccatctc ggtgtacagc cgggtgcggc gcgtcagcag gcggtggaag tagcggcaat
     9481 ggcggtcggt ccagtccatc atgggtgccg tacacaggcg ccatggacta agttgttgat
     9541 tcatatgcat taatcgtcag tctatcagtc cagttggaat gggacagacc acatgaagtc
     9601 ttgaacggca ggaggaccat ttctccatct tgatcagtgg gttgtgcgca ggggtcccgc
     9661 caaagcacga cgctcggacg cccgctgctc gatggaccgc tcaaataccg agcaacgtcc
     9721 tgagtggcac gcggtcatgg tggtgactgc tttcgttcga ctgaatcagc gcgccgaagg
     9781 tcaacttgcg acccattgca gccggcggcg gttttctcag cgagcgaccg ttcaacttcg
     9841 gaacttgacg ttcgctcgca ccgtcgagaa agtcagcgac ggcgacggga gtcgttcgaa
     9901 cgtggccagc atcgaccgca agaagatccc ggcggaacgg ctgcatccga agaccgtgta
     9961 ctcacactgg cgaccctgaa gagacatcca ctgccctgat ctgggcgccc caaagctgcc
    10021 agtcgaaccc acacacaggc acccgttggc ggccccgcag cacagggcac gcatcggcag
    10081 gaagcactct gggcgattga ggcgcagtgc acggcttgag accgtgcaga ctcaccactc
    10141 gggagcctcg cgtgcaagcc atcggccggc agccccagag ggcgccggat ggcacccggg
    10201 tccattacga gcgccaccgc ccggagcaga ccacgctgta ccgcctggtg cagcagcacg
    10261 ctgcgacctt catcgcgcag gccgaagacg ctgcaggcgc cgacctgccg caattcgtca
    10321 aggacgagtt cgacgcgttc ctcgagtgcg gcatcctggc ccacggcttc ctgcgcctgc
    10381 gctgcggggt cgccttcagt tgcaaacggc gcggcttttg cccgtcgtgc ggagcccggc
    10441 gcatggcaaa gaccgccgct cacttggtgg accacgtcat cccccatgtg ccggtacgcc
    10501 agtgggtgct gtcgctgccg atcccgttgc gcctgctcct ggccgcacaa cccaagctgc
    10561 tgacgcccgt gctgcaggtc gtacaccgcg tgatcacgcg gttcctgctc aagcaggcgg
    10621 gcgtgaaggc agacgaggcc gacagcggcg cagtcacgct gatccaacgc ttcggttcgg
    10681 ccgccaacct caacattcac ctgcactgcc tggtgctgga cggtgtgtac cggcgcggca
    10741 ccgatggcgc gccggcattc gtcggagtac ctgcgccgac cgacgcagcg ctgcagacgg
    10801 tgttgcacaa gatcatcacc cgcatgatga agctgctcgc ccgtcggggg gtgttggtcg
    10861 aagaggaggg ttcgacctgc atggccgacg gcgacagcga ctccgatgag gcccgcgtgc
    10921 tcaggccgct gcaggcggcg gcctgcactt gccgcatcgc cttcggcccg cgcgctgggc
    10981 agaaggtgct gacggtccag ggcgtgatgc ccaaggatgc ggacttcaag cagacgctat
    11041 gtgccgacag caacggcctc agcctgcaca ccgcggtgcg ctgcggtgcc gacgaccgcc
    11101 aggcgctgga gcaactgtgc cgctacatca cacgcccggc gctggccaac gaacgcgtac
    11161 aaaccaacgc cgctggccag gtcgtgctga agctgaagac cccttggcgc gacggcacca
    11221 cccacctggt gatgtcgccg ctggagttca tgcagcggcg ggcggccctg gtgcccaggc
    11281 cgaggctgca tctgatccgc ttccacggcg tcctggcgcc caacgccaag ctgcgcgcgc
    11341 tggcggtgca gcaagaggtc gaggcgcccg cgcaggccgc gccacacgcc gattgcgagg
    11401 cgaattgtgc gcaccaccgc ccggtgcggc tgagctgggc caagctactc aagcgtgtgt
    11461 tcgagctcga cttggagcat tgcccgaact gcggcggcga gctcaagatc attgcggcca
    11521 tcctggagca accggtgatc gagaaggtcc tcatgcacct ggggctgcag gcgcgggcac
    11581 cccctcgggc gccagcccgt ggccaggcgc tacaagcggc ctgacgctgc ccaaccttga
    11641 ctgttcgggc gacccgatgc acggggccgc tggagtcggc tgcgtccgag ggcttgcggg
    11701 accgatgaaa gcggcctggt gacaagggac aacccccaga cagcgcccga gaaagacgac
    11761 tttcggctgc gctgtcaacg ctcaacggat ctctgcgacc ttcaaagacc gactcaagcg
    11821 cgtcgggcgc cgtgttcggc gtgccacggg gaaggaaaaa gggcgtttga gcctgtccgg
    11881 aattttgtgt gcggggcata gcatgaccaa gaggagcatg tatgccaacc agcaggaaga
    11941 cgacgaaggc ggccatggcc gcagcggcgg cgatgccgtc gatccccaag gaactc
//
LOCUS       DATMJB010000435        10958 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_420582, whole genome shotgun sequence.
ACCESSION   DATMJB010000435 DATMJB010000000
VERSION     DATMJB010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10958
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420582"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="VNU48_09355"
     CDS             <1..901
                     /locus_tag="VNU48_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HWI11522.1"
                     /translation="AEQRLVASAVLAKCDALDGTSDGLVQDTAACQAAFNLDRDVPTC
                     SGARDGSCLSAAQKLVIAKRFSGETTSAGTTFYSSWPFDAGIGSGNTAFWNFTAPLVL
                     DSGAVGFIWQVPPENPATFNGPAFALSANVDTMLAKIQATNATYTESALSFMTPPNPS
                     KLGTLKNRGAKMMVYHGTSDPIFSSDDTTAWYESLRTANGGDAANFARFYRVPGMTHC
                     SGGPSTDQFDMLTPLVAWVEQGTAPDSVVASARGAGNAAAANPDVPADWSATRTRPLC
                     AYPKVARYNGSGSIESAGSFSCQ"
     gene            927..1790
                     /locus_tag="VNU48_09360"
     CDS             927..1790
                     /locus_tag="VNU48_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWI11523.1"
                     /translation="MNHAELIAIDTHTHLEVSCWNPFDNYGEEYDRAADKYFRSSRRP
                     TMAESLAYYREKKIGFVMFTVDAETQMGRRRIPNEEIAEAAQANSDIMIAFGSIDPHK
                     GKMGAREARKLIEEHGVKGFKFHPTLQGFEPYDKMAWPIYEVIAEYKLPAIFHSGHSG
                     IGSGMRCGGGLRLQNSNPMLLEDVAIDFPDIEIVVAHPSWPWQDEALSLAMHKPNVWI
                     DLSGWSPKYFPPQLVQYANTQLKDRILFGSDYPLITPDRWMKDFAEAGFKPEVQPLIL
                     KENAKRLLRLT"
     gene            1867..2520
                     /locus_tag="VNU48_09365"
     CDS             1867..2520
                     /locus_tag="VNU48_09365"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWI11524.1"
                     /translation="MSLRINDTAPDFEAETTQGKIRFHEWIGEQWAVLFSHPKDFTPV
                     CTTELGYMAKIEPEFSKRNAKLIGLSVDPVARHAEWSKDIEETQGHAPKYPMIGDADL
                     KVAKLYNMLPADEVGSDGRTAANNATVRSVFIIGPDKKIKLMLTYPMTTGRNFDEILR
                     VLDSMQLTAKYKVATPVNWKHGEDVIITPAVSNEEAAKSFPGFKTIKPYLRTTKQPG"
     gene            2552..3640
                     /locus_tag="VNU48_09370"
     CDS             2552..3640
                     /locus_tag="VNU48_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWI11525.1"
                     /translation="MLVVRQLFDPQSSTYTYLLGDSASGEALLIDPVFEHVRRDTALL
                     RELGLHAVATLDTHVHADHVTGAWLHKVGSGSRIMVSEASAAANADRLLRHGDRVSFG
                     ARHVEVRATPGHTNGCLTYVLDDQSMAFTGDSLLIRGCGRTDFQHGSPARLYRSVWEQ
                     ILSLQPGCLLYPGHDYRGLTVTSVAEEKRFNPRLGGDIDEADFAGYMNNLGLPHPKLM
                     DIAVPANLRCGQPDQTAPQSDGPSWAPLSCNFGGIWEIQPEALEECLAGGRGTDIQVI
                     DVREPREFTGGLGHIPGARLVPLAELGARSAEFDKARPVVTVCRSGARSAQATVLLQK
                     SGFSQVANLAGGMLRWRAEALPFNRGAG"
     gene            complement(3701..4534)
                     /gene="cobA"
                     /locus_tag="VNU48_09375"
     CDS             complement(3701..4534)
                     /gene="cobA"
                     /locus_tag="VNU48_09375"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWI11526.1"
                     /translation="MSDAPDIEETARGRHAIAAASGQVTLVGAGPGDPELLTLKAVRA
                     IRQATLLLVDDLVGDAVLRYARRSARIIHVGKRGGCASTPQAFIERLMAAEALNGERV
                     VRLKGGDPFVFGRGGEEAAHLRALGLRVEVVNGITAGLAAATSIGVPLTHRDHAHGVI
                     FVTGHARGDAAPLDWAALAAAAAQGLTLVIYMGIATVEAIESGLLQGLPAATPVAIVQ
                     HASLARQRHACCALSALAHTVAREGLSSPAVIVVGDVAAAARELSAPRALDEARARRL
                     A"
     gene            complement(4531..4917)
                     /locus_tag="VNU48_09380"
     CDS             complement(4531..4917)
                     /locus_tag="VNU48_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11527.1"
                     /translation="MAMQLDDLLGKTCVIGLSYFDLQGGLMKQTQYAGAVVKVDAEQG
                     ISVRLAHADASVAQADFIVPPNLDAWFKAPPGHYRHAPSGVDIHDPDFVVTWDIHRTQ
                     TNAADGQHEWWDWVPNLVPPRVGTPG"
     gene            complement(4920..6887)
                     /locus_tag="VNU48_09385"
     CDS             complement(4920..6887)
                     /locus_tag="VNU48_09385"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWI11528.1"
                     /translation="MKNDLLPLDSLVSEAIKSAARPRKIWEIRTEEQSYAFLRDTNIA
                     EANRYGQKIDLIELAGQKLPAGRSMFINENPAVGTNPNRNKQHGFFFTADNCIGCHAC
                     EAACSEKNDNPAHLAFRSVGYVEGGSFPDYKRMNISMACNHCDDPVCLKGCPTRAYTK
                     HAEYGAVLQDPETCFGCGYCTWVCPYNAPQLDPVKGQVSKCNMCVDRLEVGLKPACVS
                     ACVGNALDFGVIEKVPEGREQARVEIPGFPSPEITRPNIRFQQIKQLPDEMMRTDSMP
                     LKYHKDEHGRYRPAIDQKKGKAMHWNLARLSSRENPLVMFTLAAQAAAGAFALQFFGA
                     QFGIGGFVAFAQSALYAPLAILEFMLVAFGLFMSTMHLGKPHRFYRGFNNLRHSPVSR
                     EGLGIAIFIGALGMHILFSLPANAWFQALCSGLVGVDVSRLPGAAAAPRLATIFGALA
                     VLGSVAGLYYMNRCYRIKARPFWDHWQVTTAFVGSALSLGALLTGAVALATLGLAGES
                     VVATAWVCGGLLTLGLAIEGVGHVAHARVMGRAEHEGGASYYVQTTTFGKTFMLRNTL
                     LGANGVLACGLLVAMLLQGGLGPVTWLGWALVGATALLTSVVSRALFYVLVIPTTMPG
                     AFFWKNKGFEEHARDIGLANMPQVGVAPLRH"
     gene            complement(6906..9152)
                     /locus_tag="VNU48_09390"
     CDS             complement(6906..9152)
                     /locus_tag="VNU48_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HWI11529.1"
                     /translation="MLFGRTRRKPIAIPVKEVKEWKYTTCNYCSTGCAIEIGLNESGQ
                     IVTSRGHAGADVNRGKLCIKGLLEHELFTSPGRGRVPLMRDRAHQAFQPASWDAALDQ
                     TAAKIKEIQAKYGRDSFAVVSTGQLLTEEFYTLGKLVRGQIGTNSYDGNTTLCMASAV
                     SGYKRSFGSDGPPGCYGDFEHTHCLMAFGSNLPEQHPIIYWRLKEALEKRKFPLIVVD
                     PRVTMLAQFADIHLPITPGTDTVLINAMMHVIFKEGLQDDAYIANHTQGIDGLRALLA
                     DYDPKTAQHVCGIDEDRIRTVARIYAKAPAAMSIWTMGINQSTHGSDGVVNINNLNLI
                     TGNIGIPGGTSLSITGQCNAMGTREWSSCSGLPGYRLLEKEKDRKVVADFWGIDESFF
                     PAARGLAQTDIFPAIETGEIKGLWLVATNPMTSMANQPRIVKAMENLEFLVVQDVYED
                     VETNKYSHVFLPAAVWAEKEGCHTNTERRVNLTRNVLPPYADSKPDFWIFNEMAKRFE
                     DRNKIHFPATPEGAFDEMRQLSKGEGRTLDISGMTYDRIEAARGIQWPYSEAQASLDT
                     ATIGPTAGFDMLHRQDFLGNPRLYTDGKFQTPDGHAKLIPVRFVNNNECPDAEYPFWL
                     NSGRVVEHFHTRTRTGKIGNCNKFSPTPYMEMNPDAAAELGIEHQSYVRVLSRRGDAV
                     VMVQLTQRVGRDMLFIPFHFHDCVNRLTLGLLDPHSRQPAFKQNAARVEKVDQAEAAR
                     LNLERRAF"
     gene            complement(9170..9550)
                     /gene="nirD"
                     /locus_tag="VNU48_09395"
     CDS             complement(9170..9550)
                     /gene="nirD"
                     /locus_tag="VNU48_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HWI11530.1"
                     /translation="MSNWKSICTLTDIPVLGARRVARADAIAVAVFRNAEDKVFALLD
                     RCPHKGGPLSQGIVFGESVACPLHNWTIGLADGCAKAPDEGCTVSFACKVEGGEVFLD
                     SVELATRALDAVAPVAGPRARTTA"
     gene            complement(9582..>10958)
                     /locus_tag="VNU48_09400"
     CDS             complement(9582..>10958)
                     /locus_tag="VNU48_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWI11531.1"
                     /translation="VYKKLVIKDDKLIGACLYGDTVDGSWYFKLLRDGRKVSDIRDRL
                     MFGESNIGDTGHEGHSKAAAMADADEVCGCNGVNKGTICKAIKEKGLFTLEEVKKHTK
                     ASASCGSCTGLVEQLLMFTAGGDYSKAPTKKPMCACTDASHQDVRESIRNDRLLSIDG
                     VYKAMGWRTPNGCSSCRPAVNYYLISTWPKEARDDPQSRFINERSHANIQKDGTYSVI
                     PRMWGGETTASELRRIADAVDKYKIPTVKVTGGQRIDLLGVKKEDLVNVWKDIGMPSG
                     HAYAKALRTVKTCVGSEWCRMGTQDSTQMGKDLERAMWRMYAPHKVKFAVSGCPRNCA
                     EAGIKDVGIIGVDSGWEMYVAGNGGIKTEVAHFFVKLKTAAEVLEYTGAFCELYRQEG
                     WYLERTVHYVSRVGLDYVKARILDDAAGRKALWANLQAALEGEPDPWFEFDKATVDTR
                     QFNPVAVP"
BASE COUNT         1725 a         3655 c         3766 g         1812 t
ORIGIN      
        1 cgccgaacaa cggctcgtcg cgagcgccgt tctggcgaaa tgcgatgcgc tcgacggtac
       61 gagcgacggc ctggtgcagg acacggccgc ctgccaggcc gccttcaacc tcgaccggga
      121 cgtgcccacc tgctccggcg cgcgcgacgg cagctgcctg tccgccgcac agaaactcgt
      181 gatcgccaag cgcttctcgg gcgaaaccac cagcgccggc accacgttct attcgagctg
      241 gcccttcgat gcggggatcg gcagcggcaa cacggcgttc tggaacttca cggcgccgct
      301 ggtgctcgac tcgggcgcgg ttggcttcat ctggcaggtc ccgccggaga acccggccac
      361 gttcaacggc ccggccttcg cgctgagcgc caacgtcgac accatgctcg cgaagatcca
      421 ggcgacgaac gcgacctaca ccgaaagcgc actgtcgttc atgacaccgc cgaacccgag
      481 caagctcggc acgctgaaga accgcggcgc gaagatgatg gtctaccacg gcaccagcga
      541 cccgatcttc tcgagcgacg acacgaccgc ctggtacgag tcgctgcgca ccgccaacgg
      601 cggtgacgcc gcgaacttcg cgcgctttta ccgtgtcccc ggcatgacgc actgctccgg
      661 cggcccgagc acggaccagt tcgacatgct gaccccgttg gtcgcgtggg tcgagcaggg
      721 cacggccccc gacagcgtcg tggcctcggc gcgcggcgcc ggcaatgccg ccgccgcgaa
      781 cccggacgtg ccggccgatt ggtcggcgac gcgcacccgg ccgctgtgtg cctacccgaa
      841 ggtggcgcgc tacaacggaa gcggtagcat cgagtcggcg ggcagcttca gctgtcaatg
      901 agcgagcgtt gacgagagag acctcgatga accacgccga gctgatcgcg atcgacaccc
      961 acacccacct cgaggtgtcg tgctggaacc cgttcgacaa ctacggcgag gagtacgacc
     1021 gcgccgcgga caagtacttc cgttccagcc ggcggccgac gatggccgag tcgctcgcgt
     1081 actaccgcga gaagaagatc ggcttcgtga tgttcacggt cgacgccgag acgcagatgg
     1141 gccgccgtcg catcccgaac gaggagatcg ccgaggccgc gcaggccaac agcgacatca
     1201 tgatcgcgtt cggcagcatc gacccgcaca aaggcaagat gggcgcgcgc gaggcgcgca
     1261 agctgatcga ggagcacggc gtcaagggct tcaagttcca cccgacgttg cagggcttcg
     1321 agccctacga caagatggcc tggccgatct acgaggtgat cgccgagtac aagctgccgg
     1381 cgatcttcca cagcggccac tcgggcatcg gcagcggcat gcgctgcggc gggggcttga
     1441 ggctgcagaa cagcaacccg atgttgctgg aagacgtggc gatcgacttc cccgacatcg
     1501 agatcgtcgt cgcacacccg agctggccgt ggcaggacga agcgctgtcg ctggccatgc
     1561 acaagcccaa tgtctggatc gacctctcgg gctggagccc gaagtacttt ccgccgcaac
     1621 tggtgcagta cgcgaacacg cagctgaagg accgcatcct gttcggcagc gactacccgc
     1681 tgatcacacc ggaccgctgg atgaaggact tcgccgaggc cggcttcaag cccgaggtgc
     1741 agccgctgat cctgaaggag aacgcgaagc gcctgttgcg gctgacgtga gccgcggctc
     1801 gacgtagact gtcgagcccg gcgtcgaaag gcgcgacgcc gcggccccaa caaggaggtc
     1861 cccaccatgt cactgcgcat caacgacacc gcccccgact tcgaggccga gaccacgcaa
     1921 ggcaagatcc gctttcacga gtggatcggc gagcagtggg cggtcctgtt ctcgcacccg
     1981 aaggacttca cgccggtgtg caccaccgaa ctcggctaca tggccaagat cgagcccgag
     2041 ttcagcaagc gcaacgccaa gctgatcggg ctgagcgtcg acccggtggc gcgccacgcg
     2101 gagtggtcca aggacatcga ggaaacgcag ggccatgcgc cgaagtaccc gatgatcggc
     2161 gacgccgacc tgaaggtggc caagctgtac aacatgctgc cggccgacga ggtcggctcc
     2221 gacgggcgca ccgcggcgaa caacgccacc gtgcgctcgg tgttcatcat cggcccggac
     2281 aagaagatca agctgatgct gacctacccg atgacgaccg ggcgcaactt cgacgagatc
     2341 ctgcgcgtgc tcgactcgat gcagctgacc gccaagtaca aggtggccac gccggtgaac
     2401 tggaagcacg gcgaggacgt gatcatcacg ccggcggtca gcaacgaaga ggccgcgaaa
     2461 tccttccccg gcttcaagac catcaagccc tatctgcgca cgaccaagca gccgggctga
     2521 ggccgatccg acaccggcgg cggagatcgt catgcttgtg gttcgccagc tgttcgaccc
     2581 ccagtcgtcc acgtacacct acctgctggg cgattcggcc tccggcgagg cgctgctgat
     2641 cgatccggtg ttcgagcatg tgcgacgcga caccgccctg ctgcgcgaac tcggcctgca
     2701 cgcggtcgcc acgctggaca cgcacgtgca cgccgaccac gtcaccgggg cctggttgca
     2761 caaggtcggc agcggcagcc ggatcatggt gtccgaggcg tcggcggccg cgaacgccga
     2821 ccgtctgctc aggcacggcg accgcgtgtc gttcggcgcg cgccacgtcg aggttcgcgc
     2881 cacgccgggc cacacgaacg gctgcctgac ctacgtgctc gatgaccaga gcatggcctt
     2941 caccggcgac agcctgctga ttcgcggttg cggccggacc gacttccagc acggcagccc
     3001 agcgcggctc taccgctcgg tgtgggagca gatcctctcg cttcagcccg gctgtctgct
     3061 gtaccccggg cacgactacc gcggcctgac cgtcaccagc gtggcggagg agaaacgctt
     3121 caacccgcgc ctgggcggtg acatcgatga ggcggatttc gccggctaca tgaacaacct
     3181 gggcctgccg caccccaagc tgatggacat cgccgtgccc gccaacctgc gctgcggcca
     3241 acccgaccag accgcacccc agtcggatgg cccgtcgtgg gcgccgctga gctgcaactt
     3301 cggcggcatc tgggagatcc agccggaggc gctggaggaa tgccttgcgg gtgggcgcgg
     3361 caccgacatc caggtgatcg acgtgcgcga gccccgcgag ttcaccggcg ggctcggcca
     3421 tatccccggc gcgcgcctgg tgccgctggc cgaactcggc gcgcgcagtg ccgagttcga
     3481 caaggcgcgc ccggtggtca cggtctgccg gtcgggcgcg cgctcggcgc aggccacggt
     3541 gctgctgcag aagtccggtt tctcgcaggt agcgaatctg gccggcggca tgctgcgctg
     3601 gcgcgcggag gcgctgccgt tcaatcgagg tgcgggctga gggtctgccc cgccgccagc
     3661 gcggcggcgg tgcccgggcc ggccaggttg aagcgccgcc tcatgcgagg cgccgtgccc
     3721 gcgcctcgtc gagcgcgcgg ggcgctgaaa gctcgcgcgc cgccgctgcg acatcgccga
     3781 cgacgatgac cgccgggctg ctcaagccct cgcgcgccac ggtgtgtgcc agcgccgaca
     3841 gcgcgcagca cgcgtggcgc tgccgcgcca ggctggcatg ctgcacgatc gccaccggcg
     3901 tggccgccgg caggccctgc agcagcccgc tctcgatcgc ctcgaccgtc gcgatgccca
     3961 tgtagatcac cagcgtcagc ccttgcgccg ccgccgcggc gagcgcggcc cagtcgaggg
     4021 gtgcggcgtc gccgcgggca tggccggtca cgaagatcac gccgtgggcg tggtcgcggt
     4081 gcgtcagcgg cacgccgatc gaggtggcgg ccgccagccc cgcggtgatg ccgttgacga
     4141 cctcgacgcg cagcccgagt gcgcgcaggt gcgccgcctc ctcgccgccg cggccgaaca
     4201 cgaacgggtc gccgcccttc aggcgcacga cgcgctcgcc gttcagcgcc tcggccgcca
     4261 tcagccgctc gatgaaggcc tgcggcgtgc tcgcacagcc gccgcgcttg ccgacgtgga
     4321 tgatgcgcgc cgaacgccgc gcgtagcgca gcaccgcgtc gccgaccagg tcgtcgacca
     4381 gcagcagggt ggcctggcgg atcgcgcgca ccgccttgag cgtcagcagc tcggggtcgc
     4441 cggggccggc gccgacgagg gtgacctggc cgctcgccgc ggcgatggca tgcctgccgc
     4501 gggcggtttc ctcgatgtca ggggcgtcgc tcatccgggt gttccgactc gagggggtac
     4561 cagattgggc acccagtccc accattcgtg ctggccgtcg gcggcgttgg tctgggtgcg
     4621 atggatgtcc caggtgacca cgaagtcggg gtcgtggatg tccacgcccg agggcgcgtg
     4681 gcgatagtgc ccggggggcg ccttgaacca ggcatcgagg ttcggcggca cgatgaaatc
     4741 ggcctgcgcc acgctggcat cggcgtgcgc caggcgcacc gagatgccct gctcggcgtc
     4801 gaccttgacc accgcacccg cgtactgggt ctgcttcatc aggccgccct gcagatcgaa
     4861 gtacgacagg ccgatgacgc aggtcttgcc gagcaggtcg tcgagttgca tggccatcgt
     4921 cagtggcgca gcggggccac gccgacctgc ggcatgttgg ccaggccgat gtcgcgggca
     4981 tgttcttcga agcccttgtt cttccagaag aaggcgcccg gcatcgtggt cgggatcacc
     5041 agcacataga acagcgcgcg actgaccacg ctggtgagca gcgcggtcgc gccgaccagg
     5101 gcccagccca gccacgtgac cgggcccagg ccgccttgca gcagcatcgc cacgagcagc
     5161 ccgcaggcca gcaccccgtt cgcgccgagc agcgtgttgc gcagcatgaa ggtcttgccg
     5221 aaggtcgtgg tctggacata gtacgaggcg ccgccttcat gctcggcgcg ccccatgacg
     5281 cgggcatggg ccacgtggcc gacgccctcg atggccagcc ccagcgtgag caggccgccg
     5341 cacacccagg ccgtggcgac gacgctctcg cccgcgaggc ccagcgtggc cagcgcgacc
     5401 gcgccggtca gcagcgcgcc gagcgacagc gcactgccga cgaaggcggt ggtgacttgc
     5461 cagtggtccc agaacggccg ggccttgatg cggtagcagc ggttcatgta gtacagcccc
     5521 gccacgctgc cgagcacggc cagtgcaccg aagatcgtcg ccaggcgcgg cgccgcggcg
     5581 gcaccgggga ggcggctgac gtcgacgccg acgagcccgc tgcacagcgc ctggaaccac
     5641 gcattcgcgg gcaggctgaa caggatgtgc atgccgagcg cgccgatgaa gatggcgatg
     5701 cccaggccct cgcgcgacac cggcgaatgc cgcaggttgt tgaagccgcg gtagaagcga
     5761 tgcggcttgc ccaggtgcat ggtcgacatg aacagcccga acgccaccag catgaactcc
     5821 aggatcgcca gcggcgcgta cagcgccgac tgcgcgaacg ccacgaagcc gccgatgccg
     5881 aattgcgcac cgaagaactg cagcgcgaac gcgccggccg ctgcctgggc cgcgagcgtg
     5941 aacatcacca gcgggttctc gcgcgagctc agccgcgcga gattccagtg catcgccttg
     6001 cccttcttct ggtcgatggc cgggcggtag cggccgtgct cgtccttgtg gtacttcaag
     6061 ggcatcgagt cggtgcgcat catctcgtcg ggcagctgct tgatctgctg gaagcggatg
     6121 ttcgggcgcg tgatctcggg cgacgggaag cccgggatct cgacccgcgc ctgttcgcgg
     6181 ccttcgggca ccttctcgat cacgccgaag tcgagcgcgt tgccgacgca ggccgacacg
     6241 caggccggct tcaggccgac ctcgaggcgg tcgacgcaca tgttgcactt cgagacctgg
     6301 cccttcaccg ggtcgagctg cggcgcgttg tacgggcaca cccaggtgca gtagccgcag
     6361 ccgaagcagg tctcggggtc ttgcagcacc gcgccgtact cggcgtgctt ggtgtaggcc
     6421 cgggtcgggc aacccttcag gcagaccggg tcatcgcaat ggttgcaggc catcgagatg
     6481 ttcatgcgct tgtagtcggg gaagctgccg ccctcgacgt agccgaccga gcggaacgcc
     6541 aggtgtgccg ggttgtcgtt cttctcgctg cacgcggctt cgcaggcgtg gcagccgatg
     6601 cagttgtcgg cggtgaagaa gaagccgtgc tgcttgttgc ggttcgggtt cgtgcccacc
     6661 gccgggttct cgttgatgaa catcgagcgc ccggccggca gcttctgccc ggcgagctcg
     6721 atcaggtcga tcttctggcc gtagcggttg gcctcggcga tgttggtgtc gcgcaggaag
     6781 gcgtagctct gctcctcggt gcggatctcc cagatcttgc gcgggcgcgc tgcgctcttg
     6841 atggcttcgg acaccagcga atcgaggggc aagaggtcgt tcttcatggc agtcaccagg
     6901 caaattcaga acgcgcggcg ctcgaggttc aggcgcgccg cctcggcttg atcgaccttc
     6961 tcgacccgcg ccgcgttctg cttgaacgcg ggttgccgcg aatgcgggtc gagcaggccg
     7021 agcgtcaggc ggttcacgca gtcgtggaag tggaacggga tgaacagcat gtcgcgcccg
     7081 acgcgctgcg tgagttgcac catcacgacc gcgtcaccgc gccggctcag cacgcgcaca
     7141 tagctctggt gctcgatgcc gagctcggcc gcggcatcgg ggttcatctc catgtagggc
     7201 gtcgggctga acttgttgca gttgccgatc ttgccggtgc gcgtgcgggt gtgaaagtgc
     7261 tcgacgacgc ggcccgagtt caaccagaac gggtactcgg cgtcggggca ttcgttgttg
     7321 ttgacgaagc gcaccgggat cagcttggcg tggccgtcgg gggtctgaaa cttgccgtcg
     7381 gtgtacaggc gcgggttgcc gagaaagtct tgccggtgca gcatgtcgaa gccggcggtc
     7441 ggcccgatcg tggcggtgtc gagactggct tgcgcttcgc tgtagggcca ctggatgccg
     7501 cgtgcggctt cgatgcggtc gtaggtcatg cccgagatgt cgagcgtgcg gccctcgccc
     7561 ttggagagtt gccgcatctc gtcgaacgcg ccctcgggcg tcgcggggaa gtggatcttg
     7621 ttgcgatcct cgaagcgctt cgccatctcg ttgaagatcc agaagtcggg cttgctgtcg
     7681 gcgtagggcg gcaacacgtt gcgcgtgagg ttgacgcggc gctcggtgtt ggtgtggcag
     7741 ccttccttct cggcccacac ggcggcgggc agaaatacgt gcgagtactt gttggtctcg
     7801 acgtcttcgt acacgtcctg cacgaccagg aattcgaggt tctccatcgc cttgacgatg
     7861 cgtggctggt tcgccatcga ggtcatcgga ttcgtcgcga ccagccacag gcccttgatc
     7921 tcgccggtct cgatggcggg gaagatgtcg gtctgcgcca ggccgcgcgc ggcggggaag
     7981 aagctttcgt cgatgcccca gaaatcggcg accaccttgc ggtctttctc tttctcgagc
     8041 aggcggtagc cgggcaggcc cgagcacgac gaccactcgc gcgtgcccat cgcattgcac
     8101 tggccggtga tcgacaggct ggtgccgccg gggatgccga tgttgccggt gatgaggttc
     8161 aggttgttga tgttgacgac gccgtcggag ccgtgcgtgg actggttgat gcccatggtc
     8221 cagatgctca tcgccgccgg tgccttggcg tagatgcgcg ccacggtgcg gatgcggtct
     8281 tcgtcgatgc cgcagacgtg ctgcgcggtc ttcgggtcgt agtcggccag cagcgcgcgc
     8341 aggccgtcga tgccttgggt gtggttggcg atgtaggcgt cgtcctgcag gccctctttg
     8401 aagatcacat gcatcatcgc gttgatgagc acggtgtcgg tgccgggcgt gatgggcagg
     8461 tggatgtcgg cgaactgcgc gagcatggtg acgcgcgggt cgaccacgat gagcgggaac
     8521 ttgcgcttct ccagcgcttc cttgaggcgc cagtagatga tggggtgctg ctcgggcagg
     8581 ttcgagccga aagccatcag gcagtgcgtg tgctcgaaat cgccatagca gccgggcggg
     8641 ccgtcggagc cgaacgagcg cttgtagccc gacaccgccg aggccatgca cagcgtggtg
     8701 ttgccgtcgt agctgttggt gccgatctgg ccccgcacca gcttgcccag cgtgtagaac
     8761 tcttcggtga gcaactggcc ggtggagacc acggcgaacg aatcgcggcc gtacttggcc
     8821 tggatttcct tgatcttcgc cgcggtctgg tcgagcgccg catcccagct cgcgggctgg
     8881 aacgcctggt gcgcccggtc gcgcatcaac ggcacccggc cgcgccccgg cgaggtgaac
     8941 agctcgtgtt ccagcaggcc cttgatgcac agcttgccgc ggttcacgtc ggcgccggcg
     9001 tggccgcggc tggtgacgat ctggccgctt tcgttcaggc cgatttcaat ggcacaaccg
     9061 gtcgagcagt agttgcaggt cgtgtacttc cactccttca cctccttgac ggggatggcg
     9121 atgggcttgc gtcgagtgcg gccgaacagc atcgtcgggt cccggtggtt caggcggtgg
     9181 tgcgggcgcg cggcccggcc accggggcga cggcgtcgag tgcccgcgtg gccagctcga
     9241 cgctgtcgag gaagacctcg ccgccttcga ccttgcaggc gaagctgacg gtgcaaccct
     9301 cgtcgggcgc cttggcgcag ccatcggcca ggccgatggt ccagttgtgc agcgggcagg
     9361 ccacgctctc gccgaacacg atgccctggc tcagcggccc gcccttgtgc gggcagcggt
     9421 cgagcagcgc gaagaccttg tcctcggcgt tgcggaacac ggccacggcg atggcgtcgg
     9481 cacgggcgac gcgccgggca ccgagcaccg gaatgtcggt gagggtgcag atgcttttcc
     9541 agttgctcat ggggttgtca gctcagttgg ggcacgccgc ttcacggcac cgccaccgga
     9601 ttgaattgcc gcgtgtccac cgtcgccttg tcgaactcga accacgggtc gggctcgcct
     9661 tcgagcgcgg cttgcagatt cgcccacagt gccttgcggc cggccgcgtc gtcgaggatg
     9721 cgcgccttca cgtagtcgag gccgacgcgg ctcacgtagt gcaccgtgcg ctccaggtac
     9781 cagccttcct ggcggtacag ctcgcagaac gcgccggtgt attcgagcac ttcggcggcg
     9841 gtcttgagct tgacgaagaa gtgtgcgacc tcggtcttga tgccgccgtt gccggccacg
     9901 tacatctccc agcccgaatc gacgccgatg atgcccacgt ccttgatgcc ggcctcggcg
     9961 cagttgcgcg ggcagccgct gaccgcgaac ttgaccttgt gcggggcgta catgcgccac
    10021 atcgcgcgct ccagatcctt gcccatctgg gtgctgtcct gcgtgcccat gcggcaccac
    10081 tcggagccga cgcatgtctt cacggtgcgc agcgccttcg cataggcgtg gcccgagggc
    10141 atgccgatgt ctttccagac gttgaccagg tcttccttct tcacgcccag caggtcgatg
    10201 cgctgcccgc cggtgacctt gacggtgggg atcttgtact tgtcgaccgc atcggcgata
    10261 cgccgcaact cgctggcggt ggtctcgccg ccccacatgc gcgggatcac cgagtaggtg
    10321 ccgtccttct ggatgttggc gtggctgcgc tcgttgatga agcgcgattg cgggtcgtcg
    10381 cgggcctcct tgggccaggt gctgatcagg tagtagttga cggccgggcg gcagctggag
    10441 cagccgttcg gggtgcgcca gcccatggcc ttgtagaccc cgtcgatgct gagcaggcgg
    10501 tcgttgcgga tcgactcgcg cacgtcctgg tggctcgcat cggtgcaagc gcacatcggc
    10561 ttcttggtcg gcgccttcga gtagtcgccg ccggccgtga acatcagcaa ctgctcgacc
    10621 agaccggtgc acgaaccgca gctcgcgctc gccttcgtgt gcttcttgac ctcttcgagc
    10681 gtgaacaggc ccttctcctt gatggccttg cagatcgtgc ccttgttgac gccgttgcag
    10741 ccgcagacct cgtcggcgtc ggccatcgcg gccgccttgc tgtggccctc gtggccggtg
    10801 tcgccgatgt tggattcgcc aaacatgagc cggtcgcgga tgtccgagac cttgcggccg
    10861 tcgcgcagca gcttgaagta ccaggagcca tccaccgtgt cgccatacag gcaggcgccg
    10921 atgagcttgt cgtccttgat gaccagcttc ttgtagac
//
LOCUS       DATMJB010000436         2788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_826111, whole genome shotgun sequence.
ACCESSION   DATMJB010000436 DATMJB010000000
VERSION     DATMJB010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826111"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VNU48_09405"
     CDS             complement(<1..609)
                     /locus_tag="VNU48_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HWI11532.1"
                     /translation="MDNDKELIRRIRADLADSFDEELELELDDRAVDDVSGSSSEPDD
                     DHKEVRRRYFRELFRLQGELVKLQDWVVAKRHKVVIIFEGRDAAGKGGVIKRITQRLN
                     PRVARVAALPAPNDRERTQWYFQRYVAHLPAAGEMVLFDRSWYNRAGVERVMGFCDDA
                     QYEEFFRSVPEFEKMLVRSGIQLIKYWFSISDEEQHLRFLGRI"
     gene            complement(729..1412)
                     /locus_tag="VNU48_09410"
     CDS             complement(729..1412)
                     /locus_tag="VNU48_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HWI11533.1"
                     /translation="MASPAVTPLRIQIREGESVSALLCAPPDPRACYVFAHGAGAGMN
                     HDFMAAIASGLAERGVATLRYQFGYMERGSKRPDVPMVAHLAVRAAVAEAARLLPALP
                     LFAGGKSFGGRMTSQAQAERPLAGVRGLAFVGFPLHPAGKPSIERATHLREVLCPMLF
                     LQGTRDELADLAQLREALTPLGDRATLELLADADHSYHVRARSGRSDDQVRASLVDAM
                     TRWFADHAG"
     gene            complement(1431..2240)
                     /locus_tag="VNU48_09415"
     CDS             complement(1431..2240)
                     /locus_tag="VNU48_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI11534.1"
                     /translation="MRGIEEMLGLGRSVITGLIDAGFVSPARGARNEYRFTFQDVVLL
                     RTAYHLRAAKIPARKMLSSLRQLKERLPQELPLSGLRIRAIGNDVAVRTAGAGWEAQS
                     GQLLMDFEVASSSQGSVAFLQRSSAAPAAQPTTAAEWLARGEACEGENAAAAEAAYRR
                     SIAIEPDFVAAYLNLGALLCETGQSAQALALYDKAIVRGAGSPLLHFNRAIALEDLHR
                     QTEALTSYEHCLALAPDFADAHYNAARLHEMLGHAQAAVKHYSAYRRLQLG"
     gene            complement(2270..>2788)
                     /locus_tag="VNU48_09420"
     CDS             complement(2270..>2788)
                     /locus_tag="VNU48_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWI11535.1"
                     /translation="KSGRCALARWSWKSKQYVVQVRAVADGLILQQLLYAEEVRSLKD
                     LDIEKVDVSAAELTLALQLIDQISEDAYDPGAFHDDEKKRILAAIDEKISGKQVVASA
                     HAEAPTSGQVIDLMDALRASLGKQRGKAPATAAESAEAIPEGALKERKPARRAPKVAE
                     PEAAPARTRARK"
BASE COUNT          425 a          942 c          965 g          456 t
ORIGIN      
        1 gatgcggccg aggaagcgca ggtgctgctc ctcgtcggag atcgagaacc agtacttgat
       61 cagctggatg ccggagcgca ccagcatctt ctcgaactcg ggcacggagc ggaagaattc
      121 ctcgtactgg gcgtcatcgc agaagcccat cacgcgctcg acgccggcgc ggttgtacca
      181 gctgcggtcg aacagcacca tctccccggc ggcgggcaga tgcgccacgt agcgctggaa
      241 ataccactgc gtgcgctcgc ggtcgttcgg cgccggcagc gcggcgacgc gcgcgacccg
      301 cgggttcagg cgctgcgtga tgcgcttgat cacgccacct ttgccggccg cgtcgcggcc
      361 ctcgaagatg atgacgacct tgtggcgttt ggcgacgacc cagtcctgca gcttgacgag
      421 ctcgccctgc agccggaaca gctcgcgaaa gtagcggcgg cggacttcct tgtggtcgtc
      481 gtcaggctcg ctcgagctgc ccgacacgtc gtcgacggca cgatcgtcga gctcgagctc
      541 gagttcctcg tcgaagctgt cggcgaggtc ggcacggatg cgccggatca gctctttgtc
      601 gttgtcgatg ttcaaacggt tccccggtgg cgcggtctgg cgagagacca cgctacgggg
      661 cacgtgtggc aaacgtgtga catggcgacg ccatgccgaa catccctacg atgggccgcc
      721 tttcggcatc acccggcgtg atcggcgaac caacgcgtca tcgcatcgac cagcgaggct
      781 cgaacctggt cgtcgctgcg cccgcttcgc gcacgcacgt ggtacgagtg gtcggcatcg
      841 gccaagagtt cgagcgtggc acgatcgccc agcggcgtga gcgcttctcg cagctgggcc
      901 aggtcggcga gctcgtcgcg cgttccctgc agaaacagca tcgggcagag cacctcgcgc
      961 agatgcgtcg cacgctcgat cgacggcttg cccgcggggt gaagagggaa gccgacgaac
     1021 gcgagcccgc gcacgccggc gagcggccgt tcggcctggg cctgcgaggt catgcgcccg
     1081 ccgaacgact ttccgccagc gaacaggggc agcgccggga gcagccgagc ggcctcggcg
     1141 accgcggctc gaaccgcaag gtgggcgacc atcggaacgt ccgggcgctt cgagccgcgc
     1201 tccatgtagc cgaactgata gcgcaaggtg gcgacgccgc gctcggccag cccgctggcg
     1261 atggccgcca tgaagtcgtg gttcatgccg gcgccggcgc catgcgcgaa cacatagcag
     1321 gcccgcggat ccggcggcgc gcaaagcagc gcggacaccg attcaccttc ccgaatttgg
     1381 attcgcaacg gcgtgacggc aggcgaggcc atgtgcgcgg ctcaggtcgc ctaccccagc
     1441 tgcaggcgtc ggtaggcgct gtagtgtttc accgcggcct gggcgtgccc gagcatttcg
     1501 tgcagccggg cggcgttgta gtgcgcatcc gcgaagtcgg gcgcgagcgc gaggcaatgt
     1561 tcgtagctcg tgagcgcttc ggtctgccga tgcagatctt cgagcgcgat ggcgcgattg
     1621 aagtgcagca agggcgaacc cgcgccgcgc acgatggcct tgtcgtacag cgcgagtgcc
     1681 tgggcacttt gcccggtctc gcaaagcagc gcgccgaggt tcaggtaggc ggcgacgaag
     1741 tcgggctcga tcgcgatgct gcgtcgatag gcggcttcgg ccgcggccgc gttttcgcct
     1801 tcgcaggcct cgccgcgcgc cagccattcg gcggctgtcg tgggctgcgc cgccggcgca
     1861 gcggagctgc gctgcaggaa cgccacgctg ccctgcgaag acgacgcgac ctcgaagtcc
     1921 atcagcaact gaccgctctg tgcctcccac ccggcgccgg cggtgcgcac cgccacatcg
     1981 ttgccgatcg cgcggatccg caggccgctc agcggcagtt cctgcggcag ccgctccttg
     2041 agctggcgca gcgagctcag catcttgcgg gccgggatct tcgcggcgcg caggtgatag
     2101 gccgtgcgca gcagcacgac gtcctggaac gtgaaccggt attcgttgcg ggcgccgcgc
     2161 gccggcgaca cgaagccggc gtcgatcagg ccggtgatga cgctgcgccc gaggcccagc
     2221 atctcttcga tgccccgcat ggtgtacgga gcgccggagg cggcacgcgt cacttgcggg
     2281 ctcgggtgcg cgcgggtgcg gcctcgggtt cggccacctt cggggcacgc cgggccggct
     2341 tgcgttcctt caacgcacct tcggggatcg cctcggcgct ctcggcagcc gtcgccggcg
     2401 ccttgccgcg ctgcttgccg agactggcac gcagtgcatc catcaggtcg atgacctggc
     2461 cgctggtcgg cgcttccgcg tgggcggacg cgaccacctg tttgccggag atcttctcgt
     2521 cgatcgccgc caggatgcgc ttcttctcgt cgtcgtggaa cgcgccgggg tcgtaggcgt
     2581 cttcggagat ctgatcgatc agctgcagcg ccagggtcaa ttcggccgcg gacacatcga
     2641 ccttctcgat gtccagatcc ttcagcgagc gaacctcttc cgcatacagc agctgctgca
     2701 ggatcaggcc atcggcgacc gcacgaacct gcacgacgta ttgtttggat ttccagctcc
     2761 agcgcgccag cgcacagcgg ccgctctt
//
LOCUS       DATMJB010000437         2037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_435139, whole genome shotgun sequence.
ACCESSION   DATMJB010000437 DATMJB010000000
VERSION     DATMJB010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435139"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VNU48_09425"
     CDS             complement(<1..198)
                     /locus_tag="VNU48_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11536.1"
                     /translation="MKPDDVDDRAAGRAPGRSMGHEEAHFVDLAGPLPPGGPADVFVG
                     RGAAMNAPYRPPPALLDARRKG"
     gene            complement(195..1019)
                     /locus_tag="VNU48_09430"
     CDS             complement(195..1019)
                     /locus_tag="VNU48_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI11537.1"
                     /translation="MNTARPSRDPAWRARVFWFGAALVLLWPMLVATEFRPWVLFGAE
                     SRGPTLRFLAAFFPPELGGEFLWLVARETWRTVAIATAGITLALLIAIPATLMATRVL
                     SVSALSGRMAPLPAALRQGLRWALVLLRSVPELVWALVFVRVVGLGPTAGVLAIALTY
                     GGMLGKVYADILESGETQATRNLLANGSGRLQAFAYGLLPQNAAELTSYTVYRWECAI
                     RSSVVLGFVGAGGLGQQLDNSTKMFAGGEVATMLLVFVALVALADRASAWLRRALA"
     gene            complement(1016..1846)
                     /locus_tag="VNU48_09435"
     CDS             complement(1016..1846)
                     /locus_tag="VNU48_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI11538.1"
                     /translation="MDIDLQTVHARHPAAARGTTALRGVGLSLRAGERLAVIGPSGAG
                     KTTLLQVAAAALKPSSGRVQLAGIDPWSQSTRALQRRRGRLFMAPQTPPLPPRQRVVT
                     AVLAGRLPAWSLATSLRSLFYPADIAGAHAALERFDLADKLFARVDRLSGGERQRVGL
                     ARALLAPATLWLIDEPLSALDPTRAEQAIAALIDVARERGITLLATMHQVDTALAHFD
                     RIVGLRDGAVAFDLPTPQVSRDHLLRLYAQHEDELDGAPRAPASPPSATPRPVEMHCR
                     "
     gene            complement(1871..>2037)
                     /locus_tag="VNU48_09440"
     CDS             complement(1871..>2037)
                     /locus_tag="VNU48_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative selenate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWI11539.1"
                     /translation="EALRTKLTQAFLTLDRATPEGKEILELQRATRFVPTRPENYKGI
                     EAAAKSAGLL"
BASE COUNT          286 a          755 c          753 g          243 t
ORIGIN      
        1 gcccttgcgg cgcgcgtcca acagcgccgg cggcggccgg tacggcgcgt tcatggcagc
       61 ccctcggccg acgaatacgt cggccggtcc cccgggggga agcgggccgg cgaggtccac
      121 aaagtgggcc tcttcgtggc ccatggagcg gcccggcgct cggcccgcgg cgcggtcgtc
      181 gacatcgtca ggcttcatgc cagcgcccgc cgcaaccacg cgctggcgcg atcggccagc
      241 gccaccagcg cgacgaacac gagcagcatc gtcgcgacct cgccgcccgc gaacatcttg
      301 gtggagttgt cgagctgctg cccgagcccg cccgcgccga cgaagccgag caccaccgac
      361 gagcggatcg cgcactccca gcggtacacc gtgtacgagg tgagctcggc cgcgttctgc
      421 ggcaacagcc cgtacgcgaa cgcctgcagg cggccgctgc cgttggccag caggttgcgc
      481 gtggcctgcg tctcgccgct ttcgaggatg tcggcgtaga ccttgccgag catgcccccg
      541 taggtcagcg cgatcgcgag cacgccggcc gtcggcccga ggccgacgac gcgcacgaac
      601 accagcgccc acaccagttc gggcacgctg cgcaacagca ccagcgccca gcgcagcccc
      661 tggcgcagcg cggccggcag cggcgccatg cgcccggaca gcgccgagac cgacagcacg
      721 cgcgtggcca tcagcgtggc cggaatcgcg atcagcagcg ccagcgtgat gccggcggtg
      781 gcgatcgcca cggtgcgcca ggtctcgcgt gcgaccagcc acaggaactc gccgccgagc
      841 tccggcggga agaacgcagc gagaaaccgc agcgtcggcc cgcggctctc ggcaccgaac
      901 agcacccacg gccggaactc ggtcgcgacc agcatcggcc acagcagcac cagcgccgcg
      961 ccgaaccaga acacgcgggc ccgccaggcc ggatcgcggc tcgggcgcgc ggtgttcatc
     1021 ggcagtgcat ctccacgggt cgtggcgtgg cactcggcgg gctggcgggc gcgcgcggcg
     1081 cgccatcgag ttcgtcctcg tgctgcgcgt aaagccgcag caggtgatcg cggctgacct
     1141 gcggcgtggg cagatcgaac gccaccgcac cgtcgcgcag gccgacgata cgatcgaagt
     1201 gcgcgagcgc ggtgtcgacc tggtgcatcg tcgccagcag ggtgatgccg cgctcgcgcg
     1261 ccacgtcgat cagcgcggcg atggcctgtt cggcgcgcgt cgggtcgagc gccgacaagg
     1321 gttcgtcgat cagccacagc gtggccggtg cgagcagcgc gcgcgccagc ccgacgcgct
     1381 ggcgctcgcc gcccgacagg cgatcgacgc gcgcgaacag cttgtcggcc aggtcgaagc
     1441 gctcgagcgc ggcgtgcgcc ccggcgatgt ccgccgggta gaacagcgag cgcaggctgg
     1501 tcgcgaggct ccacgccggc agccggccgg ccagcaccgc ggtgacgacg cgctggcgcg
     1561 gcggcagcgg cggtgtctgc ggcgccatga acaggcgccc gcgccggcgt tgcagcgcgc
     1621 gcgtgctctg cgaccacggg tcgatgccgg ccagttgcac acgaccgctc gacggcttca
     1681 gcgcggcggc cgcgacctgc agcagcgtgg tcttgccggc gcccgagggc ccgatcacgg
     1741 ccagccgctc gcccgcgcgc aggctcaggc cgacgccgcg cagcgcggtc gtgccgcgcg
     1801 cggccgccgg gtggcgcgcg tgcacggttt gcaggtcgat gtccacgggc ggcgggtcgc
     1861 gatccgatgc tcagagcagg ccggcgctct tcgcggcggc ctcgatgccc ttgtagttct
     1921 cgggccgcgt cggcacgaag cgggtcgcgc gttgcagctc gaggatctcc ttgccttcgg
     1981 gcgttgcgcg gtccagcgtc aggaaggcct gggtcagctt cgtgcgcagc gcttcgg
//
LOCUS       DATMJB010000438         2507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_776392, whole genome shotgun sequence.
ACCESSION   DATMJB010000438 DATMJB010000000
VERSION     DATMJB010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776392"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="VNU48_09445"
     CDS             <1..1683
                     /locus_tag="VNU48_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWI11540.1"
                     /translation="GLLITDQQQRVLEANPTFTEITGYARDELLGLVPAMLQATEAEV
                     ALNTQLAAMRAGLVNEGTWRGELQQHRRNGEPCLLQVTITAVRDANGSVRNHVLAIAD
                     ITHTRRQLEQLQRQAHFDELTRLPNRVRLAQMLQTAMQASRREGSLLTVCYLDLDHFK
                     PVNDRFGHEAGDRLLVELANRMRRSLRSWAGGNDVVARIGGDEFVLLLRTATLEESRH
                     AVERVLNQVGQPYGLGVGSGPVLVTASVGATVFPLDGADAETLLRHADHAMYGAKQAG
                     RNGYLFFDAEHDRRAEARFVALGRVQEALDANEFRLYYQPKVDMRNAKVLGVEALLRW
                     KHPDQGVISPAQFLPLIENTGLAISVGNWVLQQGIDQLARWLRLGLDITVSINISARH
                     LQEPLFAQHLAGLLSRHQAPVAHRLVIEVLETAALADIDHTCELMEQCRTLGVRFALD
                     DFGTGYSTFTYLKQLPIDMLKIDRSFVTNMLADRQDLAIVEGVIGLSQTFGCSVVAEG
                     VETRPQAQRLIEIGCSIGQGNGIAAAMPAEHVVDWVRAYKGMPSASELFAPA"
     gene            1745..>2507
                     /gene="dnaG"
                     /locus_tag="VNU48_09450"
     CDS             1745..>2507
                     /gene="dnaG"
                     /locus_tag="VNU48_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HWI11541.1"
                     /translation="MIPAAFIQDLLARVDIVEVVGRHVQLKKGGANLMGLCPFHGEKS
                     PSFSVSPSKQFYHCFGCGASGDAIRFLTEYSGMSFLDAVRELAQQVGLQVPEEDSSPA
                     EREQAAAQKQRRSTLTEVLAKAAEAYRKQLRQSPRAVDYLKGRGLSGEIAAAFGLGYA
                     PDGWRNLASVFPSYDDPLLEESGMVIAKDAEDGSTDEADRKRYDRFRDRIMFPIRSVQ
                     GEVIGFGGRVLDKGEPKYLNSPETPVFVKGRELYGL"
BASE COUNT          403 a          864 c          877 g          363 t
ORIGIN      
        1 ggcctgctga tcaccgatca gcagcagcgc gtgctggagg cgaacccgac cttcaccgag
       61 atcaccggct acgcgcgcga cgagctgctc ggtctggtgc cggcgatgct gcaagccacc
      121 gaggccgagg tcgcgctgaa cacccagctc gcggcgatgc gcgccggcct cgtcaacgag
      181 ggcacctggc gcggcgaact gcagcagcat cgccgcaacg gcgagccctg cctgctgcag
      241 gtgacgatca ccgcggtgcg cgacgcgaac ggttcggtgc gcaaccacgt gctggccatc
      301 gccgacatca cgcacacgcg ccggcagctc gagcagctgc agcgccaggc gcacttcgac
      361 gagctgacgc gcctgccgaa ccgggtgcgc ctcgcgcaga tgttgcagac ggcgatgcag
      421 gccagccgcc gcgaaggctc gctgctgacc gtgtgctacc tcgacctcga tcacttcaag
      481 cccgtcaacg atcgcttcgg gcacgaggcc ggcgatcgcc tgctggtcga gctggcgaac
      541 cgcatgcgcc gctcgctgcg ctcgtgggcc ggcggcaacg acgtggtcgc gcgcatcggc
      601 ggcgacgagt tcgtgctgct gctgcgcacc gccacgctgg aggagagccg tcatgcggtc
      661 gagcgcgtgc tcaaccaggt cggccagccg tacgggctgg gcgtcgggag cggcccggtg
      721 ctggtcacgg cgagcgtcgg cgcgaccgtg ttcccgctcg acggcgccga cgccgaaacg
      781 ctgctgcgcc acgccgacca cgcgatgtac ggcgccaagc aggccgggcg caacggctac
      841 ctgttcttcg acgccgagca cgatcgccgg gccgaggcgc gcttcgtcgc gctcgggcgc
      901 gtccaggagg cgctcgacgc caacgagttc cgcctgtact accagcccaa ggtcgacatg
      961 cgcaacgcca aggtgctcgg cgtcgaggcg ctgctgcgct ggaagcaccc ggaccagggc
     1021 gtgatctcgc cggcgcagtt cctgccgctg atcgagaaca cggggctggc gatcagcgtc
     1081 ggcaactggg tgctgcagca gggcatcgac cagctcgcgc gctggctgcg gctcgggctg
     1141 gacatcacgg tcagcatcaa catctcggcg cgtcacctgc aggagccgct gttcgcccag
     1201 catctggccg ggctgttgtc gcggcatcag gcgccggtcg cgcaccggtt ggtcatcgag
     1261 gtgctcgaga ccgcggcgct ggccgacatc gaccacacct gcgagctgat ggaacagtgc
     1321 cgcacgctgg gcgtgcgttt cgcgctcgac gacttcggca ccggttactc caccttcacc
     1381 tacctgaagc agctgccgat cgacatgctg aagatcgacc gctcgttcgt caccaacatg
     1441 ctcgccgacc gccaggacct ggcgatcgtc gagggcgtga tcggcctgtc gcagaccttc
     1501 ggctgcagcg tcgtcgccga aggtgtggaa acgcgcccgc aggcgcagcg gctgatcgag
     1561 atcggctgca gcatcggtca gggcaacggt atcgccgcgg ccatgccggc cgagcacgtc
     1621 gtcgactggg tgcgcgcgta caagggcatg cccagcgcca gcgagctgtt tgcgccggcc
     1681 tgacggcggc cggccgggcc cggcgccggc cgcgccgcgc aatgcgcgac gctagactcg
     1741 acaggtgatc ccagccgcct tcatccagga cctgctcgcg cgcgtcgaca tcgtcgaggt
     1801 ggtgggccgc cacgtgcagc tgaagaaggg cggcgcgaac ctgatgggcc tgtgcccgtt
     1861 ccacggcgag aagtcgccga gcttctcggt cagcccgagc aagcagttct accactgctt
     1921 cggttgcggc gcgagcggcg atgcgatccg cttcctgacc gaatacagcg gcatgagctt
     1981 tctcgatgcg gtgcgggaac tggcgcagca ggtcggcctg caagtgcccg aggaagacag
     2041 ttcgcctgcg gaacgcgagc aggccgcggc gcagaagcag cgccgctcga ccttgaccga
     2101 ggtcctggcc aaggcggccg aggcctaccg caagcagctc aggcagagcc cgcgcgcggt
     2161 cgactacctg aagggccgcg ggctgagcgg cgagatcgct gcggccttcg gcctcggcta
     2221 tgcccctgac ggctggcgca acctggcgag cgtctttccg agctacgacg atccgctgct
     2281 cgaggagagc ggcatggtga tcgcgaaaga tgccgaggac ggctcgaccg acgaggccga
     2341 ccgcaagcgc tacgaccgct tccgcgatcg catcatgttc ccgatccgct cggtacaggg
     2401 cgaggtgatc ggcttcggcg gcagggtgct cgacaagggc gaaccgaagt atctgaactc
     2461 tcccgagacg ccggtgttcg tgaaggggcg cgagctgtac ggccttc
//
LOCUS       DATMJB010000439         2038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_302241, whole genome shotgun sequence.
ACCESSION   DATMJB010000439 DATMJB010000000
VERSION     DATMJB010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302241"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="VNU48_09455"
     CDS             <1..1448
                     /locus_tag="VNU48_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWI11542.1"
                     /translation="AEKAMIPMLDILQSVPILGFQAIAIAPFIALFPGNIFGVECAAI
                     FAIFTSQAWNMAFSLYQSLKTVPAELREAAAVFQLSGWQRFWRLELPFAMPGLLWNMM
                     MSMSGGWFFVVASEAISVSNQSIKLPGVGSYIALAIEQRDLGAIGWAIGGMLVGIVLY
                     DQLFFRPLLAWADKFRFEEGTSDAAPTSWMLTWMRRTDALSALALRFVRLSERSLLMF
                     RRQHDGTSIRARTTPVNPVWSRVWDAAVAAAVLLAVWQLVAFIHAEVGFGEVLHVFVL
                     GLYTMLRVLVLIALAALVWVPVGIWIGMNPLWAGRLQAVAQFLAAFPVNLMFPLVVVL
                     IVRWHLNPNLWLSPLMVLGTQWYLLFNVIAGASTVPTELRYAAHNLGLTGWLKWRRYL
                     LPAVFPSFVTGAITASGGSWNASIVAEYVTWGDTTLQAQGLGSYIAHMTAIGDFPRIA
                     LGIGVMCVFVMSMNHFVWRRLYRMAEDRMHF"
     gene            1470..>2038
                     /locus_tag="VNU48_09460"
     CDS             1470..>2038
                     /locus_tag="VNU48_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI11543.1"
                     /translation="MAQAIVELKGVGKSFRSADGHLRSVLEGVDFRLAEGEIVSLLGQ
                     SGSGKSTMLRIMAGLIPADAGEVRLRDQPLYGPARGVSMVFQSFALFPWLTVQANVEL
                     GLEARGVASAERERRAEAAIRLIGLTGFEGALPRELSGGMRQRVGIARALVVEPEVLL
                     MDEAFSALDVLTGERLREDILELWASKAMP"
BASE COUNT          265 a          668 c          727 g          378 t
ORIGIN      
        1 cggccgaaaa ggcgatgatc ccgatgctgg acatcctgca atcggttcct attctcggct
       61 ttcaggcgat cgcgattgcg ccgttcatcg cgctattccc cggcaatatc ttcggcgtcg
      121 agtgcgcggc gatcttcgcg atcttcacct cgcaggcctg gaacatggcg ttcagcctgt
      181 accagtcgct gaagaccgtg ccggccgaac tgcgcgaggc ggccgcagtg ttccagctct
      241 cgggctggca gcgcttctgg cgcctggagc tgccgttcgc gatgccgggg ctgctgtgga
      301 acatgatgat gtcgatgtcg ggcggctggt tcttcgtcgt cgcgtcggag gcgatctcgg
      361 tgtcgaacca gagcatcaag ctgcccggcg tgggctcgta catcgcgctg gcgatcgagc
      421 agcgcgacct cggcgcgatc ggctgggcga tcggcggcat gctggtgggc atcgtgctgt
      481 acgaccagct gttcttccgc ccgctgctgg cctgggccga caagttccgc ttcgaggaag
      541 gcacgagcga cgccgcgccg acctcgtgga tgctgacctg gatgcgccgc accgacgcgc
      601 tgagcgcgct cgcgctgcgc ttcgtgcgcc tgtccgagcg ctcgctgctg atgtttcgcc
      661 gccagcacga cggcacttcg atccgcgccc gcacgacccc cgtgaacccg gtctggtcgc
      721 gcgtctggga cgcagccgtc gcggccgcgg tgctgctcgc ggtctggcag ctcgtcgcct
      781 tcatccacgc cgaggtcggc ttcggcgagg tgctgcacgt gttcgtgctc ggcctgtaca
      841 cgatgctgcg cgtgctggtg ctgatcgcgc tggcggcgct ggtgtgggtg ccggtgggca
      901 tctggatcgg catgaatccg ctgtgggccg ggcggctgca ggccgtggcg cagttcctcg
      961 ccgcattccc tgtgaacctg atgttcccgc tggtggtggt gctcatcgtc cggtggcact
     1021 tgaacccgaa cctgtggctg tcgccgctga tggtgctggg cacgcagtgg tacctgctgt
     1081 tcaacgtgat cgcgggcgcc tcgaccgtgc cgaccgaact gcgctacgcc gcccacaacc
     1141 tgggcctgac cggctggctg aagtggcgcc gctacctgct gcccgcggtg ttcccgagct
     1201 tcgtgaccgg tgcgatcacc gccagcggcg gctcgtggaa cgccagcata gtcgccgaat
     1261 acgtgacctg gggcgacacc accttgcagg cgcagggcct gggcagctac atcgcacaca
     1321 tgacggcgat cggcgacttc ccgcgcatcg cgctgggcat cggcgtgatg tgcgtgttcg
     1381 tgatgagcat gaaccacttc gtctggcggc gcctgtaccg catggccgaa gaccgcatgc
     1441 acttttgaat tgaatcgaga ggtggagtca tggcccaggc catcgtcgaa ctgaaggggg
     1501 tcggcaagtc gttccgctcg gccgacgggc atttgcgctc ggtgctcgaa ggcgtggact
     1561 tccgcctcgc cgagggcgag atcgtctcgc tgctcggcca gagcggctcg ggcaagtcga
     1621 cgatgctgcg catcatggcc gggctgatcc cggccgacgc cggcgaggtg cggctgcgcg
     1681 accagccgct gtacgggccg gcgcgcggcg tcagcatggt gttccagtcg ttcgcgctgt
     1741 tcccgtggct gacggtgcag gccaacgtcg agctcggcct ggaggcgcgc ggggtcgcgt
     1801 cggccgagcg cgaacggcgc gccgaagcgg cgatccggct gatcggcttg accggcttcg
     1861 agggcgcgct gccgcgcgag ctctcgggcg gcatgcgcca gcgcgtgggc atcgcgcgcg
     1921 cgctcgtcgt cgagcccgag gtgctgctga tggacgaggc cttctcggcc ctggacgtgc
     1981 tcaccggcga gcgcctgcgc gaggacatcc tcgagctgtg ggccagcaag gcgatgcc
//
LOCUS       DATMJB010000440         2488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_791675, whole genome shotgun sequence.
ACCESSION   DATMJB010000440 DATMJB010000000
VERSION     DATMJB010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791675"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /gene="aceK"
                     /locus_tag="VNU48_09465"
     CDS             <1..896
                     /gene="aceK"
                     /locus_tag="VNU48_09465"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HWI11544.1"
                     /translation="LYNALGLAKQGKNAFYRDFLYHLRNSSDNFRIAPGIKGMVMLVF
                     DLPSFPYVFKVIKDFFPEQKDTTRELIKRKYLIVKQHDRVGRMADTLEYSNVAFPRER
                     FEDELVAELKHFCPSLLEEDADALVIRHVYIERRMIPLNIYLQEASLAQIEAAVIEYG
                     NAIKDLLRANIFPGDMLWKNFGVTRHGKVVFYDYDEIEYLTECNFRKVPTPRNEEEEM
                     SAEVWYKVGPKDVFPETFGPFLLGNPGVRQVFMKHHAELLEASFWQGHKERILAGYVH
                     DVFPYDAHKRFQHRRTRVAAT"
     gene            924..2117
                     /locus_tag="VNU48_09470"
     CDS             924..2117
                     /locus_tag="VNU48_09470"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWI11545.1"
                     /translation="MAESIVIVSAARTPIGGLLGDFSSLSAHQLGSIAIKAAVERAGI
                     TGDAVDEALMGNCLMAGQGQAPARQAVLGAGLPQSVGAVTLSKMCGSGMRALMFAHDM
                     LSSETANVMIAGGMESMTNAPHLTPMRKGVKYGMAALYDHMAIDGLEDAYERLPNGAG
                     KAMGVFAEDCVSKYKFSREQMDQFAVDSTRRSKQANEDGSFDWEMAPVTLSGPKGDTV
                     IKFDEQPFKAKLDKIAGLKPAFRKDGTITAATSSSISDGAAALVLMRESTASSLGCTP
                     LARIRGHAVHAQAPNWFTTAPAGAIEKLLKRSGWAAKDVDLWEVNEAFAAVTLAAMTE
                     FKLSHEIVNVHGGAVALGHPIGASGARIVVTLLGALRKRGLKKGVAALCIGGGEATAL
                     ALELI"
     gene            2118..>2488
                     /locus_tag="VNU48_09475"
     CDS             2118..>2488
                     /locus_tag="VNU48_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWI11546.1"
                     /translation="MDKPLDPRLIAVGEKVTRRMRYSRADIAQFARMSLDENPLHLDS
                     MVAQRARFGEIVASGQQTAAILMGMLATHYSRSDDGVARQMICLNMNFAFKAPVFAEQ
                     EIVLQWKVSSVTPNAKLKGLVG"
BASE COUNT          456 a          830 c          829 g          373 t
ORIGIN      
        1 agctatacaa cgcgctcggg ttagcgaagc agggcaagaa cgcgttctac cgcgacttcc
       61 tctaccacct gcgcaattcg agcgacaact tccgcatcgc ccccggcatc aagggcatgg
      121 tgatgctggt gttcgacctg ccgagctttc cctacgtgtt caaggtcatc aaggacttct
      181 tccccgagca gaaggacacg acgcgcgagc tgatcaagcg caagtacctg atcgtgaagc
      241 agcacgaccg cgtcgggcgc atggccgaca cgctcgagta cagcaacgtc gcctttccgc
      301 gcgaacgctt cgaggacgaa ctcgtcgccg agctgaagca cttctgcccc agcctgctcg
      361 aggaagacgc cgacgcgctg gtgatccgcc acgtctacat cgagcggcgc atgatcccgc
      421 tcaacatcta cctgcaggag gcgtcgctgg cgcagatcga ggccgcggtg atcgagtacg
      481 gcaacgcgat caaggacctg ctgcgcgcca acatcttccc cggcgacatg ctgtggaaga
      541 acttcggcgt cacgcgccac ggcaaggtcg tgttctacga ctacgacgag atcgagtacc
      601 tgaccgagtg caacttccgc aaggtgccga cgccgcgcaa cgaggaggaa gagatgtcgg
      661 ccgaggtctg gtacaaggtc ggcccgaagg acgtcttccc cgagaccttc ggcccgttcc
      721 tgctcggcaa cccgggcgtg cgccaggtct tcatgaagca ccatgccgag ctgctcgagg
      781 cctcgttctg gcaaggccac aaggagcgca tcctggccgg gtatgtgcat gatgtctttc
      841 cgtacgatgc ccacaagcgc ttccagcacc ggcgcacgcg cgtcgccgcc acctgattcc
      901 gccgccaccg aaaggagccc accatggccg agtccatcgt catcgtttcc gccgcgcgca
      961 cgccgatcgg cggcctgctc ggcgactttt cttcgctgtc cgcgcaccag ctcggctcga
     1021 tcgcgatcaa ggccgcggtc gagcgtgccg gcatcaccgg cgacgccgtc gacgaggcgc
     1081 tgatgggcaa ctgcctgatg gccggccagg gccaggcgcc ggcgcgccag gcggtgctcg
     1141 gcgccgggct gccgcagtcg gtcggcgccg tcacgctgtc gaagatgtgc ggctccggca
     1201 tgcgcgcgct gatgttcgcg cacgacatgc tctcgagcga gaccgcgaac gtgatgatcg
     1261 ccggcggcat ggagagcatg accaacgcgc cgcacctgac gccgatgcgc aagggcgtga
     1321 agtacggcat ggcggcgctg tacgaccaca tggcgatcga cggcctcgaa gacgcctacg
     1381 aaagactgcc gaacggggcc ggcaaggcga tgggcgtgtt cgccgaagac tgcgtctcga
     1441 agtacaagtt cagccgcgag cagatggacc agttcgcggt cgattcgacc aggcgcagca
     1501 agcaggccaa cgaggacggc agcttcgact gggagatggc gccggtcacg ctgtcgggcc
     1561 cgaagggcga caccgtgatc aagttcgacg agcagccctt caaggccaag ctcgacaaga
     1621 tcgccggcct gaagcctgcc ttcaggaagg atggcacgat caccgccgcg acctcgtcca
     1681 gcatctccga cggcgccgcc gcgctggtgt tgatgcgcga gagcaccgct tcgagcctcg
     1741 gctgcacgcc gctcgcgcgc atccgcgggc acgcggtgca cgcccaggcg ccgaactggt
     1801 tcacgaccgc gccggccggg gcgatcgaga agctgctgaa gcgaagcggc tgggccgcga
     1861 aggacgtcga cctgtgggaa gtgaacgagg ccttcgccgc cgtcacgctc gcggcgatga
     1921 ccgagttcaa gctgtcgcac gagatcgtca acgtgcacgg cggcgcggtc gcgctcggcc
     1981 acccgatcgg cgcgagcggt gcgcgcatcg tcgtgaccct gctcggtgcg ctgcgcaagc
     2041 gcggcctgaa aaaaggcgtg gccgcgctgt gcatcggcgg cggcgaagcg acggcgctgg
     2101 cgctggaact gatctgaatg gacaaaccgc tggacccgcg cctcatcgcc gtcggcgaga
     2161 aggtcacgcg gcgcatgcgc tactcgcgcg ccgacatcgc gcagttcgcg cgcatgagcc
     2221 tggacgagaa cccgctccac ctggactcga tggtggcgca gcgggcgcgc ttcggcgaga
     2281 tcgtcgcgag cggccagcag accgcggcca tcctgatggg catgctggcc acccactact
     2341 cgcgcagcga cgacggcgtg gcgcggcaga tgatctgcct gaacatgaat ttcgcgttca
     2401 aggcgccggt gttcgccgag caggagatcg tgctgcagtg gaaggtgtcg tcggtcacgc
     2461 ccaacgccaa gctcaagggc ctggtggg
//
LOCUS       DATMJB010000441         3077 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_6151, whole genome shotgun sequence.
ACCESSION   DATMJB010000441 DATMJB010000000
VERSION     DATMJB010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6151"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VNU48_09480"
     CDS             <1..508
                     /locus_tag="VNU48_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI11547.1"
                     /translation="INPGDDKARYDYLRALLGAGHIDEARHAYAPVASKVMLDARLAA
                     AGHWLAACEVAAQSPNPNEFTAAIAANKRDFDARFALAQHHFANQRFTEAMDELLEIV
                     MRDKAWRDELARKTYVAILELMSRPAPSKIEAAVPKGTVELAGKAAAAPSDPVIDAYR
                     RKLSMALF"
     gene            complement(525..1634)
                     /locus_tag="VNU48_09485"
     CDS             complement(525..1634)
                     /locus_tag="VNU48_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11548.1"
                     /translation="MKSRLLSRLEDDIRSATQTLAADRLRCERAAYLARLGEFDEATR
                     EIAAVHQRHEARPNAAISVWLNLAHGLVSHYRELGSQAHDRVRRAHAMSVAAGLTDLQ
                     ALSAAWLAHLDYLRMNATGAAGHAAEALRLARSDHHAAQARACLVVAQTYDEAGRLDR
                     ARPWYDQAHRHATAEGDDATLSAMLWNKASLRVAALRQAVASGQVGAAAEAQALLSAE
                     STANFDAMLGVRSLRALQPILRAQTCSLLGRTDEALALYEQHLTEALDQGLRSIEGTL
                     VADRAWCRLQARNISGASEDARLAEGCLAREAPFGDRAPGHSRLTQVWEALGDHAAAE
                     RQRQLSLHAWQGHARDQANLLEALDKALAAAPPVS"
     gene            complement(1631..2545)
                     /locus_tag="VNU48_09490"
     CDS             complement(1631..2545)
                     /locus_tag="VNU48_09490"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWI11549.1"
                     /translation="MTASTPRPSSLLESRLHSLPLLARGKVRDNYAVGADRLLMIATD
                     RLSAFDVVMGEPIPGKGVLLTQMAMFWFAHLEGIVPNHLTGDDPVGVVAEDERDQVRG
                     RSMLVKRLKPLPVEAVVRGYLAGSGWAEYRKSGSVCGVALPEGLRNASRLPSPIFTPA
                     TKAEAGDHDENIDFSRMSAAIGAELAQRVRAVSIELYTAAAAFALKKGIIIADTKFEF
                     GLDAAGTLTLMDEVLTPDSSRFWPVESYADGSNPPSYDKQFVRDWLEQARVDDQPWNK
                     RAPAPALPAAVIDQTAARYREALDRLTA"
     gene            complement(2670..>3077)
                     /locus_tag="VNU48_09495"
     CDS             complement(2670..>3077)
                     /locus_tag="VNU48_09495"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWI11550.1"
                     /translation="IHRRIPNTHLVMHGSSSVPQELLAEIRLFGGDMKETYGVPVEEI
                     QEAIKHGVRKINIDTDVRLAMTAAIRQYMAQNPDKFDPRDYLKPAREAAKKICRQRYV
                     EFGCEGQAGKIKAQSLTQVAQRYSSGELAQTVV"
BASE COUNT          447 a         1078 c         1071 g          481 t
ORIGIN      
        1 catcaatccg ggcgacgaca aggcgcgcta cgactacctg cgtgcgctgc tcggcgccgg
       61 acacatcgac gaggcgcggc atgcctacgc gccggtggcg tcgaaggtga tgctcgacgc
      121 ccggctggcc gccgcaggtc actggctggc ggcttgtgaa gtcgccgcac aatccccgaa
      181 cccgaacgaa ttcaccgctg cgatcgccgc aaacaagcgc gatttcgacg cccgcttcgc
      241 gctcgcgcag caccacttcg cgaaccagcg tttcaccgag gcgatggacg aattgctcga
      301 gatcgtgatg cgcgacaagg cgtggcgcga cgaactcgcg cgcaagacgt acgtggcgat
      361 cctcgaactg atgagccggc ccgccccgag caagatcgaa gctgcggtcc cgaaaggcac
      421 cgtggaactt gccggcaaag ccgccgcggc gccgtccgac ccggtgatcg atgcctaccg
      481 gcgcaagctc agcatggcgc tgttctgaac cctgaaccga acggtcacga caccggcggc
      541 gcggcggcga gggccttgtc cagggcctcg agcaggttcg cctggtcccg ggcgtggccc
      601 tgccatgcat gcagcgagag ttggcgctgc cgctcggcgg cggcgtggtc gccgagcgcc
      661 tcccacacct gcgtcaaccg gctgtggccc ggcgcccgat cgccgaacgg ggcttcgcgg
      721 gcgaggcagc cctcggcgag gcgcgcgtcc tcggacgcgc cagagatgtt tcgagcctga
      781 agccggcacc atgcgcggtc ggccaccagc gtgccctcga tcgaccgcag gccctggtcc
      841 aaggcctcgg tcaggtgctg ctcgtacagc gccagcgctt cgtccgtccg gccgagcagg
      901 ctgcaggtct gggcacgcag gatcggctgc agcgcgcgca ggctgcgcac gccgagcatc
      961 gcatcgaagt tggcggtcga ttcggccgac agcagcgcct gcgcctcggc ggccgcgccc
     1021 acctgaccac tggccacagc ctggcgcagc gcggccaccc gaagcgacgc cttgttccac
     1081 agcatcgcgc tgagtgtcgc gtcgtcgccc tcggcagtgg cgtggcggtg cgcctggtcg
     1141 taccagggcc gcgcacggtc gagccggccg gcttcgtcgt acgtctgcgc caccacgagg
     1201 caggcgcgcg cctgtgcggc gtgatggtcg gaccgcgcca gtcgcagcgc ttcggctgca
     1261 tgccccgccg ccccggtcgc gttcatgcgc aggtagtcga ggtgcgcgag ccaggccgcg
     1321 ctcagcgcct gcagatccgt cagcccggcc gccacgctca tcgcatgggc gcgccggacg
     1381 cggtcgtgcg cctgcgaccc gagctcgcgg tagtggctga ccaggccgtg tgccaggttc
     1441 agccagacgc tgatcgccgc gttcgggcgc gcctcgtggc gctggtgcac ggcagcgatc
     1501 tcgcgcgtcg cctcgtcgaa ctcaccgagc cgggcgaggt aggccgcgcg ctcgcagcgc
     1561 aaccggtcgg ccgcgagcgt ttgagtcgca ctcctgatgt cgtcttcgag tcgactcagg
     1621 aggcgcgact tcatgcagtc aagcgatcga gcgcttcccg atagcgagcg gccgtctggt
     1681 cgatcacggc ggccggcagc gccggcgccg gcgcgcgctt gttccagggc tggtcgtcga
     1741 cgcgcgcctg ctcgagccag tcgcgcacga actgcttgtc gtagctcggc ggattgctgc
     1801 cgtcggcata gctttccacc ggccagaagc gcgaggagtc gggcgtcagc acctcgtcca
     1861 tcaaggtgag cgtgccggcg gcatcgagac cgaactcgaa cttggtgtcg gcgatgatga
     1921 tgcccttctt cagcgcgaac gcggccgccg ccgtgtagag ctcgatcgac accgcccgga
     1981 cgcgctgcgc caactcggcg ccgatggccg cgctcatgcg actgaagtcg atgttctcgt
     2041 cgtggtcgcc agcctcggcc ttggtggcgg gcgtgaagat cggcgatggc agcctcgagg
     2101 cgttgcgcag gccctcgggc agggccacgc cgcagacgga tccgctcttg cggtattccg
     2161 cccagccgct gccggccagg tagccgcgca cgaccgcctc caccggcaac ggcttcagcc
     2221 gcttgaccag catcgagcga ccgcgcacct ggtcgcgttc gtcctcggcc acgacgccga
     2281 ccgggtcgtc accggtcagg tgattcggca cgatgccttc gaggtgcgcg aaccagaaca
     2341 tcgccatctg cgtgagcagg acgcccttgc ccgggatcgg ctcgcccatc acgacatcga
     2401 aggcgctgag gcgatcggta gcgatcatca gcaggcgatc ggcgccgacc gcgtagttgt
     2461 cgcggacctt gccgcgcgcc agcagcggca gcgagtgcag ccggctttcg agcagcgacg
     2521 acgggcgtgg cgtggaagca gtcatggggt ggtgggcgct gggacgcgcc gagtaacgaa
     2581 ttgtatggac ggcgtcgagc cgcgaatcct gcatcctgtg gatgccatcg cgggtggcgg
     2641 gcctcggccc gcgcatcggc gcccgacgtt cagaccacgg tctgcgccag ttcgccgctc
     2701 gagtagcgct gcgccacctg ggtcagcgac tgcgccttga tcttgccggc ctgcccctcg
     2761 cagccgaact cgacatagcg ctgccggcag atcttcttgg ccgcctcgcg cgccggcttg
     2821 aggtagtcgc gcggatcgaa cttgtcgggg ttctgggcca tgtactgacg gatcgccgcg
     2881 gtcatggcca ggcgcacgtc ggtgtcgatg ttgatcttgc gcacgccgtg cttgatcgcc
     2941 tcctggatct cctcgaccgg cacgccgtag gtctccttca tgtcgccgcc gaagaggcgg
     3001 atctcggcga gcagctcctg cggcacgctc gacgagccgt gcatcaccag gtgggtgttc
     3061 gggatccggc ggtggat
//
LOCUS       DATMJB010000442         2097 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_74248, whole genome shotgun sequence.
ACCESSION   DATMJB010000442 DATMJB010000000
VERSION     DATMJB010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74248"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VNU48_09500"
     CDS             <1..576
                     /locus_tag="VNU48_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HWI11551.1"
                     /translation="IDWSRDVWGYVSLGYFKQATRAGEIGSSTMPHKVNPIDFENAEG
                     NFGLANALLTHLSQKLPVSRWQRDLSDSTVLRNMGVALGYALLGYDSLLRGLDKLEIN
                     EAALADDLDHAWEVLAEPIQTVMRRYGLPNPYERLKELTRGKAMTREAVRAFIETLEI
                     PVADRERLLALTPAGYTGQAVALARRIGGAA"
     gene            576..1415
                     /gene="pyrF"
                     /locus_tag="VNU48_09505"
     CDS             576..1415
                     /gene="pyrF"
                     /locus_tag="VNU48_09505"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HWI11552.1"
                     /translation="MDFRAQLARAETLNDSMLCVGLDPDPAKFPGSWKDDPGRIFDFC
                     ATIVDATRDLVIAYKPQIAYFAAQRAEEQLERLIAHIHRVAPEVPVILDAKRGDIGST
                     AEQYAREVFERYRADAVTLSPLMGFDSIEPYLAYEGRGAFLLCRTSNPGGADLLAQRL
                     SNGDRLFEHIARLAQGAWNGSGQLALVVGATFPAEIERVRELAPTLPLLIPGIGAQGG
                     DAAATVRAGWRADAGGRTVGPIVVSSSRAILYASRGDNFAQAARTAAQATRAELNRAR
                     RPA"
     gene            1539..>2097
                     /locus_tag="VNU48_09510"
     CDS             1539..>2097
                     /locus_tag="VNU48_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11553.1"
                     /translation="MKITRLADAELPESAARIIGRFYFGTNAAAGLASAAILSTLLPQ
                     LPLAHRVWLGGSSALFGLLCLLALRFSAHPRFPMHAALCGVGAIAMALTGFFSVLLGE
                     GLRGPPLAFCGLIVCVVGSITHGRASLALGAFAALEMTLLAWLEARGLIHGRAAATPL
                     GMVLFYQWLVVLCGTIGGSLIARVLA"
BASE COUNT          283 a          768 c          742 g          304 t
ORIGIN      
        1 atcgactggt cgcgcgacgt ctggggctat gtcagcctgg gctacttcaa gcaggccacg
       61 cgcgccggcg agatcggctc gtcgacgatg ccgcacaagg tgaacccgat cgacttcgag
      121 aacgccgaag gcaacttcgg cctggccaac gcactgctca cgcacctgtc gcagaagctg
      181 ccggtgagcc gctggcagcg cgacctgagc gacagcacgg tgctgcgcaa catgggcgtg
      241 gcgctgggct acgcgctgct cggctacgac tcgctgctgc gcggcctgga caagctcgag
      301 atcaacgagg ccgcgctcgc cgacgacctc gaccacgcct gggaagtgct ggccgaaccg
      361 atccagaccg tgatgcgccg ctacggcctg cccaacccct acgagcgcct gaaggagctg
      421 acgcgcggca aggcgatgac gcgcgaggcg gtgcgcgcct tcatcgagac gctcgagatc
      481 ccggtcgccg atcgcgagcg gctgctcgcg ctcacaccgg ccggctacac cgggcaggcg
      541 gtcgcgctgg cgcggcgcat cggcggagcg gcgtgatgga cttccgcgcg cagctggccc
      601 gcgccgagac gctcaacgac tcgatgctgt gcgtcggcct cgaccccgat ccggcgaagt
      661 tccccggctc gtggaaggac gacccgggcc gcatcttcga cttctgcgcc accatcgtcg
      721 atgcgacccg cgatctggtg atcgcctaca agccgcagat cgcctacttc gcggcgcagc
      781 gcgccgaaga gcagctcgag cggctgatcg cgcacatcca ccgggtcgcg cccgaggtgc
      841 cggtgatcct ggacgcgaag cgcggcgaca tcggcagcac cgccgagcaa tacgcgcgcg
      901 aggtgttcga gcgctaccgc gccgatgccg tgaccctgtc gccgctgatg ggcttcgatt
      961 cgatcgagcc ctatctcgcc tacgagggca ggggcgcgtt cctgctgtgc cgcacctcga
     1021 acccgggcgg cgccgatctg ctggcgcagc ggctgtcgaa cggcgaccgg ctgttcgagc
     1081 acatcgcgcg cctcgcgcag ggcgcgtgga acggcagcgg ccagctcgcg ctggtggtcg
     1141 gcgcgacctt cccggccgag atcgagcgcg tgcgcgaact cgcaccgacc ttgccgctgc
     1201 tgatccccgg catcggcgcg cagggcggcg acgccgccgc cacggtcagg gccggctggc
     1261 gtgccgacgc cggcgggcgc acggtcgggc cgatcgtcgt cagctcgtcg cgcgcgatcc
     1321 tctacgcgag ccgcggcgac aacttcgcgc aggcggcgcg caccgccgcg caggcgaccc
     1381 gggcggaact gaaccgggcc cgccggccgg cctgagcggc accgacgcgg ccggcgacca
     1441 gcggcatcgc gacaaatggc cgttgcccca gccgctgttc cggggctggc cgcggggcca
     1501 ggctcctaac atccgcggac cgtcgcacgc cccgttgcat gaagatcacc cgtctggccg
     1561 atgccgaact gcccgaatcc gcagcgcgga tcatcgggcg tttctacttc ggcaccaacg
     1621 cggccgccgg gctggcgagc gcggcgatcc tgtcgaccct gctgccgcag ctgccgctgg
     1681 cacatcgcgt gtggctgggc ggcagctcgg ccctgttcgg gctgctgtgc ctgctggcac
     1741 tgcgcttcag cgcgcatccg cgcttcccga tgcatgccgc gctgtgcggc gtcggcgcga
     1801 tcgcgatggc cttgaccggc ttcttcagcg tgctgctcgg cgagggcctg cgcggcccgc
     1861 cgctcgcgtt ctgcggcctg atcgtgtgcg tcgtcggctc gatcacgcac gggcgcgcga
     1921 gcctggcgct cggcgccttc gcggcgctcg agatgacgtt gctcgcatgg ctcgaggcgc
     1981 gcggcctgat ccacggccgc gccgcggcca cgccgctggg catggtgctg ttctaccagt
     2041 ggctggtcgt gctgtgcggc acgatcggcg gcagcctgat cgcgcgcgtg ctcgccc
//
LOCUS       DATMJB010000443         2620 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_422489, whole genome shotgun sequence.
ACCESSION   DATMJB010000443 DATMJB010000000
VERSION     DATMJB010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422489"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VNU48_09515"
     CDS             <1..544
                     /locus_tag="VNU48_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWI11554.1"
                     /translation="DKRATLDLAIEHLLQQSSVAPEAIALPAPASPLSARLFGGLVVD
                     ADRCTMCLSCVSACPEAALADNPDKLQLRFIEKNCVQCGLCVRTCPEDAITLEPRLWL
                     ADAGKARKQLRVLNEVEPYRCIRCNKPFGTPKGIELMVAKLGAHPMFQGAGAERLRMC
                     GDCRVIDIHTNPNEVRITDL"
     gene            563..1195
                     /locus_tag="VNU48_09520"
     CDS             563..1195
                     /locus_tag="VNU48_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HWI11555.1"
                     /translation="MADAAAALRFSTPDDREEIARAEVYGLLASLYHAAPEADLYEQL
                     RVAVTEAPSAGAFLETSWSELVATSRRLSLAAVGDEFVALFGGVGKPEIFPYGSWFIA
                     GSLNEKPLVELRDALAALGLERPAGVLETEDHIANLCEVMRYLIAGDDVGVSNLGVQQ
                     RFFDAQLRPWVERLCDTIAAHPRADFYRALAGFTRDFFAVEAQGFDLLDA"
     gene            1368..1595
                     /locus_tag="VNU48_09525"
     CDS             1368..1595
                     /locus_tag="VNU48_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HWI11556.1"
                     /translation="MSDRAGDTPTPSTPLKRRGLLIGAGAAGAAVLAVKALPGAAPAA
                     PVAVAAKALADTSGGYQVTAHVLRYYETTRS"
     gene            1604..>2620
                     /locus_tag="VNU48_09530"
     CDS             1604..>2620
                     /locus_tag="VNU48_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWI11557.1"
                     /translation="MLLTRKSNHAGSRSSGLVDSLARGLDTHNRRAIPTMDRRAFLRR
                     SGMGVGAGLAATQLTLVQRSAHAADAPADAAARKVQVKRTVCGHCSVGCAVDAVVENG
                     VWVRQEPVFDSPINLGAHCAKGAALREHGHGEYRLRTPMKLQGGKYVKIGWDEALNEI
                     SAKMLELKKQSGPDSIFIVGSSKHNNEQAYLLRKWVSFWGSNNCDHQARICHSTTVAG
                     VANTWGYGAMTNSYNDMRNSKVAMYIGSNAAEAHPVSMLHLLHAKENGCKVIVADPRY
                     TRTAAKADKYVRFRSGTDVPLIFGVLHHIFKNGWEDKEYIRQRVYGMEDVKKEVLAKW
                     TPDKV"
BASE COUNT          451 a          884 c          898 g          387 t
ORIGIN      
        1 tgacaagcgc gcgacgctgg accttgcgat cgaacacctg ctgcagcagt cgagcgttgc
       61 gcccgaggcg atcgcgttgc cggcgccggc aagtcccctc tctgctcgtc tattcggcgg
      121 tctggtggtc gacgccgatc gctgcacgat gtgcctgagc tgcgtcagcg cctgccccga
      181 ggcggcgctg gccgacaacc ccgacaagct gcagctgcgc ttcatcgaga agaactgtgt
      241 gcagtgcggg ctgtgcgtgc gcacctgccc cgaagacgcg atcacgctgg agccgcgcct
      301 gtggctcgcc gatgctggca aggcgcgcaa gcagctgcgg gtgctcaacg aggtcgagcc
      361 gtatcgctgc atccgctgca acaagccctt cggcacgccg aagggcatcg agctgatggt
      421 ggccaagctc ggcgcgcacc cgatgttcca gggcgccggt gcggaacgtc tgcgcatgtg
      481 cggcgactgc cgcgtgatcg acatccacac gaacccgaac gaagtgagga tcaccgacct
      541 atgaacgaat tcggggccgc ccatggctga cgccgccgcg gcactgcgct tcagcacgcc
      601 cgacgaccgc gaagagatcg cccgtgccga ggtctacggc ctgctcgcga gcctgtacca
      661 cgcggcgccg gaggcggacc tgtacgagca gctgcgcgtc gcggtcaccg aggcgccgag
      721 cgcgggcgcg ttcctcgaga cgagctggtc cgaactcgtc gcgacctcgc ggcgcctgtc
      781 gctggctgcg gtcggcgacg aattcgtcgc gctgttcggc ggcgtcggca agcccgagat
      841 cttcccgtac ggctcgtggt tcatcgccgg cagcctgaac gagaagccgc tcgtcgagct
      901 gcgcgacgcg ttggctgcgc tcgggctgga acgcccggcc ggtgtgctcg agaccgagga
      961 ccacatcgcc aacctgtgcg aggtgatgcg ctacctgatc gccggcgacg atgtcggcgt
     1021 cagcaacctc ggcgtgcagc agcgcttctt cgacgcgcag ctgcggccct gggtcgagcg
     1081 cctgtgcgac acgatcgccg cgcacccgcg ggccgacttc taccgcgcgc tggccggctt
     1141 cacgcgcgac ttcttcgcgg tcgaagcgca ggggttcgac ctgctcgacg cgtaggactt
     1201 cgtgcagctt gcacgaaact gacaaggcgg tgcgccgcac ggtcaagtgc ttacggactg
     1261 accctgatgc ggccatgaac caacctgcat acatttggtg cagaccgcgc tgaccgagat
     1321 cagccggcac cttgttggga cccacgacct tctcggagca gaccgccatg agtgatcgag
     1381 ccggtgacac ccccacgccc tccacaccct tgaagcgtcg cggcctgctg atcggcgccg
     1441 gcgcggccgg cgccgcggtg ctggcggtca aggccctgcc cggcgcggcg ccggcggcac
     1501 cggtcgcggt ggccgcgaaa gcgctggccg acacgagcgg cggctaccag gtcacggcgc
     1561 acgtgctgcg ctactacgaa accactcgat cgtgagggtc gccatgctgc tgactcgcaa
     1621 atcgaatcac gccggatcac gctccagcgg tctggtcgat agcctcgcgc gcgggctgga
     1681 cacgcacaac cggcgggcga tcccgacgat ggaccggcgc gccttcctgc ggcgctcggg
     1741 catgggcgtc ggtgccggcc tcgcggccac gcagctgacg ctggtgcaga gatcagcgca
     1801 cgccgccgac gcaccggccg atgccgcggc gcgcaaggtg caggtcaagc gcacggtctg
     1861 cggccactgt tcggtcggct gtgcggtcga cgcggtggtc gagaacgggg tctgggtgcg
     1921 ccaggagccg gtgttcgatt cgccgatcaa cctcggcgcg cattgcgcga agggtgcggc
     1981 gctgcgcgag cacggccacg gcgaataccg cctgcgcacg ccgatgaagc tgcagggtgg
     2041 caagtacgtc aagatcggct gggacgaggc gttgaacgag atcagcgcga agatgctcga
     2101 gctgaagaag cagagcgggc ccgactcgat cttcatcgtc ggctccagca agcacaacaa
     2161 cgagcaggcc tacctgctgc gcaagtgggt cagcttctgg ggcagcaaca actgcgacca
     2221 ccaggcgcgc atctgccact cgaccaccgt ggcgggcgtg gcgaacacat ggggctacgg
     2281 cgccatgacc aattcgtaca acgacatgcg caactcgaag gtggcgatgt acatcggctc
     2341 gaacgcggcc gaggcccacc cggtgagcat gctgcacctg ctgcacgcca aggagaacgg
     2401 ctgcaaggtg atcgtcgccg acccgcgcta cacgcgcacc gccgccaagg ccgacaagta
     2461 cgtgcgcttc agatccggca ccgacgtgcc gctgatcttc ggcgtgctgc atcacatctt
     2521 caagaacggc tgggaggaca aggagtacat ccgccagcgc gtctacggca tggaggacgt
     2581 gaagaaggaa gtgctggcga agtggacgcc cgacaaggtg
//
LOCUS       DATMJB010000444         3801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_302401, whole genome shotgun sequence.
ACCESSION   DATMJB010000444 DATMJB010000000
VERSION     DATMJB010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302401"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VNU48_09535"
     CDS             <1..426
                     /locus_tag="VNU48_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11558.1"
                     /translation="TEDRRARWEAYQALSPEQKQRLAARATPSADTVRKTAPATVRSD
                     KGARDSLPQAKSNIVPNPASVAPPRAVTSTVLQASPGATTTLITKRPTPPGHQQTGMP
                     KIAATPEFVNKATLLPQRGPQGAATRSAAAPASGNPPRQ"
     gene            456..956
                     /locus_tag="VNU48_09540"
     CDS             456..956
                     /locus_tag="VNU48_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWI11559.1"
                     /translation="MPAPSLMRRMASLVYEGLLLFGIGLIPGAIGALFVALTGQQHPL
                     QGELALQVITVLIYAVYFSWFWSARGQTLPMKTWHIRVVTAEGRPLSQARALARFVAG
                     CAWVAPAVLIAHLNGWSPRETLAAVGIGVVAYALLALLHPQRQFWHDAVCGTRLIDAP
                     PARATR"
     gene            998..1378
                     /locus_tag="VNU48_09545"
     CDS             998..1378
                     /locus_tag="VNU48_09545"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HWI11560.1"
                     /translation="MTNPHKGRTGIDRIVRAVGYSLTGLRLAYRGESAFRQEFWLAVV
                     LVPLAFWVGRNWIEVLLLAGSVSLVLIVELLNSAVEAAIDRVSFEWHELSKRAKDIGS
                     AAVFLSLLLCAGTWLAALWQRFVV"
     gene            1393..1977
                     /locus_tag="VNU48_09550"
     CDS             1393..1977
                     /locus_tag="VNU48_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWI11561.1"
                     /translation="MRPLSLCVYCGSRPGDLPAYADAARAVGTEIGRRGWQLVYGGGR
                     AGLMGVVADAALAAGAAVVGVIPQSLMERELGHAGLTELHVVDTMHQRKTMMAERSDA
                     FLALPGGIGTFEELFEVWTWRQLGYHDKPLGLLNAAGYYDALLGFLQHSRERGFMASS
                     QTDLLQVDADPVALVDRLAALAPLATAADDYRRT"
     gene            complement(1982..2320)
                     /locus_tag="VNU48_09555"
     CDS             complement(1982..2320)
                     /locus_tag="VNU48_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWI11562.1"
                     /translation="MKQITAIVKPFKLEEVREALGQVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKLEVVVKDSDLENCIEAIVKAAKTGKIGDGKIFVTAVEQVIRI
                     RTGETNDEAV"
     gene            complement(2353..>3801)
                     /locus_tag="VNU48_09560"
     CDS             complement(2353..>3801)
                     /locus_tag="VNU48_09560"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWI11563.1"
                     /translation="ARALADLPHLFVVVGHPHQFGDRGDVRSNSVSVPQRYNAASVLH
                     EGRVLATYCKRELPNYQVFDERRYFASGRDAGQGAMVFEVQGLSFGIVICEDAWFDEP
                     GRLAKAAGAQVLCVLNASPFHLGKLAEREQRMAQTARSLQMPLLYAHLAGAQDEVVFD
                     GASFALDADGAVGARAPMFDEALTLVEVTERSARGALAQVPAIEAQAWAALVVGVRDY
                     VDKNGFPGVIIGLSGGIDSALVLAIAVDALGPARVRTVMMPSPYTADISWIDARDMAQ
                     RLGVRYDEISIVPMFEDFKKSLAAEFAGRAEDATEENIQARIRGILLMALSNKFGAIV
                     LTTGNKSEMATGYCTLYGDMAGGFAVIKDVAKTLVYRLATWKNAQGREVIPERIITRP
                     PSAELKPDQKDQDSLPPYEVLDAILARYMEQDQGIDEIVAAGFAPADVERVTRLIKIN
                     EYKRRQAPVGIRITHRAFGRDWRYPITSKFRA"
BASE COUNT          548 a         1364 c         1275 g          614 t
ORIGIN      
        1 accgaggatc gccgggcacg ctgggaggcc taccaggcgc tttcgccgga acagaaacag
       61 cggctcgccg cgcgcgccac tccgagcgcc gacaccgttc gcaagacggc gccagcgacc
      121 gtgcgctccg acaagggcgc gcgcgattcg ctgccgcagg cgaagtccaa catcgtgccg
      181 aatccggcct cggtcgcgcc gcctcgcgcc gtgacatcga ccgtgctgca ggccagcccc
      241 ggtgccacga ccacgctgat cacgaagcgg ccgaccccac ccggccacca gcagaccggc
      301 atgccgaaga tcgctgccac acccgagttc gtgaacaagg cgacgctgct gccgcagcgc
      361 gggccgcagg gcgcggcgac gcgttcggcc gccgcgccgg cgagcgggaa ccctccccgg
      421 caatgacggt ggccgcggaa gaaggcgcga gcgccatgcc ggcgcccagc ctgatgcggc
      481 gcatggcgtc gctcgtctac gaaggtctgc tgctgttcgg catcggcttg attccgggcg
      541 cgatcggtgc cctgttcgtc gcgctgaccg ggcagcagca cccccttcag ggtgaactgg
      601 ctctgcaggt catcaccgtt ctgatctacg cggtgtactt cagctggttc tggtcggcac
      661 gcggccagac gctgccgatg aagacctggc acatccgcgt cgtcaccgcc gaaggtcggc
      721 ccttgagcca ggcgcgcgcg ctggcccgct tcgtcgccgg ctgcgcctgg gttgccccgg
      781 ccgtgctgat cgcgcacctg aacggctggt cgccgcgcga aacgctcgcg gcggtcggca
      841 tcggcgtggt cgcgtatgcg ctgctcgcgc tgctgcaccc gcagcgccag ttctggcacg
      901 acgcggtgtg cggcacgcgg ctgatcgacg cgcccccggc gcgcgccacg cgctgaacgc
      961 gccggcgcgt cacgggcgcg aggcacaatc cgcccccatg acgaatccgc acaagggtcg
     1021 caccggcatc gatcgaatcg tccgcgctgt cggttactcg ctcacgggcc tgcgcctggc
     1081 gtaccgcggc gagagcgctt ttcgccagga gttctggctc gcggtcgtgc tggtgccgct
     1141 cgccttctgg gtcgggcgca actggatcga ggtgctgctg ctcgcggggt cggtctcgct
     1201 ggtgctgatc gtcgagctgc tgaactccgc cgtcgaggcc gcgatcgacc gcgtctcgtt
     1261 cgagtggcac gagttgtcca agcgcgccaa ggacatcggc agcgccgcgg tattcctgtc
     1321 gctgctgctg tgcgccggca cctggctggc cgcgctgtgg cagcgcttcg tcgtgtgagc
     1381 acgagtcgag cgatgcgccc gctctcgttg tgcgtctact gcggctcgcg cccgggcgac
     1441 ttgcccgcgt atgccgacgc cgcgcgtgcc gtcggcaccg agatcgggcg ccgcggctgg
     1501 caactggtct atggtggtgg gcgcgccggg ctgatgggcg tggtggccga cgcggcgctg
     1561 gcggcgggtg ccgccgtcgt cggcgtgatc ccgcaatcgc tgatggaacg cgaactcggc
     1621 cacgccggcc tgaccgagtt gcacgtcgtc gacacgatgc accagcgcaa gacgatgatg
     1681 gccgagcgca gcgacgcgtt cctcgcgctg cccggcggca tcggcacctt cgaggagctg
     1741 ttcgaggtct ggacctggcg ccagctcggc taccacgaca agccgctcgg gttgttgaac
     1801 gccgcgggct actacgacgc gctgctcggc ttcctgcagc acagccgcga gcgcggcttc
     1861 atggcgagca gccagaccga tctgctgcag gtcgacgccg acccggtcgc cctggtggat
     1921 cgactggccg cgctcgcgcc gctcgcgacc gcggcggacg actatcgccg gacctgacgc
     1981 ctcagaccgc ctcgtcgttg gtctcgccgg tgcggatgcg aatgacctgc tcgaccgccg
     2041 tgacgaagat cttgccgtcg ccgatcttgc cggtcttcgc ggccttgacg atcgcctcga
     2101 tgcagttctc gagatcgctg tctttcacga cgacctcgag cttgaccttg ggcaggaagt
     2161 cgaccacgta ttcggcgcca cggtacagct cggtgtggcc cttctgacgc ccgaagccct
     2221 tcacctcggt caccgtcagg ccggtcacac ccacctgccc cagcgcctcg cgcacttctt
     2281 cgagcttgaa gggcttgacg atcgcggtga tctgtttcat ggcgagctcc agaaggtttg
     2341 atcgcaggta gtttacgcgc ggaacttcga agtgatcggg tagcgccagt cgcgcccgaa
     2401 cgcccggtgc gtgatgcgga tgcccaccgg cgcctggcgg cgcttgtact cgttgatctt
     2461 gatcaggcgc gtgacccgct ccacgtcggc cggcgcgaag cccgcggcga cgatctcgtc
     2521 gatgccctga tcctgctcca tgtagcgcgc caggatcgca tcgagcacct cgtagggcgg
     2581 caggctgtcc tggtccttct ggtccggctt gagctcggcc gacggcggcc gcgtgatgat
     2641 gcgctcgggg atcacctcgc ggccctgcgc gttcttccac gtcgccagcc ggtacacgag
     2701 cgtcttggcc acgtccttga tcaccgcgaa gccgccggcc atgtcgccgt acaaggtgca
     2761 gtagccggtc gccatctcgc tcttgtttcc ggtggtcagc acgatggcgc cgaacttgtt
     2821 cgacagcgcc atcagcagga tgccgcggat gcgcgcctgg atgttctctt cggtcgcatc
     2881 ctcggcccgg ccggcgaact cggcggccag gctcttcttg aagtcctcga acatcggcac
     2941 gatcgagatc tcgtcgtagc gcacacccag gcgctgcgcc atgtcgcgcg catcgatcca
     3001 cgagatgtcg gcggtgtacg gcgagggcat catcacggtg cgcacgcgcg ccggcccgag
     3061 cgcgtcgacc gcgatcgcca gcaccagcgc cgagtcgatg ccgcccgaca agccgatgat
     3121 cacgcccggg aaaccgttct tgtcgacgta gtcgcgcacg cccaccacca gcgccgccca
     3181 ggcctgcgcc tcgatggccg gcacctgcgc cagcgcgcca cgcgcactgc gctcggtcac
     3241 ctcgaccagc gtcagcgcct cgtcgaacat cggggcgcgg gcgccgacgg cgccgtcggc
     3301 gtcgagcgcg aacgaggccc cgtcgaacac gacctcgtcc tgtgcgcccg ccaggtgcgc
     3361 gtagagcagc ggcatctgca ggctgcgcgc cgtctgcgcc atgcgctgct cgcgctcggc
     3421 gagcttgccg aggtgaaacg gcgacgcgtt caacacgcac agcacctgcg cgccggcggc
     3481 cttggcgagc cggccgggct cgtcgaacca ggcgtcttcg cagatcacga tgccgaagct
     3541 taagccctgc acctcgaaca ccatcgcgcc ctggccggcg tcgcggcccg aggcgaagta
     3601 gcggcgttcg tcgaagacct gatagttcgg caactcgcgc ttgcagtagg tcgcgagcac
     3661 ccggccctcg tgcagcaccg aggccgcgtt gtagcgctgc ggcaccgaaa ccgagttgga
     3721 cctaacatcg ccccgatcac cgaactgatg cggatgcccg accaccacga acagatgcgg
     3781 caaatcggcc agtgcacgcg c
//
LOCUS       DATMJB010000445         3012 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_697023, whole genome shotgun sequence.
ACCESSION   DATMJB010000445 DATMJB010000000
VERSION     DATMJB010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697023"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1134)
                     /locus_tag="VNU48_09565"
     CDS             complement(103..1134)
                     /locus_tag="VNU48_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWI11564.1"
                     /translation="MTTAIPATLIPGDGIGPEIVDATLAALDALRAPFEWDRQIAGLG
                     GVRSAGDPLPEATLASIRRTRLALKGPLETPSGGGYRSSNVRLREEFQLYANLRPART
                     VIPGGRFDKIDLMVVRENLEGLYIGHEHYVQIDDDPHAVAIGTGINTRAGSRRLLEYA
                     FETALATGRRKVTIVHKANIMKALTGIFLETGLELYERQYKGKFELDTVIVDACAMKL
                     VLNPWQFDMLVTTNLFGDILSDLVAGLVGGLGMAPGANIGTDAAIFEAVHGSAPDIAG
                     KGLANPIALLLAAALMLDHVKRPELATRLRGAIDATLNLDQVRTGDLGGSASTAAFTQ
                     ALVARIANG"
     gene            complement(1219..2436)
                     /locus_tag="VNU48_09570"
     CDS             complement(1219..2436)
                     /locus_tag="VNU48_09570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HWI11565.1"
                     /translation="MNSGVAVTGLGAIAPHGDDPAALFAALLRGESTIRPVFPELARP
                     AAAATVTFDASRWFTKLQLAGVDRVSQLAVAAADLALRDAGIDATAARPIDPERIGVY
                     VGCGMGGAAALETAYHGGGRVPPLTIPAFMPNAPAAHVAMRQRVQGPVLTYSVACASS
                     AVAIAEAAAAVQRGDIDLAIAGGSEALIVPGVMLGWQAMQTLASFEPGAAATAVRPFA
                     TDRSGFVLGEGAAFVVLESSEHARRRGARIRSTLAGWGLSSDATHLTKPDAPGQARAL
                     RAALRRADLAPRDVGYCNAHGTATRIGDVVERDALALVWGDALDTLRVSSTKALHGHL
                     LGAAGALEAVIAVLALEHEALPPNAHCAEVDPACRLNLVAPGATAAPGLRAAISNSFA
                     FGGTNSVLLFTRA"
     gene            complement(2433..2672)
                     /locus_tag="VNU48_09575"
     CDS             complement(2433..2672)
                     /locus_tag="VNU48_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWI11566.1"
                     /translation="MSSLQDLQALIHEKFGLDPAELDPHASMREKGVDSLALVEFLFA
                     IEDRFGISLSDKDSNVDTLAELAVVVDKARATQAA"
     gene            complement(2669..>3012)
                     /locus_tag="VNU48_09580"
     CDS             complement(2669..>3012)
                     /locus_tag="VNU48_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWI11567.1"
                     /translation="AGFGVLKGVDAAGNACPSLHVAVAIFTAIWIDHVLRQARAPLAL
                     RAVNIGWFALIAWSTLAIKQHVVLDVAAGMLLGVAFALPSLHWRPARPRGLVPRVRAD
                     IIERMSTDIAP"
BASE COUNT          419 a         1070 c         1109 g          414 t
ORIGIN      
        1 gcgcagccca agcgcggcgt cgtcgtcgac atgaccgcgc agatgctgga catcgcgcgc
       61 caactgaacg cggtcgtgct gagctgagga gcccgccgca cctcagccgt tggcgatgcg
      121 cgcgaccagt gcctgcgtga acgccgcggt gctggcgctg ccgccgaggt cgccggtgcg
      181 cacctggtcg aggttcagcg tcgcgtcgat cgcgccgcgc agccgcgtcg cgagttcggg
      241 ccgcttcacg tggtcgagca tcagcgcggc agcgagcagc agcgcgatcg ggttcgccag
      301 gcccttgccg gcgatgtcgg gcgccgagcc gtgcaccgct tcgaagatcg ccgcgtcggt
      361 gccgatgttc gcgcccggcg ccatgcccag gccgccgacc agcccggcga ccagatccga
      421 caggatgtcg ccgaacaggt tcgtcgtcac cagcatgtcg aactgccacg ggttgagcac
      481 gagcttcatc gcgcaggcgt cgacgatcac ggtgtcgagc tcgaacttgc ctttgtactg
      541 acgctcgtac agctcgaggc cggtttccag gaagatgccg gtcagcgcct tcatgatgtt
      601 ggccttgtgc acgatcgtga ccttgcgccg cccggtcgcc agcgcggtct cgaacgcgta
      661 ctcgagcagg cgccggctgc cggcgcgcgt gttgatgccg gtgccgatcg cgaccgcgtg
      721 cgggtcgtcg tcgatctgca catagtgctc gtggccgatg tacaggccct cgaggttctc
      781 gcgcaccacc atcaggtcga tcttgtcgaa gcgcccgccc ggaatgacgg tgcgcgccgg
      841 gcgcaggttg gcgtagagct ggaactcctc gcgcaagcgc acgttcgacg agcggtagcc
      901 gccgcccgag ggcgtctcga gcggcccttt cagcgccagc cgcgtgcggc ggatgctcgc
      961 cagcgtcgct tcgggcagcg ggtcgccggc cgagcgcacg ccgccaagac cggcgatctg
     1021 gcggtcccac tcgaacggtg cgcgcaaagc gtcgagcgcg gccagcgtcg catcgacgat
     1081 ctcggggccg atgccgtcgc cggggatcag ggtcgcgggg atggcggtgg tcatcgggtg
     1141 atctccttca tctgggcgtg ggcgccgcgc gcccgtgcgg ggtcgtcgaa gcatacgccg
     1201 cgccagcgcg gtggccgctc aggcgcgggt gaacagcagc accgagttcg tgccgccgaa
     1261 cgcgaacgag ttgctgatcg cggcgcgcag ccccggcgcg gcagtggcgc cgggtgcgac
     1321 caggttcagg cggcacgccg ggtcgacctc ggcgcagtgc gcgttcggcg gcagcgcctc
     1381 gtgctcgagc gcgagcaccg cgatcaccgc ttcgagcgcc ccagccgcgc ccagcaggtg
     1441 gccgtgcagc gccttggtcg agctgacgcg cagcgtgtcg agcgcgtcgc cccagaccag
     1501 cgcgagggcg tcgcgctcga cgacgtcgcc gatgcgtgtc gcggtgccgt gcgcattgca
     1561 gtagccgaca tcgcgcggtg ccaggtcggc gcggcgcagc gcggcgcgca gcgcgcgcgc
     1621 ctggcccggc gcgtcgggct tggtcaggtg cgtcgcgtcg ctgctcaggc cccagccggc
     1681 gagcgtcgag cggatgcgtg cgccgcgccg gcgcgcgtgc tcgctcgatt cgaggacgac
     1741 gaaggccgcg ccctcgccga gcacgaagcc gctgcggtcg gtggcgaacg gccgcaccgc
     1801 cgtggccgcg gcgcccggct cgaagctggc gagcgtctgc atcgcctgcc atccgagcat
     1861 gacgccgggc acgatcagcg cctcgctgcc gccggcgatg gccagatcga tgtcgccgcg
     1921 ctgcaccgcc gcggccgcct cggcgatcgc caccgccgac gacgcgcacg cgaccgagta
     1981 ggtcagcacc gggccctgca cgcgctggcg catcgcgacg tgggccgccg gcgcgttcgg
     2041 catgaacgcc ggaatcgtca gcggcggcac gcgcccgccg ccgtggtagg ccgtctcgag
     2101 cgccgcggcc ccgcccatgc cgcagccgac gtagacgccg atgcgctcgg ggtcgatggg
     2161 gcgcgccgcg gtggcgtcga tgccggcatc gcgcagcgcc agatcggcgg cggccaccgc
     2221 aagctggctg acgcggtcga cgccggcgag ctgcagcttc gtgaaccagc gcgatgcgtc
     2281 gaaggtcacg gtcgcggcgg cggccggcct ggccagctcg ggaaacaccg gccggattgt
     2341 cgattcgccc cgcagcagcg ccgcgaacag cgcggcgggg tcgtcgccgt gcggcgcgat
     2401 cgcgccgagg ccggtgacgg cgacgccgga attcacgccg cctgcgtcgc gcgcgccttg
     2461 tcgaccacga ccgcgagctc ggccagcgtg tcgacgttgg aatccttgtc ggacagcgag
     2521 atgccgaaac ggtcttcgat cgcgaacagg aactcgacca gtgcgagcga atcgacgccc
     2581 ttttcgcgca tcgacgcgtg cggatcgagt tcggccggat cgaggccgaa cttctcgtgg
     2641 atcagggcct gcaggtcttg cagagagctc atggtgcgat gtccgtcgac atgcgctcga
     2701 tgatatccgc tcgtactcgc ggaaccagcc cgcgcggtcg cgccgggcgc cagtgcagcg
     2761 agggcagcgc gaacgcgacg cccagcagca tgccggcggc gacgtcgagc acgacgtgct
     2821 gcttgatcgc cagcgtcgac cacgcgatca gcgcgaacca gccgatgttc acggcgcgca
     2881 gcgccagcgg tgcgcgcgcc tggcgcagca cgtggtcgat ccagatcgcg gtgaagatcg
     2941 ccaccgccac gtgcagcgac gggcaggcgt tgcccgccgc gtcgacaccc ttgagcacgc
     3001 cgaagccggc ga
//
LOCUS       DATMJB010000446         2960 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_841230, whole genome shotgun sequence.
ACCESSION   DATMJB010000446 DATMJB010000000
VERSION     DATMJB010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841230"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /gene="purU"
                     /locus_tag="VNU48_09585"
     CDS             <1..159
                     /gene="purU"
                     /locus_tag="VNU48_09585"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587058.1"
                     /note="produces formate from formyl-tetrahydrofolate which
                     is the major source of formate for PurT in de novo purine
                     nucleotide biosynthesis; has a role in one-carbon
                     metabolism; forms a homohexamer; activated by methionine
                     and inhibited by glycine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HWI11568.1"
                     /translation="IEQDVERVDHSRGPEDFTAVGRDVESVVLARAVRWHVEHRVLLN
                     GRKTVVFT"
     gene            201..644
                     /locus_tag="VNU48_09590"
     CDS             201..644
                     /locus_tag="VNU48_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWI11569.1"
                     /translation="MARPKPVAPLASPDEIEQQFYEALQRGDLERLMAVWSDDEEISC
                     VHPGGPRVVGATAIRSAFESIFANGAVNAQPEKVRRLQTHACSVHSVLEHIRVMTTEG
                     PQSAWVVATNVYLLGAQGWRLVSHHASPGTPREVQEIVEAASVLH"
     gene            658..1644
                     /locus_tag="VNU48_09595"
     CDS             658..1644
                     /locus_tag="VNU48_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI11570.1"
                     /translation="MQQYRAPRWLIGGHAQTIWPALFSKRFDGPVPSFRRERWATPDG
                     DFIDVDWQEVSAPGSPGTATAPMLVLFHGLEGSSASHYSQAFAHWARGHGWRFVVPHF
                     RGCSGELNRAPRAYHSGDFEEIGWMLRRLRAQHEGRIAAVGVSLGGNALLRWAEEAGE
                     SAAQTVGAVAAVSSPVDLAAGGHAIGIGLNRQVYTRMFLRTMKPKALAKLAQHPGLFD
                     RDKLLAARTLYEFDNVFTAPLHGFRDTDDYWSRGSAKPHLHRIRIPALVLNARNDPFV
                     PAHCLPHPRDVGRFVTLWQPPHGGHVGFPGGRWPGHVNTLPEQVMDWIAKQI"
     gene            1659..2192
                     /locus_tag="VNU48_09600"
     CDS             1659..2192
                     /locus_tag="VNU48_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HWI11571.1"
                     /translation="MDDIVAQALKKWPNVPHCHGWLALDARGDWYLRDDAVQRAGPFP
                     RVKGSRIEHDKLRAFIERNYGHDAVGCWYFQNGPQRVYVELEAAPWVWRLSAVAGDAP
                     AIVSHTGAPARFESAWLDEAGRLFLAADLGFGVVHTLDMELAADAVEAQRWVPATARF
                     ETLPLRFGYQLSAAPPG"
     gene            complement(2264..>2960)
                     /locus_tag="VNU48_09605"
     CDS             complement(2264..>2960)
                     /locus_tag="VNU48_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWI11572.1"
                     /translation="AERIQPVGMVQVKDASDLSTLKTGEQVFAAQCTTCHTAGLVGAP
                     KFGDADAWAPRIKTGYDALLHSALAGKGNMGAQGGGDFTDLEVGRAVVYMANKAGAKF
                     DEPKAPAAAASAAPITAAAAPAAPATTAAPVAAAPTAVATAEPAKTDAGSAVPALYTQ
                     LCQTCHATGVANAPKLGDKAAWAPRIAEAKNIDGLTAIAIKGKGAMPPKGGSTASDAE
                     IKAVVTYMVGSVK"
BASE COUNT          411 a         1039 c         1052 g          458 t
ORIGIN      
        1 atcgagcagg acgtcgagcg cgtcgaccat tcgcgcgggc ctgaggactt caccgcggtc
       61 ggccgcgatg tcgaaagcgt cgtgctggcg cgcgcggtgc gctggcacgt cgagcaccga
      121 gtgctgctca acgggcgcaa gaccgttgtc ttcacatgac ggggcgcaag accgtcgtct
      181 tcacctgaga cctgctgccg atggcccgcc ccaagcccgt tgcgccgctc gcgtcccccg
      241 acgagatcga gcagcagttc tacgaggcgc tgcagcgcgg cgacctcgaa cgcctgatgg
      301 cggtgtggtc cgacgacgag gagatcagct gcgtgcaccc gggcgggccg cgcgtggtcg
      361 gcgccaccgc gatccgcagc gcgttcgaat cgatcttcgc gaacggcgcg gtcaacgcgc
      421 agcccgagaa ggtgcgccgg ctgcagaccc acgcctgctc ggtgcacagc gtgctcgagc
      481 acatccgcgt catgacgacc gaagggccgc agtcggcgtg ggtcgtcgcg accaacgtgt
      541 acctgttggg cgcacagggt tggcggctgg tctcgcacca cgcgagcccg ggcacgccgc
      601 gcgaggtgca ggagatcgtc gaggcggcct ccgtattgca ttgaacccga gccgcagatg
      661 cagcagtacc gtgcaccgcg ctggctgatc ggcggccatg cgcagaccat ctggccggcg
      721 ctgttctcga agcgtttcga cggcccggtg ccgagcttcc gccgcgagcg ctgggccacg
      781 ccggatggcg acttcatcga cgtcgactgg caggaggtgt ctgcccccgg gtcgccgggt
      841 acggcgaccg cgccgatgct cgtgttgttc cacggcctcg aaggttcgtc ggcgagccat
      901 tactcacagg cctttgcgca ctgggcgcgc gggcacggct ggcgcttcgt ggtcccacac
      961 ttccgcggtt gctcgggcga gctgaaccgc gcgccgcgcg cctaccactc gggcgacttc
     1021 gaggagatcg gctggatgct gcggcgcctg cgcgcgcaac atgaggggcg gatcgcggcg
     1081 gtcggcgtct cgctcggcgg caacgcgctg ctgcgctggg ccgaagaggc cggcgagagc
     1141 gccgcgcaga cggtcggcgc ggtcgcggcg gtgtcgtcgc cggtcgacct cgcggccggc
     1201 ggccatgcga tcggcatcgg cctcaaccgg caggtctaca cccgcatgtt cctgcgcacg
     1261 atgaagccga aggcgctggc caagctcgcc cagcacccgg ggctgttcga tcgcgacaag
     1321 ctgctcgccg cgcgcacgct gtacgagttc gacaacgtct tcaccgcgcc gctgcacggc
     1381 tttcgcgaca ccgacgacta ctggtcgcgt ggctcggcca agccgcacct gcatcgcatc
     1441 cgcatcccgg cgctggtgct gaatgcacgc aacgacccgt tcgtgccggc acactgcctg
     1501 ccgcatccgc gcgacgtggg gcgcttcgtc acgctgtggc agccgccgca cggcgggcac
     1561 gtgggcttcc ccggcgggcg ctggccgggg catgtcaaca ccttgcccga gcaggtcatg
     1621 gactggatcg cgaagcagat ctgagtcagg atcggctcat ggacgacatc gtcgcgcaag
     1681 cgctgaagaa gtggccgaac gtgccgcact gccatggctg gctggcgctc gacgcgcgcg
     1741 gcgactggta cctgcgcgac gatgccgtcc agcgtgcggg gccgttcccg cgggtcaagg
     1801 gcagccgcat cgagcacgac aagctgcgcg cgttcatcga acgcaactac gggcacgacg
     1861 ctgtgggctg ctggtacttc cagaacgggc cgcaacgcgt ctacgtcgaa ctcgaggcgg
     1921 cgccgtgggt ctggcggctg agcgcggtcg ctggtgacgc gccggcgatc gtcagccaca
     1981 ccggtgcccc tgcgcgtttc gagagcgcct ggctcgacga ggccggacgc ctgtttctcg
     2041 cggccgacct cggcttcggc gtggtccaca cgctggacat ggagctcgcc gccgacgccg
     2101 tcgaggcgca acgctgggtg cccgccaccg cgcgcttcga gaccttgccg ctgcgcttcg
     2161 gctatcaact cagcgccgcg ccgccgggtt gagacatcgg catggccggc gccaaaaaaa
     2221 gaagccggct cgcgagccgg cttccgtggc ggcgagtccc gcgttacttg acgctgccca
     2281 ccatgtaggt cacgaccgcc ttgatctcgg catcggacgc cgtcgagccg cccttcggcg
     2341 gcatcgcgcc cttgcccttg atggcgatcg cggtcaggcc gtcgatgttc ttggcctcgg
     2401 cgatgcgcgg cgcccaggcc gccttgtcgc ccagcttcgg cgcattggcg acgccggtgg
     2461 cgtggcaggt ctggcacagc tgcgtgtaca gcgccggcac cgcgctgccg gcatcggtct
     2521 tggcgggttc ggcggtggcg acggcagtcg gcgccgcagc aaccggcgcc gcggtcgtgg
     2581 cgggcgccgc aggtgccgca gcggcggtga tcggcgcagc cgacgctgcg gccgcaggcg
     2641 ccttcggttc gtcgaacttg gcgccggcct tgttcgccat gtagacgacg gcgcggccga
     2701 cttcgaggtc ggtgaaatca ccgccgccct gcgcgcccat gttgcccttg ccggccagcg
     2761 ccgagtgcag cagcgcgtcg tagccggtct tgatgcgcgg ggcccaggca tcggcgtcgc
     2821 cgaatttcgg tgcgccgacc aggccggccg tgtggcaggt cgtgcactgc gccgcaaaca
     2881 cctgctcgcc ggtcttcagg gtcgacaaat ccgaagcgtc cttcacctgc accatgccga
     2941 ccggctggat gcgctcggcc
//
LOCUS       DATMJB010000447         3090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_367511, whole genome shotgun sequence.
ACCESSION   DATMJB010000447 DATMJB010000000
VERSION     DATMJB010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367511"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="VNU48_09610"
     CDS             complement(<1..879)
                     /locus_tag="VNU48_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HWI11573.1"
                     /translation="MSPATDDAGDDLSALAWVHEELRRSLEAAHKALRRFVKETEALV
                     DADLDAVDPAVLRGARNQIHHGVGALELVGQPAAAMMLRASEALVQRFVTKPHKLSST
                     VVDDIERASFALLDYLGRLLAGKQVSTLSLFPQYRAVQEAAGADRMHPADLWPIDWQW
                     RELPADPSAHPLAHDAATQAAIEAQLLPLMRAAAPVQSAARMSDICAGLGAGATQPQI
                     ANLWKLAAAVFEAQAHGLLGFDVFSKRVASRLLAQYRIIARGDSDVSERLAQDLLFFC
                     AQSASPGDGRHSPRLAG"
     gene            complement(910..3018)
                     /locus_tag="VNU48_09615"
     CDS             complement(910..3018)
                     /locus_tag="VNU48_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HWI11574.1"
                     /translation="MADFSETRLQGDDLVAAGTGLPLIGRRPVVQQQRILFVMVGIGV
                     FGLILMTILSLLSANRGAAQVGASGQALMQSQRLAKSVSQALIGSVAAFPEVKDSADV
                     LVKNVRGLRIGDADIPAAPSGAQGAIEPMLPLIDRAEKSAKTVLDQQQVLTQVGQALR
                     AINKQSSDLLETAETVSSLKLQQDASPAELSAVGQLVMLTQRIGKSANEFLTMEGVSP
                     EAVFMLGKDLNTFRELGQGLLDGSPELRLPATKDAQTRERLVALMKQYEQTRTQASAI
                     LGNLQGLVSAREAQASIVADSEPLRQGLEAVQAQLGRETGFSATSLVLLLLSAVVLLL
                     GGTGLLRLYVRGQSERAAASEGQRVAAEREQQEAKRVNDANQAAILRLMNELQAVAEG
                     DLTQQATVTEDITGAIADSVNYTVEELRTLVSQVQGTVGRVTDTTQQVEATSTELLAA
                     SSEQLREIRDTGESVLQMAGRINDVSGHAQETAAVARQSLVAAESGLRAVQNTIGGMN
                     SIRDQIQETSKRIKRLGESSQEIGEITELISDITEQTNVLALNAAIQAASAGEAGRGF
                     SVVAEEVQRLAERSGDATRQIAALVKTIQTDTQDAVGAMERSTQGVVEGTRLSDAAGT
                     ALGEIDRVSRQLAELIEQISNQALSEARSANVVAANIQHIFAVTQQTGEGTRSTAQMV
                     RELSRTAEELRHSVARFKIG"
BASE COUNT          407 a         1111 c         1068 g          504 t
ORIGIN      
        1 cccggccagg cgcggcgaat gacggccgtc gcccggcgac gcgctctggg cgcagaagaa
       61 gagcaggtcc tgcgcgagac gctccgagac atcggagtcg ccgcgcgcaa tgatgcggta
      121 ctgcgccagc agccgcgagg ccacgcgctt gctgaacacg tcgaagccga gcaggccgtg
      181 cgcctgcgcc tcgaacaccg ccgccgcgag cttccacagg ttcgcgatct gcggctgcgt
      241 cgcgccggca ccgaggccgg cgcagatgtc gctcatgcgc gcggcgctct gcaccggtgc
      301 ggccgcgcgc atcagcggca gcagctgggc ctcgatcgcc gcctgcgtcg ccgcgtcgtg
      361 cgccagcggg tgggccgacg ggtcggccgg cagctcgcgc cattgccagt cgatcggcca
      421 gaggtcggcc gggtgcatgc ggtcggcgcc tgcggcctcc tgcaccgcgc ggtactgcgg
      481 gaacagcgac aaggtcgaga cctgcttgcc ggccagcagc cggcccaggt aatcgagcaa
      541 cgcgaacgac gcacgctcga tgtcgtcgac gaccgtcgag ctcagcttgt gcggcttcgt
      601 gacgaagcgc tgcaccagcg cctcgctggc gcgcagcatc atcgccgcgg cgggctggcc
      661 gaccagctcg agcgcaccca caccgtggtg gatctggttg cgggcgccgc gcagcacggc
      721 cgggtcgacg gcgtcgaggt cggcgtcgac cagcgcctcg gtctctttga cgaagcggcg
      781 cagcgccttg tgtgcagcct cgagcgagcg ccgcagttct tcgtgcaccc aggccagcgc
      841 gctgaggtcg tcgccggcgt cgtcggtggc ggggctcatg gcccgggcat caaaggggtt
      901 ggccacgact cacccgatct tgaaccgcgc gaccgagtgc ctcagctcct cggccgtgcg
      961 cgacagttcg cgcaccatct gcgcggtcga acgcgtgccc tcgccggtct gttgggtgac
     1021 cgcgaagatg tgctggatgt tcgcggccac gacgttggcg gaccgcgctt cgctcagcgc
     1081 ctggttcgag atctgctcga tcagctcggc gagctggcgc gacacgcggt cgatctcgcc
     1141 gagcgccgtg ccggcggcgt cggacagccg cgttccttcc accacgccct gcgtgctgcg
     1201 ttccatcgcg ccgaccgcgt cctgtgtgtc ggtctgaatc gtcttgacca gcgcggcgat
     1261 ctggcgcgtc gcgtcgcccg agcgttcggc caggcgctgc acttcttcgg ccaccaccga
     1321 gaagccgcga ccggcctcgc cggccgaggc cgcctggatc gccgcgttca gcgccagcac
     1381 gttggtctgc tcggtgatgt ccgagatcag ttcggtgatc tcgccgatct cctgagacga
     1441 ttcgcccagg cgcttgatgc gcttggaggt ttcctggatc tggtcgcgga tcgagttcat
     1501 gccgccgatc gtgttctgca ccgcacgcag gcccgactcg gccgcgacga gcgactggcg
     1561 cgccaccgcg gcggtctcct gcgcgtggcc cgacacgtcg ttgatgcgcc ccgccatctg
     1621 cagcaccgac tcgccggtgt cgcggatctc gcgcagttgt tcgctcgagg ccgcgagcag
     1681 ttcggtcgac gtggcttcga cctgctgcgt cgtgtcggtc acccggccca ccgtgccctg
     1741 cacctgcgac accagcgtgc gcagctcctc gacggtgtag ttcaccgagt cggcgatcgc
     1801 gccggtgatg tcctcggtga cggtcgcctg ctgcgtcaag tcgccttcgg ccaccgcctg
     1861 cagttcgttc atcagccgca gaatcgccgc ctggttcgcg tcgttgacgc gcttggcttc
     1921 ctgctgctcg cgttcggcgg cgacgcgctg gccttccgat gccgcggcgc gctcggactg
     1981 gccgcgcacg tacagccgca gcagcccggt accgccgagc agcagcacga ccgccgacag
     2041 cagcagcagc accaagctgg tggcgctgaa gccggtctcg cggccgagct gcgcttgcac
     2101 cgcctcgagg ccctgacgca gcggttcgct gtcggccacg atgctggcct gcgcctcgcg
     2161 cgccgacacc agcccctgca ggttgcccag gatcgcgctg gcctgggtgc gggtctgctc
     2221 gtactgcttc atcagcgcga ccaggcgctc tctggtctgc gcgtccttgg tcgcgggcag
     2281 gcgcaattcg gggctgccgt cgagcaggcc ctggccgagc tcgcggaagg tgttcaggtc
     2341 cttgccgagc atgaacacgg cctcggggct gacgccttcc atcgtcagga attcgtttgc
     2401 cgacttgccg atgcgctgcg tcagcatcac cagctggccg acggccgaca gctcggccgg
     2461 cgaagcgtcc tgctgcagct tcagcgacga cacggtctcg gcggtctcga gcaggtcgga
     2521 cgactgcttg ttgatcgcgc gcagcgcctg gcccacctgg gtcagcacct gctgctggtc
     2581 gagcacggtc ttcgcgctct tctcggcccg gtcgatcagc ggcagcatcg gctcgatcgc
     2641 gccctgcgcg cccgacggcg cggccgggat gtcggcatcg ccgatgcgca gcccgcgcac
     2701 gttcttgacc agcacgtcgg cgctgtcctt cacttccggg aacgcggcga cgctgccgat
     2761 cagagcctgc gacacagact tcgcgagccg ctgcgactgc atcagcgcct ggccgctggc
     2821 gccgacctgg gccgcaccgc ggttcgccga cagcagcgac aggatggtca tcaggatcag
     2881 gccgaacacg ccgatgccca ccatgacgaa caggatgcgc tgctgctgca cgaccggccg
     2941 gcggccgatc agcggcagcc cggtgccggc ggccacgagg tcgtcgccct gcaggcgggt
     3001 ctcggagaag tcggcgatct cggacggtgc ggcctccgag atgatcgagg tgtcgaagtc
     3061 ctggccctgc tgctgcgtcg gcacgtcgta
//
LOCUS       DATMJB010000448         2987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_862473, whole genome shotgun sequence.
ACCESSION   DATMJB010000448 DATMJB010000000
VERSION     DATMJB010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862473"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..116
                     /locus_tag="VNU48_09620"
     CDS             <1..116
                     /locus_tag="VNU48_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HWI11575.1"
                     /translation="LALPRICINGGRRGYLVGIAPQVLVDLLAARPVNCAL"
     gene            186..863
                     /gene="plsY"
                     /locus_tag="VNU48_09625"
     CDS             186..863
                     /gene="plsY"
                     /locus_tag="VNU48_09625"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019043340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HWI11576.1"
                     /translation="MQTLYPALATLVAYLIGSLSFAVIVSRAMGLRDPRSYGSGNPGA
                     TNVLRSGNQAAAVLTLALDALKGYLPVLLVKEFGAPYGLDEGTQAMVGLAAFLGHLWP
                     VFFHFKGGKGVATAAGVLFGLNPWLGLATLATWVIIAAFSRYSSLAAIVAALFAPFYQ
                     LLIWGGGPAAVAVMLMGGLLVWRHRANIQKLLKGTESRLGQKTAAPAVHAAKHATKHS
                     HKHGAKP"
     gene            860..1213
                     /locus_tag="VNU48_09630"
     CDS             860..1213
                     /locus_tag="VNU48_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWI11577.1"
                     /translation="MSQGLRRFHVGDRLSEMTIYNGTVYLAGQVASDASQDIRGQTTQ
                     VLAAIDKLLTEAGTDKAHILMCQIFIKDLADFPAMNAVWEDWLAPGDAPPRATVQANL
                     ARAEWKVEMVVTAAV"
     gene            1213..1581
                     /locus_tag="VNU48_09635"
     CDS             1213..1581
                     /locus_tag="VNU48_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HWI11578.1"
                     /translation="MATTARRPWLGLVVLVVVTAGAMQAFGWWSQQRLGAQVAAAARP
                     GDIQMIGSVTCVYCAQARAWFDENRVPFNECLIERDAACAERYNALMAPGTPVMFVRG
                     QRLLGFNAQAVADALATSVQ"
     gene            1613..2917
                     /locus_tag="VNU48_09640"
     CDS             1613..2917
                     /locus_tag="VNU48_09640"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWI11579.1"
                     /translation="MTQRVLTGITTTGTPHLGNYVGAIRPAIRASREPGVESFFFLAD
                     YHALIKCDEPARIERSRLEIAATWLAAGLDPSRVTFYRQSDIVEIPELTWLLTCVASK
                     GLMNRAHAYKAAADANLAAGEDVDAGITMGLFSYPILMAADILMFNAHRVPVGRDQVQ
                     HIEMARDLAQRFNHLFGAGRELFVLPEANVDERVAMLPGLDGRKMSKSYDNTVPLFEG
                     GAKGLRDAIARVVTDSRAPGEAKDPDGSHLVAIYEAFADDAERVALRSDLMAGMGWGD
                     AKARVVARIERDLGPLRERYAELIAHPERIEATLLAGARKARAVAAPFLAELRAAVGL
                     RPMVAVTAAAAVANKPKAALPSFKQYREADGRFYFKLAAADGRVLLQSAAFESGREAG
                     QWVAKLKSAGATAVPDAPVAHGEGVSAQDVADALAALAVAAG"
BASE COUNT          453 a         1046 c         1064 g          424 t
ORIGIN      
        1 cgctcgcgct gccgcgcatc tgcatcaacg gcgggcggcg cggctacctg gtgggcattg
       61 caccgcaggt gctggtcgac ctgctcgcgg ccaggccggt gaactgcgcg ctgtgaccgc
      121 gcgtcgccgc ggggcgcaaa atagcgccgc cccacttttc acggccccca ctccccgact
      181 gccccatgca gaccctgtac cccgcgctcg ccaccctcgt ggcctacctg atcggctcgc
      241 tgtcgttcgc ggtcatcgtg agccgcgcga tggggctgcg cgacccgcgc agctacggct
      301 cgggcaaccc gggcgcgacc aacgtgctgc gctcgggcaa ccaggccgcg gccgtgctga
      361 cgctggcgct cgatgcgctg aagggctacc tgccggtgct gctggtgaag gagttcggcg
      421 caccttacgg cctggacgaa ggcacgcagg cgatggtcgg cctggccgcc ttcctcggcc
      481 acctctggcc ggtgttcttc cacttcaagg gaggcaaggg cgtggccacg gcggcgggcg
      541 tgctgttcgg cctgaacccg tggctcgggc tggcgacgct ggccacctgg gtcatcatcg
      601 cggcgttctc gcgctactcg tcgctggcgg cgatcgtcgc ggcgctgttc gcgccgttct
      661 accagctgct gatctggggc ggaggccccg cggcagttgc ggtgatgctg atgggcgggc
      721 tgctggtgtg gcgccatcgc gcgaacatcc agaagctgct gaagggcacc gaaagcaggc
      781 tcggccagaa gaccgccgcc ccggccgttc atgcggccaa gcacgcgacc aaacattcac
      841 acaagcacgg agcgaaacca tgagccaggg actgcgtcga ttccatgtcg gcgaccgcct
      901 gtcggagatg acgatctaca acggcacggt ctacctcgcc gggcaggtcg cgagcgacgc
      961 ctcgcaggac atccgcggcc agaccaccca ggtgctggcg gcgatcgaca agctgctgac
     1021 cgaggccggc accgacaagg cgcacatcct gatgtgccag atcttcatca aggacctggc
     1081 cgacttcccg gcgatgaacg cggtctggga agactggctc gcgcccggcg acgcgccgcc
     1141 gcgcgcgacc gtgcaggcga atctcgcgag ggcggaatgg aaggtcgaga tggtggtcac
     1201 ggcggcggtg tgatggcaac gacggcgcgc cgaccctggc tcgggctggt cgtgctggtc
     1261 gtcgtcacgg ccggcgcgat gcaggccttc ggctggtggt cgcagcagcg cctgggcgcg
     1321 caggtggcgg ccgcggcgcg gcccggcgac atccagatga tcgggtcggt gacctgcgtc
     1381 tactgcgcgc aggcgcgcgc ctggttcgac gagaatcgcg tccccttcaa cgaatgcctg
     1441 atcgagcgcg acgcggcctg cgccgaacgc tacaacgccc tgatggcccc gggcacgccg
     1501 gtgatgttcg tgcgcggcca gcgtctgctc ggcttcaatg cgcaggccgt ggccgatgcg
     1561 ctcgcgacaa gcgtgcaatg acactgccac ttcgacaacg actcacgagc ccatgaccca
     1621 acgcgtcctc accggcatca cgaccaccgg caccccgcac ctcggcaact acgtcggcgc
     1681 gatccggccc gcgattcgcg cgagccgcga gcccggcgtc gagagcttct tcttcctcgc
     1741 cgactaccac gcgctgatca agtgcgatga gccggcgcgc atcgaacgct cgcgcctgga
     1801 gatcgccgcg acctggctcg ccgcggggct ggacccgtcg cgcgtcacgt tctaccggca
     1861 aagcgacatc gtcgagatcc ccgagctgac ctggctgctg acctgcgtgg cgtcgaaagg
     1921 cctgatgaac cgcgcgcacg cctacaaggc cgcggccgac gcgaacctgg cggccggcga
     1981 ggacgtcgac gccggcatca cgatgggcct gttcagctac ccgatcctga tggccgccga
     2041 catcctgatg ttcaacgcgc accgcgtgcc ggtgggccgc gaccaggtcc agcacatcga
     2101 gatggcgcgc gacctcgcgc agcgcttcaa ccacctgttc ggcgccggcc gcgagctgtt
     2161 cgtgctgccc gaggcgaacg tcgacgagcg cgtcgcgatg ctgccgggcc tggacgggcg
     2221 caagatgagc aagagctacg acaacaccgt gcccttgttc gaaggcggcg cgaagggcct
     2281 gcgcgacgcg atcgcgcgtg tcgtcaccga ttcgcgcgcg cccggcgagg cgaaggaccc
     2341 ggatggctcg cacctggtcg cgatctacga ggccttcgcc gacgacgccg agcgcgtcgc
     2401 actgcgttcc gacttgatgg ccggcatggg ctggggcgac gcaaaggcac gggtcgtcgc
     2461 gcgcatcgag cgcgatctgg ggccgctgcg cgaacgctat gccgaactga tcgcccaccc
     2521 cgagcgcatc gaggccacgc tgctcgcagg cgcacgcaag gcgcgcgcgg tggccgcgcc
     2581 gttcctcgcg gagttgcgcg cggcggtcgg gctgcggccg atggtcgcgg tgacggcggc
     2641 ggccgcggtc gcgaacaaac ccaaggcggc gctgccgagc ttcaagcaat accgcgaggc
     2701 cgacggcagg ttctacttca agctcgccgc ggccgacggc cgcgtgctgc tgcagagcgc
     2761 ggcgttcgag tcggggcgcg aggccgggca gtgggtcgcg aagctgaaga gcgcgggcgc
     2821 gaccgcggtg ccggacgcgc cggtcgcgca cggcgagggc gtcagcgcgc aggacgtggc
     2881 cgacgcgctc gcggcactgg cggttgccgc gggatgatcg ccgcgcggct caacgctcag
     2941 ggagatgcca tagatccgat ggttccgaaa cgcgcgggcc gaacgcg
//
LOCUS       DATMJB010000449         2498 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_420859, whole genome shotgun sequence.
ACCESSION   DATMJB010000449 DATMJB010000000
VERSION     DATMJB010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420859"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1479
                     /locus_tag="VNU48_09645"
     CDS             <1..1479
                     /locus_tag="VNU48_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HWI11580.1"
                     /translation="ATKGTTITRARLADHVQWDQGDRSWLLPLVDLEGPGERSGYFVP
                     LALAWEDHDEDRLKNLTLAALAKVRQQANIGVMGDAFSDEAFCRAMVAAIGAGLELPA
                     ASGRLHFRPTAAYADVAGPAVELAALPVSRPLAQSSNTIVMLGEKLFLKGYRRLRGGV
                     NPELEVGRHLTDVVHYPHCVPIAGALEYTAANGVVVSLALVQAFVANQGDGWSYTLAY
                     LERFLEPLRTAADAVPPDVHGAYLALVQTLGVRTAELHVALARATGDAAFDPEPLGTD
                     DFAALRQRAVAEAQASFALLRERVDQLADDAERDEARALLQQHEHVQERIAALAGAGG
                     GGGGVKIRYHGDYHLGQVLVTRNDFVIIDFEGEPARSFEERRAKGSALRDVAGMLRSF
                     NYALWSALRRVAHSGDELDRLAPHAQAWEQQTRTAFLAGYRRAVQGTPLAGAIEPGSG
                     LLGLFELEKALYELRYELGNRPDWVGVPLQGIQALLGDGGTS"
     gene            1500..2228
                     /locus_tag="VNU48_09650"
     CDS             1500..2228
                     /locus_tag="VNU48_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11581.1"
                     /translation="MQSVDMFLEPVRAFLSQVGSFLPRLVLALIVLVGGWLVAKLARF
                     AVVKALRTINFHVLTERSGMDGFMRQGGLQGDTSGIFGLLVYWLVILAALIIAFNGLG
                     LTYITDLLHRVVLFVPNVFVALIVLAFGGYFARFIGSTVIAYTRNVGLHDGELLGHLA
                     RYAILAFVVLIALDQVNIGGPVVRDSFLIVLGGIVFAVALAVGLGCKDWVARHLEERW
                     PSPGAAEQTPKDAGKPDLFDELGR"
     gene            complement(2253..2375)
                     /locus_tag="VNU48_09655"
     CDS             complement(2253..2375)
                     /locus_tag="VNU48_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11582.1"
                     /translation="MTDKHRHRHLNAPHGSAPNWVLTALGVCVVVAALLARSFI"
BASE COUNT          363 a          844 c          903 g          388 t
ORIGIN      
        1 gcgaccaagg gcaccaccat cacgcgcgcc cggctcgccg accacgtgca gtgggaccag
       61 ggcgaccgga gctggctgct gccgctggtc gatctcgaag gtccgggcga gcgctccggc
      121 tacttcgttc cgctggcgct ggcctgggaa gatcacgacg aggaccgcct gaagaacctg
      181 acgctcgcgg cgctggccaa ggtgcgccag caggcgaaca tcggcgtgat gggcgacgcg
      241 ttctccgacg aggcgttctg ccgcgccatg gtggcggcga tcggcgccgg gctcgagctg
      301 ccggcggcga gcgggcggct gcatttcagg ccgaccgcgg cgtatgccga cgtggccggc
      361 cccgccgtcg agctcgcggc gctgccggtg tcgcgcccgc tggcgcaaag cagcaacacg
      421 atcgtgatgc tcggcgagaa gctgttcctg aagggctacc ggcggctgcg cggcggcgtc
      481 aacccggaac tcgaggtcgg gcgtcacctg accgacgtgg tgcactatcc gcattgcgta
      541 ccgatcgccg gtgcgctgga gtacaccgca gccaacggtg tggtggtgtc gctggcgctg
      601 gtgcaggcct tcgtcgccaa ccagggtgat ggctggtcgt acacactggc ctacctcgag
      661 cgcttcctcg agccgctgcg caccgccgcc gacgcggtgc cacccgacgt gcacggcgcc
      721 tacctcgcgc tggtgcagac gctgggcgtg cgcaccgcgg agttgcatgt ggcgctcgcg
      781 cgcgcgaccg gcgacgcggc gttcgatccc gagccgctgg gcaccgacga cttcgccgcg
      841 ctgcgccagc gtgccgtcgc cgaagcccag gcgagcttcg cgctgctgcg ggaacgggtc
      901 gaccagctgg ccgacgacgc cgagcgcgac gaggcgcgcg ccctgctgca gcaacacgag
      961 cacgtgcagg agcgcatcgc cgcgctggcg ggcgcgggcg ggggcggggg cggcgtgaag
     1021 atccgctacc acggcgacta ccacctcggc caggtgctgg tcacccgcaa cgatttcgtc
     1081 atcatcgact tcgagggcga gccggcgcgc agcttcgaag aacggcgcgc gaagggctcg
     1141 gcgctgcgcg acgtggccgg catgttgcgc tcgttcaact atgcgctctg gagcgcactg
     1201 cgccgggtcg cacacagcgg cgacgaactc gaccggctgg cgccgcacgc gcaggcctgg
     1261 gagcagcaga ccagaaccgc cttcctggcc ggctaccggc gcgcggtgca gggcacgccg
     1321 ctggcgggag cgatcgaacc cggaagcggc ctgctcggcc tgttcgagct cgagaaggca
     1381 ctctacgaac tgcgctacga actcggcaac cgccccgact gggtcggcgt gccgctgcag
     1441 gggatccagg cgctgctcgg cgacggcggc acgagctgat cacatcaagg agggcccaga
     1501 tgcaaagcgt cgacatgttc ctcgaaccgg tgcgggcgtt cctgtcgcag gtggggtcct
     1561 tcctgccgcg gctggtgctg gcgctgatcg tgctggtcgg tggttggctg gtggcgaagc
     1621 tggcgcgctt cgcggtggtc aaggcgctgc gcacgatcaa cttccacgtg ctgaccgagc
     1681 gctcgggcat ggacggcttc atgcgccagg gcggcttgca gggcgacacc agcgggatct
     1741 tcgggctgct ggtgtactgg ctggtgatcc tggccgcgct catcatcgcc ttcaacggcc
     1801 tcgggctcac gtacatcacc gacctgctgc atcgggtggt gctgttcgtg ccgaacgtgt
     1861 tcgtggcgct gatcgtgctc gcgttcggcg gctatttcgc gcgcttcatc ggcagcaccg
     1921 tcatcgccta cacgcgcaac gtcggcctgc acgatggcga gttgctgggc cacctcgccc
     1981 gttacgcgat cctcgccttc gtcgtgctga tcgcgctcga ccaggtcaac atcggcgggc
     2041 cggtcgtgcg cgacagtttc ctgatcgtgc tcggcgggat cgtgttcgcc gtggcgctgg
     2101 ccgtcgggct gggctgcaag gactgggtcg cccgccacct cgaagaacgc tggccgagcc
     2161 ccggggcggc ggagcagacg ccgaaggacg ccggcaagcc ggacctgttc gacgagttgg
     2221 gtcgctgagc gctccggcgc ggcgagaccg tttcagatga acgatcgcgc cagcagcgcg
     2281 gcgacgacga cgcagacgcc caacgccgtc aggacccagt tgggcgctga tccgtgcggc
     2341 gcgttgaggt ggcgatgacg atgtttgtcg gtcatgtgag tttcagctgc gagtggagcg
     2401 gtcagaaatg gcgccccgac gctcacgccg ggagcctgac cacacacgca cacacccaaa
     2461 gcgatcttcg tgccagcgtc gtcgcacccg cttcagtc
//
LOCUS       DATMJB010000450         2969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_420476, whole genome shotgun sequence.
ACCESSION   DATMJB010000450 DATMJB010000000
VERSION     DATMJB010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420476"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2969)
                     /locus_tag="VNU48_09660"
     CDS             complement(<1..>2969)
                     /locus_tag="VNU48_09660"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11583.1"
                     /translation="ALSTQYAYDARGNLVRETNADGIAATTRFDALDRAIVTTDGEGF
                     SVSFAYDRFGNQTAITTGQYLVAVGDAGYDAAKAARAMPATTRIAYDAMDRKLLQADA
                     LGTVTRYAYDARGNRTLQTEAYGQLASGQALTEGSIGVFGAGVQRISRFAFDALDRLT
                     DETQPIGTVVHHDYNGAGELSAKIVDYGSGAQFRNATTRYFHDAGGRLSFEADPIGSV
                     TRYQYDDFGNRVRTVRGLALDAQGQPSDALTADARVTAQEYDAAHRLTAEVVDPDGLA
                     LRTAYEYDARDNRVAVVDANGARSEIGYDAADRAVWARDAEGYVTRADYDGRGLRIAE
                     TRYATRASSLAAGAVPAASTSDRVSRFAYDGAGRQVEATDARGVVSRMSFDAIGNLLQ
                     RAENATALYGSAPRITSYTYTLANQVATQTDASGLVTRFSYDAVYNLAEKRVENRWLD
                     TLNLDANGAPTERVEVQVTANVYDLNNRLTDTVTDPGGLNLHLGYRYDSLGNRIAEIA
                     ANGYAAADTDSAAALSARRALGLVDAAGQPLAAAQLTAAQRQGVLDAYSTRTWFDAAG
                     RAVLSVDANGHAKTAQYDAVGNLVGSTQYAQPLSTAQVAALDALTLPQLTAAGSDRTL
                     NWVFDKADRLVLERGAAARQYVNGAWQDDHRAETSKRYDGVGNLIGQTDANGNSSYLY
                     YDANRRLQGRIDAEGFLTVNRLDAFGDVVEERLSLTRPDLSASQKAALDLDTYVPAGE
                     ARVIAHQYDALGLELRTLYPAADLYENGAESSAQVQVLRSFDASGAVLSETVKHRVGE
                     TAPAATHYQYDAAGRLVGKTDARADELTGSDAAEIVALRADLGYVDSTGNGKPASQLS
                     GAEKAALVASYFSGYAYDAVGNVREQVEGARVTTFEYDRANRNLRVHYPATRRVEVDA
                     NGTITTTENYRALGQRAYDASGNVIAETKPNGERIDYRFDRGNRQVAALNDGVYVEYG
                     YNFAGDTTLIHRYFAAA"
BASE COUNT          329 a          956 c         1164 g          520 t
ORIGIN      
        1 ctcgccgcgg cgaagtagcg gtggatcagc gtcgtgtcgc cggcgaagtt gtagccgtat
       61 tcgacataga cgccgtcgtt cagcgccgcg acctggcggt tgccgcggtc gaagcggtag
      121 tcgatgcgct cgccgttggg cttggtctcg gcgatcacgt tgcccgaggc gtcgtaggcg
      181 cgctggccca gcgcgcggta gttctcggtg gtggtgatgg tgccgttcgc gtcgacctcg
      241 acacgacggg tcgccgggta gtgcacgcgc aggttgcggt tggcgcggtc gtactcgaag
      301 gtggtgacgc gcgcgccctc gacctgctcg cgcacgttgc ccacggcgtc gtaggcgtag
      361 ccgctgaagt agctcgccac cagcgcggcc ttctcggcgc cgctgagctg gctcgccggc
      421 ttgccgttgc cggtcgagtc gacatagccc aggtcggcgc gcagcgcgac gatctcggcc
      481 gcgtcgctgc ccgtcagctc atcggcgcgg gcgtcggtct tgccgaccag gcggccggcg
      541 gcgtcgtact ggtagtgcgt cgcggccggc gcggtttcgc cgacgcggtg cttcacggtc
      601 tcgctgagca cggcgccgct ggcgtcgaag ctgcgcagga cctgcacctg ggcgctgctc
      661 tcggcgccgt tttcgtacag gtcggccgcc gggtacagcg tgcgcagctc gaggccgagc
      721 gcgtcgtact ggtgggcgat cacgcgggcc tcgccggccg gcacgtaggt gtcgaggtcg
      781 agcgcggcct tctgcgatgc cgacaggtcg ggccgggtca ggctcaggcg ctcttcgacg
      841 acatcgccga acgcgtcgag ccggttgacc gtcaggaagc cttcggcatc gatgcggccc
      901 tgcagcctgc ggttggcgtc gtagtacagg tagctgctgt tgccgttggc atcggtctgg
      961 ccgatcaggt tgcccacgcc gtcgtaacgc ttgctggtct cggcgcggtg gtcgtcctgc
     1021 caggcgccgt tgacgtactg gcgcgccgcg gcgccgcgtt cgagcacgag ccggtcggcc
     1081 ttgtcgaaca cccagttcag cgtgcggtcg ctgccggcag cggtgagctg cggcagcgtc
     1141 agcgcgtcga gcgccgccac ctgcgcggtg ctcagcggct gcgcgtactg ggtgctgccg
     1201 acgaggttgc cgactgcgtc gtattgcgcg gtcttggcat ggccgttggc atcgacgctg
     1261 agcacggcgc ggccggcggc atcgaaccag gtgcgggtgc tgtaggcatc gagcacgccc
     1321 tggcgctggg cggccgtgag ttgcgccgcg gcgagcggct ggcccgcggc gtcgaccagg
     1381 ccgagcgcgc gccgcgccga cagcgctgcg gcgctgtcgg tatcggcggc ggcgtagccg
     1441 ttggcggcga tctcggcgat gcggttgccc aggctgtcgt agcggtagcc caggtgcagg
     1501 ttcaggccgc cggggtcggt gaccgtgtcg gtgaggcggt tgttgaggtc gtagacgttg
     1561 gcggtgacct gcacctcgac gcgctcggtc ggcgcgccgt tggcgtcgag gttcagggtg
     1621 tcgagccagc ggttctcgac gcgcttctcg gcgaggttgt agaccgcgtc gtagctgaag
     1681 cgcgtgacca ggccgctggc gtcagtctgc gtcgccacct ggttggcgag cgtgtaggtg
     1741 tagctggtga tgcgcggcgc gctgccgtac agcgcggtcg cgttctcggc ccgctgcagc
     1801 aggttgccga tcgcatcgaa gctcatgcgg ctgacgacgc cgcgcgcatc ggtggcttcg
     1861 acctggcggc cggcgccatc gtaggcaaag cggctgacgc ggtcgctcgt gctggcggcc
     1921 ggcacggcgc cggccgcgag gctcgaggcg cgggtcgcgt aacgggtctc ggcgatgcgc
     1981 aggccgcggc cgtcgtagtc ggcgcgggtt acgtagcctt cggcgtcgcg cgcccacacg
     2041 gcgcggtcgg cggcgtcgta gccgatctcg ctgcgggcgc cgttggcgtc gacgacggca
     2101 acccggttgt cgcgggcgtc gtactcgtag gcggtgcgca gtgccagccc gtcggggtcg
     2161 acgacctcgg cggtcaggcg atgggcggcg tcgtactcct gcgcggtcac gcgcgcgtcg
     2221 gcggtgagcg cgtcggacgg ctgcccttgc gcgtcgagcg cgaggccgcg aacggtgcgc
     2281 acgcggttgc cgaaatcgtc gtattggtac cgggtgacgc tgccgatcgg gtcggcctcg
     2341 aagctgaggc ggccgccggc atcgtggaag tagcgcgtcg tcgcgttgcg gaactgcgca
     2401 ccgctgccgt agtcgacgat cttggcgctg agttcgccgg cgccgttgta gtcgtggtgg
     2461 accacggtcc cgatcggctg ggtttcgtcg gtcaggcggt cgagcgcgtc gaaggcgaag
     2521 cggctgatgc gttgcacgcc ggcgccgaaa acgccgatgc tgccctcggt gagcgcctgg
     2581 ccgctcgcga gctggccata ggcctcggtc tgcagcgtgc ggttgccgcg cgcgtcgtag
     2641 gcgtagcggg tgacggtgcc cagcgcatcg gcttgcagca gcttgcggtc catcgcgtcg
     2701 tacgcgatcc gggtcgtggc cggcatggcg cgcgcggcct tcgcggcgtc gtagccggca
     2761 tcgcccaccg cgacgaggta ttgcccggtc gtgatcgcgg tctggttgcc gaaccggtcg
     2821 taggcgaacg agacgctgaa gccttcgccg tcggtcgtga cgatcgcgcg gtccagcgcg
     2881 tcgaagcggg tggtggcggc gatgccatcg gcgttggtct cgcgcaccag gttgccgcgc
     2941 gcgtcgtagg cgtactgggt gctcagcgc
//
LOCUS       DATMJB010000451         2246 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_626548, whole genome shotgun sequence.
ACCESSION   DATMJB010000451 DATMJB010000000
VERSION     DATMJB010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2246
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626548"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VNU48_09665"
     CDS             complement(<1..689)
                     /locus_tag="VNU48_09665"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWI11584.1"
                     /translation="MSAWPVLTLVTFLPLVGAAFILVVRGDEQTVASNARWVALWTTL
                     VTFVVSLALWFDFDRGTAAFQFVERAPWFPGTTISYHMGVDGVSVLFVLLSTFLMPIC
                     ILASWEAIEYRVREYMIAFLVLETMMVGTFCALDLVLFYVFFEGVLIPMFLIIGIWGH
                     GRRVYSAFKFFLYTLAGSVLMLIALMAMYWSAGTTDIPTLLRHAFSPRMQTWIWLAFF
                     ASFAVKVPMWPV"
     gene            complement(700..>2246)
                     /gene="nuoL"
                     /locus_tag="VNU48_09670"
     CDS             complement(700..>2246)
                     /gene="nuoL"
                     /locus_tag="VNU48_09670"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWI11585.1"
                     /translation="LVQMFFGWEGVGLASYLLIGFWYKRPSANAAAIKAFVVNRIGDF
                     GFALGIFGTFFLFGTVQFEAVFAAVPGHAGDTMRFLWFEGHALTILCLLLFVGAMGKS
                     AQLGLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVARLSPMFEYAPAALATVTIIGA
                     LTAIFAASVGLVQNDIKRVIAYSTCSQLGYMFFAAGVSAYSAAIFHLMTHAFFKALLF
                     LGAGSVIHALSDEQDLRKMGGLARIIPKTTAVMWIGSLALAGIGIPHLVGFAGFYSKD
                     TILEAAFGAHSAAGQFAYWLGVAAAGMTAFYSWRLLYLAFHDGSRVDHHALEHAHESP
                     NVMLIPLYILSFGAVFAGAIGFHFFVGEGREEFWRESIKVLHEHDSIEAAHHVSFWVG
                     TLPLVVSVAGIGLAWRMYIVDPALPARLAARFRGVYEFLLNKWWFDELYDAIIVRPVF
                     RLGLGFWKKGDEAVIDGLGADALAANTISAARRVAALQTGYVYHYAFAMLVGVVALAS
                     WYALTR"
BASE COUNT          447 a          741 c          784 g          274 t
ORIGIN      
        1 accggccaca tcggcacctt gaccgcgaac gaggcgaaga aggcgagcca gatccaggtc
       61 tgcatgcgcg ggctgaaggc gtggcgcagc agcgtcggga tgtcggtcgt ccccgcgctc
      121 cagtacatcg ccatcagcgc gatcagcatc agcaccgatc cggcgagcgt gtagaggaag
      181 aacttgaacg ccgagtagac gcgccgcccg tggccccaga tgccgatgat caggaacatc
      241 gggatcagca cgccctcgaa gaagacgtag aacagcacga ggtcgagcgc gcagaacgtg
      301 ccgaccatca tcgtctcgag cacgaggaag gcgatcatgt actcgcgcac gcggtactcg
      361 atcgcctccc acgaggcgag gatgcagatc ggcatcagga aggtggacag cagcacgaac
      421 agtaccgaca cgccgtcgac gcccatgtgg tagctgatcg tcgtccccgg gaaccacggc
      481 gcccgctcga cgaactggaa tgcggcggtg ccgcggtcga agtcgaacca cagcgccagc
      541 gagacgacga aggtcaccag cgtcgtccac agcgccaccc agcgcgcgtt ggaggcgacc
      601 gtctgctcgt cgccgcgcac gaccaggatg aacgccgcgc cgaccagcgg caggaaggtg
      661 acgagcgtga ggacgggcca ggcggacatc ggcggcgcct cagcgggtca gcgcgtacca
      721 gctggcgagg gcgaccaccc cgaccagcat ggcgaacgcg tagtggtaga cgtagccggt
      781 ctgcagcgcg gccacccgcc gcgccgcgga gatcgtgttg gcggccagcg catcggcgcc
      841 gagcccgtcg atcaccgcct cgtcgccctt cttccagaag ccgaggccta ggcggaagac
      901 cggccggacg atgatcgcgt cgtagagctc gtcgaaccac cacttgttga gcaggaactc
      961 gtagacgccg cggaagcgcg cggcgagccg cgccggcagc gccggatcga cgatgtacat
     1021 cctccaggcg aggccgatcc cggcgacgct caccaccagc ggcagcgtgc cgacccagaa
     1081 cgagacgtgg tgcgcggcct cgatgctgtc gtgctcgtgg agcaccttga tcgactcgcg
     1141 ccagaactcc tcgcggccct cgccgacgaa gaagtggaag ccgatcgcgc cggcgaacac
     1201 cgcgccgaag gagaggatgt agagcgggat cagcatcacg ttgggcgatt cgtgcgcgtg
     1261 ctcgagcgcg tggtggtcga cgcggctgcc gtcgtggaac gccaggtaga gcaggcgcca
     1321 ggagtagaac gcggtcatcc ccgcggcggc gacgccgagc cagtaggcga actgcccggc
     1381 cgccgaatgg gcgccgaagg ccgcctcgag gatggtgtcc ttggaataga agccggcgaa
     1441 gccgacgagg tgcgggatgc cgattccggc gagcgcgagc gagccgatcc acatcaccgc
     1501 ggtggtcttg gggatgatgc gcgcgagccc gcccatcttg cgcaggtcct gctcgtccga
     1561 cagcgcgtgg atcaccgagc cggcgccgag gaagagcagc gccttgaaga aggcgtgcgt
     1621 catcaggtgg aagatcgccg ccgagtaggc cgagacgccg gcggcgaaga acatgtagcc
     1681 gagctgcgag caggtcgagt aggcgatcac ccgcttgatg tcgttctgca ccagcccgac
     1741 cgaggccgcg aagatcgcgg tcagcgcgcc gatgatcgtc accgtcgcca gcgccgcggg
     1801 cgcgtactcg aacatcggcg acagccgcgc caccatgaac acgcccgcgg tcaccatcgt
     1861 cgcggcgtgg atcagcgccg acaccggcgt cgggccctcc atcgcgtcgg gcagccaggt
     1921 gtgcagcccg agctgcgccg acttgcccat cgcgccgacg aacagcagca ggcacaggat
     1981 cgtcagcgcg tggccctcga accagaggaa gcgcatcgtg tcgccggcgt ggccgggcac
     2041 ggcggcgaac accgcctcga actgcacggt gccgaacagg aagaaggtgc cgaagatgcc
     2101 gagcgcgaag ccgaaatcgc cgatgcggtt gaccacgaaa gccttgatcg cggcggcgtt
     2161 ggcggagggg cgcttgtacc agaagccgat caagaggtag ctcgcaagcc cgacgccttc
     2221 ccagccgaag aacatctgca cgaggt
//
LOCUS       DATMJB010000452         2049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_418219, whole genome shotgun sequence.
ACCESSION   DATMJB010000452 DATMJB010000000
VERSION     DATMJB010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418219"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VNU48_09675"
     CDS             <1..213
                     /locus_tag="VNU48_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11586.1"
                     /translation="AEAVKAHAATTPVILLTGWGQRLVADGEVPAHVDRVLSKPPKLR
                     ELRAALARLTRRAERGADDESTQEEA"
     gene            210..1358
                     /locus_tag="VNU48_09680"
     CDS             210..1358
                     /locus_tag="VNU48_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI11587.1"
                     /translation="MIEPAGARLLIVDDEVAQMQALCDTLEEQGYEVVGCTNGEAALA
                     AMRRAPVDLLLADLMMPGVNGIDLLRAALAIDPMLVGVIMTGEGTIGTAVEAMKSGAL
                     DYILKPFKLSAILPVLGRGLAMRRLRLEKAALERRVQEHAVELETMNRELDAFTRSAS
                     HDLRSPLNVVLGFSSLLVSNIGPTMPIEQRNWLLQIERAARQMSDLIDALMRLSFVGK
                     QALNLQVVEVAALVHSVLDELRDGHPQRSVDVRVGELPEVMADPLLLRQVFVNLLSNA
                     YKYTREVEPGEIEVACEQQGDEPVFRVGDNGSGFDMAHADRLFQAFQRFHHGDQFEGS
                     GVGLSIVQRIVKRHGGRIWAQAAPGKGASFFFTLGATSTPKRGANGER"
     gene            complement(1394..>2049)
                     /locus_tag="VNU48_09685"
     CDS             complement(1394..>2049)
                     /locus_tag="VNU48_09685"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWI11588.1"
                     /translation="RTVGEGGEVLGVDVSRPMLDVARAQAGLAGRARLAFLEADASEA
                     ALPAGVDLLFSRFGVMFFGQPVPAFRHLRGSLRSHGRFVFVCWRAPRDNSWAMTPLAA
                     ARQAMNITPVPVDPNAPGPFAFADDERLRAILSDAGFENTDIRRFDAPIHLGATPRAA
                     AENAARIGPASRFVRDVGIENLPAIVDAIENALQPLAASDGRVSLPGSTWLVVASNP"
BASE COUNT          340 a          666 c          747 g          296 t
ORIGIN      
        1 gccgaagccg tcaaggcgca tgctgcgacg acgccggtga tcctgctcac cggctggggg
       61 cagcgcctgg tggccgacgg cgaggttccg gcgcacgtcg atcgggtgct gagcaagcca
      121 cccaagctgc gcgagttgcg cgccgcgctg gcccgcttga cacggcgcgc cgaacggggt
      181 gccgacgacg aatcgacaca ggaggaagca tgatcgaacc cgccggcgca cgattgctca
      241 tcgtcgacga cgaagtggcg cagatgcagg ccttgtgcga cacgctggag gagcagggct
      301 acgaggtcgt cggctgcacc aacggcgagg ccgcgctggc cgccatgcgc cgcgcgccgg
      361 tcgacctgct gctggccgac ctgatgatgc ccggcgtgaa cggcatcgat ctgttgcgtg
      421 cggcgctggc gatcgacccg atgctggtcg gcgtcatcat gaccggcgag ggaacgatag
      481 gcaccgcggt cgaggcgatg aagtccgggg cgctggacta catcctcaag ccgttcaagc
      541 tgagcgcgat cctgccggtg ctcggccgcg gcctggcgat gcggcgcctg cgcctggaga
      601 aggcggcgct cgagcggcgc gtgcaggagc acgcggtcga actcgagacg atgaaccgcg
      661 aactcgatgc cttcacccgg tcggcgtcgc acgatctgcg ctcgccgctg aacgtggtgc
      721 tggggttctc atcgctgctg gtcagcaaca tcggcccgac gatgccgatc gaacagcgca
      781 actggctgct ccagatcgag cgcgcggccc gccagatgtc ggacctgatc gacgcgctga
      841 tgcgcctgtc gttcgtgggc aagcaggcct tgaacctgca ggtcgtggag gtcgcggcgt
      901 tggtgcacag cgtcctcgac gagttgcgcg acgggcaccc gcagcgcagc gtcgacgtgc
      961 gggtcggcga actgcccgag gtgatggcag acccgttgtt gctgcgccag gtgttcgtca
     1021 acctgctgtc gaacgcctac aagtacacac gcgaggtcga gccgggcgag atcgaggtgg
     1081 cctgcgagca gcagggagac gagcccgtct tccgggtcgg ggacaacggc tcgggcttcg
     1141 acatggccca cgccgaccgg ttgttccagg cgttccagcg cttccatcat ggcgaccagt
     1201 tcgagggcag cggcgtcggg ctgtccatcg tgcagcgcat cgtgaagcgt cacggcgggc
     1261 gcatctgggc gcaggccgcg ccgggaaagg gcgcgagctt cttcttcacg ctcggagcga
     1321 catccactcc gaagcgtggt gcgaacggcg agcggtgagc tcgagtgcgg cggcagagta
     1381 gcgctcgctg gcactacgga tttgaggcga ccacgagcca tgtagagccg ggcaggctca
     1441 cccgaccatc ggaggctgcc agcggctgga gggcgttttc gatggcatcg acgatcgcgg
     1501 gcaggttctc gatgcccacg tcacgcacga agcgcgaggc cgggccgatg cgcgccgcgt
     1561 tctcggcagc ggccctcggc gtcgcgccca aatggatcgg tgcatcgaag cgccgaatat
     1621 cggtgttctc gaagccggcg tccgacagga tggcgcgcaa tcgttcgtcg tccgcaaacg
     1681 cgaaggggcc gggcgcgttc ggatcgaccg gcaccggtgt gatgttcatg gcctggcggg
     1741 cggcggcgag cggcgtcatg gcccagctgt tgtcgcgcgg ggctcgccag cacacgaaga
     1801 cgaagcggcc gtgcgatcgc agcgagcctc tcaaatggcg gaaggcgggc accggctggc
     1861 cgaagaacat caccccgaag cgcgagaaca acagatccac gccggcgggc agtgccgcct
     1921 cggacgcgtc ggcctccaga aacgcgagcc tggcccggcc cgccagcccg gcttgggccc
     1981 gcgcgacgtc gagcatgggt cgcgagacgt cgacacccag aacctcgccg ccttcgccaa
     2041 ccgtgcgag
//
LOCUS       DATMJB010000453         4042 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_486596, whole genome shotgun sequence.
ACCESSION   DATMJB010000453 DATMJB010000000
VERSION     DATMJB010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486596"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1303)
                     /gene="gspL"
                     /locus_tag="VNU48_09690"
     CDS             complement(38..1303)
                     /gene="gspL"
                     /locus_tag="VNU48_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HWI11589.1"
                     /translation="MSTLVVQIPPRQRLRSSAGSAEPEASGLGTEYAYVTSPDGLALE
                     AQGRCAASLFPKATTVIAVLADTDVSWHRITLPKAPGARLRAALGGVLEEALLEDAED
                     VHLALAPAAVAGQPTWVAAVSRRWLRAELAALEKAEVFVDRVVPMAWPDDPPIGHFSE
                     TTSDPGGNTHGIALHWAHADGVATVRLQGGLARALVPVPAPPETRWSATPGAVAAAEQ
                     WLGGPVRVMPPGQRLLQAARSLWNLRQFELARRNRGVRALGDSARKFFSPEWRPVRWG
                     LAALVLAQIAGLNLWAWHQRSAIEARQLAMQTIVRTTYPNANPQDIQRDADAVMQRET
                     QALRTLAGKPGDTDLEPMLQAAASAWPADAAAVESIRFEPGRLTLSANGWSDAQIAQF
                     RSLLRPNGWSVDAAEGRLTLSRARPGASS"
     gene            complement(1419..2396)
                     /gene="gspK"
                     /locus_tag="VNU48_09695"
     CDS             complement(1419..2396)
                     /gene="gspK"
                     /locus_tag="VNU48_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HWI11590.1"
                     /translation="MPAHRLTRHRPSRQRGAALLTAMIIVTLVATLATAMVWQQWRAV
                     QVESAERARAQAAWILSGALELAKLILREDQKSGRPTALTEPWATPLAESRLSTFLAV
                     DKANADDGPEAFLSGSISDAQARYNLTNLVTLATSAAPAQINRPELQTLERLCERVGV
                     ETGVAQRIATGLLAAMTASSGAPLPPKTTAQLSWLGIDPAAIQALQPYVVLLPDPTPL
                     NVNTASKEVLGAVINTDQASAERLVQIGQRTPFKDVPAFKAQSASPSATAASAPPNIG
                     VHSDFFEVRGRLRLMDRVLVERSLVQRLARGQVNVLQRERIAGLDQPGS"
     gene            complement(2403..3062)
                     /locus_tag="VNU48_09700"
     CDS             complement(2403..3062)
                     /locus_tag="VNU48_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWI11591.1"
                     /translation="MNRFARQGGFTLVEVLVAMVVMAIMAVMAWQGVDGIVRTRDASQ
                     ARLEQTLRLNTVIAQWDQDLASIQDSGAVPESLKCDGASVRMVRRTPDGLQVVAWSYR
                     PDDSGGAWVRWAGPSVTTTSALQDSWLRSLQLQGGEPGSLKALTGVAGWQVYFFQTNG
                     WANCQSTGNVVVAAAAGASAVPGPLTTRTVLPDGVRVVLSFVPGSGINGDLTRDTLRG
                     P"
     gene            complement(3059..3421)
                     /gene="gspI"
                     /locus_tag="VNU48_09705"
     CDS             complement(3059..3421)
                     /gene="gspI"
                     /locus_tag="VNU48_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HWI11592.1"
                     /translation="MSTRRAPGFTLIEVMVALAIVAITLGAGIKAAGALTGNTQRLAD
                     ATAAQWCADNQLTGLRLSKLTPPIGDSEFSCVELGQAYAGRLSVRPTPNPLFVNVDAA
                     IADENGQPILNLTTVLRK"
     gene            complement(3421..3882)
                     /locus_tag="VNU48_09710"
     CDS             complement(3421..3882)
                     /locus_tag="VNU48_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWI11593.1"
                     /translation="MNARRAGFTLIELMVVVALIAIASAVASLALRDPASTRLEHEGA
                     RLVALLESARSEARAAGLAASWEPRAAQEGAEGFRFVGLPPSSELPTQWLVPGVTAEV
                     LGARAVVLGPEPLIGAQRVVLHLESQSLTLATDGLGPFTVIDGEPAAVPRS"
     gene            complement(3893..>4042)
                     /locus_tag="VNU48_09715"
     CDS             complement(3893..>4042)
                     /locus_tag="VNU48_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspG"
                     /protein_id="HWI11594.1"
                     /translation="YLDKLPNDPWGHPYQYANPGLKGEIDVYSFGADGVVGGEGKNAD
                     LGSWQ"
BASE COUNT          551 a         1418 c         1476 g          597 t
ORIGIN      
        1 gccagcgccg gcgaggcggt ggccaggttg gtcgcggtca cgagctcgct cccggccgcg
       61 cgcggctcag ggtcaggcgc ccttcggcgg catcgaccga ccagccgttc gggcgcagca
      121 ggctgcggaa ctgggcgatc tgggcgtcgc tccagccgtt cgccgacaag gtcagtcggc
      181 cgggctcgaa gcggatcgac tcgacggccg ccgcgtcggc cggccaggcc gacgcggcgg
      241 cctgcagcat cggctcgagg tcggtgtcgc cgggcttgcc ggcgagcgtg cgcagggcct
      301 gcgtttcgcg ctgcatcacg gcgtcggcgt cgcgctggat gtcttgcggg ttcgcgttcg
      361 ggtaggtcgt cctgacgatg gtctgcatcg ccagctggcg cgcctcgatc gcgctgcgct
      421 ggtgccaggc ccacaggttc aggccggcga tctgcgccag cacgagtgcc gcgaggcccc
      481 agcgcaccgg gcgccactcg gggctgaaga acttgcgcgc cgagtcgccg agcgcgcgca
      541 cgccccggtt gcggcgcgcg agttcgaact ggcgcaggtt ccacaggctg cgcgcggcct
      601 gcagcaggcg ctggccgggc ggcatcacgc gcaccgggcc gcccagccac tgctcggcgg
      661 cggcgacggc gccgggcgtg gcgctccagc gcgtttcggg cggggccggc accggcacga
      721 gggcacgggc caggccgccc tgcagccgca cggtggccac gccgtcggcg tgggcccagt
      781 gcagtgcgat gccgtgcgtg ttgccgccgg ggtcggaggt ggtctcggag aagtggccga
      841 tcggcgggtc gtccggccag gccatcggca cgacgcggtc gacgaacacc tcggccttct
      901 cgagcgccgc gagttcggcg cgcagccagc gccgcgagac cgccgcgacc caggtcggct
      961 ggccggcgac ggcggccggc gcgagggcga ggtgcacatc ctcggcgtcc tcgagcaggg
     1021 cttcttcgag cacgccgccg agcgcggcgc gcagccgcgc gccaggcgcc ttcggcaagg
     1081 tgatgcggtg ccagctgaca tcggtgtcgg ccagcaccgc gatcacggtc gtcgccttcg
     1141 ggaacagcga cgccgcgcag cggccctgcg cctcgagggc cagcccgtcg gggctggtca
     1201 cataggcgta ctcggtgccc aggccgctcg cttcgggctc ggcgctgccc gcgctgctgc
     1261 gcaggcgctg acgcggcgga atctggacaa cgagggtcga catggacggc gggcttccgg
     1321 gctacgcgcg cattgtaggc atgcacggtt tacaaatcga agcgaatcaa tgacttacgg
     1381 catgttgttg tagcgcgctg gcagcagcgg gcggcgcgtc agctgccggg ttggtcgagg
     1441 ccggcgatgc gctcgcgctg cagcacattg acctgcccgc gcgccaggcg ctgcaccagc
     1501 gagcgctcga ccaatacgcg gtccatgagt cgtaagcggc cgcgcacctc gaagaagtcg
     1561 gaatgcacac cgatgttcgg cggtgccgat gcggcggttg cagacggcga cgcgctctgg
     1621 gccttgaagg ccggcacgtc cttgaatggc gtgcgctggc cgatctgtac caaacgctcg
     1681 gcgctcgcct gatcggtgtt gatcaccgcg ccaagcacct ctttcgatgc cgtgttgacg
     1741 ttcagcggtg tggggtccgg cagcagcacg acatagggct gcagcgcctg gatcgcggcc
     1801 ggatcgatgc cgagccaact cagctgtgcc gtcgtcttgg gcggcagcgg cgcgcccgaa
     1861 gacgccgtca tcgcggcgag caggccggtc gcaatgcgct gggccacgcc ggtctcgacg
     1921 ccgacgcgct cgcacaggcg ctccagcgtc tgcagctcgg gtcggttgat ctgcgccggt
     1981 gcggcgctgg tggccaaggt caccagattc gtcaggttgt agcgcgcctg agcgtcgctg
     2041 atgctgccgg acagaaacgc ctcggggccg tcgtcggcgt tcgccttgtc gaccgcgaga
     2101 aaggtcgaca gccgtgactc ggccagcggc gtggcccacg gttcggtgag cgcagtcggg
     2161 cggccgctct tctggtcttc gcgcaggatc agtttcgcca gttcgagcgc gcccgaaaga
     2221 atccacgccg cctgcgcgcg cgcgcgttcg gccgattcga cctgcaccgc gcgccactgc
     2281 tgccagacca tcgcggtggc cagcgtcgcg accagcgtga cgatgatcat cgcggtcagc
     2341 agagcggcac cgcgctggcg ggacggccgg tgcctggtca gacggtgggc aggcatggtg
     2401 tgtcaggggc cgcgcaaggt gtcgcgcgtc aggtcgccgt tgatgccgct gcctggcacg
     2461 aagctcagca ccacgcgcac gccgtccggc aggaccgtgc gcgttgtcaa tgggccggga
     2521 actgcgctgg ccccggccgc cgccgcaacg acgacgttgc cggtggactg gcagttcgcc
     2581 cagccgttgg tctggaagaa gtagacctgc cagccggcca cgccggtcag cgccttcagc
     2641 gaacccggct cgccgccctg caactgcagg ctgcgcagcc agctgtcctg cagcgcgctg
     2701 gtggtggtca ccgacgggcc ggcccagcgc acccacgcgc cgccgctgtc gtccggccgg
     2761 tacgaccagg cgacgacctg cagcccgtcg ggcgtgcggc gcaccatgcg caccgacgcg
     2821 ccgtcgcact tgagtgattc gggcacggcg ccgctgtcct ggatcgatgc caggtcctgg
     2881 tcccattgcg cgatcacggt gttcaggcgc agcgtctgtt cgagccgcgc ctggctcgcg
     2941 tcgcgggtgc gcacgatgcc gtccacgccc tgccaggcca tcaccgccat gatcgccatc
     3001 acgaccatcg cgaccagcac ctcgaccagc gtgaagccgc cctgtcgagc gaagcggttc
     3061 attttcgcag caccgtcgtc aggttcagga tcggctgccc gttttcgtcg gcgatggccg
     3121 cgtcgacgtt gacgaacagc gggttcggcg tcggccgcac cgacagccgg cccgcgtagg
     3181 cctggccgag ttcgacgcag ctgaactccg aatcgccgat gggcggcgtc agcttgctca
     3241 gccgcagccc ggtgagctgg ttgtcggcgc accactgcgc ggcggtcgcg tcggcaagac
     3301 gctgggtgtt gccggtcagc gcgccggcgg ccttgatgcc cgcgccgagc gtgatcgcga
     3361 cgatcgccag cgcgaccatc acctcgatca gcgtgaagcc cggcgcgcga cgcgtgctca
     3421 tcaagatcgc ggcaccgccg ccggttcgcc gtcgatcacc gtgaacgggc cgaggccgtc
     3481 ggtggcgagt gtcaggcttt gcgactcgag gtgcagcacg acgcgctgcg cgccgatcag
     3541 cggttcgggg ccgagcacga cggcgcgtgc gcccagcact tcggcagtga cccctgggac
     3601 gagccactgc gtgggcaact cggacgacgg cggcaggccc acgaagcgaa agccctcggc
     3661 gccttcctgc gcagcgcgcg gctcccagct ggccgcgagg ccagcggcgc gcgcctcgct
     3721 gcgcgccgac tcgagcagcg cgaccagccg cgcaccttcg tgctcgagcc gggtcgatgc
     3781 cgggtcgcgc agtgccaggc tggccacggc gctggcgatc gcgatcagcg cgaccacgac
     3841 catcagttcg atcagcgtga agccggctcg acgggcgttc acgtcgttca cctcattgcc
     3901 aagagccgag atcggcgttc ttgccttcgc cgccgacgac gccgtcggcg ccgaagctgt
     3961 agacatcgat ctcgcccttc aggcccgggt tcgcgtactg gtacggatgg ccccagggat
     4021 cgttcggcag cttgtcgagg ta
//
LOCUS       DATMJB010000454        10494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_263634, whole genome shotgun sequence.
ACCESSION   DATMJB010000454 DATMJB010000000
VERSION     DATMJB010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263634"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /gene="lptC"
                     /locus_tag="VNU48_09720"
     CDS             <1..564
                     /gene="lptC"
                     /locus_tag="VNU48_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HWI11595.1"
                     /translation="LPLLLMAVLALGTWWLVKNTPIFDGDRTAAPLRHEPDYTMTQFL
                     VQRFGANGAMRVQIEGDLMRHYPDTDTFEIDNPRIRALGDDGRVTVATARRALSNRDG
                     SEVQLSGGAHVVRAATATEAAIEFRGEFLHYLQYSERVRSHLPVVVTQGASEIHADAM
                     AYDHLAGVLDLKGHVRAVLSPPSGAKP"
     gene            561..1337
                     /locus_tag="VNU48_09725"
     CDS             561..1337
                     /locus_tag="VNU48_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI11596.1"
                     /translation="MKAPLVFITGASSGIGQALAARFHRAGYRLALVARRAAEVQAWA
                     QAQGFDAASFAVYAADVRDVGAIVAAGHACSAAQGVPDVVIANAGISIGMDTAEFDDL
                     DVMRATFETNNLGLAASFHPFVAPMRARRSGILVGMASVAGIRGLPGHGAYCASKAAV
                     IGYCESLRGELRASGVKVVTIVPGYVDTPLTSGNPYAMPFLMPADAFADRAFGAITRG
                     ASYRVIPWQMGVVAKLMRLLPNALFDRLVSGRGRKPRRKA"
     gene            complement(1431..1907)
                     /locus_tag="VNU48_09730"
     CDS             complement(1431..1907)
                     /locus_tag="VNU48_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HWI11597.1"
                     /translation="MGNKLYVGNLSYNIRDDDLQQAFAQYGSVSSAKVMMDRDTGRSK
                     GFGFVEMGSDAEAQAAINGMNGQALDGRAIVVNEARPREERPGGFGGGGGRSGGGGFG
                     GGGGGRSGGGGGFGSGGGGGGGYGGGGGGRSGGGGGYGGGGGSRSGGGGGGSRGGY"
     gene            2222..3004
                     /locus_tag="VNU48_09735"
     CDS             2222..3004
                     /locus_tag="VNU48_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HWI11598.1"
                     /translation="MALRIASLVPSLTELVVALGLRQALVARTGFCIHPAPALKDVPK
                     VGGTKDVNLAKLQRLAPTHVLVNVDENRLETVEAIRAWPSPPQIVVTHPNGPDDNLAL
                     VAQMVTHFGADAEVRARAAALTQALQDELALTQARGRPAQRVLYLIWRDPWMAVARDT
                     YIARMLDRIAWHTWPDVRGGAHGAARYPVVHGDEPWLADVQQVLLSSEPYHFTAAHLD
                     EARALCPNARARLVDGEMLSWYGPRALPGLRYLREIAADTRG"
     gene            3021..3659
                     /locus_tag="VNU48_09740"
     CDS             3021..3659
                     /locus_tag="VNU48_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HWI11599.1"
                     /translation="MEIYKPLIALLAIVNPIGVIPFFIHFTDGFTRAQRQRTARVAAL
                     AAFVVIAVSALAGLKIIEFFGISLASFQVGGGLLLMMSALQMLNAQPAEARRDDLHEG
                     GDKADTGASIAIVPLTIPLLTGPATISTMIIYADKTRHWWELGVLVTYGLIIGTVVWV
                     TFSLSEQVAKAIGKTGTNVMTRLMGLLLAALAVEVMSDGLVKLFPVLAGGAH"
     gene            3664..5337
                     /locus_tag="VNU48_09745"
     CDS             3664..5337
                     /locus_tag="VNU48_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWI11600.1"
                     /translation="MSDAVYDYIVCGGGTAGCLLANRLSADPNKHVLLVEAGGNDDYL
                     WVHIPVGYLYCIGNPRTDWLYSTDADPGLNGRSLRYPRGKVMGGCSSINGMIYMRGQA
                     RDYDGWDAGDGFGANPGWSWAECLPHFLKHEDFYKGADAFHAAPGFDATGARAGGEWR
                     VEKQRLRWDVLDAFAQAAQQAGIPATEDFNRGDNEGVGYFHVNQKAGIRWNATKAFLR
                     PAQRTRHNLQVWTGAQIGRVLTETRDGVPGAVGIEVLPVEGGAPVQARVAAGGEVILA
                     TGAIGTPQILQLSGIGAPELLTRLGIVVTHALPGVGANLQDHLQIRAVYGVSGVKTLN
                     TMTASWWGKALIGLEYALKRSGPMSMAPSQLGAFTRSDAGQAHPNLEYHVQPLSLDAF
                     GEPLHGYNAFTASVCNLNPTSRGAVQVTSSEPRDAPSIAPRYLSTDADRLIAANSLRL
                     TRRIVAQPALAKYRPQEVKPGAQFETDGELWKLAGDIGTTIFHPVGTAKMGPAADPMA
                     VVDARLRVHGVRGLRVVDASVMPAITSGNTNAPTLMIAERAAAWMLGGE"
     gene            5334..7622
                     /locus_tag="VNU48_09750"
     CDS             5334..7622
                     /locus_tag="VNU48_09750"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HWI11601.1"
                     /translation="MIELLVLNAAVNLAVGLALLGVWRSERSQAFARSLGWSFVVQAL
                     AAPALLAWQRGAGAWSSVGFVVLVAAVAAGLALWVAGAAELAGRSPPRRQVVWGVLGL
                     MIVGGTVLVYDPRLAGTLSATLTTLGAVVALSWLWRVGGHERIAGIAMLLLGLTQFAG
                     VAANGVGLAWQAAMATGLRVVLGLALLHAAVGRRSSEASRLRDQFRQLVERSHQGVAV
                     MQGEAMKYANPALLRIYGLPVDGGAQIVTGQQWRDATMPENERAIARERHRRIVSGEL
                     AQDHWVGERRAFDGRALHLRFSAWKVDWDGAPAEQIVVTDETAHVDATLTLLHQATHD
                     ELTGLPNRSALQQRLRVLCAAEPAQSFALLLLDIDRFKLFNEAHGSSVGDQVLRALAA
                     GLVERLGPAAQVMRFGEDEFALLASADSAARAERAARELAQGVRQLLQQPFELPQHRF
                     FLDVSIGIALYPGSATNADALLRSANAAMHEAKRTPGTSVQFAEERFERGSGATLAAE
                     QALRAGMKGDEFTLVYQPKVDARSGALVGFEALARWDRPGLGRVGPNEFIPAAERTGL
                     IGPLGNLILTLACHQIADWRETFGRMVPVAVNVSPLQLLDPGFPDLVVRTLRHFNIPP
                     PQLTLEITESAAVTHMGQAREQIAQLRAHGVEVALDDFGTGFSSLNMLRGLPLSAVKI
                     DRTLIDPMPAPDASAVVKAICDLAGALGLEVVAEGVETLAHANAALEAGCQVLQGFLY
                     ARPLEPAEAVRWMRGRPEFKES"
     gene            complement(7660..7860)
                     /locus_tag="VNU48_09755"
     CDS             complement(7660..7860)
                     /locus_tag="VNU48_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11602.1"
                     /translation="MALSTSPSSAQRPVEWMRIFATRVAQQRADLSADEAVRLAIRAF
                     QVSGHLQPEQAADRAQDSRGAS"
     gene            8286..8435
                     /locus_tag="VNU48_09760"
     CDS             8286..8435
                     /locus_tag="VNU48_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HWI11603.1"
                     /translation="MLYTIVVVLLALWVLGLVTSFTAGGLLHGLLVLAVIVLIYQLVT
                     GRRGV"
     gene            8543..10279
                     /gene="soxB"
                     /locus_tag="VNU48_09765"
     CDS             8543..10279
                     /gene="soxB"
                     /locus_tag="VNU48_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HWI11604.1"
                     /translation="MSLSRREFLQVIGAASVAGMALGRHADADARAAENALYELPRFG
                     NVSFLHMTDCHAQLRPMYFREPSVNIGVGTMQGRWPHLVGERLLKAAGVARDARTAHA
                     LSSLDFEGAARRYGKVGGFAHLATLVKRLKASRPGALLLDGGDTWQGSATALWTAGQD
                     MIDAAKLLQVDVMTGHWEFTYGMDRVKEVIEKDFKGRIDFVAQNVKTADFGDPVFAPH
                     TLREINGVRCAVIGQAFPYTPIANPRYFVADWSFGIQDAQLQTIVDEVRAKGAQVVVL
                     LSHNGMDVDLKLASKLRGIDAIFGGHTHDAVPFAVPVANGGGKTLVTNAGCNGKFLAV
                     MDFEVRDGRVADFRYRLLPVFANVLAADAEMDALIAKSRAPFERKLGEELAVSDTLLY
                     RRGNFNGSWDQLICDALLQVQGAQIAFSPGFRWGTTLLPGETITREAMMDQLAITYPH
                     TTLTEMSGETIKTILEDVADNLFNPDPYYQQGGDMVRVGGLAYRCEPGAAMGRRISGM
                     RLDGQPIEAGKTYKVAGWAPVAEAARTAGSPPIWDVVETWLRAQGGHVKAAPLNTPAL
                     VGVNGNPGLGAM"
BASE COUNT         1536 a         3727 c         3742 g         1489 t
ORIGIN      
        1 ctgccgctgc tgctgatggc ggtgcttgcg ctgggcacct ggtggctggt gaagaacacg
       61 ccgatcttcg acggcgaccg cacggcggcg ccgctgcgcc atgagcccga ctacacgatg
      121 acgcagttcc tggtgcagcg cttcggagcc aacggcgcga tgcgcgtgca gatcgagggc
      181 gacctgatgc gccactaccc ggacaccgac acgttcgaga tcgacaaccc gcgcatccgt
      241 gcgctcggcg acgacggccg cgtcaccgtc gcgacggcgc gccgggcctt gtcgaaccgc
      301 gacggcagcg aggtgcagct cagcggcggc gcccacgtgg tgcgcgccgc caccgcgacc
      361 gaagcggcga tcgagtttcg cggcgagttc ctgcactacc tgcagtacag cgagcgcgtg
      421 cgttcgcacc tgccggtcgt cgtgacccag ggcgcctccg agatccacgc cgacgcgatg
      481 gcctacgacc acctcgctgg cgtgctcgac ctcaagggcc acgtgcgcgc ggtgctgtcg
      541 ccgccgagcg gagccaagcc atgaaggcac cgcttgtgtt catcaccggc gcgtccagcg
      601 gcatcggcca ggcgctggcc gcgcgcttcc accgcgccgg ctaccggctc gcgctggtgg
      661 cgcgccgcgc cgccgaggtg caggcctggg cgcaggcgca gggcttcgat gcggcgagtt
      721 tcgcggtcta cgcggccgat gtgcgcgacg tcggcgcgat cgtcgcggcc gggcatgcgt
      781 gcagcgcggc gcagggcgtg cccgatgtcg tgatcgccaa cgccggcatc agcatcggca
      841 tggacaccgc cgagttcgac gacctcgacg tgatgcgcgc gaccttcgag accaacaatc
      901 tggggctggc ggcgagcttc catccattcg ttgcgccgat gcgtgcgcgc cgcagcggca
      961 tcctcgtggg catggccagc gtcgccggca tccgcggcct gccgggccac ggcgcctact
     1021 gcgccagcaa ggcggcggtg atcggttatt gcgaaagcct gcgcggcgag ctgcgcgcca
     1081 gcggcgtgaa ggtcgtgacg atcgtgcccg gctatgtcga cacgccgctg accagcggca
     1141 acccatacgc catgccgttc ctgatgccgg ccgacgcctt cgccgatcgg gccttcggcg
     1201 cgatcacgcg cggcgccagc taccgcgtga tcccgtggca gatgggggtc gtcgcgaagc
     1261 tgatgcggct gctgccgaac gcgctgttcg accggttggt cagcggccgt ggccgcaagc
     1321 cgcgtcggaa ggcttagccg cgcgcaggcc ttgccaacga aaaagcggcg ggcttgcgcg
     1381 ccgccgcttg atgcaccggc cggccgaagc cggttcgatg cgaatcggga tcagtagccg
     1441 ccgcggctgc caccgccgcc accgccggag cggctgccgc caccaccgcc gtagccgcca
     1501 ccaccgccgg agcggccacc accgccgcca ccgtagccac caccaccacc gccgccgctg
     1561 ccgaagccac cgccgccacc cgagcgaccg ccgccaccgc caccgaagcc gccgccgccg
     1621 gagcggccac cgccaccgcc gaagccgccc gggcgctctt cgcgcgggcg ggcctcgttg
     1681 acgacgatcg cgcgaccgtc gagggcctgg ccgttcatgc cgttgatggc cgcttgcgct
     1741 tctgcgtccg aacccatttc cacgaagccg aagcccttgg aacggccggt gtcacgatcc
     1801 atcatgacct tggcggaaga gacgctgccg tactgcgcga acgcctgctg cagatcgtca
     1861 tcgcggatgt tgtacgaaag attaccgacg tagagtttgt tgcccatggg agagcccctt
     1921 tccatttcct gaagaccatg tggagcttct acccatcgtg aaccattcaa gcgaaggcgc
     1981 tgcttccata cacagatttg tgaatataag tgaattgaac gagactgcgc acgcggcgct
     2041 cgaagaaagt gttgttttcg ggcaagcgcc tagcaacccg cgttgagccg ccgcgccgtc
     2101 ttcgcaccga tatcatccgc gctcgccctt ctcccccacc gaaccgagcc gtcagacgat
     2161 gcgcgagcgt gaccccctga gcgccccgag cctggtgggg ctggtgcagc cctgacgagg
     2221 catggcgctg cgcattgcca gcctcgtgcc gagcctgacc gagctggtcg tcgcgctcgg
     2281 cctgcgccag gcgctggtcg cgcgcaccgg cttctgcatc cacccggcgc ccgcgctgaa
     2341 agacgtgccg aaagtcggcg gcaccaagga cgtgaacctc gccaagctgc agcgcctggc
     2401 gccgacccac gtgctcgtca acgtcgacga gaaccggctc gagaccgtcg aggcgatccg
     2461 cgcctggccg agcccgccgc agatcgtcgt cacgcacccg aacggccccg acgacaacct
     2521 cgcgctggtg gcgcagatgg tcacgcactt cggtgccgac gccgaggtgc gcgcgcgcgc
     2581 cgccgcgctg acgcaggcgc tgcaagacga gctcgcgctg acgcaagcgc gcggccgccc
     2641 cgcgcagcgc gtgctctatc tgatctggcg cgatccgtgg atggcggtgg cgcgcgacac
     2701 ctacatcgcg cgcatgctcg atcgcatcgc gtggcacacc tggcccgacg tgcgcggcgg
     2761 cgcgcacggc gccgcgcgct acccggtggt gcatggcgac gagccctggc tcgccgacgt
     2821 gcagcaggtg ctgctcagct ccgagcccta ccacttcacg gccgcgcacc tggacgaagc
     2881 gcgcgcgctg tgcccgaacg cgcgcgcgcg gctcgtcgac ggcgagatgc tcagctggta
     2941 tggcccgcgc gcgctgccgg gcctgcgcta cctgcgcgaa atcgccgcgg acacgcgtgg
     3001 gtgacacgcg acaatcccgc atggagattt acaagcccct gatcgcgctg ctggcgatcg
     3061 tcaacccgat cggggtgatc ccgttcttca tccatttcac cgacggcttc acgcgtgcgc
     3121 agcgtcagcg caccgcgcgc gttgcggccc tcgcggcctt cgtcgtgatc gcggtcagcg
     3181 cgctggcggg gctgaagatc atcgagttct tcggcatctc gctggcgtcg ttccaggtcg
     3241 gcggcgggtt gctgctgatg atgtcggcgc tgcagatgct gaacgcccaa ccggccgagg
     3301 cgcgccgcga cgatctgcac gagggcggcg acaaggccga caccggcgcc agcatcgcga
     3361 tcgtgccgct gacgatcccg ctgctgaccg ggccggcgac gatctcgacg atgatcatct
     3421 acgccgacaa gaccaggcac tggtgggagc tcggcgtgct cgtcacctac gggctgatca
     3481 tcggcacggt ggtgtgggtc acgttcagcc tgtcggagca ggtcgcgaag gcgatcggca
     3541 agaccggcac caacgtgatg acgcggctga tgggcctgct gctcgcggcg ctggcggtcg
     3601 aggtgatgtc ggacgggctg gtcaagctct tcccagtgct cgcgggcggt gcgcactgac
     3661 gccatgagcg acgcggtcta cgactacatc gtctgcggcg gcggcaccgc cgggtgcctg
     3721 ctggcgaacc gcctgagcgc cgacccgaac aagcacgtgc tgctggtcga ggccggcggc
     3781 aacgacgact acctgtgggt gcacatcccg gtcggctacc tgtactgcat cggcaacccg
     3841 cgcaccgact ggctctattc gaccgatgcc gacccgggtc tgaacggtcg gagcctgcgc
     3901 tacccgcgcg gcaaggtgat gggcggctgc agcagcatca acggcatgat ctacatgcgc
     3961 ggccaggcgc gcgactacga cggctgggac gccggcgacg gcttcggcgc caaccccggc
     4021 tggagctggg ccgagtgcct gccgcacttc ctgaagcacg aggacttcta caagggcgcc
     4081 gacgccttcc acgccgcgcc cggcttcgat gcgaccggcg cacgcgccgg cggcgaatgg
     4141 cgcgtcgaga agcagcgcct gcgctgggac gtgctggacg cattcgcgca ggccgcgcag
     4201 caggccggca tcccggcgac cgaagatttc aaccgcggcg acaacgaggg cgtcggctac
     4261 ttccacgtca accagaaggc cggcatccgc tggaacgcga ccaaggcctt cctgcgcccg
     4321 gcccagcgca cgcgccacaa cctgcaggtc tggaccggcg cgcagatcgg ccgcgtgctg
     4381 accgagacgc gcgacggcgt gcccggcgcg gtcggcatcg aggtgctgcc ggtcgaaggc
     4441 ggcgcgccgg tgcaggcgcg cgtggccgcc ggcggcgagg tgatcctggc gaccggcgcg
     4501 atcggcacgc cgcagatcct gcagctgtcg ggcatcggcg cgcccgagtt gttgacgcgg
     4561 ctcggcatcg tggtcacgca cgcgctgccc ggcgtcggcg cgaacctgca ggaccatctg
     4621 cagatccgcg ccgtctacgg cgtcagcggc gtgaagacgc tgaacacgat gaccgcgtcg
     4681 tggtggggca aggcgctgat cggcctcgag tacgcgctca agcgcagcgg cccgatgagc
     4741 atggcgccgt cgcagctcgg cgcgttcacg cgcagcgacg cgggccaggc gcatccgaac
     4801 ctcgagtatc acgtgcagcc gctgtcgctc gacgcgttcg gcgagccgct gcacggctac
     4861 aacgcgttca ccgccagcgt gtgcaacctg aacccgacca gccgcggcgc ggtgcaggtc
     4921 acgtcgagcg agccgcgcga tgcgccgagc atcgcgccgc gctacctgag taccgacgcc
     4981 gaccgcctga tcgccgcgaa cagcctgcgc ctgacgcggc gcatcgtcgc gcagccggcg
     5041 ctggcgaagt accggccgca ggaggtcaaa cccggcgcgc agttcgagac cgacggcgag
     5101 ctctggaagc tcgccggcga catcggcacg accatcttcc acccggtcgg caccgcgaag
     5161 atgggccctg ccgccgaccc gatggcggtg gtcgacgcgc ggctgcgcgt gcacggcgtg
     5221 cgcggcctgc gcgtcgtcga tgcgagcgtg atgccggcca tcaccagcgg caacacgaac
     5281 gcgccgacgc tgatgatcgc ggaacgcgcc gcggcctgga tgctgggcgg cgaatgatcg
     5341 agctgctggt cctgaatgcg gcggtcaacc tggcggtcgg cctggcgctg ctgggcgtgt
     5401 ggcgcagcga acgcagccag gccttcgcgc gatcgcttgg gtggagcttc gttgtgcagg
     5461 cgcttgccgc gcccgccctg ctggcgtggc agcgcggcgc gggtgcgtgg tcgtcggtcg
     5521 ggttcgtcgt gctcgtcgcc gccgtcgcgg ccggtctggc gctgtgggtc gcgggcgccg
     5581 cggaactcgc cggccgctcg ccgccgcgtc gccaggtcgt ctggggcgtc ctcggcctga
     5641 tgatcgtcgg cggcacggtg ctcgtctacg acccgcgcct ggccggcacc ctgagcgcca
     5701 cactgaccac gctcggcgct gtcgtggcgc tgagctggct gtggcgcgtc ggcggccacg
     5761 aacgcatcgc cggcatcgcc atgctgctgc tcgggctgac gcagttcgcc ggcgtcgccg
     5821 ccaacggggt cgggctggcg tggcaggcgg cgatggcgac ggggttgcgg gtcgtcctgg
     5881 ggctggcgct gctgcacgcc gcggtgggtc ggcgcagcag cgaggcgagc cgcctgcgcg
     5941 accagttccg gcagctggtc gagcgctcgc accagggtgt ggccgtgatg cagggcgagg
     6001 ccatgaagta cgcgaacccg gcgctgttgc gcatctacgg gctgccggtc gacggtggcg
     6061 cgcagatcgt caccggccag caatggcgcg atgcgacgat gcccgagaac gagcgcgcga
     6121 tcgcgcgcga gcgccaccgc cgcatcgtca gcggcgagct cgcgcaggac cactgggtcg
     6181 gcgaacgccg cgccttcgac ggccgcgcgc tgcacctgcg cttcagcgcc tggaaggtcg
     6241 actgggacgg cgcccccgcc gagcagatcg tcgtcaccga cgagaccgcg catgtcgacg
     6301 cgaccctgac cctgctgcac caggccacgc acgacgagct caccggcctg cccaaccgca
     6361 gcgccttgca gcagcgcctg cgcgtgctgt gcgcggccga gccggcgcag tcgttcgcgc
     6421 tgctgctgct cgacatcgac cgcttcaagc tcttcaacga agcgcacggc tcgtcggtcg
     6481 gcgaccaggt gctgcgcgcg ctcgctgccg gcctggtcga acggctcggc ccggcggccc
     6541 aggtgatgcg cttcggcgag gacgagttcg cgctgctcgc gtcggccgac agcgccgcgc
     6601 gcgccgagcg cgccgcgcgc gaactggcgc agggcgtgcg ccagctgctg cagcagccgt
     6661 tcgagctgcc gcagcaccgc ttcttcctcg atgtgtcgat cggcatcgcg ctgtaccccg
     6721 gcagcgcgac gaacgccgac gcgctcttgc gctcggccaa cgcggcgatg cacgaggcca
     6781 agcgcacgcc cggcacctcg gtgcagttcg ccgaagagcg cttcgagcgc gggtcgggtg
     6841 cgacgctggc ggccgagcag gcgctgcgcg ccggcatgaa gggcgacgag ttcacgctcg
     6901 tctaccagcc caaggtcgat gcccgcagcg gcgcgctggt cggcttcgag gcgctcgcgc
     6961 gctgggaccg cccgggcctg ggccgggtcg ggccgaacga gttcattccc gcggccgagc
     7021 gcaccgggct gatcgggccg ctcggcaacc tgatcctgac gctggcctgc caccagatcg
     7081 ccgactggcg cgagaccttc ggccgcatgg tgccggtggc ggtcaacgtg tcgccgctgc
     7141 agctgctcga ccccgggttt cccgatctcg tcgtgcgcac gctgcgccac ttcaacatcc
     7201 cgccgccgca gctgacgctg gagatcaccg agagcgccgc ggtgacgcac atggggcagg
     7261 cgcgcgagca gattgcacag ctgcgcgcgc acggcgtcga ggtcgcgctc gacgacttcg
     7321 gcaccggctt ctcgtcgctg aacatgctgc gcgggctgcc gctgtcggcc gtcaagatcg
     7381 accgcacgct gatcgacccg atgcctgcgc ccgatgccag cgcggtcgtc aaggcgatct
     7441 gcgacctggc cggggcgctc gggctcgaag tcgttgccga aggggtcgag acgctcgcgc
     7501 atgcgaacgc tgcgctcgag gccggctgcc aggtgctgca gggcttcctg tatgcgcggc
     7561 cgctggagcc ggccgaggcg gtgcgctgga tgcgcggccg gcccgaattc aaggagtcat
     7621 gagctcggcg gctttgcctg gttcacggcc gccgggccct cacgacgcac cccgcgaatc
     7681 ctgcgcgcga tcggcggcct gctcgggctg caggtggccc gacacctgaa atgcgcggat
     7741 cgccaggcgc acggcctcgt cggcgctgag gtccgcgcgt tgctgcgcga cacgggtcgc
     7801 gaagatgcgc atccattcga ccggccgctg cgcggaagaa ggcgatgtgg acagggccat
     7861 gtgcaggctt cggctcaggc gtgcgtgtcg tcgctcagcc gaggagccaa gtcagccgcc
     7921 gatacgtcag ccgcgacgcg tgctggggca gcaggggggc gcgatggccg gtgtcgtcga
     7981 gcgagggaca tgttcaactc cagattccgg cacgcaaacc gcgcccggtg acgaccgcgc
     8041 ccggtgacgg gatgttcggc gccgcgcgag cgcgccgcag ccggacgacg gccggcgcgc
     8101 gtgtaggcgc gctccgtcag gtcggtgcgt ggcggtgtgg cccgatcgcg cgggccaccg
     8161 gtgggacgcg gactacagcg cgtcggcggc tcggctgatt ccggcgcgtc gacctgcggc
     8221 ggaagctgca cagcaccggg gcggacgcat cgtgcagccg gcccggccat cgaaggagac
     8281 cgttcatgct ctacaccatc gtcgtggtcc tgttggcact ctgggtgctg ggcctggtca
     8341 cctcgttcac ggccggcggg ctgctgcacg gcctgctggt tctcgcggtc atcgtgctga
     8401 tctatcaact ggtgaccggc cggcgcggcg tctagcgggc gcggtgccgg caccgcgggc
     8461 acctggggca cactgcgggc ggcgcgggcg ccgccgccag cgccagttcc gctgctttcc
     8521 ccatgacgcc gacgcccgcc cgatgagcct gtccagacgc gagttcctgc aggtcatcgg
     8581 cgcggcgtcg gtggccggca tggcgctggg ccggcatgcc gatgccgacg cgcgcgcggc
     8641 cgagaacgcg ctgtacgagt tgccgcgctt cggcaacgtc tcgttcctgc acatgacgga
     8701 ttgccatgcg cagttgcgcc cgatgtactt ccgcgagccg agcgtcaaca tcggcgtcgg
     8761 cacgatgcag ggccgatggc cgcatctcgt cggcgagcga ttgctgaagg ccgccggcgt
     8821 tgcgcgcgac gcgcgcacgg cgcacgcgct gagctcgctc gacttcgaag gcgccgcacg
     8881 ccgctacggc aaggtcggcg gcttcgcgca cctggcgacg ctggtcaagc gcctgaaggc
     8941 gagccgcccc ggcgcgctgc tgctcgacgg cggcgacacc tggcagggct cggccaccgc
     9001 gctgtggacc gcgggccagg acatgatcga cgccgccaag ctgctgcagg tcgacgtgat
     9061 gaccggccac tgggaattca cctacggcat ggaccgggtg aaagaggtga tcgagaagga
     9121 cttcaagggc cgcatcgact tcgtcgcgca gaacgtcaag accgccgact tcggcgaccc
     9181 ggtgttcgcg ccccatacgc tgcgcgagat caatggcgtg cgctgcgccg tcatcggcca
     9241 ggcctttccg tacacgccga tcgcgaaccc gcgctacttc gtcgccgact ggagcttcgg
     9301 catccaggac gcgcagctgc agacgatcgt cgacgaggtg cgcgccaagg gcgcacaggt
     9361 cgtcgtgctg ctgtcgcaca acggcatgga tgtcgacctg aagctggcat cgaagctgcg
     9421 cggcatcgac gcgatcttcg gcggccatac gcatgacgcg gtgccgttcg cggtgccggt
     9481 cgcgaacggt ggcggcaaga cgctggtcac gaacgccggc tgcaacggca agttcctggc
     9541 ggtgatggac ttcgaggtcc gcgacggccg tgtcgcggac ttccgctacc gcctgctgcc
     9601 ggtgttcgcc aacgtgctcg cggccgatgc cgagatggac gcgctgatcg cgaagtcgcg
     9661 cgcgccgttc gagcgcaagc tgggagaaga gctcgccgtc agcgacacgc tgctgtaccg
     9721 gcgcggcaac ttcaacggct cgtgggacca gctgatctgc gacgcgctgt tgcaggtgca
     9781 gggcgcgcag atcgcgttct cgcccggctt ccgctggggc acgacgctgc tgcccggcga
     9841 gaccatcacg cgcgaggcga tgatggacca gctcgcgatc acctacccgc acacgacgct
     9901 caccgagatg agcggcgaga ccatcaagac gatcctcgag gacgtggccg acaacctgtt
     9961 caaccccgac ccgtactacc agcagggcgg cgacatggtg cgcgtcggcg gcctcgcgta
    10021 ccgctgcgag ccgggcgcgg cgatgggccg gcgcatcagc ggcatgcggc tggacggcca
    10081 gcccatcgag gccggcaaga cctacaaggt cgcgggctgg gcgccggtgg cggaagcggc
    10141 gcgcacggcc ggcagcccgc cgatctggga cgtggtcgaa acctggctca gggcgcaggg
    10201 cggccacgtc aaggccgcgc cgctgaacac gccggcgctg gtcggtgtga atggcaaccc
    10261 cggtctcggg gcgatgtgac ggcgacggcc tggcggagca gaatcgactc gcgacgcagc
    10321 gcgccggcgg ccccgtgatc ggcgtcggct gacagcacgt tgcggcggtg tccgagccgc
    10381 gcgcggcgcg cacacggcga agatcgcgcg atccaccgga gcgacccacc atggccacgc
    10441 aacacccgca caagccgcac cccagccacg atgtggcggc ggacgatccc gaac
//
LOCUS       DATMJB010000455         2143 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_420945, whole genome shotgun sequence.
ACCESSION   DATMJB010000455 DATMJB010000000
VERSION     DATMJB010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420945"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VNU48_09770"
     CDS             complement(<1..636)
                     /locus_tag="VNU48_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11605.1"
                     /translation="MTDIDARPTTPIRWGRWAAGVVAAVIVGLGACEAMGWPFLAGPM
                     QRWLGETLARRVSLSGNPATDPKVEIHLLGGIRIAADYIEIGAPLWSDAPHMLLARQA
                     HMTLGYTDLWRAGRGAPLRIRVLRAAQLDAHIERRADGRASWQFGRNTDTPDTTDRPT
                     RIPLFGRLQVDAGALTYRDALMAIDLDARYALVEGTNVSAADLTPGPAKGLQ"
     gene            complement(740..1732)
                     /locus_tag="VNU48_09775"
     CDS             complement(740..1732)
                     /locus_tag="VNU48_09775"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HWI11606.1"
                     /translation="MNKPAVLVCRAVFPDIVDRLREHFEVETNEADVVWSPAELIQHL
                     QGKAGVFITGTQPIDTALLAACPQLKAVCSMAVGYNNVDLPACTARGVLVSNAPDVLT
                     ETTADFGFALMMAAARRIAESEHFLRSGAWTKWSVDAFAGTDVHGATLGVLGMGRIGQ
                     AIARRGALGFGMRVIYHNRSRLDASIEAELGARWVEKDALLRDSDHLVIVVPYSAASH
                     HLVGAAELALMKPTATLTNIARGGIVDDAALAQALRQHRIAAAGLDVFEGEPAVHPDL
                     LTLPNVVLTPHIASASLPTRRAMANLAVDNLIAALGFGPAKGRPPSALNPQVLG"
     gene            complement(1729..>2143)
                     /locus_tag="VNU48_09780"
     CDS             complement(1729..>2143)
                     /locus_tag="VNU48_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HWI11607.1"
                     /translation="GSVFLLSLVLCAAMMPVEHLPPASWPVALGLGFVSAVFDNIPLT
                     ALALRQGGYDWGFLAYAVGFGGSMIWFGSSAGVALAGMFPQARSVGAWISGGWHVAVG
                     YLLGFAVLLAVLGWHPDPAHRTAAGAAPVHSESKP"
BASE COUNT          309 a          753 c          788 g          293 t
ORIGIN      
        1 ctgcaggccc ttcgcagggc cgggtgtcag gtcggcggcc gagacattgg tgccctcgac
       61 cagcgcatag cgcgcgtcga gatcgatcgc catcaacgca tcgcggtagg tcagcgcgcc
      121 cgcgtcgacc tggagccggc cgaacagcgg gatgcgtgtc ggccggtcgg tcgtgtcggg
      181 cgtgtcggtg ttgcgcccga actgccacga ggcgcggccg tcggcgcggc gctcgatgtg
      241 cgcgtcgagt tgcgcggcgc gcagcacgcg gatgcgcaag ggcgcaccgc ggccggcgcg
      301 ccacaggtcc gtgtagccca gcgtcatgtg cgcctggcgc gcgagcagca tgtgcggcgc
      361 gtcgctccac agcggcgcgc cgatctcgat gtagtcggcg gcgatgcgga tgccgccgag
      421 cagatggatc tcgaccttcg ggtccgtggc cgggttgccg ctcaggctca cgcgccgggc
      481 cagtgtctcg ccgagccagc gctgcatcgg gccggcgagg aacggccagc ccatcgcctc
      541 gcacgcgccc agcccgacga tcacggccgc gaccacgccg gcggcccagc ggccccagcg
      601 tatcggcgtg gtggggcgag catcgatgtc agtcatcgaa ccatcgtcgc acgcgggccg
      661 cgcacgcccc atcgggggtg ggcggcttcg gttgtagttg cgttcctaca gtcgtcgccc
      721 gcgcctcgga tgttccggct cagcccagca cctgcgggtt cagcgcgctc ggcggtcgac
      781 ccttcgcggg gccgaagccg agcgcggcga tcaggttgtc gaccgcgagg ttcgccatcg
      841 cgcgccgcgt cggcaggctc gcgctggcga tgtgcggcgt cagcacgacg ttcggcagcg
      901 tcagcaggtc ggggtggacg gccggctcgc cctcgaacac atcgagcccg gccgcggcga
      961 tgcggtgttg acgcagcgcc tgcgcgagcg ccgcgtcgtc gacgatgccg ccgcgcgcga
     1021 tgttggtcag cgtcgcggtc ggcttcatca gcgcgagctc ggccgcgccg acgaggtgat
     1081 gcgacgcggc gctgtagggc acgacgatca cgaggtggtc gctgtcgcgc agcagcgcgt
     1141 ccttctcgac ccagcgcgcg ccgagttcgg cctcgatcga cgcatcgagc cgcgagcggt
     1201 tgtggtagat cacgcgcatg ccgaagccga gcgcgccgcg ccgcgcgatg gcctggccga
     1261 tgcgccccat gccgagcacg ccgagcgtcg cgccatgcac gtcggtgccg gcgaaggcgt
     1321 cgacgctcca tttcgtccac gcgccgctgc gcaggaaatg ttcgctctcg gcaatgcgcc
     1381 gcgccgcggc catcatcagc gcgaagccga agtcggcggt ggtctcggtc agcacgtcgg
     1441 gcgcgttgct gaccagcacg ccgcgcgcgg tgcaggccgg caggtcgacg ttgttgtagc
     1501 ccaccgccat gctgcacacc gccttgagct gcgggcaggc ggccagcagc gcggtgtcga
     1561 tcggctgcgt gccggtgatg aacacgccgg ccttgccctg caggtgctgg atcagctcgg
     1621 ccggcgacca gacgacatcg gcctcgttgg tctcgacctc gaagtgttcg cgcagccgat
     1681 cgacgatgtc ggggaacacg gcgcggcaga cgaggaccgc tggcttgttc atggtttgct
     1741 ttcgctgtgc accggcgccg cgccggcggc cgtgcggtgc gcggggtcgg ggtgccatcc
     1801 cagcacggcc agcagaaccg cgaaaccgag caggtagccg accgcgacat gccagccgcc
     1861 gctgatccag gcaccgaccg agcgcgcctg cgggaacatg ccggcgagcg cgacgccggc
     1921 cgacgagccg aaccagatca tcgagccgcc gaagccgacc gcgtaggcaa gaaatcccca
     1981 gtcgtagccg ccttggcgca aggcgagcgc ggtcaagggg atgttgtcga acaccgccga
     2041 gacgaagccg aggccgagcg ccaccggcca cgaggccggc ggcaagtgct ctaccggcat
     2101 catcgccgcg cacagcacca gcgagagcag gaacacgctg ccg
//
LOCUS       DATMJB010000456         2079 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_493271, whole genome shotgun sequence.
ACCESSION   DATMJB010000456 DATMJB010000000
VERSION     DATMJB010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493271"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VNU48_09785"
     CDS             <1..603
                     /locus_tag="VNU48_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWI11608.1"
                     /translation="DPHAVEIPSQPSVPGSARRGRRLRNAFTFGALLLAGLSVPLRAL
                     PEAARPVCGATPSPESVLNMLNAMRTLGTVCHQTGDVAVAAPLRWSASLAAVAEAQSH
                     DMAALNRMGHRDSQNRNLTERLAAMGYRFSAAAENVAVGYPSLGEVVDAWLESEPHCD
                     NLMNAKVLELGLACSDAGPGGDPGEYRYWTLVLGAPPRAR"
     gene            complement(614..1741)
                     /locus_tag="VNU48_09790"
     CDS             complement(614..1741)
                     /locus_tag="VNU48_09790"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HWI11609.1"
                     /translation="MPLGPFGPSQALSLGVELELQLVNTVDFDLTSSSNDLLHLLSRK
                     PFPGTVTPEMTQSMIEIATGIQHEHAPLLAELRGMRDTLTAACDRLNIDIAGGGTHPF
                     QRWSEQRVFEKPRYQQLSQLYGYLAKQFTVFGQHVHVGCASGDDALFLLHSLNRYVPH
                     FIALSASSPFFQGVDTLFDSARLNSVFAFPLSGRAPFTLSWDDFANDYFTKMEATGVV
                     KSMKDFYWDIRPKPEFGTIELRVCDTPLTVERAAALACYLQAICRRLLERVEPMPQED
                     DYLVYTFNRFQACRFGLDGTMVDPQTRESRTIREDILATLRRVEVHGAVLRSLPALEE
                     IERCAASAGNHASHLRGIYARSRNVQSMVQESTRALRAGPG"
BASE COUNT          311 a          714 c          735 g          319 t
ORIGIN      
        1 gatccccacg ctgtggaaat accttctcag ccttccgtcc ctggctcggc acgtcgcggc
       61 cggcgcctgc gcaacgcgtt cacgttcggc gcgctgctgc tggccggcct gagcgtgccg
      121 ttgcgcgcgc tgcccgaggc cgcccggccg gtctgcggcg cgacgccgtc gcccgagagc
      181 gtgctgaaca tgctcaatgc gatgcgcacg ctcggcacgg tctgccacca gaccggcgac
      241 gtggccgtcg ccgcgccgct gcgctggagc gcgagcctcg cggcggtggc cgaggcccag
      301 tcgcacgaca tggcggcgct gaaccgcatg ggccatcgcg acagccagaa ccgcaacctg
      361 accgaacgac tggcggcgat gggctaccgc ttcagcgccg cggccgagaa cgtcgcggtc
      421 ggctacccgt cgctcggcga agtcgtcgat gcctggctcg agagcgagcc gcactgcgac
      481 aacctgatga acgcgaaggt gctcgagctc ggcctggcct gcagcgatgc cggcccaggc
      541 ggcgaccccg gcgagtaccg ctactggacg ctggtgctgg gcgcgccgcc gcgcgcgcgc
      601 tgacgacccg cgctcaaccg ggcccggcgc gcagcgcgcg cgtgctctcc tgcaccatgc
      661 tctgcacgtt gcgcgatcgc gcatagatgc cgcgcagatg cgacgcgtgg ttgccggcgc
      721 tggccgcgca tcgctcgatc tcttcgagcg cgggcaggct gcgcagcacc gcaccgtgca
      781 cctcgactcg gcgcagcgtg gcgaggatgt cttcgcgaat cgtgcggctc tcgcgcgtct
      841 gcggatcgac catcgtgccg tccaggccga agcggcaagc ctggaagcgg ttgaaggtgt
      901 agaccaggta atcgtcttcc tgcggcatcg gctcgacgcg ctcgagcagg cgccggcaga
      961 tcgcctgcag atagcacgcg agcgccgccg cgcgctcgac cgtcagcggc gtgtcgcaca
     1021 cgcgcagctc gatcgtgccg aactcgggct tggggcggat gtcccagtag aagtccttca
     1081 tcgacttgac gacgccggtg gcttccatct tcgtgaagta gtcgttcgcg aagtcgtccc
     1141 agctcagggt gaacggcgcg cggccgctga gcgggaacgc gaacaccgag ttcaggcgcg
     1201 ccgaatcgaa cagcgtgtcg acgccctgga agaacggcga cgaggcgctc agtgcgatga
     1261 aatgcggcac atagcggttc agcgagtgca gcagaaacag ggcatcgtcg cccgatgcac
     1321 agccgacgtg aacgtgctgg ccgaacaccg tgaactgctt cgccagatag ccgtagagct
     1381 gcgacagctg ctgatagcgc ggcttctcga acacgcgctg ctccgaccag cgctggaacg
     1441 ggtgcgtgcc gccgccggcg atgtcgatgt tcaggcggtc gcaggccgcg gtcagcgtgt
     1501 cgcgcatgcc gcgcaactcg gccagcagcg gcgcgtgctc gtgctggata ccggtggcga
     1561 tctcgatcat gctctgcgtc atctcgggcg tgaccgtgcc ggggaagggc ttgcgcgaca
     1621 gcaggtgcag caggtcgttg gagctcgagg tcaggtcgaa gtcgacggtg ttgacgagtt
     1681 gcagctcgag ctcaacgccg agcgacagcg cctgcgatgg gccgaaaggt ccgagcggca
     1741 tctcagtggc tccgccgcgc tgcgtgcgcc tggcgacgcg tgggacgagt cgcgggcgcg
     1801 gcggactgct ggcggccctt tgccgacgct cgaacttggt ttgcgtccgt cggcgcggca
     1861 aaggcatgcc cgggcaggct gctccgcttc actgcggcgg tcgtcgctgc gcgccgactg
     1921 ccctgcgctg ctcggtcccg tggcccgtcg ccaaactcac ttcgctcact gcgttcgctg
     1981 cgttcaaaca gacggcgaca agtcagttca cgaagcgcgc tgcgcgcgcg ggccacgagc
     2041 cctgcgctgc tcggcgccgc agaggcgctg cgcagcctg
//
LOCUS       DATMJB010000457         2316 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_739175, whole genome shotgun sequence.
ACCESSION   DATMJB010000457 DATMJB010000000
VERSION     DATMJB010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739175"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VNU48_09795"
     CDS             <1..462
                     /locus_tag="VNU48_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11610.1"
                     /translation="DFMEMAQLASEAGFPIEARKILDEGFATNVLGTGPDAVRQKRLR
                     DTTTKQAAEDAKELAQGRGPSAAAKDGTGSVNVGLDYVINGQFDKGLPMMEQGVAKGG
                     LKQPNDAKLRLGMAYFLAGQKDKAIEVLKAVQGADGTADLARLWILHAQRS"
     gene            489..1442
                     /locus_tag="VNU48_09800"
     CDS             489..1442
                     /locus_tag="VNU48_09800"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HWI11611.1"
                     /translation="MTACPSQAITKASLRWLLVAAIGWSFAGAAGAQANAKSVESFAD
                     CPECPLMVKVPAGEFLMGSPAQEKGREADEGPQHRVRLAAPLAVAINKVTRGQYAAFV
                     AATRRPDGDGCWVWDVGRGLWVKDAARNWRNPGFDQTDQHPVVCVSWDDAQAYAVWVS
                     QKAGKRLRLLTEAEREYVMRAGSASSRPWGEDIEAACGYANVADQTAAAKVQRIPTWI
                     ECSDGYAYTSPVAAFKPNAFGLFDTMGNAWEWVEDCYHDNYQGAPSDGSAWTTGACNE
                     RVFRGGSWDNYPRSTRSAFRYRGVPATRSSSVGFRIGRALP"
     gene            1456..2268
                     /locus_tag="VNU48_09805"
     CDS             1456..2268
                     /locus_tag="VNU48_09805"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI11612.1"
                     /translation="MMISSPFTSGSQDRDAQPRHNTWADVAFNCAGRRVLVTGGTSGI
                     GAAIAAAYQVAGAQVTVTGTRASAQDYDADLTDYQYLALSASDNAQIDRLASHFDALD
                     ILVNNAGAVWPGGRSEYEPDVFEEALRINLASGYRLAHACRPLLARSTAPAGGCVIGI
                     ASMASYFGIEAVPGYGAAKAGVVQLTKTLAVAWAGDRIRVNAVAAGLTETRMTAGHIA
                     DPQALAPTLARTPLRRVGKPSDIAAAVLFLTSDAAAYITGQTLPVDGGYSIA"
BASE COUNT          409 a          751 c          799 g          357 t
ORIGIN      
        1 gatttcatgg agatggctca actcgcttcg gaggccggct ttccgatcga agccaggaag
       61 attctggacg agggcttcgc caccaacgtg ctcggcacgg gcccggatgc ggtgcgacag
      121 aagcgcctgc gcgataccac gaccaagcag gcggcggaag acgcgaaaga gctcgcccaa
      181 ggccggggcc cgagtgctgc tgccaaggat ggcaccgggt ccgtcaacgt cggcctcgac
      241 tacgtgatca acggccagtt cgacaagggc ttgccgatga tggagcaagg cgtggcgaag
      301 ggcggcttga agcaacccaa cgacgccaag cttcgtctcg gcatggccta cttcctcgcc
      361 ggccagaaag acaaagcgat cgaggtgctc aaggccgtgc agggcgcgga cggcacagcg
      421 gatctcgccc gcctgtggat cctgcacgcg cagcggtctt gagtcaccca acgctacgga
      481 gccattccat gacagcctgt ccatcacaag ccatcaccaa agcgagcctg cgttggcttc
      541 tcgtcgccgc gatcgggtgg tcattcgcgg gggccgcggg tgcgcaggcg aacgcaaaga
      601 gtgtcgagtc gttcgccgat tgccccgagt gtcccctgat ggtgaaagtg cccgcaggcg
      661 agttcctgat gggctcgcca gcgcaggaaa aagggcgcga agcggatgaa ggtccgcagc
      721 atcgtgtgcg cttggcggcg ccgctggcgg tcgcgatcaa caaggtgacg cgcggtcaat
      781 atgcagcgtt cgttgccgcg acacggcgtc ccgatggcga cggttgttgg gtctgggacg
      841 tcggccgtgg cctgtgggtc aaggatgcgg cgcgcaactg gcgcaatccc ggcttcgatc
      901 agacggatca gcacccggtc gtgtgcgtga gctgggacga cgcacaagcc tacgcggtgt
      961 gggtgagcca gaaggcgggc aagcgcttgc gccttctcac cgaggccgag cgggagtacg
     1021 tgatgcgtgc cgggagtgcg tcatcgcggc cctggggcga agacatcgaa gccgcgtgtg
     1081 gctacgccaa cgtggccgac cagaccgcgg ccgccaaggt gcagcgcatt cccacctgga
     1141 tcgagtgttc ggacggatac gcctacacct cgccagtggc ggccttcaag cccaacgcat
     1201 tcggcctgtt cgacacgatg ggcaacgcct gggaatgggt cgaagactgc taccacgaca
     1261 actaccaggg cgcgccgtcg gatggcagcg cctggaccac gggagcctgc aacgagcgtg
     1321 tctttcgggg cggcagctgg gacaactacc cgcgcagtac ccgatcggca ttccgctacc
     1381 ggggtgtgcc ggcgacccga agcagcagcg tcggctttcg catcgggcga gcgctgcctt
     1441 gatcggcacc gctggatgat gatctcgagt ccgttcacgt cgggatcgca ggatcgcgat
     1501 gcgcagccga ggcacaacac gtgggccgat gtcgcgttca actgtgccgg ccgccgtgtg
     1561 ctggtcacgg gcggcaccag cggaatcggc gctgccatcg ctgcggcgta ccaggtggcg
     1621 ggcgcgcagg tgaccgtcac gggaacccgc gcatcggccc aagactacga cgccgatctc
     1681 accgactacc agtatctggc gctgagtgca tcggacaacg cgcagatcga tcgccttgca
     1741 tcgcacttcg acgcgctcga cattctggtg aacaacgcag gtgcggtgtg gcccggcggg
     1801 cgcagcgagt acgaacccga cgtgttcgaa gaggccttgc gcatcaacct ggccagcggc
     1861 tatcgactgg cgcatgcctg ccgcccgctg ctcgctcgca gcaccgcccc cgcgggcggc
     1921 tgcgtcatcg gcatcgcgtc gatggcgtcc tacttcggca tcgaggccgt gccgggatat
     1981 ggcgcggcca aggccggcgt ggtccagttg accaagacgc ttgcggtggc gtgggcgggt
     2041 gaccggattc gcgtcaacgc cgtggctgcc gggctgaccg agacgcgcat gacggcaggg
     2101 cacattgccg acccccaggc gctggcgccc accctggcgc gaacgccgct gcgacgcgtt
     2161 ggcaagccgt cggacatcgc cgcggcggtg ctgttcctga ccagtgacgc agcggcctac
     2221 atcaccgggc agaccctgcc cgtcgatggc ggctactcga tcgcctgaaa ccctcattcc
     2281 gggcacgccc aggccgaccg atgacccacg ccgctg
//
LOCUS       DATMJB010000458         2145 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_563355, whole genome shotgun sequence.
ACCESSION   DATMJB010000458 DATMJB010000000
VERSION     DATMJB010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563355"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="VNU48_09810"
     CDS             complement(<1..610)
                     /locus_tag="VNU48_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11613.1"
                     /translation="MARAHAADACVQSDSIKGLAQSIAKPAYHRLLVAEPALRRAVPT
                     LIIAFLITICLGAFVQVIDQNRQKRAAMKRDLAAVADMLAERLDRIASVRQDRVQTVD
                     RLQSLLPGLVPSWGVAAGRHVIVTGGDQRVLARVPTETALGDTAGILELVGAALPLNA
                     PVQQQGVTDITLPNGNSALAVRQLVKALPGQVIVIQEKIDSLW"
     gene            complement(800..>2145)
                     /locus_tag="VNU48_09815"
     CDS             complement(800..>2145)
                     /locus_tag="VNU48_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HWI11614.1"
                     /translation="AAISIFASAGMAAAKFTVGIAIGSLALISEALHSSVDVVATVIT
                     WLVVRVSDRPADEEHHYGHGKLESLSALGVIALLYVLAGGILVESVSRLREGAPPPTL
                     SAIPFVVLVIDIAVNFWRARALHRAARDTRSQALAADALHFASDVLGSIAVIFGLALS
                     GLGYAWGDSAAAIGVAIMISVLGLRLGRSTVETLVDRAPEGVSEKAIEAIKAVPGVID
                     VERLRVRMVGPTYFIDAIVQVPRTFPIDRVDAIKRKAQAGVSQVFDDADLTFTAVPVA
                     RDNESVRERIMVIARNSGLAVHHVTVHDLGDRLTVSIDLEVDGEMALTSAHDIAHDLE
                     SNIRHEFGEDVEVDTHIEPLEPELPLGSDAAPDRVAVIKSALTRFAAEGAVHDIHNVR
                     VRNTEAGEIVNFHCRAAPSMSVIKVHENVDEIERALRRAFPTVKRVISHAEPPYA"
BASE COUNT          396 a          674 c          707 g          368 t
ORIGIN      
        1 gccagagtga atcgatcttt tcctggatga cgattacctg gccgggcagc gccttgacca
       61 gttggcggac cgccagtgcg ctgttgccgt ttggcagggt aatgtcggtg acgccctgct
      121 gctgaaccgg agcgttgagc ggcaatgccg cgccgaccag ttcgagaatt ccggcggtgt
      181 cgcccagcgc ggtctcggtc gggacccggg ccagaacgcg ctggtcgccg ccggtaacta
      241 tgacgtgtcg cccggcggcg acgccccagg atggaacgag gccgggcagc aggctctgaa
      301 gtcgatcgac ggtctggacg cgatcctgcc gaaccgaggc gatgcggtcg aggcgctcgg
      361 ccagcatatc ggcgacagcc gcgagatcgc gtttcatcgc cgcccgcttt tgccggttct
      421 ggtcgatcac ctgcacaaac gcgccaaggc agatggtgat caggaaagca atgatcaggg
      481 taggcaccgc gcggcgcagc gcgggttcag cgaccagtag cctgtgatag gccggtttcg
      541 cgatcgattg cgccaatccc ttgatcgaat cggattggac gcacgcgtcc gccgcatgcg
      601 cgcgcgccat gccttgaccc ccagaaatct tatttgcact gactcgggaa acagccccac
      661 gtcacttccg aatcacagcg atttgaatcc agttttcccg cgctgtcgag agtcaacgat
      721 tcgttaatga aaataaatct tgtccaacgc ggaatcgaag ctctgaaagt gagtccgaaa
      781 cgcgaacgcg cccgtaattc tacgcgtagg gcggctcggc atggctgatg acgcgcttca
      841 cggtgggaaa cgcgcggcgc agcgcccgct cgatctcgtc gacattctcg tggaccttga
      901 tcacgctcat cgacggcgcc gcgcggcaat ggaagttcac gatttcgccg gcctcggtat
      961 ttcgcacccg cacattatgg atgtcgtgga cggcgccttc ggcggcaaag cgcgtcagcg
     1021 cgctcttgat gaccgcgacg cggtcgggcg cggcgtcgct gcccagcggc aattcgggct
     1081 ccagcggctc gatatgggtg tcgacctcga cgtcctcgcc gaattcatgg cggatgttgc
     1141 tttcgagatc atgagcgatg tcgtgggctg atgtcagtgc catctccccg tcgacttcga
     1201 ggtcgatact gacggtcagc ctgtcgccga gatcgtgcac ggtgacatgg tgaacggcga
     1261 ggccggaatt gcgggcgatc accatgatgc gctcgcgcac gctttcattg tcgcgggcga
     1321 ccggcacggc ggtgaaggtc aggtcggcgt cgtcgaacac ctgcgagacg cccgcctgtg
     1381 ccttgcgctt gatcgcgtcg acgcgatcga tcgggaatgt gcggggtacc tgcacgatgg
     1441 cgtcgatgaa ataggtcggg ccgaccatgc ggacgcgcaa gcgctcgacg tcgatcacgc
     1501 cgggcacggc cttgatggcc tcgatagcct tttccgacac gccctcgggc gcgcggtcga
     1561 ccagcgtttc cacggtggag cgtccaagcc gcaaccccaa caccgagatc ataatggcga
     1621 cgccgatcgc cgcggccgag tcgccccagg cgtagcccag tcccgacagc gccagcccga
     1681 agatcacggc gatcgagccc agcacgtcgg atgcaaaatg tagcgcatcg gcggcgagcg
     1741 cctggctcct tgtatcgcgc gcggcgcgat gcagcgcccg ggcgcgccag aaattcacgg
     1801 cgatgtcgat cacgagcacc acgaagggga tcgcggacaa agttggcggc ggcgcgccct
     1861 cgcgcagacg gctgacggat tcgaccagaa tgccgccggc cagcacatag agcagggcga
     1921 tgacgccgag tgccgacagg ctctcgagct tgccgtggcc gtagtgatgc tcctcgtcgg
     1981 cggggcggtc ggaaacccgt accaccagcc aggtgatgac ggtcgcgacg acgtcgaccg
     2041 acgagtgcag cgcctcggag atcagcgcga gactaccgat ggcgatgccg accgtgaatt
     2101 tggccgcggc catgccggcg ctggcaaaaa tcgaaatcgc cgcga
//
LOCUS       DATMJB010000459         3810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_554688, whole genome shotgun sequence.
ACCESSION   DATMJB010000459 DATMJB010000000
VERSION     DATMJB010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554688"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1642)
                     /locus_tag="VNU48_09820"
     CDS             complement(107..1642)
                     /locus_tag="VNU48_09820"
                     /inference="COORDINATES: protein motif:HMM:NF036848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11615.1"
                     /translation="MLDNAARRIPVIVGVGQLNDRSDDDAAALDTLALMAAALHLADH
                     DAGGGFLGQLQSLGIVEQMSWPTASWPGPNKITPYLLAALGIAPSHVELTPEPSGDSP
                     LRLLNDAANRIGTGEVEVAAVVGGEALRTAARRAATLGAPQQDLIRTVTEQRAGPLKK
                     QYGLLTATDVYPLYENACRAAWGQSLAQAQAETAAIWAGLSAAAADNPDAWIRRRFDP
                     AELLAASADNRPVSFPYAKLMVANSSVNQGAAVIVASLAKAREMGVPDAQLVYLGPGA
                     SAHEPEDFLARDRYDASPSMAVSIQRALALNGLAASDLDLVELYSCFPCIPKMARRIL
                     GWPLERRHSVYGGLTFGGGPIANCMTHAVVTMTRMLRHAGRHGLIFANGGYATHHHTL
                     VLTREPVPAGRFPQRHEHQAEADALRGPVPALIEGYTGPGVVETYMLPYDRAGRPQFS
                     TVIARTPSGQRFLCRVLPNDGATLAALTSGEREPVGRHGVASHGAGGHVDWRFSEMPR
                     SGP"
     gene            complement(1652..2452)
                     /locus_tag="VNU48_09825"
     CDS             complement(1652..2452)
                     /locus_tag="VNU48_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWI11616.1"
                     /translation="MTTPLAPQGADEPILFERIARHIARVTLNRPQARNAVNVAMTLR
                     LEEVVRSIEADPELRVAILAAAPGPAFCAGADLAEVAAGRGPSLVTREGGFAGFVYAT
                     RHKPWIAAVHGFVLGGGLEFALACDMVVAAQDAVFGLPEPKRGLLAAAGGAYRLPRAV
                     PRAIALEMLSTGATLDAQRAHALGLVNRVVDLAALPEAALALAAQVVANEPLSVRESL
                     AIGRAAAELDEAALRRLSAEATARVMASEDAREGPRAFVEKRAPVWRT"
     gene            complement(2449..>3810)
                     /locus_tag="VNU48_09830"
     CDS             complement(2449..>3810)
                     /locus_tag="VNU48_09830"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWI11617.1"
                     /translation="GATELTAQVAAAHTGALVGNDAVFDAACRQLGVLRVHTLEDLVV
                     TAGLLAHTGPLRAPGVGIVSISGGACTLIADRAEAHGVSLPALSPATRAALREAVPGP
                     GEPLNPFDITGLAVRDPALFERVLGALGNDPAIGFVGCVYSMPWNDKWQQVPAIEAIG
                     RALATLGKPSAMLNQTLRPVSQKSRDIMAACGVPAAFGGLEAVVAALGQISRWSAWLE
                     AQATREQPLPVLPATARPDSERATLDFLARRGVPVIPARLATTRGEAVDFAASLAAPV
                     AMKIASPDIAHKTEVGGVRLDLQGDAAVGAAFDAMLAGVRRVRPDARLDGVLVSPMRE
                     GGLEILVGTVRDPQWGLTLAVGLGGIWVEALADTQLRLLPVTPQDARDMLTRLRAAKL
                     LQGFRGSPPVDLDRLARVIAAIGDAALALGDSLAALEVNPLWVRGDAIEALDALAVWG
                     DAP"
BASE COUNT          533 a         1379 c         1407 g          491 t
ORIGIN      
        1 gagcgtcatc gcgcgcaatt cgggatccca ttgctccacg ggcagcttgg ctactcgact
       61 catcgtcgtc tcctggttgt cgacaaaaaa actcttgcag cgcggttcac ggcccgctgc
      121 gcggcatctc cgaaaagcgc cagtccacat ggccgccggc gccatgcgag gccacgccgt
      181 ggcggcccac cggttcacgt tcgcccgagg tcagcgcagc cagggtcgcg ccatcgttag
      241 gcagaacgcg gcagaggaag cgctggccgc tcggcgtgcg ggcgatgacg gtggaaaact
      301 gcggccgccc ggcgcggtcg tagggcagca tgtaggtctc caccaccccc gggccggtgt
      361 agccctcgat caaggccggc accggaccgc gcagtgcatc ggcctcggcc tggtgctcgt
      421 ggcgctgcgg aaagcggccc gccggcaccg gctcgcgcgt cagcacgagc gtgtggtggt
      481 gggtggcata gccgccgttg gcgaagatca gcccgtggcg gccggcatgg cgcagcatcc
      541 gcgtcatcgt gaccacggcg tgcgtcatgc agttggcgat cgggccgccg ccgaaggtca
      601 agccgccgta gacgctgtgg cgccgctcca gcggccagcc aaggatgcgc cgcgccatct
      661 tcggaatgca gggaaagcag ctgtacagct cgacgaggtc gagatcgctc gcggccaagc
      721 cattcaaggc cagggcacgc tggatcgata ccgccatgct gggcgaggcg tcgtagcggt
      781 cgcgcgccag gaagtcctcg ggctcgtgcg cggaggcgcc aggcccgaga tagaccagtt
      841 gcgcatcggg cacgcccatc tcgcgcgcct tggccaggct ggcgacgatc accgccgcgc
      901 cctggttgac gctgctgttg gccaccatca gcttggcgta ggggaacgag acgggccggt
      961 tgtcggccga cgcggcgagc agttcggccg gatcgaagcg acggcggatc caggcatccg
     1021 ggttgtcggc cgcggcggcg gacaggccgg cccagatcgc cgccgtctcg gcctgcgcct
     1081 gcgccaggct ctggccccag gcggcgcggc aggcgttttc atagagcggg tacacatcgg
     1141 tggcggtgag caggccgtat tgcttcttca gcgggccggc gcgctgctcg gtcacggtgc
     1201 ggatcaggtc ctgctgcggc gcccccaggg tcgcggcgcg gcgcgccgcc gtgcgcaagg
     1261 cctcgccccc gaccaccgcg gccacctcca cctcgccggt gccgatgcgg ttggcggcgt
     1321 cgttgagcag gcgcagcggg ctgtcgccac tgggctccgg cgtcagctcg acatgggacg
     1381 gcgcgatccc cagcgcggcc agcaggtagg gcgtgatctt gttcggaccc ggccaagagg
     1441 ccgtcggcca cgacatctgc tcgacgatgc ccagggattg cagctggccc agaaaaccgc
     1501 cgccggcatc atggtcggcg aggtgcagcg ctgcggccat cagcgccagt gtgtcgagcg
     1561 cggcggcatc gtcatcggac cgatcgttga gctggcccac gccgacgatg acggggatgc
     1621 gccgcgcggc gttgtcaagc atggccatgg ctcaggtgcg ccacaccggc gcacgcttct
     1681 cgacgaaggc gcgcgggcct tcgcgggcgt cttccgaggc catcacccgc gccgtcgcct
     1741 cggcgctgag ccggcgcaag gccgcctcgt ccagctcggc ggcggcgcgg ccgatggcca
     1801 ggctctcgcg caccgacagc ggctcgttgg cgaccacctg ggcggccagg gccagggccg
     1861 cctccggtag cgcggcgagg tcgacgacgc gattgaccag gccgagtgca tgcgcccgct
     1921 gcgcgtcgag ggtggcgccg gtgctcagca tctcgagcgc gatggcgcgc ggaacggcgc
     1981 gcggcaggcg gtaggcgccg ccggccgcgg ccagcaagcc gcgcttgggt tcgggcaagc
     2041 cgaacacggc gtcctgcgcc gccaccacca tgtcgcaggc gagcgcgaac tccagccccc
     2101 cacccaacac gaagccgtgc acggcggcga tccacggctt gtgccgcgtc gcgtagacga
     2161 agccggcgaa cccgccttcg cgcgtcacca acgacgggcc gcggccggcc gcgacctcgg
     2221 ccaggtcggc cccggcgcag aacgccgggc ccggtgcggc ggcgaggatc gccacacgca
     2281 actcggggtc ggcttcgatg ctgcggacga cctcctccag gcgcagcgtc atcgcgacgt
     2341 tgacggcatt gcgcgcctgc gggcggttga gcgtcacccg cgcgatgtgg cgggcgatgc
     2401 gctcgaacag gatgggctcg tcggcgccct ggggtgcgag gggcgtcgtc atggcgcgtc
     2461 gccccacacc gccagtgcgt ccagcgcttc gatggcgtcg ccgcgcaccc acagcgggtt
     2521 gacctccaag gcggcgagcg agtcgcccag cgccagcgcc gcgtcgccga tggcggcgat
     2581 cacccgggcc aggcgatcca ggtccaccgg cgggctgccg cgaaagcctt gcagcagctt
     2641 ggcggcgcgc agacgggtga gcatgtcgcg tgcatcctgc ggcgtgaccg ggagcagtcg
     2701 cagctgcgta tcggccagcg cctcgaccca gatgccgccc agtccgacag ccagggtcag
     2761 cccccactgc gggtcgcgca cggtgccgac caggatctcc agcccgccct cacgcatcgg
     2821 cgagaccagc acgccgtcga gccgcgcgtc gggccggacc cgtcgcacgc cggccagcat
     2881 ggcgtcgaag gccgcgccga ccgccgcgtc gccctgcaga tcgagccgga cgccgccgac
     2941 ctcggtcttg tgcgcgatgt cgggcgacgc gatcttcatc gccaccggcg cagccagcga
     3001 cgcggcgaag tcgaccgcct cgccgcgcgt ggtcgcgagc cgggcgggga tcaccggcac
     3061 gccgcggcgg gccagaaagt ccagcgtcgc gcgttcgctg tcgggccgcg cggtggcggg
     3121 cagcaccggc aggggctgct cgcgcgtcgc ttgcgcttcg agccaggcgc tccagcggct
     3181 gatctggccc agcgccgcca ccaccgcctc caggccgccg aacgccgcgg gcacgccgca
     3241 ggcggccatg atgtcgcggc tcttctgcga caccggccgc agcgtctggt tgagcatggc
     3301 cgagggcttg ccgagcgttg ccagcgcgcg gccgatggcc tcgatcgccg gcacctgctg
     3361 ccacttgtcg ttccacggca tgctgtagac gcagccgacg aagccgatcg ccgggtcgtt
     3421 gccgagggcg ccgagcacgc gctcgaacag ggccgggtcg cgcaccgcga gaccggtgat
     3481 gtcgaaggga ttgagcggct cgcccggccc gggcacggcc tcgcgcaatg cggcccgggt
     3541 ggccggcgac aacgcgggca ggctcacgcc atgcgcctcg gcgcggtcgg cgatgagcgt
     3601 gcaggcgcca ccggaaatcg acacgatgcc gacgccgggc gcccgcagcg gcccggtgtg
     3661 ggccagcagc ccggcggtga cgaccaggtc ttcgagcgtg tgcacgcgca gcacgccgag
     3721 ctgccggcag gccgcgtcga acacggcatc gttgcccacc agcgcgccgg tgtgggctgc
     3781 ggccacctgg gccgtcagct cggtggcgcc
//
LOCUS       DATMJB010000460         6255 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_8342, whole genome shotgun sequence.
ACCESSION   DATMJB010000460 DATMJB010000000
VERSION     DATMJB010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6255
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8342"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="VNU48_09835"
     CDS             complement(<1..469)
                     /locus_tag="VNU48_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWI11618.1"
                     /translation="MSFDLVFFGGTGDLTWRKLVPALFQAFRHGKLPPDGRILAVARD
                     EQTDEQYRAWIKERFQNVEGAKRPSDDEFANFAEMLHYRRLDLSQPEHYARLKQWLNQ
                     RQADTVVLYLATSPYLFPQICTQLGAAGLNDPRIRVVLEKPLGHDLASAQEINR"
     gene            684..1637
                     /gene="tal"
                     /locus_tag="VNU48_09840"
     CDS             684..1637
                     /gene="tal"
                     /locus_tag="VNU48_09840"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HWI11619.1"
                     /translation="MNQLDQLKQFTTVVADSGDFRQLAQYAPRDATTNPSLILKAVGM
                     PEYAPLLKETVAAHRGEPLETIVDEVLVRFGLEILKIVPGRVSTEVDARLSFDTACSV
                     AKAHRLIAMYEAAGVARERILIKLASTWEGIQAARILQHEGINCNMTLLFSFCQAVCS
                     GDAGAKLISPFVGRIYDWYKKSAGSGWVEAERAGANDPGVRSVAQIYTYYKKFGVETE
                     VMGASFRNVGQIRALAGCDLLTISPELMRELQASDAPLTRALDPEVAKLVPIHAVTYN
                     EASFRFALNEDAMATEKLAEGIRTFAVDAGKLEKMIEALKS"
     gene            1634..3259
                     /gene="pgi"
                     /locus_tag="VNU48_09845"
     CDS             1634..3259
                     /gene="pgi"
                     /locus_tag="VNU48_09845"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HWI11620.1"
                     /translation="MNPRCDRTEAWAALAGHFEAHGRAFDLREAFARDPGRFDALGIA
                     APEVFADLSKNLVDTATLHFLLDLARECGVEARRDAMPRGDAINTTEGRAVLHTALRA
                     PRGRGPFSAEVHAVLDAMLAYVETVRDTAASGIKHIVNIGIGGSDLGPQMVVPALDAF
                     AHPGLTLHFVSNVDGHDIVPVLRQLEPARTLFIIASKTFTTQETMANAHAAKAWFEAN
                     GGTDTAKHFVATTTNVAAAAQFGITTTFGFWDWVGGRYSLWSSIGLPIALAIGADNFR
                     ALLAGAHEMDEHFAHAPAAKNLPLLLGLVDVWYRNFHRFTSRSVAPYHQGLKRLPAYL
                     QQLEMESNGKRVDLAGEALPYATSPVVWGEPGTNGQHAYFQMLHQGTDTIPVEFILVK
                     HATHGLAGLHGPLLAQLERQHAMLLANGLAQSQALMQGKTTEQARAESAPTASKELDP
                     PTLARHRTFPGNRPSTTLLLDQLTPGSLGALIALYEHRVFTSGALWGINSFDQWGVEL
                     GKALASDLLPRLASGDAGGLDASTAGLLARLRR"
     gene            3299..3934
                     /locus_tag="VNU48_09850"
     CDS             3299..3934
                     /locus_tag="VNU48_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014724383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HWI11621.1"
                     /translation="MVTNAELTFFHAPQTRSAGVLTLLEELGAPYTLHLVDMRAGEQR
                     QPEYLAINPMGKVPAIRHGDAIVTEQVAVFLYLADLFPQAGLTPAIGEPLRGPFLRWL
                     AFYGSSFEPAVIDRSQGHAPMPAMAPYGDYGTMLATLVAQLGRGPYILGERFSAADVL
                     WGTALTWTTMFGLVPQLPVIKAYVARTNARPAFVRARAKDEALLVSTLDRG"
     gene            complement(3947..5041)
                     /locus_tag="VNU48_09855"
     CDS             complement(3947..5041)
                     /locus_tag="VNU48_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HWI11622.1"
                     /translation="MSTDHVSANFSQTYQEARNKFLAEADVAGLDVHSHLHPMLGRDG
                     EQLALDVVRDGPADAAALLIVSSACHGVEGFCGSGVQTTLLQDRAWREAAADARVAVL
                     YLHALNPYGFSWWRRTTQENVDLNRNFHDFAGALPRNEAYDELAAAIVPPHWPPSAEV
                     EAVLQRYAAQHGVAKLQQAISGGQYAHPEGLFYGGRNPTWSHIALRHVLREHATRCAR
                     LGWIDLHTGLGPSGVGERIFACRDDAAALQRARAWWGDQVTSLYDGSSTSAPLQGLMW
                     LAAYEECPQAEYTGIALEYGTLPMMEVIGALRADQWIENHPEAPDALRAQIRQRTRDA
                     FYIDTDEWKQRIVEQAVDAAHGALRGLSAR"
     gene            complement(5038..6240)
                     /locus_tag="VNU48_09860"
     CDS             complement(5038..6240)
                     /locus_tag="VNU48_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HWI11623.1"
                     /translation="MRLIESILADAAAIAAVRRDIHAHPELCFQEQRTSDLIARQLTD
                     WGIPIHRGMGTTGVVAILKNGTSSRAVGLRADIDALPMTEHNTFAHASRHAGKMHACG
                     HDGHTAILLAAAKHLSKHRHYDGTVYLVFQPAEEGGGGAREMIKDGLFEKFPMEAIFG
                     AHNWPGMAVGQFALKSGPAFASSNEFKITIQGKGAHAAMPHLGLDPVPVACQLVQAFQ
                     TIITRNKRPIDAGVISVTMIHTGEATNVVPDSCEIQGTVRTFTLEVLDLIERRMQEIS
                     EHTCAAFGASCEFHFKRNYPPTINHPAETEFTRAVMTELVGADNVQTFEPTMGAEDFS
                     FFLLEKPGCYFLIGNGDGSHREGGHGMGPCMLHNPSYDFNDELIPLGATLWVKLAEQW
                     LNTARRPA"
BASE COUNT          922 a         2130 c         2219 g          984 t
ORIGIN      
        1 cgcggttgat ctcctgcgcg ctggccaggt cgtggccgag cggcttttcc agcacgacgc
       61 ggatgcgcgg atcgttcagg ccggcggcgc cgagctgcgt gcagatctgc gggaacaggt
      121 aggggctggt cgcgaggtag agcacgaccg tatcggcctg gcgctggttc agccactgct
      181 tcagccgcgc gtagtgctcg ggctgcgaca ggtccaggcg ccggtagtgc agcatctcgg
      241 cgaagttcgc gaactcgtcg tcgctcgggc gtttcgcgcc ttcgacgttc tggaagcgtt
      301 ctttgatcca ggcgcgatat tgttcgtcgg tctgctcgtc gcgcgcgacc gcgaggatgc
      361 gcccgtccgg cggcagcttg ccgtggcgga aggcctggaa cagcgccggc accagcttgc
      421 gccaggtcag gtcgccggtg ccgccgaaga agacgaggtc gaaggacatg tcggtcacgc
      481 gcacggccgg cgcgatgtaa cttagtttca ggcctgatga tgccgaagtt tcttccgccg
      541 gtcaacgcca ccgccatcac cttgaaccgt gtcggcgcga tggcggcgcg gggtactttc
      601 ggaaaacaag ctgaagccgg cggcgaacgg tggtcgtcgg atcgggttct aatcaggctt
      661 cgacgatctc tgccgcgccc gccatgaacc aactcgacca gctcaagcag ttcaccaccg
      721 tggttgccga cagcggcgac ttcaggcaac tggcgcagta cgcgccgcgc gacgcgacga
      781 cgaacccgtc gctgatcctg aaggcggtgg gcatgcccga gtacgcgccg ctgctgaagg
      841 agacggtcgc ggcgcaccgc ggcgagccgc tcgagaccat cgtcgacgag gtgctggtgc
      901 gcttcgggct ggagatcctg aagatcgtcc cgggccgggt ctcgaccgag gtcgacgcgc
      961 ggctgagttt cgacaccgcc tgcagcgtcg ccaaggcgca ccggctgatc gcgatgtacg
     1021 aggccgcggg cgtcgcacgc gaacgcatcc tgatcaagct cgcatcgacc tgggagggca
     1081 tccaggccgc gcgcatcctg cagcacgagg gcatcaactg caacatgacc ctgctgttct
     1141 cgttctgtca ggcggtgtgc agcggcgatg ccggggcgaa gctgatctcg cccttcgtcg
     1201 gccgcatcta cgactggtac aagaagagtg ccggcagcgg ctgggtcgag gccgagcgtg
     1261 ccggcgcgaa cgacccgggt gtcaggtcgg tcgcgcagat ctacacctac tacaagaagt
     1321 tcggcgtcga gaccgaggtg atgggtgcga gctttcgcaa cgtcgggcag atccgggcgc
     1381 tggccggctg cgacctgctg acgatcagcc ccgagctgat gcgcgagctg caggccagcg
     1441 acgcgccgct gacgcgtgcg ctcgaccccg aggtcgccaa gctcgtgccg atccacgccg
     1501 tcacctacaa cgaagcgagc ttccgtttcg cgctgaacga ggacgcgatg gcgaccgaga
     1561 agctcgccga gggcatccgc accttcgcgg tcgacgccgg caagctcgag aagatgatcg
     1621 aggcgttgaa gtcatgaatc caaggtgcga tcgcacggaa gcctgggccg cgctggccgg
     1681 ccacttcgag gcccacggcc gcgcgttcga cctgcgcgag gcgttcgcgc gcgaccccgg
     1741 ccgcttcgac gcgctgggca tcgcggcgcc cgaggtcttc gccgacctgt cgaagaacct
     1801 ggtcgacacc gcgacgctgc acttcctgct cgacctggcg cgcgaatgtg gcgtcgaggc
     1861 gcggcgcgac gcgatgccgc gcggcgacgc gatcaacacc accgagggcc gcgcggtgct
     1921 gcacacggcg ctgcgcgcac cgcgcggccg ggggccgttc agcgccgagg tgcacgcggt
     1981 gctcgacgcg atgctcgcgt atgtcgagac ggtgcgcgac accgccgcga gcggcatcaa
     2041 gcacatcgtc aacatcggca tcggcggcag cgacctcggg ccgcagatgg tggtgccggc
     2101 gctcgatgcg ttcgcacacc cgggcctgac gctgcacttc gtctcgaacg tcgatggtca
     2161 cgatatcgtg ccggtgctgc gccagctcga gccggcgcgc acgctgttca tcatcgccag
     2221 caagaccttc acgacccagg agacgatggc caacgcgcac gccgcgaagg cctggttcga
     2281 ggcgaacggc ggcaccgaca ccgcgaagca cttcgtcgcg acgacgacga acgtggccgc
     2341 cgcggcgcag ttcggcatca cgacgacctt cggcttctgg gactgggtcg gcgggcgcta
     2401 ctcgctgtgg tcgtcgatcg ggttgccgat tgcgctggcg atcggcgccg acaacttccg
     2461 cgcgctgctg gccggcgcgc acgaaatgga cgagcacttc gcgcacgcgc cggcggcgaa
     2521 gaacctgccg ctgctgctcg gcctcgtcga cgtctggtat cgcaacttcc accgcttcac
     2581 gagccgcagc gtcgcgccct accaccaggg cctgaagcgg ctgcccgcgt atctgcagca
     2641 gctcgagatg gagagcaacg gcaagcgcgt cgacctggcc ggcgaggcgc tgccgtacgc
     2701 gaccagcccg gtcgtctggg gcgagccggg cacgaacggc cagcacgcgt acttccagat
     2761 gctgcaccag ggcaccgaca cgatcccggt cgagttcatc ctcgtcaagc atgcgacgca
     2821 cgggctggcc gggctgcacg gcccgctgct cgcgcaactc gagcgccagc acgcgatgct
     2881 gctcgccaac ggcctggcgc aatcgcaggc gctgatgcag ggcaagacga ccgagcaggc
     2941 gcgcgccgaa agcgcgccga ccgcgtcgaa ggaactcgac ccgccgacgc tcgcgaggca
     3001 ccgcacgttt cccggcaacc ggccgagcac gaccctgctg ctggatcagc tgacgccggg
     3061 ctcgctgggc gcgctgatcg cgctgtacga gcaccgcgtc ttcaccagcg gggcgctgtg
     3121 gggcatcaac agcttcgacc agtggggcgt ggagctcggc aaggcgctgg ccagcgacct
     3181 gctgccgcgg ctggcgtcgg gcgatgcggg cgggctcgat gcctcgacgg ccggcttgct
     3241 cgcgcggctg cggcgctgac ccctcgaccg acgaccgacc gacgaaagga ccgacccgat
     3301 ggtgacgaac gctgaactga ccttcttcca cgcgccgcaa acgcgctcgg ccggcgtgct
     3361 caccctgctc gaggagttgg gcgcgccgta cacactgcac ttggtcgaca tgcgggcggg
     3421 tgagcagcgc cagcccgagt acctggcgat caacccgatg ggcaaggtgc ccgcgatccg
     3481 ccacggcgac gcgatcgtca ccgagcaggt cgcggtcttc ctctacctgg ccgacctgtt
     3541 tccccaggcc ggcctcacgc ccgcgatcgg cgagccgctg cgcgggccgt tcctgcgctg
     3601 gctcgcgttc tacggttcga gcttcgagcc ggcggtgatc gacagatcgc agggtcatgc
     3661 gccgatgccg gcgatggcgc cgtacggcga ctacggcacg atgctggcca cgctggtcgc
     3721 ccagctcggg cgcgggccgt acatcctggg cgagcgcttc agcgctgccg acgtgctgtg
     3781 gggcaccgcg ctgacctgga ccacgatgtt cgggctcgtg ccccagctgc ccgtgatcaa
     3841 ggcttacgtc gcccgcacca acgcgaggcc ggcgttcgtg cgtgcgcgtg cgaaggacga
     3901 ggcgctgctc gtgtcgacac tggaccgcgg gtagggccgc gcgccgtcag cgcgccgaca
     3961 gcccgcgcag cgcgccgtgc gccgcgtcga cggcctgctc gacgatgcgc tgcttccact
     4021 cgtcggtgtc gatgtagaac gcgtcgcgcg tgcgctgcct gatctgcgcg cgcaaggcgt
     4081 cgggggcctc ggggtgattc tcgatccact ggtcggcgcg cagcgcgccg atcacctcca
     4141 tcatcggcag cgtgccgtat tcgagcgcga tgccggtgta ctcggcctgc gggcattcct
     4201 cgtaggcggc gagccacatc aggccctgca gcggcgccga ggtcgagctg ccgtcgtaga
     4261 ggctggtgac ctggtcgccc caccaggcgc gcgcgcgctg caacgcggcg gcgtcgtcgc
     4321 ggcacgcgaa gatgcgctcg ccgacgccgc tcggcccgag gccggtgtgc aggtcgatcc
     4381 agcccaggcg ggcgcagcgc gtcgcgtgct cgcgcagcac gtggcgcagc gcgatgtggc
     4441 tccaggtcgg gttgcggccg ccgtagaaca ggccctcggg gtgcgcgtac tggccgcccg
     4501 agatcgcctg ctgcagcttc gccacgccgt gctgcgcggc atagcgctgc agcaccgcct
     4561 cgacctcggc cgacggcggc cagtgcggcg gcacgatcgc ggcggcgagt tcgtcgtagg
     4621 cctcgttgcg cggcagcgcg ccggcgaagt cgtggaaatt gcggttcagg tcgacgttct
     4681 cctgcgtggt gcggcgccac cacgagaaac cgtacgggtt cagcgcgtgc aggtacagca
     4741 ccgcgacgcg cgcatcggcg gcggcctcgc gccacgcgcg gtcctgcagc agcgtggtct
     4801 gcacgcccga accgcagaag ccttcgacgc cgtggcaggc gctgctgacg atcagcagcg
     4861 cggcggcgtc ggcggggccg tcgcgcacca cgtccagcgc gagctgctcg ccgtcgcggc
     4921 ccagcatcgg gtgcaggtgg ctgtgcacgt cgagcccggc gacatcggcc tcggcgagaa
     4981 acttgttgcg cgcttcctgg taggtctgcg agaagttggc gctgacgtga tcggtgctca
     5041 tgccggtcgg cgcgccgtgt tcagccactg ctcggccagc ttgacccaca gcgtcgcgcc
     5101 cagcgggatc agctcgtcgt tgaagtcgta gctcgggttg tgcagcatgc acgggcccat
     5161 gccgtggccg ccttcgcggt ggctgccgtc gccgttgccg atcaggaagt agcagccggg
     5221 cttttcgagc aggaagaagc tgaagtcctc ggcgcccatc gtcggctcga acgtctgcac
     5281 gttgtcggcg ccgaccagct cggtcatcac cgcacgggtg aattcggtct cggcggggtg
     5341 gttgatggtc ggcgggtagt tgcgcttgaa gtggaactcg caactcgcgc cgaacgcggc
     5401 gcaggtgtgc tcgctgatct cttgcatgcg ccgctcgatc aggtccagca cctcgagcgt
     5461 gaaggtgcgc accgtgccct ggatctcgca gctgtcgggc acgacgttgg tcgcctcgcc
     5521 cgtgtggatc atcgtcaccg agatcacgcc ggcgtcgatc ggccgcttgt tgcgcgtgat
     5581 gatggtctgg aacgcctgca ccagctggca cgccaccggc accgggtcga gccccaagtg
     5641 cggcatcgcc gcgtgcgcgc ccttgccttg gatcgtgatc ttgaattcgt tgctgctggc
     5701 gaaggccggg ccgctcttca gcgcgaactg gcccaccgcc atgccgggcc agttgtgcgc
     5761 gccgaagatc gcctccatcg ggaacttctc gaacaggccg tccttgatca tctcgcgcgc
     5821 gccgccgccg ccttcttcgg ccggctggaa cacgaggtag acggtgccgt cgtaatggcg
     5881 gtgcttgctg aggtgcttgg ccgcggccag cagtatcgcg gtgtggccgt cgtggccgca
     5941 ggcgtgcatc ttgccggcgt gccggctggc gtgcgcgaag gtgttgtgct cggtcatcgg
     6001 cagcgcgtcg atgtcggcgc gcaggccgac cgcgcgcgaa ctcgtcccgt tcttcaggat
     6061 cgcgaccacg ccggtcgtgc ccatgccgcg gtggatcggg atgccccagt cggtgagctg
     6121 ccgggcgatc aggtccgacg tgcgttgctc ctggaagcac agttccgggt gggcatggat
     6181 gtcgcggcgc accgcggcaa tcgccgcggc gtcggcgagg atggattcga tcaggcgcat
     6241 ggcaggctcc ggggc
//
LOCUS       DATMJB010000461         4848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_423115, whole genome shotgun sequence.
ACCESSION   DATMJB010000461 DATMJB010000000
VERSION     DATMJB010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423115"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VNU48_09865"
     CDS             <1..1063
                     /locus_tag="VNU48_09865"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003070968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HWI11624.1"
                     /translation="TATAAVDKGEPVATARRVKAADKRIINGQTDVNQLVPFKYKWAW
                     EKYLATCANHWMPQEVNMSRDIATWKDPNGLTDDERRIIKRNLGFFVTADSLAANNIV
                     LGTYRHITAPECRQFLLRQAFEEAIHTHAYQYIVESLGLDESEIFNAYNEIKSIRDKD
                     EFLLPFIGEIMDPNFHTGTPENDRTLLKSLIVFACLMEGLFFYVGFTQILALGRQNKM
                     TGAAEQYQYILRDESMHCNFGIDLINQIKLENPHLWTVEFKAEITALFREAVELEYRY
                     AEDTMPRGVLGMNASMFKGYLRYIANRRATQIGLEELFPNEENPFPWMSEMIDLKKER
                     NFFETRVIEYQSGGALSWD"
     gene            1315..1701
                     /locus_tag="VNU48_09870"
     CDS             1315..1701
                     /locus_tag="VNU48_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone H1-like repetitive region-containing
                     protein"
                     /protein_id="HWI11625.1"
                     /translation="MATAKKAPAKKAAAKKSPAKKAVTKKAPAKKAAAKKAPAKKAAA
                     KKAPAKKAPAKKAAAKKAPAKKAAAKKTPAKKAAAKKAPAKKAAAKKAKKPAAKKSAA
                     AAAPVQPAKTSLSPQAAWPFPTGSKP"
     gene            complement(1755..2675)
                     /locus_tag="VNU48_09875"
     CDS             complement(1755..2675)
                     /locus_tag="VNU48_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HWI11626.1"
                     /translation="MSALICGSLAFDTIATFPGRFAQQILPEQLHILNVSFLVPTLRR
                     EFGGCAGNIAYSLSRLGGQPMIMAAVGADGADYLRRIESWGASTSLIRTIADTHTAQA
                     IIITDTDNNQITAFHPGAMQSAHLGAVPTDPEIRVAIIAPDGREAMLQHAAQLAQASI
                     PFIFDPGQGLPMFDGAELRSFVDRAAWVAVNDYEARMLCERTGQTLASMSNSHLRGIV
                     VTLGANGCDLWQRGERTRVPGIHVDAVVDPTGCGDAFRAALLYGIEQGWPLERSVALG
                     NRIGAVKVGCRGGQNHPVDLSSLDAQFGEQ"
     gene            complement(2728..3693)
                     /locus_tag="VNU48_09880"
     CDS             complement(2728..3693)
                     /locus_tag="VNU48_09880"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HWI11627.1"
                     /translation="MSLATRCTACGTVFRVVQDQLKVSEGWVRCGRCSDVFNALEGLF
                     DLDRDAPPEGLETRPPAAAMTGAGAAPTITAPLQAASLLEKIDAQLLGPRDGEHVSAP
                     AIHDRDRLEFPDAQFGPDLGDDEMAMPPSMVAATGREDLDDEGAASDAAPEFLQRAQR
                     QARWQSPRMRALQAASVVILIALLGLQAVHHHRDIVAARWPATAASLTAWCGLMACAI
                     EAPRRIEDVAVESTALTRALPGQDAFNLSVVLRNRGTTAVAQPSIDLSLTDAGGQIIA
                     RRMLSAREFRVASASIKPGAETTLQLALSAGAARVSGYTVEIFYP"
     gene            complement(3714..4616)
                     /gene="prmA"
                     /locus_tag="VNU48_09885"
     CDS             complement(3714..4616)
                     /gene="prmA"
                     /locus_tag="VNU48_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HWI11628.1"
                     /translation="MVELLLLAPQDCVEDVGDALIDELDALSVSVEDADADTTAERAL
                     FGEPGLPAPRAGWERSMIRALFADDGAATEAATRLLAQDWAQAVHVQAIRAVPDQDWV
                     RLTQSQFAPVPITPDFWIVPSWHEPPAGAKQVIRLDPGLAFGTGTHPTTRMCLRWIAQ
                     QPANRRARWARVLDYGCGSGILAIAAALHGAGQVDAVDIDPAAVESTRANAQANRVAL
                     NAGLPDGAAGRYELVFANILATPLTLLAPLLCAHVAVDGDLVLAGILDRQAEQLTTAY
                     APWLALQIADSQDGWILMTAHRPA"
     gene            complement(4632..>4848)
                     /locus_tag="VNU48_09890"
     CDS             complement(4632..>4848)
                     /locus_tag="VNU48_09890"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904757.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HWI11629.1"
                     /translation="PNYDSMIGKIIVHGDTRDQALARMRIALSETVIEGIQTNVALHR
                     ELMVDAKFIEGGTSIHYLEGWLGQHKR"
BASE COUNT          878 a         1603 c         1620 g          747 t
ORIGIN      
        1 gactgccacg gcagccgtcg acaagggcga gcccgtcgcg accgcgcggc gcgtgaaggc
       61 cgccgacaag cgcatcatca acggccagac cgacgtcaac cagctggtcc cgttcaagta
      121 caagtgggcg tgggagaagt acctcgccac ctgcgcgaat cactggatgc cgcaagaggt
      181 caacatgtcg cgcgacatcg cgacctggaa ggacccgaac ggtctgaccg acgacgagcg
      241 ccgcatcatc aagcgcaacc tcgggttctt cgtcaccgcc gactcgctgg ccgcgaacaa
      301 catcgtgctg gggacctatc ggcatatcac ggcccccgag tgccgccagt tcctgttgcg
      361 ccaggcgttc gaggaagcga tccacacgca cgcgtatcag tacatcgtcg agtcgctggg
      421 gctggacgaa agcgagatct tcaacgccta caacgagatc aagtcgatcc gcgacaagga
      481 cgagttcctg ctcccgttca tcggcgagat catggacccg aatttccaca ccggaacgcc
      541 ggagaacgac cggacgctgc tgaagtcgtt gatcgtgttt gcgtgcctga tggaagggct
      601 gttcttctat gtcgggttca cgcagatcct ggcgctgggc cgtcagaaca agatgaccgg
      661 cgcggccgag cagtaccagt acatcctgcg cgacgagtcg atgcactgca acttcggcat
      721 cgacctgatc aatcagatca agctcgagaa cccgcacctg tggacggtcg agttcaaggc
      781 cgagatcacc gcgctgttcc gcgaggcggt cgagctcgag taccgctacg cggaagacac
      841 gatgccgcgc ggcgtgctcg gcatgaatgc atcgatgttc aagggctatc tgcgctacat
      901 cgccaaccgg cgtgcgacgc agatcggcct ggaagaattg ttcccgaacg aggagaaccc
      961 gttcccctgg atgagtgaaa tgatcgacct gaagaaggag cgcaatttct ttgagactcg
     1021 ggtgatcgag tatcagagtg ggggcgcgct ttcctgggat tgatctggtc cgcgcgcgcc
     1081 agtgcgtgcg cgggtcatcg agcctttcgt gccggtccgg acgaaagcaa gaagagtgat
     1141 caagatttgc gatgcgtgat gcgccgacaa agaagcggtg cactcgggtc gctactggat
     1201 tcatcgtact ggggcgcccc tcctgggcag attccggacg gtgaatagct gttgcgcaaa
     1261 ggctttggcc gatgtggagc agttcttttt gcaacttgat caaggagaaa cgaaatggca
     1321 actgcaaaga aagctccggc aaagaaggcc gcggcgaaga agtccccggc caagaaggcc
     1381 gtgaccaaga aggcgccggc caagaaggcg gcggccaaga aggcgcctgc gaagaaggcg
     1441 gccgccaaga aggcgcctgc gaagaaggcg ccggccaaga aggcggccgc caagaaggca
     1501 cctgcgaaga aggcggcggc caagaagacg cctgcgaaga aggcggcggc caagaaggcg
     1561 cctgcgaaga aggcggccgc caagaaggcc aagaagcccg cggcgaaaaa gagcgcagcc
     1621 gcggctgcgc cagtccagcc ggcgaagact tcgttgagcc cgcaggccgc ttggccgttc
     1681 ccgaccggaa gcaagccctg acggtcttgg gctgagtcac acggcccggc gcacgccggg
     1741 ctgtgtgctt ttgatcactg ttctccgaac tgggcgtcca gtgagctgag gtctaccggg
     1801 tggttctgac ctccccggca gcccaccttg accgcgccga tccggttccc caacgcgacg
     1861 cttcgttcga gcggccaacc ctgctcgatg ccgtacagca gggctgcacg aaaggcgtcc
     1921 ccgcatccgg tcggatcgac gacggcatcg acgtgaatcc ccggaacgcg cgtgcgctcg
     1981 ccgcgttgcc acaaatcgca gccattggca ccgagcgtca cgacgattcc gcgcaaatgc
     2041 gagttcgaca tcgacgccag cgtctgcccg gtccgctcgc acagcatccg ggcttcgtag
     2101 tcgttgacgg cgacccacgc ggcacgatcg acgaaactcc gcaactcggc gccgtcgaac
     2161 atcggcagtc cctggccggg gtcgaagatg aacgggatgc tcgcctgcgc cagttgcgcg
     2221 gcatgctgca gcatcgcttc ccggccgtcc ggcgcgatga tcgccacgcg aatctcggga
     2281 tccgtcggca ccgcgccgag gtgagccgac tgcatcgcac ccgggtgaaa ggcggtgatc
     2341 tggttgttgt cggtgtcggt gatgatgatg gcctgtgccg tgtgcgtgtc cgcaatcgtg
     2401 cggatcaagg acgtgctggc cccccacgat tcgatgcggc gcaggtagtc ggcgccgtcc
     2461 gcgccgaccg ccgccatgat catcggctgc ccgccgaggc ggctcagcga gtaggcgatg
     2521 ttgccggcgc aaccgccgaa ctcccgccgc agcgtcggca cgaggaacga cacgttcagg
     2581 atgtgcagct gttccggcag gatctgctgc gcgaagcgac cggggaaggt tgcgatcgtg
     2641 tcgaaggcga gcgaaccgca aatcaatgct gacatgaaag ctccagagtt gaactcgagg
     2701 tgaccgcggc ggggcaccgc cgctgggtca gggatagaag atctcgacgg tgtagccgct
     2761 gacgcgcgca gcgccggcgc tcagtgccaa ttgaagggtg gtttccgcgc ccggcttgat
     2821 cgatgcggat gcgacgcgaa actcgcgtgc cgagagcatg cgtcgcgcga tgatctgccc
     2881 gcccgcgtcg gtcaggctca ggtcgatgga cggctgcgcg accgcggtcg tgccgcgatt
     2941 gcgcagcacc accgacaggt tgaacgcgtc ctggccgggc agcgcgcgcg tcagcgccgt
     3001 gctctcgacc gccacgtctt cgatgcgccg cggcgcctcg atcgcacacg ccatcagccc
     3061 gcaccaggcg gtcagcgatg cggcggtggc gggccagcgc gccgcgacga tgtcgcgatg
     3121 atggtggacg gcctgcaggc cgagcagcgc gatcaggatc accaccgacg cggcctgcaa
     3181 cgcgcgcatc cgcgggctct gccaacgcgc ctgacgctgc gcacgctgaa gaaactccgg
     3241 cgccgcgtcc gacgcggccc cttcgtcgtc caggtcttcg cggcccgtcg ccgcgaccat
     3301 cgatggcggc atcgccatct cgtcgtcgcc caggtccggg ccaaactgcg catcggggaa
     3361 ctcgagccgg tcgcggtcgt ggatcgcagg tgctgaaaca tgctcgccgt cacgcgggcc
     3421 cagcagctgc gcgtcgatct tttccagcag cgacgcagct tgcagcggcg ctgtgatggt
     3481 cggcgcggca ccggcgcctg tcatggccgc ggcgggcggg cgggtctcga gaccctccgg
     3541 cggcgcgtcc cggtccagat cgaacagacc ttcgagcgca ttgaagacat cgctgcaacg
     3601 cccgcagcgc acccagcctt ccgacacctt gagctggtcc tgcaccaccc tgaagacggt
     3661 gccgcacgcg gtgcatcggg tggccaggct catgctcgaa gcgcttcgcg gggctacgcg
     3721 ggccgatgcg ccgtcatcag gatccagcca tcctgcgaat cggcgatctg cagggccagc
     3781 cagggcgcgt acgccgtcgt cagctgctcg gcctggcgat ccaggatgcc ggccagcacc
     3841 aggtcgccat cgaccgcgac gtgcgcacac agcagcggcg ccagcagggt caacggcgtg
     3901 gcgaggatgt tcgcgaagac cagctcgtag cggcccgccg caccatccgg cagcccggca
     3961 ttcagcgcga cgcgattcgc ctgcgcattc gcgcgcgtcg attcgaccgc cgccgggtcg
     4021 atgtcgaccg catcgacctg gccggctccg tgcagcgccg cagcgatcgc gagaatgccc
     4081 gagccgcagc cgtagtcgag cacgcgcgcc cagcgcgcac gccggttcgc tggctgctgt
     4141 gcgatccagc gcaggcacat gcgcgtcgtc gggtgcgtgc cggtgccgaa ggccaggccc
     4201 ggatcgaggc ggatgacctg cttcgcgccg gcgggcggct cgtgccagct gggaacgatc
     4261 cagaagtcag gggtgatcgg taccggtgcg aactgcgact gcgtcaagcg cacccagtcc
     4321 tggtccggga ccgcccggat tgcctgcacg tgcaccgcct gcgcccagtc ctgcgcgagc
     4381 agccgtgtcg ccgcctcggt ggccgcgccg tcgtcggcaa acagcgcgcg gatcatcgag
     4441 cgctcccagc ccgcgcgcgg cgccggcaga ccgggctcgc cgaacagcgc gcgctcggcc
     4501 gtggtgtcgg cgtcggcgtc ctcgaccgag accgacagtg cgtcgagctc gtcgatcaag
     4561 gcgtcgccga cgtcctcgac gcagtcctgc ggcgccagca gcagcagttc aaccatcggc
     4621 ggtccgtctt ttcaacgctt gtgctggccc aaccagcctt cgaggtagtg gatgctggtg
     4681 ccgccctcga tgaacttggc gtcgaccatc aattcccggt gcagcgcgac attcgtctgg
     4741 atgccctcga tcaccgtctc cgacagcgcg atgcgcatgc gtgccagcgc ctgatcacgg
     4801 gtatcgccgt gcacgatgat cttgccgatc atcgagtcgt agttcggc
//
LOCUS       DATMJB010000462         2767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_781710, whole genome shotgun sequence.
ACCESSION   DATMJB010000462 DATMJB010000000
VERSION     DATMJB010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781710"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /gene="rdgC"
                     /locus_tag="VNU48_09895"
     CDS             complement(<1..519)
                     /gene="rdgC"
                     /locus_tag="VNU48_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HWI11630.1"
                     /translation="MFKNLIVYRIGPGWAAHRAALEAGLEKSIFVPCGATQPVSAGWS
                     PPRGVAHGPLVEAAGAQWLLTLTIEQRVLPSAVVRRRVEERATQIEQASGRKPGKKQA
                     KELKEEIVLELLPQAFTKQATVKVWLNPKQRLLMVDASSPAKADEVVTHLVKALDGLG
                     LAPLHTTQSPAAA"
     gene            758..1681
                     /gene="rpoH"
                     /locus_tag="VNU48_09900"
     CDS             758..1681
                     /gene="rpoH"
                     /locus_tag="VNU48_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HWI11631.1"
                     /translation="MNTSSTAALTVRDPWAMVPSLGNLDAYITAANRVPLLTHEEEIA
                     YATRLRATGDLEAAGKLVLSHLRLVVSISRKYLGYGLPHGDLIQEGNIGLMKAVKRFD
                     PDQGVRLVSYAMHWIRAEIHEYILKNWRMVKVATTKAQRKLFFNLRSMKQSLKDDAAD
                     VDTHRSTLTQGEVDTLARTLNVKREEVLEMETRLSGGDVALEPQSDDSEESFAPIAYL
                     ADEASEPTRVLEARDRDWLAGDGIALALDALDARSRRIVEERWLKVNDDSSGGMTLHD
                     LAAEYGISAERVRQIEVAAMKKMRKALAESA"
     gene            complement(1736..2347)
                     /locus_tag="VNU48_09905"
     CDS             complement(1736..2347)
                     /locus_tag="VNU48_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWI11632.1"
                     /translation="MNTICRSALLAVALSAALVGCEKPGASSPPAVAFRGADITGAEY
                     ARTLALPDVNGQPRTLGDFKGKVTVVFFGYTQCPDVCPTTMTELAQVKKSLGVDGDKL
                     QAVFVTIDPERDTPEVLKSYMASFDPSFVALRGTLEQTQAAAKEFKVYFAKVPGKTEG
                     SYTMDHSAGAFVLDTRGNVRLFERYGADAEGLSADVKALIAQG"
     gene            complement(2344..>2767)
                     /locus_tag="VNU48_09910"
     CDS             complement(2344..>2767)
                     /locus_tag="VNU48_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWI11633.1"
                     /translation="LGWAAIRGDVGPEALIMCLIIFLWTPPHFWALALYRAEDYRKSG
                     LPMLPVTHGAEFTRLHVFLYTLVLFAGALLPFIQGMSGVLYLVAALLLNAGFVGYGWR
                     LWRAYSDALARKTFRFSIVYLSLLFAALLVDHYLRPLL"
BASE COUNT          496 a          934 c          908 g          429 t
ORIGIN      
        1 cgccgcggcc ggcgactgcg tcgtgtgcag cggcgcgaga cccagcccgt cgagcgcctt
       61 gacgaggtgc gtgacgacct cgtcggcctt cgccgggctg cttgcgtcga ccatcagcag
      121 gcgctgcttc ggattcagcc agaccttgac cgtggcctgt ttcgtgaagg cctgcggcag
      181 cagctcgagc acgatctctt ccttcagctc cttcgcctgc ttcttgccgg gcttgcgacc
      241 gctcgcctgt tcgatctgcg tcgcgcgctc ctcgacgcgg cgccgcacca ccgccgaggg
      301 cagcacgcgc tgctcgatcg tcaacgtcag cagccactgc gcgccggccg cttcgaccag
      361 cgggccgtgc gccacgcctc gcggcggcga ccagccggcg gacaccggct gggtcgcgcc
      421 gcagggcacg aagatcgatt tttccagccc cgcttcgagc gcggcgcgat gggcggccca
      481 gccggggccg atgcgataga cgatcagatt cttgaacaaa caaacctctc ttcaggtcgg
      541 gcccggcaca gcgcaccggg cggggagcgc gattgtccac gcaaaaagcc ggctctccga
      601 tcggcatatt gttagcaaac gccaacttct gatcggccgt gcggcatcac acgctgtcct
      661 ttgacaatcg ccgcgatagc gagctccatc agcactctcg aaccatgagt gctaacattt
      721 gcggaactcg aaaggagtcc gcgtgtctga tgcctccatg aacacttcat ccactgctgc
      781 gctgaccgtg cgcgacccct gggcgatggt gccctcgctg ggcaacctgg acgcctacat
      841 cacggcggcg aaccgcgttc ctcttctcac acacgaggag gagatcgcct acgcgacgcg
      901 cctgcgcgcc accggcgacc tcgaagccgc cgggaagctg gtgctgtcgc atctgcggct
      961 ggtggtctcg atctcgcgca aatacctggg ctacggtctg ccgcacggcg acctgatcca
     1021 ggaaggcaac atcggcctga tgaaggccgt gaagcgcttc gaccccgacc agggcgtgcg
     1081 cctcgtcagc tatgcgatgc actggatcag ggccgagatt cacgagtaca tcctgaagaa
     1141 ctggcgcatg gtcaaggtcg cgacgaccaa ggcgcagcgc aagctgttct tcaatctgcg
     1201 ctcgatgaag caaagcctga aggacgacgc cgccgatgtc gacacgcacc gctcgacgct
     1261 gacgcagggc gaggtcgaca cgctagcgcg caccctgaac gtcaagcgcg aagaggtgct
     1321 cgagatggaa acccggctgt cgggtggcga tgtggcgctc gagccgcaaa gcgacgacag
     1381 cgaggagagt ttcgcgccga tcgcctacct ggccgacgag gccagcgagc cgactcgcgt
     1441 gctcgaggcg cgcgaccgcg actggctcgc cggcgacggc atcgcgctcg cgctcgatgc
     1501 gctggacgca cgcagccgcc gcatcgtcga ggagcgctgg ctgaaggtga acgacgacag
     1561 ctcgggcggc atgaccttgc acgatctcgc cgccgagtac ggcatcagcg ccgagcgggt
     1621 tcgccagatc gaggtcgcgg cgatgaagaa gatgcgcaag gcgctggccg agagcgcgtg
     1681 atgcggttga cgcgataaaa agggggcccc tttccgttgc gcaagctcgg gggtctcagc
     1741 cctgagcgat caatgccttc acgtcggcgc tcaggccttc ggcgtcggcg ccatagcgct
     1801 cgaacaggcg cacgttgccc ctggtgtcga ggacgaaggc cccggccgaa tgatccatcg
     1861 tgtagctgcc ttcggtcttg cccggcacct tggcgaagta gaccttgaac tccttcgcgg
     1921 cagcctgcgt ctgttccaag gtgccgcgca gcgcgacgaa gctcggatcg aaactggcca
     1981 tgtacgactt caggacttcc ggcgtgtcgc gctccgggtc gatcgtgacg aacaccgcct
     2041 gcagcttgtc gccgtcgacg ccgagcgact tcttcacctg ggccagttcc gtcatcgtcg
     2101 tcgggcagac atccgggcac tgtgtgtagc cgaagaacac caccgtgacc ttgcccttga
     2161 agtcgccgag cgtgcgcggc tggccgttca cgtcgggcag cgccagcgtg cgggcgtact
     2221 cggcgccggt gatgtctgca ccgcggaagg ccaccgcggg cggcgacgac gcgcccggct
     2281 tctcgcagcc gacaagtgcc gcgctgagcg cgacggcgag caacgcggat cggcaaatcg
     2341 tgttcacagc agcggcctca ggtagtgatc caccagcagt gcggcgaaca gcagcgacag
     2401 gtagacgatc gaaaagcgga aggtcttgcg cgcgagcgca tccgagtagg cgcgccacag
     2461 cctccagccg tagccgacga agccggcatt gagcagcagc gccgccacca ggtacagcac
     2521 gccgctcatg ccctggatga acggcagcag cgcgccggcg aacagcacca gcgtgtacag
     2581 gaacacgtgc agccgcgtga actcggcgcc gtgcgtgacc ggcagcatcg gcaggccgct
     2641 cttgcggtag tcctcggcac ggtacagcgc cagcgcccag aagtgcggtg gtgtccagag
     2701 gaagatgatc aggcacatga tcagcgcctc gggcccgaca tcgccacgga tcgcggccca
     2761 gcccagc
//
LOCUS       DATMJB010000463         2323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_431511, whole genome shotgun sequence.
ACCESSION   DATMJB010000463 DATMJB010000000
VERSION     DATMJB010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431511"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /gene="truA"
                     /locus_tag="VNU48_09915"
     CDS             <1..735
                     /gene="truA"
                     /locus_tag="VNU48_09915"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWI11634.1"
                     /translation="WQSQPDGQTVQDKLEAALARFADHPLRTVCAGRTDTGVHALNQV
                     VHLDTSIVRAESSWVRGTNRFLPPDIAVQWCQLTDESFHARYAALGRRYVYVLLESPV
                     RPAVEAGSVGWSFRALDEAAMQSAARQLVGEHDFSAFRSSECQAATPVKTIRSIAISR
                     RGAYWRFEFDANAFLHHMVRNLMGALLAVGAGVRDETWLATVLAARDRSLAAPTFPAD
                     GLYFVGPYYDPVHAIPHRTPAFDWLP"
     gene            748..1395
                     /locus_tag="VNU48_09920"
     CDS             748..1395
                     /locus_tag="VNU48_09920"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWI11635.1"
                     /translation="MNLRTRIKICGLTRAADVDAAVQAGADAVGFVFYAKSPRHVAVA
                     HAAELARRLPPFVTPVGLFVNASAAEIEAAVAAIPQLLLQFHGDESAQDCARAGRPFI
                     RAARVAPGFDLLDFASRFAQAQALLLDAHVDGYGGGGKVFDWSLVRAAVPLPVVLSGG
                     LHAGNVIHGIQLLRPWAVDVSSGVESAKGIKDADAIRRFCDAVREADARGAASVA"
     gene            1414..>2323
                     /gene="trpB"
                     /locus_tag="VNU48_09925"
     CDS             1414..>2323
                     /gene="trpB"
                     /locus_tag="VNU48_09925"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWI11636.1"
                     /translation="MSNYQQPDASGHFGPYGGSFVAETLVHALDELKRAYAEVQNDPA
                     FIEEFRYELKHFVGRPSPIYHAARLSRELGGAQIFLKREDLNHTGAHKVNNTIGQAML
                     ARRMGKPRVIAETGAGQHGVATATICARYGLECVVYMGSEDVKRQSPNVYRMNLLGAT
                     VVPVESGSKTLKDALNEALRDWVTNVENTFYIIGTVAGPHPYPTMVRDFQRVIGDECI
                     AQMPAMLAAQGITGEAEGRQPDVVVACVGGGSNAMGIFYPYIDHAGTRLIGVEAAGQG
                     LATGKHAASLSAGSPGVLHGNRTYLLQ"
BASE COUNT          348 a          818 c          788 g          369 t
ORIGIN      
        1 tggcagagcc agcccgacgg gcagacggtt caggacaagc tcgaggcggc gctggcgcgc
       61 ttcgccgatc acccgctgcg caccgtctgc gccgggcgca ccgacaccgg cgtgcacgcg
      121 ctgaaccagg tcgtgcacct cgacacgagc atcgtgcgcg ccgaatcgtc ctgggttcgt
      181 ggcacgaacc gcttcctgcc gcccgacatc gccgtgcagt ggtgccagct gaccgacgag
      241 tcgttccatg cacgttatgc ggcgctcggc cggcgctatg tctacgtgct gctcgagtcg
      301 ccggtgcgcc ccgcggtcga ggccggctcg gtgggctgga gctttcgcgc gctcgacgag
      361 gcggcgatgc aatcggccgc acgccagctg gtcggcgagc acgacttcag cgcgttccgc
      421 tcgtcggagt gccaggcggc cacgccggtc aagacgatcc gctcgatcgc gatttcacga
      481 cgcggcgcgt actggcggtt cgagttcgat gcgaacgcgt tcctgcacca catggtgcgc
      541 aacctgatgg gcgcgctgct cgcggtcggc gcgggcgtgc gcgacgagac ctggctcgcg
      601 accgtgctgg cggcccggga ccgcagcctg gccgcgccga cctttcccgc cgacgggctg
      661 tacttcgtcg gcccgtacta cgatccggtc catgcgatcc cgcaccgcac ccccgcgttc
      721 gactggctgc cctgagggcg cgcggccatg aacctgcgca cccgcatcaa gatctgcggc
      781 ctgacccgcg cggccgatgt cgacgccgcg gtccaggccg gcgccgatgc cgtcggcttc
      841 gtcttctacg cgaagagccc gcgccatgtc gccgtcgcgc acgctgcgga actggcgcgc
      901 cgcctgccgc cgttcgtgac gccggtcggc ttgttcgtga atgcgagcgc ggccgagatc
      961 gaggccgccg tcgcggccat cccgcagctg ctgctgcagt ttcacggcga cgagtcggcg
     1021 caggactgcg cccgcgcggg tcgcccgttc atccgcgcgg cgcgggttgc gcccggtttc
     1081 gatttgctag acttcgcgtc ccgattcgca caggcccaag cccttctgct cgacgcccat
     1141 gtcgacggat acggcggcgg cggaaaggtt ttcgattggt ctctcgttcg cgccgccgtg
     1201 ccccttccag tcgttttgtc tggtgggttg catgccggaa atgtgatcca cggcattcag
     1261 ctgctgcggc cctgggccgt tgacgtgagt tccggcgtcg agtccgcgaa aggcatcaag
     1321 gatgccgacg cgatccgccg gttctgcgat gccgtgcgcg aggccgatgc ccgcggcgcg
     1381 gcatcggttg cctgaacagt caccctggat cacatgtcga actatcaaca acccgatgcc
     1441 tcgggccact tcggcccgta tggcggcagc ttcgtcgccg agacgctggt ccacgcactg
     1501 gatgagctca agcgcgccta tgccgaggtg cagaacgacc cggccttcat cgaagagttt
     1561 cgctacgagc tgaagcactt cgtcggccgg ccgagcccga tctaccacgc ggcgcggctg
     1621 agccgcgaac tcggcggcgc gcagatcttc ctgaagcgcg aagacctgaa ccacaccggc
     1681 gcgcacaagg tcaacaacac gatcggccag gcgatgctcg cgcgccgcat gggcaagccg
     1741 cgcgtgatcg ccgaaaccgg cgccggtcag cacggtgtgg ccacggcgac gatctgcgcg
     1801 cgctacggcc tggagtgcgt ggtctacatg ggcagcgaag acgtgaagcg ccagtcgccg
     1861 aacgtctacc gcatgaacct gctcggcgcg accgtggtgc ccgtcgagag cggctcgaag
     1921 acgctgaagg acgcgctcaa cgaagcgctg cgcgattggg tcaccaacgt cgagaacacc
     1981 ttctacatca tcggcacggt ggccggcccg cacccctatc cgaccatggt gcgcgacttc
     2041 cagcgcgtga tcggcgacga gtgcatcgcg cagatgccgg ccatgctggc agcccagggc
     2101 atcaccggcg aggcggaagg caggcagccg gatgtggtgg tggcctgcgt cggcggcggc
     2161 agcaatgcga tgggcatctt ctacccgtac atcgaccatg ccggcacgcg cctgatcggc
     2221 gtcgaggccg ccgggcaggg ccttgccacc ggcaagcacg ccgcgtcgct cagcgccggc
     2281 tcgccgggcg tgctgcacgg caaccgcacc tatctgctgc agg
//
LOCUS       DATMJB010000464         2716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_303024, whole genome shotgun sequence.
ACCESSION   DATMJB010000464 DATMJB010000000
VERSION     DATMJB010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303024"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VNU48_09930"
     CDS             <1..323
                     /locus_tag="VNU48_09930"
                     /inference="COORDINATES: protein motif:HMM:NF020467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1839 family protein"
                     /protein_id="HWI11637.1"
                     /translation="TTRQFGANMSLLGAHLAWLDRHAGGDLGGDLGGDLGNAVVLCER
                     MATGAKTFQFLLARALARKRFDKLDPALDAIARDYDAAMAAITSALRVPLLEKAPALI
                     PASS"
     gene            332..>2716
                     /locus_tag="VNU48_09935"
     CDS             332..>2716
                     /locus_tag="VNU48_09935"
                     /inference="COORDINATES: protein motif:HMM:NF036482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HWI11638.1"
                     /translation="MARVATVSGDTTIPLTANWSVALARPDEALTPDAAQSCRWVPAR
                     VPGTVGRDLAVPEGESLHDYDVWYRCHFDADGPQSLYLRGLAGIAEVFLDDRLVVSSA
                     SMFIENCVDFDGRGRHALAIRFKALARVLADVSGPRARWRPMMIVPSTLRHVRISPLG
                     HMPGWAPQIPLIGPWKPVEIVARRAPFRVACLRLVPSLLGAAGHLAIDIRLDRPVDAA
                     AELVCATARLALDRTGPDRFSGVLVIDGIAPWWPHTHGEPALHRAHLKIGDTTLDLGR
                     IGFRKLAVDRGADGRGFALRVNDQPIFCRGASWMPPDPLAPGSADPRAYIAKARDAGM
                     NMLRISGTATPESEAFHHACDEAGVLVWHDLPFANFDYPTTETSFAALVETEVRQLLD
                     GLQASPSFAVVCGGSEIAQQATMLGLMPAQAHNSFFEETAPALVAELAPQAVYVPHTP
                     WGGSLPFVTDEGVTHYFGVGAYRRPMQDVRHSQVRFATECLAFANVPSVRRLRDAGLS
                     DPADPRWKAGVPRDLGAEWDFEDVRDHYVGTLYSVDAEALRTSDPQRYLALGRAAPAE
                     LAQSVFAEWRRAGSSCGGGLLWFLNDLAPGAGWGALDDRGDPKCIWYALRRAFQPIHL
                     GITDEGLNGLALHLGNETQSPRTLRLSLMCFGDGPHPSAKAERNITLGPRESVSLSSN
                     ALLGRFFDITYAYRFGPLAHDATIARLLDPETGAIIAEATHVLQGRAATPRDIGLTAE
                     VTPSGAGFALAVRAERFARFVTIDDEQFMAADQGFCLVPGERRLVPLSSASAVAK"
BASE COUNT          401 a          933 c          865 g          517 t
ORIGIN      
        1 ataccacccg gcagtttggc gccaacatgt cgcttctggg tgcgcacctc gcctggctag
       61 atcggcacgc gggcggggac ttgggtgggg acttgggcgg ggacttgggc aacgccgttg
      121 tcctttgcga gagaatggca accggcgcca agacgttcca gtttctcctg gcgcgagcgc
      181 tggcccgcaa gcgtttcgac aagctcgatc ctgcgcttga cgcgattgcc cgcgactacg
      241 acgccgccat ggcggccatc acctctgcac tgcgcgttcc gcttctggag aaggctccag
      301 cgctcatccc ggccagttcg tagaggctcg gatggcgcgg gtcgcgaccg tcagtggcga
      361 tacgactatt ccgcttactg cgaactggag cgtggcactg gcgcgaccgg acgaggcgct
      421 gacgcccgat gctgcgcaat cgtgccgctg ggtcccggct cgcgttcccg gcacggtcgg
      481 ccgcgatctc gctgtccccg aaggcgaaag tctccacgac tacgacgtct ggtaccgctg
      541 ccactttgac gccgacgggc cgcagagcct gtacctgcgc ggcctcgccg gaattgccga
      601 ggtcttcctc gacgaccgtc tcgttgtgtc ctccgcgtca atgttcatcg agaattgtgt
      661 cgatttcgac ggccggggcc gacatgcgct cgccatccgc ttcaaggcgc tggcgcgtgt
      721 cctcgccgat gtcagtggtc cgcgcgcccg ttggcggccg atgatgatcg tgccgtccac
      781 cctgcgccac gtgcgcatct cgccgctcgg tcacatgccg ggctgggctc cgcagatccc
      841 gctcatcggt ccatggaagc cggtcgagat cgtggcgcgt cgtgcgccct tccgggtcgc
      901 gtgcctccgg ctggttccgt cgctgctcgg cgcagccggt cacctggcga tcgacattcg
      961 cctcgaccgg ccagtcgatg ctgctgcaga gctcgtatgt gcgaccgcac ggcttgcgct
     1021 cgatcgaacc gggcctgatc gctttagtgg cgtgctcgtg atcgacggca tcgccccttg
     1081 gtggccgcac acccatggcg aaccggcctt gcacagggct catttgaaga tcggcgatac
     1141 cacgctcgac ctgggccgca tcgggtttcg taagctcgcc gtggaccgtg gagctgacgg
     1201 ccgcggcttt gccctgcgcg tcaatgacca gccgatcttc tgccgcggcg ccagctggat
     1261 gccgccggac ccgctggcgc caggcagcgc cgatccgcgc gcgtacatcg cgaaagcacg
     1321 cgatgccggc atgaacatgc tgcgtatctc aggcaccgcc acacccgaat cggaggcttt
     1381 tcatcacgcc tgcgatgagg caggcgtgct tgtctggcac gatctgccgt tcgctaattt
     1441 cgactatccg accactgaga ccagttttgc cgcgcttgtc gaaacggaag tgcggcaact
     1501 gctcgacggt ctgcaggcct caccctcgtt cgcagttgtc tgcggcggca gcgagatcgc
     1561 gcaacaggcg accatgctgg ggctcatgcc ggcgcaggcg cacaacagct tcttcgagga
     1621 gaccgcgccg gcgctcgtcg ccgagctggc accgcaggcg gtgtatgtcc cccatacacc
     1681 atggggggga tcgctgccgt ttgtcaccga cgaaggcgtt acccactatt tcggtgtcgg
     1741 cgcctatcgg cggccaatgc aggacgtccg gcattcgcag gtgcgcttcg ccaccgaatg
     1801 cctcgccttc gccaacgtgc cttcggtccg gcggctgcgt gatgccggtc tctcggatcc
     1861 ggcggacccc cgatggaagg caggcgtgcc ccgcgacctc ggggcggaat gggacttcga
     1921 ggatgtgcgc gatcactatg tcggcacgct ttacagcgtc gacgcggaag cgctgcgcac
     1981 gagcgatccg caacgttatc tcgcgctcgg acgcgctgct cccgccgagc tggcgcaatc
     2041 cgtgttcgcc gaatggcggc gcgccggttc atcctgcggt ggcggcctcc tctggttcct
     2101 caacgatctc gcgccgggcg ccggatgggg agcgcttgat gaccggggcg atccgaaatg
     2161 catctggtac gcgctgcgcc gtgccttcca gccaatccat cttggcatca cagacgaagg
     2221 cctgaacggc ctcgcactgc atcttggtaa cgagacccaa tcgccgcgca ccttgcggct
     2281 gtcgctgatg tgtttcggcg atggcccgca tccatctgcg aaagccgagc ggaacatcac
     2341 gctcggcccg cgcgaatctg tcagcctatc gagcaatgcc ctgctcggtc gcttctttga
     2401 catcacctat gcctatcgct ttggtccgct cgcgcacgac gccaccattg ctcgcctgct
     2461 cgatccggag accggcgcca tcattgccga agcgacccac gtgctgcagg ggcgtgccgc
     2521 gacgccgcgt gacatcggcc ttaccgccga ggtcacaccg agcggcgcag ggtttgcgct
     2581 tgcagtccgt gcggaacgtt tcgcccgctt cgtcactatc gacgacgagc aattcatggc
     2641 cgccgatcag ggcttctgcc tggttcccgg cgagcgccgg ctcgttcccc tgtcatcggc
     2701 cagcgccgtt gcgaag
//
LOCUS       DATMJB010000465         2357 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_310754, whole genome shotgun sequence.
ACCESSION   DATMJB010000465 DATMJB010000000
VERSION     DATMJB010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310754"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /gene="ispH"
                     /locus_tag="VNU48_09940"
                     /gene_synonym="lytB"
     CDS             complement(<1..982)
                     /gene="ispH"
                     /locus_tag="VNU48_09940"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWI11639.1"
                     /translation="MHADLQEIVLAEPRGFCAGVDRAIEIVERALKKFGAPIYVRHEI
                     VHNTYVVNDLKAKGAIFIEELADVPPGATLVFSAHGVSQQVRREAAQRGFSVFDATCP
                     LVTKVHVEVAKLHKLGYDFIMIGHKGHPEVEGTMGQLTEGIYLVEDVADVATVQVAQA
                     AKLAVVTQTTLSVDDAAEILAAVKRRFPGVREPKQQDICYATQNRQDAVKVLAPTVDV
                     VIVVGSPTSSNSNRLRELAQRLGTAGHMVDSADDLKPEWFEGKRRVGLTAGASAPELL
                     VQQVIERLRALGAVSVRSLAGAVETVHFPLPKGLGDKAMGLPPGTHGPLSA"
     gene            complement(982..1428)
                     /locus_tag="VNU48_09945"
     CDS             complement(982..1428)
                     /locus_tag="VNU48_09945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWI11640.1"
                     /translation="MSQKVQPGSFLTLHYRLSGPDGTDLINTFDDKPATLSLGTGELA
                     PAMEARLLGLPEGTRRSFELAAGEAFGERNPELLQRVKRSLLTEFGDPDERYAVGDVV
                     RFPTPDGAGAYAGVLREVDGDVLVFDFNHPLAGRPVTFEVQLIGVL"
     gene            1527..2201
                     /gene="radC"
                     /locus_tag="VNU48_09950"
     CDS             1527..2201
                     /gene="radC"
                     /locus_tag="VNU48_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HWI11641.1"
                     /translation="MVLKDIPADARPREKLLASGAAALADAELIALLLRTGLKGVSVL
                     QLAQQMLDRFGGIQGLLHAQVDDLKRIKGLGPAKRAEIAAVIELARRALMQQLAERPV
                     FDAPAKVKQYLQLQLGGHVFEVFAVMFLDAQNRLLKLEEMFRGTLTQTSVYPREVVRR
                     ALELHAGAVILAHNHPSGAAEPSRADEYLTQTLKSALQLVDVRVLDHLVVGRGEVVSF
                     AERGLL"
BASE COUNT          354 a          834 c          816 g          353 t
ORIGIN      
        1 cggcgctgag cggcccgtgc gtcccgggcg gcaagcccat cgccttgtcg cccagcccct
       61 tcggcagcgg gaagtgcacg gtctcaaccg cgccggccag gctgcgcacc gacaccgcgc
      121 cgagcgcacg caaccgctcg atcacctgct ggacgaggag ttccggcgcc gaggcacccg
      181 ccgtcaatcc aacgcggcgc ttgccctcga accactcggg cttcaggtca tcggccgaat
      241 cgaccatgtg gccggccgtg ccgaggcgct gcgccagctc gcgcagccgg ttgctgttcg
      301 agctggtcgg gctgccgacg acgatcacca cgtccaccgt cggcgccagc accttgaccg
      361 cgtcctggcg gttctgcgtc gcatagcaga tgtcctgctg cttgggctcg cgcacgccgg
      421 ggaagcgccg cttcaccgcc gcgaggatct cggccgcgtc gtcgaccgac agcgtggtct
      481 gcgtcacgac cgcgagcttc gccgcctgcg cgacctgcac cgtcgcgaca tcggccacgt
      541 cctcgacgag gtagatgccc tcggtgagct gccccatcgt gccctcgacc tcggggtggc
      601 ccttgtggcc gatcatgatg aagtcgtagc cgagcttgtg cagtttcgcg acctcgacat
      661 gcaccttggt gaccagcggg caggtcgcgt cgaacacgct gaagccgcgc tgcgccgcct
      721 cgcgccgcac ctgctgcgac acgccgtgcg cgctgaacac cagggtcgcg ccgggcggca
      781 cgtcggccag ctcctcgatg aagatcgcgc ccttggcctt caggtcgttg accacgtagg
      841 tgttgtgcac gatctcgtgg cgcacgtaga tcggcgcacc gaacttcttc agcgcgcgct
      901 cgacgatctc gatcgcgcgg tccacgccgg cgcagaagcc gcgcggctcg gccagcacga
      961 tctcttgcag gtcggcatgc atcacagcac cccgatcagc tgcacttcga aggtcaccgg
     1021 ccggccggcg agcgggtggt tgaagtcgaa caccagcacg tcgccatcga cctcgcgcag
     1081 cacgccggcg taggcgccgg cgccgtcggg cgtcgggaac cggaccacgt cgccgacggc
     1141 gtagcgctcg tcagggtcgc cgaactcggt cagcagcgag cgcttcacgc gctgcagcag
     1201 ctcggggttg cgctcgccga aagcttcgcc ggcggcaagt tcgaacgagc gccgcgtgcc
     1261 ctccggcagc ccgagcaggc gtgcctccat cgcgggcgcg agttcgccgg tgcccagcga
     1321 cagggtcgcc ggcttgtcgt cgaaggtgtt gatcaggtcg gtgccgtcgg gccccgacag
     1381 gcggtagtgc agggtcagga acgaccccgg ttggactttt tggctcaagc gaaatcctga
     1441 caaggctggg tgtcgaccga tttcagccga tagactgggg cgattttacg aggcggctgc
     1501 aaagcccctg aatcgacaac cgcatcgtgg tgctcaagga catccccgcc gacgcccggc
     1561 cgcgcgagaa gctgctcgcc agcggcgcgg ctgcgctggc cgatgccgag ctgatcgcgc
     1621 tgctgctgcg caccgggctg aagggcgtgt cggtgctgca gctcgcgcag cagatgctcg
     1681 accgcttcgg cggcatccag ggcctgctgc atgcgcaggt ggacgacctg aagcgaatca
     1741 agggcctggg gccggccaag cgcgccgaga tcgccgcggt gatcgaactc gcgcggcgcg
     1801 cgctgatgca gcagctcgcc gaacgcccgg tgttcgatgc gcccgccaag gtcaagcagt
     1861 acctgcagct gcagctcggc ggccatgtgt tcgaggtgtt cgcggtgatg ttcctcgatg
     1921 cgcagaaccg gctgctcaag ctcgaggaga tgttccgcgg caccttgacc cagaccagcg
     1981 tctacccgcg cgaggtcgtg cggcgcgcgc tcgaactgca cgccggcgcg gtgatcctgg
     2041 cccacaacca cccgtcgggc gcggccgagc cgtcgcgcgc cgacgagtac ctgacgcaga
     2101 cgctgaagag cgcgctgcaa ctggtggacg tgcgcgtgct cgaccacctg gtcgtcggcc
     2161 gcggcgaggt ggtgtcgttc gccgagcgtg gcctgctgtg acgaaggcgg cgaccaaacc
     2221 gcgcacgctg gccgagctcg gcgcgctcaa gcaggccttg cagcatgcgc agcgcgaggc
     2281 ggcgcgcagc gccgaacgcg agcgcgagcg cctcgcccgc gagcagcgcg agcgcgacct
     2341 gttcgcgctg acggtcg
//
LOCUS       DATMJB010000466         3096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_530951, whole genome shotgun sequence.
ACCESSION   DATMJB010000466 DATMJB010000000
VERSION     DATMJB010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530951"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..360
                     /locus_tag="VNU48_09955"
     CDS             28..360
                     /locus_tag="VNU48_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HWI11642.1"
                     /translation="MTRSDLVAQLAERFGQLTHRDTEFAVKTILDAMSAALAKGHRIE
                     IRGFGSFSINRRPPRVGRNPRSGEQVVIPEKLVPHFKPGKALREAVDAHVPIGSAALA
                     HALAGDDD"
     gene            423..728
                     /locus_tag="VNU48_09960"
     CDS             423..728
                     /locus_tag="VNU48_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HWI11643.1"
                     /translation="MRLLVWLFRAAIFFTLFAFALNNQQEAGVRWFFGQEWRAPMVFI
                     VLAAFALGCAFGVFAMVPSWWRHRRVARKHAPVIEARPDVPMTSPAAEITHPPRDGL"
     gene            718..1869
                     /gene="lapB"
                     /locus_tag="VNU48_09965"
     CDS             718..1869
                     /gene="lapB"
                     /locus_tag="VNU48_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HWI11644.1"
                     /translation="MDFDLQWLLLGLPLAFAFGWLASRFDLRQWKREQKDSPKAYYKG
                     LNLLLNEQHDKAIDAFIEAVQQDPDTSDLHFALGNLFRRRGEFERAVRVHEHLLNRAD
                     LPRSERDRAQDALAQDFMKAGLFDRAESAYLALEGTAFDTEARLALLSLHERSRDWRA
                     AVEVAGKLEHRGTGSFASRISHYWCEIALEAEAKQQSDEADSALKRAREAAPQAARPL
                     VLAGQRAARRGDHAEVLALWGVLMVAHPASFTLVAHEYAQSALASGGGAQALARLREL
                     YARAPTMDLLDALALLDPEPARQRARLLAQLRQHPTLSVAKALITQQEVAHFDAGETQ
                     ALGDAIGRAAKPLHRYRCAACGFEAQHYFWQCPGCLNWDSYPPQRLEDL"
     gene            1869..2804
                     /gene="rfaE1"
                     /locus_tag="VNU48_09970"
     CDS             1869..2804
                     /gene="rfaE1"
                     /locus_tag="VNU48_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HWI11645.1"
                     /translation="MATHPSREKVEHSRVLVVGDAMLDRYWFGAVDRISPEAPVPVVR
                     VNREEERLGGAANVALNVKTLGAQATLLTVVGDDEPARKLRHLLEQLGVTALLGSDPK
                     LYTIVKLRVIGRSQQLIRIDFENQPEHEVLVGMLADFERVLPEHDAVLFSDYGKGGLT
                     HIPRMIDLARQLGKPVLIDPKGSDYGRYAGATVITPNRAELAQVIGSWSSEAQLHERV
                     QALRTAHRLDALLLTRSEDGMSLFDAQGHVQVAAEAREVFDVTGAGDTVIATLAAMLG
                     AGLSLREAMPIANRAGGIVVGKFGTASVGYEELFA"
     gene            2801..>3096
                     /locus_tag="VNU48_09975"
     CDS             2801..>3096
                     /locus_tag="VNU48_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWI11646.1"
                     /translation="MKVVVTGAAGMIGSNLVHGLNAIGIDDVIAVDDLTDGPKYRNLL
                     GAQLSDYFDKREFYARFARGEFGHVDALFHEGACSDTMEHDGRFMLDTNYRCSK"
BASE COUNT          467 a         1118 c         1051 g          460 t
ORIGIN      
        1 ccgcatcccc tcgggcgagg ggcgccaatg acccgctccg atctcgttgc gcaattggcc
       61 gaacgtttcg gccagctgac gcatcgcgac acggagttcg cggtcaagac gatcctcgat
      121 gcgatgtccg cggcgcttgc caaggggcac cgcatcgaga tccgcggatt cggcagcttc
      181 tcgatcaacc gccggccgcc gcgcgtgggc cgcaacccgc gctccggcga gcaggtggtg
      241 attcccgaga agctcgtccc gcatttcaag ccgggcaagg cgctgcgcga ggccgtcgac
      301 gcacacgtgc ccatcggctc ggcggcgctg gcgcacgccc tcgccggcga cgacgactga
      361 acgcagcttc gctcgacccg gccggtagaa tcggccgcct gttccgattg cgttcaccgc
      421 cgatgcgatt gcttgtctgg ctcttccggg ccgccatctt cttcacgctc ttcgccttcg
      481 cgctgaacaa ccagcaggag gccggcgtgc gctggttctt cggtcaggaa tggcgcgcgc
      541 cgatggtctt catcgtgctc gccgcgttcg cgctcggctg cgcattcggc gtgttcgcga
      601 tggtgccgag ctggtggcgg caccgccgtg tggcgcgcaa gcatgccccg gtgatcgagg
      661 cccggcccga cgtgccgatg acgagcccgg ccgcagagat cacgcacccg cctcgcgatg
      721 gactttgacc tgcaatggct gctgctcggt ctgccgctgg cgttcgcgtt cggctggctc
      781 gcgtcgcgct tcgacctgcg ccagtggaag cgtgagcaga aggactcccc gaaggcctac
      841 tacaagggcc tgaacctgct gctcaacgaa cagcacgaca aggccatcga cgccttcatc
      901 gaggccgtgc agcaagaccc cgacacctcg gacctgcact tcgcgctggg caacctgttc
      961 cggcgccgtg gcgagttcga gcgcgcggtg cgcgtgcacg agcacctgct caatcgtgcc
     1021 gatttgccgc gcagcgagcg cgaccgcgcg caggacgcgt tggcccagga cttcatgaag
     1081 gccggcctgt tcgaccgcgc cgaaagcgcc tacctcgcgc tcgaaggcac ggccttcgac
     1141 accgaagcgc gcctggcgct gctgtcgctg cacgagcgct cgcgcgactg gcgcgccgcg
     1201 gtcgaggtcg cgggcaagct cgagcaccgc ggcaccggct cgttcgcgtc gcgcatctcg
     1261 cactactggt gcgagatcgc gctcgaggcc gaggcgaagc agcagtccga cgaagccgac
     1321 agcgcgctga agcgcgcccg cgaggccgcc ccgcaggcgg cgcgcccgct ggtgctggcc
     1381 ggtcagcgcg cggcgcgccg cggcgaccac gccgaggtgc tggcactgtg gggcgtgctg
     1441 atggtcgcgc acccggcctc gttcaccctc gtcgcacacg agtacgcgca gagtgcgctc
     1501 gccagcggcg gcggcgcgca ggcgctcgcg cggctgcgcg agctgtacgc gcgagcgccg
     1561 acgatggacc tgctcgatgc gctggcgctg ctcgatcccg agccggcgcg ccagcgcgcg
     1621 cggctgctcg cgcagctgcg tcagcacccg acgctgtcgg tcgcgaaggc cctgatcacg
     1681 cagcaggagg tcgcgcactt cgatgccggc gagacccagg cgctcggcga cgcgatcggc
     1741 cgcgccgcca agccgctgca ccgctaccgt tgcgcggcct gcggcttcga ggcgcagcac
     1801 tatttctggc aatgtcccgg ctgcctgaac tgggacagct acccgccgca gcggctggag
     1861 gacttgtaat ggccactcac ccctcgcgcg agaaggtcga gcactcgcgc gtgctcgtcg
     1921 tcggcgacgc gatgctcgac cgctactggt tcggcgccgt cgaccgcatc tcgcccgagg
     1981 cgccggtgcc cgtggtgcgc gtcaaccgcg aggaggagcg tctcggcggc gccgccaacg
     2041 tcgcactgaa cgtcaagacg ctgggcgcgc aggccacgct gctgaccgtc gtcggcgacg
     2101 acgagccggc gcgcaagctg cgccacctgc tcgagcagct tggcgtgacc gcgctgctcg
     2161 gcagcgaccc gaagctctac acaatcgtca agctgcgcgt catcggccga tcccagcagc
     2221 tgatccgcat cgacttcgag aaccagcccg agcacgaggt gctcgtcggc atgctggccg
     2281 acttcgagcg cgtgctgccc gagcacgacg cggtgctgtt ctccgactac ggcaagggcg
     2341 gcctgaccca catcccgcgc atgatcgacc tcgcgcgaca gctcggcaaa ccggtgctga
     2401 tcgaccccaa gggcagcgac tacggccgct acgccggcgc caccgtgatc accccgaacc
     2461 gcgccgagct cgcccaggtc atcggcagct ggagcagcga ggcgcagctg cacgagcgtg
     2521 tgcaggcgct gcgcacggcg caccggctcg acgcgctgct gctgacccga tccgaggacg
     2581 gcatgtcgct gttcgacgca cagggtcacg tgcaggtggc ggccgaggcg cgcgaggtct
     2641 tcgacgtgac cggtgccggc gacaccgtga tcgccacgct cgcggcgatg ctcggcgcgg
     2701 gcctgagcct gcgcgaggcg atgcccatcg cgaaccgcgc cggcggcatc gtcgtcggca
     2761 agttcggcac cgccagcgtc ggctacgagg agttgttcgc atgaaggtcg tcgtcaccgg
     2821 cgctgccggc atgatcggct cgaatctcgt gcatggcctg aacgcgatcg gcatcgacga
     2881 cgtgatcgcc gtcgacgatc tgaccgacgg cccgaagtac cgcaacctgc tgggcgcgca
     2941 gctcagcgac tacttcgaca agcgcgagtt ctacgcacgc ttcgcccgag gcgagttcgg
     3001 gcacgtcgat gccctgttcc acgaaggcgc ctgttccgac acgatggagc acgacgggcg
     3061 cttcatgctc gacaccaact accgctgctc caagga
//
LOCUS       DATMJB010000467         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_599809, whole genome shotgun sequence.
ACCESSION   DATMJB010000467 DATMJB010000000
VERSION     DATMJB010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599809"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VNU48_09980"
     CDS             <1..1229
                     /locus_tag="VNU48_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HWI11647.1"
                     /translation="DRIMFPIRSVQGDVIGFGGRVLDRGEPKYLNSPETPVFSKGREL
                     YGLFEGRAALRQRGYALVVEGYMDVVALAQSGFGNAVATLGTACTPEHLQKLFRFTDS
                     IVFSFDGDAAGRRAARRALETSLPHASDTRTIRFLFLPTEHDPDSYVREFGPAAFEEC
                     VAQATPLSRQLIEAAADGCDLTTAEGRAHMLANAKPLWTALPEGTLKRQLLTEIAGAA
                     RDEVDQLLRQWGHAPARPAARDAAPARVPTRRAGALSKGTANLLDRAIWLLLQRSELW
                     ATLDGESHDFLAGQAAPYDLFFGALERSIHEHGALSFAALLDELRQQAADAPHAANVI
                     KRIAEFHASDANVDVARELALVLDGLRLRAVRDELDLMVEGGLTSPDAHQRFKLLNGE
                     LARLKNLLASQQNASN"
     gene            complement(1550..1690)
                     /locus_tag="VNU48_09985"
     CDS             complement(1550..1690)
                     /locus_tag="VNU48_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11648.1"
                     /translation="MASGFGALKGFAGALLAFFGSAVLLPETGAAFAAGALFFAVMQI
                     LG"
     gene            1689..>2742
                     /locus_tag="VNU48_09990"
     CDS             1689..>2742
                     /locus_tag="VNU48_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HWI11649.1"
                     /translation="MNKKPAKPAAKLVELPKGKAAGRKPKIDEKAKKPEDNDVDLSDL
                     EADLEGEPEVETADETKVKAKPLRMKVSRAKERALMREFGLEETALTEEEATKRRQEL
                     KTLIKMGKTRGFLTHQEINDHLPEKLVNDEILEAIVSMLNDMGIAVYEQAPDAATLLI
                     AGGGTTTATEEEAEEAAEAALSTVDSEFGRTTDPVRMYMREMGTVELLTREGEIEIAK
                     RIEGGLQAMMLAISASPTTIAEILVYADRIAAGEMQISEVVDGFVSEDEADDYVAEED
                     VDFFDEEDDDDGQGGSKALTKKLEELKTAALVKFDALRTHFDKMRKAYEKEGYKSASY
                     NKAQHSISDDLMTIRFT"
BASE COUNT          531 a          946 c          909 g          356 t
ORIGIN      
        1 gcgatcgcat catgttcccg atccgctcgg tgcagggcga cgtgatcggc ttcggcggcc
       61 gcgtgctgga ccgcggtgaa ccgaagtacc tgaactcgcc cgagacaccg gtgttcagca
      121 agggccgcga actctacggc ctgttcgaag gccgggccgc gctgcgtcaa cggggctacg
      181 cgctggtggt cgagggctac atggacgtgg tcgcgctggc gcagtcgggc ttcggcaatg
      241 ccgtggccac gctcggcacc gcctgcacgc ccgagcacct gcagaaactc ttccggttca
      301 ccgactcgat cgtgttcagc ttcgacggcg acgccgccgg gcgacgcgcg gcgcggcgcg
      361 cgctcgaaac cagtctgccg cacgcgagcg acacgcgcac gatccgcttc ctgttcctgc
      421 cgaccgagca cgaccctgac tcctatgtgc gcgaattcgg cccggcggcg ttcgaggaat
      481 gcgtcgcgca ggcgacgccg ctgtcgcgcc agctgatcga ggcggcggcc gacggctgcg
      541 acctgacgac cgccgagggc cgcgctcaca tgctggccaa cgcgaagccg ctgtggaccg
      601 ccctgcccga gggcacgctg aagcggcagt tgctgaccga aatcgccgga gccgcccgcg
      661 acgaggtcga tcagttgctg cgccaatggg gccacgcgcc ggcgcgcccc gccgcgcgcg
      721 acgccgcacc agccagggtg ccgacgcgcc gtgccggcgc gctcagcaag ggcacggcca
      781 atctgctcga ccgggcgatc tggctgctgc tgcagcgcag tgaactgtgg gcaacgctcg
      841 acggcgagtc gcacgacttc ctggccgggc aggctgcacc ctacgacctg ttcttcggcg
      901 ccctcgagcg cagcatccac gagcacggcg cgctgtcgtt cgccgcgctg ctggacgagt
      961 tgcgtcagca ggccgccgac gcgccgcacg cggccaacgt gatcaagcgg atcgccgagt
     1021 tccatgcctc cgacgcgaac gtcgacgtgg cccgggagct ggcgctggta ctcgacggcc
     1081 tgcggctgcg ggcggtccgc gacgagcttg atctgatggt cgaaggcggc ctcacttcgc
     1141 ccgacgcgca ccagcgattc aagctgctca acggcgagct cgccagactg aagaatctgc
     1201 tggccagcca gcaaaacgcc agcaactgac ctggcggaat gcgtgttccg tgggtataat
     1261 cctcggttga cgttagcgcg gcaagagtga cagcagcacc tccgggcccc ggccaccctc
     1321 tgacccaggg tccccatgcg gggttacagg ccacccattc ccgcaaggac tgcacgccca
     1381 aatgatcacc cgagcttgcc cgctcgatgt cggcccggtc gatgcgacgg cgggccgagc
     1441 gacgattcga tcgaccatcc cacgggcctt tccgatcggg tgcctctgca cttgaattgc
     1501 gccatgtcgc gcgcagttca gggacagggc ccgcatcgcc agcccgccat catccgagga
     1561 tctgcatgac cgcgaagaag agcgccccgg ccgcgaaggc cgcccctgtc tcaggcaaca
     1621 agacggcgga accgaagaac gccaagagcg cccccgcgaa gcctttgagg gcgccgaagc
     1681 ccgatgccat gaacaagaag cccgccaagc cggcggccaa gctcgtcgaa ctgcccaagg
     1741 gcaaggcggc ggggcgcaag cccaagatcg acgagaaggc gaagaagccg gaagacaacg
     1801 acgtcgacct gtccgacctc gaggccgacc tcgaaggcga gccggaggtc gagaccgccg
     1861 acgagaccaa ggtcaaggcc aagccgctgc gcatgaaggt gtcgcgtgcc aaggagcgcg
     1921 cgctgatgcg cgagttcggc ctcgaagaga ccgcgctgac cgaagaggaa gcgaccaagc
     1981 gccgtcagga gctgaagacg ctcatcaaga tgggcaagac gcgcggcttc ctgacgcacc
     2041 aggaaatcaa cgaccaccta cccgaaaagc tggtcaacga cgagatcctc gaggccatcg
     2101 tctcgatgct caacgacatg ggcatcgcgg tctacgagca ggcgcccgac gccgccacgc
     2161 tgctgatcgc cggcggcggc acaaccaccg cgaccgagga agaagccgag gaagccgccg
     2221 aagcggcgct gtccaccgtc gactccgaat tcggccgcac gaccgacccg gtgcgcatgt
     2281 acatgcgcga gatgggcacg gtggagctgc tcacccgcga gggcgagatc gagatcgcca
     2341 agcgcatcga aggcggcctg caggccatga tgctggccat cagcgcctcg cccacgacca
     2401 tcgccgagat cctggtctac gccgaccgca tcgcggccgg tgagatgcag atctccgagg
     2461 tggtcgacgg cttcgtctcc gaagacgagg ccgacgacta cgtggccgag gaagacgtcg
     2521 acttcttcga cgaggaagac gacgacgacg gccagggcgg cagcaaggcg ctgaccaaga
     2581 agctcgagga gctgaagacc gcggcgctgg tgaagttcga cgccctgcgc acccacttcg
     2641 acaagatgcg caaggcctac gagaaggaag gctacaagtc cgcctcgtac aacaaggcgc
     2701 agcacagcat cagcgacgac ctgatgacga tccgcttcac gg
//
LOCUS       DATMJB010000468         3005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_72523, whole genome shotgun sequence.
ACCESSION   DATMJB010000468 DATMJB010000000
VERSION     DATMJB010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72523"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..989)
                     /locus_tag="VNU48_09995"
     CDS             complement(3..989)
                     /locus_tag="VNU48_09995"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWI11650.1"
                     /translation="MKRNALLIGAFVLAALAMSVVGVLWLSGNNLFKRQQEAMIFYKG
                     NVSGLYVGAPVTFRGVATGQVEAINVEVDSKTLAARIAVRVSLQQNSLRFIDDNPDTA
                     FNLPHLVQRGLRARLVAQSFVTGQKSIDLDFLPGTPAVLVAGGTRPEIPALGDRFGAL
                     IDQVAELPLRDTVQEIRDTVKELRGTLVSVQHTLDGTQVVLTGVSKEIAGAGTASRET
                     LKAATDAIKSVQANSAATLDSITKLSDTTRQTVLAAQPDLQRTLAGTREAAESARLAM
                     DRVAQMTAPDAALRSDLDAAIRDLSQAARGLRDWSELLEEKPNAVIFGNKRE"
     gene            complement(1027..1803)
                     /locus_tag="VNU48_10000"
     CDS             complement(1027..1803)
                     /locus_tag="VNU48_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI11651.1"
                     /translation="MTLLAVDDLEMRFGERLIQTGVSFKVEKGSIFAIMGGSGCGKST
                     LLKHLIGLLRPAKGSVTYDSVDYWRADEAGRARLRTGFGMLFQSAALWSSMTILENVC
                     LPLERQGRLDKAQREARAREVLTWVDLGQFADFYPADLSGGMKKRAGLARAIGGEPPL
                     LFLDEPSAGLDPISSKKLDNLVLDIRARTGCAVVFVSHELPSLFAIADDGIFLDADSK
                     TPIAHGSPSMLRDTATHPTVRAFLAREEPPAPAARSGASP"
     gene            complement(1808..2572)
                     /locus_tag="VNU48_10005"
     CDS             complement(1808..2572)
                     /locus_tag="VNU48_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI11652.1"
                     /translation="MQIASLLPIRPVVFLGQVLISAVEAVLGRWQFRLKDLGRITADC
                     SARAVPIVTVVNVLVGAILAFVGAVQLVKFGAGIYVADLVGIAVAREMAAIITAVVMA
                     GRTGAAFAAELATMQANEEVDALEVLGLSAVDFLVLPRVMALLLMMPLLYVYGCVTGL
                     LGGMLVAAGMLDISPAAYLDRTLVALDWSHLGLGFSKSIVFGALIGMVGCYFGLYAQR
                     NAEGVGVATTNAVVVSIVGIIVLDAVFAVCANALGI"
     gene            2691..>3005
                     /gene="argB"
                     /locus_tag="VNU48_10010"
     CDS             2691..>3005
                     /gene="argB"
                     /locus_tag="VNU48_10010"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWI11653.1"
                     /translation="MTTDAAAAPAADAPDLSHISPRDKAEILAQALPYIRKFHGKTIV
                     IKYGGNAMTDPGLQQDFAEDVVLLKLVGMNPVVVHGGGPQIDEALSRLGKKGTFIQGM
                     RVT"
BASE COUNT          474 a         1033 c         1044 g          454 t
ORIGIN      
        1 gttcattcgc gtttgttccc gaagatgacg gcgttgggct tttcttccag cagttcgctc
       61 cagtcgcgca ggccgcgcgc ggcctgcgac aggtcgcgta tcgcggcgtc gaggtcgctg
      121 cgcagtgcag cgtcgggcgc ggtcatctgg gccacgcggt ccatcgccag gcgcgccgac
      181 tcggctgcct cgcgggtgcc ggcgagggtg cgctgcaggt cgggctgcgc tgccagcacg
      241 gtctggcggg tggtgtcgga aagcttggtg atcgagtcga gtgtcgcggc cgagttggcc
      301 tgtaccgact tgatcgcatc ggtggcggcc ttcagcgtct cgcgcgaggc cgtgccggcg
      361 ccggcgatct ccttgctgac gccggtcagc acgacctgcg tgccgtcgag cgtgtgctgc
      421 accgagacca gcgtgccccg cagctctttc acggtgtcgc ggatctcctg cacggtgtcg
      481 cgcagcggca gctcggcgac ctggtcgatc agcgcgccga agcggtcacc cagcgccggg
      541 atctccggcc gcgtgccgcc ggcgacgagc acggcaggcg tgcccggaag gaagtcgagg
      601 tcgatcgact tctgcccggt gacgaagctt tgcgccacca ggcgggcgcg caggccgcgc
      661 tgcaccagat gcggcaggtt gaacgccgtg tcggggttgt catcgatgaa gcgcaacgaa
      721 ttctgctgca gcgacacgcg cacggcgatg cgagccgcca gtgtcttgct gtcgacttcg
      781 acgttgatgg cttcgacctg accggtcgcg acgccgcgga aggtgacggg cgcgcccacg
      841 tacagacccg agacgttgcc cttgtagaag atcatcgcct cttgctgccg cttgaacagg
      901 ttgttgccgc tgagccagag cacgccgacg acgctcatcg cgagcgccgc gagcacgaag
      961 gcaccgatca gcagcgcgtt gcgtttcatg gcgtggccgg ggtcgaggag ggcatggttg
     1021 cggatgctac gggcttgccc cgctccgtgc agcgggcgct ggcggttcct cgcgcgccag
     1081 gaaggcgcgc accgtcgggt gcgtggccgt gtcgcgcagc atgctcgggc tgccgtgggc
     1141 gatcggggtc ttgctgtcgg cgtcgaggaa gatgccgtcg tcggcgatcg cgaacaggct
     1201 cggcaattcg tggctgacga ataccaccgc gcagccggtg cgcgcgcgga tgtcgagcac
     1261 caggttgtcg agcttcttcg agctgatcgg gtccagcccg gccgacggtt cgtccaggaa
     1321 cagcagcggc ggctcgccgc cgatcgcgcg cgccagcccg gcgcgcttct tcatgccgcc
     1381 ggacagatcg gcggggtaga aatcggcgaa ctggccgagg tcgacccacg tcagcacctc
     1441 gcgcgcgcgc gcctcgcgct gcgccttgtc gagtcggccc tgccgctcca gcggcaggca
     1501 gacgttctcg aggatcgtca tcgacgacca cagcgccgcg ctctggaaca gcatgccgaa
     1561 tccggtgcgc aggcgcgccc ggccggcctc gtcggcgcgc cagtagtcga cgctgtcgta
     1621 ggtcacgctg cccttggccg gccgcagcag gccgatcagg tgcttcagca gcgtgctctt
     1681 gccgcagccg ctgccgccca tgatcgcgaa gatcgaaccc ttttcgacct tgaagctcac
     1741 gccggtctga atcaggcgct cgccgaagcg catctccagg tcgtcgacgg cgagcagggt
     1801 catgggctca gatccccagc gcgttcgcgc agaccgcgaa caccgcgtcg agcacgatga
     1861 tgccgacgat gctgacgacg accgcgttgg tcgtcgccac gcccacgccc tcggcgttgc
     1921 gctgcgcgta caggccgaag tagcagccga ccatgccgat cagcgcgccg aacacgatcg
     1981 acttcgagaa gcccagcccg agatgcgacc agtccagcgc cacgagcgtg cggtccaggt
     2041 aggcggcggg cgagatgtcg agcatgcccg cggcgacgag catgccgccg agcagcccgg
     2101 tgacgcagcc gtagacgtac agcagcggca tcatcagcag cagcgccatc acgcgcggca
     2161 gcaccaggaa gtcgaccgcg ctcaagccga gcacctcgag cgcatcgacc tcttcgttgg
     2221 cctgcatcgt cgcgagctcg gcggcgaacg cagcgccggt gcgccccgcc atcacgacgg
     2281 cggtgatgat cgcggccatc tcgcgtgcca ccgcgatgcc gaccaggtcg gccacataga
     2341 tgccggcgcc gaacttgacg agctgcaccg cgccgacgaa cgccaggatc gccccgacca
     2401 gcacgttgac gacggtgacg atcggcaccg cgcgggcgct gcagtcggcg gtgatgcggc
     2461 ccaggtcctt gagacggaac tgccaccggc ccagcacggc ctcgacggcg gagatcagca
     2521 cctggccgag gaagacgacc ggccggatcg ggaggagcga ggcgatctgc atgggggctg
     2581 atcggcattg tgccgcgcgg cgtccgtctg cactcgacct ctctcgagcg gcgttgcgca
     2641 tcgggaccgg accctacaat cgtttcaaca cgatcaaccg cggcccgacc atgaccaccg
     2701 acgctgctgc cgccccggcg gccgacgccc ccgacctgtc gcacatctcg ccgcgcgaca
     2761 aggccgagat cctcgcgcag gcactgccct acatccgcaa gttccacggc aagaccatcg
     2821 tcatcaagta cggcggcaac gcgatgaccg acccgggcct gcagcaggat ttcgccgagg
     2881 acgtggtgct gctgaagctg gtcggcatga accccgtggt ggtgcacggc ggcgggccgc
     2941 agatcgacga ggccctgagc cgcttgggca agaagggcac cttcatccag ggcatgcgcg
     3001 tgacc
//
LOCUS       DATMJB010000469         2322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_507234, whole genome shotgun sequence.
ACCESSION   DATMJB010000469 DATMJB010000000
VERSION     DATMJB010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507234"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..224
                     /locus_tag="VNU48_10015"
                     /pseudo
     CDS             1..224
                     /locus_tag="VNU48_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180478.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(360..2087)
                     /locus_tag="VNU48_10020"
     CDS             complement(360..2087)
                     /locus_tag="VNU48_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HWI11654.1"
                     /translation="MYVAIVPNRNSPPAVLLRESFRDGDKVKNRTLTNLSHWSADKIA
                     ALRAVLRGDRLVPVGEGLEIVRALPYGHVAAALGVARRIGLDRLVTGPRRKRDLILAL
                     IIARLIEPAAKLATARALDEATASSALGLGLGLGAVTATEIYDALDWLGAQQDQIEVT
                     LAKRHLAGATLVLYDLTSTYLEGRCCPLAHHGHSRDGRPDKLQIVFGVICSQDGCPIA
                     VEVFAGNTGDPSTLKAQIDKLKTRFGLTRIALVGDRGMITQARIDQDIVPAGLDFITA
                     LRAPAIRALAEDGGPLQLSLFDERDLAEIDSPDYPGERLVACRNPVLGQERTRKRDEL
                     LDATERDFQRIQARVRRVRQPLRGAAAIGAAVGAVLNRRKMAKHFDMTITDTDFAFVR
                     NQASIDAEARLDGIYVLRTTLAQDRLDTAAVVLAYKGLARVERAFRSLKTVDIEVRPV
                     FHYAGDRVRAHVFLCMLAYYLEWHMRQALAPILFDDHDRAAAKAQRVSPVARARISPA
                     ARRKAKTQRTDDGLPVHSFRSLLADLATLTRNQVRFGSGNTFTMLATPTPVQQRTFDL
                     LGVRPGAGL"
BASE COUNT          360 a          704 c          858 g          400 t
ORIGIN      
        1 agatcgtcgc cttccgcctg atgcagggcc tgttcggcgc atcgctggtg ccgctttccc
       61 agggcatcct cctggacatc tataccgtcg aggagcgcgg ctcggccatg gcccttttcg
      121 gcgtctcggt tatggtcggg ccggtgcttg ggcctgtcat cggcggctgg ctgaccgaca
      181 acatcaattg gcgctgggtg ttttatatca acgtgccgat cggcgttctc gccttcatcg
      241 gcatcgtctt cttcgtgacg gaaaccaagg tcgatgcgct agccggaact ttagcttgtt
      301 gagagtgcta aggtcttgac gcggcttgcg tttgcgagca gcggcgtgcg gcgtactgac
      361 tacaaaccgg ccccgggtct gacaccgagg aggtcgaagg tgcgttgctg caccggggtt
      421 ggggtggcaa gcatggtgaa ggtgttgccc gagccgaagc ggacctggtt gcgggtgagc
      481 gtggcgaggt cggcgaggag cgagcggaag ctgtggacgg gcaggccgtc gtcggtgcgc
      541 tgggtcttgg ccttgcgccg ggcggcaggg gagatgcggg ccctggcgac gggcgagacg
      601 cgctgggcct tggccgcggc gcggtcgtga tcgtcgaaca ggatgggggc gagcgcctgg
      661 cgcatgtgcc attcaagata ataggcgagc atgcacaaga agacatgggc gcggacccgg
      721 tctccggcat aatggaacac cggccgcacc tcgatgtcga cggtcttgag gctgcggaag
      781 gctcgttcga cccgggcgag gcccttgtag gccaggacga cggcggcggt gtcgagcctg
      841 tcctgcgcaa gggtggtgcg cagcacatag atgccgtcga gcctggcttc ggcgtcgatc
      901 gaggcctggt tgcggacgaa ggcgaagtcg gtgtcggtga tggtcatgtc gaagtgcttg
      961 gccatcttgc ggcggttgag cacggcaccg accgccgcgc cgatggcggc cgccccgcgc
     1021 agcggctggc gcacgcggcg cacgcgcgcc tgaatgcgct ggaaatcgcg ttcggtggca
     1081 tcgagcagtt cgtcgcgctt gcgggtgcgc tcctggccga ggaccggatt gcggcaggcc
     1141 accaggcgct cgccgggata gtccgggctg tcgatctcgg ccaggtcgcg ctcgtcgaac
     1201 agcgacagct gcaagggccc gccgtcctcg gccagcgccc ggatggcggg cgcccgcaac
     1261 gcggtgatga agtcgaggcc ggccgggacg atgtcctgat cgatccgggc ctgggtgatc
     1321 atgccgcgat cgccgacgag ggcgatgcgc gtcaggccga agcgggtctt gagcttgtcg
     1381 atctgggctt tgagcgttga cgggtcgccg gtgttgccgg cgaacacctc gacggcgatc
     1441 gggcagccgt cctggctgca gatcacgccg aacacgatct gcagcttgtc ggggcgtccg
     1501 tcgcggctgt ggccgtggtg ggccagcgga cagcagcggc cctccagata ggtcgaggtc
     1561 aggtcgtaga ggaccagggt ggccccggcc agatgccgct tggcgagcgt gacctcgatc
     1621 tgatcctgct gggcgccgag ccagtcgagc gcatcgtaga tctcggtggc ggtgacggcg
     1681 cccaggccga gccccaggcc cagggcggag ctggcggtgg cctcgtcgag ggcgcgggcg
     1741 gtggccagct tggcggcggg ctcgatcagg cgggcgatga tcagcgccag gatcaggtcg
     1801 cgcttgcgtc gcggcccggt gacgaggcgg tcgagcccga tccgccgggc cacgccgagc
     1861 gccgcggcga cgtggccgta cggcagggcg cggacgatct ccaggccctc gccgaccggc
     1921 accagcctgt cgccgcgcag cacggcgcgc agcgccgcga tcttgtcggc cgaccagtgg
     1981 ctcagattgg tgagcgtgcg gttcttgacc ttgtcgccgt cgcggaagct ctcgcgcagc
     2041 agcacggcgg ggggcgagtt gcggttgggg acgatggcga cgtacatgcg cgaatcgaat
     2101 catctccgcc tgccgccgtc aaccgaaaaa cacaatttac atgactacaa tccactggaa
     2161 aaatcccact ttccacagca aatccaacgc cttggctcaa aaggcagggt aaagttccgt
     2221 ttagacgctt cataacatga taggaatatt ttctcatcgc aacggcgacc ggatccactc
     2281 gcgccggtgc cgcctttcgg tggcccagac gacggcggcg cc
//
LOCUS       DATMJB010000470         2374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_8318, whole genome shotgun sequence.
ACCESSION   DATMJB010000470 DATMJB010000000
VERSION     DATMJB010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8318"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VNU48_10025"
     CDS             <1..321
                     /locus_tag="VNU48_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HWI11655.1"
                     /translation="GPVGRLPCKFNLIPFNPFPESGLRCSPRERVLAFADVLSQAGLV
                     TTVRKTRGDDIDAACGQLAGEVQDRTNAKVRMTRAPVQLHRRGSAAAEAIRTGRAGLQ
                     ETAP"
     gene            318..1142
                     /gene="pilW"
                     /locus_tag="VNU48_10030"
     CDS             318..1142
                     /gene="pilW"
                     /locus_tag="VNU48_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HWI11656.1"
                     /translation="MTMPWMPGRTRRWLPALLLSAALAACATRSTVTQTVGDGKDIVT
                     ASDESDAGKRARLRMELASGYFGRGQMTTALDQVKLALQADPTLGEAYNLRGLIYANL
                     GDDKLAEESFRRALQLNPRDADSMQNFGYYLCQKKRYPEASALFDEALAVPQYRDQAR
                     TLLTKGVCEAFAGKLPESEATLLRAYQLDPANPSISVNLSEVLYRRGDYERARFYIRR
                     VNAVPQFTGAQTLWLAARVEHRLGNVSGAQELGDQLRHRFPESREAGSFERGQFDE"
     gene            1135..2019
                     /locus_tag="VNU48_10035"
     CDS             1135..2019
                     /locus_tag="VNU48_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HWI11657.1"
                     /translation="MSESPAPAAAPPAPATTAGCLLRQARQAQGLHIAALAAAIKVVP
                     RKLELLESDQFDQLPDPTFTRALAQTICRTLKIDAAPILALLPPPKGHRLEHVAGGLN
                     TPFRERPGNMAPKEWASVKNPVLWIVAAIALLTLAVYAVPASWLPFSTAAVTRVRPPE
                     TAVRPAALSAPAVALPVAASAPPSTPVPADVAASGTPVAEPPAALTEPDAVTAAAASG
                     VLQFSASAQSWVEVTDARGQSLIARVLEPGESVGLDGVMPLKVKVGNAAGTQLVFRGQ
                     PLALAAYTRDNVARLELK"
     gene            2040..>2374
                     /locus_tag="VNU48_10040"
     CDS             2040..>2374
                     /locus_tag="VNU48_10040"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWI11658.1"
                     /translation="MSDKTPTFPELDDYVEPAVPAAHRTRQAKVVWGSRIVTVGGGAP
                     VRVQAMTNTDTVDAIGTAIQVKELAAAGSELVRITVNTPEAAAAVPYIRDQLDRMVVD
                     VPLIGDFHY"
BASE COUNT          352 a          856 c          848 g          318 t
ORIGIN      
        1 ggcccggttg gccgcctgcc gtgcaagttc aacctgatcc cgttcaaccc gtttcccgag
       61 tccggcctgc gctgctcgcc gcgcgagcgt gtgctggcct ttgctgacgt gctgagccag
      121 gccgggctgg tcaccaccgt gcgcaagacg cgcggtgacg acatcgacgc cgcatgcggc
      181 cagctcgccg gcgaagtcca ggaccggacc aacgccaagg tccgcatgac gcgggcaccg
      241 gtgcagctgc accgccgcgg ttcggcggcg gccgaggcga tcaggaccgg acgcgccggc
      301 ctgcaggaga ctgcgccatg acgatgccgt ggatgcccgg ccgcacgcgg cgctggctgc
      361 ccgcgctgct gctgagcgcc gcactcgcgg cgtgcgcgac ccgcagcacg gtgacccaga
      421 cggtgggcga cggcaaggac atcgtgaccg cctccgacga atcggacgcg ggcaagcgcg
      481 cgcgcttgcg catggaactg gccagcggct acttcggccg tggccagatg accaccgcgc
      541 tggaccaggt caagctcgcg ctgcaggccg atccgacgct gggcgaggcc tacaacctgc
      601 gcggcctgat ctacgccaac ctgggcgacg acaagcttgc cgaagagagt ttccggcgcg
      661 cgctgcagct gaacccgcgc gacgccgact cgatgcagaa cttcggctac tacctgtgcc
      721 agaagaagcg ctaccccgag gcgagcgcct tgttcgatga ggccctcgcg gtgccgcagt
      781 accgcgacca ggcgcgcacc ttgctgacca agggcgtgtg cgaggccttc gccgggaagc
      841 tccccgagtc cgaggcgacg ctgctgcgcg cctaccagct cgatccggcg aacccgtcga
      901 tctcggtgaa cctgtccgag gtgctgtacc ggcgcggcga ctacgagcgc gcgcgcttct
      961 acatccgccg cgtcaacgcg gtgccgcagt tcaccggcgc gcagacgctg tggctcgcgg
     1021 cgcgcgtcga gcatcggctg ggcaacgtat cgggcgcgca ggaactcggc gaccagctgc
     1081 gtcaccgctt ccccgagtcg cgcgaggcag ggtcgttcga gcgaggtcag ttcgatgagt
     1141 gaatcgcccg caccggccgc ggcgccgccc gccccggcga ccaccgcggg ctgcctgctg
     1201 cgccaggcgc gccaggcgca gggcctgcac atcgcggccc tcgcggcggc gatcaaggtc
     1261 gtgccgcgca agctcgagct gctggaatcc gaccagttcg accagttgcc cgaccccacc
     1321 ttcacgcgcg cgctcgcgca gaccatctgt cgcacgctga agatcgacgc ggcaccgatc
     1381 ctggcgctgt tgccgccgcc gaaagggcac cggctcgagc atgtcgccgg gggcctgaac
     1441 acgccgttcc gcgaacgccc ggggaacatg gcgccgaagg agtgggcgag cgtcaagaac
     1501 ccggtgctgt ggatcgtcgc cgcgatcgcc ctgttgacgc tggcggtgta tgcggtgccg
     1561 gcgagctggc tgccgttctc gactgccgcg gtgacgcgcg tgcgcccgcc cgaaaccgcc
     1621 gtcaggccgg ccgcgctgtc ggctccggcg gtggcgcttc cggtggcggc gtcggcgccg
     1681 ccgagcacgc cggtgccggc ggacgtggcg gcctccggca caccggtggc cgagccaccg
     1741 gccgcgctga ccgagcccga tgcggtcacc gccgcggccg cctcgggcgt gctgcagttc
     1801 agcgcgtccg cgcagtcctg ggtcgaggtg accgatgcgc gcggccagtc gctgatcgca
     1861 cgcgtgctcg agccgggcga aagcgtcggc ctcgacggcg tgatgccgct gaaggtcaag
     1921 gtcggcaatg cggccggcac gcagctggta tttcgcggtc agccgctggc gctggccgcc
     1981 tacacgcgcg acaacgtggc gcggcttgaa ctgaagtagc agcgcagggc ggtgacacca
     2041 tgagcgacaa gaccccgaca ttccccgagc tcgacgacta cgtcgaaccc gccgtgccgg
     2101 ccgcgcaccg cacgcggcag gcgaaggtgg tgtggggctc gcgcatcgtg acggtgggcg
     2161 gcggcgcgcc ggtgcgcgtg caggcgatga ccaacaccga cacggtcgac gcgatcggca
     2221 ccgcgatcca ggtcaaggag ctggccgccg cgggttcgga gctggtgcgc atcaccgtca
     2281 acacgcccga ggccgcggcg gccgtgccct acatccgcga tcagctcgac cgcatggtcg
     2341 tggacgtgcc gctgatcggc gacttccact acaa
//
LOCUS       DATMJB010000471         4305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_7861, whole genome shotgun sequence.
ACCESSION   DATMJB010000471 DATMJB010000000
VERSION     DATMJB010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7861"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VNU48_10045"
     CDS             complement(<1..223)
                     /locus_tag="VNU48_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HWI11659.1"
                     /translation="MTASTHRFRFRAASLTALALAAALTVAVRAADDKAAASAPARVA
                     LSVNVTQPQTTALPLRINANGNIAAWQEAS"
     gene            complement(220..1659)
                     /locus_tag="VNU48_10050"
     CDS             complement(220..1659)
                     /locus_tag="VNU48_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWI11660.1"
                     /translation="MTTPWFCPSRAVLAVATLTGLAACSNGPPPQPIAATAEADLPAQ
                     WQAPLPHHGELAQLTSWWQQFDDPLLPRLIAAAQAASPTVASAASRIEQSRATRVAAG
                     AALLPALDANASLVRGRQDFISGLGNSASAVLQASWELDLFGGRRASREAAQARFDGA
                     QAGWHDARVAVAAEVATDYTALRACEAQVEQTRLDSGSRSETARLTDLSTKAGFESPA
                     NAALARASAAQGNSLLTQRRAQCDVLVKGLVALTGIAEPALRSALTSRRAQVPAPAQI
                     ALTSVPAQVLAQRPDVRSAGRELVAASAEVTQAQAARLPRIMLNGSLGATRVESADFG
                     NQSGTLWAIGPVTVSLPLFDAGTRRANVSAAQARYDEAGALYRARLRSAVREVEEALV
                     ALQSTDDRSTDARIATEGFAASFRATEARYRGGLASLYELEDARRSALQAQIALIDLQ
                     RERSTAWISLYRALGGGWSGNDNSVATNP"
     gene            complement(1862..2962)
                     /locus_tag="VNU48_10055"
     CDS             complement(1862..2962)
                     /locus_tag="VNU48_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11661.1"
                     /translation="MTTTLNLIDLQSGLPHTGLTAHEHVGFELGWDYAHYRVAPPAPY
                     AHEPSPLRDGLLAGQAAFGARTLAVTRHVRKWLQLRLHAWLRGRSVELVQVTPNYLQQ
                     IDASHCPITRVALSSATLEASDASVDRVRNDAGYAAGNLAVMSTKANHAKGAHGFRDA
                     TRFVQQIEAEQLPGLGGLTAEQWSRVAVLCSFVEPLPFEEACALPLLVLPPNRLRLFN
                     PAQALQAFVSQQLLKPGWSQRIGRFEDLLPGKALRRDFRSFFMSLLPRVIEAGRADTA
                     HARWAIEDAWRASLVQQRWTRFARQLGAAQCEALLARAAARKLGAGVLLPLTDASATD
                     GWNLATRGYLPHAANVVHGDAARDQRQQQLAW"
     gene            3126..4169
                     /locus_tag="VNU48_10060"
     CDS             3126..4169
                     /locus_tag="VNU48_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HWI11662.1"
                     /translation="MDRTERFYKIELLLKSRGCIGFAALQDELAVSPATLKRDLQYLR
                     DRLSAPIVYDAFANGYRFAEAKGDALGAARHELPGLWFSENEIHALLTMHQLLAGLDD
                     DGVLARHLQPLMEKLQGMLGTDESAARELMRRVKVIGTARRRAPSRHFELLGSALLQR
                     RRLRLRYFKRSDRSESEREVSPQRLVNYRNTWYLDAWCHASDGLRRFALDAMRAAALL
                     DAKARHVPLRELEAALDAGYGIYGGGGAKLRWATLVFDADAAQWVANEEWHPQQKARW
                     LGGRAGEGRYELQVPYSDATELAMDILRHGDSVLVTGDKALAALIGERLRSAAARYAG
                     SLPKQVEQRKDHT"
     gene            4166..>4305
                     /locus_tag="VNU48_10065"
     CDS             4166..>4305
                     /locus_tag="VNU48_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HWI11663.1"
                     /translation="MKATWNGVTIAESDDTVIVEGNHYFPEASLKREYVSFSNHRSSC
                     GW"
BASE COUNT          599 a         1524 c         1599 g          583 t
ORIGIN      
        1 cgctggcctc ttgccacgcg gcgatgttgc cgttcgcgtt gatgcgcagc ggcagcgccg
       61 tcgtctgcgg ctgcgtcacg ttgacgctga gcgccacgcg cgccggcgcc gaggccgccg
      121 ccttgtcgtc ggccgcgcgc accgcgaccg tcagcgcggc cgcgagcgcg agggccgtca
      181 atgaggcggc gcggaacctg aagcgatggg tcgatgcggt catgggttgg tggccactga
      241 gttgtcgttg ccggaccagc cgcccccgag ggcgcggtac agcgagatcc aggcggtgct
      301 gcgctcgcgc tgcaggtcga tcagcgcgat ctgcgcctgc agcgccgagc ggcgcgcgtc
      361 ttcgagttcg tacaggctcg ccaggccgcc ccggtagcgc gcctcggtgg cgcggaacga
      421 cgcggcgaag ccctcggtgg cgatgcgcgc gtcggtgctg cgatcgtcgg tgctctgcag
      481 cgcgaccagt gcctcctcga cctcgcgcac cgcgctgcgc agccgggcac ggtacagcgc
      541 gccggcctcg tcgtagcgtg cctgtgccgc gctcacgttg gcgcggcgcg tgccggcgtc
      601 gaacagcggc agcgacaccg tgaccgggcc gatcgcccac agcgtgccgc tctggttgcc
      661 gaagtcggcc gactcgacac gtgtcgcgcc gaggctgccg ttcagcatga tgcgcggcag
      721 ccgcgcggcc tgcgcctgcg tgacctcggc gctcgcggcg accagctcgc gcccggcgct
      781 gcgcacgtcg gggcgctgcg ccagcacttg cgccggcacg ctggtcaacg cgatctgcgc
      841 cggtgccggc acctgcgccc ggcggctcgt cagcgcgctg cgcagcgcgg gctcggcgat
      901 gccggtcagc gcgaccaggc ccttgaccag cacgtcgcac tgtgcgcggc gttgcgtcag
      961 caggctgttg ccctgcgccg cgctggcgcg tgcgagcgcg gcgttggcgg gcgattcgaa
     1021 accggccttc gtgctcaggt cggtcaggcg cgcggtctcg ctgcgcgagc cgctgtccag
     1081 ccgcgtctgc tcgacctgcg cctcgcaggc gcgcagcgcg gtgtagtcgg tcgcgacctc
     1141 ggccgcgacc gcgacgcgcg catcgtgcca gccggcctgc gcgccgtcga agcgcgcctg
     1201 cgcagcctcg cgcgaggcgc ggcggccgcc gaacaggtcg agctcccagc tcgcctgcag
     1261 gacggcactg gcgctgttgc ccaggccgct gatgaaatcc tgcctgccgc gcaccaggct
     1321 ggcgttcgcg tccagcgccg gcagcagcgc cgcgcccgcg gcgacgcggg ttgcgcgcga
     1381 ctgctcgatg cgcgacgcgg ccgaggccac cgtcgggctc gcggcctgcg ccgcggcgat
     1441 caggcgcggc agcagcgggt cgtcgaactg ctgccaccag ctggtcagct gggcgagctc
     1501 gccgtgatgc ggcagcggtg cctgccattg cgccggcaga tcggcctctg cggtggcggc
     1561 gatcggctgc ggcggcgggc cgttgctgca ggctgcgagg ccggtgagtg tcgccacggc
     1621 caagacggcg cgcgacgggc agaaccaggg ggtcgtcatc acgaatcgtt ttcgctttct
     1681 cgcacgcggg tgaggtgctc gcaccctgcg ccgcgcggcg cgtgcgggtc gcacggcggg
     1741 cgggcaagcc gcgagggacc aatataacaa cgtgacgacg aacggcgcgc gcgcaaatcg
     1801 acacggccgc aaggctgctg acaacggggt gtcagcagcc tgcggcccgg cggccggcac
     1861 gctaccaggc gagttgctgc tggcgctggt cgcgcgcggc gtcgccgtgc acgacgttcg
     1921 cggcatgcgg caggtagccg cgcgtggcga ggttccagcc gtcggtcgcg ctggcgtcgg
     1981 tcagcggcag cagcacgcca gcgccgagct tgcgcgccgc ggcccgggcc agcagcgcct
     2041 cgcactgggc ggcgccgagc tggcgcgcga agcgggtcca gcgctgctgc acgagcgacg
     2101 cgcgccaggc gtcctcgatc gcccagcgcg cgtgcgccgt gtccgcacga ccggcctcga
     2161 tcacccgcgg cagcaggctc atgaagaagc tcctgaaatc gcgccgcagc gccttgccgg
     2221 gcagcaggtc ctcgaagcgg ccgatgcgct ggctccagcc cggcttgagc agctgctggc
     2281 tgacgaaggc ctgcagcgcc tgcgccgggt tgaacaggcg cagccggttc ggcggcagca
     2341 ccagcagcgg cagcgcgcag gcctcctcaa acggcaaggg ctcgacgaag ctgcacagca
     2401 ccgcgacgcg cgaccactgc tcggcggtca ggccgcccag gcccggcagc tgttcggcct
     2461 cgatctgctg cacgaagcgc gtcgcgtcgc gaaagccgtg cgcgcccttc gcgtggttcg
     2521 ccttggtgct catcactgcg aggttgccgg ccgcgtagcc ggcgtcgttg cggacgcgat
     2581 cgaccgaggc atcgctcgcc tcgagcgtgg cgctggacag cgcgacgcgc gtgatcgggc
     2641 agtggctcgc gtcgatctgc tgcaggtagt tcggcgtgac ctgcacgagt tcgacactgc
     2701 gcccgcgcaa ccacgcgtgc agccgaagct gcagccactt gcgcacatgg cgcgtcaccg
     2761 ccagcgtgcg cgcgccgaac gcggcctggc cggcgagcag cccgtcgcgc agcggcgacg
     2821 gctcgtgcgc gtacggcgcg ggcggggcga cgcggtagtg cgcgtagtcc cagccgagct
     2881 cgaagccgac gtgctcgtgc gccgtcaggc cggtgtgggg aaggccgctt tgcaggtcga
     2941 tcaggttcag ggtcgtggtc atggtgtggc tccgcgaggt tcgccgcgaa ccgttcgcag
     3001 cgatggggcc agtgtcgcgc ccaggtagct catggcgtga gctaccaaag gcgaattcga
     3061 ctcggggtat gctctgcgcc actggctcac gccatgagcc acctacttga tctcggatca
     3121 ccgacatgga ccgcaccgaa cgcttctaca agatcgagct gctgctgaaa agccgcggct
     3181 gtatcggctt cgctgcgctg caggacgagc tcgccgtgtc gcccgcgacg ctgaagcgcg
     3241 acctgcagta cctgcgcgac cgcctcagcg caccgatcgt ctacgacgcg ttcgcgaacg
     3301 gctaccgctt cgccgaagcg aagggcgacg cgctcggcgc ggcacgccac gagctgccgg
     3361 ggctgtggtt cagcgagaac gagatccacg cgctgctgac gatgcaccag ctgctcgccg
     3421 gcctggacga cgacggcgtg ctggcgcgcc acctgcagcc gctgatggag aagctgcagg
     3481 gcatgctcgg caccgacgag agcgcggcgc gcgagctgat gcgccgcgtc aaggtcatcg
     3541 gcaccgcgcg gcggcgcgcc ccgagccggc acttcgagct gctcggcagc gcgctgctgc
     3601 agcgccgacg cctgcggctg cgctacttca agcgcagcga ccgcagcgag agcgagcgcg
     3661 aggtctcgcc gcagcgcctg gtgaactacc gcaacacctg gtacctcgac gcgtggtgcc
     3721 atgccagcga cggcctgcgc cgtttcgcgc tcgacgcgat gcgcgcggcg gcgctgctgg
     3781 acgcgaaggc caggcacgtg ccgctgcgcg aactcgaggc cgcgctcgac gccggctacg
     3841 gcatctacgg cggtggcggc gcgaagctgc gctgggcgac gctggtgttc gacgccgacg
     3901 ccgcgcagtg ggtcgcgaac gaggaatggc acccgcagca gaaggcgcgc tggctcggcg
     3961 gccgggcggg cgagggccgc tacgagctgc aggtgccgta cagcgacgcg accgaactcg
     4021 cgatggacat cctgcgccat ggcgacagcg tgctcgtgac cggcgacaag gcgctggccg
     4081 cgctgatcgg cgaacgcctg cgcagcgccg cggcgcgata tgcgggatca ttgccgaagc
     4141 aagtcgagca gcgaaaggac cacacgtgaa ggccacctgg aacggcgtga ccatcgccga
     4201 gagcgacgac acggtgatcg tcgaaggcaa ccattacttt cccgaggcga gcctgaagcg
     4261 cgaatacgtc agcttcagca accaccgcag cagctgcggg tggaa
//
LOCUS       DATMJB010000472         3546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_220805, whole genome shotgun sequence.
ACCESSION   DATMJB010000472 DATMJB010000000
VERSION     DATMJB010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220805"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1824)
                     /locus_tag="VNU48_10070"
     CDS             complement(82..1824)
                     /locus_tag="VNU48_10070"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HWI11664.1"
                     /translation="MSQDSTPKKKPADLRSQQWFGRQDRDGFNYRSWVKGKGVPHDQF
                     DGRPVIGICNTFSELTPCNSHFRTLAEQVKIGVYEAGGFPLEFPVMSLGETLLRPTAM
                     LYRNLASMDVEESIRGNPIDGVVLLMGCDKTTPALVMGAASVDLPTIGVSGGPMLNGK
                     WRGQELGSGTGVWSMSEQVRAGSLKLQDFFEAESCMHRSHGHCMTMGTASTMASMVEA
                     LGIGLPGNAAYPAVDGRRNVLARMAGRRIVEMVHSDQKLSSILTREAFENAVRTLAAI
                     GGSTNAVIHLIAIAGRLGMKLTIDDFDRLASELPCLVNLQPSGKYLMEDFCYAGGLPA
                     LMREIAAWLHLDHVTASGRTLRENIADARNDNPAVIHSIDNPFKANAGIAVLRGNLAP
                     RGAIIKPSAATPALMKHRGRAVVFDTIEEFHAKIDDESLDIDESCVMVLRNCGPKGYP
                     GMAEVGNMPLPPKVLRKGITDMVRISDARMSGTAYGTVVLHTAPEAAAGGPLAAVRSG
                     DFIELDVAQRRLHLDVGDAEIAARLRDWTAPPPPLGSGYWKLYVDHVLQADQGVDLDF
                     LVGRRGAFVPRDNH"
     gene            1926..2873
                     /locus_tag="VNU48_10075"
     CDS             1926..2873
                     /locus_tag="VNU48_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HWI11665.1"
                     /translation="MPDIEILSVATPPPFLLEPLQAKYVVHQRLHESDPAAFASVAPK
                     IRAIAASGESKVGAALIAQLPALEVISVMGVGYDGIDVAAAKARGVVVTHTPAVLNDD
                     VADLAIALMLAWSRRIARADRFVRAGEWPKGPLPLGRKMSGARLGLVGMGRIGQAIAL
                     RAAAFGMTIAYTARSAKGELPHTYFPSAAALAAHSDYLVVITPGGAGTRHLIDAAVLA
                     ALGPQGCLVNVARGSVVDEAALIEALHAGTVAGAALDVFENEPNVPQALREMDQVVLT
                     PHIGSATGQTRRAMAALAFDNLDARLSGRPVLTPVPECR"
     gene            2974..>3546
                     /locus_tag="VNU48_10080"
     CDS             2974..>3546
                     /locus_tag="VNU48_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWI11666.1"
                     /translation="MKPKTWIAAALAGAALMGAAPVMAQDKLTVWWVKGFYKSEDDAL
                     FAAIRKFEAKHPKVKIELSQYPVQDMMPKTVAALDSGNPPDVAYADIYDFQATGKWAF
                     DGKLEDITSVIDPIRARFAPNTIETTFLYNDKTKSRAYYAFPIKQQTMHIEYWLDMLT
                     DAGFKESDIPTTWKEYWSFWCDKVQPAYRQK"
BASE COUNT          548 a         1231 c         1213 g          554 t
ORIGIN      
        1 gcgctgttca cggtggcgtc gggcacgcgc gccaggcgct gacgagcgac gcgctgccct
       61 ggtcgacgcc gacccaggtc gtcagtggtt gtcgcgcggc acgaacgcgc cgcgccggcc
      121 gacgaggaag tcgaggtcga caccctgatc ggcctgcagc acatggtcga cgtagagctt
      181 ccagtagccg ctgcccagcg gcggcggcgg ggccgtccag tcgcgcaggc gggcggcgat
      241 ctcggcgtcg ccgacgtcga ggtgcagccg gcgctgcgcg acgtcgagct cgatgaagtc
      301 gccgctgcgc accgccgcga gcgggccgcc ggcggcggcc tcgggcgcgg tgtgcagcac
      361 gacggtgccg tacgcggtgc cgctcatgcg cgcgtcgctg atgcggacca tgtcggtgat
      421 gcccttgcgc aggaccttcg gcggcagcgg catgttgccg acctcggcca tgccggggta
      481 gcccttcggc ccgcagttgc gcagcaccat cacgcagctc tcgtcgatgt cgagcgactc
      541 gtcgtcgatc ttcgcgtgga actcctcgat cgtgtcgaac accaccgcgc ggccgcggtg
      601 cttcatcagc gccggcgtcg cggcactcgg cttgatgatc gcgccgcgcg gcgcgaggtt
      661 gccgcgcagc accgcgatgc cggcgttggc cttgaacggg ttgtcgatcg aatggatcac
      721 cgccgggttg tcgttgcgtg catcggcgat gttctcgcgc agcgtgcggc cgctcgcagt
      781 gacgtggtcg aggtgcagcc acgcggcgat ctcccgcatc agcgccggca gcccgccggc
      841 gtagcagaag tcctccatca ggtacttgcc cgagggttgc aggttcacca ggcacggcag
      901 ctcgctcgcg aggcggtcga agtcgtcgat cgtgagcttc atgccgagcc gcccggcgat
      961 cgcgatcagg tggatcaccg cgttggtcga gccgccgatc gcggcgagcg tccgaacagc
     1021 gttctcgaac gcctcgcggg tcaggatcga cgagagcttc tggtcgctgt gcaccatctc
     1081 gacgatgcgc cgcccggcca tccgcgcgag cacgttgcgc cgcccgtcga ccgccgggta
     1141 cgcggcgttg cccggcagcc cgatgcccag cgcctcgacc atgctggcca tcgtgctggc
     1201 ggtgcccatc gtcatgcagt ggccgtggct gcggtgcatg cagctctcgg cctcgaagaa
     1261 gtcctgcagc ttcagcgagc cggcgcgcac ctgctcgctc atgctccaca cgccggtgcc
     1321 cgagccgagc tcctggccgc gccacttgcc gttgagcatc ggcccgcccg acacgccgat
     1381 cgtcggcagg tcgacgctgg ccgcgcccat caccagcgcc ggcgtggtct tgtcgcagcc
     1441 catcagcagc acgacgccgt cgatcgggtt gccgcggatg ctctcttcca cgtccatcga
     1501 cgcgaggttg cggtacagca tcgcggtcgg ccgcagcagc gtctcgccca ggctcatcac
     1561 cgggaattcg agcgggaacc cgccggcctc gtagacgccg atcttgacct gctcggcgag
     1621 cgtgcggaag tgcgagttgc acggcgtgag ttcgctgaag gtgttgcaga tgccgatcac
     1681 cggccggccg tcgaactggt cgtgcggcac gcccttgccc ttgacccagc tgcggtagtt
     1741 gaagccgtcg cggtcctggc ggccgaacca ctgctggctg cgcaggtcgg cgggcttctt
     1801 cttcggcgtc gaatcctggc tcatgagact gcgcggtttc ccggtggctg tgaaagaatg
     1861 gctgtcatat tatggtacga cgattgagtt cgtcggccat ccgctgaaac ccgggagcgc
     1921 caaccttgcc cgacatcgag atcctgtccg tcgcgactcc gccgccgttc ctgctcgaac
     1981 cgctgcaggc gaagtacgtg gttcaccagc gcctgcacga gtccgacccg gcggcgttcg
     2041 cgagcgtcgc gccgaagatc cgcgcgatcg cggccagcgg cgaatcgaag gtcggcgccg
     2101 cactgatcgc gcagctgccg gcgctcgagg tgatctcggt gatgggcgtc ggctacgacg
     2161 gcatcgacgt ggccgcggcc aaggcgcgcg gcgtcgtcgt cacgcacacg ccggcggtgc
     2221 tgaacgacga cgtcgccgac ctggcgatcg cgctgatgct ggcctggtcg cgcagaatcg
     2281 cgcgcgccga ccgcttcgtg cgcgccggcg agtggccgaa ggggccgttg ccgctcgggc
     2341 gcaagatgtc cggggcgcgc ttggggctgg tcggcatggg ccgcatcggc caggcgatcg
     2401 cgctgcgcgc cgccgccttc ggcatgacga tcgcgtacac cgcgcgcagc gcgaagggcg
     2461 agttgccgca cacgtatttc ccgagcgccg cggcgctggc cgcgcacagc gactacctgg
     2521 tcgtcatcac gcccggcgga gcgggtacgc gtcatctgat cgacgccgcg gtgctggccg
     2581 cgctcggccc gcagggctgc ctggtcaatg tcgcgcgcgg ctcggtggtc gacgaagcgg
     2641 cactcatcga ggccttgcac gccggcaccg tcgccggcgc cgcgctcgat gtgttcgaga
     2701 acgagccgaa cgtgccacag gcgctgcgcg agatggacca ggtcgtgctg accccgcaca
     2761 tcggcagcgc caccggccag actcgccggg cgatggccgc gttggccttc gacaacctcg
     2821 acgcccggct cagcggccga ccggtgttga cgccggtgcc cgagtgccgc tgatcgatga
     2881 gctgcgggaa agcccgcagg gtcgatttcg ccaaacatca tacgatcaca cgataacgcc
     2941 cacctgaccg gcgccgccgg aggagacacg cagatgaagc cgaagacctg gatcgccgcc
     3001 gccctggccg gtgccgcact gatgggggcc gcacccgtga tggcccagga caagctcacc
     3061 gtctggtggg tcaagggctt ctacaagtcc gaggacgacg cactcttcgc ggcgatcagg
     3121 aagttcgagg ccaagcaccc gaaggtcaag atcgagctgt cgcagtaccc ggtacaggac
     3181 atgatgccca agaccgtcgc ggcgctcgat tcaggcaatc cacccgacgt agcctacgcc
     3241 gacatctacg atttccaggc caccggcaag tgggccttcg acggcaagct cgaagacatc
     3301 acgagcgtga tcgacccgat ccgcgcgcgc ttcgcgccga acacgatcga gaccacgttc
     3361 ctctacaacg acaagacgaa gagccgcgcc tactacgcgt tcccgatcaa gcagcagacc
     3421 atgcacatcg agtactggct cgacatgctg accgatgccg gattcaagga gtccgacatc
     3481 ccgacgacct ggaaggagta ctggagcttc tggtgcgaca aggtgcagcc cgcctaccgc
     3541 cagaag
//
LOCUS       DATMJB010000473         2487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_832798, whole genome shotgun sequence.
ACCESSION   DATMJB010000473 DATMJB010000000
VERSION     DATMJB010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832798"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VNU48_10085"
     CDS             complement(<1..415)
                     /locus_tag="VNU48_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HWI11667.1"
                     /translation="MRGLLQCGAGAGRHTGAAQQILLKRSPLLQGSQAGNAERNCKRG
                     IRPTYWSLRVSNDFLFTSESVSEGHPDKVADQVSDAILDAIFKQDPLSRVAAETLTNT
                     GLVVLAGEITTNAHVDYIQVARDTIKRIGYDNTEYG"
     gene            466..1341
                     /locus_tag="VNU48_10090"
     CDS             466..1341
                     /locus_tag="VNU48_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWI11668.1"
                     /translation="MLSLFRWLARRPLWFLHALGAALGWLTYWTSPTYRRRFAANARQ
                     AGFTPAQARPAIAAAGRMVAELPFLWMRPAGEPIRPVVTWDGADLVAAAHAQGRGVLF
                     LTPHMGSFEVTAQAYAERFAATLAPATVLFRPARKAALRRLMGGSRARPGLATAPATL
                     AGVRQMIRALRRGEAVGLLPDQVPPEGMGVWVPFFGRPAYTMTLAARLIQQTGAVPLL
                     VWGERLAHGAGYRVRVSALGEDLPAADPTQAESAAVINRAMERLIRQCPGQYLWGYNR
                     YKTPRAADPARIDDP"
     gene            1434..2309
                     /locus_tag="VNU48_10095"
     CDS             1434..2309
                     /locus_tag="VNU48_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis acyltransferase"
                     /protein_id="HWI11669.1"
                     /translation="MGARFVLGLLWLLHWLPLGVQAGLGRGLGRLLYALAGARRRIAL
                     RNIALCFPEQDAAQHERLAREHFQWLGRSILERGLLWYASPARLRRLIHVEGDVQLAE
                     RSDRPVMWLVPHFMALDVAGVAVLLFQKRKGISIYQEQSDAVMDAAIRRGRLRLGNAE
                     IFPRDDSGKALFRAIRRGDAFFNLPDMDFGERDAAFVPFFGIPAATLLAPSRLARALN
                     MVVQPVLAETLPGGAGYRVRFMPAWTHFPTDDAQADTAAMNAWIESEIRALPAQYLWV
                     HKRFKTRPPGAAPLY"
     gene            complement(2272..>2487)
                     /locus_tag="VNU48_10100"
     CDS             complement(2272..>2487)
                     /locus_tag="VNU48_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11670.1"
                     /translation="AEFDARAGAVEHDEVIARALHLRELQPHARIMASAGGCAAFTSL
                     RDMSLRRNAPCALWAVSTAAPRRAAAS"
BASE COUNT          343 a          862 c          890 g          392 t
ORIGIN      
        1 tgccgtattc ggtgttgtcg tagccgatgc gcttgatcgt gtcgcgcgcg acctggatgt
       61 agtcgacgtg cgcgttcgtg gtgatctcgc cagccagcac aaccaggccg gtgttggtca
      121 gcgtctcggc ggcgacgcgc gacaggggat cttgcttgaa gatcgcatcg aggatcgcgt
      181 cggagacctg gtcggcgacc ttgtcggggt ggccttcgga gacggactct gaggtgaaaa
      241 ggaaatcgtt cgacactctt aaactccagt aggttgggcg gatgccgcgc ttgcagtttc
      301 gttctgcgtt gccggcctga gacccttgga ggagcggcga acgctttagc aggatttgtt
      361 gagccgcacc cgtgtgccgc ccggcgccgg ccccgcattg cagcaacccg cgcatcgccc
      421 gcaagtagtt caaattaact cggcgacgag ccgattataa agaagatgct gagcctgttt
      481 cgctggcttg cccgccggcc cctgtggttt ctgcacgcgc tcggcgccgc gttgggctgg
      541 ctgacgtact ggacctcgcc gacctatcgc cggcgcttcg ccgcgaacgc ccgccaggcc
      601 ggcttcacgc cggcccaggc ccgcccggcc atcgccgcgg ccgggcgcat ggtcgcggag
      661 ctgccgttcc tgtggatgcg gccggccggc gagccgatcc gcccggtcgt gacctgggac
      721 ggcgcggacc tcgtcgcagc cgcgcacgcg caaggccgcg gcgtgctgtt cctgaccccg
      781 cacatgggca gtttcgaagt cacggcgcag gcctatgcgg agcgcttcgc ggcgacgctg
      841 gccccggcga ccgtgctgtt ccggccggcg cgcaaggccg ccttgcggcg cctgatgggc
      901 ggctcgcgcg cccgcccggg cctggcgacc gcaccggcca cgctcgccgg cgtgcgccag
      961 atgatccgag cgctgcgccg cggcgaggcg gtcggcctgt tgcccgacca ggtgccgccc
     1021 gaaggcatgg gcgtgtgggt gccgttcttc ggccggccgg cctacacgat gacgctggcc
     1081 gcgcggctga tccagcagac cggcgcggtg ccgctgctgg tctggggcga gcggctcgcg
     1141 cacggcgccg gttaccgcgt gcgcgtcagc gcgctcggcg aagacctgcc cgcagcggac
     1201 cccactcagg cagaatccgc cgcggtcatc aaccgggcga tggagcggct gatccggcag
     1261 tgccccgggc agtacctctg gggctacaac cgctacaaga cgccgcgcgc ggccgacccg
     1321 gcaaggattg acgaccccta gacaccctcg gtgtcgtccc ccgcggggat cggccctctt
     1381 ggggcggccc agcgagggcc gagagccggg cgaacaaaga gaaggggtga acgttgggtg
     1441 cacgattcgt gctggggctg ttgtggctgc tgcactggct gccgctgggc gtgcaggccg
     1501 gtctcggccg cgggctgggc cggctgctct atgcactggc cggcgcgcgc cgccgcatcg
     1561 cgctgcgcaa catcgcgctg tgctttcccg agcaggacgc cgcgcaacac gagcgcctcg
     1621 cgcgcgagca cttccagtgg ctggggcgca gcatcctcga acgcggcctg ctctggtacg
     1681 cgtcgccggc gcgcctgagg cgcctgatcc atgtcgaggg cgacgtgcaa ctcgccgagc
     1741 gcagcgaccg gccggtgatg tggctggtgc cgcatttcat ggcactcgac gtcgccggcg
     1801 tcgcggtgct gctgttccag aagcgcaagg gcatctcgat ctatcaggag cagagcgatg
     1861 cggtgatgga cgccgcgatc cgccgcgggc ggctgcgtct gggcaatgcc gagatcttcc
     1921 cgcgcgacga ctcgggcaag gcgctgttcc gcgcgatccg ccgtggcgat gcattcttca
     1981 acctgcccga catggacttc ggcgagcgcg acgcggcctt cgtgcccttc ttcggcatac
     2041 ccgctgcgac gctgctggcg ccgtcgcggc tggcgcgcgc actgaacatg gtcgtgcagc
     2101 cggtgctcgc cgagaccctg cccggcggcg ccggctaccg cgtgcgcttc atgccggcct
     2161 ggacgcactt cccgaccgac gacgcgcagg ccgacacggc ggcgatgaac gcctggatcg
     2221 agtccgagat ccgcgcgctg ccggcgcagt acctgtgggt gcacaagcgc ttcaagacgc
     2281 ggccgcccgg cgcggcgccg ctgtactgac ggcccacaac gcacacggtg cgttgcggcg
     2341 caggctcatg tcgcgcagcg acgtgaaggc ggcgcagccg ccggccgaag ccataatccg
     2401 cgcatgcggc tgaagttcac gaagatgcag ggcgcgggca atgacttcgt cgtgctcgac
     2461 ggcacccgca cgcgcgtcga actcagc
//
LOCUS       DATMJB010000474         6539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_196451, whole genome shotgun sequence.
ACCESSION   DATMJB010000474 DATMJB010000000
VERSION     DATMJB010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196451"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VNU48_10105"
     CDS             complement(<1..1225)
                     /locus_tag="VNU48_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HWI11671.1"
                     /translation="MLNVRDLKVALDADAGLVKAIDGLTLAIQRGETFALVGESGCGK
                     SMTALALMRLLPDNGSVTNGQVLLEGEDVLGLPESGMRAVRGGRIGMIFQEPSTSLNP
                     VMRVGEQIVEAIQAHTALRGAAARAKAIEWLGKVGIPEPERRIDEYPFRLSGGQKQRV
                     MIAMTLAAEPDFLIADEPTTALDVTIQAQILDLLRRLQKEQGMGLLLITHDLAVVAGM
                     AQHVALMYAGQIIEVASADAFFAAPKHPYARLLMHALPDSAKRGDTLAAIPGMVPPLW
                     QSFDGCRFAPRCDRAFDACTGTRPELIASGAGAVRCLLYRDGAVPAAPAASGADSALC
                     FAAPPPKLPSAEPLLDVRHLSVRFPVRRGLLQRAAGAFVAVDDVSFTIAPGQTLALVG
                     ESGCGKTTTGKAVVQL"
     gene            complement(1236..2675)
                     /locus_tag="VNU48_10110"
     CDS             complement(1236..2675)
                     /locus_tag="VNU48_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI11672.1"
                     /translation="MPKIVILWSDVVVFATLVLLVLYGVRIRGSANLRATWLKVLRDR
                     AAICSAVVLVLFFAVAVLDSFHFRRALADAPSAGAQQRQAYGTRTESLLDVLLARQLE
                     GRETSYSAPLAIRGFTKDTVELDGKIARTYPRLAHGGERLKDESQWLPDLLQRAATGL
                     LLGVLVALLAALGIAFAVARVHREPLGTAWRALLRNQTELPWRAVVVTVLALCALFGP
                     LLALAQHYHVFGTDRTGNDVFYQALKSVRTAFVIGTLSTIATLPLAVGLGLVSGYLRG
                     WVDEVVQYLYTLLSSIPNVLLIAACVLMMQVFIDTHPDLFETGAERADVRLFMLCVIL
                     GLTGWATLCRLLRAETMKLSQLEYVQAATAFGVRSARIMVRHIFPNVVHLIIITTVLD
                     FSALILYEAVLSYVGVGVDPAMNSFGGMINLARTEMSRDPLVWWSFAAAFGFMVTLVL
                     AANLFADGVRDAFDPRSRSFRPKLRRSVA"
     gene            complement(2691..3674)
                     /locus_tag="VNU48_10115"
     CDS             complement(2691..3674)
                     /locus_tag="VNU48_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI11673.1"
                     /translation="MLNYVIRRCFYGVLVLVGVNLVTFMLFFTVNTPDDMARLNIGGK
                     RVTHEQIQKWKVERGYDKPLYWNAAAQGTRQATDTVFWDRSVSLMALDFGRSDSSSAT
                     DIGHEIGHRMLISLQLAVPIFVLQIVVSVAFALLLVFFKHSPIDFWGVVTCVLMLSIS
                     ALFYVIVGQFLFSRVFHLVPISGFESGWDAARFLILPVMLSLLSRLGSEGRLYRAMFL
                     EELGRDYVRTARAKGLSEPKVLFGHVLRNALIPILTSAGSYLPYVFLGSLVFESFFGI
                     PGLGAYVIAAITGQDFAIVRSMVFIGSLIYIASYIVIDVAYTWVDPRVRLA"
     gene            complement(3677..5956)
                     /locus_tag="VNU48_10120"
     CDS             complement(3677..5956)
                     /locus_tag="VNU48_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI11674.1"
                     /translation="MTMFTIMQGMAEARARGWPVLVALGGAALLAGCDNSPNPLGSES
                     TNTLFVAFQERSPRHLDPTASYALDEVKFTFAIYEPPYAYHYLKRPYEVVPKSALDIA
                     TPQYLDKDGKPLPADAPGELIAESDYDIHIKPGILFAPHPAFAKDATGAYLYHALTAA
                     QTRDKHTPLDFAQTGTRELVADDFVYALKRHATPRIEAPIYGIFSEYVIGLKDYGALI
                     RAEDKKLRQGTDPAALDKPFLDFRQYPLAGAFALDKYTYRIRIKGKYPQWKYWMTLTF
                     TAPVPWEADKFYAQPGMAKNGLKLDVWPVGTGPFMAVEYDADRRHVLVRNPNYHGATY
                     PCEGAPGDKEAGLLADCGKPLPFLDKLVFNTEKDKTPLRSKFTQGFLDVPEIERNDWG
                     PDFLADANDSDEVRKRFEERGMRFPTSIEPQNWYLGFNWLDPVVGKGDTPEQQEKNRK
                     LRYALSIAIDWEEGYGRVFTQKAGEAAMGPLPLGVFGSHHGSKDDINPVTHKVVDGKV
                     VRRSIAEAKQLLAEAGYPDGRDATTGKPLVLNYDYQRVPTPEFRAELDWMVKQFAKLG
                     VQLEIRATDYNQFQEKMLKGKQQIFWWGWFADYPDAENFLFLFYGPNSKAKAEGENTA
                     NYENPEYDRLYKQLALLDDGPEKQKLIDRMVDILRRDAPWSFGYFPYSAGAYQRWVGD
                     AKYGLFTNDRALYYKVDAPLRTRLQAEWNKPHSWPLVLIALAALLLAWIARRGFRARE
                     RMTALAPPVVPTADAKATT"
     gene            complement(5922..>6539)
                     /locus_tag="VNU48_10125"
     CDS             complement(5922..>6539)
                     /locus_tag="VNU48_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HWI11675.1"
                     /translation="GAKPVTWAGPLACDDFGRAMDAGGVDEVTRDCFLEAMSGALEAV
                     GFRTDAGFDGLEARGTLWGGNLCVVNSMLGTRYFPRIKGGVLFLEDVNEHPYRVERNL
                     LQLQQAGVLDAQKAVLLGEFSAWRKAPHDGGYSLKTVVQHLRALTKTPILTGLPFGHV
                     PTKVSLPVGARVTLLVQGRDALIGWDHRHAGGAHHDHVHDHAGHG"
BASE COUNT         1053 a         2247 c         2236 g         1003 t
ORIGIN      
        1 tcagctgtac cacggccttg ccggtggtcg tcttgccgca gccggactcg cccaccagcg
       61 cgagcgtctg gcccggcgcg atcgtgaacg acacgtcgtc gaccgcgacg aaggcgcccg
      121 ccgcccgctg cagcagcccg cggcggaccg ggaagcgcac gctcaggtgg cgcacgtcga
      181 gcagcggctc ggccgacggc agcttcggcg gcggcgcggc aaagcacagt gccgaatcgg
      241 cgccgctggc cgccggcgcc gcgggcaccg cgccgtcgcg atacagcaga cagcgcaccg
      301 cgcccgcgcc gctcgcgatc agttcggggc gggtgccggt gcacgcgtcg aacgcgcggt
      361 cgcagcgcgg cgcgaagcgg cagccgtcga acgactgcca cagcggcggc accatgccgg
      421 ggatcgcggc cagcgtgtcg ccgcgcttgg cgctgtcggg cagcgcgtgc atcagcaggc
      481 gcgcgtaagg gtgcttgggc gcggcgaaga acgcgtcggc gctcgccacc tcgatgatct
      541 ggcccgcgta catcagcgcg acgtgctggg ccatgccggc caccaccgcg aggtcgtgcg
      601 tgatcagcag caggcccatg ccctgctcct tctgcaggcg tctgagcagg tccaggatct
      661 gggcctggat cgtcacgtcg agcgcggtcg tgggttcgtc ggcgatcagg aagtcgggct
      721 cggcggccag cgtcatcgcg atcatcacgc gctgcttctg gccgcccgac aggcggaacg
      781 ggtactcgtc gatgcggcgc tcgggctcgg ggatgccgac cttgcccagc cactcgatcg
      841 ccttcgcacg cgccgcggcg ccgcgcagcg cggtgtgtgc ctggatcgcc tcgacgatct
      901 gctcgcccac gcgcatcacc gggttcaggc tcgtcgacgg ctcctgaaag atcatgccga
      961 tgcgcccgcc gcgcaccgcg cgcatgcccg actcgggcag ccccagaacg tcttcgccct
     1021 cgagcagcac ctggccgttc gtgacgctgc cgttgtcggg caacaggcgc atcagcgcga
     1081 gcgccgtcat gctcttgccg cagcccgatt cgccgaccag cgcgaaggtt tcgccacgct
     1141 ggatcgccag ggtcaggccg tcgatcgcct tcaccagccc ggcgtcggcg tcgagcgcca
     1201 ccttcaggtc gcggacgttc agcacgcgcg cgccttcatg cgacgctgcg ccgcagcttc
     1261 ggccggaagc tgcgcgagcg cgggtcgaac gcatcgcgca cgccgtcggc gaacaggttc
     1321 gccgccagca ccagcgtcac catgaagccg aacgccgcgg cgaacgacca ccacaccagc
     1381 gggtcgcggc tcatctcggt gcgtgccagg ttgatcatgc cgccgaagct gttcatagcc
     1441 gggtcgacgc cgacgcccac atacgacagc accgcctcgt acaggatcag cgccgagaag
     1501 tcgagcaccg tggtgatgat gatcaggtgc accacgttcg ggaagatgtg gcgcaccatg
     1561 atgcgcgccg agcgcacgcc gaacgcggtg gcggcctgca cgtattcgag ctgcgacagc
     1621 ttcatcgtct cggcgcgcag cagtcggcac agcgtggccc agccggtcag cccgaggatc
     1681 acgcacagca tgaacagacg cacgtcggcg cgctcggcgc cggtctcgaa caggtcgggg
     1741 tgggtgtcga tgaagacctg catcatcagc acgcaggcgg cgatcagcag cacgttcggg
     1801 atcgacgaca gcagcgtgta caggtactgc acgacctcgt cgacccagcc gcgcaggtag
     1861 cccgagacca gccccaagcc gaccgccagc ggcagcgtcg cgatcgtcga cagcgtgccg
     1921 atcacgaacg cggtgcgcac gctcttcagc gcctggtaga acacgtcgtt gccggtgcgg
     1981 tcggtgccga acacgtggta gtgctgcgcc agcgccagca gcggcccgaa cagcgcgcac
     2041 aacgccagca ccgtcacgac gacggcacgc cacggcagct cggtctggtt gcgcagcagc
     2101 gcgcgccagg ccgtgcccag cggctcgcgg tgcacgcgcg cgaccgcgaa cgcgatgccc
     2161 agcgcggcga gcagcgccac cagcacgccc agcagcagcc cggtcgcggc gcgttgcagc
     2221 aggtcgggca gccactgcga ttcgtccttc agccgctcgc cgccatgggc caggcgcggg
     2281 taggtgcgcg ctatcttgcc gtcgagctcg accgtgtcct tcgtgaagcc gcggatcgcc
     2341 agcggcgccg agtacgaggt ctcgcgtccc tcgagctggc gcgccagcag cacgtcgagc
     2401 agcgactcgg tgcgcgtgcc gtaggcctgg cgctgctgcg cgccggcgct gggcgcatcg
     2461 gccagcgcgc gccgaaagtg gaagctgtcg agcaccgcga ccgcgaagaa cagcaccagc
     2521 accacggccg agcagatcgc ggcgcggtcg cgcagcacct tcagccaggt cgcgcgcagg
     2581 ttcgcactgc cgcggatgcg cacgccgtac agcaccagca agaccagcgt ggcgaacacg
     2641 accacgtccg accagaggat gacgatcttg ggcacggcgc gaggtctcga tcaggccagg
     2701 cggacgcgcg ggtccaccca ggtgtaggcc acgtcgatga cgatgtagct ggcgatgtag
     2761 atcagcgaac cgatgaacac catcgagcgc acgatcgcga agtcctggcc cgtgatcgcc
     2821 gcgatcacgt acgcgcccag ccccgggatg ccgaagaagc tctcgaacac caggctgccg
     2881 aggaagacgt acggcaggta gctgccggcg ctggtcagga tcgggatcag cgcgttgcgc
     2941 agcacgtggc cgaacagcac cttgggctcg ctcagccctt tggcgcgcgc ggtgcgcacg
     3001 tagtcgcggc cgagttcctc gaggaacatc gcgcggtaca gccgcccttc gctgcccagg
     3061 cgcgacagca gcgacagcat caccggcagg atcaggaagc gcgccgcatc ccagcccgat
     3121 tcgaagcccg agatcggcac cagatggaac acgcgcgaga acaggaactg gccgacgatg
     3181 acgtagaaca gcgcgctgat cgaaagcatc agcacgcagg tcaccacgcc ccagaaatcg
     3241 atcggcgaat gcttgaagaa caccagcagc agcgcgaacg cgacgctgac gacgatctgc
     3301 agcacgaaga tcggcaccgc cagttgcagg ctgatcagca tgcggtggcc gatctcgtgg
     3361 ccgatgtcgg tcgcgctgct cgaatcggag cgcccgaagt ccagcgccat cagcgagacc
     3421 gagcggtccc agaacaccgt gtcggtggcc tggcgcgtgc cctgcgccgc ggcgttccag
     3481 tacagcggct tgtcgtagcc gcgctcgacc ttccatttct ggatctgctc gtgcgtgacg
     3541 cgcttgccgc cgatgttcag acgcgccatg tcgtccggcg tgttgacggt gaagaacagc
     3601 atgaaggtga cgaggttgac cccgaccagc accagcacgc cgtagaagca gcgtcggatg
     3661 acgtagttga gcatcgtcag gtcgtcgctt tggcgtcggc cgtgggcacg accggcggcg
     3721 ccagcgccgt catgcgctcg cgcgcccgga agccgcggcg cgcgatccac gccagcagca
     3781 atgccgcgag cgcgatcaac accagcggcc acgaatgggg cttgttccat tcggcctgaa
     3841 ggcgcgtacg cagcggcgcg tcgaccttgt agtacagcgc gcggtcgttc gtgaacaggc
     3901 cgtacttcgc gtcgccgacc cagcgctgat aggcgccggc cgaatacggg aaatagccga
     3961 acgaccacgg cgcatcgcgg cgcaggatgt cgaccatcct gtcgatcagc ttctgctttt
     4021 ccggcccgtc gtcgagcagc gcgagctgct tgtacaggcg gtcgtactcc gggttctcgt
     4081 agttcgcggt gttctcgccc tcggccttgg ccttcgagtt cgggccgtag aacaggaaca
     4141 ggaaattctc ggcgtcgggg tagtcggcga accagcccca ccagaagatc tgctgcttgc
     4201 ccttgagcat cttttcctgg aactggttgt agtcggtggc gcggatctcg agctgcacgc
     4261 cgagcttggc gaactgcttg accatccagt cgagttcggc gcggaactcg ggcgtgggca
     4321 cgcgctgata gtcgtagttc agcaccagcg gcttgccggt cgtggcgtcg cgtccgtcgg
     4381 ggtagccggc ctcggccagc agctgcttcg cctcggcgat cgaacggcgc acgaccttgc
     4441 cgtcgacgac cttgtgcgtg accgggttga tgtcgtcctt gctgccatgg tgcgagccga
     4501 acacgcccag cggcagcggg cccatcgccg cctcgccggc cttttgcgtg aagacgcggc
     4561 cgtagccctc ttcccagtcg atcgcgatgc tcagcgcgta gcgcagcttg cggttcttct
     4621 cctgctgctc gggcgtgtcg cccttgccga cgaccgggtc cagccagttg aagcccaggt
     4681 accagttctg cggctcgatg ctcgtcggga agcgcatgcc gcgctcctcg aagcgcttgc
     4741 gcacctcgtc ggaatcgttg gcgtcggcga gaaaatccgg gccccagtcg ttgcgctcga
     4801 tctcgggcac gtccagaaaa ccctgcgtga acttggagcg cagcggcgtc ttgtccttct
     4861 cggtgttgaa caccagcttg tcgaggaagg gcagcggctt gccgcagtcg gccagcaggc
     4921 ccgcctcctt gtcgccgggt gcgccctcgc acgggtaggt ggcgccgtgg tagttcgggt
     4981 tgcgcaccag cacgtggcgg cgatcggcgt cgtactcgac cgccatgaac gggccggtgc
     5041 cgaccggcca gacgtccagt ttcaggccgt tcttcgccat gccgggctgc gcatagaact
     5101 tgtcggcttc ccacggtacc ggcgcggtga aggtcagcgt catccagtac ttccactgcg
     5161 ggtacttgcc cttgatgcgg atgcgatagg tgtatttgtc gagcgcgaac gcaccggcca
     5221 gcgggtactg gcggaagtcg aggaagggct tgtccagcgc cgccgggtcc gtcccctggc
     5281 gcagcttctt gtcttcagcg cggatcagcg cgccgtagtc cttcagaccg atcacgtact
     5341 cggagaagat gccgtagatc ggcgcctcga tgcgcggtgt cgcgtggcgc ttgagcgcat
     5401 agacgaagtc gtcggcgacg agctcgcgcg tgccggtctg cgcgaagtcc agcggcgtgt
     5461 gcttgtcgcg cgtctgcgcg gctgtcaacg cgtggtacag gtacgcgccg gtcgcatcct
     5521 tcgcgaacgc cgggtgcggc gcgaacagga tgccgggctt gatgtggatg tcgtagtcgc
     5581 tctcggcgat cagctcgccg ggcgcgtcgg cgggcagcgg cttgccgtcc ttgtcgaggt
     5641 attgcggcgt ggcaatgtcg agcgccgact tcggcaccac ctcgtacggc cgcttcaggt
     5701 agtggtatgc atagggcggc tcatagatcg cgaacgtgaa cttcacctcg tcgagcgcgt
     5761 acgaggcggt cgggtcgagg tgccgcggcg agcgttcctg gaacgcgacg aacaaggtgt
     5821 tggtcgattc gctgccgagc gggttcgggc tgttgtcgca gccggcgaga agggccgcgc
     5881 cgccgagcgc gacgagcacc ggccatccgc gcgcgcgcgc ctcagccatg ccctgcatga
     5941 tcgtgaacat ggtcatggtg cgcgccgccg gcgtgccggt gatcccagcc gatcagcgcg
     6001 tcgcgcccct gcaccagcag cgtcacgcgc gcgcccaccg gcaggctcac cttggtcggc
     6061 acatggccga acggcagccc ggtcaggatc ggcgtcttcg tcagcgcgcg caggtgctgc
     6121 accacggtct tcagcgagta gccgccgtcg tgcggcgcct tgcgccaggc gctgaactca
     6181 ccgagcagaa ccgccttctg cgcatcgagc acgccggcct gttgcagctg cagcaggttg
     6241 cgctcgaccc ggtaggggtg ctcgttgacg tcctcgagga acagcacgcc gcccttgatg
     6301 cgcgggaagt agcgcgtgcc cagcatcgag ttgacgacac acaggttgcc gccccacagc
     6361 gtgccgcgtg cctccagccc atcgaagccg gcgtcggtgc gaaagcccac cgcttcgagc
     6421 gcgccgctca tcgcctcgag gaagcaatcg cgcgtgacct cgtcgacgcc gccggcatcc
     6481 atcgcccggc cgaagtcgtc gcaggccagc ggcccggccc aggtgacggg cttcgcgcc
//
LOCUS       DATMJB010000475         5149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_680603, whole genome shotgun sequence.
ACCESSION   DATMJB010000475 DATMJB010000000
VERSION     DATMJB010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680603"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..625
                     /locus_tag="VNU48_10130"
     CDS             68..625
                     /locus_tag="VNU48_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWI11676.1"
                     /translation="MKFVKTLLAAAALVASVGANAQSFTTGVGPGSYAAPSPSDVSPP
                     GSFSIQSTSVPNLYLAPLGDSSTFLVVNPGFTATIDLHGVTSFSFLWGSPDPSNAVSF
                     NGGAFMTGTAILGALANSSNANTQWITFTADAASGPLNTMSIKTGQIAFEMAVAAPVP
                     EPETYALMLAGLGAMAFVARRRKSS"
     gene            complement(705..1388)
                     /locus_tag="VNU48_10135"
     CDS             complement(705..1388)
                     /locus_tag="VNU48_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWI11677.1"
                     /translation="MQLYIGNKNYSSWSMRAWLLMTQAGIPFEEVPLRLSSEAASPFK
                     RALASITPAGRVPVLVDDGFAVWDTLAIAEYLAEKFADKRLWPVDTRRRARARSLCAE
                     MHGGFMALRNHCGMNIEADLAEVGARLLRDEPGVAGDLARIVEMWQQALGASGGPFLF
                     GDFSIADAYFAPVGARITTYALPVPADIGAYITRIFALPATRAWCEAARGEHDFVVED
                     EPYRTRPPG"
     gene            complement(1502..3562)
                     /locus_tag="VNU48_10140"
     CDS             complement(1502..3562)
                     /locus_tag="VNU48_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWI11678.1"
                     /translation="MTKTVTPKRFPDPVYGPTRRRGGALLVALILVAACAGIAPPVHA
                     QDGDALIVDARDALRKSDRTRLAALRATALASKLPLAMWPDYWELTNRIGEVQQAEVD
                     DFSARWPGTYVEDRFRNDWLLELGKRRDWVNFATEYPRFRMNDDREVSCYALLVEQRS
                     GKDVRDAALAAWLAQRDADDGCTLLAATLTDAKQFGPVEAWRKARLAIDAGRLRAARQ
                     AAALVSVSAASAVGELADNPARYLARQASTANRNAAELATLALMRMASNDPDAAAGQL
                     DSRWDRSLPADLAAWAWGAVGKQAAIKLQPGAADYFQRAAQRAAKANHELDWPDDTLA
                     WKARAALRADNGKARWQPVMQAINAMGAGEQREPVWVYWKARALAALAADSQDGDSMR
                     NLAREQMTSIAGQLNFYAALAAEQLGQPVWLPPRPAPLTAVEREAAARHPGLTRGLQL
                     IALGLRSEGVREWNYSIRGMDDRELLAAAQLACDREVWDRCINTSDRTRGEIDMEQRF
                     PMPFRKDVVAKAAEIGLDPAYVYGLIRQESRFIADARSSVGASGLMQLMPSTARWTAK
                     KIGLPYSHDLIADRDTNLRLGTSYLKLVLDDFGGSQALAAAAYNAGPGRPRKWRDGPV
                     LEPAIWAENVPFPETRDYVKKVLSNATYYAAQLGALAPSLKERLGRSIAPAGQANPLD
                     KELP"
     gene            3617..4153
                     /locus_tag="VNU48_10145"
     CDS             3617..4153
                     /locus_tag="VNU48_10145"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HWI11679.1"
                     /translation="MRKSLLEQRECFVAGATGSDVDAALARHLKAVISALEPACLGLY
                     WVHRAEFNAAAALTADPAFAKVPLALPFAQRAPVRLHYRAWDGRAPTTVDDCGIPASN
                     GAPVLPDVVLVPCVGYTDAGHRLGYGGGYFDRWLAQHPDVTAVGVAWSTGRLDDAVFA
                     AQPHDQPLAVIVTERGVV"
     gene            complement(4167..5135)
                     /locus_tag="VNU48_10150"
     CDS             complement(4167..5135)
                     /locus_tag="VNU48_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWI11680.1"
                     /translation="MSTGSRRPLSLGNLRAFEAVARRLSFSEAAEELFLTQSAISRQI
                     KSLEEELGAALFVRGTRHVEMTQSGQVLLRAVEAGLPKIDASVRQIRAARSRKRVSVT
                     TFASFGSLWLLPRIEAFQRRHPDIDIRVSAADAIADLDDPELDLALRYCSPAQAPDGA
                     IRMFGEVLTPVVSRSFAERIRRGEAPPLSRPADLAQHTLAEEDDTKASTEYLSWRRWL
                     SLQGQPALQPKRWLYLNFTYQQVQAALAGHGVALARIALVFEALQRGELVEPFGAEGR
                     MDSPYLYWLVVAPGSRARPEVREFGDWVLTQAADTRRAVGEVEPST"
BASE COUNT          717 a         1834 c         1808 g          790 t
ORIGIN      
        1 acccaacttg tgcggtctcc ggtccaaccc ggttgcacgg ccccccaacc catcgaaaag
       61 gtccatcatg aaatttgtaa agacactgct ggccgctgcg gcgctggtcg cgtcggtcgg
      121 cgcgaacgcg cagagcttca cgaccggcgt cggcccgggc agctacgcgg ctccgtcgcc
      181 gtcggacgtg agcccgccgg gcagtttctc gatccagagc accagcgtcc ccaacctcta
      241 cctggcaccg ctgggcgact cgagcacgtt cctggtcgtc aacccgggct ttacggcgac
      301 gatcgacctg cacggcgtta cgtcgttcag cttcctgtgg ggctcgccgg atccgagcaa
      361 cgctgtttcc ttcaatggcg gcgccttcat gaccgggacc gcgatcctcg gtgcgctggc
      421 caacagctcg aacgccaaca cgcagtggat cacgttcacc gctgatgcgg catcgggccc
      481 gttgaacacg atgagcatca agaccggcca gattgccttc gaaatggcgg tggcggcacc
      541 ggtcccggaa cccgagacct acgcgctgat gctcgcaggc ctgggcgcga tggccttcgt
      601 ggcgcggcgc cgcaagagca gctgaccgga cgggcggccc tcgaatgggg ccgtcagggg
      661 ccggagcgaa ggcggacctc gagtccgcct ttttttgtcc cgattcagcc gggcgggcgc
      721 gtccggtacg gctcgtcctc gacgacgaag tcgtgctccc cgcgtgcggc ctcgcaccag
      781 gcccgcgtcg ccggcagcgc gaagattcgc gtgatgtacg cgccgatgtc ggcggggacc
      841 ggcagcgcat aggtcgtgat gcgcgcgccc accggtgcga aataggcgtc ggcaatgctg
      901 aaatcgccga acaggaacgg gccgccgctc gcgcccagcg cctgctgcca catctcgacg
      961 atgcgcgcca gatcgcccgc cacgccgggc tcgtcgcgca gcaaccgtgc cccgacctcg
     1021 gcgagatcgg cttcgatgtt catgccgcag tggttgcgca gcgccatgaa gccgccgtgc
     1081 atttcggcgc acagcgagcg cgcccgcgcg cgccggcgcg tgtcgactgg ccagagccgc
     1141 ttgtcggcga acttctcggc caggtactcg gcgatcgcca gcgtgtccca gaccgcgaag
     1201 ccgtcgtcga ccagcaccgg caccctgccg gcgggcgtga tgcttgcgag cgcgcgcttg
     1261 aatggcgaag cggcctcgga cgacagacgc agcgggactt cctcgaacgg gatgccggcc
     1321 tgcgtcatca gcagccacgc ccgcatcgac caggacgagt agttcttgtt gccgatgtag
     1381 agctgcatgc ggatcctcac gggtggaaca gcaggcgatg ctacccaggc gacgcgcggc
     1441 gcggcgtgac gctttcgcat ccgattgatg ccggcgccgc atcggcgcgg ccgtcaccgc
     1501 gtcagggcag ttccttgtcg agcgggttcg cctggccggc cggggcgatc gaccgcccga
     1561 ggcgttcctt cagcgagggc gccagcgcgc ccaactgcgc agcgtagtag gtcgcgttgc
     1621 tgagcacctt cttgacatag tcgcgggtct cgggaaacgg cacgttctcg gcccagatcg
     1681 cgggctccag caccggcccg tcgcgccatt tgcgcggccg gccgggcccg gcgttgtagg
     1741 ccgccgcggc gagcgcctgc gagccgccga aatcgtcgag cacgagcttg aggtagctgg
     1801 tgcccaggcg cagattggtg tcgcggtcgg cgatcaggtc gtggctgtag ggcaggccga
     1861 tcttcttcgc ggtccagcgc gcggtcgacg gcatcagctg catcaggccg gaggcgccca
     1921 cgctcgagcg cgcatcggcg atgaagcgcg actcctggcg gatcagcccg tacacgtagg
     1981 ccgggtcgag gccgatctct gcggccttcg cgaccacgtc cttgcggaac ggcatcggaa
     2041 agcgctgctc catgtcgatc tcgccgcgcg tgcgatcgct ggtgttgatg cagcggtccc
     2101 agacctcgcg gtcgcaggcg agttgtgcgg cggccagcaa ctcgcgatcg tccatgccgc
     2161 ggatcgaata gttccactcg cgcacgcctt cgctgcgcag cccgagcgcg atcagctgca
     2221 gcccgcgcgt caggcctggg tggcgcgccg ccgcctcgcg ctcgaccgcg gtcagcggcg
     2281 ccggtcgcgg cggcagccag accggctggc cgagttgctc ggcggcgagc gccgcgtaga
     2341 agttcagttg cccggcgatg ctcgtcatct gctcgcgtgc caggttgcgc atcgagtcgc
     2401 cgtcctgcga atcggccgcc agcgcggcca gtgcgcgggc cttccagtag acccacaccg
     2461 gctcgcgctg ctcgcccgcg cccatcgcgt tgatggcctg catcaccggc tgccagcgcg
     2521 ccttgccgtt gtcggcgcgc aaggcggcgc gggccttcca ggcgagcgtg tcgtcgggcc
     2581 agtcgagttc gtggttcgcc tttgccgcgc gttgcgcggc gcgctgaaag tagtcggcgg
     2641 ccccgggctg cagcttgatc gccgcctgct tgccgaccgc gccccaggcc caggcggcca
     2701 ggtcggccgg caggctgcga tcccagcggc tgtcgagctg gccggccgca gcatcggggt
     2761 cgttcgaggc catgcgcatc agcgccagcg tcgccagctc ggcggcgttg cggttcgccg
     2821 tcgaggcctg gcgcgcgagg tagcgcgccg ggttgtccgc gagttcgccg accgcgctgg
     2881 ccgcgctcac gctgacgagc gcagcggcct ggcgcgccgc gcgcagccgg ccggcgtcga
     2941 tcgcgaggcg cgccttgcgc caggcttcga cagggccgaa ctgcttcgcg tcggtcaggg
     3001 tcgcggcgag cagggtgcag ccgtcgtcgg catcgcgctg cgccagccag gcggcgagcg
     3061 cggcgtcgcg cacgtccttg ccggaccgtt gctcgaccag cagcgcatag caactcacct
     3121 cgcggtcgtc gttcatgcgg aagcgcgggt actcggtggc gaaattgacc cagtcgcgcc
     3181 gtttgccgag ctcgagcagc cagtcgttgc ggaagcggtc ctcgacatag gtgccgggcc
     3241 agcgcgcgct gaagtcgtcg acctcggcct gctgaacctc gccgatgcgg ttcgtcagct
     3301 cccagtagtc gggccacatc gccagcggca gcttgctcgc gagcgccgtg gcgcgcagcg
     3361 cggccagccg ggtgcgatcg ctcttgcgca gcgcgtcgcg ggcgtcgacg atgagcgcgt
     3421 cgccgtcctg cgcatgcacc ggcggcgcga tgccggcaca ggcggcgacc aggatcagcg
     3481 cgaccagcaa cgcgccgccg cggcgccgag tcggcccata tactggatcg ggaaatcgct
     3541 tcggggtcac cgtcttcgtc atggagtcat cttgagttcc gcatcgcctg gagccccgtc
     3601 ggggccgcga tcggcgttgc gcaaaagcct gttggagcag cgcgaatgct tcgttgccgg
     3661 cgcgacgggc agcgacgtcg atgccgcact ggcgcgtcat ctgaaagccg tgatatcggc
     3721 gctggagcct gcgtgcctcg gtctgtactg ggtgcaccgt gccgaattta acgcagccgc
     3781 cgcgctcacc gccgaccccg cgttcgcaaa ggtgccactc gcgttgccgt ttgcgcagcg
     3841 cgcaccggtg cggctgcact accgcgcgtg ggacggccgc gcccccacga ccgtcgacga
     3901 ctgcggcatc ccggccagca acggcgcgcc ggtgttgccc gacgtggtgc tcgtgccctg
     3961 cgtcggctac acggacgcag ggcatcggct cggctatggc ggcggctact tcgaccgctg
     4021 gctcgcgcag cacccggacg tgaccgcggt cggcgtcgcc tggagcaccg gcaggctcga
     4081 cgacgccgtg ttcgccgcgc agccgcacga ccagccgctc gccgtgatcg tcaccgagcg
     4141 cggcgtggtc tgagcacgct gcccgatcag gtgctcggct cgacctcgcc gacggcgcgg
     4201 cgggtatcgg ccgcctgcgt cagtacccag tcgccgaact ccctgacctc gggccgcgcg
     4261 cggctgcccg gcgcgaccac cagccagtac agatacgggc tgtccatgcg cccttcggcg
     4321 ccgaagggct cgaccagctc gccgcgctgc agcgcctcga acacgagcgc gatgcgcgcc
     4381 agtgcgacgc cgtggccggc cagcgccgcc tgcacctgct ggtaggtgaa gttcaggtac
     4441 agccagcgct tgggctgcag cgcgggctgg ccctgcaggc tcagccagcg gcgccagctc
     4501 aggtactcgg tactggcctt ggtgtcgtcc tcctcggcca gcgtgtgctg cgccagatcg
     4561 gcgggccgcg acagcggcgg cgcctcgccg cgccggatcc gctctgcaaa cgagcggctg
     4621 acgaccggcg tcagcacctc gccgaacatc cggatcgcgc cgtccggggc ctgtgccggg
     4681 ctgcagtagc gcagcgccag atcgagctcg gggtcgtcga gatcggcgat cgcgtcggct
     4741 gccgagacgc ggatgtcgat gtcgggatgc cggcgctgga aggcctcgat ccgcggcagc
     4801 agccacagcg aaccgaacga cgcgaaggtc gtgacactga cgcgcttgcg gctgcgcgcg
     4861 gcgcggatct ggcgcacgct ggcgtcgatc ttcggcagcc cggcctcgac cgcgcgcaac
     4921 aggacctggc cgctctgcgt catctcgacg tggcgcgtgc cgcgcacgaa cagcgccgcg
     4981 ccgagctcct cttccaggct cttgatctgc cggctgatcg ccgactgcgt caggaacagt
     5041 tcttcggccg cctcgctgaa gctgagccgc cgggccactg cctcgaacgc gcgcaggttg
     5101 cccagcgaga gggggcggcg ggaaccggta ctcatatcaa atccgcatc
//
LOCUS       DATMJB010000476         4175 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_466881, whole genome shotgun sequence.
ACCESSION   DATMJB010000476 DATMJB010000000
VERSION     DATMJB010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466881"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..547
                     /locus_tag="VNU48_10155"
     CDS             <1..547
                     /locus_tag="VNU48_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI11681.1"
                     /translation="GYHHVNEVWLDDVRVPVDNLVGEEGAGWTCAKFLLRNERTAGAM
                     VGTVSHALARLKAMPAPSTALRQRIGELDLRAFALEECAYQAMEAMLEGRENGGEASL
                     IKLRGTELYQDIAEGLVDALGPAGIAWDAEALHRADLPPLGPDDAGGIVKDHLYNRAA
                     TIFGGSSEVQRNILAKAVLGL"
     gene            544..1632
                     /locus_tag="VNU48_10160"
     CDS             544..1632
                     /locus_tag="VNU48_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007738864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWI11682.1"
                     /translation="MTTDTDTVEMLRDTMQRYAAERYGFAQRRAFLAEGCSDRAWRDY
                     AEFGWLALRLPEDDGGLAADAGAIGAVMEVVGARLLLEPVLASAVLGTGLVLACASAA
                     QRAELLPALADGTLKLAFACDDDPTAGPACELRGERLHGGKLNVLHGDVAGRLIVSAR
                     DADAAGGWALCLVDPAASGVERRPYRLIDGRGAAALRFQAARVERLGAAEATDAAEAI
                     ARARDEASAALCAEALGVVRSLVAATCDYLKLRKQFGRTIGSNQALQHRMVELFLLQQ
                     EVQALTRAAQRGLDAEPAQRARAVSGARAYIATAARRVANEAVQMHGGVGITEELDVS
                     HHFRRVMVINALFGNRDEHFARYVEAAR"
     gene            1629..3257
                     /locus_tag="VNU48_10165"
     CDS             1629..3257
                     /locus_tag="VNU48_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI11683.1"
                     /translation="MNRRSTPQSAAFVERYRHAGVWLDKTVAEFAAEAAEREPDRITH
                     VFEGQACRIADLLSEAQALAAALQSRGLGSGDVIGFQLPNWREALVIDLAAALLGLVV
                     APIVPIYRDAEVAHMLADSGARAAFFPTTYRGFDHAAMMRRLAPTLPGLRLCCSVRGA
                     VPAADSYEALIAEQRPWRGAPPADANAVKLLLYTSGTTGRPKAVLHSHNSGPVTLRKA
                     FEHWGQQPGDTVLMASPVTHITGFSYGMELPMICGTRTVFMERWDAAEAVALIEREQV
                     TASMGATPFLHELLSEAARQGRRLPSLRLFACGGAEVPQALIRRCADVLQSCRAFRVY
                     GSSEAPLVTLGFVRDGTLDLAATTDGEVVHYDVRIVDDAGHELPFGQDGEICVRGPAM
                     ALGYADAAQSAESFDADGFFATGDLGRLTPERAIVVTGRKKDLINRGGEKISAKEVED
                     ILHRHPAVDEAAVVAMPHERLGETVCAYVVPRQGHDLDLAQVKQCMATSGVARQKHPE
                     RLVLVEGLPRTASGKVRKDQLRLDIRERLRAGTP"
     gene            3254..>4175
                     /locus_tag="VNU48_10170"
     CDS             3254..>4175
                     /locus_tag="VNU48_10170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HWI11684.1"
                     /translation="MNEALREVRIGRKTLTALDIVELELLPLHGGALPAFSAGAHIDV
                     EVRPGLLRQYSLCNDPSETSRYQIGVLRDPYSRGGSAAVHDALAVGQVIRIGTPRNRF
                     ALVPARRSLLFAGGIGITPLLCMAEQLARDGAAFEMHYCTRSPERTAFAARIAASAFA
                     DHVAVHHDDGPAVQRLDLLRALPAPDAQTHLYVCGPRGFIDWVCGAASAAGWAPAQIH
                     FEHFAGQEPGRAEDCAFEVRLAGSGRTIPVPPGRSVAAALHAHGIELPVSCEQGVCGT
                     CLTRVLAGEPDHRDSYLTDEERCRNDQFLPC"
BASE COUNT          598 a         1525 c         1482 g          570 t
ORIGIN      
        1 cggctatcac cacgtcaacg aggtctggct ggacgacgtg cgtgtgccgg tggacaacct
       61 cgtcggcgaa gaaggtgcgg gctggacctg cgccaagttc ctgctgcgca acgagcgaac
      121 cgccggcgcg atggtcggca cggtgagcca cgcgctggcc cggctgaagg cgatgcccgc
      181 gccaagcacg gcgctgcggc agcgcatcgg cgaactcgac ctgcgcgcct tcgcgctgga
      241 ggagtgcgcc taccaggcga tggaagcgat gctcgaaggc cgcgagaacg gcggcgaggc
      301 ttcgctgatc aagctgcgcg gcaccgagct gtaccaggac atcgcggaag gcctggtcga
      361 tgcgctcggg ccggccggca tcgcctggga cgcggaggcg ctgcaccgcg ccgacctgcc
      421 gccgctcggc cccgacgacg ccggcggcat cgtcaaggac cacctctaca accgcgccgc
      481 gaccatcttc ggcggctcgt ccgaggtgca gcgcaacatc cttgcgaagg cggtgctggg
      541 cctatgacaa cggacaccga caccgtcgag atgctgcgcg acacgatgca gcgctacgca
      601 gccgaacgct atggcttcgc gcagcgccgc gccttcctgg ccgagggctg cagcgaccgc
      661 gcctggcgcg actacgccga gttcggctgg ctcgcgctgc gcctgcccga ggacgacggc
      721 ggcctggccg ccgatgccgg cgcgatcggc gcagtgatgg aagtggtcgg cgcccggctg
      781 ctgctggagc ccgtgctcgc cagcgccgtg ctgggcaccg gactcgtgct ggcctgtgcc
      841 agcgccgcgc agcgcgccga actgctgccg gcgctggccg acggcacgct caagctcgcc
      901 ttcgcttgcg acgacgaccc gacggccggc ccggcctgcg aactgcgtgg cgagcgtctg
      961 cacggcggca agctcaatgt gctgcacggc gatgtggcgg gccgactgat cgtcagcgca
     1021 cgcgatgccg acgccgccgg cggctgggcg ctgtgcctgg tggacccggc cgccagcggc
     1081 gtcgagcgcc ggccttaccg gctgatcgat gggcgcggcg ccgcagcgct gcgcttccag
     1141 gccgcacggg tcgaacgact cggcgcggcc gaggccaccg acgcggccga ggccatcgcc
     1201 cgcgcccgcg acgaagcgtc cgccgcgctg tgcgccgagg ccttgggcgt cgtgcgcagc
     1261 ctggtcgccg caacctgcga ctacctgaag ctgcgcaagc agttcggccg caccatcggc
     1321 agcaaccagg ccctgcaaca tcgcatggtc gagctgttct tgctgcagca ggaagtgcag
     1381 gcactgacgc gcgccgcaca gcgcggcctg gatgccgagc ctgcgcagcg cgcccgcgcg
     1441 gtcagcggcg cccgcgccta catcgcgacg gcggcgcgac gcgtcgccaa cgaggcggtg
     1501 cagatgcatg gcggcgtcgg catcaccgag gagctggacg tcagccacca cttccgccgc
     1561 gtgatggtga tcaacgcgtt gttcggcaac cgcgacgagc acttcgctcg ctacgtcgag
     1621 gccgcgcgat gaaccgccgc tccacgccgc agtccgccgc attcgtcgag cgttaccggc
     1681 atgccggcgt ctggctggac aagaccgtgg ccgagttcgc cgccgaagcg gccgagcgcg
     1741 agcccgatcg catcacgcat gtcttcgagg gccaggcctg ccgcatcgcc gatctgctga
     1801 gcgaggcgca ggcgctggcc gccgcgctcc agtcccgcgg cctgggcagc ggcgacgtca
     1861 tcggctttca gctgccgaac tggcgcgagg cgctggtcat cgacctggcg gccgcgctgc
     1921 tgggcctggt cgtcgcgccc atcgtgccga tctaccgcga cgccgaggtg gcccacatgc
     1981 tggccgattc gggcgctcgc gcggcgttct tcccgaccac ctaccgcggc ttcgaccacg
     2041 ccgcgatgat gcggcgcctg gcgccgacac tgcccgggtt gcggctgtgc tgcagcgtgc
     2101 gcggcgccgt gcccgccgcc gacagctacg aagcgctgat cgccgaacaa cgaccctggc
     2161 gcggcgcgcc gccggccgat gcgaacgcgg tcaagctgct gctctacacc tcgggcacga
     2221 ccggccggcc gaaggcggtt ctgcactcgc acaacagcgg cccggtgacc ttgcgcaagg
     2281 ccttcgagca ctggggccag caacccggcg acacggtgct gatggcctcg ccggtgaccc
     2341 acatcaccgg cttcagctac ggcatggaac tgccaatgat ttgcggcacg cgcaccgtgt
     2401 tcatggagcg ctgggacgct gccgaagcgg tggcgctgat cgaacgtgag caggtcaccg
     2461 ccagcatggg cgcgacgccc ttcctgcacg agctgctgag cgaagcggcg cgccagggac
     2521 ggcggctgcc ttcgctgcgt ctgttcgcct gcggcggcgc cgaggtgccg caggcactga
     2581 tccggcgctg cgccgacgtg ctgcagagct gccgcgcctt ccgcgtctac ggctccagcg
     2641 aggcgccgct ggtcacgctg ggcttcgtgc gcgacggcac gctggacctg gccgccacga
     2701 ccgacggcga ggtggtgcac tacgacgtgc gcatcgtcga cgacgcgggc catgagctgc
     2761 ccttcggcca ggacggcgag atctgcgtgc gcggcccggc catggcgctg ggctatgccg
     2821 acgccgcgca gtcggccgag agcttcgatg ccgacggctt cttcgccacc ggcgacctgg
     2881 gccgcctgac acccgagcgc gccatcgtcg tcaccgggcg caagaaggac ctgatcaacc
     2941 gcggcggcga gaagatcagc gccaaggagg tcgaggacat cctgcaccgc catcccgcgg
     3001 tcgacgaggc ggcggtggtc gcgatgccac acgagcggct cggcgagacg gtgtgcgcct
     3061 atgtcgtgcc ccggcagggc cacgacttgg acctcgcgca ggtgaagcag tgcatggcga
     3121 cctccggcgt ggcacggcag aagcatcccg agcgcctggt gctggtcgag ggcctgccgc
     3181 gcaccgcgtc gggcaaggtg cgcaaggacc agctgcggct ggacatccgc gagcggctgc
     3241 gagccggcac gccatgaacg aagccctgcg tgaagtgcgc atcggccgca agaccctcac
     3301 ggccctggac atcgtggaac tcgagctgct gccgctgcac ggcggcgcgc tcccggcctt
     3361 cagcgccggc gcccacatcg acgtcgaagt gcggcccggg ctgctgcgcc agtactcgct
     3421 gtgcaatgac ccgtcggaga cgagccgcta ccagatcggc gtgctgcgcg atccttattc
     3481 gcgcggcggc tcggccgccg ttcacgacgc gctcgccgtg ggccaggtga tccgcatcgg
     3541 cacgccgcgc aaccgcttcg cgctggtgcc ggcgcggcgc agcctgctct tcgccggcgg
     3601 catcggcatc acgccgctgc tgtgcatggc cgaacaattg gcgcgcgacg gcgcggcctt
     3661 cgaaatgcac tactgcacgc gctcgccgga acgcaccgcg tttgcggcgc gcatcgcggc
     3721 ctcggccttc gccgaccatg tggctgtcca tcacgacgat ggcccggcgg tgcagcgact
     3781 cgatttgctg cgcgcgctgc cggcgcccga cgcgcagacc catctgtacg tgtgcggccc
     3841 gcgcggcttc atcgactggg tgtgcggcgc cgccagcgcg gcgggttggg caccggcgca
     3901 gatccacttc gaacacttcg cgggccagga gccgggccgc gccgaagact gcgcgttcga
     3961 ggtccggttg gccggcagcg gccggaccat ccccgtgcca cccggccgct cggtggccgc
     4021 cgcgctgcac gcgcacggca tcgagctgcc cgtctcgtgc gaacagggtg tgtgcggcac
     4081 ctgcctgaca cgcgtcctcg ccggcgagcc cgatcaccgc gacagctacc tcaccgatga
     4141 agaacgctgc cgcaacgacc agttcctgcc ctgct
//
LOCUS       DATMJB010000477         3377 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_374280, whole genome shotgun sequence.
ACCESSION   DATMJB010000477 DATMJB010000000
VERSION     DATMJB010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374280"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VNU48_10175"
     CDS             <1..252
                     /locus_tag="VNU48_10175"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829568.1"
                     /note="catalyzes the formation of 2-C-methyl-D-erythritol
                     4-phosphate from 1-deoxy-D-xylulose-5-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HWI11685.1"
                     /translation="LALAWETLRGPEGSSTVLNAANETAVAAFLAGTIRFDQIHSVIA
                     QALERVDVRSAGHDSLEALLEIDHRARRQAAQFVKGLAP"
     gene            249..1619
                     /gene="rseP"
                     /locus_tag="VNU48_10180"
     CDS             249..1619
                     /gene="rseP"
                     /locus_tag="VNU48_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HWI11686.1"
                     /translation="MIITVLAFILTLGVLITIHEYGHYRVAVACGVKVLRFSIGFGRV
                     LWRRQATPDSTEFVVCALPLGGYVKMLDEREAPVASDQLGGAFNRKPLLQRTAIVAAG
                     PAANLLLAVLLYAAAHWIGIDEAKAVMGPPVAASLADRAGLRSGDWAREWSNDGVEWH
                     ELRSMTDLRWEVMQAALRGAPLQLMVTDRAGHARRSVVLALDTLGSTEVDAKQMQRIG
                     LGAAFSEPVLGTVMVGGPADKAGLRAGDRVLSIDGTAVADGWSLRELIVASGRDGRAR
                     PMQWRVDRDGKTIELAVTPNVVQDGGKSIGRVELVPGQPPEMVTVRYGVFDGLSQAVS
                     QTWQMSTLTVKMLGKMVIGQASLKNLSGPLTIADYAGQSVRLGLAYYLGFLAVVSVGL
                     GVLNLLPLPVLDGGHLMYYLFEGVTGRPVSELWLDRLQRGGVAIMLMMMSLALYNDVA
                     RLLGLQ"
     gene            1630..>3377
                     /gene="bamA"
                     /locus_tag="VNU48_10185"
     CDS             1630..>3377
                     /gene="bamA"
                     /locus_tag="VNU48_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HWI11687.1"
                     /translation="MRFSSRLTSFRPSLISVALAAALQAGSAWAVEPFAVRDIRVEGL
                     QRSDAGTVFASLPFRIGDTYSDEKGAAALRALFATGLYKDVRIDIDGNVVVVIVEERP
                     VIANIDFVGLKEFDKDQLIKSLRDFGISDGQPFDKALADRAEQELKRQYLTRSLYGAE
                     VVTTVTPQERNRVNLSFTITEGSAAKISEIRITGNKAFSESTLTGLFDLNAGGWLNFY
                     TKADRYSRAKLDADLETLRAYYLNRGYLEFNVESTQVAISPDKQDITITVNVKEGQPY
                     TVSGVRLEGDYLGKEDDFKTLVQIKPGEPYRAETVTQTTRAMSDRFGTFGYAFAKVEA
                     RPEIDRANGTVALTITAEPARRVYIRRINVSGNTKTRDEVIRREFRQFESSWYDGRKI
                     KLSRDRVDRLGYFSEVSVDTNEVPGTADQVDLTIAVKEKATGNLQIGAGFSSADKLSL
                     SGSIKQENVFGSGNYLGLELNTSKYSRTLVLSTIDPYFTVDGISRYIDVFYRTQQPIN
                     SQGELYKLITPGASIRFGVPFSEFDTVFFGIGIEQTRIEGSNLPVNYRNYVTEFGNSS
                     SSIPLTIGWSRDSRDSA"
BASE COUNT          562 a         1141 c         1136 g          538 t
ORIGIN      
        1 ctcgcgctcg cgtgggagac gctgcgcggc cccgaaggga gctccacggt gctgaacgcg
       61 gccaacgaga cggcggtcgc cgcgtttctc gccggaacca tccgcttcga tcagattcac
      121 agcgtgatcg cgcaggccct cgaacgcgtc gacgttcgtt ccgccgggca cgactcgctc
      181 gaagccctgc tcgagatcga ccatcgcgcc cgccgccagg cggcgcagtt cgtgaagggc
      241 ctcgcgccat gatcatcacc gtgcttgcgt tcatcctcac gctgggcgtg ctgatcacga
      301 tccacgagta cggccactac cgcgtcgcgg tcgcctgcgg cgtcaaggtg ctgcgcttct
      361 cgatcggctt cgggcgcgtg ctgtggcggc gccaggccac cccggacagc accgagttcg
      421 tcgtctgcgc gctgccgctg ggcggctacg tgaagatgct cgacgaacgg gaagcacccg
      481 ttgcttccga tcaactcggt ggcgcgttca accgcaagcc gctgctgcag cgcaccgcga
      541 tcgtcgccgc cgggccggcc gccaacctgt tgctcgcggt gctgctgtac gccgccgcgc
      601 actggatcgg catcgacgag gcgaaggcgg tgatgggccc gccggtggcc gccagcctgg
      661 ccgatcgggc cgggctgcgc agcggcgact gggcgcgtga gtggtcgaac gacggcgtcg
      721 agtggcacga gctgcgctcg atgaccgacc tgcgctggga ggtcatgcag gccgccctgc
      781 gtggtgcgcc gctgcagctg atggtgaccg atcgcgccgg ccacgcacgg cgcagcgtcg
      841 tgctggcgct cgacacgctg ggcagcaccg aggtcgacgc caagcagatg cagcgcatcg
      901 ggctgggcgc tgcgttcagc gagccggtgc tgggcacggt catggtcggc gggccggccg
      961 acaaggccgg cctgcgcgcc ggcgaccgcg tgctgagcat cgacggcacg gcggtggccg
     1021 acggctggtc gctgcgcgag ctgatcgtcg cgagcggccg cgacggccgg gcgcggccca
     1081 tgcagtggcg tgtcgatcgc gacggcaaga cgatcgagct ggcggtgacg cccaatgtcg
     1141 tgcaggacgg cggcaagagc atcggccggg tcgaactcgt gcccggccag ccgcccgaga
     1201 tggtgacggt gcgctacggc gtgttcgacg gcctgtcgca ggcggtgtcg cagacctggc
     1261 agatgtcgac gctgaccgtg aagatgctgg gcaagatggt gatcggccag gcttcgctga
     1321 agaatctcag cgggccgctg acgatcgccg actacgcggg ccagtcggtg cgactcggtc
     1381 tggcgtacta cctgggcttt ctggcggtcg tcagcgtcgg cttgggtgtg ctcaacctgt
     1441 tgccgttgcc ggtcctcgat ggcgggcacc tgatgtatta tcttttcgag ggtgtgacgg
     1501 gacggccggt ctccgagttg tggcttgacc ggctgcagcg tggcggggtg gccatcatgc
     1561 tcatgatgat gtccctggct ctctacaacg atgtggcccg cctcctgggc ctgcaatgac
     1621 cccgaccgca tgcgcttctc ctcccgcttg acctcgtttc gcccctccct catttccgtc
     1681 gcgctggccg ctgccctgca ggccgggtcg gcttgggcgg tcgagccctt tgcggtcagg
     1741 gacatccgcg tcgagggcct gcagcgcagc gatgccggca cggtgttcgc gtcgctgccg
     1801 tttcgcatcg gcgacaccta cagcgacgag aagggcgccg ccgcgctgcg cgcgctgttc
     1861 gccaccggcc tgtacaagga cgtgcgcatc gacatcgacg gcaacgtcgt cgtcgtgatc
     1921 gtcgaggagc ggccggtcat cgccaacatc gacttcgtcg gcctgaagga gttcgacaag
     1981 gaccagttga tcaagtcgct gcgcgacttc ggcatcagcg acggccagcc cttcgacaag
     2041 gccctggccg accgcgccga gcaggagctc aagcgccagt acctgacgcg cagcctgtac
     2101 ggcgccgagg tcgtgaccac cgtgacgccg caggagcgca accgcgtgaa cctgagcttc
     2161 acgatcaccg agggctcggc cgcgaagatc agcgagatcc gcatcaccgg caacaaggca
     2221 ttctcggaaa gcacgctgac gggcctgttc gacctcaatg ccggcggctg gctcaatttc
     2281 tacaccaagg ccgaccgcta ctcgcgcgcc aagctcgacg ccgacctcga aacgctgcgc
     2341 gcctactacc tgaaccgcgg ctacctcgag ttcaacgtcg agtcgaccca ggtcgcgatc
     2401 tcgcccgaca agcaggacat cacgatcacc gtcaacgtga aagagggcca gccctacacg
     2461 gtgagcggcg tcaggctcga gggcgactac ctcggcaagg aagacgactt caagaccctg
     2521 gtgcagatca agccgggcga gccgtaccgc gccgagaccg tgacgcagac gacgcgcgcg
     2581 atgagcgatc gtttcggcac cttcggctat gcgttcgcga aggtcgaggc gcgccccgag
     2641 atcgaccgcg cgaacggcac cgtcgcgctg acgatcaccg ccgagccggc gcgccgcgtg
     2701 tacatccggc gcatcaacgt ctcgggcaac accaagacgc gcgacgaggt catccggcgc
     2761 gagtttcgcc agttcgaatc gagctggtac gacgggcgca agatcaagct gtcgcgcgac
     2821 cgggtcgacc ggctcggcta cttcagcgag gtcagcgtcg acaccaacga ggtgcccggc
     2881 accgccgacc aggtcgacct gacgatcgcg gtcaaggaga aggccaccgg caacctgcag
     2941 atcggcgccg gcttctcgtc agccgacaag ctgtcgctgt cgggctcgat caaacaggag
     3001 aacgtgttcg gctccggcaa ctacctgggc ctggaactga acaccagcaa gtacagccgc
     3061 acgctggtgc tcagcacgat cgacccgtac ttcacggtcg acggcatctc gcgctacatc
     3121 gacgtgttct atcgcacgca gcagccgatc aacagccagg gcgagttgta caagctcatc
     3181 acgccgggtg cgtcgatccg cttcggcgtg ccgttcagcg agttcgacac ggtgttcttc
     3241 ggtatcggca tcgagcaaac ccggatcgaa ggctcgaacc tgccggtcaa ctaccgcaac
     3301 tacgtcaccg agttcggcaa cagcagcagt tcgattccgc tgacgatcgg ctggtcgcgc
     3361 gacagccgcg acagcgc
//
LOCUS       DATMJB010000478         3212 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_444219, whole genome shotgun sequence.
ACCESSION   DATMJB010000478 DATMJB010000000
VERSION     DATMJB010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444219"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..676
                     /locus_tag="VNU48_10190"
     CDS             206..676
                     /locus_tag="VNU48_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HWI11688.1"
                     /translation="MKPWLKRTLVGVFGASILIGGLSACGHGGHHGNWAASEADSAKM
                     RERMLERVGTQLKLDDAQKQRLATLADKLREQRVALMGATDPRTDFQALMAGPTFDRS
                     GAQTLVEAKTAALRGKSPEVIAAAADFFDGLRPEQQQQVREFIAKRRGGWRDKS"
     gene            714..1403
                     /locus_tag="VNU48_10195"
     CDS             714..1403
                     /locus_tag="VNU48_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI11689.1"
                     /translation="MRRLLLIDDDERLAAPLATFFARYEFALDAATTPSAGLARLREH
                     AYDAAILDVMLPEMDGFELCRTIRRDADAALRALPIIMLTARGDVMDRVVGLEIGADD
                     YLPKPFEPRELLARLQTVLRRARVPADSTRRLRFDGLQIDLERRQVDGGGRAIELTGT
                     EFDLLVLLASEPQKVFNRDEILNRLRGIDAELHTRAVDILISRLRRKLEPLDVIKTLR
                     NAGYTFAGARA"
     gene            1400..2728
                     /locus_tag="VNU48_10200"
     CDS             1400..2728
                     /locus_tag="VNU48_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWI11690.1"
                     /translation="MNRRTLQGSWRRARHSLRARLTGLFLLLALAMTLTLLAGMQAML
                     RDGWQELARPLTGDYIDRLAAEIGTPPDVAKAQALTRRLPLRIRIDGPVVHWSSHPND
                     GHDARGLQRSVDTDRPPSLWRPTRVLADGHRIRFGLASLPRDDDAPEDRARLIGWATL
                     LLLLALVALAYARVRRLLRPLADIRAGAIRYGAGDFSRPIAPRAHDELGELAQQINGM
                     ADGLHHLLDAKRQLLLAISHELRSPLTRARLNAELVDESPSRDALLHDLAEMRDLITD
                     LLESERLAAGHAALQVEAVDLNALLRELVATQFAARGLVLALDAQLPPLALDAPRMRL
                     LLRNLIDNALRHSTDAATPPSVTTRLDGDVVRLAVRDHGPGVDAAQLPLLAQAFYRPD
                     SARQRATGGVGLGLYLCRLVAQAHGGSMTIRNAHPGLEIVVTLPLRSAAA"
     gene            complement(2733..>3212)
                     /locus_tag="VNU48_10205"
     CDS             complement(2733..>3212)
                     /locus_tag="VNU48_10205"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWI11691.1"
                     /translation="DIGDKAPDFTAPAALAGKQYDFSLADSLAKGPVVLYFFPAAFTA
                     GCSAEAHEFAEATDKFAALGATVIGVSTDDIATQLKFSMQACQGKFPVASDEKKAVIK
                     SYDAAMMIMPDFANRVSYVITPDHAIIYSYQSLNPAKHVTNTLAALRDWEKTQKKTQ"
BASE COUNT          473 a         1187 c         1117 g          435 t
ORIGIN      
        1 gggtctcggg cccgcttggg agcggcccgg cgccgggccg gcgcggcgtc gataccgcgc
       61 cgtcggtgct tcacatggcg ccacgcggac gacatcaaac gacacacgcc gataccgcgg
      121 cgagaggacg ccaggcctgc gcgtcggcac gatgaggccg tgttcgaaac acgggcatct
      181 cccctccact ctcgcaagga aaaccatgaa accctggctc aaacgaaccc tcgtcggcgt
      241 gttcggcgcc tcgatcctga tcggcggcct gagcgcctgc ggccacggcg gccaccacgg
      301 caactgggcc gcgagcgagg ccgactcggc gaagatgcgc gaacgcatgc tcgagcgcgt
      361 cggcacgcaa ctcaagctcg acgacgcgca gaaacagcgc ctggccacgc tcgccgacaa
      421 gctgcgcgag cagcgcgtcg cgctgatggg cgcgaccgac ccgcgcaccg atttccaggc
      481 gctgatggcc ggcccgacct tcgatcgcag cggcgcacag accctggtcg aagccaagac
      541 cgcggcgctg cgcggcaaga gccccgaggt gatcgccgcg gccgccgact tcttcgacgg
      601 cctgcggccc gaacaacagc agcaggtgcg cgaattcatc gcgaagcgcc gcggcggctg
      661 gcgcgacaag tcctgagcgc cgcggcgcag cagcgcggcg gacaatcgcg agcatgcgcc
      721 gcctgctgct gatcgacgac gacgagcgcc tcgcggcgcc gctggccacc ttcttcgcgc
      781 gctacgagtt cgcgctcgac gccgcgacga cgccgagcgc gggcctcgcg cggctgcgcg
      841 agcacgcgta cgacgccgcg atcctcgacg tgatgctgcc cgagatggac ggcttcgagc
      901 tgtgccgcac gatccgccgc gacgccgacg cggcgctgcg cgcgctgccg atcatcatgc
      961 tgaccgcgcg cggcgacgtg atggaccgcg tcgtcggcct cgagatcggc gccgacgact
     1021 acctgcccaa gcccttcgag ccgcgcgagt tgctggcccg gctgcagacc gtgctgcggc
     1081 gcgcacgcgt gccggccgac agcaccagac gcctgcgttt cgacgggctg cagatcgacc
     1141 tcgagcgtcg gcaggtcgac ggcggcggcc gcgcgatcga gctgaccggc accgagttcg
     1201 acctgctggt gctactcgcc agcgagccgc agaaggtctt caaccgcgac gagatcctga
     1261 accggctgcg cggcatcgac gccgagctgc acacgcgcgc ggtcgacatc ctgatcagcc
     1321 ggctgcgcag gaagctcgaa ccgctggacg tgatcaagac gctgcgcaac gccggctaca
     1381 ccttcgccgg ggcgcgcgcg tgaaccggcg cacgctgcag ggcagctggc gccgggcccg
     1441 gcactcgctg cgcgcgcgcc tgaccgggct gttcctgctg ctcgcgctgg cgatgacgct
     1501 gaccctgctg gccggcatgc aggcgatgct gcgcgacggc tggcaggagc tcgcgcgccc
     1561 gctgaccggc gactacatcg accgtctcgc cgccgagatc ggcacaccgc ccgatgtcgc
     1621 gaaggcgcag gcgctgacgc ggcggctgcc gctgcgcatc cgcatcgacg ggccggtcgt
     1681 gcactggtcc tcgcatccga acgatgggca cgacgcacgg gggctgcagc gcagcgtcga
     1741 caccgaccga ccgcccagcc tgtggcgccc gacgcgcgtg ctcgccgacg gccaccgcat
     1801 ccgcttcggc ctggcgagcc tgccgcgcga cgacgacgcg cccgaggatc gcgcgcgcct
     1861 gatcggctgg gccacgctgc tgctgctgct ggcgctggtc gcgctcgcgt atgcgcgcgt
     1921 gcgacgcctg ttgcggccgc tcgccgacat ccgcgccggc gcgatccgct acggcgccgg
     1981 cgacttctcg cggccgatcg cgccacgcgc gcacgacgag ctcggcgagc tcgcgcagca
     2041 gatcaacggc atggccgacg ggctgcacca cctgctcgac gcgaagcgcc agctgctgct
     2101 cgcgatcagc cacgaactgc gctcgccgct gacgcgcgcg cggctgaacg ccgagctggt
     2161 cgacgagagc ccgtcgcgcg acgcgctgct gcacgatctg gccgagatgc gcgacctgat
     2221 caccgacctg ctggagagcg agcgcctggc cgccggccat gccgcgctgc aggtcgaagc
     2281 ggtcgacctg aacgcgctgc tgcgcgagct cgtcgcgacg cagttcgccg cacgcggcct
     2341 cgtgctcgcg ctcgatgcac agctgccgcc gctcgcactc gatgcgccgc gcatgcgcct
     2401 gctgctgcgc aacctgatcg acaacgcgct gcgccacagc accgatgccg cgacgccgcc
     2461 gagcgtcacg acgcgcctcg atggcgacgt ggtgcggctg gcggtgcgcg accacggccc
     2521 cggtgtcgac gccgcgcagc tgccgctgct ggcccaggcg ttctatcgcc ccgacagcgc
     2581 gcgccagcgc gccaccggcg gcgtgggtct ggggctgtac ctgtgccgcc tcgtcgcgca
     2641 ggcacatggc ggctcgatga cgatccgcaa cgcccacccg gggctcgaga tcgtcgtgac
     2701 gctgccgctg cgcagcgcag cggcctgaca ggctactgcg tcttcttctg cgtcttttcc
     2761 cagtcgcgca gcgcggccaa cgtgttcgtc acgtgcttgg ccgggttcag gctctggtag
     2821 ctgtagatga tcgcgtggtc gggggtgatc acgtacgaca cgcggttcgc gaagtccggc
     2881 atgatcatca tcgccgcatc gtaggacttg atcaccgcct tcttctcgtc cgaggcgacc
     2941 gggaacttgc cctggcaggc ctgcatcgag aacttcagct gcgtcgcgat gtcgtcggtg
     3001 gacacaccga tcacggtggc gccgagcgcc gcgaacttgt cggtggcctc ggcgaactcg
     3061 tgggcctcgg ccgagcagcc ggcggtgaat gccgccggga agaaatacaa caccaccggc
     3121 cctttcgcga gcgaatcggc gagcgagaag tcgtactgct tgccggccag cgcggccggg
     3181 gcggtgaagt cgggcgcctt gtcgccgatg tc
//
LOCUS       DATMJB010000479         4470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_294163, whole genome shotgun sequence.
ACCESSION   DATMJB010000479 DATMJB010000000
VERSION     DATMJB010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294163"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VNU48_10210"
     CDS             complement(<1..570)
                     /locus_tag="VNU48_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HWI11692.1"
                     /translation="MRFSVYQVSRKGGREKNEDRMGYCYTRDAGLFALADGMGGHPEG
                     EVASQLALQTLAAMFQRDCKPTLPDPLRFLQEATIAGHHQLLRYATQKALIDTPRTTV
                     VACLLQGNAAYWAHCGDSRLYLVRGDKLIARTRDHSYSELQQTMSQVVPMPDRFNRNV
                     LFTCLGSPGKPVIDTVGPLMLQAGDRLLLC"
     gene            complement(576..1544)
                     /locus_tag="VNU48_10215"
     CDS             complement(576..1544)
                     /locus_tag="VNU48_10215"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWI11693.1"
                     /translation="MSKPKPAPLPSGTVVGGYQVIKKLAAGGFGVVYLCEDTDRHLVA
                     LKEYLPSSLAERSPGELTPRVKPEKQPLYRLGLKSFFEEGRSLAQISHPSVVSVLNFF
                     RENETVYMVMNYLQGDTLQDFIVTARDLKRDKVFRESTIRSLFDEILRGLRIVHQHKM
                     LHLDIKPANIFITNENKAVLLDFGAAREVLSKEGNFIRPMYTPGFAAPEMYRRDGTLG
                     PWTDIYAIGACIYACMQGYPPNDAPQRIEKDRLALSLSRLRNVYSDNLIEVTEWCMSL
                     DPLSRPQSVFALQKELARETERRYTKLSFSERLKLQLENLTTGAKA"
     gene            1571..2515
                     /locus_tag="VNU48_10220"
     CDS             1571..2515
                     /locus_tag="VNU48_10220"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HWI11694.1"
                     /translation="MAVYSMTGFASASSGAHSAHETAGAADNLAARSGPPASPGVSIE
                     LRSVNGRFLDLGFRLPDEFRALEPTLRELLSAAFKRGKIELRLSTRSESETLWPQPQP
                     DQLNRLVRLESTVQGWLPKAQGLSVHEILQWCKGSAPSEKLDEAALDAAKRCIAGLRE
                     AREREGARLVKVLMERVERLRELATQAEPLVPVVVKRQQQRFLERWTEALEATGAANT
                     ISREALQERALNEAAAYSIRIDVAEELARLRSHLDEIARLLKAGGEVGKRLDFLIQEL
                     LREANTLGSKAAALELTNISVEMKVAIEQLREQVQNIE"
     gene            2675..2854
                     /locus_tag="VNU48_10225"
     CDS             2675..2854
                     /locus_tag="VNU48_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11695.1"
                     /translation="MKSARDSAARHRRNSEKSGGADGTMPHLPQVRAASISEIDARIE
                     PHSESARAATVSLQF"
     gene            2858..3964
                     /locus_tag="VNU48_10230"
     CDS             2858..3964
                     /locus_tag="VNU48_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system-associated ATPase"
                     /protein_id="HWI11696.1"
                     /translation="MYEAFYGLSSKPFQLNPDPHFYFGSKQHRRAKAYLDYGVSRNDG
                     FIVITGEIGAGKTTILRSLLDSLADSDVVAGHLVTTQLDAEDTLRMVGAAFGFHVQGV
                     PKAELLSSLEAFLITQTSQGKRCLLVVDEAQNLTPRAVEELRMLSNFQLGNQALLQSF
                     LVGQPEFREILQRPEMEQFRQRVAATCHIGPLDLAETEGYIAHRLKCAGAQTDGPEFE
                     AAAIEAIFKASHGIPRRINSVCDRVLLLGYMENRKRLTLADVTEVVREFAEESEVPAR
                     QRATPGHAAEGPDAGATSLASLDIDLALLNLDKDAAADISRRLDGLGAQQGSERLERL
                     EQSVQRLERLNLQTLAALQELVRAIVKPRNGGGS"
     gene            complement(4039..4263)
                     /locus_tag="VNU48_10235"
     CDS             complement(4039..4263)
                     /locus_tag="VNU48_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWI11697.1"
                     /translation="MSWNGSSGATGYRVYYGAASHSYSQALGSGNYVSTTVFTVSGLT
                     PGHTYYFAVTAVGSAGTESAYSNEASKLIP"
BASE COUNT          739 a         1419 c         1577 g          735 t
ORIGIN      
        1 gcacagcagc agccgatcgc cggcctgcag catcagtggg ccgacggtgt cgatcaccgg
       61 cttgcccggg ctgcccaggc aggtgaacag cacgttgcgg ttgaagcggt cgggcatcgg
      121 cacgacctgg ctcatcgtct gctgcagctc ggagtacgag tggtcgcggg tgcgtgcgat
      181 cagcttgtcg ccgcgcacca ggtacaggcg cgaatcgccg cagtgcgccc aataggcagc
      241 gttgccttgc agcaggcagg cgacgacggt ggtgcgcggc gtgtcgatca gcgccttttg
      301 cgtcgcgtag cgcagcagct ggtggtggcc ggcgatcgtc gcttcctgca ggaagcgcag
      361 cgggtcgggc agcgtgggct tgcagtcgcg ctggaacatc gcggccaggg tctgcagcgc
      421 cagctgcgag gcgacctcgc cttcggggtg gccgcccatg ccgtcggcca acgcgaacag
      481 gcccgcgtcg cgcgtgtagc aatagcccat gcggtcttcg ttcttctcgc ggccgccctt
      541 gcggctgacc tggtagacgg agaagcgcat cgtgctcagg ctttcgcgcc ggtggtgagg
      601 ttctcgagct gcagcttcag ccgctcgctg aagctgagct tggtgtagcg gcgctcggtc
      661 tcgcgcgcga gttccttctg cagcgcgaac acgctctgcg ggcgcgacag cgggtcgagc
      721 gacatgcacc actcggtcac ttcgatcagg ttgtcggagt agacattgcg cagccttgag
      781 aggctcagcg cgagacggtc cttctcgatg cgctgcggcg cgtcgttcgg cgggtagccc
      841 tgcatgcagg cgtagatgca ggcgccgatc gcgtagatgt cggtccaggg cccgagcgtg
      901 ccgtcgcggc ggtacatctc gggcgccgcg aaccccggcg tgtacatcgg gcggatgaag
      961 ttgccttcct tgctcagcac ttcgcgcgcc gcgccgaagt ccagcagcac cgccttgttc
     1021 tcgttggtga tgaagatgtt cgccggcttg atgtccaggt gcagcatctt gtgctggtgc
     1081 acgatgcgca agccgcgcag gatctcgtcg aacagcgagc ggatcgtcga ttcgcggaac
     1141 accttgtcgc gcttgaggtc gcgcgcggtg acgatgaaat cctgcagcgt gtcgccctgc
     1201 aggtagttca tcaccatgta gacggtttcg ttctcgcgga agaagttcag caccgagacc
     1261 acgctcgggt ggctgatctg cgcgagcgag cgcccttctt cgaagaagct cttcaggccc
     1321 aggcgataga gcggctgctt ctcgggcttg acgcgcggtg tgagttcgcc gggcgagcgc
     1381 tcggccagcg aggacggcag gtattccttc agcgcgacca ggtgccggtc ggtgtcttcg
     1441 cacaggtaga ccacgccgaa gccgccggcg gcgagcttct tgatgacctg atagccaccc
     1501 accacggtgc cggacggcaa tggggcgggt ttgggctttg acataatccg attttctgaa
     1561 gggtcagcgc atggcagtct acagcatgac cggcttcgcc agcgcctcct ccggggccca
     1621 ttccgcccat gaaacggcgg gcgctgcgga caatttggcg gcgcgctccg ggcctcccgc
     1681 gagccccggt gtcagcatcg aactgcgttc ggtcaacggc cggtttctcg acctggggtt
     1741 tcgtctgccg gacgagttcc gcgcgctcga accgacactg cgcgagttgc tctcggccgc
     1801 cttcaagcgc ggcaagatcg agctgcgcct gagcacgcgc agcgagagcg agacgctctg
     1861 gccgcagccg cagcccgacc agctcaatcg cctggtgcgg ctggagagca cggtgcaggg
     1921 ctggctgccg aaggcgcagg gcctgtcggt gcacgagatc ctgcagtggt gcaagggcag
     1981 cgcgccgtcc gagaagctcg acgaggccgc gctcgacgcg gcgaagcgct gcatcgccgg
     2041 gctgcgcgag gcgcgcgagc gcgaaggcgc gcgcctggtc aaggtgctga tggagcgcgt
     2101 cgagcggctg cgcgaactcg ccacccaagc cgagccgctg gtgccggtcg tcgtcaaacg
     2161 ccagcagcag cgctttctgg agcgctggac cgaggcgctc gaggccaccg gcgcggccaa
     2221 cacgatctcg cgcgaggcgc tgcaggaacg cgcgctgaac gaggctgccg cctactcgat
     2281 ccgcatcgac gtggccgagg aactcgcgcg gctgcgctcg cacctcgacg agatcgcccg
     2341 gctgctgaag gccggcggcg aggtcggcaa gcggctggac ttcctgatcc aggagctgct
     2401 gcgcgaggcc aacacgctgg gctcgaaggc cgcggcgctg gagctgacca acatctcggt
     2461 cgagatgaag gtcgcgatcg agcagttgcg ggagcaggtg cagaacatag agtgacgttt
     2521 cccgcgctgt cgtgccactc cggaagcgcc gattgagccc ttgaacgtcg atggagttgc
     2581 acgcgatgcg gccagttacc tttcgcccac gcggccgtag cgcaccaagc gcgtgcccca
     2641 tactcagggg atggttccgg atagcgatgt tgaggtgaaa tcggcccgcg actcggccgc
     2701 ccggcatcgg cgcaacagtg agaagtcagg cggtgccgac ggcactatgc cgcacctgcc
     2761 gcaggttcgg gctgcatcga tctcggagat cgatgctcga atagaacccc attccgaatc
     2821 cgcgcgcgcg gccaccgtca gtttgcagtt ctgagcaatg tacgaagcct tctacggatt
     2881 gagcagcaag ccgttccagc tcaaccccga cccgcacttc tatttcggca gcaaacagca
     2941 tcgccgtgcc aaggcgtacc tcgactatgg ggtctcgcgc aacgatggct tcatcgtgat
     3001 caccggcgag atcggcgccg gcaagacaac gatcctgcgc agcctgctcg acagcctggc
     3061 cgacagcgat gtcgtcgccg ggcacctggt gacgacgcaa ctcgacgccg aagacacgct
     3121 gcgcatggtc ggcgcggcgt tcggctttca cgtccagggt gtcccgaagg ccgaattgct
     3181 ctcgtcgctg gaggcatttc tcatcacgca gaccagccag ggcaagcgct gcctgctggt
     3241 ggtcgacgaa gcccagaacc tgacgccacg cgcggtcgag gaactgcgca tgctgtcgaa
     3301 tttccagctc ggcaaccagg cattgctgca aagctttctg gtcggccagc cggaattccg
     3361 cgagatcctg cagcgtccgg agatggaaca gttccgccag cgcgtggccg cgacctgcca
     3421 catcggcccg ctcgatctgg ccgagaccga aggctacatc gcccaccggc tcaagtgtgc
     3481 gggcgcccag acggacggcc cggagttcga ggccgcggcg atcgaggcca ttttcaaggc
     3541 aagtcatggg attccgcgcc gcatcaattc ggtgtgcgac cgggttctcc ttctgggcta
     3601 catggagaac cgcaaacgac tcacgcttgc cgacgtgacg gaagtcgtgc gtgagttcgc
     3661 cgaagagtcc gaggtgccgg cgcgtcaacg cgccacgccc ggccatgcgg cggagggccc
     3721 ggacgcgggt gccacgtcgt tggcatcgct cgatatcgac ctggcgctgc tcaatctcga
     3781 caaggacgcc gccgcggaca tatcgcgccg gctcgacggt ctgggcgcgc agcagggcag
     3841 cgaacgcctg gagcgcctgg agcaaagcgt tcagcgcctc gaacgcctga atttgcaaac
     3901 cctggccgcg ttgcaggagt tggtacgcgc gatcgtcaag ccgcgcaacg gaggcgggtc
     3961 atgaagacgc gctctcggcg ccggcagccg gagtagtccc gtgcgtgtcg gcgcccggcc
     4021 gcaccgtcac aggctcgctc atgggatcag tttggacgct tcgttcgaat aggcgctctc
     4081 tgtaccggcg gagccaacag ccgtgacggc aaagtagtag gtgtgcccag gcgtcaggcc
     4141 agataccgtg aagaccgtcg tgctcacgta gttgccgcta cccaatgctt ggctgtagct
     4201 gtggctcgcg gcgccgtagt acacgcgata acccgtggcc cctgacgagc cattccagga
     4261 cagcgtggct gtgccagccg gagccggagc cggagccgga gccggagccg gagcgggagc
     4321 gggagcggga gcgggtgcgg gagcgggtgc gggagccgga gccggagccg gagccggagc
     4381 cggagccgga gccggagcgg gtgcgggtgc gggtgcgggt gcgggtgcgg gagcgggtgc
     4441 gggtgcgggt gcgggtgcgg gagcgggagc
//
LOCUS       DATMJB010000480         2523 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_195730, whole genome shotgun sequence.
ACCESSION   DATMJB010000480 DATMJB010000000
VERSION     DATMJB010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195730"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VNU48_10240"
     CDS             complement(<1..432)
                     /locus_tag="VNU48_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11698.1"
                     /translation="MRLTLESTVAEPTRWAVIAARIAAWADEHGVALRDAIVLLPFAQ
                     LLPEARAAFTQRGGWMPRIETTRTLAASLGPAAPLRVGELSFDPTLDALSASALLCSR
                     PWGAAWARRDARGFEQAVHDLVLGAQVLLQAAAALPPRARAA"
     gene            556..885
                     /gene="trxA"
                     /locus_tag="VNU48_10245"
     CDS             556..885
                     /gene="trxA"
                     /locus_tag="VNU48_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HWI11699.1"
                     /translation="MSSELIKHTSDASFDTDVLKASKPVLVDYWAEWCGPCKMIAPIL
                     DEVSKDYDGRLQIAKMNVDENRDVPAKFGIRGIPTLMLFKDGQLAATKVGALSKAQLT
                     AFLDGHL"
     gene            1132..2394
                     /gene="rho"
                     /locus_tag="VNU48_10250"
     CDS             1132..2394
                     /gene="rho"
                     /locus_tag="VNU48_10250"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWI11700.1"
                     /translation="MHLSELKLIHVSELITMGEALEIENVARLRKQELMFAIMKKRAK
                     AGEQVFGDGVLEVLPDGFGFLRSPDASYMASTDDIYLSPSQIRRFNLHTGDAIEGEVR
                     VPKDGERYFALVKVDRVNGVAPEESKHKIMFENLTPLFPKEQFKLEREIKSDENIMSR
                     IIDLIAPIGKGQRALLVAQPKTGKTVMMQQMAHALVANHPDVHLIVLLVDERPEEVTE
                     MVRTVRGEVISSTFDEPAARHVQVAEMVIERAKRLVEMKKDVVILLDSITRLARAYNN
                     VLPSSGKVLTGGMDSNALQRPKRFFGAARNVEEGGSLTIIGTALVDTGSRMDEVIYEE
                     FKGTGNCEIHLDRRMAEKRVYPSILLNKSGTRREELLLQPEILQKTWILRKLLYPMDE
                     IEAMEFILDKMKSTKNNHDFFDMMRRGG"
BASE COUNT          487 a          823 c          827 g          386 t
ORIGIN      
        1 ggcggcgcgc gcgcgcggcg gcagcgcggc agcggcctgc agcagcactt gcgcgccgag
       61 cacgaggtcg tgcacggcct gctcgaagcc gcgcgcatcg cggcgtgccc aggccgcacc
      121 ccagggccgc gagcacagca aggccgaggc gctgagcgcg tcgagggtcg gatcgaagct
      181 gagctcgccg acccgcagcg gcgccgcggg gccaaggctc gcggcgaggg ttcgcgtggt
      241 ttcgatgcgc ggcatccagc cgccgcgttg cgtgaaggcg gcacgcgctt cgggcagcag
      301 ttgcgcgaac ggcagcagca cgatcgcgtc gcgcagcgcg acgccgtgct cgtcggccca
      361 ggccgcgatg cgcgctgcaa tgacggccca gcgcgtcggc tcggcaacgg tgctttcaag
      421 ggtcagccgc actatcgttg ccataggaag gaagacggcc gaagacgttg gcctgcaccg
      481 cggatggaag ggctttgtgt cagaatcatt gtgccttgcc acccacttca tgcggtgcag
      541 ccttaaggac aaaccatgag cagcgaactg atcaagcaca cttccgacgc ctcgttcgac
      601 accgacgtgc tgaaggcttc caagccggtg ttggtcgact actgggcgga atggtgcggc
      661 ccgtgcaaga tgattgcgcc gatcctagac gaggtctcga aggactacga cggccgcctg
      721 cagatcgcca agatgaacgt cgacgagaac cgcgacgtcc cggcgaagtt cggcatccgc
      781 ggcatcccga cgctgatgct gttcaaggac ggccagctcg ccgcgaccaa ggtcggcgca
      841 ctgtcgaagg cccagttgac cgcgttcctc gacggtcatc tataatcctg cgaaacgcgg
      901 cgggagccgg cgccttaccc aagccgccgg tttcgattgc tgagagtccg ccgccagaat
      961 ttgccaccac gccccgaggc ccgctgagac ggtttcagcg gccccgacgc ccgaaccggc
     1021 ggcgcgtgga cccggcaccc cactcccgct gttgttcgcc gttcgcttcg cgagcccgct
     1081 tgcccttgcc ggctccggcc tgtcgccatc cacacccagg gtcattcgcc catgcatctg
     1141 tcagaactca agctgatcca cgtctcggag ctcatcacga tgggcgaggc gttggagatc
     1201 gagaacgtcg cccgcctgcg caagcaggag ctgatgttcg cgatcatgaa gaagcgcgcc
     1261 aaggccggcg agcaggtgtt cggcgacggc gtgctcgagg tgctgcccga cggcttcggc
     1321 ttcctgcgct cgcccgacgc gagctacatg gccagcaccg acgacatcta cctcagcccg
     1381 agccagatcc ggcgcttcaa cctgcacacc ggcgacgcga tcgaaggcga ggtgcgggtg
     1441 cccaaggacg gcgaacgcta cttcgcgctc gtcaaggtcg accgcgtgaa cggcgtcgcg
     1501 cccgaggaga gcaagcacaa gatcatgttc gagaacttga cgcccctgtt cccgaaggag
     1561 cagttcaagc tcgagcgcga gatcaagtcg gacgagaaca tcatgagtcg catcatcgac
     1621 ctgatcgcgc cgatcggcaa aggccagcgc gcattgctgg tcgcgcagcc gaagaccggc
     1681 aagaccgtga tgatgcagca gatggcgcac gcgctggtcg cgaaccaccc cgacgtgcac
     1741 ctgatcgtgc tgctggtcga cgagcggccc gaagaagtga cggaaatggt ccgcacggtg
     1801 cgcggtgaag tcatcagctc gaccttcgac gagcccgcgg cgcgccacgt ccaggttgcc
     1861 gaaatggtga tcgagcgcgc caagcgcctg gtcgagatga agaaggatgt ggtcatcctg
     1921 ctcgactcga tcacccggtt ggcgcgggcc tacaacaacg tgctgccgtc gagcggcaag
     1981 gtgctgaccg gcggcatgga cagcaacgcg ctgcaacgcc cgaagcgctt cttcggcgcg
     2041 gcgcgcaacg tcgaggaagg cggatccctc accatcatcg gcaccgcgct cgtcgacacc
     2101 ggctcgcgca tggacgaggt gatctacgag gagttcaagg gcaccggcaa ctgcgaaatc
     2161 cacctcgatc gccgcatggc cgagaagcgc gtctacccgt cgatcctgct caacaagagc
     2221 ggcacgcgcc gcgaggaact gctgctgcag cccgagatcc tgcaaaagac ctggatcctg
     2281 cgcaagctgc tgtacccgat ggacgagatc gaggcgatgg agttcatcct cgacaagatg
     2341 aagtcgacca agaacaacca cgacttcttc gacatgatgc gccggggcgg ctgagtatcg
     2401 ggcctgggtt ataattttag gtttcccgca tcgcggaagt ttggatttcc gccaacggaa
     2461 agtgcgtcga atggttcggc gtggctcccg caacgactgg caagagaagg cgacgaggtt
     2521 ccc
//
LOCUS       DATMJB010000481         3058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_493211, whole genome shotgun sequence.
ACCESSION   DATMJB010000481 DATMJB010000000
VERSION     DATMJB010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493211"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1618
                     /locus_tag="VNU48_10255"
     CDS             17..1618
                     /locus_tag="VNU48_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HWI11701.1"
                     /translation="MPVFGRNVVATSHPLAAQAGLQMLRAGGNAIDAAIATAAAMTIL
                     EPVSNGLGSDAFCLLWDGKTLHGLNASGPAPRAWTPEYFSRRHGAGAAAPPQRGWDSV
                     TVPGAVAAWVAMSERFGRLPFADLLAPACEIAERGYAVPVVVQQKWAAAVPLLADQPG
                     FAQAFMPRGRAPQVGELFRFPDAARTLRLIAQSKGAAYYGGEIAQAAAQHAQSHGGAM
                     TTDDFAAYRPEWVTPIGMACADRSGAGHTLHEIPPNGQGIAALIALGILQHFDLAALP
                     PDSTRAQHLQIEAMKLAFADTYRFVAEPASMEATSAQLLDPTYLKARAKLIDPKRAQD
                     FGHGNPAKGGTIYLSAADERGMMVSFIQSNYMGFGSGVVVPGYGVSMQNRGHGFSLVA
                     GSPNLVAPGKRPFHTIIPAFLTRGAGDAREAVMSFGVMGANMQPQGHLQTLLRMLVHG
                     QNPQAACDAPRWRFNTGLEINVEAAMDPATAAGLQALGHRVEVINDSYQDFGAGQFIW
                     RLGDPAVEGYVAASDPRRDGQAVAY"
     gene            1676..2602
                     /locus_tag="VNU48_10260"
     CDS             1676..2602
                     /locus_tag="VNU48_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWI11702.1"
                     /translation="MSSTVSSAAAGAAAVRERAVWPGLLLASLGAIAFSGKAIIVKLA
                     YRYGVDAVTLIMYRMLFALPLFMLLAWWAGRGKPALTRRDWIVVVGLGFTGYYLASFL
                     DFAGLAYVTAGFERLILYLNPTLVLAMGAVLFKRQVTGRQLIALGVSYCGVLLVFGHE
                     LSALGSHVALGAALVFASAVSYAIYLVYSGEEVRRLGALRLTGLATTVACLFCIAQFA
                     LLRPMSALAVAPEVIWLSMLNATLCTFAPVLMVMMAIERIGATIAAQTGMIGPLSTIL
                     MGVVILGEPFTVWIAAGTVLVLAGIWLLAKWR"
     gene            2607..>3058
                     /locus_tag="VNU48_10265"
     CDS             2607..>3058
                     /locus_tag="VNU48_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI11703.1"
                     /translation="MDLGIAGKWALVCAASKGLGKGCASALVREGVNVVITARGADAL
                     EATATELRALARERGGGEVRAVAGDITTAEGRLAALAACPQVDILINNAGGPPALLTR
                     MSTCGHAASAAARPAGVVMSPTTCTTWTPGDATAISAAVACSVSAPRAV"
BASE COUNT          406 a         1068 c         1124 g          460 t
ORIGIN      
        1 ctaccccagc cagcgcctgc cggtcttcgg ccgcaacgtc gtcgccacct cgcacccgct
       61 ggccgcacag gccgggctgc agatgctgcg tgcgggcggc aacgcgatcg acgcggcgat
      121 cgcgaccgct gccgcgatga ccatcctcga gccggtcagc aacggcctcg gctccgacgc
      181 gttctgcctg ctgtgggacg gcaagacact gcacggcctg aacgcctcgg gccccgcgcc
      241 gcgcgcgtgg acgcccgagt acttcagccg caggcacggc gccggcgcag cggcgccgcc
      301 ccagcgcggc tgggacagcg tgaccgtgcc gggggcggtc gcggcctggg tcgcgatgag
      361 cgaacgcttc ggccgcttgc cgttcgcgga cctgctggcg ccggcgtgtg agatcgccga
      421 acgcggctat gcggtgccgg tggtcgtgca gcagaagtgg gccgccgcgg tgccgctgct
      481 cgccgaccag ccgggcttcg cgcaggcgtt catgccgcgc ggccgggcac cgcaggtcgg
      541 cgagttgttc cgcttccccg atgccgcccg cacgctgcgc ctgatcgcgc agtcgaaggg
      601 cgcggcgtac tacggcggtg agatcgcgca ggccgcggcg cagcatgccc aatcgcacgg
      661 cggcgcgatg accaccgacg actttgcggc ctaccggccc gagtgggtca cgcccatcgg
      721 catggcctgc gccgaccgca gcggtgcggg ccacacgctg cacgagatcc cgccgaacgg
      781 gcagggcatc gcggcgctga tcgcgctggg catcctgcag cacttcgacc tcgcggcctt
      841 gccgcccgac agcacgcgag cgcagcacct gcagatcgag gcgatgaagc tggccttcgc
      901 cgacacctat cgcttcgtcg ccgaacccgc gagcatggag gcgacgagcg cgcagctgct
      961 ggacccgacg tatctgaagg cgcgcgcgaa gctgatcgac ccgaagcggg cgcaggactt
     1021 cggccacggc aacccggcca agggcggcac gatctacctg agcgcggccg acgagcgcgg
     1081 catgatggtc agcttcatcc agagcaacta catgggcttc ggctccggcg tggtggtgcc
     1141 gggttacggc gtgtcgatgc agaaccgcgg gcatggcttc agcctggtcg ccggcagccc
     1201 gaacctcgtc gcgccgggca agcgcccgtt ccacaccatc atcccggcgt tcctgacgcg
     1261 cggcgccggc gacgcgcgcg aggccgtgat gagcttcggc gtgatgggcg cgaacatgca
     1321 gccgcagggc cacctgcaga cgctgctgcg catgctcgtg cacggccaga atccgcaggc
     1381 cgcctgcgac gcgccgcgct ggcgcttcaa cactgggctc gagatcaacg tcgaggcggc
     1441 gatggacccg gccaccgccg ccgggctgca ggcactgggc caccgcgtcg aggtcatcaa
     1501 cgacagctac caggacttcg gcgccggtca gttcatctgg cgcctcggcg acccggcagt
     1561 cgagggctac gtggcggcga gcgatccacg ccgcgacggg caggcggtcg catactgagt
     1621 tcgcgaccgc gccggtgccg gcgcgaagcg caggcgctcc gtagaatcgg ccgcaatgtc
     1681 ttccaccgtc tcctcggcag ccgccggcgc tgccgccgtg cgcgagcgcg cggtctggcc
     1741 cggcctgctg ctggcgtcgc tcggtgcgat cgcgttctcc ggtaaggcga tcatcgtgaa
     1801 gctcgcgtac cgctacggcg tcgacgcggt caccttgatc atgtaccgca tgctgttcgc
     1861 gctgccgctg ttcatgctgc tggcctggtg ggccgggcgc ggcaagccgg cgctgacgcg
     1921 gcgcgactgg atcgtcgtcg tcggtctggg cttcaccggc tactacctgg cgagctttct
     1981 cgacttcgcg gggctggcct atgtcacggc cgggttcgaa cgcctgatcc tgtacctgaa
     2041 cccgacgctg gtgctggcga tgggcgctgt gctgttcaag cgccaggtga cgggccggca
     2101 gctgatcgcg ctcggcgtca gctattgcgg cgtgctgctg gtgttcggcc acgagctcag
     2161 cgcgctcggc tcgcatgtgg cgctcggcgc ggcgctggtg ttcgccagtg cggtgagcta
     2221 tgcgatctac ctcgtctaca gcggcgagga ggtcaggcgc ctcggtgcgc tgcggctcac
     2281 gggcctggcg accaccgtcg cgtgcctgtt ctgcatcgcg cagttcgcgc tgctgcggcc
     2341 gatgagcgcg ctcgcggtcg cgcccgaggt gatctggctg tcgatgctga acgccacgct
     2401 gtgcaccttt gcgccggtgc tgatggtgat gatggcgatc gagcgcatcg gcgcgacgat
     2461 cgcggcgcag accggcatga tcggcccgct gtcgaccatc ctgatgggcg tggtgattct
     2521 cggcgagccg ttcaccgtgt ggatcgcggc cggcacggtg ctggtgctgg ccgggatctg
     2581 gctcctggcg aagtggaggt gaggcgatgg atctcggtat agcagggaag tgggcgctgg
     2641 tgtgcgcggc cagcaagggc ctgggcaagg gctgcgcgag cgcgctggtg cgcgaaggcg
     2701 tcaacgtcgt gatcacggcg cgcggcgccg acgcgctcga agcgaccgcc accgagctgc
     2761 gcgcgctcgc ccgcgaacgc ggcggtggcg aagtgcgcgc ggtggccggc gacatcacca
     2821 ccgccgaggg ccgcctcgcc gcgctggcgg cgtgcccgca ggtcgacatc ctgatcaaca
     2881 acgccggcgg cccgccggcg ttgttgacca ggatgtcgac ctgcgggcac gccgccagcg
     2941 cggcggcgcg gccggcgggt gtcgtgatgt cgcccaccac ctgcacgacc tggacgccgg
     3001 gcgatgcgac ggcgatttcg gcggcggttg cctgcagcgt atcggcgccg cgcgccgt
//
LOCUS       DATMJB010000482         6163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_255823, whole genome shotgun sequence.
ACCESSION   DATMJB010000482 DATMJB010000000
VERSION     DATMJB010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255823"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VNU48_10270"
     CDS             complement(<1..472)
                     /locus_tag="VNU48_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HWI11704.1"
                     /translation="MVNPTIVRARARWVPVDWADLPGWETDRVSELWPALLRGCERPA
                     FGWEALCAQARNAATPAFSDDAARAWLRQRLQPYRIESLEGNADGLLTGYFEPLVEAT
                     RTARGAHQVPLYGPPADLATRKPYWTRQQLDSSSAAQASVRGRALAYVADPLDAL"
     gene            465..719
                     /locus_tag="VNU48_10275"
     CDS             465..719
                     /locus_tag="VNU48_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11705.1"
                     /translation="MTIGTASGLVAAPGTSTGAGARGGGLEQPASNPRIAAGSSALRT
                     FDMWVLFLEAFGAVLLLVLIVWWTMFHGRRNGERDDGGDS"
     gene            complement(720..2789)
                     /locus_tag="VNU48_10280"
     CDS             complement(720..2789)
                     /locus_tag="VNU48_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HWI11706.1"
                     /translation="MPKAKPRVSPWDLTALLNAADPKASLPERHLWLIRLLEWLRHAP
                     VEAARDAATDIDTNATPRPVLRLRHLLGVLERHPEHQGRVVELLQRIWAEVDNAALLA
                     DFGFAPRMDLWGELGRRIRARVLPLTPATTDLGELFALLFPHADDAAWLLAIDDATLE
                     RLRGFIAPQRAGHAWRAPFLDAIVFLASAVRAAGFAPALRLRMSPELLVDRPFRQLAR
                     IAEQVSDHAQALSADPQTAANAPSEGQPTPATEAALLQEAQYLRALLAQCRRCAESVH
                     EHLEAHGVSVDVVFEVDQLRARTHRIDALLTCVVSPEPAREIARLIAELVQTADERRS
                     IRGLMAEHYSLLARKVAERSAETGQHYITRTLAEYRRMLRAAAGGGAVLAATTFIKFA
                     VLALALSPFWGGFWAGVNYAASFVLIQLLHFTVATKQPAMTAPAMAEKLADLSTDEHV
                     EGFVDEVAQLIRSQAAGIFGNLALVAPIVLAVQFGWQAAFGRPLVGHADAQHVLESLT
                     LLGATPLFAAFTGVLLFASSLIAGWVENWFVWHRLDSAIAWNPRIVARLGSARAQRWG
                     AYWRANISGFASNTSLGLMLGIVPVVALFFGVPLEVRHVTLSTGQLAAAVGAEGFALL
                     RHAPFWWCVAAVAVTGVLNLTVSFLMAFRVALRSRGIRLVDRSRIYRAIRARLWRKPL
                     SFLWPAE"
     gene            complement(2789..3175)
                     /gene="apaG"
                     /locus_tag="VNU48_10285"
     CDS             complement(2789..3175)
                     /gene="apaG"
                     /locus_tag="VNU48_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HWI11707.1"
                     /translation="MPNPERKAELTSTVSVRHLPEQSDAASGLHAFAYTVTIRNSGNV
                     TAQLIARHWIITDANGHTDEVRGLAVVGHQPLLKPGETFEYTSGTRLATPHGTMRGTF
                     FCMTEDAQPFDAPVGEFGLTLASALH"
     gene            3259..3948
                     /gene="rpe"
                     /locus_tag="VNU48_10290"
     CDS             3259..3948
                     /gene="rpe"
                     /locus_tag="VNU48_10290"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWI11708.1"
                     /translation="MPTPRIYRIAPSILSADFARLGDELRDVIAAGADWIHFDVMDNH
                     YVPNLSFGPMVCEALRKHAVKPDGTKVPIDVHLMVQPVDALAVAFAQAGADRISFHPA
                     ASAHVDRTIQLIRAEGCSAGLVFNPAASLDELEWTIDEIDLVLVMSVNPGFGGQSFIP
                     GALKKIERIRTLIDASGRDIRLEVDGGIKVDNIRAAADAGADTFVAGSAIFGQPDYGA
                     VIKAMRARLAG"
     gene            4021..5028
                     /locus_tag="VNU48_10295"
     CDS             4021..5028
                     /locus_tag="VNU48_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWI11709.1"
                     /translation="MADLHAHHDHRHGHAEAHDHAHHGHDHAHGHGHHHAPASFDRAF
                     AIGISVNILFVAIEAFYGWKVNSLALLADAGHNLGDVAGLVMAWGGALAGKLRPDARY
                     TYGWKRASILAAFANALLLLVAMGSLAWEAIGRLSAPEPIQGITVMVVAGIGIVVNAG
                     TALLFMRGRDDDMNIRGAYLHMASDALVSAGVVVVGALALWTGWAWLDPVASLLIAFV
                     IVVGTWGLFRQSLRLMFDAVPEHIDLRAVRGMLEALPGVARVHDLHVWATGTTDVVLT
                     AHLVMPAGHPDDPFFEGATHQLRERFKIGHVTLQVVREPFMALCEGAPDAPDLRPAHR
                     H"
     gene            5043..6014
                     /locus_tag="VNU48_10300"
     CDS             5043..6014
                     /locus_tag="VNU48_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HWI11710.1"
                     /translation="MEFKDYYKTLGVARDASPDEIKSAYRKLARKYHPDVSKEPQAEA
                     RFKDIGEASEVLRDPEKRAAYDAAGAQWERQQAAGAAGRPQDFQPPPGWDTGFEFSGG
                     EEDLFGGADHSDFFEALFGRAARAGGGRARRPDVQMPGQDHHAKVMIDLEDAFHGAQR
                     SISLRMPALDDEGRGVLRERTLDVNIPKGIREGQHLRLAGQGGPGLGNAPAGDLFLEI
                     VFKPHPRYRVDGRDVYVDLPLAPWEAALGAAVGAATPDGEVELTIPPGSAAGRKLRLK
                     GRGIPGQPPGDLYVVLAIALPPATDAKARAAYQAFAQAFEFDPRAPK"
BASE COUNT          916 a         2169 c         2252 g          826 t
ORIGIN      
        1 tcagcgcgtc gagcgggtcg gccacgtacg cgagcgcacg gccgcgcacg ctggcctgcg
       61 cggcggacga gctgtcgagc tgctggcgcg tccagtaggg cttgcgcgtg gcgaggtcgg
      121 cgggcggccc gtacagcggt acctggtgcg cgccgcgcgc tgtgcgcgtg gcctcgacca
      181 gcggctcgaa gtagccggtc agcaggccgt cggcgttgcc ttcgagcgac tcgatgcggt
      241 acggctgcag gcgctggcgc agccaggcgc gcgctgcgtc gtcgctgaac gccggcgtcg
      301 ccgcattgcg cgcctgggcg caaagggctt cccagccgaa ggcggggcgc tcgcagccgc
      361 gcagcagcgc cggccacagc tcgctgacgc ggtcggtctc ccagcccggc agatcggccc
      421 agtcgaccgg cacccagcgc gcccgggcac gcacgatcgt cgggttgacc atcggcacgg
      481 ccagcgggtt ggtcgcggcg ccgggcacat cgacgggcgc cggtgcgcgc ggtggcgggc
      541 tggagcagcc cgccagcaac cctagaatcg ccgccggcag cagcgccctg aggacattcg
      601 acatgtgggt actctttctg gaagcattcg gcgccgtgtt gctgctggtg ttgattgtct
      661 ggtggacgat gtttcacggc cgtcgcaacg gcgagcgtga cgacggcggc gacagctgat
      721 cactcggcgg gccagaggaa cgacagcggc ttgcgccaca gccgcgcgcg gatcgcccgg
      781 tagatgcggc tgcggtcgac cagacggatg ccgcgcgaac gcagcgcgac gcgaaacgcc
      841 atcaggaaac tcaccgtcag gttcagcacg ccggtcaccg cgacggcggc cacgcaccac
      901 cagaacggcg cgtggcgcag cagcgcgaag ccctcggcgc ccaccgccgc ggcgagctgg
      961 ccggtcgaga gcgtcacgtg ccgcacctcc agcggcacgc cgaagaagag ggccacgacc
     1021 ggaacgatgc ccagcatcag ccccagcgag gtgttcgacg cgaagcccga gatgttggcg
     1081 cgccagtacg caccccagcg ctgcgcccgg gccgagccca ggcgcgcgac gatgcgcggg
     1141 ttccaggcga tcgcgctgtc cagccggtgc cagacgaacc agttctcgac ccagccggcg
     1201 atcagcgagc tcgcgaacag cagcacgccg gtgaacgcgg cgaacagcgg cgtggcgccg
     1261 agcagcgtca gcgactccag cacgtgctgc gcgtcggcgt gcccgaccag cggccggccg
     1321 aacgcggcct gccagccgaa ctgcaccgcc agcacgatcg gcgcgaccag cgcgaggttg
     1381 ccgaagatgc ccgcggcctg cgagcggatc agctgcgcga cctcgtcgac gaagccctcg
     1441 acgtgttcgt cggtggacag gtcggcgagc ttctcggcca tcgccggcgc cgtcatcgcc
     1501 ggctgcttgg tcgccaccgt gaagtgcagc agctggatca gcacgaagct cgccgcgtag
     1561 ttcacgccgg cccagaaacc gccccagaac ggcgacagcg ccagcgccag caccgcgaac
     1621 ttgatgaagg tggtcgccgc cagcaccgcg ccgccgccgg ccgcggcgcg cagcatccgg
     1681 cggtattcgg ccagcgtgcg cgtgatgtag tgctggccgg tctcggcgct gcgctccgcg
     1741 accttgcgcg cgagcagcga gtagtgctcg gccatcaggc cgcggatgct gcgccgttcg
     1801 tcggccgttt gcacgagctc ggcgatcagc cgcgcgatct cgcgcgccgg ctccgggctg
     1861 acgacgcagg tcagcagcgc gtcgatgcga tgcgtgcgcg cgcgcagctg gtcgacctcg
     1921 aacaccacgt cgaccgagac gccgtgcgcc tcgaggtgct cgtgcacgct ctcggcgcag
     1981 cgccggcact gcgccagcag cgcgcgcagg tactgcgcct cctgcagcag tgcggcctcg
     2041 gtggccggcg tgggctggcc ttcgctcggc gcgttcgccg cggtctgcgg atcggcgctg
     2101 agcgcctggg cgtggtcgct gacctgttcg gcgatgcgcg cgagctggcg gaacggccgg
     2161 tcgaccagca gctcgggact catgcgcagg cgcagcgccg gcgcgaagcc cgcggcccgc
     2221 accgcactgg cgaggaacac gatcgcatcg aggaagggcg cgcgccaggc gtggccggcg
     2281 cgctgcggcg cgatgaagcc gcgcagccgc tccagcgtcg cgtcgtcgat cgcgagcagc
     2341 caggcggcgt cgtcggcatg cgggaacagc agcgcgaaca gctcgccgag gtcggtcgtc
     2401 gccggtgtca gcggcagcac gcgggcgcgg atgcgccggc cgagctcacc ccacaggtcc
     2461 atgcgcggcg cgaagccgaa gtcggccagc agcgcggcgt tgtcgacctc ggcccagatg
     2521 cgctgcagca gctcgacgac gcggccctga tgctcagggt gacgctcgag cacgcccagc
     2581 aggtggcgca ggcgcagcac cgggcgcggc gtggcgttcg tgtcgatgtc ggtggcggcg
     2641 tcgcgagcgg cctcgacggg tgcgtgacgc agccattcga gcagccggat cagccagagg
     2701 tgccgctcgg gcaggctcgc cttcgggtcg gccgcgttca gcagcgcggt caggtcccag
     2761 ggcgacaccc ggggcttggc cttcggcatc aatgcagtgc cgaggccaag gtcaggccga
     2821 actcgccgac cggcgcgtcg aagggctgcg cgtcctcggt catgcagaag aaggtgccgc
     2881 gcatcgtgcc gtgcggtgtg gccaggcgcg tgccgctcgt gtactcgaag gtctcgccgg
     2941 gcttgagcag cggctggtgg ccgaccaccg cgagaccgcg cacctcgtcg gtgtgcccgt
     3001 tcgcgtcggt gatgatccag tggcgcgcga tcagctgcgc ggtgacgttg ccgctgttgc
     3061 ggatcgtcac ggtgtacgcg aaggcgtgca ggccgctcgc cgcgtcggat tgctcgggca
     3121 ggtggcgcac gctgaccgtg ctggtcagtt cggctttgcg ttcggggttc ggcatgcggc
     3181 cgattctggc agcaacaggg ctgacaccta gaatcccggc cattccccaa agcgctcatg
     3241 cggcgcgaac gacccgccat gccgacccca cgcatctacc gcatcgcccc gagcattctg
     3301 tcggcagatt tcgcgcgcct cggcgacgag ctgcgcgacg tgatcgcggc cggcgccgac
     3361 tggatccact tcgatgtgat ggacaaccac tacgtgccga acctgagctt cggcccgatg
     3421 gtgtgcgagg cgctgcgcaa gcatgcggtg aagcccgatg gcacgaaggt gccgatcgac
     3481 gtgcacctga tggtgcagcc ggtcgacgcg ctcgcggtcg cgttcgcgca ggccggcgcc
     3541 gaccgcatca gcttccaccc cgcggccagc gcgcacgtgg accgcacgat ccagctgatc
     3601 cgcgccgagg gctgcagcgc cgggctggtg ttcaacccgg ccgcgtcgct cgacgagctc
     3661 gagtggacca tcgacgagat cgacctggtg ctggtgatga gcgtgaaccc cggcttcggc
     3721 gggcagagct tcattcccgg cgcgctgaag aagatcgagc gcatccgcac gctgatcgac
     3781 gcgagcgggc gcgacatccg gctcgaggtc gacggcggca tcaaggtcga caacatccgc
     3841 gccgccgccg atgccggcgc cgacaccttc gtcgccggca gcgcgatctt cgggcagccc
     3901 gactacggcg cggtgatcaa agcgatgcgc gcgcgcctgg ccggctgagc ggcgcgcggt
     3961 cgacccggcg ccatgggccg gcgcaaaccg ggcgatccaa agctgagtca cactggcggc
     4021 atggcagacc tgcacgcgca ccacgaccac cgccacggcc acgccgaggc gcacgaccat
     4081 gcgcatcacg ggcacgacca cgcgcacggc catggccacc accacgcacc ggccagcttc
     4141 gaccgcgcgt tcgcgatcgg catctcggtc aacatcctgt tcgtcgcgat cgaggccttc
     4201 tacggctgga aggtcaactc gctcgcgctg ctggccgacg ccggccacaa cctgggcgac
     4261 gtcgccggcc tggtgatggc ctggggcggc gcgctggccg gcaagctgcg gcccgatgcg
     4321 cgctacacct acggctggaa gcgcgcctcg atcctggccg cgttcgcgaa cgcgctgctg
     4381 ctgctggtgg cgatggggtc gctcgcctgg gaggcgatcg gccggctgag cgcgcccgag
     4441 ccgatccagg gcatcaccgt gatggtggtc gccggcatcg gcatcgtcgt caacgcgggc
     4501 acggcgctgc tgttcatgcg cggccgcgac gacgacatga acatccgcgg cgcctacctg
     4561 cacatggcca gcgacgcgct ggtgtcggcc ggcgtcgtgg tcgtcggcgc gctcgcgctg
     4621 tggaccggct gggcctggct cgacccggtc gcgagcctgt tgatcgcgtt cgtgatcgtg
     4681 gtcgggacct ggggcctgtt ccgccagtcg ctgcgcctga tgttcgatgc cgtgcccgaa
     4741 cacatcgacc tgcgcgcggt gcgcggcatg ctcgaggcgc tgcctggcgt ggcgcgggtg
     4801 cacgacctgc acgtctgggc caccggcacg accgacgtgg tgctgacggc gcacctggtg
     4861 atgccggccg ggcaccccga cgaccccttc ttcgagggcg cgacacacca gttgcgcgaa
     4921 cggttcaaga tcggccacgt caccttgcag gtcgtgcgcg agccgttcat ggcgctgtgc
     4981 gaaggcgcgc cggacgcgcc cgacctgcgg ccggcgcacc gccactgagg cagaagatcg
     5041 cgatggagtt caaggactac tacaagaccc tcggcgtggc gcgcgacgca tcgcccgacg
     5101 agatcaagag cgcgtaccgg aagctggcgc gcaaatacca cccggacgtc agcaaggagc
     5161 cgcaggccga ggcgcgcttc aaggacatcg gcgaagcgag cgaggtgctg cgcgaccccg
     5221 aaaagcgcgc cgcctacgac gccgccggtg cgcagtggga acgccagcag gccgcgggtg
     5281 ctgcgggccg gccgcaggac ttccagccgc cgcccggctg ggacaccggc ttcgagttca
     5341 gcggcggcga ggaagacctg ttcggcggcg ccgaccacag cgacttcttc gaggcgctgt
     5401 tcggccgggc cgcgcgtgcg ggaggtggca gggcgcgccg ccctgacgtg cagatgcccg
     5461 gccaggatca ccacgcgaag gtgatgatcg atctcgagga cgcgttccac ggcgcgcagc
     5521 gcagcatctc gctgcgcatg ccggcattgg acgatgaggg ccgcggcgtg ctgcgtgaac
     5581 gcacgctgga tgtcaacatc ccgaagggca tccgcgaagg ccagcacctg cggctggcgg
     5641 gccagggcgg cccgggtctg ggcaatgctc cggccggcga cctgttcctc gagatcgtct
     5701 tcaagccgca cccgcgctac cgggtcgacg gtcgcgatgt ctacgtcgac ctgccgctcg
     5761 cgccgtggga ggccgcgctc ggtgcggcgg tcggcgccgc gacgccggat ggcgaggtcg
     5821 agctgacgat cccgccgggg tcggccgccg gccgcaagct gcggctgaag ggccgcggca
     5881 ttccggggca gccgcctggc gatctctatg tggtgctggc gatcgcactg ccgccggcca
     5941 ccgacgcgaa ggcccgcgcc gcgtaccagg ccttcgcgca ggcgttcgag ttcgatccgc
     6001 gcgccccgaa gtgacagacc gaggatccct gccatgagcg agcccctgag cgccaccatc
     6061 gtcgaagacg acctgcagat gagtctggcc gaactggcgc gcgcctgccg cgcgcaggaa
     6121 gagcagatcc aggtctgggt cgtcgaaggc gtgctgcagc cca
//
LOCUS       DATMJB010000483         4199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_368473, whole genome shotgun sequence.
ACCESSION   DATMJB010000483 DATMJB010000000
VERSION     DATMJB010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368473"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1361
                     /locus_tag="VNU48_10305"
     CDS             87..1361
                     /locus_tag="VNU48_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HWI11711.1"
                     /translation="MTSALVISSPAAGAPDLPADLAAVGIHVLGAVLRRNMVHEAIRC
                     APDVVICYEAQVDPGLFGDMELLAATAARPVIVFTNDPDAEKIERALVAGVHAYVING
                     YAINRLRALVHVAQARFRHEQLLRGQLADVSHRFEERKLVDRAKGILMRALQMSEEDA
                     FRALRTASMHSKQRVGQVSQQVIAAAHYADAVNRAGKLRMLSQRLVKLCALRLAGPAS
                     ELGERQLAESIEQLDANIAALGKRLSKPTFGDLLDVVGQSAAQLRALVGATPALAQLG
                     ELDRLAELVLTQADQLTQHLETTGLVTTLHVINVSGRQRMLSQRFAKQALLGLLLRGD
                     EGLRAREAMANTALEFEQAMKYLKSTPLNAREIRELIEAAERAWATLTQAAEQVRSAA
                     GQRALGESSEAELAVFEQLTALYERNMQVLMG"
     gene            complement(1376..2173)
                     /locus_tag="VNU48_10310"
     CDS             complement(1376..2173)
                     /locus_tag="VNU48_10310"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI11712.1"
                     /translation="MQNFIEVQNAEMVFVTKKGRFHALHAIDLAVAKGEFVTLIGHSG
                     CGKSTLLNLIAGLTLPTGGVLLCDNREIAAPGPERGVVFQNHSLLPWLSCFENVHLAV
                     ERVFGATESSAQLRARTLAALELVNMGHAVSKRPHEISGGMKQRVGIARALAMEPKVL
                     LLDEPFGALDALTRAHLQDELLKIVARTQSTVVMVTHDVDEAVLLSDRIVMMTNGPAA
                     TIGEILSVDLPRPRNRVELAEDPRYTHLRKEVLDFLYTRHGHVEKAA"
     gene            complement(2187..3095)
                     /gene="ntrB"
                     /locus_tag="VNU48_10315"
     CDS             complement(2187..3095)
                     /gene="ntrB"
                     /locus_tag="VNU48_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HWI11713.1"
                     /translation="MVSAVFHTSLDTQARPQAAPAVAPVKPRPIVVAAVVPPERAQPG
                     FDWRGLAMKVLPPIVGIAVLIGIWAIATLNSTTFPTPGATFAEAVKVFADPFYRNGPN
                     DQGIGWNILFSLQRVAVGFGLAALVGIPAGFAIGRFEVLNHMVSPLISLLRPVSPLAW
                     LPIGLLVFKAANPAAIWTIFICSIWPMIINTAVGVQRVPSDYLNVARVLNLSEWKVVT
                     KILFPAVLPYMLTGVRLSVGTAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVKHILIAI
                     LVIGVVGLLLEWALISIAKRFSYDET"
     gene            complement(3108..>4199)
                     /locus_tag="VNU48_10320"
     CDS             complement(3108..>4199)
                     /locus_tag="VNU48_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HWI11714.1"
                     /translation="PLTDCASVVMASVLGFDKKYGVKIVPSKEASWAGVRDKLVNGEL
                     DMAHVLYGLIYGVHLGTAGPKKDMAVLMNLNNNGQAITLSKALADKGAVDGASLAKVM
                     AKEKREYTFAGTFPTGTHAMWIHYWLAASGINPLSGAKLITVPPPQMVANMRVGNMDG
                     FCVGEPWNHRAIIDGIGVTAITTQDIWKDHPEKTLGTTADFVKKNPNTCRAVMMAVLE
                     AGRWIDASLANKMKMAETVAEKSYINTGVDAINQRILGRYQNGLGKTWDDPNPMRFFN
                     DGATNFPYLSDGMWFLTQHKRWGLLKEHPDYLAVAKQVNQIDLYKQAAGALKISVPKE
                     PLRTSKLLDGVVWDGKDPKKYAESFAIKA"
BASE COUNT          648 a         1438 c         1414 g          699 t
ORIGIN      
        1 cggtgctggc cgcgaacttg cagtccacga ccacggcacg cagcccatcg cgccgccttc
       61 acccagcgaa agggcctgtt ttcccgatga cttccgcgct cgtcatttca tccccggccg
      121 ccggcgcgcc cgacctgccc gccgatctgg ccgcggtcgg cattcacgtg cttggtgccg
      181 tgctgcgccg caacatggtg catgaggcga tccgctgtgc gccggatgtc gtgatctgtt
      241 acgaggcgca ggtcgatccc ggcctgttcg gcgacatgga acttctggcc gccaccgccg
      301 cccgcccggt gatcgtgttc accaacgacc cggacgccga gaagatcgag cgcgccctgg
      361 tcgccggcgt gcacgcctat gtgatcaacg gctatgcgat caaccggctg cgcgcgctgg
      421 tgcacgtcgc gcaggcgcgc tttcgccacg agcagctgct gcgcggccag ctcgccgacg
      481 tgtcgcaccg cttcgaggag cgcaagctcg tcgatcgcgc gaagggcatc ctgatgcggg
      541 cgctgcagat gtccgaggaa gacgccttcc gcgcgcttcg caccgcgtcg atgcacagca
      601 agcagcgcgt cggccaggtc tcgcagcaag tcatcgccgc ggcccactac gccgacgcgg
      661 tgaaccgcgc cggcaagctg cgcatgctgt cgcagcgcct ggtcaagctg tgcgcgctgc
      721 gcctggccgg gccggcctcg gagctcggcg agcgccagct ggccgaatcg atcgagcagc
      781 tcgatgcgaa catcgccgcg ctcggcaagc gcctgtcgaa gccgaccttc ggcgacctgc
      841 tcgacgtggt cgggcagtcg gcggcgcagc tgcgcgcgct ggtgggtgcg acgccggcgc
      901 tcgcgcagct cggcgaactc gatcgcctcg cggagctggt gctgacgcag gccgaccagc
      961 tgacccagca cctcgaaacc accggcctcg tgacgacgct gcacgtgatc aacgtctcgg
     1021 gccggcagcg catgctgtcg cagcgcttcg ccaagcaggc gctgctcggc ctgctgctcc
     1081 gcggcgacga gggcctgcgc gcgcgcgagg cgatggccaa caccgcgctc gagttcgagc
     1141 aggcgatgaa atacctgaag tcgacgccgc tgaacgcgcg cgagattcgc gagctgatcg
     1201 aggcggccga gcgcgcctgg gcgacgctga cgcaggccgc cgagcaggtg cgcagcgccg
     1261 ccggccagcg cgcgctgggc gaatcgagcg aggccgagct cgccgtgttc gagcagctga
     1321 ccgcgctgta cgaacgcaac atgcaggtgc tgatgggctg aacgcggcgg ccgcgtcagg
     1381 ccgccttctc gacatgcccg tgacgcgtgt acaggaagtc gagcacctcc ttgcgcaggt
     1441 gcgtgtagcg cgggtcctcg gcgagctcga cgcggttgcg cgggcgcggc aggtcgacgc
     1501 tcaggatctc gccgatcgtc gcggccgggc cgttcgtcat catcacgatg cggtccgaca
     1561 gcagcaccgc ctcgtcgacg tcgtgcgtga ccatcacgac cgtgctctgg gtgcgcgcga
     1621 cgatcttcag cagctcgtcc tgcagatgcg cgcgggtcag cgcgtcgagc gcgccgaaag
     1681 gctcgtccag cagcagcacc ttcggctcca tcgccagggc gcgcgcgatg cccacgcgct
     1741 gcttcatgcc gccggagatc tcgtgcgggc gcttgctcac tgcgtgtccc atgttgacga
     1801 gttcgagtgc cgccagggtg cgcgcacgca gctgcgcgct cgattcggtt gcaccgaaga
     1861 cgcgctcgac cgccaggtgc acgttctcga agcagctcag ccacggcagc agcgagtggt
     1921 tctggaacac gacgccgcgt tcgggcccgg gcgcggcgat ctcgcggttg tcgcacagca
     1981 gcacgccgcc ggtgggcagc gtgagcccgg cgatcaggtt cagcaaggtc gatttgccgc
     2041 agcccgagtg gccgatcagc gtgacgaact cgcccttggc gaccgcgagg tcgatcgcgt
     2101 gcagcgcgtg gaagcgaccc ttcttcgtga cgaagaccat ctccgcgttc tgtacttcga
     2161 tgaagttctg catgtggaaa gctccttcag gtctcgtcgt agctgaaacg ctttgcgatc
     2221 gaaatcagcg cccattcgag cagcagcccg accacgccga tcaccaggat cgcgatcagg
     2281 atgtgcttga cgttcaggtt gttccactcg tcccagaccc agaagccgat gcccacgccg
     2341 ccggtcagca tctcggccgc gacgatcacc agccacgcgg tgcccacgct caggcgcaca
     2401 ccggtcagca tgtagggcag caccgcaggg aacaggatct tcgtgaccac cttccactcg
     2461 gacaggttca gcacgcgcgc gacattgagg tagtcgctcg gcacgcgctg cacgccgacc
     2521 gcggtgttga tgatcatcgg ccagatcgag cagatgaaga tcgtccagat cgcggccggg
     2581 ttcgcggcct tgaacaccag cagcccgatc ggcagccagg ccagcggcga gaccggccgc
     2641 agcaggctga tcagcggcga gaccatgtgg ttgagcacct cgaagcggcc gatcgcaaag
     2701 cccgccggaa tgcccaccag cgcggccagg ccgaagccga ccgcgacgcg ctgcagcgag
     2761 aacaggatgt tccagccgat gccctgatcg ttcgggccgt tgcggtagaa cggatcggcg
     2821 aacaccttga ccgcctcggc gaaggtcgcc cccggcgtcg gaaaggtcgt gctgttcagc
     2881 gtcgcgatcg cccagatgcc gatcagcacc gcgatgccga cgatcggcgg cagcaccttc
     2941 atcgccaggc cgcgccagtc gaagccgggt tgggcgcgct cgggcggaac gaccgcagcc
     3001 acgacgatcg gcctgggctt gaccggggcg accgccggcg ccgcctgggg gcgcgcctgg
     3061 gtgtcgagcg aggtgtggaa gacagcgctg accatggtgg acatccttca ggccttgatc
     3121 gcgaaactct cggcgtactt cttcgggtcc ttgccgtccc agaccacgcc gtccagcagc
     3181 ttgctggtgc gcagcggctc tttcggcacg ctgatcttca gcgcgccggc ggcctgcttg
     3241 tacagatcga tctggttcac ctgcttggcc accgcgaggt agtcggggtg ctccttcagc
     3301 aggccccagc gcttgtgctg ggtcaggaac cacatgccgt cggacaggta cgggaagttg
     3361 gtcgcgccgt cgttgaagaa gcgcatcggg ttcgggtcgt cccaggtctt gcccagcccg
     3421 ttctggtagc ggccgaggat gcgctggttg atcgcgtcga cgccggtgtt gatgtagctc
     3481 ttctcggcca cggtctcggc catcttcatc ttgttggcca ggctcgcgtc gatccagcgg
     3541 ccggcctcga gcacggccat catcacggcg cgacaggtgt tcgggttctt cttgacgaag
     3601 tcggcggtgg tgcccagcgt cttctcgggg tggtccttcc agatgtcctg cgtggtgatc
     3661 gccgtcacgc cgatgccgtc gatgatcgcg cggtggttcc agggctcgcc gacgcagaag
     3721 ccgtccatgt tgcccacgcg catgttcgcc accatctgcg gcggcggcac ggtgatgagc
     3781 ttggcgccgc tcagcgggtt gatgcccgac gcggcgagcc agtagtggat ccacatcgcg
     3841 tgcgtgccgg tcgggaaggt gccggcgaag gtgtactcgc gcttctcctt ggccatcacc
     3901 ttggccagcg acgcgccgtc gaccgcgccc ttgtcggcca gggccttcga cagcgtgatc
     3961 gcctggccgt tgttgttcag gttcatcagc acggccatgt ccttcttcgg gcctgccgtg
     4021 cccaggtgca cgccgtagat caggccgtac agcacgtgcg ccatgtcgag ctcgccgttg
     4081 acgagcttgt cgcgcacgcc ggcccacgag gcctctttgc tcggcacgat cttgacgccg
     4141 tacttcttgt cgaagcccag caccgaggcc atcacgaccg aggcgcaatc ggtcagcgg
//
LOCUS       DATMJB010000484         3389 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_138175, whole genome shotgun sequence.
ACCESSION   DATMJB010000484 DATMJB010000000
VERSION     DATMJB010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138175"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2783)
                     /locus_tag="VNU48_10325"
     CDS             complement(<1..2783)
                     /locus_tag="VNU48_10325"
                     /inference="COORDINATES: protein motif:HMM:NF040662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity factor TipJ family phage tail
                     protein"
                     /protein_id="HWI11715.1"
                     /translation="MIHAVVSHRPPAMRVSRNPLEAPAPMQVVRVGATIASLKPEATG
                     LLVCVVNGSPLPRLTARQRQTWVRKCAVDAIDGQKYLAKAARRELNWKTYRVRPGDVI
                     EWHDQPQEKQIAQIIIVIAAVIYGYYTGDWSTAFKFATYALAAVNFLLPPTVPDRNQE
                     EQGKDLFSTSLNGNQARLDQPIWRNFGRVKITPPFAAYPYYEYQDLDGDNLDNDQFYY
                     AVFAVGIGDHELESALIGRTPIGHFQDIQTATYLPPGTQPSIARCNVVSSTEVSTALE
                     LDSGRYVGGYAACQPRRRAGAVGVDVMCPQGLGKNGDPLTVSFQIEARDINEFGVAVT
                     SWYVIGNESHTASTNTPQRWSHKYAVGSIESTNPTTGDFEIVYGTGRRVEIRVIRTDL
                     KDTDSAARHALQWIGLRAYLDEPAPLDADTAHFEVVMRASEQLSSNSQRDFSLIVWPL
                     VRTWSPASLGSPSGWSAPMRTRNPAWALADLWTNPIWGEGLPDSRVDLQGLYEWSLKN
                     DTRQDRFDFSFSTNLSAWDAGQMIARAGRARVFRRYGVKTLARDELQELPMTALTPAN
                     TIADSMTINEKLPNRESPDGIVAQYTSNITWDDAFIECPAPGFTVTDPLDLRYDADLP
                     SMSNPVYKQYAGVTGAKHAEREGLYDAADMVLRNRTVTCTTEMMGVVTSYMMPVLFQP
                     DVAGYGQSGDVAFWDADTLVMGLTEKPEFGISGATFLTLVRDDGSVTDPVLVTPGPTE
                     WDITLPAAPDFDLVLDDGTRMRPKFVLGDMDLLVKISGISDAGTSDAPDGEQGSQLYG
                     ITAVVDDDRVHLADVHLLPGPGEIQDPVDPGVPVDGDDFYGIVTLRDHRIQAGQGVGG
                     GGYSASFTLFNDGTAQGHVEEGGPSSVDFPYSGEWLLVPVEVAQATQYEVYASINPFF
                     ASAFSGSALNTWLS"
     gene            complement(2780..3205)
                     /locus_tag="VNU48_10330"
     CDS             complement(2780..3205)
                     /locus_tag="VNU48_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11716.1"
                     /translation="MTSRSTHWAAALIGKPWRSAARGPREYSCWALAQRAVFLRQGLQ
                     LPDFDNEESIGAIRRGAETGGWRLVDGPPIEDDVVVMRNRLGQRHVGYMVIANGRLGL
                     LHADGHETSKGPVGCVVWQTLADATAGGYHDFEVWRHTT"
     gene            complement(3202..>3389)
                     /locus_tag="VNU48_10335"
     CDS             complement(3202..>3389)
                     /locus_tag="VNU48_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11717.1"
                     /translation="ERVYAADDTSGPAILPPTTLELDGALIKGTALSLKASFGNPNEV
                     NVPRRTFKRSAYPGLVR"
BASE COUNT          503 a         1103 c         1188 g          595 t
ORIGIN      
        1 gacagccacg tgttgagcgc tgaaccgctg aatgcgctgg cgaagaacgg gttgatcgac
       61 gcgtagacct cgtactgcgt agcctgcgcg acctcgacgg gaacgagtag ccactcgccg
      121 gagtacggga agtccaccga cgacgggccg ccttcctcga cgtggccttg cgccgtgccg
      181 tcgttgaaga gcgtgaacga agccgaatag ccgccgccgc cgaccccctg gccggcctgg
      241 atgcggtgat cgcgcagcgt gacgatgccg tagaagtcgt cgccgtcgac gggcacaccg
      301 gggtcaacgg gatcctgaat ctcgccgggt cccggcagca ggtgcacgtc ggcaaggtgc
      361 acgcggtcgt cgtcgacgac ggcggtgatg ccgtagagct gcgagccctg ctcgccgtcc
      421 ggtgcatcgc tggtgccggc gtcgctgatg ccgctgatct tcaccagcag gtccatgtcg
      481 ccgagaacga acttcggccg catgcgcgtg ccgtcgtcga gcacgagatc gaagtcggga
      541 gccgcgggca gcgtgatgtc ccactcggtc gggccgggcg tcactagcac ggggtcggtg
      601 accgagccgt cgtcgcgcac cagcgtcagg aatgtcgcgc cgctgatgcc gaactccggc
      661 ttctcggtca ggcccatcac cagcgtgtcg gcatcccaga acgcgacgtc gccgctctgt
      721 ccgtagccgg cgacgtcggg ttggaacagc acgggcatca tgtagctcgt gacgacgccc
      781 atcatctccg tcgtgcaggt caccgtgcgg ttgcgcagca ccatgtcggc ggcgtcgtac
      841 agaccctcgc gctcggcgtg cttggcgccg gtgacgccgg cgtactgctt gtagacgggg
      901 ttcgacatcg acggcaggtc ggcgtcgtag cgcaggtcca gtgggtcggt cacagtgaag
      961 cccggcgccg ggcactcgat gaacgcgtcg tcccacgtga tgttgcttgt gtactgcgcc
     1021 acgatgccgt ccggcgattc gcggttcggc agcttctcgt tgatcgtcat cgagtcggcg
     1081 atcgtgttcg ctggcgtcag cgcggtcatc ggaagctctt ggagctcgtc gcgcgccagc
     1141 gtcttcacgc cgtagcgccg gaagacacgg gcccggccgg cgcgcgcgat catctggccg
     1201 gcgtcccacg cgctcaagtt cgtcgagaac gagaagtcga agcgatcctg gcgggtgtcg
     1261 ttcttcaggc tccactcgta caggccttgc aggtcgacgc gcgaatccgg caggccttcg
     1321 ccccagatcg ggttcgtcca cagatcggcg agcgcccacg ctgggttgcg cgtacgcatt
     1381 ggcgcgctcc agccgctcgg gctgccaaga ctcgccggtg accacgtgcg caccagcggc
     1441 cagacgatca ggctgaagtc gcgctggctg ttcgaactca gctgctcgga ggcgcgcatc
     1501 acgacctcga agtgcgcggt gtcggcgtcg agcggcgcgg gctcgtcgag gtaggcgcgc
     1561 aggccgatcc actgcagcgc gtggcgcgcc gcgctgtcgg tgtccttcag gtcggtgcgg
     1621 atcactcgga tctcgacgcg cctgccggtg ccgtagacga tctcgaaatc gcctgtcgtc
     1681 gggttggtgc tctcaatgct gccaacggcg tacttgtggc tccagcgctg cggcgtgttc
     1741 gtgctcgccg tgtgcgactc gttgccgatc acgtaccacg acgtgactgc gacgccgaac
     1801 tcgttgatgt cacgcgcctc gatctggaag ctgacggtca gcgggtcgcc gttcttgccg
     1861 agaccctgcg ggcacatcac gtcgacaccg acggcgccgg cgcgacgccg cggctggcag
     1921 gcagcgtagc cgccgacgta gcggccgctg tcgagttcca gcgccgtcga cacctcggtc
     1981 gacgagacga cgttgcaacg cgcgatgctc ggctgcgtgc ccggcggcag gtacgtcgcc
     2041 gtctggatgt cctggaagtg accgatcggc gtgcgaccga tcagcgcgct ctcgagctcg
     2101 tggtcgccga tgccgacggc gaagacggcg tagtagaact ggtcgttgtc gaggttgtcg
     2161 ccgtcgaggt cctggtactc gtagtacggg taggcagcga acggcggcgt gatcttgacg
     2221 cggccaaagt tgcgccagat gggttgatcg aggcgcgctt ggttgccgtt gagcgaggtg
     2281 ctgaacaagt ccttgccctg ctcctcctga ttgcgatcgg gaaccgtcgg cggcagcagg
     2341 aagttgacgg cagccagcgc gtaggtcgcg aacttgaatg cggtggacca gtcgccggtg
     2401 tagtagccgt agatcacggc ggcgatcacg atgatgatct gcgcgatctg cttctcttgc
     2461 ggctggtcgt gccactcgat cacgtcgccc ggccggacgc ggtaggtctt ccagttcagt
     2521 tcgcgccgcg ccgccttggc gagatacttc tggccgtcga tcgcgtcgac cgcgcacttg
     2581 cgcacccacg tctggcgctg gcgtgccgtc agccgcggca gcggcgagcc gttgacgacg
     2641 cacaccagca agcccgtcgc ctcgggtttc agcgaggcga tcgtggcgcc gacgcgcacg
     2701 acctgcatcg gcgccggtgc ctcaagcggg ttgcgcgaga cacgcatcgc cggcgggcgg
     2761 tggctcacca cagcatggat catgtggtgt gtctccagac ttcgaagtcg tgatagccgc
     2821 cggcggtggc gtcggccagc gtttgccaaa cgacgcagcc gaccgggccc ttcgatgttt
     2881 cgtggccgtc ggcgtgaagc aggccgaggc ggccgttggc gatcaccatg tagccgacgt
     2941 gacgttggcc taaccggtta cgcatgacga cgacgtcgtc ctcgatcggc ggcccgtcca
     3001 ccaagcgcca gccgccggtc tcggcgccgc ggcggatggc accgatgctt tcctcgttgt
     3061 cgaagtccgg caactgcagg ccttggcgca ggaacaccgc gcgctgcgcg agcgcccagc
     3121 agctgtactc gcgcgggccg cgcgcagcgc tccgccaggg cttgccgatg agagcggcag
     3181 cccagtgtgt ggagcgcgag gtcaccggac taagcctggg taggctgagc gtttgaacgt
     3241 gcgccgcggc acgttcactt cgttcgggtt gccgaagctc gccttcagcg acagtgccgt
     3301 gcccttgatc agcgcgccgt cgagttcgag cgtcgtcggc ggcaggatgg ccgggccgct
     3361 ggtgtcatcg gccgcgtaga cgcgctcgg
//
LOCUS       DATMJB010000485         5429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_402736, whole genome shotgun sequence.
ACCESSION   DATMJB010000485 DATMJB010000000
VERSION     DATMJB010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5429
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402736"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..975
                     /gene="tam"
                     /locus_tag="VNU48_10340"
     CDS             196..975
                     /gene="tam"
                     /locus_tag="VNU48_10340"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HWI11718.1"
                     /translation="MTGDWNPELYRRFEDERTRPARELLARVTSAAPARVYDLGCGPG
                     NSTELLVERFPGAAVVGTDNSEAMLASARERLPQRRFELSDIARWRAPAPADLIYSNA
                     ALQWVGDHAALIPRLFAQLAPGGVLAIQMPDNRDEPTHRAMREVAGLAPWSALIGDAA
                     KLRTQVLGITDYYDLLTARGAEVDVWRTAYQHPMESPAKIVEWVRGTGLKPFVDPLPG
                     AERETFLAEYEARIARAYPKRADGKRLLQFPRLFIVARRPS"
     gene            1031..2032
                     /locus_tag="VNU48_10345"
     CDS             1031..2032
                     /locus_tag="VNU48_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HWI11719.1"
                     /translation="MTTHPRDALFDSADRAPLLPVCDHYCGIELRMRKSLALQAELGP
                     VFDITLDCEDGAPIGGEAEHAQLCAEVAMSAENRFGRVGARVHPVDHPSFAADVATLI
                     GRAGTRLAYLMLPKPRDLADVQRGCAEIDHQLRVHGIARALPVHALIETHGALRDVER
                     IAALPRIESLSFGLMDFVAAHRGAIPASAMSAEGQFRHPLVRRAKLEIAAACHGHAKT
                     PSHCVVTEFKDVAAIDAAATRASREFGYTRMWSIHPNQIRPIVEAFAPSAAEVELAWQ
                     IVEAAQHAQWAPIRHRDTLHDRASYRYYWQVLERAHRTGQPLPRTVHERFFEGGAAA"
     gene            2163..2756
                     /locus_tag="VNU48_10350"
     CDS             2163..2756
                     /locus_tag="VNU48_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11720.1"
                     /translation="MTIQSSALALAAALALCAAVPVATAQTATPKKPAAKKTAPAKKV
                     VEPPAPVAAGPEQIDAAGRVFYGVYDCEFKQTVDIVESSKYPAYVDVKHGKAAYLMKP
                     VLSSTGAIRLEDVRGETLMVQISSKSMLLNVKTAQRIVDDCVSPKQRELIEAARVAKA
                     AEVAAAAASATAAVSAAPAAAAATAAAPTSSAPTQAK"
     gene            2839..3084
                     /locus_tag="VNU48_10355"
     CDS             2839..3084
                     /locus_tag="VNU48_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWI11721.1"
                     /translation="MEATVAERGQITLPKAVRDALGLTKGTQLKVELDGSRIILRKNV
                     DDAISRARGKFKLDGFKDVDDAMRAIRGRAPGDPVDA"
     gene            3093..3497
                     /locus_tag="VNU48_10360"
     CDS             3093..3497
                     /locus_tag="VNU48_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWI11722.1"
                     /translation="MIAVDSSVLIEILIGAPQAEAAEGALREALAHGPVLVCDVVVAE
                     VCSSLRDGDQALQVLEDMGIRYGEVEARAAIRAGEMQRRYRARGGARSRTVPDFIVGA
                     HALLQCSALITRDAGFFRDYFKGLKLIVPTSA"
     gene            3551..>5429
                     /locus_tag="VNU48_10365"
     CDS             3551..>5429
                     /locus_tag="VNU48_10365"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HWI11723.1"
                     /translation="MLDAYRQHVAERAALGIPPLPLSAAQTAALIELLKAPPAGEEAA
                     LVELLTQRVPAGVDDAAKVKASYLAAVAHGSEASPLISRARATELLGTMLGGYNISPL
                     VDLLDDAVVGSVAAEGLKKTLLMFDQFHDVQHKATQGNANAQAVVRSWADAEWFTSRP
                     EVPASITVSIFKVAGEINTDDLSPAPDAWSRPDIPMHALAMHKNPRPGVVPEEDGKRG
                     PVKFIEELRARGHLVAYVGDVVGTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGG
                     KIAPIFYNTMEDSGALPIELDVSQMAMGDVVELRPYEGKALKNGQVIAEFKVKSEVLF
                     DEVRAGGRIPLIIGRGLTAKAREALGLPPSTLFRLPHNPADTHKGFSLAQKMVGKACG
                     LPVVNGQQTGVRPGTYCEPRMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHT
                     AAYPKPVDVKMHHELPEFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFP
                     VGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYAIKAG
                     LLTVEKKGKKNIFSGRILEIEGLPDLKAEQAFELSDASAERSAAGCTVHLNKEPIIEY
                     IN"
BASE COUNT          871 a         1985 c         1838 g          735 t
ORIGIN      
        1 gttcagccag gaacgcatca acgtgaccct ggccgaactg cagggcgagc aagacggcgt
       61 caagcacctg ctgtagcgcg tggttgcggg catgagttgc tgctcggttc gccgggtcgc
      121 cgcgccgggc cgccccaagc aaggcccgac cctcgcggag ggtcgcttgt cgtacccggc
      181 aagcggggtg ttgtcatgac cggcgactgg aaccccgagc tctaccgccg cttcgaggac
      241 gagcgcacgc gcccggcgcg cgagttgctc gcccgcgtga cgtccgccgc gccggcacgt
      301 gtctacgacc tgggctgcgg cccgggcaac tcgaccgagc tgctggtcga gcgcttcccc
      361 ggcgcggccg tcgtcggcac cgacaactcc gaggcgatgc tcgccagcgc acgcgagcgg
      421 ctgccgcagc ggcgcttcga gctcagcgac atcgcgcgct ggcgggcgcc ggcgcccgcc
      481 gacctgatct acagcaacgc cgcgctgcaa tgggtcggcg atcacgcggc gctgatcccg
      541 cggctgttcg cgcagctcgc gcccggcggc gtgctggcga tccagatgcc cgacaaccgc
      601 gacgagccga cgcaccgcgc gatgcgcgag gtcgccggcc tcgcgccttg gtcggcactg
      661 atcggcgacg ccgccaagct gcgcacgcag gtcctcggca tcaccgacta ctacgacctg
      721 ctgaccgccc gcggcgccga ggtcgatgtg tggcgcaccg cgtaccagca cccgatggag
      781 tcgcccgcga agatcgtcga atgggtgcgc ggcaccgggc tgaagccctt cgtcgacccg
      841 ctgcccggcg ccgagcgcga gaccttcctg gccgagtacg aggcgcgcat cgcgcgcgcc
      901 tacccgaagc gcgccgacgg caagcggctg ttgcagttcc cgcgcctgtt catcgtcgcg
      961 cgccggccgt catgagcccg ccgggccgcc ccaagggcga attccgaagt gcgcagcacg
     1021 gaggtaccca atgaccacgc acccgcgcga cgcgctgttc gacagcgccg accgcgcccc
     1081 gctgctgccg gtgtgcgacc actattgcgg catcgagctg cgcatgcgca agagcctggc
     1141 gctgcaggcc gagctcggcc cggtgttcga catcacgctc gactgcgagg acggcgcgcc
     1201 gatcggcggc gaggccgagc acgcccagct gtgcgcggaa gtggcgatgt cggccgagaa
     1261 ccgcttcggt cgtgtcggcg cgcgcgtgca cccggtcgat cacccgagct tcgcggccga
     1321 cgtggccacg ctgatcggtc gcgccggcac gcgcctggcc tatctgatgc tgcccaagcc
     1381 gcgcgacctc gccgacgtgc agcgcggctg cgccgagatc gaccaccagc tgcgtgtgca
     1441 cggcatcgcg cgcgcgctgc cggtgcacgc gctgatcgag acgcacggcg cattgcgcga
     1501 cgtcgagcgg atcgccgcgc tgccgcgcat cgagtcgctg tcgttcgggc tgatggattt
     1561 cgtcgcggcg caccgcggcg cgatccccgc cagtgcgatg agcgccgagg gtcagttcag
     1621 gcacccgctg gtgcggcgcg ccaagctcga gatcgccgcg gcctgccacg gccacgccaa
     1681 gacgccgtcg cactgcgtcg tcaccgaatt caaggacgtc gccgcgatcg atgccgccgc
     1741 cacgcgcgca tcgcgcgagt tcggctacac gcgcatgtgg agcatccacc cgaatcagat
     1801 ccggccgatc gtcgaggcct tcgcgccgag cgcggccgag gtggaactgg cgtggcagat
     1861 cgtcgaggcc gcgcagcacg cgcaatgggc gccgatccgg caccgcgaca cgctgcacga
     1921 ccgcgccagc taccgctact actggcaggt gctcgaacgc gcgcaccgca ccggccagcc
     1981 gctgccgcgg acggtgcacg agcgcttctt cgagggcggc gcggccgcat gaccgactcc
     2041 gcgggccgcg ccgcatggtg ttgcatttcg caaccgccgg gcggcccacc gcgtgttgca
     2101 agtcacaacg tcgacgcacg ccgccaccgc acaatgcggc cacctcagct tccggccaca
     2161 ccatgacgat tcaatcctcg gccctcgcgc ttgccgccgc cctcgccttg tgcgccgccg
     2221 tgccggtcgc gaccgcgcaa accgcgacac cgaagaagcc cgccgcgaag aaaacggcgc
     2281 cggccaagaa ggtcgtcgag ccgccggcgc cggtggccgc cggccccgag caaatcgatg
     2341 cggcggggcg cgtcttctac ggcgtctacg actgcgagtt caagcagacc gtcgacatcg
     2401 tcgagagcag caagtacccc gcctacgtgg acgtgaagca cggcaaggcc gcgtacctga
     2461 tgaagccggt gctgtcgtcg accggcgcga tccgcctcga ggacgtgcgc ggtgagacgc
     2521 tgatggtgca gatctcgagc aagtcgatgc tgctgaacgt gaagaccgcg cagcgcatcg
     2581 tcgacgactg cgtcagcccg aagcagcgcg agctgatcga ggccgcgcgg gtcgcgaagg
     2641 ccgccgaagt ggcggcagcc gccgcctcgg ccacggcggc ggtgtcggca gcgcccgcgg
     2701 ccgccgcggc gacggcagcg gccccgacca gcagcgcccc gacccaggcg aagtgaagcc
     2761 gacggcgccg ctgagcgcct tgcggtacgc caggatcagt atgtataatt catgcatact
     2821 tcgatccagg agcactgcat ggaagccacc gtcgccgaac gcggtcagat cacgctgccg
     2881 aaggccgtgc gcgacgcgct cggcctgacc aagggcacac agctcaaggt cgagctcgac
     2941 ggcagccgca tcatcctgcg caagaacgtc gacgacgcga tctcgcgcgc ccgcggcaag
     3001 ttcaagctcg acgggttcaa ggacgtcgac gacgcgatgc gcgcgatccg cggtcgcgcc
     3061 ccgggcgatc cggtcgacgc ctgagtcttg cgatgatcgc cgtcgactcc tccgtgctga
     3121 tcgagatcct gatcggcgct ccgcaggccg aggccgccga aggcgcgctg cgcgaggccc
     3181 tcgcgcacgg cccggtgctg gtctgcgacg tggtcgtcgc cgaggtctgc agttcgctgc
     3241 gcgacggcga ccaggccttg caagtgctcg aggacatggg catccgctac ggcgaggtcg
     3301 aggcccgggc cgcgatccgt gccggcgaga tgcaacgccg ctaccgcgcc cgcggcggcg
     3361 cgcgctcgcg caccgtgccc gacttcatcg tcggggcaca tgccttgctt cagtgcagtg
     3421 cactgatcac gcgcgacgcc ggtttcttcc gcgactattt caagggcctg aagctcatcg
     3481 tgccaacgtc ggcatgactc gcccgcccca acccgttccc accgtttgca gccatctcaa
     3541 ggagctcccc atgcttgacg cctaccgcca acacgtcgcc gaacgcgccg ccctcggcat
     3601 cccgccgctg ccgctgtcgg ccgcgcagac tgccgcgctg atcgaactgc tgaaggcgcc
     3661 gcccgcgggc gaggaagccg cgttggtcga gctgctcacg cagcgcgtgc cggccggcgt
     3721 cgacgatgcg gccaaggtca aggcgagcta cctcgcggcg gtcgcccacg gcagcgaggc
     3781 ctcgccgctg atctcgcgcg cacgcgccac cgaactgctg ggcaccatgc tcggcggcta
     3841 caacatcagc ccgctggtcg acctgctcga cgatgcggtc gtcggctcgg tcgcggccga
     3901 gggcctgaag aagacgctgc tgatgttcga ccagttccac gacgtgcagc acaaggcgac
     3961 ccagggcaac gcgaacgcgc aggccgtggt gcgcagctgg gccgacgccg agtggttcac
     4021 gtcgcgcccc gaagtgccgg cctcgatcac cgtcagcatc ttcaaggtcg ccggcgagat
     4081 caacaccgac gacctctcgc cggcccccga tgcctggagc cgccccgaca tcccgatgca
     4141 cgcgctggca atgcacaaga acccgcgccc cggcgtcgtg cccgaggaag acggcaagcg
     4201 cggcccggtc aagttcatcg aggagttgcg cgcgcgcggc catctcgtcg cctacgtcgg
     4261 cgacgtggtc ggcaccggct cgagccgcaa gtccgcgacc aactcggtgc tgtggttcac
     4321 cggcgaagac atcccgttcg tgccgaacaa gcgcttcggc ggcgtctgcc tgggcggcaa
     4381 gatcgcgccg atcttctaca acacgatgga agactcgggc gcgctgccga tcgagctcga
     4441 cgtgagccag atggcgatgg gcgacgtcgt cgagctgcgc ccgtacgagg gcaaggcgct
     4501 gaagaacggc caggtcatcg ccgagttcaa ggtcaagagc gaggtgctgt tcgacgaggt
     4561 gcgcgcgggc gggcgcatcc cgctgatcat cggccgcggc ctgaccgcga aggcgcgcga
     4621 ggcgctcggc ctgccgccga gcaccctctt ccgcctgccg cacaacccgg ccgacacgca
     4681 caagggcttc agcctggcgc agaagatggt cggcaaggcc tgcggcctgc ccgtcgtgaa
     4741 cggccagcag accggcgtgc gccccggcac ctactgcgaa ccgcgcatga ccagcgtcgg
     4801 ctcgcaggac accaccggcc cgatgacgcg cgacgagctc aaagacctcg cctgcctggg
     4861 cttttccgcc gacctggtga tgcagagctt ctgccacacc gccgcctacc cgaagccggt
     4921 cgacgtgaag atgcaccacg agctgcccga gttcatcagc acgcgcggcg gcgtctcgct
     4981 gcggcccggc gacggcgtga tccacagctg gctgaaccgc ctgctgctgc ccgacacggt
     5041 gggcaccggc ggcgacagcc acacgcgctt ccccgtaggc atcagcttcc cggccggctc
     5101 gggcctggtc gcgttcgccg ccgccaccgg cgtgatgccg ctggacatgc ccgagagcgt
     5161 gctggtgcgc ttcaagggca agatgcagcc gggcgtgacc ttgcgcgacc tggtcaatgc
     5221 gatcccgctg tacgcgatca aggccgggtt gctgacggtc gagaagaagg gcaagaagaa
     5281 catcttcagc ggccgcatcc tcgagatcga aggcctgccc gacctgaagg ctgaacaagc
     5341 cttcgagctg agcgacgctt cggccgagcg ctcggccgcc ggctgcacgg ttcacctgaa
     5401 caaggaaccg atcatcgagt acatcaaca
//
LOCUS       DATMJB010000486         2857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_616656, whole genome shotgun sequence.
ACCESSION   DATMJB010000486 DATMJB010000000
VERSION     DATMJB010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616656"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VNU48_10370"
     CDS             <1..466
                     /locus_tag="VNU48_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWI11724.1"
                     /translation="VLVPFLLSTAVFTRLSVIDLSLPTQSNEKLEKLSANNLQLEIVV
                     RPEALEVGDRLGGLIQRIPATATGPDVRALQTLVQQIKTKFPDKLDATVLAEPATSYD
                     TLVQVMDAVRAGHMAQQGKLVKAELFPNISIGDAPIVHAAVTVAAAPARGVR"
     gene            463..957
                     /locus_tag="VNU48_10375"
     CDS             463..957
                     /locus_tag="VNU48_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWI11725.1"
                     /translation="MKLTPLQKRAERKSRNQTMVDMNLVSLIDVFTILIFFLLSNSGG
                     VETLPSPKAVHLPESVAEVSPRDTVVVVVSGSEIIVDGRRIAMVAEVLGTDGDLIAPL
                     KAELDLQSNRQVIRKENEAQSKRLTIMGDKDIPYRLLRKIMFTSARANFSDVSFAVRQ
                     RFET"
     gene            954..2120
                     /locus_tag="VNU48_10380"
     CDS             954..2120
                     /locus_tag="VNU48_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HWI11726.1"
                     /translation="MSAAAMTMTPMVSFRVPVLPWAISADDERRFKRIARVVLITAAV
                     VCIVLPWLPVLKPDRAQPQELPPRLAKLILERELPPPPPPPPMARPKVEAPPAVAQRE
                     VEATRPDPVKASPRRPDAPAKVALNKEAVVPEARVPLPNKPPGEIDAARRKAAGVGLL
                     AMKDDLAELHGAPVAVQLAPVKQGPGVGSGVGVGVGAGNEAGVPTRALITSNATGGSG
                     GINTASYSRNTGGGGLAGRSTTLVEGLAGGGGGGGAGGGGARGKGDGTGSGYGGGGGG
                     TGANGGGTLQRGGSGKASRSIEEVKLVFERNKGSIYAIYNRALREEPSLQGKVVLKLT
                     IAPSGGVVECRIESSELRTPELEAKLLARIRQFDFGAKDVDQMIVTWPVDFLPS"
     gene            complement(2137..>2857)
                     /locus_tag="VNU48_10385"
     CDS             complement(2137..>2857)
                     /locus_tag="VNU48_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI11727.1"
                     /translation="TELDIPNKLQPPGAAHWLGTDSLGRDIVSLLIVGSRNSIAVGLI
                     AVGIGMVFGVALGLLAAARRGWVEELVMRFSDFAFAFPALLSAIMLTAVYGPGLTTAI
                     VAIGIFNVPVFARITRASANAVWTREFVLAARACGKGAWSITWRHVLPNIAAVLIVQA
                     TIQFAVAILAEAALSYLGLGTQPPMPSWGRMLNEGQNMMYQSWLLAVWPGLAIVLSVL
                     GLNLMGDGLRDLLDPRLARAR"
BASE COUNT          472 a          966 c         1039 g          380 t
ORIGIN      
        1 ggtgctggtg ccgttcctgc tctcgaccgc ggtgttcacg cgcctgtcgg tgatcgacct
       61 gagcctgccg acgcagtcga acgagaagct cgagaagctg agcgcgaaca acctgcaact
      121 cgagatcgtc gtgcggcccg aggcgctcga ggtcggcgac cgcctgggcg ggctgatcca
      181 gcgcatcccg gcgaccgcca ccggccccga tgtgcgcgcg ctgcagacgc tggtgcaaca
      241 gatcaagacc aagttccccg acaagctcga cgccaccgtg ctcgccgagc ccgcgacctc
      301 gtacgacacg ctggtgcagg tgatggacgc ggtgcgtgcc ggccacatgg cgcagcaggg
      361 caagctggtc aaggccgagc tgttccccaa catctcgatt ggcgacgcgc cgatcgtgca
      421 tgcagcggtc actgtggccg cggcgccggc gaggggggtg cgatgaagct gacgccgctg
      481 caaaagcgcg ccgagcgcaa gagccgcaac cagacaatgg tcgacatgaa cctcgtctcg
      541 ctgatcgacg tgttcacgat cctgatcttc ttcctgctgt cgaactccgg cggcgtcgag
      601 accctgccga gcccgaaggc ggtgcacctg cccgagtcgg tcgccgaagt cagcccgcgc
      661 gacaccgtcg tggtcgtcgt cagcggctcg gagatcatcg ttgacgggcg caggatcgcg
      721 atggtggccg aggtgctcgg caccgacggc gacctgatcg cgccgctgaa ggccgagctc
      781 gatctgcaat cgaaccgcca ggtgatccgc aaagagaacg aagcccagag caagcgcctg
      841 acgatcatgg gcgacaagga cattccgtat cgcctgctgc gcaagatcat gttcaccagc
      901 gcgcgcgcga acttcagcga cgtgtcgttc gcggtgcggc agaggttcga gacatgagcg
      961 ccgcggcgat gacgatgacg ccgatggtgt cgttccgggt gccggtgctg ccgtgggcga
     1021 tttcggccga cgacgagcgc cgcttcaagc gcatcgcccg ggtggtgctg atcaccgcgg
     1081 ccgtggtctg catcgtgctg ccgtggctgc cggtgctgaa gcccgatcgc gcgcagccgc
     1141 aggagctgcc gccgcgcctg gcgaagctga tcctcgagag ggagctgccg ccaccaccac
     1201 cgccgccacc gatggcacgg ccgaaggtcg aagcgccgcc agcggtcgcg caacgcgaag
     1261 tcgaggctac gcggcccgat ccggtgaagg catcgccgcg ccggcccgat gcgccggcca
     1321 aggtcgcgct gaacaaggaa gccgtcgtgc ccgaggcgcg cgtgccgctg ccgaacaagc
     1381 cgccgggcga gatcgacgcg gcgcggcgca aggccgcggg cgtcggcctg ctcgcgatga
     1441 aggacgacct ggccgagctg catggcgcgc cggtcgcggt gcagctggcg ccggtgaagc
     1501 aggggccggg tgtgggcagc ggcgtcggtg tcggcgtcgg cgcgggcaat gaagccggcg
     1561 tgccgacgcg cgcgctgatc acctcgaacg cgaccggcgg cagcggcggc atcaacaccg
     1621 cgagctacag ccgcaacacc ggcggcggag gcctggcggg ccgctccacc acgctggtgg
     1681 aaggcctggc cggcggcggt ggtggcggtg gcgcgggagg cggtggtgca cgcggcaagg
     1741 gcgacggcac cggcagcggt tacggcggag gcggtggcgg caccggcgcg aatggcggcg
     1801 gcacgctgca gcgcggcggc agcggcaagg cctcgcgctc gatcgaggag gtgaagctcg
     1861 tcttcgagcg caacaagggc tcgatctacg cgatctacaa ccgcgcgctg cgtgaggagc
     1921 cgagcctgca gggcaaggtc gtgctgaagc tgacgatcgc accctcgggc ggcgtggtcg
     1981 agtgccggat cgaatcgagc gagctgcgca cgcccgagct cgaggcgaag ctgctcgcgc
     2041 gcatccggca gttcgacttc ggtgcgaagg atgtcgacca gatgatcgtg acctggccgg
     2101 tcgacttcct gccctcgtga gcctggttga tgagagtcag cgggcccgcg cgagccgcgg
     2161 gtcgagcagg tcgcgcaggc catcgcccat caggttcagc ccgagcaccg acagaacgat
     2221 cgccagcccc ggccacaccg ccagcagcca cgactggtac atcatgttct ggccctcgtt
     2281 gagcatgcgg ccccagctcg gcatcggcgg ctgcgtgccc aggccgaggt agctcagcgc
     2341 cgcctcggcc aggatcgcga ccgcgaactg gatcgtcgcc tgcacgatca gcaccgccgc
     2401 gatgttcggc agcacgtggc gccaggtgat gctccacgcg cccttgccgc aggcgcgcgc
     2461 ggcgagcacg aactcgcgcg tccagacggc gttcgcgctg gcgcgcgtga tgcgtgcgaa
     2521 caccggcacg ttgaagatgc cgatcgcgac gatcgcggtc gtcagcccgg ggccgtagac
     2581 cgcggtcagc atgatcgcgg acagcagtgc cgggaacgcg aacgcgaagt ccgagaagcg
     2641 catcaccagc tcctcgaccc agccgcgccg cgccgcggcg agcagcccga gcgcgacgcc
     2701 gaagaccatg ccgatgccca ccgcgatcag cccgaccgcg atcgagttgc ggctgccgac
     2761 gatcagcagc gagacgatgt cgcggcccag gctgtcggtg ccgagccagt gcgccgcgcc
     2821 cggcggctgc agcttgttcg ggatgtcgag ctcggtg
//
LOCUS       DATMJB010000487         8295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_381230, whole genome shotgun sequence.
ACCESSION   DATMJB010000487 DATMJB010000000
VERSION     DATMJB010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8295
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381230"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..113
                     /locus_tag="VNU48_10390"
     CDS             <1..113
                     /locus_tag="VNU48_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI11728.1"
                     /translation="RGTPEEIAAAVAFLASDDSSYITGIDLAVDGGMAQV"
     gene            216..848
                     /locus_tag="VNU48_10395"
     CDS             216..848
                     /locus_tag="VNU48_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003576355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI11729.1"
                     /translation="MYNQAKLSELIRFARVDAGSTVIDVYPGDGDWTRLFSDVVGPEG
                     RVFSFVPAEVAHFKNDPVGHMRTLAKEPGRENVEVVSADLVAMPEVTQPADVLWLHLF
                     YHDLHTALIQAKGATAAAFNRAVYERLKPGGSYVIVDHAATAGAGTSDSQSLHRIDPA
                     SVREEVQAAGFVLEAQSALLAKKDDPHSVKVFDPSVKGETDRFAYRFVKA"
     gene            complement(965..1498)
                     /locus_tag="VNU48_10400"
     CDS             complement(965..1498)
                     /locus_tag="VNU48_10400"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="HWI11730.1"
                     /translation="MASPRKGSDRARSKAAARRQAPAQSWQAWRIADGRFDPFSPVGA
                     SLVGGRWNSPGLGVIYASRSYAGAMLECLAHAGIGRVPRTHVAVEIAIAEALAVERHD
                     ESSLPAGWDHADLRVARAFGDTWIRELRTAVLVVPSVVARREGNVLINPRHPHFSGII
                     AGAPEPVVWDARLFAPH"
     gene            complement(1482..1916)
                     /locus_tag="VNU48_10405"
     CDS             complement(1482..1916)
                     /locus_tag="VNU48_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008344738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HWI11731.1"
                     /translation="MYAAVVEPSKIAQVLGLRRKVTSIEQLDQQVEAGLPKASLGAVA
                     RHVYGTSPDAAALMQRVIPSATFHRRGEDLKPQEGERVERLARVIATAEHVWDDVDDA
                     RAFLSTGHAMLGGKRPIEVALTELGARRVENLLWSLFHGVAA"
     gene            complement(2061..2735)
                     /locus_tag="VNU48_10410"
     CDS             complement(2061..2735)
                     /locus_tag="VNU48_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11732.1"
                     /translation="MSFLEELKRQASDLQAAKSIDQSALARNTALAEAAATEASRYLL
                     ELAKQLEVIRPVPKASYQFDSKTRIEGLPMQKFRFDSRRKRVRDLDLTDYMSFGCVIR
                     GLEPVVLKKNFVPDIEKLEARLWQSGTTVQAEPVRDPENGRLVEMRYEFTPEVWVGLK
                     IVPNHELGLLRFRMNNIDGLETVECEFAPHEVGQRRLDELARWWLGHPHQFLDRASEI
                     RRVEPR"
     gene            2823..3662
                     /locus_tag="VNU48_10415"
     CDS             2823..3662
                     /locus_tag="VNU48_10415"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HWI11733.1"
                     /translation="MTCRRFLLMRALAAVPVLLAAACAVPPPALDDSALPRLRAARFI
                     LLGEVHDNASQHRLRAALLAELLADGRPTTVVFEQLGRAEDGAITAAPRDAEAIADAG
                     RLDRKGWGWPLHKPLLDAAIAASARIAGGNLEAPQVRAIVRTGLPAVPADLRPLLEGP
                     GWGARRQQALEREIDSGHCHALPASQWPAMALAQRARDAALAQALLSATAAGGRAVLI
                     AGNGHVRLDLGVPHYLRAAGVPAAQTVAVGYLEEDEGDAPYDLVRVTGRMPRDDPCAA
                     FAR"
     gene            complement(3675..4559)
                     /locus_tag="VNU48_10420"
     CDS             complement(3675..4559)
                     /locus_tag="VNU48_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI11734.1"
                     /translation="MQISANGIALEVEDHGSPAGEPLVLIMGLGMQLVAWHPEFVAML
                     VARGFRVIRFDNRDSGLSQRFDHLGVPNLALDSLRHAMGMRVSAPYTVADMADDTAGL
                     LAALGIQAAHICGASMGGMIAQQLAVRHPRRVKSLTLIMTSSGSRRLPGPTLKVRSAL
                     ISRPADPNNLQSVIEHYVKLYRLIGSPAYPAPEAYLQEHLGQAVRRSYRPAGTARQMV
                     AIAADGNRSKLLAQIRVPTQIIHGQADPLVPVAAGSDLAAKIAGAELDLIAGMGHDLP
                     TELWPRFVSGIASAAARA"
     gene            complement(4562..4696)
                     /locus_tag="VNU48_10425"
     CDS             complement(4562..4696)
                     /locus_tag="VNU48_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11735.1"
                     /translation="MNATGDTPAKMAEQLGRERIARQTWRSLPEHGRPPVEPTGAERF
                     "
     gene            complement(4698..5522)
                     /locus_tag="VNU48_10430"
     CDS             complement(4698..5522)
                     /locus_tag="VNU48_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI11736.1"
                     /translation="MPRLPRSHAPDTTPSSDGELRPPNAFLLMLEGRAFWELGAMLAA
                     TPWLRRLPRGDGHPVLVFPGMGANDFTTVPLRNFLDGLGYATQAWGQGFNFGPRDGVL
                     KRCAADVRALAGKHGRPVSLVGWSLGGLYAREMAKELPDHTRCVITLGTPFAGHPRAT
                     NAWRIFEMLSGQKAHDPAMIARLRTPPPVPTTSIYSRTDGIVAWQCSLNEPGALTENI
                     EVHASHVGMGMNPLALYAVADRLAQPAGQWRHFDASDGVRRWFFQSAPANPSTKAA"
     gene            complement(5554..7254)
                     /locus_tag="VNU48_10435"
     CDS             complement(5554..7254)
                     /locus_tag="VNU48_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HWI11737.1"
                     /translation="MKQLPGLDASFLHLETPQMPMHVGALHNFELPASYRGDFLADMR
                     RHMAARLVLAPALRQKLVKMPLNLSNPTWIDAEPDLEAHIVGITLPKSKSGSDQAELE
                     AQVGRLHPVLLDRSRPLWKFHVFDGLADGPGGTKRYAMYTQLHHAAVDGQAAVALGQA
                     ILDLGPEGREVDQQRSGKVRRELGMADMLRGALGNQLGLVVDAVKAIPGTVGVLSQMA
                     KQKVGDTAGGTIAALVARLKGQGAGAKGKSVSNLALAPRTRLNVTVSETRAFAAISLP
                     LAAINATRRKHKASLNDAVLMICSGALRRHFLKHGPLPRKSMVAGVPISTRAKGDTAS
                     NNQATMTIVSLGTHIADPDKRLAHVLAATAAMKANMGAMKSLMPTDFPSLGVPWLMEG
                     LSALYEKARVADRLPAIANVTISNVPGPTVPLYMAGAKMLTNYPTSIVVHGVALNITV
                     QTYNESLEFGLIACGKAMPEIAELAQHVQAAFDEFQALPATVEAPVATPAPKVVAKKA
                     APVRKPGAKKVAAKRPATKKTAAAAAAKKLATKPSAKTVPKVTPRARASAPKKKATRR
                     "
     gene            complement(7355..8234)
                     /locus_tag="VNU48_10440"
                     /pseudo
     CDS             complement(7355..8234)
                     /locus_tag="VNU48_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010109606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
BASE COUNT         1231 a         2880 c         2853 g         1331 t
ORIGIN      
        1 gcagaggaac gcccgaggaa atcgcggcag cagtcgcgtt tctcgcttcg gacgatagtt
       61 cttacatcac cggtatagat ctggccgtcg atgggggcat ggcgcaggtc tgaccccggc
      121 gtggacctgt gcccatcgac acccaagaat ggcacgtgga tcgcccccgc gcacgccgcg
      181 cgctcgttga atcctctttt gcaaaggaga tctcgatgta caaccaagcc aagctatctg
      241 agttgatccg gttcgcacga gtcgatgcgg gctccaccgt tatcgacgtt tatccaggcg
      301 acggcgactg gacccgtctc ttctccgacg tcgtgggacc cgaagggcga gtcttcagct
      361 tcgtgccggc tgaagtcgcc cacttcaaga acgatccggt cggccacatg cggacgcttg
      421 cgaaggagcc aggccgagag aacgtcgaag tcgtctccgc ggacctcgtg gcgatgccgg
      481 aggttaccca accagcggat gtcctgtggc tgcacctgtt ctatcacgat ctgcacaccg
      541 cgctgatcca ggccaagggc gcgacggcgg cagccttcaa ccgagccgtc tacgagcggc
      601 tcaagcccgg tgggtcttac gtcatcgtcg accacgccgc caccgcaggg gccggcacga
      661 gcgacagcca gtcgctgcat cggatcgacc ccgcgtccgt tcgcgaggag gtgcaggcag
      721 ccggcttcgt gctcgaggcg caaagcgcct tgctcgcgaa gaaggacgat ccgcactcgg
      781 tcaaggtctt cgatccctcg gtcaagggtg agactgaccg cttcgcctat cggttcgtga
      841 aggcctgacc gggcgcatgt atccgacaaa gaagtttcct gctgtcggcg atacgcgaac
      901 cggccgggct tgccctgcac tccgtctgtt ccgccgactg cttcgacaag tgagacgatg
      961 aacatcagtg cggggcgaac agccgagcat cccagaccac cggttccggt gcgccggcga
     1021 tgatgccgct gaagtgagga tgccgaggat tgatgagaac gttgccttcc ctgcgtgcca
     1081 ccaccgaagg tacgaccagc accgccgttc gcagctcgcg aatccaggtg tcgccaaagg
     1141 ctcttgccac ccgcagatcc gcatgatccc aaccagcggg cagactgctc tcgtcgtgtc
     1201 gctcgacggc gagcgcctcc gcgatggcga tctcgacagc tacatgggtg cgcggcaccc
     1261 ttccgatgcc ggcatgggcg agacactcca gcatcgcgcc cgcatacgag cggctggcgt
     1321 agatgacacc caggcccggt gaattccaac gcccaccgac aagcgaggca cccactgggc
     1381 tgaatgggtc gaaccggccg tcggcgatgc gccaagcctg ccaggattgc gctggtgcct
     1441 gtcttctggc cgccgccttc gatctcgccc tgtccgagcc cttacgcggc gacgccatgg
     1501 aaaagcgacc agagcaggtt ctcgacgcgg cgcgcgccga gttctgtcag cgccacctcg
     1561 atcggccgct tgccgcccag catggcgtgg ccggtggaca ggaaggcccg cgcgtcgtcg
     1621 acgtcgtccc agacatgttc tgcagtggcg atcacgcgcg ccagacgctc cacccgctct
     1681 ccctcctgcg gcttcaagtc ctcacccctg cggtggaacg tggccgacgg gatgacccgc
     1741 tgcatcaggg ctgccgcatc gggcgaggtg ccgtacacat ggcgtgccac ggcgcccagc
     1801 gaggccttgg gcagacccgc ttcgacctgc tgatccagtt gctcgatgga agtgaccttg
     1861 cggcgcaagc ccagcacctg ggcgatcttg ctcggttcga cgacggctgc gtacatgaac
     1921 tggctcccaa ttgatagatt gaagtctatc agatgaagaa tggctgcctg gggtcgacgg
     1981 ccagaacccg gcacggcgcc atcggtccga ccagccgagc gcggtggcac caaacgcctc
     2041 cgttctgcct cacgggctcg ctacctcggt tcgacccggc ggatttcact ggcgcgatcg
     2101 aggaactgat gcgggtgccc gagccaccag cgcgccaact cgtcgagccg acgctgcccg
     2161 acctcgtgcg gcgcaaactc gcactcgacg gtttccaggc catcgatgtt gttcatcctg
     2221 aaccgcaaca ggcccaattc atgattgggc acgattttca ggccgaccca cacttccggc
     2281 gtgaactcgt atctcatctc gaccagacgc ccattctctg gatcgcgcac gggctcggct
     2341 tgtaccgttg tgccgctttg ccaaagtcgg gcttcgagct tctcgatgtc agggacgaag
     2401 ttcttcttca acacgacagg ttcgaggccg cggatcacgc atccaaagct catgtagtcg
     2461 gtgagatcca gatcacgcac ccgcttgcgt cgcgaatcaa agcgaaactt ctgcatcggc
     2521 aggccctcta tgcgggtctt gctgtcgaat tggtagctcg ccttggggac ggggcgaatc
     2581 acttccagct gcttcgccaa ttccagcaga tagcggcttg cttcggtggc agcagcttca
     2641 gccagcgcag tgttgcgcgc aagggcactc tggtcgatgc tctttgcggc ttgcagatcg
     2701 ctggcctgtc tcttcagttc ttcaaggaag ctcatcgctc gacgtcaggt tcgtgttcgc
     2761 tcgggttgcg cgcgaccgcc attgtgccca cgtcggcccg gaccagccgc taagctgggt
     2821 gcatgacctg tcgccgattc ctgttgatgc gcgcgttggc tgccgtgccc gtgctgctcg
     2881 cggccgcgtg cgccgtgccg ccgccggcgc tcgatgattc ggcgctgccc cgcttgcgcg
     2941 cagcccgctt cattctgctc ggtgaggtcc acgacaacgc gagccagcat cggctgcgcg
     3001 ccgcgctgct cgccgaactg ctcgccgatg gccggccgac gacggtcgtg ttcgaacaac
     3061 tggggcgcgc cgaagacggc gcgatcaccg cggcgccgcg cgatgccgaa gcgatcgccg
     3121 acgccggccg gctcgatcgc aagggctggg gctggccgct gcacaagccg ctgctcgatg
     3181 ccgcgatcgc ggcctcggcg cgcatcgcgg gcggcaacct cgaagcgccg caagtgcgtg
     3241 cgatcgtgcg caccggcctt ccggccgtcc ccgccgatct gcgtccgctg ctcgaaggtc
     3301 cgggctgggg cgcgcgccgg cagcaggcgc tcgagcgcga gatcgatagc ggccactgcc
     3361 acgcgctgcc tgcttcccag tggccggcga tggcactggc ccagcgcgcg cgcgacgccg
     3421 cactggcgca ggcgctgctg agcgccaccg cggccggcgg ccgcgccgtg ctgatcgccg
     3481 gcaacggcca cgtgcggctc gacctgggcg tgccgcacta cttgcgcgcg gccggcgtgc
     3541 cggcagcgca gaccgtcgcg gtcggctatc tcgaagagga cgagggcgac gcgccctacg
     3601 acctggtgcg agtcaccggc cgcatgccgc gcgacgaccc ctgcgcggcg ttcgccaggt
     3661 gagcgcggtc gacgtcaggc gcgcgccgcc gccgacgcga tgccgctgac gaagcgcggc
     3721 cacagctcgg tcggcaggtc gtggcccatg ccggcgatca ggtcgagctc ggcgccggcg
     3781 atcttcgccg ccagatcgct gccggccgcc accggcacca gcgggtcggc ctggccatgg
     3841 atgatctggg tcggcaccct gatctgcgcc aacagcttcg agcggttgcc gtcggcggcg
     3901 atcgcgacca tctggcgcgc cgtgccggcg ggccggtacg agcgccgcac cgcctggccc
     3961 aggtgctcct gcaggtaggc ctcgggcgcc gggtaggcgg ggctgccgat cagccggtac
     4021 agcttgacgt agtgctcgat cacgctctgt aggttgttcg ggtcggccgg gcgcgagatc
     4081 agcgcactgc gcaccttcag ggtcggcccg ggcaggcggc gcgagccgct gctggtcatg
     4141 atcagcgtca ggctcttgac gcgccgcggg tgccgcaccg cgagctgctg cgcgatcatg
     4201 ccgcccatcg aggcgccgca gatgtgcgcg gcctgaatgc ccagcgccgc cagcagcccc
     4261 gcggtgtcgt cggccatgtc ggccaccgtg tagggtgcgc tgaccctcat gcccatcgca
     4321 tgccgcagcg agtcgagcgc caggttgggc acgccgaggt ggtcgaaacg ctgcgacagg
     4381 ccgctgtcgc ggttgtcgaa gcggatcacg cgaaagccgc gcgccaccag catcgcgacg
     4441 aactccgggt gccaggccac gagctgcatg cccaggccca tgatcagcac gagcggttcg
     4501 ccggcgggcg aaccatggtc ctcgacttcg agggcgatgc cgttggcgga gatctgcatg
     4561 gtcagaagcg ctccgcgccg gtcggctcga ccggcgggcg accgtgctcg ggcaagctgc
     4621 gccaggtctg gcgcgcgatg cgctcgcgcc cgagttgttc cgccatcttg gccggcgtgt
     4681 cgcccgttgc gttcacttca ggccgctttc gtcgaggggt tggcgggggc actttggaag
     4741 aaccagcgcc gcacgccatc gctcgcatcg aagtggcgcc actgccccgc cggttgcgcg
     4801 agccgatcgg cgaccgcgta gagcgcgagc gggttcatgc ccatgccgac gtgactggcg
     4861 tggacctcga tgttctcggt gagcgcgccc ggttcgttca ggctgcactg ccaggcgacg
     4921 atgccgtcgg tgcgcgagta gatcgaggtc gtcggcaccg gcggtggcgt gcgcaggcgc
     4981 gcgatcatgg ccgggtcgtg cgccttctgg ccgctgagca tctcgaagat gcgccaggcg
     5041 ttggtcgcgc gcgggtggcc ggcgaacggc gtgcccagcg tgatcacgca gcgcgtgtgg
     5101 tcgggcagtt ccttcgccat ctcgcgcgca tacaggccgc ccaggctcca gccgaccagg
     5161 ctgaccgggc ggccgtgctt cccggccagc gcccgcacat cggcggcgca acgcttgagc
     5221 acgccgtcgc gcgggccgaa gttgaagccc tggccccagg cctgggtcgc gtagcccagg
     5281 ccgtcgagga agttgcgcag cggcacggtc gtgaagtcgt tcgcgcccat gcccgggaac
     5341 accagcaccg ggtgcccgtc gcccctgggc aggcgccgca gccacggtgt ggcggcgagc
     5401 attgcgccca gttcccagaa ggcccggcct tcgagcatca gcaggaaggc attcggtgga
     5461 cgcaactcgc cgtcgctcga aggcgtcgtg tcgggagcgt gggatcgggg cagtcggggc
     5521 atgccgctcg cgcgcggtgg gaatcggcgc atgttagcgg cgggtggcct tcttctttgg
     5581 agcacttgcc ctagcgcggg gcgtcacttt tgggactgtt ttggcggacg gtttcgtcgc
     5641 gagcttcttg gccgccgccg cagcggcagt cttcttcgtg gcaggcctct tcgccgcgac
     5701 cttcttcgcc cccggtttgc gaaccggggc ggccttcttc gcgacgacct ttggagcagg
     5761 cgtcgcaacc ggtgcttcga cggtcgcggg cagtgcctgg aattcgtcga acgccgcctg
     5821 cacatgctgg gcgagttcgg cgatctccgg catcgccttg ccgcacgcga tcaacccgaa
     5881 ctcgagcgac tcgttgtagg tctgcaccgt gatgttcagc gccaccccgt gcacgacgat
     5941 ggaggtcggg tagttggtca gcatcttcgc cccggccatg tacagcggca cggtcgggcc
     6001 gggcacgttc gagatcgtca cgttggcgat cgccggcagc cggtcggcga cgcgcgcctt
     6061 ctcgtacagc gcgctcagcc cctccatcag ccagggcacg cccagcgacg ggaagtcggt
     6121 cggcatcagg ctcttcatcg cgcccatgtt ggccttcatc gccgcggtgg cggcgagcac
     6181 gtgggcgagg cgcttgtccg ggtcggcgat gtgcgtgccc aggctgacga tcgtcatcgt
     6241 cgcctggttg ttggacgcgg tgtcgccctt cgcgcgggtc gagatcggca cgcccgccac
     6301 catcgacttg cgcggaagcg gcccgtgctt caggaagtgc cggcgcagcg caccgctgca
     6361 gatcatcagc accgcgtcgt tcaggctggc cttgtgcttg cgccgcgtcg cgttgatcgc
     6421 cgccagcggc aggctgatcg ccgcgaacgc acgcgtctcg gacaccgtga cgttcagccg
     6481 cgtgcgcggc gcgagcgcga ggttgctgac gctcttgccc ttggcgccgg caccctgccc
     6541 cttcaggcgc gccacgagcg cggcgatcgt gccgccggcc gtgtcgccga ccttttgctt
     6601 cgccatctgc gagagcacgc cgacggtgcc cgggatcgcc ttcaccgcgt cgacgaccag
     6661 cccgagctgg ttgccgagcg cgccgcgcag catgtcggcc atgccgagct cgcgtcggac
     6721 cttgccgctg cgctgctggt cgacctcgcg cccttccggc ccgaggtcga ggatcgcctg
     6781 gccgagcgcg accgcggcct ggccgtcgac cgccgcgtgg tgcagctggg tgtacatcgc
     6841 atagcgcttg gtgccgccgg ggccgtcggc caggccgtcg aagacgtgga acttccacag
     6901 tggccggctg cggtcgagca gcaccgggtg cagacggccg acctgggcct cgagctcagc
     6961 ctggtcgctg ccggatttcg atttcggcag cgtgatgccg acgatgtgcg cctcgagatc
     7021 gggctcggcg tcgatccagg tcgggttgct caggttcagc ggcatcttca cgagcttctg
     7081 gcgcagcgcc ggtgccagca ccaggcgcgc cgccatgtgg cgccgcatgt cggcgaggaa
     7141 gtcgccgcgg tagctggccg gcagctcgaa gttgtgcagc gcgccgacat gcatcggcat
     7201 ctgcggcgtt tcgaggtgca ggaagctggc gtcgagaccg gggagctgtt tcatgcggcg
     7261 atccggagca gggatggcgt cgatgctagt gcgccggccc cggcgcgcgc tgaggggaag
     7321 ccctgtcgcg cgcgcgtcag cgcgtcagcg cctctcagcg cggcgcctga cgcagtgcca
     7381 gtgcttcgtt cgccgcgagc gcgcccagca ccagcgcgcc gccgccgagc acgtgggcgg
     7441 caggcgcctc gcccgcgccc agccaggccc acgcgacgcc gaagatgacc tcgagcagcg
     7501 ccagcagcga ggcctcgggc gcgctcaggt ggcgcgccgc ggcgaccgac atcaggcagg
     7561 gcagcgcgag ctgcacgacc ccgagcaggg cgagcaggcc gaggtcgtgc gccgacgccg
     7621 cgaacggcag cgcgaagggc aaggtcagca ccgccgagag gaccgcgccg atcagcacgg
     7681 ccggcagcag atcgacgtcg gcgtggcggc gggtgtgctg gatcagcgtc cagttggccg
     7741 ccgcggcgat cggcacgccg agcgcgacca gcgtgccggc gagctgccgc tggtcggcgg
     7801 caccgacctc gcgcccgtac atccagccga tgccgatgcc ggcgacgacg atcgcggccc
     7861 aggtgcgcgc ggccagccga tggccgagga acacgcgcgc gatcagcgcg gtcagcagcg
     7921 gcgcgatcgc catcgttcac cagcacgttc gcgacggtgg tcagcgtgat cgcgaccatg
     7981 aacgcggtga acatcacgca ccagcacagg cccgagcacc acaggaccgc gccgccgtcg
     8041 cgccacgcgc gcgccagcac ggcccggccg cgcagccagg gcaacagtag cgcgagcgcc
     8101 agcaccgtga acgcgctgcg ccagaacgtg acctcgaagc tgcgcgccgc ctcgagctgg
     8161 cgcgtgacga cgccggcgat gctccacatc agcgtgacgg ccagcatcgt cgcgaccgcc
     8221 cggccgtggg tcatgacaac ccctcgccct cggagtaccg cggtcgatcc cccgggggga
     8281 tcgggccttg cttgg
//
LOCUS       DATMJB010000488         4296 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_515393, whole genome shotgun sequence.
ACCESSION   DATMJB010000488 DATMJB010000000
VERSION     DATMJB010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515393"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="VNU48_10445"
     CDS             <1..1070
                     /locus_tag="VNU48_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWI11738.1"
                     /translation="VSPAEAFGAVPGTASPEALARSAHKRRLADQRAALQLCERQFAE
                     AARDCKQLTELVGSKPLEAGAQAQQLSKALVDKMLGDQELCIRLLSDGAGDKAALHAM
                     NVAVISLLMGRTFGLSEADMLDLGVGAMLHDVGKLELPERVRHREDHFSGSELRFYEE
                     HVAHSVNHARKMSLTPGAMLVIAQHHEHADGSGFPLRLNTDRMSTGSRIVSLVNRYDN
                     LCNPNVASRALTPHESLSLLFAQGKTKFDTAILGAFIRMMGVYPAGSTVQLTDDRYAM
                     VVGVNSTRPLKPRVLVHEPKVPRDEALIVDLESTQGIGIRRSIRPAQLPVPSLEYLAP
                     RPRIAYFFEPLAAANDAALAA"
     gene            1081..3177
                     /locus_tag="VNU48_10450"
     CDS             1081..3177
                     /locus_tag="VNU48_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWI11739.1"
                     /translation="MNRDLVVHAPETAARAAWGGLIEAMLDAVWLVDALTLRIVAANH
                     AAGELMAMAPAELIGKDVLELAATPEDLCFWGEVAQGLADRIESDTLVTDANGATRAV
                     TRRVACVRGDDTAALFVVVLHDRSAQLRTERELEGTCADLRATLESTHDGILVTDLAG
                     RIRNFNRRFASLWQIPTELLERGEDDAVLEWMRRSVADPAAYMRRLAAIDGATMLQAS
                     DVLRLHSGTALERETLPQCLNGRPIGRVYAFRDITEKLEANRRIETLSFTDALTGLPN
                     RRLLADRIEVALAQAKRDATPFALLFLNLDRFNHINETLGRGFGDRVLLDVADRISGC
                     VREVDTVARLGGDEFVVLANQADARGAEATAWRVMEALKRPFTQGGMSFTVTASVGIA
                     LHPSDGASMDALMRRSDAAMREVKHAGRAAFRFHRTRPDGDDGQSRSRMRLDHAMRQA
                     LAQDRFRLHYQPQVDLNSGAVVGAEALIRWRDPELGEVSPVEFIPVAEESGFIVALGD
                     WVLKRAVAQAAAWADAGLWVPVSVNVSALQFQQPGFVDGVATVLRDAGLAPQWLELEL
                     TESILVQDAQDAMLRLRALAELGVQLAIDDFGTGYSSLAYLKRVPVGRLKIDRSFVGG
                     LPADESDAAIVRAIIQMGRALRLQIVAEGVENEAQRAFLQTAGCDLFQGFLYAPALAA
                     SAFEARVGRAGAGPAL"
     gene            3274..>4296
                     /locus_tag="VNU48_10455"
     CDS             3274..>4296
                     /locus_tag="VNU48_10455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI11740.1"
                     /translation="MKVMDSLLGRKLEGLTLPLALVLPDGHRIGAADAAITIRLKQLS
                     PLAHIAAGEVGRVAQDYVEDKLDFDGSVRDLMVIAAQMLDSDPRHGGDTSATLNWWRE
                     IRLNARSRARHQVHADAQQVQFHYDVSDAFYELWLDPMRLYSCAYFREPSLTLAQAQQ
                     AKLDHICRKLMLRPGERFLDIGAGWGGLLMWAAEHYGVRAHGITLSRNQHAHVNQLIE
                     ARGLAGRVTMELLDYRELPEGTPYDKIASVGMFEHVGRALMPAYFAKIHRLLKPGGLL
                     LNHGITAGGTRNRQLGAGMIEVRARCATVPQTERSRLDFPVSALDVALMGALPRLPWW
                     RRPRSGD"
BASE COUNT          621 a         1566 c         1514 g          595 t
ORIGIN      
        1 ccgtgtcgcc cgccgaggcg ttcggcgccg tgccgggcac tgcctccccc gaggcgctcg
       61 cgcgcagcgc gcacaagcgc cggctggccg accagcgcgc tgcgctgcaa ctgtgcgagc
      121 gccagttcgc cgaggcggcg cgcgactgca agcagctcac cgaactggtc ggcagcaagc
      181 cgctcgaggc cggtgcccag gcgcagcagc tgtcgaaggc gctggtggac aagatgctgg
      241 gcgaccagga gctgtgcatc cgcctgctgt cggacggcgc cggcgacaag gccgcgctgc
      301 atgccatgaa cgtggccgtg atctcgctgc tgatgggccg caccttcggc ctgtccgagg
      361 ccgacatgct cgacctgggc gtcggcgcga tgctgcacga cgtcggcaag ctcgagctgc
      421 ccgagcgcgt gcgccatcgc gaagaccatt tcagcggctc cgaactgcgc ttctacgagg
      481 agcacgtcgc gcacagcgtc aaccacgccc gcaagatgag cctcacgccg ggtgcgatgc
      541 tggtgatcgc gcagcaccac gagcatgccg acggcagcgg cttcccgctg cgcctgaaca
      601 cggaccgcat gagcacgggc tcgcgcatcg tgtcgctggt gaaccgctac gacaacctgt
      661 gcaacccgaa cgtcgcgtcg cgcgcgctca cgccgcacga atcgctgtcg ctgctgttcg
      721 cgcagggcaa gaccaagttc gacaccgcga tcctcggcgc cttcatccgc atgatgggcg
      781 tctacccggc cggctcgacg gtgcagctca ccgacgaccg ctacgcgatg gtcgtcggcg
      841 tcaactcgac ccggccgctg aagccgcgcg tgctggtgca cgagccgaag gtgccgcgcg
      901 acgaggcgct gatcgtcgac ctcgagtcga cccagggcat cggcatccgg cgcagcatcc
      961 ggccggcgca gctgccggtg ccctcgctcg agtacctggc gccgcgcccg cgcatcgcgt
     1021 acttcttcga gccgctcgcg gccgcgaacg acgccgcgct ggcggcctga tcggggcgcc
     1081 gtgaaccgcg acctcgtggt ccacgctccc gagacggcgg cacgcgccgc ctggggcggg
     1141 ctgatcgagg cgatgctcga cgcggtctgg ctcgtcgacg cgctcacgct gcgcatcgtc
     1201 gccgcgaacc atgccgccgg cgaactgatg gcgatggcgc cggccgaact gatcggcaag
     1261 gacgtgctgg aactcgccgc gacgcccgag gacctgtgct tctggggcga ggtggcgcag
     1321 gggctggccg accgcatcga gtccgacacg ctggtcaccg atgcgaacgg cgcgacgcgc
     1381 gcggtcacgc gccgtgtcgc ttgcgtccgg ggcgacgaca cggcggcgct gttcgtcgtc
     1441 gtgctgcacg accgcagcgc gcagctgcgc accgagcgcg agctcgaagg cacgtgcgcc
     1501 gacctgcgcg cgacgctcga atcgacccac gacggcatcc tcgtcaccga cctggccggg
     1561 cggatccgca acttcaaccg gcgctttgcc agcctgtggc agatcccgac cgagctgctc
     1621 gagcgcggcg aagacgatgc cgtgctcgaa tggatgcgcc gcagcgtggc cgaccccgcc
     1681 gcctacatgc gacgcctggc cgcgatcgac ggcgcgacga tgctgcaggc gagcgacgtg
     1741 ctgcgcctgc actcgggcac cgcgctcgaa cgcgagacgc tgccgcaatg cctgaacggc
     1801 cggccgatcg ggcgcgtcta cgcgttccgc gacatcaccg agaagctcga ggcgaaccgg
     1861 cgcatcgaga ccctgtcgtt caccgacgcg ctgaccgggt tgccgaaccg gcgcctgctg
     1921 gccgaccgca tcgaggtcgc gctcgcgcag gccaagcgcg acgccacgcc gttcgcgctg
     1981 ctgttcctga acctggaccg cttcaaccac atcaacgaga cgctgggccg cggcttcggc
     2041 gaccgcgtgc tgctcgacgt cgccgaccgc atcagcggct gcgtgcgcga ggtcgacacg
     2101 gtcgcgcgcc tcggcggcga cgagttcgtc gtgcttgcca accaggccga cgcgcgcggc
     2161 gccgaggcca ccgcgtggcg cgtgatggag gcgctgaagc ggccgttcac gcagggcggc
     2221 atgagcttca ccgtcaccgc cagcgtcggc atcgcgctgc acccgagcga cggcgcgagc
     2281 atggacgcgc tgatgcggcg cagcgacgcg gcgatgcgcg aggtcaagca cgccgggcgc
     2341 gccgcgttcc gcttccaccg cacccgcccc gacggcgacg acggacagtc gcgctcgcgc
     2401 atgcgcctgg accacgcgat gcgccaggcg ctggcacagg accgcttccg cctgcactac
     2461 cagccgcagg tcgacctgaa cagcggcgcg gtggtcggcg ccgaggcgct gatccgctgg
     2521 cgtgatcccg aactcggcga ggtctcgccg gtcgagttca ttccggtggc cgaggaaagc
     2581 ggcttcatcg tcgcgctcgg cgactgggtg ctcaagcgcg cggtcgcgca ggccgcggcc
     2641 tgggccgacg cggggctgtg ggtgccggtg tcggtgaacg tctcggcgct gcagttccag
     2701 cagcccggct tcgtcgacgg cgtggccacg gtgctgcgcg acgccggcct ggcgccgcag
     2761 tggctggagc tggagctgac cgaatcgatc ctggtccagg acgcgcagga cgccatgctg
     2821 cggctgcgcg cgctcgccga actcggtgtg cagctcgcga tcgacgactt cggcaccggc
     2881 tattcgagcc tggcctacct gaagcgcgtg ccggtcgggc gcctgaagat cgaccgcagc
     2941 ttcgtcggcg ggctgccggc cgacgagagc gacgcggcga tcgtgcgcgc catcatccag
     3001 atgggccggg cactgcgcct gcagatcgtc gccgagggcg tcgagaacga ggctcagcgc
     3061 gccttcctgc agacggccgg ctgcgacctg ttccagggtt tcctgtacgc gccggcgctg
     3121 gccgcgtcgg ccttcgaggc gcgcgtcggg cgcgccggcg ccggcccggc gctctgaggg
     3181 ccccccaggt ccgggctgcc acgtttgctc gagaaaaacc cagcgagcag cgatcgccct
     3241 tcttttgtac agtcggacac cggaggtccg acgatgaagg tgatggactc gttgctgggg
     3301 cgcaagctgg agggcctgac cctgccgctc gcgctggtgt tgcccgacgg tcaccggatc
     3361 ggcgccgcgg acgcggcgat cacgatccgg ctcaagcagc tgtcgccgct ggcgcacatc
     3421 gccgccggcg aggtcggccg ggtcgcacag gactacgtcg aggacaagct cgacttcgac
     3481 ggcagcgtgc gcgacctgat ggtgatcgcc gcgcagatgc tcgacagcga cccgcgccac
     3541 ggcggcgaca ccagcgccac gctgaactgg tggcgcgaga tccgcctgaa cgcgcgctcg
     3601 cgcgcgcgcc accaggtgca cgccgacgcg cagcaggtcc agttccacta cgacgtgtcg
     3661 gacgcgttct acgaactctg gctcgacccg atgcgcctgt actcgtgcgc ctacttccgc
     3721 gagccgtcgc tgacgctcgc gcaggcccag caggccaagc tcgaccacat ctgccgcaag
     3781 ctgatgctcc ggcccggcga gcgctttctc gacatcggcg ccggctgggg cgggttgctg
     3841 atgtgggccg ccgagcacta cggcgtgcgc gcgcacggca tcacgctgtc gcgcaaccag
     3901 cacgcgcacg tgaaccagct gatcgaggcg cgtggcctgg ccggccgggt cacgatggag
     3961 ctgctcgact accgcgagct gcccgagggg acgccctacg acaagatcgc gtcggtgggc
     4021 atgttcgagc acgtcgggcg cgcgctgatg ccggcctact tcgcgaagat tcatcgcctg
     4081 ctcaagcccg gcggcctgtt gctgaaccac ggcatcacgg ccggcggcac gcgcaaccgc
     4141 cagctcggcg ccggcatgat cgaggtcagg gccaggtgcg cgacggtccc gcagaccgag
     4201 cgctcccgcc tcgacttccc cgtctccgcc ctcgacgtgg cgctgatggg cgccctgccc
     4261 cgtctgccct ggtggcggcg cccccgctcc ggcgac
//
LOCUS       DATMJB010000489         6271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_848623, whole genome shotgun sequence.
ACCESSION   DATMJB010000489 DATMJB010000000
VERSION     DATMJB010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848623"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VNU48_10460"
     CDS             <1..281
                     /locus_tag="VNU48_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008162812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI11741.1"
                     /translation="LSIGRALLTHPELIILDEATEGLAPLIVAEIWRVIGAIRATGIS
                     TLIVDRNYRAVLAHTDRAVVLEKGLVVAAGASAELARDHDALARFLGV"
     gene            426..728
                     /locus_tag="VNU48_10465"
     CDS             426..728
                     /locus_tag="VNU48_10465"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWI11742.1"
                     /translation="MILEVADIRIPPGKQAEFDAAIQHGIASAISKAQGFRGFKVNRG
                     VESPERYILMIYWDTLENHTVDFRQGPLFPQWRAIVGPFFAAPPVVEHFELVAKSD"
     gene            739..2103
                     /locus_tag="VNU48_10470"
     CDS             739..2103
                     /locus_tag="VNU48_10470"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWI11743.1"
                     /translation="MNPSWLRRAAAAIAASSAMLGFAADAPLQIAPVAFSERVLANGL
                     QVIAVPSSASPTVSVQVWYHVGSKDDPPGRSGFAHLFEHLMFKSTAHLKAEQFDRLTE
                     DVGGSNNAFTGDDVTGYTDLVPSNHLEVLLWAEAERMSNLNVVQANFVSERAVVEEEY
                     RERVLAAPYGRFGNAIAPAAYQVHPYKRPSIGSIADLDAATLADVVAFHANHYRPDNA
                     TLVVAGDFDAAQLDAWVDKYFARVPRPSAPRPRVSVDEPAWAADRSVTVTAPQVPLPA
                     IALVWLAPPVTSADAPALQIAAALLGSGESSRLNQALVYRQRIAGQIGFDADLRAGPG
                     LLTATAIAASGKPLTAVARALLAEVLRLAAQPVGAAELAKVKTQLLTAALLARQTPEG
                     LASAVADAAVLQGGAGHVNTDLVALQNVSAADVQRVLQRYVARAHKVSLQYVQAATQE
                     GGAK"
     gene            2100..3560
                     /locus_tag="VNU48_10475"
     CDS             2100..3560
                     /locus_tag="VNU48_10475"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWI11744.1"
                     /translation="MNPSLARSIGALLLSLAALAPIARAQGFDTPPQPGAPRPLTVRA
                     PVEQRLPNGLRVVLAERRGVQLVTAQLVVLSGSEVDPPQRAGLASMTAGLLTKGTRRH
                     GATALAQAAESLGGALDSGAGWHQSEVAITVTVPKLDAALGLVSEVVQQPVFAPAELD
                     RLRAQALDELKVAYTQPATLAGLAAQRLLFGSGAYGHPAGGTPASLARIARADLLALH
                     AARYRPDNAVLVLAGDLHAADALRLAQRHFGAWKARGSAAAPAPLPVGAALAQSTAVI
                     DLPASGQAAVVVAAALPPLGADRATAAVMNAVLGGGYSSRLNQEIRIRRGLSYGAGSA
                     LDAFADGGSLRAVVQTKNESAAEVLGLVQSELDRLAAAPVGADELAARKATLIGDFSR
                     SVETTAGLGAAVKALIVAGRPLAELGLRIDALSAVGADAVQRYAAAQLGTNGRRVVVA
                     GDAARFAQALKATTPGLSVIASDKLDLDLGSALSAP"
     gene            3712..3969
                     /locus_tag="VNU48_10480"
     CDS             3712..3969
                     /locus_tag="VNU48_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11745.1"
                     /translation="MATRRPTSPTASDTTQKLRQTQAEYVAARPAGEPGVKPMMSSSK
                     MYVCRRCQANFKTGDGKPDARLPGLGKCNKCLAAATAALAA"
     gene            complement(4154..4408)
                     /locus_tag="VNU48_10485"
     CDS             complement(4154..4408)
                     /locus_tag="VNU48_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11746.1"
                     /translation="MHFDERKIGEYRAYAGALEGPMGDGYIAALVVLREQGTVGAAHE
                     ILRDESLACGHRWPSADAALAYAMTKAHEAIRKQSPMPIC"
     gene            4741..5811
                     /locus_tag="VNU48_10490"
     CDS             4741..5811
                     /locus_tag="VNU48_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HWI11747.1"
                     /translation="MSFRTLSDTIARALGFTGLLWRWGRVKGVTGALRTALAKVGIAP
                     GSAPRTTPRRAAATPTDAVRFLSRSFTNAAGTRAYKLYVPASYRGTAVPLLMMLHGCK
                     QNPDDFAAGTRMNELAEAHGFLVAYPAQSERANGANCWNWFRSGDQARDGGEPSILAG
                     IVAAIAADYAIDRRRVFVAGLSAGAAMAVILGRTHPEVFTAVGAHSGLPYRAAQGVAS
                     AFAAMHGGLAQAIAGWPPLRAADAAARAAAVPAVPTIVFHGDHDPTVAASNSDAIVAQ
                     ALAAPQASALLESRRLEPASAVSRGHTNTAYTTPDGRTLVEQWTLHGGAHAWSGGSPE
                     GSYTEAQGPDASAEMVRFFLAQ"
     gene            6047..>6271
                     /locus_tag="VNU48_10495"
     CDS             6047..>6271
                     /locus_tag="VNU48_10495"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWI11748.1"
                     /translation="MIPRDILVVAAARTAIGSFGGSLKDVPLRQLATTAVRGALQRSG
                     AAAAQVGQVVMGNVIPTEPADAYLSRVAAVD"
BASE COUNT          890 a         2317 c         2204 g          860 t
ORIGIN      
        1 tgctctcgat cggccgcgcg ctgctgacgc atcccgagct gatcatcctc gacgaagcca
       61 ccgaagggct ggcgccgctg atcgtcgccg agatctggcg cgtgatcggc gcgatccgcg
      121 ccaccggcat cagcacgctg atcgtcgacc gcaactaccg tgcggtgctg gcgcacaccg
      181 accgcgcggt ggtgctcgag aaggggctgg tcgtcgcggc cggcgcatcg gccgaactgg
      241 cgcgcgatca cgacgcgctc gcgcgcttcc tcggcgtcta gaaatgaggc ccccggcgac
      301 ggcgtaccgt cgccacccgc ccggcgggtc gggccttgct tggggcggcc cggcgcggcg
      361 gcccgcgccc accgcgcggt acttgtagac tggcggccct gtcaccgtcc cgcgaaggac
      421 cgcgcatgat tctcgaagtc gccgacatcc gcatcccgcc cggcaagcag gccgagttcg
      481 acgccgcgat ccagcacggc atcgccagcg ccatctccaa ggcccagggc tttcgcggct
      541 tcaaggtcaa ccgcggcgtc gaatcgcccg agcgctacat cctgatgatc tactgggaca
      601 cgctcgagaa ccacaccgtc gacttccgcc agggcccgct gttcccgcag tggcgcgcga
      661 tcgtcgggcc gttcttcgcg gcgccgccgg tggtcgagca tttcgagctg gtcgccaagt
      721 cggactgagg tcggacgcgt gaatccgagc tggctgcggc gcgccgccgc agcgatcgcc
      781 gcatcgagcg cgatgctcgg cttcgcggcc gatgcgccgc tgcagatcgc gccggtcgcg
      841 ttcagcgagc gcgtgctggc caacggcctg caggtgatcg cagtgcccag cagcgccagc
      901 ccgacggtca gcgtgcaggt ctggtaccac gtcggctcga aggacgaccc gcccggtcgc
      961 tcgggcttcg cgcacctgtt cgagcacctg atgttcaaga gcacggcgca cctgaaggcc
     1021 gagcagttcg accggctcac cgaagacgtc ggcggctcga acaatgcgtt caccggcgac
     1081 gacgtgaccg gctacaccga cctggtgccg tcgaaccacc tcgaggtgct gctgtgggcc
     1141 gaggccgagc gcatgtcgaa cctgaacgtg gtgcaggcga atttcgtctc cgagcgcgcg
     1201 gtcgtcgaag aggaataccg cgagcgcgtg ctggccgcgc cttacggccg cttcggcaac
     1261 gcgatcgcgc cggccgcgta ccaggtgcac ccgtacaagc gcccgagcat cggcagcatc
     1321 gccgacctcg acgcggcgac gctggccgat gtcgtcgcct tccacgccaa ccactaccgc
     1381 cccgacaacg ccacgctggt ggtggccggc gacttcgacg ccgcgcagct cgacgcctgg
     1441 gtcgacaagt acttcgcgcg cgtgccgcgt ccgagcgcgc cgcggccgcg cgtcagcgtc
     1501 gacgagcccg cctgggccgc cgaccgcagc gtgaccgtca cggcgccgca ggtaccgttg
     1561 ccggcgatcg cgctggtctg gctcgcgccg ccggtgacca gcgccgacgc accggcgctg
     1621 cagatcgccg cggcgctgct cggcagcggt gagtcgtcgc ggctgaacca ggcgctcgtg
     1681 taccggcagc gcatcgccgg ccagatcggc ttcgacgccg atctgcgcgc cggcccgggg
     1741 ctgctgaccg ccaccgcgat cgccgccagc ggcaagccgc tcacggcggt cgcgcgggcg
     1801 ctgctggccg aggtgctgcg gctcgcggcg cagccggtcg gcgcggccga gctcgcaaag
     1861 gtcaagacgc agttgttgac ggcagcgctg ctcgcgcgcc agacccccga agggctggcc
     1921 tcggccgtgg ccgacgccgc ggtgctgcag ggcggcgccg ggcacgtgaa caccgacctg
     1981 gtcgcattgc agaacgtcag cgcggccgac gtgcagcgcg tgctgcagcg ctatgtggca
     2041 cgcgcgcaca aggtcagcct gcagtacgtg caggcggcaa cgcaggaagg cggcgcgaag
     2101 tgaacccgtc gcttgcgcga tcgatcggcg cgctgttgct gagcctcgcc gccctggcac
     2161 ccatcgcccg cgcgcagggc ttcgacacgc cgccgcagcc cggcgcgccg cgcccgctga
     2221 ctgttcgcgc gccggtcgag cagcgcctgc cgaacgggct gcgcgtggtg ctggccgagc
     2281 gccgcggcgt gcagctcgtg acggcgcagc tggtcgtgtt gtcgggcagc gaggtcgatc
     2341 cgccgcagcg ggccgggctc gcgtcgatga ccgcggggct gctgaccaag ggcacgcggc
     2401 gccacggcgc gaccgcgctc gcacaggccg ccgagtcgct cggcggcgcg ctcgacagcg
     2461 gcgccggctg gcaccagtcc gaggtcgcga tcacggtcac cgtgccgaag ctcgatgcgg
     2521 cgctggggct ggtcagcgaa gtcgtgcagc agccggtgtt cgcgccggcc gagctcgacc
     2581 ggctgcgcgc ccaggcgctg gacgaactga aggtcgccta cacgcagccg gccacgctcg
     2641 ccggcctggc cgcgcagcgc ctgctgttcg gcagcggcgc ctatggccac ccggccggcg
     2701 gcacgccggc gtcgctggcg cggatcgcgc gcgccgatct gctcgcgctg catgcggccc
     2761 gctaccggcc cgacaacgcg gtgctggtgc tggccggcga tctccacgcc gccgatgcac
     2821 tgcggctcgc gcagcgccac ttcggcgcct ggaaggcccg cggcagcgcg gcggcgcccg
     2881 cgccattgcc tgtgggtgcg gcactggcgc aatccaccgc ggtgatcgac ctgccggcat
     2941 ccggacaggc cgcggtggtc gtcgccgcgg cgctgccgcc gctcggggcc gatcgcgcga
     3001 ccgcggcggt gatgaacgcg gtgctgggcg gcggctactc gtcgcgcctg aaccaggaga
     3061 tccgcatccg gcgcggcctg agctatggcg ccggcagtgc cctcgacgcg tttgccgacg
     3121 gcggctcgct gcgcgcggtg gtccagacca agaacgagtc ggcggccgag gtgctgggcc
     3181 tggtgcagag cgaactcgac cgtcttgcgg cggcacccgt cggcgccgac gaactcgcgg
     3241 cgcgcaaggc gaccttgatc ggcgatttca gccgcagcgt cgagacgacc gcgggcctgg
     3301 gcgcggcggt caaggcgttg atcgtcgccg ggcggccgct ggcggagctg ggtctgcgca
     3361 tcgacgcgct ctcggccgtc ggcgccgacg cggtgcagcg ctacgcggcc gcgcagctcg
     3421 gcacgaacgg ccggcgcgtg gtcgtcgccg gtgacgcggc gcgctttgcg caggccttga
     3481 aggccacgac gccgggcctg tcggtcatcg cgtcagacaa gctcgatctc gatctcggca
     3541 gcgcgctgag cgcgccctga gccggctgcg tcaccgcaag aaacacaatc ccctgaaccg
     3601 ctccggtatt ggattacgcg cggaatccgg aatgctggga caatcgaggg gttgcccgtg
     3661 attggcgtca aagctccacg gtcgtccgca cttcacattt caggaccacc aatggccacc
     3721 cgtcgaccca cctcacccac cgcttccgac accacgcaga agctgcgcca gacccaggcc
     3781 gaatacgtcg ccgcgcgccc ggccggcgag cccggcgtca agccgatgat gtcgagctcg
     3841 aagatgtacg tctgccgccg ctgccaggcc aacttcaaga ccggcgacgg caagcccgac
     3901 gcgcgcctgc cgggcctggg caagtgcaac aagtgcctgg ccgcagccac ggccgcgctc
     3961 gccgcctgag ttcctgagcc ttcaccgcgc ccgctcgggt gcggccagcg cgttcagtgg
     4021 gtcgccgccg cgcggcccat gcgcgcgagg acatgcccgg ccacgcccag cgccagttcg
     4081 tgcgagtcca gcggcgcaca cctggcaggc gcacgctcga tgagggccat cgtggcctgg
     4141 gcccgcgggc gcgtcagcag atcggcatcg gcgactgctt gcggatcgcc tcgtgcgcct
     4201 tggtcatcgc atacgcgagc gcggcgtcgg ccgacggcca gcggtggccg caggcgaggc
     4261 tctcgtcgcg caggatctcg tgcgcggcgc cgaccgtacc ctgctcgcgc agcacgacca
     4321 gcgcggcgat gtagccatca cccatcggcc cttcgagcgc gccggcatag gcgcggtatt
     4381 ccccgatctt gcgttcatcg aaatgcattg aatcgctccg gccggcacac cgagcccgcc
     4441 catgaacgaa tcatccgcgc atatgttgca gtgcggtatc caccgtaggg gggagaaatg
     4501 ccgctgcgcg tggcgaggag gccacagatc gacgcggctc agccgtgcgt gacgcgatgc
     4561 gcctcggcgg cgcgcacgcc gaatccagtt gtgcggcagg gtagctgcct tcgggcccgc
     4621 agccgagcac gtgcccggaa cccgcgaccc gttgtaggac aagcgcgggt tctgcccggc
     4681 gggccttgcc acatgacgga tgccccgcaa aatggtgcaa tgcagcaatt cgacatgacc
     4741 atgagtttcc ggaccctttc cgacacgatc gccagggcgc ttggcttcac cgggctcctt
     4801 tggcgctggg gccgcgtgaa gggcgtgacc ggcgcgcttc gcaccgcgct tgcgaaggtc
     4861 ggcatcgcac cgggcagcgc gccacgcacc acaccgcgcc gcgccgcggc gaccccgacc
     4921 gacgccgtgc ggtttctcag ccgcagcttc acgaacgccg cgggaacccg ggcctacaag
     4981 ctctatgtgc cggcgagcta ccggggaacg gcggtcccgc tgctgatgat gctgcacggc
     5041 tgcaagcaga acccggacga cttcgccgcg ggcacgcgca tgaacgagct ggccgaggcg
     5101 cacggcttcc tcgtcgccta cccggcgcaa tcggagcgcg cgaacggcgc gaactgctgg
     5161 aactggtttc gctccggcga ccaggcccgc gacggcggcg agccctcgat cctcgccggc
     5221 atcgtcgcgg ccatcgccgc cgactacgcg atcgaccggc gccgcgtctt cgtcgccggc
     5281 ctgtcggcgg gcgcggcgat ggccgtgatc ctgggccgca cgcaccccga ggtcttcacc
     5341 gcggtgggcg cgcactcggg cctgccgtac cgcgccgcgc agggcgtggc ctcggcgttc
     5401 gcggcgatgc acggcggcct ggcgcaggcc atcgccggct ggccgccgct gcgcgccgcc
     5461 gatgcggccg cgcgcgcggc agcggtgccc gccgtgccca cgatcgtctt ccacggcgac
     5521 cacgacccga cggtggcggc gtcgaacagc gacgccatcg tcgcgcaggc cctggcggca
     5581 ccgcaggctt cggcgctgct cgagagccgg cgtctcgagc ccgcctcggc ggtttcgcgc
     5641 ggccacacga acacggccta cacgacgccg gacgggcgca cgctggtcga gcaatggacc
     5701 ctgcacggcg gcgcgcacgc ctggtccggc ggcagcccgg agggctcgta cacggaggca
     5761 caggggcctg atgcatcggc cgagatggtc cgcttcttcc tcgcgcagta gcgcccgggg
     5821 gatcagcccg ccgcgcccat tcgccaaggc gccgcgatcg caaacggggg cgatgcacga
     5881 tcggttgctg tggtccagac caccgccgcc ctcgcgcgcg acccacacga tcttccgcgt
     5941 cgggtcctcg atgccatgcg ggttttccgc caggcgaaca aacgaacaaa ccagtttgtt
     6001 ttcacttaac atcgccgtca catcacaacc ctgcccggaa attgccatga ttccacgcga
     6061 cattctggtc gttgccgctg cacgcaccgc gatcggctca ttcggtggct cgctgaagga
     6121 cgtgcccttg cgccaactcg ccaccacggc cgtgcgcggc gcgctgcaac gcagtggcgc
     6181 ggcagcggca caggtgggcc aggtggtgat gggcaacgtc atccccaccg aaccggcgga
     6241 cgcctatctc agccgcgtgg ccgccgtcga t
//
LOCUS       DATMJB010000490         4910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_376088, whole genome shotgun sequence.
ACCESSION   DATMJB010000490 DATMJB010000000
VERSION     DATMJB010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376088"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VNU48_10500"
     CDS             <1..644
                     /locus_tag="VNU48_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
                     /protein_id="HWI11749.1"
                     /translation="GLAALMSHLGLGAEFGNIIMLALLGFVAFIAIRLLMNRFGRSAA
                     PAAMATPGGLQFAGAPPPTGNASSALAPPAAPLAQAAAGAASSLPAGFDAAAFERIAK
                     MIFIRMQTANDSADLNDLRAFTTPEMFAAVKLELQERAGAAQATDVVRIDAEVLDVTS
                     EPQRQLVSVRFHGLIREEHDGAAAPFDEVWHLVKPADGSREWAIAGIQQSQAA"
     gene            complement(758..1501)
                     /locus_tag="VNU48_10505"
     CDS             complement(758..1501)
                     /locus_tag="VNU48_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe dioxygenase family protein"
                     /protein_id="HWI11750.1"
                     /translation="MTILLTPLLTAPAQLDAALRGRGHAVLGAAGLRELVHATADELD
                     ALRPAWSDLPPDGYLRDGGRYRRRRHACFVVEGDAVQAVPHRAHWQPIEYNALHGGLE
                     RWFEPIRDEVIARPVWARLLVALSAHCSALKGAQRWYVEAHQFRIDTTDGIGRPTPEG
                     AHRDGVDLVAVLLIDRTGIKGGETRVFEADGPAGLRFTMTEPWTTLLLDDARVIHEST
                     PIQPLADAGHRDTLVLTWRAGGFQGPPGR"
     gene            complement(1626..1856)
                     /locus_tag="VNU48_10510"
     CDS             complement(1626..1856)
                     /locus_tag="VNU48_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HWI11751.1"
                     /translation="MQLIDFELRGDHITLDALLKATGLAPSGGIAKMMVADGRVQVDG
                     IAESRKTCKIRAGQVVSLAGVRVRVLAAVSNS"
     gene            complement(1847..2845)
                     /locus_tag="VNU48_10515"
     CDS             complement(1847..2845)
                     /locus_tag="VNU48_10515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWI11752.1"
                     /translation="MRLSVLDQSVSVAGSTEGAAIRDTLALAQDCERLGYARFWVSEH
                     HGLPTIVGSAPEMLMAAIAARTTRIRIGSAGVMLPHYSALKVAEQFRVLEALAPGRID
                     LGVGRAPGGDMRTARALNPNAHAAADAFPEQVRDLQAWTALPSHLGIAAHPLGEHAPE
                     VWILGSSNYGAQLAAHFGLPYAYAYFFVDGHGADEALALYRRLYRPSERHPEPQATVC
                     VWALVADTADEATHHALGRERWRLDRERGILGPLQAPDATAARGFTDAEWPTVEAMRR
                     KAFVGDAAQVGAKLRALAIELQLDEIVVNTWAHEPQVRRDSYALLAAEFGLEAACS"
     gene            complement(2855..3313)
                     /locus_tag="VNU48_10520"
     CDS             complement(2855..3313)
                     /locus_tag="VNU48_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWI11753.1"
                     /translation="MTQHWKHDGVRVIGADSLDPNTAQTPGMDRKAAISFARVGANKL
                     WAGTVHIHPDAKTGAHHHGPLESVIYVVKGRARMRWGDALEFTAEAGPGDFIYVPPFV
                     PHQEINASATEVLECVLVRSDGEAVAVNLDIAPVEKPEQVRWIDPTHPQG"
     gene            3583..3792
                     /locus_tag="VNU48_10525"
     CDS             3583..3792
                     /locus_tag="VNU48_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWI11754.1"
                     /translation="MTTQTGTVKWFNESKGFGFIAQDAGGADLFAHFRDIQGNGFKTL
                     LENQRVEFEVKQGQKGLQAANIKVL"
     gene            3931..>4910
                     /locus_tag="VNU48_10530"
     CDS             3931..>4910
                     /locus_tag="VNU48_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWI11755.1"
                     /translation="MTAALAALTNHQFRLKARPNGLPTREGWDFTTEPVAEPADGGVL
                     VKTLAISLDPAMRGWMNEGRSYIAPVAIGEVMRAGGIGEVIASKNPGFKVGDVVSCKP
                     GVQEYCLIAADQIEANGLFRIDTRLGTPNQWLNVLGMPGMTGYFGLMDVGQPKAGETV
                     VVSGAAGAVGQTVGQLAKIRGCRVVGIAGGAAKCAWVVKELGFDACIDYKAGNVKADL
                     KDACPKGVDIYFDNVGGEILDTVLTRLARGARIIICGAISQYNNTTPVKGPANYLSLL
                     VNRASMTGMVVFDYADRYGEAAREMAGWIAQGKLKAKEDVVAGGIDAFPET"
BASE COUNT          743 a         1724 c         1709 g          734 t
ORIGIN      
        1 tgggcctcgc cgcgttgatg agccacctgg gcctgggcgc cgagttcggc aacatcatca
       61 tgctggcgct gctgggcttc gtcgcgttca tcgcgatccg cctgctgatg aaccgcttcg
      121 gccgcagcgc cgcgccggcc gcgatggcga cgccgggcgg tctgcagttc gccggcgcgc
      181 cgccgcccac cggcaacgcg tcgtcggccc tggcgccacc ggccgcaccg ttggcgcagg
      241 ccgcggcagg cgcagcgtcg tcgctgccgg cgggcttcga tgcggccgcg ttcgaacgca
      301 tcgcgaagat gatcttcatc cgcatgcaga ccgccaacga cagcgccgat ctgaacgacc
      361 tgcgcgcctt cacgacgccc gagatgttcg ccgccgtcaa gctcgagctg caggagcgcg
      421 ccggtgccgc acaggcgacc gatgtcgtgc gcatcgacgc cgaggtgctc gacgtgacga
      481 gcgagccgca gcgccagctc gtcagcgtgc gcttccacgg cctgatccgc gaagagcacg
      541 atggcgcggc cgcgccgttc gacgaggtct ggcacctggt gaagccggcc gacggcagcc
      601 gcgagtgggc gatcgccggc atccagcaat cgcaagcggc ctgaaggaga acggcagcgt
      661 tcggcgacgc cgtgccgtcg ccgcgcggcg cagccggacg aacccgcagt ggatgggcag
      721 gccgcatgtc ggatcgcacg acacgggcgc cgacgcttca gcggcccggc ggcccctgga
      781 agccgccggc acgccaggtc agcaccagcg tgtcgcggtg gcccgcatcg gcgagcggct
      841 ggatcggcgt gctctcgtgg atcacgcggg cgtcgtcgag cagcagcgtg gtccagggct
      901 cggtcatcgt gaagcgcagc ccggccgggc cgtcggcctc gaacacgcgc gtctcgccgc
      961 ccttgatgcc ggtgcggtcg atcagcagca ccgccaccag gtcgacgccg tcgcggtgcg
     1021 cgccttccgg cgttggccgt ccgatgccgt cggtcgtgtc gatgcggaac tggtgcgcct
     1081 ccacgtacca gcgctgcgcg cccttcagcg cgctgcaatg cgcgctcagc gccacgagca
     1141 gccgcgccca caccggccgc gcgatcacct cgtcgcggat cggctcgaac cagcgttcca
     1201 ggccgccgtg cagcgcgttg tattcgatcg gctgccagtg cgcgcggtgc ggcaccgcct
     1261 gtaccgcgtc gccctcgacg acgaagcatg cgtggcgccg acgccggtag cggccgccgt
     1321 cgcgcaggta gccgtcgggc ggcagatcgc tccacgccgg gcgcagtgca tcgagctcat
     1381 cggcagtcgc atgcaccagc tcgcgcaggc cggcggcgcc gagcaccgcg tggccgcgcc
     1441 cgcgcagcgc ggcgtcgagt tgcgcgggcg cggtcagcaa gggggtcagc aagatcgtca
     1501 tggccgccat gatgccgtga cttgcgcccg gcgcgcttca cactgtcaag cagtcgccgt
     1561 gcttcaatcc cggtagcgcc cgaccgaccg aagacctgtt ctcggtcggc gtcaactacc
     1621 gcgtcttaag agttcgacac ggcggcgagc acccgcaccc gtacaccggc caggctgacg
     1681 acctggcccg cgcggatctt gcaggtcttg cgcgactccg cgatgccgtc gacctgcacg
     1741 cgcccgtcgg ccaccatcat cttcgcgatg ccgccgctgg gcgccaggcc cgtggccttc
     1801 agcagcgcat cgagcgtgat gtggtcgccg cgcaattcga agtcgatcag ctgcatgccg
     1861 cttcgagccc gaactcggcc gcgagcagcg cgtacgaatc gcgccgcacc tgcggctcgt
     1921 gagcccaggt gttgacgacg atctcgtcga gctgcagttc gatcgcgagc gcgcgcagct
     1981 tcgcaccgac ctgcgcggca tcgccgacaa aggccttgcg gcgcatcgcc tcgacggtcg
     2041 gccattcggc gtcggtaaag ccgcgcgccg cggtcgcgtc gggcgcctgc agcgggccca
     2101 agatgccgcg ctcgcggtcg agccgccagc gttcgcggcc gagcgcgtga tgggtcgcct
     2161 cgtctgccgt gtccgccacc agcgcccaca cgcacaccgt ggcctgcggc tcggggtggc
     2221 gctcgctcgg ccggtacagg cgccggtaca gcgccagcgc ctcgtcggcg ccatggccgt
     2281 cgacgaagaa gtacgcgtag gcgtagggca ggccgaagtg cgccgcgagt tgtgcgccgt
     2341 agttcgagct gccgagaatc cagacctcgg gcgcgtgctc gcccagcggg tgcgcggcga
     2401 tgcccagatg cgacggcagc gcggtccagg cctgcaggtc gcgcacctgc tcggggaacg
     2461 catcggcggc cgcatgcgca ttcgggttca gcgcacgtgc ggtgcgcatg tcgccgccgg
     2521 gcgcgcggcc gacgccgagg tcgatacgcc ccggcgcgag cgcctccagc acgcgaaact
     2581 gctcggccac cttcagcgcg ctgtagtgcg ggagcatcac gccggcgctg ccaatgcgga
     2641 tgcgcgtggt gcgcgcggcg atcgcggcca tcagcatctc gggcgcgctg ccgacgatgg
     2701 tgggcaggcc gtggtgctcg ctgacccaga agcgcgcgta gcccaggcgc tcgcagtcct
     2761 gcgccagggc cagcgtgtcg cggatcgccg cgccttcggt gctgccggcc accgagacgg
     2821 attggtcgag aacggagagt cgcaagccca tcgctcagcc ttgcggatgc gtcggatcga
     2881 tccagcgcac ctgctccggc ttctcgaccg gcgcgatgtc caggttcacc gcgaccgcct
     2941 cgccgtcgct gcgcaccagc acgcattcga gcacctcggt cgcgctggcg ttgatctcct
     3001 ggtgcggcac gaacggcggc acataaatga agtcgcccgg cccggcctcg gcggtgaact
     3061 cgagcgcgtc gccccagcgc atccgggccc ggcccttgac gacatagatc acgctctcga
     3121 gcgggccgtg atgatgcgca ccggtcttcg cgtcggggtg gatgtgcacg gtgccggccc
     3181 acagcttgtt cgcaccgacg cgcgcgaaac tgatcgcggc cttgcggtcc atgcccggcg
     3241 tctgcgcggt gttcggatcg agcgagtcgg cgccgatcac gcgcacgccg tcgtgcttcc
     3301 agtgctgcgt catgcgcgtt cctgtggtcg tttgtgtcga ttctgaacga cagtcgcagc
     3361 ctgtgcgcag cagggagatc ggcgcggcgc acggtcggcg ggcttttcgt gtacattcgc
     3421 cccacagtta tgcgcaaggc agtggttgtt cgagattctt ccccccacct gtcgcggtcc
     3481 tcgcggcggg cgtcgttaga aatccccccg attcgcactt gagcgtcgct gcatgcgctg
     3541 catgtgctgc gcattatttg aaaatagata ggatagtttt ccatgacgac ccaaaccggt
     3601 accgtgaagt ggttcaacga gagcaagggc ttcggcttca tcgctcaaga cgcgggcggc
     3661 gccgacctgt tcgcccactt ccgtgacatc cagggcaatg gcttcaagac cctgctggaa
     3721 aaccagcgcg tcgagttcga agtcaagcaa ggccagaagg gcctgcaggc agccaacatc
     3781 aaggtgctgt aaagcccggg cgcaagcccg gccacaagga accgcggcga tgccgcggtt
     3841 tttttatgcc cgcgcgcagc gtcgcgtcgg tgacgcggcg ccgtcgcggc gcggcctaag
     3901 atgcgcgcct ggcctttcag gagacccgcc atgaccgccg ccctcgccgc gctgaccaac
     3961 caccagttcc gcctgaaggc gcgcccgaac ggcctgccca cgcgcgaggg ctgggacttc
     4021 acgaccgagc cggtcgccga accggccgac ggcggcgtgc tcgtcaagac gctcgcgatc
     4081 tcgctcgacc cggcgatgcg cggctggatg aacgagggca ggagctacat cgcgccggtg
     4141 gcgatcggcg aggtcatgcg cgccggcggc atcggcgagg tgatcgcgtc gaagaacccg
     4201 ggcttcaagg tcggcgacgt ggtcagctgc aagcccggcg tgcaggaata ctgcctgatc
     4261 gcggcggacc agatcgaggc caacggcctg ttcaggatcg acacgcgcct gggcacgccg
     4321 aaccaatggc tcaacgtgct gggcatgccc ggcatgaccg gttacttcgg cctgatggac
     4381 gtgggccagc cgaaggccgg cgagactgtc gtcgtctccg gcgcggccgg tgcggtcggc
     4441 cagaccgtcg gccagctcgc gaagatcagg ggctgccgcg tcgtcggcat cgccggcggc
     4501 gcagccaagt gcgcgtgggt ggtcaaggag ctcggcttcg acgcctgcat cgactacaag
     4561 gccggcaacg tgaaggccga cctgaaggac gcctgcccga agggcgtgga catctacttc
     4621 gacaatgtcg gcggcgagat cctcgacacc gtgctgacgc gcctggcgcg cggcgcccgc
     4681 atcatcatct gcggcgcgat cagccagtac aacaacacca cgccggtcaa gggcccggcc
     4741 aactacctgt cgctgctggt caaccgggcc agcatgaccg gcatggtggt gttcgactac
     4801 gccgatcgct atggtgaggc cgcgcgcgag atggccggct ggatcgcaca gggcaagctc
     4861 aaggcgaagg aagacgttgt cgccggcggc atcgatgcat tcccggagac
//
LOCUS       DATMJB010000491         5743 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_197343, whole genome shotgun sequence.
ACCESSION   DATMJB010000491 DATMJB010000000
VERSION     DATMJB010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5743
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197343"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VNU48_10535"
     CDS             complement(<1..342)
                     /locus_tag="VNU48_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI11756.1"
                     /translation="MTEGSTWQAEKSLLMQTQIAQAAMQCLIEIGYAQTTTDKIAQKA
                     GVSRGAMTHHFKSRAAVFIAAATYIFELRAIEYGEAIGKVNVPAGTLPTYEDMRNTVV
                     SLQKYYARPSFI"
     gene            complement(448..1227)
                     /locus_tag="VNU48_10540"
     CDS             complement(448..1227)
                     /locus_tag="VNU48_10540"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI11757.1"
                     /translation="MNAAPSTLFSLTGRVALVTGASSGFGAHFAAVLARAGAKVACVA
                     RRRDRVEALAGALRDTGAQAVACAMDVTDRDSIRAGFDTVEAALGTVDVLVNNAGLSA
                     PAPFPQMSDEQWTSVLDVNLTAPYRVSREMCQRLIAKGQPGSIIHIASILGHLAKAMF
                     INYGTTKGALINLTQAMALDLLPHGIRVNAIAPGYFPTEMTNPFFETDAGKKEIAALP
                     PGRLGRLDELDGPLLLLASDASSYMSGSVLTVDCGHSVRLS"
     gene            complement(1224..2000)
                     /locus_tag="VNU48_10545"
     CDS             complement(1224..2000)
                     /locus_tag="VNU48_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWI11758.1"
                     /translation="MMVSDRSSAWPANLPPMPMVDFARFGAVEPVPLTRHQKLAASFL
                     ARNWAQIPHVTHHDEADIDAAEALRQRLAQEQGGKLSLVPLLIKAAVNALRALPRFNA
                     SLVGDTLVLKRYFHIGVAVETPAGLVVPVIRDCERKSIAELAAELTDKAGRARTKGLP
                     MSEMSGGCFTISSLGGIGGTAFTPIINAPEVAILGVTRSAERLTLVDGRPCSRRVLPL
                     SLSYDHRVINGADAARFCRAVAEALARPDGLLHDPSSSLP"
     gene            complement(1997..2221)
                     /locus_tag="VNU48_10550"
     CDS             complement(1997..2221)
                     /locus_tag="VNU48_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HWI11759.1"
                     /translation="MQILLPKLGFSVNEANLVEWMVADGATVAKGDPLYALESEKSVQ
                     DIDAPIAGVVKILKPAGEVYEVGTVIGEIA"
     gene            complement(2249..3241)
                     /locus_tag="VNU48_10555"
     CDS             complement(2249..3241)
                     /locus_tag="VNU48_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWI11760.1"
                     /translation="MSSTAKSMNMIQAINLALDHAMEQDPTVLVLGEDIADRQGGGVF
                     KCTAGLSAKYGIERVKSTPIAEQAIMGAAVGAALGGMRPVAEIMLMNFMTVGMDQFVN
                     HAAKLRFMTGGQTHVPLTVRTATGAGFGTGGQHADYLESWFAHTAGIKVVAPSTPADA
                     YGLMLSCIFDDDPCLFIENLPLYFVAGAAPVPGTRVPIGKAKVVREGRDVTVIGYSRT
                     MLEVLAVADDLAKRDISVEVIDLRSISPLDMDTLRASVAKTRRAVVAHEAQVDFGVGA
                     EIAARLHRDLFGQLLAPVGRVGAARSPVPFSKALESAYLPTPERISAAILQALA"
     gene            complement(3238..4230)
                     /locus_tag="VNU48_10560"
     CDS             complement(3238..4230)
                     /locus_tag="VNU48_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWI11761.1"
                     /translation="MTISETTSPSRRTLLEIYRIATLIKQADDRFIELIKAGQIASPY
                     YSPRGQELIPAAVSVNLRPSDVVVTTYRGIHDQLAKGIPLEPFVAEFMGKATGTCKGK
                     GGPMHITHVASGLPVTTGVVGSGLPIANGLAWAAQLKKTGQVTVCNFGDGATNIGAFH
                     EALNLASLWKLPVVFVCQNNGYAEHTKFELGTSSRTIAERAVSYGMRGVEVDGNDAVA
                     MWAAAKLAIDRARAGDGPTLLDAKTFRFRGHLLGDDSHYIAKDEMAQAVAADPLPRLR
                     AWLIAQGHASDAALTDIERSNEEAFERAVQFGLGSPFPDPSELERDVVAAEIAA"
     gene            complement(4440..4823)
                     /locus_tag="VNU48_10565"
     CDS             complement(4440..4823)
                     /locus_tag="VNU48_10565"
                     /inference="COORDINATES: protein motif:HMM:NF040046.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HWI11762.1"
                     /translation="MRDNAHGERLLETARDVLRGELLPALPAQQRHAALMVANAMAIA
                     ARELNRHDAAAPNDARAAEDRRLCASIRAGRVDAGAAAAQLHAELLASTRARVEVNNP
                     KYLVPSPHSLTRDHVAEPPCPDRRT"
     gene            complement(4823..>5743)
                     /locus_tag="VNU48_10570"
     CDS             complement(4823..>5743)
                     /locus_tag="VNU48_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HWI11763.1"
                     /translation="GGAIQQNWRLRARVEGGAHAGEAQWVLRTDAPSAVSASHGRAQE
                     FALLSAAAQAGVTVPQPLWVCEDRAIIGRPFFVMRCVAGTAAGHVLTRDSSPVTDRVQ
                     LTQRLGHELALIHSIRPPRADLDFLPACDEAPARQAIRRLRRDLDGLPTAHPALEWGL
                     RWLERQAPPQHEIVLCHGDYRTGNYLVDAQGLTGILDWEFAGWGDALQDIGWFCARCW
                     RFGADTREAGGIGQRADFYRGYERASGRPLDRGQVRYWEVMAHVRWAVIALAQAQRHA
                     SGEETSLLLALTGRIVPELEHEVLTMTEPD"
BASE COUNT          831 a         1928 c         2042 g          942 t
ORIGIN      
        1 gatgaacgac ggccgggcgt aatacttctg cagcgagacg acggtgttgc gcatgtcctc
       61 gtacgttggc aaggtgccgg ccggcacgtt gaccttgccg atcgcctccc catactcgat
      121 ggcacgcagc tcgaagatgt aggttgccgc cgcgatgaac accgcggcgc gcgatttgaa
      181 gtgatgcgtc atcgcgccgc gcgacacgcc ggccttctgc gcaatcttgt cggtcgtggt
      241 ctgggcgtag ccgatctcga tcaggcactg catcgcggcc tgcgcgatct gcgtctgcat
      301 cagcaggctc ttctcggcct gccaggtgct gccctcggtc atgttgcggg ggcgggtcgg
      361 gcggggtttc ttggcggtcg gcatggtcat ctggggcggg gggcatggtg acgatccccg
      421 tattttgtgt gcacggcgaa ccgggtgtca cgacagtcgg accgagtgac cacagtccac
      481 ggtgagtacc gagccgctca tgtacgacga tgcgtcgctg gccagcagca gcagcggccc
      541 gtcgagttcg tcgagccggc ccaggcggcc cggcggaagg gccgcgatct ccttcttgcc
      601 cgcgtcggtc tcgaagaaag ggttggtcat ctcggtcggg aaatagcccg gtgcgatcgc
      661 attcacgcgg atgccgtgcg gcaacaggtc cagcgccatg gcctgggtca ggttgatcag
      721 cgcgcccttg gtggtgccgt agttgatgaa catcgctttg gccagatggc ccaggatcga
      781 ggcaatgtgg atgatcgacc cgggctgccc tttggcgatc agtcgctggc acatctcgcg
      841 cgagacgcgg tacggtgccg tcaggttgac gtcgagtacc gaggtccact gttcgtcgct
      901 catctgcgga aacggtgccg gcgccgacag gccggcattg ttgaccagca cgtccacggt
      961 gccgagcgcg gcctcgaccg tgtcgaagcc ggcccggatg gagtcccggt cggtcacgtc
     1021 catcgcgcag gccacggcct gcgcgccggt gtcgcgcaac gcgccggcga gcgcctcgac
     1081 gcggtcccgg cgccgtgcca cgcaggccac cttcgcaccg gcgcgggcca gcactgccgc
     1141 gaagtgcgcg ccgaagccgg aactcgcgcc ggtcaccagg gccacgcggc cggtgagcga
     1201 gaacagggtg gacggggcag cgttcatggc aggctcgatg atgggtcgtg gaggaggccg
     1261 tcgggccgcg ccagcgcctc cgccacggcg cggcagaaac gcgccgcatc ggctccgttg
     1321 atgacgcggt ggtcgtagct cagcgacagc ggcagcacgc ggcggctgca gggccggccg
     1381 tcgaccaggg tcagccgctc ggcgctgcgc gtcacgccga ggatggcgac ctcgggcgcg
     1441 ttgatgatcg gcgtgaaggc ggtgccgccg atgccgccga gcgagctgat cgtgaagcag
     1501 ccgccggaca tttcgctcat cggcaggccc ttggtgcgtg cgcgtccggc cttgtcggtg
     1561 agttcggcgg ccaactcggc aatcgacttg cgctcgcagt cgcggatcac cggcacgacc
     1621 agtccggccg gcgtttccac cgcgacgccg atgtgaaagt agcgcttgag caccagcgtg
     1681 tcgccgacca gcgaagcgtt gaagcggggc agggcgcgca gggcattcac cgcggccttg
     1741 atcaacagcg gcaccaggct gagcttgccg ccctgttctt gtgccaggcg ttggcgcagg
     1801 gcctcggcgg cgtcgatgtc ggcctcgtcg tgatgggtga cgtgggggat ctgcgcccag
     1861 ttgcgggcca ggaacgacgc cgccagcttc tggtggcgcg tcagcggcac cggttccacc
     1921 gcgccgaacc gggcgaagtc gaccatcggc atcggcggca ggtttgccgg ccaggcgctg
     1981 gacctatccg aaaccatcat gcgatctcgc cgatcaccgt gcccacttca tagacctcgc
     2041 ccgccggctt gaggatcttc accacgccgg cgatcggtgc gtcgatgtcc tgcaccgact
     2101 tctcgctctc cagcgcgtac agcgggtcgc ccttggcgac ggtggcgccg tcggccacca
     2161 tccattcgac gagattggcc tcgttgaccg agaagccgag tttgggcagc aggatctgca
     2221 tggttggcaa ttcgttgagt cagttgaatc aggcaagcgc ttgaaggatg gcggcgctga
     2281 tgcgctccgg cgtgggcagg taggccgatt cgagcgcctt cgagaagggc accggcgaac
     2341 gcgccgcacc gacgcgcccg accggcgcca gcaactggcc gaacaagtcg cggtgcagcc
     2401 gcgcggcgat ttccgcgccg acgccgaagt cgacctgggc ctcgtgcgcc accacggcac
     2461 gccgcgtctt ggccaccgag gcgcgcagcg tgtccatgtc cagcggcgag atgctgcgca
     2521 ggtcgatgac ctcgaccgag atgtcgcgct tggccaggtc gtcggccaca gccagcactt
     2581 ccagcatcgt gcggctgtag ccgatcacgg tgacatcgcg gccttcgcgc acgaccttgg
     2641 ccttgccgat cggcacgcgc gtacccggca ccggcgccgc gccggccacg aagtacagcg
     2701 gcaggttctc gatgaacagg caggggtcgt cgtcgaagat gcacgacagc atcaggccgt
     2761 aggcatcggc cggggtggac ggtgccacca ccttgatgcc ggcggtgtgc gcgaaccagc
     2821 tctccaggta gtcggcgtgc tggccgccgg tgccgaagcc cgcgccggtg gcagtgcgca
     2881 cggtcagcgg cacgtgggtc tggccgccgg tcatgaagcg cagcttggcg gcgtggttta
     2941 cgaactggtc catgcccacg gtcatgaagt tcatcagcat gatctcggcc accgggcgca
     3001 tgccgccgag tgccgcgccg acggccgcac ccatgatggc ctgctcggcg atcggcgtgg
     3061 acttgacgcg ttcgatgccg tacttggccg acagcccggc ggtgcacttg aagacgccgc
     3121 cgccctgcct gtcggcgatg tcctcgccga gcaccagcac cgtcgggtcc tgttccatcg
     3181 cgtggtccag cgccaggttg atggcctgga tcatgttcat cgacttcgcg gtgctgctca
     3241 tgctgcgatc tccgcggcaa cgacgtcccg ctcgagttcc gaggggtcgg ggaaggggct
     3301 gcccagaccg aactgaacgg cgcgctcgaa ggcttcttcg ttgctgcgct cgatgtcggt
     3361 cagggcggcg tcgctggcgt ggccctgggc gatcagccag gcgcgcaggc gcggcagcgg
     3421 atcggcggcc acggcctgcg ccatctcgtc cttggcgatg tagtgactgt cgtcgcccag
     3481 caggtggccg cggaagcgga aggtcttggc gtcgagcagg gtcggcccgt cgcccgcgcg
     3541 cgcacggtcg atggccagct tggcggccgc ccacatcgcc accgcatcgt tgccgtcgac
     3601 ctcgacgccg cgcatgccgt aggacacggc gcgctcggcg atcgtgcgcg acgaggtgcc
     3661 gagctcgaac ttggtgtgct cggcgtagcc gttgttctgg cacacgaaca ccaccggcag
     3721 cttccacagc gacgcgaggt tcagcgcctc gtgaaaggca ccgatgttgg tggcgccgtc
     3781 gccgaagttg cacaccgtga cctggccggt cttcttcaac tgcgccgccc aggccagccc
     3841 gttggcgatc ggcagccccg agccgaccac gccggtggtg accggcagcc ccgaggccac
     3901 atgcgtgatg tgcatcggcc cgcccttgcc cttgcaggtg ccggtggcct tgcccatgaa
     3961 ctcggcgacg aagggctcga gcgggatgcc cttggcaagc tggtcgtgga tgccgcgata
     4021 ggtggtcacg accacgtcgc tgggccgcag gttcaccgag accgccgccg ggatcagctc
     4081 ctggccgcgc ggcgagtagt agggcgaggc gatctgcccg gccttgatca gctcgatgaa
     4141 gcggtcgtcg gcctgcttga tcagcgtggc gatgcggtaa atctccagca gggtccggcg
     4201 actcggggag gttgtctcgc tgatggtcat gggtcacctc gtgtcggatc gggttttgat
     4261 tacagacaag cacgtttgtt tttttgacta tagcaaggcg aagcgaatcg gtgcccaggg
     4321 tttgtccgta agcgcgggga ctttcgcagc ggctgtggcg cgattgccgg agccgcggct
     4381 cgcttgcccc gcgcgcaacg ccgtatcagc gcccgtggaa gatgcgcgcg atcgcgtctt
     4441 cacgttcgac gatcagggca ggggggctct gcgacatggt ctcgggtgag cgaatgcggt
     4501 gatggcacga ggtacttcgg gttgttcacc tcgaccctgg cgcgcgtgct cgccaacagt
     4561 tcggcatgca gctgcgccgc cgcggcgccg gcatcgaccc ggcccgccct gatcgaggcg
     4621 cacagccggc gatcctccgc cgcgcgtgcg tcgttcggcg cggcggcgtc gtgccggttc
     4681 agttcgcgcg ccgcgatggc catcgcgttg gcgaccatca gcgccgcgtg gcgctgctgc
     4741 gccggcagtg ccggcagcag ttcgccgcgc agcacgtcgc gcgcggtctc gagcagccgc
     4801 tcgccgtggg cgttgtcgcg catcagtcag gctccgtcat ggtcagcact tcgtgctcga
     4861 gctccggcac gatgcgcccg gtcaaggcca gcagcagcga ggtctcttcg ccgctggcgt
     4921 gccgttgcgc ctgcgccagc gcaatgactg cccagcgcac atgggccatc acctcccagt
     4981 agcgcacctg cccgcggtcc agcggccgac cgctggcgcg ctcgtagcca cgatagaagt
     5041 ccgcgcgctg gccgatgccg ccggcctcgc gcgtgtccgc gccgaagcgc cagcagcgcg
     5101 cgcagaacca gccgatgtcc tgcagcgcgt cgccccagcc ggcgaattcc cagtcgagga
     5161 tgccggtcag gccctgtgcg tcgacgaggt agttgccggt gcggtagtca ccgtggcaaa
     5221 gcacgatctc gtgttgcggc ggggcttgcc gctcgagcca gcgcaggccc cattcgagcg
     5281 ccggatgcgc cgtcggcagg ccatcgaggt cgcggcgcag gcggcggatc gcctgccgtg
     5341 ccggcgcctc gtcgcaggcc ggcaggaagt ccagatcggc gcgcggcggg cggatcgaat
     5401 ggatcagcgc cagctcgtgc ccgagtcgct gcgtgagctg cacccggtcg gtgaccggac
     5461 tgctgtcgcg tgtcagcacg tggccggccg ccgtgccggc gacgcagcgc atcacgaaga
     5521 acgggcggcc gatgatggcg cggtcttcgc acacccacaa cggttgcggt acggtgacac
     5581 cggcctgggc cgcggcactc agcagggcga attcctgcgc ccggccgtgg ctggccgaca
     5641 cggccgaggg cgcatcggtg cgcagcaccc actgcgcctc gccggcatgc gcgccgccct
     5701 cgacgcgcgc gcgcagccgc cagttctgct ggatcgcgcc gcc
//
LOCUS       DATMJB010000492         2320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_730650, whole genome shotgun sequence.
ACCESSION   DATMJB010000492 DATMJB010000000
VERSION     DATMJB010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730650"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VNU48_10575"
     CDS             <1..1164
                     /locus_tag="VNU48_10575"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HWI11764.1"
                     /translation="RDTLAPRAVGLRVFAVATPDGYRVMPGGLTRVAVDGDSRVIAMQ
                     RGGRSKDTWVLSGAPVNASFSLLSTTVKPEDLVGARVNLPSRAAENLFWYGRYGERCD
                     GALRTLRVAIAGVLDHATEPVDGLAPAIVLAHRLGLIDDMADANVALLRAATHPDEAL
                     ANRLRQLQRTAFNLRDRMSADNWRTLNQLAADPIFQRGAGLPLALSWLDRAVTSMMTL
                     SGYVLDGMTRSVGWRFLSIGRRIERLSHLCVSLQVATTDGAAHGLDWLLDLTDSTGTF
                     RSRYLVAPEWLPVLDMLLRDATNPRSVAFQVKGLAEYIAKLELSHGRFAGDVLAPAQA
                     ALRELAPADLHPDSEAVAQLLEQLRKCAGAVSDELTLKFFSHAASRSVLSLVA"
     gene            1175..2149
                     /locus_tag="VNU48_10580"
     CDS             1175..2149
                     /locus_tag="VNU48_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWI11765.1"
                     /translation="MSEPQRYTVQHETRYAYTAPVSQSWQLARLTPRLLPWQRLLAYS
                     LEIEPRADERRDEVDSFGNMVTHFGLHGAHRMLRVRMECVVEVTERPAIDTAAAPIAQ
                     QQTQLQGEAAEPTQTQTQTQAVAAAPLAWEAVREAIRREPQQDELVPASMSEPTPLVP
                     LSEGARQYAAASFARGRPWLEALTELMGRIHDDFEFEPGATTVATSVDEVLYQKRGVC
                     QDFAHLMLACLRGHGLPARYVSGYLLTDPPPGMARLMGADASHAWVAAYSPQHGWVEF
                     DPTNNQLADQRYITLTWGADFADVVPLRGVILGGGEQRMEVEVSVIPA"
BASE COUNT          325 a          813 c          848 g          334 t
ORIGIN      
        1 cgcgacacgc tcgcgccgcg ggccgtgggc ttgcgcgtgt tcgcggtcgc gacgccggat
       61 ggctaccgcg tgatgccggg cggcctgacg cgcgtcgcgg tcgacggcga ctcgcgcgtg
      121 atcgcgatgc agcgcggcgg gcgttcgaag gacacctggg tgctgtcggg cgcgccagtc
      181 aacgcgtcgt tctcgctgct gtctacgacc gtcaagcccg aggacctggt cggcgcgcgc
      241 gtcaacctgc cctcgcgcgc ggccgagaac ctgttctggt acggccgcta cggcgagcgt
      301 tgcgacggcg cgctgcgcac gctgcgggtg gcgatcgccg gcgtgctcga ccacgcgacc
      361 gagccggtcg acggcctggc gccggcgatc gtgctggcgc accggctcgg cctgatcgac
      421 gacatggccg acgcgaacgt cgcgctgctg cgcgccgcga cgcatccgga cgaggcgctg
      481 gcgaaccggc tgcgccagct gcagcgcacg gcgttcaacc tgcgcgaccg catgtccgcc
      541 gacaactggc gcacgctgaa ccagctcgcc gccgatccga tcttccagcg cggtgccggc
      601 ctgccgctgg cattgagctg gctggatcgc gcggtcacct cgatgatgac cctgtcgggc
      661 tacgtgctcg acggcatgac gcgcagcgtc ggctggcgct tcctgtcgat cgggcggcgc
      721 atcgaacggc tgtcgcacct gtgcgtgagc ctgcaggtcg cgaccaccga cggcgccgcg
      781 catgggctgg actggctgct cgacctgacc gattcgaccg gcaccttccg ctcgcgctac
      841 ctggtcgcgc ccgagtggct gccggtgctc gacatgctgc tgcgcgacgc caccaacccg
      901 cgctcggtcg cgttccaggt caagggcctc gccgagtaca tcgcgaagct cgagctgagc
      961 cacggtcgct tcgccggcga cgtgctcgcg ccggcgcagg ccgcgctgcg cgagcttgcg
     1021 ccggccgatc tgcatccgga cagcgaagcg gtcgcgcaac tgctcgagca gctgcgcaag
     1081 tgcgcgggtg ccgtctccga cgagctgacg ctgaagttct tctcgcatgc cgcgtcgcgc
     1141 agcgtgctgt cgctggtggc ctgagccagg tgcgatgagc gaaccacaga ggtacacggt
     1201 ccagcacgag acgcgctacg cgtacaccgc accggtgtcg cagtcgtggc agctcgcgcg
     1261 gctgacgccg cgcctgctgc cgtggcagcg gctgctggcg tactcgctgg agatcgagcc
     1321 cagggccgac gagcgccgcg acgaggtcga cagcttcggc aacatggtca cccacttcgg
     1381 cctgcacggc gcgcaccgca tgctgcgcgt gcgcatggag tgcgtggtcg aagtgaccga
     1441 gcggccggcg atcgacaccg ccgcggcgcc gatcgcgcag cagcagacgc agctccaggg
     1501 tgaggcggcg gagccgacgc agacgcagac acagacgcag gccgtcgcgg cggcgccgct
     1561 ggcctgggag gcggtgcgcg aggcgatccg gcgcgagccg cagcaggacg agctcgtccc
     1621 cgccagcatg agcgagccga cgccgctggt gccgctgtcc gaaggggcgc gccagtacgc
     1681 cgccgcgtcg ttcgcgcgcg gccggccgtg gctcgaggcg ctcaccgagc tgatgggccg
     1741 catccacgac gacttcgagt tcgagcccgg cgcgaccacc gtcgcgacct cggtcgacga
     1801 ggtgctgtac cagaagcgcg gcgtctgcca ggatttcgcg cacctgatgc tcgcctgtct
     1861 gcgcggccat ggcctgccgg cgcgctacgt gtcgggctac ctgctcaccg acccgccgcc
     1921 cggcatggcg cgtctgatgg gcgccgatgc gtcgcacgca tgggtcgccg cgtactcgcc
     1981 gcagcacggc tgggtcgagt tcgacccgac caacaaccag ctcgccgacc agcgctacat
     2041 cacgctgacc tggggcgccg atttcgccga cgtggtgccg ctgcgcggcg tcatcctggg
     2101 gggcggcgag cagcgcatgg aggtcgaggt cagtgtgatc ccggcctagg aggaagtcgg
     2161 tcgcggcgaa ggtggcggga gccgcgatct gcttgcgacc cagagcggtc cgacgagtca
     2221 ggaaggaagc agatgcccaa gcagcatggc ggcagcgccg tgaccgaggt ggaaggacct
     2281 tccttggggc tttgcgtcta cgacttggtg agaacgatgt
//
LOCUS       DATMJB010000493         2488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_434301, whole genome shotgun sequence.
ACCESSION   DATMJB010000493 DATMJB010000000
VERSION     DATMJB010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434301"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2145)
                     /gene="aceE"
                     /locus_tag="VNU48_10585"
     CDS             complement(<1..2145)
                     /gene="aceE"
                     /locus_tag="VNU48_10585"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HWI11766.1"
                     /translation="MSAQPDSFLGAAANDADAQETREWLDALSAVIGAEGGERAHFLL
                     ETLIDHARQAGIDVPFSANTAYVNTIPVDQEERTPGNIEIEERLRTYMRWNAMAMVVK
                     ANRLHPADGGDLGGHISSFASLATMFGTGFNHFWHAESEGHGGDLLYIQGHSSPGIYA
                     RAYMEGRISEEQLLNFRQEVDGKGISSYPHPKLMPEFWQFPTVSMGLGPLMAIYQARF
                     LKYLHARGIADTANRKVWVFCGDGEMDEPESLGAIGLAAREKLDNLIFVINCNLQRLD
                     GPVRGNGKIIQELEGEFRGAGWNVIKLIWGSYWDPLLARDKNEILRKVMMDTVDGDYQ
                     AMKANDGAFVRKHFFGQHPKLLEMVAKMSDEDIWRLNRGGHDPQKVYAAFHKAVNTAG
                     QPSVLLIKTDKGYGMGKSGEGKNTAHQTKKLIDEDVRAFRDRFNMPIPDDQLADIPFY
                     KPADDTPEMKYLHERRRALGGYLPHRRTKADEKLAVPPLETFKAVLDATAEGREISTT
                     QAFVRFLTQLLRDKELGPRAVPILVDEARTFGMEGLFRQIGIYNPAGQNYTPVDKDQV
                     MYYREDKAGQVLQEGINEPGGMSSWIAAATSYSTNNRIMVPVYVYYSMFGFQRVGDLA
                     WAAGDMQARGFLLGGTSGRTTLNGEGLQHEDGHSHILASTIPNCVSYDPAFAHEVGVI
                     LHHGLKRMVEQQDNVYFYLTLLNENYPQPGLRA"
BASE COUNT          346 a          823 c          834 g          485 t
ORIGIN      
        1 cgcgcgcagg ccgggctgcg ggtagttctc gttgagcaag gtcaggtaga agtagacgtt
       61 gtcctgctgc tcgaccatgc gcttcagccc gtggtgcagg atcacgccga cctcgtgcgc
      121 gaaggccggg tcgtagctga cgcagttggg gatcgtgctg gccaggatgt ggctgtggcc
      181 gtcctcgtgc tgcaggccct cgccgttgag cgtcgtgcgc cccgacgtgc cgccgagcag
      241 gaagccgcgc gcctgcatgt cgccggcggc ccacgcgagg tcgccgacgc gctggaagcc
      301 gaacatggag tagtagacgt agaccggcac catgatgcgg ttgttggtgc tgtagctcgt
      361 cgccgcggcg atccagctgc tcatgccgcc gggctcgttg atgccttcct gcaagacctg
      421 gccggccttg tcctcgcggt agtacatcac ctggtccttg tcgaccggcg tgtagttctg
      481 gcccgcgggg ttgtagatgc cgatctgacg aaacagcccc tccatgccga aggtgcgcgc
      541 ctcgtcgacg aggatcggca ccgcgcgcgg cccgagttcc ttgtcgcgca gcagctgcgt
      601 caggaagcgc acgaaggcct gcgtggtcga gatctcgcgg ccttcggcgg tcgcgtcgag
      661 caccgccttg aaggtctcga gcggcggcac cgccaatttt tcgtcggctt tcgtgcgccg
      721 atgcggcagg tagccgccga gtgcccggcg cctctcgtgc aggtacttca tctccggcgt
      781 gtcgtcggcc ggtttgtaga acgggatgtc ggcgagctgg tcgtcgggga tcggcatgtt
      841 gaagcggtcg cggaacgcgc gcacgtcctc gtcgatcagc ttcttggtct ggtgcgcggt
      901 gttcttgccc tcgccgctct tgcccatgcc gtagcccttg tccgtcttga tcagcagcac
      961 gctcggctgg ccggcggtgt tgacggcctt gtggaacgcc gcatagacct tctgcgggtc
     1021 gtgaccgccg cggttcaggc gccagatgtc ctcgtcgctc attttcgcga ccatctcgag
     1081 cagcttcgga tgctggccga agaaatgctt gcggacgaac gcgccgtcgt tggccttcat
     1141 cgcctggtag tcgccgtcga ccgtgtccat catgaccttg cgcaggatct cgttcttgtc
     1201 gcgcgcgagc agcgggtccc agtagctgcc ccagatcagc ttgatcacat tccagccggc
     1261 gccgcggaat tcgccctcga gctcctggat gatcttgccg ttgccgcgca ccgggccgtc
     1321 caggcgctgc aggttgcagt tgatgacgaa gatcaggttg tcgagcttct cgcgcgccgc
     1381 caggccgatc gcgcccagcg attcgggctc gtccatctcg ccgtcgccgc agaagaccca
     1441 gaccttgcgg ttcgcggtgt cggcgatgcc gcgtgcgtgc aggtacttca ggaagcgcgc
     1501 ctggtagatc gccatcagcg ggcccaggcc catcgagacg gtcgggaact gccagaactc
     1561 gggcatcagc ttcgggtgcg ggtagctcga aatgcccttg ccgtcgacct cctggcggaa
     1621 gttcagcagc tgctcctcgc tgatgcgccc ttccatgtag gcgcgcgcgt agatgcccgg
     1681 cgaagagtgg ccctggatgt acagcaggtc gccgccgtgg ccctcgctct cggcgtgcca
     1741 gaagtggttg aagccggtgc cgaacatcgt cgccagcgac gcgaagctgg agatgtggcc
     1801 gcccaggtcg ccgccgtcgg ccgggtgcag gcggttcgcc ttgaccacca tcgccatcgc
     1861 gttccagcgc atgtaggtac gcaggcgttc ctcgatctcg atgttgccgg gcgtgcgctc
     1921 ctcctggtcc accgggatcg tgttgacgta cgcggtgttg gccgagaacg gcacgtcgat
     1981 gccggcctgg cgcgcgtggt cgatcagggt ctcgagcagg aagtgggcgc gctcgccgcc
     2041 ttcggcaccg atgaccgccg acagcgcgtc gagccactcg cgggtttcct gcgcatccgc
     2101 atcgttggcg gcggcaccga ggaatgaatc aggctgtgct gacatgcttg tctcctgggg
     2161 tggatttgtg ggctgcgcgg gagtgtcgca caactcgccc gactttccaa atggtgcgag
     2221 tcgatttcac attatgattt ccaacggacg agatccctct gaccaccgtg cccgggttgg
     2281 acgcgcggcc cgggcccctg cgccaacctg ataatcccgc catgcaaccc gccaccgagg
     2341 ccgcaccgct gctccgcccc gcacgcacct ggcccgaaaa actgttcggc cgctggtggc
     2401 gccgccagtc cgccgcgcgt caggaccgct acgccacgct cgggccgctg gcctcggtgc
     2461 tgctgttcct cgcggccatc atcttggc
//
LOCUS       DATMJB010000494         4639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_523267, whole genome shotgun sequence.
ACCESSION   DATMJB010000494 DATMJB010000000
VERSION     DATMJB010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523267"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..666
                     /gene="ureE"
                     /locus_tag="VNU48_10590"
     CDS             106..666
                     /gene="ureE"
                     /locus_tag="VNU48_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HWI11767.1"
                     /translation="MLHVNKLLPAGRGLAPALLKRAAAVVLDWDTRQKSRFDTEDSQG
                     RTLGVFLPRGSTVRGGDVLVAEDGSLIKVIARPQAVMRITAGAARGAPFDLVRAAYHL
                     GNRHVAIELTPEHLQIEPDHVLAEMLRKMHLDVTEELAPFEPEAGAYASGGHDHGHAH
                     AAHAAAPHVHGPGCGHDHAHDHGHKH"
     gene            678..1391
                     /locus_tag="VNU48_10595"
     CDS             678..1391
                     /locus_tag="VNU48_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HWI11768.1"
                     /translation="MPRVRRAAPAGSAPALLQLMWLASPALPVGGFSYSEVLEAAVES
                     GHVRDEPQAAAWLLDQLQLSLARSDLAVVAKGFKAWSRGDFATITELNTWVTTTRESA
                     ELRQQTEQMGRSMVEWLKNRGGAVDARVAQLKALAPAPTWPVAFALAAAQTGAAPRDA
                     LLSFAFGWAENMVQAALKAVPLGQSAGQRILAALTGAIPAAVDHAAALMDSERQAFAP
                     MLAILSAQHELQYSRLFRS"
     gene            1397..1936
                     /locus_tag="VNU48_10600"
     CDS             1397..1936
                     /locus_tag="VNU48_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HWI11769.1"
                     /translation="MARNVEIKARIDSVEALLPRARACADADPERIAQDDTFFACASG
                     RLKLRVFADGRGELIAYERPDASGPKTSDYAITPVPDPDALRATLARALGVSGRVVKQ
                     RTLLRVGRTRVHLDRVAGLGDFLELEVALRDGESAAAGVSEAHALLARLQVDAAQLVS
                     GAYVDLLRLQWARPAAAPP"
     gene            1945..2592
                     /gene="ureG"
                     /locus_tag="VNU48_10605"
     CDS             1945..2592
                     /gene="ureG"
                     /locus_tag="VNU48_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HWI11770.1"
                     /translation="MSTALHQIANRTRKLPPLRVGVGGPVGSGKTTLVEMLCKSMRER
                     YDLVVVTNDIYTKEDQRLLTVAGALDAERIMGVETGGCPHTAIREDASINLEAVDRML
                     EKFPNADIVFIESGGDNLAATFSPELSDLTIYVIDVAAGEKIPRKGGPGITKSDLFVI
                     NKTDLAPHVGASLEVMAADTKRMRGNRPFVMSNLKTNAGLAEVVAFIETKGMLAA"
     gene            complement(2600..3664)
                     /locus_tag="VNU48_10610"
     CDS             complement(2600..3664)
                     /locus_tag="VNU48_10610"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HWI11771.1"
                     /translation="MSATLFRNLLASGVLVLSGLSVAPGALAQGRPPTTEELGKDPGA
                     LRQLEEQRNAQQLRQQQGQQQQQQDQLYNDALRQQQARQQGDIAQGQAVLRSWQQRPP
                     LAAAQNPLLGRWNSLGSAGAKRSAPGVSPEMAQLVNGLLGGITGGLCDSMLGRGLVEF
                     RPTAVVAIGRDGRERPMYRADYRGGGSRVVVLPQPGQSFTHMIIDFNGPDRATVAAVG
                     CVLVRAGGGAAAATGEAVAAPAELQWVLLGTSAANGGMDIYVARSTIRRAGHSAQMWD
                     LWDFKTDHRFEGKRFLSARNQYEYDCASTRRRMLSTVGYSGHMGQGAVVGSGGAMLAW
                     EPVGASGPIRDYWNIACAKS"
     gene            complement(3773..>4639)
                     /gene="sppA"
                     /locus_tag="VNU48_10615"
     CDS             complement(3773..>4639)
                     /gene="sppA"
                     /locus_tag="VNU48_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HWI11772.1"
                     /translation="RSDRRWRIFFRLSWLLLVMAIAYAAFAVRNRAATPTGPHTALIE
                     LRGEIAAGAEASAENLIPGIKAAFEDDSAKAIVLRINSPGGSPVQAGIINDEIRRLRK
                     LHPKKVYAVVEETCASGAYYIAVAADEIYADKASVVGSIGVLMDSFGAAGLMDKLGVE
                     RRLITAGENKGLGDPFSPLLPKQRAYLQGMIDQIHQQFIAVVKQGRGKRLKETPETFT
                     GLFWNGEQALAMGLIDHLGNLDYVAREVVKAEEVIDYTPKENVAERLAKKFGASIGAG
                     AVKAIPGAFTIR"
BASE COUNT          666 a         1693 c         1631 g          649 t
ORIGIN      
        1 cgggcgtcgg ggccaggccg tgggcggcgc gacgcgacgc gacgcgaacg cagcgcgaaa
       61 cgcggcgctg gcacactagg gcctgtcatc gaccggaccg cccccatgct gcacgtcaac
      121 aagcttcttc ccgccggccg cgggctggcc cccgcgctgc tgaagcgcgc cgccgcggtc
      181 gtgctcgact gggacacgcg ccagaagagc cgcttcgaca ccgaggactc gcagggccgc
      241 acgctcggcg tgttcctgcc gcgcggcagc acggtgcgcg gcggcgacgt cttggtggcc
      301 gaggacggct cgctgatcaa ggtcatcgca cggccgcagg cagtgatgcg catcaccgcc
      361 ggcgccgcgc gcggcgcgcc cttcgatctg gtgcgcgccg cctaccacct gggcaaccgc
      421 cacgtggcga tcgagctgac gcccgagcac ctgcagatcg agcccgacca cgtgctcgcc
      481 gagatgctga ggaagatgca cctggacgtg accgaagagc tcgcgccctt cgagccggaa
      541 gccggcgcct acgccagcgg tggccacgac catggccacg cgcacgccgc gcacgccgcc
      601 gcgccgcacg tgcacgggcc gggctgcggc cacgatcacg cgcacgatca cggccacaag
      661 cactgagccg cccgatcatg ccgcgcgttc gccgcgccgc gccggccggc tccgcgccgg
      721 cgctgctgca gctgatgtgg ctggcctcgc cggccttgcc ggtgggcggc ttcagctact
      781 ccgaggtgct cgaggccgcg gtcgagtcgg gccacgtgcg cgacgaaccg caggccgctg
      841 cgtggctgct cgatcagctg cagctgagcc tggcgcgcag cgatctcgcg gtggtggcga
      901 agggcttcaa ggcctggagc cgcggcgact tcgcgacgat caccgagctg aacacctggg
      961 tcacgaccac gcgcgaatcc gccgaactgc gccagcagac cgaacagatg ggccgctcga
     1021 tggtcgagtg gctgaagaac cgcggcggcg cggtcgatgc acgcgtcgcg caactgaagg
     1081 cgctcgcgcc ggcgccgacc tggccggtcg cgttcgcgct cgccgcggcg cagaccggcg
     1141 cggcgccgcg cgacgcgctg ctgagcttcg cgttcggctg ggccgagaac atggtgcagg
     1201 cggcgctgaa ggcggtgccg ctcgggcaga gcgcggggca gcgcatcctg gccgcgctga
     1261 ccggggcgat tcccgccgcc gtcgaccacg cggccgcgct gatggacagc gaacgccagg
     1321 ccttcgcgcc gatgctcgcg atcctgtcgg cacagcacga gctgcagtac tcgcgcctgt
     1381 tccgctcctg atcgcgatgg cgcgcaacgt cgagatcaag gcccgcatcg acagcgtcga
     1441 ggcgctgctg ccgcgcgccc gcgcctgcgc cgacgccgac cccgagcgca tcgcgcagga
     1501 cgacaccttc ttcgcgtgtg ccagcgggcg cctgaagctg cgcgtgttcg ccgacggccg
     1561 cggcgagctg atcgcctacg agcggcccga cgcgagcggg cccaagacct cggactacgc
     1621 gatcacgccg gtgcccgacc cggacgcgct gcgcgccacg ctggcgcgcg cgctcggcgt
     1681 cagcggccgg gtcgtcaagc agcgcacgct gctgcgcgtg ggccgcacgc gcgtgcacct
     1741 ggaccgggtc gccggcctgg gtgactttct cgaactcgag gtcgcgctgc gcgacggcga
     1801 gagcgcggcg gccggcgtct cggaggccca tgcgctgctg gcgcggctgc aggtcgatgc
     1861 cgcgcagctc gtgtccgggg cctatgtcga tctcttgcgc ctgcagtggg cgcggcctgc
     1921 agccgcgccg ccataatcca tgcgatgagc accgccctgc accagatcgc gaaccgcacc
     1981 cgcaagctgc cgccgctgcg cgtcggcgtc ggcgggccgg tcggctcggg caagacgacg
     2041 ctggtcgaga tgctgtgcaa gtcgatgcgc gagcgctacg acctggtcgt cgtcacgaac
     2101 gacatctaca ccaaggaaga ccagcgcctg ctgaccgtgg ccggcgcgct cgacgccgag
     2161 cgcatcatgg gcgtggagac cggcggctgc ccgcacaccg cgatccgcga ggatgcgtcg
     2221 atcaacctcg aggcggtcga ccgcatgctc gagaagttcc cgaacgccga catcgtgttc
     2281 atcgagagcg gcggcgacaa cctcgcggcg accttcagcc ccgagctcag cgacctgacg
     2341 atctacgtga tcgacgtcgc cgccggcgag aagatcccgc gcaagggcgg cccgggcatc
     2401 acgaagagcg acctgttcgt gatcaacaag accgacctcg cgccgcacgt cggcgcgagc
     2461 ctcgaggtga tggcggccga cacgaagcgc atgcgcggca accggccctt cgtgatgagc
     2521 aacctgaaga cgaacgccgg gctggccgag gtcgtggcct tcatcgagac caagggcatg
     2581 ctcgccgcct gatgcgcgct caggacttcg cgcaggcgat gttccagtag tcgcggatcg
     2641 ggccgctggc gccgaccggc tcccaggcga gcatcgcgcc gcccgagccg acgaccgcac
     2701 cctggcccat gtgcccggaa tacccgacgg tggacagcat gcgccgccgc gtgctggcgc
     2761 agtcgtactc gtactggttg cgggccgaca ggaagcgctt gccctcgaac cggtggtcgg
     2821 tcttgaagtc ccacaggtcc cacatctgcg ccgaatgccc cgcgcggcgg atcgtcgaac
     2881 gggcgacgta gatgtccatg ccgccgttcg ccgccgacgt gcccagcagc acccattgca
     2941 gctcggcggg tgcggccacc gcttcgccgg tggcggccgc ggcaccgccg ccggcacgca
     3001 ccagcacgca gcccaccgcc gcgacggtgg cgcggtccgg gccgttgaag tcgatgatca
     3061 tgtgggtgaa ggattgcccg ggctgcggca acacgacgac gcgcgaaccg ccgccgcggt
     3121 agtcggcgcg gtacatcggc cgctcgcgcc cgtcgcggcc gatcgccacc acggcggtcg
     3181 ggcggaactc gaccagcccg cggccgagca tcgagtcgca caggccgcca gtgatgccgc
     3241 ccagcagccc gttcacgagc tgcgccatct cggggctgac gcccggcgct gatcgcttcg
     3301 ccccggctga gccgagcgaa ttccagcgcc cgagcagcgg gttttgggcc gccgccagcg
     3361 gcgggcgctg ctgccagctg cgcagcacgg cctggccctg cgcgatgtcg ccttgttgcc
     3421 gcgcctgctg ctggcgcagc gcgtcgttgt acagctggtc ctgctgctgt tgctgctgcc
     3481 cctgctgctg gcgcagctgc tgggcattgc gctgctcttc gagctggcgc agcgcgccgg
     3541 gatccttgcc gagctcctcg gtcgtcggcg gacgcccctg cgccagcgcg cccggcgcga
     3601 cgctcaggcc cgacaacacc agcacgccgc tggcaagcag gttgcggaac aaggttgcgc
     3661 tcatcgccga ctcctcgacg cgcgttcggc gccccacggg cacgcgaggt cacgcgccgc
     3721 cagcgtagtc ggcccggcgt taccgcagcg ttaccgcagg ggacgcgcgc gttcagcgaa
     3781 tcgtgaacgc gcccggaatc gccttgaccg cgccggcgcc gatcgatgca ccgaacttct
     3841 tcgccaggcg ttcggcgacg ttttccttgg gcgtgtagtc gatcacctct tcggccttga
     3901 cgacctcgcg cgcgacgtag tcgaggttgc ccaggtggtc gatcaggccc atcgccagcg
     3961 cctgttcgcc gttccagaac aggcccgtga aggtctccgg cgtttccttc agccgcttgc
     4021 cacggccctg cttgacgaca gcgatgaact gctgatggat ctgatcgatc atcccctgca
     4081 ggtaggcgcg ctgcttcggc agcagcggcg agaacgggtc gcccaggccc ttgttctcgc
     4141 ccgcggtgat caggcggcgc tcgacgccga gcttgtccat cagcccggcc gcgccgaagc
     4201 tgtccatcag cacgccgatc gagccgacca ccgatgcctt gtcggcgtag atctcgtcgg
     4261 ccgcgaccgc gatgtagtag gcgccggagg cgcaggtctc ttcgacgacg gcgtagacct
     4321 tcttcgggtg cagcttgcgc aggcgccgga tctcgtcgtt gatgatgccg gcctgcaccg
     4381 ggctgccgcc gggcgagttg atgcgcagca cgatcgcctt cgcgctgtcg tcctcgaacg
     4441 cggccttgat gccgggtatc aggttctcgg cgctggcctc ggcgcccgcg gcgatctcgc
     4501 cacggagctc gatcagcgcg gtgtgcgggc cggtcggtgt cgcggcgcgg ttgcgcaccg
     4561 caaaggccgc gtaggcgatc gccatcacca gcagcagcca cgacaggcgg aagaagatgc
     4621 gccagcggcg gtcgctgcg
//
LOCUS       DATMJB010000495         2428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_215416, whole genome shotgun sequence.
ACCESSION   DATMJB010000495 DATMJB010000000
VERSION     DATMJB010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215416"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VNU48_10620"
     CDS             complement(<1..366)
                     /locus_tag="VNU48_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWI11773.1"
                     /translation="MPWVFVLIWSTGFVVARFGMPHSPPLTFLSIRYALSLLCFGVWI
                     RVARVAWPRERVQWAHLAVVGVLIHAGYLGGVWAAVKAGIGAGTVALLVGLQPVLTAL
                     WMSARARPAGDARAGVSRQQ"
     gene            complement(411..977)
                     /gene="pgsA"
                     /locus_tag="VNU48_10625"
     CDS             complement(411..977)
                     /gene="pgsA"
                     /locus_tag="VNU48_10625"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HWI11774.1"
                     /translation="MFFNLPTMLTWARIVAIPLIVGVFHLPLAEADRNLIATVLFIVV
                     ALTDWADGYLARRLNMTSSFGAFLDPVADKFLVCAALLVLVELERVQSLVALIIIGRE
                     IAISALREWMAQIGASRSVAVHMLGKVKTVVQMVAIPFLLFHGALFGVIDTQRWGTWL
                     IYASAVLTIWSMVYYLQKALPEIRAKAR"
     gene            complement(1032..1523)
                     /locus_tag="VNU48_10630"
     CDS             complement(1032..1523)
                     /locus_tag="VNU48_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11775.1"
                     /translation="MRCIDRRWLRAALGGLGLIAGCSSAPPPAPIATAPSVASTATNA
                     AASVRLPPPGPVRSWAELRLQAAQRLVAANPNGSYTGEVIEPLLGIPVLEIELNGDGS
                     VRRIVFLRHAQEAKDTSQVAADAVHRAAPFGDVSRLPKPWRFTETFLFNHERKFKPRT
                     LDP"
     gene            complement(1510..>2428)
                     /locus_tag="VNU48_10635"
     CDS             complement(1510..>2428)
                     /locus_tag="VNU48_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWI11776.1"
                     /translation="VPPLLVLSHAVDKTLIDALSLHSGIKVTAQHQPREPRRSWLDMC
                     IKGAQLKLAQLLAEEGSQHARTRALVEALDLAVDDMDTFRIECFDISHTAGESTQASC
                     VVFEGHKMQSAQYRRYNIEGITGGDDYAAMRQVLTRRYAKLVEAASRADVPNSARLPD
                     LVLVDGGRGQVSMAREVFEELGIDLGRIVGVEKGEGRKVGLEELVFADGRPKVHLGRD
                     SAALMLVAQIRDEAHRFAITGMRARRASVRTGGSKLEDIAGIGPKKRAKLLQRFGGVR
                     GVGDASVQDLLSVDGISKELAEEIYRALH"
BASE COUNT          402 a          872 c          817 g          337 t
ORIGIN      
        1 ctgctgccgc gacacgccgg cgcgcgcgtc gcccgcaggg cgcgcgcgcg cgctcatcca
       61 caacgccgtc agcaccggct gcaggcccac cagcagcgca acggtgccgg cgccgatacc
      121 ggccttcacc gcggcccaca cgccgccgag gtagccggcg tggatcagca caccgacgac
      181 cgccagatgc gcccactgca cgcgctcgcg cggccacgcg acgcgcgcaa cgcggatcca
      241 cacgccgaag cacagcagcg acaaggcata gcggatcgac aggaaggtca gcggcggcga
      301 atgcggcatg ccgaagcgcg cgaccacgaa gccggtgctc cagatcagca cgaacaccca
      361 cggcatcgcg cggatcagcg catcgcgccg gtcgatcgcg gcggccgaca ctagcgggct
      421 ttggcgcgga tctcgggcag cgccttctgc aggtagtaca ccatcgacca gatcgtcaac
      481 accgccgacg cgtagatcag ccacgtgccc cagcgctgcg tgtcgatcac accgaacagc
      541 gcgccgtgga acagcaggaa cgggatcgcc accatctgca ccaccgtctt gaccttgccg
      601 agcatgtgca ccgcgacgct gcgcgacgcg ccgatctgcg ccatccattc gcgcagcgcc
      661 gagatcgcga tctcgcgccc gatgatgatg agcgcgacca ggctctgcac gcgttcgagc
      721 tcgaccagca cgagcagcgc cgcgcagacc aggaacttgt cggccaccgg atcgaggaag
      781 gcgccgaacg acgaggtcat gttcaggcgc cgcgcgaggt agccgtcggc ccagtcggtg
      841 agtgcgacga cgatgaacag cacggtggcg atcaggttgc gatcggcctc ggccagcggc
      901 agatgaaaca ccccgacgat cagcggaatc gcgacgatgc gcgcccaggt cagcatggtc
      961 ggcaggttga agaacatgcg gcgattgtga cccgacttgt ggagtcctcg cccgcgcagt
     1021 gccgcgagcc gctaggggtc gagcgtgcgc ggcttgaact tgcgctcgtg attgaacagg
     1081 aaggtctcgg tgaagcgcca gggcttgggc agccgcgaca cgtcgccgaa gggcgcggcg
     1141 cggtgcaccg cgtcggcggc gacctgcgag gtgtccttcg cctcctgcgc gtggcgcagg
     1201 aacacgatcc tgcgcacgct gccgtcgccg ttgagctcga tctcgagcac cgggatgccc
     1261 agcagcggct cgatcacctc gccggtgtag ctgccgttcg ggttcgcggc aacgagacgt
     1321 tgcgcggcct gcagccgcag ctcggcccag ctgcgcaccg ggcccggcgg cggcagccgc
     1381 accgaggcgg ccgcattggt ggccgtcgac gcgacgctcg gcgccgttgc gatcggtgcc
     1441 ggtggcggcg cagacgagca gccggcgatc aggcccagcc cgccgagtgc cgcacgcagc
     1501 catcgacgat caatgcagcg cacgatagat ctcctcggcg agttccttcg agatgccgtc
     1561 gacgctcaac agatcctgca cgctcgcgtc gccgaccccg cgcacgccgc cgaagcgctg
     1621 cagcagcttc gcgcgcttct tcggcccgat gccggcgatg tcctccagct tgctgccgcc
     1681 ggtgcgcacg cttgcgcgcc gcgcgcgcat gccggtgatc gcgaagcgat gcgcctcgtc
     1741 gcggatctgc gcgaccagca tcagcgccgc cgaatcgcgg ccgaggtgga ccttgggccg
     1801 accatccgcg aacaccagtt cttcgagccc gaccttgcgg ccctcgccct tctcgacgcc
     1861 gacgatgcgg ccgagatcga tgcccaattc ctcgaagacc tcgcgcgcca tgctgacctg
     1921 gccgcgcccg ccgtcgacca gcaccaggtc gggcagacgc gccgaattcg gaacatcggc
     1981 acggctcgcg gcctcgacaa gcttggcata gcggcgcgtc agcacctggc gcatcgcggc
     2041 atagtcgtcg ccgccggtga tgccctcgat gttgtagcgg cggtactggg cactctgcat
     2101 cttgtggcct tcgaagacga cacaggatgc ctgggtcgac tcgccggccg tgtggctgat
     2161 gtcgaaacac tcgatccgaa aggtgtccat gtcgtccacc gcgaggtcca gcgcctcgac
     2221 cagcgcgcgg gtccgcgcgt gctgcgagcc ctcttcggcg agcagctgcg cgagcttgag
     2281 ctgcgcgccc ttgatgcaca tgtccagcca cgagcgccgc ggctcgcgcg gctggtgctg
     2341 cgcggtcacc ttgatgccgc tgtgcagcga cagggcgtcg atcagcgtct tgtcgaccgc
     2401 gtggctgagc accagcagcg gcggtacg
//
LOCUS       DATMJB010000496         8038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_652369, whole genome shotgun sequence.
ACCESSION   DATMJB010000496 DATMJB010000000
VERSION     DATMJB010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652369"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VNU48_10640"
     CDS             <1..368
                     /locus_tag="VNU48_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWI11777.1"
                     /translation="KGRPVLLNFWATWCEPCRAEMPSLELLATRHERAGLAILAVNYQ
                     EPLPAIKRFLETLPFSLTILLDRDGDAASAWTPRVFPTTVLIDRDGRARSSVIGELDW
                     MGETARALVEPLLAHPKAA"
     gene            518..1324
                     /gene="lepB"
                     /locus_tag="VNU48_10645"
     CDS             518..1324
                     /gene="lepB"
                     /locus_tag="VNU48_10645"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWI11778.1"
                     /translation="MSVQLGFSINEILALATLATGACWVAWKLRKRRLGREPEKKPLW
                     VSFGAELFIVVALMFTCRIAVADWPKVPSGSMEPTLRVGDYLLVNHLAYGPRLPFTNT
                     AIEFGQPKRGDVVVFRYPVDVSEFYVKRLIGLPGDVVRFEGGVVSVNGAPFDAKVVSE
                     GVGANVVPEDRGQWLLHETSGGNSRTVKLNPFVLGRLAPSGMGSACAVEGPTAWQCTV
                     PAGEYLMMGDNRDNSADSRVWGFLPHEEVYGKAVRVLFNLHDMSRLWTAL"
     gene            1321..2553
                     /gene="coaBC"
                     /locus_tag="VNU48_10650"
     CDS             1321..2553
                     /gene="coaBC"
                     /locus_tag="VNU48_10650"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HWI11779.1"
                     /translation="MNASTSPALDLAGRHIVLGLSGGIACYKAADFTRELVKAGATVQ
                     VVMTEAAERFITPVTLQALSNRPVFTSQWDAREANNMAHINLTRGADAIVVAPASADL
                     MARLAQGRADDLLSLMCLARPIERCPLLLAPAMNREMWAHPATQRNLAQLRADGTVIL
                     GPGSGDQACGEIGDGRMLEPAELLAELVAFFQPKLLAGKRVLITAGPTFEPIDPVRGL
                     TNRSSGKMGFAVARAAAEAGAAVTLVAGPVHLPTPRGVTRIDVITARQMFDAATSAAP
                     SNDVFVATAAVADWRPAAFNDHKIKKRPGVKTPPSFELTENPDILAAIARLDNAPYCV
                     GFAAESHDLLQHARDKLERKGVPLIVANLGPATFGQDDNLLTLVDASGALELPRADKL
                     ALARTLVAEIARRLGARA"
     gene            2550..2999
                     /gene="dut"
                     /locus_tag="VNU48_10655"
     CDS             2550..2999
                     /gene="dut"
                     /locus_tag="VNU48_10655"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HWI11780.1"
                     /translation="MTAIDLKLLDPRLAEQLPAYATPGSAGLDLRACVDAALVIEPGQ
                     TVLIPTGMAIHLGDPGLAAMILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVSCWNRG
                     AAAFTVQPLDRIAQLVIVPVVQAAFRVVPEFGASDRGAGGFGSTGRA"
     gene            3130..4272
                     /locus_tag="VNU48_10660"
     CDS             3130..4272
                     /locus_tag="VNU48_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWI11781.1"
                     /translation="MSRALTGWAIAVVAAIGAAGCGKGPATDAASTAAAPASAAPVSI
                     SSVRALTKDVDVVLEATGTISALNSVDIRPQVSSVITQVNIREGQFVKAGQLLFTLDA
                     RNDEVNLAKARAQLARDLASLADAQRQLQRSRDLLAQNFISQGAVDTNQTLVDTQQAV
                     VTADRAAIDAAQVGLSYTRIVAPAAGRAGAINVFAGSTVQPGGAALVTITQLDPIAVS
                     FGLPQRHLNEALQTLRAGGGKVFAVLPDGRGEVVGKLQFVDNVVDANSGTVKVKAVFD
                     NAHELLWPGGFVNVRLATRTLKDAIVVPQAAVIRGPRGALVYTVDADSRAVARPVELL
                     HAAGLEAAIRGIQPGERVVLDGRQNLRAGVPVVERAASGSANGAVP"
     gene            4269..7367
                     /locus_tag="VNU48_10665"
     CDS             4269..7367
                     /locus_tag="VNU48_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWI11782.1"
                     /translation="MNLSEPFIRRPVLTVLLNAAIVVAGVIAYGKIPIAALPSYNTPV
                     INVFAALPGASPETMATAVALPLEKQFATIPGLNVISSSNTLGASSLTLEFDEGRNID
                     AASVDVQAALLRAQRVLPQDMTTPPSYRKVNPADAPVLFMALTSPSLNLAELNDFAEH
                     LISPTLATLDGVAQVTIFGAKRFAVRVRVQPQALAARNIGLDELSAALTAANVNTPVG
                     TLEGPQQTLTLQANRQLRNAAEFAELIVSNRAGGPVRLRDVASVEDSYESVKTFASFD
                     GEHAISLAVFRQPDANTVKVVDAVRAAMPGFRSQLPHSVRVSLVNDRSVSVRAALHDV
                     TLTMFGTIVLVVLVIFLFLRRFMATVIPTLSLPVSLLGAIALLGGFGYTLDNISLLGI
                     TLAVGLVVDDAIVMLENIMRHVENGEPPFQAALRGSREVGFTILSISTSLIAVFIPIF
                     FMPGVIGLLFHEFAVVVSLAIIASAFVSLTLVPMMAARFLSDRAHRKPPGLVVRAFER
                     GFDALLAGYTRSLDAALRHRGFIGCVAVLTLAATAWLFVTIPKGFFPEEDIGQIRVTT
                     EAAEDTSFPAMVQLQERIADIFRADPNVASVNSFNGGDGAQNTGRLFLNLKPRAERAP
                     MKEVVEGLRRKLRDVPGITVFMRPIQNLQLGGRQSKAQYQYILQSVSAAELSDWATRL
                     QDAMRVDPLFRDVTSDSQLKGLQASLKIDRERANTLGVSIDAVRNALYSAFGERQVST
                     IYTSVDSYQVIMEVAPEAKQDESAFSAIFVRSSNGTLVPLSAFTAVERTIGPTSVNHA
                     GQLQAVTVSFNLAPGAALGDATAKLERYRDQIRMPAAIVTSYGGDAAVFKSSQGSQVI
                     LIIGALLVIYVLLGVLYESYVHPLTILAGLPSAAVGALLMLRLFDMDLTLIATIGILL
                     LIGIVKKNAIMMIDFALDAQRTRGAAPAAAIREACVLRFRPIMMTTLAALMGALPIAL
                     GLGAGAELRQPLGLAVVGGLVFSQAITLYITPVIYLALDRFSGTGPVTTPEQPAAVKK
                     SG"
     gene            complement(7474..7995)
                     /locus_tag="VNU48_10670"
     CDS             complement(7474..7995)
                     /locus_tag="VNU48_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWI11783.1"
                     /translation="MPITTPCVVSLTWKLQDTQGSLIDELQDPVEFFYGGDDLLAKVE
                     EALAGQTAGFTTTLHLEPEHAFGDYDPALVFFEARSVFSEVIEVGMQFDGLPPGATTQ
                     GMPADAIYTVTEVYPAHVVLDGNHPLAGIALRLDLTVRDVRAATAEEVEAGSVGQPVF
                     TVMNTAPSGSALH"
BASE COUNT         1171 a         2812 c         2863 g         1192 t
ORIGIN      
        1 tcaagggccg cccggtgctg ctgaacttct gggccacctg gtgcgaaccc tgccgcgccg
       61 aaatgccgtc actcgaactg ctggccacgc gccacgagcg cgccgggctg gcgatcctcg
      121 cggtcaacta ccaggagccg ctgcccgcga tcaagcgttt tctcgagacg ctgccgttct
      181 cgctgaccat cctgctcgac cgcgacggcg acgccgcgag cgcctggacg ccgcgcgtgt
      241 tcccgaccac ggtgctgatc gaccgcgacg gccgcgcgcg cagctcggtg atcggcgagc
      301 tcgactggat gggcgagacc gcgcgggcgc tggtcgagcc gctgctcgcg caccccaagg
      361 ccgcctgacg gcagcgccgg ctcgtggccg catgccgccc cgataatccc tcgtctgttt
      421 gctatggagt tgagagcatg gccacactga aacggcgcga ttcgctggcg ccgacgccga
      481 gcgcagcggc ggctgccggc gcacgggagt cgacggcatg agcgtgcagc tcgggttttc
      541 gatcaacgag attctcgcgc tcgccaccct cgcgaccggc gcctgctggg tcgcatggaa
      601 gctgcgcaag cgccgcctcg gccgcgagcc cgagaagaag ccgctgtggg tcagcttcgg
      661 ggccgagctg ttcatcgtcg tcgcgctgat gttcacctgc cgcatcgcgg tggccgactg
      721 gcccaaggtg ccgtcgggct cgatggagcc gaccctgcgc gtcggcgact acctgctcgt
      781 caaccacctc gcctacgggc cgcggctgcc gttcacgaac accgcgatcg agttcggcca
      841 gcccaagcgc ggcgacgtgg tcgtgttccg ctacccggtc gacgtgtcgg agttctacgt
      901 caagcgtctg atcggcctgc ccggcgacgt ggtgcgcttc gagggcggcg tggtcagcgt
      961 caacggcgcg ccgttcgacg ccaaggtcgt cagcgaaggg gtcggcgcga acgtcgtgcc
     1021 cgaggatcgc ggccagtggc tgctgcacga gaccagcggc ggcaacagcc gcaccgtcaa
     1081 gctcaacccg ttcgtgctgg ggcgcctggc gccgagcggc atgggcagcg cctgcgccgt
     1141 cgaaggcccg accgcctggc agtgcaccgt gccggccggc gagtacctga tgatgggcga
     1201 caaccgcgac aactccgccg attcgcgcgt ctggggtttc ctgccgcacg aagaggtcta
     1261 cggcaaggcg gtgcgggtgc tgttcaacct gcacgacatg tcgcgcctct ggaccgcgct
     1321 gtgaacgcgt cgacgagccc ggcgctcgat ctcgcgggca ggcacatcgt gctcggcctg
     1381 tccggcggca tcgcctgcta caaggcggcc gacttcacgc gcgagctggt gaaggcgggc
     1441 gcgaccgtgc aggtcgtgat gaccgaagcg gccgaacgct tcatcacgcc ggtgacgctg
     1501 caggcgctgt cgaaccgccc cgtgttcacg agccagtggg acgcgcgcga agcgaacaac
     1561 atggcgcaca tcaacctcac gcgtggtgcc gacgcgatcg tcgtcgcgcc cgccagcgcc
     1621 gacctgatgg ccaggcttgc gcagggccgt gccgacgacc tgctgagcct gatgtgcctg
     1681 gcgcggccga tcgaacgctg cccgctgctg ctggcgccgg cgatgaaccg cgagatgtgg
     1741 gcgcacccgg cgacgcagcg caatctcgcg cagctgcgcg ccgacggcac ggtgatcctc
     1801 ggccccggca gcggcgacca ggcctgcggc gagatcggcg acggccgcat gctcgaaccg
     1861 gccgagctgc tcgccgagct ggtcgcgttc ttccagccca agctgctggc cggcaagcgc
     1921 gtgctgatca ccgccggccc gaccttcgaa ccgatcgacc cggtgcgcgg cctgacgaac
     1981 cggtcgagcg ggaagatggg tttcgcggtc gcgcgcgccg ccgccgaggc gggcgctgcg
     2041 gtgacgctgg tcgccggccc ggtgcatctg ccgacgccgc gcggcgtgac gcgcatcgat
     2101 gtgatcaccg cgcgccagat gttcgatgcc gcgacgagcg cggcaccatc gaacgacgtg
     2161 ttcgtcgcca ccgccgcggt ggccgactgg cggcccgccg cgttcaacga tcacaagatc
     2221 aagaagcggc ccggcgtgaa gacaccaccg agcttcgagc tgaccgagaa cccggacatc
     2281 ctcgcggcga tcgcgcggct cgacaacgcg ccgtactgcg tcggcttcgc ggccgaaagc
     2341 cacgacctgc tgcagcacgc gcgcgacaag ctcgagcgca agggcgtgcc gctgatcgtc
     2401 gcgaacctcg gcccggcgac cttcgggcag gacgacaacc tgctgaccct ggtcgacgcg
     2461 agcggcgcgc tcgaactgcc gcgcgccgac aagctcgcgc tggcgcgcac gctggtcgcc
     2521 gagatcgcgc gccggctcgg agcgcgcgca tgacggcgat cgacctgaag ctgctcgacc
     2581 cgcgcctggc cgagcagttg ccggcctatg cgacgccggg cagcgcgggg ctggacctgc
     2641 gcgcctgcgt cgatgcggcg ctcgtcatcg aacccggaca gaccgtgctg atcccgaccg
     2701 gcatggccat ccacctcggc gacccgggcc tggccgcgat gatcctgccg cgctcgggcc
     2761 tgggccacaa gcacggcatc gtgctcggca acctggtcgg gctgatcgac tccgattacc
     2821 agggcccgct gatggtgagc tgctggaacc gcggcgcggc cgcgttcacc gtgcagccgc
     2881 tggaccgcat cgcgcagctc gtgatcgtgc cggtggtgca ggcggcgttc cgggtcgtgc
     2941 cggaattcgg cgcgtcggac cgcggcgccg gcggcttcgg atcgaccggc cgcgcctgag
     3001 cgccgccgcc cgccccgccc ccgcgggagt ggggtcgtta cgcgttgaag cgcgcctgtc
     3061 gaatggcgag aatccagcga gcaacgaagc tttgcaatcc gcaaggaagc gcgttcagtt
     3121 gggatccaca tgagcagagc gttgacgggt tgggcgatcg cggtcgtggc cgcgatcggc
     3181 gcggcggggt gcggcaaagg gcccgccacc gacgccgcgt cgaccgcggc ggcgccggcc
     3241 tcggccgcgc cggtcagcat cagcagcgtg cgcgcgctga cgaaggatgt cgacgtggtg
     3301 ctcgaggcca ccggcacgat cagcgcgctc aacagcgtcg acatccggcc gcaggtcagc
     3361 agcgtgatca cgcaggtcaa catccgcgag ggccagttcg tgaaggccgg gcagttgctg
     3421 ttcacgctcg acgcgcgcaa cgacgaggtc aacctcgcca aggcccgcgc ccagcttgcc
     3481 cgcgacctcg cctcgctggc cgacgcgcag cgccagctgc agcgcagccg cgacctgctg
     3541 gcgcagaact tcatctcgca gggcgcggtc gacacgaacc agacgctggt cgacacgcag
     3601 caggcggtgg tgaccgcgga ccgcgccgcg atcgacgcgg cgcaggtcgg cctgtcgtac
     3661 acccgcatag tcgcgccggc cgcgggccgg gccggtgcga tcaacgtgtt cgccggcagc
     3721 accgtgcagc ccggcggcgc ggcgctggtg acgatcacgc agctcgatcc gatcgcggtc
     3781 agtttcggcc tgccgcagcg ccacctgaac gaagcgctgc agactctgcg cgccggcggc
     3841 ggcaaggtct tcgcggtgct gcccgacggg cgcggcgagg tcgtcggcaa gctgcagttc
     3901 gtcgacaacg tcgtcgatgc gaattccggc acggtcaagg tcaaggcggt gttcgacaac
     3961 gcccacgagt tgctgtggcc gggcgggttc gtcaacgtgc gcctggccac ccgcacgctg
     4021 aaggacgcga tcgtcgtgcc gcaggcggcg gtgatccgcg ggccgcgcgg cgcgctggtc
     4081 tacaccgtcg atgcggacag ccgcgcggtg gcgcggccgg tcgagctgct tcatgccgcc
     4141 gggctggagg cggcgatccg cggcatccag ccgggcgagc gcgtcgttct cgacgggcgg
     4201 cagaacctgc gcgccggcgt gccggtcgtc gagcgcgcgg ccagcggcag cgccaacggg
     4261 gcggtgcctt gaacctgtcc gagccgttca tccgccggcc ggtgctgacg gtgctgctga
     4321 atgcggcgat cgtcgtcgcc ggcgtgatcg cctacggcaa gatcccgatc gcggcgctgc
     4381 cgagctacaa cacgccggtc atcaacgtct tcgccgcgct gcccggcgcg agccccgaga
     4441 cgatggcgac cgcggtggcg ctgccgctgg agaagcagtt cgcgaccatc ccggggctga
     4501 acgtgatcag ctccagcaac acgctgggcg cgagctcgct gacgctggag ttcgacgaag
     4561 gtcgcaacat cgacgccgcc tcggtcgacg tgcaggcggc gctgctgcgc gcacagcgcg
     4621 tgctgccgca ggacatgacg acgccgccct cgtaccgcaa ggtcaacccg gccgatgcgc
     4681 cggtgctgtt catggcgctg acctcgccgt cgctgaacct ggcggagctg aacgacttcg
     4741 ccgagcacct gatctcgccg acgctggcga cgctggacgg cgtggcgcag gtcacgatct
     4801 tcggcgcgaa gcgtttcgcg gtgcgcgtgc gggtgcagcc gcaggcgctg gcggcgcgca
     4861 acatcggcct ggacgagctc agcgccgcgc tcaccgcggc caatgtcaac acgccggtcg
     4921 gcacgctcga agggccgcag cagacactga cgctgcaggc gaaccggcag ttgcgcaacg
     4981 ccgccgagtt cgccgagctg atcgtcagca accgcgccgg cggcccggtg cggctgcgcg
     5041 acgtggcgtc ggtcgaagac agctacgagt ccgtcaagac cttcgccagc ttcgacggcg
     5101 agcacgcgat ctcgctcgcg gtgttccgcc agcccgacgc caacacggtg aaggtcgtcg
     5161 atgcggtgcg cgccgcgatg ccggggtttc gctcgcagct gccgcactcg gtgcgcgtgt
     5221 cgctggtcaa cgaccgttcg gtgtcggtgc gcgccgcgct gcacgacgtg accctgacga
     5281 tgttcggcac gatcgtgctg gtggtgctgg tgatcttcct gttcctgcgc cgcttcatgg
     5341 cgacggtgat cccgacgctg tcgctgccgg tgtcgctgct cggcgcgatc gcgctgctcg
     5401 gcggcttcgg ctacacgctg gacaacatct cgctgctggg catcacgctg gcggtcgggc
     5461 tggtggtcga cgacgcgatc gtgatgctcg agaacatcat gcgccacgtc gagaacggcg
     5521 agccgccgtt ccaggccgcg ctgcgcggct cgcgcgaggt cgggttcacg atcctgtcga
     5581 tctcgacctc gctgatcgcg gtgttcatcc cgatcttctt catgcccggc gtgatcgggc
     5641 tgctgttcca cgagttcgcg gtcgtggtgt cgctggcgat catcgcgtcg gccttcgtct
     5701 cgctgacgct ggtgccgatg atggcggcgc gcttcctgag cgaccgggcg caccgcaagc
     5761 cgccgggtct ggtggtgcgc gcgttcgagc gcggcttcga cgcgctgctg gccggctaca
     5821 cgcgctcgct cgatgcggcg ctgcgccacc gcgggttcat cggctgcgtt gcggtgctca
     5881 cgctcgccgc gaccgcgtgg ctgttcgtga cgatcccgaa gggcttcttc ccggaagagg
     5941 acatcggcca gatccgcgtc acgaccgagg ccgccgagga cacctcgttc ccggcgatgg
     6001 tgcagctgca ggagcgcatc gccgacatct tccgcgccga cccgaacgtg gcgagcgtca
     6061 actcgttcaa cggcggcgac ggtgcgcaga acaccgggcg cctgttcctg aacctgaagc
     6121 cgcgcgccga acgcgcgccg atgaaggagg tcgtcgaagg cctgcgccgc aagctgcgcg
     6181 acgtgccggg catcaccgtg ttcatgcggc cgatccagaa cctgcagctc ggcgggcgcc
     6241 agagcaaggc gcagtaccag tacatcctgc agagcgtgag cgccgccgag ctgagcgact
     6301 gggccacccg gctgcaggac gcgatgcgcg tcgacccgct gtttcgcgac gtcaccagcg
     6361 attcgcagct caagggcctg caggcctcgc tgaagatcga ccgcgagcgc gccaacacgc
     6421 tgggcgtgtc catcgacgcg gtgcgcaacg cgctgtacag cgcgttcggc gagcgccagg
     6481 tctcgacgat ctacaccagc gtcgacagct accaggtgat catggaggtc gcgcccgagg
     6541 cgaagcagga cgagagcgcg ttcagcgcga tcttcgtgcg ctcgagcaac ggcacgctgg
     6601 tgccgctgtc ggcgttcacg gcggtcgagc gcacgatcgg gccgacctcg gtgaaccacg
     6661 ccggccagct gcaggcggtg acggtgtcgt tcaacctcgc gcccggcgcc gcgctcggcg
     6721 acgcgaccgc gaagctcgag cgctaccgcg accagatccg catgccggcg gctatcgtca
     6781 ccagctacgg cggcgacgcg gcggtgttca agagctcgca gggcagccag gtgatcctga
     6841 tcatcggcgc gctgctcgtg atctacgtgc tgctgggtgt gctgtacgag agctacgtgc
     6901 acccgctgac catcctcgcc gggctgccgt cggcggcggt cggcgcgctg ctgatgctgc
     6961 gcctgttcga catggacctg acgctgatcg cgaccatcgg catcctgctg ctgatcggca
     7021 tcgtcaagaa gaacgcgatc atgatgatcg acttcgcgct cgacgcgcag cgcacgcgcg
     7081 gcgcggcacc ggccgcggcg atccgcgagg cctgcgtgct gcgcttccgg ccgatcatga
     7141 tgacgacgct ggccgcgctg atgggcgcgc tgccgatcgc gctcggcctg ggcgccggcg
     7201 ccgagctgcg ccagccgctc gggctggcgg tggtgggcgg cctggtgttc tcgcaggcga
     7261 tcacgctcta catcacgccg gtgatctacc tcgcgctcga ccgcttcagc ggcaccgggc
     7321 cggtgacgac gccggagcag ccggccgctg tcaaaaaatc gggttgacga agtggggcac
     7381 ccggcggcgg atacgccgcc acccgccgag cgggtgctca ggccgcttgg gagcggcccg
     7441 gcgctggcct gagcgcgccg cggcgccgcc gcgtcagtgc agcgccgagc cgctcggcgc
     7501 cgtgttcatc acggtgaaca ccggctggcc gacgctgccc gcctcgacct cttcagcggt
     7561 ggcggcgcgc acgtcgcgca ccgtgaggtc caggcgcagc gcgatcccgg ccagcgggtg
     7621 gttgccgtcg agcacgacgt gggcggggta gacctcggtg acggtgtaga tggcgtcggc
     7681 cggcatgccc tgggtggtcg cgccgggcgg caggccgtcg aactgcatgc cgacctcgat
     7741 gacctcggaa aacaccgaac gcgcctcgaa gaacaccagc gccgggtcgt agtcgccgaa
     7801 ggcgtgctcg ggttccaggt gcagcgtggt cgtgaagccg gcggtctggc cggcgagcgc
     7861 ctcctcgacc ttggcgagca ggtcgtcgcc gccgtagaag aactcgaccg ggtcttgcaa
     7921 ttcgtcgatc aggctgccct gggtgtcctg caatttccag gtcaggctga cgacgcacgg
     7981 ggtggtaata ggcatcgttc gatgatcgca caatcgacca tggacatcac gcttgcga
//
LOCUS       DATMJB010000497         4646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_493772, whole genome shotgun sequence.
ACCESSION   DATMJB010000497 DATMJB010000000
VERSION     DATMJB010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493772"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1209
                     /locus_tag="VNU48_10675"
     CDS             <1..1209
                     /locus_tag="VNU48_10675"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWI11784.1"
                     /translation="GSFVETVTENDIEQVAAELAGARLPVLVGGAGIRNSAAAPLFRE
                     FAESLKCPVVFTHGGRGVLPDTHPQVLDYGLEPGLTVTRDADVVFVVGSSIGEKIGFG
                     GNPYFHGQEGFTNLFGERGRQRWIHLNQDVSAIGRNRDVDVALVGDMRRVLPRLTAAL
                     RRSPVNGTPQLPAWQQQRRERYRALFAEAPDMKPIHSGRMMVEIQKALPDDVVIINGG
                     GAVNLWTQYYQCHNIAGQLKSSKLGNLGTGVPYAMGAALALREAGNRVCVLGGDGGFG
                     FYPMELETAVRYQLPIVVIVGYDAGWSLEVPYYTYLTGMTFEVDHNFMRLDELARTMG
                     AHGEFCETTDQIAPAVRRAFASGKPALVQVVIDRATNAYKIPNLDQVSRFHADKQVYV
                     NRADTQAKED"
     gene            1224..2024
                     /locus_tag="VNU48_10680"
     CDS             1224..2024
                     /locus_tag="VNU48_10680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI11785.1"
                     /translation="MPELQAKVAIVTGAGSGIGRGIALGFLREGASVVAADIDAAKAE
                     ETAALARAEGWGDAVLVQRTDVTQEAEVEAVVRAAVARFGRLDCMVNNAGGPGAMEPL
                     LDMPTEGFDGTLALLLRSVFLGIKHAGRVMRAQGEGGIILSTASIAAQMCGASPSVYA
                     AAKAGVVQLSRMAAAELGAYRIRVNTVSPGAIYVPGFALGGVSKQQLTTVQSWPEAGM
                     PADVAAAMVFLAGDRCHFATGADFVVDGGLIAHGSRTLERLYGAQPNR"
     gene            2035..3258
                     /locus_tag="VNU48_10685"
     CDS             2035..3258
                     /locus_tag="VNU48_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWI11786.1"
                     /translation="MAGALSHVRVLDLSRILAGPWASQMLADLGADVIKVERPEVGDD
                     TRRWGPPFLKDRDGADTSDSAYYLCANRNKRSVTIDFTRPEGQRLIHDLAAKSDVVIE
                     NFKPGGLARFKLDYESLRRINPGIVYCSITGFGQTGPYAARAGYDFLMQGMGGLMSIT
                     GHADGEAGAGPVKVGVALTDILTGLHASTAILAALAHRDHGGTGQHIDLALLDVQVAC
                     LANQNMNYLHGGQVPRRLGNAHPNVVPYQDFPTADGDMILAIGNDGQFARLCQAIGRP
                     EWATDARFQTGPARVQHRQLLVPMLRLETVRRTTHDWIALLEASGVPCGPINNIAQVF
                     EDPQIVARGMKITMTHPLAGAIPLVASPIRMSATPPAYTLPPPRLGEHTRAVLGDVLA
                     LDEAACDRLAADGVI"
     gene            3292..4443
                     /locus_tag="VNU48_10690"
     CDS             3292..4443
                     /locus_tag="VNU48_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWI11787.1"
                     /translation="MIDTLSLAAIPAEDEALRGEVRAFIREKMGDVPAHVRARSWAGF
                     DAGFSRALAERGWVGITLPKALGGGGRSSFARFVLSEELLMAGAPVAAHWIADRQSAP
                     LIVKYGTEAQKHFYVPRVCRAEAFFCIGMSEPNSGSDLASVRTRATRTATGWQLNGQK
                     IWTTHASRSHYMIALVRTSGTPDDRNKGLSQLIVDLSLPGVVVRPIRDLAGDAHFSEV
                     FFDHVELGDDALIGTEGAGWEQVTAELAYERSGPERIYSSIVLLEPWLEWLRAQRDPS
                     MAQLAQAGAIASELAVLRSMSIAVTSRLAGGHSPVVEAALVKDLGTEYEQRLPAWLAD
                     TVHADPDLPPSHELERTLAYVTKVAPAFSLRGGTREILRGMIARGLGLR"
     gene            4455..>4646
                     /locus_tag="VNU48_10695"
     CDS             4455..>4646
                     /locus_tag="VNU48_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWI11788.1"
                     /translation="MQDDLLADSIEQLLAGLATPQAIRAIESGGSPAALWNAIEESGF
                     CDALVAEDEGGAGLGLDDVF"
BASE COUNT          737 a         1583 c         1611 g          715 t
ORIGIN      
        1 ggcagctttg tcgagaccgt gaccgagaac gatatcgagc aggtcgcggc cgagttggcg
       61 ggtgccaggt tgccggtgct tgtcgggggc gccggcatcc gcaacagcgc ggcggcgccg
      121 ctgttccgcg agttcgccga aagcctgaag tgcccggtcg tcttcaccca tggcgggcgc
      181 ggcgtgctgc cggacacgca tccccaggtg ctggactacg ggctggaacc gggcctgacg
      241 gtgacgcgcg acgccgatgt cgtgttcgtc gtcgggtcgt ccatcggcga gaagatcggc
      301 ttcggcggca acccctactt ccatggacag gagggcttca ccaacctgtt cggtgagcgt
      361 ggccggcagc gctggatcca tctcaaccag gacgtgagcg ccatcggccg caatcgcgat
      421 gtggacgtgg ccctggtagg ggacatgcgc cgtgtgctgc cgcgcctgac agcggccctg
      481 cgccgcagcc cggtcaacgg cacgccgcag ttgccagcct ggcagcagca gcggcgcgag
      541 cgataccggg ccctctttgc cgaggcgccg gacatgaagc ccatccactc cggccgcatg
      601 atggtggaga ttcagaaggc cctgccagac gatgtcgtga tcatcaacgg cggcggtgcc
      661 gtcaacctct ggacccagta ctaccagtgc cacaacatcg ccgggcagct caagtcgagc
      721 aagctcggca acctgggcac cggtgtgccg tacgcgatgg gcgcggcgct ggccctgcgc
      781 gaagccggca atcgggtctg tgtgctgggg ggggacgggg gattcggctt ctacccgatg
      841 gagttggaaa cggcggtgcg ctaccagttg cccattgtcg tgatcgtggg ctatgacgcc
      901 ggctggtcgc tggaggtgcc gtactacacg tacctcaccg gcatgacctt cgaggtggac
      961 cacaacttca tgcggctgga cgaattggcc cgaaccatgg gtgcgcatgg cgagttctgc
     1021 gaaaccaccg accagatcgc accggcggtg cggcgggcct ttgcgtccgg gaagccggcg
     1081 ctggtgcagg tggtcatcga ccgcgctacc aatgcctaca agattccaaa cctggaccag
     1141 gtgagccgct tccacgcgga caagcaggtc tacgtcaacc gtgcggatac ccaggccaaa
     1201 gaggactgaa ggagccgctg aacatgccgg aattacaggc gaaggtcgcc atcgtcaccg
     1261 gtgccggcag tggcatcggc cggggcatcg cattgggctt cttgcgcgag ggagccagtg
     1321 tggtggcggc cgatatcgat gcggccaagg ccgaggagac cgctgcgctg gctcgcgccg
     1381 aaggctgggg tgacgccgtg ctcgtgcagc gcaccgatgt cacgcaggag gccgaggtcg
     1441 aagcggtggt gcgtgcggca gtggcgcgat ttgggcgcct cgactgcatg gtcaacaacg
     1501 ccggcggtcc gggggccatg gagccgctgc tggacatgcc caccgagggt ttcgacggca
     1561 cgctcgcgct gctgctgcgc agcgtgttcc ttggcatcaa gcacgcgggc cgcgtcatgc
     1621 gcgcgcaggg cgagggcggg atcatcctct ccacggcatc gatcgcggcc cagatgtgtg
     1681 gcgcttcgcc cagcgtctac gctgccgcca aggccggcgt ggtgcagttg tcccgcatgg
     1741 cagcggcaga gctgggggcc taccgcatcc gtgtcaacac cgtgtcgccg ggagccatct
     1801 atgtgcccgg ctttgccctc ggcggggtat cgaagcagca gctcaccacc gtgcagtcgt
     1861 ggccggaggc tggcatgccg gccgatgtgg cggccgcgat ggttttcctg gcgggcgacc
     1921 gctgccactt tgccaccggg gccgacttcg tggtggacgg cggcctcatc gcacacggct
     1981 cgcgcaccct ggagcgtctg tacggcgccc agccgaaccg gtagccacgc agccatggcc
     2041 ggcgcgcttt cgcatgtccg ggtgctcgat ctgtcgcgca tcctcgccgg tccctgggcg
     2101 agtcagatgc tcgcggacct gggcgccgac gtcatcaagg tcgagcggcc cgaggtcggc
     2161 gatgacacgc gcaggtgggg gccgccgttc ctgaaggacc gcgacggggc ggacaccagc
     2221 gactcggcct actacctgtg cgcgaaccgc aacaagcgct cggtgacgat tgacttcaca
     2281 cgccccgagg gacagcgcct gatccacgat ctggcggcga agagcgacgt cgtgatcgaa
     2341 aacttcaagc caggcggcct cgcgcgcttc aagctcgact acgagtcgct gcggcggatc
     2401 aaccctggca tcgtctattg ctcgatcacc ggcttcggcc aaaccggtcc ctacgcggca
     2461 cgcgccggct acgacttcct catgcagggc atgggcgggc tgatgagcat caccggccac
     2521 gccgacggag aagcgggcgc gggcccggtc aaggtgggcg tcgcgttgac cgacatcctc
     2581 accggcctgc acgcgtcgac cgcgatcctc gcggcgctcg cgcatcgcga ccacggcggc
     2641 accggccagc acatcgacct cgcgctgctc gacgttcagg tggcgtgcct ggccaaccag
     2701 aacatgaact acctgcacgg cggccaggtg ccacgccgac tcggcaacgc ccatccgaac
     2761 gtcgttccct accaggactt cccgaccgcc gacggcgaca tgattctcgc catcggcaac
     2821 gacggccagt tcgccaggct gtgccaggcg atcggccggc cggagtgggc gaccgacgcg
     2881 cgctttcaga ccgggcccgc gcgggtgcag caccgccaac tcctggtgcc gatgctgcgc
     2941 ctcgagaccg tgcgccggac gacccacgac tggatcgcgc tgctcgaggc gtccggcgtg
     3001 ccgtgcggac cgatcaacaa catcgcccag gtcttcgagg atccgcagat cgtcgcgcgc
     3061 ggcatgaaga tcacgatgac gcatccgctg gccggcgcga tcccgctggt ggcgagcccg
     3121 atccggatgt cggcgacgcc cccggcctat acgctgccgc cgccgcgtct gggcgagcac
     3181 acgcgcgccg tcctcggcga tgtgctcgcg ctcgacgaag cggcgtgcga tcggctggcg
     3241 gccgacggtg tcatctgagc acgcgtcgcg ccacgactcg acgcaaacgc catgatcgac
     3301 acgctgtcgc tcgccgccat tcccgccgag gacgaagcgc ttcgcggcga agtccgggcg
     3361 ttcatccgcg agaagatggg cgacgtcccc gcccacgtgc gggcgcgctc gtgggcaggg
     3421 ttcgacgccg ggttcagccg agcgctcgcc gagcgcggct gggtcggcat cacgctgccg
     3481 aaagcgctag gcggcggcgg ccgcagcagc ttcgcgcgct tcgtgctgtc cgaggaactg
     3541 ctgatggcgg gcgcaccggt ggcggcgcac tggatcgccg atcgccagag cgcgccgctg
     3601 atcgtcaagt acggcaccga agcgcagaaa catttctacg ttccgcgcgt gtgccgcgcc
     3661 gaggccttct tctgtatcgg catgagcgag cccaattccg gctccgacct ggcgagcgtg
     3721 cgcacccgcg ccacccgcac cgcgaccggc tggcaactga acggccagaa gatctggacc
     3781 actcacgcat cgcgctcgca ctacatgatc gcgctcgttc gcacgtcggg cacgccggac
     3841 gaccgcaaca aggggctgtc gcagctgatc gtcgacctgt cgctgccagg cgtggtggtg
     3901 cggccgatcc gcgacctggc gggcgacgca cacttctccg aggtcttctt cgaccacgtc
     3961 gagctgggcg acgacgcgct gatcggcacc gagggcgccg gctgggaaca ggtgaccgcg
     4021 gagctcgcgt acgagcgcag cggaccggag cgaatctatt cgagcatcgt gctgctcgag
     4081 ccctggctcg aatggctgcg cgcgcagcgc gacccctcga tggcccagct cgcccaggcc
     4141 ggcgccattg catcggaact cgccgtgctg cgcagcatgt cgatcgccgt cacgtcgcgg
     4201 cttgcgggcg gacacagccc cgtcgtcgaa gccgcgctgg tgaaggacct gggtaccgaa
     4261 tacgagcagc ggctgcctgc ctggctggcc gacaccgttc atgccgaccc cgatctcccg
     4321 ccttcgcacg agctcgagcg aaccctcgcc tacgtgacca aggtcgcgcc ggccttttcg
     4381 ctgcgcggcg gcacgcgcga gatcctgcgc gggatgatcg cgcgcggcct cggccttcgc
     4441 tgaaggacac gacgatgcag gacgatctgc ttgccgattc gatcgagcaa ctgctcgccg
     4501 gcctggcgac gccgcaggcg atccgcgcca tcgaaagcgg cggctcgccc gcggcgctgt
     4561 ggaacgcgat cgaggaatcg ggcttttgcg acgcgctcgt ggccgaggac gaaggcggtg
     4621 ccgggctcgg cctcgacgat gtcttt
//
LOCUS       DATMJB010000498         3406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_727721, whole genome shotgun sequence.
ACCESSION   DATMJB010000498 DATMJB010000000
VERSION     DATMJB010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727721"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /gene="hemF"
                     /locus_tag="VNU48_10700"
     CDS             <1..615
                     /gene="hemF"
                     /locus_tag="VNU48_10700"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HWI11789.1"
                     /translation="VPTVHMNVRMLSAQPAGGAPVRWFGGGMDLTPYYGFEEDAAHFH
                     RVNRDALAPFGDDKYPRFKRWCDEYFFLKHRSEPRGIGGVFFDDFAELGFERSFAMLR
                     AVGDAFLPAYLPILQRRRAMPFGARERDFQTYRRGRYVEFNLVFDRGTLFGLQSGGRT
                     ESILMSMPPVVTWRYDWRPEAGSPEARLYSDFLRPRDWADWTAA"
     gene            612..1217
                     /gene="nadD"
                     /locus_tag="VNU48_10705"
     CDS             612..1217
                     /gene="nadD"
                     /locus_tag="VNU48_10705"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWI11790.1"
                     /translation="MSAAARVGVFGGSFDPVHNAHLALARVALSELHLDELLWVPAGQ
                     PWQKQRPMTAAAHREAMVRLAIAGEPRFVLSRIELERRGPSYSVDTVRALHAQRPGVD
                     WFLVIGQDQYARFHTWHGWQELLGLVTLAIANRPDAPVTGDPQLLSVPHAAVALPMMD
                     VSSTDIRERIAHGQGIADLVPAPVASYIARHHLYQDPPPRS"
     gene            1218..1880
                     /gene="rsfS"
                     /locus_tag="VNU48_10710"
     CDS             1218..1880
                     /gene="rsfS"
                     /locus_tag="VNU48_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWI11791.1"
                     /translation="MDIRKLQRVIVDGLEDVKAQNIVVFNTEHLSALFERVIIASGTS
                     NRQTKSLAGSVRDAVKTAGYPVPRTEGESNGEWIIVDCGAAVVHVMQPTIRDYYHLEE
                     IWGDKPVNMKLATGGAKLVKAAEPDAPVRKAAAKKAAAKKAAPRAPAKAPPRKPVAAK
                     TTAAKVVAKKAPARKAPPGKVVTRVVGVKSSAAKREPAKAAPAKRPAAKKAPARKTTS
                     RA"
     gene            1877..2344
                     /gene="rlmH"
                     /locus_tag="VNU48_10715"
     CDS             1877..2344
                     /gene="rlmH"
                     /locus_tag="VNU48_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HWI11792.1"
                     /translation="MKLLVLAVGQRPPAWAQTAYTEFEKRFPPEMRLELHAVKAEARG
                     HKSAEQLMAAEAARLQAALPRGVRRVVLDERGSRVTTVQLAERMRAWLHDGRDVAFLI
                     GGPDGLDATLKASADETLRLSELTLPHAFVRVLLAEALYRAWTVTVNHPYHRE"
     gene            2337..2939
                     /locus_tag="VNU48_10720"
     CDS             2337..2939
                     /locus_tag="VNU48_10720"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWI11793.1"
                     /translation="MNDFIYLASQSPRRRQLLAQIGVRVELLLPRADEDAEALEAERP
                     GELPATYVERVTRAKLKAARARLRLSGAPDATILCADTTVALGRRILGKPRDADDAIA
                     TLTALSGRTHRVITAVAVAAGRRERIAVSCSHVQVAALPAAAIAAYVAGGEPFGKAGA
                     YAIQSGFAAWIERIEGSYSGIMGLPLFETAQLLRGFGIGV"
     gene            2988..>3406
                     /locus_tag="VNU48_10725"
     CDS             2988..>3406
                     /locus_tag="VNU48_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HWI11794.1"
                     /translation="MQDILINWTPQETRVAVVENGAVQELHVERALERGLVGNIYAGK
                     VARVLPGMQSAFIDIGLERAAFLHVADVQVNGVVPRSSFGAGRADGAGNGPPPPPIER
                     LVFEGQTLTVQVIKDPIGTKGARLSTQISIAGRLLVFL"
BASE COUNT          517 a         1182 c         1235 g          472 t
ORIGIN      
        1 gtgccgaccg tgcacatgaa cgtgcgcatg ctgtcggcgc agcccgccgg cggcgcgccg
       61 gtgcgctggt tcggcggcgg catggacctg acgccgtact acggcttcga ggaggacgcg
      121 gcgcatttcc atcgcgtcaa ccgcgacgcg ctcgcgccct tcggtgacga caagtaccca
      181 cgcttcaagc gctggtgcga cgagtacttc ttcctcaagc accgcagcga gccgcgcggc
      241 atcggcggcg tgttcttcga cgactttgcc gaactcggct tcgagcgcag cttcgcgatg
      301 ctgcgtgcgg tcggcgatgc gttcctgccc gcctatctgc cgatcctgca gcgccgccgg
      361 gccatgccct ttggcgcgcg cgagcgcgac ttccagacct accggcgcgg ccgctatgtc
      421 gagttcaacc tggtgttcga tcgcggcacc ctgttcgggc tgcagtcggg cgggcgcacc
      481 gagagcatcc tgatgtcgat gccgccggtc gtcacctggc gctacgactg gcggcccgaa
      541 gcgggttcgc ccgaggcgcg gctgtacagc gacttcctgc gcccgcgcga ctgggccgat
      601 tggacggccg cttgagtgcc gctgcgcgtg ttggtgtctt cgggggcagt ttcgacccgg
      661 tgcacaacgc gcacctcgcg ctcgcgcgcg tcgcgctctc cgagttgcac ctcgacgaac
      721 tgctgtgggt gccggccggc cagccctggc agaagcagcg cccgatgacc gccgccgcgc
      781 atcgtgaggc gatggtgcgt ctggcgatcg ccggcgagcc gcgcttcgtg ctgtcgcgca
      841 tcgagctcga gcgccgcggc ccgagctaca gcgtcgacac ggtgcgtgcg ctgcacgcgc
      901 agcggcccgg ggtcgattgg ttcctcgtga tcgggcagga ccagtacgcg cgctttcaca
      961 cctggcacgg ctggcaggag ctgctcggcc tggtcacgct ggcgatcgcg aatcgccccg
     1021 acgcgcccgt caccggcgac ccgcagctgc tgagcgtgcc gcacgccgcg gtggcgctgc
     1081 cgatgatgga cgtgtcgtcg accgacatcc gcgaacgcat cgcgcacggg cagggcatcg
     1141 ccgacctggt gccggccccg gtggcgagct atattgcccg ccatcacctt tatcaagatc
     1201 ccccacccag gagctgaatg gacattcgta aactgcaacg cgtcatcgtc gatggactgg
     1261 aagacgtcaa ggcccagaac atcgtggtgt tcaacaccga gcacctgtcg gccctgttcg
     1321 agcgcgtgat catcgcctcg ggcacgagca accggcagac caagtcgctg gccgggagcg
     1381 ttcgcgatgc agtcaagacc gcgggctacc cggtgccacg caccgagggc gagagcaacg
     1441 gcgaatggat catcgtcgac tgcggcgccg ccgtcgtgca cgtgatgcag ccgacgatcc
     1501 gcgactacta ccacctcgag gagatctggg gcgacaagcc ggtcaacatg aagctcgcga
     1561 ccggtggcgc gaagctggtg aaggcggccg agcccgatgc gccggtcagg aaggcggccg
     1621 cgaagaaggc cgcggcgaag aaggcggcgc cgcgagcgcc cgcgaaggcg ccgccgcgaa
     1681 agccggtggc ggccaagacc acggccgcga aggtcgtggc gaagaaggca ccggccagga
     1741 aggcgccgcc cggcaaggtc gtcacgcgcg tcgtcggcgt caagtcgagc gcggccaagc
     1801 gcgagccggc caaggcggcg ccggcgaagc ggccggcagc gaagaaggca cccgcgcgga
     1861 agacgaccag ccgggcatga agctgctggt gctcgcggtc ggccagcggc cgccggcctg
     1921 ggcacagacc gcctacaccg agttcgagaa gcgcttcccg cccgagatgc ggctggagct
     1981 gcatgcagtg aaagccgagg cgcgcggcca caagagcgcc gagcagctga tggccgccga
     2041 ggctgcgcgg ctgcaggccg cactgccgcg cggcgtgcgt cgcgtcgtgc tcgacgagcg
     2101 cggcagccgc gtcacgacgg tgcagctcgc cgagcgcatg cgcgcgtggc tgcacgacgg
     2161 gcgcgatgtc gcattcctga tcggcggccc cgacggcctg gacgcgacgc tgaaggcgag
     2221 cgccgacgag acgctgcgcc tgtccgaact gaccttgccg catgccttcg tgcgcgtgct
     2281 gctcgccgag gcgctgtacc gtgcgtggac cgtgaccgtg aaccacccgt atcaccgtga
     2341 atgatttcat ctacctcgcg tcgcagagcc cgcgccggcg ccagctgctg gcgcagatcg
     2401 gcgtgcgcgt cgagctgctg ctgccgcgcg ccgacgagga cgccgaggcg ctcgaggccg
     2461 agcgcccggg cgagctgccg gccacgtacg tcgagcgcgt gacgcgcgcg aagctgaagg
     2521 cggcgcgcgc gcggctgcgg ctgagcggcg cgcccgacgc gaccatcctg tgcgccgaca
     2581 ccaccgtcgc gctcgggcgg cgcatcctgg gcaagccgcg cgatgccgac gatgcgatcg
     2641 cgacgctgac cgcgttgtcg gggcgcacgc accgcgtgat caccgcggtg gccgtcgccg
     2701 cgggccggcg cgagcggatc gcggtgagct gctcgcacgt gcaggtcgcg gcgttgccgg
     2761 cggcggcgat cgcagcctat gtcgccggcg gcgaaccctt cggcaaggcc ggcgcgtacg
     2821 cgatccagag cggcttcgcg gcgtggatcg agcgcatcga agggagttac tcgggcatca
     2881 tgggcctgcc gctgttcgag accgcgcagc ttctgcgagg cttcggcatc ggcgtctgag
     2941 ttcccgcgcc gggccatgcg agcacaacga gaaagagacg aacgcccatg caagacatcc
     3001 tgatcaactg gacgccgcag gagacccgcg tcgcggtggt cgagaacggc gccgtccagg
     3061 agctgcacgt cgagcgcgcg ctcgaacgcg gcctggtcgg caacatctac gccggcaagg
     3121 tggcgcgcgt gctgccgggc atgcagtcgg cgttcatcga catcggcctg gagcgcgcgg
     3181 cgttcctcca tgtcgccgat gtgcaggtca acggcgtggt gccgcgcagc agcttcggcg
     3241 ccgggcgcgc cgacggcgcc ggcaacgggc cgccgccgcc gccgatcgag cgcctggtgt
     3301 tcgagggcca gacgctgacg gtgcaggtca tcaaggaccc gatcggcacc aagggcgcgc
     3361 ggctgtcgac gcagatcagc atcgccgggc ggctgctcgt gttcct
//
LOCUS       DATMJB010000499         6974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_771747, whole genome shotgun sequence.
ACCESSION   DATMJB010000499 DATMJB010000000
VERSION     DATMJB010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771747"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VNU48_10730"
     CDS             complement(<1..546)
                     /locus_tag="VNU48_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWI11795.1"
                     /translation="MNLSKFFIDRPIFAGVLSLLIFLGGLIAVRGLPISEYPDVVPPS
                     IVVRANYPGANPKVIAETVATPIEEQINGVEDMLYMSSQATTDGLMTLTVTFKLGTDP
                     DKAQQLVQNRVARAEARLPEEVRRLGITTVKSSVDLTMVVHLLSPTGRYDMTYLRNYA
                     VLNVKDRLARVEGVGDVQLFGS"
     gene            complement(564..1787)
                     /locus_tag="VNU48_10735"
     CDS             complement(564..1787)
                     /locus_tag="VNU48_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWI11796.1"
                     /translation="MSNRRLPLPARRRLWVAGTVLTALTAIATAVFSLRSGSADASPA
                     AAPPATAVSVATVVASDVATWDEFSGRLEAVERVDIRSRVAGTVQAVHFREGALVKKD
                     ELLLTIDPAPYAAEVDRAEAQVAAAQARLSYTRSEFERAQRLLDERAIAQREADERIN
                     AQREADANLRAAQAALQTARLSLGYTQVRAPVAGRVGRLEITVGNLVAAGPGAPVLTT
                     LVSVSPIYASFDADEQVITRALRDLSSGSSARAQLEKIPVQMGTAATADTPFTGHLQL
                     VDNQVDAKSGTVRVRAVFDNADGSLIPGQFAKLRMGQIKSTAALLVNERAVGTDQNKK
                     YVMVVGNDNKAAYREVTLGAHVQGLRIVTAGLKPDERVIVNGLQRVRPGALVAPQPVA
                     MDIKAEAARDATPRS"
     gene            2001..2660
                     /locus_tag="VNU48_10740"
     CDS             2001..2660
                     /locus_tag="VNU48_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWI11797.1"
                     /translation="MAAPAPPKPRGRPLSFDRDAALDRAMHVFWEHGYEAASISDLTA
                     AMGITPPSLYTAFGDKERLFLEAIERYGKGPGGFGQRALDEEPTARGAIQRLLEEAAA
                     ELTEECHPLGCMMVMATTNCSVAAEHVQTALAKRRALGVSNIQARIQRGIADGELPPD
                     TDAGALANFYATVYQGMSMQAKDGAPRASLLASVAMAMRSWPPAPPARRIAKKRVAAS
                     T"
     gene            complement(2691..4232)
                     /gene="glpD"
                     /locus_tag="VNU48_10745"
     CDS             complement(2691..4232)
                     /gene="glpD"
                     /locus_tag="VNU48_10745"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HWI11798.1"
                     /translation="MLVVGGGINGTGIARDLAGRGLSVVLCEKDDLAQHTSSSSTKLI
                     HGGLRYLEHGEFGLVRKALAEREVLLESAPHIMWPLRFVMPHDPGMRPVWMIRAGLFL
                     YDHLARRELLPASATVDLRRHPAGRPLKPSFTKGFVYSDGWVDDARLVVLNAIDAAER
                     GAVVLTRWQCVAARRGPASWQLRLESARGERRELTARALVNAAGPWAARFLSECARLP
                     RAKSLRLVKGSHIVVPKMFDHDHAYIFQNPDKRVIFAIPYEHDFTLIGTTDVEHRGAI
                     GEARIDAAETAYLCEQASRYFAKPVTPQEVVWSYAGVRPLLDDESGDPSAVTRDYLLE
                     LDTEGATLLTVWGGKITTFRKLAEEAADLLAARLGTTRGAWTRGAFLPGGELAAWVGK
                     ARRARADVVGDFARFVQAVTQRYPQVPPALCRRLARAYGTRVEQLLASGALGAEVAPG
                     LFEAELGHLHDHEWARSADDVLWRRSKLGLHFDAAQRDAVAAWCDRHWPDHAGSASSS
                     STTPR"
     gene            4484..6037
                     /gene="glpK"
                     /locus_tag="VNU48_10750"
     CDS             4484..6037
                     /gene="glpK"
                     /locus_tag="VNU48_10750"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HWI11799.1"
                     /translation="MTPNRTHLLALDQGTSSSRSIVFDAGGRIVAIAQREFRQIYPQP
                     GWVEHDPKEIWAGQLATAREALAKAGLTADAIAAIGITNQRETTVLWDRRSGEPLYNA
                     IVWQDRRTEPTCAALRERGLEPTFRAKTGLILDAYFSGTKLKWLLDHVPGARAAALRG
                     ELAFGTIDAWLMWMLTGGADDSTRQHALHATDVSNASRTLLLNVHDNAWDDELLGLLE
                     IPRELLPSVHPSSHVYGHTQTDLLGAPIPIGGVAGDQQSALFGQACFKAGLAKNTYGT
                     GCFLLMHTGERFLSSTNGLITTAAAQLGTTSAAQPSAAPEFALEGSVFIGGAVVQWLR
                     DGLRAIPASGEVQALAESVPDSGGVMFVPAFTGLGAPYWKPDARGAIVGLSRGSTVAH
                     IARAALESIAFQSAALLAAMSRDAVAAGGAPVAELRVDGGACVNDLLMQFQADLLGIP
                     VVRPQVIETTALGAAYLAGLACGLYRNLDELAAQWRVERTFHPTLPRARAAELMANWE
                     HAVRQVVAS"
     gene            complement(6150..6707)
                     /locus_tag="VNU48_10755"
     CDS             complement(6150..6707)
                     /locus_tag="VNU48_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11800.1"
                     /translation="MLNDLKTQFSHSMQTMRRLLQQPIAADESARSPRSQAREERRQR
                     VRRMRRDLYQLLEQHPASRELMRHLDLVERTLRVQGLTAVEALPARVIAKALVQLERL
                     VWDWSPVGLAELRSRLAVIVRNQPIEVRAPAAPLANPPAPTPIARSIPQELVEVSEVD
                     HATFEEMERSWHGRVPEAPSAAQAA"
BASE COUNT         1015 a         2459 c         2465 g         1035 t
ORIGIN      
        1 cgagccgaac agctgcacgt cgccgacgcc ttcgacgcgg gcgagccggt ccttgacgtt
       61 gagcaccgcg tagttgcgca ggtaggtcat gtcatagcgg ccggtgggcg acagcaggtg
      121 cacgaccatc gtcaggtcga ccgagctctt cacggtcgtg atgccgagcc gccgcacctc
      181 ctcgggcagc cgcgcctcgg cgcgcgcgac gcggttctgc accagttgct gcgccttgtc
      241 ggggtcggtg ccgagcttga aggtgacggt cagcgtcatc agaccgtcgg tcgtcgcctg
      301 gctgctcatg tagagcatgt cttcgacgcc gttgatctgc tcctcgatcg gcgtcgcgac
      361 cgtctcggca atcaccttcg ggttcgcgcc ggggtagttc gcacgcacca cgatcgacgg
      421 cggcacgaca tcggggtatt ccgagatcgg cagcccgcgc accgcgatca ggccaccgag
      481 gaagatcagc agcgacagca cgccggcgaa gatcggccgg tcgatgaaga atttcgagag
      541 gttcatgaga gagcttcctg cgcttacgag cgcggggtcg cgtcgcgcgc ggcctcggcc
      601 ttgatgtcca tcgccaccgg ctgcggcgcc accagcgcgc ccgggcgcac gcgctgcaga
      661 ccgttgacga tcacgcgctc gtcgggcttc aggcccgcgg tcacgatgcg caggccctgg
      721 acgtgcgcgc ccagcgtcac ctcgcggtac gccgccttgt tgtcgttgcc cacgaccatc
      781 acgtacttct tgttctggtc ggtgccgacc gcgcgctcgt tgacgagcag cgcggccgtg
      841 gatttgatct ggcccatccg cagcttcgcg aactggcccg ggatcaggct gccatcggcg
      901 ttgtcgaaca ccgcgcgcac gcgcaccgtg ccgctcttcg cgtcgacctg gttgtcgacg
      961 agctgcaggt ggccggtgaa cggggtgtcg gcggtcgccg cggtgcccat ctgcaccggg
     1021 atcttttcga gctgcgcgcg tgcgctcgac ccgctcgaca gatcgcgcag cgcgcgggtg
     1081 atgacctgct cgtcggcatc gaagctggcg tagatcgggc tgaccgacac cagggtcgtc
     1141 agcaccggcg cgccgggccc ggcggcgacg aggttgccca cggtgatctc gagccgcccg
     1201 acgcggccgg cgaccggcgc gcgcacctgc gtgtagccca ggctcaggcg tgcggtctgc
     1261 aacgcggcct gcgcggcgcg caggttcgcg tcggcctcgc gttgcgcgtt gatgcgctcg
     1321 tcggcctcgc gctgtgcgat cgcgcgctcg tcgagcaggc gctgcgcgcg ctcgaactcg
     1381 ctgcgtgtgt acgacaggcg cgcctgcgcc gcggcgacct gcgcttcggc gcggtcgact
     1441 tcggccgcat agggcgccgg gtcgatcgtc agcagcagct cgtccttctt gaccagcgcc
     1501 ccttcgcgga agtgcaccgc ctgcacggtg ccggccacgc gcgaacggat gtcgacgcgt
     1561 tcgaccgctt cgagccggcc cgagaattcg tcccaggtcg cgacgtcgct ggcgacgacg
     1621 gtcgcgaccg acacggcggt ggctggcggc gcggccgcag gcgatgcatc ggcgctgccc
     1681 gagcgcaggc tgaacaccgc ggtggcgatc gccgtgagcg cagtcagcac ggtgccggcg
     1741 acccagaggc ggcggcgggc ggggagcggg aggcggcggt tcgacatgat gattcccttc
     1801 gtcggttcgg tcgtcggcaa ggctccgccc agcccgccga ggatcgattt cggcggcgag
     1861 aattgcggaa ctggaggcct caggccgggg tcagatattt cgtagtgaac gctacggcat
     1921 gtggcacaat gtagccatcg cacaaggcct cgtcaagaga tttattagcg atcgttatga
     1981 aaaagacaat ggagacaacg gtggccgcgc ccgcgccacc gaaaccgcgc ggccggccgc
     2041 tgtcgttcga ccgcgacgcc gcgctcgacc gcgcgatgca cgtgttctgg gagcacggct
     2101 acgaggccgc gtcgatcagc gacctgaccg ccgcgatggg catcacgccg cccagtctgt
     2161 acaccgcgtt cggcgacaag gagcgcctgt tcctcgaggc gatcgagcgc tacggcaagg
     2221 gcccgggcgg cttcggccag cgcgcgctcg acgaggagcc gaccgcgcgc ggcgcgatcc
     2281 agcgcctgct cgaagaggcc gccgccgagc tgaccgagga atgccacccg ctgggctgca
     2341 tgatggtgat ggcgacgacc aattgctcgg tggctgccga gcacgtgcag actgcgctcg
     2401 cgaagcgccg cgcgctcggt gtttcgaaca tccaggcccg catccagcgc ggtatcgccg
     2461 acggcgaatt gccacccgac accgacgccg gtgccctcgc gaacttctac gccaccgtct
     2521 accagggcat gtcgatgcag gccaaggacg gcgccccgcg cgcgagcctg ctggccagcg
     2581 tcgcgatggc gatgcgctcg tggccgccgg cgccgcccgc acggcgtatc gcgaagaagc
     2641 gggtcgccgc gtccacgtag ttcagagggc cggcaggacc gtcacggtcg tcaccgaggg
     2701 gtcgtcgatg aactgctggc agacccggcg tggtcgggcc agtgccgatc gcaccaggcg
     2761 gcgacggcgt cgcgctgtgc ggcgtcgaag tgcagcccga gcttgctgcg acgccacagc
     2821 acatcgtcgg cgctgcgcgc ccattcgtgg tcgtgcagat gaccgagttc ggcttcgaac
     2881 aggcccggcg cgacctcggc gccgagcgcc ccgctcgcca gcaactgctc gacacgcgta
     2941 ccgtaggcgc gcgccagccg ccggcacagg gccggcggca cctgcgggta gcgctgcgtc
     3001 acggcctgca caaagcgggc aaagtcgccg accacatcgg cgcgcgcgcg ccgcgccttg
     3061 ccgacccagg cggcgagctc gccgcccggc aggaacgcgc cgcgcgtcca ggcgccgcgc
     3121 gtggtgccca gccgcgccgc gagcagatcg gcggcctctt cggcgagctt gcggaaggtc
     3181 gtgatcttgc cgccccagac ggtcagcagc gtcgcgccct cggtgtcgag ctcgagcagg
     3241 tagtcgcgcg tcaccgccga cgggtcgccc gattcatcgt cgagcagcgg gcgcacgccg
     3301 gcgtagctcc agaccacctc ctgcggcgtc accggcttgg cgaagtagcg gctcgcctgt
     3361 tcgcacaggt aggcggtctc ggccgcgtcg atgcgggctt cgccgatcgc gccgcggtgc
     3421 tccacgtcgg tcgtgccgat cagcgtgaag tcgtgctcgt aggggatcgc gaagatgacg
     3481 cgcttgtcgg ggttctggaa gatgtaggcg tggtcgtggt cgaacatctt cggcacgacg
     3541 atgtggctgc ccttgaccag ccgcagcgac ttcgcgcgcg gcagccgtgc gcactcgctg
     3601 agaaaccgcg cggcccaggg cccggcggcg ttgacgagcg cgcgcgccgt cagctcgcgc
     3661 cgctcgccgc gcgcgctctc cagacgcagc tgccacgatg ccggcccgcg tcgtgcggcc
     3721 acgcactgcc agcgcgtcag cacgaccgcg ccgcgttcgg cggcgtcgat cgcgttcagc
     3781 accaccaggc gcgcgtcgtc gacccagccg tcggaataga caaagccctt cgtgaacgac
     3841 ggcttgagcg gccgccccgc ggggtggcgg cgcaggtcga cagtcgccga cgcgggcaac
     3901 agctcgcggc gcgcgaggtg gtcgtacagg aacagcccgg cgcggatcat ccacaccggc
     3961 cgcatccccg gatcgtgcgg catcacgaag cgcagcggcc acatgatgtg cggcgcgctc
     4021 tcgagcagca cctcgcgttc ggccagcgcc ttgcgcacca ggccgaattc gccgtgctcg
     4081 aggtagcgca ggccaccgtg gatcagcttg gtgctgctcg acgacgtgtg ctgcgccagg
     4141 tcgtccttct cgcacagcac gacgctgagg ccgcgcccgg ccaggtcgcg cgcgatgccc
     4201 gtgccgttga tgccgccccc gaccacgagc acgtcgcacg caggcgggca cgcatcgacc
     4261 gcggccgagc gcggcggctc gacccggggg tggggcgggt tcaatgaatt cctcggtttg
     4321 atcacaaacg gggtcgatgc gcgcgttcgt ttgtgttcgt tgttggtctt ctgttttcca
     4381 ttcttaccca gagaattcgc aaagcgcgag agagatgatc ccgatggccg gcatgacgcg
     4441 ccccgctacg ctggctcacc gccccgcaaa cgcccgcctt gccatgacgc cgaaccgcac
     4501 ccatctgctc gcgctcgacc agggcacctc cagctcgcgc agcatcgtgt tcgacgcggg
     4561 cggccggatc gtcgcgatcg cgcagcgcga attccgccag atctacccgc agccgggctg
     4621 ggtcgagcac gacccgaaag agatctgggc cggccagctc gccaccgcgc gcgaggcgct
     4681 cgccaaggcc gggctgacag ccgacgcgat cgcggcgatc ggcatcacga accagcgcga
     4741 gaccaccgtg ctgtgggacc ggcgcagcgg cgagccgctg tacaacgcga tcgtctggca
     4801 ggaccgccgc accgagccga cctgcgcggc gctgcgcgag cgcggcctgg agccgacgtt
     4861 tcgcgccaag accgggctga ttctcgatgc ctacttctcg ggcaccaagc tgaagtggct
     4921 gctcgaccac gtgcccgggg cacgcgctgc ggcgctgcgc ggcgaactcg cgttcggcac
     4981 gatcgatgcg tggttgatgt ggatgctgac cggcggcgcg gacgacagca cgcgccagca
     5041 cgcgctgcac gcgactgacg tgagcaacgc ctcgcgcacg ctgctgctga acgtgcacga
     5101 caacgcctgg gacgacgaat tgctgggcct gctcgagata ccgcgcgagc tgctgcccag
     5161 cgtgcacccg tcgagccatg tctacggcca cacgcagacg gacttgctcg gcgcgccgat
     5221 tccgatcggc ggcgtcgcgg gcgaccagca aagtgcgctg ttcggccagg cctgcttcaa
     5281 ggccgggctg gcgaagaaca cctacggcac cggctgcttc ctgctgatgc acaccggcga
     5341 acgcttcctc tcctcgacca acggcctgat cacgaccgct gctgcgcagc tcggcacgac
     5401 gagtgccgca cagccgagcg cggctcccga gttcgcactc gaaggcagcg tcttcatcgg
     5461 cggcgccgtc gtgcaatggc tgcgcgacgg cctgcgtgct attcccgcca gcggcgaggt
     5521 gcaggcgctg gccgagagcg tgcccgacag cggcggcgtg atgttcgtgc cggccttcac
     5581 cggcctcggc gcgccttact ggaagccgga cgcgcgcggt gcgatcgtcg gattgagccg
     5641 cggcagcacc gtggcgcaca tcgcgcgtgc cgcgctcgag agcatcgcgt tccagagcgc
     5701 cgcgctgctg gcggcgatga gccgcgacgc ggtcgccgcc ggcggcgcgc cggtggccga
     5761 actgcgcgtc gacggcggcg cgtgcgtcaa cgatctgctg atgcagttcc aggccgatct
     5821 gctgggcatc ccggtcgtgc gcccgcaggt gatcgagacc accgcgctcg gcgcggccta
     5881 tctcgcgggc ctggcgtgcg ggctgtaccg caacctcgac gaactggctg cgcagtggcg
     5941 cgtcgagcga acctttcatc cgacgctgcc gcgcgcgcgc gcggccgagc tgatggcgaa
     6001 ctgggagcac gcggtgcgcc aggtggtggc gagctgaagg ctgttgacat cacgttgtca
     6061 gcaggccggg tccagcgaga ttccggtgcg cgacctcgag accgagatgg ccgagcgcga
     6121 cagcagcacc gacgcctgaa cgcaccgcgt caggcggcct gcgcggcgct cggtgcctcg
     6181 ggcacgcggc cgtgccagct gcgctccatc tcttcgaagg tcgcgtggtc cacctcactc
     6241 acctcgacca gctcctgggg gatgctgcgc gcgatcggtg tcggtgccgg cgggttggcc
     6301 agtggagcgg ccggcgctcg gacctcgatc ggctgattcc tgacgatcac cgccaggcgc
     6361 gagcgcagtt cggccagacc gaccggcgac cagtcccaga ccagccgctc gagctgcacc
     6421 agcgccttcg cgatgacgcg cgccggcagc gcttcgaccg cggtgaggcc ctgcacgcgc
     6481 agcgtgcgct cgaccaggtc gaggtggcgc atcaactcgc gcgaggccgg gtgttgttcg
     6541 agcagctggt acaggtcgcg gcgcatccgg cgcacgcgct ggcggcgctc ctcgcgcgcc
     6601 tgcgaacgcg ggctgcgcgc cgactcgtcc gcggcgatcg gctgctgcag caagcgccgc
     6661 atcgtctgca tcgaatggct gaactgcgtt ttcaggtcgt tgagcatggt gttggcgccg
     6721 agttcgttgg cacgaattct ggagtttccg accgtagccg accacctcga cttgcgggag
     6781 tgcataagtc tcgccacccg cgtcccaggc aggccgggct ggcgcggcgc cacatggctg
     6841 gggcccgaac ggacacccga agccggaccg tcatctcgac cgggcgcgcg gcctgccgca
     6901 gctgaggccc cacctgcaac gcgtgctggc gcggccggcg acgcagcgtg cgtccgagcg
     6961 cgagggcctg gcgc
//
LOCUS       DATMJB010000500         3535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_557339, whole genome shotgun sequence.
ACCESSION   DATMJB010000500 DATMJB010000000
VERSION     DATMJB010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557339"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1148)
                     /locus_tag="VNU48_10760"
     CDS             complement(9..1148)
                     /locus_tag="VNU48_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWI11801.1"
                     /translation="MALIYYLTHIHLDFGSRTLLASECERVGINKPLIVTDAGVRAAG
                     VLQQVLDELPASLQGPVFDATPSNPTEAAVREAAALFRAHGCDGLIAVGGGSSLDCAK
                     GVAIAATHPGPLVTYATIEGGSAKITEAVAPLIAVPTTAGTGSEVARGAIVITDDHRK
                     LGFHSWHLLPRSAICDPELTLALPPGLTAATGMDAIAHCMETFMAPAFNPPADGIALD
                     GLERGWAHIERATRDGADREARLNMMSASIEGAMAFQKGLGAVHSLSHSLGGANPRLH
                     HGTLNAMFLPAVVRFNASADSVVKDKRLARMARAMGLASGGDIPDAIRAMNVRLGLPT
                     GLAAMGVEPAMFDRIVSGALADHCHKTNPRIASADEYRALIEASL"
     gene            1292..2491
                     /locus_tag="VNU48_10765"
     CDS             1292..2491
                     /locus_tag="VNU48_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HWI11802.1"
                     /translation="MFKALSAAGRYPFTALASAVVLTLAACGGGGASSGAGTLQMSLT
                     DAPACGYDAVNVTIAKLSVNQSGTASATDAGWVDIPITAQRIDLLTLQNGVLATLGQT
                     PLAPGKYTQMRLLLADNASAGAGQPIPNSVLPTGGSETALTTPSGQQTGLKLNVDIDI
                     AADKMADFVIDFNACKSIVSAGASGKYLLKPVVSVTPNFVSGVKGTVDASIASGTGTA
                     VMLEQPGTSTQAPVVVKATAPDSTGNYVLGPVAPGTYDLVVTSNGHATAVVTGVVVQD
                     GTVTTIGGAINPPLSATGTISGLVATGATPIDASLAVTQALTGGSTIELAGGPADSAS
                     GAYAFSLPVGAVSVAPYTGGALTFAADAAAPGKYTVAATSGGVTKPSSLLTVTAGATV
                     TAGFSFP"
     gene            2572..3462
                     /locus_tag="VNU48_10770"
     CDS             2572..3462
                     /locus_tag="VNU48_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HWI11803.1"
                     /translation="MPARNHPATSAPPELVDALAAVLRPLAQLCVAFGLPFAEVTEQL
                     KRACVDAARVGQADAARRVSRISTATGINRREVTRLLAAEPRPVAVRPRSRSVATEVF
                     AHWTTAREFRDRRGAPRALPRQGPGASFETLARSVTTDVHPRSILEQLVRLGLAELDP
                     KRDRVTLVRDAFVPRGDARRMFGFLGENVGDHLAGAVENVLGDGQRHFEQAVFADELS
                     EESMQQVRTLVTAQWRLLMQAMVPELERLIERDRIGAKTADRRLRIGLYTYEAQTLPA
                     RASDKPAAVPRSRRRKEPTT"
BASE COUNT          525 a         1269 c         1229 g          512 t
ORIGIN      
        1 gcccgcgttc agagcgacgc ttcgatcagc gcgcggtact cgtcggcgct ggcgatgcgc
       61 gggttggtct tgtggcagtg atcggccagc gcgccgctga cgatgcggtc gaacatcgcc
      121 ggctcgacac ccatcgccgc caggcccgtc ggcaggccca gccgcacatt catcgcgcgg
      181 atcgcgtcgg ggatatcgcc gccgctggcc agccccatcg cgcgcgccat tcgcgccagg
      241 cgcttgtcct tgacgacgct gtcggcgctg gcgttgaagc gcacgaccgc cggcaggaac
      301 atcgcgttga gcgtgccgtg gtgcagccgc gggttcgcgc cgcccaggct gtgcgacagc
      361 gaatgcaccg cgcccaggcc cttctggaac gccatcgcgc cctcgatcga cgcgctcatc
      421 atgttcagcc gcgcctcgcg gtccgcgccg tcgcgggtgg cacgctcgat gtgggcccag
      481 ccgcgctcca gcccgtccag cgcgatgccg tcggcgggcg ggttgaaggc cggcgccatg
      541 aaggtctcca tgcaatgcgc gatcgcgtcc atgccggtcg cggcggtcag gcccggcggc
      601 agcgcgagcg tcagctcggg gtcgcagatc gccgagcgcg gcagcagatg ccaggaatgg
      661 aagccgagct tgcggtggtc gtcggtgatc acgatcgcgc cgcgcgcgac ctcgctgccg
      721 gtgccggcgg tggtgggcac cgcgatcagc ggcgcaaccg cctcggtgat cttcgcgctg
      781 ccgccctcga tcgtcgcgta ggtcaccaac ggcccggggt gcgtggcggc gatcgccaca
      841 cctttcgcgc aatcgagcga cgagccgccg cccaccgcga tcagcccgtc gcagccgtgc
      901 gcgcgaaaca gcgccgcggc ctcgcgcacc gcggcctcgg tcgggttcga cggcgtggca
      961 tcgaagaccg ggccctgcag cgacgcgggc agctcatcga gcacctgctg cagcacgccg
     1021 gcggcgcgca cgccggcgtc ggtgacgatc aagggcttgt tgatgccgac gcgctcgcat
     1081 tcgcttgcga gcagcgtgcg gctgccgaag tccaggtgga tgtgcgtgag gtagtagatc
     1141 agtgccatcg tgcgactgta gcggcgcgcc ggcggtggga ttgatcaccg gtcattacaa
     1201 ctgctttctg aaatgtgcga attgcacatt tatgatgaaa ccacgacggc gcgccacgac
     1261 cgcatcgccc ttcatcctcc aggagaccct catgttcaaa gcactttccg ccgctggccg
     1321 atacccgttc accgcgctcg cgagcgccgt ggtactcacg ctggccgcct gcggcggcgg
     1381 cggcgcttcg agcggtgccg gcaccttgca gatgtcgctg accgacgcgc ccgcctgcgg
     1441 ctacgacgcg gtcaacgtga cgatcgccaa gctgagcgtg aaccagagcg gcaccgcgtc
     1501 tgccaccgat gcgggctggg tggacattcc gatcacggcg cagcgcatcg acctgctcac
     1561 gctgcagaac ggcgtcctgg ccacgctggg ccagacaccg ctggcccccg gcaaatacac
     1621 ccagatgcgt ctgctgctcg ccgacaacgc cagcgctggc gccggccagc cgatcccgaa
     1681 ctcggtgctg cccacgggcg gcagcgagac cgcgctgacc acgccgagcg gccagcagac
     1741 cgggctgaag ctgaacgtcg acatcgacat cgcggccgac aagatggcgg acttcgtcat
     1801 cgacttcaac gcctgcaagt ccatcgtgtc ggcaggcgcc tcgggcaagt acctgctcaa
     1861 gccggtcgtc agcgtcaccc cgaatttcgt ttcgggcgtg aagggaacgg tcgatgcctc
     1921 catagccagc ggcaccggca cggcggtgat gctggagcag cccggcacgt cgacccaggc
     1981 accggtcgtc gtcaaggcca ccgcgccgga cagcaccggc aactacgtcc tcgggccggt
     2041 ggcgccgggc acctacgatc tcgtcgtcac gtccaatggt cacgcgaccg cggtggtgac
     2101 cggcgtggtg gtgcaggacg gcacggtgac gaccatcggc ggcgccatca acccgccgct
     2161 ctcggccacc gggacgatca gcggtctcgt cgcgaccggc gcgacgccga tcgatgcctc
     2221 gctcgcggtg acgcaagcgc tgacaggagg cagcaccatc gagctggcgg gtggtccggc
     2281 ggactccgcg agcggcgcgt atgcattctc gctgccggtg ggcgccgtca gcgtcgcgcc
     2341 gtacaccggc ggcgcgctga ccttcgctgc cgatgcggcc gcgccgggca agtacaccgt
     2401 cgcggcgacc tcgggcggcg tcacgaagcc atcgagcctg ctcacggtca ccgcaggcgc
     2461 cacggtgacg gcggggttca gcttcccttg atgccgcggc gattcctgta gcctcgcgcc
     2521 ggggtcccct tgggtctccg gcgcttttct attccggacc ccgacgctgc catgcccgcc
     2581 cgcaaccacc ccgccaccag tgcaccgccc gagttggtcg atgcgctggc cgcggtgttg
     2641 cgcccgctcg cgcagctgtg cgtcgcgttc ggcctgccgt tcgccgaagt gaccgagcag
     2701 ctgaagcgcg cctgcgtcga cgcggcgcgc gtcggccagg ccgatgcggc ccggcgcgtg
     2761 agccgaatca gcaccgccac cggcatcaac cggcgcgagg tcacgcggct gctggccgcc
     2821 gagccgcggc cggtcgcagt gcgaccacgc agccgctcgg tggccaccga ggtcttcgcg
     2881 cactggacca cggcgcgtga gttccgcgac cgccgcggtg cgccgcgcgc cctgccgcgg
     2941 cagggcccgg gcgcgagctt cgagaccctg gcgcggtcgg tgaccaccga tgtccatccg
     3001 cgcagcatcc tcgagcaact ggtacgcctg ggcctggccg aactcgaccc gaagcgcgac
     3061 cgcgtcacgc tggtgcgcga cgcgttcgtg ccgcgcggcg atgcgcggcg gatgttcggc
     3121 ttcctcggcg agaacgtcgg ggaccatctc gccggcgcgg tcgagaacgt gctcggcgac
     3181 ggccagcgcc acttcgagca ggccgtgttt gccgacgaat tgtcggaaga atccatgcag
     3241 caggtgcgca cgctggtcac ggcgcagtgg cgattgctca tgcaggccat ggtgcccgag
     3301 ctcgaacgac tgatcgagcg cgatcgcatc ggcgcaaaga ccgcagaccg gcggctgcgg
     3361 atcggcctct acacgtatga agcgcagaca ctgccagcgc gcgccagcga caagcccgcg
     3421 gcggtgccga ggtcgcgtcg ccgcaaggag ccaacgacat gatcaggatt ctcgatcgct
     3481 gcggcgcgac ccgcgcagga gtgaggggca tcggcgcagg cctggcgctg gcatg
//
LOCUS       DATMJB010000501         2159 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_516250, whole genome shotgun sequence.
ACCESSION   DATMJB010000501 DATMJB010000000
VERSION     DATMJB010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516250"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1816)
                     /gene="cas8c"
                     /locus_tag="VNU48_10775"
     CDS             complement(44..1816)
                     /gene="cas8c"
                     /locus_tag="VNU48_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HWI11804.1"
                     /translation="MILQELVRYYDRKSRDPDPAQRLPNFGLEDKEIPFVIELAADGR
                     VVQLRDTRSLEGKKLRAKSFLVPQGVKKTSAVAANLLWDSAAYAIGLDKSRKSKAEVT
                     PHAAFRARIEALPEAAKADAGPRAVLAALDRADWSVLQGHPAWAEIQETGPVMTFQLS
                     GDVDLICQRPAVAAAALPMADADAKPGLCLVEGVCAPIQRLHSAIKGVWGAQSSGANI
                     VSFNARAFESYGKGERQGENAPVGERAAFAYTTALNHLLAKDSRNRVQVGDASTVFWA
                     DTASRFDSEFTLADFFGETKDDPDRGVRAVQALHQALASGQLPVGERDAQFFVLGLAP
                     NAARISVRFWMRAPLSELAPRILQHFADLKVARRYDSDSTTPSLFRLLSSVALLGKLD
                     NAPPRLAGEWMRAILEGQPYPATLLNAAVIRCKAEREVTTLRAAVLKAWLNRDHRRGH
                     PAAPAHLQHFKEELDMQQTDVPYLLGRLFAVLERIQQQAQPGINATIRDRYYGAASTT
                     PVSVFTTLLRLKNAHLKKLGDGQTAHFERLIGDILLPLTDFPRQLALPQQAKFALGYY
                     HQRQAFFTRKEEASADNTPPAQET"
     gene            complement(1813..>2159)
                     /gene="cas5c"
                     /locus_tag="VNU48_10780"
     CDS             complement(1813..>2159)
                     /gene="cas5c"
                     /locus_tag="VNU48_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HWI11805.1"
                     /translation="LLLRDVAYRVHADLEALADRQDPMPPQKYHAIFERRAENGQWVN
                     QPYLGCREFAARVRLVTDPTGEPAPIGETRDLGWMLHDLDFSDPADPQPVFFRAQMNA
                     GVIEVGTQGAAR"
BASE COUNT          354 a          683 c          760 g          362 t
ORIGIN      
        1 tcgaacaaca gcacgaagtc atagcgagag gtgagcggca tggtcaggtc tcctgggcgg
       61 gtggggtgtt gtcggcgctg gcttcttcct tgcgcgtgaa gaaggcctgc cgctggtggt
      121 agtagccgag tgcgaacttg gcctgctgcg ggagcgcgag ctggcgcggg aaatcggtga
      181 gcggaagcag gatgtcgccg atgagccgct cgaaatgagc ggtttgcccg tcaccgagtt
      241 tcttgaggtg ggcgttcttg aggcgcagca gtgtggtgaa caccgatacc ggtgtggttg
      301 acgcggcgcc gtagtagcgg tcgcgaatcg tggcgttgat gccgggctgc gcctgttgct
      361 ggatgcgctc cagcacggca aacagtcggc ccaacagata gggcacatcg gtctgctgca
      421 tgtccagctc ctctttgaaa tgttgaaggt gcgcgggcgc tgcggggtgg ccgcggcggt
      481 ggtcgcggtt gagccaggcc ttgagcacgg ccgcacgcag agtggtgacc tcccgctccg
      541 ccttgcaccg gatcactgcc gcattgagca gcgtggccgg gtacggttga ccctccagaa
      601 tggcgcgcat ccactcgccc gccaggcgag ggggcgcgtt gtcgagcttg cccagcagcg
      661 ccacgctgga gagcagccgg aacagcgagg gggtggtgga gtcgctgtcg tagcggcgtg
      721 ccaccttcaa atcggcgaag tgctgcagga tgcggggtgc cagctcggac agcggcgcac
      781 gcatccagaa gcggacgctg attcgggccg cattcggcgc gaggccgagc acgaagaatt
      841 gcgcatcccg ttcgcccacg ggcaactggc cgctcgccag cgcctggtgc agggcctgaa
      901 ctgcgcgcac gccacggtcg ggatcgtcct tggtctcgcc gaagaaatcg gccagcgtga
      961 actcgctgtc gaaccgcgac gcggtgtcgg cccagaacac cgtggatgcg tcgcccacct
     1021 gcacgcggtt gcgcgagtct ttggcgagca ggtggttcag cgcggtggtg taggcgaacg
     1081 cggcacgctc gccgacaggt gcgttctcgc cctggcgttc gcccttcccg taagactcga
     1141 atgcacgggc gttgaaggac acgatgttgg cgcccgaaga ctgcgcaccc caaacgccct
     1201 tgatggccga gtgcagccgc tggatcgggg cgcaaacgcc ttcgaccagg cacaagcccg
     1261 gcttcgcatc ggcatcggcc atcggcaacg ccgcggccgc cacggccggc cgctggcaga
     1321 tcagatcgac atcgccagag agctggaatg tcatgaccgg gccggtctcc tggatctcgg
     1381 cccaggccgg gtggccctgc agcacggacc aatcggcgcg atcgagcgca gcgagaacgg
     1441 cgcgcggacc agcgtcagct ttcgcggcct cgggcagggc ttcgatgcgc gcgcgaaacg
     1501 ccgcgtgagg tgtgacttcg gccttgctct tgcgcgactt gtcgaggccg atcgcgtaag
     1561 ctgcgctgtc ccacagcagg ttcgcggcca cggccgacgt cttcttgacc ccctggggca
     1621 ccaggaacga cttggcgcgc aacttcttgc cctccaggct tcgggtgtcg cgcaactgga
     1681 ccacgcgacc gtctgcggcc agctcgatga caaacggaat ctccttgtct tccagaccaa
     1741 aattgggcag gcgttgcgcg ggatcggggt cgcgcgactt gcggtcgtag tagcgcacca
     1801 actcctgaag aatcatcgcg cagctccctg cgtgccgacc tcgatgacgc ccgcgttcat
     1861 ctgcgcgcgg aagaacaccg gctgcggatc ggcgggatcg ctgaaatcca ggtcgtgcag
     1921 catccagccc agatcgcgcg tctcgccgat gggcgcgggc tcgccggtcg ggtcggtcac
     1981 caagcgcacc cgtgccgcga actcgcgaca gccgaggtag ggctgattga cccactgccc
     2041 gttctcggca cgccgctcga agatggcgtg gtacttctgc ggcggcatcg ggtcttgtcg
     2101 atcggccagg gcctccaggt cggcgtgcac gcgataggcc acgtcacgca gcagcaggc
//
LOCUS       DATMJB010000502         2306 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_138719, whole genome shotgun sequence.
ACCESSION   DATMJB010000502 DATMJB010000000
VERSION     DATMJB010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138719"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..465
                     /gene="pedF"
                     /locus_tag="VNU48_10785"
     CDS             13..465
                     /gene="pedF"
                     /locus_tag="VNU48_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018314666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-550 PedF"
                     /protein_id="HWI11806.1"
                     /translation="MRTLLLVTAALVACAAAWAHGDVTPQAVDTTGLPRLGEAWRDEN
                     PFRSNELAIKIGNSGYNQNCARCHGLEVISGGIAPDLRKLDNECTTLKDGKRGACVKE
                     VDDYFVSTVRKGRTRNGAVYMPPFEGVLDQEAMWSIKAYLETRRDKPL"
     gene            501..1343
                     /locus_tag="VNU48_10790"
     CDS             501..1343
                     /locus_tag="VNU48_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HWI11807.1"
                     /translation="MNPTRRDCLRRLGAAGAAALAASPLALSATPLTRVQERGVLTVG
                     LYHDMPPFHVDGKGIDVELAQALAASLGVKLALLPFHADDNMNDDLRNMVWRGHYLGY
                     GPADVLLHVPVDRPLMDGNPQVSVFAPYCRERVAIARRVDLVPRLDTFSQLDGRMIAV
                     PGQSLAGWLLIGADGGAYRSQLNTQWKDGAQAAQALLRGEVQAAAGLASELESVLRDD
                     ARFAISPLPSPRAPRDGWAVGMAVKRDATELAQALQRGVGELAATGRLREMFARANLL
                     WQAA"
     gene            complement(1388..1696)
                     /locus_tag="VNU48_10795"
     CDS             complement(1388..1696)
                     /locus_tag="VNU48_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWI11808.1"
                     /translation="METRDERGVLESWLVRASGRVQGIGYRQACVSRARALGIGGWVR
                     NRLDGSVEALLQGSPEQLAQMCHWLRDGMPAALVDDLEVAQTPSPFPRFDGFDCLPTL
                     "
     gene            complement(1743..>2306)
                     /locus_tag="VNU48_10800"
     CDS             complement(1743..>2306)
                     /locus_tag="VNU48_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11809.1"
                     /translation="HRLHCAARSGVAPQNSLRSLRSLRSNSRGESVYEARAARAPTRW
                     WSLWGAVDLVPLDDKVLVANPALVNPLQDATELAVDGPAQGRVVLAGGFGSHGEAVRM
                     ERDARGRPTAFWLAGTRLRPEAAIAKELMAKYERPRHARAAARGTRRIHRNRPRLASN
                     RRHQFPVGLARHLWMRWGDAGLGRPAE"
BASE COUNT          356 a          824 c          787 g          339 t
ORIGIN      
        1 ggacggccgc gcattcgcac cttgctgctc gtgaccgccg cgctcgtcgc ttgcgccgct
       61 gcatgggccc acggcgacgt cacgccgcag gccgtcgaca ccaccggcct gccgcggctc
      121 ggcgaagcct ggcgcgacga gaacccgttt cgcagcaacg agctggcgat caagatcggc
      181 aactcgggct acaaccagaa ctgcgcgcgc tgccatggcc tcgaagtgat ctccggcggc
      241 atcgcacccg acctgcgcaa gctcgacaac gagtgcacga cgctgaagga cggcaagcgc
      301 ggcgcctgcg tgaaggaggt cgacgactat ttcgtctcga ccgtccgcaa gggccgcacg
      361 cgcaacggcg cggtctacat gccgccgttc gaaggcgtgc tcgaccagga agcgatgtgg
      421 tcgatcaagg cctatctcga aacgcggcgc gacaagccgc tgtgacgcgc gctccgaccg
      481 ggagaaccac catgcgactc atgaacccga cacgtcgcga ctgcctgcgc cggctcggcg
      541 cggctggcgc cgcggcgttg gcggcctcgc cgctcgcgct gtcggccacg ccactgacac
      601 gcgtgcagga gcgtggcgtc ctcacggtcg gcctctatca cgacatgccg ccgtttcacg
      661 tcgacggcaa ggggatcgac gtcgaactgg cgcaagcgct cgccgcatcg ctgggcgtga
      721 agctcgcgct gctgccgttc catgcggacg acaacatgaa cgacgatttg cgcaacatgg
      781 tctggcgcgg ccactacctc ggctacggcc cggccgacgt gctgctgcac gtgccggtcg
      841 atcggccgct gatggacggc aacccgcagg tcagcgtgtt cgcgccctac tgtcgcgagc
      901 gggtcgcgat cgcgcgccgg gtcgatctcg tcccgcgcct cgacacgttc tcgcaactcg
      961 atggccggat gatcgccgtg cctggccagt cgctcgcagg ctggctgctg atcggtgcgg
     1021 acggtggcgc ctaccggagt cagctgaaca cgcagtggaa agacggcgcg caggccgcac
     1081 aggcgctgct gcgcggcgag gtgcaggcgg ccgccgggct ggcctcggaa ctcgaatcgg
     1141 tgttgcgcga cgatgcgcgc tttgcgatct cgccgctgcc gtcgccacgc gcgccgcgtg
     1201 acggctgggc cgtcggcatg gcggtcaagc gcgacgccac cgagctggcg caggcgctgc
     1261 agcgtggcgt cggcgagctc gccgcgaccg ggcgcctgcg ggagatgttc gcgcgcgcga
     1321 acctgctgtg gcaagcggcg tgatggaccg atcctgcggc ggcgcggcgc gaaaccgcac
     1381 cgtgccctca cagcgtgggc aagcagtcga agccgtcgaa gcgagggaat ggcgacggtg
     1441 tctgcgctac ttcaaggtcg tcgacgagtg ctgcgggcat tccatcgcgc aaccaatggc
     1501 acatctgcgc aagctgctct ggtgacccct gcagcaacgc ctcgaccgac ccgtccaagc
     1561 ggttgcggac ccagccgccg atcccgagtg cccgcgcgcg gctcacgcac gcttggcgat
     1621 agccgatgcc ttgcacgcgg ccagacgccc gcaccagcca gctctcgagc acccctctct
     1681 cgtcgcgagt ttccatgcgg cactcccact gcaaagtcat gccctgcgcg gacgattcaa
     1741 tcttactcag ctggtcgacc caggccggca tcaccccatc gcatccaaag gtgtcgggcg
     1801 agcccgacag gaaactgatg ccggcggttg ctcgcgagtc gcgggcggtt acggtggatc
     1861 cgtcttgtgc cccgtgccgc ggcgcgcgca tgtcgcgggc gctcgtactt cgccatcaat
     1921 tccttcgcga tcgccgcctc gggccgcaag cgcgtgccgg cgagccagaa cgcggtcggt
     1981 cgaccgcgcg cgtcacgctc catgcgcacc gcttcgccgt ggctgccaaa gccgccggcg
     2041 agcacgacgc ggccctgggc cggcccgtcg acggcgagtt cggtggcgtc ctgcagcggg
     2101 ttgacgagcg ccgggttcgc gaccagcacc ttgtcgtcca gcggcaccag atcgaccgca
     2161 ccccacaggc tccaccagcg ggtcggcgcg cgcgcagcgc gcgcttcgta gactgactcg
     2221 ccgcggctgt ttgaacgcag cgaacgcagt gagcgcagtg agttctgcgg cgcgaccccg
     2281 gaccgagcag cgcagtgcag tcggtg
//
LOCUS       DATMJB010000503         5225 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_193481, whole genome shotgun sequence.
ACCESSION   DATMJB010000503 DATMJB010000000
VERSION     DATMJB010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193481"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VNU48_10805"
     CDS             complement(<1..589)
                     /locus_tag="VNU48_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HWI11810.1"
                     /translation="MASTLTDRLSRLVKTMRGQARITEDNVQDMLREVRMALLEADVA
                     LPVVRDFIARVKDKALGAEVVGSLSPGQALVGIVNRELAATMGEGVSDINLAAQPPAV
                     ILMAGLQGAGKTTTTAKLAKHLIEKRKKKVLTVSADVYRPAAIEQLKLVTKQAGAEWF
                     PSSGADQPLAIGLAALDYARKHYFDVLLVDTAGRLG"
     gene            653..1501
                     /gene="ccsA"
                     /locus_tag="VNU48_10810"
     CDS             653..1501
                     /gene="ccsA"
                     /locus_tag="VNU48_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HWI11811.1"
                     /translation="MILSLAPALTPTAAGASPSGWVLGLPSALALAAYAAAALLPERL
                     ADALRAALLGAFVAHAVAIVVDISGLSSHVEVARFGFAPALSMTVWLVLAVYLVESRF
                     VPLPGARRMLALLGIVVVALAWTFPGEVRPQVDSRWAPLHWALGIASYGLFGAAVLHA
                     VMLNRAERQMRPSAGAIAAPPRGMPLLQLERMTFRFVGAGFIVLSAALLLGAWFAHPW
                     RWDHKTVFSVLGWLVFAGLLAGRRAFGWRGPVATRGVYVGAGLLLLAYVGSRFVLEVL
                     LHRAPA"
     gene            1517..1759
                     /locus_tag="VNU48_10815"
     CDS             1517..1759
                     /locus_tag="VNU48_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP0621 family protein"
                     /protein_id="HWI11812.1"
                     /translation="MKFLLLLVAVFVLLWLLRGSLQRARRPPSAPKPGAEAPQPMLSC
                     AHCGLHLPRDEALPGRGGVFCGAAHRAAYEQAHPDS"
     gene            1756..3711
                     /locus_tag="VNU48_10820"
     CDS             1756..3711
                     /locus_tag="VNU48_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI11813.1"
                     /translation="MNSADRASERRFADRRRMRRSTAADESWFGAVGAGADADAQVRD
                     DDLPDDDAPATAGPDEAAHAKSGWAGDSRFLSRTAVHVVESGQTAFERLYRAFIAARA
                     ALGLALLVALVLGGLFGLRPTPIVTLISAAYAALAIGLWLLPRYRRPAVPRVMAHLRG
                     PQWLATIGVDLVSFMALHVLAPGSSLNYVALLVLPVLMAGVLTPRTMALATAAAVTLT
                     LLGAAWASVLAGAEGTLQMTQAGLAGTGFFVITVLAGELAGRLAREELTARGSLEMAR
                     QQAQLNRLVIEEMQDGVLVVDRRGRVRAANPAARRLLAPSGMSRPAPFQLRGVQAWDA
                     LVKTVERAFGEATWPEAGRDVVLLFENDTPSALGVQRTLRVRVRFTRKREPQTIEEFC
                     VLFLEDVRNMQARSRQEKLAAMGRVSAGIAHEIRNPLAAISQANALLSEDATDPAQRQ
                     LTQMVSDNVERLKRIVDDVMEVAPGAVQDVGVIDATAQVAAVCSEWARATGVALGEHS
                     VLRVELPTEPLGVLFDAEHLRRVLVNLLDNAYRHASRAPGAIQLRLDSRREAQAFLSL
                     ASDGALIPPDVEPFLFEPFFSTRSRGTGLGLYICRELCERYGATIDYRLRAEGVAQRN
                     EFFVEMQRKALGPNSVEPATGTVARPP"
     gene            3708..>5225
                     /locus_tag="VNU48_10825"
     CDS             3708..>5225
                     /locus_tag="VNU48_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWI11814.1"
                     /translation="MNNPSHFSLLVVDDEPDLRTLYELTLLREGYDIETASTVQDALL
                     HLKDRTYSAVITDMRLPDGTGLDVLRWLEESGRREKTIVITAYGSAENAVEALKAGAY
                     DYLTKPVDLKQFRAVVASALGRPGGSGGPTVDPATLPSESGAAPTATARPAPVAQAPA
                     APVSQALNRMAGQSSAMQQVRSLVDKVARSMAPVMVNGESGTGKELVARAIHEVSVRS
                     AQPFIAVNCSAIPEQLLEAEFFGYRKGAFTGAMEDREGVFQAANGGTLFLDEIGDLPL
                     PMQSKLLRAVQERSVRPVGAVAEVPVNVRLLSATHKDLAAEVHAGTFRQDLYYRLNVI
                     QIRVPPLRERLEDLVAISERVLERIAHDAGVPTPPRLTRDALVHLERYPFPGNVRELE
                     NLLHRAVALSGGEVIDVYDLGLPESVFTDSAAQELDLITENTRGDVLQAVRELAPPVP
                     VEVPLPNDLERHLDQVERDILVRALERHRFNRTAAGASLGLSLRQMRYRMARLGVK"
BASE COUNT          676 a         1850 c         1896 g          803 t
ORIGIN      
        1 cgcccaagcg accggcggtg tcgaccagca gcacgtcgaa atagtgcttg cgcgcgtagt
       61 cgagtgcggc caggccgatc gccagcggct ggtccgcgcc gctggacggg aaccattcgg
      121 cgcccgcctg cttcgtcacc agcttgagtt gctcgatcgc ggccgggcgg tagacgtcgg
      181 cagaaaccgt cagcaccttc ttcttgcgct tctcgatcag gtgcttcgcg agcttcgcgg
      241 tcgtcgtggt cttgccggcg ccttgcaggc cggccatcag gatcaccgcg ggcggctgtg
      301 cggcgaggtt gatgtcggac acgccttcgc ccatggtcgc ggcgagttcg cggttgacga
      361 tgcccaccag cgcctgcccc ggcgacagcg aaccgacgac ctcggcgccg agcgccttgt
      421 ccttcacccg cgcgatgaag tcgcgcacca ccggcagcgc gacgtcggct tcgagcagcg
      481 ccatgcggac ctcgcgcagc atgtcctgca cgttgtcttc ggtgatgcgg gcctggccgc
      541 gcatcgtctt gaccaggcga ctgagccggt cggtgagggt ggaagccatc gaggtggggg
      601 cgcgcccggg cgccgaagct tcggccagca ggcgcgaaag tacactgtga cgatgattct
      661 atcgttggcc cctgcactca cccccacggc cgcgggcgca tccccgtcgg gttgggtgct
      721 cgggctgccg agcgcactcg cgcttgcggc ctacgccgcc gcggcgctgt tgccggagcg
      781 cctggccgac gccttgcgcg ctgccctgct cggcgccttc gtcgcgcatg cggtggcgat
      841 cgtcgtcgac atctcgggcc tgagcagcca tgtcgaggtc gcgcgtttcg ggttcgcgcc
      901 ggcgctgtcg atgacggtct ggctggtgct cgcggtctac ctggtcgaga gccgcttcgt
      961 gcccttgccg ggcgcgcgcc gcatgctcgc gctgctgggc atcgtcgtgg tggcgctggc
     1021 ctggaccttc cccggcgagg tgcgcccgca ggtcgattcg cgctgggcgc cgctgcactg
     1081 ggcgctcggg atcgcatcgt acggcctgtt cggcgcggca gtgctgcacg cggtgatgct
     1141 caaccgcgcc gagcgccaga tgcggccgtc ggccggtgcg atcgccgcgc cgccgcgagg
     1201 catgccgctg ctgcagctcg agcgcatgac gttccgcttc gtcggcgccg gcttcatcgt
     1261 gctgtcggcg gcgctgctgc tcggtgcctg gttcgcgcac ccctggcgct gggaccacaa
     1321 gaccgtgttc tcggtgctcg gctggctcgt gttcgccggg ctgctcgccg gccggcgcgc
     1381 gttcggctgg cgcgggccgg tggccacgcg cggcgtgtat gtcggtgcgg gcctgctgct
     1441 gctggcctac gtgggctcgc gcttcgtgct tgaggtgctg ctgcaccgcg caccggcatg
     1501 atcatcgggt gagcgcatga aattcctgct gttgctcgtc gccgtgttcg tgctgctgtg
     1561 gctgctgcgc ggcagcctgc agcgcgcgcg ccgcccgccc tccgcgccga agcccggcgc
     1621 cgaagcgccg caaccgatgc tcagttgcgc ccactgcggc ctgcacctgc cgcgcgacga
     1681 ggcgctgccg ggtcgcggcg gtgtcttctg cggtgccgcg caccgcgcgg cgtacgagca
     1741 ggcgcacccg gattcatgaa cagcgctgac cgcgcgtcgg agcgccgctt cgcggaccgg
     1801 cgccgcatgc gtcggtccac tgcggccgac gaatcctggt tcggtgccgt cggcgccggc
     1861 gccgacgccg acgcccaggt gcgcgacgac gacctgcccg acgacgacgc gcccgcgaca
     1921 gccggccccg acgaggcagc gcacgcgaag tccggttggg ccggcgactc gcgctttctg
     1981 tcacgcacgg cggtgcatgt cgtcgagtcg gggcagacgg cgttcgaacg cctgtaccgc
     2041 gccttcatcg cggcccgtgc cgcgctcggg ctcgcgctgc tcgtggcgct ggtgctgggt
     2101 ggcctgttcg ggctgcgccc gacgccgatc gtcacgctga tcagcgcggc ctacgccgca
     2161 ctcgcgatcg gtctgtggct gctgccgcgc taccggcggc ccgccgtgcc gcgcgtgatg
     2221 gcgcatctgc gcggcccgca atggctcgcg acgatcggcg tcgacctggt gtccttcatg
     2281 gcgctgcacg tgctcgcgcc cgggtcgagc ctgaactacg tcgcgctgct ggtcctgccg
     2341 gtgctgatgg ccggcgtgct gacgccgcgc acgatggcgc tggccaccgc cgcggcggtg
     2401 acgctgacgc tgctcggcgc tgcctgggcc agcgtcctcg cgggcgccga aggcaccttg
     2461 cagatgacgc aggctggcct cgccggcacc ggcttcttcg tcatcacggt gctggccggc
     2521 gaactcgccg ggcgcctggc gcgtgaggag ctgactgccc gcggcagcct cgagatggcg
     2581 cgccagcagg cgcagctgaa ccggctcgtg atcgaggaga tgcaggacgg cgtgctggtc
     2641 gtcgaccggc gcggccgcgt gcgcgctgcc aaccccgcgg cgcgccgcct gctcgccccc
     2701 agcggcatga gtcggcccgc gccgttccag ttgcgcggcg tgcaggcctg ggacgcgctg
     2761 gtgaagacgg tcgagcgcgc gttcggcgaa gcgacctggc ccgaggccgg ccgcgacgtg
     2821 gtgctgctgt tcgagaacga cacgccgagc gcgctcggcg tgcagcgcac cttgcgcgtg
     2881 cgggtgcgtt tcacgcgcaa gcgcgaaccg cagacgatcg aggaattctg cgtgctgttc
     2941 ctggaggacg tgcgcaacat gcaggcccgc agccgccagg agaaactcgc ggcgatgggt
     3001 cgcgtgtcgg ccggcatcgc gcacgagatc cgcaacccgc tggcggcgat ctcgcaggcg
     3061 aacgcgctgc tgtccgagga cgccaccgac ccggcgcagc gccagctgac gcagatggtc
     3121 agcgacaacg tcgagcgcct gaagcgcatc gtcgacgacg tgatggaggt ggcgcccggt
     3181 gccgtgcagg acgtcggcgt gatcgacgcg accgcgcagg tcgccgcggt gtgcagcgaa
     3241 tgggcgcgcg cgaccggcgt cgcgctcggg gagcacagcg tgctgcgcgt cgaactgccg
     3301 accgagccgc tcggcgtgct cttcgatgcc gagcatctgc gccgcgtgct ggtgaatctg
     3361 ctcgacaacg cgtatcgcca cgccagccgc gcgccggggg cgatccagct gcggctcgac
     3421 tcgcgccgcg aagcgcaggc gttcctgagc ctggccagcg acggcgcgct gatcccgccc
     3481 gacgtcgagc cgttcctgtt cgagccgttt ttctcgaccc gcagccgcgg caccggcctg
     3541 ggcttgtata tttgccggga actgtgcgag cgctacggcg ccaccatcga ctaccggctg
     3601 cgtgccgaag gtgtggccca gcgcaacgag ttcttcgtcg agatgcagcg caaggcactg
     3661 ggtccgaact ctgttgagcc agccaccggc accgttgccc gcccgccatg aacaacccat
     3721 cgcacttcag cctgctggtc gtcgacgacg agcccgatct gcgcacgctc tacgagctga
     3781 ccctgctgcg cgagggctac gacatcgaga ccgcgagcac ggtccaggac gcgctgctgc
     3841 acctgaagga ccgcacctac agcgccgtca tcaccgacat gcgcctgccc gacggcaccg
     3901 gcctggacgt gctgcgctgg ctcgaggaga gcgggcgccg cgagaagacg atcgtgatca
     3961 ccgcgtacgg ctcggccgaa aacgcggtcg aggcgctgaa ggccggcgcg tacgactacc
     4021 tgaccaagcc ggtggacctg aaacagttcc gtgcggtcgt ggcctcggcg ctcggccgcc
     4081 cggggggcag cggcgggccg acggtcgacc cggccacgct gccgtcggaa agcggcgccg
     4141 cgccgaccgc cacggcgcgt ccggcgccgg tcgcacaagc accggccgcg ccggtgagcc
     4201 aggcgctgaa ccggatggcc ggccagtcga gcgcgatgca gcaggtgcgc tcgctggtcg
     4261 acaaggtcgc gcgcagcatg gcgccggtga tggtcaacgg cgaatcgggc accggcaagg
     4321 aactggtggc gcgtgccatc cacgaggtca gcgtgcgcag cgcgcagccg ttcatcgcgg
     4381 tgaactgcag cgcgatcccg gagcaactgc tcgaggccga gttcttcggc taccgcaagg
     4441 gcgcgttcac cggcgcgatg gaagaccgcg aaggcgtgtt ccaggccgcc aacggcggca
     4501 cgctgtttct cgacgagatc ggcgacctgc cgctgccgat gcagtcgaag ctgttgcgcg
     4561 cggtgcagga acgctcggtg cggccggtcg gtgccgtcgc cgaggtgccg gtcaacgtgc
     4621 gcctgctcag cgccacccac aaggacctgg ccgccgaagt gcacgccggc acctttcgcc
     4681 aggatctgta ctaccggctc aacgtgatcc agatccgcgt gccgccgctg cgcgagcggc
     4741 tcgaggacct ggtcgcgatc agcgagcgcg tgctcgagcg catcgcgcac gatgccggtg
     4801 tcccgacgcc gccgcgcctg acgcgcgacg cgctggtgca cctggagcgc tacccgttcc
     4861 cgggcaatgt gcgcgagctg gagaacctgc tgcaccgcgc ggtcgcgctg tcgggcggcg
     4921 aggtcatcga tgtctacgac ctcgggctgc ccgagtcggt gttcacggac agcgcggcgc
     4981 aggagctcga cctgatcacc gagaacacgc gcggcgacgt gctgcaggcg gtgcgcgagc
     5041 tggcgccgcc ggtgccggtc gaggtgccgc tgccgaacga cctggagcgc catctcgatc
     5101 aagtggagcg cgacatcctc gtgcgtgcgc tcgaacggca tcgcttcaac cgcaccgcgg
     5161 cgggggcgag cctcgggctg tcgctgcgcc agatgcgcta tcgcatggcg cggctcggcg
     5221 tcaaa
//
LOCUS       DATMJB010000504         3028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_375552, whole genome shotgun sequence.
ACCESSION   DATMJB010000504 DATMJB010000000
VERSION     DATMJB010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375552"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VNU48_10830"
     CDS             <1..393
                     /locus_tag="VNU48_10830"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWI11815.1"
                     /translation="ARPLKTPEDLRHQVLMYLDSGRGAGADMQDWPIWLRAMKLEGLK
                     PASVLHFSQFDQLINAAVAGQGVALGRIPLLKQLLRERKLVAPFKKTIASPRSYYLMQ
                     SAGAARKPEVQDFAAWLRDEARASALPD"
     gene            complement(409..1809)
                     /locus_tag="VNU48_10835"
     CDS             complement(409..1809)
                     /locus_tag="VNU48_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWI11816.1"
                     /translation="MTLVEALRAAVGAAHVLTDGDLSAYEVDWRKRYRGRALAVVRPA
                     STAEVAAVVTTCAAHGAAIVAQGGNTGLVGASVPDASGTQVLLNLARMNRVRAIDAEN
                     LTMTVDAGCVLQVVQEAAAAHGLLFALSLAAEGSCTIGGNLATNAGGTGVLRYGNARE
                     LCLGLEVVTATGEVWEGLSGLRKDNTGYDLRDLFIGSEGTLGIITGATLKLHPQPAAV
                     TTALATLSTLEAAVGLLQLARTRLGAGLTGFEVMNRHALALVRSHLPQLTQPLPPSDW
                     TVLLEQSDSESEQHARALFETLLETALERGLIGDAAVASSLEQSKAMWHLREAIPLAQ
                     ALEGANIKHDIALPVSTVARFVARTDALLLHAFPGVRLVDFGHLGDGNLHYNVQAPDG
                     VDAAAFVRDREAAVNALVYDSVAAFGGSISAEHGIGALKRDELAARKSPVALQLMRAI
                     KHALDPAGLLNPGRVL"
     gene            complement(1806..2204)
                     /locus_tag="VNU48_10840"
     CDS             complement(1806..2204)
                     /locus_tag="VNU48_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HWI11817.1"
                     /translation="MTDAPIALPARAALPAPIAWTRAVALASVLALIALGLAWELWLA
                     PTGSRTLALKVLPLLLPLPGLWRNRMYTYRWLSLMVWLYFTEGVVRATSDRGAGAALG
                     AIELLLCLLLFAACAAHVRWRLRHAKEPAA"
     gene            2225..>3028
                     /locus_tag="VNU48_10845"
     CDS             2225..>3028
                     /locus_tag="VNU48_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HWI11818.1"
                     /translation="MKFTTTIESALHEQLPDLVQTLQTWPWLDTLRTLRARFREDRLG
                     VTASSLTFTTLISLVPLLTVMLAVFSAFPMFASFQDALQKYFLQTLVPSDIANQVLRA
                     LNQFAGKAKGVGSVGLVLLFLAALALMLTIDRTLNGIWRVRNPRPIAQRVLVYWAAAT
                     LGPLVLGVSLSLTSYALSASQGLVGVLPGGVSLLLGTLEFLLLVAALSGLFHYVPNTH
                     VRWRHAFAGGVFVAIGIVGVKRLLALYFSTVPTYSMIYGAFATVPIFLIW"
BASE COUNT          411 a         1093 c         1070 g          454 t
ORIGIN      
        1 gcgcgcccgc tgaagacgcc cgaggacctg cgccaccagg tgctgatgta cctcgactcc
       61 ggccgcggcg ccggcgccga catgcaggac tggccgatct ggttgcgcgc gatgaagctc
      121 gaggggctga aaccggccag cgtgctgcac ttctcgcagt tcgatcagct gatcaacgcg
      181 gccgtcgccg ggcagggcgt cgcgctcggg cgcatcccgc tgctcaagca gctgctgcgc
      241 gagcgcaagc tggtcgcgcc gttcaagaag accatcgcgt cgccgcgcag ctactacctc
      301 atgcagtcgg ccggcgcggc gcgcaagccc gaggtgcagg acttcgccgc gtggctgcgc
      361 gacgaggcgc gcgccagcgc gctgcccgac tgatcccgtc tcgcgacgtc agagcacgcg
      421 accggggttg agcagcccgg ccgggtcgag cgcgtgcttg atcgcgcgca tcagctgcag
      481 cgccaccggc gacttgcgcg ccgcgagctc gtcgcgcttc agcgcgccga tgccgtgctc
      541 ggccgagatc gagccgccga aggccgcgac gctgtcatac acgagcgcgt tgaccgccgc
      601 ctcgcgatcg cgcacgaacg cggccgcatc gacgccgtcg ggcgcctgca cgttgtagtg
      661 caggttgccg tcgccgaggt ggccgaagtc gaccagccgc acgccgggga acgcgtgcag
      721 cagcagcgca tcggtgcgcg cgacgaagcg cgcgacggtc gacaccggca gcgcgatgtc
      781 gtgtttgatg ttcgcgcctt ccagggcctg cgccagcggg atcgcctcgc gcagatgcca
      841 catcgccttc gattgctcga ggctgcttgc gaccgccgca tcgccgatca ggccgcgttc
      901 gagcgcggtc tcgagcagcg tctcgaagag tgcacgggcg tgctgttcgc tctcgctgtc
      961 ggactgctcc agcagcaccg tccagtcgct gggcggcagc ggctgggtca gctgcggcag
     1021 gtggctgcgc accagcgcga gcgcgtggcg gttcatcacc tcgaagccgg tcagccccgc
     1081 gcccagccgc gtgcgtgcga gctgcagcag gccgacggcg gcctccagcg tgctcagcgt
     1141 cgcgagcgcg gtcgtcacgg cggccggctg cggatgcagc ttcagcgtgg cgccggtgat
     1201 gatgcccagc gtgccctcgc tgccgatgaa caggtcgcgc aggtcgtagc cggtgttgtc
     1261 cttgcgcagg ccgctcaagc cctcccagac ctcgccggtc gccgtcacca cctcgagccc
     1321 caggcacagc tcgcgcgcat tgccgtagcg cagcacgccg gtgccgccgg cgttggtcgc
     1381 gaggttgccg ccgatcgtgc agctgccctc ggcggccagg ctcagcgcga acagcaggcc
     1441 gtgcgcggcg gcggcctcct gcaccacttg cagcacgcag ccggcgtcga ccgtcatcgt
     1501 caggttctcg gcatcgatcg cgcgcacccg gttcatgcgc gcgaggttca gcagcacctg
     1561 ggtgccgctc gcgtcgggca ccgacgcgcc gaccaggccg gtgttgccgc cctgcgcgac
     1621 gatcgccgcg ccgtgcgcgg cgcaggtcgt cacgaccgcg gcgacctcgg ccgtcgaggc
     1681 cggccgcacg accgcgagcg cgcggccgcg gtagcgcttg cgccagtcga cttcataggc
     1741 gctgaggtct ccgtcggtca gcacgtgggc ggcaccgacc gcggcgcgca atgcctcgac
     1801 cagcgtcacg cggctggctc cttcgcgtgc cgcagccgcc agcgcacatg cgccgcgcag
     1861 gccgcgaaca gcagcaggca cagcagcagc tcgatcgcgc cgagcgcggc gccggcgccg
     1921 cggtcgctgg tcgcgcgcac cacgccttcg gtgaaataca gccacaccat caggctgagc
     1981 cagcggtagg tgtacatgcg gttgcgccac aggccgggca gcggcagcag cagcggcagc
     2041 accttcagcg ccagcgtgcg gctgccggtc ggggcgagcc acagctccca ggccaggccg
     2101 agcgcgatca gcgccagcac gctggcgagc gcgaccgcgc gcgtccaggc aatgggcgcg
     2161 ggcagggcgg cacgggcggg cagggcgatc ggtgcgtcgg tcatcgctgg ctatgatgcc
     2221 agcaatgaag ttcacgacga cgatcgagtc ggcgctgcac gagcagctgc ccgacctggt
     2281 ccagaccctg cagacctggc cctggctcga cacgctgcgc accctgcgcg cgcgctttcg
     2341 cgaagaccgg ctcggcgtga ccgcgagcag cctgacgttc acgacgctga tctcgctggt
     2401 gccgctgctg accgtgatgc tcgcggtgtt ctcggcgttc ccgatgttcg cgagcttcca
     2461 ggacgcgctg cagaagtact tcctgcagac cctggtgccg agcgacatcg ccaaccaggt
     2521 gctgcgcgcg ctgaaccagt tcgcgggcaa ggcgaagggc gtcggcagcg tcgggctggt
     2581 gctgctgttc cttgccgcgc tggcgctgat gctgaccatc gaccgcacgc tgaacggcat
     2641 ctggcgcgtg cgcaacccgc ggccgatcgc gcagcgcgtg ctggtgtact gggccgcggc
     2701 cacgctcggc ccgctggtgc tgggcgtgag cctgagcctc acttcgtatg cgctgtcggc
     2761 ctcgcaggga ctggtcggcg tgctgccggg cggcgtttcg ttgctgctcg gcacgctcga
     2821 atttctgctg ctcgttgcag ccctctccgg cctgttccac tacgtgccga acacccacgt
     2881 gcgctggcgc catgcgttcg ccggcggcgt gttcgtcgcg atcggcatcg tcggcgtgaa
     2941 gcggctgctc gcgctctact tcagcaccgt gccgacgtac agcatgatct acggcgcgtt
     3001 cgccacggtg ccgatcttcc tgatctgg
//
LOCUS       DATMJB010000505         3118 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_849099, whole genome shotgun sequence.
ACCESSION   DATMJB010000505 DATMJB010000000
VERSION     DATMJB010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849099"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1857
                     /locus_tag="VNU48_10850"
     CDS             52..1857
                     /locus_tag="VNU48_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HWI11819.1"
                     /translation="MMNFPGFSKLHGFLLAAVLVLMCQPAFAEKRVALVLGNSAYKNA
                     PPLANPTNDSAVIAATLKDAGFDVVDSRRDLPAAETRRALRDFADRARDADIAVVYYA
                     GHGIEVDGTNYLIPVDARLERDTDVFDEAFSLDRVLLAIEPAKKLRLVILDACRDNPF
                     AKSMKRTVASRAIGQGLAKVEPTSPNMLIAYAAKAGSTAADGDSKNSPFTAALSKHLT
                     TPGLDVRRAFGFVRDDVLKTTGNRQEPFVYGSLGGEDVPLVPAKVVPVPASASNPQAE
                     ARRDYELALQIGNKDAMNAFLAQYPDGFYANLAKIQINRFAAEETRVVATEKARLAEQ
                     ERARLAAEGAQKGQQVRADADAKAAEQARIAAEKAKQVAQEQAAAAEQKRVSAESAVA
                     NKAQETKAPENKAAETRVAAIPPATDNQGVNLAAQGSGPPQAEITKSVQAELRRVGCL
                     TGAADGNWNSTSQRSLTLFNRYAKTHIDASLASTDALDTIKSKSSRVCPLVCGHGYEA
                     DGDRCTRITCAAGSFLNDDNECERRREKKPVAARRGRPQTEFPEARQAPAPQRVIVTR
                     SQNGAGMGARGQPLTGREREVGCNGYGAIMSGVCP"
     gene            complement(1873..2559)
                     /locus_tag="VNU48_10855"
     CDS             complement(1873..2559)
                     /locus_tag="VNU48_10855"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HWI11820.1"
                     /translation="MPSSKKELDDFVARVKKMPAPLRLLYSRPRTFIAIAAGIAAFLL
                     MPASLRLVTRLLLGWDIFVGLYLLLVYIMVLRSGQEHIRRNAALQDDGRFVILMVTAL
                     GAFASIAAIVFELGASQRNAAYLTLATATIALTWATVHTTFALHYAHEYYGSSKGGLQ
                     FPSGDRKEEADYWDFVYFSFVIGMTAQVSDVGITDRIIRRTATVHGIISFIFNTALVA
                     LMVNIAASAI"
     gene            complement(2563..>3118)
                     /locus_tag="VNU48_10860"
     CDS             complement(2563..>3118)
                     /locus_tag="VNU48_10860"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWI11821.1"
                     /translation="AGDALLTLAFDIVTRDEIHNDATVRLLLTRALARASGIGGMVGG
                     QMLDLAGEGRFGDREPVDVARLQQMKTGALLRYGCIAGAILGQSSQKEYQALDDYGRA
                     LGEAFQIADDLLDVEGDAAALGKQTGQDAALGKTTFVTQLGVDGAKQRVRDLLARADS
                     ALSMFGAKGDVLRAAARFVAERKS"
BASE COUNT          582 a         1006 c         1039 g          491 t
ORIGIN      
        1 tgataggcag ggtgcagtgg taaagcagcg gcggctagtt caggaagtgt catgatgaat
       61 ttccctggat tctcgaaatt gcacggattt ttgctggcgg cggttctggt gctgatgtgc
      121 cagccggcct tcgccgagaa acgcgtggcg ctggtgctcg gcaactcggc ctataaaaat
      181 gccccgccgc tcgccaatcc gaccaacgac agcgccgtga tcgcggcgac gctcaaggac
      241 gccggttttg atgtcgtcga ttcccgccgc gatcttccgg cggccgaaac ccgccgcgcg
      301 ctgcgcgatt ttgccgatcg ggcccgcgac gccgacatcg ccgtggtcta ttacgccggc
      361 cacggcatcg aggtggacgg taccaactac ctgatcccgg tggatgcgcg gcttgaacgc
      421 gacaccgacg ttttcgacga ggcgttttcg ctggatcgcg tgctgctggc gatcgagccg
      481 gccaagaagc ttcgcctcgt gattctcgac gcctgccgcg acaatccctt tgccaagagc
      541 atgaagcgga cggtggcttc acgcgcgatc ggacagggcc tcgccaaggt agagccaacc
      601 agcccgaaca tgctgatcgc ctatgccgcc aaggccggct cgaccgcggc cgacggcgac
      661 agcaagaaca gtccgttcac cgcggcgttg tcgaagcatc tgacgacgcc agggctcgac
      721 gtgcgccgcg cgttcggctt cgtgcgcgac gacgtgctca agaccaccgg caaccggcag
      781 gaaccgttcg tctatggttc gctcggcggc gaggacgtgc cgctggtgcc ggcgaaggtt
      841 gtgcccgtgc ctgcctccgc ttccaatccg caggccgaag cgcgccgcga ctacgaactg
      901 gcgctgcaga tcggcaacaa ggacgccatg aacgccttcc tcgcgcaata ccccgacgga
      961 ttttacgcga acctggccaa aatccagatc aacaggttcg ccgccgagga aacccgcgtt
     1021 gtcgccaccg aaaaggcgcg gctggccgaa caggagcggg cgcggctcgc cgccgagggc
     1081 gcgcaaaagg gacagcaggt gagggctgac gccgacgcga aagccgccga gcaggcccgc
     1141 atcgccgccg aaaaagccaa gcaggtagcg caggagcagg ccgccgcggc cgagcagaag
     1201 cgcgtttcgg ctgaaagtgc cgtggccaac aaggcgcagg aaaccaaggc gccggaaaac
     1261 aaggccgccg agaccagggt tgcggcgatc ccgccggcga cagacaacca gggcgtcaac
     1321 cttgccgccc agggctccgg gccgccgcaa gccgaaatca ccaaatcggt tcaggcggag
     1381 ttgcggcgcg tcggctgcct gaccggcgcc gccgatggca actggaattc gacttcgcag
     1441 cgttcgctga cgctgttcaa tcgctacgcc aagacccata tcgacgccag tctcgccagc
     1501 accgacgcgc tcgatacgat caagagcaaa tcgtcgcggg tctgcccgct ggtctgcggg
     1561 cacggctacg aggccgatgg cgatcgttgc accaggatca cctgcgccgc cggctcgttc
     1621 ctcaacgacg acaatgagtg cgagaggcgt cgcgagaaga agccggttgc tgcccggcgc
     1681 ggcaggccgc aaacggaatt tccggaagca aggcaagctc cggcaccgca gcgcgttatt
     1741 gtcacgcgtt ctcagaatgg tgcggggatg ggagccagag gccagccgct caccggccgg
     1801 gagcgtgaag tgggttgcaa cggatacggc gccatcatgt cgggcgtgtg cccgtaacca
     1861 gggcgcgttt ggctagatcg cactcgccgc gatattgacc atcagcgcga ccagcgcggt
     1921 attgaaaatg aacgagatga tgccgtgcac ggtggcggtg cggcggatga tcctgtcggt
     1981 gatgccgacg tcggatacct gcgctgtcat tccgatcacg aacgagaaat acacgaagtc
     2041 ccaatagtcg gcttcttcct tccgatcgcc gctcgggaat tgcaggccgc ccttgctgga
     2101 gccgtaatat tcatgcgcgt agtgcagcgc gaaggtggta tggaccgtcg cccatgtcag
     2161 cgcgatcgtc gcggtcgcca gcgtgaggta ggcggcgttg cgctgcgagg ccccgagttc
     2221 gaatacgatc gcggcgatgc tggcgaaggc gccgagcgcg gtcaccatca ggatcacgaa
     2281 gcggccgtcg tcctgcagcg cggcgttgcg tcggatgtgc tcctgtccgc tccgcagcac
     2341 catgatgtag accaacagca gatatagacc gacgaaaata tcccagccga gcagcagccg
     2401 tgttaccagc cgcaacgagg ctggcatcag caagaacgcg gcgatacccg cggcgatcgc
     2461 aatgaaggtg cgcggccggc tgtagagcag gcgcagcggc gccggcattt tcttgacgcg
     2521 ggcgacgaag tcatcgagct ctttcttgct cgacggcacg tgtcagctct tgcgttcggc
     2581 gacgaagcgc gcggcggcgc gcagcacgtc gcccttggcg ccgaacatcg acagcgcgga
     2641 atcggcccga gccagaaggt cgcgcacgcg ctgcttggcg ccgtcgacgc cgagctgggt
     2701 gacgaaggtg gtcttgccga gcgccgcgtc ctgtccggtc tgcttgccga gtgccgcggc
     2761 atcgccctcg acgtcgagca ggtcgtcggc gatctggaag gcttcgccga gcgcgcggcc
     2821 gtaatcgtcg agcgcctgat attccttctg tgaggactgg ccgaggatcg cgccggcgat
     2881 gcagccatag cgcaacagcg cgccggtctt catctgctgc aatcgcgcga cgtcgaccgg
     2941 ctcgcgatcg ccgaaccggc cctcgccggc gagatcgagc atctggccgc cgaccatgcc
     3001 gccgatgccg gatgcgcgcg ccagcgcccg cgtcagcagc agacgcacgg tggcgtcgtt
     3061 gtggatctcg tcgcgggtga cgatgtcgaa cgccagcgtc aacagcgcgt cgccggcc
//
LOCUS       DATMJB010000506         4018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_854207, whole genome shotgun sequence.
ACCESSION   DATMJB010000506 DATMJB010000000
VERSION     DATMJB010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854207"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4018
                     /locus_tag="VNU48_10865"
     CDS             <1..>4018
                     /locus_tag="VNU48_10865"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HWI11822.1"
                     /translation="MKQTQQPIRLLAHSMGGLVVRAAIHKRRSVIDALMARDGARFVM
                     LGTPNQGAHSMVENLIGKGDTLRTLVRLDLKHDMQEVLDIVAGFRGALQLLPKPGFVD
                     MFQGQDGGGGRYEFQKAATWVDLKSKVTDLWFGDHHAGTPAQDVLDQASWLWAADGTA
                     TPALPAAYEAKSIYVFGVAPNTPCGVREEADGNGGTRLKMVGTTRGDGTVSWESGRIG
                     GVGHAYYLPAVHGDLPSTEEHFPALADLLTAGTTARLSVTPPALRAIQQPMPVSYDAG
                     PPTADDPDALQRGLMGGSKRNRIPPRPKRRLEVAVKAMDLRFVSQPIMVGHYERDPIA
                     GAEALIDRELLGGDLSERHSLGLYAGGRGTATVVLRVPNDLERQRGSLSGAVVTGLGS
                     YDRALSPSDLAEAVRTGALRYLLQVIDVLGKDDREVPLATLLLGYNSSANLTVAASVE
                     ALVRGVMEANAKFFETTRLNIRVSRLDIVELYLDTSISAVYALRQLGSQLAAQAARHG
                     TMLVCRNELVQGEGLRQRLFDDRSSSYWPRLIIADADRNDDACVPDVPKTKADAAAPA
                     AAPIADRLRFTYVGQRARAESVALQRQPGLIEQLVAQQLHNPSWNEDFGRMLFQLMVP
                     HDFKDAARQLDRVVLVVDTCTANLPWELMLADDPDRSDDDKLPLALRTAVVRQLTSWR
                     FRTHVRQGIERRALVVGNPSVAGFTDAFPLIDGRPTQAPPALPGAQAEAEATAVVLGS
                     MGYAVESVTGEDRSASDVLTRLYSQPWRIVHISAHGVFDLLHADGRRRSGVLLSGGLL
                     ITAAEIAAMETVPELVFLNCCHLGQMSADDKSVGRGGNKLAASVARELIEVGVRCVVV
                     AGWAVNDDSAKLFGQSFYEQVLLRRRPFGDAVFEARRAVWQAHPDDITWGAFQAYGEP
                     GWLAEPRAPDAPRADAGSPPVSPEELLDELARRRADLSRRRESFSDAEMRAQADAITA
                     LLKQRCPPAWQNLPQLQSALGRTWFELGQLERARDCLLAAVQAVDTIGLVPIHDIEKL
                     ANVESRLGEQRAHAEFAVGKHAKKGGPRTAEALIDMALRRLDGLDALVETRFATVPIA
                     GADASALLPPAPHGERAALRGSAYKRKAALQARRLLTQHLSEADKDVVRREMLDSLDR
                     AIAAYQSAEGSPGSSRFSTYHALNRLALDALTDWASPASRDAAIELAQQCRRAAAQGF
                     ALNPSLWDAITQPEALLVEHLIDGGFGRAGDAGRAVFEEVARAYADAMLNVTVKPSQI
                     DTMVSQMELLSRLCDALALLRFDDAALVRTASRLVELVQRLQPGRAARDDRPHAAPPE
                     GPKAAPGAQRKQSAKKATP"
BASE COUNT          593 a         1479 c         1431 g          515 t
ORIGIN      
        1 gatgaagcag acgcagcaac cgatccgcct gctcgcgcac agcatgggcg ggctggtcgt
       61 gcgcgccgcg atccacaagc gccgctcggt gatcgacgcg ctgatggcgc gcgacggcgc
      121 gcgcttcgtg atgctgggca cgccgaacca gggcgcgcat tcgatggtcg agaacctgat
      181 cggcaagggc gacaccttgc gcaccctggt gcgcctcgac ctgaagcacg acatgcagga
      241 ggtgctcgac atcgtcgccg gcttccgcgg cgcgctgcag ctgctgccca agcccggctt
      301 cgtcgacatg ttccagggcc aggacggcgg cggcggccgc tacgaattcc agaaggcggc
      361 gacctgggtc gacctcaaga gcaaggtcac cgacctgtgg ttcggcgacc accacgccgg
      421 cacgccggcg caggacgtgc tcgaccaggc gagctggctg tgggccgccg acggcaccgc
      481 cacgccggcg ctgcccgccg cctacgaagc gaagagcatc tacgtcttcg gcgtcgcgcc
      541 gaacacgccc tgcggcgtgc gcgaagaggc cgacggcaac ggcggcacgc gcctgaagat
      601 ggtcggcacg acgcgcggcg acggcaccgt gagctgggaa tcgggccgca tcggcggcgt
      661 cggccacgcc tactacctgc cggccgtgca cggcgacctg ccgtcgaccg aagagcattt
      721 tcccgcgctt gccgacctgc tgaccgcggg cacgaccgcg cgcctgtcgg tcacgccgcc
      781 ggcgctgcgt gcgatccagc agccgatgcc ggtcagctac gacgccggcc cgccgaccgc
      841 cgacgatccc gacgcgctgc aacgcggcct gatgggcggc tccaagcgca accgcatccc
      901 gccccgcccg aagcgccggc tcgaggtcgc ggtgaaggcg atggacctgc gcttcgtctc
      961 gcaacccatc atggtcggcc actacgagcg cgacccgatc gccggggccg aggcgctgat
     1021 cgatcgcgag ctgctcggtg gcgacctcag cgagcgccac agcctgggcc tgtatgccgg
     1081 cgggcgcggc acggcgacgg tggtgctgcg cgtgccgaac gacctcgagc gccagcgcgg
     1141 cagcctgtcg ggcgcggtcg tcaccggcct gggctcgtac gaccgcgcgc tgagcccgtc
     1201 ggacctggcc gaggcggtgc gcaccggcgc gctgcgctac ctgctccagg tgatcgacgt
     1261 gctcggcaag gacgatcgcg aggtgccgct ggccacgctg ctgctgggct acaactcgtc
     1321 ggccaacctg acggtcgcgg cctcggtcga ggcgctggtg cgcggcgtga tggaagcgaa
     1381 cgcgaagttc ttcgagacca cgcggctgaa cattcgcgtc agtcggctcg acatcgtcga
     1441 gctctacctc gacacctcga tcagcgcggt ctacgcactg cgccagctcg gctcccagct
     1501 cgccgcgcag gccgccaggc acggcacgat gctggtgtgc cgcaacgaac tcgtgcaggg
     1561 cgaggggctg cgccagcgcc tgttcgacga ccgcagttcg agctactggc cgcgcctgat
     1621 cattgccgac gccgaccgca acgacgacgc ctgcgtgccc gacgtgccga agaccaaggc
     1681 cgacgccgcc gcgccggccg cggcgccgat cgccgaccgg ctgcgcttca cctacgtcgg
     1741 ccagcgcgcg cgtgccgagt cggtcgcgct gcagcgccag ccggggctga tcgagcagct
     1801 cgtcgcgcag cagctgcaca acccgagctg gaacgaggac ttcggccgca tgctgttcca
     1861 gctgatggtg ccgcacgact tcaaggacgc cgcgcgccag ctcgaccgcg tcgtgctcgt
     1921 cgtcgacacc tgcaccgcga acctgccctg ggagctgatg ctggccgacg accccgaccg
     1981 cagcgacgac gacaagctgc cgctggcgct gcgcaccgcg gtcgtgcggc agctgacgag
     2041 ctggcgcttc cgcacccacg tgcgccaggg catcgagcgg cgtgcgctgg tggtcggcaa
     2101 cccgtcggtg gccggcttca ccgacgcgtt cccgctgatc gacggccggc cgacgcaggc
     2161 gccgccggcg ctgcccggcg cgcaggccga ggccgaggcg accgcggtcg tgctcggcag
     2221 catgggctac gccgtcgagt cggtcacggg cgaggaccgc agcgccagcg acgtgctgac
     2281 caggctgtac agccagccct ggcgcatcgt gcacatctcg gcgcatggcg tgttcgacct
     2341 gctgcacgcc gacggccggc gccgcagcgg tgtgctgctg tcgggtggcc tgctgatcac
     2401 cgcggccgag atcgcggcga tggaaacggt gcccgagctg gtgttcctga actgctgcca
     2461 cctcggccag atgagtgcag acgacaagag cgtgggcagg ggcggcaaca agctcgcggc
     2521 cagcgtcgca cgcgagttga tcgaggtcgg cgtgcgctgc gtcgtcgtgg ccggctgggc
     2581 cgtcaacgac gattccgcga agctgttcgg ccagtccttc tacgagcagg tgctgctgcg
     2641 ccgccgaccc ttcggcgacg cggtgttcga ggcgcgccgc gcggtctggc aggcccaccc
     2701 cgacgacatc acctggggcg ccttccaggc ctacggcgag cccggctggc tggccgaacc
     2761 gcgcgcgccc gacgcgccgc gtgccgacgc cggcagcccg ccggtgtcgc ccgaggaact
     2821 gctcgacgag ctcgcgcgcc gccgcgccga cctgtcgcgc cggcgcgaga gtttttccga
     2881 cgccgagatg cgcgcccagg ccgacgcgat cacggccctg ctgaagcagc gctgcccgcc
     2941 ggcgtggcaa aacctgccgc agctgcagtc ggcactcggc cgcacctggt tcgagctcgg
     3001 ccagctcgaa cgcgcgcgcg attgcctgct cgccgcggtg caggccgtcg acacgatcgg
     3061 cctggtgccg atccatgaca tcgagaagct cgcgaacgtc gaatcgcgcc tcggcgagca
     3121 gcgcgcgcac gccgagttcg cggtcggcaa gcatgcgaaa aagggcggtc cgcgcacggc
     3181 cgaggcgttg atcgacatgg cgctgcggcg tctcgacggc ctcgacgcgc tggtcgaaac
     3241 gcgcttcgcc acggtgccca tcgccggcgc cgacgccagc gccttgctgc cgccagcgcc
     3301 gcacggcgag cgcgccgcgc tgcgcggcag cgcctacaag cgcaaggcgg cgctgcaggc
     3361 gcggcgcctg ctgacgcagc acctgagcga ggccgacaag gacgtcgttc ggcgcgagat
     3421 gctcgacagc ctcgaccgcg cgatcgccgc ctaccagagc gccgaaggca gcccggggag
     3481 cagccgcttc agcacgtacc acgcgctgaa ccgcctcgcg ctcgacgcgc tgaccgattg
     3541 ggccagcccg gcgagccgcg acgccgcgat cgaactcgcg cagcagtgcc ggcgcgccgc
     3601 ggcgcagggc ttcgcgctca acccgagcct gtgggacgcg atcacgcagc ccgaggcctt
     3661 gctcgtcgag cacctgatcg acggcggctt cggccgcgcc ggtgacgccg ggcgtgcggt
     3721 gttcgaggag gtcgcgcgcg cctatgccga tgcgatgctc aacgtcaccg tgaagccgtc
     3781 gcagatcgac acgatggtgt cgcagatgga actgctgtcg cgtctgtgcg atgcgctcgc
     3841 gctgctgcgc ttcgacgacg cggcgctggt gcgcaccgcc agccgcctgg tcgaactggt
     3901 gcagcgcctg cagcccggcc gcgcggcacg cgacgatcga ccgcacgcgg cgccgcccga
     3961 ggggccgaag gccgcgcccg gtgcgcagcg caaacagtcg gcgaagaaag ccacgcca
//
LOCUS       DATMJB010000507         4481 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_369520, whole genome shotgun sequence.
ACCESSION   DATMJB010000507 DATMJB010000000
VERSION     DATMJB010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369520"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /locus_tag="VNU48_10870"
     CDS             complement(<1..1339)
                     /locus_tag="VNU48_10870"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWI11823.1"
                     /translation="MTILARRAFFIGSTSCLLLYAASNSAMAQDAAPAAASTQLPDIT
                     VSAPSPIVRRKLTPTRTTVRTARAVAGRNREPAPQPAEVAAAPQQGVLPIVTDQFATV
                     SVVPNEEIRRSGAATLGDLLFSKPGITGSEFAPGASSRPIIRGLDVNRVGIVENGVGS
                     NGASDLGEDHFVPIDPVATNQVEVIRGPATLRYGSQSIGGVVSATNNRIPETLPACAA
                     PFQTYGLPAKAPVTSTAQAGCMTAETRTAASSVDRGVEGGILLDAGGGDFAIHADAYG
                     RKSGDYNIPGYPYRFDQTRPVSGRQPNSAAQADGASIGGSYIFHGGFIGAAITQNDAL
                     YRIPGIDGADHRTRIDAHQTKITAKGEYRPDAAAIEAVRFWAGATDYRHNEIGLATIG
                     DPSTDGVRQTFTNKEQEGRVEVQMMPFNARFASVTTAFGLQASHQELTAPSPDG"
     gene            complement(1469..1897)
                     /locus_tag="VNU48_10875"
     CDS             complement(1469..1897)
                     /locus_tag="VNU48_10875"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HWI11824.1"
                     /translation="MKWFRSHIKQGSLTALLALAIQFALSFGHFHGVAAQAAPAFQSG
                     LELDLSQADVLGAPNVIVDPDQQQSPPAHDTDPQNEPPQNGACAICAVISLAGSLLFA
                     TPPLLLLPQSIELLHLIADAEFVHLNNVRADFQPRAPPLS"
     gene            complement(2050..4113)
                     /locus_tag="VNU48_10880"
     CDS             complement(2050..4113)
                     /locus_tag="VNU48_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11825.1"
                     /translation="MNYGVAFTPLVPSLVLWIGLAAVVVIAALLLLGRARGAAVRVTA
                     LALILLALANPSFTREDREPLSSVAAVVIDKSPSQNFGARTAETARAQEALVDSLKKV
                     KGLEVRVVEAGHADGETDGTKLFGALSSALSDVPVDRVAGAFLITDGRVHDIPANAAA
                     LGFQAPVHALVTGRKDERDRRIAISAAPHFGIVGQVQTITYRLDDQGVTGERAKIVIR
                     RDGEVISERSLQSGQTASVEIDIKHAGPNIVEIEASPLENELTLVNNRAVVAIDGVRD
                     KLRVLLVSGEPHSGERTWRNLLKSDASVDLVHFTILRPPEKQDGTPINELSLIAFPTR
                     ELFQQKINEFQLIIFDRYARQGVLPIAYFDNIARYVRAGGAVLVSAGPDYASTTSIWR
                     TPLDSVLPAEPVGVNEKPYYAHLSDAGKRHPVTRGLEGSGAEPPRWSRFFRTVETRNA
                     IVPPVMTGADGKPLLLLSRFGEGRVALLLSDHIWLWARGYEGGGPHLDLLRRMSHWLM
                     KQPDLDEEALRLQVQGHDLVVLRQTMADSVSPVTVTSPGGATRELTLSASEPGTWRTT
                     TPANELGLWQATDGALKALISVGPTNPKEFSEVTSTIDLLKPLAQATGGDARRVVDGG
                     SIDLPRIVPVRASSIFRGDGWMGVKMRDASVVRGVGVLPMFTGLLGLLLLLGAFAATW
                     LREGR"
     gene            complement(4178..>4481)
                     /locus_tag="VNU48_10885"
     CDS             complement(4178..>4481)
                     /locus_tag="VNU48_10885"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HWI11826.1"
                     /translation="RFNSGPTWVETLPRDDGDDAASRPARGGDGVSPIIITSNDLAGA
                     WALRPDGQPMLPLTPGEPRQREFAFRAGVNIVMYTLTGNYKADQVHAPALIERLGQ"
BASE COUNT          763 a         1451 c         1505 g          762 t
ORIGIN      
        1 ggccgtccgg actcggcgcg gttagttcct ggtggctcgc ctgcaggccg aacgccgtcg
       61 tcaccgaggc aaagcgtgcg ttgaacggca tcatctgtac ttcgacgcgg ccttcctgct
      121 ccttgttggt gaaggtctgc ctcacgccgt cggtcgaggg gtcgccgatg gtggcaagac
      181 cgatctcgtt gtggcggtag tcggtcgcac cggcccagaa cctgacggcc tcgatcgccg
      241 ccgcatcggg ccgatactcg cccttggcgg tgatcttggt ctgatgcgcg tcgatgcggg
      301 ttcggtgatc ggcgccgtcg atgccgggaa tacggtagag cgcgtcgttc tgcgtgatcg
      361 ccgcaccgat gaagccgccg tggaagatgt aggagccgcc gatcgaggcg ccgtcggctt
      421 gtgccgccga attcggctga cggccgctga ccggcctcgt ctggtcgaat cggtagggat
      481 agccgggaat gttgtaatcg ccggatttgc gcccatacgc atcggcatgg atggcgaagt
      541 cgccgccgcc ggcatccagc aaaatgccgc cttcgacgcc gcggtcgacg gaactcgccg
      601 cggtgcgggt ttccgccgtc atgcagccgg cttgcgccgt cgatgtcacg ggcgccttgg
      661 ccggcaggcc ataggtctga aacggcgccg cgcaggcggg cagggtttcc gggatccggt
      721 tgttggtcgc gctgaccacg ccgccgatcg attgcgaccc gtaacgcaac gtcgccggac
      781 cgcggatcac ctccacctga ttggtcgcaa ccggatcgat cggaacgaag tgatcctcgc
      841 cgagatcgga cgcgccgttg ctgccgacgc cgttctcgac tatcccgaca cggttgacgt
      901 caagccccct gatgatcggc cggctcgatg cgcccggcgc gaattccgaa ccggtgattc
      961 cgggcttgga aaacagcagg tcgcccaagg tcgctgcgcc ggagcggcgg atttcctcgt
     1021 tcggcaccac gctgaccgtg gcgaattgat cggtcacgat cggaagcacg ccctgttgcg
     1081 gcgctgccgc tacttctgcg ggttgtggcg ccggttcgcg gttgcggccg gcaaccgcgc
     1141 gtgcggtgcg cacggtcgtg cgcgtcggcg tcagttttcg ccgcacgatc ggcgacggcg
     1201 cggacaccgt gatgtcggga agctgcgtgg aggcggctgc gggcgcggcg tcctgcgcca
     1261 tcgcactgtt gcttgcagca tatagcagca ggcaactcgt gctgccgatg aagaacgcac
     1321 gtcttgcgag aatggtcatt tccgtatcct gaacaaagcc gccgccggat tcgtggtggg
     1381 cgaactggag aggccaccga tttcgattca tccttcggat cggccgcatg atgcggcgaa
     1441 tcctccagcc gtgatcaatc aatcgatgtc aggagagggg aggagcgcga ggctggaaat
     1501 cggcgcggac attgttcaga tgaacgaatt cggcgtcggc gatcaggtgc agcaattcaa
     1561 tcgactgcgg cagcagcaac agcggcgggg tagcaaacag cagcgatccc gccagcgaga
     1621 taacggcgca gattgcgcaa gccccattct gtggaggctc attctgtggg tcggtatcgt
     1681 gagcgggcgg agattgctgc tggtcgggat cgacgattac gttcggcgca ccgagcacgt
     1741 cggcttgcga aaggtccagc tcaaggcccg actgaaaggc aggcgcggcc tgcgcggcga
     1801 ccccatggaa atgcccgaac gacagcgcaa actggatcgc cagcgccaaa agcgctgtca
     1861 gcgagccctg tttgatgtgc gaacggaacc acttcatgcg gatagtgccc cacatcggcg
     1921 gccaaggcgt ccccgccagt cccgatgtta taatgtaaca tcgcgcgctt gagctatcgg
     1981 tgtcaaccgt agcccggcga cagcaaactc aattcggagg gactgtgtga ttggtgcaac
     2041 gaagcggcgt caacgccctt cgcgcagcca ggtcgcggcg aaggcgccga gcagcaacaa
     2101 caaaccgagc aggccggtga acatcggcag cacgccgacg ccgcggacca cgctggcatc
     2161 gcgcatcttc acgcccatcc agccgtcacc gcggaatatg ctggaggccc gcaccggcac
     2221 gatgcgcgga aggtcgatac tgccgccatc caccacgcgc cgcgcgtcgc cgccggtggc
     2281 ctgcgccagc ggtttgagca ggtcgatggt ggatgtcact tccgaaaact ctttcggatt
     2341 ggtcgggccg acgctgatca gcgccttcag cgccccgtcg gtggcctgcc acagcccgag
     2401 ctcgttggcg ggagtggtgg tccgccaagt cccgggctcg ctggcgctca gcgtcagttc
     2461 gcgggtggcg ccgcccggcg aggtcaccgt caccggcgat acgctgtcgg ccatggtctg
     2521 gcgcagcacc acgagatcgt ggccctgcac ctgcaggcgc agcgcttcct cgtccagatc
     2581 gggctgcttc atcagccagt gcgacatccg ccgcagcaga tcgagatgcg ggccgccgcc
     2641 ttcatagccg cgcgcccaaa gccagatatg atccgacagc aacagcgcga cgcggccttc
     2701 gccgaaccgc gacagcagca acagcggctt gccgtcggcg ccggtcatga cgggcggcac
     2761 gatcgcgttc cgggtttcga cggtgcggaa gaaccggctc cagcgcggcg gctcggcgcc
     2821 cgagccttcc agcccgcgcg tcaccggatg gcgtttgccg gcgtcgctca gatgcgcgta
     2881 atacggcttc tcgttgacgc caacgggttc ggccggcagc accgaatcca gcggcgtgcg
     2941 ccagatactg gtggtggagg catagtcggg accggccgac accagcaccg cgccgccggc
     3001 gcgcacatag cgcgcgatgt tgtcgaaata ggcgatcggc agcacgcctt gccgggcgta
     3061 gcggtcgaag atgatcagct ggaattcgtt gatcttctgc tggaacagct cgcgcgtcgg
     3121 aaacgcgatc agcgacaatt cattgatcgg ggtgccgtcc tgcttctccg gcgggcgcag
     3181 aatcgtaaag tgcacgagat cgacgctggc atcggatttc agcaggttgc gccaggtgcg
     3241 ctcgccggaa tgcggctcgc cggaaaccag cagcacccgc aatttgtcgc gtacgccgtc
     3301 gatcgcgacc acggcgcggt tgttgaccag cgtcagttcg ttctcgaggg gcgaagcctc
     3361 gatctcgacg atgttcggcc cggcatgctt gatgtcgatc tcgacactgg cggtctggcc
     3421 gctttgcaag ctgcgctcgc tgatcacttc gccatcgcgc cggatcacga tcttggcgcg
     3481 ctcgccggtg acgccctgat cgtcgagccg gtaggtgatg gtctggacct gcccgacgat
     3541 gccgaaatgc ggtgcggccg aaatcgcgat gcggcggtcg cgctcgtcct tgcggccggt
     3601 gaccagcgcg tgcaccgggg cctggaatcc gagcgcagcg gcattggccg gaatatcgtg
     3661 cacccgcccg tcggtgatca ggaaggcgcc ggcgacgcga tcgaccggaa cgtccgagag
     3721 cgcggatgac agcgcgccga acagtttggt gccgtcggtt tcaccgtcgg catggccggc
     3781 ctcgaccacc cgcacttcca gccccttgac cttcttcaag ctgtcgacca gcgcctcctg
     3841 cgccctggcg gtctcggcgg tccgcgcgcc gaaattctga ctcgggctct tgtcgatcac
     3901 gacggcggcg accgatgaca gcggctcgcg atcctcgcgg gtgaaggacg gattggccag
     3961 cgccagcaga atcagcgcca acgccgtgac ccgcaccgcc gctccgcgcg cccgccccaa
     4021 cagcagcaac gccgcgatca cgacgacggc ggcgagcccg atccagagca cgagcgaggg
     4081 gacaagaggc gtaaaggcga cgccgtaatt catgacccgt ccttaaactg cagcatgatc
     4141 tggtcggaaa accggttccc acttttccgg agcatgttca ttgccccagc cgttcgatca
     4201 gcgcgggtgc gtgcacctgg tcggccttgt agtttccggt cagcgtgtac atcacgatgt
     4261 tgacgccggc gcggaacgcg aattcgcgct gccgcggctc gcccggcgtc agaggcagca
     4321 tcggctggcc gtccggacgc agcgcccagg cgccggcaag atcgttcgag gtgatgatga
     4381 tcggcgacac gccgtcacca ccgcgggcgg gacgcgaggc agcgtcatcg ccgtcgtcgc
     4441 gcggcagcgt ctcgacccag gtcggtccgg aattgaagcg g
//
LOCUS       DATMJB010000508         9346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_849137, whole genome shotgun sequence.
ACCESSION   DATMJB010000508 DATMJB010000000
VERSION     DATMJB010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9346
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_849137"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VNU48_10890"
     CDS             complement(<1..405)
                     /locus_tag="VNU48_10890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI11827.1"
                     /translation="MRRRDAIKWLAGSSAALALPALAQTTNRIVLGQSAAFSGPAAQL
                     GIQMNIGARIYFNALNAQGGVNGQGIELRTLDDGYEPDRCKANTEKFLNDEVFGLFGY
                     VGTPTSLAALPLVVDAKIPFFGPFTGAQALREP"
     gene            570..2627
                     /locus_tag="VNU48_10895"
     CDS             570..2627
                     /locus_tag="VNU48_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HWI11828.1"
                     /translation="MTTTTVRAACPHDCPDTCAIRVTVQDGRAIKVQGDPDHPPTHGA
                     LCTKVSRYPERTYHPERVLHPLKRSGPKGSGQFTRVSWDEALTDIAARLRAIAARDPQ
                     AIVPYSYAGTMGLVQGESMAARFFNKLGASLLDRTICSSAGGEALAATYGGKVGMHLE
                     FYAESRLILIWGSNSIASNLHFWTFAQAAKRAGAKLVCIDPRKTETADKCHQHIAILP
                     GTDGALALGLMHELIVNGWLDEDYIARHVDAAGWPLLRARALEWTPERVAQVCGISAD
                     EVRGLGADYGTTRPAAIRLNYGMQRARGGGNAVRLIALLPGLVGAWRQRGGGLLLSSS
                     GWFKSVRDDAALQRPELLAGRRPRTINMSTIGDDLLRHSSPGFGPKIEALVVYNSNPV
                     AVAPASPKVVQGFAREDLFTVVLEHFMTDTADHADYVLPATTQLEHLDVHTSYGHTYA
                     MINEAAVAPLGEARPNTQIFRELAARMGFAEACFTDDDEALARAAFRRDRVDFDALRA
                     QGWVKLPIADAPFAEGGFPSADGRCIIDSPEFGVPDFVANYESAASAPELARRFPLAM
                     ISPPARNFLNSSFVNVRSLRDIEGEPLVELHALDARGRGIASGQMVRVFNERGSYLCR
                     ARISERARPGVVNGLGVWWRKLGAAGTNVNELTHQRLTDIGRAPSFYDCLVEVRPADA
                     AVS"
     gene            2660..3712
                     /locus_tag="VNU48_10900"
     CDS             2660..3712
                     /locus_tag="VNU48_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWI11829.1"
                     /translation="MGAVGVALFAAATVCVSSGCSTIGYYAQSVGGHLAIVRAARPVP
                     DWLADEHTSAALKARLELSQRIRDFAVSELHEPDNASYRRYADLKRAAAVWNVVAAPE
                     LSLKLQTWCFAVVGCVGYRGYYDQAGANAFADGLRAQGLDVSVYGVPAYSTLGLLPGD
                     TLKDPLLNTFIDYPEGELARLIFHELAHQIAYAKGDTVFNESFATTVERLGAERWLRE
                     RAGPPARAEYEQVEARRRDFRALTTRYRDRFAALYDSAASDDDKRRAKAALLAELRAD
                     YEAMKRERWGGFSGYDGWFARANNASFGVLAAYNQLVPNFERLFEREGRDFTRFYAEV
                     RRVAALPPAERRDALP"
     gene            3725..4066
                     /locus_tag="VNU48_10905"
     CDS             3725..4066
                     /locus_tag="VNU48_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HWI11830.1"
                     /translation="MPDIHIRRDHALGLAKARKLALQWAEDAEARFDMECTILEGETS
                     DTVEFTRAGVRGELVVAPDHFVLDAKLGFLLGAFSRTIESEIEKNLDALLAKSSAPAK
                     ARPAAKKAAKK"
     gene            4074..5513
                     /locus_tag="VNU48_10910"
     CDS             4074..5513
                     /locus_tag="VNU48_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HWI11831.1"
                     /translation="MQTSPERSSQRMSRVDTAWLRMDNDVNLMMIVGVWLLRPGITHA
                     QLCERVEDKLLKYERFRQKVVPDAMGAMWVPDTAFDVKRHVLHQKLQRARGQSAREAL
                     QALVGELANTPLAPDRPLWQFHLIDGYEGGSALVARVHHCIGDGIALISVMLAITDDG
                     IDPPRRRPRAPPGSDGDWLSDAVMKPLTDLASKAIGVCDGGVGKSMEMLSNPQQGLLG
                     GIEMLRLGAKVLGDVGAMALMADDSPTLLKGKPLGQKVVAWSEPLPLDQVKAIGKALN
                     CSINDVLLACVAGAIGAYLREQGDDPTGKEIRAMVPVNLRPIEQAWKLGNRFGLAPLT
                     LPIGIDNPVERVYAVRVRMNQLKDSYQPLLAFAVLAVAGALIKPVQDGLLGLFAKKAT
                     AVMTNVPGPATPLKFCGATLRQTMFWVPASGDIGVGVSILSYGGGVQFGLITDRHLCP
                     HPQRIIDRFEPEFEKLLLLAMMLPWGLRP"
     gene            complement(5532..5726)
                     /locus_tag="VNU48_10915"
     CDS             complement(5532..5726)
                     /locus_tag="VNU48_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11832.1"
                     /translation="MGVTDKIWGALTSMIKLEDKVMRQAEAMKAQQARIEDLTARVIR
                     LEAHLELLTGAALVKRLKGS"
     gene            complement(5726..6025)
                     /locus_tag="VNU48_10920"
     CDS             complement(5726..6025)
                     /locus_tag="VNU48_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11833.1"
                     /translation="MAAAVARIVVQATAQDKRAIAAKAKKLDIPISELMRRGAFSYES
                     SESDAELAALADAAKNAADRAGAAIDDAIDFIAASNGRIAAMEAKATKRPTRNVA"
     gene            complement(6192..6965)
                     /locus_tag="VNU48_10925"
     CDS             complement(6192..6965)
                     /locus_tag="VNU48_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11834.1"
                     /translation="MAKWTAFPHDAADYSYDTAALQRHWARLHAGDAEPLPADAKVLA
                     AWALFHAGEFQEAVEAGMKAGGAGITAANKAQAIYANYLEKSEKAKLALFMEVAERAE
                     AQLATDPKNANAYYFMAYALGRYSQGISIAKALAQGLGSKVKSALEAAIKLAPKHADA
                     HIALGAFHAEVIDKVGSLLGRTQGASKDAGLTMYKTALKLNPTSAIAMVEYANGLVML
                     EGDKKMKEATQLYEDAAACVPMDAMERLDVEMAKAELED"
     gene            complement(6969..7637)
                     /locus_tag="VNU48_10930"
     CDS             complement(6969..7637)
                     /locus_tag="VNU48_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWI11835.1"
                     /translation="MATQTKKAPRRTAERILEVTLALFNRFGEPNVSTTLISAELGIS
                     PGNLYYHYPAKDELINSLFDRYERALAELLRAADDVRNVEDAWLFFHMLFELIWQYRF
                     LYRDLNDLLSKNRRLETHFQFVLKNKGKAVQNVLDGLARSNAMRMDSADIEPVATAMV
                     VVLTYWLSYEYVRDPRNALEPESAGAALLRGAFHVLSLLSPYLEPDQREHLHGLVGNY
                     NPGD"
     gene            7722..8657
                     /locus_tag="VNU48_10935"
     CDS             7722..8657
                     /locus_tag="VNU48_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI11836.1"
                     /translation="MPAISFQHVGKTYISARGATQALDDVSFDIEPGEFFGLLGPNGA
                     GKTTLISILAGLARATSGTVKVLGHDVVADYAQARRSLGLVPQELVFDPFFSVREALR
                     IQSGYFGVRKNDAWIDELLANLGLADKADANMRQLSGGMKRRVLVAQALVHRPPVIVL
                     DEPTAGVDVELRQTLWHFVARLNKEGHTVLLTTHYLEEAEALCGRLAMLKQGRVVALD
                     RTSALLAGTASTMLRFKMDAPLPASLAAQSRVTGRIVQITAHDAREVETILGVLRNAG
                     CQPEDLEIGRADLEDVFLEIMSGTPRAAPPLGVAA"
     gene            8654..>9346
                     /locus_tag="VNU48_10940"
     CDS             8654..>9346
                     /locus_tag="VNU48_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI11837.1"
                     /translation="MTPVHVLSGADTLLYKEVLRFWKVAFQTVAAPVLTAILYLLIFG
                     HVLEDRVKVFGSVGYTSFLIPGLVMMSVLQNAFANSSSSLIQSKITGNLVFLLVAPLS
                     HWAWFVAYVGASVVRGAVVGAGVMIATVWFAPLHLAQPGWIVVFGVAGAALMGALGLM
                     AGLWAEKFDQIAAFQNFIIMPMTFLSGVFYSVGSMPPFWSGVSHLNPFFYMIDGFRHG
                     FFGVSDVSPWTGL"
BASE COUNT         1398 a         3175 c         3250 g         1523 t
ORIGIN      
        1 gggctcgcgc agcgcctgcg cgccggtgaa cggcccgaag aacgggatct tcgcgtcgac
       61 caccagcggc agcgccgcca gcgaggtcgg cgtgccgacg tagccgaaca ggccgaacac
      121 ctcgtcgttc aggaacttct cggtgttggc cttgcagcga tcgggctcgt agccgtcgtc
      181 cagcgtgcgc aactcgatgc cctggccgtt cacgccgcct tgggcgttca gcgcgttgaa
      241 gtagatgcgc gccccgatgt tcatctggat gcccagctgc gcggccggcc cgctgaacgc
      301 ggccgactgc ccgagcacga tgcggttggt ggtctgcgcc agtgccggca gcgccagcgc
      361 ggcgctcgaa ccggcaagcc acttgatggc gtcacgacgt ctcatggaat cctcaacgat
      421 ggattgcggg gcaaccgcgg cgctctgcgc cggggccctt ctggtgtttt cacagggtgt
      481 cgcagcatag gcgctgacag gcggcgcgtc atgccggtgc gggcgccctg cacggtcatg
      541 tgggcgggcg gtgtgcaata gttcacccca tgaccacgac gaccgtacgg gccgcctgcc
      601 cgcacgactg cccggacacc tgcgcgatcc gcgtcacggt gcaggacggc cgggcgatca
      661 aggtgcaggg cgaccccgac cacccgccga cgcacggcgc gctgtgcacc aaggtctcgc
      721 gttaccccga gcgcacgtat caccccgagc gcgtgctgca tccgctgaag cgcagcgggc
      781 cgaaggggag tggacagttc acgcgcgtga gttgggacga ggccttgacc gacatcgccg
      841 cccggctgcg cgcgatcgcc gcgcgtgacc cgcaggcgat cgttccctac agctacgccg
      901 gcacgatggg attggtccag ggcgagagca tggcggcgcg cttcttcaac aagctcggcg
      961 cctcgctgct cgaccgcacg atctgctcca gcgccggcgg cgaggcactg gccgcgacct
     1021 acggcggcaa ggtcggcatg cacctcgagt tctacgccga gagccggctg atcctgatct
     1081 ggggcagcaa ctcgatcgcg tcgaatctcc acttctggac cttcgcgcag gccgcgaagc
     1141 gcgccggcgc gaagctcgtc tgcatcgacc cgcgcaagac cgagaccgcc gacaaatgcc
     1201 accagcacat cgcgatcctg ccgggcaccg acggcgcgct cgcactgggg ctgatgcatg
     1261 agctgatcgt gaacggctgg ctcgatgaag actacatcgc ccgccacgtc gatgccgccg
     1321 gctggccgct gctgcgtgca cgcgcgctcg aatggacgcc ggagcgcgtc gcgcaggtct
     1381 gcggcatcag cgccgacgag gtgcgcggct tgggcgccga ctacggcacg acgcggcccg
     1441 ccgcgatccg cctgaactac ggcatgcagc gcgcgcgcgg tggcggcaac gcggtgcgcc
     1501 tgatcgcgct gctgcccggc ctggtcggcg catggcgcca gcgcggcggc gggctgctgc
     1561 tgtcgagctc gggctggttc aagagcgtgc gcgacgatgc cgcgctgcag cggcccgagc
     1621 tgctcgccgg ccgccggccg cgcacgatca acatgagcac gattggcgac gacctgttgc
     1681 gccattcgtc gcctgggttc ggcccgaaga tcgaggcact ggtcgtctac aacagcaacc
     1741 cggtcgcggt cgcgcccgcg tcgcccaagg tggtgcaagg cttcgcgcgc gaagacctgt
     1801 tcaccgtcgt gctcgagcac ttcatgaccg acaccgccga ccacgccgac tacgtgctgc
     1861 ccgccaccac gcagctcgag cacctggacg tgcacaccag ctacggccac acttacgcga
     1921 tgatcaacga ggccgcggtc gcgccgctgg gcgaggccag gccgaacacg cagatcttcc
     1981 gcgagctggc ggcgcggatg ggcttcgccg aggcctgttt caccgacgac gacgaggcgc
     2041 tggcgcgcgc cgcgttcagg cgcgatcgcg tcgatttcga tgcgctgcgc gcgcagggct
     2101 gggtcaagct gccgatcgcc gacgcgccgt tcgccgaagg cggctttccg agcgccgacg
     2161 gccgctgcat catcgactcg cccgagttcg gcgtgcccga cttcgtcgcg aactacgaat
     2221 cggccgccag cgcgcccgag ctcgcgcggc gcttcccgct ggcgatgatc tcgccgccgg
     2281 cacgcaactt cctgaactcg agcttcgtca acgtcaggag cctgcgcgac atcgagggcg
     2341 agccgctggt cgagctgcac gcgctcgatg cgcgcggccg cggcatcgcc agcggccaga
     2401 tggtgcgcgt cttcaacgag cgcggcagct acctgtgccg cgcgcgcatc agcgagcggg
     2461 cacgccccgg cgtcgtcaac gggctgggcg tgtggtggcg caagctgggc gcggcgggca
     2521 ccaacgtcaa cgagctgacg caccagcggc tcaccgacat cggccgcgca cccagcttct
     2581 acgactgcct ggttgaggtg cgaccggccg acgccgccgt gtcgtgaccc gtggctggca
     2641 ggcgttgagc gtggcgggtc tgggtgcggt cggcgttgcg ttgttcgccg ccgccaccgt
     2701 ctgcgtgagc tcgggctgca gcacgatcgg ctattacgcg cagtcggtcg gcggccactt
     2761 ggcgatcgtg cgcgcggcgc ggccggtgcc cgactggctc gccgacgagc acacgagcgc
     2821 cgcgctgaag gcgcgcctcg agctcagcca gcgcatccgc gatttcgcgg tcagcgaact
     2881 gcacgagccc gacaacgcga gctaccggcg ctatgccgac ctgaagcgtg cggccgcggt
     2941 ctggaacgtg gtcgccgcgc ccgagctcag cctgaagctg cagacttggt gctttgcggt
     3001 ggtcggctgc gtcggctacc gcggctacta cgaccaggcc ggggcgaacg ccttcgccga
     3061 cggcctgcgc gcacaggggc tggacgtgag cgtctacggc gtgccggcgt actcgaccct
     3121 cggcctgctg cccggcgaca cgctcaagga cccgctgctg aacaccttca tcgactaccc
     3181 ggaaggcgag ctggcgcggc tgatcttcca cgagctcgcg caccagatcg cctatgcgaa
     3241 gggcgacacc gtgttcaacg aatcgttcgc gaccacggtc gagcgcctcg gcgccgagcg
     3301 ctggctgcgc gagcgcgccg ggccgccggc gcgtgccgag tacgaacagg tcgaggcacg
     3361 tcgacgcgac ttccgtgcgc tgacgacgcg ctaccgcgat cgcttcgccg cgctgtacga
     3421 cagcgccgcg agcgacgacg acaagcgccg cgccaaggcc gcgctgctgg ccgagttgcg
     3481 ggccgactac gaggcgatga agcgcgagcg ctggggcggg ttttccgggt atgacggctg
     3541 gttcgcccgc gccaacaatg cgtcgttcgg cgtgctggcg gcctacaacc agctggtgcc
     3601 gaatttcgag cgtctgttcg agcgcgaagg ccgcgacttt acgcgcttct atgcagaggt
     3661 caggcgagtc gcagcgctgc caccggccga gcggcgcgac gcactgccct aaggagaatc
     3721 aagagtgccc gacatccaca tccgccgcga ccacgcgctc ggcctcgcca aggcgcgcaa
     3781 gctcgcgctg caatgggccg aggatgccga ggcccggttc gacatggagt gcacgatcct
     3841 cgaaggcgag accagcgaca cggtcgaatt cacgcgcgcc ggcgtcaggg gcgagctggt
     3901 cgtcgcgccg gaccacttcg tgctcgacgc gaagctcggc ttcctgctcg gtgcgttcag
     3961 caggaccatc gagtcggaga tcgagaagaa cctcgatgcg ctgctcgcca agagcagcgc
     4021 gcccgcgaag gccaggcccg ccgcgaagaa ggcggcgaag aagtagcgac gcgatgcaga
     4081 cttcgccaga gcgcagctcg cagcgcatgt cgcgcgtcga caccgcgtgg ctgcggatgg
     4141 acaacgacgt gaacctgatg atgatcgtcg gcgtctggct gctgcggccc ggcatcacgc
     4201 acgcgcagct ctgcgaacgg gtcgaggaca agctgctgaa gtacgagcgc tttcgccaaa
     4261 aggtggtgcc cgatgcgatg ggcgcgatgt gggtgcccga tacggcgttc gacgtgaagc
     4321 gccacgtgct gcaccagaag ttgcagcgcg cgcgcggcca gagcgcgcgc gaggcgctgc
     4381 aggcgctggt cggcgaactc gcgaacacgc cgctggcccc cgaccgcccg ctgtggcagt
     4441 tccacctgat cgacggctac gaaggcggca gcgcgctggt ggcgcgcgtg caccactgca
     4501 tcggcgacgg catcgcgctg atctcggtga tgctggcaat caccgacgac ggcatcgacc
     4561 cgcccaggcg caggccgcgg gcgccgcccg gcagcgatgg cgactggctc tccgatgccg
     4621 tgatgaagcc gctgaccgat ctggcgagca aggcgatcgg cgtgtgcgac ggcggtgtcg
     4681 gcaagagcat ggagatgctg tcgaacccgc agcagggcct gctcggcggc atcgagatgc
     4741 tgcgcctggg cgccaaggtg ctgggcgacg tcggcgcgat ggcgctgatg gccgacgact
     4801 cgccgaccct gctgaagggc aagccgctcg gccagaaggt cgtcgcctgg agcgagccct
     4861 tgccgctgga ccaggtcaag gcgatcggca aggcgctgaa ctgctcgatc aacgacgtgc
     4921 tgctcgcctg cgtggccggc gcgatcggcg cgtacctgcg cgagcagggc gacgacccaa
     4981 cgggcaagga gatccgcgcg atggtgccgg tgaacctgcg cccgatcgag caggcgtgga
     5041 agctcggcaa ccgcttcggc ctggcaccgc tgaccttgcc gatcgggatc gacaatcccg
     5101 tcgagcgcgt ctacgcggtg cgcgtgcgca tgaaccagct gaaggacagc taccagccgc
     5161 tgctcgcgtt cgcggtgctc gcggtcgcgg gagcgctgat caagccggtg caggacggcc
     5221 tgctcggcct gttcgcaaag aaggcgaccg cggtgatgac caacgtgccg ggcccggcga
     5281 caccgctgaa gttctgcggc gcgacgctgc gccagaccat gttctgggtg ccggcatcgg
     5341 gcgacatcgg cgtgggcgtg tcgatcctgt cgtacggcgg cggcgttcag ttcgggctga
     5401 tcaccgacag gcacctgtgc ccgcacccgc agagaatcat cgaccgcttc gagcccgagt
     5461 tcgagaagct gctgctgctg gcgatgatgc tgccgtgggg gctgaggccc tgaggccggc
     5521 caagtcggtt ctcagctgcc cttgagtctt ttcacaagag ccgcgcccgt gagaagctcc
     5581 aggtgcgcct cgaggcggat cacgcgggcg gtgaggtctt cgatgcgtgc ctgttgtgcc
     5641 ttcatcgctt cggcctggcg catcaccttg tcctcgagct tgatcatcga ggtgagcgca
     5701 ccccagatct tgtcggtcac gcccatcagg cgacgtttcg ggtcggacgc ttcgtggcct
     5761 tcgcttccat cgcggcgatg cgcccgttgc tggccgcgat gaagtcgatc gcatcgtcga
     5821 tcgccgcacc ggcgcggtcg gccgcattct tcgcggcatc ggccagcgcg gccaattcgg
     5881 cgtcgctctc gctcgactca tacgagaagg cgccgcgccg catcaattcc gaaatgggga
     5941 tgtcgagctt cttcgccttg gccgcgatgg cccgtttgtc ctgcgccgtt gcctgcacga
     6001 cgattcttgc caccgctgct gccatgcgaa tctctcccgg accaacgtcg tcacgtgtcc
     6061 atggacagtg tatgtacatg gtagaagcaa tgtcaagatc aagatcgggg ccgcgccaag
     6121 accggccaga tcacgccgtt cgttttggga tgcacccgca tgcggcgccg tgattgaatc
     6181 gcgctcgcgg ctcagtcctc cagctccgcc ttcgccatct cgacgtcgag ccgctccatc
     6241 gcgtccatcg gcacacaggc cgcggcgtcc tcgtagagct gggtcgcttc cttcatcttc
     6301 ttgtcgccct cgagcatcac cagcccgttg gcgtactcga ccatcgcgat cgccgaggtc
     6361 gggttcagct tcagcgcggt cttgtacatc gtcaggccgg cgtccttgct cgcgccctgg
     6421 gtgcggccga gcagcgagcc gaccttgtcg atcacctcgg cgtggaaggc gccgagcgcg
     6481 atgtgggcgt cggcgtgctt gggcgcgagc ttgatcgcgg cctccagtgc gctcttgacc
     6541 ttgctgccca gcccctgcgc gagcgccttc gcgatgctga tgccctggct gtagcggccc
     6601 agcgcatagg ccatgaagta gtaggcgttg gcgttcttcg ggtcggtcgc cagctgcgcc
     6661 tcggcgcgct cggccacctc catgaacagg gcgagcttgg ccttctcgct cttctcgagg
     6721 tagttcgcgt agatcgcctg ggccttgttg gcggccgtga tgccggcgcc gccggccttc
     6781 atcccggcct cgacggcctc ctggaactcg ccggcgtgga acagcgccca cgcggccagc
     6841 accttcgcgt cggccggcag cggctcggcg tcgccggcgt gcaggcgcgc ccagtggcgc
     6901 tgaagcgccg cggtgtcgta gctgtagtcg gcggcgtcgt gcgggaacgc ggtccatttg
     6961 gccatgggtc agtctcctgg gttgtagttg ccgaccagcc cgtgcaggtg ctcgcgctgg
     7021 tcgggttcga gatagggcga cagcaggctg agcacgtgga acgcaccacg cagcaatgcc
     7081 gcgccggcac tttcgggctc gagcgcgttg cgcgggtcgc gcacgtactc gtagctcagc
     7141 cagtaggtca gcacgaccac catcgcggtg gcgaccggct cgatgtcggc gctgtccatg
     7201 cgcatcgcgt tgctgcgtgc caggccgtcg agcacgttct gcaccgcctt gcccttgttc
     7261 ttcagcacga actggaagtg ggtctccagg cgccggttct tcgacagcag gtcgttcagg
     7321 tcgcggtaca ggaagcggta ctgccagatc agctcgaaca gcatgtggaa gaacagccac
     7381 gcatcctcga cgttgcgcac gtcgtcggcg gcgcgcagca gctccgcgag cgcgcgctcg
     7441 tagcggtcga acaggctgtt gatcagctcg tccttggccg ggtagtggta gtacaggttg
     7501 cccgggctga tgccgagctc ggccgagatc aacgtggtcg agacgttggg ttcaccgaag
     7561 cggttgaaca gcgcgagcgt gacctccaga atgcgctcgg cggtgcggcg tggcgccttc
     7621 ttggtttgcg tggccatcgt gtcgtttgct cgtcccctgg cgggcaatct agcatcaacc
     7681 gccggcgcgc cgcggggcgc aaccctacaa tcccgcgctg catgcccgcc atctcattcc
     7741 aacacgtcgg caagacctac atcagcgcgc gcggcgcgac ccaggccctc gacgatgtca
     7801 gcttcgacat cgagccgggc gaattcttcg gccttctcgg gccgaacggt gccggcaaga
     7861 ccacgctgat cagcatcctc gccggcctcg cgcgcgccac cagcggcacg gtcaaggtgc
     7921 tcggccacga cgtcgtcgcc gactacgcgc aggcgcggcg cagcttgggg ctggtgccgc
     7981 aggagctggt gttcgacccg ttcttcagcg tgcgcgaggc gctgcgcatc cagagcggct
     8041 acttcggggt gcgcaagaac gacgcctgga tcgacgagct gctcgccaac ctcggcctgg
     8101 ccgacaaggc cgacgcgaac atgcggcaac tgtccggcgg catgaagcgc cgcgtgctgg
     8161 tcgcgcaggc gctggtgcac cgcccgccgg tgatcgtgct cgacgagccg accgccggcg
     8221 tcgacgtgga gttgcgccag acgctgtggc acttcgtcgc gcgcctcaac aaggaaggcc
     8281 acaccgtgct gctgaccacg cactacctcg aagaggccga ggcattgtgc gggcgcctgg
     8341 cgatgctcaa gcagggccgc gtggtcgcgc tggaccgcac ctcggcgctg ctggccggca
     8401 ccgccagcac gatgttgcgc ttcaagatgg acgcgccgct gccggcgtcg ctggcggcgc
     8461 agtcgcgcgt gaccgggcgc atcgtgcaga tcaccgcaca cgacgcgcgc gaggtcgaga
     8521 ccatcctggg cgtgttgcgc aacgccggct gccagcccga ggacctggag atcggtcgcg
     8581 ccgatctcga ggacgtgttc ctcgagatca tgagcggaac gccgcgcgcg gcgccgccgc
     8641 tgggggtcgc cgcgtgacgc cggttcatgt gctgtcgggc gcggacacgc tgctctacaa
     8701 ggaggtgctg cgcttctgga aggttgcgtt ccagaccgtc gccgcgccgg tgctgaccgc
     8761 gatcctgtac ctgctgatct tcggccatgt gctcgaggac cgcgtcaagg tgttcggctc
     8821 ggtcggctac accagcttcc tgatcccggg gctggtgatg atgagcgtgc tgcagaacgc
     8881 gttcgcgaac agcagctcgt cgctgatcca gagcaagatc accggcaacc tggtgttcct
     8941 gctggtcgcg ccgctgtcgc actgggcctg gttcgtggcc tacgtgggcg cgtcggtggt
     9001 gcgcggcgcg gtggtcggtg ccggcgtgat gatcgccacc gtctggttcg cgccgctgca
     9061 cctggcgcag ccggggtgga tcgtcgtgtt cggtgtggcc ggcgcggcgc tgatgggcgc
     9121 gctcgggctg atggccgggc tgtgggccga gaagttcgac cagatcgcgg cgttccagaa
     9181 cttcatcatc atgccgatga cgtttctgtc gggcgtgttc tattcggtcg gctcgatgcc
     9241 gccgttctgg agtggcgtga gccacctgaa cccgttcttc tacatgatcg acggctttcg
     9301 ccacggcttc ttcggcgtca gcgacgtgtc gccgtggacc gggctg
//
LOCUS       DATMJB010000509         2998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_598101, whole genome shotgun sequence.
ACCESSION   DATMJB010000509 DATMJB010000000
VERSION     DATMJB010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598101"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VNU48_10945"
     CDS             <1..1572
                     /locus_tag="VNU48_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010090523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HWI11838.1"
                     /translation="PQVVKSARVMKSAVAHLLPYIEEEKRLLIEGGGDAKPKGKIVIA
                     TVKGDVHDIGKNIVTVVLQCNNFEVVNMGVMVACQDILARAKVEGADLIGLSGLITPS
                     LEEMQHVAAEMQRDEFFRVKKIPLLIGGATTSRVHTAVKISPHYEGPVVYVPDASRSV
                     SVCSDLLSDDRAAAYINELNADYIRVREQHANKKATPLVTLAAARANKTPVDWSSFTP
                     AKPKFIGRRVFRNQDLAEIAACIDWGPFFQTWDLAGAYPAILNDEIVGESARRVLSDG
                     QRLLRRAIEGRWLTANGVIGLYPANSVNDDDIEIYTDASRERVLMTWRGLRMQSVRPV
                     VDGVMRPNRCLADFIAPKDTGIADHIGLFAVTAGLGAEKKDKQFLADLDDYSSIMFKA
                     LADRLAEAFAERLHQRVRTEFWGYAADEQLSNAELIAEKYSGIRPAPGYPACPDHTVK
                     AEMFRVLDCADIGMGLTESLAMTPAASVSGFYLAHPQATYFNVGKIGEDQLADWARRS
                     ALATDLVRRSLSSVL"
     gene            complement(1603..2196)
                     /locus_tag="VNU48_10950"
                     /pseudo
     CDS             complement(1603..2196)
                     /locus_tag="VNU48_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2379..2633)
                     /locus_tag="VNU48_10955"
     CDS             complement(2379..2633)
                     /locus_tag="VNU48_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI11839.1"
                     /translation="MKKRKRAQNAREVLAENLKRLRAEHRLSQEGLAEKAGFHRTYVS
                     QVERCKTNISLDNLEKLATYLAVPTYSLLVELDASPSGPP"
BASE COUNT          520 a          973 c         1010 g          495 t
ORIGIN      
        1 ccccaggtgg tgaagagcgc ccgcgtcatg aagagtgccg tggcccacct gctgccctac
       61 atcgaggaag aaaagcgcct gctcatcgaa ggcggcggcg atgccaagcc caagggcaag
      121 atcgtcatcg cgacggtcaa gggcgacgtg cacgacatcg gcaagaacat cgtcacggtg
      181 gtcctgcagt gcaacaactt cgaggtcgtc aacatgggcg tcatggtggc gtgccaggac
      241 atcctggccc gcgccaaggt cgagggggcc gacctgatcg gcctgtcggg cctgatcacg
      301 ccgagcctcg aggagatgca gcacgtcgct gccgagatgc agcgcgacga gttcttccgc
      361 gtgaagaaga tcccgctgct gatcggcggc gcgacgacga gccgcgtgca cactgcggtg
      421 aagatttcgc cgcactacga agggccggtc gtctacgtgc cggacgcgag ccgttcggtc
      481 agcgtctgca gcgacctgct cagcgacgat cgcgcggcgg cctacatcaa cgagctgaac
      541 gccgactaca tccgcgtgcg cgagcagcat gcgaacaaga aggcgacgcc gctggtgacg
      601 ctggccgctg cgcgcgcgaa caagacgcct gtcgactggt cgtcgttcac gccggcgaag
      661 ccgaagttca tcggtcgccg cgtgtttcgc aaccaggacc tggccgagat cgccgcgtgc
      721 atcgactggg gcccattctt ccagacctgg gacctcgcgg gcgcgtaccc ggcgatcctc
      781 aacgacgaga tcgtcggcga gtcggcgcgg cgcgtgctga gtgacggtca gcgcctgctg
      841 cgccgcgcga tcgaggggcg ctggctgacc gccaacggcg tgatcggcct gtacccggcc
      901 aacagcgtga acgacgacga catcgagatc tacaccgacg cgtcgcgaga gcgagtgctg
      961 atgacctggc gcggcctgcg catgcagagc gtgcggccgg tcgtcgatgg cgtgatgcga
     1021 ccgaaccgct gtctggccga cttcatcgcg cccaaggaca ccggcatcgc cgaccacatc
     1081 ggcctgttcg cggtgaccgc tgggctgggc gccgagaaga aggacaagca gttcctcgcg
     1141 gacctcgacg actacagctc gatcatgttc aaggcgctgg ccgatcggct cgccgaggcc
     1201 ttcgccgaac gcctgcacca gcgcgtgcgc accgagttct ggggctacgc ggccgacgaa
     1261 cagctgagca acgccgagct gatcgccgag aagtattcgg gcatccgccc ggcgcccggc
     1321 tacccggcct gcccggacca cacggtgaag gccgagatgt tccgcgtgct ggactgcgcc
     1381 gacatcggca tgggcctgac cgagagcctg gcgatgacgc cggccgccag cgtcagcggc
     1441 ttctacctgg cgcacccgca ggcgacctat ttcaacgtcg gcaagatcgg cgaggaccag
     1501 ctcgccgact gggcccggcg cagcgcgctg gccaccgatc tggtgcggcg ctcgctttcg
     1561 tcggtgctct gacccgacgg gcgcgccgac cggccgcggc gctcacgacc ggatcagcac
     1621 gcggcggtac tggatcgcct cggcgaggtg gctgatcgcg atcgcgtcgc tgcccgccag
     1681 gtcggcgatg ctgcgcgcga cgcgcagcac gcggtggaag ccgcgcgccg accagcccag
     1741 gcgcgccgcc gcggtgcgca agaactgcgt accggccgcg tcgagcgcgc cgtgccgatc
     1801 gagcgcctca ccggtcagcg ccgcgttgag gctgtcctgc cgcgccagcg cacgctcgcg
     1861 cgcagcggcc acccgctgcg cgatcacgtg gctggcctcg ccgtcgggcg ccgacgccaa
     1921 ggcttcgggc gcgaccgccg gcacctcgac ctggacgtcg atgcggtcca gcagcggccc
     1981 gctgatgcgg ccctggtagc gcgccaccac atcgggcgtg cagcggcagg cgtgcaacgg
     2041 gctgccgagg tggccgcagg ggcaggggtt catcgccgca atcaactgga agcgcgccgg
     2101 gaactcggcc tggcgcgccg cgcgcgagat cacgatgcgg ccggtctcga gcggctcgcg
     2161 cagcgcctcg agcgcggcgc gcgcgaactc ggggagttag cttttcgacc caattcaatg
     2221 tcaattatct agtagggcaa gcccgcggga gcgggcgcgt caggtgtttc gtgttgggga
     2281 gggccgggag gggcggaggg ttgtggataa agctgtgcat aacacttgaa taagtcaccg
     2341 cggagctcag tctccctgtc cgcttttctg agcgacgact acggcgggcc ggaagggctc
     2401 gcgtcgagtt cgaccaacag ggagtacgtc ggaacagcga ggtaagtcgc gagcttctcg
     2461 aggttgtcca agctgatatt ggttttgcag cgttcgactt gactgacgta ggtgcggtgg
     2521 aagcccgctt tttcagcgag tccctcttgc gaaagtctgt gctctgctct caaccgtttc
     2581 aggttttcag cgaggacctc tcgtgcgttc tgcgcgcgct tccgcttttt catggaagcg
     2641 agggtcgcta aagaccgtct aaaaatctac agatgatgca tctactgctt ttacatctac
     2701 ttgttttgga tatactttgc gcatgacaat gcccgacgaa agaacgcgtg cagtcgtgca
     2761 aacgcgcgag ttcttgtgtg ccgttgtcga gtcgccgttg gaggttccga cggaactacg
     2821 ccggcatgcg ctgcaactcc tcaggcatta cccgatgcca ggtgtcatgg acctcgtgca
     2881 catcgctctt ccagcctggt tcgggcccga gagtgcccag taggttcagt gaacctctgg
     2941 gtcctcactg aaagcaagca tgttcgtcac ttcaccaaca ttcgaagagc acgtacca
//
LOCUS       DATMJB010000510         6283 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_731208, whole genome shotgun sequence.
ACCESSION   DATMJB010000510 DATMJB010000000
VERSION     DATMJB010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6283
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731208"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VNU48_10960"
     CDS             <1..262
                     /locus_tag="VNU48_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HWI11840.1"
                     /translation="LVADLVGWARDLMLAGAPLVHSIPGRAGWELRLVVQGGLRILEK
                     IADSGFATLYRRPALRWYDAPLLAWRALWMRPSQPLVASESA"
     gene            339..1175
                     /gene="hpnD"
                     /locus_tag="VNU48_10965"
     CDS             339..1175
                     /gene="hpnD"
                     /locus_tag="VNU48_10965"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HWI11841.1"
                     /translation="MTPEQYVQDKAAKSGSSFYYAFLFLPPPRRAAITAFYAFCREVD
                     DVVDEVSDPGVAATKLAWWRTEVAAAFAGRPSHPATKALMPHTAAFDIRAEHLQAVID
                     GCQMDLEQSRYLDFAGLARYCHLVAGVVGEVAAGIFGQTQAATTAYAHRLGLAMQLTN
                     IIRDVGDDARRGRIYLPVSELKQFDVKAQEILNRGYSERFTALMKFQAERAHRCYDEA
                     FALLPDADRPAQKPGVMMANIYRTLLREIEADDFQVLHQRTSLTPLRKLWIAMRTNWR
                     GR"
     gene            1177..2448
                     /gene="hpnE"
                     /locus_tag="VNU48_10970"
     CDS             1177..2448
                     /gene="hpnE"
                     /locus_tag="VNU48_10970"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HWI11842.1"
                     /translation="MASGRLAVVGGGWAGLAAAIEGVRRGHAVTLFEMAPQLGGRARG
                     VDVDGLTLDNGQHILIGAYVETLALLQTVGVAASEAFLRTPLRLRYPDGNGLHLRPGP
                     PALAFAGAALRQHGWRLHARLALLVAAGGWAARGFRCDPALTVATLTRALPASVRDEL
                     IDPLCVAALNTPAAQASASVFLRVLKDALFSGPGSADLLLPRVGLSALLPAPAQRWLG
                     EAGADLRLQCRVETLTRRDDAWLLGDEPFDHVVLATPPGEAARLTRAIAPSWSSTAAA
                     LRYEPIVTVYLSGDGVRLPEPMLALRSDARAPAQFVFDRGQLGGPAGLLAFVISGAQP
                     WVDRGMEASTQATLAQAHAVLGVHLRAPLVALRTLTEKRATFRCTPDLARPGMQIAPG
                     LHAAGDYVAGPYPATLEGAVRSASQAVRALR"
     gene            complement(2470..3579)
                     /locus_tag="VNU48_10975"
     CDS             complement(2470..3579)
                     /locus_tag="VNU48_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HWI11843.1"
                     /translation="MLVALAPLAVLPARAQNGDDYLRRPYDFSAAPAATAPSRAPQWL
                     QIPRADGRLTSRQLGLMINTADPYSVEVGEFYIKARGLTDAQVLRVQLPLRAALTPEE
                     FKAFSAAVDAFFGASVQALALAWTQPYAVGCNSITGALALGFDAALCEHSCAASPRSP
                     YFDNASTHPYTDLKMRPAMLLAARDVPTAEAMIRRGVAADGSLGLRGAPPVHAYFVTT
                     SDRARSVRSGLFPPAGPLRRRGVEVHVETTQAIENAQRVLIYQTGLARVPKLDTLKWV
                     PGALADHLTSFGGVLGSANGQMSILDWIGSGATASYGTVSEPCAHPQKFPHPQVLLLN
                     YLQGATAIEAYWRSVAWPQQGVFIGEPLAAPFARR"
     gene            3680..4447
                     /locus_tag="VNU48_10980"
     CDS             3680..4447
                     /locus_tag="VNU48_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWI11844.1"
                     /translation="MAKGDIKTPTIQVLARAFGLLDVLASYPDPVSLKQISEQTGLHP
                     STAHRILNDLTIGRYVDRPEAGSYRLGMRLLELGNLVKARLDVRDAALGPMRELHKLT
                     HQPVNLSMRQGDEIVYIERTYSERSGMQVVRAIGGRAPLHLTSVGKLFLAHDDAQRVR
                     AYATRTGLAGHTRNSITELAQLERELARVNQYGTARDDEELELGVRCMAASILDDQGK
                     LVAGLSISAPADRLEDSWLERLKETAAQISAALGHRR"
     gene            complement(4456..5523)
                     /gene="pbpG"
                     /locus_tag="VNU48_10985"
     CDS             complement(4456..5523)
                     /gene="pbpG"
                     /locus_tag="VNU48_10985"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HWI11845.1"
                     /translation="MTTGVLVTLAALSLTLTAHADAAERVAAKKKSHAVAPLKNKAVR
                     SDKPARPVAVLRNGRSGSLKRVSSVMRVEPASPSFGQLYGLHNTADALDLKSSVALVL
                     DSDTDEVLFSKNPSAVLPIASLTKLMTAVVVTEAKLPLNETLTITDDDVDTEKNSRSR
                     LRVGTTLSREEMLHLALMSSENRAAHALGRAYPGGLEAFVGAMNAKARELGMNDTHYV
                     EPTGLSSRNQSSARDLATLVKAAQEHALIREFSTSPEYEVEVGKRTTQFRTTDALVRS
                     PSWDILLQKTGYITEAGRCLVMQARMAGRKLIMVFLDSAGKHSHIGDAERVRRWVAEL
                     PARAPAVVAPAADALLAKPAT"
     gene            complement(5666..>6283)
                     /locus_tag="VNU48_10990"
     CDS             complement(5666..>6283)
                     /locus_tag="VNU48_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HWI11846.1"
                     /translation="RDTYEIMRAEDVGWAANKIVLGKLSGRNAFKQRLKELGIEMASE
                     AEINAGFVRFKELADRKSDIFDEDIIALVMDESVTAEHEHYRLLALSQRSEMGERPHA
                     SVAFAAGEVEHHVEADGNGPVDASLKAIESKLQSGAEMLLYSVNAITSGSTESQGEVT
                     VRLQLGGRVVNGVGADPDIVVASAKAYLSALNKLHSKIERVAAQG"
BASE COUNT          942 a         2174 c         2177 g          990 t
ORIGIN      
        1 gctggtcgcc gacctcgtcg gctgggcacg cgacctgatg ctcgccggtg cgccgctggt
       61 tcacagcatt cccggccgtg ccggctggga gctgcggctg gtcgtgcagg gcggcctgcg
      121 catcctcgag aagattgcgg acagcggctt cgcgaccctg taccgtcgcc cggcactgcg
      181 ctggtacgac gcgccgctgc tggcctggcg cgcactttgg atgcgcccat cgcagccgct
      241 cgtcgcgagc gagtccgcat gacgcccgag cagtacgtgc aggacaaggc cgcgggggcc
      301 accagttgtc ggccttgccc tgcccgcgag gatggcgcat gacgcccgag cagtacgtgc
      361 aggacaaggc cgccaagagc ggctcgagct tctactacgc gttcctgttc ctgccgccgc
      421 cacggcgcgc cgcgatcacc gcgttctacg cgttctgccg cgaggtcgac gacgtcgtcg
      481 acgaggtcag cgacccgggc gtggcggcaa ccaagctggc gtggtggcgc accgaagtcg
      541 cagccgcgtt tgcggggcgg cccagccacc cggcgacgaa ggcgctgatg ccgcacaccg
      601 ccgccttcga catccgtgcc gagcatctgc aggcggtgat cgacggctgc cagatggatc
      661 tggagcagtc gcgctacctc gatttcgccg gcctcgcacg ctactgccat ctggtcgccg
      721 gcgtcgtcgg cgaagtggcg gctggcatct tcgggcagac gcaagccgcg acgacggcct
      781 acgcgcaccg gctcggcctg gcgatgcagc tcaccaacat catccgcgac gtcggcgacg
      841 acgcgcggcg cgggcgcatc tacctgccgg tgtcggagct gaagcagttc gacgtgaagg
      901 cgcaggagat cctgaaccgc ggttacagcg agcgtttcac ggcgctgatg aagttccagg
      961 ccgagcgcgc gcaccgctgc tacgacgagg ccttcgcgct gctgcccgac gccgatcgac
     1021 cggcgcaaaa gccgggcgtg atgatggcca acatctaccg cacgctgctg cgcgaaatcg
     1081 aggccgacga cttccaggtg ctgcaccagc gcacctcgct gacgccgctg cgcaagctgt
     1141 ggatcgcgat gcgcacgaac tggcgcggcc gctagcgtgg cgagcggcag gctcgcggtc
     1201 gtcggcggcg gttgggccgg gctggccgcg gccatcgagg gtgtgcgccg cggccacgcg
     1261 gtcacgctgt tcgagatggc gccgcagctc ggcgggcgcg cccgcggcgt cgacgtggac
     1321 ggcctgacgc tcgacaacgg ccagcacatt ttgatcggcg cctacgtcga gacgctcgcg
     1381 ctgctgcaga cggtgggcgt ggcggcgagc gaggcattcc tgcgcacgcc gctgcgcctg
     1441 cgctaccccg acggcaacgg cctgcatctc aggcccggcc cgccggcact cgcgttcgcc
     1501 ggcgcggcgc tgcgccagca cggctggcgc ctgcacgcgc gtctggcgct gctggtcgcg
     1561 gccggcggct gggccgcgcg cggttttcgc tgcgacccag cgctgacggt ggcaacgttg
     1621 acgcgcgcct tgcccgcgtc cgtccgcgac gaactgatcg atccgctgtg cgtcgcggcg
     1681 ttgaacacgc cggcggcgca ggccagtgcg agtgtgtttc tgcgcgtgtt gaaggatgcc
     1741 ctgttctccg gccccggctc ggccgacctg ctgctgccgc gtgtcggcct gagcgcgttg
     1801 ctgcccgcgc cggcgcaacg ctggctgggc gaggcgggcg ccgaccttcg gctgcagtgc
     1861 cgcgtcgaga cgctgacgcg tcgtgacgac gcctggctcc tcggcgacga accgttcgac
     1921 cacgtcgtgc tcgccacgcc gcccggtgaa gccgcacgcc tgacgcgcgc gatcgcgccg
     1981 tcgtggtcgt caaccgccgc ggcactgcgc tacgagccga tcgtcacggt ctacctgagc
     2041 ggcgacggcg tgcgcctgcc cgagccgatg ctggcgctgc gctcggacgc gcgcgcgccg
     2101 gcgcagttcg tgttcgaccg tggtcagctc ggcggcccgg cgggcctgct ggcgttcgtg
     2161 atcagcggcg cccagccttg ggtcgatcgg ggcatggagg ccagcacaca ggcgacgctg
     2221 gcgcaggcgc acgcggtgct gggtgtgcac ttgcgcgcgc cgctcgtggc gctgcgcacg
     2281 ctgaccgaga agcgggcgac gtttcgctgc acgcccgatc tcgcgcgacc cgggatgcag
     2341 attgcgccgg gcctgcacgc ggcgggcgac tacgttgcgg ggccgtaccc ggcgacgctc
     2401 gaaggtgcag tgcgcagcgc gtcgcaggcc gtgagggcac tgcgctgacg cacgcgcgga
     2461 cgcgctgctt cagcgccgcg cgaacggcgc cgccagcggc tcgccgatga acacgccctg
     2521 ctgcggccag gccacgctgc gccagtaggc ctcgatcgcg gtcgcgcctt gcagatagtt
     2581 cagcagcagc acctgcggat gcggaaactt ctgcgggtgc gcgcagggct cggacacggt
     2641 gccgtagctc gcggtcgcgc ccgagccgat ccagtcgagg atcgacatct ggccgtttgc
     2701 gctgccgagc acgccgccga acgaggtcag gtgatcggcg agcgcgcccg gcacccactt
     2761 cagcgtgtcg agcttgggca ccctggccag cccggtctgg tagatcagca cgcgctgcgc
     2821 gttctcgatc gcctgcgtcg tctcgacatg cacctccacg ccccggcggc gcagtggccc
     2881 ggccggcggg aacagcccgg agcgcacact gcgcgcgcgg tcgctggtcg tcacgaagta
     2941 ggcatggacc ggcggtgcgc cgcgcagacc gaggctgccg tcggcggcca cgccgcgccg
     3001 gatcatcgcc tcggccgtcg gcacgtcgcg cgccgcgagc agcatggccg gtcgcatctt
     3061 cagatccgtg tacggatgcg tcgaggcgtt gtcgaagtac ggcgagcgcg gtgacgccgc
     3121 gcagctgtgt tcgcacaacg ccgcatcgaa gccgagcgcg agcgccccgg tgatcgagtt
     3181 gcatccgacc gcgtagggct gggtccatgc cagcgccaag gcctgcacgg acgcgccgaa
     3241 gaacgcatcg acggccgcgc tgaacgcctt gaactcctcc ggggtcagcg ccgcgcgcag
     3301 cggcaactgc acgcgcagca cctgtgcgtc cgtcagaccg cgagccttga tgtagaactc
     3361 accgacctcc accgagtagg ggtcggcggt gttgatcatc agaccgagct gccggctggt
     3421 cagacgcccg tcggcacgcg gaatctgcag ccactgcggc gcgcgcgacg gcgcggtggc
     3481 ggccggcgcg gcgctgaagt cgtacgggcg ccgcagataa tcgtcgccgt tctgggcccg
     3541 cgcaggcagc actgccagcg gcgccagcgc caccagcacg agggttccga tgaaggtacg
     3601 aagcctgctt cgcgctctcg gatcgcagcc gagtgtttcg ccatacaaaa atgcaacgac
     3661 ttgagcgaga atgacattca tggcgaaagg cgacatcaag actccgacga tacaagtgct
     3721 ggcgcgtgcc ttcggactgc tcgacgtgct ggcgagctac cccgatccgg tctcgctgaa
     3781 gcagatcagc gagcagaccg ggctgcaccc ttcgaccgcg caccgcatcc tcaacgacct
     3841 gacgatcggc cgttatgtcg atcggccgga agccggcagc tatcggctcg gcatgcgttt
     3901 gctggaactc ggcaacctgg tcaaggcgcg cctggacgtg cgcgacgccg cgctcggccc
     3961 gatgcgcgag ttgcacaagc tgacgcacca gccggtgaac ctgtcgatgc gccagggcga
     4021 cgagatcgtc tacatcgaac gcacctacag cgaacgctcg ggcatgcagg tggtgcgcgc
     4081 catcggcggc cgtgcgccgc tgcacctgac ctcggtcggc aagctgttcc tggcccatga
     4141 cgatgctcag cgcgtacggg cctatgcgac gcgcaccggc ctggccggcc acacgcgcaa
     4201 cagcatcacc gaactcgcac agctcgagcg cgaactggcg cgcgtgaacc agtacggcac
     4261 ggcgcgcgac gacgaggaac tggaactggg cgtgcgctgc atggccgcga gcatcctcga
     4321 cgaccagggc aagctggtcg caggcctgtc gatctcggcg ccggcggacc ggctcgagga
     4381 cagctggctg gagcgtctga aggaaacggc tgcgcagatc tcagcggcgc tcgggcatcg
     4441 gcgctgagcg ctcgctcagg tcgccggctt ggccagcaac gcgtccgcgg ccggcgccac
     4501 cacggccggc gcgcgggccg gcagttccgc gacccagcgg cgcacacgct cggcatcgcc
     4561 gatgtgcgaa tgcttgcccg ccgaatccag gaacaccatg atcagcttgc gcccggccat
     4621 gcgcgcctgc atcaccaggc agcgaccggc ctcggtgata tagccggtct tttgcagcag
     4681 gatgtcccac gacgggctgc gcaccagcgc gtcggtggtg cggaactggg tcgtgcgctt
     4741 gcccacttcg acctcgtatt ccggtgaggt cgagaattcg cggatcaggg cgtgttcctg
     4801 cgccgccttc acgagcgtcg cgaggtcgcg cgcgctggac tggttgcggc tcgacagacc
     4861 cgtcggctcg acgtagtgcg tgtcgttcat gccgagctcg cgcgccttgg cgttcatcgc
     4921 gccgacaaag gcctcgagcc cgcccgggta ggcgcggcct agtgcatgcg cggcacggtt
     4981 ctcggacgac atcagcgcca gatgcagcat ctcctcgcgg ctcagcgtgg tgcccacgcg
     5041 cagccgcgac cggctgttct tctcggtgtc gacgtcgtcg tcggtgatcg tcagggtctc
     5101 gttcagcggc agcttcgctt cggtgacgac gaccgccgtc atcagcttgg tcagcgacgc
     5161 gatcggcaag accgcgctcg ggttcttgct gaacaggact tcgtcggtgt cggagtcgag
     5221 caccagcgcg acgctggact tcaggtcgag cgcgtccgcc gtgttgtgca ggccatagag
     5281 ctggccgaac gacgggctgg ccggctcgac gcgcatcacc gacgagacgc gcttgaggct
     5341 gccggaacgg ccattgcgca gcactgcgac cggacgcgcc ggcttgtcgg agcgcaccgc
     5401 cttgttcttc agcggggcaa ccgcatgcga cttcttcttg gccgcaacgc gttcggcggc
     5461 atctgcatgc gccgtcaagg tgagcgacag ggcggccagt gtgaccagta cgccggttgt
     5521 cacaaacttc ttgatcgatg ccacagcagt cccttcacgc ccagtgcggc gagtctatgt
     5581 gaaacaaaaa aaacgcgcaa gatcaaaaac ttacgcgctt ttcctcaagt cgggacttgg
     5641 gagtggtcag accgtgaaaa aggtctcaac cctgggcggc aacgcgttcg atcttgctgt
     5701 gcagcttgtt caacgccgag aggtaggctt tcgccgacgc cacgacgatg tccgggtctg
     5761 cacccacgcc gttgacgacc cggccgccga gttgcagccg caccgtcact tcgccctggg
     5821 attctgtgct gcccgaggtg atggcattga ccgaatagag cagcatttcg gcgccagatt
     5881 gcagcttcga ctcgatcgcc ttcaggctcg cgtcgaccgg accgttgccg tccgcctcga
     5941 cgtggtgctc gacctcgccg gccgcgaacg cgacgctcgc gtgcggtcgc tcgcccatct
     6001 cggaacgttg cgagagcgcc agcagccggt agtgctcgtg ctcggcggtg accgattcgt
     6061 ccatcaccag cgcgatgatg tcttcgtcga aaatgtcgct tttgcggtcg gccagttcct
     6121 tgaagcgcac gaagccggcg ttgatctcgg cctcactggc catctcgatg ccgagctctt
     6181 tcaggcgctg cttgaacgca ttgcggcccg agagcttgcc gagcacgatc ttgttcgcgg
     6241 cccagccgac gtcctcggcg cgcatgatct cgtaggtgtc gcg
//
LOCUS       DATMJB010000511         7485 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_799023, whole genome shotgun sequence.
ACCESSION   DATMJB010000511 DATMJB010000000
VERSION     DATMJB010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_799023"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VNU48_10995"
     CDS             <1..479
                     /locus_tag="VNU48_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11847.1"
                     /translation="LALADWRHCGAVAIPRLGCFVLDARRAEVLAYLQRHAKAGDLLY
                     VGTGRHDKLFVNNVELYFLSGLAAATKWHDLHPGVQTTRALQSAMIAEMNLHPPAFVV
                     LNAEWDDRAEPNLSRVSSGVTLLDDYLRERFVVELRAGSFTVSVPRGPAPRAGPAP"
     gene            476..937
                     /locus_tag="VNU48_11000"
     CDS             476..937
                     /locus_tag="VNU48_11000"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWI11848.1"
                     /translation="MSRPSSSPPGAAEAIRSAWHTRQRFLRFLLAAGASVPVNIAARI
                     ALSNVVRYEIAVLLSHLVGMLTAYTLTRLFVFEKSGRPVRSELTRFAVVNIYSAAQTW
                     VVSVGLVYWVFPWIGFETWPELVAHVIGLALASVTSFVAHSRYSFRRADLS"
     gene            complement(934..2163)
                     /locus_tag="VNU48_11005"
     CDS             complement(934..2163)
                     /locus_tag="VNU48_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11849.1"
                     /translation="MKPPTLRDPRTRPALLLALMLVVASMRWIASAGGRSELFTTDSY
                     LLYQAATLGFNYFELGAVRRGLGGSIVHLLNPDMLIATLLFHVLSAAAVAVAAGLLFR
                     RIAGPPWIQAVFAFTAVTIMLRWAEDAVGRTDMSIAALLAFATLAMTAGRLMLACALV
                     CIGLFIHETSFIFGLPLLAALLLRRGGWSAWRATDKAWAGLAFAVALALYAGMAWLPH
                     VDTQTAIDTVRAKFAAHRFVDWGIYFAASGSRGVRTSICQNMIDPNFWLHVGGGLVVL
                     LVIGVALGSRIRAEWPAMLLASLPPFLFLCVVANDNARWITLACFNVWLVAAATPDRN
                     GRNDVPARLAVAAAFALLLLTHPRLGRSQTPMFEASPLLERLSRQLGGPRTPTFAQAL
                     QRCDPGWRDALGPASPH"
     gene            2255..3994
                     /locus_tag="VNU48_11010"
     CDS             2255..3994
                     /locus_tag="VNU48_11010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWI11850.1"
                     /translation="MKRSAVSAAGLVAAGLLLALLVVNPLRELLTQDDGWAYARMVER
                     LLKTGEYRLDAWSAANMPVQIYLAAGLAEVFGYSLSLLRCTTLLLLAVGLASLYALLR
                     ELGTDRGHAALLTLGLLASPLVLMLAFTFMSDAQFLGWMLLALWLYVRGLRVESDALV
                     FCGSLAAACAIGTRQFGIAIMAGLALTWLVSNRQRRPPMHRLLIALAVPMLVGVWQLQ
                     AGLREPNFTQAYRVADQRLFLAHSVATLAREGLWRSVTLVHYLGLSLLPLAPLLAGLA
                     LRRGAPRAAGARFQRWPVVLATLLVAAGLVWASVAGSPLTARPADPGETDPPPLPLYW
                     MLPTAFAARHRRMTLLLDACGGVLALLFAWVVLQAWWARRPDFRALRHGPPGVSLLLG
                     TGLSLLVLQLSYVQFNDTYLAGLLPFALLLLVPALRATPPRGWAAATGACAYAAILVT
                     SLWMRGDYNRQQAYWDGAERLLRSGHAPRDIGGELHWAEYHGAFDEWIAAGAPGLERA
                     GEGSSKAVYGPLHDPFYRWVEFRSWRAGYRLQEESQGPPPAGWQLVDTVPYRDGYFAA
                     RSIWILARRAARD"
     gene            4007..5437
                     /locus_tag="VNU48_11015"
     CDS             4007..5437
                     /locus_tag="VNU48_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI11851.1"
                     /translation="MPSWGFGDARKPVDSARVAPGRGAEGPAPVTNAKNRAQGLPSSP
                     RIAVIGGGPMGLAAALELAKKGARVEVLERDDRLGGMSAHFDFSGTRIERYYHFICAP
                     DQTTFQYLRELDLEDKLRWTDTRMGFYLNGQLHDWGNPQAMLRFPGLSLLQKLRYGLH
                     VMRAKSITDWRPYDTLTSTEWVQKWIGKPAYEVMWRSLFHYKFYEFQDSLSAAWLGTR
                     IKRVALSRKSIFQERLGYLDGGSEVLLEAIAARLTQCGGSLSLASPVEAVLTEGAGST
                     RRVRGILAGGVELAFDAVVSTAPLPHVPALVPDLPGDERAKIDAIRNVGVVCVILKLK
                     RSFTPNFWLNINDPRVQIPGLIEYTNLNPLDGSSIVYAPFYMPHSHPKYQRTNQEFID
                     ETLACLSVIRPDFDRDDVLAATASRYRFAQTVCSPGFLEALPPMRSGLKGFFMADTSY
                     YYPEDRSISESLRTGARLAHTVWEAL"
     gene            5455..6906
                     /locus_tag="VNU48_11020"
     CDS             5455..6906
                     /locus_tag="VNU48_11020"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HWI11852.1"
                     /translation="MPAAYAAEVPAPTLVVDLDGTLIRSDLLHDAVVQLVLQKPSTLW
                     SMPGWLLRGKAHFKAQVASRTRLEVDGLPYEPALVEWIRSERASGRSVILCTASDVRQ
                     AHLVARATDLFDDVMASDGTTNLSAQRKADALVQRFGWQGFDYAGNSRADVPVWKASR
                     RAIVVGATASTRAAAAEVAVVEREFAPRTGRLRAWFSALRVQQWVKNLLVFVPLLGAH
                     EFQNLPALLSVALAFVAFSLCASSVYLINDIVDLNSDRGHPRKRGRPFASGALPIPHG
                     IVVSLLLMATAFALALASVNLEFVAWLAAYLVTTACYSLWLKRKILVDSLVLAALYTL
                     RLLAGGAAAGTATGFWLLAFSLFLFLSLALVKRYSELRETFRRGRARTPGRDYIIEDL
                     PLVQMLGIASGFSSVMVLALYINGDTVARLYPHEQMIWFTVPVLLYWVSRLWLKAHRG
                     ELDDDPLVFALSDNLSRVTILMFFGSLLLASRH"
     gene            7011..>7485
                     /locus_tag="VNU48_11025"
     CDS             7011..>7485
                     /locus_tag="VNU48_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWI11853.1"
                     /translation="MPAIAHGNGRSYGDEALNGGGTVWLARGLDKFIGFDADRGVLRC
                     EAGVSLREIIDVVLPRGWFLPVTPGTQFVTLGGAVANDVHGKNHHRCGTLGEHVTALT
                     LLRSDGQVIDCGPAARSDWFCATVGGMGLTGVILDVTLQLRRVPGPWIETRTHAFQ"
BASE COUNT         1047 a         2551 c         2617 g         1270 t
ORIGIN      
        1 cgctggcgct ggcggactgg cggcactgcg gggccgtggc gataccgcgg ctcgggtgct
       61 tcgtgctgga tgcgcgccgc gcggaggtcc tcgcgtacct gcagcgccac gcgaaggcgg
      121 gcgaccttct gtacgtgggc acgggccgcc acgacaagct gttcgtcaac aacgtcgagc
      181 tctatttctt gagcggcctg gcggcggcga cgaagtggca cgacctgcat cccggcgtgc
      241 agaccacgcg cgcgctgcag tcggcgatga ttgccgagat gaacctgcac ccgccggcct
      301 tcgtcgttct caacgccgaa tgggacgatc gcgccgagcc caacctgagc cgcgtgagca
      361 gcggcgtgac gctgctggac gactacctgc gcgagcgctt cgtcgtcgaa ctgcgcgccg
      421 gcagcttcac ggtgtcggtg ccgcgtgggc cggcgccgcg ggccggccct gcgccatgag
      481 ccgaccgtca tcgtccccgc caggcgctgc cgaagcgatc cgctcggcat ggcatacgcg
      541 ccaacgcttc ctgcgcttcc tgctcgccgc gggcgcttcg gtgccggtga acatcgcggc
      601 gcgcatcgcc ctgtcgaacg tggttcgcta cgagatcgcg gtgctgctgt cgcacctggt
      661 gggcatgctg accgcgtaca cactgacccg gctgttcgtg ttcgagaagt cgggccgccc
      721 ggtgcgcagc gaactcacgc gttttgcggt cgtcaacatc tactcggcgg cgcagacctg
      781 ggtggtcagc gtcggcctgg tctactgggt gtttccgtgg atcggtttcg agacctggcc
      841 cgaactggtc gcccatgtca tcgggctggc gctggcgtcg gtgacttcgt ttgtcgccca
      901 cagccgctat tcgtttcgac gtgccgacct atcctaatgc ggcgacgccg ggccgagcgc
      961 gtcgcgccag cccggatcgc agcgctgcag cgcctgggcg aaggtgggcg tgcgcggccc
     1021 gccgagctgg cgcgagaggc gctcgagcag cggactggcc tcgaacatcg gggtctggct
     1081 cctgccgagc ctgggatggg tcagcagcag caacgcgaac gccgcggcga ccgccagccg
     1141 cgccgggacg tcgttgcgcc cgttgcgatc gggcgtcgcc gccgcgacca accagacgtt
     1201 gaagcaggcc agggtgatcc agcgtgcgtt gtcgttggcc accacgcaca gaaacaggaa
     1261 cggcggcagg gacgccagca gcatggccgg ccactcggca cggatgcgcg acccgagcgc
     1321 gacgccgatc accagcagca cgaccagacc gccgccgaca tgaagccaga aattcgggtc
     1381 gatcatgttc tgacagatgc tggtgcgcac gccgcggctg ccgctcgcgg cgaagtagat
     1441 cccccagtcg acgaagcggt gcgccgcgaa cttggcgcgc accgtgtcga ttgcggtctg
     1501 ggtgtcgaca tgcggcagcc aggccatgcc ggcatagagg gcgagcgcga cggcgaacgc
     1561 cagaccggcc caggccttgt cggtggcgcg ccaggccgac cagccgccgc ggcgaagcag
     1621 cagcgcagcc agcagcggca acccgaagat gaagctcgtc tcgtggatga acagcccgat
     1681 gcagaccagc gcgcaggcga gcatcagcct gcccgcggtc atggcgagtg tcgcgaacgc
     1741 cagcagcgcg gcgatgctca tgtcggtgcg accgacggca tcctcggccc agcgcagcat
     1801 gatggtcacg gccgtgaacg caaagacggc ctggatccac ggcggccccg cgatgcgccg
     1861 gaacagcagg cccgcggcga cggcgaccgc ggcggccgac agcacgtgga acagcagcgt
     1921 ggcgatcagc atgtcggggt tcagcagatg gacgatgctg ccgcccaggc cgcggcggac
     1981 cgcgccgagc tcgaagtagt tgaaccccag ggtcgcggcc tgatagagca ggtacgagtc
     2041 cgtcgtgaag agttcgctgc ggccgccagc gctcgcgatc cagcgcatcg atgcgacgac
     2101 cagcatcagg gccagcagca gggcgggccg cgttctcggg tcgcgcagcg ttggcggttt
     2161 catcgggctc ggtgtcgggg cgggcaaagc cccggtgtta taaccggccc ccgggtgctg
     2221 gagcacttct ggcgagggta ccgatggtcg atccatgaag cgcagcgcgg tgtccgccgc
     2281 cggtctggtc gccgcgggtt tgctgctcgc gctgctggtg gtcaacccgc tgcgcgagct
     2341 gctgacgcaa gacgacggtt gggcctacgc gcgcatggtc gagcgtttgc tgaagaccgg
     2401 cgaataccgg ctcgacgcct ggtcggcggc gaacatgccg gtccagatct acctcgccgc
     2461 cgggctcgcc gaagtgttcg gctactcgtt gagcctgctg cgctgcacga ccttgctgct
     2521 gctggccgtc ggtctggcca gcctgtacgc gttgctgcgc gagctcggca ccgatcgcgg
     2581 gcacgccgca ctgctgacgc tgggcctgct ggccagcccg ctggtgctga tgctcgcgtt
     2641 caccttcatg tccgacgccc agttcctcgg ctggatgttg ctggcgctct ggctgtatgt
     2701 gcgcggcctg cgggtcgaga gcgatgcgct cgtgttctgc ggctcgctcg cggccgcctg
     2761 cgcgatcgga acccgtcagt tcggcatcgc gatcatggcg ggcctggcgc tgacctggct
     2821 ggtctcgaat cgccagcgcc gcccgccgat gcaccgtttg ctgatcgcat tggccgttcc
     2881 gatgctcgtc ggcgtctggc agctgcaggc cggcctgcgc gagcccaatt tcacgcaggc
     2941 gtatcgcgtc gcggatcagc ggcttttcct cgctcactcg gtcgcgacgc tcgcccgcga
     3001 agggctctgg cggagcgtga ccctggtgca ctacctgggc ctgtcgctgc tgccgctggc
     3061 accgcttctt gccggcctgg cgctgcgccg aggcgcgccg cgtgccgccg gcgcgaggtt
     3121 tcagcgctgg cccgtcgtgc tggcgacgct gctcgtggcc gccggtctgg tgtgggcttc
     3181 ggtggcgggg tcgccgctga cggcgcgccc cgccgatccg ggcgaaaccg acccgccccc
     3241 gctgcccctg tactggatgc tgccgaccgc gttcgcggcc cgccatcgcc ggatgacgct
     3301 gctgctcgac gcctgcggtg gcgtcctcgc gctgctgttc gcgtgggtcg tgctgcaggc
     3361 ctggtgggcg cgccggccgg actttcgcgc gctgcgccac gggccgccgg gggtctcgtt
     3421 gctcctcggc accggtctgt cgctgctggt cttgcaactg agctatgtgc agttcaacga
     3481 cacctacctc gccggcctgt tgcccttcgc gctgctgctg ctggtgccgg cactgcgcgc
     3541 aacacccccg cgcggctggg cggcagcgac cggtgcgtgc gcgtatgccg cgatcctggt
     3601 cacgtccttg tggatgcgcg gcgactacaa ccgccagcaa gcctactggg acggggccga
     3661 gcggcttctg cgcagcggcc acgccccgcg cgacatcggc ggcgaactgc actgggcgga
     3721 ataccacggc gcgttcgacg aatggatcgc ggccggtgcg cccggcctcg agcgcgcggg
     3781 cgagggctca tccaaggcgg tctacggccc actgcacgac ccgttctacc gctgggtcga
     3841 gtttcgcagc tggcgggccg gctaccggct gcaggaggaa tcgcaggggc cgccgcccgc
     3901 cggctggcag ctcgtcgaca cggtgcctta ccgcgatggc tacttcgccg ctcgcagcat
     3961 ctggatcctg gcgcgccgcg cggcgcgcga ttgagagtcg ttgcatgtgc cgagttgggg
     4021 atttggcgat gcgaggaaac ccgtagactc tgcacgagtc gcccccggtc ggggcgccga
     4081 aggccccgcc cctgtgacca acgcgaagaa tcgagcccag ggcctcccgt cgtcgccccg
     4141 tatcgccgtc atcggaggcg ggcccatggg cttggcggcg gcgctggaat tggccaagaa
     4201 gggcgcgcgg gtcgaggttc tcgaacgtga cgaccggctc ggcggcatgt cggcccactt
     4261 cgatttctcc ggcacccgca tcgagcgcta ctaccacttc atttgcgccc cggaccagac
     4321 gacgttccag tacctgcgcg aactcgatct cgaagacaag ctgcgctgga ccgatacgcg
     4381 gatgggtttt tacctgaacg ggcaactgca cgactggggc aacccgcagg cgatgctgcg
     4441 tttccccggg ttgtcgctgc tgcagaagct gcgctacggg ctgcacgtga tgcgtgccaa
     4501 gagcatcacc gattggcgcc cgtacgacac gctgacctcc accgagtggg tccagaagtg
     4561 gatcggcaag ccggcctacg aggtgatgtg gcgcagcctg ttccactaca agttctacga
     4621 gttccaggac agcttgtcag ccgcctggct cggcacgcgg atcaagcgcg tggcgctgtc
     4681 gcgcaagagc atctttcagg agcggctggg ctacctggac ggtggctccg aagtgctgct
     4741 cgaagcgatc gcggcgcgcc tgacgcagtg cggtggcagc ttgtcgctcg ccagccccgt
     4801 cgaagcggtg ctgaccgaag gtgcgggcag cacgcggcgc gtgcgcggta ttcttgccgg
     4861 tggcgtggaa cttgccttcg acgccgtcgt gtccaccgcg ccgctgccgc acgttccggc
     4921 gctggtgccc gacctgcccg gcgacgagcg ggccaagatc gatgcgatcc gcaatgtcgg
     4981 cgtggtgtgc gtgatcctga aactgaagcg aagcttcacg ccgaatttct ggctcaacat
     5041 caacgacccg cgggtgcaga tccccgggct gatcgagtac acgaacctga atccgctgga
     5101 cggaagctcg atcgtgtatg ccccgttcta catgccgcac agccatccca agtaccagcg
     5161 gacgaatcag gagttcatcg acgagacgct cgcctgcctg tcggtgatcc gtccggattt
     5221 cgatcgcgac gacgtgctgg ctgccacggc ttcgcgctac cggttcgcgc agaccgtgtg
     5281 ttcgccgggc tttctggagg cgttgccgcc gatgcgcagc gggttgaaag gtttcttcat
     5341 ggccgatacg tcgtactact acccggagga ccgatcgatc tcggaaagcc tgcgcaccgg
     5401 tgcacgactc gcgcacaccg tctgggaggc gctctgacgc cgcaaccgac gacggtgccg
     5461 gcggcgtacg ccgccgaggt gccggcgccg acactggtgg tcgatctcga cggcaccctg
     5521 atccgttccg acctgctgca cgacgccgtg gtccagctgg tgttgcagaa gccctctacg
     5581 ctctggtcga tgccgggctg gctgctgcgc ggcaaggccc acttcaaggc gcaggtggcg
     5641 tcgcgcaccc ggctggaggt cgacggcctg ccctatgaac cggccttggt cgaatggatc
     5701 cgaagcgagc gcgcgagcgg ccgttcggtg attctttgca ccgcgtcgga cgtgcgccag
     5761 gcgcatctcg tcgcgcgggc caccgacttg ttcgatgacg tcatggccag cgacggcacg
     5821 accaacctgt cggcgcagcg caaggccgat gcgctggtgc agcgcttcgg ctggcagggc
     5881 ttcgactatg ccggcaattc gcgcgccgac gttccggtgt ggaaggcttc gcgccgtgcg
     5941 atcgtcgtcg gcgcgacagc gtcgactcga gccgccgcag ccgaggtcgc ggtggtcgag
     6001 cgcgaattcg cgccgaggac cgggcgcctg cgcgcctggt tctcggccct gcgcgtgcag
     6061 cagtgggtga agaacctgct ggtattcgtg ccgcttctcg gcgcccacga attccagaac
     6121 ctgccggcgc tgctgtcggt ggctttggcg ttcgtcgcgt tctccttgtg cgcgtcgtcc
     6181 gtctacctga tcaacgacat cgtcgacctc aacagcgacc gcgggcaccc gcgcaagcgc
     6241 ggccggccgt tcgcgtccgg cgcgctgccg atcccgcacg gcatcgtcgt cagcctgctg
     6301 ctgatggcga cggcgttcgc cctggcgctc gcgagcgtca acctcgagtt cgtcgcctgg
     6361 ctggcagcct acctcgtgac gacggcctgc tactcgctgt ggttgaagcg caagatcctg
     6421 gtcgattcgc tggtgctggc ggcgctgtac acattgcggc tgctcgccgg cggcgcggcg
     6481 gcgggcaccg ccaccgggtt ctggttgctg gcgttctcgt tgttcctgtt cctgagcctg
     6541 gccttggtca agcgctattc ggaactgaga gagacgttca ggcgcggccg cgcgcgcacc
     6601 cccgggcgcg actacatcat cgaagacctg ccgctggtgc agatgttggg catcgcgtcg
     6661 ggcttcagct ccgtcatggt gctggcgctc tacatcaatg gcgacacggt cgcgcgcctg
     6721 taccctcacg aacagatgat ctggttcacc gtgccggtgc tgctgtactg ggtgagccga
     6781 ctctggttga aggcgcaccg cggcgaactc gacgacgacc cgctggtctt cgcgttgagc
     6841 gacaacctga gccgcgtcac catcctgatg ttcttcggct cgctgctgct cgccagccgg
     6901 cactgatgcg cgcgccgctc gcctcctggg ggtgcctgag ccggcgcgaa cacgtcgtgg
     6961 tgccgctgcg cagccgggcc cgcgcgccgg ccgcggtgag agcgtccgcg ttgccggcga
     7021 tcgcgcatgg caacgggcgc agctatggcg acgaggcgct caacggcggc ggcacggtct
     7081 ggctcgcgcg cgggctcgac aagttcatcg gcttcgacgc ggaccgcggc gtgttgcgct
     7141 gcgaggccgg ggtcagcttg cgggagatca tcgacgtcgt gctgccgcgc ggctggttcc
     7201 tgcccgtcac ccccggcacg cagttcgtca cgctcggcgg cgcggtcgcc aacgacgtgc
     7261 acggcaagaa ccaccatcgc tgcgggacgc tgggcgaaca cgtcacggcg ctgacgctgt
     7321 tgcgcagcga cggccaggtc atcgactgcg ggccggcggc gcggtccgac tggttctgcg
     7381 cgaccgtggg cggcatgggc ctgaccggtg tcattctcga tgtcacgctc cagctgcgtc
     7441 gcgtgcccgg cccctggatc gagacccgca cacacgcctt ccaga
//
LOCUS       DATMJB010000512         4611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_318163, whole genome shotgun sequence.
ACCESSION   DATMJB010000512 DATMJB010000000
VERSION     DATMJB010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318163"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VNU48_11030"
     CDS             <1..297
                     /locus_tag="VNU48_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HWI11854.1"
                     /translation="PDYLLKVRGMSMRDAGIMDGDLLAVQKATDAKNGQIVVARLGDE
                     VTVKRFRRTRHGIELIPENPDFQTIVVPTGGDAGELQFELEGLAVGLIRNNMML"
     gene            297..605
                     /locus_tag="VNU48_11035"
     CDS             297..605
                     /locus_tag="VNU48_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWI11855.1"
                     /translation="MGATVIKAPKAACAADCAALEQLPNIGPSLAADLRLIGIEHPAD
                     LRGKDAYQLYQRLCAATGQRQDPCVLDTFMAATDFMRGAPAAPWWHYTAQRKALFGQV
                     "
     gene            695..904
                     /locus_tag="VNU48_11040"
     CDS             695..904
                     /locus_tag="VNU48_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11856.1"
                     /translation="MSTRFYAGPISIGMGRRWDDKFEPERGAESKPRPVRPPFGRMTA
                     SMVPPSAPSSTDSLAPRTDPIAPQR"
     gene            complement(938..1450)
                     /locus_tag="VNU48_11045"
     CDS             complement(938..1450)
                     /locus_tag="VNU48_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HWI11857.1"
                     /translation="MSITMYSASVPIFLRLLENCGHWLEKAEAHAQAKEFDSVVYLST
                     RLAPDMLPFTSQIQVACDGAKFAIARLAEVEAPKFDDTEATLADLRERLRATSAFIAS
                     VPAAKLVGTEDKDVTIPRRTGPLTLKGEAYLKHFALPNFFFHMTTAYALLRHNGVELG
                     KMDYLGAIQQ"
     gene            1636..2643
                     /locus_tag="VNU48_11050"
     CDS             1636..2643
                     /locus_tag="VNU48_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HWI11858.1"
                     /translation="MNIIILDDYQDAVRKLKCASVMEQLNAKVFTNTVKGIGQLSVRL
                     RDAEVLVPIRERTQFPKQLLEKLPKLKLISQTGKAGPHIDVDTCTRMGIAVAEGVGSP
                     VAPAELTWALIMAAMRRLPQYVGNLKHGAWQQSGLKASSMPANFGVGMVLKGKTLGIW
                     GYGKIGQMVAGYGKAFGMHITVWGSESAREHAAKDGCVAAPSCEAFFEACDVLSLHLR
                     LHESTRGIVRHDALARMKPTALLVNTSRAELIEEGALVSALNRGRPGMAAVDVFESEP
                     ILQGHPLLRLENAVCTPHIGYVEQDSYELYFRAAFDNVVNFINGTPTNILNPEALRVL
                     R"
     gene            2640..3806
                     /locus_tag="VNU48_11055"
     CDS             2640..3806
                     /locus_tag="VNU48_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI11859.1"
                     /translation="MTAAAPLRGSVRWALMFGNFVIGCGVMSTAGTLNDIARSLEVSV
                     ALAGQLIAIAAATMAFGAPLLAGWVAGFDRRRLLALALFWYGAGHVLSALMPSYAALW
                     PVRAATMLAAAVFTPQAAAAIGFMAPVHERGRAITFIFLGWSVASVVGVPMSAWIGET
                     FGWGVAFDTIGVLALLAAVWVYAVMPDGVRQPALSLRAWKGVLTNPVPMAMVLVTALS
                     GAGQFTLLSYLAPYYKQALRANTEQITLLLLWFGAFGLVGNVVLTHVIDRVGPNRAVA
                     VTLSLIALSLLAWPLGTSFASMAVVLVPWALGCFSSNSTQQARLSIAAPALAPALMAL
                     NTSAIYLGQAAGAASGGWLIAHGGYGPLNWCGLAWLLVAIALSLWAGRRVAVAA"
     gene            3803..4378
                     /locus_tag="VNU48_11060"
     CDS             3803..4378
                     /locus_tag="VNU48_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HWI11860.1"
                     /translation="MTAPLGRAGAAPASLRELFLAFNRLALQGFGGVLPVAQRELVER
                     HRWLTKDEFVEMLAISQVMPGPNVVNLALIFGDRTFGTRGALVALGGMLLAPLAVVLA
                     LTVLYVQFDRYPAVTGALRGMGAVAAGLILSTGLKLLVTLRRNVMGLPLCLGLAALTA
                     LGTAWLRLPLVWVIAGLGSIAIAIAWQRLRP"
     gene            4375..>4611
                     /locus_tag="VNU48_11065"
     CDS             4375..>4611
                     /locus_tag="VNU48_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HWI11861.1"
                     /translation="MNHDAADLLGLFLHFLVLSLLAVGGAITTVPDMHRYIVAEHHWL
                     TDAQFTASIAIAQAAPGPNVLFVAVLGWNVAGPLG"
BASE COUNT          655 a         1615 c         1597 g          744 t
ORIGIN      
        1 cccgactacc tgctgaaggt gcgcggcatg agcatgcgcg acgccggcat catggacggc
       61 gacctgctcg cggtgcaaaa agccaccgac gcgaagaacg gccagatcgt cgtcgcgcgg
      121 ctcggcgacg aggtcaccgt gaagcgcttc cgccgcaccc gccacggcat cgagctgatc
      181 cccgagaacc ccgacttcca gaccatcgtc gtgcccaccg gcggcgatgc cggcgagttg
      241 cagttcgagc tcgaaggact ggccgtcggg ctgatccgca acaacatgat gctgtgatgg
      301 gtgcgaccgt catcaaggca ccgaaggccg cgtgcgcggc cgactgcgcg gcactcgagc
      361 agctgccgaa catcggcccg tcgctggccg ccgacctgcg cctgatcggc atcgagcacc
      421 cggccgacct gcgcggcaag gacgcttatc agctgtatca gcgcctgtgc gccgccaccg
      481 gccagcgcca ggacccgtgc gtgctcgaca ccttcatggc cgcaaccgac ttcatgcgcg
      541 gcgcgcccgc cgcgccgtgg tggcactaca ccgcgcagcg caaggccttg ttcgggcagg
      601 tctgacgcgc cttcgccgag gcgcggatct ggcggccaga accggctcat ttcggcgtct
      661 cggccgatcg gccggtgcgg aacaatcgca cctcatgagc acccgcttct acgctgggcc
      721 gatcagcatc ggcatgggtc gccgctggga cgacaagttc gaacccgaac gcggcgccga
      781 atcgaagccg cgaccggtgc gtccgccctt cgggcgcatg accgcgtcga tggtgccgcc
      841 gtccgcgccg tcgtcaaccg actcgctcgc gccgcgaacc gatccgatcg ccccgcagcg
      901 ctgaagagcc cgagaggctt tcgttcacac ccgcttctta ttgctggatc gcgccgagat
      961 agtccatctt gccgagctcg acgccgttgt gacgcaacag cgcgtaggcg gtcgtcatgt
     1021 ggaagaagaa gttcggcagc gcgaagtgct tcaggtaggc ctcgcccttc agcgtcagcg
     1081 ggccggtgcg gcgcgggatg gtcacgtcct tgtcttcggt gcccaccagt ttggccgcgg
     1141 gcaccgacgc gatgaacgcg ctggtcgcgc gcaagcgttc gcgcaagtcg gccagcgtcg
     1201 cttcggtgtc gtcgaatttc ggcgcctcga cttcggcgag gcgcgcgatc gcgaacttcg
     1261 cgccgtcgca ggcaacctgg atctggctcg tgaacggcag catgtcgggc gcaagccgcg
     1321 tgctcaggta caccaccgag tcgaactcct tcgcctgcgc atgcgcctcg gccttctcca
     1381 gccagtgacc gcagttctcg agcaggcgaa gaaagatcgg cacgctggcc gagtacatgg
     1441 tgatggacat gaggtcgtct ccgggaatcg ttgtgcgaaa ccggccatcg taccggcgtt
     1501 cgccggggcg tgcacaatca ccgcgacctc tcgagcaaat gtggctctgc cgctttgctc
     1561 gaacccccac gggggcgggc tgggcgcagc ccggacctgg gggcccctca gaacccgcga
     1621 gacgacccga ttcccgtgaa catcatcatc ctcgacgact accaagacgc ggtgcgcaag
     1681 ctcaagtgcg caagcgtgat ggagcagctc aacgccaagg tcttcacgaa caccgtcaag
     1741 ggcatcggcc agctgtcggt gcgactgcgc gatgccgaag tgctggtgcc gatccgcgaa
     1801 cgcacccagt tccccaagca gctgctcgaa aagctcccca agctcaagct gatctcgcag
     1861 accggcaagg ccggcccgca catcgatgtc gacacctgca cgcgcatggg catcgcggtg
     1921 gccgagggcg tgggctcgcc ggtcgcgccg gccgagctga cctgggcgct gatcatggcc
     1981 gcgatgcgac gcctgccgca gtacgtcggc aacctcaagc acggcgcctg gcagcagtcg
     2041 gggctgaagg catcgtcgat gccggcgaac ttcggcgtcg gcatggtgct gaagggcaag
     2101 acgctgggga tctggggcta cggcaagatc ggccagatgg tcgccggcta cggcaaggcc
     2161 ttcggcatgc acatcacggt atggggcagc gaatcggcac gcgagcacgc ggccaaggac
     2221 ggctgcgtcg ccgcgccgag ctgcgaagcc ttcttcgagg cctgcgacgt gctctcgctg
     2281 cacctgcggc tgcacgagag cacgcgcggc atcgtcaggc acgacgcgct ggcgcgcatg
     2341 aagccgaccg cactgctcgt caacacctcg cgcgccgagc tgatcgagga aggcgcgctg
     2401 gtctcggcgc tgaaccgcgg ccgccccggc atggccgcgg tcgacgtgtt cgagagcgaa
     2461 ccgatcctgc agggccaccc gctgctgcgg ctggagaacg cggtgtgcac gccgcacatc
     2521 ggctacgtcg agcaggacag ctacgagctg tactttcgcg ccgcgttcga caacgtggtg
     2581 aacttcatca acggcacgcc gacgaacatc ctgaaccccg aggccctgcg agtgcttcga
     2641 tgaccgcagc cgcgccgctg cgcggcagcg tccgctgggc gctgatgttc ggcaacttcg
     2701 tgatcggctg cggcgtgatg tcgacggccg gcacgctcaa cgacatcgcg cgctcgctcg
     2761 aggtctcggt cgcgctggcc ggccagctga tcgcgatcgc cgccgcgacg atggccttcg
     2821 gcgcgccgct gctcgccggc tgggtcgccg ggttcgaccg ccgccggctg ctcgcgctgg
     2881 cgctgttctg gtatggcgcc ggccacgtgc tcagtgcgct gatgccgagc tacgcggcgc
     2941 tgtggccggt gcgcgccgcg acgatgctgg ccgcggcggt gttcacgccg caggccgccg
     3001 ccgcgatcgg cttcatggcg ccggtgcacg aacgcgggcg cgcgatcacc ttcatcttcc
     3061 tcggctggtc ggtggcctcg gtggtcggcg tgccgatgtc ggcctggatc ggcgagacct
     3121 tcggctgggg cgttgcgttc gacacgatcg gcgtgctggc gctgctggcc gccgtctggg
     3181 tctacgcggt gatgccggac ggcgtgcgcc agcccgcgct ctcgctgcgc gcctggaagg
     3241 gcgtgctgac gaatccggtg ccgatggcga tggtgctggt caccgcgctg tcgggcgccg
     3301 ggcagttcac gctgctctcg tatctcgcgc cgtactacaa acaagcgctg cgcgcgaaca
     3361 ccgagcagat cacgctgctg ctgctgtggt tcggcgcctt cggcctggtc ggcaacgtgg
     3421 tgctgacgca cgtgatcgac cgggtcggcc cgaaccgggc cgtcgcggtg accttgtcgc
     3481 tgatcgcgct gtcgctgctg gcctggccgc tcggcacctc gttcgccagc atggcggtcg
     3541 tgctggtgcc gtgggcgctg ggctgcttct cgtcgaactc gacccagcag gcgcgcctga
     3601 gcattgccgc gcccgcgctc gcgccggcgc tgatggcgct gaacacctcg gcgatctacc
     3661 tcggccaggc ggccggcgcc gcgagcggcg gctggctgat cgcgcacggc ggctacggcc
     3721 cgctgaactg gtgcggcctg gcgtggctgc tggtggcgat cgcgctcagc ctgtgggccg
     3781 ggcggcgcgt ggcagtggcc gcatgacggc gcctctgggt cgcgccggcg ctgcaccggc
     3841 ctcgctgcgc gagctgttcc tcgccttcaa tcggctcgca ctgcagggct tcggcggcgt
     3901 gctgccggtc gcgcagcgcg agctggtcga acgccatcgc tggctcacca aggacgagtt
     3961 cgtcgagatg ctggccatca gccaggtgat gccgggcccg aacgtcgtga acctcgcgct
     4021 gatcttcggc gaccgcacgt tcggcacgcg cggcgcgctg gtcgccctgg gcggcatgct
     4081 gctcgcgccg ctcgccgtcg tgctcgcgct gaccgtgctc tacgtccagt tcgaccgcta
     4141 cccggcggtc accggcgcgc tgcgcgggat gggcgcggtg gcggccgggc tgatcctgtc
     4201 gaccggcctg aagctgctgg tcacgctgcg ccgcaacgtg atggggctgc cgctgtgcct
     4261 cggcctggcc gcattgaccg cgctcggcac ggcctggctg cggctgccgc tggtgtgggt
     4321 catcgccggc ctcggttcga tcgcgatcgc gatcgcctgg cagcggttgc gaccatgaac
     4381 cacgacgcgg ccgacctgct cggcctgttc ctgcacttcc tcgtgctcag cctgctcgcg
     4441 gtcggcggcg cgatcacgac cgtgcccgac atgcaccgct acatcgtcgc cgagcatcac
     4501 tggctgaccg acgcgcagtt caccgcgtcg atcgcgatcg cgcaggccgc gcccggcccg
     4561 aacgtgctgt tcgtcgcggt gctgggctgg aacgtcgcag gcccgctcgg c
//
LOCUS       DATMJB010000513         3909 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_53654, whole genome shotgun sequence.
ACCESSION   DATMJB010000513 DATMJB010000000
VERSION     DATMJB010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53654"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VNU48_11070"
     CDS             <1..508
                     /locus_tag="VNU48_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HWI11862.1"
                     /translation="IKANIPTRLSFQVSSKIDSRTILDQMGAEALLGMGDMLYMPSGT
                     GFPVRVHGAFVSDDEVHRVVEYLKSQGAPNYIEGILEGGVLDDGPDVLTGEGGVMGGG
                     ESDAMYDQAVAIVLQHKRASISLVQRHLRIGYNRAARLLEQMEKSGLVSSMSTNGNRD
                     IIVPNRAE"
     gene            505..1137
                     /gene="lolA"
                     /locus_tag="VNU48_11075"
     CDS             505..1137
                     /gene="lolA"
                     /locus_tag="VNU48_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HWI11863.1"
                     /translation="MKRALATLGAAFALSLAAFAAHADSVDTLREFIRDVKTGRAQFT
                     QTVTSPDGAKKKTSSGSFEFARPNRFRFAYTKPFEQVIVADGQKVWIFDADLNQASSR
                     KFTSALGATPAALLAGGSLDKDFDLTPVPARDAAKDGLDWVQALPKAKDGAFKSVRVG
                     FRGKELAAVEIVDAFDQRSLLQFSQFAAGVPIAANAFQFTPPPGADVIEQ"
     gene            1134..2441
                     /locus_tag="VNU48_11080"
     CDS             1134..2441
                     /locus_tag="VNU48_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HWI11864.1"
                     /translation="MSAEPQLFDEAPDAPLAERLRPKTLDEVIGQLQLLGPGKPLRVA
                     FESGKLHSMILWGPPGVGKTTLARLMAQGFDALFIAISAVLGGVKDIRDAVERAQIAR
                     HGGSRTVVFVDEVHRFNKAQQDAFLPHVESGLFTFIGATTENPSFEVNSALLSRAVVH
                     VLKPLGDADLRELLQRAQALLAAPPISEPARERLIGYADGDARRLLNAYENLMGTAAG
                     VAEIDEALLERSLGEQLRRYDKGGEQFYDTISALHKAVRGSDPDASLYWFVRMLDGGV
                     DPRYAARRLVRMASEDIGLADPRALRLTLDAAETYERLGSPEGELALAQAVVYLAVAP
                     KSNALYVAYQAARAFVQQDGTRAVPLRLRNAPTALMKGLDYGRGYRYAHDEEGGFAAG
                     ESYWPDDMNAPQFYAPVDRGLEIRIGEKLQELRRLNEAAAKPR"
     gene            2677..3600
                     /locus_tag="VNU48_11085"
     CDS             2677..3600
                     /locus_tag="VNU48_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWI11865.1"
                     /translation="MDTFIQQIINGLVLGSVYALVALGYTMVYGIINLINFAHGEVLM
                     VGALTSWTVVTAMAGLGLPGWLLLLISLIAAIVVCCALNYSIEKIAYRPLRNAPRLAP
                     LITAMGMSLLLQTLAMIIWKPNYKSYPILLPGDPHMIGGAVINNVQIMILVVTAIVLA
                     GLMYLVNKTKLGRAMRATAENPRVAGLMGVKPDSVISATFIIGAALAALAGVMYAANY
                     GSVQHTMGFLPGLKAFTAAVFGGIGNLPGAMVGGVLLGLIESFGAGYIGAITGGVLGS
                     NYQDIFAFIVLILVLTLRPQGLLGERVADRA"
     gene            3614..>3909
                     /locus_tag="VNU48_11090"
     CDS             3614..>3909
                     /locus_tag="VNU48_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI11866.1"
                     /translation="MQKNNIIKFVVLAIGLLILPLIAQQAGNAWVRIIDIALLYVLLA
                     LGLNIVVGFAGLLDLGYVAFYAVGAYMFGLLASSQLTDTFPAIAAMFPNGLHTP"
BASE COUNT          619 a         1329 c         1340 g          621 t
ORIGIN      
        1 gatcaaggcg aacatcccga cacggctgag ctttcaggtc agcagcaaga tcgattcgcg
       61 caccatcctc gaccagatgg gcgccgaggc gctgctgggc atgggcgaca tgctgtacat
      121 gccctcgggc accggtttcc cggtgcgggt gcacggcgcc ttcgtcagcg acgacgaggt
      181 gcaccgcgtg gtcgagtacc tgaagagcca gggcgcgccg aactacatcg agggcattct
      241 cgaaggcggc gtgctcgacg acggccccga cgtgctgacc ggcgagggag gcgtgatggg
      301 tggcggcgag agcgacgcga tgtacgacca ggcggtggcg atcgtgctgc agcacaagcg
      361 cgcgtcgatc tcgctggtgc agcggcatct gcgcatcggc tacaaccgcg ccgcgcggct
      421 gctggaacaa atggagaaat ccgggctcgt atcgagcatg tccaccaacg gcaatcgcga
      481 catcatcgtg ccgaaccgcg ccgaatgaaa cgtgctctgg ccactctggg tgcagcgttc
      541 gcgctgagcc tcgcggcgtt cgccgcccac gccgattcgg tcgacacctt gcgcgagttc
      601 atccgcgacg tgaagaccgg ccgcgcgcag ttcacgcaga cggtcacctc gcccgacggc
      661 gcgaagaaga agacctccag cggcagcttc gagttcgcgc ggccgaaccg cttccgcttc
      721 gcatacacca agcccttcga gcaggtcatc gtcgccgacg gccagaaggt ctggatcttc
      781 gacgccgacc tgaaccaggc gagctcgcgc aagttcacgt cggcgctggg cgcgacgccg
      841 gccgcgctgc tcgccggcgg ctcgctcgac aaggatttcg atctcacgcc cgtgcctgca
      901 agagacgccg cgaaggacgg cctcgattgg gtgcaggcgc tgccgaaggc caaggacggc
      961 gcgttcaagt cggtgcgcgt gggctttcgc ggcaaggaac tggcggcggt cgagatcgtc
     1021 gacgccttcg accagcgctc gctgctgcag ttcagccagt tcgcggccgg cgttccgatc
     1081 gccgcgaacg cgttccagtt cacgccgccg cccggcgccg atgtgatcga gcagtgagcg
     1141 ccgagccgca acttttcgac gaggcgccgg acgcgccgct cgccgaacgc ttgcgcccga
     1201 agacgctcga cgaggtgatc ggccaactcc agctgctcgg gcccggcaag ccgctgcgcg
     1261 tcgcgttcga gtcgggcaag ttgcattcga tgatcctgtg gggaccaccc ggtgtcggca
     1321 agaccacgtt ggcgcgcctg atggcgcagg gtttcgacgc gctgttcatc gcgatctcgg
     1381 cggtgctcgg gggcgtcaag gacattcgcg acgcggtcga gcgtgcccag atcgcgcgcc
     1441 acggcggcag ccgcacggtc gtcttcgtcg acgaggtgca ccgcttcaac aaggcgcagc
     1501 aggacgcatt cctgccgcac gtcgaatcgg gcttgttcac cttcatcggc gcgaccaccg
     1561 agaacccgtc gttcgaggtc aactccgcgc tgctgtcgcg cgccgtcgtg catgtgctca
     1621 agccgctggg cgacgcggac ctgcgcgagc tgctgcagcg cgcgcaggcg ctgctggcgg
     1681 cgccgccgat cagcgagccg gcgcgcgagc gcctgatcgg ctacgccgac ggcgacgcgc
     1741 ggcgcctgct caacgcctac gagaacctga tgggcacggc cgctggcgtg gccgagatcg
     1801 acgaggcgct gctcgagcgc tcgctgggcg agcagctgcg ccgctacgac aagggcggcg
     1861 agcagttcta cgacacgatc tctgcgctgc acaaggcggt gcgcggcagc gaccccgacg
     1921 cgtcgctgta ctggttcgtg cgcatgctcg acggcggcgt cgatccgcgc tacgcggcgc
     1981 gccggctggt gcgcatggcc agcgaggaca tcggcctggc cgacccgcgc gcgctgcgcc
     2041 tgacgctcga cgcggccgag acctacgagc gcctcggttc gcccgagggc gaactcgcac
     2101 tcgcgcaggc ggtcgtctac ctcgcggtcg cgccgaagag caacgcgctt tacgtggcct
     2161 accaggcggc gcgcgcgttc gtccagcagg acggcacccg cgcggtgccg ctgcgcctgc
     2221 gcaacgcgcc gacggcgctg atgaagggcc tggactacgg ccgcggctac cgctacgcgc
     2281 acgacgagga aggcggcttc gccgccggcg agagctactg gcccgatgac atgaacgcgc
     2341 cgcagttcta cgcgccggtc gatcgcggcc tggaaatccg catcggtgaa aagctgcagg
     2401 agttgcgccg cctcaacgag gccgccgcca agccccgctg agccgggcgc ccgcgcgcgg
     2461 gtcgctgaca gcatttcgcg ccgctgtcat cgggaaagtc agaggcgggg ccgcggatgc
     2521 gcgctcctag aatccgccac cgtccgggcc gccttcgcgt tggacgcgac ccggcaacgc
     2581 aggtcgtggc cgggaaattg ctactgggtg agggtttggg accagaccgc gcgctggacg
     2641 cggcggccgg agtcgacaac gcaggagaga acccgcatgg acaccttcat ccagcagatc
     2701 atcaacggcc tcgtgctcgg cagcgtgtat gcgctggtcg cgctgggcta cacgatggtc
     2761 tacggcatca tcaacctgat caacttcgcg cacggcgagg tgctgatggt gggcgcgctg
     2821 acgagctgga cggtcgtcac cgcgatggct ggcctcggcc tgccgggctg gctgctgctg
     2881 ctgatctcgc tgatcgccgc gatcgtcgtc tgctgtgcgc tcaactacag catcgagaag
     2941 atcgcctacc ggccgctgcg caatgcgccg cgcctggcac cgctgatcac cgcgatgggc
     3001 atgagcctgc tgctgcagac cctggcgatg atcatctgga agccgaacta caagtcgtat
     3061 ccgatcctat tgccgggtga cccgcacatg atcggcggcg cggtcatcaa caacgtgcag
     3121 atcatgatcc tggtcgtcac cgcgatcgtg ctggccgggc tcatgtacct ggtcaacaag
     3181 accaagctcg gccgcgcgat gcgcgccacc gccgagaacc cgcgcgtggc cgggcttatg
     3241 ggcgtgaagc ccgactcggt gatctcggcc accttcatca tcggtgccgc gctggccgcg
     3301 ctggccggcg tgatgtacgc ggccaactac ggctcggtgc agcacacgat gggtttcctg
     3361 ccgggcctga aggccttcac cgcggcggtg ttcggcggca tcggcaacct gcccggcgcg
     3421 atggtcggcg gcgtgctgct ggggctgatc gagtcgttcg gcgccggcta catcggcgcc
     3481 atcaccggcg gtgtgctggg cagcaactac caggacatct tcgcgttcat cgtgctcatc
     3541 ctcgtgctga ccttgcgccc gcaaggcctg ctcggcgagc gtgtggcgga ccgggcctga
     3601 acgggagcac accatgcaaa agaacaacat catcaagttc gtggtgctcg cgatcgggct
     3661 gctgatcctg ccgctgatcg cgcagcaggc cggcaacgcg tgggtccgca tcatcgacat
     3721 cgcgctgctc tacgtgctgc tggcgctggg cctgaacatc gtcgtcggct tcgccgggct
     3781 gctcgatctc ggttacgtgg cgttctacgc ggtcggtgcc tacatgttcg ggctgctcgc
     3841 ctcgtcgcag ctgaccgaca ccttcccggc gatcgccgcg atgttcccga acgggctgca
     3901 cacgccgtg
//
LOCUS       DATMJB010000514         2975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_375630, whole genome shotgun sequence.
ACCESSION   DATMJB010000514 DATMJB010000000
VERSION     DATMJB010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375630"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VNU48_11095"
     CDS             <1..209
                     /locus_tag="VNU48_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HWI11867.1"
                     /translation="KPVEGRSVETLTAELVAAAAGASDMVVIVSADATAAHQSVINVM
                     DAARRANLPRLTFATQTSSGGEAH"
     gene            232..1287
                     /gene="lpxK"
                     /locus_tag="VNU48_11100"
     CDS             232..1287
                     /gene="lpxK"
                     /locus_tag="VNU48_11100"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HWI11868.1"
                     /translation="MAHAPTPTWSQRLQQAWLARAPLACAMLPLAAAFGALAALRRGL
                     YRVGLLPTPALPVPVVVVGNLITGGAGKTPTVIAVVGLLRRHGYTPGIVSRGHGGSHA
                     GVLEVTRGLSAAQSGDEPLLMQLRTGAPLVVGRDRVAAARELLGRHPQVDVIVSDDGL
                     QHLALARTAQVIVFDERGAGNGWLLPAGPLRETLPNALPARSLIVYNAAAPSTPLPGT
                     LAKRRLTGVVPLRDWWAGTAASPDALDALRGRPLVAAAGLARPERFFAMLREAGLDFE
                     GLPLPDHHDYAVLPWAAGTADVVLTEKDAIKLDPDLINATRIWVAPLDFELDAAFEAA
                     LLALLPPPGHRHGNSFA"
     gene            1268..1480
                     /locus_tag="VNU48_11105"
     CDS             1268..1480
                     /locus_tag="VNU48_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HWI11869.1"
                     /translation="METRLLNLLVCPICKGPLEHLRPPLQEQVELVCHADGLAFPVRD
                     GIPVMLETEARSLDAARAAEPAAPTA"
     gene            1477..2232
                     /gene="kdsB"
                     /locus_tag="VNU48_11110"
     CDS             1477..2232
                     /gene="kdsB"
                     /locus_tag="VNU48_11110"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HWI11870.1"
                     /translation="MTFTVLVPARLASTRLPGKPLADIAGLPMIVRVARNAARSAASR
                     VVVAADHVTIVDACRAHGVTALLTRADHATGSDRLAEACALLGLDGNDLVVNVQGDEP
                     LLDPQMIDACAALLAQHPDCVMSTAAHAIDARDELDNPNVVKVVLDAAGRALYFSRAP
                     IPCWRDARAHGGAAVLPDPPPLRHIGLYAYQAGFLRRFPLLAASPLEQIEALEQLRVL
                     WHGERIAVHVSASRPGPGVDTPEDLMRVRALFG"
     gene            2390..>2975
                     /gene="adk"
                     /locus_tag="VNU48_11115"
     CDS             2390..>2975
                     /gene="adk"
                     /locus_tag="VNU48_11115"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWI11871.1"
                     /translation="MRLILLGPPGAGKGTQAKFICEKYGIPQISTGDMLRAAVKAGTD
                     MGLAAKKVMDSGGLVSDDIIIGLVKERIAQPDCVNGFLFDGFPRTIVQADAMKTAGVK
                     LDLVLEIDVPDESIIERMSGRRVHMASGRTYHLTFNPPKIDGKDDVTGEELIQRADDE
                     EATVRNRLAVYKDQTRPLIEYYAKWAASGDAGAPR"
BASE COUNT          418 a         1109 c         1032 g          416 t
ORIGIN      
        1 gcaaaccggt cgaggggcgc agcgtcgaga ccctgaccgc cgaactcgtc gcggcagcgg
       61 cgggcgcgag cgacatggtc gtgatcgtgt cggccgacgc caccgccgcg caccagagcg
      121 tgatcaacgt gatggacgcc gcgcgccgcg ccaacctgcc gcgcctgacc ttcgcgaccc
      181 agacctcgtc gggcggcgaa gcgcactgac gcaccgcagc gccgcagcgc gttggcccac
      241 gctcccacac cgacctggtc gcagcggctg cagcaggcct ggcttgcgcg cgctccgctg
      301 gcctgcgcga tgctgccgct ggcggctgcg ttcggcgcgc tggcggcgct gcggcgcggt
      361 ctctaccgcg tcggcctgtt gccgacgcct gcgctgccgg tgccggtcgt cgtcgtcggc
      421 aacctgatca ccggcggcgc cggcaagacg ccgaccgtga tcgccgtcgt gggcctgctg
      481 cggcgccacg gctacacgcc cggcatcgtg tcgcgtggcc atggcgggag tcacgccggc
      541 gtgctcgagg tgacgcgcgg cctgagcgcg gcgcaatccg gcgacgagcc gctgctgatg
      601 cagctgcgca ccggcgcgcc gctggtggtc ggacgcgacc gcgtcgcggc ggcccgcgaa
      661 ctgctgggcc ggcatccgca ggtcgacgtg atcgtcagcg acgacggcct gcagcacctc
      721 gcgctggcac gcaccgcgca ggtcatcgtg ttcgacgagc gcggggccgg caacggctgg
      781 ctgctgcccg ccggcccgtt gcgcgaaacg ttgccgaacg cattgcctgc gcgcagcctg
      841 atcgtctaca acgcggccgc gccctccacg ccgctgcccg gcacgttggc gaagcgccgg
      901 ctcaccggcg tcgtgccgct gcgcgactgg tgggccggta cggccgcctc gcccgatgcg
      961 ctcgatgcac tgcgcggccg gccgttggtg gccgcggcgg ggctggcgcg acccgaacgc
     1021 ttcttcgcga tgctgcgcga ggccggcctg gacttcgagg ggctgccgct gcccgatcac
     1081 cacgactatg ccgtcctgcc ctgggcggcc ggcaccgccg atgtcgtgct caccgagaag
     1141 gacgcgatca agctcgatcc cgacctcatc aatgccacgc gcatctgggt cgcgccgcta
     1201 gacttcgaac tcgacgcggc gttcgaggcc gcgctgctcg ccctgctgcc accgcccgga
     1261 caccgccatg gaaactcgtt tgcttaacct gctcgtctgc ccgatctgca agggcccgct
     1321 cgaacacctg cggccgccgc tgcaggagca ggtcgagctc gtctgccacg cagacgggct
     1381 ggcgttcccg gtgcgcgacg gcatcccggt gatgctggaa accgaagcgc gctcgctcga
     1441 tgcggcccgc gcggccgagc cggccgcccc gaccgcatga ccttcaccgt gctggtgccg
     1501 gcgcgcctcg cgtcgacgcg gctgccgggc aagccgctgg ccgacatcgc gggcctgccg
     1561 atgatcgtgc gcgtggcccg caacgcggcg cgttcggcgg cgagccgcgt ggtcgtcgcg
     1621 gccgaccacg tcacgatcgt cgacgcctgc cgcgcgcacg gcgtcaccgc gctgctgacg
     1681 cgcgccgacc acgccaccgg cagcgaccgg ctcgccgagg cctgcgcgct gctcggcctg
     1741 gacggcaacg atctggtcgt caacgtgcag ggcgacgagc cgctgctgga cccgcagatg
     1801 atcgacgcct gcgcagccct gctggcgcag caccccgact gcgtgatgag caccgcggcg
     1861 catgcgatcg acgcgcgtga cgagctcgac aacccgaacg tcgtcaaggt cgtgctcgac
     1921 gcggccggcc gcgcgctgta cttctcgcgc gcgccgatcc cgtgctggcg cgatgcacgc
     1981 gcgcacggcg gtgcggcagt gctgcccgat ccgccgccgc tgcggcacat cggcctgtat
     2041 gcctaccagg ccggcttcct gcgccgcttc ccgctgctgg ccgcgagccc gctcgagcag
     2101 atcgaggcgc tcgagcagct gcgcgtgttg tggcacggcg agcgcatcgc ggtgcacgtc
     2161 agtgcctcgc gcccggggcc cggggtcgac acgcccgaag acctgatgcg cgtgcgcgcg
     2221 ctgttcggct agtgcaggtc ggatttcggc tcgcgcgcag cgcgtgcctt cctctccctg
     2281 cgcgtcagtt tcgcagcagg gagcgcatgt tattctcgat gcgactcatg cccgccggcg
     2341 cagcatgcgc atcgcggcgc acgcacaaga cacgagaacg aggccatcca tgagactgat
     2401 cctgttgggc ccgcccggcg cgggcaaggg cacccaagcc aaattcatct gcgaaaagta
     2461 cgggatcccg cagatctcga ccggcgacat gctgcgcgcg gccgtcaagg ccggcaccga
     2521 catgggcctg gccgcgaaga aggtgatgga ctcgggcggc ctcgtcagcg acgacatcat
     2581 catcggcctc gtcaaggaac gcatcgcgca acccgactgt gtcaacggct ttttgttcga
     2641 cggctttccg cgcaccatcg tccaggccga cgcgatgaag acggccggcg tcaagctcga
     2701 cctcgtgctc gagatcgacg tgcccgacga gtccatcatc gagcgcatga gcggccgccg
     2761 cgtgcacatg gcctcgggcc gcacctacca cctgacgttc aacccgccca agatcgacgg
     2821 caaggacgac gtgaccggcg aagagctgat ccagcgcgcc gacgacgagg aggcgaccgt
     2881 gcgcaaccgc ctcgcggtct acaaggatca gacgcgcccg ttgatcgagt actacgccaa
     2941 gtgggcggcc agcggcgacg ccggggcccc gcgct
//
LOCUS       DATMJB010000515         2760 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_797676, whole genome shotgun sequence.
ACCESSION   DATMJB010000515 DATMJB010000000
VERSION     DATMJB010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2760
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797676"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VNU48_11120"
     CDS             <1..720
                     /locus_tag="VNU48_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWI11872.1"
                     /translation="TNSQNYFQKVPSPSPPTSENWLGTDQDSHDIVARLLYGFRVSIW
                     FAFALTLVGTVLGVLAGALQGYFGGRVDLFLQRIFEIWGAVPELYLLIIFASIFEPSL
                     LLLLLLLSLWGWLGLSDYVRAEFLRNRNLEYVKAARALGLSNGQIIWRHVLPNSLIPV
                     ITFLPFRMSGAIIALTSLDFLGLGVPQNTPSLGELLRQGKANLDAWWIMFPTFAVLVL
                     TMLLLTFIGDALRDAFDTRKS"
     gene            717..2324
                     /locus_tag="VNU48_11125"
     CDS             717..2324
                     /locus_tag="VNU48_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI11873.1"
                     /translation="MNDLSPASTTLLEIEHLRVRFDQSTVVDDVSFSIAPGEKFALVG
                     ESGSGKSITALSVLRLVDAAVTSGVIRFEGVDLLQRSERQMRGIRGAEIAMIFQEPMT
                     ALNPLYTVGNQIGEVLELHEGLRKNQARTRAIELLQRTGIPEPERRIDAFPHELSGGQ
                     RQRAMIAMALACRPKLLICDEPTTALDVTIQAQILALLDELQAEMGMALLFITHDLNL
                     VRRFTHRVGVMERGRLVELGATAAVFGAPQHAYTRKLLASRPQRVVQPLPADAAILLA
                     GDDIGVSFAIAQGWFSKRVFEAVRHATLRLKRGETLGIVGESGSGKTTLGMALLALQP
                     ISAGQVAMGGQRIDDADRNVLRAMRKRMQVVFQDPFASLSPRLTVGQIVGEGLALHRP
                     ELTKSARDRLVLDMLDEVGLSEAQGVANVLQRYPHEFSGGQRQRIAIARAVVLRPEVL
                     VLDEPTSALDVSVQQQVLALLAQLQQHYGMSYVFISHDLAVVRAMSHRVMVMKNGDVI
                     EEGEALALFDAPREDYTKALLAAAHLR"
     gene            2433..>2760
                     /locus_tag="VNU48_11130"
     CDS             2433..>2760
                     /locus_tag="VNU48_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWI11874.1"
                     /translation="MNITTTPTSFSVQRSRQVERLVRGQPTSDGDGVKLTRVLTQPLQ
                     KRLDPFLMLDAFGSDAASDYIGGFPSHPHRGFETVTIMLEGRMRHQDSVGNVSLLEPG
                     SVQWMTA"
BASE COUNT          413 a          941 c          952 g          454 t
ORIGIN      
        1 accaattcgc agaactactt ccagaaggtg ccgagtccgt cgccgccgac cagcgagaac
       61 tggctcggca ccgatcagga cagccacgac atcgtcgccc gcctgctcta cggctttcgc
      121 gtcagcatct ggttcgcgtt cgcgctgacg ctggtgggca ccgtgctggg cgttctcgcg
      181 ggcgcgctgc agggctattt cggcggtcgc gtcgacctgt tcctgcagcg catcttcgag
      241 atctggggcg cggtgcccga gctgtacctg ctgatcatct tcgcgtcgat cttcgagccc
      301 tcgctgctgc tgctgttgct gctgctgtcg ctgtggggct ggctcggcct gtcggactac
      361 gtgcgcgccg agttcctgcg caaccgcaac ctcgagtacg tgaaggcggc gcgcgcgctc
      421 ggcctgtcga acggccagat catctggcgc cacgtgctgc cgaactcgct gatcccggtg
      481 atcaccttcc tgccgtttcg catgagcggg gcgatcatcg cgctgacctc gctcgacttc
      541 ctcggtctgg gcgtgccgca gaacacgccg tcgctgggcg agctgctgcg ccagggcaag
      601 gccaacctcg atgcctggtg gatcatgttc ccgaccttcg cggtgctcgt gctgacgatg
      661 ctgctgctga ccttcatcgg cgacgcgctg cgcgatgcct tcgacacgcg caagtcatga
      721 acgacctcag tcccgcgtcg accaccttgc tcgagatcga gcacctgcga gtgcgcttcg
      781 accagagcac cgtcgtcgac gacgtgtcgt tctcgatcgc gccgggcgag aagttcgcgc
      841 tggtcggcga atcgggctcg ggcaagtcga tcaccgcgtt gtcggtgctg cggctggtcg
      901 acgccgcggt cacgagcggc gtgatccgct tcgagggcgt ggacctgctg cagcgctcgg
      961 agcgccagat gcgcggcatc cgcggcgcgg agatcgcgat gatcttccag gaaccgatga
     1021 ccgcgctgaa cccgctgtac acggtaggca accagatcgg cgaggtgctg gagctgcacg
     1081 agggcctgcg caagaaccag gcgcgcacac gtgcgatcga gctcttgcag cgcaccggca
     1141 tccccgagcc cgagcggcgc atcgacgcgt tcccgcacga gctgtcgggc ggccagcgcc
     1201 agcgcgcgat gatcgcgatg gcgctggcgt gccggcccaa gctgctgatc tgcgacgagc
     1261 cgacgaccgc gctggacgtg acgatccagg cacagatcct cgcgctgctc gacgagctgc
     1321 aggccgagat gggcatggcg ctgctgttca tcacccacga cctgaacctg gtgcggcgct
     1381 tcacgcaccg cgtcggcgtg atggagcgcg gccggctcgt cgaactcggc gccaccgccg
     1441 cggtgttcgg cgcgccgcag cacgcctaca cgcgcaagct gctggccagc cgcccgcagc
     1501 gcgtggtgca gccgctgccg gccgacgccg cgatcctgct ggccggcgac gacatcggcg
     1561 tgagtttcgc gatcgcgcaa ggctggttca gcaagcgcgt gttcgaggcg gtgcggcacg
     1621 cgacgctgcg cttgaagcgc ggcgagacgc tgggcatcgt cggcgagtcc ggctcgggca
     1681 aaaccacgct gggcatggcg ctgctcgcgc tgcagccgat ctcggccggc caggtcgcga
     1741 tgggcggcca gcgcatcgac gacgccgacc gcaacgtgct gcgcgcgatg cgcaagcgca
     1801 tgcaggtggt gttccaggat ccgttcgcat cgctgagccc gcgcctgacg gtcgggcaga
     1861 tcgtcggcga aggcctggcg ctgcatcggc ccgagttgac caagtcggcg cgcgacaggc
     1921 tcgtgctgga catgctcgac gaggtcggcc tgagcgaggc gcagggcgtg gccaacgtgc
     1981 tgcagcgcta cccgcacgag ttctcgggcg gccagcgcca gcgcatcgcg atcgcacgcg
     2041 cggtggtgct gcgccccgag gtgctggtgc tcgacgagcc gacctcggcg ctcgacgtct
     2101 cggtgcagca gcaggtgctg gcgctgctcg cgcagctgca gcagcactac ggcatgagct
     2161 atgtgttcat cagccacgat ctcgcggtgg tgcgtgcgat gtcgcaccgc gtgatggtga
     2221 tgaagaacgg cgacgtgatc gaggaaggcg aggcgctcgc gctgttcgac gcgccgcgcg
     2281 aggactacac caaggcgctg ctggcggcgg cgcacttgcg ctgagcggcc gagcccgtcg
     2341 aggcggccgc agcgcgtagg gttcaagcgc tttgaaggct gcgtgcagcc ttcggccctt
     2401 tcgccgggcc gcgatggaac tacattgaac gcatgaacat caccaccacc cccacctcct
     2461 tttcggtcca gcgctcgcgt caggtcgaac ggctcgtgcg tggccagccc acgtcggacg
     2521 gcgacggcgt caagctcacc cgtgtgctga cgcagccgct gcaaaagcgc ctcgatccgt
     2581 tcctgatgct cgacgccttc ggcagcgacg ccgcgagcga ctatatcggc ggcttcccga
     2641 gccacccgca ccgcggtttc gagaccgtga cgatcatgct ggaaggccgc atgcggcacc
     2701 aggacagcgt cggcaacgtc agcctgctcg agcccggcag cgtgcagtgg atgaccgccg
//
LOCUS       DATMJB010000516         2674 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_385351, whole genome shotgun sequence.
ACCESSION   DATMJB010000516 DATMJB010000000
VERSION     DATMJB010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385351"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VNU48_11135"
     CDS             <1..980
                     /locus_tag="VNU48_11135"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI11875.1"
                     /translation="VRQHAGPKWADGRIIKDPFPTAFGEGQQAFDLNLRRAKFQDIRV
                     REAVTLSYDFEMTVNRYGEYTRGDSMFSNTDFAAQGLPSPAELKLLEPFRNELPVTVF
                     GPAYVAPRTNGDPIKLRRNLLKARELFNAAGWKLAPDGRLRNAAGEAFEVEYMRPGEG
                     SNNLPEWQQNLEKLGGRLRFRDVDFALFQRRLEEYDFDMVAIVEGKFTLPPVVDLVGL
                     YGSKSADEKGNSNYRGVKSAAVDSLLKAMADATTIDELRTASRALDRVVMWNHWQVPG
                     YYTANERASYWDRFGKPAIRPKYYTVESPSDPQPAWPITTWWIKPGAAK"
     gene            1003..2040
                     /locus_tag="VNU48_11140"
     CDS             1003..2040
                     /locus_tag="VNU48_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWI11876.1"
                     /translation="MLNYLLKRILLMVPTLFGALAITFVVIQFVPGGPVEQLMAEARA
                     SQRGGGEGGYRASRDNDAKQIAALKKQYGFDKPAHVRFVEMIGNFLRFDLGRSFLQNK
                     DVWTLIKEKLPVSISLGLWTFLFTYLISIPLGVVKAVREGTRFDALTSLVVLIGYAIP
                     GFVLGLFLIVLFAGGTFWELFPLRGLTSDNWAELSWPARITDYFWHITLPLICMVIGS
                     FAIVTMLTKNTFVEEMRKQYVLVARAKGLSQKRVLFKHIFRNALIPLITGFPAAFIGA
                     FFAGSVLIETLFSLDGLGLLSYESVVRRDYPVVLGSLYLFTLIGLVVKLIADLLYVLV
                     DPRVQFESVGK"
     gene            2043..>2674
                     /locus_tag="VNU48_11145"
     CDS             2043..>2674
                     /locus_tag="VNU48_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI11877.1"
                     /translation="MSAIPAALPPVNEVSHVQVTTSVPMSPNQRAWARFKRNRLGYYS
                     LWVFGVLLVLATFAELISNDRPLVARYDGKIYFPLIANPPERQFGGDFGTPTDWKDPL
                     IRAQFAKPGNWALFTLNEHSDQSQDYFQKAPSPAAPSADDLLGTDGDSQDMVAKLIYG
                     FRVSIWFAFALTVVGTVLGVLAGAVQGYFGGRTDLFLQRIFEIWGAVPEL"
BASE COUNT          443 a          904 c          876 g          451 t
ORIGIN      
        1 gggtgcgcca gcacgcgggg ccgaagtggg ccgacggccg catcatcaag gatccgttcc
       61 cgaccgcctt cggcgaaggg cagcaggcct tcgacctgaa tctgaggcgc gcgaagttcc
      121 aggacatccg cgtgcgcgag gccgtcaccc tcagctacga cttcgagatg acggtcaatc
      181 gctacggcga gtacacgcgc ggcgacagca tgttcagcaa caccgacttc gcggcccagg
      241 gcctgccgtc gccggccgag ctgaaactgc tcgagccgtt tcgcaacgag ctgcctgtca
      301 ccgtgttcgg cccggcctac gtggcgccgc gcaccaacgg cgacccgatc aagctgcgtc
      361 ggaacctgct gaaggcgcgc gagctgttca atgccgccgg ctggaagctg gcccccgacg
      421 gccgcctgcg caatgccgcc ggcgaggcct tcgaggtcga gtacatgcgc cccggcgagg
      481 gcagcaacaa cctgccggaa tggcagcaga acctcgagaa gctcggcggc cggctgaggt
      541 tccgcgacgt ggacttcgcg ttgttccagc gccgcctgga ggagtacgac ttcgacatgg
      601 tcgccatcgt cgagggcaag ttcacgctgc cgcccgtggt cgacctggtc ggcctgtacg
      661 ggtcgaagtc ggccgacgag aagggcaaca gcaactatcg cggcgtcaag agcgcggccg
      721 tcgacagcct gctcaaggcc atggccgacg cgaccacgat cgacgagctg cgcaccgcca
      781 gccgcgcgct ggaccgggtc gtgatgtgga accactggca ggtgccgggc tactacaccg
      841 cgaacgagcg cgcctcgtac tgggaccgct tcggcaagcc ggcgatacgg ccgaagtact
      901 acacggtcga gagcccgagc gacccgcagc ccgcgtggcc gatcacgacc tggtggatca
      961 agcccggggc cgcgaagtga cggcggatcc gaaagcgcaa ccatgctgaa ctacctcctc
     1021 aaacgcatcc tgctgatggt gcccacgctg ttcggcgcgc tcgcgatcac cttcgtcgtg
     1081 atccagttcg tgcccggagg gccggtcgaa cagctgatgg ccgaagcgcg cgccagccag
     1141 cgcggcgggg gcgagggcgg ctatcgcgcg tcacgcgaca acgacgccaa gcagatcgcg
     1201 gcactgaaga agcagtacgg tttcgacaag cctgcgcacg tgcgcttcgt cgagatgatc
     1261 ggcaacttcc tgcgcttcga cctgggccgc agcttcctgc agaacaagga cgtgtggacg
     1321 ctgatcaagg agaagctgcc ggtctcgatc agcctgggcc tgtggacctt cctgttcacc
     1381 tacctgatct cgatcccgct cggcgtcgtg aaggcggtgc gcgaaggcac gcgcttcgat
     1441 gcgctgacct cgctggtggt gctgatcggc tacgcgatcc cgggcttcgt gctgggcctg
     1501 ttcctgatcg tgctcttcgc cggcggcacg ttctgggagc tgttcccgct gcgcggcctc
     1561 acgtcggaca actgggccga gctgagctgg ccggcgcgca tcaccgacta cttctggcac
     1621 atcacgctgc cgctgatctg catggtgatc ggcagcttcg cgatcgtgac gatgctgacc
     1681 aagaacacct tcgtcgaaga gatgcgcaag cagtacgtgc tggtggcgcg cgccaagggc
     1741 ctgtcgcaga agcgggtgct gttcaagcac atcttccgca acgcgctgat cccgctgatc
     1801 accggcttcc cggccgcctt catcggcgcg ttcttcgccg gctcggtgct gatcgagacg
     1861 ctgttctcgc tcgacggatt gggcctgctg tcgtacgaat cggtggtgcg gcgcgactac
     1921 ccggtggtgc tcgggtcgct ctatctgttc acgctgatcg gactggtcgt caagctgatc
     1981 gccgatctgc tgtacgtgct ggtcgacccc cgcgtgcagt tcgaaagcgt gggcaagtga
     2041 gcatgagcgc catccccgcc gccctgccgc cggtgaacga ggtctcccac gtgcaggtga
     2101 cgacatcggt gccgatgtca cccaaccagc gcgcctgggc gcgcttcaag cgcaaccggc
     2161 tgggctacta ctcgctctgg gtcttcggcg tgctgctggt actcgccacc ttcgccgaac
     2221 tgatcagcaa cgaccgcccg ctggtggcac gctacgacgg caagatctac tttcccctga
     2281 tcgccaaccc gcccgagcgc cagttcggcg gcgacttcgg cacgcccacg gactggaagg
     2341 acccgctgat ccgcgcgcag ttcgccaagc ccggcaactg ggcgctgttc acgctgaacg
     2401 agcactcgga ccagtcgcag gactacttcc agaaggcgcc cagccccgcc gcgccgagcg
     2461 ccgacgatct gcttggcacc gacggcgact cgcaagacat ggtggccaag ctgatctacg
     2521 gctttcgcgt cagcatctgg ttcgcgttcg cgctgaccgt cgtcggcacc gtgctgggcg
     2581 tgctggccgg cgcggtgcag ggctacttcg gcggccgcac cgatttgttc ctgcagcgca
     2641 tcttcgagat ctggggcgcg gtgcccgagc tgta
//
LOCUS       DATMJB010000517         5245 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_566021, whole genome shotgun sequence.
ACCESSION   DATMJB010000517 DATMJB010000000
VERSION     DATMJB010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566021"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VNU48_11150"
     CDS             <1..1449
                     /locus_tag="VNU48_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HWI11878.1"
                     /translation="LALANPEPEIRPELAQSVRPDCIIATGRSDYPNQVNNVLCFPYI
                     FRGALDSGATSITEAMKVACVREIADLAKAEISAEVASAYAGRELRFGPDYLIPTPFD
                     SRLILRIAPAVAKAAAASGVATRPIADMDAYRQSLLRFVTHTGMFMRPVFGAARADPK
                     RVAYAEGEDERVLRAVQVALDEKLVKPILIGRPAVIAMRIERAGLRLQAGRDFEVVNP
                     EDDARFRQYWETYHALNARDGVTPEMAKAAVRRSNTTIAALMVHLGDADGMLCGLVGR
                     YQNHLGHIDEVIGKRKSAHVYAALNALMLPKHTLFITDAYVNEDPSAEQLADIAAMAV
                     EAVRHFGLPPRVAFLSHSAYGSSKRPSALKMRKARDLFVARMPDVPADGEMHGDLALD
                     EAMRTSLMPDSTLSGSANLLVLPNLDAANILYNVLKMTGGQGVTVGPILLGVNAPAHI
                     LTPAATVRRVVNMTALAVAHAGALAARRAGGE"
     gene            complement(1471..2709)
                     /locus_tag="VNU48_11155"
     CDS             complement(1471..2709)
                     /locus_tag="VNU48_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI11879.1"
                     /translation="MNSDDRQDDFDRGVKNRRAVLGDAWVDKSLNNATEFNAEFQALI
                     TRYAWHDIWGRPGLDHTTRRLLVLGMTMGLARWEEFELHCRATIRGGVPLGLIKETLM
                     QGAIYCGVPAANTAFKLTMDILRDEGLLPAPAPLAGATRVAVHHTFSQPQLRVALQGP
                     PGPGGGAAPIVLSHALGLDLHLWDELAAQLAAQQHPVLRYDHRGHGGSAVPAGPYTMD
                     ELVDDAARLIREWGRGPVVWVGLSMGGMVGQGLAVRYPELVQALVLANTIAKYPEAAQ
                     AMWAQRIATVEAQGLAGIVEMVLQRYFTDAFRAAEPQAVAGFRSRVLRTDAAGYVACC
                     HAVANVDWLERLRSVRCPTLVIAGALDVGAPVAMSQAMAERIPQAELVVLDNASHLSA
                     VEQPAAFAATLGAFLRRLPA"
     gene            complement(2750..4060)
                     /locus_tag="VNU48_11160"
     CDS             complement(2750..4060)
                     /locus_tag="VNU48_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWI11880.1"
                     /translation="MDVALLFCLILLNALFAMSEMALTASRKARLQVMLEAGESGAQA
                     ALDLHENPTKFLSVVQVGITSISILNGIVGDAAFSAPFARWLHETFPIAQRASEITAT
                     AMVVVTVTFITIIFGELVPKRFGLMYPEAVARVVSRPMEWLSLITRPFIKLLSWCTET
                     TLRLFGITGNPDRSVTEEEINASLEEGLDAGIIEAQEHQMVRNVFRLDDRQVGSMMIP
                     RAEIVWLDVAASSDEVLVLIGDEEHSRYPVCRGGLDDVLGVISAQTLLQQVMKGHAMS
                     LADGLQPSVFVPETLSGMELLEHFRASSAQLVFVVDEYGEVQGMITVRDVLEAITGEF
                     ATPTADDAWAVQRADGSWLFDGLIPVPELKDRLDMKELPEEDRGRYNTLAGMIMLLLG
                     RLPRTTDSVEWDHWRFEVVDLDGKRVDKVLASRLPFEPNAGETD"
     gene            4132..4935
                     /locus_tag="VNU48_11165"
     CDS             4132..4935
                     /locus_tag="VNU48_11165"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HWI11881.1"
                     /translation="MNYLIGDVQGCCGALDALLAKIDFSASRDRLYVLGDLVNRGPQS
                     LQTLRRLRSFGDSAVCLLGNHDLNLLAIAQGARKPHAGDTIGEILDAPDREVWLDWLR
                     QRRMAVFEHGWLMVHAGVVPQWDAAQTLALAAEVEALLRGDALAAFLPQMYGDQPTRW
                     SDTLQGAERWRFVINVLTRVRFVAADGTLDLVTKEGAGGALPGFFPWFEAPQRRSAGT
                     PIAFGHWASLGLVNRTDLLALDTGCVWGRQLSAARIDGGRREIIQVDCG"
     gene            5028..5117
                     /locus_tag="VNU48_11170"
     tRNA            5028..5117
                     /locus_tag="VNU48_11170"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5062..5064,aa:Ser,seq:tga)
BASE COUNT          816 a         1870 c         1789 g          770 t
ORIGIN      
        1 ctcgcgctcg ccaatccgga gccggagatc cgccccgagc tcgcgcagag cgtgcgcccc
       61 gactgcatca tcgcgaccgg ccgctcggac tacccgaacc aggtcaacaa cgttctctgc
      121 ttcccgtaca tcttccgcgg cgcgctcgac agcggcgcga ccagcatcac cgaagcgatg
      181 aaggtcgcct gcgtgcgcga gatcgccgat ctcgccaagg ccgagatcag cgccgaggtc
      241 gcttcggcgt atgcgggccg cgagctgcgc ttcggccccg actacctgat cccgacgccg
      301 ttcgactcgc gcctgatcct gcgcatcgcg ccggcggtcg cgaaggccgc cgcggcctcg
      361 ggcgtcgcga cccggccgat cgccgacatg gacgcctacc gccagtcgct gctgcgcttc
      421 gtcacccaca ccggcatgtt catgcgcccg gtgttcggcg cggcccgcgc cgacccgaag
      481 cgcgtggcgt acgccgaggg cgaggacgag cgcgtgctgc gcgcggtgca ggtcgcgctc
      541 gacgagaagc tcgtcaagcc gatcctgatc ggccgccccg cggtgatcgc gatgcgcatc
      601 gaacgcgccg ggctgcgctt gcaggccggg cgcgatttcg aggtcgtgaa cccggaagac
      661 gacgcacgct tccgccagta ctgggagacc taccacgcgc tcaacgcccg tgacggcgtc
      721 acgccggaga tggccaaggc tgcggtgcgg cgctcgaaca cgacgatcgc cgcgctgatg
      781 gtgcacctcg gcgacgccga cggcatgctg tgcgggctgg tcggccgcta ccagaaccac
      841 ctcggccaca tcgacgaggt catcggcaag cgcaagagtg cccacgtcta cgccgcgctg
      901 aacgcgctga tgctgcccaa gcacacgctg ttcatcaccg acgcctatgt caacgaagac
      961 ccgagcgccg agcagctcgc cgacatcgcg gcgatggcgg tcgaggcggt gcgccacttc
     1021 ggcctgccgc cgcgcgtggc gttcctgtcg cactcggcct atggcagcag caagcggccg
     1081 tcggcgctga aaatgcgcaa ggcccgcgac ctgttcgtcg cgcgcatgcc cgacgtgccg
     1141 gccgacggcg agatgcacgg cgacctcgcg ctcgacgagg cgatgcgcac atcgctgatg
     1201 cccgactcga cgctctccgg cagcgcgaac ctgctcgtgc tgccgaatct cgatgccgcg
     1261 aacatcctct acaacgtgct gaagatgacc ggcggccagg gcgtgacggt cggcccgatc
     1321 ctgctcggcg tgaacgcacc ggcgcacatc ctgacgcccg cggcgacggt gcggcgcgtc
     1381 gtcaacatga ccgcgctggc ggtggcacac gcgggcgcgc tggcagcccg ccgcgcgggc
     1441 ggcgagtaag cgcggcgacg ggctcgctcg tcaggccggc agccggcgca ggaaggcgcc
     1501 gagcgtcgcg gcaaacgccg caggctgctc gaccgcgctc aggtgcgagg cgttgtcgag
     1561 caccaccagc tcggcctgcg ggatgcgctc agccatcgcc tgcgacatcg ccaccggcgc
     1621 gccgacatcg agggcaccgg cgatcaccag cgtcgggcag cgcacgctgc gcagacgctc
     1681 gagccagtcg acgttggcca cggcatggca gcaggccacg tagccggccg cgtcggtgcg
     1741 cagcacgcgg ctgcggaagc ccgccacggc ctgcggctcg gcggcacgaa acgcgtcggt
     1801 gaagtagcgc tgcaggacca tctcgacgat gccggccagg ccctgcgctt cgaccgtcgc
     1861 gatgcgctgc gcccacatcg cctgcgccgc ttccgggtac ttcgcgatgg tgttcgcgag
     1921 caccagggcc tggacgagct ccgggtatcg cacggccagg ccctgcccaa ccatgccacc
     1981 catcgacagc ccgacccaca cgaccgggcc gcggccccat tcgcggatca gccgggcggc
     2041 atcgtcgacc agttcgtcca tcgtgtaggg cccggccggc accgccgagc cgccgtggcc
     2101 gcggtggtcg tagcgcagca ccgggtgctg ctgcgcggcg agttgcgcgg cgagctcgtc
     2161 ccacaggtgc aggtccaggc cgagcgcgtg gctgagcacg atcggcgcgg cgccgccacc
     2221 cggcccgggc ggcccctgca gcgcgacgcg cagctgcggc tggctgaagg tgtggtgcac
     2281 ggcgacgcgc gtcgcgccgg cgagcggcgc cggcgcgggc agcaggccct cgtcgcgcag
     2341 gatgtccatc gtcagcttga acgcggtgtt cgcggccggc acgccgcagt agatcgcgcc
     2401 ctgcatcagg gtttccttga tgagccccag cggcacgcca ccgcggatcg tggcgcggca
     2461 gtgcagctcg aactcctccc agcgcgccag ccccatcgtc atgcccagca cgagcaggcg
     2521 gcgcgtcgtg tggtccagcc ccggccggcc ccagatgtcg tgccacgcat agcgcgtgat
     2581 caaggcctgg aactcggcgt tgaactcggt cgcattgttc agcgacttgt cgacccacgc
     2641 atcgccgagc acggcgcggc ggttcttcac gccgcggtcg aagtcgtcct gtcggtcgtc
     2701 gctgttcatg gcgtcacctc tttcgactgg aatcgccgcg cgactcgatt caatcggttt
     2761 ctccggcatt gggttcgaag ggcaggcggc tcgcgaggac cttgtcgacg cgcttgccgt
     2821 cgaggtcgac gacctcgaag cgccaatggt cccactccac cgaatcggtg gtgcgcggca
     2881 ggcgcccgag cagcagcatg atcatgccgg ccagcgtgtt gtagcggccg cggtcttctt
     2941 cgggaagctc cttcatgtcg aggcggtcct tcagctcggg caccgggatc aggccgtcga
     3001 acagccagct gccgtcggcg cgctgcaccg cccaggcgtc gtcggcggtc ggcgtggcga
     3061 actcgccggt gatcgcctcc agcacgtcgc gcacggtgat catgccctgc acctcgccgt
     3121 attcgtcgac gacgaacacg agctgcgcgc tcgaggcacg aaagtgttcg agcagctcca
     3181 tgcccgacag ggtctcgggc acgaacaccg acggctgcag cccgtcggcc agcgacatcg
     3241 cgtggccctt catgacctgc tgcagcagcg tctgcgccga gatcacgccg agcacgtcgt
     3301 ccaggccgcc gcggcacacc gggtagcgcg aatgttcctc gtcgccgatc agcaccagca
     3361 cctcgtcgct cgacgcggcc acgtcgagcc agacgatctc ggcgcgcggg atcatcatcg
     3421 agccgacctg gcggtcgtcg aggcggaaca cgttgcgcac catctggtgc tcctgcgcct
     3481 cgatgatgcc ggcgtccagg ccctcctcga ggctggcgtt gatctcttcc tcggtgacgc
     3541 tgcggtccgg gttgccggtg atgccgaaca ggcgcagcgt ggtctcggtg caccacgaca
     3601 gcagcttgat gaacggccgc gtgatcagcg acagccactc catcggccgc gacaccacgc
     3661 gcgccaccgc ctcggggtac atcagcccga agcgcttggg cacgagctcg ccgaagatga
     3721 tcgtgatgaa ggtgacggtg acgacgacca tcgcggtcgc ggtgatctcg gacgcgcgct
     3781 gcgcgatcgg gaaggtctcg tgcagccagc gcgcgaacgg cgccgagaac gcagcgtcgc
     3841 cgacgatgcc gttcaggatg ctgatcgacg tgatgcccac ctgcaccacc gagaggaact
     3901 tggtcgggtt ttcgtgcagg tcgagcgccg cctgtgcccc cgattcgccc gcttccagca
     3961 tcacctgcag gcgggccttg cggctcgccg tgagcgccat ttcggacatg gcgaacagcg
     4021 cgttcagcag gatcaggcag aacagaaggg caacgtccat gcggcgcgcg cggcaatcga
     4081 ggaacggcca gcgtagcaga aacgccaatc gcgcccggct agcatccggg catgaactac
     4141 ctgatcggcg acgtgcaagg ctgctgcggt gcgctcgatg cgctgctcgc gaagatcgac
     4201 ttctcggcgt cgcgcgaccg cctctatgtg ctcggcgatc tggtcaaccg cggcccgcag
     4261 tcgttgcaga cactgcgccg gctgcgcagc ttcggcgact cggcggtctg cctgctcggc
     4321 aaccacgacc tgaacctgct cgcgatcgcg cagggcgcac gcaagccgca cgcgggcgac
     4381 acgatcggcg agattctcga cgcacccgac cgagaggtct ggctcgactg gctgcgccag
     4441 cgccgcatgg cggtgttcga gcacggctgg ctgatggtgc acgccggcgt cgtgccgcaa
     4501 tgggacgcgg cgcagacgct ggccctggcc gccgaggtcg aggcgctgct gcgcggcgac
     4561 gcgctcgccg cgttcctgcc gcagatgtac ggcgaccagc cgacgcgctg gagcgacacg
     4621 ctgcaaggcg ccgaacgctg gcgcttcgtc atcaacgtgc tgacgcgcgt gcgtttcgtc
     4681 gcggccgacg gcacgctcga cctcgtcacg aaggaaggcg ccggcggcgc cctgcccggc
     4741 ttcttcccat ggttcgaggc gccgcagcgg cgcagcgccg gcacgccgat cgcgttcggc
     4801 cactgggcca gcctcgggct ggtcaatcgc acggatctgc tcgcgctcga caccggctgc
     4861 gtctggggcc ggcaactctc ggcggcgcgt atcgacggcg ggcggcgcga gatcatccag
     4921 gtcgactgcg gctgagacgc tgagcccgga acgcagcgcg actcgcgcct cgcgccgtgt
     4981 tcgacgcgct cgagcgatca ttacaatggc agcggtggtc tttcgacgga gacgtggccg
     5041 agcggtttaa ggctgcagtc ttgaaaactg ccgagggggc aactcctccg tgagttcgaa
     5101 tctcaccgtc tccgccagat tgccaggaaa tatgcgggtt tcctggctgt aaggctgacc
     5161 tgcccctcaa tcagcccgcc ttgaaaaaga aggcctcaat ggcgcgcgcc gaagagcaac
     5221 tgggcgatga ctgagcacaa gttcg
//
LOCUS       DATMJB010000518         3993 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_434855, whole genome shotgun sequence.
ACCESSION   DATMJB010000518 DATMJB010000000
VERSION     DATMJB010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434855"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VNU48_11175"
     CDS             complement(<1..1129)
                     /locus_tag="VNU48_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWI11882.1"
                     /translation="MKFASPSLTRHVFFSLTIGAALVVAAGTAMAQAKPLAGQTVKIA
                     MIEGLSGPFANVGQSQLKNWQLVAEHFANKNPAGVRFEIVGMDSKSSPQEALNLLRAA
                     SDQGFRYVTQGNGSNVAVPLADAVAKHNERNPGKEIVYLNYAAVDPVLTNEKCSFAHF
                     RFDADTSMKMAALTSFMKGQPKISKVYLINQNYSHGQQVSKYFKEGMTRNRPDAKIVG
                     DDLHPLGQVKDFSPYVAKIKQSGADAIVTGNWGQDMTLLIKALNDSGLKLPIYAYYAG
                     VSGTPTALASGGDLEVYQIAYNHSNYGGEVGQLMADFKKKYNDDFYTFSIYNAIVLLG
                     EAMAKAKSADPMKVAFAMEGLKFKGFNGESEIRKTDHQMQQG"
     gene            1261..2817
                     /locus_tag="VNU48_11180"
     CDS             1261..2817
                     /locus_tag="VNU48_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HWI11883.1"
                     /translation="MSAAIAKLAAMNMAELAANNPPGPEAPLHDWPNRFRGLGEAFFT
                     ALPAEPLPDPHWVATSDDCAALLGLPPDWATRPELNALEVFSGNARWPGMHTLASVYS
                     GHQFGVWAGQLGDGRALWLGEIATPRGPMELQLKGAGRTPYSRMGDGRAVLRSSIREF
                     LGSEAMHALGIPTTRALCVTGSALPVRRETVETAAVTTRVAPSFIRFGHFEHFAHHGL
                     HGELRRLADFVIEHHDPACRDAANPYAALLEAVARRTAVLMADWQAVGFCHGVMNTDN
                     MSVLGLTIDYGPFGFLDQFDPGHICNHSDHQGRYAWARQPNVAFWNLHALAQALLPLI
                     EDSDRALAALEPYKTSFADTLLARWRAKLGLRTSEADDRELVDDLLRLMAAERCDFTI
                     TFRRLAGFSTAAGADNAAIRDLFIDRAAFDVWAARYAARLAHEAGVDAADDGARALRM
                     NRVNPKFVLRNHLAEGAIRAAQGGDFGETRRLLKVLQRPFDEQDASASAAYAGFPPEW
                     AHSIEVSCSS"
     gene            2814..3230
                     /gene="msrB"
                     /locus_tag="VNU48_11185"
     CDS             2814..3230
                     /gene="msrB"
                     /locus_tag="VNU48_11185"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017773882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HWI11884.1"
                     /translation="MKDDASDTKYPVKKTEAEWRAQLDPIQFEVARHAATERPFSGKY
                     WDHWSEGQYQCVGCGTPLFRSDTKFDAGCGWPSYYEPVNGEVVERIVDKSHGMVRVEV
                     RCNKCGSHLGHVFPDGPEPTGERFCINSAAIDFAPK"
     gene            3256..3906
                     /locus_tag="VNU48_11190"
     CDS             3256..3906
                     /locus_tag="VNU48_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HWI11885.1"
                     /translation="MKIFLDFLPLLLFFVVGKYADSHAEWAARFATDHFGFMVSGGVV
                     GTEEASALLATIVVIVATAVQIGWLMSRRKKVDVMLWVTFALVVVLGGATIWFHNPTF
                     IKWKPSALYWAMGLAFWVSQAIFHKNLLQTLIGAQLQLPAAIWQRLNFVWIAFFGLLG
                     LLNLYVAYTFSTSTWFTFKAFGVTGLMLLFMLAQGLYIGKHLEPEPGPAPAPDRQP"
BASE COUNT          629 a         1338 c         1300 g          726 t
ORIGIN      
        1 gcccttgctg catctgatga tcggtcttgc ggatctcgct ctcgccgttg aagcccttga
       61 acttcagccc ctccatcgcg aacgcgacct tcatcggatc ggccgacttc gccttggcca
      121 tcgcctcgcc gagcagcacg atcgcgttgt agatcgagaa ggtgtagaag tcgtcgttgt
      181 atttcttctt gaagtccgcc atcagctggc cgacttcgcc gccgtagttg gagtggttgt
      241 acgcgatctg atagacctcg agatcgccac ccgaggccag cgccgtgggc gtgcccgaga
      301 cgcccgcgta gtacgcgtag atcggcagct tcaggcccga gtcgttcagc gccttgatca
      361 gcagggtcat gtcctgaccc caattgccgg tgacgatcgc gtcggccccc gactgcttga
      421 tcttggcgac gtagggcgag aagtccttca cctggcccag ggggtgcaga tcgtcgccga
      481 cgatcttggc gtccgggcgg ttgcgcgtca tgccttcctt gaagtacttc gacacctgct
      541 ggccgtgcga gtagttctgg ttgatcaggt agaccttcga gatcttcggc tggcccttca
      601 tgaacgaggt cagcgcggcc atcttcatcg acgtgtcggc atcgaagcgg aagtgcgcga
      661 agctgcactt ctcgttcgtc agcaccgggt cgaccgccgc atagttcagg tacacgatct
      721 ccttgccggg gttgcgctca ttgtgcttgg ccaccgcatc ggcgagcggc acggccacgt
      781 tcgaaccgtt gccctgcgtg acatagcgaa agccctgatc gctcgcggcc ctcagcaggt
      841 tcagtgcttc ctgcggcgag ctcttgctgt ccatcccgac gatctcgaac ctgaccccgg
      901 ccgggttctt gttggcgaaa tgttcggcga ccagctgcca gttcttgagc tggctctggc
      961 cgacgttggc gaacggaccc gacagtccct cgatcattgc gatcttcacg gtctggccag
     1021 ccagcggctt ggcctgcgcc atcgcggtgc cagcggcaac gaccagggcc gcgccgatgg
     1081 tgaggctgaa aaaaacgtga cgcgtcagcg acgggctagc aaacttcatg gaggtctccc
     1141 agggaaatgt gaacactcgc aaagatagcg gatgtgcctg gcgtggggtg acagagacta
     1201 ccccaagcac acggtgcacc ggggcggtga ttctcggagc taacgcccct gcgcgaatga
     1261 atgtcggccg cgatcgctaa actcgcggcg atgaacatgg ccgagcttgc cgcgaacaac
     1321 ccgccggggc ccgaagcccc gctgcacgac tggccgaatc gcttccgggg cctgggtgaa
     1381 gcgttcttca cggccctgcc cgccgagccc ttgcccgatc cgcattgggt cgcgaccagc
     1441 gacgactgtg ccgcgctgct cggtctgccg cccgactggg cgacacgacc cgaactgaac
     1501 gcgctcgagg tcttcagcgg caacgctcgt tggcccggca tgcacacgct ggccagcgtc
     1561 tacagcggcc accagttcgg cgtctgggcc ggacagttgg gcgacggccg cgcgctctgg
     1621 ctgggcgaaa tcgcgacgcc gcgcgggccg atggagctgc agctcaaggg cgccggccgc
     1681 acgccgtatt cgcgcatggg cgacggccgc gcggtgctgc gctcgtcgat ccgcgagttc
     1741 ctggggtcgg aggcgatgca tgcactgggc atcccgacga cgcgcgcgct gtgcgtcacc
     1801 ggttcggcgc tgccggtgcg ccgcgagacc gtcgagaccg ccgcggtgac cacgcgcgtc
     1861 gcgccgagct tcatccgctt cggccacttc gagcacttcg cccaccatgg gctgcacggc
     1921 gagctgcgcc ggctcgccga cttcgtgatc gagcatcacg accccgcctg tcgcgacgcg
     1981 gcgaacccgt acgcggcact gctcgaagcg gtcgcgcggc gcactgcggt gctgatggcc
     2041 gactggcagg cggtcggctt ctgtcatggc gtgatgaaca ccgacaacat gtcggtgctg
     2101 ggcttgacga tcgactacgg gccgttcggc tttctcgatc agttcgaccc cggccacatc
     2161 tgcaaccact ccgaccatca gggccgctat gcctgggcac gacagccgaa tgtcgcgttc
     2221 tggaacctgc atgcgctggc gcaggcgctg ctgccgctga tcgaggattc ggatcgggcg
     2281 ctcgctgcac tcgaacccta caagaccagc ttcgccgaca cgctgctggc gcgctggcgc
     2341 gccaagctcg gcctgcgcac cagcgaggcc gatgatcgcg agctggtcga cgatctgctg
     2401 cgcctgatgg ccgccgagcg ctgcgacttc acgatcacgt tccgccggct cgccggtttc
     2461 tcgaccgccg ccggcgccga caacgcggcc atccgcgatc tgttcatcga ccgcgcggcg
     2521 ttcgatgtct gggccgcgcg ctatgccgcg cggctggcgc acgaggccgg cgtcgatgcg
     2581 gccgacgacg gcgcgcgggc cttgcgaatg aaccgtgtga accccaagtt cgtgcttcgc
     2641 aaccaccttg ccgagggcgc gatccgcgcc gcgcagggcg gcgacttcgg cgagacccgg
     2701 cgcctgctga aagttctgca gcggcccttc gacgaacagg atgcgagcgc cagtgccgcg
     2761 tacgccggct tcccgcccga atgggcgcac tccatcgagg tttcctgctc atcatgaaag
     2821 acgacgcttc cgacaccaag tacccggtca agaagaccga agccgaatgg cgcgcccagc
     2881 tcgacccgat ccagttcgag gtcgcgcgcc acgccgccac cgagcggccg ttcagcggca
     2941 agtactggga tcactggagc gaagggcagt accagtgcgt cggctgcggc acgccgctgt
     3001 tccgctccga caccaagttc gacgccggct gcggctggcc gagctactac gagccggtga
     3061 acggcgaggt cgtcgagcgc atcgtcgaca agagccacgg catggtgcgg gtcgaggtgc
     3121 gatgcaacaa gtgcgggtcg catctgggtc acgtcttccc cgatgggccc gagccgaccg
     3181 gcgagcgctt ctgcatcaac tcggccgcga tagacttcgc gcccaaatga tgcccggcca
     3241 cgcgttggcg ccccgatgaa gatctttctc gactttctgc cgctgctgct gttcttcgtc
     3301 gtcggcaagt acgccgacag ccatgccgag tgggccgcgc gcttcgccac cgatcacttc
     3361 ggcttcatgg tctccggcgg cgtggtcggc accgaggagg cgtcggcgct gctcgcgacg
     3421 atcgtcgtga tcgtcgccac cgcagtgcag atcggctggc tgatgtcgcg ccggaagaag
     3481 gtggacgtga tgctgtgggt gaccttcgcg ctggtggtgg tgctgggcgg tgcgacgatc
     3541 tggttccaca acccgacctt catcaagtgg aagccgagcg cgctgtactg ggccatgggg
     3601 ctggcgttct gggtcagcca ggcgatcttc cacaagaacc tgctgcagac cctgatcggc
     3661 gcacagctgc agttgccggc cgcgatctgg cagcgcctga acttcgtctg gatcgcgttc
     3721 ttcggactgc tcgggctgct gaacctttac gtcgcctaca ccttcagcac ctcgacctgg
     3781 ttcaccttca aggccttcgg cgtgaccggc ctgatgctgc tgttcatgct ggcgcaaggc
     3841 ctgtacatcg gcaagcacct cgagcccgaa ccgggcccgg cgcccgcacc cgatcggcag
     3901 ccctgaaatg aaactggcag tctggagctg tggcggtgtg gcggcttgcg gccctttgcg
     3961 gtcgtttgca agcgagcgtg cgccctttga cgg
//
LOCUS       DATMJB010000519         2239 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_97624, whole genome shotgun sequence.
ACCESSION   DATMJB010000519 DATMJB010000000
VERSION     DATMJB010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97624"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VNU48_11195"
     CDS             complement(<1..480)
                     /locus_tag="VNU48_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HWI11886.1"
                     /translation="MPTIPAVAGVPVDFILFALTLAGVALFHHHTLRVAVTGLLAITA
                     YKLAFGGFRGVPGVDGLLALLGHEWVTVANLFGLLLGFALLSKHFEESQLPALLPRYL
                     PDDWKGGFAMLGLVFVLSSFLDNIAAAMIGGTMATVLFKGRLHVGFLAAIVAASNAGG
                     "
     gene            complement(492..1190)
                     /locus_tag="VNU48_11200"
     CDS             complement(492..1190)
                     /locus_tag="VNU48_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HWI11887.1"
                     /translation="MTPTSEQTEAVDTAIRTRRSLRAFLPTPVPRETIAAILAVAARA
                     PSGTNTQPWKVYVLTGKAKTELSRKIAAVYDDPAERAKHSDEYDYYPTEWKSPFIDRR
                     RKVGWDLYGLLDIAKTDKARMHAQHRRNYEFFDAPVGLIFTIDRVLRQGSWLDYGMFL
                     QSIMVAARGRGLDTCPQAAFMQFHRIITEHVGASADEQVVCGMSLGHADPDAIENTLV
                     TEREPVEAFTRFLD"
     gene            complement(1187..1651)
                     /locus_tag="VNU48_11205"
     CDS             complement(1187..1651)
                     /locus_tag="VNU48_11205"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HWI11888.1"
                     /translation="MTAPTPAPPTPAVRPTPPGRDAYRHFSAITTRWMDNDAYGHVNN
                     VVYYSWFDTVVNRYLIEAGALDIERSPAIGLVVETHCNYFAPLAFPQPVEAGLRVAHL
                     GGSSVRYEIGLFGPGQARAAACGHFVHVYVDRSTRRPAPLPAPLIQALRGLM"
     gene            complement(1648..>2239)
                     /locus_tag="VNU48_11210"
     CDS             complement(1648..>2239)
                     /locus_tag="VNU48_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HWI11889.1"
                     /translation="HASADKGGGFCVFNDAAVAARLMQAEWHRTRHQLLRVAIIDLDV
                     HQGNGSATIFADDPTVFTLSLHGAKNFPFRKAASDLDVALPDGCGETAYLRALDDALA
                     ELWRRHALAPPGLIFYLAGADPHEGDRLGRLKLTTAGLAERDRRVLAQARARRVPVAL
                     SMAGGYGRVVEDTVEVHVGTIRAALESWRQWNNARA"
BASE COUNT          346 a          749 c          816 g          328 t
ORIGIN      
        1 cccgccggcg ttcgatgcgg cgacgatcgc ggccaggaag ccgacgtgca accggccctt
       61 gaacagcacc gtcgccatcg tgccgccgat catcgccgca gcgatgttgt cgagaaagct
      121 cgacagcacg aacaccagcc ccagcatcgc gaagccgccc ttccagtcgt cgggcaggta
      181 gcgcggcagc agcgccggca actggctttc ctcgaagtgc ttggacagca gcgcgaaacc
      241 gagcagcagc ccgaacaggt tcgcgaccgt gacccactcg tgaccgagca gcgcgagcag
      301 cccgtcgacg cccggcacac cgcgaaagcc gccgaacgcg agcttgtacg cggtgatcgc
      361 gagcagcccg gtcaccgcga cgcgcagcgt gtggtgatgg aacagcgcga cgccggcgag
      421 cgtcagcgcg aacaggatga agtcgaccgg cacgccggcc accgcaggga tcgtgggcat
      481 gatggcgcgc gtcagtcgag gaagcgcgtg aacgcctcga ccggctcacg ctcggtcacc
      541 agcgtgttct cgatcgcatc cgggtcggcg tggccgagcg acatgccgca cacgacctgc
      601 tcgtcggccg aggcaccgac atgttcggtg atgatgcggt gaaactgcat gaaggcggcc
      661 tgcgggcagg tgtcgagacc gcgaccgcgc gccgcgacca tgatgctctg caggaacatg
      721 ccgtagtcga gccagctgcc ctgccgcagg acgcggtcga tcgtgaagat caggcccacc
      781 ggcgcgtcga agaactcgta gttgcggcgg tgctgcgcgt gcatgcgcgc cttgtcggtc
      841 ttggcgatgt cgagcaggcc gtacaggtcc cagccgacct tgcggcgccg gtcgatgaag
      901 ggcgacttcc attcggtcgg gtagtagtcg tactcatcgc tgtgcttcgc acgctcggcg
      961 ggatcgtcgt agaccgcggc gatcttgcgc gacagctcgg tcttggcttt gccggtcagc
     1021 acgtagacct tccacggctg cgtgttggtg cccgacggcg cgcgcgccgc cacggccagg
     1081 atcgccgcga tcgtctcgcg cggcaccggt gtgggcagga acgcgcgcag cgagcggcgt
     1141 gtgcggatcg cggtgtcgac ggcttcggtc tgttcggagg tcggggtcac atcagtcctc
     1201 gcaaggcttg aatcaggggg gccggcaacg gcgcgggccg gcgcgtcgag cggtcgacgt
     1261 agacatggac gaaatgtcca caggccgcgg cccgcgcctg gccgggaccg aacaggccga
     1321 tctcgtagcg cacgctcgag ccgcccaggt gcgcgacgcg cagcccggcc tcgacgggct
     1381 gcgggaacgc cagcggcgcg aagtagttgc agtgcgtctc gaccaccagc ccgatcgccg
     1441 ggctgcgctc gatgtcgagc gcgccggcct cgatcaggta gcggttcacg accgtgtcga
     1501 accagctgta gtagacgacg ttgttgacgt ggccgtaggc gtcgttgtcc atccagcgcg
     1561 tcgtgatggc gctgaaatgc cggtacgcgt cacgccccgg cggcgtgggg cggacggcgg
     1621 gcgtcggggg tgcgggtgtc ggtgcggtca tgcacgcgca ttgttccatt gccgccagct
     1681 ctcgagcgcg gcgcggatcg tgccgacatg gacctcgacc gtgtcctcga cgacgcgccc
     1741 gtagcccccg gccatcgaca gtgccaccgg gacgcggcgc gcacgggcct gcgcgagcac
     1801 gcggcggtcg cgctcggcga gcccggcggt cgtcagcttc aggcggccga gccggtcgcc
     1861 ctcgtgcggg tcggcaccgg cgaggtagaa gatcagcccc ggtggcgcga gcgcatggcg
     1921 ccgccacagc tcggcgagcg cgtcgtcgag cgcgcgcaga taggccgttt cgccacaacc
     1981 gtcgggcagg gctacgtcga ggtcgctcgc ggccttgcgg aacgggaagt tcttcgcgcc
     2041 gtgcagcgac agcgtgaaca cggtcgggtc gtcggcgaag atggtcgcgc tgccgttgcc
     2101 ctggtgcacg tcgaggtcga tgatcgccac gcgcagcagc tggtgccgcg tgcggtgcca
     2161 ttcggcctgc atcaggcgcg cggcgaccgc ggcgtcgttg aacacgcaaa agccgccgcc
     2221 cttgtcggcg ctcgcgtgg
//
LOCUS       DATMJB010000520         4229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_710881, whole genome shotgun sequence.
ACCESSION   DATMJB010000520 DATMJB010000000
VERSION     DATMJB010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710881"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VNU48_11215"
     CDS             complement(<1..1106)
                     /locus_tag="VNU48_11215"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWI11890.1"
                     /translation="MTSAVIVSTARTPLAKSWKGAFNMTHGATLGGLAVKAAVERAGI
                     DGAEVDDVIMGCGMPEGATGTNIGRQVALRAGLPVTTSAMTVNRFCSSGLQTIALAAQ
                     RIIAGESDVQVAGGVESISCVWNESNKHMLGDPWLAEHKPEIYWPMLQTAEQVAKRYK
                     IGRERMDRYGAASQQRATAALEAGKFSDEIIECTVMAGFADPVQGLRTKEVTIKADEG
                     IRPGTTYESIMGIKTAIPGGVVAAGNASQLSDGGGACVLMSETLASKRNLKPLGRFLG
                     FAVAGCEPDEMGIGPVFAVPKVLKKLGLTVADIDLWELNEAFAVQVLYCADTLGIPMD
                     KLNVNGGAIAVGHPYGMSGQRLTGHALIEGKRRGA"
     gene            complement(1123..1725)
                     /locus_tag="VNU48_11220"
     CDS             complement(1123..1725)
                     /locus_tag="VNU48_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWI11891.1"
                     /translation="MNDRATFTRIEESTPSDWQKVGAEFMPYVRQLPDRLMAHLKLLE
                     GDFGGFPVDRYTHSLQTATRALKAGRDEEYVVVALFHDIGDTLGSYNHFDIAAAILKP
                     FVSAENLWLVQNHGIFQGYYFFHHIGLDRNLRDQFRGHAHFERTAEFCDLYDNPAFDP
                     KAETLPMSEFEPMVRRVFAQPKNSIYMAPAGAFGVETTTV"
     gene            complement(1870..3963)
                     /locus_tag="VNU48_11225"
     CDS             complement(1870..3963)
                     /locus_tag="VNU48_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HWI11892.1"
                     /translation="MSANYELRGNVAVITLDNPPVNGLGHATRLGIVNGLDKALADAT
                     VTAIVITGAGKAFSGGADIREFGSPKAFAEPNLLSVILALEASTKPVVAAIHSVAMGG
                     GLELALGCHYRVAAKGVQVALPEVKIGLIPGAGATQRLPRVIGVENAMNMIVSGEAVK
                     SEVLAAAPGQKLFDKLAEGDLMTEALAFAGDVAGKRPLPLVRNLKVTHPNADAYVQFV
                     KNMVGGMAKNFPAPLKCVEAVAASMKLPFDAGMALERELFINLTLTPESKALRHAFVA
                     ERATTKIPDVPADTPIRDIKRVAVIGAGTMGGGIAMNFLNACIPVVMLEMKQEALDKG
                     LGIIRKNYDAQVKKGKLKQDKLDARMALLSTTLNYGDLKDADLVIEAVFEELGVKEAV
                     FKTLDEVMKPGAILASNTSTLDLNKIAAFTKRPQDVIGMHFFSPANVMKLLEVVRGEK
                     TAKDVLATVMAIGKKIKKTCVVSGVCDGFIGNRMVEQYARQAGFLLDEGASPAQVDKA
                     MEKFGMAMGPFRMGDLAGNDIGWYIRKRRNAEQPGMRYSKTADLLCEMGRFGQKTGAG
                     WYDYGPGKRDPIPSQVVTDMIEKHRKELGITPRKIGDDEIVGRLVYALVNEGAKIVEE
                     GIALRASDIDMVYLTGYGFPLHRGGPMNYADQVGLFNVVAAMKRFAANPLDDASFWTP
                     APLLAKLAAEGKVFN"
BASE COUNT          610 a         1404 c         1411 g          804 t
ORIGIN      
        1 gcgccgcggc gcttgccttc gatcagcgca tggccggtca gacgctggcc cgacataccg
       61 tacgggtgcc cgaccgcgat cgcgccgccg ttgacgttga gcttgtccat cgggatgccg
      121 agcgtgtcgg cgcagtacag cacctgcacc gcgaaggctt cgttcagttc ccagaggtcg
      181 atgtcggcga cggtcaggcc gagcttcttc agcaccttgg gcacggcgaa gaccgggccg
      241 atgcccatct cgtcgggctc gcaaccggcc accgcgaagc cgaggaagcg acccagcggc
      301 ttcaggttgc gcttcgacgc gagcgtttcg ctcatcagca cgcacgcgcc gccgccatcg
      361 ctgagctggc tcgcgttgcc ggccgcgacc acgccgccgg ggatcgcagt cttgatgccc
      421 atgatgcttt cgtaggtcgt gccggggcgg atgccttcat cggccttgat cgtcacttcc
      481 ttggtgcgca gcccctgcac cgggtcggca aagccggcca tcacggtgca ctcgatgatc
      541 tcgtcgctga acttgccggc ttcgagcgcg gccgtggccc tctgctggct cgcggcgccg
      601 taccggtcca tgcgctcgcg gccgatcttg tagcgcttgg cgacctgctc ggccgtctgc
      661 agcatgggcc agtagatctc gggcttgtgc tcggccagcc acgggtcgcc caacatgtgc
      721 ttgttgctct cgttccatac gcacgagatg ctctcgacgc cgcccgcaac ctgcacatcc
      781 gactcgccgg cgatgatgcg ctgcgcggcc aacgcgatgg tctgcaggcc gctggagcag
      841 aagcggttca ccgtcatggc actggtcgtg acgggcaggc cggcgcgcag cgcgacttgg
      901 cgcccgatgt tggtgccggt cgcaccttcg ggcatgccgc agcccatgat cacgtcgtcg
      961 acctcggcgc cgtcgatccc ggcgcgttcg accgccgcct tgacggcgag gccgccgagc
     1021 gtggcgccgt gcgtcatgtt gaaggcaccc ttccagctct tggccagggg tgtgcgggcg
     1081 gttgaaacga tcacggcgct ggtcatgact ctctcctgtg gatcaaacgg tggtggtttc
     1141 gacgccgaag gcgccggcgg gggccatgta gatgctgttt ttcggctgcg cgaacacgcg
     1201 ccgcaccatc ggctcgaact cgctcatcgg caaggtctcg gccttcgggt cgaacgccgg
     1261 gttgtcgtag agatcgcaga actcggccgt gcgctcgaag tgcgcgtggc cgcggaactg
     1321 gtcgcgcagg ttgcggtcga ggccgatgtg gtggaagaag tagtagcctt ggaagatgcc
     1381 gtggttctgc accagccaca ggttctcggc gctgacgaat ggcttcagga tcgccgcggc
     1441 gatgtcgaag tggttgtagg agccgagcgt gtcgccgatg tcgtggaaca gtgccaccac
     1501 cacgtactct tcgtcgcgcc ccgccttgag cgcgcgcgtg gcggtctgca gcgagtgcgt
     1561 gtagcggtcg accgggaagc cgccgaagtc gccctcgagc aacttcaggt gcgccatcag
     1621 gcgatcgggc aactggcgca cgtagggcat gaactcggcg ccgacctttt gccagtcgct
     1681 tggcgtgctt tcttcgatcc tcgtgaaggt cgctcggtcg ttcattgcgt gtgtcctcgt
     1741 catggcgcgg gcgccaagtt gctgatcaat gggtgatgga accggatcaa ctcaacgcag
     1801 ttgtcgctcg acatgcgttc gttgctgccg tgaaagcctt ccggtcttcc ccttgcgcac
     1861 gtcggccggt cagttgaaca ccttgccctc ggccgcgagc ttggccagca gcggcgcggg
     1921 cgtccagaac gacgcgtcgt cgagcgggtt cgccgcgaag cgcttcatcg ccgcgacgac
     1981 gttgaacagg ccgacctggt ccgcatagtt catcggcccg ccgcggtgca gcgggaagcc
     2041 gtagccggtg aggtagacca tgtcgatgtc ggaggcgcgc agcgcgatgc cctcctcgac
     2101 gatcttcgcg ccctcgttga cgagcgcgta gacgagccgg ccgacgatct cgtcgtcgcc
     2161 gatcttgcgc ggcgtgatgc ccagctcctt gcgatgcttc tcgatcatgt cggtgacgac
     2221 ctggctcggg atcgggtcgc gcttgcccgg cccgtagtcg taccagccgg cgccggtctt
     2281 ctggccgaag cggcccatct cgcacagcag gtcggcggtc ttcgaatagc gcatccccgg
     2341 ctgctcggcg ttgcggcgct tgcggatgta ccagccgatg tcgttgccgg ccaggtcgcc
     2401 catgcggaac gggcccatcg ccatgccgaa cttctccatc gccttgtcga cctgcgccgg
     2461 gctcgcccct tcgtcgagca gaaagccggc ctgacgcgcg tactgctcga ccatgcggtt
     2521 gccgatgaag ccgtcgcaca cgcccgagac gacgcaggtc ttcttgatct tcttgccgat
     2581 cgccatcacg gtggcgagca cgtccttcgc ggtcttctcg ccacgcacga cctcgagcag
     2641 cttcatcacg ttggccgggc tgaagaagtg catgccgatc acgtcctgcg ggcgcttcgt
     2701 gaacgcagcg atcttgttga ggtcgagcgt gctggtgttg ctggccagga tcgcgcccgg
     2761 cttcatgacc tcgtcgagcg tcttgaacac ggcctccttg acgcccagtt cctcgaacac
     2821 cgcttcgatc accaggtcgg cgtccttcag gtcgccgtag ttcagcgtcg tgctcagcag
     2881 cgccatgcgc gcgtcgagct tgtcctgctt cagcttgccc ttcttgacct gcgcgtcgta
     2941 gttcttgcgg atgatgccca ggcccttgtc gagcgcttcc tgcttcatct cgagcatcac
     3001 gaccggaatg caggcgttca ggaagttcat cgcgatgccg ccgcccatcg tgccggcgcc
     3061 gatcacggcg acgcgtttga tatcgcggat cggcgtgtcg gccggcacgt ccggaatctt
     3121 ggtggtcgcg cgctcggcga cgaacgcatg gcgcagcgcc ttgctctcgg gcgtgagcgt
     3181 caggttgatg aacagctcgc gctcgagcgc catgccggcg tcgaacggca gcttcatcga
     3241 agcggccacc gcctcgacgc acttcagcgg cgccgggaag ttcttggcca tgccgccgac
     3301 catgttcttg acgaactgca cataggcatc ggcgttcggg tgcgtgacct tcaggttgcg
     3361 caccaggggc aacggccgct tgccggccac gtcgccggcg aacgccagcg cctcggtcat
     3421 caggtcgccc tcggcgagct tgtcgaagag tttctggccc ggcgccgccg cgagcacctc
     3481 gctcttcacg gcctcgccgc tgacgatcat gttcatcgcg ttctcgaccc cgatcacgcg
     3541 cggcaggcgc tgcgtggcgc cggcgcccgg gatcagaccg atcttgacct cgggcaacgc
     3601 aacctgcacg cccttggcgg cgacgcggta gtggcaaccg agcgcgagct ccaggccgcc
     3661 gcccatggcc acgctgtgga tcgccgcgac gaccggcttg gtcgaggcct cgagcgcgag
     3721 gatcacgctg agcaggttcg gctcggcgaa cgccttgggg ctgccgaact cgcggatgtc
     3781 ggcgccgccc gaaaacgcct tgccggcgcc ggtgatcacg atcgccgtga cggtcgcgtc
     3841 ggccagcgcc ttgtccaggc cgttgacgat acccagccgt gtggcatgac cgaggccgtt
     3901 gacgggtggg ttgtccagcg tgatgacggc cacgttgccg cggagttcgt agttggcgct
     3961 catggggttc ctcggagggg gtcgggcaaa gaaaagaacg gtcgttcgat tctaggcgtt
     4021 gcaagaaaag ggctgctgtc tctgcggtga cagcgccttc ggtcgcgggc tgtacacgcg
     4081 tcggacttcg agccacgcct ctcgaaccac cgcgcattgc gcgacccgca acgcatgaag
     4141 cgctgcggtg gcttgggatg tctggttgcg gcccgttgcg gtcgttcgca agcgagcgtg
     4201 cgccctttga cggtcggcgc gagccgaga
//
LOCUS       DATMJB010000521         3202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_270753, whole genome shotgun sequence.
ACCESSION   DATMJB010000521 DATMJB010000000
VERSION     DATMJB010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270753"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VNU48_11230"
     CDS             complement(<1..1089)
                     /locus_tag="VNU48_11230"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HWI11893.1"
                     /translation="MSKPLTFIDAFAGCGGLSLGLMQAGLTGKFAIEHDSFAFATLKA
                     NLLSKASPFKYSWPRWLPKNPLSIASLLGEYAQELAALNGGVDVLVGGPPCQGFSMAG
                     RRQHDDPRNQLFAQYLRLVEIVKPKIVLIENVRGFTMDFAVGENVTNYADALKAVLSG
                     DYKVHERLLDLSQFGVPQARTRYFVIAVRSTVSTTDPFKLLESRVPTFLRALGISAPV
                     SSSNAISDLEVGRCGTEPSTDTKGFDQIAYGRPLTRYQRLMNEAGVTPSDLRLARHAD
                     DIEQRFREIIEVSHADGRLNTSISAEVRERFGLKKKALRVLDPDRPSPTITSMPDDLL
                     HYSEPRTLTVRENARLQSFPDWYAFQGKY"
     gene            complement(1279..1722)
                     /locus_tag="VNU48_11235"
     CDS             complement(1279..1722)
                     /locus_tag="VNU48_11235"
                     /inference="COORDINATES: protein motif:HMM:NF019238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HWI11894.1"
                     /translation="MSPFDTLDEARRTLARLPGAPLRSTGAWDVPQVLHHVAQSVEYS
                     LDGFPQLKPAWFRATLGRLAFAIFSARGRMSHALDAPIPGAPPIASGLPLTAAVAHVL
                     AALDRFERHDGPLQPHFAYGALDKAAFGRAHLMHLNDHWGEFVAG"
     gene            1860..>3202
                     /locus_tag="VNU48_11240"
     CDS             1860..>3202
                     /locus_tag="VNU48_11240"
                     /inference="COORDINATES: protein motif:HMM:NF015083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HWI11895.1"
                     /translation="MTAVLLIAASLLAGCTAFDGTLADTDDCAKMLAGGLRPLADERM
                     DRFVGKVGEPVARCRGGDRAAQWRGTPYVDWANYWATGDAASFAPGTRSLGSGLAGHL
                     GANGRGIDGALLDLEYQRMELVKFNLFDNAGTYAAFVTGRDGRPGPTLKSWPEMRLLK
                     GAPDYAAVGGDGPQLCSGELIRFRKLDGSCNDLRNPRMGSTGTPFARNVQFEATYPEL
                     GNDELARNRHGDRLGLLKPDPQLISRLLFTRAQSDASKCRDGEGLPGNSPAANCDYQK
                     APFFNVLAAFWIQFMTHDWFSHMEEGHNEPVWMPTGCTTPQARALGCRDGDRIDRAYV
                     ADASAPPRVAASDGEARIARAYKTHANNVTAWWDASQIYGFDATSRQRVKRDPADPAR
                     LLLRPGPHAGAGEAQGELPVFDAGDPILPQWAGQGAAAFPDNWSIGVAFYHTLFAR"
BASE COUNT          560 a         1087 c         1052 g          503 t
ORIGIN      
        1 gtatttgccc tggaacgcat accagtccgg gaagctctgc aaacgggcgt tctcacgcac
       61 cgtgagtgtc ctaggttcgc tgtagtgaag aaggtcgtcc ggcatgcttg taatggtcgg
      121 cgacggacga tcgggatcga gcacgcgcaa tgccttcttc tttagtccga accgctcacg
      181 gacttcggcg ctgatgctcg tgttgagccg cccgtccgca tggctgactt ctatgatctc
      241 tcggaagcgc tgttcgatgt cgtccgcatg gcgggcgagg cgaaggtcgc tgggcgtcac
      301 gccggcttca ttcatgagtc tttgataccg cgtcagcggc cggccgtagg caatctgatc
      361 gaatcccttc gtgtctgtgg acggttccgt gccgcagcgg ccgacttcga gatcgctgat
      421 tgcattcgac gacgatacgg gcgcactgat gcccagggcg cgaaggaatg tgggaacccg
      481 gctttcgagt agcttgaatg ggtcggtggt tgacacagtg gagcggacag caatcacgaa
      541 gtagcgtgta cgcgcctgtg gcacgccaaa ctgcgataga tcgagcagcc gctcatgcac
      601 cttgtagtcg ccggatagca cggccttcaa ggcgtcggca tagttggtca cgttctcccc
      661 gaccgcaaag tccatcgtga agccgcgcac attctcgata agaacgatct tcggcttcac
      721 gatctcaaca agccggagat actgggcgaa aagttggttg cgagggtcgt catgctgtct
      781 gcggccggcc attgaaaatc cctggcacgg cgggccgccg actaaaacat caacaccgcc
      841 attgagcgcc gccagctcct gggcgtattc gcctagcagt gacgcaatgc tcagcgggtt
      901 tttgggcaac caccgcggcc atgaatactt gaacggcgac gccttcgaca gcagattggc
      961 cttcaacgtc gcgaaggcga aactgtcgtg ctcaatggca aatttgcccg tcaggccggc
     1021 ctgcattaac ccaagcgaaa gccctccgca cccggcaaaa gcgtcaatga aggtaagtgg
     1081 cttggacatt ctcgctttct ctcagaatac ttttgattgt gcggttctta tgaagaatgc
     1141 accatgcgca aacttctacc ggcgggccga aagcggcggc ctgttgactg tacgcgacac
     1201 gcctaaacag gacgcaagca ccccacggca cgcagcgaca gccaaatggc agcgtgaagg
     1261 cgttggggtg ccaaccgctc accccgccac aaactccccc cagtgatcat tcaagtgcat
     1321 caggtgcgcc cgcccaaacg ccgccttgtc cagcgcccca tacgcgaagt gcggctgcag
     1381 cggcccgtcg tgccgctcga atcgatccag cgccgcgagc acatgcgcga ccgccgccgt
     1441 cagcggcaac cctgacgcga tcggcggcgc gccggggatc ggcgcatcga gcgcgtgcga
     1501 catccgcccg cgcgccgaga agatcgcgaa cgcgagccgg ccgagcgtcg cgcggaacca
     1561 cgccggcttg agttgcggga agccgtcgag cgagtactcg acgctttgcg cgacatggtg
     1621 cagcacctgc ggcacgtccc acgcgccggt gctgcgcagc ggcgcgcccg gcagccgcgc
     1681 cagcgtgcgc cgggcttcgt cgagggtgtc gaacgggctc accgcggcag tgtgccagca
     1741 gcgtaggttc gattcttggt gttgacgacc accggtacgc tgcctacctt cgcagcctcg
     1801 acccaaagag acgggcgcag gagacgacga tgaccccacg acggtccgct cggaagttga
     1861 tgacggccgt gctgctgatc gcagcgtcgc tgctcgctgg ctgcaccgcg ttcgacggca
     1921 cgctggccga caccgacgac tgcgcgaaga tgctcgccgg cggcctgcgc ccgctcgcgg
     1981 acgagcgcat ggatcgcttc gtcggcaagg tcggcgagcc ggtggcgcgc tgccgcggcg
     2041 gcgatcgcgc cgcgcaatgg cgcggcacgc cgtacgtcga ctgggccaac tactgggcca
     2101 ccggcgacgc cgcgagcttc gcgcccggca cgcgctcgct cggcagcggg ctggccggcc
     2161 acctgggcgc gaacggtcgc ggcatcgacg gcgcgctgct cgacctcgag taccagcgca
     2221 tggagctggt caagttcaac ctgttcgaca acgccggcac ctacgccgcg ttcgtcaccg
     2281 gccgcgacgg ccggcccggc ccgacgctca agagctggcc cgagatgcgg ctgctcaagg
     2341 gcgcgcccga ctacgccgcg gtcggcggcg acgggccgca gctgtgcagc ggtgagctga
     2401 tccgcttccg caagctcgac ggcagctgca acgacctgcg caacccgcgc atgggctcga
     2461 ccggcacgcc gttcgcgcgc aacgtgcagt tcgaggccac ctaccccgag ctcggcaacg
     2521 acgaactcgc gcgcaaccgc cacggcgacc ggctcggctt gctgaagccc gacccgcaac
     2581 tgatcagccg gctgctgttc acgcgagcgc agagcgacgc gtcgaagtgc cgcgacggcg
     2641 aaggcctgcc gggcaactcg ccggccgcga actgcgacta ccagaaggcg ccgttcttca
     2701 acgtgctcgc ggcgttctgg atccagttca tgacgcacga ctggttctcg cacatggagg
     2761 aaggccacaa cgagccggtc tggatgccca ccggctgcac cacgccgcag gcgcgcgcgc
     2821 tcggctgccg cgacggcgac cggatcgacc gcgcctacgt ggccgatgcg agcgcgccgc
     2881 cgcgcgtggc ggcgagcgac ggcgaggcgc gcatcgcgcg cgcctacaag acgcacgcca
     2941 acaacgtcac cgcgtggtgg gacgcgtcgc agatctacgg ctttgatgcg acctcgcgcc
     3001 agcgcgtcaa gcgcgacccg gccgatcccg cgcggctgct gctgcgcccg gggccgcacg
     3061 ccggcgccgg cgaggcgcag ggcgagctgc cggtgttcga cgccggcgac ccgatcctgc
     3121 cgcagtgggc cggccagggc gcggcggcgt ttcccgacaa ctggagcatc ggggtcgcct
     3181 tctatcacac gctgttcgcg cg
//
LOCUS       DATMJB010000522         2454 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_690434, whole genome shotgun sequence.
ACCESSION   DATMJB010000522 DATMJB010000000
VERSION     DATMJB010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690434"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..817
                     /locus_tag="VNU48_11245"
     CDS             167..817
                     /locus_tag="VNU48_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWI11896.1"
                     /translation="MKKLNKVASLFATAALATSMSGAFAQTVDNWVSGQGSLPWKNGT
                     NELCWRDGSWTPATAIAECDGALKPPPPPAPAPPPPPAPPPKPAPAPVVVPAATSEKV
                     TFAADAFFDFDKSVLKAEGKAKLDDLVSKMGGINLEVIIAVGHTDSVGADAYNQKLSV
                     RRSEAVKAYLVSKGVEKNRVYTEGKGEKQPVADNKTAEGRAKNRRVEIEVVGTRGK"
     gene            906..1607
                     /gene="ubiG"
                     /locus_tag="VNU48_11250"
     CDS             906..1607
                     /gene="ubiG"
                     /locus_tag="VNU48_11250"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HWI11897.1"
                     /translation="MINADPQELAKFSELAHRWWDTESEFRPLHQINPLRLDWIDRHA
                     KLAGTRVLDVGCGGGILSDSMARRGATVLGIDLAAKPLKVAQLHALEAGTENVEYREV
                     AAEALAAEAPAAFDTVTCMEMLEHVPDPAAIVRACATLAKPGGWVFFSTINRNAKAFL
                     FAIVGAEHVLQLLPKGTHEYARFIRPSELAQWCRNAGLELAHTRGMEYNPITRRYWFS
                     ADTSVNYLIACRKPA"
     gene            1604..2278
                     /gene="gph"
                     /locus_tag="VNU48_11255"
     CDS             1604..2278
                     /gene="gph"
                     /locus_tag="VNU48_11255"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345990.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HWI11898.1"
                     /translation="MTTTPGTIRAVLFDLDGTLIDSAPDLAGAGNDMRIARGLAPHSL
                     AHYRPMVGAGARGMVGRAFDVGPQDDAFAALRDEFLQRYEARMTQQTRVFPEMLPVLA
                     ALDAGAMPWGIVTNKATRFSAPLVHALRLMPPAAALVCGDTTPHAKPHPEPLLEAARR
                     LGVPASGCVYVGDDLRDVQAGRAAGMMTVAVLWGYLGAGESVDGWGADHLAQTPSDLL
                     KLLRMT"
     gene            2291..>2454
                     /gene="ssrA"
                     /locus_tag="VNU48_11260"
     tmRNA           2291..>2454
                     /gene="ssrA"
                     /locus_tag="VNU48_11260"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          423 a          849 c          801 g          381 t
ORIGIN      
        1 gatgccgcac gggcgtggag tgtgtccaaa ttcaggctta gcgaaagccc tatggcacaa
       61 tcgccccggt ggcaagctac gcgaaagcga aggtttgcca gtgctttccg attccagttc
      121 ggacgttttg cagaaactaa ctgcaccttc cctcgagagg agaatcatga agaaactgaa
      181 caaggtggcg tcgctctttg caaccgccgc cctcgcaact tcgatgtctg gcgcgtttgc
      241 gcagacggtc gacaactggg tcagtggcca aggcagcctg ccctggaaga acggcaccaa
      301 cgaactgtgc tggcgtgatg gctcctggac tcctgccacc gcgatcgccg agtgcgacgg
      361 cgcgctgaag ccgccgccgc cgcccgctcc ggctcccccg ccgccgcccg ccccgccgcc
      421 gaagcccgca ccggccccgg tggtcgtgcc cgccgcaacg tccgagaagg tgaccttcgc
      481 cgccgatgca ttcttcgact tcgacaagtc ggtgctcaag gctgagggca aggccaagct
      541 ggacgatctg gtcagcaaga tgggcggcat caacctcgag gtgatcatcg ccgtcggcca
      601 caccgactcg gtcggtgcgg atgcctacaa ccagaagctg tcggtgcgtc gctccgaagc
      661 cgtcaaggcc tatctggtct cgaagggcgt cgagaagaac cgcgtctaca ccgaaggcaa
      721 gggcgagaag cagccggtcg ccgacaacaa gaccgccgaa ggccgcgcga agaaccgtcg
      781 cgtggaaatc gaagtcgtgg gcacccgcgg caagtgatcg ccgcgcggcg ggcctcgcgg
      841 tccgctgatg cacaaaaccc cgcttcggcg gggttttctc tttgtgcatt cgataacatc
      901 gccgcatgat caacgccgac ccgcaagagc ttgccaagtt cagcgaactc gctcaccggt
      961 ggtgggacac cgagagcgag ttccgacccc tgcaccagat caacccgctc cggctggact
     1021 ggatcgaccg gcacgcgaag ctggccggca cacgcgtgct cgatgtcggc tgtggcggcg
     1081 gcatcctgtc ggactcgatg gcgcggcgcg gcgccacggt gctcggcatc gatctggccg
     1141 cgaagccgct gaaggtcgcg cagctgcatg cgctcgaggc cggcaccgag aacgtcgagt
     1201 accgcgaggt cgcggccgag gccctcgccg ccgaagcccc ggccgccttc gacaccgtca
     1261 cctgcatgga gatgctcgag cacgtgcccg acccggccgc gatcgtgcgc gcctgcgcga
     1321 ccttggccaa gcccggcggc tgggtcttct tctcgacgat caaccgcaat gccaaggcct
     1381 tcctgttcgc gatcgtcggt gccgagcatg tgctgcagtt gctacccaag ggcacgcacg
     1441 agtacgcgcg tttcatccgt cccagcgaac tcgcacagtg gtgccgcaac gccggcctcg
     1501 aactcgccca cacgcgcggc atggaataca accccatcac gcggcgctac tggttctcgg
     1561 ccgacaccag cgtcaactac ctgatcgcgt gtcgcaagcc ggcatgacga cgacgcccgg
     1621 gacgattcgc gccgtgctgt tcgatctgga cggcaccttg atcgacagcg cccccgacct
     1681 cgccggcgcc ggcaacgaca tgcgcatcgc gcgcggcctg gcgccgcact cgctggcgca
     1741 ctatcggccg atggtgggtg ccggcgcgcg tggcatggtc ggccgcgcgt tcgacgtcgg
     1801 cccgcaggac gacgccttcg cggcgctgcg agacgagttc ctgcagcgct acgaggcccg
     1861 catgacgcag cagacccgcg tgttccccga gatgctgccg gtgctggccg cgctcgacgc
     1921 cggcgccatg ccctggggca tcgtcaccaa caaggcgacg cgcttcagcg cgccgctggt
     1981 gcacgcgctg cggctgatgc cgccggccgc ggcgctggtg tgcggggaca ccacgccgca
     2041 cgccaagccg caccccgagc cgctgctcga ggcggcgcgc cgcctcggcg tgcccgcgtc
     2101 cggctgcgtc tacgtgggcg acgacctgcg cgacgtgcag gccggccgcg cggccggcat
     2161 gatgacggtg gcggtgctgt ggggctacct cggcgccggc gaatcggtcg atgggtgggg
     2221 tgccgatcat ctggcgcaaa cgccgagcga tctcttgaag ctgctgcgaa tgacctaaac
     2281 tagtcgttct ggggctgacc tggcttcgac gaaggttcgg agtcggaaca gtgcacgccg
     2341 agcaccagtt cgctcgtaaa tccactggaa acaaagtaac tgcgaacgac tcttcgtacg
     2401 ccctcgccgc ttaaaaccgg tgagcctcgc aacagttggc cgatgggctg ggtc
//
LOCUS       DATMJB010000523         9471 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_257408, whole genome shotgun sequence.
ACCESSION   DATMJB010000523 DATMJB010000000
VERSION     DATMJB010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9471
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257408"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..941)
                     /locus_tag="VNU48_11265"
     CDS             complement(12..941)
                     /locus_tag="VNU48_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HWI11899.1"
                     /translation="MKLLFDSFWRAVAYCLHPRVIMLSLLPLVLMAGLALGLGYFFWE
                     PAIDAVRASFDSWAVLKTLTGWLDGVGLANLKTVLAPLVVVFLSTPLIVVLSLLLVAA
                     FMTPAMLTLVASRRFPLLERKHGGSLVGSSFGALWATVAALLALLVSIPLWFVPPLVL
                     IVPPLIWGWLTYRVMSYDVLAEHASADERRALVRRHRTTLLGMGVLTGYLGAAPSLVW
                     ASGALAIVFAPVLVPAAVWIYTLVFAFAALWFAHFALAALQALRREREPVEILPPVRG
                     GMPEAELITDARLIPLPTSDRGGAPPSPSGSRP"
     gene            complement(1030..2175)
                     /locus_tag="VNU48_11270"
     CDS             complement(1030..2175)
                     /locus_tag="VNU48_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HWI11900.1"
                     /translation="MPPVVDARNLYSETAAGKIAPALRDDLERIYVPNLRSNDVSVVD
                     PATMKVVERLKVGVSPQHIVPAWDLRTLWVTNNAEGRTDGSLTPIDPRSGKTGKPIPV
                     DDPYNMYFTPDGKSAIVVAEARKRLDFRDPQTMAPQFSIATPGCPGINHADFSIDGRF
                     AIFTCEFSGSLAKIDLVNRKVLGYLQLQMPATRFKESSAPRAAPGKVWEPGESEICTV
                     AKGMPQDIRSSPDGKHFFVADMHADGVHIVDVESFTKVGFIATGLGAHGLYPSRDGKS
                     LYVANRGSHKIHGARLGDGGVTVIDFASEKIVARWGIPGGGSPDMGNVSADGKWLWLA
                     GRYDDVIYRFDTGSGAVQQVKVGAEPHGLTVWPQPGRYSLGHTGNLR"
     gene            complement(2359..3222)
                     /locus_tag="VNU48_11275"
     CDS             complement(2359..3222)
                     /locus_tag="VNU48_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HWI11901.1"
                     /translation="MLFRHSFCRPLASALMGVVVVLSTPWAPALAAPLPANPFIEEMT
                     WTELRDRIAAGATTVLVPIGGTEQSGPHMVLGKHNVRARVLAGRIAEQLGNAVVAPVI
                     AYVPEGAIAPPTAHMRFAGTISIPDAVFEAVLEATARSFKQHGFRDVVFLGDHGGYQQ
                     DEERVAARLNREWAAQPACRVHALVDYYRVTQTAYVESLKARGFTPDEIGTHAGLADT
                     ALALAVDPALVRQELLTRGPKAGVRDGVQGDPRRATAELGRTGVERIVEVSVAKLREL
                     TRARAGNGKNE"
     gene            complement(3251..4081)
                     /locus_tag="VNU48_11280"
     CDS             complement(3251..4081)
                     /locus_tag="VNU48_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWI11902.1"
                     /translation="MMADAAQADSDKVFSGSVPQLYDEYLVPLIFEPYALDLARRVAA
                     LTPARVLEIAAGTGVVTRQLAIALEPDAAIVATDLNQPMLDRAAAVGTRRAVEWRQAD
                     AMQLPFDDAVFDVVVCQFGVMFFPDKAHAFAQARRVLRPGGVLVFNAWDRIEDNEFAA
                     TVTAALATMFPNDPPRFMARTPHGYHDPGAIAADLRAGGFAAPPDIATVTARSRADSA
                     RTVAVAYCQGTPLRNEIETRDKARLGEATDIAAAALARRFGTGPVDGKIQAHVLTVAR
                     "
     gene            complement(4132..4560)
                     /locus_tag="VNU48_11285"
     CDS             complement(4132..4560)
                     /locus_tag="VNU48_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HWI11903.1"
                     /translation="MSILWKQTMSVEALTASNAGTAAAHLGVEFLEVGDDFIRGRVPV
                     DSRTRQPMGILHGGVSVVLAETLGSCGAACAAPAGRRVVGLDINANHLRSVSEGWVTG
                     TARPIHVGRSTHVWQIDMVNDQGQLTCVSRITMAILSPAA"
     gene            complement(4733..6106)
                     /locus_tag="VNU48_11290"
     CDS             complement(4733..6106)
                     /locus_tag="VNU48_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWI11904.1"
                     /translation="MLKIHNPATGALIAEVPEATPEIVAERTRAARAAQPAWGAQPMA
                     QRLQMLQRFRVALAAELETLAVTLTAEVGKPIRQSRSEINGLLVRIDFFLAQAETTLA
                     TQQVADDAGTRELISFDPLGVIANISAWNYPYFVGGNVFVPALLAGNAVLYKPSEFAT
                     LTGLNIARLLHESGVPSEVFVPLVGGAAVGAALLEQRIDGVFFTGSHATGARIAQTVG
                     SRFIKLQLELGGKDPTYVADDADVRVAAESLADGAMYNTGQSCCSVERIYVHERVHDA
                     FVAAFVETVDGMRLGDPRSEDTTIGAITRAPQLDVLEAQVADAQAKGATLRIGGKRLV
                     DRPGHWFEPTVFTEVDHTMTLMREESFGPVVGIQKVASDADALTLMNDTRYGLTAGVF
                     TKSEDRARAILSQVNAGTVYWNCCDRVSPRLPWSGHGDSGVGLTLSTLGIQTFTRPRA
                     WHLRQSA"
     gene            complement(6138..6938)
                     /locus_tag="VNU48_11295"
     CDS             complement(6138..6938)
                     /locus_tag="VNU48_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828739.1"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HWI11905.1"
                     /translation="MARPLIIGISARIHHPVGPVLNLGGVYTKTLHYLEQSVAHWVLA
                     KDVLAVMIPAIESEGLIQRGDMSLASYAEHLDGLVLQGGADVAPESYGETPLEPAWAG
                     DRVRDRYEIDLFNAFVAAGKPVIGICRGCQLINVALGGTLFQDIPTQVTQAISHRDAD
                     LYERALHDVTLVRGTRLSQMYPDVQQAKVNSIHHQAVKDLGRDMVVEALGVPDGIVEA
                     IRWRGPSYVFGMQWHPEFLALKSLDQAQLDGGPILADFLATARAARDA"
     gene            complement(6938..8077)
                     /locus_tag="VNU48_11300"
     CDS             complement(6938..8077)
                     /locus_tag="VNU48_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWI11906.1"
                     /translation="MAITRFSFPTTIHFGPGASALAGPHLNERGCKRPLIVTDKGLAA
                     LPLIAELKAALEAAGLKPAVYAGVFGNPTGPQVMDGAAAYKAHEADAVVGVGGGAALD
                     VAKVVGLLATHPGDVMEYAWDHPQVRAIDAAALPYFIALPTTAGTGSEVGRSSVISEE
                     ATHVKRVIFSPAILAKVVFADPELTLGLPPAVTAATGMDALTHNIESYLSPAYHPLCD
                     GIALEGVRIAARSLLTAVRDGADLAARADMMMSSMMGAIAFQKDLGAVHSCAHALGTV
                     CDLHHGLANALMIEPVMSYNREAVPHKFAELAHAVGVPSVSNFVPWLAQLKHRIGIPP
                     GLALVGVKREHFPRLVEVATADICHQTNPRPCEAADFARFFDEAF"
     gene            complement(8114..>9471)
                     /locus_tag="VNU48_11305"
     CDS             complement(8114..>9471)
                     /locus_tag="VNU48_11305"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HWI11907.1"
                     /translation="HPALKAVRASGTCKVKVAVSDIDGILRGKYLHIDKFLGAAQPQP
                     NGGFGFCSVVFGWDAGDNCYDNTKLTGWQHGYPDALARLDLDTYRTVPWDDNVPFFLG
                     DFVTADGAPSPICPRQTLKRVLKRAEKLGVQALTGMEFEWFNFAETPQSWAAKNGIAP
                     EPITPGMFGYSLLRMNHKREFFKALMDEMPLFGVPIEGLHTETGPGVYEVAIAFGAAL
                     EQADRAILFKTGAKEIGARFGVMPSFMAKWSQQYPGCSGHIHQSLSDGKNNLFFDAKS
                     PRRMSKLFESYLAGQVGFLMEMAPMFWPTINSYKRLVDGFWAPVKPTWGMDNRTASFR
                     VIAGSPKATRLETRCPGADVNPYLAMAAVIAAGLHGVEQGLKLSAPPITGTNEGAEHI
                     PRAPRTLIETTRVFKQSAVARDWFGDDFVDHFAATREWEWRQWLDGVTDWELKRYFEI
                     I"
BASE COUNT         1425 a         3283 c         3325 g         1438 t
ORIGIN      
        1 cacgccgaag ttcatgggcg gctcccggat gggctcggtg gcgcgccgcc ccgatcgctg
       61 gtcggcagcg ggatcaggcg cgcgtccgtt atcagttcgg cttccggcat gccgccgcgc
      121 accggcggca ggatctcgac cggctcgcgc tcgcgccgca aggcctgcag cgcggcgagc
      181 gcgaaatgcg cgaaccacag cgccgcgaac gcgaacacca gcgtatagat ccacaccgcc
      241 gccggcacca gcaccggcgc gaacacgatc gccagcgcgc ccgaggccca caccagcgat
      301 ggcgccgcgc cgaggtagcc ggtgagcacg cccatgccga gcagcgtcgt gcggtgccgc
      361 cgcacgagcg cgcggcgctc gtcggcactg gcgtgttcgg caagcacgtc gtagctcatc
      421 acgcggtagg tcagccaacc ccagatcagc ggcggcacga tcagcaccag cggcggcacg
      481 aaccacagcg ggatcgacac cagcagcgcc agcagcgccg cgaccgtggc ccagagcgcg
      541 ccgaagctgc tgcccacgag cgagccgcca tgcttgcgct cgagcagcgg gaagcgccgg
      601 ctcgcgacca gggtcagcat cgccggcgtc atgaacgccg ccaccagcag cagcgagagc
      661 acgacgatca gcggcgtcga gaggaacacc acgaccagcg gcgcgagcac ggtcttcagg
      721 ttcgccaggc ccacgccgtc gagccagccg gtcagcgtct tcaggaccgc ccacgagtcg
      781 aagctggcgc gcactgcatc gatcgccggc tcccagaaga agtagcccag gccgagcgcc
      841 aggccggcca tcagcaccag cggcagcagc gacagcatga tcacgcgcgg gtgcaggcag
      901 taggcgaccg cgcgccagaa cgagtcgaac agcagcttca ctggagcccc tcgtcgaatg
      961 agtacattcg ccgatccccc ggggggatgc gggccggctc gggagcggcc cagcgctcgg
     1021 cccgcatcat cagcgcaggt tgccggtatg cccgagcgaa tagcgcccgg gctgcggcca
     1081 caccgtcagc ccatgcggct cggcaccgac cttgacctgc tggaccgcgc cgctgccggt
     1141 gtcgaagcgg tagatcacgt cgtcgtagcg gccggccagc cacagccact tgccgtcggc
     1201 gctgacgttg cccatgtcgg ggctgccgcc gcccggtata ccccagcgcg cgacgatctt
     1261 ctcggaggcg aagtcgatca ccgtgacgcc gccgtcgccg agccgcgcgc cgtggatctt
     1321 gtgcgagccg cggttcgcga cgtacaggct cttgccgtca cggctcgggt acaggccgtg
     1381 cgcgccgaga ccggtggcga tgaagccgac cttggtgaac gattcgacat cgacgatgtg
     1441 cacgccatcg gcatgcatgt cggccacaaa gaagtgcttg ccgtcggggg agctgcggat
     1501 gtcctgcggc atgcccttcg cgaccgtgca gatctcggac tcgccgggtt cccagacctt
     1561 gccgggcgcg gcgcgcggcg cgctcgattc cttgaagcgc gtggccggca tctgcagttg
     1621 caggtagccc agcaccttgc ggttcaccag gtcgatcttc gcgaggctgc ccgagaactc
     1681 gcaggtgaag atcgcgaagc gcccgtcgat cgagaagtcg gcgtggttga tgccggggca
     1741 gcccggcgtg gcgatcgaga actgcggcgc catcgtctgc ggatcgcgga agtccaggcg
     1801 cttgcgcgcc tcggcgacga cgatcgccga cttgccgtcg ggcgtgaagt acatgttgta
     1861 cgggtcgtcg accgggatcg gcttgccggt cttgccgctg cgcgggtcga tcggcgtcag
     1921 gctgccgtcg gtgcggcctt cggcattgtt ggtgacccac agggtgcgca ggtcccaggc
     1981 cggcacgatg tgctgcgggc tcacgccgac cttcaggcgc tcgaccacct tcatcgtcgc
     2041 cgggtcgacc accgagacat cgttcgagcg caggttgggc acgtagatgc gctcgaggtc
     2101 gtcgcgcagc gccggcgcga tcttgcccgc cgcggtctcg ctgtacaggt tgcgtgcgtc
     2161 gaccaccggc ggcatgccgg gcagcgtcgg tatcgccggc gcaacggcgg cactcgccgc
     2221 cgcgggcggc ggcgcgttgg cggcgccctg cacgcgctcg gcggccgtca cgatcaggcc
     2281 cgccagcgcc atcagcgcca gcccggccgc ccgcttcttc tcaatcttca atcgattcat
     2341 ctgcaccgtt gggtcccatc actcgttctt gccattgcct gcacgcgccc gcgtcagttc
     2401 gcgcagcttc gccaccgaca cctcgacgat gcgctcgaca ccggtgcggc ccagttcggc
     2461 cgtcgcgcgg cgcggatcgc cctgcacgcc atcgcgcacg ccggccttcg ggccgcgcgt
     2521 cagaagctcc tgccgcacca gggccggatc gaccgcgagc gccagcgccg tgtccgccag
     2581 accggcatgg gtgccgatct cgtccggcgt gaatccgcgc gccttcagcg actcgacgta
     2641 ggcggtctgg gtgacgcgat agtagtcgac cagcgcatgc acgcggcacg ccggctgcgc
     2701 cgcccattcg cggttcaggc gcgccgcgac gcgctcctcg tcctgctggt agccgccgtg
     2761 gtcgccgagg aacaccacgt cgcgaaaacc gtgctgcttg aagctgcgcg cggtcgcttc
     2821 gagcacggcc tcgaagaccg cgtcggggat cgagatcgtg ccggcaaagc gcatgtgcgc
     2881 ggtcggtggc gcgatcgcgc cttccggcac ataggcgatc accggcgcca ccaccgcgtt
     2941 gccgagttgc tcggcgatgc gcccggccag cacgcgtgcg cgcacgttgt gcttgcccag
     3001 caccatgtgc ggcccgctct gctcggtacc gccgatcggc accagcaccg tcgtcgcgcc
     3061 ggccgcgatg cggtcgcgca attcggtcca ggtcatctct tcgatgaacg ggttggcggg
     3121 caagggtgcg gccagcgctg gcgcccacgg cgtcgaaaga acgacgacga cacccatcag
     3181 cgcgctggcc agcgggcggc agaacgaatg acgaaacagc atgtaaatca cgttcggggc
     3241 ggccggcgtt tcagcgtgcc acagtgagca cgtgcgcctg gatcttgccg tcgacgggac
     3301 cggtgccgaa gcgccgggcg agtgccgccg cggcgatgtc ggtggcttcg ccgagccgtg
     3361 ccttgtcgcg cgtctcgatc tcgttgcgca gcggcgtgcc ctggcagtag gcgaccgcca
     3421 cggtgcgtgc cgagtcggcc cggctgcgcg cggtcacggt ggcgatgtcg ggcggcgcag
     3481 cgaatccgcc ggctcgcaga tcggcggcga tggcgccggg gtcgtgatag ccgtgcggcg
     3541 tgcgtgccat gaagcgcggc gggtcattcg ggaacatggt cgcgagcgcg gccgtcacgg
     3601 tggccgcgaa ttcgttgtcc tcgatccggt cccaggcatt gaagaccagc acgccccccg
     3661 gccgcaacac ccggcgcgcc tgcgcgaacg cgtgcgcctt gtcggggaag aacatcacgc
     3721 cgaactggca cacgaccacg tcgaacaccg cgtcgtcgaa cggcagctgc atcgcgtcgg
     3781 cctggcgcca ctcgacggcg cgccgggtgc cgaccgcggc ggcgcggtcc agcatcggct
     3841 ggttcaggtc ggtcgcgacg atcgccgcgt ccggctccag cgcgatcgcc aactggcgcg
     3901 tgacgacgcc ggtgccggcg gcgatctcga gcacacgcgc cggcgtcaac gcggcaacgc
     3961 gccgcgccag atccagcgca tagggctcga agatcagcgg gacgaggtac tcgtcgtaga
     4021 gctgcgggac cgagccgctg aagaccttgt cgctgtcggc ctgcgccgcg tcggccatca
     4081 tcgtctcctc ctcgtggtgg tcatgggatc ccggaccaag acgccggtgg gtcaggcggc
     4141 cggcgagagg atcgccatcg tgatcctgga cacgcaggtg agttgcccct ggtcgttgac
     4201 catgtcgatc tgccagacgt gggtgctgcg cccgacgtgg atcgggcgcg ccgtgcccgt
     4261 gacccagccc tcgctgacgc tgcgcagatg gttcgcattg atgtcgagcc ccaccacccg
     4321 gcggcccgcc ggcgcggcgc atgcggcgcc gcaggagccg agcgtctcgg ccagcaccac
     4381 cgacacgccg ccatgcagga tgcccatcgg ctggcgcgtg cgtgaatcga ccggcaccct
     4441 gccgcggatg aagtcatcgc cgacctcgag aaattcgacg ccgagatggg ccgccgcggt
     4501 gccggcattg gacgcggtca gcgcctcgac cgacatggtc tgtttccaga gaatggacat
     4561 aaggggatcg actccttgga gtaaggcaga aggggttgcc gccagcgatt ctgacccgat
     4621 tccagaaagt cgcaggcccc atgcgccagc gcgcgcacgc cggggtcgga agcgctggcc
     4681 gtcatcgtgc ttcctctcgt tcgtggaatg tcgaacaacg ccagtcgcgt catcaggctg
     4741 attggcgcag atgccaggcg cgcggacgcg tgaaggtctg gatgcccagc gtcgacaagg
     4801 tcagcccgac gcccgaatcg ccgtgccccg accacggcag gcgcgggctg acgcggtcgc
     4861 agcagttcca gtagacggtg ccggcgttga cctgcgacag gatcgcgcgc gcgcgatcct
     4921 cgctcttcgt gaacacaccg gccgtcagcc cgtagcgcgt gtcgttcatc agcgtcagcg
     4981 catcggcgtc gctcgcgacc ttctggatgc cgacgaccgg gccgaagctc tcctcgcgca
     5041 tcaaggtcat cgtgtggtcg acctcggtga acacggtcgg ctcgaaccag tggccgggtc
     5101 ggtcgacgag ccgcttgccg ccgatgcgca aggtcgcgcc cttcgcctgc gcgtcggcca
     5161 cctgcgcctc gagcacatcg agttgcggcg cgcgcgtgat cgcgccgatg gtggtgtcct
     5221 cgctgcgcgg atcgccgagg cgcatgccgt cgacggtctc gacgaaggcc gcgacgaagg
     5281 cgtcgtggac gcgctcgtgc acgtagatgc gctcgaccga gcagcagctc tggccggtgt
     5341 tgtacatcgc gccgtcggcc agcgactcgg cggccacgcg cacgtcggca tcgtcggcga
     5401 cgtaggtcgg gtccttgccg ccgagctcga gctgcagctt gatgaaccgc gatccgaccg
     5461 tctgcgcgat gcgcgcgccg gtggcgtgcg agccggtgaa gaacacgccg tcgatgcgct
     5521 gctcgagcag cgccgcgccg accgccgcgc caccgacgag cggcacgaac acctcgctcg
     5581 gcacgccgga ctcgtgcagc agccgtgcaa tgttcagccc ggtgagcgtc gcgaactcgg
     5641 agggcttgta gagcaccgcg ttgccagcca gcagcgccgg cacgaacaca ttgccgccga
     5701 cgaagtacgg atagttccag gccgagatgt tggcgatcac gccgagcgga tcgaagctga
     5761 tcagctcgcg cgtgccggca tcgtcggcga cctgctgcgt tgccagcgtc gtctcggcct
     5821 gcgcgaggaa gaaatcgatg cgcaccagca gcccgttgat ctcgctgcgc gactggcgga
     5881 tcggcttgcc gacctcggcg gtcagcgtga ccgcgagcgt ctcgagctcg gccgcgagcg
     5941 cgacgcgaaa tcgttgcagc atctgcagcc gttgcgccat cggctgcgcg ccccaggcgg
     6001 gttgtgcggc gcgcgccgcg cgcgtgcgtt cggccacgat ctccggcgtg gcttcgggca
     6061 cttcggcgat cagcgcgccg gtggcggggt tgtggatctt cagcatcggt tcgctcgcaa
     6121 agagagtgag agtgggctca ggcgtcgcgg gcggcgcgcg cggtggccag gaagtcggcg
     6181 aggatcgggc cgccgtcgag ctgggcctgg tcgagcgact tcagcgccag gaactccggg
     6241 tgccattgca tgccgaacac gtagctcggc ccgcgccagc ggatcgcctc gacgatgccg
     6301 tcgggcacgc ccagcgcctc gacgaccatg tcgcggccca ggtccttgac ggcctggtgg
     6361 tggatcgagt tgaccttggc ctgctgcacg tccgggtaca tctgcgacaa ccgcgtgccg
     6421 cgcaccaggg tcacgtcgtg cagcgcacgc tcgtagaggt cggcgtcgcg gtgcgagatc
     6481 gcctgcgtga cctgggtcgg gatgtcctgg aacagcgtgc cgcccagcgc cacgttgatc
     6541 agctggcagc cgcggcagat gccgatcacc ggcttgccgg cggcgacgaa ggcgttgaac
     6601 aggtcgatct cgtagcggtc gcgcacccga tcgccggccc aggccggctc cagcggcgtc
     6661 tcgccgtagc tctcgggcgc gacgtcggcg ccgccctgca gcaccaggcc gtcgaggtgc
     6721 tcggcatacg acgccaggct catgtcgccg cgctggatca ggccctcgct ctcgatcgcc
     6781 gggatcatca ccgcgagcac gtccttcgcg agcacccagt gcgcaaccga ctgctcgagg
     6841 tagtgcagcg tcttggtgta gacgccgccg agattgagca ccggcccgac cgggtgatgg
     6901 atgcgcgccg agatgccgat gatcagaggc ctggccatca gaacgcctca tcgaagaagc
     6961 gcgcgaaatc ggcggcctcg cacgggcgcg ggttggtctg gtggcagatg tccgcggtcg
     7021 ccacctcgac cagccgcgga aaatgctcgc gcttgacgcc cacgagcgcc agccccggcg
     7081 ggatgccgat ccggtgcttc agctgcgcga gccagggcac gaagttgctg accgagggca
     7141 cgccgacggc gtgggcgagt tcggcgaact tgtgcggcac ggcctcgcgg ttgtagctca
     7201 tcaccggctc gatcatcagc gcgttcgcca ggccatggtg caggtcgcag accgtgccca
     7261 gcgcgtgcgc gcaggagtgc accgcgccca ggtccttctg gaacgcgatc gcacccatca
     7321 tcgagctcat catcatgtcg gcgcgcgccg ccaggtcggc accgtcgcgc accgcggtga
     7381 ggagcgagcg cgcggcgatc cgcacgcctt cgagcgcaat gccgtcgcac agcgggtgat
     7441 aggccggcga caggtagctc tcgatgttgt gggtcagcgc gtccatcccg gtggcggccg
     7501 tcaccgcggg cggcaggccc agcgtcagct cggggtcggc gaacacgacc ttggcgagga
     7561 tcgccggcga gaagatcacg cgcttcacat gcgtcgcttc ctcgctgatc acgctgctgc
     7621 gcccgacctc gctgccggtg cccgcggtgg tgggcagcgc gatgaagtac ggcagcgcgg
     7681 cggcgtcgat cgcacgcacc tgggggtggt cccacgcgta ctccatcacg tcgccgggat
     7741 gggtggcgag cagcccgacg accttcgcga catcgagtgc ggcgccgccg ccgacgccga
     7801 ccaccgcgtc cgcctcgtgc gccttgtacg cggccgcgcc gtccatcacc tgcgggcctg
     7861 tcgggttgcc gaacacgccg gcgtagaccg cgggcttcag cccggcagct tcgagggcgg
     7921 ccttcagctc ggcgatcagc ggcagcgccg cgaggccctt gtcggtgacg atcagcggcc
     7981 gcttgcagcc gcgctcgttc aggtgcggtc cggcgagcgc actcgcgccg gggccgaagt
     8041 ggatcgtggt cggaaaggaa aaacgggtga tggccatggc gggtgttggt cgtgaacgag
     8101 cggcgggcgc cggtcagatg atctcgaagt agcgcttcag ttcccagtcg gtcacgccat
     8161 ccagccactg gcgccattcc cactcgcggg tcgcggcgaa gtggtcgacg aagtcgtcgc
     8221 cgaaccagtc gcgcgcgacc gccgactgct tgaagacccg cgtcgtctcg atcagggtgc
     8281 gtggcgcgcg cgggatgtgc tctgcccctt cgttggtgcc ggtgatcggc ggcgcgctca
     8341 gcttcagccc ctgctcgacg ccgtgcaggc cggccgcgat caccgcggcc atcgccaggt
     8401 acgggttcac gtcggcgccc gggcagcgcg tctccaggcg ggtggccttc gggctgccgg
     8461 cgatgacgcg aaagctcgcg gtgcggttgt ccatgcccca ggtcggcttc accggcgccc
     8521 agaagccgtc gaccaggcgc ttgtagctgt tgatcgtggg ccagaacatc ggcgccatct
     8581 ccatcaggaa gccgacctgg ccagccaggt agctctcgaa cagcttgctc atccggcgcg
     8641 gcgatttcgc gtcgaagaac aggttgttct tgccgtccga caggctctgg tggatgtgcc
     8701 cgctgcagcc cgggtactgc tggctccact tcgccatgaa gctgggcatc acgccgaagc
     8761 gcgcgccgat ctccttggcg ccggtcttga acaggatcgc gcggtccgcc tgctcgagcg
     8821 ccgcgccgaa cgcgatcgcg acctcgtaga cgcccggccc ggtctcggtg tgcaagcctt
     8881 cgatcggcac gccgaacaag ggcatctcgt ccatcagcgc cttgaagaac tcgcgcttgt
     8941 ggttcatgcg cagcagcgag tagccgaaca tgccgggcgt gatcggctcg ggcgcgatgc
     9001 cgttcttggc ggcccagctc tgtggcgtct cggcgaagtt gaaccactcg aactccatgc
     9061 cggtcagcgc ctgcacgccg agcttctcgg cgcgcttcag aacacgcttg agcgtctggc
     9121 gcgggcagat cggcgacggc gcgccgtcgg cggtgacgaa gtcgccgagg aagaacggca
     9181 cgttgtcgtc ccagggcacg gtgcggtagg tgtcgagatc gagccgcgcg agtgcatcgg
     9241 ggtagccgtg ctgccagccg gtcagcttgg tgttgtcgta gcagttgtcg ccggcgtccc
     9301 agccgaacac cacgctgcag aagccgaagc cgccattggg ctgtggctgc gcggcgccga
     9361 gaaacttgtc gatgtgcagg tatttgccgc gcaggatgcc gtcgatgtcg ctgaccgcga
     9421 ccttgacctt gcaggtgccg ctggcgcgca ccgccttcag cgcggggtgt t
//
LOCUS       DATMJB010000524         3041 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_145293, whole genome shotgun sequence.
ACCESSION   DATMJB010000524 DATMJB010000000
VERSION     DATMJB010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145293"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VNU48_11310"
     CDS             <1..599
                     /locus_tag="VNU48_11310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI11908.1"
                     /translation="PRGKRALAACQRAVAKAVTLTRQLMTFGTAQPGLLLVVDLRRWL
                     PALNEMLRGALRETIAVTIEVDPATSPVQIDPVQFELAILNLALNARDAMNGHGVLRI
                     SAANRRLGRDDVAGLPAGEFVAITVADNGSGIPPEVLGRVFEPFFTTKPVGKGTGLGL
                     SLSYGIVRKHHGQLEVRSESGVGTTFRVTVPVQGSAAP"
     gene            596..1930
                     /locus_tag="VNU48_11315"
     CDS             596..1930
                     /locus_tag="VNU48_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWI11909.1"
                     /translation="MSGALTLPAADEARLDATPPRTILCVDDEPNILSAVRRALRGSG
                     HRVLTAESGQAALQVLASEAVQLVISDMRMPVMDGAQLLEQVRQRWPEVTRILLTGQS
                     DVASTMAAINRGEIFRYISKPWNEDELLMAVHEGLDRHALLHEKVRLERVVALHNEEL
                     TRLNAGLEQQVLDRTAQLSSANARLGKNYLGSIKAFSNLIELRGGSTGGHSRRVAELA
                     RRIAVQMKLPATDVQDVFIASLLHDIGHVGLTDTLLACPVPRMTAEDKTQYEMHPALG
                     EQALMALEGMQPVAMIVRAHHERHDGMGFPDGLRGAAIPIGARFLAVADTYDDLQSGR
                     LARAELAADDARMLVLRGRDTQFDPSVVDAFQELMRARELPPSFVELPVDQLEPGMVL
                     ARDLRSREGVVLLAVDHALSADLIQRIRAHAARHDLSLLIAVQTGRTATTRP"
     gene            2010..2675
                     /locus_tag="VNU48_11320"
     CDS             2010..2675
                     /locus_tag="VNU48_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI11910.1"
                     /translation="MSPTEVPADSDDFTTLEVAKLLGMSVRSVQLMVDRGELDAWKTS
                     GGHRRIARASVDHWRGARGVARPREREPHPQAPAAGRHSAQPVRPRVLLIDDSIHFQK
                     LVALLIQQRFPEIELHVADDGIAGLALYGQLQPQVLIVDLLLPGIDGATLITSLRSHA
                     QFADSRLAVATSLDEQQRAPYAFALEQVPVIHKPRLVAELSDLLATWLPARGAQDATA
                     AAT"
     gene            2685..3005
                     /locus_tag="VNU48_11325"
     CDS             2685..3005
                     /locus_tag="VNU48_11325"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HWI11911.1"
                     /translation="MAAAGVRATIAANFDGDPALYRAFSAACAVQFARDAVAGRAGCD
                     AGDLPAVRRLAHNLKSALLMLGHDELSAMAANIETLAAAGDLVRTRTSWHLLDAALRP
                     LRTP"
BASE COUNT          460 a         1056 c         1059 g          466 t
ORIGIN      
        1 atccgcgcgg caagcgcgcg ctggccgcct gccagcgcgc cgtcgccaag gccgtcacgc
       61 tgacgcgcca gctgatgacc ttcggaacgg cgcagcccgg cctgctgctg gtcgtcgacc
      121 tgcggcgctg gctgccggcg ctgaacgaga tgctgcgcgg cgccctgcgc gaaacgatcg
      181 cggtgacgat cgaggtcgac ccggccacct caccggtgca gatcgacccg gtgcagttcg
      241 agctggccat cctcaacctc gcgctgaacg cgcgtgacgc gatgaacggc catggcgtgc
      301 tgcgcatctc ggccgccaac cggcgcctcg gccgcgacga cgtggccggg ctgccggcgg
      361 gcgagttcgt cgcgatcacg gtcgccgaca acggcagcgg catcccgccc gaggtactgg
      421 gccgcgtgtt cgagcccttc ttcacgacca agccggtcgg caagggcacg gggctggggc
      481 tgtcgctgtc gtacggcatc gtgcgcaagc accacggcca actcgaggtt cgcagcgagt
      541 ccggcgtcgg caccacgttc cgggtcacgg tgccggtgca gggatcggcg gccccatgag
      601 cggggcgctc acgctgccgg cggccgacga ggcgcgcctc gacgcgacgc ccccgcggac
      661 catcctgtgc gtcgacgacg aacccaacat cctctcggcg gtgcgccggg cgctgcgcgg
      721 cagcggccac cgcgtgctga cggccgagag cgggcaggcc gccctgcagg tgctcgccag
      781 cgaagcggtg cagctcgtca tctccgacat gcgcatgccg gtgatggatg gagcgcagct
      841 gctggagcag gtgcgccagc gctggccaga ggtcacacgc attctgctga ccggccaatc
      901 cgacgtcgcc tcgacgatgg cagcgatcaa tcgcggtgag atcttcaggt acatcagcaa
      961 gccgtggaac gaggacgagc tgttgatggc ggtgcacgag gggctcgatc ggcacgccct
     1021 gctgcacgag aaggttcgac tcgagcgcgt ggtcgcgctg cacaacgagg agctcacgcg
     1081 gctcaatgcc ggcctggaac agcaggtgct cgatcgcacg gcacaactgt ccagcgccaa
     1141 cgcgcgactc ggcaagaact acctgggctc catcaaggcg ttctcgaacc tgatcgagtt
     1201 gcgcggcggc tcgacgggcg ggcactcgcg acgcgtggcc gagctggcgc ggcgcatcgc
     1261 cgtgcagatg aagctgcccg cgacggatgt ccaggacgtc ttcatcgcga gcctgctgca
     1321 cgacatcggc catgtcggcc tgaccgacac cctgctcgcc tgcccggtgc cgcgcatgac
     1381 ggccgaggac aagacccagt acgaaatgca cccggcgctc ggcgagcagg cactgatggc
     1441 gctggagggc atgcagccgg tagcgatgat cgtgcgcgcg caccacgaac gccacgacgg
     1501 catggggttc cccgatggcc tgcgcggcgc ggcgatcccg atcggggcgc gctttctggc
     1561 agtggcggac acctatgacg acctgcaatc gggtcgcctc gcgcgtgccg agctggccgc
     1621 cgacgacgcg cgcatgctcg tcctgcgtgg gcgcgacacg caattcgacc cgtccgtcgt
     1681 ggacgcgttc caggagttga tgcgcgcgcg cgaactgccg ccgtcgttcg tcgagttgcc
     1741 ggtagaccag ttggagcccg gcatggtgct tgcacgcgat ctgcgctcgc gcgagggcgt
     1801 ggtgcttctg gcggtggacc acgcgctgag cgccgacctg atccagcgca tccgcgcaca
     1861 cgccgcccga cacgacctgt cgttgctcat cgccgtgcag accggccgca cggcgacgac
     1921 gcgcccctga gagcagcttt tcgtcagcag tcttgccatt ttgtacactt gcacacattt
     1981 ggacactcgc agttttgcag gattgagcca tgagcccgac cgaagtccca gctgattccg
     2041 atgacttcac gacgctggag gtcgcgaagc tcttggggat gtcggtccgc tcggtgcagc
     2101 tgatggtcga tcgcggcgaa ctcgatgcct ggaagacgag cggcgggcac cgccggatcg
     2161 cgcgggcgtc ggtcgaccac tggcgcggcg cgcgcggagt cgccaggccg cgcgagcgtg
     2221 agccgcaccc gcaggcgccg gcggcgggtc gccacagcgc ccagcctgtg cgacctcgcg
     2281 tgctgctgat cgacgattcc attcacttcc agaagttggt ggcgctgctg atccagcagc
     2341 gctttcccga gatcgaactg catgtcgcag acgacggcat cgcggggctt gcgctgtacg
     2401 ggcagctcca gccgcaggtg ctgatcgtgg acctcctgct gccgggcatc gacggtgcca
     2461 cgctgatcac gagcctgcgc tcgcatgcgc agtttgccga cagccgcctc gccgtcgcga
     2521 cctcgctcga cgaacagcag cgtgcgccct atgcgttcgc gctcgagcag gtgcccgtca
     2581 ttcacaagcc tcggctggtc gcggaacttt cggacctgct ggcgacctgg ctgcccgcgc
     2641 gcggggcgca agacgccacg gccgcagcga cctgagcgct ggccatggct gcggcaggcg
     2701 tgcgtgccac catcgccgcc aatttcgatg gcgatccggc gctgtaccgg gccttctcgg
     2761 cggcgtgtgc ggtgcagttc gcgcgcgatg ccgttgccgg tcgagccggc tgcgatgcgg
     2821 gcgatctgcc cgcagttcgc aggctcgcgc acaacctcaa gtcggcgctg ctcatgctcg
     2881 gccatgacga actgagcgcg atggcggcga acatcgaaac gctggcggcg gcaggcgatc
     2941 tggtgcgcac gcgcacgtcc tggcatttgc tggatgccgc gctgcggccg ttgcgcacgc
     3001 catgagcccc gcccggccgc ccgaaggggc tcattcccgc t
//
LOCUS       DATMJB010000525         3050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_802711, whole genome shotgun sequence.
ACCESSION   DATMJB010000525 DATMJB010000000
VERSION     DATMJB010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802711"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VNU48_11330"
     CDS             <1..441
                     /locus_tag="VNU48_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HWI11912.1"
                     /translation="KPPKYSRAVMETLAIIAYRQPVTRGDIEDIRGVTVASQIVKQLE
                     DRGWVEAIGYRESPGRPALLATTKQFLDDLGLASLEQLPLLAGATPDAEQFAHVLPEQ
                     ASLLDAPADAAPASTEAATEPATSPTPDLPDEPAAHAAPMEPQA"
     gene            438..2420
                     /locus_tag="VNU48_11335"
     CDS             438..2420
                     /locus_tag="VNU48_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWI11913.1"
                     /translation="MNVNPESSLPPADDAPAADAADSPAKPARKRRSVKAAEEVVTTV
                     AHDAGEVAAAEAVPKPKRRRASAAAPVEAGDAPDVVAVPATEHASVPEPARVETIAPD
                     APAQAQSAEAAASPEASEGFGAATNQDGSDGPRSRRRGRRDRKRGDRGEPRDPNNTEP
                     PQDGDAPVLAPQTGELFAQVLSGEFDVNGEPEPVPELSEEEAAAAPKRVLLPEADAPK
                     LHKVLAQSGIGSRRDMEDLIADGHVMVNGEPAHVGQRVSFGDRVQVNGKPVKLRIVPP
                     PPRIIAYHKPVGEIVTHDDPQNRPTVFRRLPRLQQGKWQSVGRLDINTEGLLLFTNSG
                     ELANQLMHPRFGVEREYAVRLLGTLTEENKTNLLAGVDVEGQKASFKSITEGGGDGAN
                     HWYRVVITEGRNREVRKLFDTVGLTVSRLIRIRYGTVVLPFGLKRGVWVDLEEGDVRA
                     IRRLASGDSNAAREGRGGRGNEARQGGQPQQRNDRPRDNNQGGRQNDRPRGPRRDGNA
                     PMPAPQGLVEGIVGHRSNDSRDDGDEDLDFDPSSIPNPLEQTFDKRFVSNPRSPVGGR
                     GFRNGGGGGFGAGGSGPAPGPKRGGGGQAGGPKQPDPLQTSLGVFGADAFHRKNRGGG
                     GGGGGRGGSRGGGGGGFGGGNSGGGFGGGGGGRRGR"
     gene            2505..2930
                     /gene="ndk"
                     /locus_tag="VNU48_11340"
     CDS             2505..2930
                     /gene="ndk"
                     /locus_tag="VNU48_11340"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWI11914.1"
                     /translation="MAVERTLSIIKPDAVAKNVIGQIYARFEAAGLKIVAAKMAHLSR
                     GEAEAFYAVHKARPFFKDLVDFMISGPVMIQALEGEGAIMKNRDLMGATDPKKAEKGT
                     IRADFAASIDANAVHGSDAAETAATEIAFFFPGMNVYSR"
BASE COUNT          510 a         1063 c         1091 g          386 t
ORIGIN      
        1 aagccgccca agtattcgcg cgcggtgatg gagacgctgg cgatcattgc gtaccgccag
       61 ccggtcacgc gtggcgacat cgaggacatc cgcggtgtca ccgtggcgag ccagatcgtc
      121 aagcagctcg aagaccgcgg ctgggtcgag gcgatcggct accgcgagtc gccgggccgt
      181 ccggcgctgc tggccacgac caagcagttc ctcgacgacc tgggcctggc cagcctcgag
      241 caactgccgt tgctcgcggg cgcgacgccc gacgccgagc agtttgcgca cgtgcttccc
      301 gagcaggcct cgttgctcga cgcgcccgcc gacgccgccc cggcgtccac tgaagcggcc
      361 accgagcccg cgacatcacc cacccccgat ctccccgacg agcctgcggc gcacgccgcg
      421 cccatggaac cccaagcatg aatgtcaacc cagaatcttc gctgccgccg gccgacgatg
      481 cccccgccgc cgatgcggcc gacagccccg cgaagccggc gcgcaagcgc cgcagcgtga
      541 aggcggccga agaggtggtg acgacggtcg cgcacgacgc cggcgaagtg gccgccgccg
      601 aggccgtgcc gaagccgaag cggcgtcgcg ccagcgcggc tgcgcctgtc gaggccggcg
      661 acgcgccgga tgtcgtcgca gtgcccgcga cggagcacgc gagtgtcccc gagcccgcgc
      721 gcgtcgagac gatcgcgccc gacgctccgg cgcaagcgca gtccgccgaa gctgccgcgt
      781 cgcccgaggc ttcggagggc ttcggcgccg cgacgaatca agacggctcc gacggcccgc
      841 gcagccggcg ccgcgggcgc cgcgatcgca agcgcggtga ccgcggcgag ccgcgcgatc
      901 cgaacaacac cgagccgccg caggacggcg atgcgccggt gctcgcaccg cagaccggcg
      961 agttgttcgc gcaggtgctg tcgggcgagt tcgacgtcaa cggcgagccc gagccggtgc
     1021 cggaactttc cgaagaagaa gcggctgccg cgcccaagcg cgtgctgctg cccgaggccg
     1081 acgcgccgaa actgcacaag gtgctggcgc agtccggcat cggttcgcgc cgcgacatgg
     1141 aagacctgat cgccgacggc cacgtgatgg tcaacggcga gccggcgcat gtcggccagc
     1201 gcgtgtcgtt cggcgaccgg gtgcaggtca acggcaagcc ggtcaagctg cgcatcgtgc
     1261 cgccgccgcc gcgcatcatc gcgtaccaca agccggtcgg cgagatcgtc acccacgacg
     1321 atccgcagaa tcggccgacc gtgttccgac gtctgccgcg tctgcagcag ggcaagtggc
     1381 agtcggtcgg ccgcctggac atcaacaccg aagggctgct gctgttcacg aattcgggcg
     1441 aactggccaa ccagctgatg cacccgcgct tcggtgtcga gcgcgagtac gcggtgcgcc
     1501 tgctcggcac gttgaccgaa gagaacaaga ccaacctgct cgccggtgtc gacgtcgagg
     1561 gccagaaggc gagcttcaag tcgatcaccg aaggcggcgg tgacggcgcg aaccactggt
     1621 accgcgtcgt catcaccgag ggccgcaacc gcgaggtgcg caagctgttc gacacggtcg
     1681 ggctgacggt cagccggctg atccgcatcc gctacggcac ggtcgtgctg ccgttcgggc
     1741 tgaagcgcgg tgtctgggtc gatctcgaag aaggcgacgt tcgcgcgatc cggcggctgg
     1801 ccagcggcga cagcaacgct gcgcgcgaag gtcgcggcgg gcgcggcaac gaagcgcgtc
     1861 agggtggcca gccgcagcag cgcaacgacc gcccgcgcga caacaaccag ggtggccgcc
     1921 agaacgatcg cccgcgcggc ccgcggcgcg acggcaatgc gccgatgccg gcgccgcaag
     1981 gcctggtcga aggcatcgtc ggccaccgca gcaacgacag ccgtgacgat ggcgacgagg
     2041 acctggattt cgacccgtcg tcgatcccga atccgctcga gcagaccttc gacaagcgct
     2101 tcgtgtcgaa cccgcgcagc ccggtgggcg ggcgcggatt ccgcaatggt ggcggcggcg
     2161 gtttcggtgc cggcggcagc gggccggcgc cggggccgaa acgtggcggc ggcggtcagg
     2221 ccggtggccc gaagcagccg gatccactgc agacctcgct cggcgtgttc ggggccgatg
     2281 cgttccatcg caagaaccgt ggcggcggcg gcggcggtgg tgggcgcggt ggcagtcgtg
     2341 gcggcggtgg cggcggcttt ggcggcggca acagcggtgg cggtttcggc ggcggcggcg
     2401 gcggccggcg cggacgctga gcgcgcgccg ggttcatcgt cgcgaaagca ccgcacgcta
     2461 caatctaagg ctttgccaag tacttgattt caggagagaa aaccatggcc gtcgaacgca
     2521 cgctttcgat catcaaaccc gacgccgtcg cgaagaacgt gatcgggcag atctacgccc
     2581 gcttcgaggc cgccggcctg aagatcgttg ccgccaagat ggcgcacctg tcgcgcggcg
     2641 aggccgaggc cttctatgcg gtgcacaagg cgcgcccgtt cttcaaggac ctggtcgact
     2701 tcatgatctc cggcccggtg atgatccagg cgctggaagg cgagggcgcg atcatgaaga
     2761 accgtgacct gatgggcgcg accgatccga agaaggccga gaagggcacg atccgcgccg
     2821 acttcgccgc cagcatcgac gccaatgcgg tgcacggctc cgatgccgcc gagaccgcgg
     2881 ccaccgagat cgcgttcttc ttccccggca tgaacgttta cagccgctga agactcgaca
     2941 ggaactggtc atgaacatcg cggagccggg ccgagcgccg ggccgctccc aagccggccc
     3001 ggactccccc ggggggtcga ccgacgtacc cgtcggtcgg ggggctccat
//
LOCUS       DATMJB010000526         2854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_739710, whole genome shotgun sequence.
ACCESSION   DATMJB010000526 DATMJB010000000
VERSION     DATMJB010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739710"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..554
                     /gene="purE"
                     /locus_tag="VNU48_11345"
     CDS             90..554
                     /gene="purE"
                     /locus_tag="VNU48_11345"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HWI11915.1"
                     /translation="MGSSSDWDTLRAATEILAEFGVPHEARVVSAHRMPDEMFAYAQE
                     AAARGLKAIIAGAGGAAHLPGMLAAKTTVPVLGVPVASRHLQGVDSLHSIVQMPRGIP
                     VATFAIGAAGAANAALFAVAMLANDDPALRAKLDAFRARQTEAARAMTADLK"
     gene            659..1813
                     /locus_tag="VNU48_11350"
     CDS             659..1813
                     /locus_tag="VNU48_11350"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HWI11916.1"
                     /translation="MAPGATLGVMGGGQLARMFVHAAQQMGYATAVLDADPASPAGLI
                     AHHHIRSDYLDPQGLTQLADLSAAVTTEFENVPARALALLAQRRPVAPGAAAVAVCQD
                     RAAEKAHFERSAVACAPHAVIESAGQLAAGRDELLPGILKTARLGYDGKGQRRVATRA
                     GLASAWAALGGVPCVLEKMLPLAHEISVIVARGADGRIVHLPVQQNLHRDGVLALTQV
                     PAPDVPAALQQQALDSASRIAAELGYVGVLCVEFFVLQDGTLVANEMAPRPHNSGHYS
                     IDACDVSQFELQVRAMTGAPLLAPRLHSPCVMLNLLGDLWFDAHGGLRAGGPDWAAVL
                     ALPGAHLHLYGKAQPRAGRKMGHLTITAASAAQADAVAREAVARLGLEGW"
     gene            1816..2796
                     /locus_tag="VNU48_11355"
     CDS             1816..2796
                     /locus_tag="VNU48_11355"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWI11917.1"
                     /translation="MRLAGSDAAAVEQAAERLAASELVAFPTETVYGLGARADDAGAV
                     AKIFAAKGRPADHPLIVHVAGPLQIARFAAEVPTLAQRLIDAFWPGPLTVILPRLPAI
                     AAAAAGGQASIGLRCPSHPVAHALLAAAQRRGVPGVAAPSANRFGRVSPTTALHVIDE
                     FGDALTVLDGGACEVGIESSIVDCSRGRPVLLRPGVLTRARIEAAAGAPLLEPDADAP
                     RAPGTLAAHYAPRARVRLMPAPLLHTALQVLGDEAATLAVYSRGVLPAAARGIRHRRM
                     PALPAQAAHELFSVLRELDAEGVQLIWVEEPPPEPEWDGVRDRLARAAAA"
BASE COUNT          351 a         1069 c         1044 g          390 t
ORIGIN      
        1 ggagatggcg ccgtgccgcc tacaattcaa ggtttcacca gttcctggag cgagctttga
       61 gcagcgccgc tgtggtggtg ggcgtggtga tgggttccag cagcgactgg gacactctgc
      121 gcgccgccac cgagattctc gccgagttcg gcgtccccca cgaagcccgc gtcgtctcgg
      181 cccaccgcat gccggacgag atgttcgcgt atgcgcagga agccgcggcg cgcggcctga
      241 aggcgatcat cgccggcgcc ggcggcgcgg cgcacctgcc cggcatgctg gccgcgaaga
      301 cgacggtgcc ggtgctcggc gtgccggtcg cgagccggca tctgcagggt gtcgattcgc
      361 tgcattcgat cgtgcagatg ccgcgcggca tcccggtcgc gaccttcgcg atcggcgccg
      421 ccggcgcggc caatgcggcg ctgttcgcgg tcgcgatgct ggccaacgac gacccggcgc
      481 tgcgcgcgaa actcgacgcg tttcgcgccc gccagaccga ggccgcacgc gcgatgacgg
      541 ccgatctcaa gtgaccccgt tcgtggcgcc cggcgccacg ctcggtgatg ggccacaagc
      601 gaccccgttc gtggcgcctg gcgccacgct cggtgatggg ccacaagcga ccccgttcgt
      661 ggcgcccggc gccacgctcg gtgtcatggg cggcggccag ctcgcgcgca tgttcgtgca
      721 cgccgcgcag cagatgggct acgcgaccgc cgtgctcgat gccgatccgg caagtccggc
      781 cgggctgatc gcgcaccatc acatccgcag cgactacctc gatccgcaag ggctgacgca
      841 gctcgccgac ctgagcgctg cggtgacgac cgaattcgag aacgtgccgg cgcgcgcgct
      901 cgcgctgctg gcgcagcgcc ggccggtcgc acccggcgcc gcggcggtcg cggtctgcca
      961 ggaccgcgcc gccgagaagg cgcacttcga gcgcagcgcg gtggcctgtg cgccgcacgc
     1021 ggtgatcgaa tcggccggcc agctcgcggc gggtcgtgac gaactgctgc ccggcatcct
     1081 gaagaccgcg cgcctgggct acgacggcaa gggccagcgg cgggtcgcga cccgggccgg
     1141 gctcgcgtcc gcctgggctg cgctcggcgg cgtcccctgc gtcctcgaga agatgctgcc
     1201 actggcgcac gagatcagcg tgatcgtcgc gcgcggcgcc gacggccgga tcgtccacct
     1261 gccggtgcag cagaacctgc accgcgacgg cgtgctcgcg ctcacgcagg tgccggcgcc
     1321 cgacgtgccc gccgcgctgc agcagcaggc gctcgacagc gccagccgca tcgctgccga
     1381 gctcggctac gtgggcgtgc tgtgcgtcga gttcttcgtc ctgcaagacg gcacgctggt
     1441 cgccaacgag atggcgccgc gcccgcacaa ctcgggccac tacagcatcg acgcctgcga
     1501 tgtctcgcag ttcgagctgc aggtccgggc catgaccggt gcaccgctgc tcgcgccgcg
     1561 cctgcactcg ccgtgcgtga tgctgaacct gctcggcgac ctgtggttcg acgcgcacgg
     1621 cggcctgcgc gccggcggcc ccgactgggc cgcggtgctc gcgctgccgg gcgcgcacct
     1681 gcatctgtac ggcaaggcgc agccgcgcgc cggtcgcaag atggggcacc tgaccatcac
     1741 cgccgcgagc gcggcgcagg ccgacgcggt ggcgcgcgag gccgtggccc gcctcggcct
     1801 cgaaggctgg tagcgatgcg gctggccggg tcggacgctg cggccgtcga acaggcggcc
     1861 gagcggctcg ccgccagcga actcgtggcc ttcccgaccg agaccgtcta cgggctgggc
     1921 gcgcgcgccg acgacgccgg cgcggtcgcg aagatcttcg ccgccaaggg ccggccggcg
     1981 gaccatccgc tgatcgtgca cgtggccggg cctttgcaga tcgcgcggtt cgccgccgag
     2041 gtgccgacgc tggcgcagcg cctgatcgac gcgttctggc ccgggccgct gaccgtgatc
     2101 ctgccgcgcc tgccggccat cgccgcggcc gcggccggcg gccaggccag catcggcctg
     2161 cgctgcccgt cgcacccggt cgcacacgcc ctgctggcgg cggcgcagcg gcgcggcgtg
     2221 cccggcgtgg ccgcgccgag cgcgaaccgc ttcggccgcg tcagcccgac caccgcactc
     2281 cacgtgatcg acgagttcgg cgacgcgctc acggtgctcg acggcggtgc ctgcgaggtc
     2341 ggcatcgaat cgagcatcgt cgactgctcg cgcggccgcc cggtgctgct gcgccccggc
     2401 gtgctgacgc gcgcgcgcat cgaggcggcg gccggcgcgc ccttgctgga acccgatgcg
     2461 gacgcgcccc gcgcgcccgg cacgctggcg gcgcactacg ccccgcgcgc ccgggtgcgc
     2521 ctgatgccgg cgccgctgct tcacacggct ttgcaagtgc tgggcgatga agcggcgacg
     2581 ctggcggtat attcccgcgg cgtactgcct gcggccgccc ggggcattcg gcaccggcgc
     2641 atgccggcgc tgcccgccca ggcggcgcac gagttgttct cggtgttgcg cgaactcgat
     2701 gccgagggcg tgcaactgat ctgggtcgag gagccaccgc ccgagcccga atgggacggg
     2761 gtgcgcgacc gactggcgcg cgccgccgcc gcctgacccg tctttccccc catgctgctg
     2821 accgccgctg atttttcgga gccctgaatg aagt
//
LOCUS       DATMJB010000527         4858 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_853210, whole genome shotgun sequence.
ACCESSION   DATMJB010000527 DATMJB010000000
VERSION     DATMJB010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853210"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="VNU48_11360"
     CDS             complement(<1..712)
                     /locus_tag="VNU48_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI11918.1"
                     /translation="MQPPARNATRSAHPPAGIERELWLLHLEDSELDHELTLAHLARG
                     GLRARMRRIDSEAEFLDALEEHDWDAIISDYNLPGFSGLVALDLLKASGRDLPFILVS
                     GEIGEDTAVEAMRNGASDYLLKNNLSRLVPALLHAVEATETRRARERADRELDASKQR
                     LRELAQHLQNSIEQERTAIAREIHDDVGGSLTALKFDLAWIARHSDSDEVLTRVNSAL
                     ETVTLAIDSSKRIMHNLRP"
     gene            854..1312
                     /locus_tag="VNU48_11365"
     CDS             854..1312
                     /locus_tag="VNU48_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11919.1"
                     /translation="MPNTHAPEPNTTAPMPLLVAADLQDHLMTATHDLDRLQALLTHA
                     CDALMLGFHGATQRLHAIAEASAGGARTLDLASDAARQLGGAVTALQFQDMASQLIAH
                     THQRLRSCADRLARETMGDDDDGAALVEAAPLRPNPVTQDEMDAGSIELF"
     gene            1376..1738
                     /locus_tag="VNU48_11370"
     CDS             1376..1738
                     /locus_tag="VNU48_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI11920.1"
                     /translation="MHSILAVDDSASMRQMVAFTLKSAGYSVVEAVDGQDAWEKTGKS
                     DFDLVLTDQNMPRLDGIGLTRKLRGNPKFKATPILILTTESSDQMKQAGRDAGATGWL
                     VKPFDPAKLIEVIKKVIR"
     gene            1773..3911
                     /locus_tag="VNU48_11375"
     CDS             1773..3911
                     /locus_tag="VNU48_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HWI11921.1"
                     /translation="MGDMTTEGANIGAGIDLSQFYQVFFEEAGENLDRMEAQLLEIDI
                     EAADDEELNSIFRCAHSVKGGAATFGFADVAELTHQMETLLDKLRRHELTPTAAMVDV
                     LLQAGDALRAQLARHQGAGGDAVDTAELLASIRAMTTGKAPAAPAVPEPAVPAAPVRE
                     ELPLDAAPAPAEGQAREVELRVGPLADPARADNLVELFKEITDLGTIEAIDGGQASDG
                     MRRFRIITTSSDADLLDLFTFHVAREEVALLPMTPGYGFHAGAPGAPPESELAQHDPG
                     YGFFDNAPGAPGTEAPVAGVVAEAEAAADVAKPAARAPARVEKAPAAALESSTLRVSV
                     EKVDQLINLVGELVITQAMLAQNSKQIDTALYQQLVGGLADLERNTRDLQESVMSIRM
                     IPMSMVFSRFPRMLRDLAAKLGKKVEFVTQGEATELDKGLVEKITDPLTHLVRNSCDH
                     GIESPAERLAKGKPEHGTITLAASHQGGSILIEVRDDGKGLSRDKLIAKARERGIDAP
                     DSMSDAEVWSLIFAPGFSTAEVVTDVSGRGVGMDVVKKNITALGGTVEIDSAEGYGMS
                     VKVRLPLTLAIMDGMSVGVGDEVYILPLSSVIESFQVQPDTIKTIGGSGRVIEVRDEF
                     MPVLDLERIFEVPRVDFDSASGIMVVAEAEGGRVALLVDQLLGQQQVVVKNLEANYRR
                     VDDISGATIMGDGRVALILDVGSLVRRSRH"
     gene            4123..4587
                     /locus_tag="VNU48_11380"
     CDS             4123..4587
                     /locus_tag="VNU48_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HWI11922.1"
                     /translation="MNKQLLIAGLAASFGVAGLTNAFAAEGGATAAEATAMVKKGVAF
                     IKASGTDKGYAAISTKGGQFSDRDLYLVVYGLDGTVRAHGANEKMIGKNLIDLKDVDG
                     KAFVKERVELAASKGTFWQDYKFTNPENKKIEPKSMYCEKLDDAVVCGGIYK"
     gene            4654..>4858
                     /locus_tag="VNU48_11385"
     CDS             4654..>4858
                     /locus_tag="VNU48_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11923.1"
                     /translation="MLAPALTGAMLALTLAASIWVLNAYQTRSEGAHRQVMEAYAKIT
                     TAQSKLGELHTQLYRTVTIANSLD"
BASE COUNT          845 a         1707 c         1642 g          664 t
ORIGIN      
        1 ccgggcgcag gttgtgcatg atgcgcttgc tcgagtcgat cgccagcgtc acggtctcga
       61 gcgccgagtt gacgcgcgtc agcacctcgt cggagtcgga atggcgggcg atccacgcca
      121 gatcgaactt cagcgccgtc agcgagccgc cgacatcgtc gtggatctcg cgcgcgatcg
      181 cggtgcgttc ctgttcgatg ctgttctgca ggtgctgggc gagttcgcgc aggcgctgct
      241 tcgacgcgtc gagctcgcgg tcggcgcgct cgcgcgcgcg ccgcgtctcg gtcgcctcga
      301 cggcgtgcag cagcgccggc acgagccggc tcaggttgtt cttcagcagg tagtcgctcg
      361 cgccgttgcg catcgcctcg accgcggtgt cctcgccgat ctcgcccgag actaggatga
      421 acggcaggtc gcggccgctg gccttcagca ggtcgagcgc gaccaggccc gagaacccgg
      481 gcaggttgta gtcggagatg atcgcgtccc agtcgtgctc ttcgagcgcg tcgaggaact
      541 cggcctcgga atcgatgcgc cgcatccgcg cgcgcagccc gccgcgggcc aggtgcgcca
      601 gcgtcagttc gtgatccagt tccgaatctt ccaggtgcag cagccacagc tcgcgctcga
      661 tgcccgccgg cgggtgggcc gatcgggtcg cgttcctggc tggtggctgc atggcgtcga
      721 gtatgccccg cggcgtcgcg gccgggcaac cggggccggg cgccggccac tcggcctcag
      781 gtccgccggc cgattgccga aaagacggca tgcgccaacc cggcaccacc gggccgactc
      841 cgaaacacgc acgatgccga acacccacgc ccccgagccg aacacgactg cgccgatgcc
      901 gctgctcgtc gccgccgacc ttcaggacca cctgatgacg gccacgcacg acctcgaccg
      961 cctgcaggcg ctgctcacgc acgcctgcga cgcgctgatg ctgggctttc acggcgcgac
     1021 gcagcggctg cacgcgatcg ccgaggcctc cgccggcggc gcgcgcacgc tggacctggc
     1081 cagcgacgcg gcgcggcagc tgggcggcgc ggtcaccgcg ctgcagttcc aggacatggc
     1141 ctcgcaactg atcgcccaca cgcaccagcg cctgcgcagc tgcgccgacc gcctggcgcg
     1201 cgaaaccatg ggcgacgacg acgacggcgc ggcgctggtc gaagccgcac cgctgcggcc
     1261 gaatccggtc acgcaggacg agatggacgc gggctccatc gagctgttct agcgcgtcgc
     1321 gtctcaagaa actgccccga acaaccgaca acctcccagg aatccggaga ctcccatgca
     1381 ttcaatcctc gccgtcgacg actcggcgtc catgcgccag atggtcgcgt tcacgctgaa
     1441 aagcgcaggc tacagcgtcg tcgaggccgt cgacggccag gacgcctggg aaaagaccgg
     1501 caagagcgac ttcgacctgg tgctgaccga ccagaacatg ccgcgcctgg acggcatcgg
     1561 cctgacccgc aagctgcgcg gcaaccccaa gttcaaggcc acgccgatcc tgatcctgac
     1621 gaccgagtcg agcgaccaga tgaagcaggc cggccgcgac gccggcgcca cgggctggct
     1681 ggtcaagccc ttcgatccgg ccaagctgat cgaagtgatc aagaaggtga tccgctgaac
     1741 gcggacggac gactgcacgc gaggagcatt gcatgggtga catgaccaca gagggtgcga
     1801 acatcggcgc gggcatcgac ctgagccagt tctaccaggt gttcttcgag gaagcgggcg
     1861 agaacctcga ccgcatggag gcgcagctgc tcgagatcga catcgaggcc gccgacgacg
     1921 aggagctgaa ctcgatcttc cgctgcgcgc attcggtcaa gggcggcgcc gcgaccttcg
     1981 gcttcgccga cgtcgccgag ctgacgcacc agatggagac gctgctcgac aagctgcgcc
     2041 gccacgaact gacgccgacc gcggcgatgg tcgacgtgct gctgcaggcc ggcgacgcgc
     2101 tgcgcgcgca gctggcgcgc caccagggcg cgggcggcga cgcggtcgac accgccgagc
     2161 tgctggcgag catccgcgcg atgacgaccg gcaaggcgcc ggccgcgccg gcggtgccgg
     2221 aacccgccgt accggccgcg ccggttcgcg aggaactgcc gctggatgcg gccccggcgc
     2281 cggccgaagg ccaggcgcgt gaggtcgagc tgcgcgtcgg gccgctcgcc gacccggcgc
     2341 gggccgacaa cctggtcgag ctgttcaagg agatcaccga cctgggcacg atcgaggcga
     2401 tcgacggcgg ccaggccagc gacggcatgc ggcgcttccg catcatcacg accagcagcg
     2461 acgccgacct gctggacctg ttcaccttcc acgtcgcgcg cgaggaagtg gcgctgctgc
     2521 cgatgacccc cggctacggc ttccatgccg gtgcgccggg cgcgcccccg gaatccgaac
     2581 tcgcccagca cgaccccggc tacggcttct tcgacaacgc accgggcgcg ccgggcaccg
     2641 aagcccccgt cgcgggcgtc gtggccgagg ccgaggccgc ggccgacgtc gccaagcccg
     2701 cggcgcgcgc cccggcccgc gtcgagaagg cgccggccgc cgcgctcgaa tcgtcgaccc
     2761 tgcgcgtctc ggtcgagaag gtcgaccagc tgatcaacct ggtcggcgaa ctcgtcatca
     2821 cgcaggcgat gctggcgcag aacagcaagc agatcgacac cgcgctgtac cagcagctgg
     2881 tcggcgggct ggccgacctg gaacgcaaca cccgcgacct gcaggaatcg gtgatgtcga
     2941 tccggatgat tccgatgtcg atggtgttct cgcgcttccc gcgcatgctg cgcgacctgg
     3001 cggccaagct cggcaagaag gtcgagttcg tcacgcaggg cgaggccacc gagctcgaca
     3061 agggcctggt cgagaagatc accgacccgc tgacgcacct ggtgcgcaac agctgcgacc
     3121 acggcatcga atcacccgcc gagcgcctgg ccaagggcaa gcccgaacac ggcacgatca
     3181 cgctcgccgc ctcgcaccag ggcggctcga tcctgatcga ggtgcgcgac gacggcaagg
     3241 gcctgtcgcg cgacaagctg atcgcgaagg cgcgcgaacg cggcatcgac gcgccggatt
     3301 cgatgagcga cgccgaggtc tggagcctga tcttcgcgcc cggcttctcg accgccgagg
     3361 tcgtcaccga cgtgtcgggc cgcggcgtcg gcatggacgt ggtgaagaag aacatcaccg
     3421 cgctcggcgg caccgtcgag atcgactcgg ccgagggcta cggcatgagc gtcaaggtgc
     3481 gcctgccgct gacgctggcg atcatggacg gcatgtcggt gggcgtcggc gacgaggtct
     3541 acatcctgcc gctgtcgtcg gtgatcgaat cgttccaggt ccagccggac acgatcaaga
     3601 cgatcggcgg cagcggccgc gtcatcgagg tgcgcgacga gttcatgccg gtgctcgacc
     3661 tggaacggat cttcgaggtg ccgcgcgtcg atttcgacag cgcgagcggc atcatggtcg
     3721 tcgccgaagc cgagggcggg cgcgtcgcac tgctggtcga ccagttgctc ggccagcaac
     3781 aggtggtcgt caagaacctc gaggcgaact accggcgtgt cgacgacatc tcgggcgcga
     3841 cgatcatggg cgacggccgc gtcgcgctga tcctcgacgt cggcagcctg gtgcggcgct
     3901 cgcgccactg aacatcgcgc agcccgtcac actggccggc cccacgacag tgcggccggc
     3961 cttctcgctt ctgcggcagc gctcaatcgg gcccgccggc ggccgataac aagcctgacc
     4021 cctgaattca tcttcccgct ccgttccgca tgactccgag caatctgcgc cgggccgcgg
     4081 ccctctccaa cccctgactc accccaccga aaggaactca gcatgaacaa gcaactcctc
     4141 atcgccggcc tggcggcctc cttcggcgtc gccggcctca ccaacgcctt cgccgcggaa
     4201 ggcggcgcca ccgccgcgga ggccaccgcg atggtcaaga aaggcgtggc cttcatcaag
     4261 gccagcggca cggacaaggg ctatgccgcg atcagcacca agggtggcca gttctccgac
     4321 cgcgacctgt acctggtcgt ctacggcctc gacggcacgg tgcgcgcgca tggcgcgaac
     4381 gagaagatga tcggcaagaa cctgatcgac ctgaaggacg tggacggcaa ggccttcgtc
     4441 aaggagcgtg tcgaactggc ggcgagcaag ggcacgttct ggcaggacta caagttcacc
     4501 aacccggaga acaagaagat cgagccgaag agcatgtact gcgagaagct cgacgacgcc
     4561 gtggtctgcg gcggcatcta caaataagcc gggcggccgg ccctctcgag atctccaacg
     4621 gacacacgca cgatgaaact tcgcaacaaa ctgatgctgg ctccggccct gacgggtgcg
     4681 atgctcgcgc tgacactggc ggcctcgatc tgggtgctga acgcctacca gacccgcagc
     4741 gagggcgcgc accggcaggt gatggaagcc tacgccaaga tcaccacggc gcagtcgaag
     4801 ctcggcgagc tgcacacgca gttgtaccgg acggtcacga tcgcgaactc gctcgacg
//
LOCUS       DATMJB010000528         2327 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_736136, whole genome shotgun sequence.
ACCESSION   DATMJB010000528 DATMJB010000000
VERSION     DATMJB010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736136"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VNU48_11390"
     CDS             <1..194
                     /locus_tag="VNU48_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HWI11924.1"
                     /translation="MALIPLRAGAAEGSDASAVPAFAMLVLASPDPTRYTADMGTEFL
                     SRIGDIAGAALSRLLPVRS"
     gene            205..1173
                     /locus_tag="VNU48_11395"
     CDS             205..1173
                     /locus_tag="VNU48_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HWI11925.1"
                     /translation="MSEPAQRPNAAPALDDDIRRHLVHLEVERRLAGRTLTLYRAALA
                     KLQAFAAAQSLPLREVQVHHIRRWAAALHGQGLAPRSIALVLSAWRGLYRWLGRDGLV
                     ALNPADGVRAPKAPKPLPKALSVDLAMALADAPRTGDDAAADAPAIAVRDQCIVELLY
                     GCGLRVGELVGLDLRPGGAGWIDVADASAHVLGKGSKRRSVPVGGAALKALNAWLALR
                     GNLARSDEPALLVGSRGARLGASQVRARLKLRALRAGLPTHVHPHMLRHSFATHVLQS
                     SGDLRAVQELLGHANITTTQVYTKLDFGHLSKVYDAAHPRAKKQRG"
     gene            complement(1186..1674)
                     /locus_tag="VNU48_11400"
     CDS             complement(1186..1674)
                     /locus_tag="VNU48_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWI11926.1"
                     /translation="MNDLVTLDSADLRILAQLQQDSSLSNLALAAATHTSAATCLRRV
                     KRLVANGVIERQVAILSPQKMGAGLTAIAEVSLDRQGAEHLAEFEARAVAQPEVQQCY
                     RVSPGPDLVLVLQVFDMAQYHALVQRLFTQDANVRNVKTFFSVHRAKFETRVPLPAAA
                     VG"
     gene            1811..>2327
                     /locus_tag="VNU48_11405"
     CDS             1811..>2327
                     /locus_tag="VNU48_11405"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721392.1"
                     /note="Catalyzes the first step in hexosamine metabolism,
                     converting fructose-6P into glucosamine-6P using glutamine
                     as a nitrogen source; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HWI11927.1"
                     /translation="MCGIVGAVSTRNIVPILIEGLKRLEYRGYDSCGVAVHQNGELRR
                     ARSTARVAELEAEVARDAVQAGTGIAHTRWATHGAPAVHNAHPHFSRGPGVSDAAVAV
                     GGGGAQAGRIALVHNGIIENHDELRAELQARGYVFASQTDTEVIAHLVDHLYDGDLFD
                     AVQRALPRLRGA"
BASE COUNT          334 a          850 c          809 g          334 t
ORIGIN      
        1 cgatggcgct gatcccgctg cgcgcggggg cggccgaggg cagcgacgcg tcggcggtgc
       61 cggcgttcgc catgctcgtg ctggcctcgc ccgacccgac gcgctacacc gccgacatgg
      121 gcaccgagtt cctctcccgc atcggcgaca tcgccggcgc cgcgctgtcg cggttgctgc
      181 cggtgcgcag ctgacgcaac cgccatgagc gaaccggcgc agcgccccaa cgcggcgccc
      241 gcgctcgacg acgacatccg gcgccacctc gtccacctcg aggtcgaacg gcgcctggcg
      301 gggcgcacgc tgacgctgta ccgcgcggcg ctcgcgaagc tgcaggcctt cgccgccgcg
      361 cagtcgctgc cgttgcgcga ggtgcaggtt catcacatcc gccgctgggc ggcggcgctg
      421 catggccagg ggctggcgcc gcgcagcatc gcgctcgtgc tgtcggcctg gcgcggcctg
      481 taccgctggc tgggtcgcga tgggctggtg gcgctgaacc cggccgacgg cgtgcgcgcg
      541 ccgaaggcgc ccaagccgct gccgaaggca ctgtcggtgg acctcgcgat ggcgctggcc
      601 gacgcgccgc gcacgggcga tgacgccgcg gccgacgccc cggcgatcgc ggtgcgcgac
      661 cagtgcatcg tcgagctgct ctacggttgc ggactgcgcg tcggcgaact cgtcggcctc
      721 gacctgcggc ccggcggcgc cggctggatc gacgtcgccg atgccagcgc ccacgtcctg
      781 ggcaagggca gcaagcggcg cagcgtgccg gtcggcggcg cggcgctaaa ggcattgaac
      841 gcgtggttgg cgctgcgagg caatctggcg cgcagcgacg aaccggcgct gctcgtcggc
      901 agccgcggcg cgcggctcgg cgccagccag gtgcgtgcac ggctcaagct gcgcgcgctg
      961 cgcgccggcc tgccgaccca tgtgcacccc cacatgttgc ggcattcatt cgcgacccac
     1021 gtgctgcagt ccagcggcga cctgcgcgcg gtgcaggagc tgctgggcca cgccaacatc
     1081 accaccacgc aggtctacac caagctcgac ttcggccacc tgtccaaggt ctacgacgcg
     1141 gcgcacccgc gcgcgaagaa gcagcgcggc tgaaccgctt gtcgttcagc cgacggccgc
     1201 ggccggcagc ggcacgcgcg tctcgaactt ggcgcggtgc acgctgaaga aggtcttgac
     1261 gttgcgcacg ttcgcgtcct gcgtgaacag gcgctgcacc agcgcgtggt actgcgccat
     1321 gtcgaacacc tgcagcacca gcacgaggtc ggggccgggc gagacgcggt agcactgctg
     1381 cacctcgggc tgcgcgaccg cgcgcgcctc gaactcggcg aggtgctcgg caccctggcg
     1441 gtcgaggctg acctcggcga tcgcggtcag gccggcaccc atcttctgcg gcgacaggat
     1501 cgcgacctgc cgctcgatca cgccgttcgc aaccaggcgc ttgacccggc gcaggcaggt
     1561 tgccgccgag gtgtgcgtcg ccgccgccag cgccaggttc gacagcgagg aatcctgctg
     1621 cagctgggcg aggatgcgca ggtcggccga atcgagagtg accagatcat tcatttatct
     1681 atgatttttg tacgtttcag tcattcctca ttttcaatga aattatgcgc cacttcggcg
     1741 cgatttgtga atgcaatctt ctccccctca cccctacgat cccctcatcg ctttcaacga
     1801 gtagcccacc atgtgcggaa tcgtcggtgc cgtcagcacc cgcaacatcg tcccgatcct
     1861 gatcgagggc ctgaaacgcc tcgagtaccg cggctacgac tcctgcggcg tggcggtgca
     1921 tcagaacggc gagttgcgcc gcgcgcgcag caccgcacgc gtcgccgaac tcgaggccga
     1981 ggtcgcgcgc gacgcggtgc aggccggcac cggcatcgcc cacacccgct gggccacgca
     2041 cggcgcgccg gcggtgcaca acgcgcaccc gcacttcagc cgcggcccgg gcgtgtcgga
     2101 cgcggcagtt gcggtcggcg gcggcggcgc gcaggccggc cggatcgcgc tggtgcacaa
     2161 cggcatcatc gagaaccacg acgagttgcg cgccgagctg caggcccgcg gctacgtctt
     2221 cgcgagccag accgacaccg aggtcatcgc gcacctggtc gaccacctgt acgacggcga
     2281 cctgttcgac gccgtgcagc gcgcgcttcc gcggctgcgt ggcgcct
//
LOCUS       DATMJB010000529         3110 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_705119, whole genome shotgun sequence.
ACCESSION   DATMJB010000529 DATMJB010000000
VERSION     DATMJB010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705119"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /gene="prfB"
                     /locus_tag="VNU48_11410"
     CDS             <1..720
                     /gene="prfB"
                     /locus_tag="VNU48_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HWI11928.1"
                     /translation="NCFIDIQAGAGGTEACDWAGMLLRQYLKYAERKGFKTTLEDETE
                     GDVAGIKSATIKVEGDYAFGLLRTETGVHRLVRKSPFDSAGGRHTSFASLFVYPEVDD
                     TVEIEINPSDVRTDTFRASGAGGQHINKTDSAVRLTHIPTGIVVQCQDGRSQHSNRDV
                     AWKRLRSRLYDHEMRKRMEEQQKLEDTKTDVGWGHQIRSYVLDQSRVKDLRTGYETSN
                     TQKVLDGDLDPFIEASLKQGV"
     gene            816..>3110
                     /gene="pepN"
                     /locus_tag="VNU48_11415"
     CDS             816..>3110
                     /gene="pepN"
                     /locus_tag="VNU48_11415"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HWI11929.1"
                     /translation="MREGTAPLIHRRDYAAPAYWIRSIDLSFDLDPAKTMVVSRMQVE
                     RNTEVAPQPLRLHGEELNLTRVSVNGASVSFRHEDGMLVIDSLQDMPAGAFTLEIRNT
                     CAPEKNTQLSGLYTSGGGFFTQCEAEGFRRITYFLDRPDVMAVYTVLLRANKAKYPVL
                     LSNGNLLEQGELDGGRHFAKWHDPFPKPSYLFALVAADLVARQQRIRTRAGKDHLLQV
                     YVRRGDLDKTEHAMNSLIAAIVWDEARFRLPLDLERFMIVAVADYNAGAMENKGLNLF
                     NTKYVLASPATATDENYAGIESVVGHEYFHNWTGNRITCRDWFQLSLKEGLTVFRDQE
                     FSMDMAGSASARAVKRIQDVRKLRDVQFPEDAGPMAHPVRPESYVAINNFYTATVYEK
                     GAEVVRMMHTLVGREGFAAGMSLYFERFDGRAVTCDDFAQAIADANPASALARTLPQF
                     KRWYSQAGTPRVTARGRYDAPSRTYTLGVEQAGATVDGAAKEPYVIPLAVSLIGRDGR
                     ALPLRLEDEPAGTAATERVLVLEEARSFYTFVDVDVEPVPSLLRGFSAPVILVDGLGD
                     ADLLLLLQHDTDPFNRWEAGQRLALGRLLAAARAEAPFELDAPFIDAMRAVLRHPTLD
                     AAFKDLVLTLPSEGYLAEQLHPVDPPRIHAARMSVVKQLAAALRADWEWAFEAHQVPG
                     PYAPDALSAGKRALANRALAMLCLDSVAGGDSVWPGRAYQRFKDAANMTDRLGALAAL
                     VRSHAPHTEPALYRFHELFRHEPLV"
BASE COUNT          533 a         1119 c         1032 g          426 t
ORIGIN      
        1 aactgcttca tcgacatcca ggccggcgcc ggcggcaccg aggcctgcga ctgggccggc
       61 atgctgctgc gccagtacct gaagtacgcc gagcgcaagg gcttcaagac gacgctggaa
      121 gatgaaaccg agggcgatgt cgccggcatc aagagcgcga ccatcaaggt cgagggcgac
      181 tacgcgttcg gcttgctgcg caccgagacg ggtgtgcacc ggctggtgcg caagagcccg
      241 ttcgactcgg ccggcgggcg ccacaccagc ttcgcgagcc tgttcgtcta ccccgaggtc
      301 gacgacacgg tggagatcga gatcaacccg tcggacgtgc gcaccgacac cttccgcgcc
      361 agcggcgccg gcggccagca catcaacaag accgactcgg cggtgcgcct gacgcacatc
      421 ccgaccggca tcgtcgtgca atgtcaggac ggccgcagcc agcacagcaa ccgcgacgtc
      481 gcctggaagc ggctgcgctc gcgcctgtac gaccacgaga tgcgcaagcg catggaggag
      541 cagcagaagc tcgaggacac caagaccgat gtcggctggg gccaccagat ccgcagctat
      601 gtgctggacc agagccgcgt caaggatctg cgcaccggct acgagaccag caacacgcag
      661 aaggtgctgg acggcgatct cgaccccttc atcgaggcga gcctgaagca aggcgtctga
      721 acagcgcgcc cggcttttcg acacacgatt tctcacccgc tcgggccgca tcgacaacgc
      781 ccccatgagc gcataacaag agagagcccc ccatgatgcg tgaaggcacc gcccccctga
      841 tccaccgccg cgactacgca gcgccggcct actggatccg cagcatcgac ctgagcttcg
      901 acctcgaccc ggccaagacc atggtcgtca gcaggatgca ggtcgagcgc aacaccgagg
      961 tcgcgcccca gccgctgcgc ctgcacggcg aggagctgaa cctgacgcgc gtctcggtga
     1021 acggcgcctc ggtgtcgttc cggcacgagg acggcatgct ggtgatcgat tcgctgcagg
     1081 acatgccggc gggtgcgttc acgctcgaga tccgcaacac ctgcgcgccc gaaaagaaca
     1141 cgcagctctc ggggctgtac acctcgggcg gcggtttctt cacgcagtgc gaggccgagg
     1201 gcttccggcg catcacgtac ttcctcgacc gccccgacgt gatggcggtc tacaccgtcc
     1261 tgctccgcgc caacaaggcg aagtacccgg tgttgctgag caacggcaac ctgctcgagc
     1321 agggcgagct cgacggcggg cgccacttcg cgaaatggca cgacccgttc ccgaagccga
     1381 gctacctgtt cgcgctggtc gcggccgacc tggtcgcgcg ccagcagcgc atccgcacgc
     1441 gcgccggcaa ggaccacctg ctgcaggtct acgtgcgccg cggcgatctc gacaagaccg
     1501 agcacgcgat gaactcgctg atcgcggcca tcgtctggga cgaggcgcgc ttcaggctgc
     1561 cgctggacct ggagcgcttc atgatcgtcg cggtcgctga ctacaacgcc ggcgcgatgg
     1621 agaacaaggg cctgaacctg ttcaacacca agtacgtgct ggcctcgccg gcgaccgcga
     1681 ccgacgagaa ctacgccggc atcgaatcgg tcgtcggcca cgagtacttc cacaactgga
     1741 ccggcaaccg catcacctgc cgcgactggt tccagctcag cctgaaggag ggcctgacgg
     1801 tcttccgcga tcaggaattc agcatggaca tggcgggcag cgccagcgca cgcgccgtca
     1861 agcggatcca ggacgtgcgc aagctgcgcg acgtgcagtt cccggaagac gccggcccga
     1921 tggcgcaccc ggtgcgcccc gagagctacg tagcgatcaa caacttctac accgccacgg
     1981 tctacgagaa gggtgccgag gtcgtgcgca tgatgcacac gctggtcggg cgcgagggct
     2041 tcgccgcggg catgagcctg tacttcgagc gcttcgacgg ccgggccgtg acctgcgacg
     2101 atttcgcgca ggcgatcgcc gacgcgaacc cggccagcgc gctcgcgcgc acgctgccgc
     2161 agttcaagcg ctggtactcg caggccggca cgccgcgcgt gaccgcacgc ggccgctacg
     2221 acgcgccgtc gcgcacctac acgctcggcg tcgaacaggc cggcgcgacg gtcgacggcg
     2281 cggccaagga gccgtacgtc atcccgctgg cggtcagcct gatcggccgc gacggccgcg
     2341 cgctgccgct gcgcctggag gacgagccgg ccggcaccgc cgcaaccgag cgcgtgctcg
     2401 tgctcgaaga ggcacgcagc ttctacacct tcgtcgatgt cgacgtcgag ccggtgccgt
     2461 cgctgctgcg cggcttctcg gcgccggtga tcctggtcga cggcctgggc gatgccgacc
     2521 tgctgctgct gctgcagcac gacaccgacc cgttcaaccg ctgggaagcc ggccagcgcc
     2581 tcgcgctcgg ccggctgctc gccgcggcgc gcgccgaggc gccattcgag ctggacgcgc
     2641 cgttcatcga cgcgatgcgc gcggtgctgc gccaccccac gctggacgcc gcgttcaagg
     2701 acctcgtgct gaccctgccg agcgaaggct atctggccga gcagctccac ccggtcgacc
     2761 cgccgcgcat ccacgccgcg cgcatgagcg tggtgaagca gctcgccgcc gcgctgcgcg
     2821 ccgactggga atgggccttc gaggcgcatc aggtgcccgg cccgtacgcg cccgatgcgc
     2881 tctcggccgg caagcgcgcg ctcgcgaacc gggcgctggc gatgctgtgc ctggactcgg
     2941 ttgccggcgg cgactcggtc tggccgggcc gcgcctacca gcgtttcaag gacgccgcca
     3001 acatgaccga ccggctcggc gcgctggcgg cgctggtgcg ctcgcacgcg ccgcacaccg
     3061 agccggcgct gtaccgcttc cacgagctgt tccgccacga gccactggtg
//
LOCUS       DATMJB010000530         2004 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_358304, whole genome shotgun sequence.
ACCESSION   DATMJB010000530 DATMJB010000000
VERSION     DATMJB010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358304"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VNU48_11420"
     CDS             complement(<1..970)
                     /locus_tag="VNU48_11420"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HWI11930.1"
                     /translation="MLSFIAIVIGGVIGAAWADAAFGGVIGALAGWLSLRSWQHQRQI
                     DALQQALVKLQAGGARAAPGAAPMPVRTAPVETAPAPRVPAAESAPVKAPIDAPTVAS
                     GVAPTVAVTAPSAPRTPVPVLPRPDPLAPIKAWLFGGNTIVKAGVGILFIGLAFLAKY
                     ASEHVQVPVEFRLAGIGGVALVLLVLGWRLRTRRAQYAQILQGGAIAVLYLTLFAAFK
                     FYNVLAVGPAFALMVVVAALAAALAVLQDARSLAVIGALGGFATPLLVSSGGGNQVAL
                     FGYYLVLDLGIAAVAWFRTWRSLNLIGFLFTFVVGTAWGVLDYSPAH"
     gene            1264..>2004
                     /locus_tag="VNU48_11425"
     CDS             1264..>2004
                     /locus_tag="VNU48_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HWI11931.1"
                     /translation="MQATDIHAPDYFHRVVDCQWACPAHTPVPEYIRLIAAGRYTDAY
                     MVNWESNVFPGILGRTCDRPCEPACRRGRVEEAQAAKPEPVAICRLKRVTADFKDDDI
                     SERFPRPALVKNGKRIACVGAGPAALTVARDLVPLGYAVTVFDADTKAGGMIRSQIPR
                     FRLPEEVIDEETGYVLNLGVEFRGGTRIDSLKALIAEGWDAVFVGSGAPRGRDLKIPG
                     RDAAAAHIHIGIDWLSSVSFGHVTRIGKR"
BASE COUNT          342 a          736 c          679 g          247 t
ORIGIN      
        1 agtgggcggg gctgtagtcg agcacgcccc aggccgtgcc gacgacgaag gtgaacagga
       61 agccgatcag gttcagcgag cgccaggtgc ggaaccaggc gaccgccgcg atgccgaggt
      121 cgagcaccag gtagtagccg aacagggcga cctggttgcc gccgccgctg gacaccagca
      181 gcggcgtcgc gaagccgccg agcgcgccga tgacggcgag cgagcgggcg tcctgcagga
      241 ccgcgagcgc ggcggccagc gcggccacca cgaccatcag cgcgaacgcc ggtccgaccg
      301 caagcacgtt gtagaacttg aaggcggcga agagcgtcag gtacagcacc gcgatcgccc
      361 cgccctgcag gatctgcgcg tactgtgcgc gtcgcgtgcg cagccgccag ccgagcacca
      421 gcagcacgag cgcgacgccg ccgatcccgg cgaggcggaa ctcgaccggc acctggacat
      481 gctcgctcgc gtacttcgcc aggaaggcca ggccgatgaa caggatgccc acgcccgcct
      541 tgacgatcgt gttgccgccg aacagccagg ccttgatcgg cgcgagcggg tcggggcggg
      601 gcagcaccgg caccggggtt ctgggagcgc tcggggccgt cacggcgacc gttggcgcga
      661 cgcccgacgc gacggtgggc gcatcgatcg gcgctttcac cggcgccgat tcggcggccg
      721 gcacgcgcgg cgctggcgcg gtctcgaccg gcgcggtgcg aaccggcata ggcgcggcgc
      781 ctggcgcagc gcgggcaccg ccagcctgca atttcaccag cgcctgctgc agtgcatcga
      841 tctgccgctg gtgctgccac gagcgcagcg acagccaccc ggccagcgca ccgatgacgc
      901 caccgaacgc agcatcggcc cacgcggcgc cgatcacgcc gccgatcacg atcgcgatga
      961 aactcagcac ggcccctccc tgttgtgatt cagatggctg cgattcactc tagcagggcc
     1021 ctgcggccgg accccacccc catcggagcg aggccgccgc ggtatcgagc agcgcccgct
     1081 gcagcagcgt ctcacgacac cgcagcgcac cgaaacgagt aaacctgccg gacccgggca
     1141 ccacactggc gcaaaccctc gctgacagtg tgccggcgca ggcctagcat catcggcacc
     1201 gcagcaagcg cgttggcccg gcagcacgac caccggattc gagcgcgcgg aaagagaccc
     1261 tcgttgcaag ccaccgatat ccacgccccc gactacttcc acagagtggt cgactgccag
     1321 tgggcctgcc ctgcccacac cccggttccc gaatacatcc gcctgatcgc ggccggccgc
     1381 tacaccgatg cctacatggt caactgggaa tcgaatgtgt tcccgggcat cctcggccgc
     1441 acctgcgacc gcccctgcga gccggcgtgc cggcgcggcc gcgtcgaaga ggcgcaggcc
     1501 gcgaagcccg agccggtcgc gatctgccgc ctgaagcgcg tcaccgccga cttcaaggac
     1561 gacgacatct ccgagcgttt tccgcgcccc gcgctggtga agaacggcaa gcgcatcgcc
     1621 tgcgtcggcg ccggcccggc agcgctgacg gtggcgcgcg acctcgtccc gctcggctac
     1681 gcggtcacgg tgttcgacgc cgacacgaaa gccggcggca tgatccgctc gcagatcccg
     1741 cgcttccgtc tgcccgagga ggtgatcgac gaagagaccg gctatgtgct gaacctgggc
     1801 gtcgagttcc gcggcggcac acgcatcgac tcgctgaagg ccttgatcgc cgaaggctgg
     1861 gacgcggtct tcgtcggctc gggcgcaccg cgcggccgcg acctgaagat ccccgggcgc
     1921 gacgcagccg cggcgcacat ccacatcggc atcgactggc tttcatcggt gtcgttcggc
     1981 cacgtcacgc gcatcggcaa gcgg
//
LOCUS       DATMJB010000531         2398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_199306, whole genome shotgun sequence.
ACCESSION   DATMJB010000531 DATMJB010000000
VERSION     DATMJB010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199306"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VNU48_11430"
     CDS             complement(<1..521)
                     /locus_tag="VNU48_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11932.1"
                     /translation="MIVEGLHRRRADGFSLFQLAGNRRELLGCAAKGRVYSLSRIKDD
                     SSFETHGLSRRTVDTLAEASHSALSGVARVLVHAGRLNAKVAEDLVRSSKDKRNSFVA
                     SVIAAGAVSPADLAHTLSNALALPLLDLSSVDPQRLPRNVIDAKLAAQYQVVVLGKRG
                     SRLFIGGADPTDQ"
     gene            complement(663..739)
                     /locus_tag="VNU48_11435"
     tRNA            complement(663..739)
                     /locus_tag="VNU48_11435"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(703..705),aa:Pro,seq:cgg)
     gene            complement(779..1720)
                     /gene="ispB"
                     /locus_tag="VNU48_11440"
     CDS             complement(779..1720)
                     /gene="ispB"
                     /locus_tag="VNU48_11440"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HWI11933.1"
                     /translation="MAADMREVDGVIRQRLASDVAMINQIAHYIISAGGKRIRPMLVL
                     LFSNALGFAGRERFELAATVEFIHTATLLHDDVVDESALRRGRQTANALFGNAASVLV
                     GDFIYSRAFQMMVSVNRLRVLEVLADATNVIAEGEVLQLMNMHDPDLSVDDYLRVIRF
                     KTAKLFEASARVGAVLAEAGASVEESCAAYGRSLGTAFQLIDDLLDYEGATEQLGKNV
                     GDDLREGKPTLPLLLAMERGSASERDLIRHAIEHGEVARLPEIVEIVRRTGAIAATRD
                     AARTEADKAARALNVLPQSAYREALLELCFRSVERSS"
     gene            2005..2316
                     /gene="rplU"
                     /locus_tag="VNU48_11445"
     CDS             2005..2316
                     /gene="rplU"
                     /locus_tag="VNU48_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWI11934.1"
                     /translation="MYAVIKTGGKQYKVAAGEKIKIEQIAADVGQEITIDQVLAIGAG
                     ADLTVGTPLVTGASVVATVVAHGRHDKVRIFKMRRRKHYQKRQGHRQNFTEIQIGAVN
                     A"
BASE COUNT          479 a          741 c          779 g          399 t
ORIGIN      
        1 tcctggtcgg tcgggtcggc gccgccgatg aacagccgac tgccgcgctt gcccaggacc
       61 accacctggt actgcgccgc gagcttggcg tcgatcacgt tgcgcggcag ccgctgcggg
      121 tcgaccgacg acaggtcgag caagggcagc gcgagcgcat tcgacagcgt gtgcgcgaga
      181 tcggccggcg acaccgcgcc ggcggcgatc accgaggcga cgaagctgtt gcgcttgtcc
      241 ttggaactgc gaaccagatc ctcggcgacc ttcgcattca gcctgccggc gtgcacgaga
      301 acccgtgcga cgcccgagag cgcggaatgc gaagcttctg ccaatgtatc cacggttcgc
      361 cggctcagtc catgcgtttc aaacgaagaa tcatccttga tccgtgaaag actgtaaacg
      421 cgcccttttg ccgcgcagcc caacaattca cgcctgttcc cggccaattg aaacagactg
      481 aatccatcgg ctcggcggcg atgaaggcct tcgacgatca agggatggcc ggaaaatcaa
      541 cagattagca cgcgatctgc aacaaaggcc ttcgcaacgg gcgaatcgca ggccaagccc
      601 gcgaaagcgc cgctctgcgc ggcgtgcacg caacgaacag gatgtttggg ggacggtgag
      661 gatggtcggg gtgagaggat tcgaacctcc ggcctctacg tcccgaacgt agcgctctac
      721 caggctaagc tacaccccga ttctctggct gcgtgaagca aggccaacgc cgaagcactc
      781 aggaagagcg ttcgacggat cgaaaacaca attctaacag agcttcccgg taagccgatt
      841 gcggcaatac gttcagggct cgagcggcct tgtcggcttc cgtgcgggcc gcgtcgcgcg
      901 tggccgcgat cgcgccggta cgacgcacga tctcgacgat ctcgggcagg cgcgcgacct
      961 cgccgtgctc gatcgcgtgg cggatcagat cgcgctcgct cgcgctgccg cgctccatcg
     1021 ccagcagcaa gggcagggtt ggcttgcctt cgcgcaggtc gtcgccgacg ttcttgccga
     1081 gctgctcggt ggcgccttcg tagtcgagca gatcgtcgat cagctggaac gcggtgccca
     1141 gcgaacggcc gtaggcggcg cacgattctt cgacgctcgc gccggcctcg gcgagcacgg
     1201 cgccgacgcg cgcgctggcc tcgaacaact tggcggtctt gaagcggatc acgcgcaggt
     1261 agtcgtccac cgacagatcg gggtcgtgca tgttcatcag ctgcagcact tcgccctcgg
     1321 cgatcacgtt ggtcgcgtcc gccagtacct cgagcacgcg caaccggttc accgaaacca
     1381 tcatctggaa cgcgcgcgaa tagatgaagt cacccaccag cacgctcgcc gcgttgccga
     1441 acagtgcgtt ggccgtctgc cggccgcggc gcagcgcaga ctcgtcgacc acgtcgtcgt
     1501 gcagcagcgt ggccgtgtga atgaactcca cggtcgcggc cagttcgaaa cgctcgcgcc
     1561 cggcaaagcc gagcgcattg gaaaacagca gcaccagcat cggacggatc cgcttgccgc
     1621 cggcactgat gatgtagtgg gcgatctggt tgatcatcgc cacatcggag gcgaggcgtt
     1681 gccggatcac gccgtccact tcccgcatgt cggcggccat cagagccgca gcgccgggga
     1741 gcggtgcgtc gacgacaggc gggtgagaag gcggagagtc tgagtgcacg ctgggctcgt
     1801 ttgaccgggg gtgattatag aaactgggtc ggccgcgcga gcggattcac ctcaaaaatg
     1861 cgccccatgc tagaatcaca ggctctgcgc attcggcagg gctgcctggt cgaggattgt
     1921 gccttgatcc cgggcacgtt ggacgatcac cctcgggcgg tcgagttgga ggccgccgca
     1981 ggcggctgca ctgaaagggc tgatatgtac gcggtcataa aaaccggtgg caagcaatac
     2041 aaggttgctg ctggcgaaaa gatcaaaata gaacagattg ctgcggatgt tggccaagag
     2101 atcacgatcg accaggtgtt ggccattggt gccggcgccg atttgacggt cgggactccc
     2161 ttggtaacgg gcgcaagcgt ggtggccacc gtggtggctc atggcaggca tgacaaggtg
     2221 cgcatcttca agatgcgccg tcgcaaacac tatcaaaagc ggcagggtca ccgtcagaac
     2281 ttcaccgaga tccaaatcgg tgcggtgaac gcgtaagcgc gcttgtcgtg acctaaccca
     2341 gtcaaggagc agaaaccatg gcacagaaaa agggcggcgg ttccactcgg aacggccg
//
LOCUS       DATMJB010000532         2489 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_198561, whole genome shotgun sequence.
ACCESSION   DATMJB010000532 DATMJB010000000
VERSION     DATMJB010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198561"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1958
                     /locus_tag="VNU48_11450"
     CDS             <1..1958
                     /locus_tag="VNU48_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HWI11935.1"
                     /translation="PALEGHYELADGTKVAPAFQLLRERVASCTPEWAAAITGIGADR
                     IRQLAREMGDTALKQAFELPIPWTDAWGKLHPTTPARPVAFHAMRGLAAHSNGFQTVR
                     ALAVLMSVLGTIDAPGGFRHKAPYPRHIVPNYRAFNSPEMIQPNTPLNAAPLGFPANP
                     DELAINPDGSPIRIDHAFSWEHPLSAHGLMHNVITNAVKGDPYRIDTLLIFMANMAWN
                     SSMNTMAVREMLNRKDEHGEHMIPFLVVCDAFQSETVAFADLVLPDTTYLERHDVMSM
                     LDRPISEFDGPVDSVRVPVLPVTGQCKPFQEVLIELASRLEFPAFTTEQGTRKFKDYP
                     EFIVNFQPQPGIGFLMGWRGVNGDKQLRGEPNPNQWAMYAKNDCVFQYHMPETMHYMR
                     NWNREYLDFAKDKGWRQKNDPVQLAIYSDTLQSFRLAAQGKSAGRQPPEHLRARIDTY
                     FDPLPFWYAPLEEAATDLAAYPLNAITQRPMAMYHSWDSQNAWLRQIHSHNYLQVNPL
                     TARAAGIEDGGWCWVESQWGKVRCMLRYSEAVEPGTVWTWNAIGKADGAWQLAPGADE
                     ARKGFLLNHLISEELPFAGSATKTISNSDPVTGQAGWYDVRVRIRPAEPEEPEVTSPQ
                     IATVPAAPGVRGHAARVLTYFAGRKTS"
     gene            1955..>2489
                     /locus_tag="VNU48_11455"
     CDS             1955..>2489
                     /locus_tag="VNU48_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWI11936.1"
                     /translation="MIRWLQDLLQTNAVADAPARNAPPAKPAPPPSARALPGETLAKQ
                     LALVIDLNVCVGCHACVTSCKEWNTSGTAGPLVDANAYGANPTGTFFNRVQTYEAGIF
                     PGTETIHFPKSCLHCEDPPCVPVCPTGASYKRKEDGIVLVDYDKCIGCKYCAWACPYG
                     VREIDEHQKVMKKCTLCV"
BASE COUNT          457 a          890 c          799 g          343 t
ORIGIN      
        1 acccggcgct cgaagggcac tacgaactcg ccgacggcac caaggtcgcg cccgcattcc
       61 agctgctgcg cgagcgcgtc gcgagctgca ccccggagtg ggccgccgcg atcaccggca
      121 tcggcgccga ccgcatccgc caactcgcgc gcgagatggg cgacaccgcg ctgaagcagg
      181 ccttcgagct gccgatcccg tggaccgacg cgtggggcaa gctgcacccg acgacgcccg
      241 cacgcccggt cgcgttccac gcgatgcgcg gcctcgcggc gcactcgaac ggcttccaga
      301 cggtgcgcgc gctggcggtg ctgatgagcg tgctcggcac gatcgacgcg cccggcggct
      361 ttcgccacaa ggcgccgtac ccgcgccaca tcgtgccgaa ctaccgcgcg ttcaactcgc
      421 ccgagatgat ccagccgaac acgccgctga acgcggcgcc gctcggcttt ccggcgaacc
      481 cggacgagct cgcgatcaac ccggacggct cgccgatccg catcgaccac gcgttctcgt
      541 gggagcaccc gctgtcggcg cacgggctga tgcacaacgt gatcacgaac gcggtcaagg
      601 gcgacccgta ccgcatcgac accttgctga tcttcatggc caacatggcc tggaacagca
      661 gcatgaacac gatggccgtg cgcgagatgc tgaatcgcaa ggacgaacac ggtgagcaca
      721 tgatcccgtt cctcgtcgtc tgcgacgcgt tccagagcga gaccgtcgcg ttcgccgacc
      781 tggtgctgcc cgacaccacc tacctcgagc gccacgacgt gatgagcatg ctcgaccggc
      841 cgatctcgga gttcgacggc ccggtcgact cggtgcgcgt gccggtgctg ccggtcaccg
      901 gccagtgcaa gccgttccag gaggtgctga tcgaactcgc gtcgcgcctg gagttcccgg
      961 cgttcaccac cgagcagggc acgcgcaagt tcaaggacta ccccgagttc atcgtcaact
     1021 tccagccgca gcccggcatc ggcttcctga tgggctggcg cggcgtgaac ggcgacaagc
     1081 agctgcgcgg cgaaccgaac ccgaaccagt gggcgatgta cgccaagaac gattgcgtct
     1141 tccagtacca catgcccgaa accatgcact acatgcgcaa ctggaaccgc gagtacctgg
     1201 acttcgcgaa ggacaagggc tggcgccaga agaacgaccc ggtgcagctc gcgatctaca
     1261 gcgacacgct gcagagtttt cgtctcgcgg cgcagggcaa gagcgcgggc cggcaaccgc
     1321 ccgagcacct gcgcgcgcgc atcgacacat acttcgaccc gctgccgttc tggtacgcac
     1381 cgctcgaaga ggccgcgacc gacctggccg cgtacccgct gaacgcgatc acgcagcggc
     1441 cgatggcgat gtaccactcc tgggactcgc agaacgcgtg gctgcgccag atccacagcc
     1501 acaactacct gcaggtgaac ccgctgaccg cgcgcgccgc gggcatcgaa gacggcggct
     1561 ggtgctgggt cgaaagtcaa tggggcaagg tgcgttgcat gctgcgctac agcgaggcgg
     1621 tcgagcccgg caccgtctgg acctggaacg cgatcggcaa ggccgacggc gcctggcaac
     1681 tcgcgcccgg cgccgacgag gcgcgcaagg gctttttgct gaaccacctg atcagcgagg
     1741 agctgccgtt tgccggcagt gccacgaaga cgatcagcaa ctccgatccg gtcaccggcc
     1801 aggcgggctg gtacgacgtg cgtgtgcgca tccggcccgc cgagcccgag gagcccgaag
     1861 tcacctctcc gcagatcgcg accgtgccgg ccgcgcccgg cgtgcgcggt cacgcggcac
     1921 gcgtgctcac ctacttcgcc ggacgcaaga catcatgatc cgctggctgc aagacctgct
     1981 gcaaacgaat gccgtcgccg acgcgccggc gcgcaacgcg ccaccagcca agcctgcacc
     2041 gccacccagc gcgcgcgcct tgcccggcga gacgctggcc aagcagctcg cgctggtgat
     2101 cgacctgaac gtctgcgtcg gctgccacgc ctgcgtgacc tcgtgcaagg agtggaacac
     2161 ctcgggcacc gccgggccgc tggtcgacgc aaacgcctac ggtgcgaacc cgaccggcac
     2221 cttcttcaac cgcgtgcaga cctacgaggc cggcatcttc cccggcaccg agacgatcca
     2281 ctttccgaag agctgcctgc attgcgaaga cccgccgtgc gtgccggtat gcccgaccgg
     2341 cgcgagctac aaacgcaagg aggacggcat cgtgctggtc gactacgaca agtgcatcgg
     2401 ctgcaagtac tgcgcgtggg cgtgccctta cggcgtgcgc gagatcgacg agcaccagaa
     2461 ggtcatgaag aagtgcacgc tgtgcgtcg
//
LOCUS       DATMJB010000533         3618 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_732653, whole genome shotgun sequence.
ACCESSION   DATMJB010000533 DATMJB010000000
VERSION     DATMJB010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732653"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VNU48_11460"
     CDS             complement(<1..188)
                     /locus_tag="VNU48_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11937.1"
                     /translation="MPAPKDTEPGGAPTQPPSLPADNALWDEADMNLMHGPAASFVDV
                     SLDAPGAPVPTIGHIGRYA"
     gene            complement(316..1515)
                     /locus_tag="VNU48_11465"
     CDS             complement(316..1515)
                     /locus_tag="VNU48_11465"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWI11938.1"
                     /translation="MRLARRLDHIEPFYVMECAKAAVELARSPACDARLGGQPMIFLN
                     IGEPDFTAPVLVQEAAERALRAGRTQYTDATGLPALRERISAWYAQRFDLSIAPRRIV
                     VTAGASAALQLACLALVDRDDEVLLPDPSYPCNRHFVAAAEGVPVLLPAGAEARWQLD
                     AASVAAAWTPRTRGVLLASPSNPTGTSIDPDEMGRIVSAVRARGGFALVDEIYLGLSF
                     DERFGHSALVHDKSGHGDDVIAINSFSKYFSMTGWRLGWLVVPETLVPAIEKLAQNLY
                     ICASSVAQHAALACFEPDSIAEYERRRDEFRARRDFLVPALDALGLTVPVMPDGAFYA
                     WADCSAYNPSSWDFCFDMMRRAHVAITPGRDFGRAVSERFVRLSYASSMAQLHEAVER
                     LRHALRN"
     gene            complement(1526..2047)
                     /gene="nusB"
                     /locus_tag="VNU48_11470"
     CDS             complement(1526..2047)
                     /gene="nusB"
                     /locus_tag="VNU48_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWI11939.1"
                     /translation="MTDPVVPEETAAVSTPPKKKSGARPARPKSARRRSREVALQGLY
                     EWLVSGAEASAIDAHMHEQDGFDKCDSVHFDALLHGCIDEAVTIDAVLARHVDRKTTQ
                     LSPIEHGVLMIGVYELTHCIEIPYKVAINEAVELAKSFGGTDGHKYVNGVLDKAAADL
                     RPAEVEAMRAARR"
     gene            complement(2044..2526)
                     /gene="ribH"
                     /locus_tag="VNU48_11475"
     CDS             complement(2044..2526)
                     /gene="ribH"
                     /locus_tag="VNU48_11475"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWI11940.1"
                     /translation="MQDADQGDTRSGGLQAPIDGRDLRIGIVQARFNAALTDRLAQAC
                     LAELQALGVAARHIKHVTVPGALEIALALHALADTDDCDALIALGCIVRGETYHFELV
                     ANESGAGVTRVALDHAVPIANAILTVENEAQAWARAEDKGRDAARVAVEMANLMEALS
                     "
     gene            complement(2542..>3618)
                     /gene="ribBA"
                     /locus_tag="VNU48_11480"
     CDS             complement(2542..>3618)
                     /gene="ribBA"
                     /locus_tag="VNU48_11480"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HWI11941.1"
                     /translation="VAELAAGRMIILVDEEDRENEGDLLIAADHVTPEAINFMARFGR
                     GLICLTLTPERCDRLQLHSMTARNGTRHGTAFTVSIEAATGVDTGISAADRARTVQAA
                     VARDAKASDLVQPGHIFPLRAQEGGVLMRAGHTEAGCDLAGMAGLTSAAVICEVMKDD
                     GTMARLPDLIAFGRQHGLKIGTIADLIEYRSRHESLIERVGTRPLVTPQGEFECAAFR
                     DRTGGLHLALTKGRWTEADEVLVRVHEPLSVMDLLDVGRPCQHSWPLPKALALLHKGP
                     RGAALLLNCGEDVAALVPHVLPLQEAGTRQPGQMDLRTYGIGAQILRELGVAKMKLLG
                     SPRRMPSMTGYGLEVTGFLAAEPQ"
BASE COUNT          488 a         1262 c         1315 g          553 t
ORIGIN      
        1 gcgtagcggc cgatgtggcc gatcgtcgga accggggcgc cgggggcgtc gagcgacacg
       61 tcgacgaagc tcgcggccgg gccgtgcatc aggttcatgt cggcctcgtc ccacagcgcg
      121 ttgtccgcag gcaggctcgg cggctgggtg ggcgcgccgc cgggctcggt gtctttcggc
      181 gcgggcacgg gaggtggggt gagcgaggtc accgcgtcag cttagcggag tgcctgtggc
      241 acaaacgcac gaaaagcccc ctcattgccg gcttcgtcac acacgctcac gattcggccg
      301 gtcgttgcgc ccggctcagt ttcgcagcgc gtggcgcagg cgctcgacgg cctcgtgcag
      361 ctgcgccatc gaactcgcgt acgacagccg cacgaaacgc tcggagactg cgcggccgaa
      421 gtcgcgcccc ggcgtgatcg cgacgtgggc gcgcctcatc atgtcgaagc agaagtccca
      481 actgctcggg ttgtacgccg agcaatcggc ccaggcgtag aacgcgccgt cgggcatcac
      541 cggcacggtc aggccgagcg cgtccagcgc cggcacgaga aagtcgcggc gcgcgcggaa
      601 ctcgtcgcgc cggcgctcgt actcggcgat cgagtcgggc tcgaagcagg ccagcgccgc
      661 gtgctgcgcg acgctggacg cgcagatgta caggttctgc gcgagcttct cgatcgccgg
      721 caccagcgtc tcgggcacga ccagccagcc caggcgccag ccggtcatcg agaagtattt
      781 cgagaagctg ttgatcgcga tcacatcgtc gccgtgcccg ctcttgtcgt ggaccagcgc
      841 gctgtggccg aagcgttcgt cgaagctcag gccgaggtag atctcgtcga ccagcgcgaa
      901 gccgccgcgc gcgcgcaccg cgctcacgat gcggcccatc tcgtcggggt cgatcgaggt
      961 gccggtcggg ttcgacggcg aggccagcag cacgccgcgg gtgcgcggcg tccaggcggc
     1021 ggccacgctc gcggcatcga gctgccagcg cgcctcggcg ccggccggca gcagtaccgg
     1081 caccccttcg gccgcggcga cgaagtgccg gttgcagggg tagctcggat cgggcagcag
     1141 cacctcgtcg tcgcggtcga cgagcgccag gcaggcgagc tgcagcgcgg ccgacgcgcc
     1201 tgcggtcacg acgatgcggc gcggcgcgat gctcaggtcg aaacgctgcg cgtaccacgc
     1261 gctgatgcgc tcgcgcagcg ccggcagacc ggtcgcgtcg gtgtactgcg tgcggcccgc
     1321 gcgcagcgcg cgctcggccg cttcctgcac gagcaccggc gcggtgaagt cgggctcgcc
     1381 gatgttcagg aagatcatcg gctggccgcc caggcgcgcg tcgcacgccg ggctgcgcgc
     1441 cagctcgacc gcggccttgg cgcactccat cacgtagaaa ggctcgatgt ggtcgagccg
     1501 ccgagcgagc ctcatgcgcg cgatctcagc gccgcgccgc gcgcatcgcc tcgacttccg
     1561 cggggcgcag gtcggcggcg gccttgtcga gcacgccgtt gacgtacttg tggccgtcgg
     1621 tgccgccgaa actcttcgcg agttcgaccg cctcgttgat cgcgaccttg tacgggatct
     1681 cgatgcagtg cgtcagctcg tagacgccga tcatcagcac gccgtgctcg atcggcgaga
     1741 gctgcgtggt cttgcggtcg acgtggcgcg cgagcacggc gtcgatcgtg acggcctcgt
     1801 cgatgcagcc gtgcagcagc gcatcgaagt gcaccgaatc gcacttgtcg aagccgtcct
     1861 gctcgtgcat gtgggcgtcg atcgcgctcg cctcggcgcc cgacaccagc cattcgtaca
     1921 ggccttgcag cgccacttcg cgcgaacgcc ggcgcgccga cttcggccgc gcgggccggg
     1981 cgccggactt cttcttcggc ggcgtcgaga cggcggcggt ttcctcgggc acaacggggt
     2041 cggtcacgac agcgcttcca tcaggttggc catctcgacc gcgacgcggg cggcgtcgcg
     2101 ccccttgtct tcggcgcgcg cccaggcctg tgcttcgttc tcgaccgtca ggatcgcgtt
     2161 ggcgatcggc accgcgtggt cgagtgcgac ccgcgtcacg ccggcgccgc tctcgttggc
     2221 gacgagctcg aagtggtagg tctcgccgcg cacgatgcag cccagcgcga tcagcgcgtc
     2281 gcagtcgtcg gtgtcggcca gcgcgtgcag cgcgagcgcg atctcgagcg cgcccggcac
     2341 ggtcacgtgc ttgatgtgcc tggcagcgac gcccagcgcc tgcaactcgg ccaggcaggc
     2401 ctgcgcgagc ctgtcggtga gcgcggcatt gaagcgcgcc tggacgatgc cgatgcgcag
     2461 gtcgcggccg tcgatcggcg cctgcagacc gccgctgcgg gtgtcgccct gatcagcgtc
     2521 ttgcatgtcg agtcgccttc atcattgtgg ttccgcggcg aggaagccgg tgacctcgag
     2581 accgtagccg gtcatgctgg gcatgcggcg cgggctgccg agcagcttca tcttcgcgac
     2641 gccgagttcg cgcagaatct gcgcgccgat gccgtaggtg cgcaggtcca tctggccggg
     2701 ctggcgggtg ccggcttcct ggagcggcag cacgtgcggc accagcgcag cgacgtcttc
     2761 gccgcagttg agcagcagcg ccgcgccgcg cgggcccttg tgcagcaacg cgagcgcctt
     2821 gggcagcggc cacgaatgct ggcagggccg gccgacgtcg agcaggtcca tcaccgacag
     2881 cggctcgtgc acgcgcacca gcacctcgtc ggcttcggtc cagcggccct tggtcagcgc
     2941 caggtgcagg ccgccggtgc ggtcgcgaaa cgccgcgcac tcgaactcgc cctgcggcgt
     3001 gaccagcggt cgggtgccga cgcgctcgat cagcgattcg tgccggctgc ggtattcgat
     3061 caggtcggcg atcgtgccga tcttcaggcc gtgctgccgg ccgaacgcga tcaggtcggg
     3121 caggcgcgcc atcgtgccgt cgtccttcat gacctcgcag atcaccgcgg cgctggtcag
     3181 gccggccatg ccggcgaggt cgcagccggc ctcggtgtga ccggcgcgca tcagcacgcc
     3241 gccttcctgc gcacgcagcg ggaagatgtg gccgggctgc accaggtcgc tggccttcgc
     3301 gtcgcgcgcg accgcggcct gcacggtgcg cgcgcgatcc gcggccgaga tgccggtgtc
     3361 gacgccggtg gcggcctcga tcgacaccgt gaacgcggtg ccgtggcgcg tgccgttgcg
     3421 tgccgtcatc gagtgcagct ggagccggtc gcagcgttcg ggcgtcagcg tcaggcagat
     3481 caggccgcgg ccgaagcgcg ccatgaagtt gatcgcctcg ggggtgacgt ggtcggccgc
     3541 gatcagcaga tcgccttcgt tctcgcggtc ctcctcgtcg acgaggatga tcatgcggcc
     3601 cgcggccagc tcggcgac
//
LOCUS       DATMJB010000534         2288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_838020, whole genome shotgun sequence.
ACCESSION   DATMJB010000534 DATMJB010000000
VERSION     DATMJB010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838020"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1315
                     /locus_tag="VNU48_11485"
     CDS             56..1315
                     /locus_tag="VNU48_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWI11942.1"
                     /translation="MLTSRNVPSHCAGASAVVIAVSLLAGCGPKAEPGGGAPGGGMPP
                     AQVGVITVTPQTIGLATELPGRLEASRVAQVRARAAGILQKRVFTEGSDVKAGQLLFR
                     IDAASYEASAASARAALARAQANLTQAAALAERYRPLLEANAISKQEYVNAVAAQKTA
                     EADVATGRANLQTAQINLGYATVTAPISGRIGRALVTEGALVGQGDATPLATIQQINP
                     MYVNFTQSTTDVLRLRKAVESGKFRRVGGDDLGAAGAGALPVRVILEDGSDYGQPGKL
                     LFSDLTVDPTSGQITLRAEVPNPTGLLLPGMYVRVRLEQARAEAGIVVPQQAVTRGST
                     GDSVMVVGADGKVAPRPVKIGSSVSGQWVVLDGLKPGEQVMVDGFQKLRGNAPVKPVP
                     WNPAASAASAAGAASAASGPAAAASAK"
     gene            1328..>2288
                     /locus_tag="VNU48_11490"
     CDS             1328..>2288
                     /locus_tag="VNU48_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWI11943.1"
                     /translation="MAQFFIDRPVFAWVIALFILVLGGVAIRQLPVAQYPTVAPPSIV
                     LTATYPGASAQTLEDSVISVIEQEMNGSPGLIYMESVSQANGVGSITLTFESGTDASL
                     AQVDVQNRLSRAAPRLPAAVTQQGVRVDKARSNFLLVTILSSDNPAYDPIALGDYASR
                     TVLPEIQRIPGVGQAQLFGTERSMRIWIDPAKLVGYGLTPNDVNAAIRAQNAQVSSGT
                     IGDLPNVPGQSISATVVVNGQLVSEREFGNILLRANTDGSTVRLRDVARIELGGQNYG
                     TSARLNGKPSAGIGVQLSPTGNALATATRVRARVTELAQYFPPG"
BASE COUNT          334 a          827 c          817 g          310 t
ORIGIN      
        1 cgatccaccc ctcctgccgt cgccccgacg tgcgccatgt cctgaagggt tctgcatgtt
       61 gacatcgagg aacgtgccgt cgcattgcgc tggcgcaagt gccgtcgtga tcgccgtgtc
      121 gctgctggcc ggctgcgggc cgaaggccga gccgggcggc ggcgcgcccg gcggcggcat
      181 gccgccggcc caggtcggcg tgatcacggt gacgccgcag accatcggcc tggcgaccga
      241 gctgccgggc cggctcgagg cctcgcgcgt cgcgcaggtg cgggcccgcg ccgccggcat
      301 cctgcaaaag cgcgtgttca ccgaaggcag tgacgtgaag gccgggcagc tgctgttccg
      361 catcgacgcc gcgtcctacg aggcgagcgc ggcgagcgcg cgggccgcgc tggcgcgcgc
      421 gcaggcgaac ctgacgcagg ccgcggcgct cgccgagcgc taccggccgc tgctcgaggc
      481 gaacgcgatc agcaagcagg agtacgtcaa cgccgtggcg gcgcagaaga ccgccgaggc
      541 cgacgtggcc accggccgcg ccaatctgca gacggcgcag atcaatctcg gctatgcgac
      601 cgtcacggcg ccgatctcgg ggcgcatcgg ccgcgcgctc gtcaccgagg gcgcgctggt
      661 cggccagggc gacgcgacgc cgctggcgac gatccagcag atcaacccga tgtacgtgaa
      721 cttcacgcag tcgacgaccg acgtgctgcg cctgcgcaag gcggtcgaga gcggcaagtt
      781 caggcgcgtc ggcggcgatg acctcggcgc cgcgggcgcc ggtgcgctgc cggtgcgcgt
      841 gatcctcgaa gacggcagcg actacggcca gcccggcaag ctgctgttct ctgacctgac
      901 ggtcgacccg acctcgggcc agatcacgct gcgcgccgag gtgccgaacc cgaccggcct
      961 gctgctgccc ggcatgtacg tgcgcgtgcg cctcgagcag gcccgggccg aggccggcat
     1021 cgtggtgccg cagcaggccg tgacgcgcgg ctcgaccggc gactcggtga tggtcgttgg
     1081 cgccgacggc aaggtggcac cgcggccggt caagatcggc tcgtcggtga gcggccagtg
     1141 ggtcgtgctc gacggcctga agccgggcga gcaggtcatg gtcgacggct tccagaagct
     1201 gcgcggcaac gcgccggtca agcctgtgcc gtggaatccg gcggcgagcg ccgcgtccgc
     1261 agcaggcgcg gcgtctgcgg ccagtggccc ggccgcggcc gcgtcggcga agtaaggggc
     1321 gcaccccatg gcccagttct tcatcgatcg accggtcttc gcgtgggtga tcgcgctgtt
     1381 catcctggtg ctcggtggcg tggccatcag gcagctgccg gtcgcgcaat acccgaccgt
     1441 cgccccgccc tcgatcgtcc tcaccgccac ctacccgggt gcctcggcgc agaccctcga
     1501 agacagcgtg atcagcgtca tcgagcagga gatgaacggc tcgcccggcc tgatctacat
     1561 ggagtcggtg agccaggcca acggtgtcgg ctcgatcacg ctgaccttcg agtcgggcac
     1621 cgacgcaagc cttgcgcagg tcgacgtgca gaaccggctg agccgcgccg cgccgcgcct
     1681 gcctgccgcc gtgacgcagc agggcgtgcg cgtcgacaag gcgcgctcca acttcctgct
     1741 ggtcacgatc ctgtcgagcg acaacccggc ctacgacccg atcgcgctcg gcgactacgc
     1801 ctcgcgcacc gtgctgcccg agatccagcg catccccggg gtcggccagg cgcagttgtt
     1861 cggcaccgag cgctcgatgc ggatctggat cgatccggcc aagctggtcg gctacggcct
     1921 cacgccgaac gacgtgaacg cggccatccg cgcgcagaac gcacaggtct cgtcgggcac
     1981 gatcggtgat ctgcccaacg tgccggggca gagcatctcg gcgaccgtcg tcgtcaatgg
     2041 ccagctcgtc tcggagcgcg agttcggcaa catcctgctg cgcgccaata ccgacggctc
     2101 gacggtgcgg ctgcgcgatg tcgcccgcat cgagctcggc gggcagaact acggcacctc
     2161 ggcgcgcctg aacggcaagc cctccgccgg catcggcgtg cagctctcgc cgaccggcaa
     2221 cgcgctcgcc accgccacgc gggtgcgcgc gcgcgtgacc gaactggcgc agtacttccc
     2281 gcccggcg
//
LOCUS       DATMJB010000535         3835 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_863186, whole genome shotgun sequence.
ACCESSION   DATMJB010000535 DATMJB010000000
VERSION     DATMJB010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863186"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="VNU48_11495"
     CDS             complement(<1..1051)
                     /locus_tag="VNU48_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HWI11944.1"
                     /translation="MTDPLVIRIHPADDVVIARQQLVGGTLLASEGVTVIGLVPPGHK
                     VAVRAIAAGAPVRRYNQIIGSAKLAIAPGQHVHTHNLEFASFARDYAASTGTHPTAFV
                     DVPATFMGIRRGDGRVATRNYVGVLTSVNCSATVARAIADHFRRDIHPEALARYPNVD
                     GVVALTHGGGCATDSAGAPLQLLRRTLGGYARHANFAAILVVGLGCETNQIAGLMEQE
                     SLGQHNGAGTQFHTFNIQDTGGTAKTVAHGVELVRWLLEEANRVTREPASAAHITVGL
                     QCGGSDGYSGISANPALGAAVDRLVRHGGTAILSETPEIYGGEHLLTRRAVSPAVADK
                     LIQRIRWWERYTARND"
     gene            complement(1057..1923)
                     /locus_tag="VNU48_11500"
     CDS             complement(1057..1923)
                     /locus_tag="VNU48_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWI11945.1"
                     /translation="MKLMRYGAKGAEKPGLLDADGVLRDLSHELVDITASTLTPHGLQ
                     RLRDINPALLPAVADPGRIALPWSGCGKFIAVGLNYADHAAESNLPVPAEPVLFTKTV
                     SCMVGCNDPVVLPQGSVKGDWEVELGIVIGTKARYVSEADALKFVAGYCVVNDVSERE
                     YQIERGGTWDKGKGCDTFGPVGPWMVTADEVPDPQALSMWLDVNGQRMQTGSTRTMIF
                     GVAHLVSYISRFMTLYPGDLITSGTPPGVGMGRKPSPVYLKAGDTMHLGIEGLGEQTQ
                     TVHAWDAALIDD"
     gene            complement(1957..2724)
                     /locus_tag="VNU48_11505"
     CDS             complement(1957..2724)
                     /locus_tag="VNU48_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI11946.1"
                     /translation="MTDRLNGKTAFITAAGQGIGRATAEAFVREGARVIATDINEALL
                     AELAQATGCETRVLDVTDPNAVSAAAQAAGEVQILFNGAGYVHAGTILDCDDKAFEFS
                     FNLNVRAMYRVTRAFLPAMLAGAAQGRRGSIVNVASVAGSIKGAPNRFVYGASKAAVI
                     GMTKSIAADFITRGVRCNAICPGTVESPSLRERMAALAKSTDQTLAQAEAWFVSRQPM
                     GRVGTTAEIAALAVYLGSDESAFTTGTAQVIDGGWSN"
     gene            complement(2721..3473)
                     /locus_tag="VNU48_11510"
     CDS             complement(2721..3473)
                     /locus_tag="VNU48_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWI11947.1"
                     /translation="MPIQTIEPRRLYRQIADQLRGLVERGEYAIGSKLPPERDLALQL
                     GVSRPSVREALIALEVEGLVEVRMGSGVYVLPPERARAREVSAEGPLETTRARQLIES
                     ELAAHAAAHMKKAQIAALRNAVALMQQEVDSGHTATRGDRLFHLRIAEAAENSVLERI
                     VGELFDERHNPLFEQLGSHFETAGSWSAAIAEHQAVIAAIASRSPEAARAAMAAHLAN
                     SHGRFTANITHAAGTDAPPALRRKPRPRSLLT"
BASE COUNT          513 a         1360 c         1359 g          603 t
ORIGIN      
        1 ggtcgttgcg cgcggtgtag cgctcccacc agcggatgcg ctggatcagc ttgtcggcca
       61 ccgcggggct gaccgcgcgc cgcgtcagca gatgctcgcc gccgtagatc tccggcgtct
      121 ccgacaggat cgcggtgccg ccgtggcgca ccagccgatc caccgccgca ccgagcgccg
      181 ggttcgcgct gatgccggag tagccatcac tgccgccgca ctgcagcccg accgtgatgt
      241 gcgcggcgct ggccggttcg cgcgtcacgc ggttcgcttc ttcgagcagc catcgcacca
      301 gttcgacgcc gtgcgcgacg gtcttcgcgg tgccgccggt gtcctggatg ttgaaggtgt
      361 ggaactgcgt gccggcgccg ttgtgttgtc caagactttc ctgctccatc agccccgcga
      421 tctggttcgt ctcgcaaccc agcccgacga ccaggatggc cgcgaagttc gcgtgccgcg
      481 catagccgcc caaggtgcgg cgcagcagct gcagcggcgc gccggcgctg tcggtcgcgc
      541 agccgccgcc gtgcgtcagc gcgaccacgc cgtcgacgtt cgggtacctc gcgagcgcct
      601 cggggtggat atcgcgccgg aagtggtcgg cgatcgcgcg cgcgacggtg gccgagcagt
      661 tcaccgaggt cagcacgccg acgtagttgc gcgtcgcgac gcggccatcg ccgcgccgga
      721 tgcccatgaa cgtggccggc acgtcgacga acgcggtcgg gtgcgtgccg gtgctggccg
      781 cgtagtcgcg cgcgaagctg gcgaactcca ggttgtgcgt gtgcacgtgc tggccgggcg
      841 cgatcgcgag cttcgcgctg ccgatgatct ggttgtagcg ccgcaccggc gcgcccgccg
      901 caatcgcgcg caccgcgacc ttgtgccccg gcggcaccag gccgatcacg gtgacgcctt
      961 cgctcgcgag cagcgtgccg ccgacgagct gctggcgcgc gatcacgaca tcgtcggcgg
     1021 ggtggatgcg gatgacgagc gggtcggtca tgtcgctcaa tcgtcgatca gcgccgcgtc
     1081 ccacgcatgc accgtctgcg tctgctcgcc caggccctcg atgcccaggt gcatcgtgtc
     1141 gccggccttc aggtagaccg ggctcggctt cctgcccatg cccacgccgg gcggcgtgcc
     1201 ggaggtgatc aggtcgccgg ggtacagcgt catgaaccgg ctgatgtagc tcacgagatg
     1261 agccacgccg aagatcatcg tcctggtgct gccggtctgc atgcgctggc cgttcacgtc
     1321 cagccacatc gacagcgctt gcgggtcggg cacctcgtcg gccgtcacca tccacgggcc
     1381 gaccgggccg aaggtgtcgc agcccttgcc cttgtcccag gtgccgccgc gctcgatctg
     1441 gtactcgcgc tccgacacgt cgttgacgac gcagtagccg gcgacgaact tcagcgcatc
     1501 ggcctcgctg acatagcgcg ccttggtgcc gatcacgatg ccgagctcga cctcccagtc
     1561 gcccttgacg gaaccctgcg gcagcacgac cgggtcgttg cagccgacca tgcacgacac
     1621 cgtcttcgtg aacagcaccg gctcggcggg caccggcagg ttggattccg ccgcgtggtc
     1681 cgcgtagttc agcccgacgg cgatgaactt gccgcagccg ctccacggca gcgcgatgcg
     1741 acccgggtcc gcgaccgccg gcagcagcgc cgggttgatg tcgcgcaacc gctgcaggcc
     1801 gtgcggcgtg agcgtgctgg cggtgatgtc gacgagttcg tgggagaggt cgcgcagcac
     1861 gccgtcggcg tcgagcaggc ccggcttctc cgcgcccttg gcgccgtatc gcatcagttt
     1921 catggtcgtg tccttggttg agagtctgca gaggtgctaa ttggaccagc cgccgtcgat
     1981 cacctgcgcc gtgccggtcg tgaacgccga ttcgtcggag ccgaggtaga ccgcgagcgc
     2041 ggcgatctcg gcggtcgtgc ccacgcggcc catcggctgg cgcgacacga accaggcttc
     2101 ggcctgcgcc agcgtctggt cggtggactt cgccaacgcc gccatgcgtt cgcgcagcga
     2161 cggcgactcg accgtgcccg ggcagatcgc gttgcagcgc acgccgcggg tgatgaagtc
     2221 ggccgcgatc gacttggtca tgccgatcac cgcggccttg ctcgcgccgt agacgaagcg
     2281 gttcggcgcg cccttgatgc tgccggccac cgaggcgacg ttgacgatcg atccgcggcg
     2341 gccctgcgcg gcgccggcca gcatcgccgg caggaacgcg cgcgtcacgc ggtacatcgc
     2401 ccgcacattc aggttgaacg agaactcgaa ggccttgtcg tcgcagtcca ggatcgtgcc
     2461 ggcatgcacg tagccggcgc cgttgaacag gatctgcacc tcgccggcgg cctgggctgc
     2521 ggcgctcacc gcgttcgggt cggtcacgtc gagcacccgc gtctcgcagc cggtggcctg
     2581 cgcgagctcg gccagcagcg cctcgttgat gtcggtggcg atcacgcgcg cgccttcgcg
     2641 cacgaaggcc tcggcggtgg cgcggccgat gccctggccg gcggcggtga tgaaggcggt
     2701 cttgccgttg aggcggtcgg tcatgtcaaa aggctcctgg gtcggggctt gcggcgcagt
     2761 gcgggcggcg cgtcggtgcc cgcggcgtgc gtgatgttgg cggtgaagcg gccatgcgag
     2821 ttcgccaggt gcgccgccat cgcggcgcgc gcggcctcgg gcgaacggct cgcgatcgcg
     2881 gcgatcaccg cctgatgctc ggcgatcgcg gcgctccagc tgcccgcggt ctcgaagtgg
     2941 ctgccgagct gctcgaacag cgggttgtgg cgctcgtcga acagctcgcc gacgatgcgc
     3001 tcgagcaccg aattctcggc cgcctcggcg atgcgcaggt ggaacaggcg atcgccgcgc
     3061 gtcgcggtgt ggccgctgtc gacttcctgc tgcatcagcg cgaccgcgtt gcgcagcgcg
     3121 gcgatctgcg ccttcttcat gtgcgcggcg gcatgcgcgg cgagctcgct ctcgatcagc
     3181 tggcgcgcgc gagtcgtctc cagcggccct tcggcgctga cctctcgtgc acgcgcccgt
     3241 tcgggcggca gcacgtagac gcccgagccc atgcgcacct cgaccagccc ctcgacctcg
     3301 agcgcgatca gcgcctcgcg caccgagggc cgcgacacgc cgagctgcag cgcgaggtcg
     3361 cgctcgggcg gcagcttcga gccgatcgcg tattcgccgc gctcgaccag gccgcgcagc
     3421 tgatcggcga tctgacggta caggcggcgc ggttcgatgg tctggatcgg catgggggag
     3481 aaggcgccgg gcagagcgcg cgccgaacgg aagtggccaa gtggcctgac caattgggta
     3541 aagaatagtt gacgcgcgcg gatcgcgtca acatcgggtt tgcccgcggc tgcgatcggc
     3601 gcgcgcggtc gaacatcgcg acacacgccg tggcgccagc ggcgtgcgtg cgcgccccgc
     3661 cacgctgcga ccggtacacc gcaaggactt cagaattcca cccatggaac acaacgagcc
     3721 gccgacgact tcacccccgc cgcgcgcgcc atcggcaccc gccccgcgcg ccccggtgtc
     3781 gcgccgcggc cgctggatcg gcagcatcgt cgcgctgctc gccctcgcgc tgctc
//
LOCUS       DATMJB010000536         2661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_377015, whole genome shotgun sequence.
ACCESSION   DATMJB010000536 DATMJB010000000
VERSION     DATMJB010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377015"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VNU48_11515"
     CDS             complement(<1..1254)
                     /locus_tag="VNU48_11515"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWI11948.1"
                     /translation="MKRFNLSEWAVTHRPMVLFLIIATLIVGVISFSRLGRLEDPNFD
                     VPTMTAIVVWPGATAQDVQDEVLNRIERKLQELDHFDYVRSFARQGYGGITLWMKGGS
                     SKEELENAWYQVRKKIGDVRQEFPEGVRGPFFNDEYNDRYSVLYAVSAPELSMAELLT
                     VAEGVKRRIQAVPGAAKVDVLGKQAERVYVELSTRRLAALGIPATAVFDALKRQNLVA
                     PAGSSDTTQDRVQVRVDGGLHSAADVANVTLEVGGQLLRLADIATVRTGYEDPPSMTI
                     RHNGVPVLAIGVTMQANGNVLDFGHALEERLALARLRQELPAGVEVQQYADQPRVVEE
                     SVWEFERSFLEALAIVLAVSFLFLGWRTGIVVAVSVPLVLGLVATVMYAAGWNLDRIS
                     LGALIIALGLLVDDAMIAVEMMVVKL"
     gene            complement(1266..2402)
                     /locus_tag="VNU48_11520"
     CDS             complement(1266..2402)
                     /locus_tag="VNU48_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWI11949.1"
                     /translation="MNRTFPTVLSHLTMAAAAGALLTACGDPPVPVVKIKPVFVTTVT
                     GAESSQTRSFTAVVRARVETDLGFRAGGKVVDRLVEVGDAVKAGQALARLDPADYQLA
                     VRAAADQVQAASVDAQQAASDEARLRRLLADGSVGSADHERQKARADAAAARLEQARR
                     QLDLARNREGYTTLVAPYAGVVTALRFERGQVVAEGQPVLSVARDGEREIVAELPEEW
                     VGRARTLTATATPWQDTTTSLRLVLRELSPLASAQGRTFRVRYAAAPESRTQVGALPL
                     GSTMQLNLAEKSTGPASVVLPVGALVKASGSAGVWVLDDKGSGLVFKPVQVLNMDDAS
                     VRVTGLAAGSRVVSAGVQKLDAGIKVRAIERLPEAAPAPVAKSS"
     gene            complement(2399..>2661)
                     /locus_tag="VNU48_11525"
     CDS             complement(2399..>2661)
                     /locus_tag="VNU48_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWI11950.1"
                     /translation="ATALASTVDHRRRSLQRAQSLQREGQIDLLVLLDVQRSVLSSEL
                     ALSDSRLQQALADVQLYKALGGGFEPARAGTAMNSLSERTPQ"
BASE COUNT          415 a          963 c          884 g          399 t
ORIGIN      
        1 cagcttcacc accatcatct ccaccgcgat catggcgtcg tcgaccaaca gacccagcgc
       61 gatgatcagt gcgcccaggg agatgcgatc cagattccag ccggccgcgt acatgacggt
      121 cgccaccagc ccgagcacca acggcaccga cacggccacc acgatgccgg tgcgccagcc
      181 caggaacagg aaggacacgg ccagcacgat cgccagcgcc tccaggaacg agcgctcgaa
      241 ctcccagact gactcttcca ccacgcgcgg ctggtcggcg tactgctgga cctccacgcc
      301 cgccggcagt tcctgccgca ggcgcgccag cgccagccgc tcttccagcg cgtgaccgaa
      361 gtccagtacg ttgccgttgg cctgcatggt cacgccgatg gccagcaccg gcacgccgtt
      421 gtgacggatg gtcatgctcg gcggatcttc gtagccggtg cgcacggtcg caatgtcggc
      481 cagccgcagc aactgcccac ccacctccag cgtcacgttg gcaacgtcgg cggcgctgtg
      541 cagcccgccg tcgacgcgca cctgcacgcg gtcctgcgtg gtatcggatg aaccggccgg
      601 ggccaccagg ttctggcgct tcagcgcgtc gaacaccgcc gtcgccggga tgccgagcgc
      661 ggccagccgc cgggtggaca gctccacgta cacgcgctcg gcctgcttgc ccagcacatc
      721 gaccttcgcg gcgccgggca cggcctggat acgccgcttc accccttcgg cgaccgtgag
      781 caattcggcc atcgacagct cgggcgcgct caccgcatag agcacgctgt agcggtcgtt
      841 gtactcgtcg ttgaagaacg gcccgcgcac cccttcgggg aactcctggc gcacgtcgcc
      901 gatcttcttg cgcacctggt accaggcgtt ctccagctcc tccttgctgc tgccgccctt
      961 catccacagc gtgatgccgc cgtagccctg gcgcgcgaag ctgcgcacgt agtcgaagtg
     1021 gtccagctcc tgcagcttgc gctcgatgcg gttgagcacc tcgtcctgca cgtcctgcgc
     1081 ggtggcgccg ggccacacga cgatggccgt catcgtcggc acgtcgaagt tggggtcctc
     1141 caggcggccc agccggctga aggatatgac gccgacgatg agcgtcgcga tgatcaggaa
     1201 cagcaccatc ggccggtgcg tgacggccca ttccgagaga ttgaagcgtt tcatggctgc
     1261 tcttgttagc tgctcttggc gacgggggcc ggcgcggctt cgggcaaacg ttcgatggcc
     1321 cgcaccttga tgccggcatc gagcttctgg acaccggcgc tgaccacccg gctgccggcg
     1381 gccaggccgg tgacgcgcac cgacgcgtcg tccatgttca gcacctggac gggtttgaag
     1441 acaaggcccg accccttgtc gtcgagcacc cagacgcctg ccgatccgct ggccttcacc
     1501 agtgcgccca ccggcagaac cacggacgcc ggcccggtgg acttttcggc caggttcaac
     1561 tgcatggtgc tgccaagggg cagggcaccc acttgggtgc gcgactccgg cgctgcggcg
     1621 tagcgcacgc ggaaggtccg gccctgcgcg ctggccagtg gggacagctc gcgcagcacc
     1681 agccgcagcg acgtcgttgt gtcctgccag ggcgtggccg tggcggtgag cgtgcgcgca
     1741 cggcccaccc actcctccgg gagctcggcg acgatctcac gctcgccgtc gcgggccacc
     1801 gacagcacgg gctggccttc ggccacgacc tggccacgct cgaagcgcag ggcggtgacc
     1861 acgccggcgt agggcgccac cagcgtcgtg tagccttccc gattgcgcgc caagtcgagt
     1921 tgacggcgcg cctgttccag ccgagccgcg gccgcatcgg cgcgcgcctt ctggcgctcg
     1981 tgatcggcac tgcccaccga gccgtcggcc agcaggcggc gcaggcgggc ctcgtcggat
     2041 gcggcctgct gcgcatccac cgacgccgcc tgcacctggt cggcggcggc acgcacggcc
     2101 aactgatagt cggccggatc cagccgcgcc agcgcctggc cggccttcac ggcgtcaccc
     2161 acctcgacca agcggtccac caccttcccg ccagcgcgga aacccaagtc cgtttcgaca
     2221 cgggctcgga cgacggcggt gaacgagcgc gtttgcgagg actcggcccc ggtcacggtg
     2281 gtcacgaaga ccggcttgat cttgacgaca gggacgggag ggtcgccgca agcggtcagc
     2341 agcgcgccgg cggcggcggc catcgtcaga tgcgacaaca cggtgggaaa ggtcctgttc
     2401 actggggcgt cctctcggaa agggaattca tggcagtgcc ggcccgggcc ggctcgaagc
     2461 cgccacccag tgccttgtag agctggacgt cggccagcgc ctgctgcagg cggctgtccg
     2521 acagggcgag ttcgctggac agcaccgagc gctgcacatc gagcagtacc agcaggtcga
     2581 tctgaccttc tcgctgcagt gactgggcgc gctgcaggga acggcggcgg tggtcgacgg
     2641 tgctggccaa ggcggtggcc c
//
LOCUS       DATMJB010000537         2949 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_765380, whole genome shotgun sequence.
ACCESSION   DATMJB010000537 DATMJB010000000
VERSION     DATMJB010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765380"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..554)
                     /locus_tag="VNU48_11530"
     CDS             complement(201..554)
                     /locus_tag="VNU48_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HWI11951.1"
                     /translation="MIANALVGLVAAIHVYIVVLEMFLWDKPFGRRVFGNSAEFAAAS
                     RVLAANQGLYNGFLAAGLVWGLSMGAAGQPVQLFFLGCVIVAGVYGALSVGIKILFVQ
                     AVPAALAVAAILLAR"
     gene            complement(645..>2949)
                     /locus_tag="VNU48_11535"
     CDS             complement(645..>2949)
                     /locus_tag="VNU48_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI11952.1"
                     /translation="SGFDEALYGQVARHPQVAIASPVIEIDTYALDAQGERVALKIVG
                     LDALVAGPLAPALMPRPANDADRFALLAPDAVFLNAQARKRLAGARLRVQTPTGSTSL
                     RVQGEIGAEGAALAVMDIAGAQATFGWLGRIGRIDVRLRAGADRATVLRELALPAGIR
                     AAAPDEAAQRVSNVSRAYRVNLTVLALVALFTGAFLVFSILSLSVAKRQPQLALLGVL
                     GLGGRERLALVLAESALLGAVGSALGLALGTALAALALRLLAGDLGGGYFPGVAPALQ
                     FDAGAALVYGGLGMAAAMVGGWLPARDAQRLAPAQALKGLGASAAPAGPAWLGPALLI
                     AGVLLALLPPVAGLPLAAYLGVACLLFGGIACVPVSVALMLKGIAPPRHALPLLAVER
                     ARHQRHTATIAVAGVVASLSLAIALTVMVASFRASVTQWLDTVLPADLYLRAASSLGA
                     GDVATLPASMPERAAQIDGVQRAQAQRVLLLQLAAARPTVMLIARALPDPAQHLPLVG
                     ELVAAPAGATAVYVSEAMVDLYDARPGTTLALPLTGEPRVFVRGVWRDYARQHGAIAI
                     DAADYRRLTGDTRVNDLALWLRPGADTARLQQTLRALASELGLDGALLEFASAGEIRT
                     TSLHIFDRSFAVTYWLQAVAIAIGLFGIAASFSAQVLARRKEFGLLAHLGLTRTQVLA
                     VVAVEGAVWTAAGALLGLLLGLAVSIVLVKVVNPQSFHWTMDLLLPWARLAALCAAVV
                     VAGTSTAWLAARNAAGRQLALAVKEDW"
BASE COUNT          447 a         1131 c         1060 g          311 t
ORIGIN      
        1 cggcttcgcg gcggccttca ctgcgcgcgc cgtggcctgc accacggcgc gcgtgcccgc
       61 acgcagcgtg gcgcgcgaca gcgccccgag cgcgcgcgac agcgaaccgg tccacgaaga
      121 ctttttgcga atgcgtcgcg ccatgccgac ccgtgatcgc ccgaagcggg ccgagccggg
      181 attctgcagt gagcgcggcg ctagcgtgcg agcaggatcg ccgcgaccgc gagcgccgcc
      241 ggcacggcct gcacgaacag gatcttgatg ccgacgctga gcgccccgta cacgcccgcg
      301 acgatcacgc agccgaggaa gaacagctgc accggctgcc cggccgcgcc catgctcagg
      361 ccccagacca ggccggccgc gaggaagccg ttgtacaggc cctggttcgc cgcgagcacc
      421 ctggaggccg cggcgaactc ggcgctgttg ccgaacacgc gccgcccgaa gggcttgtcc
      481 cagaggaaca tctcgagcac gacgatgtag acgtggatcg cggcgaccag acccaccagc
      541 gcgttggcaa tcatcatcgg atgcaccttt cagcgcggac cttgcgcggg cacggcgatt
      601 ctaggggccg cgttgagcgt gtccgagccg accgccgccg cccttcacca gtcctccttc
      661 accgccagcg ccagctgccg gccggccgcg ttgcgcgccg cgagccaggc cgtcgacgtg
      721 ccggcgacga ccaccgccgc gcacagcgcc gccagccgcg cccacggcag cagcaggtcc
      781 atggtccagt gaaagctctg cgggttcacg accttcacga gcacgatgct gaccgccagc
      841 ccgagcaaga ggccgagcag cgcgccggcg gcggtccaga ccgcgccctc gaccgcgacc
      901 accgcgagca cctgcgtgcg cgtcagcccg aggtgcgcga gcaggccgaa ctccttgcgc
      961 cgcgccagca cctgcgccga gaagctcgcc gcgatgccga acaggccgat cgcgatcgcc
     1021 acggcctgca gccagtaggt caccgcgaag ctgcggtcga agatgtgcag cgaggtggtg
     1081 cggatctcgc cggccgatgc gaattcgagc aacgcgccgt ccaggcccag ctcgctcgcc
     1141 agcgcgcgca gtgtctgctg gaggcgcgcc gtgtcggcgc cgggccgcag ccacagcgcg
     1201 aggtcgttga cgcgggtgtc gccggtgagg cgccggtagt cggccgcgtc gatcgcgatc
     1261 gcgccgtgct ggcgcgcgta gtcgcgccac acgccgcgca cgaacacgcg cggctcgccg
     1321 gtcagcggca gcgccagcgt cgtgcccggg cgtgcgtcgt agaggtcgac catggcctcg
     1381 ctgacataga ccgcggtcgc gccggccggc gcggcaacga gctcgccgac cagcggcagg
     1441 tgctgcgccg gatcgggcag cgcgcgcgcg atcagcatca ccgtcgggcg cgccgcggcc
     1501 agctgcaaca gcaggacgcg ctgcgcctgc gcgcgctgca cgccgtcgat ctgcgcggcg
     1561 cgctcgggca tcgaagccgg cagtgtcgcg acatcgccgg cgcccagcga cgacgcggcg
     1621 cgcaggtaca gatcggccgg cagcacggtg tccagccact gcgtgaccga cgcgcgaaag
     1681 ctcgcgacca tcacggtcag cgcgatcgcc aggctcaggc tcgcgaccac gcccgcgacg
     1741 gcgatcgtcg cggtgtggcg ctgatggcgt gcgcgttcca ccgcgagcaa aggcagcgca
     1801 tggcgcggcg gcgcgatgcc cttcagcatc agcgcgacgc tgaccggcac gcaggcgatg
     1861 ccgccgaaca gcagacacgc gacacccagg taggcggcga gcggcaggcc cgcgaccggc
     1921 ggtagcagcg ccaacagcac gccggcgatc agcaaggccg ggccgagcca ggccggcccg
     1981 gccggcgcgg cgcttgcgcc caggcccttc agtgcctgcg ccggcgcgag ccgctgcgcg
     2041 tcgcgcgccg gcagccagcc gccgaccatc gccgcggcca tgccgaggcc cccgtagacc
     2101 agcgccgcgc ccgcatcgaa ctgcagtgcc ggcgcgacgc cggggaaata gccgccgccg
     2161 agatcgccgg cgagcaggcg cagcgcgagc gcagccagcg ccgtgccgag cgccaggccg
     2221 agcgcgctgc cgaccgcgcc gagcagcgcc gattcggcca gcaccagcgc gagccgctcg
     2281 cgcccgccca ggcccagcac gccgagcaac gcgagctgcg gctggcgctt cgccaccgac
     2341 agcgacagga tcgagaacac caggaacgcg ccggtgaaca gcgccacgag cgcgagcacc
     2401 gtcaggttca cgcggtaggc gcgcgagacg ttcgagacgc gctgcgctgc ttcgtcgggc
     2461 gccgcggcgc ggatccccgc cggcagcgcg agttcgcgca gcaccgtcgc gcgatcggcg
     2521 ccggcgcgca atcgcacatc gatgcggccg atgcggccga gccagccgaa ggtggcttgc
     2581 gcgccggcga tgtccatcac cgcgagcgcc gcgccctcgg cgccgatctc gccctgcacg
     2641 cgcaggctcg tgctgccggt gggcgtctgc acgcgcagcc gcgcgccggc gaggcgcttg
     2701 cgcgcctgcg cgttcaggaa caccgcatcg ggcgcaagca gcgcgaagcg gtcggcgtcg
     2761 ttcgccgggc gcggcatcag cgccggcgcg agcgggccgg cgaccagcgc atcgaggccg
     2821 acgatcttca gcgccacccg ctcgccctgc gcgtcgagcg cgtaggtgtc gatctcgatc
     2881 accgggctcg cgatcgcgac ctgcgggtgc cgcgcgacct gcccgtacag cgcctcgtca
     2941 aagccgctg
//
LOCUS       DATMJB010000538         2030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_784468, whole genome shotgun sequence.
ACCESSION   DATMJB010000538 DATMJB010000000
VERSION     DATMJB010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784468"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="VNU48_11540"
     CDS             complement(<1..220)
                     /locus_tag="VNU48_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11953.1"
                     /translation="MDYLTRWRMAADRHAGEPAPPHDARPRSGQTMSNRTTAVRRRGT
                     RRPQASASIGQPNRSQGMSYIDGFVIAVP"
     gene            complement(333..1109)
                     /locus_tag="VNU48_11545"
     CDS             complement(333..1109)
                     /locus_tag="VNU48_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HWI11954.1"
                     /translation="MIVDDEELARLRLRGLVHDCAEPKALVVGEAANAVQALVWLAEQ
                     RCDLVLLDVQMPGRDGTQLAAELKRLAEPPAVVFVTAHAQHALQAFDLDAVDYLTKPV
                     RRERLHAALQRVAQRLALRHGALPVAVAALPDDVPAIVVTDRGRVLRVPLADVLYFKA
                     ELKYVTLRTAEHTYVLDEALSDLEQRLGERFLRVHRNALVARNAVRALERRAAPTGAI
                     EREDEGGEGWAVRMAPVDEWLAVSRRQVAAVREALVAAGR"
     gene            complement(1211..>2030)
                     /locus_tag="VNU48_11550"
     CDS             complement(1211..>2030)
                     /locus_tag="VNU48_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWI11955.1"
                     /translation="VLLWLLVACAARRPFGALPLPGQWVVATGLGAVCGAGGALLAGL
                     AAGNGFDTPPWLAPALAGAGLAALVFHWLRLRAQALLPADTTARLAELQSRIRPHFLF
                     NTLNTALALVRHDPTRAEELLEDLAELFRVAISENPESVSLAEEVELAQRYLAIEQVR
                     FGSRLQVSWELDPDAGHARVPPLLLQPLVENAVRHGIEPAASGGTIRVRTRVKLGRAL
                     ISIVNSAPAAPSQPGNGIALRNVRERLRLMHDVAAQFDTKREADLFRVQIVVPL"
BASE COUNT          287 a          769 c          725 g          249 t
ORIGIN      
        1 ttggaaccgc gatcacgaaa ccgtcgatgt aggacatgcc ttgactcctg ttgggttgac
       61 cgatcgacgc actcgcctga gggcgcctcg tacctcgacg acgaacggca gtagtgcgat
      121 tcgacattgt ctgcccggag cgcgggcgcg cgtcatgtgg cggcgccggc tcgcccgcgt
      181 gccggtcggc cgccatgcgc cagcgcgtca ggtagtccat cggcgacgcg cccaccaggc
      241 gcttgaacct tcgaacgtcg cacgagaccg gaagcgctcg gacccctgca aatcgtgcgc
      301 agctcatcgt ccacccggcg ccggacaacc attcaccgcc ccgcggccac cagcgcctcg
      361 cgcaccgccg cgacctggcg gcgcgacacc gcgagccatt cgtcgaccgg cgccatgcgc
      421 acggcccagc cctcgccgcc ctcgtcttcg cgctcgatgg cgccggtcgg cgcggcgcgg
      481 cgctccagcg cgcgcacggc gttgcgcgcg accagcgcgt tgcggtgcac gcgcaggaag
      541 cgctcgccca ggcgctgctc caggtcggac agcgcctcgt cgagcacgta ggtgtgttcg
      601 gcggtgcgca gcgtcacgta cttgagctcg gccttgaagt acagcacgtc ggcgagcggc
      661 acccgcagca cgcggccgcg gtcggtgacg acgatggccg gcacgtcgtc gggcagcgca
      721 gccacggcga cgggcagcgc gccgtggcgc agcgcgagcc gctgcgcgac ccgttgcagc
      781 gccgcgtgca ggcgctcgcg ccgcaccggc ttggtcaggt agtcgaccgc gtcgaggtcg
      841 aaggcctgca gcgcgtgctg tgcgtgcgcg gtgacgaaca ccacggccgg cggctcggcc
      901 aggcgcttca gctcggccgc cagctgcgtg ccgtcgcgcc cgggcatctg aacgtcgagc
      961 agcacgagat cgcagcgctg ctcggccagc cacaccagcg cctgcaccgc gttcgccgcc
     1021 tcgccgacga ccagggcctt cggctcggcg cagtcgtgca cgaggccacg caggcgcagc
     1081 cgcgcgagct cttcgtcgtc gacgatcaag acccgaaggg atggagcccc tcgccggccg
     1141 ggtacgtccg gcgatccccc caggggatgc gggccggctt ggggcggccc ggcgctcggc
     1201 ccggccccgc tcacaacggc accacgatct ggacgcgaaa cagatcggct tcgcgcttcg
     1261 tgtcgaactg cgccgccacg tcgtgcatca ggcgcagccg ttcgcgcacg ttgcgcagcg
     1321 cgatgccgtt gccgggctgc gagggcgcgg ccggcgcgct gttgacgatc gagatcagcg
     1381 cgcggccgag cttgacgcgc gtgcgcacgc ggatcgtgcc gccgctggcg gccggctcga
     1441 tgccgtggcg caccgcgttc tcgaccagcg gctgcagcag cagcggcggc acgcgcgcat
     1501 ggcccgcgtc ggggtcgagt tcccagctga cctgcagccg gctgccgaag cgcacctgct
     1561 cgatcgccag gtagcgctgc gcgagctcga cctcctcggc cagcgacacc gattcggggt
     1621 tctccgaaat cgcgacgcgg aacagctcgg ccaggtcctc gagcagctct tcggcgcgcg
     1681 tcgggtcgtg ccgcaccagc gcgagcgcgg tgttcagcgt gttgaacagg aagtgcggcc
     1741 ggatgcgcga ctgcagttcg gcgagccgcg ccgtcgtgtc ggccggcagc agcgcctggg
     1801 cgcgcaggcg cagccaatgg aacaccagcg ccgcgaggcc ggcaccggcc agcgccggcg
     1861 cgagccaggg cggcgtatcg aagccgttgc cggccgccag acccgcgagc agcgcgccgc
     1921 ccgcgccgca gaccgcgccc agccccgtcg cgacgaccca ctgcccgggc agcggcaagg
     1981 caccgaacgg ccgccgcgcg gcgcaggcca ccagcagcca cagcagcacc
//
LOCUS       DATMJB010000539         5651 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_561497, whole genome shotgun sequence.
ACCESSION   DATMJB010000539 DATMJB010000000
VERSION     DATMJB010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5651
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561497"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VNU48_11555"
     CDS             <1..1059
                     /locus_tag="VNU48_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWI11956.1"
                     /translation="DPRALALSLGSVQLLERLGAWRADLAQPILEVHVSQAPPSTTAL
                     LGSWFGEPALRIRAIDEAVPLLGAVLSYGSIVAPLQQVWMAAEAAEPTRLHSRFGQPV
                     AALKNLGDAVEVDAGIVERFDLAIVAEGGVFAEQARKALTHDYQQTAWVGKVTFGSGA
                     EPRAAGVAFERFTRHGPVALLPLKDGGAGLVWCVPSGDDPVRELTDAQRVTVLNSIFH
                     PATGRIASITPLKDFALGLNAERTLTDGRTVRIGNAAQTLHPVAGQGLNLGLRDAFEL
                     VHALARNRDVAAVLRRLEWQRGPDRWAMIAATDFLARSFTWKLPGAGAARGLGLAALQ
                     ALAPLKSALARQMMFGRR"
     gene            complement(1088..2728)
                     /locus_tag="VNU48_11560"
     CDS             complement(1088..2728)
                     /locus_tag="VNU48_11560"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HWI11957.1"
                     /translation="MTSAARTVPTAPIAGQRPGTSGLRKKVAVFRQPGYLENFVQSIF
                     DSLDGFKGQTLVLGGDGRFYNDVAIQTILRSAAANGFGRVLVGRHGILSTPAASCVIR
                     KHRAFGGIILSASHNPGGPDGDFGIKYNTGNGGPAPEKITEAIYANAQKIARYLTVDA
                     GDVNLAVPGETMLAGMVVEVIDPVADHADLLATLFDFDRIGALLRSGTLRMCFDAMHA
                     VTGPYAHEIFVRRLGASADTVMNGVPLPDFGGGHPDPNPTYAEDLIELMFNDGAPDFG
                     AASDGDGDRNMIVGRRFVVTPSDSLAVLAANAHLVPGYAAGLKGVARSMPTSAAVDRV
                     AAKLGIRCFETPTGWKFFGNLLDAGEVTLCGEESAGTGSDHVREKDGVWAVLFWLNIL
                     AVRGVSVEQVLRDHWKTYGRNYYSRHDYEGVASAAANDLIAQLRAALPTLPGRVIEGA
                     TIASADDFAYTDPVDGSVSSAQGIRIGLADGSRIVYRLSGTGTEGATLRVYLERFEPD
                     PARHAVETQQALAGLIVLADTLAGIRARTGRDAPTVIT"
     gene            complement(2913..3956)
                     /locus_tag="VNU48_11565"
     CDS             complement(2913..3956)
                     /locus_tag="VNU48_11565"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWI11958.1"
                     /translation="MTRIIPGRLRPAARLVVAFVLAALVLLTLAQRASPLSPWWLELT
                     RYLPYPMLLAPVAAALLVSCALGWRWVLASAATLALAATLTMGLVWNDSEVGTTSPFR
                     VMTYNIKGAKAAQRAGGLAELAREIARHDPDIVMVQDAQGMSSGPPGPQAPEALLFGG
                     RALHRVGQYVIASRFPLRDCAPGRLPVRGEDVGYLRCVVDVRGVELTLVTVHFESPRS
                     GLNAARREGVDGIAEWRRNYEDRLDQARLLAGTFAAGPRPLVIAGDLNAPQASPVVRA
                     LLATGLRDAFASAGRGYGYSYGQALRVGVSFLRIDHLLVSDGIGVAACEVGDGAPSEH
                     RPVIADLWFRRAP"
     gene            4039..5028
                     /locus_tag="VNU48_11570"
     CDS             4039..5028
                     /locus_tag="VNU48_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HWI11959.1"
                     /translation="MSALVFFKLLAIFVVVAIGWIVGKLRWLVAADSDVDPARILANA
                     AYYIFVPALLFRTTARIAFPTMPWGTLVAVFVPLVLMLLAVYALQRRANRDGRLPVAA
                     ASVRAISAVFGNTVQVGIPMAAALFGETGLAIHLAIVSLHALVLLTVATALVEPDLAR
                     AQQRSGAAPVHLAATLLTTARNTVIHPVVLPVLAGLAWNALGLPLPAVADEILQTLGQ
                     AVVPLCLVLIGMSLAYYGVQGAAKAAVWLSVCKLLVLPALVLAIARWGFQLTGLPLAV
                     VVMMAALPIGSNALIFAQRYRTLEAETTAATVFSTLAFGVVAPAWLALLSRIG"
     gene            complement(5054..>5651)
                     /locus_tag="VNU48_11575"
     CDS             complement(5054..>5651)
                     /locus_tag="VNU48_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI11960.1"
                     /translation="RGATEVRTALLDANDTPRHAEVIDAAFAAFGGFDAVLIAHGVLP
                     DQSACEASAAAALASFDTNARSVLALLTPIANRLERQRSGVIAVLSSPAADRGRSSNY
                     VYGAAKAAVAVFASGLRQRLARSGVRVLTLTPGFVDTPMTAAFPKGALWATPERVAAD
                     IDAALRGGFGTVYTPWFWRWIMLVIRSIPERLFVRMKL"
BASE COUNT          767 a         2020 c         2066 g          798 t
ORIGIN      
        1 gacccgcgtg cgctcgccct gtcgctcggc agcgtgcagc tgctcgagcg cctcggcgcc
       61 tggcgcgccg atctggccca gccgatcctc gaggtccatg tgtcgcaggc gccgccgtcg
      121 acgaccgcgc tgctaggcag ctggttcggc gagccggcgc tgcgcatccg cgcgatcgac
      181 gaggcggtgc cgctgctcgg cgcggtgctg agctacggca gcatcgtcgc gcccttgcag
      241 caggtctgga tggcggccga ggcggccgag ccgacgcgcc tgcacagccg cttcggccag
      301 ccggtggcgg cgctgaagaa tctgggcgat gcggtcgagg tcgatgccgg catcgtcgag
      361 cgcttcgatc tcgcgatcgt cgccgagggc ggagtgttcg ccgagcaggc gcgcaaggcg
      421 ctcactcatg actaccagca gaccgcctgg gtcggcaagg tcacattcgg gtccggtgcc
      481 gaaccgcgcg ccgcaggcgt cgcgttcgaa cgcttcacgc gccacggccc ggtcgcattg
      541 ctgcccttga aagacggcgg cgccgggctg gtgtggtgcg tgcccagcgg cgacgacccg
      601 gtgcgcgagc tgaccgacgc gcagcgcgtg acggtgctca acagcatctt ccacccggcg
      661 accgggcgca tcgcgtcgat cacgccgctg aaggacttcg cgctcgggct gaacgccgag
      721 cgcacgctga ccgacggccg cacggtgcgc atcggcaacg ccgcgcagac cctgcacccg
      781 gtcgccggcc agggcctgaa cctggggctg cgcgatgcgt tcgagctcgt ccatgcgctg
      841 gcgcgcaacc gcgatgtggc cgcggtgctg cgccgcctcg aatggcagcg cggcccggac
      901 cgctgggcga tgatcgccgc gaccgacttc ctcgcgcgca gcttcacctg gaagctgccc
      961 ggcgcgggcg cggcgcgcgg gctcggcctc gcggcgctgc aggcgctggc gccgctgaag
     1021 tcggcgctgg cgcgccagat gatgttcggg cggcgctgag cccgggttcg gacagagcgc
     1081 ctggccctca ggtgatcacc gtgggcgcgt cgcgcccggt gcgcgcgcgg atgccggcga
     1141 gcgtgtcggc caacacgatc agcccggcca gcgcctgctg ggtctcgacc gcgtggcgcg
     1201 ccgggtcggg ctcgaagcgc tcgaggtaga cgcgcaaggt cgcgccctcg gtgccggtgc
     1261 ccgacagccg gtagacgatg cgcgagccgt cggccagccc gatgcggatg ccctgcgcgc
     1321 tgctgaccga gccgtcgacc gggtctgtgt aggcgaagtc gtcggcgctg gcgatcgtcg
     1381 ccccttcgat cacccggccc ggcagtgtcg gcagcgccgc gcgaagctgc gcgatcaggt
     1441 cgttcgccgc ggcgctggct acgccctcgt agtcgtggcg cgagtagtag ttgcgcccgt
     1501 aggtcttcca gtggtcgcgc aacacctgct cgaccgacac gccgcgcacc gcgaggatgt
     1561 tgagccagaa cagcacggcc cagacgccgt ccttctcgcg cacgtggtcg gagccggtgc
     1621 cggcgctctc ctcgccgcac agcgtgactt cgccggcgtc gagcaggttg ccgaagaact
     1681 tccagccggt cggcgtttcg aagcacctga tgccgagctt cgcggcgacg cgatcgaccg
     1741 ccgcactcgt cggcatcgaa cgcgccacac ccttcagccc ggcagcgtag ccgggcacca
     1801 ggtgcgcgtt cgcggccagc accgcgaggc tgtcactggg cgtgacgacg aagcggcggc
     1861 cgacgatcat gttgcggtcg ccgtcgccgt cgctcgcggc gccgaagtcg ggcgcgccgt
     1921 cgttgaacat cagctcgatc aggtcttccg cgtaggtcgg gttcgggtcc gggtggccgc
     1981 cgccgaaatc gggcaagggc acgccgttca tcacggtgtc ggcgctcgcg cccaggcgcc
     2041 tgacgaagat ctcgtgcgcg taggggccgg tgaccgcatg catcgcgtcg aagcacatgc
     2101 gcagcgtgcc cgagcgcagc agcgcgccga tgcgatcgaa gtcgaacagc gtcgcgagca
     2161 ggtcggcatg gtcggcgacc gggtcgatga cctcgaccac catgcccgcc agcatcgtct
     2221 cgcccggcac cgcgaggttc acgtcgcccg cgtccaccgt cagatagcgc gcgatcttct
     2281 gcgcgttcgc gtagatcgcc tcggtgatct tctctggcgc gggcccgccg ttgccggtgt
     2341 tgtacttgat gccgaagtcg ccgtcgggcc cacccgggtt gtggctggcc gacaggatga
     2401 tgccgccgaa cgcgcggtgc ttgcggatca cgcagctcgc cgccggcgtc gacaagatgc
     2461 cgtgccggcc gaccagcacg cggccgaagc cgttcgccgc ggcgctgcgc aggatggtct
     2521 ggatcgccac gtcgttgtag aagcggccat cgccgcccag caccagcgtc tggcctttga
     2581 agccatccag cgaatcgaag atcgactgga cgaagttctc gaggtagccg ggttgccgga
     2641 acaccgcaac cttcttgcgc aggccggagg tgccggggcg ctggccggcg atgggtgcag
     2701 taggaacggt ccgggcggca gatgtcatgc gaactccgtg aagcgtcggc gggtctcggt
     2761 ggccgacggg actcgataat aggcaaacgg cgctgctcga cgtgttccag ggcgaggcga
     2821 ggcgcaccgg cctgctgctc gacgcgaacg tcggcggctc gcccttcgct ggtggaatgc
     2881 gagcgcgcgc gcagattcgc ggcacgcgcc gcctacggcg cgcgccggaa ccacaggtcg
     2941 gcgatgaccg gccgatgctc cgagggcgcg ccgtcgccga cctcgcaggc ggccacgccg
     3001 atgccgtcgc tgacgaggag gtggtcgatg cgcaggaacg acacgccgac gcgcagcgcc
     3061 tggccgtagc tgtagccgta gccgcgcccg gcgctcgcga acgcgtcgcg cagcccggtg
     3121 gccagcagcg cgcgcaccac cggtgaggcc tgcggcgcgt tcaggtcgcc ggcgatcacc
     3181 agcgggcgcg ggcccgcggc aaacgtgccc gcgagcaggc gcgcctggtc gagccggtct
     3241 tcgtagttgc ggcgccattc ggcgatgccg tcgacgcctt cgcggcgcgc cgcgttcagc
     3301 ccgctgcgcg gcgattcgaa atgcaccgtg accagcgtca gctcgacacc gcgcacatcg
     3361 acgacgcagc gcaggtagcc gacgtcctcg ccgcgcaccg gcagccgccc cggcgcgcag
     3421 tcgcgcagcg ggaagcggct cgcgatcacg tactggccga cgcggtgcag cgcgcgcccg
     3481 ccgaacagca gcgcctccgg cgcctgcggg ccgggcgggc cggacgacat gccctgcgcg
     3541 tcctggacca tcacgatgtc gggatcgtgg cgcgcgatct cgcgcgccag ctcggccaac
     3601 ccgccggcgc gctgcgccgc cttcgcgccc ttgatgttgt aggtcatcac gcggaacggg
     3661 ctggtcgtgc cgacctcgct gtcgttccag accagcccca tcgtcagcgt cgcggccagc
     3721 gccagcgtcg ccgcactcgc cagcacccag cgccagccga gcgcacacga caccagcagc
     3781 gccgcggcca ccggcgccag cagcatcggg tagggcaggt agcgcgtcag ctcgagccac
     3841 cacgggctca gcgggctggc gcgttgcgcc agcgtcagca gcacgagcgc ggcgagcacg
     3901 aaggcgacga ccaggcgcgc cgccgggcgc agccggccag ggatgattcg ggtcaaggga
     3961 acaacgcgcg cggtgggtga aggcgggcgc agcttacccg agcgcgccgc gccgaagcct
     4021 tcgcggatac tcgcggccat gtctgcgctg gtgttcttca agctgctcgc gatcttcgtc
     4081 gtcgtcgcga tcggctggat cgtcggcaag ctgcgctggc tggtcgccgc cgacagcgac
     4141 gtcgacccgg cgcgcatcct cgcgaacgcg gcgtactaca tcttcgtgcc ggcgctgctg
     4201 tttcgcacca ccgcgcgcat cgccttcccc accatgccct ggggcacgct ggtcgcggtg
     4261 ttcgtgccgc tcgtgctgat gctgctcgcg gtgtatgcgc tgcagcggcg cgccaaccgc
     4321 gacgggcggc tgccggtcgc ggcggccagc gtgcgcgcga tcagcgcggt gttcggcaac
     4381 accgtgcagg tcggcatccc gatggcggcg gccctgttcg gcgagaccgg gctggcgatc
     4441 cacctggcga tcgtcagcct gcacgcgctg gtgctgctga ccgtggccac cgcgctggtc
     4501 gaacccgacc tcgcgcgtgc ccagcagcgc agcggcgcgg cgcccgtgca cctggccgcg
     4561 acgctgctca cgacggcgcg caacaccgtc atccacccgg tggtgctgcc ggtgctggcc
     4621 ggcctggcgt ggaacgcgct cggcctgccg ctgccggcgg tggccgacga gatcctgcag
     4681 acgctgggcc aggcggtcgt gccgctgtgc ctggtgctga tcggcatgtc gctcgcgtac
     4741 tacggggtgc agggtgcggc gaaggcagcg gtgtggctgt cggtgtgcaa gctgctggtg
     4801 ctgccggcgc tggtgctggc gatcgcccgt tggggctttc agctgacggg cctgccgctc
     4861 gcggtggtcg tgatgatggc cgcgctgccg atcggcagca acgcgctgat cttcgcgcag
     4921 cgctaccgca cgctcgaggc cgagacgacc gcggcgacgg tgttctcgac gctggccttc
     4981 ggggtcgtcg cgccggcctg gctggcgctg ctgagccgca tcggctgacc cgtgctgcgc
     5041 acgacggcgt gcgtcacagc ttcatgcgga cgaacagacg ctccggaatg ctgcggatca
     5101 ccagcatgat ccagcgccag aaccagggcg tgtagacagt gccgaaaccg ccccgcaggg
     5161 cggcatcgat gtcggcggcc acgcgttccg gcgtggccca caacgcgccc ttgggaaagg
     5221 cggcggtcat cggcgtgtcg acgaagccgg gcgtcagcgt cagcacgcgc acgccgctgc
     5281 gagccaggcg ctggcgcaag ccggacgcga acaccgccac cgccgccttg gccgcgccgt
     5341 acacgtagtt gctggagcgg ccgcgatcgg cggccggcga cgacagcacc gcgatgacgc
     5401 ccgagcgctg gcgttcgagg cggttcgcga tcggcgtcag cagcgccagc accgagcgcg
     5461 cgttcgtgtc gaaactggcc aacgccgccg ccgccgacgc ttcgcaggcg gactggtccg
     5521 gcagcacgcc atgcgcgatg agcaccgcgt cgaagccacc gaaggccgcg aacgcggcat
     5581 cgatcacctc ggcgtggcgc ggcgtgtcgt tggcgtcgag cagcgcggtg cggacctcgg
     5641 tcgcgccgcg c
//
LOCUS       DATMJB010000540         3090 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_199598, whole genome shotgun sequence.
ACCESSION   DATMJB010000540 DATMJB010000000
VERSION     DATMJB010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199598"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VNU48_11580"
     CDS             complement(<1..1101)
                     /locus_tag="VNU48_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWI11961.1"
                     /translation="MHPASLAAAPLPSPSSAAPRSDWATLRRLLPYVWQWRWRVLVAL
                     SFLVAAKLANVGVPLVLKQLVDALDLKAGDPRSALAVPVALLIAYGALRLSITAFTEL
                     REYLFYPVAARIARRVSLEAFEHLLALSLRFHLERQTGGVSRDLERGSRSIQSLLNYT
                     IYNILPTLVEITLVISLLSTRFDGWFAAIAFGALAIYIVFTVSVTEWRTHFRRAMNEQ
                     DSKANTRAVDALINYETVKYFSNERFEQARYDENLRRLEKVSIKSQTSLSVLNVGQSL
                     IVASAVTLLVWRATLGVVAGTLSLGDLVLVNALMIQLYIPLNFLGVIYREIKQSMIDM
                     ERMFALLGQNREIADAPGAQSLVHRGGNGPGAA"
     gene            1276..1620
                     /locus_tag="VNU48_11585"
     CDS             1276..1620
                     /locus_tag="VNU48_11585"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWI11962.1"
                     /translation="MPMPADANGNGDIFGGWIMAQVDIAGAVLPARIAKGRIATVAVN
                     EFIFKQAVSVNDLLSFYAKVERIGKTSVTVNVEVYAERNPANLHVVKVTEANLTYVAI
                     DRDGQPRLIPRE"
     gene            complement(1623..3023)
                     /locus_tag="VNU48_11590"
     CDS             complement(1623..3023)
                     /locus_tag="VNU48_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWI11963.1"
                     /translation="MSRLVVVSNRVGDPRKPAAGGLAVALGDALAQSGGLWFGWSGRI
                     AEAGAPGEGELHEQRSGAVKLVTIDLSRADHDTYYTGYSNQALWPALHYRLDLADFGA
                     GFFAGYQRVNRLFARKLATLLRPDDVIWVHDYHLIPLAAELRALGCRQRVGFFLHIPL
                     PPPPILAAIPGHEWLMRSFFAYDLVGLQSRVDADHFARFVQEEAHATALGDGEFEAYG
                     RRVVARAFPIGIDVDEFRRLAGSAESRETETMLRDQYSKRRLLVGVDRLDYSKGLPRR
                     FRAFRELLDSYPENRHSATLIQIASPTRESVHAYEDLRHELEGLAGEINGHYGELDWM
                     PIRYMHRVWARRRLPALYRLARVALVTPLRDGMNLVAKEYVAMQDEADPGVLVLSRFA
                     GAAEQMREALLVNPYDIPATAQAIQRALQMPLDERRARHAALLAQTRAQDVHWWRRSF
                     LDALAAVEPRAREAAA"
BASE COUNT          506 a         1052 c         1100 g          432 t
ORIGIN      
        1 agcggcgccg gggccgttgc caccgcggtg caccagcgac tgcgcgccgg gcgcatcggc
       61 gatctcgcgg ttctggccga gcagcgcgaa catccgctcc atgtcgatca tcgactgctt
      121 gatctcgcgg tagatcacgc cgagaaagtt cagcggaatg tagagctgga tcatcagcgc
      181 gttcaccagc accaggtcgc ccaggctgag cgtgccggcg accacgccca gcgtcgcgcg
      241 ccacaccagc agcgtcaccg cgctggcgac gatcaggctc tgcccgacgt tgagcaccga
      301 cagcgaggtc tgcgacttga tcgagacctt ctccaggcgc cgcaggttct cgtcgtagcg
      361 ggcctgctcg aagcgctcgt tggagaagta cttgacggtc tcgtagttga tcagcgcgtc
      421 gacggcgcgc gtgttggcct tgctgtcctg ctcgttcatc gcgcgccgga agtgggtgcg
      481 ccactcggtc acgctgaccg tgaagacgat gtagatcgcc agcgccccga acgcgatggc
      541 cgcgaaccag ccgtcgaacc tggtcgacag cagactgatc accagcgtga tctcgaccag
      601 cgtcggcagg atgttgtaga tcgtgtagtt cagcagcgac tggatcgagc gcgagccgcg
      661 ctcgaggtcg cgcgagacgc cgccggtctg gcgctccagg tgaaagcgca ggctcaaggc
      721 cagcaggtgc tcgaacgcct cgagcgagac gcgccgcgcg atccgcgccg ccaccgggta
      781 gaacaggtac tcgcgcagct cggtgaacgc ggtgatcgac agccgcagcg cgccgtaggc
      841 gatcagcagc gccaccggca ccgccagcgc gctgcgcggg tcgccggctt tcaggtcgag
      901 cgcgtcgacg agctgcttca gcaccagcgg cacgccgacg ttggccagct tcgcggcgac
      961 caggaagctc agcgccacca gcacgcgcca gcgccattgc cagacatagg gcagcaggcg
     1021 ccgcagcgtc gcccagtcgg agcgcggtgc agcggacgaa gggctcggca acggcgcagc
     1081 ggcgagcgag gcggggtgca tcagggcggc gaggaacgga aaggtgaaag aatcgcgggc
     1141 aatcgaacag cagttcaagc aggcaggtga agcaatatgg cagagatttc aagtgacggt
     1201 gcggcgcgcg ttgcgccgcc gagttcgatc gcagcgccgc gcctgcccga cgaccgccag
     1261 ctcgtgctgc gcgtgatgcc gatgccggcc gacgcgaacg gcaacggcga catcttcggc
     1321 ggctggatca tggcccaggt cgacatcgcc ggcgcggtgc tgccggcgcg catcgcgaaa
     1381 ggccgcatcg ccaccgtggc ggtgaacgaa ttcatcttca agcaggcggt gtccgtcaac
     1441 gacctgctga gcttctatgc caaggtcgag cgcatcggca agacctcggt caccgtcaac
     1501 gtcgaggtct atgccgagcg caacccggcg aacctgcacg tcgtcaaggt gaccgaggcg
     1561 aacctgacct atgtcgcgat cgaccgcgac ggccagccgc gcctgattcc cagggaatag
     1621 cgctacgccg cggcctcgcg cgcacgcggc tcgaccgccg ccagcgcatc gaggaagctg
     1681 cggcgccacc agtgtacgtc ctgcgcgcgg gtctgcgcga gcagcgcggc gtggcgcgcg
     1741 cggcgctcgt ccagcggcat ctgcagcgcg cgctggatcg cctgcgccgt ggccgggatg
     1801 tcgtacgggt tcaccagcag cgcctcgcgc atctgctcgg ccgcgccggc gaagcgcgac
     1861 agcaccagca cgccggggtc ggcctcgtcc tgcatcgcga cgtactcctt ggcgaccagg
     1921 ttcatgccgt cgcgcagcgg cgtgaccagc gcgacgcggg ccagccggta cagggccggc
     1981 aggcggcgcc gcgcccagac ccgatgcatg tagcggatcg gcatccagtc gagttcgccg
     2041 tagtggccgt tgatctcgcc ggccaggccc tcgagctcgt ggcgcaggtc ttcgtaggcg
     2101 tgcacgctct cgcgcgtcgg cgaggcgatc tggatcagcg tcgcgctgtg gcggttctcg
     2161 gggtagctgt cgagcagctc gcgaaacgcg cggaagcgtc gcggcagccc cttcgaatag
     2221 tccagccggt cgacgccgac cagcagccgg cgcttgctgt actggtcgcg cagcatcgtc
     2281 tcggtctcgc gcgattcggc gctgccggcc aggcgccgga actcgtcgac gtcgatgccg
     2341 atcgggaacg cacgcgcgac cacgcgccgg ccataggcct cgaactcgcc gtcgccgagt
     2401 gcggtggcgt gcgcctcttc ctggacgaag cgcgcgaagt ggtcggcgtc gacgcgcgac
     2461 tgcagcccga ccaggtcgta ggcgaagaac gaacgcatca gccattcgtg gcccgggatc
     2521 gcagccagga tcggcggcgg cggcagcggg atgtgcagga agaagccgac gcgctgccgg
     2581 catccgagcg cgcgcaactc ggcggcgagc gggatcaggt gatagtcgtg gacccagatc
     2641 acgtcgtcgg gccgcagcag cgtggccagc ttgcgcgcga acaggcgatt cacgcgctgg
     2701 tagccggcga agaagccggc gccgaagtcc gcgaggtcga gccggtagtg cagcgccggc
     2761 cacagcgcct ggttcgagta gccggtgtag taggtgtcgt gatcggcgcg cgacaggtcg
     2821 atcgtgacca gcttgaccgc gcccgagcgc tgctcgtgca gctcgccctc gccgggcgcc
     2881 ccggcctcgg cgatcctgcc gctccagccg aaccacaggc cgccgctttg cgcgagcgcg
     2941 tcgcccagcg cgaccgcaag gccgccggcg gccggcttgc gcgggtcgcc gacgcggttc
     3001 gagacgacga cgaggcggct caatggagcc cctcgtccga tgtatacatc ggccgatccc
     3061 ccgcggggat gcgggccggc ttgggagcgg
//
LOCUS       DATMJB010000541         2132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_369007, whole genome shotgun sequence.
ACCESSION   DATMJB010000541 DATMJB010000000
VERSION     DATMJB010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369007"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VNU48_11595"
     CDS             <1..283
                     /locus_tag="VNU48_11595"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWI11964.1"
                     /translation="DPGIGFGKSVAHNLELLRRQRELLTLGWPLLAGWSRKSTLGTIT
                     GREVGQRQAASVAAALAAVQLGARIVRVHDVGATVDALKVWQAAGLLHG"
     gene            328..1656
                     /gene="glmM"
                     /locus_tag="VNU48_11600"
     CDS             328..1656
                     /gene="glmM"
                     /locus_tag="VNU48_11600"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWI11965.1"
                     /translation="MSRAHFGTDGIRGTVGQAPITADFMLRLGHAVGRVLKATEARPT
                     VLIGKDTRISGYMIESALEAGFASAGVDVLLTGPLPTPGVAYLTRALRLSLGVVISAS
                     HNPFGDNGIKFFSARGEKLPDAWELQVEAALEQPPQWVDSARLGKARRIDDAGGRYTE
                     FCKSTFGNELSLKGLKIVVDAAHGAAYSVAPNVFHELGAEVVAIGCAPNGININAGVG
                     ATAPAALVAAVKANAADYGIALDGDADRLQLVDATGRLYNGDELLYLMVADRLAQGLP
                     VPGAVGTLMTNMAVEVALRERGIAFVRAKVGDRYVLEELIARGWQLGGEGSGHLLALD
                     KHTTGDGIVSSLLVLQAVARSGRTLAQLLEGVSLFPQTLINVRLAPGSDWVGNGRLAE
                     VQAEVGRELGDRGRVLIRPSGTEPLVRVMVEARDAAQAQQCAERLAQTLR"
     gene            1846..>2132
                     /locus_tag="VNU48_11605"
     CDS             1846..>2132
                     /locus_tag="VNU48_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWI11966.1"
                     /translation="MIVAAFATAGSATFAQEITGAGATFPAPIYAKWADAYNKATGVR
                     VNYQSVGSGAGIQQIRAKTVDFGASDMPLKDEDLAKDGLMQFPTVIGGVVPV"
BASE COUNT          324 a          719 c          767 g          322 t
ORIGIN      
        1 cgacccgggc atcggcttcg gcaagagcgt ggcccacaat ctcgagttgc tgcggcgtca
       61 gcgcgagttg ctgacgcttg ggtggccgct gctggccggc tggtcgcgca agtccacgct
      121 cggcacgatc accgggcgcg aggtcgggca gcgccaggcc gccagcgtgg ccgccgcgct
      181 cgcggccgtg caattgggcg cgcgcatcgt gcgcgtgcac gatgtcggcg cgacggtgga
      241 cgccctgaag gtctggcagg cggcgggcct gctgcacgga taatccgtcg gcctgccggg
      301 aaggcgttgg acaaccagga acgagacatg agcagagcgc atttcggcac cgacggcatt
      361 cgcggcacgg tgggccaggc gccgatcacc gccgatttca tgctgcgcct gggccacgcc
      421 gtcggccgcg tgctgaaggc gaccgaagcg cgcccgacgg tactgatcgg caaggacaca
      481 cgcatctccg gctacatgat cgaatcggcg ctcgaggccg gcttcgcgtc ggcgggcgtc
      541 gacgtgctgc tcaccgggcc cctgccgacg cccggcgtgg cctacctgac gcgcgcgctg
      601 cgcctgagcc tgggcgtggt gatcagtgcc tcgcacaacc cgttcggcga caacggcatc
      661 aagttcttct cggcgcgcgg cgagaagctg cccgatgcct gggagctgca ggtcgaggcc
      721 gcgctcgaac agccgccgca gtgggtcgat tcggcacgcc tgggcaaggc gcgtcgcatc
      781 gacgacgccg gcgggcgcta caccgagttc tgcaagagca ccttcggcaa cgagctgtcg
      841 ctcaaaggcc tgaagatcgt cgtcgatgcg gcccacggcg ccgcctacag cgtcgcgccc
      901 aatgtgttcc acgagctcgg ggccgaggtc gtcgcgatcg gctgcgcgcc gaacggcatc
      961 aacatcaacg ccggcgtcgg cgccacggcg ccggcggcgc tggtcgcagc ggtgaaggcg
     1021 aacgccgccg actacggcat cgcgctcgac ggcgatgccg accgcctgca attggtggat
     1081 gccaccgggc ggctgtacaa cggcgacgag ctgctctacc tgatggtcgc ggatcgcctc
     1141 gcgcagggcc tgccggtgcc gggcgcggtc ggcaccttga tgaccaacat ggcggtcgag
     1201 gtcgcgctgc gcgagcgcgg catcgccttc gtgcgcgcca aggtcggcga ccgttacgtg
     1261 ctcgaggaac tgatcgcacg cggctggcag ctcggcggcg agggctccgg ccacctgctg
     1321 gcgctggaca agcacacgac cggcgacggc atcgtcagca gcctgctggt gctgcaggcg
     1381 gtcgcgcgca gcggccgcac gctggcacag ttgctcgaag gcgtgagcct gttcccgcag
     1441 accttgatca atgtgcggct ggcgcccggc agcgactggg tcggcaacgg gcgtctcgcc
     1501 gaggtgcagg ccgaggtcgg gcgcgagctc ggtgatcggg gccgggtgct gatccgcccg
     1561 tccggcaccg agccgctggt gcgtgtgatg gtcgaggcgc gcgatgccgc gcaggcgcag
     1621 cagtgcgccg aacgcctggc gcagaccctg cgctgagcag cgcgctcgac gcgactctgc
     1681 gtgcgcttcg tgacagggcc gtgaaatgcg tgtgacagac gacgggtgcc gtcttcgcgc
     1741 tgtcacagtc gtgtcacacg gcgtccctag agtggcgcgc attcagatga tgttccccac
     1801 ccacagaaag gtgtgcccgt gaagctctcc aatattcgtt ccgccatgat cgtcgccgcg
     1861 ttcgctaccg ccggttccgc aacgttcgcg caggaaatca ccggagccgg cgcgacgttc
     1921 ccggctccga tctatgccaa gtgggcggat gcctacaaca aggcgaccgg tgttcgcgtc
     1981 aactaccagt cggtgggctc gggtgccggc atccagcaga tccgtgccaa gaccgtggac
     2041 ttcggcgcgt ccgacatgcc gctcaaggac gaggacctcg ccaaggacgg cctgatgcag
     2101 ttcccgacgg tgatcggcgg cgtcgtgccg gt
//
LOCUS       DATMJB010000542         4535 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_733002, whole genome shotgun sequence.
ACCESSION   DATMJB010000542 DATMJB010000000
VERSION     DATMJB010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733002"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..956
                     /locus_tag="VNU48_11610"
     CDS             3..956
                     /locus_tag="VNU48_11610"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HWI11967.1"
                     /translation="MGLSMLVYVISDGYDLGVGMLMHRASDAEKDVMIASIGPFWDAN
                     ETWLVLGVGILLIAFPRAHGVVLGELYLPVALMLIGLTLRGVAFDFRVKAKDTHKRTW
                     DRLFFAGSTLAAVSQGWMLGRYVSGFGSGWNYPLFAAAIALALPMAYVLMGATWLVMK
                     TDGQLQERAVHWARVAWAPMVGGMVLISMATPWISQIVRERWFELPEVIALMAIPLMT
                     GVALVAVRGVLRSPAVLGALCWLPFVLLIIVFVLGFLGLAYSIYPYVVIGQLTIWQAA
                     SSPEALKVILIGVCISVPAIAGYTIFSYRVFRGKTGALSYA"
     gene            complement(1027..1755)
                     /locus_tag="VNU48_11615"
     CDS             complement(1027..1755)
                     /locus_tag="VNU48_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HWI11968.1"
                     /translation="MTSLLRSTIALALAATFAHSAFAQEALIRKNLAERLPDFPKIDE
                     VNKTAIPGLYEVRIGTEIVYTDEHGDYVVQGNIIDTKTRRDMTQARIDKLTAIDFAAL
                     PLKDAIVWKQGTGERKLVVFADPNCGYCKKFETEMQQVKDVTVYTFLIPILGGDSPDK
                     ARNAWCAKDNATAWRDWMIKGAVLPRSMGSCDTSALQRNLAMAKKYRVTGTPALVFED
                     GKRVPGALPPAEVEKQLVASRGKA"
     gene            complement(1765..2877)
                     /locus_tag="VNU48_11620"
     CDS             complement(1765..2877)
                     /locus_tag="VNU48_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWI11969.1"
                     /translation="MKKDFDVQVLGAGIVGRCLALALARAGLSVALRPEGARPADSDD
                     VRAYALNAASVELLRSLKVWDALPAHAATAVLDMHVQGDAHASVIDFSAWQQHVSALA
                     WITDAALLERELAAAVRFAPHITVVDADVRAPLTALCEGKASTTRAALGVAFERVAYG
                     HQAIAARLTSSQAHQGIARQWFRAPDVLALLPFDAPQPQRSCALVWSLPNERAQWLLA
                     LDPPAFEHELMHASGGEAGTLTLASERAAWPLMQANASAWCGPGWVLLGDAAHVVHPL
                     AGQGLNLGLADVAALVEVIRQREPWRALGDAKLLRRYARERVAKTWAMGQLTDGLLRL
                     FADDLPLARELRNRGLTLVNHLSPLKRWLTARALNS"
     gene            2970..3848
                     /locus_tag="VNU48_11625"
     CDS             2970..3848
                     /locus_tag="VNU48_11625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HWI11970.1"
                     /translation="MNSIAVPSEGDQAVSIASLHVYPIKSCGGVDLDEALLIETGLEF
                     DRAWMLVDPAGRFVTQRELPRMALVRPVLKSSEMVLRAPGMLALHVALDRVETATRAT
                     VWDDEVAAFDMGELCAQWFTDLLGRPLRLVRFDPAQKRLSERRWTGALDAENGFADAF
                     PLLVTARASITELNRRLEANGGAAVTMARFRPNLVLDGLEAHGEDALDEIVFATDDGP
                     VRLKLVKPCARCTIPDVDPVTAATGHAVSDALAGYRADARVGGQITFGMNAVIVEGLE
                     RALRVGMTGHATFKFD"
     gene            complement(3835..>4535)
                     /locus_tag="VNU48_11630"
     CDS             complement(3835..>4535)
                     /locus_tag="VNU48_11630"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWI11971.1"
                     /translation="LFRDDDGVARCQWCQATPLYRQYHDTEWGFPVTDDRRLFEKLCL
                     EGFQAGLSWLTILNKRESFRAGFANFDAEHVARFKEADITRLLADAGIVRHRGKIEST
                     INNAKRVIELRAEFGSLAAWAWSHEPSPRSRPKKMTLAALRANTTSPESVAMSKDLKK
                     RGWSFVGPTTVYAFMQAMGLVNDHLEGCHVRARALAAPRPNSGQKIDDAEQAEQRAAD
                     EVRPQFRMPCINRT"
BASE COUNT          617 a         1566 c         1605 g          747 t
ORIGIN      
        1 tgatgggcct gtcgatgctg gtctacgtga tcagcgacgg ctacgacctg ggcgtgggca
       61 tgctgatgca ccgcgccagc gatgccgaga aggacgtgat gatcgcctcg atcggcccgt
      121 tctgggacgc caacgagacc tggctggtgc tgggcgtggg gatcctgctg atcgcgttcc
      181 cgcgcgccca cggcgtggtg ctcggtgagc tgtacctgcc ggtggcgttg atgctgatcg
      241 ggctgacgct gcgcggcgtc gcgttcgact tccgcgtgaa ggccaaggac acccacaaac
      301 gcacctggga ccggctgttc ttcgccggct cgacgctggc cgcggtgtcg cagggctgga
      361 tgctggggcg ctatgtcagc ggcttcggca gcggctggaa ctacccgctg ttcgcggcgg
      421 cgatcgcgct ggcgctgccg atggcctatg tgctgatggg ggcgacctgg ctggtcatga
      481 agaccgacgg gcagttgcag gagcgtgccg tgcactgggc ccgcgtggcc tgggcgccga
      541 tggtgggcgg catggtgctg atctcgatgg ccacgccctg gatcagccag atcgtgcgcg
      601 agcgctggtt cgagttgccc gaggtgatcg cgctgatggc gatcccgctg atgaccggcg
      661 tggcgctggt cgcggtgcgc ggtgtgctgc gttcgccggc ggtgctcggg gccctgtgct
      721 ggctgccctt cgtgctgctg atcatcgtgt tcgtgctcgg cttcctgggc ctggcgtaca
      781 gcatctatcc ctacgtcgtg atcggacagc tgacgatctg gcaggccgcg agcagccccg
      841 aggcgctcaa ggtgatcctg atcggcgtgt gcatctcggt gccggcgatc gccggctaca
      901 cgatcttctc gtaccgcgtg ttccggggca agaccggggc cttgagctac gcgtgatcgg
      961 tacagccggc ggccccaagc aaggcccgac ccggtggcga cggtaccccg tcgccgggcg
     1021 ctccgttcag gccttgccgc ggctcgcgac gagctgcttc tcgacctcgg ccggcggcaa
     1081 cgcgcccggc acgcgcttgc cgtcctcgaa gaccagcgcc ggcgtgccgg tcacgcggta
     1141 cttcttcgcc atcgcgagat tgcgctgcag cgccgacgtg tcgcagctgc ccatgctgcg
     1201 cggcagcacg gcgcccttga tcatccagtc gcgccaggcc gtggcgttgt ctttcgcgca
     1261 ccaggcgttg cgcgccttgt cgggcgagtc gccgcccagg atcgggatca ggaaggtgta
     1321 gaccgtcacg tccttgacct gctgcatctc ggtctcgaac ttcttgcagt agccgcagtt
     1381 cgggtcggcg aacaccacca gcttgcgctc gccggtgccc tgcttccaga cgatcgcgtc
     1441 cttcagcggc agcgcggcga agtcgatcgc ggtgagcttg tcgatgcgcg cctgcgtcat
     1501 gtcgcggcgc gtcttcgtgt cgatgatgtt gccctggacc acgtagtcgc cgtgctcgtc
     1561 ggtgtagacg atctcggtgc cgatgcgcac ctcgtacagc ccggggatcg cggtcttgtt
     1621 gacctcgtcg atcttcggga agtcgggcag ccgctcggcc aggttcttgc ggatcagcgc
     1681 ttcctgcgcg aacgccgagt gggcaaacgt cgccgccaag gccagcgcaa tcgtcgagcg
     1741 aagcagagat gtcatgtcgg tctttcagga gttcagcgcc cgcgccgtca gccagcgctt
     1801 cagcggggac aggtggttca ccagagtcag gccgcggttg cgaagttccc gcgccagcgg
     1861 cagatcgtcg gcaaaaagtc gcagcaggcc gtcggtcagc tggcccatcg cccaggtctt
     1921 tgccacgcgc tcgcgggcgt agcggcgcag cagtttcgcg tcgccgagcg cgcgccaggg
     1981 ctcgcgctgg cggatcacct cgaccagcgc ggccacgtcg gccagcccca ggttcaggcc
     2041 ctggccggcc agcggatgca cgacatgcgc ggcgtcgccc agcagcaccc agcccgggcc
     2101 gcaccaggcg ctggcgttcg cctgcatcag cggccatgcg gcacgctccg aggccagcgt
     2161 cagcgtgccg gcctcgccgc cgctggcgtg catcagctcg tgctcgaacg ccggcgggtc
     2221 gagcgcgagc agccactgcg cgcgctcgtt cggcagcgac cagaccagcg cgcaggagcg
     2281 ctgcggctgc ggcgcatcga agggcagcag cgccagcacg tcgggcgcgc gaaaccattg
     2341 ccgcgcgatg ccctggtggg cctgcgaact ggtcagtcgc gcggcgatcg cctggtggcc
     2401 gtaggctacg cgctcgaacg cgacgcccag cgccgcgcgc gtcgtcgacg ccttgccctc
     2461 gcacagcgcg gtcagcgggg cccgcacgtc ggcgtcgacc acggtgatgt gcggcgcgaa
     2521 gcgcaccgcc gcggcgagct cgcgctcgag cagcgcggcg tcggtgatcc aggccagcgc
     2581 gctgacgtgc tgctgccagg ccgagaagtc gatgaccgag gcatgggcgt cgccctgcac
     2641 gtgcatgtcg agcacggccg tcgcggcgtg cgcgggcagc gcgtcccaga ccttcaggct
     2701 gcgcagcagc tcgaccgagg cggcgttcag tgcgtaggcg cgcacgtcgt cgctgtcggc
     2761 cgggcgcgcg ccttccggcc gcagcgcgac gctcagcccg gcgcgcgcca gcgccagcgc
     2821 gaggcagcgc ccgacgatgc ccgcaccgag cacctgcaca tcgaagtcct tcttcatcgg
     2881 ggtcgattgt agggaacggc ccggcgcggc gccccccccg gggtcaaggg cacaatcgcc
     2941 tggctttttc aacgcgcagg aaccggccca tgaacagcat cgcagtgccc tcggagggcg
     3001 atcaggcggt cagcatcgcg tcgctgcacg tctacccgat caagtcctgc ggcggcgtcg
     3061 atctggacga ggccttgctg atcgagaccg gcctcgaatt cgaccgcgcc tggatgctgg
     3121 tcgacccggc cggccgcttc gtcacgcagc gcgagctgcc gcgcatggcg ctggtcaggc
     3181 cggtgctcaa gagctcggag atggtgctgc gcgcgccggg catgctggcg ctgcacgtcg
     3241 cgctcgaccg cgtcgagacg gcgacacgcg cgaccgtctg ggacgacgag gtcgcggcct
     3301 tcgacatggg cgagctgtgc gcgcagtggt tcaccgatct gctcggccgc ccgctgcggc
     3361 tggtgcgctt cgacccggcg cagaagcgcc tgtccgagcg ccgctggacc ggcgcgctcg
     3421 acgccgagaa cggcttcgcc gacgccttcc cgctgctcgt gactgcgcgc gcgtcgatca
     3481 ccgagctgaa ccggcgtctc gaagcaaatg gcggggccgc ggtgacgatg gcgcgctttc
     3541 gtcccaacct cgtgctcgac ggcctggaag cgcacggcga agacgcgctc gacgagatcg
     3601 tgttcgccac cgacgacggc ccggtgcgcc tgaagctcgt caagccttgc gcgcgctgca
     3661 cgattcccga cgtcgacccc gtgaccgccg ccaccggcca tgcggtgagc gacgcgctcg
     3721 ccggctaccg cgccgatgcg cgcgtcggcg gtcagatcac cttcggcatg aacgcggtga
     3781 tcgtcgaggg cctcgagcgc gcgctgcgcg tgggcatgac gggccacgcc acgttcaagt
     3841 tcgattgatg cagggcattc ggaactgcgg ccgcacttcg tcggcggcgc gctgctcggc
     3901 ctgctcggcg tcgtcgatct tctggcccga gttcgggcgc ggcgccgcca gcgcgcgcgc
     3961 ccgcacgtgg cagccttcga ggtggtcgtt gaccagcccc atcgcctgca tgaacgcgta
     4021 aaccgtcgtc gggccgacga agctccagcc gcgcttcttc aggtctttcg acatcgcgac
     4081 cgattcgggc gaggtcgtgt tcgcgcgcaa cgccgccagc gtcatcttct tcggtcgcga
     4141 gcgcggcgac ggttcgtgcg accacgccca ggccgcgagc gagccgaact cggcgcgcag
     4201 ctcgatcacg cgcttcgcgt tgttgatcgt cgactcgatc ttgccgcgat gccgcacgat
     4261 gccggcgtcg gcaagcagcc gcgtgatgtc ggcctccttg aaacgcgcga cgtgttcggc
     4321 gtcgaagttc gcgaagccgg cgcgaaacga ctcgcgcttg ttcaggatgg tcagccagct
     4381 caggccggcc tggaacccct cgaggcagag cttttcgaac aggcgccggt cgtcggtcac
     4441 cgggaatccc cattcggtgt cgtgatactg ccggtagagc ggcgtcgcct ggcaccactg
     4501 gcagcgcgcc acgccgtcgt cgtcgcgaaa gagtc
//
LOCUS       DATMJB010000543         2144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_822206, whole genome shotgun sequence.
ACCESSION   DATMJB010000543 DATMJB010000000
VERSION     DATMJB010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822206"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VNU48_11635"
     CDS             complement(<1..197)
                     /locus_tag="VNU48_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11972.1"
                     /translation="MLPAASPTGAATGGAGRSRRGASKAVLRPVAGAGPRDGGALRSR
                     EDTGLDAGLGGACLRGGASKAP"
     gene            196..930
                     /locus_tag="VNU48_11640"
     CDS             196..930
                     /locus_tag="VNU48_11640"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWI11973.1"
                     /translation="MRLSKRMSELGIASRREADEWIAKGYVRVDDQVVSELGSRVQPH
                     QRITIDAKAKFAQAQRVTVLVNKPVGYVSGQAEDGYEPAVVLVTPANQWREDRSGTRF
                     LREHLKSLVPAGRLDIDSVGLLVLTQDGRIAKQLIGEDSEIEKEYLVRVAMSGGQRLP
                     GSDLVLLRHGLELDGEALRPAQVEWVNEDQLRFVLTQGKKRQIRRMCEAVGLQVLGLK
                     RVRIGKVMLGDLPAGQWRYLAAHETF"
     gene            976..1947
                     /gene="miaA"
                     /locus_tag="VNU48_11645"
     CDS             976..1947
                     /gene="miaA"
                     /locus_tag="VNU48_11645"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HWI11974.1"
                     /translation="MPLATPAHDLRTACLCLAGPTASGKTAAALAIAAAWAGTRPVEI
                     VSVDSALVYRGMDIGTAKPSPEERAFVPHHLIDIIEPSEAYSAARFVVDARRLIGEIR
                     ARGHLPLLVGGTMLYFKALFEGLDAMPAADRGVRAALAAQLERDGLHALYRELQRVDP
                     VSAARLKPADQQRIQRALEVFRVSGQPLSSFHTDKPALALPPLISLEPDDRAWLHRRI
                     EARFHQMLDAGLVDEVRRLGERGDLSADLPSMRSVGYRQTWEALVEGDLSDLPERGVA
                     ASRQLAKRQITWLRGMPQRHRVACDRDDAAAAVVALATTLAAELPRG"
BASE COUNT          287 a          779 c          773 g          305 t
ORIGIN      
        1 ggagccttgg atgcgccgcc gcgcaagcat gcgccgccca agcccgcatc caggcccgtg
       61 tcctcgcgcg agcgcagcgc gccgccgtcg cgcggccccg cgccggcgac cgggcgcaac
      121 accgccttcg atgcgccccg ccgcgatcgt cccgctccgc ccgtcgccgc cccggtcggc
      181 gatgcggccg gcagcatgcg cctgtccaag cgcatgagcg agctcggcat cgcgtcgcgg
      241 cgcgaggccg acgagtggat cgcgaagggc tatgtgcgcg tcgacgatca ggtcgtcagc
      301 gagctcggct cgcgcgtgca gccccaccag cgcatcacga tcgatgcgaa ggccaagttc
      361 gcgcaggcgc agcgcgtcac ggtgctggtc aacaagccgg tgggttatgt ctcaggccag
      421 gccgaggacg gctacgagcc cgcggtcgtg ctcgtcacgc cggcgaacca gtggcgcgag
      481 gaccgctcgg gcacgcgctt cctgcgcgag cacctgaaga gcctggtgcc ggccggacgc
      541 ctggacatcg attcggtcgg cctgctggtg ctgacgcagg acggtcgcat cgcgaagcag
      601 ctgatcggcg aagacagcga gatcgagaag gaatacctcg tgcgggtggc gatgtccggc
      661 ggccagcgac tgcccggatc ggacctggtg ctgctgcgtc acggcctcga gctcgacggc
      721 gaggcgctgc gcccggcgca ggtcgagtgg gtcaacgagg accagctgcg cttcgtgctg
      781 acgcagggca agaagcgcca gatccggcgc atgtgcgaag ccgtcgggct gcaggtgctg
      841 gggctgaagc gcgtgcgcat cggcaaggtc atgctcggcg acctgcccgc cggccagtgg
      901 cgctacctgg ccgcgcacga gacgttctga agggccgtcg gccttcgcgg gccgcgcccc
      961 tgctaacctc gaccgatgcc gcttgccaca cccgcgcacg atctgcgcac cgcctgcctg
     1021 tgcctcgcgg gcccgaccgc atcgggcaag accgcggccg cgctggcgat cgcggccgcg
     1081 tgggccggca cgcggccggt cgagatcgtc agcgtcgact cggcgctggt ctaccgcggc
     1141 atggacatcg gcaccgcgaa gccgagcccg gaagaacgcg ccttcgtgcc gcaccatctg
     1201 atcgacatca tcgagccgag cgaggcctac tcggcggcgc gcttcgtcgt cgacgcgcgc
     1261 cgcctgatcg gcgagatccg cgcgcgcgga catctgccgc tgctggtcgg cggcacgatg
     1321 ctgtacttca aggccttgtt cgagggcctg gacgcgatgc ccgcggccga tcgcggcgtg
     1381 cgcgcggcgc tcgcggcgca gctcgagcgc gacggcctgc atgcgctgta ccgcgagctg
     1441 cagcgcgtcg acccggtcag cgcggcgcgg ctgaagccgg ccgaccagca gcgcatccag
     1501 cgcgcgctcg aggtgttccg cgtcagcggc cagccgctgt cgtcgttcca caccgacaag
     1561 ccggcgctcg cgctgccgcc gctgatctcg ctcgagcccg acgaccgcgc ctggctgcac
     1621 cggcgcatcg aggcgcgctt tcaccagatg ctggacgccg gtctggtcga cgaggtgcgg
     1681 cgcctgggcg agcgcggtga cctgagcgcc gacctgccct cgatgcgcag cgtcggctac
     1741 cgccagacct gggaggcgct ggtcgagggc gacctgagcg atctgcccga gcgcggcgtc
     1801 gcggcctcgc gccagttggc gaagcgccag atcacctggt tgcgcggcat gccgcagcgc
     1861 catcgcgtgg cctgcgatcg cgacgacgcc gccgccgcgg tcgtcgcgct ggccaccacg
     1921 ctcgcggccg aactgccgcg cggctgaaat gagatttgcc gtatggagtt gtgatggtgt
     1981 ggcggctttc ggccctttgc cgacgctcga ctttggtttg cctcggccgg cgcggcaaag
     2041 gcatgcccgg gcaggctgct ccgcttcact gcggcggtcg tcgctgcgcg ccgactgccc
     2101 tgcgctgctc ggtcccgtgg cccgtcgcca aactcacttc gctc
//
LOCUS       DATMJB010000544         2461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_258973, whole genome shotgun sequence.
ACCESSION   DATMJB010000544 DATMJB010000000
VERSION     DATMJB010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258973"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VNU48_11650"
     CDS             complement(<1..939)
                     /locus_tag="VNU48_11650"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWI11975.1"
                     /translation="MTVKSNTASEGWYAHSERTSQTDDQRIKDVMPLPPPEHLIRFFP
                     IAGTPVETLIGDTRQAIRRIMQRKDDRLLVIIGPCSIHDPAAAVEYARRLKQQREKYK
                     DTLEIVMRVYFEKPRTTVGWKGLINDPYLDESYRIDEGLRIARQLLLEINRNGVPAGS
                     EFLDVISPQYIGDLISWGAIGARTTESQVHRELASGLSAPIGFKNGTDGNIRIAIDAI
                     QAAMRPHHFLSVHKNGQVAIVETKGNKDCHVILRGGKTPNYDAAHVAAACHELEAARL
                     PCTLMVDCSHANSSKQHERQVDVARDIAGQIAGGSRH"
     gene            complement(1178..>2461)
                     /gene="tldD"
                     /locus_tag="VNU48_11655"
     CDS             complement(1178..>2461)
                     /gene="tldD"
                     /locus_tag="VNU48_11655"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HWI11976.1"
                     /translation="SGSFGIDQGVGVRAVAGEKTAFAYSDDISEAALLDAATTVRTIA
                     AAGQSRQVKVGTKQKVGGSRVLYAPMDPIATLDSTQKVALLEKVEKLARSKDPRIVQV
                     MAGLAGEYDVVMIARADGTLAADVRPLVRLSVTVIAEQTIAGEVRREVGSGGGGGRFG
                     FGYFHDGVIEDYVNDAVNAALTNLESRPAKAGEMSVVLGPGWPGVLLHEAIGHGLEGD
                     FNRKGSSAFSGKVGQRVAAKGVTVLDDGTIPDRRGSLNVDDEGNPSQRNVLIEDGILK
                     GYIQDSMNARLMGVALTGNGRRESYAAVPMPRMTNTYMLGGDKTKEEIVAGLKRGLYA
                     TNFGGGQVDITSGKFVFSASEAFWVENGRVQYPVKGATIIGNGPDALSRVSAIGNDMK
                     LDPGVGVCGKEGQSVPVGVGQPTLRIDRLTVGGTA"
BASE COUNT          341 a          853 c          814 g          453 t
ORIGIN      
        1 gtggcggctg ccgccggcaa tctgcccggc gatgtcgcgc gccacgtcca cctgccgctc
       61 gtgctgcttg ctgctgttgg catgcgagca gtcgaccatc agggtgcaag gcaggcgcgc
      121 ggcctccagc tcgtggcagg cggccgcgac atgcgccgcg tcgtagttcg gcgtcttgcc
      181 gccgcgcagg atcacgtggc agtccttgtt gcccttggtc tcgacgatcg cgacctggcc
      241 gttcttgtgc accgacagaa agtggtgcgg ccgcatcgcc gcctgaatgg cgtcgatcgc
      301 gatccggatg ttgccgtcgg tgccgttctt gaagccgatc ggcgccgaca gccccgaggc
      361 gagttcgcgg tgcacctggc tttcggtcgt gcgcgcgccg atcgcgcccc acgagatcag
      421 gtcgccgatg tactgcggcg agatcacgtc gaggaattcg ctgccggcgg gcacgccgtt
      481 gcggttgatc tccagcagca gctggcgcgc gatgcgcagg ccctcgtcga tgcggtagct
      541 ctcgtccagg tacgggtcgt tgatcaaccc cttccagccg accgtggtgc gcggtttctc
      601 gaaatacacg cgcatcacga tctcgagcgt gtccttgtat ttctcgcgct gctgcttcag
      661 cctgcgcgcg tactcgaccg ccgccgccgg atcgtggatc gagcacggcc caatgatcac
      721 gagcagccgg tcgtccttgc gctgcatgat gcggcggatc gcctggcgcg tgtcgccgat
      781 cagcgtctcg accggcgtgc cggcgatcgg gaagaagcgg atcaggtgct cgggcggcgg
      841 cagcggcatc acgtccttga tgcgctggtc gtcggtctgg ctggtgcgtt cgctgtgtgc
      901 ataccaaccc tcgctggcgg tgttcgattt gacggtcatg gcagttggct cctggtttcg
      961 tgatcggtgc gcgcaggcga aaaaaaaccg ccgggggtga gcccggcggt ttctggagat
     1021 tcgttgcgcg ctgtgtgctt atgcgctggc ctctccggcc gccgagcggt gcgagaacca
     1081 aaagtacgca aagaaatgcg ggcagcggga aagcatgtgc gcaaatgtag catgctcggc
     1141 gagcgcccca gcagacggtg cgggtcgagg gggcgctcta ggccgtgccg ccgacggtga
     1201 gccggtcgat gcgcagcgtc ggctgaccga cgcccaccgg cacgctctgc ccttccttgc
     1261 cgcacacgcc cacgcccggg tcgagcttca tgtcgttgcc gatggcgctg accctgctca
     1321 gcgcgtcggg cccgttaccg atgatggtcg cgcccttcac cgggtactgg acccgcccat
     1381 tctcgaccca gaacgcctcg ctcgccgaga acacgaactt gccgctggtg atgtcgacct
     1441 ggccaccgcc gaagttggtc gcgtacaggc cgcgcttcag gccggcgacg atctcttcct
     1501 tggtcttgtc gccgcccagc atgtaggtgt tggtcatgcg cggcatcggc accgcggcgt
     1561 agctctcgcg ccggccgttg ccggtgagtg ccacgcccat caggcgggca ttcatcgagt
     1621 cctggatgta gcccttcagg atgccgtcct cgatcagcac attgcgctgg ctcgggttgc
     1681 cttcgtcgtc gacgttcagc gagccgcgac gatcgggaat ggtgccgtcg tcgagcaccg
     1741 tcacgccctt cgcggcgaca cgctggccga ccttgccgct gaaggcgctc gatcccttgc
     1801 ggttgaagtc gccctccagg ccgtggccga tcgcctcgtg cagtagcacg ccgggccagc
     1861 ccggcccgag cacgacgctc atctcgccgg ccttcgccgg gcgcgattcg aggttggtca
     1921 gcgcggcatt cacagcgtcg ttgacgtagt cttcgatcac accatcatga aaatagccga
     1981 acccgaagcg cccgccaccg ccgcccgaac cgacctcgcg gcgcacctcg ccggcgatcg
     2041 tctgctcggc gatcacggtc acgctcagcc gcaccagcgg ccgcacatcg gccgccaagg
     2101 tgccgtcggc gcgcgcgatc atcacgacgt cgtactcgcc ggccaggccc gccatcacct
     2161 gcacgatgcg cgggtccttg ctgcgcgcga gcttctcgac cttctcgagc agcgcgacct
     2221 tctgggtgct gtcgagcgtg gcgatcggat ccatcggcgc gtacagcacg cggctgccgc
     2281 cgaccttctg cttcgtgccg accttgacct ggcggctctg gcccgcggcc gcgatcgtgc
     2341 gcacggtggt cgccgcgtcg agcagcgccg cttcggagat gtcgtccgag tacgcgaatg
     2401 cggtcttctc gcccgccacc gcgcgcacgc ccacaccctg gtcgatgccg aagctgccgc
     2461 t
//
LOCUS       DATMJB010000545         3274 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_4956, whole genome shotgun sequence.
ACCESSION   DATMJB010000545 DATMJB010000000
VERSION     DATMJB010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4956"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="VNU48_11660"
     CDS             <1..1094
                     /locus_tag="VNU48_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HWI11977.1"
                     /translation="RAGFEAVRTLTAPAVAKGRMTELQQAALLNLITPTGEAADLQGC
                     DLIIEAVFEQRALKAAVTREAEAMLARDGVFASNTSTLPITGLAQASARPEKFIGLHF
                     FSPVHKMKLVEIIRGAATSDETVARAFDVVLALGKLPIVVNDSRGFFTSRVFGSFVME
                     GAAMLAEGIPAPLIEHAALAAGMPVGPLAVLDETSPALSVHVMDQTRADLAAEGRTHQ
                     AGRGEALVERMVRELKRPGRAGGGGFYDYPPGEPKRLWPQLRTLFEKTGEPLPAIDEL
                     KQRLLYRQAIETARCLHEGVLTSSADANIGSIFGIGFPAWTGGAAQFVESEGRARFVA
                     NADALAAKFGAGFSLSAELRERLEAAPRA"
     gene            complement(1152..1451)
                     /locus_tag="VNU48_11665"
     CDS             complement(1152..1451)
                     /locus_tag="VNU48_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWI11978.1"
                     /translation="MKSLVVLLVAAFAAPAFAQADFAQKKNCMACHAIDKKVLGPAYK
                     DVAAKYADQKDAVDKLAAKVVKGGTGVWGNIPMPANPQVTEAEAKQLVTWILTLK"
     gene            1567..1974
                     /locus_tag="VNU48_11670"
     CDS             1567..1974
                     /locus_tag="VNU48_11670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04438 family Trp-rich protein"
                     /protein_id="HWI11979.1"
                     /translation="MFFVVLGCVLLGLKYFEFGPVGAWPWWLVLAPFGLAMLWWWYAD
                     VSGWTKRREMDRMEERKATRRRTAMERLGLKYSSANKADRRAQKFLEARQREIDKIEG
                     KRAEKRKRDRDSILGSRFDSEAAASQPAEPKKP"
     gene            complement(1985..>3274)
                     /locus_tag="VNU48_11675"
     CDS             complement(1985..>3274)
                     /locus_tag="VNU48_11675"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HWI11980.1"
                     /translation="MGMLRANVPAIYVYGGTILPGRYKGQDLNIVSVFEAVGQFSAGN
                     MSEEDFCQIERRAIPGTGSCGGMYTANTMSSAFEALGMSLPFSSTMANPHDEIVAHTS
                     EAARVLVEAVKKDLKPRDIVTRKSIENAVAVIMATGGSTNAVLHFLAIAHAAQVEWTI
                     DDFERVRQKVPVLCDLKPSGKYLAVDLHRAGGIPQVMKVLLKAGLLHGDCITITGKTV
                     AENLAAIPDLPRADQDVIRAIDQPMYAHGHLAILKGNLSPEGCVAKITGLKSPVITGP
                     ARVFEDEQSALAAIMAKRIVAGDVMVLRYLGPKGAPGMPEMLAPTGALVGQGLGESVG
                     LITDGRFSGGTWGMVVGHVAPEAYAGGNIALIREGDSITIDAHTLTLQLNVDAAELAQ
                     RRAAWQAPAPRYTRGVLAKFARNASSASTGAVLDGDL"
BASE COUNT          477 a         1089 c         1171 g          537 t
ORIGIN      
        1 cccgcgccgg cttcgaggcc gtgcgcacgc tgaccgcgcc ggccgtcgcc aagggccgca
       61 tgaccgagct gcagcaggcc gcgttgctga acctgatcac gccgaccggt gaggctgccg
      121 atctgcaggg ttgcgacctg atcatcgagg cggtgttcga gcagcgcgcg ctgaaggccg
      181 cggtgacgcg cgaggccgag gccatgctgg cgcgcgacgg cgtgttcgca tcgaacacct
      241 cgaccttgcc gatcaccggg ctggcgcagg ccagcgcgcg gcccgagaag ttcatcggcc
      301 tgcacttctt ctcgccggtg cacaagatga agctggtcga gatcatccgc ggcgcggcga
      361 cctccgacga gaccgtggcg cgtgccttcg acgtcgtgct cgcgctgggc aagctgccga
      421 tcgtcgtcaa cgattcgcgc ggcttcttca ccagccgcgt gttcggcagc ttcgtgatgg
      481 agggcgcggc gatgctcgcc gagggcatcc cggcaccgct gatcgagcac gcggcgctgg
      541 ccgccggcat gccggtcggg ccgctcgcgg tgctcgacga gacctcgccc gcgctgtcgg
      601 tgcacgtgat ggaccagacg cgcgccgatc tcgcggccga gggacgaacc catcaggccg
      661 gccgcggcga ggcgctggtc gaacgcatgg tcagggagct gaagcgcccg ggccgcgccg
      721 gcggcggtgg tttctacgac tacccgccgg gcgagccgaa gcggctgtgg ccgcagctgc
      781 gcacgctgtt cgagaagacg ggcgagccgt tgccggcgat cgacgaactc aagcagcggc
      841 tgctgtaccg ccaggcgatc gagaccgcgc gctgcctgca cgaaggcgtg ctgacgagtt
      901 ctgccgacgc gaacatcggc tcgatcttcg gcatcggctt cccggcctgg accggcggcg
      961 cggcgcagtt cgtcgagtcg gagggaaggg cgcgtttcgt tgcgaacgcg gatgcgctgg
     1021 cagcgaagtt cggtgccggc ttcagcttgt cggcggaatt gcgcgaacgg ctggaagcgg
     1081 cgccgcgcgc gtagcgcgca acgaaaaagg ccccgcgtgc ggggccttcg tttcgtgcgc
     1141 gatcgcgacg tttacttcag cgtcaggatc caggtgacca gctgcttggc ctcggcctcg
     1201 gtgacctggg ggttggccgg catcgggatg ttgccccaga cgccggtgcc gcccttgacg
     1261 accttggcgg cgagcttgtc gaccgcgtcc ttctggtcgg cgtacttggc ggccacgtcc
     1321 ttgtaggccg ggcccagcac cttcttgtcg atcgcgtggc aggccatgca gttcttcttc
     1381 tgggcgaagt cggcctgcgc aaaggcgggt gccgcgaacg cggcgaccag caaaacaacc
     1441 agtgatttca tgtcttactt caccccgcag ggcgccctgt ttgaactaca ttgcgttcaa
     1501 cgcattgtag cgttccgggg ttgacgcccg tacacgggcg gactcacgaa cggttggagg
     1561 gtcgagatgt tttttgtcgt gctgggctgt gtgctgctgg ggctgaagta cttcgagttc
     1621 gggccggtcg gggcatggcc ctggtggctg gtgctcgcgc cgttcgggct ggcgatgctg
     1681 tggtggtggt acgccgacgt atcgggctgg accaagcgcc gcgagatgga ccgcatggaa
     1741 gagcgcaagg ccacccgccg ccgcacggcg atggagcggc tcggcctgaa gtacagctcg
     1801 gccaacaagg ccgatcgccg ggcccagaag ttcctcgagg cgcgccagcg cgagatcgac
     1861 aagatcgaag gcaagcgtgc cgagaagcgc aagagggacc gcgactcgat cctgggcagc
     1921 cgcttcgaca gcgaggccgc cgccagccag cccgccgagc cgaagaagcc ctgaaccgcc
     1981 ggcgtcagag gtcgccgtcg agcaccgcgc cggtgctggc gctcgacgcg ttcctcgcga
     2041 acttggccag cacgccacgc gtgtagcgcg gcgccggtgc ctgccacgcg gcgcggcgct
     2101 gggccagctc ggcggcgtcg acgttgagtt gcagcgtcag cgtgtgggcg tcgatcgtga
     2161 tcgagtcgcc ctcgcggatc agcgcgatgt tgccgccggc ataggcctcg ggcgcgacat
     2221 gcccgaccac catgccccag gtgccgccgg aaaagcgccc gtcggtgatc aggccgaccg
     2281 attcacccag cccctggccg accagcgcgc cggtgggcgc gagcatctcg ggcatgccgg
     2341 gggcgccctt cgggccgagg tagcgcagca ccatcacgtc gccggccacg atgcgcttgg
     2401 ccatgatcgc ggccagcgcc gactgttcgt cctcgaacac gcgcgccggg ccggtgatca
     2461 ccgggctctt cagcccggtg atcttggcga cgcagccctc gggcgagagg ttgcccttca
     2521 ggatcgcgag gtggccgtgc gcgtacatcg gctggtcgat agcgcggatc acgtcctgat
     2581 cggcgcgcgg cagatcgggg atcgccgcga ggttctcggc caccgtcttg ccggtgatcg
     2641 tgatgcagtc gccgtgcagc aggccggcct tcaacagcac cttcatcacc tgcgggatgc
     2701 cgccggcgcg gtgcagatcg accgcgaggt acttgccgga gggcttcagg tcgcacagca
     2761 ccggcacctt ctggcgcacc cgctcgaagt cgtcgatggt ccattcgacc tgcgccgcgt
     2821 gcgcgatcgc gaggaagtgc agcaccgcgt tggtcgagcc gccggtggcc atgatcaccg
     2881 cgacggcgtt ctcgatgctc ttgcgcgtga cgatgtcgcg cggcttcaga tccttcttca
     2941 ccgcctcgac gagcacgcgc gcggcctcgc tggtgtgcgc gacgatctcg tcgtgcgggt
     3001 tggccatcgt gctcgagaac ggcaggctca tgcccagcgc ctcgaaggcc gagctcatcg
     3061 tgttggcggt gtacatgccg ccgcacgatc cggtgccggg gatcgcgcgg cgctcgatct
     3121 ggcagaagtc ttcctcgctc atgttgccgg cgctgaactg gccgacggcc tcgaacacgc
     3181 tgacgatgtt caggtcctgc cccttgtagc ggcccggcag gatggtgccg ccgtagacgt
     3241 agatggccgg cacgttggcg cgcagcatgc ccat
//
LOCUS       DATMJB010000546         2379 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_141424, whole genome shotgun sequence.
ACCESSION   DATMJB010000546 DATMJB010000000
VERSION     DATMJB010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141424"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            273..536
                     /locus_tag="VNU48_11680"
     CDS             273..536
                     /locus_tag="VNU48_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11981.1"
                     /translation="MNPTQMPDRRGAVRGDARRPGAPIAREGTARGKADWVARCARRL
                     RVLNPACDVANAHALALDLWAEVGYFDPEMAAELELESSATDD"
     gene            529..1419
                     /locus_tag="VNU48_11685"
     CDS             529..1419
                     /locus_tag="VNU48_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HWI11982.1"
                     /translation="MTEPGGTETRPPRFDYGESFSRNLGWVTEAEQARLRGARVCIAG
                     LGGVGGVYLLTLARLGVGAFSVADFDHYALANFNRQIGATVSSLGRSKLEVMVAMARD
                     INPELDIRRFDQGVDATNVDAFLADGDVYLDGLDFFALDIRRRVFAACAAKAIPATTV
                     APLGMGAALLNFVPGGMSFEQYFRLEGRTPHEQALRFLVGLSPAMLQMSYLVDPSRVD
                     LAQRRGPSTVIAVQLCSGIAAAQVLKLILKRGDVIAAPAGLHFDAFRNKLKSTWRPGG
                     NAHPAQRLLLALAARRLRRR"
     gene            1423..>2379
                     /locus_tag="VNU48_11690"
     CDS             1423..>2379
                     /locus_tag="VNU48_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11983.1"
                     /translation="MRPSADDLRQILAAGVQAPSAENRHAIVFLMREQDVELTATDSA
                     SWAALPHRAMLALLACGAVLENMALRSAQMGYAMQADILPEPSQPTLIARLDWQRAGA
                     RDALSDQIEARHTNRRLFRSTRAAPEILDRLLQACRAVAGADLLWLDARETRSLALHA
                     IRIAETERFKRARLHEEIFSAVRFEAGWSGPTAEGLAPATLEVEAPMRPLFAALRHWP
                     TMRRATWFGVPLALGFRAGYLPCASAPHLGLIVGRDGARGNLDAGRAMQRIWLAATAE
                     GLSLQPMAAATALARQVPGDGWVAPATRQLPAGALERLTGASR"
BASE COUNT          341 a          824 c          843 g          371 t
ORIGIN      
        1 cgacggccac gtgaggggtg tttgaacagg catgacggac tccttgggtg gggcggaaca
       61 gatacacatt gcccgcgcgg cggacgattg aatttggcga cgcccgggcg cgccgctgtg
      121 taggagaacg ccttgtccac aggctacccc ccttgaacgc gggggtcgcg tcgcaggcac
      181 tcggctgacg cctgggggtg gacgcgtcct acgtgcatct cttgtcccga agtggagact
      241 tttgaacccg aatgcctccg tgggagagcc tcatgaaccc gacgcaaatg ccggatcgcc
      301 gcggcgctgt tcgcggcgat gcgcgtcgcc ccggtgcgcc gatcgccaga gaaggcaccg
      361 cacgcggcaa ggccgactgg gtcgcccgct gcgccagacg cctgcgggtg ctgaatccgg
      421 cctgcgatgt cgcgaacgcc catgcactgg cgctggacct gtgggccgag gtgggctact
      481 tcgatcccga gatggccgcc gaactggaac tcgaatcttc ggcaaccgat gactgagccg
      541 ggcggcactg aaacgcggcc gccaagattc gactacggcg aatcgttctc gcgcaatctt
      601 ggctgggtca cggaggccga gcaggcacga ctgcgcggcg cacgcgtgtg catcgcgggg
      661 ttgggcgggg tcggtggcgt gtacctgctg acgctggcgc gcctcggtgt cggcgcgttc
      721 tcggtcgccg atttcgacca ctatgccctc gcgaatttca atcggcagat tggggccacc
      781 gtctcgtcgc tggggcgatc gaagctggag gtgatggtcg ccatggcgcg cgacatcaac
      841 cccgagctcg acatccgtcg cttcgaccag ggcgtcgacg ccaccaacgt cgacgccttc
      901 ctcgccgacg gcgacgtcta cctcgacggc ctggactttt tcgcactcga catccgccgc
      961 cgcgtcttcg cggcctgcgc cgccaaggcg atccccgcga cgaccgtggc gccgttgggc
     1021 atgggtgccg cgctgctcaa cttcgtgccc ggcggcatga gcttcgagca gtatttccgg
     1081 ctcgaagggc gcacgccgca cgagcaggcg ttgcggtttc tcgttggcct gtcgccggcg
     1141 atgctgcaga tgtcctacct ggtcgacccg tcacgcgtcg atctggccca gcgccgcggc
     1201 ccatcgaccg tgatcgcggt ccagctgtgc agcggcatcg cggccgcgca ggtcctgaag
     1261 ctgatcctga aacgcggcga cgtgatcgcc gcgccggccg gcctgcattt cgacgcgttc
     1321 cgaaacaagc tgaaaagcac atggcgcccg ggcggcaacg cgcacccggc gcagcgcctg
     1381 ctgctcgcgt tggcggcgcg gcgcctgcgg cgtcgctgac cgatgcgacc gtcggccgac
     1441 gatctgcgcc agatactcgc cgctggcgtg caggcgccct cggccgagaa tcgccatgcc
     1501 atcgtgttcc tgatgcgcga gcaggacgtg gaattgacgg ccaccgacag cgcctcgtgg
     1561 gccgcactgc cgcatcgggc gatgctcgcg ttgctggcct gcggcgcggt gctcgagaac
     1621 atggcgctgc gttcggcgca gatgggctac gcgatgcagg cagacatcct gcccgaaccg
     1681 tcacagccga cactgatcgc gcgcctcgac tggcagcgcg ccggcgcgag ggacgcactt
     1741 tcggaccaga tcgaggcgcg ccataccaac cgccgcctgt tcaggtcgac gcgggccgcg
     1801 cccgagatcc tcgaccggct gctacaggcg tgccgcgccg tggcgggcgc cgacctgctc
     1861 tggctcgacg cgcgcgagac ccggtcgctc gcgctgcacg ccatccggat cgccgaaacc
     1921 gagcgcttca agcgagccag actgcacgag gagattttca gtgcggtgcg gttcgaggcc
     1981 ggctggagcg ggccgaccgc ggaagggctc gcgccggcca cgctcgaagt cgaagcgccg
     2041 atgcggccgc tgttcgcggc gctgcgccac tggccgacga tgcggcgcgc cacgtggttc
     2101 ggtgtgccgc tcgcgctggg gtttcgcgcc ggctacctgc cgtgtgcgag tgcgccgcac
     2161 ctcgggctga tcgtcggccg cgacggtgcg cgcggcaacc tcgacgccgg tcgggcgatg
     2221 cagcgcatct ggctcgctgc gaccgccgag ggcctgagcc tgcaaccgat ggccgccgcg
     2281 accgcgctgg cgcggcaggt gcccggcgac ggatgggtgg cgccggcgac ccgccagctt
     2341 cctgcgggag cgctcgaacg cctgacgggc gcctcgcgg
//
LOCUS       DATMJB010000547         2094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_259536, whole genome shotgun sequence.
ACCESSION   DATMJB010000547 DATMJB010000000
VERSION     DATMJB010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259536"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VNU48_11695"
     CDS             complement(<1..461)
                     /locus_tag="VNU48_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HWI11984.1"
                     /translation="MQAILAVTLPFFALVLLGYLAAQREVLPEDAIPGLNVYVLFFAL
                     PCMLFRFGSSQPFARLIDVRLIAIYAVCAVAMVAATIALTWQRGARPGVDMKNAAFGA
                     LVAAFPNAGFMGVPLLVALLGDAAAGPVIGAILVDLILTSTLCLALAQAST"
     gene            523..>2094
                     /locus_tag="VNU48_11700"
     CDS             523..>2094
                     /locus_tag="VNU48_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWI11985.1"
                     /translation="MSLVPDPLNPAQLEAVHHLGGPCLVLAGAGSGKTRVITHKIARL
                     LQAGYEPGQIAAITFTNKAAQEMRERAKALVGPRAAKHLVVSTFHSLGVRILRSEFER
                     VGLKQQFSILDSDDVLGILKEAGASTDNALARRWQWAISGWKNQGLSSASVAPANDDE
                     RVAQRVMQRYEERLAAYQAVDFDDLIGLPHRLLLRDAEVRAKWQDTLRYLLVDEYQDT
                     NAIQYEMLKSLAGHPGDKDSALFTAVGDDDQSIYGWRGATIDNLKRLPQDYPALKVIA
                     LEQNYRSTGHILRAAKKVIAHNPKLFEKKLWSAFGDGEPVRVIECDNEEHEAERVVTR
                     FQALQAGTVASASAASPTGASAVEFKDFAVLYRANHQARVFEKALRKAGIPYKVSGGQ
                     SFFDRAEIKDLCAWLRLIVNNDDDPAFLRAVSTPKRGIGHQTLASLGEFSGRWKTSLY
                     EALFAESLGSVLSGRALGSLHEFGRYVNDLEYRARHTVGAEDAKGLLLGWLKDIAYEK
                     HLYDGEESEKLAAARW"
BASE COUNT          371 a          730 c          722 g          271 t
ORIGIN      
        1 tgcgtgctcg cctgcgccag cgccaggcac agcgtgctgg tcaggatcag gtcgaccagg
       61 atcgcgccga tcaccgggcc ggccgcggcg tcgccgagca gcgcgaccag cagcggcaca
      121 cccatgaagc cggcgttcgg gaacgccgcc accaacgcgc cgaaggcggc gttcttcatg
      181 tccacgcccg gccgcgcgcc gcgctgccag gtcagcgcga tcgtcgccgc gaccatcgcc
      241 actgcgcaga cggcgtagat cgcgatcagc cgcacgtcga tcaggcgtgc gaacggctgg
      301 ctcgagccga agcggaacag catgcagggc agcgcgaaga acagcacgta gacgttcagg
      361 ccggggatcg cgtcctcggg cagcacctcg cgctgcgcgg cgaggtagcc gagcagcacc
      421 agcgcgaaga acggcagcgt gacggcgagg atcgcttgca tcgtgcgccg agtatcgcaa
      481 ggcggcgtcg ctatcatggc gccaaccccg cctcccgctc tcgtgtccct cgttcccgat
      541 cccctcaacc ccgcgcagct cgaagcggtc catcacctcg gcggcccgtg cctggtgctc
      601 gccggcgccg gctccggcaa gacgcgcgtg atcacgcaca agatcgcgcg gctgctgcag
      661 gccggctacg aacccggcca gatcgcggcg atcacattca ccaacaaggc cgcccaggag
      721 atgcgcgagc gcgccaaggc gctggtcggc ccgcgcgccg cgaagcatct cgtcgtcagc
      781 accttccatt cgctgggcgt gcgcatcctg cgcagcgagt tcgagcgcgt cgggctgaag
      841 cagcagttca gcatcctcga cagcgacgac gtgctcggta tcctgaagga ggccggcgcg
      901 agcaccgaca acgcgctggc gcggcgttgg cagtgggcga tcagcggctg gaagaaccag
      961 ggcctgagca gcgcgagcgt cgcgccggcg aacgacgacg agcgcgtcgc gcagcgcgtg
     1021 atgcagcgct acgaggaacg tctcgcggcc taccaggcgg tcgacttcga cgacctgatc
     1081 gggctgccgc accggctgct gctgcgcgac gccgaggtcc gcgcgaagtg gcaggacacc
     1141 ctgcgctacc tgctcgtcga tgagtaccag gacaccaacg cgatccagta cgagatgctg
     1201 aagtcgctcg ccggccatcc cggtgacaaa gacagtgcgc tgttcaccgc ggtcggcgac
     1261 gacgaccaga gcatctacgg ctggcgcggc gcgaccatcg acaacctgaa gcgcctgccg
     1321 caggactacc cggcgctgaa ggtcatcgcg ctcgaacaga actaccgctc gaccggccac
     1381 atcctgcgcg ccgcgaaaaa ggtgatcgcg cacaacccga agctgttcga gaagaagctc
     1441 tggagcgcgt tcggcgacgg cgagccggtg cgcgtgatcg agtgcgacaa cgaggagcac
     1501 gaggccgagc gggtcgtcac gcggttccag gcgttgcagg ccggcacggt cgcgagcgcc
     1561 tcggccgcgt cgccgaccgg tgcgagcgcg gtcgagttca aggacttcgc ggtgctgtac
     1621 cgcgcgaacc accaggcgcg cgtgttcgag aaggcactgc gcaaggccgg cattccgtac
     1681 aaggtctcgg gcgggcagag cttcttcgac cgcgccgaga tcaaggacct gtgcgcgtgg
     1741 ctgcgcctga tcgtcaacaa cgacgacgac cccgcgttcc tgcgtgcggt gtcgacgccg
     1801 aagcgcggca tcggccacca gacgctcgcc agcctcggcg agttttccgg ccgctggaag
     1861 acgagcctgt acgaagcgct gttcgccgaa agcctcggct cggtgctgag cggccgcgcg
     1921 ctcggctcgc tgcacgagtt cggccgctac gtgaacgacc tcgagtaccg cgcgcgccac
     1981 accgtcggcg ccgaagacgc gaaggggctg ctgctcggct ggctgaagga catcgcctac
     2041 gagaagcacc tgtacgacgg cgaggagagc gagaagctcg ccgccgcgcg ctgg
//
LOCUS       DATMJB010000548         3626 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_795300, whole genome shotgun sequence.
ACCESSION   DATMJB010000548 DATMJB010000000
VERSION     DATMJB010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795300"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VNU48_11705"
     CDS             complement(<1..383)
                     /locus_tag="VNU48_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI11986.1"
                     /translation="MKKMKLVMVGNGMAGVRTLEELLKIAPDLYDITVFGAEPHPNYN
                     RILLSPVLAGEQTVDEIVLNSWEWYAENNITLHAGKKVVEVDRVKRIVRAEDGTEAEY
                     DRLLMCTGSNPFMLPVPGKDLKGVIA"
     gene            458..2218
                     /locus_tag="VNU48_11710"
     CDS             458..2218
                     /locus_tag="VNU48_11710"
                     /EC_number="2.7.11.-"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-serine/threonine
                     kinase/phosphatase"
                     /protein_id="HWI11987.1"
                     /translation="MSFDVDIGHFSLQGPRERNEDFAAAARPAAGDATRGVIAAIADG
                     VSGGGAGREAAQTSVMSVVGDYHSAPDTWETSVVLDRLIGAQNAWLADHNRRRQGARD
                     GADAPLAMSTLTVLVLRGQAWMLAHVGDTRAWLLRDAQGATDCVQLTQDHAFEEAFQR
                     NRLTRAMGLDDRVRVDFEQGELRIGDTFVLTSDGVHGVLDRTRLQALAGSHPGAQAAS
                     EAIVHAAIAAGTRDNASALVLRVNGLDASRFEDALLRGRGLPALPRRKVGDTVDGYVV
                     TAPLADTGVHRLYQARATASGALVVLKTLHESRAHDAQERAMLLHEAWLASRVAERTP
                     PRGEAGFVCTHEPDRPSAFYTVFDWHAGVTLEAMLEQGRRFEVGDIVEGAIAVGRALA
                     RLHRHGVIHRDIKPGNLYHGDDGLWRVLDLGAALSGSDPGALRALHAGTPSYMNPEQW
                     GLADTAPRPADAGSDLYALGVTLYRWLTGKLPYGEIEPYQGGRFRRDPKPPSRLRPDV
                     PIWLDHVVLKAVALDPRQRFETAEELVLALERGASRPLAALAGTPLLARHPTALWQIA
                     LGASLLFNALLIYWLLFLPR"
     gene            complement(2245..3519)
                     /locus_tag="VNU48_11715"
     CDS             complement(2245..3519)
                     /locus_tag="VNU48_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI11988.1"
                     /translation="MSSIKTFARSGHWPTLVASFLYFDFTFAIWVLNGAMAPFISETF
                     ALDAAQKGFMVSVPIIAGALMRFPLGVLSQYIGRKNAAMVEMVLIIVALIYGFVFVDS
                     YDGVLAMGVLLGIAGGSFGVALSLGSGWFPPKYKGLAMGIAGAGNSGTVLAVLFAPPL
                     AMKYGWTTVYGLAAATMVLPMIVMWFAAKEPPDREHQTLREHVACLFEKDGCAFSLIY
                     IITFGGFVGLASFLPSYFHDQFKVTKVEAGQLTMLATLMGSAVRVVGGYVSDRIGGIA
                     TLSGVLILVIVTLVLCGMAGQSVALTTLLFMVCFAALGAGNGALFQLVPLRWPLTTAV
                     AGSMIGEIGALGGGFIPNAMGQSKQLTGSYFWGFVAFAVLAALMLVMLRVVQIRWTRT
                     WAEKGGRARPPVAHADMPDYGASGLRSGKVVL"
BASE COUNT          572 a         1305 c         1263 g          486 t
ORIGIN      
        1 gcgatcacgc ccttcaggtc cttgccgggc accggcagca tgaaggggtt ggagccggtg
       61 cacatcagca gccggtcgta ctcggcctcg gtgccgtcct cggcgcgcac gatgcgcttg
      121 acgcggtcga cctccaccac cttcttgccg gcgtgcaggg tgatgttgtt ctcggcatac
      181 cactcccagc tgttgagcac gatctcgtcg accgtctgct cgcctgcgag caccggcgac
      241 agcaggatgc ggttgtagtt ggggtgcggc tcggcgccga acacggtgat gtcgtagaga
      301 tcgggcgcga tcttcaacag ctcctcgagg gtgcgcacgc cggccatgcc gttgcccacc
      361 atcaccagct tcatcttctt catcgcggac tccgaaccgg ggttcttgaa aaattctggc
      421 cgcacgcgcc gcgccgcgtc gtcgatactg cgcggccatg agcttcgacg tcgacatcgg
      481 ccacttcagc ctgcaggggc cgcgcgagcg caacgaggat ttcgccgcgg ccgcccggcc
      541 cgcggcgggc gatgcgacgc gcggcgtgat cgccgcgatc gccgacggcg tcagcggcgg
      601 cggcgccggc cgcgaggcgg cgcagaccag cgtgatgagc gtcgtgggcg actatcacag
      661 cgcccccgac acctgggaga cctcggtcgt gctcgaccgc ctgatcggcg cgcagaacgc
      721 ctggctggcc gatcacaacc ggcgccgcca gggcgcgcgc gacggcgccg acgcgccgct
      781 cgcgatgagc acgctgacgg tgctggtgct gcgcggccag gcctggatgc tggcgcacgt
      841 cggcgatacg cgcgcctggc tgctgcgcga cgcgcagggc gcgaccgact gcgtccagct
      901 gacccaggac cacgcgttcg aagaggcctt ccagcgcaac cgcctgaccc gcgcgatggg
      961 cctggacgac cgcgtgcgcg tcgacttcga gcagggcgag ctgcgcatcg gcgacacctt
     1021 cgtgctgacc agcgacggcg tgcacggcgt gctcgaccgc acccgcctgc aggcgctggc
     1081 cggcagccac cccggcgcgc aggcggccag cgaggcgatc gtgcacgcgg cgatcgcggc
     1141 cggcacgcgc gacaacgcga gcgcgctggt gctgcgcgtc aacggcctcg acgcgagccg
     1201 cttcgaggac gcgctgctgc gcggccgcgg cctgccggcg ctgccgcgcc gcaaggtcgg
     1261 cgacacggtc gacggctacg tcgtcaccgc gccgctcgcc gacaccggcg tgcaccgcct
     1321 ctaccaggcg cgtgccaccg ccagcggtgc attggtcgtg ctcaagacgc tgcacgaatc
     1381 gcgcgcccac gacgcccagg agcgcgcgat gctgttgcac gaagcctggc tcgccagccg
     1441 ggtggccgag cgcacgccgc cgcgcggcga ggccggcttc gtctgcaccc acgaacccga
     1501 tcggccgagc gcgttctaca ccgtgttcga ctggcacgcc ggcgtcacgc tcgaggcgat
     1561 gctcgaacag ggtcggcgct tcgaggtcgg cgacatcgtc gagggcgcga tcgccgtggg
     1621 ccgggcgctg gcgcggctgc accgccatgg cgtgatccac cgcgacatca agccgggcaa
     1681 cctctaccac ggcgacgacg gcctgtggcg cgtgctcgac ctcggcgccg ccctgtcggg
     1741 cagcgacccc ggcgcgctgc gtgcgctgca cgccggcacg ccgagctaca tgaaccccga
     1801 gcagtggggc ctggccgaca ccgcgccgcg gcccgccgat gcgggcagcg acctctatgc
     1861 gctgggggtc acgctgtacc gctggctgac cggcaagctg ccgtacggcg agatcgagcc
     1921 ctaccagggc gggcgcttcc ggcgcgaccc gaagccgccg tcgcggctgc gccccgacgt
     1981 gccgatctgg ctcgatcatg tcgtgctcaa ggccgtcgcg ctcgaccccc ggcagcgctt
     2041 cgagaccgcc gaggaactgg tgcttgcact cgagcgcggc gcgtcgcgcc cgcttgcggc
     2101 gctcgccggc acgccgctgc tggcgcgcca cccgaccgcg ctgtggcaga tcgcgctcgg
     2161 cgcgtcgctg ctgttcaacg cgctgctcat ctactggctg ctgttcctgc cgcgctgaac
     2221 gcgcagcgcg atcggcgcgc ttcatcacag caccaccttg cccgagcgca ggccgctggc
     2281 gccgtagtcg ggcatgtcgg catgcgcgac cggcgggcgc gcgcgaccgc ccttctcggc
     2341 ccaggtgcgg gtccagcgga tctgcaccac gcgcagcatc accagcatca gcgccgcgag
     2401 caccgcgaac gcgacgaagc cccagaagta gctgccggtg agctgcttgg actgccccat
     2461 cgcgttcggg atgaagccgc cgccgagcgc accgatctcg ccgatcatcg aaccggcgac
     2521 cgccgtggtc agcggccagc gcagcggcac gagctggaac agcgcgccgt tgccggcacc
     2581 gagcgctgcg aagcagacca tgaacagcag ggtcgtgagc gccaccgact ggccggccat
     2641 cccgcacagc acgagcgtga cgatgaccag gatcagcacg ccgctgagcg tggcgatgcc
     2701 gccgatgcgg tcggagacgt agccgccgac gacgcgcacc gccgagccca tcagcgtggc
     2761 cagcatcgtg agctgcccgg cctcgacctt ggtgaccttg aactgatcgt ggaagtagct
     2821 cggcaggaag ctcgccagcc cgacgaagcc gccgaaggtg atgatgtaga tcaggctgaa
     2881 ggcacagccg tccttctcga acaggcaggc gacgtgctca cgcagggtct ggtgctcgcg
     2941 atcgggcggc tccttcgcgg cgaaccacat cacgatcatc ggcagcacca tcgtcgcggc
     3001 ggccaggccg tagaccgtgg tccagccgta cttcatcgcc agcggcggcg cgaacagcac
     3061 ggccagcacc gtgccggagt tgccggcacc ggcgatgccc atggccaggc ccttgtactt
     3121 cggcgggaac cagcccgagc cgagcgacaa cgccacgccg aagctgccgc cggcgatgcc
     3181 gagcagcacg cccatcgcga gcacgccgtc gtagctgtcg acgaacacga agccgtagat
     3241 cagcgcgacg atgatcagga ccatctcgac catcgccgcg ttcttgcggc cgatgtattg
     3301 cgacagcacg ccgagcggga agcgcatcag cgcgccggcg atgatcggca ccgacaccat
     3361 gaagcccttc tgcgcggcgt cgagtgcgaa ggtctcgctg atgaacggcg ccatcgcacc
     3421 gttgagcacc cagatcgcga aggtgaagtc gaagtagagg aaggacgcga ccagcgtcgg
     3481 ccagtggccg gagcgcgcga aggttttgat ggaggaaatg gatgacgaca cagcgggctc
     3541 cgggcagtgg ccgccggcag ggccggcgga agaggaggca catcagaccc acatcggtgt
     3601 ggacttgacc gcagtgcgtg agatcg
//
LOCUS       DATMJB010000549         7365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_502525, whole genome shotgun sequence.
ACCESSION   DATMJB010000549 DATMJB010000000
VERSION     DATMJB010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7365
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502525"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VNU48_11720"
     CDS             <1..301
                     /locus_tag="VNU48_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI11989.1"
                     /translation="YIVLRALGGREGIDLAKRFRPDLIALDLEMPEVNGFDVVEALKG
                     DAATARIPIVVVTAKDLTPADRSHLNGHILEIVGKAEFNHGRFIGEVQRALAKPA"
     gene            complement(665..752)
                     /locus_tag="VNU48_11725"
     tRNA            complement(665..752)
                     /locus_tag="VNU48_11725"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(716..718),aa:Ser,seq:cga)
     gene            complement(824..2137)
                     /gene="serS"
                     /locus_tag="VNU48_11730"
     CDS             complement(824..2137)
                     /gene="serS"
                     /locus_tag="VNU48_11730"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWI11990.1"
                     /translation="MLDITLLRKDLASVVARLDKRQSPQPFLDVDRFTALEAERRTIQ
                     VRTEELQARRNSLSKQIGQIKAQGGDSAATMAEVGGIGDTLKASAQRLDSIQTELSAL
                     LMALPNLPQDDVPVGPDETANVEVRRWGTPRVFDFPTRDHVDLGAPLGLDFDLGAKLS
                     GSRFSFLRGPVARLHRALAQFMLDVQTQEHGYTECYTPYIVNREILEGTGQLPKFKED
                     MFWVSRGGDETQPEQYLISTSEISLTNSVRERVLAADELPIKLTAHSPCFRSEAGSAG
                     RDTRGMIRQHQFDKVEMVQITHPDESDAALEQMVGHAEAVLRKLGLPYRVMLLSSGDM
                     GFGAAKTYDLEVWVPAQATYREISSCSNCEAFQARRMQARFRNAQGKNELVHTLNGSG
                     LAVGRTLVAVLENGQNADGSINVPEVLSPYLGGLAVLTSTKAAGA"
     gene            complement(2236..2460)
                     /locus_tag="VNU48_11735"
     CDS             complement(2236..2460)
                     /locus_tag="VNU48_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWI11991.1"
                     /translation="MIVCVCRRVSDHQIRQAAADGAHSLECLQFDLGVATQCGRCADC
                     ASRVLCDARSTGTHQQHVITTARPVQAQMA"
     gene            complement(2496..2972)
                     /gene="bfr"
                     /locus_tag="VNU48_11740"
     CDS             complement(2496..2972)
                     /gene="bfr"
                     /locus_tag="VNU48_11740"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HWI11992.1"
                     /translation="MKSDPQVLVHLNAQLKNELTAVNQYFLHYRMLKHWGLDKLAKKE
                     YEESIGEMKHADRLMERIFMLDGLPNLQDLGKLLIGENVPELLSCDLQSETGAQATIK
                     DGIAHCESVRDYVSRDVLQGILDDTEEHIDFLETQIDLIGKVGLENYLQSQMGEAS"
     gene            3196..4383
                     /locus_tag="VNU48_11745"
     CDS             3196..4383
                     /locus_tag="VNU48_11745"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HWI11993.1"
                     /translation="MLVRWSFMLGMFSALLLGGCGGGGGSARDASVPAVAALSAQAAL
                     GEKIFADVSLSASGRQACASCHDPDNARAPANDLSVQLGGPLLDQQGRRQAPSIRYLS
                     FNTAFFFASDGTPTGGFFWDGRSPSLAEQAGEPFVNPFEMANASVDEVIDRLSHASYA
                     ADFKAVFGADIFDRPVDAMARVQLALAQYQKEDTEFRAFSSKYDEFLRGNVALSEQEL
                     RGLALFNNPTKGNCIGCHPSGKSADGAFPLFTDFSYDNLGVPRNPGIARNDDPGYHDL
                     GLCERSDLAGRADLCGAFKVPSLRNVAQRHALFHNGRFATLKDAITFYVQRDTNPEKW
                     YPRNPDGSVNKFDDLPAQYRVNVNTAEVPYNRRPGDAPALSDAEVDDVIAFLATLNDG
                     YRR"
     gene            4528..4782
                     /locus_tag="VNU48_11750"
     CDS             4528..4782
                     /locus_tag="VNU48_11750"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HWI11994.1"
                     /translation="MLLNELPNGAFATVTRVVSTSSDVDAVALRRLAEIGFIPGEPVQ
                     LLRRGPGGREPLAVLIGDTMFGLRLLEAQCVEVRLEVRAA"
     gene            4790..6688
                     /locus_tag="VNU48_11755"
     CDS             4790..6688
                     /locus_tag="VNU48_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HWI11995.1"
                     /translation="MSQALNPAHAHAPWSVALVGNPNCGKTALFNLLTGARQKVANYA
                     GVTVERKVGTTRLPGGRSVSVIDLPGAYSLTPATPDELVTLEVIEGRRRGEDAPDAIV
                     AVVDATNLRMNLRLVLELKRLHRPMMVALNMADMARAQGLNIDVAKLGAELGCPVVET
                     IAVRHDGHAQLLALMEREFAAAPPEAVAGSVPFTPTSPAELQHEVRRILAIVEPGITT
                     RAANAARFHHRLDAVVMHPVWGLAILAVVLFLIFQAVFSWANVPMDAIKAAMAWLGAQ
                     TTTHMAEGPLRSLLVDGVIAGVGSVIVFLPQILILFFFILILEDSGYLPRAAFLLDNL
                     MGKVGLSGRAFIPLLSSFACAIPGIMATRTIANWKDRLATIMIAPLMTCSARLPVYAL
                     LIGAFVPNRSIGVFNLRGLTLFGLYVAGVVSAMAVAWLFKRFWIRSRYQPLMLELPPY
                     RAPGLRNLALGLWERAHIFLARVGGIIFSLMVVLWFLSSYPAPPVGATGPAIQYSIAG
                     WLGQALQHVFAPIGFNWQISIALVPGLAAREVAVGALGTVYSMSAAGSQVADALSPVI
                     AKGWSLATAYSLLAWYVFAPQCISTLAVVKRETNSWRYPLLMAAYLFVLAYAAAFVTY
                     RTTLWLGG"
     gene            6692..6961
                     /locus_tag="VNU48_11760"
     CDS             6692..6961
                     /locus_tag="VNU48_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI11996.1"
                     /translation="MQLLQNLIVALLVAGCAVYAAWALMPAAARRGIAGSLLKLPLPA
                     AFERKMRKAATVSSGCGCDGCDHAPAKTARAKSQVVTFHHRPTRR"
     gene            complement(6962..>7365)
                     /gene="ispH"
                     /locus_tag="VNU48_11765"
                     /gene_synonym="lytB"
     CDS             complement(6962..>7365)
                     /gene="ispH"
                     /locus_tag="VNU48_11765"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560871.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HWI11997.1"
                     /translation="SVREPKMQDICYATQNRQDAVKVLAPAVDVVVVVGSPTSSNSNR
                     LRELAQRLGTDAYMVDEPDDLRPEWFDGKLRVGLTAGASAPDVLVQQVIVRLRALGAV
                     SVRKLAGVEETTHFPLPMGLGDKAMGEAPGG"
BASE COUNT         1079 a         2549 c         2483 g         1254 t
ORIGIN      
        1 gtacatcgtg ctgcgcgccc tcggcgggcg ggaagggatc gacctggcaa agcgcttccg
       61 gcccgatctg attgcgctcg acctggagat gcccgaggtc aatggcttcg acgtcgtcga
      121 ggcactcaag ggcgacgcgg ccactgcgcg cattcccatt gtcgtggtca ccgccaagga
      181 cctgaccccg gccgaccgca gtcatctcaa cgggcacatc cttgagatcg tcggcaaggc
      241 ggagttcaac cacggccgat tcatcggcga agtgcagcgc gcgctggcca agccggcata
      301 gcctgccgcc caattggaac gccgcgggtt tggacaggct tcacccccct gttcgcggca
      361 tccgctcgag cgagcccggg tcaccatagg gccacggcgc atgtgcgccc tgggagccgg
      421 aagtgttctg taccaatcat tcgattccgc acgaggattg tcggcactgc atggcgacgg
      481 tcgcagccat gcgcccgacg gtgttcgccg agctcgcagc ggcagaaatg cgctccggca
      541 ccgaatgctg cagccgctgc gactacgtct actacgaggt cgccgccaaa tgcccgcgct
      601 gcgccgagcc gcacccgttc aatctcgacc cgctcgagca tgctgtgcgg gccacgcgcg
      661 cgactggagg agagggaggg attcgaaccc tcggaacctt gcggttcgcc tgatttcgag
      721 tcaggtacat tcgaccactc tgccacctct ccggtgcggg ccgcggctcc ggcgatccgg
      781 agggccctgc tcggcgggtc tgtgccgagc cgcgaattct agcctaggct ccggcggcct
      841 tcgttgacgt caggaccgca agaccgccga ggtaggggct cagcacctcg ggcacattga
      901 tcgagccgtc ggcgttctgg ccgttctcga gcaccgcgac cagcgtgcgc cccacggcaa
      961 ggcccgagcc gttcaaggtg tgcacgagtt cgttcttgcc ctgcgcattc ctgaagcgcg
     1021 cctgcatgcg gcgcgcctgg aaggcttcgc agttcgagca cgagctgatc tcgcggtagg
     1081 tggcctgcgc cggcacccag acctcgaggt cgtaggtctt ggcggcgccg aagcccatgt
     1141 cgccgctgct gagcagcatc acgcggtacg gcaggccgag cttgcgcaac acggcctcgg
     1201 catggccgac catctgctcg agcgcagcgt cgctctcgtc cgggtgcgtg atctgcacca
     1261 tctcgacctt gtcgaactga tgctggcgaa tcatgccgcg cgtgtcgcgc ccggcgctgc
     1321 cggcctcgga gcgaaagcag gggctgtgcg cggtcagctt gatcggcagt tcgtcggcgg
     1381 cgagcacgcg ctcgcgcacg ctgttcgtca gcgagatctc ggaggtcgag atcaggtact
     1441 gctcgggctg cgtctcgtcg ccgccgcggc tgacccagaa catgtcctcc ttgaacttgg
     1501 gcagttgccc ggtgccctcg aggatttcgc ggttcacgat gtacggcgtg tagcactcgg
     1561 tgtagccgtg ctcctgggtc tgcacgtcga gcatgaactg cgcgagcgcg cggtgcaggc
     1621 gcgcgaccgg gccgcgcagg aagctgaagc gcgagcccga gagcttggcg ccgaggtcga
     1681 agtccagccc gagcggcgcg ccgaggtcga catggtcgcg ggtcgggaag tcgaacaccc
     1741 gcggcgtgcc ccagcgccgc acctcgacgt tggcggtctc gtcgggaccg accggcacat
     1801 cgtcctgcgg caggttcggc agcgccatca gcagggccga cagctcggtc tggatcgagt
     1861 cgagccgctg cgccgaggcc ttgagcgtgt cgccgatgcc gccgacctcg gccatcgtcg
     1921 cggcgctgtc cccgccctgg gccttgatct ggccgatctg cttggacagg ctgttgcgcc
     1981 gcgcctgcag ctcctcggtg cggacctgga tcgtgcggcg ctccgcttcg agcgccgtga
     2041 agcggtcgac gtcgagaaac ggctgaggcg actggcgctt gtcgagccgg gcgacgaccg
     2101 aggcgagatc cttgcgcagc agagtgatgt cgagcatgtc gcggcgcgcg accggcgcgc
     2161 aggaggagtc gtggagcgcg gattgtaggc agcgcctgcg cggcgtggcg cgctgcgcgc
     2221 cgggtcgggc cgccctcagg ccatctgcgc ctgcaccggc cgggccgtcg tgatcacgtg
     2281 ctgctggtgc gtgccggtgc tgcgcgcgtc gcacagcacg cgcgaggcgc aatcggcgca
     2341 gcgcccgcac tgggtggcca cgccgaggtc gaactgcagg cactcgaggc tgtgcgcccc
     2401 gtcggccgcg gcctggcgga tctgatggtc agagactctg cgacacacgc aaacaatcat
     2461 gtccgtcctt tgctcgcgaa ccgcgcgccc gcgcgctagc tcgcctcgcc catctgcgac
     2521 tgcagatagt tttccaagcc caccttgccg atcagatcga tctgggtctc cagaaaatcg
     2581 atgtgctcct cggtatcgtc caggatgccc tgcaacacgt cgcgcgacac gtagtcgcgc
     2641 accgattcgc agtgcgcaat gccgtccttg atggtggcct gcgcgccggt ctcggactgc
     2701 aggtcgcagc tcagcagctc gggcacgttc tcgccgatca gcagcttgcc caggtcctgc
     2761 aggttcggca ggccgtcgag catgaagatc cgttccatca gccggtcggc atgcttcatc
     2821 tcgccgatcg actcttcgta ttccttcttc gccagcttgt ccaggcccca gtgcttgagc
     2881 atccggtagt gcaggaagta ctggttgacc gcggtcagct cgttcttcag ctgggcgttc
     2941 agatggacga gaacttgcgg gtcgcttttc atgggggctc cgatggctga ggtgtcggcg
     3001 cggcttcagc agcgcgcgcg gcctcgccgc gcgacagcgc ggcggcaggc tgcattgtga
     3061 aacgacatcg gcggcatcgg gtgatggaac ggcagactgg cgtgggtcaa cacggatgca
     3121 ttatgattgc aaatcgttcg cattagcaat ccgggtggaa tgcccctggt ttcgtgtgtt
     3181 caacaggggg ttctcgtgct ggtccgctgg tcgttcatgc tcggtatgtt cagtgcgctg
     3241 ctgctcggcg ggtgcggcgg tggcgggggt tcggcgcgtg acgcgagcgt gccggcggtc
     3301 gccgcgttgt cggcccaggc cgcgctgggc gagaagatct tcgccgacgt gtcgctgtcc
     3361 gcttcgggcc gccaggcctg tgcaagctgc catgaccccg acaacgcgcg cgcgccggcg
     3421 aacgacttgt cggtgcaact cggcggcccg ctgctcgacc agcaaggccg acggcaggcg
     3481 ccgtcgatcc gctacctgtc gttcaacacc gcgttcttct tcgcctcaga cggcacgccc
     3541 accgggggct tcttctggga cgggcgttcg ccctcgctgg cagagcaggc cggcgagccc
     3601 ttcgtcaacc cgttcgagat ggccaacgcg agcgtggacg aggtgatcga caggttgtct
     3661 cacgcgagct acgccgccga cttcaaggcc gtgttcggcg ccgacatctt cgaccgcccg
     3721 gtcgatgcga tggcgcgcgt ccaactggcc ctggcgcagt accagaagga ggacaccgag
     3781 ttccgcgcgt tctcgagcaa gtacgacgag ttcctgcgcg gcaacgtggc gctgtccgag
     3841 caggagctgc gcggcctggc gctgttcaac aacccgacga agggcaactg catcggctgc
     3901 cacccctcgg gcaagagcgc cgacggcgcg ttcccgctgt tcaccgactt cagctacgac
     3961 aacctcggcg taccgcgcaa cccgggcatc gcccgcaacg acgacccggg ctaccacgac
     4021 ctcggcctgt gcgaacgcag cgatctcgcg ggccgcgctg acctgtgcgg cgccttcaag
     4081 gtgccctcgc tgcgcaatgt cgcgcagcgc catgcgttgt tccacaacgg ccgcttcgca
     4141 acgctgaaag acgcgatcac cttctacgtg cagcgcgaca cgaacccgga gaagtggtac
     4201 ccgcgcaacc cggacggcag cgtgaacaag ttcgacgacc tgccggcgca gtaccgggtg
     4261 aacgtcaaca ccgccgaggt gccctacaac cgccgccccg gcgacgcgcc agccctgagc
     4321 gatgccgagg tcgacgacgt gatcgccttc ctggcgaccc tgaacgacgg ctacaggcgc
     4381 tgactctttg caccccactt gatcctgcgc aaacgcttcg ctcattgaga tcagggccaa
     4441 gtgtcgcggc cacggaactt cgcattcaaa tgagagacaa tctcatttgc atgtgcgtcc
     4501 cgatgcgcgt gcccttcccg taccaccatg ctgctgaacg aactgcccaa tggcgccttc
     4561 gccaccgtga cgcgtgtcgt ctcgacgtcg agcgacgtcg acgccgtggc gttgcggcgc
     4621 ctggccgaga tcggcttcat tcccggcgag ccggtgcagc tgctgcggcg cggcccgggc
     4681 gggcgcgagc cgctggcggt gctgatcggc gacacgatgt tcggcctgcg gctgctcgag
     4741 gcgcaatgcg tcgaagtccg cctcgaagtc cgggccgcct gagcctgcca tgtcgcaagc
     4801 cctgaacccg gcccacgcgc atgcaccctg gagcgtcgcg ctcgtcggca accccaattg
     4861 cggcaagacg gcgctgttca acctgctgac cggcgcgcgc cagaaggtcg ccaactacgc
     4921 cggcgtgacg gtcgagcgca aggtcggcac gacgcgcctg ccgggcgggc gcagcgtctc
     4981 ggtgatcgac ctgcccggcg cctacagcct gacgccggcc acgcccgacg agctggtcac
     5041 gctcgaggtg atcgaaggcc ggcgccgcgg tgaggacgca cccgacgcga tcgtcgccgt
     5101 ggtcgacgcg accaacctgc gcatgaacct gcgcctggtg ctcgagctca agcgcctgca
     5161 ccgcccgatg atggtggcgc tgaacatggc cgacatggcg cgcgcccagg gcctgaacat
     5221 cgatgtcgcg aagctcggcg ccgagctcgg ctgcccggtg gtcgagacga tcgcggtcag
     5281 gcacgacggc catgcgcagc tgctcgcgct gatggagcgc gagttcgccg cggcaccccc
     5341 cgaagcagtc gccggcagcg tcccgttcac gccgacatcg ccggccgagc tgcagcacga
     5401 agtgcggcgc atcctcgcga tcgtcgagcc cggcatcacg acccgggccg ccaacgcggc
     5461 gcgcttccat caccggctcg atgccgtcgt gatgcatccg gtgtgggggc tggcgatcct
     5521 cgcggtcgtg ctgttcctga tcttccaggc cgtgttctcg tgggccaacg tgccgatgga
     5581 cgcgatcaag gcggcgatgg cctggctcgg cgcgcaaacc accacgcaca tggccgaagg
     5641 gccgctgcgt tcgctgctgg tcgacggcgt gatcgccggc gtcggcagcg tgatcgtgtt
     5701 cctgccgcag atcctgatcc tgttcttctt catcctgatc ctcgaggact cgggctacct
     5761 gccgcgcgcc gcgttcctgc tcgacaacct gatgggcaag gtcgggctgt cggggcgcgc
     5821 gttcatcccg ctgctgtcga gcttcgcctg cgcgatcccc ggcatcatgg ccacgcgcac
     5881 gatcgccaac tggaaggacc ggctcgcgac catcatgatc gcgccgctga tgacctgctc
     5941 ggcgcgcctg ccggtctatg cgctgctgat cggcgcgttc gtgccgaacc gcagcatcgg
     6001 ggtgttcaac ctgcgcggcc tcacgctgtt cgggctgtac gtggccggcg tcgtctcggc
     6061 gatggcggtg gcttggctct tcaagcgctt ctggatcagg agccgctacc agccgctgat
     6121 gctcgagctc ccgccgtacc gcgcgccggg cctgcgcaat ctggcactcg ggctgtggga
     6181 acgtgcccac atcttcctgg cacgggtggg cggcatcatc ttctcgttga tggtggtgct
     6241 gtggttcctg tcgagctacc cggcaccacc cgtcggggcc accggcccgg cgatccagta
     6301 cagcatcgcc ggctggctcg gccaggcgct gcagcatgtg ttcgcgccga tcggcttcaa
     6361 ctggcagatc tcgatcgcgc tggtgccggg gctggcggcg cgcgaggtcg cggtcggtgc
     6421 gctcggcacg gtgtattcga tgtcggccgc cggcagccaa gtggccgatg cgctgtcgcc
     6481 ggtcatcgcc aagggctggt cgctggcgac cgcctattcg ctgctcgcct ggtatgtgtt
     6541 cgcgccgcaa tgcatctcga cgctggcggt cgtgaagcgc gagaccaact cgtggcgcta
     6601 cccgctgctg atggcggcct atctgttcgt gctcgcctac gccgcggcgt tcgtcaccta
     6661 ccgcacgacc ctctggctcg gaggctgaac catgcagctg ctgcagaacc tgatcgtcgc
     6721 gttgctggtc gcgggctgcg cggtctacgc ggcctgggcg ctgatgcccg ccgcggcgcg
     6781 gcgcggcata gccggctcgc tgctgaagct gccgctgccg gcagcgttcg aacggaagat
     6841 gcgcaaggcc gcgaccgtgt cgtcgggctg cggctgcgac ggctgcgacc acgcgcccgc
     6901 gaagaccgcg cgcgcgaagt cgcaggtggt gaccttccac caccggccga cacggcgctg
     6961 atcagccgcc gggcgcttcg cccatcgcct tgtcgcccag gcccatcggc agcgggaagt
     7021 gcgtcgtctc ctcgacgccc gcgagcttgc gcaccgacac cgcacccagc gcacgcagcc
     7081 gcacgatcac ctgctgcacc aggacgtcgg gcgccgaggc gcccgcggtc aggccgacgc
     7141 gcagcttgcc gtcgaaccac tccggccgca ggtcgtcggg ttcatcgacc atgtaggcat
     7201 cggtgcccag gcgctgcgcc agctcgcgca gccggttgct gttcgagctg gtcgggctgc
     7261 cgacgaccac caccacgtcg accgccggcg ccagcacctt gaccgcgtcc tgccggttct
     7321 gcgtggcgta gcagatgtcc tgcatcttcg gctcgcgcac gctcg
//
LOCUS       DATMJB010000550         2589 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_744232, whole genome shotgun sequence.
ACCESSION   DATMJB010000550 DATMJB010000000
VERSION     DATMJB010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_744232"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VNU48_11770"
     CDS             <1..791
                     /locus_tag="VNU48_11770"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWI11998.1"
                     /translation="LEMFRAPDKAALLRGLDQVFRDEMHAPFAEQLIDLWHGKLMQHR
                     EVVNYGLDGELINVLLQFSVLPGAETDWSLVQVALIDITAHKKAEAYLEYLGKHDVLT
                     QLRNRAFFEDELNRLERKGPWPVTIVAIDLNRLKATNDESGHAAGDALLRRAGEVLGK
                     VADKPVCAARIGGDEFVLVLPGEDERAGAAMIERVQSLVDLNNQYYPGATLSLAMGCA
                     TARPGERLEAAVSRADERMFEAKRDFYTSSGLDRRVTRPAPLAE"
     gene            complement(802..2451)
                     /locus_tag="VNU48_11775"
     CDS             complement(802..2451)
                     /locus_tag="VNU48_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI11999.1"
                     /translation="MTMRRRTLLAGALAAPLAACQRRTAPAWSGGWIGADAERGHRLR
                     DSKSGALPAPAVQRRADVIVVGGGIAGLAAARALAAAGIDNVQLLELEDMAGGNSRGH
                     VLGGMACPLGAHYLPVPGERAVEVIELLEALGLRRTERGVVVYDEMTLCHSPQERLRI
                     GGFWHDGLLPAIDALPAAQRDYTLAQYRRFGAAVAAAGADGAFAIPTARARWSPALDA
                     LDAQTFTAWLDAQRLDAPALRWYLDYCCRDDYGAGAAQVSAWAGLHYFASRHGFHAPG
                     DAADERDPVLTWPEGNAWLARKLAEPLRERLHTGVVVWRVDEGRDEVSVDLWNASAQR
                     SERWRAPQVVLALPLFVAARLLAAPPAALTQAAAALPHAPWLVGNLHLNDALIEHPGA
                     PLSWDNVVYEPGRENATLGYVDAMHQSTRALPGPTVLTAYWALGGDTRAQLVAQRARL
                     LAEPWSAWADAVVQDLARVHPDLRAKLRQVDLTRYGHAMCVPVPGLRSSAALRALAEP
                     QPRIQFAHSDLSAYSVFEEALYQGTRAGAAAARRLGTSRRR"
BASE COUNT          353 a          952 c          958 g          326 t
ORIGIN      
        1 cgctcgagat gttccgcgcg cccgacaagg ccgccttgct gcgcgggctc gaccaggtgt
       61 tccgcgacga gatgcacgcg ccgttcgccg agcagctgat cgacctgtgg cacggcaagc
      121 tgatgcagca ccgcgaggtc gtcaactacg gcctggacgg cgagctgatc aacgtgctgc
      181 tgcagttctc ggtgctgccc ggcgccgaga ccgactggag cctggtgcag gtcgcgctga
      241 tcgacatcac cgcgcacaag aaggccgagg cctacctcga gtacctgggc aagcacgacg
      301 tgctgacgca gctgcgcaac cgcgcgttct tcgaggacga actgaaccgc ctcgaacgca
      361 aggggccgtg gccggtgacg atcgtcgcga tcgacctgaa ccggctgaag gcgaccaacg
      421 acgagagcgg ccacgccgcc ggcgacgcgc tgctgcgccg cgccggcgag gtgctgggca
      481 aggtcgccga caagccggtg tgcgcggcgc gcatcggcgg cgacgagttc gtgctggtgc
      541 tgccgggcga ggacgagcgc gccggcgccg cgatgatcga gcgcgtgcag tcgctggtcg
      601 acctgaacaa ccagtactac cccggcgcga cgctgagcct ggcgatgggc tgcgccaccg
      661 cgcgccccgg cgagcggctc gaagcggcgg tgtcgcgcgc cgacgagcgc atgttcgagg
      721 ccaagcgcga tttctacacc tcgtcggggc tggaccggcg cgtcacgcgc ccggcaccgc
      781 tggcagagtg accgcagcgc gtcagcggcg gcgcgacgtg ccgagccgcc gcgccgcggc
      841 cgcaccggcg cgcgtgccct ggtacagcgc ttcctcgaac accgaatagg ccgacaggtc
      901 gctgtgcgcg aactggatgc gcggctgggg ttcggcgagc gcgcgcagcg cggcgctgct
      961 gcgcaggccc ggcaccggca cgcacatcgc gtggccgtag cgcgtcaggt cgacctgcct
     1021 cagtttcgcg cgcaggtccg ggtgcacgcg cgcgaggtcc tgcacgacgg cgtcggccca
     1081 cgccgaccac ggctcggcca gcagccgcgc gcgctgcgcg acgagttgcg cgcgcgtgtc
     1141 gccgccgagc gcccagtagg cggtcagcac ggtcgggccg ggcagcgcgc gcgtgctctg
     1201 gtgcatcgcg tcgacatagc ccagggtcgc gttctctcgc cccggctcgt agaccacgtt
     1261 gtcccacgac agcggcgcgc cggggtgctc gatcagcgcg tcgttcagat gcaggttgcc
     1321 gaccagccag ggcgcgtgcg gcagcgccgc ggcggcctgc gtcagcgccg ccggcggcgc
     1381 ggccagcagc cgcgcggcga cgaacagcgg cagcgccagc acgacctgcg gcgcgcgcca
     1441 gcgctcgctg cgctgcgcgc tcgcgttcca caggtcgacg ctgacctcgt cgcgcccttc
     1501 gtcgacgcgc cagacgacga caccggtgtg cagtcgctcg cgcagcggct cggccaactt
     1561 gcgggcgagc caggcattgc cttcgggcca ggtcagcacc gggtcgcgct cgtcggcggc
     1621 atcgccgggc gcgtggaagc cgtgccggct cgcgaagtag tgcagcccgg cccaggccga
     1681 gacctgcgcg gcgccggcgc cgtagtcgtc gcggcagcag tagtccaggt accagcgcag
     1741 cgccggcgcg tccaggcgct gcgcatcgag ccaggccgtg aaggtctgcg catcgagcgc
     1801 gtccagcgcc gggctccagc gcgcgcgcgc ggtcgggatc gcgaacgcgc cgtcggcacc
     1861 ggcggccgcg accgccgcgc cgaagcggcg gtactgcgcc agcgtgtagt cgcgctgcgc
     1921 ggcgggcagc gcatcgatcg ccggcagcag gccgtcgtgc cagaagccgc cgatgcgaag
     1981 ccgttcctgc gggctgtggc acagcgtcat ctcgtcgtag acgaccaccc cgcgctcggt
     2041 gcgacgcagg cccagcgctt cgagcagctc gatcacctcg accgcgcgct cgcccggcac
     2101 cggcaggtag tgcgcgccga gcgggcacgc catgccgccg agcacatggc cgcggctgtt
     2161 gccgccggcc atgtcttcga gctcgagcag ctgcacgttg tcgatgccgg cggctgccag
     2221 cgcacgcgcc gcggcaagcc cggcgatgcc gccgccgacg acgatcacgt cggcgcgccg
     2281 ctgcacggcg ggtgccggca acgcgcccga cttcgaatcg cgcaaccggt gcccgcgttc
     2341 ggcgtcggcg ccgatccagc cgccgctcca cgccggcgcc gtcctgcgct ggcacgccgc
     2401 gagcggcgcg gcgagcgcgc cggcaagcag cgtgcggcgg cgcatggtca tcaggcaacc
     2461 tccgcgccat gcgctccggt attcgccttg ggagcggccc ggcggctcat tggtgcacct
     2521 tgccccactc gtcttcgaag gtctggacga gcacctggtt ggagagtcgg ttgacgtcgg
     2581 cgggtacgc
//
LOCUS       DATMJB010000551         7312 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_259558, whole genome shotgun sequence.
ACCESSION   DATMJB010000551 DATMJB010000000
VERSION     DATMJB010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7312
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259558"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1437
                     /gene="fusA"
                     /locus_tag="VNU48_11780"
     CDS             <1..1437
                     /gene="fusA"
                     /locus_tag="VNU48_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWI12000.1"
                     /translation="FVERYLNDGDVDPRELHAPLEEALRQGHLIPICFVSARNGAGVG
                     ELLDVIEALLPNPAEGNPPQFYKGEGADAKELHANPDPAQHVIAHVFKITVDPYVGKM
                     GVFRIHQGTVTRDSQLYIGDGRKPFKVGHLFMLQGKEHVEVVRGVPGDVCAVAKIDEM
                     HFDAVLHDAAEDDHLHLKPLPFPVPVHGLAIEPKRRGDEQRMWEILQKLVDEDPCLKV
                     EHVGVTNETVVYGLGELHLRTLLERMTEVYKCEVNTRPPRIAYRETVTLNAEGHHRHK
                     KQSGGAGQFGEVFLRIEPLARGAGFEFVDQVKGGTIPTQFIPAVEKGIRQVLDTGVIA
                     GYPVKDVRVIVYDGKSHSVDSKDIAFATAGRKAFIDAVKKANPIVLEPIVKVEITAPE
                     HCMGDVTGDLAAKRGQVSGTRAGPGTGSVFIVTGQAPLSELANYQARLNGMTGGEGRY
                     TLELSHYEAVPPSAQAALMSQYRVKDED"
     gene            complement(1444..2676)
                     /gene="purT"
                     /locus_tag="VNU48_11785"
     CDS             complement(1444..2676)
                     /gene="purT"
                     /locus_tag="VNU48_11785"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HWI12001.1"
                     /translation="MTTLGTPLSPRATRVMLLGSGELGKEVLIALQRLGVETIAVDRY
                     ENAPGQQVAHHARTITMSDPAQLKALIEAERPMLVVPEIEAIATATLEELEAAGSVRV
                     IPTARAARLTMDREGIRRLAAETLALPTSPYCFCDSLAELQAAIEGVTQADGKRRPGI
                     GYPCVVKPVMSSSGKGQSKLDGPADVRAAWDHAMAGGRVSRGRVIVEGFIDFDYEITL
                     LTVRALGADGQVQTHFCEPIGHVQVAGDYVESWQPQPMHPVALERSRAIAKSVTDNLG
                     GQGLFGVELFVKGDAVWFSEVSPRPHDTGMVTMATQWQNEFELHARAILGLPVDTAMK
                     SPGASAVIYGGVEATGIVFDGVDAALRVPGTELRLFGKPESFVKRRMGVALAHDADVE
                     LARARAKQAAACVKPRAA"
     gene            2871..3461
                     /locus_tag="VNU48_11790"
     CDS             2871..3461
                     /locus_tag="VNU48_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12002.1"
                     /translation="MNPGPRDVAQIRLANALILFDEFVHATVKHADAATLRGLEGRFA
                     ERLQIQPSYWSQIKGRSRQIGERLARQFEQLCKKPAGWMDELHAPHDWAQAPQVGARV
                     PAAVLAAPGPGDDSQRSAPQDDDERFIVGLVLTYYRRHPQRARARLLDLLGEVLTPGP
                     ATAAKPGATRPAAPADDGRDLWSKTRAAVKPLKPKR"
     gene            3504..3722
                     /locus_tag="VNU48_11795"
     CDS             3504..3722
                     /locus_tag="VNU48_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12003.1"
                     /translation="MRKIIKRLSFAQVAGGFTHRDDTADPPSTKFMTRLNASIPAPAL
                     RIAPSVGASMPSRSVKGAQRAAPGSPPR"
     gene            3842..4003
                     /locus_tag="VNU48_11800"
     CDS             3842..4003
                     /locus_tag="VNU48_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12004.1"
                     /translation="MKITVHTRKPRNPIVAHARFRRAGSHQPGGRSQRQHAGRSLQRE
                     LEQMKQHGP"
     gene            4046..4309
                     /locus_tag="VNU48_11805"
     CDS             4046..4309
                     /locus_tag="VNU48_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12005.1"
                     /translation="MSSLKYLRAGLVEKPRPAREAALRIAAVGLRSASAALARLSRRL
                     ARPASRAPHPVPQLEFYAEAGAPEGALYLDGRLVGWVGGVQRL"
     gene            complement(4362..5213)
                     /locus_tag="VNU48_11810"
     CDS             complement(4362..5213)
                     /locus_tag="VNU48_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWI12006.1"
                     /translation="MNSVVLSAGVRLPALGLGTWHMGEAARTRAAEVAAVRLAIEIGY
                     RVVDTAEMYGEGGAEEVVGQAVAEALRSGAVTRDELFIVSKVYPHNASRAGVVAACDR
                     SLRRLALDSIDLYLLHWPGPHPLRDTVTGFEALRVDGRIRHWGVSNFDTAEMKALADA
                     GGARCAANQVYYSLSTRGAEFDLLPWQRERSMPLMAYCPLDQGGLVDEPVLREVAGRH
                     GASASQVALAWLMAQQGVMVIPKAVREAHLRENFAAADLVLDAADLSTIERRFPAPRR
                     KQALAMR"
     gene            5292..6389
                     /locus_tag="VNU48_11815"
     CDS             5292..6389
                     /locus_tag="VNU48_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2855 family protein"
                     /protein_id="HWI12007.1"
                     /translation="MTTTFVVERHKLNQTRWVHSEPAPLAQGDVRLAIASFALTSNNV
                     TYAAFGEAMNYWGFFPTGDAATGCIPVWGFADVTESRCDGVAIGERFYGYWPIANEVV
                     LQPTRLDAAGFSDGAAHRRELHAVYNRYLRCSADPGYDAAREAEQALLRPLFFTSFLI
                     DDFLADNDFFGARTVILSSASSKTAYGTAFCLAQRRGTTSAVRIVGLTSHANLGFTRG
                     LGCYDEVLDYDAVATLPVDAPAVYVDFSGSVALRAAVHGRFGDRLAYSCSVGGTHWDD
                     LGSGKGLPGPRPTLFFAPAQIKKRNADWGPAGLRQRLAAAWLAFMRPVTDARQPWLRV
                     VRGHGAAAIESTYAALLGGSANPAEGHVLSV"
     gene            6462..7307
                     /locus_tag="VNU48_11820"
     CDS             6462..7307
                     /locus_tag="VNU48_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWI12008.1"
                     /translation="MTLSANPASPQLTQPLRDLAAWTAHFRGAEIPVLAHTAEALEAL
                     RANEDDVDANSIGETIASDPLMTLKVLAYASVHRSARVVTDVETVTSALVMMGISPFF
                     AAFGPQPTVEERLAAEPLALQGLTDTLRRAHRGANFALGFAVHRMDHDAAVIHAAALL
                     HDFAEMLLWCHAPALALRIREAQHADPTLRSSVAQLAILNIELADLQQALMKAWRLPE
                     LLIRISDDRHADNASVRSVTLALRLARHSAKGWDNPAIPDDVSDIANLLNLSQVATLQ
                     LVREI"
BASE COUNT         1149 a         2641 c         2462 g         1060 t
ORIGIN      
        1 ttcgtcgagc gctacctgaa cgacggcgat gtcgacccgc gcgagttgca tgcgccgctc
       61 gaggaggcgc tgcgccaggg gcacctgatt ccgatctgct tcgtctcggc gcgcaacggc
      121 gccggcgtgg gcgagttgct cgacgtgatc gaggccttgc tgccgaaccc ggccgaaggc
      181 aacccgccgc agttctacaa gggcgagggc gcggacgcca aggaattgca tgccaacccc
      241 gatccggcgc agcatgtgat cgcgcatgtg ttcaagatca ccgtcgatcc atacgtcggc
      301 aagatgggcg tgttccgcat ccaccagggc acggtcacgc gcgacagcca gctctacatc
      361 ggcgacgggc gcaagccgtt caaggtcggc cacctgttca tgctgcaggg caaggagcac
      421 gtcgaggtcg tgcgcggcgt acccggcgac gtctgcgctg tcgcgaagat cgacgagatg
      481 cacttcgacg cggtgctgca cgacgccgcc gaagacgatc acctgcacct gaagccgctg
      541 cccttcccgg tgccggtgca cggcctggcg atcgagccca agcgccgcgg cgacgagcag
      601 cgcatgtggg agatcctgca gaagctggtc gacgaagacc cctgcctgaa ggtcgagcat
      661 gtcggcgtga ccaacgagac cgtggtctac ggcctgggcg agctgcacct gcgcacgctg
      721 ctcgaacgca tgaccgaggt ctacaagtgc gaggtcaaca cccggccacc gcgcatcgcc
      781 taccgcgaga ccgtgacgct gaatgccgag ggccaccatc gccacaagaa gcagagcggc
      841 ggcgcgggcc agttcggcga ggtgttcctg cgcatcgagc cgctggcgcg cggcgcgggc
      901 ttcgaattcg tcgaccaggt caagggcggc acgatcccga cccagttcat cccggcggtc
      961 gagaagggca tccgccaggt gctcgacacc ggcgtgatcg ccggttaccc ggtcaaggac
     1021 gtgcgcgtga tcgtctacga cggcaagtcg cactcggtcg attcgaagga catcgcgttc
     1081 gccaccgcgg gccgcaaggc cttcatcgac gcggtcaaga aggcgaaccc gatcgtgctc
     1141 gagccgatcg tgaaggtcga gatcaccgca cccgaacact gcatgggcga cgtgaccggc
     1201 gacctcgcgg ccaagcgcgg ccaggtcagc ggcacacgcg ccgggccggg aaccggcagc
     1261 gtgttcatcg tgacggggca ggcgccgctg tcggagcttg cgaactacca ggcgcgcctg
     1321 aacggcatga ccggcggcga gggccgctac acgctcgaac tcagtcacta cgaggccgtg
     1381 ccgccctcgg cgcaggcggc actgatgagc cagtaccgcg tcaaggacga agactgatcc
     1441 gggctacgcc gcccgcggct tgacgcaggc cgcggcctgc ttggcgcggg cccgcgcgag
     1501 ctcgacatcg gcgtcgtgcg cgagcgccac gcccatgcgc cgcttcacga agctctcggg
     1561 cttgccaaac aggcgcagct cggtgccggg cacgcgcagc gcggcatcga caccgtcgaa
     1621 cacgatgccc gttgcttcca cgccgccata gatcaccgcg ctcgcacccg ggctcttcat
     1681 cgcggtgtcg accggcaagc cgaggatcgc gcgcgcatgc agctcgaact cgttctgcca
     1741 ctgcgtggcc atcgtgacca tgccggtgtc gtgcgggcgc ggcgagacct cgctgaacca
     1801 gaccgcgtcg cccttgacga agagttcgac gccgaacagc ccctggccgc cgaggttgtc
     1861 ggtgacggac ttcgcgatcg cgcgcgagcg ttcgagggcg accgggtgca tcggctgcgg
     1921 ctgccagctc tcgacatagt cgccggcgac ctggacgtgg ccgatcggtt cgcagaagtg
     1981 cgtctgcacc tgaccatcgg cgccgagcgc gcgcacggtc agcagcgtga tctcgtagtc
     2041 gaaatcgatg aagccttcga cgatcacgcg gccgcggctg acacggccgc cggccatcgc
     2101 gtggtcccag gccgcgcgca cgtcggccgg gccgtcgagc ttgctctggc ccttgccgga
     2161 gctgctcatc acgggcttca cgacgcaggg gtagccgatc cccggccgac gcttgccgtc
     2221 ggcctgcgtg accccttcga tcgccgcctg cagttcggcc agcgagtcgc agaagcagta
     2281 cgggctggtc ggcagcgcga gcgtttcggc ggcgaggcgt cggatgccct cgcggtccat
     2341 cgtcaggcgc gcggcgcgcg ccgtcgggat cacgcgcacg gagccggcgg cctcgagttc
     2401 ctcgagcgtc gcggtggcga tcgcctcgat ctcgggcacg accagcatcg gccgctcggc
     2461 ctcgatcaat gccttgagct gcgccgggtc gctcatcgtg atcgtgcgtg cgtggtgcgc
     2521 gacctgctgc cccggcgcgt tctcgtagcg gtcgaccgcg atcgtctcga cgcccaggcg
     2581 ctgcagcgcg atcagcactt ccttgccgag ctcgccgctg cccagcagca tgacgcgggt
     2641 ggcgcggggc gagagcgggg ttccgagcgt ggtcatcgag cgcgtcctga agtgtgaagt
     2701 cgaggagcgc gaattctcgc cggaagcctg ggcctcgggg caacgccttc agcgcctcga
     2761 ccagcccggg gcagcaggtg ctgctgcagg aacgcgagca caaaggcggc cgccatcgtc
     2821 gttgaccccg ccccgcggcc cctgctcaac ggcgctttat catctgccac atgaaccccg
     2881 gcccgcgcga tgtcgcccag attcgcctcg cgaacgccct gatccttttc gacgagttcg
     2941 tccacgcgac cgtcaagcac gccgacgcgg cgacgctgcg cggcctcgaa ggccgattcg
     3001 ccgagcggct gcagatccag ccgagctact ggagccagat caaggggcgc tcgcgccaga
     3061 tcggcgagcg gctggcacgc cagttcgagc agctgtgcaa gaagcccgca ggctggatgg
     3121 acgagctgca cgcgccgcac gactgggcgc aggcgccgca ggtcggcgcg cgcgtgcccg
     3181 ccgccgtgct ggcagcgccc gggcccggcg acgacagcca gcgcagcgcc ccgcaggacg
     3241 acgacgagcg cttcatcgtc ggcctggtgc tgacctacta ccggcgccac ccgcagcgcg
     3301 cccgcgcgcg cctgctcgac ctgctcggcg aggtgctgac gcccggcccg gccacggcgg
     3361 ccaagcccgg tgcaacccgc ccggcggcac cggccgacga tgggcgcgac ctgtggagca
     3421 agacccgggc ggccgtgaaa ccgctcaagc ccaagcgctg atcttctttt gccacagggc
     3481 tcgagaattc tgcgcaatac cccttgcgca agataatcaa acgtttatca tttgcgcaag
     3541 tcgccggcgg attcacccat cgcgacgaca ccgccgatcc accttcaacg aagttcatga
     3601 cccgactcaa tgcatcgatt cctgccccgg ctctgcgcat cgcgccgagc gtcggtgcgt
     3661 cgatgccttc gcgatcggtc aaaggggcgc agcgcgcagc gccgggttca ccgccacgct
     3721 gaaacgtcgc acgcgacgct tcagtccgaa cggcccgggt cgcgcaaacg ccccgggccg
     3781 ttccgctttt tgcatcgtcg ctctcgcttc cgcttcgccc aacgccacca ggagaacgtt
     3841 catgaagatc accgtccaca cccgcaagcc gcgcaatccg atcgtcgcgc acgcccggtt
     3901 ccgccgcgcc ggcagccacc agcccggtgg ccgctcgcag cggcagcacg ccgggcgttc
     3961 gctgcagcgc gaactggaac agatgaagca gcacggcccc tgaatcacgc gcggcgaccc
     4021 cgcgccaccc tcgaaggaca gcaccatgtc gagcctgaaa tacctccgcg ccggccttgt
     4081 cgaaaagccc cgtcccgccc gtgaagccgc gttgcgcatc gccgcggtcg gactgcgcag
     4141 cgccagcgcc gcgctggccc gcctgtcgcg ccgcctggcg cgaccggcca gccgcgcgcc
     4201 tcacccggta ccgcagctgg agttctacgc cgaggcgggc gcccccgaag gcgccctgta
     4261 cctggacggt cgactcgtcg gctgggtcgg cggcgtgcag cgcctgtgag cgccggcggc
     4321 ggtgcacccg accttcggtg cgccgccttc tgttccattg atcagcgcat cgccagcgct
     4381 tgcttgcgcc gcggcgccgg aaatcggcgc tcgatcgtgc tgaggtcggc cgcgtcgagc
     4441 accagatcag cggccgcgaa attctcgcgc aagtgcgcct cgcgcaccgc tttcggaatg
     4501 accatcacgc cctgctgggc catcagccag gccagggcga cctgcgaagc gctggcgccg
     4561 tggcggcccg ccacttcacg cagcaccggc tcatccacca gcccaccctg gtcgagcggg
     4621 caataggcca tcagcggcat cgaacgttcg cgctgccacg gcagcagatc gaactcggca
     4681 ccgcgggtac tgagcgagta gtacacctga ttcgcggcgc agcgcgcgcc gccggcatcg
     4741 gccagcgcct tcatctcggc ggtgtcgaaa ttgctcacgc cccagtgccg gatgcgcccg
     4801 tccacgcgca aggcctcgaa gccggtgacg gtgtcgcgca gcgggtgcgg ccctggccag
     4861 tgcagcagat acaggtcgat cgaatcgagc gcgaggcgcc gcaggctgcg atcgcaggcc
     4921 gccaccacgc cggcgcggct ggcgttgtgc gggtagacct tgctgacgat gaagagctcg
     4981 tcgcgcgtga ccgcgccgct gcgcagggcc tcggccaccg cctggccgac gacctcttcg
     5041 gccccgccct cgccgtacat ctcggcggtg tcgaccacgc ggtagccgat ctcgatggcc
     5101 agccgcacgg cggcgacctc ggcagcgcgc gtgcgtgccg cctcgcccat gtgccaggtg
     5161 ccgagaccga gcgcgggcaa ccgaaccccg gcggataaca cgacgctgtt catgaggggc
     5221 tccggaaacg cgatactcga tgctaggcga gcgcgggcga cactgcgcgg caaacaccgg
     5281 agaccttcgc aatgaccacg accttcgtcg tcgagcgcca caagctgaac cagacgcgct
     5341 gggtgcacag cgagcccgcc ccgctggcgc agggcgatgt gcggctcgcg atcgccagct
     5401 ttgcgctgac atccaacaac gtcacctacg cggccttcgg cgaggcgatg aactactggg
     5461 ggttcttccc gacgggtgac gccgccaccg gctgcatccc ggtctggggg ttcgccgacg
     5521 tgaccgaatc gcgttgcgac ggggtggcga tcggcgagcg cttctatggc tactggccga
     5581 tcgcgaacga ggtggtgctg cagccgacgc gcctcgatgc cgccggcttc tcggacggcg
     5641 ccgcgcaccg ccgcgagctg cacgcggtct acaaccggta cctgcgctgc agcgccgatc
     5701 cgggctacga cgccgcgcgc gaggccgagc aggccttgct gcgcccattg ttcttcacct
     5761 cgttcctgat cgacgacttt ctcgccgaca acgacttctt cggcgcgcgc accgtgatcc
     5821 tgtcgagcgc gtccagcaag accgcctacg gcaccgcgtt ctgcctcgcg cagcggcgcg
     5881 gcacgacgag cgcggtgcgc atcgtcggct tgacctcgca cgccaacctg ggcttcacgc
     5941 gcggcctggg ctgctacgac gaggtgctgg actacgacgc cgtcgcgaca ctgcccgtcg
     6001 acgcgcccgc cgtgtacgtc gacttcagcg gcagcgtcgc gctgcgcgct gcggtccacg
     6061 gccggttcgg cgaccgcctg gcctacagct gttccgtcgg cggcacccac tgggacgatc
     6121 tgggcagcgg caagggcctg ccgggcccgc gtccgacact gttcttcgcg ccggcgcaga
     6181 tcaagaagcg caacgccgat tggggcccgg ccggcttgcg gcaacgtctt gcggccgcct
     6241 ggctcgcatt catgcggccg gtcaccgacg cccggcaacc gtggctgcgc gtggtgcgcg
     6301 ggcacggcgc ggcggcgatc gaatcgacct atgccgcttt gctggggggc agcgcgaacc
     6361 cggccgaagg gcatgtgctg tcggtctgag atggaccggc gctcaacccc cgcgcccgtg
     6421 ccgccgatat cctttcataa cgtcctgacg agcgagtcaa tatgaccctg tccgccaatc
     6481 cggcgagccc ccagctcacg cagccgctgc gcgatctggc ggcgtggacg gcgcacttcc
     6541 gcggcgccga gatcccggtg ctggcgcaca ccgccgaggc cctcgaggcc ttgcgcgcga
     6601 acgaagacga tgtcgacgcg aacagcatcg gcgagacgat cgccagcgat ccgctgatga
     6661 cgctgaaggt gctggcctat gcgtcggtgc accgcagtgc gcgcgtcgtc accgatgtcg
     6721 agacggtgac ctcggcgctg gtgatgatgg ggatctcgcc gttcttcgcc gcgttcggcc
     6781 cgcagccgac ggtcgaagag cggctcgccg ccgaaccgct ggcgctgcaa ggcctgaccg
     6841 acacgctgcg gcgcgcgcac cgcggcgcca acttcgcgct cggcttcgcg gtgcatcgca
     6901 tggaccacga tgccgcggtg atccatgccg ccgcgctgtt gcacgacttc gcagagatgc
     6961 tgctgtggtg ccacgcgccg gcgctggcgc tgcgcatccg cgaggcccag cacgccgatc
     7021 cgacgctgcg ttcgagcgtg gcccagctgg cgatcctcaa catcgagctc gccgacctgc
     7081 agcaggcgct gatgaaggcc tggcgcttgc cggagctgct gatccgcatc agcgacgatc
     7141 gccacgccga caacgccagc gtgcgcagcg tcacgctggc gctgcgcctg gcccggcaca
     7201 gcgcgaaggg ctgggacaac ccggcgattc ccgacgacgt gagcgacatc gcgaacctgc
     7261 tgaacctgtc gcaggtcgcg acgctgcagc tggtgcgcga gatctgatcc ga
//
LOCUS       DATMJB010000552         3947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_380668, whole genome shotgun sequence.
ACCESSION   DATMJB010000552 DATMJB010000000
VERSION     DATMJB010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380668"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..903
                     /locus_tag="VNU48_11825"
     CDS             7..903
                     /locus_tag="VNU48_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWI12009.1"
                     /translation="MNPFDRRQFLQLAGVGGAAVFISGLPRVAGAAAAKEEDFYFVQL
                     SDCHWGFEGAANPDARGTLPKAVASVNALTQGPDFIIFTGDLTHTTDDPKERRKRMAE
                     FRDIAGALQVKTVRFIPGEHDAALDNGKAFTEFFGPTHYTFEHKGVHFIVLDNVSDPG
                     AQLGDAQLAWLAADLKQQAKDARIVVFAHRPLFDLYPQWDWATRDGAKAVDLLMPYDN
                     VTVFYGHIHQEHHQMTGHIAHHSAKSLIFPLPVAGSQPKRTPLAWDAAQPYRGLGFRE
                     VEAEAKRVDYVITELPVQRARS"
     gene            900..1274
                     /locus_tag="VNU48_11830"
     CDS             900..1274
                     /locus_tag="VNU48_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWI12010.1"
                     /translation="MSVDSSRRTVIGAVGAIALGTIGVIAAAKAKPRVIKVVAKRFEF
                     VPAEIHVKQGETVTLQFSVVDVPMGFNLADFKLRADLMPGKPTLLELTPDRAGTFTFL
                     CDVFCGSGHEEMNGTLVVAPAA"
     gene            1378..2676
                     /locus_tag="VNU48_11835"
     CDS             1378..2676
                     /locus_tag="VNU48_11835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HWI12011.1"
                     /translation="MSHAVLDTLACPECELPQAVPALPPGGKAVCVRCKVTIARNPVD
                     PIDRPLALTVAAAVVFMVANLAPLMGLSVAGRESVTTILGGAQETWLRGSETTAVMVA
                     FCAVVAPGLFIAFMLVVLSAARRPPAPRWVGRLLRLAAFVEPWSMSEVMLLGILVALI
                     KIAQLATVTPGIGMYAVGLLVVLLAAIGSTFDSHAIWQRVVWADGTRPEPAPAAAPRR
                     RRLRALPNLADAGLASCTTCGLLSRPAPPGQQGRCPRCGTVLEAHAHASIQTTWALVI
                     AAAILYLPANALPVLVTTSLGSTESDTIISGVAYLYETGSWPLALIVLVASVIVPLGK
                     LIAIAYLLVTVQRGSVKNNHDRARLYRLVELVGRWSMLDVFVDAFIVALVQLSPLMSV
                     APGSGVAFFMTVVVLTMFAAHSFDQRLIWNARTVKRKQHG"
     gene            2669..>3947
                     /locus_tag="VNU48_11840"
     CDS             2669..>3947
                     /locus_tag="VNU48_11840"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWI12012.1"
                     /translation="MVEPSEGGELPQAMVVKPKRTRLSIVWLIPILAALVAIGIVVKR
                     VRSEGPTITIVFRAADGIEAGKTLIKYKDVNLGQVTSVHLSEDYSQVEVTAKITKRAA
                     GLMVEDAKFWVVRPTISLSGISGLNTLLSGNYIGFEAGTSPNEQTDFVGLDKAPVVSG
                     QLGREFILKARDLGSLSEGSPVYYRRLPVGQVVSYALTPDGKAVDVRVFVTAPYDKYV
                     TTGTRFWNASGIDIVAGANGFDIRTESLVSALVGGIAFDAPRLAPETASAPSDAEFTL
                     YADRPAAMKAPDPGARRYVLHFNETLHGLSVGAPVTFLGLTAGEVTGIDLEVDKSGRN
                     VSPRVTITFHPERRLAYADAAQQERMARILTGDDKQRNVLLQHLIDDRGLRAQLRSGS
                     LLTGQMYVAFEYFPKTGRSKIDWSRDAPDLPVVA"
BASE COUNT          598 a         1330 c         1374 g          645 t
ORIGIN      
        1 agagtgatga acccattcga tcgaagacag ttcttgcagt tggccggtgt cggaggtgcc
       61 gccgtgttca tctcgggcct gccgcgtgtt gccggcgccg cggcagcgaa ggaggaggat
      121 ttctacttcg tccaattgtc cgactgccac tggggcttcg agggtgctgc gaacccggat
      181 gcccgcggca ccttgccgaa ggccgtcgcg tcggtgaatg cattgacgca ggggcccgat
      241 ttcatcatct tcacgggcga cctgacccac accaccgacg atcccaagga gcggcgcaag
      301 cgcatggccg agtttcgcga catcgcgggc gcgctccagg tcaagaccgt gcgcttcatt
      361 cccggcgagc acgacgcggc actcgacaac ggcaaggcgt tcaccgaatt cttcggcccg
      421 acccactaca cgttcgagca caagggcgtg cacttcatcg tgctggacaa cgtcagcgat
      481 cccggcgcgc agctcggcga cgcgcagctc gcgtggctgg ccgccgacct gaagcagcag
      541 gccaaggacg cacgcatcgt cgtcttcgcg catcggccgc tgttcgatct gtacccgcag
      601 tgggactggg ccacgcgcga cggcgccaag gcggtggacc tgctgatgcc ctacgacaac
      661 gtgaccgtgt tctacggcca catccaccag gagcaccacc agatgaccgg gcacatcgcg
      721 caccactcgg cgaagtcgct gatcttcccg ctgccggtgg cgggctcgca gcccaagcgc
      781 acgccgctcg cgtgggacgc ggcgcaaccg tatcgcggat tgggtttccg cgaagtcgag
      841 gccgaggcca agcgggttga ctacgtcatc accgaactgc cggtgcagag ggcccgatca
      901 tgagcgtcga ctcgagcagg cgcaccgtga tcggtgcggt gggggcgatc gcgctcggca
      961 cgatcggggt catcgccgcg gcgaaggcca agccacgcgt catcaaggtc gtcgcgaagc
     1021 gcttcgagtt cgtgcccgcc gagatccacg tgaagcaggg cgagaccgtg accctgcagt
     1081 tcagcgtcgt cgacgtgccg atgggcttca acctcgccga cttcaagctg cgcgccgacc
     1141 tgatgccggg caagccgaca ctgctcgaac tcacgccgga ccgggccgga acgttcacgt
     1201 tcctgtgcga tgtcttctgc ggttcgggcc atgaggagat gaacggcacg ctggtcgtcg
     1261 cccccgcggc gtgacctgtt cggttatcgt ctagccccaa cagattgggg tgacgatgcg
     1321 tgtggttcga ctctgccgag ggacggccgc gactgcgccg acggcgtccc gcgctcgatg
     1381 agccacgcgg tgctcgacac gctggcctgc cccgaatgcg aattgccgca ggcggtgccc
     1441 gcgctgcctc caggcggcaa ggccgtgtgc gtgcggtgca aggtgacgat cgcgcgcaat
     1501 ccggtcgatc cgatcgaccg gccgctggcg ctgacggtgg ccgccgcggt ggtgttcatg
     1561 gtcgcgaacc tggcgccgct gatgggcctg tcggtggccg ggcgcgagtc ggtcacgacg
     1621 atcctcggcg gcgcgcagga gacctggctg cgcggcagcg agacgacggc cgtgatggtg
     1681 gcgttctgcg cggtggtcgc gccgggcctg ttcatcgcct tcatgctggt cgtgctgtcg
     1741 gcggcgcgcc ggccgccggc gccgcgctgg gtcggtcgcc tgctgcgcct ggcggcgttc
     1801 gtcgagccct ggtcgatgag cgaggtgatg ctgctgggca tcctggtcgc gctgatcaag
     1861 atcgcccagc tcgccaccgt gacgccgggc atcggcatgt acgcggtggg tctgctggtc
     1921 gtgctgctcg cggccatcgg gtcgaccttc gacagccacg cgatctggca gcgtgtcgtc
     1981 tgggccgacg gcacgcggcc cgagccggcg ccggcggccg cgccgcggcg ccggcgcctg
     2041 cgggcactgc cgaatctcgc ggacgccggg ctcgcgtcgt gcaccacctg cgggttgctc
     2101 tcgcggccgg cgccgcccgg gcagcagggc cggtgcccgc gctgcggcac ggtgctggaa
     2161 gcgcacgcgc acgcctcgat ccagaccacc tgggcgctgg tgatcgccgc cgccatcctg
     2221 tacctgccgg cgaatgcgct gccggtgctc gtgaccacct cgctggggtc gaccgagtcc
     2281 gacacgatca tctccggcgt cgcctacctg tacgagacgg ggtcgtggcc gctcgcgctg
     2341 atcgtgctgg tggccagcgt gatcgtgccc ttgggcaagc tgatcgcgat cgcctacctg
     2401 ctggtcacgg tgcagcgcgg ctcggtgaag aacaaccacg accgcgcacg gctgtatcgt
     2461 ctggtcgagc tggtcggccg gtggtcgatg ctcgatgtct tcgtcgatgc gttcatcgtc
     2521 gcgctggtgc agttgtcgcc gctgatgtcg gtggcacccg gctcgggcgt ggccttcttc
     2581 atgacggtcg tggtcctgac aatgtttgcc gcgcattcct tcgatcaacg cctgatctgg
     2641 aacgcgcgca ctgtgaaacg gaagcagcat ggttgaaccg agcgaaggcg gcgaactgcc
     2701 gcaggccatg gtcgtgaagc cgaagcgaac ccggctgtcg atcgtctggc tcatcccgat
     2761 cctggccgcg ctcgtggcca tcggcatcgt cgtgaaacgc gtccgcagcg aggggccgac
     2821 gatcacgatc gtgttccgcg ccgccgacgg catcgaggcc ggcaagacgc tcatcaagta
     2881 caaggatgtg aacctcggcc aggtgacgag cgtgcacctg tccgaggact acagccaggt
     2941 cgaggtcacc gcgaagatca cgaagcgggc cgccggcctg atggtcgagg acgcgaagtt
     3001 ctgggtcgtg cggccgacca tcagcctgag cggcatttcg gggctgaaca ccttgctgtc
     3061 gggcaactac atcggcttcg aggccggcac ttcgccgaac gagcagaccg atttcgtcgg
     3121 gctggacaag gcgccggtgg tctccggcca gctgggacgc gagttcatcc tgaaggcgcg
     3181 cgacctcggt tcgctgtccg agggatcgcc ggtctactac cgccgcctgc cggtggggca
     3241 ggtcgtgtcg tatgcgctga cgcccgacgg caaggcggtc gacgtccggg tgttcgtcac
     3301 cgcgccctac gacaagtacg tgacgaccgg cacgcgcttc tggaacgcga gcgggatcga
     3361 catcgtggcc ggcgcgaacg gcttcgacat ccgcaccgag tcgctcgtct cggcgctggt
     3421 cggcggcatc gcgttcgacg cgccgcggct cgcgcccgag acggccagtg ccccttccga
     3481 tgcggagttc acgctctacg ccgaccggcc ggccgcgatg aaggcgccgg accccggcgc
     3541 gcgtcgctac gtgctgcact tcaacgagac gctgcacggc ctgtcggtcg gcgcgccggt
     3601 caccttcctc ggcctgaccg ccggcgaggt caccggcatc gatctcgagg tcgacaagtc
     3661 cggcaggaac gtgagcccgc gcgtgaccat cacgttccac cccgaacgcc gcctggccta
     3721 cgccgatgcc gcgcagcagg aacggatggc gcgcatcctg accggtgacg acaagcagcg
     3781 caacgtgctg ctgcagcacc tgatcgacga ccgcggcctg cgcgcgcagc tgcgcagcgg
     3841 cagcctgctg acggggcaga tgtacgtggc gttcgaatac ttcccgaaga cgggcagaag
     3901 caagatcgac tggagccgcg acgcgccgga cctgccggtg gtggcca
//
LOCUS       DATMJB010000553         3611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_440591, whole genome shotgun sequence.
ACCESSION   DATMJB010000553 DATMJB010000000
VERSION     DATMJB010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440591"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VNU48_11845"
     CDS             <1..616
                     /locus_tag="VNU48_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HWI12013.1"
                     /translation="AVMGSALVAAVDGKDNPSVGLLNIGEEAIKGSETIKQAGELLRS
                     ASAHGMLNFYGNVEGNDIFKGTTDIVVCDGFVGNVALKTSEGLASMLSTFVKQEFTRN
                     VFTKLAAVVALPVLRHLQTRVDHRRYNGAALLGLRGLVFKSHGSADAFAFEHALNRAY
                     DAARNGLLDRVHDRILESLAAMPAPTVAAAVAGDPTAPDFVKTA"
     gene            613..1602
                     /locus_tag="VNU48_11850"
     CDS             613..1602
                     /locus_tag="VNU48_11850"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HWI12014.1"
                     /translation="MTSPTPRHTRITGTGSFLPPNRLTNNALAAQLAADGIETSDQWI
                     VERTGIRARHFAAPDVTSSDLAVEAARRALAAADVDAASIDLIIVATSTPDMVFPSTA
                     CLVQRKLGVAGCAAFDVQAVCSGFVYALTVADSMIRTGVASKALVIGAEVFSRILDFK
                     DRGTCVLFGDGAGAVVLEASATPGILASELHADGRHVDLLCVPGHVSGGQVLGDPLLR
                     MDGPAVFKIAVNVLYDVAKSVIEKAGRSSADVDWLIPHQANIRILQSTAKRLKLPLDK
                     LIVTVDEHGNTSAASIPLALDESVRSGKVARGDTVLLEGVGGGFTWGAVLLDL"
     gene            1667..2605
                     /gene="fabD"
                     /locus_tag="VNU48_11855"
     CDS             1667..2605
                     /gene="fabD"
                     /locus_tag="VNU48_11855"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HWI12015.1"
                     /translation="MTSFAFVFPGQGSQAVGMLDAWGDHPAVKRTLAEASEALGEDLA
                     QLIHAGPKDRLDLTTNTQPVMLTAGIACYRAWLAETQRAPSVVAGHSLGEYSALVAAG
                     ALTLADALPLVRFRAQAMQDAVPVGVGAMAAILGMDAQAVRDGCAEVAAATGEAVEAV
                     NFNDPKQTVIAGSKAGVDKACEALKAKGAKRALLLPVSAPFHSSLMRPAAERLKERLA
                     QVAFAAPAIAVINNIDVAIETEPGAIRNALYRQAFGPVRWVETVQAIRARGVTHIVEC
                     GPGKVLAGMVKRIDADAVSAALLDPATLADVKGLLA"
     gene            2602..3354
                     /gene="fabG"
                     /locus_tag="VNU48_11860"
     CDS             2602..3354
                     /gene="fabG"
                     /locus_tag="VNU48_11860"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HWI12016.1"
                     /translation="MTTEVHIAGQIALVTGASRGIGRAIALELAKQGMKVIGTATTDA
                     GAAAIGEALAAFPGSMGLTLNVNDGAGVDAAVDAIVKEHGALHVVVNNAGITRDTLSM
                     RMKDDDWDAVLDTNLKAVFRVSRAVTRPMMKQRYGRIINITSVVGASGNAGQANYAAA
                     KAGVAGMTRSLARELGSRGITVNCVAPGFIETDMTKALSEAQTTALMAQIPLGRLGHA
                     SDIAHAVAFLASPQAGYITGSELHVNGGMYMN"
     gene            3482..>3611
                     /locus_tag="VNU48_11865"
     CDS             3482..>3611
                     /locus_tag="VNU48_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWI12017.1"
                     /translation="MSDIEARVKKIIAEQLGVPEADVINNKAFVADLGADSLDTVEL"
BASE COUNT          587 a         1232 c         1263 g          529 t
ORIGIN      
        1 cgcagtgatg ggcagtgcgc tggtcgcggc cgtcgacggc aaggacaacc cgagcgtcgg
       61 cctgctgaac atcggggaag aggcgatcaa gggcagcgag acgatcaagc aggccggcga
      121 actgctgcgc tcggcatcgg ctcacggcat gctgaacttc tacggcaatg tcgaaggcaa
      181 cgacatcttc aagggcacga ccgacatcgt cgtgtgcgac ggcttcgtcg gcaacgttgc
      241 gctgaagacc tccgagggcc tggcgtcgat gctgtcgacc ttcgtcaagc aggaattcac
      301 gcgcaacgtc ttcaccaagc tggcggccgt cgtggcgctg ccggtgctgc gccatctgca
      361 gacgcgcgtc gaccaccggc gctacaacgg cgcggcgctg ctcggcctgc gcgggctggt
      421 cttcaagagc cacggttcgg ccgacgcgtt cgcgttcgag cacgcgctga atcgggctta
      481 tgatgccgct cgaaacggct tgctggaccg ggttcacgac cggatcctcg aatcgctggc
      541 cgccatgccc gcgccgacag tcgctgcggc ggttgcgggc gatccaaccg caccagactt
      601 cgtcaaaacg gcatgacatc gcccacgccc cgtcacacac gcatcaccgg caccggcagc
      661 ttcctgccgc cgaatcgcct gaccaacaac gcgctcgctg cgcagctggc ggccgacggc
      721 atcgagacct ccgaccagtg gatcgtcgag cgcaccggca tccgggcgcg ccatttcgcc
      781 gcgcccgatg tcacctccag cgacctggcg gtcgaagccg cgcggcgtgc gctggccgcg
      841 gccgacgtcg acgcggcgag catcgacctg atcatcgttg ccaccagcac gcccgacatg
      901 gtgttcccgt cgaccgcgtg cctggtgcaa cgcaagctcg gcgtcgccgg ctgcgccgcg
      961 ttcgacgtgc aggcggtgtg ctcgggcttc gtctatgcgt tgacggtggc cgattcgatg
     1021 atccgcaccg gggtggccag caaggcgctg gtgatcggcg ccgaggtgtt ctcgcgcatc
     1081 ctggacttca aggaccgcgg cacctgcgtg ctgttcggcg acggcgccgg cgcggtcgtg
     1141 ctcgaggcca gcgcgacgcc cggcatcctc gccagcgagt tgcacgccga cggtcggcac
     1201 gtggacctcc tgtgcgtgcc gggccacgtg tcgggtggcc aggtgctggg tgacccgctg
     1261 ctgaggatgg atgggccggc ggtgttcaag atcgccgtca acgtgctcta cgacgtcgcc
     1321 aaatcggtga tcgagaaggc cggccgcagc agcgccgatg tcgactggct gatcccgcac
     1381 caggccaaca tccgcatcct gcagagcacc gcgaagcgtc tgaagctgcc gctggacaag
     1441 ctcatcgtca ccgtcgacga gcacggcaac acctcggccg cgtcgatccc gctcgcgctc
     1501 gacgaatcgg tgcgcagcgg caaggtcgca cgcggcgaca ccgtgctgct cgagggcgtg
     1561 ggcggcggct tcacctgggg cgccgtgctg ctcgacctgt gaaccgcgcc ggcccgcctc
     1621 cccgctcacc tctgaccacc cgaccgcgct gcagcgccgc acattgatga ccagcttcgc
     1681 attcgtcttt cccggtcagg gctcgcaggc cgtgggcatg ctcgacgcct ggggcgatca
     1741 cccggccgtg aagcggaccc tggccgaagc atccgaggcg ctcggcgaag acctcgcgca
     1801 gctgatccac gccggcccga aggatcggct cgacctgacc accaacaccc agccggtgat
     1861 gctgaccgcc ggcatcgcct gctaccgcgc gtggctggcc gagacgcagc gcgccccgag
     1921 cgtcgtcgcc ggccactcgc tcggtgagta cagcgcgctg gtcgcggccg gcgcgctgac
     1981 cctggccgac gcgctgccac tggtgcgctt tcgcgcccag gcgatgcagg acgcggtgcc
     2041 ggtcggcgtc ggcgcgatgg cggcgatcct cggcatggac gcgcaggcgg tgcgcgacgg
     2101 ctgcgccgag gtcgccgcgg cgaccggcga ggcggtcgag gcggtgaact tcaacgaccc
     2161 gaagcagacc gtgatcgccg gcagcaaggc cggcgtcgac aaggcctgcg aggcattgaa
     2221 ggcgaagggt gcgaagcgcg cgctgttgct gccggtgtcg gcgccgttcc attcgagcct
     2281 gatgaggccg gcggccgagc ggctgaagga gcggctggcg caggtggcgt tcgcggcacc
     2341 ggcgatcgcc gtgatcaaca acatcgacgt cgcgatcgag accgagccgg gggcgatccg
     2401 caacgcgctc taccgtcagg ccttcgggcc ggtgcgctgg gtcgagacgg tgcaggcgat
     2461 ccgcgcgcgc ggcgtcacgc acatcgtcga atgcgggccc ggcaaggtgc tggccggcat
     2521 ggtcaagcgc atcgatgccg acgcggtcag cgccgcgttg ctcgatccgg caacgcttgc
     2581 cgacgtgaag gggctgctgg catgacgacc gaggtccata tcgcggggca gatcgcgctc
     2641 gtgaccggcg catcgcgcgg catcggccgg gcgatcgcgc tggagctggc gaagcagggc
     2701 atgaaggtga tcggcaccgc gaccaccgac gccggcgcgg cggcgatcgg cgaggcgctg
     2761 gcggcgttcc cgggcagcat gggcctgacc ctgaacgtca acgacggcgc tggtgtggac
     2821 gcggcggtcg acgcgatcgt caaagaacac ggcgcgctgc atgtcgtcgt caacaacgcc
     2881 ggcatcaccc gcgacacgct gtcgatgcgc atgaaggacg acgactggga cgcggtgctc
     2941 gacacgaatc tgaaggcggt gttccgcgtc agccgcgccg tgacccggcc gatgatgaag
     3001 cagcgctatg gccgcatcat caacatcacc tcggtcgtcg gtgcgagcgg caacgccggc
     3061 caggcgaact acgccgccgc gaaggcgggt gtggcgggca tgacccgctc gctggcgcgc
     3121 gaactcggca gccgcggcat caccgtcaac tgcgtcgccc ccggcttcat cgagaccgac
     3181 atgacgaagg cgctgagcga ggcgcagacg accgcgttga tggcgcagat cccgctcggc
     3241 cggctcggcc atgcgagcga catcgcgcac gcggtggcgt tcctcgcgtc gccgcaggcg
     3301 ggctacatca ccggcagcga gctgcacgtg aacggcggca tgtacatgaa ctgaggcgcg
     3361 ccgcgccgga aaattgtttg ccggtcgggc ccgaccggcc tgcggtcggt gtcccggaga
     3421 agggcgcctt gatggccgta gaatcgcggg ctgtttctgt aaacacactc ccggaggagt
     3481 catgagcgat atcgaagcac gtgtcaaaaa aatcattgct gagcaactcg gcgttcccga
     3541 agccgatgtg atcaacaaca aggccttcgt cgccgacctc ggtgccgatt ccctcgacac
     3601 ggtcgaactg g
//
LOCUS       DATMJB010000554         2250 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_555960, whole genome shotgun sequence.
ACCESSION   DATMJB010000554 DATMJB010000000
VERSION     DATMJB010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555960"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..452)
                     /locus_tag="VNU48_11870"
     CDS             complement(186..452)
                     /locus_tag="VNU48_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12018.1"
                     /translation="MTLQQFHELKLWHQRQGHRHPVERSLWDAVLTIWLLGWIGAPTA
                     FLLQLGWAELACASVLFLPGLYVALRRRLHNKRRLRCDWIIALK"
     gene            complement(537..1703)
                     /locus_tag="VNU48_11875"
     CDS             complement(537..1703)
                     /locus_tag="VNU48_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI12019.1"
                     /translation="MSTSALRRCGLALPPVRRVYAAFFLYAFAIGGIFPRLPEVQRTL
                     GVAEGAFGLALIGAACGTMVSLSLAGRALERVGHRRALLALVALLPLCYALASTARSA
                     PAFFVLLIPAGLCIGAVEIIVNLEADRVEHQTGRRFMNRAHAFWSVGFFAAGALGALL
                     SQLGVTPQAHLGAVVPLQALAVALLLGRFDAAPHRATTHSAAAARFARPTAGVMLLVA
                     LALSAMVLEGAGADWSAIYMRDVFGAGPFVAGIAVATGAATQAIARFFADRFVERHQP
                     VAVARVLLGVLGAGSLLVFVAPAGWAALLGFALMGVGTSAIFPLAMSAAAQRTDRAPA
                     TNVAALAQISFVAFLLGPPLLGYLAQALGMRWVFGAGLPLVALSFAASAALRVR"
     gene            complement(1700..>2250)
                     /locus_tag="VNU48_11880"
     CDS             complement(1700..>2250)
                     /locus_tag="VNU48_11880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012218155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HWI12020.1"
                     /translation="RHSFGNLVGHAKGVSDMSSLSAWTKEQFDPKLDWRDVEWIKKRW
                     GGKLILKGIMDVEDARLAADSGADALIVSNHGGRQLDGAPSSIAALPSIAQAVGRQIE
                     VWMDGGIRSGQDVLKAVALGAKGTLIGRSFLYGLGAMGEAGVTKALEIIAKELEITMA
                     FCGHTDIRTVDRDVLLPGTFPT"
BASE COUNT          376 a          816 c          805 g          253 t
ORIGIN      
        1 agtcgggtgg gcaacgcagc gaagtaaagg aggagggacg ggcgcagccc gttccggggg
       61 acatgagcgg agttgtccac ccgactctct cggctcgcgc cgaccgtcaa agggcgcacg
      121 ctcgcttgcg aacgaccgca aagggccgaa agccgacaca cggtcacagc gccacgcgac
      181 cagtttcatt tcagcgcgat gatccagtcg cagcgcagcc ggcgcttgtt gtgcaggcgc
      241 cggcgcagcg cgacgtacag gcccggcagg aacagcacgc tcgcgcaggc gagttcggcc
      301 cagccgagct gcagcaggaa agcggtcggg gcgccgatcc agccgagcag ccagatcgtc
      361 agcaccgcgt cccagagcga acgttcgacc gggtgccggt gcccctggcg ctggtgccag
      421 agcttgagtt cgtgaaactg ctgcagcgtc atcgaagcct ccgcggggtg gagccgatga
      481 accgactgta ggaccgcagc gccgccgtgc aaacacccag tttgcacagg ccgctgtcag
      541 cgcacgcgca atgccgcgct ggcggcgaag ctcagcgcca ccagcggcag cccggcgccg
      601 aacacccagc gcatgccgag ggcctgcgcc aggtagccca gcagcggcgg cccgagcagg
      661 aaggcgacga acgagatctg cgccagcgcc gcgacgttgg tggccggcgc gcgatcggtg
      721 cgctgcgccg ccgccgacat cgcgagcggg aagatcgcgc tcgtgcccac gcccatcagc
      781 gcgaagccga gcaaggcggc ccagccggcg ggcgcgacga acaccagcag gctgccggcg
      841 ccgagcacgc ccagcagcac gcgcgccacc gcgaccggct ggtggcgctc gacaaagcgg
      901 tcggcgaaga agcgcgcgat cgcctgcgtg gcggcgccgg tcgcgaccgc gatgccggcg
      961 acgaaggggc cggcgccgaa cacgtcgcgc atgtagatcg ccgaccagtc ggcgcccgcg
     1021 ccttcgagca ccatcgccga cagcgccagc gcgaccagca gcatcacgcc cgcggtcggc
     1081 cgcgcgaagc gcgccgctgc ggcgctgtgc gtggtggcgc gatgcggcgc ggcgtcgaag
     1141 cgcccgagca gcagcgcgac ggccagcgcc tgcagcggca cgacggcgcc caagtgcgcc
     1201 tgcggtgtca cgccgagctg cgacagcagc gcgcccagcg cgccggcggc gaagaagccg
     1261 acgctccaga acgcgtgcgc ccggttcatg aagcggcgcc cggtctggtg ctcgacccgg
     1321 tcggcctcga ggttgacgat gatctcgacc gcgccgatgc acagcccggc cgggatcagc
     1381 aacacgaaga acgcgggcgc gctgcgcgcc gtcgacgcga gcgcgtagca cagcggcagc
     1441 aacgcgacga gcgcgagcag cgcgcgccgg tggccgacgc gttcgagagc ccgccccgcc
     1501 aggctcagcg acaccatcgt gccgcaggcg gcgccgatca gcgccaggcc gaacgcgccc
     1561 tcggcgacgc ccagcgtgcg ctggacttcc ggcaggcgcg ggaagatgcc gccgatcgcg
     1621 aacgcgtaca ggaagaacgc cgcgtagacc cgccgcaccg gcggcaacgc gaggccgcag
     1681 cggcgcagcg ccgaggtgct caagtcggga acgtgcccgg cagcagcacg tcgcggtcga
     1741 cggtgcggat gtcggtgtgg ccgcagaacg ccatcgtgat ctcgagctcc ttggcgatga
     1801 tctcgagcgc cttggtgacg ccggcctcgc ccatcgcgcc cagcccgtac aggaacgagc
     1861 ggccgatcag ggtgcccttg gcgccgagcg cgaccgcctt cagcacgtcc tggccgctgc
     1921 ggatgccgcc gtccatccag acctcgatct ggcggccgac cgcctgcgcg atcgacggca
     1981 gcgcggcgat gctagacggc gcgccgtcga gctgacgccc gccgtggttc gagacgatca
     2041 gcgcgtcggc accgctgtcg gccgcgagcc gtgcgtcctc gacgtccatg atgcccttca
     2101 ggatcagctt gccgccccag cgcttcttga tccattcgac gtcgcgccag tcgagcttgg
     2161 gatcgaactg ctccttggtc caggcgctca acgagctcat gtcgctgacc cccttggcgt
     2221 ggccgaccag gttgccgaag ctgtggcgcc
//
LOCUS       DATMJB010000555         2133 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_365087, whole genome shotgun sequence.
ACCESSION   DATMJB010000555 DATMJB010000000
VERSION     DATMJB010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365087"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VNU48_11885"
     CDS             complement(<1..1039)
                     /locus_tag="VNU48_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12021.1"
                     /translation="MVAGEASARVEVRAAAQQALPWRVAGRRVAEPDRDVVQRRGRVQ
                     PGAQRGRVVGGEVFPQPGRRLRRVPGGGRGAERRPDRVQAMLGAAPPQPEVQQRLQAA
                     RPGARFAQRLDQPLARAQHTPGQQRADHEGEREQQRRRRRAEGALGGHRGQRHRSAGG
                     GQDRRAAPLRSARSRPDATDAAHADDCTERHRGPQAVPAQVPARSGVKFGAELPKSEQ
                     TLARPASPWPCAATPDGASPPCLTLPTMLLRLSRTSFWLAAAVAALALLAPGGHETLL
                     MLITGAASLLALGLWRSALRTELRRPAAALLSTPAPVLDRSGLRDAAAQIVSMAHDAP
                     SFEPALHAVARV"
     gene            1084..1728
                     /locus_tag="VNU48_11890"
     CDS             1084..1728
                     /locus_tag="VNU48_11890"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWI12022.1"
                     /translation="MPARPAAAPASPAADHESFSFTVSHDLRAPIRVVEGFTKIVKED
                     YGRLLDRVGNDHLDRVLGAAARMNNMIDALLALAKLSSQPLAQQPVNLSQLAGYVVDD
                     LRRQWPQRAVEIEIEPGMQVQGDPTLLRVVIENLLGNAWKYTGKAAQARIVFERDAQA
                     ADTFTVRDNGAGFDMRFADRLAERPLGGRSEATWGSTSITPARWQPAARAARRG"
     gene            complement(1679..>2133)
                     /locus_tag="VNU48_11895"
     CDS             complement(1679..>2133)
                     /locus_tag="VNU48_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWI12023.1"
                     /translation="SSFRHAIMMLGYQRLKRWLALLLATASKDVNLKPVMFAAVRRGL
                     LMEELVRSGGDEEMRNEMFICGVFSLLDRMFQQPFSQLLDSIPVPERVRQALAENAGP
                     YQPYVELVQAIEGEALFEFRDAADKLMMSVSEINRAVLRALLAATELE"
BASE COUNT          298 a          792 c          764 g          279 t
ORIGIN      
        1 gcacgcgggc gaccgcgtgc aaggccggct cgaaggacgg tgcgtcatgc gccatgctca
       61 cgatctgcgc cgccgcgtcg cgcaacccgg accggtccag caccggcgcc ggcgtcgaca
      121 gcagcgccgc cgcgggccgg cgcagctcgg tgcgcagggc cgaacgccac aggccgagcg
      181 ccagcaggct cgccgcgccg gtgatcagca tcagcagcgt ctcgtggccg cccggcgcca
      241 gcaacgccag tgccgccacc gcggcggcga gccagaagga cgtgcgcgac agtcggagca
      301 gcatggtcgg caaggtcagg catggcggac tggcaccatc aggcgttgcg gcgcagggcc
      361 acgggctcgc tggacgcgcg agggtctgct cggatttcgg cagttcggcg ccgaacttga
      421 cccccgagcg ggccggcacc tgcgcaggca ccgcctgggg cccccggtgg cgctccgtac
      481 aatcgtcggc atgagccgcg tcggtggcat caggacgact ccgcgccgac cggagcggcg
      541 cggcccgtcg gtcctggccg ccgcctgcgc tgcggtggcg ctgcccgcgg tggccgccga
      601 gagcgccctc ggcgcgtcga cgccgccgct gctgttcgcg ctcgccctcg tggtcggcgc
      661 gttgctggcc tggcgtgtgc tgcgcacgcg ccagcggctg gtcgagtcgc tgcgcgaagc
      721 gcgcgcccgg tcgggctgcc tggagccgtt gctggacctc tggctgtggc ggagcggccc
      781 cgagcatcgc ctggactcga tccggccgcc gctcggcgcc ccggccgccg cctggcacac
      841 gccgcagccg ccggcctggc tgtgggaaca cttcgccacc gacgacgcga ccgcgctgcg
      901 cgcccggttg aacgcggccg cgccgctgga cgacgtcgcg gtccggttcg gccacgcgac
      961 gcccggcgac gcgccacggc aaggcctgct gcgcggccgc gcgcacttcg acgcgggcgg
     1021 acgcttcgcc ggctaccacg gcaccttccg cgagctcgag gcgacgccgc cgcgggcccc
     1081 gcggtgcccg ctcggccggc ggccgccccg gcctcgccgg ccgccgatca cgagtcgttc
     1141 agcttcaccg tctcgcacga tctgcgcgcg ccgatccgcg tcgtcgaagg cttcaccaag
     1201 atcgtcaagg aggattacgg gcgcctgctc gaccgggtcg gcaacgacca cctggaccgc
     1261 gtgctcggcg ccgcagcgcg catgaacaac atgatcgacg cgctgctggc gctggccaag
     1321 ctgtcgtcgc agccgctcgc gcagcagccg gtgaacctgt cgcagctcgc cggttacgtc
     1381 gtcgacgacc tgcggcgcca gtggccgcag cgcgcggtgg agatcgagat cgagcccggc
     1441 atgcaggtgc agggcgaccc gacgctgctg cgcgtggtga tcgagaacct gctcggcaat
     1501 gcctggaagt acaccggcaa ggcggcccag gcccgcatcg tgttcgagcg cgatgcgcag
     1561 gccgccgaca ccttcacggt gcgcgacaac ggcgccggct tcgacatgcg cttcgccgac
     1621 cggctggctg agcgccccct cgggggcagg agcgaagcga cgtgggggtc gacatcaatc
     1681 actccagctc ggtggcagcc agcagcgcgc gcagcacggc gcggttgatc tcgctgacgc
     1741 tcatcatcag cttgtcggcc gcgtcgcgga actcgaacag cgcctcgcct tcgatcgcct
     1801 gcacaagctc gacatagggc tggtacggcc cggcgttctc ggccagcgcc tggcgcacgc
     1861 gttcgggcac cgggatcgaa tcgagcagct gcgagaacgg ctgctggaac atgcggtcga
     1921 gcagcgagaa cacgccgcag atgaacatct cgttgcgcat ttcctcgtcg ccgccgctgc
     1981 gcaccagctc ttccatcagc aggccgcggc gcaccgcggc gaacatcaca ggcttgaggt
     2041 tcacgtcctt gctcgcggtg gcgagcaaca gcgcgagcca gcgcttcagc cgctggtagc
     2101 ccagcatcat gatcgcgtgg cggaacgagc taa
//
LOCUS       DATMJB010000556         6447 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_628379, whole genome shotgun sequence.
ACCESSION   DATMJB010000556 DATMJB010000000
VERSION     DATMJB010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628379"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="VNU48_11900"
     CDS             complement(<1..172)
                     /locus_tag="VNU48_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWI12024.1"
                     /translation="MNRPTPAAAPALPHGIDAQTVFRSLFSAYPDAMLLVDQTGVIVL
                     ANPSAEQLLGYGA"
     gene            complement(177..914)
                     /locus_tag="VNU48_11905"
     CDS             complement(177..914)
                     /locus_tag="VNU48_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI12025.1"
                     /translation="MSSAAVHIAILDDEVDITLLLANYLQGHGFRVTQLHSGRALMAL
                     LPHDPPALVLLDLGLPGEDGFSIARQLREHWRCGLVIVTGRGDAVDKVVGLEVGADDY
                     VTKPFDLRELVARVKAVLRRMTPAPAAGSAASAAAPPRSLLRFAGWALDIGARQLLGP
                     QGAEVALTSGEFDLLCVFAKHPGRVLSRDFLLEQTRGREAGPFDRTVDVQVGRLRKKL
                     GDDGDDPKIIKSVRSAGYILVPTVTGP"
     gene            complement(1025..1867)
                     /locus_tag="VNU48_11910"
     CDS             complement(1025..1867)
                     /locus_tag="VNU48_11910"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWI12026.1"
                     /translation="MSYRSLLVLLDHHAACAARTACAVKLAKTLDCHLVGLAPTDLID
                     LPATPMAAASLTECAALVWDALREQAERAADRFRDACHAAGLRSFEAVIDEAGKAESL
                     VRHARCSDLTVLTQADPSAPDHAALQGLVESVVLHSARPTLVVPYAGRFDDIGSRVMV
                     AWDDSREAARAVSDALPLLQRAAQVEVVTWNEHAGVEAATLRPQLDALHQWLMWHGVS
                     AQLQVQTQVAGVAEAMLTHAADTNADLIVMGAYGHARWAERVLGGATRGALASMTVPV
                     LMSH"
     gene            complement(1903..2742)
                     /locus_tag="VNU48_11915"
     CDS             complement(1903..2742)
                     /locus_tag="VNU48_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWI12027.1"
                     /translation="MNTLRSILLHLDASPRSTRRLTLACELAAQHDGRVTALYASAPA
                     AFSVPFAMANGAADVLAMLQQLDIDYRDNAKALFDRSLSGTTPAVVWRELRNAAVIPG
                     VAAQALYTDLLVLGQHDPSDPLTVGIPPDFVASVLLASGKPALVLPYVDTCTTLGREV
                     LIAWKPARESAHAVAAAMPLLKRASRIHLTVATDPDALEAPARELEDCLRLHGVQAPV
                     QHHASLPDASAGDGLLSLAADVGADLLVMGCYGHSRARELVLGGASRTVLRSMTLPVL
                     MAH"
     gene            2928..4022
                     /locus_tag="VNU48_11920"
     CDS             2928..4022
                     /locus_tag="VNU48_11920"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWI12028.1"
                     /translation="MTTTTSDLATGPTRAAIPHRKVIRSWLAPISSRSTARALALVLL
                     DMALLVAGLAATVLARHPIVQVLAGAATGLVISRLFILGHDACHQSLTPHRALNRWLG
                     RIVFLPSLTPYSLWEVGHNVVHHGYTNLRGFDFVWQPLSLDEFRALPRWKRGLERVYR
                     SGWAPWLYYLIEIWWTRMFFPSRATMPTRRPVFARDCWLVSAFAIAWIAGLVAAASAT
                     QQSAAWLLTVGFALPFIVWNGMIGFVVYVHHTHLGVAWYEDKASWSASQPFVSTTVHL
                     TFRSAMGAVLHHIMEHTAHHVDMSVPLYRLKRAQALLECKLAGAIVVQPFSWRWYADT
                     ARRCKLYDFKARGWTDFAGRPSAQVETVHA"
     gene            complement(4256..4558)
                     /locus_tag="VNU48_11925"
     CDS             complement(4256..4558)
                     /locus_tag="VNU48_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HWI12029.1"
                     /translation="MTSRRQFIQIVPVVGGALLASRLAFAADVDPKDPQAVALGYVSD
                     ATKADKAKFPKYAAGQTCSNCALYQGKAGDASGPCPLFAGKTVAAKGWCSAYAKKA"
     gene            4781..4999
                     /locus_tag="VNU48_11930"
     CDS             4781..4999
                     /locus_tag="VNU48_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12030.1"
                     /translation="MNQHSSCHWWIGLFAGRLLQRQPRAHWFDVVQRAVAVYHRAAAL
                     DPLEAADRYSQSHELAVLGRSAQRPALR"
     gene            5122..>6447
                     /gene="zwf"
                     /locus_tag="VNU48_11935"
     CDS             5122..>6447
                     /gene="zwf"
                     /locus_tag="VNU48_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWI12031.1"
                     /translation="MTSDRVRHEAASDALVLFGATGDLARKRIFPALYAMCKRGALNL
                     PVIGVASSALSIEQFRARARESVERADAACDPGVLDRMLERLSYVGGRYEAAATFAAL
                     GRELGAARRPAYYLAIPPALFPTVIEGLDAAGLAAESRVIVEKPFGRDLASARELNRI
                     ALKVFAQDSIFRIDHFLGKEAIMNILYFRFANSFLEPIWNRHHVASVQITLAEQSGVE
                     GRGAFYESAGCLRDVVQNHLFQIVALLAMEPPASRGFEAVHRHQADVFDAMRPLRAGD
                     IVRGQYEGYRNEPQVANDSDVETYCAVRLHVDSWRWGGVPWYLRSGKHLPATACEVVV
                     RLKAPPQNLFADSSVREEANYLRFHLSPHSTIALGARVKRAGKEFVGDQREFSLLEEE
                     AGEQAPYERLLTDAMSGDGALFAREGAIEAAWAVVAPVLAEHPAVIPYAR"
BASE COUNT          954 a         2230 c         2313 g          950 t
ORIGIN      
        1 ccgcgccata gcccaggagc tgctcggcag acgggttggc cagcacgatg acgccggtct
       61 ggtccaccag caacatcgca tccggatagg cggagaacaa ggaccggaac accgtttgcg
      121 cgtcgatgcc atggggcagg gccggcgcgg cagcgggggt cggccggttc atcggttcag
      181 ggcccggtca ccgtcggcac gaggatgtag cctgcgcttc gaacggactt gatgatcttc
      241 ggatcgtcgc cgtcgtcgcc gagcttcttg cgcagccggc cgacctgcac gtcgacggtg
      301 cggtcgaagg ggccggcttc gcgaccgcgc gtctgctcga gcaggaagtc gcgcgacagc
      361 acccggcccg ggtgcttggc gaacacgcac agcagatcga actcgccgct ggtcagcgcc
      421 acttccgcgc cctgcgggcc gaggagctgc cgcgcaccga tgtcgagtgc ccagccggcg
      481 aagcgcagca ggctgcgcgg cggcgccgcg gcgctcgctg ccgaacccgc ggcgggggcc
      541 ggggtcatgc gccgcaacac ggccttgacg cgggccacga gttcacgcag gtcgaacggc
      601 ttggtcacgt agtcgtcggc accgacctcg aggccgacga ccttgtccac cgcgtcgccc
      661 cggccggtga cgatgaccag cccgcagcgc cagtgctcgc gcagctggcg ggcgatcgag
      721 aagccgtcct cacccggcag gccgaggtcg agcagcacca gcgcgggcgg gtcgtgcggc
      781 agcagcgcca tcagcgcgcg gccgctgtgc agctgcgtga cccggaagcc gtgcccctgc
      841 aggtagttgg ccagcagcag cgtgatgtcg acctcgtcgt cgaggatcgc gatgtggacg
      901 gcggcagagc tcacacgtgt cggcaggctg agggcagacg ccggcgaaac agccggccgg
      961 gcaagcgtat cagctttgcc ggtgcgcccc cggggccggc gaccgtgcag gcgcgccgcg
     1021 ttcgtcagtg cgacatcagc accggcacgg tcatcgatgc cagcgcgccg cgcgtcgcac
     1081 ccccgagcac gcgctcggcc cagcgcgcgt gcccgtaggc gcccatcacg atcaggtcgg
     1141 cgttcgtgtc cgcggcgtgc gtgagcatcg cctcggcgac gccggcaacc tgggtctgga
     1201 cctgcagctg cgcggaaacg ccatgccaca tcagccactg atgcagcgca tcgagctgcg
     1261 ggcgcaaggt cgcggcttcg acgccggcgt gctcgttcca ggtcacgacc tcgacctgcg
     1321 cggcgcgctg cagcaggggc agggcgtccg acacggcgcg ggcggcctcg cggctgtcgt
     1381 cccaggcgac catgacacgg ctgccgatgt cgtcgaagcg gcccgcatag ggcacgacca
     1441 gggtcgggcg cgcgctgtgc agcacgacgc tctcgaccag gccctggagg gcggcgtggt
     1501 cgggcgccga cgggtctgcc tgggtcagca cggtcaggtc gctgcaacgc gcgtgccgca
     1561 ccagcgactc ggccttgccg gcctcgtcga tcacggcttc gaacgaccgc aggccggccg
     1621 catggcatgc gtcgcgaaag cggtccgcgg cccgctcggc ctgctcgcgc agggcgtccc
     1681 acaccagcgc cgcgcactcg gtgagcgagg cggcggccat cggtgtggcc ggcaggtcga
     1741 tgaggtcggt cggcgcgagg ccgacgaggt ggcagtccag cgtcttggcg agcttcaccg
     1801 cgcacgcggt gcgcgcggcg caggccgcgt gatggtcgag caggacgagc aggctgcggt
     1861 agctcatggc ggtctccctg ggatggtgtc gatggtgtcg gctcagtgcg ccatcaatac
     1921 cggcagggtc atcgagcgca gcacggtgcg cgacgccccg ccgagcacga gttcgcgcgc
     1981 gcggctgtgc ccgtagcagc ccatgacgag caggtccgcg ccgacatcgg ccgccagcga
     2041 cagcaggccg tcgccggccg acgcgtcggg cagcgaggcg tggtgctgga ccggcgcctg
     2101 aacgccgtgc agtcgaaggc agtcctcgag ttcgcgtgcc ggcgcttcga gcgcgtccgg
     2161 atcggtggcg accgtcaggt ggatgcgcga tgcgcgcttc agaagcggca tcgccgccgc
     2221 gacggcgtgg gcggattcgc gcgccggctt ccacgcgatc aggacctctc gacccagggt
     2281 cgtgcaggtg tcgacgtaag gcagcacgag tgccggcttg ccgctggcca gcaggaccga
     2341 cgcgacgaag tccggcggga tcccgacggt cagggggtcg gacgggtcgt gctggccgag
     2401 caccagcagg tcggtgtaca gcgcctgggc ggcgacgccc gggatcaccg cggcgttgcg
     2461 cagctcgcgc cacacgaccg ccggcgtcgt gcccgagagc gagcgatcga acagcgcctt
     2521 cgcgttgtcg cgatagtcga tgtcgagctg ttgaagcatg gccagcacgt cggcggcgcc
     2581 attcgccatc gcgaacggca cgctgaacgc ggccggggcg ctggcataca gggcggtgac
     2641 acgcccgtcg tgctgcgcgg ccagctcgca ggccagggtc aggcgcctgg tgctgcgcgg
     2701 cgatgcgtcg aggtggagca ggatcgatcg aagggtgttc acggcggcgg cctcggaggc
     2761 ggtagtgctc gaatcgtgcc gcgggcgggg agccggacgc ttgcgttgga tcaagccgcg
     2821 cggcgcaggt gcacccatga caagtcattg atgcggcgca acatgggcgg gcgacgggtg
     2881 ggatgaaatc gcgtctcgac cggcatcacg caagggcaac gcgccacatg acaacgacga
     2941 ccagcgacct cgcgacgggc ccgacgcggg ccgccatccc gcaccggaag gtgattcgtt
     3001 cctggctcgc gccgatctcg tcgcgcagca cggcgcgtgc gctcgcgctg gtgctgctcg
     3061 acatggccct gctcgtcgcc gggctcgcgg ccacggtgct ggcgcgccac cccatcgttc
     3121 aagtgctggc gggcgccgcc acgggcctgg tgatcagccg gctgttcatc ctcggccacg
     3181 atgcctgcca ccagagcctg accccgcacc gcgccctgaa ccgctggctc gggcgcatcg
     3241 tcttcctgcc gtcgctgacg ccctacagcc tgtgggaagt cggccacaac gtcgtccacc
     3301 acggctacac caacctgcgc ggattcgact tcgtctggca gccgctgtcg ctcgacgagt
     3361 tccgcgcgct gccgcgatgg aagcgcgggc tcgagcgcgt ctaccgcagc ggctgggcgc
     3421 cgtggctcta ctacctgatc gagatctggt ggacgcgcat gttcttcccg agccgcgcga
     3481 cgatgccgac gcggcgcccc gtgttcgcgc gcgattgctg gctggtgtcg gccttcgcga
     3541 tcgcctggat cgccggtctc gtggccgcgg cctcggcgac gcagcaatcg gcggcatggc
     3601 tgctgacggt gggcttcgcg ctgccgttca tcgtctggaa cggcatgatc ggattcgtcg
     3661 tgtacgtgca ccacacgcac ctcggcgtcg cgtggtacga ggacaaggcc agctggtcgg
     3721 cctcgcagcc cttcgtctcg acgaccgtgc acctgacctt ccgctccgcc atgggcgcgg
     3781 tgctgcatca catcatggaa cacaccgcgc accacgtcga catgagcgtg cccttgtatc
     3841 gactcaagcg tgcgcaggcc ctgctcgaat gcaagctggc gggcgcgatc gtcgtccagc
     3901 cgttctcgtg gcgctggtat gccgatacgg cgcgccgctg caagctgtac gacttcaagg
     3961 caaggggatg gaccgacttc gccggccggc cgagcgcgca ggtcgagacc gttcacgcct
     4021 gacggtccac tcaccggtac gcacaaccat cgcttcaccg acgcagagga tcggcttcgc
     4081 agaacgcttc ggagattcga tccagcgcaa cgcggcgcgg gcggacttcg ctacgaaggc
     4141 gcgccggtgg cagcggccgg cggcgcgaac gccgccagca ccacaaggtg acccggcccg
     4201 aagcaggcct cggcgacgtc cgggacgcct cgggtcctgg cgcggccgac gcttctcagg
     4261 ccttcttcgc gtaggcgctg caccagccct tggcggcgac ggtcttgccg gcgaacagcg
     4321 ggcagggtcc gctggcatcg ccggccttgc cctggtacag cgcgcagttg ctgcaggtct
     4381 ggccggccgc gtacttcggg aacttggcct tgtcggcctt ggtcgcatcg gagacatagc
     4441 ccagcgccac agcctgcgga tccttcggat cgacgtcggc tgcgaaggcg agccggctcg
     4501 cgagcagcgc gccaccgacc acgggaacga tctggatgaa ttggcgacgt gaggtcatgc
     4561 gcagggtcct ttcagatgaa cggatgaagg ctggattgtc ggcgcacaag cccttgccgt
     4621 cggcagtgac tcgccgcgaa acgcacgccg cttgaccggc atcaagcccg ccggcgcgcg
     4681 ctaccgactg ttacggccag tcgcagcgca ccgtgcgctg ccgcacagac gcggcggacg
     4741 acgcccctcc agcatcagcg atggaccgag cgacgcacgc atgaaccagc acagctcctg
     4801 tcattggtgg atcggtctgt ttgccggacg actgctgcag cgccagccgc gcgcgcactg
     4861 gttcgacgtg gtgcaacggg ccgtggccgt ctatcaccgc gccgcggcgc tggacccgct
     4921 cgaggccgca gatcgttaca gccagtcgca tgagttggcg gttctgggtc ggtcggcgca
     4981 gcgcccggcg ctgcgctgac aggccggcgc gggcgcgcgc attgacaggc ctcgtcgtcg
     5041 cctcgaccat tcacgtcgat cagccgtgcg caggacgatg cgggttcgca tcgccttccg
     5101 cgatcgaaac gagggaccga catgacatcc gacagggttc gacacgaggc agcgtccgac
     5161 gcgctggtcc tgttcggcgc gaccggcgac ctggcgcgca agcggatctt tccggcgctc
     5221 tacgcgatgt gcaaacgcgg cgcgctgaac ctgccggtga tcggcgtggc gtcgtcggca
     5281 ttgagcatcg aacagttccg cgcccgggcc cgggagagcg tcgagcgcgc cgatgccgcc
     5341 tgcgacccag gcgtgctcga ccggatgctg gagcggctga gctacgtcgg cggccgctac
     5401 gaggcggcgg ccaccttcgc cgcgctcggg cgtgagctcg gcgcggcgcg gcgccccgcg
     5461 tactacctgg ccattccgcc cgcgctgttc ccgaccgtca tcgaaggtct agacgcggcc
     5521 ggtctcgccg ccgaaagccg cgtcatcgtc gagaagccct tcgggcgcga cctcgcatcg
     5581 gcgcgcgaac tcaaccgcat cgcactgaag gtgtttgcgc aagactcgat cttccgcatc
     5641 gatcacttcc tcggcaagga agcgatcatg aacatcctgt acttccgctt cgccaattcg
     5701 tttctcgaac cgatctggaa ccggcaccat gtcgcgagcg tgcagatcac gctggccgag
     5761 cagtccggcg tcgaggggcg cggcgcgttc tacgagagcg ccggctgcct gcgcgacgtg
     5821 gtgcagaacc acctgttcca gatcgtcgcg ctgctggcga tggagccgcc ggcgagccgc
     5881 ggcttcgagg ccgtgcaccg ccaccaggcc gacgtgttcg acgcgatgcg gccgctgcgc
     5941 gccggcgaca tcgtgcgcgg tcagtacgag ggctaccgca acgagcccca ggtcgcgaac
     6001 gattcggacg tcgagaccta ctgcgccgtg cggctgcatg tcgattcgtg gcgctggggc
     6061 ggcgtgccgt ggtacctgcg ttcgggcaag cacctgcccg cgaccgcgtg cgaggtggtc
     6121 gtgcgcttga aggcgccgcc acagaacctg ttcgccgact cgtcggtgcg cgaggaggcg
     6181 aactacctgc gctttcacct gtcgccccat tcgacgatcg cgctcggtgc ccgggtcaag
     6241 cgcgccggca aggagttcgt cggcgaccag cgcgagttca gcctgctgga agaggaagcc
     6301 ggcgagcagg cgccctacga gcgcttgctg accgacgcga tgagcggcga cggcgcgctg
     6361 ttcgcccgcg agggcgccat cgaggcggcg tgggccgtgg tggcgccggt gctggccgag
     6421 cacccggctg tgatcccgta tgcgcgg
//
LOCUS       DATMJB010000557         3147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_706588, whole genome shotgun sequence.
ACCESSION   DATMJB010000557 DATMJB010000000
VERSION     DATMJB010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706588"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..1667)
                     /locus_tag="VNU48_11940"
     CDS             complement(27..1667)
                     /locus_tag="VNU48_11940"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWI12032.1"
                     /translation="MSKLPEPPDTTAGAAAEQDLARLKERVESMRGVLVQLLQEVVLA
                     EKRLDDSQATRLLEANEQLVISVLEAQIDADTAAGVLGESTRLGGLDPLTELPNRTVL
                     LDRLAQAISHAKRYGHRLALLFLDLNDFKRINDTFGHAVGDRAIQLAADCLRSVVRET
                     DTVSRHGGDEFLMLLADITHAADAAAIAEKLNASLGSYSSVDDHAVRLSASIGISVYP
                     EDGEEATTLIDRADAAMYVAKRHEFGGFAFYSDPLAGQPRPEVPPVEPQQRRMTREQL
                     ALEESERRQAQLREANEQLVLAALGAQELRAAADSARQRQAELLTMVGQELSNPFAPI
                     RVAASMLGRQGADQVLLSRARALIEQQVNQMSRLVNGLLGPDDSRLKRGHRLFDLAAT
                     VADVARACQPAMDRRLQTLEIEIAAAPLEVRGDPVLIAQVLTNLLSNATSYSHSGGRV
                     QLSVEIVNHEVVMTVADNGAGIAPEALATIFDPFVRELRASAVNEEGLGIGLAVARAI
                     VEAHGGRVVARSAGIGHGSEFIVSLPMPQGCGAAPGGA"
     gene            complement(1692..>3147)
                     /locus_tag="VNU48_11945"
     CDS             complement(1692..>3147)
                     /locus_tag="VNU48_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HWI12033.1"
                     /translation="VPGFDAVLGGGLPEFSFNLIAGPPGCGKTTLAHQIMFALATPER
                     PALYFTVLGEPPLKMLRYQQQFEFFDTEALNQSIRFINLSDDAMAGDLDRVLERIVDE
                     VTQHGPALVFVDSFRSVVLASGNDAKPHNALQQFVQQLGMLMTSWQATTFLIGEYHVE
                     TDANPVFTVCDGLIWLRQSVQRNSMVRKMEVMKMRGQPTLPGLHTFRISPAGLEVFPP
                     ASAADPTRADAQAAMLAPRVLMGVPGLDEMLGGGLPRGYSLLVAGPSGSGKSILAAAF
                     LAEGARRGETGVIAAFEQHPNRSRSRVVADLIAAGHVGLVENRSADLSIDEVVFLLIG
                     EIRRLGATRVVIDSLSGFELALAPTFREDFRESLSSLVTALAGESVTVLMTSELEDRY
                     TDLRFSPYGTAFMTDAIIVQRYIEVDSRLHRMMAVVKVRASAHSDQLREFHIDADGIH
                     LDGMLTEQEGLLGGRPTRKVAGDLPGPRAGTSDA"
BASE COUNT          479 a         1096 c         1080 g          492 t
ORIGIN      
        1 ggtgtccgct gccgcgaggg gcggtttcag gcgcccccgg gggccgcacc gcagccctgc
       61 ggcatgggca gagacacgat gaattcactg ccgtgcccga taccggcact gcgcgcgaca
      121 acccgtcccc cgtgcgcctc gacgatcgct cgcgcgaccg cgagtccgat gcccaggccc
      181 tcctcgttga cggcgctggc ccgcagttcc ctgacgaacg gatcgaagat cgtcgccagc
      241 gcctcggggg cgatgccggc cccgttgtcg gcgacggtca tcaccacttc atggttcacg
      301 atctcgaccg acagctggac ccgcccgccg ctgtgggaat agctcgtcgc gttgctcagc
      361 aggttggtca acacctgtgc gatgaggacg gggtcgccac gcacttccag cggcgcggcg
      421 gcgatctcga tctccagggt ctgcaagcgc cggtccatcg cgggctggca ggcgcgcgcc
      481 acgtccgcga cggtggcggc caggtcgaac agtcggtgcc cgcgcttcag ccgactgtcg
      541 tcgggaccca gcaggccatt gaccaggcgc gacatctggt tcacctgctg ctcgatgagg
      601 gctcgagcgc gggacagcag cacctggtcg gcaccctgcc gacccagcat ggacgcggcg
      661 acgcgaatcg gcgcaaacgg gttgctcaac tcctgcccca ccatggtcag caactccgcc
      721 tgccgctgcc gtgcgctgtc cgccgcggcc cggagttcct gggcgccgag cgccgccagc
      781 accagctgtt cgttcgcctc gcgcagttgt gcctgcctgc gctcggactc ttccagcgcc
      841 agctgttcac gcgtcattcg ccgctgctgc ggctcgaccg ggggtacctc cgggcgcggc
      901 tggccggcaa gcgggtcgga atagaaggca aagccgccga actcgtgtcg cttggcgacg
      961 tacatcgcgg catccgcccg gtcgatcagc gtggtggctt cctcgccatc ttcgggatag
     1021 acgctgatgc cgatgctggc cgacagccgc accgcgtggt cgtcgacgct gctgtacgac
     1081 cccagcgacg cattcagttt ctcggcgatg gcggccgcgt cggccgcgtg cgtgatatcg
     1141 gccagcagca tgaggaactc gtcgccacca tgccggctga ccgtgtcggt ctcgcgcacc
     1201 acggatcgca ggcaatcggc cgcgagctgg atcgcccggt caccgaccgc gtggccgaag
     1261 gtgtcgttga tccgcttgaa gtcgttcagg tccaggaaca gcaatgccag ccggtggccg
     1321 tagcgcttcg cgtgggagat ggcctgcgcc agacggtcca gcagcaccgt gcggttgggc
     1381 agctcggtca gcggatcaag cccacccaag cgcgtcgact cgcccagcac accggcggcc
     1441 gtgtcggcat cgatctgcgc ctccagcacc gagatgacga gttgctcgtt ggcctcgagc
     1501 agccgggtcg cctggctgtc gtctaggcgc ttctcggcca ggacgacctc ctgcaacagc
     1561 tgcaccagca cgccgcgcat ggattccacg cgctctttca ggcgcgcgag atcctgctcg
     1621 gcggcggcac ccgccgtcgt gtcgggcggc tcggggagct tgctcatgct gccgacgccg
     1681 atccgcgaat ctcacgcatc ggaggtcccc gcgcgcgggc ctggcagatc ccccgcgacc
     1741 ttgcgggtcg gtctgccacc gagcaggcct tcctgctcgg tgagcatgcc gtccagatgg
     1801 atgccgtcgg cgtcgatgtg gaactcgcgc aactggtccg aatgcgcgct ggcgcgcacc
     1861 ttgaccacgg ccatcatgcg atgcaaccgg ctgtcgacct cgatgtagcg ctgcacgatg
     1921 atggcgtccg tcatgaaggc ggtgccatac gggctgaagc gcaggtcggt gtagcggtct
     1981 tcgagttccg aggtcatcag cacggtgacg ctttcgcccg ccagtgcggt gaccaggctc
     2041 gacagcgact cgcggaagtc ctcgcggaag gtcggtgcca gcgccagctc gaagcccgac
     2101 agcgaatcga tgacgacacg ggtggccccg aggcgccgga tctcgccgat cagaaggaac
     2161 acgacctcgt cgatcgacag gtcggcgctg cggttctcca ccaggccgac atggccggcg
     2221 gcgatcaggt cggcgacgac gcggctgcgc gaccgattcg ggtgctgctc gaaggccgcg
     2281 atgacacccg tctcgccgcg gcgcgcgccc tccgcgagga aggccgcagc caggatgctc
     2341 ttgcccgacc cggacgggcc ggcgaccagc agcgagtagc cgcgcggcag cccgccgccg
     2401 agcatctcgt ccagccccgg cacgcccatc agcacgcgcg gcgccagcat cgcggcctgc
     2461 gcgtcggcac gggtcggatc ggcggcgctg gcgggcggga acacctcgag gccggcgggg
     2521 ctgatgcgga aggtatgcag gcccggcagc gtcggctggc cgcgcatctt catgacctcc
     2581 atcttgcgca ccatggagtt gcgctgcacg ctctggcgca gccagatcag gccgtcgcac
     2641 accgtgaaca ccgggttggc gtcggtctcg acgtggtatt cgccgatcag gaaggtcgtg
     2701 gcctgccagc tcgtcatcag catgccgagc tgctgcacga actgctgcag ggcgttgtgc
     2761 ggtttggcat cgttgccgct ggcgagcacc accgagcgga acgaatcgac gaagaccagc
     2821 gccgggccgt gctgcgtgac ctcgtcgacg atgcgctcga gcacgcgatc caggtcgccg
     2881 gccatcgcgt cgtcggacag gttgatgaag cggatcgact ggttcagcgc ctcggtgtcg
     2941 aagaactcga actgctgctg gtagcgcagc atcttgagcg gcggttcgcc cagcacggtg
     3001 aaatacagcg cggggcgctc gggcgtggcc agcgcgaaca tgatctggtg cgcgagcgtc
     3061 gtcttgccgc agccgggcgg gccggcgatc aggttgaacg agaactccgg cagcccgccg
     3121 ccgagcacgg cgtcgaaccc cggcacg
//
LOCUS       DATMJB010000558         2547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_383143, whole genome shotgun sequence.
ACCESSION   DATMJB010000558 DATMJB010000000
VERSION     DATMJB010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383143"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VNU48_11950"
     CDS             <1..663
                     /locus_tag="VNU48_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI12034.1"
                     /translation="NDDYGKDYLKGLKDGLGDKVNLIVAEATYETSDATVDSQIVQLQ
                     GSGANVLFNIATPKFAAQAIRKAFDTGWKPTQYLANVSVSVATVLTPAGLDKSVGVLS
                     SIYGKDPTDTRWDNDPGMNEWRAFMKKYYPDGSLTDTFNVYGYSVARTLVQVLKQCGD
                     DLTRENVMRQAANLKDFGTGVALPGIRINTGPADYAPIESVQLVRFDGKTWTPVGEVM
                     GK"
     gene            complement(698..1405)
                     /locus_tag="VNU48_11955"
     CDS             complement(698..1405)
                     /locus_tag="VNU48_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI12035.1"
                     /translation="MAELLRVEGLRSGYGEAVVVQGVGLTLDEGQSLALLGRNGTGKT
                     TLLNTLVGVTRRHGGRIVLAGTDLTLLAPHQRAAAGIGWVPQERNIFKSLTVHENLGA
                     VARPGPWTVARAYEMFPRLAERKANMGNQLSGGEQQMLAVARALVLNPKLLLLDEPLE
                     GLAPIIVEELLRGIARVVRDEGMSAIIVEQNPRLILPITQRAVVLDRGAIVHEGTSAE
                     LLADRGTLDRWLAVARH"
     gene            complement(1398..2177)
                     /locus_tag="VNU48_11960"
     CDS             complement(1398..2177)
                     /locus_tag="VNU48_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI12036.1"
                     /translation="MSTVVLETRGLVKRFGGIVPTNDVSIQVERGARRALIGPNGAGK
                     TTLINLLTGVLEPSAGSILLDGVDITRLAPHQRVKRGIVRTFQINQLFNELTPLQSLA
                     LTVSAQLGVSAQWWKPLGGDARVAPICDTLLAQFRLDDVRDQQVKHLAYGKRRLLEIA
                     TALACAPRVLLLDEPVAGVPEGERQEIFDIVGALPAEVSVLLIEHDMDLVFNFASGVT
                     VLVNGAVFAQGSVDAISNDPRVKAVYLGEGEGEAMQEPAHG"
     gene            complement(2174..>2547)
                     /locus_tag="VNU48_11965"
     CDS             complement(2174..>2547)
                     /locus_tag="VNU48_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWI12037.1"
                     /translation="LRDNRLRAASIGLAVNRRLVAVYTIAAGVAGAAGTLLAQTSGFS
                     SLDVFDFHRSAEVLLVLVIGGTGYLYGGIFGAIVFKLLHDAVSAWTPQYWNFWLGIFL
                     VVLVMVGRETLIRPRTWFKHS"
BASE COUNT          412 a          895 c          884 g          356 t
ORIGIN      
        1 aacgacgact acggcaagga ctacctgaag ggcctgaagg acggcctggg cgacaaggtc
       61 aatctgatcg tggccgaggc cacttacgag accagcgacg ccaccgtgga ctcgcagatc
      121 gtgcagctgc aggggtcggg ggcgaacgtg ctgttcaaca tcgccacgcc gaagttcgcg
      181 gcccaggcga tccgcaaggc cttcgacacc ggctggaagc cgacgcagta cctggccaac
      241 gtgtcggtct cggtggccac cgtgctgacg ccggcgggcc tggacaagtc ggtgggcgtg
      301 ctgtcgtcga tctacggcaa ggacccgacc gacacgcgct gggacaacga ccccggcatg
      361 aacgagtggc gcgccttcat gaagaagtac tacccggacg gcagcctcac ggatacgttc
      421 aacgtgtacg gctacagcgt ggcccgcacc ctggtccagg tgctcaagca gtgcggcgac
      481 gacctgaccc gcgagaacgt gatgaggcag gccgcgaacc tgaaggactt cggcaccggc
      541 gtggcgctgc cgggcatccg gatcaacacc gggccggccg actacgcgcc gatcgagtcg
      601 gtgcagctgg tccggttcga cggcaagacc tggacgccgg tcggcgaggt gatgggcaag
      661 taggcggcgc gcgccgatcg cggcggcgcg tgaaagctca gtgccgcgcc accgcgagcc
      721 agcggtcgag cgtgccccgg tcggcgagca gctcggcgct cgtgccctcg tggacgatcg
      781 cgccgcggtc cagcaccacc gcgcgctgcg tgatcggcag gatcagccgg gggttctgtt
      841 cgacgatgat cgcgctcatg ccttcgtcgc gcaccacgcg cgcgatgccg cgcagcagct
      901 cctcgacgat gatcggcgcc aggccttcga gcggctcgtc gagcagcagc agcttcgggt
      961 tcagcaccag cgcacgcgcc accgccagca tctgctgctc gccgcccgag agctggttgc
     1021 ccatgttcgc cttgcgctcg gccagccgcg ggaacatctc gtaggcgcgc gccaccgtcc
     1081 acggccccgg gcgcgccacg gcgccgaggt tctcgtgcac cgtcagcgac ttgaagatgt
     1141 tgcgctcctg cggcacccag ccgatgccgg ccgcggcccg ttggtgcggc gcgagcagcg
     1201 tgaggtcggt gccggccagc acgatgcggc cgccgtggcg gcgcgtcacg ccgaccagcg
     1261 tgttcagcag cgttgtcttg ccggtgccgt tgcgcccgag cagcgcgagc gactggcctt
     1321 cgtcgagcgt gaggccgacc ccctgcacga cgacggcctc gccgtagccc gagcgcagcc
     1381 cttcgacgcg cagcagctca gccatgcgcc ggctcctgca tcgcttcgcc ttcgccttcg
     1441 ccgaggtaca ccgccttcac gcgcgggtcg ttcgagatcg cgtcgaccga gccctgcgcg
     1501 aacaccgccc cgttcaccag caccgtgacg ccgctcgcga aattgaacac caggtccatg
     1561 tcgtgctcga tcagcagcac cgagacctcg gccggcaagg cgcccacgat gtcgaagatc
     1621 tcctggcgct cgccctcggg cacgccggcc accggctcgt cgagcagcag cacgcgcggc
     1681 gcgcaggcca gcgcggtggc gatctcgagc aggcggcgct tgccgtaggc gaggtgcttg
     1741 acctgctggt cgcgcacgtc gtcgaggcgg aactgcgcca gcagcgtgtc gcagatcggc
     1801 gcgacacgcg cgtcgccgcc gagcggcttc caccactggg cactgacgcc cagctgcgcc
     1861 gagaccgtga gcgcgagcga ctgcagcggc gtcagctcgt tgaacagctg gttgatctgg
     1921 aaggtgcgca cgatgccgcg cttcacgcgc tggtgcggcg cgagccgggt gatgtcgacg
     1981 ccgtcgagaa gaatcgagcc ggcgctcggt tcgagcacgc cggtgagcag gttgatcagc
     2041 gtggtcttgc cggcgccgtt cgggccgatc agcgcgcgcc gcgcgccgcg ctcgacctgg
     2101 atcgacacgt cgttcgtcgg cacgatgccg ccgaagcgct tgaccaggcc gcgggtttcg
     2161 aggacgaccg tgctcatgag tgcttgaacc aggtgcgcgg ccggatcagc gtctcgcggc
     2221 ccaccatcac gagcacgacc aggaagatgc cgagccagaa gttccagtac tgcggggtcc
     2281 aggccgagac cgcatcgtgt agcagcttga acacgatcgc gccgaagatg ccgccataca
     2341 gatacccggt gccgccgatc accagcacca gcagcacctc ggcgctgcgg tggaagtcga
     2401 acacgtccag cgacgagaag ccgctggtct gcgcgagcag cgtgccggcc gcgccggcca
     2461 cgcccgcggc gatcgtgtag accgcgacca gccgccggtt gaccgcgagg ccgatgctcg
     2521 cggcacgcag ccggttgtcg cgcagcg
//
LOCUS       DATMJB010000559         2195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_214238, whole genome shotgun sequence.
ACCESSION   DATMJB010000559 DATMJB010000000
VERSION     DATMJB010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214238"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..144
                     /locus_tag="VNU48_11970"
     CDS             <1..144
                     /locus_tag="VNU48_11970"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWI12038.1"
                     /translation="QKRSSLIEVSEAGAQQLGPIAAELAYGEGLQAHARAAEMRLHRK
                     DEP"
     gene            165..1220
                     /gene="hisC"
                     /locus_tag="VNU48_11975"
     CDS             165..1220
                     /gene="hisC"
                     /locus_tag="VNU48_11975"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWI12039.1"
                     /translation="MKRVVRQDVQGMHGYAVQPSAGMVKVDTMENPFVLPEALRHALG
                     ARLAQVPLNRYPAERGDVLRAALATYAGMPEGCDIVLGNGSDELIAMLALACDVPGNV
                     VLAPLPGFVMYEMSAKLQGLKFVGVPTTADFELDLPLMLAAVREHRPALIYLAYPNNP
                     TANLWDDAAIDAIIEAAPGLVVMDEAYQPFAARDSMARLKRHEHVLLMRTMSKFGLAG
                     VRIGYLIGRSELIKEIDKLRPPFNISVLNCETALFALEHADEYARQAVLIRSERELLQ
                     MKLRDLPGVHAYPSDANMILVRVPDAKRVFEGMRARKVLIKNISGLHPLLANCVRITV
                     GTPEENILMVDALKASL"
     gene            1220..1831
                     /gene="hisB"
                     /locus_tag="VNU48_11980"
     CDS             1220..1831
                     /gene="hisB"
                     /locus_tag="VNU48_11980"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HWI12040.1"
                     /translation="MTIAEPNLQRRAEVVRHTNETKIRVALNLDGTGVASFATGIGFF
                     DHMLDQIARHALIDLEIEAQGDLHIDGHHTVEDVGITLGQAMAKAVGDKKGLRRYGHA
                     YVPLDEALSRVVIDFSGRPGLHMRVDFKSGMIGALDTQLVFEFFQGFSNHAGVTLHID
                     NLQGDNAHHQCETIFKAFARALRMALEIDPRAAGVIPSTKGSL"
BASE COUNT          382 a          716 c          743 g          354 t
ORIGIN      
        1 cagaagcgca gcagcctgat cgaggtcagc gaggccggcg cgcagcagct cgggccgatc
       61 gcggccgagc tcgcgtacgg cgaggggctg caggcgcacg cgcgcgcggc ggagatgcga
      121 ctccacagga aggatgaacc gtgagcgaga ccctgcagca gcggatgaag cgcgtggtgc
      181 gacaggacgt gcagggcatg cacggctacg cagtgcaacc ctcggccggc atggtcaagg
      241 tggacacgat ggagaacccg ttcgtgttgc cggaggcgct gcgccatgcg ctcggtgcgc
      301 gactcgcgca ggtgccgctg aatcgctacc cggccgagcg cggcgacgtg ctgcgcgcgg
      361 cactggccac ctacgccggc atgcccgaag gctgcgacat cgtgctcggc aacggctcgg
      421 acgagctgat cgcgatgctc gcgctggcgt gcgacgtacc aggcaatgtg gtgctcgcac
      481 cgctgcccgg cttcgtgatg tacgagatgt cggccaaact gcagggcctg aagttcgtcg
      541 gcgtgccgac aacggccgac ttcgagctcg acctgccttt gatgctcgcc gcggtgcgcg
      601 aacaccggcc ggcgctgatc tacctcgcgt acccgaacaa cccgactgcg aacctctggg
      661 acgatgccgc gatcgacgcg atcatcgagg ccgcgcctgg cctggtcgtg atggacgagg
      721 cgtatcagcc gttcgccgca cgcgattcga tggcgcgtct gaaacggcac gagcatgtgc
      781 tgctgatgcg caccatgagc aagttcggcc tggccggggt gcgcatcggc tacctgatcg
      841 gccgcagcga actgatcaag gagatcgaca agctgcgccc gccattcaac atcagcgtgc
      901 tcaattgcga gacagcgctg ttcgcactgg aacacgccga cgaatatgcg cgccaggccg
      961 tgctgatccg cagcgagcgc gagctgctgc agatgaagct gcgcgatttg ccgggcgtgc
     1021 atgcctatcc gagcgacgcg aacatgatcc tcgtgcgcgt gcccgatgcg aagcgcgtgt
     1081 tcgagggcat gcgtgcgcgc aaggtgctga tcaagaacat ttcgggcctg cacccgctgc
     1141 tcgcgaactg cgtgcgtatc acggttggca cgccggaaga gaatatcttg atggtcgatg
     1201 cgctgaaagc gagcctgtga tgacgatcgc cgaaccgaat ctccaacgtc gcgcggaagt
     1261 ggtccgccac accaacgaga cgaagatccg cgtcgcgctg aacctcgatg gaaccggcgt
     1321 cgcgagcttc gccaccggca tcggcttctt cgaccacatg ctcgaccaga tcgcgcgcca
     1381 tgcgctgatc gacctggaga tcgaggcgca gggcgatctg cacatcgacg gccatcacac
     1441 cgtcgaggat gtcggcatca cgctcggtca ggcgatggcc aaggcggtgg gcgataagaa
     1501 ggggctgcgt cgctacggcc acgcctacgt gccgctggac gaggcgctgt cgcgcgtcgt
     1561 catcgacttt tccggccggc cggggctgca catgcgcgtc gacttcaaga gcggcatgat
     1621 cggcgcgctc gacacgcagc tggtcttcga gttcttccag ggcttctcga accacgccgg
     1681 cgtgaccctg cacatcgaca acctgcaggg cgacaacgcg caccatcagt gcgagacgat
     1741 cttcaaggcc ttcgcgcgcg cgctgcgcat ggcgctcgag atcgatccgc gcgcggccgg
     1801 ggtgattccg tcgaccaagg ggagcctcta ggtgggttcg atcgtgctcg atgaaccgtc
     1861 gcagaggcca tggtcggctt tcggcccttt gcagacgctc gaacttggtg tgcgtccgtc
     1921 ggcgcggcaa aggcgcgccc gggcaggctg ctccgcttca ctgcggcggt cgtcgctgcg
     1981 cgccgactgc cctgcgctgc tcggtctcgt ggcccgtcgc caaactcact tcgctcactg
     2041 cgttcgctgc gttcaaacag acggcgacaa gtcagttcac gaggcgcgct acgcgcgcgg
     2101 gccacgagcc ctgcgctgct cggcgccgca gaggcgctgc gcaacctgcc cgggcacgcc
     2161 tttgctgcaa caccggtggt attcgggcga agaac
//
LOCUS       DATMJB010000560         6470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_793586, whole genome shotgun sequence.
ACCESSION   DATMJB010000560 DATMJB010000000
VERSION     DATMJB010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793586"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..989
                     /locus_tag="VNU48_11985"
     CDS             102..989
                     /locus_tag="VNU48_11985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWI12041.1"
                     /translation="MRKVGVVGWALAAPALVVIFVFFALPVAAGLLLSLTDFDLYALA
                     DLSTLRFVGLQNYARLLQTPLFWQALGNTFYFVLVGVPASIGLSLGAALLLNARVAKW
                     QPLFRTALFAPVVTTVVAVAVIWRYLLHTRYGLINQGLATLGVSPVDWLGNPHWSMPA
                     IILFAAWKNFGYNMIILLAALQAVPRELYESARVDGAGTLRQFVDLTFPMLTPTLVMV
                     SIMTISGYFQLFAEPYVMTQGGPLRSTVSVLYFMYEEGFRWWNLGNASAVAFLLFVVI
                     FAVSWVLLRFDRDPSGATA"
     gene            986..1807
                     /locus_tag="VNU48_11990"
     CDS             986..1807
                     /locus_tag="VNU48_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWI12042.1"
                     /translation="MNARWAVWVVNGLLAGLAFVSLFPLLWMLSVSFMPAGASSTLPT
                     PILPSRLTLDNYRQLFGSIGMGRYMLNSLALAVLSTVISVSFNVMAGYAFAKLRFRGR
                     DRVFKTLLGALVIPGQVAMLPLFLMLKPLGLINSYGGAVVPMMASVFGIFLVRQFARG
                     IPDDLLEAARIDGAHEFRIFLTIVVPLLTPVLATLAIFSFLGSWNDFMWPLIVLTDDS
                     LHTLPVALAGLSREHVQDNELMMAGSVVTVLPVLVLFLALQRHYMAGLMAGGVKG"
     gene            1804..4887
                     /locus_tag="VNU48_11995"
     CDS             1804..4887
                     /locus_tag="VNU48_11995"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HWI12043.1"
                     /translation="MTPPAWAAIALVVIAAAALPAQAQERVLDDFADPSAWTVSATDD
                     VKASLRAADGARGRALCIDYDFGRVTGYVTAQRALPIEFPARYEFTLGLRGQTLPNAF
                     QFKLVDASGANVWWGQRPEFRFPAQWQTLKFRQRQIEFAWGPIADRTLRRSASVELVI
                     ASGSGAGKGSACFDRLTLRELPPEAPPLPPPKMTADALGVDFGAPREFGALALRWRAG
                     AAARYAIELSNDGTQWQRVREVERARGELQRQWLPESEARFVRIVPQPSAGPPVALAA
                     IEPLDAPSANLFFAQLAQAAPRGAYPRAYRGEQSYWTVLGVDGGRTASLLSEDGVLEA
                     LPGIGALEPFLVVDGHVLNWADARITHSLRDGDLPMPAVHWTIGDLTLDVEAFGAGTP
                     EAAQALARYTLHNAGTRTQAVTLALAWRPFQANPPTQFLAHPGGASEIASIAWDGRAL
                     AVDGVVRLQPLTAPSGVQVEPLAAGPVGDWLRDGAASVAPQRVADAARFASGVLRYEW
                     RLAPGERREVAIALPVSGTPLAPTMDQFAHEQQRVAAHWRERLDRVRISGPHEVDDIA
                     RTLRSALGHILVNRSGPALQPGARAYARSWIRDGALTSSALLRLGHADEARDFLRWFA
                     PFQFKNGKVPCCATARGADPVPENDSDGEFAFAVADHWHYTRDEASARALWPHVRAAV
                     AHMEGLRQSERTEANRTPERAAYFGLMPPSISHEGYSDKAAYSYWDDFWAAIGYRSAV
                     ELAQALGLPDDAKRIAAQRDEFLVELRASIDASTRHFGLDVLPGAADRGDVDPTSSTI
                     ALSPGGLLATPPSATVRNTFERYWRDFEARRADEAAGVRHGDGGYTPYEWRNVGALVR
                     LGERERALQALAYFYRDRRPAGWNQWAEVVLRDPREPRFLGDMPHGWVASDQIRSVLD
                     LFAYEDEGTHALVLAAGVPLRWFDGTGLRVQGLRTPYGALSWQAHTHAASGRQRIDFE
                     LRPLRGWPAGGVELRGPWPADARVWVDGRAWAGPADAIRLSVTPARVRIEFASTE"
     gene            4964..5386
                     /locus_tag="VNU48_12000"
     CDS             4964..5386
                     /locus_tag="VNU48_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12044.1"
                     /translation="MPRRALIALVLASSLLAGCDMLVGALGIESPEKVAAAREAEGKA
                     IGGACRNAARAIEDCFALNRKADKAAVFAGWREMNDYMRENKIDPVTPQVVTKVAGAK
                     AATEAEEAVEPRAATTKPAAKTDAAKPRAHEDAKPHDS"
     gene            5461..6198
                     /locus_tag="VNU48_12005"
     CDS             5461..6198
                     /locus_tag="VNU48_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HWI12045.1"
                     /translation="MSDVLARPIWRHPEFARGSRDMIGVSLGIAAWGLVTGVAMAKSG
                     LPLPISILMSLLVFAGSAQLAALPLIVSGAPLWTVWAIALCVNLRFVIFSAQWRPYFA
                     HLPRWQRMLRGYFTADLNYVVFMARFPQPRPAPEQEPYFWGGVVTNWPSWQLPTLAGL
                     LLGDAIPTEWGFGFAGILALLGLMYSLLGDRASWVAAGVAGAAAVATAALPLKLNVVV
                     AIAAAVAIGLLMDHVTPAEAKPVEDNT"
     gene            6195..>6470
                     /locus_tag="VNU48_12010"
     CDS             6195..>6470
                     /locus_tag="VNU48_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HWI12046.1"
                     /translation="MNNWEMVLAILGLAVITVGTRGFFLYPERELPLPAWLRQGLRYA
                     PLAALAAVVAPEVVMTQGHLITTLKDARIYGTLAATAYFFWRRGILGT"
BASE COUNT          815 a         2303 c         2339 g         1013 t
ORIGIN      
        1 acgcaggccg cggacgccgc gaccgcgcag ctcgacacct gggtcgacac gctgctcgag
       61 aagcggcgct ggctgcgcgc gcgcgtggcg ggggcggcgc gatgaggaag gtcggcgtcg
      121 ttggctgggc gctcgcagcg ccggcgctgg tcgtgatctt cgtgttcttc gcactgccgg
      181 tcgcggcggg gctgctgctc agcctgaccg acttcgatct gtacgcgctg gccgacctgt
      241 cgacgctgcg cttcgtcggc ctgcagaact acgcgcggct gctgcagacg ccgctgttct
      301 ggcaggcgct gggcaacacc ttctacttcg tcctggtggg cgtgccggcg tcgatcggcc
      361 tgtcgctggg cgcggcgctg ctgctgaacg cgcgcgtcgc aaagtggcag ccgctgtttc
      421 gcaccgcgct gttcgcgccg gtcgtcacga ccgtggtcgc ggtcgcggtg atctggcgct
      481 acctgctgca cacgcgctac ggcctgatca accaggggct ggccacgctc ggcgtgtcgc
      541 ccgtcgactg gctcggcaac ccgcactggt cgatgccggc gatcatcctg ttcgcggcct
      601 ggaagaactt cggctacaac atgatcatcc tgctcgccgc gctgcaggcg gtgccgcgcg
      661 agctctacga atcggcgcgg gtcgacggcg ccggcacgct gcgccagttc gtcgacctga
      721 ccttcccgat gctgacgccc acgctcgtga tggtctcgat catgacgatc tcgggctact
      781 tccagctctt cgccgagccc tacgtgatga cgcagggcgg gccgctgcgc agcaccgtca
      841 gcgtgctgta cttcatgtac gaagaaggct ttcgctggtg gaacctgggc aacgcgtccg
      901 ccgtcgcgtt cctgctgttc gtcgtgatct tcgcggtgag ctgggtgctc ttgcgtttcg
      961 atcgcgatcc gtccggagcg accgcatgaa cgcgcgttgg gcggtgtggg tcgtcaacgg
     1021 gctgctcgcg gggctcgcgt tcgtcagcct gttcccgctg ctgtggatgc tgtcggtctc
     1081 gttcatgccg gccggcgcgt cgagcacctt gccgacgccg atcctcccgt cgcgcctcac
     1141 gctcgacaac taccgccagc tcttcggcag catcggcatg ggccgctaca tgctcaacag
     1201 cctggcgctc gcggtgctgt cgaccgtgat ctcggtcagc ttcaacgtga tggcgggcta
     1261 cgcgttcgcg aagctgcgct ttcgcgggcg cgatcgcgtc ttcaagacct tgctcggcgc
     1321 gctcgtgatc ccggggcagg tggcgatgct gccgctgttc ctgatgctca agccgctggg
     1381 cctgatcaac agctacggcg gcgcggtggt gccgatgatg gcgagcgtgt tcggcatttt
     1441 cctcgtgcgc cagttcgcgc gcggcatccc cgacgacctg ctcgaggccg cgcgcatcga
     1501 cggtgcgcac gagttccgca tcttcctgac catcgtcgtg ccgctgttga cgccggtgct
     1561 cgccacgctg gcgatctttt cgttcctggg cagctggaac gacttcatgt ggccgctgat
     1621 cgtgctgacc gacgattcgc tgcacacgct gccggtcgcg ctggccggcc tgtcgcgcga
     1681 gcacgtgcag gacaacgagc tgatgatggc cgggtcggtc gtcacggtgc tgccggtgct
     1741 ggtcttgttc ctcgcgctgc agcgccacta catggccggg ctgatggccg ggggcgtcaa
     1801 gggatgacgc cgcccgcgtg ggccgcgatc gcgctggtgg tgatcgctgc ggcggcgctg
     1861 ccggcacagg cgcaggagcg cgtgctcgac gacttcgccg acccgtcggc ctggaccgtg
     1921 agcgccaccg acgacgtcaa ggccagcctg cgcgctgccg acggcgcgcg cggccgcgcg
     1981 ctgtgcatcg actacgactt cggccgcgtc accggctacg tcacggcgca gcgtgcgttg
     2041 cccatcgagt tcccggcgcg ctacgagttc acgctgggcc tgcgcggcca gaccctgccg
     2101 aacgcgttcc agttcaagct ggtggacgcg agcggcgcga acgtctggtg gggccagcgc
     2161 cccgagttcc gtttccccgc gcaatggcag acgctgaagt tccgccagcg ccagatcgag
     2221 ttcgcgtggg gaccgatcgc cgatcgcacg ctgcgccgca gtgcctcggt cgagctcgtg
     2281 atcgcctccg gcagcggtgc cggcaagggc agtgcctgct tcgatcggct tacgctgcgc
     2341 gagctgccgc cggaggcgcc gccgctgccg ccgccgaaga tgacggcgga cgcgctcggt
     2401 gtcgacttcg gcgcgccgcg tgagttcggc gcgctcgcgc tgcgctggcg cgccggcgct
     2461 gccgcgcgct acgcgatcga gctgtcgaac gacggaacgc agtggcagcg cgtgcgcgag
     2521 gtcgagcgcg cgcgcggcga gctgcagcgg caatggctgc cggaatccga ggcgcgcttc
     2581 gtgcgcatcg tgccgcagcc ctcggccggg ccgccggtcg cgctcgccgc gatcgagccg
     2641 ctcgacgcac cgagcgccaa cctgttcttc gcgcagctcg cgcaggcggc gccgcgcggc
     2701 gcgtacccgc gcgcctaccg cggcgagcag tcgtactgga ccgtgctcgg tgtcgacggc
     2761 ggccgcaccg cgtcgctgct gagcgaggac ggcgtgctcg aagcgctgcc cggcatcggc
     2821 gcgctcgagc cgttcctcgt cgtcgacggt cacgtgctga actgggccga tgcgcgcatc
     2881 acgcacagcc tgcgcgacgg cgacctgccg atgccggctg tgcactggac gatcggcgat
     2941 ctgacgctcg acgtcgaggc cttcggcgcc ggcacgcccg aggcggcgca ggcgctggcg
     3001 cgctacacct tgcacaacgc gggcacacgc acgcaggcgg tcacgctcgc gctggcgtgg
     3061 cggccattcc aggcgaaccc gccgacgcag tttctcgcgc atccgggcgg cgcgagcgag
     3121 atcgcatcga tcgcatggga cggccgcgcg ctcgcggtcg atggcgtcgt gcgcttgcag
     3181 ccgctgaccg caccgagcgg cgtgcaggtc gagccgctcg cggctgggcc ggtcggcgac
     3241 tggctgcgcg acggcgcagc ctcggtggcg ccccagcgcg tagccgacgc ggccaggttc
     3301 gccagcggcg tgctgcgcta cgagtggcgt ctcgcgcccg gcgaacgacg cgaggtcgcg
     3361 atcgcgctgc cggtcagcgg cacgccgctg gcgccgacga tggatcagtt cgcgcacgag
     3421 cagcagcgtg tcgccgcgca ctggcgcgag cgcctggacc gggtgcgcat cagcggcccg
     3481 cacgaggtcg acgacatcgc gcgcacgttg cgcagcgcgc tcggccacat cctcgtcaat
     3541 cgcagcgggc cggcgttgca gccgggcgcg cgcgcgtatg cgcgctcgtg gatccgcgac
     3601 ggcgcactga cctcgtcggc gctgctgcgg ctgggccacg cggacgaggc gcgcgacttc
     3661 ctgcgctggt tcgcgccgtt ccagttcaag aacggcaagg tgccgtgctg tgcgaccgcg
     3721 cgcggtgccg acccggtgcc cgagaacgac agcgacggcg agttcgcgtt cgccgtcgcc
     3781 gaccactggc actacacgcg cgacgaagcg agcgcgcgcg cgctgtggcc gcacgtgcgc
     3841 gccgcggtcg cgcacatgga gggcctgcgc cagagcgagc gcaccgaggc gaaccgcacg
     3901 cccgagcgtg ccgcctactt cggcctgatg ccgccgtcga tcagccacga gggctactcc
     3961 gacaaggccg cgtattcgta ctgggacgac ttctgggccg cgatcggcta ccgcagcgcg
     4021 gtcgaactcg cgcaggcgct gggtctgccc gacgacgcga agcgcatcgc cgcgcagcgc
     4081 gacgagttcc tggtcgagct gcgcgcctcg atcgacgcga gcacgcggca tttcggtctc
     4141 gatgtgctgc ccggtgcggc cgaccgtggc gatgtcgacc cgacctcgag caccatcgca
     4201 ctcagccccg gcgggctgct cgccacgccg cccagcgcca ccgtgcgcaa caccttcgag
     4261 cgctactggc gcgacttcga ggcacggcgt gccgacgagg cggccggcgt gcgccacggc
     4321 gacggcggct acacgcctta cgagtggcgc aacgtcggcg cgctggtccg gctgggcgag
     4381 cgcgagcgcg cgctccaggc gctggcctac ttctaccgcg accgccgccc ggccggctgg
     4441 aaccagtggg ccgaggtcgt gctgcgcgat ccgcgcgagc cgcgttttct gggcgacatg
     4501 ccgcacggct gggtcgcgtc ggaccagatc cgttcggtgc tggacctgtt cgcctacgag
     4561 gacgagggca cccacgcgct ggtgctcgcc gccggcgtgc cgctgcgctg gttcgacgga
     4621 acgggcctgc gcgtgcaggg cctgcgcacg ccgtacggcg cgctcagctg gcaggcgcac
     4681 acgcacgctg cgagcggccg ccagcgcatc gacttcgaac tgcggccgct gcgcggctgg
     4741 cccgcgggcg gcgtcgagtt gcgcgggccg tggccggcgg atgcgcgggt ctgggtcgac
     4801 gggcgggcct gggccggccc ggccgacgcg atccggctga gcgtgacgcc ggcgcgggtg
     4861 cgcatcgaat ttgcgtcaac cgaatgaagg gcgccctgcc gcactcaagt cggtccggca
     4921 ccggccgata aacccggcag aactccctcc ggaacccacc gtcatgccgc gccgcgccct
     4981 cattgccctc gtcctcgcca gcagcctgct ggcgggttgc gacatgctgg tcggcgcgct
     5041 cggcatcgag tcgcccgaga aggtggcggc ggcgcgcgag gccgaaggca aggccatcgg
     5101 cggcgcctgc cgcaacgcgg cgcgcgcgat cgaggactgc ttcgcgttga accgcaaggc
     5161 cgacaaggcc gcggtgttcg cgggctggcg cgagatgaac gactacatgc gcgagaacaa
     5221 gatcgatccg gtcacgccgc aagtggtcac caaggtggcc ggcgcgaagg ccgcgaccga
     5281 ggccgaagaa gcggtcgagc cgcgcgcggc gaccaccaag ccggcggcga agaccgatgc
     5341 cgccaagccg cgtgcgcacg aggacgcgaa gccgcacgac tcctgactcc ggacctgacc
     5401 ggccgctgcg ctgcccccgc gcggcgccgg ccgcgcgcgc tcataggaca atcgccgccc
     5461 atgagtgacg ttctcgcgcg cccgatctgg cgccaccccg aattcgcgcg cggctcgcgc
     5521 gacatgatcg gcgtctcgct cggcatcgcg gcctgggggc tggtcaccgg cgtggcgatg
     5581 gccaagagcg ggttgccgct gccgatctcg atcctgatgt cgctgctcgt gttcgccggc
     5641 agtgcccagc tcgcggcgct gccgttgatc gtctcgggcg cgccgctctg gacggtctgg
     5701 gcgatcgcgc tgtgcgtgaa cctgcgcttc gtgatcttca gcgcgcagtg gcgaccgtac
     5761 ttcgcgcacc tgccgcgctg gcagcgcatg ctgcgcggct acttcaccgc cgacctgaac
     5821 tatgtggtgt tcatggcgcg cttcccgcag ccccggcccg cgcccgagca ggagccgtac
     5881 ttctggggtg gcgtcgtgac gaactggccg tcgtggcagc tgccgacctt ggccggcctg
     5941 ctgctcggcg acgcgatccc gaccgaatgg ggcttcggct tcgccggcat cctggcgctg
     6001 ctggggctga tgtattcgct gctgggcgat cgggccagct gggtcgcggc cggcgtggcc
     6061 ggcgccgccg cggtcgccac cgcggcgctg ccgctgaagc tcaacgtggt cgtcgcgatc
     6121 gccgcggcgg tggcgatcgg cctgctgatg gatcacgtga cgccggccga ggcgaagccg
     6181 gtggaggaca acacgtgaac aactgggaga tggtgctcgc gattctcggc ctggcggtga
     6241 tcacggtcgg cacgcgcggc ttcttcttgt accccgagcg cgagctgccg ttgccggcct
     6301 ggctgcgcca gggcctgcgc tacgcgccgc tggccgcgct cgccgcggtc gtcgcgcccg
     6361 aggtcgtgat gacccagggc cacctgatca cgaccttgaa ggacgcgcgg atctacggca
     6421 cgctcgccgc gacggcctat ttcttctggc gccgtggcat cctcggcacc
//
LOCUS       DATMJB010000561         2285 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_393517, whole genome shotgun sequence.
ACCESSION   DATMJB010000561 DATMJB010000000
VERSION     DATMJB010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393517"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /gene="phaZ"
                     /locus_tag="VNU48_12015"
     CDS             <1..892
                     /gene="phaZ"
                     /locus_tag="VNU48_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HWI12047.1"
                     /translation="FITDWTDARMVPASEGPFHLNDYVTCVQEFIRHIGPAVNVISVC
                     QPTVPVLAAIALMASRGEPTPRTMTMMGGPIDARKSPTAVNNLATTKTHAWFENNVIQ
                     RVPTNFPGSGRRVYPGFLQHTGFVAMNPDRHLSSHYDYFLDLVRGDDSSAEFHRDFYD
                     EYNAVLDMPAEYYLDTIKVVFQDFALVNGNWRVKGELVRPQDIKTSALLTIEGELDDI
                     SGAGQTRAAHELCTGIPKNRQFHYDAIGAGHYGIFSGRRWREKIYPRVREFIASHQPT
                     AAAKPAARAEVAKKAARKAR"
     gene            901..1470
                     /locus_tag="VNU48_12020"
     CDS             901..1470
                     /locus_tag="VNU48_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HWI12048.1"
                     /translation="MSDAHALVQALTDALPQTQCTRCGFPDCRSYAQAIAHDGAPINQ
                     CPPGGAQGIARLAALTGRPQQPLNPANGCEAPRVLAVIDEDWCIGCTLCLDACPVDCI
                     VGGPKAMHTVIAAQCTGCELCLPACPVDCIALRPVTGALTGWDAWSDAQAREAGERFA
                     WHGQRLERDARERADRLAANAPPQRVASP"
     gene            1467..2150
                     /gene="nth"
                     /locus_tag="VNU48_12025"
     CDS             1467..2150
                     /gene="nth"
                     /locus_tag="VNU48_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HWI12049.1"
                     /translation="MKSADIEPFFATLKAANPQPETELEYTSVFELLAAVLLSAQATD
                     VGVNKATRRLFAVANTPAKVLALGLDGLTEYIRTIGLYRTKAKNLLATCRILIEQHGG
                     RVPAQRAALEALPGVGRKTANVVLNVAFGEATMAVDTHIFRVANRTGLAPGRNPLEVE
                     LKLLQRVPPPYRVDAHHWLILHGRYVCQARRPLCGQCSVTRWCDAFPAITRGAHPMIE
                     RASQRYHSR"
BASE COUNT          386 a          834 c          749 g          316 t
ORIGIN      
        1 cttcatcacc gactggaccg acgcacgcat ggtgccggcc agcgaggggc cgttccacct
       61 caacgactat gtgacctgtg tgcaggagtt catccgccac atcggcccgg cggtcaacgt
      121 gatctcggtg tgccagccga ccgtgccggt gctcgcggcg atcgcgctga tggccagtcg
      181 cggcgagccc acgccgcgca cgatgacgat gatgggcggc ccgatcgacg cccgcaagag
      241 cccgaccgcc gtcaacaacc tggcgacgac caagacccac gcatggttcg agaacaacgt
      301 gatccagcgt gtgccgacga atttccccgg cagcggccgc cgcgtctacc cgggctttct
      361 gcagcacacc ggtttcgtcg cgatgaaccc ggaccggcat ctgagttcgc actacgacta
      421 cttcctggac ctggtgcgcg gcgacgacag cagtgccgag ttccaccgcg acttctacga
      481 cgagtacaac gccgtgctgg acatgccggc cgagtactac ctcgacacca tcaaggtcgt
      541 gttccaggat ttcgcgctcg tcaacggcaa ctggcgggtc aagggtgaac tggtgcggcc
      601 gcaggacatc aagacgagtg cgctgctcac catcgaaggc gaactcgacg acatctccgg
      661 cgccggccag acgcgcgccg cgcacgagct gtgcaccggc atcccgaaga accgccagtt
      721 ccactacgac gcgatcggtg ccggccacta cggcatcttc tcgggtcggc gctggcgcga
      781 gaagatctac ccgcgcgtgc gcgagttcat cgccagccac cagccgacgg cagcggcgaa
      841 gccggccgcg cgcgccgagg tagccaagaa ggcagcgcga aaggcccggt gaaccgtgcg
      901 gtgagcgacg cccacgcgct cgtgcaggcg ctcaccgacg cgctgccgca gacccagtgc
      961 acgcgctgcg gctttcccga ctgccgcagc tacgcgcagg cgatcgccca tgatggcgcg
     1021 ccgatcaacc agtgcccgcc cggcggtgcc caaggcatcg ccagactcgc cgccctcacc
     1081 ggccggccgc agcagccgct aaatcccgcc aacgggtgcg aggcaccgcg cgtgcttgcg
     1141 gtcatcgacg aggactggtg catcggctgc accttgtgcc tggacgcctg cccggtcgac
     1201 tgcatcgtcg gcggcccgaa agccatgcac acggtgatcg ccgcgcaatg caccggctgc
     1261 gagctgtgcc tgccggcctg cccggtcgac tgcatcgcgc tgcggccggt gacgggcgca
     1321 ctcaccggct gggatgcgtg gagcgacgcg caggcgcgtg aggccggcga gcgcttcgcc
     1381 tggcacgggc agcggctgga acgcgacgcg cgcgagcgcg ccgaccgctt ggccgccaac
     1441 gccccgccac agcgcgtggc cagcccatga agagcgccga catcgagccc ttcttcgcga
     1501 ccctgaaggc cgcgaacccg caacccgaaa ccgagctcga gtacaccagc gtgttcgaac
     1561 tgctggccgc ggtgctgctg tcggcgcagg ccaccgacgt cggcgtcaac aaggccacgc
     1621 ggcgcctgtt cgcggtcgcg aacacgccgg ccaaggtgct cgcgctcggc ctggacggac
     1681 tgaccgaata catccgcacg atcggcctgt accgcacgaa ggcgaagaac ctgctcgcga
     1741 cctgccgcat cctgatcgag cagcacggtg gccgcgtgcc ggcgcagcgt gcagcgctcg
     1801 aggcgctgcc cggcgtgggc cgcaagaccg ccaacgtggt gctgaacgtc gccttcggcg
     1861 aagccacgat ggccgtggac acgcacatct tccgtgtcgc caaccgcacc ggcctcgcgc
     1921 cgggccgcaa cccgctggag gtcgagctga agctgctgca acgcgtgccg ccgccctacc
     1981 gcgtcgacgc gcaccactgg ctgatcctgc acgggcgcta tgtgtgccag gcacgacggc
     2041 cgctgtgcgg gcagtgctcg gtcacgcgct ggtgcgacgc gttcccggcg atcacgcggg
     2101 gcgcgcatcc catgatcgag cgtgcatcgc agcgttatca cagccgataa tcgcgcacat
     2161 gacagagcct tccgacctgc aggccatctc cggccgcctc gagcgcggcg agatcgacgg
     2221 tgtgcgctac ttcgaagagc tcacacgctt cgtcgccgcg cagatcggct gttcgcgtgc
     2281 cggcc
//
LOCUS       DATMJB010000562         7708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_149571, whole genome shotgun sequence.
ACCESSION   DATMJB010000562 DATMJB010000000
VERSION     DATMJB010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149571"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VNU48_12030"
     CDS             complement(<1..225)
                     /locus_tag="VNU48_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease domain-containing protein 2"
                     /protein_id="HWI12050.1"
                     /translation="MNDAPFTPGKEEIAALEPFERLPLGRIDLIATAAQAERAIAELA
                     GAAVLGFDTESKPTFAKGEVSDGPHIVQLAT"
     gene            complement(222..1265)
                     /locus_tag="VNU48_12035"
     CDS             complement(222..1265)
                     /locus_tag="VNU48_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWI12051.1"
                     /translation="MRIESIRQRLREHGAQPAHEHRVLRLWACALAQDSGKRKPEDFL
                     PRSVRAMLPALVDELAGLARLRSEHPAADGSSRLLVELADGQAVESVLLPRGGLCVST
                     QLGCAVGCVFCMTGRDGLLRQLGSAEIVAQLVLARRRRAVRKVVFMGMGEPAHNLDNV
                     IEAIELLGTAGGIGHKNLVFSTVGDLRVFERLPRAAVKPALALSLHSTKPDLRARLLP
                     RAPRLAPAELVAHGEHYARATGYPIQYQWTLLAGINDGDDELDGIVELLRGKYALLNM
                     IPYNRVEGLAFERPPRERAEAIARTLLRRGVLTKLRWSAGQDVEAGCGQLRARAAPTQ
                     VVTLRRSAAPELA"
     gene            1398..2099
                     /locus_tag="VNU48_12040"
     CDS             1398..2099
                     /locus_tag="VNU48_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12052.1"
                     /translation="MFGRSRPVFFEPHGRRRRAGPPRWLVLLLVGIALGAAGVVVVQE
                     RYLPPRLSAEAGAQLRDAFEHASTERASLRRELDQTGQRLSAALTERQRLAGELATSR
                     AATAGLQDDLAAAVDALPADPRGGAVQVRAARFTTARDGSLAYEVVLTREHAAGKALT
                     GQLQLVVAGEAARGPDPRVTLTPIAFTMGNRVVLRGSAPLPAGFRARQSTIQVLGGGK
                     ALGMRVLRVERGAAD"
     gene            2453..4480
                     /locus_tag="VNU48_12045"
     CDS             2453..4480
                     /locus_tag="VNU48_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008167312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWI12053.1"
                     /translation="MVVTATRADARAFDVPASIDRIDAATVRDGHALVNISESLGGVP
                     GLIARDRQNYAQDVQISVRGFGARSTFGIRGVRLYVDGIPATLPDGQGQITNVELGSV
                     GRIEVLRGPFSALYGNSSGGVIQVFTEEGQGPPTLSFGASAGSDGLLRYGAQASGSTG
                     AMSYSLSASRFRTDGYRDHSAVERTLGNAKLTLRPDGDSRVTLIANSVGLPQAQDPMG
                     LSRALFDANPRGVDPSALDYNTRKSVNQTQAGLVYERRLDAANALQLTTYYGHRGTEQ
                     FQSIPKSTQGGPLNPGGVIQLARNYGGSDLRWTFKSNLLDAPFTLVGGVAYDSLTEHR
                     QGFQNFIGTTLGVQGALRRDEINDVSNFDQYLQAAWQLTPQWSINAGVRHSQVRFTST
                     DGYIVGPNPDDSGAARYGATLPVLGVMYAVTDDLHVYATAGRGFETPTLNELAYRPGG
                     ASGLNFGLQAATSTSVEAGVKARVASLGELTAAVFQTGTDREIVTQTNSGGRATFQNA
                     GRTRRTGVELGWSATYAESLRAQLAATLLNATYRDGFLTCTSTPCATANVPIPAGNRI
                     PGIARGTLFAALNWAPPIGLRGGVEARYVTGVPVNDLNSDAAPRYAVAAANVGYVFRA
                     GAWELGGFVRGDNLFDRRYAGSVIVNEGNGRFFEPAPGRTWLASASATLRF"
     gene            4489..5064
                     /locus_tag="VNU48_12050"
     CDS             4489..5064
                     /locus_tag="VNU48_12050"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HWI12054.1"
                     /translation="MTTTDTAHYGRFASALHWLIGIAMLVEIVFGFLLDDIAPRGTPA
                     RAAVINLHKSTGVLLGLLVVLRLVWRLRHAPPPWPTSMPAWQRSAAHLLHIAMYVCML
                     VLPLSGYVASNLSKNGLRVFGYWLRPWGPDLPAAYAALTRVHDLSAWLFAVLVAGHIA
                     VALKHALVDRDDIFARVWPWAGARLTSREAP"
     gene            5061..6008
                     /locus_tag="VNU48_12055"
     CDS             5061..6008
                     /locus_tag="VNU48_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWI12055.1"
                     /translation="MTRPLRCLALTLTLLAPFASFAAPFAYVPNEGSGTVSVIDTQTD
                     KVVYELPAGKKPRGTVVGLDGTRAYVSDQPNNRLVMLDLTKRAQAGSIDIGESPEGLG
                     ISADGRWIAAAIEESNDIVFIDTRTNTKTFTVAVRGKNPEHAIFSPDGRLVFVSAEEG
                     EAIDVIDVAKRTEVAQVPVGARPRGIGFSPDSTRAYVGAENSSEVYVIDAVAFTVLAK
                     VKAGARSNGIAVHPDGKRVFVSNGGDASVSVLDTATNAITATIGVGQRPWNMALTPDG
                     KKLYVACGRSGVVSVIDTERNLKIADVPVGKLPWGVAIR"
     gene            6066..7076
                     /locus_tag="VNU48_12060"
     CDS             6066..7076
                     /locus_tag="VNU48_12060"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HWI12056.1"
                     /translation="MHVFRGFHHPGIAPACALTIGNFDGVHRGHQAMLALLNSEARHR
                     GLPSCVMTFEPHPRDYFAALAGRPELAPARIATLRDKLSELERCGIEQVVVLRFDRRF
                     AAQAPQVFIDDVLVRGLGTKYVLVGDDFRFGAKRAGDYAMLDAAGPQAGFDVARMNSY
                     EVRGLRVSSSAVREALAAGDMARAAALLGRPYSISGHVVHGRKLGRGLGFPTLNLRFS
                     HPKPAALGIYVVRVHGLTERALDGVASLGKRPTVDDSGRVLLETYCLDWPAALGAEGG
                     YGKLVRVELLHKLRDEARYDGLPALTAAIEKDVVDASAWLASHPSAHAAEHRQTSRDR
                     II"
     gene            7183..>7708
                     /locus_tag="VNU48_12065"
     CDS             7183..>7708
                     /locus_tag="VNU48_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWI12057.1"
                     /translation="MSTTKTDYRSTLNLPDTPFPMRGDLPKREPGWVKEWTDQGIYQR
                     LRDARVGKPRFVLHDGPPYANGTLHVGHAANKILKDMIVKARQLKGLDARYTPGWDCH
                     GLPIENQIEKTHGRKLPRAEVQAKSRAYATEQIALQMADFKRLGVLGDWAHRYATMDP
                     KNEAGEIRAFKRVIE"
BASE COUNT         1143 a         2833 c         2670 g         1062 t
ORIGIN      
        1 cgtcgcgagc tgcacgatgt gcgggccgtc ggagacctcg cccttcgcga aggtcggctt
       61 cgattcggtg tcgaagccga gcaccgcggc gccggccagt tcggcgatcg cgcgctcggc
      121 ctgcgcggcc gtcgcgatca ggtcgatccg gcccagcggc aggcgctcga acggctcgag
      181 cgcggcgatc tcttccttgc ccggcgtgaa cggggcgtcg ttcatgcgag ctccggtgcg
      241 gcgctgcgtc gcagcgtgac gacctgcgtc ggcgcagcac gtgcacgcag ctgcccgcag
      301 ccggcctcga cgtcctgccc ggccgaccag cgcagcttgg tcagcacgcc gcgccgcagc
      361 agcgtgcgcg cgatcgcctc ggcgcgctcg cgcggcgggc gttcgaacgc cagcccctcg
      421 acgcggttgt acgggatcat gttcagcagc gcgtacttgc cgcgcagcag ctcgacgatg
      481 ccgtcgagct cgtcgtcgcc gtcgttgatg ccggccagca gcgtccactg gtactggatc
      541 gggtagccgg tggcgcgcgc atagtgctcg ccgtgcgcga ccagctcggc cggcgcgagg
      601 cgcggcgcgc gcggcagcag gcgcgcgcgc aggtcgggct tcgtcgaatg cagcgacagc
      661 gcgagcgccg gcttcaccgc ggcgcgcggc agccgctcga acacgcgcag gtcgcccacc
      721 gtcgagaaca ccaggttctt gtggccgatg ccaccggcgg tgccgagcag ctcgatcgcc
      781 tcgatcacgt tgtccaggtt gtgcgccggc tcgcccatgc ccatgaacac gaccttgcgc
      841 accgcgcgcc ggcggcgcgc gaggacgagc tgcgcgacga tctcggcact gcccagctgg
      901 cgcagcaggc cgtcgcgccc ggtcatgcag aacacgcagc cgaccgcgca gcccagctgc
      961 gtcgacacgc acagcccgcc gcgcggcagc agcacgctct cgaccgcctg gccgtcggcg
     1021 agctcgacca gcaggcgcga cgaaccgtcg gcggccgggt gctcggagcg cagccgcgcc
     1081 aggccggcca gttcatcgac cagcgccggc agcatcgcgc gcacgctgcg cggcaggaag
     1141 tcctcgggct tgcgcttgcc gctgtcctgc gcgagcgcac acgcccacag ccgcagcacg
     1201 cggtgctcgt gagcaggttg ggcaccgtgt tcgcgaaggc gttgccggat ggattcgatg
     1261 cgcatgagcg cgcgagttta ggcgctcctc gccacgcgga tgtctgccgc ctggcggcgc
     1321 cccgaaacat ggcgtttcag tttgtgtatc gcagcgcggt acgctcgccc ctcgtttcac
     1381 gacacctcgc cccgcgcatg ttcggacgct cccgacccgt cttcttcgag ccccatggtc
     1441 gccgccggcg cgccggcccg ccgcgctggc tggtgctgct gctggtgggc atcgcgctcg
     1501 gcgcggccgg ggtcgtcgtc gtgcaggagc gctacctgcc gccgcgcctg tcggccgagg
     1561 ccggtgcgca gctgcgcgat gcgttcgagc acgccagcac cgaacgcgcg tcgctgcgcc
     1621 gcgagctcga ccagaccggc cagcggctca gtgcggcgct caccgaacgt cagcgactgg
     1681 cgggcgaact cgcgacgagc cgtgccgcga ccgcaggcct tcaggacgac ctggccgccg
     1741 ccgtcgatgc gctgccggcc gacccgcgcg gcggtgcggt ccaggtgcgc gccgcacgct
     1801 tcacgacggc gcgcgatggc agcctggcct acgaagtggt gttgacgcgc gaacacgccg
     1861 ccggcaaggc attgacgggc cagctgcagc tggtggtcgc cggcgaggcc gcacgcggcc
     1921 ccgacccgcg cgtcacgctg acgccgatcg cgttcacgat ggggaaccgc gtcgtgctgc
     1981 gcggcagcgc gccgctgccc gcgggattcc gggcacgcca gtcgacgatc caggtgctcg
     2041 gcggcggcaa ggcactcggg atgcgggtgc tgcgcgtcga gcgcggcgcg gcggactgaa
     2101 accgcaggct gtgcggacgg cgtgccgacc cggcacacgg cgcggaagac gccgctgctg
     2161 tcccaaaaaa tgggacaggg caaacctcgg ggacagcgtt tgtggaagcc aggccacatt
     2221 taccatcgct tggacgcgac ggaccacacc ccgcgcggga tctgacgaac gacctgatcg
     2281 atacttgacg gcccccccgc gaagatgggc cccctgaggt tccggagaca agatgcaagc
     2341 cacgttcgat ctgtgccgct ccgtgcacgt gctttgctgg ttcgcagcgg ccccggcctg
     2401 cgcgctcgcc caggccgggg cgcccgcgcc ggcaaccccg cagctcccgg caatggtcgt
     2461 cacggccacg cgcgccgatg cgcgtgcgtt cgacgtgccg gcgtcgatcg accggatcga
     2521 cgccgccacc gtgcgcgacg gccacgcgct ggtcaacatc tccgagagcc tgggaggcgt
     2581 gcccgggctg atcgcgcgcg accgccagaa ctacgcgcag gacgtgcaga tctcggtgcg
     2641 cggcttcggc gcgcgctcga ccttcggcat ccgcggcgtt cggctgtatg tcgacggcat
     2701 cccggcgaca ctgcccgatg gccagggcca gatcaccaac gtcgagctcg gctcggtcgg
     2761 gcgcatcgag gtgctgcgtg ggccgttctc ggcgctgtat ggcaactcgt cgggcggcgt
     2821 gatccaggtc ttcaccgaag agggccaggg gccgccgacg ctcagcttcg gcgcgtcggc
     2881 cggcagcgac ggcctgctgc gctacggcgc gcaggcgagc ggctcgaccg gcgcgatgag
     2941 ctactcgctg agcgcgagcc gcttccgcac cgacggctac cgcgatcaca gcgcggtcga
     3001 gcgcacgctg ggcaatgcca agctcacctt gcgcccggac ggcgactcca gggtgacgct
     3061 gatcgccaac agcgtcgggc tgccgcaggc gcaggacccg atgggcctgt cgcgcgcgct
     3121 gttcgacgcc aacccgcgcg gcgtcgaccc atcggcgctc gactacaaca cccgcaagtc
     3181 ggtgaaccag acccaggccg gcctggtcta cgagcgccgc ctcgatgcgg ccaatgcgct
     3241 gcagctgacg acctactacg ggcatcgcgg caccgagcag ttccagtcga tcccgaagag
     3301 tacccaaggc ggcccgctga acccgggtgg cgtgatccag ctcgcgcgca actacggcgg
     3361 gagcgatctg cgctggacct tcaagtcgaa cctgctcgac gcgccgttca cgctggtcgg
     3421 cggcgtcgcc tacgacagcc tgacggaaca ccgccagggc ttccagaact tcatcggcac
     3481 cacgctcggc gtgcagggtg cgctgcgccg cgacgagatc aacgacgtgt cgaacttcga
     3541 ccagtacctg caggcggcct ggcagctcac gccgcagtgg tcgatcaacg cgggggtgcg
     3601 ccacagccag gtgcgtttca cgtcgaccga cggctacatc gtcggcccca accccgacga
     3661 cagcggcgcg gcgcgctacg gcgcgaccct gccggtgctc ggcgtgatgt acgcggtgac
     3721 cgacgacctg cacgtctatg ccaccgcggg gcgcggcttc gagacgccga cgctgaacga
     3781 gctcgcctac cgccccggcg gcgcgagcgg cctgaacttc ggcctgcagg cggccaccag
     3841 caccagcgtc gaggccggcg tgaaggcgcg cgtcgcatct ctcggcgagc tgaccgcggc
     3901 ggtgttccag accggcaccg acagggagat cgtgacgcag accaacagcg gcggccgcgc
     3961 caccttccag aacgccggcc ggacgcgccg caccggcgtc gagctcggct ggtcggcgac
     4021 ctatgccgag tcgctgcgcg cgcagctcgc cgcgaccctg ctgaacgcga cctatcgcga
     4081 cggcttcctg acctgcacgt ccacgccctg cgcgacggcg aacgtcccga tcccggccgg
     4141 caaccgcatc cccggcatcg cgcgcggcac gctgttcgcg gcgctgaact gggcgccgcc
     4201 catcggtctg cgcggcggtg tcgaggcgcg ctacgtgacc ggcgtgccgg tcaacgacct
     4261 gaacagcgac gcagcgccgc gctacgccgt cgcggcggcc aacgtcggct acgtgttcag
     4321 ggccggcgcc tgggagctgg gcggcttcgt gcgcggcgac aacctgttcg accgccgcta
     4381 cgccggctcg gtgatcgtca acgagggcaa cggccgcttc ttcgaaccgg cgcccggccg
     4441 gacctggctc gccagcgcgt cggcgacgct gcgcttctga acccgaccat gacgacgacc
     4501 gacacggccc actatggccg cttcgccagc gcgctgcact ggctgatcgg gattgcgatg
     4561 ctggtggaga tcgtcttcgg cttcctgctc gacgacatcg cgccgcgcgg cacgccggcg
     4621 cgcgccgcgg tcatcaacct gcacaagtcg acgggcgtgc tgctcgggct gctggtcgtg
     4681 ctgcggctcg tatggcgcct gcggcacgcg ccgccgccgt ggcccacatc catgccggcc
     4741 tggcagcgca gcgctgcaca cctgttgcac atcgcgatgt acgtctgcat gctggtgctg
     4801 ccgctgtcgg gctacgtcgc atcgaacctc agcaagaacg ggctcagggt gttcggctac
     4861 tggctgcggc cctggggccc cgatctgccg gcggcctacg ccgcactcac gcgggtccac
     4921 gacctgagcg cgtggctgtt cgcggtgctg gtggccggcc acatcgcggt cgcgctgaag
     4981 cacgcgctgg tcgatcgcga cgacatcttc gcgcgcgtct ggccctgggc cggtgcacgc
     5041 ctcacttccc gggaagctcc atgacgcgtc cgctgcgctg ccttgccctg accctgaccc
     5101 tgctcgcacc gttcgcgagc ttcgccgcgc ccttcgccta cgtgcccaac gagggctcgg
     5161 gcaccgtgag cgtgatcgac acgcagaccg acaaggtcgt ctacgagctg cccgccggca
     5221 agaagccgcg cggcaccgtg gtcggcctcg acggcacgcg cgcctacgtg agcgaccagc
     5281 cgaacaaccg gctggtgatg ctcgacctca ccaagcgcgc gcaggccggc agcatcgaca
     5341 tcggcgaatc gcccgagggc ctgggcatct cggccgacgg ccgctggatc gccgcggcga
     5401 tcgaggagtc gaacgacatc gtcttcatcg acacccgcac gaacaccaag acgttcacgg
     5461 tggccgtgcg cggcaagaac cccgagcacg cgatcttcag tccggacggt cgcctcgtgt
     5521 tcgtcagcgc cgaggaaggc gaggcgatcg acgtgatcga cgtcgccaag cgcaccgagg
     5581 tcgcgcaggt gccggtgggc gcgcggccgc gcggcatcgg cttctcgccc gacagcacgc
     5641 gcgcctacgt gggcgcggaa aattcgagcg aggtctacgt gatcgacgcc gtcgcgttca
     5701 cggtgctggc caaggtcaag gccggcgcgc gcagcaacgg catcgcggtg caccccgacg
     5761 gcaagcgcgt gttcgtctcg aacggcggcg atgccagcgt ctcggtgctc gacaccgcca
     5821 ccaacgcgat caccgccacg atcggcgtcg gccagcggcc gtggaacatg gcactcacgc
     5881 ccgacggcaa gaagctgtac gtggcctgcg gccgctccgg cgtggtctcg gtgatcgaca
     5941 ccgagcgcaa cctcaagatc gccgacgtcc ccgtcggcaa gctgccctgg ggcgtcgcga
     6001 tccgctgagc aggtgtgaac gaacccccac cgcagcccgc gcagccccgg ccgataatgc
     6061 cgcgcatgca cgtgttccgc ggctttcatc accctggcat cgcgcccgcc tgcgcgctca
     6121 cgatcggcaa tttcgacggc gtgcaccgcg gccaccaggc gatgctggcg ctgctgaact
     6181 cggaggcccg gcaccgcggc ctgccgtcgt gcgtgatgac cttcgagccg catccgcgcg
     6241 actacttcgc tgccctcgcc ggccggcccg agctcgcgcc ggcacgcatc gcgaccctgc
     6301 gcgacaagct cagcgagctc gagcgctgcg gcatcgagca ggtggtcgtg ctgcgcttcg
     6361 accggcgctt cgcggcgcag gcgccgcagg tcttcatcga cgacgtgctg gtgcgcggcc
     6421 tgggcacgaa gtacgtgctg gtcggcgacg acttccgctt cggcgcgaag cgcgccggcg
     6481 attacgcgat gctcgacgcc gccggcccgc aggccggttt cgatgtcgcg cgcatgaaca
     6541 gctacgaagt gcgcggtctg cgcgtgtcga gttcggcggt gcgcgaggca ctcgcggccg
     6601 gcgacatggc gcgcgccgcg gcgctgctgg ggcggccgta cagcatcagc gggcatgtcg
     6661 tgcacggcag gaagctcggc cgcggcctgg gcttccccac gctgaacctg cgcttcagcc
     6721 acccgaagcc ggccgcgctg ggcatctacg tggtgcgcgt gcacggcctc accgagcgag
     6781 cgctcgacgg tgtcgcgagc ctgggcaaac gcccgaccgt ggacgacagc ggccgtgtgc
     6841 tgctcgagac ctactgcctc gattggcccg ccgcgctcgg cgccgagggg ggctacggta
     6901 aactcgtgcg cgtggaactg ctgcacaaac ttcgcgacga agcccgctac gacgggctgc
     6961 ccgccctgac cgccgcgatc gagaaagacg tggtcgatgc gagcgcctgg ttggcctcac
     7021 acccgagcgc ccacgccgcc gagcaccgcc agaccagccg cgaccgaatt atttgacggg
     7081 caccccgccc gccgatcttt cgaatcgcgc ccgccgcagt cgcgggcttc aactctgatt
     7141 cgctgcccgc cgcggggacc gagccgcacc atgagcgcca ccatgtcgac caccaagact
     7201 gactaccgat ccacgctgaa cctgcccgac acgcccttcc cgatgcgcgg cgacctgccc
     7261 aagcgcgagc cgggctgggt caaggaatgg acggatcagg gcatctatca gcgcctgcgt
     7321 gatgcgcgcg tcggcaagcc gcgcttcgtg ctgcacgacg gcccgccgta cgcgaacggc
     7381 acgctgcacg tcggccatgc ggccaacaag atcctgaagg acatgatcgt caaggcgcgc
     7441 cagctgaagg gcctggacgc gcgctacaca ccgggctggg actgccacgg cctgccgatc
     7501 gagaaccaga tcgagaagac ccacggccgc aagctgccgc gcgccgaggt gcaggccaag
     7561 agccgcgcct acgcgaccga gcagatcgcg ctgcagatgg cggacttcaa gcgcctgggc
     7621 gtgcttggcg actgggcgca ccgctacgcg acgatggacc cgaagaacga ggccggcgag
     7681 atccgcgcgt tcaagcgcgt gatcgagc
//
LOCUS       DATMJB010000563         2156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_792834, whole genome shotgun sequence.
ACCESSION   DATMJB010000563 DATMJB010000000
VERSION     DATMJB010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_792834"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /gene="prpE"
                     /locus_tag="VNU48_12070"
     CDS             complement(<1..1419)
                     /gene="prpE"
                     /locus_tag="VNU48_12070"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HWI12058.1"
                     /translation="MTRYADFHRRSLADRDGFWAEQARLIDWQRPFDQVCDYGRPPFA
                     RWFVGGRTNLCHNAVDRHLADRSDQNALIWVSTETDEETVYSYRELHAEVQRMAACLL
                     ALGVKQGDRVLIYMPMIAPAAFAMLACARIGAIHSVVFGGFASGSLADRIDDAQPTVI
                     VSADAGSRSGKVIAYKPLLDEAIRLAAHKPEKVLLVDRGLAAMERVLDRDVDYAALRA
                     QHLDAVVPCVWLDSTAPSYTLYTSGTTGKPKGVQRDTGGYAVALAASMKHIFCGNAGE
                     TYFSTSDIGWVVGHSYIVYGPLIAGMATIMYEGLPTWPDAGIWWKLVEKYKVTAMFSA
                     PTAVRVLKKQDPKFLSQYDLSSLRALFLAGEPLDEPTAQWISESLGRPIIDNYWQTES
                     GWPILTIANGVEAAPSKFGSPGVAMYGYDVKLIDETNGAEITEPNRKGVLAIAGPLPP
                     GCMQTVWRDDERFVNTYWKDFAG"
     gene            complement(1446..>2156)
                     /locus_tag="VNU48_12075"
     CDS             complement(1446..>2156)
                     /locus_tag="VNU48_12075"
                     /EC_number="1.3.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acryloyl-CoA reductase"
                     /protein_id="HWI12059.1"
                     /translation="GVGETRWGCLAERARLMGDWLVPLPAAFSTLQAMAIGTAGYTAM
                     LCVLALERHGVKAGDGEVLVTGATGGVGSVAVALLGRLGHRVIAATGKPGEAGYLHAL
                     GAAGVIDRAELSAPGKPLQKERWAAVVDAVGSHTLVNALAQTRYGGVVAACGLAQGFD
                     LPATVMPFILRGVTLAGVDSVMAPLAARRLAWERLAVDLDAAKLAAITELVPLAAAIE
                     KARDLMQGKVRGRIVVKI"
BASE COUNT          321 a          772 c          727 g          336 t
ORIGIN      
        1 cccggcgaag tccttccagt acgtgttgac gaagcgctca tcgtcgcgcc acaccgtctg
       61 catgcagccc ggcggcaacg gcccggcgat cgcgagcacg cccttgcggt tcggctcggt
      121 gatctcggcg ccgttggtct cgtcgatcag cttcacgtcg tagccataca tcgccacgcc
      181 cgggctgccg aacttgctgg gcgccgcctc gacgccgttg gcgatcgtca ggatcggcca
      241 gccggattcg gtctgccagt agttgtcgat gatcggccgg cccaggctct cggagatcca
      301 ttgcgccgtt ggctcgtcca gcggttcgcc ggccaggaac agcgcgcgca gcgagctcag
      361 gtcgtactgg ctcaggaatt tcgggtcctg cttcttcagc acgcgcaccg cggtcggcgc
      421 actgaacatc gccgtgacct tgtacttctc gaccagcttc caccagatgc cggcgtcggg
      481 ccaggtcggc aggccttcgt acatgatcgt cgccatgccg gcgatcagcg gcccgtagac
      541 gatgtagctg tggccgacga cccaaccgat gtcgctggtc gagaagtagg tttcccccgc
      601 gttgccacag aagatgtgct tcatgctcgc ggccagcgcc accgcgtagc cgccggtgtc
      661 gcgctgcacg cccttgggct tgccggtggt gccgctggtg tagagcgtgt agctgggcgc
      721 ggtcgagtcg agccagacgc agggcacgac ggcgtccagg tgctgcgcgc gcagcgccgc
      781 gtagtcgacg tcgcggtcga ggactcgctc catcgccgcc agcccccggt cgacgagcag
      841 cactttctcg ggcttgtgcg cggccaggcg gatcgcctcg tccagcaacg gcttgtaggc
      901 gatgaccttg cccgagcggc tgccggcgtc ggcgctgacg atcacggtcg gttgcgcgtc
      961 gtcgatgcgg tccgccaggc tgccgctggc gaagccgccg aacacgaccg aatggatcgc
     1021 cccgatgcgc gcacaggcca gcatcgcgaa cgcggccggc gcgatcattg gcatgtagat
     1081 cagcacccgg tcgccctgct tcacgccgag ggcgagcaga caggcggcca tgcgctggac
     1141 ttcggcgtgc aactcgcggt agctgtagac cgtttcctcg tcggtctcgg tcgacaccca
     1201 gatcagcgcg ttctggtcgg accgatcggc gaggtgccgg tcgaccgcgt tgtggcacag
     1261 gttggtccga ccaccgacga accagcgcgc gaacggcggc ctgccgtagt cgcagacctg
     1321 gtcgaacggc cgctgccagt caatcagacg ggcctgttcg gcccagaagc catcgcggtc
     1381 ggcgagcgag cggcgatgaa agtcggcata gcgcgtcatg gttcgggggc gtctgtggtc
     1441 ggaggtcaga tcttgacgac gatgcgcccg cgcaccttgc cctgcatcaa gtcccgcgcc
     1501 ttctcgatcg ccgcggccag cggcacgagc tcggtgatcg cagccagctt ggcagcgtcg
     1561 aggtcgaccg cgaggcgttc ccaggcgagt cggcgcgctg ccagcggtgc catcacgctg
     1621 tcgacgccgg ccagcgtgac gccgcgcagg atgaacggca tcacggtcgc cggcaggtcg
     1681 aagccctgcg ccagcccgca ggccgcgacc acgccgccgt agcgcgtctg cgcgagcgca
     1741 ttgaccagcg tgtggctgcc caccgcatcg accaccgcgg cccagcgctc cttttgcagc
     1801 ggcttgccgg gcgcactcaa ctcggcgcga tcgatcacgc cggcagcccc aagcgcatgc
     1861 aaatagccgg cctcacccgg cttgccggtg gcggcgatga cgcggtgccc gagccggccc
     1921 agcaaggcca ccgcgacgct gccgacgccg ccggtcgcgc cggtcaccag cacctcgccg
     1981 tcgcccgcct tgacgccatg gcgctcgagc gccagcacgc acagcatcgc ggtgtagccg
     2041 gccgtgccga tcgccatcgc ctgcaacgtg gagaacgccg ccggcagcgg caccagccag
     2101 tcgcccatca ggcgcgcgcg ctcggccagg caaccccagc gggtttcacc gacgcc
//
LOCUS       DATMJB010000564         2625 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_497256, whole genome shotgun sequence.
ACCESSION   DATMJB010000564 DATMJB010000000
VERSION     DATMJB010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497256"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1347)
                     /locus_tag="VNU48_12080"
     CDS             complement(85..1347)
                     /locus_tag="VNU48_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWI12060.1"
                     /translation="MRTSLLHAPRHQRGTTLIEALIAFLVLSLGLLAVARVQTQLRQN
                     ADVARQRSEAVRLAQEDLESLRAFAVLAAAPGARSFEAIADDAHTVESAGGSYLVTRR
                     VDAAATPHAKNVSVGVAWTDRSGGAQQFGLASVIAGTAPAFSGALGLARTAAAKGALA
                     RSARIPLGAKDLGDGRSAFKPVGNGTLAFVFDNRSGALTGRCAGVDPAIATRDLTSAS
                     LGACDANVGQLLSGVVRFSMTSPPDAAQGNELPAAFTVALALIGGPYPIAPSCGVEAM
                     KTVSYTADGSLHVEAVPVGALAASLGLPGWTETGERFAAYHCAVYPSANGRWSGQTTL
                     VPSGWTIGTGASDRRVCRYSSDLDASGAIDTNLEHPASYTNVGASLANQNFLVIKGSE
                     ACPVGAPTQVAGNNADVYVDLSTVAHQP"
     gene            complement(1359..>2625)
                     /locus_tag="VNU48_12085"
     CDS             complement(1359..>2625)
                     /locus_tag="VNU48_12085"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HWI12061.1"
                     /translation="SSRPIQSQRGAAALVVTMLLLFAMLLVAVFANRHLVFEQRGAAN
                     QARSTLAFEAAEAGLEWAIAQLNTNRRIDAACQPSADPAAASFRGRYLVVARGTGVIT
                     PTTWNQAGTATALQAACVRSGDDWTCSCPAQGLPTLPAPTGSVPAAAFVLQFQPVAKA
                     GVVRVVATGCTSLAGACLAGSTTRADASTTLEAMLGLAGGLRSAPAATVTVRGSFDAG
                     NATLGLHNVDPATGLSVDAGGTINAGQARLTTPAGAPKAGALVGNDSALAALDPQRLF
                     AAYFGVDKTHWQGQPAVTRLSCTADCGAALAAAIAAAPDTALIQVDGDLTLAGPLTLG
                     SAQRPVAIVAGGAARLDGAVAITGLLYAGALQWTNTAGGAFVRGALVSEAGYQGNGAP
                     ELFYDSDVLDLLKQRAGSFARVNGSWRDF"
BASE COUNT          345 a          923 c         1005 g          352 t
ORIGIN      
        1 ccacgcgcgg gttcggctcg gacaggccga tcgcgtgctg cgccagcgcc agcgcctcgt
       61 gcatcggaga gggaaacgga tcgctcatgg ttggtgggcc acggtgctca ggtcgacgta
      121 cacatccgca ttgttgccgg caacctgcgt cggcgcgccg accgggcagg cctcgctgcc
      181 cttgatcacc agaaagttct ggttcgccag gctggcgccg acattcgtgt acgacgcggg
      241 gtgctcgagg ttggtgtcga tcgcgccgct cgcatcgagg tcgctgctgt agcggcacac
      301 gcgccggtcg ctcgcgccgg tgccgatcgt ccagccgctg ggcacgagcg tcgtctgccc
      361 cgaccaccgg ccgttggccg acgggtagac cgcgcagtgg taggcggcga agcgctcgcc
      421 ggtctcggtc cagcccggca ggccgagcga tgcggccagc gcgccgaccg gcaccgcctc
      481 gacatgcagg ctgccgtcgg ctgtgtacga cacggtcttc atcgcctcga cgccgcacga
      541 aggcgcgatc gggtacgggc cgccgatcag cgcgagcgcg accgtgaagg ccgccggcag
      601 ttcgttgccc tgggccgcgt cgggcggcga ggtcatggag aagcgcacga cgccgctgag
      661 cagctggccg acgttggcgt cgcacgcgcc caggctcgcc gaggtgaggt cgcgtgtcgc
      721 gatggcgggg tcgacgccgg cgcagcggcc ggtcagcgcg ccggagcggt tgtcgaagac
      781 gaacgcgagc gtgccgttgc cgaccggctt gaaggcactt cggccatcgc ccaggtcctt
      841 cgcgcccagc gggatgcgtg cggagcgcgc caacgcaccc ttggccgccg cagtgcgcgc
      901 cagaccgagc gcgccgctga acgccggcgc ggtgccggcg atgaccgagg ccagcccgaa
      961 ctgttgcgcg ccgccgctgc ggtcggtcca ggcgaccccc acgctcacgt tcttcgcatg
     1021 cggcgtcgcg gcggcgtcga ctcggcgcgt gacgaggtag ctgccgccgg ccgactccac
     1081 cgtgtgcgcg tcgtcggcga tcgcctcgaa cgagcgggca ccgggcgccg cagcgagcac
     1141 cgcgaacgcg cgcagcgatt cgagatcctc ctgggccagc cgcaccgcct cggagcgctg
     1201 gcgcgccacg tcggcgttct ggcgcagctg cgtctgcacg cgggcgacgg cgagcagccc
     1261 caggctcagc accaggaacg cgatcagcgc ctcgatcagc gtggtgccgc gctggtggcg
     1321 cggtgcatgc aacagggaag tgcgcatcgc tcgtctcctc agaagtcgcg ccagctgccg
     1381 ttgacgcgcg cgaaactgcc ggcgcgctgc ttcagcagat cgagcacgtc gctgtcgtag
     1441 aacagctccg gcgcgccgtt gccctggtag ccggcttcgg acaccagcgc gccgcgcacg
     1501 aacgcgccgc cggcggtgtt ggtccattgc agcgcaccgg cgtacagcag accggtgatc
     1561 gcgacggcgc cgtcgaggcg ggccgcgccg ccggcgacga tcgcgaccgg ccgctgcgcc
     1621 gagccgagtg tcagcgggcc ggccagcgtc agatcgccgt cgacctggat cagcgcggtg
     1681 tcgggtgcgg cggctatcgc cgcggcgagg gcggcgccgc agtcggccgt gcagctcagg
     1741 cgcgtgacgg ccggctggcc ttgccaatgg gtcttgtcga cgccgaagta cgccgcgaac
     1801 aggcgctgcg ggtcgagtgc ggcgagcgcg ctgtcgttgc cgaccagggc gccggccttc
     1861 ggcgcgccgg ccggtgtcgt cagacgcgcc tgaccggcgt tgatcgtgcc gccggcgtcg
     1921 accgagaggc cggtcgcggg atcgacgttg tgcaggccga gcgtcgcgtt gcctgcatcg
     1981 aagctgccgc gcaccgtgac cgtggccgcc ggcgcgctgc gcaggccgcc ggccaggccc
     2041 agcatggctt cgagcgtcgt gctggcgtcg gcacgcgtcg tcgagcccgc gaggcaggcg
     2101 ccggcgaggc tggtgcaacc ggtggccacg actcgcacga cgcctgcctt cgcgacgggc
     2161 tggaattgca gcacgaaggc ggcggccggc accgagcccg tgggggccgg caacgtgggc
     2221 agcccctgcg ccgggcagct gcaggtccag tcgtcgccgc tgcggacgca ggcggcttgc
     2281 agcgcggtcg ctgtgccggc ctggttccag gtcgtcggcg tgatcacacc cgtgccgcgc
     2341 gcgacgacga ggtagcggcc gcggaacgac gcggcggccg ggtcggcgct gggctggcag
     2401 gcggcgtcga tgcggcggtt cgtgttgagt tgtgcgattg cccattcgag cccggcctcg
     2461 gcggcctcga aggccagggt cgagcgggcc tggttggcgg cgccgcgctg ctcgaacacg
     2521 agatggcggt tcgcgaacac cgcgaccagc agcatcgcga acagcaacag catcgtgacc
     2581 accagcgcgg cggcgccgcg ctgggattgg atcgggcggg acgac
//
LOCUS       DATMJB010000565         2366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_556526, whole genome shotgun sequence.
ACCESSION   DATMJB010000565 DATMJB010000000
VERSION     DATMJB010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556526"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1781
                     /locus_tag="VNU48_12090"
     CDS             174..1781
                     /locus_tag="VNU48_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWI12062.1"
                     /translation="MSSTSAALPAAGAAKPAPAAPARPSAYAPLTGGQLVLGTVALSL
                     ATFMNVLDSSIANVSIPAISGDLGVSPSQGTWVITSFAVANAISVPLTGWLTQRFGQV
                     RLFTMSVILFVIASWLCGLAPNMPMLIAFRVLQGFVAGPMIPLSQTLLLASYPAAKAG
                     TAMAMWAMTVLVAPVAGPLLGGWITDNISWPWIFYINIPVGIVAAALTWSIYRKRDPG
                     PRRVPLDYVGLGLLVLFVGTMQIMIDKGKELDWFNSGQIVVLAVTASVSFLFFLAWEL
                     TEKHPVVDLRLFARRNFSMGTASLSIAYGLFFGNVVLLPLWLQQYMGYTATWAGLATA
                     PVGILAIVLSPWVGKNVSKIDPRKLATVSFIGFGVVLWMRSNFNTQADFTTILVPTVL
                     QGAAMAFFFIPLQAIVFSGLTPDKLPSAAGLSNFVRITAGAVGTSLFTTLWENRATLH
                     HAQLTEAINATNNAATQTMGQLAAGGFNSDQVAANLNRMIDQQAYTMAATDVFYMSSL
                     LFFALVAVIWLARPKPGGAAAGGGGAH"
     gene            complement(1824..>2366)
                     /locus_tag="VNU48_12095"
     CDS             complement(1824..>2366)
                     /locus_tag="VNU48_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HWI12063.1"
                     /translation="GANETSRYYSEPQLAVRGTLTLAGRAVAVQGRAWLDHEWSDAFI
                     PRQAVGWDWIGMNLDDGSALTAFRLRRADGGSVYAGGSFRRGGEAVRAFGPDEVRFTP
                     GRVWVSAATRARYPVQWQIETPAGRFGVAALLDDQELDSRGSTGAIYWEGLSELLDAS
                     GRRIGRGYLELTGYAAPLRL"
BASE COUNT          312 a          845 c          826 g          383 t
ORIGIN      
        1 ggcgagcgcg atcgcggtgg cgagcagcgc cgcgacgaag ccgccggccg gcgccgcgac
       61 cgccgcggtg gccagcccga ccgccgccgc ggcgtcggtg gcgacgatcg ccggcgcgac
      121 gacggtccag cccgtcgccg atctgaagtg atgatgcgcc ggagcgcctg accatgtcct
      181 cgacctctgc cgcactgccg gccgccggtg cggccaagcc ggcgccggcc gcgccggcac
      241 gacccagcgc gtacgcaccg ctgaccggcg gccagctggt gctgggcacc gtcgcgctgt
      301 cgctcgcgac cttcatgaac gtgctcgaca gctcgatcgc caacgtctcg atcccggcga
      361 tctcgggcga cctgggcgtg agcccgtcgc agggtacctg ggtcatcacc tcgttcgcgg
      421 tggcgaacgc gatctcggtg ccgctgaccg gctggctcac gcagcgcttc ggtcaggtgc
      481 gcctgttcac gatgagcgtg atcctgttcg tgatcgcctc gtggctgtgc gggctggcgc
      541 cgaacatgcc gatgctgatt gcgttccggg tgctgcaggg cttcgtcgcc ggcccgatga
      601 tcccgctgtc gcagaccttg ctgctggcca gctacccggc cgcgaaggcc ggcaccgcga
      661 tggcgatgtg ggcgatgacg gtgctggtcg cgccggtggc cgggccgctg ctcggcggct
      721 ggatcaccga caacatctcg tggccgtgga tcttctacat caacattccg gtgggtatcg
      781 tcgcggccgc cctgacctgg tcgatctacc gcaagcgcga cccgggcccg cggcgcgtgc
      841 cgctcgacta tgtcggtctc gggctgctgg tgctgttcgt cggcacgatg cagatcatga
      901 tcgacaaggg caaggagctc gactggttca actcgggcca gatcgtcgtc ctggccgtga
      961 ccgcgagcgt gtccttcctg ttcttcctcg cctgggagct caccgagaag cacccggtcg
     1021 tcgacctgcg cctgttcgcg cggcgcaatt tctcgatggg caccgcgtca ctgtcgatcg
     1081 cgtatgggct gttcttcggc aacgtcgtgc tgctgccgct gtggctgcag cagtacatgg
     1141 gctacaccgc cacctgggcg ggcctggcga cggcgccggt gggcatcctc gcgatcgtgc
     1201 tgtcgccgtg ggtcggcaag aacgtcagca agatcgaccc gcgcaagctc gcgaccgtgt
     1261 ccttcatcgg cttcggcgtc gtgctgtgga tgcgctcgaa cttcaacacg caggccgact
     1321 tcacgaccat cctggttccg acggtgctgc agggcgcggc gatggcgttc ttcttcatcc
     1381 cgctgcaggc gatcgtgttc tcggggctga cgcccgacaa gctgccgtcg gccgcggggc
     1441 tgtcgaactt cgtgcgcatc accgcgggcg cggtcggcac ctcgctgttc acgacgctgt
     1501 gggagaaccg cgccacgctg caccacgcgc agctgaccga ggcgatcaac gcgaccaaca
     1561 acgccgcgac gcagaccatg ggccagctcg ccgccggcgg cttcaacagc gaccaggtcg
     1621 cggcgaacct gaatcgcatg atcgaccagc aggcctacac gatggcggcg accgacgtgt
     1681 tctacatgtc ctcgctgctg ttcttcgcgc tcgtcgcggt gatctggctc gcgcggccca
     1741 agcccggcgg cgcggcggcc ggcggcggag gggcgcattg atggagcccc tcggcccggc
     1801 gctgggcccg ccgcgctgaa tttctacaga cgcagcggcg cggcgtagcc ggtcagttcg
     1861 aggtagccgc ggccgatgcg tcggccggag gcgtcgagca gctcgctcag gccttcccag
     1921 tagatcgcgc cggtgctgcc gcggctgtcg agctcctgat cgtcgagcag cgcggcgaca
     1981 ccgaagcggc ccgccggcgt ctcgatctgc cactgcaccg ggtagcgcgc gcgcgtcgcc
     2041 gcgctgaccc agacgcggcc cggtgtgaag cgcacctcgt ccgggccgaa ggcgcgcacc
     2101 gcctcgccgc cacgccgaaa gctgccgccg gcgtacacgc tgccgccgtc ggcgcggcgc
     2161 aggcggaacg cggtgagcgc gctgccgtcg tccaggttca tgccgatcca gtcccagccg
     2221 acggcctgcc gcgggatgaa cgcgtcgctc cattcgtgat cgagccaggc gcggccctgc
     2281 acggcgacgg cgcggccggc cagcgtcaac gtgccccgca ccgcgagctg cggctcgctg
     2341 tagtagcggc tggtctcatt ggcgcc
//
LOCUS       DATMJB010000566         4363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_588038, whole genome shotgun sequence.
ACCESSION   DATMJB010000566 DATMJB010000000
VERSION     DATMJB010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588038"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VNU48_12100"
     CDS             <1..414
                     /locus_tag="VNU48_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWI12064.1"
                     /translation="CARLGVGTAASKLGISVDILAPGKRGCPYHFHYAQEEAFVVLEG
                     EGQLRVAGELLTLRAGDVVFIPAGPQYPHQIVNSSAAPLKYLSISTRESPEVVEYPDS
                     GKYLATGTGPLGDTTHRFSRMNRSADDLDYWEGEP"
     gene            477..4166
                     /locus_tag="VNU48_12105"
     CDS             477..4166
                     /locus_tag="VNU48_12105"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HWI12065.1"
                     /translation="MVIWIRRFAWGVAALLLLWLVLWLAVPALLKWQLPLRASEALGR
                     PVTIGDVSFRPWNLDLTVKELAVAAQPAGAEPLLRIGQVHANLSAASLFRFAPVIEAL
                     EIDALRLRVARTGDGHYDIDDLIARFASRPDAKPASEPARFALYNLQVRDAQLRFDDR
                     PVNRVHQIEALQLTLPFISDLPAQVDIKVEPRLAFKLNGTPFDTGAQATPFAQTKSGD
                     LKLAMADLDLGPYLGYLPATLPVRITRGSVSADLALNFTVPPGAAPLATLKGKVGAKD
                     LVLTDAAGAPLLAWQQLQLGLRDVQPLARKLALDSLRIDGAQLHATRDAAGGINLLQL
                     TAPPGAAKADEPAAAASAAARPPAPTAWQLSLEALDLTDARVLWTDAAVKPTATLQLD
                     GLSVSAKQVRWPLTQAFPVAIKGALHTQAAGAPALAEFWAEGPVTDHDAKLALKVTSV
                     SLAAFSPYLAQTLVPSVDGQLAAQARLDWSGAVDAPRLQLTLDSLALDSLRVREGGRR
                     GVPDAVALKQLALADVTVDLLARTVALGSVRLVQPAVQLVRDKDGRLNVQAWIVAGPP
                     APAAVRAAPVAPAWQLSLKDFLLEDGQLRFADSIVRRGVAEPVRAELSSLRVALQNLQ
                     WHGERAVPPANVQISARVGGITRDRQRPSGVVDFKGRVGMQPLLASGQLRVERVPVQL
                     FKPYFAELAQLTLVRAEVGYTGSVNLREWPAGLEASAAGDVLLGDVHIATLPGPGAPL
                     NLATADELLSWQAFALKGLKVALKPKARPQVEIGEASLSDFYSRLVVTEQGRFNLQEV
                     AGAPADAASAPPVAAATPPASAPGGDLPIDLKIGATRLINGRVDFTDHFERPNYSAAL
                     TELNGELGGFSSGSREMATLALRGRAEGTALLEISGLVNPSVKPLALDIRAKATDLEL
                     APLSPYAGKYAGYAIERGKLTMDVAYKIDADGKLEAKNQVVLNQLSFGDKIESKDATK
                     LPVLLAVALLKDRNGVIDINLPVSGSINDPQFSVGGIIWKVILNLLTKALTAPFSLFA
                     GGASDDLSLVEFRPGTAQVAPAGASAIDKVAKALSERPSLKMTVTGSADPASEREAFQ
                     AAELESRLVAEQRREGLRAGAAATAPGAPASAPVALSGDERARLLKQVYKDTELPNKP
                     RNLIGFAKDIPGPEMEALLKARAPVTTEAMRALALQRGIAVRDALIAKGLPSERLFLA
                     APKLRVSGEDNAAWTPRVQLSLSTN"
BASE COUNT          654 a         1526 c         1567 g          616 t
ORIGIN      
        1 tgcgcacgcc tgggcgtcgg caccgcggcc agcaagctcg gcatcagcgt cgacatcctc
       61 gcgcccggca agcgcggctg cccgtaccac ttccattacg cgcaggagga ggctttcgtc
      121 gtgctcgagg gcgagggcca gctgcgcgtc gcgggtgagc tgctgacgct gcgcgcgggc
      181 gacgtggtgt tcatcccggc cggcccgcaa tacccgcacc agatcgtcaa cagctcggcc
      241 gcgccgttga agtacctgtc gatcagcacg cgcgagagcc cggaggtcgt cgagtacccg
      301 gattcgggca agtacctcgc gacgggcacc ggtccgttgg gcgacacgac gcatcggttc
      361 tcgcgcatga accgctcggc cgacgacctg gactactggg aaggcgagcc ctgagccgct
      421 gtcggatgtg acaggatggc acccacacga caggtttcgc tgaacaggga aggtggatgg
      481 tgatctggat caggcggttc gcgtggggcg tggcggcgct gctgctgctg tggctggtcc
      541 tgtggctcgc cgtgccggcg ctgctgaagt ggcagctgcc gttgcgcgcc agcgaggcgc
      601 tcggtcgccc ggtgacgatc ggcgatgtga gcttccggcc ctggaacctg gacctcacgg
      661 tcaaggagct cgcggttgcc gcgcagccgg ccggcgccga gccgctgctc aggatcgggc
      721 aggtgcacgc gaacctgtcg gcggcgtcgc tgttccggtt cgcgccggtg atcgaggcgc
      781 tcgagatcga cgcgctgcgc ctgcgcgtgg cgcgcaccgg cgacggtcac tacgacatcg
      841 acgacctgat cgcgcgcttc gcgtccaggc ccgatgcgaa acctgcgagc gagccggcgc
      901 gcttcgcgct gtacaacctg caggtgcgcg acgcgcagct gcgcttcgac gaccggccgg
      961 tgaaccgcgt ccaccagatc gaggcgctgc agctcacgct gccgttcatc tcggacttgc
     1021 cggcgcaggt cgacatcaag gtcgagccgc ggctcgcgtt caagctcaac ggcacgccct
     1081 tcgacaccgg cgcgcaggcc acgccgttcg cgcagaccaa gtccggcgat ctgaagctgg
     1141 cgatggccga cctcgatctc gggccctacc tcggctacct gccggccacg ctgccggtgc
     1201 gcatcacacg cggcagcgtc tcggccgacc tggccctcaa cttcaccgtg ccgcccggcg
     1261 ccgcgccgct ggcgacgctg aagggcaagg tcggcgcgaa ggatctcgtg ctgaccgacg
     1321 cggccggtgc gccgctgctg gcctggcagc aactgcagct cgggctgcgc gacgtgcagc
     1381 cgctggcgcg caagctcgcg ctcgacagcc tgcgcatcga cggagcgcag ctgcacgcga
     1441 cgcgtgacgc agccggcggg atcaacctgc tgcagctcac tgcgccgccg ggcgccgcga
     1501 aggccgacga gcccgcggcg gcagcatcgg cagcggcgcg gccgccggcg ccgaccgcgt
     1561 ggcagttgag tctcgaggcg ctggacctga ccgacgcgcg cgtgctctgg accgatgccg
     1621 ccgtgaagcc cactgcgacc ctgcagctcg acggcctgag cgtcagcgcg aagcaggtcc
     1681 gctggccgct gacacaggcg tttcccgtcg cgatcaaggg cgcgttgcac acgcaggccg
     1741 cgggcgcgcc ggcactcgcc gagttctggg ccgagggccc ggtcaccgat cacgacgcga
     1801 agctcgccct caaggtcacg agcgtgtcac tggccgcgtt ttcaccctat ctggcgcaga
     1861 ccttggtgcc cagtgtcgat ggccagcttg ccgcgcaggc ccggctcgac tggtcgggcg
     1921 cggtcgacgc gccgcgcctg cagctcacgc tcgacagcct cgcgctcgac agcctgcgcg
     1981 tgcgcgaggg tggccgccgc ggcgtgccgg acgcggtcgc gctgaagcag ctcgcgctcg
     2041 ccgacgtcac ggtcgacctg ctcgcgcgca ccgtcgcgct cggcagcgtc aggctcgtgc
     2101 aaccggcggt ccaactcgtg cgcgacaagg acgggcgcct gaacgtgcag gcttggatcg
     2161 tcgccggccc gcccgcgccc gccgcggtgc gcgccgcgcc cgtggcgccg gcctggcaac
     2221 tgagcctgaa ggactttctg ctcgaggacg ggcagctgcg cttcgccgac tcgatcgtgc
     2281 ggcgcggtgt cgccgagccg gtgcgcgccg agttgtcgag cctgcgcgtc gcgctgcaga
     2341 acctgcagtg gcacggcgag cgcgcggtgc cgccggcgaa cgtccagatc agcgcgcgcg
     2401 tcggtggcat cacccgcgat cggcagcgcc cgagcggcgt ggtcgacttc aagggccgcg
     2461 tgggcatgca gccgctgctg gcgagcggac agttgcgcgt cgagcgtgtc ccggtgcagt
     2521 tgttcaagcc gtacttcgcc gagctggcgc agctgacgct ggtgcgcgcc gaggtcggct
     2581 acaccggcag cgtgaacctg cgcgagtggc ccgcggggct ggaggcttct gccgcgggcg
     2641 acgtgctgct cggcgacgtg cacatcgcaa cgctgccggg gcccggcgcg ccgctgaacc
     2701 tcgccaccgc ggacgagttg ctcagctggc aggccttcgc gctgaagggc ctgaaggtcg
     2761 cgctgaagcc gaaggcgcga ccacaggtcg agatcggcga ggcctcgctg agcgacttct
     2821 actcgcgcct ggtggtcacc gagcagggcc gcttcaacct gcaggaggtt gccggcgcgc
     2881 cggccgatgc agcgtcggcg ccgccggtcg ccgcggccac gccacccgca tcagcgcctg
     2941 gcggcgatct gccgatcgac ctgaagatcg gcgcgaccag gctgatcaac ggccgcgtcg
     3001 atttcaccga ccacttcgag cgcccgaact acagcgcggc gctgaccgag ctgaacggcg
     3061 agctcggcgg cttcagctcg ggcagccgcg agatggccac cctcgcgctg cgcggccgcg
     3121 ccgagggcac cgcgctgctg gagatcagcg gcctggtgaa cccgagcgtg aagccgctcg
     3181 cgctcgacat ccgcgcgaag gccaccgacc tcgaactggc gccgctgtcg ccctacgccg
     3241 gcaagtacgc cggctacgcg atcgagcgcg gcaagctgac gatggacgtg gcctacaaga
     3301 tcgatgccga cggcaagctc gaggcgaaga accaggttgt gctgaaccag ctgtccttcg
     3361 gcgacaagat cgagtcgaag gacgcgacca agctgccggt gctgctcgcg gtggcgctgc
     3421 tgaaggaccg caacggcgtg atcgacatca acctgccggt cagcggcagc atcaacgacc
     3481 cgcagttcag cgtcggcggc atcatctgga aggtcatcct gaacctgctg acgaaggcgc
     3541 tgacggcgcc gttctcgctg ttcgccggcg gcgccagcga cgacctgagc ctggtcgagt
     3601 tcaggcccgg cacggcgcag gtggcgccgg ccggcgcgag cgcgatcgac aaggtcgcga
     3661 aggccctgag cgagcggccg tcgctgaaga tgacggtcac cggttcggcc gacccggcct
     3721 cggagcgcga ggcgttccag gccgccgaac tcgagtcgcg cctggtggcc gaacagcgcc
     3781 gcgaaggcct gcgtgccggc gctgcggcaa cggcacccgg cgcgccggcg agcgcgcccg
     3841 tggcgctgag cggcgacgag cgcgcgcggc tgctgaagca ggtctacaag gacaccgagc
     3901 tgccgaacaa gccgcgcaac ctgatcggct tcgcgaagga cattcccgga cccgagatgg
     3961 aggcgctgct gaaggcgcgc gcgccggtca ccaccgaggc gatgcgcgcg ctggcgctgc
     4021 agcgcggcat cgcggtgcgc gatgcgctga tcgccaaggg gctgccgagc gagcggctgt
     4081 tcctggccgc gccgaagctg cgcgtctcgg gcgaggacaa tgcggcgtgg acgccgcgcg
     4141 tgcagctgtc gctgtcgacc aattgatgga caagaattcg gacaccttgg cctataactc
     4201 gtatttgagt gacgtgagct gtacctgttc gggcgaacgc cgatgactct cctaagaagc
     4261 tgcatagcga ccgccgcgtg cttgatcacg acgggatcgg ttcgagcgca acaatccgat
     4321 ggccttgagt tcctcaggcc gtccaaccgc gcatacgtag agg
//
LOCUS       DATMJB010000567         3812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_852705, whole genome shotgun sequence.
ACCESSION   DATMJB010000567 DATMJB010000000
VERSION     DATMJB010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852705"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..426
                     /locus_tag="VNU48_12110"
     CDS             10..426
                     /locus_tag="VNU48_12110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HWI12066.1"
                     /translation="MATLHVDVVSAEGSIFSGEAKFVALPGESGELGILPRHTPLITR
                     IKPGAVRIERADNGEEEFVFVAGGILEVQPGTVTVLADTAIRGRDLDEAKANEAKRLA
                     EDAMKNAKSDIDFAKAQGEFAALAAQIAAIRKFRGK"
     gene            complement(435..3110)
                     /gene="mdeB"
                     /locus_tag="VNU48_12115"
     CDS             complement(435..3110)
                     /gene="mdeB"
                     /locus_tag="VNU48_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017330650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate dehydrogenase"
                     /protein_id="HWI12067.1"
                     /translation="MTEPSDSAAAVRHIGAILATTAPDADPTETAEWCEALDALSAAH
                     GPERARFLLDALLVHARRRGLNWLPSMVTPYVNSIALEAQPPFPGDLAMEQRLAALMR
                     WNALAMVMRANRVETGGSAELGGHIASYASAADLFEVGFNHFFRARNDRAGQLGDLVF
                     FQPHSAPGVYARAFLEGRLTADDLAHYRQELSAPARGARGLTSYPHPQLMPDFWQFPT
                     GSMGIGPINAIYQARFMRYLQHRGLLAATRAGTAQRRVWGFFGDGEMDEPESIAALSL
                     AAREQLDNCTFVINCNLQRLDGPVRGNGRIVEELESLFAGAGWRVVKCLWSSDWDVLF
                     ARDHGHALMRAFARTVDGEFQTLSAKDGKFNREMFFGRNPELLALVADLRDEDIDRLR
                     RGGHDAVKIHAAFAAAAAHTGQPTVVLAKTMKGYAMGDTAQGRMTTHSAKKLDEAALL
                     AFRDRFALPLDDAATRELAFCRPAPDSAELKYLHSRRQALGGALPARRTDCAVLPVPP
                     LESTAAFALDAAGKEMSTTMAFVRLLGNLLKDATLGPRIVPIVADEARTFGMAGLFRQ
                     VGIYAPFGQLYQPEDSGSVLSYREAATGQILEEGITEAGAVSSWTAAATAYSTHGLAM
                     LPFYIYYSMFGFQRVGDLIWAAADQRARGFLIGATSGRTTLGGEGLQHQDGSSHLIAA
                     TVPNCKAYDPAFAGELAVIVDHGMREMLVEQRDVFYYVTAMNENAAQPSLPADAHEGV
                     IRGLYCCRRAATASGPRVQLLGSGAILGEALKAADALAAEHGVAADVWSVTSFSELAR
                     DGVDAETAWRHGKADTIDAYLTRQIGPTEGPVVIATDYVRAVPEQIRAFLPAGRRCVT
                     LGTDGFGRSDTRAALRRHFEVDAAAIVAAALRAMR"
     gene            complement(3198..>3812)
                     /locus_tag="VNU48_12120"
     CDS             complement(3198..>3812)
                     /locus_tag="VNU48_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HWI12068.1"
                     /translation="ERAAGPMIGTVLIDLLLTSTLCLAIAQTAAQQSGGRATAVLRSL
                     RGAATNPLPWAIVIGALFAAFDRELPGPIAQIVRMLGDSATPVALFTIGSVLSRAGRH
                     AHARTPLGRYLPVALLKLLLHPALVFGLGSAAIALGAPVPRTGLMVLTLVAALPSASN
                     VSVLAERYGADNGRVTRIIMASTVLAFASFSALAWAFGVGRAGV"
BASE COUNT          561 a         1355 c         1376 g          520 t
ORIGIN      
        1 gagcccgaca tggcaaccct gcatgtagac gtggtctcgg ccgaaggcag catcttctcc
       61 ggcgaggcga agttcgtcgc gctgccgggc gagagcggcg agctcggcat cctgccgcgc
      121 cacacgccgc tgatcacccg catcaagccc ggcgcggtgc gcatcgagcg cgccgacaac
      181 ggcgaggaag agttcgtctt cgtcgccggc ggcatcctcg aagtgcagcc gggcacggtc
      241 accgtgctcg ccgacaccgc gatccgcggt cgcgatctgg acgaagccaa ggccaacgag
      301 gccaagcgtc ttgccgaaga cgcgatgaag aacgccaaga gcgacatcga tttcgcgaag
      361 gcgcagggcg agttcgcggc gctggcggcg cagatcgcgg cgatccgcaa gttccgcggc
      421 aagtaggcag ccagctaacg catcgcgcgc agcgcggcgg cgacgatcgc cgcggcgtcg
      481 acctcgaagt gcctgcgcag cgcggcccgc gtgtcgctgc ggccgaagcc gtcggtgccc
      541 agtgtcacgc agcgccggcc cgccggcagg aaggcgcgga tctgctccgg caccgcgcgc
      601 acgtagtcgg tcgcgatcac gacggggcct tcggtgggcc cgatctggcg cgtcaggtag
      661 gcgtcgatcg tgtcggcctt tccatgccgc cacgcggttt cggcgtcgac gccatcgcgt
      721 gccagttcgc tgaagctggt gacgctccag acatcggcgg cgacgccgtg ctcggccgcc
      781 agcgcgtcgg cggccttcag cgcttcgccg aggatcgcgc cggacccgag cagttgcacg
      841 cgcggccccg atgccgtcgc cgcccggcga caacagtaca ggccgcggat cacgccctcg
      901 tgcgcgtcgg ccggcagcga cggctgcgcg gcgttctcgt tcatcgccgt cacgtagtag
      961 aagacgtcgc gctgctcgac cagcatctcg cgcatgccgt ggtcgacgat caccgccagt
     1021 tcaccggcga acgccgggtc gtaggccttg cagttcggca ccgtcgcggc gatcaggtga
     1081 ctgctgccgt cctggtgctg caggccttcg ccgccgagcg tcgtgcgccc cgaggtcgcg
     1141 ccgatcagga agccgcgtgc gcgctggtcg gcggcggccc agatcaggtc gccgacgcgt
     1201 tggaagccga acatgctgta gtagatgtag aacggcagca tcgccaggcc gtgggtcgag
     1261 taagccgtcg cggccgcggt ccacgacgag accgcgccgg cctcggtgat gccttcctcg
     1321 aggatctgtc cggtcgcggc ctcgcggtac gacagcaccg agcccgagtc ctccggctgg
     1381 tagagctggc cgaacggcgc gtagatgccg acctggcgga acagcccggc catgccgaag
     1441 gtgcgcgcct cgtcggcgac gatcggcacg atgcgcgggc ccagcgtcgc gtccttcagc
     1501 aggttgccga gcaggcgcac gaaggccatc gtcgtgctca tctccttgcc cgcggcgtcg
     1561 agcgcgaagg ccgccgtcga ttcgagcggc ggcacaggca gcaccgcgca atcggtgcgc
     1621 cgcgccggca gcgcaccgcc cagcgcctgg cgccggctgt gcaggtactt cagctcggcg
     1681 ctgtccggcg cgggccggca gaacgcgagt tcccgcgtcg ccgcgtcgtc gagcggtagc
     1741 gcgaagcgat cgcggaaggc gagcagtgcg gcctcgtcga gcttcttcgc cgagtgcgtc
     1801 gtcatgcggc cctgcgcggt gtcgcccatc gcgtagccct tcatcgtctt cgcgagcacg
     1861 acggtgggct ggccggtgtg cgccgccgct gccgcgaagg cggcgtggat cttcaccgcg
     1921 tcgtggccgc cgcggcgcag ccggtcgatg tcctcgtcgc gcaggtcggc gacgagcgcg
     1981 agcagctcgg ggttgcggcc gaagaacatc tcgcggttga acttgccgtc cttcgccgac
     2041 aaggtctgaa actcgccgtc gacggtacgc gcgaacgcgc gcatcagcgc gtggccgtgg
     2101 tcgcgcgcga acagcacgtc ccagtcggag ctccacaggc acttgacgac gcgccagccg
     2161 gcgccggcga acagcgactc gagctcctcg acgatgcggc cgttgccgcg caccgggcca
     2221 tcgaggcgct gcaggttgca gttgatcacg aaggtgcagt tgtcgagctg ctcgcgcgcg
     2281 gccagagaca gcgcggcgat cgattcgggc tcgtccatct cgccgtcgcc gaagaagccc
     2341 cagacccgtc gttgcgccgt tcccgcgcgc gtcgcagcca gcaggccgcg gtgctgcagg
     2401 tagcgcatga agcgcgcctg gtagatcgca ttgatcgggc cgatgcccat cgagccggtc
     2461 gggaactgcc agaagtccgg catcagctgc gggtgcgggt acgaggtgag gcctcgcgcg
     2521 ccgcgcgccg gcgcgctcag ttcctggcgg tagtgcgcga gatcgtcggc cgtcagccgg
     2581 ccttcgagga acgctcgcgc gtagacgccg ggcgccgaat gcggctggaa gaacaccagg
     2641 tcgccgagct gcccggcgcg gtcgttgcgc gcgcggaaaa aatggttgaa gccgacctcg
     2701 aataggtccg ccgccgacgc atagctcgcg atgtgcccgc cgagctcggc cgagccgccg
     2761 gtctcgacgc ggttcgcgcg catcaccatc gccagcgcgt tccagcgcat cagcgcggcc
     2821 aggcgctgct ccatcgcgag gtcgccgggg aacggcggct gcgcctccag cgcgatgctg
     2881 ttgacgtagg gcgtgaccat cgacggcagc cagttcaggc cgcgccggcg cgcatgcacg
     2941 agcaacgcat cgagcaggaa ccgcgcacgc tcggggccgt gcgccgcgct cagcgcatcg
     3001 agtgcctcgc accactcggc ggtttcggtc gggtcggcgt cgggcgcggt cgtcgcgagg
     3061 atggcgccga tgtggcgcac cgctgcggcg gaatcggagg gctcggtcat cgcccgattc
     3121 tgccggctcg gcgcaggcgt gtcaggccgc gaacccggtc cgcccttcgc ccggcagcgg
     3181 cgtgtcggca accggcgtca gacgcccgcg cggccgacgc cgaaggccca cgccagcgcc
     3241 gagaagctcg cgaacgcgag cacggtcgag gccatgatga tgcgcgtgac gcggccgttg
     3301 tcggcgccgt agcgttctgc cagcaccgag acgttgctcg cgctcggcag cgccgcgacc
     3361 agggtcagca ccatcagccc ggtccttggc accggcgcgc cgagcgcgat ggcggcactg
     3421 cccaggccga acaccagcgc cgggtgcagc agcagcttca gcagcgccac cggcaggtag
     3481 cggcccagcg gcgtgcgcgc gtgcgcatgc cggccggcgc gcgacagcac cgagccgatc
     3541 gtgaacagcg ccaccggcgt cgccgagtcg cccagcatgc gcacgatctg cgcgatcggt
     3601 cccggcagtt cgcggtcgaa cgcagcgaac agcgcgccga tcacgatcgc ccacggcagc
     3661 gggttcgtcg ccgcgccgcg cagcgagcgc agcacagcgg tcgcacggcc gcccgactgt
     3721 tgcgcagcgg tctgcgcgat cgccaggcac agcgtgctgg tcagcagcag gtcgatcagc
     3781 acggtgccga tcatcgggcc cgcggcgcgt tc
//
LOCUS       DATMJB010000568         2080 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_735189, whole genome shotgun sequence.
ACCESSION   DATMJB010000568 DATMJB010000000
VERSION     DATMJB010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735189"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VNU48_12125"
     CDS             <1..1164
                     /locus_tag="VNU48_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI12069.1"
                     /translation="KKAVPAFHIPGIVHFNQAIAQGRGKTFKPATVGPDDIAVLQYTG
                     GTTGVSKGAVLLHRNLVANILQSEAWYQPALKKIPAGQQVVTICALPIYHIFGFNTNM
                     MLAMRMGGANILIANPRDLPAMFKELKGEIFHSFPAVNTLFNAMAHHPEFGSVDWSGL
                     VISVGGGMAVQSATAKLWLEKTGCPIVEGYGLSETSPSATCNPVDSTAYSGNIGLPMP
                     NTELKLLDDDGNEVPLGTPGEIAIKGPQVMAGYWQRPDETAKVMTADGFFRSGDIGVV
                     DERGYFKIVDRKKDMILVSGFNVYPNEVEDVVTQMPGILECAAVGIPDAKAGEAVKLV
                     IVRSNPAVTEADVRAYCEANLTGYKRPKVIEFRTELPKTNVGKILRRELRDKA"
     gene            1266..1748
                     /locus_tag="VNU48_12130"
     CDS             1266..1748
                     /locus_tag="VNU48_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12070.1"
                     /translation="MIWAKTDAGRTEMQARALVKERAQRNLLLVIDGIKTEEMLLANL
                     AGINADDFAALQSLGLIVPAASRSGGSVTARSGAPAPVAAAAAAEPAALDYGQFTAAL
                     TQMISKELGLRGFVLTLAVEKAGTIEELQAVAQRTLAQIAERKGEAVAAAARRTLYGN
                     "
     gene            1799..>2080
                     /locus_tag="VNU48_12135"
     CDS             1799..>2080
                     /locus_tag="VNU48_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HWI12071.1"
                     /translation="MSRPPIAIVSAMHEELRALLPLLHDRQSTRLAGRDFHQGTIHGR
                     PVVLVLSGIGKVAAATTAVLLMHEFGARALVFTGVAGGLHRDVKVGDVVV"
BASE COUNT          363 a          711 c          710 g          296 t
ORIGIN      
        1 aagaaggcgg tgccggcgtt ccacatcccc ggcatcgtgc acttcaacca ggcgatcgca
       61 cagggccgcg gcaagacctt caagccggcc acggtcgggc cggacgacat cgcggtgctg
      121 cagtacaccg gcggcacgac cggcgtcagc aagggcgcgg tgctgctgca ccgcaacctg
      181 gtcgcgaaca tcctgcagtc cgaggcctgg taccagccgg cgctgaagaa gatcccggcc
      241 ggccagcagg tcgtgacgat ctgcgcgctg ccgatctatc acatcttcgg cttcaacacg
      301 aacatgatgc tggcgatgcg catgggcggc gcgaacatcc tgatcgcgaa cccgcgcgac
      361 ctgccggcga tgttcaagga actgaagggc gagatcttcc acagcttccc ggccgtcaac
      421 acgctgttca acgcgatggc gcaccacccc gagttcggca gcgtcgactg gagcggcctg
      481 gtgatctcgg tcggcggcgg catggcggtg cagagcgcga ccgcgaagtt gtggctcgag
      541 aagaccggct gcccgatcgt cgagggttac ggcctgtccg agacctcgcc gtcggcgacc
      601 tgcaacccgg tcgacagcac cgcctacagc ggcaacatcg gtctgccgat gcccaacacc
      661 gagctgaagc tgctcgacga cgacggcaac gaggtgccgc tgggcacgcc gggcgagatc
      721 gcgatcaagg ggccgcaggt gatggccggc tactggcagc gccccgacga gaccgccaaa
      781 gtgatgaccg ccgacggctt cttccgcagc ggcgacatcg gcgtggtcga cgagcgcggc
      841 tacttcaaga tcgtggaccg caagaaggac atgatcctgg tcagcggctt caacgtctac
      901 ccgaacgagg tcgaggacgt ggtcacgcag atgcccggca tcctcgaatg cgcggcggtc
      961 ggcatccccg acgcgaaggc cggcgaggcc gtcaagctgg tgatcgtgcg cagcaacccg
     1021 gccgtcaccg aagccgacgt gcgcgcctac tgcgaggcca acctgaccgg ctacaagcgg
     1081 cccaaggtga tcgagttccg caccgaactg ccgaagacca acgtcggcaa gatcctgcgc
     1141 cgcgagttgc gcgacaaagc gtgagcgtgc gttctgccac ggccaccgtg cggcggtact
     1201 gatacattgc ttggtcgcgc ggtccgatgc aggccgcgcg cgctttcgtc aatgggaact
     1261 catcggtgat ctgggccaag accgacgcgg gacgaaccga gatgcaggcg cgtgcgctcg
     1321 tgaaggagcg cgcgcaacgc aacctgctgc tcgtcatcga cggcatcaag accgaggaga
     1381 tgctgcttgc gaacctcgcc ggcatcaacg ccgatgactt cgccgcgctg cagtcgctcg
     1441 ggctgatcgt gccggcagcg agccgttccg gcggttcggt cacggcccgc agtggcgcgc
     1501 cggccccggt cgccgccgcc gcagcggccg agcccgccgc gctcgactac ggccagttca
     1561 ccgccgcact gacgcagatg atctcgaagg aactcggcct gcgcggcttc gtgctgaccc
     1621 tggcggtcga gaaggccggc acgatcgagg aactgcaggc ggtcgcgcag cgcacgctcg
     1681 cgcagatcgc cgagcgcaag ggcgaggccg tggccgccgc cgcccgacgc acgctgtacg
     1741 gcaactgagc ggtcgccacc gcctcctttc ccagcacccg cggatcgggg tcatcacgat
     1801 gtcgcgccca ccgatcgcca tcgtcagcgc gatgcacgaa gagttgcgcg cgctgctgcc
     1861 gttgctgcac gaccgccagt cgacgcgact ggcaggacgc gacttccatc agggcacgat
     1921 ccacggccgg ccggtggtgc tggtgctgtc gggcatcggc aaggtcgccg cggcgacgac
     1981 cgcggtgctg ctgatgcacg agttcggcgc gcgcgcgctc gtcttcaccg gcgtggccgg
     2041 cggcctgcat cgcgacgtga aggtcggtga cgtggtggtc
//
LOCUS       DATMJB010000569         5400 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_584037, whole genome shotgun sequence.
ACCESSION   DATMJB010000569 DATMJB010000000
VERSION     DATMJB010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584037"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="VNU48_12140"
     CDS             complement(<1..165)
                     /locus_tag="VNU48_12140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWI12072.1"
                     /translation="MEINTALFYIFSGVLLFAAFRVITARTPVYAALYLVLAFFNAAC
                     IWMLLRSEFLA"
     gene            complement(216..716)
                     /gene="nuoI"
                     /locus_tag="VNU48_12145"
     CDS             complement(216..716)
                     /gene="nuoI"
                     /locus_tag="VNU48_12145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWI12073.1"
                     /translation="MATAATPVKDFFSSFLLGELFKGMALTGRHFWSRKITVQFPEEK
                     TPLSPRFRGLHALRRYENGEERCIACKLCEAVCPALAITIESEVRDDGSRRTTRYDID
                     LTKCIFCGFCEESCPVDSIVETHIFEYHGEKRGDLYFTKDMLLAVGDRYEKEIAAAKE
                     ADAKYR"
     gene            complement(731..1825)
                     /gene="nuoH"
                     /locus_tag="VNU48_12150"
     CDS             complement(731..1825)
                     /gene="nuoH"
                     /locus_tag="VNU48_12150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWI12074.1"
                     /translation="MIDWLLAVPQSYPASWMVLWSIVKIVALVLPLMICVAYLTLWER
                     KAIGWSQIRPGPNRVGPLGLLQPIADAVKLIFKEIIVPSAANKPLFFLGPVMTIMPAL
                     AAWVVIPFGPQTALSDLNAGLLLLMAITSVEVYGVIIAGWASNSKYAFLGALRASAQM
                     VSYEIAIGFCLVVVLMVSGSLNVTDIVLQQGRGMLAESGWTFLSWNWLPLLPIAVVYF
                     ISGLAETNRHPFDVVEGESEIVAGHMVEYSGMAFAMFFLAEYANMILISAIAVIMFLG
                     GWYAPVTFGLFGMTTPEWIQHTAWFWNWAWLFGKTFCVVTVFLWVRASFPRFRYDQIM
                     RLGWKIFIPVTLVWLVVVGLWIQSPWNIWK"
     gene            complement(1822..4170)
                     /gene="nuoG"
                     /locus_tag="VNU48_12155"
     CDS             complement(1822..4170)
                     /gene="nuoG"
                     /locus_tag="VNU48_12155"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HWI12075.1"
                     /translation="MIEIELDGKKVDVLEGSMIMHAADNAGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPMPACATPVTQGMIVRTKSAKALQAQKGVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGDSASRYREEKRVVFHKNVGPLISMEEMSRCIHCTRCVRFGQEV
                     AGVMELGMIHRGEHSEITTVVGETIDSELSGNMIDVCPVGALTSKPFRYSARTWELSR
                     RKSISPHDSLGANLIVQVKNNQVMRVVPLEVEDINECWIADRDRFSYEALNGPERLTA
                     PMIKQGGAWKTVDWSTALEYVSRGLTQISVDHGPQSVGALGSAHSSVEELHLLAKLMR
                     GLGSENIDHRTRHADFTNVANADAGAPSARWLGTSIAALSTLQRVLVVGSFLRKDHPL
                     FAQRIRQAARKGAKVHSLNAVHDDWLMPMASQCSVAPSGWLQALADIATAVAAGNGAQ
                     APMAGDATDEAKAIAASLLSGERKAILLGNAAAQHPRAAALLALANWIGAQTGASVGY
                     LSEAANSVGAQLVGAMPRAGGLTAGQMLGGPDLKACVLLNVEPTLDAADAAAASAAMH
                     AAEMVVVMSPFKNAAHDVADVLLPIAPFTETSGSFVNAEGRVQSFHGVVKPLGDTRPA
                     WKVLRVLGNLLALDGFEFETSDEVRAEALGDLATIAARLSNQSSASPRDDVGSLRNGL
                     ERIADVPIYATDALVRRAGSLQLTADARAPYASLPPQLWSRLGLSAGDKVRVAQGAAQ
                     AVLPARVDATLAASAVRVPAGHPDTAALGAMFGAITIEKVGP"
     gene            complement(4176..>5400)
                     /gene="nuoF"
                     /locus_tag="VNU48_12160"
     CDS             complement(4176..>5400)
                     /gene="nuoF"
                     /locus_tag="VNU48_12160"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012416731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWI12076.1"
                     /translation="ERLDGYAALKKALDVHPDDLIQLVKDSGLRGRGGAGFPTGLKWS
                     FMPRQFPGAKYLVCNSDEGEPGTCKDRDILMFNPHIVIEGMAIAAYAMGIKVGYNYIH
                     GEIFEVYDRFEEALEEARAAGYLGDNLLGSSFSFQLHAAHGFGAYICGEETALLESLE
                     GKKGQPRFKPPFPASYGLYGKPTTINNTETFAAVPWIIRNGGAPYLAIGKPNNGGTKI
                     FSVVGDVQKPGNYEIPLGTPFSKLLELAGGVRPGRTLKAVIPGGSSAPVLPAAIMNEL
                     TMDYDSIAKAGSMLGSGAVIVMDDSRCMVRSLLRLSYFYMHESCGQCTPCREGTGWLF
                     RMVERIANGQGRMEDIELLNSVSDNIQGRTICALGDAAAMPVRAMIKHFRDEFVHLIE
                     HKTAKVVVAEPAIAA"
BASE COUNT          857 a         1813 c         1816 g          914 t
ORIGIN      
        1 cgcgaggaac tccgaacgca gcagcatcca gatgcaggcg gcgttgaaga acgcgagcac
       61 caggtacagc gcggcataga ccggggtgcg tgcggtgatc acgcggaagg cggcaaacag
      121 cagcacgcct gaaaagatgt agaagagcgc ggtgttgatt tccatgttct tgttcgcgaa
      181 ggctgcatcg ccggcactcg cgcgccggcg gcgggtcagc ggtatttcgc gtccgcctcc
      241 ttggctgcgg cgatttcctt ttcgtagcgg tcaccgaccg ccagcagcat gtccttcgtg
      301 aagtacaggt cgccgcgctt ctcgccgtgg tactcgaaga tgtgggtctc gacgatcgaa
      361 tcgaccgggc agctctcttc gcagaagccg cagaagatgc acttggtcag gtcgatgtcg
      421 tagcgcgtgg tgcggcgcga gccgtcgtcg cgcacctccg attcgatcgt gatcgccagc
      481 gccgggcaca ccgcctcgca cagcttgcag gcgatgcagc gctcttcgcc gttctcgtag
      541 cggcgcagcg cgtgcaggcc gcggaagcgc ggcgacagcg gcgtcttctc ttcggggaac
      601 tgcaccgtga tcttgcgcga ccagaaatgg cgcccggtca gggccatgcc cttgaacagt
      661 tcgccgagca ggaagctcga gaagaagtct ttgacgggtg ttgctgcggt ggccattcga
      721 ttgcccccct tcacttccag atattccaag gcgactgaat ccacagcccg accaccacca
      781 gccacaccag cgtgaccggg atgaagatct tccagcccag ccgcatgatc tggtcgtagc
      841 ggaagcgcgg gaagctcgcc cgcacccaca ggaacacggt gacgacgcag aaggtcttgc
      901 cgaacagcca ggcccagttc cagaaccatg cggtgtgctg gatccattcc ggcgtggtca
      961 tgccgaacag gccgaacgtc accggcgcat accagccgcc gaggaacatg atcaccgcga
     1021 tcgccgagat caggatcatg ttcgcgtatt cggcgaggaa gaacatcgcg aacgccatgc
     1081 ccgagtactc gaccatgtgg ccggcgacga tctcggactc gccttcgacg acgtcgaacg
     1141 ggtgccggtt cgtctcggcc aggcccgaga tgaaatagac gacggcgatc ggcagcagcg
     1201 gcagccagtt ccacgacagg aaggtccagc cgctttcggc cagcatgccg cggccctgct
     1261 ggagcacgat gtcggtcacg ttcaggctgc cgctgaccat cagcaccacg accaggcaga
     1321 agccgatcgc gatctcgtag ctgaccatct gcgccgacgc acgcagcgcg ccgaggaacg
     1381 cgtatttcga gttcgaggcc cagccggcga tgatcacgcc atagacctcg accgaggtga
     1441 tcgccatcag cagcagcagc ccggcgttca ggtccgacag cgcggtctgc ggcccgaacg
     1501 ggatcacgac ccacgcggcc agcgccggca tgatcgtcat caccggcccg aggaagaaca
     1561 gcggcttgtt cgcggcgctc gggacgatga tctccttgaa gatcagcttc accgcgtcgg
     1621 cgatcggctg cagcaggccg agcggaccga cgcggttcgg gcccgggcgg atctggctcc
     1681 agccgatggc cttgcgctcc cacagggtca ggtaggcgac gcagatcatc agcggcagca
     1741 ccagcgcgac gatcttgacg atgctccaca gcaccatcca gctcgccgga tagctctgcg
     1801 gaacggccag cagccagtcg atcatggccc gaccttttcg atggtgatgg cgccgaacat
     1861 cgcgccgagc gcggcggtgt cggggtggcc cgcggggacg cgcaccgcgc tcgccgccag
     1921 cgtcgcgtcg acacgcgccg gcagcaccgc ctgtgcggcg ccctgcgcca cgcgcacctt
     1981 gtcgccggcg ctcaggccga gccgcgacca gagttgcggg ggcaggctcg cgtacggcgc
     2041 gcgcgcgtcg gcggtgagct gcagcgagcc ggcgcggcgc accagcgcat cggtggcata
     2101 gatcggcacg tcggcgatcc gctccagacc gttgcggagg ctgcccacat cgtcacgcgg
     2161 gctcgcgctg ctctggttgc tcagccgcgc ggcgatcgtt gcgaggtcgc cgagcgcttc
     2221 ggcgcgcacc tcgtcggagg tctcgaactc gaagccgtcg agcgccagca ggttgcccag
     2281 cacgcgcagc accttccagg ccgggcgcgt gtcgccgagc ggcttcacga caccatggaa
     2341 actctgcacc cgaccttcgg cattgacgaa gctgcccgag gtctcggtga agggcgcgat
     2401 cggcagcagc acgtcggcga cgtcgtgggc cgcgttcttg aacgggctca tcaccacgac
     2461 catttcggcg gcgtgcatcg ccgcgctcgc ggcggccgcg tcggccgcgt ccagcgtcgg
     2521 ttcgacattc aacagcacgc aggccttcag gtccgggccg ccgagcatct gccccgcagt
     2581 caggccgccg gcacgcggca tcgcgccgac gagttgcgcg ccgaccgagt tggcggcttc
     2641 gctcaggtaa cccacgctcg cgccggtctg cgcgccgatc cagttcgcca gcgcgagcag
     2701 cgcggccgcc cgcggatgct gcgcggctgc gttgccgagc aggatcgcct tgcgctcgcc
     2761 gctcagcagc gacgccgcga tcgccttcgc ctcgtcggtc gcgtcgccgg ccatcggcgc
     2821 ctgcgcgccg ttgccggcag caacggccgt ggcgatgtcc gccagcgcct gcagccagcc
     2881 gctgggcgcg accgagcact gcgaggccat gggcatcagc cagtcgtcgt gcacggcgtt
     2941 caggctgtgc accttcgcgc ccttgcgcgc ggcctggcgg atgcgctgcg cgaacagcgg
     3001 gtggtccttg cgcaggaacg agccgacgac gagcacgcgc tgcagggtcg acagcgcggc
     3061 gatcgacgtg ccgagccagc gcgccgacgg cgccccggcg tcggcattgg cgacattcgt
     3121 gaagtcggcg tggcgcgtgc ggtgatcgat gttctcgctg ccgaggccac gcatgagttt
     3181 cgcgagcagg tgcagttcct cgacgctgct gtgcgccgaa ccgagcgcgc cgacgctctg
     3241 cggcccgtga tcgacgctga tctgcgtcag gccgcggctc acgtattcga gcgcggtcga
     3301 ccagtcgacc gtcttccacg cgccgccctg cttgatcatc ggcgcggtca ggcgctcggg
     3361 gccgttcaac gcctcgtacg agaagcggtc gcggtcggcg atccagcact cgttgatgtc
     3421 ctcgacctcg agcggcacga cacgcatcac ctggttgttc ttgacctgca cgatcaggtt
     3481 ggcccctagg ctgtcgtgcg ggctgatgct cttgcggcgc gacagctccc aggtgcgggc
     3541 gctgtagcgg aacggcttgc tcgtgagcgc gccgaccggg cagacgtcga tcatgttgcc
     3601 ggagagctcg gagtcgatgg tctcgccgac gacggtcgtg atctcggagt gctcgccgcg
     3661 gtggatcatg ccgagctcca tcacgccggc cacttcctgg ccgaagcgca cgcagcgcgt
     3721 gcagtggatg cagcggctca tctcttccat cgagatcagc ggtccgacgt tcttgtggaa
     3781 gacgacgcgc ttttcttcgc ggtaccgcga cgcggagtcg ccgtagccca ccgccaagtc
     3841 ctgcagctgg cactcgccgc cctggtcgca gatcgggcag tcgagcgggt ggttgatcag
     3901 caggaactcc atcacgccct tctgggcctg gagtgccttc gcgctcttgg tgcgcacgat
     3961 catgccctgc gtgaccggcg tggcacaggc cggcatcggc ttgggcgcct tctcgacgtc
     4021 aaccaggcac atgcggcagt tggccgcgat gctgagcttc ttgtgatagc agaagtgcgg
     4081 aatgtaggtg ccggcgttgt cggccgcatg catgatcatg ctgccttcga gcacgtccac
     4141 cttcttgccg tcgagttcga tttcgatcat ttttctcagg ctgcgatcgc cggttcggca
     4201 acgacgacct tcgcggtctt gtgctcgatg aggtggacga actcgtcgcg gaagtgcttg
     4261 atcatcgcgc gcaccggcat cgccgcagcg tcgccgagcg cgcagatcgt gcggccctgg
     4321 atgttgtcgg agaccgagtt caagagctcg atgtcttcca tccgcccctg gccgttggcg
     4381 atgcgctcga ccatgcggaa cagccagccg gtgccctcgc ggcacggcgt gcactggccg
     4441 cagctctcgt gcatgtagaa gtagctcagg cgcagcaggc tcctgaccat gcagcggctg
     4501 tcgtccatca cgatcaccgc acccgagccg agcatcgagc cggccttggc gatgctgtcg
     4561 tagtccatcg tcagctcgtt catgatcgcc gcgggcagca ccggcgccga cgagccgccg
     4621 gggatcacgg ccttcagcgt gcggccgggg cgcacgccgc cggcgagttc gagcagcttg
     4681 ctgaaaggcg tgccgagcgg gatctcgtag ttgcccggct tctgcacgtc gccgaccacc
     4741 gagaagatct tcgtgccgcc gttgttcggc ttgccgatcg ccaggtacgg tgcgccgccg
     4801 ttgcggatga tccagggcac tgccgcgaag gtctcggtgt tgttgatcgt cgtgggcttg
     4861 ccgtacaggc cgtagctggc cgggaacggc ggcttgaagc gcggctggcc cttcttgcct
     4921 tcgagcgatt cgagcagcgc ggtttcttcg ccgcagatgt aggcgccgaa gccgtgcgcc
     4981 gcatgcagct ggaagctgaa gctcgagccg agcaggttgt cgccgaggta gccggcggcg
     5041 cgcgcttcct cgagcgcttc ctcgaagcgg tcatagacct cgaagatctc gccgtggatg
     5101 tagttgtagc cgaccttgat gcccatcgca tacgccgcga tcgccatgcc ttcgatgacg
     5161 atgtgcgggt tgaacatcag gatgtcgcgg tccttgcagg tgccgggctc gccttcgtcg
     5221 gaattgcaga cgaggtactt cgcgcccggg aactggcgcg gcatgaagct ccacttcagc
     5281 ccggtcggga agcccgcgcc gccgcggccg cgcaggcccg agtccttcac cagctggatc
     5341 aggtcgtcgg ggtgcacgtc cagggccttc ttgagggccg cgtagccgtc cagccgttcg
//
LOCUS       DATMJB010000570         3822 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_674592, whole genome shotgun sequence.
ACCESSION   DATMJB010000570 DATMJB010000000
VERSION     DATMJB010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674592"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..187)
                     /locus_tag="VNU48_12165"
     CDS             complement(17..187)
                     /locus_tag="VNU48_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12077.1"
                     /translation="MVYRIVAERDNETVRMERASSLLALAKAAVWASEGWEVTIIDCE
                     EDPAQSHELAAA"
     gene            complement(305..2626)
                     /locus_tag="VNU48_12170"
     CDS             complement(305..2626)
                     /locus_tag="VNU48_12170"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWI12078.1"
                     /translation="MTGPLLGRSINRLEDPRFVRGRGCYVDDLAAPNALHGVVVRSKH
                     AHARIVAIDVDAARRMPGVAAVFTGSDLAADDIGPLPCAVRQIPMTRPLVVPPCHALA
                     RDVVRYVGEPVAFVVAESAGRARDAAEAVVVDYEPLPPIVSLADAVLPGAPSIWPEAI
                     GNIAFQFNSGEIGPVEAAIGNAAHVVECELVNNRVVAAPLETRGALGAFDASNGRLHL
                     TASAAGAHGIRNLLAASVFRIPREQLRVSIPDVGGGFGMKNVLYPEWVLVLWAARRLG
                     RQVKWIGDRSEDFVASAHGRDSLVRARLALDRDGRFLALETKVLANLGGYVSTVAPVV
                     PTTAMASAMGGVYDIPLIAFQASGVFTNTTPVDAYRGAGKPEANYLIERCIDIAAAEL
                     GMDALKLRRKNIFRRFPHRSAMGLSVEQGSFAHGIDHAVAAAADFVARRKNSRARGRL
                     RGLGYACFLETSRGQPNEVAEVRIGEDGGIDLLVGTHSNGQGHETTYAQIAADTLGLP
                     LGRFRFRQGDTDDLDSGGGHGGARSMHQGGTALLLAAEGLIENARRLAARLLQTALDG
                     VDYEAGLLRVAATGQEISLDEVARVSYQAPGDDVAPGLAYKATHLCDRYTFPNGCHVA
                     EVEIDPETGAVTLDRYVIFDDYGSLLDPRQTLGQVHGGVVQGIGQALFEHALFDTETG
                     QILSGSLMDYALPRADDIPSFEGNLTSDFPSGANRLGVKGSGQAGAIGAPATIMNAVM
                     NALAPLGVRHLDMPATPSRIWHAIRSAKLPVGS"
     gene            complement(2777..>3822)
                     /locus_tag="VNU48_12175"
     CDS             complement(2777..>3822)
                     /locus_tag="VNU48_12175"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWI12079.1"
                     /translation="INIPDTVGYTIPEEYFAMFKMVRERVPNSDKAVFSAHCHNDLGM
                     AVANSMAAAQAGVRQIECTVNGIGERAGNAALEEVVMAINTRNDKFPWWNQIDTTMLT
                     RASKLVSAATSFPVQYNKAIVGRNAFAHESGIHQDGVLKDASTYEIMRPEMVGLKKSS
                     LVLGKHSGRHAFVHKLEEMGYKLGANQLEDAFARMKALADRKKDIYDEDIEALVDQEI
                     AASHDRIKLASLTVIAGTHGPQRATMKLDVDGVIKIEEAEGNGPVDAVFNCIKALVPH
                     EAKLELYQVHAVTEGTDAQAEVSVRLAHEGRSMTSKASDPDTLVASAKAYLGALNKIV
                     MKRQRDMPAQAAG"
BASE COUNT          662 a         1270 c         1215 g          675 t
ORIGIN      
        1 gcccaccgga acctgactat gctgcagcca gctcatgcga ttgagccggg tcttcttcac
       61 aatcgataat cgtgacctcc cagccctcac ttgcccatac cgccgccttc gccagcgcca
      121 gcagcgagct ggcccgctcc atccgcaccg tctcgttgtc ccgctcggcc acaatccgat
      181 aaaccatcac agtcccctta tgcatatcca ttggcaacga agggacattc gattcccaat
      241 ttgactgcaa ttcgccggca aagtggccgg ctggtggcgt ggtcagggcg caattgcttc
      301 ggcgtcagga cccgactggc agcttggcgg accggatcgc gtgccagatt cgggagggcg
      361 tcgcgggcat gtcgagatgc cggacgccca gcggcgccag cgcgttcatc accgcgttca
      421 tgatggtggc cggggctccg atcgcgccgg cctgaccgct tcccttcacg cccagccggt
      481 tggcgccgct tggaaaatcg gacgtcagat tgccctcgaa cgacggaatg tcgtccgcgc
      541 gcggcagcgc ataatccatc agcgagcccg agaggatctg gcccgtctcc gtgtcaaaca
      601 gcgcgtgctc gaacagcgcc tggccgatgc cctgcaccac gccgccatgg acctgcccca
      661 gggtctggcg cggatcgagc aggctgccgt agtcgtcgaa gatgacgtag cggtcgagtg
      721 tcaccgcacc ggtctcaggg tcgatttcga cttcagcgac atggcacccg ttgggaaagg
      781 tgtagcggtc gcaaaggtgg gtcgccttgt acgccaggcc gggggcgaca tcgtcgccgg
      841 gcgcctggta tgagacacgc gccacctcat cgaggctgat ctcctgcccg gtcgcggcca
      901 cgcgcaacag gcctgcttca taatcgacgc cgtcgagagc ggtctgcagc aaccgcgcgg
      961 caagccgccg cgcattctcg atcaggcctt cggccgcgag cagcagcgcg gtgccaccct
     1021 ggtgcatcga gcgcgcgccg ccatggccgc cgccgctgtc gagatcgtcg gtatcgccct
     1081 gccgaaaccg aaaacgccca agcggcaagc caagcgtatc ggccgcgatc tgggcatagg
     1141 tggtttcatg gccctgaccg ttggaatggg tgccgactaa cagatcgatc ccgccgtcct
     1201 cgccgatgcg cacttccgcc acttcattgg gctggccgcg cgaggtttcg agaaagcagg
     1261 cgtagccgag gccgcgcagc ctgccacgcg cgcgagagtt cttccggcgt gcaacgaagt
     1321 ccgccgccgc ggcgaccgcg tgatcgatcc cgtgagcaaa gctgccctgc tcgaccgaaa
     1381 gccccatcgc gctgcgatgg gggaaacggc gaaagatgtt cttgcgccgc agctttagcg
     1441 cgtccatccc gagttcggca gcagcaatgt cgatgcagcg ttcgatcaga tagttcgcct
     1501 cgggcttgcc ggcgccgcga taggcatcga ccggcgtggt gttggtgaac acgccgctgg
     1561 cctgaaatgc gatcagcggg atgtcgtaga cgccgcccat cgcgctcgcc atcgccgtcg
     1621 tcggcaccac gggcgctacc gtcgagacat agccgcccag attggcgagg actttggttt
     1681 cgagcgccag gaagcggcca tcccggtcca gcgcgagccg agcccgaacg aggctgtcgc
     1741 ggccatgcgc ggaggccacg aaatcctcgc tgcggtcgcc gatccacttg acctggcggc
     1801 cgagccggcg cgccgcccac agcaccagca cccattccgg atagagaacg tttttcatcc
     1861 caaaaccgcc gccgacgtcg ggaatgctga cgcggagttg ttcacgggga atgcggaaaa
     1921 cggaggcggc gagcaggttg cggatgccgt gcgccccggc cgccgaggcg gtcaggtgca
     1981 ggcggccgtt tgaggcatcg aacgcgccga gcgcaccgcg ggtttccagc ggggcggcga
     2041 caacgcggtt gttgaccaat tcgcattcca ccacatgggc ggcgttgccg atcgccgctt
     2101 ccaccggccc gatctcgccg ctgttgaact ggaacgcgat gttgccgatg gcctccggcc
     2161 agatcgacgg cgctcccggc aaaacggcat ctgccagtga gacgatcggc ggcagcggct
     2221 cgtagtcgac gacgacggct tcggcggcat cgcgggctct tccggcgctt tcggcgacca
     2281 cgaacgcgac cggctcaccg acatagcgaa cgacatcgcg cgccagtgcg tggcagggcg
     2341 ggaccacgag tggcctggtc atcggaatct gcctcaccgc gcaaggcagc gggccgatat
     2401 cgtcggcagc cagatccgat ccggtgaata ccgccgcaac gccgggcatc cggcgcgccg
     2461 catcgacgtc gatcgcgacg atgcgtgcgt gcgcatgctt cgagcgaacc accacaccat
     2521 gaagcgcatt tggcgccgcg agatcgtcga cgtagcaccc gcgcccccgc acgaagcgcg
     2581 ggtcttcaag acggttgatg ctgcgtccca ataacggccc ggtcatgtga aaatctatgg
     2641 aaccagacgg ctgcaaaggc aacagctggc cgcggcggtg gccgacgcaa taaaaacgcg
     2701 gcgctgaggg cgccgcgttt gggttggatt gtcacgtcat tccgggcgca tcgaccatcc
     2761 cggaatgaca attcggtcag cccgccgctt gcgccggcat gtcgcgctgg cgcttcatga
     2821 cgatcttgtt gagcgcgccg agataggctt ttgcagacgc caccagcgta tccggatcgg
     2881 acgccttcga agtcatcgag cggccctcat gggccaaccg taccgacact tcagcctgcg
     2941 cgtcggtgcc ctcggtgacg gcgtggacct gatagagctc cagcttggcc tcgtgcggca
     3001 ccagcgcctt gatgcagttg aacaccgcat cgaccggacc gttgccttcg gcttcctcga
     3061 tcttgatcac gccgtcgacg tcgagcttca tggtggcgcg ctgcggccca tgggtgcccg
     3121 cgatcacggt cagcgacgcc agcttgatgc gatcgtggga ggcggcgatt tcctggtcga
     3181 ccagcgcctc gatgtcctcg tcgtagatgt ccttcttgcg gtcggccagc gccttcatcc
     3241 gcgcgaacgc gtcttccagc tggttggcgc cgagcttgta gcccatctcc tccagcttgt
     3301 gcacgaaggc atggcggccg gaatgcttgc cgagcaccag cgaagacttc ttcaggccga
     3361 ccatttcagg gcgcataatc tcgtaggtcg atgcgtcctt cagcacgccg tcctggtgga
     3421 tgccgctttc atgcgcgaac gcgttgcggc cgacgatggc cttgttgtac tgcaccggga
     3481 acgaggtcgc cgccgagacc agtttcgatg cccgggtcag catggtggtg tcgatctggt
     3541 tccaccacgg aaacttgtcg ttacgggtgt tgatcgccat cacgacttcc tcgagcgcgg
     3601 cattgcctgc ccgctcgcca atgccgttga ccgtgcattc gatctgacgc acgccggcct
     3661 gtgccgccgc catggaattg gcgaccgcca tgccgagatc gttatggcaa tgtgcggaga
     3721 acaccgcctt gtccgaattc ggcacgcgct cgcgtaccat tttgaacatg gcgaaatatt
     3781 cttccgggat cgtgtagccg accgtgtcgg ggatattgat cg
//
LOCUS       DATMJB010000571         2040 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_616145, whole genome shotgun sequence.
ACCESSION   DATMJB010000571 DATMJB010000000
VERSION     DATMJB010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616145"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /gene="trpB"
                     /locus_tag="VNU48_12180"
     CDS             <1..995
                     /gene="trpB"
                     /locus_tag="VNU48_12180"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWI12080.1"
                     /translation="NHTGAHKVNNTIGQALLARRMGKPRVIAETGAGQHGVATATICA
                     RYGLECVVYMGSEDVKRQSPNVYRMNLLGATVVPVESGSKTLKDALNEALRDWVTNVE
                     NTFYIIGTVAGPHPYPTMVRDFQRVIGDECLVQMPEMTGRQPDAVIACVGGGSNAMGI
                     FYPYIDHAGTRLIGVEAAGQGLDTGKHAASLSAGSPGVLHGNRTYLLQDANGQIIETH
                     SISAGLDYPGVGPEHAYLKDIGRAEYVGITDREALAAFHRLCRTEGIIPALESAHAVA
                     HAMKIAPTMRVDQHLLVNLSGRGDKDIGTVADLSGAAYFDRPSQAGQSVKGRGA"
     gene            992..1789
                     /gene="trpA"
                     /locus_tag="VNU48_12185"
     CDS             992..1789
                     /gene="trpA"
                     /locus_tag="VNU48_12185"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWI12081.1"
                     /translation="MSRIKATFDALQAQGRKALIPYVTSGDPFADATVDIMLAMAAAG
                     ADVIELGVPFSDPMADGPVIQKAGERALARGIGMPQVLAFVRGFRQHNDTTPVVLMGY
                     ANPIERYGIDRFVADAKAAGVDGVLVVDYPPEECEGFAATLRANAIDPIFLLAPTSTE
                     TRMAQVGRIASGYVYYVSLKGVTGAGHLDTAAVATMIPRIKRHVKLPVGVGFGIRDAK
                     TAQAVAAVSDAVVIGTALVQLLESCTRDNVAAAAGEFIAGIRAALDS"
     gene            1804..>2040
                     /locus_tag="VNU48_12190"
     CDS             1804..>2040
                     /locus_tag="VNU48_12190"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484517.1"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer of AccA2D2 subunits; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HWI12082.1"
                     /translation="MSWLEKLLPPKIQPNERGERRTVPEGLWIKCPQCETVLYKTDLE
                     QNINVCPTCSHHHRIGARARLDAFLDPEGRFEIGQ"
BASE COUNT          353 a          692 c          696 g          299 t
ORIGIN      
        1 tgaaccacac cggtgcgcac aaagtcaaca acacgatcgg acaggcgttg ctcgcgcggc
       61 gcatgggcaa gccgcgcgtg attgccgaga ccggcgccgg ccagcacggc gtcgcgaccg
      121 cgacgatctg cgcgcgctac ggcctggagt gcgtggtcta catgggcagc gaggatgtga
      181 agcgccaatc gccgaacgtc taccgcatga acctgctcgg cgcgaccgtg gtgccggtcg
      241 agagcggctc gaagacgctg aaggatgcgc tgaacgaggc gctgcgcgat tgggtcacga
      301 acgtcgagaa caccttctac atcatcggca cggtcgccgg cccgcacccg tacccgacga
      361 tggtgcgcga cttccagcgc gtcatcggcg acgagtgcct ggtgcagatg cccgagatga
      421 ccggccgcca gccggatgcg gtgatcgcct gcgtgggcgg tggcagcaac gcgatgggca
      481 tcttctaccc gtacatcgac catgccggca cgcgcctgat cggcgtcgag gcggcggggc
      541 aggggctgga caccggcaag cacgccgcgt cgctcagcgc cggctcgccg ggcgtgctgc
      601 acggcaaccg cacctatctg ctgcaggatg cgaacggcca gatcatcgag acgcattcga
      661 tctcggcggg tctggactat cccggcgtcg ggcccgagca cgcgtacctg aaggacatcg
      721 gccgcgccga gtacgtgggc atcaccgacc gcgaagcgct cgccgcgttc catcgcctgt
      781 gccgcaccga aggcatcatc ccggcgctcg agtcggccca cgcggtggcg cacgcgatga
      841 agatcgcacc gacgatgcgt gtcgatcagc acctgctcgt gaatctctca ggccgcggcg
      901 acaaggacat cggcacggtt gccgacctgt ccggtgcggc gtacttcgat cgcccctcgc
      961 aagccggcca aagcgtaaag gggcgcggcg catgagccgc atcaaggcca ccttcgacgc
     1021 gctgcaggcg caaggccgca aggcgctgat tccgtacgtg acctcgggcg atccgttcgc
     1081 cgacgcgacc gtcgacatca tgctggcgat ggctgcggcc ggtgccgacg tgatcgaact
     1141 cggcgtgccg ttctccgacc cgatggcgga cggcccggtg atccagaagg ccggcgagcg
     1201 tgcgctggcg cgcggcatcg gcatgccgca ggtgctggcc ttcgtgcgcg gcttccgcca
     1261 gcacaacgac accacgccgg tcgtgctgat gggctacgcg aacccgatcg aacgctacgg
     1321 catcgatcgc ttcgtcgccg acgcgaaagc ggccggcgtc gacggcgtgc tcgtcgtcga
     1381 ctacccgccc gaggaatgcg agggcttcgc ggccacgctg cgggcgaacg cgatcgatcc
     1441 gatcttcctg ctcgcgccga cctcgaccga gacgcgcatg gcgcaggtcg gccggatcgc
     1501 cagcggctac gtctactacg tgtcgctgaa gggtgtgacg ggcgccggcc acctcgacac
     1561 ggcggccgtc gcgacgatga tcccgcgcat caagcggcac gtgaagctgc cggtgggcgt
     1621 gggctttggc atccgcgacg cgaagacggc gcaggcggtc gcggcggttt ccgacgcggt
     1681 cgtcatcggc acggccttgg ttcaattgct ggaaagctgc acacgcgaca atgtggccgc
     1741 tgcggcgggc gagttcatcg ccggcatccg cgcggcattg gattcctgaa aggatcacgt
     1801 tccatgagct ggctcgaaaa actgctgcct ccgaagattc aaccgaacga acgcggcgag
     1861 cgccgcaccg tgcccgaagg gctgtggatc aagtgcccgc agtgcgaaac ggtgctgtac
     1921 aagaccgacc tcgagcagaa catcaacgtc tgcccgacgt gcagccacca ccaccgcatc
     1981 ggcgcgcgcg cgcgcctcga tgcgttcctc gaccccgagg gtcgtttcga gatcggccag
//
LOCUS       DATMJB010000572         2420 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_587911, whole genome shotgun sequence.
ACCESSION   DATMJB010000572 DATMJB010000000
VERSION     DATMJB010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587911"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..775
                     /locus_tag="VNU48_12195"
     CDS             17..775
                     /locus_tag="VNU48_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HWI12083.1"
                     /translation="MPQQRLAPGGVARLALGASTEAPRASYHGVPVLVLRDRGQWTAV
                     LGIALAATPGRAVLTLQRGDAAPTELPFTIKPFHYAEQRLSVAPGQVDLSPQDLARYE
                     RERAHLAEVVATFGSAVPETLRLRQPVPGARSSSFGLRRVFNGQSRNPHSGMDIAAPL
                     GTPVVAAAAGTVIDTGDYFFNGNTVWLDHGAGLLTMYCHLSAISVAVGDTVAAGAPLG
                     AVGATGRATGPHLHWSVSLNRAMVDPALFLAPAH"
     gene            complement(777..>2420)
                     /locus_tag="VNU48_12200"
     CDS             complement(777..>2420)
                     /locus_tag="VNU48_12200"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWI12084.1"
                     /translation="VRNPERARDRRNLVLAQMVKHGTLPAARYEQLRALPLVLAFKRP
                     EDGDIGQARHFVAHLREQLVDWADAHDVDLDTDGLVIRTTLDAKLQRLAQAAVTQQVA
                     LLQRIAGEEWSEARPRAARVQQPMAPAKGAAAIEPFAWFWRQHAELLDEIARDTPQFR
                     AARQAGQGDAQALATVLADSATMARLRDERTRLSAGFIAIDPATGAVRAWVGSADFTR
                     EQFDHVAQARRQPGSTFKPFVYGAALQRGLSTEHQYLDGAVDIALADGSSWQPTDGSG
                     PSGEMMTLREGLVQSKNTITAQVMQDVGAEAVVNFAQAAGVRDSTLAPVPSLALGTSP
                     VTLLEMGNAYATLAALGERHPPLLVTQIADRNGRVLETWAPQAERVLDPALIAKLVDV
                     MRGVTQTGTGAAIRAEWGVKGDLAGKTGTTQNNTDGWFMLMTPKLVTGAWVGFNDPRV
                     TIRGNYWGQGGHNALRLVGAFMQQAQRSKLIDAKAEFPQVERDPPEAPALPASAADPD
                     FGPAGPSPVSEVTRVLGLPTVTPSEAAAAAQAREERMGR"
BASE COUNT          273 a          906 c          894 g          347 t
ORIGIN      
        1 ggcgcacgcg accccgctgc cgcagcagcg cctcgcgccg ggcggcgtcg cacgcctcgc
       61 gctcggcgcg tcgaccgagg cgccgcgcgc cagctaccac ggcgtgccgg tgctggtgct
      121 gcgcgatcgc ggccagtgga ccgcggtgct cggcatcgcg ctggccgcga cgccgggccg
      181 cgcggtgctg acgctgcagc gcggcgacgc cgcgccgacc gagctgccct tcacgatcaa
      241 gccgttccac tacgccgagc agcgtctgag tgtcgcgccc ggccaggtcg acctgtcgcc
      301 gcaggacctg gcgcgctacg agcgcgagcg tgcgcacctg gccgaggtcg tcgcgacctt
      361 cggcagcgcc gtgcccgaga cgctgcggct gcgccagccg gtgcccggcg cgcgctcgag
      421 ttcgttcggg ttgcggcgcg tgttcaacgg ccagtcgcgc aatccgcaca gcggcatgga
      481 catcgccgcc ccgctgggca cgccggtggt cgcggcggcc gcgggcacgg tgatcgacac
      541 cggcgactac ttcttcaacg gcaacaccgt ctggctcgac cacggcgcgg ggctgctgac
      601 gatgtactgc cacctgtcgg cgatctcggt cgcggtcggc gacacggttg ccgcgggcgc
      661 gccgctcggc gcggtcggtg cgaccggccg cgccaccggg ccgcacctgc actggtcggt
      721 gagcctgaac cgcgcgatgg tcgacccggc gctgttcctc gcgccggcgc actgattcag
      781 cggcccatcc gctcctcgcg cgcctgcgcc gccgccgccg cttcggaggg cgtcacggtc
      841 ggcaggccaa gcacgcgggt cacctcggag acgggcgacg gcccggccgg gccgaagtcc
      901 ggatcggccg ccgaggcggg cagtgccggc gcctccggcg gatcgcgttc gacctgcgga
      961 aactcggcct tcgcgtcgat cagcttgctg cgctgggcct gctgcatgaa cgcgccgacg
     1021 aggcgcagcg cgttgtgccc gccctgcccc cagtagttgc cgcggatcgt cacgcgcggg
     1081 tcgttgaacc cgacccatgc gccggtgacg agcttgggcg tcatcagcat gaaccagccg
     1141 tcggtgttgt tctgggtcgt gccggtcttg ccggcgaggt cgcccttcac gccccactcg
     1201 gcgcggatcg ccgcgccggt gccggtctgc gtgacgccgc gcatcacgtc gacgagtttc
     1261 gcgatcagcg ccgggtcgag cacgcgctcg gcctgcggcg cccaggtctc gagcacgcgg
     1321 ccgttgcgat ccgcgatctg cgtgaccagc aacggcgggt gccgttcgcc gagcgcggcc
     1381 agcgtcgcgt aggcgttgcc catctcgagc agcgtgacgg ggctggtgcc gagcgcgagc
     1441 gagggcaccg gggccagcgt cgagtcgcgc acgccggcgg cctgcgcgaa gttgacgacc
     1501 gcctcggcgc cgacgtcctg catcacctgc gcggtgatcg tgttcttcga ctgcaccagg
     1561 ccttcgcgca gcgtcatcat ctcgccgctc ggcccgctgc cgtcggtcgg ctgccagctg
     1621 ctgccgtcgg ccagcgcgat gtcgaccgcg ccgtccaggt actggtgttc ggtggacagg
     1681 ccgcgctgca gcgccgcgcc gtagacgaag ggcttgaagg tcgagcccgg ctggcgcctg
     1741 gcctgcgcga catggtcgaa ctgctcgcgc gtgaaatcgg cgctgccgac ccaggcccgc
     1801 accgcgccgg tggccgggtc gatcgcgatg aagccggcgg acagccgggt gcgctcgtca
     1861 cgcagccgcg ccatcgtcgc gctgtcggcg agcaccgtcg cgagcgcctg tgcgtcgccc
     1921 tggccggcct gccgcgcggc gcggaactgc ggcgtgtcgc gcgcgatctc gtcgagcagc
     1981 tccgcgtgct ggcgccagaa ccacgcgaac ggttcgatcg ccgccgcgcc cttggccggc
     2041 gccatcggct gctgcacgcg tgcggcgcgc ggccgcgcct cgctccattc ctcgccggcg
     2101 atgcgctgca gcagcgcgac ctgctgcgtg accgcggcct gcgcgaggcg ctgcagcttg
     2161 gcgtcgagcg tggtgcggat caccaggccg tcggtgtcga gatcgacgtc gtgcgcgtcg
     2221 gcccagtcga ccagctgctc gcgcaggtgc gcgacgaagt ggcgcgcctg gccgatgtcg
     2281 ccgtcctcgg gccgcttgaa cgcgagcacc agcggcagcg cacgcagctg ttcgtagcgc
     2341 gccgccggga gcgtgccgtg cttgaccatc tgcgccagca cgaggttgcg ccggtcgcgc
     2401 gcgcgctccg ggttgcgcac
//
LOCUS       DATMJB010000573         2148 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_16903, whole genome shotgun sequence.
ACCESSION   DATMJB010000573 DATMJB010000000
VERSION     DATMJB010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16903"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VNU48_12205"
     CDS             complement(<1..1031)
                     /locus_tag="VNU48_12205"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HWI12085.1"
                     /translation="MPDTRTGSRDRAVPFTSVNFDDAFWAPRQRAVRETTIPFLYGQC
                     EKAGMIDALDVRSPPGPLPIAFQQTPIGTKPATPVMYWDSDLGKWIEAASYALATRRD
                     AELEALIDDVVARIEAAQEPDGYFNTYFQRREPGKKWTNLRDWHELYCAGHLIEGAVA
                     YAAATGKTQLLAVIKRYVALIDRLFGPRAGQKRGYCGHEEIELALVKLHRFTGEQLPL
                     DLARYFVDERGRRPHYFDVEARARGEDPAAWYHRTYDYNQSELPVREQAKVTGHAVRA
                     MYLYSAMADLAAEDGDRGLHEACTRLWNDLTRKRLYVTGGLGPSWTNEGFTRDYDLPN
                     DTAYAETCAA"
     gene            complement(1051..1893)
                     /locus_tag="VNU48_12210"
     CDS             complement(1051..1893)
                     /locus_tag="VNU48_12210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWI12086.1"
                     /translation="MSVALRLPGAARGWHLTALAMLGVALYLFPIYWMFASGLKNSAE
                     IFASPPTLIPRAPTLAAYEYAFVRENVLRYLRNSLAIAVPVAALTLVLGAMGGYALSR
                     LRTRLVDAALFVVLLLQVFPEALLATPMFILFKALGLLNTFAAVILATTSKTLAFALI
                     VLRPLFRQVPAELEEASLVDGCTPLQAFARIVVPLMAIPLMVVGALSFVQAYGQFVYA
                     LTFLTRQELQPATVGIYSFVGGEYADWHRVMAFSSVFVLPILALFVLLQRRIVAGLTA
                     GALK"
     gene            complement(1890..>2148)
                     /locus_tag="VNU48_12215"
     CDS             complement(1890..>2148)
                     /locus_tag="VNU48_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWI12087.1"
                     /translation="PALRPALLAVACLVTIYTMRAFDLIFAMTQGGPLDSSNVLPLLS
                     YQLSFQQFQFGAGSAVGTFAFVIVFAVALLYVRTRAKETPE"
BASE COUNT          353 a          708 c          794 g          293 t
ORIGIN      
        1 gccgcgcagg tctcggcgta ggcggtgtcg ttgggcaggt cgtagtcgcg cgtgaagccc
       61 tcgttggtcc aggacgggcc gaggccgccg gtgacgtaga gccgcttgcg cgtgaggtcg
      121 ttccacagcc gcgtgcaggc ctcgtgcagg ccgcgatcgc cgtcctcggc ggcgaggtcg
      181 gccatcgccg agtagagata catcgcgcgc accgcgtggc cggtgacctt ggcctgctcg
      241 cgaaccggga gctccgactg gttgtagtcg taggtgcggt ggtaccacgc ggcgggatcc
      301 tcgccgcgcg cgcgcgcctc gacgtcgaaa tagtgcggtc ggcggccgcg ctcatcgacg
      361 aagtagcgcg cgagatcgag tggcagctgc tcgccggtga agcggtggag cttgaccagc
      421 gcgagctcga tctcctcgtg cccgcaatag ccccgcttct gcccggcgcg cgggccgaac
      481 agccggtcga tcagcgcgac gtagcgctta atcacggcga gcagctgcgt cttcccggtc
      541 gccgcggcat aggcgaccgc gccctcgatc aggtggccgg cgcagtagag ctcgtgccag
      601 tcgcgcaggt tggtccactt cttgccgggt tcgcggcgct ggaagtaggt gttgaagtag
      661 ccgtcgggct cctgcgccgc ctcgatccgc gcgaccacgt cgtcgatcaa cgcctcgagc
      721 tcggcgtcgc gccgcgtcgc cagcgcgtag gaggcggcct cgatccactt gccgaggtcg
      781 gagtcccagt acatcaccgg cgtcgccggc ttggtgccga tcggcgtctg ctggaaggcg
      841 atcggcagcg ggccgggcgg cgagcgcacg tcgagcgcgt cgatcattcc cgccttctcg
      901 cactggccgt agaggaacgg gatcgtcgtc tcgcgcaccg cgcgctggcg cggcgcccag
      961 aaggcgtcgt cgaagttcac cgaggtgaac ggcaccgcgc ggtcgcgcga accggtccgc
     1021 gtgtcaggca tcgtggtcac tccgttgcgg tcacttgagc gcccccgcgg tcagcccggc
     1081 gacgatgcgc cgctgcagca ggacgaaaag cgcgaggatc ggcagcacga acaccgacga
     1141 gaacgccatc acgcggtgcc agtcggcgta ttcgccgccg acgaagctgt agatgccgac
     1201 ggtggcgggt tggagctcct gccgggtgag gaaggtgagc gcgtagacga actggccgta
     1261 ggcctggacg aagctgagcg cgccgacgac catcagcggg atcgccatca gcggcaccac
     1321 gatccgcgcg aaggcctgaa gcggcgtgca gccgtcgacc agcgaggcct cctcgagctc
     1381 ggcgggcacc tggcggaaga gcgggcgcag cacgatcagc gcgaaggcca gcgtcttcga
     1441 ggtggtggcg aggatcaccg cggcgaaggt gttgagcagg ccgagcgcct tgaacaggat
     1501 gaacatcggc gtcgccagca acgcctcggg gaagacctgc agcagcagga cgacgaacag
     1561 cgcggcgtcg accagccgcg tgcgcagccg gctcagcgcg tagccgccca tcgctcccag
     1621 caccagagtc agcgccgcca ccggcacggc gatcgccagg ctgttgcgca ggtagcgcag
     1681 cacgttctcg cgcacgaagg cgtattcgta cgccgccagc gtgggcgcgc gcgggatcag
     1741 cgtcggtggg ctggcgaaga tctcggccga gttcttcagc cccgacgcga acatccagta
     1801 gatcgggaac aggtagagcg cgacgccgag catcgccagc gcggtcaggt gccagccgcg
     1861 cgccgcgccg ggaaggcgga gggcgacgct cactcgggcg tctccttcgc gcgcgtgcgc
     1921 acgtagagca gcgcgaccgc gaagacgatg acgaaggcga aggtgccgac cgcggaaccg
     1981 gcgccgaact ggaactgctg gaacgagagc tggtacgaca gcagcggcag cacgttgctc
     2041 gaatccagcg ggccgccctg cgtcatcgcg aagatcaggt cgaaggcgcg catcgtgtag
     2101 atcgtgacga ggcaggcgac cgcgagcagc gccgggcgca gcgcgggc
//
LOCUS       DATMJB010000574         2642 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_142933, whole genome shotgun sequence.
ACCESSION   DATMJB010000574 DATMJB010000000
VERSION     DATMJB010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142933"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /gene="glyS"
                     /locus_tag="VNU48_12220"
     CDS             <1..1060
                     /gene="glyS"
                     /locus_tag="VNU48_12220"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HWI12088.1"
                     /translation="TLASRVPGLAKVVYHNKLGTQGERVERVRTIARAIAEPLGGEML
                     AREADQAALLAKADLLTDMVGEFPELQGTMGGYYARHDGETEAVAIAIEDHYKPRFAG
                     DALPRNEVGLAVALADKLETLVGLFGIGQLPTGDKDPFALRRHALGVIRMLVERDLPL
                     RFDELVGIAADAMGPLLTEPRAGAQLTDFIYERLAGSLREQGYSAQEVDAVLSLRPPR
                     LADVAARLAAVRAFAALPEAAALAAANKRVGNILKKVDGAVDARVDKGLLKEPAEATL
                     NDALALATPRADAAFERGDYAGSLHALAALRTPVDAFFDSVMVNADDIALRANRLGLL
                     ATLHAAMNRVADLSKLAS"
     gene            1074..1682
                     /gene="gmhB"
                     /locus_tag="VNU48_12225"
     CDS             1074..1682
                     /gene="gmhB"
                     /locus_tag="VNU48_12225"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HWI12089.1"
                     /translation="MKLIILDRDGTINEDRDDFVKTPDEWVPIPGALEAIARLNHAGW
                     HTVVATNQSGLGRGTFDMATLNAMHTKMNQLLAKQGGRIDAVFFCPHAPEEPCHCRKP
                     LPGLFEQIGERFGVALHNVPVVGDSLRDLQAGVAVGCQPHLVRTGKGARMSPAQLDAL
                     CAQVPGTRVHADLTAFAEYIVREERKGRTDTGESDSGFGRLD"
     gene            1682..2437
                     /locus_tag="VNU48_12230"
     CDS             1682..2437
                     /locus_tag="VNU48_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWI12090.1"
                     /translation="MAHFFNAVRSALFVLFMAVTVIPWALAVLLVSIVAKGNVVYWMC
                     VGWLRTAIWGAKVICGVHARLHGFENLPDSPVVLLPKHQSTWETFAFPTLMPHPLCYV
                     FKRELLYIPFFGWAMARMDMIHIDRSKRAEAWNLVAAQGRKFMAKGHWVIMFPEGTRI
                     ARGAKGHYKGGGTRLAIQTERPVLPVAVTSARCWPRKSFVLRPGVIDVSVGKPIPSTG
                     REPDELMREVEAWIETEMRRLDPQAYATPAAPA"
     gene            2449..>2642
                     /locus_tag="VNU48_12235"
     CDS             2449..>2642
                     /locus_tag="VNU48_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12091.1"
                     /translation="MSRRAAALDTQQLSLFEESAPPVAPLAPMQTIEPAAPGVDRAPA
                     APPAEAVASAGGLAPAVFRHP"
BASE COUNT          380 a          943 c          953 g          366 t
ORIGIN      
        1 gacgctcgcg tcgcgggtgc cgggcctggc caaggtcgtc taccacaaca agctcggcac
       61 gcagggcgag cgggtggagc gcgtgcgcac gatcgcgcgc gcgatcgccg agccgctcgg
      121 cggcgagatg ctcgcgcgcg aggccgacca ggcggcgctg ctggcgaagg ccgacctgct
      181 gaccgacatg gtcggcgagt tccccgagct gcagggcacg atgggcggct actacgcgcg
      241 ccacgacggc gagaccgagg ccgtcgcgat cgcgatcgag gaccactaca agccgcgctt
      301 cgccggcgac gcgctgccgc gcaacgaggt cggcctcgcg gtcgcgctgg ccgacaagct
      361 cgagacgctg gtcggcctgt tcggcatcgg ccagctgccc accggcgaca aggatccgtt
      421 cgcgctgcgc cgccatgcgc tcggtgtgat ccggatgctg gtcgagcgcg atctgccgtt
      481 gcggttcgac gaactcgtcg gcatcgccgc cgacgcgatg ggcccgctgc tgaccgagcc
      541 gcgcgcgggc gcgcagctga ccgacttcat ctacgagcgc ctcgccggca gcctgcgcga
      601 gcagggctac agcgcgcagg aggtcgacgc ggtgctgtcg ctgcggccgc cgcggctcgc
      661 cgatgtggcg gcgcgcctgg cggcggtgcg tgcgttcgcg gcgctgcccg aggcggcggc
      721 gctggccgcg gcgaacaagc gcgtgggcaa catcctgaag aaggtcgacg gtgcagtgga
      781 cgcacgcgtc gacaagggcc tgttgaagga acccgcggaa gccacgttga acgacgcgct
      841 cgcgctcgcg acgccgcgcg ccgacgccgc gttcgagcgc ggcgactacg ccggttcgct
      901 gcacgcgctg gccgcgctgc gcacgccggt cgacgcgttc ttcgacagcg tgatggtgaa
      961 cgccgacgac atcgcgctgc gcgccaaccg cctcggcctg ctcgcgaccc tgcacgccgc
     1021 gatgaaccgc gtcgccgatc tctccaaact cgcgagctga gccgatgacc tcgatgaaac
     1081 tcatcatcct cgatcgcgac ggcacgatca acgaggaccg cgacgatttc gtgaagacgc
     1141 cggacgagtg ggtgccgatc cccggcgcgc tcgaggcgat cgcgcggctc aaccacgcgg
     1201 gctggcacac cgtggtcgcg accaaccagt cgggcctggg ccgcggcacc ttcgacatgg
     1261 ccacgctgaa cgcgatgcac accaagatga accagttgct cgcgaagcag ggcgggcgca
     1321 tcgacgcggt gttcttctgc ccgcacgcgc ccgaggagcc gtgccactgc cgcaagccgc
     1381 tgccgggcct gttcgagcag atcggcgagc gcttcggcgt ggcgctgcac aacgtgccgg
     1441 tggtcggcga ctcgctgcgc gacctgcagg ccggcgtcgc ggtcggctgc cagccgcacc
     1501 tggtgcgcac cggcaagggc gcgcgcatga gcccggcgca gctcgacgcg ctgtgcgcgc
     1561 aggtgccggg cacgcgcgtg catgccgatc tcaccgcgtt cgccgagtac atcgtgcgcg
     1621 aggagcgcaa gggtcgcacc gacaccggcg agtccgattc cggcttcggc aggctcgact
     1681 gatggctcat ttcttcaacg ccgtgcgctc ggcgctgttc gtgctgttca tggcggtcac
     1741 ggtgatcccg tgggcgctgg cggtgctgct ggtgtcgatc gtcgcgaagg gcaacgtcgt
     1801 ctactggatg tgcgtgggct ggctgcgcac cgcgatctgg ggcgcgaagg tgatctgcgg
     1861 cgtgcacgcg cggctgcacg ggttcgagaa cctgcctgat tcgccggtcg tgctgttgcc
     1921 caagcaccag tcgacctggg agaccttcgc gttcccgacg ctgatgccgc acccgctgtg
     1981 ctacgtgttc aagcgcgagc tgctgtacat cccgttcttc ggctgggcga tggcgcgcat
     2041 ggacatgatc cacatcgacc gcagcaagcg cgccgaggcc tggaacctgg tcgccgcgca
     2101 gggccgcaag ttcatggcca agggccactg ggtcatcatg ttccccgaag gcacgcgcat
     2161 cgcgcgcggc gcgaaaggcc actacaaggg cggcggcacg cggctggcca tacagaccga
     2221 gcggccggtg ctgccggtgg cggtgacctc ggcgcgctgc tggccgcgca agagcttcgt
     2281 gctgcgcccg ggcgtgatcg acgtgtcggt cggcaagccg atcccgtcga ccggccgcga
     2341 acccgacgag ctgatgcgcg aggtcgaggc ctggatcgag accgagatgc gccgcctcga
     2401 tccgcaggcc tacgccacgc cggccgcgcc cgcctgacag agccagcgat gagccgccgc
     2461 gccgccgccc tcgacacgca gcaactgagc ctgttcgagg agtcggcgcc gccggtggcg
     2521 ccgctggcgc cgatgcagac catcgaaccc gccgcgcccg gcgtcgaccg cgcgcccgcg
     2581 gccccgcccg ccgaggcggt ggccagtgcc ggcgggctcg cgcccgccgt cttccgccac
     2641 cc
//
LOCUS       DATMJB010000575         3850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_83116, whole genome shotgun sequence.
ACCESSION   DATMJB010000575 DATMJB010000000
VERSION     DATMJB010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83116"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VNU48_12240"
     CDS             complement(<1..962)
                     /locus_tag="VNU48_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HWI12092.1"
                     /translation="MSSAAAEVRPQPRRVWLFAVAGVLALVLLAVGYVQWRQFKLLDA
                     AAHYQNDALGWSFSQLESEQLRLRNALQLYVDDPVRTDADKVQLRYDIFVSRVSLVDH
                     ERAAGIMHDQPAYQPAMAQVRAFVKSADRYLGEAPATPLTPAGGAELLGPLDALNVPL
                     HDLSLGASHLLYQNATERNNAVRQQSELSIALTLFQCLLLLALAVIVLRQLRALTQRR
                     RSLEALAENLHGARQDAEAASRSKSAFLANMSHEIRTPFHGLLGMMSLLQEGGLSPQQ
                     AAYLDTARESAQHLLTILNDILDISQLESGRLQVAPQTVDLLQL"
     gene            complement(959..1486)
                     /locus_tag="VNU48_12245"
     CDS             complement(959..1486)
                     /locus_tag="VNU48_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWI12093.1"
                     /translation="MKYSTPRRGAILSLVAAGLLGNLAALPAHALDAPTGKVVLTVGG
                     SIANANDKGAASFDLTLLQQLPQTSFSTKTPWYAQPRKFSGVLLRDLLAGLGAAPGAT
                     IKAVALNDYRVDIPADDAARHGALLAYLLDDQPMSVREKGPLVIIYPFDDKPELRDAV
                     HYSRAIWQLRSLEIK"
     gene            complement(1549..3729)
                     /locus_tag="VNU48_12250"
     CDS             complement(1549..3729)
                     /locus_tag="VNU48_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020200738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWI12094.1"
                     /translation="MFKLTTLAGACALALAAAPAHAADDTLAQAAPAAPAPSASAPTT
                     QLRPVEVKGVRQRLDEARSSLSPDTGSTIYRFDSADIDKLPLGDATPLNQVLLRAPGV
                     VGDSYGQVHVRGDHANLQYRINGIVIPEAISGFGQVLDTRFADNINVLTGALPAQYGY
                     RTAGVVDIRTQGQPSAGGDAAFANHGSVDVVLGSRGHRETSANIGGSAGDLQYFVTGS
                     YLQNQIGVENPTPAKNAVHDDTRQTKGFAYLSYLLGDNSRVGLMLGTAQSRFQIPNVP
                     GESPAFTLAGSAPSDSLALDANQRERNSFQVLSFQSTLGDGTDYQIALFNRTSSVHYQ
                     PDPVGDLVYNGVAADVTRRNVASGLQLDASRALGANHTVRAGLFAQRERGSVANRSSV
                     FPADDDGNQTSDVPLTIDDDSRLGGHLVGAYLQDEWQPTKALTVNYGLRYDKVDTVTK
                     ESQWSPRLGAVYQLGADTRVHAGYARYFTPPPTEKIDTTSVQKFLATTNALPSDANTA
                     VRSERSNYYDLGVSHQLTPRITLGADAYYRQVQHLQDEGQFGNALIYSAFNFEKGRIF
                     GVELSASYRDERLSGYANIGFTSARGKGIETGQFNFDADELAYITQNWVHLDHEQTLA
                     ASAGVSYKFSDATSAGLDALYGSGLRRGFANTEHLPGFVQVNASVARSFDTAAVGKFE
                     LRLAAINLFDRSYELRDGSGIGVGAPQFAPRRTLYVGLSKPFTF"
BASE COUNT          526 a         1276 c         1436 g          612 t
ORIGIN      
        1 atcagctgca gcaggtcgac ggtctgcggc gccacctgca accggccgga ctcgagctgc
       61 gagatgtcga ggatgtcgtt caggatcgtc agcaggtgct gcgccgactc gcgtgcggtg
      121 tcgaggtagg ccgcctgctg cggcgacagg ccgccctcct gcagcagcga catcatgccg
      181 agcaggccgt ggaacggcgt gcggatctcg tggctcatgt tggcgaggaa ggcgctcttc
      241 gaacggctcg cggcctcggc gtcctgacgc gcgccatgca ggttctcggc cagcgcctcg
      301 aggctgcgcc ggcgctgcgt cagcgcgcgc agctggcgca gcacgatgac cgcgagcgcg
      361 agcagcagca ggcactggaa cagcgtcagc gcgatgctca gctcgctctg ctggcgcacc
      421 gcgttgttgc gctcggtcgc gttctggtac agcaggtgcg aggcgcccag cgacaggtcg
      481 tgcagcggca cgttcagcgc gtccagcggg ccgagcagct cggcgccgcc ggccggcgtc
      541 agcggcgtgg cgggtgcctc gccgaggtaa cggtccgcgc tcttgacgaa ggctcgcacc
      601 tgcgccatcg ccggctggta ggcgggctgg tcgtgcatga tgccggcggc gcgctcgtgg
      661 tcgaccaggc tgacgcggct gacgaagatg tcgtagcgca gctgcacctt gtcggcgtcg
      721 gtgcgcaccg ggtcgtcgac atacagctgc agcgcgttgc gcaaccgcag ctgctcgctt
      781 tcgagctgcg agaagctcca gcccagcgca tcgttctggt agtgcgccgc cgcatcgagc
      841 agcttgaact ggcgccactg cacatagccg acggccagca gcaccagcgc cagcacaccc
      901 gccacggcga acagccagac ccgccgcggc tggggccgga cctcggcggc ggccgagctc
      961 atttgatttc gagacttcgc agctgccaga tcgcgcggct gtagtgcacg gcgtcgcgca
     1021 gctcgggctt gtcgtcgaac gggtagatga tcaccagcgg gcccttctcg cgcaccgaca
     1081 tcggttgatc gtcgagcagg taggcgagca gcgcaccgtg gcgcgcggcg tcgtcggcgg
     1141 ggatgtcgac gcggtagtcg ttcagcgcca ccgccttgat cgtcgcgccc ggcgccgcgc
     1201 cgaggccggc aagcaggtcg cgcagcagca cgccgctgaa cttgcgcggc tgcgcgtacc
     1261 agggcgtctt ggtcgagaag ctggtctgcg gcaactgctg cagcagcgtc aggtcgaacg
     1321 acgcggcgcc cttgtcattg gcgttcgcga tgctgccgcc gacggtcagc acgaccttgc
     1381 cggtcggtgc gtcgagcgcg tgggccggca gcgccgccag gttgccgagc aggccggcgg
     1441 ccaccaggct cagaatcgcg ccgcggcgcg gggtcgaata cttcatcggc ctgattctgg
     1501 catggcgccg tacgcggccg aggcagggcc cgacgcccgg cgcccgcctc agaacgtgaa
     1561 cggcttgctc aggcccacgt acagcgtgcg ccgcggtgcg aactgcggcg cgccgacgcc
     1621 gatgccgctg ccgtcgcgca gctcgtagct gcgatcgaac aggttgatcg ccgccagccg
     1681 cagctcgaat ttgccgacgg ccgccgtgtc gaagctgcgc gcgaccgatg cgttgacctg
     1741 cacgaagccc ggcaggtgct cggtgttcgc gaagccgcgc cgcagcccgc tgccgtagag
     1801 cgcgtcgagg ccggcgctgg tcgcgtcgct gaacttgtac gacacgccgg ccgaggccgc
     1861 gagcgtctgc tcgtggtcga ggtggaccca gttctgcgtg atgtacgcga gctcgtcggc
     1921 gtcgaagttg aactgcccgg tctcgatgcc cttgccgcgc gccgacgtga agccgatgtt
     1981 ggcgtagccc gagagccgct cgtcgcgata cgacgccgag agttccactc cgaagatgcg
     2041 ccccttctcg aagttgaagg ccgagtagat cagcgcattg ccgaactggc cttcgtcctg
     2101 caggtgctgc acctggcggt agtacgcgtc ggcgccgagc gtgatgcgcg gcgtcaactg
     2161 gtgcgagacg ccgaggtcgt agtagttcga gcgctccgag cgcaccgcgg tgttggcgtc
     2221 ggacggcagc gcgttggtgg tggccaggaa tttctgcacc gaggtggtgt cgatcttctc
     2281 ggtcggcggc ggcgtgaagt agcgcgcgta gccggcgtgc acgcgggtgt cggcaccgag
     2341 ctggtagacg gcgcccaggc gcgggctcca ttgcgactcc ttcgtcaccg tgtcgacctt
     2401 gtcgtagcgc agcccgtagt tgacggtcag cgccttggtc ggctgccact cgtcctgcag
     2461 gtaggcgccg accaggtggc cgccgagccg gctgtcgtcg tcgatcgtca gcggcacgtc
     2521 gctcgtctgg ttgccgtcgt cgtcggccgg gaacacgctc gagcggttcg cgaccgagcc
     2581 gcgctcgcgc tgcgcgaaca gcccggcgcg aacggtgtgg ttcgcgccga gcgcgcggct
     2641 cgcgtcgagc tgcaggccgc tcgcgacgtt gcgccgcgtc acgtcggcgg cgacgccgtt
     2701 gtagaccagg tcgcccaccg gatcgggctg gtagtgcacg ctgctcgtgc ggttgaacag
     2761 tgcgatctgg tagtcggtgc cgtcgccgag cgtcgactgg aagctcagca cctggaagct
     2821 gttgcgctcg cgctggttgg cgtcgagcgc gagcgaatcg gacggcgcgc tgccggcgag
     2881 cgtgaacgcc ggcgattcgc ccggcacgtt cgggatctgg aagcggctct gcgcggtccc
     2941 gagcatcagg ccgacgcggc tgttgtcgcc gagcaggtag ctcaggtagg cgaagccctt
     3001 ggtctggcgc gtgtcgtcgt ggacggcgtt cttcgccggc gtcgggttct cgacgccgat
     3061 ctggttctgc aggtacgaac cggtgacgaa atactgcaga tcgccggcgc tcccgccgat
     3121 gttggcgctg gtctcgcggt ggccgcggct gccgagcacg acatcgacgc tgccgtggtt
     3181 cgcgaacgct gcgtcgccgc cggcgctcgg ctggccctgc gtgcggatgt cgacgacgcc
     3241 cgcggtgcgg taaccgtact gcgccggcag cgcgccggtc agcacattga tgttgtcggc
     3301 gaagcgcgtg tcgagcacct ggccgaagcc gctgatcgcc tcggggatca cgatgccgtt
     3361 gatgcggtac tgcaggttcg cgtggtcgcc gcgcacgtgc acctggccgt acgaatcgcc
     3421 gacgacgccg ggcgcgcgca gcagcacctg gttcagcggc gtcgcgtcgc ccagcggcag
     3481 cttgtcgatg tcggcgctgt cgaagcggta gatcgtgctg ccggtgtcgg gcgagagcga
     3541 gctgcgcgcc tcgtcgaggc gctggcgcac gcccttgacc tcgaccggcc ggagttgcgt
     3601 ggtcggcgcg ctggccgagg gggcgggtgc ggcgggcgcg gcctgcgcga gcgtgtcatc
     3661 ggccgcgtgc gcgggcgcgg cggccagggc cagcgcgcag gcgccggcca gggtggtcaa
     3721 cttgaacatg ggaatcctga tctgaaagcg gaacgcgcga gcgcgcgccc atcggcgcag
     3781 cgtcgcgggc gacggggctc agatcaggac gggtggggcg cgcgaacgaa acgccagccg
     3841 cagcgcggcc
//
LOCUS       DATMJB010000576         3679 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_616506, whole genome shotgun sequence.
ACCESSION   DATMJB010000576 DATMJB010000000
VERSION     DATMJB010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616506"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VNU48_12255"
     CDS             <1..497
                     /locus_tag="VNU48_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWI12095.1"
                     /translation="LTGVTKAWAAALTLSLGIALLLTAVTVAYKRVWQGVGLRLERWI
                     PEQRKPALTVACGLVLGVLVSLSSIGAGAIGATLILLLYPRLKSHEIVGTDIAHAVPL
                     TLVAGIGHATLGHVEWGLLGALLLGSIPGIWIGAQLTRTMPERVVRTLLCIALVSAGL
                     KVIT"
     gene            497..2200
                     /locus_tag="VNU48_12260"
     CDS             497..2200
                     /locus_tag="VNU48_12260"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWI12096.1"
                     /translation="MYQYTEFDKQFVHQRAAQFRDQLERHLAGKLPEDDFRALRLQNG
                     WYVQRHAPMARIAVPYGELSSAQLRTLARIAREFDRGYAHMTTRQNVQFNWIPLPRAA
                     DVMDALAAVDMHGIQTSGNCIRNITSDALAGIAPDEIVDPRPYAELLRQWSALQPEFA
                     FLPRKFKIAITGATEDRAAIGWHDIGLQLKKNDAGEVGFKVLVGGGMGRTPIIGITIR
                     EFLPWQQILVFIEAIVRVYNRYGRRDNAYKARIKILVKAEGQKFVDAVNAEFADILEN
                     DVAGAAHLLPQAELDRVAKSFELPAGVQARVDAAAPAFDAPKAFGRWLERNVHGHRIA
                     GYRAVTLSLKRTGQAPGDATDDQMDAAADIAERFSHGELRVTHEQNLLLPWVLERDLP
                     ALWQAARDASFATPNIGLLTDMIACPGGDFCALANARSIPIAEEITQRFDEIDELYDI
                     GNIDLHISGCINSCGHHHSGHIGVLGVDKDGKEWYQISLGGSDGSTQSGAAVPGKVIG
                     PSFAADEVADVIESLVETYRERRAAANELFIDTVKRVGIEPFKASANAVRRSTAIAHA
                     A"
     gene            2224..2769
                     /locus_tag="VNU48_12265"
     CDS             2224..2769
                     /locus_tag="VNU48_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HWI12097.1"
                     /translation="MKFIDPHKDQWHALGGEDGPAACIDPAPHLLLTVEQWHAFRAQW
                     PQGMPVGVMLANDAEVKEIAADLPRLALVQLSFPKWVDGRAYSQAHTLRARYRFKGEV
                     RATGEVLVDMVLLLARTGFDAAVLRADQSLDAAHRALTFFPGYYQGGVDDPLPLFAKP
                     PGTADALVRAQEEFVHGGAAI"
     gene            2766..3509
                     /locus_tag="VNU48_12270"
     CDS             2766..3509
                     /locus_tag="VNU48_12270"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWI12098.1"
                     /translation="MSSNAITLYARETPGFDARVASALALLQRAAAEHSGRVVQATSL
                     GAEDMVITDLIARHGLGIAIGTLQTGMLHAQTSALIPRIEARYALEVEVYEPVRESVV
                     HFVGRHGERAMYDSIELRKGCCAIRKVEPLARMLESRSAWVTGMRRDQSANRGAVEFT
                     ELDDKGRTKFNPLADWSWADVWHYIASNDVPYNPLHDEFMPSIGCAPCTRAIAVGEDF
                     RAGRWWWEQDGAKECGLHVHDESAVSLIG"
     gene            3515..>3679
                     /locus_tag="VNU48_12275"
     CDS             3515..>3679
                     /locus_tag="VNU48_12275"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829099.1"
                     /note="with CysN catalyzes the formation of
                     adenylylsulfate from sulfate and ATP; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase small subunit"
                     /protein_id="HWI12099.1"
                     /translation="MNAPLKVEQLLPELDHTHLDWLEEEAIFTLRETVAAFERPALLF
                     SGGKDSCVVLR"
BASE COUNT          601 a         1310 c         1248 g          520 t
ORIGIN      
        1 acctgaccgg cgtgaccaag gcctgggccg ccgcgctgac cctgtcgctg ggcatcgcgt
       61 tgctgttgac cgccgtgacg gtggcctaca agcgtgtctg gcaaggcgtc ggcctgcggc
      121 tggagcgctg gatccccgag cagcgcaagc ccgcgctgac ggtcgcatgc gggctggtgc
      181 tgggcgtgct ggtctcgctc agctcgatcg gcgccggcgc gatcggcgcg acgctgatcc
      241 tgctgctgta cccgcggctg aagtcgcacg agatcgtcgg caccgacatt gcccacgcgg
      301 tgccgctcac gctggtcgcg ggcatcggcc acgcgaccct gggtcacgtc gagtggggcc
      361 tgctcggcgc gctgctgctg ggctccatcc cgggcatctg gatcggcgcg caactgaccc
      421 gcacgatgcc cgagcgcgtg gtgcgcacgc tgctgtgcat tgcgctcgtc agtgccggat
      481 tgaaagtgat cacctgatgt accagtacac cgaattcgac aagcagttcg ttcatcaacg
      541 cgccgcgcaa ttccgcgacc agctcgagcg ccacctcgcg ggcaagctgc ccgaggacga
      601 cttccgcgcg ctgcgcctgc agaacggctg gtacgtgcag cgccacgcgc cgatggcgcg
      661 catcgcggtg ccgtacggcg aactgagctc ggcgcagctg cgcacgctgg cgcgcatcgc
      721 gcgcgagttc gaccgcggct acgcgcacat gacgacgcgc cagaacgtgc agttcaactg
      781 gatcccgctg ccgcgcgcag ccgacgtgat ggacgcgctg gccgcggtcg acatgcacgg
      841 catccagacc agcggcaact gcatccgcaa catcaccagc gacgcgctcg ccggcatcgc
      901 gcccgacgaa atcgtcgacc cgcggcccta cgccgaactg ctgcgccagt ggagcgcgct
      961 gcaacccgag ttcgcgttcc tgccgcgcaa gttcaagatc gcgatcaccg gcgccaccga
     1021 agaccgcgcg gcgatcggct ggcacgacat cggcctgcag ctgaagaaga acgacgccgg
     1081 cgaggtcggc ttcaaggtgc tggtcggcgg cggcatgggg cgcacgccga tcatcggcat
     1141 cacgatccgc gagttcctgc cgtggcagca gattctcgtc ttcatcgagg cgatcgtgcg
     1201 cgtctacaac cgctacggcc ggcgcgacaa cgcctacaag gcgcgcatca agatcctcgt
     1261 gaaagccgag ggccagaagt tcgtcgacgc ggtgaacgcc gagttcgccg acatcctcga
     1321 gaacgatgtg gcgggcgccg cgcacctgct gccgcaggcc gagctcgacc gcgtcgcgaa
     1381 gagcttcgag ctgccggccg gcgtgcaggc gcgggtcgat gcggcggcgc cggcgttcga
     1441 tgcaccgaag gccttcggcc gctggctcga acgcaacgtg cacggccacc gcatcgccgg
     1501 ctaccgcgcc gtgaccttgt cgctcaagcg caccgggcag gcgcccggcg acgcgaccga
     1561 cgaccagatg gacgccgcgg ccgacatcgc cgagcgcttc agccacggcg agctgcgcgt
     1621 cacgcacgag cagaacctgc tgctgccctg ggtgctcgag cgcgacctgc ccgcgctgtg
     1681 gcaggcggcg cgcgacgcca gcttcgccac gccgaacatc ggcctgctga ccgacatgat
     1741 cgcctgcccc ggcggcgact tctgcgcgct ggcgaatgcg cgctcgatcc cgatcgccga
     1801 agagatcacg cagcgcttcg acgagatcga cgagctctac gacatcggca acatcgacct
     1861 gcacatcagc ggctgcatca actcctgcgg ccaccaccac agcggccaca tcggcgtgct
     1921 cggcgtcgac aaggacggca aggagtggta tcagatctcg ctcggcggct ccgacggctc
     1981 gacgcagagc ggcgcggccg tgcccggcaa ggtgatcggc ccgtcgttcg cggccgacga
     2041 ggtggccgac gtgatcgagt cgctggtcga gacctaccgc gagcgccgcg ctgccgccaa
     2101 cgaactgttc atcgacaccg tcaagcgcgt cggcatcgag ccgttcaagg cgtcggcgaa
     2161 cgcggtgcgc cgcagcaccg cgatcgcgca cgccgcgtga cgcgcccgat tggaagcaac
     2221 gagatgaaat tcatcgaccc gcacaaggac cagtggcacg cgctcggtgg cgaggacggc
     2281 ccggccgcct gcatcgaccc ggccccgcat ctgttgctaa cggtcgagca atggcacgcg
     2341 tttcgcgcgc agtggccgca gggaatgccg gtcggcgtga tgctggccaa cgacgccgaa
     2401 gtcaaggaga tcgccgccga cctgccgcgc ctggcgctgg tgcagctgag cttcccgaag
     2461 tgggtcgacg gccgcgccta cagccaagcc cacaccttgc gcgcgcgcta ccgcttcaag
     2521 ggcgaggtgc gcgccaccgg cgaggtgctg gtcgacatgg tgctgctgct cgcgcgcacc
     2581 ggcttcgatg ccgcggtgct gcgcgccgac cagtcgctcg acgccgcgca ccgcgcgctg
     2641 accttcttcc cgggctacta ccagggcggt gtcgacgacc cgctgccgct gttcgcgaag
     2701 ccgcccggca cggccgacgc actggtgcgc gcacaggaag aattcgtcca cggcggagcc
     2761 gcgatatgag cagcaacgcg atcacgctct acgcgcgcga aacgccgggc ttcgacgcgc
     2821 gcgtcgccag cgcgctggcc ttgctgcagc gcgccgccgc cgagcattcg gggcgcgtcg
     2881 tgcaggccac cagcctgggc gccgaggaca tggtgatcac cgacctgatc gcgcgccacg
     2941 ggctcggcat cgcgatcggc accctgcaga ccggcatgct gcacgcgcag acctcggcgc
     3001 tgatcccgcg catcgaggcg cgctatgcgc tcgaggtcga ggtctacgag ccggtgcgcg
     3061 aatcggtcgt gcacttcgtc ggccgccacg gcgagcgcgc gatgtacgac agcatcgagc
     3121 tgcgcaaggg ctgctgcgcg atccgcaagg tcgagccgct ggcacgcatg ctcgagagcc
     3181 gcagcgcctg ggtcaccggc atgcggcgcg atcagtcggc gaaccgcggc gcggtcgagt
     3241 tcaccgaact cgacgacaag ggccgaacca agttcaaccc gctggccgac tggtcgtggg
     3301 ccgacgtgtg gcactacatc gcatcgaacg acgtgcccta caacccgctg cacgacgagt
     3361 tcatgcccag catcggctgc gcgccctgca cgcgcgcgat cgcggtcggc gaggacttcc
     3421 gcgccggccg ctggtggtgg gagcaggacg gcgcgaagga atgcggcctg cacgtccacg
     3481 atgaatccgc cgtttcgttg atcggatgac ccgcatgaac gcaccgttga aagtcgaaca
     3541 gctgctgccc gagctcgacc acacgcatct cgactggctc gaagaggaag cgatcttcac
     3601 gctgcgcgag accgtcgccg cgttcgagcg ccccgccctg ctgttctcgg gcggcaagga
     3661 ctcgtgcgtc gtgctgcgc
//
LOCUS       DATMJB010000577         2809 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_347548, whole genome shotgun sequence.
ACCESSION   DATMJB010000577 DATMJB010000000
VERSION     DATMJB010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347548"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1634)
                     /locus_tag="VNU48_12280"
     CDS             complement(117..1634)
                     /locus_tag="VNU48_12280"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWI12100.1"
                     /translation="MNELVLRVEGLSKRYGAVRALDGVSLELHRHEVVGVIGANGAGK
                     STLLGVLAGRCRPDGGRIVLRGEALTLKSAAAAADAGIGMVFQEQSLLPNLSVAENIL
                     LGHEDAALRAGFYDWRALNALAAAQLAKLGSRIAPSARTDSLSFAERQVVELAKVLAL
                     EDRTRHAPVILLDEPTSMLDPGQVEVVLAQVERLRARASVLFVSHRLDEVLRVCDRVY
                     VLSHGRCVAQRRREDCEPAELQRLMLGHELGAAGPVAAVRTPAPPAPARLSVRGLGRA
                     HSYQAVSFELGAGEVLGIAGAEGSGRESLCRTLFGAEAPDRGEIVLDGQAVRLGGPAD
                     AVRLGIGYVPAERHAEGLVAGLSVLENMTLARLGDMRRGPFIDRVRERALVNRWIDRL
                     RIKPGAPQTPVEQLSGGNQQKLVLAKWLIARDPKILILDRPLRGLDVGARGEIIHLIR
                     ELAARGVGILVVADTLDELAAMSDSVIVMRDGAVCGRFPACEAKPSDLEILERMV"
     gene            complement(1631..2587)
                     /locus_tag="VNU48_12285"
     CDS             complement(1631..2587)
                     /locus_tag="VNU48_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI12101.1"
                     /translation="MKAASLRPWGPFIALLVLCAALALAEPRFASAQNLFVIADRSAV
                     PLILAVGMTFVVLQGSIDLSIEGVMATSSLSVALLVLNSRNGLDLGFAGVAAGMLAGA
                     LIGLLNGVLVTRLRVPSFMATLGVSSAAMGLAMLLSGGQPPLLRDLALRSFGLGRTWG
                     APNLAIVALLVLVLGWVLQNHTRFGRYSLAIGGAEGLARLSGLPVDRYKVLAFAFCGL
                     LAGLGGALESARIGLGHVEIGIGQMFTTVTAVVIGGTPLSGGRGGVLQSAVGALMLAV
                     LANGMIFVGITPDIQKAVHGAIILSVTLFATWQMRSRLRVVP"
     gene            complement(2584..>2809)
                     /locus_tag="VNU48_12290"
     CDS             complement(2584..>2809)
                     /locus_tag="VNU48_12290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12102.1"
                     /translation="YYQGSIGLAMGVCVLTGKLPPPSAWPKEKREFYLRLQVITKDNA
                     LQFQAEPQASAYAAEWDCDHLWSRSTGKAF"
BASE COUNT          467 a         1043 c          918 g          381 t
ORIGIN      
        1 gcagcagcat gccccgccgg cccgatcccg cgccgatacg tgcacggcgg acattcaccg
       61 acagcatcgg gccgcgagcc gacacaccat cacaactgca tgcggccagt ttcacttcag
      121 accattcgtt ccaggatttc cagatcggac ggcttggctt cgcaggccgg gaagcggccg
      181 cagaccgcac cgtcgcgcat cacgatgacg ctgtcgctca tcgccgcgag ttcgtccagc
      241 gtgtcggcga cgaccaggat gccgacaccg cgggcggcga gttcgcgtat caaatgaatg
      301 atctcccctc gggcgccgac gtcgagccca cgcagtggcc ggtccaggat gaggatcttc
      361 ggatcccgtg caatcagcca tttcgccagc acgagcttct gctggttgcc accgctgagt
      421 tgttcgaccg gcgtctgcgg cgcacccggc ttgatccgca gccgatcgat ccagcggttc
      481 accagggctc gctcgcgaac gcggtcgatg aacgggccgc ggcgcatgtc gccgagccgc
      541 gccagcgtca tgttctcgag cacgctcagg ccggcgacga ggccctcggc gtgccgctcc
      601 gcgggcacat agccgatgcc gagccgcacc gcgtcggcgg gcccgccgag gcggaccgct
      661 tgcccgtcga gaacgatctc tccgcggtcg ggcgcctcgg caccaaacag cgtgcggcac
      721 aggctctcgc gcccggagcc ttcggcgccc gcgatgccga gcacttctcc ggcgcccagc
      781 tcgaagctca ccgcctggta gctgtgcgca cggccgaggc ctcgcaccga caagcgggcc
      841 ggtgcgggcg gcgccggggt gcgcaccgcc gccaccgggc cggcggcacc cagctcgtgt
      901 cccagcatca ggcgctgcag ctcggccggc tcgcagtctt cgcgccgacg ctgcgcaacg
      961 cagcggccat ggctcaacac gtagacgcgg tcgcagacgc gcagcacctc gtcgagccgg
     1021 tgcgacacga acaggacgct ggcgcgcgcg cgcaagcgct cgacctgcgc cagcacgact
     1081 tcgacctggc ccgggtccag catggatgtc ggctcgtcga gcaggatgac gggcgcgtgc
     1141 cgcgtgcggt cctcgagcgc cagcaccttg gccagttcga ccacctgccg ttcggcgaac
     1201 gacaggctgt cggtgcgcgc cgatggcgcg atgcgggaac ccagcttggc cagctgcgcc
     1261 gcggccagcg cattcagcgc acgccagtcg tagaagccgg ctcgcaacgc cgcatcctcg
     1321 tggccgagca ggatgttttc cgcgacgctg aggttgggca gcagcgactg ttcctggaac
     1381 accatgccga tgcccgcatc ggcggccgcg gctgcgctct tcagcgtcag ggcttcgccg
     1441 cgcagcacga tgcgcccgcc atcgggccgg caccgaccgg ccagcacccc gagcagcgtc
     1501 gacttgccgg cgccgttcgc gccgatcacg ccgacgactt cgtgcctgtg cagctcgagc
     1561 gagacaccgt cgagcgccct cactgcgccg tagcgcttgc tcaatccctc gactcgcaag
     1621 accagctcgt tcacgggacg acccgcagcc ggctgcgcat ctgccaggtg gcgaacagcg
     1681 tcaccgacag gatgatcgcg ccatgcaccg ccttctggat gtcgggcgtg atgccgacga
     1741 agatcatgcc gttggccagc acggccagca tgagcgcgcc cacggcagac tgcaggacgc
     1801 cgccacgccc gccgctgagc ggcgtgcccc cgatcaccac ggcggtgacc gtggtgaaca
     1861 tctgcccgat gccgatctcg acgtgaccca gcccgatgcg tgccgactcc agcgccccgc
     1921 ccagcccggc cagcagtccg cagaaggcga acgccagcac cttgtagcga tcgaccggca
     1981 ggccggacag gcgggccagc ccttccgcgc caccgatcgc caggctgtag cggccgaatc
     2041 gggtgtggtt ctgcagcacc cagccgagca ccaggaccag cagggcgacg atggccaggt
     2101 tcggtgcgcc ccaggtccgc cccaggccga agctgcgcag ggccaggtcc cgcaacagcg
     2161 gtggctggcc gccggacaag agcattgcca gccccatcgc cgccgacgag acgcccagcg
     2221 tggccatgaa cgacggcacc cgcaaccgcg tgaccagcac cccgttcagc aggccgatga
     2281 gcgcgcccgc cagcatccct gccgccacgc ccgcgaagcc gaggtcgagc ccgttgcggc
     2341 tgttgagcac gagcaaggcg acgctgagcg aactggtcgc catgacgcct tcgatcgaca
     2401 ggtcgatgga gccctgcagc accacgaagg tcatgcccac ggccaggatc aacggcacgg
     2461 cactgcggtc cgcgatcacg aacaggttct gcgcgcttgc gaaccgcggc tcggcgagcg
     2521 cgagcgctgc gcacagcacc agcagcgcga tgaaaggccc ccaggggcga aggctcgccg
     2581 ccttcaaaac gccttgccgg tgctgcggct ccagagatga tcgcaatccc attcggcagc
     2641 ataggccgat gcctgtggtt cagcctggaa ctgcaacgcg ttgtccttcg tgatgacctg
     2701 cagcctcaga tagaactcgc gcttctcctt cggccaggcc gagggcggcg gcagcttgcc
     2761 ggtgagcacg cacacgccca tcgccaggcc gatgctgccc tggtagtac
//
LOCUS       DATMJB010000578         3907 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_869757, whole genome shotgun sequence.
ACCESSION   DATMJB010000578 DATMJB010000000
VERSION     DATMJB010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869757"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VNU48_12295"
     CDS             <1..261
                     /locus_tag="VNU48_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12103.1"
                     /translation="LQMAGDTVHDAFGLMLHGIDGANAITQTTVTANADLLHGALKMS
                     GDQAEQSMATLKDLKSADVRVLAMVGIAVVGLGAVTLFQRKG"
     gene            264..920
                     /locus_tag="VNU48_12300"
     CDS             264..920
                     /locus_tag="VNU48_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12104.1"
                     /translation="MQQTVNLTAGNRARVNLAGSSFVMISTGVATNVELWIMAGTQEL
                     EYIRTAPRGFKARTPAAKFSHVEMRSSVDAVCEIVISDGLVDFDFITGTNVQATLLGL
                     PIPVSNDRGTPGNLLYVSGVSIGDAPATSTPDNAAVACTAVQALILAADATRRQITFT
                     NIGTDPVVLGSTGITWAKRCVILASGDSYLEDRAANLAWYGICDAAKTASVTTKTVLA
                     "
     gene            917..2554
                     /locus_tag="VNU48_12305"
     CDS             917..2554
                     /locus_tag="VNU48_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12105.1"
                     /translation="MSAFARRFANAVEVQAAVDASQAAQDTAYGDAADVAKGIAKVGG
                     AARVVNSVAVLKTLPKTGAPRAFLTGYYAAGDGGGGHYWYDAADVASADNGGTIIVAA
                     DGGRWKLVDSGAVSAMQFGAKGDGSNDGPALLAAQAAVSFVHLPKGRTFYSASALNGA
                     QFYGAGTLASPGETYAVSAWPRTGAGPVTNYNAWTFGSYENAVAASFTINSGAGQGKT
                     NTQILGTSGAGYATTPIGIARDHVAMYVEAHTFDPVVLGASAVYTANTVTANEVGPNA
                     AKIRQGVIIDTLHATPYTGTVQSVAGNVITVDAWYLQSGAPVAGTPANATGAKVNPNT
                     KVWAHNANLFVGGNAGNKQARTGTGFELGISVDPTIGVNVWGYDAVTLAGKPEAHFIS
                     RGSRSYGFLAQAGADFGHTSDSNGRGFYSRNDTIPFEALQGAISVWSVGVDGSTQSLG
                     IKRQGIPPSQCTNAAFVNCGPLPAAYGGLVWVSGFNAVGGAEGNFLVMVRSGAVALVS
                     VSDGTGLAITFQAAGRQLQIKCANAGTFQFIGHAFAA"
     gene            2551..3231
                     /locus_tag="VNU48_12310"
     CDS             2551..3231
                     /locus_tag="VNU48_12310"
                     /inference="COORDINATES: protein motif:HMM:NF013152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HWI12106.1"
                     /translation="MKPAQALVIVAAGALLIASRRTDAGAIEAADGDFGSFDLGTLTD
                     RAESFYNQLTETESTVETDTASANIGAGLTTIAQSEGTEARGAYRVCYGYGHTIADLS
                     DHPYLTKEWPGVVLSDQMCSNAGFGPGCKSTAAGRYQITATTWRRLGGVAKYGSFDEG
                     AQDAAAIDLIAGRGALEDLKAGRFENFVHKCRGEWASLPGNSAGQGQRTMTQLASWFT
                     DAGGALLA"
     gene            3228..3449
                     /locus_tag="VNU48_12315"
     CDS             3228..3449
                     /locus_tag="VNU48_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12107.1"
                     /translation="MNSNNLMTVGAVGFAGFALWWITRKPGGAVTTQPAQQQRDIGLN
                     SWNDLLTGQAALGTSAGFYSADQLANLFK"
     gene            3446..3604
                     /locus_tag="VNU48_12320"
     CDS             3446..3604
                     /locus_tag="VNU48_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12108.1"
                     /translation="MNITETHVLAALAIALIAYQLGKKQTQSAPAVAQTNIAAPADWW
                     SYAGTWGM"
     gene            3607..>3907
                     /locus_tag="VNU48_12325"
     CDS             3607..>3907
                     /locus_tag="VNU48_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12109.1"
                     /translation="MDVRTLQLAAVGAAAAVAAVLAYKVMNSAKQAADAAAGLLTGNN
                     AITENQTNAAGEKTTAYQGAGVLGTMGAATNSASGGVFASWGEDLGGWFYGATHTD"
BASE COUNT          684 a         1344 c         1322 g          557 t
ORIGIN      
        1 ctgcagatgg ccggcgacac cgtgcacgac gccttcgggc tgatgctgca cggcatcgac
       61 ggcgcgaacg cgatcacgca gaccaccgtc acggcgaatg ccgacctgct gcatggcgcg
      121 ctcaagatgt ccggcgacca ggccgagcag agcatggcga cgctcaagga cctcaagagc
      181 gccgacgtgc gcgtgctcgc gatggtcggt attgccgtcg tcggtctcgg cgccgtgacg
      241 ctctttcaac gcaagggctg accatgcaac agaccgtcaa cctcacggca ggcaatcgcg
      301 cccgcgtcaa cctggccggc tcatcgttcg tcatgatctc gaccggcgtc gcgacgaacg
      361 tcgagctgtg gatcatggcc gggacgcagg agctcgagta catccgcacc gcgccgcgcg
      421 gcttcaaggc acgcacgccg gccgcgaagt tttctcacgt cgaaatgcgg tccagcgtcg
      481 acgcggtgtg cgagatcgtc atctctgacg gcctggtcga cttcgatttc atcaccggca
      541 cgaacgtgca ggcgacgctg ctcggcctgc cgatcccggt cagcaacgat cgcggcacgc
      601 cgggcaatct gctctacgtg tcgggcgtgt cgatcggcga cgcgccggcg acgtcgaccc
      661 cggacaacgc ggccgtcgcc tgcaccgcgg tgcaagcact catcctcgcg gccgacgcga
      721 cgcgccggca gatcacgttc acgaacatcg gcaccgaccc ggtcgtgctc ggctcgaccg
      781 ggatcacctg ggccaagcgc tgcgtgatcc tggccagcgg tgacagctac ctcgaggacc
      841 gcgccgcgaa cctcgcgtgg tacggcatct gcgacgcggc caagaccgcc agcgtcacga
      901 ccaagacggt gctcgcgtga gcgccttcgc tcggcgcttc gccaatgcgg tcgaggtgca
      961 ggccgcggtc gatgcctcgc aggccgcaca ggacacggcc tacggggacg cggccgacgt
     1021 ggcgaagggt atcgccaagg tcggcggcgc cgcgcgcgtg gtgaactcgg ttgcggtgct
     1081 caagacgctg ccgaagaccg gcgcaccgcg cgccttcctg accggctact acgccgccgg
     1141 cgatggcggc ggcgggcact actggtatga cgcggccgac gtggcgagcg ccgacaacgg
     1201 cgggacgatc atcgttgcgg ccgacggcgg gcgctggaag ctggtcgaca gtggcgcggt
     1261 gtcggcgatg cagttcggcg cgaagggtga cggctcgaac gacgggccgg cgctgctcgc
     1321 ggctcaggcc gcggtgtcgt tcgtgcatct gccgaagggc cgcaccttct acagcgcatc
     1381 ggcgctgaat ggcgcgcagt tctacggcgc cggcacgctc gcctcgcccg gcgaaaccta
     1441 tgcggtgtcg gcgtggccac gcaccggagc cggcccggtc acgaattaca acgcgtggac
     1501 gttcggcagc tatgaaaacg cggtcgccgc gtcgttcacg atcaacagcg gcgcggggca
     1561 gggcaagacg aacacgcaga ttctcggcac gtcgggcgcg ggctacgcga cgacgcctat
     1621 cggcatcgcg cgcgatcatg tcgcgatgta cgtcgaggcg cacacgttcg acccggtcgt
     1681 gctcggcgcg tcggcggtct acaccgcgaa caccgtgacg gcgaacgagg tcggcccgaa
     1741 tgccgcgaag attcggcaag gcgtcatcat cgacacgctg cacgcgacgc cctacaccgg
     1801 caccgtgcaa tcggtcgccg gcaacgtcat cacggtcgac gcgtggtatc tgcaaagcgg
     1861 ggcgccggtt gccggcacgc cggccaatgc caccggcgcg aaggtcaacc cgaacacgaa
     1921 ggtctgggcg cacaacgcga acctgttcgt aggcggcaac gcgggcaaca agcaagcacg
     1981 caccggcacc ggctttgaac tcgggatctc ggttgacccg acgatcggcg tgaacgtgtg
     2041 ggggtatgac gcggtcacgc tggccggcaa gccggaagcg cacttcattt cgcgcggctc
     2101 gcgcagctac ggctttctcg cgcaggccgg cgccgacttc ggccacacca gcgacagcaa
     2161 cgggcgcggc ttctactcgc gcaacgacac gattccattc gaggcgctgc aaggcgcgat
     2221 cagcgtctgg tctgtcggtg tcgatggctc gacgcaatcg ctcgggatca agcgccaggg
     2281 cattccgcca tcgcaatgca caaatgccgc gttcgtcaac tgcggcccgc tgcctgcagc
     2341 ctatggcggg ctcgtgtggg tgtcgggctt caacgcggtc ggcggcgcag aaggaaactt
     2401 cctggtgatg gtgcgctcgg gcgctgtggc gctggtgtcc gtcagcgacg gcaccggcct
     2461 tgcgatcacg tttcaagcgg cgggccggca attgcagatc aagtgtgcca acgccggcac
     2521 ctttcaattc atcggccatg ccttcgcagc atgaagcccg cgcaagccct cgtcatcgtc
     2581 gcggccggcg cgctgctgat cgcctcgcgg cgcaccgacg ccggcgcgat cgaggcggct
     2641 gatggcgact tcggcagctt cgacctgggc acgctgaccg accgcgccga gtccttctac
     2701 aaccaactca ccgagaccga atccaccgtg gaaaccgaca ccgcatccgc caacatcggc
     2761 gcaggcctga cgaccatcgc gcagtccgaa ggcaccgagg cgcgcggcgc ctaccgcgtg
     2821 tgctacggct acgggcacac gatcgccgac ctgtccgacc atccctatct cacgaaggaa
     2881 tggcccggcg tggtcctgtc cgatcagatg tgctcaaacg ccggcttcgg tcccggctgc
     2941 aagtccaccg cggcgggccg gtaccagatc accgcgacga cctggcggcg cctcgggggc
     3001 gtcgccaagt acggcagctt tgacgaaggc gcgcaggacg cggcggctat cgacctgatc
     3061 gccgggcgcg gtgcgctcga agacctgaag gcgggccgct tcgagaattt cgttcacaag
     3121 tgccgcggcg aatgggcatc cctgccaggc aacagcgcgg gccaagggca acgcaccatg
     3181 acgcagctgg cgagctggtt taccgacgca ggaggcgcgc ttctcgcatg aactcgaaca
     3241 acctcatgac cgtgggcgcg gtcggcttcg ccggcttcgc gctctggtgg atcacccgca
     3301 agccaggcgg cgccgtcacg acgcaaccgg cgcagcaaca gcgagacatc gggctcaaca
     3361 gctggaacga cctgctgacg ggccaggccg cgctgggcac gtcggcgggc ttttactcgg
     3421 cagaccaact agcgaacctc ttcaaatgaa catcaccgaa acgcacgtgc tcgccgcgct
     3481 cgccatcgcg ctcatcgcgt accagctggg caagaagcaa acgcagagcg cgcccgcggt
     3541 cgcgcagacg aacatcgccg cgccggccga ctggtggagc tacgccggca cctggggcat
     3601 gtgatggtgg acgttcgcac cctgcagctg gcggcggtcg gcgccgccgc ggccgtcgcc
     3661 gcggtgctgg cctacaaggt catgaacagc gccaagcaag cggccgacgc ggcggcgggc
     3721 ctgctcaccg gcaacaacgc gatcaccgag aaccagacca acgccgccgg cgagaagacg
     3781 acggcctacc aaggcgcggg cgtgctcggc accatgggcg ccgcgacgaa ctcagcctcg
     3841 ggtggcgtgt tcgcgagctg gggcgaagat ctcggcggct ggttctacgg ggcgacgcac
     3901 accgacc
//
LOCUS       DATMJB010000579         3534 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_261163, whole genome shotgun sequence.
ACCESSION   DATMJB010000579 DATMJB010000000
VERSION     DATMJB010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261163"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..893)
                     /locus_tag="VNU48_12330"
     CDS             complement(30..893)
                     /locus_tag="VNU48_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWI12110.1"
                     /translation="MIRLQLSLQLDYEVFAPGCDFVFNIHAATTARQRVLDEALTLSP
                     YLPSRIETEPATGNRYLRLTAAPGPLQVRYRATVEIDHHIQLPATIGEVPVARLPAAV
                     LGYIYPSRYCQSDRLHKFAMREFGSRWQGYSRVQGIRDWVLERTTFSSNTSNSQTSAL
                     DTLLEQVGVCRDFAHLMIALCRAINIPARFATGVDYGADPALGPTDFHAYVEAYLGDR
                     WYMFDPSGTAIPMGFVRFGTGRDAADVAFATIFGAVQTAAPVIAIEAVAGADGRVVVP
                     YHRVEALSTDG"
     gene            complement(890..1111)
                     /locus_tag="VNU48_12335"
     CDS             complement(890..1111)
                     /locus_tag="VNU48_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12111.1"
                     /translation="MTTHHAPIDTEALRNELLVTVDALQRRRADLVSEALIAGYIARY
                     WMEWNGGTLRLTQTGKNVCDQLRAAARTP"
     gene            1345..3435
                     /gene="ppk1"
                     /locus_tag="VNU48_12340"
     CDS             1345..3435
                     /gene="ppk1"
                     /locus_tag="VNU48_12340"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HWI12112.1"
                     /translation="MTDAAAESPMPALLNRERAILEFNRRVLAQARRADVPLLERLRY
                     VCIVSSNMDEFFEVRFADYLEAARRAGSGVSMRDLQSVASDAHALIDDQYAAFNDEVM
                     PALRQQGIVILSHSDRDAAQRRWVAEFFEREVRPLLVPVGLDPSHPFPQVANKSLNFI
                     ARLSGRDAFGRENAIAIVKVPRVLPRVIRLPDALAGGEAPGRQAFVLLTSVIRAHLAE
                     LFPGRAVEVFSQFRVTRDSDLEVDEDDVKNLRQALRSGLTTRHFGQALRLEVVSTCPP
                     ALYEFLLQQFALPPAALYRVNGPVNLVRLFQLIDQADDDSERPAWRFAPFEPAWPQAA
                     LPREESIFDALRERDVLLHHPFESFEPVVQFLREAVHDPDVLAIKQTIYRTGSESPLM
                     DLLIDAARRGKEVMVVVELKARFDEEANINWAERLEAVGVQVVYGIVGLKTHAKLLLI
                     TRRERGRLHRYVHLSTGNYNPKTATQYTDLGFFTAEADLTRDADAVFQQLASLGRTKP
                     LRALLQAPFTLHKHMLAQIEQVAAAARAGRRARIVVKVNALTDEALIHALVRAGQQGA
                     DIDLIVRGACMLAPGVPGATERIRVRSVVGRFLEHSRVCYFCWGDTAADEALFLSSAD
                     WMSRNMFGRIEIAWPVRDAVLRQRVIDECLVPYLHDRQDAWQLQADGSYARIGSDGPS
                     AQQALVQRYAPGAA"
     gene            3435..>3534
                     /locus_tag="VNU48_12345"
     CDS             3435..>3534
                     /locus_tag="VNU48_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWI12113.1"
                     /translation="MDLILWRHAEAFEMREVDDDLARALTPKGERQA"
BASE COUNT          510 a         1215 c         1279 g          530 t
ORIGIN      
        1 cgcggcagca gacaccgcgc gccgagcgtc tatccgtcgg tcgacagcgc ttcgacccgg
       61 tggtagggca cgaccacgcg cccgtcggcg ccggcgaccg cctcgatcgc gatcaccggc
      121 gcggcggtct gcaccgcgcc gaagatcgtc gcgaaggcga cgtcggcggc gtcgcgcccg
      181 gtgccgaagc gcacgaagcc catcgggatc gcggtgccgg acgggtcgaa catgtaccag
      241 cggtcgccga ggtaggcctc gacgtaggcg tggaagtcgg tcggcccgag cgcggggtcg
      301 gcgccgtagt cgacgccggt cgcgaagcgc gccgggatgt tgatcgcgcg gcacagcgcg
      361 atcatcaggt gcgcgaagtc gcggcacacg ccgacctgct cgagcagcgt gtcgagtgcc
      421 gaggtctgcg agttgctcgt gttcgacgag aaggtcgtgc gctcgagcac ccagtcgcgg
      481 atgccctgca cgcggctgta gccctgccag cgcgagccga actcgcgcat cgcgaacttg
      541 tgcagccggt cggactggca gtagcggctc gggtagatgt agcccagcac cgcggccggc
      601 aggcgcgcca ccggcacctc gccgatcgtc gcgggcagct ggatgtggtg gtcgatctcg
      661 acggtggcgc ggtaccgcac ctgcagcggg ccgggcgcgg cggtgaggcg caggtagcgg
      721 ttgccggtgg cgggctcggt ctcgatgcgc gacggcaggt acgggctcag cgtcagcgcc
      781 tcgtcgagca cgcgctgccg cgcggtggtc gcggcatgga tgttgaagac gaaatcgcac
      841 ccgggcgcga acacctcgta gtcgagctgg agcgacagct gcagccggat catggcgtgc
      901 gcgccgcggc acgcaactgg tcgcagacgt tcttgccggt ctgcgtgagg cgcaaggtgc
      961 cgccgttcca ctccatccag tagcgcgcga tgtagccggc gatcagcgct tcgctgacga
     1021 ggtcggcgcg gcggcgctgt aaggcatcga cggtgaccag caactcgttg cgcagcgctt
     1081 cggtgtcgat cggtgcgtgg tgtgtcgtca atgcggctcc cgtgatggcc cacgttacca
     1141 ccggatcgag gcgcggcgtc gatccggcgg ccaataattg accggcggcg gctgggcgag
     1201 tcgataagcc tcggtctgag cgcggcccac gccggcccgc acgcggcccg cgtccgtgtg
     1261 ttccggttga accacgacgt cactcggtga cggaggcgcc gcgaccgtca cgaagctgtc
     1321 gcaaatcctt cataaactcg ccgcatgacc gacgccgccg ccgaatcgcc gatgccggcg
     1381 ctgctgaacc gcgagcgcgc gatcctcgag ttcaaccggc gcgtgctcgc gcaggcgcgc
     1441 cgcgccgacg tgccgctgct ggaacggctg cgctacgtct gcatcgtctc gtcgaacatg
     1501 gacgagttct tcgaggtccg cttcgccgac tacctcgagg ccgcgcgtcg cgccggttcg
     1561 ggcgtctcga tgcgcgatct gcaatcggtc gcttccgacg cgcacgcgct catcgacgac
     1621 cagtacgcgg ccttcaacga cgaggtcatg ccggcgctgc gccagcaggg catcgtgatc
     1681 ctcagccaca gcgaccgcga cgcggcgcag cgccgctggg tcgccgagtt cttcgagcgc
     1741 gaggtccggc cgctgctggt gccggtcggg ctggacccgt cgcacccgtt tccgcaggtc
     1801 gcgaacaagt cgctgaactt catcgcgcgg ctcagcggtc gggacgcatt cggccgcgag
     1861 aacgccatcg cgatcgtcaa ggtgccgcgc gtgctgccgc gcgtgatccg cctgcccgac
     1921 gcgctggcgg gcggggaggc accgggccgc caggccttcg tgctgctgac cagcgtgatc
     1981 cgcgcccacc tggccgagct gtttccgggc cgcgcggtcg aggtgttctc gcagttccgc
     2041 gtcacgcgcg actcggacct cgaggtcgac gaggacgacg tcaagaacct gcgccaggcg
     2101 ctgcgttcgg gcttgacgac gcgccatttc gggcaggcgc tgcggctcga ggtggtcagc
     2161 acctgcccgc ccgcgctcta cgaatttttg ctgcagcagt tcgcactgcc gccggcggcg
     2221 ctgtaccgag tcaacggtcc ggtgaatctg gtgcggctgt tccagctgat cgaccaggcc
     2281 gacgacgact cggagcgccc ggcctggcgc ttcgcgccgt tcgagccggc ctggccgcag
     2341 gccgcgctgc cgcgcgagga gtcgatcttc gacgcgctgc gcgagcgcga cgtgctgctg
     2401 caccatccgt tcgaatcgtt cgagccggtc gtgcagttcc tgcgcgaggc ggtgcacgac
     2461 cccgatgtgc tcgcgatcaa gcagacgatc taccgcaccg gcagcgagtc gccgctgatg
     2521 gacctgctga tcgacgcggc gcggcgcggc aaggaggtga tggtcgtggt cgagctgaag
     2581 gcgcgcttcg acgaggaggc caacatcaac tgggccgagc gcctcgaggc ggtcggcgtg
     2641 caggtggtct acggcatcgt cgggctgaag acgcatgcga agctgctgct gatcacgcgc
     2701 cgcgagcgcg ggcgcctgca ccgctacgtg cacctgtcca ccggcaacta caaccccaag
     2761 accgcgacgc agtacaccga cctgggcttc ttcaccgccg aggccgacct gacgcgcgac
     2821 gccgacgcgg tgttccagca gctcgcgagc ctgggtcgca ccaagccgct gcgcgcgctg
     2881 ctgcaggcgc cgttcacgct gcacaagcac atgctcgcgc agatcgagca ggtcgcggcc
     2941 gcggcgcggg ccgggcggcg cgcgcgcatc gtcgtgaagg tcaacgcgct gaccgacgag
     3001 gcgctgattc acgcgctggt gcgggccggg caacagggtg ccgacatcga cctgatcgtg
     3061 cgcggggcct gcatgctggc gccgggtgtg cccggcgcga ccgagcgcat ccgcgtgcgc
     3121 tcggtggtcg ggcgtttcct cgagcattcg cgcgtctgct acttctgctg gggcgacacg
     3181 gcggccgacg aggcgctgtt cctgtcgagc gccgactgga tgagccgcaa catgttcggc
     3241 cgcatcgaga tcgcctggcc ggtgcgcgac gccgtgctgc gccagcgcgt catcgacgaa
     3301 tgcctggtgc cctacctgca cgaccggcag gatgcctggc agctgcaggc cgacggcagc
     3361 tacgcgcgca tcggcagcga cgggccgagc gcgcagcagg cgctggtcca gcgctatgcc
     3421 ccgggagccg cctgatggat ttgatcctgt ggcgccacgc cgaggccttc gagatgcgcg
     3481 aggtcgacga cgatctggcg cgcgcgctca cacccaaggg cgagcgccag gcgc
//
LOCUS       DATMJB010000580         5637 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_764858, whole genome shotgun sequence.
ACCESSION   DATMJB010000580 DATMJB010000000
VERSION     DATMJB010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764858"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="VNU48_12350"
     CDS             <1..1408
                     /locus_tag="VNU48_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HWI12114.1"
                     /translation="LYYLLGNIDALGKYDGLLGVPALRFFGIELNTGRSFFYLLWTIV
                     VLAALAANNLLDSRAGRALRAVKGGSTMAEAMGVDSFRTKVTAFVLAALLASISGWLF
                     AHFQRTVNPTPFGLNMGIEYLFMAVLGGVAHVWGALMGAGVVVILKDQLQVWLPKLLG
                     GSGNYEIIVFGILLVLVLQYARDGLWAFIEARLPRVQRKVDWLDAPALPERERPPHGE
                     VVLDLKDARKEFGGLVAVNDVSFQVRAGEILGLIGPNGAGKSTTFNLVTGVLPATRGA
                     VTLLGRRIDALSSRAIASRGVSRTFQHVKMIPGMTVLENVALGAHLRGSRGVAAAMLR
                     LDRAEERRLLKEAEKQLRRIGMQGSMHELAGNLALGPQRLMEIARALCADPTLLLLDE
                     PAAGLRLKEKQALADVLRQLRGEGIAILLVEHDMDLVMGLVDRIVVMEFGTKLIEGTP
                     EEVQASPAVRAAYLGTEH"
     gene            1413..2153
                     /locus_tag="VNU48_12355"
     CDS             1413..2153
                     /locus_tag="VNU48_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI12115.1"
                     /translation="MSNELLLDVKDLHAGYGRAEVLHGLNLQARKGSVVTVIGPNGAG
                     KSTLLNALMGCLASRGGIDYQGRSVTLATLEERVMAGIALVPETRALFGTMPVEDNLL
                     LGAYRQVRLGRKDSASAMAEVFKLFPRLEERRAQLAGTLSGGERQMLAIGRALMGKPQ
                     LLMLDEPSLGLAPLVVKEIFRIIDSLRATGVTILLVEQNARAALEVADHGYVLEMGEI
                     ALSGPAAELARDPRVIDTYLGAARKSAA"
     gene            2164..2652
                     /locus_tag="VNU48_12360"
     CDS             2164..2652
                     /locus_tag="VNU48_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWI12116.1"
                     /translation="MPGAGEARKDAVDTLDQSRLTHLVGYAASRAAIALRRVFVQNFA
                     PLDLKVVEFSILMLVAANPQVNQKQLGQALDISPPNMAVMLDRLVERGWVERVRSTRD
                     RRAMHIHLTPRGTELVARAEKIAATMENPALRALSAAERALLIELLLKVAGGKGGGGK
                     RG"
     gene            complement(2715..4166)
                     /locus_tag="VNU48_12365"
     CDS             complement(2715..4166)
                     /locus_tag="VNU48_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HWI12117.1"
                     /translation="MDIRNASPDLYNEDLAPAKDRHWGAFSIFNVWTSDVHSLWGYYL
                     AASLFLLCGSFLNFVIAIGAGSLIIFVLMSLMGYAGEKTGVPYPVLARASFGVWGANL
                     PALVRAVVACFWYGAQTAAASGAIVALLTRTDTLLEFHKTTHVLGHSGLELICYVVVW
                     ALQLLIIQNGMETVRKFQDWAGPAVWVAMLVLAIGLCVKAGGFSFDHGIPQAVLLEKT
                     KDAGVAGEPGSFWALMAVGATWVTYFAALYLNFCDFSRYAKNRDAITRGNLWGLPVNL
                     MLFSLVAGVTTIAAFRVYGEVLLHPELISAKFDSWLLAALAALSFAVATLGINVVANF
                     VSPAFDFANVFPRHISFKRGGYIAAGIALVLYPFAPWEGNAASFVNAIGATMGPLLGV
                     LLVDYYLIAKGRVNVEALYQEHGEYRYNGGWNPNALAATIVGAIFSSLLPNLTRVLPA
                     WWGTYGWFFGVAIGGTVYLVLATLRPRQALATA"
     gene            4585..5355
                     /locus_tag="VNU48_12370"
     CDS             4585..5355
                     /locus_tag="VNU48_12370"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013691573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI12118.1"
                     /translation="MKKLLFVLVLATWLPLGASAAEPTKLRIGVEGAYPPFSSIAPDG
                     KIIGFDIDIANALCAEMKAQCALVQQDFDGMIPALQAHKFDLIIASMSATPERLQVVD
                     FSDKYYNTPNRLIAKAGSTIDGSPASFKGKKIGVQRSTINDRFASAKFSGATVVRYTQ
                     QDEVFLDLAAGRIDATLVDAVAGDVGFLKTPAGKGYAFTGPIYDDPAYFGTGVGVALR
                     KGEPTLRERVNAAIKAIRANGTYKKLQDKYFDFDVYGK"
BASE COUNT          919 a         1941 c         1959 g          818 t
ORIGIN      
        1 cctgtactac ctgctcggca acatcgatgc gctcggcaag tacgacggcc tgctcggcgt
       61 gccggccttg cgcttcttcg gcatcgagct gaacaccggg cgcagcttct tctacctgct
      121 gtggacgatc gtcgtgctgg ccgcgctcgc cgcgaacaac ctgctcgact cgcgcgccgg
      181 ccgcgcgctg cgcgccgtca agggcggctc gacgatggcc gaggcgatgg gcgtcgacag
      241 cttccggacc aaggtcaccg cgttcgtgct cgcggcgctg ctggcgagca tctcgggctg
      301 gctgttcgcg catttccagc gcaccgtgaa cccgacgccc ttcggcctga acatgggcat
      361 cgagtacctg ttcatggcgg tgctcggcgg cgtcgctcac gtctggggcg cgctgatggg
      421 cgcgggcgtc gtcgtgatcc tgaaggacca gctgcaggtc tggctgccga agctgctcgg
      481 cggcagcggc aactacgaga tcatcgtctt cggcattctg ctggtgctgg tgctgcagta
      541 cgcgcgcgac ggcttgtggg ccttcatcga agcccgtttg ccacgcgttc aacgcaaggt
      601 cgactggctc gacgcgcccg cgctgcccga gcgcgagcgc ccgccgcacg gcgaggtcgt
      661 gctcgacctg aaggacgcgc gcaaggagtt cggcgggctg gtcgcggtca acgacgtgag
      721 cttccaggtg cgtgccggcg agatcctcgg cctgatcggg ccgaacggcg cgggcaagtc
      781 gacgaccttc aacctcgtca ccggcgtgct gccggccacg cgcggtgcgg tcaccttgct
      841 ggggcgacgc atcgacgcgc tgagctcgcg cgcgatcgcc agccggggcg tctcgcgcac
      901 cttccagcac gtcaagatga tccccggcat gacggtgctc gagaacgtcg ccctcggcgc
      961 gcacctgcgc ggttcgcgcg gcgtggccgc ggcgatgctg cgcctggatc gcgccgagga
     1021 gcgccgcctg ctgaaggaag ccgagaagca gctgcgccgc atcggcatgc agggcagcat
     1081 gcacgagctt gccggcaacc tcgcgctcgg cccgcagcgc ctgatggaga tcgcccgcgc
     1141 gctgtgcgcc gacccgaccc tgctgctgct cgacgagccc gccgcgggcc tgcgcctgaa
     1201 ggagaagcag gcgctggccg acgtgctgcg ccagttgcgc ggcgagggca tcgcgatcct
     1261 gctggtcgag cacgacatgg acctggtcat gggtctggtc gaccgcatcg tcgtgatgga
     1321 gttcggcacc aagctgatcg agggcacccc cgaagaggtg caggccagcc ccgcggtgcg
     1381 cgcggcgtac ctcggcacgg agcactgaag tcatgagcaa cgaactgctg ctggacgtca
     1441 aggacctgca cgccggctac ggtcgcgccg aggtgctgca cggcctgaac ctgcaggcca
     1501 ggaaaggcag cgtcgtcacc gtgatcggcc cgaacggcgc cggcaagtcg acgctgctga
     1561 acgcgctgat gggctgcctg gcatcgcgcg gcggcatcga ctaccagggc cgctcggtca
     1621 cgctggccac gctcgaggag cgcgtgatgg ccggcatcgc gctggtgccc gagacgcgtg
     1681 ccctcttcgg cacgatgccg gtcgaggaca acctgctgct cggcgcctac cgccaggtgc
     1741 gcctgggccg caaggacagc gcgagcgcga tggccgaggt gttcaagctc ttcccgcggc
     1801 tggaggagcg ccgcgcccaa ctcgccggca ccttgtcggg tggcgagcgc cagatgctgg
     1861 cgatcggccg cgcgctgatg ggcaagccgc agctgctgat gctcgacgaa cccagcctgg
     1921 gcctggcgcc gctggtcgtc aaggagatct tccgcatcat cgacagcctg cgagccactg
     1981 gcgtgacgat cctgctggtc gagcagaacg cgcgcgccgc gctcgaggtg gccgaccacg
     2041 gctacgtgct cgagatgggc gagatcgcgc tgtccggccc ggccgccgag ctcgcccgcg
     2101 acccgcgcgt gatcgacacc tacctcggcg cggcgcgcaa gagtgccgcc tgaaggtctg
     2161 accatgcccg gcgccggcga agctcgcaag gacgcggtcg ataccctcga ccagtcgcgc
     2221 ctgacgcacc tcgtcggcta cgccgcgagc cgcgcggcga tcgcgctgcg gcgcgtgttc
     2281 gtgcagaact tcgcgccgct ggacctgaag gtcgtcgagt tctcgatcct gatgctggtg
     2341 gcggccaacc cgcaggtcaa ccagaagcag ctcggccagg cgctggacat ctcgccgccg
     2401 aacatggccg tgatgctcga ccgcctggtc gagcgcggct gggtcgaacg ggtgcgcagc
     2461 acgcgggacc ggcgcgcgat gcacatccat ttgacgccgc ggggcaccga gctggtggcg
     2521 cgcgccgaga agatcgccgc gacgatggag aacccggcgc tgcgcgcgct ctcggcggcc
     2581 gagcgcgcac tgctgatcga gctgctgctg aaggttgccg gcggcaaggg cggtggcggc
     2641 aagcgcggct gagagccggc gacggtgacg acgacgcgct cggcgatctc gccgggccga
     2701 cgaccgccat cggttcaggc cgtcgccagc gcctggcgcg gccgcagcgt cgccagcacg
     2761 aggtagaccg tgccgccgat cgccacgccg aagaaccagc cgtaggtgcc ccaccatgcc
     2821 ggcagcacgc gggtcaggtt cggcagcagg ctcgagaaga tcgcgccgac gatcgtcgcg
     2881 gccagcgcgt tcgggttcca gccgccgttg tagcggtact cgccgtgctc ctggtagagc
     2941 gcctcgacgt tgaccctgcc cttcgcgatc aggtagtagt cgaccagcag cacgcccagc
     3001 agcggcccca tcgtcgcgcc gatcgcgttg acgaagctcg cggcgttgcc ttcccacggt
     3061 gcgaacgggt acagcacgag cgcgatgccg gcggcgatgt agccgccgcg cttgaagctg
     3121 atgtggcgcg ggaacacgtt ggcgaagtcg aatgccggcg agacgaagtt cgccaccacg
     3181 ttgatgccca gcgtcgccac cgcgaagctc agcgccgcca gcgcggcgag cagccagctg
     3241 tcgaacttgg ccgagatcag ctcggggtgc agcagcacct cgccatagac gcggaacgcc
     3301 gcgatcgtgg tcacgccggc caccagcgag aacagcatca ggttcaccgg caggccccac
     3361 aggttgcccc tggtgatcgc gtcgcggttc ttggcgtagc gcgagaagtc gcagaagttc
     3421 aggtacagcg ccgcgaagta ggtgacccag gtcgcgccga ccgccatcag cgcccagaac
     3481 gaacctggct cgccggccac gcccgcgtcc ttggtcttct cgagcagcac cgcctgcggg
     3541 atgccgtggt cgaacgagaa gccgcccgcc ttcacgcaca ggccgatcgc cagcaccagc
     3601 atcgcgaccc agaccgccgg gccggcccag tcctggaact tgcgcaccgt ctccatgccg
     3661 ttctggatga tcagcagctg cagcgcccag accaccacat agcagatcag ctccagcccc
     3721 gaatggccga gcacgtgggt ggtcttgtgg aactcgagca gcgtgtcggt gcgcgtcagc
     3781 agcgcgacga tcgcgcccga tgcggcggcc gtctgcgcgc cgtaccagaa gcaggcgacg
     3841 accgcgcgca ccagcgccgg caggttcgcg ccccagacgc cgaacgacgc acgcgcgagc
     3901 accgggtagg gcacgccggt cttctcgccc gcgtagccca tcaggctcat cagcacgaag
     3961 atgatcagcg agccggcgcc gatcgcgatc acgaagttca ggaagctgcc gcacagcagg
     4021 aacaggctcg ccgcgaggta gtagccccac aggctgtgca cgtccgaggt ccagacgttg
     4081 aagatgctga aggcgcccca gtgccggtcc ttggcgggcg cgaggtcttc gttgtagaga
     4141 tcgggggagg cattgcggat gtccatggat gggtctccgg tgggttgacg cggtgggtcg
     4201 ggcaccgcaa gcaagccctg gcccagctgc ggggcaactt cctcgcccgc gtcgccggcg
     4261 atcaggccgc cgaacatgcc caggcccatc cccgtgccca ggcgcgcgac cgacaggcgc
     4321 agcagcaccg ggtcgctgag cgcgcgcagg acccgatcgg cggcagccag gcccaacgtg
     4381 cgccggtggc ggccgtggcc gaggaagggc gagagaccgt ggtcgtcgac gggttcatgc
     4441 gggtcgggcg atgcacaaac ggcctggatc acaggccggc gcgccgccgc gggaaactgg
     4501 cggtggccgg attgtggcgc ttcgcggcgc cgctacagtg cggcatgcgg cgccatccat
     4561 ccgcgctcgc gcccggagtt gttcatgaag aagttgcttt tcgttctcgt cctggccaca
     4621 tggctgccgc tgggcgcgtc ggccgccgaa ccgacgaagc tgcgcatcgg cgtcgagggc
     4681 gcttacccgc cgttcagctc gatcgccccc gacggcaaga tcatcggctt cgacatcgac
     4741 atcgcgaacg cgctgtgtgc cgagatgaag gcgcagtgcg cgctggtcca gcaggacttc
     4801 gacggcatga tcccggcact gcaggcgcac aagttcgacc tgatcatcgc gtcgatgtcg
     4861 gcgacgccgg aacggctcca ggtggtcgac ttctccgaca agtactacaa cacgccgaac
     4921 cgcctgatcg ccaaggccgg cagcacgatc gacggctcgc ccgcgagctt caagggcaag
     4981 aagatcggcg tgcagcgcag caccatcaac gaccgcttcg cttccgccaa gttcagcggc
     5041 gcgacggtgg tgcgctacac ccagcaggac gaggtcttcc tcgacctcgc cgccggccgc
     5101 atcgacgcga cgctggtcga cgcggtcgcc ggcgacgtcg gcttcctgaa gacgccggcc
     5161 ggcaagggct acgcgttcac cggcccgatc tacgacgacc cggcctactt cggcaccggc
     5221 gtcggcgtcg cgctgcgcaa gggcgagccg acgctgcgcg agcgcgtcaa tgcggcgatc
     5281 aaggcgatcc gcgcgaacgg cacgtacaag aagctgcagg acaagtactt cgacttcgac
     5341 gtctacggca agtaggccgc gcgattgatg ggtgccggag accggcagcg gacctttgca
     5401 atgagataca ccgtttgcag gcaacgcggc gtgcagagtg gcgcgataga aactaaggtt
     5461 ggtctgtggg ctgcgctcgg gaacctgtgt tgccgaaagc ccggcatctc caatcctcga
     5521 tacctgcgag cctctaccat gcacatcact gccgaaacca aagccgccgt tggggcgaaa
     5581 ctcaaggcgt ttcgttctgc aagaggcctc agcgccgaca cggtcgcgcg cgaggcc
//
LOCUS       DATMJB010000581         2510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_383813, whole genome shotgun sequence.
ACCESSION   DATMJB010000581 DATMJB010000000
VERSION     DATMJB010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383813"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /gene="mreB"
                     /locus_tag="VNU48_12375"
     CDS             complement(<1..590)
                     /gene="mreB"
                     /locus_tag="VNU48_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HWI12119.1"
                     /translation="MFGSFRRYFSTDLAIDLGTANTLIYVRGKGIVLDEPSVVAIRHE
                     GGPNGKKTIQAVGAEAKAMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIKMVHPR
                     GILRPSPRIIICVPCGSTQVERRAIRESALGAGASEVFLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYKGSIRVGGDKFD"
     gene            891..1193
                     /gene="gatC"
                     /locus_tag="VNU48_12380"
     CDS             891..1193
                     /gene="gatC"
                     /locus_tag="VNU48_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HWI12120.1"
                     /translation="MALTPQDVSRIAQLARLELQDAERAAMLTQLNGFFAVVEQMSAV
                     DTSGIEPLYTPLSAVQEVTLRLRDDAVSESDQREANQRSAPAVEDGLFLVPKVIGA"
     gene            1273..>2510
                     /gene="gatA"
                     /locus_tag="VNU48_12385"
     CDS             1273..>2510
                     /gene="gatA"
                     /locus_tag="VNU48_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HWI12121.1"
                     /translation="MKAIHHMGVAELGRAMAEKRVSSVEATKHLLARLAAHQQLGAML
                     EVDADHALQQAQDTDARRAGGALGPLLGVPLAHKDIFVTRALPTTAGSKILAGYRSPF
                     DATVVARLAESGMVTLGKLNCDEFAMGSSNENSAYRPVKNPWDPTRVPGGSSGGSAAA
                     VAARLMPAATGTDTGGSIRQPASFSGITGIKPTYGVCSRYGMIAFASSLDQAGPLARS
                     AEDCALLLGAMSGFDERDSTSALRPPQDFHAAMLAPRAGASAAQPLKGLRIGLPKEFF
                     PAALAADVNGAVRAALAEFEKLGATLVDVSLPRTELSIPVYYIIAPAEASSNLSRFDG
                     VRYGHRAAQYSDLEDMYKKSRSEGFGAEVKRRIMIGTYVLSHGYYDAYYLQAQKIRRL
                     IARDFAEAFKQCDVIMGPAAP"
BASE COUNT          398 a          874 c          863 g          375 t
ORIGIN      
        1 tcgtcgaact tgtcgccgcc gacgcggatg ctgcccttgt agaccatgcc gccgaggctg
       61 atgacgccga cctcggtggt gccgccgccg atgtcgacca ccatcgagcc gctcgcctca
      121 gacaccggca ggccggcacc gatcgccgcg gccatcggct cctcgatcag gaagacctcg
      181 ctggcgccgg cgccgagcgc cgattcgcgg atcgcgcggc gctcgacctg cgtcgagccg
      241 cagggcacgc agatgatgat gcgcgggctc ggcctgagga tgccgcgcgg gtggaccatc
      301 ttgatgaact gcttgagcat ctgctcggtg acggtgaagt cggcgatcac gccgtccttc
      361 atcgggcgga tcgcctcgat gttgcccggc accttgccga gcatcgcctt ggcttcggcg
      421 ccgaccgcct ggatcgtctt cttgccgttg gggccgcctt cgtggcggat ggcgacgacc
      481 gagggctcat cgagcacgat gcccttgcca cgcacgtaga tcagcgtgtt ggcggtgccg
      541 aggtcgatgg ccagatcagt cgagaagtaa cgccgaaagg atccgaacat ggtgtgtggg
      601 gcgttgattc gtcacgcggc ctgcgggcaa tgaagcgggc gcggcgccgc tggcaaatcg
      661 gagtgcgttg atgaacgttg ttgttggacg ccgaccgctg cgttgcgcgc ctgcggtgag
      721 ctcatgacac ccgcgctgcg agcgtgcgtc aaatcgcggg tcgatgccga tttgcacagg
      781 ctcgaagggc ttgtggataa ccggggataa taccgcaagc cccggggttt tcgaccggtt
      841 gcgcgccgcg ttggaccgta aaaaccccgc ccgattcctc gattgattcc atggccctga
      901 ctccccaaga cgtgagccgc atcgcgcagc tggcgcgcct cgaactgcag gacgccgaac
      961 gcgccgcgat gctgacccag ctgaacggct tcttcgccgt cgtcgaacag atgagcgcgg
     1021 tcgacacgag cggcatcgag cccttgtaca ccccgctgtc ggcggtgcag gaagtgacgc
     1081 tgcgcctgcg cgacgacgcg gtcagcgaat ccgaccagcg cgaagccaac cagcgcagcg
     1141 cgccggcggt cgaggacggg ctcttcctgg tgccgaaggt catcggcgca tgagccgccg
     1201 cgccgggccg ccccaagcaa ggctcgttcc ctcgggggac cggccgatgt actcatgggc
     1261 cgaggggctc acatgaaggc gattcaccac atgggcgtgg ccgagctcgg ccgcgcgatg
     1321 gccgagaagc gcgtttccag cgtcgaggcg acgaaacatc tgctcgcacg ccttgccgcg
     1381 caccaacaac tcggcgcgat gctcgaggtc gacgcggacc acgcgctgca gcaggcgcaa
     1441 gacaccgacg cgcgccgtgc cggcggcgcg ctcggcccgc tgctgggcgt gccgctcgcg
     1501 cacaaggaca tcttcgtcac gcgggcgctg ccgaccaccg ccggctcgaa gatcctggcc
     1561 ggctatcgca gcccgttcga tgcgacggtg gtcgcgcggc tggccgagtc gggcatggtg
     1621 acgctgggca agctcaactg cgacgagttc gcaatgggct cgagcaacga gaactcggcg
     1681 taccggccgg tcaagaaccc gtgggacccg acgcgcgtgc cgggcggctc gtcgggcggc
     1741 tcggccgccg cggtcgccgc gcggctgatg ccggccgcca ccggcaccga caccggcggc
     1801 tcgatccgcc agccggccag cttcagcggc atcaccggca tcaagccgac ctacggcgtg
     1861 tgttcgcgct acgggatgat cgcgttcgcc tccagcctcg accaggccgg cccgctggca
     1921 cgcagcgccg aagactgcgc gctgctgctc ggcgcgatga gcggcttcga cgaacgcgat
     1981 tcgaccagcg cgctgcgccc gccgcaggac ttccatgccg cgatgctcgc gccgcgcgca
     2041 ggcgccagcg cggcgcagcc cctgaagggc ctgcgcatcg ggctgccgaa ggaattcttc
     2101 ccggccgcgc tggccgccga cgtgaacggc gccgtgcgcg ccgcgctggc cgagttcgag
     2161 aagctcggcg cgacgctggt cgacgtgagc ctgccgcgca ccgagctgtc gattcccgtc
     2221 tactacatca tcgcgccggc cgaggcgagt tcgaacctga gccgcttcga cggcgtgcgc
     2281 tacgggcacc gcgccgcgca gtacagcgac ctcgaggaca tgtacaagaa gagccgcagc
     2341 gaaggcttcg gcgccgaggt caagcgccgc atcatgatcg gcacctacgt gctctcgcac
     2401 ggctactacg acgcctacta cctgcaggcg cagaagatcc gccgcctgat cgcgcgcgac
     2461 ttcgccgagg cgttcaagca gtgcgacgtg atcatggggc ccgccgcgcc
//
LOCUS       DATMJB010000582         2882 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_282119, whole genome shotgun sequence.
ACCESSION   DATMJB010000582 DATMJB010000000
VERSION     DATMJB010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282119"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..1125
                     /gene="bamB"
                     /locus_tag="VNU48_12390"
     CDS             7..1125
                     /gene="bamB"
                     /locus_tag="VNU48_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HWI12122.1"
                     /translation="MVVHVSNRWLAALVGVALLAGCAASKPKPEPLAPLTPKMSASVA
                     WNQHLDGVKFPLAVAVTRGQFTVAGDDGTVLALDAETGREVWRGQAGAKLSAGVGSDG
                     RFAAVVTRDSELVVFEQGQVKWHKPMGGRVTTAPLVAGERVFVLGVDRSVQAFDALDG
                     RKLWVLQRSGDPLTLAQSGVLAAFKDTLIVGQGPRMVGVDPLQGTVRWDVAVGAPRGA
                     NEIERLADLVGPAARIGELVCARSFQAAVGCVNAQSGSSLWSKNIGGIDAIGGDEQFV
                     FAGDATDRISAWKTASGDVAWTSDKLQYRGLSAPLSIGKTVVFGDEDGTIHWFARDTG
                     EAQLRMPTDGSAIVAPPVASGATMLVVTRKGGLFAFRL"
     gene            1148..2482
                     /gene="der"
                     /locus_tag="VNU48_12395"
     CDS             1148..2482
                     /gene="der"
                     /locus_tag="VNU48_12395"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWI12123.1"
                     /translation="MKPVIAIVGRPNVGKSTLFNRMTKSRDAIVADFAGLTRDRHYGD
                     GRSGEHEFIAVDTGGFEPDSTTGIVKEMAKQTRQAVAEADAVIFVVDVRSGVSGQDHD
                     IARYLRTVGKKVILAVNKAEGMQESPLLAEFFELGLGDPHPISAAHGQGIRNLTEAAL
                     AHFEFDDEEEATPEDGGPIRLAVAGRPNVGKSTLINTWLGEERLVAFDQPGTTRDAIT
                     VPFERGGQKFELIDTAGLRRKGKVFEAIEKFSVVKTLQAIADANVVLLLLDATQGVTD
                     QDAHIAGYILESGRAVVIAINKWDAVDDYQKQTLQRSIEQRLAFLKFAAVLHISAIKR
                     QGLGPVWKAIGDAHASAVKKMPTPVLTRLLQEAVEHQAPKRAGAFRPKLRYAHQGGMN
                     PPLIVIHGNSLDHITDSYKRFLEGRFREHFKLTGTPMRIEMRSSKNPFDSKD"
     gene            2583..2828
                     /gene="hfq"
                     /locus_tag="VNU48_12400"
     CDS             2583..2828
                     /gene="hfq"
                     /locus_tag="VNU48_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018902784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HWI12124.1"
                     /translation="MSNKGQLLQDPFLNLLRKEHVPVSIYLVNGIKLQGHIESFDQYV
                     VLLRNTVTQMVYKHAISTVVPGRAVNFHAAEPTDPAA"
BASE COUNT          473 a          970 c         1011 g          428 t
ORIGIN      
        1 ggtcgcctgg tcgtgcacgt ctcgaatcgc tggctcgccg cactggtggg tgtggcgctg
       61 ctggccggct gcgcggccag caagccgaag ccggaaccgc ttgcgccgct gaccccgaag
      121 atgagcgcca gcgtcgcgtg gaaccagcac ctggacggcg tgaagtttcc gctcgcggtg
      181 gcggtgacgc gcggccagtt cacggtcgcc ggcgacgacg gcaccgtgct cgcgcttgat
      241 gccgagaccg gccgcgaggt ctggcgcggc caggccggcg ccaagctcag cgccggcgtc
      301 ggcagcgacg ggcgcttcgc cgcggtcgtg acgcgcgaca gcgagctggt ggtgttcgag
      361 caaggccagg tcaagtggca caagcccatg ggtggccgcg tcaccaccgc gccgctggtg
      421 gccggcgagc gcgtgttcgt gctcggcgtc gaccgctcgg tgcaggcctt cgacgctcta
      481 gacggtcgca agctctgggt cctgcagcgc tcgggcgacc cgctgacgct ggcgcagtcg
      541 ggcgtgctgg ccgcgttcaa ggacaccttg atcgttggtc agggcccgcg catggtcggt
      601 gtcgatccgc tgcaaggcac ggtgcgctgg gacgtcgcgg tgggcgcgcc gcgcggcgcc
      661 aacgagatcg agcgcctggc cgacctggtc gggcctgcgg cgcgcatcgg cgagctggtc
      721 tgcgcgcggt cgttccaggc cgcggtgggt tgcgtcaatg cgcagagcgg cagcagcctg
      781 tggagcaaga acatcggcgg catcgacgcg atcggcggcg acgagcagtt cgtcttcgcc
      841 ggcgacgcga ccgatcgcat cagcgcctgg aagaccgcca gcggcgatgt cgcctggacc
      901 agcgacaagc tgcagtaccg cggcctgagt gcgccgctga gcatcggcaa gacggtggtg
      961 ttcggcgacg aggacggcac gatccattgg ttcgcgcgcg acaccggcga ggcgcagttg
     1021 cgcatgccca ccgacggcag cgcgatcgtc gcgccgccgg tcgcgtcggg cgcgacgatg
     1081 ctggtggtca cgcgcaaggg cgggctgttc gccttccgcc tctgaacgcg cgggagttcg
     1141 ggtcgcgatg aaaccggtga tcgccatcgt cggccgcccc aacgtgggca agtcgacgct
     1201 gttcaaccgc atgacgaaga gccgcgacgc gatcgtcgcc gacttcgccg gcctgacgcg
     1261 cgaccgtcac tacggcgacg gccgcagcgg cgagcacgag ttcatcgccg tcgacaccgg
     1321 cggcttcgag cccgacagca ccaccggcat cgtcaaggag atggccaaac agacgcgtca
     1381 ggccgtcgcc gaggccgacg cggtgatctt cgtcgtcgac gtgcgctcgg gcgtgtcggg
     1441 ccaggaccac gacatcgcac gctacctgcg cacggtcggc aagaaggtga tcctcgcggt
     1501 caacaaggcc gagggcatgc aggagtcgcc gctgctggcc gagttctttg agctcggtct
     1561 gggcgacccg cacccgatct cggcggcgca cgggcagggc atccgcaacc tgaccgaggc
     1621 ggcgctcgcg cacttcgagt tcgacgacga ggaagaggcc acgcccgaag acggcgggcc
     1681 gatccgcttg gccgtcgccg gccggccgaa cgtcggcaag tccacgctga tcaacacctg
     1741 gctcggcgag gagcgcctgg tcgcgttcga ccagccgggc acgacgcgcg acgcgatcac
     1801 ggtgccgttc gagcgcggcg gccagaagtt cgagctgatc gacaccgccg gtctgcgccg
     1861 caagggcaag gtgttcgagg cgatcgagaa gttctcggtc gtgaagacgc tgcaggcgat
     1921 cgccgacgcg aacgtcgtgc tgctgctgct cgacgcgacc cagggcgtga ccgaccagga
     1981 cgcgcacatc gccggctaca tcctggaaag cggccgcgcg gtggtgatcg cgatcaacaa
     2041 gtgggacgcg gtcgacgact accagaagca gacgctgcag cgctcgatcg agcagcgcct
     2101 ggcgttcctg aagttcgcgg cggtgctgca catctcggcg atcaagcgcc agggcctcgg
     2161 cccggtctgg aaggcgatcg gcgacgcgca cgcctcggcg gtcaagaaga tgcccacgcc
     2221 ggtgctgacg cggctgctgc aggaggccgt cgagcaccag gcgcccaagc gcgccggcgc
     2281 gttccggccc aagctgcgct acgcgcatca gggcggcatg aacccgccgc tgatcgtgat
     2341 ccacggcaat tcgctggatc acatcaccga ttcgtacaag cgcttcctcg aggggcgctt
     2401 ccgcgagcac ttcaagctga cgggcacgcc gatgcgcatc gagatgcgct cgtcgaagaa
     2461 cccgttcgac agcaaggatt gaccgtcgcg gccgctccga gggcaaagcc cccaaacttg
     2521 ctgcgccgcg tcatcgctgt gttaacgtgg ccgcttgtcg aactttcatc acggagaata
     2581 tcgtgagcaa caaagggcaa ctcctacaag accccttcct gaacctgctg cgcaaggagc
     2641 acgttccggt ttcgatctac ctcgtcaacg ggatcaagtt gcagggccac atcgagtcgt
     2701 tcgatcagta cgtggtgctg ctgcgcaata ccgtgacgca gatggtctac aaacacgcca
     2761 tctcgaccgt cgtgccgggc cgcgccgtga acttccacgc cgccgagccg accgatccgg
     2821 ccgcgtgagc ttgacaccgc gtcgcttgac cgaccttctc gcgccttgag ttccgcggac
     2881 tc
//
LOCUS       DATMJB010000583         4383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_794989, whole genome shotgun sequence.
ACCESSION   DATMJB010000583 DATMJB010000000
VERSION     DATMJB010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794989"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="VNU48_12405"
     CDS             <1..839
                     /locus_tag="VNU48_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWI12125.1"
                     /translation="GLTLVFGLMDVLNFGHGVFIALGAFVASTVLGGMGDWTGSQEVW
                     RNLVAVFPAMLVAMAVAGAIGLAFERFIVRPVYGQHLKQILITMGGMIIGEELIKVIW
                     GATPITIEMPEALRGVVTLFGDAAVEKFRLLATVVGVLVLVAMLFTLNRTKLGLLIRA
                     GVQDREMVESLGYRIRRLFVGVFVAGSALAGLGGALWGIYQHGVLPQIGAQVNVLIFI
                     VIIIGGLGSTTGCLIGALLVGLMANYTGFLAPKVAMFSNIGLMVAILLWRPQGLYPVA
                     NR"
     gene            850..1926
                     /locus_tag="VNU48_12410"
     CDS             850..1926
                     /locus_tag="VNU48_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWI12126.1"
                     /translation="MLNRLLSHDLPRNRALALVLLVILLALALTPFLFPGTKALNVAA
                     KIIVFILLAASFDLLLGYTGIVSFAQTMFFGIGAYGVAIASSMIGPTWGALAFGIAVA
                     LLVSLALSLLIGLFSLRVKAIFFAMITLAVASAFLTLASQLSDFTGGEDGLSFKVPEA
                     LQPGTEYLNGTLLGASVDGRFVTYYLLFFVAFALVLMLLRIVNSPFGRVLQAIRENDF
                     RAEAIGYRTVVYRTIASVLSALFATLAGVLLALWLRYNGPDTTLSFEIMLDVLLIVVI
                     GGMGTVYGAVIGGVLLVLAQNYLQDLMKLGASTVEGMPLVAQLVSPDRWLLWLGILFV
                     LSVYYFPSGVVGRLRALRRGGAAP"
     gene            1947..2765
                     /locus_tag="VNU48_12415"
     CDS             1947..2765
                     /locus_tag="VNU48_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI12127.1"
                     /translation="MVCEGRELHYTEWGAQHRETVIAWHGLARTGRDMDDLAAHLAQR
                     YRVICPDTIGRGLSQWSPEPEREYCLAFYEKLALALVDQLGLAEFHWIGTSMGGAIGT
                     RAAAGALRGRVRRLILNDNGPELAPAAIARIRGYAGNPQSFATVTELEQYFRTVYKPY
                     GALTDAQWRRLTETSTRRLPDGRVTPHYDPSMVMQFTHHVDDYLQWDDWDRLDIPVLC
                     LRGESSDLLLPETAEQMRRRGPCAVVVEIAGCGHAPALNTPQQWALVERFFGAA"
     gene            complement(2794..3801)
                     /locus_tag="VNU48_12420"
     CDS             complement(2794..3801)
                     /locus_tag="VNU48_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWI12128.1"
                     /translation="MNEPVLHSLAVSQVMLNYAASHGVDAQTCLVGTGIAPEQFQDAE
                     ALIAREQEMQLIENLMLALPDVPALGFELGMQYNVSTFGAWGFALRTSRTLLEAVDRA
                     IRYLPLSTAYCAFSTMVREDELVICADPSAIPAQLRRLLLERDLGTSVNLILELNFAG
                     RSIRRIEFAGQAPSYADRIAGLCPFPVQFNHRHDCIVVSLSDFIKPLPTYDERLVRVL
                     EHQCRQLLDRRQIGGIAGRIRHQLLQATGLLATLDDVAEASNMSTRSLRRKLESEGTS
                     FRLIVEDVRHQLALQLLEATDMKLDEMAIQLGYADTASFTRAFRRWQGVSPGQYRALG
                     T"
     gene            3947..>4383
                     /locus_tag="VNU48_12425"
     CDS             3947..>4383
                     /locus_tag="VNU48_12425"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWI12129.1"
                     /translation="MEHELAALNRQALMAAKPYMGSFAWGTVALAAAMLMAYPATLWL
                     TVTGALPLWAGFAVIATVMYMSYTPVHEAVHGNIHGDRAGLKWVNDLCGYLCGQLTLL
                     PYSTHKVEHFTHHRFTNQAGKDPDCVVSRMGDGPAAFIVTVGR"
BASE COUNT          654 a         1434 c         1495 g          800 t
ORIGIN      
        1 cgggcctgac gctggtgttc ggactgatgg acgtgctgaa cttcggccac ggcgtgttca
       61 tcgcactggg tgccttcgtc gccagcacgg tgctcggcgg catgggcgac tggaccggct
      121 cgcaggaggt ctggcgcaac ctggtcgcgg tgttccccgc gatgctggtg gcgatggcgg
      181 tagccggcgc gatcggcctg gccttcgagc gcttcatcgt gcggccggtc tacggccagc
      241 acctgaagca gatcctgatc acgatgggcg gcatgatcat cggcgaggag ctgatcaagg
      301 tgatctgggg cgcgacgccg atcacgatcg agatgcccga ggcgctgcgc ggcgtcgtca
      361 ccttgttcgg cgacgcggcg gtcgagaagt tccgcctgct cgcgaccgtg gtcggcgtgc
      421 tcgtgctcgt ggcgatgctg ttcaccttga accgcaccaa gctcggcctg ctgatccgcg
      481 ccggcgtgca ggaccgcgag atggtcgaga gcctgggcta ccgcatccgg cggctgttcg
      541 tcggcgtgtt cgtcgccggc tcggcgctcg ccgggctcgg cggtgcgctg tggggcatct
      601 accagcacgg cgtgctgccg cagatcggcg cgcaggtgaa cgtgctgatc ttcatcgtca
      661 tcatcatcgg cggcctcggc tcgaccaccg gctgcctgat cggcgcgctg ctggtcgggc
      721 tgatggcgaa ctacaccggc tttctcgcgc ccaaggtcgc gatgttctcg aacatcgggc
      781 tgatggtggc gatcctgctg tggcggccgc agggcctgta cccggtggcc aaccgttgag
      841 ggcgtcgaga tgctgaaccg actgctgtcg cacgacctgc cgcgcaaccg cgccctcgcg
      901 ctcgtgctgc tggtcattct gctggcgctg gcgctgacgc cgttcctgtt tcccggcacc
      961 aaggcgctga acgtggcggc gaagatcatc gtctttatcc tgctcgcggc gagcttcgac
     1021 ctgctgctgg gctacaccgg catcgtcagc ttcgcgcaga cgatgttctt cggcatcggc
     1081 gcctacggcg tggcgatcgc cagcagcatg atcgggccga cctggggcgc gctcgcgttc
     1141 ggcatcgcgg tcgcgctgct cgtgtcgctc gcgctgtcgc tgctgatcgg cctgttcagc
     1201 ctgcgcgtga aggcgatctt cttcgcgatg atcacgctgg cggtggcctc ggcctttctg
     1261 accctggcct cgcagctgtc ggacttcacc ggcggcgaag acggcctgag cttcaaggtg
     1321 cccgaggcgc tgcagcccgg caccgagtac ctgaacggca cgctgctcgg cgcgtccgtc
     1381 gacggccgct tcgtcaccta ctacctgctg ttcttcgtcg cgttcgcgct ggtgctgatg
     1441 ctgctgcgca tcgtcaactc gccgttcggg cgcgtgctgc aggcgatccg cgagaacgac
     1501 ttccgcgccg aggcgatcgg ctaccgcacc gtcgtctacc gcaccatcgc gtcggtgctg
     1561 tcggcgctgt tcgcgacgct ggccggcgtg ctgctcgcgc tctggctgcg ctacaacggg
     1621 ccggacacga cgctgtcgtt cgagatcatg ctcgacgtgc tgctgatcgt cgtgatcggt
     1681 ggcatgggca cggtctacgg cgcggtcatc ggcggcgtgc tgctggtgct ggcgcagaac
     1741 tatctgcagg acctgatgaa gctcggcgcg tcgacggtcg agggcatgcc gctcgtcgcg
     1801 caactcgtct cgccggaccg ctggctgctg tggctgggca tcctgtttgt gctgtcggtc
     1861 tactactttc cgagcggcgt ggtcggcagg ttgcgggcgc tgcgccgcgg gggggccgcg
     1921 ccgtgagctt cttcagctcg aactacatgg tctgcgaagg ccgcgaactg cactacaccg
     1981 aatggggtgc gcagcaccgc gagaccgtga tcgcctggca cggcctggca cgcaccgggc
     2041 gcgacatgga cgacctcgcc gcgcacctcg cgcaacgcta ccgcgtgatc tgccccgaca
     2101 cgatcggccg cggcctgagc cagtggagtc ccgagcccga gcgcgaatac tgcctggcgt
     2161 tctacgagaa gctcgcgctc gcgctggtcg accagctcgg cctggccgag ttccactgga
     2221 tcggcacctc gatgggcggc gcgatcggca cgcgcgcggc ggccggcgcc ttgcgcgggc
     2281 gcgtgcggcg cctgatcctg aacgacaacg gacccgagct cgcgccggcc gcgatcgcgc
     2341 gcatccgcgg ctacgccggc aacccgcaat cgttcgcgac cgtgaccgaa ctcgagcagt
     2401 acttccgcac cgtctacaag ccctatggcg cgctgaccga cgcgcaatgg cggcgcctga
     2461 ccgaaacctc gacacgccgc ctgcccgacg gccgcgtgac gccgcactac gacccgtcga
     2521 tggtgatgca gttcactcac cacgtcgacg actacctgca gtgggacgac tgggatcgcc
     2581 tggacattcc ggtgctgtgc ctgcgcggcg agagctccga cctgctgctg cccgagacgg
     2641 ccgagcagat gcgccggcgc gggccctgtg cggtggtcgt cgagatcgcc ggctgcggcc
     2701 atgcgccggc gctgaacacg ccgcagcagt gggcgctggt ggagcggttc ttcggggcgg
     2761 cctgagagcg cgcgggcaaa gcgaacgttc gaatcaagtg cctagcgcgc ggtactggcc
     2821 aggggacacc ccctgccatc gtcggaacgc gcgcgtgaag ctggcggtgt ctgcgtagcc
     2881 gagctggatg gccatctcgt ccagcttcat gtcggttgct tcgagcagct gcagcgccaa
     2941 ctgatggcgg acatcctcaa cgatgagccg aaagctggtg ccctctgatt ccagtttgcg
     3001 acgcaggctg cgcgtcgaca tgttggacgc ttcggcaaca tcgtcaagcg ttgccaacag
     3061 acccgtggct tgcagcagct ggtgcctgat gcgtccggcg atgccgccga tctggcgccg
     3121 atccagcagc tgccgacact gatgttccag gactcgcacg agtcgctcgt cgtacgtcgg
     3181 caggggtttg atgaagtcgc tgaggctgac gacgatgcaa tcgtgtcgat gattgaattg
     3241 aaccgggaat gggcagagcc cggcgatgcg atcggcgtag gacggcgcct ggccggcgaa
     3301 ctcgatgcgc cggatcgagc gcccggcgaa attgagttcc aggatcagat tgacggacgt
     3361 ccccaggtca cgttccagca gcaaccggcg cagctgtgcc gggatggcgg agggatccgc
     3421 gcagatgacg agctcgtctt cgcgcaccat cgtggagaag gcgcagtacg cagtgctgag
     3481 cggcaggtat cggatcgcgc gatccaccgc ttccagcaag gtcctgctcg tgcgcagggc
     3541 gaatccccag gcgccgaagg tggagacgtt gtattgcatg ccgagctcga aaccgagagc
     3601 gggcacgtcg ggcaaggcca gcatcaggtt ctcgatcagc tgcatttcct gctcgcgcgc
     3661 aatgagggcc tcggcatcct ggaactgctc cggtgcgatg ccggtgccga caagacacgt
     3721 ctgggcatcg acgccgtgag atgcggcgta attgagcatc acctggctga cggccaggct
     3781 gtgcaggact ggctcgttca ccatccattc cccttcggtg ctccgcagat tctgcgtcag
     3841 cgctcgcggc tggccacaaa tgcatggtga aaggccacga ctgcgctgtc ggcggcaccc
     3901 catgagccct aagctttgct ccgtcgcctt cgaaaggacc tcgggaatgg agcacgaact
     3961 cgctgcgctg aaccggcagg ccctcatggc cgccaagccg tacatgggca gtttcgcatg
     4021 gggcacggtg gcgctcgccg cggccatgct gatggcttac ccggccaccc tgtggctgac
     4081 ggtcaccggg gccttgccgc tgtgggccgg attcgcagtg atcgccaccg tcatgtacat
     4141 gtcgtacacg cctgtgcacg aggcggtgca cggcaatatc catggcgatc gcgctggtct
     4201 caaatgggtc aacgatcttt gcggctatct ttgcgggcag ctgaccctcc tgccgtactc
     4261 gacgcacaag gtcgagcact tcacgcatca tcgttttacc aatcaggccg gcaaggatcc
     4321 ggactgcgtg gtcagccgga tgggcgacgg accggcggcc ttcatcgtga ccgtcggcag
     4381 gat
//
LOCUS       DATMJB010000584         2553 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_380022, whole genome shotgun sequence.
ACCESSION   DATMJB010000584 DATMJB010000000
VERSION     DATMJB010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380022"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VNU48_12430"
     CDS             complement(<1..728)
                     /locus_tag="VNU48_12430"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HWI12130.1"
                     /translation="MSLIPPATTPSDATAAPAPLVGPASDRATSRWARLRWVARALLG
                     LVGIVWSLLLIAWLTLHWGILPHIQQWREPIEQRASRALGVPLRIGEIVVRSSGWVPS
                     FELREVLLLDANQQPALRLPRVFAALSPRSLLSLDLRFEQLLIDGPQLEVRRDKTGRI
                     FVAGLDFSGAEVGGGSAAADWFFAQREFVVRGGALRWTDELRGADPLALSDVQLVVRN
                     GLREHALRLDATPPPEWGERFSAVG"
     gene            860..>2553
                     /locus_tag="VNU48_12435"
     CDS             860..>2553
                     /locus_tag="VNU48_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-synthetase adenylyltransferase"
                     /protein_id="HWI12131.1"
                     /translation="MGSRPAPIVTDSRLVAPAAAGPSARAGHSRFVQRIRRRYAAELP
                     LLAPGLPTRAHIDALIERLLADGRDTASAMRVARQLVLERLAVLDVEQGAAMSDVTRA
                     MTELAESTLDRALALAQLSHDRRYGAPRNAAGERIEFWVVGMGKLGARELNVSSDIDL
                     VYVYEEDGETDGGDDGAQRISAHEYFSQVCKSLYTLIGETTEDGFVFRVDLALRPNGN
                     SGPPCVSLAMLEEYLQVQGREWERFAWLKSRVVAPGVSVKSGRALALRGLVTSFVYRR
                     YLDYGVFEGLRQLHAKVREEAQRRAAGRPERANDVKLSRGGIREIEFIVQLLLVARGG
                     QSPEIRTRSTLKALGKLAADGLMKPASATRLAEAYEFLRRIEHRIQYLDDQQTHLLPT
                     IDADLAWIARSLGFGCRVGGANNDTATCDLLDLLGATREFVAMEFDALLHDGRAPVAK
                     PGGCRTGNCGVAPPPIDSEAFLEQLPAELAARVQSWREQPRIKMLRDDSKLRLARLIK
                     RAAHAVRDCECTMDAAIRFVDWVEPLLRRESYLAMLVERPEVQTRLLRLLGLARWP"
BASE COUNT          388 a          932 c          909 g          324 t
ORIGIN      
        1 ccgaccgcgc tgaagcgctc gccccattcg ggcggcggcg tcgcgtcgag ccgcagcgcg
       61 tgctcgcgca ggccgttgcg cacgaccagc tgcacgtcgc tcagtgccag cgggtcggcg
      121 ccgcgcagct cgtcggtcca gcgcagcgcg ccaccgcgca ccacgaactc gcgctgcgcg
      181 aagaaccagt cggcggctgc actgccgccg ccgacctcgg ccccgctgaa gtcgagcccg
      241 gcgacgaaga tgcggccggt cttgtcgcgc cgcacctcga gctgggggcc gtcgatcagc
      301 agctgctcga agcgcaggtc cagcgacagc agcgagcgcg gcgacagcgc cgcgaacacg
      361 cgcggcaggc gcagcgcggg ctgctggttc gcgtcgagca gcagcacctc gcgcagctcg
      421 aacgacggca cccagccgct ggagcgaacc acgatctcgc cgatgcgcag cggcaccccg
      481 agcgcgcggc tggcacgctg ctcgatcggc tcacgccatt gctggatgtg gggcagaatc
      541 ccccaatgaa gagtcagcca cgcgatcagc aataagctcc acacgatgcc gaccaggccg
      601 agcaacgcac gcgcgaccca ccgcaggcgc gcccagcgcg atgtggctcg atccgaggcc
      661 ggcccaacaa gtggcgcggg cgcagcggtc gcgtcggagg gtgtcgtggc gggcgggatc
      721 aaggacatgg aaacatcggt gcgccggcca caggttgggc atttcggaca tcggcggcgg
      781 gcgcatcggc atgccgcccg cgaggccgtc ggcactcgcc gctgacgggt caatctagca
      841 cagagcctcg ccgcaccgca tgggttcccg ccccgccccc atcgtcaccg attcccggct
      901 ggtcgcacct gcggccgcag ggccgagcgc gcgcgccggg cacagccgat tcgtgcagcg
      961 catccggcgc cgctatgcgg ccgaactgcc gctgctcgcg cccgggttgc cgacgcgcgc
     1021 gcacatcgac gcgctgatcg agcgcctgct cgccgacggc cgcgacaccg ccagcgcgat
     1081 gcgcgtggcg cgccagctcg tgctggagcg gctcgccgtg ctcgacgtcg aacagggcgc
     1141 tgcgatgagc gacgtgaccc gcgcgatgac cgagctcgcc gagagcacgc tcgaccgggc
     1201 gctggcgctg gcgcagctca gccacgaccg gcgctacggc gcgccgcgca atgccgccgg
     1261 cgaacgcatc gagttctggg tcgtcggcat gggcaagctc ggggcacgcg agctgaatgt
     1321 gtcgtccgac atcgacctgg tctacgtcta cgaggaagac ggcgagaccg atggcggcga
     1381 cgacggcgcc cagcgcatca gcgcgcacga gtatttctcg caggtctgca agagtctgta
     1441 cacgctgatc ggcgagacca ccgaggacgg cttcgtgttc cgcgtcgacc tggcgctgcg
     1501 cccgaacggc aactcgggcc cgccctgcgt gagcctggcg atgctcgagg aatacctgca
     1561 ggtgcagggc cgcgagtggg aacgcttcgc ctggctgaag agccgcgtcg tcgcgcccgg
     1621 cgtgagcgtg aagagcggcc gcgcgctggc gctgcgcggc ctcgtgacct cgttcgtgta
     1681 ccgccgctac ctcgactacg gcgtcttcga gggcctgcgt cagctgcacg cgaaggtgcg
     1741 cgaggaagcg cagcgccgcg ccgccggccg cccggagcgc gccaacgacg tcaagctctc
     1801 gcgcggcgga atccgcgaga tcgagttcat cgtgcagctg ctgctggtgg cgcgcggcgg
     1861 ccagtccccc gagatccgca cgcgttcgac cttgaaggcg ctgggcaagc tcgccgccga
     1921 cgggctgatg aagccggcga gcgcgacgcg cctggccgag gcctacgaat tcctgcgccg
     1981 catcgagcac cgcatccagt acctggacga ccagcagacg cacctgctgc cgaccatcga
     2041 cgctgacctg gcctggatcg cccgcagcct gggcttcggc tgccgcgtcg gcggcgcgaa
     2101 caacgacacc gcgacctgcg acctgctcga cctgctcggc gcgacgcgcg agttcgtcgc
     2161 gatggagttc gacgcgctgc tgcacgacgg ccgcgccccg gtcgcgaagc cgggcggctg
     2221 ccgcaccggc aactgcggtg tggcgccgcc gccgatcgac agcgaggcct tcctcgagca
     2281 gttgccggcc gaactcgccg cccgcgtgca atcctggcgc gagcagccgc gcatcaagat
     2341 gctgcgcgac gacagcaagc tgcggctggc gcggctgatc aagcgcgccg cgcacgcggt
     2401 gcgcgactgc gaatgcacga tggacgcggc gatccgtttc gtcgactggg tcgagccgct
     2461 gctgcggcgc gagagctacc tcgcgatgct ggtcgagcga cccgaggtgc agacccggct
     2521 gctgcgcctg ctgggcctcg cgcgctggcc gat
//
LOCUS       DATMJB010000585         2384 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_436098, whole genome shotgun sequence.
ACCESSION   DATMJB010000585 DATMJB010000000
VERSION     DATMJB010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436098"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VNU48_12440"
     CDS             <1..1266
                     /locus_tag="VNU48_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HWI12132.1"
                     /translation="VVFMAIYMPHASLDTVTQNVYEEMASITLLSIPLFILKGAAIGK
                     SRAGQDLYSALHAWLHRVPGGLGVANVFACALFAAMAGSSPATCSAIGSAGIPEMRKR
                     GYSPGFAAGIIAAGGTLGILLPPSITMILYAVAAEQSLGRLFLAGIGPGLLLVGLFAL
                     YAVLNYRKEYRRARLAFERDGTASALLNDEHFSLREKFEMLPRVVPFVLLLIGVMVAL
                     YGGFATPSETAGLGGLMALVLIALIYGVWRVRDVAPILAATLKESTMLMFIIGMSLLF
                     SYVMSYLHISQSTAQWIVALQLSKWVLLAAILLMVIVLGFFLPPVSIILMTAPIFLPP
                     LKAAGFDLIWFGVLMTIVMETGLIHPPVGLNIFVIKNIAPDIALADIIWGVLPFVVLM
                     LLAVLLICVFPGIATALPDLVMGPVPGKP"
     gene            1422..1667
                     /locus_tag="VNU48_12445"
     CDS             1422..1667
                     /locus_tag="VNU48_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12133.1"
                     /translation="MSQGGSSIDVLSVLRGLLPATAADGVDLNLALPWPGPPPAGPAP
                     ADASGWERSSLELLTGVEVTDFSETISGEVFFELFRR"
     gene            complement(1683..1901)
                     /locus_tag="VNU48_12450"
     CDS             complement(1683..1901)
                     /locus_tag="VNU48_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12134.1"
                     /translation="MPKQATTRKPIPSERRSGDDRRRIEGTPPGKHDRRRGLEPRLPE
                     VVELDMTNSEWMSLSDDPVPPSTVPPRR"
     gene            complement(2022..>2384)
                     /locus_tag="VNU48_12455"
     CDS             complement(2022..>2384)
                     /locus_tag="VNU48_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HWI12135.1"
                     /translation="EAAGVIRGYTALVDPERVGLHLTVMVEVNLTHHSEELVQRFEQA
                     VAASPHIARCQSTTGQADYLMTVLAPDIKHYERLLHETIFKLPGVTHVRSSIVLKEVK
                     SEIALPIGQLLAPPTPRS"
BASE COUNT          315 a          787 c          824 g          458 t
ORIGIN      
        1 gtcgtgttca tggcaatcta catgccgcac gcctcgctcg atacggtgac acagaacgtc
       61 tacgaggaaa tggcctcgat caccctgctg tcgatcccgc tgttcatcct caagggggct
      121 gcgatcggca aatcccgcgc cggacaggat ctctattcgg cattgcatgc ctggctgcat
      181 cgggtgcccg gcgggctcgg ggttgccaac gtgttcgcct gcgcgctgtt cgccgcgatg
      241 gcgggctcga gcccggccac ctgctcggcg atcggcagcg ccggcatccc ggagatgcgc
      301 aagcgcggct actcgccggg cttcgcggcc ggcatcatcg ccgccggcgg cacgctgggc
      361 atcctgctgc cgccgtcgat cacgatgatc ctgtatgcgg tcgcggccga gcagtcgctc
      421 ggccggctgt tcctggccgg catcggcccg gggctgctgc tggtcggctt gttcgccttg
      481 tacgcggtgc tcaactaccg caaggagtac cggcgcgccc ggcttgcctt cgagcgcgac
      541 ggcacggcgt cggcgctgct gaacgacgag cacttcagcc tgcgcgagaa gttcgagatg
      601 ctgccgcgcg tcgtgccctt cgtgctgctg ctgatcggcg tgatggtggc gttgtacggc
      661 ggcttcgcga cgccctcgga aaccgccggc ctgggcgggc tgatggcgct ggtgctgatc
      721 gcgctgatct acggcgtctg gcgcgtccgg gacgtcgcgc cgatcctcgc ggcgacgctg
      781 aaggagtcga cgatgctgat gttcatcatc ggcatgtcgc tgctgttctc gtacgtgatg
      841 agctacctgc acatcagcca gtcgacggcg cagtggatcg tcgcgctgca gctgtcgaag
      901 tgggtgctgc tggcggcgat cctgctgatg gtgatcgtgc tcggcttctt cctgccgccg
      961 gtcagcatca tcctgatgac ggcgccgatc ttcctgccgc cgctcaaggc cgcgggcttc
     1021 gacctgatct ggttcggcgt gctgatgacg atcgtgatgg aaaccggcct gatccacccg
     1081 ccggtcggcc tgaacatctt cgtgatcaag aacatcgcgc ccgacatcgc gctggccgac
     1141 atcatctggg gcgtgctgcc ctttgtcgtg ctgatgctgc tggcggtgct gctgatctgc
     1201 gtcttccccg gcatcgccac cgcgttgccc gatctggtga tggggccggt gcccggcaag
     1261 ccgtgatcgc ccgccccggg cggagcccgg cgctgcaggc ccagtccttg taacgaaatg
     1321 agtcttcccc ctatacttag ggggatgcgc tcaacgccct ggcgaactct cgatggtttc
     1381 tcgccggccc gcgcagccag cgcactccgg ggtgagtttc aatgagtcaa ggcgggtcat
     1441 cgatcgacgt cctgagcgtg ctgcgcggcc tgctgcccgc cacggccgcc gatggcgtcg
     1501 acctgaacct ggcgctgccg tggcccggcc cgccgcccgc cggtccggcg ccggccgatg
     1561 ccagcggctg ggaacgctcg tcgctcgaac tgctgaccgg cgtcgaggtg accgacttct
     1621 cggaaacgat ctcgggcgag gtcttcttcg agctgtttcg gcgctgacga acccgggccc
     1681 gcctatcggc gcggcggaac ggtcgacggc ggcacggggt catcgctcag gctcatccat
     1741 tccgagttcg tcatgtcgag ctccacgact tcgggcaggc gcggctcgag cccgcggcgt
     1801 cggtcatgct tgccgggcgg cgtgccctcg atgcggcggc gatcgtcgcc ggagcgtcgt
     1861 tcggagggga tcggttttcg agttgtcgct tgcttgggca tggctgggaa cgtgtgggaa
     1921 gcttgctgcc atggcgggcc gccggtgtca ggcccgaggc taacaaactt cgcgcgcggc
     1981 gggcgcgagg gccgcgtccg tgagtgtgaa cagtggcccg tctatgaccg cggcgtgggc
     2041 ggcgcgagca gctgcccgat cggcagcgcg atttcggact tgacctcctt cagcacgatg
     2101 ctcgagcgca catgcgtcac gccgggcagc ttgaagatcg tctcgtgcaa caggcgctcg
     2161 taatgcttga tgtcgggcgc cagcaccgtc atcaggtagt cggcctggcc ggtggtggac
     2221 tggcagcgcg cgatgtgcgg gctcgccgcg acggcctgct cgaagcgctg gaccaattct
     2281 tcgctgtgat gagtcaggtt cacctcgacc atcacggtca ggtgcaggcc gaccctctcc
     2341 gggtcgacga gtgcggtgta gccgcggatc acgccggccg cctc
//
LOCUS       DATMJB010000586         2322 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_83792, whole genome shotgun sequence.
ACCESSION   DATMJB010000586 DATMJB010000000
VERSION     DATMJB010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83792"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1817)
                     /gene="kdpA"
                     /locus_tag="VNU48_12460"
     CDS             complement(9..1817)
                     /gene="kdpA"
                     /locus_tag="VNU48_12460"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWI12136.1"
                     /translation="MSSQAWIQLVVFLGVLAVLAWPMGRWLAAVADGHVPRWLAPVRS
                     VENGLYRLAGVDAGAGMGCKQYALGVLAFNVLGVFAVYALQRLQGVLPMNPQGMSAVS
                     PDSSFNTAISFVTNTNWQGYGGEATMSYLTQMLALTVQNFFSAATGIAVVFALIRGFV
                     ARSAGTIGNVWTDLTRITLYVLLPLSLAFAVFLVGQGAIQNFDAYKEVTTLEVNAYQA
                     PKNGPDGQPLKDDKGNPLTEDAKTPTQQIAMGPVASQEAIKMIGTNGGGFFNANSAHP
                     YENPTPLSNFLQMLAIFLIPAGLVFAFGRMVGDMRQGWAVLAAMTLLFVVAVLLVTPP
                     EQAGSPQFTALGVDQAQSALQPGGNMEGKEARFGISATTLFAAITTAASCGAVNGMHD
                     SFMPLGGAVPMMLIQLGEVVFGGVGSGLYGMLIFAILAVFIAGLMIGRTPEYLGKKIE
                     AFDMKMTSIAILVTPILVLAGTALAVSFEAGKAGIANPGAHGFSEILYALSSAANNNG
                     SAFAGLSANTPFWNSLLAVAMWFGRFAVIVPVLAIAGSLAAKKRLAVTPGTLPTHGPL
                     FVVLLIGSVLLVGLLNYVPALALGPVVEHFMLWSGQ"
     gene            complement(1817..1906)
                     /gene="kdpF"
                     /locus_tag="VNU48_12465"
     CDS             complement(1817..1906)
                     /gene="kdpF"
                     /locus_tag="VNU48_12465"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HWI12137.1"
                     /translation="MTWLYWLSGAASSGLLVYLVYALFNAEEF"
     gene            complement(2113..>2322)
                     /locus_tag="VNU48_12470"
     CDS             complement(2113..>2322)
                     /locus_tag="VNU48_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin"
                     /protein_id="HWI12138.1"
                     /translation="LSCACTTCHVVVRQGLASLGEMDEAEEDLLDRAWGLEPNSRLSC
                     QAILSNQDVTIEIPKYTINHAKEEH"
BASE COUNT          421 a          798 c          761 g          342 t
ORIGIN      
        1 gcgcgcggtc actggcccga ccacagcatg aagtgctcga ccaccgggcc cagggcgagc
       61 gccggcacgt agttcagcag gccgaccagc agcaccgaac cgatcagcag cacaacgaac
      121 aacgggccgt gcgtcggcag cgtccccggg gtcaccgcga gacgcttctt ggccgccagg
      181 ctgccggcga tcgcgagcac cggcacgatc accgcgaagc gcccgaacca catcgccacc
      241 gcaagcagcg agttccagaa cggcgtgttc gccgacaggc ccgcgaaggc gctgccattg
      301 ttgttggccg ccgagctcag cgcgtacagg atctcggaga agccgtgcgc gccggggttc
      361 gcgatgccgg ccttgccggc ttcgaacgac accgcgagcg cagtgccggc gagcaccagg
      421 atcggcgtga ccaggatcgc gatcgaggtc atcttcatgt cgaaggcctc gatcttcttg
      481 cccaggtatt caggcgtgcg cccgatcatc agcccggcga tgaagaccgc gaggatcgcg
      541 aagatcagca tgccgtacag cccggagccg acgccgccga acacgacctc gccgagctgg
      601 atcagcatca tcggcaccgc gccgcccagc ggcatgaacg agtcgtgcat gccgttgacc
      661 gcgccgcacg aggcggcggt cgtgatcgcg gcaaacagcg tggtcgcgct gatgccgaag
      721 cgtgcttcct tgccttccat gttgccgccc ggctgcagcg ccgactgggc ctggtcgacg
      781 ccgagggccg tgaactgcgg actgccggcc tgctcgggcg gcgtcacgag gagcaccgcg
      841 acgacgaaca gcagcgtcat cgccgccagc accgcccagc cctggcgcat gtcgccgacc
      901 atgcggccga acgcgaacac caggccggcc gggatcagga agatcgccag catctgcagg
      961 aagttcgaca gcggcgtcgg gttctcgtac gggtgcgccg agttcgcgtt gaagaagccg
     1021 ccaccgttgg tgccgatcat cttgatcgcc tcctgcgacg cgaccggacc catcgcgatc
     1081 tgctgcgtcg gcgtcttcgc gtcctccgtc agcgggttgc ccttgtcgtc cttcagcggc
     1141 tggccgtccg ggccgttctt cggcgcctgg taggcgttga cttccagcgt cgtcacctcc
     1201 ttgtaggcgt cgaagttctg gatcgcgccc tgcccgacca ggaacaccgc aaacgccagc
     1261 gacagcggca gcagcacgta caaggtgatg cgcgtcaggt cggtccacac gttgccgatc
     1321 gtgccggccg agcgcgcgac aaagccgcgg atcagcgcga agaccaccgc gatgccggtc
     1381 gccgcggaga agaagttctg caccgtcagc gcgagcatct gcgtgaggta actcatcgtc
     1441 gcttcgccgc cgtagccttg ccagttggtg ttggtgacga agctgatcgc ggtgttgaac
     1501 gacgagtcgg ggctgaccgc gctcatcccc tgcgggttca tcggcagcac gccctgcagg
     1561 cgctgcagcg cgtacacggc gaacacgccg agcacgttga acgcgagcac gccgagcgcg
     1621 tactgcttgc agcccatgcc ggcgccggcg tcgacgccgg ccaggcggta caggccgttc
     1681 tcgacgctgc gaaccggcgc gagccagcgc ggcacatggc catcggccac cgcggccagc
     1741 cagcgcccca tcggccaggc cagcacggcc agcacaccga ggaagacgac gagctggatc
     1801 caggcctggg agctcatcag aactcctcgg cgttgaacaa cgcgtagacg aggtagacaa
     1861 gcagaccgct cgatgccgcg ccgctgagcc aatagagcca ggtcatttgc gcgctcccag
     1921 cttgtcgcag gcggcaatca gaccgaacag ggccagcgcc agcgcggcgg cagcgcccag
     1981 gtacacgaag tccatacatc cctcacaagg cgttctacaa gcctcgaatg gtggggatgc
     2041 ccgcgtcaaa tcggcgtaga gaccgcggct ggtggcatca agaaagcatc aagaacgcgg
     2101 cgcgaacccg gctcagtgct cttccttggc gtggttgatc gtgtatttcg ggatctcgat
     2161 cgtcacgtcc tgattcgaca ggatcgcctg gcacgacagg cgcgagttcg gctccagacc
     2221 ccacgcgcgg tcgagcaggt cttcttcggc ttcgtccatc tcgccgagcg acgcgagacc
     2281 ttgccgaacc acgacgtggc aggtggtgca ggcgcagctc ag
//
LOCUS       DATMJB010000587         3801 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_473004, whole genome shotgun sequence.
ACCESSION   DATMJB010000587 DATMJB010000000
VERSION     DATMJB010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473004"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..511
                     /locus_tag="VNU48_12475"
     CDS             20..511
                     /locus_tag="VNU48_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HWI12139.1"
                     /translation="MKTLLIVYHTMTGGTLQMARAAAAGAAAETAVQVRLLRAPQAEA
                     ADVLGADGYVFATPENLAAMSGLMKDFFDRCYYGVLDRIEGRPYASLVCAGSDGHNAV
                     RQIERIATGWRLKAVAPALIVCTHAQTPEAILRPKQIDAPDLQRCEELGAALASGLAL
                     GVF"
     gene            642..1277
                     /locus_tag="VNU48_12480"
     CDS             642..1277
                     /locus_tag="VNU48_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HWI12140.1"
                     /translation="MPARPRTPAGAGVKRAAPEAPDNRRADLVRVAARLFREKGFDGT
                     TIRDIAHAVGMRSGSPFYHFANKHELLMAVMEEGLRLGLERTQQVLADATLPAAERFA
                     RLVRTQYGILHDTGSDFIPVMLYDWRSLPAQYKRRIVELKDRYDAIWQRTLDELHAQG
                     LLRADPRLARLMILGAINFSATWYRAKPRAAARVDLDELAAQTVALVLHRR"
     gene            1281..2066
                     /locus_tag="VNU48_12485"
     CDS             1281..2066
                     /locus_tag="VNU48_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010094431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HWI12141.1"
                     /translation="MDNTLLIERDGPVWTVTLNRPAVRNAVDGPTAHALARAFRDFDA
                     DGEACVAVLTGAGGQFCAGADLKAVASGHGGNPLDADLRSDGPMGPTRLQLGKPVIAA
                     VEGHAVAGGLELALWCDLRVASRTAVFGVFCRRFGVPLIDGGTVRLPRLIGESRALDL
                     ILTGRAVAADEALALGLANRVVPAGEALAVARELARQIAAFPQQCLRHDRASVMAQHG
                     APLRDALAHEFALGLRTLQSGESVAGAQRFSDGAGRHGTFSGG"
     gene            complement(2134..3135)
                     /locus_tag="VNU48_12490"
     CDS             complement(2134..3135)
                     /locus_tag="VNU48_12490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWI12142.1"
                     /translation="MKAALIDRYGSNDLVRVAEIAVPEIGAMDLLVQVHAASVNPLDA
                     KTRDGKLKLLLKYRFPLVLGNDLSGVVVDVGERVTRFKKGDAVYARLDKSRIGAFAEF
                     AVVREGAAAPKPSNVTFEEAASLPLVALTAWQALVELGRLGVGQRALIHAGSGGVGSV
                     AIQLAHHLGATVFTTVGQRNLELVKRLGADVPIDYVSKRFEDVARDCDVVLDSAGGDT
                     LVRCFECVKPGGVVVSIGGTPSAAFARSWGLNEFIVLAIRFMSRKAMAAARRRNARYE
                     YLFMRADGEQLREITRLVESGAIKPLVERVFPLEQVRDALAYSESGRATGKVVIKVV"
     gene            3350..>3801
                     /locus_tag="VNU48_12495"
     CDS             3350..>3801
                     /locus_tag="VNU48_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HWI12143.1"
                     /translation="MDLGHIALDSHAAYRVITARDARFDGRLFVGVTSTGIYCRPICR
                     VRTPRERNCRFFATPAQAEAAAFRPCMKCRPEIAPGAPHAWTVMDASRTLARQAADAL
                     DAQAASGDAASLVLVAGRLGVSDRHLRRIFQAEHGVTPLQYLQTRRLLL"
BASE COUNT          582 a         1379 c         1280 g          560 t
ORIGIN      
        1 aggtcggctt cacgctcgag tgaagacgct gctgatcgtc tatcacacga tgaccggcgg
       61 cacgctgcag atggcacgcg ccgccgcggc cggcgctgcg gcggagacgg cggtgcaggt
      121 caggctgctg cgcgcaccgc aggccgaggc cgccgatgtg ctgggtgccg acggctacgt
      181 gttcgcgaca cccgagaacc tggccgcgat gtccgggctg atgaaggact tcttcgatcg
      241 ctgctactac ggcgtgctcg atcgcatcga aggccgcccg tacgcgagcc tggtctgcgc
      301 cggcagcgat gggcacaacg cggtgcgcca gatcgagcgc atcgcgaccg gctggcggct
      361 gaaggccgtc gcgccggcgc tgatcgtctg cacccacgcg cagacgcccg aggcgatcct
      421 gcggccgaag cagatcgacg cgccggacct gcaacgctgc gaagaactcg gcgcggcgct
      481 ggcgagcggc ctggcgctcg gcgtcttcta gtgcagtgtt cgcgcggccg gcgatttctt
      541 gatctaatag ggtccgcgtg ccaagcaagc agttgctttg tagcgaactg cgtgctcgcc
      601 cggcagcgca cagacccgaa accgcaacga acaagggccc catgccggcc agaccccgaa
      661 cccccgccgg cgccggcgtg aagcgcgccg cacccgaggc gcccgacaac cggcgtgccg
      721 atctggtgcg cgtggccgca cggctgtttc gcgagaaggg cttcgacggc acgacgatcc
      781 gcgacatcgc gcacgcggtg ggcatgcgct cgggcagccc gttctatcac ttcgcgaaca
      841 agcacgagct gctgatggcg gtgatggaag aaggcctgcg cctgggcctg gaacgcaccc
      901 agcaggtgct ggccgacgcc acgctgccgg ccgccgagcg cttcgcacgg ctggtgcgca
      961 cgcagtacgg catcctgcac gacaccggca gcgacttcat cccggtgatg ctgtacgact
     1021 ggcgctcgct gccggcgcag tacaagcgcc gcatcgtcga gctgaaggac cgctacgacg
     1081 cgatctggca gcgcacgctc gacgagctcc atgcgcaggg cctgctgcgc gccgacccgc
     1141 ggctggcgcg gctgatgatc ctgggcgcga tcaacttctc ggccacgtgg taccgcgcga
     1201 agccgcgcgc ggcggcgcgc gtcgacctcg acgaactcgc ggcgcagacg gtcgcgctgg
     1261 tgctgcaccg gaggtgacgt ttggacaaca cgctgctcat cgaacgcgac ggcccggtct
     1321 ggaccgtgac gctgaaccgc cccgcggtcc gcaacgcggt cgacggcccg accgcgcacg
     1381 cactggcgcg ggccttccgt gatttcgatg cggacggtga agcctgcgtt gcggtgctga
     1441 ccggcgccgg cggccagttc tgtgccggcg ccgacctgaa ggccgtggcc agcggccacg
     1501 gcggcaaccc gctcgacgcc gacctgcgca gcgacggccc gatgggtccg acgcggctgc
     1561 agctgggcaa gccggtgatc gccgcggtcg agggccacgc cgtcgccggc ggactcgagc
     1621 tggcgctgtg gtgcgacctg cgcgtggcca gccgcacggc cgtgttcggc gtgttctgcc
     1681 ggcgcttcgg cgtgccgctg atcgacggcg gcacggtgcg cctgccgcgc ctgatcggcg
     1741 aaagtcgcgc gctcgacctg atcctcaccg gccgcgcggt cgcggccgac gaggcgctcg
     1801 cgctggggct ggcgaaccgt gtcgtgccgg ccggcgaagc actggcggtc gcgcgcgaac
     1861 tggcgcgcca gatcgccgcg tttccgcagc aatgcctgcg ccacgaccgc gcgagcgtga
     1921 tggcccagca cggagcgccg ctgcgcgacg cgctcgcgca cgagttcgcg ctcgggctgc
     1981 gcacgctgca gtcgggcgag tcggtcgccg gagcgcagcg cttcagcgac ggcgcggggc
     2041 ggcacgggac gttttcgggc ggatgaccag acaacgggca ggcgcgctcg accgtttcac
     2101 cacgctccag cgcgtacggg gttcgactcc ggctcacacc accttgatga ccacttttcc
     2161 cgtcgcccgc cccgactcgg aatacgcgag cgcgtcacgc acctgctcga gcggaaagac
     2221 cctctccacc agtggtttga tggcaccgct ttccaccagc cgcgtgattt ccctcagctg
     2281 ttctccgtcg gcgcgcatga acaggtactc gtagcgggca ttgcggcgcc gcgctgcggc
     2341 cattgccttt cgggacatga agcggatcgc caggacgatg aactcgttca gcccccagct
     2401 ccgcgcgaat gccgcggacg gcgtaccccc gatgctcacc accacgccgc cgggctttac
     2461 gcactcgaag cacctgacca gcgtgtcgcc gcccgcgctg tccagcacga cgtcgcagtc
     2521 acgggccacg tcctcgaaac gcttgctgac gtaatcgatg gggacgtccg cgccgagacg
     2581 cttcaccaac tccagattgc gctgccccac ggtggtgaaa accgttgccc cgaggtgatg
     2641 ggccaactgg atggcgacgc ttccgacgcc gccgctgccg gcgtgaatca acgcgcgttg
     2701 gccaacgccg agccttccaa gctccaccaa ggcctgccat gccgtcagcg cgaccaatgg
     2761 aagcgaggcg gcctcttcga acgtgacgtt gctgggcttg ggggcggcgg cgccctcgcg
     2821 gaccacggcg aattccgcga acgcgccgat gcggctcttg tccagacgcg cgtagacggc
     2881 atcgcctttc ttgaagcgcg tcacccgctc acccacgtcg accacaacgc ccgacaagtc
     2941 attgcccagc accagcggga aacggtattt cagcaacagc ttcaactttc catcacgcgt
     3001 cttggcatcc agcggattca cgctggcggc gtgtacttga accaagagat ccatggcgcc
     3061 gatctcggga acggcaatct ccgccaccct caccaggtca ttgcttccat agcggtcgat
     3121 gagtgcggct ttcacggaac tccttcgcag tgggccaagc gctctccatg gcgcaaccca
     3181 catcatgggc tttgctgacg tggcccgagg acaatgtaac ccgaccgaac atgcgcgctc
     3241 tgcgttcgcg ctcccacgcc gctcgccgca gcattgccgg tggcagtgcc gcaccgtcac
     3301 gtgtccgaaa acggcgagtc gcgcgtcccc cggcgtcgat aatcgcgcca tggacctcgg
     3361 ccacatcgca ctcgacagcc acgccgcgta ccgcgtcatc accgcgcgcg acgcgcgctt
     3421 cgacggccgg ctgtttgtcg gcgtgacctc gaccggcatc tactgccggc cgatctgccg
     3481 cgtgcgcacg ccgcgcgagc gcaactgccg cttcttcgcg acgccggcgc aggccgaggc
     3541 ggcggcgttc cgcccgtgca tgaagtgccg ccccgagatc gcgcccggcg cgccgcacgc
     3601 gtggaccgtg atggacgcgt cgcgcacgct cgcgcgccag gccgccgatg cgctcgacgc
     3661 gcaggccgcg agcggcgacg cggccagcct cgtccttgtg gccgggcggc tcggcgtcag
     3721 cgaccggcac ctgcgccgca tcttccaggc cgagcacggc gtgacgccgc tgcagtacct
     3781 gcagacgcgc cgcctgctgc t
//
LOCUS       DATMJB010000588         2478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_564895, whole genome shotgun sequence.
ACCESSION   DATMJB010000588 DATMJB010000000
VERSION     DATMJB010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564895"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /gene="era"
                     /locus_tag="VNU48_12500"
     CDS             <1..803
                     /gene="era"
                     /locus_tag="VNU48_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWI12144.1"
                     /translation="QKVSITSAKAQTTRHRITGVRTVDDSQFVFVDTPGFQTKHGAAL
                     NRTLNRTVTGVLPDVDVVLFVVEAGRFGLDDAKVLALMPPGKPVFLIANKLDAVLRRA
                     DLAPWLKGMQERHAFAEFVPLSAKKDADVQRLLGIVRPYLPEQTWFYDADTLTDRSEK
                     FLASEIIREKLFRLTGDELPYTSTVVIDKFEEEGNLRRISASIVVERDAHKGMVIGNG
                     GERLKRIGSEARQELETLLDAKVYIELWVKVRSGWADDEAHLRSYGYE"
     gene            814..1593
                     /gene="recO"
                     /locus_tag="VNU48_12505"
     CDS             814..1593
                     /gene="recO"
                     /locus_tag="VNU48_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWI12145.1"
                     /translation="MATRAPRHAAPLAAYVLHRYDWSESSLILDLFTREQGRLAVAAK
                     GAKRPYSQLRGVLLPFQRLNVTLGKVAAHDAGSSDVQTLRGAEWAGEAAMLTGAALFS
                     GFYLNELLMKLLARHDPHAALFDTYAATLPELTASADETRVQAALRAFELGLLQQTGL
                     LPDLSVVTLTQQPVRGAARYALLPESGVTDAAPGRDAELSGEALIGLQAALEHGSPAA
                     LQHACRSCLAALKLPLRAMIQHQLGATPLRTRQAMADLQSL"
     gene            1640..2443
                     /locus_tag="VNU48_12510"
     CDS             1640..2443
                     /locus_tag="VNU48_12510"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HWI12146.1"
                     /translation="MNPLLTTGLDARRGTTALSVNVNKVALLRNTRSLGVPSVLRAAT
                     FALEAGAHGITVHPRPDERHIRAHDVHELAELMQQWPQAEYNIEGNPFHNLMGFVREL
                     VAKRLPIHQVTFVPDGVEQSTSDHGWQFPQDAQRLAPLVAEARALGVRVSLFMDPAPQ
                     AMAAVRALQADRVELYTESYARAHLAGDAETVLPAFTATARAARAEGLGLNAGHDLNR
                     ANLGDFLRAVPGVQEVSIGHALIADALELGYTETVRDYLRCIHRAGEPA"
BASE COUNT          394 a          902 c          845 g          337 t
ORIGIN      
        1 ggcagaaggt cagcatcaca tcggccaagg cgcagaccac gcggcaccgc atcaccggcg
       61 tgcgcacggt cgacgattcg cagttcgtct tcgtcgacac gccgggcttc cagaccaagc
      121 acggggcggc gctgaaccgt accttgaacc gcaccgtcac cggcgtgctg ccggatgtgg
      181 acgtggtgct gttcgtcgtc gaggccggcc gcttcgggct ggacgatgcc aaggtgctgg
      241 cgctgatgcc gcccggcaag ccggtcttcc tgatcgccaa caagctcgac gcggtgctgc
      301 gccgcgccga cctcgcgccg tggctcaagg gcatgcagga gcgccatgcg ttcgccgaat
      361 tcgtgccgct gtcggcgaag aaggacgccg acgtgcagcg cctgctcggc atcgtcaggc
      421 cctacctgcc ggagcagacc tggttctacg atgccgacac gctgaccgac cgcagcgaga
      481 aattcctcgc cagcgagatc atccgcgaga agctgttccg cctgaccggc gacgaactgc
      541 cctacacctc gaccgtcgtg atcgacaagt tcgaagagga aggcaacctg cgccgcatca
      601 gcgccagcat cgtcgtcgaa cgcgatgccc acaagggcat ggtgatcggc aatggcggcg
      661 agcgcctcaa gcgcatcggc tccgaagcgc gccaggagct cgaaacgctg ctggacgcca
      721 aggtgtacat cgagctgtgg gtcaaggtgc gctcgggctg ggccgacgac gaagcgcacc
      781 tgcgttccta cggctacgag tagcgaagcg cggatggcga cgcgcgcgcc ccgccatgcc
      841 gcgccgctgg cggcctacgt gctgcaccgc tacgactgga gcgaatcgag cctgatcctc
      901 gacctgttca cgcgcgagca gggccgtctg gccgtcgcgg cgaagggcgc gaagcgaccg
      961 tactcgcaac tgcgtggcgt gctgctgccg ttccagcgcc tgaatgtcac gctcggcaag
     1021 gtggcggccc acgacgccgg ctcgagcgac gtgcagacgc tgcgcggtgc cgagtgggcc
     1081 ggcgaagccg cgatgctgac cggcgccgcg ttgttcagcg gcttctacct gaacgaactg
     1141 ctgatgaaac tgctcgcgcg ccacgacccg cacgccgcgc tgttcgacac ctacgccgcg
     1201 acgctgccgg aactcaccgc cagcgccgac gagacgcggg tccaggccgc gttgcgcgcc
     1261 ttcgaactgg gcctgctgca gcagaccggg ctgctgcccg acctgagcgt cgtgaccctg
     1321 acgcagcagc cggtgcgcgg cgccgcgcgc tacgcgctgc tgccggagtc cggcgtcacc
     1381 gacgcggcgc ccggccgcga cgccgagctc agcggcgaag cgctgatcgg cctgcaggcg
     1441 gcgctcgagc acggcagccc ggcggctctc cagcacgcct gcaggtcgtg cctggcggcg
     1501 ctgaagctgc cgctgcgcgc gatgatccag caccagctcg gcgccacgcc gctgcgcacg
     1561 cgccaggcga tggcagacct gcaaagcctg tagtctggcg gtgcacgcgc acgtccggtt
     1621 tgcaccgaga atccgcccca tgaacccgct cctcaccacc ggtctcgatg cgcggcgcgg
     1681 cacgaccgcg ctgtcggtca acgtcaacaa ggtcgcgctg ctgcgcaaca cgcgcagcct
     1741 cggtgtcccg agcgtgctgc gtgcggccac gttcgcgctc gaagccggcg cgcacggcat
     1801 cacggtgcac ccgcggcccg atgaacgcca catccgcgcc cacgacgtcc acgagctggc
     1861 cgagctgatg cagcagtggc cgcaggccga atacaacatc gagggcaatc cgttccacaa
     1921 cctgatggga ttcgtgcgcg agctggtcgc gaagcgcctg ccgattcatc aggtcacgtt
     1981 cgtgcccgac ggcgtcgagc agtccacctc cgaccacggc tggcagttcc cgcaggacgc
     2041 gcagcgcctt gcgccgctgg tcgccgaagc gcgggcgctg ggtgtgcgcg tcagcctgtt
     2101 catggacccc gcgccgcagg cgatggccgc ggtgcgcgcg ttgcaggccg accgcgtcga
     2161 gctctacacc gaaagctacg cccgcgcgca cctggccggc gacgccgaga cggtgctgcc
     2221 ggcgttcacc gcgaccgcgc gcgcggcgcg cgccgagggt ctggggctga acgccggcca
     2281 cgacctgaac cgcgccaacc tgggcgactt cctgcgcgcc gtgcccggcg tgcaggaggt
     2341 ctcgatcggc cacgcgctga tcgccgatgc gctcgaactc ggctacaccg agacggtgcg
     2401 cgactacctg cgctgcatcc accgcgcagg cgagccggca tgaggcgggc cgagcgccgg
     2461 gccgctccca agccggcc
//
LOCUS       DATMJB010000589         5710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_439574, whole genome shotgun sequence.
ACCESSION   DATMJB010000589 DATMJB010000000
VERSION     DATMJB010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439574"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VNU48_12515"
     CDS             complement(<1..815)
                     /locus_tag="VNU48_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI12147.1"
                     /translation="MATTVAAGSKVGQPMTREEKKVIFASSLGTVFEWYDFYLYGTLA
                     AIIGKQFFSNLDPASQTIFALLAFAAGFIVRPFGAIFFGRLGDMIGRKYTFLVTILIM
                     GLSTFIVGILPNFAAIGVAAPVILIGLRLLQGLALGGEYGGAATYVAEHAPQGKRGAF
                     TSWIQTTTTLGLALSLVVILGTRTSVGEEAFADWGWRIPFLVSIVLLAVSVWIRLSMN
                     ESPAFKKMKEEGKTSKAPLTESFGQWRNLKIVILALVGLTMGQAVVWYTGQFYA"
     gene            complement(1065..3224)
                     /locus_tag="VNU48_12520"
     CDS             complement(1065..3224)
                     /locus_tag="VNU48_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HWI12148.1"
                     /translation="MKPAQRLPIAIAALALALTVLALASVALVWATLDAAEREAVAGV
                     LASRAMLVVMWWLAVFGLAAWGLTWWHAQHVEAPARLHEQAQALLAGADGELAAQGSA
                     ANRGLARTLEALARQRTQLQRDIAEQVAEASRGIEQERSRLSALMAELTQSVLVCNRD
                     GRILLYNSRARTQFRDWSRTPATAGGAELIGLGRSVYAVFDRALIAHALETIEQRART
                     GLAQPSAQFVTSAPGGQLLRVQMAPVHDPQRGAAAPGGFVLMLDDITREIEHEAARDR
                     LLLGLTEGSRASLANLQAALEMLELTDLEPPMRERFQHVVRDEVALMTARVQSLAADV
                     LAGMKTRWPMEDMRGAELVAAARRRIEAPGAVRVDSDEVDPALWLHVDSYTLLQALAY
                     LARRLVDEYGLRTLRLRLQGAGPRARLDLVWAGQAMSTETVMSWQMEAMRSAGADATP
                     LTVRDVVERHHGEFWFERERVRHEAFFRFLLPCSEARDAPPPAPLADADGRPEFYDFD
                     LFQTSAQTRELDERLLTELTYTVFDTETTGLAPTDGDEIIQIGATRIVNGRLLRHECI
                     DQLIDPQRPVAAASAAIHGIRAETLAGRPTILEVLPVFHAFALDTVLVAHNAAFDMRF
                     LQLKEAASGLRFDQPVLDTLLLSAWLHPNQASHRLEAIAQRLGVAVLGRHTALGDAIV
                     TAEVFLRLVPLLQAREVRTLGQARAAALQTYQARLTY"
     gene            complement(3221..3601)
                     /locus_tag="VNU48_12525"
     CDS             complement(3221..3601)
                     /locus_tag="VNU48_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI12149.1"
                     /translation="MTTERILIADDEPNILISLEYLMKREGYAVSVARDGDEALAAIR
                     RERPALVLLDVMMPGRSGIEVCQEVRADEALKDTLIVMLTAKGRDTDVAKGLGVGADA
                     YVTKPFSTKELVHKVRQLLEGRAG"
     gene            complement(3598..>5710)
                     /locus_tag="VNU48_12530"
     CDS             complement(3598..>5710)
                     /locus_tag="VNU48_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI12150.1"
                     /translation="TWGLFDGPGDLFARARAVPALHALLNLTPAPGQKFAYEQWFALT
                     LLSMLSVIFLPRQFQMIVVENVAESHLKRAAWVFPLYLLLINLFVLPIALGGLLHFGS
                     GTTDADTFVLSLPLAQGEQALALLVFIGGLSAATGMVIVETIAVSTMVCNDLVMPMLL
                     RTRRFGARAGGDLSRLLLAIRRSAIVTILLLGYVYFAIAGEAYALVSIGLISFAAVAQ
                     FAPAMLAGLYWKGGTRRGALVGLLLGFALWAYTLMLPSVAKSGWLFGELGPAFLRDGP
                     WGIALLRPEQLLGLSGLDNLTHALFWSLLVNGVAFVALSLWRPPAAREASQALLFVDV
                     FRTNVGATAGPVFWRGRAQVRELLPLARRFLGAGRATQLFDDYARQRGVARIEQIPPD
                     AQTVQFVETQLAGAIGSASARVTVAAAVDEEALGLDDVMRILDEASQLRAHSQQLERA
                     TAELRAANEQLQSLDRLKDDFMSSVTHELRTPLTSIRALSEMMADDLDMPRDQRAQFL
                     HIVTAETERLSRLVNQVLDMAKIEAGHAQWHTTDVDLRELLTRAAQTTAELFRERGAS
                     VALELPDALPTLRLDADRLTQVLLNLLSNAAKFLPPRDGRVVVRLRGAEAGAQVEIED
                     NGCGVAPEQQALIFEKFRQGGDAANRPQGTGLGLPISRQIVEHFGGRMWLRSVPGQGA
                     CFGFELPWRAAPEAAMGGHTP"
BASE COUNT          880 a         2014 c         2018 g          798 t
ORIGIN      
        1 gcatagaact ggcccgtgta ccagacgacg gcctggccca tggtgaggcc gaccagcgcc
       61 aggatcacga tcttcaggtt gcgccactgg ccgaacgact cggtgagcgg ggccttggag
      121 gtcttgccct cttccttcat cttcttgaag gccggcgact cgttcatcga caggcggatc
      181 cagacgctga ccgcgagcag cacgatcgag accaggaacg gaatgcgcca gccccagtcg
      241 gcgaacgcct cttcaccgac cgacgtgcgg gtgcccagga tcaccaccag cgagagcgcg
      301 agaccgagcg tggtcgtggt ctggatccag gaggtgaacg caccgcgctt gccttgcggc
      361 gcatgctcgg cgacataggt cgcggcaccg ccgtactcac cgccgagcgc caggccctgc
      421 agcagccgca ggccgatcag gatcaccggt gccgcgacgc cgatcgccgc gaagttgggc
      481 aggatgccga cgatgaaggt cgacaggccc atgatcagga tcgtcaccag gaaggtgtac
      541 ttgcgaccga tcatgtcgcc gagccggccg aagaagatcg cgccgaacgg gcgcacgatg
      601 aagccggcgg cgaacgccag caacgcgaag atggtctgcg acgccgggtc gaggttcgag
      661 aagaactgct tgccgatgat cgccgcgagc gtgccgtaga ggtagaagtc gtaccactcg
      721 aataccgtgc ccagactgga agcgaagatg accttcttct cttcccgggt catgggctgg
      781 ccgactttcg agccggctgc cactgtggtt gccatgttga cgtctcctgt ttggaacttg
      841 aacggcatca ccccctggtg acgcgtgagc ggatgttcgg tgcgccctct gaccgcttgc
      901 ttacacgcag ctgaatcgag gctgacaaac tcgggtccaa ccctgagcga ttttttctcg
      961 atgcgggata tgagcgcttg ccggcgtcga tacgggaaag tcctgagtgg gaagtcgacg
     1021 aacgatcgag gcctgctgct gcgggacgcg cggcgcagac cgggtcaata cgtcaaccgc
     1081 gcctgatagg tctgcagcgc cgcggcgcgg gcctggccga gcgtgcgcac ctccctggcc
     1141 tgcagcagcg ggacgagccg caggaacact tcggccgtca cgatcgcgtc gccgagtgcg
     1201 gtgtgccggc cgagcaccgc gacgccgagg cgctgcgcga tcgcctcgag ccgatgcgaa
     1261 gcctggttcg ggtgcagcca ggccgacagc agcagcgtgt cgagcaccgg ctggtcgaag
     1321 cgcagcccgc tggcggcctc cttgagctgc aggaagcgca tgtcgaaggc cgcgttgtgc
     1381 gcgaccagca cggtgtcgag cgcgaacgcg tggaacaccg gcagcacctc gaggatcgtc
     1441 ggccggcccg ccagcgtctc ggcgcggatg ccgtggatcg ccgccgacgc agcggccacc
     1501 ggccgctgcg ggtcgatcag ctgatcgatg cattcgtggc gcagcagccg gccgttgacg
     1561 atgcgcgtgg cgccgatctg gatgatctcg tcgccgtcgg tcggcgcgag gccggtggtc
     1621 tcggtatcga acaccgtgta ggtcagttcg gtgagcagcc gctcgtcgag ctcgcgcgtc
     1681 tgcgccgagg tctggaacag atcgaagtcg tagaactcgg gccggccgtc ggcgtccgca
     1741 agcggagccg gcggcggcgc gtcgcgggcc tcggagcacg gcagcaggaa gcggaagaag
     1801 gcctcgtggc gcacccgctc gcgctcgaac cagaactcgc cgtgatggcg ctcgaccacg
     1861 tcgcgcacgg tcagcggcgt ggcgtcggcg ccggcactgc gcatcgcctc catctgccag
     1921 ctcatcacgg tctcggtgct catcgcctgg ccggcccaga ccaggtcgag gcgtgcccgc
     1981 gggcccgcgc cctgcaggcg cagccgcagc gtgcgcagcc cgtattcgtc gaccaggcgc
     2041 cgggccaggt acgccagcgc ctgcagcagc gtgtagctgt cgacatgcag ccacagcgcc
     2101 gggtcgacct cgtctgagtc gacgcggacc gcgccgggcg cctcgatccg gcggcgcgcc
     2161 gcggcgacca gttcggcgcc gcgcatgtcc tccatcggcc agcgcgtctt catgccggcc
     2221 agcacgtcgg cggcgagcga ctgcacgcgc gcggtcatca aggcgacctc gtcgcgcacc
     2281 acgtgctgga agcgctcgcg catcggcggc tcgaggtcgg tcagctcgag catctcgagc
     2341 gcggcctgca ggttggccag cgacgcccgg ctgccctcgg tgaggccgag cagcaggcgg
     2401 tcgcgcgccg cctcgtgctc gatctcgcgg gtgatgtcgt cgagcatcag cacgaagccg
     2461 ccgggcgcgg cggcgccgcg ctgcgggtcg tggaccggcg ccatctgcac ccgcagcagc
     2521 tggccgcccg gcgcgctggt gacgaactgt gccgacggtt gcgcgaggcc ggtgcgcgcg
     2581 cgctgctcga tcgtctcgag cgcatgggcg atcagcgcgc ggtcgaagac cgcatagacc
     2641 gagcggccca ggccgatcag ctcggcgccg cccgcggtcg ccggcgtgcg cgaccagtcc
     2701 cggaactggg tccgcgcgcg gctgttgtag agcaggatgc ggccatcgcg gttgcacacc
     2761 agcacgctct gggtcagctc ggccatcagc gccgacagcc ggctgcgttc ctgctcgatg
     2821 ccgcggctgg cctcggcgac ctgctcggcg atgtcgcgct gcagttgcgt gcgctggcgt
     2881 gccagcgctt cgagcgtgcg cgccaggccg cggttcgccg cactgccctg cgccgcgagt
     2941 tcgccgtccg cgccggcgag cagcgcctgg gcctgttcgt gcaagcgcgc cggggcttcg
     3001 acgtgctgcg catgccacca cgtcaggccc caggccgcga ggccgaagac cgcgagccac
     3061 cacatcacga ccagcatcgc gcggctcgcg agcacgccgg cgaccgcctc gcgctcggcc
     3121 gcatcgagtg tggcccagac cagcgcgaca ctggccagcg cgagcacggt gagcgccaac
     3181 gcgagtgccg cgatcgcgat cggcaggcgc tgcgccggct tcatccagcg cggccctcga
     3241 gcagctggcg taccttgtgc accagctcct tggtcgagaa cggcttggtg acgtaggcgt
     3301 cggcgccgac gcccaggccc ttggcgacat cggtgtcgcg gcccttcgcg gtcagcatca
     3361 cgatcagcgt gtccttcagc gcctcgtcgg cgcggacctc ctggcagacc tcgatgccgg
     3421 agcgcccggg catcatcacg tcgagcagca ccagcgccgg ccgctcgcgc cggatcgccg
     3481 cgagggcctc gtcgccgtcg cgcgcgacgc tgacggcgta gccctcgcgc ttcatcaggt
     3541 actcgagcga gatcaggatg ttcggttcgt cgtcggcgat caggatgcgc tcggtggtca
     3601 tggcgtgtgt cctcccatcg cggcctcggg cgctgcgcgc cacggcagct cgaacccgaa
     3661 gcatgcgccc tggcccggca ccgagcgcag ccacatccgc ccgccgaagt gctcgacgat
     3721 ctggcggctg atcggcaggc ccaggccggt gccctgcggg cggttcgcgg cgtcgccgcc
     3781 ctggcggaac ttctcgaaga tcagcgcctg ctgctcgggc gcgacgccgc agccgttgtc
     3841 ctcgatctcg acctgcgcgc cggcctcggc gccgcgcagc cgcacgacca cccggccgtc
     3901 gcgcggcggc aggaacttgg ccgcgttgga cagcaggttc agcagcacct gggtcagccg
     3961 gtcggcatcc agccgcaagg tcggcagcgc gtcgggcagt tcgagcgcga cgctcgcgcc
     4021 gcgctcgcga aacagctcgg cggtggtctg cgcggcgcgc gtcagcagct cgcgcaggtc
     4081 gacgtcggtg gtgtgccact gcgcgtggcc ggcttcgatc ttggccatgt ccagcacctg
     4141 gttgaccagg cggctcaggc gctcggtctc ggcagtgacg atgtgcagga actgcgcgcg
     4201 ctggtctcga ggcatgtcga ggtcgtcggc catcatctcg gacagcgctc ggatcgaggt
     4261 cagcggcgtg cgcagctcgt gcgtcaccga cgacatgaag tcgtccttca gccggtccag
     4321 gctttgcagc tgctcattgg cggcgcgcag ctcggcagtg gcgcgttcga gttgctgcga
     4381 atgcgcacgc agctgcgagg cctcgtcgag gatgcgcatc acgtcgtcca ggccgagcgc
     4441 ctcctcgtcg accgccgcgg ccaccgtcac ccgcgccgac gcgctgccga tcgcaccggc
     4501 cagctgggtc tcgacgaact gcaccgtctg cgcatcgggc gggatctgct cgatgcgcgc
     4561 gacgccgcgc tggcgtgcgt agtcgtcgaa cagctgggtc gcgcggccgg cgccgaggaa
     4621 gcggcgggcc agcggcagca gttcgcgcac ctgcgcccgg ccgcgccaga acacgggccc
     4681 ggccgtcgcg ccgacgttgg tgcggaacac gtcgacgaac agcagcgcct ggctggcctc
     4741 gcgcgccgcg ggcgggcgcc acagcgacag cgcgacgaac gcgaccccgt tgacgagcag
     4801 gctccagaac agcgcgtggg tcaggttgtc gagcccgctc agaccgagca gttgctccgg
     4861 cctgagcaag gcgatgcccc atggcccgtc gcgcaggaac gcaggcccga gctcgccgaa
     4921 gagccagccc gacttcgcca ccgacggcag catcagcgtg taggcccaca gcgcgaagcc
     4981 gagcagcagg ccgacgagcg cgccgcgccg cgtgccaccc ttccagtaca gcccggccag
     5041 catcgccggc gcgaactggg ccaccgcggc gaagctgatc aggccgatgc tgaccagcgc
     5101 ataggcctcg ccggcgatcg cgaagtagac gtagccgagc agcaggatcg tgacgatcgc
     5161 actgcgccgg atcgccagca gcagccggct caggtcgccg ccggcgcggg cgccgaagcg
     5221 gcgcgtgcgc agcagcatcg gcatcaccag gtcgttgcag accatcgtcg agaccgcgat
     5281 cgtctcgacg atcaccatgc cggtggccgc cgacagcccg ccgatgaaca ccagcagcgc
     5341 cagcgcctgc tcgccctgcg caagcggcag cgacagcacg aaggtgtcgg cgtccgtggt
     5401 gccggacccg aagtgcagca gcccgccgag cgcgatcggc agcacgaaca ggttgatcag
     5461 cagcaggtac agcgggaaca cccaggcagc gcgcttcagg tgcgactcgg cgacgttctc
     5521 gaccacgatc atctggaact ggcgcggcag gaagatcacc gacagcatcg acagcagcgt
     5581 cagcgcgaac cattgctcgt aggcgaactt ctgccccggc gcgggcgtca ggttcagcag
     5641 cgcgtgcagc gccggcaccg cgcgggcgcg cgcgaacagg tcgcccgggc cgtcgaacag
     5701 gccccaggtc
//
LOCUS       DATMJB010000590         3950 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_262011, whole genome shotgun sequence.
ACCESSION   DATMJB010000590 DATMJB010000000
VERSION     DATMJB010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262011"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /locus_tag="VNU48_12535"
     CDS             <1..1564
                     /locus_tag="VNU48_12535"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HWI12151.1"
                     /translation="GYFNAEVGAERAGTEAGLPLLRVTVEPGARTTVHAFSLEASGAL
                     QTAAAAGDAAARRELDALRRLWPLRPGDPFRQSAWADAKNATLARLRADGYAAATWSS
                     TNARVDAPENRVDLAVVLDSGPLYRLGAVRIEGLQRYDESAVRQLSTFNAGDPYSEKT
                     LLDFQERLQKLGLFEGASVEIDADPATAAAAPVLVHVKEQSLQQATIGIGYSANTGPR
                     LSLEHTQRRLFGSRWIAKNKFELGPDNKHWEGELTSYPLAGLYRNLVAGSATKLLAAD
                     QQLLSWNARVGRTRDTERLERLYFAELTHARLDSAALTSQAEAASGNYQWVFRRVDNV
                     LLPTQGVTASAQVALGYARGNRAVLGAPLETAKGPFSRLYARLTWYEPFGASWYGTAR
                     VEAGEVFAHSAVGIPDTLLFRAGGEDSVRGYGYRTLGPEIAGVTISGRTLLTGSIEVA
                     RPISPKYPAYWWAAFVDAGNAADRWSELKPALGYGVGLRWRSPVGPLRVDLAYGQQVH
                     QVRMHFSVGIVF"
     gene            1564..>3950
                     /locus_tag="VNU48_12540"
     CDS             1564..>3950
                     /locus_tag="VNU48_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12152.1"
                     /translation="MDERIAPADAPPARRSGVWRAVLALLLVVLAVAIGLASAGWWAL
                     RSERGSAWLLSLLPGVQVEAPQGRLLGDFAARRATIRLPGGDDRVVIDGLAWRGLRVE
                     RAPAPLWARVTLASLEARRVELAFAPRSNAKPMAAPADLRLPIELDLRALHIGELHAQ
                     ALGTQPVRELGAAVHLGANGGAEHRVDGLVLRWDRLQASGHARIASSAPLALDASLML
                     TQQAADALPAWNASATLAGPLSAPLLQATLRASPAPQHPAQALDARATLHPFTAWPLG
                     DLTASTEALDLSAFHSAAPATALTGAAVATSTASDQAAQVSLHLSNALAGRWNEARLP
                     VALLALVLAARPGRPRELALHSFDAELGTLQASAGRLRGSGAWSPERWNLAADLSALQ
                     PARLDARAPAMQLDGAVTLAGGASIAAPIDLKTTLAGVLVERSRTRRVQLELDAALQP
                     QRVELRTLQASAGGARASLSGLLSQTSTEAPWSVKSQLALVDFDPAVWWPGADDAPWR
                     RATHRLNAKGSVDLAVPVNATAQAWPQALTALRGQATLTLADSVLAGVPLHGEISLHS
                     AGSGPARALVSLDADGNALRAQGQVATSGNGAGDVWDLSLDGAALNRLAPVFKMLQPT
                     GADTTLAGTLHAKAHVSGRWPALATQGEFDANALQVGALAVQSAQARWTLGSSAAAPM
                     QARVSLTQLSLAQGTAPGPSIETLQLQLDGSGRAHTLELRAASKARPPAWTDSLQPGA
                     APAPAGARTVALLQARGGLVETAGTAPAGWRGTLQQLDLRSDASGAAPLLHTQDVA"
BASE COUNT          520 a         1473 c         1475 g          482 t
ORIGIN      
        1 gggctacttc aacgccgagg tcggcgccga gcgcgccggc accgaggccg gcctgccctt
       61 gctgcgcgtg acggtcgagc cgggtgcgcg cacgaccgtg cacgcgttca gcctcgaggc
      121 cagcggcgca ttgcagaccg ccgccgcggc cggcgacgcc gcagcgaggc gcgagctcga
      181 cgccctgcgg cgcctgtggc cgctgcgccc cggcgacccg ttccggcaga gcgcctgggc
      241 cgacgcgaag aacgcgacgc tggcgcggct gcgcgccgac ggctacgcgg ccgccacctg
      301 gagcagcacg aacgcgcgcg tcgacgcgcc cgagaaccgc gtcgacctcg cggtcgtgct
      361 cgacagcggg ccgctgtacc ggctcggcgc ggtgcgcatc gagggcctgc agcgctacga
      421 cgagagcgcg gtgcgccagc tctcgacctt caacgccggc gacccgtaca gcgagaagac
      481 cctgctcgat tttcaggagc ggctgcagaa gctcggcctg ttcgaaggcg cgtcggtcga
      541 gatcgatgcc gacccggcca cggccgcggc ggcgccggtg ctggtgcacg tcaaggagca
      601 gtcgctgcag caggcgacga tcggcatcgg ctacagcgcc aacaccgggc cgcggctgtc
      661 gctcgagcac acgcagcggc gcctgttcgg cagccgctgg atcgcgaaga acaagttcga
      721 gctcggcccc gacaacaagc actgggaggg cgagttgacc tcttacccgc tggccgggct
      781 gtaccgcaac ctggtcgcag gcagcgcgac caagctgctc gccgccgacc agcagctgct
      841 gagctggaac gcgcgcgtcg gccgcacccg ggacaccgag cgcctcgaac gcctgtactt
      901 cgccgaactg acgcacgcgc gcctggacag cgccgcgttg accagccagg ccgaggcggc
      961 ctccggcaac taccagtggg tcttccggcg ggtcgacaac gtgctgctgc cgacccaggg
     1021 agtgacggcc tcggcgcagg tcgcgctcgg ctacgcacgc ggcaaccgtg cggtgctcgg
     1081 cgcgccgctc gaaaccgcga agggcccgtt ctcgcgcctg tatgcgcggc tgacctggta
     1141 cgagccgttc ggcgccagct ggtacggcac ggcgcgcgtc gaggccggcg aggtgttcgc
     1201 gcacagcgcg gtcggcatcc ccgacacgct gctgttccgc gccggcggcg aggattcggt
     1261 gcgcggctac ggctaccgca cgctcggccc ggagatcgcc ggcgtcacga tcagcgggcg
     1321 tacgctgctg accggcagca tcgaggtcgc gcggccgatc tcgccgaaat accccgcgta
     1381 ctggtgggcc gcgttcgtcg acgccggcaa cgccgccgat cgctggagcg agctgaagcc
     1441 ggcgctcggc tacggtgtcg gcctgcggtg gcgcagcccc gtcggcccgt tgcgcgtcga
     1501 cctcgcctac ggccagcagg tgcaccaggt gcgcatgcat ttcagcgtcg ggatcgtgtt
     1561 ctgatggacg agcgcatcgc tccggccgat gcgccaccag cgcggcgcag cggcgtctgg
     1621 cgcgcggtgc tcgcgctgct gctcgtcgtg ctggcggtcg cgatcggcct cgcgtccgcc
     1681 ggctggtggg cgctgcgcag cgagcgcggc agcgcgtggc tgttgtcgct gctgcccggc
     1741 gttcaggtgg aagcgccgca gggccggctg ctcggcgact tcgcggcgcg gcgcgcgacg
     1801 atcaggctgc cgggcggcga cgatcgggtc gtgatcgacg gcctggcgtg gcgcggcctg
     1861 cgcgtcgagc gcgcgccggc gccgctgtgg gcgcgggtca cgctggcctc gctcgaagcg
     1921 cggcgcgtcg agctcgcgtt cgcgccgcgc tcgaacgcca agccgatggc cgcaccggcc
     1981 gacctgcggc tgccgatcga actcgatctg cgtgcgctgc acatcggcga actgcacgcg
     2041 caggcgctcg gcacgcagcc ggtccgcgag ctcggcgccg ccgtgcacct gggtgcgaac
     2101 ggcggtgccg aacaccgcgt cgacggcctc gtgctgcgct gggaccggct gcaggccagc
     2161 ggccacgcgc gcatcgcgag cagcgcaccg ctggcgctcg atgcgtcgct gatgctcacg
     2221 caacaggcgg ccgatgcgct gccggcctgg aacgcgagcg cgaccttggc cggcccgctc
     2281 agcgcgccgt tgctgcaggc gaccttgcgc gcatcgcccg cgccgcagca cccggcccag
     2341 gcactcgacg cgcgcgccac gctgcacccg ttcaccgcct ggccgctcgg cgatctgacg
     2401 gccagcaccg aggcgctgga cctttcggcc ttccacagtg ccgcgccggc caccgcattg
     2461 accggcgcgg cggtcgccac cagcaccgct tccgaccaag cagcgcaggt atcgctgcat
     2521 ctgagcaatg cgctggccgg gcgctggaac gaagctcggc tgccggtcgc gctactggca
     2581 ctggtgctgg ccgcgcgccc cggccggccg cgcgagctgg cgctgcacag cttcgacgcc
     2641 gagctcggca cgctgcaggc cagcgcgggc cggctgcgcg gcagcggtgc ctggtcgccc
     2701 gaacgctgga acctcgcggc ggacttgagc gcgctgcagc cggcgcggct ggacgcgcgc
     2761 gcaccggcga tgcaactcga cggggcggtg acgctcgcgg gcggtgcaag catcgccgcg
     2821 cccatcgatc tgaagaccac gctcgccggc gtgctggtcg agcgcagccg cacgcgtcgg
     2881 gtgcagctcg agctcgacgc cgcgctgcag ccgcaacgcg tcgagctgcg cacgctgcag
     2941 gcgagcgcgg gtggtgcgcg cgcgagcctg tcgggcctgc tgtcgcagac ctcgaccgag
     3001 gcgccatgga gcgtgaagtc gcagctggcg ctggtcgatt tcgaccccgc ggtctggtgg
     3061 ccgggcgccg acgacgcgcc gtggcgccgt gccacacatc gcctgaacgc gaaaggcagc
     3121 gtcgacctcg cggtcccggt gaacgcgacg gcgcaggcgt ggccacaagc gctgaccgca
     3181 ttgcgcggcc aggcaacgct gacgcttgcc gacagcgtgc tcgccggcgt gccgctgcac
     3241 ggcgagatca gcctgcacag cgccggcagc gggccggccc gcgcgctggt cagcctcgat
     3301 gccgacggca atgcactgcg cgcgcaaggg caggtcgcga cctcgggcaa tggcgccggc
     3361 gacgtctggg acctgagcct ggacggtgcc gcgctgaacc gcctcgcgcc ggtcttcaag
     3421 atgctccaac ccaccggcgc cgacacgacg ctggccggta cgctgcacgc gaaggcgcac
     3481 gtcagcgggc gctggccggc gctggcgacg cagggcgagt tcgacgcgaa cgcgctgcag
     3541 gtcggcgcgc tcgccgtgca gtcggcgcaa gcgcgctgga cgctcggcag cagcgccgcc
     3601 gcaccgatgc aggcgcgggt gagcctgacg cagctgagcc tcgctcaggg caccgcgccg
     3661 gggccgtcga tcgagacctt gcaattgcag ctcgacggca gcggccgcgc ccacacgctg
     3721 gagctgcgcg ccgcgtcgaa ggcgcggccg ccggcctgga ccgactcgct gcagcccggc
     3781 gcggcaccgg cgcccgccgg ggcgcgcacc gtcgcgctgc tgcaggcgcg cggcgggctg
     3841 gtcgagaccg ccggcaccgc gccggccggc tggcgcggca cgctgcagca gctcgacctg
     3901 cgcagcgacg cgagcggcgc cgcgccgctg ctgcacacgc aggacgtcgc
//
LOCUS       DATMJB010000591         3891 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_742270, whole genome shotgun sequence.
ACCESSION   DATMJB010000591 DATMJB010000000
VERSION     DATMJB010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_742270"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VNU48_12545"
     CDS             <1..916
                     /locus_tag="VNU48_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWI12153.1"
                     /translation="GCNHRCSFCIIPSMRGDLVSRPIGDVLGEAQRLFEAGVKELLVI
                     SQDTSAYGVDVKFRTGFWDGRPVKTHMTDLCEKLAELAAAHGAWVRLHYVYPYPHVDQ
                     ILPLMAGGAILPYLDVPFQHSHPDVLRRMKRPASGEKNLERLQRWREACPEIVIRSTF
                     IAGFPGETEAEFEHLLDFMREARIDRAGCFAYSAVDGATANALPGMLPVELREERRAR
                     FMAVAEGVSAAKLQQRVGATMQVLVDSAPALGRKGGVGRSYADAPEIDGSVTLLPPEK
                     ASKTLRVGEFTRVKIVGTQGHDLVGLPI"
     gene            965..2344
                     /locus_tag="VNU48_12550"
     CDS             965..2344
                     /locus_tag="VNU48_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019719956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HWI12154.1"
                     /translation="MQHEFDAIVIGAGQSGPPLAARFAKAGMKVALIEREHLGGTCAN
                     DGCIPTKTLVASARAAHVARHAAEFGVRIDGAVRVDMRAVKARKDAVVDESIASLTTW
                     LQGLPTLTLFRAQARFIAPNRVRVGDDELSAPKIFINTGGRPTVPDWPGIHDVPYLTN
                     TSMMALDRLPAHLVVVGGSYIGLEFAQMYRRFGSAVTVLEAAPRLIAREDAEVCDALR
                     ALLEAEGVAVHVGVDHLAMARDPAGVRVSYELAGAAQAVTGSDVLLAVGRRPNTDGLG
                     LEAAGVKLDARGYIAVDDELRTSATGVWALGDVNGKGAFTHTSYNDFEIVAANLLDGG
                     SRRVSDRVSAYALYTDPPLGRIGMSETEVRASGRAALVATLPMARVGRARERGETSGF
                     MKVLVDAQTQRLLGAALFGIEADEVVQLLLLAMAADLPYPRIAQTMWIHPTVTELLPT
                     LFAALKPLT"
     gene            2354..3562
                     /locus_tag="VNU48_12555"
     CDS             2354..3562
                     /locus_tag="VNU48_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HWI12155.1"
                     /translation="MSEHDLPERAIETALIHHPYRPPSGFEAPQPGVFKASTVIFAST
                     AAMRARNWKEKVGYTYGLHGTPTTFTLEERIATLEGGLHTVLVPSGLAAIALTDMALL
                     RAGDEVLIPDNAYNPSKELARHELAGWGITHRFYDPMDADALAAMLTPATKLVWIEPP
                     GSVTMEFPDLTRLVRVARSQGALVALDNTWGAGLAFNPFRLEGEGADALGADISVHAL
                     TKYPSGGGDVLMGSVTTRDPALHLKLNAAHMRMGWGVAANDVETVLRALPSLALRYAA
                     ADAAGRALARWWSARPEVTQVLHPALEGSPGHAHWQHLCTRSAGLFSIVFDERYTARQ
                     VDAFVDALKLFRIGFSWAGPVSLVVPYDLGAMRKRARWRGTLVRFSIGLEAVDDLIAD
                     AAQALAALAG"
     gene            complement(3569..>3891)
                     /locus_tag="VNU48_12560"
     CDS             complement(3569..>3891)
                     /locus_tag="VNU48_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWI12156.1"
                     /translation="MRGDSVSERHFAEPQAFRPERWLAEAGPAQAASTSAAKRVSMPF
                     GAGPRVCPGRYLALLEIKMAMAMLLAHFDIVAVDTPDGAEAAEHMAFTMMPVGLRMRL
                     RARA"
BASE COUNT          562 a         1392 c         1389 g          548 t
ORIGIN      
        1 aggctgcaac caccgctgct cgttctgcat catcccgagc atgcgcggcg acctggtctc
       61 gcgcccgatc ggcgatgtgc tcggcgaagc acaacgtctg ttcgaggccg gcgtgaagga
      121 actgctggtg atcagccagg acacgagcgc ctacggcgtc gacgtcaagt tccgcaccgg
      181 cttctgggac ggccgcccgg tcaagaccca catgaccgac ctgtgcgaga agctcgccga
      241 gctcgccgcc gcccacggcg cgtgggtgcg gctgcactac gtctacccct atccgcacgt
      301 cgaccagatc ctcccgctga tggccggcgg cgcgatcctg ccgtacctgg acgtgccgtt
      361 ccagcattcg caccccgacg tgctcaggcg catgaagcgc ccggccagcg gcgagaagaa
      421 cctcgagcgg ctgcagcgct ggcgcgaggc ctgccccgag atcgtgatcc gcagcacctt
      481 catcgccggc tttccgggcg agaccgaggc ggagttcgag cacctgctcg acttcatgcg
      541 cgaggcccgc atcgaccgtg ccggctgctt cgcgtactcg gccgtcgacg gcgcgaccgc
      601 gaacgcgctg cccggcatgc tgcccgtcga actgcgcgag gaacgccgcg cgcgcttcat
      661 ggcggtggcc gaaggcgtgt cggccgccaa gctgcagcag cgcgtcggcg cgacgatgca
      721 ggtgctggtc gactcggcgc cggcgctggg ccgcaagggc ggggtggggc gttcgtacgc
      781 cgacgcgccc gagatcgacg gcagcgtcac gctgctgccg ccggagaagg cgtcgaagac
      841 cttgcgcgtc ggcgagttca cgcgcgtgaa gatcgtcggt acgcaagggc acgacctcgt
      901 cgggctgccg atctgacgcg gcgcacgctg gcgtagagtg ggcggctcac ttcgaggagc
      961 cgcgatgcag cacgagttcg acgcgatcgt catcggcgcc ggccagtccg ggccgcccct
     1021 ggccgcgcgc ttcgcgaagg ccggcatgaa ggtcgcgctg atcgagcgcg agcacctcgg
     1081 gggcacctgt gccaacgacg gctgcatccc gaccaagacc ctggtcgcga gcgcgcgcgc
     1141 cgcgcacgtc gcgcgccatg cggccgagtt cggcgtgcgc atcgacggcg cggtgcgcgt
     1201 cgacatgcgc gcggtcaagg cgcgcaagga tgcggtcgtc gacgaatcga tcgcgagcct
     1261 gacgacctgg ctgcagggcc tgccgacctt gaccctcttc cgcgcgcagg cgcgattcat
     1321 cgcaccgaac cgggtgcgcg tcggcgacga tgaactgagc gcgccgaaga tcttcatcaa
     1381 caccggcggc cggccgaccg tgcccgactg gcccggcatc cacgacgtgc cgtacctgac
     1441 caacacctcg atgatggcgc tcgaccgact gccggcacac ctggtcgtcg tcggcggcag
     1501 ctacatcggc ctggaattcg cgcagatgta ccggcgcttc ggcagcgcgg tcacggtgct
     1561 cgaggcggcg ccgcgtctga tcgcgcgcga ggatgccgag gtctgcgacg cgctgcgcgc
     1621 gctgctcgag gccgagggcg tcgcggtcca tgtcggtgtc gatcatctgg cgatggcacg
     1681 cgacccggcc ggcgtgcgcg tcagctacga gctggcgggc gcggcgcagg cagtgacggg
     1741 cagcgacgtg ctgctcgcgg tcgggcgccg gccgaacacc gacggcctcg gcttagaggc
     1801 cgccggcgtc aagctcgatg cgcgcggcta catcgccgtc gacgacgaac tgcgcaccag
     1861 cgcgacgggc gtctgggcgc tcggcgacgt gaacggtaag ggcgcgttca cgcacacctc
     1921 gtacaacgac ttcgagatcg tggccgcgaa cctgctcgac ggcgggtcgc ggcgcgtctc
     1981 ggaccgcgtc agtgcctacg cgctgtacac cgacccgccg ctcggccgca tcggcatgag
     2041 cgagaccgag gtgcgcgcga gcggccgcgc ggcgctggtc gccacgctgc cgatggcgcg
     2101 cgtcggccgc gcgcgcgagc gcggcgagac gagcggcttc atgaaggtgc tggtcgacgc
     2161 gcagacgcag cgcctgctcg gcgcggcgct gttcggcatc gaggccgacg aggtcgtgca
     2221 actgctgctg cttgcgatgg ccgccgacct gccgtacccg cgcatcgcgc agacgatgtg
     2281 gatccacccg accgtgaccg agctgttgcc cacgctcttc gcggcgctga aaccgctgac
     2341 ctaaactgcg ccgatgagcg aacacgacct gcccgagcgc gcgatcgaga ccgcgctgat
     2401 ccatcatccg taccggccgc cgagcggctt cgaggcgccg cagcccggcg tcttcaaggc
     2461 ctcgacggtg atcttcgcca gcaccgccgc gatgcgcgcg cgcaactgga aagagaaggt
     2521 cggctacacc tacggcctgc acggcacgcc gaccacgttc acgctcgagg agcgcatcgc
     2581 cacgctggaa ggcgggctgc acacggtact ggtgccgagc ggcctggccg cgatcgcgct
     2641 gaccgacatg gccctgctgc gcgcgggcga cgaggtgctg atccccgaca acgcctacaa
     2701 ccctagcaag gaactggcgc gccacgagct cgcgggctgg ggcatcacgc accgcttcta
     2761 cgacccgatg gacgccgacg cgctcgcggc gatgctcacg ccggcgacca aactggtctg
     2821 gatcgagccg cccggctcgg tgacgatgga gttccccgac ctgacgcggc tggtgcgcgt
     2881 ggcgcgctcg cagggcgcgc tggtcgcgct cgacaacacc tggggtgccg gtctcgcgtt
     2941 caacccgttc cgcctcgaag gcgagggcgc cgacgcgctc ggtgccgaca tctcggtgca
     3001 cgcgctgacc aagtatccgt ccggcggcgg cgacgtgctg atgggctcgg tgacgacgcg
     3061 cgatccggcg ctgcacctga agctcaacgc cgcccacatg cgcatgggct ggggcgtggc
     3121 cgccaacgac gtcgagacgg tgctgcgcgc gctgcccagt ctggcgctgc gctatgccgc
     3181 ggccgacgcg gccggccgcg cgctcgcgcg ctggtggtcg gcgcggcccg aggtcacgca
     3241 ggtgctgcac ccggcgctcg aaggctcgcc gggtcatgcg cactggcagc atctgtgcac
     3301 ccggtcggcc ggcctgttct cgatcgtgtt cgacgagcgc tacacggcac gccaggtcga
     3361 tgccttcgtc gatgcgctga agctgttccg gatcggcttc tcgtgggccg gcccggtcag
     3421 cctggtcgtg ccctacgacc tcggcgcgat gcgcaagcga gcgcgctggc gcggcacgct
     3481 ggtgcgcttc tcgatcggcc tggaggcggt cgacgacctg atcgccgacg cggctcaggc
     3541 gctcgcggcg ctcgccggct gagcggcgtc aggcgcgcgc ccgcagccgc atccgcaagc
     3601 ccaccggcat catcgtgaat gccatgtgct cggcggcctc ggcgccgtcg ggcgtatcga
     3661 cggccacgat gtcgaagtgc gccagcagca tcgccatggc catcttgatc tcgagcagcg
     3721 cgaggtagcg gcccgggcac acgcgcggcc cggcgccgaa gggcatcgac acgcgcttgg
     3781 cggcgctggt gctcgcggcc tgcgctggcc cggcttcggc gagccagcgt tcaggccgga
     3841 acgcctgcgg ctcggcgaag tgacgctcgc tgacgctgtc gccgcgcatc a
//
LOCUS       DATMJB010000592         4053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_296674, whole genome shotgun sequence.
ACCESSION   DATMJB010000592 DATMJB010000000
VERSION     DATMJB010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296674"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..317)
                     /locus_tag="VNU48_12565"
     CDS             complement(111..317)
                     /locus_tag="VNU48_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12157.1"
                     /translation="MTGHDHTNPPQGYVQLTEGRIRTTDLVFGKTSSRWSEPHAFVVG
                     SAVGEDGTRVARRLPPEPKSRSRN"
     gene            complement(411..941)
                     /locus_tag="VNU48_12570"
     CDS             complement(411..941)
                     /locus_tag="VNU48_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HWI12158.1"
                     /translation="MIAGAARRGPTGSRSNAAATGFTLIELMVAVAVVAILATLALPS
                     ARGQLVRGQIVEAMKIAEIAKAPIAAAWGATHALPADNAAAGLPPAEKIVGNLVTSVL
                     VEGGAIHVTFGNKANPAIAGKTLSLRPAVVEDAAIVPIAWLCGQAAAVDKMVAKGVDR
                     TTVEANLLPLNCRAGG"
     gene            complement(938..1780)
                     /locus_tag="VNU48_12575"
     CDS             complement(938..1780)
                     /locus_tag="VNU48_12575"
                     /inference="COORDINATES: protein motif:HMM:NF020395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family c-type lysozyme inhibitor"
                     /protein_id="HWI12159.1"
                     /translation="MKSHHQPASGRSAALAAALLVAASAAAQTGLDAPTLRQFGGRYA
                     VDCASAAAPRLRVDADALLVEQGNQRMTGRNAMSSYSYFGNSPPPKDFQVALMGEVRG
                     GLQLLFMVYADKGGPYIRLEGDAKVAAALGKALMTSRFRRCEAPSAPVAAPPPTPAAA
                     PRAAAPGAAPELGTLVEDAAFKRAYLKAIGPLAAQRWLARFEGPAPSTRMQTFDGTPY
                     VVIGICKAHDCYDHNASFLYSAAQQRVIGLVQQNGVKTLVGAPGAALAPQLERLWQSQ
                     WRQK"
     gene            1825..2202
                     /locus_tag="VNU48_12580"
     CDS             1825..2202
                     /locus_tag="VNU48_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12160.1"
                     /translation="MHFVAKHRRARQGSIIGSFPRLNAAAAMFERVLAGIVLAVCALL
                     LLRLTMSVPRRYRFDAAWRRIWLALRRAGLRAWHWRAARRDARRAREVADEAIRRARD
                     RGDWDGNVYTPKSFRKPPRDKMH"
     gene            2259..2558
                     /locus_tag="VNU48_12585"
     CDS             2259..2558
                     /locus_tag="VNU48_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12161.1"
                     /translation="MVRLDIPADALRERTFEIFVRLAVANKAHRTDATHALRVLVDGV
                     LEWSRSAATHVDGSDSLDLRLRRSVPPGRPLRLSASCELHGAQRVRLSIDAEEEG"
     gene            complement(2565..3845)
                     /locus_tag="VNU48_12590"
     CDS             complement(2565..3845)
                     /locus_tag="VNU48_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HWI12162.1"
                     /translation="MNAAIIFVLLIVLMLTGMPISISLGLTVLTFLFTMTDVPIASVA
                     LKLFTGIEKFEIMAIPFFILAGNFLTHGGVARRMIRFATSMIGHYYGGLGLAGVMACA
                     LFAAVSGSSPACVVAIGSVILPAMVKQGFPRNFGAGIVTTSGALGILIPPSIAMVMYS
                     VSTNVSVGAMFIAGILPGIAMATALGLTTWFIARKNDYPRMPRASWGERWTAFRESVW
                     GLLLIVVVMGGIYTGIFTPTEAAAMAAVYAFIVAVFIYKDMPLKRIPKVLLDSASMSA
                     MLLYIITNAVLFSFLMTSENIPQAMADWMIAQGFGPVAFLLVVNILLLLAGNVMEPSS
                     IILIMAPILFPVAMKLGIDPIHFGILIVVNMEVGMCHPPVGLNLYVASGITKMGISEL
                     TVAVWPWLATMLVFLVIVTYWPPLSTWLPHLLMK"
     gene            complement(3842..>4053)
                     /locus_tag="VNU48_12595"
     CDS             complement(3842..>4053)
                     /locus_tag="VNU48_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008325756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HWI12163.1"
                     /translation="ADLEMPMWIVYLCVPLGTSLMCYRFLQVAWSFSRTGELPHHDHG
                     HVEGIEDVAAVAVPSDQELAHGDRK"
BASE COUNT          630 a         1431 c         1396 g          596 t
ORIGIN      
        1 catccggcgg gtcggcatga acccatggtc cgcaacacca gatccccggt caaccccgcg
       61 ttcaggggcg tgcgccgccg cggcggcgcg gcggcgcgca cgcgtgcgcg tcagttgcgg
      121 ctgcggctct tcggctcggg cggcaggcgg cgcgcgaccc gggtgccgtc ctcgccgacg
      181 gcactgccga ccacgaacgc atgcggttcg ctccagcggc tggaggtctt gccgaagacg
      241 aggtcggtgg tgcggatgcg gccttcggtg agctgcacat agccttgcgg cgggttcgtg
      301 tggtcgtgtc cggtcatggg agatttcgag tggtgtaggc agcgattgtc caacacatcc
      361 ggaatggccc cacgctcggc cgcggccgcg acatcgtgcg cacccgcgtg tcacccgccg
      421 gcacggcaat tgagtggcag cagattggcc tcgaccgtcg tgcggtcgac ccccttcgcg
      481 accatcttgt cgaccgccgc cgcctgcccg cagagccagg cgatcggcac gatcgcggcg
      541 tcctcgacga ccgccgggcg caggctcaag gtcttgcccg cgatcgccgg gttcgccttg
      601 ttgccgaagg tcacgtggat cgcaccgccc tcgaccagca ccgaggtgac gaggttgccg
      661 acgatcttct cggccggcgg cagaccggcc gcggcgttgt cggccggcag cgcgtgcgtc
      721 gcgccccagg ccgcggcgat cggcgccttc gcgatctcgg cgatcttcat cgcctcgacg
      781 atctggccgc gcaccagctg cccgcgcgcg ctcggcaacg cgagcgtcgc gaggatcgcg
      841 acgacggcca ccgcgaccat cagctcgatc agcgtgaagc ccgtcgccgc ggcgttcgat
      901 cggctgcccg tcgggccacg gcgcgcggct cccgcgatca cttctggcgc cactgcgatt
      961 gccacaggcg ctcgagctgc ggcgccaacg ccgcgcccgg cgctccgacc agcgtcttga
     1021 ccccgttctg ctggaccagc ccgatcacgc gctgctgcgc ggccgagtac aggaagctcg
     1081 cgttgtggtc gtagcagtcg tgcgccttgc agatgccgat cacgacgtac ggcgtgccgt
     1141 cgaaggtctg catccgcgtc gacggcgccg ggccttcgaa gcgcgcgagc cagcgctgcg
     1201 ccgccagggg cccgatcgcc ttcaggtagg cacgcttgaa tgccgcgtct tcgaccagcg
     1261 tgccgagttc gggcgcggcg ccgggcgctg ccgcacgtgg cgccgccgcg ggtgtcggcg
     1321 gcggcgccgc caccggagca ctcggtgcct cgcagcgccg gaaccgcgac gtcatcaacg
     1381 ccttgccgag cgccgcggcg accttcgcgt cgccttcgag ccgaatgtag gggccgccct
     1441 tgtcggcgta gaccatgaac agcagttgca gcccgccgcg cacttcgccc atcagcgcca
     1501 cctggaaatc cttcggcggc gggctgttgc cgaagtagct gtacgaactc atggcgttgc
     1561 ggccggtcat gcgctggttg ccctgctcga ccagcagcgc gtcggcgtcg acgcgcaggc
     1621 gcggcgcggc cgcacttgcg cagtcgaccg catagcggcc gccgaactgg cgcagcgtcg
     1681 gtgcgtccag cccggtctgt gcggccgcgc tcgccgcgac cagcagggct gccgccagcg
     1741 cggcgctgcg gcccgaggcc ggctgatgat gactcttcat ggtcgtcccc cctgcccgtg
     1801 acatcgagca gtgcgaagcg tagcgtgcat ttcgttgcga aacaccggcg cgcccggcaa
     1861 ggttcgataa tcgggtcttt cccccgcctc aacgccgccg ccgccatgtt cgaacgagtg
     1921 ctcgccggga tcgtcctcgc cgtctgcgcg ctgctgctgc tgcgtctgac gatgagcgtg
     1981 ccgcggcgct atcgcttcga cgccgcatgg cgccgcatct ggctcgcgct gcgccgcgcc
     2041 ggcctgcgcg cgtggcactg gcgcgcggcg cgacgcgacg cgcggcgcgc ccgcgaggtg
     2101 gccgacgagg cgatccggcg cgcccgcgac cggggcgact gggacggcaa cgtctacacg
     2161 ccgaagtcgt tccgcaagcc gccgcgggac aagatgcatt gaagcgcccg tcgcctgcga
     2221 accccgagcg ctggtcggcc gactcgggcc ctcgcgatgt ggtgcggctc gacatcccgg
     2281 ccgatgcgct gcgcgagcgc acgttcgaga tcttcgtgcg cctggcggtc gccaacaagg
     2341 cgcatcgaac cgacgcgacg catgcactgc gcgtgctcgt cgacggcgtg ctcgaatggt
     2401 cgcgcagcgc ggccacgcac gtcgacggca gcgactcgct cgacctgcgc ctgcgccgca
     2461 gcgtgccgcc gggccggccg ttgcgcctca gcgcgagctg cgaactgcac ggcgcgcagc
     2521 gcgtgcgcct gtcgatcgac gccgaagagg aaggctgaag aagcctactt catcagcagg
     2581 tgcggcagcc aggtcgacag cggcggccag taggtcacga tgacgaggaa caccagcatc
     2641 gtcgcgagcc acggccagac cgcgaccgtc agctcgctga tgcccatctt cgtgatgccc
     2701 gacgccacgt acaggttcag gcccaccggc gggtggcaca tgccgacctc catgttgacg
     2761 acgatcagga tgccgaagtg gatcgggtcg atgcccagct tcatcgccac cgggaacagg
     2821 atcggcgcca tgatcaggat gatcgagctc ggctccatca cgttgccggc gagcagcagc
     2881 aggatgttga caacgagcag gaacgcgacg ggcccgaagc cctgcgcgat catccagtcc
     2941 gccatcgcct gcgggatgtt ctcgctcgtc atcaggaagc tgaacagcac cgcgttggtg
     3001 atgatgtaca gcagcatcgc gctcatgctg gcggagtcga gcagcacctt cgggatgcgc
     3061 ttgagcggca tgtccttgta gatgaacacc gccacgatga aggcgtacac cgccgccatc
     3121 gccgccgcct cggtgggcgt gaagatgccg gtgtagatgc cgcccatcac gacgacgatc
     3181 agcagcaggc cccagacgct ctcgcggaac gccgtccagc gctcgcccca gctcgcccgc
     3241 ggcatgcgcg ggtaatcgtt cttgcgggcg atgaaccagg tcgtcaggcc gagcgcggtc
     3301 gccatcgcga tgcccggcag gatgccggcg atgaacatcg cgccgaccga gacgttggtc
     3361 gagaccgagt acatcaccat cgcgatcgac ggcgggatca ggatgcccaa ggcgcccgag
     3421 gtggtgacga tgccggcacc gaagttgcgc gggaagccct gcttgaccat cgccggcagg
     3481 atcaccgagc cgatcgccac cacgcaggcc ggcgacgagc ccgacaccgc cgcgaacagc
     3541 gcgcaggcca tcacgccggc cagccccagg ccgccgtagt agtggccgat catcgaggtg
     3601 gcgaagcgga tcatccgtcg cgccacgccg ccgtgggtga ggaagttgcc ggccaggatg
     3661 aagaacggga tcgccatgat ctcgaacttc tcgatgccgg tgaacagctt cagcgcgacc
     3721 gacgcgatcg gcacgtcggt catcgtgaac aggaaggtca gcaccgtcag gccgagcgag
     3781 atgctgatcg gcatgcccgt gagcatcagc acgatcagca ggacgaagat gatggcggcg
     3841 ttcatttgcg gtctccgtgc gcgagttcct ggtccgacgg cacggccacc gccgcgacgt
     3901 cctcgatgcc ttcgacgtga ccgtggtcgt ggtgcggcag ctcaccggtg cgcgaaaagc
     3961 tccaggcgac ctgcaggaac cggtagcaca tcagcgaggt gcccagcggc acgcacaggt
     4021 agacgatcca catcggcatc tcgaggtcgg ccg
//
LOCUS       DATMJB010000593         2474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_499296, whole genome shotgun sequence.
ACCESSION   DATMJB010000593 DATMJB010000000
VERSION     DATMJB010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499296"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VNU48_12600"
     CDS             <1..756
                     /locus_tag="VNU48_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI12164.1"
                     /translation="SNAIRYTNDGTVLVGCRPRGDRVLLQVWDTGLGISEAEQARIFD
                     EFYQVPNTPKVSPDQRKGLGLGLAIVKRLADLMGASLTLRSTPGRGTVFTLELPAGRA
                     PRRSALLSVPGKGPVDLTLAGRRVVVVEDEPAVRAGLEVLLAGWGAQIDAFDSVGASR
                     AWAERSDPVLMKPDLLIVDYRLEEGLSGVDAIKALRARFGPALPAILVTGSTMTGHDR
                     EATEHNFHLLIKPVVPNKLRAMIAFKLGVKPGG"
     gene            complement(823..1311)
                     /locus_tag="VNU48_12605"
     CDS             complement(823..1311)
                     /locus_tag="VNU48_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12165.1"
                     /translation="MGHVPTDARPVASRAIGPATQLTYSGPSSNRRGLAMPIRSASLN
                     KQIQKNGARLHLAMAIRVHSEGADRAPSEHEQALRDERRFLKEAKTKRQLQAKLPKPK
                     AAWLAKPPKPPKAPKAAKEPKEPKEAKATKAHKPSRADKLAKKAENLDAAKKAAKKSA
                     KP"
     gene            1385..2059
                     /locus_tag="VNU48_12610"
     CDS             1385..2059
                     /locus_tag="VNU48_12610"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWI12166.1"
                     /translation="MKLYYHPISTTSRPIMLFAAESGIPLEMQVVDLFTGEHVRPPYA
                     ALNPNRLVPTLQDGDFILTENSAILKYLADKIDSPAYPKDLRQRARVNERMDWTSTQL
                     CIDLVYALVYPQIFDAHKRRSDEAQSATLERGKARAQAWLKVLDEHVLGTGNAYLCGD
                     AITIADYHAASYVALAEVIGSDLSGYPNVKRWLARMKALKSWPQVYEVIDGFAASLKD
                     KAMVAV"
     gene            2111..>2474
                     /locus_tag="VNU48_12615"
     CDS             2111..>2474
                     /locus_tag="VNU48_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI12167.1"
                     /translation="MIRKLHHNAYRCRDSEETRAFYEDFLGLPLVGTLEIGQSKTGRA
                     TQTLHTFYRLDDGSHLAFFESKEMPFEFKPQHDFDLHIALEVGAEVLQPMLAKGREWH
                     IEVRGPSDHGMIDSIYFRD"
BASE COUNT          382 a          859 c          815 g          418 t
ORIGIN      
        1 tcgaacgcga tccgctacac caacgacggc accgtgctgg tcggctgccg cccgcgcggc
       61 gaccgggtgc tgctgcaggt ctgggacacc gggctgggca tcagcgaagc cgagcaggca
      121 cgcatcttcg acgagttcta ccaggtgccg aacacgccca aggtcagccc ggaccagcgc
      181 aagggcctgg gcctcgggct ggcgatcgtc aagcgcctgg ccgacctgat gggcgcctcg
      241 ctgaccttgc gctccacgcc cggtcgcggc acggtgttca cgctcgagct gcccgcgggc
      301 cgggcgccgc gacgctcggc gctgctgtcg gtgccgggca aggggccggt cgacctgacg
      361 ctggcagggc gccgcgtcgt cgtcgtcgag gacgagccgg cggtgcgcgc cggcctcgag
      421 gtgctgctgg ccggctgggg cgcgcagatc gacgcgttcg acagcgttgg cgcgagccgc
      481 gcgtgggccg agcgcagcga cccggtgctg atgaagcccg acctgctgat cgtcgactac
      541 cggctcgagg agggcctgag cggcgtcgat gcgatcaagg cgctgcgcgc gcgcttcggc
      601 cccgcgctgc cggcgatcct ggtgaccggc agcaccatga ccggccacga ccgcgaggcg
      661 accgagcaca acttccacct gctgatcaag ccggtcgtgc cgaacaagct gcgcgcgatg
      721 atcgcgttca agctgggcgt gaagccgggc gggtgagggg cagtgcgagc cccgtcgctt
      781 cggcccccgg cgcgctcgcg gaacgacgtt ttttttcgcg cttcagggct tggctgactt
      841 cttcgccgct ttctttgcgg cgtcgaggtt ctcggccttc ttcgccagct tgtcggcgcg
      901 cgacggcttg tgagccttcg tcgccttcgc ctccttcggt tccttcggtt ccttcgcggc
      961 cttcggcgcc ttcgggggct tgggcggttt cgccagccag gcggccttgg gcttgggcag
     1021 cttggcctgc agctgccgct tggtcttggc ctccttcagg aaccggcgtt cgtcgcgcag
     1081 cgcctgttca tgctcggacg gcgctcgatc ggccccttcc gaatgaacgc ggatcgccat
     1141 cgccagatga agcctcgcgc cgttcttctg gatctgcttg ttcagtgacg cacttcgaat
     1201 cggcatggcg agccctctgc ggttggacga ggggccactg taggtcagtt gggtcgccgg
     1261 accaatcgcc ctactggcga ctggtcgggc gtccgtcggc acgtgaccca tgcggttgtc
     1321 gtggacggca gcggcgcggg cgtctatcgt tcgtctcgcc acaggcccga aaggagacag
     1381 caccgtgaag ctctactacc acccgatctc gaccaccagc cgccccatca tgctgtttgc
     1441 cgccgaaagc ggcattccgc tggagatgca ggtggtggac ctgttcaccg gcgagcatgt
     1501 gcggccgccc tacgcggcac tgaacccgaa caggctggtg cccacgctgc aggacggcga
     1561 cttcatcctc accgagaact cggcgatcct gaagtatctg gccgacaaga tcgactcgcc
     1621 cgcctacccc aaggatctgc gccaacgcgc gcgcgtcaac gagcgcatgg actggaccag
     1681 cacccagctc tgcatcgatc tggtctatgc cctggtctac ccgcagatct tcgacgccca
     1741 caagcgccgc agcgacgaag cgcagagcgc cacgctggag cgcggcaagg cgcgtgcgca
     1801 ggcctggctg aaggtgctcg acgagcacgt tctcggcacc ggcaatgcct acctctgcgg
     1861 cgacgcgatc accatcgccg actaccacgc cgcgtcgtac gtcgcgctgg ccgaggtgat
     1921 cggcagcgac ctgtcgggct atcccaacgt caagcgatgg ctcgcgcgca tgaaggcact
     1981 gaagagctgg ccgcaggtct acgaggtgat cgacggcttc gccgcctcgc tgaaagacaa
     2041 ggccatggtc gcggtctgat cacgatcttt ccccgatcgc ttcagcgcca ccccacccgg
     2101 agaactcgcc atgatccgaa agttgcacca caacgcctac cgctgccgtg actcggaaga
     2161 gacccgcgcg ttctacgagg acttcctcgg cctgccgctg gtcggaacgc tcgagatcgg
     2221 ccagagcaag accgggcgcg cgacgcagac gctgcacacc ttctaccggc tcgacgacgg
     2281 ctcgcacctg gcgttcttcg agtcgaagga aatgccgttc gagttcaagc cgcagcacga
     2341 cttcgacctg cacatcgcgc tcgaagtcgg cgccgaggtg ctgcagccga tgctggccaa
     2401 gggccgcgaa tggcacatcg aggtccgcgg cccgtccgac cacggcatga tcgattcgat
     2461 ctacttccgc gacc
//
LOCUS       DATMJB010000594         5775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_854405, whole genome shotgun sequence.
ACCESSION   DATMJB010000594 DATMJB010000000
VERSION     DATMJB010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854405"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1656)
                     /gene="gcl"
                     /locus_tag="VNU48_12620"
     CDS             complement(<1..1656)
                     /gene="gcl"
                     /locus_tag="VNU48_12620"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HWI12168.1"
                     /translation="MARMKAVMAAVLVMEKEGISQAFGVPGAAINPLYACLRERGGIA
                     HVLARHVEGASHMAEGYTRAKAGNIGVCIGTSGPAGTDMITGLYSAIADSIPILCITG
                     QAPRARLYKEDFQAVDIESIAKPVTKWSVTVREPAQVPRAFQQAFHLMRSGRPGPVLI
                     DLPFDVQMAEIEFDIDTYGPLPVYKPQATRKQIEKALAMLQASERPLIVAGGGIINAD
                     ASALLQEFAELTNTPVIPTLMGWGTIPDDHPLMAGMCGLQTSHRYGNATMLASDFVLG
                     IGNRWANRHTGSPEVYCKGRSFVHVDIEPTQIGRVFAPELGIVSDAKAALQLFVEVAR
                     EMRAAGKLKDRSSWVAECRERKRTLLRKTNLDTVPMKPQRVYQCMNNNFERGTTYVST
                     IGLSQIAGAQFLHVFGPRQWINCGQAGPLGWTIPAALGVRVADPTRKIVALSGDYDFQ
                     FMIEELAVGAQFKLPYIHMVVNNSYLGLIRQAQRGFDMDYCVQLGFDNINAPQMEGYG
                     VDHVAVAEGLGCKAIRVRSPNEFQHAFDQARELMREFEVPVVVE"
     gene            1789..2715
                     /locus_tag="VNU48_12625"
     CDS             1789..2715
                     /locus_tag="VNU48_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWI12169.1"
                     /translation="MDRITGVQLFIRIVETGSFSKASVDLGVTQPTATKHVAAVEARL
                     GARLLNRNTRGVSATEIGALYYEKCKAIQRELEEADNLASLLQSRVGGQLRISTSVGF
                     GRRVLTPLVLAFMRQHPDISIDLSFDDRYVNLVEQGVDVAIRMGRLADSSLGARYLGQ
                     NPWVLVAAPAYLQAHPAPLAPADVAEHPCLIYSSVQGDERWHFSGGGDGSGREHSVPV
                     HGPLRSNNLSVLLEAAVGGMGLAILPWYVAHSAVQAGQATALLTDYALPVQEMHAVFP
                     SPKLLPSKVSSFIAWLQQHFTPEWWKGVAVPN"
     gene            complement(2819..4129)
                     /locus_tag="VNU48_12630"
     CDS             complement(2819..4129)
                     /locus_tag="VNU48_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctA"
                     /protein_id="HWI12170.1"
                     /translation="MPRFLRSLFGQVVLALVVGVLLGLAMPEFAIKLKPLGDGFIKLI
                     KMIIPLLVFCVVVHGIAGAGDLKRVGRVGVKALVYFEVVTSIALLLGLVLAFVFHPGA
                     GMNVDPGTLDAKAMSAYSENASKLTGGGTVEFLMKLVPTTVGDAFARGDVLQVLLFAI
                     LFGCALALIGERGKAVAHFIDDLSHVIFKIMGILIKLAPLGVLGAIAFTVGQYGIGSL
                     KQLGMLVVLFYVAVLIFVFVVLGTILRLCGFSIVKLLRYLREELTIVFATTSSDSVLP
                     QIMAKLKYLGIRDSTVGLVIPTGYSFNLDAFSIYITLAAVFIAQATNTPISMTDLLTI
                     LAISLVTSKGAHGVPGSAIVVLAATLHAIPAIPAIGLVLVLSVDWFMGIARALGNLIG
                     NCVATVVVAAWEGDIDRERAHAVLDGRPVVDDAEAAVLAVAVAH"
     gene            complement(4328..5713)
                     /locus_tag="VNU48_12635"
     CDS             complement(4328..5713)
                     /locus_tag="VNU48_12635"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HWI12171.1"
                     /translation="MPTLLIRHARLLVTMDAQRREIADGAVFVRDHVIEAVGPSAELP
                     ATADEVIDARDQVVIPGLVNTHHHMFQTLTRARVQDHELFGWLRGLYPIWANLTPEMV
                     KVSTQTAMAELLLSGCTTSSDHLYVYPNGITLEDSIEGASEIGMRFHAARGSMSVGES
                     QGGLPPDHVVESESAILKETQRAIERWHDPGRFAMQRVVVAPCSPFSVSRELMRDSAL
                     LARSFGASHGVSLHTHLAENDFDVAYTREKFGCTPAEYVEALGWVGPDVWCAHCVKLD
                     AAGIALFARSGTGVAHCPCSNMRLASGIAPVRALRDAGANVAIGVDGAASNDAGHLLG
                     EVRMAMLLQRVAHGPVNGPSAMSAREALEIATLGGAKVLGRDDIGALAVGMAADIVTV
                     PLDEIGMAGAQHDPLAALLFCQAPRVRHAIVNGRVVVRDGRLATLELPVLIERHNALA
                     AQLAQAAHSTH"
BASE COUNT          896 a         2000 c         1997 g          882 t
ORIGIN      
        1 ctcgacgacg accggcacct cgaactcgcg catcaattcg cgcgcctggt cgaaagcgtg
       61 ctgaaactcg ttcgggctgc gcacgcggat cgccttgcag ccgaggcctt cggcgaccgc
      121 gacgtggtcg acgccgtagc cttccatctg cggcgcgttg atgttgtcga agcccagctg
      181 cacgcagtag tccatgtcga agccgcgctg cgcctggcgg atcagcccga ggtacgagtt
      241 gttcacgacc atgtgaatgt agggcagctt gaactgcgcg ccgaccgcga gctcctcgat
      301 catgaactgg aagtcgtagt cgcccgagag cgcaacgatc ttgcgcgtcg ggtcggcgac
      361 acgcacgccc agcgccgcgg ggatggtcca gcccagcggg ccggcctggc cgcagttgat
      421 ccactggcgc gggccgaaca cgtgcaggaa ctgcgcgccc gcgatctgcg agaggccgat
      481 cgtgctgaca taggtcgtgc cgcgctcgaa attgttgttc atgcactggt agacgcgctg
      541 cggcttcatc ggcacggtgt cgagattggt cttgcgcagc agcgtgcgct tgcgctcgcg
      601 gcattcggca acccagctgc tccggtcctt cagcttgccg gcggcgcgca tttcgcgtgc
      661 gacctcgacg aagagctgca gcgccgcctt cgcgtccgag acgataccga gctcgggcgc
      721 gaagacgcgg ccgatctggg tcggctcgat gtcgacgtgc acgaagctgc ggcccttgca
      781 atacacctcg ggcgagccgg tgtggcggtt cgcccagcgg ttgccgatgc cgagcacgaa
      841 gtcgctcgcg agcatggtcg cgttgccgta gcggtgcgag gtctgcagcc cgcacatgcc
      901 ggccatcagc gggtggtcgt cggggatggt gccccagccc atcagggtcg gaatcaccgg
      961 cgtgttggtc agttcggcga actcctgcag cagcgcgctc gcgtcggcgt tgatgatgcc
     1021 gccgccggcg acgatcaacg ggcgctcgct ggcttgcagc atcgcgagcg ccttctcgat
     1081 ctgcttgcgc gtggcctgcg gcttgtagac cggcagcggc ccgtaggtgt cgatgtcgaa
     1141 ctcgatctcg gccatctgca cgtcgaatgg caggtcgatc agcaccgggc ccgggcggcc
     1201 cgagcgcatc aggtggaagg cctgctggaa cgcacgcggc acctgcgcgg gctcgcgcac
     1261 cgtgacgctc cacttcgtca ccggcttggc gatcgactcg atgtcgaccg cctggaaatc
     1321 ttccttgtac agccgcgcgc gcggtgcctg gccggtgatg cacaggatcg ggatgctgtc
     1381 ggcgatcgcc gaatacagcc cggtgatcat gtcggtgccg gccgggccgg aggtgccgat
     1441 gcacacgccg atgttgccgg ccttggcgcg cgtgtagccc tcggccatgt gcgaggcgcc
     1501 ctcgacgtgg cgcgccagca cgtgcgcgat tccgccgcgc tcgcgcaggc acgcgtacag
     1561 cgggttgatc gcggcgcccg gcacgccgaa ggcctgcgag atgccttcct tctccatcac
     1621 caaaacggcg gccatcacgg ctttcatgcg tgccattgct cagtctcctt gatcgttgat
     1681 cgaacgttgt ccgcatgatc gccagagcgc gccgcgaagg caacggcgcg tgccgataag
     1741 tgctattccg cggcggaatg ggcgccccgc caaggcatga tgccgcccat ggaccgcatc
     1801 accggcgtac agctcttcat ccgcatcgtc gagaccggca gcttctcgaa ggcctcggtc
     1861 gacctcggcg tgacccagcc gaccgcgacc aagcacgtcg ccgcggtcga ggcgcgcctg
     1921 ggcgcgcggc tgctgaaccg caacacgcgc ggcgtcagcg cgaccgagat cggcgcgctg
     1981 tactacgaga agtgcaaggc gatccagcgc gagctcgagg aggccgacaa cctcgcctcg
     2041 ctgctgcagt cgcgcgtcgg cgggcagctg cgcatcagca cctcggtggg cttcggccgt
     2101 cgcgtgctga cgccgctggt gctggccttc atgcgccagc acccggacat ctcgatcgac
     2161 ctgagcttcg acgaccgcta cgtcaacctc gtcgagcagg gcgtggacgt cgcgatccgc
     2221 atggggcgtc tcgcggattc atcgctgggc gcgcgctacc tcgggcagaa cccctgggtg
     2281 ctggtggccg cgccggccta tctgcaggcg catccggcgc cgctcgcgcc ggccgatgtg
     2341 gccgaacacc cttgcttgat ctacagcagc gtgcagggcg acgagcgctg gcacttcagt
     2401 ggcggcggcg acggcagcgg gcgcgagcat tcggtgccgg tgcacggccc gctgcgctcg
     2461 aacaacctgt cggtgctgct cgaggccgcg gtcggcggca tggggctggc gatcctgccg
     2521 tggtacgtgg cccacagcgc ggtccaggcc ggccaggcga cggcgctgtt gaccgactac
     2581 gcgctgccgg tgcaggagat gcacgcggtg ttcccctcgc cgaagctgct gccgtccaag
     2641 gtcagcagct tcatcgcctg gctgcagcag cacttcactc ccgagtggtg gaagggcgtg
     2701 gccgtgccga actgaatgcc ggcagcggcg ccagcgtggc gcgcagccgc tccagcgggt
     2761 cgcgcgacgc cgacgattgc ccgagcgcgt ttttgcgcga tctcgatctg ggtgctgctc
     2821 agtgggccac cgcgaccgcg agcaccgcgg cctcggcgtc gtcgaccacc ggccggccgt
     2881 cgagcaccgc gtgcgcgcgc tcgcggtcga tgtcgccttc ccaggccgcc accaccacgg
     2941 tcgcgacgca gttgccgatc aggttgccca aagcgcgggc gatgcccatg aaccagtcga
     3001 ccgacagcac cagcaccagg ccgatcgccg ggatcgcggg aatggcgtgc agcgtcgccg
     3061 cgagcacgac gatcgccgaa cccggcacgc cgtgcgcgcc cttcgaggtg acgagcgaga
     3121 tcgccaggat cgtcagcagg tcggtcatcg agatcggcgt gttggtggcc tgcgcgatga
     3181 acaccgcggc cagcgtgatg tagatcgaga acgcgtcgag gttgaacgag tagccggtcg
     3241 ggatcaccag gccgacggtc gaatcgcgga tccccaggta cttgagcttg gccatgatct
     3301 gcggcagcac gctgtcggac gaggtggtcg cgaacacgat cgtcagctcc tcgcgcaggt
     3361 agcgcagcag cttgacgatc gagaagccgc acaggcgcag gatcgtgccc aggaccacga
     3421 acacgaagat cagcaccgcg acatagaaca gcaccaccag catgccgagc tgcttcagcg
     3481 agccgatgcc gtactggccg accgtgaacg cgatcgcgcc gagcacgccc aacggcgcca
     3541 gcttgatcag gatgcccatg atcttgaaga tgacgtgcga caggtcgtcg atgaagtgcg
     3601 ccaccgcctt gccgcgctcg ccgatcagcg ccagcgcgca gccgaacagg atcgcgaaca
     3661 gcagcacctg cagcacgtcg cccctcgcga acgcgtcgcc gacggtggtc ggcaccagct
     3721 tcatcaggaa ctcgaccgtg ccgccgccgg tgagcttgct ggcgttctcg ctgtaggcgc
     3781 tcatcgcctt cgcgtccagc gtgccggggt cgacgttcat gcccgcgccg gggtggaaga
     3841 cgaaggccag caccagcccg agcagcagcg cgatgctggt cacgacctcg aagtacacca
     3901 gcgccttgac gccgacccgg cccacgcgct tcaggtcgcc ggcgccggcg atgccgtgca
     3961 cgacgacgca gaacacgagc agcgggatga tcatcttgat cagcttgatg aagccgtcgc
     4021 cgagcggctt gagcttgatc gcgaactcgg gcatcgccag gccgagcagc acgccgacga
     4081 cgagcgccag cacgacctgg ccgaacaggg aacgcaggaa acggggcatg agagtttctc
     4141 cgacgggtgg ggcaagcgtc tttgacgaca cgcttgcata caagcaacgc agactgcgac
     4201 gcgacgttca atgggggcga tttccccgcg tccgattcgc aagtcccggg ccagcacggc
     4261 acgcggctga gcgccgagac gcaccggccg ggtgcgcctc ggcctttggc catcgtgcct
     4321 tgcccgatca atgcgtcgag tgcgccgcct gcgcgagctg tgcggccagc gcgttgtggc
     4381 gctcgatcag caccggcagc tcgagcgtcg cgagccgccc gtcgcgcacc acgacccggc
     4441 cgttgacgat cgcgtgccgc acccgcggcg cctggcagaa cagcagcgcg gcgagcggat
     4501 cgtgctgcgc gccggccatg ccgatctcgt cgagcgggac ggtgacgatg tcggcggcca
     4561 tgccgacggc gagcgcgccg atgtcgtcgc gacccagcac cttcgcgccg ccgagcgtcg
     4621 cgatctcgag cgcctcgcgc gcgctcatcg ccgacgggcc gttcacgggg ccgtgcgcca
     4681 ccctttgcag cagcatcgcc atgcgaacct cgccgagtag gtggccggcg tcgttcgagg
     4741 ccgcgccgtc gacgccgatg gccacgttcg cgccggcatc gcgcagcgcg cggaccggcg
     4801 cgatgcccga ggccagccgc atgttcgagc acgggcagtg cgcgacgccg gtgccgctgc
     4861 gcgcgaacag cgcgatgccg gcggcgtcga gcttgacgca gtgcgcgcac cagacgtccg
     4921 gcccgaccca gccgagcgcc tcgacgtact cggccggcgt gcagccgaac ttctcgcgcg
     4981 tgtaggcgac atcaaagtcg ttctcggcca ggtgcgtgtg cagcgacacg ccgtgagacg
     5041 cgccgaaact tcgggcgagc agcgccgaat cgcgcatcag ctcgcggctc accgagaacg
     5101 gcgagcacgg tgcgacgacg acgcgctgca tcgcgaagcg gcccgggtcg tgccagcgct
     5161 cgatcgcgcg ctgcgtctcc ttcaggatcg cgctctcgct ctcgaccacg tggtccggcg
     5221 gcagcccgcc ctggctctcg ccgacgctca tcgagccccg cgcggcgtgg aagcgcatgc
     5281 cgatctcgct ggcgccctcg atgctgtctt cgagcgtgat gccgtttggg tagacgtaga
     5341 gatggtcgct gctggtcgtg cagcccgaca gcagcagctc ggccatcgcg gtctgcgtcg
     5401 agaccttcac catctccggc gtcaggttcg cccagatcgg gtacaggccg cgcagccagc
     5461 cgaacagctc gtggtcctgc acgcgcgccc gggtcagcgt ctggaacatg tggtggtgcg
     5521 tgttgaccag gccggggatc acgacctggt cgcgcgcgtc gatcacctcg tcggccgtgg
     5581 ccggcagctc ggcgctcgga ccgaccgcct cgatcacgtg gtcgcgcacg aacaccgcgc
     5641 cgtcggcgat ctcgcgccgc tgcgcgtcca tcgtgacgag cagtcgcgcg tggcggatca
     5701 gcagcgtggg catggcgcgg gcggctcggc ggggcgcagc ggcggcgtcc gcttcacttc
     5761 gccgcggcgc cggcg
//
LOCUS       DATMJB010000595         2748 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_262169, whole genome shotgun sequence.
ACCESSION   DATMJB010000595 DATMJB010000000
VERSION     DATMJB010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262169"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1210
                     /locus_tag="VNU48_12640"
     CDS             245..1210
                     /locus_tag="VNU48_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006412508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HWI12172.1"
                     /translation="MLAAFVPGAWAQAPIVIKFSHVVTPDTPKGKAALRFKQLAEAAS
                     QGRVKVEVYPNSQLYKDKEELEALQLGAVQMLAPSLSKFGPLGAKQFEMFDLPYIFQT
                     EEQFVQVTRGKIGQSLLASLESKSIKGLAFWSGGFHVFSANKLLRKPDDLKGLKMRIP
                     SSKVLEASLRTMGALPQVMPFSEVYQAMQSGVVDGTENILSSYTSQKYYEVQKHIVLT
                     NHTHTGYALIVNKKFWDGLAPDLQAVLNTAVKQATDYEEQLVAQENKDALAELEASGR
                     TQVQDLTPAEREVWRRTLWPVHKEMESRIGAALLAQVYEATGTAK"
     gene            1273..2289
                     /locus_tag="VNU48_12645"
     CDS             1273..2289
                     /locus_tag="VNU48_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HWI12173.1"
                     /translation="MTSTTRRLALAVAAALAFGAPAASFAQAPIIIKFSHVVAPDTPK
                     GNGALKFKELAEERTKGKVKVEVYPNSQLYKDKEELEALQLGSVQMLAPSLAKFGPLG
                     VKEFEVFDLPFIFKDAAAFKAVTEGPVGTGLFAKLEPKGIHGLAFWDNGFHIMSANKP
                     LHHVADFKGLKMRIQSSKVLEAQMRALGAIPQVMAFSELYSGLQSGVVDGTEGVPSNF
                     FTQKIFEVQKDMTISNHGHLAYAVIVNKKFWDGLPADIRTTLEGAMRDATTYANAIAA
                     TENATALDKIKASGKTKVYTLSADEANEWKKALMPVHKEMEARVGKPTIDAVYKAAGF
                     VAPK"
     gene            2346..>2748
                     /locus_tag="VNU48_12650"
     CDS             2346..>2748
                     /locus_tag="VNU48_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HWI12174.1"
                     /translation="MFNRILNHLEEWLITFLIGAATLVIFFAVAHRFLSGVPWIQDYT
                     VRLNVSWAQELCIYMFVWMAKFGAAYGVRTGIHVGVDVIIRKLTGTPQKALVMFGLLA
                     GALFTGVVATLGANFVWHMSQTDQVSADLEID"
BASE COUNT          537 a          902 c          886 g          423 t
ORIGIN      
        1 atctgtctcg cggctggcgc gggcccgcga ggtccacgag gtaggcctct tcgcggtcca
       61 tggcggcccg gcgctcggcc cgccgggcac gcgcgcatga agccacaaat gtggaactcc
      121 acaatgccgc gccggcgccg gcggccccag aatcgcggcg aagaaccaat tccaagaaca
      181 ccggagacaa ggcttgtcga agtccatcgt tcgtcgaacg gcgtgcgccg tcggcgcatg
      241 cgcgctgctc gcggcgttcg tgccgggcgc ctgggcacag gcgccgatcg tcatcaagtt
      301 cagccacgtc gtcacgcccg acacgcccaa gggcaaggcc gcgctgcgct tcaagcagct
      361 cgccgaagcg gccagccaag gccgcgtcaa ggtcgaggtc taccccaaca gccagctcta
      421 caaggacaag gaagagctcg aggcgctgca actcggcgcg gtgcagatgc tcgcgccgtc
      481 gctgtcgaag ttcggcccgc tgggggcgaa gcagttcgag atgttcgacc tgccctacat
      541 cttccagacc gaggagcagt tcgtccaggt cacgcgcggc aagatcggcc agtcgctgct
      601 ggccagcctg gaaagcaaga gcatcaaggg cctggccttc tggagcggcg gctttcatgt
      661 gttctcggcc aacaagctgc tgcgcaagcc cgacgacctg aagggcctga agatgcgcat
      721 cccgtcgagc aaggtgctcg aggcctcgct gcgcacgatg ggcgcgctgc cgcaggtgat
      781 gccgttctcc gaggtctacc aggcgatgca gtcgggtgtg gtcgacggca ccgagaacat
      841 cctgtccagc tacacctcgc agaagtacta cgaggtgcag aagcacatcg tgctgaccaa
      901 ccacacccac accggctatg cgctgatcgt caacaagaag ttctgggacg gcctggcgcc
      961 ggacctgcag gcggtgctga acacggccgt caagcaggcc accgattacg aagagcagct
     1021 tgtcgcgcag gagaataagg acgcgctggc cgagctcgag gcttcgggca ggacgcaggt
     1081 acaggacctc acgccggccg agcgcgaggt ctggcgcagg accttgtggc cggtgcacaa
     1141 ggaaatggaa tcgcgcatcg gcgcggcgct gctcgcacaa gtctacgaag ccaccggcac
     1201 ggccaagtag cggtgctggc cctcaatcac gttcgttccg ttctcatcat cattcttcgc
     1261 aggagacttc ccatgacctc gaccacccgc cgcctcgcgc tcgccgtcgc tgccgcgctc
     1321 gccttcggag cgcccgctgc cagcttcgcg caggccccga tcatcatcaa gttcagccac
     1381 gtcgtcgcgc ccgacacgcc caagggcaac ggtgcgttga agttcaagga actcgccgag
     1441 gagcgcacca agggcaaggt caaggtcgag gtctacccga acagccagct ctacaaggac
     1501 aaggaagagc tcgaggcgct gcagctcggc tcggtgcaga tgctggcacc gtcgctggcc
     1561 aagttcgggc cgctgggcgt gaaggaattc gaggtcttcg acctgccgtt catcttcaag
     1621 gatgcggcgg cgttcaaggc cgtcaccgaa ggtcccgtcg gcaccggcct gttcgccaag
     1681 ctcgagccca agggcatcca cggcctggcc ttctgggaca acggctttca catcatgagc
     1741 gcgaacaagc cgctgcacca cgtcgccgac ttcaagggcc tgaagatgcg gatccagtcg
     1801 tccaaggtgc tggaagcgca gatgcgcgcg ctcggcgcga tcccgcaggt gatggcgttc
     1861 agcgagctgt actcggggct gcagtcgggg gtggtcgacg gcaccgaggg cgtgccgtcg
     1921 aacttcttca cgcagaagat cttcgaggtc cagaaggaca tgacgatctc caaccacggt
     1981 cacctcgcgt acgcagtcat cgtcaacaag aagttctggg acgggctgcc ggccgacatc
     2041 cgcaccacgc tcgaaggcgc gatgagggac gcgacgacct acgccaacgc gatcgccgcg
     2101 accgagaacg cgaccgcgct cgacaagatc aaggccagcg gcaagaccaa ggtctacacg
     2161 ctgagcgccg acgaggccaa cgagtggaag aaggcgctga tgccggttca caaggaaatg
     2221 gaagcgcgcg tgggcaagcc gacgatcgac gctgtctaca aggcggccgg cttcgtcgcg
     2281 ccgaagtaag cgcgcggcgt cgcgcccgaa ccgcgcggcg accacaagaa aagcgggaga
     2341 cctgcatgtt caaccgaatc ctgaaccacc tggaggaatg gctcatcacc ttcctgatcg
     2401 gggcggccac gctggtgatc ttcttcgccg tggcgcaccg cttcctgtcc ggcgtgccct
     2461 ggatccagga ctacacggtc aggctcaacg tcagctgggc ccaggaactg tgcatctaca
     2521 tgttcgtctg gatggccaag ttcggcgccg cctacggcgt gcgcaccggc atccacgtcg
     2581 gcgtggacgt gatcatccgc aagctcaccg gcacgccgca gaaggcgctg gtgatgttcg
     2641 ggttgctggc gggcgcgctg ttcacaggcg tcgtcgccac gctcggcgcg aacttcgtct
     2701 ggcacatgtc gcagaccgat caggtctcgg ccgacctcga gatagatc
//
LOCUS       DATMJB010000596         3292 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_385630, whole genome shotgun sequence.
ACCESSION   DATMJB010000596 DATMJB010000000
VERSION     DATMJB010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385630"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VNU48_12655"
     CDS             complement(<1..800)
                     /locus_tag="VNU48_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWI12175.1"
                     /translation="MSTSPAPVDYQTHPERYKHWRLQFEGSVATLVMAIDEDGGLREG
                     YKLKLNSYDLGVDIELNDAVQRIRFEHPEVRSVIVTSGRDRVFCSGANIFMLGKSSHP
                     WKVNFCKFTNETRNGLEDSSRHSGLKFLAAVNGACAGGGYALAAACDEIILVDDRSSA
                     VSLPEVPLLGVLPGTGGLTRLTDKRKVRHDHADIFCTTSEGIRGKKAKEWKLVDEVVK
                     TQGFAQRVQERARELAAASDRPAGAKGVALTPLARTLTADALKYAHIEV"
     gene            complement(904..1806)
                     /locus_tag="VNU48_12660"
     CDS             complement(904..1806)
                     /locus_tag="VNU48_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWI12176.1"
                     /translation="MSTSDPSATELKDPFLAALGERTRALRARRGLTRKGLAKAADVS
                     ERHLANVEVGVGNATVQFLRQLAQALNCSLAELVGDETASSPEWLMIRELLRGRSEAE
                     LLQARGALAALFGAPASQAARRQRIALIGLRGAGKSTLGRALAEAWRVPFVELNRSIE
                     ALAGCTLSEIHSLYGPAAYRRYENRAMEETIQRHPRAVIATPGGIVSDPATFNLLLAH
                     CYTVWVRATPEEHMNRVLAQGDTRPMAGSNGDREAMSDLRRILESRAAFYSKADAVFD
                     TSGMSPDAALAGLRAQLQDIVTAS"
     gene            1877..>3292
                     /locus_tag="VNU48_12665"
     CDS             1877..>3292
                     /locus_tag="VNU48_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HWI12177.1"
                     /translation="MQDITSSPTASSPGLTPPPERFNAAAHLLALNAGRGPKLAYIDD
                     LHDLSYGELAERVRRCAAGLLALGVKREERVLMLMHDSVDWPVAFLGALYAGIVPVPV
                     NTLLSTDDYAYMVEHSAAQAVLVSRALWPVLQGAVAAAGRPVPVVVAGGGEAPAGTRR
                     FAALLEAAPLAAPADTRADDFGFWLYSSGSTGRPKGAVHTHANLYWTAALYGTPVLGL
                     HEADIGFSAAKLFFAYGLGNALTFPLSVGATTVLMAERPTPAAVFKRWVERGVTVFFG
                     APTGYAGMLASPDLPARAAVRLRLCSSAGEALPREIGERFTQHFGCEIVDGLGSTEML
                     HIFLSNRPGQVQYGSSGRVVPGYRLKLVDEHGGEAKPGEIGDLYVSGPSSALMYWGQR
                     DKSRATFLGEWTRTGDKYSIDDRGCYVYAGRSDDMLKVSGMYVSPFEVEATLMQHAAV
                     LECAVIGIADADGLTKTKAFVV"
BASE COUNT          464 a         1145 c         1155 g          528 t
ORIGIN      
        1 acctcgatgt gcgcgtactt cagcgcatcg gcggtcagcg tgcgcgccag cggcgtcagc
       61 gcaacgccct tcgcgccggc cggccggtcg ctcgccgcgg cgagttcgcg tgcacgctcc
      121 tgtacacgct gcgcgaaacc ctgcgtcttg acgacctcgt cgaccagctt ccattccttg
      181 gccttcttgc cgcggatgcc ttcgctggtc gtgcagaaga tgtcggcgtg gtcgtggcgc
      241 accttgcgct tgtcggtcag ccgggtcagg ccgccggtgc ccggcagcac gcccagcagc
      301 ggcacctcgg gcaggctgac cgccgacgag cggtcgtcga ccaggatgat ctcgtcgcag
      361 gccgcggcca gcgcgtagcc gccgccggcg catgcaccgt tgaccgcggc gaggaacttc
      421 aggcccgaat ggcggctgga gtcttcgagt ccgttgcgcg tctcgttcgt gaacttgcag
      481 aagttgacct tccacgggtg gctgctcttg ccgagcatga agatgttcgc gccggaacag
      541 aacacgcggt cgcggccgct ggtgacgatc acgctgcgca cctcggggtg ctcgaagcgg
      601 atgcgctgga ccgcgtcgtt cagctcgatg tccacgccca ggtcgtagct gttgagcttg
      661 agcttgtagc cctcgcgcag gccgccgtct tcgtcgatcg ccatcaccag cgtcgcgacc
      721 gacccttcga actgcagccg ccagtgcttg tagcgctcgg gatgggtctg gtagtcgacg
      781 ggggcgggtg aggtgctcat gagtggctcc aagtgacagg cagcatgctg catcgcgtgc
      841 ggatgaaatg caatatactg catcaactcg acatcggcaa gcccgatcgc caggcgctgc
      901 gagtcagctt gcggtgacga tgtcctgcag ttgcgcgcgc aggccggcca gcgccgcgtc
      961 cgggctcatc ccgctggtgt cgaacaccgc gtcggccttc gaatagaagg ccgcgcgact
     1021 ctcgaggatg cggcgcagat cgctcatcgc ctcgcggtcg ccgttgctgc cggccatcgg
     1081 ccgggtgtcg ccctgcgcca gtacgcggtt catgtgctct tcgggcgtgg cacgcaccca
     1141 cacggtgtag cagtgcgcca gcagcaggtt gaaggtggcg gggtcggaga cgatgccgcc
     1201 gggcgtggcg atcaccgcgc gcgggtggcg ctggatcgtc tcttccatcg cccgattctc
     1261 gtagcgccgg taggccgccg ggccgtacag cgagtggatc tcggacaagg tgcagccggc
     1321 gagcgcctcg atgctgcggt tcagctcgac gaagggcacg cgccaggcct cggcgagtgc
     1381 ccgcccgagc gtgctcttgc cggcgccgcg caggccgatg agcgcgatgc gctggcgccg
     1441 cgcggcctga ctcgccggcg cgccgaacag cgccgccagc gcgccgcgcg cctgcagcag
     1501 ctcggcctcg ctgcgcccgc gcagcagctc gcggatcatc agccactcgg gcgagctcgc
     1561 ggtctcgtcg ccgaccagtt cggcgagcga gcagttcagc gcctgcgcca gctgccgcag
     1621 gaactgcacc gtcgcgttgc cgacgccgac ctcgacattc gcgaggtgcc gctcggacac
     1681 gtcggccgcc ttcgccagcc ccttgcgcgt gagcccgcgg cgcgcccgca gcgcgcgcgt
     1741 gcgctcgccc agcgccgcga ggaatgggtc cttgagctcg gtggcggaag ggtcggatgt
     1801 ggacagatcc aagggtcgca gtcttcgggt ttgcgcggag atcgcgctgg agattcggcg
     1861 ctatagtgca caaaacatgc aagatattac atcgtcaccc acagcgtcaa gccccgggct
     1921 taccccgcca ccggaacgct tcaacgccgc ggcgcatctg ctggcgttga acgccgggcg
     1981 cgggcccaag ctcgcctaca tcgacgacct gcacgacctc agctacggcg aactcgccga
     2041 acgcgtgcgc cgctgcgccg ccgggttgct ggccctgggc gtgaagcgcg aggagcgcgt
     2101 gctgatgctg atgcacgaca gcgtcgactg gccggtcgcg ttcctcggcg cactgtatgc
     2161 cggcatcgtg ccggtgccgg tgaacacctt gctcagtacc gatgactacg cctacatggt
     2221 cgagcacagc gccgcgcagg ccgtgctggt ctcgcgggcc ctgtggccgg tgctgcaggg
     2281 cgcggtcgcc gcggccggcc ggcccgtgcc ggtcgtcgtc gccggtggcg gcgaagcgcc
     2341 ggcaggcacg cggcgcttcg ccgcgctgct cgaggccgca cccctggccg cacccgccga
     2401 cacgcgcgcc gacgacttcg gtttctggct ctattccagc ggctcgaccg ggcgcccgaa
     2461 aggcgcggtg cacacgcatg ccaacctgta ctggacggcc gcgctgtacg gcacgccggt
     2521 gctcggcctg cacgaggccg acatcggctt ctcggccgcg aagctgttct tcgcctacgg
     2581 cctcggcaac gcgctgacct tcccgctcag tgtcggcgcg accaccgtgc tgatggccga
     2641 gcggccgacg cccgcggcgg tgttcaagcg ctgggtcgag cgcggcgtca cggtcttctt
     2701 cggcgcgccg accggctacg ccggcatgct cgcatcgccg gacctgccgg cgcgcgccgc
     2761 ggtgagactg cggctgtgct catcggccgg cgaagcgttg ccgcgcgaga tcggcgagcg
     2821 cttcacgcag cacttcggct gcgagatcgt cgacgggctg ggctcgaccg agatgctgca
     2881 catcttcctg tcgaaccggc cgggtcaggt gcagtacggc agcagcggtc gcgtcgtgcc
     2941 cggctaccgg ctcaagctgg tcgacgagca cggcggcgag gcgaagcccg gcgagatcgg
     3001 cgacctgtac gtcagcggcc cgagttcggc gctgatgtac tggggccagc gcgacaagtc
     3061 gcgcgccacg ttcctgggcg agtggaccag gaccggcgac aagtactcga tcgacgaccg
     3121 cggctgctac gtgtatgcgg gccgcagcga cgacatgctc aaggtcagcg gcatgtatgt
     3181 ctcgccgttc gaggtcgagg ccacgctgat gcagcacgcg gcggtgctcg aatgcgcggt
     3241 gatcggcatc gccgacgccg acgggctgac caagaccaag gccttcgtcg tc
//
LOCUS       DATMJB010000597         2732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_273840, whole genome shotgun sequence.
ACCESSION   DATMJB010000597 DATMJB010000000
VERSION     DATMJB010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273840"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1750
                     /locus_tag="VNU48_12670"
     CDS             44..1750
                     /locus_tag="VNU48_12670"
                     /inference="COORDINATES: protein motif:HMM:NF025085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HWI12178.1"
                     /translation="MNRDPLDMIEIPADDCAFEVVDDVNPFFAPVATDLLDSLLGQYQ
                     AARRNIDALHGVVMGGEFGNVVHYFIEGNAGDDQLHRSLYVDKLFAIDGAVGALNSAF
                     WSKALALTDVYALMPQERKNQWNAQMRSPMGVKKRNRSYGSTASEWELEPLPDFDEAT
                     VRTTIAGLLHARSQFLAERVDGIFRNLSGHHVTNAPEAFGKRMIVARVLSEYDTINHD
                     RAGYLHDLRCVIAKFMGREEPAHFGTTDALIKSAKEQRGEWVYADGGALKLKVFKVGT
                     AHLEVHPDMAYRLNQILAFMHPHAIPAEFRARPARKSKAWALLRRPLPFAVTSLIATM
                     RPGAEKIPNDFRDGMRTVPNTRRFDFGDHDKAALAEAVRIIESIGGVKTGHYWTFDYE
                     PADVLREIATTGCIPDHVSHQFFPTPETVAAAVIDAAEIGDGDTCLEPSAGHGALADL
                     MPRDRTRCVEVSPLHCAILKAKGFDTIEADFIKYAEGSAERFDRVVGNPPFCDGRWQA
                     HTLAAAGLVAPGGRLVFVLPASARNALTLPGFDCAWSRMYVDEFAGTGAAVVILTAIR
                     KA"
     gene            1755..2357
                     /locus_tag="VNU48_12675"
     CDS             1755..2357
                     /locus_tag="VNU48_12675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12179.1"
                     /translation="MATHTLPPAGDSGPPIYYPPDLQLTLTGAEVCPCSELHSTHLTD
                     WSLSFGAIDARNAVPTGSVRGIGSAAVFGIDFCVRFSEPMATLCEVPAAAPAPSPPDP
                     PRVVEPPLSPAERRALQRRLDRWELDHLREHAAALAERLETLEAEAAELRRARDDAED
                     RAGFWHDQTIGYYHDEQERGAVVGLAVDGTLSIVTPPPHR"
     gene            2404..>2732
                     /locus_tag="VNU48_12680"
     CDS             2404..>2732
                     /locus_tag="VNU48_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12180.1"
                     /translation="MGRELKRVAIDFDWPVNKVWQGYVNPHYTAAKCIHCDGTGSSPL
                     ANHLKAQWYGNAPFKPEDRGSVPFLPANAVIRGLAERNIGQAPEFYGSGEAAIVREAQ
                     RLCGHFNR"
BASE COUNT          473 a          964 c          872 g          423 t
ORIGIN      
        1 aaccgatcac gcccgccggg cgaagcatca aggagaaaca cacatgaacc gcgaccccct
       61 cgacatgatc gaaatccccg ctgatgactg cgcgtttgaa gtcgtcgacg acgtgaatcc
      121 gttcttcgcg ccagtcgcga ccgatctgct cgacagcctg ctgggccagt accaagccgc
      181 gcgccgcaac atcgacgcgc ttcacggcgt cgtcatgggc ggcgagttcg gcaacgtcgt
      241 gcactacttc atcgagggca acgccggcga cgatcagctg caccgcagcc tgtacgtcga
      301 caagctcttc gcgatcgacg gcgccgtcgg cgcgctcaat tcggcgttct ggtccaaggc
      361 gctcgcgctg accgatgtct acgcgctgat gccgcaggag cgcaagaacc agtggaacgc
      421 gcagatgcgc agccccatgg gcgtcaagaa gcgaaaccgc agctacggct cgacggcgtc
      481 ggagtgggaa ttggagccgc tgcccgactt cgacgaggcg accgtgcgca cgaccatcgc
      541 cggcctgctg cacgcacgct cgcagttcct ggcggaacgc gtcgacggca tctttcgcaa
      601 cctcagtggc catcacgtca cgaacgcccc ggaggccttc ggcaagcgca tgatcgtcgc
      661 gcgcgtgctc agcgagtacg acacgatcaa ccacgaccgc gccggctatc tccacgatct
      721 gcgctgcgtc atcgccaagt tcatgggccg cgaggagccc gcgcacttcg gcacgacgga
      781 tgcgctcatc aagagcgcca aggagcagcg cggcgaatgg gtctacgccg acggcggcgc
      841 gctcaagctc aaggtgttca aggtcggcac cgcgcacctc gaagtgcacc cggacatggc
      901 gtatcgcctg aatcagattc tcgcgttcat gcacccgcac gcgatccccg cggagttccg
      961 cgcgcggccg gcgcgcaagt ccaaggcctg ggcgctgctg cgccggccgc tgccgtttgc
     1021 tgtcacgtcg ctgattgcca cgatgcgccc gggcgccgag aagattccca acgacttccg
     1081 cgacggcatg cgcacggttc cgaacacgcg gcgcttcgac ttcggcgacc acgacaaagc
     1141 cgcgctggcc gaggccgtgc gcatcatcga gtcgatcggc ggcgtcaaga ccgggcatta
     1201 ctggacgttc gactacgagc ccgccgacgt gctgcgcgag atcgcgacga ctggctgcat
     1261 tcccgatcac gtctcgcatc agttcttccc gaccccggag accgtcgcgg ccgccgtgat
     1321 cgatgccgcg gagatcggcg acggcgacac gtgcttggag ccgagcgccg gccacggcgc
     1381 gctggccgat ctgatgccgc gcgatcggac gcgctgcgtc gaggtctcgc cgcttcactg
     1441 cgcgatactg aaggcgaagg gattcgacac gatcgaagcg gatttcatca agtacgccga
     1501 gggcagcgcg gagcgcttcg atcgcgtcgt cggcaacccg ccgttctgcg atggccgctg
     1561 gcaagcccat acgctggccg ccgcgggtct ggtggcaccc ggcgggcggc tggtgttcgt
     1621 gctgccggca tcggcccgta acgcgctcac cctgccgggc ttcgactgcg cgtggtcgcg
     1681 gatgtacgtg gatgagttcg ccggcaccgg agcggccgtc gtgatcctca ctgctattcg
     1741 gaaggcctga ccccgtggct acgcataccc tacccccggc cggtgattcc ggcccgccca
     1801 tctactaccc gcccgatctg caactgaccc tgaccggcgc cgaggtctgc ccgtgttcgg
     1861 aattgcactc gacacacctg accgattgga gcctttcctt tggcgccatc gacgcacgca
     1921 atgccgtccc gacaggcagt gtgcgcggca tcggcagcgc cgccgtgttc ggcatcgact
     1981 tctgtgtgcg gttctccgag ccgatggcga cgctgtgcga ggtaccggca gcggcgccgg
     2041 cgccatcacc acccgatccg ccgcgggtcg tcgagccgcc gctgtcgcca gccgagcgcc
     2101 gggccctgca gcgccgcctg gaccgctggg aactcgacca cctgcgcgag catgccgccg
     2161 cactggccga gcgcttggag acgctggaag ccgaggccgc cgagctcagg cgagcccgcg
     2221 acgacgccga ggaccgcgcc ggcttctggc acgatcagac catcggctac taccacgatg
     2281 agcaggagcg cggcgccgtg gtcgggctgg ccgtcgacgg cacgctgtcg atcgtcacgc
     2341 caccccctca ccggtgattc ctgatcaccc cgtacctacg taaaccacaa ggagcaatca
     2401 accatgggaa gagaactgaa gcgcgtcgcg atcgatttcg attggcccgt caacaaggtg
     2461 tggcagggct acgtgaatcc gcactacacg gccgcgaagt gcattcattg cgacggcacc
     2521 ggatcgtcgc cgctcgccaa tcacctgaag gctcagtggt acggcaacgc gcccttcaag
     2581 cccgaggatc gcggcagcgt gccgttcctg ccggccaacg ccgtcattcg cggcctagcc
     2641 gagcgcaaca tcggccaagc gccagagttc tacggctccg gtgaggcggc gatcgtgcgc
     2701 gaagcgcagc gcctgtgcgg ccacttcaac cg
//
LOCUS       DATMJB010000598         3034 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_5649, whole genome shotgun sequence.
ACCESSION   DATMJB010000598 DATMJB010000000
VERSION     DATMJB010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5649"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..691)
                     /locus_tag="VNU48_12685"
     CDS             complement(149..691)
                     /locus_tag="VNU48_12685"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HWI12181.1"
                     /translation="MPRCIAFLRAVNVGGRTVKMDALRAAFEALGLAKVQTFIASGNV
                     IFETRARDLAALERRIENQLNERFGFEVHTFIRTEAELTAIVAHRAFGAAAIAAAATH
                     VVGFIADAPDAAAKQLIAGFASAVDRFHVHGRELYWLSTARQSDSKFSNTGFEKLLRR
                     HATFRSIGTLRKLLATLAPR"
     gene            849..1937
                     /locus_tag="VNU48_12690"
     CDS             849..1937
                     /locus_tag="VNU48_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HWI12182.1"
                     /translation="MTTPGPLRRHSPAPARAHLLTLNAGSSSIKYALFARDELGAPVF
                     EGQVEGIGRAGGPATHDAAMARVLVELNARQERVVAVGHRIVHGGARLVQPVRIDDAV
                     LAQMSELIPLAPLHQPHNVAGVRASRNAFAGVPQVACFDTAFHVGQPELNRRFALPQA
                     LFDAGVRRYGFHGLSYESVFAQLLADDPVRAQGRVVIAHLGNGASMCAMHSGRSVATT
                     MSFSPLDGLPMGTRCGRLDAAVVLHLIRQQGRGVDEVERLLFRESGLLGLSGVSSDLR
                     ELEASANPPAAMAIDYFVEHALRELAGMVAALRGIDTLVFTGGIGEHAANVRQRIVDG
                     AAWMGPVDVQVLRTNEEAVIARHTAALV"
     gene            1985..>3034
                     /locus_tag="VNU48_12695"
     CDS             1985..>3034
                     /locus_tag="VNU48_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWI12183.1"
                     /translation="MHDPLAAPKPGAVNEVKTTTCYMCACRCGIRVHLRDGELRYIDG
                     NPNHPLNQGVICAKGSAGIMKQVSPVRLTQPLLRKPGSERGAGEFEPISWDQAFELLT
                     ERLRKIRATDPKKFALFTGRDQMQALTGLFARQFGTPNYAAHGGFCSVNMAAGMIYTV
                     GGSFWEFGGPDLDRAKLFVMIGTAEDHHSNPMKIALAKFKRAGGRFISINPVRTGYSA
                     IADEWIAIKPGTDGALFMALLHELIANDLIDHAFLKRFTNAPQLVVLDDCARQGLFAF
                     DPNPEKGPPGDGRHPHNKLVWDQASGAILNAYPEGIADGCDPALEGHYTLADGTRVAP
                     SFQLLRERVASCTPDW"
BASE COUNT          453 a         1055 c         1059 g          467 t
ORIGIN      
        1 tacgagtacc cgaaggagat cgagttcatc gacgccctgc cgatgacgac caccggcaag
       61 gtgcagcgcc gcgtgctgcg gctgcaggaa gaagagcgcg cccgcaggtc ccgtgcgacg
      121 ctctgacgcg cgacgcctct caggctcttc agcgcggcgc cagcgtcgcg agcagcttgc
      181 gcagcgtgcc gatgctgcgg aaggtggcgt gccgccgcag cagcttctcg aacccggtgt
      241 tcgagaactt cgagtcgctc tgccgagccg tcgacagcca gtacagctcg cgcccgtgga
      301 cgtggaagcg gtcgaccgcg ctggcgaagc ccgcgatcag ctgcttcgcc gccgcatccg
      361 gcgcgtccgc gatgaagccg accacgtgcg tcgccgcggc ggcgatcgcg gcggcaccga
      421 aggcccggtg cgcgacgatc gccgtcaact ccgcctcggt gcggatgaag gtgtggacct
      481 cgaagccgaa gcgctcgttc agctgattct cgatgcgccg ctcgagcgcc gcgaggtcgc
      541 gcgcccgggt ctcgaagatc acgttgccgc tggcgatgaa ggtctgcacc ttcgccaggc
      601 cgagcgcctc gaacgcggcg cgcagcgcgt ccatcttgac ggtgcgcccg ccgacattga
      661 cggcgcgcag gaaggcgatg cagcgtggca tgtgcgccag cgtagcaggg tctgcggtcg
      721 gcccgatggt ctcgatgatg ccgatgccgg ggccgccgct gcctgatctt cgcggcgcca
      781 ctatagtccg ctcagttttt catcttccgg agcgtctttg cgccatggcc cagcctgcct
      841 tcgaggtcat gacgacccct ggccccctgc ggcgtcattc cccggcgccc gcccgagctc
      901 acctgctcac gctgaacgcc ggttcgtcgt cgatcaagta cgcgctgttc gcgcgtgacg
      961 agctcggggc gccggtgttc gaagggcagg tcgagggcat cgggcgggcc ggcggcccgg
     1021 ccacgcacga cgccgcgatg gcacgcgtgc tggtcgagtt gaacgcgcgg caggagcgcg
     1081 tcgtcgcggt cggtcaccgc atcgtgcatg ggggtgcccg gctcgtgcag ccggtgcgga
     1141 tcgacgacgc ggtgctcgcg cagatgagcg agctgatccc gctggcgccg ctgcaccagc
     1201 cgcacaacgt cgccggcgtg cgcgcatcgc gcaacgcgtt cgcgggcgtg ccgcaggtcg
     1261 cgtgcttcga cacggcgttt cacgtcgggc aaccggaact gaaccggcgc ttcgcgctgc
     1321 cgcaggcgct gttcgacgcg ggcgtgcggc gctacggttt tcatgggctg tcgtacgaat
     1381 ccgtcttcgc gcagctgttg gccgacgacc cggtgcgtgc gcagggccgt gtcgtgatcg
     1441 cccacctcgg caacggcgcg tcgatgtgcg cgatgcacag cgggcgctcg gtggccacga
     1501 cgatgagctt ctcgccgctc gacggcctgc cgatgggcac gcgctgcggc cggctcgacg
     1561 cggcggtcgt gctgcacctg atccggcagc agggccgcgg cgtcgacgag gtcgagcgcc
     1621 tgctgttccg cgagtcgggg ctgctcgggc tgtcgggcgt gtcgagcgac ctgcgcgaac
     1681 tcgaagccag cgccaatccc ccggccgcga tggccatcga ctacttcgtc gagcacgcgc
     1741 tgcgcgaact ggccggcatg gtcgccgcgc tgcgcggcat cgacacgctg gtgttcaccg
     1801 gtggcatcgg cgagcacgcc gcgaatgtgc gccagcgtat cgtcgacggt gccgcctgga
     1861 tgggccccgt cgacgtgcag gtactgcgca ccaacgaaga agcggtgatc gcgcggcaca
     1921 cggccgcgct ggtctgacgc tcagctcagg agtccatgtg ttcaagttcc tttcccgcga
     1981 gccgctgcac gatccgctcg ccgcgccgaa gcccggcgcg gtgaacgagg tcaagaccac
     2041 cacctgctac atgtgtgcgt gccgctgcgg catccgcgtg catttgcgcg atggcgagct
     2101 gcgctacatc gacggcaacc cgaaccaccc gttgaaccag ggcgtgatct gcgcgaaggg
     2161 ctcggccggc atcatgaagc aggtgtcgcc ggtgcgcctg acgcagccgc tgctgcgcaa
     2221 gccgggcagc gaacgcggcg cgggcgagtt cgaaccgatc tcgtgggacc aggccttcga
     2281 gctgctgacc gagcggctgc gcaagatccg cgcgaccgac ccgaagaagt tcgcgctgtt
     2341 caccggacgc gaccagatgc aggcgctgac cggcctgttc gcgcgccagt tcggcacgcc
     2401 gaactacgcg gcgcacggtg gcttctgctc ggtcaacatg gccgcgggga tgatctacac
     2461 ggtcggcggc agcttctggg aattcggcgg gcccgatctg gaccgcgcga agctgttcgt
     2521 gatgatcggc accgccgagg accatcacag caacccgatg aagatcgcgc tggccaagtt
     2581 caagcgcgcc ggcgggcgct tcatctcgat caacccggtg cgcaccggct attcggcgat
     2641 cgccgacgag tggattgcca tcaagcccgg caccgacggc gcgctcttca tggcgctgct
     2701 gcacgagctg atcgccaacg acctgatcga ccacgcgttc ctgaagcgct tcacgaacgc
     2761 gccgcaactc gtcgtgctcg acgattgcgc gcgccaggga ttgttcgcgt tcgacccgaa
     2821 ccccgagaag ggcccgccgg gcgacggccg gcatccgcac aacaagctgg tgtgggacca
     2881 ggcgagcggc gcgatcctca acgcctaccc cgagggcatc gccgacggct gcgacccggc
     2941 gctcgaaggc cactacacgc tggctgacgg cacccgcgtc gcaccgtcgt tccagctgct
     3001 gcgcgagcgc gtcgcgagct gcacgccgga ctgg
//
LOCUS       DATMJB010000599         4789 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_854421, whole genome shotgun sequence.
ACCESSION   DATMJB010000599 DATMJB010000000
VERSION     DATMJB010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4789
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854421"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..838)
                     /gene="flgG"
                     /locus_tag="VNU48_12700"
     CDS             complement(56..838)
                     /gene="flgG"
                     /locus_tag="VNU48_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HWI12184.1"
                     /translation="MMRSLWISKTGMEAQQTQLDTISNNLANASTNGYKKSHAVFEDL
                     MYQNLRQAGANSSDQTTLPTGLQLGLGTRAVATSRSFVQGNLQQSSNPLDVAISGQGF
                     FQVQMPDGTTGYTRDGSFQVNANGQLVTNNGMTVQPGITIPANATSVTVGNDGTVSVV
                     LPTQTTPQTVGKLQTANFINPAGLDPLGQNTYAETAASGTPNTGTPGQNGLGQLQQGF
                     VETSNVNVVEELVGMIQTQRAYELNSKAIKTADDMLARLTQL"
     gene            complement(977..2113)
                     /locus_tag="VNU48_12705"
     CDS             complement(977..2113)
                     /locus_tag="VNU48_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI12185.1"
                     /translation="MAVGAVNDREPAAQSVLVVDDVSMNLEIITSQLAKRGYITLVAR
                     SGQEAVERAELARPDLILLDVLMPGINGFETCRRLKQREATRDIPVIFMTALTAIDDK
                     IEGFSVGAVDYVTKPINAEEVATRVVTHLKLDALRRRQAAQNRQLRQEIAAREQDMRS
                     KREEAREEERKHIARELHDELGQHLSAIRLELSFVRMRWGTDNPELREKVQSVQQSID
                     GVIGVVRDLVARLRPVVLDMGIATALKWLVCEFRTSSEVKCDLQIDEQVADLSPWQTS
                     IVFRLVQESLTNIGRHAHAHAAQVSLRRQPHAYVLTVSDDGLGFDPTKIGIKSLGLQG
                     MRERARMLDGRLDLRTRPGEGTSIVVEFPIEPPGRPDHEPGELT"
     gene            complement(2226..3479)
                     /locus_tag="VNU48_12710"
     CDS             complement(2226..3479)
                     /locus_tag="VNU48_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI12186.1"
                     /translation="MPQHTRLGAADSRYAWTRLAVTLALMTIGSGAMYVVAVVLPQVQ
                     AEFGVTRGDASLPYTLMMVGFGLGGVAMGRIADRVGVMWVMLIGAFGLGTGFVAAGLA
                     GSLTGFALAHGLLLGALGSATTFAPLVADTSLWFVRRRGIAVAICASGNYLGGAIWPP
                     IVQHFVATDGWRATYIGLGVFSAVTMALLALPMRPRPPIAMNAPAAHAGGVAPSTRPF
                     GLSMGHAQTLLCIAGVACCVAMAMPQVHIVAYCSDLGFGAARGAEMLSLMLASGVVSR
                     LVSGLICDRIGGLRTLLLGSVLQGLALLLFLPFEGLVSLYVISALFGLFQGGIVPSYA
                     IIVREHFPAAEAGARVGTVIMATLFGMALGGWMSGKVFDLTGSYHAAFLNGIAWNLLN
                     LTIALFLLGRTRTLRRRAGGLAGAG"
     gene            complement(3500..4099)
                     /locus_tag="VNU48_12715"
     CDS             complement(3500..4099)
                     /locus_tag="VNU48_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HWI12187.1"
                     /translation="MTFILAAADDKPAKTDPKVDKLEEQLSFKSRFVLVFGEITDALA
                     HATCRRLLALSEDSEAPITMLISSPGGHVESGDAIFDVIRFVRAPVTTVGSGWVASAG
                     AHIFLAPPKERRLCLPNTRFMIHQPAGGAGGQATDIAIQAKEILRARERLARVVATQT
                     GQTYEKVAVDMERDYWMSADEAIAYGIASRTIERQKDLG"
     gene            complement(4185..>4789)
                     /gene="flgF"
                     /locus_tag="VNU48_12720"
     CDS             complement(4185..>4789)
                     /gene="flgF"
                     /locus_tag="VNU48_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HWI12188.1"
                     /translation="AVPVQGSGASTRVYSLETTTGYDPSAGVVSATGRNLDVAVKGNA
                     WLTVQALDGTEAYTRGGSFEVSNDGTLTTRSGLPVMGDGGPLQVPPNSAVSIGADGTV
                     SAKGSDGKNTAVGKLKLVSPEAPLQRGDDGLFRAADGNPLSADDTARVQDGALEGSNV
                     SAVEAMVAMISAGRQFEAQMKMISTAEADEKAAAQLLGST"
BASE COUNT          710 a         1645 c         1654 g          780 t
ORIGIN      
        1 aggcggtgca tgcggtcgtc gcccatcaca tctgcacggg tctcgaggac gcagctcata
       61 gctgcgtcaa gcgcgccagc atgtcgtcgg cggtcttgat cgccttcgag ttcagctcgt
      121 aggcgcgctg cgtctggatc atgccgacca gctcctcgac gacgttgacg ttcgaggtct
      181 cgacgaagcc ctgctgcagc tggcccaggc cgttctgccc cggcgtgccg gtgttcggcg
      241 tgcctgacgc ggcggtctcg gcgtaggtgt tctggcccag cgggtcgagg ccggccgggt
      301 tgatgaagtt cgcggtctgg agcttgccga ccgtctgcgg cgtggtctgc gtgggcagca
      361 cgacgctgac ggtgccgtcg ttgccgaccg tcacgctggt cgcgttggcc gggatcgtga
      421 tgccgggctg caccgtcatg ccgttgttgg tgacgagctg gccgttcgcg ttgacctgga
      481 acgaaccgtc gcgcgtgtag ccggtggtgc cgtcgggcat ctgcacctgg aagaagccct
      541 ggccgctgat cgcgacgtcg agcgggttgc tgctttgctg caggttgccc tgcacgaagc
      601 tgcgcgaggt cgcgaccgcg cgcgtgccca ggccgagctg cagcccggtg ggcagcgtgg
      661 tctggtcgct gctgttggcg ccggcctggc gcaggttctg gtacatcagg tcctcgaaca
      721 ccgcgtgcga cttcttgtag ccgttggtcg aggcgttggc gaggttgttc gagatggtgt
      781 ccagctgggt ctgctgggcc tccatgccgg tcttcgaaat ccacagggaa cgcatcatgg
      841 cggtgctccg gtaatggcac cgatgatgaa tcgcaggccc gggtcgacaa gcgaggaaaa
      901 gacgggtgga tcgggggctt cttcccgctt gtggcgccga gcttcacatg cgcccgacga
      961 ccgccgaact tcggggctac gtgagttcgc ctggctcgtg atccgggcgc ccgggtggtt
     1021 cgatcggaaa ctcgacaacg atgctcgtgc cctcgcccgg tcgtgtacgc aggtccagcc
     1081 ggccgtcgag catccgggct cgctcgcgca tgccctggag ccccagggac ttgataccga
     1141 tcttcgtcgg atcgaagccc aggccgtcgt cggacacggt caacacatag gcgtgcggtt
     1201 gccgccgcag cgacacctgc gcggcgtgcg cgtgcgcgtg ccgcccgatg ttcgtcaggg
     1261 attcctgcac caggcggaac acgatgctgg tctgccaagg gctgaggtcc gcgacctgtt
     1321 cgtcgatctg caggtcacat ttcacctcgc tcgaagtcct gaattcgcag accagccact
     1381 tcagtgccgt ggcaatgccc atgtccagca ccacgggccg caggcgtgcg accaggtcgc
     1441 gcacgacacc gatgacgccg tcgatggact gctgtacgga ctgcaccttc tcgcgaagtt
     1501 ccgggttgtc cgtgccccag cgcatgcgca cgaacgagag ttccaggcga atcgccgaca
     1561 ggtgctggcc cagctcgtcg tgcaactcgc gggcgatgtg cttgcgttct tcctcgcgtg
     1621 cctcttcccg cttggagcgc atgtcctgtt cgcgcgctgc gatctcctgg cgcagttgcc
     1681 ggttctgcgc ggcctggcgc cggcgcaggg catccagctt cagatgcgtg acgacccgcg
     1741 tcgccacttc ttcggcgttg atcggcttgg tcacgtagtc gactgcaccg acgctgaagc
     1801 cctcgatctt gtcgtcgatc gcagtcaatg ccgtcatgaa gatgaccgga atgtcgcggg
     1861 ttgcctcgcg ctgcttcagc cgcctgcacg tctcgaaccc gttgatgccc ggcatcagga
     1921 catccaggag gatcagatcg ggacgggcga gctctgcgcg ctcgaccgcc tcctgtccgc
     1981 tgcgcgccac cagcgtgatg tagccgcgct ttgcgagttg agaggtgatg atctccagat
     2041 tcatcgacac atcgtcgaca accagaaccg actgcgccgc aggttccctg tcgttcaccg
     2101 cgcccactgc cattgccacg atgtcaccgt cttcggtctg ccgggggaga ggccccagca
     2161 tacgcgcatg caggcgggat cgcgagcccg gttgaagacc gcgagctcgg gtcggctcgc
     2221 ggtcttcaac cggcgccggc caggccgccg gcgcgccggc gcagcgtgcg cgtccggccg
     2281 agcaggaaca gcgcgatcgt caggttcagc aggttccagg cgatgccgtt caggaacgcg
     2341 gcgtggtacg agccggtcag gtcgaacacc ttgcccgaca tccacccgcc cagcgccatg
     2401 ccgaacagcg tggccatgat caccgtgccg acgcgcgcgc cggcctcggc ggccgggaag
     2461 tgctcgcgca cgatgatcgc gtagctcggc acgatgccgc cctggaacag cccgaacagc
     2521 gccgagatca cgtacagcga cacgaggccc tcgaacggca ggaacagcag cagcgccagg
     2581 ccctgcagca ccgagccgag cagcagcgtg cgcagcccgc cgatgcggtc gcagatcagc
     2641 cccgagacga gccggctgac gacacccgag gccagcatca gcgacagcat ctcggcgccg
     2701 cgcgccgcgc cgaagcccag gtcgctgcag tacgcgacga tgtgcacctg cggcatcgcc
     2761 atcgcgacgc agcaggccac gccggcaatg cacagcagcg tctgcgcgtg gcccatgctc
     2821 aggccgaacg gccgcgtcga cggcgcgacg ccgccggcgt gcgcggccgg cgcgttcatc
     2881 gcgatcggtg ggcgcgggcg catcggcagc gcgagcaacg ccatcgtcac cgcgctgaac
     2941 acgcccaggc cgatgtaggt ggcgcgccag ccgtcggtgg cgacgaagtg ctggacgatc
     3001 ggcggccaga tcgcgccgcc caggtagttg ccgctggcgc agatcgccac cgcgatgccg
     3061 cgccggcgca cgaaccagag ggacgtgtcg gccaccagcg gcgcgaaggt cgtcgcgctg
     3121 ccgagcgcac cgagcagcag gccatgcgcg agcgcgaaac cggtcaggct gcccgccagc
     3181 ccggcggcga cgaagccggt gcccagcccg aacgcgccga tcagcatcac ccacatcacg
     3241 ccgacgcgat cggcgattct gcccatcgcg acgccgccca gcccgaagcc gaccatcatc
     3301 agcgtgtagg gcagcgaggc atcgccgcgc gtcacgccga actcggcctg cacctgcggc
     3361 agcaccaccg cgacgacgta catcgcgccg ctgccgatcg tcatcagcgc cagcgtgacg
     3421 gccaggcgcg tccaggcgta gcgcgaatcg gcagcgccta agcgcgtgtg ctgcggcatc
     3481 gtgcgatgcg gcggttgcgt cagcccaggt ctttctggcg ctcgatcgtg cgcgacgcga
     3541 tgccgtacgc gatcgcttcg tcggcgctca tccagtagtc gcgttccatg tcgacggcga
     3601 ccttctcgta ggtctggccg gtctgcgtgg cgacgacgcg cgccagccgc tcgcgggcgc
     3661 gcaggatctc cttggcctgg atcgcgatgt cggtggcctg gccgcccgca ccgccggccg
     3721 gctggtggat catgaagcgc gtgttcggca ggcacaggcg ccgctctttc ggcggcgcga
     3781 ggaagatgtg cgccccggcg ctcgcgaccc agccgctgcc gaccgtggtg acgggcgcgc
     3841 gcacgaagcg gatcacgtcg aagatcgcgt cgcccgattc gacatggccg cccggcgacg
     3901 agatcagcat cgtgatcggc gcctccgagt cctccgacag cgccagcaga cggcggcagg
     3961 tcgcatgcgc aagcgcgtcg gtgatctcgc cgaagacgag cacgaagcgc gacttgaacg
     4021 acagctgctc ttcgagcttg tcgaccttgg ggtcggtctt cgcgggcttg tcgtccgcgg
     4081 cagcgaggat gaaggtcatg ggaactcctg gaaggaatgg aagcgttcca gggagtttac
     4141 ggcgcacccg ccgggcgcgc cggcggcgcc acacggcgcg cgcgtcaggt cgagccgagc
     4201 agctgcgcgg cggccttctc gtcggcctcg gcggtcgaga tcatcttcat ctgcgcttcg
     4261 aactggcgcc cggccgagat catcgcgacc atggcttcga ccgcgctgac gttcgagcct
     4321 tcgagcgcgc cgtcctgcac gcgggcggtg tcgtcggcac tcagcgggtt gccgtcggcg
     4381 gcgcggaaca ggccgtcgtc gccgcgctgc aggggcgcct cgggcgagac cagcttgagc
     4441 ttgccaaccg cggtgttctt gccgtcgctg cccttggcgc tgacggtgcc gtcggcgccg
     4501 atgctgacgg ccgaattcgg cggcacctgc agcgggccgc catcgcccat caccggcagg
     4561 ccgctgcgcg tggtcagcgt gccgtcgttc gacacctcga acgagccgcc gcgtgtgtag
     4621 gcctcggtgc cgtcgagcgc ctgcacggtg agccaggcgt tgcccttgac cgcgacatcg
     4681 aggttgcggc cggtcgcgct gacgacgccg gcactcgggt cgtacccggt cgtggtttcg
     4741 agcgaataga cgcgcgtgct cgcgccgctg ccctgcaccg gcaccgcac
//
LOCUS       DATMJB010000600         3644 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_107510, whole genome shotgun sequence.
ACCESSION   DATMJB010000600 DATMJB010000000
VERSION     DATMJB010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3644
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107510"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VNU48_12725"
     CDS             <1..757
                     /locus_tag="VNU48_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011349352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWI12189.1"
                     /translation="LGTPLYIVHVSCEDSVNAIIRARSHGQRVYGEALAGHLTIDESV
                     YRHENWDIAAAHVMSPPFRAKHHQDALWGALAAGHLHTTATDHCAFCGEQKGMGRSDF
                     SKIPNGCAGVEDRLSVLWDAGVNTGRLTPNEFVRVTSTNAAQIFNLYPRKGAIQPGSD
                     ADLVVWDPAATKTITTAGSLSKVGYNVFEGRQVQGLAKVTLSRGKIVWMDGELRAERG
                     AGRYVKRPAFTPLFDALKRQSALHAPHAVARTA"
     gene            777..2162
                     /locus_tag="VNU48_12730"
     CDS             777..2162
                     /locus_tag="VNU48_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI12190.1"
                     /translation="MPQPDVKAGRLSACQYTTNFGDAHPPLTNAQALIEAERCYYCFD
                     APCINACPTGIDIPSFIRRISEDNLRGAAKAILEANIFGGMCARVCPTEVLCEQACVC
                     NTHEDKPVEIGLLQRHATDHLMASNGAPLFTRAAPTGRRIAVVGAGPAGLACAHRCAM
                     LGHEIVVFESRPKPGGLNEYGLATYKTVDDFAQQEIAWLLTIGGIEIRYGQALGEQVL
                     LADLSAQYDAVFLGLGLGGVNALGLQGEAIAGVRDAVEFIAGLRQTRDYAAVPVGRRV
                     VVIGGGMTAVDAAVQSRKLGAQEVTIVYRRDQPRMPASTYEQDWARRNGVAIREWGVP
                     RALDTNGGQVEAVSFAHVQELNGQLVPTGRHWRIEADTVLKAIGQTLLLADPRLSTLA
                     LRSGRILVDGEGRTSLAQVWAGGDCTYGGRDLTVEAVEHGKIAAHSIDRAFGIQRSGK
                     PFTRTRTGLGA"
     gene            2181..3482
                     /gene="preA"
                     /locus_tag="VNU48_12735"
     CDS             2181..3482
                     /gene="preA"
                     /locus_tag="VNU48_12735"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HWI12191.1"
                     /translation="MADLSTTFAGIRSPNPFWLASAPPTDKEYNVNRAFEAGWGGVVW
                     KTLGEAGPPIVNVNGPRYAALYSQDRRLIGFNNIELITDRPLEVNLDEIARVKRNWPD
                     RAMVVSIMVPCEEHAWQAILPRVEDTGCDGIELNFGCPHGMSERGMGSAVGQVPEYIE
                     MVTRWCKQYTRLPVIVKLTPNVADIKPPALAAQRGGADAVSLINTINSVMGVDLDALL
                     IYPNTGGTGSHGGYCGPAVKPIAQNMVAEIARTPQMAWLPISGIGGITNWRDAAEFIA
                     LGCGNVQVCTAAMTYGFKIVQDMCSGLTEYMDAKGFHNIDAFRGRAVASIVDWNQLNL
                     NHIEKARIAQDLCIQCGRCHVVCEDTSHQAITATKDSQRHFEVIDAECVGCNLCVSVC
                     PVPECITMYALQPGQVDARTGQSVPAAYANWTTHPNNPARV"
BASE COUNT          612 a         1276 c         1237 g          519 t
ORIGIN      
        1 gctgggcacg ccgctgtaca tcgtgcatgt gagttgcgaa gactcggtga acgcgatcat
       61 ccgcgcgcgc agccacggcc agcgcgtcta tggcgaggcc ctggccggcc acctgacgat
      121 cgacgagtcg gtctaccgcc acgagaactg ggacatcgcc gcggcccacg tgatgagccc
      181 gccattccgc gccaagcatc atcaagatgc gctctggggc gcgctggccg ccggccacct
      241 gcacaccacg gcgaccgacc actgcgcgtt ctgcggcgag cagaagggca tgggccgatc
      301 cgacttcagc aagatcccga acggctgcgc cggcgtcgag gaccggctgt cggtgctgtg
      361 ggatgccggc gtcaacaccg ggcggctgac gccgaacgag ttcgtgcgcg tcacctcgac
      421 caacgccgcg cagatcttca acctgtaccc gcgcaagggc gcgattcaac ccggcagcga
      481 cgccgatctc gtggtctggg acccggccgc gaccaagacc atcacgaccg ccggcagcct
      541 cagcaaggtc ggctacaacg tgttcgaggg gcgccaggtg cagggcctgg cgaaggtcac
      601 gctgtcgcgc ggcaagatcg tctggatgga cggcgagctg cgcgccgagc gcggcgccgg
      661 ccgctacgtg aagcgcccgg cgttcacgcc gctgttcgat gcgctgaagc gccagtcggc
      721 gctgcacgcg ccgcacgccg tcgcgcgcac cgcctgattg ctttcgagga ccctcgatgc
      781 cccaacccga cgtcaaggcc ggtcgcctga gcgcgtgcca gtacaccacc aacttcggcg
      841 atgcgcaccc tcccctcacg aacgcgcagg cgctgatcga ggccgagcgc tgctactact
      901 gcttcgacgc accctgcatc aacgcctgcc cgaccggcat cgacatcccg tcgttcatcc
      961 gccgcatcag cgaagacaac ctgcgcggcg cggccaaggc gatcctcgag gccaacatct
     1021 tcggcggcat gtgcgcgcgc gtgtgcccga ccgaggtgct gtgcgagcag gcctgcgtgt
     1081 gcaacaccca tgaggacaag ccggtcgaga tcggcctgct gcaacgccac gcgaccgatc
     1141 acctgatggc atcgaacggc gcgccgctgt tcacgcgcgc ggcgcccacc ggcaggcgca
     1201 tcgcggtcgt cggcgccggc ccggccggcc tggcgtgcgc gcaccgctgc gcgatgctcg
     1261 gccacgagat cgtcgtgttc gagtcgcgac cgaagcccgg cggcctgaac gaatacgggc
     1321 tggccaccta caagaccgtc gacgacttcg cgcagcagga gatcgcctgg ctgctgacga
     1381 tcggcggcat cgagatccgc tacggccagg cgctgggcga gcaggtgctg ctggccgatc
     1441 tgagcgcgca gtacgacgca gtgttcctgg gcctgggcct gggtggcgtc aacgcgctcg
     1501 ggctgcaggg cgaagcgatc gccggcgtgc gcgacgcggt cgagttcatc gccgggctgc
     1561 gccagacgcg cgactacgcg gccgtgccgg tgggccggcg cgtcgtcgtg atcggcggcg
     1621 gcatgaccgc ggtcgacgcc gcggtgcagt cgcgcaagct cggcgcgcag gaggtgacca
     1681 tcgtctaccg ccgcgaccag ccgcgcatgc cggcgtcgac ctacgagcag gactgggccc
     1741 ggcgcaacgg cgtggcgatc cgcgaatggg gcgtgccccg ggcgctggac accaacggcg
     1801 gtcaggtcga ggccgtcagc ttcgcccacg tgcaggagct gaacggccag ctcgtgccga
     1861 ccggcaggca ctggcgcatc gaggccgaca cggtgctgaa agcgatcggc cagacgctgc
     1921 tgctggccga cccgcggctg tcgacgctgg cgctgcgcag tggccgcatc ctggtcgatg
     1981 gcgaaggccg tacatcgctc gcgcaggtct gggccggcgg cgactgcacc tacggcggac
     2041 gcgacctgac ggtcgaggcg gtcgagcacg gcaagatcgc ggcgcactcg atcgatcgtg
     2101 cgttcggcat ccaacgcagc ggcaagccgt tcacgcgcac gcgcactggc ctcggcgcct
     2161 gacctttcga aagcttcacc atggccgacc tcagcaccac cttcgcgggc atccgcagcc
     2221 cgaacccgtt ctggctcgcg tcggcgccgc cgaccgacaa ggaatacaac gtcaatcgtg
     2281 ccttcgaggc cggctggggc ggcgtggtct ggaagacgct gggcgaggcc ggcccgccga
     2341 tcgtcaacgt caacggcccg cgctacgcgg cgctgtattc gcaggaccgg cgcctgatcg
     2401 gcttcaacaa catcgagctg atcacggatc ggccgctgga agtgaacctc gacgagatcg
     2461 cgcgcgtcaa gcgcaactgg ccggaccgcg cgatggtcgt ttccatcatg gtcccttgcg
     2521 aggaacacgc ctggcaggcg atcctgccgc gcgtcgaaga caccggctgc gacggcatcg
     2581 agctgaactt cggctgcccg cacggcatga gcgagcgcgg catgggttcg gcggtgggtc
     2641 aggtgcccga gtacatcgag atggtcacgc gctggtgcaa gcagtacacg cggctgccgg
     2701 tgatcgtgaa gctgacgccg aacgtcgccg acatcaagcc gccggcgctg gcggcgcagc
     2761 gcggcggcgc cgacgcggtg tcgctgatca acacgatcaa ctcggtgatg ggcgtggacc
     2821 tcgacgcgct gctgatctac ccgaacaccg gcggcaccgg ctcgcacggc ggctactgcg
     2881 gcccggcggt caagccgatc gcgcagaaca tggtggccga gatcgcgcgc acgccgcaga
     2941 tggcctggct gccgatctcg ggcatcggcg gcatcacgaa ctggcgcgat gcggccgagt
     3001 tcatcgcgct gggctgcggc aacgtgcagg tctgcaccgc ggcgatgacc tacggcttca
     3061 agatcgtgca ggacatgtgc agcgggctga cggaatacat ggacgccaag ggcttccaca
     3121 acatcgacgc gttccgcggc cgcgccgtgg cgtcgatcgt cgactggaac cagctcaacc
     3181 tgaaccacat cgagaaggcg cgcatagccc aggacctgtg catccagtgc ggccgctgcc
     3241 acgtggtgtg cgaagacacc tcgcaccagg cgatcaccgc gacgaaggac agccaacgtc
     3301 acttcgaagt catcgacgcc gagtgcgtcg gctgcaacct gtgcgtgtcg gtgtgcccgg
     3361 tgcccgagtg catcacgatg tacgcattgc agccgggtca ggtcgacgcg cgtaccggac
     3421 aatcggtgcc ggccgcgtat gccaactgga cgacgcaccc gaacaaccct gcccgtgtct
     3481 gatccgcgtg tctgaacctc tgccctctcc ctcgccctcc accgtcgaag tccaaggcgt
     3541 ctcggtcgtc taccccgcca aggaacacac cgtcaccgcg ctggccgacg tcagcctgaa
     3601 catcagtgaa ggcgagttca tctcgctgat cggcccgtcg ggct
//
LOCUS       DATMJB010000601         3235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_239023, whole genome shotgun sequence.
ACCESSION   DATMJB010000601 DATMJB010000000
VERSION     DATMJB010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239023"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VNU48_12740"
     CDS             <1..636
                     /locus_tag="VNU48_12740"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008811488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWI12192.1"
                     /translation="WYSFTALNNPENHPARSMQDTFYVDVKDANGAWLNLRPHTSPMQ
                     IRYAQAHAKKYAGQAEMPDIRVIAPGRTYRVDSDATHSPMFHQVEGLWIGANVSFKDL
                     KVMYLEFIQAFFETTELKLRFRPSYFPFTEPSAEIDIMFERGPLEGKWLEVSGSGQVH
                     PNVVRHMGLDPERHIGFAFGSGIDRLAMLRYGISDLRAFFEGDLRFLAQFN"
     gene            654..3104
                     /gene="pheT"
                     /locus_tag="VNU48_12745"
     CDS             654..3104
                     /gene="pheT"
                     /locus_tag="VNU48_12745"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWI12193.1"
                     /translation="MQFPESWLREFCNPPITSAELADTLTMAGMEVEDAHPAAPPFRG
                     VVVGEVLAVERHPNADRLSVCRVDVGAAAPLSIVCGAPNVRAGIKVPCALVGAQLPPA
                     DDAKDGAQPFVIKLGQLRGVESQGMLCSARELKLSEDHGGLLILDAGAPVGQDIRAHL
                     KLDDTLLTLKLTPNLAHCLSVYGVARELAALTGAPLRQPVFPPLKASLADKLPVTIEA
                     TDLCGRFSGRIVRNIDTKAPTPAWMVDRLARCGQRSVTALVDISNYVMFEYGRPTHIF
                     DLDKIHDGLVVRWGRVGESLKLLNGTTIEVDEQVGVIADVSDRRESVESLAGIMGGDA
                     TAVSDDTRNIYVEAAFWWPEAVAGRSRRYNFATEAGHRFERGVDPASTVEHLERITQL
                     IVDICGRADTACGVIDDQAPSLPRRAPVSLRVERAAKVIGMPITQAQCEGVMQRLALP
                     FSSVPGVLTVTPPSWRFDLNIEEDLIEEVIKLIGYGRLPDTAPLAALTPQVRSESQRS
                     LDVVRRRMAALDYQETINFSFVDARWEHELAGNADPIKLVNPMAAQLAVMRSGLLGSL
                     IAVLRLNLSRKATRVRVFEAGCVFRRDATVVDGALSVAGVAQTMRLAGLMFGPADALQ
                     WATKDRSVDFFDLKGDIEALLAPRAARFVAAEHPAMHPGRCARIELDGVAIGHAGELH
                     PKWRQAYELPAAPLLFELDAAALTARELPTYTPVQRQQSIWRDLSVIASESVTHEALM
                     QAIAGAAQAGLVKSSRLFDVYKPPAASADIRPGERSLSIRLELLDDEVTLTDARIDAV
                     VAGVLDALSRRLGVRLRA"
BASE COUNT          522 a         1119 c         1110 g          484 t
ORIGIN      
        1 tggtacagct tcaccgcatt gaacaacccc gagaaccacc cggcgcggtc gatgcaggac
       61 accttctacg tcgacgtgaa agacgccaac ggtgcctggc tgaacctgcg tccgcacact
      121 tcgccgatgc agatccgcta cgcccaggcg cacgcgaaga agtacgccgg ccaggccgag
      181 atgcccgaca tccgcgtgat cgcaccgggt cgcacgtatc gcgtcgacag cgacgcgacg
      241 cactcgccga tgttccacca ggtcgagggc ctgtggatcg gtgcgaacgt cagcttcaag
      301 gacctgaagg tgatgtacct ggagttcatc caggccttct tcgagaccac cgaactgaag
      361 ctgcgcttcc gcccgagcta cttccctttc accgagccga gcgccgagat cgacatcatg
      421 ttcgagcgcg gcccgctcga aggcaagtgg ctcgaagtgt cgggctccgg ccaggtgcac
      481 ccgaatgtgg tgcgccacat gggcctcgac cccgagcgtc acatcggctt cgcgttcggt
      541 tccggcatcg accggctggc gatgctgcgc tatggcatca gcgacctgcg cgcgttcttc
      601 gaaggcgatc tgcgcttcct ggcgcagttc aactgactcg tttcgacaag acaatgcaat
      661 ttccagaatc ctggctgcgc gagttctgca acccgccgat caccagcgcc gaactggccg
      721 acacgctgac gatggccggc atggaggtcg aagacgcgca ccccgccgcg ccgccgttcc
      781 gcggcgtcgt ggtcggcgag gtgctggcgg tcgagcgcca tccgaatgcc gaccggctca
      841 gcgtgtgccg cgtcgacgtc ggcgctgccg caccgctgag catcgtctgc ggcgcgccga
      901 acgtgcgcgc cggcatcaag gtgccgtgtg cgctggtggg cgcgcagctg ccacccgccg
      961 atgacgcgaa ggacggcgcc cagccgttcg tgatcaagct cggccagctg cgcggcgtcg
     1021 agagccaggg catgctgtgc tcggcgcgcg agctgaagct ttccgaggac cacggcggct
     1081 tgctgatcct ggacgccggc gcgccggtcg ggcaggacat ccgcgcccac ctgaagctcg
     1141 acgacacctt gctgaccctg aagctcacgc cgaatctggc gcactgcctg agcgtctacg
     1201 gcgtggcacg tgagctcgcg gcgctgaccg gcgcgccgct gcgccagccg gtgtttccgc
     1261 ccctcaaggc gagcctggcc gacaagctgc cggtgacgat cgaagcaacc gatctgtgcg
     1321 gccgcttctc gggtcgcatc gtgcgcaaca tcgacacgaa ggcgccgacg ccggcgtgga
     1381 tggtcgatcg gctggcccgc tgtggccagc gctcggtgac ggcgctggtc gacatctcga
     1441 actacgtgat gttcgagtac ggccggccga cgcacatctt cgatctcgac aagatccacg
     1501 acggtctggt cgtgcgttgg ggccgcgtcg gcgagtcgct gaagctgctg aacggcacga
     1561 cgatcgaggt ggacgagcag gtcggcgtga tcgccgatgt cagtgacagg cgcgagtcgg
     1621 tcgagtcgct cgccggcatc atgggcggcg acgcgacggc ggtctcggac gacacccgga
     1681 acatctacgt cgaggccgcg ttctggtggc ccgaagccgt ggccgggcgt tcgcgccgct
     1741 acaacttcgc gaccgaggcc gggcaccgct tcgagcgcgg cgtcgacccg gcctcgaccg
     1801 tcgagcacct cgagcggatc acgcagctga tcgtcgacat ctgcggccgc gcggacacgg
     1861 cctgcggcgt gatcgacgac caggcgccga gcctgccgcg gcgggcgccg gtcagcctgc
     1921 gcgtcgagcg cgcggccaag gtcatcggca tgccgatcac gcaagcgcag tgcgaaggcg
     1981 tgatgcagcg cctggcactg ccgttcagca gtgtgccggg cgtgctgacg gtcacgccgc
     2041 cgagctggcg cttcgatctg aacatcgagg aggacctgat cgaggaagtg atcaagctca
     2101 tcggctacgg ccggctcccc gacaccgcgc cgctggcggc gctgacaccg caggtgcgca
     2161 gcgaatcgca gcgcagcctg gacgtggtgc gccgacgcat ggcggcgctc gactatcagg
     2221 agacgatcaa cttcagcttc gtcgatgcgc gctgggagca cgagctcgcc ggcaacgccg
     2281 acccgatcaa gctggtcaac ccgatggccg cgcagctcgc ggtgatgcgc tccggcctgc
     2341 tcggcagcct gatcgcggtg ctgcggctca acctgtcccg caaggccacg cgcgttcgcg
     2401 tgttcgaggc gggctgcgtg ttcaggcgcg acgcgacggt ggtcgacggt gcgctcagcg
     2461 tcgccggcgt ggctcagacg atgcggctgg cgggcctgat gttcggccct gcggacgcgc
     2521 tgcaatgggc cacgaaggac cgcagcgtcg acttcttcga cctgaagggc gacatcgaag
     2581 cgctgctggc gccgcgcgcc gcgcgcttcg tcgcggccga acacccggcg atgcacccgg
     2641 ggcgctgcgc gcgcatcgag ctcgacggcg tcgcgatcgg ccatgccggc gaactgcacc
     2701 cgaaatggcg ccaggcctat gagctgccgg ccgcgccact gctgttcgag ctcgacgccg
     2761 cggcgctcac ggcgcgcgag ctgccgacct acacgccggt gcagcgccag cagtcgatct
     2821 ggcgcgacct gtcggtgatc gcgagcgaat cggtcacgca cgaggcgttg atgcaggcca
     2881 tcgcaggcgc cgcgcaggcc ggcctggtca aatcgagccg gctgttcgac gtgtacaagc
     2941 cgcccgcggc cagcgccgac attcgccccg gcgagcgcag cctgagcatc cgtctggagt
     3001 tgctcgacga cgaggtcacg ctgaccgacg cgcgcatcga cgcggtcgtt gccggcgtgc
     3061 tcgacgcgct gagccgacgc ctcggcgttc gcctgcgggc ctgagggaat cgagcatgaa
     3121 catgccagac ccgcacgacg ccgccgacat cgacgaggaa gacgccgtac cgggcgcatc
     3181 gcgcgtcgcc ctgtcgacgc tggagacacc gacactgacc aaggccgaac tggcc
//
LOCUS       DATMJB010000602         2688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_25626, whole genome shotgun sequence.
ACCESSION   DATMJB010000602 DATMJB010000000
VERSION     DATMJB010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25626"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VNU48_12750"
     CDS             <1..1226
                     /locus_tag="VNU48_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWI12194.1"
                     /translation="ALARARVLLPRWREAALALPPHDLLDRIVHEGALRERVAATVPP
                     VQRAAALDAIDAVLAQALTLDGARYATPYGFVRALKRRAIKAASPTHADAVQLLTVHG
                     VKGLEARVVFVMDADPEPQGTETATLLIDWPVEAEQPRCCAFVYSEAACPPSLVALLE
                     VERRARRREELNGLYVAMSRARQRLVFSATEPSRRAPGASWWDRIEPLAAAWTLPEGA
                     SAAPVDAAPAPALLKTLSFVPRPAAQPFAVPERGPDTDATRLGKAVHRVLEWVTGAMA
                     TASDEAHNEFAQAAADEFGAPAGAVGALAQAILHSPDCARFFSGPQLRWAGNEVPVSD
                     AGELLRIDRLVQLDATGGPQWWVLDYKLQHAPQQLADYRAQLLRYRDAVRRVQPGDAV
                     RCAFITGAGAVIEVG"
     gene            1334..>2688
                     /locus_tag="VNU48_12755"
     CDS             1334..>2688
                     /locus_tag="VNU48_12755"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWI12195.1"
                     /translation="MAANALSTAHAHPTRAFGRFELRRLLGKSAGTMVWLALDPRSGA
                     EVMLSLPRVQPVNAAALEHWQREVRLAARLDHPNLARVTEVGVQDHWPFVVVDRAQGI
                     TLPEWLAAHPNPKPAEAVGWVSDLLHGLAFAHDAGVAHLDPQLHSLLIDERGSVRLMA
                     LGSAGDAARAAQDTARAKSLERTMALDPSELRAQRSAAARDLLACGLLLQHLLTGQPP
                     LDQPDTTLVIGRMAPLGRELVRLPWSTPLPIPEALRAIANRCTSGQERLRYQNTRTLL
                     GALTGWLEAQSEDGGGPVGLLLDRLRTVGHLPALPGLAARVARVTSRESQRTDQIADQ
                     VLADTALSFELLRTLNSAQVQGTQVQGNGPVLTLRRIISLIGVNGVHLAANTLRPWPG
                     PLSETQAEALQRTLDRVRLAGHTAQALRPKGYDAQVIYLIAVLQSLGRLMLRYHFADE
                     A"
BASE COUNT          342 a         1017 c          984 g          345 t
ORIGIN      
        1 ccgcgctggc gcgtgcccgc gtcttgctgc cgcgctggcg cgaggccgcg ctggcgctgc
       61 cgccacacga cctgctcgac cgcatcgtcc acgagggcgc gctgcgcgag cgcgtcgccg
      121 cgaccgtgcc gccggtgcag cgcgccgccg cgctggacgc gatcgacgcc gtgctggccc
      181 aggcgctgac gctggacggc gcgcgctacg cgacgcccta cggcttcgtg cgcgccctga
      241 agcggcgcgc gatcaaggcc gcgtcgccga cccacgccga cgcggtgcag ctgctgaccg
      301 tgcacggcgt gaagggcctc gaggcgcggg tcgtgttcgt gatggacgcc gaccccgagc
      361 cgcagggcac cgagaccgcg accctgctga tcgactggcc ggtcgaggcc gagcagccgc
      421 gctgctgtgc cttcgtctat tccgaggcgg cgtgcccgcc gtcgctggtc gcgctgctcg
      481 aggtcgagcg ccgggcgcgc cggcgcgagg agctgaacgg cctgtacgtc gcgatgtcgc
      541 gcgccaggca gcgtctcgtc ttcagcgcga ccgagccgtc gcggcgcgcg cccggcgcct
      601 cgtggtggga ccggatcgag ccgcttgccg cggcgtggac actgccggaa ggtgcgtcgg
      661 cggcgccggt cgatgccgcg ccggcgccgg ccctgctgaa gacgctgagc ttcgtgccgc
      721 ggcccgccgc gcagcccttc gccgtacccg agcgcggccc cgacaccgac gccacgcgcc
      781 tgggcaaggc ggtgcaccgc gtgctcgaat gggtcacggg cgcgatggcg acggcatcgg
      841 acgaggccca taacgagttc gcccaagccg ccgccgacga gttcggcgcg ccggcgggtg
      901 cggtcggcgc gctcgcgcag gcgatcctgc acagcccgga ctgcgcacgt ttcttcagcg
      961 gcccgcaact gcgctgggcc ggcaacgagg tgccggtcag cgacgccggc gagctgctgc
     1021 gcatcgatcg actggtgcag ctcgacgcca ccggcggccc gcagtggtgg gtgctcgact
     1081 acaagctgca gcacgccccg cagcagctgg cggactatcg cgcgcaactg ctgcgctacc
     1141 gcgacgcggt gcgccgcgtg cagccgggcg acgcggtgcg ttgtgcgttc atcaccggtg
     1201 ctggcgccgt gatcgaggtc gggtgaccgc gcgggggccg caatgcggac atttggcggg
     1261 tgatgcgccg cctgatcgac gcaccggaca gccgcccgcc gggtttcaat cgcgcaactt
     1321 catcccactc acgatggccg cgaacgccct gtccaccgcc cacgcccacc cgacccgcgc
     1381 gttcggccgc ttcgagctgc gccgcttgct gggcaagagt gcgggcacga tggtctggct
     1441 cgcgctcgac ccgcgcagcg gcgccgaggt gatgctgagc ctgccgcgcg tgcagcccgt
     1501 caacgccgcg gcgctcgagc actggcagcg cgaggtgcgc ctggccgcgc gcctggacca
     1561 tccgaacctg gcacgcgtca ccgaggtcgg cgtgcaggac cactggccct tcgtcgtggt
     1621 cgaccgcgcg cagggcatca cgctgcccga atggctggcc gcgcacccga atcccaagcc
     1681 cgccgaggcc gtcggctggg tcagcgacct gctgcacggc ctggcgttcg cgcacgacgc
     1741 cggcgtggcc cacctcgacc cgcaattgca cagcctgctg atcgacgagc gcggcagcgt
     1801 gcgcctgatg gcgctcggca gcgccggcga cgccgcgcgc gccgcgcagg acaccgcccg
     1861 cgcgaagtcg ctcgagcgca cgatggcgct ggacccgagc gaactgcgcg cgcagcgcag
     1921 cgccgcggca cgcgacctgc tcgcctgcgg cttgctgctg cagcacctgc tgaccggcca
     1981 gccgccgctc gaccagcccg acactacgct cgtgatcggc cgcatggcgc cgctcggccg
     2041 cgaactggtg cgcctgccgt ggagcacgcc gctgccgatc cccgaggcgc tgcgcgcgat
     2101 cgcgaaccgt tgcacgtcgg ggcaggagcg gctgcgctac cagaacacgc gcaccttgct
     2161 gggcgcgctg accggctggc tcgaggccca gtccgaagac ggcggcggcc cggtcggcct
     2221 gctgctggac cggctgcgca ccgtcggtca cctgccggcg ctgccggggc tggcggcgcg
     2281 cgttgcgcgc gtgaccagcc gcgagagcca gcgcaccgac cagatcgccg accaggtgct
     2341 ggccgacacc gcgctcagct ttgagctgct gcgcacgctg aactcggcgc aggtgcaggg
     2401 tacgcaggtc cagggcaacg ggccggtgct gaccctgcgc cgcatcatct cgctgatcgg
     2461 cgtgaacggc gtgcatctcg cggcgaacac gttgcggccg tggcccgggc cgttgtccga
     2521 gacgcaggcc gaggcgctgc agcgcacgct cgaccgcgtg cggctggccg gacacaccgc
     2581 gcaggcgctg cgccccaagg gctatgacgc gcaggtcatc tacctgatcg cggtgctgca
     2641 gagcctcggg cgcctgatgc tgcgctacca cttcgccgac gaggccga
//
LOCUS       DATMJB010000603         3927 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_490709, whole genome shotgun sequence.
ACCESSION   DATMJB010000603 DATMJB010000000
VERSION     DATMJB010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490709"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /gene="paaC"
                     /locus_tag="VNU48_12760"
     CDS             <1..428
                     /gene="paaC"
                     /locus_tag="VNU48_12760"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HWI12196.1"
                     /translation="WQRLSQSTDAEVAGIAGKAVKEARYHQQHAADWAVRLGDGTDES
                     AARMRSALELLWPYIAEMFEADDIDADAARSGLGPSRAELRDAWRAEVLPVLTEARLT
                     PPAERAFRSTGTQGRHSEHMGYILAEMQHLQRAYPGGAW"
     gene            425..946
                     /gene="paaD"
                     /locus_tag="VNU48_12765"
     CDS             425..946
                     /gene="paaD"
                     /locus_tag="VNU48_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HWI12197.1"
                     /translation="MSTIVGSSSRVDAAWRVLANVPDPEVPAVSVCELGIVRDIVDHD
                     DVLEIVLTPTYSGCPATEVIEHDILHAIDAAGLGPARATLRRAPAWTTDWISADGKRK
                     LLAYGIVPPGPATGAEVPIRFLPRAAKTVALACPRCGSHHTERLAAFGSTACKATWRC
                     LDCKEPFEHFKPI"
     gene            943..2025
                     /gene="paaE"
                     /locus_tag="VNU48_12770"
     CDS             943..2025
                     /gene="paaE"
                     /locus_tag="VNU48_12770"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HWI12198.1"
                     /translation="MSAIFHPLRVREVLPDTAEAVVVAFDVPTELREVFSFTQGQYLT
                     LRTEIAGIDLRRSYSICAGVDDGELRVGVRKVKGGVFSNWINEHLRPGHLISVMAPQG
                     RFFVPIEASARRHHLGIAGGSGVTPILSTMKTVLARERFSDFTLIYGNRALKSTMFKE
                     ELEDLKNKYLTRLTLHHVFSDEHTDAPLNMGVMNRAKIGEFLSTVVPASTIAHAFICG
                     PFQMNDEAEAALLAAGVPEDRIHIERFGLAPQAQQVGAIIHEAKPGDAETAKITIIRD
                     GLKRDISFTKDQPSILDAASAAGLEVPFSCTSGVCGTCRAKLVEGEVRMERNFALDKN
                     EVANGFVLTCQAHPLTPHVVLSFDER"
     gene            2039..>3927
                     /gene="paaZ"
                     /locus_tag="VNU48_12775"
     CDS             2039..>3927
                     /gene="paaZ"
                     /locus_tag="VNU48_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HWI12199.1"
                     /translation="MTTTLQSFIAGRWLGTQGAQTLHSAINGRPVASTHAESIDFGEA
                     VAYARNVGLPALMKMDFQQRASALKALAKYLNEHKEHLYAISAHTGATRADSWIDIEG
                     GSGTLFAYAGVGTNELPSGNLLHEGPAMTLGKKGGFAGTHILVPRGGIAVHINAFNFP
                     IWGLLEKFAPSFLAGMPCLGKPATATSYLTEAAMRLIDQSGILPKGALQLVIGSTGDL
                     LDRLDGRDVVTFTGSADTAAKLRVHPNVVRHAIPFNAEADSLNCAILADDVTPDDDEF
                     DLFVKEVAREMTVKAGQKCTAIRRAIVPRRQLDAVAEKLRARLAKTVVGDPANETVRM
                     GALASHAQQRDVADRVATLMKDAELVYGARDGFDPIGDGVAEGAFFAPTLLLSRTPLT
                     SDAVHDVEAFGPVSTLMPYDGIDEAIALAARGKGSLVGTLVTRSPQIAARVIPVAAAL
                     HGRMLILDREAAAESTGHGSPLPVLKHGGPGRAGGGEELGGIRAVKHYLQRAALQGSP
                     TMLAAVTGEHIRGAKVNESETHPFRRWYEDLSIGDSLLTHRRTVFDADIVAFGGISGD
                     YFYMHFDEIAARESAFGKRIAHGYFVLSAAAGLFVSPAEGPVLANYGLDTLRFVKPVA
                     IGDTIR"
BASE COUNT          649 a         1400 c         1317 g          561 t
ORIGIN      
        1 tgtggcagcg gctctcgcaa tcgaccgatg cggaggtggc cggcatcgcc gggaaggcgg
       61 tcaaggaggc gcgctaccac cagcagcacg cggccgactg ggccgtgcgg ctgggcgacg
      121 gcaccgacga gtcggcagcg cggatgcggt ctgcgctcga gctgctctgg ccgtacatcg
      181 ccgagatgtt cgaggccgac gacatcgatg ccgatgctgc gcgctccggc ctcggcccga
      241 gccgcgccga gctgcgcgat gcgtggcgcg ccgaggtgct gccggtgctg accgaagcgc
      301 ggctgacgcc gccggccgag cgcgcgttcc gcagcaccgg cacgcagggg cggcacagcg
      361 agcacatggg ctacatcctc gccgagatgc agcacctgca gcgcgcctac ccgggcggcg
      421 cgtggtgagc acgatcgtgg gcagcagctc gcgtgtcgac gccgcctggc gcgtgctcgc
      481 gaacgtgccc gaccccgagg tgccggcggt ctcggtgtgc gagctcggca tcgtgcgcga
      541 catcgttgac cacgacgacg tgctcgagat cgtgctgacg ccgacctatt ccggctgccc
      601 cgcgaccgag gtgatcgagc acgacatcct gcatgcgatc gacgccgccg gactcggccc
      661 ggcgcgcgcg accttgcgcc gcgcgccggc ctggacgacc gactggatca gcgcggacgg
      721 caagcgcaag ttgctggcct acggcatcgt cccgcccggc ccggcgaccg gcgccgaggt
      781 accgatccgc ttcctcccgc gtgccgcgaa gaccgtcgcg ctggcctgcc cgcgctgcgg
      841 cagccaccac accgagcgcc tcgccgcctt cggctcgacc gcgtgcaagg caacctggcg
      901 ttgcctggat tgcaaagagc cgttcgaaca cttcaaaccg atatgagcgc catcttccat
      961 ccgctgcgcg tgcgcgaggt cctgcccgac accgccgaag cggtcgtcgt cgcgttcgac
     1021 gtgccgaccg aactgcgcga ggtcttcagc ttcacgcaag gccagtacct gaccttgcgc
     1081 accgagatag ctggcatcga cctgcgccgc tcgtactcga tctgcgccgg cgtcgacgac
     1141 ggcgaattgc gcgtcggcgt gcgcaaggtc aagggcggtg tgttctcgaa ctggatcaac
     1201 gagcaccttc ggcccggcca tttgatcagc gtgatggcac cacaaggccg cttcttcgtg
     1261 ccgatcgaag caagcgcgcg tcgccaccac ctcggcatcg ccggcggcag tggcgtgacg
     1321 ccgatcctgt cgacgatgaa gacggtgctg gcgcgcgaac gctttagcga cttcacgctg
     1381 atctacggca accgcgcgct caagtccacg atgttcaagg aagagctcga ggacctgaag
     1441 aacaagtacc tgacgcgcct gaccctgcac cacgtgttct ccgacgagca caccgacgcg
     1501 ccgctgaaca tgggcgtgat gaaccgcgcc aagatcggcg agttcctgag cacggtcgtg
     1561 ccggcgtcga ccatagccca cgccttcatc tgcggcccgt tccagatgaa cgacgaggcc
     1621 gaggcggcgc tgctcgcagc cggcgtgccg gaggaccgca tccacatcga gcgcttcggg
     1681 ctggcgccgc aagcacagca ggtcggtgcg atcattcacg aagccaagcc gggcgacgcc
     1741 gagacggcca agatcaccat catccgcgac ggcctgaagc gcgacatctc gttcacgaag
     1801 gaccagccca gcatcctcga cgccgcgtcc gctgccggcc tcgaagtgcc gttctcgtgc
     1861 acctcgggcg tgtgcggcac ctgccgcgcg aagctggtcg agggcgaggt gcgcatggag
     1921 cgcaacttcg cgctcgacaa gaacgaggtc gcgaacggct tcgtgctgac ctgccaggcg
     1981 catccgctca cgccacatgt cgtcctttca ttcgacgagc gctgaggaca ccaacaccat
     2041 gaccaccacc ctccagagct tcatcgccgg ccgctggctc ggcacgcagg gcgcgcagac
     2101 cctgcacagc gcgatcaacg gccgcccggt cgcgagcacg cacgccgaga gcatcgactt
     2161 cggcgaagcc gtggcctacg cccgcaatgt cggcctgccg gcgctgatga agatggactt
     2221 ccagcaacgc gcgtccgcgc tgaaggcgct cgcgaagtat ttgaacgaac acaaggaaca
     2281 tctctacgcg atctcggccc acaccggcgc gacgcgcgcc gacagctgga tcgacatcga
     2341 aggcggcagc ggcacgctgt tcgcgtacgc cggcgtcggc accaacgagc tgccctcggg
     2401 caacctgctg cacgaaggcc cggcgatgac cttgggcaag aagggcggct tcgccggcac
     2461 gcacatcctg gtgccgcgcg gcggcatcgc ggtgcacatc aacgcgttca atttcccgat
     2521 ctggggcctg ctcgagaagt tcgcgccgag ctttctcgcc gggatgccgt gcctcggcaa
     2581 gcccgcgacc gcgaccagct acctgaccga agcggcgatg cggctgatcg accaatccgg
     2641 catcctgccc aagggcgcgc tgcagctcgt gatcggctcg accggcgacc tgctcgaccg
     2701 gctcgacgga cgcgatgtcg tcaccttcac cggctcggcc gacacggccg cgaagctgcg
     2761 cgtgcacccg aacgtggtgc gccacgcgat cccgttcaac gccgaggccg actcgctaaa
     2821 ctgcgcgatc ctggccgacg acgtgacgcc ggacgacgac gagttcgacc tcttcgtcaa
     2881 ggaagtggcg cgcgagatga ccgtcaaggc cggccagaag tgcaccgcga tccggcgcgc
     2941 gatcgtgccg cgcaggcagc tcgatgccgt cgccgagaag ctgcgtgcgc ggctcgcaaa
     3001 gaccgtcgtc ggcgacccgg caaacgagac ggtgcgcatg ggcgcgctgg cctcgcacgc
     3061 gcagcagcgc gacgtggccg atcgcgtcgc gacattgatg aaggacgccg agctcgtcta
     3121 cggcgcacgc gacggcttcg atccaatcgg cgatggtgtg gccgagggcg cgttcttcgc
     3181 gccgacgctg ctgctcagcc gcacgccgct gaccagcgac gcggtgcacg acgtcgaagc
     3241 cttcgggccg gtcagcacgc tgatgccgta cgacggcatc gacgaggcga tcgcgctcgc
     3301 ggcgcgtggc aagggcagcc tggtcggcac gttggtgacg cgctcgccgc agatcgccgc
     3361 gcgcgtgatc ccggtcgccg cggcgctgca cggccggatg ctgatcctcg accgcgaggc
     3421 cgcggccgaa tcgaccggcc acggctcgcc gctgccggtg ctcaagcacg gcggcccggg
     3481 ccgcgcgggc ggtggcgagg aactgggcgg catccgcgcc gtcaagcact acctgcaacg
     3541 cgcggcgctg cagggctcgc cgacgatgct cgcggcggtc accggcgaac acatccgcgg
     3601 cgcgaaggtc aacgaatccg agacgcaccc gttccggcgc tggtacgaag acctgtccat
     3661 cggcgactcg ctgctgacgc accgccgcac cgtctttgat gccgacatcg tcgcgttcgg
     3721 cggcatctcg ggcgactact tctacatgca cttcgacgag atcgcggcgc gcgaatcggc
     3781 gttcggcaag cgcatcgccc atggctactt cgtgctgtcg gcggcggccg ggctgttcgt
     3841 ctcgccggcc gaagggcccg tgctcgcgaa ttacggcctc gacacgctgc gcttcgtcaa
     3901 gccggtcgcg atcggcgata cgatccg
//
LOCUS       DATMJB010000604         4414 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_25731, whole genome shotgun sequence.
ACCESSION   DATMJB010000604 DATMJB010000000
VERSION     DATMJB010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25731"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /gene="ureC"
                     /locus_tag="VNU48_12780"
     CDS             complement(<1..334)
                     /gene="ureC"
                     /locus_tag="VNU48_12780"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656514.1"
                     /note="ureases catalyze the hydrolysis of urea into
                     ammonia and carbon dioxide; in Helicobacter pylori the
                     ammonia released plays a key role in bacterial survival by
                     neutralizing acids when colonizing the gastric mucosa; the
                     holoenzyme is composed of 3 ureC (alpha) and 3 ureAB
                     (gamma/beta) subunits; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HWI12200.1"
                     /translation="MATLQRRAYAEMFGPTTGDRVRLADTGLIVEVERDYTLAAGGYG
                     EEVKFGGGKTIRDGMGQSQRLAAEVADTVITNALIIDHWGIVKADIGLKNGLISAIGK
                     AGNPDVQPG"
     gene            complement(337..642)
                     /locus_tag="VNU48_12785"
     CDS             complement(337..642)
                     /locus_tag="VNU48_12785"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HWI12201.1"
                     /translation="MIPGELLIDGPDHELNPGRRSVSLVIENSGDRPIQVGSHYHFAE
                     TNAALRFDRAAARGMRLNIASGTAVRFEPGQQRTVELVDYAGARAVFGFRGLVQGAL"
     gene            complement(656..958)
                     /locus_tag="VNU48_12790"
     CDS             complement(656..958)
                     /locus_tag="VNU48_12790"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HWI12202.1"
                     /translation="MELTPREKDKLLIFTAALLAERRRARGLKLNYPEAVALISAAIM
                     EGARDGKSVAALMSEGKTILSRADVMDGVAELIPEIQMEATFPDGTKLVTVHQPIA"
     gene            1075..>4414
                     /locus_tag="VNU48_12795"
     CDS             1075..>4414
                     /locus_tag="VNU48_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI12203.1"
                     /translation="MRPRLGQGVAAPAGATPVQQVIKVRRDYNSWVARETIEDYALRF
                     TPRSFRKWSALRVANTAFGAASFLVLEAAGGTLLVDYGFVNAFWAILATGLIIFLAGW
                     PISVYAARFGVDMDLLTRGAGFGYIGSTITSLIYASFTFIFFALEAAIMAYAIELAFD
                     IPPVWGYLLCALVVIPLVTHGVTTISRLQVWTQPLWLAMLIVPLAYVLHEHPGVIGEL
                     WRYGGFSGDDEPRAAGFDLMKFGAAMTVGIALITQMGEQADYLRFMPEMEPPDHGVPR
                     STPQGGAPAAPERPRAGRRRRWIGARAKWWAGVIIGGPGWVVPGVLKMLAGALLAFLA
                     IGYSVPPDRAVDPTQMYLVAYNAVFSQYGLAVAATALFVFVSQLKINVTNAYAGSLAW
                     SNFFARLTHSHPGRVVWMVFNTLIALMLMELDVFQALSKVLGLYSNIAISWMMAVVAD
                     LVINKPMGWSPKGIEFKRAHLYDVNPVGVGAMGIASLLSIVAYLGVLGPLAQAFSALI
                     ALVTALVASPLIAWWTKGRYYLARSPSAAAPVDEGSYKRLTRCVICERDYERDDLAHC
                     PAYQGDICSLCCTLDARCNDLCKPHARLSVQWNQLLRGLLPRTVWPYLETGLGHYLLL
                     MAVVVPLLAGLFAMLYTQELPALLEAGGAKGAAVLRLGFVKAFAALLLVSGIVAWWLV
                     LTHKSRQVAQEESNRQTQALNEQTGLLNEQAMTLRREIESHRRTDAELQLAKRQAELA
                     NQAKSRYITTVSHELRTPLNSILGYAQLLEEDASVPPHRHQAVRVIRRGGEHLLSLID
                     RTLDIARIEGGKLRLENKPMRFRDCVQEIVRMFELQAAAKGIEFGCVLTEPLPQLVRA
                     DEKRLRQILINVLGNAVKFTHVGRVEFRVAHAREMAVFEIDDTGPGIAAAEIEHVFEP
                     FARGSEASAAASGGTGLGLTIGKMLTDLMGGEMSVVSTPGTGTLFRIRLFLPEVSAAH
                     PLARAEHAPRVARSGYAGARRRVLVVDNEEVDRDLLASLLEPLGFEIHQAASGHECLA
                     LLPGVAPDAILMDLAMPGIDGWATVRAIRERRLSDAPVAIVSGNAFDKGLENDVGVAA
                     EDFILKPVRIDELLDWLGARLQLVW"
BASE COUNT          611 a         1506 c         1559 g          738 t
ORIGIN      
        1 cgcccggctg cacgtcgggg ttgccggcct tgccgatggc gctgatgagt ccgttcttca
       61 gcccgatgtc ggccttcacg atgccccagt ggtcgatgat cagcgcgttc gtgatcacgg
      121 tgtcggccac ctcggcagcg aggcgctggc tctggcccat gccgtcgcgg atggtcttgc
      181 cgccgccgaa tttcacttcc tcgccatagc cgccggcggc cagcgtgtag tccctctcga
      241 cctcgacgat caggccggtg tcggccaacc gcacgcggtc gccggtggtc gggccgaaca
      301 tctcggcata ggcgcggcgc tgcagcgtgg ccatgctcag agcgctccct gcaccaggcc
      361 gcgaaagccg aagaccgcgc gcgcgccggc gtagtccacg agctcgacgg tgcgctgctg
      421 gccgggctcg aagcgcaccg cggtgcccga cgcgatgttc agccgcatgc cgcgcgccgc
      481 cgcgcggtcg aagcgcagcg ccgcattcgt ttcggcgaag tgatagtgcg agccgacctg
      541 gatggggcgg tcgccgctgt tctcgatcac caggctgacg ctgcgccggc ccgggttcag
      601 ttcgtggtcg gggccatcga tgagcagctc gccgggaatc atcgggcagg gtccttcagg
      661 cgatgggttg gtgcaccgtc accaacttgg tgccgtcggg aaaggtcgct tccatctgga
      721 tctccgggat cagctcggcc acgccgtcca tcacgtcggc gcgcgacagg atcgtcttgc
      781 cttcgctcat cagcgccgcg acgctcttgc cgtcgcgcgc gccttccatg atcgcggcgc
      841 tgatcagcgc caccgcctcg ggatagttga gcttcagacc gcgcgcccga cgccgctcgg
      901 cgagcagcgc ggcggtgaag atcagcagct tgtccttctc gcgcggggtc agttccatcg
      961 gggggatcgg tagcaggagg cgtgccgcga tgctagagga gcgcgggcgc gcttggcatg
     1021 gggcttgcac caactcggtt catgagctcg atgctgcctg ttggtgccga agagatgcgc
     1081 ccgcgcctgg ggcagggcgt ggcggcgcct gccggcgcga cgccggtgca gcaggtcatc
     1141 aaggtccggc gcgactacaa cagctgggtc gcgcgcgaga cgatcgagga ctacgcgctg
     1201 cgcttcacgc cgcgctcgtt ccgcaagtgg tcggcgctgc gcgtggcgaa caccgcgttc
     1261 ggtgccgcgt cgttcctcgt gctcgaggcg gccggcggca cgctgctggt cgactacggc
     1321 ttcgtcaacg cgttctgggc gatcctggcc accgggctga tcatcttcct cgccggctgg
     1381 ccgatcagcg tctacgcggc gcgcttcggc gtcgacatgg acctgctcac gcgcggcgcc
     1441 ggcttcggct acatcggctc gaccatcacc tcgctgatct acgccagctt caccttcatc
     1501 ttcttcgcgc tcgaggcggc gatcatggcc tacgcgatcg agctcgcgtt cgacattccg
     1561 ccggtctggg gttacctgtt gtgcgccctg gtcgtgatcc cgctggtcac gcacggcgtc
     1621 acgacgatca gccggctgca ggtctggacc cagccgctgt ggctggcgat gctgatcgtg
     1681 ccgctggcct acgtgctgca cgaacacccc ggcgtgatcg gcgagctctg gcgctacggc
     1741 ggtttcagcg gcgacgatga gccgcgtgcg gcgggcttcg acctgatgaa gttcggtgcc
     1801 gcgatgaccg tgggcatcgc gctgatcacg cagatgggcg agcaggccga ctacctgcgc
     1861 ttcatgcccg aaatggagcc ccccgaccac ggcgtaccgc ggtcgacccc ccaagggggt
     1921 gcgccggccg ctccggagcg gccgcgcgct ggccggcgcc gccgttggat cggtgcgcgt
     1981 gcgaagtggt gggccggcgt gatcatcggc ggccccggct gggtcgtccc gggggtgctg
     2041 aagatgctcg ccggcgcgct gctcgcgttc ctcgcgatcg gctactccgt gccgccggac
     2101 cgcgcggtcg acccgacgca gatgtacctg gtcgcctaca acgcggtgtt ctcgcagtac
     2161 ggcctcgcgg tcgcggccac ggccctcttc gtgttcgtct cgcagctgaa gatcaacgtc
     2221 acgaacgcgt acgccggctc gctggcgtgg tcgaacttct tcgcgcggct gacccacagc
     2281 cacccgggcc gcgtggtgtg gatggtgttc aacacgctga tcgcgctgat gctgatggag
     2341 ctcgacgtgt tccaggccct gagcaaggtg ctcggcctgt actcgaacat cgcgatctcg
     2401 tggatgatgg ccgtcgtcgc cgatctcgtg atcaacaagc cgatgggctg gtcgcccaag
     2461 ggcatcgagt tcaagcgcgc gcacctgtac gacgtcaatc cggtcggcgt cggcgcgatg
     2521 ggcatcgcgt cgctgctgtc gatcgtcgcc tacctcggcg tgctggggcc gctggcgcag
     2581 gcgttctcgg cgctgatcgc gctggtgact gcgctcgtcg cgtcgccgct gatcgcgtgg
     2641 tggacgaaag ggcgctacta cctggcgcgt tcgccgtcgg ccgccgcgcc ggtcgacgaa
     2701 ggcagctaca agcgcctgac ccgctgcgtg atctgcgagc gcgactacga gcgcgacgac
     2761 ctcgcccatt gcccggccta ccagggcgac atctgctcgc tgtgctgcac cctggatgcg
     2821 cgctgcaacg acctgtgcaa gccgcacgcg cgcctgtcgg tgcagtggaa ccagctgctg
     2881 cgcgggctgc tgccgcgcac ggtctggcct tacctcgaaa ccggcctggg ccactatctg
     2941 ctgctgatgg cggtggtggt gccgctgctc gccgggctgt tcgcgatgct gtacacgcag
     3001 gagctgccgg cgctgctcga agccggcggc gcgaagggcg cggcggtcct gcggctgggc
     3061 tttgtgaagg ccttcgcggc gctgctgctg gtcagcggca tcgtcgcgtg gtggctggta
     3121 ctgacgcaca agagccgcca ggtcgcgcag gaggaatcga accgccagac ccaggcattg
     3181 aacgagcaga cgggcctgct gaacgagcag gcgatgacgc tgcggcgcga gatcgaatcg
     3241 caccgccgca ccgacgcgga actgcagctc gccaagcgcc aggccgagct cgccaaccag
     3301 gccaagagcc gctacatcac gaccgtcagc cacgagttgc gcacgccgct gaacagcatc
     3361 ctgggctacg cgcagctgct cgaggaagac gcctcggtgc cgccgcaccg ccaccaggcc
     3421 gtcagggtga tccgccgcgg cggcgagcac ctgctgtcgc tgatcgaccg cacgctcgac
     3481 atcgcgcgca tcgagggcgg caagctgcgg ctggagaaca agccgatgcg ctttcgcgac
     3541 tgcgtgcagg agatcgtgcg catgttcgaa ctgcaggccg cggccaaggg catcgagttc
     3601 ggctgcgtgc tgaccgaacc gctgccgcag ctggtgcgcg ccgacgagaa gcggctgcgc
     3661 cagatcctca tcaacgtgct cggcaacgcg gtcaagttca cgcatgtcgg gcgggtcgag
     3721 ttccgcgtcg cccacgcgcg cgagatggcg gtgttcgaga tcgacgacac cgggcccggc
     3781 atcgccgcgg ccgagatcga gcatgtgttc gaaccgttcg cgcgcggctc cgaggcgagc
     3841 gcggcggcca gcggtggcac cggcctgggc ctgacgatcg gcaagatgct gaccgacctg
     3901 atgggcggcg agatgagcgt ggtcagcacc ccgggcacgg gcacgctgtt tcgcatccgc
     3961 ctgttcctgc ccgaggtcag cgctgcgcat ccgctggcgc gcgccgagca cgcgccgcgt
     4021 gtcgcccgca gcggctacgc cggcgcgcgc cggcgtgtgc tggtggtcga caacgaggag
     4081 gtcgaccgcg acctgctcgc gagcctgctc gagccgctcg gcttcgagat tcaccaggcc
     4141 gcttcgggcc acgagtgcct cgcgctgctg cccggcgtgg cgcccgatgc gatcctgatg
     4201 gacctcgcga tgcccggcat cgacggctgg gcgacggtgc gcgcgatccg cgaacggcgc
     4261 ctgagcgatg cgcccgtggc gatcgtctcg ggcaacgcgt tcgacaaggg tctggagaac
     4321 gacgtcggtg tcgccgccga ggacttcatc ctgaagccgg tgcgcatcga cgagttgctg
     4381 gactggctgg gcgcgcggct gcaactggtg tggc
//
LOCUS       DATMJB010000605         2911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_203715, whole genome shotgun sequence.
ACCESSION   DATMJB010000605 DATMJB010000000
VERSION     DATMJB010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203715"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..659)
                     /locus_tag="VNU48_12800"
     CDS             complement(24..659)
                     /locus_tag="VNU48_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HWI12204.1"
                     /translation="MFSIIQAAGWPIWPLLLCSIAAFALVLERLSSLRTSRVAPPRLL
                     DEVISVTRAHLPAADVVNKLADNSVLGMVLATGLRAVIAEPRTSEAALRGTFESAGRA
                     AVHRLERYLNTLGTIASAAPLLGLMGTVIGMIEIFGSQAPTGNNPALLAHGISVALYN
                     TAFGLMIAIPSLMFYRYFRGLVDGYTLDMEQAADRLVPHLMRFVVDRRQPN"
     gene            1037..2338
                     /gene="xseA"
                     /locus_tag="VNU48_12805"
     CDS             1037..2338
                     /gene="xseA"
                     /locus_tag="VNU48_12805"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWI12205.1"
                     /translation="MIEAYGTAPARAPWGVAALLREVGELIAGRLGVCTVRGEISGLS
                     RAASGHVYFNLKDADGGAALLRCAMFRRAASLLAFVPAEGQLVEVRGRLGVYEPRGEL
                     QVVVEAMQTAGAGALYEQFLRSKARLEALGLFDPTRKRLLPAYPRAVGVVTSLGAAAL
                     HDVLSALARRAPQVRVVVYASPVQGADAPAALAQAIALASERAEVDVLIVCRGGGSLE
                     DLWAFNDEQVVRAIVASAMPVVCGVGHETYVTLADLAADLRAPTPTAAAELVAPTREA
                     CLGVLDAFARALQRRLRDVLDLQAQRLDRSALRLARPDQMLRQQRERLLSLGHRLAGR
                     LPLALQLQRSQMMPREIALTRSVEANLAAQRQRLDALAGRLGALDPKQVLARGYAWLS
                     DAAGRPVGSMRQLTIGAPLQAVLHDGVADVVVTGVAGQTSR"
     gene            2430..>2911
                     /locus_tag="VNU48_12810"
     CDS             2430..>2911
                     /locus_tag="VNU48_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HWI12206.1"
                     /translation="MEHTLPPLPYALDALAPALSKETLEYHYGKHHNAYVVNLNNLQK
                     GTEFESMPLEEIIKKASGGVYNNAAQIWNHTFFWNCMKPQGGGEPKGALAAAISAKWG
                     SYAAFKEAFTKSAVGNFGSGWTFLVKKADGGVDIVNMGAAGTPLTTADKALLTIDVWE
                     "
BASE COUNT          452 a          966 c         1054 g          439 t
ORIGIN      
        1 aagttcatcg ccatcgcacg agctcagttc ggttggcggc gatcgacgac gaagcgcatc
       61 agatgcggca ccagccggtc ggcggcctgt tccatgtcga gcgtgtagcc gtcgacgagg
      121 ccacggaagt agcggtagaa catcagcgag gggatcgcga tcatcagccc gaacgcggtg
      181 ttgtatagcg ccaccgagat gccgtgcgcg agcagtgccg ggttgttgcc ggtcggcgcc
      241 tgcgagccga agatctcgat catgccgatc accgtgccca tcaggcccag cagcggcgcg
      301 gccgaggcga tcgtgccgag cgtgttcagg tagcgctcga gacggtgcac cgcggcgcgc
      361 ccggccgatt cgaaggtgcc gcgcaacgcg gcctcgctgg tgcgcggctc ggcgatcacc
      421 gcgcgcagcc cggtggccag caccatgccc agcaccgagt tgtcggcgag cttgttgacc
      481 acgtcggcgg ccggcaaatg cgcgcgcgtg accgagatga cttcgtccag caggcgcggc
      541 ggcgcgacgc gcgaggtgcg caggctggac aggcgctcga gcaccagggc gaacgcggcg
      601 atggagcaca ggagcaaggg ccagatcggc cagccggcgg cttgtatgat cgaaaacaat
      661 cgcgcccttt cggtgacgtt tcggcggcgg ccggaggttt cgggacgcgc gattatggcc
      721 ccgttgcgca ggccgccaag cggctcgcgg ccgagcgttg cggcgatgca tccggcgccg
      781 cgaatcgacg cgccgcgcgc tcgcccgaac atccaccgga actgtggata actttgtggg
      841 caactgtgcc tgggcgcgcg ccagggcccg cgctgacaag gtccgcgcta aattgctcaa
      901 actttaggca gaagaaaatc cttgaaaatc aatgacttac acccgtgtga cgcgcctgtg
      961 acagagtgtt ttcgcggcct ggccttgcgt ggcgccgttg tggagttctc ggcccgcgcc
     1021 ggcgctgggt ttgcgggtga ttgaggccta cgggaccgcg cccgcgcgcg cgccctgggg
     1081 cgtggcggcg ctgctgcgcg aagtcggcga actgatcgcc ggtcggctcg gcgtgtgcac
     1141 cgtgcgcggc gagatttcgg gcctgtcgcg agccgccagc gggcatgtct acttcaatct
     1201 gaaggatgcc gacggcggcg cggcgctgct gcgctgcgcg atgttccggc gtgctgcgag
     1261 cctgctcgcg ttcgtgcccg cagagggcca gctcgtcgag gttcgcgggc ggctcggtgt
     1321 gtacgagccg cgcggcgaat tgcaggttgt cgtcgaggcg atgcagaccg ccggtgccgg
     1381 cgccttgtac gagcagttcc tgcgcagcaa ggcgcggctc gaggcgctag gcctgttcga
     1441 cccgacgcgc aagcgcctgc tgccggcgta tccgcgcgcg gtcggcgtcg tcacctcgct
     1501 cggcgccgcg gcgctgcacg acgtgctgag cgcgctggcg cggcgtgcac cgcaggtccg
     1561 ggtcgtcgtc tacgcgagcc cggtgcaggg cgccgacgcg cccgcggccc tggcccaggc
     1621 gatcgcactc gcgtccgaac gcgccgaggt cgacgtgctg atcgtgtgcc gtggcggcgg
     1681 ctcgctggag gatctgtggg ccttcaatga cgagcaggtc gtgcgcgcga tcgtggcgag
     1741 tgcgatgccg gtggtgtgcg gtgtcggcca cgagacctac gtgacgctgg ccgatctggc
     1801 cgccgacctg cgcgcgccca cgccgaccgc cgcggccgag ctcgtcgcgc cgacccgcga
     1861 agcctgcctg ggtgtgctgg acgcgttcgc gcgcgcactg cagcgccgcc tgcgcgatgt
     1921 gctcgacctg caggcgcagc gcctcgatcg cagcgcactg cggctggcgc gaccggatca
     1981 gatgctgcgg cagcagcgcg aacggctgct gtcgctgggc caccggctgg ccggccgcct
     2041 gccgctcgcg ctgcagttgc agcgctcgca gatgatgccg cgcgagatcg cgctgacgcg
     2101 cagtgtcgag gccaacttgg ctgcgcagcg tcagcgcctc gacgcgctcg cggggcgact
     2161 cggcgcgctc gacccgaagc aggtgctcgc gcgcggctac gcgtggctca gcgacgccgc
     2221 cgggcggccg gtcggttcga tgcggcagct caccatcggt gcgccgctgc aggctgtgtt
     2281 gcacgacggc gtggccgacg tggtggtcac cggcgtggcc ggtcagacaa gtcgctgaca
     2341 acctgtcggc cgtcgccgtg cgcggcaaca acgctgcgcc aagctgccta caatggctca
     2401 accccgcaac cacccggaag aggttcacga tggaacacac gctgcccccg ctgccctacg
     2461 cgctcgacgc acttgccccg gcgctgagca aggaaacgct cgagtaccac tacggcaagc
     2521 atcacaacgc ctacgtggtg aacctgaaca atctgcaaaa gggcaccgaa ttcgaatcga
     2581 tgccgctcga agagatcatc aagaaggcca gcggtggcgt ctacaacaac gccgcgcaga
     2641 tctggaacca caccttcttc tggaactgca tgaagccgca gggcggcggc gagccgaagg
     2701 gcgcgctggc tgcggcgatc agcgcgaagt ggggcagcta tgccgccttc aaggaggcgt
     2761 tcacgaagag cgcggtcggc aacttcggtt cgggctggac cttcctggtg aagaaggccg
     2821 acggcggcgt cgacatcgtc aacatgggcg cggccggcac gccgctgacc accgccgaca
     2881 aggcgctgct gacgatcgat gtgtgggaac a
//
LOCUS       DATMJB010000606         4228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_328497, whole genome shotgun sequence.
ACCESSION   DATMJB010000606 DATMJB010000000
VERSION     DATMJB010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328497"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1515
                     /gene="gltA"
                     /locus_tag="VNU48_12815"
     CDS             202..1515
                     /gene="gltA"
                     /locus_tag="VNU48_12815"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWI12207.1"
                     /translation="MTPSDVKATLSFSDGSPTMDLPIYKGTIGPDVIDIRKLYAQTGK
                     FTYDPGFLSTASCNSTITYIDGDKGELLYRGYPIEQLAENCDYLETCYLLLKGELPDT
                     TQKADFTRLVTNHTMVNEQMQFFLRGFRRDAHPMAIMTGLVGALSAFYHDSTDIHNAE
                     HRDIAAIRLIAKMPTLVAMAYKYTIGQPYIYPKNDLSYAGNFMRMMFATPCEEYVPND
                     VLVRAMDRIFILHADHEQNASTSTVRLCGSSGTNPFAVIAAGVACLWGPAHGGANEAA
                     LNMLEDVQQMGGVEKIGDFIRQVKDKNSNVKLMGFGHRVYKNYDPRAKLMRETCHEVL
                     DALGLHNDPIFKLAMALEKIALEDDYFVSRKLYPNVDFYSGIVQRAIGIPVTLFTAIF
                     ALARTVGWIAQLNEMIGDPEYKIGRPRQLFSGAPARDVVALARRA"
     gene            1627..3036
                     /gene="leuC"
                     /locus_tag="VNU48_12820"
     CDS             1627..3036
                     /gene="leuC"
                     /locus_tag="VNU48_12820"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWI12208.1"
                     /translation="MPRTLYDKIWDEHVVHTEDDGTAVLYIDRHLVHEVTSPQAFDGL
                     ELAGRKVWRVSANLAVSDHNVPTTDRSHGITDPISRLQVDTLDANCDRLGITQFKMND
                     RRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLLA
                     RKAKNMLVRVDGKLGAGCSAKDIVLAVIGKIGTAGGTGYTIEFAGAAIRALSMEGRMT
                     VCNMAIEAGARAGLVAVDETTIGYVKGRPYTPTGVEWEQALRYWSGLRSDPGAHFDAV
                     VELDASRIRPQVTWGTSPEMVLAIDDRVPDPDHEKDASKRGAIERALQYMALEPNKLI
                     SDILIDKVFIGSCTNSRIEDLREAAAVVRRLGSRVASNVKLAMVVPGSGLVKEQAERE
                     GLDKIFKNAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSP
                     AMAAAAAIEGHFVDVSRLV"
     gene            3062..3706
                     /gene="leuD"
                     /locus_tag="VNU48_12825"
     CDS             3062..3706
                     /gene="leuD"
                     /locus_tag="VNU48_12825"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWI12209.1"
                     /translation="MTPFRIHTGLVAPMDRENVDTDAIIPKQFLKSIKRSGFGPNLFD
                     EWRYLDHGEPGQDPATRRPNPDFVLNQPRYQGASVLLARKNFGCGSSREHAPWALDQF
                     GFRALIAPSFADIFFNNCFKNGLLPIQLPEVQVARLFDEAAAFIGYALTIDLERQLIV
                     KPDGTTIPFEVQPFRKYCLLGGFDDIGLTLRHADKIRAFEAERLLTKPWLAQRI"
     gene            3775..>4228
                     /locus_tag="VNU48_12830"
     CDS             3775..>4228
                     /locus_tag="VNU48_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWI12210.1"
                     /translation="MKIAILPGDGIGTEIVAQAVRVLGALDLRFETEAALVGGAAFDA
                     HGHPLPEATLKLAQAADAVLFGAVGDWKYDTLERTLRPEQAILGLRKHLGLFANFRPA
                     ICYEQLTHASSLKPELVAGLDILIIRELTGDIYFGQPRGRRASPDGAFE"
BASE COUNT          753 a         1439 c         1382 g          654 t
ORIGIN      
        1 atggacctgt ccgacaacga cctgctcgat ctgctgctgg cgcgcaagca gcccgaaggc
       61 gagttgctgg acgacgatgt ggtgcaggtg ctgcgcatga tgcgcgtgcc cgcgtgacga
      121 gaggcctccg cgtgaatcga gaggccccgc ggccggagac agctggcgta cttctatttt
      181 tggcactatc aaggaaagct catgacaccg tctgacgtga aagccaccct gtcgttctcc
      241 gatggcagcc cgaccatgga cctgccgatc tacaagggca cgatcgggcc cgacgtgatc
      301 gatatccgca agctctacgc ccagaccggc aagttcacct acgatccggg cttcctgtcg
      361 acggcgtcgt gcaattccac gatcacctac atcgacggcg acaagggcga gttgctgtat
      421 cgcggctacc cgatcgagca gctcgccgag aactgcgact acctcgagac ctgttacctg
      481 ttgctcaaag gcgagctgcc tgacacgacg cagaaggccg atttcacccg gctcgtcacg
      541 aaccacacga tggtcaacga gcagatgcag ttcttcctgc gcggcttccg ccgcgacgcg
      601 cacccgatgg cgatcatgac cgggctggtc ggggcgctgt cggccttcta tcacgacagc
      661 accgacatcc acaacgccga gcaccgcgac atcgccgcga tccgcctgat cgccaagatg
      721 cccacgctgg tggcgatggc ctacaagtac accatcggcc agccgtacat ctacccgaag
      781 aatgatcttt cgtacgccgg caacttcatg cgcatgatgt tcgccacgcc ctgcgaggag
      841 tacgtgccga acgacgtgct ggtgcgcgcg atggaccgca tcttcatcct gcacgccgac
      901 cacgagcaga acgcctcgac gtccaccgtg cggctgtgcg gctcgtcggg cacgaacccg
      961 ttcgcggtga tcgccgccgg tgtcgcctgc ctgtggggcc cggcgcacgg cggcgccaac
     1021 gaggcggcgc tgaacatgct ggaagacgtg cagcagatgg gcggcgtcga gaagatcggc
     1081 gacttcatcc ggcaggtgaa ggacaagaac tcgaacgtca agctgatggg cttcggccac
     1141 cgcgtctaca agaactacga cccgcgcgcc aagctgatgc gcgagacctg ccacgaagtg
     1201 ctggacgcgc tcggcctgca caacgacccg atcttcaagc tcgcgatggc gctcgagaag
     1261 atcgcgctcg aagacgacta cttcgtgtcg cgcaagctct acccgaacgt cgacttctat
     1321 tccggcatcg tgcagcgcgc gatcggcatt ccggtgacgc tgttcacggc gatcttcgcg
     1381 ctcgcgcgca cggtgggctg gatcgcgcag ctgaacgaaa tgatcggcga ccccgagtac
     1441 aagatcggcc gcccgcgcca attgttcagc ggcgcgccgg cgcgcgacgt ggtggcgctg
     1501 gcccggcgcg cctgagtgcc ggtgagtgct ggtgagcgcg ggagcatcgg caaacgcgcg
     1561 atccgggctg cggcgggggc aaggccctaa aatcacagct tcgctgtacc tccgccgcac
     1621 gccaccatgc cccgcacgct gtacgacaag atctgggacg aacacgtcgt ccacaccgaa
     1681 gacgacggca ccgccgttct gtacatcgac cgccatctgg tccatgaagt gaccagcccg
     1741 caggccttcg acgggctcga gctggccggc cgcaaggtct ggcgcgtctc ggcgaatctg
     1801 gcggtgagcg accacaacgt gccgaccacc gaccgctcgc acggcatcac cgacccgatc
     1861 tcgcggctgc aggtcgacac gctcgatgcg aactgcgatc ggctgggcat cacgcagttc
     1921 aagatgaacg accgccgcca gggcatcgtc catgtgatcg gcccggaaca gggtgccacg
     1981 ctgccgggca tgacggtcgt gtgcggcgac tcgcacacct cgacgcatgg cgccttcggc
     2041 gcgctcgcgc acggcatcgg caccagcgag gtcgagcacg tgctggcgac gcagacgctg
     2101 ctggcgcgca aggcgaagaa catgctcgtg cgcgtcgacg gcaagctcgg tgcgggctgc
     2161 agcgcaaagg acatcgtgct cgcggtgatc ggcaagatcg gcaccgcggg tggcaccggc
     2221 tacacgatcg agttcgccgg cgcggcgatc cgcgcgctca gcatggaagg gcgcatgacg
     2281 gtgtgcaaca tggcgatcga ggccggtgcg cgcgccggtc tggtggccgt cgacgagacc
     2341 acgatcggct acgtcaaggg ccggccatac acgccgacgg gcgtcgaatg ggaacaggcg
     2401 ctgcgctact ggagcggctt gcgttccgac cccggcgcgc atttcgatgc cgtcgtcgaa
     2461 ctcgacgcgt cgcgcatccg cccgcaggtc acctggggca cctcgcccga gatggtgctt
     2521 gcgatcgacg accgtgtgcc cgaccccgac cacgagaagg acgcaagcaa gcgcggcgcg
     2581 atcgagcgcg cgctgcaata catggcgctg gagccgaaca agctgatctc ggacatcctg
     2641 atcgacaagg tcttcatcgg ctcgtgcacc aactcgcgca tcgaggacct gcgcgaggcg
     2701 gcggcggtgg tgcgtcggtt gggcagcagg gtcgcgtcca acgtcaagct cgcgatggtc
     2761 gtgccgggtt ccggcctcgt gaaggagcag gccgagcgcg aggggctcga caagatcttc
     2821 aagaacgccg ggttcgagtg gcgcgagccc ggctgctcga tgtgcctggc gatgaacgcc
     2881 gaccggctcg agcccggcga gcgctgcgca tcgaccagca accgcaactt cgaaggccgc
     2941 cagggcgccg gcggccgcac ccacctggtg agcccggcga tggcggcggc cgcagccatc
     3001 gaaggccatt tcgtcgacgt cagccgcctc gtgtgagcgc gccgcattcc agggtgaacc
     3061 catgactccg tttcgcatcc acaccggcct ggtcgcgccg atggaccggg aaaacgtcga
     3121 caccgacgcg atcatcccga agcagttcct gaagtcgatc aagcgctcgg gcttcggccc
     3181 gaacctgttc gacgaatggc gctacctcga ccacggcgag ccggggcagg acccggcgac
     3241 gcgccggccg aaccccgact tcgtgctgaa ccagccgcgc taccagggcg cgtcggtgct
     3301 gcttgcgcgc aagaacttcg gctgcggctc gtcgcgcgag cacgcgccgt gggcgctcga
     3361 tcagttcggc ttccgcgcgc tgatcgcgcc cagcttcgcc gacatcttct tcaacaactg
     3421 cttcaagaac ggcttgctgc cgatccagct gccggaggtg caggtggcgc gtctgttcga
     3481 cgaggccgcg gccttcatcg gctatgcgct gacgatcgat ctcgagcggc agctgatcgt
     3541 caagcccgac ggcacgacga tcccgttcga ggttcagccg tttcgcaagt actgcctgct
     3601 cggcggcttc gacgacatcg gcctgaccct gcgccacgcc gacaagatcc gtgcgttcga
     3661 ggccgagcgg ctgctgacca agccgtggct ggcgcagcgc atctgagccg acgcatcaaa
     3721 ctctgcgaga aggtgctcgc cgagcgcccc ggtcatcacc aaaggaactg actcttgaag
     3781 atcgccatcc tgccgggcga cggcatcggc accgagatcg ttgcgcaggc cgtgcgcgtg
     3841 ctcggtgcgc tcgacctgcg cttcgagacc gaggccgccc tggtcggcgg tgcggcgttc
     3901 gacgcccacg gccacccgct gcccgaggcg acgctgaagc tcgcgcaggc cgccgatgcg
     3961 gtgctgttcg gcgcggtcgg tgactggaag tacgacacgc tggaacgtac cctgcgcccc
     4021 gagcaggcga tcctcgggct gcgcaagcat ctgggcctgt tcgcgaactt ccgcccggcg
     4081 atctgctatg agcagctgac gcacgcgtcg agcctgaagc cggagctggt cgcgggcctg
     4141 gacatcctga tcattcgcga gctcaccggc gacatctact tcggccagcc gcgcggacgc
     4201 cgcgccagcc ccgacggcgc cttcgaag
//
LOCUS       DATMJB010000607         6910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_144761, whole genome shotgun sequence.
ACCESSION   DATMJB010000607 DATMJB010000000
VERSION     DATMJB010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144761"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VNU48_12835"
     CDS             complement(<1..547)
                     /locus_tag="VNU48_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI12211.1"
                     /translation="MDSFALLLAATLNSGTVLAFAALGLLINERAGIVNLGAEGMMLV
                     AAVAGYATAVHTGSDTLAFAGGAAAGALLAAAFGVLVIWLNTNQYATGLALSLFGVGF
                     SAFVGIGYTQAKLGERPNFAVPYLSDIPFVGPAFFRQHPMVYLAIALTAALAWFLYRS
                     RAGLVLRAVGESPESAHALGYP"
     gene            complement(549..1670)
                     /locus_tag="VNU48_12840"
     CDS             complement(549..1670)
                     /locus_tag="VNU48_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI12212.1"
                     /translation="MLKLEARPEQSKLMSVMSPVLALAITVVIGTILFMLLGKDPVRG
                     LYVFFVEPLKSVYALTELAMKATPLILIALGLAVCFRSNVWNIGAEGQFVVGAIAAAG
                     VAMLADKDTGRWIVAAVLVAGVLGGMVWGGIVAFLRDRFNANEILVSLMLVYVADQVL
                     GYLVYGPWKDPAGFNFPQTITFLKSTAVPKLFTGFRVNIGLILALLAVGGFWVLLFRT
                     YAGFQLQVGGLAPAAARYAGFSSRKALWTALLLSGGMAGLAGALEVAGPLGQLTPYVP
                     AGYGFAAIIVAYVGRLHPVGIIFSAILMSMFYIGGELAQSRLGLPKSLTGVFQGLLLF
                     TLLACDTLIVYRIRWQAARPVPPKAMPIPAATLTTGKGA"
     gene            complement(1657..3189)
                     /locus_tag="VNU48_12845"
     CDS             complement(1657..3189)
                     /locus_tag="VNU48_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI12213.1"
                     /translation="MLRLELVNISKQYPAVKANDAVNLKVEPGEIHAVLGENGAGKST
                     MMKMIYGAVRPDEGEIRWNGQAVDIRNPQEARHLGISMVFQHFSLFDTLTAAENVWLG
                     LDKSLSLAEVTQRMMKVEKEYGLDVEPLRPVHTLSVGERQRVEIVRALMTNPKCLILD
                     EPTSVLTPQAVDKLFVTLRKLAAEGTSILYISHKLDEIRALCTNCTVLRGGKVTGTVD
                     PRTETNAGLSRLMIGAEPPQLQHLQARLGAVVLDVRGLSLPKEDHFGMNLTDIRLQVK
                     AGEIVGIAGVSGNGQQELMAAMSGEDTRSPPDSIHLFGKDISNHSPRKRRREGLHFVP
                     EERLGRGAVPTLSLAANTLLTRTEAVDRSGWVRTAEVTKLAQELIRRFNVKAGGPDAA
                     AKSLSGGNLQKFIVGREIHANPKLLIVSQPTWGVDVGAAAQIRGELLALRDQGCALLV
                     VSEELDELFEICDRLVVIAQGRVSPSVATSAATTLMIGEWMSGLWDKRARQNEESLHA
                     QA"
     gene            complement(3217..4557)
                     /gene="guaD"
                     /locus_tag="VNU48_12850"
     CDS             complement(3217..4557)
                     /gene="guaD"
                     /locus_tag="VNU48_12850"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HWI12214.1"
                     /translation="MNSTIVSSERIALRGDLLDFVAEPGWGEVDSAAVRFRPDHWLLV
                     ENGRIVGAQLQEPDTGWVRHDHSGRLILPGFIDTHVHMPQLDVIASYGSELLAWLETY
                     TFPAELRFADPAHSHAGAALFVDALLAHGTTSAVVFATTHKVSADALFGAALQRGMRI
                     ITGKVLQDRHSPDGLRDDVVQAERDCIDLIERWHGVARLAYAVTVRFAPTSSPEQLAM
                     AGALCAADASLYMQTHVAENRAEVAWVRELFPQARSYLDVYARAGLLHPRSVLAHGIW
                     LDDADRSALQRAGAQIAHSPSSNLFLGSGLFDWPAARAAGAAVSLASDVGGGTSLSLL
                     ETMGDAYKVQAMAGQRLSAWTALHAATRGAAQALHLADEIGSLEAGTVADVCVWDWAA
                     DPVAARRMAVARSLHERAFAWMTMADDRNLVAAFVAGVPRHRRGEGAVRPQELA"
     gene            complement(4582..5592)
                     /locus_tag="VNU48_12855"
     CDS             complement(4582..5592)
                     /locus_tag="VNU48_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWI12215.1"
                     /translation="MTQRLESDKIELYLIRVLKTLITERSVSRAAMRLQSTQPAVSAQ
                     LKRLRALTGDPLLVRAGNNMAPTDTALRLLDPATRLLQDADRLFSPRAHQRGFDPAAS
                     AGTFRIAASDYLDPLFLPELVAHLKHVSPGMRLELMPLSGEFDYRRSLAAAEVDLVIG
                     NWLKPPEELHLGRLMSDEIVCLVAEEHPVVRAAATRAWTPERYLACEHVAPMPFYAGA
                     HGASPGVIDEHLASLGLNRDVMVRSAHFGLIPLMVAQSLLVLTTGRQFCARYVDSLPV
                     RIVRCPVPFPPLTYYQLWHDLTHASASGRWLREQVRDVARNLAGHGMLRRTTPARADA
                     AA"
     gene            5689..>6910
                     /gene="xdhA"
                     /locus_tag="VNU48_12860"
     CDS             5689..>6910
                     /gene="xdhA"
                     /locus_tag="VNU48_12860"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HWI12216.1"
                     /translation="MSDPVPSRPIRFFHRGAVVEVSDAAATRSVLDWLREDARCTGTK
                     EGCNEGDCGACTVVIGEAAAPGDPAAVRGLSLKTVNACIQFLPTLDGKALFTVEDLKA
                     MNADALHPVQQAMVECHGSQCGFCTPGFVMSLWSAYEHGRAAGSQPTRQQLADELSGN
                     LCRCTGYRPILDAGQRMFDLPPVALDTAPVLAALRALGSDTSLRYVAAGSTERFFAPT
                     TLDELAALREANPQAQLVAGSTDVGLWVNKQFRALGDILYVGAVDELKRIEERDGELR
                     IGAGASLEAAFAALAKRFPDLTDVWLRFASPPIRNAGTMGGNVANGSPIGDSPPVLMA
                     LDAQIELRRGARVRRLPLPEFYVDYMKNHLEPGEFVQAIHVPLNGPARQVRGYKISKR
                     YDSDISAVCGAFAIE"
BASE COUNT         1053 a         2397 c         2460 g         1000 t
ORIGIN      
        1 ccgggtagcc cagcgcatgc gccgattcgg gcgactcgcc caccgcgcgc agcaccagcc
       61 cggcgcgcga gcggtacagg aaccaggcca gcgccgcggt cagcgcgatc gcgaggtaga
      121 ccatcgggtg ctgcctgaag aaggccgggc cgacgaacgg gatgtccgac aggtagggaa
      181 cggcgaagtt cggccgttcg ccaagcttcg cctgcgtgta gccgatgccg acgaaggccg
      241 agaagccgac gccgaacagg ctcagcgcca ggccggtcgc gtactggttg gtgttgagcc
      301 agatcaccag cacgccgaac gcggcggcga gcagcgcgcc ggccgcggcg ccgccggcga
      361 acgcgagcgt gtcgctgccg gtgtgcaccg cggtcgcgta gccagccacg gcggcgacca
      421 gcatcatgcc ctcggcgccg aggttgacga tgccggcgcg ctcgttgatc agcaggccca
      481 aggcggcgaa cgcgagcacg gtgccggagt tcagcgtcgc ggccagcagg agtgcaaagg
      541 agtccatgtc aggcgccctt tccggtggtg agcgtggcgg ccgggatcgg catcgccttc
      601 ggcggtacgg gccgcgccgc ctgccagcgg atgcggtaga cgatcaaggt gtcgcaggcc
      661 agcagcgtga acagcagcag gccctggaac acgccggtca gcgacttcgg caggcccagg
      721 cgcgactgcg cgagctcgcc gccgatgtag aacatgctca tcaggatcgc cgagaagatg
      781 atgcccaccg ggtgcagccg cccgacgtag gcgacgatga tcgcggcgaa gccgtagccg
      841 gccggcacgt acggcgtgag ctggccgagc ggcccggcga cttcgagcgc acccgccagg
      901 cccgccatgc cgcccgacag cagcagcgcg gtccacagcg ccttgcgcga cgagaagccg
      961 gcgtagcgcg ccgccgccgg cgccaggccg ccgacctgca actggaagcc ggcgtaggtg
     1021 cggaacagca gcacccagaa cccgccgacc gcgagcagcg ccaggatcag cccgatgttg
     1081 acgcgaaagc cggtgaacag cttcggcacc gcggtcgact tcaggaacgt gatggtctgc
     1141 ggaaagttga acccggcggg gtccttccac ggcccgtaga ccaggtagcc gagcacctgg
     1201 tcggccacgt agaccagcat caggctgacc aggatctcgt tggcgttgaa gcggtcgcgc
     1261 aggaacgcga cgatgccgcc ccagaccatg ccgccgagca cgccggcgac cagcaccgcg
     1321 gcgacgatcc agcgcccggt gtccttgtcg gccagcatcg ccacgccggc tgcggcgatc
     1381 gcgccgacga cgaactggcc ttcggcgccg atgttccaca cgttcgagcg gaagcacacc
     1441 gccaggccga gcgcgatcag gatcagcggc gtggccttca tcgccagttc ggtcagcgcg
     1501 tagacgctct tcagcggctc gacgaagaac acgtacaggc cgcgcaccgg gtccttgccg
     1561 agcaacatga acaggatcgt gccgatcacg accgtgatcg ccagcgcgag caccggcgac
     1621 atcaccgaca tcagcttcga ctgctcgggg cgggcttcaa gcttgagcat ggaggctctc
     1681 ctcattctgg cgggcgcgct tgtcccagag tccggacatc cactcgccga tcatcagggt
     1741 cgtcgcggcc gaggtggcca cgctcggcga gacgcggccc tgcgcgatca cgaccagccg
     1801 gtcgcagatc tcgaacagct cgtcgagctc ttcgctgacg accagcagcg cacagccctg
     1861 gtcgcgcagc gccagcagtt cgccgcggat ctgcgcggcg gcgccgacgt ccacacccca
     1921 ggtcggctgc gagacgatca gcagcttcgg gttggcgtgg atctcgcgcc cgacgatgaa
     1981 cttctgcagg ttgccgcccg acaggctctt ggccgccgcg tccgggcccc cggccttgac
     2041 gttgaagcgg cggatcagtt cctgcgcgag cttcgtgacc tcggccgtgc gcacccagcc
     2101 ggagcggtcg acggcctcgg tgcgcgtcag caaggtgttc gcggcgagcg acaaggtcgg
     2161 caccgcgccg cggcccaggc gttcctcggg cacgaagtgc aggccctccc tgcggcgttt
     2221 tcgcggcgag tgattcgaga tgtccttgcc gaacaaatgg atcgaatccg gcggcgagcg
     2281 cgtgtcctcg cccgacatcg cggccatcag ttcctgctgg ccgttgcccg agacgccggc
     2341 gatgccgacg atctcgcccg ccttcacctg cagccggatg tcggtcaggt tcatgccgaa
     2401 gtggtcttcc ttcggcaggc tcaagccgcg cacgtcgagc acgaccgcgc cgagcctggc
     2461 ctgcaggtgc tgcagctgcg gcggctcggc gccgatcatc aggcggctca gtccggcgtt
     2521 ggtctcggtg cgcgggtcga ccgtgccggt gaccttgccg ccgcgcagca ccgtgcagtt
     2581 ggtgcacagc gcccggatct cgtcgagctt gtggctgatg tacaggatgc tggtgccttc
     2641 ggcggcgagc ttgcgcaagg tgacgaacag cttgtcgacc gcctgcggtg tcagcaccga
     2701 ggtcggctcg tccaggatga ggcacttcgg attggtcatc agcgcgcgca cgatctcgac
     2761 gcgctggcgc tcgccgaccg acagcgtgtg caccggccga agcggctcga cgtccagccc
     2821 gtattccttc tcgaccttca tcatgcgctg cgtgacctcg gccaggctca gcgacttgtc
     2881 gaggccgagc cagacgttct cggccgcggt gagcgtgtcg aacaggctga agtgctggaa
     2941 caccatgctg atgcccaggt gccgtgcctc ctgcgggttg cggatgtcca ccgcctggcc
     3001 gttccagcgg atctcgccct cgtcggggcg gaccgcgccg tagatcatct tcatcatcgt
     3061 cgacttgccg gcgccgttct cgccgagcac ggcgtggatc tcgccgggct cgaccttcag
     3121 gttcaccgcg tcgttcgcct tcacggcggg gtactgcttg ctgatgttga cgagttcgag
     3181 tcggagcatg gagatttcct tcgagtctgg tgtcgttcag gcaagttctt gcgggcgcac
     3241 ggcgccttcg ccgcgccgat ggcgcggcac gccggcgacg aacgcggcaa cgaggttgcg
     3301 atcgtcggcc atcgtcatcc acgcgaaggc gcgctcgtgc aggctgcgtg ccacggccat
     3361 gcggcgcgcc gcgaccgggt cggcggccca gtcccagacg cacacgtcgg ccacggtgcc
     3421 ggcctcgagc gagccgatct cgtcggccag gtgcaacgcc tgcgccgcgc cgcgcgtggc
     3481 tgcgtgcagc gccgtccacg cgctcaggcg ctggcccgcc atggcctgca ccttgtaggc
     3541 gtcacccatg gtctcgagca gcgacaggct ggtgccgccg ccgacatcgc tggccaggct
     3601 gaccgcggca ccggccgcgc gcgcggcggg ccagtcgaac agcccgctgc ccaggaacag
     3661 gttcgacgac gggctgtgcg cgatctgtgc gccggcgcgc tgcagcgcgc tgcggtcggc
     3721 gtcgtcgagc cagatgccgt gcgccagcac gctgcgcggg tgcagcaggc cggcgcgcgc
     3781 gtagacgtct aggtagctgc gcgcctgcgg aaacagttcg cgcacccagg ccacttcggc
     3841 gcggttctcg gcgacgtggg tctgcatgta cagactcgcg tcggccgcgc acagtgcgcc
     3901 ggccatcgcg agctgctcgg gcgagctggt cggcgcgaag cgcaccgtca ccgcgtaggc
     3961 gagccgggcg acgccgtgcc agcgctcgat cagatcgatg cagtcgcgct cggcctggac
     4021 cacgtcgtcg cgcaggccgt cgggcgagtg gcggtcctgc agcaccttgc cggtgatgat
     4081 gcgcatgccg cgttgcagcg ccgcgccgaa cagcgcgtcg gccgagacct tgtgcgtggt
     4141 cgcgaacacc accgccgagg tcgtgccgtg cgcgagcagc gcatcgacga acagggccgc
     4201 gccggcgtgc gaatgcgccg ggtcggcgaa gcgcagctcg gccgggaagg tgtaggtctc
     4261 gagccaggcc agcagctcgc tgccgtagct ggcgatcacg tcgagctgcg gcatgtggac
     4321 gtgggtgtcg atgaagccgg gcaggatcag gcggccgctg tggtcgtggc gcacccagcc
     4381 ggtatcgggt tcttgcagct gggcgcccac gatgcggccg ttctcgacca gcagccagtg
     4441 atccgggcgg aagcgcaccg cggccgagtc gacctcgccc cagccgggct cggcgacgaa
     4501 gtcgagcaga tcgccgcgca gcgcgatgcg ctcggaggac acgatggtgg agttcatggg
     4561 tgcaggcgcg aaggtcgggg ctcaggccgc ggcgtcggcg cgcgccggcg tcgtgcgccg
     4621 cagcatgccg tgcccggcga ggttgcgtgc cacgtcgcgc acctgctcgc gcagccaccg
     4681 gccggaggcc gaggcgtgcg tcaggtcgtg ccagagctgg tagtaggtca gcggcgggaa
     4741 cggcaccgga cagcgcacga tgcgcaccgg caggctgtcg acatagcggg cgcagaactg
     4801 gcgcccggtc gtcagcacga gcaggctttg cgcgaccatc agcgggatca gcccgaaatg
     4861 cgcgctgcgc accatcacgt cgcggttcag gccgagcgag gccaggtgct cgtcgatcac
     4921 gcccggcgac gcgccgtggg ccccggcgta gaacggcatc ggcgccacgt gctcgcaggc
     4981 gaggtagcgc tcgggcgtcc aggcgcgcgt ggccgcggca cgcacgaccg ggtgctcctc
     5041 ggcgaccagg cagacgatct cgtccgacat cagccggccg aggtgcagct cttcgggcgg
     5101 cttgagccag ttgccgatca ccaggtcgac ctcggcggcg gcaaggctgc gccggtagtc
     5161 gaactcgccc gacagcggca tcagctcgag ccgcatgccg ggcgagacat gcttcaggtg
     5221 cgcgaccagc tcgggcagga acagcgggtc gaggtaatcg ctcgcggcga tgcggaaggt
     5281 gccggcgctg gcggcggggt cgaaaccgcg ctgatgggcg cgcgggctga acaggcggtc
     5341 ggcgtcctgc agcagccgcg tggccgggtc gagcaggcgc aatgcggtgt cggtgggcgc
     5401 catgttgttg ccggcgcgca ccagcaacgg gtcacccgtc agtgcgcgca gccgtttcag
     5461 ctgggcactc acggcgggct gcgtgctttg caggcgcatc gcggcccggg agacactgcg
     5521 ttcggtgatc aaggtcttca acacccggat gaggtagagc tcgatcttgt cggactccaa
     5581 tcgctgtgtc atacgcgccc ctccataggc aatatgcctt tcactgtata ccccggcggg
     5641 cggaagcgac taagtttcgg gtatccgccg actcccgcga gcccgtccat gtccgacccc
     5701 gtgcccagcc gcccgatccg cttcttccac cgcggtgcgg tggtcgaggt cagcgacgcc
     5761 gccgcgaccc gcagcgtgct cgactggctg cgcgaggacg cgcgctgcac cggcaccaag
     5821 gagggctgca acgagggcga ctgcggcgcc tgcaccgtcg tgatcggcga ggccgctgcg
     5881 cccggcgatc cagccgcggt gcgcggcctg agcctgaaga cggtgaacgc ctgcatccag
     5941 ttcctgccga cgctggacgg caaggcgctg ttcacggtcg aagacctgaa ggcgatgaac
     6001 gccgacgcgc tgcaccccgt gcagcaagcg atggtcgagt gccacggctc gcagtgcggc
     6061 ttctgcacgc cgggcttcgt gatgtcgctg tggtcggcct acgagcacgg ccgcgcggcg
     6121 ggttcgcagc cgacgcgcca gcagctcgcc gacgagctgt cgggcaacct gtgccgctgc
     6181 accggctacc gccccatcct cgacgccggc cagcgcatgt tcgacctgcc gccggtggcg
     6241 ctcgacaccg cgccggtgct ggccgcgctg cgcgcgctgg gcagcgacac gagcctgcgc
     6301 tacgtcgccg ccggcagcac cgaacgcttc ttcgcgccga ccacgctcga cgagctcgcc
     6361 gcgttgcgcg aggccaaccc gcaggcccag ctggtcgccg gctcgaccga cgtcgggctg
     6421 tgggtcaaca agcagttccg cgcgctcggc gacatcctct acgtcggcgc ggtcgatgag
     6481 ttgaagcgca tcgaggaacg cgatggcgaa ctgcgcatcg gcgccggcgc ctcgctcgaa
     6541 gcggcattcg cggcgctcgc gaaacgcttc ccggacctga ccgatgtctg gctgcgcttc
     6601 gcgtcgccgc cgatccgcaa cgccggcacg atgggcggca atgtcgcgaa cggctcgccg
     6661 atcggcgatt cgccgccggt gctgatggcg ctggatgcgc agatcgagct gcgccgcggt
     6721 gcgcgcgtgc gccgcctgcc actgcccgag ttctacgtcg actacatgaa gaaccatctc
     6781 gagcccggcg agttcgtgca ggcgatccac gtgccgctga acgggccagc gcgccaggtg
     6841 cgcggctaca agatcagcaa gcgctacgac tccgacatct ctgccgtctg cggcgcgttc
     6901 gcgatcgagt
//
LOCUS       DATMJB010000608         5139 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_707619, whole genome shotgun sequence.
ACCESSION   DATMJB010000608 DATMJB010000000
VERSION     DATMJB010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707619"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..864
                     /locus_tag="VNU48_12865"
     CDS             22..864
                     /locus_tag="VNU48_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin domain-containing protein"
                     /protein_id="HWI12217.1"
                     /translation="MPMTINTNIISLNAQRNLNGSQSSLATSMQRLSSGLRVNSAKDD
                     AAGLAIAERMNAQVRGMNVAVRNANDGISLAQTADGALSSIGDSLQRMRELAVQARNS
                     TNSDSDKDSLNLEYQQLSNEISRVLGGTTFNGKAILGADAGTLTFQVGANVTANDSID
                     VATSDMTADVKITTVTGASIGSTADATALATVIQNIDAALDDINSQRAIYGASQNRFS
                     AVITNLQTSVENQSAARSRIMDADFAAETAAMSRAQILQQAGTAMVAQANTLPQQVLK
                     LLQG"
     gene            1032..2456
                     /gene="fliD"
                     /locus_tag="VNU48_12870"
     CDS             1032..2456
                     /gene="fliD"
                     /locus_tag="VNU48_12870"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HWI12218.1"
                     /translation="MTTSISATGSISSAGIGSGLDVNSIIAGLMKVEQAPLTQLQDQA
                     TAIKTTVSAFGAVQSAMSTFRDAASTLALPSTWNATAATSTDPTAVSVTTTANASAGN
                     YAVQVQSLAASQATVSGTFASADALVGAGTLHFDLGTWNAGATAFTAKAGSSGVDITA
                     SATDTLTTLAAKVNASGAGVTATIVNDASGSRMVFSSSTTGVDNAFRATGLASLAYDP
                     AGGGTGSTRTQAAANASATINGLAVTSATNNLTNVFAGLTITLGKVTTGPVQVAVAQD
                     AATIKKSVQTFVDAYNALSTLLSTDLKYDSATKTAGPLQADSAAVTLQRQLRNMVGAP
                     STASSAFSSISQAGLQFQADGTLKIDDTKLSAALANPIELKKLFTNVDTGNPANNGVA
                     INLRSFGNSALGSDGLLTARISGLNKKLASNQKDQDTLNDRLAATQARLQKQYSALDN
                     QMAGISTLSTYVSQQIANWNKNTA"
     gene            2557..2976
                     /gene="fliS"
                     /locus_tag="VNU48_12875"
     CDS             2557..2976
                     /gene="fliS"
                     /locus_tag="VNU48_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HWI12219.1"
                     /translation="MFATASRSNVSQHQAGVYRQVHVSSGVMGASPHGLIGMLFDGLL
                     GALAEARGAIRSRNIAAKGKAIGRAVRILDEGLSAALDLEQGGTLAADLRALYGYVAL
                     RLTHANLHNDEAALEECVHLIEPLRSGWAAIADHPGT"
     gene            3037..3339
                     /locus_tag="VNU48_12880"
     CDS             3037..3339
                     /locus_tag="VNU48_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HWI12220.1"
                     /translation="MNTALLNYYQAIEKASQDMLEAARVGNWDQVLKLEGACALLISQ
                     LKQTAANEGPLGSEEAQFKSRIMQRILVNDAEIRQLAEPWLDDLDDLLAGRTQTFH"
     gene            3369..4118
                     /locus_tag="VNU48_12885"
     CDS             3369..4118
                     /locus_tag="VNU48_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar regulator YcgR PilZN domain-containing
                     protein"
                     /protein_id="HWI12221.1"
                     /translation="MPMPLDALGAAPGGLDEFRIGAPREVAAVLRQLCDASAPLYLNA
                     RDGSVVNATLWSTDAQRGSVGFTVDPHDPALGALQECGEVVVVGYLDSVKLQFDVGRP
                     VLVHGRQASVLSCSYPREVYRFQRRNAYRVRPLMRAAPVARLRHTDIAEMQLALRVID
                     VSIGGCALFLPADVPPMQVGGVMNQVEIELDADTRFRVNLRLQHVTSLNVDANGVRLG
                     CEFVHADPAALRALQRFIDQTQKRGKLLALE"
     gene            4248..4514
                     /locus_tag="VNU48_12890"
     CDS             4248..4514
                     /locus_tag="VNU48_12890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12222.1"
                     /translation="MSIPSISGVGRVAPSAADTPASAIPSAATTARAGTAAPAVAIDE
                     FTQQPVPPRFPWLSRLSQELESAAKRRPAFAPAPMLGDHVDKSA"
     gene            4611..4907
                     /locus_tag="VNU48_12895"
     CDS             4611..4907
                     /locus_tag="VNU48_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12223.1"
                     /translation="MSPDDFTFLLGAALRDWAEWRARFGTAGWRRKLNNFELSEADAL
                     VERMDRAMRDFMSVQQQQFADATHAARHAAESPEPGFANGFARTPEPSATHAPG"
BASE COUNT          899 a         1885 c         1665 g          690 t
ORIGIN      
        1 gaccaacatg gagtgcccat catgccgatg acgatcaaca cgaacatcat ttcgctgaat
       61 gcccagcgca acctgaacgg gtcgcagtcg tcgctggcga cctcgatgca acgcctgtcc
      121 tccgggctgc gcgtcaactc cgccaaggac gacgccgccg gcctggccat cgccgaacgc
      181 atgaacgcgc aggtgcgcgg catgaacgtc gcggtgcgca atgccaacga cggcatctcg
      241 ctggcgcaga ccgccgacgg cgcgctgtcg tcgatcggcg attcgctgca gcgcatgcgc
      301 gagctcgcgg tgcaggcccg caactcgacc aacagcgact ccgacaagga ctcgctgaac
      361 ctcgagtacc agcagctcag caacgaaatc tcgcgcgtgc tcggcggcac caccttcaac
      421 ggcaaggcga tcctgggcgc cgacgccggc acactgacct tccaggtcgg cgccaacgtc
      481 accgccaacg actcgatcga cgtcgccacc agcgacatga ccgcggacgt gaagatcacc
      541 acggtcaccg gcgcctcgat cggcagcacc gccgacgcca ccgcgctggc cacggtgatc
      601 cagaacatcg acgccgcgct ggacgacatc aacagccagc gcgcgatcta cggcgcctcg
      661 cagaaccgct tctcggcggt gatcacgaac ctgcagacct cggtcgagaa ccagtcggcc
      721 gcgcgcagcc gcatcatgga cgccgacttc gcggccgaga ccgccgcgat gtcgcgcgcg
      781 cagatcctgc agcaggccgg caccgcgatg gtggcgcagg cgaacacgct gccgcaacag
      841 gtgctcaagt tgctgcaggg ctgatcgggc cgcgccggcg cagcgccggc cgagcaccgc
      901 aaaagggcct caagttcggc gcgttcgcgc cgaagaacgg gatgttcggg acttcgctcg
      961 ggcatcccgt tctgcattcc cacgtcggcc ggtgctgcca cgccggcccg acaccgccaa
     1021 ggtagacctc gatgaccacc tcgatcagcg ccaccggttc gatcagctcc gcggggatcg
     1081 gcagcgggct ggacgtgaac tccatcatcg ccggcctgat gaaggtcgag caggcgccgc
     1141 tgacgcagct gcaggaccag gccacggcga tcaagaccac ggtgtcggcc ttcggcgcgg
     1201 tgcagtcggc gatgtcgacc ttccgcgacg cggcctcgac gctggccttg cccagcacct
     1261 ggaacgccac cgcagccacc tccaccgacc cgaccgcggt ctcggtcacg accaccgcga
     1321 acgccagcgc cggcaactat gcggtgcagg tgcagagcct ggcggcctcg caggcgaccg
     1381 tcagcggcac cttcgcgtcg gccgacgcgc tggtcggcgc gggcacgctg catttcgacc
     1441 tcgggacctg gaacgccggt gccaccgcct tcaccgcgaa ggccggcagc agcggcgtgg
     1501 acatcacggc cagcgccacc gacacgctga ccacactggc ggccaaggtc aacgcctcgg
     1561 gcgcaggcgt caccgcgacc atcgtcaacg acgcgagcgg ctcgcgcatg gtgttcagct
     1621 cgagcacgac cggcgtcgac aacgcgttcc gtgccaccgg cctcgccagc ctggcctacg
     1681 acccggccgg cggcggcacc ggcagcacgc ggacccaggc ggccgccaac gccagcgcca
     1741 ccatcaacgg cctcgccgtc acgtcggcga cgaacaacct gaccaatgtc ttcgccgggc
     1801 tgacgatcac gctgggcaag gtcacgaccg gcccggtgca ggtcgcggtc gcgcaggacg
     1861 cggcgacgat caagaaatcg gtgcagacct tcgtcgacgc gtacaacgcg ctgtcgaccc
     1921 tgctcagcac cgacctcaag tacgacagcg ccaccaagac cgccggcccg ctgcaggccg
     1981 acagcgccgc ggtgaccctg cagcgccagc tgcgcaacat ggtgggcgcg ccgtcgacgg
     2041 cgtcgagcgc gttctcgtcg atctcgcagg ccggcctgca gttccaggcc gacggcacgc
     2101 tgaagatcga cgacaccaag ctgagcgccg cgctggccaa cccgatcgag ctgaagaagc
     2161 tgttcaccaa cgtcgacacc ggcaacccgg cgaacaacgg cgtcgcgatc aacctgcgca
     2221 gcttcggcaa ctcggcgctg ggcagcgacg ggctgctgac ggcccggatc agcggcctga
     2281 acaagaagct ggccagcaac cagaaggacc aggacacgct gaacgaccgc ctcgcggcga
     2341 cccaggcgcg cctgcagaag cagtactcgg cactggacaa ccagatggcc ggcatcagca
     2401 cgctgagcac ctacgtgtcg cagcagatcg cgaactggaa caagaacacg gcgtgagcgc
     2461 gtgtggcacg cggtgacgcg gtgcaacatc gctcaagccg gcgccgcgcg gtgccgatat
     2521 ccaaggcatc gcagttccgc ccagagcaag tccaccatgt ttgccaccgc ctcccgctcg
     2581 aacgtttcgc aacaccaggc cggcgtgtac cggcaggtgc atgtctccag cggcgtgatg
     2641 ggcgcctcgc cgcacggcct gatcggcatg ctgttcgacg gcctgctcgg tgcgctggcc
     2701 gaagcgcgcg gcgcgatccg cagccgcaac atcgccgcca agggcaaggc gatcgggcgc
     2761 gcggtgcgca ttctcgacga gggcctgtcg gcggcgctgg acctcgaaca gggcggcaca
     2821 ctcgccgccg acctgcgcgc cctctacggc tacgtggcgc tgcgcctgac gcacgccaac
     2881 ctgcacaacg acgaggccgc gctcgaagag tgcgtccacc tgatcgagcc gctgcgcagc
     2941 ggctgggccg ccatcgccga ccaccccggc acctgaaccg agagccccga catgcgaacc
     3001 gacaccctgc ccagaaccac ggattcgagg gatgccatga ataccgcact gctgaactac
     3061 taccaggcca tcgagaaggc gagccaggac atgctggaag ccgcccgcgt cggcaactgg
     3121 gaccaggtcc tgaagctcga aggcgcctgc gcgctgctga tctcgcagct caagcagacc
     3181 gccgcgaacg aagggccgct gggcagcgaa gaggcacagt tcaagtcgcg catcatgcag
     3241 cgcatcctcg tcaacgacgc cgagatccgc cagctcgcgg aaccctggct cgacgacctc
     3301 gacgacctgc tcgccgggcg cacccagacc ttccactgat ccgatcgagc gcttcggcga
     3361 tggagacgat gccgatgccg ctggacgcgc tgggcgcggc cccgggcggc ctggacgagt
     3421 tccgcatcgg cgcgccacgc gaggtcgcgg cggtgctgcg ccagctctgc gatgccagcg
     3481 cgccgctgta cctcaacgcg cgcgacggca gcgtcgtcaa cgccacgctc tggagcaccg
     3541 atgcgcagcg cggctcggtc ggcttcaccg tcgacccgca cgacccggcg ctgggcgcgc
     3601 tgcaggaatg cggcgaggtc gtcgtcgtcg gctaccttga cagcgtgaag ctgcagttcg
     3661 atgtcggccg gccggtactg gtgcatggcc gtcaggccag cgtgctgagc tgcagctacc
     3721 cgcgcgaggt ctaccgcttc cagcgacgca acgcctaccg cgtgcgcccg ctgatgcgcg
     3781 ccgcgccggt ggcgcgcctg cgccacaccg acatcgccga gatgcagctg gcgctgcgcg
     3841 tgatcgacgt gagcatcggc ggctgtgccc tgttcctgcc ggccgacgtg ccgccgatgc
     3901 aggtcggcgg cgtgatgaac caggtcgaga tcgagctcga cgcggacacg cgtttccgcg
     3961 tcaacctgcg cctgcagcac gtgacctcgc tgaatgtcga tgcgaacggc gtgcgcctgg
     4021 gctgcgagtt cgtgcacgcc gacccggccg cactgcgcgc gctgcagcgt ttcatcgacc
     4081 agacgcaaaa acgcggcaag cttctggcgc tggagtgaac cggcgacgaa gcgcggaaaa
     4141 ggacaacatt tcgacgcata tttcggcgat cgcccctcaa gctttcaggg cagcgcgccg
     4201 atagctcagg catctgccac ttccgcacgg gagcaaggag cgctttcatg agcatcccca
     4261 gcatctcggg tgtcgggcgt gtcgcgccct cggccgctga cacgcctgcg tccgctatcc
     4321 cctccgcagc caccacggcg cgcgcgggca cggccgcgcc ggccgtcgcg atcgacgagt
     4381 tcacgcagca gccggtgccg ccgcgcttcc cgtggctgag ccgcctgtcg caggagctcg
     4441 aatccgccgc aaagcgccgg ccggcattcg ccccggcgcc catgctcggc gaccacgtcg
     4501 acaagtcggc ttgaccggtc cttgccctgc agcgtcgccc tgcggcggca atcggcacgc
     4561 tatgcgcgct accgcacaga ctggcggaac ccggcgccca tactgcactg gtgtcacccg
     4621 acgacttcac ctttctgctc ggcgccgcct tgcgcgactg ggccgaatgg cgtgcccgct
     4681 tcggcacggc cggctggcgc aggaagctga acaacttcga gctgtccgag gccgacgcgc
     4741 tcgtcgagcg catggaccgg gcgatgcggg acttcatgtc ggtgcagcag cagcagttcg
     4801 cggacgcgac gcacgccgcg cgccatgccg ccgagtcgcc cgagccgggg ttcgcgaacg
     4861 ggtttgcgag aacgccggaa ccgtccgcga cgcacgcgcc cggctagtcg cgcaggctac
     4921 acctgcatct gcaccatgcg tcgaggcccg gcgccgcatg gcggcccgtt cggcaacctt
     4981 ttggcaactt cgattttgag cgttgaataa gccgcccgcg acacggcgat gttgcgagcc
     5041 cggcgaacgc gcctgccgtc cttgctcgcg cctctgacac gtgcaggttt gcacggtggt
     5101 gccctcgttg cgcaggccct ctgcctacag atcggttgc
//
LOCUS       DATMJB010000609         2319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_444009, whole genome shotgun sequence.
ACCESSION   DATMJB010000609 DATMJB010000000
VERSION     DATMJB010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444009"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..1621)
                     /locus_tag="VNU48_12900"
     CDS             complement(2..1621)
                     /locus_tag="VNU48_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HWI12224.1"
                     /translation="MSATPASHDVVIMGGGLAGLTLALQLKQRFADLDVLVIERRAHP
                     VPEAAHKVGESSVEIGAHYFSQVLGLQAHLDDAQLRKFGFRFFFSEGRRDIDQVTEIG
                     ASRFLSVPSYQIDRGIFENFLGLEVQRRGVRFVDAAIVRGVELADGGAAHHRLRYEAG
                     GSTQQVSARWLVDACGRAGLLKRKLDLAQPNAHVCNAVWFRIKHRIDIDAWSDDPEWR
                     ARCDPPARWLSTNHLVGAGYWVWLIPLASGSHSVGIVADPRLHPLEGINSFDKAMTWL
                     ETWQPRLFDELDGRRVLLQDFAFFRDFSYGCKQVFSAQRWALTGEAGLFLDPFYSPGS
                     DFIGIANTYITELIAIDRAGRPLAAHAQVYDQIYHSFYESTLALYTDQYPMFGDPEVL
                     PVKVIWDYTYYWGVLAQFFFQRRLTDLAALGALRDELAQCQQLNLAAQAFLRAWSLLS
                     AKRNPAVMLDQAALPWFAELNRGLNDTLDDAQFRARVRASTEQLRVLAAEMLARACAE
                     HPALDASALRTLAGDGAAPVEGSTPLLAEFS"
     gene            complement(1678..>2319)
                     /locus_tag="VNU48_12905"
     CDS             complement(1678..>2319)
                     /locus_tag="VNU48_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HWI12225.1"
                     /translation="RRAPDTVALALEVATQAVAQSGRRADELLSVFTSAHGDLAITDH
                     ICSTLAATPLLLSPTKFHNSVHNAASGYWGIATGCLRASTAVSAFEGSFAAGLLEALT
                     QCAAEAAPVLLVGCDIAACGALVSTNASRGLLAVALVIAPQRSARTLWALDAALVAGA
                     TARIALHSEAARALAGNAMADALPLFEALACGARAPLALPLSATLALKLHLSA"
BASE COUNT          356 a          805 c          851 g          307 t
ORIGIN      
        1 cttacgagaa ctcggccagc agcggtgtcg aaccttcgac cggcgccgcg ccatcgccgg
       61 ccagtgtgcg cagcgccgaa gcatcgagcg ccgggtgctc ggcgcaggcg cgcgccagca
      121 tctcggcggc cagcacgcgc agctgctcgg tcgaggcgcg cacgcgcgcg cggaactgcg
      181 cgtcgtcgag cgtgtcgttc aggccgcggt tcagttcggc gaaccagggc agcgcggcct
      241 ggtcgagcat caccgccggg ttgcgcttgg cgctgagcag cgaccacgcg cgcaggaagg
      301 cctgcgccgc gaggttgagc tgctggcatt gcgcgagctc gtcgcgcagc gcgccgagtg
      361 cggccagatc ggtgagccgg cgctggaaga agaactgcgc cagcacgccc cagtagtagg
      421 tgtagtccca gatcaccttg accggcagca cctcggggtc gccgaacatc gggtactggt
      481 cggtgtacag cgccagcgtg ctctcgtaga acgagtggta gatctggtcg tagacctgcg
      541 cgtgcgccgc gagcggccgg ccggcgcggt cgatcgcgat cagctcggtg atgtaggtgt
      601 tggcgatgcc gatgaagtcg ctgccgggcg agtagaacgg gtcgaggaac agcccggcct
      661 cgccggtcag cgcccagcgc tgcgccgaga acacctgctt gcagccgtac gagaaatcgc
      721 ggaagaacgc gaagtcctgc agcagcacgc gcctgccgtc gagctcgtcg aacaggcgcg
      781 gctgccaggt ctcgagccag gtcatcgcct tgtcgaagct gttgatgccc tccagcgggt
      841 gcaggcgcgg gtcggcgacg atgcccaccg agtgcgagcc cgacgcgagc gggatcagcc
      901 agacccagta gcccgcgccg accaggtggt tcgtcgacag ccagcgggcg ggcgggtcgc
      961 agcgcgcgcg ccattcgggg tcgtcggacc aggcgtcgat gtcgatgcgg tgcttgatgc
     1021 ggaaccacac cgcgttgcag acgtgcgcgt tcggctgcgc cagatcgagc ttgcgcttga
     1081 gcaggccggc gcggccgcag gcgtcgacga gccagcgtgc gctgacctgc tgcgtgctgc
     1141 cgccggcctc gtacctgagg cggtgatgcg ccgcgccgcc gtccgcgagc tcgacgccgc
     1201 gcacgatcgc ggcgtcgacg aagcgcacgc cgcggcgctg cacctcgagg cccagaaagt
     1261 tctcgaagat gccgcgatcg atctggtagc tcggcaccga caggaaacgg ctcgcgccga
     1321 tctcggtgac ctggtcgatg tcgcgccgac cctcggagaa gaagaagcgg aagccgaact
     1381 tgcgcagctg cgcgtcgtcc aggtgagcct gcaggcccag cacctggctg aagtagtggg
     1441 cgccgatctc gaccgaggac tcgccgacct tgtgcgcggc ctcgggcacc gggtgcgcgc
     1501 ggcgttcgat caccagcacg tcgaggtcgg cgaagcgctg cttgagctgc agcgccaggg
     1561 tcagcccggc caggccgccg cccatgatga cgacatcgtg cgaagccggc gttgcgctca
     1621 tggccgcagt atgagcgcaa gcgtcgcagg cggcccggcg ccttacgcgc gctcacatca
     1681 ggccgagagg tgcagcttca gcgccagcgt ggccgacaag ggcagcgcca gcggcgcgcg
     1741 cgcgccgcac gccagcgcct cgaacagcgg caacgcatcg gccatcgcgt tgccggcgag
     1801 cgcgcgcgcc gcttccgaat gcagtgcgat gcgtgcggtc gcgccggcga ccagcgccgc
     1861 gtcgagcgcc cacagcgtcc gcgcgctgcg ctgcggcgcg atcacgaggg ccaccgcgag
     1921 caggccgcgg ctggcgttgg tcgacaccag cgcgccgcag gcggcgatgt cgcagcccac
     1981 cagcagcacc ggcgcggcct cggccgcgca ttgggtcagc gcctccagca acccggcggc
     2041 aaagctgccc tcgaacgcgc tgaccgcggt gctcgcgcgc aggcacccgg tcgcgatgcc
     2101 ccagtagccc gaggccgcgt tgtgcaccga gttgtggaac ttcgtcggcg acagcagcag
     2161 cggcgtcgcc gccagcgtgc tgcagatgtg atcggtgatc gccaggtcgc cgtgcgccga
     2221 tgtgaagacg ctgagcaact cgtcggcccg ccggccggat tgcgcgaccg cctgcgtcgc
     2281 gacctccagc gccagcgcga ccgtgtcggg cgcgcggcg
//
LOCUS       DATMJB010000610         2022 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_677738, whole genome shotgun sequence.
ACCESSION   DATMJB010000610 DATMJB010000000
VERSION     DATMJB010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677738"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VNU48_12910"
     CDS             complement(<1..239)
                     /locus_tag="VNU48_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemoreceptor glutamine deamidase CheD"
                     /protein_id="HWI12226.1"
                     /translation="MPIDRSRAEPPATAPGAAAPSRLNRLKAQPRKPGEASFFFYDAF
                     FKNDAVKILPCEYFVHHEDILIMTTLGSCIAACLW"
     gene            complement(244..1122)
                     /locus_tag="VNU48_12915"
     CDS             complement(244..1122)
                     /locus_tag="VNU48_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HWI12227.1"
                     /translation="MSAAALSFAPARSPAVGATPEGGGEFSFDARDFDRVRALIYARA
                     GISLHAGKQAMVYSRLSRRLRETGHRSFGSYLQWLEAASGAQADEEWQEFINCLTTNL
                     TSFFRESHHFDALLHDLKTRAGQGLRIWCNAASTGEEPYTIAMTVAEALGPGADVKIV
                     ASDIDTKVLATASRGVYPTDARGLTPERQRRHFLRGTGANSGMMRVKPELARMIEFRA
                     HNLMDARWALGEPFDIVFCRNVMIYFDNATQRRVLGQIHAAMRPHGLMYVGHSENFTD
                     VRDLFRLRGKTIYERL"
     gene            1331..1999
                     /locus_tag="VNU48_12920"
     CDS             1331..1999
                     /locus_tag="VNU48_12920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWI12228.1"
                     /translation="MLLLQQAGRELPDRRTVAPVVWMQAVIDALCELSSRDPLTGLSN
                     RRQFELALASEIDRVARAGEPALVLMIDIDHFKQVNDTHGHPAGDLVLKGVAQALQDC
                     IRPMDTLARFGGEEFAMILPNCAPPFGLAVAERVRTKVQQRVVAIAPGQQVQVTVSIG
                     GAFAPQWVRSSAALWISRADQQLYRAKAEGRNRACLEQPTVSLVSAEEKGLLFGTSKF
                     AEFE"
BASE COUNT          303 a          672 c          722 g          325 t
ORIGIN      
        1 tcccacaggc acgcggcgat gcacgagccc agcgtcgtca tgatcaggat gtcctcgtgg
       61 tggacgaagt attcgcaggg caggatcttc accgcgtcgt tcttgaagaa cgcgtcgtag
      121 aagaagaacg aggcctcgcc gggcttgcgc ggctgcgcct tcaggcgatt cagccgcgag
      181 ggcgccgcgg ctccaggcgc ggtcgcaggc ggctcggcgc ggcttcgatc gatgggcatg
      241 gggtcagagc cgttcgtaga tcgtcttgcc gcgcaggcgg aacagatccc gcacgtcggt
      301 gaaattctcg gagtggccga cgtacatcag cccgtgtggc cgcatcgcgg cgtggatctg
      361 gccgagcacc cggcgctggg tggcgttgtc gaagtagatc atcacgttgc ggcagaacac
      421 gatgtcgaag ggctcgccca gggcccagcg cgcatccatc aggttgtggg cgcggaactc
      481 gatcatccgc gccagttccg gcttgacgcg catcatgccg ctgttcgcgc cggtgccgcg
      541 caggaaatgg cggcgctggc gctcgggtgt caggccgcgc gcgtcggtcg ggtagacgcc
      601 gcggctcgcg gtggcgagga ccttggtgtc gatgtcgctg gcgacgatct tcacatcggc
      661 gcccgggccc agcgcctcgg ccaccgtcat cgcgatcgtg tagggctcct cgccggtcga
      721 cgcggcgttg caccagatgc gcaggccctg gccggcacgc gtcttcaggt cgtgcaacag
      781 ggcatcgaag tggtgggatt cgcgaaagaa cgaggtcagg ttggtcgtca ggcagttgat
      841 gaactcctgc cattcctcgt cggcctgggc gccgctggcc gcctcgagcc actgcaggta
      901 gctgccgaac gagcgatgcc cggtctcgcg caggcggcgc gacaggcggc tgtagaccat
      961 cgcctgtttg ccggcgtgca ggctgatgcc ggcccgcgcg tagatcagcg cgcgaacgcg
     1021 gtcgaagtcg cgcgcatcga agctgaattc gccgccaccc tccggcgtcg cgccgacggc
     1081 gggcgatcgg gcgggtgcga acgacagggc ggcggcgctc atgatgtctc cgggtggcag
     1141 aaagggctcg aactcgaccc ggccgcgccg ggccggtgca ccccttcata tcggcgcgcg
     1201 caccgcggcc ttgagcggcc cgctgctaga cttggactca atttttcccc tcgcacgccg
     1261 acatctgttc gtgcactccc agaccgagcc cgcccctgtc gatgctgccc gcttgcagct
     1321 ggcggatgcc atgctgctgc tgcagcaggc gggccgcgag ttgccggacc gccgcaccgt
     1381 cgcgccggtg gtgtggatgc aggcggtgat cgacgcgctg tgcgagctct ccagccgcga
     1441 cccgctgacc ggcctgtcga accggcgcca gttcgagctc gcgctggcca gcgagatcga
     1501 ccgggtcgcg cgcgccggcg agcccgcgct ggtgctgatg atcgacatcg atcacttcaa
     1561 gcaggtaaac gacacgcacg ggcacccggc cggtgacctg gtgctcaagg gcgtcgccca
     1621 ggcgctgcag gactgcatcc gcccgatgga cacgctggcg cgcttcggcg gcgaggagtt
     1681 cgcgatgatc ctgccgaact gcgcgccgcc gttcgggctg gcggttgccg agcgcgtgcg
     1741 taccaaggtg cagcagcgcg tggtcgcgat cgcaccgggc cagcaggtgc aggtcacggt
     1801 cagcatcggc ggcgcgtttg cgccgcagtg ggtgcgctcg tcggccgcgc tgtggatcag
     1861 tcgtgccgat cagcagctct accgtgccaa ggccgaaggt cgcaaccgcg cctgcctcga
     1921 gcagcccacc gtgtcgctcg tcagcgccga ggaaaaaggc ctgctgttcg gcacctcgaa
     1981 gttcgcggag ttcgaatgag cagcagcgaa cccgcagcga cc
//
LOCUS       DATMJB010000611         2652 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_223029, whole genome shotgun sequence.
ACCESSION   DATMJB010000611 DATMJB010000000
VERSION     DATMJB010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223029"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VNU48_12925"
     CDS             <1..247
                     /locus_tag="VNU48_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HWI12229.1"
                     /translation="TYSQFTSVFSAGFWFVPGVVVKLVALALIWAYLHHLIAGLRHLW
                     MDATHAVTLQFGRSSAIFTLAVSVVLTLALGAKLFFF"
     gene            274..636
                     /gene="sdhD"
                     /locus_tag="VNU48_12930"
     CDS             274..636
                     /gene="sdhD"
                     /locus_tag="VNU48_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HWI12230.1"
                     /translation="MAQSFGSKRTVVGAHYGLRDWLSQRVTAALMALFTLAVIVQVLL
                     PGEMGYDRWAGIFAHQWMKLLTFVVIVSLLVHVWVGVRDVLMDYVKSVGARLALQVAT
                     IVWLVGCAGWAIQVLWRL"
     gene            669..2447
                     /gene="sdhA"
                     /locus_tag="VNU48_12935"
     CDS             669..2447
                     /gene="sdhA"
                     /locus_tag="VNU48_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HWI12231.1"
                     /translation="MTAGTAALSKRKFDVVIVGAGGSGMRASLQLSLAGLNVAVLSKV
                     FPMRSHTVAAQGGIGASLGNMSEDNWHYHFYDTVKGSDWLGDQDAIEFMCREAPKVVY
                     ELEHFGMPFDRNPDGTIYQRPFGGHTANYGEKPVQRACAAADRTGHAMLHTLYQQNVK
                     ARTNFFVEWMALDLIRDADGDVVGVTALEMETGEVHILEAKTVLLATGGAGRIFQAST
                     NAFINTGDGLGMAARAGLPLEDMEFWQFHPTGVAGAGVLLTEGRRGEGAILRNSDGER
                     FMERYAPTLKDLAPRDFVSRCMDQEIKEGRGCGPNKDYIVLDMTHLGAETIAKRLPSV
                     LEIGHNFANVDITKEPIPGVPTIHYQMGGVPTNIHGQVVAPKDGDPNAVVNGLYAVGE
                     CSCVSVHGANRLGTNSLLDLLVFGKAAGDHIVATALKAKAHKPLPADAADFTLARLAR
                     LDGSTGGEYAQDVANDIRKSMQQHASVFRTQKLMDAGVVEIKALAERVKGIHLADKSK
                     VFNTARIEALELDNLMETARATMISAEARKESRGAQAREDHPARDDQNWLKHTLWYRD
                     GDRLDYKPVNLKPLSVESFVPKARVY"
     gene            2450..>2652
                     /locus_tag="VNU48_12940"
     CDS             2450..>2652
                     /locus_tag="VNU48_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWI12232.1"
                     /translation="MKIRIQRYDPDKDARPYMQDYDFEPGPADRMLLDALIRIKTSDD
                     SLSLRRSCREGVCGSDAMNINGK"
BASE COUNT          454 a          896 c          881 g          421 t
ORIGIN      
        1 cacttacagc cagttcacgt cggtgttcag cgcggggttc tggttcgttc cgggcgtcgt
       61 cgtcaagctg gtcgcgctgg cgctgatctg ggcctacctg caccacctga tcgccgggct
      121 gcgccacctg tggatggacg ccacgcacgc cgtgaccttg cagttcgggc gttcgtcggc
      181 gatcttcacg ctggccgtga gcgtcgtgct gacgctcgcg ctcggcgcga agttgttctt
      241 cttctaagtc attcatcact gaggcgccga tcaatggcac agagtttcgg ttccaagcgc
      301 accgtcgtcg gcgcgcacta cgggctgcgc gactggctct cgcaacgcgt caccgcggcg
      361 ctgatggcgc tgttcacgct ggcggtcatc gtccaggtgc tgctgcccgg cgagatgggc
      421 tacgaccgct gggcgggcat cttcgcgcac cagtggatga agctgctgac cttcgtcgtg
      481 atcgtctcgc tgctcgtgca tgtctgggtc ggcgtgcgtg acgtgctcat ggactacgtg
      541 aagagcgtcg gcgcccgctt ggcgctgcag gtggcgacga tcgtctggct ggtcggctgt
      601 gccggctggg ccatccaggt tctttggaga ctctgatgca agccgccaat tccccgctgg
      661 ggccccagat gacggccggc acggccgcgc tcagcaagcg caagttcgac gtggtgatcg
      721 tcggtgcggg cggctcgggc atgcgcgcgt cgctgcagct ctcgctcgcc ggcctgaacg
      781 tcgcggtgct gtcgaaggtg ttcccgatgc gctcgcacac cgtcgcggcg caaggcggca
      841 tcggtgcttc gctcggcaac atgagcgaag acaactggca ctaccacttc tacgacaccg
      901 tgaagggctc cgactggctc ggcgaccagg acgcgatcga gttcatgtgc cgcgaggcgc
      961 ccaaggtcgt ctacgagctc gagcacttcg gcatgccgtt cgaccggaat cccgacggaa
     1021 ccatatacca gcgcccgttc ggcggccaca ccgcgaacta cggcgagaag ccggtgcagc
     1081 gtgcctgtgc cgcggccgac cgcaccggcc acgcgatgct gcacacgctg taccagcaga
     1141 acgtcaaggc gcgcaccaac ttcttcgtcg agtggatggc gctcgacttg atccgcgacg
     1201 ctgatggcga cgtggtcggc gtcaccgcgc tcgagatgga gaccggcgag gtccacatcc
     1261 tcgaagccaa gaccgtgctg ctcgccaccg gcggcgccgg ccgcatcttc caggcctcga
     1321 cgaacgcctt catcaacacc ggcgacggcc tgggcatggc ggcgcgcgcc ggcctgccgc
     1381 tggaggacat ggagttctgg cagttccacc cgaccggggt ggccggtgcc ggcgtgctgc
     1441 tgaccgaagg ccgccgcggc gagggcgcga tcctgcgcaa cagcgacggc gagcgcttca
     1501 tggagcgcta cgcgccgacg ctgaaagacc tggcgccgcg cgacttcgtc tcgcgctgca
     1561 tggaccagga gatcaaggaa ggccgcggct gcggaccgaa caaggactac atcgtcctcg
     1621 acatgacgca cctcggcgcc gagacgatcg cgaagcgcct gcccagcgtg ctcgagatcg
     1681 gccacaactt cgcgaacgtc gacatcacga aggagccgat ccccggggtg ccgacgatcc
     1741 actaccagat gggcggcgtg ccgaccaaca ttcacggcca ggtcgtcgcg ccgaaggacg
     1801 gcgacccgaa cgcggtcgtc aacggtctgt atgcggtcgg cgagtgttcg tgcgtcagcg
     1861 tgcacggcgc gaaccggctg ggcaccaatt cgctgctcga cctgctggtg ttcggcaagg
     1921 ccgccggcga ccacatcgtc gccacggcat tgaaggcgaa ggcccacaag ccgctgccgg
     1981 ccgacgcggc cgacttcacg ctggcgcgcc tggcgcggtt ggacggcagc accggcggcg
     2041 agtacgcgca ggacgtggcc aacgacatcc gcaaatcgat gcagcagcac gcgagcgtgt
     2101 tccgcaccca gaaactgatg gacgccggcg tggtggagat caaggccttg gccgagcgcg
     2161 tgaagggcat tcacctcgcc gacaagagca aggtgttcaa caccgcgcgc atcgaggcgc
     2221 tcgagctcga caacctgatg gagaccgcgc gcgccacgat gatctccgcc gaggcgcgca
     2281 aggaaagccg cggcgcgcag gcgcgcgagg accatcccgc ccgcgacgac cagaactggc
     2341 tcaagcacac cctgtggtac cgcgacggcg accggctcga ctacaagccg gtgaacctca
     2401 agccgctctc cgtcgaatcg ttcgttccca aggctcgcgt ctactgacca tgaagatccg
     2461 catccagcgc tacgatcccg acaaggacgc caggccgtac atgcaggact acgacttcga
     2521 gccgggcccg gccgaccgca tgctgctcga cgccctgatc cgcatcaaga cgagcgacga
     2581 ttcgctctcg ctgcggcgct cgtgccgcga gggcgtgtgc ggttccgatg cgatgaacat
     2641 caacggcaag aa
//
LOCUS       DATMJB010000612         3678 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_85130, whole genome shotgun sequence.
ACCESSION   DATMJB010000612 DATMJB010000000
VERSION     DATMJB010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85130"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1031
                     /locus_tag="VNU48_12945"
     CDS             108..1031
                     /locus_tag="VNU48_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HWI12233.1"
                     /translation="MSMKRESLIALIVAPLLLLIGSSAHAALHVFACEPEWGALAQEL
                     GGALVDVSVATSALQDPHQIQAKPSLIARARNADLVVCTGAELEIGWLPVLLQQSGNA
                     KVQAGQPGHFAAADFVRKLDVPTQLDRAQGDVHAAGNPHIQTDPRNIALVAAALGARL
                     QQIDPAHAAEYAKAQAGFAQRWQQAIARWSAQAAPLKGANVVSQHKAFVYLYDWLGLK
                     EVAVLEPKPGVEPTASHLQEVLAALKGTPARMVLYAAYQDSRPSDWLAKNAGIAAVKM
                     PFTVGGTDAAKDLFGLFDDTIARLLAAGTKP"
     gene            1028..1798
                     /locus_tag="VNU48_12950"
     CDS             1028..1798
                     /locus_tag="VNU48_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWI12234.1"
                     /translation="MNWSALDWGILGPAFIAGLLVLSTHVPLGTQVLDRGIVFIDLAI
                     AQIAGLGVIAADAIGLPEGGVAVQLAAVLAALLGAWLLTWTERRAPQQQEALIGVMFI
                     LAACAGILLLAGNPHGGEHLKDLLVGQILWVNKTQLLWLAGLSAALLAALRFGWVERL
                     GRFGFYGAFALAVTASVQLVGVYLVFSSLIIPALGTRGHDRAQRHRIGFGIGAAGYAL
                     GLALSALFDLPSGAVIVWALAACALAFAMTARAGQQRQ"
     gene            complement(1861..2229)
                     /locus_tag="VNU48_12955"
     CDS             complement(1861..2229)
                     /locus_tag="VNU48_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12235.1"
                     /translation="MDRDGLLGHNGVKYNRPGASRQAHVVSENSPMEPTYFAQYRGFE
                     FQCSPERLGTNAFAPRLVIYDAYEAVTLEIPLGVPTPPFDNPTVAAHQAFAHGRRWVD
                     GGYTAAAAAAEGKAPQLLEH"
     gene            2337..2564
                     /locus_tag="VNU48_12960"
     CDS             2337..2564
                     /locus_tag="VNU48_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12236.1"
                     /translation="MKTNVKFLVLLDDAPRALLFDSQQCLLGEVIEEDGFIVESLLRD
                     AKECPVPRNEMLLAIVPPPTPQRPVRCFELH"
     gene            2587..3435
                     /locus_tag="VNU48_12965"
     CDS             2587..3435
                     /locus_tag="VNU48_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWI12237.1"
                     /translation="MATGSRILWKGAISFGLVHVPVSLHTATVEHGIDFDWLDQRSMD
                     PVGYKRINKKTGREIDKSHIVKGVAVEDGHYVVVSDEEIAAAYPQSTQSIDIEAFVAA
                     DAIPFVYLDRPYYLAPIGKGAKVYALLRETLAKTRRIGVARVVIQTKQHLAALVAVGP
                     VLVLNLLRWGADIKPWTDLNLPAEGAKAAGLSEAELKMAAQLVGDMSRPFRPEDFRDS
                     FKDEVMALVHRKVKSGETETVVQPEPVAGAASKSADIIDLADLLKRSLKGRARTSTAA
                     RKRRTG"
     gene            3445..>3678
                     /locus_tag="VNU48_12970"
     CDS             3445..>3678
                     /locus_tag="VNU48_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12238.1"
                     /translation="MATSHVRAEDHHMSAFARGVEPAARGAARYATRVTHAERVIDPA
                     SGITKGELVAYYARVAAPMLPHLKARPVALVRAP"
BASE COUNT          577 a         1255 c         1268 g          578 t
ORIGIN      
        1 cgcccgcatg ggcgccaccg acaaccagct cttcctgcaa taccagatga gcctgggcgc
       61 acacggtgcg cacagctact gaaacgcccg tacgcgaagg agtccgcatg tcgatgaaac
      121 gcgaatccct gatcgccttg atcgttgcac cgctgctgct gctgatcggc agctccgcgc
      181 atgccgcgct gcatgtcttc gcctgcgagc ccgagtgggg cgcgttggca caggaactcg
      241 gtggcgcgtt ggtcgacgtg tcggtcgcca ccagcgcgct gcaggacccg caccagatcc
      301 aggccaagcc cagtttgatc gcgcgcgcac gcaacgccga cctcgtcgtc tgcaccggcg
      361 ccgagctcga gatcggctgg ttgccggtgc tgctgcagca atcgggcaac gcgaaggtgc
      421 aggccggcca gccaggccat ttcgccgcgg cggacttcgt tcgcaagctc gacgtgccga
      481 cccagctcga ccgcgcgcaa ggcgatgtgc atgctgccgg caacccgcac atccagaccg
      541 acccgcgcaa cattgcgctc gtcgccgcgg cgctcggcgc gcgtctgcag cagatcgacc
      601 cggcacacgc ggccgagtac gcgaaggccc aggccggctt cgcgcagcgc tggcagcagg
      661 cgatcgcgcg ctggagcgcg caggccgcgc cgctgaaggg cgcgaacgtg gtgtcgcagc
      721 acaaggcctt cgtctacctc tacgactggc tgggcctgaa ggaggtcgcg gtgctcgaac
      781 ccaagcccgg cgtggagccg accgcatcgc atctgcaaga ggtgctcgcg gcactgaagg
      841 gcacgccggc gcgcatggtg ctctatgcgg cgtatcagga ctcgaggccg tccgactggc
      901 tcgccaagaa cgccgggatc gccgcggtca agatgccgtt cacggtcggt ggcaccgacg
      961 ctgcgaagga cctcttcggc ctgttcgacg acacgatcgc gcgcctgctc gcggccggga
     1021 ccaagccgtg aactggagcg cgctcgactg gggcatcctc ggtccggcat tcatcgccgg
     1081 gctgctggtg ctgtccacgc acgtgccgtt gggcacgcag gtgctggacc gcggcatcgt
     1141 cttcatcgac ctcgcgatcg cccagatcgc cgggctcggc gtgatcgctg ccgacgcgat
     1201 cggcctgccg gaaggcggcg tcgcggtcca gttggcagcg gtcctcgcgg cgctgctcgg
     1261 tgcctggctt ctgacctgga ccgaacgacg cgcgccgcag caacaggagg ccctgatcgg
     1321 tgtgatgttc atcctcgcgg cctgcgccgg catcctgctg ctggccggca atccgcacgg
     1381 cggcgagcat ctgaaggacc tgctggtcgg gcagatcctg tgggtcaaca agacacagct
     1441 cttatggctc gcggggctgt ccgccgcgct gctcgccgcg ttgcgattcg gctgggtcga
     1501 gcggctcggc cggttcggct tctacggcgc gtttgcgctc gcggtcactg cgtcggtgca
     1561 gctcgtcggc gtgtacctgg tgttctcgag cctgatcatt ccggcgctcg gcacgcgggg
     1621 ccatgatcgg gcgcagcgcc atcgcatcgg cttcggcatc ggcgcggcgg gctatgcgct
     1681 cgggctcgcg ctgtcggcgc tgttcgacct gccgtcgggt gcggtgatcg tctgggcctt
     1741 ggccgcctgc gcgctggcct tcgcgatgac cgcacgcgcc ggccaacaga ggcaatgaaa
     1801 aggggcaccc gggggtgccc cttttcttca ttcgcgacag cggccggcag gccgcgccga
     1861 tcagtgttcg aggagttgcg gcgccttgcc ctcggccgca gcggccgccg ccgtgtagcc
     1921 cccatccacc cagcggcggc catgagcgaa cgcctgatgg gcggcgacgg tcgggttgtc
     1981 gaacggcggg gtgggcacgc ccagcgggat ctcgagcgtc accgcttcgt acgcgtcgta
     2041 gatcaccagg cgcggcgcga acgcgttcgt gccgagccgc tcgggcgaac actgaaactc
     2101 gaagcctcga tactgcgcga agtaagtcgg ttccatcggg gagttctcgc taacgacatg
     2161 tgcctggcgc gacgcgccgg gtcggttgta tttgacgcca ttgtggccca gcaacccgtc
     2221 gcgatccacc ccgtggatca gggggcgccg acgcgttcgg ccgtaggcat tttccgacag
     2281 ccgaatccgg cggcgcacac tgggggcgcc gtggtgcgcc gcgtagcatc gcctcgatga
     2341 aaaccaatgt gaaatttctc gtgctgctcg acgatgcgcc acgcgcgctg ctgttcgaca
     2401 gccagcagtg cctgctcggc gaagtcatcg aagaagacgg gttcatcgtc gagagcttgc
     2461 tgcgcgacgc caaggaatgc cccgtgccgc gcaacgagat gctgctcgcg atcgtcccgc
     2521 cgccgactcc gcagcgcccg gtgcgctgtt tcgagttgca ctgaagctga agcgacgagg
     2581 tcggcgatgg ccaccggctc ccgcatcctc tggaaaggcg cgatcagctt cggcctcgtg
     2641 cacgtcccgg tcagcctgca caccgcgacc gtcgaacatg gcatcgactt cgactggctc
     2701 gaccagcgca gcatggaccc tgtgggctac aagcgcatca acaagaagac cggccgcgaa
     2761 atcgacaaga gccacatcgt caagggggtg gcggtcgagg atgggcacta cgtcgtggtg
     2821 agcgacgagg agatcgcggc tgcgtacccg caatcgaccc agtcgatcga catcgaggcc
     2881 ttcgtggcgg cggatgcgat ccccttcgtc tatctcgacc ggccgtacta ccttgcgccc
     2941 atcggcaagg gtgcgaaggt ctacgcgctg ctgcgcgaga cgctggccaa gacgcggcgc
     3001 atcggcgttg cgcgggtcgt gatccagacc aagcagcacc tcgccgcgct ggtggcggta
     3061 ggcccggtgc tggtgctgaa cctgctgcgc tggggtgccg acatcaagcc ctggaccgat
     3121 ctgaacctgc cggccgaggg ggccaaggcc gccggcctca gcgaggccga actgaagatg
     3181 gcagcgcagc tcgtgggcga catgtccagg ccgttcaggc ccgaggactt ccgcgactcg
     3241 ttcaaggacg aggtgatggc gctcgtgcac cgcaaggtca aatcgggcga gaccgagacc
     3301 gtggttcagc cggaaccggt cgctggcgcc gcgtcgaagt cggccgacat catcgacttg
     3361 gccgacctgc tcaagcgcag cctgaagggg cgggcgcgca cgtcgacagc cgcgcgaaag
     3421 cgccgcaccg gctgagagtc cgcgatggcc acttcgcacg tgcgggccga ggaccaccac
     3481 atgagcgcct tcgcacgcgg cgtcgagccg gccgcacgcg gcgccgctcg atacgcgacc
     3541 cgcgtcacgc acgccgagcg tgtgatcgac ccggccagcg gcatcacgaa gggtgaactc
     3601 gtcgcctact atgcgcgcgt ggcggcgccg atgctgccgc acctgaaggc gcgaccggtc
     3661 gcgctggtgc gcgcgccc
//
LOCUS       DATMJB010000613         3519 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_228516, whole genome shotgun sequence.
ACCESSION   DATMJB010000613 DATMJB010000000
VERSION     DATMJB010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228516"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VNU48_12975"
     CDS             complement(<1..438)
                     /locus_tag="VNU48_12975"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWI12239.1"
                     /translation="MNEKAKKIATIGWFATPNKPGDRTLEQQMTGLDKVLAEVAGKTV
                     IDAGCAEGLISMEMAKAGAVKCVGLEVIAGHVELGKALVGDLPVELKVANLNDFDLSA
                     LEPADIVLMLAVLHKLKDPSAVCANLAALAKDLCVIRLPPYGPV"
     gene            complement(431..1174)
                     /locus_tag="VNU48_12980"
     CDS             complement(431..1174)
                     /locus_tag="VNU48_12980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12240.1"
                     /translation="MVTAYPVTGKRKSFDVCLAFARGAGGQIAAAYRPGPAAFYGVDA
                     SNIEIWRQVRANGDDYWYIDNSVFDSARQTYFRVAKNRLQHDGIDVSDGARFRALNIP
                     IAPWRERGEHIVLCPQSESFMRFAGYAGDWTADTVAALRQLTDRDIHVRKWSADKGRL
                     AATLADDLRGAHALVTYSSAAAVTAVLAGVPVVTMWQCVAAPMAGSIEQINNLPMPAR
                     ENWAGVLADREWNLAEMANGTAWKALLNE"
     gene            complement(1168..1968)
                     /locus_tag="VNU48_12985"
     CDS             complement(1168..1968)
                     /locus_tag="VNU48_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12241.1"
                     /translation="MIAINTIRREPHYRRAAFDAGLERLGYKLVTSGPPRSREDLLLM
                     WNLHGGNETLAAEWERLGGTVLVCENGYLGRDELGRQLYAIAAHGHNGSGWFPVGTDG
                     RFEALGIDLAPWRPEGGHMLVAGQRGIGSRSMASPSGWDDRTVARLRDIKVQNVKLRR
                     HPGRTTPETTLEQDLDGASLCVIWSSASGVKALTMGIPVVYCAPHWICEGAASRGLGT
                     ISAPVRDDALRRLAMHRMAYGQWRVEEIEAGAPFRWILEHPHFGSLKW"
     gene            complement(1965..2765)
                     /locus_tag="VNU48_12990"
     CDS             complement(1965..2765)
                     /locus_tag="VNU48_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12242.1"
                     /translation="MKPVPNSVGMIGPGQQFKANDDHAQRLIASGHARFDDGTTPAWD
                     GIRWPGATVVILASGPSLTVEQCEQVQVWCKSAGGAGGAGAEQMRRCIAINTTFRRAP
                     WADVLYACDAPWWRAADDETKRHGFRSHCEEAAAVFRGELWTQDDAARREFRINWVES
                     QRAAGLGKRPGLIHQGGNGGYQAMNLAYHAGAKRLILLGFDMHGTHWHGKYENGLTNT
                     PKHLFQVWIDNFAALARDLTAESVEVVNCSPGSALKCFPAMDLAEALR"
     gene            complement(2777..>3519)
                     /locus_tag="VNU48_12995"
     CDS             complement(2777..>3519)
                     /locus_tag="VNU48_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12243.1"
                     /translation="GKSVDQAAVIDELRKAIDQTLSQLVDERVKSVVDDLPKPKDGES
                     LDSAAVQEMINKAVELVKPKDGDPGRDALQIEPLPAVDVERAYPRGTWAMHAGGMVSS
                     FRATTPGEVCEKHGWQVALNGIAEVAVDLAEDGRTVGVAMSMTDGNVLVKKLHFPVVL
                     DKGVFNYEKEYAKGDGVTWGGSYWISLLDGLKGIEPRGSDTGWRLSIKTGQRGKSAYQ
                     SALDAGFTGTEFEWIKQLSPPDQPTVKL"
BASE COUNT          519 a         1221 c         1143 g          636 t
ORIGIN      
        1 caccgggccg tagggcggca ggcggatgac gcacagatcc ttggcgagag cggcgagatt
       61 ggcgcagact gcggatgggt ccttgagctt gtggagcacc gcgagcatca gcacgatgtc
      121 ggccggctcc agggcgctca agtcgaagtc gttcaggttc gcgaccttca actcgacagg
      181 cagatcgccc accagggcct tgccgagctc cacgtggccg gcgatgacct cgagcccgac
      241 gcacttgacc gcgccggcct tggccatctc catcgagatc aggccctcgg cgcaaccggc
      301 gtcgatcact gtcttgccag cgacctccgc cagcaccttg tcgagcccgg tcatttgctg
      361 ttccagcgtg cggtccccgg gcttgttggg ggtcgcgaac cagccgatcg tcgcgatctt
      421 cttggccttc tcattcattc agtagtgcct tccatgcggt tccgtttgcc atctcggcga
      481 gattccattc gcggtcggcg agcacgccgg cccagttctc acgcgctggc atcggcaggt
      541 tgttgatctg ctcgatgctg ccggccatcg gcgccgccac gcattgccac atcgtcacca
      601 ccggcacgcc ggccagcact gcggtgaccg cagctgctga tgagtacgtg accaaggcgt
      661 gcgcgccgcg caggtcatcg gccaacgtgg cggcgagcct gcccttgtcg gccgaccact
      721 tgcgcacgtg gatgtcgcga tcggtcaact gccgcagcgc ggccacggtg tcagcggtcc
      781 agtcgccggc atagcccgcg aatcgcatga acgattcgga ctgcgggcac agcacgatgt
      841 gctcgccgcg ctcgcgccac ggtgcgatgg ggatattcag cgcgcggaag cgagcgccgt
      901 ccgaaacgtc aatgccgtcg tgttgaaggc ggttcttggc gacgcggaag taggtttgcc
      961 gagccgagtc gaagacagag ttgtcgatgt accagtagtc gtcgccgttg gcgcgcacct
     1021 gccgccagat ctcgatgttc gacgcgtcga cgccgtagaa cgccgccggg ccggggcggt
     1081 aggcagcggc gatctggccg ccggcgccgc gcgcgaaggc aaggcagacg tcgaagctct
     1141 tgcgcttgcc tgtcacgggg taggccgtca ccacttcagg ctcccgaagt gcgggtgctc
     1201 gaggatccag cggaacggcg caccggcctc gatctcttcg actcgccact gcccgtacgc
     1261 catgcggtgc atcgccagcc ggcgcagcgc gtcgtcgcgc acgggtgcgc tgatcgtgcc
     1321 caagccgcgc gacgccgcgc cttcgcagat ccagtgcggc gcgcagtaga cgaccgggat
     1381 acccatcgtc agcgccttga cgccggacgc cgaactccag atcacgcaca ggctggcgcc
     1441 gtcgaggtcc tgctcgagcg tggtctcggg cgtcgtgcgg ccagggtgcc ggcgcaactt
     1501 cacgttctgc accttgatgt cgcgcagccg ggccaccgtg cggtcgtccc acccggacgg
     1561 gctggccatg ctgcggctgc cgatgccgcg ctgcccggcg accagcatgt gcccgccttc
     1621 cggccgccac ggcgcgaggt cgatgcccag cgcttcgaag cgcccgtcag tgccgaccgg
     1681 gaaccagccg ctgccgttgt ggccgtgtgc tgcgatcgcg tagagctgac ggccgagttc
     1741 gtcgcggccg aggtatccgt tctcgcagac cagcaccgtg ccgcccagcc gttcccactc
     1801 ggcggcgagc gtctcgttgc cgccgtgcag attccacatc agcagcaggt cctcgcggct
     1861 gcgcggcggg ccggaggtca ccagcttgta gccgagccgc tcgaggccgg cgtcgaacgc
     1921 cgcgcgccgg tagtgcggct cgcgccggat ggtgttgatc gcgatcaccg cagtgcctcc
     1981 gccagatcca ttgccgggaa gcacttcagc gcgctgcccg gcgagcagtt cacgacctcg
     2041 acgctctcgg cagtcaggtc gcgcgccagc gctgcgaagt tgtcgatcca cacctggaac
     2101 aggtgcttcg gcgtgttcgt cagcccgttc tcgtacttgc cgtgccagtg cgtgccgtgc
     2161 atgtcgaagc ccaacaggat cagccgcttc gcgccggcgt ggtaggccag gttcatggcc
     2221 tggtagccgc cgttcccgcc ttggtggatc aggccgggcc gcttgcccaa gccggccgcg
     2281 cgctggctct cgacccagtt gatgcggaac tcgcggcgcg cggcgtcgtc ctgcgtccag
     2341 agctcgccgc ggaacacggc ggcggcctct tcgcagtggc tcctgaagcc atggcgtttc
     2401 gtttcgtcgt cggcagcccg ccaccacggc gcgtcgcagg cgtagaggac gtcggcccac
     2461 ggcgcacggc ggaacgtggt gttgatggcg atgcacctgc gcatttgctc tgcacctgca
     2521 ccacctgcac cacctgcaga tttgcaccac acctgcacct gctcgcactg ctctaccgtc
     2581 aggctgggcc cgctggccag gatcacgacc gtcgcgccgg gccagcggat gccgtcccat
     2641 gccggcgtcg tgccgtcgtc gaatcgcgcg tggccgctgg cgatcaaccg ctgcgcgtga
     2701 tcgtcgttgg ccttgaattg ctggccaggg ccgatcatgc cgaccgaatt cggcaccggc
     2761 ttcacggcga ccaagttcac agcttgacgg tgggctggtc aggcggactg agttgcttga
     2821 tccactcgaa ctcagttccc gtgaatcctg catctagagc actttggtat gcactcttgc
     2881 ctcgttgccc agtcttgatg ctgagacgcc aacctgtatc gctgccacgc ggctcgatac
     2941 ccttcaatcc gtcaagcaga gaaatccagt acgagccgcc ccatgtcacg ccgtcgccct
     3001 tcgcgtattc cttctcgtag ttgaagacgc ccttgtcgag cacgacaggg aagtgcaact
     3061 tcttcacgag cacgttgccg tcggtcatgc tcatggcgac gccgacggtg cggccgtcct
     3121 cggcaagatc gacggcgacc tcggcgatgc cgttgagcgc gacctgccaa ccgtgcttct
     3181 cacagacctc gcctggtgtg gtggcgcgga acgaactcac catgccgccg gcgtgcattg
     3241 cccaggttcc gcgcgggtag gcgcgctcga cgtcgacggc aggcagcggc tcgatctgca
     3301 gcgcgtcgcg gccgggatcg ccgtccttcg gcttcacgag ctcgacggcc ttgttgatca
     3361 tctcctgcac ggccgccgag tcgaggctct cgccgtcctt gggcttcggc aggtcgtcga
     3421 ccaccgactt gacgcgctcg tctaccagtt gcgacagcgt ctggtcgatg gccttgcgca
     3481 gttcatcaat cactgcggcc tggtcaacac tcttgccgt
//
LOCUS       DATMJB010000614         4061 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_414936, whole genome shotgun sequence.
ACCESSION   DATMJB010000614 DATMJB010000000
VERSION     DATMJB010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414936"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VNU48_13000"
     CDS             <1..1075
                     /locus_tag="VNU48_13000"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWI12244.1"
                     /translation="MAAFGPVVSAAVPLNVTAATLVSLAVSPLNAAVSSFGSQQYAAI
                     GTYTDGSTQDITGTVTWASDTISVATISNAGGSNGLASTTAAGSANISAALGGVNATT
                     ALTVQPLAFTTPGTYAWTVPAGVTSIQVVATGAGGGAFSSPGGNGGVVTATLSVVTGD
                     VLTLNVGAAGAGAGAGAAFGFGTGAGGGSSNVNAGTANQIIAGGGGGAGGGDGGPAFG
                     GDGSSAGTGTGGHGGNSTGGAGGSGGSGGAGGTGGFGGPVGTAGGNGNGGPGGNGGRG
                     AVGGAGVGTGTGGVGGSGCCGGGGGGGYGGGGGGGSGPGADGGGGGGASTGPSGAVYS
                     VATNGGTASAAGGNGSIIITVLP"
     gene            1298..3397
                     /gene="flhA"
                     /locus_tag="VNU48_13005"
     CDS             1298..3397
                     /gene="flhA"
                     /locus_tag="VNU48_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HWI12245.1"
                     /translation="MNPMLQQMQAWLGPNAKAIRSMAAPLVVVMILAMMVLPLPAFAL
                     DLLFTFNIAMALMVMMVAAYMVRPLDFAAFPAVILLTTLLRLSLNVASTRVVLLEGHT
                     GAGAAGAVIESFGHFLIGGNFAVGLIVFAILVVINFIVVTKGAERIAEVGARFTLDAM
                     PGKQMAIDADLNAGLIDDKEAKRRRAEVGEEAEFFGSMDGASKYVRGDAMAGLLILFI
                     NIIGGFVIGVAQHDLSAAQAANSYILLAVGDALVAQIPALLISVAAAMVVSRVGKSED
                     VGSQIARQVFNSPKTLGITAGVIALLGVIPGMPNIVFLLLASGLGYASWWMHRRDKRA
                     AEAPAQRPAEVAQANPEATWDDLMPVDTLGLEVGYRLIALVDKARVAAGGGDLLGRIK
                     GVRKKFAQDVGFLPPPVHIRDNLELKPSAYRLTLRGAVVGEGEAFPGMLLAINPGGAT
                     TQLIGTRTTDPAFGLPAVWIEERQRESAQMAGFTVVDCSTVVATHLSHLMQLHAAKLL
                     GRVETQQLVEHVTKLAPKLIEDVVPKMVGIAALQKVLQLLLEEGVHIRDMRSIVESLA
                     EAAATVTDPAELARRIRIHLAPSIVQQIYGPVRELEVIALEPELERLVTQALNSPHGA
                     ALDPGVADTLAKSAADSAKRQEDLGHPACLLVPDMIRAPMARLLKRAAPRLRVLGHSE
                     IPETHSIRIGSIIGGTA"
     gene            3504..>4061
                     /locus_tag="VNU48_13010"
     CDS             3504..>4061
                     /locus_tag="VNU48_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HWI12246.1"
                     /translation="MNVKRFTARTSRDALILVRQALGEDAVVLSTKPSIDGIEVLAMA
                     PEGMRQIERMAATPPAAPAPAAAPAKSLAARSAARAEPSAAPTEVADDVARLSMSTLS
                     FQDYVRERLLRRRQNAINPRDARAEAPSVPAHHVANERVAAQPAAAMPRAEPEPTPAA
                     APASVLEQRMSDRTPAAAPSLRDVQR"
BASE COUNT          596 a         1431 c         1453 g          581 t
ORIGIN      
        1 catggcggcc ttcggtccgg tggtgtctgc ggcggtgccg ctgaacgtca cggccgcgac
       61 gctggtctcg ctcgccgtgt cgccgctgaa tgcggccgtg tcgagctttg gctcgcagca
      121 gtacgcagcg atcggaacgt acaccgacgg ctcgacgcag gacatcaccg gcacggtgac
      181 ttgggcctcc gacacgatct cggtcgcgac gatcagcaac gctggcggat cgaacggatt
      241 ggccagcacc acggccgccg gctcagcgaa tatttcggca gcgctcggtg gcgtcaatgc
      301 caccacggca ctgaccgtcc agccgctggc gttcaccacg ccgggcacct atgcctggac
      361 cgtgcccgcg ggagtgacgt ccattcaggt cgtggcaacc ggggccggtg gcggcgcctt
      421 ctccagcccc ggaggcaacg gcggggtcgt gaccgccacg ctcagcgtgg tcactggaga
      481 cgtgctcacc ttgaacgtgg gcgcggccgg cgccggcgcc ggcgccggcg ccgccttcgg
      541 cttcggcacc ggcgcgggcg gcggttcgtc caacgtcaat gccggcacgg ccaaccagat
      601 catcgcaggc ggtggtggtg gtgcgggcgg cggtgacggc ggccccgcct tcggcggcga
      661 cggcagcagc gcaggcaccg gcaccggcgg acacggcggc aacagcaccg gcggcgccgg
      721 tggcagcggc ggcagcggcg gcgctggtgg cactggcggc ttcggcgggc cggtcggcac
      781 cgccggcggt aacggcaatg gtggacccgg cggcaacggc gggcggggcg ccgtcggagg
      841 cgcaggcgtc ggcaccggca ccggtggcgt cggcggcagt ggctgctgcg gcggcggggg
      901 tggcggtggt tacggcggtg gcggtggggg gggtagtggc cccggggccg acggcggtgg
      961 tggcggcgga gcaagtacgg gtcccagcgg agccgtgtac tcggttgcaa ccaatggtgg
     1021 tacggcctcc gccgccgggg gcaacggttc aatcatcatc accgtccttc cgtagcacga
     1081 agacagcgat cacgcggcgc ccggcgaagg ctgcgcgcgc cgcgtcgctg acgcggcgga
     1141 gatccgccgt ccgaaggcat gcccgcacgc ggcccagggt ccgaactggc ggcgcttcac
     1201 cccgcttatc cggctcaagt ttccgagccg tcccggaaca atggcgcagc accttccggt
     1261 gcgtctcgcc accccgtttc cggagcttcc gaccgacatg aacccgatgc tgcagcagat
     1321 gcaggcctgg ctcggcccca acgccaaggc gatccgctcg atggccgcgc cgctggtggt
     1381 cgtgatgatc ctggcgatga tggtgctgcc gctccccgcg ttcgcgctgg acctgctgtt
     1441 caccttcaac atcgcgatgg cgctgatggt gatgatggtc gccgcctaca tggtgcggcc
     1501 gctggacttc gcggcgttcc ccgcggtcat cctgctgacg accttgctgc gcctgtcgct
     1561 gaacgtcgcc tcgacgcgcg tcgtgctgct cgagggccac accggcgccg gcgcggcggg
     1621 cgcggtgatc gagtcgttcg gccacttcct gatcggcggc aacttcgcgg tcgggctgat
     1681 cgtgttcgcg atcctggtgg tgatcaactt catcgtcgtc accaagggcg ccgagcgcat
     1741 cgccgaggtc ggcgcgcgct tcacgctgga tgcgatgccc ggcaagcaga tggcgatcga
     1801 cgccgacctg aacgccggcc tgatcgacga caaggaagcc aagcgccgcc gcgccgaggt
     1861 cggcgaggag gccgagttct tcggctccat ggacggcgcc agcaagtacg tgcgcggcga
     1921 cgcgatggcg ggcttgttga tcctgttcat caacatcatc ggcggcttcg tgatcggcgt
     1981 cgcgcagcac gatttgagtg ccgcgcaggc ggcgaacagc tacatcctgc tggcggtcgg
     2041 cgatgcgctg gtcgcgcaga tcccggcgct gctgatctcg gtggccgcgg cgatggtcgt
     2101 ctcgcgcgtc ggcaagagcg aggacgtggg cagccagatc gcgcgccagg tcttcaactc
     2161 gccgaagacg ctgggcatca ccgccggcgt gatcgcgctg ctgggcgtga tcccgggcat
     2221 gcccaacatc gtgttcctgc tgctcgcgtc cggcctcggc tacgcctcgt ggtggatgca
     2281 ccggcgcgac aagcgcgccg ccgaggcgcc ggcgcagcgc cccgccgagg tcgcgcaggc
     2341 gaatcctgag gcgacctggg acgacctgat gccggtcgac accctcggcc tggaagtcgg
     2401 ctaccgcctg atcgcgctgg tcgacaaggc gcgcgtcgcc gcgggcgggg gcgacctgct
     2461 cggccgcatc aagggcgtgc gcaagaagtt cgcgcaggac gtcggcttcc tgccgccgcc
     2521 ggtgcacatc cgcgacaacc tcgaactcaa gcccagcgcc tatcggctca cgctgcgcgg
     2581 cgcggtggtc ggcgaaggcg aggccttccc cggcatgctg ctggcgatca accccggcgg
     2641 cgcgaccacg cagctgatcg gcacccgcac caccgacccc gcgttcggcc tgcccgcggt
     2701 gtggatcgag gagcgccagc gcgagtcggc acagatggcc ggatttaccg tcgttgattg
     2761 ctcgaccgtg gtggcgaccc atctttcaca cttgatgcaa ctccacgcgg ccaaattgct
     2821 gggccgcgtc gagacccagc aactcgtcga gcacgtgacg aagctggccc cgaaactgat
     2881 cgaagacgtg gtccccaaga tggttggcat cgccgcactg caaaaggttc tgcagctcct
     2941 gctcgaagaa ggcgtgcaca tccgcgacat gcgctcgatc gtcgagagcc tggccgaggc
     3001 cgccgccacc gtcaccgacc cggccgagct ggcgcggcgc atccgcatcc acctcgcgcc
     3061 gagcatcgtg cagcagatct acggcccggt gcgcgagctc gaggtgatcg cgctcgaacc
     3121 cgagctcgaa cgcctcgtca cgcaggcgct gaattcgccg catggcgccg cgctcgaccc
     3181 gggcgtggcc gacacgctgg ccaagagcgc cgccgattcc gcgaagcgcc aggaggacct
     3241 gggccacccg gcctgcctgc tggtgcccga catgatccgc gccccgatgg cgcgcctgct
     3301 caagcgcgcc gcgccgcgcc tgcgcgtgct cggccacagc gagattcctg agacacattc
     3361 gatccgcatc ggttcgatca ttgggggcac cgcatgaacc gcgaaagagg ggccccgcgc
     3421 agcggggatc gcagcggttc aggcggtgga gttgccgacc acgccgatct tccgaacact
     3481 caccggcctt ttggaggcaa cgcatgaacg tcaaacgctt taccgcgcgc acctcgcgcg
     3541 acgcactcat tctggtgagg caggccctgg gtgaagacgc ggtggtgctg tccaccaagc
     3601 cttcgatcga cggcatcgag gtgctcgcga tggcgcccga aggcatgcgc cagatcgagc
     3661 gcatggcggc gacgccgccg gccgcaccgg ccccggcggc ggccccggcg aagagcctgg
     3721 ctgcgcgcag cgcggcgcgc gccgagccga gcgccgcacc gacggaagtc gccgacgacg
     3781 tcgcgcgcct gtcgatgagc acgctgtcgt tccaggacta tgtgcgcgag cgcctgctgc
     3841 gccgccgcca gaacgcgatc aacccgcgcg atgcgcgtgc cgaggcgccg agcgtgccgg
     3901 cgcaccacgt tgcgaacgag cgagtcgccg cccagcctgc agcggcgatg ccgcgggccg
     3961 agcccgagcc gaccccggcc gccgcaccgg cgagcgtgct cgagcagcgc atgagcgacc
     4021 gcacgccggc cgccgcgccc tcgctgcgtg acgtgcagcg c
//
LOCUS       DATMJB010000615         4727 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_322739, whole genome shotgun sequence.
ACCESSION   DATMJB010000615 DATMJB010000000
VERSION     DATMJB010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322739"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VNU48_13015"
     CDS             complement(<1..662)
                     /locus_tag="VNU48_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWI12247.1"
                     /translation="MPDRADLSPDDTRFILIGTSHAGNVGAAARAIKVMGFADLVLVA
                     PRFDDVLRQADALAMASGATDVLERARVVPTLADALEGVTYACATAMTPRDFGPPTRA
                     PRELFGELATGAHRVAFVFGSERFGMSNDDVYRCHVCLSIPTVAEYGSLNLAQAVQLL
                     AYDWRQALGGFPVQPRTHDARRADAVAVQGVLDHWQRTLVDIDFLDPAVPRKLMPRLQ
                     QLL"
     gene            773..1819
                     /locus_tag="VNU48_13020"
     CDS             773..1819
                     /locus_tag="VNU48_13020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWI12248.1"
                     /translation="MSQALHPMLNTAIKAARSAGAIINRASLDLDLLKVNTKSPNDFV
                     TEVDTKAEAIIIETLLGAYPGHGILAEESGRAHGAKDSEFVWIIDPLDGTTNFIHGFP
                     VYSVSIALAFRGQVQQAVVYDPTRNDLFYASKGRGAFLNDRRLRVSKRARLAECLIGT
                     GFPFRKGDNFKRYLQMMELMMPACAGLRRPGSAALDLCYVAAGYTDGFFETGLSPWDI
                     AAGALLVTEAGGLIGNFTGESDYLYQREIVAGSPKIYGQLVAMLGPFSRVIAPDAPTA
                     ADTTLGPTPEDALAATVAIAPDPDAPTKAPAPVKKPATRISKESMAREKQATADVDRK
                     PPRARAPRGSDTPF"
     gene            1917..2834
                     /locus_tag="VNU48_13025"
     CDS             1917..2834
                     /locus_tag="VNU48_13025"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWI12249.1"
                     /translation="MSQPYIPAFTLEPTKRAVETNQAAAASKPTPRFIDDVAAGRVVL
                     PTIPHVVQRLITALRDPQIDVRTIGAALSRDPVLSAKVLRVANSSFFGGRRSMASIDA
                     AVALIGTQALHRLVVACGVSSTFEGVPGIDMRTFWRDGLVAATAAHKLAPRLAADPEE
                     AYLCGLLHATGHLILCRSYPEIANAMFTGFAVVRGAELAAIELEAFGIDHPTVGALWV
                     ESLGFPRSVVDAIGHTAQPAADSDGPLDLALRSACSLAAAVAQGDAAETALSALAPKV
                     RALFAGPDDKPDAAFDKLYEALQQADPTF"
     gene            2994..>4727
                     /gene="mutS"
                     /locus_tag="VNU48_13030"
     CDS             2994..>4727
                     /gene="mutS"
                     /locus_tag="VNU48_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009931111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWI12250.1"
                     /translation="MNKSLSHNAASDPAFAETPNHTPMMQQYLRIKADFPETLVFYRM
                     GDFYELFFDDARKANRLLDITLTARGTSGGEPIPMAGVPVVSVDGYLAKLIRMGEAVA
                     ICEQIGDVATAKGPVERKVVRVVTPGTVTDTELLADKSDALLLAVAKQGKGFGLAWLA
                     LTRGELGLTECSEAELGAWLARLAPAEVLVDRDQVPAALEQARITVTHRPAWQFDTAL
                     GARKLCEQLRVASLAGFNAQDLRAAQAAAAALLSFAEHTQGQALAHVRRLSVQRASDL
                     LTLPPTTHRNLELTQTLRGEDAPTLLSLIDTCRTGMGSRALRHWLTHPLRERRAATQR
                     HDAIEALLAARLSELRDALRGISDVERITARVALRQVRPRELTGLRATLQALPALRAQ
                     LPPGEAALLDMLGEALAPPDEILALLGAAIAAEPAVLLRDGGVIAPGFDAELDELRGI
                     GQNCDAYLIELEARERARTGIANLRVQFNKVHGFYIEVTTSQAGKVPSDYQRRQTLKN
                     AERFITPELKSFEDKALSAQDRALARERWLYERLLDGLQAHLEPLSALARALAALDAL
                     AALAERASSLGW"
BASE COUNT          715 a         1776 c         1598 g          638 t
ORIGIN      
        1 agcagctgct gcagccgcgg catcagcttg cgcggcaccg cggggtcgag gaagtcgatg
       61 tcgaccagcg tgcgctgcca gtgatcgagc acgccctgca ccgcgacggc gtcggcacgc
      121 cgcgcatcgt gcgtgcgcgg ctgcaccgga aagccgccca gcgcctggcg ccagtcgtag
      181 gccagcagct gcaccgcctg cgcgaggttc agcgaaccgt actcggccac cgtcgggatg
      241 ctcaggcaga cgtggcagcg gtagacatcg tcgttgctca tgccgaagcg ctcggagccg
      301 aacacgaacg cgacccggtg cgcacccgtc gccagctcgc cgaacagttc gcgcggcgcg
      361 cgtgtcggcg gcccgaagtc gcgcggcgtc atcgcggtcg cgcaggcgta cgtgacgccc
      421 tccagcgcat cggccagcgt cggcacgacg cgggcgcgct cgagcacgtc ggtcgcgccg
      481 ctggccatcg cgagcgcgtc ggcctggcgc agcacgtcgt cgaagcgcgg cgcgaccagc
      541 acgagatcgg cgaagcccat caccttgatc gcgcgcgccg ccgcgccgac attgccggcg
      601 tggctggtgc cgatcaggat gaagcgggtg tcatctggcg acaggtcggc gcggtccggc
      661 acggctgggg ctcgcaggta aaatggcgat tatccgcagc ggctcgccgc ggcccgctct
      721 ttgtccctgc cagcccggcc gcccggcccc gatccctcgc gataggtcac ccatgtccca
      781 agccctgcac cccatgctga acaccgcgat caaggcggcc cgcagcgccg gggcgatcat
      841 caaccgggcc tcgctcgacc tggacctgct gaaggtcaac accaagtcgc cgaacgactt
      901 cgtcaccgag gtcgacacca aggccgaggc gatcatcatc gagacactgc tcggcgccta
      961 ccccggccac ggcatcctcg ccgaagaatc cggccgcgcg cacggcgcga aggacagcga
     1021 gttcgtctgg atcatcgacc cgctcgacgg caccaccaac ttcatccatg gcttcccggt
     1081 ctactcggtc tcgatcgcgc tcgcgttccg cggccaggtg cagcaggcgg tcgtctacga
     1141 cccgacgcgc aacgacctgt tctatgcgtc gaaggggcgc ggcgcgttcc tgaacgaccg
     1201 gcggctgcgc gtgtccaagc gcgcgcgcct ggccgagtgc ctgatcggca ccggcttccc
     1261 gttccgcaag ggcgacaact tcaagcgcta cctgcagatg atggagctga tgatgccggc
     1321 ctgcgccgga ctgcgccgcc cgggctcggc cgcgctcgac ctgtgctacg tcgcggccgg
     1381 ctacaccgac ggcttcttcg agaccggcct gtcgccgtgg gacatcgccg ctggcgcgct
     1441 gctcgtcacc gaggccggtg gcctgatcgg caacttcacc ggcgagtccg actacctcta
     1501 ccagcgcgag atcgtcgccg gcagcccgaa gatctacggc cagctcgtcg cgatgctcgg
     1561 cccgttctcg cgcgtgatcg cccccgacgc gccgaccgcc gccgacacga cgctcggtcc
     1621 gacaccggaa gacgcgctcg ccgccacggt cgcgatcgca cccgaccccg acgcgccgac
     1681 gaaggcaccg gcgccggtca agaagcccgc cacacgcatc agcaaggaga gcatggcgcg
     1741 cgagaagcag gccacggccg acgtcgatcg caagccaccg cgtgcgcgcg cgccgcgcgg
     1801 gagcgacacg ccgttctgag cgactccgcg ccgcgcgccg cgtcatgccg acgtgtcgtc
     1861 gttcggcgta tcgtgacact tgcgtcattg ccgtggatct gccgaaaggt tccgacatga
     1921 gtcaacccta catccccgca ttcacgctcg agccgacgaa gcgagccgtc gagacgaacc
     1981 aggccgcagc cgcgtccaaa cccacgcccc gcttcatcga cgacgtggcc gccggtcgcg
     2041 tcgtgctgcc cacgatcccg cacgtcgtgc agcgcctcat cacggcgctg cgcgacccgc
     2101 agatcgatgt ccgcacgatc ggcgcggccc tgtcccgcga tccggtgctc agcgcgaagg
     2161 tgctgcgcgt cgcgaactcg tcgttcttcg gcggccggcg ctcgatggca tcgatcgacg
     2221 ccgccgtcgc gctgatcggc acccaggcgc tgcaccggct cgtcgtcgcc tgcggggtct
     2281 cgtcgacgtt cgaaggcgtc ccgggcatcg acatgcggac cttctggcgc gacggcctgg
     2341 tggccgccac cgccgcccac aagctggcgc cacgcctcgc ggccgacccg gaggaggcct
     2401 acctctgcgg cctgctgcac gccaccgggc acctgatcct gtgccgcagt tacccggaga
     2461 tcgcgaatgc catgttcacc gggttcgcgg tggtccgcgg cgccgagctc gccgcgatcg
     2521 aactcgaagc cttcggcatc gaccacccga cggtcggcgc gctgtgggtc gagtccctcg
     2581 gctttccgcg ctcggtcgtg gacgcgatcg gccacaccgc gcagcctgcg gccgacagcg
     2641 acgggccgct cgatctcgca ctgcgcagcg cctgttcgct ggcggcggcg gtcgcgcagg
     2701 gcgacgctgc cgagaccgca ctgagcgcgc tggcgcccaa ggtgcgcgcc ctgttcgccg
     2761 gccccgacga caagcccgac gctgcgttcg acaagctcta cgaggcgctg cagcaggccg
     2821 atccgacgtt ctgagtgaag atcgcgcacg gaatctgccc ttcccccctc catgcccgct
     2881 cccgacggtc tgcccacgcc ggcgcgccga tgcgccgcgc gggcggcgcc agcggcatgc
     2941 tgggtacggc ccgaccgata atcacgcaca agtacccgat gcgtacggag tcgatgaaca
     3001 agtccttgag ccacaacgcc gcttccgacc cagccttcgc ggagactccg aaccacacgc
     3061 cgatgatgca gcagtacctg cgcatcaagg ccgacttccc cgagacactc gtgttctatc
     3121 gcatgggcga cttctacgag ctgttcttcg acgacgcgcg caaggcgaac cggctgctcg
     3181 acatcacgct gacggcacgc ggcacgagcg gcggcgagcc gatcccgatg gccggcgtgc
     3241 cggtggtttc ggtcgacggc tacctcgcca agctgatccg catgggcgag gcggtcgcga
     3301 tctgcgagca gatcggcgat gtcgccaccg cgaaggggcc ggtcgagcgc aaggtggtgc
     3361 gcgtcgtcac gccgggcacc gtcaccgaca ccgaactgct ggccgacaag agcgacgcgc
     3421 tgctgctcgc ggtcgcgaag cagggcaagg gcttcggcct ggcgtggctg gcgctgacgc
     3481 gcggcgagct cggcctgacc gagtgcagcg aggccgagct cggcgcctgg ctcgcgcggc
     3541 tcgcgccggc cgaggtgctg gtcgatcgcg accaggtgcc ggcggcgctg gaacaggcgc
     3601 gcatcacggt cacgcatcgg ccggcctggc agttcgacac cgcgctgggc gcgcgcaagc
     3661 tgtgcgagca gctgcgcgtc gcgagcctgg ccggcttcaa cgcgcaggac ctgcgcgccg
     3721 cgcaggccgc cgcggccgcg ctgctcagct tcgccgagca cacccagggc caggcgctcg
     3781 cgcacgttcg gcgcctgagc gtgcagcgcg ccagcgacct tctgacgctg ccgccgacga
     3841 cgcaccgcaa cctggagctg acgcagacgc tgcgcggcga ggacgcgccg acgctgctgt
     3901 cgctgatcga cacctgccgc accggcatgg gctcgcgggc gctgcgccac tggctcacgc
     3961 accccttgcg cgaacgccgc gccgcaacgc agcgccacga tgcgatcgag gccttgctcg
     4021 cggcgcgcct gagcgaactg cgcgacgcat tgcgcggcat cagcgatgtc gagcgcatca
     4081 ccgcacgcgt cgcgctgcgc caggtgcggc cgcgcgaact gaccggcctg cgcgcgaccc
     4141 tgcaggcgct gcccgcgctg cgcgcgcagc tgccgcccgg cgaggccgcg ctgctcgaca
     4201 tgctcggcga ggcgctcgca ccaccggacg agatcctcgc actgctcggc gccgcgatcg
     4261 ccgccgaacc cgcggtgctg ctgcgcgacg gcggcgtgat cgcgcccggc ttcgacgccg
     4321 agctcgacga actgcgcggc atcggccaga actgcgatgc gtacctcatc gaactcgagg
     4381 cgcgcgaacg cgcgcgcacc ggcatcgcga atctgcgggt tcaattcaac aaggtgcatg
     4441 gcttctacat cgaggtgacg accagccagg cgggcaaggt gccaagcgac taccagcgcc
     4501 gccagaccct gaagaacgcc gagcgcttca tcacccccga gctgaagagc ttcgaggaca
     4561 aggcgctgtc ggcgcaggac cgcgcgctgg cgcgcgagag gtggctctac gagcggctgc
     4621 tcgacggcct gcaggcgcat ctcgagccgc tctcggcgct ggcccgcgcg ctggccgcgc
     4681 tggacgcgct cgccgcgctg gccgagcgcg cctcgagcct gggctgg
//
LOCUS       DATMJB010000616         3884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_588610, whole genome shotgun sequence.
ACCESSION   DATMJB010000616 DATMJB010000000
VERSION     DATMJB010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588610"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1609)
                     /gene="ubiB"
                     /locus_tag="VNU48_13035"
     CDS             complement(62..1609)
                     /gene="ubiB"
                     /locus_tag="VNU48_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HWI12251.1"
                     /translation="MRHIARLLVICVTVLRYGLDELALSSFRQRWVRALVRVMTIGRT
                     LDAPRGERLRLGLERLGPIFVKFGQVLSTRRDLLPADLADELARLQDRVPPFPAEHAR
                     ALVEQAFGKPIAAIFTRFDAEPVASASIAQVHFAQLKDGREVAVKVLRPGMLEVIDDD
                     LSLMRTLARWVERLSADGKRLKPREVVAEFANYLHDELDLVREAANAAQLRRNMHDLD
                     LIMIPEMVWDLCTQSVIVMERMNGVPISQTQRLLDAGVDIPKLARDGVTIFFTQVFRD
                     GFFHADMHPGNIQVSIAPESFGRYIALDFGIVGTLTEIDKEYLAQNFIAFFRRDYKRV
                     AELHVESGWVPPATRVDELEGAIRAVCEPHFDRPLKDISLGQVLLRLFQTSRRFNVEI
                     QPQLVLLQKTLLNVEGLGRQLDPELDLWNTAKPFLEQWMDKQVGWAGFLEGLRREAPR
                     YVQLLPELPRLIHQALQPRAAAEQRLLETLLVEQRRMARLLRAIVCVACGVALGLLAS
                     HLWLNTH"
     gene            complement(1755..2270)
                     /locus_tag="VNU48_13040"
     CDS             complement(1755..2270)
                     /locus_tag="VNU48_13040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12252.1"
                     /translation="MFHSLSSLLGTAAIERLTLFVNHLLASEPIAADRLRAHAGRCMQ
                     LHFEGWPTLLPPLPPTAFRVTPAGLIEWCGTELPTEPDLRVGIDASNPALALAQALAG
                     TRPKVEIAGDAAFATDLNWLFDNLRWDVQDDLAGIVGAAPAREITRIAGGIAAGLREA
                     ANTLSGLVTRR"
     gene            2507..>3884
                     /locus_tag="VNU48_13045"
     CDS             2507..>3884
                     /locus_tag="VNU48_13045"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HWI12253.1"
                     /translation="MFEDRTYKRTFYSAPQSVTSFVAAFLEPSITVLTFLFATVYADE
                     WRPNDRPALTLCLLVFALTFPGRNRFRDNLIAAGVDILSSWVTLLAILALCGYATNSQ
                     RYFEDDVLLIWAIATPVLQWLAVWLGKVVTQRLSARATARRTSIVVGAGALGVKVAKA
                     LNESADYGVDFIGYFDDRTDDRLHQEASAQRLGGLKDVAAYVTRHGVREVYITLPLGS
                     QPRIVELLEDVQGTTASLFFVPDVFGISIIQGRLQDMNGVPVVGICETPFTGTNELVK
                     RISDLVLATLILILISPLLLVIAIGVKLSSPGPVVFRQRRNGLDGSEIVIYKFRSMKS
                     QDNGPMVRQATKADPRITAFGALIRRTSMDELPQFINVLQGRMSIVGPRPHAVAHNEE
                     YRRLIKAYMVRHKVKPGITGWAQVNGHRGETDTIEKMQARVEYDLEYLRNWSLGLDLQ
                     IIVRTIRLV"
BASE COUNT          610 a         1351 c         1339 g          584 t
ORIGIN      
        1 aaaaggaaaa cctcgaatta tacggcgccg ggccgagttc gcgctgcgcc gggtcgcagg
       61 ctcagtgggt gttcagccac aggtgcgacg ccagcagccc cagcgccacc ccgcaggcga
      121 cgcagacgat cgcgcgcagc agccgcgcca ttcgtcgctg ctcgacgagc agcgtctcca
      181 gcaggcgctg ctcggccgcc gcgcgcggct gcagggcctg gtggatcagg cgcggcagct
      241 cgggcagcaa ctgcacgtag cgcggtgcct cgcgccgcag cccttcgagg aacccggccc
      301 agccgacctg cttgtccatc cactgctcga ggaagggctt ggcggtgttc cacagatcga
      361 gctcggggtc gagctggcgg cccaggccct cgacgttgag cagcgtcttc tgcagcagca
      421 ccagttgcgg ctggatctcg acgttgaagc ggcgcgaagt ctggaacaaa cgcagcagca
      481 cctggccgag cgagatgtcc ttcagcggcc ggtcgaagtg cggctcgcag acggcgcgga
      541 tcgcgccctc gagctcgtcg acgcgcgtgg ccggcggcac ccagcccgac tcgacatgca
      601 gctcggccac ccgcttgtag tcgcgccgga agaacgcgat gaagttctgc gccaggtatt
      661 ccttgtcgat ctcggtgagc gtgccgacga tgccgaagtc cagcgcgatg tatcggccga
      721 agctctcggg cgcgatgctg acctggatgt tgcccgggtg catgtcggcg tggaagaagc
      781 cgtcgcggaa cacctgcgtg aagaagatcg tgacgccgtc gcgcgccagc ttcggaatgt
      841 cgacgccggc atcgagcagc cgctgcgtct ggctgatcgg cacgccgttc atgcgctcca
      901 tcacgatcac gctctgggtg cacaggtccc agaccatctc ggggatcatg atcaggtcga
      961 ggtcgtgcat gttgcggcgc agctgcgcgg cattcgcggc ctcgcgcacc agatcgagct
     1021 cgtcgtgcag gtagttcgcg aactcggcga cgacctcgcg cggcttcaga cgcttgccgt
     1081 cggcgctcag gcgctcgacc cagcgggcca gcgtgcgcat cagcgacagg tcgtcgtcga
     1141 tgacctcgag catgcccggg cgcagcacct tgacggcgac ttcgcggccg tccttcagct
     1201 gcgcgaagtg cacctgcgcg atcgacgcgc tggccaccgg ctcggcgtcg aagcgcgtga
     1261 agatcgcggc gatcggtttg ccgaacgcct gctcgacgag cgcgcgcgcg tgctcggccg
     1321 ggaacggcgg cacgcggtcc tgcagcctgg cgagctcgtc ggccaggtcg gcgggcagca
     1381 ggtcgcgccg cgtcgacagc acctggccga acttgacgaa gatcggaccg agccgctcca
     1441 gcccgagccg cagccgctcg ccgcgcggcg cgtcgagcgt gcggccgatg gtcatcaccc
     1501 gcaccagcgc gcgcacccag cgctgccgga agctggacag cgcgagttcg tcgagcccgt
     1561 agcgcagcac cgtcacgcag atgacgagca gccgcgcgat gtgcctcaat ggggcccctc
     1621 gtcgaacggg tacgttcgcc gatccccttg ggccacgaag gggcccctgc gtggcccttg
     1681 gatgcgggcc ggccgaggtc cacacggtgg gcctcttcgc ggcccatggc ggcccggcgc
     1741 tcggcccgcg cgcctcatcg ccgcgtcacc aggccgctca aggtgttcgc ggcctcgcgc
     1801 aggcccgccg cgatgccgcc ggcgatgcgc gtgatctcgc gcgccggcgc cgcgccgacg
     1861 atgccggcca ggtcgtcctg cacatcccag cgcaggttgt cgaagagcca gttcaggtcg
     1921 gtcgcgaacg cggcgtcgcc ggcgatctcg accttcgggc gcgtgccggc cagcgcctgc
     1981 gccagcgcca gcgccggatt cgaggcgtcg atgccgacgc gcagatcggg ctcggtcggc
     2041 agctccgtgc cgcaccactc gatcaggccg gccggcgtga cgcgaaaggc cgtcggcggc
     2101 aagggcggca acagtgtcgg ccagccctcg aagtgcaact gcatgcaacg tccggcatgg
     2161 gcgcgcaggc ggtcggcggc aatcggctcc gaggccagca ggtggttgac gaagagcgtc
     2221 aggcgctcga tcgcagcggt gccgagcagc gaactgagac tgtgaaacat gcacggattg
     2281 taggcacggc ggcctcgagc tccctcgact ccggcggccg gcgcgcctgc gtttttgatg
     2341 gagaatgccc cggcacgtcg ccgaaccgga cgtgcgggag gcatgcgccg taccggtacg
     2401 ccggcagtgt gcgtgcgttg ctgcgctccc gcacggccgc cggcgacgaa ccgcacgccg
     2461 gtgcaccgtc ccgcgacggt cgggcgtgcg ggcacaccct gaacccatgt ttgaagaccg
     2521 cacctacaag agaacgttct acagcgcgcc gcaatccgtg acgtcgttcg tggcggcgtt
     2581 cctggagccc agcatcacgg tgctgacctt cctgttcgcg acggtctatg ccgacgagtg
     2641 gaggccgaac gatcgcccgg cgctgacgct gtgcctgctg gtgttcgcgc tgacctttcc
     2701 cggccgcaac cgcttccgcg acaacctgat cgccgccggc gtcgacatcc tgtcgtcgtg
     2761 ggtcacgctg ctggcgatcc tggcgctgtg cggctatgcg acgaacagcc agcgctactt
     2821 cgaggacgac gtgctgctga tctgggcgat cgccacgccg gtgctgcagt ggctcgcggt
     2881 ctggctcggc aaggtcgtca cgcagcggct gtcggcgcgc gccacggcgc ggcgcacgtc
     2941 gatcgtcgtc ggcgccggcg cgctgggcgt gaaggtcgcc aaggccctga acgaaagcgc
     3001 cgactacggc gtcgacttca tcggctactt cgacgaccgc accgacgacc gcctgcacca
     3061 ggaggccagc gcgcaacgcc tgggcggcct gaaggacgtg gccgcctacg tcacccggca
     3121 cggcgtgcgc gaggtctaca tcacgctgcc gctgggctcg cagccgcgca tcgtcgagct
     3181 gctcgaggac gtgcagggca cgaccgcctc gctgttcttc gtgcccgacg tgttcggcat
     3241 cagcatcatc cagggccggc tgcaggacat gaacggcgtg ccggtcgtcg gcatctgcga
     3301 gacgccgttc accggcacca acgaactcgt caagcgcatc agcgacctgg tgctggccac
     3361 gctgatcctg atcctgatct cgccgctgct gctggtgatc gcgatcggcg tgaagctcag
     3421 ttcgcccggc ccggtggtgt tcaggcagcg ccgcaacggc ctggacggca gcgagatcgt
     3481 gatctacaag ttccgctcga tgaagtcgca ggacaacggc ccgatggtgc gccaggccac
     3541 caaggccgat ccgcgcatca ccgccttcgg cgccctcatc cgccgcacct cgatggacga
     3601 actgccgcag ttcatcaacg tgctgcaggg ccggatgagc atcgtcggcc cgcgcccgca
     3661 cgcggtggcg cacaacgagg aataccggcg cctgatcaag gcctacatgg tgcgccacaa
     3721 ggtcaagccc ggcatcaccg gctgggcgca ggtgaacggc caccgtggcg agaccgacac
     3781 gatcgagaag atgcaggcgc gcgtcgaata cgatctcgaa tacctgcgca actggtcgct
     3841 gggtctggac ctgcagatca tcgtgcgtac gatccgcctg gtgt
//
LOCUS       DATMJB010000617         4230 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_519499, whole genome shotgun sequence.
ACCESSION   DATMJB010000617 DATMJB010000000
VERSION     DATMJB010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519499"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..134)
                     /locus_tag="VNU48_13050"
     CDS             complement(<1..134)
                     /locus_tag="VNU48_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HWI12254.1"
                     /translation="MTYPKELPLASLQFYATAPYPCSYIEGRQARSQVATPSHLIHAD
                     A"
     gene            complement(131..874)
                     /gene="aat"
                     /locus_tag="VNU48_13055"
     CDS             complement(131..874)
                     /gene="aat"
                     /locus_tag="VNU48_13055"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HWI12255.1"
                     /translation="MRGPDDPLPATGAALPLGSDAPGLLAAGGHPTPQRLTEAYAKGV
                     FPWYSEGQPVLWWSPDPRMVLFTDEFRLSRSLRKAVRRFAASTRCELRIDSAFRRVIT
                     ACAQTPREGQDGTWIVPEMIEAYCDWHALGRAHSFETWVDGELVGGLYGIGLGHMFFG
                     ESMFAHRTDASKIAFAALVCFCREHRVPMIDCQQHTRHLESMGARELRRPEFEARLAH
                     SLGEPPITDWTYDLRLWRHLGPAAEEPGP"
     gene            987..1772
                     /locus_tag="VNU48_13060"
     CDS             987..1772
                     /locus_tag="VNU48_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HWI12256.1"
                     /translation="MDSRFRSIGSAAALAALTLPTGAAQAADGDGFAPITPYRPSVSS
                     PAQLPAPGQIELEFGGLRQRSDDARHSSLPYLFKLAFDASWGLLLGGEARVWQRDGGA
                     RAQGLGDTSVVLKRAWLVDEGSAFGVELGAKLPTANDTIGSGHADYSINTIYSRDFGA
                     LHMDANLNALRFGRVDAGTSRTQWGASASFSTALAEHWGATGELSGTRRTGVDHGAQL
                     LAALAYSPSKFLTIDFGIARSPRPAPATTSLFAGVVFPLARLW"
     gene            complement(1776..2558)
                     /locus_tag="VNU48_13065"
     CDS             complement(1776..2558)
                     /locus_tag="VNU48_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HWI12257.1"
                     /translation="MKLQDKVCIVTGAASGIGKEIALTYAREGGKVVIADMNKAAAQE
                     AADEIVKAGGTAMAVAMDVTSEEQVNAAVAEVAAAYGGVDVLVSNAGIQIVHPIEEFP
                     FADWKKMLAIHLDGAFLTTKACVPHMQKSGRGGSIIYMGSVHSKEASVLKSAYVTAKH
                     GLIGLCKTVSKEGGKYGIRANVICPGFVRTPLVEKQIPEQAKALGISEDDVIKKVMLK
                     ETVDGEFTTVQDVAEVALLFAGFESNALTGQSLVVSHGWFMQ"
     gene            complement(2600..3019)
                     /locus_tag="VNU48_13070"
                     /pseudo
     CDS             complement(2600..3019)
                     /locus_tag="VNU48_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase"
     gene            complement(3016..4116)
                     /locus_tag="VNU48_13075"
     CDS             complement(3016..4116)
                     /locus_tag="VNU48_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWI12258.1"
                     /translation="MLQGGGALGAYQAGVLESLSRVYREPTWVAGISIGAINAALITG
                     NPAPARVSKLREFWNLVSSALPAPLIATNPSAREGLNEASAGQVMLFGVPGFFRPRFP
                     PAPFQPRGSLEAISYYDTAPLRETLERLVDFDRINAGAVRLSVGAVNVRTGNFEYFDS
                     SKQTIDARHIMASGALPPGFAPVEIDGEHYWDGGLVSNTPLQYVLDQPGKRRRVVFQV
                     DLFAATGALPTTLPEVTEREKDIRFSSRTRLNTTNELDRQVIGQAVKRLLAKLPPEWH
                     DDPDLRALSRLRCESAVDVVHLIYRSKHYESQSKDYEFSRLSMQEHWDAGRADMAHTL
                     HDPRWVHRERNASGVHVFDLTSDSPTAAKVVP"
BASE COUNT          613 a         1426 c         1504 g          687 t
ORIGIN      
        1 gcgtcggcgt ggatcaggtg gctgggcgtg gcgacctgcg agcgcgcctg ccgcccttcg
       61 atgtagctgc aagggtaggg cgcggtcgcg tagaactgca gtgaggcgag cgggagttct
      121 ttcgggtacg tcacgggccg ggttcctcgg cggcgggccc cagatgccgc cacaggcgca
      181 aatcataggt ccaatcggtg atcggcggct cccccagact gtgggcgagg cgcgcctcga
      241 actcgggccg gcgcaactcg cgcgcaccca tcgactcgag atggcgcgtg tgctgctggc
      301 agtcgatcat cggcaccctg tgctcgcggc agaagcacac cagcgccgcg aacgcgatct
      361 tcgacgcgtc ggtgcggtgt gcgaacatcg attcgccgaa gaacatgtgg cccagaccga
      421 tgccgtacag gccgccgacg agttcgccgt cgacccaggt ctcgaagctg tgcgcccggc
      481 cgagcgcgtg ccagtcgcag taggcctcga tcatctcggg cacgatccag gtgccgtcct
      541 ggccctcgcg cggggtctgc gcgcacgccg tgatgacacg ccggaacgcc gagtcgatgc
      601 gcagctcgca gcgcgtgctc gcggcgaagc gccgcaccgc cttgcgcagc gagcgcgaga
      661 ggcggaactc gtcggtgaac agcaccatgc gcgggtcggg cgaccaccac agcaccggct
      721 ggccttcgct gtaccagggg aacacgcctt tcgcataggc ttcggtcagg cgctgcggcg
      781 tcgggtggcc gcccgcggcg agcaggcccg gcgcatcgct gccgagcggc aaggccgcgc
      841 cggtggccgg cagcggatcg tccgggccgc gcagccagtg gatcacgatg gaaggcctgc
      901 agcgattcgc atgcgccgat tgtcatggct tttgcgccac ggccggcagg tcagaatcgg
      961 tgccttgcct gaccgcgggg attctgatgg actcacgatt tcgatcgatc ggcagtgccg
     1021 ctgcactcgc cgcgctgacg ctgccgaccg gtgcggcgca agccgcggac ggcgacgggt
     1081 tcgcgccgat cacgccgtac cggccgtcgg tgtcgagccc ggcgcagctg cccgcgccgg
     1141 gccagatcga gctcgagttc ggcggcctgc gccagcgctc ggacgacgcg cggcacagca
     1201 gcctgcccta cctgttcaag ctcgcgttcg atgcttcgtg gggcctgctg ctcggcggcg
     1261 aggcgcgggt ctggcagcgc gatggcggcg cccgcgcgca ggggctgggc gacacgagcg
     1321 tcgtgctcaa gcgcgcctgg ctcgtcgacg agggctcggc cttcggcgtc gagctcggcg
     1381 cgaagctgcc gaccgcgaac gacacgatcg gcagcggcca cgcggactat tcgatcaaca
     1441 cgatctacag ccgcgacttc ggcgcgctgc acatggacgc gaacctgaac gcgctgcgct
     1501 tcggccgcgt cgatgccggc acctcgcgca cgcagtgggg cgcgtcggca tcgttctcga
     1561 ccgcgctcgc ggaacactgg ggcgcgaccg gcgaactctc gggaacgcgc cgcaccggcg
     1621 tcgaccacgg cgcgcagctg ctggccgcgc ttgcctacag cccgagcaag ttcctgacga
     1681 tcgacttcgg catcgcgcgc tcgccgcgcc cggcgccggc gacgacctcg ctgttcgccg
     1741 gcgtggtgtt cccgctggcc agactctggt agaggctact gcatgaacca gccgtggctg
     1801 acgaccagcg actggccggt cagcgcgttc gactcgaagc ctgcgaacag cagcgcgacc
     1861 tcggccacgt cctgcacggt cgtgaattcg ccgtccacgg tttccttcag catgaccttc
     1921 ttgatcacgt cgtcctcgct gatgcccagc gccttggcct gctcggggat ctgcttctcg
     1981 accagcggcg tgcgcacgaa gcccgggcag atcacgttgg cgcggatgcc gtacttgccg
     2041 ccttccttcg agaccgtctt gcacaggccg atcagcccgt gcttggcggt gacgtaggcc
     2101 gacttcagca ccgacgcctc cttcgagtgc accgagccca tgtagatgat gctgccgccg
     2161 cggccggact tctgcatgtg cggcacgcag gccttggtcg tcaggaacgc gccgtcgagg
     2221 tggatcgcga gcatcttctt ccagtcggcg aacgggaatt cctcgatcgg gtggacgatc
     2281 tggatgccgg cgttgctgac cagcacgtcg acgccgccat aggccgcggc gacctcggcg
     2341 accgcggcgt tgacctgctc ctcgctggtc acgtccatcg ccaccgccat tgcggtgccg
     2401 ccggccttga cgatctcgtc ggccgcttcc tgtgcggccg ccttgttcat gtcggcgatc
     2461 acgaccttgc cgccttcgcg cgcgtaggtc agggcgattt ccttgccgat gccgctggcg
     2521 gcgccggtga cgatgcagac cttgtcctgg agtttcatga gggtggttcc gtgtgaggtt
     2581 gagagaaagg aaggcggggc tgtaccgagc cgtagtcgag cgtacccggc agcgtgtcgg
     2641 tctcgaccgc gagcttgggc gacgcgagct tcttcgggaa gccccacggc tcgcgcccgc
     2701 cggcgatcgg cgcctcgtcg ttcaggtaca tcgagtgcac atagccgccg gcgcggccct
     2761 cgaacgtgac cgggatcacc tggcccgatt cggtgtagtc gccgaagccg gtcgaatcgg
     2821 gcatgcggat gaactcgtac ttgacgatcg gctcggtgat ctgcagcggc tcgggcacga
     2881 cggcgcgcaa ggcgtcgatg tcggtgcggt agctgacgat caggtgctcg cggttgacga
     2941 agcggtacgg gccgggcggg aaggccgggc tggtcagcgg catcgcgaag gcgctgcgtt
     3001 tcacgtcgtc gatcttcatg gcacgacctt ggcggcggtg ggactgtcgg atgtcaggtc
     3061 gaacacatgc acgccgctgg cgttgcgctc gcggtggacc cagcgcgggt cgtgcagcgt
     3121 gtgcgccatg tcggcgcggc cggcgtccca gtgctcctgc atcgacaggc gcgagaactc
     3181 gtagtccttc gactggctct cgtagtgctt gctgcggtag atcaggtgca ccacgtcgac
     3241 cgcgctctcg cagcgcaacc gcgacagcgc acgcagatcg gggtcgtcgt gccactcggg
     3301 cggcaacttc gccagcagcc gcttgacggc ctgcccgatc acctgccggt cgagttcgtt
     3361 ggtggtgttc agccgcgtgc gactggagaa gcggatgtcc ttctcgcgtt cggtcacctc
     3421 gggcagcgtg gtcggcaagg cgccggtggc ggcgaacagg tcgacctgga acaccacgcg
     3481 ccgccgcttg cccggctggt cgagcacgta ctgcagcggc gtgttcgaga ccagcccgcc
     3541 gtcccagtag tgctcgccgt cgatctcgac cggcgcgaag cccggcggca gcgcgccgct
     3601 ggccatgatg tggcgcgcat cgatggtctg cttgctcgaa tcgaagtact cgaagttgcc
     3661 ggtgcgcacg ttgacagcgc cgaccgacag ccgcaccgcg cccgcgttga tgcggtcgaa
     3721 gtcgaccagc cgctccagcg tttcgcgcag cggcgcggtg tcgtagtagc tgatggcctc
     3781 gagcgagccg cgcggctgga acggtgcggg cggaaagcgc ggccggaaaa agccgggcac
     3841 gccgaacagc atcacctggc ccgcgctcgc ctcgttcagg ccctcgcgtg cactcgggtt
     3901 ggtggcgatc aacggtgccg gcagcgccga gctgaccagg ttccagaact cgcgcagttt
     3961 cgacacccgc gccggcgcgg ggttgccggt gatcagcgcg gcgttgatcg cgccgatcga
     4021 gatgcccgcg acccaggtgg gttcgcggta gaccctgctg agcgactcga gcacgccggc
     4081 ctggtaggcc ccgagcgcgc cgccaccctg gagcacgagg atgcagtctt cgtcagcccg
     4141 atggcgatga tcgtgtgccg cgtcggcagg agcgggcaga acggggcgca gggacatggg
     4201 ctgggtgcgg gaagatcgcc gccaccgcgg
//
LOCUS       DATMJB010000618         3572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_570364, whole genome shotgun sequence.
ACCESSION   DATMJB010000618 DATMJB010000000
VERSION     DATMJB010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570364"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1286
                     /locus_tag="VNU48_13080"
     CDS             231..1286
                     /locus_tag="VNU48_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI12259.1"
                     /translation="MTALNLARRALTGAFAATLLIALTARADEAAKDTPIKFQLDWRF
                     EGPAALFLVPVAKGYFKAEKLNVTVDAGNGSGGAVTRVASGSYDMGFADLAALMEFHA
                     NNPGAANKPVAVMMVYNNTPAAVLALKKSGIKSPADLSGRKMGAPVFDAGRKAWPIFA
                     KANGVTNVAWTAMDPPLRETMLVRGDVDAITGFSFTSLLNIEARGVKADDVVVLPYPS
                     YGVKLYGNAIIVGEEFLKKNPEAVKAFLRAFTKGMRDVIADPKGSIATVKARDGIIDV
                     ALEERRLKLALDATVLTPDARAEGFGMALAPRLSLMASQVSDAFATKERVKPDAVWNG
                     AFLPSAAERNIFAVAKK"
     gene            1283..2107
                     /locus_tag="VNU48_13085"
     CDS             1283..2107
                     /locus_tag="VNU48_13085"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI12260.1"
                     /translation="MSSVGRPFVDFEGVWLAYNDEALAQGQFAVEGISLQAAGGEFIA
                     IVGPSGCGKSTFMKLATGLRMPSKGTIHIDGAPVTGPLKISGMSFQAPSLLPWRSTVD
                     NVLLPLEIVEPHRSQFKARRDEYRERALRLLRTVGLAGYEDKLPHQLSGGMQQRASIC
                     RALIHEPKMLLLDEPFGALDAFTREELWCTLRDLWEAQRFNVILVTHDLRESVFLADT
                     VYVMSRSPGRFVLRREIALPRPRDLELTYTSAFTDLVHELRGHIGAARRATAEAAR"
     gene            2104..2871
                     /locus_tag="VNU48_13090"
     CDS             2104..2871
                     /locus_tag="VNU48_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI12261.1"
                     /translation="MTPKTLQRWAPVVTAAALLLLWQLVCGLFSIADYIFPSPLTIAQ
                     AMREFAAPIAEAAWKTFWVTMLGFAIAIVVGVLLGFLVGSSRLAYAAIYPLLVGFNAV
                     PKAALVPILIVWFGIGLGPGILTAFLISFFPITVNIATGLATLEPELEDVLRVLGAKR
                     WDVLVKVGLPRSMPYFYASLKVAITLAFVGTVLAEMTAGDSGIGYLMQTAGSQQRMPL
                     AFAGLVVIGVMAMTMYELFSLIEKRTTGWAHRGTQGD"
     gene            2927..3394
                     /locus_tag="VNU48_13095"
     CDS             2927..3394
                     /locus_tag="VNU48_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HWI12262.1"
                     /translation="MQRHLRAANAIAQRALAAGHHPFGALLVGPDHETVLVEQGNIDG
                     VNHAEAVLARMAAKAWSADELWHCTLYTTVEPCCMCAGTQYWAHIGRLVYGMAEHRLH
                     QLTGDHPENPTMDLPCREVFARGQKDIRVWGPVPEVEAEIAALHLAFWQRDRA"
     gene            3394..>3572
                     /locus_tag="VNU48_13100"
     CDS             3394..>3572
                     /locus_tag="VNU48_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HWI12263.1"
                     /translation="MAWAGSLRLDYRRDLLREVPRTVLHDRHDGPLRVLASLYPESPA
                     VCHNVLVHPPGGLVGG"
BASE COUNT          528 a         1262 c         1245 g          537 t
ORIGIN      
        1 ctgcgccgcg cctccgagtt gtgaaggacc cagcttctca agtccgggcg ccgcaggctg
       61 tcggacaacg ccgcccgccg gtgaccgact tggcggaccg cgaacgacgg atcagaccga
      121 cttcgggcgg ccgcagcctg cctcgcgctt gtcccgatgg ctccccgggc gcggcgcgca
      181 cagaatttgc gtgtcccgtc gacctgacca cttcctccgg agcccacccg atgaccgcgt
      241 tgaatctcgc ccgccgtgcc ctgaccggcg ccttcgccgc gacgctgctg atcgccctga
      301 cggcccgcgc cgatgaggcg gcgaaggaca cgccgatcaa gttccagctc gactggcgct
      361 tcgagggccc ggccgcgctg ttcctcgtgc cggtcgcgaa aggctacttc aaggccgaga
      421 agctcaacgt cacggtcgat gccggcaacg gctcgggcgg cgcggtcacg cgcgtcgcct
      481 cggggtccta cgacatgggc ttcgccgacc tggccgcgct gatggagttc cacgccaaca
      541 accccggcgc ggcgaacaag ccggtcgcgg tgatgatggt ctacaacaac acgccggcgg
      601 cggtgctggc gctgaagaag tccggcatca agagcccggc cgacctgagc ggcaggaaga
      661 tgggcgcgcc ggtattcgac gccggccgca aggcctggcc gatcttcgcg aaggccaacg
      721 gcgtcaccaa cgtcgcctgg accgcgatgg acccgccgct gcgcgagacg atgctggtgc
      781 gcggcgatgt cgacgcgatc accggcttct cgttcacctc gctgctgaac atcgaggcgc
      841 gcggcgtgaa ggccgacgac gtcgtcgtgc tgccgtaccc gagctacggc gtgaagctgt
      901 acggcaacgc gatcatcgtc ggcgaagagt tcctgaagaa gaatcccgag gccgtgaagg
      961 ccttcctgcg cgcgttcacg aagggcatgc gcgacgtgat cgccgacccg aagggcagca
     1021 tcgccaccgt caaggcgcgc gacggcatca tcgacgtcgc gctcgaagag cgccgcctga
     1081 agctcgcgct cgacgccacg gtgctgacgc ccgacgcgcg cgccgaaggc ttcggcatgg
     1141 cgctggcgcc gcgcctgagc ctgatggcga gccaggtgtc cgacgccttc gccaccaagg
     1201 agcgcgtcaa gcccgacgcg gtctggaacg gcgccttcct gccgagcgcg gccgagcgca
     1261 acatcttcgc ggtcgcgaag aagtgagttc ggtcggccgg cccttcgtcg acttcgaggg
     1321 ggtctggctc gcctacaacg acgaggcgct ggcgcagggc cagttcgcgg tcgaagggat
     1381 ctcgctgcag gcggccgggg gcgagttcat cgcgatcgtc gggccgtcgg gctgcggcaa
     1441 gtcgaccttc atgaagctcg ccaccgggct gcgcatgccg agcaagggga cgatccacat
     1501 cgacggcgcg ccggtcaccg gcccgctgaa gatcagcggc atgtcgttcc aggcaccgtc
     1561 gctgctgccg tggcgcagca cggtcgacaa cgtgctgctg ccgctggaga tcgtcgagcc
     1621 gcatcgctcg cagttcaagg ccaggcgcga cgagtaccgc gaacgcgcgc tgcgcctgct
     1681 gcgcaccgtg ggcctggccg gctacgagga caagctgccg caccagctct cgggcggcat
     1741 gcagcagcgc gcgtcgatct gccgcgcgct gatccacgag ccgaagatgc tgctgctcga
     1801 cgagcccttc ggcgcgctcg acgcgttcac gcgcgaggag ctgtggtgca cgctgcgcga
     1861 cctgtgggaa gcgcagcgct tcaacgtcat cctggtcacg cacgacctgc gcgagagcgt
     1921 gttcctggcc gacaccgtct acgtgatgag ccgcagcccc gggcgcttcg tgctgcggcg
     1981 cgagatcgcg ctgccgcggc cgcgcgacct ggagctgacc tacacgagcg cgttcaccga
     2041 cctcgtccac gagctgcgcg gccacatcgg cgcggcgcga agggcgacgg ccgaggcggc
     2101 gcgatgacgc cgaagacatt gcagcgctgg gcgccggtcg tcaccgcggc ggcgctgctg
     2161 ctgctgtggc agctcgtgtg cgggctgttc tcgatcgccg actacatctt cccgagcccg
     2221 ctgacgatcg cgcaggcgat gcgcgagttc gccgcgccga tcgccgaggc cgcgtggaag
     2281 accttctggg tcacgatgct gggcttcgcg atcgcgatcg tcgtcggcgt gctgctcggg
     2341 ttcctggtcg gttcgtcgcg gctggcgtat gcggcgatct acccgctgct ggtgggcttc
     2401 aacgcggtgc cgaaggcggc gctggtgccg atcctgatcg tctggttcgg catcggcctg
     2461 gggcccggca tcctgaccgc gttcctgatc tcgttcttcc cgatcacggt caacatcgcc
     2521 accggcctgg ccacgctcga gcccgagctc gaggacgtgc tgcgcgtgct cggcgcgaag
     2581 cgctgggacg tgctcgtcaa ggtcggcctg ccgcgctcga tgccctactt ctacgcctcg
     2641 ctgaaggtcg cgatcacgct cgccttcgtc ggcacggtgc tggccgagat gacggccggc
     2701 gactccggca tcggctacct gatgcagact gcgggttcgc agcagcgcat gccgctggcc
     2761 ttcgccggcc tggtggtgat cggcgtgatg gcgatgacga tgtacgagtt gttctcgctg
     2821 atcgagaagc gcaccaccgg gtgggcacat cggggaaccc agggtgactg aaacctacga
     2881 cgggccatgg ccggcgccac cgggcgacga gccgacgccg cagcagatgc agcgccactt
     2941 gcgcgccgcc aacgcgatcg cgcagcgcgc gctcgcggcc ggccaccacc cgttcggcgc
     3001 actgctggtc gggcccgatc acgagaccgt gctcgtcgag cagggcaaca tcgacggcgt
     3061 caaccatgcc gaggcggtgc tcgcgcgcat ggccgcaaag gcctggtccg ccgacgagct
     3121 ctggcactgc acgctgtaca ccacggtcga gccctgctgc atgtgcgccg gcacgcagta
     3181 ctgggcccac atcggccggc tcgtctacgg catggccgag caccggctgc accagctcac
     3241 cggcgaccac cccgagaacc cgacgatgga tctgccctgc cgcgaggtct tcgcgcgcgg
     3301 gcagaaggac attcgcgtct ggggcccggt gcccgaggtc gaggccgaga tcgccgcgct
     3361 gcacctggcg ttctggcagc gcgaccgcgc ctgatggcct gggccggctc gctgcggctc
     3421 gactaccggc gcgacctgct gcgcgaggtg ccacgcacgg tgctgcacga tcgccacgac
     3481 ggcccgctgc gcgtgctcgc gagcctgtac cccgagagcc ccgcggtctg ccacaacgtg
     3541 ctggtgcacc cgcccggcgg gctggtcggt gg
//
LOCUS       DATMJB010000619         3095 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_865846, whole genome shotgun sequence.
ACCESSION   DATMJB010000619 DATMJB010000000
VERSION     DATMJB010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3095
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865846"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /locus_tag="VNU48_13105"
     CDS             complement(<1..1608)
                     /locus_tag="VNU48_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid ferredoxin oxidoreductase"
                     /protein_id="HWI12264.1"
                     /translation="MASRQDTPNIFIQEEGVIHLGGIDALVRLTLDQVRTDARRGLKT
                     GMFVSGYRGSPLGMLDAALLKQRKLLLEHHINFVDGLNEDLAATAVWGTQMLHTVGQQ
                     KFDGVTGLWYGKAPGVDRSGDALKHANYTGIGKNGGVLAVAGDDPSCKSSSLSSQSEP
                     MLMHVGIPSLYPGNVQEILDFGLHGYNMSRLSGLWVGLKIVTNVADGSGSADVTPERL
                     NFITPDMMFDGKAFAPRMNLGMNVRAEALEMEESLYTRRLEVAKRYARANKLNHVVFE
                     NPDAWLGIITAGKTWNDLRQCFLELGLDDAALRRHGIRILKMGMLFPMEPTVVREFAR
                     GLEEIFVIEEKRPFLEMFAKNVLYGQANAPRIVGKFDEEEKELLPHYGEFESDVIGRA
                     LTQRLSKKARVESAEAWLARLDEIHARTKVPTATRTAWYCSGCPHSSSTQAVEGSIVS
                     AGIGCHTMAMWMGRNVVMGTHMGAEGAQWIGMAPFTDVKHIFQNMGDGTYAHSGSLAI
                     RYAASTHTNITFKVLVNAHTSMTGGQEI"
     gene            complement(1733..>3095)
                     /locus_tag="VNU48_13110"
     CDS             complement(1733..>3095)
                     /locus_tag="VNU48_13110"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWI12265.1"
                     /translation="CRVDLGSHFYSYSFEPSHEWSQYFAQRDELHAYFNRFADKHGLR
                     EHIRFQTEVVSADWDEAAQRWRVALRDAAGKTRHVDASAMVSAVGQLNRPMIPVFEGQ
                     DQFKGLQVHSAQWRSDIDLAGKRVAVIGTGATAVQLVPELAKVASGLTVFQRSPIWLL
                     PNPRYHLPVSEEEKWLLQHLPFYARWYRFLLFWPGTDGILPNLRIDPDWAHPDRSINA
                     HNEEMRQQMVAYIESQVGDDPELLKKVVPPYPVMVKRMNQDNGSWFRALKQPHVDLQT
                     GRIQRIDATGIWCDGVHHEVDAIVYCTGFQASKFLWPMQITGRNGLRLDEFWGDEPGA
                     YLGVTVTGFPNLYCMYGPATNLAHGGSLIQHSEWQGRYIAGCIKATIDSGKRAMEVKP
                     EVYAAFNKKLVETLEQMVWSHGGTTSWYRNKNGRVVTTSPWRLVDYRDWLHAPKLEEY
                     RLS"
BASE COUNT          480 a          991 c         1049 g          575 t
ORIGIN      
        1 gatctcctgc ccgcccgtca tcgaggtgtg cgcgttgacg agcaccttga aggtgatgtt
       61 ggtgtgggtc gatgcggcat agcggatcgc caggctcccg gagtgggcgt aggtgccgtc
      121 gcccatgttc tggaagatgt gcttgacgtc ggtgaacggc gccatgccga tccactgcgc
      181 gccttcggcg cccatgtgcg tgcccatcac cacgttgcgt cccatccaca tggccatcgt
      241 gtggcagccg atgccggccg agacgatgct gccctcgacc gcctgggtcg agctgctgtg
      301 cgggcaaccc gagcagtacc aggcggtgcg cgtcgcggtg ggcactttgg tgcgggcatg
      361 gatctcgtcg agtcgggcca gccaggcctc ggccgattcg acgcgcgcct tcttcgacag
      421 ccgctgggtc aacgcgcgac cgatcacgtc ggactcgaac tctccgtagt gcggcaggag
      481 ttccttttcc tcctcgtcga acttgccgac gatgcgcggg gcgttggctt gcccgtagag
      541 aacgttcttg gcgaacatct cgaggaaggg gcgcttctcc tcgatcacga agatctcttc
      601 caggccgcgg gcgaattcgc gcacgaccgt cggctccatc gggaacagca tgcccatctt
      661 caggatgcgg atgccgtggc ggcgcagcgc ggcgtcgtcc agaccgagct cgagaaagca
      721 ctggcgcagg tcgttccagg tcttgcccgc ggtgatgatg ccgagccacg cgtcggggtt
      781 ctcgaacacg acatggttga gcttgttcgc gcgcgcgtag cgcttggcga cctcgagacg
      841 gcgggtgtag agcgactcct ccatctcgag cgcttcggcg cgaacgttca tgccgagatt
      901 catgcgcggc gcaaacgcct tgccgtcgaa catcatgtcg ggcgtgatga agttcagccg
      961 ctccggcgtg acgtcggccg agcccgaccc gtcggcgacg ttggtgacga tcttcaggcc
     1021 gacccacaga cccgacaggc gcgacatgtt gtagccgtgc agcccgaaat cgaggatctc
     1081 ttgcacgttg ccgggataga gcgacggaat ccccacgtgc atcaacatcg gttcggactg
     1141 gctgctcagc gaggaactct tgcaactcgg gtcgtcaccg gccaccgcga gcacgccgcc
     1201 gttcttgccg atgccggtgt agttggcgtg cttcaaggcg tcgccgctgc ggtccacgcc
     1261 gggcgccttg ccgtaccaca agccggtcac gccgtcgaac ttctgttggc ccacggtgtg
     1321 cagcatctgc gtgccccaca ccgcggtggc ggcgaggtct tcgttgagac cgtcgacgaa
     1381 gttgatgtgg tgctcgagca gcagcttgcg ctgcttgagc agtgcggcgt cgagcatgcc
     1441 cagcggcgat ccccggtagc ccgagacgaa catgccggtc ttcaggccgc ggcgcgcgtc
     1501 ggtgcgcacc tggtccagcg tcaggcgcac cagcgcgtcg atgccgccga ggtggatgac
     1561 gccctcttcc tgaatgaaga tgttgggtgt gtcttgtctg gatgccatgg ggtgtcctct
     1621 gtgctgcgca ggcatcgtca ccaggacgcc gcgctggcgc ggttcgggcg caagccgggg
     1681 ccatctggaa agaggggaaa gccgtgcact cgcacggctc tgccggggtc gatcaactca
     1741 gccggtattc ctcgagcttg ggtgcatgca gccagtcacg gtagtccacc aggcgccacg
     1801 gcgacgtggt gacgacccgg ccgttcttgt tgcgatacca ggaggtggtg ccgccgtgcg
     1861 accagaccat ttgctcgagc gtctcgacga gcttcttgtt gaaggcggcg tagacctcgg
     1921 gcttgacttc catcgcgcgc ttgccgctgt cgatggtggc cttgatgcag ccggcgatgt
     1981 agcgcccctg ccattcgctg tgctggatga gcgagccgcc gtgcgccagg ttggtggccg
     2041 ggccgtacat gcagtacagg ttcggaaagc cggtgacggt gacgcccaga taggcgcccg
     2101 gctcgtcgcc ccagaactca tcgagccgca ggccgttgcg gccggtgatc tgcatcggcc
     2161 acaggaactt gctggcctgg aagccggtgc agtagacgat cgcatccacc tcgtggtgca
     2221 ccccgtcgca ccagatgccg gtggcatcga tgcgctggat gcggccggtc tgcagatcga
     2281 cgtgcggctg cttcaaggcg cggaaccagc tgccgttgtc ctggttcatg cgcttgacca
     2341 tcaccgggta cggcggcacc accttcttca gcaactcggg gtcgtcgccc acctgcgatt
     2401 cgatgtaggc caccatctgc tggcgcatct cctcgttgtg cgcgttgatg gagcgatcgg
     2461 ggtgagccca gtcggggtcg atcctgaggt tcggcaggat gccgtcggtc cccggccaga
     2521 acagcaggaa gcggtaccag cgcgcgtaga acggcaggtg ctggagcagc cacttctctt
     2581 cctcgctcac aggcaggtga tagcgcgggt tgggcagcag ccagatcggc gagcgctgga
     2641 acaccgtcag cccactggcc accttggcca gctcgggcac cagctgcacc gccgtggcac
     2701 cggtgccgat gacggccacg cgcttgcccg cgaggtcgat gtcggatcgc cattgcgccg
     2761 agtgcacctg caggcccttg aactggtcct ggccctcgaa cacgggaatc atcggccgat
     2821 tgagctggcc gaccgcgctg accatggcgc tggcatcgac gtgccgtgtc ttgccggccg
     2881 cgtcgcgcag ggccacgcgc caccgttgcg cggcctcgtc ccagtcggca ctcaccactt
     2941 cggtctggaa ccggatgtgc tcgcgcaggc cgtgcttgtc ggcgaagcgg ttgaagtagg
     3001 cgtgcagctc gtcgcgctgc gcaaagtact gactccactc gtggctgggc tcgaacgagt
     3061 aggagtagaa gtggctgccc aggtcgacgc ggcag
//
LOCUS       DATMJB010000620         5017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_381681, whole genome shotgun sequence.
ACCESSION   DATMJB010000620 DATMJB010000000
VERSION     DATMJB010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381681"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..483
                     /locus_tag="VNU48_13115"
     CDS             187..483
                     /locus_tag="VNU48_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12266.1"
                     /translation="MKSTLEVEANGFARFVALMGEMVPILEREGWKLESAFMHTSGRL
                     NTVVDVWRLEDYNHYGRGLEVLMAHPEFPRFGQILGEVVRNETIVFLQALEYPR"
     gene            534..1250
                     /locus_tag="VNU48_13120"
     CDS             534..1250
                     /locus_tag="VNU48_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014899307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI12267.1"
                     /translation="MKRMQDKVVLVTGAASGLGAADAALLAHEGATVIVTDIAEAAGR
                     AVAERVGGEFMRHDVRLEADWARVMGSVLARHGRLDVLVNNAGICRVGDIETTTLEQY
                     QLLAAVHGEATFLGCRHAVQVMKERGGSIINISSVAALRATPPVLAYAAAKGAIRALS
                     LSVAGHCRDKGYPVRCNTIFPGVIDTPLVVPVVGANFPGAGQPDDVANMVLFLASDES
                     GFVTGAEFVVDNGATARLAA"
     gene            1273..2055
                     /locus_tag="VNU48_13125"
     CDS             1273..2055
                     /locus_tag="VNU48_13125"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI12268.1"
                     /translation="MPRLQDKVAIVLGAAGRDNMGQEIARRFAAEGARVVVAGRHAEE
                     LKRFAATIGGSAKVCDITRKADLQALAAHAKAWGGHLDIAVNATGWGLLKPFLEITED
                     EIDRITALQFKGPHLFFQAVLEVMAAGGSIIQISSATATIMLGDHAAYMGTKAGTDHV
                     VRCIANEFGHRGIHANSLSPGLTDTPMTAQAKAVPGLFEAFLPGYPLGRIGTKADIAA
                     AAVWLASDECFMTGENLQVNGGLTLRRNPSGAEIAASVAAHA"
     gene            2066..2608
                     /locus_tag="VNU48_13130"
     CDS             2066..2608
                     /locus_tag="VNU48_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWI12269.1"
                     /translation="MASRKSLIAQLGTVMQMAYVPQDFDAALRYWTETMGVGPFFSLP
                     HISLPNCRYEGRPSDIDFSIAIAYWGDIQIELVRQHNDAPSIYKRWRDEGRDGLHHVC
                     IVVDDMAHARSVCAAAGARIAQEADVAGGGEVIYVDAGGGPGGLIELIQLPQTTLAGF
                     AAMREVCRHWDGSEPLRPLG"
     gene            2622..3410
                     /locus_tag="VNU48_13135"
     CDS             2622..3410
                     /locus_tag="VNU48_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HWI12270.1"
                     /translation="MNPNDPMPTRIDVVLIASGKYHDIDFARLELLKLLAEDECLRVR
                     VFEDYAHLDAIRRARFIVTYTCDVVPSLEQQEALRAWVEAGGRWLALHGTNSILRFLA
                     NGLVDSPAWAPHLMQTLGTQFIAHPAIAPYRVEVADPQHALVRGIEPFETTDELYLVD
                     AHPGLQVLLDTEFEGTAEGFVRERWPRARHPVMYLRELGQGAVLYLTLGHCRGHYDLQ
                     PLMDYWPSVDRCAWDLPVFHELLRRGLAWAGTRDSHPHPPTTPA"
     gene            3424..4182
                     /locus_tag="VNU48_13140"
     CDS             3424..4182
                     /locus_tag="VNU48_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI12271.1"
                     /translation="MELGLHGKKVIINGGSRGIGRAALDIYAAEGCDIAFFSRDAKRV
                     DETVTQLSKAGVKVFGDALDMNDSAVYEAWLKSAVQRLGGCDIFIHNASSSGAQATTD
                     WDKTFQLDMMGAVTGCQTLEPALEASGSGSVILMSSTAAVETFIYPQAFNAIKAAILT
                     YGKQLSQLWAAKKIRVNMVCPGPIAYPGGNWSMIKDGMPELYAKTLSEMPLGRFGAPE
                     DVARSMVFLSSPMASYITGSNLVIDGGYTKRVQF"
     gene            4194..4811
                     /locus_tag="VNU48_13145"
     CDS             4194..4811
                     /locus_tag="VNU48_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HWI12272.1"
                     /translation="MKLYQSVGPNPRLVRMFLIEKGIELPTVDVDIIAGENRQPPFLA
                     INPSGGTPVLMLDSGQALAETTAICEYLEELHPQPPLIGSTPEARARTRMWTRRIDLA
                     VAQPMTSGYRAAEGMPLFKDRVRCLPQAADDLKVTAREGLAWLEAQLGERPFIAGDTP
                     TLADIVLYSFVEFGALVGQGLDPGHARLVAWHRRMAERDSAKATA"
     gene            4817..>5017
                     /locus_tag="VNU48_13150"
     CDS             4817..>5017
                     /locus_tag="VNU48_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013956755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin"
                     /protein_id="HWI12273.1"
                     /translation="MSTELAWSDALLLGHGPMDRTHREFVEVVQAMMRCADVGLATGL
                     RDFIAHAQAHFGDEDRSMGETAF"
BASE COUNT          826 a         1749 c         1687 g          755 t
ORIGIN      
        1 tttccacgcc agcctatcca cctggagcta cggcatgaaa caccttaggt caacttgcgc
       61 gcggcgggcc gagcgccggg caagggccac gcaggggccc ctgcgtggcc cagccaagcc
      121 ggcccgcatc cccttggggg atcggccgac gtacccgtcg gacgaggggc agacatgacc
      181 cttcatatga aatcgacgct ggaggtcgag gccaacggct tcgcccgctt cgtcgcgctg
      241 atgggcgaga tggtgcccat cctcgaacgc gagggctgga agctcgagag tgccttcatg
      301 cacaccagcg gccggctgaa cacggtggtc gatgtgtggc ggctggagga ctacaaccac
      361 tacggccgcg ggctcgaggt gctgatggcc cacccggagt ttccgcgctt cggccagatc
      421 ctgggcgagg tggtgcgcaa cgagaccatc gtgttcctgc aggccctgga atatccgcga
      481 taaaaacaaa cggacgagat cgtttgtatt ggaatacgcg aaaggaattg tcgatgaagc
      541 gcatgcagga caaggtggtg ctggtcaccg gcgcggcctc cgggctgggc gcggcggacg
      601 ccgcactgct ggcgcacgaa ggcgcgacgg tgatcgtgac cgacatcgcc gaagcggccg
      661 gccgcgcggt cgccgaacgc gtgggcggcg agttcatgcg ccacgacgtg cggctggagg
      721 ccgactgggc gcgcgtgatg ggcagcgtgc tggcgcgcca cggcaggctc gacgtgctgg
      781 tcaacaacgc cggcatctgc cgcgtcggcg acatcgagac caccacgctc gaacagtacc
      841 agctgctcgc cgcggtgcat ggcgaggcca ccttcctcgg ctgccggcac gcggtgcagg
      901 tgatgaagga gcgcggcggc tcgatcatca acatctcctc ggtcgccgcg ctgcgggcca
      961 cgccgccggt gctggcctat gccgcggcca agggcgcgat ccgcgcgttg agcctgtcgg
     1021 tcgccggcca ctgccgcgac aagggctacc ccgtgcgctg caacacgatc ttccccggcg
     1081 tcatcgacac gccgctggtg gtgccggtgg tcggcgcgaa cttccccggc gccggccagc
     1141 ccgacgacgt ggcgaacatg gtgctgttcc tcgcgtccga cgagtcgggt ttcgtcaccg
     1201 gtgccgagtt cgtcgtcgac aacggcgcca ccgcgcgcct ggccgcttga acctgggttg
     1261 aaaggtccac cgatgccacg cctgcaagac aaggtcgcga tcgtgctggg ggcggccggc
     1321 cgcgacaaca tgggccagga gattgcgcgc cgctttgccg ccgaaggcgc gcgcgtggtc
     1381 gttgccgggc gtcatgcgga ggagctcaaa cgcttcgcgg ccacgatcgg cggcagcgca
     1441 aaggtgtgcg atatcacgcg caaggccgat ctgcaggccc tggccgcgca tgccaaggct
     1501 tggggcggcc acctcgacat cgcagtcaac gccaccggct ggggcttgct gaagcccttc
     1561 ctggagatca ccgaagacga gatcgaccgc atcaccgcgc tgcagttcaa gggcccgcac
     1621 ttgttcttcc aggcggtgct ggaggtgatg gccgctggtg gctcgatcat ccagatcagc
     1681 tcggccacgg cgacgatcat gctgggcgat cacgccgcct acatgggcac caaggccggc
     1741 accgaccatg tcgtgcgctg catcgccaac gagttcggcc accgcggcat ccatgccaat
     1801 tcgctctcgc ccggcctgac cgacacgccg atgacggcgc aggccaaggc cgtgcccggc
     1861 ctgttcgagg cgttcctgcc gggttatccg ctggggcgca tcggcaccaa ggccgacatc
     1921 gccgccgcgg ccgtctggct ggccagcgac gagtgcttca tgaccggcga gaacctgcag
     1981 gtcaacggcg gcctgacctt gcgccgcaac ccgtccggcg ccgagatcgc ggcctcggtc
     2041 gcagcccacg cctgaaagcc gcaccatggc ctcacgcaaa tccttgatcg cccagctcgg
     2101 caccgtcatg cagatggcct atgtgccgca ggacttcgac gccgcactgc gctactggac
     2161 cgagaccatg ggtgtcggcc cgttcttcag cctgccgcac atctcgctgc cgaactgccg
     2221 ctacgaaggt cggccctcgg acatcgactt ctcgatcgcc atcgcctact ggggcgacat
     2281 ccagatcgag ctggtccggc agcacaacga cgcgccgtcg atctacaagc gctggcgcga
     2341 cgaaggccgc gacgggctgc accacgtgtg catcgtggtc gacgacatgg cacatgcgcg
     2401 cagcgtctgc gccgccgctg gcgcgcgcat cgcccaggaa gccgacgtgg ccggtggcgg
     2461 cgaggtgatc tacgtcgacg ccggcggcgg gccgggcggg ctgatcgagc tgatccagtt
     2521 gccgcagacc acgctggccg gctttgccgc gatgcgcgag gtgtgccgcc attgggacgg
     2581 cagcgagccg ctgcgcccgc tgggttgaac aggacactgc catgaacccg aacgacccca
     2641 tgccgacgcg catcgacgtc gtgctgattg cctccggcaa gtaccacgac atcgacttcg
     2701 cgcggctcga actgctcaag ctgctggcgg aggacgagtg cctgcgcgtg cgcgtgttcg
     2761 aggactacgc ccacctcgac gcgatccgcc gggcccgctt catcgtcacc tacacctgcg
     2821 acgtggtgcc cagcctggag cagcaggagg cgctgcgcgc ctgggtcgaa gcgggagggc
     2881 gctggctggc gctgcacggc accaactcga tcctgcgctt cctggccaat ggcctggtcg
     2941 attcgccggc ctgggcgccg cacctgatgc agacgctggg cacgcagttc atcgcccatc
     3001 cggcgatcgc gccttaccgc gtcgaggtgg ccgacccgca gcatgcgctg gtgcgcggca
     3061 tcgagccctt cgagaccacc gacgagctgt acctcgtcga cgcccacccg gggctgcagg
     3121 tgctgctgga caccgagttc gaaggcacgg ccgaaggctt cgtgcgcgaa cgctggccgc
     3181 gggcgcgaca cccggtgatg tacctgcgcg agctggggca gggggccgtg ctctacctga
     3241 cgctgggcca ctgccgcggc cattacgacc tccagccgct gatggactac tggcccagcg
     3301 tggaccgatg cgcctgggac ctgccggtgt tccacgaact gctgcggcgc ggcctggcct
     3361 gggccggcac gcgcgacagc caccctcacc ccccaaccac ccccgcctga caaggagcgc
     3421 accatggaac tcggactgca cggcaagaag gtcatcatca acggcggcag ccgcggcatc
     3481 ggccgcgcgg cgctcgacat ctacgccgcc gaaggctgcg acatcgcctt cttctcgcgt
     3541 gacgccaagc gcgtcgacga gaccgtcacg cagctgtcga aagccggcgt caaggtcttc
     3601 ggcgatgcgc tggacatgaa cgacagcgcc gtctacgagg cctggctgaa gagcgcggtg
     3661 cagcgcctgg gcggctgcga catcttcatc cacaacgcca gctcgtcggg cgcgcaggcc
     3721 acgaccgact gggacaagac cttccagctc gacatgatgg gcgcggtgac cggctgccag
     3781 accctggagc cggcgctcga ggcttcgggc agcggctcgg tgatcctgat gtcgagcacc
     3841 gcggcggtgg agaccttcat ctacccgcag gccttcaacg ccatcaaggc tgccatcctg
     3901 acctacggca agcaactctc gcagctctgg gcggccaaga agatccgcgt caacatggtc
     3961 tgcccggggc cgatcgccta ccccggcggc aactggtcga tgatcaagga tggcatgccc
     4021 gagctctacg ccaagacgct gtcggagatg ccgctcggcc gcttcggggc ccccgaggac
     4081 gtggcgcggt ccatggtgtt cctgtccagc ccgatggcct cgtacatcac cggcagcaac
     4141 ctggtgatcg acggcggcta caccaagcgc gtgcagttct gacgctggcg accatgaagc
     4201 tctaccaatc cgtcggtccc aatccgcgcc tggtgcgcat gttcctgatc gagaagggca
     4261 tcgagctgcc cacggtcgac gtcgacatca tcgccggcga gaaccgccag cccccgttcc
     4321 tggcgatcaa cccatccggc ggcacgccgg tgctgatgct cgattcgggc caggccctgg
     4381 ccgaaaccac cgcgatctgc gaatacctgg aggaactgca cccgcagccg ccgctgatcg
     4441 gcagcacgcc cgaggcacgc gcccgcacgc gcatgtggac gcgccgcatc gacctggccg
     4501 tcgcgcagcc gatgacgagc ggctatcgcg ccgccgaggg catgccgctg ttcaaggacc
     4561 gcgtgcgctg cctgccgcag gccgcggacg acctgaaggt gacggcccgc gaaggcctgg
     4621 cctggctcga agcccagctc ggcgagcgcc ccttcatcgc cggcgacacg ccgaccctgg
     4681 ccgacatcgt gctctatagc ttcgtcgagt tcggcgcgct ggtcgggcag ggtctcgacc
     4741 ccggccacgc ccggctcgtc gcctggcatc gccgcatggc cgagcgcgac agcgccaagg
     4801 ccaccgcttg aatgcgatga gcaccgaact ggcctggagc gatgcgctgc tgctcggtca
     4861 tgggccgatg gaccgcacgc accgcgagtt cgtcgaggtg gtgcaagcga tgatgcgttg
     4921 tgccgatgtc ggactggcca ccgggttgcg cgacttcatc gctcacgcgc aggcgcattt
     4981 cggcgacgag gatcgatcga tgggcgagac ggcattc
//
LOCUS       DATMJB010000621         3787 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_8554, whole genome shotgun sequence.
ACCESSION   DATMJB010000621 DATMJB010000000
VERSION     DATMJB010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3787
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8554"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..886
                     /locus_tag="VNU48_13155"
     CDS             170..886
                     /locus_tag="VNU48_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI12274.1"
                     /translation="MPAHILVAEDQTDIRELLVMNLRGAGYEVCAVGDGAQALARQLE
                     SPADLLVLDLMMPRMDGLEVCKALRAKGSAAPILMLTAKSTELDRVLGLELGADDYLT
                     KPFSLAELLARVKALLRRADLLAAAQANAQAPGRAAVIRNGELEILPAKRQVRCRGAT
                     IEFTALEFDLLLHFASHPGHVFSRAQLLNAVWGYTHDGYEHTVTTHINRLRAKLEADP
                     MRPELILTARGAGYKMRDLA"
     gene            883..2451
                     /locus_tag="VNU48_13160"
     CDS             883..2451
                     /locus_tag="VNU48_13160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWI12275.1"
                     /translation="MTVRFKLALTIFATGIVTALLVIVTVIYAFQRFERETAYRRGSA
                     FLQRVVGSHDNLFELYARRPDEFQAWLSNLVLFEPDSELYLLDPGGVVLACSGTPLPA
                     GVRVALTPVLRAVGQPDAAYVMGDDPKRMDADAIVAVQPVRRTVIGPADPVAGYLYLV
                     LHNAQSPEGRWATVRMSIARPALGLIVAVVAFTTLLAVLIIASVTRPLQRLTDAVASI
                     SQRGLDAGQPSAPAAALPPVTRDEFGQLTRAFEMMLATLRRQWGELRRLDHFRREGVS
                     NLSHDLRSPLTATVACLETLEGRWPPDGGADPARAEDRRLVEIALRNTRNAARLVQSL
                     GDLSKLDEPEFKLHPEVVDLGELLDDIALRFAERAAQQGVRLRAEADDDAVAPAYAAV
                     DVELFERAIANLVDNALKFCPRGARVTLAARAHAGEVRVSVADTGPGIAPADLPHLFD
                     RFYQSRQSVAPATGEGGKGLGLAIVKRIAELHGGAVAVSSSLGAGTQVVLSLPAPGGA
                     ARLGADRDVGPGNP"
     gene            2756..3586
                     /locus_tag="VNU48_13165"
     CDS             2756..3586
                     /locus_tag="VNU48_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWI12276.1"
                     /translation="MPVDPKLRSLNIDVPAQPESLVKLSLLLADDNVNLQSVSALISG
                     DMALASAVLKAVNSSMYGLRGRVQSVQQAITHLGTREVAAVTFAMGLRAVFPHAPELE
                     PVWERAGVRALLMSRIGQQVGVDPWIAHSAGLFEECGKAVLFRHATERYRALLAKAAD
                     DEELLMLEHTEFGVSHDALGAALCESWGLAAAAVTSVRFHVIVNSTRELPMQVPKRTI
                     CALSALAYALMTDPAALDEVAHAIAPQAGLDPTLVLRGARKVQEQIEAAVARSAAAAG
                     "
BASE COUNT          540 a         1364 c         1351 g          532 t
ORIGIN      
        1 aagtgcgcgg cggcaagggt gggggcgagg ttcatggcgg tccggtgagg cgttggcgat
       61 gcctgaactt tggtcgccgc gcctcgcgcc agcctcaccg aagatcacgg caagatcaca
      121 atcgcgtcaa agcgccgtaa gactcgggcg ctctgcccga cccctctgca tgcctgcgca
      181 catcctcgtt gccgaagacc agaccgacat ccgcgagttg ctggtgatga acctgcgcgg
      241 cgccggctac gaagtctgcg ccgtcggcga cggtgcgcag gcgctggcgc gccagctcga
      301 atcgcccgcc gacctgctgg tgctcgacct gatgatgccg cgcatggacg gcctggaagt
      361 ctgcaaggcg ctgcgcgcca agggcagcgc ggcgccgatc ctgatgctca ccgccaagtc
      421 gaccgagctc gaccgcgtgc tcgggctgga actcggcgcc gacgactacc tgaccaagcc
      481 gttctcgctc gccgagctgc tggcgcgcgt caaggcgctg ctgcggcgcg ccgacctgct
      541 ggccgcagcg caggcgaatg cacaggcgcc ggggcgcgcg gcggtgatcc gcaacggcga
      601 gctggaaatc ctgccggcca agcgccaggt gcgctgccgc ggcgcgacga tcgagttcac
      661 cgcgctcgag ttcgacctgc tgctgcactt cgcgtcgcac cccggccacg tgttctcgcg
      721 cgcgcagctg ctcaacgcgg tctggggcta cacccacgac ggctacgagc acaccgtgac
      781 gacgcacatc aaccggctgc gcgccaagct cgaggccgac ccgatgcggc ccgagctgat
      841 cctcacggcc cgaggtgccg gctacaagat gcgcgacctg gcatgaccgt tcgattcaag
      901 ctcgcgttga cgatcttcgc caccggcatc gtcaccgcgc tgctggtgat cgtgacggtg
      961 atctacgcgt tccagcgctt cgagcgcgag accgcgtacc gccgcggcag cgcgttcctg
     1021 cagcgcgtgg tcggcagcca cgacaacctg ttcgagctgt acgcgcgccg gcccgacgag
     1081 ttccaggcct ggctcagcaa cctggtgctg ttcgagcccg acagcgagct ctacctgctc
     1141 gaccccggcg gcgtcgtgct cgcgtgcagc ggcacgccgc tgccggccgg cgtcagggtc
     1201 gcgctgacgc cggtgctgcg ggcggtcggg cagcccgacg ccgcctacgt gatgggcgac
     1261 gatccgaagc gcatggacgc cgatgccatc gtcgccgtgc agccggtgcg ccgcactgtg
     1321 atcgggcccg ccgacccggt cgccggctac ctgtacctgg tgctgcacaa cgcgcagtcg
     1381 cccgagggcc gctgggccac ggtgcgcatg agcatcgcgc ggccggcgct cgggctgatc
     1441 gtcgccgtgg tcgcgttcac gacgctgctg gcggtgctga tcatcgcctc ggtgacgcgc
     1501 ccgctgcagc gcctgaccga tgcggtcgcg tcgatctcgc agcgcggcct cgatgccggc
     1561 cagccgagcg cgccggccgc ggcgctgccg ccggtcacgc gcgacgagtt cggccagctg
     1621 acacgcgcgt tcgagatgat gctggcgacc ttgcgcaggc aatggggcga gctgcgccgg
     1681 ctcgaccatt tccgccgcga gggcgtcagc aacctgtcgc acgacctgcg ctcgccgctg
     1741 accgcgaccg tggcctgcct cgaaacgctg gagggtcgct ggccgccgga cggcggcgcc
     1801 gacccggcgc gcgccgagga ccggcggctg gtcgagatcg cgttgcgcaa cacgcgcaac
     1861 gcggcgcgcc tggtgcaatc gctgggcgac ctgtccaagc tcgacgaacc cgagttcaag
     1921 ctgcaccccg aggtcgtcga cctcggcgag ctgctcgacg acatcgcgct gcgcttcgcc
     1981 gagcgtgccg cccagcaggg cgtgaggctg cgcgccgagg ccgacgacga tgctgtggcg
     2041 cctgcgtatg cggcggtcga cgtcgagctg ttcgagcgcg cgatcgcgaa cctggtcgac
     2101 aacgcgctga agttctgccc gcgcggcgcc cgcgtcacgc tcgcggcgcg cgcgcacgcg
     2161 ggcgaggtgc gcgtcagcgt cgccgacacc gggcccggca tcgcgccggc cgacctgccg
     2221 cacctgttcg accgcttcta tcagagtcgc cagagcgtcg ccccggccac cggcgagggc
     2281 ggcaagggcc tgggtctggc gatcgtcaaa cgcatcgccg aactccatgg tggtgcggtg
     2341 gccgtcagca gcagcctcgg cgctggaacg caagtggtgc tgtcgctgcc cgcgcctggc
     2401 ggggccgcgc gtctcggcgc cgatcgcgat gtcggcccgg gaaaccccta gtgcaccgac
     2461 accgggacgc ggccaaaatc acccctctcg cggcaggcac cgtcatggtg ccgcgcagga
     2521 cgggggctga ggagacagag accacgcgcc gcggcgatcc gccgacgagc gcgggacacg
     2581 caggccgcag ttcgggccga ggcagcatgc gaaagggcgt cagggttgac ctgacgccct
     2641 tttccatggg cgcacgccgc cgaacagcgc cgcgagcacc ccgctgttcg tcgaattgcc
     2701 aagcttgtcg cgaacctagc atcgggcgtc acccgcttcg tcccgaaaga ccgccatgcc
     2761 tgtcgacccc aaactgcgct cgctgaacat cgacgtcccg gcccagccgg agtcgctggt
     2821 caagctgtcg ctgctgctgg ccgacgacaa cgtcaacctg cagtcggtga gcgcgctgat
     2881 ctcgggcgac atggcgctgg cctcggcggt gctgaaggcg gtcaactcgt cgatgtatgg
     2941 gctgcgcggt cgcgtgcagt cggtgcagca ggcgatcacc cacctgggca cgcgcgaggt
     3001 cgccgcggtc accttcgcga tggggctgcg cgcggtgttc ccgcatgcgc ccgagctcga
     3061 gccggtgtgg gagcgcgccg gcgtgcgtgc gctgctgatg agccgcatcg gccagcaggt
     3121 cggcgtcgac ccgtggatcg cgcattcggc cggcctgttc gaggagtgcg gcaaggccgt
     3181 gctgttccgc cacgccaccg agcgctaccg cgccctgctc gccaaggcgg ccgacgacga
     3241 agaactgctg atgctcgagc acaccgagtt cggcgtcagc cacgacgccc tgggcgcggc
     3301 gctgtgcgag agctggggtc tggcggctgc cgcggtgacc agcgtgcgct ttcacgtcat
     3361 cgtcaacagc acacgcgagc tgccgatgca ggtgcccaag cgcacgatct gcgcgctgtc
     3421 ggcactggcg tacgcgctga tgaccgaccc ggccgcgctc gacgaggtcg cgcacgcgat
     3481 cgcgccgcag gccgggctgg acccgacgct ggtgctgcgc ggtgcgcgca aggtgcagga
     3541 gcagatcgag gcggcggtcg cgcgctccgc cgctgcggcg ggctgaagcg ccgcccgcgt
     3601 ccgccgcgac tcggggctgc ccttgacccg gccggcccgg aataccaccg cggcggcgcc
     3661 ggcagcactt cggccctaca gttccgcgtc ggcgcatggc gccgcgcaag gaacccccag
     3721 cgatgcagac ccgacgagag atgatgaggc gcagcgtcgc cgtggccgcg ctgctgggcg
     3781 gcgcggg
//
LOCUS       DATMJB010000622         2374 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_803284, whole genome shotgun sequence.
ACCESSION   DATMJB010000622 DATMJB010000000
VERSION     DATMJB010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803284"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VNU48_13170"
     CDS             <1..251
                     /locus_tag="VNU48_13170"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HWI12277.1"
                     /translation="PDYRVAVEVRSFESTLGEVAAIDAVWTVRRMKDRKTQTGQTAVR
                     ERIAEPGYEALAAAHSRAAAKLSQDIADAIRQLQRDAP"
     gene            306..965
                     /locus_tag="VNU48_13175"
     CDS             306..965
                     /locus_tag="VNU48_13175"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWI12278.1"
                     /translation="MQPDEIGRIIGDAILERRLAPGRKLNELELSKLLGVSRSLVHLA
                     LLGLHHDGLVSFEVNRGAFVARPTLDEAHFLFEAIAANERSAVDIVLAAPALPPLDGL
                     RSVHNKQVKAHAKGQMRSSEQLAIDFHRELIALTGNPLLIDAQRKLLLRYRIVTAIFK
                     TELDYCELEDHHGELIQLLQKSGSAARLRRLIDTHWRLVIRGHLSVQPGVEDLSEALR
                     L"
     gene            complement(1012..2007)
                     /locus_tag="VNU48_13180"
     CDS             complement(1012..2007)
                     /locus_tag="VNU48_13180"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWI12279.1"
                     /translation="MIERSLWHPVASAPDLGEAPMAVHLLDEPLVLWRDAAGAVHAFV
                     DRCPHRGARLSLGCVRGGELECPYHGWRFEGSGRAVAIPALPGFVPPPAHRVAAHAVR
                     EHCGLLWVRLDGTPAGDRSPPAFAAEQDPRLRKLTCGPYDVATSAPRLIENFLDMAHF
                     AFVHEDWLGDRAHMAIDDYRIDATENGFLATGCKAWQPRSSVHAEGGAMVEYTYEVNA
                     PYTAVLCKVPDAASVGIDGFRESIAMFVCPVSAESSRVWFRLAMNDFESPDETLQAFQ
                     DTIFGQDRPVLESQSPRRLPISGHAGAIELHSAADRSSAAYRRYLRECGIGFGVC"
     gene            complement(2078..>2374)
                     /locus_tag="VNU48_13185"
     CDS             complement(2078..>2374)
                     /locus_tag="VNU48_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWI12280.1"
                     /translation="AVDDVLNKTWKTGATKWGTKEGMNDFVKIAASVPQDTKKLVVQA
                     KAGLKNGTLAVFKGPLFANTGKEVLAKDKVADDEWNSKVTFYVQGVEGKSPAAK"
BASE COUNT          391 a          841 c          778 g          364 t
ORIGIN      
        1 atcccgacta ccgcgtggcc gtcgaggtgc gcagcttcga atcgacactc ggcgaggtgg
       61 cggctatcga tgccgtctgg acggtccgcc gcatgaagga tcgcaagacg cagaccgggc
      121 agacggcggt gcgcgagcgc atcgccgagc ccggctacga ggcgctcgcc gccgcgcaca
      181 gccgagcggc cgccaagctg agccaggaca tcgccgacgc catccggcag ttgcagcgcg
      241 acgccccatg acgcgccggg cgcgcaaaca gaccgtcggg tcaaccaagt ccaaaccgga
      301 tcgacatgca gccagatgag atcgggcgaa tcattggcga cgcgatcctg gagcggcggc
      361 tcgccccggg ccgcaagctc aatgagctcg agttgtccaa gctcctgggg gtcagccgtt
      421 cgctggtgca tctcgcgctg ctggggctgc atcacgacgg cctggtttcg ttcgaggtca
      481 accggggtgc cttcgttgcc aggccgaccc tggacgaggc ccatttcctg ttcgaagcca
      541 tcgccgcgaa cgagcggtcc gcggtcgaca tcgttctcgc agcgccggcc ttgccgccgc
      601 tggacggcct gcggagcgtt cacaacaaac aggtcaaggc gcatgcaaag ggtcagatgc
      661 gctcgtcgga acaactggcg atcgacttcc accgcgagct gatcgcgctc acgggcaacc
      721 ccctgctgat cgatgcacag cgaaagctgc ttctccggta ccggatcgtc acggccatat
      781 tcaagaccga gctcgactac tgcgagctgg aggaccacca cggcgaactg atccagcttc
      841 tgcaaaaaag cggatcggcc gcgcgcctgc ggcgtctcat cgacacccac tggcggctcg
      901 tgatccgcgg gcacctcagc gttcagccgg gcgtcgagga cctgtccgag gcgctgcgcc
      961 tgtgagcggc gccgcgtggt gcggcccagg cgccgcgggc cgaacccgtg atcagcacac
     1021 gccgaatccg atgccgcact ctcgaaggta gcgccgatag gcggccgagc tgcggtcggc
     1081 cgcgctgtgc aattcgatcg cgcccgcgtg cccggaaatc ggcaggcgcc tgggcgattg
     1141 cgattcgagc accggccggt cctggccgaa gatcgtgtcc tgaaaggctt gcagcgtctc
     1201 gtccggcgac tcgaagtcgt tcatcgccag gcggaaccac acgcggctcg attccgcgga
     1261 aaccgggcag acgaacatcg cgatcgactc gcgaaagccg tcgatgccga cgctcgcggc
     1321 atccggcacc ttgcacagca ccgcggtgta gggcgcgttc acttcatacg tgtactcgac
     1381 catcgcaccg ccctcggcat ggacgctcga acgcggctgc catgccttgc agccggtcgc
     1441 gagaaatccg ttctcggtcg cgtcgatgcg atagtcgtcg atcgccatgt gcgcgcggtc
     1501 gccgagccag tcttcgtgga cgaatgcgaa gtgcgccatg tcgagaaaat tctcgatgag
     1561 gcgcggcgca ctcgtcgcca cgtcgtaggg gccgcaggtc agcttgcgca ggcgcgggtc
     1621 ctgctcggcg gcgaatgccg gcgggctgcg gtcgccggcc ggcgtgccgt cgagccgcac
     1681 ccacagcagc ccgcaatgct cgcgcacggc gtgcgccgcg acgcggtggg ccggcggcgg
     1741 tacaaagccc ggcagcgccg gaatcgccac ggcccgaccg ctgccctcga agcgccagcc
     1801 gtggtagggg cactccagct cgccgccgcg cacacaaccc agcgagagcc gcgccccgcg
     1861 atgcgggcag cggtcgacga acgcgtgcac ggcgccggcc gcatcgcgcc agagcaccag
     1921 cggttcatcc aggagatgca ccgccatcgg cgcttcgcca aggtcgggag cgctcgccac
     1981 cggatgccaa agggaacgtt cgatcatcct cgctccaaca cgagcgtgcg cgcgccccgc
     2041 accacggcgc ggctggccgc cgcaggcatc gcgccgctca cttggcggcc ggcgacttgc
     2101 cctccacacc ctggacgtag aaggtgacct tgctgttcca ctcgtcgtcg gcgaccttgt
     2161 ccttcgcgag cacttccttg ccggtattgg cgaacagcgg ccccttgaac acggcgagcg
     2221 tgccgttctt caggccggcc ttcgcctgga cgaccagctt cttcgtgtcc tgcggcacgc
     2281 tggcggcgat cttgacgaag tcgttcatgc cctccttcgt gccccacttc gtggcccccg
     2341 tcttccacgt cttgttgagc acgtcgtcga ccgc
//
LOCUS       DATMJB010000623         3049 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_323312, whole genome shotgun sequence.
ACCESSION   DATMJB010000623 DATMJB010000000
VERSION     DATMJB010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323312"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1356
                     /gene="cobS"
                     /locus_tag="VNU48_13190"
     CDS             352..1356
                     /gene="cobS"
                     /locus_tag="VNU48_13190"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HWI12281.1"
                     /translation="MTTAAMTKTSTAAPASVPDMKVSVRQVFGIDSDLEVPAYSEVDP
                     HVPDVDSDYRFDRATTLAILAGFAHNRRVMVTGYHGTGKSTHIEQVAARLNWPCVRVN
                     LDSHISRIDLVGKDSIVVRDGKQVTEFRDGILPWALQHNIALVFDEYDAGRPDVMFVI
                     QRVLEVSGRLTLLDQNKVIKPHPAFRLFSTANTIGLGDTSGLYHGTQQINQGQMDRWS
                     IVTTLNYLAHDEEVEIVLAKAHHYRTQEGRDIVNKMVRLADLTRNAFANGDLSTVMSP
                     RTVITWAENADIFSDIGFAFRVTFLNKCDELERTLVAEFYQRCFNAELTESSVNVALS
                     "
     gene            1417..>3049
                     /gene="cobT"
                     /locus_tag="VNU48_13195"
     CDS             1417..>3049
                     /gene="cobT"
                     /locus_tag="VNU48_13195"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HWI12282.1"
                     /translation="MTTSNSKFRTGPKEAPTEPFKRAVTSCLRAIAKAPELEVTFAAE
                     RPGLAPGKARLPEPARKMTKRDAAIVRGHADSMALKLACHDPKVHRKLMPGNPQARGV
                     FEAVEQARVEAIGAKRMAGVAKNLTAMLDDHFHRGKFDEITDRADAPLSDALAMLVRE
                     RLTGLAPPAAARKMVDLWRPALEDKIGPRLDQLSLVTEDQAKFGDLVHDLLSALDLGD
                     DRNADADDENQDENSDGENDQSGSEGSPDSDGEQEMSAEQAQASTDEMSESAMESAQA
                     SASDAFDDGELGDDETPGEATRPNSRGGNEPRGPEYHAFAPKFDEVIAAEDLCDHDEL
                     ERLRSYLDKQLAHLQGIVARLANRLQRRLMAQQNRAWEFDLEEGILDPARLSRVVTDP
                     YHPLSFMHEKEATFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCGVKVEIL
                     GFTTRAWKGGQSREAWLAAGKPANPGRLNDLRHIIYKSADAPWRRARKNLGLMMREGL
                     LKENIDGEALIWAHNRLLARSEQRRILMVISDGAPV"
BASE COUNT          586 a          978 c          978 g          507 t
ORIGIN      
        1 catgggctga cacgaacgcg atcaatggat cgaggaagga agccacaaaa ttcctgtgcg
       61 aatgccggcg ccgggggctg gcctaacaca agtcccggcc ggggtggaaa gtgccgctgg
      121 cggttgcctg ttttttccga tccgcggtgg aattgccgaa acgccggcat gattcgcagg
      181 gcagccttcc aaaatccgag tccagagcct atcttgatga tatctcaccg gaactttgat
      241 tgagcggcgg gcttctgccg ggcgttgctc tctgataggt tcctgaaagc acccatccgc
      301 agccaataag caacatctgg tttcgggagc gtccgagacc tcggaggact gatgacgacc
      361 gccgccatga ccaagacttc caccgcagcg cccgccagtg tccccgacat gaaggtgtcg
      421 gttcggcagg tgttcggcat cgacagcgac cttgaggtgc cggcctattc cgaagtcgat
      481 ccgcatgtgc cggatgtcga ctcggactat cgcttcgatc gcgccaccac gctcgccatt
      541 ctcgccggct tcgcgcacaa ccgccgcgtc atggtcaccg gctatcacgg caccggcaaa
      601 tcgactcata tcgagcaggt cgcggcgcgg ctcaactggc cgtgcgtgcg cgtcaacctc
      661 gacagccaca tcagccgcat cgatctggtc ggcaaggact ccatcgtcgt gcgcgacggc
      721 aagcaggtca cggaattccg cgacggcatc ctgccgtggg cgctgcaaca caatatcgcg
      781 ctggtgttcg acgaatatga cgccggccgc cccgacgtga tgttcgtgat ccagcgcgtg
      841 ctggaagtct ccggccgcct gacgctgctc gaccagaaca aggtgatcaa gccgcatccg
      901 gcgttccggc tgttctcgac cgccaacacc atcggcctcg gcgatacttc gggcctctat
      961 cacggcaccc agcagatcaa ccagggccag atggaccgct ggtcgatcgt caccacgctg
     1021 aactatctgg cgcatgacga ggaagtcgaa atcgtgctgg ccaaggcgca tcactaccgc
     1081 acgcaggaag gccgcgacat cgtcaacaag atggtgcggc tcgccgatct cacccgtaac
     1141 gctttcgcca atggcgatct gtcgacggtg atgagcccgc gcaccgtgat tacctgggcc
     1201 gagaacgccg atatcttctc cgacatcggc tttgcgttcc gcgtcacctt cctcaacaag
     1261 tgtgacgagc tggagcggac gctggtcgcc gaattctatc agcgctgctt caacgccgaa
     1321 ctgacggagt cgtcggtcaa cgtggcgctt tcctgatagc gggcgccgct ggctatgggt
     1381 ccctgctttc gcagggacga cggcgctgag gttgaaatga cgacgtcgaa ttccaaattc
     1441 cgtaccggac ccaaggaagc gccgaccgag cctttcaagc gcgcggtgac gtcgtgcctg
     1501 cgcgcgatcg ccaaggcgcc cgagcttgaa gtgacgttcg ccgccgagcg cccgggtctt
     1561 gcgccgggca aggcgcggtt gcccgaaccg gcgcgcaaga tgacgaaacg cgacgccgcc
     1621 atcgtgcgcg gccatgccga ttcgatggcg ttgaaactcg cctgtcacga tccgaaagtt
     1681 caccgcaagc tgatgccggg aaatccgcag gcgcgcggcg tgttcgaggc ggtggagcag
     1741 gcgcgggtgg aggcgatcgg cgccaagcga atggcgggcg tcgccaaaaa cctcaccgcg
     1801 atgcttgacg atcatttcca tcgcggcaag ttcgacgaga tcactgaccg cgccgacgcg
     1861 ccgttgtcgg atgcgctggc gatgctggtg cgcgaacggc tgaccggcct ggcgccgccg
     1921 gcggctgcgc gaaagatggt cgacctgtgg cggccggcgc tggaagacaa gatcggcccg
     1981 cggctcgatc aactgagcct ggtgaccgag gaccaggcca aattcggcga cctcgtgcac
     2041 gatctgttgt cggcgctcga tctcggcgac gaccgcaacg ccgatgccga tgacgagaac
     2101 caggacgaga atagcgacgg cgagaacgac cagtccggct ctgagggctc gcccgacagc
     2161 gacggggagc aggaaatgag cgccgagcag gcgcaggctt ccaccgacga gatgtcggaa
     2221 agcgcgatgg aaagcgcgca ggcctccgcc agcgacgcct ttgatgacgg cgaactcggc
     2281 gacgacgaga ccccgggtga ggcgacacgt ccgaattcgc gcggcggcaa cgagccgcgc
     2341 gggccggaat atcacgcctt cgcaccgaaa ttcgacgagg tgatcgcggc cgaagacctc
     2401 tgtgaccacg acgaactgga acggttgcgc agctatctcg acaagcaact cgcgcatctg
     2461 caaggcatcg tcgcccgtct ggccaaccgc ttgcagcgcc gcctgatggc gcagcagaac
     2521 cgcgcctggg aattcgacct cgaagagggc attctcgatc cggcgcggct gtcgcgcgtg
     2581 gtcaccgatc cctatcatcc gctgtccttc atgcacgaga aggaagccac cttccgcgat
     2641 accgtggtga cgttgctgct ggacaattcc ggttcgatgc gcggccgtcc gatcacggtg
     2701 gcggcgacct gcgccgacat cctggcgcgg acgctggagc gttgcggcgt caaggtcgag
     2761 attctcggct tcaccacgcg ggcttggaag ggcgggcaat cgcgcgaggc gtggctggcc
     2821 gccggcaagc cggccaatcc cggccgtctc aacgatctgc gccacatcat ctacaaatcc
     2881 gccgacgcgc cgtggcgccg tgcgcggaaa aatctcgggc tgatgatgcg cgaggggctg
     2941 cttaaggaga acatcgatgg cgaggcgttg atctgggcgc ataaccgcct gctcgcccgc
     3001 tccgagcagc gccgcatcct gatggtgata tcggacggcg cgccggtcg
//
LOCUS       DATMJB010000624         3227 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_858726, whole genome shotgun sequence.
ACCESSION   DATMJB010000624 DATMJB010000000
VERSION     DATMJB010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858726"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VNU48_13200"
     CDS             <1..594
                     /locus_tag="VNU48_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI12283.1"
                     /translation="RRAIKLLQETGIPAAQARIDQYPHQFSGGMRQRVVIALALAAEP
                     KLIVADEPTTALDVSVQAQIISLLRRLCKEHGAAVMLVTHDMGVIAETCDRVAVMYAG
                     RIAEIGPVHDVIHAPAHPYTVGLMGSIPAMDEDRERLLQIDGAMPRLTAIPAGCAFNP
                     RCPKVFDRCRVERPDLLPAGATRAACWLHAPALRGAA"
     gene            591..1625
                     /locus_tag="VNU48_13205"
     CDS             591..1625
                     /locus_tag="VNU48_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HWI12284.1"
                     /translation="MSKVAGATAPLVEVHDLAKTFDVSAPWLNRVVERKPRQFVHAVD
                     GVSFSIQRGKTLALVGESGCGKSTIARLLVGLYAPTKGRVEFDGTDTATTFRSKHALA
                     LRRRMQMIFQDPYASLNPRWKVADIVGEPLKEHGLLSGKSATQGDKREMKAKVVQLLQ
                     SVGLAAADVEKFPHQFSGGQRQRISIARALATQPEFLVCDEPTSALAVSVQAQVLNIM
                     KDLQRRQGLTYLFISHNLAVVRHVSDQVGVMYLGRVVELADKADLFARPRHPYTRMLL
                     DAIPDIHMSGRARTPVQGEVPNPLNPPSGCSFNPRCPHANERCKQERPPLQTFGGALI
                     ACHAVEEGRI"
     gene            complement(1622..2905)
                     /gene="lysA"
                     /locus_tag="VNU48_13210"
     CDS             complement(1622..2905)
                     /gene="lysA"
                     /locus_tag="VNU48_13210"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWI12285.1"
                     /translation="MTLPGAPYIAYRGDDLFIEDRSVADLARAHGTPLYVYSRSAMLA
                     ALASYERALAGRDHLICYAMKANSSLAVLQTFAQAGCGFDIVSGGELERVLACGVPAS
                     RVVFSGVGKTRAEMRRALEVGVMCFNVESEAELELLSQVASSAGRIARVSLRVNPDVD
                     AGTHPYISTGLKGNKFGIAHQDALANYRRAAALPGLQVVGIDCHIGSQITDAGPYLDA
                     LDRLLELIESIEAHGIAIHHLDLGGGLGITYTDETPPSAEALVTSLLARIDARGHGHR
                     KIMFEPGRSLVGNAGALVSEVLYVKPGEQKNFCIVDAAMNDLMRPAMYQAWMRIVACR
                     RRDEPPMNFDVVGPVCESGDWLGKDRALAVRAGDFVAVLSAGAYSMSMASNYNTRGRA
                     AEVMVAGDRAWLIRDREQASELFRGEHLLPADAGH"
BASE COUNT          505 a         1122 c         1124 g          476 t
ORIGIN      
        1 cggcgcgcga tcaagctgct gcaggagacc ggcatcccgg cggcgcaggc gcgcatcgac
       61 cagtacccgc accagttctc gggcggcatg cgccagcgcg tggtgatcgc gctcgcgctc
      121 gcggccgagc cgaagctgat cgtcgccgac gagccgacga cggcgctcga tgtctcggtc
      181 caggcgcaga tcatttcgct gctgcggcgc ctgtgcaagg agcacggcgc cgcggtgatg
      241 ctggtcacgc acgacatggg cgtgatcgcc gagacctgcg atcgcgtggc cgtgatgtac
      301 gccggccgca tcgccgagat cggcccggtg cacgacgtca tccacgcacc ggcgcacccg
      361 tacaccgtcg ggctgatggg ctcgatcccg gcgatggacg aagaccgcga gcggttgctg
      421 cagatcgacg gcgcgatgcc gcgcctgacc gccattccag ccggctgcgc gttcaacccg
      481 cgctgcccga aagtgttcga ccgctgccgc gtcgagcgac ccgacttgct gcccgccggc
      541 gcgacacgcg ccgcctgctg gctgcacgcg ccggcgctgc ggggggcagc atgagcaagg
      601 tcgcgggcgc aacggcgccg ctggtcgagg tgcacgatct ggcgaagacc ttcgatgtct
      661 cggcgccgtg gctgaaccgc gtggtcgaac gcaagccgcg ccaattcgtg catgcggtcg
      721 atggcgtgag cttctcgatc cagcgtggca agacgctcgc attggtcggt gaatcgggct
      781 gcggcaagag cacgatcgcg cgcctgctgg tcggcttgta cgcgccgacg aagggccgcg
      841 tcgaattcga cggcaccgac accgcgacga cgtttcgatc taagcatgcg ctggcgctgc
      901 gccggcgcat gcagatgatc ttccaggacc cgtacgcgag cctgaacccg cgctggaagg
      961 tggccgacat cgtcggcgag ccgctgaagg agcatggcct gctcagcggc aagagcgcga
     1021 cccagggtga caagcgcgag atgaaggcga aggtcgtgca gctgctgcag tcggtcggcc
     1081 tcgcggctgc cgatgtcgag aaattcccgc accagttctc cggcggccag cgccagcgca
     1141 tctcgatcgc gcgtgccctg gccacacagc ccgagttcct ggtgtgcgac gagccgacct
     1201 cggcgctcgc cgtttcggtg caggcgcagg tgctcaacat catgaaggac ctgcagcgca
     1261 ggcagggcct gacgtatttg ttcatctcgc acaacctggc ggtcgtgcgc catgtgtcgg
     1321 accaggtcgg cgtgatgtac ctgggccgcg tggtcgaact cgccgacaag gccgatctgt
     1381 tcgcgcgccc gcggcacccg tacacgcgca tgctgctcga cgcgatcccc gacatccaca
     1441 tgagcggccg tgcgcgcacg ccggtgcagg gcgaggtgcc gaacccgctg aacccgccga
     1501 gcggctgcag cttcaacccg cgctgcccgc atgcgaacga gcgctgcaag caggagcggc
     1561 cgccgctgca aacattcggc ggcgcgctga tcgcgtgcca cgcggtcgag gaaggccgga
     1621 tctagtggcc ggcgtccgcg ggcagcaggt gctcgccgcg aaacaattcg ctggcctgtt
     1681 cgcggtcccg gatcagccaa gcgcgatcgc ccgccaccat gacctcggcg gcccggccgc
     1741 gcgtgttgta gttgctggcc atgctcatgc tgtacgcacc ggccgacagc acagcgacga
     1801 agtcgcccgc gcgcaccgca agggcgcgat ccttgccgag ccagtcgccg gattcgcaga
     1861 ccgggccgac gacatcgaag ttcatcggcg gctcgtcgcg ccgccggcag gcgacgatgc
     1921 gcatccaggc ctggtacatc gccgggcgca tcaggtcgtt cattgccgcg tcgacgatgc
     1981 agaagttctt ctgctcgccg ggcttcacgt acagcacctc gctgaccagc gcgccggcgt
     2041 tgccgaccag cgagcggccg ggctcgaaca tgatcttccg gtggccgtgg ccgcgcgcat
     2101 cgatgcgcgc gagcaggctc gtcaccagcg cttcggcgga cggcggcgtc tcgtcggtgt
     2161 aggtgatgcc caggccgccg ccgagatcga ggtggtgaat cgcgatgccg tgcgcctcga
     2221 tcgattcgat cagttcgagc aggcggtcga gcgcgtcgag gtacgggccg gcgtcggtga
     2281 tctgcgagcc gatgtggcaa tcgatgccca ccacctgcag cccgggcaag gcggcagcgc
     2341 gtcggtagtt cgccagcgcg tcctggtgcg cgatgccgaa cttgttgccc ttcaggccgg
     2401 tcgagatgta ggggtgcgtg ccggcgtcca cgtccggatt cacgcgcagg ctgacacggg
     2461 cgatgcgacc ggcgctgctc gccacttgcg acagcagctc gagttcggcc tcgctctcga
     2521 cgttgaagca catcacgccg acctcgagcg cgcggcgcat ctcggcgcgc gtcttgccga
     2581 cgccggagaa cacgacccgg ctcgccggca caccgcaggc gagcacgcgc tcgagttcgc
     2641 cgccggagac gatgtcgaag ccgcagccgg cctgcgcgaa ggtctgcagc acggcaaggc
     2701 tcgagttcgc cttcatcgcg tagcagatca ggtggtcgcg cccggcgagc gcgcgctcgt
     2761 agctcgccag cgccgccagc atcgcgctgc gcgaatacac gtacagcggc gtgccgtgcg
     2821 cgcgcgcgag gtcggcgacg ctgcggtcct cgatgaacag gtcgtcgccg cggtaggcga
     2881 tgtacggcgc gccgggcaag gtcatggggc ggacgccgcg gcgggcgcgg acgcggcgcc
     2941 ggccggcttg ggcagcgtca gcggcccctt ctgaccgcag gccgcgccca gcgccgcggc
     3001 gaccagacat gcacttactg tcgacgcagc cttcgcggcc gccgctacac tgatcttgcg
     3061 ctgcaacaac atccctggat tctatcgacc atgtccgccg accttcaccc cagcccgctg
     3121 accgacagcg actacgacgc caagacgcgc gcggtgctgg ccagcgtcga ggcgagcgtc
     3181 gatgcctggc tgcaggacga cgtggtcgac atcgacacgc acagatc
//
LOCUS       DATMJB010000625         2817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_67150, whole genome shotgun sequence.
ACCESSION   DATMJB010000625 DATMJB010000000
VERSION     DATMJB010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67150"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VNU48_13215"
     CDS             complement(<1..226)
                     /locus_tag="VNU48_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HWI12286.1"
                     /translation="MTSFEALADRVGRLMREVAAEIIVPRFRALKTGDVMEKSPGEVV
                     TIVDHEAERRIEPVLLALRRGSRVVGEEGVA"
     gene            372..1139
                     /gene="aat"
                     /locus_tag="VNU48_13220"
     CDS             372..1139
                     /gene="aat"
                     /locus_tag="VNU48_13220"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HWI12287.1"
                     /translation="MITWLHAERDALPETRSALPRGSDAPGLLAAGGELTPARLTEAY
                     SKGVFPWYSEGQPVLWWSPDPRMVLWVDEFKLSRSLRKTVRAFVESPNGELRVDGAFR
                     AVISACAATPRDGQDGTWIVPEMIDAYCRWHELGRTHSFETWIDGRLVGGLYGVSIGR
                     MFFGESMFSHRTDASKIAVAALICFCREHAITLIDCQQHTTHLASLGARELPRREFER
                     ELGPRLGQPPVRDWTYHRSMWRHLAPAATAAPKDPRA"
     gene            1136..1909
                     /locus_tag="VNU48_13225"
     CDS             1136..1909
                     /locus_tag="VNU48_13225"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HWI12288.1"
                     /translation="MTHPKELPLASLQFYATAPYPCSYLEGRLARSQVATPSHLIHAD
                     AYSGLVANGFRRSGMFTYRPYCDGCKACLPLRIPVARFVPTRSQRRAQRAHGRLEARV
                     LRLCFVPEHYQLYLRYQSGRHLGGGMDHDSVDQYTQFLLQSRVNSRLVEFREPSTPAG
                     PGKLKMVSILDVLNDGLSAVYTFYEPEPHTSYGTYNVMWQIEQTRLLKLPHVYLGYWI
                     EQSGKMAYKAQFRPHELLIDGHWQAPATSDAAGATPPAG"
     gene            complement(1890..>2817)
                     /locus_tag="VNU48_13230"
     CDS             complement(1890..>2817)
                     /locus_tag="VNU48_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI12289.1"
                     /translation="LCVAGRGQLYAYCASHGVAHKRCGKLIVAANAAQAAKVAQIQAQ
                     AARNGVHDLRALDATETRALEPELAAVAALHSPSTGIVDSHGLMLALQGDLQAHGGVL
                     ALRSPALSVTADDSGFVVEVGGDTPMTLAASRVVNAAGLWAPGFAASIAGLAPGLVPQ
                     ARYAKGNYFALASRAPFSHLIYPIPEEAGLGVHLTLDLAGQARFGPDVEWISPATPDA
                     IDYRVDAQRAAAFEAEIRRYWPGLPAGALQPAYSGVRPKLQGEGAAAQDFVLQGPAEH
                     GLAGLLNLYGIESPGLTASLALADAALSRLGV"
BASE COUNT          423 a         1032 c          951 g          411 t
ORIGIN      
        1 cagccacgcc ttcctcgccg accacacgcg agcctcggcg cagcgcgagc aacaccggct
       61 cgatgcggcg ctcggcttca tggtcgacga tggtcaccac ctcgccgggc gacttctcca
      121 tcacgtcgcc ggtcttcaac gcccggaagc gtggaacgat gatctcggcg gcgacctcac
      181 gcatcaggcg gccgacacga tctgccaggg cttcgaacga cgtcatgcgc cgagtatccg
      241 tgccgcacgg ggacgggcgc ggcgtcgggg gaacggcgaa ggactcacgc ggcgatgttg
      301 gcggtccatg cacaggggac tcgaggtgcc gtgtacgatt catcggcacg gacctccgcc
      361 gaccctcgtc catgatcacc tggctccacg ccgaacgcga tgcactgccc gagacgcgca
      421 gcgctttgcc gcgcggctcc gatgcgccgg gtctgctcgc cgccggcggc gagctcacac
      481 cggcgcgtct gaccgaggcc tattcgaagg gcgtgtttcc ctggtacagc gaaggccagc
      541 cggtgctgtg gtggtcgccc gatccacgca tggtgttgtg ggtcgacgag ttcaagctgt
      601 cgcgctcgct gcgcaagacg gtgcgcgcgt tcgtcgagtc gccgaacggc gagttgcgcg
      661 tcgacggcgc cttccgcgcc gtcatcagcg cctgcgccgc aacgccgcgt gacgggcagg
      721 atggcacctg gatcgtgccg gagatgatcg atgcctactg ccgctggcac gaactcggcc
      781 gcacccacag cttcgagacc tggatcgacg ggcgcctcgt gggcgggctc tacggcgtga
      841 gcatcggccg catgttcttc ggcgagtcga tgttctcgca ccgcaccgat gcgtcgaaga
      901 tcgcagtcgc cgcgctgatc tgcttctgtc gcgagcacgc catcacgctg atcgactgcc
      961 agcagcacac gacccacctg gcctcgctcg gtgcgcgcga actgccgcgc cgggagttcg
     1021 aacgtgaact cggccctcgt ctcgggcagc cgccggtgcg ggattggacc tatcatcgat
     1081 cgatgtggcg gcacctcgca cctgccgcaa ctgcagcgcc gaaggaccct cgcgcgtgac
     1141 ccacccgaaa gaactcccgc tcgcgtcgct gcagttctac gcgactgctc cgtacccctg
     1201 cagctatctc gagggccgcc tggcgcgctc gcaggtcgcc acgcccagcc acctgatcca
     1261 cgccgacgcc tattcggggc tggtcgcgaa cggctttcgg cgcagcggca tgttcaccta
     1321 ccggccctac tgcgacggct gcaaggcctg cctgccgctg cgcatcccgg tggcgcgctt
     1381 cgtgccgacg cgcagccagc ggcgcgccca gcgcgcgcac ggccggctcg aggcgcgcgt
     1441 gctgcgcctg tgcttcgtgc ccgagcacta ccagctctac ctgcgctacc agtcgggccg
     1501 gcatctgggc ggtggcatgg accacgacag cgtcgaccag tacacccagt tcctgctgca
     1561 aagccgggtc aactcgcggc tggtcgagtt tcgcgaaccg tcgacgccgg ccgggccggg
     1621 caagctgaag atggtgtcga tcctggacgt gctcaacgac ggcctgtccg ccgtctacac
     1681 cttctacgag cccgagccgc acaccagcta cggcacctac aacgtgatgt ggcagatcga
     1741 gcagacgcgg ctgttgaagc tgccgcacgt ctacctcggc tactggatcg agcagagcgg
     1801 caagatggcc tacaaggccc agttccgccc gcacgagttg ttgatcgacg gccactggca
     1861 ggcgcccgcg accagcgacg ccgccggcgc tacaccccca gccggctgag cgccgcgtcg
     1921 gcgagcgcga ggctcgcggt cagacccggc gactcgatgc cgtacaggtt cagcaggccc
     1981 gccaggccgt gctccgcagg gccttgcagc acgaagtcct gggccgccgc gccttcgccc
     2041 tgcagcttgg gccgcacacc gctgtacgcc ggctgcagcg cgccggcggg cagcccgggc
     2101 cagtagcggc ggatctcggc ctcgaaggcc gccgcgcgtt gcgcgtcgac gcggtagtcg
     2161 atcgcgtccg gggtcgcggg cgagatccat tcgacatcgg ggccgaagcg cgcctggccc
     2221 gccagatcga gcgtcaggtg cacgcccagg ccggcctctt cggggatcgg gtagatcagg
     2281 tgcgagaacg gcgcgcgcga cgcgagcgcg aagtagttgc ccttcgcata acgcgcctgc
     2341 ggcacgagcc ccggcgccag cccggcgatc gacgcggcga agcccggcgc ccacagcccg
     2401 gccgcattga cgacgcggct ggcggccagc gtcatcggcg tgtcgccacc gacctcgacg
     2461 acgaagcccg agtcatcggc ggtgaccgaa agcgccggcg accgcagcgc cagcacgcca
     2521 ccgtgggctt gcagatcacc ctgcagcgcg agcatcaggc cgtggctgtc gacgatgccg
     2581 gtcgacggcg aatgcagcgc cgccacggcg gccagttcgg gctccagcgc gcgcgtctcg
     2641 gtggcgtcga gcgcgcgcag gtcgtgcacg ccgttgcgcg cggcctgcgc ctggatctgc
     2701 gcgaccttgg cggcctgcgc cgcgttcgcc gcgacgatca gcttgccgca gcgcttgtgc
     2761 gcgacgccgt gcgacgcgca gtacgcatac agctgcccgc gcccggccac gcacaac
//
LOCUS       DATMJB010000626         3631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_382811, whole genome shotgun sequence.
ACCESSION   DATMJB010000626 DATMJB010000000
VERSION     DATMJB010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382811"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..992)
                     /locus_tag="VNU48_13235"
     CDS             complement(72..992)
                     /locus_tag="VNU48_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWI12290.1"
                     /translation="MDRLKQLESFAAVATRGSLTAAAAAEGVAPAVIGRRIDALERRL
                     GVKLLVRTTRRISLTHEGSAFLEDCQRLLADWANAEASVSAGGVKASGHLRITSPAGF
                     GRRHVAPLVPRFLALHPEVSLSLNLSDRVVDIVNESFDCAVRVGDLPDSSLVSVRLAD
                     NRRLCVATPAYLKRAGTPMHPAELARHECLTLSSDASQTRGWAFLIDGEVRHLRPSGR
                     LDCTDGQVLHAWCAAGLGLAWRSTWEVEQEVNAGELVSVLDAFAAPPNGIYAVFPHAK
                     HLALRVRLWIDFLKHSYGDSAYWRRAPALK"
     gene            1071..1781
                     /locus_tag="VNU48_13240"
     CDS             1071..1781
                     /locus_tag="VNU48_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HWI12291.1"
                     /translation="MDSSPVSAAPRAVLFDAYGTLFDVYSVSLLAEQLFPGFGERLSV
                     LWRDKQIEYTRLTSMSGQYRPFWDLTRAGLRFAAQRLGLALDATSEDRLMNQYRHLSS
                     FPENREVLAELKARGIPAGILSNGDPEMLGVAVKSAGFSGLLAHVISVHALRKFKTDP
                     ATYALGTQALNLPAKDILFVSSNGWDAIGATWFGYTTLWVNRFDLPLEQLDTAPTRIG
                     TSLRDVLDFFPTSNASRP"
     gene            1778..>3631
                     /locus_tag="VNU48_13245"
     CDS             1778..>3631
                     /locus_tag="VNU48_13245"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HWI12292.1"
                     /translation="MTARTTPRTTVHGLQVATALHHFIEEQVLPGTGVAPAAFWQGFD
                     AIVRELAPKNAALLAERERLQAELDRWHRKHPGPIKDGAKYRAFLEKIGYLVPVPADV
                     KATTKNVDAELALQAGPQLVVPITNARYALNAANARWGSLYDALYGTDALPETDGADK
                     GSGYNPVRGAKVIEYARHVLDRCAPLASGSQLDSTGYAVKAGALVVTLSDGTHTGLAK
                     PAQFVGYQGAASAPSSVLLVHHGLYLDLRIDRGTPIGAGDPAGVADLVLESALSTILD
                     LEDSVAVVDADDKVLAYGNWLGILKGTLTEQVAKGGKTFTRGLNADRVYVGPKGGEVT
                     LHGRSLLFVRNVGHLMTNPAVLWGDRGHEIPEGILDAVVTTTIALHDLQRHGANGIVN
                     SRKGSVYIVKPKMHGPAEVAFASELFGRVEQLLGLPKATVKLGIMDEERRTSVNLKAC
                     IAAAADRVAFINTGFLDRTGDEMHTAMLAGPVLRKGDMKGSAWIAAYERNNVLVGLSC
                     GLRGKAQIGKGMWAMPDLMAAMLEQKIAHPKSGANTAWVPSPTAATLHALHYHQVDVF
                     AVQKSLEKTDAAAAAPAILEGLLSVPVVKKAKWKEAERQQELDNNAQGILGY"
BASE COUNT          592 a         1286 c         1231 g          522 t
ORIGIN      
        1 gtgggccact tcgtggcccc tggcggcccg gcgctcggcc cgccaccgcg gcgatccgcg
       61 cgcaggccgt ttcacttcaa tgccggcgcg cgcctccagt acgcgctgtc accgtagctg
      121 tgcttcagga aatcgatcca cagccgcacc cgcaacgcca ggtgcttggc gtgcgggaac
      181 actgcgtaaa tgccgttcgg cggcgccgca aacgcgtcga gcacgctgac gagttcgccc
      241 gcgttcacct cttgctcgac ctcccaggtc gagcgccacg ccaggcccag tccggccgca
      301 caccaggcgt gcaggacctg gccgtcggtg cagtccagcc gcccgctcgg gcgcaggtgg
      361 cgcacttcgc cgtcgatcag gaaggcccag ccgcgcgtct gactcgcgtc gctcgacaag
      421 gtcaggcatt cgtggcgcgc caactcggcc gggtgcatcg gcgtgccggc gcgcttcagg
      481 taggcgggcg tggccacgca cagccgtcgg ttgtcggcca gacgcacgct gaccaggctg
      541 ctgtcgggca ggtcgccgac gcgcaccgcg caatcgaagc tctcgttgac gatgtcgacc
      601 acgcggtcgg acaggttcag cgacaggctc acttcggggt gcagcgccag gaagcgtggc
      661 accagcggcg cgacatggcg gcgcccgaag ccggccggcg aggtgatgcg caggtggccg
      721 ctggccttga cgccaccggc gctgacgctg gcttcggcgt tggcccagtc ggccagcagg
      781 cgctggcagt cttcgaggaa ggcgctgccc tcgtgcgtca gcgagatgcg ccgcgtcgtg
      841 cgcaccagca gcttcacgcc caggcgccgc tcgagcgcgt cgatgcggcg gccgatcacc
      901 gccggcgcga cgccctcggc cgcggccgcg gcggtcaggc tgccgcgggt cgcgactgcg
      961 gcgaacgatt cgagctgctt caggcggtcc atgattcagt gcaatttggt catcaatcca
     1021 cggcgaagat cgtacttaat gtcggtgcgc ccatcgaata gatttgcgcc atggactcct
     1081 cgcccgtctc tgccgccccg cgcgcggtgc tgttcgatgc ctacggcacg ctgttcgacg
     1141 tctacagcgt ctcgctgctg gccgagcagc tgttccccgg tttcggcgag cgcctgtcgg
     1201 tgctgtggcg cgacaagcag atcgagtaca cgcgcctgac ctcgatgagc ggtcagtacc
     1261 gcccgttctg ggacctgacc cgcgccgggc tgcgcttcgc cgcgcagcgc ctgggcctgg
     1321 cgctggacgc gaccagcgag gaccggctga tgaaccagta ccggcacctc agcagcttcc
     1381 ctgagaaccg tgaggtgctg gccgagctga aggcgcgcgg catcccggcc ggcatcctgt
     1441 ccaacggcga ccccgaaatg ctcggcgtgg ccgtcaagag cgcgggcttc tcgggcctgc
     1501 tcgcgcatgt catcagcgtg cacgcgctgc gcaagttcaa gaccgacccg gccacctatg
     1561 cgctcggcac gcaggccctg aacctgcccg cgaaggacat cctgttcgtc tcgagcaacg
     1621 gctgggacgc gatcggcgcg acctggttcg gctacacgac gctgtgggtg aaccgcttcg
     1681 atctgccgct cgaacagctc gacaccgcgc cgacgcgcat cggcacaagc ctgcgcgacg
     1741 tgctcgactt ctttcccacc tcaaacgcct cgagaccatg accgcacgca cgactccacg
     1801 aacgacagtc cacggccttc aagtcgccac cgccctgcac cacttcatcg aggagcaggt
     1861 gctgcccggc accggcgtgg cgcccgccgc gttctggcag gggttcgacg cgatcgtgcg
     1921 cgagttggcg ccgaagaacg ccgcgctgct ggccgagcgc gaacggctgc aggccgagct
     1981 cgaccgctgg caccgcaagc acccggggcc gatcaaggac ggtgcgaagt accgtgcctt
     2041 cctcgagaag atcggctacc tcgtgccggt gcccgcggac gtgaaggcaa cgacgaagaa
     2101 cgtcgacgcc gaactggcgc tgcaggccgg cccgcagctg gtcgtgccga tcaccaacgc
     2161 gcgctacgcg ctgaacgcgg cgaatgcgcg ctggggttcg ctgtacgacg cgctctacgg
     2221 caccgacgcg ttgcccgaga ccgacggcgc cgacaagggc agcggctaca acccggtgcg
     2281 cggcgcgaag gtgatcgagt acgcgcgcca cgtgctcgat cgctgcgcgc cgctggcaag
     2341 cggttcgcaa ctggacagca ccggctacgc ggtgaaggcc ggcgcgctgg tcgtcacgct
     2401 gagcgacggc acgcacaccg gcctggcgaa gccggcacag ttcgtcggct accagggcgc
     2461 ggccagcgcg ccgtcgtcgg tgctgttggt ccaccacggc ctgtacctgg acctgcgcat
     2521 cgaccgcggc acgccgatcg gcgccggcga cccggccggc gtcgccgacc tggtgctcga
     2581 atcggcgctg tcgacaatcc tcgatctcga ggactcggtc gccgtcgtcg atgccgacga
     2641 caaggtgctg gcctacggca actggctcgg catcctgaag ggcacgctga ccgagcaggt
     2701 cgccaagggc ggcaagacct tcacgcgcgg gctgaacgcc gaccgtgtct acgtcggccc
     2761 gaagggtggc gaagtgacgc tgcacggccg ctcgctgctg ttcgtgcgca acgtcggcca
     2821 cctgatgacg aaccccgcgg tgctgtgggg cgaccgcggc cacgagatcc ccgaaggcat
     2881 cctcgacgcc gtcgtgacga cgacgatcgc gctgcacgac ctgcagcgcc acggtgccaa
     2941 cggcatcgtc aactcgcgca agggctcggt ctacatcgtc aagccgaaga tgcacggccc
     3001 ggccgaggtc gcattcgcga gcgaactgtt cggccgcgtc gagcaattgc tcgggttgcc
     3061 gaaggccacc gtcaagctcg gcatcatgga cgaggagcgc cgcaccagcg tgaatctgaa
     3121 ggcctgcatc gccgccgccg ccgaccgcgt cgcgttcatc aacaccggct tcctcgatcg
     3181 caccggcgac gagatgcaca ccgcgatgct cgccggcccg gtgctgcgca agggcgacat
     3241 gaagggcagc gcgtggatcg ccgcctacga gcgcaacaac gtgctcgtcg gcctgagttg
     3301 cggcctgcgc ggcaaggcgc agatcggcaa aggcatgtgg gcgatgcccg acctgatggc
     3361 cgcgatgctc gaacagaaga tcgcgcaccc gaaatccggc gccaacaccg cgtgggtgcc
     3421 gagcccgacg gccgcgacgc tgcatgcgct gcactaccac caggtcgacg tgttcgcggt
     3481 gcagaagtcg ctcgagaaga ccgatgccgc tgcggccgcg ccggcgatcc tcgaagggct
     3541 gctgagcgtg ccggtcgtca agaaggcgaa gtggaaagaa gccgagcgcc agcaggagct
     3601 cgacaacaac gcgcagggca tcctcggcta c
//
LOCUS       DATMJB010000627         2758 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_856467, whole genome shotgun sequence.
ACCESSION   DATMJB010000627 DATMJB010000000
VERSION     DATMJB010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856467"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VNU48_13250"
     CDS             <1..400
                     /locus_tag="VNU48_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HWI12293.1"
                     /translation="NAKRKTDVGIAAVSDVGRFTRKGEISGNINNDFSGVARTQRAGK
                     LLAQLTVTVVAIDPNGDLRIAGEQLLEVNDEKQMIRLEGKVRSEDIGDNNSVVSNRIA
                     DAKIHYVGDGVLADGQKPGLITRILTWLGL"
     gene            385..1533
                     /locus_tag="VNU48_13255"
     CDS             385..1533
                     /locus_tag="VNU48_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HWI12294.1"
                     /translation="MARAVIALLRGVALTTLLVSSSWAIVPKDAAPDGIRLKDLGKLG
                     GWRENAVVGYGLVTGLAGTGDSPRNQATRQSIANLLQHFDLKLTPDQVSSRNVAAVMV
                     TASLPPFAQRGDTLDVTVTSMGDARSLLGGSLLLAPLKGPDGRVYALAQGAISVGGYK
                     YDMNGNVVQKNHPTVGVIPSGAAIEVPLTADVVDRDNKLVFVLARPDYTTANRIAAAI
                     NRQLGDGTARARTAADVDIKPAQAQIDDLVAFMTSIENLSVEPDRRARVVINERTGVV
                     VSGGDVRISKVSISHGDLKVSIVTDNTVSQPAFVRQTDASVRTEVVSNSRVDVKESTS
                     TQFIAPTHNTISDLVQALAKLKTSTRDIISILQAVKAAGALHADLIIQ"
     gene            1559..1936
                     /locus_tag="VNU48_13260"
     CDS             1559..1936
                     /locus_tag="VNU48_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HWI12295.1"
                     /translation="MSNTTEAVTSTLMKLALDGASLRHQAIAANIANAGSPGYAPVRV
                     DFEQRLRQARERIEAGEAPNSVAADASATIENEPRGAAVSPGSIDLQTAQLAQNVVHY
                     QALVKGWGKRMAILSTAINEGKR"
     gene            1936..2379
                     /gene="flgC"
                     /locus_tag="VNU48_13265"
     CDS             1936..2379
                     /gene="flgC"
                     /locus_tag="VNU48_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HWI12296.1"
                     /translation="MDYTTAFQISAAGMTLERARVDVTAMNLANMHASRAPDGSMYQP
                     LRAVATSTSIASFGTVYDAAQGRSALTLPQDVHVEAMQVAPRMVHEPGHPHADDRGYV
                     AYPGVNHAGEMVNLVSALRSYEANVVAMNAAKTMALKALDIGGGT"
     gene            2438..>2758
                     /gene="fliE"
                     /locus_tag="VNU48_13270"
     CDS             2438..>2758
                     /gene="fliE"
                     /locus_tag="VNU48_13270"
                     /inference="COORDINATES: protein motif:HMM:NF014143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HWI12297.1"
                     /translation="MNSIERIDNYLPPMRPTDDLVPSDLAPASAPPLQGTSAFGQWFT
                     NQVEKVNTQIEQSERQLQAVATGEAQNLHQVMIDLEEARLSFQMLVQVRNRVLEAYQD
                     VMRKI"
BASE COUNT          517 a          944 c          918 g          379 t
ORIGIN      
        1 caacgccaaa cgcaagaccg atgtcgggat cgctgcggtg tcggatgttg gacgcttcac
       61 gcgcaaaggc gagatcagcg gcaacatcaa caacgacttc agcggcgtcg cgcgcaccca
      121 gcgcgccggc aagctgctgg cgcagctcac cgtcacggtc gtcgcgatcg atccgaacgg
      181 cgacctgcgc atcgccggcg agcagttgct cgaggtcaac gacgagaagc agatgatccg
      241 gctcgagggc aaggtgcgct ccgaggacat cggcgacaac aacagcgtgg tgtccaaccg
      301 catcgccgac gccaagatcc actacgtcgg cgacggtgtg ctggccgatg gccagaagcc
      361 cggcctgatc acgcgcatcc tgacatggct cgggctgtga tcgcgctgct gcgcggcgtc
      421 gcgctgacga ccctgttggt gagcagcagc tgggccatcg tgcccaagga cgcggcgccc
      481 gacggcatcc gcctcaagga cctgggcaag ctcggcggct ggcgagagaa cgcggtcgtc
      541 ggctacggcc tggtgaccgg cctggcgggg accggcgact cgccgcgcaa ccaggcgacg
      601 cgccagtcga tcgccaacct gctgcagcac ttcgacctca agctcacgcc cgaccaggtg
      661 agcagccgca acgtcgcggc ggtgatggtc accgcgagcc ttcccccatt cgcgcagcgc
      721 ggcgacacgc tcgacgtgac ggtcacctcg atgggcgatg cgcgcagcct gctcggcggc
      781 agcctgctgc tggcgccgct gaaggggccg gacgggcgcg tctacgcact ggctcagggg
      841 gcgatctcgg tcggtggcta caagtacgac atgaacggca acgtcgttca gaagaatcac
      901 ccgaccgtcg gcgtgattcc gtccggggcg gcgatcgagg tgccgctgac ggccgatgtc
      961 gtcgaccgcg acaacaagct cgtcttcgtc ctcgcgcggc ccgactacac gacggccaac
     1021 cgcattgcgg cggccatcaa ccggcagctc ggcgacggca cggcgcgtgc gcgcaccgct
     1081 gccgacgtcg acatcaagcc ggcgcaggcg cagatcgacg acctggtcgc gttcatgacc
     1141 tcgatcgaga acctctcggt cgaaccggat cgccgcgcgc gggtggtgat caacgagcgc
     1201 accggcgtgg tcgtctcggg cggtgacgtg cgcatctcca aagtatcgat ctcgcatggc
     1261 gacctgaagg tgtcgatcgt cactgacaac acggtctcgc agccggcctt cgtgcgccag
     1321 accgacgcca gcgtgcgcac cgaggtcgtc tccaacagcc gcgtcgacgt gaaggaatcg
     1381 acctcgacgc agttcatcgc gccaacccac aacacgatct cggatctggt gcaggcgctg
     1441 gccaagttga agaccagcac gcgcgacatc atttcgattc ttcaagcggt caaggcagcg
     1501 ggcgccctgc atgccgatct catcattcag taaccacaga caacaggagc gtgctgcgat
     1561 gagcaacacc accgaggccg tgacctcgac gctgatgaaa ctggcgctgg acggcgcgtc
     1621 gctgcgccac caggcgatcg cggccaacat cgcgaacgcc ggctcgccgg gttacgcgcc
     1681 ggtgcgcgtg gacttcgagc agcgcctgcg gcaggcgcgc gagcgcatcg aggctggcga
     1741 ggcgccgaac agcgtcgccg ccgacgcatc ggccacgatc gagaacgagc cgcgcggcgc
     1801 cgccgtgagc ccgggatcga tcgacctgca gaccgcgcag ctggcccaga acgtcgtgca
     1861 ctaccaggcc cttgtcaagg gctggggcaa gcgcatggcg atcctgtcga ccgcgatcaa
     1921 cgaaggcaag cgctgatgga ctacacgacc gccttccaga tcagtgccgc cggcatgacg
     1981 ctcgaacgcg cgcgcgtcga cgtgacggcg atgaacctgg ccaacatgca cgcgagccgc
     2041 gcgcccgacg gttcgatgta ccagccgctg cgagcggtcg cgacgtcgac ctcgatcgcg
     2101 tcgttcggca ccgtctacga cgccgcgcag gggcgctccg ccttgacgct gccgcaggac
     2161 gtgcatgtcg aggcgatgca ggtcgcgccg cgcatggtcc acgagcccgg gcacccgcat
     2221 gcggacgacc gcggctacgt cgcctaccca ggcgtcaatc acgccggcga gatggtcaac
     2281 ctcgtcagcg cgctgcgctc ctacgaggcg aacgtggtcg cgatgaacgc ggccaagacg
     2341 atggccttga aagcgctcga catcgggggt ggcacatgag cgcgcgtggc cgctccagag
     2401 cgctcatccc ggagcgcgca gcgcggaggg tagcccaatg aacagcatcg agcgaatcga
     2461 caactacctg ccgcctatgc ggccgacgga cgacctggtg ccatcggacc tggcgccggc
     2521 atcggccccg ccgctgcaag gcaccagcgc cttcggccag tggttcacca accaggtcga
     2581 gaaggtcaac acgcagatcg aacagtccga gcgccaactg caggccgtgg ccacaggcga
     2641 ggcgcagaac ctgcaccagg tgatgatcga tctcgaagaa gcgcggctgt cgttccagat
     2701 gctggtgcag gtgcgcaatc gcgtcctgga ggcctaccag gacgtgatgc gcaagatc
//
LOCUS       DATMJB010000628         2098 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_560840, whole genome shotgun sequence.
ACCESSION   DATMJB010000628 DATMJB010000000
VERSION     DATMJB010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560840"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VNU48_13275"
     CDS             complement(<1..619)
                     /locus_tag="VNU48_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HWI12298.1"
                     /translation="MTDSRSRPARRALFAPCLALCLAVAAMATQAADDPTNPNTFSTP
                     SYPPSQQRNAMPTGQPGQANPPGMSNSPPLRGTQIQGGYATDPRATQNANQPEFTLRR
                     TDQPPPKPGEFQKFVETATGRLLPLFGSRFFADAADAYAPVDNVPVSADYTVGPGDEI
                     ILRAWGAIDIDYRSTVDRNGLLNLPKVGSFNVSGVKASELEKHLRT"
     gene            950..1633
                     /locus_tag="VNU48_13280"
     CDS             950..1633
                     /locus_tag="VNU48_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HWI12299.1"
                     /translation="MDERPLTTVRVWDLPTRAFHWSLATCVVGSIASAWLGGNAMAWH
                     FRLGYVVFTLLAFRILWGLVGGHWSRFANFIYAPSAIARYLRGASLAHEHHEVGHNPL
                     GALSVFGLLGLLVLQVGTGLFADDEIASSGPLVKFVSGPTSLALTKWHRNFGQWLIIA
                     LVALHIGAILYYLLAKKRNLVRPMLSGDKLLAAPAPAAVDNGRSRAFAALLVALCAGL
                     VWWIVGLGD"
     gene            1638..>2098
                     /locus_tag="VNU48_13285"
     CDS             1638..>2098
                     /locus_tag="VNU48_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI12300.1"
                     /translation="MNDEPPEITLVRVTAAPWIASAREILREYAASLAVDLCFQNFEA
                     ELASLPGEYAPPAGLLLLAYVGDALAACGGLRALADVDHANACEMKRLYVRPAFRRFG
                     LGRALAEALLDEARRAGYSVMLLDTLDEMESARELYATLGFQETAPYYFNP"
BASE COUNT          265 a          683 c          791 g          359 t
ORIGIN      
        1 gcgtgcgcaa gtgtttttcg agctcggagg ccttcacgcc cgagacgttg aagctgccga
       61 ccttcggcag attcagcaag ccgttgcgat cgaccgtgct gcggtagtcg atgtcgatcg
      121 cgccccaggc gcgcaggatg atctcgtcgc cggggccgac ggtgtagtcg gccgagaccg
      181 gcacgttgtc gaccggcgcg taagcatcgg cggcgtcggc gaagaagcgg ctgccgaaca
      241 gcggcagcag ccggccggtc gcggtctcga cgaacttctg gaactcgccc ggcttgggtg
      301 gcggctggtc ggtgcgccgc agcgtgaatt cgggctggtt cgcgttctgc gtggcgcgcg
      361 ggtccgtggc gtagccgcct tgaatctgcg tcccgcgcag cggcgggctg ttcgacatgc
      421 ccggcgggtt ggcctggccc ggctggcccg ttggcatcgc gttgcgctgc tgcgagggcg
      481 ggtagcttgg cgtgctgaag gtgttggggt tggtcggatc gtcggcggcc tgcgtggcca
      541 tcgccgcgac cgcgaggcac agcgcgagac agggggcgaa cagggcgcgt cgtgcggggc
      601 gggagcggga gtctgtcatg aggtgtcggc cacgggttcg atatctggcg ccggccacgc
      661 ggcagccgat gcgtttcgct gcggcgtggc gatcgagccc ctggcgcgcg gcggcacatt
      721 gtcgaccgtc agagggtcgg cggcggagtt tagcggatgc ccgcgccacg agaacgccgc
      781 gccaacgcgc cgagcccatg cagcatccgc gagcgctggc gggtgcgcgc agccgccggc
      841 gcgcaaattc cggcctgcgc aatatccggg tgcggcgcac tggtgtggcg attgcgggta
      901 tctttcgccg cattggcgca agctctcctg ccggcgcgtc cggccgacga tggacgaacg
      961 accgttgacg accgtgcggg tctgggacct gccgacgcgg gccttccact ggtcgctggc
     1021 gacctgcgtg gtcggctcga tcgcgagtgc ctggctcggc ggcaatgcga tggcctggca
     1081 ctttcggctg ggctatgtcg tgttcacgct gctggcgttc cggatcctgt gggggctggt
     1141 cggcggacac tggtcgcgct tcgcgaactt catctacgcg ccgtcggcga tcgcacgcta
     1201 cctgcgcggc gccagcctcg cgcacgaaca ccacgaggtc ggccacaacc ccttgggtgc
     1261 gctctcggtg ttcgggttgc tcgggctgct ggtgctgcag gtcggcaccg ggctgttcgc
     1321 cgacgacgag atcgccagca gcgggccgct ggtcaagttc gtctcggggc cgaccagcct
     1381 ggcgctgacg aagtggcacc ggaacttcgg ccagtggctg atcatcgcgc tggtcgcgct
     1441 gcacatcggg gcgatcctgt actacctgct cgcgaagaag cgcaacctgg tgcggccgat
     1501 gctcagcggc gacaagctgc tcgcggcgcc cgccccggcg gccgtcgaca acggccgctc
     1561 gcgcgcgttc gcggcactgc tggtggcgct gtgcgcaggg ctggtctggt ggatcgtcgg
     1621 cctcggcgac tgaagccatg aatgacgagc cgcccgagat cacgctggta cgcgtcactg
     1681 cggcgccgtg gatcgcgtcg gcgcgcgaga tcctgcgcga gtacgccgcc agcctcgcgg
     1741 tcgatctgtg cttccagaac ttcgaggccg aactggcgag cctgccgggc gagtacgcgc
     1801 cgccggcggg cctgttgctg ttggcctatg tcggcgacgc gctcgccgcc tgcggcgggc
     1861 tgcgcgcgct ggccgatgtc gaccacgcca acgcctgcga gatgaaacgc ctgtacgtgc
     1921 ggccggcctt tcgccgcttc ggcctggggc gtgcgctggc cgaggcgctg ctcgacgagg
     1981 cgcgccgcgc cggctattcg gtgatgctgc tggacacgct ggacgagatg gagtcggcgc
     2041 gcgagctcta cgcgacactg ggcttccagg agactgcgcc gtactacttc aacccgat
//
LOCUS       DATMJB010000629         3667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_620191, whole genome shotgun sequence.
ACCESSION   DATMJB010000629 DATMJB010000000
VERSION     DATMJB010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620191"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VNU48_13290"
     CDS             complement(<1..354)
                     /locus_tag="VNU48_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWI12301.1"
                     /translation="MPGQAHVIIHRGHNGQTVFVDDRDRAGYLSSLRDAAGEAGVAIH
                     GYGLLGHELRLLATPRDLAGLARMMQSVGRRFVRSFNQRHGRTGTPWEGRFRSTAIEA
                     ETHFLACLRFVEGLSE"
     gene            complement(404..1573)
                     /locus_tag="VNU48_13295"
     CDS             complement(404..1573)
                     /locus_tag="VNU48_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006029318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HWI12302.1"
                     /translation="MATQGGLGVWASHATRSRGRRQAEPAAPTRDDFQRDRDRIVHST
                     AFRRLVYKTQVFLNHEGDLFRTRLTHSLEVAQLARSVARALRLNEDLCEAIALAHDLG
                     HTPFGHAGQDALDACLRAAHPEGGGFEHNYQSLRVVDELEERYPNFDGLNLTFEAREG
                     ILKHCSARHARALEVREPGGVGRRFLDHTQASLEAQVCNLADEIAYNTHDIDDGVRSG
                     LLAYEQLLALPAFAQFHREALAAFPGLGGRRLLFDVLRRMLSAQVYDLIGRSAARIEE
                     CAPVDADEARRSGPLVLFGAEAAESARVVKAFLRAGLYRHPRVVQTTEAARAVVRDLF
                     GAYTADPSTLPAPYATRRPNHRAVADYIAGMTDRFALREHARLFGAGRSGLADGL"
     gene            complement(1579..2673)
                     /gene="aroB"
                     /locus_tag="VNU48_13300"
     CDS             complement(1579..2673)
                     /gene="aroB"
                     /locus_tag="VNU48_13300"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HWI12303.1"
                     /translation="MHAEAPERVAIDLAERSYEILIGARILDAPQSYAGLGHGGAAVV
                     VSNTTVAPLLAERLLAALRAQYPRVLLVTLPDGEEHKTWTTLNLIFDALLQAGCDRAT
                     TLFALGGGVVGDLTGFAAACFMRGVAFVQVPTTLLAQVDSSVGGKTAVNHPLGKNMIG
                     VFHQPLRVIADLDVLDTLPEREFNAGLAEVIKYGPIADDSFLCWIEANLPALCARDKP
                     ALRHAVKRSCEIKAAIVAQDEREADLRAVLNFGHTFGHAIENGLGYGRWLHGEAVGCG
                     MVMAADLSARLGLIDAGYAQRIRDIVQRAGLPVVGPRLGAPRYLELMRVDKKARAGEI
                     RFVVIEAPGRAGVRSAPDALVTQVIEAHTA"
     gene            complement(2703..3269)
                     /locus_tag="VNU48_13305"
     CDS             complement(2703..3269)
                     /locus_tag="VNU48_13305"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HWI12304.1"
                     /translation="MILSLLGMPGGGKSTVGRHLARRLDAPFVDTDATIERRIGCSIR
                     SYFEQEGEERFRDLEAGVLSDVLEAGHSVVATGGGIVLREGNRRLLRERSICVYLHST
                     PDELFRRLRHDTKRPLLQVADPLARLRQMFAERDALYREAAHFVVETGRPSIPTLVNM
                     ILMQLELGGAIDPQDVPSPVDPPPRDER"
     gene            complement(3282..>3667)
                     /locus_tag="VNU48_13310"
     CDS             complement(3282..>3667)
                     /locus_tag="VNU48_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like group 1 domain-containing protein"
                     /protein_id="HWI12305.1"
                     /translation="PGNTFACTNEDANYNGVLDAGEDFNGSGSLDPGNVISVSTTGTS
                     SGSSSGTVKTDSTGRATISLIYAESYAPWVQVKLRAEAVVSGTESSKEATFWVTGLAS
                     DFSDQTVPPAGVDSPFGENPCNVPN"
BASE COUNT          610 a         1236 c         1252 g          569 t
ORIGIN      
        1 ctccgaaagg ccttcgacga atctgaggca ggcgaggaag tgtgtctccg cttcgatggc
       61 cgtcgagcga aatcgacctt cccagggcgt gccggttcgg ccgtggcgct ggttgaagct
      121 gcgcacgaag cgccgcccga ccgactgcat catgcgcgcg aggccggcca gatcgcgcgg
      181 cgtggccagc aggcgcagtt cgtggccgag gaggccatag ccatgaatcg ccacgccggc
      241 ttcgccggcc gcgtcgcgca ggctggacag gtagccggcc cggtcacgat cgtcgacgaa
      301 gacagtctgg ccgttgtgac cgcggtggat gatcacgtgg gcctggcccg gcatgacgat
      361 gcgtcgcaat cgcgccatga gaacgatcgc aggtggggat gaatcagagc ccgtcggcca
      421 acccgctgcg cccggcaccg aacaggcgcg cgtgctcgcg cagcgcgaag cggtcggtca
      481 tgccggcgat gtagtcggcc accgcgcggt ggttcggccg gcgcgtcgcg tagggcgcgg
      541 gcaaggtcga cggatcagcg gtgtatgcgc cgaacagatc gcgcaccacc gcgcgcgccg
      601 cttcggtcgt ctgcaccacg cgcggatggc ggtacaggcc ggcacgcagg aacgctttca
      661 cgacgcgcgc cgactcggcc gcctccgcac cgaacagcac caacggcccc gaacggcgcg
      721 cctcgtcggc atcgacagga gcgcactcct cgatgcgcgc ggcgctgcgg ccgatcaagt
      781 cgtagacctg cgccgacagc atgcggcgca gcacgtcgaa cagcagccgg cgcccgccca
      841 gcccggggaa tgcggcgagc gcttcgcgat ggaactgcgc gaaggcgggc agcgccagca
      901 gctgctcata agccagcaag ccggaccgga ccccgtcgtc gatgtcgtgc gtgttgtagg
      961 cgatctcgtc ggcgagattg cacacctgcg cctccagact ggcctgggtg tgatcgagaa
     1021 accgccggcc cacgccgcca ggctcgcgca cttccagcgc acgtgcgtgg cgcgcagagc
     1081 aatgcttgag gatgccttcg cgcgcctcga aggtcaggtt caagccatcg aagttggggt
     1141 agcgctcctc gagctcatcg acgacccgca ggctctgata gttgtgctcg aagccgccac
     1201 cctcgggatg ggccgcgcgc aggcacgcgt cgagcgcgtc ctgtccggcg tggccgaaag
     1261 gtgtgtggcc gaggtcgtgc gccagcgcga tggcctcgca gaggtcttcg ttcagtcgca
     1321 gggcgcgcgc cacggatcgc gccagctgcg cgacctcgag cgaatgcgtc aggcgcgtgc
     1381 ggaacaggtc gccctcgtgg ttcaggaaca cctgcgtctt gtagaccaga cgccggaacg
     1441 cggtgctgtg cacgatgcgg tcgcggtcgc gctgaaagtc gtcacgggtc ggtgccgcag
     1501 gctcggcttg gcgtcggcct cgggaccggg tggcatgact cgcccacacg ccgagcccgc
     1561 cctgcgtcgc cactgcggtc acgcggtgtg cgcctcgatc acctgcgtga ccagcgcgtc
     1621 cggcgcactg cgcaccccgg cccgccctgg ggcttcgatg acgacgaaac gaatctcccc
     1681 ggcgcgcgcc ttcttgtcga cccgcatcag ctcgagataa cgcggcgcgc ccagccgcgg
     1741 gccgaccacc ggcaagccgg cgcgctgcac gatgtcgcgg atgcgctgcg cgtagcccgc
     1801 gtcgatgagg ccgagccgag ccgacaggtc ggccgccatc accatcccgc agcccaccgc
     1861 ttcgccgtgc aaccagcggc cgtagcccag gccattctcg attgcatgac cgaaggtgtg
     1921 gccgaagttg agcacggcgc gcagatcggc ttcacgctcg tcctgggcca cgatcgccgc
     1981 cttgatctcg cacgaccgtt tcacggcatg gcgcagcgcc ggcttgtcac gcgcacacag
     2041 cgcgggcagg ttggcctcga tccagcagag aaaggaatcg tcggcgatcg gcccgtactt
     2101 gatgacttcg gcgaggcccg cattgaactc gcgttcgggc aaggtgtcga gcacgtcgag
     2161 gtccgcgatg acgcgcagcg gctgatggaa cacgccgatc atgttcttgc ccagcgggtg
     2221 attgacggcc gtcttgccgc ccactgacga atcgacctgc gccagcaagg tggtcggcac
     2281 ctgaacgaag gccacgccgc gcatgaagca ggccgcggca aagccggtca gatcgccgac
     2341 gacgccaccg cccagcgcga acagcgtggt cgcgcgatcg cagccggcct gcagcagcgc
     2401 atcgaagatc aggttcagcg tcgtccaggt cttgtgctct tcgccgtccg gcaacgtgac
     2461 gagcagcacg cgcgggtact gggcgcgcag cgccgccagc agccgctcgg ccaacaatgg
     2521 agcgaccgtc gtgttgctga cgacgacggc cgcgccacca tggccgaggc cggcgtagct
     2581 ctgaggcgca tcgaggatgc gcgcaccgat caggatctcg tagctgcgct ctgccagatc
     2641 gatggcgacg cgctcggggg cttctgcatg catcggccaa gtgtacgagg ccggaagagg
     2701 cctcagcgct cgtcgcgtgg cggggggtcg accggcgatg gcacatcctg cgggtcgatc
     2761 gcgcccccga gttccagttg catcaggatc atgttcacca gcgtcgggat cgacggccgg
     2821 ccggtttcga cgacgaaatg cgccgcctcg cgatacaacg catcgcgttc cgcgaacatc
     2881 tgccgcagcc gggcgagcgg atcggcgacc tgcaacagag gacgcttcgt gtcgtggcgc
     2941 aggcgccgga agagttcgtc cggtgtcgag tgaaggtaca cgcagatcga gcgctcgcga
     3001 aggagtcgac ggttgccttc gcgcagcacg atgccgccgc cggtggccac cacgctgtgg
     3061 ccggcttcaa gcacatcgga gaggacacct gcttccaggt cgcgaaagcg ctcctcgcct
     3121 tcctgttcga agtagctgcg gatcgagcag ccgatgcgcc gctcgatcgt ggcatcggtg
     3181 tcgacgaagg gcgcgtcgag acgccgtgcc agatggcggc cgaccgtgga tttgccgccg
     3241 ccgggcatgc ccaacaggct cagtatcatc caaccgggtc atcaatttgg cacgttgcaa
     3301 ggattctcgc cgaaggggct gtccacgccg gcaggcggta ctgtctggtc cgagaaatcg
     3361 gacgctaggc ctgtcaccca gaaggtggct tccttgctcg attccgttcc tgaaaccacc
     3421 gcttcggcac gaagcttgac ctgtacccac ggcgcgtagc tttcagcata gatgagcgaa
     3481 attgtcgcgc gcccagtgct gtcggtcttc acagtaccac ttgacgaccc gctggacgtc
     3541 cccgtcgtgc tcaccgagat cacgttgccc gggtcgagtg atccgctgcc gttgaagtct
     3601 tcgccggcgt ccagcacgcc gttgtaattg gcgtcctcat tggtgcaggc gaaggtattg
     3661 ccggggt
//
LOCUS       DATMJB010000630         3633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_857307, whole genome shotgun sequence.
ACCESSION   DATMJB010000630 DATMJB010000000
VERSION     DATMJB010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857307"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /gene="rlmD"
                     /locus_tag="VNU48_13315"
     CDS             complement(<1..1092)
                     /gene="rlmD"
                     /locus_tag="VNU48_13315"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HWI12306.1"
                     /translation="MTETNEWLKVESLDLEAQGVAHNQAGKVVFIEDALPGEEVQVRV
                     NRRKNNWEQATMVALRRESSQRVVPRCPHFGSCGGCKMQHFHVGAQVATKQRALEDAL
                     WHLGKVKPERVLRPIEGPDWGYRYRARLSVRFVAKKGKVLVGFHERKSSFVADMDSCE
                     VLPPHVSALLLPLRALVAAMDQRDRLPQIELAVGDTVTALVLRHLEPLSSADLARLRD
                     FGAAHGVQWWLQPKGPDTVQRLDDGGATLAYALPEFGITMPFRPTDFTQVNPHINRVL
                     VTRALRLLDARADERVIDWFCGLGNFTLPIATAAREVLGIEGAETLVQRSRENAALNG
                     LAHKTNFVARNLFEMTPVELAGFGVADKWL"
     regulatory      1124..1238
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      1247..1358
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1410..2525
                     /gene="gcvT"
                     /locus_tag="VNU48_13320"
     CDS             1410..2525
                     /gene="gcvT"
                     /locus_tag="VNU48_13320"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWI12307.1"
                     /translation="MSTSLATTPLHALHIELGAKMVPFAGYDMPVSYPAGILAEHRHC
                     RDSAALFDVSHMGQLRLIGDDAAKALESLVPMDVIDLAVGKQRYAFFTNSSGGLLDDL
                     MITRREHDLLLIVNAACKAADITHLVTHIGHRCTVQPLPDRALLALQGPKAVQALTRL
                     NTEVAALTFMTGRGFALAGAECFVTRSGYTGEDGFEISVPSDQAEALARTLLDLPEVK
                     PAGLGARDTLRLEAGLCLYGHDINEATTPVEAGLTWAIQKVRRNGGARHAGYPGAAVI
                     DAQLATGASTKRVGLVGLERVPVREGAIIVDAHGHKLGVVTSGTLSPTVNEPIAMAYL
                     AANHALPHHEVYAEVRGKRQPMRVSTMPFAPHRYFRG"
     gene            2576..2950
                     /gene="gcvH"
                     /locus_tag="VNU48_13325"
     CDS             2576..2950
                     /gene="gcvH"
                     /locus_tag="VNU48_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWI12308.1"
                     /translation="MTTMYTNDHEWINIEDHEAATVGITHHAQDALGDVVFVDLPAVG
                     STFKKGDVAGVVESVKAAADLYMPVAGEIVEVNEALRADPSLANTDPLGNGWFFKVHV
                     TNMGEFDQLMDGPAYDLLVKNS"
     gene            2977..>3633
                     /locus_tag="VNU48_13330"
     CDS             2977..>3633
                     /locus_tag="VNU48_13330"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230277.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HWI12309.1"
                     /translation="MLMSATQPLHELENASEFVARHIGIEPADERHMLSVIGAASRRA
                     LIEAIVPRGIVRAAPMLLPAPLTEAQALAELKAIASRNRVLKSFIGQGYHGTHTPGVI
                     LRNVLENPAWYTAYTPYQPEISQGRLEALINFQTMVADLTAMEIANASMLDEATAAAE
                     AMTLCRRVGRSKSRTIFVADDVFAQTADVIATRAAPLGVAVERGPAEDAASRDVFAVV
                     L"
BASE COUNT          595 a         1314 c         1209 g          515 t
ORIGIN      
        1 cagccacttg tcggcaacgc cgaagcccgc cagctcgacc ggcgtcatct cgaacaggtt
       61 gcgtgcgacg aagttcgtct tgtgcgccag gccgttgagc gcggcgttct cgcgcgagcg
      121 ttgcaccagc gtctcggcgc cttcgatgcc gagcacctcg cgcgccgcgg tggcgatcgg
      181 cagcgtgaag ttgcccaggc cgcagaacca gtcgatcacg cgctcgtcgg cgcgtgcatc
      241 gagcaggcgc agcgcgcgcg tcacgagcac gcggttgatg tgcgggttga cctgggtgaa
      301 gtcggtcggc cggaacggca tcgtgatgcc gaactcgggc agcgcatagg cgagcgtcgc
      361 accgccgtcg tccaggcgct gcaccgtatc cggccccttg ggctgcagcc accactgcac
      421 gccgtgcgcc gcgccgaagt cgcgcagccg cgccaggtcg gcggagctca gcggctcgag
      481 atgccgcagc accagcgccg tcactgtgtc gccgaccgcg agttcgatct gcggcaggcg
      541 gtcgcgctgg tccatcgcgg ccacgagtgc gcgaagcggc agcagcagcg cgctgacgtg
      601 cggtggcagg acctcgcagc tgtccatgtc ggcgacgaag ctcgacttgc gctcgtgaaa
      661 acccaccagc accttgccct tcttcgcgac aaaccgcacc gacagccgcg cccggtagcg
      721 gtagccccag tccggcccct cgatcggccg cagcacgcgc tcgggtttga ccttgccgag
      781 atgccacaac gcgtcctcga gcgcgcgctg cttggtcgcg acctgcgcgc cgacgtggaa
      841 gtgctgcatc ttgcagccgc cgcagctgcc gaagtgcggg cagcgcggca cgacacgctg
      901 cgagctctcg cggcgcagcg ccaccatcgt cgcctgttcc cagttgttct tgcgccggtt
      961 cacgcgaacc tgcacttcct cgccgggcag cgcgtcctcg atgaacacga ccttgcccgc
     1021 ctgattgtgg gccacgccct gggcttcgag atcgagcgac tcgaccttca gccattcgtt
     1081 cgtttcagtc atgggtcagg attatccggg ggcgctgcta cacttcgcgc cgtcaggaga
     1141 gagcagcccg attcctcgca gcatcgagac cgggcggccg ccgaaggcgc agagcccgaa
     1201 ccgctcgaac gctcaggcaa aaggactggc aagcgcttca tccgaagcgt tcctcgctgg
     1261 agagaggccc gccccgagcg ggtccaccga aggggcaagc gccgggctcg aaagggcacg
     1321 gcgccaatct ctcaggtaaa gcggacagag agggcaagcc ggtgcgatca tgtcgcgtcg
     1381 gtcagtgaac ttgccctcgg agaaccgcca tgtcgaccag cctcgccacg accccgctgc
     1441 acgcgctgca catcgaactc ggtgccaaga tggtgccctt cgccggctac gacatgccgg
     1501 tgtcgtaccc ggccggcatc ctggccgagc accgccactg ccgcgactcg gcggcgctgt
     1561 tcgacgtctc gcacatgggc cagctgcgcc tgatcggcga cgacgccgcg aaggcgctcg
     1621 aatcgctggt gccgatggac gtgatcgacc tcgcggtcgg caagcagcgc tacgcgttct
     1681 tcaccaacag cagcggcggg ctgctcgacg acctgatgat cacgcggcgc gagcacgacc
     1741 tgctgttgat cgtcaacgcg gcctgcaagg ccgcggacat cacccatctc gtcacccaca
     1801 tcggccaccg ctgcacggtg cagcctctgc cggatcgcgc gctgctcgcg ctgcagggcc
     1861 cgaaggcggt gcaggcactg acgcggctga acaccgaggt cgccgcgctg accttcatga
     1921 ccgggcgcgg cttcgcgctg gccggcgccg aatgcttcgt gacgcgctcg ggctacaccg
     1981 gcgaggacgg cttcgagatc tccgtgccgt ccgaccaggc cgaggccctg gcgcgcacgc
     2041 tgctcgacct gcccgaagtc aagccggccg ggctgggcgc gcgcgacacc ttgcgcctcg
     2101 aagcgggcct gtgcctgtac ggccacgaca tcaacgaagc cacgacgccg gtcgaggccg
     2161 gcctgacctg ggcgatccag aaggtgcgcc gcaacggcgg cgcccggcac gccggctacc
     2221 ccggcgcggc ggtgatcgac gcgcaactcg ccaccggcgc gagcacgaag cgggtcggcc
     2281 tggtcggtct cgaacgcgtg ccggtgcgcg agggcgcgat catcgtcgac gcgcacggcc
     2341 acaagctcgg cgtcgtgacc agcggcacgc tctcgccgac cgtcaacgag ccgatcgcga
     2401 tggcctacct ggccgcgaac cacgccttgc cgcatcacga ggtctacgcc gaggtcaggg
     2461 gcaagcgcca gccgatgcgc gtcagcacca tgccgttcgc accgcaccgt tacttccgcg
     2521 gctgaacccc gccgcgccct cttctctcac cgaccggaac cacaggagtc cccacatgac
     2581 gacgatgtac acgaacgacc acgagtggat caacatcgag gaccacgagg ccgccaccgt
     2641 cggcatcacg caccacgcgc aggacgcgct cggcgacgtg gtcttcgtcg acctgcccgc
     2701 cgtcggcagc accttcaaga agggcgacgt ggccggcgtg gtcgagtccg tcaaggccgc
     2761 ggccgacctg tacatgccgg tggccggcga gatcgtcgag gtcaacgagg cgctgcgcgc
     2821 cgacccgtcg ctggccaaca ccgacccgct gggcaacggc tggttcttca aggtccacgt
     2881 caccaacatg ggcgagttcg accagctgat ggacggcccg gcctacgacc tgctcgtcaa
     2941 gaacagctga gaagccccag ggaaagaaga tccgcgatgc tgatgtccgc cacccaaccc
     3001 ctgcacgaac tcgagaacgc ctcggagttc gtcgcccgcc acatcggcat cgagcccgcc
     3061 gacgagcgcc acatgctgtc ggtgatcggc gccgcgtcgc gccgcgcgct gatcgaggcg
     3121 atcgtgccgc gcggcatcgt gcgcgccgcg ccgatgctgc tgccggcgcc actgaccgag
     3181 gctcaggcgc tggccgagct gaaggcgatc gcgagccgca accgcgtgct gaagagcttc
     3241 atcggccagg gctaccacgg cacccacacg ccgggcgtga tcctgcgcaa cgtgctcgag
     3301 aacccggcct ggtacacggc gtacacgccc taccagcccg agatctcgca gggccggctc
     3361 gaagcgctga tcaatttcca gacgatggtg gccgatctca ccgcgatgga gatcgccaat
     3421 gcgtcgatgc tcgacgaggc gaccgccgcg gcggaggcga tgacgctgtg ccggcgcgtc
     3481 ggccgctcga agagtcgcac gatcttcgtc gccgacgacg tgttcgcgca gaccgcggac
     3541 gtgatcgcga cgcgcgccgc gccgctcggc gtcgcggtgg agcgcggtcc cgcggaggac
     3601 gcggcgtcgc gcgacgtctt cgcggtcgtg ctg
//
LOCUS       DATMJB010000631         3992 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_561478, whole genome shotgun sequence.
ACCESSION   DATMJB010000631 DATMJB010000000
VERSION     DATMJB010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561478"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VNU48_13335"
     CDS             complement(<1..291)
                     /locus_tag="VNU48_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWI12310.1"
                     /translation="MKFFIDNWFLFLAAAVSGALLLWPSIDKSAGGAGKVSTNDAVNL
                     INREKAVLIDVSEPAEYAAGHALGAKSVPLGQLETSNALPKNKALPLVVVCAT"
     gene            389..1132
                     /gene="gpmA"
                     /locus_tag="VNU48_13340"
     CDS             389..1132
                     /gene="gpmA"
                     /locus_tag="VNU48_13340"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HWI12311.1"
                     /translation="MHKLVLIRHGESTWNLENRFTGWTDVPLTPLGVEQAQQAGRLLK
                     AEGYEFDIAYTSVLKRAIWTLWHTLDQMDRTWLPVVNDWRLNERHYGALQGLNKAETA
                     KKFGDAQVLVWRRSYDTPPPVLDAADPRSERADLRYAKLAAGQVPLTECLKDTVARVL
                     PFWNDTIAPALRADRRIVIAAHGNSIRALVKYLDGISDDAIVGLNIPNGIPLVYELDA
                     ALKPIRSYYLGDAEAAAKAAAAVANQGKG"
     gene            complement(1137..1874)
                     /locus_tag="VNU48_13345"
     CDS             complement(1137..1874)
                     /locus_tag="VNU48_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWI12312.1"
                     /translation="MTRGLRAVWRLLRSVLHALHGLGIVLLRFRGLDAAQRHARIRWW
                     STKLLRLMGIAMRVEGTVHVGAVLLVANHVSWLDISALHAVVPQARFVSKADIRAWPL
                     LSRLADAADTLYLERERKRDALRVVHLVAQALSAGQTVAVFPEGTTSDGRTLLPFHAN
                     LLQAAIATGVPVQPVALRFSDARAPVSAAVEFVGATTLVQSLWRVACADALVAHLTLL
                     PPRGSRHADRRALAQTLRDDIAARLPI"
     gene            complement(1871..3172)
                     /locus_tag="VNU48_13350"
     CDS             complement(1871..3172)
                     /locus_tag="VNU48_13350"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWI12313.1"
                     /translation="MKILIKGGRVVDPASGRDEVGDLAIAAGRIVGLGQVSREFEPDR
                     VIDARGLVVAPGLVDLAARLREPGHEHEGMLESEMAAAVAGGVTSLVCPPDTEPVLDE
                     PGLVQMLKFRARNLNLAHVYPLGALTRGLKGEVLTAMAELTDAGCVGFSQAEVSLRDT
                     LVLHRALQYAATFGYTTWLRPNDAWLGNGVAAKGALATRMGLAGVPVIAETIALATIF
                     ELVRDTGARVHLCRLSSAAGIELVRRARAEGLPVSGDVSINSLHLTDVDIGYFDAAMR
                     VTPPLRQQRDRDAIRAALADGTIAALVSDHTPVDADAKHLPFAEAEPGATGLELLLSL
                     ALKWGADDGVPLQRALACVTAAPVAVVGDALGSLAASAGRLVEGGVADVCVFDPDARW
                     VVTPAALRSQGKHTPFAGYELPGRVRFTLVAGHVAHEAMPG"
     gene            complement(3169..>3992)
                     /locus_tag="VNU48_13355"
     CDS             complement(3169..>3992)
                     /locus_tag="VNU48_13355"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWI12314.1"
                     /translation="KKVPLLRGKSVFNLFFENSTRTRTTFEIAAKRLSADVINLDIAR
                     SSAAKGESLLDTIANLSAMHADMFVVRHSESGAPYLIAQHCAPHVHVVNAGDGRHQHP
                     TQGLLDMYTIRHFKRDFTRLTVAIVGDIVHSRVARSDIHALTTLGVPEIRAVGPKTLV
                     PGDLAAMGVRVCHDMAEGIRDADVIIMLRLQNERMSGAMLPSAAEFFKSYGLTPARLA
                     LAKPDAIVMHPGPINRGVEIDSEVADGTQSVILPQVTFGIAVRMAVMSIIAGNNA"
BASE COUNT          619 a         1433 c         1390 g          550 t
ORIGIN      
        1 ggtcgcgcag accacgacca gcggcaaggc cttgttcttc ggcagcgcgt tcgaggtctc
       61 gagctggccc agcggcacgc tcttcgcgcc cagcgcgtgg ccggcggcgt attccgccgg
      121 ttcgctgaca tcgatcaaca ccgccttctc gcggttgatc aggttcaccg cgtcgttcgt
      181 cgagaccttg ccggcaccac ccgcgctctt gtcgatcgac ggccacagca gcagcgcgcc
      241 ggacaccgca gcggcgagga acaggaacca gttgtcgatg aagaacttca cgggcgggcc
      301 aatcgggttg cggcaagtgg gcgattatag aattccgcct cgtcgcgcct cgccacgcgg
      361 cgcctttccc ctcacagccc cgaagcccat gcacaagctc gtactgatcc gccacggcga
      421 gtcgacctgg aacctcgaga accgcttcac cggctggacc gacgtgccgc tgacgccgct
      481 cggcgtcgag caggcccagc aggccggtcg cctgctgaag gccgagggct acgagttcga
      541 catcgcctac acctcggtgc tcaagcgcgc gatctggacc ctgtggcaca cgctcgacca
      601 gatggaccgg acctggctgc cggtcgtcaa cgactggcgc ttgaacgagc gccactacgg
      661 cgcgctgcag ggcctgaaca aggccgagac cgcgaagaag ttcggcgacg cgcaggtgct
      721 ggtctggcgc cgcagctacg acacgccgcc gccggtgctg gacgccgccg acccgcgcag
      781 cgaacgcgcc gacctgcgct acgcgaagct cgcggccggc caggtgccgc tgaccgaatg
      841 cctgaaggac acggtcgcgc gcgtgctgcc gttctggaac gacacgatcg cgccggcgct
      901 gcgcgccgac cggcgcatcg tgatcgccgc gcatggcaac agcatccgcg cgctggtgaa
      961 gtacctggac ggcatctcgg acgacgccat cgtcggcctg aacatcccga acggcatccc
     1021 gctggtctac gagctcgacg ccgcgctgaa gccgatccgc agctactacc tcggcgatgc
     1081 cgaggcggcc gcgaaggccg cggcggcggt cgccaaccag ggcaaaggct gacgggtcag
     1141 atcggcagcc gcgcggcgat gtcgtcgcgc agcgtttgcg ccagcgcgcg gcggtcggcg
     1201 tgccggctgc cacgcggcgg cagcaacgtc aggtgcgcca ccagcgcatc ggcgcaggcg
     1261 acccgccaca ggctctgaac cagggtcgtc gcgccgacga actcgaccgc tgcgctgacc
     1321 ggcgcccgcg catccgagaa gcgcagcgcg accggctgca ccggcacgcc ggtcgcgatc
     1381 gcggcctgca gcaaattggc atggaacggc agcagcgtgc ggccgtcgga cgtcgtgcct
     1441 tcgggaaaga ccgcgaccgt ctggcccgcg ctcagcgcct gcgcgaccag gtggacgacg
     1501 cgcagcgcgt cgcgcttgcg ctcgcgttcg aggtacagcg tgtcggcggc atcggccagc
     1561 cgcgacagca acggccaggc gcggatgtcg gccttggaga cgaagcgcgc ctgcggcacg
     1621 acggcgtgca gcgcgctgat gtccagccac gagacgtggt tcgcgaccag cagcaccgcg
     1681 ccgacgtgga ccgtgccctc gacgcgcatc gcgatgccca tcaggcgcag cagcttggtc
     1741 gaccaccagc ggatgcgtgc atggcgctgc gccgcatcga ggccgcgaaa gcgcagcagc
     1801 acgatgccca ggccgtgcag cgcgtgcagc acgctgcgca gcagccgcca gacggcgcgc
     1861 aggccgcgcg tcatccgggc atggcttcgt gcgcgacgtg cccggcgacg agcgtgaagc
     1921 gcacgcgacc gggcagctcg tagcccgcga acggcgtgtg cttgccctga ctgcgcagcg
     1981 cggccggggt cacgacccag cgcgcgtcgg ggtcgaacac gcagacatcg gcgacgccgc
     2041 cttcgacgag ccggccggcg ctcgcggcga gcgagccgag cgcgtcgccg accaccgcca
     2101 ccggcgcggc ggtgacgcag gccagcgcgc gctgcagcgg cacgccgtcg tcggcacccc
     2161 acttcagcgc caggctcagc agcagttcca gcccggtcgc gccgggctcg gcctcggcga
     2221 acggcaggtg cttcgcgtcg gcatcgaccg gcgtgtggtc gctgaccagc gcggcgatcg
     2281 tgccgtcggc cagcgcggcg cggatcgcgt cgcggtcgcg ctgctggcgc agcggcggcg
     2341 tgacgcgcat cgcggcgtcg aagtagccga tgtcgacatc ggtcaggtgc agcgagttga
     2401 tgctgacatc gccgctcacc ggcaggcctt cggcgcgggc gcggcgcacg agttcgatgc
     2461 ccgcggcact cgacaggcgg cacaggtgca cgcgcgcgcc ggtgtcgcgc accagctcga
     2521 agatcgtcgc cagcgcaatc gtctcggcga tcaccggcac accggccagg cccatgcgcg
     2581 tggcgagcgc gcccttcgcg gcgacaccgt tgccgagcca ggcgtcgttc gggcgcagcc
     2641 aggtcgtgta gccgaaggtc gccgcgtact gcagtgcgcg gtgcaacacc agcgtgtcgc
     2701 gcaggctgac ctcggcctgc gagaagccga cgcagccggc gtcggtgagc tcggccatcg
     2761 cggtcagcac ctcgcccttc aggccgcgtg tcaacgcgcc cagcgggtag acatgggcca
     2821 ggttcaggtt gcgggcgcgg aacttgagca tctgcaccag gccgggctcg tcgagcaccg
     2881 gctcggtgtc gggcgggcac accaggctcg tgacgccgcc ggccaccgcg gcggccatct
     2941 cggactcgag catgccctcg tgctcgtggc ccggctcgcg caggcgcgcc gcgaggtcga
     3001 cgaggccagg cgcgacgacc agcccgcgcg cgtcgatcac ccggtccggc tcgaactccc
     3061 gactcacctg gccaaggccg acgatgcgcc cggctgcgat cgcgaggtca ccgacctcgt
     3121 cgcgaccgga ggcaggatcg acgacacggc cgcccttgat gaggatcttc atgcgttgtt
     3181 ccccgcgatg atcgacatca ccgccatgcg caccgcgatg ccgaaggtca cctgcggcag
     3241 gatcacgctc tgcgtgccgt cggcgacctc ggaatcgatc tcgacgccgc ggttgatcgg
     3301 cccagggtgc atcacgatcg cgtcgggctt ggccagggcg agccgcgccg gcgtcaggcc
     3361 gtagctcttg aagaactcgg cggcgctggg cagcatcgcg ccgctcatgc gctcgttctg
     3421 caggcgcagc atgatgatca cgtcggcatc gcggatgcct tcggccatgt cgtggcagac
     3481 gcgcacgccc atcgccgcaa gatcgcccgg caccagtgtc ttcgggccga ccgcgcggat
     3541 ctcgggcacg cccagcgtcg tcagcgcgtg aatgtcggag cgcgcgacac gcgaatgcac
     3601 gatgtcgccg acgatcgcga ccgtcagccg cgtgaagtcg cgcttgaagt gcctgatcgt
     3661 gtacatgtcg agcaacccct gcgtcgggtg ctgatgtctt ccgtctcccg cattcacgac
     3721 atgcacgtgc ggcgcgcaat gctgcgcgat caggtagggc gcgccactct cgctgtggcg
     3781 cacgacgaac atgtcggcat gcatcgccga gaggttcgcg atcgtgtcga gcaggctctc
     3841 gcccttcgcg gcactcgacc gcgcgatgtc gaggttgatc acgtcggccg acaggcgctt
     3901 ggccgcgatc tcgaaggtcg tgcgggtgcg cgtgctgttc tcgaagaaca ggttgaacac
     3961 gctctttccc cgcagcagcg gcaccttctt ca
//
LOCUS       DATMJB010000632         4539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_28385, whole genome shotgun sequence.
ACCESSION   DATMJB010000632 DATMJB010000000
VERSION     DATMJB010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28385"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..479)
                     /locus_tag="VNU48_13360"
     CDS             complement(105..479)
                     /locus_tag="VNU48_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWI12315.1"
                     /translation="MAIYTTRWFDRWAHKQGLATSSLCTAVREMANGLFDADLGGGLF
                     KKRIARPGQGKSGGYRTLVATNKGNRWVFAYGFQKNERGNIDRDEEEALKKLAAHLLS
                     LTAQAVTKAKQAGELIEVDCDA"
     gene            complement(665..2821)
                     /locus_tag="VNU48_13365"
     CDS             complement(665..2821)
                     /locus_tag="VNU48_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HWI12316.1"
                     /translation="MTTARWLADLRLGLGGRRLPVILQTEAAECGLACVAMVAAHHGL
                     DSDLPTLRQRFSISLKGSTMVDLVRLAGQLQLNARALRAEMAHLPGLALPCILHWDLN
                     HFVVLKEVSRGVAVIHDPARGVRRLPLAEVSRHFTGIVLELMPQAEFKPRTERQAVGL
                     RQLLGRVSGLRRSLLQIFVLALALEFFLLLSPFFMQWVVDSVLVSLDRDLLVTLGIGF
                     ALLVLIQVATGAIRSWAVLHLSTTLNLQWLANVFAHLMRLPVAWFEKRHTGDVMSRFG
                     AVQKIQQTLTTSFIEAVLDGLLVVVTLAMMLVYSGVLTAIAVGGVAVYALLRWAFFRP
                     LREATEEAIVFEAKRASHFLESLRGVQSIKLFNRQEDRQARFMNLVVDAMNANIATRK
                     LDLMFSVGHKLVFGLERIAIVWVGALLVMDHRFSVGMLFAFIAYKEQFAMRISALIDK
                     LVDVRMLSLQGERLADIVLTAPEPQAEALVGDRELPAGLELRGVHFAYSESEPAVLRG
                     LTLRIEPGESVAIVGPSGCGKTTLLKLMLGVHAPQSGEIRVGGVVLGRLGLRAWRDMI
                     GTVMQDDQLFAGSITDNISFFDPLADPDWVAECARIAAVLDEIEAMPMGFHTLIGDMG
                     GSISGGQRQRILLARALYKRPKILFLDEATSALDVEREREVNQAIRQLQLTRIIVAHR
                     PETIASAGRVVVLHEGRVSQDLRSVPGGGSNNSHAR"
     gene            complement(2826..4085)
                     /locus_tag="VNU48_13370"
     CDS             complement(2826..4085)
                     /locus_tag="VNU48_13370"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWI12317.1"
                     /translation="MGHLFRPEAVEHRERDWLGSIQLIRPVSLAVLTCLALLTAVALA
                     TYLTFGEYTRKARVSGYLVPDRGVIRLIAPQPATVVESRVAEGRAVHRGDVLFVLAVG
                     QATPSGDTQAAVQTSLASRTLSLQSAARQRGVLEQSQLAALDRQIDEMQHELGSMKAE
                     AALQTERLALAQQAQAQYESLRNDNFVSAAQVRTKAEEVLNVKAQLQGLERQRAGRLR
                     EIAALQAQRRELPLQAQAAQGTIERDLAALAQQAAENEARRRIVVRAPQDGVVSAVLA
                     EPGQTVTPAVALASLLPADAKLQAQLFAPSSAVGFVRPDQLVELRYQAFPYQKFGHQS
                     GAVVQVSRSPLQAAELAGLALPASFNANGEPLYRITVALDRQSVAAYGAPQALAPGMQ
                     LEADVLLDRRRLIEWLFEPVLGIAGRV"
BASE COUNT          708 a         1608 c         1515 g          708 t
ORIGIN      
        1 ggcaccgttg gtcgaattcg cgcagcgtca cctggtccat cacgcccgct ttgtgcagac
       61 ccttcgccgt ctcatgcacg gcttcaagaa tcggggattt cgctttacgc atcgcagtcc
      121 acctcaatca attcgcccgc ctgcttcgcc ttcgtgaccg cctgagcagt cagcgataac
      181 aggtgagccg ccaacttctt caaagcttcc tcttcgtccc tgtcaatgtt gccgcgctcg
      241 ttcttctgga agccgtacgc gaagacccag cgattgccct tgttggtggc gaccagcgtc
      301 cgatagccac cgctctttcc ctggccaggg cgagcgatac gcttcttgaa cagaccaccg
      361 ccgaggtcgg catcgaacag cccgttcgcc atttcacgca cggcggtaca gaggcttgac
      421 gtggccagtc cctgcttgtg cgcccaacgg tcgaaccatc gggtcgtgta gatcgccata
      481 cgcccccttt ggcccattta tagcaccggg tgatactgta tgcaatacca gttttacatg
      541 tcgtgggcgt ccctaccgcg gcgtagcgcc ggcagtcggc tgctacgtgc cgcttcgcgc
      601 ccagcctggg tctcggcctg gcggtaacgc cctctgaccc gaacagcctc ggccagcggc
      661 ccccctaccg cgcgtggctg ttgttgctgc cgccgcccgg cacgctgcgc aggtcctgcg
      721 agacgcggcc ctcgtgcagc acgaccacgc ggcccgccga cgcgatcgtc tcgggccggt
      781 gcgcgacgat gatgcgcgtc agctgcagct ggcggatcgc ctggttgacc tcgcgttcgc
      841 gctccacatc gagcgcgctg gtcgcctcgt cgaggaacag gatcttcggc cgcttgtaca
      901 gcgcgcgcgc cagcaggatg cgctggcgct gcccgcccga gatgctgccg cccatgtcgc
      961 cgatcagggt gtggaaaccc atcggcatcg cctcgatctc gtcgagcacc gcggcgatgc
     1021 gcgcgcactc ggccacccag tccgggtcgg ccagcggatc gaagaagctg atgttgtcgg
     1081 tgatcgagcc ggcgaacagc tggtcgtcct gcatcacggt gccgatcatg tcgcgccagg
     1141 cgcgcagccc gagccggccg agcaccacgc cgccgacgcg gatctcgccc gactgcggcg
     1201 cgtgcacccc cagcatcagc ttcagcaggg tggtcttgcc gcagcctgaa gggccgacga
     1261 tcgcgaccga ttcgccaggc tcgatgcgca gggtcaggcc gcgcagcacc gcgggctcgg
     1321 actccgagta cgcgaagtgc acgccgcgca gctccaggcc ggccggcagt tcgcggtcgc
     1381 cgaccagcgc ctcggcctgc ggctcgggcg cggtgagcac gatgtcggcc aggcgctcgc
     1441 cctgcaggct cagcatccgc acgtcgacaa gcttgtcgat cagcgcgctg atgcgcatcg
     1501 cgaactgctc cttgtaggcg atgaacgcga acagcatgcc caccgagaag cggtggtcca
     1561 tcaccagcag cgcaccgacc cagacgatcg cgatgcgctc caggccgaac accagcttgt
     1621 ggccgacgct gaacatcagg tcgagcttgc gcgtcgcgat gttggcgttc atcgcatcga
     1681 ccaccaggtt catgaagcgc gcctggcgat cctcctggcg gttgaacagc ttgatcgact
     1741 gcacgccgcg cagcgattcg aggaagtggc tggcgcgctt ggcctcgaag acgatggcct
     1801 cctcggtcgc ctcgcgcagc ggccggaaga acgcccaacg cagcagcgca tagaccgcca
     1861 cgccgccgac cgcgatggcc gtcagcaccc cgctgtagac cagcatcatc gccagcgtga
     1921 cgacgaccag cagcccgtcg agcacggcct cgatgaagct ggtggtcagg gtctgctgga
     1981 tcttctgcac cgcgccgaag cgcgacatca cgtcgccggt gtggcgcttc tcgaaccacg
     2041 ccaccggcag ccgcatcagg tgtgcgaaca cgttggcgag ccattgcagg ttcagcgtcg
     2101 tcgacaggtg cagcacggcc cacgaacgga tcgcgccggt cgcgacctgg atcagcacca
     2161 gcagcgcgaa gccgatgccc agtgtgacca gcaggtcgcg gtcgaggctg acgagcacgc
     2221 tgtcgaccac ccattgcatg aagaacggcg acagcagcag gaagaattcg agcgccagcg
     2281 cgagcacgaa gatctgcagc agcgagcggc gcagcccgct gacccgaccg agcagctggc
     2341 gcaggccgac cgcctggcgc tcggtgcgcg gcttgaactc ggcctgcggc atcagctcga
     2401 gcacgatgcc ggtgaagtgc ctcgacacct ccgccagcgg caggcgccgc acgccgcgcg
     2461 cggggtcgtg gatcaccgcg acgccgcggc tcacttcctt cagcacgacg aagtggttca
     2521 ggtcccagtg caggatgcag ggcaaggcga gcccgggcag gtgcgccatc tcggcgcgca
     2581 gcgcgcgcgc gttgagctgc agctggccgg ccagccgcac caggtcgacc atcgtcgagc
     2641 ccttcagcga gatcgagaag cgctggcgca aggtcggcag gtcgctgtcg agcccatggt
     2701 gggccgcgac catcgcgacg caagccaggc cgcactcggc cgcttcggtc tgcaggatca
     2761 ccggcaggcg gcgcccgccc aggccgagac gcaggtcggc aagccagcgg gcggtcgtca
     2821 tggtctcaaa cccggccggc gatgccgagc accggctcga acagccactc gatcagacgg
     2881 cgccggtcga gcagcacgtc ggcctcgagc tgcatgccgg gcgccagcgc ctgcggcgcg
     2941 ccgtacgcgg cgacgctctg acgatcgagc gcgaccgtga tccggtacaa cggttcgcca
     3001 ttcgcgttga acgaggccgg cagcgccaga ccggccagct ccgcggcctg cagcggcgag
     3061 cgcgacacct gcacgaccgc acccgactgg tggccgaact tctggtacgg gaaggcctgg
     3121 tagcgcagct cgacgagctg gtccggtcgc acgaagccga ccgcgctcga cggcgcgaac
     3181 agctgcgcct gcagcttcgc gtccgccggc agcaggctgg cgagggcgac cgccggcgtc
     3241 accgtctggc ccggctcggc gagcacggcg ctgaccacgc cgtcctgcgg cgcgcgcacg
     3301 acgatgcgcc ggcgcgcttc gttctcggcg gcctgctgcg cgagcgccgc cagatcgcgc
     3361 tcgatcgtgc cctgcgcggc ctgtgcctgc aacggcagtt cgcgccgctg cgcctgcaac
     3421 gcggcgatct cgcgcagccg gccggcgcgc tggcgctcca ggccctgcag ttgcgccttg
     3481 acattgagca cctcctcggc cttcgtgcgc acctgcgccg ccgagacgaa gttgtcgttg
     3541 cgcagcgatt cgtactgcgc ctgggcctgt tgggcgagcg cgaggcgctc ggtctgcaac
     3601 gcggcctcgg ccttcatcga accgagttcg tgctgcatct cgtcgatctg gcgatcgagt
     3661 gcggcgagct ggctctgctc gagcacgccg cgctggcggg ccgcgctctg caggctgagc
     3721 gtgcgcgacg cgaggctggt ctgcaccgcc gcctgcgtgt cgccgctcgg tgtggcctgg
     3781 ccgaccgcga gcacgaacag cacatcgccg cggtgcaccg cgcgcccctc ggcgacgcgg
     3841 ctctcgacca ccgtcgccgg ctgcggcgcg atcaaccgga tcacgccgcg gtccggcacc
     3901 aggtagccgc tgacgcgcgc cttgcgcgtg tactcgccga acgtgaggta ggtcgccagc
     3961 gccaccgccg tcagcagcgc cagacacgtc aggaccgcca gcgacaccgg ccggatcagc
     4021 tgaatgctgc ccagccagtc gcgctcgcgg tgttcaacag cttcggggcg gaacagatgg
     4081 cccacggagc cggtcgcgct ttaccaggtg ccgttcggcg cggtgggtcg gggcgacgcg
     4141 gcccacgtgg cgttcggtgc caggccgcca ccgacctggc tcagctgcgc tgcgtcgagc
     4201 tcaacgggtg tcgcggcttc gatctttttt tcgtcggacg ggctcgggac acgttggtca
     4261 actgggtggt tcgtttgcat tgggggactc cggccaaagg tgggtggtga agcgcatttc
     4321 gaatgcgcag cacgattcca tcagaccggg tgcagtcccg taactgtcaa gtcttacagg
     4381 ggcgggggcg accgtcgcga cgtgcgtcgg cgtcaatcct cctagcgcaa cagacccagc
     4441 ctcagcgcct tcagcaccgc ctggtgcttg ttgacgcaac ccagcttgtg ggtcgcgttg
     4501 ttgacgtgaa gcgcggccgt gcgctcggtg atgccgaga
//
LOCUS       DATMJB010000633         6712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_725846, whole genome shotgun sequence.
ACCESSION   DATMJB010000633 DATMJB010000000
VERSION     DATMJB010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725846"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VNU48_13375"
     CDS             <1..444
                     /locus_tag="VNU48_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HWI12318.1"
                     /translation="MAAAQKLVDKVKAGDSFDLNDFLSQLSQMKKMGGLAGLMDKLPS
                     QMTGKAANLGAADVDRAERDMRRMEGIICSMTPLERRKPDLLKATRKRRVAAGAGVQV
                     QDVNRLLNQFEQMRDMMKKMKGGGMMKMMKRMGGMKGLGGPGGPR"
     gene            527..1237
                     /locus_tag="VNU48_13380"
     CDS             527..1237
                     /locus_tag="VNU48_13380"
                     /inference="COORDINATES: protein motif:HMM:NF022337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HWI12319.1"
                     /translation="MTTFQISLGLLAVVLLLAWWWLRQRGAADPGSGKTADRLDTVTG
                     WPPQPTRVLSTQERLAFGTLTRALPEYMILAQVPLARFINVPRRNSYADWLRRIGNQC
                     ADFVVCDMAAQVIAVVEVQVPQAGERAQKRLTRMQRTLKAAKVPLFLWTENAIPAADA
                     AREQILPKPEAPAVGAPAPAAAPAVAPATKPAAAGPNPFDETDRDSTQDEMIELLEPP
                     PSTWYDDLDSGPAPLKKR"
     gene            complement(1263..2438)
                     /gene="ftsY"
                     /locus_tag="VNU48_13385"
     CDS             complement(1263..2438)
                     /gene="ftsY"
                     /locus_tag="VNU48_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWI12320.1"
                     /translation="MFSFFKKKPVAPDTPPALPDPAVDSSAAPPEAERRSWLDRLRGT
                     PNEAAPPIAAPPAPEPVVAVSDAPIDTEGRRSWIDKLRGGLRKTGSSIAEVFTGTQID
                     DALYEELESALLMADAGVKATEYLLADLKWRVAEERARTPQAVKALLANAITDLLRPL
                     ERGLRVGEHVPTVIMVVGVNGAGKTTSIGKLTRHLADANQKVLLAAADTFRAAAREQL
                     AVWAQRADARTSGAGAGSRVEIVGQEGGDPAAVTFDAVLAGKARGCDVVIADTAGRLT
                     TQVHLMDELKKIRRVIAKAQDGAPHEVLLVVDGNTGQNALAQVKSFDAALQLTGLIIT
                     KLDGTAKGGVLAAIALWSRERADGGAPVVPVYFIGVGEKLEDLQTFDAREYAQALLS"
     gene            2543..2619
                     /locus_tag="VNU48_13390"
     tRNA            2543..2619
                     /locus_tag="VNU48_13390"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2577..2579,aa:Met,seq:cat)
     gene            complement(2671..3258)
                     /locus_tag="VNU48_13395"
     CDS             complement(2671..3258)
                     /locus_tag="VNU48_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HWI12321.1"
                     /translation="MKLSPRRLLRRPDRWLGFVFAASAAVCAAGVPSPANLGLSDDGA
                     YVLDVRAQVAWRRCVEGMIWNGKTCVGMPLLLNHGEAMALTRGAERRDLHLRVPRAHE
                     LQGLVHKATSPPGLDPVLFPASPRGWHWSATSSVSTVRVNQYDYGNIVQGRNEQNANH
                     LRMLHGWAMNMSTGEARDDATKRTRLPVRLVLPLD"
     gene            3451..3888
                     /locus_tag="VNU48_13400"
     CDS             3451..3888
                     /locus_tag="VNU48_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HWI12322.1"
                     /translation="MTIAWNAFSPWSALLGGVLIGIATAMFVLLNGRIAGISGVVGGL
                     LRPVDGDIAWRAAFVLGLLAAPLVYLAFAAAWPRPQIDASPGALIAAGLLVGIGTRYG
                     GGCTSGHGVCGLSRLSPRSLAATLAFMGAGFATVFAARHLSGF"
     gene            3901..4335
                     /locus_tag="VNU48_13405"
     CDS             3901..4335
                     /locus_tag="VNU48_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HWI12323.1"
                     /translation="MLVFTSLLAGLVFGLGLIVSGMANPAKVLGFLDLAGPWDPSLAF
                     VMGGAIAVGALAFALAGRRASSLIGAEMRLPAARAIDRRLIAGSVVFGIGWGIAGFCP
                     GPALVALGMGQTKALIFVGAMLAGMGLFEWLERRKPPTLAAG"
     gene            complement(4341..5201)
                     /locus_tag="VNU48_13410"
     CDS             complement(4341..5201)
                     /locus_tag="VNU48_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HWI12324.1"
                     /translation="MSKSTGQGAGRPQRGIQSIEVGGQVLLALVHQGRPMALKDLARG
                     AGMAPAKAHPYLVSFARIGLIEQDHLSGAYFLGPLALQLGLISLQQANPVQVATPLLR
                     ELAQRIGHTVALAVWGTRGATIVRLEESPAAVHVNMRHGTVFSLANTASGRLFAAFQA
                     PAAAQQQLEAERRRRKAQPEAPIAGMPPQPKVPAWKDFEKQLAEVRAHGLSRSVGEVV
                     PGVNAMAAPAFDHSGAIVLALTAIGPAGVFDTRWDGAIARALKACADQVSQRLGAPAA
                     PGRKGIRSDA"
     gene            5324..6283
                     /locus_tag="VNU48_13415"
     CDS             5324..6283
                     /locus_tag="VNU48_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWI12325.1"
                     /translation="MSQPKAFASQADLEEKKVSFTQLSEHAWAYTAEGDPNTGVVIGD
                     DAVLVADTQATPAMAADVIRRIREVTDKPIKYVVLTHYHAVRVLGASAYGAQQILASQ
                     DTFDLIVERGEQDKASEIGRFPRLFSNVETVPPGMTWPTMTFTGKMTLWLGQLEVQLL
                     QLGRGHTKGDTVVWLPQERTLLSGDLVEFDATPYAGDAYFNDWPKTLENVAALKPKAL
                     VPGRGPALVGEDRVREGLASTGGFIADVRASVQAGVAAGKDLNAVYKETFAKLEPKYG
                     HWVIFDHCMPFDVTRAYDEATGHVDPRIWTAERDIEMWHALEA"
     gene            6305..>6712
                     /locus_tag="VNU48_13420"
     CDS             6305..>6712
                     /locus_tag="VNU48_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWI12326.1"
                     /translation="MKLAELAAQPEPIDFQSLAFDYVRHPDQSADPPARHAVVVVGAG
                     PVGLALAIDLAQRGVAVVLLDNDCQLSTGSRAICFAKRTLEIFDRLGCGDAMVAKGVS
                     WNTGRVFFRDAQVYSFDLLAESGHERPAFINLQQ"
BASE COUNT         1014 a         2375 c         2309 g         1014 t
ORIGIN      
        1 atggcggcgg cccagaagct cgtcgacaag gtcaaggccg gcgacagttt cgacctgaat
       61 gacttcctgt cacaactgtc acagatgaag aagatgggcg gtctggcggg cctgatggac
      121 aagcttccgt cgcagatgac cggcaaggcg gcgaacctcg gggccgccga tgtcgaccgc
      181 gccgagcgcg acatgcgccg catggaaggc atcatctgct cgatgacgcc gctcgagcgc
      241 cgcaagcccg atctgctcaa ggccacgcgc aaacgccggg tcgccgcggg cgccggcgtg
      301 caggttcagg acgtcaatcg cctgctgaac cagttcgagc agatgcgcga catgatgaag
      361 aagatgaagg gcggcggcat gatgaagatg atgaagcgca tgggcggcat gaagggcctg
      421 ggcggccccg ggggcccgcg ctgatggcgc cttcgcgccg cgtgaccgaa cgtcgaactc
      481 gggctactct cccgccctga acgaagcgcg agcgagggac gcacggatga ctaccttcca
      541 gatttccttg ggcttgttgg ccgtcgtgct gctgctggcc tggtggtggc tgcgccagcg
      601 cggcgccgcg gatccgggtt cgggcaagac cgccgaccgc ctcgacaccg tcaccggctg
      661 gccgccgcag cccacgcgcg tgctcagcac gcaggagcgc ctggccttcg gcacgctcac
      721 gcgcgccctg cccgaataca tgatcctggc gcaggtgccg ctggcgcgct tcatcaacgt
      781 gccgcggcgc aactcatacg ccgactggct gcgccgcatt ggcaaccagt gcgccgactt
      841 cgtcgtctgc gacatggccg cgcaggtgat cgcggtggtc gaggttcagg tgccgcaagc
      901 cggcgagcgc gcgcagaagc ggctgacgcg aatgcagcgc acgctgaagg cggccaaggt
      961 gccgctcttc ctctggaccg agaacgcgat tccggccgcc gacgcggcgc gcgagcagat
     1021 cttgccgaag cccgaagccc cggcggtcgg cgcgccggca ccggccgctg cgcctgccgt
     1081 cgcgccggcg accaaacccg ccgccgccgg ccccaacccg ttcgacgaga ccgatcgcga
     1141 ctcgacccaa gacgagatga tcgagctgct ggagccgccc ccgtcgacct ggtacgacga
     1201 cctcgactcg ggaccggcac cgctgaagaa gcgctgacct tcgcccacgc cgcccgtttc
     1261 gatcagctca gcagtgcctg cgcatactcg cgcgcgtcga aggtctgcag gtcttcgagc
     1321 ttctcgccca cgccgatgaa gtagacgggc acgaccggcg cgccgccgtc cgcgcgctcc
     1381 cgcgaccaca gcgcgatcgc cgcgagcacg ccgcccttcg cggtgccgtc gagcttggtg
     1441 atgatcaggc cggtgagctg cagcgcggca tcgaagctct tcacctgcgc cagcgcgttc
     1501 tgcccggtgt tgccgtcgac caccagcagc acctcgtgcg gcgcgccgtc ctgcgccttc
     1561 gcgatgacgc gacggatctt cttcagctcg tccatcagat gaacctgcgt ggtcaggcgg
     1621 ccggcggtgt cggcgatcac cacgtcgcag ccgcgcgcct tgccggccag caccgcatcg
     1681 aaggtcaccg ccgccggatc gccgccctcc tggccgacga tctcgacccg gctgccggca
     1741 ccagcgccgg aggtgcgcgc atccgcgcgc tgggcccaga ccgccagctg ctcgcgcgcc
     1801 gcggcgcgga aggtgtcggc cgcggccagc agcaccttct gattcgcgtc ggccagatgg
     1861 cgtgtgagct tgccgatgct ggtcgtcttg ccggcgccgt tgacgccgac gaccatgatc
     1921 accgtcggca catgctcgcc cacgcgcaag ccgcgctcga gcggcctgag caggtcggtg
     1981 attgcattgg ccagcagcgc cttcacggcc tgcggggtcc gcgcccgctc ttcggcgacg
     2041 cgccacttca ggtcggcgag caggtactcg gtggccttga cgccggcgtc ggccatcagc
     2101 agcgccgatt cgagctcctc gtacagcgcg tcgtcgatct gcgtgccggt gaacacctcg
     2161 gcgatgctcg agccggtctt gcgcaagccg ccgcgcaact tgtcgatcca gctgcggcgg
     2221 ccctccgtgt cgatcggtgc gtcgctgacg gcaaccaccg gctcgggcgc cggcggcgcg
     2281 gcgatcggcg gcgccgcttc gttcggcgtg ccgcgcaggc ggtccagcca gctgcggcgc
     2341 tcggcctcgg gcggcgcagc agatgaatcg acggcggggt cggggagcgc cggcggcgtg
     2401 tcgggcgcga ccggcttttt cttgaagaag ctgaacatgc gggcgggatc gggccttgga
     2461 accagggagg gaagcgaccg cgcgggcagt gaagccgggt gtcgtcgagc gccgccggag
     2521 cgacgctata attatgggct caggcgcttt agctcagtcg gttagagcga cggaatcata
     2581 atccgcaggt ccggggttcg aatccctgaa gcgccaccaa cactaaaccc cttgtgaggc
     2641 agtgccccgc aaggggtttt tcgattgcgc tcaatcgagc ggaagcacca gacggaccgg
     2701 aagcctcgtt cgcttggtcg cgtcatcccg ggcttcgccg gtggacatgt tcatcgccca
     2761 gccgtgcagc atccggaggt ggttggcatt ctgctcattg cggccttgca cgatgttgcc
     2821 gtagtcgtac tggttgacgc gcacggtgct gaccgacgat gttgcagacc agtgccagcc
     2881 tcgcggcgag gcggggaaca gcaccggatc gaggccgggc gggctggtcg ccttgtgtac
     2941 cagcccctgc aactcgtgcg cgcgcggtac gcgcaaatgc aaatcgcgtc tctcggcgcc
     3001 gcgagtcaac gccattgctt cgccatgatt caacagcagt ggcatgccaa cgcaagtctt
     3061 gccgttccag atcattccct cgacgcaacg ccgccacgcc acctgtgcgc gcacatcaag
     3121 cacgtaggcg ccgtcatccg acaaccccag gttcgcaggg ctcggcacgc cggctgcaca
     3181 aacagcagcg gaggcggcaa agacgaagcc gagccagcgg tcggggcggc gaagaaggcg
     3241 gcgcggcgag agcttcatcg ttgacgtgat gggcgcagaa aggcgtggac ggccccaatg
     3301 agtcatggaa cccgacgtga agcatatcgg cggccgaaag cgagtggctt gattcgcatc
     3361 aagctgagcg cgccgcaagc cgcgtcagcg cgatcgaggc aagcgaggcc actaaactgg
     3421 cagcccgtcg atccgatttc ggtgcctccc atgaccatcg cctggaacgc gttcagcccc
     3481 tggtccgccc tgctgggcgg cgtcctgatc ggcatcgcga cggcgatgtt cgtgctgctc
     3541 aacggccgca tcgccggcat cagcggtgtc gtcggcgggc tgctgaggcc ggtcgacggc
     3601 gacatcgcgt ggcgcgccgc cttcgtcctc ggtttgctgg cggcaccgct cgtctacctc
     3661 gcgttcgccg ccgcgtggcc gcgcccgcag atcgacgcgt cgcccggcgc gctgatcgcc
     3721 gccgggctgc tcgtcggcat cggcacgcgc tacggcggcg gctgcaccag cggccacggc
     3781 gtctgcggtc tgtcgcgcct gtcgccgcgc tcgctggccg cgacgctggc cttcatgggt
     3841 gccggcttcg cgacggtgtt cgcggcgcgc cacctgtccg gcttctgagg agctcacacc
     3901 atgctggtct tcacgtcgct gctcgcgggc ctggtcttcg gcttggggct gatcgtctcg
     3961 ggcatggcga acccggcgaa ggtgctcggc ttcctcgacc tggccggccc gtgggatccg
     4021 tcgctggcct tcgtgatggg cggcgcgatc gcggtcggcg cgctggcgtt cgcgctggcc
     4081 ggccggcgcg ccagctcgtt gatcggcgcc gagatgaggc tgccggcggc gcgcgcgatc
     4141 gatcgccgcc tgatcgcggg aagcgtggtg ttcggcatcg gctggggcat cgccggcttc
     4201 tgcccggggc cggcgctggt cgcgctcggc atgggccaga ccaaggcgct gatcttcgtc
     4261 ggcgcgatgc tcgccggcat gggcctgttc gaatggctgg agcggcgcaa gccgccgacg
     4321 ctggccgcgg gctgagggcg tcacgcgtcc gatcggatcc ccttcctgcc cggcgccgcc
     4381 ggcgcgccga ggcgctgcga cacctggtcg gcacacgcct tcagtgcccg cgcgatcgcg
     4441 ccgtcccagc gcgtgtcgaa cacgccggcc gggccgatcg cggtcagcgc gagcacgatc
     4501 gcgccgctgt gatcgaaggc cggcgccgcc atcgcgttga cgccgggcac gacctcgccg
     4561 accgagcgcg acaggccgtg cgcgcgcacc tcggcgagct gcttctcgaa gtccttccag
     4621 gccggcacct tgggttgcgg cggcatgccg gcgatcggcg cctcgggctg cgccttgcgc
     4681 cgccggcgct cggcctcgag ctgctgctgc gccgccgccg gcgcctggaa cgcggcgaac
     4741 agccgccccg aggcggtgtt cgcgagcgag aacaccgtgc cgtggcgcat gttgacgtgc
     4801 accgccgccg gcgactcctc gagccgcacg atggtcgcgc cgcgcgtgcc ccagaccgcc
     4861 agcgccacgg tgtggccgat gcgctgcgcg agctcgcgca gcagcggcgt cgcgacctgc
     4921 accgggttcg cctgctgcag gctgatcagg ccgagctgca gcgcgagcgg ccccagaaaa
     4981 taggcgccgc tcaggtggtc ctgctcgatc aggccgatgc gcgcgaagct gaccaggtac
     5041 gggtgcgcct tcgccggcgc catgccggcc ccgcgcgcca ggtccttcag ggccatcggc
     5101 cggccctggt ggaccagcgc cagcagcacc tggccgccga cctcgatgct ctggatgccg
     5161 cgctgcgggc gcccggcgcc ctgccccgtg ctcttcgaca tggctcttcc ctcgaatcgg
     5221 ttcatcacga attcgattag acatcatcta acggatgcac tagactcaag attcgccaaa
     5281 agcgaatgac ttccgtttag acgaatctct ccaggacgcc ccgatgagcc agcccaaagc
     5341 attcgccagc caggccgacc tcgaagagaa gaaggtcagc ttcacgcaac tctccgagca
     5401 cgcctgggcc tacaccgccg agggcgaccc gaacaccggt gtcgtgatcg gcgacgacgc
     5461 ggtactggtc gccgacacgc aggccacgcc ggcgatggcc gccgacgtga tccggcgcat
     5521 ccgcgaggtc accgacaagc ccatcaagta cgtcgtgctg acccactacc acgcggtgcg
     5581 cgtgctcggc gcgtcggcct acggcgcgca gcagatcctg gccagccagg acaccttcga
     5641 tctgatcgtc gagcgcggcg agcaagacaa ggcgagcgag atcggccgct tcccgcgcct
     5701 gttcagcaac gtcgagacgg tgccgcccgg catgacctgg ccgacgatga ccttcaccgg
     5761 caagatgacc ctgtggctgg gccagctcga ggtgcagctg ctgcagctcg gccgcggcca
     5821 caccaagggc gacaccgtgg tctggctgcc gcaggagcgc acgttgctca gcggcgacct
     5881 ggtcgagttc gacgcgacgc cctacgccgg cgacgcctat ttcaacgact ggccgaagac
     5941 gctggagaac gtcgccgcgc tgaagccgaa ggcgctggta cccggccgcg gcccggcgct
     6001 ggtcggcgag gaccgcgtgc gcgaggggct ggccagcacc ggcggcttca tcgccgacgt
     6061 gcgtgccagc gtgcaggccg gcgtggcggc aggcaaggac ctgaacgcgg tctacaagga
     6121 aaccttcgcc aagctcgagc cgaagtacgg ccactgggtc atcttcgacc actgcatgcc
     6181 gttcgacgtg acccgcgcct acgacgaggc gaccggccac gtcgacccgc gcatctggac
     6241 tgccgagcgc gacatcgaga tgtggcacgc gctcgaggcc tgagcatgcc gacgcgaggc
     6301 cgtgatgaaa ctcgctgagc tggcagcgca gcccgagccg atcgacttcc agtcgctcgc
     6361 attcgactac gtgcgtcacc cggaccagtc ggccgacccg ccggcgcgcc acgcggtggt
     6421 cgtcgtcggc gccggcccgg tcggtctggc gctcgcgatc gacctggcgc agcgcggcgt
     6481 ggcggtcgtg ctgctcgaca acgactgcca gctctcgacc ggctcgcgcg cgatctgctt
     6541 cgccaagcgc acgctcgaga tcttcgaccg gctcggctgc ggcgatgcga tggtcgcaaa
     6601 gggcgtgtcg tggaacaccg gccgggtctt cttccgcgac gcgcaggtct acagcttcga
     6661 cctgctcgcc gagagcggcc acgaacgccc cgccttcatc aacctgcagc ag
//
LOCUS       DATMJB010000634         3229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_620632, whole genome shotgun sequence.
ACCESSION   DATMJB010000634 DATMJB010000000
VERSION     DATMJB010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620632"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /gene="metK"
                     /locus_tag="VNU48_13425"
     CDS             <1..1065
                     /gene="metK"
                     /locus_tag="VNU48_13425"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HWI12327.1"
                     /translation="RVAAETLCNTGLVVLAGEVTTNAHVDYIQVARDTIKRIGYDNTE
                     YGIDHKGCAVLVAYDKQSNDIAQGVDHASDDHLNTGAGDQGLMFGYACDETPDLMPAP
                     IYYAHRLVERQAQLRKDGRLPFLRPDAKSQVTMRYVDGKPHSIDTVVLSTQHSPDQSE
                     TPKKMKASFIEAVVEEIIKPVLPKGWLKETKFLINPTGRFVIGGPQGDCGLTGRKIIV
                     DTYGGACPHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLARQCQIQVAYAIGVAR
                     PMNITVYTEGTGVISDEKLSALVAEHFDLRPKGIIQMLDLLRPIYEKTAAYGHFGREE
                     PEFTWERTDKAIALRAAAGL"
     gene            1140..2231
                     /locus_tag="VNU48_13430"
     CDS             1140..2231
                     /locus_tag="VNU48_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWI12328.1"
                     /translation="MPTLNLDQLRRYAIARSLFRPTTLARAIAKLGFVQADPIRAPAR
                     AQDLTLRHRVLDYRAGDLERRYARLAIEEDFLVNYGFLPRRHHALMHPRTPRHAWTPA
                     RAKQAQAVLEFIRERRVVHPREVDAHFAHGKTTNWFGGSSNASTELLDAMHHRGLLRV
                     ARRDGGTRVYAARDGAAELPDAPTRDARMDALVDLVVAKYAPLPAATLGHLASLLCGG
                     APQWADHRAAALARAKQRLAHERVDGVAWYWPAADKPASSRWQPDARVRLLTPFDPVV
                     WDRRRFELFWGWAYRFEAYTPAPKRKLGYYALPLLWHERVIGWANAGVTDGALRCSFG
                     YTAGRAPRDAGFRAALADELERLRVFLNL"
     gene            complement(2233..2457)
                     /locus_tag="VNU48_13435"
     CDS             complement(2233..2457)
                     /locus_tag="VNU48_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12329.1"
                     /translation="MFNAARRTPSTPPATSWGALLAPGWLRRHGARNVLLAGGAGGLR
                     GGLACSTGVLLLAALIAWGQKPLVAPLDAS"
     gene            2627..>3229
                     /locus_tag="VNU48_13440"
     CDS             2627..>3229
                     /locus_tag="VNU48_13440"
                     /inference="COORDINATES: protein motif:HMM:NF044442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWI12330.1"
                     /translation="MPESTAPPKPAAPRARTFDIDCSLSYQVSGPTDFLFQIHALDGM
                     DQQVLDESLLITPDLPQHIYADPGVGHRFLRLHAETGPVELRYRARVRRRPEPVDPTA
                     PEMPIADLPDELLHNLSPTRYCESDHLSRAGQKIFGALPRGHARVQAVVDWIHANIDY
                     QVGTSLPTTTARDVFVQRVGVCRDFAHLGVTFCRALNIPAR"
BASE COUNT          505 a         1186 c         1094 g          444 t
ORIGIN      
        1 cgcgtggccg cggagacttt gtgcaacacc ggcctcgtgg tgctggccgg cgaggtcacc
       61 accaacgccc acgtcgacta catccaggtc gcgcgcgaca cgatcaagcg catcggctac
      121 gacaacaccg agtacggcat cgaccacaag ggctgcgcgg tgctggtggc ctacgacaag
      181 cagagcaacg acatcgccca gggcgtcgac cacgccagcg acgaccacct caacaccggc
      241 gccggcgacc agggcctgat gttcggctac gcctgcgacg agacgcccga cctgatgccg
      301 gcgccgatct actacgcgca tcgcctggtc gagcgccagg cgcagctgcg caaggacggc
      361 cggctgccct tcctgcgccc cgacgcgaag agccaggtga cgatgcgcta tgtcgacggc
      421 aagccgcaca gcatcgacac cgtcgtgctg agcacgcagc acagcccgga ccagagcgag
      481 acgcccaaga agatgaaggc gtccttcatc gaggccgtgg tcgaggagat catcaagccg
      541 gtgctgccga aggggtggct gaaggaaacc aagttcctga tcaacccgac cggccgcttc
      601 gtcatcggcg gcccgcaggg cgactgcggt ttgaccggcc gcaagatcat cgtcgacacc
      661 tacggcggcg cctgcccgca cggcggtggc gcgttctcgg gcaaggaccc gagcaaggtc
      721 gaccgctcgg ctgcctatgc cgcgcgctat gtcgcgaaga acatcgtcgc ggccgggctg
      781 gcgcgccagt gccagatcca ggtcgcgtac gcgatcggcg tggcgcggcc gatgaacatc
      841 acggtctaca ccgagggcac cggcgtgatc tcggacgaga agctgtcggc gctggtggcc
      901 gagcacttcg acctgcgccc gaagggcatc atccagatgc tcgacctgct gcgcccgatc
      961 tacgagaaga ccgcggccta cggccacttc gggcgcgaag agcccgagtt cacctgggag
     1021 cgcaccgaca aggcgatcgc gctgcgcgca gcggccgggc tgtaggcccg gcccgcgccg
     1081 acgatcgaga tccgccaggt cgagaccatc gacgaggctg agcttgggca tacccgggca
     1141 tgccgaccct gaacctcgac cagctgcgcc gctacgcgat cgcacgcagc ctgttccgcc
     1201 cgaccacgct ggcgcgcgcg atagccaagc tcggcttcgt gcaggccgac ccgattcgcg
     1261 ccccggcgcg cgcgcaggac ctgacgctgc gccatcgcgt gctcgactac cgcgccggcg
     1321 atctggagcg ccgctacgcg cggctcgcga tcgaagagga tttcctcgtc aactatggct
     1381 tcctgccgcg ccgccaccac gcgctgatgc acccgcgcac gccgcgccac gcgtggacgc
     1441 cggcgcgcgc gaagcaggcg caggcggtgc tcgagttcat tcgcgagcgc cgcgtcgtgc
     1501 acccgcgcga ggtcgatgcg catttcgcgc acggcaagac gacgaactgg ttcggcggtt
     1561 cgtcgaacgc gagcaccgag ctgctcgacg cgatgcacca ccgcggcctg cttcgcgtcg
     1621 cgcggcgcga cggcggcacg cgcgtctacg cggcgcgcga cggcgcggcc gaactgcccg
     1681 atgcgccgac acgagacgcg cggatggatg cgctcgtcga cctggtcgtc gccaagtacg
     1741 cgccgctgcc ggcggcaaca ctgggccacc ttgccagcct gctgtgcggc ggagcgccac
     1801 agtgggccga ccatcgtgcg gcggcgctgg cgcgcgcgaa gcagcgcctc gcgcacgagc
     1861 gcgtcgacgg cgtcgcgtgg tactggcccg ccgccgacaa gccggcctcg tcgcgctggc
     1921 agcccgatgc gcgggtgcgg ctgttgacac cgttcgaccc ggtcgtctgg gaccgccggc
     1981 gcttcgagct cttctggggc tgggcctatc gcttcgaggc ctacacgccg gcgccgaaac
     2041 gcaagctcgg ctactacgcg ctgccgctgc tgtggcacga gcgcgtgatc ggctgggcca
     2101 atgccggcgt gaccgacggc gcgctgcgct gcagctttgg ctacaccgcc ggccgcgcgc
     2161 cgcgtgacgc gggttttcgt gccgcgctgg ccgacgaact cgagcgcctg cgcgtgttcc
     2221 tgaacctctg actcaggacg catcgagggg cgcgacgagc ggcttctgac cccaggcgat
     2281 caatgcggcg agcagcagca cgccggtcga gcaggcgagg ccgccgcgca agccgcccgc
     2341 gccgcccgcc agcagcacgt tgcgcgcacc gtggcgtcgc agccagccgg gcgccagcag
     2401 cgcgccccac gaggtggccg gcggcgttga cggtgttcgt cgcgccgcgt tgaacatcgg
     2461 tgaaagtggg tcgcaccgcg gcggtacggc cggccgcagt cttgcgcacc gcgaggatgc
     2521 ttcgtcgtgc gccgttgtcc taccgatgcc tgcgtcgctt gacgatggtt cggcatggtc
     2581 ggctcgggtc aaatcgtcgc cgttgtgcat ccacaccgac cgacccatgc ccgaatccac
     2641 cgctcccccg aagcccgctg cgccccgcgc gcgcaccttc gacatcgact gctcgctgtc
     2701 gtaccaggtc agcggcccga ccgacttcct gttccagatc cacgcgctgg acggcatgga
     2761 ccagcaggtg ctcgatgaat cgctgctgat cacgccggac ctgccgcagc acatctatgc
     2821 cgacccgggc gtcggccatc gcttcctgcg cctgcacgcg gagacgggcc cggtcgagtt
     2881 gcgctaccgg gcacgggttc ggcgcaggcc cgagccggtc gacccgaccg cgcccgaaat
     2941 gccgatcgcc gacttgcccg acgaactgct gcacaacctg agcccgacgc gctattgcga
     3001 gtccgaccac ctgagccgcg ccgggcagaa gatcttcggc gcgctgccgc gcggccacgc
     3061 gcgcgtgcag gccgtcgtcg actggatcca cgcgaacatc gactaccagg tcggcacgtc
     3121 gctgccgacc accaccgcgc gcgacgtgtt cgtgcagcgc gtcggcgtct gccgcgactt
     3181 cgcgcacctg ggcgtgacct tctgccgcgc gctgaacatt ccggcgcgg
//
LOCUS       DATMJB010000635         4293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_756541, whole genome shotgun sequence.
ACCESSION   DATMJB010000635 DATMJB010000000
VERSION     DATMJB010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756541"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VNU48_13445"
     CDS             <1..728
                     /locus_tag="VNU48_13445"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWI12331.1"
                     /translation="GVDAAHKATIMSAIAFGIPVQFDKAYVEGITRLNAADIRYAEQL
                     GYRIKLLGITKRRSGAVEGIELRVHPTLVPAKQLIANVEGAMNAVMVQGDAVGSTLYY
                     GKGAGAEPTASAVIADLVDITRLHTADPDHRVPHLAFQHGALQDTRILAIDEVVTAFY
                     LRLQVADQAGVLARITAILAESDISIDALLQRESAEGEAQTDVIILTHDTVEGRMNRA
                     IAQMQALPTVLAPIVRIRKEELA"
     gene            725..2131
                     /gene="thrC"
                     /locus_tag="VNU48_13450"
     CDS             725..2131
                     /gene="thrC"
                     /locus_tag="VNU48_13450"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWI12332.1"
                     /translation="MKYLSTRGDPIQRSFCEILLEGLAPDGGLYLPAHYPKVDGATLA
                     RWRGLAYHELAFEILSLYIDDIPAADLSALTAKTYTAAVFGTTQIVPLRALEPDMYLQ
                     ALSNGPTLAFKDIAMQLLGNLFEYELARRGQVLNILGATSGDTGSAAEYAMRGKAGVN
                     VFMLSPKGRMSPFQQAQMFSLQDANIHNIAIEGVFDDCQDIVKAVSNDLAFKRRHHIG
                     TVNSINWARLLAQVVYYFAGFFQATSRDDQAVSFAVPSGNFGNICAGHVARMMGLPIR
                     QLVLATNENNVLDEFFRTGTYRVRGSAETHETSSPSMDISKASNFERFVFDLVGRDAA
                     RTRQLFKTELDLHAAFTISAAEFARIADHGFVSGSSTHADRLATIRDTHARFGTVVDT
                     HTADGLKVAREHRLPGVPTIVLETALPAKFAETIVEAIGREPDRPAAMQGIERLPKRF
                     TVMPADAAQVKSFIADHT"
     gene            2136..3371
                     /gene="glp"
                     /locus_tag="VNU48_13455"
     CDS             2136..3371
                     /gene="glp"
                     /locus_tag="VNU48_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWI12333.1"
                     /translation="MKPERAPMLTLDEALGRLLEAVSPLPASEQETLSTFDALGRVLR
                     EDVRSTLDVPPQDNTSMDGYALRCADVPVIGALVPVSQRIPAGVVGQPLQPGSAARIF
                     TGAQVPAGADAVVMQEQCEVVGEAVRINAVPCVGQWIRRRGEDVRSGDVVLPAGTRLT
                     PQALGLAATVGAATLTVTRRPRVALFSTGDELAMPGEPLKPGAIYNSNRFTLRAAIQA
                     LGCDCRDLGIVPDRLDTTRDALRRAAQGNDLIVTSGGVSVGEEDHLRPAVQAEGRVER
                     WQIAIKPGKPLAFGAVNRPDGSSAYIVGLPGNPVSSFLTLLLMVRPVLLRLQGATALT
                     PTRTPLRADFDWPRPDKRREFLRVRRNGRGGLDLFPNQSSGVLTSVVWGDGVVDNPSG
                     QAIRQGDMVEFIAFAEWLA"
     gene            3368..3619
                     /locus_tag="VNU48_13460"
     CDS             3368..3619
                     /locus_tag="VNU48_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWI12334.1"
                     /translation="MRLSVRYFASIREALGAGESVEVADGSTVGAVRDALIARGGAHA
                     RALARGRALRSALNQVLCDEAATVADGAELAFFPPVTGG"
     gene            3699..4181
                     /locus_tag="VNU48_13465"
     CDS             3699..4181
                     /locus_tag="VNU48_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HWI12335.1"
                     /translation="MPAARVTIQTADFDLGAEVAALRAGDPAVGAVASFIGTVRDRND
                     GLGVSAMELEHYPGMTERAIEAMIDEAMRRFEIRAARVIHRVGTLQPLDQIMMVAVTS
                     AHRGQAFQACEFLMDYLKTQAPFWKKEHTPEGARWVDARVSDDDALARWGLPSGNAAR
                     "
BASE COUNT          655 a         1527 c         1497 g          614 t
ORIGIN      
        1 agggcgtcga cgccgcgcac aaggcgacga tcatgagcgc aatcgcgttc ggcatcccgg
       61 tgcagttcga caaggcctac gtcgagggca tcaccaggct caacgccgcc gacatccgct
      121 atgccgagca gctcggctac cgcatcaagc tgctgggcat cacgaagcgg cgcagcggcg
      181 cggtcgaggg catcgagctg cgcgtgcacc cgacgctggt gcccgcgaag cagctgatcg
      241 cgaacgtcga gggcgcgatg aacgccgtga tggtgcaggg cgacgccgtc ggcagcacgc
      301 tgtactacgg caagggcgcc ggcgccgagc cgaccgcctc ggcggtgatc gccgacctgg
      361 tcgacatcac ccggctgcac accgccgacc ccgaccaccg cgtgccgcac ctggcgttcc
      421 agcacggcgc gctgcaggac acgcgcatcc tcgcgatcga cgaggtcgtg accgcgttct
      481 acctgcgcct gcaggtggcc gaccaggccg gcgtgctcgc gcgcatcacc gccatcctgg
      541 ccgagagcga catctcgatc gatgcgctgc tgcaacgtga gtcggccgag ggcgaggcgc
      601 agaccgacgt catcatcctg actcacgaca ccgtcgaagg ccgcatgaac cgggcgatcg
      661 cccagatgca ggcgctgccg acggtgctcg cgccgatcgt gcgcatccgc aaggaagagc
      721 tggcgtgaag tacctcagca cgcgcggcga tcccatccag cgcagcttct gcgagatcct
      781 gctcgaaggc ctcgcgcccg acggcggtct gtacctgccg gcgcactacc cgaaggtcga
      841 tggcgcgacg ctggcgcgct ggcgtggtct ggcctaccac gagctcgcgt tcgagatcct
      901 gtcgctgtac atcgacgaca ttcccgccgc cgacctgagt gcgctgaccg cgaagaccta
      961 caccgccgcc gtgttcggca cgacgcagat cgtgccgctg cgcgcgctcg agcccgacat
     1021 gtacctgcag gcgctgtcga acggcccgac gctggcgttc aaggacatcg cgatgcagct
     1081 gctgggcaac ctgttcgagt acgaactggc acgccgcggc caggtgctga acatcctcgg
     1141 cgccacctcg ggcgacaccg gcagcgccgc cgaatacgcg atgcgcggca aggccggcgt
     1201 gaacgtcttc atgctgtcgc ccaagggccg gatgagcccg ttccagcagg cgcagatgtt
     1261 cagcctgcag gatgcgaaca tccacaacat cgcgatcgag ggcgtgttcg acgactgcca
     1321 ggacatcgtc aaggcggtct cgaacgacct cgcgttcaaa cgtcgccacc acatcggcac
     1381 cgtcaactcg atcaactggg cgcggctgct ggcgcaggtg gtctactact tcgccggctt
     1441 cttccaggcc acctcacgtg acgaccaggc ggtgagcttc gcggtgccct cgggcaactt
     1501 cggcaacatc tgcgccggcc acgtcgcgcg catgatgggc ctgccgatcc gccaattggt
     1561 gctggcgaca aacgagaaca acgtgctcga cgagttcttc cgcaccggca cctaccgcgt
     1621 gcgcggcagt gccgagacgc atgagacctc gagcccgtcg atggacatct cgaaggcctc
     1681 gaacttcgag cgcttcgtgt tcgacctggt cggtcgggac gcggcgcgca cccgccagct
     1741 cttcaagacc gaactcgacc ttcatgcggc cttcacgatc agcgcggccg agttcgcgcg
     1801 catcgccgac cacggcttcg tctccggcag cagcacgcat gccgaccggc tcgcgacgat
     1861 ccgcgacacg cacgcgcgct tcggcaccgt cgtcgatacc cacaccgccg acgggctgaa
     1921 ggtcgcgcgc gagcaccggc tgccaggggt gccgaccatc gtgctcgaga ccgcattgcc
     1981 ggcgaagttc gccgagacga tcgtcgaggc gatcggccgc gagcccgacc ggcccgccgc
     2041 gatgcagggc atcgagcggc tgcccaagcg cttcacggtg atgccggccg atgcggcgca
     2101 ggtcaagtcc ttcattgcgg atcacacttg agaccatgaa gccggaacgc gcgccgatgc
     2161 tgaccttgga cgaggccctc gggcgcctgc tcgaggccgt gtcaccgctg ccggcgagcg
     2221 agcaggaaac gctgtcgacc ttcgacgcgc tcggccgcgt gctgcgtgag gacgtgcgct
     2281 cgaccctcga cgtgccgccg caggacaaca cctcgatgga cggctacgcg ctgcgctgcg
     2341 ccgacgtgcc ggtgatcggc gcgctggtgc cggtgagcca gcgcatcccc gccggcgtgg
     2401 tcggccagcc gctgcaaccc ggcagtgccg cgcgcatctt caccggcgcg caggtgccgg
     2461 ccggcgccga tgcggtcgtg atgcaggagc agtgcgaggt cgtgggcgag gcggtgcgca
     2521 tcaacgccgt gccctgcgtc ggccaatgga ttcgccggcg cggcgaggac gtgcgctcgg
     2581 gcgacgtcgt gctgcctgcc ggcacgcggc tgacgccgca ggcgctcggc ctggccgcga
     2641 ccgtcggtgc cgcgacgctg acggtcacgc gtcgcccgcg cgtcgcgctg ttctcgaccg
     2701 gcgacgagct cgcgatgccg ggcgagccgt tgaaacccgg cgcgatctac aactcgaacc
     2761 gtttcacctt gcgcgccgcg atccaggcgc tgggctgcga ctgcagggac ctcggcatcg
     2821 tgcccgaccg gctcgacacc acgcgcgacg cactgcgccg cgccgcgcag ggcaacgacc
     2881 tgatcgtcac ctcgggtggc gtctcggtcg gcgaggaaga ccacctgcgc cccgcggttc
     2941 aggccgaagg ccgagtcgag cggtggcaga tcgcgatcaa gcccggcaag ccgctggcgt
     3001 tcggcgcggt gaaccggcct gacggctcgt cggcctacat cgttgggttg ccgggcaacc
     3061 cggtgtcgag cttcctgacc ctcctgctga tggtgcgacc ggtgctgctg cggctgcagg
     3121 gcgcgaccgc gctgacgccg acccgcactc cgctgcgcgc cgacttcgac tggccgcgcc
     3181 ccgacaagcg ccgcgagttc ctgcgcgtgc ggcgcaacgg ccgcggcggg ctcgacctgt
     3241 tcccgaacca gagctccggc gtgctgacct ccgtggtgtg gggcgacggc gtggtcgaca
     3301 acccgagcgg tcaggcgatt cgccaaggtg acatggtcga gttcatcgcg ttcgcggagt
     3361 ggctggcatg aggctcagcg tgcgctattt cgcgtcgatc cgcgaggcgc tgggcgcggg
     3421 cgaatcggtc gaggtcgccg acggcagcac ggtcggcgcg gtgcgcgacg cgctgatcgc
     3481 acgcggcggc gcgcatgccc gcgccctggc ccgcggccgc gcgctgcgca gcgcgctgaa
     3541 ccaagtgctg tgcgacgagg cggcgactgt tgccgacggc gccgagctcg cgttcttccc
     3601 gccggtgacc ggaggctagg gtgggacccc caagctcact tcgttcgcag ccccccgggg
     3661 gggcgctcag tgccttgggg cggcccggcg gcactgaggt gcccgctgcg cgcgtcacga
     3721 tccagaccgc cgacttcgac ctcggcgccg aggtcgcggc cctgcgcgcg ggcgatccgg
     3781 cggtcggggc cgtggcgtcg ttcatcggca cggtgcgcga ccgcaacgac ggtctcggcg
     3841 tcagcgcgat ggagctcgag cactaccccg gcatgaccga gcgcgccatc gaagcgatga
     3901 tcgatgaggc gatgcgccgc ttcgaaattc gcgccgcgcg cgtgatccac cgcgtcggta
     3961 cgctgcagcc gctggaccag atcatgatgg tcgcggtcac ctccgcgcac cgcggccagg
     4021 ccttccaggc ctgcgagttc ctgatggact acctgaagac gcaggcgccg ttctggaaga
     4081 aggaacacac gcccgagggc gctcgctggg tcgatgcgcg ggtcagcgac gacgacgcgc
     4141 tcgcgcgctg gggcctgccg agcgggaacg ccgctcgatg acgggagcgc tgccctgggc
     4201 gcaggtgctc gcggtggcag cgggggcggc agccggcgcc ctgctgcgat gggtcagcgc
     4261 attggcgttc aaccaccccg gcggcgaggg cat
//
LOCUS       DATMJB010000636         2438 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_740051, whole genome shotgun sequence.
ACCESSION   DATMJB010000636 DATMJB010000000
VERSION     DATMJB010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_740051"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /gene="eno"
                     /locus_tag="VNU48_13470"
     CDS             <1..594
                     /gene="eno"
                     /locus_tag="VNU48_13470"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226953.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWI12336.1"
                     /translation="YAAGSQVAIGLDCAASEFYKDGKYALAGEGLTLAAADWTDILAG
                     WCDKYPIVSIEDGMAEGDWDGWKHLNERLGSKVQIVGDDLFVTNTKILKEGIEKRVAN
                     SILIKINQIGTLTETFAAIEMAKRAGWTAVISHRSGETEDSTIADIAVGTNAGQIKTG
                     SLSRSDRIAKYNQLLRIEEDLGDVASYPGRAAFYSLR"
     gene            615..893
                     /gene="ftsB"
                     /locus_tag="VNU48_13475"
     CDS             615..893
                     /gene="ftsB"
                     /locus_tag="VNU48_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HWI12337.1"
                     /translation="MRYVSLALVVLLALVHAELWFGKGGVPRMMELQYKLAEQQAANQ
                     ADRERNAQLVAEVSDLKEGLEMVEEKARFELGMVKPDEILVQLSTPKR"
     gene            932..1561
                     /gene="pnuC"
                     /locus_tag="VNU48_13480"
     CDS             932..1561
                     /gene="pnuC"
                     /locus_tag="VNU48_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HWI12338.1"
                     /translation="MLQAVQPFFAPAFTLWATPVAWLELVAFVLALVMVVLNMRVNPI
                     AWPLAIISSLLYFALFWNSRLYGDASLQIFFAIVALWGWWQWLRGTQADGSALRVRMT
                     SGRMRWWLLAALAAAWPATGLFLKTFTNTDVPWWDAFPTAASVLGQWLLGRKYVENWL
                     AWIVVNVVSVGLFAYKGLWLTTLLYSLFIAMSFVGWRAWRRLLTPAAAA"
     gene            1566..2219
                     /locus_tag="VNU48_13485"
     CDS             1566..2219
                     /locus_tag="VNU48_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI12339.1"
                     /translation="MAAERGFVIALLGAESTGKTTLALALRDALAAQARSALVVPEYL
                     REFCDRHRRTPRQDEQAGIAAEQTRRIDAAAQAHDLVIADTTALMIAVYSEQVFGDAS
                     LYPSAEAAQRACDLSLLTALDLPWQADGLQRDGPQVREPVDAKVRAALARAGTPYSVV
                     FGSGAARLAAALASVQRARAPRSGGDDASDGEVARWHWRCERCGDPNCERHGLLPRG"
     gene            complement(2232..>2438)
                     /locus_tag="VNU48_13490"
     CDS             complement(2232..>2438)
                     /locus_tag="VNU48_13490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI12340.1"
                     /translation="SGLDVLNALRADTPTEALQVVAVSANAMEVDVAKAFEHGAVDYW
                     TKPLKLDAFLAGVSARLGVPVLVS"
BASE COUNT          377 a          844 c          858 g          359 t
ORIGIN      
        1 tatgccgccg gcagccaggt cgcgatcggc ctggactgcg cggccagcga gttctacaag
       61 gacggcaagt acgccctcgc cggtgaaggc ctcacgctcg ccgccgccga ttggaccgac
      121 atcctcgcgg gctggtgcga caagtacccg atcgtctcga tcgaggacgg catggccgaa
      181 ggcgactggg acggctggaa gcacctgaac gagcgcctcg gctcgaaggt acagatcgtc
      241 ggcgacgacc tgttcgtcac caacacgaag atcctgaaag aaggcatcga gaagcgcgtc
      301 gcgaactcga tcctgatcaa gatcaaccag atcggcaccc tgaccgagac cttcgccgcg
      361 atcgagatgg ccaagcgcgc cggctggacc gcggtgatca gccaccgctc gggcgagacc
      421 gaggactcga cgatcgccga catcgcggtg ggcacgaacg ccgggcagat caagaccggc
      481 tcgctgagcc gcagcgaccg catcgccaag tacaaccagt tgctgcgcat cgaggaagac
      541 ctcggcgacg tcgcgagcta cccgggccgc gcggcgttct acagcctccg ctgagaaggc
      601 gccgccaggc cgccatgcgc tacgtctcgc tcgcgctcgt cgtgctgctc gcgctggtcc
      661 atgccgagct ctggttcggc aagggcggcg tgccgcgcat gatggagctg cagtacaagc
      721 tcgccgagca gcaggccgcg aaccaggccg accgggagcg caacgcgcag ctcgtcgccg
      781 aggtcagcga cctgaaggag ggcctggaga tggtcgaaga gaaagcccgc ttcgaactcg
      841 gcatggtcaa gcccgacgag atcctggtgc agctgtcgac gccgaagcgc tgatgcgcgc
      901 gcgcgccgtt cgctgaagga gcgtggactc gatgctgcag gccgtgcagc ccttcttcgc
      961 gccggccttc acgctctggg ccacgccggt cgcgtggctc gagctggtcg cgttcgtgct
     1021 cgcgctggtg atggtggtgc tgaacatgcg cgtcaacccg attgcctggc cgctcgcgat
     1081 catcagctcg ctgctgtact tcgcgctgtt ctggaacagc cgcctgtacg gcgacgcgag
     1141 cctgcagatc ttcttcgcga tcgtcgcgct gtggggctgg tggcagtggc tgcgcggcac
     1201 gcaggccgac ggcagtgcac tgcgcgtgcg catgacgagc ggtcgcatgc gctggtggct
     1261 gctcgccgcg ctggccgcgg cctggccggc cacggggctg ttcctgaaga ccttcacgaa
     1321 caccgacgtg ccctggtggg acgccttccc gaccgccgcg agcgtgctcg gccagtggct
     1381 gctgggccgc aagtacgtcg agaactggct cgcctggatc gtcgtcaacg tcgtcagcgt
     1441 cggcctgttc gcgtacaagg gcctgtggct gacgacgctg ctctacagcc tgttcatcgc
     1501 gatgtcgttc gtcggctggc gcgcctggcg ccgcctgctg acgccggcgg cggcggcttg
     1561 acgcgatggc agccgagcgc ggcttcgtga tcgccctgct cggtgccgag agcaccggca
     1621 agaccacgct cgcgctggca ctgcgcgacg cgctcgcggc gcaggcgcgc agcgcgctcg
     1681 tcgtgcccga atacctgcgc gagttctgcg accggcaccg gcgcacgccg cgccaggacg
     1741 aacaggcggg catcgcggcc gagcagacgc gccgcatcga cgcggccgcg caagcgcacg
     1801 acctggtgat cgccgacacc acggcgctga tgatcgcggt ctacagcgag caggtgttcg
     1861 gtgacgccag cctgtacccg tcggccgagg ccgcgcagcg cgcctgcgac ctgagcctgc
     1921 tgaccgcgct cgacctgccc tggcaggccg acggcttgca gcgcgacggg ccgcaggtgc
     1981 gcgagccggt cgacgcgaag gtgcgcgccg cgctcgcgcg cgccggcacg ccctactcgg
     2041 tggtcttcgg cagcggcgcc gctcggctcg cggcggccct ggccagcgtg cagcgcgcgc
     2101 gcgcgccgcg ttcgggcggc gacgatgcga gcgacggcga ggtcgcgcgc tggcactggc
     2161 gttgcgagcg ctgcggcgac ccgaactgcg aacgccacgg gctgctgccg cgcggctgag
     2221 gtgtgcacgg cctagctgac gaggacgggc actccaagcc gcgctgacac tccggccagg
     2281 aacgcgtcca gcttcaacgg cttggtccag tagtccacgg ccccgtgttc gaatgccttg
     2341 gccacgtcga cctccatcgc gttggccgag accgccacca cttgcaaggc ttcggtgggt
     2401 gtatcggcac gaagagcgtt gagcacatcc agaccgga
//
LOCUS       DATMJB010000637        10746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_665897, whole genome shotgun sequence.
ACCESSION   DATMJB010000637 DATMJB010000000
VERSION     DATMJB010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 10746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10746
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665897"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1941)
                     /locus_tag="VNU48_13495"
     CDS             complement(19..1941)
                     /locus_tag="VNU48_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HWI12341.1"
                     /translation="MDVISNFAARFERSREEELSIEDYLAECKRNPVAYATAAERMLK
                     AIGEPKFVDTRNDPRMSRLFANKVIKIYPAFPEFYGMEDAIEQVVSYFRHAAQGLEEK
                     KQILYLLGPVGGGKSSIAERLKQLMEQVPFYAIKGSPVNESPLGLFDVAEDGPLLEKE
                     YGIPIRYLNRIMSPWAVKRLEEFGGDIRKFKVVKRYPSVLKQVGVSKTEPGDENNQDI
                     SALVGKVDIRKLETFAQDDPDAYSFSGGLCLANQGLLEFVEMFKAPIKVLHPLLTATQ
                     EGNFKGTEGFGAIPFDGIVLAHSNESEWKTFRNNKNNEAFLDRIYIVKVPYCLRVSEE
                     VKIYEKLLRNSSLAEAKCAPGTLKMMSQFAMLTRLKEPENSSLYSKMLIYDGENLKDT
                     DPRAKSYQEYRDYAGVDEGMSGVSTRFAFKILSKVFNFDPAEIAANPVHLMYVLEQQI
                     EREQFPAELEQKYLGFIKEHLSARYAEFIGKEIQTAYLESYSEYGQNIFDRYVTYADY
                     WIQDQEYRDTDTGEVFDRGSLNAELEKIEKPAGISNPKDFRNEIVNFVLRARANNAGN
                     NPVWTSYEKLRTVIEKKMFSNTEELLPVISFNAKASADEAKKHEDFVTRMVQKGYTSK
                     QVRLLCEWYLRVRKSS"
     gene            complement(2252..3160)
                     /locus_tag="VNU48_13500"
     CDS             complement(2252..3160)
                     /locus_tag="VNU48_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWI12342.1"
                     /translation="MVTKKPKGLGLGLEALLGPRVKEPGDAPAAGNGSVPHTLKLTQL
                     RPGKYQPRTRMDEGSLYELAESIKAQGVMQPILVRPVGGEHYEIIAGERRMRAARLAG
                     LDEVPVLVRDVPDESAAAMALIENIQREDLNPLEEAQGLKRLTTEFGLTHEMAAQAVG
                     RSRSAASNLLRLLNLTEPAQQMLMAGDIDMGHARALLPLDGAQQILIATEIVAKKLSV
                     REAEKLVARTSASSGRQAPLLRAKHEKPRDLLRIEQELSDALTAAVDIRVRKRTKRGE
                     QGEVAIAFGSLDELNGLLGKLGLSQR"
     gene            complement(3154..3729)
                     /locus_tag="VNU48_13505"
     CDS             complement(3154..3729)
                     /locus_tag="VNU48_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWI12343.1"
                     /translation="MSSDALGPARLLLLPGWLGSDAAHWQSRWETLHGDERVEQDDWV
                     WPKRGDWMARLDEVLLDSERPAVLIAHSLGCQLVAAWAAHSQHVTRVRAALLVAIPDT
                     ERDDMPPNLAGWRPAVRQRLPFASLAVLSSDDPYCAPARAEQFARDWGSAAFDAGPCG
                     HINGDSGLNDWSQGRALLQTLLGRSPTIGAW"
     gene            3775..4716
                     /gene="argC"
                     /locus_tag="VNU48_13510"
     CDS             3775..4716
                     /gene="argC"
                     /locus_tag="VNU48_13510"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HWI12344.1"
                     /translation="MGYHAFVDGQEGTTGLRIHEYLAQRDDIEVLRIDADKRKDVAER
                     ARLLNAADVAFLCLPDAAAREAAALVTNPDTCLIDASTAHRTAPGWAFGLPELAPSQR
                     ALIRASKRISNPGCHASAFILLLRPLVDAGLVPAALPVTATSITGYSGGGKKMIEQYE
                     AGGDARLASPRPYALSLAHKHVPEMTIHTGLAVRPIFMPIVGNFYKGLSVSVPLHLSQ
                     LRSGATAEQVQAAFEQRYTGERFIRVMPLRDAQASTDGYFDVQACNDTNRVDIFVFAN
                     ESQLILMARLDNLGKGASGAAVQSMNVHLGLDEGKGL"
     gene            complement(4733..5518)
                     /locus_tag="VNU48_13515"
     CDS             complement(4733..5518)
                     /locus_tag="VNU48_13515"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI12345.1"
                     /translation="MPAGLNPAFDATLLSRVEDAGLNASAPPQQRWLDGWLLRFSPGK
                     AKRARCVNAVAIGRAPLNERLEACAQAFAQAELPLIVRITPFTVPADLDRRLEQRGLQ
                     RFDDARVMVLTDMRVTGSPCPAGVTIQPIGLEAFAHRVGGFRGSALAQRQAHAERLLN
                     APVPFFAFELRVDGEPAGCGQFALERDLVGLYDVFTAHAARGRGLAALLCRHLLALAR
                     SQGARHAYLQVEGDNHAARSVYHRLGFADAYAYHYRARDPSAA"
     gene            complement(5522..6307)
                     /locus_tag="VNU48_13520"
     CDS             complement(5522..6307)
                     /locus_tag="VNU48_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWI12346.1"
                     /translation="MARIFCIANQKGGVGKTTTTVNLAAGLSQIGKRVLVVDLDPQGN
                     ATMGSGVDKRALELTIYDVLLENASIAEARQLHTKAGYDVLGANRELAGAEVELVELE
                     RRNTRLKGALQAVGGEYDFVLIDCPPSLSLLTLNGLCSAHGVIVPMQCEYFALEGLSD
                     LVNTIKQVHANLNPDLEIIGLLRVMFDPRITLQQQVSEQLKSHFGDKVFDTVIPRNVR
                     LAEAPSYGLPGVVFDPASKGAIAFVEFAKEMSQRLGAAMPVEA"
     gene            complement(6406..7062)
                     /gene="rsmG"
                     /locus_tag="VNU48_13525"
     CDS             complement(6406..7062)
                     /gene="rsmG"
                     /locus_tag="VNU48_13525"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HWI12347.1"
                     /translation="MDAAIEALRPALRDVVKALALEVPQAQVDALLAYLALLQRWNAT
                     YNLTAVREPAQMLRQHLADCLAVITPLRRTLGGGPARVLDVGSGGGLPGVVVAILNPA
                     LAVTCVDAVGKKAAFVQQVAGELQLRNLRAEHRRVEQLKNEPFNLVTSRAFASLADFA
                     QLTSAQLAPRGVWMAMKGKVPADELAHLPTSVDVFHVEQVTVPGLDAERCIVWMQPKR
                     "
     gene            complement(7067..9067)
                     /gene="mnmG"
                     /locus_tag="VNU48_13530"
     CDS             complement(7067..9067)
                     /gene="mnmG"
                     /locus_tag="VNU48_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HWI12348.1"
                     /translation="MLFPKEFDVIVVGGGHAGTEAALAAARMGAATLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEIDALGGAMASATDEAGIQFRILNGSKGPAVRATRAQADRI
                     LYKAAIRRRLENQPNLWLFQGACDDLMLEGDRIAGAITQVGIRFKSRAVVLTAGTFLD
                     GKIHVGLQSHSAGRAGDPPAIALSARLKELKLPQGRLKTGTPPRLDGRSIDFSVLTEQ
                     PGDGVPRPDGTPANEPMPVFSFMGDASQHPRQLPCWITHTNERTHAIIRAGFDRSPMF
                     TGLIDGVGPRYCPSIEDKVNRFADKDSHQIFLEPEGLTTHEFYPNGISTSLPFDVQLA
                     AVRSMLGLENAHILRPGYAIEYDYFDPRELKTSFETRAVGGLFFAGQINGTTGYEEAA
                     AQGLFAGINAALQVQDREAWLPRRDQAYLGVLVDDLITKGVTEPYRMFTSRAEFRLQL
                     REDNADLRLTDAGRAMGLVDDARWAAFNRKRDAVTREMERMRSTWVHASALPAADAQR
                     LLGAPIEHDYSLAELLKRPDVGFDQVTEVAAIARPDAAVSRETLRTELGTLLADAVIE
                     QAQISIKYAGYIDKQHRDVERAVQYEALRLPDELDYSKVAALSFEARQKLGKHRPETL
                     GQASRLSGITPASISLLLVHLKKGRFKGFATLPVGTEANTAS"
     gene            complement(9146..9628)
                     /locus_tag="VNU48_13535"
     CDS             complement(9146..9628)
                     /locus_tag="VNU48_13535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWI12349.1"
                     /translation="MSVPPDPHAPAWRWCRFDELDVFELDAIYRARQQVFGIEQQCAY
                     LDADGFDAHSFHLAAWSDDRSLPLAYARVVRPGGKYAEPSIGRVITTAAARGTGLGRE
                     LVRRAIAHCVQAYPGLGIRISAQTRLERFYAEVGFAAVGAPYLEDGIPHTEMLRAPPD
                     "
     gene            complement(9660..>10746)
                     /locus_tag="VNU48_13540"
     CDS             complement(9660..>10746)
                     /locus_tag="VNU48_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI12350.1"
                     /translation="ASSVLMGPAVLLAISALVASLESSAEQREVVVAGLDRAPTLQNF
                     FERQTYTVKAAPADYEALLRKATLSDAVVVVPKDFEDALVRGDAPIVEIVSDSANQRS
                     QASAGRVAALLAGFSRERATLTLALRGVSPDAVEPVRIEERDLASTQTRATQITGMLP
                     FFVLMAVLTGALNAALDTTAGERERGSLEPLLMNPAERWVLVLGKWGAVASVGMLIAV
                     LACFSFLPGQWLLRSDRLAAMFQYGAREALLFLVVLLPFAAAASALLMAVAIRCKTFK
                     EAQASTTVVMLAASLLPLINVFSLGEESPWYLWVPALAQNVLMTHVLKGEDFSAAQLL
                     VPLFVCAVLAAGGVWFVARTLRSAAVR"
BASE COUNT         1647 a         3580 c         3630 g         1889 t
ORIGIN      
        1 cctttcagac gctgacgatc aagaactctt ccggactcgc aggtaccact cgcacaacag
       61 gcgcacctgc ttcgaggtgt agcccttctg caccatgcgc gtgacgaagt cctcgtgctt
      121 cttcgcctcg tcggcactcg ccttggcgtt gaagctgatg accggcagca gctcctcggt
      181 gttcgagaac atcttcttct cgatcaccgt gcgcagcttc tcgtagctgg tccagaccgg
      241 gttgttgccg gcgttgttgg cgcgcgcgcg cagcacgaag ttgacgatct cgttgcggaa
      301 gtccttcggg ttgctgatgc cggccggctt ctcgatcttc tccagctcgg cgttcagcga
      361 gccgcggtcg aacacctcgc cggtgtcggt gtcgcggtac tcctggtcct ggatccagta
      421 gtcggcatag gtcacgtagc ggtcgaagat gttctggccg tactcgctgt agctttcgag
      481 gtaggcggtc tggatctcct tgccgatgaa ctccgcatag cgcgccgaca ggtgctcctt
      541 gatgaagccc aggtacttct gttccagctc tgccgggaac tgctcgcgct cgatctgctg
      601 ctccagcacg tacatcagat gcaccgggtt cgcggcgatc tcggcagggt cgaagttgaa
      661 gaccttcgac aggatcttga acgcgaagcg cgtcgacacg ccgctcatgc cctcgtcgac
      721 gccggcgtag tcgcggtact cttggtagct cttcgcgcgc gggtcggtgt ctttcaggtt
      781 ctcgccgtcg tagatcagca tcttggagta cagcgacgaa ttctccggct ccttcaggcg
      841 tgtcagcatc gcgaactggc tcatcatctt cagcgtgccg ggtgcgcact tggcctcggc
      901 cagcgacgag ttgcgcagca gcttctcgta gatcttcact tcctcggaga cgcgcaggca
      961 gtacggcacc ttgacgatgt agatgcggtc gaggaaggcc tcgttgttct tgttgttgcg
     1021 gaaggtcttc cactcgctct cgttgctgtg cgcgagcacg atgccgtcga acgggatcgc
     1081 tccgaagccc tcggtgccct tgaagttgcc ctcttgcgtg gccgtcagca gcgggtgcag
     1141 caccttgatc ggcgccttga acatctcgac gaactcgagc agcccctggt tcgccaggca
     1201 caggccgccc gagaaactgt aggcgtcggg gtcgtcctgc gcgaaggtct cgagcttgcg
     1261 gatgtcgacc ttgccgacga gtgccgagat gtcctggttg ttctcgtcgc ccggctcggt
     1321 cttggacacg ccgacttgct tgagcacgct ggggtagcgc ttgacgacct tgaacttgcg
     1381 gatgtcgcca ccgaactcct cgaggcgctt cacggcccac gggctcatga tgcggttcag
     1441 gtagcggatg gggatgccgt actccttctc gagcaacggg ccgtcctcgg cgacgtcgaa
     1501 caggccgagc ggcgattcgt tcaccggcga gcccttgatc gcgtagaacg gcacctgctc
     1561 catcagctgc ttcaagcgct cggcgatcga actcttgccg ccgcccacgg gcccgagcag
     1621 atacaggatc tgcttcttct cttcgaggcc ctgcgcggca tggcggaagt acgagacgac
     1681 ctgttcgatc gcgtcttcca tgccgtagaa ctcggggaac gcggggtaga tcttgatgac
     1741 cttgttcgcg aacaggcgcg acatgcgcgg gtcgttgcgc gtgtcgacga acttgggctc
     1801 gccgatcgcc ttgagcatgc gttcggcagc ggtcgcgtag gcgacggggt tgcgcttgca
     1861 ctcggcgagg tagtcctcga tcgagagctc ttcctcgcgg ctgcgctcga agcgggcggc
     1921 aaagttgctg atcacatcca tgctgatctc ctgtggggtt accaaggaga cgctgaaggc
     1981 gggtcaggcc tcgcgttcaa cgtctgggga agttctgatg aagcccggtg gggcgccatc
     2041 agagctttcc gttttcagcg gaggggtgcg tgattcaagc ttcgttcatt ctgcgccagc
     2101 cggtccttcg agtacaaggt tcatgaccac ccatttcgat gggtgccaac aacaacatca
     2161 tcgtcgttta gcaaacaacg tgccgatgat gcggcggttg acctcgttgt tgcagagcgt
     2221 gcgtggccgc atgaagttcc gtcggcgtgc gtcagcgttg cgagagcccg agcttgccga
     2281 gcaggccgtt cagttcgtcg agcgagccga acgcgatcgc gacctcgcct tgttcgccgc
     2341 gtttggttcg cttcctgacg cggatgtcga ccgccgcggt cagcgcatcc gacagttcct
     2401 gctcgatgcg cagcaggtcg cgcggcttct cgtgtttcgc gcgcagcagc ggggcttgtc
     2461 ggcccgagct cgcgctcgtg cgtgcgacca gcttctccgc ctcgcgcacc gagagcttct
     2521 tcgcgacgat ctcggtcgcg atcaggatct gctgcgcacc gtcgagcggc agcaatgcac
     2581 gtgcatggcc catgtcgatg tcgccggcca tcagcatctg ctgcgccggc tcggtcaggt
     2641 tgagcaggcg cagcaggttg ctcgcggcgc tgcgtgagcg gcccaccgcc tgtgcggcca
     2701 tctcgtgcgt caggccgaac tcagtggtca gccgcttcag gccctgcgct tcttcgagcg
     2761 ggttcaggtc ttcgcgctgg atgttctcga tcagcgccat cgcggcggcg gactcgtcgg
     2821 gcacgtccct gaccagcacc ggcacctcgt ccagaccggc gagcctggcg gcacgcatgc
     2881 ggcgctcgcc cgcgatgatc tcgtagtgct cgccgccgac cggccgcacc aggatcggct
     2941 gcatcacgcc ttgcgccttg atgctctcgg ccagctcgta gagcgagcct tcgtccatgc
     3001 gcgtgcgcgg ttggtacttg cccggtcgca gctgcgtcag tttgagcgtg tgggggacgc
     3061 tgccgttgcc ggcggcgggc gcgtcgcccg gttccttcac gcgcgggccg agcagcgctt
     3121 cgaggccgag gccgaggccc ttgggtttct tcgtcaccat gcgccgattg tcggtgatcg
     3181 gccgagcagg gtttgcagca gcgcccgccc ctgcgaccag tcgttcaggc ccgagtcgcc
     3241 attgatgtgg ccgcacgggc cagcgtcgaa ggccgcgctg ccccaatcgc gcgcgaattg
     3301 ctcggcacgg gccggcgcgc agtacggatc gtcgctgctg agcacggcaa ggctcgcaaa
     3361 gggcaggcgt tggcgcacgg ccggtcgcca tccggcaagg ttcggcggca tgtcgtcgcg
     3421 ctcggtatcg gggatcgcca ccagcagcgc ggcgcgcacg cgcgtcacgt gctgcgagtg
     3481 ggccgcccag gcggcgacga gctggcagcc cagactgtgc gcgatcagca ccgcagggcg
     3541 ctcggagtcg agcagcacct cgtccagacg cgccatccag tcgccacgct tgggccacac
     3601 ccagtcgtcc tgttccacgc gctcgtcgcc gtgcagcgtt tcccagcggc tctgccagtg
     3661 agcggcatcc gagccgagcc agcccggcaa cagcagcaag cgcgcggggc cgagggcgtc
     3721 actggacatg ggactggttt atccttgcaa gctgtttcga cgagagggct ggcgatgggc
     3781 taccacgcat ttgtcgatgg ccaggaaggc accaccggcc tgcgcatcca tgagtatctc
     3841 gcgcagcgtg acgacatcga ggtgctgcgc atcgatgccg acaaacgcaa ggacgtggcc
     3901 gagcgcgcgc gcctgctgaa cgctgccgat gtcgcgttcc tgtgcctgcc cgacgcggcg
     3961 gcgcgcgagg ccgccgcgct cgtcacgaac cccgacacct gcctgatcga cgcgagcacc
     4021 gcgcaccgca cggcgccggg ctgggccttc ggcttgccgg aactggcgcc atcgcagcgc
     4081 gcgctgatcc gcgcgtccaa gcgcatctcg aatccgggct gccatgcgtc ggcgttcatc
     4141 ctgctgctgc gtccgctggt cgatgcgggc ctggtcccgg cggcgctgcc ggtcacggcc
     4201 acgtcgatca ccggttattc gggcggcggc aagaagatga tcgagcagta cgaggctggc
     4261 ggcgatgcgc gcctggcctc gccacgcccc tatgcgctga gccttgcgca caagcatgtg
     4321 ccggagatga cgatccacac gggtctcgcg gtgcggccga tattcatgcc catcgtcggc
     4381 aatttctata aggggttgtc tgtaagcgtg cccttacacc tatcgcagct caggagcggt
     4441 gcgaccgcgg aacaggtgca ggcggcgttc gagcagcgct atacgggtga gcgctttatt
     4501 cgcgtcatgc cattgcgcga tgcgcaagcg tcgacggacg gctacttcga cgttcaggcc
     4561 tgcaacgaca ccaatcgtgt cgacatcttc gtcttcgcga acgaatcgca gctgatcctg
     4621 atggcgcggc tcgacaacct cggcaagggc gccagcgggg ccgcggtgca gtcgatgaac
     4681 gtgcacctcg gtttggacga gggcaaaggc ctttgaccga ttgcggcgtg gctcaggctg
     4741 cggacggatc ccgggcgcgg tagtgatagg cgtaggcatc ggcgaacccg agtcggtggt
     4801 agaccgagcg cgcggcgtgg ttgtcgccct cgacctgcag gtacgcgtgc cgtgcccctt
     4861 gcgagcgcgc cagcgcgagc aggtgacggc agagcagggc cgcgagcccg cgaccccgcg
     4921 cggcgtgagc ggtgaacacg tcgtagaggc cgacgagatc ccgttcgagg gcgaattgcc
     4981 cgcagccggc aggctcgccg tcgacgcgta gctcgaaggc gaagaacggc acaggtgcgt
     5041 tcagcaggcg ttcggcgtgc gcttggcgct gagcgagcgc tgaacctctg aagccaccaa
     5101 cgcggtgcgc gaaggcctcg aggccgatcg gctggatcgt gacgcctgcc gggcacggtg
     5161 agccggtgac gcgcatgtcg gtcagcacca tcacgcgggc gtcgtcgaag cgctgcaggc
     5221 cccgctgttc gaggcgccga tcgaggtcgg ccggcacggt gaagggcgtg atccgcacga
     5281 tcaacggcaa ctcggcctgc gcgaatgcct gcgcgcaggc ctcgagtcgt tcgttcagcg
     5341 gcgctcgccc gatcgccacc gcattgacgc aacgcgcgcg cttggccttg cccggtgaga
     5401 agcgcagcag ccagccatcg agccaacgtt gctggggcgg ggcactcgcg ttcagcccgg
     5461 cgtcttcgac acgcgacagc agcgtcgcgt cgaatgccgg gttcagccca gccggcatgg
     5521 ctcaggcctc gaccggcatg gcagccccca gtcgctgcga catttccttc gcgaactcga
     5581 cgaaggcgat cgctcccttc gatgcggggt cgaacacgac gcccggcagg ccgtagctcg
     5641 gcgcctcggc gagccggaca ttgcgtggga tcaccgtgtc gaacaccttg tcgccgaagt
     5701 gcgacttcag ctgctcgctg acctgctgct gcagggtgat gcgcgggtcg aacatcacgc
     5761 gcagcaggcc gatgatctcc aggtcggggt tcaggttggc gtgcacctgc ttgatcgtgt
     5821 tgaccaggtc gctcagccct tcgagcgcga aatactcgca ctgcatcggc acgatgaccc
     5881 cgtgagcgct gcacaggccg ttgagcgtca gcaggctcag cgacggcggg cagtcgatca
     5941 gcacgaagtc gtactcgccg cccacggcct gcagcgcgcc cttcagccgg gtgttgcgcc
     6001 gctcgagttc gaccagctcg acttcggcgc cggcgagctc gcggttcgca ccgagcacgt
     6061 cgtacccggc cttggtgtgc agctggcggg cttcggcgat cgaggcgttt tcgagcagca
     6121 cgtcgtagat cgtgagttcg agtgcccgct tgtcgacgcc ggagcccatc gtcgcattgc
     6181 cttgcgggtc gaggtcgacg acgagtacac gcttgccgat ctggctcagg ccggcggcca
     6241 ggttcacggt ggtggtggtc ttgccgacgc cgcccttctg gttggcaatg cagaaaattc
     6301 tggccatggg cgctcgacgg gttgggagag ggcaatcagc gcatccggtg acggtcgcgc
     6361 tgcgctcgaa ttatagggag cggcctcgtc ccaccgtccg gctgttcagc gtttcggctg
     6421 catccagacg atgcagcgct cggcgtccag ccccggaacg gtgacttgtt ccacgtgaaa
     6481 cacgtcgacg ctggtcggca agtgcgccag ctcatcggcc ggcaccttgc ccttcatcgc
     6541 catccagacg ccgcgcgggg ccagctgcgc gctggtcagt tgagcgaaat cggccagcga
     6601 agcgaacgcg cgcgatgtga cgaggttgaa tggctcgttc ttcagctgct ctacacggcg
     6661 atgctcggcg cgcaggttgc gcaactgcag ctcgcccgcg acctgttgga cgaatgccgc
     6721 cttcttgccg acggcatcga cgcaggtgac cgcgagcgcg ggattcagaa tcgccaccac
     6781 cacgcccggc aagccgccgc cgctgccgac gtcgagcacg cgggccgggc cgccgcccag
     6841 cgtccggcgc agcggcgtga tgaccgccag gcagtcggcc aggtgctgcc tcagcatctg
     6901 cgcgggctcg cgcacggccg tcaggttgta cgtggcgttc cagcgctgga gcagcgcgag
     6961 gtaggcgagc agggcgtcga cctgcgcctg cggcacctcc agcgccaggg ccttgacgac
     7021 gtcgcgcagc gccgggcgca aggcctcgat cgcggcatcc atgacttcag gacgccgtgt
     7081 tggcttccgt gcccacgggc agcgtcgcga accccttgaa ccgacctttc ttcaggtgta
     7141 ccagcagcag cgaaatcgat gccggcgtga tgcccgacag gcgcgatgcc tgacccaatg
     7201 tctcgggccg gtgcttgccg agcttctggc gtgcctcgaa cgacagcgcc gccaccttgg
     7261 aatagtcgag ttcgtcgggc aggcgcaacg cctcgtactg cacggcgcgt tcgacgtcgc
     7321 gatgctgctt gtcgatgtag ccggcgtact tgatgctgat ctgggcctgc tcgatcaccg
     7381 cgtcggccag cagcgttccg agttcggtgc gcaaggtttc acgtgaaaca gcggcgtcgg
     7441 gccgcgcgat cgcggcgact tccgtgacct gatcgaaccc gacgtccggt cgcttcagca
     7501 attcggccag gctgtagtcg tgctcgatcg gtgcgccgag caggcgttgc gcgtcggcgg
     7561 cgggcagcgc gctcgcgtgc acccaggtcg acctcatgcg ctccatctca cgcgtcacgg
     7621 cgtcgcgctt gcggttgaag gccgcccagc gcgcgtcgtc gaccagtccc atcgcgcggc
     7681 cggcatcggt caaacgcagg tcggcgttgt cttcgcgcag ctgcaggcgg aactcggcgc
     7741 ggctcgtgaa catgcggtac ggctcggtga cgcccttggt gatcaggtcg tcgaccagca
     7801 cgccgaggta ggcctgatcg cggcgtggca gccaggcctc gcggtcctgc acctgcagcg
     7861 cggcgttgat gccggcgaac aggccctgcg cggcggcctc ttcatagccg gtcgtgccgt
     7921 tgatctgacc ggcgaagaac agcccgccga cggctcgcgt ctcgaaactc gtcttcaact
     7981 cgcgcgggtc gaagtagtcg tattcgatcg cgtagccggg gcgcaggatg tgcgcgttct
     8041 ccagcccgag catgctgcgc actgccgcca gttgcacgtc gaagggcagc gaggtcgaga
     8101 tgccgttcgg gtagaactcg tgggtcgtca gcccctcggg ctcgagaaag atctggtgcg
     8161 agtccttgtc cgcgaagcgg ttcaccttgt cctcgatgct cgggcagtag cgcgggccga
     8221 caccgtctat caggccggtg aacatcgggc tgcggtcgaa gccggcgcgg atgatcgcgt
     8281 gggtgcgctc gttcgtgtgc gtgatccagc acggcagctg gcgcgggtgc tgcgacgcgt
     8341 cgcccatgaa gctgaacacc ggcatcggct cgttggcggg cgtgccgtcg ggtcgcggca
     8401 cgccgtcgcc cggttgctcg gtgagcacgc tgaaatcgat cgagcgccca tccagacgcg
     8461 gtggcgtgcc cgtcttcagg cgcccctgcg gcagcttcag ctccttcagg cgcgccgaca
     8521 gcgcaatcgc cggcgggtcg ccggcgcgtc ccgcgctgtg gctttgcagc ccgacgtgga
     8581 tcttgccgtc gaggaaggtg cccgcggtca gcaccacggc gcgcgatttg aagcgaatgc
     8641 ccacttgcgt gatcgccccc gcaatcctgt caccttccag catcaggtcg tcgcacgcac
     8701 cttggaatag ccacaggttc ggctgattct ccaggcgccg gcggatggcc gccttgtaga
     8761 gaatgcggtc ggcctgcgct cgcgttgcgc gcaccgccgg gcccttcgag ccgttcagga
     8821 tgcggaactg gatgccggcc tcgtcggtgg ccgatgccat ggcgccgccc agcgcatcga
     8881 tctccttgac caggtggccc ttgccgatcc cgccgatcga cgggttgcag ctcatctggc
     8941 ccagcgtctc gatgttgtgc gtgagcagca aggtggcggc gcccatgcgc gccgcggcca
     9001 gcgccgcctc ggtgccggca tggccgccgc cgaccacgat cacgtcgaat tccttcggga
     9061 acagcatcgc gttgcctgat ttttaagcaa agcgggattg tagggttgag gccgccggca
     9121 cgtcagccca aggggcgagc gggattcagt cgggcggtgc tcgcagcatc tcggtgtgcg
     9181 ggatgccgtc ttcgaggtat ggcgcgccga cggcggcgaa gccgacttcg gcatagaagc
     9241 gctccagccg cgtctgcgcc gagatgcgga tgcccaggcc cggataggcc tgaacgcagt
     9301 gcgcgatcgc gcgccggacc agctcgcgac cgagcccggt gccgcgcgcc gcggcggtcg
     9361 tgatgacgcg gccgatcgat ggttcggcgt acttgccgcc gggccgcacg acacgcgcat
     9421 aggcgagcgg caacgaacgg tcgtccgacc acgccgcgag gtggaacgaa tgcgcgtcga
     9481 agccgtcggc gtcgaggtag gcacactgct gctcgatccc gaatacctgc tggcgtgcac
     9541 ggtagatcgc atccagttcg aacacgtcga gctcgtcgaa tcggcaccag cgccaggccg
     9601 gtgcatgcgg gtcgggcggg acgctcatcg tgacatcgtc gccgattcgc cgcagaagtt
     9661 cagcgcaccg ccgcgctgcg cagcgtgcgc gccacgaacc agacgccgcc ggcggccagc
     9721 accgcgcaga cgaacaaggg cacgagcagc tgcgccgcgc tgaagtcctc gcccttcagc
     9781 acatgcgtca tcaacacgtt ctgcgccagc gccggcaccc acaggtacca cggcgactcc
     9841 tcgcccaggc tgaagacgtt gatcagcggc agcagcgaag ccgcgagcat cacgaccgtc
     9901 gtgctcgcct gtgcctcctt gaaggtcttg cagcggatcg ccaccgccat cagcagcgcc
     9961 gaggcggctg ccgcgaacgg cagcagcacg accaggaaca gcagcgcctc gcgcgcgccg
    10021 tactggaaca tcgccgcgag ccggtcgctg cgcaacagcc attggccggg caggaagctg
    10081 aagcacgcga gcaccgcgat cagcatgccc acgctcgcca ccgcgcccca cttgccgagc
    10141 accagcaccc agcgctcggc cgggttcatc agcagcggtt caagcgagcc gcgctcgcgc
    10201 tcgccggcgg tcgtgtccag tgccgcgttc agcgcgccgg tcagcacggc catcaacacg
    10261 aagaagggca gcatgccggt gatctgggtt gcgcgcgtct gcgtgctggc aaggtcgcgc
    10321 tcttcgatgc gcaccggctc aaccgcatcg ggcgagacgc cgcgcagcgc cagtgtcagc
    10381 gtcgcgcgct cgcggctgaa gccggcgagc agcgccgcga cccgaccggc gctggcctgc
    10441 gagcgctggt tcgcgctgtc ggagacgatc tcgacgatcg gcgcgtcgcc gcgcaccagc
    10501 gcgtcctcga aatccttggg cacgacgacg accgcgtcgc tcagcgtcgc cttgcgcagc
    10561 agcgcttcgt agtcggccgg cgcggccttc acggtgtagg tctggcgctc gaagaagttc
    10621 tgcagcgtcg gcgcacggtc gagaccggcg accacgacct cgcgctgctc ggcgctcgat
    10681 tcgagcgagg ccaccagcgc cgagatcgcg agcagcaccg ccggccccat cagcaccgac
    10741 gaggcg
//
LOCUS       DATMJB010000638         2582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_502937, whole genome shotgun sequence.
ACCESSION   DATMJB010000638 DATMJB010000000
VERSION     DATMJB010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502937"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..877)
                     /locus_tag="VNU48_13545"
     CDS             complement(17..877)
                     /locus_tag="VNU48_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HWI12351.1"
                     /translation="MREGLVPAGVGLRQPHYTALLDSVPPLGFVEVHSENFFADGGAA
                     LAVLRRAREHYAVSLHGVGLALGSAVGLDPWHLERLALLARAIEPVRVSDHASFARAP
                     RVPDGPIVHANDLLPIAFTEAALAVMVANVSQVQERLGRTLLVENLSAYVSFADDSMS
                     EPEFFTQLCRRSGCGMLLDVNNLMVNALNAGVADPLAACCGFVDALPLGIVGEVHLAG
                     YADADGLAIDDHGSRVRPAVWSVYEHALRRFGAAVPTLVEWDTDVPALDVLLGEAARA
                     TTIAARVAAA"
     gene            complement(877..1218)
                     /locus_tag="VNU48_13550"
     CDS             complement(877..1218)
                     /locus_tag="VNU48_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HWI12352.1"
                     /translation="MAAPAGASTRSRSTTGDSDTMNARLIASSALASVLAMGALSAAH
                     AAEGKEKEKCFGIAKEGTNDCANLSGSHSCAGQNKNAMAPDEWNYVAKGSCAKIGGIS
                     EDEAKALIAKK"
     gene            complement(1263..>2582)
                     /locus_tag="VNU48_13555"
     CDS             complement(1263..>2582)
                     /locus_tag="VNU48_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HWI12353.1"
                     /translation="PRGSPGPASERPGAGPAGDDEGRILFYVGSRGHHADIGGVTPGS
                     MPPFSTRIDEEGVQIDNVKLVDRGTLREAEMLALLGSGEFPSRNPQQNLADLKAQIAA
                     NEKGVQELRRMVRQFGLDVVQAYMRHVQDNAEESVRRVITKLKDGAFTLPLDNGAQIS
                     VAIRVDAAARSAEIDFTGSSAQLANNFNAPTAVCMAAVLYVFRTLVNDDIPLNAGCLK
                     PLRVIIPDGSMLNPHPPASVVAGNVETSTCITNALYGALGVMAAGQCTMNNFTFGNAR
                     HQYYETISGGSGAGLNFDAAGQPAGGFDGTSVVQTHMTNSRLTDPEVLEFRFPVRLES
                     YEIRKGSGGAGRWHGGDGGVRRVRFLEAMTASILSNGRHHGAFGLAGGATGRPGINRV
                     ERADGSVVALDHIGSVEMAPGDVFVVETPGGGGCGAEAEAAARIHAA"
BASE COUNT          370 a          923 c          896 g          393 t
ORIGIN      
        1 gcgcggcggc ccgaagtcat gcggccgcca cgcgcgcggc gatcgtcgtc gcgcgcgcgg
       61 cctcgccgag cagcacgtcg agcgcgggca cgtcggtgtc ccattcgacc agcgtcggca
      121 cggcggcgcc gaagcggcgc agcgcgtgct cgtagaccga ccacaccgcc ggccgcaccc
      181 ggctgccgtg atcgtcgatc gcgaggccgt cggcgtcggc atagcccgcg aggtgcacct
      241 cgccgacgat gcccaacggc agcgcgtcga cgaagccgca gcacgccgcg agcggatcgg
      301 ccacgccagc gttcagcgcg ttgaccatca ggttgttgac atcgagcagc atgccgcagc
      361 cgctgcgccg gcacagctgc gtgaagaact cgggctcgct catcgagtcg tcggcgaacg
      421 agacgtaggc cgacaggttc tcgaccagca gcgtgcgacc gagccgctcc tgcacctgcg
      481 acacgttggc caccatcacg gccagcgccg cctcggtgaa cgcgatcggc agcaggtcgt
      541 tcgcgtgcac gatcgggccg tcgggcacgc gcggcgcgcg cgcgaagctg gcgtggtccg
      601 agacgcgcac cggctcgatc gcgcgcgcca gaagcgccag ccgctccagg tgccaggggt
      661 ccaggccgac cgccgagccg agtgcgaggc cgacgccatg caggctgacc gcgtagtgct
      721 cgcgcgcccg gcgcagcacc gccagcgcgg cgccgccatc ggcgaagaag ttctcggaat
      781 ggacctcgac gaagccgagc ggcggcacgc tgtcgagcag cgcggtgtag tgcggttgac
      841 gcaggccgac gccggccggc accaggcctt cgcgcatcac ttcttcgcga tcagcgcctt
      901 ggcttcgtct tccgagatgc cgccgatctt cgcgcagctg cccttggcga cgtagttcca
      961 ttcatccggc gccatcgcgt tcttgttctg gccggcgcac gaatggctgc ccgacaggtt
     1021 ggcgcaatcg ttggtgcctt ccttcgcgat gccgaagcac ttttcctttt ccttgccttc
     1081 ggcggcgtgg gccgccgaca gcgcccccat cgcgaggacg gaagcgaggg cgctggaggc
     1141 gatcagtcgt gcgttcatgg tgtcggaatc tccggtggtt gagcgagagc gtgtcgacgc
     1201 gccggccggt gccgccattg tcgatcgacg acctgcctcg gtcgaaaagc ccctacgcga
     1261 ccttacgccg cgtggatgcg cgccgcggcc tcggcctcgg cgccgcagcc gccgccgccg
     1321 ggcgtttcga cgacgaacac gtcacccggc gccatctcga ccgagccgat gtggtcgagc
     1381 gcgacgacgc tgccatcggc acgctcgacg cggttgatgc cgggtcgtcc cgtcgcgccg
     1441 ccggccaggc cgaaggcgcc gtggtggcgg ccgttcgaca ggatcgacgc cgtcatcgcc
     1501 tcgaggaagc gcacccggcg cacaccaccg tcgccgccgt gccagcggcc ggcaccgccc
     1561 gaccctttgc ggatctcgta gctctccagc cgcaccggaa agcggaactc cagcacctcg
     1621 gggtcggtca gccgcgaatt cgtcatgtgg gtctgcacga ccgaggtgcc gtcgaagcca
     1681 cccgccggct ggcccgccgc gtcgaagttc agccccgcgc cgctgccgcc ggagatcgtc
     1741 tcgtagtact ggtggcgtgc gttaccgaag gtgaaattgt tcatcgtgca ctggcccgcc
     1801 gccatcacgc cgagcgcgcc gtagagggca ttggtgatgc aggtcgaggt ctcgacgttg
     1861 cccgccacca ccgaggccgg cggatgcggg ttgagcatcg agccgtcggg gatgatcacg
     1921 cgcaagggct tcaggcaacc ggcgttcagc gggatgtcgt cgttcaccag cgtgcggaac
     1981 acgtacagca ccgcggccat gcagaccgcg gtcggcgcgt tgaagttgtt cgccagctgc
     2041 gccgagctgc cggtgaagtc gatctcggcg ctgcgcgcgg ccgcgtcgac gcggatggcc
     2101 acgctgatct gcgcgccgtt gtccagcggc agcgtgaacg cgccgtcctt gagtttggtg
     2161 atgacgcggc gcaccgactc ttcggcgttg tcctgcacat ggcgcatgta ggcctgcacc
     2221 acgtccagcc cgaactgccg gaccatcctg cgcagttcct gcacgccctt ctcgttggcg
     2281 gcgatctgcg ccttcaggtc ggccagattc tgctgggggt tgcgcgacgg gaattcgccg
     2341 ctgcccagca gcgccagcat ctcggcctcg cgcagcgtgc cgcggtcgac cagcttgacg
     2401 ttgtcgatct gcacgccctc ttcgtcgatg cgggtcgaga acggcggcat cgagccgggc
     2461 gtgacgccgc cgatgtcggc gtggtggccc cgggagccga cgtagaacaa gattcgacct
     2521 tcgtcgtcgc cggcgggccc ggcgcccggc cgctccgaag ccgggcccgg actcccacgg
     2581 gg
//
LOCUS       DATMJB010000639         4578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_402267, whole genome shotgun sequence.
ACCESSION   DATMJB010000639 DATMJB010000000
VERSION     DATMJB010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402267"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..275)
                     /locus_tag="VNU48_13560"
     CDS             complement(63..275)
                     /locus_tag="VNU48_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12354.1"
                     /translation="MPGVFCDELQEAYFGAAQGDKVIRDFIGLARRAPDAIESCSAFS
                     VVASELSRITGQIDELVREALRASAA"
     gene            complement(556..1425)
                     /gene="nadC"
                     /locus_tag="VNU48_13565"
     CDS             complement(556..1425)
                     /gene="nadC"
                     /locus_tag="VNU48_13565"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWI12355.1"
                     /translation="MFDHDETLEQARARNIRDALMEDLGRGDWTGRLIPAGRRVQARL
                     MVRESAVLAGRDWFDGCVHALDPAARIAWHCAEGADMAADSLVCEIEADARALLSAER
                     PALNFLQLLSATATITRAHVRAIEGASPNPRGCAVLDTRKTLPGLRQAQKYAVRVGGG
                     QNQRLALWHGILIKENHIAAAGGVTAALAGAFALRSGVAIQIEVEGLDQLREALAAGA
                     TSVLLDNFTEPAMREAVAITAGRALLEVSGGVTLAQLRAIAATGVDRVSIGKLTKDVQ
                     AVDYSMRVVGQVA"
     gene            complement(1437..2495)
                     /gene="nadA"
                     /locus_tag="VNU48_13570"
     CDS             complement(1437..2495)
                     /gene="nadA"
                     /locus_tag="VNU48_13570"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HWI12356.1"
                     /translation="MSFTTLQVAVRDARRTDSARPDLRRQIAQELRARDAVLVAHYYV
                     DGDLQDLALETGGCVADSLEMARFGRAHSAKTLIVAGVRFMGETAKILSPTKRVLMPD
                     LEATCSLDLGCPPDEFAAFCDAHPDRTVVVYANTSAAVKARADWMVTSSCAVAIVRHL
                     QGLGQKILWAPDRHLGRYIQQETGADMLLWDGACIVHDEFKGLELELMRREHPQAQVL
                     VHPESPASVVAQADVVGSTSQLLKAVVDGTAPTYIVATDNGILHRMRQLAPGKTLLEA
                     PTAGQSATCKSCAHCPWMAMNELQGVLDCLLHGRGEIGVPEPVRGQALGCIERMLDFV
                     ATQPVPAAPGLVPNIGAA"
     gene            complement(2627..4576)
                     /locus_tag="VNU48_13575"
     CDS             complement(2627..4576)
                     /locus_tag="VNU48_13575"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HWI12357.1"
                     /translation="MQNGVAEVYGEGASYAVTPRQGYRFYGTGLSDYDALPVRRDDDL
                     DRWARDRDRRADTSPSARYVSPEVVGYEDLDANGSWRADPTYGSVWTPTRVAAGWTPY
                     HDGHWAWVDPWGWTWVDDAPWGFAVSHYGRWANFNGTWGWVPGPAREPAVYAPALVAF
                     VGGKNFQVSVSVGSAVAAAVGWFPLAPREVYRPSYPVSRGYFDSVNRSNAVIAPATIT
                     NVYNTTIVNNTTNIVKTVYVNQQVSGAVVAVPAQAFAQSQSVARAAVPLTREAALSAP
                     VAHVATVAPVQQSVHGGAPVALVKPPAREVAVVAHTAPPPPPVPFAEQQKHLAANPGK
                     PIDEAQRVQLKPAIVPGPQAKVSVIAAARASAPAAPPPATAPAPRTPEARKADAARGA
                     EAAVGGRPSDGSRSTAAPADASKAAAANADAAKAEAARAEAMKAEAAKAGAAKADAAK
                     ADAAKLEAARAEATRAADAAKADATKAEAGRVEAAKADAARADATRAAATRADLARAE
                     AAKADAARTDAAKAEAAKAELARSAAAKADASRAEAARVEAARAEAARGDAARAEANR
                     AAAARAEAGRAESAKAEAAKAAAARGEAARGEAARADAAARAAAPPRPTAAAPAPPRA
                     SSPRGGPDAKGEEEKKRPDEEPHKR"
BASE COUNT          574 a         1649 c         1625 g          730 t
ORIGIN      
        1 catggcggta cggcgctgca tcggagatcc ttgacgagga tggaagggga tggtggcggc
       61 gctcacgcgg ccgaagcgcg cagcgcttcg cgcacgagtt catcgatctg gccggtgatc
      121 cgcgacaact cgctcgccac gaccgagaag gcagagcaag actcgatggc atcgggcgca
      181 cgccgcgcga gcccgatgaa atcgcgaatg accttgtcgc cttgcgctgc gccgaagtaa
      241 gcctcctgca actcatcgca gaagacgccg ggcactcgcg ctgtgacgtg aagcgcctgc
      301 ggcccgaaag atcgatgagt gtgccgaaaa cctcgccaga gacgctcgct gtcttcagat
      361 tcttggtatc gggcgcttga gttctctcca ggtccagcat ccgagggctc catgtcgacg
      421 gtcgcgggcc gggaaggcga cccgcggtgt tgacccacgc tgaagcacgg tccgtaccag
      481 cgaaacggga ggctgtcgca ccggccgatg ggccggttca acgagccgag tcgttcttcc
      541 gcatgacgcg cccgctcacg cgacttgccc gaccacccgc atcgagtagt cgaccgcctg
      601 cacgtccttc gtcagcttgc cgatcgacac gcggtcgacg ccggtcgcgg cgatggcgcg
      661 cagctgcgcg agcgtgacac cgcccgagac ttcgagcagg gcccggcccg ccgtgatggc
      721 cacggcctcg cgcatcgccg gttcggtgaa gttgtcgagc agcacgctgg tcgcgcccgc
      781 cgcgagagcc tcgcgcaatt gatcgaggcc ctcgacctcg atctggatcg ccacgcccga
      841 tcgcagcgcg aacgcgccgg ccagcgccgc cgtcacgccg ccggccgcgg cgatgtggtt
      901 ctccttgatc agaatgccgt gccacagcgc gagccgctgg ttctgcccgc cgccgacgcg
      961 caccgcgtac ttctgtgcct ggcgcaggcc cggcagggtc ttgcgcgtgt cgagcacggc
     1021 gcagccgcgc gggttcggtg acgcgccttc gatcgcgcgc acatgggcgc gcgtgatcgt
     1081 cgcggtggcc gagagcaact gcaggaagtt cagcgccggc cgctcggccg agagcagggc
     1141 gcgggcgtcg gcctcgatct cgcagaccag gctgtcggcc gccatgtcgg cgccctcggc
     1201 gcagtgccag gcgatgcgcg ccgcggggtc gagcgcgtgc acgcagccat cgaaccagtc
     1261 gcggccggcc agcacggcgc tttcgcgcac catgagccgt gcctgcacgc ggcggcccgc
     1321 gggaatgagc cggccggtcc agtcgccacg gccgaggtct tccatcagcg cgtcgcgaat
     1381 gttgcgcgcg cgcgcctgct cgagggtctc gtcgtggtcg aacatggcgt gagcgctcag
     1441 gccgcgccga tgttcggcac gaggcccggc gccgccggca ccggctgcgt ggcgacgaag
     1501 tcgagcatgc gctcgatgca gccgagcgcc tggccgcgca cgggttcggg cacgccgatc
     1561 tcgccgcggc cgtgcagcag gcagtcgagc acgccttgca gttcgttcat cgccatccat
     1621 gggcagtgcg cgcagctctt gcacgtggca ctctggcccg cggtgggcgc ctcgagcagc
     1681 gtcttgcccg gcgcgagctg gcgcatgcgg tgcaggatgc cgttgtcggt ggcgacgatg
     1741 taggtcggtg ccgtgccgtc gaccacggcc ttcagcagct gcgaggtcga gcccaccaca
     1801 tcggcctgcg cgacgacgct cgccggcgac tccgggtgca ccagcacctg ggcctgtgga
     1861 tgctcgcggc gcatcagctc cagctcgagg cccttgaact cgtcgtgcac gatgcaggcg
     1921 ccgtcccaga gcagcatgtc ggcgccggtc tcttgctgga tgtagcggcc caggtgtcgg
     1981 tcgggtgccc acaggatctt ctgcccgagg ccctgcaggt ggcgcacgat ggcgaccgcg
     2041 cacgagctcg tcaccatcca gtcggcgcgc gccttcacgg cggcgctggt gttggcgtag
     2101 acgacgaccg tgcggtcggg atgggcgtcg cagaacgcgg cgaattcatc cggcgggcag
     2161 cccaggtcga gcgagcaggt cgcttcgaga tcgggcatca gcacacgctt ggtcggcgac
     2221 aggatcttgg cggtctcgcc catgaagcgc acgccggcga cgatcagcgt cttcgcgctg
     2281 tgggcgcggc cgaaacgggc catctcgagc gagtcggcga cgcagccgcc ggtctcgagc
     2341 gccaggtcct gcaggtcgcc gtcgacgtag tagtgggcga ccagcaccgc gtcgcgggca
     2401 cggagttctt gcgcgatctg ccgccgcaga tcgggccggg cggagtcggt gcgccgcgcg
     2461 tcgcggaccg ccacctggag tgtcgtgaag ctcatcgttc gggttgccaa tcggggaagc
     2521 cggtggcgga tcactctgac cgctctgatc ctatcgcagg tttcccttcg gtccgacggg
     2581 tgaagcggtg tcgcctgccg tgagatgcgg cgaggcgatg cgggccctag cgcttgtgcg
     2641 gctcttcgtc gggccttttt ttctcctcct cgcccttcgc gtcgggcccg cccctcggcg
     2701 aggacgcgcg cgggggcgca ggcgcggccg ctgtcggcct gggtggcgcg gcggcccttg
     2761 ccgccgcatc ggccctggct gcctcgcctc tcgctgcctc gcctctcgcc gcggcggcct
     2821 tggccgcttc ggccttcgcc gactcggctc gccccgcctc ggccctggcg gcggccgccc
     2881 tgttggcttc ggccctggcc gcgtcgcctc gggcggcctc ggcgcgggcc gcttccaccc
     2941 gcgcggcctc ggccctcgaa gcatccgcct tcgcggccgc cgaccgcgcc aactcggctt
     3001 tcgccgcttc tgccttcgcg gcgtcggttc gggccgcgtc tgccttggcg gcttccgctc
     3061 tggcgagatc cgccctcgtt gcggccgccc gggtcgcatc ggcccttgcc gcatcggcct
     3121 tggccgcttc gactcttccg gcttcggcct tcgtggcatc ggccttcgcc gcatccgctg
     3181 ctcgagtcgc ttcggccctg gccgcctcca gcttggcggc gtctgccttg gccgcatctg
     3241 ccttggctgc accggccttg gccgcttcgg ccttcatcgc ctccgccctg gccgcttctg
     3301 ccttcgcagc atcggcgttg gccgcagcgg ccttcgacgc atcggccggt gcggcggtcg
     3361 acctggagcc atcgctcggg cggccgccga cagcggcctc ggcaccacgc gccgcatcgg
     3421 ccttgcgggc ctcgggtgtc ctcggtgccg gggccgtcgc tggcggcggc gccgcgggtg
     3481 ccgacgcgcg ggcggcggcg atcacgctga ccttggcttg cgggccgggc acgatcgcgg
     3541 gcttcaattg cacgcgctgc gcttcgtcga tgggtttgcc cggattcgcc gcaagatgct
     3601 tttgctgctc ggcgaagggc accggcggcg gcggcggcgc cgtgtgcgcg acgaccgcca
     3661 cctcgcgggc cggcggcttg accagtgcca ccggcgcgcc gccgtgcacg ctctgctgca
     3721 ccggcgccac cgtggcgacg tgcgcgaccg gcgcgctgag cgcggcttct ctggtcagcg
     3781 gcacggcggc tctggccacc gactgcgact gcgcgaaggc ctgcgccggc acggcgacca
     3841 cggcaccgct cacctgctgg ttgacataga ccgtcttcac gatgttcgtc gtgttgttga
     3901 cgatggtcgt gttgtagacg ttggtgatgg tcgccggcgc gatcaccgca ttgctgcgat
     3961 tgacgctgtc gaaatagccg cgactcaccg gatacgacgg tcgatagacc tcgcgcggcg
     4021 cgagcgggaa ccagccgacc gcggcggcca cggcgctgcc cacggagacc gagacctgga
     4081 agttcttgcc gccgacgaag gccaccagcg ccggcgcata caccgccggt tcgcgcgccg
     4141 ggccgggcac ccagccccag gtgccgttga agttggccca gcggccgtag tgcgacacgg
     4201 caaatcccca gggggcgtcg tcgacccagg tccagcccca cggatcgacc caggcccagt
     4261 ggccgtcgtg gtacggcgtc cagccggcgg cgacacgggt cggcgtccac acgctgccgt
     4321 aggtcggatc ggcgcgccag ctgccgttgg cgtcgaggtc ctcgtagccg acgacttccg
     4381 gtgacacgta gcgagccgac ggcgacgtgt cggcgcgacg atcgcgatcg cgcgcccagc
     4441 ggtcgaggtc gtcgtcgcgt cgcacgggca gcgcgtcgta gtcggacaag ccggtgccgt
     4501 agaagcgata gccctgccgc ggcgtgaccg catacgaagc cccctcgccg tagacctcgg
     4561 ccacgccgtt ctgcaccg
//
LOCUS       DATMJB010000640         4369 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_265968, whole genome shotgun sequence.
ACCESSION   DATMJB010000640 DATMJB010000000
VERSION     DATMJB010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265968"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VNU48_13580"
     CDS             <1..1068
                     /locus_tag="VNU48_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI12358.1"
                     /translation="FWCTADVGWVTGHTYITYGPLAVGATEIMFEGIPTHPDAGRFWK
                     MIQDHKVSIFYTAPTAIRSLIKAAEGTAAVHPDRYDLSSLRILGTVGEPINPAAWEWY
                     HKHIGGGRCPIVDTFWQTETGGHMITPLPGATPLVPGSCTLPFPGIAAAIVDEAGHDV
                     PNGTAGILVVKKPWPSMIRTIWGDPERFKKSYYPDDFKGKYYLAGDGAIRDAKTGYFT
                     ITGRIDDVLNVSGHRMGTMEIESALVSCTELVAEAAVVGRPDDTTGEAIVAFVVLKRT
                     RPSGDEAKAIAKQLRDWVGKEIGPIAKPKDIRFGDNLPKTRSGKIMRRLLRSVAKGEA
                     ITQDTSTLENPAILEQLGANY"
     gene            1148..2539
                     /locus_tag="VNU48_13585"
     CDS             1148..2539
                     /locus_tag="VNU48_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HWI12359.1"
                     /translation="MQLSASAFKAYDIRGVVGRDIDAAFAEQLGRAFGSAAIAAGERA
                     VAVGRDGRVSGPMLVQALMRGLASTGLDVVDLGPVTTPMLYYVAATRGAHGCRSGVMV
                     TGSHNPKDYNGFKMVLAGEAIHGEAIQALRRRMLAADHVKGTGRIGTMDILAEYRARI
                     TDDCRLARPMKIVVDSGNGIPGASAPGILRALGCEVVELYSEVDGDFPNHHPDPSKPE
                     NLAELMRTVRETGAELGLAFDGDGDRLGVVTNDGRIIYPDRQLMLFASDILARVKGGT
                     IIFDVKCTQRLAPLIRRAGGTPLMWKTGHSLMKAKLRETGAPIAGEMSGHIFFAERWY
                     GFDDATYTAARLLEILSREKDPSGVLNALPTSFSTPELNVACAEGEPKRVVDDLLRAA
                     DFPGASEIITIDGLRVDYDDGFGLIRASNTTPVLVLRFEGHTAAALQRIEIAMMAALR
                     AVKPDAQVATAAH"
     gene            2543..3529
                     /gene="waaC"
                     /locus_tag="VNU48_13590"
     CDS             2543..3529
                     /gene="waaC"
                     /locus_tag="VNU48_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HWI12360.1"
                     /translation="MRVLIVKLSSLGDVVHAMPVVHDIRSALPEATIAWVVEPGFAPL
                     VRRVEGVAEVIECALRRWSGAWWTAATRAEWRALRSRLQRERFDAVLDLQGLTKSALI
                     ARLARLTPGGARYGLANRTDGASFEAPARWLVDRAIRVEPRTHALDRSRVLAARALGY
                     AFAGPPRFGLRAAAAAARADAPTLVLVHGSSRDDKLWPEPAWIEFGRRAIAEGWRIAL
                     PHAGASELARAQRIAGALGAQAQVWPAMKLDALVDRLGATQGVIGVDSGLSHIAVALD
                     LPHVQLYNWPTAWRTGPQAAHGHRHQVSVQAQPTPSVDAVWAAWIQVREAAA"
     gene            3588..>4369
                     /locus_tag="VNU48_13595"
     CDS             3588..>4369
                     /locus_tag="VNU48_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HWI12361.1"
                     /translation="MNRVREALARGAYALLLRLLAPVYALRLWWRGGAEPLYRERIGE
                     RFGFYGDVRSAGWVWVHAVSLGETLAAAALIDALRIERPVMNLLLTHGTATGREAGRA
                     LLRDGDRQTWLPYDTPGAARRFFAQFRPAVGVLMETEVWPHLLLAAQDARVPMVLANA
                     RLSAKSFARGRRLHAVLRPAIDSVALVLAQTDDDAQRLRASGARAVEVCGNLKYDLTP
                     DAALLARGAAWRAQAPRAVVLAAITREGEEALLLEAWTRQPVA"
BASE COUNT          629 a         1549 c         1605 g          586 t
ORIGIN      
        1 ttctggtgta ccgccgacgt gggctgggtc accgggcaca cgtacatcac gtacgggccg
       61 ctcgcggtgg gcgcgaccga gatcatgttc gagggcatac ccacccatcc cgacgccggc
      121 cggttctgga agatgatcca ggaccacaag gtcagcatct tctacaccgc gccgacggcg
      181 atccgctcgc tgatcaaggc cgccgagggc accgcggcgg tccaccccga ccgttacgat
      241 ctgagttcgc tgcgcatcct cggcacggtg ggcgagccga tcaacccggc ggcgtgggag
      301 tggtaccaca agcacatcgg tggcggccgc tgcccgatcg tcgacacctt ctggcagacc
      361 gaaaccggcg gccacatgat cacgccgctg ccaggcgcga cgccgctggt gccgggctcg
      421 tgcaccttgc cgttccccgg cattgcggcg gcgatcgtcg acgaagccgg gcacgacgtg
      481 ccgaacggca cggccggcat cctggtcgtc aagaagccgt ggccgagcat gatccgcacg
      541 atctggggcg acccggaacg cttcaagaaa agctactacc cggacgactt caagggcaag
      601 tactacctcg ccggcgacgg tgcgatccgc gacgcgaaga ccggctactt cacgatcacc
      661 ggccgcatcg acgacgtgct gaacgtcagc ggccaccgca tgggcacgat ggagatcgag
      721 tcggcgctgg tgtcgtgcac cgagctggtg gccgaggcgg cggtcgtcgg gcggcccgac
      781 gacacgaccg gcgaggcgat cgtcgcgttc gtggtgctca agcgcacgcg cccgagcggc
      841 gacgaggcga aagccatcgc gaagcagttg cgcgactggg tcggcaagga gatcggcccg
      901 atcgcgaagc cgaaggacat ccgcttcggc gacaacctgc cgaagacacg cagcggcaag
      961 atcatgcggc gactgttgcg ctcggtcgcg aagggcgagg cgatcacgca ggacacctcg
     1021 acgctggaga acccggcgat cctcgagcag ctcggcgcga actactgagc gagcgcgcgg
     1081 ggcgcgcggg ccgggtggcg ccggtcccta caatcggcgc acttcacgcg accgactctc
     1141 cttgcccatg cagctcagcg cctccgcctt caaggcctac gacatccgcg gcgtcgtcgg
     1201 acgggacatc gatgcggcct tcgccgagca gctcggccgc gccttcggca gcgccgcgat
     1261 cgcggccggc gagcgcgcgg tcgcggtcgg gcgcgacggg cgcgtctcgg ggccgatgct
     1321 ggtgcaggcg ctgatgcgcg ggctggcatc gaccgggctg gatgtcgtcg acctcgggcc
     1381 ggtgaccacg ccgatgctgt actacgtcgc ggcgacgcgc ggcgcgcatg gctgccgcag
     1441 cggcgtgatg gtgaccggca gccacaaccc gaaggactac aacggcttca agatggtgct
     1501 ggcgggcgag gcgatccacg gcgaggcgat ccaggcgctg cgccggcgca tgctggccgc
     1561 ggaccatgtg aagggcacgg gccgcatcgg cacgatggac atcctggccg agtaccgcgc
     1621 ccgcatcacc gacgactgcc ggctggcgcg cccgatgaag atcgtcgtcg actcgggcaa
     1681 cggcattccc ggcgcgtcgg cgccgggcat cctgcgcgcg ctcggctgcg aggtggtcga
     1741 gctctactcc gaggtcgacg gcgacttccc gaaccatcac cccgacccga gcaagcccga
     1801 gaacctggcc gagctgatgc gcacggtgcg cgagaccggt gccgaactcg gcctggcgtt
     1861 cgacggcgac ggcgaccgcc tgggcgtcgt cacgaacgac ggccggatca tctacccgga
     1921 ccgccagctg atgctgttcg cgagcgacat cctggcgcgc gtgaagggcg gcacgatcat
     1981 cttcgacgtc aagtgcacgc agcgcctggc gccgctgatc cgtcgcgccg gcggcacgcc
     2041 gctgatgtgg aagaccggcc actcgctgat gaaggccaag ctgcgcgaga ccggcgcgcc
     2101 gatcgccggc gagatgagcg gccacatctt cttcgccgag cgctggtacg gcttcgacga
     2161 cgcgacctac accgcggcgc ggctgctcga gatcctgtcg cgtgagaagg acccgagcgg
     2221 cgtgctgaat gcgctgccga cgagcttctc gacgcccgag ctgaacgtgg cctgcgccga
     2281 aggcgagccg aagcgcgtcg tcgacgacct gctgcgcgcc gcggacttcc ccggcgcgag
     2341 cgagatcatc acgatcgacg gcttgcgcgt cgactacgac gacggcttcg gcctgatccg
     2401 cgcgtcgaac acgacgccgg tgctggtgct gcgcttcgaa ggccacaccg cggcggcgct
     2461 gcagcgcatc gagatcgcga tgatggcggc cttgcgcgcg gtcaagccgg acgcgcaggt
     2521 ggcgaccgcg gcgcactgaa cggtgcgcgt gctcatcgtc aagctgtcgt cgctcggcga
     2581 tgtggtgcat gcaatgccgg tggtgcacga cattcgctcg gcgctgcccg aggcgacgat
     2641 agcctgggtc gtcgaacccg gcttcgcgcc gctggtgcgc cgcgtcgagg gcgtcgccga
     2701 agtcatcgaa tgcgcgctgc gacgctggag cggtgcgtgg tggaccgccg cgacgcgcgc
     2761 cgaatggcgc gccttgcgca gccggctgca gcgcgagcgc tttgatgcgg tgctcgacct
     2821 gcaggggctg accaagtcgg cgctgatcgc gcggctcgcg cggctgaccc ccggcggcgc
     2881 gcgctacggc ctggccaacc gcaccgacgg cgcgagcttc gaggcgccgg cgcgctggct
     2941 cgtcgatcgc gcgatccgag tcgagccacg cacccacgcg ctcgaccgct cgcgtgtgct
     3001 ggcggcgcgg gcactgggct atgcgttcgc cggcccgccg cgcttcgggc tgcgtgctgc
     3061 cgctgcggcg gcgcgcgccg atgcgccgac gctggtgctg gtccacggca gctcgcgcga
     3121 cgacaagctc tggcccgagc ccgcgtggat cgagttcggt cgccgtgcca tcgccgaggg
     3181 ctggcgcatc gcgctgccgc acgccggcgc gtccgaactc gcgcgcgcgc agcgcatcgc
     3241 cggcgcgctc ggcgcgcagg cgcaggtctg gcccgcgatg aagctcgacg cgctggtcga
     3301 ccggctcggc gcgacgcaag gtgtgatcgg cgtcgacagc ggcctgagcc acatcgcggt
     3361 cgccctggac ctgccgcacg tgcagctcta caactggccg accgcgtggc gcaccgggcc
     3421 gcaggccgcg cacgggcacc gccaccaggt gtcggtgcag gcgcagccga cgccgtcggt
     3481 cgacgcggtc tgggccgcgt ggattcaggt gcgtgaggcc gcggcttgag cccgcccggc
     3541 cgcccgaagg gcgaaatcac cgcagtgcga agcacggagg ttgcctgatg aaccgcgtgc
     3601 gcgaagcgct ggcgcgtggc gcctacgcgc tgctgctgcg cctgctggcg ccggtctacg
     3661 cgctgcgcct gtggtggcgc ggcggcgccg agccgctgta ccgcgaacgc atcggcgagc
     3721 gcttcgggtt ctacggcgac gtgcgcagcg ccggctgggt ctgggtccac gcggtgtcgc
     3781 tcggcgagac gctggcggcg gcggcgctga tcgatgcgct gcgcatcgag cgccccgtca
     3841 tgaacttgct gctgacccac ggcaccgcca ccggccgcga agccgggcgc gcgctgctgc
     3901 gcgacggcga ccgccagacc tggctgccct acgacacgcc gggcgcggcg cggcgcttct
     3961 tcgcgcagtt ccgccccgcc gtcggcgtgc tgatggagac cgaggtctgg ccgcacctgc
     4021 tgctcgccgc gcaggacgcg cgtgtgccga tggtgctcgc gaacgcgcgc ctgagcgcga
     4081 agagcttcgc gcgcggccgg cgcctgcacg cagtgctgcg gccggcgatc gacagcgtcg
     4141 cgctggtgct cgcgcagacc gacgacgacg cgcagcgcct gcgcgcctcg ggcgcgcgcg
     4201 cggtcgaggt ctgcggcaac ctgaagtacg acctgacgcc cgacgccgcg ctgctcgcgc
     4261 gcggcgcagc gtggcgcgcg caggcgccgc gcgccgtggt gctggccgcg atcacccgcg
     4321 agggcgagga ggcgctgctg ctcgaggcct ggacgcgcca gccggtcgc
//
LOCUS       DATMJB010000641         2315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_81774, whole genome shotgun sequence.
ACCESSION   DATMJB010000641 DATMJB010000000
VERSION     DATMJB010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81774"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /gene="guaA"
                     /locus_tag="VNU48_13600"
     CDS             complement(<1..1399)
                     /gene="guaA"
                     /locus_tag="VNU48_13600"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWI12362.1"
                     /translation="MHDKILILDFGSQVTQLIARRVREAHVFCEIHPNDVSDAFIREF
                     APKGIILSGSHASTYEEHELRAPPAVWELGVPVLGICYGMFTMAVQQGGQVEAADHRE
                     FGYAEVRAHGHTKLLSGIEDFSTAEGHGMLKVWMSHGDKVTALPPGFKLMASTKSCPI
                     AGMADESRGYYGVQFHPEVTHTQKGRELLNRFVLDIAKARPDWVMGNYVDEAVARIRE
                     QVGDEEVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGALVMEMFAGRL
                     HAKVIAVDASAQFLGALAGVTDPEAKRKIIGREFVEVFQAEATKLKDAKWLAQGTIYP
                     DVIESGGAKTKKATSIKSHHNVGGLPETLGLKLLEPLRELFKDEVRELGVALGLPHDM
                     VYRHPFPGPGLGVRILGEVKPPFADLLRRADAIFIEELRSTVDAATGKSWYALTSQAF
                     AVFLPVKSVGVMGDGR"
     gene            complement(1419..>2315)
                     /locus_tag="VNU48_13605"
     CDS             complement(1419..>2315)
                     /locus_tag="VNU48_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWI12363.1"
                     /translation="VANAYVADITAPADRAKAFGKLGAMLGMGFILGPVLGGLLGGIS
                     LHLPFFAAGTLACVNWLYGYFVLPESLPPERRRPFVLREANPINALRGLTALKGVGSL
                     VVVIGLSGLAQFMTHTSWVLYTTFKFGWGPTQNGWSLFAIGLMSVLVQGVLLAKLLKR
                     FSPQRLAIMGLISSSICFVMWGLATEGWMLYAVIFANVLGFAVSASIQSLISNAADPG
                     AQGRTAGAVGSLNSMMAVIAPAIGAPLLATVSHLPHGDWRIGAPFYFCALLQAASTVI
                     AVRHFRGERRQRELLAAAASTT"
BASE COUNT          372 a          821 c          770 g          352 t
ORIGIN      
        1 tacggccgtc gcccatcacg ccgacgctct tgaccggcag gaacaccgcg aaggcctggc
       61 tcgtcaacgc gtaccagctc ttgccggtgg cggcgtcgac cgtgctgcgc aactcctcga
      121 tgaagatcgc atcggcgcgg cgcagcaggt cggcgaaagg gggcttgacc tcgcccagga
      181 tgcgcacgcc caggcccggc ccggggaacg ggtgccggta caccatgtcg tgcggcagcc
      241 cgagcgcgac gccgagctcg cgcacctcgt ccttgaacag ttcgcgcagc ggttcgagca
      301 gcttcaggcc cagcgtctcg ggcagcccgc cgacgttgtg gtggctcttg atcgaggtcg
      361 ccttcttcgt cttcgcgccg ccgctctcga tcacgtccgg gtagatcgtg ccctgcgcca
      421 gccacttcgc gtctttcagc ttcgtcgcct cggcctgaaa cacctcgacg aactcgcggc
      481 cgatgatctt gcgcttggcc tcggggtcgg tcacgccggc cagtgccccg aggaactgcg
      541 cactcgcatc gaccgcgatc accttcgcgt gcagccgccc ggcgaacatc tccatcacga
      601 gcgcgccttc gttcaggcgc agcaggccgt ggtcgacgaa cacgcaggtc agctggtcgc
      661 cgatcgcgcg gtggatcagc gccgcggcca cgctcgaatc gacgccgccc gacagcccga
      721 ggatcacctc ttcgtcgccg acctgttcgc ggatgcgcgc gaccgcctcg tcgacgtagt
      781 tgcccatcac ccagtcgggc ctggccttcg cgatgtcgag cacgaagcgg ttcagcagct
      841 cgcggccctt ctgcgtgtgc gtgacctcgg ggtggaactg cacgccgtag tagccgcgcg
      901 attcgtcggc catgccggcg atcgggcagc tctttgtgga ggccatcagc ttgaagccgg
      961 gcggcagcgc ggtgaccttg tcgccgtggc tcatccagac cttcagcatg ccgtggcctt
     1021 cggcggtgct gaaatcctcg atcccgctca acagcttcgt gtggccgtgt gcgcgcacct
     1081 cggcgtagcc gaactcgcga tggtcggcgg cctcgacctg cccgccctgc tgcaccgcca
     1141 tcgtgaacat gccgtagcag atgcccagca ccggcacgcc gagttcccac acggccggcg
     1201 gcgcgcgcag ctcgtgctct tcgtaggtcg acgcatgact gcccgacagg atgatgccct
     1261 tgggcgcgaa ttcgcgaatg aacgcgtcgc tcacgtcgtt cgggtggatc tcgcagaaca
     1321 cgtgcgcctc gcgcacgcgg cgcgcgatca gctgcgtgac ctgggagccg aagtcgagga
     1381 tcaggatctt gtcgtgcatg gtggtggtga aggcaagctc aggtcgtcga agcggcggcc
     1441 gcgagcagct cgcgctggcg gcgttcgccg cggaagtggc gcaccgcgat cacggtcgag
     1501 gccgcctgca gcagcgcgca gaagtagaac ggcgcgccga tgcgccagtc gccgtgcggc
     1561 aggtgcgaca cggtggccag cagcggcgcg ccgatggccg gtgcgatcac cgccatcatg
     1621 ctgttcagcg agcccaccgc gccggccgtg cggccctgcg cgcccgggtc cgcggcgttc
     1681 gagatcaggc tctggatcga cgccgacacg gcgaagccga gcacgttcgc gaagatcacc
     1741 gcgtagagca tccagccctc ggtcgccagg ccccacatca cgaagcagat gctcgacgag
     1801 atcagcccca tgatcgcaag gcgctgcggc gagaagcgct tcagcagctt tgcgagcagc
     1861 accccctgca ccagcaccga catcaacccg atcgcgaaca gcgaccagcc gttctgcgtc
     1921 ggcccccagc cgaacttgaa ggtcgtgtac agcacccacg aggtgtgcgt catgaactgc
     1981 gccaggccgc tcaagccgat cacgacgacg agcgagccga cgcccttcag cgcggtcagc
     2041 ccgcgcagcg cattgatcgg gtttgcctcg cgcagcacga agggccggcg gcgctcgggc
     2101 ggcagcgact ccggcagcac gaagtagccg tacagccagt tcacgcaggc cagcgtgccg
     2161 gccgcgaaga acggcaggtg caggctgatg ccgccgagca gcccgcccag caccgggccg
     2221 aggatgaagc ccatgccgag catcgcgccg agcttgccga aggccttggc gcgatcggcc
     2281 ggcgcggtga tgtcggcgac atacgcgttc gcgac
//
LOCUS       DATMJB010000642         3502 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_562311, whole genome shotgun sequence.
ACCESSION   DATMJB010000642 DATMJB010000000
VERSION     DATMJB010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562311"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..92
                     /locus_tag="VNU48_13610"
     CDS             <1..92
                     /locus_tag="VNU48_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWI12364.1"
                     /translation="KKLGFENARALAGGLEAWRAANLPVEKTA"
     gene            114..380
                     /gene="grxC"
                     /locus_tag="VNU48_13615"
     CDS             114..380
                     /gene="grxC"
                     /locus_tag="VNU48_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HWI12365.1"
                     /translation="MQTVKMYSTQVCPYCVHAKALLKKRGVEAIEDIRVDLDPAQRRT
                     MMELTGRRTVPQIFIGETHVGGCDELVALDRRGALMPLLAGSGA"
     gene            471..953
                     /gene="secB"
                     /locus_tag="VNU48_13620"
     CDS             471..953
                     /gene="secB"
                     /locus_tag="VNU48_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HWI12366.1"
                     /translation="MADDNNPVFQIQRIYLKDLSLEQPNSPQILLEQGQPQVEINLGL
                     GAAPIGDSVYEVTVTATVTTKVNDKVLFLVEAKQGGIFEIRNLPDEQLQPIIGIACPG
                     IIYPYLRAIVSDVCTRAGFPPVLLSEVNFQAMFEAQQAQAAGQPNGSGSNGSGIITSA
                     "
     gene            1080..2087
                     /locus_tag="VNU48_13625"
     CDS             1080..2087
                     /locus_tag="VNU48_13625"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWI12367.1"
                     /translation="MNLTVLGAGAWGTALAASTAARHPTRLWARDAAQAASIHATRVN
                     AAYLPGARLPDELQVDADFEAALAHARTGLVIVATPMAGLDEMLRRIGTRAGGVLWLS
                     KGFEPGSGRLGHEIARAVDPQARVGVLSGPSFAIEVARGQPTALVAASADATLCEEAV
                     DALHSESLRIYTSDDPVGVEVGGAVKNVLAIATGIADAMPGLAGSPPGLGLNARAALI
                     TRGLAEMTRLGVALGARPETFMGLSGLGDLVLTATGDLSRNRSVGRLLAQGLPLARIL
                     SELGHVAEGVHSAAMVLQRAQALGVEMPIVQAVVAVLDGHLTPARALEQLMGRGARAE
                     T"
     gene            2110..3081
                     /locus_tag="VNU48_13630"
     CDS             2110..3081
                     /locus_tag="VNU48_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HWI12368.1"
                     /translation="MQRRRFLQTTLAGATTLAAPAFAQAWPNHPIKLVVPFPPGSSPD
                     FIGRLIAEPLARALGQAVVVDNRPGAGGNIGTGAVARAEPDGYTLLFTIQGPLVTAPL
                     LTKGLNYDPVRDLAPVTLVATSPNVLVVDPKLGANSLADFVRVAKQKKGQLNYGSVGL
                     GSASHLAMELFKTRAGIDLLHVPYQGFPQIVGAILAGQVQAAFMVPGLAMGQVRAGKL
                     QALGVTTPGRAGSLPELPSFVEQGYPDFEAISWQAVLAPASTPKAIVDRVAHELIRII
                     KSDEVRGKMLGQYFSAAGTAPEALAGLMKTERERWARVIKAAGVQPE"
     gene            complement(3137..>3502)
                     /locus_tag="VNU48_13635"
     CDS             complement(3137..>3502)
                     /locus_tag="VNU48_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HWI12369.1"
                     /translation="GFSMDDRLLRRAGLDYHEYAPVQRHASWAAFVAQMQPDPQRLFA
                     FTTRGSRPFADIAWRAGDWLVFGSETAGLPGALRDAFDEARRVRLPMRADQRSLNLSN
                     AVAVAVFEAWRQNGYAGGG"
BASE COUNT          526 a         1287 c         1228 g          461 t
ORIGIN      
        1 tgaagaagct cggcttcgag aacgcgcgcg cgctggccgg cgggctcgaa gcctggcgcg
       61 ccgcgaacct gccggtcgag aagacggcct gaacccggga cgccgccgca aaaatgcaaa
      121 ccgtcaagat gtattcgacc caggtctgcc cgtactgcgt ccatgcgaaa gccctgctga
      181 agaagcgcgg cgtcgaggcc atcgaggaca tccgtgtcga cctcgacccg gcccagcgcc
      241 gcacgatgat ggagctcacc ggccggcgca ccgtgcccca gatcttcatc ggcgagaccc
      301 atgtcggtgg ctgcgatgaa ctggtcgcgc tcgaccggcg cggcgcgctg atgccgctgc
      361 tcgccggcag cggcgcctga cgccggccgc ccctggcgcg cacggcgata atcgccgccg
      421 cacacacgct cgccgctgcg gccgagccca ccctccaacc cgagaccccc atggctgacg
      481 acaacaaccc ggtgttccag atccagcgca tctacctgaa ggatctgtcg ctcgagcaac
      541 ccaattcgcc gcagatcctg ctcgagcagg gccagccgca ggtcgagatc aacctcggcc
      601 tgggcgccgc gccgatcggc gacagcgtct acgaagtgac cgtcaccgcg accgtcacga
      661 ccaaggtcaa cgacaaggtg ctgttcctgg tcgaagccaa gcagggcggc atcttcgaga
      721 ttcgcaacct gccggacgag cagctgcagc cgatcatcgg catcgcctgc ccgggcatca
      781 tctacccgta cctgcgcgcg atcgtctccg acgtctgcac gcgcgccggc ttcccgccgg
      841 tgctgctgtc ggaagtgaac ttccaggcga tgttcgaggc gcagcaggca caggcagccg
      901 gccagccgaa cggcagcggc tcgaacggct ccggcatcat cacctcggcg tgatgacgac
      961 cccaaggcgc cttcggcgcc gtcccccgag gggagctcag gccgccatgg tccacgtagt
     1021 gggccacctc gtggcccttg gcggcccggc gctggcctga gatctgtcga gggcgcacga
     1081 tgaacctcac ggtcctcggt gccggcgcct ggggcaccgc gctcgcggcg agcaccgcgg
     1141 cgcggcatcc gacgcggctg tgggcgcgcg atgccgcgca ggccgcgagc attcacgcga
     1201 cacgggtgaa cgccgcgtat ctccctggcg cgcggctgcc ggacgaactg caggtcgacg
     1261 ccgacttcga ggccgcgctc gcgcatgcgc gcaccggcct cgtgatcgtg gccacgccga
     1321 tggccggtct ggacgagatg ctgcgccgca tcggcacgcg cgctggcggc gtgctgtggc
     1381 tcagcaaggg tttcgagccg ggcagcggcc ggctcggcca cgagatcgca cgcgcggtcg
     1441 acccacaggc acgagtcggg gtgctgtcgg gcccgagctt cgcgatcgag gtcgcgcgcg
     1501 gccagccgac cgcactggtc gccgccagcg ccgatgcgac gctgtgcgag gaggcggtcg
     1561 acgcgctgca cagcgagagc ctgcgcatct acacctcgga cgatccggtc ggcgtcgagg
     1621 tcggcggcgc ggtcaagaac gtgctggcga tcgcgaccgg cattgccgac gccatgccgg
     1681 ggctcgccgg tagcccgccc ggcctgggcc tgaacgcccg cgcggcgctg atcacacgcg
     1741 gcctggccga gatgacgcgt ctcggtgtcg cgctcggcgc gcgccccgaa accttcatgg
     1801 gcctgtcggg cctgggcgac ctggtgctga ccgccaccgg cgacctgtcg cgcaaccgca
     1861 gcgtcggccg attgctcgcg caggggctgc cgctggcgcg catcctgagc gaactcggcc
     1921 acgtcgccga gggcgtgcac agcgccgcga tggtgctgca gcgggcccag gcgctgggcg
     1981 tcgagatgcc gatcgtgcag gccgtcgtcg cggtgctcga cggccacctc acgccggcgc
     2041 gcgcgctcga acaactgatg ggccgcggcg cgcgcgccga gacctgaccg acggcccgaa
     2101 gccgcaccga tgcagcgccg ccgcttcctg cagaccacgc tggccggcgc cacgacgctc
     2161 gcggcgccgg ccttcgcgca agcctggccg aatcacccga tcaagctggt cgtgccgttc
     2221 ccgccgggca gctcgcccga cttcatcggc cgtctgatcg ccgagccgct ggcccgcgca
     2281 ttgggccagg ccgtggtcgt cgacaaccgg cccggtgccg gcggcaacat cggcaccggc
     2341 gcggtggcgc gcgccgagcc cgacggctac accttgctgt tcacgatcca ggggccgctg
     2401 gtgaccgcgc cgctgctgac caaggggctg aactacgacc cggtgcgcga cctcgcaccg
     2461 gtcacgctgg tcgcgacctc gccgaacgtg ctggtcgtcg acccgaagct cggcgcgaac
     2521 tcgctcgccg atttcgtgcg cgtcgcgaag cagaagaagg gccagctgaa ctacggcagc
     2581 gtcggcctcg gcagcgcctc gcacctggcg atggagctct tcaagacgcg cgccggcatc
     2641 gacctcttgc acgtgcccta ccagggcttc ccgcagatcg tcggcgcgat cctggccggc
     2701 caggtgcagg ccgcgttcat ggtgccgggc ctcgcgatgg ggcaggtgcg cgccggcaag
     2761 ctgcaggcgc tgggcgtcac gacgccgggc cgcgcgggct cgctgcccga gttgccgagc
     2821 ttcgtcgagc agggctatcc ggacttcgag gcgatctcgt ggcaggccgt gctggcgccg
     2881 gccagcacgc ccaaggcgat cgtcgatcgg gtcgcacacg agctgatccg catcatcaag
     2941 agcgacgagg tgcgcggcaa gatgctgggc cagtacttca gcgccgccgg caccgcaccc
     3001 gaggcgctgg ccgggctgat gaagaccgag cgcgagcgct gggcccgagt catcaaggct
     3061 gcgggcgtcc agcccgagtg atggagcacc cggtgacggc gtaccgtcac cacccgccgg
     3121 gcgctggccg cgcgcgtcag ccgccgcccg cgtagccgtt ctgccgccag gcttcgaaca
     3181 ccgcgaccgc caccgcgttg ctcagattca ggctgcgctg atcggcgcgc atcggcagac
     3241 gcacgcgacg ggcctcgtcg aaggcgtcgc gcagcgcgcc gggcagcccg gcggtttcgg
     3301 aaccgaacac gagccagtcg ccggcacgcc aggcgatgtc ggcgaaaggc cggctgccgc
     3361 gcgtcgtgaa cgcgaacagg cgctgcgggt ccggctgcat ctgcgcgacg aacgcggccc
     3421 acgacgcgtg gcgctgcacc ggcgcgtact cgtggtagtc gagtccggca cgccgcagca
     3481 gccggtcgtc catcgagaag cc
//
LOCUS       DATMJB010000643         2395 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_511924, whole genome shotgun sequence.
ACCESSION   DATMJB010000643 DATMJB010000000
VERSION     DATMJB010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511924"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VNU48_13640"
     CDS             <1..441
                     /locus_tag="VNU48_13640"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HWI12370.1"
                     /translation="CRPWGRIPMTVQMVLLPVFVQVGLTLALLLAMASARTRALTARE
                     TSLKDIALGQPNWPTRVTQFSNCYANQFEIPLLFYILIAIALPLRHADLVIVLLSWVF
                     VVTRFAHAGIFVTSNDVRTRSLAWFAGVLVVFAMWVWFALKILL"
     gene            473..1771
                     /locus_tag="VNU48_13645"
     CDS             473..1771
                     /locus_tag="VNU48_13645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HWI12371.1"
                     /translation="MTPAARLSAAIELIETIDAQRVPAAKALKEWGTAHRYAGSGDRA
                     AISGLIWDVLRRRSSSAWIMEEDTPRARLLGMLRLERQLDADAISALCDGGRFAPAPL
                     TDGERAALTLRSLVDAPAHIAGDYPEWLDGHLAQVFGDDRVAEATAMASRAPLDLRVN
                     TLKANREKMLPSLAHLGAQPTPWSPLGLRIELGADARNPGIHAEQDFIKGAIEVQDEG
                     SQLATLLSNAKPGEQVIDLCAGAGGKTLALAAMMQGKGRLIATDHDKRQLAPIYERLS
                     RAGAHNADVRTPKGPNDTLSDIHASADLVLIDAPCTGTGTWRRNPDAKWRMRPGALEV
                     RLKDQVTVLDRAAALVKPGGRIAYITCSVLPQENGEQVRGFVVRHPEFSITPPSQTVG
                     VLWDKAEDFASAALESAEGWLMTPRRTGTDGFFVSVLKRN"
     gene            1949..>2395
                     /locus_tag="VNU48_13650"
     CDS             1949..>2395
                     /locus_tag="VNU48_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011914102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HWI12372.1"
                     /translation="MASAHDKILIVDFGSQVTQLIARRVREEGVYSEIVPFQKAEAAF
                     AAMKPKAVILSGGPESVHEAGSPRAPQAIFDSGVPVLGICYGQMAMAAQLGGEVEGGH
                     HREFGRADVEVKAASNLFESTWSMGEKHQVWMSHGDRITKMPPGFTV"
BASE COUNT          399 a          768 c          809 g          419 t
ORIGIN      
        1 tgccggcctt gggggagaat tccgatgaca gttcagatgg ttttgctgcc cgtcttcgtt
       61 caggtgggcc tcactttggc gctgctgctg gcgatggcct cggcccggac cagggcgctg
      121 accgcgcggg aaaccagcct caaggacatc gctttggggc agccgaactg gccgacccgg
      181 gtcacccagt tcagcaactg ttacgccaac cagttcgaaa ttccgctgct gttttacatc
      241 ctgatcgcga ttgcgctgcc gctgcggcat gccgatctgg tgatcgtgct gctgtcctgg
      301 gtgttcgtgg tgacgcgctt cgcccatgcc ggaatttttg tcacctcgaa cgacgtccgc
      361 acccgcagcc ttgcatggtt cgccggcgtt ctggtggtgt tcgccatgtg ggtctggttc
      421 gcgctgaaga tcctgctctg aacgcgcgcc tcgttaccaa cgaaagcttt gaatgactcc
      481 tgctgccagg ctgtccgcgg ccattgaact gatcgagacc atcgacgcgc aacgcgtgcc
      541 ggcggcgaag gcgctcaagg agtggggcac cgcgcaccgt tatgccggct ccggcgaccg
      601 cgccgcgatc tccggcctga tctgggacgt gctgcggcgg cggtcgtcga gcgcctggat
      661 catggaagag gatacgccgc gcgcgcgcct gctcggcatg ctccggctcg agcgccagct
      721 ggatgcagac gccatctccg cgttgtgcga cggcggccgt ttcgcgccgg cgccgctcac
      781 cgacggcgag cgcgcggcac tgacgctgcg ctcgctggtg gatgcgcccg cgcacatcgc
      841 cggcgattat ccggaatggc tcgacggaca tctggcgcag gtgttcggcg atgaccgggt
      901 ggcggaggca accgcgatgg cgagccgggc gccgctcgat ttgcgcgtca acacgctgaa
      961 ggccaatcgc gaaaagatgc tgccgtcgct ggcccatctc ggcgcgcagc cgacgccgtg
     1021 gtcgccgctc ggcctgcgca tcgagctcgg cgccgacgcg cgcaatcccg gcattcacgc
     1081 ggagcaggat tttatcaagg gcgcgatcga agtacaggac gagggctcgc aactcgcgac
     1141 cctgttgtcg aatgcaaaac ccggcgagca ggtgatcgac ctctgcgccg gcgccggcgg
     1201 caagaccctg gcgctggcgg cgatgatgca gggcaagggc cggctgatcg cgaccgacca
     1261 cgacaagcgg cagctcgcac cgatctacga acggctgtcg cgcgccggcg cgcacaacgc
     1321 cgatgttcgc actccgaagg gaccgaacga tacgctcagc gatatccatg cctcggccga
     1381 tcttgtgctg atcgacgcgc cctgtaccgg caccggcacc tggcggcgca atcccgacgc
     1441 caagtggcgg atgcgcccag gcgcgctcga ggtgcggcta aaggatcagg tcaccgtgct
     1501 cgatcgtgcg gcggcgctgg taaaacccgg cgggcgcatc gcctacatta cctgctcggt
     1561 attgccgcag gaaaacggcg agcaggtccg cggttttgta gtccgccatc ccgagttttc
     1621 gataaccccg ccctcgcaga ccgtcggcgt gctgtgggac aaggccgagg atttcgcgag
     1681 cgccgcgctg gaatcggcgg agggctggct gatgacgccg cgccgcaccg gcacggatgg
     1741 gtttttcgtg tcggtgttga agaggaatta gccatgctcg caaagacgag gttaggcctc
     1801 gccaatacgc cagtccgggc tatgcagagc ccgccaagcc ggtggacggc cccgaatcga
     1861 cccgttttcg ggggttgcga gcctgccccc cgtcgcgtaa ttcctgtcca tgacagcagc
     1921 acagccagca gcagggtcga tgccttcggt ggcctcggcg catgacaaga ttctgatcgt
     1981 cgatttcggc agtcaggtaa cgcaactgat cgcgcgccgg gtgcgcgagg agggcgtcta
     2041 ttccgaaatc gtgccgttcc agaaggccga agccgccttc gcggcgatga agccgaaagc
     2101 ggtgatcctc tccggcggcc cggaatcggt gcatgaagca ggctcgccgc gcgcgccgca
     2161 ggcgatcttc gattcaggcg tgccggtgct cggcatctgt tacggccaga tggccatggc
     2221 cgcccagctc ggcggcgagg tcgagggcgg gcatcaccgc gagttcggcc gcgccgatgt
     2281 cgaggtgaaa gctgcgagca acctgttcga atcgacatgg tcgatgggcg aaaagcatca
     2341 ggtctggatg agtcatggcg accgcattac caagatgccg ccgggcttca cggtc
//
LOCUS       DATMJB010000644         3203 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_385169, whole genome shotgun sequence.
ACCESSION   DATMJB010000644 DATMJB010000000
VERSION     DATMJB010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385169"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="VNU48_13655"
     CDS             <1..1034
                     /locus_tag="VNU48_13655"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWI12373.1"
                     /translation="VDIAVLVILRLRRKKQAPEDQQRTRWVHWGCRVGLIAFIFADSN
                     MATNAWAPLWEQVCPSRVGALICGNGGPSETFLLVCFLLNGTVALPVFHAVRHHRVVD
                     VRFALSRGATLFVASFTIAALLAAVSIPIEHFLHESFATKVFVYVPVIAALKMVFDQL
                     HEWLKERCDRLFFKRLHMARERLEHVEARLIESKTRDAVDAQLVAQVVAALGLASAAV
                     FRRTSDGLYHRAAQSIGWPETSPPMPFRASLLRKLGQKQEPLRLTQFDMRTSMPQGSP
                     QPVIAMPVYDAGASGVIVLYGARTTGDNITDEEVDLLKTLSVAAGRAFERVEVLALRM
                     KIDELRANA"
     gene            1255..1494
                     /locus_tag="VNU48_13660"
     CDS             1255..1494
                     /locus_tag="VNU48_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12374.1"
                     /translation="MNTSPVSSEPADYELRYQSLFNAGRAYAFPCDAEGHVDIDALSD
                     HARGDYFYARALVGRELSMPSVIPSVARHRRVARP"
     gene            complement(1791..3110)
                     /locus_tag="VNU48_13665"
     CDS             complement(1791..3110)
                     /locus_tag="VNU48_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HWI12375.1"
                     /translation="MPKCTKDRIDFGRFGRRVIEADFSGGDLSSDGGLLLLRQVDRHL
                     GLSGMAAAAIPDPRDPERIRHGLRDLLAQRLYALCCGYEDLNDHKTLRDDVLMQTAVG
                     RDAALASAPTFSRLENRATRAQAWALHGVLIEQFIASHEEPPEELVLDIDASDVPLHG
                     EQELSQFHAYYDHHCYLPLYVFCGQAMLACYLRPSKIDGARHAAALIKLLVTRLRKVW
                     PATRFIVRADSGFCRRRLLHWCEHSGVDYIIGLARNARLHAAVEFAEASLADGYKASG
                     TKQRLIGEFSYAAKSWRHERRVITRLEYGDQGTNPRFIVTNLQGDAIQLYDRLYCQRG
                     EAENRIKEAQLDLFGTRASCSRFIANQFRLLLAALAYTLMQQLRALALQSTELERASA
                     ATIRVRLLKIGAAILRNTRRVRVMLASHHPLRELFATAAARLVALGP"
BASE COUNT          509 a         1055 c         1062 g          577 t
ORIGIN      
        1 gcgtcgatat cgcggtgttg gtcatcctgc gtctgcgccg aaagaagcag gcgcccgagg
       61 accagcaacg aacccgttgg gtgcactggg gctgccgggt ggggctgatt gccttcatct
      121 tcgccgacag caacatggcc accaacgcct gggcgccgct gtgggaacag gtctgcccgt
      181 cgcgcgtcgg cgcgctgatc tgcggaaatg gcggcccttc cgaaaccttc ctgctggtgt
      241 gtttcctgtt gaacgggacg gtggcgctcc cggtcttcca tgcggttcgc caccatcggg
      301 tcgtcgacgt gcgatttgcg ctgagccgcg gcgcgacact gttcgtggcc tcattcacca
      361 tcgcggcctt gctggccgcc gtcagcatac cgatcgaaca cttcctgcac gagtcctttg
      421 cgaccaaggt ctttgtctac gtgccggtca tcgccgccct gaagatggtc ttcgatcaat
      481 tgcacgagtg gttgaaggag cgctgcgacc gcctgttctt caaacggctg catatggcca
      541 gagagcggct cgaacatgtc gaggccaggc tgatcgagtc gaagactcgc gacgccgtcg
      601 acgcgcagct ggtcgcgcag gtcgtggcgg ctctcggcct cgcgtctgcc gccgtcttcc
      661 gacgcacttc cgatggcctc tatcaccggg cggcgcagtc gatcggttgg ccggaaacct
      721 cgccgcccat gccgttccgt gcgtcgctgc tgcgcaagct cgggcagaag caggagcccc
      781 ttcggttgac gcagttcgac atgcgcacgt cgatgccgca agggagcccc cagccggtga
      841 ttgcgatgcc ggtgtacgac gccggtgcat cgggcgtcat cgtgctttac ggcgctcgca
      901 cgaccggcga caacatcacc gacgaggagg tggatctgct gaagaccctg agtgtcgctg
      961 cggggcgtgc cttcgaacgc gtggaggtgc ttgcgctgcg catgaagatc gatgaattgc
     1021 gggcgaacgc ttgacgccgg agactcggcg atctcgagca agcggcggtc aacacacggg
     1081 tgcctcgctg cgttgagcag gtatccagac caacgaccgc cgaatcgccc cagcgccagg
     1141 agccgcgtca tcgttgcccg ttcacgcgcg cgacccctcg agtggggcat agccggcccg
     1201 cgcgatgctg cctacgctcg actcgacatg tcatctacgc caaagaggta cgccatgaac
     1261 acgtcccccg tgtcgagcga gcccgccgac tacgagttgc gctaccagtc gctgttcaat
     1321 gccgggcgcg cctacgcgtt cccctgcgac gccgaaggcc acgtggacat cgacgccctc
     1381 agcgaccatg cgcgcggaga ctatttctat gcacgcgccc tcgtcggccg cgagctgtcg
     1441 atgccgtcgg tgattccgag cgttgcgcgg cacaggcgtg tcgcgcgccc gtagccggtt
     1501 gttgccaggt ctggtgcgcc gcgatgaagc ccggtccatg tctggcggcc ggcacgctgg
     1561 cggcgctgct cgtcgcgatc ggcgcggcga acgccgccgc ggccgatctc ttcaactagc
     1621 ccggatattt caccagtagc tccgatcgca gcggcggggg gccggtcaag ctgccggtgg
     1681 tgtttgtgga cggcgcgtga ggtactggat gagcttgtgg ctgtttgcag gcgcaccgcc
     1741 cccttacccc cgttgtttgt caacgtgccg gggacagcac tggatgaggt ctacgggccg
     1801 agagcgacaa gccgagctgc cgcggtggcg aacaactcgc gcagcggatg gtggctggcc
     1861 agcatgaccc gcacgcggcg cgtgttgcgc aggatcgccg cgccgatctt gagcaagcgc
     1921 acgcggatcg tggctgcgct ggcacgctcc agctcggtgg actgcagtgc cagcgcgcgc
     1981 agctgctgca tcagcgtgta ggccaacgcc gccagcaaca gcctgaactg gttggcgatg
     2041 aagcgtgagc agctggcgcg ggtgccgaac agatcgagct gcgcttcctt gatgcggttc
     2101 tcggcctcgc cgcgctggca gtacagccgg tcgtacagct gtatggcatc gccctgcagg
     2161 ttggtgacga tgaagcgcgg gttggtgccc tgatccccgt attccagccg cgtgatgacg
     2221 cgccgctcat ggcgccagct cttggccgcg tagctgaact cgccgatgag tcgctgcttg
     2281 gtgccgctgg ccttgtagcc gtctgccagg ctcgcctcgg cgaactccac cgccgcgtgc
     2341 agccgtgcgt tgcgcgccag cccgatgatg tagtccacgc ccgagtgctc gcaccagtgc
     2401 agcaagcgtc gccggcagaa cccggagtct gcgcgcacga tgaagcgtgt ggccggccag
     2461 accttgcgca atcgtgtgac gagcagcttg atcagcgctg cggcgtgcct ggcaccatcg
     2521 atcttgctcg gtctcaagta gcacgccagc atcgcctggc cgcagaacac gtacagcggc
     2581 aggtagcagt gatggtcgta gtaggcgtgg aactgcgaga gttcctgctc gccgtgcagc
     2641 ggcacgtccg aggcatcgat gtccagcacc agttcctcgg gaggctcctc gtgggaagca
     2701 atgaactgct cgatgagcac accgtgcagt gcccacgcct gtgcgcgtgt agcccggttc
     2761 tccagccggc tgaacgtggg cgccgaggcc agcgccgcat cgcgcccgac cgcggtctgc
     2821 atcagcacat cgtcgcgcag cgtcttgtgg tcgttcaagt cctcgtagcc gcagcacagc
     2881 gcgtacaggc gctgcgccag cagatcgcgc aggccgtgtc ggatgcgctc gggatcgcgc
     2941 gggtcgggga tcgccgcggc cgccatgccg ctcaagccaa ggtggcgatc cacctggcgc
     3001 aacagcagca gcccgccgtc gctgctcaag tctccgccgc tgaagtcggc ctcgatcacg
     3061 cgccgcccaa agcggccgaa atcgattctg tcctttgtac actttggcat cggcagtcct
     3121 cggttgtcac aggcgtcaga tacctatgac aacgggctcg gccacgaggc tgccgacctc
     3181 gactgatgaa atatccgggc tag
//
LOCUS       DATMJB010000645         4136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_66801, whole genome shotgun sequence.
ACCESSION   DATMJB010000645 DATMJB010000000
VERSION     DATMJB010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66801"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VNU48_13670"
     CDS             complement(<1..552)
                     /locus_tag="VNU48_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWI12376.1"
                     /translation="MRRTFLIGFVVVLALLPQLPVPEFWITQANYIGLYTLVVTGLVL
                     LTGIAGLTSFGQAAFVGLGAYTAAYLTLTHGVSPWLTVWAGLALTFVAALVLGWITLR
                     MSGHYLPLATIAWGLSLYYLLGNIDALGKYDGLLGVPALKFFGIELNTGRSFFYLLWL
                     IVVLAALAATNLLDSRAGRALRAV"
     gene            complement(555..1595)
                     /locus_tag="VNU48_13675"
     CDS             complement(555..1595)
                     /locus_tag="VNU48_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWI12377.1"
                     /translation="MDFSIASILMLDGATNGAVYALLALATVLVFAITRVIFIPQGEF
                     VTFGALTLALLQMGKVPGTVWLLLALALLAAAQDIAGALKRGLPLDRTLRSAAATLVV
                     PIVVAALAWWAAPLKLPLVFQSLLTLAIVTPLGPLVYRLAYEKLADASVLVLLIVSVG
                     THFALTGLGLVFFGAEGYRNPSFWDARFSAGALSLSGQALIIFGVSIALILALYLFFE
                     RSLRGKALRATAVNRLGARLMGISTTGAGRLSFGLAAFMGALSGLLIGPTTTLFYDSG
                     FLIGLKGFVAAIFGGLASYPAAAVGALFVGLLESFGSFYASAFKEVIVFTVIIPVLLW
                     RSFQHGHDDEEE"
     gene            complement(1714..2859)
                     /locus_tag="VNU48_13680"
     CDS             complement(1714..2859)
                     /locus_tag="VNU48_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI12378.1"
                     /translation="MTLVRNLVAALAGLFAAGLAHADLTIGVSISLTGPTSALGIPTK
                     NGIELWPKTIAGEKVNLILLDDATDPTQGVKNTRRFVTEDKVDVIVGSVATPVAIAMA
                     DVAAEGQTVQLMLSPANLPEGKGAWSFRLPQSTGVMAIPIVEHWKKIGVKTYGFLGYA
                     DAYGEAWLKDIQPAAEKAGIKLIATERFARSDTSVTGQALKLVSANPDAILVVASGSG
                     AAMPHKGLVERGYAKAKIYQTHGAASRDLIRVGGADVEGSFVASGPAVVAPLLPDSNP
                     SKVQGVKYVTEYEKAFGKGTANQFGAHAFDVALVLEKAVPLALKKGKPGSKEFRVALK
                     DALETMGRTPVSQGVLNYTAADHFGFTPETGVLLTIAAGEWQVVPAK"
     gene            complement(2889..3323)
                     /locus_tag="VNU48_13685"
     CDS             complement(2889..3323)
                     /locus_tag="VNU48_13685"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWI12379.1"
                     /translation="MTRTTTTTVDVSFGDCDPAGIVFFPNFSRWMDTASLSFFMQCGV
                     PPWRELEKTRGIVGTPLLEINTKFLKPATYGETLTVTTQVEEWRAKVVVQLHRITRAR
                     ADGGVDLICEGREVRAFVKRDEHDRDRLRAIAVPDDIRRLCS"
     gene            complement(3352..4128)
                     /locus_tag="VNU48_13690"
     CDS             complement(3352..4128)
                     /locus_tag="VNU48_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI12380.1"
                     /translation="MKIEGQAAIVTGGASGLGAQTARELARRGAKVAVLDRHGAGARA
                     VAAEIGAYGQAMGIECDITSSDSVSAALAAARAAHGPARLLMNIAGIGTAKRLIGKDG
                     TPMPLEDFERVVRVNLIGTFNMIRLAAAEIVKLEPLEDGERGVIVNTASVAAFDGQVG
                     QEAYAASKGGLVALTLPLARDLAQFGVRVVTIAPGLFLTPLMAELPLPVQESLAASIP
                     FPKRLGKPEEFAQLCAAIVENLALNGEVIRLDGALRMAPR"
BASE COUNT          671 a         1420 c         1457 g          588 t
ORIGIN      
        1 gacggcgcgg agggcccggc cggcgcgcga atcgagcaga ttggtcgcgg ccagcgcggc
       61 cagcacgacg atcagccaca gcaggtagaa gaagctgcgc ccggtgttga gttcgatgcc
      121 gaagaacttc agcgccggca cgccgagcag gccgtcgtat ttgccgagtg cgtcgatgtt
      181 gccgagcagg tagtacaggc tcaggcccca cgcgatggtc gcgagcggca ggtagtggcc
      241 cgacatgcgc aaggtgatcc agcccagcac cagcgcggcc acgaaggtga gcgcgagccc
      301 ggcccagacg gtgagccagg gcgagacgcc gtgggtcagc gtcaggtagg ccgcggtgta
      361 ggcgccgagg ccgacgaacg cggcctggcc gaacgaggtg agcccggcga tgccggtcag
      421 cagcaccagg ccggtcacga ccagcgtgta caggccgatg tagttggcct gggtgatcca
      481 gaactcgggc accggcagct gcggcagcag cgccagcaca accacgaagc caatcaggaa
      541 tgtgcgacgc atgctcactc ttcttcgtcg tcgtggccgt gctggaacga gcgccacagc
      601 agcaccggga tgatcacggt gaacacgatc acctccttga acgcactcgc gtagaacgag
      661 ccgaagcttt cgagcaggcc gacgaacagc gcgccgaccg ccgccgccgg gtagctcgcc
      721 aggccgccga agatcgccgc gacgaagccc ttcaggccga tcaggaaacc cgagtcgtag
      781 aacagcgtcg tcgtcggccc gatcagcagg cccgacagcg cgcccatgaa ggccgcgagg
      841 ccgaagctga gccgcccggc gccggtcgtc gagatgccca tcaggcgcgc gccgagccgg
      901 ttcaccgcgg tcgcgcgcag cgccttgccg cgcaacgacc gctcgaagaa caggtacagc
      961 gcgaggatca gcgcgatcga gaccccgaag atgatcaggg cctggcccga caggctgagt
     1021 gcgccggcgc tgaagcgcgc gtcccagaag ctcgggttgc ggtaaccctc ggcgccgaag
     1081 aacaccaggc cgagaccggt caacgcgaag tgcgtgccga ccgagacgat cagcagcacc
     1141 agcacgctcg cgtcggcgag tttctcgtag gccaggcgat agaccagcgg gcccagcggc
     1201 gtgacgatcg ccagcgtcag caaactctgg aacaccagcg gcagcttcag cggcgcggcc
     1261 caccaggcga gcgcggcgac gacgatcggg accaccagcg tcgcggcggc cgagcgcagc
     1321 gtgcggtcca gcggcaggcc gcgcttcagc gcgccggcga tgtcctgcgc cgcggcgagc
     1381 agcgcgagcg cgagcagcag ccagaccgtg cccggcacct tgcccatctg cagcagcgcc
     1441 agcgtgagcg caccgaaggt cacgaactcg ccctgcggga tgaagatcac ccgcgtgatc
     1501 gcgaacacca gcaccgtggc cagcgcgagc agcgcataga cggcgccgtt ggtggcgcca
     1561 tcgagcatca ggatgctggc gatcgaaaag tccatgggcg acacgccggg ttgaagcgaa
     1621 acttactcga ccgaccggtc ggtcaattcg gcacgggatc atacaagcgc cagacgcgcc
     1681 gccgcgcgcg gcgcgccccg gacaaaccct tggctacttg gccggcacca cctgccactc
     1741 gccggcggcg atcgtcagca gcacaccggt ctcgggcgtg aagccgaagt ggtcggcagc
     1801 ggtgtagttc agcacgccct gcgagaccgg cgtgcggccc atggtctcga gcgcgtcctt
     1861 cagcgcgacg cggaactcct tgctgccggg cttgcccttc ttcagcgcca gcggcaccgc
     1921 cttctcgagc accagcgcga cgtcgaacgc gtgcgcgccg aactggttcg cggtgccctt
     1981 gccgaaggcc ttctcgtact cggtgacgta cttgacgccc tgcaccttgc tcgggttgct
     2041 gtcgggcagc agcggcgcca ccacggccgg cccggacgcg acgaacgagc cttcgacgtc
     2101 ggcaccgccg acgcggatca ggtcgcggct ggccgcgccg tgggtctggt agatcttggc
     2161 cttcgcgtag ccgcgctcga ccaggccctt gtgcggcatc gccgcgccgc tgcccgaggc
     2221 cacgaccagg atcgcgtcgg ggttggccga gacgagcttg agcgcctggc cggtcacgct
     2281 ggtgtcggag cgtgcgaagc gctcggtcgc gatcagcttg atgccggcct tctcggcggc
     2341 cggctggatg tccttcagcc aggcctcgcc gtaggcatcg gcgtagccca ggaagccgta
     2401 ggtcttgacg ccgatcttct tccagtgctc gacgatcggg atcgccatca cgccggtcga
     2461 ttgcggcagc cggaacgacc aggcgccctt gccctcgggc aggttcgccg gcgacagcat
     2521 cagctgcacc gtctggcctt cggcggcgac atccgccatc gcgatcgcga ccggcgtggc
     2581 caccgagccg acgatcacgt cgaccttgtc ctcggtgacg aagcggcgcg tgttcttgac
     2641 gccctgggtc gggtcggtcg cgtcgtcgag caggatgagg ttgaccttct cgccggcgat
     2701 cgtcttcggc cacagctcga tgccgttctt ggtcgggatg cccagcgccg aggtcggccc
     2761 ggtcagcgag atgctgacgc cgatcgtcag atcggcgtgc gccaggccgg cggcgaacag
     2821 gccggccagg gcggccacga ggttgcggac gagggtcatg gaagtctcct tctgtggtgt
     2881 ttcgagggtc aggaacagag ccggcggatg tcgtcgggca cggcgatcgc gcgcaggcgg
     2941 tcgcggtcgt gctcgtcgcg cttgacgaag gcgcgcacct cgcggccctc gcagatcagg
     3001 tcgacgccgc cgtcggcgcg cgcgcgggtg atgcggtgca gctgcacgac gaccttggcg
     3061 cgccattcct cgacctgcgt cgtcacggtc agcgtctcgc cgtaggtggc cggcttgagg
     3121 aacttggtgt tgatctcgag cagcggcgtg ccgacgatgc cgcgcgtctt ctcgagctcg
     3181 cgccacggcg gcacgccgca ctgcatgaag aacgacagcg aggcggtgtc catccagcgc
     3241 gagaagttcg ggaagaacac gatccccgcc gggtcgcagt cgccaaagct cacgtcgacg
     3301 gtggtggtgg tcgtgcgcgt catgtggggt tcggtgcggg cagtaactca gtcagcgcgg
     3361 cgccatgcgc agcgcgccgt ccaggcggat cacctcgccg ttgagcgcga ggttctcgac
     3421 gatagccgcg cacagctgcg cgaactcctc gggtttgccc agccgcttcg ggaacgggat
     3481 gctggccgca agcgactcct gcaccggcag cggcagctcg gccatcagcg gcgtcaggaa
     3541 caggcccggc gcgatcgtca cgacgcgcac gccaaactgc gcgaggtcgc gcgcgagcgg
     3601 cagagtcagc gcgaccaggc cgcccttcga cgcggcatac gcctcctgcc cgacctggcc
     3661 atcgaacgcc gcgaccgacg cggtgttgac gatcacgcca cgctcgccgt cttccagcgg
     3721 ctcgagcttg acgatctcgg cggcggccag gcggatcatg ttgaaggtgc cgatcaggtt
     3781 gacgcgcacg acgcgctcga agtcctcgag cggcatcggc gtgccgtcct tgccgatcag
     3841 gcgtttcgcg gtgccgatgc cggcgatgtt catcagcagc cgcgccggcc cgtgcgccgc
     3901 gcgcgcggcc gcgagcgcgg cgctgacgct gtcgctggag gtgatgtcgc attcgatgcc
     3961 catcgcttgt ccgtacgcgc cgatctcggc ggcgaccgcg cgcgcgccgg cgccgtggcg
     4021 gtcgagcacc gcgaccttcg cgccgcgccg cgcgagctcg cgcgccgttt gcgcgccgag
     4081 gccggaggcg ccaccggtga cgatggcggc ttgtccttcg atcttcattc gtgtat
//
LOCUS       DATMJB010000646         2411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_88939, whole genome shotgun sequence.
ACCESSION   DATMJB010000646 DATMJB010000000
VERSION     DATMJB010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88939"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VNU48_13695"
     CDS             complement(<1..428)
                     /locus_tag="VNU48_13695"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWI12381.1"
                     /translation="MNHPNALKRLFYPESIALVGASERSPWSQLLHANIQRVGYTGRI
                     CAVNKTGAAAHGYPGYRACREIPEAVDAAYLFVPLDAVLDAFDDVVDSGIKSAVILSS
                     GFAEAGPEGAALQDELTRRARAAGVTFLGPNCLGFANLAI"
     gene            complement(469..2142)
                     /locus_tag="VNU48_13700"
     CDS             complement(469..2142)
                     /locus_tag="VNU48_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HWI12382.1"
                     /translation="MTLFDLIIVGAGSAGCVLANRLSADGRRVLLIEAGGDDASPLIR
                     MPKGMAKLVADPRHAWHFPVAQRREPGLPAAEVWVRGKGLGGSSSINGMIYTRGQPQD
                     YDAWGEVAGSDWNWAAMKAAFMAIENHELGPDEQRGAGGPLHISTNRFRYRLSEAAVR
                     AGEELGLPRREDLNREDQEGVGYYAHTIHRGRRFSAADAFLEPALKRPNLRVVTGTHV
                     ERVLFDGRRAVGVAARNAQGAVDYRCSGEVILCAGTLLSPKILQLSGVGPGALLQSLG
                     IAVVRDSPDVGARLRDHLGLLSSHRLVGDKGIGHCFYGIGLAKSVLQYVLLKSGPMAT
                     GPFEVGAFARTEPQLALPDTQLYISAFTYPRSDDNFPVPGGIERKPGVTIYAQMLKLS
                     SEGRIRVSSADPQAPLDIEPNWLSNAADRHTAVATLKLMRRYMAQPALAPYLGEELVP
                     GAQCADDEDLLRYARRAALCGLHAVGSCRMGRDDRAVVDERLRVRGVDGLRVVDCSVM
                     PGLVSANTNGPAMALGWRAADLILEDLRAAAGGADPARLAGVGCAACLP"
     gene            complement(2139..>2411)
                     /locus_tag="VNU48_13705"
     CDS             complement(2139..>2411)
                     /locus_tag="VNU48_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin"
                     /protein_id="HWI12383.1"
                     /translation="PAADCHADEHAAVLRSAHEVLARVDAGDVAIGRAFAAELARWFP
                     SHVAHLDSALAHWLVKKRFGAAPLVLRRQRSLTSPEVTRAGERVGP"
BASE COUNT          367 a          844 c          855 g          345 t
ORIGIN      
        1 tggatcgcca ggttggcgaa gcccaggcag ttggggccga ggaaggtgac gcccgcggcg
       61 cgcgcgcgcc gcgtcagctc gtcctgcagc gccgcgcctt cggggccggc ttctgcaaag
      121 ccggagctca ggatcactgc cgacttgatg ccgctgtcaa cgacgtcgtc gaacgcatcg
      181 agcaccgcgt cgagcggcac gaacaggtag gcggcatcca ccgcttccgg aatctcgcgg
      241 caggcccgat agcccggata gccgtgcgcg gcggcgccgg tcttgttcac cgcgcagatg
      301 cgcccggtgt agccgacccg ctggatgttg gcatgcagca gctgcgacca cggcgagcgc
      361 tccgaggcgc cgaccagcgc gatcgattcc ggatagaaca agcgcttgag cgcattgggg
      421 tggttcatgt ctctccttgt cgggcccgac cgtgccacgg cctgcgggtc acgggaggca
      481 cgccgcgcaa ccgacgccag ccaaacgcgc cggatcggct ccgccggcgg ccgcgcggag
      541 gtcttccagt atcaggtcgg cggcgcgcca gcccagggcc atcgccgggc cgttggtgtt
      601 ggcggacacc aggccgggca tgaccgagca atcgaccacg cgcaggccgt cgacgccgcg
      661 cacgcgcagg cgttcgtcga cgacggcgcg atcgtcgcga cccatgcggc aggaaccgac
      721 cgcatgcagc ccgcacagcg cggcgcggcg tgcatagcgc agcaggtctt cgtcgtcggc
      781 gcactgcgcg cccggcacca gttcctcgcc gagatagggc gcgagcgccg gctgcgccat
      841 gtagcggcgc atcagcttca atgtcgccac ggcggtatgc cgatcggcgg cgttcgacag
      901 ccagttgggc tcgatgtcca gcggcgcctg cgggtcggcc gagctgacgc ggatgcggcc
      961 ttcgctgctg agcttgagca tctgcgcata gatcgtgacg ccaggctttc gttcgatgcc
     1021 gccgggcacc gggaagttgt cgtcgctgcg tggataggtg aaggcgctga tgtagagctg
     1081 cgtgtcgggc agcgcgagct gcggctcggt gcgggcgaag gcgcccacct cgaacggccc
     1141 ggtcgccatc ggcccacttt tgagcaggac gtactgaagc acgctcttgg ccaggccgat
     1201 gccatagaaa caatggccga tgcccttgtc cccgaccagc cggtggctgc tcagcaggcc
     1261 caggtggtcg cgcaggcgcg cgccgacgtc ggggctgtcg cggacgaccg cgatgcccag
     1321 cgattgcagc agcgcgcccg gcccgacgcc ggagagctgc aggatcttcg gtgacagcag
     1381 cgtgccggcg cagaggatga cttcgccgct gcagcgatag tccaccgcgc cctgcgcatt
     1441 gcgggcggcg acgccgaccg cgcgtcggcc gtcgaacagc acgcgctcga catgggtgcc
     1501 ggtgacgacg cgcagattgg gccgcttcag cgccggctcg aggaaggcat cggcggcgct
     1561 gaaacggcga ccccgatgga tggtgtgggc gtagtagccc acgccttcct ggtcttcgcg
     1621 gttgaggtcc tcgcggcgcg gcaggccgag ctcctcgccg gcgcgcaccg ccgcttcgct
     1681 cagccggtag cggaacctgt tggtgctgat gtgcagcggc ccgcccgcgc cgcgctgctc
     1741 atcgggcccc agttcatggt tctcgatggc catgaaggcg gccttcatgg ccgcccagtt
     1801 ccagtccgat cccgcaacct cgccccacgc gtcgtagtcc tgcggctggc ctcgggtgta
     1861 gatcatgccg ttgatcgaac tcgacccacc caggcccttg ccgcggaccc acacttcggc
     1921 cgccggcaag cccggctcgc gccgctgcgc gaccgggaaa tgccaggcgt ggcgtggatc
     1981 cgccaccagc ttggccatgc ccttgggcat tcggatcaac gggctggcgt cgtcgccacc
     2041 ggcctcgatc agcaggacgc ggcggccatc cgccgacaag cggttggcaa gcacgcagcc
     2101 cgccgaaccg gcgccgacga tgatgaggtc aaagagggtc atggacccac ccgctcgcca
     2161 gcgcgagtga cctcgggcga tgtcagcgag cgttggcgcc gcaagaccaa gggcgcagcg
     2221 ccgaagcgct ttttgaccag ccaatgggcg agtgccgaat ccaggtgcgc cacatgggac
     2281 gggaaccagc gcgccagctc ggccgcgaac gcgcggccga tcgccacgtc gccggcgtcg
     2341 acgcgcgcca gcacctcgtg cgccgagcgc agcaccgccg catgttcgtc ggcatggcag
     2401 tcggcggccg g
//
LOCUS       DATMJB010000647         2661 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_30243, whole genome shotgun sequence.
ACCESSION   DATMJB010000647 DATMJB010000000
VERSION     DATMJB010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30243"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1854)
                     /gene="dsbD"
                     /locus_tag="VNU48_13710"
     CDS             complement(22..1854)
                     /gene="dsbD"
                     /locus_tag="VNU48_13710"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HWI12384.1"
                     /translation="MRPRDLLNLCLQLCATVLLLTGAPARAEDDFLDPEVAFKLAARA
                     ADERTIELTYTIAPGYYLYREQFKFAATGATLGAPVLPVGKTKFDETFQKTVEIYRDV
                     VRITIPVQQASPGFRLLATNQGCADKGLCYPPQQRGVEFKLAGFGGDGSARVLSASEN
                     PIVAAAVTPGATPPAATTYAADDAGIDSALRSGKLWPVIGVFFLGGVLLSLTPCMLPM
                     LPILSSIIVGHGAQVSRGRGLALAASYSLGMALVYTALGVAAGLAGEGLAATLQKPWV
                     LALFAAGLVALSLSMFGVYELQLPAAITGRLSGASQRLPAGRFAGVFAMGGVSALIVS
                     PCVAAPLAGALVYLSQTRDVWLGGTALFSLAAGMSVPLLLMGASAGALLPRAGGWMDD
                     VKRFFGLMLLGVALWTVQSVLAAPLVLALWGALAIGAAVMLASRRDGRHSGWTRSLWR
                     RAAAALLLVGGLLQLVGAASGGSNPLQPLAHLRAGAEAAAQHGPVFTTVRSSADLDAA
                     LRGAGRPVMLDFYADWCVSCKEMERFTFVDPAVQKKMSGALLLRADVTANNADDRELL
                     KRFQLFGPPGTIFFDASGRELPAARIAGFQDSARFLQTLQTAGL"
     gene            complement(1995..2387)
                     /gene="rplQ"
                     /locus_tag="VNU48_13715"
     CDS             complement(1995..2387)
                     /gene="rplQ"
                     /locus_tag="VNU48_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HWI12385.1"
                     /translation="MRHRHGLRKLNRTSEHRLAMLRNMCNSLLQHEAIKTTVPKAKEL
                     RRVVEPLITLAKDATLANRRLAFDRLRDRDIVTKLFNELGPRYKARPGGYTRILKMGF
                     RVGDNAPMAFVELVDRPEPVAADAKADE"
     gene            complement(2522..>2661)
                     /locus_tag="VNU48_13720"
     CDS             complement(2522..>2661)
                     /locus_tag="VNU48_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HWI12386.1"
                     /translation="TETELLKTPNLGRKSLNEIKEVLASRGLTLGARLENWPPQGLDK
                     R"
BASE COUNT          452 a          908 c          908 g          393 t
ORIGIN      
        1 aacgggggtt ttccggcgcg gtcacagacc ggcggtctgc aaggtctgca ggaaccgcgc
       61 actgtcctgg aagcccgcga tgcgcgcggc gggcagttcg cggccgctgg cgtcgaagaa
      121 gatcgtgccc ggtgggccga acagctggaa gcgcttcaac agctcgcgat cgtcggcatt
      181 gttggcggtc acgtcggccc tcagcagcag cgcgccgctc atcttcttct gcaccgccgg
      241 gtcgacgaac gtgaagcgct ccatctcctt gcacgagacg caccagtcgg cgtagaaatc
      301 cagcatcacc ggccggccgg cgccgcgcag cgccgcatcg agatcggcgc tgctgcgcac
      361 cgtcgtgaac accgggccgt gctgcgcggc ggcctcggcg cccgcgcgca gatgggcgag
      421 cggttgcagc gggttcgaac cgccggacgc ggcccccacg agctgcagca ggccgccgac
      481 aagaagcagc gccgcggcgg cgcggcgcca caggctgcgc gtccagccgg aatgccgacc
      541 gtcgcgccgt gaagccaaca tgaccgcggc gccgatcgcc agcgcgcccc acagcgcaag
      601 caccaacggg gcggccagca ccgactgcac ggtccacagg gcgacgccga gcagcatcag
      661 gccgaagaag cgcttgacat cgtccatcca tccgccggcg cgcggcagca gggcaccggc
      721 cgacgcgccc atcagcaaca gcggcacgct catgcccgcg gcgagcgaga acagcgccgt
      781 gccgccgagc cagacatcgc gtgtctggct caggtagacc agcgcgcccg ccagcggcgc
      841 ggccacgcag gggctcacga tcagggccga aacaccaccc atcgcgaaga cgccggcaaa
      901 gcgcccggcg ggaaggcgct gcgacgcacc ggacaggcgc ccggtgatcg ccgcaggcag
      961 ctgcaattcg tagacgccga acatcgacag cgacaacgcg accagcccgg ccgcgaacag
     1021 cgccagcacc cacggcttct gcagcgttgc ggcgaggccc tcgccggcca gccctgccgc
     1081 gacaccgagc gcggtgtaaa ccagcgccat gcccagcgag tagctggccg ccagcgccag
     1141 cccgcgacca cgcgagacct gtgcgccatg gccgacgatg atggacgaca ggatcggcag
     1201 cattggcagc atgcagggcg tcagcgagag cagaacccca ccgaggaaga acaccccgat
     1261 caccggccac agcttgccgc tgcgcagggc gctgtcgatg ccggcgtcat cggcagcata
     1321 ggtggtcgcc gccggcggtg ttgcgccagg cgtcacggca gctgcgacga tcggattttc
     1381 gctggccgac agcacgcgcg ccgagccatc cccgccgaag ccggccaact tgaactcgac
     1441 gccgcgctgc tgcggcgggt agcacaggcc cttgtctgca cagccctggt tggtcgccag
     1501 caagcggaac ccgggcgacg cctgctgcac cgggatcgtg atgcgcacca cgtcgcggta
     1561 gatctcgaca gtcttctgga atgtttcgtc gaacttggtc ttgccgaccg gcaacaccgg
     1621 cgcccccagg gtcgcgccgg tcgccgcgaa cttgaactgc tcgcggtaga ggtagtagcc
     1681 cggcgcgatc gtgtacgtga gctcgatggt gcgctcgtcg gccgcgcgcg cggcgagttt
     1741 gaacgcgacc tccgggtcga gaaaatcgtc ctcggcacgc gccggtgcgc ccgtgagcag
     1801 cagcacggtc gcgcacaact gcaggcacag gttcagaagg tctcgggggc gcatgaggat
     1861 ggggcgaagc agctgattcg aatgaccccg gtccgcgacc gaggttcccg cattcgatgg
     1921 tagccaattg cgcgcccgat cggctcgagc gaaaaaggcc ggcaatgccg gccttcctgt
     1981 cgcgcagcga cgctttactc gtcagccttg gcgtcggccg ccacgggttc ggggcgatcg
     2041 acgagttcga cgaaggccat cggcgcgttg tcgccgacgc ggaagcccat cttcaagatg
     2101 cgcgtgtagc cgcccgggcg cgccttgtag cgcgggccga gttcgttgaa cagcttggtg
     2161 acgatgtcgc ggtcgcgcag gcgatcgaac gcgagccggc ggttcgccag cgtggcgtcc
     2221 ttggcgaggg tgatcagcgg ctcgacgacg cgacgcagtt ccttggcctt cgggacggtg
     2281 gtcttgatcg cctcgtgttg cagcagcgaa ttgcacatgt tgcgcagcat cgccaggcga
     2341 tgctcgctgg tgcggttgag ttttctgagt ccgtggcggt gacgcatggt gcttttcctt
     2401 tctcactaac ttattgaacc ccagccgtat caggtactgg gggttgcacg atcaaacacg
     2461 cctgagcgtg tctgtcatcc gatgacggct tcagccgtct ttcctgttgg agcgaagcgg
     2521 tctatcgttt gtcgagaccc tgcggcggcc agttctcgag gcgggcgccg agcgtgagcc
     2581 cgcgcgatgc cagcacttcc ttgatctcgt tgagcgactt gcgacccagg ttcggggtct
     2641 tcagcaactc ggtctcggtg c
//
LOCUS       DATMJB010000648         2293 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_680288, whole genome shotgun sequence.
ACCESSION   DATMJB010000648 DATMJB010000000
VERSION     DATMJB010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680288"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="VNU48_13725"
     CDS             complement(<1..196)
                     /locus_tag="VNU48_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HWI12387.1"
                     /translation="MTHRDTPPFRADHVGSFLRPKFLLDAREQHARGEITPEQLRAVE
                     DRAITEIVEFQEDVGLQSITD"
     gene            290..1480
                     /locus_tag="VNU48_13730"
     CDS             290..1480
                     /locus_tag="VNU48_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI12388.1"
                     /translation="MTTKRRALKLALALLTSAAAGACAAAAANEGGPIKIGLILPMTG
                     QSASTGRQIDAAVKLWMAQNGNKIAGRSVEVILKDDGAVPDATKRIAQELIVNDKVTV
                     IAGFGITPTALATAPLATQSRTPLVVMAAATSSITQASPYIVRTSFTLPQVTVAIADW
                     AAKNKIKKAVTLVADYGPGFDAEKFFDSQFRLDGGQVLDKLRTPLRAPDFAPVLQKVI
                     DVKPDALFVFLPSGQGAAFMKQFAERGLDKSGVRLIATGDVTDDDQLNDMGDVAIGVV
                     NSHHYSAAHPSAANKKFVAAFEAANKFRPNFMAVGGYDGMRVIYKALEATKGAGGEAL
                     LEAMKGQVFESPRGPVYIDGQTRDIVQDVYIRKVERVPAMNNGLYNVEFDVIKAVKDP
                     GKAK"
     gene            1517..>2293
                     /locus_tag="VNU48_13735"
     CDS             1517..>2293
                     /locus_tag="VNU48_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWI12389.1"
                     /translation="MLTLLFDGIAYGMLLFVLAVGLAVTLGLMNFINLAHGAFAMAGG
                     YITVLLMNRFGVPFLVCLPLAFLGAALLGALLEPLLYRRMYAKPHLDQVLFSIGLVFI
                     AITAVDYVVGSMQQNITLPGWLQGRFDIEWIGVGKYRLFIIVVCAALTLALQAVLTRT
                     RFGAQLRAAVDDPRVAGGLGLNVNRIFLLTFAVGSGLAGLGGALGAEVLGLDPTFPLK
                     FMVYFLIVCAVGGTTTITGPLLAALLLGIADVAGKYYVPKL"
BASE COUNT          354 a          778 c          775 g          386 t
ORIGIN      
        1 cgtcggtgat gctttgcagg cccacgtctt cctggaattc gacgatctcg gtgatcgccc
       61 ggtcctcgac cgcgcgcagc tgctcgggtg tgatctcgcc cctcgcgtgc tgctcgcgcg
      121 cgtcgagcag gaacttcggg cgcaggaagc tgccgacgtg gtcggcgcgg aacggtggtg
      181 tgtcgcggtg cgtcatcgtg cctctccgta cgggggtttg aaccatgata gccagcgtcg
      241 acagttcgct acagttggcc cggccagttc atgccttccg gagacaccca tgaccaccaa
      301 acgtcgtgcc ctgaaactcg cgctcgcgct cttgacgagc gcagcggcgg gcgcctgcgc
      361 cgccgcggcc gccaacgagg gcggcccgat caagatcggc ctgatcctgc cgatgaccgg
      421 ccagtcggca tcgaccgggc gccagatcga cgccgcggtc aagctgtgga tggcgcagaa
      481 cggcaacaag atcgccggcc gcagcgtcga ggtgatcctg aaggacgacg gcgccgtgcc
      541 cgacgcgacc aagcgcatcg cgcaggagct gatcgtcaac gacaaggtca ccgtgatcgc
      601 cggcttcggc atcacgccga ccgcgctggc gaccgcgccg ctggccacgc agagcaggac
      661 gccgctggtc gtgatggccg cggccaccag cagcatcacg caggcctcgc cgtacatcgt
      721 gcgcacgagt ttcaccttgc cgcaggtcac ggtggcgatc gccgactggg ccgcgaagaa
      781 caagatcaag aaggccgtca cgctggtggc cgactacggc ccgggcttcg acgccgagaa
      841 gttcttcgac agccagttcc gcctcgacgg cggccaggtc ctcgacaagc tgcgcacgcc
      901 tttgcgcgcg cccgacttcg ccccggtgct gcagaaggtg atcgacgtga agcccgatgc
      961 gctgttcgtg tttctgccgt cgggccaggg cgcggcgttc atgaagcagt tcgccgagcg
     1021 cggtctggac aagtccggcg tgcgcctgat cgccaccggc gacgtgaccg acgacgacca
     1081 gctgaacgac atgggcgacg tggcgatcgg cgtcgtcaat tcgcaccact actcggcggc
     1141 gcacccgtcg gccgcgaaca agaagttcgt cgccgcgttc gaggccgcga acaagttccg
     1201 cccgaacttc atggcggtgg gcggctacga cggcatgcgc gtgatctaca aggcgctcga
     1261 ggcgaccaag ggcgcgggcg gcgaggcgct gctcgaggcg atgaagggcc aggtcttcga
     1321 gagcccgcgc ggcccggtct acatcgacgg ccagacgcgc gacatcgtgc aggacgtcta
     1381 catccgcaag gtcgagcgcg tgccggcgat gaacaacggg ctgtacaacg tcgagttcga
     1441 tgtcatcaag gccgtcaagg acccgggcaa agccaagtag gcgggatggc ctgaacgcgg
     1501 ccgtcgaact gcatccatgc tgacactgct cttcgacggc atcgcctacg gcatgctgct
     1561 gttcgtgctc gcggtcgggc tggccgtgac gctgggcctg atgaacttca tcaacctcgc
     1621 gcacggcgcg ttcgcgatgg cgggcggcta catcaccgtg ctgctgatga accggttcgg
     1681 cgtgccgttc ctggtgtgcc tgccgctcgc gttcctcggt gccgcgctgc tgggcgcgct
     1741 gctcgagccg ctgctgtacc ggcgcatgta cgccaagccg cacctggacc aggtgctgtt
     1801 ctcgatcggc ctggtgttca tcgccatcac cgcggtcgac tacgtcgtcg gctcgatgca
     1861 gcagaacatc acgctgcccg gctggctgca gggccgcttc gacatcgagt ggatcggtgt
     1921 cggcaagtac cggctgttca tcatcgtcgt ctgcgcggcg ctgacgctgg cgctgcaggc
     1981 ggtgctgacg cgcacgcgct tcggcgcaca gttgcgcgcg gccgtcgacg acccgcgcgt
     2041 cgcgggcggg ctcggtctca acgtcaaccg catcttcctg ctgaccttcg cggtcggctc
     2101 cggtctggcc gggctgggcg gcgcgctcgg cgccgaagtg ctcggcctgg acccgacctt
     2161 cccgctgaag ttcatggtct atttcctgat cgtctgcgcg gtcggcggca cgaccacgat
     2221 caccggcccg ctgctggccg cgctgctgct cggcattgcc gacgtcgccg gcaagtatta
     2281 cgtgcccaag ctc
//
LOCUS       DATMJB010000649         2607 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_29941, whole genome shotgun sequence.
ACCESSION   DATMJB010000649 DATMJB010000000
VERSION     DATMJB010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29941"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="VNU48_13740"
     CDS             complement(<1..175)
                     /locus_tag="VNU48_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HWI12390.1"
                     /translation="MHDIIQQLEAKRVKARLGGGEKRIAAQHGKGKLTARERIELLLD
                     ADTFEEWDMFVEHR"
     gene            complement(184..1152)
                     /gene="meaB"
                     /locus_tag="VNU48_13745"
     CDS             complement(184..1152)
                     /gene="meaB"
                     /locus_tag="VNU48_13745"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HWI12391.1"
                     /translation="MNSVDEALVSGVRAGDRRALAKSITLLESTRADHRARADELLNS
                     LLPHTGHGMRLGISGVPGVGKSTFIETLGLTLIAKGHRVAVLAIDPSSSLSGGSILGD
                     KTRMERLSVDEAAYIRPSPASGTLGGVAEKTREAMLVCEAAGFDVVIVETVGVGQSET
                     TVASMTDLFVLLQLPNAGDDLQAIKKGVMELADLIVINKADLDPLAATRAQAQITSSL
                     RLFAGHDSMPRVLTMSALKSDGFEAFWAAVQEFHAQRRASGAFAARRKEQSLAWMWDI
                     IQARLQADFRHHAAVREALPRAVQGVTAARIAPSVAARTLLNLFER"
     gene            complement(1163..>2607)
                     /locus_tag="VNU48_13750"
     CDS             complement(1163..>2607)
                     /locus_tag="VNU48_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWI12392.1"
                     /translation="MQEAGANQALELAFTLADGREYVKAALAKGLDVDEFAGRLSFFW
                     AIGMNFYLEVAKMRAARLLWWKIMKGFDAKNPKSLMLRTHCQTSGWSLTEQDPYNNVV
                     RTTIEAMAAVFGGTQSLHTNALDEAIALPTEFSARIARNTQLIIQEETHITSVVDPWA
                     GSYMMEKLTQDMADAAWKIVEEVEAMGGMVKAVDSGWAKLKIEASAAEKQARIDSGKD
                     VIVGVNKYRLGTEDAIETRDIDNAMVRDNQVARLRQLRAAREGAKTQAALDALTRCAE
                     TGEGNLLDLSIKAVRLRATVGEVSDALEKAWGRHRADTQKVTGVYAAAYDSAEGWDKL
                     KTEIAQFAEDQGRRPRVMIAKLGQDGHDRGAKVVATAFADLGFDVDMGPLFQTADECA
                     RQAIENDVHAVGVSTLAAGHKTLVPAIIESLRQQGADDIIVFVGGVIPRQDYEMLFDA
                     GVKGIYGPGTPIPASAKDVLEQIRMSVAKG"
BASE COUNT          360 a          930 c          843 g          474 t
ORIGIN      
        1 tgcggtgctc gacgaacatg tcccactctt cgaacgtgtc cgcgtcgagc agcagctcga
       61 tgcgttcgcg cgccgtgagc ttgcccttgc cgtgttgcgc cgcgatgcgc ttctcgccgc
      121 cgcccaggcg cgccttcacg cgcttggctt cgagctgctg gatgatgtcg tgcatggtct
      181 ggattacctc tcgaatagat tcagcaaggt gcgcgcggcg accgaagggg cgatgcgcgc
      241 cgcggtcacg ccctgaacgg cccggggcag tgcttcgcgc accgccgcat ggtggcggaa
      301 gtcggcctgc aagcgggcct ggatgatgtc ccacatccac gccagcgact gctccttgcg
      361 gcgcgccgcg aacgcgccac tcgcgcgtcg ttgcgcgtgg aattcctgga cggcggccca
      421 gaacgcctcg aagccatcgc tcttcagcgc actcatcgtc agcacgcgcg gcatgctgtc
      481 gtggccggcg aacagccgca gcgaactcgt gatctgggcc tgcgcgcgcg tcgccgccag
      541 cgggtcgaga tcggccttgt tgatgacgat cagatcggcc agttccatca cgcccttctt
      601 gatcgcctgc aggtcgtcgc cggcgttggg cagctgcagc agcacgaaca ggtcggtcat
      661 gctcgccacc gtggtctcgc tctgcccgac gccgacggtc tcgacgatca cgacgtcgaa
      721 gcccgcggcc tcgcacacca gcatcgcctc gcgcgtcttc tcggccacac cgccgagcgt
      781 gcccgacgcc gggctcgggc ggatgtaggc agcttcatcg accgacagcc gttccatgcg
      841 cgtcttgtcg ccgaggatcg aaccaccgga caggctggac gacgggtcga tcgcgagcac
      901 cgccacgcga tgccccttgg cgatcagtgt caggcccagc gtctcgatga aggtcgactt
      961 gccgacgccg ggcacgcccg agatgcccag ccgcatgccg tggccggtgt gcggcagcaa
     1021 ggagttcagc agttcgtcgg cgcgcgcgcg gtgatcggcg cgcgtcgatt cgagcaaggt
     1081 gatggacttg gcgagcgcgc ggcgatcgcc cgcgcgcacg ccgctcacca gagcctcgtc
     1141 gacggaattc aacgccacgg attcagccct tggcgacgct catgcggatc tgttcgagca
     1201 cgtccttcgc gctcgccggg atcggcgtgc ccggcccgta gatgcccttc acgccggcat
     1261 cgaagagcat ctcgtagtcc tgccgcggga tcacgccgcc gacgaacacg atgatgtcgt
     1321 cggcgccctg ctgcctcagc gactcgatga tcgccggcac cagcgtcttg tggccggccg
     1381 cgagcgtgct gacgccgacc gcgtgcacgt cgttctcgat cgcctggcgc gcgcactcgt
     1441 cggcggtctg gaacagcggg cccatgtcga cgtcgaaacc gaggtcggcg aacgcggtcg
     1501 ccaccacctt cgcgccgcgg tcgtgaccgt cctgcccgag cttggcgatc atcacgcgcg
     1561 ggcgccggcc ctggtcttcg gcgaactgcg cgatctcggt cttcagcttg tcccagcctt
     1621 cggcgctgtc gtaggccgcg gcatagacgc cggtgacctt ctgcgtgtcg gcacggtgcc
     1681 ggccccaggc cttctcgagc gcatcggaca cctcgccgac ggtggcgcgc aatcgcaccg
     1741 ccttgatcga caggtcgagc aggttgcctt cgccggtttc ggcgcagcgc gtcagcgcgt
     1801 cgagcgccgc ctgcgtcttt gcgccctcgc gtgcggcgcg caactgcctg agccgcgcga
     1861 cctgattgtc gcgcaccatc gcgttgtcga tgtcgcgggt ctcgatcgcg tcctcggtgc
     1921 ccaatctgta cttgttgacg ccgacgatca cgtccttgcc cgagtcgatg cgcgcctgct
     1981 tctcggccgc gctggcctcg atcttcagct tggcccagcc gctgtccacc gccttgacca
     2041 tgccgcccat ggcctcgact tcctcgacga tcttccacgc cgcatcggcc atgtcctgcg
     2101 tgagcttctc catcatgtag ctgccggccc aggggtcgac gacgctggtg atgtgggtct
     2161 cttcctggat gatcagctgc gtgttgcgcg cgatgcgcgc gctgaactcg gtcggcagcg
     2221 cgatcgcctc gtcgagcgcg ttggtgtgca gcgactgcgt gccgccgaac accgcggcca
     2281 tcgcctcgat cgtcgtgcgc acgacgttgt tgtacgggtc ctgctcggtc agcgaccagc
     2341 ccgaggtctg gcagtgcgtg cgcagcatca ggctcttcgg gttcttggcg tcgaagccct
     2401 tcatgatctt ccaccacagc agccgcgcgg cacgcatctt ggccacttcc agatagaagt
     2461 tcatgccgat ggcccagaag aacgacagtc gcccggcgaa ctcgtcgacg tccaggccct
     2521 tcgcgagcgc cgccttcacg tactcgcggc cgtcggccag cgtgaacgcg agctcgagcg
     2581 cctggttcgc gccggcctcc tgcatgt
//
LOCUS       DATMJB010000650         2872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_89220, whole genome shotgun sequence.
ACCESSION   DATMJB010000650 DATMJB010000000
VERSION     DATMJB010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89220"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="VNU48_13755"
     CDS             complement(<1..659)
                     /locus_tag="VNU48_13755"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWI12393.1"
                     /translation="MPQTEPALAPRTTMRVIVTRPAAQAADWVAWLRAQGIDALALPL
                     IAIAPIADATPLVQAWNRLAAYRLVVFVSPNAAERFFAQRPAAQGWPPGVLAGSPGPG
                     TTRALLALGVPSAQLVEPAQDAPQFDSETLWAQMRGQDWHAAQVLIVRGDGGREWLAQ
                     TLRAAGARVAQVAAYRRTAPTFDAAQAGLLQAALAEPARHLWLFSSSEAIEHLLASPL
                     VG"
     gene            complement(646..1596)
                     /gene="hemC"
                     /locus_tag="VNU48_13760"
     CDS             complement(646..1596)
                     /gene="hemC"
                     /locus_tag="VNU48_13760"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWI12394.1"
                     /translation="MPETTLIATRESRLALWQAEHVRDLLTQRFGMAVGLLGMTTRGD
                     QILDRTLSKVGGKGLFVKELETALEEGRAQLAVHSLKDVPMDLPDGFELAAVLEREDP
                     RDAFVSPRYASLAELPPGATVGTSSLRRVVQLLAARPDLDVQPLRGNLDTRLRKLDDG
                     GFDAIVLAAAGLMRLGLAGRIASRFEPAAMLPCAGQGALGIEVRADASALRERLAQLT
                     HRPTWLAVLAERAVSRALGGSCSMPLAAHATWQGDTLALQVALGHTQQLRTPLLRTQA
                     GAAVQTDAQARALGERAAQQLRDAGAAGYLAAAQAADAAD"
     gene            1674..>2872
                     /locus_tag="VNU48_13765"
     CDS             1674..>2872
                     /locus_tag="VNU48_13765"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HWI12395.1"
                     /translation="MPRATTRLNDTRGAAAKNRPLVDDIRLLGRILGDVIREQEGKDA
                     FELVERVRRLSVAYRLKSDAQAGRALDRLLKNLSGDQTVSVIRAFSYFSHLANIAEDR
                     HHVRRRDHHEREGHLQEGSLAMTLERLARADIRAADIARTLAHAYISPVLTAHPTEVQ
                     RKSILDAERAIAELVEHRDTSANERERADNEALLRARVTQIWQTRMLRTAKLTVADEI
                     ENALSYYRSTFLRQIPKMYRELESALPGHVIAPFFRMGNWIGGDRDGNPFVTAATLQM
                     ALARQAETVLRFYLTEAHELGAELSISATLAPVTAEMAALAARSPDQNVHRADEPYRR
                     ALTGMYARLAATLELFTGTEALRHAVAPQNPYASSDEFLADLQVIDASLAAHHAQALS
                     RPRLQPL"
BASE COUNT          422 a         1102 c         1000 g          348 t
ORIGIN      
        1 ccgacgagcg gcgacgcgag cagatgttcg atcgcctcgg agctgctgaa cagccacagg
       61 tgccgcgcgg gttcggccag cgcggcctgc agcagcccgg cctgggccgc gtcgaaggtt
      121 ggcgccgtgc gccggtaggc ggcgacctgc gcgacccgcg cgccggccgc gcgcaaggtc
      181 tgcgcgagcc actcgcggcc gccgtcgccg cgcacgatca acacctgggc agcgtgccag
      241 tcctgcccgc gcatttgcgc ccagagcgtt tcggaatcga actgcggcgc gtcctgcgcc
      301 ggctcgacga gctgcgccga aggcaccccg agcgcgagca acgcgcgcgt cgtgcccggg
      361 cccggcgagc cggccagcac gcccggcggc cagccctgcg cggcggggcg ttgcgcgaag
      421 aaccgctcgg cggcgttggg gctcacgaac acgaccagcc gataggcggc gagccggttc
      481 cacgcctgaa ccagcggcgt ggcgtcggcg atcggcgcga tcgcgatcaa gggcagcgcg
      541 agcgcatcga tcccctgcgc gcgcaaccac gccacccaat cggcagcctg cgccgccggc
      601 cgcgtgacga tcacccgcat ggtcgtgcgc ggtgcgagcg ccggttcagt ctgcggcatc
      661 ggccgcctgc gccgcggcca ggtagccggc ggcgcccgcg tcgcgcaact gctgtgcggc
      721 gcgctcgccg agcgcgcggg cctgcgcatc ggtctgcacc gcggcgcccg cctgcgtgcg
      781 cagcaacggc gtgcgcaact gctgcgtgtg accgagcgcc acctgcagcg ccagcgtgtc
      841 gccctgccag gtcgcgtgcg cggccagcgg catgctgcag ctgccgccca aggcgcgcga
      901 cacggcgcgt tcggccagca ccgcgagcca ggtcgggcgg tgtgtgagct gcgcgagccg
      961 ttcgcgcagt gccgacgcgt cggcccgcac ctcgatgccg agcgcgccct ggccggcaca
     1021 cggcagcatc gcggcgggct cgaagcggct cgcgatgcgc cccgccagac cgagccgcat
     1081 cagccccgca gccgcgagca cgatcgcatc gaagccgccg tcgtcgagct tgcgcagccg
     1141 cgtgtcgagg ttgccgcgca gcggctgaac atccagatcg gggcgcgccg cgagcagctg
     1201 cacgacgcgg cgcaggctcg acgtgccgac ggtcgcgccg ggcggcagct cggccagcga
     1261 cgcgtagcgc ggcgacacga acgcatcgcg cggatcctcg cgctccagca ccgcggcgag
     1321 ctcgaagccg tcgggcaggt ccatcggcac gtccttcagc gagtgcaccg cgagctgcgc
     1381 gcgcccctcc tcgagcgcgg tctcgagctc cttgacgaac aggcccttgc cgccgacctt
     1441 ggacagcgtg cggtcgagga tctgatcgcc gcgcgtggtc atgccgagca ggccgacggc
     1501 catgccgaag cgttgcgtca gcaggtcgcg cacgtgctcg gcctgccaca gcgcgaggcg
     1561 gctttcgcgg gtggcgatca gggtggtctc gggcatgcgc gggatgctag caggcccgtc
     1621 tcttgcatcg cctcttaaga tcgacaggcc gccgcccatc caccgagagc cccatgcccc
     1681 gagccacgac tcgcctgaac gacacgcgcg gcgctgccgc gaagaaccgc ccgctggtcg
     1741 acgacatccg cttgctcggc cgcatcctcg gcgacgtgat ccgcgagcag gaaggcaagg
     1801 acgcgttcga gctggtcgag cgcgtgcgcc ggttgtcggt cgcctaccgc ctgaagagcg
     1861 acgcgcaggc cggccgcgcg ctcgaccggc tgctgaagaa cctgtcgggc gaccagaccg
     1921 tcagcgtgat ccgcgcattc agctacttct cgcacctggc caacatcgcc gaggaccgcc
     1981 accacgtgcg ccgccgcgac caccacgaac gcgaaggcca cctgcaggaa ggctcgctgg
     2041 cgatgacgct ggaacgcctg gcacgcgccg acatccgcgc cgccgacatc gcccgcacgc
     2101 tggcccacgc ctacatctcg ccggtgctga ccgcgcaccc gaccgaggtg cagcgcaaga
     2161 gcatcctcga cgccgagcgc gcgatcgccg aactggtcga gcatcgcgac acctcggcca
     2221 acgagcgcga gcgcgccgac aacgaggcgc tgctgcgcgc gcgcgtcacg cagatctggc
     2281 agacgcgcat gctgcgcacc gcgaagctga cggtggccga cgagatcgag aacgcgctga
     2341 gctactaccg ctcgaccttt ctgcgccaga tcccgaagat gtaccgcgag ctcgagagcg
     2401 ccctgcccgg ccacgtcatc gcgccgttct tccgcatggg caactggatc ggcggcgacc
     2461 gcgacggcaa ccccttcgtc accgccgcga ccctgcagat ggcgctggcg cgccaggccg
     2521 agacggtgct gcgcttctac ctgaccgagg cgcacgaact cggcgccgag ctgtcgatct
     2581 cggccacgct ggctccggtg acggccgaga tggccgcgct ggccgcgcgc tcgcccgacc
     2641 agaacgtgca ccgcgccgac gagccctacc gccgcgcgct gaccggcatg tacgcgcggc
     2701 tggccgcgac gctcgaactc ttcaccggca ccgaggcgct gcgccatgcg gtcgcgccgc
     2761 agaacccgta tgcctcgtcg gacgagttcc tcgccgacct gcaggtcatc gatgcctcgc
     2821 tcgccgcgca ccacgcgcag gcgctcagcc gcccgcggct gcagccgctg at
//
LOCUS       DATMJB010000651         2850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_808741, whole genome shotgun sequence.
ACCESSION   DATMJB010000651 DATMJB010000000
VERSION     DATMJB010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808741"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VNU48_13770"
     CDS             <1..271
                     /locus_tag="VNU48_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWI12396.1"
                     /translation="WKDRYDKKYPGQFQVYSPYTYDATHVLVQAMIDAGSADPKVYLP
                     VLAKTDGYKGVTGTISFDEKGDIKNGALTLHSYKGGKETSLGVVH"
     gene            complement(319..2427)
                     /gene="glgX"
                     /locus_tag="VNU48_13775"
     CDS             complement(319..2427)
                     /gene="glgX"
                     /locus_tag="VNU48_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HWI12397.1"
                     /translation="MESLKPGAASFAIGPGRAWPIGAHADATGVNFAVFSAHAQAIEL
                     CLFDVTGTHEVARLRLPGRSGDVWHGRVEGAAPGLVYGLRALGAWRPDKGHRFNPAKL
                     LLDPYAREIVGRFVWSDEHFAADHTHPKQMDTRDNAATALKARVTHDAHDWGDDRAPL
                     TPLADTVLYEMHVKGFSKLNQRVPEALRGTYAGLAHEASIAHLKRLGVTAVSLLPVHQ
                     HIDEERLVAMGLVNYWGYNTIAFFAPDPCLSSTPDDAAATRREFREMVSSLHAAGIEV
                     ILDVVFNHTAESDATGPTISLRGLDNATYYRLPPDARHEYINDTGCGNTVDLRHPRVL
                     QLVMDSLRYWVTEMHVDGYRFDLATVLGRGDEGFDRRAAFFAAIAQDPVLARVKMIAE
                     PWDIGPGGYQVGNFPNGWAEWNDKFRDDVRAFWLRHPSTRGAFAQRLCASSDLYQARG
                     RAPAESLNYVISHDGFTLRDLVSYNERHNEANGEHNRDGTAHNISFNAGAEGPSNDPA
                     VTLLRARLARAVLATNLLAQGTPMISAGDELGHSQGGNNNPYCQDNATTWIAWSAADE
                     DMIAFAIRVLALRRIARPYGTHWYSGLTDPLGLHDLAWLSRAGEPLRAADWSDPNDRV
                     LGCLIGDPGRAAAPLLLLVNASADDSNFMLPAGVWEVVLDSTHPRGVGSWHGQGEVAL
                     PLSAASLMLLAAAGADIKLA"
     gene            complement(2415..>2850)
                     /locus_tag="VNU48_13780"
     CDS             complement(2415..>2850)
                     /locus_tag="VNU48_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HWI12398.1"
                     /translation="LDWHLLDDPMHDGVRRLLRDLNALYRATPALYQRDFSPDGFEWI
                     DHADAEHSVLAFIRRGHSANALVVVACNFTPTPQRAYRLGVPHGGTYRECINTDSMHY
                     GGSNVGTPLGAATAQDLPQHGRPHSIVIDLPPLATVMLEWKA"
BASE COUNT          409 a          942 c         1049 g          450 t
ORIGIN      
        1 ctggaaggac cgctacgaca agaagtaccc gggccagttc caggtctact cgccgtacac
       61 ctacgacgcg acgcatgtgc tggtgcaagc gatgatcgac gccggctcgg ccgatccgaa
      121 ggtctacctg ccggtgctgg ccaagaccga tgggtacaag ggtgtgaccg gcacgatctc
      181 gttcgacgag aagggcgaca tcaagaacgg cgcgctgacc ctgcacagct acaagggcgg
      241 caaggaaacg tcgctcggcg tggttcactg aaccgcgggc ttcgcgacga ttcaaggggc
      301 gccctcgggc gccctttcct atgccagctt gatgtcggcg ccggccgccg ccagcagcat
      361 caggctggcc gcggacaagg gcagtgcgac ttccccttgc ccatgccacg agcccacgcc
      421 gcgcgggtgc gtgctgtcga gcacgacctc ccagacgccg gccggcagca tgaagttgct
      481 gtcgtccgcg ctggcgttga ccagcagcag cagcggcgcc gcggcacggc ccgggtcgcc
      541 gatcaggcag ccgagcacac ggtcgttcgg atcgctccag tcggcggctc gcagcggctc
      601 accggcgcgc gagagccacg ccaggtcgtg caggccgagc gggtcggtca ggccgctgta
      661 ccagtgcgtg ccatagggcc gcgcgatgcg gcgcagcgcc agcaccctga tggcgaacgc
      721 gatcatgtcc tcgtcggccg ctgaccaggc gatccaggtg gtcgcgttgt cctgacagta
      781 ggggttgttg ttgccgccct ggctgtggcc gagctcgtcg ccggcgctga tcatcggcgt
      841 gccttgcgcg agcaggttgg tcgcgagcac ggcgcgggcg aggcgcgcgc gcagcagcgt
      901 gacggccggg tcgttgctcg gcccttcggc acctgcattg aagctgatgt tgtgggcggt
      961 gccgtcgcga ttgtgctcgc cgttggcctc gttgtggcgc tcgttgtagc tgaccaggtc
     1021 gcgcagcgtg aaaccgtcgt gagagatcac gtaattgagc gactcggccg gcgcacgccc
     1081 gcgcgcctgg tagaggtcgc tcgaggcgca cagccgctgc gcgaacgcgc cgcgcgtgct
     1141 cgggtggcgc agccagaacg cgcgcacgtc gtcgcggaac ttgtcgttcc actcggccca
     1201 gccgttcgga aagttgccga cctggtagcc gccggggccg atgtcccagg gctccgcgat
     1261 catcttgacg cgcgcgagca ccgggtcctg cgcgatcgcg gcgaagaacg cggcgcggcg
     1321 gtcgaagccc tcgtcgccgc ggccgagcac cgtggccagg tcgaagcggt aaccgtcgac
     1381 gtgcatctcg gtcacccagt agcgcaggct gtccatcacg agttgcagca cgcgcgggtg
     1441 gcgcaggtcg actgtgttgc cgcagccggt gtcgttgatg tactcgtggc gcgcgtcggg
     1501 cggcagccgg taataagtgg cgttgtcgag cccgcgcaag ctgatggtcg ggccggtggc
     1561 gtcgctctcg gcggtgtggt tgaagaccac gtcgagaatg acttcgatgc ccgccgcatg
     1621 cagcgagctc accatctcgc ggaactcgcg gcgggtcgcg gcggcgtcgt ccggcgtgct
     1681 gctcaggcag gggtcgggcg cgaagaaggc gatcgtgttg tagccccagt agttgaccag
     1741 gcccatcgcg acgaggcgct cctcgtcgat gtgctggtgc acgggcagca ggctcacggc
     1801 ggtcacgccc aggcgcttca ggtgtgcgat cgaggcctcg tgggcgagcc ccgcgtaggt
     1861 gccgcgcagc gcttccggca cgcgctggtt gagcttcgag aaacccttga cgtgcatctc
     1921 gtacagcacc gtgtcggcca gcggcgtcag cggcgcgcga tcgtcgcccc agtcgtgcgc
     1981 gtcgtgggtg acgcgggcct tcagcgcggt ggccgcgttg tcgcgcgtgt ccatctgctt
     2041 cgggtgcgtg tggtcggcgg cgaagtgctc gtcgctccac acgaagcgcc cgacgatctc
     2101 gcgcgcgtag gggtcgagca gcagcttggc cgggttgaag cggtggccct tgtcgggccg
     2161 ccacgcgccg agcgcgcgca ggccgtagac caggccgggc gcggcgcctt cgacacggcc
     2221 gtgccagaca tcgccgctgc gccccggcag gcgcaggcgc gcgacctcgt gtgtgccggt
     2281 cacgtcgaac aggcacagct cgatcgcctg cgcatgcgcc gagaacaccg cgaagttgac
     2341 acccgtggcg tcggcgtgcg cgccgatcgg ccacgcgcgc ccgggtccga tcgcgaacga
     2401 cgcggcgccc ggcttcaggc tttccattcc agcatcacgg tcgccagtgg cggcaggtcg
     2461 atcacgatcg agtgcggccg gccgtgctgc ggcaggtcct gtgccgtcgc agcgcccagc
     2521 ggcgtgccga cgttgctgcc gccgtagtgc atcgagtcgg tgttgatgca ttcgcggtag
     2581 gtgccgccgt gaggcacgcc caggcggtac gcgcgctgcg gcgtgggcgt gaagttgcag
     2641 gccacgacga ccagcgcatt cgccgaatgg ccgcgacgga tgaaggcgag caccgagtgc
     2701 tcggcgtcgg catggtctat ccactcgaag ccgtcgggcg agaagtcgcg ctggtacagc
     2761 gccggcgtgg cgcggtagag cgcgttcagg tcgcgcagca gcctgcgcac gccgtcgtgc
     2821 atcgggtcgt cgagcaggtg ccagtccagg
//
LOCUS       DATMJB010000652         4974 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_801014, whole genome shotgun sequence.
ACCESSION   DATMJB010000652 DATMJB010000000
VERSION     DATMJB010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801014"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VNU48_13785"
     CDS             complement(<1..411)
                     /locus_tag="VNU48_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI12399.1"
                     /translation="MNWPYELQIGWRYTRAGRSGRRNGFISFISGVSMLGIGLGVAAL
                     IIVLSVMNGFRKEVQGQMLAAVPHIEVATADGAALPDWKATLAQIEAVPQAGAQVIGA
                     APFISTQALLARGDEMRGVILRGIAPNDEAGVTDV"
     gene            490..1431
                     /locus_tag="VNU48_13790"
     CDS             490..1431
                     /locus_tag="VNU48_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12400.1"
                     /translation="MQLLIPFASGLSDAASPVLRDLALPNLTQLLTLLTPTQRDESDA
                     YTLSPPHERALAAAAGWHGADGCLPFAAQAAARDGLEVGERAWGLVTPAHWHVGRDHV
                     TLVDPAALELSDDESRAAFEVVRALFETEGFAFAWGAASRWYAAHDSLAELPCASLDR
                     VIGRNVELWMRGSAARNAAAQRIRRLQSEVQLLLYPHALNEEREQRGALPLNSFWLSG
                     CGRRQPDATTPIIVNDTLRAPLLADDWAAWAEAWRALDAGDLAELLAAVRAGRAGTLT
                     LCGERSAQRFENLPRALGQRLRARWQAAAPHTVLEAL"
     gene            1431..3200
                     /gene="recJ"
                     /locus_tag="VNU48_13795"
     CDS             1431..3200
                     /gene="recJ"
                     /locus_tag="VNU48_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HWI12401.1"
                     /translation="MAAHATPTIRLRDAPPRAVWALEQAGVPPLLARLFAGRGVRTAD
                     ELDEGLARLLPPGDLLGVQAAARFLADTIGQQRRVCVVADYDCDGATACAVALRGLRL
                     LGAAADTLHYVVPDRAVHGYGLTPAIVDLARARVPKGPDVLLTVDNGIASLQGVAHAR
                     ALGIAVLVTDHHLPARIDDLVVLPDANVIVNPNQPGCGFASKNLAGVGVIFYVLLALR
                     AELRERGVFTAQDQPRLDALLDLVALGTVADVVRLDANNRRLVAQGLRRIRAGQMQPG
                     VAALFSAAGRDARRASGFDFGFALGPRINAAGRLSDMTLGIECLLTDDAARAAELAQL
                     LDTINRERREVESGMREQAEAMLARLLQSDDAAASRTPPALAIFDEGFHEGVVGIVAS
                     RLKDRVHRPAFVFARGQDGLLKGSGRSIPGFHLRDALDLLSKRHPEVLLRFGGHAMAA
                     GCTLAEAQLATFDAAFQRIAAEWLDAATLSRTLLADGPLGIEHFNAATVRALDAQVWG
                     QAFEPPVFVDAVEIVAQRLVGEKHLKLSLRYVGEARGAPLRDAIWFGHSEPVAATLRL
                     AYRLSVDEYNGRERVQMIVEGIG"
     gene            3284..4402
                     /locus_tag="VNU48_13800"
     CDS             3284..4402
                     /locus_tag="VNU48_13800"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWI12402.1"
                     /translation="MRPLRPALAAALAAAVLVLAHGLAAAQALKAITVARGLVNPWGL
                     AFLPDGRMLVTERPGRLRVVTPDGRLGEPLQGLPAVEVGGQGGLLDVAIDPRFAANGL
                     VYWSFSETDPQGGRGNSTAVARGRLDGNRLVDVRVIFRQAPKFASSAHFGSRLVFAPD
                     GRLFVTLGDRFLRRDDAQSLANHHGKIVRIESDGRVPADNPFVGRAGALPEIWSYGHR
                     NVQGAAINPATGELWVDEHGPQGGDELNVVLAGRNYGWPVITYGAEYVTGAKIGAGAS
                     QSGLEQPLTYWVPSIATSGLAFLTSDRYPGWKGSAFIGALKAQLLVRLELGGRRAVRE
                     ERLLQDLGERIRDVRQGPDGWLYVLTDSANGRILRLER"
     gene            4455..>4974
                     /locus_tag="VNU48_13805"
     CDS             4455..>4974
                     /locus_tag="VNU48_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWI12403.1"
                     /translation="MTTPNALNADLHCHSAVSDGTLEPEALAARAKANGVQLWALTDH
                     DEIGGQQRARAAAAALGLPYLTGTEISVTFADTTVHIVGLGFDADDAALVAGLHATRG
                     GRRARAMEMSDGLAKVGIRGAYDGALQYVGNPELISRTHFARFLVETRVCADTQEVFR
                     KYLVEGKPGFVPH"
BASE COUNT          657 a         1835 c         1812 g          670 t
ORIGIN      
        1 gacgtcggtc acgccggcct cgtcgttcgg cgcgatgccg cgcaggatca cgccgcgcat
       61 ctcgtcgccg cgcgcgagca gcgcctgggt cgagatgaac ggcgcggcgc cgatcacctg
      121 cgcgccggcc tgcggcacgg cctcgatctg cgcgagcgtc gccttccagt cgggcagcgc
      181 agcgccatcg gccgtcgcca cctcgatgtg cggcaccgcc gcgagcatct ggccctgcac
      241 ttccttgcga aagccgttca tcaccgagag cacgatgatc agcgccgcga cgccgagccc
      301 gatgcccagc atcgacacgc cggagatgaa cgagatgaag ccgttgcgcc gcccactgcg
      361 gccggcgcgg gtgtaacgcc agccgatctg caactcgtag ggccagttca tggggaggcg
      421 atgctacagg acgcatcgcg cgcctcgcgg ccgcggggca caagtggcag cgcccggata
      481 atccgcgcca tgcagctgct gatccccttc gcgtcgggcc tgtccgacgc cgcgtcgccg
      541 gtgctgcgcg atctggcatt gcccaacctg acgcagctgc tgacgctgct cacgccgacg
      601 caacgcgacg agtccgacgc ctacaccttg tcgccgccgc acgaacgcgc gctcgcggcc
      661 gcggccggct ggcacggcgc cgacggctgc ctgccgttcg ccgcgcaggc cgccgcgcgc
      721 gacggcctcg aggtcggtga gcgcgcctgg ggcctggtca cgccggcgca ctggcacgtc
      781 ggccgcgacc acgtcacgct ggtcgacccg gccgcgctcg agctgagcga cgacgaatcg
      841 cgcgccgcgt tcgaggtggt gcgcgcgctg ttcgagaccg agggctttgc cttcgcgtgg
      901 ggcgcggcct cgcgctggta cgccgcgcac gacagcctcg ccgagctgcc gtgcgcgtcg
      961 ctggaccgcg tgatcggccg caacgtcgaa ctctggatgc gcggcagcgc ggcgcgcaac
     1021 gcggcagccc aacgcatccg ccgactgcag agcgaagtgc agctgctgct ctacccgcac
     1081 gcgctgaacg aggaacgcga gcaacgcggc gcgctgccgc tgaactcgtt ctggctcagc
     1141 ggttgcggcc ggcgccagcc cgatgcgacc acaccgatca tcgtcaacga cacgcttcgc
     1201 gcgcccttgc tcgccgacga ctgggccgcg tgggccgagg cctggcgcgc gctcgatgcg
     1261 ggcgatctgg ccgagctgct cgcggcggtc cgtgccggtc gcgcaggcac gctgacgctg
     1321 tgcggcgagc gcagcgcaca gcgcttcgag aacctgccgc gggcgctggg ccaacggctg
     1381 cgcgcgcgct ggcaggccgc cgcgccgcac accgtgctgg aggcactgtg atggccgccc
     1441 acgcgacgcc gacgatccgg ctgcgcgacg cgccgccgcg tgccgtctgg gcgctcgaac
     1501 aggccggcgt gccgccgctg ctggcgcggc tgttcgccgg ccgcggcgtg cgcacggcag
     1561 acgagctcga cgagggcctg gcgcgcctgc tgccgcccgg cgatctgctc ggcgtgcagg
     1621 ccgccgcgcg cttccttgcc gacacgatcg ggcagcagcg gcgcgtctgc gtcgtcgccg
     1681 actacgactg cgatggcgcg accgcctgcg cggtcgcgct gcgcggcctg cgtctgctgg
     1741 gcgccgcagc ggacacgctg cactatgtcg tgccggaccg cgcggtgcac ggctacggcc
     1801 tcacgccggc gatcgtcgac ctggcgcgcg ccagagtgcc gaaggggccc gacgtgctgc
     1861 tcacggtcga caacggcatc gccagcctgc agggcgtggc gcacgcacgc gcgctgggca
     1921 tcgcggtgct ggtgaccgac caccacctgc cggcgcgcat cgacgatctc gtggtgctgc
     1981 ccgacgcgaa cgtgatcgtg aacccgaacc agccgggctg cggcttcgcg agcaagaacc
     2041 tggccggcgt cggcgtgatc ttctacgtgc tgctcgcgct gcgcgccgag ctgcgcgagc
     2101 gcggcgtgtt cacggcgcag gatcagccgc ggctcgatgc gctgctcgat ctggtcgcgc
     2161 tcggcaccgt ggccgacgtg gtccggctcg atgccaacaa ccggcgcctg gtggcgcagg
     2221 gcctgcgccg catccgcgcc gggcagatgc agccgggcgt ggcggcgctg ttcagcgccg
     2281 cggggcgcga tgcgcgccgc gccagcggct tcgacttcgg cttcgcgctc gggccgcgca
     2341 tcaacgccgc gggccggctg tcggacatga ccctgggcat cgaatgcctg ctgaccgacg
     2401 acgcggcgcg cgccgccgag ctcgcgcagc tgctcgacac gatcaatcgc gaacgccgcg
     2461 aggtcgagtc cggcatgcgc gagcaggccg aggcgatgct agcgcggctg ctgcagagcg
     2521 acgacgcggc cgcgagccgc acgccgccgg cgctggcgat cttcgacgaa ggctttcacg
     2581 aaggcgtggt cggcatcgtc gcgtcgcgcc tgaaggaccg cgtgcaccgc ccggccttcg
     2641 tgttcgcgcg cggccaggac gggctgctga agggctcggg ccgctcgatc ccgggcttcc
     2701 atctgcgcga cgcgctcgac ctgctcagca agcgccaccc cgaggtgctg ctgcgcttcg
     2761 gcggccatgc gatggcggcc ggctgcacgc tggccgaggc gcagctcgcg accttcgatg
     2821 ccgcgttcca gcgcatcgcg gccgagtggc tggacgcggc caccttgtcg cgcacgctgc
     2881 tcgccgacgg cccgctgggc atcgagcact tcaacgcggc gacggtgcgc gcgctcgacg
     2941 cgcaggtctg gggccaggcc ttcgagccgc cggtgttcgt cgacgcggtc gagatcgtcg
     3001 cgcagcgcct ggtcggcgag aagcacctga aactgtcgtt gcgctatgtc ggcgaggcgc
     3061 gcggcgcacc gctgcgcgat gcgatctggt tcgggcacag cgagccggtg gcggcgacgc
     3121 tgcggctggc ttaccgtttg agcgtcgacg agtacaacgg ccgcgaacgg gtgcagatga
     3181 tcgtcgaagg tatcggctga cggcctgcac gcccgggctt gcacggcggc cgatgatggc
     3241 ccggcgcggc gcgcgggttt gggcgcacgg gcatgctgcg ccgatgagac cgctccgccc
     3301 cgccctcgcc gccgcgctcg cggctgcggt gctggtgctt gcgcacggcc tggccgcggc
     3361 gcaggcgctg aaggcaatca cggtggcccg tgggctcgtc aacccctggg gcctcgcgtt
     3421 cctgcccgac ggccgcatgc tcgtgaccga gcggccgggg cgcctgcgcg tggtcacgcc
     3481 cgacggtcgg ctcggcgagc cgctgcaggg cctgccggcg gtcgaggtcg gcggccaggg
     3541 cgggctgctc gatgtcgcga tcgacccgcg cttcgcggcc aacggcctgg tctactggag
     3601 cttcagcgag accgatccgc agggcgggcg cggcaacagc accgcggtgg cgcgcggccg
     3661 gctcgacggc aaccggctgg tcgacgtgcg cgtgatcttc cgccaggcgc cgaagttcgc
     3721 gagcagcgcg cacttcggtt cgcggctcgt gttcgcgccc gatggccggc tcttcgtcac
     3781 gctcggcgac cgcttcctgc ggcgcgacga cgcgcagtcg ctggccaacc accacggcaa
     3841 gatcgtgcgc atcgagtccg acggccgggt gccggccgac aacccgttcg tcggccgcgc
     3901 cggcgcgctg cccgagatct ggagctacgg ccaccgcaac gtgcagggcg cggcgatcaa
     3961 ccccgccacc ggcgagctgt gggtcgacga acatggcccg caaggcggcg acgaactcaa
     4021 cgtcgtgctc gccgggcgca actacggctg gccggtgatc acctacggcg ccgagtacgt
     4081 caccggcgcg aagatcggcg caggcgcctc gcagtcgggc ctggaacagc cgctgaccta
     4141 ctgggtgccg tcgatcgcca ccagcggcct ggccttcctg accagcgacc gctacccggg
     4201 ctggaagggc agcgcgttca tcggcgcgtt gaaggcgcag ctgctggtgc ggctcgagct
     4261 cggcggccgc agggccgtgc gcgaggaacg gctgctgcag gacctgggcg aacgcatccg
     4321 cgacgtgcgc cagggcccgg acggctggct ctacgtcctc accgacagcg cgaacggccg
     4381 catcctgcgg ctcgaacgct gagcccggcg ggatcgcgcc cggcacgccg cacccacgct
     4441 cctacaatgc gatcgtgacc acaccgaacg cgctgaacgc cgacctgcac tgccattccg
     4501 ccgtgtccga cggcacgctg gaacccgagg ccttggccgc gcgggcgaag gccaatggcg
     4561 tgcagctgtg ggcgctgacc gatcacgacg agatcggcgg ccagcagcgc gcacgcgccg
     4621 ccgccgcggc gctcggcctg ccttacctga ccggcaccga gatctcggtg accttcgccg
     4681 acacgaccgt gcacatcgtc ggcctcggct tcgacgccga cgatgcggcg ctggtcgccg
     4741 ggctgcacgc cacgcgcggc gggcgccgcg cgcgcgcgat ggaaatgtcg gatgggctgg
     4801 ccaaggtcgg catccgcggc gcctacgacg gtgcgctgca atacgtcggc aaccccgagc
     4861 tgatctcgcg cacccacttc gcgcgcttcc tggtcgagac ccgggtctgc gccgacacgc
     4921 aagaggtgtt ccgaaagtac ctggtcgaag gcaagccggg cttcgtgccg cacc
//
LOCUS       DATMJB010000653         2020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_801116, whole genome shotgun sequence.
ACCESSION   DATMJB010000653 DATMJB010000000
VERSION     DATMJB010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801116"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..901
                     /gene="hisH"
                     /locus_tag="VNU48_13810"
     CDS             254..901
                     /gene="hisH"
                     /locus_tag="VNU48_13810"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWI12404.1"
                     /translation="MRRTVAVVDYGMGNLRSVSQAVLHVARDADVDVIVTSRPDEVFA
                     AERVVLPGQGAMPDCMRELRESGLQEAVLHAAANKPLMGVCVGMQMLLSRSDEGPTDG
                     LGLIAGEVVRFRLEGQLQPDGSRYKVPQMGWNRVYQARPHALWQGVPDGAYFYFVHSF
                     YARPSAPGHSVGETDYGTRFTSALARDNIFATQFHPEKSAEHGLALYRNFLHWTP"
     gene            925..1668
                     /gene="hisA"
                     /locus_tag="VNU48_13815"
     CDS             925..1668
                     /gene="hisA"
                     /locus_tag="VNU48_13815"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HWI12405.1"
                     /translation="MLLIPAIDLKDGHCVRLKQGDMNDSTTFSEDPARMARRWVDAGA
                     RRLHLVDLNGAFAGKPVNEGAIKAILAEVGEEIPVQLGGGIRDLDTIERYLDDGLSYI
                     IIGTAAVKNPGFLRDACSAFGGHIVVGLDAKEGKVATDGWSKLTGHEVIDLAKKFQDY
                     GVEGVIYTDIGRDGMLSGINIDATVKLAQALTIPVIASGGLSGMADIDQLCAVQDQGV
                     QGVICGRAIYSGDLDFAAAQARADELNGA"
     gene            1673..>2020
                     /locus_tag="VNU48_13820"
     CDS             1673..>2020
                     /locus_tag="VNU48_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HWI12406.1"
                     /translation="MLAKRIIPCLDVTGGRVVKGVNFVELRDAGDPVEIAARYNDQGA
                     DELTFLDITATSDGRDLILHIIEAVASTVFIPLTVGGGVRKVEDVRRLLNAGADKVSM
                     NTSAVTNPQLVFEA"
BASE COUNT          333 a          698 c          688 g          301 t
ORIGIN      
        1 cagcgaacgc agtgagcgca gtgagttctg cggcgcgacc ccggaccgag cagcgcagtg
       61 cagtcggtgc gcagcaccga ccgccaccgt atgagcccgc gccgggcagc gcctgccgcg
      121 acgcgccgac gtctgcaagc agtggtcgtc cgcgaacgac cgcatcgggc cgcaagccgg
      181 cgctcggtga cgaacagccg cgtttcagtt tgcggttccc gcggaccgcc cgcagggcgg
      241 cggagccact ggcgtgagac gcacggtcgc cgtcgtcgac tacggcatgg gcaacctgcg
      301 ctcggtgtcg caggcggtgc tgcatgtggc gcgcgatgcg gacgtggacg tgatcgtcac
      361 ctcgcgaccc gacgaggtgt tcgctgccga gcgcgtcgtg ctgccgggcc agggcgcgat
      421 gcccgactgc atgcgcgagc tgcgcgagtc cggcctgcaa gaggccgtgc tgcacgcggc
      481 ggccaacaag ccgctgatgg gcgtgtgcgt gggcatgcag atgctgctgt cgcgcagcga
      541 cgaagggcca accgacgggc tgggcctgat cgccggcgag gtcgtgcgct tccggctcga
      601 gggccagctg caacccgacg gcagccgcta caaggtgccg cagatgggct ggaaccgcgt
      661 ctaccaggcg cggccgcacg ccctgtggca gggcgtgccg gacggtgcgt acttctattt
      721 cgtgcacagc ttctacgccc gcccgtcggc gcccggccac agcgtcggcg aaaccgacta
      781 cggaacgcgc tttacctcgg cgctggcgcg cgataatatt ttcgccaccc agttccaccc
      841 cgaaaagagc gccgaacacg gcttggcgct ttaccgcaac ttcctccact ggaccccgtg
      901 aacgggtccc ttgccctgcc ggccatgttg ctcattcccg cgatcgacct gaaagacggt
      961 cactgtgtgc gcctcaagca gggcgacatg aacgactcca ccaccttcag cgaagacccg
     1021 gcgcgcatgg cgcggcgctg ggtcgatgcc ggcgcgcgcc gcctgcatct ggtcgacctg
     1081 aacggtgcgt tcgccggcaa gccggtcaac gagggggcga tcaaggcgat cctggccgag
     1141 gtcggcgagg agatcccggt gcagctcggc ggcggcatcc gcgatctgga caccatcgag
     1201 cgctacctcg acgacggcct ctcctacatc atcatcggca ccgcggcggt caagaacccg
     1261 ggcttcctgc gcgatgcctg ttcggccttc ggcggccaca tcgtcgtcgg gctggacgcg
     1321 aaggaaggca aggtcgccac cgacggctgg agcaagctca ccggccacga ggtgatcgat
     1381 ctggcgaaga agttccagga ctacggcgtc gaaggcgtga tctacaccga catcggccgc
     1441 gacggcatgc tctcgggcat caacatcgac gcgaccgtca agctcgcgca ggcgctgacg
     1501 atcccggtga tcgcctcggg cggcctgtcc ggcatggccg acatcgacca gctctgcgcg
     1561 gtgcaggacc agggcgtcca gggcgtgatc tgcggtcgcg cgatctacag cggcgatctc
     1621 gattttgccg ccgcgcaggc gcgcgccgac gaactcaacg gcgcctgaac gcttgctcgc
     1681 caaacgcatc atcccctgcc tcgacgtgac cggcgggcgc gtcgtcaagg gcgtcaactt
     1741 cgtcgagctg cgcgacgcgg gcgacccggt cgagatcgcg gcgcgctaca acgatcaggg
     1801 cgcagatgaa ctcacgttcc tcgacatcac cgccaccagc gacgggcgcg acctgatcct
     1861 gcacatcatc gaggcggtcg cctcgacggt gttcattccg cttaccgtgg gcggcggcgt
     1921 gcgcaaggtc gaggacgtgc gccggctgct gaatgccggc gccgacaagg tgtcgatgaa
     1981 tacctcggcc gtcaccaacc cgcagctggt attcgaggcc
//
LOCUS       DATMJB010000654         4754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_859723, whole genome shotgun sequence.
ACCESSION   DATMJB010000654 DATMJB010000000
VERSION     DATMJB010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859723"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1132)
                     /locus_tag="VNU48_13825"
     CDS             complement(128..1132)
                     /locus_tag="VNU48_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HWI12407.1"
                     /translation="MRLEERGAKSLRLSTCELAVARRLPALRDLFDRSIGMDIDAEPG
                     HPIDIQMNIAPGLRRARMLSPFTARAARPQTRLADGDDTVCLMIKTGGQMALSQGRHE
                     GVPQLGDAVLLVYRQPSRLEFVDATYLSVRVPFAALAMLADVEAAAACRIPRHSAALA
                     LLQNYVGSLPEHLDDPFLGRLAATHVYDLMALAIGATDEGREGARGRGVRAARLEAIK
                     TDLARDATLPIDQIARRQGVSTRYVQMLFEEQNTTFTEFAVDRRLDAALGMLTSPRYA
                     SWTIAGIALEAGFGDLSHFNRRFKRRFRATPTDVRQRTRDGPLFEDRDQEASSPPTGP
                     "
     gene            1326..1757
                     /locus_tag="VNU48_13830"
     CDS             1326..1757
                     /locus_tag="VNU48_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWI12408.1"
                     /translation="MVKPLWAASTLVAALVLANPLAAQQSAPASPSAAPNVKRIPLQR
                     FDVPGTAFEAVIGIAEIAPSVAIGRHTHPGPESGYVIEGGFELLIDGEPPRLLKAGDS
                     YKVPPGAIHDAKAGPQGAKVIATYVVEKGQPLAAAAPAAPQ"
     gene            complement(1902..2183)
                     /locus_tag="VNU48_13835"
     CDS             complement(1902..2183)
                     /locus_tag="VNU48_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12409.1"
                     /translation="MRSIPPRNHESAITNYFDATAKAIDPNRELVIGTPQRGACPMGG
                     SSGGYVGWVVPVEYKTRTKDSTTVTVTRYFFWFSDEVIRGVTRRMEVCP"
     gene            2603..3139
                     /locus_tag="VNU48_13840"
     CDS             2603..3139
                     /locus_tag="VNU48_13840"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HWI12410.1"
                     /translation="MRLMAVGVFVVAAAGATFSAHAQDRHGPGMGGGAHGDMMMFEGP
                     GGRGGRGLDRMLDGLNATAAQRAQIKQIAMAAATDLKAQREAERALREKGLQIFTAPT
                     VDAAAAESLRQQMSAQRDEASKRRLQAMLDIAKVLTPEQRAKIGERMKERQAVMKDRM
                     QRMQQERAERGQRPPAQK"
     gene            3241..3957
                     /locus_tag="VNU48_13845"
     CDS             3241..3957
                     /locus_tag="VNU48_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI12411.1"
                     /translation="MTARLLLIDDDARLSSMVGDYLRNSGFDVETAGSLAGGRERLAA
                     EHFDALVLDLMLPDGDGLDLCRELRSEGRHRHLPLLMLTARGEPMDRIVGLELGADDY
                     LPKPFEPRELLARVKALLRRAAPQPAGDDVLRFGRLEVDLGARVARLDGAICDLTGHQ
                     FDLLVVLAQSPGRVLSRDQIMDSLKGHPLEAFDRSIDVHISRIRAVIEDDPKLPRRVL
                     TVRGAGYVFARKQDADEARE"
     gene            3998..>4754
                     /locus_tag="VNU48_13850"
     CDS             3998..>4754
                     /locus_tag="VNU48_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HWI12412.1"
                     /translation="MKNLYLRIYATVVVVLLLFAAASGWIYQRHMEQERGRAEAVLTE
                     RMGAWAELIERSLPGHEMPPADQGAALRDWSQRLRLPLALDSADGVRIAESESFTRRQ
                     AEGAVRGLAVRLDDGRTLWIMRPGLMRSGGGRLGLGGAPPGVRPGGGEDRPPFLPFIP
                     AAWQGVGLVVVLAMLFVAVSAGAYPVVRRLTRRLEALKQGVEQFGAGQLHHRVQVTGH
                     DEVAAVASSFNVAAARVEALVRSHQSLLANASHE"
BASE COUNT          737 a         1632 c         1655 g          730 t
ORIGIN      
        1 cgccgccagg cgcgcgacaa aggtggcgat ccattgatcc tgcatgccgg tctcacgaac
       61 gaacacatct cctgcgcgac ctcaaagtgt cggtcggcgc agtgtgccgc gcaaatcgga
      121 cgaggcgcta cggccctgta ggaggtgaag atgcctcttg gtctcggtcc tcgaacagcg
      181 gcccgtcacg ggttcgttgg cgcacgtcgg tcggcgtcgc ccggaaccgc cgcttgaagc
      241 gccggttgaa gtgcgacaaa tcgccaaacc ctgcctcgag cgcaatgcca gcgatcgtcc
      301 aggaggcgta gcgggggctt gtcagcattc cgagcgccgc gtccaggcgc ctgtcgacgg
      361 cgaactcggt gaatgtggtg ttctgttcct caaacagcat ctggacatac cgcgtcgata
      421 caccttggcg ccgcgcaatc tggtcgatgg gcagtgtcgc gtcgcgcgcc aggtcggtct
      481 tgatcgcttc aagtcttgca gcacgcactc cgcggccgcg cgcgccctcc cggccttcgt
      541 cggtggcgcc aatggccagc gccatcaagt cgtaaacatg cgtggccgcg aggcgaccca
      601 ggaacggatc gtctaggtgc tcaggcaggc tcccgacata gttttgcagt agcgccagcg
      661 cggctgaatg gcgcggaatt cggcaggctg cggcggcttc gacgtcggcc agcatcgcca
      721 gcgccgcgaa cggcacacgt acagacaggt aggtggcatc cacgaattcc agtcgcgacg
      781 gttgtcgata gacgagcagt accgcgtcgc caagctgcgg gacaccctcg tgccggccct
      841 gactcagggc catctgcccc ccggtcttga tcatcaggca taccgtgtcg tcaccgtcgg
      901 cgagcctggt ctgcgggcgt gccgcccgcg ccgtgaatgg gctcaacatc ctggctcggc
      961 gcagaccggg cgcgatgttc atctgaatgt cgatcgggtg ccccggctcg gcgtcgatgt
     1021 ccatgccgat cgaccggtca aacagatcgc gcagggcggg caggcgccgc gccacggcaa
     1081 gttcgcatgt cgaaagccgc agcgacttcg cgcctcgctc ctcgagtctc atcagaatct
     1141 cccccgtgct acccgcgcgg cacggacatt ctgcggccac gtcggacgtg gtgtccagac
     1201 tggtcacaag tgcgagggcg agggacatct ccagttcgct tgcggcacag cggcacttcg
     1261 catacgccca agacagccgc agaggctccc gccaccatcg aaaccccttc cgttgaggag
     1321 tcgacatggt caaacccttg tgggccgcca gcaccttggt cgcggcactg gttctcgcca
     1381 atccactggc ggctcagcag agcgcgcccg cctcgcccag cgcggcgccg aacgtcaagc
     1441 gcattccact gcagcgcttc gatgtgccgg gtaccgcgtt cgaggccgtg atcggcatcg
     1501 ccgagatcgc acccagtgtc gccatcggtc gccacaccca tcctgggccc gaatcaggct
     1561 acgtgatcga gggcggattc gagttgctga tcgatggcga accgccgcgc ctgctcaaag
     1621 ccggcgactc gtacaaggtg ccgccgggag ccatccacga cgccaaggcg ggcccccaag
     1681 gcgcgaaggt aatcgccacc tacgtcgtag agaaaggcca accgttggcg gcagcagcgc
     1741 cagccgcgcc gcagtagggg cgagcgatgg tcacgcctgc accgcggctc tgccgcgggc
     1801 gtcgcgttcg tcgcatcagc cgacatgccc accttgaacc tcagggtccg ctgcaggagg
     1861 atcggcccgg cccgtgaagt gccgtgtcga ccgaagcagc ctcaagggca gacttccatg
     1921 cgccgcgtga caccgcggat gacctcgtcg ctgaaccaga agaagtagcg cgtgaccgtg
     1981 accgtggtgc tgtccttggt gcgggtcttg tactccaccg gcacgaccca gccgacatag
     2041 ccgccgctgc tgccgcccat cgggcaggcg ccgcgctgcg gggtgccgat caccagctcg
     2101 cgattcgggt cgatggcctt ggcggtggcg tcgaagtagt tggtgatggc cgactcgtgg
     2161 ttgcgcggcg ggatcgagcg caccacgcgc tcgcgtgggg cgggctgatc gctggcgcac
     2221 ccggcgacca aaagacatgc ggccaggatt gctgcggcgg ttttcgtcat ctgtggtctc
     2281 tggggtaacc cggccgtcgg ctcaccgggt ggttcgggcg tggttgtcgc aaagagagct
     2341 gcccgccaag ctctgcgacg ggcgcccggc cgattctgcc ggcaaacgcc ggacgcgccg
     2401 agtttgccat caagcgcgcc gaccgcgccg tgccgccgct tggcgtaccg cggcaggttt
     2461 cacagttctt tacgctgcag ccaatgggtc ttcaccgcgg cgccggacca tgggcgtcct
     2521 gggcaggcag cccgacccac cggagaattc aatgcatgaa gtgcaggcgg cgcggccgcg
     2581 gcggccagat tcgatgctcg tgatgcggct gatggccgtc ggtgtgttcg tggtcgctgc
     2641 ggcgggggcg acgttctccg cccacgcgca ggatcgccac gggcccggga tgggcggcgg
     2701 cgcgcacggc gacatgatga tgttcgaagg ccccgggggg cggggcgggc gcgggctcga
     2761 ccgcatgctc gacggcctca atgcgaccgc ggcacagcgc gctcagatca agcagatcgc
     2821 gatggccgcg gcgaccgacc tgaaggcgca gcgcgaagcc gaacgcgcgc tgcgcgagaa
     2881 gggcttgcag atcttcaccg cgccgacggt cgatgcggca gcggccgagt ccctgcgcca
     2941 gcagatgagc gcgcagcggg acgaggccag caagcgcagg ctgcaggcga tgctcgacat
     3001 cgccaaggtg ttgacgcccg aacagcgggc gaagatcggc gagcgcatga aagagcgcca
     3061 ggcggtgatg aaagaccgca tgcagcgcat gcagcaggaa cgcgccgagc gcgggcagcg
     3121 cccgcctgca caaaaatgag cggcgccaag gctatgctcg ccctcgacaa cgggatggcc
     3181 gcgctggcgc ttcacagcgc ggtcgtgtcc gcatcgcatc cgtccaccgg cccccattca
     3241 atgaccgctc gcctgctgct gatcgacgac gacgcccggc tgtcttccat ggtgggtgac
     3301 tacctgcgca actcggggtt cgacgttgaa accgcgggct cgctcgcggg gggccgcgag
     3361 cgcctcgcgg ccgagcactt cgacgcgctc gtgctcgacc tgatgctgcc cgacggcgac
     3421 ggcctcgacc tgtgccggga attgcgctca gagggccgcc accgccacct gccgctgctg
     3481 atgctgacgg cgcgcggcga gccgatggac cgcatcgtcg ggctcgaact cggcgccgac
     3541 gactacctgc ccaagccctt cgagccgcgc gagctgctgg cgcgcgtgaa ggccttgctg
     3601 cgccgcgccg cgccacagcc cgccggcgac gacgtgctgc gcttcgggcg cctcgaggtc
     3661 gacctcggcg cccgcgtcgc gcgcctggac ggcgcgatct gcgatctgac cggccaccag
     3721 ttcgacctgc tggtcgtgct cgcgcaaagc ccggggcgcg tgctgtcgcg cgaccagatc
     3781 atggattcgc tgaaaggcca tccgctcgaa gccttcgacc gttcgatcga cgtccacatc
     3841 tcgcgcatcc gcgcggtcat cgaggacgac ccgaagctgc cgcgccgcgt gctgaccgtg
     3901 cgcggcgccg gctacgtgtt cgcgcgcaag caggacgccg acgaagcgcg cgagtgacgc
     3961 gtgagggcgc cggccggccc cgcggcatga tgcgcgcatg aagaatctct acctgcgcat
     4021 ctacgccacc gtggtggtgg tgctgctgct gttcgccgcg gcatcgggct ggatctacca
     4081 gcgccacatg gagcaggagc gcggccgcgc cgaggccgtg ctcaccgagc gcatgggcgc
     4141 ctgggccgaa ctgatcgagc gctcgctgcc gggtcacgag atgccgcccg cggaccaggg
     4201 cgcggcgctg cgcgactggt cgcagcgtct gcgtctgccg ctcgcgctcg acagcgccga
     4261 cggcgttcgc atcgccgagt ccgaatcgtt cacacgtcgc caggccgagg gtgcggtgcg
     4321 cggtctcgcg gtgcgcctgg acgacggccg cacgctgtgg atcatgcggc cgggcctgat
     4381 gcgcagcggt ggcggccggc tcggcctggg cggcgcgccg ccaggcgtgc ggccgggcgg
     4441 cggcgaagac cgtccgccgt tcctgccgtt catcccggca gcctggcagg gcgtggggct
     4501 ggtggtcgtg ctggcgatgc tgttcgtcgc cgtctccgcc ggtgcgtacc cggtcgtgcg
     4561 ccggctcaca cgtcggctcg aggccttgaa gcagggtgtc gaacagttcg gtgcggggca
     4621 actgcaccat cgcgtccagg tcaccgggca cgacgaggtc gccgcggtcg cgtcgagctt
     4681 caatgtcgcg gcggcgcgcg tcgaggcgct ggtgcgctcg caccagtcgc tgctggcgaa
     4741 tgccagccac gagc
//
LOCUS       DATMJB010000655         3258 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_695224, whole genome shotgun sequence.
ACCESSION   DATMJB010000655 DATMJB010000000
VERSION     DATMJB010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695224"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..727)
                     /locus_tag="VNU48_13855"
     CDS             complement(50..727)
                     /locus_tag="VNU48_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWI12413.1"
                     /translation="MATPLDLEEQEQLDQLKAFWKRYGNLITWLLIAVLAAYAGWNGW
                     NWWQRDQADKSGAMFDQLDKAAQAGDAEQAARVFADMKERYPRTAFTEQGGLTTAKVQ
                     LDKGQADAALATLAWVGANAAEGEYQTIAKLRAAGILLDQKKYDEALKQLDGASAPDF
                     AALVNDRRGDVLLAQGKKDEAKAAFAKAWQAMDANVGYRRLIDAKLTALGAAPVDAFA
                     KVAEGAK"
     gene            complement(756..2072)
                     /gene="hisS"
                     /locus_tag="VNU48_13860"
     CDS             complement(756..2072)
                     /gene="hisS"
                     /locus_tag="VNU48_13860"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWI12414.1"
                     /translation="MTEPAKTRRPEPLQAVKGMNDILPPESARWEWFESKVRALMARY
                     GYANVRTPIVEPTALFVRGIGEVTDIVEKEMYSFTDAMNGDKLTLRPEATAGIVRAMI
                     EHNALYNGPMRVWSEVSVFRHERPQKGRYRQFHQIDVEALGFAGPDVDVEQILMCRAL
                     LADLGIPMDKVRLELNSLGQAGERLAHRAALIKHFEAHAELLDDDAKRRLHSNPLRIL
                     DTKNRAMQSLVEAAPQLMDFLGEASLAHLNTVRAALDAVGLEYRINPRLVRGLDYYNL
                     TVFEWTTDLLGSQGTVCGGGRYDGLFEQLGGKPTPAVGWGMGIERMLLLIEAVGGAPP
                     APAPDVYAIVPGVEALGSALLTCEALRAAGVTVLMHAAGADAWGSMKSQFKRADASGA
                     RHALIFGADELARGQVALKPLRNADAPQRTLALADAAVWAADLRTA"
     gene            complement(2149..>3258)
                     /gene="ispG"
                     /locus_tag="VNU48_13865"
                     /gene_synonym="gcpE"
     CDS             complement(2149..>3258)
                     /gene="ispG"
                     /locus_tag="VNU48_13865"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWI12415.1"
                     /translation="ELAQAGSELVRITVNTPEAAAAVPYLREQLDRMGVDVPLVGDFH
                     YNGHRLLADYPACAEALSKYRINPGNVGKGDKHDRQFAQMVEVAAKHDKAVRIGVNWG
                     SLDQELLTEMMDANAKLAEPFEPQQIMYRAILTSAVQSARRAEALGLRGDQIILSCKM
                     SGVQDLISVYRALAKRCDYALHLGLTEAGMGTKGTVASTTALGVLLQEGIGDTIRVSL
                     TPQPGEARTQEVVVALEILQSLGLRQFNPSVTACPGCGRTTSTTFQELAKQIDDYLRA
                     QMPVWKARYPGVEKMKVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFIDGEKAL
                     TLRGDNIAAEFHALVERYIDKRFGQAQAEHPVAQA"
BASE COUNT          441 a         1141 c         1124 g          552 t
ORIGIN      
        1 agtgcggcga gccagcgatt cgagacgggc acgaccacgc gacccggcgt catttcgcgc
       61 cctcggcaac ctttgcgaag gcatcgaccg gcgcggcacc cagcgccgtc agcttcgcgt
      121 cgatcaggcg ccggtagccg acgttcgcat ccatcgcctg ccaggctttc gcgaacgccg
      181 ccttggcctc gtccttcttg ccctgcgcga gcagcacatc gccgcggcgg tcgttgacca
      241 gcgcggcgaa atcgggcgcg ctcgcaccgt cgagttgctt cagcgcctcg tcgtacttct
      301 tctggtcgag caggatgccg gccgcgcgca gcttcgcgat cgtctggtac tcgccttcgg
      361 cggcattcgc gccgacccag gcgagcgtgg ccagtgcggc gtctgcctgg cccttgtcga
      421 gctgcacctt ggccgtcgtc agaccgccct gctcggtgaa cgcggtgcgc gggtagcgtt
      481 ccttcatgtc ggcgaagacg cgggcggcct gctcggcatc gccggcctgc gcggccttgt
      541 cgagctggtc gaacatcgca ccggacttgt cggcctggtc gcgctgccac cagttccagc
      601 cgttccagcc ggcgtaggcc gcgagtaccg cgatcagcag ccaggtgatc aggttgccgt
      661 agcgcttcca gaatgccttc agctggtcga gctgttcttg ttcttcgagg tcgagagggg
      721 tggccatgga caggatgttt ccagtgaagg cgggattatg cggtgcgcag gtcggcggcc
      781 cagacggccg cgtcggccag cgccagcgtg cgttgcgggg catcggcgtt acgcagcggc
      841 ttcagcgcga cctggccgcg cgccagctcg tcggcgccga agatcagcgc atggcgcgca
      901 ccgctcgcgt cggcccgctt gaactggctc ttcatgctgc cccaggcgtc ggcacccgcc
      961 gcgtgcatca gcaccgtgac gccggcagca cgcagcgcct cgcaggtcag cagcgcgctg
     1021 cccagcgcct cgacgcccgg cacgatcgcg tagacatcgg gcgcgggcgc cggcggcgca
     1081 ccgccgaccg cctcgatcaa cagcagcatg cgctcgatgc ccatgcccca gccgaccgcc
     1141 ggtgtcggct tgccgccgag ctgctcgaac aggccgtcgt agcggccgcc gccgcacacc
     1201 gtgccctgcg aaccgagcag gtcggtggtc cattcgaaga cggtcaggtt gtagtagtcg
     1261 aggccgcgca ccagccgcgg gttgatgcgg tactcgagcc cgaccgcgtc gagcgccgcg
     1321 cgcaccgtgt tcaggtgcgc gagcgaagcc tcgccgagaa agtccatcag ctgcggcgcg
     1381 gcctcgacga gcgattgcat cgcgcgattc ttcgtgtcga gaatgcgcag cgggttgctg
     1441 tgcaggcgcc gcttcgcgtc gtcgtcgagc agctcggcgt gcgcctcgaa gtgcttgatg
     1501 agcgcggcgc ggtgcgcgag gcgctccccg gcctggccga ggctgttgag ttcgaggcgg
     1561 accttgtcca tcggaatgcc gaggtcggcg agcaacgcgc ggcacatcag gatctgctcg
     1621 acatcgacgt ccggcccggc gaagccgagc gcctcgacgt cgatctggtg gaactggcgg
     1681 tagcggccct tctgcgggcg ctcgtgccgg aacaccgaga cttccgacca cacgcgcatc
     1741 ggcccgttgt acagcgcgtt gtgctcgatc atcgcgcgca cgatgccggc ggtcgcctcg
     1801 gggcgcagcg tgagcttgtc gccgttcatc gcatcggtga acgaatacat ctccttctcg
     1861 acgatgtcgg tcacctcgcc gatgccgcgc acgaacagcg ccgtcggctc gacgatcggc
     1921 gtgcgcacgt tggcgtagcc gtagcgcgcc atcagcgcgc gcaccttgga ttcgaaccac
     1981 tcccagcgcg ccgactcggg cggcaggatg tcgttcatgc ccttgaccgc ctgcagcggc
     2041 tcggggcgtc gggtcttggc gggttcagtc atggcaatgt ccgggtcaac gaggggcttc
     2101 gccgccggcg aaacctggcg gcgtcggggc gcagcgcagg ccgggagctc aggcctgcgc
     2161 gaccggatgc tcggcctgcg cctgcccgaa gcgcttgtcg atgtagcgct cgaccagcgc
     2221 gtggaactcg gccgcgatgt tgtcgccacg cagcgtcagc gccttctcgc cgtcgatgaa
     2281 caccggtgcg gcaggcgcct cgccggtgcc cggcaggctg atgccgatgt cggcgtgctt
     2341 gctctcgccg gggccgttga cgatgcagcc catcaccgcg accttcatct tctcgacacc
     2401 ggggtagcgc gccttccaga ccggcatctg cgcgcgcagg tagtcgtcga tctgcttggc
     2461 gagttcctgg aacgtggtgc tggtggtgcg gccgcagccc gggcaggcgg tcacgctcgg
     2521 gttgaactgg cgcaatccga gactctgcag gatctccagc gcgacgacca cctcctgggt
     2581 gcgcgcctcg cccggctgcg gcgtcagcga cacgcggatc gtgtcgccga tgccctcctg
     2641 cagcagcacg cccagagcgg tggtcgatgc gaccgtgccc ttggtgccca tgccggcttc
     2701 ggtcagcccc aggtgcagcg cgtagtcgca gcgcttcgcg agggcgcggt agacgctgat
     2761 caggtcctgc acgccggaca tcttgcacga caggatgatc tggtcgccgc gcagcccgag
     2821 cgcttcggcg cgccgcgccg actgcaccgc cgaggtcagg atcgcgcggt acatgatctg
     2881 ctgcggctcg aacggctccg cgagcttcgc gttcgcgtcc atcatctcgg tcagcagttc
     2941 ctggtcgagg ctgccccagt tcacgccgat gcgcaccgcc ttgtcgtgct tcgcggccac
     3001 ctcgaccatc tgcgcgaact ggcggtcgtg cttgtcgccc ttgccgacgt tcccggggtt
     3061 gatgcggtac ttgctgagcg cctcggcaca cgccggatag tcggcgagca gccggtgccc
     3121 gttgtagtgg aagtcgccga ccagcggcac gtcgacgccc atgcggtcga gctgctcgcg
     3181 caggtacggc accgccgccg ccgcctcggg cgtgttgacg gtgatgcgga cgagctccga
     3241 gccggcctgc gccagctc
//
LOCUS       DATMJB010000656         2088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_90876, whole genome shotgun sequence.
ACCESSION   DATMJB010000656 DATMJB010000000
VERSION     DATMJB010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90876"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VNU48_13870"
     CDS             complement(<1..280)
                     /locus_tag="VNU48_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003261913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI12416.1"
                     /translation="MKVYYDKDADLSLIKGKTVAIIGYGSQGHAHAQNLNDSGVKVTV
                     GLRRGGASWSKVEKAGLKVAEVAEAVKSADVVMILLPDENIADVYRNDV"
     gene            complement(324..815)
                     /gene="ilvN"
                     /locus_tag="VNU48_13875"
     CDS             complement(324..815)
                     /gene="ilvN"
                     /locus_tag="VNU48_13875"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWI12417.1"
                     /translation="MKHIIALLLENEPGALSRVVALFSARGYNIESLTVATTEDASLS
                     RMTIVTKGSEEVIEQITKHLNRLIEVVKVVDLTEGAFTGRELMLIKVRAVGKEREEMK
                     RMADIFRGRIIDVTDKSYTIELTGDTGKLDAFLEAIDRAAILETVRTGTSGIGRGERI
                     LRV"
     gene            complement(982..>2088)
                     /locus_tag="VNU48_13880"
     CDS             complement(982..>2088)
                     /locus_tag="VNU48_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWI12418.1"
                     /translation="AKRPYIYTGGGVVLGNAAAELRELADLLGFPVTNTLMGLGAMPA
                     SDPKFLGMLGMHGTYEANMTMQNCDVLLAVGARFDDRVIGNPKHFASVERKIIHIDID
                     PSSISKRVRVDIPIVGDVRDVLQELIGQVREAQLKPDKAVISAWWKQINEWRKRDCLA
                     FKTSTEVIKPQQVVQTLWELTKDSDAYITSDVGQHQMWAAQYYRFDEPRRWINSGGLG
                     TMGVGLPYAMGIKLAKPDADVFCITGEGSIQMCIQELSTCLQYKTPVKIVSLNNRYLG
                     MVRQWQQIDYGGRYSHSYMDALPDFVKLAEAYGHVGLKVEKPSDVEGALREAIRLKDR
                     TVFLDIRTDPTENVWPMVQAGKGISEMLLGSEDL"
BASE COUNT          317 a          693 c          663 g          415 t
ORIGIN      
        1 cgacgtcgtt gcgatagacg tcggcgatgt tctcgtccgg caggaggatc atcacgacgt
       61 cggccgactt caccgcctcg gcgacctcgg cgaccttcag gccggccttt tccaccttgc
      121 tccacgatgc gccgccgcgc cgcaggccca cggtcacctt cacgccgctg tcgttgagat
      181 tctgcgcatg ggcatggcct tgcgagccgt agccgatgat cgcgacggtc ttgcccttga
      241 tcaggctcag gtccgcgtcc ttgtcgtaat aaaccttcat gattgctcct tcgttgactc
      301 gttggtgggt gtgaactgaa aaactagacg cgcaggatgc gctcgccgcg accgatcccg
      361 ctggtgccgg tgcgcacggt ttcgagaatg gcggcgcggt cgatcgcctc caggaaggcg
      421 tcgagcttgc cggtgtcgcc ggtgagttcg atggtgtagc tcttgtcggt cacgtcgatg
      481 atgcggccgc ggaagatgtc ggccatgcgc ttcatctcct cgcgttcctt gccgaccgcg
      541 cggaccttga tcagcatcag ctcgcgcccg gtgaacgcgc cctcggtcag gtcgacgacc
      601 ttgacgacct cgatcagccg gttcaggtgc ttggtgatct gctcgatgac ttcttcggag
      661 cccttggtca cgatcgtcat gcgcgacagc gaggcgtcct cggtcgtcgc gacggtcagg
      721 ctctcgatgt tgtagccgcg cgccgagaac agcgcgacca cgcgcgacaa cgcgccgggc
      781 tcgttctcga gcagcagggc aatgatgtgt ttcatgtgtc gtttcagcgc gctcttcaga
      841 ccggcggcgg taaccgccgg cccttggcca cactgccttc gatgaattga gagagatgaa
      901 atcgccgaag cgcaccgcac gtcccaccgg tgtcccgggc cgggcgcggt aacgccctgc
      961 cccgcaccgg cgttcagcgc gtcacaggtc ttcggagccc agcaacattt cggaaatgcc
     1021 cttgcccgcc tgcaccatcg gccagacgtt ctcggtcggg tcggtgcgga tgtcgaggaa
     1081 cacggtgcgg tccttcaggc ggatcgcctc gcgcagcgcg ccttccacgt ccgacggctt
     1141 ctcgaccttc agcccgacgt ggccgtaggc ctcggcgagc ttgacgaagt cgggcagcgc
     1201 gtccatgtag ctgtgcgaat agcgcccgcc gtagtcgatc tgctgccact gccgcaccat
     1261 gccgaggtag cggttgttca gcgagacgat cttcaccggc gtcttgtact gcaggcaggt
     1321 cgacagctcc tggatgcaca tctggatcga gccctcgccg gtgatgcaga acacatccgc
     1381 atcgggcttc gccagcttga tgcccatcgc atacggcagg cccacgccca tcgtgcccag
     1441 gccgccggag ttgatccagc ggcgcggctc gtcgaagcgg tagtactgcg cggcccacat
     1501 ctggtgctgg ccgacgtcgg aggtgatgta ggcatcgctg tccttggtca gctcccacag
     1561 ggtctgcacc acctgctgcg gcttgatgac ctcggtgctg gtcttgaatg ccaggcagtc
     1621 gcgcttgcgc cactcgttga tctgcttcca ccacgccgag atgacggcct tgtcgggctt
     1681 gagctgcgct tccctgacct gcccgatcaa ctcctgcagc acgtccctca cgtcgccgac
     1741 gatcgggatg tcgacgcgca cgcgcttgct gatcgacgac gggtcgatgt cgatgtggat
     1801 gatcttgcgt tcgaccgacg cgaagtgctt cgggttgccg atcacgcggt cgtcgaagcg
     1861 cgcgccgacc gccagcagaa cgtcgcagtt ctgcatcgtc atgttggcct cgtaggtgcc
     1921 gtgcatgccg agcattccga ggaacttcgg gtcgctggcc ggcatcgcgc cgagccccat
     1981 cagcgtgttg gtgacgggga agcccagcag atcggccagc tcgcgcagct cggccgcggc
     2041 gttgcccagc accaccccgc cgccggtgta gatgtacggg cgcttggc
//
LOCUS       DATMJB010000657         3226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_826989, whole genome shotgun sequence.
ACCESSION   DATMJB010000657 DATMJB010000000
VERSION     DATMJB010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826989"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VNU48_13885"
     CDS             complement(<1..597)
                     /locus_tag="VNU48_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWI12419.1"
                     /translation="MTTDYEEMFELAPVSLWLEDYSALHALFTRWRAAGIADLRAHLN
                     AERARVAECSRCLKVLAVNRRTLELYGAADAPRLVASLDRVFRDEMLEAHIDELVALW
                     DGRFDFASQTVNYTLAGARLDILLRGSVLPGHRADWSRVLIAIEDISERTRTEALLAQ
                     SERYARGLFEDSPVSLWVEDFSAIKRLIDDVRARGIEDF"
     gene            complement(594..1988)
                     /locus_tag="VNU48_13890"
     CDS             complement(594..1988)
                     /locus_tag="VNU48_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HWI12420.1"
                     /translation="MASSVYLERRALLAQALRAAGGGVAVIATAPERTRNRDNDHPYR
                     HDSSFHYLTGFDEPQAWLVLHASGEAAIGCRAKSPEREIWDGLRLGPQAAPAALGVDT
                     AFDVETLDATLLEQLANQPAVWFPFDAGPEFARRIDAWLATLRGRARSGTQAPAAQRD
                     LSVLVDEMRLRKDAGELATMRRAAQISADAHARAMRFSAARFRAAPGGTVREYEIEAE
                     LLHEFRRRGAQSPAYPSIVAAGANACVLHYAASDAELRAGELCLIDAGCELDGYASDV
                     TRTFPADGRFTPAQRELYEIVLAAQQAAADATRPGARQRDAHHAAVRVLTQGMLASGL
                     LDRDVVGDVDDAIRSTAYRQFYMHGTGHWLGRDVHDVGEYLSLAEAPAEQVDAAGRTA
                     VMKPSRVLEPGMVVTLEPGLYVRPALGVPERFWNIGIRIEDDAVVTERGCELISRGVP
                     VAAHEIEALMRASP"
     gene            2081..3076
                     /locus_tag="VNU48_13895"
     CDS             2081..3076
                     /locus_tag="VNU48_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWI12421.1"
                     /translation="MRYRHLGRTGLQVSELSLGSWVTYHNQVDTSAATEMLAAAMDAG
                     VNFFDNAEVYASGQSEVVMGQAFKALKWPRLNYIVSTKFFWGLALRNDPREGNTVNRM
                     DTLNRKYLMQAIDGSLKRMGLDFIDLVYCHRPDPHTPIEETVRAMSDMITQGKALYWG
                     TSEWDAGDIRAAWDIAERHHLHKPVMEQPQYHLFHRQRVEKDYARLYEDIGLGLTTWS
                     PLASGLLTGKYRDGIPAGSRGAMPSMSYLAKGLTDERKRAAVTQLEDIAAELGCSVAQ
                     LAIAWVAKNPRVSTVITGASKLAQLQNNLGAVDVIAKLTPEVLARIDAISAPLAA"
BASE COUNT          492 a         1160 c         1148 g          426 t
ORIGIN      
        1 gaagtcctcg atgccgcgtg cgcgcacgtc gtcgatcagg cgcttgatcg cgctgaagtc
       61 ctcgacccac agcgataccg gcgaatcctc gaacaggccg cgcgcgtagc gctccgattg
      121 cgcgagaagc gcctcggtgc gcgtgcgctc gctgatgtcc tcgatcgcga tcagcacgcg
      181 cgaccagtcg gcgcggtggc cgggcagcac gctgccgcgc agcaggatgt ccaggcgcgc
      241 gccggccagc gtgtagttca cggtctggct ggcgaagtcg aagcggccgt cccacagcgc
      301 gaccagctcg tcgatgtgcg cctcgagcat ctcgtcgcgg aacacccggt cgagactggc
      361 gaccagccgc ggcgcgtcgg cggcgccgta cagctcgagc gtgcggcggt tcaccgccag
      421 caccttcagg cagcggctgc attcggcgac gcgcgcgcgc tcggcgttca ggtgcgcgcg
      481 caggtcggcg atgccggcgg cgcgccagcg cgtgaacagc gcatgcagcg cgctgtagtc
      541 ctcgagccag agcgagaccg gcgcgagttc gaacatctcc tcgtaatccg tcgtcatggc
      601 gatgcgcgca tcagcgcctc gatctcgtgc gccgcgaccg gcacgccgcg gctgatcagc
      661 tcgcagccgc gctcggtgac gaccgcgtcg tcctcgatgc ggatgccgat gttccagaag
      721 cgctcgggca cgccgagcgc cgggcgcacg tacaggcccg gctcgagcgt cacgaccatg
      781 ccgggctcga gcacgcgcga gggtttcatc acagcggtgc ggccggcggc gtcgacctgc
      841 tcggccggcg cttcggcgag cgacaggtac tcgccgacat cgtgcacgtc gcgccccagc
      901 cagtggccgg tgccgtgcat gtagaactgg cggtaggcgg tcgagcggat ggcgtcgtcg
      961 acatcgccga ccacgtcgcg gtcgagcaac ccgctggcca gcatgccctg cgtcagcacg
     1021 cgcaccgcgg cgtggtgcgc atcgcgctgg cgcgcgccgg ggcgcgtggc gtccgcggcc
     1081 gcctgctgcg ccgcgagcac gatctcgtag agctcgcgct gcgccggcgt gaagcggccg
     1141 tcggccggga aggtgcgcgt cacgtcgctg gcgtagccgt cgagttcgca gccggcatcg
     1201 atcagacaca gctcgccggc gcgcagctcg gcgtcgctcg cggcgtagtg cagcacgcag
     1261 gcgttcgcgc cggccgcgac gatcgatggg tacgcggggc tttgcgcacc gcggcggcgg
     1321 aactcgtgca gcagctcggc ctcgatctcg tattcgcgca ccgtgccgcc cggcgcggcg
     1381 cgaaagcgcg cggccgagaa gcgcatcgca cgcgcgtgtg cgtcggcgct gatctgtgcg
     1441 gcgcggcgca tcgtcgcgag ctcgccggcg tccttgcgca ggcgcatctc gtcgacgagc
     1501 accgacaggt cgcgctgcgc cgccggcgcc tgcgtgccgc tgcgcgcgcg gccgcgcagc
     1561 gtggccagcc aggcgtcgat gcggcgcgcg aactcggggc cggcgtcgaa cgggaaccac
     1621 accgcaggct ggttcgcgag ctgctcgagc agcgtcgcgt ctagggtctc gacatcgaac
     1681 gcggtgtcga cgccgagtgc cgccggcgcg gcctgcggcc ccaggcgcag cccgtcccag
     1741 atctcgcgct cggggctctt cgcgcggcag ccgatcgcgg cctcgccgct ggcgtgcagc
     1801 accagccagg cctgcggctc gtcgaagccg gtcaggtagt ggaacgagct gtcgtggcgg
     1861 tacgggtggt cgttgtcgcg gttgcgcgtg cgctcgggcg cggtcgcgat caccgcgacg
     1921 ccgccacccg ccgcacgcag cgcctgcgcg agaagcgcgc ggcgctcaag gtagacggaa
     1981 gaagccatct ccgaactgta gccgcgcgct gtgccgctgc ggcgaaccag ggcttgggct
     2041 acgcttcgcg ccttccgcac acgaaccgca ccgaatcatc atgcgctacc gacatctggg
     2101 ccgcaccggc ctgcaagtga gcgagctgtc gctcggctcc tgggtcacgt accacaacca
     2161 ggtcgacacg agcgcagcca ccgagatgct ggccgccgcg atggacgccg gcgtcaactt
     2221 cttcgacaac gccgaggtct acgcctcggg ccagagcgag gtcgtgatgg gccaggcctt
     2281 caaggcgctg aagtggccgc gcttgaacta catcgtctcg accaagttct tctggggtct
     2341 cgctcttcgc aatgacccgc gggaaggcaa caccgtcaac cggatggaca cgctgaaccg
     2401 caagtacctg atgcaggcga tcgacgggtc gctcaagcgc atggggctgg acttcatcga
     2461 cctggtctac tgccaccgcc ccgacccgca cacgccgatc gaggagacgg tgcgcgcgat
     2521 gagcgacatg atcacccagg gcaaggcgct gtactggggc accagcgaat gggacgccgg
     2581 tgacatccgc gccgcctggg acatcgccga gcgccaccac ctgcacaagc cggtgatgga
     2641 gcagccgcag taccacctgt tccaccgcca gcgcgtcgag aaggactacg cgcgcctgta
     2701 cgaggacatc ggcctgggcc tgacgacctg gagcccactg gccagcggcc tgctgaccgg
     2761 caagtaccgc gacggcatcc cggccggcag ccgcggcgcg atgcccagca tgagctacct
     2821 ggcgaaaggc ctgaccgacg aacgcaagcg cgccgcggtc acgcagctcg aagacattgc
     2881 cgccgagctc ggctgcagcg tcgcgcaact cgcgatcgcg tgggtcgcga agaacccgcg
     2941 cgtcagcacg gtgatcaccg gcgcctcgaa gctggcgcag ctgcagaaca acctcggcgc
     3001 ggtcgacgtg atcgcgaaac tgacacccga ggtgctggcg cggatcgacg cgatcagcgc
     3061 gccgctggcg gcctgagcac ggctcagtcg cggcgctgcg ccaggcgcca gccggcgtac
     3121 agcgcggccg ccaggccgac cagcctgagc aggcgccgcc agccgccgcg cccgcgcagc
     3181 accggcacga cgccgccgag cagcgccgcg gcgacgcggt agcggc
//
LOCUS       DATMJB010000658         8381 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_552361, whole genome shotgun sequence.
ACCESSION   DATMJB010000658 DATMJB010000000
VERSION     DATMJB010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 8381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8381
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552361"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1994
                     /locus_tag="VNU48_13900"
     CDS             <1..1994
                     /locus_tag="VNU48_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWI12422.1"
                     /translation="LKPSNILVDAAGRARVMDFGIAARLNDPGNLDQIVGTPGYMSPE
                     ATKGGLPTAAMDVFSAGIVLVELLSGQKLIAERDPYRAIYRAAHEDLVLPAGLPSTVD
                     DGLRNILLRAIARDATRRPASAALLRSALQDWLVPVAATEPTAAANSGTLDFLLRRMR
                     HKSDFPALSDSVARIQRVANSENESLASLSSEILKDVALTNKLLRLVNTAHFSNAGGN
                     ISTVSRAVALIGFAGIRNLAMSLVLLEHMHDKAHANQLKEEFLRALMAGMLASELGSL
                     TRAGEEAFIGAMFQNLGRLLTEYYFPEEARQVRGIVAAAGQGDAPAVSEDAAAMSVLG
                     LSFEALGLGVAKAWGLPSTLQACMRKPGGEPPAREPADAIERLRWLTLAANQITDALL
                     NAEPEQGQARVAAVAKRCARSLGVSPAAVQAAAETAQQRLVQMAQAMNLQVGLKSPAR
                     RLFPTPTPGGTPAEVQEHDSLVPHSLQATITVEQQQAPRTPLENAAEVLAAGIQDITN
                     TMVENFKLNDVLRMILETMYRALGFRRIVFCLRDAKTETLTGRFGLGDGAETVAKCFK
                     VPLKASGDLFAAVCIKGADTLITDATVAHIGARLPAWYRAQVNAPAFLLLPMQMKGAP
                     FALIYADKAEPGGIALGEKELSLLRTLRNQAVMAFKQVA"
     gene            complement(2023..2226)
                     /locus_tag="VNU48_13905"
     CDS             complement(2023..2226)
                     /locus_tag="VNU48_13905"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HWI12423.1"
                     /translation="MTASGVSFDSIRAGLSASASDATGGNETFFIADAGGPIAAVPEP
                     QTYALLLAGLAGIGFVARRRRSD"
     gene            2365..2598
                     /locus_tag="VNU48_13910"
     CDS             2365..2598
                     /locus_tag="VNU48_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12424.1"
                     /translation="MLTAVLIVIAVVAAWLLGDWLLSRRDERLQRGRAAWLEAGPNRS
                     FHSTAWDDTVPPLEAHDEPADDPRKPGRARAAV"
     gene            2693..3022
                     /locus_tag="VNU48_13915"
     CDS             2693..3022
                     /locus_tag="VNU48_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWI12425.1"
                     /translation="MTEEQVIGALAALAHPVRLKVFRALVVSGPAGLTPGVMQAGLAV
                     PGTTLSFHLKELAGAGLATAERASRNLVYRADYAQMNDLLGYLTENCCQGAACAIDAP
                     SASCQCR"
     gene            3061..3756
                     /locus_tag="VNU48_13920"
     CDS             3061..3756
                     /locus_tag="VNU48_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HWI12426.1"
                     /translation="MPALSRKIGAEALGTALLLAVVIGSGVMGERLSGGNTALALLAN
                     ALATVGGLYVLIEVFAPVSGAHFNPAVSLALAARGAFPRRLLLPFIAAQLAGAVLGAW
                     LANAMFDLDLLQFSTKSRGGGGQWIAEVTATFGLLLVVLRAPPARGSAMVAAYIGAAY
                     WFTASTSFANPAATFGRMFSDSFAGIAPASAPGFIVCELAGAGLAVVVHQLLGEATPP
                     PESSGIRGTKHHD"
     gene            3749..4174
                     /gene="arsC"
                     /locus_tag="VNU48_13925"
     CDS             3749..4174
                     /gene="arsC"
                     /locus_tag="VNU48_13925"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835100.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HWI12427.1"
                     /translation="MTEVVIYHNPACGTSRNTLALIRHAGIEPTIVEYLSSPPSKEKL
                     RELVAAMGQPVRALLREKGTPYAALGLADPRWTDDQLLDFIVAQPILMNRPVVSTPLG
                     TKLCRPSEEVLELLPVAKLAPFCKEDGERIEDSGRRRAP"
     gene            complement(4198..4294)
                     /gene="ffs"
                     /locus_tag="VNU48_13930"
     ncRNA           complement(4198..4294)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VNU48_13930"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(4343..5893)
                     /locus_tag="VNU48_13935"
     CDS             complement(4343..5893)
                     /locus_tag="VNU48_13935"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HWI12428.1"
                     /translation="MIAALLVALLSGCAGVLPKVERTPSTTLVAAPDAPLAQVAQHAA
                     IPAGQSGVWPLLQGSYALDARLAMIENARRSLDLQYYLIADDSTGRPLLRALRDAAAR
                     GVRVRLLVDDLYTIDLDRLLLGLAATPNAEVRLFNPFPTARDSSLGRLLALGLDFKRL
                     NHRMHNKLFIADGVMAVIGGRNLADEYFLRGAQANFIDFDVMLTGAVVPELGRWFDHY
                     WNSTQVYPVRDIARAAGRAPLDADARATFDAVTRDDPRPEVPIAPDWFGAPAFSTGLA
                     QHSLQFLAAEAFAFADSPDKIVPVNLSVASAETLTHRFLEILGESHAEVVLFSPYFVP
                     GKEALARIAALRDDGVAVRVVTNSLAVSDEPLVSIGLQGHQVEMLKMGVELYELSSNR
                     LKLDRTFKGLLGTSTGRLHAKIGFLDRRLVLVGSMNLDPRSSSINTEIGVRIASPKLA
                     EMVLAAVKLDSLAGVYQVKLARSGTGVRWVAADADSPEELDVDPDTSLWQRVRLMMLS
                     LFVPESQL"
     gene            complement(5997..7115)
                     /locus_tag="VNU48_13940"
     CDS             complement(5997..7115)
                     /locus_tag="VNU48_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HWI12429.1"
                     /translation="MIPTTPSATLSYEQAGVDYDLIDPLKVSAQRAAAATAVHLAGHG
                     FSEVAASRGESAYVVDVGPFYLASIVECLGSKALVADEMRQLTGKSYYDGIAQDTIAM
                     AINDLITVGATPLVVQAYWAAGGSGWFADAQRSQALVAGWKRACDTCRVAWGGGETPA
                     LAGIVADGRIDLAAACTGLVNPKSRLSLGEQLTPGDAIVLLASSGIHANGLSLARKLV
                     ERLPQGYLTEVEPGLSYGDALLAPTVLYSPVTEALFAAGITPRYCANITGHGWRKLLR
                     HPAAQRYRIHTVPAVPAVLKFIQHHAQQDDREAYSTLNMGAGFAIFVAAGDAQRTVEV
                     ARAQGVEALIAGAVEAGPKELLIEPLDIRFGDDDLQLR"
     gene            7215..>8381
                     /gene="dnaX"
                     /locus_tag="VNU48_13945"
     CDS             7215..>8381
                     /gene="dnaX"
                     /locus_tag="VNU48_13945"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWI12430.1"
                     /translation="MSYVVLARKYRPPNFEALVGQEHVVQALANALTQQRLHHAYLFT
                     GTRGVGKTTIARILAKSLNCTGTDGQGGITARPCGVCDACRDIDAGRFIDYVELDAAS
                     NRGVDEISELLDQAVYKPVLGRFKVYMIDEVHMLSTTAFNAMLKTLEEPPEYLKFVLA
                     TTDPQKVPPTVLSRCLQFNLRPMAPQTVQEHLATVLAQERIGAEPGALRLLARAARGS
                     MRDALSLTDQAIAFGAGTLAEDGVRRMLGAVDRGHAVRIVEAVAAADGAALIGVVDAL
                     RGLGLSAAGTLEEMAALLQQMAVLQAVPDAADPGDPDAPQAARLAALFAADETQLLYS
                     IALHGRAELGLAPDEYSGLVMVLLRMLAFRPKGERAQAGAKPARVAAADGAALIG"
BASE COUNT         1238 a         2936 c         3000 g         1207 t
ORIGIN      
        1 acttgaagcc gtcgaacatc ctggtcgatg ccgcgggccg cgcccgcgtg atggacttcg
       61 gcatcgccgc gcgcctgaac gatcccggca acctggacca gatcgtcggc acgcccggct
      121 acatgtcgcc cgaggcgacc aagggcgggc tgccgactgc cgcgatggac gtgttctccg
      181 ccggcatcgt gctggtcgag ctgctgagcg gccagaagct gatcgccgag cgcgacccgt
      241 accgcgcgat ctatcgcgcc gcacatgaag acctggtgct gccggccggt ctgcccagca
      301 cggtcgacga cggcctgcgc aacatcctgc tgcgcgccat cgcccgcgac gcgacgcgcc
      361 gcccggccag cgccgcgctg ttgcgcagcg cgctgcagga ctggctggtg ccggtggccg
      421 ccaccgagcc gacggccgcg gccaacagcg gcacgctcga cttcctgctg cgccgcatgc
      481 gccacaagag cgacttcccg gcgctgtcgg actcggtcgc gcgcatccag cgcgtcgcga
      541 actccgagaa cgagagcctg gcgagtctgt cgagcgagat cctgaaggac gtggcgctga
      601 ccaacaagct gctgcgcttg gtcaacacgg cgcacttctc gaacgccggc ggcaatatca
      661 gcacggtctc gcgcgcggtc gcgctgatcg gctttgccgg catccgcaac ctggcgatga
      721 gcctggtgct gctcgagcac atgcacgaca aggcgcatgc gaaccagctg aaggaggaat
      781 tcctgcgcgc gctgatggcc ggcatgctgg ccagcgaact cggcagcctg acgcgggccg
      841 gcgaagaggc cttcatcggc gcgatgttcc agaacctcgg tcggctcctg accgagtact
      901 actttcccga agaggcgcgc caggtgcgcg gcatcgtcgc cgcggccggg cagggcgatg
      961 cgccggcggt cagcgaagac gccgccgcga tgagcgtgct cgggctgagc ttcgaggcgc
     1021 tcggcctggg tgtggcgaag gcctggggcc tgccgtcgac actgcaggcc tgcatgcgca
     1081 agccgggcgg cgaaccgccg gcgcgcgaac cggccgacgc gatcgagcgg ctgcgctggc
     1141 tgacgctggc ggcgaaccag atcaccgatg cgttgctcaa cgccgagccc gagcaaggcc
     1201 aggcccgcgt cgccgcggtc gcgaagcgct gtgcgcgcag tctcggcgtg agcccggccg
     1261 cggtgcaggc cgcggccgag accgcccagc agcggctggt gcagatggcg caggcgatga
     1321 acctgcaggt cggcctgaag tcgccggcgc gccgcctgtt cccaaccccg acgccgggcg
     1381 gcacgccggc cgaagttcag gagcacgatt cgctggtgcc gcactcgctg caggcgacga
     1441 tcacggtcga gcagcagcag gcgccgcgca cgccgctgga gaacgccgcc gaggtgctgg
     1501 ccgcgggcat ccaggacatc accaacacga tggtcgagaa cttcaagctc aacgacgtgc
     1561 tgcgcatgat cctcgagacc atgtaccgcg cgctcggctt ccggcgcatc gtgttctgcc
     1621 tgcgcgacgc gaagaccgag acgctcaccg gccgcttcgg cctgggcgat ggcgccgaga
     1681 cggtggccaa gtgcttcaag gtgcccttga aggccagcgg cgacctgttc gccgcggtct
     1741 gcatcaaggg cgccgacacc ttgatcaccg acgccaccgt ggcccacatc ggcgcgcggc
     1801 tgccggcctg gtaccgcgcg caggtcaacg cgccggcgtt cctgctgctg ccgatgcaaa
     1861 tgaagggcgc gccgttcgcg ctgatctatg ccgacaaggc cgagcccggc ggcatcgcgc
     1921 tgggcgagaa ggaactgtcg ctgctgcgca cgctgcgcaa ccaggcggtg atggcgttca
     1981 agcaggtcgc ctgagcgcca cgcaccgcgt ggccccgggg cctcaatcgc tgcgacggcg
     2041 ccgcgcgacg aagccgatgc cggccagccc cgccagcagc agcgcgtagg tctggggttc
     2101 cggcaccgca gcgatcgggc cgccggcgtc ggcgatgaag aaggtctcgt tgccgccggt
     2161 cgcgtcgctc gcgctggccg acaggccagc ccggatcgaa tcgaacgaga cgcccgacgc
     2221 cgtcaccgcc gccgtgaacg cgcagttcga gcgtggcggt cgcgtcgacc ttcattcgag
     2281 gggtgctggg ccggccgagg tagcgtagat tcgcaccaga ccgtcaaccg atttgcaagt
     2341 tcggcggcac cccaggagcc ccccatgctg accgccgttc tgatcgtcat cgccgtcgtt
     2401 gcggcctggt tgctcgggga ctggctgctg tctcggcgcg acgaacgcct gcagcgcggc
     2461 cgcgcggcct ggctggaggc cggcccgaac cgcagcttcc actcgaccgc gtgggacgac
     2521 accgtgccgc cgctggaggc gcacgacgag ccggccgacg acccccgcaa gccggggcgg
     2581 gcgcgcgccg cggtctgatc gcggcccggt gaccgggtgc cgagccggtc gatgagccat
     2641 tgccattgcc cacactcaac gactattcga gaatagtggt acagtcgtcg gcatgacgga
     2701 agaacaagtg attggcgcgc tcgcggcgct cgcgcatccg gttcgcttga aggtgttcag
     2761 ggcgctggtc gtgtccggcc ccgcggggct gacgccgggg gtgatgcaag caggcctggc
     2821 cgtgccgggg acgaccctgt ccttccacct gaaggaactc gccggtgccg gcctggccac
     2881 cgccgagcgc gcgagccgca acctggtcta ccgggcggac tacgcccaga tgaacgacct
     2941 gctcggctac ctcaccgaaa actgctgtca gggcgccgcc tgcgccatcg atgcgccgtc
     3001 ggcatcctgc cagtgccgat gacaccactc gccggccccg ggcgcgacgg ggcctcgccc
     3061 atgccggcac tgtcgcgcaa gatcggcgcc gaagcgctgg gcaccgcctt gctgctggcc
     3121 gtcgtgatcg gctccggcgt gatgggcgag cgcctgtcgg gcggcaacac ggctctggcg
     3181 ctgctggcga atgcgctggc cacggtcggc gggctctacg tgctgatcga agtctttgcg
     3241 cccgtcagcg gggcccattt caatccggcg gtctcgctcg cgttggccgc gcgaggcgcg
     3301 ttcccgcggc ggctgctgct tccgttcatc gcggcccagc tggcgggcgc cgtgctcggt
     3361 gcctggctgg cgaacgcgat gttcgacctg gaccttcttc agttctcgac caaatcgcgc
     3421 ggcggcggcg gccagtggat cgccgaagtc accgcgacct tcgggctgct gctggtggtg
     3481 ctgcgcgcgc cgccggcgcg cgggtccgcg atggtggcgg cctacatcgg cgccgcgtac
     3541 tggttcaccg cgtcgacctc gttcgcgaac ccggcggcga ccttcgggcg catgttcagc
     3601 gacagctttg ccgggatcgc gccggcgagc gcgccgggct tcatcgtctg cgaactcgcc
     3661 ggcgccgggc tggccgtggt cgttcaccaa ctgctcggcg aggcaacgcc gccccccgag
     3721 tcctccggca tccggggcac caaacaccat gactgaggtc gtcatctatc acaaccccgc
     3781 gtgcggcacc tcgcgcaaca cgctggcgct gatccgacat gccggcatcg agccgacgat
     3841 cgtcgagtac ctgagctcgc cgccgtcgaa agagaaactg cgcgaactcg tcgccgcgat
     3901 gggccagccg gtgcgcgcgc tgctgcgcga gaaggggacg ccctatgccg cactcggcct
     3961 ggcggacccg cgctggaccg acgaccagct cctggacttc atcgtcgcgc agccgatcct
     4021 gatgaaccgg ccggtggtgt cgacgccgct gggcaccaag ctgtgccggc cctcggaaga
     4081 ggttctggaa ctgctgccgg tggccaagct cgcgcccttt tgcaaggaag acggcgagcg
     4141 catcgaggac tcgggccggc gccgcgcgcc ttgagcacag ggctggcgcg gtagaagggt
     4201 gacgagcctg accccggcac tggttgcaac ggttggggct gcttcgttcc cgacctgacc
     4261 cggttggccg cgcttccatg cggggaggcc cgtcaccgcc aattctaggc gacgcagcga
     4321 tggccgcacg cccaggcggt cttcagagct ggctttcggg cacgaacaac gacagcatca
     4381 tcaggcgcac gcgctgccac aggctggtgt cggggtcgac atcgagttcc tcggggctgt
     4441 cggcatcggc cgccacccag cgcacgccgg tgccgctgcg cgccagcttg acctggtaca
     4501 caccggccag cgaatcgagc ttgaccgcgg ccagcaccat ctcggcgagt ttcgggctgg
     4561 cgatgcgcac gccgatctcg gtgttgatgc tggacgagcg cgggtccaga ttcatcgagc
     4621 cgacgagcac gagacgacgg tccaggaagc cgatcttcgc gtgcagtcgg cccgtcgagg
     4681 tgccgagcaa acccttgaat gtgcggtcca gcttgagccg gttggaactc agttcgtaca
     4741 gctcgacgcc catcttcagc atctcgacct ggtggccctg gaggccgatg ctgaccagcg
     4801 gctcgtcgct gacggccagc gagttcgtga cgacacggac ggccacgccg tcgtcgcgca
     4861 gcgcggcgat gcgtgccagc gcctccttgc ccgggacgaa atagggcgag aacagcacca
     4921 cctcggcgtg cgactcaccg agaatctcca ggaaacggtg cgtcagcgtt tcggcgctgg
     4981 ccacgctgag gttcaccggg acaatcttgt ccggcgagtc ggcgaatgcg aaggcctcgg
     5041 ccgcgaggaa ctgcaggctg tgctgcgcca agccggtgct gaacgccggt gcgccgaacc
     5101 agtcgggcgc gatcggcacc tcggggcggg ggtcgtcgcg cgtcaccgcg tcgaaggtcg
     5161 cccgggcgtc cgcgtccagg ggtgcgcggc cggcggcgcg cgcgatgtcc cgcaccgggt
     5221 agacctgggt gctgttccag tagtgatcga accagcggcc gagctcgggc accacggcac
     5281 cggtcagcat cacgtcgaag tcgatgaagt tggcctgcgc gccgcgcagg aagtattcgt
     5341 cggcaaggtt gcgcccgccg atcaccgcca tcacgccgtc ggcgatgaac agcttgttgt
     5401 gcatgcgatg gttcaggcgc ttgaagtcca ggccgagcgc gagcagccgg cccagcgacg
     5461 agtcgcgcgc cgtcgggaag gggttgaaca ggcgcacctc ggcgttcggc gtggccgcca
     5521 ggccgagcag cagccggtcg aggtcgatcg tgtacaggtc gtcgaccagc aggcgcacgc
     5581 gcacgccgcg cgcggcggcg tcgcgcagcg cgcgcagcag aggccgcccg gtcgagtcgt
     5641 cggcgatcag gtagtactgc aggtcgagcg agcggcgcgc gttctcgatc atcgccaggc
     5701 gcgcgtcgag cgcgtagctg ccttgcagca gcggccacac gccggactga ccggcgggta
     5761 tcgcggcgtg ctgcgcgacc tgggccagcg gcgcgtcggg tgctgcgacc agcgtcgtcg
     5821 atggtgtgcg ctcgaccttc ggcagcacgc cggcgcagcc gctcagaagc gcgaccagca
     5881 gagcggcgat cacgagtcgc aggccgacgg gccgccgcgc cgggccgccc caagcaaggc
     5941 ccgaccctcc cggagggtcg accgccgtac gcggcgggcg gggtgtcgtc atgacttcag
     6001 cgcagctgca gatcgtcgtc gccgaagcgg atgtccagcg gctcgatcag caactccttc
     6061 ggccccgcct cgaccgcgcc ggcgatcagc gcctcgacgc cctgcgcgcg cgcgacctcg
     6121 acggtgcgct gcgcatcgcc ggccgcgacg aagatcgcga agccggcgcc catgttgagc
     6181 gtgctgtagg cctcgcggtc gtcctgctgg gcgtgatgct ggatgaactt cagcaccgcc
     6241 ggcaccgccg gcacggtgtg gatgcggtag cgctgggcgg ccgggtgccg cagcagcttg
     6301 cgccagccgt gcccggtgat gttggcgcag tagcgcggtg tgataccggc cgcgaacagc
     6361 gcctcggtga ccggcgagta cagcacggtg ggcgcgagca gcgcgtcgcc gtagctcagg
     6421 ccgggctcga cctcggtcag gtagccctgc ggcaaccgct cgaccagctt gcgcgccagg
     6481 ctcaggccgt tcgcgtggat gccgctggag gcgagcagca cgattgcgtc gccgggcgtc
     6541 agctgctcgc cgagcgacag gcgcgacttc gggttgacca ggccggtgca ggccgcggcg
     6601 aggtcgatgc gtccgtcggc gacgatgccg gccagcgccg gggtctcgcc gccgccccag
     6661 gccacgcggc aggtgtcgca ggcgcgcttc cagccggcca ccagcgcctg cgagcgctgc
     6721 gcgtcggcga accagcccga gccgccggcg gcccagtagg cctgcacgac cagcggcgtg
     6781 gcaccgaccg tgatcaggtc gttgatggcc atcgcgatcg tgtcctgggc gatgccgtcg
     6841 tagtagctct tgccggtgag ctggcgcatt tcgtcggcga ccagcgcctt cgagcccagg
     6901 cattcgacga tgctggcgag gtagaacggg ccgacatcca ccacataggc cgattcgccc
     6961 cgcgacgccg cgacctcgct gaagccgtgg ccggcgaggt ggacggcggt ggcggccgcg
     7021 gcgcgctgcg cgctgacctt cagcgggtcg atcaggtcgt agtcgacgcc cgcttgttcg
     7081 tagctgagcg tggcagacgg ggtcgtggga atcatcgggc tggggggcaa tgagcagtgg
     7141 ccggattatc cggcaccgcc gaccgatgga ctcatggggc agggggctgc gatcctcccc
     7201 cgtagaatga atccatgtcc tatgtcgttc tcgcccgcaa ataccggccg cccaatttcg
     7261 aggcgctggt gggccaggag cacgtggtgc aggcgctggc gaacgcgctg acgcagcagc
     7321 gcctgcacca tgcctacctg ttcaccggca cgcgcggcgt cggcaagacg acgatcgcgc
     7381 gcatcctcgc gaagtcgctg aactgtaccg gcaccgacgg ccagggcggc atcacggcgc
     7441 ggccctgcgg cgtctgcgat gcctgccgcg acatcgacgc gggccgcttc atcgactatg
     7501 tcgaactcga cgccgcctcg aaccgcggcg tcgacgagat ctcggagctg ctcgaccagg
     7561 ccgtctacaa gccggtgctg ggccgcttca aggtctacat gatcgacgaa gtgcacatgc
     7621 tgtcgacgac cgcgttcaac gcgatgctca agacgctgga agagccgccc gagtacctca
     7681 agttcgtgct cgcgaccacc gacccgcaga aggtgccgcc gacggtgctg tcgcgctgcc
     7741 tgcagttcaa cctgcggccg atggcgccgc agaccgtgca ggagcacctc gcgaccgtgc
     7801 tcgcgcagga gcgcatcggc gccgagcccg gcgcgctgcg cctgctcgcc cgcgcggcgc
     7861 gcggctcgat gcgcgacgcg ctgtcgctga ccgaccaggc gatcgccttc ggtgccggca
     7921 cgctggccga agacggcgtg cgccggatgc tcggcgcggt cgatcgcggt cacgcggtgc
     7981 gcatcgtcga ggccgtcgct gccgccgatg gcgccgcgct gatcggcgtg gtcgacgcgc
     8041 tgcgcggcct gggcctgtcg gcggccggca cgctcgaaga gatggccgcg ctgctgcagc
     8101 agatggcggt gctgcaggcc gtgcccgatg cggccgaccc gggcgatccc gacgccccgc
     8161 aggcggcacg gctggcggcg ctgttcgccg ccgacgagac ccagctgctc tacagcatcg
     8221 cgctgcatgg ccgcgccgag ctcggcctgg cgcccgacga atacagcggc ctggtgatgg
     8281 tgctgctgcg catgctcgcg ttccggccga agggcgagcg cgcgcaggcg ggcgcgaagc
     8341 cggcgcgcgt cgctgccgcc gatggcgccg cgctgatcgg c
//
LOCUS       DATMJB010000659         3902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_506581, whole genome shotgun sequence.
ACCESSION   DATMJB010000659 DATMJB010000000
VERSION     DATMJB010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506581"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..338)
                     /locus_tag="VNU48_13950"
     CDS             complement(45..338)
                     /locus_tag="VNU48_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HWI12431.1"
                     /translation="MKHLMTVLALSVACLSTSAFAADAASAPMGKQQSKMGECNASAG
                     DKKGDERKAFMKECLSAKKMSQQEKMKSCNADPKAKAAKGDERKAFMKECLSK"
     gene            complement(468..1832)
                     /locus_tag="VNU48_13955"
     CDS             complement(468..1832)
                     /locus_tag="VNU48_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HWI12432.1"
                     /translation="MKTVPTPFEPSPPEAPALPRHIGKYRVLSRLGEGATAEVFLCHD
                     DFKGIDVAIKRVRANVNGDARDGRFSERFFAAEAALVGRLHHPNVVQILDAVADPVAP
                     YLVMEYVAGSTLRPHCRPDQLLALELVVEIGFKCAMALGYVYRQGLIHRDVKPANLLA
                     VLNATGAITDVKISDFGSVLNLSADTTQIYRVGSLAYMAPEQLDGGTLDCRADMYSLG
                     AVLYHLIAGRPPFDGQVQSAMMHQIYHAKPAALTGLREGVSPGLDAMIQRALAKDPAQ
                     RYPDWEAFAQALSALITNQEVPRGQLQGVLDSERFNLLRSLDFFARFGDVELWEVVHR
                     AKWQRFHFGHALYRKGEEGNSFHILAQGEVEVFRDGKRVAKLGSGTSIGEMAYLAPNP
                     ELKRHSTDVIVSQPATTISFTPETLVQVSAGCRHLFDEAFIRVLVRRLHVAHEALAHP
                     RRIL"
     gene            1942..3699
                     /gene="argS"
                     /locus_tag="VNU48_13960"
     CDS             1942..3699
                     /gene="argS"
                     /locus_tag="VNU48_13960"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWI12433.1"
                     /translation="MIQAKQDLVEALRAAIVEVAPGATLAPTFESPKQAAHGDLACTA
                     AMPLAKALKKNPREVAAALVEALNRQAAVRRWVDALEIAGPGFINLRLKAAAKQAVVA
                     EVLAAADAFGRQPPKADRVLVEFVSANPTGPLHLGHARQAALGDAICNLFETQGWQVS
                     REFYYNDAGVQIATLAASVQARLQGLSPGDAQWPAAAYNGEYIADIAADFRAKKTVRA
                     DDRAFTASGDAGDLDGIRQFAVAYLRHEQDLDLQAFGVRFDHYFLESSLYSSGQVDAT
                     VQRLIASGKTFEEGGALWLRTTDYGDDKDRVMRKSAPAGADASSANASVYTYFVPDVA
                     YHVNKFERGFTKAINIQGGDHHGTIARVRAGVQAAGAGVPAGYPEYVLHKMVTVMKGG
                     QEVKISKRAGSYVTLRDLIDWTSRDAVRFFLLSRKADSEFTFDVDLALKRNDENPVFY
                     VQYAHARICSVIRNFADQGGQLAGAPDLALLSAPTEAALMLRLAEFPAVLANAATSLA
                     PHDLTFYLRDLAGAFHSYYGAERVLGDDAALTRARIALLVATRQVIRNALAVLGVSAP
                     ERMDRDVEPKPEPASASTP"
     gene            3696..>3902
                     /locus_tag="VNU48_13965"
     CDS             3696..>3902
                     /locus_tag="VNU48_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12434.1"
                     /translation="MTSTQQQRGGFAVGLIIGLLIGLALALGVALYVTKVPIPFVNKV
                     PQRTADQDAAEVEKNKNWDPNSPLY"
BASE COUNT          633 a         1340 c         1325 g          604 t
ORIGIN      
        1 tcgggggatc ggctgacgta ctcgtcagcc gaggggccgt catctcactt gctgaggcat
       61 tccttcatga aggccttgcg ctcgtcgccc ttcgcagcct tggccttcgg gtcggcgttg
      121 caggacttca tcttttcctg ctggctcatc ttcttggccg acaggcattc cttcatgaag
      181 gccttgcgct cgtcgccctt cttgtcgccg gcgctcgcgt tgcattcgcc catcttgctt
      241 tgctgcttgc ccatcggcgc gctggcggcg tcggctgcga aggcggaggt cgacagacag
      301 gccacggaca gggcgagaac ggtcatcaga tgtttcatac ggagctcctg gggagttttg
      361 tcgaagtgag gctcagcgcc ggcacggatt gaagcacgcg ccccagggcg ccagcaagga
      421 ctcaacgtgt cagtcggtct cccggtgcac acgcgaccag ggccgcgtca cagaatgcgc
      481 cgcgggtgcg cgagcgcctc gtgcgcgaca tgcagccgcc gcaccagcac gcggatgaag
      541 gcctcgtcga acaggtggcg gcagccggcg ctgacctgca ccagcgtctc gggcgtgaac
      601 gagatcgtgg tcgccggttg ggagacgatc acatcggtgc tgtggcgctt cagctcgggg
      661 ttgggcgcga ggtaggccat ctcgccgatg gaggtgccgc tgccgagctt ggcgacccgc
      721 ttgccgtcgc ggaacacctc gacctcgccc tgcgcgagga tgtgaaagct gttgccttcc
      781 tcgcccttgc ggtacagcgc gtggccgaaa tggaagcgct gccacttcgc gcggtgcacc
      841 acttcccaga gctcgacgtc gccgaagcgg gcgaagaaat cgagcgagcg caacaggttg
      901 aagcgctccg agtcgagcac gccctgcagc tggccgcgcg gcacttcctg gttcgtgatc
      961 agcgccgaca gtgcctgcgc gaacgcctcc cagtcggggt agcgttgcgc cggatccttt
     1021 gccagcgcgc gctggatcat cgcgtccaag ccggggctga cgccctcgcg cagcccggtc
     1081 agcgcggccg gcttggcgtg atagatctga tgcatcattg ccgactgcac ctgcccgtcg
     1141 aagggtgggc gcccggcgat caggtggtag agcacggcgc ccagcgagta catgtcggcg
     1201 cggcagtcga gcgtgccacc gtcgagctgc tcgggcgcca tgtaggccag cgagccgacg
     1261 cggtagatct gcgtcgtgtc ggccgacagg ttcagcacgc tgccgaaatc gctgatcttc
     1321 acgtcggtga tcgcaccggt cgcgttcaac accgcgagca gattcgccgg cttcacgtcg
     1381 cggtggatca ggccttggcg gtaaacgtag cccaaagcca tcgcgcactt gaagccgatt
     1441 tcgacgacga gctcgagcgc gagcagttga tcggggcggc agtgcgggcg cagcgtgctg
     1501 ccggccacgt attccatcac gagatagggc gcgaccgggt cggcgaccgc gtccaggatc
     1561 tgcacgacgt tcgggtggtg cagccgcccg accaacgccg cttcggcggc gaagaagcgc
     1621 tccgaaaagc gcccgtcccg cgcgtcgccg ttgacgttgg cgcgcacgcg cttgatcgcg
     1681 acgtcgatgc ccttgaagtc gtcgtggcac aggaagacct cggccgtcgc gccctcgccc
     1741 aggcgcgaca acacccggta cttgccgatg tgccggggca gcgcgggcgc ctccggcggg
     1801 ctcggctcga acggcgtggg gacggttttc atggaagagc gcacagaccg gcatcttcgc
     1861 acccgggcaa tgccggtgtc gtcccgaaca gccgggcaaa gcgcaactat tcccgaacgg
     1921 gtgcccccgt agaatccctg aatgatccaa gccaaacagg acctcgtcga ggcgcttcgc
     1981 gcggcgatcg tcgaggtcgc gccgggcgcc acgctggccc cgacgttcga atcgccaaaa
     2041 caggccgcgc acggcgacct ggcctgcact gcggcgatgc cgctcgcgaa ggcgctcaag
     2101 aagaacccgc gcgaggttgc ggccgcactg gtcgaggcgt tgaaccggca agcggcggtg
     2161 cggcgctggg tcgacgcgct cgagatcgcc ggccccggct tcatcaacct gcgcctgaag
     2221 gcggccgcca agcaggccgt cgtggccgaa gtgctggccg cggccgacgc cttcggccgc
     2281 cagccgccga aggccgatcg cgtgctggtc gagttcgtct cggcgaatcc gaccggcccg
     2341 ctgcacctgg gccatgcgcg ccaggccgcg ctcggcgacg cgatctgcaa cctgttcgag
     2401 acccagggct ggcaggtcag ccgcgagttc tactacaacg acgccggcgt gcagatcgcc
     2461 acgctcgcgg cgtcggtgca ggcgcggctg cagggcctca gcccgggtga cgcgcagtgg
     2521 ccggcggccg cgtacaacgg cgagtacatc gccgacatcg ccgccgactt ccgcgcgaag
     2581 aagacggtgc gcgccgacga ccgcgccttc actgcgtcgg gcgacgcggg cgacctcgac
     2641 ggcatccgcc agttcgccgt cgcctacctg cgccacgagc aggacctgga cctgcaggcc
     2701 ttcggcgtgc gcttcgacca ttacttcctc gaatcgagcc tgtattcgag cggccaggtc
     2761 gacgccaccg tgcagcgcct gatcgcctcc ggcaagacct tcgaggaagg cggggcgctg
     2821 tggctgcgca ccaccgacta cggcgacgac aaggaccgcg tgatgcgcaa atcggccccc
     2881 gccggggccg atgcgtcatc cgcgaacgcg agcgtctaca cgtacttcgt gcccgacgtg
     2941 gcgtatcacg tcaacaagtt cgagcgcggc ttcaccaagg ccatcaacat ccagggcggc
     3001 gaccaccacg gcacgatcgc gcgggtgcgc gccggcgtgc aggcggccgg tgcgggcgtg
     3061 cccgcgggct acccggaata cgtgctgcac aagatggtca cggtgatgaa gggcgggcag
     3121 gaggtgaaga tctccaagcg cgccggcagc tacgtgacct tgcgcgacct gatcgactgg
     3181 accagccgcg acgcggtgcg cttcttcctg ctcagccgca aggccgacag cgagttcacc
     3241 ttcgacgtcg acctcgcact gaagcgcaac gacgagaacc cggtgttcta cgtgcagtac
     3301 gcacatgcgc ggatctgctc ggtgatccgc aatttcgccg accagggcgg gcagcttgcc
     3361 ggcgcgcccg acctcgcgct gctgagcgcg ccgaccgagg ccgcgctgat gctcaggctt
     3421 gccgagttcc ccgcggtgct cgcgaacgcg gccacgagcc tggccccgca cgacctgacc
     3481 ttctacctgc gcgacctggc cggcgcgttc cacagctact acggggccga gcgcgtgctc
     3541 ggcgacgacg cggcgctgac gcgcgcacgc atcgcgctgc tggtcgcgac ccgtcaggtg
     3601 atccgcaacg ccctggcggt gctgggcgtc agtgcgcccg agcgcatgga ccgcgacgtc
     3661 gaacccaagc ccgagccggc atcggcctcc acaccatgac tagcacgcaa caacaacgtg
     3721 gcggtttcgc cgtcgggctg atcatcggcc tgctgatcgg cctggcgttg gcactgggcg
     3781 tggcgctgta cgtcaccaag gtgccgatcc cgttcgtcaa caaggtgccg caacgcactg
     3841 ccgaccagga cgcggccgag gtcgagaaga acaagaactg ggacccgaac agcccgctgt
     3901 ac
//
LOCUS       DATMJB010000660         9732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_340824, whole genome shotgun sequence.
ACCESSION   DATMJB010000660 DATMJB010000000
VERSION     DATMJB010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9732
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340824"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..858
                     /locus_tag="VNU48_13970"
     CDS             28..858
                     /locus_tag="VNU48_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HWI12435.1"
                     /translation="MSAIGGAAPAGASPVDGARHRHVFLAVLTALVALAWLALWAWVR
                     SPYGRYLDHADWTATGPAAFLCRVVPGGNVVVPALLYGAAWILMTAAMMLPTTLPLFN
                     AFDRLTARRADHARLLALLVLGYMAVWGAFGLLAHALHAALLSLVDSVPTLAWHGWLI
                     GAATIALAGAFQFSSLKHRCLEKCRTPLGFVIEHWRGQAQARHAFALGAQHGLFCVGC
                     CWALMLLMFALGTGSLGWMLLLAAVMAIEKNVRWGRRLSAPLGAALLAWAAVLVATHA
                     "
     gene            851..1738
                     /locus_tag="VNU48_13975"
     CDS             851..1738
                     /locus_tag="VNU48_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWI12436.1"
                     /translation="MHEGRAGLPFLQTLALTAAAMLAFAANSLLCRLALQRGLIDPAS
                     FASIRLVAGAVTLAVIVRLRSDRPAPGHADWLAATMLFAYVAFFSFAYLSLPAGTGAL
                     ILFGAVQVTMFSVGLRSGERFRPLAWFGLALAVAGLVYLVSPGVAAPAPLGAAMMATA
                     GVAWGVYSLRGRGVTDPLAATAGNFVRAAPLALVLSLLLAGNVHADAAGVALAIASGA
                     VTSGIGYVIWYAALSRLSAIRAATVQLSVPLIAAFGGVLLLSEAITPRLLAASVAILG
                     GIALVLATKSQGLRPPRPA"
     gene            1933..2862
                     /locus_tag="VNU48_13980"
     CDS             1933..2862
                     /locus_tag="VNU48_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HWI12437.1"
                     /translation="METKWLEDFVSLAETRSFSRSAQLRHVTQPAFSRRIQSLEAWAG
                     IDLVDRSSYPTRLTSAGETLHAQALEILGTLQASRNMMRSHKAASHDMVEFAVPHSLA
                     FTFFPHWVMDLRKRFGAMKSRLIALNVHDAVLHLTEGSCDLLLAYHHPSQPLQLNPDR
                     YEMLSLGHETLVAYAKGDASGQPLFSLPAPNGQAVPFLSYAPGAYLGRLVEVIAKLAP
                     EPLQLEPIYETDMAEGLKAMAIEGHGLAFLPASSVKKELKSKRLVRAAAPGAHELTMD
                     IRLYRERPEVARHAKPGAQALWAFLAASGDPHK"
     gene            2954..4507
                     /locus_tag="VNU48_13985"
     CDS             2954..4507
                     /locus_tag="VNU48_13985"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWI12438.1"
                     /translation="MLKLNDPDLFRQQVYLAGEWVDADSGATVAVTNPASGATLGTVP
                     MCGAAETARAIAAADQAQRSWRDVSAKERAATLRRLNDLMLANTDDLALIMTSEQGKP
                     LAEAKGEIAYAASFIEWFADEARRVYGDTIPATSGDKRIVAIKQPIGTTAAITPWNFP
                     TAMLTRKAGPALAAGCSMVVKPATQTPFSALAFAVLAERAGVPKGLFAVLTGAASQIG
                     GEMTRNPLVRKLSFTGSTEVGRTLMRQSADTIKKLSLELGGNAPFIVFDDADLDAAVE
                     GAMISKYRNTGQTCVCANRLYVQRGVLQAFTDKLVAKVKALKVGDGTEPGVNQGPLID
                     AKAVAKIEEHIADARAKGAEVLTGGKRHARGGLFFEPTVVGGATADMLFAKEETFGPL
                     APIFAFDTEAQAIAQANDTEFGLAAYFYSRDIGRVMRVAEQLESGIVGINTGLISTAE
                     APFGGVKQSGLGREGSKYGLDDYLEIKYLCFGGIDAPGARSALPLEGAPPSARQGRIA
                     ASARQETSR"
     gene            4504..5931
                     /locus_tag="VNU48_13990"
     CDS             4504..5931
                     /locus_tag="VNU48_13990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HWI12439.1"
                     /translation="MNPATTRREHDFLGEMEIPAEAYWGVHSARAVDNFPISGQTVAT
                     MPELVRALAFVKKAAAQTNGELGVIDPAQAAAIARACDDLIGGALHEQFVVDVIQGGA
                     GTSTNMNANEVIANRALEHMGFEKGRYDKLHPNDHVNASQSTNDVYPTALRVAAWFGI
                     EALLAAMADLRGAFEAKALEFKSVLKIGRTQLQDAVPMTLGQEFLAFAIMIGEDEQRL
                     REARALITEINLGATAIGTGINAPAGYGERVIPLLASVSGVPLVKAENLVEATQDTGS
                     FVQLSGVLKRVACKLSKVCNDLRLLSSGPQAGFGDIKLPARQAGSSIMPGKVNPVIPE
                     VMNQVAFEVIGNDVTITMASEAGQLQLNAFEPIMGYAIHKSVRHLTQACKTLQINCVE
                     GIQANHALLAKRVAESVTLVTALNPIIGYEKAALIAKTALAMQGKEGGTIAEVAEQLG
                     IMSQAEMRALLVPERLTQPVRLVAG"
     gene            6089..6982
                     /locus_tag="VNU48_13995"
     CDS             6089..6982
                     /locus_tag="VNU48_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HWI12440.1"
                     /translation="MKKLALALAAVLACGAATVATAADTLAKIKSAGKITIGTRDSSA
                     PLAYTTGDGKYVGYHVDVCLKIAEAIKANLKAPTMPIDYTVVTSANRVPLVQNGTVDM
                     ECGSTTNNAARQAQVAFAPTTFVTNVRTAVKASSGITSIEQLNGKTVATTTGTTSVQL
                     LRKRAGKIDFKELFGKDHADSFLLLESGRADAFVMDDNILAGLIVNSKAPAEFKIVGE
                     SLSEEPIAIMIPKDDPAYKKLVDDTVIGLMKSGELDKIYTKWFMAPIPPRSATVGMAM
                     SASLKNAIANPNDKPAEDYKK"
     gene            7154..7861
                     /locus_tag="VNU48_14000"
     CDS             7154..7861
                     /locus_tag="VNU48_14000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWI12441.1"
                     /translation="MGNWKWQVFLQDTGGGQTYLQWLMSAWGWTMSVALLALLIALVV
                     GSMIGILRTVPSKALSMFGEAWTELFRNVPLLVQLFVWYHVLPAIFLSLRSVPSFLLV
                     VFGLGLFTSARIAEQVKAGIQTLPKGQRYAGLAMGLTLPQTYRYVLMPVAFRVVIPPL
                     TSESMNIIKNSSVAFAVSIAELTFFAQQAAEETSRGVEVYLAVSFLYFISAFAINRVA
                     MVIEHRVQVPGMVGAGK"
     gene            7896..8564
                     /locus_tag="VNU48_14005"
     CDS             7896..8564
                     /locus_tag="VNU48_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWI12442.1"
                     /translation="MTALDFGFLNWNLVTNFIWKGLVFSVQLTLIAMVGGIIIGTLLA
                     LMRLSGKKWLVMAAAFYVNTLRSVPLVMVILWFFLLIPLLIGRSLGAELSAIITFTVF
                     EAAYYSEIMRAGIQSVSKGQVNAGYAVGMTYKQCMQLIVLPQAFRNMLPVLLTQTIIL
                     FQDTSLVYAIGAYDLLKGFEVAGKNFNRPVEAYLFAAVVYFVICFSLSMLVRRLQKKI
                     AIIR"
     gene            8576..9310
                     /locus_tag="VNU48_14010"
     CDS             8576..9310
                     /locus_tag="VNU48_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWI12443.1"
                     /translation="MIDIKNVSKWYSTFQVLTDCTTTIKKGEVVVVCGPSGSGKSTLI
                     KTVNALEPFQKGDIVVDGISIADPKTDLPKLRARVGMVFQHFELFPHLSVTENLTLAQ
                     IKVLGRSPDDAKARGLKMLDRVGLIAHKDKFPGQLSGGQQQRVAIARALSMDPIVMLF
                     DEPTSALDPEMVGEVLDVMVLLAKEGMTMMVVTHEMGFAKKVAHRVIFMDAGKIVEDC
                     TRDEFFGSPDARSPRAKEFLSKILQH"
     gene            9373..>9732
                     /locus_tag="VNU48_14015"
     CDS             9373..>9732
                     /locus_tag="VNU48_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWI12444.1"
                     /translation="MTDEITITRPDDWHLHVRDGAALAAVVPDSARQFGRAIIMPNLR
                     PPVTTTAQAVAYRQRLLAARPAGSDFEPLMTLYLTDNLPPEEIARAKDAGIVAVKLYP
                     AGATTNSDAGVTDLRKTY"
BASE COUNT         1528 a         3333 c         3327 g         1544 t
ORIGIN      
        1 cgcagtgcgg agcacggagg ttgcctgatg agcgctattg gcggcgctgc accggcaggc
       61 gcgtcgccgg tcgacggcgc gcgccatcgt cacgtgttcc tcgcggtgct gaccgcgctg
      121 gttgcgctcg cgtggctggc gctgtgggcg tgggtgcgca gcccgtacgg gcgctacctc
      181 gaccacgccg actggacggc gaccgggccg gccgcgttcc tgtgccgtgt cgttcccggc
      241 gggaacgtgg tcgtgcccgc gctgctctac ggcgccgcgt ggatcctgat gaccgcggcg
      301 atgatgctgc cgaccacctt gcccttgttc aatgccttcg atcggttgac ggcccggcgc
      361 gcggaccacg cgcgcctcct cgcactgctg gtcctcggct acatggcggt gtggggcgcc
      421 ttcgggctgc tggcgcatgc acttcacgcg gcgctgctgt cgctggtcga cagcgtgccg
      481 acgctggcct ggcatggctg gctgatcggt gccgcgacca tcgcgttggc cggggccttc
      541 cagttcagca gcctgaagca ccggtgcctg gaaaagtgcc gcacgccgct cggcttcgtc
      601 atcgagcact ggcgcgggca ggcacaggcg cgacacgcgt tcgcgctcgg cgcgcagcac
      661 ggcctgttct gcgtcgggtg ctgctgggcg ctgatgctgt tgatgttcgc gctcggcacc
      721 ggcagcctcg gctggatgct cttgctcgcc gcggtgatgg cgatcgaaaa gaatgtccgc
      781 tggggccgtc gtctcagcgc gccgctgggt gccgctctgc tggcctgggc ggcggttctg
      841 gtggccaccc atgcatgagg ggcgcgccgg cctgcccttc ctgcagaccc tggccctcac
      901 cgcggcggcg atgctcgcct tcgcggccaa ttcgctgctg tgccggctcg cgctgcaacg
      961 cgggctcatc gatccggcca gctttgccag catccggctg gtcgccgggg cggtcacgct
     1021 cgccgtcatc gtgcggctca gatcggaccg gcccgcgcct ggtcatgccg actggctcgc
     1081 cgcgaccatg ctgttcgcgt atgtcgcgtt cttctccttc gcctacctca gcttgcccgc
     1141 ggggacgggt gccttgatcc tgttcggcgc ggttcaggtg acgatgttca gcgtgggttt
     1201 gcgctcgggc gagaggttca gaccgcttgc ctggttcggt cttgccctgg ccgtcgcggg
     1261 gctggtctat ctggtgtcgc cgggtgtggc cgcaccggcg cccctcgggg cggccatgat
     1321 ggcgacggcc ggtgtggcgt ggggcgtgta ttcgctgcgc ggccgcggcg tgaccgaccc
     1381 gctggccgcc acggcgggca acttcgtgcg cgccgcgccg ctcgcgctgg tgctgagcct
     1441 gctgctcgcc ggcaacgtgc acgccgatgc cgccggggtc gccctggcca tcgcttccgg
     1501 cgcggtcacg tccggcatcg gctatgtgat ctggtacgcc gcgctgagcc ggctttccgc
     1561 gatccgcgcc gccaccgtgc agttgtcggt gccgctgatc gccgcattcg gcggggtgct
     1621 gctgctgtcg gaggcgatca caccacgcct gctggccgct tccgtggcga tcctcggggg
     1681 cattgcgctg gtgctggcca ccaagtcgca gggcttgcgc ccgccgcgtc ccgcgtgaac
     1741 tcggcgcacg gcaccttgac ccgctgaaac cgcgcctgca cctggccagc gacgaatcgt
     1801 cgttcgtcaa cggcggccac gcgcaccttg ttcgaatgag cgaacccggg gcgctgattc
     1861 ccccggggag aacttcggcg ccgggtcgcg caccgacgcg cgcgcggacc gcccctcgct
     1921 acactgcccg gcgtggaaac caagtggctc gaagacttcg tcagcctggc cgagacgcgc
     1981 agcttctcgc gctcggcgca gctgcgccat gtcacgcagc cggcgttctc gcgccgcatc
     2041 cagtcgctcg aggcctgggc cggcatcgac ctggtcgacc gctcctcgta cccgacccgg
     2101 ctcacgtcgg ccggcgagac cctgcacgcc caggcgctgg agatccttgg cacgctgcag
     2161 gcctcgcgca acatgatgcg cagccacaag gcggccagcc acgacatggt cgaattcgcg
     2221 gtgccgcatt cgctcgcgtt cacgttcttc ccgcactggg tgatggacct gcgcaagcgc
     2281 ttcggcgcga tgaagagccg gctgatcgcg ctcaacgtgc acgacgcggt gctgcacctg
     2341 accgagggca gctgcgacct gctgctcgcc tatcaccacc cgtcgcagcc gctgcagctc
     2401 aaccccgacc gctacgagat gctcagcctc gggcacgaga cgctggtcgc ctacgcgaag
     2461 ggcgacgcga gcgggcagcc gctgttcagc ctgcccgcgc cgaacgggca ggcggtgccg
     2521 ttcctgagtt acgcgccggg cgcctacctg ggccgcctgg tcgaggtgat cgccaagctc
     2581 gcgcccgagc cgctgcagct cgagccgatc tacgaaaccg acatggccga gggcctgaag
     2641 gcgatggcga tcgaaggcca tggcctggcc ttcctgccgg ccagctcggt caagaaggag
     2701 ctcaagagca agcgcctggt gcgcgccgca gcgcccggcg cacatgagct gacgatggac
     2761 atccgtctgt accgtgagcg ccccgaggtg gcgcgccacg ccaagcccgg cgcgcaggcg
     2821 ctgtgggcgt tcctggcggc ctcgggcgac cctcacaagt agccgcacag cacgcgtgtc
     2881 gaggagtcgg tgttcgagtc aggaatggct attcttgcca aggccctcga accctcctga
     2941 atcggagaac cgcatgctca agctcaatga tcccgacctg ttcaggcaac aggtctacct
     3001 cgccggcgaa tgggtcgatg ccgacagcgg cgcgaccgtg gccgtcacga acccggccag
     3061 cggcgcgacg ctcggcacgg tgccgatgtg cggcgccgcc gagaccgcgc gcgcgatcgc
     3121 ggccgccgac caggcccagc gttcctggcg cgacgtgagc gccaaggagc gcgccgcgac
     3181 gctgcgccgg ctcaacgacc tgatgctggc gaacaccgac gacctggcgc tgatcatgac
     3241 cagcgagcag ggcaagccgc tggccgaggc caagggcgag atcgcctacg ccgcgtcgtt
     3301 catcgagtgg ttcgccgacg aggcgcggcg cgtctacggc gacacgattc ccgcgacctc
     3361 gggcgacaag cgcatcgtcg cgatcaagca gccgatcggc acgaccgcgg cgatcacgcc
     3421 gtggaacttc ccgaccgcga tgctcacgcg caaggccggc ccggcgctgg ccgcgggctg
     3481 ctcgatggtc gtcaagccgg cgacgcagac gccgttctcg gcgctcgcgt tcgcggtgct
     3541 ggccgagcgc gccggtgtgc ccaaggggct gttcgcagtg ctgaccggcg cggccagcca
     3601 gatcggcggc gagatgacgc gcaacccgct ggtgcgcaag ctcagcttca ccggctcgac
     3661 cgaggtcggc cgcacgctga tgcgccagag cgccgacacg atcaagaagc tctcgctcga
     3721 actcggcggc aacgcgccgt tcatcgtctt cgacgacgcc gacctcgacg ccgcggtcga
     3781 gggtgcgatg atctcgaagt accgcaacac cggccagacc tgcgtctgcg caaaccgcct
     3841 gtacgtgcag cgcggcgtgt tgcaggcctt caccgacaag ctggtcgcga aggtcaaggc
     3901 gctgaaggtc ggcgacggca ccgagcccgg cgtgaaccag ggcccgctga tcgatgcgaa
     3961 ggcggtcgcg aagatcgagg agcacatcgc cgatgcgcgc gccaagggcg ccgaggtgct
     4021 gaccggcggc aagcgtcatg cgcgcggcgg cctgttcttc gagccgaccg tggtgggggg
     4081 tgcgaccgcc gacatgctgt tcgcgaagga agagaccttc ggcccgctcg cgccgatctt
     4141 cgcgttcgac accgaggcgc aggcgatcgc gcaggccaac gacaccgagt tcggcctcgc
     4201 ggcctacttc tacagccgcg acatcggccg cgtgatgcgc gtggccgagc aactcgagtc
     4261 gggcatcgtg ggcatcaaca ccgggctgat ctcgaccgcc gaggcgccgt tcggcggcgt
     4321 caagcagtcg ggcctgggcc gcgagggctc gaagtacggc ctggacgact acctcgagat
     4381 caagtacctg tgcttcggcg ggatcgacgc ccccggcgcg cgttcggcgc tccccctcga
     4441 gggggcgccg ccatcggccc ggcaaggccg gatcgcggcg tccgctcgac aggagacatc
     4501 ccgatgaacc cggcaacgac ccgccgcgag cacgacttcc tcggcgagat ggagattccc
     4561 gccgaggcct actggggcgt gcacagcgcg cgcgcggtcg acaacttccc gatcagcggg
     4621 cagacggtgg cgacgatgcc cgagctggtg cgcgcgctcg cgttcgtcaa gaaggcggcc
     4681 gcgcagacca atggcgagct cggcgtgatc gatccggcgc aggccgcggc gatcgcgcgc
     4741 gcctgcgacg acctgatcgg cggcgcgctg cacgagcagt tcgtcgtcga cgtgatccag
     4801 ggtggcgccg gcacctcgac caacatgaac gcgaacgagg tgatcgcgaa ccgcgcgctc
     4861 gagcacatgg gcttcgagaa gggccgctac gacaagctgc acccgaacga ccatgtcaac
     4921 gcgtcgcaga gcacgaacga cgtctacccg accgcgctgc gcgtggcggc gtggttcggc
     4981 atcgaggcgc tgctggcggc gatggccgac ctgcgcggcg cattcgaggc caaggcgctc
     5041 gagttcaaga gcgtgctgaa gatcggccgc acgcagctgc aggacgcggt gccgatgacg
     5101 ctgggccagg agttcctcgc gttcgcgatc atgatcggcg aggacgaaca gcgcctgcgc
     5161 gaggcgcgcg cgctgatcac cgagatcaac ctcggcgcga ccgcgatcgg caccggcatc
     5221 aacgcgccgg ccggctacgg cgagcgggtg atcccgctgc tcgccagcgt cagcggcgtg
     5281 ccgctcgtga aggccgagaa cctcgtcgag gcgacgcagg acaccggctc gttcgtgcag
     5341 ctctcggggg tgctcaagcg cgtcgcctgc aagctcagca aggtctgcaa cgacctgcgg
     5401 ctgctgtcca gcgggccgca ggccggcttc ggcgacatca agctgccggc gcgccaggcc
     5461 ggctcgtcga tcatgccggg caaggtgaac ccggtgatcc ccgaggtgat gaaccaggtc
     5521 gcgttcgagg tcatcggcaa cgacgtgacg atcacgatgg cctccgaagc cgggcagctg
     5581 caactcaacg ccttcgagcc gatcatgggt tacgcgatcc acaagagcgt gaggcacctg
     5641 acccaggcct gcaagacctt gcagatcaac tgcgtcgaag gcatccaggc caaccacgcg
     5701 ctgctggcca agcgcgtcgc cgagtcggtg acgctggtga ccgcgctgaa cccgatcatc
     5761 ggctacgaga aggcggcgct gattgcgaag accgcgctgg cgatgcaggg caaagaaggc
     5821 ggcacgatcg ccgaggtcgc cgagcagctg ggcatcatga gccaggccga gatgcgcgcg
     5881 ctgctcgtgc cggagcggct cacgcagccg gtgcggctgg tcgcgggttg agtcccgcgg
     5941 cgccccgctg cgacgcgcgc gcgcgggcac gaagcttgcg ccggctcagg caataccctc
     6001 gcattgcggg tgcctaccga ttccctgtcg gcgggcatcc gcaacaatcc gttcctcaat
     6061 tctgaaaccc ccacgaagga tgctccagat gaagaaactc gccctcgctc tcgccgccgt
     6121 gctggcatgc ggtgccgcca cggttgccac ggcggccgac acgctggcca agatcaagtc
     6181 cgccggcaag atcacgatcg gcacgcgcga ctcgtcggcc ccgctggcct acaccaccgg
     6241 cgacggcaag tacgtgggct accacgtcga tgtctgcctg aagatcgccg aggcgatcaa
     6301 ggcgaacctg aaggcaccga cgatgccgat cgactacacc gtcgtgacct cggcgaaccg
     6361 cgtgccgctg gtgcagaacg gcaccgtcga catggagtgc ggctcgacca cgaacaacgc
     6421 ggcgcgccag gcgcaggtcg cgttcgcccc gaccaccttc gtgaccaacg tgcgcaccgc
     6481 ggtgaaggcc agctcgggca tcacctcgat cgagcagctc aacggcaaga ccgtcgcgac
     6541 cacgaccggc acgacctcgg tgcagctgct gcgcaagcgc gccggcaaga tcgacttcaa
     6601 ggagctgttc ggcaaggacc acgccgactc gttcctgctg ctcgagtcgg gccgcgccga
     6661 tgccttcgtg atggacgaca acatcctggc cggcctgatc gtcaactcga aggcgccggc
     6721 cgagttcaag atcgtcggcg agtcgctgtc ggaagagccg atcgcgatca tgatcccgaa
     6781 ggacgacccg gcctacaaga agctggtcga cgacacggtg atcggcctga tgaagagcgg
     6841 cgagctcgac aagatctaca ccaagtggtt catggcgccg atcccgccgc gcagcgcgac
     6901 ggtgggcatg gcgatgtcgg cctcgctgaa gaacgcgatc gcgaacccga acgacaagcc
     6961 ggccgaagac tacaagaagt gatggcgcgc cgccggggcg acctcgggct ggcaccgacc
     7021 ggtccctgag gtaagcccta gcgtcggccg gcgcggagtt tgcttacatt gaccgagcgc
     7081 ggcgtggagc cttctgcgcc gcgcttcttc tttgtgccga tcgatcaaga acgagtacca
     7141 gcgggagcaa gccttgggta actggaaatg gcaggtgttc ctgcaggaca ccggcggcgg
     7201 gcagacctac ctgcaatggc tgatgtcggc ctggggctgg acgatgtcgg tggccttgct
     7261 ggcgctgctg atcgcgctgg tcgtggggtc gatgatcggc atcctgcgca ccgtgccgag
     7321 caaggcgctg tcgatgttcg gcgaggcctg gaccgagctg ttccgcaacg tgccgctgct
     7381 ggtgcagctg ttcgtctggt accacgtgct gccggcgatc ttcctgtcgc tgcgctcggt
     7441 gccgagcttc ctgctggtcg tgttcggcct gggcttgttc acctcggcgc gtatcgccga
     7501 gcaggtcaag gctggcatcc agacgctgcc caagggccag cgctacgccg gcctcgcgat
     7561 ggggctgacg ctgccgcaga cctaccgcta cgtgctgatg ccggtggcgt ttcgcgttgt
     7621 cattccgccg ctgaccagcg agtcgatgaa catcatcaag aactcgtcgg tcgcgttcgc
     7681 ggtgagcatc gccgagctga ccttcttcgc gcagcaggcc gccgaagaga cctcgcgcgg
     7741 cgtcgaggtc tacctcgcgg tctcgttcct gtacttcatc tccgcgttcg cgatcaaccg
     7801 cgtcgcgatg gtgatcgagc accgcgtcca ggtgcccggc atggtcggag caggcaaatg
     7861 atgcgccccc ttgagacggc tcggcgggag gtccaatgac tgccctggac ttcggtttcc
     7921 tgaactggaa cctcgtcacc aatttcatct ggaaagggct ggtcttctcg gttcagctga
     7981 cgctgatcgc gatggtcggc ggcatcatca tcggcacgct cctcgcgctg atgcggctgt
     8041 cgggcaagaa gtggctggtg atggcggcgg ccttctacgt caacacgctg cgctcggtcc
     8101 cgctggtgat ggtgatcctg tggttcttcc tgctgatccc gctgctgatc gggcgctcgc
     8161 tcggcgccga gctgtcggcg atcatcacgt tcacggtgtt cgaggcggcg tactactccg
     8221 agatcatgcg cgccggcatc cagtcggtct cgaagggcca ggtcaacgcc ggctacgcgg
     8281 tgggcatgac ctacaagcag tgcatgcagc tgatcgtgct gccgcaggcc tttcgcaaca
     8341 tgctgccggt gctgctgacg cagaccatca tcctgttcca ggacacgtcg ctcgtctacg
     8401 cgatcggtgc ctatgacctg ctgaagggct tcgaggtcgc gggcaagaac ttcaaccgcc
     8461 cggtcgaggc ctacctgttc gcggcggtcg tctacttcgt gatctgcttc agcctgtcga
     8521 tgctggtgcg ccggctgcag aagaagatcg ccatcattcg ctgaaggata gccccatgat
     8581 cgacatcaag aacgtctcca agtggtacag cactttccag gtgctgaccg actgcacgac
     8641 cacgatcaag aagggcgagg tcgtggtcgt ctgcggcccg tcgggctcgg gcaagtccac
     8701 gctgatcaag accgtgaacg cgctcgagcc gttccagaag ggcgacatcg tcgtcgacgg
     8761 catctcgatc gccgacccga agaccgatct gccgaagctg cgcgcgcgtg tcggcatggt
     8821 gttccagcac ttcgagctgt tcccgcacct gtcggtcacc gagaacctga cgctggcgca
     8881 gatcaaggtg ctgggccgca gccccgacga cgcgaaggcg cgcggcctga agatgctcga
     8941 ccgcgtcggc ctgatcgcgc acaaggacaa gttcccgggc cagctctcgg gcggccagca
     9001 gcagcgtgtc gcgatcgcgc gcgccctcag catggacccg atcgtgatgc tgttcgacga
     9061 gccgacctcg gcgctcgacc ccgagatggt cggcgaggtg ctcgacgtga tggtgctgct
     9121 ggccaaggag ggcatgacga tgatggtcgt cacgcacgag atgggcttcg cgaagaaggt
     9181 cgcgcaccgc gtgatcttca tggacgccgg caagatcgtc gaggactgca cgcgcgacga
     9241 gttcttcggc agccccgacg cgcgctcgcc gcgggcgaag gagttcctgt cgaagatcct
     9301 gcagcactag gacgcgaagc gggctcgatg cccgcctcgt gagtgcgcgc agcgggtgcg
     9361 agaatcaagc gcatgacgga cgagatcacg atcacccgcc ccgacgactg gcatctgcac
     9421 gtgcgcgacg gggccgcgct cgcggcggtc gtgcccgaca gcgcgcgcca gttcggccgc
     9481 gcgatcatca tgccgaacct gcgcccgccg gtgacgacga cggcccaggc cgtcgcctac
     9541 cgccagcgcc tgctcgccgc acgcccggcc ggcagcgact tcgagccgct gatgacgctg
     9601 tacctgaccg acaacctgcc gcccgaggag atcgcgcgcg ccaaggacgc cggcatcgtc
     9661 gccgtgaagc tctacccggc cggtgcgacg accaacagcg acgccggcgt caccgacctg
     9721 cgcaagacct ac
//
LOCUS       DATMJB010000661         3009 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_716887, whole genome shotgun sequence.
ACCESSION   DATMJB010000661 DATMJB010000000
VERSION     DATMJB010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716887"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="VNU48_14020"
     CDS             complement(<1..475)
                     /locus_tag="VNU48_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HWI12445.1"
                     /translation="MKDSLRQQFERLALRLSELDATLADPSVAGDMKRYRALSREHAE
                     VAGLVERFRRFEQRERDLADAQQMAADPEMADLAQQEITEARADVERLHLELQTALLP
                     RDPDDARNAFVEIRAGTGGDESALFAGDLARMYLRHCERRGWKSEVMSESLSDLGG"
     gene            complement(558..1859)
                     /gene="hemA"
                     /locus_tag="VNU48_14025"
     CDS             complement(558..1859)
                     /gene="hemA"
                     /locus_tag="VNU48_14025"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HWI12446.1"
                     /translation="MSVFALGLNHTTAPVDLRGRFAFAPEQLVPTLQAFRERLQRVRE
                     VAIVSTCNRTELYVGADASLVNAAGLVHPAVDWLAGVGGVPSHTLREHAYLLEGGAAA
                     RHAFRVASGLDSMVLGEPQILGQLKQAVREAETAGTLGGALHQMFQRSFAVAKEVRSS
                     TEIGAHSISMAAAAVRLASQLFEDLRATKVLFVGAGEMIELVATHFAARNPRAMAVAN
                     RTLERGEKLAGRFGAEALRLADLPARLAEFDIVISSTASSLPIIGLGAVERALKQRKH
                     RPMFMVDLAVPRDIEPEVARLSDVYLYTVDDLSTLVQRGSAQRQAAVEQAEAIIETGV
                     QSFVHWMGQRGSVPLIQALHSQADDWRAAEIARARKMLARGEDIDAVLEALSRGLTQK
                     MLHGTLAELHSADGDQRAQLAQTVSRLFLRQNAHPPVDEPR"
     gene            1928..2209
                     /locus_tag="VNU48_14030"
     CDS             1928..2209
                     /locus_tag="VNU48_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12447.1"
                     /translation="MGPIDAFWHLLNFFAPAVGVGLLAGGLAKLLWRRELKGVAWMRL
                     AGWAAAAGAAVLVAGLVVFGHDGKMATYAAMVTACALTLWWVGFGPLRR"
     gene            complement(2216..>3009)
                     /locus_tag="VNU48_14035"
     CDS             complement(2216..>3009)
                     /locus_tag="VNU48_14035"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HWI12448.1"
                     /translation="QGVKEVTLLGQNVNAYRGAMGGTADIADFALLIEYVAAIPGIER
                     IRYTTSHPNEFTQRLIDVYAKVPQLVNHLHLPLQHGSDRILMAMKRGYTAMEYKSTVR
                     KLRAVRPGISMSSDFIVGFPGETEADFAQTMKLIDDIGFDDSFSFVFSPRPGTPAANL
                     ADATPPAEKLERLHVLQKAITESARAISESRLGTVQRVLVEGPSRKDPRELSGRTECN
                     RMVSFQGGPNSARLIGRMVDVTITEVNSHSLRGELRQREPEALAV"
BASE COUNT          415 a         1035 c         1080 g          479 t
ORIGIN      
        1 agccgccgag atcgctgagg ctctcgctca tgacctcgga cttccagccc cggcgctcgc
       61 agtggcgcag gtacatgcgc gccagatcgc cggcgaacag cgccgattcg tcgccgccgg
      121 tgccggcacg gatctcgacg aaggcgttgc gcgcatcgtc cgggtcgcgc ggcagcagcg
      181 ccgtctgcag ctcgaggtgc aggcgctcga catcggcgcg cgcctcggtg atctcctgct
      241 gtgcaaggtc ggccatctcg gggtcggccg ccatctgctg ggcatccgcg aggtcccgct
      301 cgcgttgctc gaagcggcgg aagcgctcga ccaacccggc cacctcggca tgttcgcgcg
      361 acagcgcgcg gtagcgcttc atgtcgccgg cgacgctcgg gtcggcgagc gtcgcgtcga
      421 gctcggacag gcgcagcgcg aggcgttcga attgctgacg gagagagtct ttcatgcggg
      481 tgctcgaagt ggaccacgga gcagcacgca cgacacaacg cacgcgggct ccgctgaagg
      541 cggaggcgcg tgcgtcgcta acgcggttcg tcgaccggcg ggtgcgcgtt ctggcgcagg
      601 aacaggcgcg agaccgtctg cgcgagctgt gcgcgctggt cgccgtcggc cgagtgcagt
      661 tcggccagcg tgccgtgcag catcttctgc gtcaggccgc gcgacagcgc ttcgagcacc
      721 gcgtcgatgt cttcgccgcg cgccagcatc ttgcgggcac gcgcgatctc ggcggcgcgc
      781 cagtcgtcgg cctgcgaatg cagtgcctgg atcagcggca ccgaaccgcg ctggcccatc
      841 cagtggacga aactctgcac gccggtctcg atgatcgcct cggcctgctc gaccgcggcc
      901 tggcgctgtg cgctgccgcg ctgcaccagc gtcgacagat cgtcgaccgt gtagaggtag
      961 acgtccgaca gccgcgccac ctcgggctcg atgtcgcgag gcaccgcgag gtcgaccatg
     1021 aacatcggcc ggtgcttgcg ctgcttcagc gcgcgctcca ccgcgcccag gccgatgatc
     1081 ggcaggctgc tggcggtgct cgagatcacg atgtcgaact cggccaggcg ggccggcagg
     1141 tcggccaggc gcagcgcctc ggcgccgaag cggcctgcca gcttctcgcc gcgctcgagc
     1201 gtgcggttgg ccaccgccat cgcgcgcggg ttgcgcgcgg cgaagtgcgt cgcgaccagc
     1261 tcgatcattt cgccggcgcc gacgaacagc accttggtcg cgcgcaggtc ctcgaacaac
     1321 tgcgaggcca ggcgcaccgc ggcggcggcc atgctgatcg aatgcgcacc gatctcggtc
     1381 gagctgcgca cttccttggc gaccgcgaac gagcgctgga acatctggtg cagcgcgccg
     1441 cccagcgtgc cggcggtttc ggcctcgcgc acggcctgct tcagctggcc gagaatctgc
     1501 ggctcgccca gcaccatcga atcgagaccg ctcgcgacgc ggaacgcgtg gcgcgccgcg
     1561 gcgccgcctt cgagcaggta ggcgtgttcg cgcaaggtgt ggctcgggac gccgccgacg
     1621 ccggcgagcc agtcgaccgc cgggtgcacg agcccggcgg cgtttaccag gctggcgtcg
     1681 gcgccgacgt agagctcggt gcggttgcag gtcgagacga tcgcgacctc gcggacgcgc
     1741 tgcaggcgct cgcgaaacgc ctgcaaggtc ggcacgagct gctccggcgc gaacgcgaac
     1801 cggccgcgca gatcgaccgg cgcggtggtg tggttcagcc cgagggcgaa gacgctcatt
     1861 gccggattat aaaatcgccg ccgttcgcgc gcccttgcgc gccgtcaagc cacccgaagg
     1921 acaccgcgtg ggcccgatcg atgccttctg gcacctgctg aacttcttcg caccggcggt
     1981 cggcgtcggc ctgctggccg gcggcctggc caagctgctg tggcggcgcg aactgaaggg
     2041 cgtggcctgg atgcgcctgg ccggctgggc ggcggcggcc ggcgcggccg tgctggtcgc
     2101 gggcctggtc gtgtttgggc acgacggcaa gatggcgacc tatgcggcga tggtgacggc
     2161 gtgcgcgctg acgctgtggt gggtcggctt cggcccactg cgacgctgac accgttcaga
     2221 cggccagcgc ctcgggctcg cgctgcctca gctcgccgcg cagcgagtgc gaattcacct
     2281 cggtgatggt cacatcgacc atccggccga tcaggcgcgc cgaattcggc ccgccctgga
     2341 agctgaccat gcggttgcat tcggtgcggc cgctcagctc gcgcgggtcc ttgcgcgacg
     2401 ggccctcgac cagcacccgc tgcaccgtgc ccaggcgcga ctcgctgatc gcacgggcgc
     2461 tctcggtgat cgccttctgc agcacgtgca ggcgctcgag tttctcggcg ggcggcgtgg
     2521 catccgccag gttcgcggcc ggcgtgcccg ggcgcgggct gaagacgaag ctgaagctgt
     2581 cgtcgaagcc gatgtcgtcg atcagcttca tcgtctgcgc gaagtcggcc tcggtctcgc
     2641 cggggaagcc gacgatgaag tcgctcgaca tcgagatgcc cggccgcacc gcgcgcagct
     2701 tgcgcacggt gctcttgtat tccatcgcgg tgtagccgcg cttcatcgcc atcagaatgc
     2761 ggtcgctgcc atgctgcaac ggcagatgca ggtggttcac gagttgcggc accttcgcgt
     2821 agacgtcgat caggcgctgc gtgaattcgt tcgggtgact ggtcgtgtag cggatgcgtt
     2881 cgatgccggg gatcgccgcg acgtactcga tcagcagcgc gaagtcggcg atgtcggccg
     2941 tgccgcccat cgcgccgcgg taggcgttga cgttctggcc cagcagcgtc acttccttga
     3001 cgccctggt
//
LOCUS       DATMJB010000662         2335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_149661, whole genome shotgun sequence.
ACCESSION   DATMJB010000662 DATMJB010000000
VERSION     DATMJB010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149661"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VNU48_14040"
     CDS             complement(<1..480)
                     /locus_tag="VNU48_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HWI12449.1"
                     /translation="MPELILHHYASSPFSEKVRLILGFKGLAWRSVIVPNIMPKPDVV
                     ALTGGYRRTPFMQIGADIYCDTALMCRVIDALEPEPPLYPAATAGIADIVAQWADTTL
                     FWTAVPFTLQPAGVPHVLKDVPPEMLKAFGADRAAMNPNMKRASVADGAAALVTYLAR
                     "
     gene            complement(504..2204)
                     /locus_tag="VNU48_14045"
     CDS             complement(504..2204)
                     /locus_tag="VNU48_14045"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HWI12450.1"
                     /translation="MNSATHEVLNQPAPLVGHNLYDTDRALRDALRFHAPRRDEQQLA
                     TLGALAGDEAMQTHARLANIHKPALHTHDRFGRRSDTVEFHPSYHALLGAAVGAGLHA
                     TPWSQGLGAHIEREGPRGGAHIERAAGFILFTQLEPSVLCPVSMTYAVTPALRGNAAV
                     FADWFDKLASTRYDPGLRVVGAKAGVTMGMGMTEKQGGSDVRANTTRADYLEDTPWGR
                     CYRLVGHKWFFSAPMSDAFLVLAQTPAGLSCFFMPRVLPDGSLNGIAIQRLKDKLGNQ
                     ANASAEVEFNGALAWLVGDEGRGIPQILEMGTLTRLDCALGTAGLMRGATALALHHAS
                     QRSAFGKPLIDQPLMKNVLADLAIESEAATALALRLARAIDRADDPFEALMRRVLTPV
                     AKFWICKRGAALAQEAMECLGGNGYVEEHGEGVMARIYREMPLNSIWEGAGNIMALDV
                     LRAMKKRDGTGGDVVGALAAELDTARGANASLDRFADALIARFNDAADGGGDEAGARR
                     LAQDIALAVQGSLLRRHAPDGVFEAFCRSRLSGDGPQVFGTLPSAVDVDSIIQRAMPR
                     "
     gene            complement(2212..>2335)
                     /locus_tag="VNU48_14050"
     CDS             complement(2212..>2335)
                     /locus_tag="VNU48_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HWI12451.1"
                     /translation="VASGKLKYRESVAQGLAAAPEAFLGLLKGRNFGKQVVKLI"
BASE COUNT          346 a          806 c          811 g          372 t
ORIGIN      
        1 gcgagcgagg taggtgacca gtgcggcggc gccgtcggcg acgctcgcgc gcttcatgtt
       61 cgggttcatc gcggcacggt cggcgccgaa ggccttcagc atctccggcg gcacgtcttt
      121 cagcacatgc ggcacgccgg cgggttgcag cgtgaacggc acggcggtcc agaacagcgt
      181 cgtgtcggcc cactgcgcga cgatgtcggc gatgcccgcg gtggccgccg ggtacagcgg
      241 cggctcgggc tcgagcgcat cgatgacgcg acacatcagt gcggtgtcgc agtagatgtc
      301 ggcgccgatc tgcatgaacg gcgtgcggcg gtagccgccg gtcaacgcga ccacgtccgg
      361 cttgggcatg atgttcggca cgatcaccga acgccatgcc aggcccttga agccgaggat
      421 caggcgcacc ttttcagaga acggcgagct ggcgtagtgg tgcaggatga gttcgggcac
      481 ggtgactttc gggttcggcc gcgtcaacgg ggcatggcgc gctggatgat cgaatcgaca
      541 tcgacggcgc tcggcaaggt gccgaacacc tgcgggccgt cgccgctcag gcgcgagcgg
      601 cagaacgcct cgaacacgcc atcgggcgcg tggcggcgca gcagcgagcc ctgcaccgcg
      661 agcgcgatgt cttgcgcgag gcggcgcgca ccggcctcgt caccaccgcc gtcggcggca
      721 tcgttgaagc gcgcgatcag cgcatcggcg aagcggtcga gcgaggcgtt cgcaccgcgc
      781 gcggtgtcga gctcggcggc gagcgcgccg accacgtcgc cgcctgtgcc gtcgcgcttt
      841 ttcatcgcgc gcaacacgtc gagggccatg atgttgcctg ccccctccca gatcgagttc
      901 agcggcatct cgcggtagat gcgcgccatc acgccctcgc cgtgctcttc gacatagccg
      961 ttgccgccga ggcactccat cgcctcctgc gccagggcag caccgcgctt gcagatccaa
     1021 aacttcgcga ccggcgtcag cacgcggcgc atcagcgcct cgaacggatc gtctgcccga
     1081 tcgatcgcgc gcgccaggcg cagcgcgagt gcggtcgcgg cctcgctctc gatcgcgagg
     1141 tcggcgagca cgttcttcat cagcggctga tcgatcagcg gtttgccgaa cgcgctgcgc
     1201 tgcgacgcat ggtgcagcgc cagcgcggtc gcgccgcgca tcaggccggc cgtgccgagc
     1261 gcgcaatcga gccgcgtcaa cgtacccatt tcgagaatct gcgggatgcc gcgcccttcg
     1321 tcgccgacca gccacgcgag cgcgccgttg aactcgactt cggcgctcgc gttggcctgg
     1381 ttgccgagct tgtctttcaa gcgttgaatg gcgatcccgt tgagcgatcc atccggcagc
     1441 acgcgtggca tgaagaagca cgacaggccc gccggcgtct gcgccagcac gagaaacgca
     1501 tcgctcatcg gcgccgaaaa gaaccacttg tggccgacga gccggtagca gcggccccaa
     1561 ggcgtgtctt cgaggtagtc agcgcgcgtc gtgttcgcgc gcacatccga gccgccctgc
     1621 ttctcggtca tgcccatgcc catcgtcacg ccggccttcg cgccgaccac gcgcagcccc
     1681 gggtcgtagc gcgtgctcgc gagcttgtcg aaccagtcgg cgaacacggc ggcgttgccg
     1741 cgcagcgcgg gcgtcaccgc gtaggtcatc gagaccgggc acagcaccga gggttcgagc
     1801 tgcgtgaaca ggatgaagcc cgcggcgcgc tctatgtgcg cgccgcctcg tggcccttcg
     1861 cgctcgatgt gcgcgcccaa gccctgcgac cacggcgtcg cgtgcaggcc cgcgccgacc
     1921 gccgcgccca gcagtgcgtg gtagctcggg tggaactcga ccgtgtcgct gcggcggccg
     1981 aagcggtcgt gcgtgtgaag tgcgggcttg tggatgttgg ccaggcgtgc gtgcgtctgc
     2041 atcgcctcgt cgccggcgag cgcgccgagc gtcgcgagtt gctgttcgtc gcgccgtggc
     2101 gcgtgaaaac gcagcgcatc ccgcagcgcg cggtcggtgt cgtagaggtt gtgacccacc
     2161 agcggcgcgg gctggttcag cacctcatgc gtcgcgctgt tcatcgcggc ctcagatcag
     2221 cttgacaacc tgcttgccga agttcctgcc cttcagcagg ccgaggaagg cctccggtgc
     2281 ggcggccaga ccttgcgcaa ccgattcgcg gtacttcagc ttgccgctcg cgacc
//
LOCUS       DATMJB010000663         5751 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_827062, whole genome shotgun sequence.
ACCESSION   DATMJB010000663 DATMJB010000000
VERSION     DATMJB010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827062"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..455
                     /gene="clpS"
                     /locus_tag="VNU48_14055"
     CDS             99..455
                     /gene="clpS"
                     /locus_tag="VNU48_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HWI12452.1"
                     /translation="MPDSTPKPPAPPPVRTAPDDGSGAVVVERQAQKTEPPRMYQVVL
                     LNDDYTPMEFVVMVLQEYFKHDLETATQIMLKIHHDGRGVCGVYSKDVAATKVELVLA
                     AARRGGHPLQCIMEAA"
     gene            452..2758
                     /gene="clpA"
                     /locus_tag="VNU48_14060"
     CDS             452..2758
                     /gene="clpA"
                     /locus_tag="VNU48_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HWI12453.1"
                     /translation="MIAQELEVSLHMAFVEARQQRHEFITVEHLLMALLDNPSAAEVL
                     RACSANADDLRKSLLGFIKENTPTVGGSDEVDTQPTLGFQRVIQRAIMHVQSTGSGKK
                     EVTGANVLVAIFGEKDSHAVYYLHQQGVTRLDVVNFIAHGIRKSDPPEPAKPGESGSS
                     GNEGEKEEGDGKGSPLDQFTQNLNQLARDGKIDPLIGRELEVERVIQILCRRRKNNPL
                     LVGEAGVGKTAIAEGLAWRITQNDVPEVLAGSMVYSLDMGALLAGTKYRGDFEQRLKG
                     VLKQLKDQPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSSGAMKCIGATTFTEY
                     RGIFEKDAALSRRFQKVDVTEPSIEQTVEILKGLKSRFEEHHSVKYAVGALQAAAELS
                     AKFINDRHLPDKAIDVIDEAGAAQRILPKSKQKKTITRAEVEDIVAKIARIPPASVSS
                     DDRGKLKTLDRDLNSVVFGQEPAIEALASAIKMARSGLGRPDKPIGSFLFSGPTGVGK
                     TEVAKQLAYILGIELIRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAISKKPHA
                     VLLLDEIEKAHPDVFNVLLQVMDHGTLTDNNGRKADFRSVIIIMTTNAGAETMNKATI
                     GFTNAREQGDEMADIKRLFTPEFRNRLDATVSFKALDEEIILRVVDKFLLQLESQLGE
                     KKVEVTFTDALRKQLAKKGFDPLMGARPMQRLIQDTIRRALADELLFGRLVDGGRLTV
                     DVDADGKPVLDIQPTKKIDKPKAEPATA"
     gene            complement(2811..3581)
                     /locus_tag="VNU48_14065"
     CDS             complement(2811..3581)
                     /locus_tag="VNU48_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HWI12454.1"
                     /translation="MRAWLQRWHERRTLARRAIPDALWALTLARFHFLGWRDAADIAR
                     LRDMATLFLADKEFSGAHGLRVDDEMAVAIAAQACLPVLELGLAWYDGFKGVVVHADA
                     VLAQREFVDDAGVVHEYEEELSGEAMHGGPVMLAWRDVADAGESAGEGYNVVVHEFVH
                     VLDMRDGSADGVPPLPDRAARAAWLAVLTAEYDTFCERVDADEDTLLDPYGAQAPEEF
                     FAVASEAFFVAPRDFRAEHPRLYELLRGFFKQDPAAHC"
     gene            complement(3584..4378)
                     /locus_tag="VNU48_14070"
     CDS             complement(3584..4378)
                     /locus_tag="VNU48_14070"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HWI12455.1"
                     /translation="MNVAPPALREIDAPPAWRRIDFISDLHLAEDTPRTFAAWRDYLL
                     HTRADAVFILGDLFEAWVGDDARHEGFEAEGAAVLKAAATRRPMFFMVGNRDFLLGDA
                     MLSACGVEGLADPTLLAAFGARVLLSHGDAWCIADVEYQQFRRQVRDPAWQAQVLARP
                     LAGRRALARNLRSESERKASEHRGDWVDVDTPTALQWLERTRSSTLVHGHTHRPATQS
                     LAPGLERHVLSDWDLDHGGTPRAEVLRWERGGWSRLAPADAVGPVG"
     gene            complement(4396..4896)
                     /locus_tag="VNU48_14075"
     CDS             complement(4396..4896)
                     /locus_tag="VNU48_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWI12456.1"
                     /translation="MTQVKLTTNHGDIVLQLNAEKAPLTVANFIEYVKAGHYENTVFH
                     RVIGNFMIQGGGFEPGMRQKATRATITNEANNGLKNNRYTLAMARTSAPHSASSQFFI
                     NATDNNFLNFTAETASGWGYAVFAKVVAGSEVVDAIERVKTGSKGGHGDVPLDDVVIL
                     KASRVA"
     gene            5146..>5751
                     /locus_tag="VNU48_14080"
     CDS             5146..>5751
                     /locus_tag="VNU48_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003077604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HWI12457.1"
                     /translation="MSKPTVDPTSNSKTGPAVPVNFLRPIIQADLDSGKHTQIVTRFP
                     PEPNGYLHIGHAKSICVNFGLAQEFGGVTHLRFDDTNPAKEDQEYIDAIESDVKWLGF
                     EWSGEVRYASQYFDQLHDWAVELIKSGKAYVDDLTPEQAKQYRGSLTEPGKNSPFRDR
                     SVEENLDLFARMKAGEFPDGARVLRAKIDMASPNMNLRDPIM"
BASE COUNT         1045 a         1905 c         1910 g          891 t
ORIGIN      
        1 cgtgcacgtt gcttatttcc ggtcttgtgg ttccacgccc agtcgcaata tgggaacgga
       61 tgaaacgctt gcgcggcaac tcgatagaat gaattcccat gcctgacagc acgccgaaac
      121 cacccgcgcc accgccggtg cggactgcgc ccgacgacgg gagcggggcg gttgttgtcg
      181 agcgccaggc acagaagacc gaaccgccgc gcatgtacca ggtggtgctg ctcaacgacg
      241 actacacccc gatggagttc gtcgtgatgg tgcttcagga atacttcaaa cacgacctcg
      301 aaaccgcgac ccagatcatg ctgaagatcc accacgacgg gcgcggcgtc tgcggcgtct
      361 actcgaagga cgtcgccgca accaaggtcg aattggtgct ggctgccgcg cgccgcggtg
      421 gtcacccgtt gcaatgcatt atggaggccg catgattgcc caggaacttg aagtcagcct
      481 gcacatggcg ttcgtcgagg cgcgccagca gcgccacgag ttcatcaccg tcgagcacct
      541 gttgatggcg ctgctcgaca acccgtcggc ggccgaggtg ctgcgcgcct gctcggccaa
      601 cgccgacgac ctgcgcaaga gcctgctcgg cttcatcaag gagaacacgc ccaccgtggg
      661 cggctccgac gaggtcgaca cgcagcccac gctgggcttc cagcgcgtga tccagcgcgc
      721 gatcatgcac gtgcagtcca ccggcagcgg caagaaggaa gtgaccggcg cgaatgtgct
      781 ggtcgcgatc ttcggcgaga aggactcgca cgcggtgtac tacctgcacc agcagggcgt
      841 gacgcgcctg gacgtggtca atttcatcgc ccacggcatc cgcaagagcg acccgcccga
      901 gccggccaag cccggcgaga gtggctccag cggcaacgag ggcgagaagg aagagggcga
      961 cggcaaaggc tcgccgctgg accagttcac gcagaacctg aaccagctcg cccgcgacgg
     1021 caagatcgac ccgctgatcg gccgcgagct cgaggtcgag cgcgtgatcc agatcctgtg
     1081 ccggcggcgc aagaacaacc cactgctggt cggcgaggcc ggcgtgggca agaccgcgat
     1141 cgccgagggc ctggcctggc gcatcacgca gaacgacgtg cccgaggtgc tggccggctc
     1201 gatggtgtac tcgctcgaca tgggcgcgtt gctggccggc accaagtacc gcggcgactt
     1261 cgagcagcgc ctgaaaggcg tgctcaagca gctgaaggac cagccgaacg cgatcctgtt
     1321 catcgacgag atccacacct tgatcggcgc cggcgcggcc tcggggggca cgctggacgc
     1381 gagcaacctg ctcaagccgg cgctgagctc cggcgcgatg aagtgcatcg gcgcgaccac
     1441 cttcaccgag tatcgcggca tcttcgagaa agacgccgcg ctgtcgcggc gcttccagaa
     1501 ggtcgatgtg accgagccgt cgatcgagca gacggtcgag atcctgaagg ggctgaagtc
     1561 gcgcttcgag gagcaccaca gcgtcaagta cgcggtcggt gcgctgcaag ccgcggccga
     1621 gctgtcggcc aagttcatca acgaccggca cctgcccgac aaggcgatcg acgtgatcga
     1681 cgaggccggt gcggcgcagc gcatcctgcc caagagcaag cagaagaaga ccatcacgcg
     1741 cgccgaggtc gaggacatcg tcgcgaagat cgcgcgcatc ccgcccgcca gcgtgtcgag
     1801 cgacgaccgc ggcaagctca agacgctgga ccgcgacctg aacagtgtgg tgttcggcca
     1861 ggagccggcg atcgaggcgc tcgcgtcggc gatcaagatg gcgcgctccg gcctgggccg
     1921 cccggacaag ccgatcggct cgttcctgtt cagcggcccg accggcgtcg gcaagaccga
     1981 ggtcgcgaag cagctcgcct acatcctggg catcgaactg atccgcttcg acatgagcga
     2041 gtacatggaa cgccacgcgg tcagccgcct gatcggcgcg ccgccgggct atgtcggctt
     2101 cgatcagggc ggtctgctga ccgaggcgat ctcgaagaag ccgcacgcgg tgctgctgct
     2161 cgacgagatc gagaaggcgc acccggacgt cttcaacgtg ctgctgcagg tcatggacca
     2221 cggcaccttg accgacaaca acgggcgcaa ggccgacttc cgcagcgtca tcatcatcat
     2281 gacgacgaac gccggcgccg aaacgatgaa caaggcgacg atcggcttca cgaacgcgcg
     2341 cgagcagggc gacgagatgg ccgacatcaa gcggctgttc acgcccgagt tccgcaatcg
     2401 actggatgcg acggtcagct tcaaggcctt ggacgaagag atcatcctgc gcgtggtcga
     2461 caagttcctg ctgcagctcg agagccagct cggcgagaag aaggtcgagg tcacgttcac
     2521 cgacgcgctg cgcaagcagc tcgcgaagaa gggcttcgac ccgctgatgg gcgcgcggcc
     2581 gatgcagcgc ctgattcagg acacgatccg gcgcgcgctg gccgacgagt tgctgttcgg
     2641 gcgcctggtc gacggcggcc ggctgacggt cgatgtcgac gccgacggca agccggtgct
     2701 cgacatccag ccgaccaaga agatcgacaa gccgaaggcc gagcccgcca cggcgtagac
     2761 gatcgaggtc tggcaagggg gagccgcgag gctccctttg tctttgccgg tcagcagtgc
     2821 gcggccgggt cctgcttgaa gaagccgcgc agcagctcgt acaggcgcgg gtgctcggcg
     2881 cggaagtcgc gcggtgcgac gaagaaggcc tcggaggcga cggcgaagaa ctcttccggc
     2941 gcctgcgcgc cgtacggatc cagcagcgtg tcttcgtccg cgtcgacacg ctcgcagaac
     3001 gtgtcgtact cggccgtcag cacggcgagc caggccgcgc gcgccgcgcg atcgggcagc
     3061 ggcggcacgc cgtcggcgct gccatcgcgc atgtccagca catgcacgaa ctcgtgcacg
     3121 acgacgttgt agccttcgcc ggccgattcg cccgcgtcgg ccacatcgcg ccaggccagc
     3181 atcacggggc cgccgtgcat cgcctcgccg gagagctctt cctcgtattc gtggaccacg
     3241 ccggcgtcgt cgacgaactc gcgctgggcg agcaccgcat ccgcgtgcac gacgacgccc
     3301 ttgaaaccgt cgtaccaggc caggccgagt tcgagcaccg gcaggcacgc ctgcgctgcg
     3361 atcgccaccg ccatttcgtc gtcgacgcgc aagccgtgcg cgccgctgaa ctccttgtcg
     3421 gcgaggaaca gcgtggccat gtcgcgcagc cgggcaatgt cggccgcgtc acgccaaccc
     3481 aggaaatgaa agcgcgccag cgtcagcgcc cacagtgcat cggggatcgc gcgccgcgcc
     3541 agggtgcgcc tctcgtgcca gcgctgcagc caggcgcgca ccgtcagccg accggcccga
     3601 ccgcgtcggc cggcgccagc cgcgaccagc cgccgcgctc ccaacgcagc acttcggcgc
     3661 gcggcgtgcc gccgtggtcc agatcccagt cgctcagcac gtggcgttcg aggcccggtg
     3721 ccaacgactg cgtcgcgggc cgatgcgtgt ggccgtgcac gagagtgctt gaacgcgtgc
     3781 gctccagcca ttgcagcgcg gtcggcgtgt cgacgtcgac ccagtcgccc cgatgctcgg
     3841 atgccttgcg ctcgctttcg gagcgcaggt tgcgcgccag cgcgcgccgc ccggccagcg
     3901 gccgcgcgag cacctgggcc tgccaggcgg ggtcgcgcac ctggcggcgg aactgctggt
     3961 actcgacatc ggcgatgcac caggcgtcgc cgtgcgacag cagcacgcgc gcgccgaagg
     4021 ccgcaagcag cgtcgggtcg gccaggcctt cgacaccgca ggccgagagc atcgcgtcgc
     4081 cgagcaggaa atcgcggttg ccaaccatga agaacatggg ccgccgcgtc gcggccgctt
     4141 tcagcacggc cgcgccctcg gcttcgaatc cttcgtgccg tgcgtcgtcg ccgacccacg
     4201 cttcgaacag gtcgccgagg atgaagaccg catcggcgcg cgtgtgaagc aggtagtcgc
     4261 gccacgcggc gaaggtgcgt ggtgtgtcct cggccaggtg caggtcgctg atgaaatcga
     4321 tgcggcgcca cgccggcggc gcatcgatct cgcgcagcgc gggcggggcg acgttcacgc
     4381 gacgagccgc ggggctcagg cgacccgcga cgccttcagg atcacgacat cgtccagcgg
     4441 cacgtcgccg tggccgccct tgctgccggt cttgacgcgc tcgatcgcat cgacgacttc
     4501 gctgcccgcg accaccttgg cgaacaccgc gtagccccag cccgacgcgg tctcggcggt
     4561 gaagttgagg aagttgttgt cggtggcgtt gatgaagaac tgcgaactcg ccgaatgcgg
     4621 cgccgaggtc cgcgccatcg ccagcgtgta gcggttgttc ttcaggccgt tgttggcctc
     4681 gttggtgatc gtcgcccgcg tggccttctg gcgcatgccc ggctcgaaac cgccgccctg
     4741 gatcatgaag ttgccgatga cgcggtggaa cacggtgttt tcgtagtggc ccgccttgac
     4801 gtactcaatg aagttggcaa cggtcaatgg tgccttttcg gcgttcagtt gcaggacgat
     4861 gtcgccgtgg ttggtggtca gtttgacttg agtcatgatc ggtactcttt caaggaattc
     4921 gtgggtttcg gggctgtgat gcccgcaacc ggtctggcgg taatgggcca ggttgggcag
     4981 tttagcgtgc cgggcacgaa tttcgaggct gtttttcatc tggacgtcga ttaaattcaa
     5041 tcgtttgccg gcctggcctg tcaggtgctt gacagcatcg gctatgatac gggctttgat
     5101 ttatcaggcc gcacccggcc gcgcacttgt acgttcaagg atcctatgag caagcccact
     5161 gtcgacccta cctcgaattc caagaccggc ccggccgtgc cggtcaattt cctgcgcccg
     5221 atcatccagg cggacctgga ctcgggtaag cacacacaga tcgtcactcg cttccctccg
     5281 gagcccaacg gctacctgca catcggccat gccaagtcga tctgcgtgaa tttcggcctg
     5341 gcccaggagt tcggtggcgt cacgcacctg cgtttcgacg acaccaaccc ggccaaggaa
     5401 gaccaggaat acatcgacgc gatcgaaagc gacgtcaaat ggctgggctt cgaatggtcc
     5461 ggtgaagtgc gctacgcctc gcagtatttc gaccagttgc acgactgggc ggtggagctg
     5521 atcaagtccg gcaaggccta tgtcgacgac ctgacccctg aacaagccaa gcaataccgt
     5581 ggcagcctga ccgagccagg caaaaacagc ccgttccgcg accgcagcgt cgaagagaac
     5641 ctggacctgt tcgcccgcat gaaggccggt gaattcccgg acggcgcacg tgtgctgcgg
     5701 gccaagatcg acatggcctc gccgaacatg aacttgcgcg acccgatcat g
//
LOCUS       DATMJB010000664         4085 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_217760, whole genome shotgun sequence.
ACCESSION   DATMJB010000664 DATMJB010000000
VERSION     DATMJB010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217760"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..649
                     /locus_tag="VNU48_14085"
     CDS             74..649
                     /locus_tag="VNU48_14085"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWI12458.1"
                     /translation="MALGDAILACLTERPMTGYELAKTFDASIGFFWKANHQQIYREL
                     SKLRDRGHIRGREVVQSGKPNKLVYTLTPEGRAALRHWGAGPSTPPSIKDDLLVRLYA
                     LDCVDIEPLRADLMARLEYHRGRGDRFERLLNKRFPQGTATPADTGKLLGLRIGLRHE
                     RAVAEWCEEAIDALAAISHGPAAVSIDDGLT"
     gene            858..>4085
                     /locus_tag="VNU48_14090"
     CDS             858..>4085
                     /locus_tag="VNU48_14090"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12459.1"
                     /translation="MVAPESEPDEIMNSRVSWSVEGIDPSVRERAEAAARRAGMSLND
                     WLISTIGESTPPDFRTRQELPRQELPQQELPQQELARQEQRPMMPGEATREVADIHQR
                     LDSITRQIEQISRPAPRADAPRNNASRGDAPRGEPAVARQLNDAISRLDARLSQISNP
                     APARQAQIQDSQRQTDMVERAATQVYRQSPPLSPASFDFAIAEIAARQNELDGPAPRP
                     TPRSTPPMAPGPTVPGPTAPAMHPMPPSMSAAAPSVPAGPDFSSLERHLVKITSQIEA
                     LRRPDEIEHSIATFRGELAEIRNVITEAMPRRAIESIESEIRSLSRRIDDTRQNGTDG
                     QALAGIERALSEIREVLRSLTPAEQLAGYDDAIRNLGAKLDLILRANDDPSTVHQLES
                     AIAALRSIVSNVASNDALARLSDDVHTLSSKVDQISRAGDNSDSFAILEQRIAALTST
                     LESRARPVASENTEHIESALRALSDRLDRMPVGNDSASAFAHLEQRVSYLLERLESST
                     DHRSGNLGRVEDGLQDILRHLENQHANFAALAESARNAPTPVQQDTGLVDLIKRELSD
                     VRFSQSETDRRTQDSLETVHNTLGHVVDRLAMIEGDLRTVRSAPVATPQPTAIPAPAP
                     METAAPRAALPPQPKPEMPNPAAAQTHFASAPREFHAAEPAMPPAMPVPPRAISEILE
                     PHTTPSRAAIEPELPPDHPLEPGTRPSARAASPSERIAASENAISGIAPVTPDTNSTS
                     NFIAAARRAAQAAAATPANEKPSRPAKKVAADKKTAAKDPSKDGAKEPSTITSKIRSL
                     LVGASVVVIVLGTFKMAMTLLDSSSVPPMPAMPPMENSSEAPSPGNDVGKPATPAPAA
                     PSMTSPTPIGRQSFNNSMPNVPDSTASSVAIPQDAPAQASSPASASDITGAIPATQTS
                     SISRKLSLVAVPSTERLPDAIGGPVLRAAALKGDASAAYEIGVRFAEGKGVAPNLDEA
                     AKWYDRAAQAGVVPAVFRLGTFYEKGLSVKKDVDIARRYYVQAAERGNAKAMHNLAVL
                     DADGGGKGANYKSASQWFRKAADRGVADSQYNLGILYARGIGVEQN"
BASE COUNT          792 a         1464 c         1236 g          593 t
ORIGIN      
        1 ttatgcgcat tgacgcatat gcaactttgt gcataatgtc aatgcggatt cattataact
       61 gagggttggc ggagtggcgc tcggtgacgc gatcctggcg tgcctgacgg aacgtccgat
      121 gacgggctat gaactcgcca agacattcga tgcgtccatc gggttcttct ggaaagccaa
      181 tcaccagcaa atctatcgcg aactttccaa gcttcgggat cgcggccaca tccggggccg
      241 cgaggtggtg cagtccggca agcccaacaa actggtctac accctgacgc cggaaggccg
      301 ggccgcgctg cggcactggg gcgccgggcc gagcaccccg ccctcgataa aggacgatct
      361 gctggtgcgg ctttacgcgc tcgactgcgt cgatatcgag ccgctacgcg ccgacctgat
      421 ggcgcggctg gagtaccatc gcggccgcgg cgaccgcttc gaacgccttc tcaacaagcg
      481 cttcccgcaa ggcacggcga cgccggccga taccggcaag ctgctcgggc tacgcatcgg
      541 cctgcgtcac gaacgcgccg tggccgagtg gtgcgaagag gcgatcgatg ccttggccgc
      601 gatctcgcac ggtccagccg cggtgtcgat cgatgacggg ctaacctgag cagcaggctg
      661 tcggacccgg tcctcacgac cctgcccgcg accgggcaaa ccccgccgat gtcgccgacc
      721 ggccaacttt aagaccgccc gccctaattc cttaaccggt tatttaccgt aacgcgaaaa
      781 agtcgaatcc agaggcagac gacctgcgcg gaattcgatt gtctcgtcag tgggacgcgc
      841 ggggaagctg aggcgcaatg gtcgcgccgg agtcggaacc ggacgaaatc atgaattcgc
      901 gcgtatcgtg gagtgttgaa ggcatcgatc catccgtgcg cgagcgtgcc gaagccgctg
      961 cgcgccgcgc cggcatgtca ctgaacgact ggctcatctc caccatcggc gaatccaccc
     1021 ctcccgactt ccgcacacgc caggaacttc ctcggcaaga acttccacaa caagaacttc
     1081 cacagcaaga acttgcacgg caggaacagc ggccgatgat gccgggcgag gcaacccgcg
     1141 aggttgccga cattcatcag cggctcgatt cgattacccg ccagatcgaa caaatctccc
     1201 gtcctgcccc gcgcgcggat gcgccacgca ataatgcatc tcgtggcgat gcccctcgcg
     1261 gcgagcccgc tgtggcccgc cagttgaacg acgcgatttc acggctcgat gcccggctgt
     1321 cgcaaatctc gaaccctgcg ccggcacggc aggcccagat acaggacagc cagcgccaga
     1381 cagacatggt cgagcgcgcg gcaacgcaag tctatcggca gtcgccgcca ctcagcccgg
     1441 cttcgttcga ctttgcgatc gcggaaatcg cggcgcgcca gaacgaactc gatggcccgg
     1501 caccccggcc gacgccacgc agcacgccgc cgatggcgcc cgggccgaca gtgcccgggc
     1561 cgacagcgcc ggccatgcat ccgatgccgc cttcaatgtc agcggccgca ccttcggtgc
     1621 cggcaggccc ggacttctcg tcactggaac gccatctggt caagatcacc agccagatcg
     1681 aggcgctgcg gcgccctgac gaaatcgaac attcgatcgc gacattccgc ggcgaactgg
     1741 cggaaattcg caacgtcatc accgaggcga tgccgcgccg ggcgatcgaa tcgatcgaga
     1801 gcgaaatccg ctcgctgtcg cgccgcatcg acgacacccg tcagaacggc accgacggcc
     1861 aggcgctggc cggcatcgaa cgcgcgctct ctgaaattcg cgaagtgctg cgctcgctca
     1921 ccccggccga gcagctggcc ggctacgacg acgcgatccg caatctcggc gccaagctcg
     1981 atctgatcct gcgcgccaac gacgatccct cgacggtcca tcaactcgaa agcgctatcg
     2041 cagcccttcg ctccatcgtc tccaatgtcg cctccaacga cgcgctggcg cggctttccg
     2101 acgacgtgca cacgctgtcg tccaaagtcg atcagatctc gcgcgccggc gacaacagcg
     2161 attccttcgc gatcctggaa cagcgcatcg ccgccctgac ctcgacgctg gaaagccgcg
     2221 cgcggccggt agcgagcgag aacaccgaac atatcgaaag cgcgctgcgc gcgctgtccg
     2281 accgcctcga ccgcatgccg gtcggcaacg acagcgcgtc cgccttcgcg cacctcgaac
     2341 agcgcgtgtc gtatctgctc gagcgcctcg aaagctccac cgatcaccgg tccggcaatc
     2401 tcggacgggt cgaggacggg ctgcaggaca tcctgcgcca tcttgaaaac cagcatgcca
     2461 acttcgccgc gctggccgag agcgcccgca acgcgcccac gccggttcag caggacaccg
     2521 gcctggtcga cctgatcaag cgcgaactgt ccgacgtccg tttcagccag tccgaaaccg
     2581 atcgccgcac ccaggattcg ctggaaaccg tacacaacac gctcggccat gtggtcgatc
     2641 gcctcgccat gatcgagggc gatctgcgca cggttcgttc cgcacccgtc gcaacaccgc
     2701 agccgacggc gatacccgct ccggcgccga tggaaaccgc agcgccgcgc gccgcgctgc
     2761 cgccgcagcc caaacccgaa atgccgaatc cggccgcggc gcagacgcat ttcgcctcgg
     2821 ccccgcgcga attccacgcc gccgagccgg ccatgccgcc cgcgatgccg gtgccgccgc
     2881 gcgccatcag cgaaattctg gaaccgcata cgacgccgtc gcgcgctgcg atcgaaccgg
     2941 aattgccgcc cgaccatccg ctcgaacccg gcacccgccc gtcagcgcgc gcggcgtcac
     3001 cctcggagcg catcgccgcc tcggagaatg cgatcagcgg cattgcgccg gtgacacccg
     3061 acaccaacag cacctcgaat tttatcgccg ccgcgcgccg cgctgcccag gccgccgccg
     3121 cgacacccgc caacgagaag ccctcgcgtc cggccaagaa ggtcgccgcc gacaagaaaa
     3181 ccgctgccaa ggatccatcg aaggatgggg ccaaggagcc atcgaccatt acctccaaga
     3241 tccgttcgct gctggtcggc gcgagcgtgg tcgtgatcgt gctcggcacc ttcaagatgg
     3301 cgatgacgtt gctcgacagc agcagcgtgc cgccgatgcc cgcgatgccg ccgatggaga
     3361 attccagcga agcaccctcg cccggaaacg atgtcggcaa gccagccacg ccggctcccg
     3421 ccgcgccttc gatgacatcg ccgacgccga tcggccggca atcgttcaac aattcaatgc
     3481 cgaacgtccc cgacagcacg gcgtcgtcgg tggcgatccc gcaagatgct ccggcacagg
     3541 cttcctcacc ggcatcggcc agcgacatca ccggcgcaat tccggccacg cagacctcgt
     3601 cgatcagccg caaactctcg ttggtggcgg tgccgtcgac cgaaaggttg ccggacgcga
     3661 tcggcggacc ggtgctgcgc gcggccgcgc tgaagggcga tgcaagcgcg gcctacgaga
     3721 tcggcgtgcg cttcgccgaa ggcaaaggcg tcgcccccaa tctggacgaa gccgccaaat
     3781 ggtacgaccg cgcggcgcag gccggcgtgg tgcccgcggt gttccggctc gggaccttct
     3841 acgaaaaggg cctgagcgtg aaaaaggacg tcgacatcgc gcggcgttac tacgtgcagg
     3901 cggccgagcg cggcaacgcc aaggccatgc acaatctggc ggtgctggat gccgacggcg
     3961 gcggcaaggg cgccaactac aagagcgcgt cgcaatggtt ccgcaaggcg gccgatcgcg
     4021 gcgtcgccga cagccagtac aacctcggca tcctctatgc ccgcggcatc ggcgtcgaac
     4081 agaac
//
LOCUS       DATMJB010000665         6582 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_859792, whole genome shotgun sequence.
ACCESSION   DATMJB010000665 DATMJB010000000
VERSION     DATMJB010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6582
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859792"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1352)
                     /locus_tag="VNU48_14095"
     CDS             complement(156..1352)
                     /locus_tag="VNU48_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HWI12460.1"
                     /translation="MEAYVLDWANLLLRWAHIITGIAWIGSSFYFVSLDNSLTPPKDA
                     KDAAKGVGGEQWGVHGGGFYHHQKYPVSPGYVPEQLHWSMWESYSTWLTGFALFTVLY
                     LYNASTFLIDKTLFDWTPTAAICTALAFFVAFWVLYDTICRTLGRGKHGDEIVGGCVF
                     VLIVLASWLSCHLFAGRAAFLLVGAMIATTMSGNVFFWIIPGQRTVVAAIKAGAAVDP
                     VHGQRGKQRSVHNTFFTLPVLFSMMSNHYGFTYGARYNWLVLVAIMVAGALIRMSFAL
                     RHRALADGRPVPWHFAGIGSALLLGVIVATAPAPQAPQAAAAPASFAEVQAIVAQRCV
                     LCHGPAMQSKNLRFDSPDEITRHAQQIYQQAVVLKLMPMNNATQITDAERALIGRWFT
                     AGAPTK"
     gene            complement(1566..1919)
                     /gene="uraH"
                     /locus_tag="VNU48_14100"
     CDS             complement(1566..1919)
                     /gene="uraH"
                     /locus_tag="VNU48_14100"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HWI12461.1"
                     /translation="MGKLTTHVLDTANGCPAAGMAVALYRLDAGTPTLLKRITLNHDG
                     RADSPLLEGASLKPGTYRLVFGVAAYFRGLGVALAEPPFLDEVPLDFGLAAPGSHYHV
                     PLLASPFAYSTYRGS"
     gene            2033..2779
                     /locus_tag="VNU48_14105"
     CDS             2033..2779
                     /locus_tag="VNU48_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWI12462.1"
                     /translation="MPRPAPKLQVIRPGRAAAPRAADPAASETLTRRIADSITTAIIE
                     RRLMPGTKLAEQKIADVFKVSRTLVRQALMQLSRDHLITLEPARGARVAEPSVDEAKQ
                     VFDARNMLEAAMIRRAAAEFGPAQIAELRAHLKAESLALQRTDVPGRTRLLADFHVVI
                     ARMLGNEVLAHMLGDLVSRCSLIALMYQSAHSAEHSYDEHVAIVDALEKRDARAAVRL
                     MESHLNNVERNLRLDPRAPDLGALLLPEGL"
     gene            2807..3742
                     /gene="puuE"
                     /locus_tag="VNU48_14110"
     CDS             2807..3742
                     /gene="puuE"
                     /locus_tag="VNU48_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HWI12463.1"
                     /translation="MTKDYPRDLIGYGRNPPHAQWPGNARVAVQFVLNYEEGGENCVL
                     HGDPSSETFLSEIIGAQAFEMRHLSMESMYEYGSRAGVWRILKEFEKRGLPLTVFGVS
                     MALQRNPELTDAFVELGHEIACHGWRWIHYQHMGEERERRHIELGTQIIRDMTKGQWP
                     LGWYTGRDSPNTKRLVADHGGYEYDSDYYGDDLPFWMPVDKSDGTTLQRLIVPYTLDT
                     NDMRFAAMQGFNTGDHFFTYLKDAFDVLYAEGDPDGQDRPKMLSIGMHCRILGRPGRF
                     RSLQRFLDHVQGHERVWISRRIDIARHWAATHPAP"
     gene            3768..5552
                     /gene="uraD"
                     /locus_tag="VNU48_14115"
     CDS             3768..5552
                     /gene="uraD"
                     /locus_tag="VNU48_14115"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HWI12464.1"
                     /translation="MASPLTLDRLNRAAPAEFATLLDGVYEHSPWIAERAAAKRPFAS
                     LAQLKHALVTVLREAGRDAQIALIRAHPELAGKAMVAKTLTAESTHEQGRAGLAECTP
                     AEFAHLHALNAAYHKKFGWPFTLAVRGPRGSGLTRHEIIRTFERRLAGHPDFELAECV
                     RNIHRIAEIRLNDKFGVEPTLGNQAWDCAELLARHTDPGYGERNELTVTYLTDAHRAC
                     ARQLEAWMRECGFDEVSIDAVGNVVGVYRGSDPTRQRLLTGSHYDTVRNGGKYDGRLG
                     IFVPMICVRELHRAGKRLPYGVEVVGFAEEEGQRYKATFLGSGALTGHFDPAWLEQRD
                     ADGITMRDAMRLAGLPATLPAIHALKRDAADYQGFVEVHIEQGPVLNALDLPLGVVTS
                     INGGVRYLGEVTGMASHAGTTPMDSRRDAAVAVAELALFLEKRAGAAPNLVGTMGILN
                     VPNGSTNVVPGRCQFTLDIRATTNEVRDACAADVLTELARICERRGLNHSIEETMRAA
                     AAPSAPAWQQRWERAVEAGGLPVFRMPSGAGHDAMKLHEAMPQAMLFVRGQNAGISHN
                     PLESSTSDDMQLAVDAFMHLLNQLATET"
     gene            5563..>6582
                     /locus_tag="VNU48_14120"
     CDS             5563..>6582
                     /locus_tag="VNU48_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWI12465.1"
                     /translation="MTAYEQLDAWIDAHFDEEVTFLQALVQVPTDTPPGNNAPHAERS
                     AELLKAFGFEAEKHAVPDADVKAYGLTSLTNLIVRRPYAPGGKTIALNAHGDVVPPGD
                     GWTHGPYSGEIVDGKLYGRASAVSKSDFATYTFAVRAIEQTLRSQLTGAVELHFTYDE
                     EFGGELGPGWLLRNKLTKPDLLLAAGFSYQVVTAHNGCLQMEVTVHGKMAHAAIPHTG
                     IDALQGAVAILNALYRQNTLYQRVTSKVEGITHPYLNVGRIEGGTNTNVVPGKVVLKL
                     DRRMIPEENPAEVEATIRQVIQDAAGRPGISVEIKRLLLANSMQPLPGNQPLVDALQR
                     HGEAVF"
BASE COUNT         1119 a         2335 c         2215 g          913 t
ORIGIN      
        1 tcgggcaggt cgccggtggg gccgaccgcc tcgatcacgt tgtcacgcac gaacaccgca
       61 ccgtccgcga tctcgcggcg ctgtgcgtcc atcgtgacga gcagcgtggc gttgcggatc
      121 aacagggtcg acatcgcgcg ctcagtgggc ctcgatcact tcgtcggcgc gccggccgtg
      181 aaccagcgcc cgatcagcgc gcgttcggcg tcggtgatct gggtcgcgtt gttcatcggc
      241 atcagcttca gcaccaccgc ctgctggtag atctgctgcg cgtgcctggt gatctcgtcg
      301 ggcgagtcga agcgcaggtt cttgctctgc atcgccgggc cgtggcacag cacgcagcgc
      361 tgcgccacga tcgcctgcac ctcggcgaag ctcgccggcg cggccgccgc ctgcggtgcc
      421 tgcggcgccg gcgcggtggc gacgatcacg cccagcagca gcgcgctgcc gatgcccgcg
      481 aagtgccacg gcaccggcct gccgtcggcc agcgcgcggt ggcgcagcgc gaacgacatg
      541 cggatcaacg cgcccgccac catgatcgcc accagcacca gccagttgta cctggcgccg
      601 taggtgaagc cgtagtggtt gctcatcatc gagaacagca ccggcagcgt gaagaaggtg
      661 ttgtgcacgc tgcgctgctt gccgcgctgg ccgtgcaccg ggtctacggc cgctccggcc
      721 ttgatcgcgg cgaccaccgt gcgctggccc gggatgatcc agaagaacac gttgccgctc
      781 atcgtcgtcg cgatcatcgc gccgaccagc aggaacgccg cgcgcccggc gaacaggtgg
      841 cacgacagcc agctcgcgag cacgatcagc acgaacacgc acccgccgac gatctcgtcg
      901 ccatgcttgc cgcggccgag cgtgcggcag atcgtgtcgt acagcaccca gaacgcgacg
      961 aagaacgcca gcgcggtgca gatcgccgcg gtcggcgtcc agtcgaagag cgtcttgtcg
     1021 atcaggaagg tgctggcgtt gtacaggtac agcaccgtga agagcgcgaa gccggtgagc
     1081 caggtcgagt agctctccca catcgaccag tgcagctgct cgggcacgta gccgggcgag
     1141 accgggtact tctggtggtg atagaagccg ccgccgtgca cgccccactg ctcgccgccg
     1201 acgcccttgg ccgcgtcctt ggcgtccttc ggcggcgtca ggctgttgtc gagcgagacg
     1261 aagtagaacg atgagccgat ccaggcgatg cccgtgatga tgtgggccca gcgcagcagc
     1321 aggttcgccc agtcgagaac gtacgcttcc atgcggtgtg agtctccgtg tcgtcgccag
     1381 gccggtgctg cgtttcagca agatgcaggc ctgtatcgct cccttgccac ggcgggctct
     1441 gcaaggtgtg aacgtcacga ccgggtgggc gaacactccg ggctccgcgg cgacgcgatg
     1501 aaagtgtata cagttcaagt cgatcatcgc atacgcattt accctgatcc gaccggcccg
     1561 acgggtcagg agccgcgata ggtcgagtac gcgaacgggc tcgccagcaa gggcacgtgg
     1621 tagtgcgaac ccggcgcggc gaggccgaag tccagcggca cctcgtccag gaacggcggc
     1681 tcggccagcg ccacgcccag gccgcggaag tacgccgcga cgccgaacac cagccggtag
     1741 gtgccgggtt tgaggctcgc gccctccagc agcgggctgt cggcgcggcc gtcgtggttc
     1801 agcgtgatgc gcttcagcag ggtcggagtg ccggcgtcga gccggtacag cgcaaccgcc
     1861 atgccggcgg ccgggcagcc gttggcggtg tcgagcacgt gagtggtgag cttgcccatg
     1921 gagtcctcgg tggcgcggtg cgcgtgggtg gcggcaagca aatagtgctt gccgtgctgg
     1981 caaaagtgta tacactttaa gccgcaacgc aagcccgatt tcagccgacg ccatgccccg
     2041 ccccgccccg aaactgcagg tcatccgccc cggtcgcgcc gcggccccgc gtgcggccga
     2101 cccggcggcg agcgaaacgc tgacgcggcg catcgccgac tcgatcacca ccgcgatcat
     2161 cgagcgccgc ctgatgccgg gcaccaagct ggccgagcag aagatcgccg acgtcttcaa
     2221 ggtctcgcgc acgctggtgc gccaggcgct gatgcagctc agccgggacc atctgatcac
     2281 gctcgagccg gcgcgcggcg cgcgcgtcgc cgaacccagc gtcgacgagg cgaagcaggt
     2341 cttcgacgcc cgcaacatgc tcgaggccgc gatgatccgg cgcgccgcgg ccgaattcgg
     2401 cccggcgcag atcgccgagc tgcgcgcgca cctgaaggcg gaaagcctgg cgctgcagcg
     2461 caccgacgtg ccgggccgca cccggctgct ggccgacttc cacgtcgtga tcgcgcgcat
     2521 gctgggcaac gaggtgctgg cgcacatgct gggcgatctg gtctcgcgct gctcgctgat
     2581 cgcgctgatg taccagtcgg cgcactcggc cgagcattcg tacgacgagc atgtcgcgat
     2641 cgtcgacgcg ctcgagaagc gcgatgcgcg tgccgcggtg cggctgatgg agtcgcacct
     2701 gaacaacgtg gaacgcaacc tgcggctcga cccgcgcgcc cccgacctgg gtgcgctgct
     2761 gctgcccgaa ggcctctgag gccgcctgcg gagaacccca aagatgatga ccaaggacta
     2821 cccgcgcgac ctgatcggct atggccgcaa cccgccgcac gcgcaatggc ccggcaacgc
     2881 gcgcgtcgcg gtccagttcg tgctgaacta cgaggaaggc ggcgagaact gcgtgctgca
     2941 cggcgacccg tcgtccgaga ccttcctgtc cgagatcatc ggcgcgcagg ccttcgagat
     3001 gcggcacctg tcgatggaat cgatgtacga gtacggctcg cgcgccggcg tctggcgcat
     3061 cctgaaggag ttcgagaagc gcggcctgcc gctgacggtg ttcggcgtct cgatggcgtt
     3121 gcagcgcaac ccggagctga ccgacgcgtt tgtcgagttg ggccacgaga tcgcctgcca
     3181 cggctggcgc tggatccact accagcacat gggcgaggaa cgcgagcgcc gccacatcga
     3241 gctcggcacg cagatcatca gggacatgac caagggccag tggccgctgg gctggtacac
     3301 cgggcgcgac agcccgaaca ccaagcggct ggtcgccgac cacggcggct acgagtacga
     3361 cagcgactac tacggcgacg acctgccgtt ctggatgccg gtggacaaga gcgacggcac
     3421 gacgctgcag cgcctgatcg tgccctacac gctcgacacc aacgacatgc gcttcgccgc
     3481 gatgcagggc ttcaacaccg gcgaccactt cttcacctac ctgaaggacg cgttcgacgt
     3541 gctctacgcc gaaggcgacc ccgatggtca ggatcggccg aagatgctga gcatcggcat
     3601 gcactgccgc atcctcggca ggcccgggcg cttcaggtcg ctgcagcgtt tcctcgatca
     3661 cgtgcagggc cacgagcgcg tctggatcag ccggcgcatc gacatcgcgc gccactgggc
     3721 cgccacgcac ccggcgccct gatcgaccca cccggagatc ttcattcatg gcttcgccac
     3781 tgacgctgga ccgactcaac cgcgccgcgc ccgccgaatt cgccacgctg ctggacggcg
     3841 tctacgaaca ctcgccgtgg atcgccgaac gcgccgccgc gaagcgcccg ttcgcgagcc
     3901 tcgcgcagct caagcacgcg ctcgtgacgg tgctgcgcga ggccgggcgc gacgcgcaga
     3961 tcgcgctgat tcgcgcccac cccgagctgg ccggcaaggc gatggtcgcg aagacgctga
     4021 ccgccgagtc gacccacgag cagggccgcg ccggcctggc cgaatgcacg ccggccgagt
     4081 tcgcgcacct gcacgcgctg aacgcggcct accacaagaa attcggctgg ccgttcacgc
     4141 tcgcggtgcg cggcccgcgc ggcagcggcc tgacgcgcca cgagatcatc cggaccttcg
     4201 agcggcgcct ggccgggcac ccggacttcg agctcgccga gtgcgttcgc aacatccacc
     4261 gcatcgccga gatccggctg aacgacaagt tcggcgtcga gccgacgctc ggcaaccagg
     4321 cctgggactg cgccgagctg ctcgcgcgcc acaccgaccc cggctacggc gagcgcaacg
     4381 aactgaccgt cacctacctg accgatgcgc accgtgcctg cgcgcgccag ctcgaggcct
     4441 ggatgcgcga atgcggtttc gacgaggtct cgatcgacgc ggtcggcaac gtcgtcggcg
     4501 tctatcgcgg cagcgacccg acgcgccaac gcctgctgac cggctcgcac tacgacaccg
     4561 tgcgcaacgg cggcaagtac gacgggcgtc tgggcatctt cgtgccgatg atctgcgtgc
     4621 gcgaactgca ccgcgccggc aagcggctgc cttacggcgt cgaggtcgtc ggcttcgccg
     4681 aggaggaagg ccagcgctac aaggcgacct tcctcggctc gggcgcgctg accggccact
     4741 tcgacccggc ctggctggag cagcgcgacg ccgacggcat cacgatgcgc gacgcgatgc
     4801 gcctggccgg cctgcccgcg accttgccgg cgatccacgc gctgaagcgc gacgcggccg
     4861 actaccaggg tttcgtcgag gtccacatcg agcaggggcc ggtgctgaac gcgctcgacc
     4921 tgccgctggg cgtggtcacc tcgatcaacg gcggcgtgcg ctacctcggc gaagtgaccg
     4981 gcatggccag ccatgccggc accacgccga tggacagccg gcgcgacgcc gccgtcgccg
     5041 tcgccgaact cgcgctgttc ctcgagaagc gcgccggcgc cgcgccgaac ctggtcggca
     5101 cgatgggcat cctgaacgtg ccgaacggct cgaccaacgt cgtgccgggg cgctgccagt
     5161 tcacgctcga catccgcgcg acgaccaacg aggtgcgcga tgcctgcgcc gccgacgtgc
     5221 tcaccgaact cgcgcgcatc tgcgagcgcc gcggcctgaa ccacagcatc gaggagacga
     5281 tgcgcgccgc cgccgcgccg agcgccccgg cctggcagca gcgctgggag cgcgcggtcg
     5341 aggccggcgg gctgccggtg ttccgcatgc ccagcggcgc cggccacgac gcgatgaaac
     5401 tccacgaggc gatgccgcag gcgatgctgt tcgtgcgcgg ccagaacgcc ggcatcagcc
     5461 acaacccgct cgaatcgagc accagcgacg acatgcagct cgcggtcgat gcgttcatgc
     5521 acctgctgaa ccagctcgcc accgaaacat gagagtgtcc cgatgaccgc ctacgaacaa
     5581 ctcgacgcct ggatcgacgc gcacttcgat gaagaagtga ccttcctgca ggcgctggtg
     5641 caggtgccca ccgacacgcc gcccggcaac aacgcgccgc acgccgagcg cagcgccgag
     5701 ctgctgaagg ccttcggctt cgaggccgag aagcacgcgg tgcccgatgc cgacgtgaag
     5761 gcctacggcc tcacgtcact gacgaacctg atcgtgcggc gcccgtatgc gccgggcggc
     5821 aagacgatcg cgctgaacgc gcacggcgac gtcgtgccgc ccggcgacgg ctggacgcac
     5881 gggccgtaca gcggcgagat cgtcgacgga aagctctacg gtcgcgcgtc ggcggtcagc
     5941 aagagcgact tcgcgaccta caccttcgcg gtgcgcgcga tcgaacagac gctgaggtcg
     6001 caactcaccg gcgcggtcga actgcacttc acctacgacg aggagttcgg cggcgaactc
     6061 ggccccggct ggctgctgcg caacaagctc accaagcccg acctgctgct cgcggccggc
     6121 ttcagctacc aggtcgtcac ggcgcacaac ggctgcctgc agatggaagt cacggtgcac
     6181 ggcaagatgg cgcacgcggc cattccccac accggcatcg acgcgctgca gggcgcggtc
     6241 gcgatcctga acgcgctgta ccgccagaac acgctgtacc agcgggtcac gtccaaggtc
     6301 gagggcatca cgcacccgta cctgaacgtc ggccgcatcg aaggcggcac caacaccaac
     6361 gtcgtgcccg gcaaggtcgt gctcaagctc gaccgccgca tgatccccga ggagaacccg
     6421 gccgaggtcg aggcgacgat ccgccaggtc atccaggacg cggccggccg ccccggcatc
     6481 agcgtcgaga tcaagcgcct gctgctggcc aactcgatgc agccgctgcc aggcaaccag
     6541 ccgctggtcg acgcgctgca acggcatggc gaggcggtgt tc
//
LOCUS       DATMJB010000666         4850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_449727, whole genome shotgun sequence.
ACCESSION   DATMJB010000666 DATMJB010000000
VERSION     DATMJB010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449727"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VNU48_14125"
     CDS             <1..184
                     /locus_tag="VNU48_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347797.1"
                     /note="CcoO; FixO; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HWI12466.1"
                     /translation="PWLLTAPVDDTSIAAHMTGLRKVGVPYSDAEIAKGAADVRGKTE
                     LDALVAYLQVLGTAVK"
     gene            198..365
                     /locus_tag="VNU48_14130"
     CDS             198..365
                     /locus_tag="VNU48_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HWI12467.1"
                     /translation="MDVNDLRIGVTVLSFVAFMGIVAWALSRKNAARFADAAQLPFRE
                     VEASAEKGSQP"
     gene            362..1282
                     /gene="ccoP"
                     /locus_tag="VNU48_14135"
     CDS             362..1282
                     /gene="ccoP"
                     /locus_tag="VNU48_14135"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HWI12468.1"
                     /translation="MSDFVNSGWSIFVAALTIGGLLGCLWLLFVASRRQPMAADNSTG
                     HVFDEDLVEMNNPLPRWWMWLFILTVVFAFAYVAMFPGLGSFAGRLNWSSANQLEADK
                     QAAALQTAPIYAAFKSLPAAELSHNPKAMAIGERLFSNNCAACHGSDARGSKGFPNLT
                     DNDWLHGGTPEKIEETITQGRVGMMPPMAAAVGTPEDVRNVANYVLSLSGSPHNAIAA
                     AAGRAKFTVCAACHGADGKGNQALGAPNLTDKIWLHGWGEDAIVAMVTQGKTNTMPAQ
                     HGRLTPDQIHVLATYVWSLSQSALPVAVNK"
     gene            1287..2744
                     /gene="ccoG"
                     /locus_tag="VNU48_14140"
     CDS             1287..2744
                     /gene="ccoG"
                     /locus_tag="VNU48_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HWI12469.1"
                     /translation="MPAVGAPKKIIPIAVADPAGETVSLYQSEARIQMRSVTGWFAGW
                     RWALVWITQLVFYGTPWLQWSGRQAVLFDLGARRFYIFNLVLYPQDVIYLAGLLILSA
                     YALFLFTAVAGRLWCGFACPQTVYTELFMWVERRFEGDRQARIRLDAAPWSLNKLWRR
                     GGKQLAWVAIGLWTGISFVAYFTPARSLVVEAASLTLGPWEWFWSLFYGFATYGNAGY
                     MREQVCKYMCPYARFQSAMFDRDTLIISYDATRGEPRGSRPRGVEAASVGKGDCINCT
                     LCVQVCPTGIDIRDGLQYECIGCTACIDVCNGVMDKMGSPRGLIRYATQNGMEGRWDA
                     RRMWRRVLRPRVLVYTGVLLLIAGGFAASLALRAPFRADVVRDRGTLARMVEDGRIEN
                     VYRIQLMNATEEEQHFRIEIDGLPDAVIAAHGEIEVGPTQARWVPVAVQIPPETARSL
                     GGGAHPIRFRIALREHDHDRTRAELTEKSTFVVPR"
     gene            2764..3012
                     /locus_tag="VNU48_14145"
     CDS             2764..3012
                     /locus_tag="VNU48_14145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein FixH"
                     /protein_id="HWI12470.1"
                     /translation="MNTPSAIDTLPAVVAVPWWRVGVMWLVVGGLGVVVAGSFGLLAA
                     ALRDADAVVPQELTRVASPKAPHAPALQARNHAATPAP"
     gene            3033..3296
                     /locus_tag="VNU48_14150"
     CDS             3033..3296
                     /locus_tag="VNU48_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12471.1"
                     /translation="MNVPMKQRVLQILWPAFLVAGVLEMLVFAVVDPADLHWFGGPAI
                     GWSAQAIYTVTFLIFWGAIATAGAMSALLAVESDTLNPESLRR"
     gene            complement(3383..4177)
                     /gene="fnr"
                     /locus_tag="VNU48_14155"
     CDS             complement(3383..4177)
                     /gene="fnr"
                     /locus_tag="VNU48_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HWI12472.1"
                     /translation="MQSPTRADAPAAIDASCELAPHNVVPIRVQALRVACSSCNLREL
                     CLPVGLSDGELNRLDALVETRRSLRRGDALYSAGEPFRSLYAVRTGFFKTRVTAEDGR
                     DQVTGFQMAGELLGFDGIGTELHTCDAVALEDSQVCVIPFGQLESLSRQFGGLQRQLH
                     KVMSREIVRDHGVMLLLGSMRAEERLAAFLLNLTQRLQARGFSASALVLRMTREEIGS
                     YLGLKLETVSRAFSKFQDDGVLEVRQRQLRVLDEEALRQIVNGSNC"
     gene            4263..>4850
                     /locus_tag="VNU48_14160"
     CDS             4263..>4850
                     /locus_tag="VNU48_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWI12473.1"
                     /translation="MNAVTFAGDPVISAELLARLDGPGPRYTSYPTADRFVDAFGPAD
                     YERALQSRVQGAARNAGVAALSLYVHIPFCESVCYYCACNKIITRHHERATPYLDALE
                     VEIGLHVVELGVRQPVTQLHLGGGSPTFLSDAELTRLMQMLRRAFAIAATAEISIEVD
                     PRTADPQRLRHLAELGFNRLSFGVQDFDADVQAAVH"
BASE COUNT          727 a         1706 c         1675 g          742 t
ORIGIN      
        1 cccctggctg ctgaccgcac cggtcgacga caccagcatc gccgcgcaca tgaccggcct
       61 gcgcaaggtc ggtgtcccgt acagcgacgc cgagatcgcg aagggcgccg ccgacgtgcg
      121 cggcaagacc gagctcgacg cgctggtggc ctatctgcag gtgctcggca ccgcggtgaa
      181 gtgaaggagt ctgcgagatg gatgtgaacg acctgcgcat cggagtgacc gtgctgtcct
      241 tcgttgcctt catgggcatc gtggcctggg cgctgtcgcg caagaacgca gcacgcttcg
      301 ccgatgcggc gcaactcccg ttccgcgagg tggaggcttc ggccgagaag gggtcgcaac
      361 catgagcgac ttcgtcaaca gcggctggtc gatcttcgtc gccgcgctca ccatcggcgg
      421 cctgctgggc tgcctgtggt tgctgttcgt cgcgagccgg cgccagccga tggccgccga
      481 caacagcacc ggccatgtct tcgacgaaga cctcgtcgag atgaacaacc cgctgccgcg
      541 ctggtggatg tggctgttca tcctgaccgt ggtgttcgcg ttcgcctatg tagcgatgtt
      601 ccccggcctg ggcagcttcg ccggccggct gaactggagc agcgcgaacc agctcgaggc
      661 cgacaagcag gccgcggccc tccagaccgc gcccatctac gcggcgttca agagcctgcc
      721 ggccgccgag ttgagccaca acccgaaggc gatggcgatc ggcgaacgcc tgttcagcaa
      781 caactgcgcg gcctgccacg gctcggacgc gcgcggcagc aagggcttcc cgaacctgac
      841 cgacaacgac tggctgcacg gcggcacgcc ggagaagatc gaggagacga tcacgcaggg
      901 gcgcgtgggc atgatgccgc cgatggccgc cgcggtcggc acgccggaag acgtgcgcaa
      961 cgtcgcgaac tacgtgctga gcctgtcggg cagcccgcac aacgcgatcg ccgccgccgc
     1021 gggccgcgcc aagttcacgg tctgcgcagc ctgccacggc gccgacggca agggcaacca
     1081 ggcgctgggc gcgcccaacc tgaccgacaa gatctggctg cacggctggg gcgaggacgc
     1141 gatcgtcgcg atggtcacgc agggcaagac caacacgatg ccggcgcagc acgggcgact
     1201 gacaccggac cagatccacg tgctcgccac ctacgtgtgg agcctgtcgc agagcgctct
     1261 gccggtcgcg gtcaacaagt agtcccatgc ccgcggtcgg cgcgccgaag aagatcatcc
     1321 ccatcgccgt cgccgacccg gcaggggaga ccgtctcgct ctaccagagc gaggccagga
     1381 tccagatgcg ctccgtcacc ggctggttcg ccggctggcg ctgggccctc gtgtggatca
     1441 cgcagctggt gttctacgga acgccgtggc tgcagtggag cgggcgccag gcggtgctgt
     1501 tcgacctcgg cgcgcgccgc ttctacatct tcaacctggt gctgtacccg caggacgtca
     1561 tctacctggc cgggctgctg atcctgtcgg cctacgcgct gttcctgttc accgcggtgg
     1621 ccgggcgcct gtggtgcggc ttcgcctgcc cgcagacggt ctacaccgag ctcttcatgt
     1681 gggtcgaacg gcgcttcgaa ggcgaccgcc aggcgcgcat caggctcgac gccgcgccct
     1741 ggagcctgaa caagctgtgg cgccgcggcg gcaagcagct cgcctgggtg gcgatcggcc
     1801 tgtggaccgg catcagcttc gtcgcctact tcacgccggc gcgcagcctg gtcgtcgagg
     1861 ccgcctcgct gacgctcggg ccatgggaat ggttctggag cctgttctac ggcttcgcga
     1921 cctacggcaa cgccggctac atgcgcgagc aggtctgcaa gtacatgtgc ccgtacgcgc
     1981 gcttccagag cgcgatgttc gaccgcgaca ccctgatcat cagctacgac gcgacgcgcg
     2041 gcgaaccgcg cggctcgcgc ccgcgcggcg tcgaggcggc ctcggtgggc aagggcgact
     2101 gcatcaactg cacgctgtgc gtgcaggtct gcccgaccgg catcgacatc cgcgacggcc
     2161 tgcagtacga atgcatcggc tgcaccgcgt gcatcgacgt gtgcaacggc gtgatggaca
     2221 agatgggctc gccgcgcggc ctgatccgct atgcgacgca gaacggcatg gagggccgct
     2281 gggacgcgcg ccgcatgtgg cggcgcgtgc tgcgtccgcg cgtgctggtc tacaccggcg
     2341 tgctgctgct gatcgccggc ggcttcgcgg ccagcctggc gctgcgcgcg ccgttccgtg
     2401 ccgacgtggt gcgcgatcgc ggcacgctgg cgcgcatggt cgaggacggc cgcatcgaga
     2461 acgtctaccg catccagctg atgaacgcga ccgaggagga gcagcatttc cgcatcgaga
     2521 tcgacgggct gcccgacgcc gtcatcgccg cgcacggcga gatcgaagtc ggcccgacgc
     2581 aggcgcgctg ggtgccggtc gcggtgcaga tcccgcccga gaccgcgcgc agcctgggcg
     2641 gcggcgcaca cccgatccgc ttccgcatcg ccctgcgcga acacgaccat gaccgcacgc
     2701 gcgccgaact gaccgaaaag tccaccttcg tggtgccgcg ctgaaccgaa caggagccgc
     2761 tcgatgaaca ctccctccgc gatcgacacg ctccccgccg tcgtcgccgt gccctggtgg
     2821 cgcgtcggtg tcatgtggct ggtggtgggc ggcctgggcg tcgtggtcgc cggcagcttc
     2881 ggcttgctgg ccgccgcgct gcgcgacgcg gacgccgtcg tgccgcagga gctcacccgc
     2941 gtcgccagcc cgaaggcgcc gcacgcgccg gcgctgcagg cgcgcaacca cgcggccacg
     3001 ccggcgccgt gaacccttca ccgggagttc cgatgaatgt tccgatgaag cagcgcgtgt
     3061 tgcagatcct gtggccggcg ttcctggtcg ccggcgtgct ggagatgctg gtgttcgcgg
     3121 tcgtcgaccc ggccgatctg cactggttcg gcggcccggc gatcggctgg tcggcgcagg
     3181 cgatctacac cgtcacgttc ctgatcttct ggggcgcgat cgcgacggcc ggcgcgatga
     3241 gcgcgctgct ggcggtcgag tcggacacgc tgaacccgga atcgctgcgg cgttgaccgg
     3301 acctctcaag caaatgtggc tttgccactt tgcttgaacc cccgcggggg gcgggctggg
     3361 cgcagcccgg ccctgggggc gctcagcagt tcgagccgtt gacgatctgg cgcagcgcct
     3421 cttcgtcgag cacgcgcagc tggcgctgcc tgacctcgag cacgccgtcg tcctggaact
     3481 tcgagaacgc gcggctcacg gtctcgagct tcaggccgag gtagctgccg atctcctcgc
     3541 gcgtcatgcg cagcaccagc gccgaggccg agaagccgcg tgcctgcagc cgctgcgtca
     3601 ggttcagcag gaacgcggcg aggcgctcct cggcgcgcat gctgccgagc agcagcatca
     3661 cgccgtggtc gcgcacgatc tcgcggctca tgaccttgtg cagctggcgc tgcagcccgc
     3721 cgaactggcg cgacagcgat tcgagctggc cgaacgggat cacgcagacc tgcgaatcct
     3781 cgagcgcgac cgcgtcgcag gtgtgcagct cggtgccgat gccgtcgaag ccgagcaact
     3841 cgcccgccat ctggaagccg gtgacctggt cgcgcccgtc ctcggcggtg acgcgcgtct
     3901 tgaagaaccc ggtgcgcacg gcgtagagcg acctgaacgg ctcgccggcg ctgtacagcg
     3961 cgtcgccgcg gcgcagcgag cggcgcgtct cgaccagcgc gtccaggcga ttcagttcgc
     4021 cgtcgctcag gcccaccggc aggcacagct cgcgcaggtt gcagctcgag caggcgaccc
     4081 gcagggcctg cacacggatc ggcacgacgt tgtgcggtgc caattcgcac gaggcatcga
     4141 tcgctgcggg ggcatcggcg cgggttggac tttgcatgag atcaagtgtg tgccacttcg
     4201 cgcgcgcatt gcttgcgcca tgtcaagcgg cccgtgcgtc gcgcaaggca cagtcggcgg
     4261 acatgaatgc tgtcaccttt gccggcgacc cggtcatctc ggccgaactg ctggcccggc
     4321 ttgacgggcc ggggccgcgc tacacctcct acccgaccgc cgaccgcttc gtcgacgcct
     4381 tcgggccggc cgactacgaa cgcgcgttgc agtcgcgcgt gcagggcgcg gcgcgcaacg
     4441 ccggcgtggc cgcgctgtcg ctgtacgtgc atatcccgtt ctgcgagtcg gtctgctact
     4501 actgcgcctg caacaagatc atcacccgcc accacgagcg cgccacgccg tacctcgatg
     4561 cgctcgaggt cgagatcggc ctgcacgtgg tggagctcgg ggtgcgccag ccggtcacgc
     4621 aactgcacct gggcggcggc tcgccgacct tcctgagcga tgccgagctg acccggctga
     4681 tgcagatgct gcgccgcgcg ttcgcgatcg ccgcgaccgc cgagatctcg atcgaggtcg
     4741 acccgcgcac cgccgacccg cagcgcttgc gccacctcgc cgaactcggc ttcaaccgcc
     4801 tgagcttcgg cgtgcaggac ttcgacgccg acgtccaggc cgcggtgcac
//
LOCUS       DATMJB010000667         6738 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_58713, whole genome shotgun sequence.
ACCESSION   DATMJB010000667 DATMJB010000000
VERSION     DATMJB010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58713"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /gene="boxC"
                     /locus_tag="VNU48_14165"
     CDS             <1..1287
                     /gene="boxC"
                     /locus_tag="VNU48_14165"
                     /EC_number="4.1.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-epoxybenzoyl-CoA dihydrolase"
                     /protein_id="HWI12474.1"
                     /translation="SIKFIAAVNGACAGGGYELALACDEIVLVDDRSSSVSLPEVPLL
                     GVLPGTGGLTRLTDKRRVRHDHADIFCTTSEGIRGRRAKDWRLVDEVVKTQGFAQRVQ
                     ERARELAALSDRPADAKGVALTPLNKTVTADAINYGHVSVAIDRAGRSATLTVKAPSG
                     AQPTDVAGIEAAGSAWYPLQMARELEDAILHLRTNELDIGTWLLKTEGSVADVLAMDA
                     TLQANASHWFVRETIGLLRRTLARLDVSSRSMFALVERGSCFAGTLLEIALAADRSYM
                     LALPDEPETAPKIALSAMNFGTFPMVSQETRLQRRFYGDAGELAAVQATIGQSLDADA
                     ALALGLITSAPDDIDWPDETRIAIEERASMSPDALTGMEANLRFAGQETMETRIFGRL
                     TAWQNWIFIRPNAVGEGGALKVYGTGTKPQFDRNRV"
     gene            1300..2727
                     /gene="boxB"
                     /locus_tag="VNU48_14170"
     CDS             1300..2727
                     /gene="boxB"
                     /locus_tag="VNU48_14170"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HWI12475.1"
                     /translation="MSTVNYSDKIPNNVNLSEDRTLQRALEGWLPNYLDWWQDMGPSG
                     SQGFDVYLRTATSTTPDGWATFGHVKMPDYRWGIFLAAQENERKINFGEHKGEAAWQD
                     VPGEHRANLRRIIVTQGDTEPASVEQQRHLGITAPSQYDLRNLFQVNVEEGRHLWAMV
                     YLLHKHFGRDGREEAEALLERRSGDADNPRILGAFNEETPDWLAFFMFTYFTDRDGKF
                     QLNALAESGFDPLARTTKFMLTEEAHHMFVGESGVSRVIQRTCQVMKETGIEDPAELR
                     KKGVIDLPTIQRYLNFHYSVTIDLFGADQSSNAAIFYSAGLKGRFEETKRTDDHLLKG
                     EGYKILEVKDGVLVEKEVPMLNALNEVLRDDFIKDSLGGVNRWNKVIEKAGLGFRLTL
                     PHKAFNRHIGTFAGIRVSPDGRAISEAEWAANVGQWLATEEDRAYVACLMGRVVETGK
                     YANWIAPPAIGINKQPVDFEYIRFN"
     gene            2747..3982
                     /gene="boxA"
                     /locus_tag="VNU48_14175"
     CDS             2747..3982
                     /gene="boxA"
                     /locus_tag="VNU48_14175"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxA"
                     /protein_id="HWI12476.1"
                     /translation="MSVAEIVLVKQHLIDPEVCIRCNTCEETCPVDAITHDERNYVVN
                     AEVCNACGACLGPCPTGSIDHWRVVDRSKVFTLAEQFGWDELPSVEEAGATTSDDSLA
                     TLVGAEAPAQVNAAVSPSSTKPPYSAAVPQTNLHGVKNPITATVAGNLRLTGEGASGD
                     VRHIVLDFGSLPFPVLEGQAIGIIPPGVDANGRAHHPRMYSVASPRSGEREGYNNLSL
                     TVKRMTEGDDGQPFRGVCSNYVCDLKKGDTVQVTGPFGASFLMPNHAGSNLLMICTGT
                     GSAPMRAMTEQRRRLRAVGQPLPGKLMLFFGARRAEELPYFGPLTKLPADFIDTCLAF
                     SRVPGQPRTYVQDRLRERASDVAELLRGDTFVYLCGLKGMETGVLEALCDACTAAGMD
                     WPALHRRFVDEGRFHVETY"
     gene            4024..5772
                     /locus_tag="VNU48_14180"
     CDS             4024..5772
                     /locus_tag="VNU48_14180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12477.1"
                     /translation="MTRREWTIGLPIVVLLMLCLVAYLRGMALGNDAGALTSLRVFGP
                     PDAQKVAAVIGSRLHVLDAGGQRLADQDMRALGFSEDPNDMDWTVDAQGRTEAWFFED
                     TPPRVVRCAWSDAQTRLDNCASAIAGAKLKVNPASRAVHLAVDRPGERVFVTDAKGGA
                     VQMFDFSGKRLAKSDAGEVALDFPNRIRYLGDNTLLVGDNDHGRVVWLAAEPGKPIRL
                     LKTLYAQDHGGARLAHTKVTDAAISPSGTLWMLAMRFGQADGDILVFDANHQPVRRAA
                     LRGDADPVIVEPLGDALLVGDFSPPRLYRVDAQGRLLDDFGDAGFRADLLPLQAQAEH
                     ASLWMRAAAVGAVVTFVVGSLLAWRFSERPVPPGRIDAPALAGIERARAAAAPLRFPI
                     VIERSAEYLRQERRLLWAMAAATVALFGGVAWILVANGKHIDLSKISWRLWAQAVGMA
                     ALAPIAIAWTYWFGGRRSLQVTEHAVRWRSGERTVAEAPLHAVLASANALLIGTRMVL
                     IRQRTRARKPGTPMFDAALIEQSILARLPRENLVDDAALTREMLRRNVYLKALIVAAI
                     VGTILVIAHSLYRALG"
     gene            6177..>6738
                     /locus_tag="VNU48_14185"
     CDS             6177..>6738
                     /locus_tag="VNU48_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HWI12478.1"
                     /translation="MKPNTSSTPRIDLGRRSLIKAAAATGAAGLVPGLQSAVYAAGSD
                     KPEKEEVRIGFIPLTDCASVVMASVLGFDKKYGVKIIPTKEASWAGVRDKLVNGELDM
                     AHVLYGLIYGVHIGVGGPKKDMAVLMSLNNNGQAITLSKTLADKGAVDGPSLAKAMAK
                     DKREYTFAGTFPTGTHAMWMHYWLAAS"
BASE COUNT         1108 a         2329 c         2291 g         1010 t
ORIGIN      
        1 tctatcaagt tcatcgccgc ggtcaacggc gcctgcgccg gcggcggcta cgagctcgcc
       61 ctcgcgtgtg acgagatcgt cctcgtcgac gaccgctcgt cgtcggtctc gcttcccgaa
      121 gtgccgctgc tcggcgtgct gcccggcacc ggcggcctga cgcggctgac cgacaagcgc
      181 cgcgtgcgcc acgaccacgc cgacatcttc tgcaccacca gcgaaggcat tcgcggcaga
      241 cgagcgaagg actggcggct tgtcgacgag gtcgtgaaga cccaaggctt cgcgcagcgc
      301 gtgcaggagc gggcgcgcga gttggccgcg ctcagcgatc ggccggcaga cgcgaaaggc
      361 gtggcgctga cgccgctgaa caagaccgtc actgcggatg cgatcaacta cggtcacgtg
      421 tccgtcgcga tcgatcgcgc cggccgcagc gcgacgctga ccgtgaaggc gccgagcggc
      481 gcacagccga ccgatgtggc cggcatcgag gccgcgggtt cggcctggta cccgctgcag
      541 atggcgcgcg agctcgaaga cgcgatcctg cacctgcgca cgaatgagct cgacatcggc
      601 acctggctgc tgaagaccga agggtcggtc gccgacgtgc tcgcgatgga cgcgacgctg
      661 caggcgaatg catcgcactg gtttgtgcgc gagacgatcg gtctgctgcg ccgcacgctg
      721 gcgcggctcg acgtgtcgtc gcgctcgatg ttcgcgctgg tcgagcgtgg ttcgtgtttt
      781 gccggcacgc tgctcgagat cgcgctcgcc gccgaccgaa gctacatgct cgcgctgccc
      841 gacgaacccg agaccgcgcc gaagatcgcc ctctcggcga tgaacttcgg cactttcccg
      901 atggtgagcc aagagacgcg cttgcagcgt cgcttttacg gcgatgccgg tgagcttgcc
      961 gcggtgcagg caacgatcgg ccaatcgctg gacgccgatg cagcgctcgc gctcggcctg
     1021 atcacttcgg cccccgatga catcgactgg cccgatgaaa ctcgcatcgc gatcgaggag
     1081 cgcgccagca tgtcgcccga cgcgctcacc ggcatggagg ccaacctgcg cttcgccggc
     1141 caggagacga tggagacgcg catcttcggc cgcctgacgg cgtggcagaa ctggatcttc
     1201 atccgcccga acgcggtcgg cgagggtggc gcgctgaagg tctacggcac cggcaccaag
     1261 ccgcagttcg acagaaatcg cgtctaacca aggactcgca tgagcaccgt caactacagc
     1321 gacaagatcc ccaacaacgt caacctgtcg gaagaccgca cgctgcagcg cgcgctcgaa
     1381 ggctggctgc cgaactacct cgactggtgg caggacatgg gcccgagcgg ctcgcagggc
     1441 ttcgacgtct acctgcgcac cgcgaccagc accacgcccg acggctgggc gaccttcggc
     1501 cacgtgaaga tgccggacta ccgctggggc atctttctcg cggcgcaaga gaacgagcgc
     1561 aagatcaact tcggcgagca caagggcgag gccgcctggc aggacgtgcc cggcgagcac
     1621 cgcgccaacc tgcgccgcat catcgtgacg cagggcgaca ccgagccggc atcggtcgag
     1681 cagcagcgcc acctgggcat caccgcgccc agccagtacg acctgcgcaa cctgttccag
     1741 gtcaatgtcg aagagggccg gcatctgtgg gcgatggtct acctgctgca caagcacttc
     1801 ggccgcgacg gccgcgaaga ggccgaggcg ctgctcgagc gccgttcggg cgacgccgac
     1861 aacccgcgca tcctcggcgc cttcaacgaa gagacgccgg actggctcgc gttcttcatg
     1921 ttcacctact tcaccgaccg cgacggcaag ttccagctca acgcgctggc cgagagcggc
     1981 ttcgacccgc tggcgcgcac gacgaagttc atgctgaccg aagaagcgca ccacatgttc
     2041 gtcggcgaga gcggcgtgtc gcgcgtgatc cagcgcacct gccaggtgat gaaggagacc
     2101 ggcatcgagg atcccgccga actgcgcaag aagggggtca tcgacctgcc gacgatccag
     2161 cgctacctga acttccacta cagcgtgacg atcgacctgt tcggcgccga ccagtcgagc
     2221 aacgcggcga tcttctacag cgccgggctg aagggccgct ttgaggagac caagcgcacg
     2281 gacgaccacc tgctgaaggg cgagggctac aagatcctcg aagtgaaaga cggcgtgctg
     2341 gtcgagaagg aagtgccgat gttgaacgcg ctgaacgagg tgctgcgcga cgacttcatc
     2401 aaggactcgc tcggcggcgt gaaccgctgg aacaaggtca tcgagaaggc cgggctgggc
     2461 ttccgactga ccttgccgca caaggccttc aaccgtcaca tcgggacctt cgccggcatc
     2521 cgcgtcagcc cggacggccg cgcgatcagc gaagccgaat gggccgcgaa tgtcggccag
     2581 tggctcgcga cggaagaaga ccgcgcgtat gtcgcctgcc tgatgggccg cgtcgtcgag
     2641 accggaaagt acgcgaactg gatcgcgccg ccggcgatcg gcatcaacaa gcagcccgtg
     2701 gacttcgagt acatccgctt caactgaagc ggcgcatgat tcgctgatga gcgtcgccga
     2761 aatcgtgctg gtcaagcagc acctgatcga ccccgaggtc tgcatccgct gcaacacctg
     2821 cgaagagacc tgcccggtcg atgcgatcac gcacgacgag cgcaactacg tcgtcaacgc
     2881 cgaggtctgc aacgcctgtg gtgcctgcct cggcccgtgc ccgaccggca gcatcgacca
     2941 ctggcgcgtc gtcgatcgca gcaaggtctt cacgctggcc gagcagttcg gctgggacga
     3001 gctgcccagc gtcgaggagg ccggcgcgac gacctccgac gactcgctcg cgaccctggt
     3061 cggcgccgaa gcgccggcgc aggtcaatgc cgcggtatcg ccgtcgtcga cgaaaccgcc
     3121 gtactcggcc gccgtgccgc agaccaacct gcacggcgtg aagaacccga tcaccgcgac
     3181 ggtcgcgggc aatctgcggc tcaccggcga aggcgcgagc ggcgacgtgc gccacatcgt
     3241 gctcgacttc ggctcgctgc ccttcccggt gctcgaaggc caggcgatcg gcatcatccc
     3301 gccgggtgtc gatgcgaacg gccgcgcgca ccacccgcgc atgtactcgg tcgcgagccc
     3361 gcgcagtggc gagcgcgagg gctacaacaa cctgtcgctg accgtgaagc gcatgaccga
     3421 aggcgacgac ggccagccct tccgcggcgt gtgctcgaac tacgtctgcg atctgaaaaa
     3481 gggcgacacc gtgcaggtca ccggcccgtt cggcgcgagc ttcctgatgc ccaaccatgc
     3541 cggctcgaac ctgctgatga tctgcaccgg caccggctcg gcgccgatgc gcgcgatgac
     3601 cgagcagcgc cgccgcctgc gcgccgtcgg ccagccgctg cccggcaaac tgatgctgtt
     3661 cttcggcgcg cgccgcgccg aagagctgcc gtacttcggc ccgctgacca agctgccggc
     3721 cgacttcatc gacacctgcc tggcgttctc gcgcgtgccc ggccagccgc gcacctacgt
     3781 gcaggaccgg ctgcgcgagc gcgcgagcga tgttgccgag ctgctgcgcg gcgacacctt
     3841 cgtctacctg tgcggcctga agggcatgga gaccggcgtg ctcgaggcgc tgtgcgacgc
     3901 gtgcacggcc gccggcatgg actggccggc gctgcaccgg cggttcgtcg atgagggccg
     3961 tttccacgtc gaaacgtact gaggcccgcg ggcacaatcc gggggctttg gggagcccgc
     4021 tcgatgactc gtcgcgaatg gaccatcggc ctgccgatcg tcgtgctgct gatgctgtgc
     4081 ctggtcgcct acctgcgcgg catggcgctg ggcaacgacg cgggcgcgct gaccagcctg
     4141 cgcgtgttcg gcccacccga cgcccagaag gtcgcggcgg tcatcggcag ccgtctgcat
     4201 gttctcgacg ccggtggcca gcgcctcgcc gatcaggaca tgcgcgcact cggcttcagc
     4261 gaagacccga acgacatgga ctggaccgtc gacgcgcaag gccgcaccga agcctggttc
     4321 ttcgaggaca cgccgccgcg cgtcgtgcgc tgcgcctgga gtgacgcgca gaccaggctc
     4381 gacaactgcg cgagcgcgat cgcgggtgcg aagctgaagg tcaacccggc ttcgcgcgcc
     4441 gtgcacctcg cggtcgaccg ccccggcgag cgcgtgttcg tgaccgacgc gaagggcggt
     4501 gcggtgcaga tgttcgattt cagcggcaag cggctcgcga agtccgacgc gggcgaggtc
     4561 gcgctcgatt tcccgaaccg gatccgctat ctcggcgaca acacgctgct ggtcggcgac
     4621 aacgaccacg gccgcgtggt ctggctcgct gccgagccgg gcaagccgat ccggctgctg
     4681 aagacgctgt acgcgcagga tcacggcggc gcgcgtctcg cccacacgaa ggtgaccgac
     4741 gcggcgatct cgccgagcgg cacgctgtgg atgctcgcga tgcgcttcgg ccaggccgac
     4801 ggcgacatcc tcgtcttcga tgccaatcac caaccggtgc gccgcgcggc gctgcgtggc
     4861 gatgccgatc cggtgatcgt cgagccgctc ggcgatgcgc tgctggtcgg cgatttctcg
     4921 ccgccgcggc tgtatcgcgt cgatgcgcaa gggcggctgc tcgacgactt cggcgacgcc
     4981 gggtttcgcg ccgacctgct gccgctgcag gcgcaggccg agcacgcgtc gctgtggatg
     5041 cgcgcggcgg cggtcggcgc cgtcgtgacc ttcgtggtcg gcagtctgct cgcgtggcgc
     5101 ttcagcgaga ggccggtgcc gccggggcgc atcgacgcgc cggcgctggc cgggatcgag
     5161 cgcgcgcgcg ccgcggcggc gcctttgcgg ttcccgatcg tcatcgagcg ctcggccgag
     5221 tacctcaggc aggagcgccg tctgttgtgg gccatggccg cggcgacggt ggcgctgttc
     5281 ggtggcgtgg cgtggatcct ggtcgcgaac ggcaagcaca tcgatctctc caaaatctcc
     5341 tggcggctct gggcgcaggc cgtcggcatg gccgcgctgg cgccgatcgc gatcgcatgg
     5401 acgtactggt tcggcggccg gcgttcgctg caagtgaccg aacacgcggt ccgctggcgc
     5461 agcggcgagc gcacggtcgc cgaggcgccg ttgcacgccg tgctcgcgtc ggccaatgcg
     5521 ctgttgatcg gcacgcgcat ggtgctgatt cgtcaacgca cgcgtgcccg caagcccggc
     5581 accccgatgt tcgacgcggc gctgatcgag cagagcatcc tcgcccggct gccgcgcgag
     5641 aacctggtcg acgatgcggc gctgacgcgt gaaatgctcc ggcgcaacgt ctacctgaag
     5701 gcgctgatcg tcgcggcgat cgtcggcaca atcctggtga tcgcgcactc gctctaccgg
     5761 gcgctcgggt gacgcgtcgc gcgcacaggt gactcgagtg cgtcgcgcac ggcaagcgcc
     5821 ttgcgtgcac aggccttgtg cgcaaggccg ggtcagggtc ctcgcgccgg tgcgtgcgcc
     5881 ccgagctggg gggcggcgcc cgcggattcg cactggcacg tggattgcat aggtcgttcg
     5941 ggaaggtcaa cggcgacctt cccgaacatc gatggatctg gccagcggcg gccgggtctg
     6001 cgcgcgccag cgcaaccgtc cggccggcgc ttgcccgccc gtccttcccg gaccgttttc
     6061 ggtccggccg cgccccggct ccagaaccca gcatctgcgc gtgcgcgccg cccttgggcc
     6121 gtgcggcgcg gccctgtgat ttgaacttcg ccttcctcag ctttcacgag accgccatga
     6181 aaccgaacac ctcttcgacc ccgcgcatcg acctcggccg ccgcagcctg atcaaggccg
     6241 ccgcggccac cggcgccgcc ggcctcgtgc cgggcctgca aagcgccgtc tacgccgccg
     6301 gctccgacaa gccggagaag gaggaagtgc ggatcggctt catcccgctg accgactgcg
     6361 catcggtcgt gatggcctcg gtgctgggct tcgacaagaa gtacggcgtg aagatcatcc
     6421 cgaccaagga ggcctcgtgg gccggcgtgc gtgacaagct cgtcaacggc gaactcgaca
     6481 tggcgcacgt gctctacggc ctgatctacg gcgtgcacat cggcgtcggt ggcccgaaaa
     6541 aggacatggc cgtgctgatg agcctgaaca acaacgggca ggccatcacc ttgtcgaaga
     6601 cgctggccga caagggcgct gtcgacggcc catcgctcgc caaggcgatg gccaaggaca
     6661 agcgcgaata cacctttgcc ggcacgttcc cgaccggcac gcatgcgatg tggatgcact
     6721 actggctggc agcctcgg
//
LOCUS       DATMJB010000668         2235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_860378, whole genome shotgun sequence.
ACCESSION   DATMJB010000668 DATMJB010000000
VERSION     DATMJB010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_860378"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /gene="ppsA"
                     /locus_tag="VNU48_14190"
     CDS             complement(<1..1944)
                     /gene="ppsA"
                     /locus_tag="VNU48_14190"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HWI12479.1"
                     /translation="MSSNPLATALVAPFEHLRMTDVESVGGKNASLGEMISQLAASGV
                     RVPGGFATTAHAFRQFLKHAGLDRRIEARLATLNTDDVRALAEAGAEIRHWIEATPFP
                     PELDAAIGAEFAKLTAGNPNASFAVRSSATAEDLPDASFAGQQETFLNVVGIAEVLAK
                     MKEVFASLYNDRAISYRVHKGFAHSDVALSAGVQRMVRSDMGAAGVMFTIDTESGFDQ
                     VVFITSSYGLGETVVQGAVNPDEFYVHKPALRAGKHAIIRRNLGSKLIKMEFATPEEK
                     AASGRLVKTVDTLHEQRNRYSLTDADVAELAGYALTIESHYGRAMDIEWGKDGVDGKL
                     YILQARPETVKSQQEGKLEQRYKLKGSGTVLAEGRAIGQKIGTGPVRIVHSIADMDQV
                     QAGDVLVTDMTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATEVL
                     TEGALVTVACSEGDTGYIYDGLLETEVSDVQRGELPACPVKITMNVGNPQLAFDFAQM
                     PNSGVGLARLEFIINNNIGVHPKAILDYPNIDGDLKKAVESVARGHASPRAFYVDKLT
                     EGIATIAAAFWPKPVIVRLSDFKSNEYRKLIGGSRYEPEEENPMLGFRGASRYVSEEF
                     GEAFAMECEALKHVRVEMGLTNVE"
     gene            1998..>2235
                     /locus_tag="VNU48_14195"
     CDS             1998..>2235
                     /locus_tag="VNU48_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12480.1"
                     /translation="MLEGARHRRRARTREPRRGSGAAGLGVHRGAGEKDAADFTVELP
                     IIVARPTLACLHHTMAQRTVFFISDGTGITAETFG"
BASE COUNT          319 a          780 c          731 g          405 t
ORIGIN      
        1 ctcgacattg gtcaggccca tctcgacccg cacgtgcttc agcgcttcgc attccatcgc
       61 gaaggcctcg ccgaattctt cgctgacata gcgcgatgcg ccgcggaagc cgagcatcgg
      121 gttctcttcc tccggctcgt agcgcgaccc gccgatcagc ttgcggtact cgttgctctt
      181 gaaatcggac agccgcacga tcaccggctt cggccagaac gccgcggcta tcgtcgcgat
      241 gccctcggtc agcttgtcga catagaacgc gcgcggcgac gcgtggccgc gcgccacgct
      301 ctcgaccgcc ttcttcaggt cgccatcgat gttcgggtag tcgaggatcg ccttcgggtg
      361 cacgccgatg ttgttgttga tgatgaactc gagccgcgcc aagccgacgc cgctgttggg
      421 catctgcgcg aagtcgaacg cgagctgcgg gttgcccacg ttcatcgtga tcttcaccgg
      481 gcaggcgggc agctcgccgc gctgcacgtc gctgacctcg gtctcgagca ggccgtcgta
      541 gatgtagccg gtgtcgcctt ccgagcaggc caccgtgacg agcgcgccct cggtcagcac
      601 ctcggtcgcg tcgccgcaac cgaccaccgc cggaatgccg agctcgcgcg cgatgatcgc
      661 cgcatgacac gtgcggccgc cgcggttcgt gacgatcgcg ctcgcgcgtt tcatcaccgg
      721 ctcccagttc gggtcggtca tgtcggtgac cagcacgtcg ccggcctgca cctggtccat
      781 gtcggcgatg ctgtggacga tgcgcaccgg cccggtgccg atcttctggc cgatcgcgcg
      841 gccctcggcg agcaccgtgc cgctgccctt gagcttgtag cgctgctcca gtttgccctc
      901 ctgctggctc ttcaccgtct cgggccgggc ctgcaggatg tagagtttgc cgtcgacgcc
      961 gtccttgccc cattcgatgt ccatcgcgcg gccgtagtgc gactcgatcg tcagcgcgta
     1021 gccggcgagt tcggccacgt cggcgtcggt gagcgaatag cggttgcgct gctcgtgcag
     1081 cgtgtcgacg gtcttcacca ggcgcccgct cgcggccttc tcctcgggcg tggcgaactc
     1141 catcttgatc agtttcgagc cgaggttgcg ccggatgatc gcgtgcttgc cggcacgcag
     1201 cgcaggcttg tgcacataga actcgtccgg gttcacggcg ccctgcacca cggtctcgcc
     1261 cagcccgtag ctcgaggtga tgaacacgac ctgatcgaac cccgattcgg tgtcgatcgt
     1321 gaacatcacg ccggccgcgc ccatgtccga tcgcaccatg cgctgcacgc cggccgacag
     1381 cgccacgtcg ctgtgcgcga agcccttgtg gacgcggtag ctgatcgcac ggtcgttgta
     1441 caggctcgcg aacacctcct tcatcttcgc gagcacctcg gctatgccga cgacgttcag
     1501 gaaggtctcc tgctggccgg cgaacgaagc gtcgggcagg tcctcggccg tcgcggacga
     1561 gcgcaccgcg aacgacgcgt tcgggttgcc ggcggtcagc ttcgcgaact cggcgccgat
     1621 cgctgcgtcg agttcgggcg ggaacggcgt cgcctcgatc cagtggcgga tctcggcacc
     1681 ggcctcggcc agcgcgcgca catcgtcggt gttcagggtt gccaggcgcg cctcgatgcg
     1741 ccgatccagc cccgcgtgct tcaggaactg ccggaacgca tgcgccgtgg tggcgaagcc
     1801 gcccggcacg cgcacgcccg aggcggccag ttggctgatc atttcgccga gcgaggcgtt
     1861 cttgccgccc accgactcga cgtcggtcat cctcaggtgt tcaaacgggg cgaccagggc
     1921 ggtcgccagg gggttgcttg acatgacaaa gctccgggat ggtgaaaaac gggtgcttca
     1981 gggcccgggc ccgagccgtg ctcgagggcg ctcgccaccg gcgccgggcg cgcacgcgcg
     2041 aaccccggcg agggtcgggt gcagcgggcc ttggcgttca tcggggggcg ggcgagaagg
     2101 atgcggccga ttttacggtc gaactgccta tcatcgtcgc caggcccact cttgcctgcc
     2161 tgcaccacac catggcccaa cgcaccgtgt tcttcatctc cgacggcacc ggcatcaccg
     2221 ccgagacctt cggca
//
LOCUS       DATMJB010000669         2236 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_90548, whole genome shotgun sequence.
ACCESSION   DATMJB010000669 DATMJB010000000
VERSION     DATMJB010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90548"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..881)
                     /locus_tag="VNU48_14200"
     CDS             complement(<1..881)
                     /locus_tag="VNU48_14200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWI12481.1"
                     /translation="MPSTTPAPILNVPSPAAQKLELLRELFPQAVETAPDGSVRVNAA
                     AIQQALDPANPAGIRLEEDGYELRWVGKREAYHSAFVPPAKIVQPCPGDSADWDATSN
                     LVIKGDNLDALRLLRQSYFGQVKLIYIDPPYNTQSDAFVYRDDFTVRQSEVLSALGYS
                     ADNIDYIKNIYGARTHSGWLSFMYPRLLLAKDLLRDDGVIFISIDDNEQAQLKLLCDE
                     VFGQENFISSLTWEKGRKNDAKFFSNGHEYMLVYAKSQAYLRENQTLWREEKPGAREI
                     WDKYLELREQHGADNAL"
     gene            complement(1562..2050)
                     /locus_tag="VNU48_14205"
     CDS             complement(1562..2050)
                     /locus_tag="VNU48_14205"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWI12482.1"
                     /translation="MNDPTTLPPVEGGCLCGALRYRATPTHREGYYCHCRMCQLAFGN
                     TRAAFVNLRKDEVVWTQGTPSHFASSAFARRGFCGRCGTPLSFEYLDSQRMDLSAGSL
                     DDPSAFRPTAHFAVESRIAAWHADDGLPGERIDANAQIAARWKQAYGDDVVPDVAATR
                     AP"
BASE COUNT          380 a          666 c          762 g          428 t
ORIGIN      
        1 atcagcgcgt tgtctgcgcc gtgctgctct cgcaattcca ggtacttgtc ccagatttcc
       61 cgagcgcccg gcttctcctc gcgccagaga gtctggtttt cgcggagata agcctgtgac
      121 ttggcataga ccagcatgta ctcgtggccg ttggagaaaa acttggcgtc attctttcgg
      181 cctttttccc aggtcaggct tgagatgaag ttctcctgcc caaacacctc atcacacaac
      241 aacttcaact gcgcctgctc gttgtcgtca atcgaaatga aaatcacgcc gtcgtcgcgc
      301 agcaggtctt tggccagcag caggcgcggg tacatgaagc tgagccagcc gctgtgcgtg
      361 cgagcgccgt agatgttctt gatgtagtcg atgttgtcgg cgctgtagcc taaggcgctg
      421 agcacctcac tctggcgcac tgtgaagtcg tcgcgataga cgaatgcatc gctttgggtg
      481 ttgtagggcg ggtcgatgta gatgagcttg acctggccaa agtagctttg gcgtagcagg
      541 cgcagggcgt cgaggttgtc gcccttgatg acgaggttgc tggtggcgtc ccagtcggcg
      601 ctgtcgcccg ggcagggctg cacgattttg gcgggcggca caaaggcgct gtggtaggcc
      661 tcgcgcttgc ccacccagcg cagttcgtag ccgtcttctt ccaagcgaat gccggccggg
      721 ttggccgggt ccagcgcttg ctggatggcg gcggcattga cgcgcacgct gccgtcgggc
      781 gcggtttcca cggcttgtgg gaacagctcg cgcagcagtt caagcttttg cgcggcggga
      841 ctcgggacgt tgaggatggg ggccggggtg gttgagggca tggcggcgag ttgtgcggaa
      901 tcagtcgaat gagatggcga tggcgatggc gatatcggag gatagtgttt tcaaacgccc
      961 tttttccttt cccgtggcac cccgaacacg gcagattcgg ggcagaacac agcgcccgcc
     1021 gcgcttgatt ctggctttgg atgcagttcg ggtgccattg aacggtgttc aaagcctgcg
     1081 gcgggcagcg gagactctga ggcgcagcgg ccggttcggc ctgaacacgt ttggcttggc
     1141 cgcgtgtagg gacgaatcac agatcctacg cgggtcggtc tgtccctgtt tccgtcactt
     1201 cgaagcaaag tcgttgccgc gaaatcgcgg cgatgtcctt gcgctcgcca agcagccgga
     1261 acacgtccag gtgctctccg agctcgaact aggacgaaag tagcgggaac ttggccaccc
     1321 gccaagtgcg aagacctgga atgcctagac ttgggccaca aaggcaattg acggcgaaca
     1381 acgacctcgg cggactgcga cctgcaccga caactgggca gtgtgcgagc tgatctatca
     1441 gcgcgacaga tcgcccgctc gataccggtc gatcggctac gtcagacaat ggccgcatgc
     1501 agtcgaccgt ggacgacgac gccatcgatg tatcgccggt tcgtcctgtt ccagtgcgcc
     1561 ctcaaggcgc acgcgtcgcc gcgacgtcgg gcaccacgtc gtcgccgtag gcctgcttcc
     1621 agcgtgccgc gatctgggcg ttggcgtcga tgcgctcgcc gggcaggccg tcgtcggcgt
     1681 gccatgcggc gatgcgcgat tcgaccgcga agtgcgcggt ggggcggaac gcggacgggt
     1741 cgtccagact gccggccgac aggtccatgc gctgcgagtc gaggtactcg aagctcagcg
     1801 gcgtgccgca gcggccgcag aagccgcgcc gcgcgaaggc cgacgacgcg aaatggctcg
     1861 gcgtgccctg cgtccacacg acctcgtcct tgcgcaggtt gacgaaggcg gcgcgcgtgt
     1921 tgccgaaggc gagctggcac atccggcagt gacagtagta gccctcgcgg tgcgtcggcg
     1981 tggcgcggta gcgcagcgcg ccgcacaggc agccgccttc aaccggcggc agggtcgtgg
     2041 ggtcgttcat aggcaatgtc ccgttcggtt tcagtaccgg cacgaccgtg acgccgctag
     2101 aaccggctcg catcggaggc gcgcagcgcg ccgatgcgga acagtctgac agtcggcggc
     2161 gcttcgttca agcgccgttg caatacctca ccgcagaggc cgcggcgcgg tcaggccgcg
     2221 acggtgtagt tcaggg
//
LOCUS       DATMJB010000670         2128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_505671, whole genome shotgun sequence.
ACCESSION   DATMJB010000670 DATMJB010000000
VERSION     DATMJB010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505671"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VNU48_14210"
     CDS             complement(<1..914)
                     /locus_tag="VNU48_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HWI12483.1"
                     /translation="MTDLKTPAITDVRVIPVAGRDSMLLNLSGAHAPFFTRNLVIVTD
                     SAGRTGVGEVPGGEKIRQTLEDTGALIVGQAIGTMQQLLGVVRARFADRDAGGRGLQT
                     FDLRTTIHVVTAIESALLDLLGQHLGRPVAALLGEGQQREAVAVLGYLFFVGDRSRTD
                     LPYASEPDADDAWCRLRHESAMTPEAIVRLAEAAHARYGFEDFKLKGGVLAGETEVEA
                     IRALHERFPAARVTLDPNGGWGLQDAVRLTRDLHGVLAYAEDPCGAEDGFSGREVMAE
                     FRRATGLPTATNMVATDWRQLVHSLSLQS"
     gene            complement(971..1705)
                     /locus_tag="VNU48_14215"
     CDS             complement(971..1705)
                     /locus_tag="VNU48_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWI12484.1"
                     /translation="MNPSPPRRHTRTLAHRIVESLGERIRDGALAPGDRLPTEAAFMA
                     EFGVSRTVVREAISRLQAAGLVETRHGVGTFVVGLGDTSAFRIAPEQLATLRDVVAML
                     ELRIGVETEAAGLAAQRRSAANLVVMRDALDAFTQAVEAGRDAVGADFRFHLEITKAT
                     QNLHFSNLLATLGTMIIPRARLDPARPPDEERLAYLRRVNAEHLSILDAIENQDVEAA
                     RAAMRTHLANSRERRRRAAAATREKP"
     gene            1864..>2128
                     /locus_tag="VNU48_14220"
     CDS             1864..>2128
                     /locus_tag="VNU48_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWI12485.1"
                     /translation="MPPAPVRCATFARLLLTGAAGGLGRVLRPRLKAHCAVLRLSDIA
                     PLGAAGDGEEIVFAALQDGAAVHALLDGVDAVVHLGGVSVEGPF"
BASE COUNT          295 a          730 c          768 g          335 t
ORIGIN      
        1 gactgcagcg acagcgaatg gaccagctgg cgccagtcgg tggcgaccat gttggtggcg
       61 gtcggcagtc cggtcgcgcg gcggaattcg gccatcacct cgcggcccga gaagccgtcc
      121 tccgcgccgc acgggtcttc ggcgtaggcg agcacgccgt gcaggtcgcg cgtcaagcgg
      181 accgcgtctt gcaagcccca gccgccgttc gggtcgagcg tgacgcgcgc ggccgggaag
      241 cgttcgtgca gcgcgcggat cgcctcgacc tcggtctcgc cggcgagcac gccgcccttc
      301 agcttgaagt cctcgaagcc gtagcgcgcg tgcgcggcct cggccaggcg cacgatcgcc
      361 tcgggcgtca tcgcgctctc gtggcgcaac cggcaccagg cgtcgtcggc gtcgggctcg
      421 cttgcgtacg gcaggtcggt cctgctccgg tcgccgacga agaacagata gcccagcacc
      481 gcgaccgcct cgcgttgctg gccttcgccg agcagcgcgg ccaccggccg cccgaggtgc
      541 tggccgagca ggtccagcag cgccgactcg atcgcggtga ccacatggat ggtcgtgcgc
      601 aggtcgaagg tctgcaggcc gcggccgccg gcgtcgcggt cggcgaagcg cgcgcgcacc
      661 acgccgagca gctgctgcat cgtgccgatc gcttggccga cgatcagcgc gcccgtgtct
      721 tcgagggtct ggcggatctt ctcgccaccg ggcacttcgc cgacgccggt gcggccggcg
      781 ctgtcggtga cgatcaccag gttgcgcgtg aagaacggcg cgtgcgcgcc gctcagattc
      841 agcagcatcg aatcgcggcc cgcgaccggg atcacgcgca catcggtgat tgcgggcgtc
      901 ttgaggtcag tcatcgtaca actatcctag cccagatgca gcgcccccga caaggcccgg
      961 cggtcgccga tcagggtttc tcgcgcgtcg ccgcggcggc acggcgccgc cgttcgcggc
     1021 tgttggccaa gtgggtgcgc atcgccgcgc gggccgcctc gacatcctga ttctcgatcg
     1081 catcgaggat gctgaggtgc tcggcgttga cgcgtcgcaa gtaggcgagc cgctcctcat
     1141 cgggcggccg cgcgggatcg agtcgcgccc gcgggatgat catcgtgccc agcgtggcca
     1201 acaagttgga gaagtggagg ttctgggtcg ccttggtaat ttccaggtga aaccggaagt
     1261 cagcgcccac tgcgtctctg ccggcttcga cggcttgcgt gaaggcatcc aaggcgtcgc
     1321 gcatcacgac caggttcgcg gccgaacggc gttgcgcggc cagacctgcg gcctcggtct
     1381 cgacgccgat ccgcagctcc agcatcgcga ccacgtcgcg cagggtggcg agttgttccg
     1441 gtgcgatgcg gaacgccgac gtgtcgccca gcccgaccac gaaggtgccg acgccgtggc
     1501 gcgtttcgac gagccccgcg gcctggagcc gcgagatcgc ctcgcggacg accgtgcggc
     1561 tgacgccgaa ttcggccatg aacgcggcct ctgtcggcag cctgtctccg ggggccaggg
     1621 cgccgtcgcg aatgcgctcg cccagtgact cgacgattcg gtgagccagc gtgcgggtgt
     1681 ggcggcgggg aggcgagggg ttcatcgacc tttggtacaa accccgatgc atcgctcttg
     1741 acgctccgga gtggttcacg gaaactgggc ttgttgtacg acaactgatg atattgtcgc
     1801 tcaggatgcc gcgccaagcg aggtcgcacc atccgcgcaa accccctcga ggcgccccgc
     1861 agcatgccgc cagctcccgt ccgttgcgcg actttcgcgc gcctgctgct gaccggtgcg
     1921 gccggcggtc tgggccgggt gctcaggccg cgactgaaag cacactgcgc ggtgctgcgg
     1981 ctcagcgaca tcgcgccgct cggcgcggcc ggcgatggcg aggagatcgt cttcgcggcc
     2041 ctgcaggacg gcgcggcggt gcacgcgctg ctcgatggtg tggacgcggt ggtgcacctg
     2101 ggcggcgtgt cggtcgaggg gcccttcg
//
LOCUS       DATMJB010000671         5025 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_589554, whole genome shotgun sequence.
ACCESSION   DATMJB010000671 DATMJB010000000
VERSION     DATMJB010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589554"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VNU48_14225"
     CDS             <1..398
                     /locus_tag="VNU48_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWI12486.1"
                     /translation="LLTIRGLLAACKQREVDERIVLDDAAVVAIIDKLVKQRKDSIAQ
                     FSAGNRPDLVDKETAELHVLEAYLPQRLGADAVATEVAAVVAALGTELGRAAGPGDMG
                     KVMGAIKARLAGKADMALVSAAVKKALAP"
     gene            395..739
                     /locus_tag="VNU48_14230"
     CDS             395..739
                     /locus_tag="VNU48_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HWI12487.1"
                     /translation="MNAPSVQQLSADVCVAPQLDPAAMAWAAQAGFKSVVNNRPDFEG
                     GPDQPTSAAIEAAAQTAGLRYAYLPVHPAVQTPDEIARFAALLAELPKPILAFCRTGT
                     RSGKLYRAATGG"
     gene            908..1501
                     /locus_tag="VNU48_14235"
     CDS             908..1501
                     /locus_tag="VNU48_14235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HWI12488.1"
                     /translation="MSFLLQVSRWIDRLSEFVGRCVAWLILAAVLISAVNAVVRKLFN
                     MSSNGFLEIQWYLFAAVFLLAAGYTMLRQEHVRIDVILGRFSKRTQIKIEAVCIVVFL
                     FPFCINVITLVWPLVVKAYLTGEMSSNAGGLIRWPVFAMVPAGFALLLIQGVSELIKR
                     IAFLQGLIDDPTRKKQAKSAEEELADSIRATTAGAAR"
     gene            1498..3231
                     /locus_tag="VNU48_14240"
     CDS             1498..3231
                     /locus_tag="VNU48_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HWI12489.1"
                     /translation="MIEFLSAYMAPIMFGALILFLLIGYSVAFSLAACGLLFGLIGVE
                     LGLMPATLLQALPLRIFGIMQNDTLLAIPFFTFMGLILERSGMAEDLLDTIGQLFGPV
                     RGGLAYAVILVGAMLAATTGVVAASVIAMGLISLPIMLRTGYDRRVASGVIAASGTLA
                     QIIPPSLVLIIMADQLGQSVGDLYKGAFVPGFVLTGFYIGYVLLVSLVKPSYVPALPA
                     AARTIREDNGSPGLRSLGVLFVASTALAILFAKTRPASTPTDELIVVSMCVGVGTAFV
                     LAVINKTLKLGLLSRMAERVTFVLIPPLGLIFLVLGTIFLGVATPTEGGAMGATGALA
                     MALGRKRIDLRLLRQAMDSTMKLSCFVVFILVGSTVFSLSFQAVDGSRWVEHLLGGLP
                     GGALGFLIVVNILIFVLAFFLDFFELSFIVVPLIGPVAIKLGIDPVWFGVLLAVNMQT
                     SFMHPPFGFALFYLRSVAPADDYQDRVSGKTIAKITTGQIYWGAIPFVIIQIVMVGLI
                     IAFPGIVTRDTNKAAEGNLDQLQQQIDNGLGATQKPAPTLPGADEKGKDAPEDPMEAI
                     RRANEQDKAKK"
     gene            complement(3314..4396)
                     /locus_tag="VNU48_14245"
     CDS             complement(3314..4396)
                     /locus_tag="VNU48_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI12490.1"
                     /translation="MERRSFVRSAGIAGVLAAGAAPAIVHAQAAVRWRMASSFPKAAD
                     IIYGGADVFAKRMSDMTGGKFQISVHGPGELMPAFGVVDGVQAATVEMAHTAPYYFFG
                     KDPTFALGCAIPFGLNSRQMTAWMYEGNGLKLMREFYAKYNIINFPGGNTGAQMGGFF
                     RKPIQSLADIKGLKFRIGGFGGKVIERIGGVPQNIPGGEIYQALEKGTIDAAEWIGPY
                     DDEKLGLNKIAPNYGYPAWWEGGPQLDFFINDKAYNALSAEYKAMIEVAASHAHVDMQ
                     AKYDSRNAAALKRLVGSGTKLFRFPKDFMEASFKEATALYSELSASNPGWKKIYEDFS
                     TFRRDSNLWFRFTEAGFDDFMQAQKL"
     gene            complement(4499..>5025)
                     /locus_tag="VNU48_14250"
     CDS             complement(4499..>5025)
                     /locus_tag="VNU48_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWI12491.1"
                     /translation="GTTVLPRHIDIHEDPHLLRGKGSAPFDDEGVLTKPRDVLKAGEV
                     QGYFLSTYSGRKLGMRTTGHAGGSQNLTLTSRLTQPGDDLPAMLKKLGRGLFVIELMG
                     QGVNYVTGDYSRGAAGFWVEHGRLQYPVHEITIAGNLRTMFKGIVAVGADAYTMGSKT
                     IGSVLIDRLKIAGL"
BASE COUNT          759 a         1718 c         1652 g          896 t
ORIGIN      
        1 gcctgctgac gatccgcggc ctgctcgcgg cctgcaagca gcgcgaggtc gatgagcgca
       61 tcgtgctcga cgacgccgcc gtcgtcgcga tcatcgacaa gctggtcaag cagcgcaagg
      121 actcgatcgc ccagttcagc gcgggcaatc ggccggatct ggtcgacaag gaaaccgccg
      181 agttgcatgt gctcgaggcc tacctgccgc agcgtctggg cgccgatgcg gtcgccaccg
      241 aagtcgccgc cgtcgtcgct gcgctgggca ccgaactcgg ccgggccgcg gggccgggcg
      301 acatgggcaa ggtcatgggc gcgatcaagg cacgcctggc cggcaaggcc gacatggcac
      361 tggtctcggc ggccgtcaag aaggcgctgg cgccatgaac gcaccgtcgg tccagcagct
      421 cagcgcagac gtctgcgtcg ccccgcaact cgaccccgcg gcgatggcct gggccgcgca
      481 ggccggcttc aagagcgtcg tgaacaaccg gcccgacttc gagggcggcc cggaccagcc
      541 gaccagcgcc gcgatcgaag ccgcagcgca gaccgccggc ctgcgctacg cgtacctgcc
      601 ggttcatcca gccgttcaga cgcccgacga gatcgcgcgc ttcgccgcgc tgctcgccga
      661 actgccgaag ccgatcctgg cgttctgccg caccggcacg cgctcgggca agctctaccg
      721 cgccgccacc ggcggctgat ccgggcgcgc tccgcgagcc cgggccgcgc cgcgccgcgc
      781 cacgccgcgc gctcagggtt acaccgcgca gcgatcgcgg gacacgcccc tagaatcgcg
      841 cgggccatga cgggcggccg cgcccgtgcg acgctcctgc actcggcagc ttttttgggg
      901 agacaccgtg tcgttcttgc ttcaggtttc gcgctggatc gaccggctca gcgagttcgt
      961 cgggcgctgc gtcgcgtggt tgattctggc cgcggtgctc atcagtgcgg tgaacgcggt
     1021 cgtgcgcaag ttgttcaaca tgagctccaa cgggttcctg gaaatccagt ggtatctgtt
     1081 cgctgctgtc ttcctgctcg ccgccggcta caccatgctg cgccaggagc atgtgcgcat
     1141 cgacgtgatc ctgggccgtt tcagcaaacg cacgcagatc aagatcgaag ccgtctgcat
     1201 cgtggtcttc ctgtttccgt tctgcatcaa cgtcatcacc ctggtctggc cgctggtggt
     1261 caaggcctac ctgacgggtg agatgtcgtc caacgccggc ggcctgatcc gctggccggt
     1321 gttcgcgatg gtccctgcgg gcttcgcgct gctgctgatc cagggcgtgt cggagctgat
     1381 caagcgcatc gccttcctgc agggcctgat cgacgacccg accaggaaga agcaggccaa
     1441 gtcggccgag gaagagctcg ccgattcgat ccgcgccacg accgccgggg cggcgcgatg
     1501 atcgagttcc tgtcggccta catggccccg atcatgttcg gggccctgat tctcttcctg
     1561 ctgatcgggt actcggtggc cttctcgctg gctgcctgcg gcctgctgtt cggcctgatc
     1621 ggtgtcgagc tcggcctgat gccggcgacg ctgctgcagg ccctgccgct gcgcatcttc
     1681 ggcatcatgc agaacgacac gctgctggcg attccgttct tcactttcat gggcctgatc
     1741 ctcgaacgat cgggcatggc cgaagacctg ctggacacca tcggccagtt gttcggcccg
     1801 gtgcggggcg gcctggccta cgcggtgatc ctggtgggtg cgatgctcgc ggccaccacg
     1861 ggcgtggtcg cggcctcggt catcgcgatg ggcctgatct cgttgccgat catgctgcga
     1921 acgggctacg accggcgcgt ggcgtccggc gtgattgccg cgtccggcac gctggcgcag
     1981 atcatcccgc cgtccctggt gctgatcatc atggccgacc agctcggcca gagcgtcggc
     2041 gacctgtaca agggcgcgtt cgtccccggc ttcgtgctga ccggcttcta catcggctac
     2101 gtgctgctcg tgagcctcgt caagccgagc tacgtccccg cgttgccggc cgcggcgcgc
     2161 acgatccgcg aagacaacgg ctcgcccggc ctgcgctcgc tgggcgtgtt gttcgtggcg
     2221 tccaccgcgc tggcgatcct gttcgccaag acgcgcccgg cctcgacccc gaccgacgag
     2281 ctgatcgtgg tgtcgatgtg cgtcggcgtc ggcacggcct tcgtgctggc ggtcatcaac
     2341 aagacgctga agctcggcct gctgtcgcgg atggccgagc gcgtcacctt cgtcctgatc
     2401 ccgccgctgg ggctgatctt cctggtgctg ggcacgatct tcctcggcgt cgccacaccc
     2461 accgagggcg gtgcgatggg cgccacgggc gcactggcga tggcgctggg gcgcaagcgc
     2521 atcgacctga ggctgctgcg ccaggccatg gactcgacga tgaagctgtc gtgcttcgtc
     2581 gtcttcatac tggtcggctc cacggtcttc agcctgtcgt tccaggccgt cgacggctcc
     2641 agatgggtgg agcatctgct cggtggcctg cccggcggcg cgctcggctt cctgatcgtg
     2701 gtcaacatcc tgatcttcgt gctggcgttc ttcctcgact tcttcgagct cagcttcatc
     2761 gtcgtgccgc tgatcgggcc ggtggccatc aagctgggca tcgacccggt ctggttcggg
     2821 gtgctgctgg cggtgaacat gcagacctcg ttcatgcacc cgccgttcgg cttcgcactg
     2881 ttctacctgc gcagcgtcgc ccccgccgac gactaccagg accgcgtcag cggcaagacc
     2941 atcgccaaga tcacgaccgg gcagatctac tggggcgcga tcccgttcgt gatcatccag
     3001 atcgtgatgg tcggcctgat catcgccttc ccgggcatcg tcacgcgcga cacgaacaag
     3061 gccgccgaag gcaacctcga ccagctgcag cagcagatcg acaacggcct gggagccacg
     3121 cagaaacccg cccccacact gccgggtgcc gacgagaaag gcaaggacgc gcccgaagac
     3181 ccgatggagg cgattcgccg ggccaacgag caggacaagg cgaagaagta gctgcacccg
     3241 cccccgccgc gcacgccaca cgcgcacgca gaaaggggcc cagagggccc ctattccttg
     3301 tgcaactcgg cgctcagagt ttctgcgcct gcatgaagtc gtcgaagccg gcttcggtga
     3361 agcggaacca gagattggaa tcgcgccgga aggtcgagaa gtcctcgtag atcttcttcc
     3421 accccgggtt gctggccgac agttcgctgt acagcgccgt cgcttccttg aacgacgcct
     3481 ccatgaagtc cttggggaag cggaacagct tggtcccgct gccgacgagc cgcttgagcg
     3541 cggcggcgtt gcgcgagtcg tacttggcct gcatgtcgac gtgggcgtgc gacgccgcca
     3601 cctcgatcat cgccttgtac tcggccgaca gcgcgttgta ggccttgtcg ttgatgaaga
     3661 agtcgagctg cgggccacct tcccaccacg ccgggtaacc gtagttgggg gcgatcttgt
     3721 tcaggccgag cttctcgtcg tcgtaggggc cgatccattc ggccgcgtcg atggtgccct
     3781 tctccagcgc ctggtagatc tcgccgccgg gaatgttctg cggcacgccg ccgatgcgct
     3841 cgatcacctt gccgccgaag ccgccgatgc ggaacttgag gcccttgatg tcggccagcg
     3901 actggatcgg cttgcggaag aagccgccca tctgcgcacc cgtgttgccg ccggggaagt
     3961 tgatgatgtt gtacttggcg tagaactcgc gcatcagctt caagccgttg ccttcgtaca
     4021 tccaggcggt catctgccgc gagttcaggc cgaacgggat cgcgcagccg agcgcgaagg
     4081 tcgggtcctt gccgaagaag tagtacggcg cggtgtgcgc catctcgacg gtggcggcct
     4141 gaacgccgtc gacgacgccg aaggccggca tcagctcgcc gggaccgtgg acgctgatct
     4201 ggaacttgcc gccggtcatg tcgctcatcc gcttggcgaa gacgtcggcg ccaccataga
     4261 tgatgtctgc ggctttcggg aaactcgacg ccatgcgcca gcgcacggcc gcctgcgcgt
     4321 gcacgatggc cggagcagcg cctgcggcca gcacgcccgc gatacccgca ctgcggacga
     4381 aggaacgacg ttccatgaat ggatctcctg aagactcggg cgtgtgcccg ggggaatgaa
     4441 caggccggat tctaggagtc ggacccccgc ctgccgcagc gggtttcccc cgtcggcctc
     4501 acagaccggc gatcttcagc cggtcgatca gcaccgagcc gatcgtcttg ctgcccatcg
     4561 tgtaggcatc ggcgccgacc gcgacgatcc ctttgaacat cgtgcgcaga ttgcccgcga
     4621 tggtgatctc gtgcaccggg tactgcaggc gcccgtgctc gacccagaag ccggccgcgc
     4681 cgcgcgagta gtcgccggtc acgtagttca cgccctgccc catcagctcg atcacgaaca
     4741 ggcctcggcc cagcttcttc agcatcgccg gcaggtcgtc gccgggctgc gtcagccggc
     4801 tcgtcagcgt caggttctgc gaaccgccgg cgtggccggt cgtgcgcatg ccgagcttgc
     4861 ggcccgaata cgtcgacaga aaatagccct gcacctcacc ggccttcagc acgtcgcgcg
     4921 gcttcgtcag cacgccttcg tcgtcgaacg gggcgctgcc cttgccgcgc aggaggtgcg
     4981 ggtcttcatg gatgtcgatg tgccggggca gcacggtggt gccca
//
LOCUS       DATMJB010000672         4942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_583340, whole genome shotgun sequence.
ACCESSION   DATMJB010000672 DATMJB010000000
VERSION     DATMJB010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583340"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VNU48_14255"
     CDS             <1..326
                     /locus_tag="VNU48_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HWI12492.1"
                     /translation="PMYAFMDAEYNHFEVESENMGDAIQYLEDGMPVEVVFYDGKAIS
                     VELPTSLVREITDTEPAVKGDTSGKVLKSAKIATGFEISVPLFVQTGDKVEIDTRTHE
                     YRKRV"
     gene            396..1325
                     /locus_tag="VNU48_14260"
     CDS             396..1325
                     /locus_tag="VNU48_14260"
                     /inference="COORDINATES: protein motif:HMM:NF023354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent oxidative N-demethylase subunit
                     alpha family protein"
                     /protein_id="HWI12493.1"
                     /translation="MSFDFAAVRAPFRMQPGLRRLAAGSAPLTPNLPGAPALVEKTIV
                     LRDRAGEALVTAPGFDAGPALQTLMQQAVLDQPHCFDWDGAHGFEARLLGWSVRGAEV
                     AGDGPRELGELLRALPAAWRLPGLLSLALAEDFAVIDGASARIPWLAVCLPSRWAPAD
                     KVGRHFVEVHAPVADNALLLGAADHLAQLVTGTERWERFVWSISSDARLAQHPKHGAP
                     PGWPAALDAEALAGAAHFRSEHQTFIPIASVRQAVFTILVESRPLTEVIDSPERARQL
                     RDALASMSDAVLAYRGLVDARERLLAWLAARSA"
     gene            1322..3253
                     /gene="mnmC"
                     /locus_tag="VNU48_14265"
     CDS             1322..3253
                     /gene="mnmC"
                     /locus_tag="VNU48_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HWI12494.1"
                     /translation="MNSAPVVPGTLAFDDHGVPRSPLYGDIYHPRSGAPAQARHVFLG
                     GNELPRRWQGRDRFVVLETGFGLGHNFLATWQAWRDDPQRCERLVFIAIEQHPLTLDD
                     LRSVHRHSALPELADALQRAWPPLTHNLHRLGFDGDAVELLLAFGDVQVWLPELVAEV
                     DAFYLDGFAPALNPRMWDERVCKALGRLATPGATLATWSAAHALRRHLTSAGFEVRLG
                     AGQGGKRDITLARYAPGFTPRRTPARQGRGADADDRHALIVGAGLAGCAAACALARQG
                     WRSTVLERRRAPASEASGNPAGLFHGIVNAQDGVHARFNRAAALAAHAAVSAALAHGV
                     SGGTTGLLRLETTLDAVGMHALLQRLGLPPDYVQALDASAASARAGLALAHPAWFYPA
                     GGWVDPAGLARWFLARAANRVELRADVHVHSLRRIDSGWALLDAAGRTIAQACTVVLA
                     NAADALRLLGAPDWPIESVRGQISIASATRLATPRLPVAGAGYLLPASAGRAMFGATA
                     QRGDADDSVRAADHVFNLAQLARLTGRPTGLRVDELTGRTAWRCSATDRLPLIGAVPD
                     DNAAASARLDQPRFVPRLPGLFMFSALGSRGITWSALGAQLLASVVTGAPAPVEASLL
                     DAVDPARFVSRRARLASAG"
     gene            complement(3219..4334)
                     /locus_tag="VNU48_14270"
     CDS             complement(3219..4334)
                     /locus_tag="VNU48_14270"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12495.1"
                     /translation="MHQAIAGKTFDGLLQLPGLAMPTRIVRGVHDAITQGVYAAVRHG
                     GAATLALAAGVERHADDPTRALGHKEQALRGALSGAFGDTLAASGSALAVQMGLHGRR
                     EPLTPEALASLRPRVCVFLHGLACDEQSWRLVTDAWAASPWASTLHASEPIDYGALLE
                     HELGVSAVWLRYNSGLAIEGNAQQLADLMVQVAQGTPQVHEWLLIGHSMGGLVARRAH
                     ALATAQGLGWARRVPMIVCLGSPHLGASLAQAGHFAAAALNVSEVTPPLGRIVDARSR
                     GIKDLRRGLRSAPRAPDGPALRLVFGTLGDEAGTGLGPLVGSLLGDGLVHAGSAADDA
                     ASGDVQRVELAGLGHMSLLNHPRVYAVIRRWLGAPDG"
     gene            complement(4471..4656)
                     /locus_tag="VNU48_14275"
     CDS             complement(4471..4656)
                     /locus_tag="VNU48_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12496.1"
                     /translation="MAKPNYSFEKRQRELAKKKKKEEKLKEKAGRKVGEPGGDDAPAD
                     DDTVSGAPPADAPVPGP"
     gene            complement(4759..>4942)
                     /locus_tag="VNU48_14280"
     CDS             complement(4759..>4942)
                     /locus_tag="VNU48_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase A"
                     /protein_id="HWI12497.1"
                     /translation="VVTDNGGQIIDVRGLSIADPAALESEINQWPGVVGVGIFARHKA
                     DVCLLGTAQGVRTLTF"
BASE COUNT          629 a         1836 c         1775 g          702 t
ORIGIN      
        1 acccgatgta cgcgttcatg gacgccgaat acaaccattt cgaggtcgag tccgagaaca
       61 tgggcgacgc gatccagtat ctcgaggacg gcatgccggt cgaggtcgtg ttctacgacg
      121 gcaaggcgat ctcggtcgag ctgcccacca gcctggtgcg cgagatcacc gacaccgagc
      181 ccgccgtcaa gggcgacacc tcgggcaagg tgctgaagag cgcgaagatc gccaccggct
      241 tcgagatctc ggtgccgctg ttcgtgcaga ccggcgacaa ggtcgagatc gacacccgca
      301 cgcacgagta ccgcaagcgc gtttgaagac gagcgcggca ggcgatgaag ggtccgcgct
      361 gcgggccctt ctttcttgtg cgcgatgatc cgcccatgtc gttcgacttc gccgccgtcc
      421 gcgcgccgtt ccgcatgcag cccggcctgc gccggcttgc cgcgggcagc gcgccgctga
      481 cgccgaacct gcccggcgcg ccggcactgg tcgagaagac catcgtgctg cgagaccgcg
      541 ccggcgaggc gctggtcacc gcgcccgggt tcgacgccgg tccggccctg cagacgctga
      601 tgcagcaggc ggtacttgat cagccccatt gcttcgactg ggacggcgcg cacggcttcg
      661 aggcgcgact gctcggctgg tcggtgcgcg gcgccgaggt cgcgggcgac gggccacgcg
      721 aactcggcga gctgctgcgc gcgctgcccg ccgcgtggcg cctgcccggc ctgctgagcc
      781 tggcgctggc cgaagacttt gcggtgatcg atggcgcgag cgcgcgcatc ccgtggctcg
      841 ccgtgtgcct gccgtcgcgc tgggcgcccg ccgacaaggt cggccgccac ttcgtcgagg
      901 tgcacgcacc ggtggccgac aacgcgctgc tgctcggcgc ggccgatcac ctcgcgcagc
      961 tcgtgaccgg caccgagcgt tgggagcgct tcgtctggag catcagcagc gacgcccggc
     1021 tcgcgcagca cccgaagcat ggagcgccgc cgggctggcc ggccgcgctc gatgccgaag
     1081 cgctcgccgg cgccgcgcac ttccgcagcg aacaccagac cttcatcccg atcgcgtcgg
     1141 tgcgccaggc ggtgttcacg atcctggtcg agagccggcc gctgaccgaa gtcatcgact
     1201 cgcccgagcg cgcgcgccag cttcgcgacg cgctcgcgag catgagcgat gcggtgctgg
     1261 cctaccgcgg gctggtcgac gcgcgcgagc gcctgctggc ctggctcgcc gcgcgcagcg
     1321 catgaacagc gcgccggtcg tgccgggcac gctggcgttc gacgaccacg gcgtgccgcg
     1381 ctcgccgctg tacggcgata tctaccaccc gcgcagcggt gcaccggcgc aggcgcgcca
     1441 cgtctttctc ggcggcaacg aattgccgcg gcgctggcag ggccgcgacc gcttcgtcgt
     1501 gctcgagacc ggcttcgggc tgggccacaa tttcctcgcc acctggcagg cctggcgcga
     1561 cgacccgcag cgctgcgagc gcctggtctt catcgcgatc gagcagcatc cgctgacgct
     1621 ggacgatctg cgttcggtgc accgccacag cgccttgccc gaacttgcgg acgcgctgca
     1681 gcgcgcctgg ccgccgctga cccacaacct gcaccggctc gggttcgacg gcgacgcggt
     1741 cgaactgctg ctggccttcg gcgacgtgca ggtctggctg cccgaactcg tagccgaggt
     1801 cgatgcgttc tacctggacg gcttcgcgcc cgcgctgaac ccgcgcatgt gggacgaacg
     1861 ggtctgcaag gccctgggcc ggctggccac gccgggtgcg acgctggcga cctggagcgc
     1921 ggcgcacgca ctgcgccggc atctgacgag cgccggcttc gaggtccggc tcggtgcggg
     1981 tcagggcggc aagcgcgaca tcacgctggc ccgctacgcg ccgggcttca caccgcggcg
     2041 cacgccggcg cgccagggtc gcggtgccga cgccgacgac cggcacgcgt tgatcgtcgg
     2101 tgccggtctc gccggctgcg ccgccgcctg cgcgctcgca cggcaaggct ggcgcagcac
     2161 cgtgctcgag cgccgccgcg cgccggcgtc ggaagcctcc ggcaaccccg ccggcctgtt
     2221 ccacggcatc gtcaacgcgc aggacggcgt ccatgcgcgc ttcaaccggg ccgcggcgct
     2281 cgcggcgcac gccgctgtga gcgcggcgct cgcgcacggt gtgagtggag gcacgacggg
     2341 tctgctgcga ctggagacca cgctcgacgc ggtcgggatg cacgcgctgc tgcagcgcct
     2401 cgggctgccg cccgactacg tgcaggcgct cgatgcgagt gcggccagcg cgcgcgccgg
     2461 cctcgcgctc gcgcacccgg cctggttcta tccggccggc ggctgggtcg acccggcggg
     2521 tctggcgcgc tggttcctcg cacgcgcggc gaacagggtc gagcttcgcg ccgatgtcca
     2581 tgtgcattcg ctgcggcgca tcgacagcgg ctgggcgctg ctcgacgcgg ccgggcggac
     2641 gatcgcacag gcctgcaccg tcgtgctcgc gaacgccgcc gacgcgctgc gtttgctcgg
     2701 cgcacccgac tggccgatcg agagcgtgcg cggccagatc agcatcgcca gcgcgacgcg
     2761 gctcgccacg ccgcgcctgc cggtggcggg cgccggctac ctgctgccgg cgagcgcggg
     2821 ccgggcgatg ttcggtgcca ccgcacaacg cggcgatgcc gacgacagcg tgcgtgccgc
     2881 cgaccacgtc ttcaacctcg cgcaactcgc gcgcctgacc ggccggccga ccgggctgcg
     2941 cgtggacgaa ctcaccggcc gcaccgcgtg gcgctgcagc gccaccgatc gcctgccgct
     3001 gatcggcgcg gtgcccgacg acaacgctgc ggcatccgcg cgcctcgacc agccgcgctt
     3061 cgtgccgcgc ctgccgggcc tgttcatgtt cagcgcgctc ggttcgcgcg gcatcacctg
     3121 gagcgcgctc ggcgcgcagc tgctggcctc tgtggtgacc ggcgcgccgg cgccggtcga
     3181 ggcgagcctg ctcgacgccg tcgacccggc gcgcttcgtc agccgtcggg cgcgcctagc
     3241 cagcgccgga tgacggcgta cacgcgcggg tggttcagca ggctcatgtg accgagcccg
     3301 gcgagttcga cgcgctgaac gtcgccgctc gccgcgtcgt cggctgcgct gccggcgtgc
     3361 accaggccgt cgccgagcag gctgccgacg agcggcccga ggccggtgcc ggcttcgtcg
     3421 ccgagcgtgc cgaacaccag ccgcagcgcc ggcccgtcgg gcgcgcgcgg cgcggaccga
     3481 agcccgcgcc gcaagtcctt gatgccgcgg ctgcgcgcat cgacgatgcg cccgagcggc
     3541 ggggtcacct ccgacacgtt cagcgccgcc gcggcgaagt gcccggcctg cgccagcgat
     3601 gcgccgagat gcggtgaccc gaggcagacg atcatcggta ctcggcgcgc ccagcccagg
     3661 ccctgtgcgg tggccagcgc gtgtgcgcgt cgtgcgacca gcccgcccat gctgtgaccg
     3721 atcagcagcc actcgtgcac ctgcggggtg ccctgcgcca cctgcaccat caggtcggcg
     3781 agctgctgcg cattgccctc gatcgccagg ccgctgttgt agcgcagcca gacggcgctg
     3841 acaccgagtt cgtgctcgag caaggcgccg tagtcgatcg gctcgctggc atgcaaggtc
     3901 gacgcccacg gcgacgcggc ccacgcgtcg gtgaccaggc gccagctctg ctcgtcgcag
     3961 gccaggccgt gcaggaacac gcagacccgc ggtcgcagac ttgcgagcgc ctcgggcgtg
     4021 agcggctcgc ggcgaccgtg caagcccatc tgtaccgcga gcgcgctgcc ggaggccgcg
     4081 agcgtgtcgc cgaacgcgcc gctcagcgcg ccgcgcaacg cctgttcctt gtggccgagc
     4141 gcgcgggtcg ggtcgtcggc atggcgttcg acccctgcgg ccagcgccag cgtggccgcg
     4201 ccaccgtggc gcaccgccgc gtagacgcct tgcgtgatcg catcgtgcac accccgcacg
     4261 atacgcgtcg gcatcgccag cccgggcagc tgcagcaggc cgtcgaaggt cttgccggca
     4321 atcgcctggt gcatctcctg cacccgcgcg gtcgtggcct ggaagccggc gttcagggcc
     4381 ggtacataac cgggggcgcg cgccagaggg gcggcgggcg gaggtgctgg cagcgccgct
     4441 gcgcgcgaag gcgggcggcg cttgcccatg tcaggggcct gggaccggcg cgtcggccgg
     4501 cggggcgccg ctcaccgtgt cgtcatccgc gggggcatcg tcgccgcccg gttcgccgac
     4561 cttgcgaccg gccttctcct tcagcttctc ttccttcttc ttcttcttcg ccaactcgcg
     4621 ctggcgcttt tcgaacgaat aatttggttt ggccatgggc cgtccttcct gtctgctgag
     4681 ggttggcacc gctgatgcgc cagggccgga ggttacgcgc ttttcgcgcg gccccaaccg
     4741 gcgcggcgcc gaacgcgctc agaaagtcag ggtgcgcacg ccttgcgcgg tgccgagcag
     4801 gcacacgtcg gccttgtggc gcgcgaagat cccgaccccg accacgcccg gccattgatt
     4861 gatctcggac tcgagcgcgg ccgggtcggc gatcgacagc ccgcgcacgt cgatgatctg
     4921 gccgccgttg tcggtgacga cc
//
LOCUS       DATMJB010000673         2615 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_419704, whole genome shotgun sequence.
ACCESSION   DATMJB010000673 DATMJB010000000
VERSION     DATMJB010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419704"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VNU48_14285"
     CDS             <1..826
                     /locus_tag="VNU48_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWI12498.1"
                     /translation="ARDDVPLVPMICQLLSNERDPLKGRQLPVMYSMKRAGFFSISGN
                     LATQFIQAVGWAMASAIKGDTRIASAWIGDGATAEADFHTALTFAHVYRAPVILNVVN
                     NQWAISTFQAIAGGEGTTFAGRGVGCGIASLRVDGNDFLAVHAASTWAAERARSNLGP
                     TLIEWLTYRAGPHSTSDDPSKYRPADDAARFPLGDPIARLKQHLIALGAWSDAQHTAL
                     HSELTAEIGAAQKEAEAFGTLADGHVPPLASMFEDVYREMPAHLQAQLRQLQAAKG"
     gene            838..1842
                     /locus_tag="VNU48_14290"
     CDS             838..1842
                     /locus_tag="VNU48_14290"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017423187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWI12499.1"
                     /translation="MTMIQALRSAMDVMLERDPDVVVFGQDVGYFGGVFRCTEGLQAK
                     HGSARVFDTPISEGGIVGAAVGMGAYGLRPVAEIQFADYFYPASDQIVSEAARLRYRS
                     VGDFTAPITIRMPCGGGIYGGQTHSQSPEALFTHVCGLRTLMPSNPYDAKGLLISAIE
                     CDDPVIFLEPKRLYNGPFDGHHDRPVVPWTQHALAEVPEGHYRVPLETATVFRAGRAL
                     TVLTYGTMVFVSEAAANETGIDAEIIDLRSLWPLDLPTIVNSVKKTGRCVIVHEATRS
                     CGLGAELLSLVQEHCFYHLEAPIERVTGWDTPYPHALEWDYFPGPARVAAALQRAMEA
                     "
     gene            1842..>2615
                     /locus_tag="VNU48_14295"
     CDS             1842..>2615
                     /locus_tag="VNU48_14295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWI12500.1"
                     /translation="MGVHVIKMPDIGEGIAEVELVAWRVQPGDTVAEDQILADVMTDK
                     ATVEIPSPVVGTVLALGGEVGQVLAVGAELIRLEVADADGAVAKKAPAKAAAPAAPPA
                     RAATPRAVMPAPVAVSTAPAGERPIASPALRRRAWELGVDLNAVAGSGPGGRIMQADL
                     DAHAARAAPAHAPAAPARADEQLRITGLRRRIAQKMQESKRRIPHYTYVEEVDVTELE
                     ALRARLNDQHGAARGRLTLLPLLARAIVLGVRDFPQINAR"
BASE COUNT          380 a          925 c          958 g          352 t
ORIGIN      
        1 ggcgcgcgac gacgtgccgc tggtgccgat gatctgccag ctgctgtcga acgaacgcga
       61 cccgctgaag ggccgccagc tgccggtgat gtattcgatg aagcgcgccg gcttcttctc
      121 gatcagcggc aacctcgcga cgcagttcat ccaggcggtg ggctgggcga tggcgtcggc
      181 gatcaagggc gacaccagaa tcgcgtcggc ctggatcggc gacggcgcca ccgccgaggc
      241 cgacttccac accgcgctga ccttcgccca tgtgtaccgc gcgccggtga tcctgaacgt
      301 ggtcaacaac cagtgggcga tctcgacctt ccaggcgatc gccggcggcg aaggcacgac
      361 cttcgcgggc cgcggcgtgg gctgcggcat cgcctcgctg cgcgtggacg gcaacgactt
      421 cctcgcggtc catgcggcgt ccacctgggc cgccgagcgc gcgcgcagca acctcgggcc
      481 gaccctgatc gagtggctca cctaccgcgc cggcccgcac tcgacctcgg acgacccgtc
      541 caagtaccga cccgccgacg acgcggcgcg cttcccgctc ggcgacccga tcgcgcgcct
      601 gaagcagcac ctgatcgcgc tgggcgcgtg gtccgatgcg cagcacacgg cgctgcacag
      661 cgagctgacg gccgagatcg gcgcggcgca gaaagaggcg gaagccttcg gcacgctcgc
      721 cgacggccac gtgccgccgc tggcatcgat gttcgaggat gtctaccgcg agatgccggc
      781 gcacctgcag gcgcagctgc ggcagttgca ggcggcgaag ggctgagcat ggccttgatg
      841 acgatgatcc aggcgctgcg ctcggcgatg gacgtgatgc tggagcgcga cccggacgtg
      901 gtcgtgttcg gccaggacgt gggctacttc ggcggcgtgt tccgctgcac cgagggcctg
      961 caggccaagc acggcagcgc gcgcgtgttc gacacgccga tctccgaggg cggcatcgtc
     1021 ggcgcggcgg tcggcatggg cgcgtacggg ctgcggccgg tggccgagat ccagttcgcc
     1081 gactacttct acccggcctc ggaccagatc gtctccgagg cggcgcggct gcgctatcgc
     1141 tcggtgggcg acttcaccgc gccgatcacg atccgcatgc cctgcggcgg cggcatctac
     1201 ggcggccaga cccacagcca gagcccggag gcgctgttca cgcacgtctg cgggttgcgc
     1261 accttgatgc cgtcgaaccc gtacgacgcg aaggggctgc tgatctcggc gatcgagtgc
     1321 gacgacccgg tgatctttct cgagcccaag cggctgtaca acgggccctt cgacggccac
     1381 cacgaccgcc ccgtcgtgcc ctggacgcag cacgcgctgg ccgaggtgcc cgagggccac
     1441 taccgcgtgc cgctcgaaac ggccacggtg ttccgcgccg ggcgggcgct gacggtgctg
     1501 acctacggca cgatggtgtt cgtcagcgag gccgccgcga acgagaccgg catcgacgcc
     1561 gagatcatcg acctgcgcag cctgtggccg ctggacctgc cgacgatcgt gaactcggtg
     1621 aagaagaccg gccgctgcgt gatcgtgcac gaggccacgc gcagctgcgg gctgggggcc
     1681 gagctgctgt cgctggtgca ggagcattgc ttctaccacc tcgaggcgcc gatcgagcgc
     1741 gtgaccggct gggacacgcc ctacccgcac gcgctcgaat gggactactt ccccggcccg
     1801 gcgcgcgtgg ccgccgcgct gcaacgcgcg atggaggcct gatgggcgtg catgtgatca
     1861 agatgcccga catcggcgaa ggcatcgccg aggtcgaact cgtcgcctgg cgcgtgcagc
     1921 cgggcgacac ggtcgccgag gaccagatcc tggccgacgt gatgaccgac aaggcgaccg
     1981 tcgagatccc gtcgccggtg gtcggcacgg tgctggcgct gggcggcgag gtcgggcagg
     2041 tgctggcggt gggtgcggag ctgatccgcc tggaggtcgc cgacgcggac ggcgcggtgg
     2101 cgaagaaggc gcccgcgaag gcggctgcgc ctgcggcgcc acccgcgcgt gcggcaacgc
     2161 cacgcgccgt catgcccgcg ccggtggcgg tctcgaccgc gccggccggg gagcggccga
     2221 tcgcgtcgcc ggcgctgcgg cggcgcgcct gggagctcgg cgtcgatctg aacgcggtcg
     2281 cgggcagcgg cccgggcggc cgcatcatgc aggccgatct ggacgcgcac gcggcacgcg
     2341 ccgcaccggc gcacgcgcct gccgcgccgg cgcgtgccga cgagcagctg cgcatcaccg
     2401 gcctgcggcg gcgcatcgcg cagaagatgc aggagtccaa gcgccgcatc ccgcactaca
     2461 cctatgtcga ggaggtcgac gtcaccgagc tcgaagcgct gcgcgcgcgg ctgaacgacc
     2521 agcacggcgc cgcgcgcggg cgcctgaccc tgctgccgct gctggcgcgc gcgatcgtgc
     2581 tgggcgtgcg cgacttcccg cagatcaatg cgcgc
//
LOCUS       DATMJB010000674         2336 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_269027, whole genome shotgun sequence.
ACCESSION   DATMJB010000674 DATMJB010000000
VERSION     DATMJB010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269027"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VNU48_14300"
     CDS             complement(<1..312)
                     /locus_tag="VNU48_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mpo1-like protein"
                     /protein_id="HWI12501.1"
                     /translation="MRAATDLLSGYAQYHRDQRNIASHFVGVPMIVFGIGVLLARPAF
                     ALGGLALSPAWIAFALAAAWYLSRGNLVLGVAVSAGVGALLLAAHQVAYGSLGAWLAW
                     GV"
     gene            459..1028
                     /gene="dcd"
                     /locus_tag="VNU48_14305"
     CDS             459..1028
                     /gene="dcd"
                     /locus_tag="VNU48_14305"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HWI12502.1"
                     /translation="MSIKSDKWIRRMAETTGMIEPFEPGQIRADAQGHKIVSYGTSSY
                     GYDIRCAPEFKVFTNIYSTVVDPKNFDEKSFVDIHADVCVIPPNSFALARTLEYFRIP
                     RNVLTICLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGCAQVLF
                     FESDEVCETSYKDRGGKYQGQRGVTLPKT"
     gene            1196..2077
                     /locus_tag="VNU48_14310"
     CDS             1196..2077
                     /locus_tag="VNU48_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HWI12503.1"
                     /translation="MRMDGQEESSNVEDARGSGGGGGGGFRLGGGGIGLGSVAIALVA
                     GWIFGINPLTILGLLSGGGGAPMQQTAPGPAPAPPAGDAQARFVSQVLRSTEVVWTDV
                     FRQAGKTYQAPKLRLFSGSEPTACGRGDAAMGPFYCPGDRKVYLDMSFFNVMSQRLGA
                     PGQFAQAYVVAHEVGHHVQNLLGITDKVDAARERGSEAQANAMSVRVELQADCFAGVW
                     AKRSQAEQGWRLEQGDIETALNAASQIGDDTLQRKSRGTVVPESFTHGTSAQRVGWFK
                     RGFDSGSTDNCNTFEAR"
BASE COUNT          392 a          821 c          807 g          316 t
ORIGIN      
        1 cacgccccag gccagccagg cgccgaggct gccataggcg acctgatgcg ccgccagcag
       61 cagcgcgccg acgccggcgc tgaccgcgac gccgagcacg aggttgccgc ggctcaggta
      121 ccaggccgcg gccagcgcga acgcgatcca cgccgggctc aacgccaggc cgccgagcgc
      181 aaacgccggc cgcgcgagca gcacgccgat gccgaacacg atcatcggca cgccgacgaa
      241 atggctggcg atgttgcgtt ggtcgcggtg gtattgggca tacccgctga gcaggtcggt
      301 ggcagcgcgc atcacgggtc tccgttcggg gcgaggcatt tgacgcgcca atggtaggcc
      361 aatcgccata cccgtgccac ctgcagcccc ggggggccgc gcgcgatacc gaacaaggcc
      421 ttccctacaa tcgcccatct tccgcaaggg ccccgcgcat gagcatcaag agcgacaaat
      481 ggatccggcg catggccgag accaccggca tgatcgaacc cttcgagccg ggccagattc
      541 gcgccgacgc gcagggccac aagatcgtca gctacggcac cagcagctac ggttacgaca
      601 tccgctgcgc gcccgagttc aaggtcttca cgaacatcta cagcaccgtg gtcgacccga
      661 agaacttcga cgagaagagc ttcgtcgaca ttcacgccga cgtctgcgtg atcccgccga
      721 acagcttcgc gctggcgcgc acgctcgagt acttccgcat cccgcgcaac gtgctgacga
      781 tctgcctggg caagagcacc tacgcgcgct gcgggatcat cgtcaacgtc acgccgttcg
      841 agcccgagtg ggagggctac gtgacgctcg agttcagcaa caccacgccg ctgccggcca
      901 agatctacgc cggcgagggc tgcgcgcagg tgctgttctt cgagagcgac gaggtctgcg
      961 agaccagcta caaggaccgc ggcggcaagt accagggcca gcgcggcgtg acgctgccga
     1021 agacctgagg caccggcgcg cacgagggcg atgtcgctgc agccgcgggc gcggcggctg
     1081 gatgtgctgg ccgacccgcc ggccgtcgcc tgacgcagcg tgccaggcgc taccatcctc
     1141 gcacgccgcc ccggggcgcg tcgaccccgg cacgcgaacc gcgaaggagc aggcaatgag
     1201 gatggacggc caggaagaga gctcgaacgt cgaggacgcg cgcggatcgg gcggcggggg
     1261 cggcggcggg ttccggctcg gcggcggcgg gatcggcctg ggcagcgtcg cgatcgcgct
     1321 cgtcgccggc tggatcttcg gcatcaaccc gctcaccata ctgggtctgc tcagcggtgg
     1381 cggcggcgcg ccgatgcagc agaccgcgcc ggggccggca ccggcgccgc cggccggcga
     1441 tgcgcaggcc aggttcgtgt cgcaggtgct gcgcagcacc gaggtcgtct ggaccgatgt
     1501 gttccgccag gccggcaaga cctaccaggc gccgaagctg cgcctgttca gcggcagcga
     1561 gccgaccgcc tgcggccgtg gcgacgccgc gatgggcccg ttctactgcc ccggcgaccg
     1621 caaggtctac ctcgacatga gcttcttcaa cgtgatgagc cagcgcctgg gcgcgccggg
     1681 ccagttcgcg caggcctatg tggtcgcgca cgaggtcggc caccacgtgc agaacctgct
     1741 gggcatcacc gacaaggtcg acgcggcgcg cgagcgcggc agcgaggccc aggccaacgc
     1801 gatgagcgtg cgcgtcgagt tgcaggccga ctgcttcgcc ggcgtctggg ccaagcgctc
     1861 gcaggccgag cagggctggc ggctggagca gggcgacatc gagaccgcgc tgaacgccgc
     1921 ctcgcagatc ggcgacgaca cgttgcagcg caagtcgcgc ggcacggtcg tgcccgagag
     1981 cttcacgcat ggcaccagtg cgcagcgggt cggctggttc aagcgcggct tcgattccgg
     2041 cagcaccgac aactgcaaca cgttcgaagc gcgttaacgc tgattggggc cgtcatctca
     2101 gttgcttcac cgcgctgcgg gcgacccacg acgcacgaaa cgctgctgcg gctttggatc
     2161 tctgcttgcg gcccaatgct gtcggtgaat gtccaccgtg cacgtattgg cgcgggatcg
     2221 gaccggcggg gcatgctgct gcgctcatgt cccccggggt cggcctgcgg ccaacccctc
     2281 ctcctttact tcgctccgca gcacgcccca ccggcccgat ctgctccacc gtgcag
//
LOCUS       DATMJB010000675         2668 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_396595, whole genome shotgun sequence.
ACCESSION   DATMJB010000675 DATMJB010000000
VERSION     DATMJB010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396595"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..216)
                     /locus_tag="VNU48_14315"
     CDS             complement(25..216)
                     /locus_tag="VNU48_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12504.1"
                     /translation="MTRPLHLQHDRSASRGAACVGAAWIDSGIAPGLVVVDSSRPSPV
                     AGLVAGHARGLRKQFGAAH"
     gene            481..1260
                     /locus_tag="VNU48_14320"
     CDS             481..1260
                     /locus_tag="VNU48_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12505.1"
                     /translation="MVATALAVVLGLHGPIAQWAGYHAFADGRAWFGVPNAENVLSNL
                     PFALVGGWGLWAVAHLPQARGGRSAWACFGAAVLCTAFGSALYHWAPSNGALVFDRLP
                     IAWACAALTCALLAERVDARWASPAALTLALLVATASVAWWWLGEHRGVGDLRAYLFV
                     QFLPMALVPAAIGLKLPRGAAGGGLRDRDWWIVLGLYALAKLTELADRPVLEALGLVS
                     GHTLKHLFAAAAALWLLRAATRPLRRPRALATAPIGRTQLR"
     gene            complement(1248..2477)
                     /gene="argJ"
                     /locus_tag="VNU48_14325"
     CDS             complement(1248..2477)
                     /gene="argJ"
                     /locus_tag="VNU48_14325"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWI12506.1"
                     /translation="MPVNLQAPDPRSLHPVAGIELGIAMAGVRKANRRDLTVVTIAAG
                     STVAGVFTRNRFCAAPVQLCRTHLAAGAGARAIVVNTGNANAGTGADGLARARATCTA
                     LAGLLKVAPEQVLPFSTGVIMESLPVERVIAGLPAAIADLRADHWGAAAEAIMTTDTL
                     PKAASAQLQIGGRTVTITGISKGAGMIRPNMATMLGFVATDAAIAPALVQALVSDAAD
                     ESFNRITIDGDTSTNDSFLLIATAQAGNPEITQFASGDGKRLRDALVDVARRLAQAIV
                     RDGEGATKFITVQVDGGASEAECRQVAYAIAHSPLVKTAFFASDPNLGRILAAVGYAG
                     IADLDQTTIDLFLDEVHVAQGGARLPTYREEDGARVMQQSEITVRVDLHRGAASTVVW
                     TCDLSHDYVTINADYRS"
BASE COUNT          346 a          919 c          974 g          429 t
ORIGIN      
        1 gttcagaagc ggatgccgag aggactaatg cgcagcgccg aactgcttgc gcagcccgcg
       61 tgcgtggccg gcgacgaggc cggcgaccgg ggacggtcgg ctcgagtcga cgaccacgag
      121 cccgggcgcg atgcccgagt cgatccaggc cgcacccacg cacgcggcgc cgcgcgaggc
      181 gctgcgatcg tgctgaaggt ggaggggccg ggtcatgtcg tggtctcgat caatggggag
      241 cttgcatcgt agcagcggcg ccggggtgcg ggcaagaccg tgccgcggcg cagcgttgca
      301 ttccatccac gcgcccacgg tgtcggtcgc tgataccgca gggagcgaca gacgctgccg
      361 cgcatcgcgc cgcttgcggg tctcgcacca ccggcggcac gatgtcgtca cctctttcgt
      421 ccaccaccat ctgccatgag tacacgcctc cgattccccc gatctgtcgc ctggttgccg
      481 gtggtcgcga cggccctcgc ggtcgtgctc ggcctgcacg ggccgatcgc gcaatgggcc
      541 ggttaccacg cgttcgccga tggccgcgcc tggttcggcg tgcccaacgc cgagaacgtg
      601 ctgagcaacc tgcccttcgc gcttgtcggt ggctggggcc tgtgggccgt ggcgcatctg
      661 ccgcaggccc gcggcgggcg ctcggcctgg gcctgcttcg gcgcggccgt gctgtgcacc
      721 gcgttcggct cggcgctgta ccactgggcg cccagcaatg gcgcgctggt gttcgaccgg
      781 ctgccgatcg cctgggcctg cgcggcactg acctgcgcat tgcttgccga gcgtgtcgac
      841 gcgcgctggg cgtccccggc cgcgctgacc ttggcgttgc tcgtcgccac agcgtcggtg
      901 gcgtggtggt ggctgggcga acaccgcggt gtcggcgacc tgcgcgccta cctgtttgtg
      961 cagttcctgc cgatggcgct ggtaccggcc gccatcggcc tgaagttgcc acggggcgct
     1021 gcgggcggtg gcctgcgcga ccgcgactgg tggatcgtgc tcggcctgta cgcgctggcg
     1081 aagctgacgg aactcgccga ccgaccggtg ctggaggcgc tgggcctcgt cagcggacac
     1141 acgctcaagc acctgttcgc ggcagcagcc gcgctgtggc tgctgcgcgc ggcgacgcgg
     1201 ccgctgcgtc gcccacgtgc actcgcaacc gcgccaattg ggcggactca gctgcggtag
     1261 tcggcgttga tcgtgacgta gtcgtggctc aggtcgcagg tccagaccac ggtcgacgcc
     1321 gcgccgcggt gcaggtcgac gcgcaccgtg atctcgctct gctgcatcac ccgcgcgccg
     1381 tcctcctcgc ggtacgtggg caggcgagcg ccgccctgcg cgacatgcac ctcgtcgaga
     1441 aagaggtcga tcgtggtctg gtcgaggtcg gcgatgccgg catagccgac cgccgccaga
     1501 atccgcccga ggttcgggtc gctcgcgaag aacgcggtct tcaccagcgg cgagtgcgcg
     1561 atcgcatagg cgacctggcg gcattcggcc tcgctggcgc cgccatcgac ctgcaccgtg
     1621 atgaacttgg tcgcgccctc gccgtcgcgc acgatcgcct gcgccaggcg gcgcgcgacg
     1681 tcgacgagcg catcgcgcag ccgcttgccg tcgccggacg cgaactgcgt gatctcgggg
     1741 ttgccggcct gggcggtggc gatcagcagg aacgagtcgt tggtcgaggt gtcgccgtcg
     1801 atcgtgatcc ggttgaagga ctcgtcggcg gcgtcgctca cgagcgcctg caccagcgcc
     1861 ggcgcgatcg ccgcgtcggt cgcgacgaag ccgagcatcg tggccatgtt cgggcggatc
     1921 atcccggcgc ccttgctgat gccggtgatc gtcacggtcc ggccgccgat ctgcagttgc
     1981 gcagaagcgg ccttcggcag cgtgtcggtg gtcatgatcg cctcggcggc cgcgccccag
     2041 tggtcggcac gcaggtcggc aatcgcggcg ggcagcccgg cgatgacgcg ttcgaccggc
     2101 aggctttcca tgatcacgcc ggtcgagaac ggcagcacct gctccggcgc gaccttcagc
     2161 aggccggcca gcgcggtgca ggtggcgcgc gcgcgggcca ggccgtcggc cccggtgccg
     2221 gcgttggcgt tgccggtgtt gacgacgatc gcgcgcgcgc cggcgccggc cgccaggtgg
     2281 gtgcggcaca gctgcaccgg cgccgcgcag aagcggttgc gggtgaacac gcccgcaacc
     2341 gtcgagccgg ccgcgatcgt gacgaccgtc aggtcgcgcc ggttggcctt gcgcacgccc
     2401 gccatcgcga tgccgagctc gatgccggca acggggtgga ggcttctcgg atcgggggct
     2461 tgcaggttga cgggcatggc atctcacgaa aagcggcggc aatggcgggc gattgtgcat
     2521 tcatcgaaat cgccacgccg gcgtcgattg gtgccgcacc tgtaagccct gcctatttcc
     2581 agccttcgcg acctgcatta gagtcggccc gaaacatgca tcgggtcgcc gaccggaatg
     2641 catctcaacg ttgtcgggac ccctgaat
//
LOCUS       DATMJB010000676         3645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_328415, whole genome shotgun sequence.
ACCESSION   DATMJB010000676 DATMJB010000000
VERSION     DATMJB010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328415"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VNU48_14330"
     CDS             complement(<1..695)
                     /locus_tag="VNU48_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWI12507.1"
                     /translation="MPTAPLVSGILFALAAGLMWGLVFVGPLLLPEYPATLQSFGRYL
                     AFGLIALPLAWFDRDRLKQLARADWIEALKLALVGNIVYYLFLASSIQRAGGPLPTMI
                     IGTLPVVIAITSKLRRAAPGARVEAPLAWRRLLPSLALIALGIAIVNHVELERLRADP
                     HADLARYALGGLLAGGAVACWTWYPIRNAEWLRAHPDRSPRGWATAQGIATLPLALAG
                     YALTWAWFAASGSA"
     gene            complement(712..2124)
                     /locus_tag="VNU48_14335"
     CDS             complement(712..2124)
                     /locus_tag="VNU48_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWI12508.1"
                     /translation="MNHKLFRTRWALAPLFAALVLAGCATTPAFEPAANPAAPAAFRE
                     LDGRWAAVPPAEAQPRGQWWKAFSDPLLDDLIARAERNNTSIQLAAARLAQARALVRS
                     TDADRMPQIGLGAGANRQTASPAFGTTAPGTVLNAGVGASYEPDLFGRLAKASNAAAL
                     DAQSREALVQSTRLLVQSDVAQAYFGLRALDAERALVRQTVVAYRDTLHLTERRYQAG
                     DVAELDLARVRTEVAATESQALALDQRRAQLEHALAVLLGELPSGLTVAETEWNGTPP
                     LVPPGVPSTVLARRPDVAAAQRAMQAAQARVGIAQTAWFPDVALTASGGFASTDLGDL
                     FKWSARSWGIGALLSLPVFDGGRREAGLQNANAQWDAAAAGYREQVLVAFREVEDQLS
                     ALRLLADQAGVQAQAVDSATRATVLSTSRYRNGYVSQLELLDARRSELANRREALQVR
                     AAQYQATVGLIRALGGGWGA"
     gene            complement(2154..>3645)
                     /locus_tag="VNU48_14340"
     CDS             complement(2154..>3645)
                     /locus_tag="VNU48_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWI12509.1"
                     /translation="PDGATLDRTEDVVRRMNAIVNKQAGVEHAIAFPGLSINGFTNSS
                     NSGVVFVALKDFDQRKTRDLSAGAIAQSLNGQFGQIQDAFIAIFPPPPVQGLGTTGGF
                     KLQLEDRASLGYDALDAAQKAFMAKAYQTPELAGMFSSWQVNVPQLYADIDRTKARQL
                     GVAVTDVFDTMQIYLGSLYANDFNKFGRTYSVRVQADAAYRARAEDIGALKVRSTTGE
                     MVPLSALMKVTPTFGPERAMRYNGFLTADINGGPAPGFSSGEAQDAVRRIAAETLPAG
                     IAYEWTDLTYQEILAGNSAALVFGVAILLVFLVLAAQYESLTLPLAILLIVPMGLLAA
                     MTGVWLSDGDNNVFTQIGLMVLVGLSAKNAILIVEFARELEFAGRTPIQAAIEASRLR
                     LRPILMTSMAFVMGVLPLVLATGAGAEMRHAMGVAVFFGMIGVTAFGIFLTPVFYVLL
                     RRLTGNRPLKLHGGVPNVDPADAIGGAGGASHALSPQPAAALRFPE"
BASE COUNT          541 a         1291 c         1309 g          504 t
ORIGIN      
        1 gccgagccgc tcgcagcgaa ccaggcccag gtcagcgcgt agccggcgag tgcgagcggc
       61 aaggtcgcaa tgccctgcgc cgtggcccag ccgcgcgggc tgcggtcggg gtgcgcgcgc
      121 agccattcgg cgttgcggat cgggtaccag gtccagcagg cgaccgcgcc gcccgccagc
      181 agcccgccca gcgcgtagcg cgcgaggtcg gcgtgcgggt cggcccgcag tcgttcgagc
      241 tcgacgtgat tgacgatcgc gatcccgagc gcgatcagcg cgagcgacgg cagcagtcgg
      301 cgccacgcga gcggcgcctc gacgcgcgcg ccgggcgcgg cgcggcgcag tttcgaggtg
      361 atcgcgatga ccaccggcag cgtgccgatg atcatcgtcg gcagcggccc gccggcgcgc
      421 tggatgctgc tggccaggaa caggtagtag acgatgttgc cgaccagcgc gagcttcagc
      481 gcctcgatcc aatcggcgcg cgcgagctgc ttcagccggt cgcgatcgaa ccacgccagc
      541 ggcagcgcga tcagcccgaa cgcgaggtag cggccgaagg actgcaaggt cgccgggtac
      601 tcgggcagca acagcgggcc gacgaacacc aggccccaca tcagcccggc ggcgagggcg
      661 aacaggattc cggaaacgag cggggcggtc ggcatcgccc gcgagtgtcg ctcaggcgcc
      721 ccagccacct ccgagcgcgc ggatcagccc gaccgtcgcc tgatactgcg cggcgcgcac
      781 ctgcagcgcc tcgcgccggt tcgcgagctc gctgcggcgc gcgtccagca gttcgagctg
      841 gctcacatag ccgttgcgat agcgcgaggt ggacagcacg gtcgcgcgcg tcgccgaatc
      901 gaccgcctgc gcctgcacgc cggcctggtc ggcaagcagc cgcaacgccg agagctggtc
      961 ctcgacctcg cggaacgcca ccagcacctg ttcgcggtag cccgcggcgg cggcgtccca
     1021 ctgcgcgttc gcgttctgca ggccggcttc gcggcggccg ccgtcgaaca ccggcagcga
     1081 cagcagcgca ccgatgcccc aggaccgcgc cgaccacttg aacagatcgc cgaggtcggt
     1141 cgacgcgaag ccgccggatg cggtcagcgc gacgtcgggg aaccacgccg tctgcgcgat
     1201 gccgacgcgt gcctgcgcgg cctgcatcgc gcgctgcgcc gcggccacgt cgggccggcg
     1261 cgcgagcacc gtgctcggca cgccgggcgg taccagcggc ggggtgccgt tccactccgt
     1321 ctcggccacc gtcaggccac tcggcaactc gcccagcagc accgcgagcg cgtgttcgag
     1381 ctgggcgcgg cgctggtcca gcgcgagcgc ctgcgactcc gtggccgcga cctcggtgcg
     1441 cacgcgcgcg aggtcgagct cggcgacgtc gccggcctgg tagcgacgct cggtcaggtg
     1501 caaggtgtcg cggtaggcga cgacggtctg gcgcaccagc gcgcgctcgg catcgagcgc
     1561 acgcagcccg aagtaggcct gcgcgacgtc cgattgcacc agcagccgcg tgctctgcac
     1621 cagcgcctcg cgcgactgcg catcgagcgc ggcggcattg ctcgccttcg cgagccggcc
     1681 gaacaggtcg ggctcgtagg acgcgccgac gccggcattc aacaccgtgc cgggggcggt
     1741 cgtgccgaac gccgggctcg cggtctggcg attcgcgccc gcccccaggc cgatctgcgg
     1801 catgcggtcg gcatcggtcg atcgaaccag cgcacgcgcc tgcgcgagcc gcgccgcggc
     1861 gagttggatg ctggtgttgt tgcgctcggc gcgtgcgatc aggtcgtcga gcagcggatc
     1921 ggagaaggcc ttccaccact gcccgcgcgg ctgtgcctcg gcgggcggga ccgcggccca
     1981 gcggccgtcg agttccctga aggcggccgg cgccgcgggg ttggcggccg gctcgaacgc
     2041 cggcgttgtc gcgcagccgg cgagcacgag cgcggcgaag agcggcgcca gggcccagcg
     2101 ggttctgaag agcttgtggt tcatctcatg tccttcgaat ggtgtcgccg cgctcactcg
     2161 gggaagcgca gcgccgcggc cggctgcggg ctgagcgcgt ggctcgcgcc gccggcgccg
     2221 ccgatcgcgt cggccgggtc gacgttcggc acaccgccgt gcagcttcag cggccggttg
     2281 ccggtgagcc ggcgcagcag cacgtagaac accggcgtca ggaagatgcc gaacgcggtc
     2341 acgccgatca ttccgaagaa caccgcgaca cccatcgcat ggcgcatctc ggcgccggcc
     2401 cccgtggcga gcaccagcgg caagacaccc atcacgaacg ccatcgaggt catcaggatc
     2461 ggccgcagcc gcagccggct ggcctcgatc gcggcctgga tcggcgtgcg acccgcgaat
     2521 tccagctcac gtgcgaactc gacgatcagg atcgcgttct tcgcactcag tccgaccaac
     2581 accatcaacc cgatctgcgt gaacacgttg ttgtcgccgt ccgagagcca cacgccggtc
     2641 atcgcggcga gaaggcccat cggcacgatc aacaggatcg cgagcggcag cgtgaggctc
     2701 tcgtattgcg ccgcgagcac gaggaacacg agcaggattg ccaccccgaa gacgagcgcg
     2761 gccgagttgc cggcgaggat ctcctggtag gtcaggtcgg tccactcgta ggcgatgccg
     2821 gccggcagtg tctcggcggc gatgcgccga accgcgtctt gtgcctcgcc cgacgagaag
     2881 cccggcgccg ggccgccgtt gatgtcggcg gtcaggaagc cgttgtagcg catcgcgcgt
     2941 tccgggccga aggtcggcgt gaccttcatc agcgccgaca gcggcaccat ctcgccggtt
     3001 gtcgagcgta ccttcaacgc gccgatgtct tcggcgcgcg cgcggtaggc cgcatcggcc
     3061 tgcacccgca ccgagtaggt tcgaccgaac ttgttgaagt cgttcgcgta caggctgccg
     3121 aggtagatct gcatcgtgtc gaacacatcg gtcaccgcga cgccaagctg gcgcgccttg
     3181 gtgcggtcga tgtcggcgta gagctgtggc acgttgacct gccagctcga gaacatgccg
     3241 gccagctcgg gcgtctggta cgccttcgcc atgaaggcct tctgcgccgc gtcgagcgcg
     3301 tcgtagccga gcgaggcgcg gtcttcgagc tgcagcttga agccgccggt cgtgcccagc
     3361 ccctgcaccg gcggcggcgg gaagatcgcg atgaacgcgt cctggatctg gccgaactgg
     3421 ccgttcaggc tttgcgcgat cgcgccggcg ctcaggtccc tcgtcttgcg ctggtcgaag
     3481 tctttcagcg cgacgaacac gacgccggaa ttcgagctgt tcgtgaagcc gttgatcgac
     3541 agccccggaa acgcgatcgc gtgctcgacg ccggcctgct tgttgacgat cgcgttcatg
     3601 cggcgcacga cgtcttcggt gcggtcgagc gtcgcgccgt cgggc
//
LOCUS       DATMJB010000677         2363 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_624807, whole genome shotgun sequence.
ACCESSION   DATMJB010000677 DATMJB010000000
VERSION     DATMJB010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624807"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /gene="dacB"
                     /locus_tag="VNU48_14345"
     CDS             <1..1233
                     /gene="dacB"
                     /locus_tag="VNU48_14345"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HWI12510.1"
                     /translation="ASLMKLLTTYAALDLLGPAWTWSTPVWLQGRISDGVLHGDLVIK
                     GTGDPKLVLERMWLLLRRVQQLGVREIRGDIVLDRRAFVVPEQNPADFDGEALRPYNV
                     GADALLLNYRAVIYTIAPDAMRGVARIGAEPPLAGVRHDTSVPLTGGPCADWRGALRA
                     DFSDPRRVRFAGAYAAACGEQAWPVAAVEPARYDERALRGLWHEMGGRLRGTVRDGFA
                     PDTPPSFESRSPTLAELVRDINKYSNNVMAQQLFLTLGLTQRDAGTPEAARDVLRQWA
                     AQRFGSVAATLVLDNGSGLSRASRLSAQLLTRLLLAAWASPVMPELLGSLPVSGGYGT
                     LTGSQATLVRAHLKTGSLRDVAGIAGVVLADSGRRYVVVAIVNHPNASAARPALAALV
                     QWAADDATLPVTSAEPRH"
     gene            1250..1522
                     /locus_tag="VNU48_14350"
     CDS             1250..1522
                     /locus_tag="VNU48_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HWI12511.1"
                     /translation="MTLIDTLGYAAAALTTASFMPQAWLTFRTRDVSGISLAMYSVFT
                     FGIALWLAYGVLLRAWPIVIANGVTLALAAGILTMKLRLMDRPPRR"
     gene            complement(1534..>2363)
                     /locus_tag="VNU48_14355"
     CDS             complement(1534..>2363)
                     /locus_tag="VNU48_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWI12512.1"
                     /translation="GDEFSVINANGSKYTVNALLSGSSTAFDCAANPIWIQTLAATYG
                     LVFPQCAGTTPDPVSRIYAANGAMVGDISGQIDQQLNLGGGFAAGDLVTVFAGANDIV
                     AQFQQYPAVGEDQLTANLTVAAAALADQVNRLAGYGAKVLIVTVPDMGLTPFAGDRSV
                     GSTDGNPPLLTRLSTKFNDALLSSLLNDGHKIGLVQLDQYLKSVDTAAINGVSGAPFS
                     NTTRAACTVALPNCSTNTLVSDAVGATWLWADSRHLAAGGQASLGSLAQGRAHGNPF"
BASE COUNT          315 a          833 c          855 g          360 t
ORIGIN      
        1 gcctcgttga tgaagctgct gacgacctat gccgcgctgg acctgctcgg ccccgcgtgg
       61 acgtggagca caccggtgtg gctgcaaggg cgcatcagcg acggcgtgct gcacggcgac
      121 ctcgtgatca agggtaccgg cgacccgaaa ctcgtgctgg agcgcatgtg gttgctgctg
      181 cgccgcgtgc agcagctcgg cgtgcgcgag atccgcggcg acatcgtgct cgaccgccgc
      241 gccttcgtcg tgcccgaaca gaacccggcc gacttcgacg gcgaggcgct gcggccctac
      301 aacgtcggcg ccgatgcgct gctgctgaac taccgcgccg tgatctacac gatcgcgccc
      361 gatgcgatgc gcggcgtcgc gcggatcggg gccgaaccgc cgctggccgg tgtgcgccac
      421 gacacaagcg tgcccttgac cggggggccg tgcgcggact ggcgcggcgc gctgcgcgcc
      481 gacttcagcg acccgcggcg cgtgcgcttc gcgggcgcgt acgccgcggc ctgcggcgag
      541 caggcctggc cggtcgccgc tgtcgagccc gcgcgctacg acgagcgcgc gctgcgcggc
      601 ctgtggcacg agatgggcgg gcggctgcgc ggcacggtac gcgacggctt cgcgcccgac
      661 acgccgccga gcttcgaatc gcgctcgccg acgctggccg agctggtgcg cgacatcaac
      721 aagtacagca acaacgtgat ggcacagcag ctgttcctga cgctgggcct gacccagcgc
      781 gacgccggca cgcccgaggc ggcgcgcgac gtgctgcgcc agtgggcggc gcagcgcttc
      841 ggcagcgtcg cggcgacgct ggtgctcgac aacggctcgg gcctgtcgcg cgcatcgcgg
      901 ctcagcgcgc agttgctgac acggctgctg ctcgccgcct gggcgagccc ggtgatgccc
      961 gagctgctgg gctcgctgcc ggtcagcggt ggctacggca cgctcacggg ttcacaggcg
     1021 acgctggtcc gcgcgcacct gaagaccggg tcgttgcgcg atgtcgccgg catcgccggc
     1081 gtcgtgctcg cggattcggg gcggcgctat gtcgtcgtcg ccatcgtcaa ccacccgaac
     1141 gccagcgccg cgcgcccggc cctggccgcg ctggtgcagt gggccgcgga cgacgccacg
     1201 ctgcccgtca catcggccga accccgtcac tgagccccga gcgcccgaca tgaccctgat
     1261 cgacaccctc ggctacgccg ccgccgcgct gaccaccgcc tcgttcatgc cccaggcctg
     1321 gttgaccttc cgcacccgcg acgtgagcgg catctcgctc gcgatgtaca gcgtcttcac
     1381 gttcggcatc gcgttgtggc tggcctacgg cgtgctgctg cgggcctggc cgatcgtcat
     1441 cgccaacggc gtgacgctgg cgctggccgc cggcatcctg acgatgaagc tgcgtctgat
     1501 ggaccggccg ccgcgccgct gaaatcgcga tcctcagaac gggttgccgt gcgcgcggcc
     1561 ttgcgccagc gacccgaggc tggcctggcc gcccgccgcc agatggcggc tgtcggccca
     1621 cagccaggtc gcaccgacgg cgtccgacac cagcgtgttg gtgctgcagt tcggcagcgc
     1681 gacggtgcag gcggcccgcg tggtgttgct gaacggcgcc ccgctgacgc cgttgatcgc
     1741 cgcggtgtcg accgacttca ggtattggtc gagttgcacg aggccgatct tgtgcccgtc
     1801 gttgagcagg ctcgagagca gcgcgtcgtt gaacttcgtc gacaggcggg tcagcagcgg
     1861 cgggttgccg tcggtcgagc cgaccgagcg atcgccggcg aacggcgtca gccccatgtc
     1921 gggaacggtc acgatcagca ccttggcgcc gtagccggcg aggcggttga cctgatcggc
     1981 cagcgccgcc gcggcgacgg tcaggttcgc ggtcaactgg tcctcgccga ccgcggggta
     2041 ctgctggaac tgcgcgacga tgtcgttggc gccggcgaac accgtgacca ggtcgccggc
     2101 cgcaaagccg ccaccgaggt tgagctgctg gtcgatctgg cctgagatgt cgccgaccat
     2161 cgcgccgttg gcggcgtaga tgcggctgac cgggtcgggc gtcgttcccg cgcattgcgg
     2221 gaacaccagg ccgtaggtgg ccgcgagcgt ctggatccag atcgggttgg cggcgcaatc
     2281 gaacgccgtg gagctgcccg acagcagcgc gttgacggtg tacttgctgc cgttggcatt
     2341 gatgacgctg aattcgtccc cga
//
LOCUS       DATMJB010000678         4215 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_565866, whole genome shotgun sequence.
ACCESSION   DATMJB010000678 DATMJB010000000
VERSION     DATMJB010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565866"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="VNU48_14360"
     CDS             complement(<1..598)
                     /locus_tag="VNU48_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI12513.1"
                     /translation="MTSPDANLFSALRAGFPDDLDGVAITTVAATPLHYRWRDLERGT
                     AMLANLLDALELPAGSRVAVQTEKSVEALMLYLAVLRAGLVYLPLNTAYQREEIGYFI
                     VNAEPAVVVCAPKNFGWVSRIAFAAGTTHVFTLGDDRSGSLLQRAAVHSDRHTPAPRA
                     DDDLAAILYTSGTTGKAKGAMLTHGNLLSNARVLKDFWH"
     gene            complement(595..1383)
                     /locus_tag="VNU48_14365"
     CDS             complement(595..1383)
                     /locus_tag="VNU48_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWI12514.1"
                     /translation="MNGTILLQPDAGDGVARVVLSNPAKLNAIDIAMWRELRAVFDAL
                     QAEPPAHAPRAVLVSGAGGQFASGGDIQEFASFRFDEPRLHDFHEHIVAPALRAMLDC
                     DIPLFAQIDGACIGGGLEIAACCDIRICGAGSRFGAPIAKLGFPMAPGELELLARVAA
                     PPVLREMLLEARLFDAPRALQLGLVHEVVADAEVGARSLQRAQAAAALSPQAARLNKR
                     SLRQLAAGGPDARERRAHFDYAASAEHREGVMAFIEKRSPRFRP"
     gene            complement(1430..2728)
                     /locus_tag="VNU48_14370"
     CDS             complement(1430..2728)
                     /locus_tag="VNU48_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase"
                     /protein_id="HWI12515.1"
                     /translation="MLEELKNAARKVQGERELRGLMVDCRRLMSERGEANSVAIARQL
                     LARYGELPDEQRHAFFERLAHDFSPDPAAVLQSAQAYAKDPNAANLLRLTRLAEPPRQ
                     ELLRRINRSAGGTAGIVAMRRALLARLAKRPELQAVDADFRHLLSSWFNPGFLQLERV
                     DWNSPAQLLEQIIRHEAVHQIDGWDDLRRRLQPDRRCFAFTHPQLPNEPLIFVEVALL
                     PEMPGAIAPLIDKASEPLPPKQFKVAAFYSISNCQPGLRGVSMGNFLIKRVAEELRRE
                     LPQLRRFCTLSPIPGFAAWLRAGAPCPGLPKARAERLAQAQAALRAHGGADLSALANA
                     DALRRLDREAAEALLRLCAFYLALGSPTEHGDPVARFHLDNGARLERLNLLGDLSAKG
                     VKQSLGLMVNYLYDLDRVEAHHEKFVQGSVAQSRAVTTLL"
     gene            2802..>4215
                     /locus_tag="VNU48_14375"
     CDS             2802..>4215
                     /locus_tag="VNU48_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWI12516.1"
                     /translation="MSLHPATPPLPADADGVLKLAAQSMFETLASTAMGMLVVDREHR
                     IVWISEGYKQFLPGLEREESDFVGRRVEEVVPNTLMAQVIDSGQAILVDLLSNKAGTF
                     LVSRLPLRDANGVVIGAIGMVLLDHPETTMRPLTAKFAQLERDLTEARQELAAHLTQQ
                     RRPKHTIASFVGTSPAAVEVKRQARRVAQTDSTVLLLGETGTGKELLAHGIHAASRRA
                     AKPFIAVNIAAVPETLLEAEFFGVAPGAYTGAERKGRDGKFKLADGGTLLLDEIGDMP
                     LALQSKLLRVLQEQEVEPLGSNTVQRIDVRIIAATSRDLPAMIADGSFRADLYYRLNV
                     LPIRLPALRERIADLEALVESLAEDIARRSGLPHKSVTPDALDLLARHNWPGNIRELR
                     NALEQATLMTDDAVLDASHFAALVSGADGTHTATPTADAAAIAPRSAPVTPAAQAASN
                     DDRSAAITPLPQAIAELEART"
BASE COUNT          615 a         1480 c         1530 g          590 t
ORIGIN      
        1 agtgccagaa atccttcagc acgcgtgcgt tgctcagcag gttgccgtgg gtcagcatgg
       61 cgcccttggc tttgcccgtc gtgccgctgg tgtacaggat cgcggcgagg tcgtcgtcgg
      121 cgcgcggtgc cggcgtgtgg cggtcgctgt gcaccgcggc gcgctgcagc aggctgccgc
      181 tgcggtcgtc gccgagcgtg aacacgtgcg tcgtgccggc cgcgaatgcg atccggctga
      241 cccagccgaa attcttcggc gcgcagacca cgacggcggg ttccgcgttg actatgaagt
      301 agccgatctc ctcgcgctgg taggcggtgt tcagcggcag gtagacgagc ccggcgcgca
      361 gcaccgcgag gtagagcatc agcgcctcga ccgacttctc ggtctgtacc gcgacgcggc
      421 tgccggccgg cagctcgagc gcgtcgagca ggttcgcgag catcgcggtg ccgcgttcga
      481 ggtcgcgcca gcggtagtgc agcggcgtgg cagcgacggt cgtgatcgcc accccgtcga
      541 gatcgtcggg gaagccggcg cgcagcgcgc tgaacaaatt cgcgtctgga ctcgtcatgg
      601 tcggaatcgg ggggagcgtt tctcgatgaa ggccatcacg ccttcgcggt gctcggcgct
      661 ggcggcgtag tcgaagtggg cgcggcgctc gcgcgcgtcg gggccgcccg ccgcgagctg
      721 acgcaggctg cgcttgttca gccgcgcggc ctgcggcgac agcgccgccg cggcctgcgc
      781 gcgctgcagg ctgcgtgcac cgacctcggc atcggccacg acctcgtgta cgaggccgag
      841 ctgcagcgcc cgcggtgcgt cgaacaggcg cgcctcgagc agcatctcgc gcagcacggg
      901 cggcgcggcc acgcgcgcca gcagttcgag ctcgcccggc gccatcggga agccgagctt
      961 ggcgatcggc gcgccgaagc ggctgcccgc gccgcagatg cggatgtcgc agcaagccgc
     1021 gatctccagc ccgccgccga tgcaggcgcc gtcgatctgc gcgaacagcg ggatgtcgca
     1081 gtccagcatc gcacgcagcg ccggcgcgac gatgtgctcg tggaagtcgt gcagccgcgg
     1141 ctcgtcgaag cgaaagctcg cgaactcctg gatgtcgccg cccgaggcga actggccgcc
     1201 cgcgccgctg acgagcacgg cgcgcggcgc gtgcgccggc ggctcggcct gcagcgcgtc
     1261 gaacacggcg cgcagctcgc gccacatcgc gatgtcgatc gcattgagct tggccgggtt
     1321 ggagagcacg acgcgcgcca caccgtcgcc ggcgtcgggc tgcaggagga tggttccgtt
     1381 catgccgcca ttgtcgcgcc ggccgcgacg cgcgcgcggc gccgcacgct cacagcagcg
     1441 tcgtcacggc gcgcgattgc gcgacgctgc cctggacgaa tttctcgtgg tgggcttcga
     1501 cgcggtcgag gtcgtagagg tagttgacca tcaggccgag cgattgtttg acgcccttgg
     1561 cggacagatc gccgagcagg ttcaggcgct ccaggcgcgc gccgttgtcg aggtggaagc
     1621 gcgccaccgg gtcgccgtgt tcggtcggcg accccagcgc gaggtagaac gcgcacaggc
     1681 gcagcagcgc ctcggccgct tcgcgatcga gccggcgcag cgcgtcggcg ttggcgagtg
     1741 ccgacaggtc cgcgccgccg tgcgcgcgca gagctgcctg ggcctgcgcc agccgctcgg
     1801 cgcgcgcctt cggcaggccg gggcacggcg cgcccgcgcg cagccacgca gcgaacccgg
     1861 ggatcggcga cagcgtgcag aacctgcgca gctgcggcag ctcgcgccgc agttcctcgg
     1921 ccacccgctt gatcaggaag ttgcccatcg acacgccgcg cagccccggc tggcagttgc
     1981 tgatcgagta gaaggccgcg accttgaact gcttcggcgg aagcggttcg gacgccttgt
     2041 cgatcagcgg cgcgatcgcg cccggcattt cgggcagcag tgcgacctcg acgaagatca
     2101 gcggctcgtt cggcagctgc gggtgcgtga aggcgaagca gcggcggtcc ggctggagcc
     2161 gccggcgcag gtcgtcccag ccgtcgatct ggtgcacggc ttcgtggcgg atgatctgtt
     2221 ccagcagctg cgccggcgag ttccagtcga cccgctccag ctgcaggaag ccggggttga
     2281 accagctcga cagcaggtgc cggaagtcgg cgtccacggc ctgcagctcg ggccgtttcg
     2341 ccagacgtgc cagcagcgcg cggcgcatcg cgacgatgcc ggccgtgccg ccggcggagc
     2401 ggttgatgcg gcgcagcagc tcctggcgcg gcggttccgc cagccgggtc aggcgcagca
     2461 ggttggcggc gttcggatcc ttcgcgtagg cctgcgcgct ttgcagcacc gccgcggggt
     2521 cggggctgaa gtcatgcgcc aggcgctcga agaacgcgtg gcgctgctcg tccggcagct
     2581 cgccgtagcg ggccagcagc tggcgcgcga tggccacgct gttcgcttcg ccgcgctccg
     2641 acatcagccg ccggcagtcg accatcaggc cgcgcagctc gcgctcgccc tgcaccttgc
     2701 gggccgcgtt cttcaactct tcgagcatga actcctcgcg tcgggtggtg gcgcgtcgcc
     2761 gtctcagttt acggcgcgtg cgccgtaaac tgcggggccc gatgtcactg caccccgcca
     2821 cgccgccact gcccgccgat gccgacggcg tgctgaagct cgccgcgcag agcatgttcg
     2881 agacgctggc gagcactgcg atgggcatgc tggtggtcga ccgcgagcac cgcatcgtct
     2941 ggatcagcga gggctacaag cagttcctgc cggggctgga gcgcgaggag tcggacttcg
     3001 tcggccggcg cgtcgaagag gtcgtgccga acacgctgat ggcgcaggtg atcgacagcg
     3061 gccaggcgat cctggtcgac ctgctgagca acaaggcggg cacctttctg gtcagccgct
     3121 tgccgctgcg cgacgcgaac ggcgtcgtga tcggcgcgat cggcatggtg ctgctcgacc
     3181 acccggagac gacgatgcgc ccgctgacgg ccaagttcgc gcagctcgag cgcgacctca
     3241 ccgaggcacg ccaggaactc gccgcgcacc tgacgcagca gcgccgcccc aagcacacga
     3301 tcgcgagctt cgtcggcacg agcccggcgg cggtcgaggt caagcgccag gcgcgccgcg
     3361 tcgcgcagac cgacagcacg gtgctgctgc tcggcgagac cggcaccggc aaggaactgc
     3421 tcgcgcacgg catccacgcg gcgagccggc gtgccgcgaa gccgttcatc gcggtcaaca
     3481 tcgccgcggt gcccgagacg ctgctcgagg ccgagttctt cggcgtggcg ccgggcgcgt
     3541 acaccggcgc cgagcgcaag ggccgcgacg gcaagttcaa gctcgccgac ggcggcacgc
     3601 tgctactcga cgagatcggc gacatgccgc tggcgctgca gagcaagctg ctgcgggtgc
     3661 tgcaggagca ggaggtcgag ccgctcggca gcaacacggt gcagcgcatc gacgtgcgca
     3721 tcatcgcggc gaccagccgc gacctgccgg cgatgatcgc ggacggcagc tttcgcgccg
     3781 acctctacta ccgcctgaac gtgctgccga tccggctgcc ggccctgcgc gaacgcatcg
     3841 ccgatctgga agcgctggtc gaatcgctcg ccgaagacat cgcgcgacgc agcggcctgc
     3901 cgcacaagag cgtgacgccg gatgcgctgg acctgctcgc tcgccacaac tggccgggca
     3961 acatccgcga gctgcgcaac gcgctcgagc aggcgacctt gatgaccgac gacgccgtgc
     4021 tcgatgcgag ccacttcgcg gcacttgtct ccggtgcgga cggcacgcat acagcgaccc
     4081 caacggcaga cgcagccgcg attgcgccgc gcagcgcgcc ggtgacgcct gccgcgcagg
     4141 ctgcttcgaa cgacgaccgc tccgccgcga tcacgccgct gccgcaagcc atcgccgaac
     4201 tcgaggcgcg cacga
//
LOCUS       DATMJB010000679         2930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_784762, whole genome shotgun sequence.
ACCESSION   DATMJB010000679 DATMJB010000000
VERSION     DATMJB010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_784762"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1421
                     /locus_tag="VNU48_14380"
     CDS             <1..1421
                     /locus_tag="VNU48_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWI12517.1"
                     /translation="LVILVVLFVLQRHGTAGIGAVFGPVMVAWFVVLAGSGLVHLLRM
                     PAILAALNPLAGLAFALHHRWVAFVALGSVVLALTGAEALYADMGHFGAGPVRVSWFA
                     FVLPALTLNYFGQGALLLADPGALENPFFRMFPQWALVPMVLLATVATVIASQAVISG
                     AFSMTKQAIQLGFLPRMRILHTSDREIGQIYVPAVNTALLLAVIAAVVGFRSSTALGA
                     AYGIAVTGTMMITTFLTYYVLRYAWKFDARLAALATGFFFLVDLMFFGANLLKIVDGG
                     WFPLAIGAIVFIGMVTWARGRELLSESLRADAIELEPFMKGVLADPPMRAPGTAVFLN
                     AEADLTPTALLHNLKHNHVLHQTNLFVTVHTEEVPFDSRAVARELCQDCWTVTLHFGF
                     KNDQDVPAALELLRDRGIVLDPMTTSYFLSRSTIVPTPGPGMAPWREKLYANMHRNAA
                     SAADFLNLPPNRVVELGTKVQI"
     gene            1489..>2930
                     /locus_tag="VNU48_14385"
     CDS             1489..>2930
                     /locus_tag="VNU48_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HWI12518.1"
                     /translation="MSNNTSTSTGSSLAGLTLAAVGVVYGDIGTSPLYALKEVFAHGH
                     VILTAENIYGVLSLVFWTLTVVVSIKYVVLILRADNNGEGGLIAMLALASTAVRGKPG
                     LRRRLLLLGIFGTAIFFGDGVITPAITVLGAIEGLRVAAPGLDHYIVPVTLLVLTLLF
                     MVQRHGTANIGRFFGPVMVLWFAMLAVLGLLHIVENPAILWALSPHYALAFLVGNPHV
                     AFIALGSVVLCVTGAEALYADLGHFGKKPIRLAWFGLAMPALVLNYFGQGAMLLQHPG
                     RVRNPFFEMAPEWALYPLIGLATCAALIASQALITAAFSVTKQAIQLGYLPRLRILHT
                     SVREAGQIYVPFINWGLYVCIVIAVLVFGSTDRLAAAYGIAVTIDMLITTTMTFFVIR
                     YGWKYPWSLCFAATGFFFVVDFVYFSANVVKVFDGGWFPVAIGAVMFTLMMTWKQGRR
                     LMSARLREDAIDLKSFLEAVFVSPPTRVQGT"
BASE COUNT          382 a         1003 c          989 g          556 t
ORIGIN      
        1 cgctggtgat cctggtcgtg ctgttcgtgc tgcaaaggca cggcaccgcc gggatcggcg
       61 cggtgttcgg cccggtgatg gtggcctggt tcgtcgtgct ggccggatcc gggctggtcc
      121 atctgctgcg catgccggcg atcctggcgg cgctgaatcc gctggccggg ctggcgttcg
      181 cgctgcacca ccgctgggtc gctttcgtcg cgctcggctc ggtcgtgctg gcgctgaccg
      241 gcgccgaggc gctgtacgcc gacatgggtc acttcggcgc cgggccggtg cgggtgtcgt
      301 ggttcgcgtt cgtgctgccg gcgctgaccc tgaactactt cggccagggc gcgctgctgc
      361 tggccgaccc gggcgcgctc gagaacccgt tcttccgcat gttcccgcag tgggcgctgg
      421 tgccgatggt gctgctggcc accgtggcca ccgtgatcgc gtcgcaggcg gtgatctcgg
      481 gcgcattctc gatgaccaag caggcgatcc agctcggctt cctgccgcgc atgcgcatcc
      541 tgcacacctc ggaccgcgag atcggtcaga tctacgtgcc ggcggtcaac accgcgctgc
      601 tgctcgcggt gatcgctgcg gtcgtcggct tccgctcgtc gacggcgctc ggcgcggcct
      661 acggcatcgc ggtcaccggc acgatgatga tcacgacctt cctgacctac tacgtgctgc
      721 gctacgcgtg gaagttcgac gcccggctgg ccgcgctcgc gaccggcttc ttcttcctcg
      781 tcgacctgat gttcttcggt gcgaacctgc tgaagatcgt cgacggcggc tggtttccgc
      841 tggccatcgg cgccatcgtc ttcatcggca tggtgacctg ggcgcgcggc cgcgagctgc
      901 tgtcggaaag cctgcgcgcc gacgcgatcg aactcgaacc ctttatgaag ggcgtgcttg
      961 ccgatccacc gatgcgcgcg ccgggcaccg cggtgttcct gaacgccgag gccgatctga
     1021 cgccgacggc gctgctgcac aacctgaagc acaaccacgt gctgcatcag accaatctgt
     1081 tcgtcaccgt gcacaccgag gaagtgccgt tcgacagtcg ggccgtcgcg cgcgagctgt
     1141 gtcaggactg ctggaccgtc acgctgcatt tcggcttcaa gaacgaccag gacgtgccgg
     1201 ccgcgctcga gctgctgcgc gatcgcggca tcgtgctcga cccgatgacg acctcgtact
     1261 tcctgtcgcg ctcgacgatc gtgccgacgc cggggcccgg catggcgccg tggcgcgaga
     1321 agctctacgc caacatgcat cgcaacgcgg cttcggccgc ggacttcctg aacctgccgc
     1381 cgaaccgcgt cgtcgaactc ggcacgaagg tccagatctg agcctgagcc gcggcctgcg
     1441 cgcgactgcg ggcacaatgc ccgctccccg ccgtcgcgca cgcccgagtt gagcaacaac
     1501 acctccacgt ccaccggatc aagtctcgcc ggtctcaccc tggctgccgt cggcgtcgtc
     1561 tacggcgaca tcggcaccag cccgctgtac gcgctgaagg aggtgttcgc gcacggccac
     1621 gtgatcctga cggccgagaa catctacggc gtgctgtcgc tggtgttctg gaccctgacc
     1681 gtcgttgtct cgatcaagta cgtggtgctg atcctgcgcg ccgacaacaa cggcgagggc
     1741 ggcctgatcg cgatgctggc gctggcctcg acggcggtgc gcggcaagcc cgggctgcgc
     1801 cggcgcctgc tgctgctggg catcttcggc accgcgatct tcttcggcga cggcgtgatc
     1861 acgccggcca tcaccgtgct cggcgcgatc gagggcctgc gggttgccgc gccgggcctg
     1921 gaccactaca tcgtgccggt cacgctgctc gtgctgacgc tgctgttcat ggtgcagcgc
     1981 cacggcacgg cgaacatcgg ccgcttcttc ggcccggtga tggtgctgtg gttcgccatg
     2041 ctggcggtgc tgggcctgct gcacatcgtc gagaacccgg cgatcctgtg ggcgctgagc
     2101 ccgcattacg cgctggcgtt cctggtcggc aacccgcacg tggccttcat cgcgctcggc
     2161 tcggtcgtgc tgtgcgtgac cggcgccgag gcgctgtatg ccgacctggg ccacttcggc
     2221 aagaagccga tccggctggc ctggttcggc ctggcgatgc cggcgctggt gctgaactac
     2281 ttcggccagg gcgcgatgct gctgcagcac ccgggccgtg tgaggaaccc gttcttcgag
     2341 atggcacccg agtgggcgct gtacccgctg atcggcctgg cgacctgtgc cgcgttgatc
     2401 gcctcgcagg cgctgatcac cgcggcgttc tcggtcacga agcaggcgat ccagctcggc
     2461 tacctgccgc ggctgcgcat cctgcacacc tcggtgcgcg aggccgggca gatctacgtg
     2521 cccttcatca actggggcct gtacgtgtgc atcgtgatcg cggtgctggt gttcggctcg
     2581 accgaccggc tcgcggcggc ctacggcatc gcggtgacga tcgacatgct gatcaccacc
     2641 acgatgacct tcttcgtgat ccgctacggc tggaagtacc cgtggtcgct gtgcttcgcg
     2701 gcgaccggct tcttcttcgt cgtcgacttc gtctacttca gcgcgaacgt cgtcaaggtg
     2761 ttcgacggcg gctggttccc ggtcgcgatc ggcgcggtga tgttcacgct gatgatgacc
     2821 tggaagcagg gccgccggct gatgtcggcg cgcctgcgcg aagacgcgat cgacctgaag
     2881 agttttctcg aagccgtgtt cgtgagcccg cccacgcgcg tgcagggcac
//
LOCUS       DATMJB010000680         2339 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_804172, whole genome shotgun sequence.
ACCESSION   DATMJB010000680 DATMJB010000000
VERSION     DATMJB010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_804172"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VNU48_14390"
     CDS             <1..415
                     /locus_tag="VNU48_14390"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HWI12519.1"
                     /translation="VRAACHGLALKVILETGVLVDDALIARAAQLSLDAGADFLKTST
                     GKTPVSATPQAARVMLAAIAADPRAAARAGFKASGGIRSVTDAAVYLDLCERALGREA
                     LRPARFRIGASSLLNDIEAVLAGTPAAPRSTPGTY"
     gene            425..1753
                     /gene="deoA"
                     /locus_tag="VNU48_14395"
     CDS             425..1753
                     /gene="deoA"
                     /locus_tag="VNU48_14395"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HWI12520.1"
                     /translation="MLAQEIIRRKRDGQALDRAQIDAFVAGLVDASWSEAQVAAMAMA
                     MFMNGLSHAETIALTHAMTRSGDVLDWADAKLGGPVLDKHSTGGVGDKVSLLLAPIVA
                     ACGGFVPMISGRGLGHTGGTLDKLDSIAGYCTAASGAQLRAALRSAGCAIVGQTATLA
                     PADRRLYAIRDVTATVESIPLITASILSKKLAAGLQGLVIDVKLGNGAFATGLPMARA
                     LARSLVQVAGGAGLPTRACLSDMNEVLGHSCGNAVEVHEALAFLQGTRREPRLLELTR
                     RLSAELVLLGGLASHMDGALARVDAVLADGRALERFARMVAALGGPLDFVERAAGYLP
                     AAPAQRVLRAPRAGWVGAMATREIGLLVVELGGGRRQASDAVDHRVGLSEIVAIGRRV
                     AAGDALAIVHAADEASAAAGCERLAALIEIADAAPAPRAVVIECTDEVAP"
     gene            1750..>2339
                     /locus_tag="VNU48_14400"
     CDS             1750..>2339
                     /locus_tag="VNU48_14400"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWI12521.1"
                     /translation="MTYALAAGPDVVVVGSLNLDLVVRAPRLPRPGETLIGSDFATDE
                     GGKGANQAVAAARMGARVAMIGRLGRDAHGARLRAALQLEGVDDAGVGDDDARPTGVA
                     SIVVATGGENCIVVVPGANHALQPRHVEACAALLAAARIAVLQFEIPMPAVIAALQHA
                     RTGGATTLLNAAPAADLATTTFALVDWLVVNQEEAG"
BASE COUNT          301 a          818 c          908 g          312 t
ORIGIN      
        1 ggtgcgcgcg gcgtgtcacg gcctggcgct gaaggtgatc ctcgagaccg gcgtgctggt
       61 cgacgacgcg ctgatcgcgc gcgccgcgca gctgagcctg gacgccggcg ccgacttcct
      121 gaagaccagc acgggcaaga cgccggtcag cgcgacgccg caggcggcac gcgtgatgct
      181 ggcggcgatc gccgccgatc cgcgcgccgc cgcacgcgcc ggcttcaagg cttcgggcgg
      241 catccgcagc gtgaccgatg cggcggtcta ccttgatctg tgcgagcggg cgctgggccg
      301 cgaggcgctg cggccggcgc gctttcggat cggcgcgagc agcctgctga acgacatcga
      361 agcggtgctc gccggaacgc ccgcagcgcc acggtcgacg ccgggaacct actgaggcca
      421 cgcggtgctc gctcaggaaa tcatccgccg caaacgcgac ggccaggcgc tggaccgggc
      481 gcagatcgac gcgttcgtcg ccggcctcgt cgatgcctcg tggagcgagg cgcaggtcgc
      541 cgcgatggcg atggcgatgt tcatgaacgg cctgtcgcat gccgagacca tcgcgctgac
      601 gcatgcgatg acgcgctccg gcgacgtgct cgactgggcc gatgcgaagc tcggcggccc
      661 ggtgctcgac aagcactcga ccggcggcgt cggcgacaag gtcagcctgc tgctcgcgcc
      721 gatcgtcgcg gcctgcggcg gcttcgtacc gatgatctcg ggccgcggcc tgggacacac
      781 cggcggcacg ctcgacaagc tcgacagcat tgccggctac tgcacggcgg catcgggtgc
      841 gcaattgcgc gctgcgctgc gcagtgccgg ctgcgcgatc gtcgggcaga cggccacgct
      901 ggcgccggcc gaccgacgcc tgtatgcgat ccgcgacgtg accgcgacgg tcgagtcgat
      961 accgctgatc accgcgagca tcctgtcgaa gaaactcgcc gccgggctgc aggggctggt
     1021 gatcgacgtg aagctcggca acggcgcgtt cgcgaccggg ctgccaatgg cgcgcgcgct
     1081 cgcgcgctcg ctggtgcagg tcgccggcgg cgcgggcctg cccacacgcg cctgcctcag
     1141 cgacatgaac gaggtgctcg gccacagctg tggcaacgcg gtcgaggtgc acgaggcgct
     1201 ggccttcctg cagggcacgc ggcgcgagcc gcgcctgctc gaactcacgc gccggctcag
     1261 cgccgaactg gtgctgctcg gcgggcttgc gagccacatg gacggggcgc tcgcgcgtgt
     1321 cgacgcggtg ctcgcggacg gccgcgcgct cgagcgcttc gcgcgcatgg tcgcggcgct
     1381 cggcggcccg ctggatttcg tcgagcgtgc cgcgggctac ctgccggccg cgccggcgca
     1441 gcgcgtgttg cgcgcgccgc gcgccggctg ggtcggcgcg atggcgacgc gcgagatcgg
     1501 gctgctcgtg gtcgagctcg gcggcggtcg gcgccaggcc agcgacgcgg tggatcatcg
     1561 cgtgggcttg agcgagatcg tggcgatcgg gcggcgcgta gctgccggcg atgcgctggc
     1621 gatcgtgcac gctgccgatg aggcgtccgc ggcggccggc tgcgaacgcc tcgcggcgct
     1681 gatcgagatc gcggacgccg cgccggcgcc gcgcgcggtg gtgatcgagt gcaccgacga
     1741 ggtcgcgcca tgacgtatgc gctggccgcc ggcccggacg tggtcgtcgt cggcagtctg
     1801 aacctcgacc tggtggtgcg cgcgccgcgc ctgccgcggc ctggcgagac gctgatcggc
     1861 agcgacttcg cgaccgacga aggcggcaag ggcgcgaacc aggcggtcgc ggcggcgcgc
     1921 atgggcgcgc gcgtcgcgat gatcggccgc ctcggccgcg atgcgcacgg cgcgcggctg
     1981 cgcgcggcgc tgcaactcga gggcgtggac gacgccggtg tcggcgacga cgacgctcgg
     2041 ccgaccggcg tggcgtccat cgtcgtcgcc accggcggcg agaactgcat cgtcgtcgtg
     2101 cccggcgcga accatgcgct gcagccgcgg catgtcgagg catgcgccgc gctgctggcg
     2161 gcggcacgga tcgccgtgct gcagttcgag attccgatgc cggccgtgat cgcggcgctg
     2221 cagcacgcgc gcacgggcgg cgcgacgacc ctgctgaatg cggcgccggc cgccgatctc
     2281 gcgacgacga cattcgcgct ggtcgattgg ctcgtcgtca accaggaaga ggccggcag
//
LOCUS       DATMJB010000681         2876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_672396, whole genome shotgun sequence.
ACCESSION   DATMJB010000681 DATMJB010000000
VERSION     DATMJB010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672396"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VNU48_14405"
     CDS             complement(<1..807)
                     /locus_tag="VNU48_14405"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HWI12522.1"
                     /translation="MKFEILPAANPTSDKDRAAKLVDPGFGRVFTDHMAIVRYNQANG
                     WHGARVEARANFPVDPALAVLHYAQEIFEGLKAYARDDGGVNLFRPDANARRFRTSAD
                     RMAMAQLPEPVFIEAVEQLVRIDRAWIPGGEGSLYLRPFMIASEVFLGVKPSAEYIFA
                     VIASPVGSYFKGGPAPVSIWVSEKYTRAAIGGTGAVKCGGNYAASLRAQAEAIEHGCD
                     QVVFLDAVQRKYVEELGGMNVFFVFEDGSLLTPPLGTILPGITRDSIIQLA"
     gene            1136..1729
                     /locus_tag="VNU48_14410"
     CDS             1136..1729
                     /locus_tag="VNU48_14410"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HWI12523.1"
                     /translation="MIDSFVTLNLRYVNMADINFSGSPTGPDSRAGGARPAPRSGDLR
                     WDIIELLFFAYRDFVGDADHELEAFGFGRAHHRVMHFVHRYPGLKVADLLDVLRITKQ
                     SLGRVLKQLLDEGYIVQKTGDNDRRQRLLFATAKGETMVARLAGLQTDRINRALADIG
                     PAGADTVRQFLRAMIDHDDPDKVLETILAAGSATAKE"
     gene            1726..2466
                     /locus_tag="VNU48_14415"
     CDS             1726..2466
                     /locus_tag="VNU48_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWI12524.1"
                     /translation="MIVPQGAVSTATVPRPPPRPADDAPHLLLVDDDRRIRDLLSRFL
                     CGEGYRVTTAMSASDARAKLLGLHFDLLILDVMMPGETGFDLARFIRTSSSVPIIMLT
                     ARHEAEARIEGLQIGADDYVAKPFEPRELVLRIGNILKRAAPAPVETLEQVVFGPYVF
                     HLERGELRQGDETIHLTDREREMLRILALARGETVPRNTLTGNGTVNERAVDVQINRL
                     RRKIERDPANPLFLQAVRGVGYRLVASP"
     gene            2491..>2876
                     /locus_tag="VNU48_14420"
     CDS             2491..>2876
                     /locus_tag="VNU48_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12525.1"
                     /translation="MSTLDTGLTLIRTAAGRVSAANGRMGNMFKGWMPTGLYARALLI
                     MIVPMVMLQTVVAFVFMERHWNTVTRHLSAAVVQDIAGVIDIYKTFPQDKDRTQLKRI
                     AQERLGLVVDFLPRGDMPSPGPKPFFS"
BASE COUNT          500 a          948 c          913 g          515 t
ORIGIN      
        1 ggcaagctgg atgatggagt cgcgggtgat gcccggcagg atcgtgccga gcggcggcgt
       61 caagagcgaa ccgtcctcga acacgaagaa cacgttcatg ccgccgagct cttcgacgta
      121 tttgcgctgg accgcgtcga ggaacacgac ctggtcgcag ccgtgctcga tggcttccgc
      181 ctgcgcgcgc aggctcgcgg cataattgcc gccgcacttg acggcgccgg tgccgccgat
      241 cgcggcgcgc gtgtactttt ctgacaccca gatcgacacc ggcgcgggtc cgcccttgaa
      301 ataggagccg accggcgagg cgatgacggc gaagatgtat tccgctgacg gtttcacgcc
      361 gaggaagact tcgcttgcga tcatgaaggg ccgcaaataa aggctgccct cgccgcccgg
      421 gatccaggcg cggtcgatgc gcacgagttg ctcgaccgcc tcgatgaaca cgggctcggg
      481 cagctgcgcc atggccatac ggtcagccga agtccggaag cgccgcgcat tggcgtcggg
      541 gcggaacagg ttcacgccgc cgtcgtcgcg ggcgtaggcc ttgaggcctt cgaaaatctc
      601 ctgtgcgtag tgcagcacgg ccaaggccgg atcgaccgga aaattcgcac gcgcttcgac
      661 gcgcgcgccg tgccagccat tcgcctgatt gtagcggacg atggccatgt gatcggtgaa
      721 aacgcgaccg aagccggggt ccacgagctt tgccgcgcgg tccttgtcgg acgtcggatt
      781 ggccgcgggc aggatctcga atttcaaact cgtgcccttg ctgtccggcg ccggcctgtc
      841 tctacgggcc tgctctagcg aaactgccat ggttctctcc cggattgatg cagatccatg
      901 cctgcaccct ggcgctggtt atgacgcctg gccttcccgc gcaccagcct cgcggccggt
      961 ttccgcgccg gcttgccccg aggacatgct tttgcggaat gcggaagtcc agtatgtttg
     1021 ccgaaatgcc gctcgacaat ctcaacggta gacgaattcg cggctcaaac agccatctct
     1081 ggcttactcg ggtcgctggt gagtcgctgc ggacgaaacg aactaatatt tcgtcgtgat
     1141 cgactctttt gtaacattga acctaagata tgtcaatatg gctgacataa atttctccgg
     1201 ctcccccacc ggccctgatt caagggctgg cggcgcgcgc cctgccccac gttcgggcga
     1261 cctgcgctgg gacatcatcg agctgttgtt tttcgcctac cgggacttcg tcggcgatgc
     1321 cgaccacgag ctggaggctt tcggcttcgg acgcgcgcat caccgggtga tgcatttcgt
     1381 ccatcgctat cccggcctca aggtggccga cctgctggac gtgctgcgca tcaccaagca
     1441 gtcgctcgga cgggtgctca aacagctgct cgacgaaggc tatatcgtgc agaagaccgg
     1501 cgataacgac cgccggcagc gtcttctttt cgccaccgcc aagggtgaga cgatggtggc
     1561 cagactcgca gggctgcaga ccgaccggat caaccgcgcg cttgccgaca tcggcccggc
     1621 aggcgcggat acggtccggc agttcctgcg cgcgatgatc gatcacgacg atcccgacaa
     1681 ggtgctggaa acgatcctcg ccgccggcag cgcaaccgca aaggaatgat cgtgccccag
     1741 ggagccgtaa gcaccgcaac cgtcccgcgc ccgccgccgc ggccggccga tgacgccccg
     1801 catctgctgc tggttgatga cgaccgccgc atccgcgacc tgttgtcgcg ctttctgtgc
     1861 ggcgaaggct atcgcgtcac cacggcgatg agcgccagcg acgcgcgcgc caagctgctc
     1921 ggcctgcatt tcgacctgct gatcctcgac gtgatgatgc ccggcgaaac cgggttcgac
     1981 ctggcgcgct tcatccgcac ctcgtcctcg gttccgatca tcatgctgac ggcgcgccat
     2041 gaggccgaag cccggatcga aggcctgcaa atcggcgccg acgattacgt cgccaaaccg
     2101 ttcgagccgc gcgagctggt gcttcggatc ggcaatatcc tcaagcgcgc ggcgccggcg
     2161 ccggtggaga cgctggagca ggtcgtattc ggaccctacg tcttccatct ggagcgcggc
     2221 gagctgcgcc agggcgacga gaccattcac ctcaccgatc gcgagcgcga gatgctgcgc
     2281 attctggcgc tggcgcgcgg cgagaccgtg ccgcgcaata cgctgaccgg caacggcacc
     2341 gtcaacgagc gcgcggtcga cgttcaaatc aaccgcctgc ggcgcaagat cgagcgcgat
     2401 ccggccaatc cgctgttcct gcaggcggtg cgcggcgtcg gctatcgcct cgtcgcgtcg
     2461 ccctgagccg tcacggatga attcagcccg atgagcacgc tcgacaccgg cctcacgctg
     2521 atccgcaccg ctgccggccg tgtgtccgcg gccaatggcc ggatgggcaa catgttcaag
     2581 ggctggatgc cgaccggcct ctacgcccgc gcgctgctga tcatgatcgt gccgatggtg
     2641 atgctgcaga cggtggtggc gttcgttttc atggagcggc actggaacac ggtgacaagg
     2701 catttgtcgg ccgccgtggt gcaggatatc gccggcgtga tcgacatcta caagactttc
     2761 ccgcaggaca aagaccgcac gcagctcaag cgcatcgcgc aggaacggct gggactcgtg
     2821 gtcgatttcc ttccccgggg cgacatgccc tcgccggggc cgaagccctt cttctc
//
LOCUS       DATMJB010000682         2188 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_91755, whole genome shotgun sequence.
ACCESSION   DATMJB010000682 DATMJB010000000
VERSION     DATMJB010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91755"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..738)
                     /gene="pncA"
                     /locus_tag="VNU48_14425"
     CDS             complement(22..738)
                     /gene="pncA"
                     /locus_tag="VNU48_14425"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HWI12526.1"
                     /translation="MHTTTRRTLLKALSGAAALAASGASFAAAKLAPDATSALIVVDV
                     QNCFVTGGTLPVKGGEEVVPVINRIAASFQNIVVTQDWHTPGHASFASTYQGKKPFET
                     TKLSYGTQVLWPDHCVQGTDDAALHKDLKLPTAQIIIRKGFHKDMDSYSAFDEADHKT
                     ATGLAGYLKARGIKTLYITGLATDFCVAWTAMDARKAGFEVYVIEDATRAIDLNGSLA
                     AAWKGMTAKGVKRIQSSDLA"
     gene            complement(803..1534)
                     /locus_tag="VNU48_14430"
     CDS             complement(803..1534)
                     /locus_tag="VNU48_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI12527.1"
                     /translation="MNTTELLDAKALCAWYGAAQILYDVDLQVRRGEVVALMGRNGAG
                     KSTTLKALIGLLAKRRGTVRFLGEDVSKAEPYEVARRGLGYVPEDRRVFGDLTVLENL
                     EVGRQPARQWADGSAAPLWTTDKLFKLFPNLGEMPDRPGGRMSGGEQQMLTVARTLMG
                     NPYLVLLDEPSEGVAPVIVEQMVQMILALKAQGVSILLSEQNMHFAELVSDRAYVLEK
                     GQIRYQASMAELAVNEEVRRAYLSV"
     gene            complement(1531..>2188)
                     /locus_tag="VNU48_14435"
     CDS             complement(1531..>2188)
                     /locus_tag="VNU48_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI12528.1"
                     /translation="AGKTTTFNMVNGQLRADTGSVRLAGTELIGRKPRDIWHRGVGRT
                     FQIAETFASLTVVENVQMALLSADAKLYSLWKRAADHRRDDALALLAQVGMQAQADRP
                     CSVLAYGDVKRVELAIAMANAPKLLLMDEPTAGMAPKERNDLMALTKKLVVEKGLAVL
                     FTEHSMDVVFAYADRIIVLARGRLIAEGKPAEIRDHPRVQEVYFGSGATFETKKAALA
                     "
BASE COUNT          315 a          750 c          728 g          395 t
ORIGIN      
        1 gtcagcgcga tcacggccgg gtcaggccag gtcgctcgac tggatgcgct tcacgccctt
       61 cgcggtcatg cccttccagg ccgcggcgag cgagccgttc aggtcgatcg cgcgcgtcgc
      121 gtcctcgatc acgtagacct cgaagccggc cttgcgcgcg tccatcgcgg tccaggcgac
      181 gcagaagtcg gtcgccagcc cggtgatgta cagcgtcttg atgccgcgcg ccttcaggta
      241 gccggccaga cccgtggcgg tcttgtgatc ggcttcgtcg aacgcggagt agctgtccat
      301 gtccttgtga aagcctttgc ggatgatgat ctgcgcggtc ggcagcttca ggtccttgtg
      361 cagcgccgcg tcgtcggtgc cctgcacgca gtggtcgggc cacagcacct gcgtgccgta
      421 gctcagcttg gtggtctcga acggcttctt gccttgatac gtgctcgcga acgacgcgtg
      481 gcccggcgtg tgccagtcct gcgtgacgac gatgttctgg aagctggcgg ctattctgtt
      541 gatcaccggc acgacctctt cgcccccctt caccggcaag gtgccgccgg tgacgaagca
      601 gttctgcacg tcgacgacga tcagcgcgct cgtcgcatcg ggcgcgagct tcgcggcggc
      661 gaacgacgcg ccgctcgcgg cgagcgcggc ggcgccggac agtgccttca acagggttct
      721 gcgggtggtg gtatgcatgg cgaggacctc ctgatgatga gtgtgtgagc ctgtgtcggc
      781 ggcgcgtcgc agaccgagcc ttctagacgc tgagataggc gcgccgcact tcctcgttga
      841 ccgcaagctc ggccatcgaa gcctggtagc ggatctggcc cttctcgagc acgtaggccc
      901 ggtcggacac cagctccgcg aaatgcatgt tctgctcgga cagcaggatg ctgacaccct
      961 gcgccttcag cgccaggatc atctgcacca tctgctcgac gatcaccggc gcgacgccct
     1021 cgctcggctc gtccagcagc acgagatagg ggttgcccat cagtgtgcgc gcgaccgtca
     1081 gcatctgctg ctcgccgccg ctcatgcggc cgccggggcg gtcgggcatc tcgccgagat
     1141 tggggaacag cttgaacagc ttgtcggtcg tccacagcgg cgccgcgctg ccgtcagccc
     1201 attgtctggc gggctggcgg ccgacctcga ggttctcgag caccgtcagg tcgccgaaca
     1261 cgcgacggtc ctcgggcacg tagcccaggc cgcggcgcgc gacctcgtat ggctcggcct
     1321 tcgacacatc ctcgccgaga aagcgcaccg tgccgcggcg cttcgcgagc agcccgatca
     1381 gcgccttcag cgtggtcgac ttgccggcac cgttgcggcc catcagcgcg acgacttcgc
     1441 cgcgtcgcac ctgcagatcg acgtcgtaga ggatctgcgc ggcgccgtac catgcgcaca
     1501 gcgccttcgc gtcgagcagc tcggtggtgt tcatgccagc gccgccttct tcgtttcgaa
     1561 ggtggcgccg ctgccgaaat agacttcctg gactctcggg tgatcgcgga tctcggccgg
     1621 cttgccctcg gcgatcaggc ggccccgcgc cagcacgatg atccggtcgg cgtacgcgaa
     1681 caccacgtcc atgctgtgct cggtgaacag caccgccaaa cccttctcga cgacgagctt
     1741 cttggtcagc gccatcaggt cgttgcgttc cttcggcgcc atgccggcgg tgggctcgtc
     1801 catcagcagc agcttcgggg cgttcgccat cgcgatcgcg agctcgacgc gcttgacgtc
     1861 gccgtaggcg agcacgctgc acggccgatc ggcctgcgcc tgcatgccga cctgggccag
     1921 cagcgcgagc gcatcgtcgc ggcgatggtc ggccgcgcgc ttccacagcg aatacagctt
     1981 cgcatcggcc gagagcagcg ccatctgcac gttctcgacc acggtcagcg aggcaaaggt
     2041 ctcggcgatc tggaaggtgc ggccgacgcc gcggtgccag atgtcgcgcg gcttgcggcc
     2101 gatcagctcg gtgccggcca gcctcaccga gccggtgtcg gcgcgcagct ggccgttgac
     2161 catgttgaac gtggtcgtct tgccagcg
//
LOCUS       DATMJB010000683         4544 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_32761, whole genome shotgun sequence.
ACCESSION   DATMJB010000683 DATMJB010000000
VERSION     DATMJB010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32761"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VNU48_14440"
     CDS             <1..322
                     /locus_tag="VNU48_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012426272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWI12529.1"
                     /translation="LKPSARRFIESAEQVFVSAASIWEIAIKARLGKIDADPQELVAA
                     IGTSGFSELPVRAAHAVEVTRLALHHNDPFDRLLVAQAISEPLKLLTADAVMSQYGDF
                     VVMA"
     gene            432..1415
                     /locus_tag="VNU48_14445"
     CDS             432..1415
                     /locus_tag="VNU48_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI12530.1"
                     /translation="MLVFVLRRLAQAVIVMLAVAFIAFMLFQYVGDPVNNILGQDATP
                     EQRAQLRADLGLDKPFPVQFARFVGNAAQGRFGLSLRQGRMVSSLIAERLPATLELSF
                     SAAIIALVLGVPMGVYAALRRGTFLSQTLMTLSLLGVSLPVFLIGILLILVFAVTLKW
                     LPSFGRGEVTMLGTWSTGFLTVDGWKHLILPSITLAVFQLALITRLVRSEMLEVLRAD
                     YIKFARARGLPNRTVYFGHALKNTLVPVMTITGLQLGGLIAFAIITETVFQWPGMGLL
                     FIQAVTFADIPVMAAYLCLIALIFVVINLAVDMLYFVVDPRLRAEKSTAGH"
     gene            1468..3045
                     /locus_tag="VNU48_14450"
     CDS             1468..3045
                     /locus_tag="VNU48_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI12531.1"
                     /translation="MKFKKRALLTASLVLACGLSSAVTLRVANQGDVQSMDPHSLNES
                     LQLAFGNSIYESLVGRGKDMSVVPLLATKWRQTSPTVWRFDLRKGVKFHDGTPFTADD
                     VIFSFKRATDAGSDMKGYVAPIKELRKIDDHTVDIETVTPFPILPDTLTVFAIMSKKW
                     CEDNKAEKPVDRRKGIENTASFKANGTGPFRLKERQPSVRTVLARNFNYWDKVEGNVD
                     EVVFTPIGSDATRVAALLSGEIDVMEPVPLQDIERIKASSGATVLQGPELRTIFLGMD
                     QKRDELLFSSVKGKNPFKDKRVRQAFYQAIDVETIKSRVMRGAATPTALMVGPGVNGF
                     QADMNKRLPYDVEAAKKLMADAGYASGFEVAMNCPNDRYVNDAAICQAVAANLARIGI
                     KINLQTETKNTYFPKILRRDTSLYLLGWTPSTTDAHDALYNLMATPTDKGQGQYNLGA
                     YSNPKLDELTAKVQIETDKAKRDQMIREAFKVHLDDVGHIPLHQQALAWAYAKNVTLT
                     QLPNNYMFFKWISVKGK"
     gene            3057..3992
                     /locus_tag="VNU48_14455"
     CDS             3057..3992
                     /locus_tag="VNU48_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI12532.1"
                     /translation="MSSVIDTPVPGAWARFLDGDVWHSFRSSPMAIGAAAIALSCVFL
                     ALFANLIAPHNPFDLATLELSDSMLPPAWMHGGQGKYLLGTDEQGRDVLSALMFGARI
                     SLLIGGASVIVSVLIGVSLGLLSGYAGGRTDAFIMRVCDVMLSFPSILIALLIAGVGR
                     ALFPQAHDSLAFGVLIIAIALPGWVQYARTVRGSTMVERSKEYVQAARVVGVPPLRIM
                     LRHVLPNVLGPVLVLATIHLALAIQTEATLSFLGVGVSPTSPSLGTFINEGNKQLFSG
                     AWWLVIFPGAMLLLIALSMNLFGDWLRDALNPRLR"
     gene            4014..>4544
                     /locus_tag="VNU48_14460"
     CDS             4014..>4544
                     /locus_tag="VNU48_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWI12533.1"
                     /translation="MTTPLLEVRNLRVEFPTRRGKLVALDDISFDIAPGEILGVVGES
                     GAGKSLTGAAIIGLLDPPGHVAAGEIRFDGRRIDNLPYEEMRKVRGRQIGAIFQDPLT
                     SLNPLYTVGRQLIETIRTHLPVSADEARRRAIKLLQETGIPAAQARIDQYPHQFSGGM
                     RQRVVIALALAAEPKLI"
BASE COUNT          751 a         1522 c         1470 g          801 t
ORIGIN      
        1 cctcaaacct tcggcccggc ggttcatcga atcggcggag caagtcttcg tctcggccgc
       61 ctcgatctgg gaaatcgcca tcaaggctcg gctcggcaag atcgacgccg acccgcagga
      121 actggtcgct gccatcggga cgagcggatt ttcggagctc ccggtacgtg ccgcgcacgc
      181 ggtcgaggtg acgcgactcg cgcttcatca caacgatccc tttgaccgcc tgctggttgc
      241 gcaagcgatc agcgagccgc tgaaattgtt gaccgccgac gccgtgatga gccagtacgg
      301 cgacttcgtg gtcatggcct gatcgacgcg caaccggcgc gattgctcgg tcaggtgctt
      361 gctcaccggg agtcagcacc atcggggcat tcccctttga cgagcggttc acgtcttgct
      421 ttcatcgatc aatgcttgtt ttcgttctcc gccgcctcgc gcaagccgtg atcgtgatgc
      481 tcgcggtcgc gttcatcgcg ttcatgctgt tccagtatgt cggcgacccg gtgaacaaca
      541 tcctcggcca ggacgcgacg cccgagcagc gcgcccaact gcgcgccgac ctcggcctcg
      601 acaagccgtt cccggtgcag ttcgcgcgct tcgtcggcaa tgccgcgcag ggccggttcg
      661 gcctgagcct gcgacagggt cgcatggtgt cgtcgctgat cgccgagcgt ctgccggcca
      721 cgctcgaact gtcgttctcg gccgccatca tcgcgctggt gctcggcgtc ccgatgggcg
      781 tctacgcggc gctgcggcgc ggcacgttcc tgtcgcagac gctgatgacc ttgtcgttgc
      841 tgggtgtctc gctaccggtc ttcctgatcg gcatcctgct gatcctggtg ttcgcggtca
      901 cgctgaaatg gctgcccagc ttcggccgcg gcgaggtcac gatgctcggc acctggagca
      961 ccggctttct gacggtcgac ggctggaagc atctgatcct gccgtcgatc acgctggcgg
     1021 tgttccagct ggcgctgatc acgcggctgg tgcgctccga gatgctcgag gtgctgcgcg
     1081 ccgactacat caagttcgcg cgcgcgcgcg gcctgccgaa tcgcacggtc tatttcggcc
     1141 acgcgctgaa gaacacgctg gtgccggtga tgacgatcac cgggctgcaa ctcggcggcc
     1201 tgatcgcgtt cgcgatcatc accgagacgg tgttccaatg gcccggcatg gggctgctgt
     1261 tcatccaggc cgtcaccttc gccgacattc cggtgatggc ggcgtatctg tgcctgatcg
     1321 cgctgatctt cgtcgtgatc aacctggcgg tcgacatgct ctacttcgtc gtcgacccgc
     1381 gcctgcgcgc cgagaagtcc acggcagggc attgagttcg cagtgctcca atcccgctgt
     1441 cgatcgtcac cgtcgttgga gacattcatg aagttcaaga aaagagccct tctcaccgcc
     1501 tcgctcgtgc tggcctgcgg gctgtccagc gcggtcacgc tgcgcgtggc caatcagggc
     1561 gacgtgcagt cgatggaccc gcactcgctg aacgagtcgc tccagctcgc cttcggcaac
     1621 agcatctacg agtcgctcgt cgggcgcggc aaggacatgt cggtggtgcc gctgctcgcc
     1681 accaagtggc gccagacctc gccgacggtg tggcgcttcg acctgcgcaa gggcgtgaaa
     1741 ttccacgatg gcacgccgtt cacggccgac gacgtgatct tcagcttcaa gcgcgcgacc
     1801 gacgcgggca gcgacatgaa gggctacgtc gcaccgatca aggaactgcg caagatcgac
     1861 gatcacaccg tcgacatcga gaccgtgacg ccgttcccga tcctgcccga cacgctcacc
     1921 gtgttcgcga tcatgagcaa gaaatggtgc gaggacaaca aggccgagaa gccggtcgat
     1981 cgccgcaagg gcatcgagaa caccgccagc ttcaaggcca acggcaccgg cccgttccgc
     2041 ctgaaggagc gccagccctc ggttcgcacg gtgctcgcgc gcaacttcaa ctactgggac
     2101 aaggtcgagg gcaacgtcga cgaagtggtg ttcacgccga tcggcagcga cgccacccgt
     2161 gtggccgcgc tgctgtcggg cgagatcgac gtgatggagc cggtgccgct gcaggacatc
     2221 gagcgcatca aggcctcgag cggcgccacc gtgctgcaag gccccgagct gcgcacgatc
     2281 tttctcggca tggaccagaa gcgcgacgag ctgctgttca gcagcgtcaa gggcaagaac
     2341 ccgttcaagg acaagcgcgt gcgccaggcg ttctaccagg cgatcgatgt cgagacgatc
     2401 aagtcgcgcg tgatgcgcgg cgcggccacg ccgaccgcgc tgatggtcgg cccgggcgtg
     2461 aacggcttcc aggccgacat gaacaagcgc ctgccgtatg acgtcgaggc cgcgaagaag
     2521 ctgatggccg acgccggcta cgcgagcggt ttcgaggttg cgatgaattg ccccaacgac
     2581 cgctacgtca acgacgccgc gatctgtcag gcggtggccg ccaacctggc gcgcatcggc
     2641 atcaagatca acctgcagac ggaaaccaag aacacctatt tcccgaagat cctgcgccgc
     2701 gacacgagct tgtacctgct cggctggacg ccgagcacca ccgacgccca tgacgcgctg
     2761 tacaacctga tggccacgcc gaccgacaag ggccagggcc agtacaacct gggcgcgtac
     2821 agcaacccca agctcgacga gctcacggcc aaggtccaga tcgaaaccga caaggccaag
     2881 cgcgaccaga tgatccgaga agcgttcaag gtccatctcg acgacgtggg ccacataccg
     2941 ctgcaccagc aggcgctggc ctgggcctac gccaagaacg tgacgctgac gcaactgccc
     3001 aacaactaca tgttcttcaa gtggatctcg gtgaagggca agtgaaggat caatcggtga
     3061 gctcggtgat cgacacgccc gtgcccggcg cctgggcgcg ctttctcgac ggcgatgtct
     3121 ggcactcgtt ccgcagctcg ccgatggcga tcggtgcggc ggcgatcgca ctcagctgcg
     3181 tgttcctcgc cctcttcgcc aacctgatcg cgccgcacaa cccgttcgac ctggcgacgc
     3241 tggaactcag cgactcgatg ctgccgccgg cctggatgca cggcggtcag ggcaagtacc
     3301 tgctcggcac cgacgagcag ggccgcgacg tattgtcggc gctcatgttc ggcgcgcgca
     3361 tctcgctgct gatcggtggt gcctcggtga tcgtgtcggt gctgatcggc gtctcgctcg
     3421 gtctgctctc gggctacgct ggcgggcgca ccgacgcgtt catcatgcgg gtgtgcgatg
     3481 tgatgctgtc gttcccgtcg atcctgatcg ccttgctgat cgccggcgtc ggccgcgcgc
     3541 tgtttccgca ggcgcacgac tcgctcgcgt tcggcgtgct gatcatcgcg atcgcgctgc
     3601 ccggctgggt gcagtacgcg cgcacggtgc gcggctcgac gatggtcgag cgcagcaagg
     3661 agtacgtgca ggcggcgcgc gtcgtcggcg tgccgccgct gcgcatcatg ctcaggcacg
     3721 tgctgcccaa cgtgctcggg ccggtgctgg tgctggcgac gatccacctc gcgctggcga
     3781 tccagaccga agcgacgctg tcgttcctgg gcgtcggcgt gtcgccgacc tcgccgtcgc
     3841 tgggcacctt catcaacgag ggcaacaagc agttgttcag cggcgcctgg tggctggtga
     3901 tctttccagg cgccatgctg ctgctgatcg cgctgagcat gaacttgttc ggcgattggt
     3961 tgcgcgacgc gctgaacccg cgcctgcgtt gaaccggtgg gcgattcatc ctcatgacca
     4021 ccccgctgct ggaagttcgc aacctgcgcg tggaattccc gacgcgccgc ggcaagctcg
     4081 tggcgctcga cgacatctcg ttcgacatcg cgccgggcga gattctcggc gtggtcggcg
     4141 aatccggtgc gggcaagtcg ctgaccggcg cggccatcat cggcctgctc gacccgcccg
     4201 ggcacgtggc ggccggcgag atccgattcg acggccggcg catcgacaac ctgccgtacg
     4261 aggagatgcg caaggtccgc ggccgccaga tcggcgcgat cttccaggat ccgctgacct
     4321 cgctgaaccc gctgtacacc gttgggcggc agctgatcga gaccatccgc acgcacctgc
     4381 cggtgagtgc cgacgaggcg cgccggcgcg cgatcaagct gctgcaggag accggcatcc
     4441 cggcggcgca ggcgcgcatc gaccagtacc cgcaccagtt ctcgggcggc atgcgccagc
     4501 gcgtggtgat cgcgctcgcg ctcgcggccg agccgaagct gatc
//
LOCUS       DATMJB010000684         4228 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_867185, whole genome shotgun sequence.
ACCESSION   DATMJB010000684 DATMJB010000000
VERSION     DATMJB010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_867185"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..769
                     /gene="ccmC"
                     /locus_tag="VNU48_14465"
     CDS             26..769
                     /gene="ccmC"
                     /locus_tag="VNU48_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HWI12534.1"
                     /translation="MSSLYRFASPSRFDALAAVLLPWLWGTAALLAAAGLYIGFFVAP
                     TDAQQGEAYRIIFIHVPAAWMSMVIYLAMAGWAALGWVLNARMASMMARALAPSGLVF
                     TLIALWTGAAWGKPAWGTWWVWDARLTSELILLFLYLGHLALTEAIDEPRRADRAGAL
                     VAIVGAINVPVIYFSVRWWNTLHQGASVSLTAAPTMASAMLLAMLLMTFAFWAYAFAV
                     VFMRARAIVLERERDAAWVQGLTTIGRVT"
     gene            766..915
                     /gene="ccmD"
                     /locus_tag="VNU48_14470"
     CDS             766..915
                     /gene="ccmD"
                     /locus_tag="VNU48_14470"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HWI12535.1"
                     /translation="MTWHSTHEFFAMGGYGAYVWSAYAVTAALLGVEAWLARRRRRRA
                     LERRP"
     gene            912..1358
                     /gene="ccmE"
                     /locus_tag="VNU48_14475"
     CDS             912..1358
                     /gene="ccmE"
                     /locus_tag="VNU48_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HWI12536.1"
                     /translation="MTPRGRRAVLLVCVVGALGGAAALVLNAFRSNLVFFYSPSQVAA
                     KESPAGRSFRLGGLVERGSLQRDGLSVRFLVSDTVRKVPVRYEGILPDLFKEGKGVVA
                     QGQLGADGVFVAREVLAKHDENYMPPEAGAALARARAGQLLVEVSR"
     gene            1355..3370
                     /locus_tag="VNU48_14480"
     CDS             1355..3370
                     /locus_tag="VNU48_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HWI12537.1"
                     /translation="MIPELGQTALLLALAVALIQGTLPLLGAARGHEAWMRLARPAAQ
                     AQCALVTLAFALLAGSFVRNDFSLLYVAMHSNTSLPLAYRISGVWGGHEGSLLLWLTM
                     LGGWMLALARFSHQIPLPVLARTLAVMGWIAAGFLLFMLATSNPFERLVPVPPDGNDL
                     NPLLQDPGMVIHPPLLYMGYVGFSVAFAFAVAALIGSDLGTRWARWMRPWTSAAWSFL
                     TLGILLGSAWAYSVLGWGGWWFWDPVENASFMPWLAGTALIHSLAVTEKRGAFKSWTV
                     LLAIVAFSLSLLGTFLVRSGVLSSVHAFATDPKRGLFILVFLVIVIGGSLLLYAWRAP
                     KLGLGARFDAVSRESMLLLNNVLFVVAMASVLLGTLYPLLLDALGLGKISVGPPYFDA
                     VFVPLMAPVAFLMGIGPLARWKRTELPAIARRLRWAAAVSVVAALGAGWLAGRITLAA
                     SLGLWMSAWIAASIATDLWQRLGSADATPLHVRLRALPRPLVGMLLAHLGVAAFAFGV
                     SMVKTYETERDVQMAVGDSTEVAGHVFTLQGLQRVAGPNYDAVQALLEVTREARPVAL
                     LRPEKRVYRVQGTAMTEAAIDHGLTRDLYVSLGEPLAGGAWIVRVYCKPFVNWIWGGC
                     LMMALGGALAASDRRYRAKQPRAVATAAGLGVPDAVSGTVQIAGAAR"
     gene            3367..3897
                     /locus_tag="VNU48_14485"
     CDS             3367..3897
                     /locus_tag="VNU48_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HWI12538.1"
                     /translation="MTRWRFALPLLLFGLLALALARGLRLDPHELPSPLIDQPAPPFA
                     LARLDDAARTIRRDDLLGQVWVLNVWASWCEACRAEQPSLLAFARTAGVPLIGLNYKD
                     TRAQGSAWLERFGNPFDASLFDATGQVGIDFGVYGVPETFVIDKRGLIRLKLVGPLTS
                     EAIETRIRPLLKALDV"
     gene            3890..>4228
                     /locus_tag="VNU48_14490"
     CDS             3890..>4228
                     /locus_tag="VNU48_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HWI12539.1"
                     /translation="MSRVCAAWLLALCAVAAVAKDAPPVAADPALEARMLSIAAELRC
                     LVCQNQTLADSDAGLAQDLREQIRTLLRQGADAAQVRRFMTDRYGDFVLYRPPLQAST
                     LALWLGPGLLL"
BASE COUNT          477 a         1479 c         1551 g          721 t
ORIGIN      
        1 ttgcgcctgg cactggagta gcgcgatgtc gagcctgtat cgcttcgcct cgccgtcgcg
       61 cttcgatgcg ctggccgccg tgctgctgcc atggctgtgg ggcacggctg cgctgctcgc
      121 tgccgcgggg ctgtacatcg gcttcttcgt tgcgcccacc gatgcacagc agggcgaggc
      181 gtatcgcatc atcttcatcc acgtgccggc ggcgtggatg tcgatggtga tctacctcgc
      241 gatggccggc tgggcggcgc tcggctgggt gctgaacgcg cgcatggcct cgatgatggc
      301 gcgcgcactc gcgcccagcg gcctggtgtt cacgctcatc gcgctgtgga ccggcgccgc
      361 ctggggcaag ccggcctggg gcacctggtg ggtctgggac gcgcggctga cctcggagct
      421 gatcctgctg ttcctctacc tcggccacct cgcgctgacc gaggcgatcg acgagccgcg
      481 gcgcgccgac cgcgccggcg cgctggtggc aatcgtcggc gcgatcaacg tgcccgtcat
      541 ctacttctcg gtgcgctggt ggaacacctt gcaccagggc gcgagcgtca gcctcacggc
      601 cgcgccgacg atggccagcg cgatgctgct cgcgatgctg ctgatgacct tcgcgttctg
      661 ggcctatgcg ttcgcggtcg tcttcatgcg cgcacgcgcg atcgtgctcg agcgcgaacg
      721 cgacgcggcc tgggtgcagg ggctgacgac catcggacgc gtgacatgac ctggcacagc
      781 acgcacgaat tcttcgcgat gggcggctac ggcgcctacg tgtggagcgc gtatgcggtc
      841 accgcggccc tgctcggggt cgaggcctgg ctcgcgcggc ggcgccggcg ccgcgcgctg
      901 gagcggcggc catgacgccg cgcggccggc gcgccgtgct gctcgtctgt gtcgtcggtg
      961 cgctgggcgg cgccgccgcg ctggtgctca acgcgttccg cagcaacctc gtgttcttct
     1021 attcgccgtc gcaggtggcg gcgaaggaat cgcccgcggg ccgcagcttc cgactcggtg
     1081 gcctggtcga gcgcggcagc ctgcagcgcg acggcctgag cgtgcgcttc ctcgtcagcg
     1141 acacggtgcg caaggtgccg gtgcgttatg aaggcatcct gcccgacctg ttcaaggagg
     1201 gcaaaggcgt cgtcgcgcag ggccaactcg gcgccgacgg cgtgttcgtc gcgcgcgagg
     1261 tgctggccaa gcacgacgag aactacatgc cgcccgaagc cggcgccgca ctggcgcgtg
     1321 cgcgcgccgg tcaactgctc gtcgaggtca gtcggtgatc cccgagctcg gccagaccgc
     1381 gctgctgctc gcgctcgcgg tcgcgctgat ccagggcacg ctgccgctgc tcggcgcggc
     1441 gcgcggccac gaggcctgga tgcgcctggc gcggccggcc gcgcaggcgc agtgcgcgct
     1501 cgtgacgctc gcgttcgcgc tgctcgccgg cagcttcgtg cgcaacgact tctcgctgct
     1561 gtatgtggcc atgcactcga acaccagcct gccgctggcc taccgcatct ccggcgtctg
     1621 gggcggccac gaaggctcgc tgctgctgtg gctgacgatg ctgggcggct ggatgctcgc
     1681 gctcgcgcgc ttcagccacc agattccgct gccggtgctg gcgcgcacgc tcgcggtgat
     1741 gggctggatc gcggccggct tcctgctgtt catgctcgcg acctcgaacc cgttcgagcg
     1801 cctggtgccg gtgccgccgg acggcaacga cctgaacccc ttgctgcagg acccgggcat
     1861 ggtgatccac ccgccgctgc tgtacatggg ctatgtcggc ttctcggtcg cgttcgcgtt
     1921 cgcggtggcc gcactcattg gcagcgacct cggcacgcgc tgggcgcgct ggatgcggcc
     1981 gtggacgagc gccgcgtggt cgttcctgac cctgggcatc ctgctcggca gcgcctgggc
     2041 ctattcggtg ctgggctggg gcggctggtg gttctgggac ccggtcgaga acgcctcgtt
     2101 catgccctgg ctcgccggca ccgcgctgat ccactcgctg gccgtcaccg aaaagcgcgg
     2161 cgccttcaag agctggaccg tgctgctcgc gatcgtcgcg ttctcgctgt cgctgctcgg
     2221 gacctttctg gtgcgctccg gcgtgctgtc gtcggtgcac gcgttcgcga ccgatccgaa
     2281 gcgcgggctg ttcatcctcg tgttcctcgt gatcgtgatc ggcggttcgc tgctgctcta
     2341 tgcgtggcgc gcgccgaagc tcgggctggg cgcgcgcttc gatgcggtct cgcgcgagtc
     2401 gatgctgctg ctcaacaacg tgctgttcgt cgtcgcgatg gcgtcggtgc tgctcggcac
     2461 gctgtacccg ctgctgctcg atgcgctcgg gctgggcaag atctccgtcg ggccgccgta
     2521 cttcgacgcg gtgttcgtgc cgctgatggc gccggtcgcg ttcctgatgg gcatcggccc
     2581 gctggcacgc tggaagcgca ccgagctgcc ggcgatcgcg cggcggttgc gctgggccgc
     2641 ggcggtctcg gtcgtcgcgg cgctgggcgc gggatggctc gccgggcgca tcacgctcgc
     2701 ggcgagcctc ggcctgtgga tgagcgcgtg gatcgccgcg tcgatcgcga ccgacctatg
     2761 gcagcgcctc ggctcggcag atgcaacgcc gttgcatgta cgcctgcgcg ccctgccgcg
     2821 tccgctcgtg ggcatgctgc tcgcgcacct gggcgtggcg gcgttcgcgt tcggcgtcag
     2881 catggtcaag acctacgaga ccgagcgcga tgtgcagatg gcggtcggcg acagcaccga
     2941 agtcgccggc cacgtgttca cgctgcaagg gctgcagcgc gtggcggggc cgaactacga
     3001 cgcggtgcag gcgctcctcg aggtcacgcg cgaggcgcgg ccggtcgccc tgctgcgccc
     3061 cgagaagcgc gtctaccgcg tccagggcac ggcgatgacc gaggccgcga tcgaccacgg
     3121 cctgacgcgc gacctctacg tgtcgctcgg cgagccgctg gccggcgggg cgtggatcgt
     3181 gcgcgtctat tgcaagccgt tcgtgaactg gatctggggc ggctgcctga tgatggcgct
     3241 gggcggcgcg ctcgccgcga gcgaccggcg ctaccgcgcg aagcagccgc gcgcggtggc
     3301 gacggctgcc gggctcggcg tgcccgatgc ggtgagcggc accgtgcaaa tcgccggagc
     3361 cgcgcgatga cgcgctggcg tttcgcgttg ccgttgctgc tgttcgggct gctcgcgctg
     3421 gcgctcgcgc gcggcctgcg gctcgacccg cacgaactgc cgtcgccgct gatcgaccag
     3481 ccggcgccgc cgttcgcgct cgcgcggctc gacgacgccg cgcgcacgat ccgccgcgac
     3541 gacctgctcg gccaggtctg ggtgctcaac gtctgggcct cgtggtgcga ggcctgccgc
     3601 gcggagcagc catcgctgct cgcgttcgcc cgcaccgccg gcgtgccgct gatcggcctg
     3661 aactacaagg acacgcgggc gcagggcagc gcgtggctcg agcgcttcgg caatccgttc
     3721 gatgcctcgc tgttcgacgc gaccggccag gtcggcatcg acttcggcgt ctacggcgtg
     3781 cccgagacct tcgtgatcga caagcgcggg ctgatccgcc tcaagctggt cggcccgctg
     3841 acgagcgaag cgatcgagac acggattcgg ccgctgctga aggcgctcga tgtctagggt
     3901 ctgcgccgcc tggctgctgg cgctgtgcgc ggtcgcggcg gtcgcgaagg acgcgccacc
     3961 ggtcgccgcc gacccggcgc tggaggcgcg gatgctgagc atcgccgccg aactgcgctg
     4021 cctggtgtgc cagaaccaga cgctcgccga ctccgacgcg ggcctcgcgc aggacctgcg
     4081 cgagcagatc cgcacactgc tgcgccaggg cgccgacgct gcgcaggtgc gccgcttcat
     4141 gaccgaccgc tacggcgact tcgtgctcta ccggccgccg ctgcaggcga gcacgctggc
     4201 gctgtggctg gggccgggcc tgctgctc
//
LOCUS       DATMJB010000685         4709 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_447843, whole genome shotgun sequence.
ACCESSION   DATMJB010000685 DATMJB010000000
VERSION     DATMJB010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447843"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="VNU48_14495"
     CDS             complement(<1..601)
                     /locus_tag="VNU48_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI12540.1"
                     /translation="MARKIGDVILEVRNISLAFGGVKALTDISFDVREHEIRAIIGPN
                     GAGKSSMLNCINGVYQPQQGSITFRGQTFKHMNSRQVAEMGLARTFQNLALFKGMSVL
                     DNIMSGRNLKMKSNIFQQAIRWGAAEREELAHREFVERIIDFLEIQAIRKTSAGRLPY
                     GLQKRVELARALAAEPQLLLLDEPMAGMNVEEKEDMCRFI"
     gene            complement(602..2548)
                     /locus_tag="VNU48_14500"
     CDS             complement(602..2548)
                     /locus_tag="VNU48_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWI12541.1"
                     /translation="MPTTFTRLMLDHAAQRPDAAALREKDYGIWQTLSWRALATLVRE
                     LACGLADAGLRRGQHLVVIGENRPRLYAAMLAAQSLGAVPVPLYQDAAATEYVFPITN
                     AEVAFAIVEDQEQVDKLLEVREQCAQLRSLWFDDPRGLRHYAEPGLAPLDALIAAGRA
                     YDAAHPGFFDAEIQKTQPDDVAAMFFTSGTTGNPKGVVHTHHTLIDRAQAGAKFDKLT
                     SAEEVLAYLPPAWIGQNIFSYAQWLACGYVVNCPESASTVSIDLNDIGPTYYFAPPRV
                     FEGLLTTVMIRMEDAGAIKRWLFHHFMAVARRVGPRLMDGQPVGALDRLHYAIGRLVI
                     YGPLRNTLGLSRVRVAYTAGEAIGPDLFSFYRSIGINLKQLYGSTETAVFVCLQPDHE
                     ARADTVGVPIEGVQVRVSDSGEILVKSPGLLKEYYRNPAATAEVLTADGWYHTGDAGF
                     LDAAGHLKIIDRAKDVGRLIGGAFDAQMFAPKYVENKLKFFPFIKEAVAIGDQRATVC
                     AFVNIDFEAVGNWAERRHLPYAGYADLAQKPEVYALIRECVEKVNADLAVDDKLAGSQ
                     ISRFLILHKELDADDGELTRTRKVRRGFIAERYQVLVDALYGGKTHQYVETAVKFEDG
                     RAGMVAADLKIVDTQVFPAMKRAA"
     gene            2677..3408
                     /locus_tag="VNU48_14505"
     CDS             2677..3408
                     /locus_tag="VNU48_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWI12542.1"
                     /translation="MNPVIGPPAAPTTLSLYQRARAPNASELSDIPWLPALDRDERAA
                     AVADLKVVQVAAGELLCRVGRPATYWFGVIDGLLKMSNDTAFGLPITFTGVPPGGWFG
                     EGTMIKREVYRYNIQALRKSVVAGLSVQTFHWLLDRSIPFNRFITNQLNERLGQFIAA
                     REIDRMSDADARVARSLAALFHPVLYPGVGTLLRITQQELGYLVGLSRQRVNEALKAL
                     QCQGCIRIEYGGVRVTDIDALRRIA"
     gene            complement(3479..4399)
                     /locus_tag="VNU48_14510"
     CDS             complement(3479..4399)
                     /locus_tag="VNU48_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HWI12543.1"
                     /translation="MSTSLDGQLVVAISSRALFDFEEENQHFEANDDRAYMRLQLQRL
                     DRAAKPGVAFSLVNKLLAFNAPADVSSGAPPRQRVEVVILSRNDPVSGMRVFRSAQHY
                     GLPIQRGVFTRGESPWRYLRPLNAHLFLSTNEADVRSALGAGVPAARVYPHSARASGQ
                     HPNEVRIAFDGDAVLFSDEAERVFQRGGLDAFQAHEREHSTTPLAAGPFKPLLLALRE
                     LQQAPGQAMRIRTALVTARSAPAHERAIRTLMDWNIEVDEAMFLGGLPKGEFLREFEP
                     DFFFDDQTGHVDSAAAHVPSGHVASGIRNG"
     gene            complement(4442..>4709)
                     /locus_tag="VNU48_14515"
     CDS             complement(4442..>4709)
                     /locus_tag="VNU48_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWI12544.1"
                     /translation="RSPDRDAARHIAAKLYGHIRAEHGWGERFDTEPASDVLDAMAEM
                     APREMRRAWMTAFGNAKLDGRDRVEPADLPETRGKRGAIGFATH"
BASE COUNT          701 a         1586 c         1634 g          788 t
ORIGIN      
        1 ggatgaagcg gcacatgtct tccttctcct cgacgttcat ccccgccatc ggctcgtcga
       61 gcagcaggag ctggggctcg gcggccaggg cccgggccag ttccacccgc ttctgcaggc
      121 cgtacggcaa ccggccggcc gacgtcttgc ggatggcctg gatctccagg aagtcgatga
      181 tccgctcgac gaactcgcgg tgcgccagtt cctcgcgttc ggccgcgccc cagcggatcg
      241 cctgctggaa gatgttcgat ttcatcttca ggttgcgccc gctcatgatg ttgtcgagca
      301 cgctcatgcc cttgaacagg gccaggttct ggaacgtgcg cgccaggccc atctcggcga
      361 cctggcgcga attcatgtgc ttgaaggtct ggccgcgaaa cgtgatcgag ccctgctgcg
      421 gctggtagac gccgttgatg cagttcagca tcgagctctt gccggcgccg ttcgggccga
      481 tgatcgcgcg gatctcgtgc tcgcgaacgt cgaagctgat gtcggtcagc gccttcacgc
      541 cgccgaacgc gaggctgatg ttcctgacct cgaggatcac gtcgccgatc ttgcgggcca
      601 tctaggcggc cctcttcatc gcggggaaca cctgggtgtc gacgatcttc agatcggccg
      661 cgaccatgcc tgcgcggcca tcctcgaact tgaccgcggt ctcgacgtat tgatgggtct
      721 tgccgccgta caacgcatcg acaagcacct ggtagcgctc ggcgatgaaa ccgcgccgca
      781 ccttgcgcgt gcgcgtcagt tcgccgtcgt cggcgtcgag ttccttgtgc aggatcagga
      841 agcggctgat ctgcgagccg gcgagcttgt cgtccacggc gaggtcggcg ttgacctttt
      901 cgacgcactc gcggatcagt gcgtagacct cgggcttctg cgccagatcg gcgtagccgg
      961 cgtagggcag gtggcgccgt tcggcccagt tgccgacggc ctcgaagtcg atgttgacga
     1021 aggcgcagac cgtggcgcgc tggtcaccga tcgcgaccgc ctccttgatg aacgggaaga
     1081 acttcagctt gttctcgacg tacttcggcg cgaacatctg cgcatcgaac gcgccgccga
     1141 tgaggcggcc gacgtccttg gcacggtcga tgatcttcag gtggccggcg gcgtcgagga
     1201 agccggcgtc gccggtgtgg taccagccgt cggccgtcag cacctcggcg gtggccgccg
     1261 ggttgcggta gtactccttc agcagtcccg gcgacttgac caggatctcg ccgctgtcgc
     1321 tgacccggac ctgcacgccc tcgatcggca cgccgacggt gtcggccctg gcctcgtggt
     1381 cgggctgcag gcagacgaag accgcggttt cggtggagcc gtagagctgc ttcaggttga
     1441 tgccgatgga gcgatagaag ctgaacaggt ccgggccgat cgcctcgccg gcggtgtagg
     1501 ccacgcgcac gcggctcaag cccagggtgt tgcgcagcgg gccgtagatc acgagccgac
     1561 cgatcgcgta gtgcagacgg tcgagcgcgc ccaccggctg cccgtccatc agccgcggcc
     1621 cgacgcgccg cgccaccgcc atgaagtgat ggaacagcca ccgcttgatc gcgccggcgt
     1681 cctccatgcg gatcatcacg gtggtcagca ggccctcgaa gacgcgcggc ggcgcgaagt
     1741 agtaggtcgg cccgatgtcg ttcaaatcga tgctgaccgt gctcgccgat tccgggcagt
     1801 tgacgacgta gccgcacgcc agccactgcg cgtacgagaa gatgttctgg ccgatccagg
     1861 ccggcggcag gtaggccagc acttcctcgg cgctcgtgag cttgtcgaac ttcgcacccg
     1921 cctgtgcgcg gtcaatcagc gtgtggtggg tgtggacgac gcccttcggg ttgccggtgg
     1981 tgcccgacgt gaagaacatc gcggcgacgt cgtcgggctg cgtcttctgg atctcggcat
     2041 cgaagaagcc ggggtgcgcg gcgtcgtagg cccggcctgc agcgatcagt gcgtcgagcg
     2101 gggcgaggcc cggttcggcg taatgacgca ggccgcgcgg gtcgtcgaac cacaggctgc
     2161 gcagctgcgc gcactgctcg cgcacttcga gcagcttgtc gacctgctcc tggtcttcga
     2221 cgatcgcgaa cgcgacctcg gcattggtga tcgggaacac gtactcggtc gccgcggcgt
     2281 cctggtacag cggcaccggc accgcgccca gcgactgggc ggccagcatc gcggcgtaca
     2341 ggcgcggccg gttctcgccg atcaccacga ggtgctggcc acgccgcagg ccggcgtcgg
     2401 ccaggccgca cgccagctcg cgcaccagcg tggcgagcgc gcgccagctg agcgtctgcc
     2461 agatgccgta gtccttctca cgcaacgcgg cggcgtcggg ccgctgcgcc gcgtggtcaa
     2521 gcatcaggcg tgtgaacgtg gtgggcaccg caggtctccg gtcgtggcgt cgggtcacac
     2581 gggtgacacc gccagagcga atgtagagac aggtttgacg ccatgttgtc gttctgacga
     2641 caatcctagg gtcgcccctc tccggtgttt cccttgatga atccagtcat cgggccgcct
     2701 gccgcgccga ccacgctgtc gctgtaccag cgcgcgcggg cgccgaatgc ctcggagctc
     2761 tccgacatcc cgtggctgcc ggcgctggac cgcgacgagc gcgccgcagc cgtggccgat
     2821 ctgaaggtcg tgcaggtcgc ggccggcgaa ttgctctgcc gggtcggccg gcccgcgacc
     2881 tactggttcg gcgtgatcga cggccttctg aagatgagca acgacaccgc gttcggcctc
     2941 ccgatcacct tcaccggcgt gcccccgggg ggatggttcg gcgagggcac catgatcaag
     3001 cgcgaggtct atcgctacaa catccaggcc ttgcgcaaga gcgtggtcgc gggcctgagc
     3061 gtgcagacct ttcactggct gctcgaccgg agcatcccgt tcaaccgctt catcaccaac
     3121 cagctcaacg agcggctcgg gcagttcatc gcggcgcgcg agatcgaccg catgagcgac
     3181 gccgacgcgc gcgtcgcgcg cagcctcgcg gcgctgttcc atccggtgct gtacccgggc
     3241 gtgggcacgc tgttgcgcat cactcaacag gagctggggt acctggtggg cctgtcgcgt
     3301 cagcgcgtca acgaggcatt gaaggcattg caatgccagg gctgcatccg catcgagtac
     3361 ggcggcgtgc gcgtcaccga catcgacgcg ctgcgccgga tcgcgtaggc gtgcgcggcg
     3421 cggtgcgcca tccccggatc ctcagttgag gggccgcgca acgggccggc gcgccccgtc
     3481 agccattgcg aatacccgac gcgacgtggc cggacggcac gtgcgccgcg gccgaatcga
     3541 cgtgcccggt ctggtcgtcg aaaaagaaat cgggttcgaa ttcgcgcagg aactcgccct
     3601 tcggcagccc gcccagaaac atcgcctcgt cgacctcgat gttccagtcc atcagcgtgc
     3661 ggatcgcgcg ttcgtgcgcc ggtgcgctgc gcgccgtcac gagcgcggtg cggatgcgca
     3721 tcgcctgccc cggcgcctgc tgcaactcgc gcagcgccag cagcagcggc ttgaacgggc
     3781 cggccgccag cggcgtggtc gagtgttcgc gctcgtgggc ctggaacgcg tcgagcccac
     3841 cgcgctggaa cacgcgctcg gcttcgtccg agaacagcac cgcgtcgccg tcgaaggcga
     3901 tgcgcacctc gttcgggtgc tggcccgatg cgcgcgccga gtgcgggtag acgcgcgccg
     3961 ccggcacgcc ggcgccgagt gccgagcgca catcggcttc attggtcgac aggaacaggt
     4021 gcgcgttcag cgggcgcagg tagcgccacg gcgattcacc gcgcgtgaac acgccgcgct
     4081 ggatcggcag cccgtagtgt tgcgccgagc ggaacacgcg catgccggag accgggtcgt
     4141 tgcgcgacag gatcaccact tcgacacgct ggcgcggcgg cgcgccggaa ctcacatccg
     4201 ccggggcatt gaacgcgagc agcttgttga ccagcgagaa cgcgacgccg ggcttggccg
     4261 cgcggtccag gcgctgcaac tgcagccgca tgtaggcgcg gtcgtcgttc gcctcgaagt
     4321 gctggttctc ttcctcgaag tcgaagaggg cgcgcgacga gatcgcgacg acgagctggc
     4381 cgtcgagtga agtggacatg cgctcgatga tacgagcgcc gtcctggcga gccgggtgcc
     4441 gtcagtgggt cgcgaacccg atcgcgccgc gcttgccgcg cgtctcgggc aggtcggccg
     4501 gttcgacgcg gtcgcggccg tcgagcttgg cgttgccgaa cgcggtcatc caggccctgc
     4561 gcatctcgcg cggcgccatc tcggccatcg cgtcgagcac gtcgctcgcc ggttcggtgt
     4621 cgaagcgctc gccccagcca tgctcggcgc ggatgtggcc atacagcttc gccgcgatgt
     4681 ggcgtgcggc gtcgcggtcc ggcgagcgc
//
LOCUS       DATMJB010000686         2284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_329656, whole genome shotgun sequence.
ACCESSION   DATMJB010000686 DATMJB010000000
VERSION     DATMJB010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329656"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..624)
                     /locus_tag="VNU48_14520"
     CDS             complement(241..624)
                     /locus_tag="VNU48_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HWI12545.1"
                     /translation="MIRVVRLGSPRVDAEGPRIGTVRRPPRGVPKAEFARQDWYDVWF
                     PNLAPSADTVKLGQDAKTPAEWAAFLRKYRSEMTAPEASHALELLATLSHHSNFSVGC
                     YCEDEAHCHRSALRALLIDKGAKVE"
     gene            complement(621..1124)
                     /locus_tag="VNU48_14525"
     CDS             complement(621..1124)
                     /locus_tag="VNU48_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HWI12546.1"
                     /translation="MSGDATVFDGGCTCRALRYRMVDRPLFVHCCHCRWCQRETGASF
                     ALNAMIESDRVELLQGDVVLVDTPSNSGLGQKIARCPACHVAVWSNYGGGGDAVRFVR
                     VGTLDEPDRLPPDIHIFTASKQPWVVLPPGTPAVAEYYKASELWPKQSLERRAALQGR
                     SQPGRSS"
     gene            complement(1169..1588)
                     /locus_tag="VNU48_14530"
     CDS             complement(1169..1588)
                     /locus_tag="VNU48_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWI12547.1"
                     /translation="MITSSTEKPASATLRGSILYVEDEPISFAVVEALLASHPDVRLI
                     RAQTGDEGVRLARSEHPDALLLDMHLPDISGVEVVRRLSEDISAGSFRVVLLTADKLN
                     IDVLKAMSLGAFEYLVKPVSRRALEAALERALSRKKP"
     gene            1738..2265
                     /locus_tag="VNU48_14535"
     CDS             1738..2265
                     /locus_tag="VNU48_14535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HWI12548.1"
                     /translation="MPVRATRGRRDNAPTMPRLQTVPSPVTDPVDAPPRSKGRRGDPQ
                     KFAVDVKASRIDGQGAFAAEAIPARRKIGEIRGESVSVREARRRARGQQRIMIVELSD
                     RRAIDASNSGDPLRFTNHSCRPNAVLRICQGRVEFYAMRDVAPGEEITVNYGETHHEG
                     RLRCRCGAAGCIGRL"
BASE COUNT          381 a          758 c          797 g          348 t
ORIGIN      
        1 gccggtcatc ggcctgcacg gtggagcaga tcgggccggt ggggcgtgct gcgcagcgaa
       61 gtaaaggagg aggggttggc cgcaggccga ccccggggga catgagcgca gcagcatgcc
      121 ccgccggccc gatcccgcgc cgatacgtgc acggcggaca ttcaccgaca gcatcgggcc
      181 gaaagccgcc acgccgtcac aactccatgc ggccagtttc atttcaggct tgaagaatcc
      241 tcattcgacc ttcgcgccct tgtcgatcag cagcgcgcgc agcgccgagc gatggcagtg
      301 cgcttcatcc tcgcagtagc agcccaccga gaagttgctg tggtgcgaga gcgtggccag
      361 cagttcgagg gcatggctgg cctcgggcgc ggtcatttcg ctgcggtact tgcgcaggaa
      421 cgcggcccac tcggccgggg tcttcgcgtc ctgcccgagc ttgacggtgt cggcgctcgg
      481 cgcgaggttc gggaaccaga cgtcgtacca gtcctggcgt gcgaactccg ccttcggcac
      541 accgcgcggc gggcgccgca cggtgccgat gcgcgggcct tcggcatcga cgcgcgggct
      601 gcccagcctg acgacgcgaa tcacgacgac cgaccaggct gcgagcggcc ctgcaacgcg
      661 gcgcggcgct cgaggctttg cttcggccag agctcggagg ccttgtagta ctcggccacg
      721 gcgggcgtgc ccggcggcag cacgacccag ggctgcttgg acgcggtgaa gatgtggatg
      781 tcgggcggca ggcgatcggg ctcgtcgagc gtgccgacgc gaacgaaccg gaccgcatcg
      841 ccgccgccgc cgtagttgct ccagacggcg acatggcagg ccgggcagcg cgcgatcttc
      901 tgccccaggc cgctgttcga cggcgtgtcg acgagcacca cgtcgccctg cagcagctcg
      961 acgcggtcgg actcgatcat cgcgttcagc gcgaacgacg cgccggtctc gcgctggcac
     1021 cagcggcagt ggcagcagtg cacgaacagg ggtcggtcga ccatgcggta gcgcagcgcg
     1081 cggcaggtgc agccgccgtc gaagacagtg gcgtcgccgc tcatcgcacc gctcgcggtc
     1141 tcacgctcga catttcaacc ggcgcgctct acggcttctt gcgcgagagc gcacgttcca
     1201 aggcggcttc gagcgcccgg cggctcaccg gtttgaccag atactcgaac gcaccgaggc
     1261 tcatggcctt gagcacgtcg atgttgagct tgtccgcagt caaaagaacg acgcgaaacg
     1321 aaccggcaga gatgtcttca ctgagcctgc gaacgacttc aacgccggaa atgtccggga
     1381 ggtgcatatc cagcagcagg gcgtcggggt gttcgcttcg ggcgagtctc acgccttcgt
     1441 ctccggtctg cgctcgaatg aggcgcacgt ccggatggct cgccaagagg gcttccacca
     1501 cggcaaagct gatgggttca tcctcgacat acaggattga gcctcgcagc gttgccgagg
     1561 caggcttctc agtcgaggag gtaatcatgg cgcgctccac ggaggcagtg agccgtaagg
     1621 atagatcctt gcaaggagag cgcttggaag tggggtgatt gcagaatgtc gggcgccgat
     1681 cagtgcgtgt tcccttgaac ggcgcgttca tctccgatcg ctgggtgaac tgaccggatg
     1741 cccgtgcgcg ccacccgcgg ccgacgcgac aatgcgccga ccatgccgcg ccttcagacc
     1801 gttccctcgc ccgtcaccga ccccgtcgac gcgccgccgc ggagcaaagg ccgacgcggc
     1861 gatccgcaga agttcgccgt cgatgtgaag gccagccgca tcgacgggca aggtgccttc
     1921 gccgccgagg cgattccggc gcggcgcaag atcggagaga tccgtggcga gtcggtcagc
     1981 gttcgcgagg cgcggcggcg cgcgcgcgga cagcagcgca tcatgatcgt cgagctgagc
     2041 gatcgccgcg cgatcgacgc ctccaactcg ggcgacccgc tgcgtttcac caaccactcc
     2101 tgccggccga atgcggtgct gcgcatctgc caggggcgcg tcgagttcta cgcgatgcgc
     2161 gacgtggcac cgggcgagga gatcaccgtc aactacggtg aaacgcacca cgagggccgg
     2221 ctgcgctgcc gttgcggcgc ggccggctgc atcggccggc tgtgagcgaa gacggccgag
     2281 cgct
//
LOCUS       DATMJB010000687         2703 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_548353, whole genome shotgun sequence.
ACCESSION   DATMJB010000687 DATMJB010000000
VERSION     DATMJB010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548353"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VNU48_14540"
     CDS             complement(<1..850)
                     /locus_tag="VNU48_14540"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWI12549.1"
                     /translation="MGLLSWAIWLPILSGVALLAVVRDPGTARWLALATALASFAVTL
                     PLIGGFDNATAAMQFVERFDWIERFNVHYHLGIDGLSLWFVPLTAFITVIVVISAWEV
                     ITDRVNQYMGAFLILSGLMVGVFAALDGLLFYVFFEATLIPMYLIIGVWGGPRRVYAA
                     FKFFLYTLAGSLLMLVALIFLYYKSGGSFDILTWHKLPLPMNAQSLLFFAFLLAFAVK
                     VPMWPVHTWLPDAHVEAPTGGSVVLAAILLKLGAYGFVRFSLPILPDASRELAWMMIG
                     LSLVAIF"
     gene            complement(870..>2703)
                     /gene="nuoL"
                     /locus_tag="VNU48_14545"
     CDS             complement(870..>2703)
                     /gene="nuoL"
                     /locus_tag="VNU48_14545"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWI12550.1"
                     /translation="NATVYEWLTLGSLKMEIGFLIDGLTAMMMCVVTFVSLMVHIYTI
                     GYMEDDPGYQRFFSYISLFTFSMLMLVMSNNFLQLFFGWEAVGLVSYLLIGFWYTRPT
                     AIFANMKAFLVNRVGDFGFILGIGLIAAYAGTLNYGEAFAKADELAKLSFPGTEWMLI
                     TVTCICLFIGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGIFMVARMSPLF
                     ELSDTALSFVMTIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTVALGASAYSVA
                     VFHLMTHAFFKALLFLGAGSVIIGMHHDQDIRNMGGLRKYMPITWITSLIGSLALIGT
                     PLFAGFYSKDSIIEAVHESHLWGSGFAYGAVMIGVFVTAFYSFRMYFLVFHGEERFRH
                     KPFPGEHDHADAHGHVHVHEPHESPWVVWVPLVALAIPSVVIGYLTIAPMLFGDFFTG
                     AIVIDAEHHEAMKELAEAFHGPAAMAVHALSSPVLWLALAGVVSAYVFYMVNPAIPAA
                     IKARFGFIYELLDNKYYFDWFNENVLARLARLTGTGLWKGGDEGLIDGVLLGGSVRTV
                     GGIAAIGRRLQSGYLYFYALVMVVGVIGLMTWQLWPFLTGSAPK"
BASE COUNT          567 a          885 c          866 g          385 t
ORIGIN      
        1 agaagatcgc caccagcgat aggccgatca tcatccacgc cagctcgcgg gacgcgtcgg
       61 gcaggatcgg gagagagaac cgcacgaagc cgtaggcgcc gagcttcagc aggatcgccg
      121 ccagcaccac cgagccgccg gtgggcgcct cgacgtgggc atccggcagc caggtgtgca
      181 ccggccacat cggcaccttg accgcgaacg cgagcaggaa cgcgaagaac agcaggctct
      241 gcgcattcat cggcagcggc agcttgtgcc aggtgaggat gtcgaagctg ccgcccgact
      301 tgtagtacag aaagatcagc gcgaccagca tcagcagcga gccggccagc gtgtacagga
      361 agaacttgaa cgccgcgtag acgcgccgcg gcccgcccca gacgccgatg atcaggtaca
      421 tcgggatcag cgtggcctcg aagaagacgt agaacagcag gccgtcgagc gccgcgaaca
      481 cgccgaccat caggcccgac aggatcagga acgcgcccat gtactggttc acgcggtcgg
      541 tgatcacctc ccaggccgag atcacgacga tcaccgtgat gaaggcggtc agcggcacga
      601 accacagcga cagaccgtcg atgccgaggt ggtagtgcac gttgaagcgt tcgatccagt
      661 cgaagcgttc gacgaactgc atcgccgcgg tggcgttgtc gaagccgccg atcagcggca
      721 gcgtgacggc gaagctcgcc agcgccgtcg cgagcgccag ccagcgcgcc gtgcccggat
      781 cgcgcacgac cgcgagcagc gccacgcccg acaggatcgg cagccagatc gcccaactca
      841 acaaacccat gttcttgttc tccgtgcggc tacttcggcg ccgagccggt caggaacggc
      901 cacagctgcc aggtcatcag cccgatcacg ccgacgacca tcaccagcgc atagaagtac
      961 aggtagccgc tttgcagccg gcgcccgatc gccgcgatgc cgccgacggt gcgcaccgaa
     1021 ccgccgagca gcacgccgtc gatcaggccc tcgtcgccgc ccttccacag gccggtgccc
     1081 gtcagccgcg ccaggcgcgc cagcacgttc tcgttgaacc agtcgaagta gtacttgttg
     1141 tcgagcagct cgtagatgaa gccgaacctt gccttgatcg cggcggggat cgccggattg
     1201 accatgtaga agacgtacgc cgagacgacc cccgccagcg cgagccagag caccggcgag
     1261 ctcagcgcat gcacggccat cgcggccggg ccgtggaagg cctcggcgag ttccttcatc
     1321 gcctcgtggt gctcggcgtc gatgacgatc gcgcccgtga agaaatcgcc gaacagcatc
     1381 ggcgcgatcg tcaggtagcc gatgaccacc gacgggatcg ccagcgccac cagcggcacc
     1441 cagacaaccc agggcgactc gtgcggctcg tggacatgca cgtggccgtg cgcgtcggcg
     1501 tggtcgtgct cgcccgggaa cggcttgtgg cggaagcgct cctcgccgtg gaagaccagg
     1561 aagtacatcc ggaacgaata gaacgccgtg acgaacacgc cgatcatcac cgcgccgtac
     1621 gcgaagcccg atccccacag atggctctcg tgcaccgcct cgatgatgct gtccttcgag
     1681 tagaagcccg cgaacagcgg cgtgccgatc agcgccagcg agccgatcag cgaggtgatc
     1741 caggtgatcg gcatgtactt gcgcaacccg cccatgttgc gaatgtcctg gtcgtggtgc
     1801 atgccgatga tcaccgagcc ggcaccgagg aacagcagcg ccttgaagaa cgcgtgcgtc
     1861 atcaggtgga acaccgcgac cgaataggcc gaggcgccga gcgcgaccgt catgtagccg
     1921 agctggctca gggtcgagta cgcgacgacg cgcttgatgt cgttctggat cacgcccaga
     1981 aaacccatga acagcgccgt gatcgcaccg atcgtcatca cgaacgacag cgccgtgtcc
     2041 gacagctcga acagcggcga catgcgcgcg accatgaaga tgcccgctgt caccatcgtc
     2101 gcggcgtgga tcagcgccga gatcggcgtc gggccttcca tcgaatcggg cagccacacg
     2161 tgcagcggga actgcgcgct cttgcccatc gcgccgatga acaggcagat gcaggtcacg
     2221 gtgatcagca tccactcggt gccggggaag ctcagcttgg ccagttcatc ggccttggcg
     2281 aaggcctcgc cgtagttcag cgtgccggca taggcggcga tcaggccgat gccgaggatg
     2341 aagccgaagt cgcccacgcg gttgaccagg aacgccttca tgttggcgaa gatggccgtc
     2401 ggacgcgtgt accagaagcc gatcagcagg tacgagacaa ggcccaccgc ttcccagccg
     2461 aagaacagct gcaggaagtt gttgctcatc accagcatca gcatcgagaa ggtgaacagc
     2521 gagatgtagc tgaagaagcg ctggtagccc gggtcgtcct ccatgtagcc gatggtgtag
     2581 atgtggacca tcagcgacac gaaggtcacg acgcacatca tcatcgcggt caggccgtcg
     2641 atcaggaagc cgatttccat cttcaggctg ccgagcgtca gccattcgta gacggtggcg
     2701 ttg
//
LOCUS       DATMJB010000688         2331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_33934, whole genome shotgun sequence.
ACCESSION   DATMJB010000688 DATMJB010000000
VERSION     DATMJB010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33934"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VNU48_14550"
     CDS             <1..781
                     /locus_tag="VNU48_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWI12551.1"
                     /translation="DWTTAIAFRYRRRGGSGLIEPVRHVAPIRLAALVEVEPQKERLL
                     RNTRQFVDGLMANNVLLTGARGTGKSSLIKACLNEFADRGLRLIEVDKTDLVDLPDIV
                     DLVAERPERFIVFCDDLSFDEGEPGYKALKSILDGSVAQASDNVLIYATSNRRHLLPE
                     YMKENLSYQHLDNGEVHPGEVVEEKISLSERFGLWVSFYPFSQDEYLAIVAQWLRSFS
                     VPEDAIVAARQESLVWALERGSRSGRVAFQFAKDYSGRHHA"
     gene            778..1215
                     /locus_tag="VNU48_14555"
     CDS             778..1215
                     /locus_tag="VNU48_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWI12552.1"
                     /translation="MSGIEARLPIDVAVGVLINPQGEFLLTSRPAGKVYAGYWEFPGG
                     KLEAGESVEAALRRELIEEIGIEIGAVQPWKIELVDYEHARVRLHFCKVFEWSGAFEM
                     RERQAMAWQQLPVRVGPVLPGTLPVLRWFAEERGFPGATTLAG"
     gene            1212..1601
                     /gene="hisI"
                     /locus_tag="VNU48_14560"
     CDS             1212..1601
                     /gene="hisI"
                     /locus_tag="VNU48_14560"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HWI12553.1"
                     /translation="MNWLDDVKWDANGLVPAIAQELGSNDVLMFAFMNREALARTAEL
                     GEAVYFSRSRGRLWHKGEESGHIQKVHELRLDCDNDVVLLKVEQLGHAPGIACHTGRH
                     SCFFQLLKDGVWTAVEPVLKDPERIYK"
     gene            1598..1951
                     /locus_tag="VNU48_14565"
     CDS             1598..1951
                     /locus_tag="VNU48_14565"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HWI12554.1"
                     /translation="MSLNSNDTLARLAAVIEARRAADPDKSYVARLFRKGHDAILKKI
                     GEEATEVVMACKDGAPERIVGEVADLWFHCMLALSAYGLTPAEVLAELERREGLSGLE
                     EFAARKSRQRDKEGT"
     gene            1948..>2331
                     /locus_tag="VNU48_14570"
     CDS             1948..>2331
                     /locus_tag="VNU48_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12555.1"
                     /translation="MNDIIESTPNAAEREDSLRTIGHISYALHAVVAIGAVLPGGHAS
                     IVLLLVAFILDLVKKDDARGSWQESHFAWRIRSVLWAGVLYVVTIPLWFLFLIPGWIA
                     WIAISVWFLYRVIRGWLALNDRRPMQ"
BASE COUNT          381 a          744 c          839 g          367 t
ORIGIN      
        1 cgactggacc acggcgatcg cgttccgcta ccgcaggcgt ggcggctcgg ggctgatcga
       61 gccggtgcgg catgtcgcac cgatccggct ggcggcgctg gtcgaggtcg agccgcagaa
      121 ggagcggctg ctgcgcaaca cgcgccagtt cgtcgacggg ctgatggcca acaacgtgct
      181 gctgaccggc gcgcgcggca ccggcaagag ctcgctgatc aaggcctgcc tgaacgagtt
      241 cgccgaccgc gggttgcgcc tgatcgaggt cgacaagacc gacctggtcg atctgcccga
      301 catcgtcgac ctggtggccg aacggcccga gcgcttcatc gtgttctgcg acgacctcag
      361 cttcgacgag ggcgagcccg gctacaaggc gctgaagtcg atcctcgacg gctcggtggc
      421 gcaggcctcg gacaacgtgc tgatctatgc gaccagcaac cgccgccacc tgctgcccga
      481 gtacatgaag gagaacctga gctaccagca cctggacaac ggcgaggtgc accccggcga
      541 ggtcgtcgag gaaaagatct cgctgtccga gcgcttcggc ctgtgggtca gcttctaccc
      601 gttcagccag gacgagtacc tcgcgatcgt cgcgcagtgg ctgcggtctt tcagcgtgcc
      661 ggaggacgcg atcgtcgcgg cgcgccagga gagcctggtc tgggcgctgg agcgcggctc
      721 gcgctcgggg cgggtcgcgt tccagttcgc gaaggactac agcggaaggc atcacgcgtg
      781 agcggcatcg aggcgcgctt gccgattgat gtggccgtgg gcgtgttgat caacccgcag
      841 ggcgagttcc tgctgacctc gcgccccgcg ggcaaggtct acgccggcta ctgggagttc
      901 ccgggcggca agctggaggc cggcgaatcg gtcgaggcgg cgctgcggcg cgagctgatc
      961 gaagagatcg gcatcgagat cggggcggtg cagccctgga agatcgagct ggtcgactac
     1021 gagcacgcgc gcgtgcggct gcacttctgc aaggtcttcg aatggtcggg cgcgttcgag
     1081 atgcgcgagc gccaggcgat ggcctggcag cagctgccgg tgcgcgtcgg cccggtgctg
     1141 cccggcaccc tgccggtgct gcgctggttc gccgaggaac gcggctttcc cggcgccact
     1201 acactggccg gatgaactgg ctcgacgacg tgaaatggga cgcgaacggc ctcgtgccgg
     1261 cgatcgcgca ggagctcggc agcaacgacg tgctgatgtt cgcgttcatg aaccgcgagg
     1321 cgctcgcgcg caccgccgag ctcggcgagg cggtgtactt cagccgctcg cgcgggcggc
     1381 tctggcacaa gggcgaggaa agcggccaca tccagaaggt gcacgagctg cgcctcgatt
     1441 gcgacaacga cgtggtgctg ctcaaggtcg agcagctcgg ccacgcgccc ggcatcgcct
     1501 gtcacaccgg ccggcactcg tgcttcttcc agttgttgaa agatggcgtc tggaccgcgg
     1561 tcgaaccggt cttgaaagac ccggagcgca tctacaaatg agcctgaact cgaacgacac
     1621 gctggcgcgc ctggccgcgg tgatcgaggc gcgccgcgcg gccgatccgg acaagagcta
     1681 cgtcgcgcgc ctgttccgca agggccacga cgcgatcctg aagaagatcg gcgaagaggc
     1741 caccgaggtg gtcatggcgt gcaaggacgg cgcgcccgaa cgcatcgtcg gcgaggtcgc
     1801 cgacctgtgg tttcactgca tgctggcgct cagcgcctac gggctgacgc cggccgaggt
     1861 gctggccgag ctggagcgtc gcgaagggct gtcggggctg gaggaattcg cggcacgaaa
     1921 atcgcgacag cgcgacaagg agggcacatg aacgacatca tcgagagcac ccccaacgcc
     1981 gccgagcgcg aggactcgct gcgcacgatc ggtcacatca gctatgcgct gcacgcggtc
     2041 gtcgcgatcg gcgcggtatt gcccggcggg catgccagca tcgtgctgct gctggtcgcg
     2101 ttcatcctcg acctggtcaa gaaggacgac gcgcgcggca gctggcagga gtcgcacttc
     2161 gcctggcgca tccgcagcgt gctgtgggcc ggggtgctgt atgtcgtgac gatcccgctg
     2221 tggttcctgt tcctgatccc gggctggatc gcctggatcg cgatctcggt ctggttcctg
     2281 taccgcgtga tccgcggctg gctggcgctg aacgaccgcc ggccgatgca g
//
LOCUS       DATMJB010000689         3073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_93793, whole genome shotgun sequence.
ACCESSION   DATMJB010000689 DATMJB010000000
VERSION     DATMJB010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93793"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VNU48_14575"
     CDS             complement(<1..548)
                     /locus_tag="VNU48_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWI12556.1"
                     /translation="MNPAIDDLTLHAWLDGELPTQRHAEVEAWLQANPEDAARVRLWA
                     ADRELLRARLAGVLDEPVPAALAQLLSRKAPGVGAGWQRWAAALAVFALGGALGAGLM
                     WRAQAPAAALAAAEPAWVRRAAVAHAVYVPEVRHPVEVAAKEEQHLSGWLTKRLDVPV
                     KVFDLRAQGFELVGGRLLPDAAG"
     gene            complement(568..1098)
                     /locus_tag="VNU48_14580"
     CDS             complement(568..1098)
                     /locus_tag="VNU48_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWI12557.1"
                     /translation="MDAPAAFRQQLLGAIPRLRRYARSLVFDASAADDLVQTALERAL
                     AHWHQFDQRRDILVWTLSIAHNAYMDERRRHARLPLADGGDRFGALDERHAAHSPDIG
                     LQLDLVAALKRLPVEQREPLLLVTLEQLSYAECAELLRIPIGTVMSRISRARVALRAL
                     LDGRAPAAAPALRRVV"
     gene            complement(1126..1710)
                     /locus_tag="VNU48_14585"
     CDS             complement(1126..1710)
                     /locus_tag="VNU48_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020068600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWI12558.1"
                     /translation="MDPLAHHLLTQAYNNAWANHRLLAACAQLGAAEFAAPRTGFFPS
                     IRATLNHNLTVDWFYIDALERSLAGRPVNPAARAFFEPEQPCATCAELQPAQAASDRR
                     LIALCEALDGRALRCEIAVKRSAGIVHEPLPRLLAHLFQHQVHHRGQAHAMLSGTPIA
                     PPQLDDFFCANDAPVRAADFAALGFSEAVIWPAE"
     gene            1773..2282
                     /locus_tag="VNU48_14590"
     CDS             1773..2282
                     /locus_tag="VNU48_14590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWI12559.1"
                     /translation="MTPPLRAVAPSFSTRDFRAALGMFATGVTIVTARDSQGAPVGLT
                     ANSFNSVSLSPPLVLWSLARSAGSMPAFERGSHYAINILAADQHLLAERFASKAADRF
                     EGVAFREGAGGAPILDGSAAVFECFNRSRYEEGDHVIFVGEVERCHRRAGAQPLIFHG
                     GRYFTELPL"
     gene            complement(2258..>3073)
                     /locus_tag="VNU48_14595"
     CDS             complement(2258..>3073)
                     /locus_tag="VNU48_14595"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009553755.1"
                     /note="AcsA; in Sinorhizobium meliloti this enzyme is
                     required for acetoacetate activation; similar to
                     acetyl-CoA synthase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HWI12560.1"
                     /translation="VYEHVRKIDGRDIWLTPMSGGTDFAGAFIAGLPTLPVVEGEMQC
                     RCLGADVHAWGEGGADLVDEVGELVCAKPMPSMPLYFWNDPDGRRLHDSYFDMYPRVW
                     RHGDWIRITPRGGAIIYGRSDATINRHGIRMGTAELYRAVEALPEVLDSLVVDLEYLG
                     RESYMPLFVVLRDGLVLDAALTRRLKDGIRTALTARHVPNEIFQVEAVPRTLSGKKME
                     LPVKKLLMGTPVEQVFKLDAMANAGCVEWFVAFAARRNGPAAPGSAQSGSSVK"
BASE COUNT          432 a         1101 c         1111 g          429 t
ORIGIN      
        1 ccggccgcat cgggcagcag gcggccgccg acgagctcga agccctgcgc gcgcaggtcg
       61 aacaccttca ccggcacatc gaggcgcttc gtcagccagc cgctcaggtg ttgctcttcc
      121 ttcgcggcaa cctcgaccgg gtgacgcacc tccggcacgt agaccgcgtg cgcgaccgcg
      181 gcgcgccgca cccaggccgg ctcggccgcg gcgagcgcag cagcaggcgc ctgcgcgcgc
      241 cacatcagac cggcgccgag cgcgccgccg agcgcgaaca ccgcgagcgc cgcggcccag
      301 cgctgccagc cggccccgac gccgggcgcc ttgcgcgaca gcagctgcgc cagcgccgcc
      361 ggcaccggct cgtcgagcac gcccgccagc cgcgcgcgca gcagctcgcg atcggccgcc
      421 cacaggcgca cgcgcgccgc gtcctcgggg ttggcctgga gccaggcctc gacctcggcg
      481 tggcgctgcg tgggcagttc gccgtcgagc caggcgtgca gcgtgaggtc gtcgatcgcg
      541 gggttcatgg ccggttcccg tcccggttca gacgacgcgt cgcagcgccg gcgccgcggc
      601 cggcgcgcgg ccgtcgagca gcgcgcgcag cgcgacgcga gctcgcgaga tgcgcgacat
      661 caccgtgccg atcggaatgc gcagcagttc ggcgcactcg gcgtacgaca gctgttcgag
      721 cgtgaccagc agcagcggct cgcgctgctc gaccggcagc cgcttcagcg cggcgaccag
      781 gtccagctgc aggccgatgt cggggctgtg cgcggcgtgg cgctcgtcga gcgcgccgaa
      841 ccggtcgccg ccgtcggcaa gcggcagccg ggcgtggcgg cgacgctcgt ccatgtaggc
      901 gttgtgcgcg atgctcaggg tccagaccag gatgtcacgg cgctggtcga actggtgcca
      961 gtgcgcgagc gcgcgttcga gcgcggtctg caccaggtcg tcggcggcgc tcgcgtcgaa
     1021 caccagcgag cgcgcatagc ggcgcaggcg cgggatcgcc ccgagcagct gctgacgaaa
     1081 ggcggcgggg gcgtccacgt cgggtgtacg cggcgccgcc gcgcgttatt ccgccggcca
     1141 gatcacggct tcgctgaagc ccagcgcggc gaaatcggcg gcacgcaccg gcgcgtcgtt
     1201 ggcgcagaag aaatcgtcga gctgcggcgg cgcgatcggt gttcccgaca gcatcgcgtg
     1261 cgcctggccg cggtgatgca cctggtgctg gaacaggtgc gcgagcaggc gcggcagcgg
     1321 ctcgtggacg atgccggcgc tgcgtttcac cgcgatctcg caacgcagcg cgcgaccatc
     1381 gagcgcctcg cacaacgcga tcaggcgccg gtcgctggcc gcttgcgcgg gctgcagctc
     1441 ggcgcaggtg gcgcacggct gttcgggctc gaagaatgcg cgcgccgcgg ggttgacggg
     1501 ccggcctgcc aggctgcgtt cgagcgcgtc gatgtagaac cagtcgaccg tcaggttgtg
     1561 gttcagggtc gccctgatcg acgggaaaaa accggtgcgc ggtgccgcga actcggccgc
     1621 gccgagctgc gcgcaggccg cgagcaggcg gtgattggcc caggcgttgt tgtaggcctg
     1681 ggtcagcaga tggtgggcga gcgggtccac ggtgcaaagt gtaggcgtca cgacgccgca
     1741 tcggcgcgcg gcgaatcccg gacaatgcgc gcatgactcc cccgcttcgc gccgtggcgc
     1801 ccagcttctc gacgcgcgac ttccgcgcgg cgctgggcat gttcgccacc ggcgtgacca
     1861 tcgtcaccgc acgcgacagc cagggcgcgc cggtcggcct gaccgcgaac tcgttcaact
     1921 cggtctcgct gtcgccgccg ctggtgctgt ggagcctggc gcgcagtgcc ggctcgatgc
     1981 cggcgttcga gcgcggctcg cactatgcga tcaacatcct cgcggccgat cagcacctgc
     2041 tggccgagcg cttcgccagc aaggcggccg atcgcttcga gggcgtggcg tttcgcgaag
     2101 gcgccggcgg cgcgccgatc ctcgacggct cggccgcggt gttcgagtgc ttcaaccgca
     2161 gccgctacga agagggcgat cacgtgatct tcgtcggcga ggtcgaacgc tgccaccgcc
     2221 gcgccggcgc gcagccgctg atcttccacg gcgggcgtta tttcaccgag ctgccgctct
     2281 gagccgagcc cggcgccgcc gggccgttgc ggcgggccgc gaacgcgacg aaccattcga
     2341 cgcagcccgc gttggccatc gcgtcgagct tgaacacctg ttccaccggc gtgcccatca
     2401 gcagcttctt gaccggcagc tccatcttct tgcccgacag cgtgcgcggc acggcctcga
     2461 cctgaaagat ctcgttcggc acgtggcgcg ccgtcagcgc ggtgcggatg ccgtccttca
     2521 gccgccgggt cagcgcggcg tcgagcacga gcccgtcgcg cagcaccacg aacagcggca
     2581 tgtagctctc gcggccgagg tactcgaggt cgacgaccag gctgtccagc acctcgggca
     2641 gcgcctcgac cgcgcggtac agctcagcgg tgcccatgcg gatgccgtgg cggttgatgg
     2701 tcgcgtcgct gcggccgtag atgatcgcgc cgccgcgcgg cgtgatgcgg atccagtcgc
     2761 cgtggcgcca gacacgaggg tacatgtcga agtagctgtc gtgcagacgc cgtccatccg
     2821 ggtcgttcca gaaatacagc ggcatcgacg gcatcggttt cgcgcagacg agttcgccga
     2881 cctcgtcgac cagatcggcg ccgccttcgc cccatgcgtg cacgtcggca cccaggcagc
     2941 ggcactgcat ctcgccttcc acaaccggca gcgtcggcag cccggcgatg aaggcgccgg
     3001 cgaagtcggt gccgccggac atcggggtca gccagatatc gcgaccgtcg atcttccgca
     3061 catgttcgta gac
//
LOCUS       DATMJB010000690         3515 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_825161, whole genome shotgun sequence.
ACCESSION   DATMJB010000690 DATMJB010000000
VERSION     DATMJB010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825161"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /gene="gspG"
                     /locus_tag="VNU48_14600"
     CDS             complement(<1..321)
                     /gene="gspG"
                     /locus_tag="VNU48_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HWI12561.1"
                     /translation="MTLASNRGATRAATAPARKARGFTLIELMVVLVIIGVLAALIVP
                     NVLERADDARVTAARTDVNNLMQALKLYKLDNQRYPSAEQGLSALVAKPSVGVIPQNW
                     KPYLD"
     gene            380..979
                     /locus_tag="VNU48_14605"
     CDS             380..979
                     /locus_tag="VNU48_14605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12562.1"
                     /translation="MLARLSAFVIWALVAATAVFWGLRLFVRAPAAPAYTVPVGDAAA
                     VRGDLTRLLGSAPVAAPSATAAPEASARFRLLGIVAPKYPGDEAARAGQGVALMAIDG
                     KMPKAYAVGARLDNDLVLQSVSLRTVSIASSTRGAPTITLQLPPPAAAATGTLPATGF
                     GGMPGLPTPPAPVPQYAPQGVPQPGTLAPVRPETTAPTQ"
     gene            complement(992..2065)
                     /locus_tag="VNU48_14610"
     CDS             complement(992..2065)
                     /locus_tag="VNU48_14610"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HWI12563.1"
                     /translation="MSVNRSLISATSNPLLEQALRQKLARRGETTGGLGELEALAVRL
                     GLIQNTLKPRFRTPQIVIFASDHGLAVEGLSVAGSQSTALLAHQLLSSQLPVAVFARI
                     QGLELSVVDCGIAESVNPHARLLARKIAHGTRNARVTAAMSVDQAHAAIRAGMEIADS
                     LVGNAVACAGIGVGSHESASLVLARLSGANLSDLLISGPDMNDSTLAHLLAVLQTAQA
                     RHKDATDPVEVLAAFGGFEMAMMVGLMLVAGSKRHLIVVDGMSACAALMVAARIAPAV
                     TDYCVYCRSHSHEGLDQALALFHASALLELGMESTDGTGATLAWPLVRSAAALLTEVA
                     EGEEAGPTHPAPMNSTAEPATPL"
     gene            complement(2175..>3515)
                     /gene="ilvA"
                     /locus_tag="VNU48_14615"
     CDS             complement(2175..>3515)
                     /gene="ilvA"
                     /locus_tag="VNU48_14615"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HWI12564.1"
                     /translation="KMVQLSPAQRERGVICASAGNHAQGVALAARRLGCQAMIVMPVT
                     TPQVKVDAVRSHGGDVVLFGESYSDAYLHALELEAEHGLTFVHPFDDPDVIAGQGTIA
                     MEILRQHQAPIDAIFVAIGGGGLISGVAAYIKSVRPEIRVIGVQTNDSDAMLRSVKAG
                     KRVQLHDVGLFADGTAVKLVGEETFRLARELVDEYIVVDTDAACAAIKDVFQDTRSIL
                     EPSGALGVAAIKQYVDTHHCEGKTFVAITSGANMNFDRLRFVAERAEVGEEREAMFAV
                     TIPEERGSFKRFCELLGPRAVTEFNYRISDAKQAHIFVGLATTRRAESTQLAKLFARH
                     GFATLDLTGDELAKEHVRHMVGGRGSLADDERLYRFVFPERPGALMRFLSSMDPGWNI
                     SLFHYRNQGADHGRILVGIQVPRADRKDFKAFLEGLAYPCEDETDNPVYRLFLR"
BASE COUNT          498 a         1194 c         1270 g          553 t
ORIGIN      
        1 gtcgaggtag ggcttccagt tctgcgggat cacgccgacg ctcggcttcg cgacgagtgc
       61 gctcaggccc tgctcggcgc tcggatagcg ctggttgtcg agcttgtaca gcttcaaggc
      121 ctgcatcagg ttgttgacgt cggtgcgggc agcggtgacg cgcgcgtcgt cggcacgctc
      181 gagcacgttc ggcacgatca gcgccgccag cacgccgatg atgaccagca ccaccatcag
      241 ctcgatcagc gtgaagccgc gcgccttgcg ggccggggcg gtggcggcgc gcgtcgcgcc
      301 gcggttggag gccagggtca tggaatcgtc ctgccggcgc aggggccgtg gttggagttc
      361 ggttgaatca taatcgcgca tgctcgcacg actctcggcg ttcgtcatct gggccctggt
      421 ggccgccacc gcggtgttct gggggttgcg actgttcgtg cgcgctccgg ccgcgccggc
      481 ctacacggtg ccggtcggcg acgcggccgc ggtgcggggc gacctgacgc gcctgctcgg
      541 ctccgcgccg gtggcggcgc cttcggcgac cgccgcacct gaagccagcg cacgtttccg
      601 cctgctgggc atcgtcgcac cgaagtaccc gggcgacgag gcggcgcgtg ccgggcaggg
      661 cgtggcactg atggcgatcg acggcaagat gcccaaggcc tacgccgtgg gcgcgcgtct
      721 cgacaacgac ctcgtgctgc aatcggtcag cctgcgcacc gtgtcgatcg cgtcgtcgac
      781 gcgtggcgcc ccgacgatca cgctccaact cccgccgccc gcggcagccg ccacgggcac
      841 cttgccggcg accggtttcg gcggcatgcc ggggctgccg accccgccgg cgccggtgcc
      901 gcagtacgcg ccgcaggggg tgccgcaacc cggcacattg gccccggtgc gaccggaaac
      961 gacggctccg acgcagtagc cgggcgacaa ctcagagcgg tgtggccggt tcggccgtgc
     1021 tgttcatcgg cgccgggtgt gtcgggccag cctcttcacc ctcggccact tcggtgagca
     1081 gcgctgcggc gctgcgcacc agcggccagg ccagcgtggc gccggtgccg tcggtgctct
     1141 ccatgccgag ctcgagcagc gccgaggcgt ggaacagtgc cagcgcctgg tcgaggcctt
     1201 cgtggctgtg gctgcggcaa tagacgcagt agtcggtcac cgccggcgca atgcgcgccg
     1261 cgaccatcag tgccgcgcag gccgacatgc cgtcgacgac gatcaggtgg cgcttgctgc
     1321 cggccacgag catcaggccg accatcatcg ccatctcgaa cccgccgaat gcggccagca
     1381 cctcgaccgg atcggtcgcg tccttgtggc gtgcctgggc ggtctgcagc accgccagca
     1441 ggtgcgccag ggtcgagtcg ttcatgtcgg ggccggagat cagcaggtcg ctcaggttcg
     1501 cgcccgacag gcgcgccagc accagggatg cgctttcgtg cgagcccacg ccgatgccgg
     1561 cgcaggccac cgcattgccg accagcgaat cggcgatttc catgccggcg cggatcgccg
     1621 cgtgcgcctg gtcgaccgac atcgccgcgg tcacgcgggc gttgcgcgtg ccgtgcgcga
     1681 tcttgcgtgc gagcagccgc gcgtgcgggt tcaccgactc ggcgatgccg cagtcgacga
     1741 ccgacagctc cagcccctgg atgcgcgcga acaccgccac cggcagctgc gagctcagca
     1801 gctggtgcgc gagcagtgcg gtcgactggc tgccggccac gctcaggcct tcgacggcca
     1861 ggccgtggtc ggacgcgaag atcacgatct gcggcgtgcg aaagcgcggc ttcagcgtgt
     1921 tctggatcag cccgagccgc accgcgagcg cttcgagttc gcccaggccg ccggtggtct
     1981 cgccacggcg cgcaagcttc tggcgcagcg cctgttcgag cagcgggttc gaggtggcgg
     2041 agatgagcga gcgattgacc gacatggatt tccgtggatt cgacgaggga actcaaactc
     2101 gccacgattg tgaggcccga ggcggcctgc atgcaatcga cgcgcgtggc ggtgtgcgcg
     2161 ccgacgcctg cctgtcagcg caggaagagg cgataaacgg ggttgtccgt ctcgtcctcg
     2221 caggggtagg ccaggccttc gaggaaggcc ttgaagtcct tcctgtcggc gcgcggcacc
     2281 tggatgccga cgaggatccg gccgtggtct gcgccctggt tgcggtagtg gaacaggctg
     2341 atgttccagc ccggatccat gctcgacagg aagcgcatca gcgcgcccgg ccgctcgggg
     2401 aagacgaagc ggtacagccg ctcgtcgtcg gccaggctgc cgcggccgcc gaccatgtgg
     2461 cgcacgtgtt ccttggcgag ctcgtcgccg gtcaggtcga gcgtggcgaa gccgtggcgc
     2521 gcgaagagct tcgcgagctg ggtcgattcg gcgcggcggg tcgtggccag gccgacgaag
     2581 atgtgcgcct gcttcgcgtc cgagatgcgg tagttgaact cggtcacggc gcgcgggccg
     2641 agcaactcgc aaaagcgctt gaagctgccg cgctcctcgg gaatggtcac cgcgaacatc
     2701 gcctcgcgtt cctcgccgac ctcggcgcgc tcggcgacga agcgcagccg gtcgaagttc
     2761 atgttggcgc cggaggtgat cgcgacgaag gtcttgccct cgcagtggtg cgtgtcgacg
     2821 tactgcttga tcgcggccac gccgagcgcg cccgagggct cgaggatgct gcgggtgtcc
     2881 tggaacacgt ccttgatcgc ggcgcaggcg gcgtcggtgt cgacgacgat gtactcgtcg
     2941 accaattcgc gcgcgaggcg gaaggtctct tcgccgacga gcttgacggc ggtgccgtcg
     3001 gcgaacaggc cgacgtcgtg caactgcacg cgcttaccgg ccttcaccga gcgcagcatc
     3061 gcgtccgagt cgttggtctg cacgccgatc accctgatct cggggcgcac cgacttgatg
     3121 taggcggcca cgcccgagat cagcccgccg ccgccgatgg cgacgaagat cgcgtcgatc
     3181 ggggcctggt gctggcgcag gatctccatc gcgatcgtgc cctggccggc gatcacgtcc
     3241 gggtcgtcga acggatgcac gaaggtcagg ccgtgctcgg cttcgagttc gagggcatgc
     3301 aggtaggcgt cggagtagct ctcgccgaac agcaccacgt cgccgccatg cgagcgcacc
     3361 gcgtcgacct tgacctgcgg cgtggtcacc ggcatcacga tcatcgcctg gcaacccagg
     3421 cgcctggcgg ccagcgcgac gccctgggcg tggttgccgg ccgatgcgca gatcacgccg
     3481 cgttcgcgct gcgcggggct gagctgcacc atctt
//
LOCUS       DATMJB010000691         2854 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_212781, whole genome shotgun sequence.
ACCESSION   DATMJB010000691 DATMJB010000000
VERSION     DATMJB010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212781"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VNU48_14620"
     CDS             <1..791
                     /locus_tag="VNU48_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWI12565.1"
                     /translation="RHKPALIAKIERSEAIPALESIIRASDGIMVARGDLAVEVGNAA
                     VPALQKRMIKMAREMDRVVITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSA
                     ETAAGKYPVETIEMMASICVEAENAEEIALDASFTNKHFARIDQSIAMGALFTAYHLG
                     CKAILALTESGSTALWMSRSKIHVPIYGLTSQVRSQRRMALYRNVTPMLMPNYADRDE
                     ALAKAEALLVENGVLNPGDTYAITCGEPMGHPGGTNMLKVCRVA"
     gene            818..2032
                     /locus_tag="VNU48_14625"
     CDS             818..2032
                     /locus_tag="VNU48_14625"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HWI12566.1"
                     /translation="MTTSTLTLADIQAAALRLRGEVVDTPCMPSRTLSALAGCEVFLK
                     FENLQFTASFKERGALNKMAQLTAAERASGVLAVSAGNHAQGVAYHAQRMGIPATIVM
                     PRFAPAVKVERTRGFGASVVLEGDTFDDARAHGLRLAQERGMTLVHPYDDLAVMAGQG
                     TIALEMLQQQPQIDTLVVAIGGGGLIAGVATAARGLRPDIEVIGVQTERFPAAWNAKH
                     GGQRECAQATIADGIAVKAPGVLTLPLIRSLVDDVLLVGEDDIEQAILMLLEIEKTVV
                     EGAGGVGLAALMKHGARFRGRKVGLILCGGNIEPLVLAEIIERGMVKSGRLARLRLDV
                     RDVPGALANVATLLGKLGANIDEVQHQRAFSSLSVERAQIEVVVQTRGVVHIEQILSA
                     MRAEGYRAERVG"
     gene            2099..>2854
                     /locus_tag="VNU48_14630"
     CDS             2099..>2854
                     /locus_tag="VNU48_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HWI12567.1"
                     /translation="MPLVSMRQLLDHAATNGYGIPAFNVNNLEQVQAVMSAAAEVGAP
                     VILQASAGARKYAGEPFIKHLIQAAVESWPHIPLVMHQDHGQSPEVCKGAIELGFSSV
                     MMDGSLQADGKTIASFDYNVDVTRKVVDMAHKLGVTVEGELGCLGSLETMRGDKEDGH
                     GTESTMTMEQLLTDPEEAADFVKRTQLDALAIAIGTSHGAYKFTRKPTGDILAIGRIK
                     EIHQRIPNTHLVMHGSSSVPQDLLAVIREYGGKD"
BASE COUNT          501 a          943 c         1011 g          399 t
ORIGIN      
        1 ggcgtcacaa gccggcgctg atcgcgaaga tcgagcgcag cgaagcgatt cccgcgctcg
       61 agtccatcat tcgcgcgagc gacggcatca tggtcgctcg cggcgacttg gcggtcgagg
      121 tgggcaacgc ggccgtgccg gcgctgcaga aacgcatgat caagatggcg cgcgagatgg
      181 accgcgtggt catcaccgcg acacagatga tggagtcgat gatcgtcaac cccgtgccca
      241 cgcgcgccga ggtcagcgat gtggccaacg cggtgctcga tggcaccgac gcggtgatgc
      301 tcagcgccga gacggccgcg ggcaaatacc cggtcgagac gatcgagatg atggccagca
      361 tctgcgtcga ggccgagaac gcagaagaga tcgcgctcga cgcgagcttc acgaacaagc
      421 acttcgcgcg catcgaccag tcgatcgcga tgggggccct cttcaccgcg taccacctgg
      481 gctgcaaggc gatcctggcg ctgaccgagt cgggctcgac ggcgctgtgg atgagccgca
      541 gcaagatcca cgtgccgatc tacggcctga cctcgcaggt gcgcagccag cgccgcatgg
      601 cgctgtaccg aaatgtcacg ccgatgctga tgcccaacta cgccgaccgc gacgaggcgt
      661 tggcgaaggc cgaggcgctg ctggtcgaga acggcgtgct gaaccccggc gacacctacg
      721 cgatcacctg cggcgaaccg atgggccacc cgggcggcac caacatgctg aaggtctgcc
      781 gggtggcgtg agtcggcgct cgcgtgacaa tgcgcggatg accacgtcaa cgctgaccct
      841 ggcggacatc caggccgcgg ccctgcgcct gcgcggcgag gtcgtcgaca cgccgtgcat
      901 gccctcgcgc accttgtcgg cgctggccgg ctgcgaggtg ttcctgaagt tcgagaacct
      961 gcagttcacc gcctcgttca aggagcgcgg tgcgctcaac aagatggcgc agctgaccgc
     1021 ggccgagcgc gccagcggcg tgctcgcggt ctcggccggc aaccacgcgc agggcgtggc
     1081 gtaccacgcg cagcgcatgg gcatcccggc gacgatcgtg atgccgcgct tcgcgccggc
     1141 cgtgaaggtg gaacgcacgc gcggcttcgg tgccagcgtc gtgctcgagg gcgacacctt
     1201 cgacgacgcg cgcgcgcacg gcctgcgtct cgcgcaggag cgcggcatga cactggtgca
     1261 cccgtacgac gacctcgcgg tgatggccgg gcagggcacg atcgcgctgg agatgctgca
     1321 gcagcagccg cagatcgaca cgctggtggt ggcgatcggc ggcggcggcc tgatcgccgg
     1381 cgtcgcgacc gcggcgcgcg ggctgcggcc cgacatcgag gtgatcggcg tgcagaccga
     1441 gcgcttcccg gcggcctgga acgccaagca cggcgggcag cgcgagtgcg cgcaggcgac
     1501 catcgccgac ggcatcgcgg tcaaggcgcc cggcgtgctg acgctgccgc tgatccgcag
     1561 cctcgtcgac gacgtgctgc tggtcggtga ggacgacatc gagcaggcga tcctgatgct
     1621 gctcgagatc gagaagaccg tggtcgaggg cgccggcggc gtcggtctgg ccgcgctgat
     1681 gaagcacggc gcgcgcttcc ggggccgcaa ggtcgggctg atcctgtgcg gcggcaacat
     1741 cgagccgctg gtgctggccg agatcatcga gcgcggcatg gtcaagtcgg gccggctggc
     1801 gcggctgcgc ctggacgtgc gcgacgtgcc gggcgcgctc gcgaacgtcg ccacgctgct
     1861 cggcaagctc ggcgccaaca tcgacgaggt gcagcaccag cgcgcgttct cgtcgctgtc
     1921 ggtcgagcgc gcgcagatcg aggtcgtggt gcagacgcgc ggtgttgtgc acatcgagca
     1981 gatcctgagc gcgatgcgtg ccgaaggtta ccgcgccgag cgggtcggct gactacaatt
     2041 cgcaggatca cggcgcgatt ccacgcaccg ccatccactc gaccctcagg agaccgccat
     2101 gcccctcgtt tcgatgcgtc agttgctgga ccatgccgcc acgaacggtt atggcatccc
     2161 cgcgttcaac gtcaacaacc tcgagcaggt gcaggccgtg atgagcgcgg ccgccgaggt
     2221 cggcgcgccg gtgatcctgc aggccagtgc gggcgcgcgc aagtacgccg gcgagccctt
     2281 catcaagcac ctgatccagg ccgcggtcga atcgtggcca cacattccgc tggtgatgca
     2341 ccaggatcac gggcagagcc ccgaagtctg caagggcgcg atcgaactgg gcttcagctc
     2401 ggtgatgatg gacggctcgc tgcaggccga cggcaagacg atcgccagct tcgactacaa
     2461 cgtcgacgtg acgcgcaagg tcgtcgacat ggcgcacaag ctgggcgtga cggtcgaagg
     2521 cgaactcggc tgcctgggtt cgctcgagac catgcgcggc gacaaggaag acggccacgg
     2581 caccgagtcg acgatgacga tggagcagct gctgaccgac cctgaagagg cagcggactt
     2641 cgtcaagcgc acgcagctcg atgcgctcgc gattgcgatc ggcaccagcc atggcgccta
     2701 caagttcacg cgcaagccga ccggcgacat cctggcgatc gggcgcatca aggaaatcca
     2761 ccagcgcatc ccgaacacgc acctggtgat gcacggctcg agcagcgtgc cgcaggacct
     2821 gctcgcggtg atccgcgagt acggcggcaa agat
//
LOCUS       DATMJB010000692         3406 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_809894, whole genome shotgun sequence.
ACCESSION   DATMJB010000692 DATMJB010000000
VERSION     DATMJB010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809894"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VNU48_14635"
     CDS             <1..194
                     /locus_tag="VNU48_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWI12568.1"
                     /translation="WTFYAPAAEIGPGEALGRFRTGAAGLIVGADGHSRISYPDYAQA
                     FVDEIETPRFVRQIATAAY"
     gene            281..2335
                     /locus_tag="VNU48_14640"
     CDS             281..2335
                     /locus_tag="VNU48_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HWI12569.1"
                     /translation="MYALFEDAGKFLAGRVMTEADTSMQIELDSGKRVKVKSANVLLK
                     FDQPQPAELIASAQAIARDIDLDLAWEFAPEAEFSFGDLAREYFEPGAGIAKQAGALF
                     RLFEAPHYFRRLGKGNFRKAPEEIVKAALLGIERKKQIAAQIDAWAGELVLGQCPAPV
                     REQLYKLLFKPDKNAPEYKAVVEAARRAQKAPLDLLKGAGAIDSPYQFHWRRFLFESF
                     PKGTGFPALQAPAIREALPLAPVRAFSIDDSATTEIDDALSVQGLGSGAVTFGIHIAA
                     PGLAFAPDAPLDKIARERLSTVYMPGHKLTMLPDEVVQAYTLIAGRECPAVSLYLTLD
                     ETTLAITRSETRLERVPIVANLRHDQLDGVITEASLTGDAPAAYEFAPELAFAFRLAR
                     HLKAQREVVRGKPENFNRPDYSFRLEGIEGSEPQGSERVLISTRQRGSPLDLIVAEAM
                     ILANSTWGAWLAECGVPGIYRSQASMAPGVKVRMGTKPAPHAGMGVAQYTWATSPLRR
                     YVDLVNQWQIIACARHGRTAALAAPFKPKDVQLFAIISAFDSAYAEYNGFQQGIERYW
                     ALRSLAQDGITELDAVAMKDGLVRADHLPLVFRAAGADSLPRGAHVRVRVTGTDLLTL
                     DLHANVVARLDDVAADAAPADADADEGAESAGPLTLAIDVAGDGAADTDATAAAADAP
                     AA"
     gene            2341..3201
                     /locus_tag="VNU48_14645"
     CDS             2341..3201
                     /locus_tag="VNU48_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HWI12570.1"
                     /translation="MLRKLSTLQIALIVSIAVHAALLTVRIVDPEGFNRIFQDTPLEV
                     ILVNSRSGEPPEKAQAIAQAALAGGGEAAEGRATSPLPPSALVEVGESNDEAHKRIEQ
                     LQETQQQLLAQIRRELALLPAPDPQRDQGNPEEQAQEERRRQLVQLLAEIEKRVNEEN
                     ARPKKRYISPSTREEVYAMYYDRLRRKIEERGTRNFPENQGRKLYGELTMNVTVDAEG
                     RVIDTEIVLPSKSRVLDRRAVAIVRAAAPFGPFSAAMRKRADQLVITSRFKFTREDGL
                     ETTLSATPTK"
     gene            3210..>3406
                     /gene="aroE"
                     /locus_tag="VNU48_14650"
     CDS             3210..>3406
                     /gene="aroE"
                     /locus_tag="VNU48_14650"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010813194.1"
                     /note="AroE; catalyzes the conversion of shikimate to
                     3-dehydroshikimate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWI12571.1"
                     /translation="MDRYVVAGNPVEHSQSPFIHTEFARQTAQAMRYERLLVPLDGFA
                     DAIRALAGAGAAGCNVTVPFK"
BASE COUNT          559 a         1222 c         1177 g          448 t
ORIGIN      
        1 actggacctt ctacgccccc gccgccgaga tcggcccggg cgaagcgctg gggcgcttcc
       61 gcaccggtgc ggccggcttg atcgtcggcg ccgacggcca cagccggatc tcgtaccccg
      121 actacgcgca ggccttcgtc gacgaaatcg aaacgccgcg cttcgtgagg cagatcgcca
      181 ccgccgccta ctgaagaagc cgaagcggcg cgggcacgcg gcgtcgtgcg cgcgccgacc
      241 gcggaggcag cgtggggcaa agcggcgaca atcacgcccc atgtatgccc tgttcgaaga
      301 cgccggaaaa ttcctcgccg gccgcgtgat gaccgaagcc gacacctcga tgcagatcga
      361 gctcgactcg ggcaagcgcg tcaaggtcaa gtccgccaac gtgctgctca agttcgacca
      421 gccgcagccg gccgagctga tcgcctcggc gcaggcgatc gcgcgcgaca tcgacctcga
      481 cctggcctgg gagttcgcgc ccgaggccga gttcagcttc ggcgacctgg cgcgcgagta
      541 cttcgagccc ggcgccggca tcgccaagca ggccggcgcg ctgttccgcc tgttcgaggc
      601 gccgcactac ttccggcgcc tcggcaaggg caacttcagg aaggcgcccg aagagatcgt
      661 caaggccgcg ctgctgggca tcgaacgcaa gaaacagatc gccgcgcaga tcgatgcctg
      721 ggccggcgaa ctcgtgctgg ggcaatgccc ggcgccggtg cgcgagcagc tgtacaagct
      781 gctgttcaag cccgacaaga acgcacccga gtacaaggcg gtggtcgagg ccgcgcggcg
      841 cgcgcagaag gcaccgctcg atctgctgaa gggcgccggg gcgatcgact cgccctacca
      901 gttccactgg cgccgcttcc tgttcgagag cttcccgaag ggcaccggct tcccggcgct
      961 gcaggcgccg gcgatcaggg aagcgctgcc gctcgctccc gtgcgcgcgt tctcgatcga
     1021 cgactcggcg acgaccgaga tcgacgatgc gctgtcggtg caggggttgg gcagcggcgc
     1081 cgtcaccttc ggcatccaca tcgccgcacc cggcctggcg ttcgcgcccg acgcgccgct
     1141 ggacaagatc gcgcgcgaac gcctgtcgac cgtctacatg cccggccaca agctgacgat
     1201 gctgcccgac gaggtggtgc aggcctacac gctgattgcg gggcgcgaat gcccggccgt
     1261 ctcgctgtac ctgacgctgg acgagacgac gctggcgatc acccgcagcg agacccggct
     1321 cgagcgcgtg ccgatcgtcg cgaacctgcg ccacgaccag ctcgacggcg tgatcaccga
     1381 agccagcctg accggcgacg cgccggcggc ctacgaattc gcgcccgagc tggcattcgc
     1441 gtttcgcctt gcgcgccatc tgaaggcgca gcgtgaggtc gtgcgcggca agccggagaa
     1501 cttcaaccgg cccgactaca gcttccggct ggagggcatt gagggcagcg aaccgcaggg
     1561 cagcgagcgc gtgctgatct cgacgcgcca gcgcggctcg ccgctggacc tgatcgtcgc
     1621 cgaggcgatg atcctggcca acagcacctg gggcgcctgg ctcgccgagt gcggcgtgcc
     1681 cggcatctac cgcagccagg ccagcatggc gccaggcgtg aaggtgcgca tgggtaccaa
     1741 gccggcgccg cacgccggca tgggcgtggc gcagtacacc tgggcgacct cgccgctgcg
     1801 gcgctatgtc gacctcgtga accagtggca gatcatcgcc tgcgcacgtc acggccgcac
     1861 cgcggcgctg gccgcgccgt tcaagccgaa ggacgtgcag ctgttcgcga tcatctcggc
     1921 cttcgactcg gcctacgccg agtacaacgg cttccagcag ggcatcgagc gctactgggc
     1981 gctgcgctcg ctggcgcagg acggcatcac cgaactcgac gccgtggcga tgaaggacgg
     2041 cctggtgcgc gccgaccacc tgccgctggt gttccgcgcc gcgggcgccg acagcctgcc
     2101 gcgcggcgcg catgtgcgcg tgcgcgtcac cggcaccgac ctgctgacgc tggacctgca
     2161 tgcgaacgtc gtcgcccgtc tcgacgacgt ggcggccgat gcggcaccgg ccgatgccga
     2221 tgccgacgag ggggccgaga gcgcgggccc gctgacgctg gcgatcgatg tcgccggcga
     2281 cggtgccgcc gacaccgacg cgaccgcggc cgccgccgac gcgcctgccg cctgagcccg
     2341 atgctgcgca agctctcgac cctgcagatc gcgctgatcg tctcgatcgc ggtccacgca
     2401 gcgctgctga cggtgcgcat cgtcgacccc gaaggcttca atcgcatctt ccaggacacg
     2461 ccgctcgaag tgatcctggt caactcgcgc tccggcgagc cgcccgagaa ggcccaggcg
     2521 atcgcacagg cggcgctggc cggcggcggc gaggcggccg agggccgggc cacctcgccg
     2581 ctgccgccgt cggcgctggt cgaggtcggc gagtccaacg acgaggcgca caagcgcatc
     2641 gagcaattgc aggagacaca gcagcagttg ctggcgcaga tccgccgcga gctcgcgctg
     2701 ctgccggcac ccgacccgca acgcgaccag gggaatccgg aggagcaggc ccaggaggaa
     2761 cgccgccgcc agctcgtgca gctgctggcc gagatcgaga agcgcgtcaa cgaggaaaac
     2821 gcgcgcccga agaagcgcta catcagcccg tccacgcgcg aggaggtcta cgcgatgtac
     2881 tacgaccgcc tgcgtcgcaa gatcgaggag cgcggcacgc gcaacttccc ggagaaccag
     2941 ggtcgcaagc tgtatggcga gctgacgatg aacgtcacgg tcgacgccga ggggcgcgtg
     3001 atcgacaccg agatcgtgct gccgtcgaag tcgcgcgtgc tcgaccggcg cgcggtcgcg
     3061 atcgtgcgcg cggccgcgcc gttcgggccg ttcagcgcgg cgatgcgcaa gcgcgccgac
     3121 cagctggtga tcacctcgcg cttcaagttc acgcgcgagg acgggctgga aacgacgctc
     3181 agcgccacgc ccacgaagtg accgtgacga tggaccgcta cgtcgtggcc ggcaacccgg
     3241 tcgagcacag tcagtcgccc ttcatccaca ccgagttcgc gcgccagacc gcgcaggcga
     3301 tgcgctacga gcgcctgctc gtcccgctcg acggtttcgc cgacgcgatc cgcgcgctgg
     3361 ccggcgccgg cgccgccggc tgcaacgtca cggtgccgtt caagtt
//
LOCUS       DATMJB010000693         3930 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_783212, whole genome shotgun sequence.
ACCESSION   DATMJB010000693 DATMJB010000000
VERSION     DATMJB010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783212"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="VNU48_14655"
     CDS             complement(<1..1918)
                     /locus_tag="VNU48_14655"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HWI12572.1"
                     /translation="MNARPHHITRYTDWLARERGLRFDATTTEGYDALWRWSVGDLRA
                     FWGSIWDFFGLHSPTPTTSVLEAETMPGARWFVGAQVNYAQHLLSHADAAHAAGHPAI
                     VFQNEALRATGSRGELQEISWPELRRQVASLAAELRRLGVQRGDRVGAFMPNIPATVV
                     AFLACASLGAIWSVCSPDMGPIAVLDRFRQIEPTLLIACDGYTYGGIDHDRMPVLRDL
                     LAELPSVRDVLLLKYQSAGADAHSLAAAQRAVHDFAAATAHDVPFEPVWLPFDHPVWI
                     VYSSGTTGLPKPIVHGHGGAMVEALKAGALHNDVGPSVLTGDRYHWYSTTGWIMWNAQ
                     IGALLGGTTICIFDGNPGGAALASGQAPDLTTLWRFAADAGVTFFGAGAAFFANCLKA
                     GVEPMKVADLSALRAVGSTGSPLSVESYQWVYDQLPKVDGHDIWLNPISGGTDFAGAF
                     VAGMSTLPTVIGEMQCRCLGADVHAWDEQGRELIDEVGELVCAKPMPSMPLYFWNDSG
                     NRRYHDSYFDVYPGVWRHGDWIKITPRGGAIIYGRSDATINRHGIRMGTAELYRAVEA
                     LPEVLDSLVVDLEYLGRESYMPLFVVLRDGLVLDEALSRRIKEGIKTALSARHVPNEI
                     FQVSAVPRTLSGKKM"
     gene            complement(2025..3833)
                     /locus_tag="VNU48_14660"
     CDS             complement(2025..3833)
                     /locus_tag="VNU48_14660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI12573.1"
                     /translation="MIDRRTWSAALLGTLALPSIARASDAGGGNILRVPFESAETGFD
                     PAQISDLYSARVTAHIFEALYHYDWLAMPVKVRPLTAQSLPEIADDFRVWTVRVRPGI
                     YFADDPAFKGRKRELVAEDYVYAIKRMFDPAMKSPSLSSLEEEGIVGVDALRETALRE
                     RKPFDYDTPIEGLRALDRYTLRFRLREPRPRFVATLCSSSIMGAVAREVVEHYGDEIM
                     AHPVGTGPYRLKYWRRSSRIELDRNPGFREMLYDAEPAADDVQGQAWLARFKGRRLPL
                     NDGVEIAVLEEGQARWLSFLNGQVDYVRVPPEFTNYAAPGGTLAPNLAKRGILARKYV
                     NPDFTMSWFNMEDPVVGGYTPDKVALRRAVGLAYNIDREIRIVRRGSAIAAQAPMPPG
                     TYGYDRAFRSEQSAYDPARAKALLDLFGYVDRNGDGWREQPDGAPLVLHRATQTSAIE
                     RQLDENWQKSCAAIGVRMVFQTAQWPENFKAARAGKLQMWGLASTLGEDAQPGFEYMY
                     GPSSGSGNLARFKLPAFDAIYRRMLALPDGPERAALFLEAAKLLTAFMPYKMHVHRVY
                     TDLTQPWVTGWRQGLFRNESWQYAEVDAGLRARLAA"
BASE COUNT          597 a         1350 c         1384 g          599 t
ORIGIN      
        1 ccatcttctt gcccgacagc gtgcgcggca ccgcgctgac ctggaaaatc tcgttcggca
       61 cgtggcgcgc cgacagcgcc gtcttgatgc cctctttgat gcgccggctc agtgcttcat
      121 cgagcacgag gccgtcgcgc agcacgacga acagcggcat gtagctttcg cggccgaggt
      181 attcgaggtc gacgacgaga ctgtcgagca cctcgggcaa ggcctcgacc gcgcggtaca
      241 actcggccgt gcccatgcgg atgccgtggc ggttgatcgt cgcatccgag cggccgtaga
      301 tgatcgcgcc gccgcgcggc gtgatcttga tccagtcgcc gtggcgccag acgcccgggt
      361 acacgtcgaa gtagctgtcg tggtagcgcc gattgccgga gtcgttccag aagtacagcg
      421 gcatcgacgg catcggcttc gcgcagacca gctcgccgac ttcgtcgatg agctcgcggc
      481 cctgctcgtc ccaggcgtgc acgtcggcgc ccaggcagcg gcactgcatc tcgccgatca
      541 cggtcggcag cgtcgacatg ccggcgacga acgcgccggc gaaatcggtg ccgcccgaga
      601 tcgggttcag ccagatgtcg tgcccgtcga ccttcggcag ctggtcgtag acccactggt
      661 aggactcgac cgagagcggc gagccggtcg agccgaccgc acgcagggcc gacaggtcgg
      721 cgaccttcat cggttcgacg ccggccttca ggcagttggc aaagaacgcg gcaccggcgc
      781 cgaagaaggt cacgccggcg tcggccgcga agcgccacaa ggtcgtcagg tccggcgcct
      841 ggcccgacgc aagagcggcg ccacccgggt tgccgtcgaa gatgcagatc gtcgtgccgc
      901 cgagcagcgc gccgatctgc gcgttccaca tgatccagcc ggtcgtgctg taccagtggt
      961 agcggtcgcc ggtcagcacg ctcgggccga catcgttgtg cagcgcgccg gccttcagcg
     1021 cctcgaccat cgcaccgccg tggccgtgca cgatcggctt cggcagcccg gtggtgccgc
     1081 tcgaatagac gatccacacc ggatggtcga agggcagcca caccggctcg aacggcacgt
     1141 cgtgcgcggt cgcggcggcg aagtcgtgaa ccgcgcgctg cgctgcggcg aggctgtgcg
     1201 catcggcacc ggcgctctgg tacttcagca gcagcacatc gcgcacgctc ggcaattcgg
     1261 cgagcaggtc gcgcagcacc ggcatgcggt cgtgatcgat gccgccgtag gtgtagccgt
     1321 cgcaggcgat cagcagtgtc ggctcgatct ggcggaaacg gtcgagcacc gcgatcggcc
     1381 ccatgtcggg cgagcacacc gaccagatcg cacccaggct cgcgcaggcc aggaatgcga
     1441 caacggtggc cgggatgttg ggcatgaacg cgccgacccg gtcgccacgc tgcacgccca
     1501 ggcgccgcag ctcggccgcg agcgacgcga cctggcgacg cagctcgggc catgaaatct
     1561 cctgcagctc gccgcgcgag ccggtcgcgc gcagtgcctc gttctggaac acgatcgccg
     1621 ggtgcccggc ggcatgcgcc gcgtcggcgt gcgagagcag gtgctgcgcg tagttgacct
     1681 gggcgccgac gaaccagcgc gcaccgggca tcgtctcggc ctcgagcaca ctggtggtcg
     1741 gcgtcggcga gtgcagcccg aagaaatccc agatgctgcc ccagaacgcg cgcaggtcgc
     1801 cgaccgacca gcgccacagc gcgtcgtagc cttcggtcgt ggtcgcgtcg aagcgcaatc
     1861 cgcgctcgcg cgcgagccag tcggtgtagc gggtgatgtg gtgcggtcgt gcattcattt
     1921 caggtcggat tcatggttgt cgacgttgtc taacgcctgg ccggaccggc ccatgctagc
     1981 cgagcgccgc gcgccgcaca gcggggaatc cacggtgcag cgggtcaggc cgcgaggcgc
     2041 gcgcgcagcc ccgcgtcgac ctcggcgtac tgccacgatt cgttgcggaa caggccctgg
     2101 cgccagccgg tcacccaggg ttgcgtcagg tcggtgtaga cgcggtgcac gtgcatcttg
     2161 tacggcatga aggcggtcag gagcttggcc gcctcgagga acagcgcggc gcgctcgggg
     2221 ccatcgggca gcgccagcat gcggcggtag atcgcatcga acgcgggcag cttgaagcgc
     2281 gcgaggttgc cgctgccgct cgacgggccg tacatgtatt cgaagccggg ctgcgcgtcc
     2341 tcgccgagcg tcgaggccag cccccacatc tgcagcttgc cggcgcgcgc ggccttgaag
     2401 ttctcgggcc actgcgcggt ctggaacacc atgcgcacgc cgatcgccgc gcagctcttc
     2461 tgccagttct cgtcgagctg gcgttcgatg gccgaggtct gcgtcgccct gtgcagcacc
     2521 agcggcgcgc cgtcgggctg ctcgcgccag ccgtcgccgt tgcggtcgac atagccgaac
     2581 aggtcgagca gcgccttcgc gcgcgccggg tcgtacgcgc tctgctcgct gcgaaaggcc
     2641 cggtcgtagc cgtaggtgcc gggcggcatc ggcgcctgcg cggcgatcgc actgccgcgc
     2701 cgcacgatgc ggatctcgcg gtcgatgttg taggccaggc ccaccgcacg gcgcagcgcg
     2761 accttgtcgg gcgtgtagcc gcccacgacc gggtcttcca tgttgaacca cgacatcgtg
     2821 aagtccgggt tcacgtactt gcgcgcgagg atgccgcgct tggccaggtt cggcgcgagc
     2881 gtgccgccgg gcgcggcgta gttggtgaac tcgggcggca cgcgcacgta gtcgacctgg
     2941 ccgttcagga acgacagcca gcgcgcctgc ccttcctcga gcaccgcgat ctcgacgccg
     3001 tcgttgagcg gcaggcggcg gcccttgaag cgcgccagcc aggcctggcc ctgcacgtcg
     3061 tcggcagccg gttcggcgtc gtacagcatc tcgcgaaagc ccgggttgcg gtcgagctcg
     3121 atgcgcgaac tgcggcgcca gtatttcagc cggtacggcc cggtgcccac cgggtgcgcc
     3181 atgatctcgt cgccgtagtg ctcgacgacc tcgcgcgcca ccgcgcccat gatcgacgag
     3241 ctgcacagcg tcgcgacgaa gcgcggccgc ggctcgcgca ggcgaaagcg cagggtatag
     3301 cggtcgagcg cgcgcaggcc ctcgatcggc gtgtcgtagt cgaagggctt gcgctcgcgc
     3361 aacgccgtct cgcgcagcgc gtcgacgccg acgatgcctt cctcctcgag gctggacagg
     3421 ctcgggctct tcatcgccgg gtcgaacatg cgcttgatcg cgtagacata gtcctcggcg
     3481 acgagctcgc gcttgcggcc cttgaaggcc ggatcgtcgg cgaagtagat gccgggcctc
     3541 acacgcaccg tccagacgcg gaagtcgtcg gcaatttccg gcagcgattg cgccgtcagc
     3601 ggccgcacct tgaccggcat cgcgagccag tcgtagtggt acagcgcctc gaagatgtgt
     3661 gcggtgacgc gcgccgagta caggtcggag atctgcgccg ggtcgaagcc ggtctcggcc
     3721 gattcgaagg gcacgcgcag gatgttgccg ccgcctgcgt ccgatgcgcg cgcaatcgac
     3781 ggcagcgcca gcgtgcccag caacgcggcg gaccaggtgc ggcggtcgat catcgcggcg
     3841 cctccttcag cgctttggca cggccgacgg gtcgatgtcg acgtagtgcc accactcctg
     3901 ccagaacggc gtgcggcggt agccagatcg
//
LOCUS       DATMJB010000694         2294 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_36279, whole genome shotgun sequence.
ACCESSION   DATMJB010000694 DATMJB010000000
VERSION     DATMJB010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36279"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VNU48_14665"
     CDS             <1..701
                     /locus_tag="VNU48_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="teicoplanin resistance protein VanZ"
                     /protein_id="HWI12574.1"
                     /translation="FIGRSAGALALLLLWPVGLLFPLPVPLGQGQVLAKLQEGIAMLL
                     ADTSVAPWVAGWADAEVDRSALSPGGEFALIALGLLGPCLVAFSIARAGWRRLVLVLG
                     AASLGVLATSLSTALNFGPQHLMAWSTPQALAALAFAVVLASLLSVVPRRAAAGLGLM
                     ALTALVTVVAQAPSDPFFAQSLQGWEQGRFIRFYGVARWVGWLWPYAAIVYLLARLGA
                     RDEGAPTPAPKMPA"
     gene            698..1024
                     /locus_tag="VNU48_14670"
     CDS             698..1024
                     /locus_tag="VNU48_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWI12575.1"
                     /translation="MSYYQRHIFFCLNERDKGEDSCAPQGAQAGFDHCKKRIKGEQLA
                     GPGGVRVNKAGCLDRCAGGPVAVVYPEAVWYTYVDKADIDEIVDSHLKRGQVVERLLL
                     PADVGR"
     gene            1026..1652
                     /locus_tag="VNU48_14675"
     CDS             1026..1652
                     /locus_tag="VNU48_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWI12576.1"
                     /translation="MNSGTVRQTIAGPAGVIACAIDSPAPPARGVAVVCHPHPQFGGT
                     LDNKVVQTLARAFVALGWRSVRFNFRGVDGSAGAWDEGVGEIDDALAVIAALRAPGEA
                     LLLAGFSFGAYVASQAAVRLADDAKASRVVLVGPSTEKQQMANVPADTVVIHGESDEV
                     VPLAATLAWARPQSLPVIVFPGVGHFFHGQLGLLKNTVVQELQPRVPL"
     gene            1711..>2294
                     /locus_tag="VNU48_14680"
     CDS             1711..>2294
                     /locus_tag="VNU48_14680"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HWI12577.1"
                     /translation="MNRLLVLVFSLAFAATASAQALQPPEVAAKSFLVLDLTSNQTLA
                     ERDADASADPASLTKLMSLYVVFTAIHDKKLTLDQKLPVSKRAWDERKAGGSLMFIDT
                     TMTPTVEELLKGEIVQSGNDAAVVLAEAVGGTLDQFVAMMNRQAAAFGLKNTVFKNVT
                     GLTEAGHHSSARDLATIATHIIRDYPEFFAYYSI"
BASE COUNT          306 a          797 c          814 g          377 t
ORIGIN      
        1 ggttcatcgg gcgcagcgcc ggcgcgcttg cgctgctgtt gctgtggccg gtcggtctgc
       61 tgttcccgct gccggtgccg ctgggccagg gccaggtgct ggccaaactc caggaaggca
      121 tcgcgatgct gctggccgac acctcggtcg cgccctgggt cgcgggctgg gcggatgccg
      181 aggtcgatcg cagcgcgctg tcgcccggcg gcgagttcgc gctgatcgcg ctcggcctgc
      241 tcgggccgtg cctggtcgcg ttctcgatcg cccgcgccgg ctggcggcgt ctggtgctgg
      301 tgctgggcgc ggcctcgctc ggcgtgctcg ccacgagctt gtcgacggcg ctgaacttcg
      361 gcccgcagca cctgatggct tggagcacgc cgcaggcgct cgcagcactg gcgttcgcgg
      421 tcgtgctggc gtcgctgctc agcgtcgtgc cgcgccgcgc cgcggccggg ctcgggctga
      481 tggcgctgac cgcgctcgtg accgtggtgg cgcaggcgcc gtcggacccg ttcttcgcgc
      541 agagcctgca gggctgggag caggggcgtt tcatccgctt ctacggggtg gcgcgctggg
      601 tcggctggct ctggccgtat gcggcgatcg tctacctgct ggcgcgtctg ggcgcgcgcg
      661 acgagggcgc gccgaccccg gctcctaaaa tgccggcatg agctactacc agcgtcacat
      721 cttcttctgc ctcaacgagc gcgacaaggg cgaggactcc tgtgcgccgc aaggcgcgca
      781 ggccggcttc gaccactgca agaagcgcat caagggcgag cagctggccg gccccggcgg
      841 tgtgcgcgtc aacaaggccg gctgcctgga ccgctgcgcc ggcggcccgg tcgcggtggt
      901 ctaccccgaa gccgtctggt acacctatgt cgacaaggcc gacatcgacg agatcgtcga
      961 ttcgcacctg aagcgcgggc aggtcgtcga gcgcctgctg ctgccggcgg acgtgggccg
     1021 ctgacatgaa ctcgggcacc gtgcgccaga caatcgccgg gccggccggc gtgatcgcgt
     1081 gcgcgatcga ttcgccggca ccgcccgcgc gtggtgtcgc cgtggtctgc cacccgcacc
     1141 cgcagttcgg cggcaccttg gacaacaagg tcgtgcagac gctcgcccgt gctttcgtcg
     1201 cgctcggctg gcgcagcgtg cgcttcaact tccgcggcgt cgatgggtcg gccggcgcgt
     1261 gggacgaggg cgtcggcgag atcgacgacg cgctcgcggt gatcgccgcg ctgcgcgcgc
     1321 ccggcgaagc gctgctgctt gccggcttct cgttcggcgc ttacgtcgcg tcacaggccg
     1381 cggtgcgcct ggccgatgat gcgaaggcgt cgcgcgtggt gctggtcggg ccgtcgacgg
     1441 aaaagcagca gatggcgaac gtgccggcag acaccgtcgt gattcacggc gagagcgacg
     1501 aggtcgtgcc gctcgccgcg acgctggcct gggcccggcc gcagtcgctg ccggtgatcg
     1561 tgttccccgg cgtcgggcac ttcttccatg ggcagctcgg cctgctgaaa aacaccgtcg
     1621 tgcaggagct gcagccgcgc gtccctctct gagtcacagc catcgtcggc ggcgcgtgcg
     1681 ccggccgcat tcctcttcac cggatcatca atgaatcgcc ttctcgttct cgtcttctcg
     1741 ctcgcgttcg cggccaccgc gtcggcccag gccctgcaac cgcccgaggt cgcggccaag
     1801 agctttctgg tgctggacct gaccagcaac cagacgctgg ccgaacgcga cgccgatgcg
     1861 agcgccgacc cggcctcgct gaccaagctg atgagcctgt acgtcgtctt caccgcgatc
     1921 cacgacaaga agctcacgct cgaccagaag ctgccggtgt ccaagcgcgc ctgggacgag
     1981 cgcaaggccg gcggctcgtt gatgttcatc gacacgacga tgacgccgac cgtcgaggag
     2041 ctgctcaagg gcgagatcgt gcagtcgggc aacgacgccg cggtggtgct ggccgaggcc
     2101 gtcggcggca cgctcgacca gttcgtcgcg atgatgaatc gccaggcggc ggccttcggc
     2161 ctgaagaaca ccgtgttcaa gaacgtcacc ggcctgaccg aggccgggca ccacagcagc
     2221 gcgcgcgacc tggcgacgat cgcgacgcac atcattcgcg actaccccga gttcttcgcc
     2281 tattactcga tcaa
//
LOCUS       DATMJB010000695         2026 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_36406, whole genome shotgun sequence.
ACCESSION   DATMJB010000695 DATMJB010000000
VERSION     DATMJB010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36406"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VNU48_14685"
     CDS             complement(<1..207)
                     /locus_tag="VNU48_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWI12578.1"
                     /translation="MTEVLNAPVFADVPERAFQAGPAGTHITIRGLTKYFAGWPLYEN
                     FDLDIPKHAIVSVFGPNGCGKSTLI"
     gene            complement(210..1043)
                     /locus_tag="VNU48_14690"
     CDS             complement(210..1043)
                     /locus_tag="VNU48_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWI12579.1"
                     /translation="MGDTTTIVAAAKPWSGSTDASAASPAKFSWGRLLPFVGPVALFI
                     VWDLVVRAGLIKAILLPTPWATLQALVTGLAGGPLLADFAVTVLRTLEAFVLAALVGV
                     PLGVLLGSNEKAYRSVEFLIDFFRSTPSSALIPLFLLIFGVSDINKVAIAAFGALLIV
                     LFNSAYGVINARKQRVMAARVMGATRWQIFKDVLIWESLQPTFVGLRSAVSMALVIVI
                     VAEMFIGSDSGLGHRIIDAQQVLNVKSMYAAILAAGALGYALNVLFLVAERRIVHWSG
                     R"
     gene            complement(1047..>2026)
                     /locus_tag="VNU48_14695"
     CDS             complement(1047..>2026)
                     /locus_tag="VNU48_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWI12580.1"
                     /translation="TRRRLLGAAACGSALAVVGAPALVRAQAGPKIRVGYWPVAAGLP
                     FFAAIEKGYFKEAGLEVEPLKFAGAQQVMEAMLSGRSDGSANGTGSANLAIGEIAQPG
                     LFKIFATNPSNAKHVLEEFLLPKDSPIKTMAELKGKRVASGPGIQNVTLAKTMLERAG
                     AGAMSVTELPIGQHVAALAAGQIDAAYTLEPTGTIGRMNGTTRVLEAGVVAHYILGDP
                     MAPWHGGSASLTSEFIKKNPAEAKKFIAAYARGIELVRSKPEEARLFMKGYTAIEGAL
                     TAEVPLASYMLYSEFKASDVAYFQKFYDLFSDKGIFEKKVAVEPLLYKA"
BASE COUNT          324 a          691 c          697 g          314 t
ORIGIN      
        1 gatcagcgtc gacttgccgc agccattcgg gccgaacacc gagacgatcg cgtgcttcgg
       61 gatgtcgagg tcgaaattct cgtacagcgg ccagccggcg aaatacttcg tcagcccgcg
      121 gatcgtgatg tgcgtgccgg ccgggccggc ctggaacgct cgctcgggaa cgtccgcgaa
      181 caccggcgcg ttgagaacct cggtcatggc tacctcccgc tccagtggac gatgcggcgc
      241 tccgcgacca ggaacagcac gttcagcgcg tagccgagcg cgccggccgc gaggatcgcg
      301 gcgtacatgc tcttgacgtt cagcacctgc tgcgcatcga tgatgcggtg gcccaggccg
      361 gagtcggagc cgatgaacat ctcggcgacg atcacgatca ccagcgccat cgacaccgcg
      421 gagcgcaggc cgacgaaggt cggctgcagg ctctcccaga tcagcacgtc cttgaagatc
      481 tgccagcgcg tggcgcccat gacccgggcc gccatcacgc gctgcttgcg cgcgttgatc
      541 acgccgtagg cgctgttgaa cagcacgatc agcagcgccc cgaacgcggc gatcgcgacc
      601 ttgttgatgt ccgagacgcc gaagatcagc aggaacagcg ggatcagcgc cgaactcggc
      661 gtcgagcgga agaagtcgat caggaactcg acgctgcggt aggccttttc gttgctgccc
      721 agcagcacgc ccagcggcac gccgaccagc gcggcgagca cgaaggcttc gagcgtgcgc
      781 agcaccgtga ccgcgaagtc ggccagcagc ggcccgccgg ccagcccggt gacgagggcc
      841 tgcagcgtgg cccagggcgt gggcagcagg atcgccttga tcaacccggc gcgcacgacc
      901 aggtcccaga cgatgaacag cgcgaccggg ccgacgaagg gcagcagccg gccccagctg
      961 aacttcgcgg ggcttgcggc ggacgcatcg gtcgagccgc tccacggctt cgcggccgcg
     1021 acgatggtgg tggtgtcgcc catcgctcag gccttgtaga gcagcggttc gacggcgacc
     1081 ttcttctcga agatgccctt gtccgagaac aggtcgtaga acttctggaa gtacgccacg
     1141 tcgctggcct tgaactcgct gtagagcatg tacgacgcca gcggcacctc ggcggtcagc
     1201 gcgccctcga tcgcggtgta gcccttcatg aacaggcgtg cctcctcggg tttgctgcgc
     1261 accagctcga tgccgcgtgc ataggcggcg atgaacttct tcgcttcggc cgggttcttc
     1321 ttgatgaact cgctcgtcag gctcgccgag ccgccatgcc agggcgccat cgggtcgccg
     1381 aggatgtagt gcgcgacgac accggcctcg agcacgcgcg tcgtgccgtt catgcggccg
     1441 atcgtgccgg tcggctcgag cgtgtaggcg gcgtcgatct ggccggccgc gagcgccgcg
     1501 acgtgctggc cgatcggcaa ctcggtgacg ctcatcgcgc cggcgccggc gcgctcgagc
     1561 atggtcttgg ccagcgtcac gttctggatg ccggggccgg aggcgacgcg tttgcccttc
     1621 agttcggcca tcgtcttgat cgggctgtcc ttgggcagca gaaactcctc gagcacgtgc
     1681 ttcgcattgc tggggttcgt cgcgaagatc ttgaacaggc ccggctgcgc gatctcgccg
     1741 atcgccaggt tcgccgagcc ggtgccgttg gcgctgccgt cggagcggcc cgagagcatc
     1801 gcctccatca cctgctgcgc gccggcgaac ttgagcggct cgacctcgag gccggcctcc
     1861 ttgaagtagc ccttctcgat cgccgcgaag aacggcagcc cggccgccac cggccagtag
     1921 ccgacgcgga tcttcggccc ggcctgcgcg cgcaccagcg cgggggcgcc gacgacggcg
     1981 agcgcactgc cgcaggcggc ggcgccgagc agccggcgac gggtgg
//
LOCUS       DATMJB010000696         7138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_228336, whole genome shotgun sequence.
ACCESSION   DATMJB010000696 DATMJB010000000
VERSION     DATMJB010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 7138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7138
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228336"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VNU48_14700"
     CDS             complement(<1..266)
                     /locus_tag="VNU48_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HWI12581.1"
                     /translation="MALDIVIMAAGKGTRMRSARPKVLHQLAGRSLLQHVLRTAAELG
                     ADGIVAIVGHGADEVEASVAAPDLPTLRFARQMPQLGTGHAVQQA"
     gene            265..1182
                     /locus_tag="VNU48_14705"
     CDS             265..1182
                     /locus_tag="VNU48_14705"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HWI12582.1"
                     /translation="MLSVFRSFGLRALIIVALCGVLGLAAGCSALRIAYGTAPDLAYW
                     WLDRYVDFSATQTPRVREAIRQWFAWNRRTQVPDYALLLARARVEVLADTTPARVCAW
                     QAEAMVRARVGFDRIAPAAADLMLTITPQQTRYLERRYDKFNAEFRDDFLQPDPRKRA
                     DEAFRRTLDRAETLYGRLDDAQRQRIVDGLKRSPFDPELWFAERRQRQQDALQILRRF
                     ATEGASREQALAALRGYVDRLERSPRDSYRRYSERLTEYNCAFAAGLHNATTAAQRRL
                     AANKLAGWEDDLRAIAMADPMPDTGRREP"
     gene            complement(1184..1810)
                     /locus_tag="VNU48_14710"
     CDS             complement(1184..1810)
                     /locus_tag="VNU48_14710"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HWI12583.1"
                     /translation="MPRLLAAAALALALGACASFNNLNNEVSTYGSWPADRRPASFVF
                     ERLPSQQAHPERRQQLEDAARGALEAAGFRAAADANDAQYLMQVGARVVSNDPWIYND
                     PLFWRGGFRHGRFGRAGFWGPGWGPGWGPGWGWGGPYDTPTYEREVALLIRDRKTGTL
                     LYEARANNSGPSAAIDYLLPAMFDAALKNFPNAGPNPQQVTTQISKRS"
     gene            complement(1840..2748)
                     /gene="ttcA"
                     /locus_tag="VNU48_14715"
     CDS             complement(1840..2748)
                     /gene="ttcA"
                     /locus_tag="VNU48_14715"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HWI12584.1"
                     /translation="MSAVLDSSTKDAKPSVEIHKLGKRLHRLVGQAIVDFNMIEEGDK
                     VMVCVSGGKDSYALLDILLALRARAPIRFDIVAVNLDQKQPGFPAHVLPEYLAKLGVP
                     FHIEDQDTYSIVKRVIPEGKTMCSLCSRLRRGILYRVAGELGATKIALGHHRDDMLQT
                     LFMNMFFGAKLKGMPAKLVSDDGRNIVIRPLAYVNEVDLERWAAHRQFPIIPCTLCGS
                     QDNLQRVQTKQMLRDWDRRFPGRIDNMFNAMANVVPSHLMDRNLYPFTTLKASGVADA
                     AGDRAFDDDESCAPPAAATITLRSSG"
     gene            complement(2745..3146)
                     /locus_tag="VNU48_14720"
     CDS             complement(2745..3146)
                     /locus_tag="VNU48_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HWI12585.1"
                     /translation="MHSLLSHPSLMDCRRLFLRDYEVFINIGVHAFEKTGEQRVLINV
                     DLYIPLALSTPKADELDEVVDYDFMRRSIASRIVRGHIHLQETLCDDVLALMLAHPKV
                     RAARVSTEKPDVYPDCDAVGCEVFGLKEKIA"
     gene            complement(3227..4018)
                     /locus_tag="VNU48_14725"
     CDS             complement(3227..4018)
                     /locus_tag="VNU48_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWI12586.1"
                     /translation="MSASSRPVVLVTGAARRLGRAMALELAAHSFDVAVHYRGSADDA
                     RATVAELRALGAGAEAFQADLSDEAACRALVPAVAAHFQRLDAVVNSASVFEYDDPAS
                     FSIAAMDKHWRANTAPAVILAQALHVLLAGCSEDQAKSVGCVVNIIDQKLWNPNPDYF
                     SYTLSKAALQAATVMLAQALAPSVRVCGVAPGVTLPSGPMDAQEFAAAHQMTPLRRSS
                     TPEDIARTVRFLIESPAITGTTLLVDGGQHLTAQARDVLYLAKKS"
     gene            4037..5146
                     /locus_tag="VNU48_14730"
     CDS             4037..5146
                     /locus_tag="VNU48_14730"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWI12587.1"
                     /translation="MQASESASVSADLPRLIHAALARDGGWLPFDRFMALALYAPGLG
                     YYANHGRKFGLLPQSGSDFVTAPELSPLFGQALARQVAQALRGSDTGELWEFGAGSGA
                     LAAQLLEALGAQVERYTIVELSGGLRERQRERLALHGDRVRWVDALPDAMAGVIVGNE
                     VLDAMPVQLLHFDGTRWLERGVVADGAAFAWSDRPTALAPPFEGSFAPGTVTEIHRQA
                     EAFVATLAERLTRGAAFFIDYGFPRSEYYHPQRSGGTLMCHRAHRADTDPLADVGAKD
                     ITAHVDFSAIALAAQDAGFEVLGYTTQAHFLINCGVMELLADADLKARANAQKLLTEH
                     EMGELFKVIGFAKGCDAAFMATPIGFARGDRTHTL"
     gene            5143..5337
                     /locus_tag="VNU48_14735"
     CDS             5143..5337
                     /locus_tag="VNU48_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HWI12588.1"
                     /translation="MIRWLLVVFLALILFSGLRPWLEKFGLGKLPGDFRFRLFGREWF
                     VPFASSVVLSLLAMLLIRIL"
     gene            5446..>7138
                     /locus_tag="VNU48_14740"
     CDS             5446..>7138
                     /locus_tag="VNU48_14740"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWI12589.1"
                     /translation="MGQGIATSYVQLAVDVFGVPPERIRVLQGDTDRANGFGSAGSRS
                     LFTGGSAVRVASERTIAKSKERAGEVLEVAPADIEYLEGRFKVVGTDVGIGLFELAGK
                     QPEQHIYLDSTSAVGGPTWPNACHVCEVEIDPGTGHVAVVGYASVNDIGRVVSPAIVT
                     GQIEGGAVQGIGQALSEQIVYDRDSGQILTASFQDYAMPHADVASSFFKTRFDTSIPC
                     TTNLLGVKGVGELGTIGATPATVKRGDRRARSRRPRPRHRACADAVDAAARVACAGRR
                     VRPVAVRLKRRGEPADPLNAGAPSRLREGRSCVISIASRGSTSIDACGSRPLVPRRAG
                     TTHNLRRSPMKVSGSRARVTVIAAAASLMAASAMAGIGVTPTTDPTTLGTALGGSGVT
                     INSVTVTNGAASQFGTYTGFNGSVVTLGNGIVLSSGKAVDTANVGTFIDTTTGAAGTS
                     EFDAYGAGHVANFSDSNDVAALKVSFTLASASAVSFSFAFGSMEFPDYVNDFTDAFLV
                     FLDGTGVANQIVFDASSNPVQVGSSFASSLVTTDASTAFTDPHGLVGVLTTTTGTL"
BASE COUNT         1061 a         2467 c         2488 g         1122 t
ORIGIN      
        1 gcctgctgca ccgcgtggcc ggtgccgagc tgcggcatct ggcgcgcaaa acgcaaggtc
       61 ggcaggtccg gcgcggcgac cgaagcctcg acctcgtcgg cgccgtggcc gacgatcgcc
      121 acgatgccat ctgcccccag ctcggcagcg gtgcgcagaa catgctgcaa taggctgcgt
      181 ccggccagct ggtgcagcac cttcggccgg gccgagcgca tccgcgtgcc cttgcccgcg
      241 gccatgatca cgatgtcgag tgccatgttg tctgtctttc gaagcttcgg gttgcgcgcg
      301 ctgattatcg tggcgctgtg cggtgtgctc ggcctcgccg cgggctgcag cgcgctgcgc
      361 atcgcctacg gcaccgcgcc cgacctggcc tactggtggc tggaccgcta tgtcgatttc
      421 agcgccacgc agacgccgcg cgtgcgcgag gccatcaggc agtggttcgc ctggaaccgc
      481 cgcacccagg tgcccgacta cgcgctgctg ctcgcacgcg cccgggtcga ggtgctggcc
      541 gacacgacgc cggcgcgtgt ctgcgcctgg caggccgagg cgatggtgcg tgcccgtgtc
      601 gggttcgacc ggatcgcacc ggccgctgcc gacctgatgc tgacgatcac gccgcagcag
      661 acccggtacc tcgagcgccg ctacgacaaa ttcaacgccg agttccgcga cgacttcctg
      721 cagcccgacc cgcgcaagcg cgcagacgag gccttcaggc gcacgctcga ccgcgccgag
      781 acgctctacg gccggctcga cgacgcgcag cgccagcgca tcgtcgacgg gctgaagcgc
      841 tcgccgttcg accccgagct gtggttcgcc gaacgccgcc agcgccagca ggacgccttg
      901 cagatcctgc gccggttcgc gaccgagggc gcgagccgcg agcaggcgct cgccgcactg
      961 cgaggctacg tcgaccgcct cgaacgctcg ccgcgcgaca gctaccggcg ctacagcgag
     1021 cgcctgaccg agtacaactg tgcgttcgcc gccggcctgc acaacgcgac gaccgcggca
     1081 cagcgccgct tggccgcgaa caagctggcg ggctgggagg acgacctgcg cgcgatcgcc
     1141 atggcggatc cgatgcccga cacgggacgc cgcgagcctt gagctaactg cgcttggaga
     1201 tctgcgtcgt cacctgctgc gggttcgggc cggcgttcgg gaagttcttc agcgcggcat
     1261 cgaacatcgc cggcagcagg tagtcgatcg ccgccgacgg cccgctgttg ttggcgcgcg
     1321 cctcgtacag cagcgtgccg gtcttgcggt cgcggatcag cagcgcgacc tcgcgttcgt
     1381 aggtcggcgt gtcgtacggg ccgccccagc cccagcccgg accccacccg gggccccagc
     1441 ccgggcccca gaaccccgca cggccgaagc ggccgtggcg gaagccgccg cgccagaaca
     1501 gcgggtcgtt gtagatccac gggtcgttgc tgacgacgcg cgcgccgacc tgcatcaggt
     1561 actgcgcgtc gttggcatcc gccgcggcgc gaaagcccgc cgcctcgagc gcgccgcgcg
     1621 ccgcgtcctc gagctgctgg cgccgttccg gatgcgcctg ctgcgagggc agccgctcga
     1681 acacgaagct cgccggccgg cggtcggccg gccagctgcc ataggtcgag acctcgttgt
     1741 tcaggttgtt gaaggaggcg caggcgccga gcgcgagagc gagggcggca gcggcaagca
     1801 gtctgggcat gatgttctct ccttcggccg ccgggcacgt caccccgagg aacgcagggt
     1861 gatcgttgcc gcggccggcg gcgcgcaaga ctcgtcgtcg tcgaaggcgc ggtcgccggc
     1921 ggcatcggcc acgccgctgg ctttgagcgt ggtgaacggg tagaggttcc ggtccatcag
     1981 gtgcgagggc acgacgttgg ccatcgcgtt gaacatgttg tcgatgcgcc ccggaaagcg
     2041 ccggtcccag tcgcgcagca tctgcttggt ctgcacgcgc tgcaggttgt cctggctgcc
     2101 gcacagcgtg cacgggatga tcgggaactg gcggtgggcg gcccagcgtt cgaggtcgac
     2161 ctcgttcaca taggcgagcg ggcggatcac gatgttgcgg ccgtcgtcgc tgaccagctt
     2221 ggccggcatg cccttcagct tggcgccgaa gaacatgttc atgaacaagg tttgcaacat
     2281 gtcgtcacgg tggtgaccga gcgcaatctt ggtcgcgccc agttcgccgg cgacccggta
     2341 caggatgccg cgccgcagcc gcgagcacag gctgcacatc gtcttgcctt ccgggatcac
     2401 gcgcttgacg atcgaatacg tgtcctggtc ctcgatgtga aacggcacgc cgagcttcgc
     2461 gaggtactcg ggcagcacgt gggccgggaa gccgggctgc ttctggtcga ggttgaccgc
     2521 gacgatgtcg aagcggatcg gcgcgcgcgc acgcagggcc agcaggatgt cgagcagcgc
     2581 gtagctgtct ttgccgcccg agacgcagac catgaccttg tcgccttctt cgatcatgtt
     2641 gaagtcgacg atggcctggc cgaccaggcg gtgcagccgc ttgccgagct tgtgaatctc
     2701 gaccgacggc ttggcgtcct tcgtggatga gtcgagcacg gcgctcatgc aattttctcc
     2761 ttcaggccga acacttcgca gccgaccgcg tcgcagtccg ggtacacgtc gggcttttcg
     2821 gtcgagacgc gcgcggcgcg caccttcggg tgcgccagca tcagcgccag cacgtcgtcg
     2881 cacagcgtct cctgcagatg gatgtggccg cggacgatgc gcgaggcaat gctgcggcgc
     2941 atgaagtcgt agtcgacgac ctcgtcgagc tcgtcggcct tcggcgtcga cagcgccagc
     3001 ggaatgtaga ggtcgacgtt gatcagcacg cgctgctcgc cggtcttctc gaacgcgtgc
     3061 acgccgatgt tgatgaagac ctcgtagtcg cgcaggaaca ggcgccggca gtccatcagg
     3121 ctggggtggc tcagcaggct gtgcatgtca gtcccgccgg gccgccccaa ggggctgacg
     3181 cgccccctcg gggggcagtg aacgaagtga gcgtgggggt ttcatcctag ctcttcttcg
     3241 caaggtagag cacgtcgcgc gcttgtgcgg tgaggtgctg accgccgtcg accagcagcg
     3301 tcgtgccggt gatcgccggc gattcgatca ggaagcgcac cgtgcgagca atgtcctcgg
     3361 gcgtcgacga gcgccgcagc ggcgtcatct ggtgcgccgc cgcgaactcc tgcgcgtcca
     3421 tcggccccga cggcagcgtg acgccgggtg cgacgccgca cacgcgcacg ctcggcgcga
     3481 gcgcctgcgc gagcatcacg gtggcggcct gcagcgcggc cttcgacagc gtgtacgaga
     3541 agtagtcggg gttcgggttc cagagcttct ggtcgatgat gttgaccacg cagccgacgc
     3601 tcttcgcctg atcttcgctg cagccggcca gcagcacgtg cagtgcctgc gccaggatca
     3661 ccgccggcgc ggtgttggcg cgccagtgct tgtccatcgc cgcgatgctg aagctggccg
     3721 ggtcgtcgta ctcgaagacc gaggcgctgt tgacgaccgc atcgaggcgc tggaaatgcg
     3781 ccgcgaccgc cggcaccagt gcgcggcagg ccgcctcgtc cgacaggtcg gcctggaagg
     3841 cttcggcgcc tgcgccgagc gcgcgcagtt cggccacggt cgcgcgcgca tcgtcggccg
     3901 agccgcggta gtgcaccgcc acgtcgaagc tgtgcgcggc cagctcgagc gccatcgcgc
     3961 ggccgagccg ccgggcggcg ccggtcacga gcacaacagg gcgggaagaa gcggacatgg
     4021 ttcctacaat gcgtcgatgc aagcgtctga atccgccagt gtatcggccg acctgcccag
     4081 gctgatccac gcggcgctgg cgcgcgacgg cggctggctg ccgttcgacc gcttcatggc
     4141 gctggcgctg tatgcgccgg gcctgggcta ctacgcgaac cacggccgca aattcggcct
     4201 gctgccgcaa tcgggcagcg atttcgtcac cgcgccggag ctttcgccgc tgttcggaca
     4261 ggcgctggcg cgtcaggtcg cgcaggcgct gcgcggcagc gacacgggcg aactgtggga
     4321 gttcggcgcc ggctccggcg cgctcgcggc ccagctgctg gaggcgctgg gcgcacaggt
     4381 cgagcgctac acgatcgtcg aactgtccgg cggcctgcgc gaacgccagc gcgagcgcct
     4441 cgcgcttcac ggcgaccggg tgcgctgggt cgacgcgctg cccgacgcga tggccggcgt
     4501 gatcgtcggc aacgaggtgc tcgacgcgat gccggtgcag ctgctgcatt tcgacggcac
     4561 gcgctggctc gaacgtggcg tcgtcgccga cggcgcggcg ttcgcctgga gcgatcggcc
     4621 gaccgcgctg gcgccaccat ttgaagggtc gttcgcgccc ggcaccgtga ccgagatcca
     4681 ccgccaggcc gaggccttcg tcgcgacgct ggccgagcgc ctgacccgcg gcgccgcgtt
     4741 cttcatcgac tacggcttcc cgcgctccga gtactaccac ccgcagcgca gcggcggcac
     4801 gctgatgtgc catcgcgcgc accgcgccga caccgacccg ctcgccgacg tcggcgcgaa
     4861 ggacatcacc gcgcacgtcg acttcagcgc catcgcgctg gccgcgcagg acgccgggtt
     4921 cgaggtgctc ggctacacga cgcaggcgca cttcctgatc aactgcggcg tgatggagct
     4981 gctggcggac gctgacctga aggcgcgcgc gaacgcgcag aagctgctga ccgagcatga
     5041 gatgggcgag ctgttcaagg tgatcggctt cgcgaagggc tgcgatgccg cgttcatggc
     5101 cacgccgatc ggcttcgccc gcggcgaccg gacgcacacg ctgtgatccg ttggctcctt
     5161 gtcgtcttcc tcgcgctgat cctgttcagc gggctgcgac catggctcga gaagttcggc
     5221 ctcggcaagc tgcccggtga ctttcgcttc cgcctgttcg ggcgcgaatg gttcgtgccg
     5281 ttcgcgagca gcgtcgtgct gagtctgctg gcgatgctgc tgatccggat tctctagggc
     5341 ctgttaacgc tatgggaggg ctcgcgccgg cgcctggcag gccccagggc tagctaggcg
     5401 cgagcgaggc ggcgtgcttt ggcacgcaac gagtgagcaa cggcgatggg ccagggcatc
     5461 gcgaccagct acgtgcagct cgcggtcgac gtgttcggcg tgccgcccga gcgcatccgc
     5521 gtgctgcagg gcgacaccga ccgggccaac ggcttcggca gcgccggttc gcgatcgctg
     5581 ttcaccggcg gctcggcagt gcgcgtcgcc tccgagcgca cgatcgcgaa gagcaaggaa
     5641 cgcgccggcg aggtgctcga ggtcgcgccg gccgacatcg aatacctcga aggccgcttc
     5701 aaggtcgtcg gcaccgatgt cggcatcggc ctgttcgagc tcgcgggcaa gcagcccgag
     5761 cagcacattt acctcgactc gaccagcgcg gtcggcggcc cgacgtggcc gaacgcctgc
     5821 catgtctgcg aggtcgagat cgacccgggc accgggcatg tcgccgtggt cggctacgcg
     5881 tcggtcaacg acatcggccg cgtcgtcagc ccggcgatcg tcaccggcca gatcgagggc
     5941 ggcgcggtgc agggcatcgg ccaggcactg agcgaacaga tcgtctacga ccgcgacagc
     6001 ggccagatcc tgaccgcgag cttccaggac tacgcgatgc cgcacgccga cgtggcgagc
     6061 agcttcttca agacgcgctt cgacacctcg atcccgtgca cgaccaacct gctcggcgtc
     6121 aagggtgtcg gcgaactcgg cacgatcggg gcgacgccgg cgaccgtcaa acgcggtgat
     6181 cgacgcgctc gatcacgccg gccacggccg cgccaccgag cgtgtgcaga tgccgttgac
     6241 gccgccgcgc gtgtggcgtg cgctggccgg cgagttcgac ccgtcgccgt tcggttgaag
     6301 cggcgcggtg agcccgcaga ccccctgaat gcgggggccc cctcccgtct tagggagggg
     6361 cgctcatgcg tgatctcgat agcgtcccgg gggtcgacat cgatcgatgc ctgcggctcg
     6421 cgcccactcg ttccaaggcg cgcgggcacg acacacaacc tcaggaggtc tcccatgaaa
     6481 gtttcaggtt cccgggcgcg cgttacggtg atcgccgcgg cggcatcgct gatggccgcg
     6541 tcggcgatgg ccggcatcgg cgtgacgccg acgaccgatc caaccaccct cggcactgcc
     6601 ttgggcggct ccggcgtgac gatcaactcc gtcaccgtga ccaacggtgc ggcgtcgcag
     6661 ttcggcacct acaccgggtt caacgggtcg gtggtcaccc tcggcaacgg catcgtgctg
     6721 tccagcggca aggccgtcga caccgcgaac gtgggcacat tcatcgacac caccaccggc
     6781 gcggcgggca cctccgagtt cgacgcctac ggcgccggcc acgtggcgaa cttcagcgac
     6841 tcgaacgacg tggccgcgtt gaaggtcagc ttcacgctgg cgtcggccag cgcggtgtcg
     6901 ttctcgttcg cgttcgggtc gatggaattt cccgattacg tcaacgactt caccgatgcg
     6961 ttcctggtct tcctcgacgg caccggcgtc gccaaccaga tcgtgttcga cgccagcagc
     7021 aacccggtcc aggtcggcag cagcttcgcc tcttcgctcg tgaccaccga cgcgagcacg
     7081 gccttcaccg acccgcacgg tctggtcggg gtcttgacca cgacaaccgg cacgctgt
//
LOCUS       DATMJB010000697         6038 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Burkholderiaceae bacterium isolate SMAG_U13557
            k141_214270, whole genome shotgun sequence.
ACCESSION   DATMJB010000697 DATMJB010000000
VERSION     DATMJB010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37942959
            Sequence Read Archive: SRR8205312
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiaceae bacterium (soil metagenome)
  ORGANISM  Burkholderiaceae bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Burkholderiaceae.
REFERENCE   1  (bases 1 to 6038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 17:41:04
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,955
            CDSs (total)                      :: 2,938
            Genes (coding)                    :: 2,907
            CDSs (with protein)               :: 2,907
            Genes (RNA)                       :: 17
            tRNAs                             :: 13
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 31
            CDSs (without protein)            :: 31
            Pseudo Genes (ambiguous residues) :: 0 of 31
            Pseudo Genes (frameshifted)       :: 6 of 31
            Pseudo Genes (incomplete)         :: 29 of 31
            Pseudo Genes (internal stop)      :: 0 of 31
            Pseudo Genes (multiple problems)  :: 4 of 31
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6038
                     /organism="Burkholderiaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214270"
                     /isolate="SMAG_U13557"
                     /isolation_source="soil"
                     /db_xref="taxon:2030806"
                     /environmental_sample
                     /country="China"
                     /lat_lon="43.2167 N 116.2333 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VNU48_14745"
     CDS             complement(<1..361)
                     /locus_tag="VNU48_14745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HWI12590.1"
                     /translation="MRVVLAASVFAAATAPSSAFAVDYLSVEQAARLMFPDADTFEAR
                     QVTLDAAQLQQLDAQGVRGRSARWPVSVARRGGTTLGFIVSDEVVGKVELISYAVGIA
                     LDGSIKQVEILSYRESHG"
     gene            complement(361..1707)
                     /locus_tag="VNU48_14750"
     CDS             complement(361..1707)
                     /locus_tag="VNU48_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWI12591.1"
                     /translation="MSMNPIRPRRATAQALVAATLALAAVSAFAQSGTQADLARRLDQ
                     LAAELADVKAQLARLQQRQPQAPAPASADAPPTATASAAATPAVAAPAPAYSASLLGP
                     GTVLTSYGELNYNRPTRANQNTQADVRRFVLGYQHRFDEKTKVVTELEVEHAVSSADD
                     PGEVEVEQAYVERQLTPVWSLRAGLFLMPVGLLNENHEPTAFYGVERNFVETAIIPST
                     WREGGAQLVGNFDSGLSVQAGVTTGFDLNKWDASSTEGQESPLGSIHQEMALAKAHDL
                     AVFGAANWRGMPGLLVGGSLFSGQATQAQAVDKARITLWDIHARWSPGRWDFSGVYTR
                     GTISNTAALNAALVGNPTLIPKSFDGVYVQGAYKLWSQGDYALSPFVRWEQFNTAKSY
                     ADLGPGLTPPAARAERVVTVGANLQVSQGIVLKADYQRFRENADLNRVDLGLGWSF"
     gene            complement(2003..2743)
                     /gene="fabG"
                     /locus_tag="VNU48_14755"
     CDS             complement(2003..2743)
                     /gene="fabG"
                     /locus_tag="VNU48_14755"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HWI12592.1"
                     /translation="MNPTAPTPRRALVTGASGGLGRAIAQRLARDGLHVIVHANTRLA
                     AAQALAAEIVAAGGSAEAIAFDLTDRAATHGACKQLLTGGAIQVLVNNAGTHDDAAFP
                     ALRAAQWDGVIDTSVNGFFNVTQPLTLPMIRTRWGRIVNISSVAALAGNRGQVNYAAA
                     KGALNSATKALALELASRGITVNAVAPGIIASGMADGAFDSAAIEQLVPMKRAGRADE
                     VAALVAFLASDDAGYISGQVISINGAMR"
     gene            complement(2740..3213)
                     /locus_tag="VNU48_14760"
     CDS             complement(2740..3213)
                     /locus_tag="VNU48_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HWI12593.1"
                     /translation="MHEPRSLTREGIAALIPHSGAMCLLARLDAWDPQHIVCSARNYR
                     DADHPLRTRRGLLAPCAIEYAAQAMALHGALIGQATGSPASPGYLASARGVRLHVLRL
                     DDLAPLDGAADELRVEATRQAGDARQILYAFTVRHGEQLLVEGRAAVVLNTVLAA"
     gene            complement(3192..4010)
                     /locus_tag="VNU48_14765"
     CDS             complement(3192..4010)
                     /locus_tag="VNU48_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HWI12594.1"
                     /translation="MSGLRVWVEGIGLLGPGLGSWSAAREMLRGTQPLATAATVLPAP
                     TRLPPAERRRAGAAVKLALAVADEAVAHAQADPAQLATVFASSSGEGTNCHALCETLA
                     SGTPMLSPTRFTNSVHNAASGAWHIAVGSRVASTSLCAFDGSFGAGLIEAATSIALSG
                     ATMLLVASDSPYPEPLNAVRPLPDHFGLALLLAPAATARSIASLTITLVGVDHAATRC
                     DDTAFETLRRAIPAAAALPLLIALAHAAPRRSVVLDYQAATRLQVDCECTSPAA"
     gene            complement(4072..5256)
                     /locus_tag="VNU48_14770"
     CDS             complement(4072..5256)
                     /locus_tag="VNU48_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HWI12595.1"
                     /translation="MKPLAISAATLTTALGAGNAATLDALRAQRSGLAPVRFAGLPLA
                     TWTGEVEGVETVVVPPSLTAYDCRNNRLAWLGLQQDGFLDAVAAARARWGARRVGVFI
                     GTSTSGLLQTELAYRRRSSDGALPADFDYRATQNTFSVADFAARALHLDGPAWVVSTA
                     CSSSAKVFGSAARLIAAGLIDAAVVGGVDSLCLTTLYGFNSLELLAPEGCRPWDAARD
                     GLSVGEAAAFALLERETGTPPQGWLLGVGESNDGYHMSTPHPQGLGAIAAMHAALADA
                     GLAPADIDYINLHGTATPSNDAAEDAAVASVFCTATPCSSTKGATGHTLGAAGGVEAA
                     IALLAMRHGLMPAGLNVAQRDPTLRMHYLLANRAATVRRVLSNSFGFGGSNASLVLGA
                     AE"
     gene            complement(5253..6035)
                     /locus_tag="VNU48_14775"
     CDS             complement(5253..6035)
                     /locus_tag="VNU48_14775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWI12596.1"
                     /translation="MPPLLRASIACHVGAGALALAQPALWPWALGAVALDHALITGAG
                     LWPRSTWLGSNLRRLPPAAIARREIALTIDDGPDPAVTPAVLDLLDAQRVRATFFCVA
                     RQAQQHAALCREIVRRGHSVQNHSFTHAHTFSLLGPQGVRRELVQAQDALAQITGTAP
                     RFFRAPAGLRNVFLAPALHELGLQLASWTRRGLDTARRDPSVVLARLTRGLAAGDILL
                     LHDRHAAVAASGRAVSLDVLPALLQRFERAGLRAVTLTEALA"
BASE COUNT          827 a         2157 c         2242 g          812 t
ORIGIN      
        1 agccatggct ctcgcgatac gacaggatct cgacctgctt gatgctgccg tccagggcga
       61 tgcccaccgc gtagctgatc agctcgacct tgccgacgac ctcgtcgctg acgatgaacc
      121 cgagcgtcgt gccgccgcgc ctggccacgc tcacgggcca ccgggccgag cggccccgca
      181 cgccctgcgc atcgagctgc tgcaactgcg cggcatcgag cgtgacctgg cgcgcttcga
      241 acgtgtcggc gtcggggaac atcagcctgg cggcttgttc gacactgagg tagtcgaccg
      301 cgaaggcgct gctcggcgcc gtcgccgccg cgaacaccga ggccgccagc accacgcgca
      361 tcagaagctc cagcccaggc cgaggtcgac gcggttcagg tcggcgttct cgcggaagcg
      421 ctggtagtcg gccttcagaa cgatgccctg cgagacctgc aggttcgcgc cgaccgtcac
      481 gacgcgctcg gcccgcgccg ccggcggcgt cagcccgggg ccgagatcgg cgtaggactt
      541 ggcggtgttg aactgctccc agcgcacgaa cggcgacagc gcatagtcgc cctgcgacca
      601 gagcttgtag gcgccctgaa catagacgcc gtcgaacgat ttcgggatca gcgtcgggtt
      661 gccgacgagc gcggcattca gcgcggcggt gttcgagatc gtgccgcgcg tgtagacgcc
      721 ggagaagtcc cagcgccccg ggctccagcg cgcgtggatg tcccacagcg tgatgcgggc
      781 cttgtccacc gcctgcgcct gcgtggcctg gccgctgaag agcgagccgc cgaccagcag
      841 acccggcatg ccgcgccagt tcgcggcgcc gaagaccgcg aggtcgtgag ccttcgccag
      901 cgccatctcc tgatggatcg agccgagcgg cgactcctgg ccctcggtcg aactcgcatc
      961 ccatttgttc aggtcgaagc cggtcgtcac gcccgcctgc acgctcaggc cgctgtcgaa
     1021 attgccgacg agctgcgccc cgccttcgcg ccaggtgctg gggatgatcg cggtctcgac
     1081 gaagttgcgt tcgacgccgt agaacgcggt cggctcgtgg ttctcgttca gcaggccgac
     1141 cggcatcagg aacagcccgg cgcgcagcga ccagaccggc gtcagctggc gttcgacata
     1201 cgcctgctcg acctcgacct cgccgggatc gtcggccgaa gagaccgcat gctcgacttc
     1261 cagttcggtc acgaccttgg tcttctcgtc gaagcggtgc tggtagccca gcacgaagcg
     1321 gcgcacgtcg gcctgggtgt tctggttggc gcgggtcgga cggttgtagt tgagctcgcc
     1381 gtagctcgtc agcaccgtgc cggggccgag cagcgaagcg gaataggccg gcgccggcgc
     1441 ggccacggca ggcgtcgcag ctgcggaagc ggtcgcggtc gggggcgcgt cggcggaggc
     1501 gggcgcgggc gcctgcggct gccgctgctg gagccgcgcg agctgtgcct tgacgtcggc
     1561 gagttcggcg gcgagctggt cgagccggcg tgcgagatcg gcctgcgtgc cgctctgcgc
     1621 gaaggcgctg acggcggcaa gcgcgagcgt ggcggccacc agtgcctgcg cggtcgcgcg
     1681 tcggggtcgg atcgggttca tgctcatgcg aaattctccg ggtgggcggc gttcgttctt
     1741 gcggccgccc cgctcgcggc cgggccacga cggggacgga acgagaactg ctaatacgaa
     1801 tggttcgcaa tcatatctcg acaccgacgc cgccgacagg acgaacgcgg cgaatgcgtt
     1861 gcgtcgatgc tgcaacggcg ttagcgtcat cggtctggcg acgccactac gaacgcctgc
     1921 atctgcacac cgacaggcgg cactccggcc tgcccggcag accgcgcccc ggccgcgttg
     1981 acgcagccgt cgcgaagacg cgctaccgca tcgcgccgtt gatcgagatc acctgcccgc
     2041 tgatatagcc cgcatcgtcc gatgcgagaa acgccaccag cgccgcgacc tcgtcggcgc
     2101 ggccggcgcg cttcatcggc acgagctgct cgatcgccgc gctgtcgaag gcgccgtcgg
     2161 ccatgcccga cgcgatgatg ccgggtgcga ccgcattgac cgtgatgccg cggctcgcga
     2221 gttcgagcgc gagcgccttg gtcgcgctgt tcagcgcacc cttggcggcc gcgtagttga
     2281 cctggccgcg gttgccggcg agcgcggcca ccgacgagat gttgacgatg cggccccagc
     2341 gcgtgcggat catcggcagg gtcagcggct gcgtgacgtt gaagaagccg ttcaccgagg
     2401 tgtcgatgac gccgtcccac tgcgccgcgc gcagcgccgg gaacgccgcg tcgtcgtgcg
     2461 tgccggcgtt gttgaccagc acctggatcg cgccgcccgt gagcagctgc ttgcaggcgc
     2521 cgtgggtggc ggcgcggtcg gtcaggtcga acgcgatcgc ttcggcgctg ccgccggcgg
     2581 caacgatctc tgcggccagc gcctgggccg ccgcaaggcg cgtgttcgca tgcacgatca
     2641 catgaaggcc gtcgcgcgcc aggcgctgcg cgatcgcgcg gccgaggccg ccgctggcgc
     2701 cggtgacgag ggcgcgccgc ggcgtcggtg cggtcgggtt catgcagcga gcaccgtgtt
     2761 caacacgacc gccgcgcgcc cttcgacgag cagttgctcg ccgtggcgga ccgtgaacgc
     2821 gtacaggatc tggcgcgcat cgccggcctg gcgcgtcgcc tcgacgcgca gctcgtcggc
     2881 agcgccatcg agcggcgcca ggtcgtcgag ccgcagcacg tgcagccgca cgccgcgcgc
     2941 gctcgccagg tagccggggc tggccggcga gcccgttgcc tggccgatca gcgcgccatg
     3001 cagtgccatc gcctgcgcgg cgtattcgat cgcacagggc gcgagcaggc cgcggcgggt
     3061 gcgcagcggg tggtcggcgt cgcggtagtt gcgggcgctg cagacgatgt gctgcgggtc
     3121 ccacgcgtcc aggcgcgcca gcaggcacat cgcgccgctg tgcggaatca gcgcggcgat
     3181 gccttcgcgc gtcaggctgc ggggctcgtg cattcgcaat cgacctgcag ccgcgtcgcc
     3241 gcctggtagt cgagcacgac gctgcgccgc ggcgcagcgt gcgccagcgc gatcagcagc
     3301 ggcagcgcgg ccgcggcggg gatcgcgcgc cgcagcgtct cgaacgcggt gtcgtcgcag
     3361 cgcgtggccg cgtggtcgac accgacgagc gtgatcgtca gcgacgcgat cgaacgcgcg
     3421 gtggcggccg gcgcgagcag cagcgccaag ccgaagtgat cgggcagcgg ccgcaccgca
     3481 ttgagcggtt cggggtaggg cgaatcgctc gcgaccagca gcatggtcgc gccgctcagc
     3541 gcaatgctcg ttgcggcctc gatcagcccg gcgccgaaac tgccgtcgaa cgcgcacagg
     3601 ctggtcgacg ccacgcggct gcccacggcg atgtgccacg cgccggaggc cgcgttgtgc
     3661 accgagttcg tgaagcgcgt cggcgacagc atcggcgtgc cgctggccag cgtctcgcac
     3721 agcgcatggc agttcgtgcc ctcgccgctg ctcgaggcga acaccgtggc gagttgcgcg
     3781 gggtcggcct gcgcgtgcgc gaccgcctcg tcggccaccg ccagcgcgag cttgaccgcg
     3841 gctccggcgc ggcgccgctc ggccggcggc aggcgcgtcg gcgcgggcag cacggttgct
     3901 gcggtggcga gcggctgcgt gccgcgcagc atctcgcgcg cggcgctcca cgagcccagc
     3961 cccggcccga gcaggccgat gccctcgacc cagacgcgca agccgctcat cgcgcaacct
     4021 ccgtgccgcg cactgcggtg cgagccccct tcggagcggc cgggcggggg ctcattcggc
     4081 ggccccgagc accaggctcg cgttcgagcc gccgaagccg aacgagttcg acaacacgcg
     4141 gcgcacggtc gccgcgcggt tcgcgagcag gtagtgcatg cgcagcgtcg gatcgcgttg
     4201 cgccacgttc aggcccgccg gcatcaggcc gtggcgcatc gccagcagcg cgatcgccgc
     4261 ctcgacgccg ccggccgcgc cgagcgtgtg gccggtcgcg cccttggtcg agctgcaggg
     4321 cgtggccgtg caaaacacac tcgcgaccgc agcgtcctcc gctgcatcgt tgctcggcgt
     4381 cgcagtgccg tgcaggttga tgtagtcgat gtcggccggc gccaggccgg cgtcggcgag
     4441 tgccgcgtgc atcgccgcga tcgcgcccag gccctgcggg tgcggcgtgc tcatgtggta
     4501 gccgtcgttg ctttcgccga cgccgagcag ccagccctgc gggggcgtgc cggtctcgcg
     4561 ctcgagcagc gcgaacgcgg cggcctcgcc gaccgacagg ccatcgcgcg ccgcgtccca
     4621 gggccggcag ccctcggggg ccagcagctc cagcgagttg aagccgtaca gcgtggtcag
     4681 gcacaggctg tcgacgccgc cgaccacggc cgcgtcgatc aggccggccg cgatcaggcg
     4741 tgccgcgctg ccgaacacct tcgcgctgga cgagcaggcg gtcgagacga cccaggcggg
     4801 cccgtccagg tgcagcgcgc gcgccgcgaa atccgcgacc gagaaggtgt tctgcgtcgc
     4861 cctgtagtcg aagtcggccg gcagcgcgcc gtcgctgctg cgccggcgat aggcgagctc
     4921 ggtctgcagc agccccgagg tgctggtgcc gatgaacacg ccgacgcgcc gcgcgcccca
     4981 gcgcgcgcgc gccgcggcca ctgcgtcgag aaacccgtcc tgctgcaggc cgagccaggc
     5041 caggcggttg ttgcggcagt cgtaggcggt caggctgggc ggaacgacga ccgtctcgac
     5101 gccctcgacc tcgccggtcc aggtggccag cggcagaccg gcaaagcgca ccggcgccag
     5161 gccgctgcgc tgcgcccgca acgcgtccag ggtcgcggcg ttgccggcgc cgagcgcggt
     5221 cgtcagcgtg gcggcactga tcgcgagcgg cttcatgcga gggcctcggt caaggtcacc
     5281 gcgcgcagcc cggcccgctc gaagcgctgc agcagggccg gcaacacgtc cagcgaaaca
     5341 gcccggccgc tggccgcgac tgccgcgtgg cggtcgtgca gcagcaggat gtcgccggcc
     5401 gcgaggccgc gcgtcaggcg cgcgagcacg accgacgggt cgcggcgcgc ggtgtcgagg
     5461 ccgcgccgcg tccagctcgc cagttgcaga ccgagctcgt gcagcgccgg cgcgagaaag
     5521 acgttgcgca acccggccgg cgcgcggaag aagcgtggcg ccgtgccggt gatctgcgcg
     5581 agtgcgtcct gcgcctgcac gagctcgcgg cgcacgccct gcgggccgag cagcgagaag
     5641 gtgtgcgcgt gcgtgaagct gtggttctgc acgctgtggc cgcgccgcac gatctcgcgg
     5701 cacagcgcgg catgctgctg cgcctgacgc gcgacgcaga agaaggtggc gcgcacgcgc
     5761 tgcgcgtcga gcaggtccag caccgcgggc gtgacggccg gatcggggcc gtcgtcgatc
     5821 gtcagcgcga tctcgcgacg cgcgatggcc gccggcggca ggcgccgcag gttgctgccc
     5881 agccaggtcg agcgcggcca caggccggcg ccggtgatca gtgcgtggtc gagcgcgacg
     5941 gcaccgagcg cccacggcca cagcgccggc tgcgccagcg ccagcgcccc cgcgcccaca
     6001 tggcaggcga tgctggccct gagcagcggc ggcaggcg
//
